pik3c2a cDNA ORF clone, Danio rerio(zebrafish)

The following pik3c2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pik3c2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa146753 XM_021474429.1
Latest version!
Danio rerio phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha (pik3c2a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa146753 XM_021474430.1
Latest version!
Danio rerio phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha (pik3c2a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa146754 XM_021474431.1
Latest version!
Danio rerio phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha (pik3c2a), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa146022 XM_005159306.3
Latest version!
Danio rerio phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha (pik3c2a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa146022 XM_005159305.4
Latest version!
Danio rerio phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha (pik3c2a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa146023 XM_694937.8
Latest version!
Danio rerio phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha (pik3c2a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa23385 XM_021467820.1
Latest version!
Danio rerio phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha (pik3c2a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa23385 XM_005159305.3
Latest version!
Danio rerio phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha (pik3c2a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa23385 XM_021467821.1
Latest version!
Danio rerio phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha (pik3c2a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa23385 XM_005159306.2
Latest version!
Danio rerio phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha (pik3c2a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa23386 XM_694937.7
Latest version!
Danio rerio phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha (pik3c2a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa23386 XM_021467822.1
Latest version!
Danio rerio phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha (pik3c2a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa146753
    Clone ID Related Accession (Same CDS sequence) XM_021474429.1 , XM_021474430.1
    Accession Version XM_021474429.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3588bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCCACA TATCCAGTGG CAATGGGTTT AAGTTTGACA GGCCAGCCTC TCCGGGGGTG 
    GTCCGGCCAA AAGGAATTGT GGGGAAGGAA GAGGCTTTAC GCATGGAGGT AGAGGCCTTC
    GATAAAATTA AGCGCGAGAA AAGGCACACA CTCCCAGTCA CAGCCTCTTC TGTTAATCCC
    CTTCCCAATC CAACAGCGCA AACATCCAGC AGCCGGCCAG AGAAAGACCT TATTGTATTC
    TGCGAATCAG AAACACAGAA GAAGGAGCAA AAAGACAACT TTGAGGACAT CGATTTGGAG
    AACCTGACAA AGGAGGAGCT GGAAAAGCTG CTTCTGGATG ACAGTTTTGG TGCAAACAAA
    ATGACAAGAC CTTCCTCTTT GCTAGGTTGC AATCTCAGCG CATCATATCC TGGCGGACAT
    GCCTTCAACC CCTCTTCGTT CCACTGGACA CCCACTCCAA CGCATGCACA GACTCCCATC
    TTCCCCTCTG CTCCATTCGC CAAGCCTCCT TGCTCTTTCC AGAATGGCTT TAGTCCAGCA
    ATGTCTCCAT TTATTCCACC GACCCCCTTT CTCTCCTTTA CTCCCATCCA ACCACCTGCT
    GCTCTGGTCT ACTCGCAGCC TGCCGTCACC CCAGAGATGG CCAAGCTGTT TGACAAGATT
    GCCAGCACTT CGGAATACTT GAAGAACGGC AGGTCCTCTA GCATGGAAAC CGAATCGGCA
    AGTGTCAAGT CTCTGGAGCC CCTACCTCTG CCCTCAGAAC CTCCTAACAT CAGCCGATTT
    GAATGGCTGG ACTTGGACCC TCTCAACAAG CGCAAGGAGG TGGAGGTCGA GGAGACACCT
    GCTGTTTCAA GCTGTCCTTT TATTGAGGAG TCTGGGAAAG CTAAAGACCC GTGGGATGCG
    GTTCTTCAGG ATGAGCATGA AACTGTAGAT AATGGCAGTC CCACCGCAGA GGAAAAGAGC
    AAGGCAACTC AACCCAGAAG AGCATCCACA GGGGCGGCTG TAACAAAAAG CCAATCCCTC
    AGTATATCTG CAAACTCAAC GAATCAAAGC ACAGGCAAAC AGGTCAATAA GGGAAGCACA
    AATGTACTGT CCAAGTACTC TGCCTTACAG GAGAAGGAAG CCCAGAATCT AGAAGTTGTA
    GCATTCTGTG AAGACATTTC AAAACTGAGG TCAAAGTTCC CACACGATGA CCTGTCTACC
    AACCCTGGCT ATGTTCTCAG CCCTGTGATC ACGCAGAGGG ATGCAGGAGG GGACAATGGG
    GGCAGTGTGA AAGTGTCCAT TGAGATTTCT GATTCTCAAC AGCCTGTAAC GTTTACTTGC
    GATGTGACTT CTCCAGTGGA CTTGCTCATA ATGCAGGCTT TGTGTTGGGT TCATGATGAC
    CTGAACCAGG TGGACATCAA TAGCTACGTT CTCAAGGTCT GCGGACAGGA GGAGGTCCTT
    CAGAATAAAC ACAGTCTAGG CAGTCATGAG TACGTGCAGA ACTGCAGGAA GTGGGAATCA
    GAGATCAAAC TACAGCTCCT GTTTCTTAGC ACAATGAGAA GAGATCTGGC CCGAACTGCA
    GAAGATGACA TCTCGCCCAT TGATTTGGAG AAATACCTTG GTCTAGTGGA GAGGCCATTT
    AAAGAGGCAG TCACAAGAGA AGGCCTTGCT GAATATCTAG AAGGCTTTCA TAAGCAAGTC
    AATCTCTGCC TTCAGAATGA GAACAACCAA TATAAAACAG TGGACAATGT GGTTCAGTCT
    GTTAAGAATC TGTGCTGTGC TCTGGATGAA GTTGAGACAC CAGCCATCAC TGATGCCGTC
    AAGAGGCTCA AGCGCTCTGT CAACCTTCCT AGAACACGCT CACCAGAGCA CTATGCTGAA
    TTGCAGGCTG GTGCCACATC TTCCGGAGGT TCAGCTAATG GTTACTCTAG CCCTGTAGAG
    GAGAGTTTAG CAGTGCTCAC AGATGCTGTC TATGAACTCG CCAAGCTCTA CCTTCAGTCT
    TTCTGCCCGA TTAGCCCTGG TTTCATGATA GAAGAGCACA AAGAAGATGA GAGGGACAGC
    AAAGAGGCCT CGGGCACCAC TGAACACTTA CAGTTCACAC TGTTTGCCGT TCATGGCATT
    CCTGCTACCT GGGTCAGCAG TTTTGAAAAA TACTACCTGA TGTGTGCCCT GACCCATAAC
    AACAGGAATC TCTTCAAACC TGTCCAGTCC AAAAAAGTGG GAACATACAA GAGTTTCTTC
    TACCACATCA AATGGGATGA ATTAATAAAC TTCCCTATTT CAGTGTCGCT GTTACCACTG
    GAGGCTATGC TTAGTCTTTC TCTCTATGGG GTCCTCAGTC AGAATGCAAA CAACTCACCA
    GATTCCAACA AGCAACGGAA AGGTCCAGAA CTTTTGGGAA AAGTTTCCAT GCCTCTGTTT
    GACTTCAGGA GGGTTCTGTC CAGAGGCAGT AAACTGCTGA GTTTGTGGAC TTCTCCTCAA
    GCCCTTCAGC CTGGAGCTGC AGGCAAAGGG AGGAATCCTA CAGAAAAGAT CATACTGCAG
    GTTGACTTCC CCAGTCCAGC GGTGGACGTG CTCTATGTTG GTCCTCAGGA GAACGGCTGC
    CCCAATCCTC AGTCTCTAGA CCCACTTGAT CAAGACGATC GAAGTGAGAT AGAAAAGCTG
    TGCGCACGAG CATCTACTTT CGGACTGTCA CGGGCGGACA GACAGCTGCT TTGGGACCAG
    AGGTACTATT GTCGGGAATA TGAATACAGT TTGCCGAAAA TCCTGGCCAG CGCTCCCAGC
    TGGGACTGGG GCAGTATGGG GGAAATCCAC GCACTTCTGC ACCACTGGCC TGCTCTGTCA
    CCCGTTTCAG CTCTTGAGCT CCTTGAATCC AAGTTTGCCG ACACAGAGGT GAGGAAAGTG
    GCTGTGAGCT GGATCCAGAG CAGCAGTGAT GATGAGCTGG CTGATTATCT CCCCCAGCTA
    GTGCAGGCAG TGAAGTTCGA GTGTCACCTT AACAATGCCT TGGTGAAGTT TCTGCTGTCT
    CGAGCTCTGG GAAATGTCAA CATTGCACAT TATCTTTACT GGCTGCTGAG GGATGCAGTG
    CAGGACCCGG CGTTTGGTCA GCGTTATGAG CGGATCCTCT GTGTGCTGCT GTGTCTGTGT
    GGGACCGGAC TGAGGGCTGA GCTGGAGAAG CAGACCCGAC TTGTGCAGCT GCTTGGAGCT
    CTGGCTGAAA AGGTTCGGCA GGCGAGCAGC TCAACCCGAC AGGTGGTTCT CCTCGAGGGT
    CTAGAGAGGG TTCAGTCTTT CTTCCAGAAG AACAGCTGTC GACTCCCTAT TAGCCCCAGT
    CTGGTGGCAA AAGAGCTCAA TATCAAGGTC TGCTCCTTTT TCAATTCCAA CGCTGTACCT
    CTAAAGATCG CACTGGTTAA CGCAGATCCG CTGGGAGATG AGATTAACGT CATGTTTAAG
    GTTGGAGAAG ACCTGCGGCA GGACATGTTG GCACTACAGA TGATTCGGAT TATGGATCGG
    ATCTGGCTTC AGGAAGGACT CGACCTGCGT ATTGTTAACT TTAAATGCAT CTCTACTGGG
    AAAGATAAAG GTATGTTCCA CTCTACCAAA TTTACATTTG ACAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330104.1
    CDS177..3764
    Translation

    Target ORF information:

    RefSeq Version XM_021474429.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha (pik3c2a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474429.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCCACA TATCCAGTGG CAATGGGTTT AAGTTTGACA GGCCAGCCTC TCCGGGGGTG 
    GTCCGGCCAA AAGGAATTGT GGGGAAGGAA GAGGCTTTAC GCATGGAGGT AGAGGCCTTC
    GATAAAATTA AGCGCGAGAA AAGGCACACA CTCCCAGTCA CAGCCTCTTC TGTTAATCCC
    CTTCCCAATC CAACAGCGCA AACATCCAGC AGCCGGCCAG AGAAAGACCT TATTGTATTC
    TGCGAATCAG AAACACAGAA GAAGGAGCAA AAAGACAACT TTGAGGACAT CGATTTGGAG
    AACCTGACAA AGGAGGAGCT GGAAAAGCTG CTTCTGGATG ACAGTTTTGG TGCAAACAAA
    ATGACAAGAC CTTCCTCTTT GCTAGGTTGC AATCTCAGCG CATCATATCC TGGCGGACAT
    GCCTTCAACC CCTCTTCGTT CCACTGGACA CCCACTCCAA CGCATGCACA GACTCCCATC
    TTCCCCTCTG CTCCATTCGC CAAGCCTCCT TGCTCTTTCC AGAATGGCTT TAGTCCAGCA
    ATGTCTCCAT TTATTCCACC GACCCCCTTT CTCTCCTTTA CTCCCATCCA ACCACCTGCT
    GCTCTGGTCT ACTCGCAGCC TGCCGTCACC CCAGAGATGG CCAAGCTGTT TGACAAGATT
    GCCAGCACTT CGGAATACTT GAAGAACGGC AGGTCCTCTA GCATGGAAAC CGAATCGGCA
    AGTGTCAAGT CTCTGGAGCC CCTACCTCTG CCCTCAGAAC CTCCTAACAT CAGCCGATTT
    GAATGGCTGG ACTTGGACCC TCTCAACAAG CGCAAGGAGG TGGAGGTCGA GGAGACACCT
    GCTGTTTCAA GCTGTCCTTT TATTGAGGAG TCTGGGAAAG CTAAAGACCC GTGGGATGCG
    GTTCTTCAGG ATGAGCATGA AACTGTAGAT AATGGCAGTC CCACCGCAGA GGAAAAGAGC
    AAGGCAACTC AACCCAGAAG AGCATCCACA GGGGCGGCTG TAACAAAAAG CCAATCCCTC
    AGTATATCTG CAAACTCAAC GAATCAAAGC ACAGGCAAAC AGGTCAATAA GGGAAGCACA
    AATGTACTGT CCAAGTACTC TGCCTTACAG GAGAAGGAAG CCCAGAATCT AGAAGTTGTA
    GCATTCTGTG AAGACATTTC AAAACTGAGG TCAAAGTTCC CACACGATGA CCTGTCTACC
    AACCCTGGCT ATGTTCTCAG CCCTGTGATC ACGCAGAGGG ATGCAGGAGG GGACAATGGG
    GGCAGTGTGA AAGTGTCCAT TGAGATTTCT GATTCTCAAC AGCCTGTAAC GTTTACTTGC
    GATGTGACTT CTCCAGTGGA CTTGCTCATA ATGCAGGCTT TGTGTTGGGT TCATGATGAC
    CTGAACCAGG TGGACATCAA TAGCTACGTT CTCAAGGTCT GCGGACAGGA GGAGGTCCTT
    CAGAATAAAC ACAGTCTAGG CAGTCATGAG TACGTGCAGA ACTGCAGGAA GTGGGAATCA
    GAGATCAAAC TACAGCTCCT GTTTCTTAGC ACAATGAGAA GAGATCTGGC CCGAACTGCA
    GAAGATGACA TCTCGCCCAT TGATTTGGAG AAATACCTTG GTCTAGTGGA GAGGCCATTT
    AAAGAGGCAG TCACAAGAGA AGGCCTTGCT GAATATCTAG AAGGCTTTCA TAAGCAAGTC
    AATCTCTGCC TTCAGAATGA GAACAACCAA TATAAAACAG TGGACAATGT GGTTCAGTCT
    GTTAAGAATC TGTGCTGTGC TCTGGATGAA GTTGAGACAC CAGCCATCAC TGATGCCGTC
    AAGAGGCTCA AGCGCTCTGT CAACCTTCCT AGAACACGCT CACCAGAGCA CTATGCTGAA
    TTGCAGGCTG GTGCCACATC TTCCGGAGGT TCAGCTAATG GTTACTCTAG CCCTGTAGAG
    GAGAGTTTAG CAGTGCTCAC AGATGCTGTC TATGAACTCG CCAAGCTCTA CCTTCAGTCT
    TTCTGCCCGA TTAGCCCTGG TTTCATGATA GAAGAGCACA AAGAAGATGA GAGGGACAGC
    AAAGAGGCCT CGGGCACCAC TGAACACTTA CAGTTCACAC TGTTTGCCGT TCATGGCATT
    CCTGCTACCT GGGTCAGCAG TTTTGAAAAA TACTACCTGA TGTGTGCCCT GACCCATAAC
    AACAGGAATC TCTTCAAACC TGTCCAGTCC AAAAAAGTGG GAACATACAA GAGTTTCTTC
    TACCACATCA AATGGGATGA ATTAATAAAC TTCCCTATTT CAGTGTCGCT GTTACCACTG
    GAGGCTATGC TTAGTCTTTC TCTCTATGGG GTCCTCAGTC AGAATGCAAA CAACTCACCA
    GATTCCAACA AGCAACGGAA AGGTCCAGAA CTTTTGGGAA AAGTTTCCAT GCCTCTGTTT
    GACTTCAGGA GGGTTCTGTC CAGAGGCAGT AAACTGCTGA GTTTGTGGAC TTCTCCTCAA
    GCCCTTCAGC CTGGAGCTGC AGGCAAAGGG AGGAATCCTA CAGAAAAGAT CATACTGCAG
    GTTGACTTCC CCAGTCCAGC GGTGGACGTG CTCTATGTTG GTCCTCAGGA GAACGGCTGC
    CCCAATCCTC AGTCTCTAGA CCCACTTGAT CAAGACGATC GAAGTGAGAT AGAAAAGCTG
    TGCGCACGAG CATCTACTTT CGGACTGTCA CGGGCGGACA GACAGCTGCT TTGGGACCAG
    AGGTACTATT GTCGGGAATA TGAATACAGT TTGCCGAAAA TCCTGGCCAG CGCTCCCAGC
    TGGGACTGGG GCAGTATGGG GGAAATCCAC GCACTTCTGC ACCACTGGCC TGCTCTGTCA
    CCCGTTTCAG CTCTTGAGCT CCTTGAATCC AAGTTTGCCG ACACAGAGGT GAGGAAAGTG
    GCTGTGAGCT GGATCCAGAG CAGCAGTGAT GATGAGCTGG CTGATTATCT CCCCCAGCTA
    GTGCAGGCAG TGAAGTTCGA GTGTCACCTT AACAATGCCT TGGTGAAGTT TCTGCTGTCT
    CGAGCTCTGG GAAATGTCAA CATTGCACAT TATCTTTACT GGCTGCTGAG GGATGCAGTG
    CAGGACCCGG CGTTTGGTCA GCGTTATGAG CGGATCCTCT GTGTGCTGCT GTGTCTGTGT
    GGGACCGGAC TGAGGGCTGA GCTGGAGAAG CAGACCCGAC TTGTGCAGCT GCTTGGAGCT
    CTGGCTGAAA AGGTTCGGCA GGCGAGCAGC TCAACCCGAC AGGTGGTTCT CCTCGAGGGT
    CTAGAGAGGG TTCAGTCTTT CTTCCAGAAG AACAGCTGTC GACTCCCTAT TAGCCCCAGT
    CTGGTGGCAA AAGAGCTCAA TATCAAGGTC TGCTCCTTTT TCAATTCCAA CGCTGTACCT
    CTAAAGATCG CACTGGTTAA CGCAGATCCG CTGGGAGATG AGATTAACGT CATGTTTAAG
    GTTGGAGAAG ACCTGCGGCA GGACATGTTG GCACTACAGA TGATTCGGAT TATGGATCGG
    ATCTGGCTTC AGGAAGGACT CGACCTGCGT ATTGTTAACT TTAAATGCAT CTCTACTGGG
    AAAGATAAAG GTATGTTCCA CTCTACCAAA TTTACATTTG ACAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146753
    Clone ID Related Accession (Same CDS sequence) XM_021474429.1 , XM_021474430.1
    Accession Version XM_021474430.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3588bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCCACA TATCCAGTGG CAATGGGTTT AAGTTTGACA GGCCAGCCTC TCCGGGGGTG 
    GTCCGGCCAA AAGGAATTGT GGGGAAGGAA GAGGCTTTAC GCATGGAGGT AGAGGCCTTC
    GATAAAATTA AGCGCGAGAA AAGGCACACA CTCCCAGTCA CAGCCTCTTC TGTTAATCCC
    CTTCCCAATC CAACAGCGCA AACATCCAGC AGCCGGCCAG AGAAAGACCT TATTGTATTC
    TGCGAATCAG AAACACAGAA GAAGGAGCAA AAAGACAACT TTGAGGACAT CGATTTGGAG
    AACCTGACAA AGGAGGAGCT GGAAAAGCTG CTTCTGGATG ACAGTTTTGG TGCAAACAAA
    ATGACAAGAC CTTCCTCTTT GCTAGGTTGC AATCTCAGCG CATCATATCC TGGCGGACAT
    GCCTTCAACC CCTCTTCGTT CCACTGGACA CCCACTCCAA CGCATGCACA GACTCCCATC
    TTCCCCTCTG CTCCATTCGC CAAGCCTCCT TGCTCTTTCC AGAATGGCTT TAGTCCAGCA
    ATGTCTCCAT TTATTCCACC GACCCCCTTT CTCTCCTTTA CTCCCATCCA ACCACCTGCT
    GCTCTGGTCT ACTCGCAGCC TGCCGTCACC CCAGAGATGG CCAAGCTGTT TGACAAGATT
    GCCAGCACTT CGGAATACTT GAAGAACGGC AGGTCCTCTA GCATGGAAAC CGAATCGGCA
    AGTGTCAAGT CTCTGGAGCC CCTACCTCTG CCCTCAGAAC CTCCTAACAT CAGCCGATTT
    GAATGGCTGG ACTTGGACCC TCTCAACAAG CGCAAGGAGG TGGAGGTCGA GGAGACACCT
    GCTGTTTCAA GCTGTCCTTT TATTGAGGAG TCTGGGAAAG CTAAAGACCC GTGGGATGCG
    GTTCTTCAGG ATGAGCATGA AACTGTAGAT AATGGCAGTC CCACCGCAGA GGAAAAGAGC
    AAGGCAACTC AACCCAGAAG AGCATCCACA GGGGCGGCTG TAACAAAAAG CCAATCCCTC
    AGTATATCTG CAAACTCAAC GAATCAAAGC ACAGGCAAAC AGGTCAATAA GGGAAGCACA
    AATGTACTGT CCAAGTACTC TGCCTTACAG GAGAAGGAAG CCCAGAATCT AGAAGTTGTA
    GCATTCTGTG AAGACATTTC AAAACTGAGG TCAAAGTTCC CACACGATGA CCTGTCTACC
    AACCCTGGCT ATGTTCTCAG CCCTGTGATC ACGCAGAGGG ATGCAGGAGG GGACAATGGG
    GGCAGTGTGA AAGTGTCCAT TGAGATTTCT GATTCTCAAC AGCCTGTAAC GTTTACTTGC
    GATGTGACTT CTCCAGTGGA CTTGCTCATA ATGCAGGCTT TGTGTTGGGT TCATGATGAC
    CTGAACCAGG TGGACATCAA TAGCTACGTT CTCAAGGTCT GCGGACAGGA GGAGGTCCTT
    CAGAATAAAC ACAGTCTAGG CAGTCATGAG TACGTGCAGA ACTGCAGGAA GTGGGAATCA
    GAGATCAAAC TACAGCTCCT GTTTCTTAGC ACAATGAGAA GAGATCTGGC CCGAACTGCA
    GAAGATGACA TCTCGCCCAT TGATTTGGAG AAATACCTTG GTCTAGTGGA GAGGCCATTT
    AAAGAGGCAG TCACAAGAGA AGGCCTTGCT GAATATCTAG AAGGCTTTCA TAAGCAAGTC
    AATCTCTGCC TTCAGAATGA GAACAACCAA TATAAAACAG TGGACAATGT GGTTCAGTCT
    GTTAAGAATC TGTGCTGTGC TCTGGATGAA GTTGAGACAC CAGCCATCAC TGATGCCGTC
    AAGAGGCTCA AGCGCTCTGT CAACCTTCCT AGAACACGCT CACCAGAGCA CTATGCTGAA
    TTGCAGGCTG GTGCCACATC TTCCGGAGGT TCAGCTAATG GTTACTCTAG CCCTGTAGAG
    GAGAGTTTAG CAGTGCTCAC AGATGCTGTC TATGAACTCG CCAAGCTCTA CCTTCAGTCT
    TTCTGCCCGA TTAGCCCTGG TTTCATGATA GAAGAGCACA AAGAAGATGA GAGGGACAGC
    AAAGAGGCCT CGGGCACCAC TGAACACTTA CAGTTCACAC TGTTTGCCGT TCATGGCATT
    CCTGCTACCT GGGTCAGCAG TTTTGAAAAA TACTACCTGA TGTGTGCCCT GACCCATAAC
    AACAGGAATC TCTTCAAACC TGTCCAGTCC AAAAAAGTGG GAACATACAA GAGTTTCTTC
    TACCACATCA AATGGGATGA ATTAATAAAC TTCCCTATTT CAGTGTCGCT GTTACCACTG
    GAGGCTATGC TTAGTCTTTC TCTCTATGGG GTCCTCAGTC AGAATGCAAA CAACTCACCA
    GATTCCAACA AGCAACGGAA AGGTCCAGAA CTTTTGGGAA AAGTTTCCAT GCCTCTGTTT
    GACTTCAGGA GGGTTCTGTC CAGAGGCAGT AAACTGCTGA GTTTGTGGAC TTCTCCTCAA
    GCCCTTCAGC CTGGAGCTGC AGGCAAAGGG AGGAATCCTA CAGAAAAGAT CATACTGCAG
    GTTGACTTCC CCAGTCCAGC GGTGGACGTG CTCTATGTTG GTCCTCAGGA GAACGGCTGC
    CCCAATCCTC AGTCTCTAGA CCCACTTGAT CAAGACGATC GAAGTGAGAT AGAAAAGCTG
    TGCGCACGAG CATCTACTTT CGGACTGTCA CGGGCGGACA GACAGCTGCT TTGGGACCAG
    AGGTACTATT GTCGGGAATA TGAATACAGT TTGCCGAAAA TCCTGGCCAG CGCTCCCAGC
    TGGGACTGGG GCAGTATGGG GGAAATCCAC GCACTTCTGC ACCACTGGCC TGCTCTGTCA
    CCCGTTTCAG CTCTTGAGCT CCTTGAATCC AAGTTTGCCG ACACAGAGGT GAGGAAAGTG
    GCTGTGAGCT GGATCCAGAG CAGCAGTGAT GATGAGCTGG CTGATTATCT CCCCCAGCTA
    GTGCAGGCAG TGAAGTTCGA GTGTCACCTT AACAATGCCT TGGTGAAGTT TCTGCTGTCT
    CGAGCTCTGG GAAATGTCAA CATTGCACAT TATCTTTACT GGCTGCTGAG GGATGCAGTG
    CAGGACCCGG CGTTTGGTCA GCGTTATGAG CGGATCCTCT GTGTGCTGCT GTGTCTGTGT
    GGGACCGGAC TGAGGGCTGA GCTGGAGAAG CAGACCCGAC TTGTGCAGCT GCTTGGAGCT
    CTGGCTGAAA AGGTTCGGCA GGCGAGCAGC TCAACCCGAC AGGTGGTTCT CCTCGAGGGT
    CTAGAGAGGG TTCAGTCTTT CTTCCAGAAG AACAGCTGTC GACTCCCTAT TAGCCCCAGT
    CTGGTGGCAA AAGAGCTCAA TATCAAGGTC TGCTCCTTTT TCAATTCCAA CGCTGTACCT
    CTAAAGATCG CACTGGTTAA CGCAGATCCG CTGGGAGATG AGATTAACGT CATGTTTAAG
    GTTGGAGAAG ACCTGCGGCA GGACATGTTG GCACTACAGA TGATTCGGAT TATGGATCGG
    ATCTGGCTTC AGGAAGGACT CGACCTGCGT ATTGTTAACT TTAAATGCAT CTCTACTGGG
    AAAGATAAAG GTATGTTCCA CTCTACCAAA TTTACATTTG ACAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330105.1
    CDS308..3895
    Translation

    Target ORF information:

    RefSeq Version XM_021474430.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha (pik3c2a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474430.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCCACA TATCCAGTGG CAATGGGTTT AAGTTTGACA GGCCAGCCTC TCCGGGGGTG 
    GTCCGGCCAA AAGGAATTGT GGGGAAGGAA GAGGCTTTAC GCATGGAGGT AGAGGCCTTC
    GATAAAATTA AGCGCGAGAA AAGGCACACA CTCCCAGTCA CAGCCTCTTC TGTTAATCCC
    CTTCCCAATC CAACAGCGCA AACATCCAGC AGCCGGCCAG AGAAAGACCT TATTGTATTC
    TGCGAATCAG AAACACAGAA GAAGGAGCAA AAAGACAACT TTGAGGACAT CGATTTGGAG
    AACCTGACAA AGGAGGAGCT GGAAAAGCTG CTTCTGGATG ACAGTTTTGG TGCAAACAAA
    ATGACAAGAC CTTCCTCTTT GCTAGGTTGC AATCTCAGCG CATCATATCC TGGCGGACAT
    GCCTTCAACC CCTCTTCGTT CCACTGGACA CCCACTCCAA CGCATGCACA GACTCCCATC
    TTCCCCTCTG CTCCATTCGC CAAGCCTCCT TGCTCTTTCC AGAATGGCTT TAGTCCAGCA
    ATGTCTCCAT TTATTCCACC GACCCCCTTT CTCTCCTTTA CTCCCATCCA ACCACCTGCT
    GCTCTGGTCT ACTCGCAGCC TGCCGTCACC CCAGAGATGG CCAAGCTGTT TGACAAGATT
    GCCAGCACTT CGGAATACTT GAAGAACGGC AGGTCCTCTA GCATGGAAAC CGAATCGGCA
    AGTGTCAAGT CTCTGGAGCC CCTACCTCTG CCCTCAGAAC CTCCTAACAT CAGCCGATTT
    GAATGGCTGG ACTTGGACCC TCTCAACAAG CGCAAGGAGG TGGAGGTCGA GGAGACACCT
    GCTGTTTCAA GCTGTCCTTT TATTGAGGAG TCTGGGAAAG CTAAAGACCC GTGGGATGCG
    GTTCTTCAGG ATGAGCATGA AACTGTAGAT AATGGCAGTC CCACCGCAGA GGAAAAGAGC
    AAGGCAACTC AACCCAGAAG AGCATCCACA GGGGCGGCTG TAACAAAAAG CCAATCCCTC
    AGTATATCTG CAAACTCAAC GAATCAAAGC ACAGGCAAAC AGGTCAATAA GGGAAGCACA
    AATGTACTGT CCAAGTACTC TGCCTTACAG GAGAAGGAAG CCCAGAATCT AGAAGTTGTA
    GCATTCTGTG AAGACATTTC AAAACTGAGG TCAAAGTTCC CACACGATGA CCTGTCTACC
    AACCCTGGCT ATGTTCTCAG CCCTGTGATC ACGCAGAGGG ATGCAGGAGG GGACAATGGG
    GGCAGTGTGA AAGTGTCCAT TGAGATTTCT GATTCTCAAC AGCCTGTAAC GTTTACTTGC
    GATGTGACTT CTCCAGTGGA CTTGCTCATA ATGCAGGCTT TGTGTTGGGT TCATGATGAC
    CTGAACCAGG TGGACATCAA TAGCTACGTT CTCAAGGTCT GCGGACAGGA GGAGGTCCTT
    CAGAATAAAC ACAGTCTAGG CAGTCATGAG TACGTGCAGA ACTGCAGGAA GTGGGAATCA
    GAGATCAAAC TACAGCTCCT GTTTCTTAGC ACAATGAGAA GAGATCTGGC CCGAACTGCA
    GAAGATGACA TCTCGCCCAT TGATTTGGAG AAATACCTTG GTCTAGTGGA GAGGCCATTT
    AAAGAGGCAG TCACAAGAGA AGGCCTTGCT GAATATCTAG AAGGCTTTCA TAAGCAAGTC
    AATCTCTGCC TTCAGAATGA GAACAACCAA TATAAAACAG TGGACAATGT GGTTCAGTCT
    GTTAAGAATC TGTGCTGTGC TCTGGATGAA GTTGAGACAC CAGCCATCAC TGATGCCGTC
    AAGAGGCTCA AGCGCTCTGT CAACCTTCCT AGAACACGCT CACCAGAGCA CTATGCTGAA
    TTGCAGGCTG GTGCCACATC TTCCGGAGGT TCAGCTAATG GTTACTCTAG CCCTGTAGAG
    GAGAGTTTAG CAGTGCTCAC AGATGCTGTC TATGAACTCG CCAAGCTCTA CCTTCAGTCT
    TTCTGCCCGA TTAGCCCTGG TTTCATGATA GAAGAGCACA AAGAAGATGA GAGGGACAGC
    AAAGAGGCCT CGGGCACCAC TGAACACTTA CAGTTCACAC TGTTTGCCGT TCATGGCATT
    CCTGCTACCT GGGTCAGCAG TTTTGAAAAA TACTACCTGA TGTGTGCCCT GACCCATAAC
    AACAGGAATC TCTTCAAACC TGTCCAGTCC AAAAAAGTGG GAACATACAA GAGTTTCTTC
    TACCACATCA AATGGGATGA ATTAATAAAC TTCCCTATTT CAGTGTCGCT GTTACCACTG
    GAGGCTATGC TTAGTCTTTC TCTCTATGGG GTCCTCAGTC AGAATGCAAA CAACTCACCA
    GATTCCAACA AGCAACGGAA AGGTCCAGAA CTTTTGGGAA AAGTTTCCAT GCCTCTGTTT
    GACTTCAGGA GGGTTCTGTC CAGAGGCAGT AAACTGCTGA GTTTGTGGAC TTCTCCTCAA
    GCCCTTCAGC CTGGAGCTGC AGGCAAAGGG AGGAATCCTA CAGAAAAGAT CATACTGCAG
    GTTGACTTCC CCAGTCCAGC GGTGGACGTG CTCTATGTTG GTCCTCAGGA GAACGGCTGC
    CCCAATCCTC AGTCTCTAGA CCCACTTGAT CAAGACGATC GAAGTGAGAT AGAAAAGCTG
    TGCGCACGAG CATCTACTTT CGGACTGTCA CGGGCGGACA GACAGCTGCT TTGGGACCAG
    AGGTACTATT GTCGGGAATA TGAATACAGT TTGCCGAAAA TCCTGGCCAG CGCTCCCAGC
    TGGGACTGGG GCAGTATGGG GGAAATCCAC GCACTTCTGC ACCACTGGCC TGCTCTGTCA
    CCCGTTTCAG CTCTTGAGCT CCTTGAATCC AAGTTTGCCG ACACAGAGGT GAGGAAAGTG
    GCTGTGAGCT GGATCCAGAG CAGCAGTGAT GATGAGCTGG CTGATTATCT CCCCCAGCTA
    GTGCAGGCAG TGAAGTTCGA GTGTCACCTT AACAATGCCT TGGTGAAGTT TCTGCTGTCT
    CGAGCTCTGG GAAATGTCAA CATTGCACAT TATCTTTACT GGCTGCTGAG GGATGCAGTG
    CAGGACCCGG CGTTTGGTCA GCGTTATGAG CGGATCCTCT GTGTGCTGCT GTGTCTGTGT
    GGGACCGGAC TGAGGGCTGA GCTGGAGAAG CAGACCCGAC TTGTGCAGCT GCTTGGAGCT
    CTGGCTGAAA AGGTTCGGCA GGCGAGCAGC TCAACCCGAC AGGTGGTTCT CCTCGAGGGT
    CTAGAGAGGG TTCAGTCTTT CTTCCAGAAG AACAGCTGTC GACTCCCTAT TAGCCCCAGT
    CTGGTGGCAA AAGAGCTCAA TATCAAGGTC TGCTCCTTTT TCAATTCCAA CGCTGTACCT
    CTAAAGATCG CACTGGTTAA CGCAGATCCG CTGGGAGATG AGATTAACGT CATGTTTAAG
    GTTGGAGAAG ACCTGCGGCA GGACATGTTG GCACTACAGA TGATTCGGAT TATGGATCGG
    ATCTGGCTTC AGGAAGGACT CGACCTGCGT ATTGTTAACT TTAAATGCAT CTCTACTGGG
    AAAGATAAAG GTATGTTCCA CTCTACCAAA TTTACATTTG ACAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146754
    Clone ID Related Accession (Same CDS sequence) XM_021474431.1
    Accession Version XM_021474431.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3570bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395342.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCCACA TATCCAGTGG CAATGGGTTT AAGTTTGACA GGCCAGCCTC TCCGGGGGTG 
    GTCCGGCCAA AAGGAATTGT GGGGAAGGAA GAGGCTTTAC GCATGGAGGT AGAGGCCTTC
    GATAAAATTA AGCGCGAGAA AAGGCACACA CTCCCAGTCA CAGCCTCTTC TGTTAATCCC
    CTTCCCAATC CAACAGCGCA AACATCCAGC AGCCGGCCAG AGAAAGACCT TATTGTATTC
    TGCGAATCAG AAACACAGAA GAAGGAGCAA AAAGACAACT TTGAGGACAT CGATTTGGAG
    AACCTGACAA AGGAGGAGCT GGAAAAGCTG CTTCTGGATG ACAGTTTTGG TGCAAACAAA
    ATGACAAGAC CTTCCTCTTT GCTAGGTTGC AATCTCAGCG CATCATATCC TGGCGGACAT
    GCCTTCAACC CCTCTTCGTT CCACTGGACA CCCACTCCAA CGCATGCACA GACTCCCATC
    TTCCCCTCTG CTCCATTCGC CAAGCCTCCT TGCTCTTTCC AGAATGGCTT TAGTCCAGCA
    ATGTCTCCAT TTATTCCACC GACCCCCTTT CTCTCCTTTA CTCCCATCCA ACCACCTGCT
    GCTCTGGTCT ACTCGCAGCC TGCCGTCACC CCAGAGATGG CCAAGCTGTT TGACAAGATT
    GCCAGCACTT CGGAATACTT GAAGAACGGC AGGTCCTCTA GCATGGAAAC CGAATCGGCA
    AGTGTCAAGT CTCTGGAGCC CCTACCTCTG CCCTCAGAAC CTCCTAACAT CAGCCGATTT
    GAATGGCTGG ACTTGGACCC TCTCAACAAG CGCAAGGAGG TGGAGGTCGA GGAGACACCT
    GCTGTTTCAA GCTGTCCTTT TATTGAGGAG TCTGGGAAAG CTAAAGACCC GTGGGATGCG
    GTTCTTCAGG ATGAGCATGA AACTGTAGAT AATGGCAGTC CCACCGCAGA GGAAAAGAGC
    AAGGCAACTC AACCCAGAAG AGCATCCACA GGGGCGGCTG TAACAAAAAG CCAATCCCTC
    AGTATATCTG CAAACTCAAC GAATCAAAGC ACAGGCAAAC AGGTCAATAA GGGAAGCACA
    AATGTACTGT CCAAGTACTC TGCCTTACAG GAGAAGGAAG CCCAGAATCT AGAAGTTGTA
    GCATTCTGTG AAGACATTTC AAAACTGAGG TCAAAGTTCC CACACGATGA CCTGTCTACC
    AACCCTGGCT ATGTTCTCAG CCCTGTGATC ACGCAGAGGG ATGCAGGAGG GGACAATGGG
    GGCAGTGTGA AAGTGTCCAT TGAGATTTCT GATTCTCAAC AGCCTGTAAC GTTTACTTGC
    GATGTGACTT CTCCAGTGGA CTTGCTCATA ATGCAGGCTT TGTGTTGGGT TCATGATGAC
    CTGAACCAGG TGGACATCAA TAGCTACGTT CTCAAGGTCT GCGGACAGGA GGAGGTCCTT
    CAGAATAAAC ACAGTCTAGG CAGTCATGAG TACGTGCAGA ACTGCAGGAA GTGGGAATCA
    GAGATCAAAC TACAGCTCCT GTTTCTTAGC ACAATGAGAA GAGATCTGGC CCGAACTGCA
    GAAGATGACA TCTCGCCCAT TGATTTGGAG AAATACCTTG GTCTAGTGGA GAGGCCATTT
    AAAGAGGCAG TCACAAGAGA AGGCCTTGCT GAATATCTAG AAGGCTTTCA TAAGCAAGTC
    AATCTCTGCC TTCAGAATGA GAACAACCAA TATAAAACAG TGGACAATGT GGTTCAGTCT
    GTTAAGAATC TGTGCTGTGC TCTGGATGAA GTTGAGACAC CAGCCATCAC TGATGCCGTC
    AAGAGGCTCA AGCGCTCTGT CAACCTTCCT AGAACACGCT CACCAGAGGC TGGTGCCACA
    TCTTCCGGAG GTTCAGCTAA TGGTTACTCT AGCCCTGTAG AGGAGAGTTT AGCAGTGCTC
    ACAGATGCTG TCTATGAACT CGCCAAGCTC TACCTTCAGT CTTTCTGCCC GATTAGCCCT
    GGTTTCATGA TAGAAGAGCA CAAAGAAGAT GAGAGGGACA GCAAAGAGGC CTCGGGCACC
    ACTGAACACT TACAGTTCAC ACTGTTTGCC GTTCATGGCA TTCCTGCTAC CTGGGTCAGC
    AGTTTTGAAA AATACTACCT GATGTGTGCC CTGACCCATA ACAACAGGAA TCTCTTCAAA
    CCTGTCCAGT CCAAAAAAGT GGGAACATAC AAGAGTTTCT TCTACCACAT CAAATGGGAT
    GAATTAATAA ACTTCCCTAT TTCAGTGTCG CTGTTACCAC TGGAGGCTAT GCTTAGTCTT
    TCTCTCTATG GGGTCCTCAG TCAGAATGCA AACAACTCAC CAGATTCCAA CAAGCAACGG
    AAAGGTCCAG AACTTTTGGG AAAAGTTTCC ATGCCTCTGT TTGACTTCAG GAGGGTTCTG
    TCCAGAGGCA GTAAACTGCT GAGTTTGTGG ACTTCTCCTC AAGCCCTTCA GCCTGGAGCT
    GCAGGCAAAG GGAGGAATCC TACAGAAAAG ATCATACTGC AGGTTGACTT CCCCAGTCCA
    GCGGTGGACG TGCTCTATGT TGGTCCTCAG GAGAACGGCT GCCCCAATCC TCAGTCTCTA
    GACCCACTTG ATCAAGACGA TCGAAGTGAG ATAGAAAAGC TGTGCGCACG AGCATCTACT
    TTCGGACTGT CACGGGCGGA CAGACAGCTG CTTTGGGACC AGAGGTACTA TTGTCGGGAA
    TATGAATACA GTTTGCCGAA AATCCTGGCC AGCGCTCCCA GCTGGGACTG GGGCAGTATG
    GGGGAAATCC ACGCACTTCT GCACCACTGG CCTGCTCTGT CACCCGTTTC AGCTCTTGAG
    CTCCTTGAAT CCAAGTTTGC CGACACAGAG GTGAGGAAAG TGGCTGTGAG CTGGATCCAG
    AGCAGCAGTG ATGATGAGCT GGCTGATTAT CTCCCCCAGC TAGTGCAGGC AGTGAAGTTC
    GAGTGTCACC TTAACAATGC CTTGGTGAAG TTTCTGCTGT CTCGAGCTCT GGGAAATGTC
    AACATTGCAC ATTATCTTTA CTGGCTGCTG AGGGATGCAG TGCAGGACCC GGCGTTTGGT
    CAGCGTTATG AGCGGATCCT CTGTGTGCTG CTGTGTCTGT GTGGGACCGG ACTGAGGGCT
    GAGCTGGAGA AGCAGACCCG ACTTGTGCAG CTGCTTGGAG CTCTGGCTGA AAAGGTTCGG
    CAGGCGAGCA GCTCAACCCG ACAGGTGGTT CTCCTCGAGG GTCTAGAGAG GGTTCAGTCT
    TTCTTCCAGA AGAACAGCTG TCGACTCCCT ATTAGCCCCA GTCTGGTGGC AAAAGAGCTC
    AATATCAAGG TCTGCTCCTT TTTCAATTCC AACGCTGTAC CTCTAAAGAT CGCACTGGTT
    AACGCAGATC CGCTGGGAGA TGAGATTAAC GTCATGTTTA AGGTTGGAGA AGACCTGCGG
    CAGGACATGT TGGCACTACA GATGATTCGG ATTATGGATC GGATCTGGCT TCAGGAAGGA
    CTCGACCTGC GTATTGTTAA CTTTAAATGC ATCTCTACTG GGAAAGATAA AGGTATGTTC
    CACTCTACCA AATTTACATT TGACAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330106.1
    CDS177..3746
    Translation

    Target ORF information:

    RefSeq Version XM_021474431.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha (pik3c2a), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474431.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGCCCACA TATCCAGTGG CAATGGGTTT AAGTTTGACA GGCCAGCCTC TCCGGGGGTG 
    GTCCGGCCAA AAGGAATTGT GGGGAAGGAA GAGGCTTTAC GCATGGAGGT AGAGGCCTTC
    GATAAAATTA AGCGCGAGAA AAGGCACACA CTCCCAGTCA CAGCCTCTTC TGTTAATCCC
    CTTCCCAATC CAACAGCGCA AACATCCAGC AGCCGGCCAG AGAAAGACCT TATTGTATTC
    TGCGAATCAG AAACACAGAA GAAGGAGCAA AAAGACAACT TTGAGGACAT CGATTTGGAG
    AACCTGACAA AGGAGGAGCT GGAAAAGCTG CTTCTGGATG ACAGTTTTGG TGCAAACAAA
    ATGACAAGAC CTTCCTCTTT GCTAGGTTGC AATCTCAGCG CATCATATCC TGGCGGACAT
    GCCTTCAACC CCTCTTCGTT CCACTGGACA CCCACTCCAA CGCATGCACA GACTCCCATC
    TTCCCCTCTG CTCCATTCGC CAAGCCTCCT TGCTCTTTCC AGAATGGCTT TAGTCCAGCA
    ATGTCTCCAT TTATTCCACC GACCCCCTTT CTCTCCTTTA CTCCCATCCA ACCACCTGCT
    GCTCTGGTCT ACTCGCAGCC TGCCGTCACC CCAGAGATGG CCAAGCTGTT TGACAAGATT
    GCCAGCACTT CGGAATACTT GAAGAACGGC AGGTCCTCTA GCATGGAAAC CGAATCGGCA
    AGTGTCAAGT CTCTGGAGCC CCTACCTCTG CCCTCAGAAC CTCCTAACAT CAGCCGATTT
    GAATGGCTGG ACTTGGACCC TCTCAACAAG CGCAAGGAGG TGGAGGTCGA GGAGACACCT
    GCTGTTTCAA GCTGTCCTTT TATTGAGGAG TCTGGGAAAG CTAAAGACCC GTGGGATGCG
    GTTCTTCAGG ATGAGCATGA AACTGTAGAT AATGGCAGTC CCACCGCAGA GGAAAAGAGC
    AAGGCAACTC AACCCAGAAG AGCATCCACA GGGGCGGCTG TAACAAAAAG CCAATCCCTC
    AGTATATCTG CAAACTCAAC GAATCAAAGC ACAGGCAAAC AGGTCAATAA GGGAAGCACA
    AATGTACTGT CCAAGTACTC TGCCTTACAG GAGAAGGAAG CCCAGAATCT AGAAGTTGTA
    GCATTCTGTG AAGACATTTC AAAACTGAGG TCAAAGTTCC CACACGATGA CCTGTCTACC
    AACCCTGGCT ATGTTCTCAG CCCTGTGATC ACGCAGAGGG ATGCAGGAGG GGACAATGGG
    GGCAGTGTGA AAGTGTCCAT TGAGATTTCT GATTCTCAAC AGCCTGTAAC GTTTACTTGC
    GATGTGACTT CTCCAGTGGA CTTGCTCATA ATGCAGGCTT TGTGTTGGGT TCATGATGAC
    CTGAACCAGG TGGACATCAA TAGCTACGTT CTCAAGGTCT GCGGACAGGA GGAGGTCCTT
    CAGAATAAAC ACAGTCTAGG CAGTCATGAG TACGTGCAGA ACTGCAGGAA GTGGGAATCA
    GAGATCAAAC TACAGCTCCT GTTTCTTAGC ACAATGAGAA GAGATCTGGC CCGAACTGCA
    GAAGATGACA TCTCGCCCAT TGATTTGGAG AAATACCTTG GTCTAGTGGA GAGGCCATTT
    AAAGAGGCAG TCACAAGAGA AGGCCTTGCT GAATATCTAG AAGGCTTTCA TAAGCAAGTC
    AATCTCTGCC TTCAGAATGA GAACAACCAA TATAAAACAG TGGACAATGT GGTTCAGTCT
    GTTAAGAATC TGTGCTGTGC TCTGGATGAA GTTGAGACAC CAGCCATCAC TGATGCCGTC
    AAGAGGCTCA AGCGCTCTGT CAACCTTCCT AGAACACGCT CACCAGAGGC TGGTGCCACA
    TCTTCCGGAG GTTCAGCTAA TGGTTACTCT AGCCCTGTAG AGGAGAGTTT AGCAGTGCTC
    ACAGATGCTG TCTATGAACT CGCCAAGCTC TACCTTCAGT CTTTCTGCCC GATTAGCCCT
    GGTTTCATGA TAGAAGAGCA CAAAGAAGAT GAGAGGGACA GCAAAGAGGC CTCGGGCACC
    ACTGAACACT TACAGTTCAC ACTGTTTGCC GTTCATGGCA TTCCTGCTAC CTGGGTCAGC
    AGTTTTGAAA AATACTACCT GATGTGTGCC CTGACCCATA ACAACAGGAA TCTCTTCAAA
    CCTGTCCAGT CCAAAAAAGT GGGAACATAC AAGAGTTTCT TCTACCACAT CAAATGGGAT
    GAATTAATAA ACTTCCCTAT TTCAGTGTCG CTGTTACCAC TGGAGGCTAT GCTTAGTCTT
    TCTCTCTATG GGGTCCTCAG TCAGAATGCA AACAACTCAC CAGATTCCAA CAAGCAACGG
    AAAGGTCCAG AACTTTTGGG AAAAGTTTCC ATGCCTCTGT TTGACTTCAG GAGGGTTCTG
    TCCAGAGGCA GTAAACTGCT GAGTTTGTGG ACTTCTCCTC AAGCCCTTCA GCCTGGAGCT
    GCAGGCAAAG GGAGGAATCC TACAGAAAAG ATCATACTGC AGGTTGACTT CCCCAGTCCA
    GCGGTGGACG TGCTCTATGT TGGTCCTCAG GAGAACGGCT GCCCCAATCC TCAGTCTCTA
    GACCCACTTG ATCAAGACGA TCGAAGTGAG ATAGAAAAGC TGTGCGCACG AGCATCTACT
    TTCGGACTGT CACGGGCGGA CAGACAGCTG CTTTGGGACC AGAGGTACTA TTGTCGGGAA
    TATGAATACA GTTTGCCGAA AATCCTGGCC AGCGCTCCCA GCTGGGACTG GGGCAGTATG
    GGGGAAATCC ACGCACTTCT GCACCACTGG CCTGCTCTGT CACCCGTTTC AGCTCTTGAG
    CTCCTTGAAT CCAAGTTTGC CGACACAGAG GTGAGGAAAG TGGCTGTGAG CTGGATCCAG
    AGCAGCAGTG ATGATGAGCT GGCTGATTAT CTCCCCCAGC TAGTGCAGGC AGTGAAGTTC
    GAGTGTCACC TTAACAATGC CTTGGTGAAG TTTCTGCTGT CTCGAGCTCT GGGAAATGTC
    AACATTGCAC ATTATCTTTA CTGGCTGCTG AGGGATGCAG TGCAGGACCC GGCGTTTGGT
    CAGCGTTATG AGCGGATCCT CTGTGTGCTG CTGTGTCTGT GTGGGACCGG ACTGAGGGCT
    GAGCTGGAGA AGCAGACCCG ACTTGTGCAG CTGCTTGGAG CTCTGGCTGA AAAGGTTCGG
    CAGGCGAGCA GCTCAACCCG ACAGGTGGTT CTCCTCGAGG GTCTAGAGAG GGTTCAGTCT
    TTCTTCCAGA AGAACAGCTG TCGACTCCCT ATTAGCCCCA GTCTGGTGGC AAAAGAGCTC
    AATATCAAGG TCTGCTCCTT TTTCAATTCC AACGCTGTAC CTCTAAAGAT CGCACTGGTT
    AACGCAGATC CGCTGGGAGA TGAGATTAAC GTCATGTTTA AGGTTGGAGA AGACCTGCGG
    CAGGACATGT TGGCACTACA GATGATTCGG ATTATGGATC GGATCTGGCT TCAGGAAGGA
    CTCGACCTGC GTATTGTTAA CTTTAAATGC ATCTCTACTG GGAAAGATAA AGGTATGTTC
    CACTCTACCA AATTTACATT TGACAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146022
    Clone ID Related Accession (Same CDS sequence) XM_005159305.4 , XM_005159306.3
    Accession Version XM_005159305.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395030.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159305.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCCCACA TATCCAGTGG CAATGGGTTT AAGTTTGACA GGCCAGCCTC TCCGGGGGTG 
    GTCCGGCCAA AAGGAATTGT GGGGAAGGAA GAGGCTTTAC GCATGGAGGT AGAGGCCTTC
    GATAAAATTA AGCGCGAGAA AAGGCACACA CTCCCAGTCA CAGCCTCTTC TGTTAATCCC
    CTTCCCAATC CAACAGCGCA AACATCCACC AGCCGGCCAG AGAAAGACCT TATTGTATTC
    TGCGAATCAG AAACACAGAA GAAGGAGCAA AAAGACAACT TTGAGGACAT CGATTTGGAG
    AACCTGACAA AGGAGGAGCT GGAAAAGCTG CTTCTGGATG ACAGTTTTGG TGCAAACAAA
    ATGACAAGAC CTTCCTCTTT GCTAGGTTGC AATCTCAGCG CATCATATCC TGGCGGACAT
    GCCTTCAACC CCTCTTCGTT CCACTGGACA CCCACTCCAA CGCATGCACA GACTCCCATC
    TTCCCCTCTG CTCCATTCGC CAAGCCTCCT TGCTCTTTCC AGAATGGCTT TAGTCCAGCA
    ATGTCTCCAT TTATTCCACC GACCCCCTTT CTCTCCTTTA CTCCCATCCA ACCACCTGCT
    GCTCTGGTCT ACTCACAGCC TGCCGTCACC CCAGAGATGG CCAAGCTGTT TGACAAGATT
    GCCAGCACTT CGGAATACTT GAAGAACGGC AGGTCCTCTA GCATGGAAAC CGAATCGGCA
    AGTGTCAAGT CTCTGGAGCC CCTACCTCAG CCCTCAGAAC CTCCTAACAT CAGCCGATTT
    GAATGGCTGG ACTTGGACCC TCTCAACAAG CGCAAGGAGG TGGAGGTCGA GGAGACACCT
    GCTGTTTCAA GCTGTCCTTT TATTGAGGAG TCTGGGAAAG CTAAAGACCC GTGGGATGCG
    GTTCTTCAGG ATGAGCATGA AACTGTAGAT AATGGCAGTC CCAAAGCAGA GGAAAAGTGC
    AAGGCAACTC AACCCAGAAG AGCATCCACA GGGGCGGCTG TAACAAAAAG CCAATCCCTC
    AGTATATCTT CAAACTCAAC GAATCAAAGC ACAGGCAAAC AGGTCAATAA GGGAAGCACA
    AATGTACTGT CCAAGTACTC TGCCTTACAG GAGAAGGAAG CCCAGAATCT AGAAGTTGTA
    GCATTCTGTG AAGACATTTC AAAACTGAGG TCAAAGTTCC CACACGATGA CCTGTCTACC
    AACCCTGGCT ATGTTCTCAG CCCTGTGATC ACGCAGAGGG ATGCAGGAGG GGACAATGGG
    GGCAGTGTGA AAGTGTCCAT TGAGATTTCT GATTCTCAAC AGCCTGTAAC GTTTACTTGC
    GATGTGACTT CTCCAGTGGA CTTGCTCATA ATGCAGGCTT TGTGTTGGGT TCATGATGAC
    CTGAACCAGG TGGACATCAA TAGCTACGTT CTCAAGGTCT GCGGACAGGA GGAGGTCCTT
    CAGAATAAAC ACAGTCTAGG CAGTCATGAG TACGTGCAGA ACTGCAGGAA GTGGGAATCA
    GAGATCAAAC TACAGCTCCT GTTTCTTAGC ACAATGAGAA GAGATCTGGC CCGAACTGCA
    GAAGATGACA TCTCGCCCAT TGATTTGGAG AAATACCTTG GTCTAGTGGA GAGGCCATTT
    AAAGAGGCAG TCACAAGAGA AGGCCTTGCT GAATATCTAG AAGGCTTTCA TAAGCAAGTC
    AATCTCTGCC TTCAGAATGA GAACAACCAA TATAAAACAG TGGACAATGT GGTTCAGTCT
    GTTAAGAATC TGTGCTGTGC TCTGGATGAA GTTGAGACAC CAGCCATCAC TGATGCCGTC
    AAGAGGCTCA AGCGCTCTGT CAACCTTCCT AGAACACGCT CACCAGAGCA CTATGCTGAA
    TTGCAGGCTG GTGCCACATC TTCCGGAGGT TCAGCTAATG GTTACTCTAG CCCTGTAGAG
    GAGAGTTTAG CAGTGCTCAC AGATGCTGTC TATGAACTCG CCAAGCTCTA CCTTCAGTCT
    TTCTGCCCGA TTAGCCCTGG TTTCATGATA GAAGAGCACA AAGAAGATGA GAGGGACAGC
    AAAGAGGCCT CGGGCACCAC TGAACACTTA CAGTTCACAC TGTTTGCCGT TCATGGCATT
    CCTGCTACCT GGGTCAGCAG TTTTGAAAAA TACTACCTGA TGTGTGCCCT GACCCATAAC
    AACAGGAATC TCTTCAAACC TGTCCAGTCC AAAAAAGTGG GAACATACAA GAGTTTCTTC
    TACCACATCA AATGGGATGA ATTAATAAAC TTCCCTATTT CAGTGTCGCT GTTACCACTG
    GAGGCTATGC TTAGTCTTTC TCTCTATGGG GTCCTCAGTC AGAATGCAAA CAACTCACCA
    GATTCCAACA AGCAACGGAA AGGTCCAGAA CTTTTGGGAA AAGTTTCCAT GCCTCTGTTT
    GACTTCAGGA GGGTTCTGTC CAGAGGCAGT AAACTGCTGA GTTTGTGGAC TTCTCCTCAA
    GCCCTTCAGC CTGGAGCTGC AGGCAAAGGG AGGAATCCTA CAGAAAAGAT CATACTGCAG
    GTTGACTTCC CCAGTCCAGC GGTGGACGTG CTCTATGTTG GTCCTCAGGA GAACGGTTGC
    CCCAATCCTC AGTCTCTAGA CCCACTTGAT CAAGACGATC GAAGTGAGAT AGAAAAGCTG
    TGCGCACGAG CATCTACTTT CGGACTGTCA CGGGCGGACA GACAGCTGCT TTGGGACCAG
    AGGTACTATT GTCGGGAATA TGAATACAGT TTGCCGAAAA TCCTGGCCAG CGCTCCCAGC
    TGGGACTGGG GCAGTATGGG GGAAATCCAC GCACTTCTGC ACCACTGGCC TGCTCTGTCA
    CCCGTTTCAG CTCTTGAGCT CCTTGAATCC AAGTTTGCCG ACACAGAGGT GAGGAAAGTG
    GCTGTGAGCT GGATCCAGAG CAGCAGTGAT GATGAGCTGG CTGATTATCT CCCCCAGTTA
    GTGCAGGCAG TGAAGTTCGA GTGTCACCTC AAAAATGCCT TGGTGAAGTT TCTGCTGTCT
    CGAGCTCTGG GAAATGTCAA CATTGCACAT TATCTTTACT GGCTGCTGAG GGATGCAGTG
    CAGGACCCGG CGTTTGGTCA GCGTTATGAG CGGATCCTCT GTGTGCTGCT GTGTCTGTGT
    GGGACCGGAC TAAGGGCTGA GCTGGAGAAG CAGACCCGAC TTGTGCAGCT GCTTGGAGCT
    CTGGCTGAAA AGGTTCGGCA GGCGAGCAGC TCAACCCGAC AGGTGGTTCT CCTCGAGGGT
    CTAGAGAGGG TTCAGTCTTT CTTCCAGAAG AACAGCTGTC GACTCCCTAT TAGCCCCAGT
    CTGGTGGCAA AAGAGCTCAA TATCAAGGTC TGCTCCTTTT TCAATTCCAA CGCTGTACCT
    CTAAAGATCG CACTGGTTAA CGCAGATCCG CTGGGAGATG AGATTAACGT CATGTTTAAG
    GTTGGAGAAG ACCTGCGGCA GGACATGTTG GCACTACAGA TGATTCGGAT TATGGATCGG
    ATCTGGCTTC AGGAAGGACT CGACCTGCGT ATTGTTAACT TTAAATGCAT CTCTACTGGG
    AAAGATAAAG GCATGGTGGA GTTGGTACCA TCTTCTGAAA CACTGAGAAA GATTCAGGTG
    GAGTATGGAG TGACGGGTTC CTTCAAGGAC AAACCTCTTG CTGAGTGGCT TCGCAAGTAT
    AACCCTGCTG AGGATGAGTA TGAGAAGGCG TCTGAGAATT TTATCTACAC CTGTGCTGGA
    TGCTGTGTGG CCACTTACAT CCTCGGCATC TGCGATCGTC ACAACGACAA CATCATGCTG
    CGCTCCACTG GCCACATGTT CCACATAGAC TTCGGGAAGT TCCTGGGTCA CGCACAAATG
    ATTGGGAGTT TTAAAAGGGA CCGAGCACCG TTTGTCTTAA CCTCTGACAT GGCTTATGTC
    ATCAATGGAG GCGAAAGACC CACTAGTCGA TTTCAGCTGT TCGTTGACCT TTGCTCGCAA
    GCCTACAACC TGATCCGCAA ACACTCCAAC CTCTTCCTCA ACCTGCTATC TCTGATGACA
    CAATCAGGTT TACCCGAGCT GACCGGAGTC CAAGATTTGA AGTATGTGTA TGATGCTCTT
    CAGCCTCAGA CTACAGATGC AGAGGCTACC ATTTTCTTCA CAAGGTTGAT TGAATCCAGT
    CTGGGCAGTG TGGCTACAAA GTTTAATTTC TTTATCCACA ATCTGGCTCA GTTGCGTTTC
    TCTGGCCTTC CCTCCAATGA TGAACCCATC CTGTCGTTTG CTCCTCGGAC ATACACTATG
    AAGCAAGACG GCAAGATTCG GGATGCCTCT GTGTTTTCAT ATCAAAAGAG ATATAACCCA
    GACAAGCACT ATACGTATGT CATTCGGATT CTAAGGGAAG GTCAGAGCGA GCCACAGTTT
    GTTTTCCGCA CATTTGATGA GTTTCAGGAA TTGCACAACA AATTGACCAT CCTTTTTCAG
    TTGTGGAAGC TGCCAGGGTT TCCCAGTAAG ATGGTGCTGG GACGCACACA CATCAAGGAT
    GTGGCATCCA AGAGAAAGGT TGAATTGAAT AGTTATGTGC ACAATCTGAT GAGGAGTTCA
    ACAGAGGTCG CCCAGTGTGA TCTGATCTAC ACCTTCTTCC ACCCTATCGC AAGAGATGAC
    AAGACTGAAG GGGTTGATAC CCTATCAAAA ACTCCAGACA TGCCTCCAGT GAGTCCCACC
    ACAGGCCGTG TGGAGGGTGA GGTGAAGCTG TCAGTGTCCT ACAGAAACAG CACACTTTTC
    ATCATGGTCA TGCACATTAA AGACCTGATG TCCAATGACG GAGCAGACCC AAACCCTTAT
    GTGAAAACAT ACCTTCTCCC AGACCCCCAC AAAACTTCCA AACGCAAAAC AAAGATTGCG
    AGGAAGACAA GGAACCCAAC ATTTAATGAA ATGCTGGTGT ATAGCGGTTA CAGTAAGGAA
    ACACTGAAGC AAAGGGAACT TCAACTCAGT GTGCTCAGTG CCGAGTCTCT GCGAGAGAAC
    TGCTATTTGG GAGGAATCAC CCTCTGCCTT AAAGACTTTG ACCTCAGCCG AGAGACAGTC
    AAGTGGTACA AACTCACCAC GGTGCCCTAC TTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005159362.2
    CDS177..5252
    Translation

    Target ORF information:

    RefSeq Version XM_005159305.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha (pik3c2a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005159305.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCCCACA TATCCAGTGG CAATGGGTTT AAGTTTGACA GGCCAGCCTC TCCGGGGGTG 
    GTCCGGCCAA AAGGAATTGT GGGGAAGGAA GAGGCTTTAC GCATGGAGGT AGAGGCCTTC
    GATAAAATTA AGCGCGAGAA AAGGCACACA CTCCCAGTCA CAGCCTCTTC TGTTAATCCC
    CTTCCCAATC CAACAGCGCA AACATCCACC AGCCGGCCAG AGAAAGACCT TATTGTATTC
    TGCGAATCAG AAACACAGAA GAAGGAGCAA AAAGACAACT TTGAGGACAT CGATTTGGAG
    AACCTGACAA AGGAGGAGCT GGAAAAGCTG CTTCTGGATG ACAGTTTTGG TGCAAACAAA
    ATGACAAGAC CTTCCTCTTT GCTAGGTTGC AATCTCAGCG CATCATATCC TGGCGGACAT
    GCCTTCAACC CCTCTTCGTT CCACTGGACA CCCACTCCAA CGCATGCACA GACTCCCATC
    TTCCCCTCTG CTCCATTCGC CAAGCCTCCT TGCTCTTTCC AGAATGGCTT TAGTCCAGCA
    ATGTCTCCAT TTATTCCACC GACCCCCTTT CTCTCCTTTA CTCCCATCCA ACCACCTGCT
    GCTCTGGTCT ACTCACAGCC TGCCGTCACC CCAGAGATGG CCAAGCTGTT TGACAAGATT
    GCCAGCACTT CGGAATACTT GAAGAACGGC AGGTCCTCTA GCATGGAAAC CGAATCGGCA
    AGTGTCAAGT CTCTGGAGCC CCTACCTCAG CCCTCAGAAC CTCCTAACAT CAGCCGATTT
    GAATGGCTGG ACTTGGACCC TCTCAACAAG CGCAAGGAGG TGGAGGTCGA GGAGACACCT
    GCTGTTTCAA GCTGTCCTTT TATTGAGGAG TCTGGGAAAG CTAAAGACCC GTGGGATGCG
    GTTCTTCAGG ATGAGCATGA AACTGTAGAT AATGGCAGTC CCAAAGCAGA GGAAAAGTGC
    AAGGCAACTC AACCCAGAAG AGCATCCACA GGGGCGGCTG TAACAAAAAG CCAATCCCTC
    AGTATATCTT CAAACTCAAC GAATCAAAGC ACAGGCAAAC AGGTCAATAA GGGAAGCACA
    AATGTACTGT CCAAGTACTC TGCCTTACAG GAGAAGGAAG CCCAGAATCT AGAAGTTGTA
    GCATTCTGTG AAGACATTTC AAAACTGAGG TCAAAGTTCC CACACGATGA CCTGTCTACC
    AACCCTGGCT ATGTTCTCAG CCCTGTGATC ACGCAGAGGG ATGCAGGAGG GGACAATGGG
    GGCAGTGTGA AAGTGTCCAT TGAGATTTCT GATTCTCAAC AGCCTGTAAC GTTTACTTGC
    GATGTGACTT CTCCAGTGGA CTTGCTCATA ATGCAGGCTT TGTGTTGGGT TCATGATGAC
    CTGAACCAGG TGGACATCAA TAGCTACGTT CTCAAGGTCT GCGGACAGGA GGAGGTCCTT
    CAGAATAAAC ACAGTCTAGG CAGTCATGAG TACGTGCAGA ACTGCAGGAA GTGGGAATCA
    GAGATCAAAC TACAGCTCCT GTTTCTTAGC ACAATGAGAA GAGATCTGGC CCGAACTGCA
    GAAGATGACA TCTCGCCCAT TGATTTGGAG AAATACCTTG GTCTAGTGGA GAGGCCATTT
    AAAGAGGCAG TCACAAGAGA AGGCCTTGCT GAATATCTAG AAGGCTTTCA TAAGCAAGTC
    AATCTCTGCC TTCAGAATGA GAACAACCAA TATAAAACAG TGGACAATGT GGTTCAGTCT
    GTTAAGAATC TGTGCTGTGC TCTGGATGAA GTTGAGACAC CAGCCATCAC TGATGCCGTC
    AAGAGGCTCA AGCGCTCTGT CAACCTTCCT AGAACACGCT CACCAGAGCA CTATGCTGAA
    TTGCAGGCTG GTGCCACATC TTCCGGAGGT TCAGCTAATG GTTACTCTAG CCCTGTAGAG
    GAGAGTTTAG CAGTGCTCAC AGATGCTGTC TATGAACTCG CCAAGCTCTA CCTTCAGTCT
    TTCTGCCCGA TTAGCCCTGG TTTCATGATA GAAGAGCACA AAGAAGATGA GAGGGACAGC
    AAAGAGGCCT CGGGCACCAC TGAACACTTA CAGTTCACAC TGTTTGCCGT TCATGGCATT
    CCTGCTACCT GGGTCAGCAG TTTTGAAAAA TACTACCTGA TGTGTGCCCT GACCCATAAC
    AACAGGAATC TCTTCAAACC TGTCCAGTCC AAAAAAGTGG GAACATACAA GAGTTTCTTC
    TACCACATCA AATGGGATGA ATTAATAAAC TTCCCTATTT CAGTGTCGCT GTTACCACTG
    GAGGCTATGC TTAGTCTTTC TCTCTATGGG GTCCTCAGTC AGAATGCAAA CAACTCACCA
    GATTCCAACA AGCAACGGAA AGGTCCAGAA CTTTTGGGAA AAGTTTCCAT GCCTCTGTTT
    GACTTCAGGA GGGTTCTGTC CAGAGGCAGT AAACTGCTGA GTTTGTGGAC TTCTCCTCAA
    GCCCTTCAGC CTGGAGCTGC AGGCAAAGGG AGGAATCCTA CAGAAAAGAT CATACTGCAG
    GTTGACTTCC CCAGTCCAGC GGTGGACGTG CTCTATGTTG GTCCTCAGGA GAACGGTTGC
    CCCAATCCTC AGTCTCTAGA CCCACTTGAT CAAGACGATC GAAGTGAGAT AGAAAAGCTG
    TGCGCACGAG CATCTACTTT CGGACTGTCA CGGGCGGACA GACAGCTGCT TTGGGACCAG
    AGGTACTATT GTCGGGAATA TGAATACAGT TTGCCGAAAA TCCTGGCCAG CGCTCCCAGC
    TGGGACTGGG GCAGTATGGG GGAAATCCAC GCACTTCTGC ACCACTGGCC TGCTCTGTCA
    CCCGTTTCAG CTCTTGAGCT CCTTGAATCC AAGTTTGCCG ACACAGAGGT GAGGAAAGTG
    GCTGTGAGCT GGATCCAGAG CAGCAGTGAT GATGAGCTGG CTGATTATCT CCCCCAGTTA
    GTGCAGGCAG TGAAGTTCGA GTGTCACCTC AAAAATGCCT TGGTGAAGTT TCTGCTGTCT
    CGAGCTCTGG GAAATGTCAA CATTGCACAT TATCTTTACT GGCTGCTGAG GGATGCAGTG
    CAGGACCCGG CGTTTGGTCA GCGTTATGAG CGGATCCTCT GTGTGCTGCT GTGTCTGTGT
    GGGACCGGAC TAAGGGCTGA GCTGGAGAAG CAGACCCGAC TTGTGCAGCT GCTTGGAGCT
    CTGGCTGAAA AGGTTCGGCA GGCGAGCAGC TCAACCCGAC AGGTGGTTCT CCTCGAGGGT
    CTAGAGAGGG TTCAGTCTTT CTTCCAGAAG AACAGCTGTC GACTCCCTAT TAGCCCCAGT
    CTGGTGGCAA AAGAGCTCAA TATCAAGGTC TGCTCCTTTT TCAATTCCAA CGCTGTACCT
    CTAAAGATCG CACTGGTTAA CGCAGATCCG CTGGGAGATG AGATTAACGT CATGTTTAAG
    GTTGGAGAAG ACCTGCGGCA GGACATGTTG GCACTACAGA TGATTCGGAT TATGGATCGG
    ATCTGGCTTC AGGAAGGACT CGACCTGCGT ATTGTTAACT TTAAATGCAT CTCTACTGGG
    AAAGATAAAG GCATGGTGGA GTTGGTACCA TCTTCTGAAA CACTGAGAAA GATTCAGGTG
    GAGTATGGAG TGACGGGTTC CTTCAAGGAC AAACCTCTTG CTGAGTGGCT TCGCAAGTAT
    AACCCTGCTG AGGATGAGTA TGAGAAGGCG TCTGAGAATT TTATCTACAC CTGTGCTGGA
    TGCTGTGTGG CCACTTACAT CCTCGGCATC TGCGATCGTC ACAACGACAA CATCATGCTG
    CGCTCCACTG GCCACATGTT CCACATAGAC TTCGGGAAGT TCCTGGGTCA CGCACAAATG
    ATTGGGAGTT TTAAAAGGGA CCGAGCACCG TTTGTCTTAA CCTCTGACAT GGCTTATGTC
    ATCAATGGAG GCGAAAGACC CACTAGTCGA TTTCAGCTGT TCGTTGACCT TTGCTCGCAA
    GCCTACAACC TGATCCGCAA ACACTCCAAC CTCTTCCTCA ACCTGCTATC TCTGATGACA
    CAATCAGGTT TACCCGAGCT GACCGGAGTC CAAGATTTGA AGTATGTGTA TGATGCTCTT
    CAGCCTCAGA CTACAGATGC AGAGGCTACC ATTTTCTTCA CAAGGTTGAT TGAATCCAGT
    CTGGGCAGTG TGGCTACAAA GTTTAATTTC TTTATCCACA ATCTGGCTCA GTTGCGTTTC
    TCTGGCCTTC CCTCCAATGA TGAACCCATC CTGTCGTTTG CTCCTCGGAC ATACACTATG
    AAGCAAGACG GCAAGATTCG GGATGCCTCT GTGTTTTCAT ATCAAAAGAG ATATAACCCA
    GACAAGCACT ATACGTATGT CATTCGGATT CTAAGGGAAG GTCAGAGCGA GCCACAGTTT
    GTTTTCCGCA CATTTGATGA GTTTCAGGAA TTGCACAACA AATTGACCAT CCTTTTTCAG
    TTGTGGAAGC TGCCAGGGTT TCCCAGTAAG ATGGTGCTGG GACGCACACA CATCAAGGAT
    GTGGCATCCA AGAGAAAGGT TGAATTGAAT AGTTATGTGC ACAATCTGAT GAGGAGTTCA
    ACAGAGGTCG CCCAGTGTGA TCTGATCTAC ACCTTCTTCC ACCCTATCGC AAGAGATGAC
    AAGACTGAAG GGGTTGATAC CCTATCAAAA ACTCCAGACA TGCCTCCAGT GAGTCCCACC
    ACAGGCCGTG TGGAGGGTGA GGTGAAGCTG TCAGTGTCCT ACAGAAACAG CACACTTTTC
    ATCATGGTCA TGCACATTAA AGACCTGATG TCCAATGACG GAGCAGACCC AAACCCTTAT
    GTGAAAACAT ACCTTCTCCC AGACCCCCAC AAAACTTCCA AACGCAAAAC AAAGATTGCG
    AGGAAGACAA GGAACCCAAC ATTTAATGAA ATGCTGGTGT ATAGCGGTTA CAGTAAGGAA
    ACACTGAAGC AAAGGGAACT TCAACTCAGT GTGCTCAGTG CCGAGTCTCT GCGAGAGAAC
    TGCTATTTGG GAGGAATCAC CCTCTGCCTT AAAGACTTTG ACCTCAGCCG AGAGACAGTC
    AAGTGGTACA AACTCACCAC GGTGCCCTAC TTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146022
    Clone ID Related Accession (Same CDS sequence) XM_005159305.4 , XM_005159306.3
    Accession Version XM_005159306.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395030.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159306.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCCCACA TATCCAGTGG CAATGGGTTT AAGTTTGACA GGCCAGCCTC TCCGGGGGTG 
    GTCCGGCCAA AAGGAATTGT GGGGAAGGAA GAGGCTTTAC GCATGGAGGT AGAGGCCTTC
    GATAAAATTA AGCGCGAGAA AAGGCACACA CTCCCAGTCA CAGCCTCTTC TGTTAATCCC
    CTTCCCAATC CAACAGCGCA AACATCCACC AGCCGGCCAG AGAAAGACCT TATTGTATTC
    TGCGAATCAG AAACACAGAA GAAGGAGCAA AAAGACAACT TTGAGGACAT CGATTTGGAG
    AACCTGACAA AGGAGGAGCT GGAAAAGCTG CTTCTGGATG ACAGTTTTGG TGCAAACAAA
    ATGACAAGAC CTTCCTCTTT GCTAGGTTGC AATCTCAGCG CATCATATCC TGGCGGACAT
    GCCTTCAACC CCTCTTCGTT CCACTGGACA CCCACTCCAA CGCATGCACA GACTCCCATC
    TTCCCCTCTG CTCCATTCGC CAAGCCTCCT TGCTCTTTCC AGAATGGCTT TAGTCCAGCA
    ATGTCTCCAT TTATTCCACC GACCCCCTTT CTCTCCTTTA CTCCCATCCA ACCACCTGCT
    GCTCTGGTCT ACTCACAGCC TGCCGTCACC CCAGAGATGG CCAAGCTGTT TGACAAGATT
    GCCAGCACTT CGGAATACTT GAAGAACGGC AGGTCCTCTA GCATGGAAAC CGAATCGGCA
    AGTGTCAAGT CTCTGGAGCC CCTACCTCAG CCCTCAGAAC CTCCTAACAT CAGCCGATTT
    GAATGGCTGG ACTTGGACCC TCTCAACAAG CGCAAGGAGG TGGAGGTCGA GGAGACACCT
    GCTGTTTCAA GCTGTCCTTT TATTGAGGAG TCTGGGAAAG CTAAAGACCC GTGGGATGCG
    GTTCTTCAGG ATGAGCATGA AACTGTAGAT AATGGCAGTC CCAAAGCAGA GGAAAAGTGC
    AAGGCAACTC AACCCAGAAG AGCATCCACA GGGGCGGCTG TAACAAAAAG CCAATCCCTC
    AGTATATCTT CAAACTCAAC GAATCAAAGC ACAGGCAAAC AGGTCAATAA GGGAAGCACA
    AATGTACTGT CCAAGTACTC TGCCTTACAG GAGAAGGAAG CCCAGAATCT AGAAGTTGTA
    GCATTCTGTG AAGACATTTC AAAACTGAGG TCAAAGTTCC CACACGATGA CCTGTCTACC
    AACCCTGGCT ATGTTCTCAG CCCTGTGATC ACGCAGAGGG ATGCAGGAGG GGACAATGGG
    GGCAGTGTGA AAGTGTCCAT TGAGATTTCT GATTCTCAAC AGCCTGTAAC GTTTACTTGC
    GATGTGACTT CTCCAGTGGA CTTGCTCATA ATGCAGGCTT TGTGTTGGGT TCATGATGAC
    CTGAACCAGG TGGACATCAA TAGCTACGTT CTCAAGGTCT GCGGACAGGA GGAGGTCCTT
    CAGAATAAAC ACAGTCTAGG CAGTCATGAG TACGTGCAGA ACTGCAGGAA GTGGGAATCA
    GAGATCAAAC TACAGCTCCT GTTTCTTAGC ACAATGAGAA GAGATCTGGC CCGAACTGCA
    GAAGATGACA TCTCGCCCAT TGATTTGGAG AAATACCTTG GTCTAGTGGA GAGGCCATTT
    AAAGAGGCAG TCACAAGAGA AGGCCTTGCT GAATATCTAG AAGGCTTTCA TAAGCAAGTC
    AATCTCTGCC TTCAGAATGA GAACAACCAA TATAAAACAG TGGACAATGT GGTTCAGTCT
    GTTAAGAATC TGTGCTGTGC TCTGGATGAA GTTGAGACAC CAGCCATCAC TGATGCCGTC
    AAGAGGCTCA AGCGCTCTGT CAACCTTCCT AGAACACGCT CACCAGAGCA CTATGCTGAA
    TTGCAGGCTG GTGCCACATC TTCCGGAGGT TCAGCTAATG GTTACTCTAG CCCTGTAGAG
    GAGAGTTTAG CAGTGCTCAC AGATGCTGTC TATGAACTCG CCAAGCTCTA CCTTCAGTCT
    TTCTGCCCGA TTAGCCCTGG TTTCATGATA GAAGAGCACA AAGAAGATGA GAGGGACAGC
    AAAGAGGCCT CGGGCACCAC TGAACACTTA CAGTTCACAC TGTTTGCCGT TCATGGCATT
    CCTGCTACCT GGGTCAGCAG TTTTGAAAAA TACTACCTGA TGTGTGCCCT GACCCATAAC
    AACAGGAATC TCTTCAAACC TGTCCAGTCC AAAAAAGTGG GAACATACAA GAGTTTCTTC
    TACCACATCA AATGGGATGA ATTAATAAAC TTCCCTATTT CAGTGTCGCT GTTACCACTG
    GAGGCTATGC TTAGTCTTTC TCTCTATGGG GTCCTCAGTC AGAATGCAAA CAACTCACCA
    GATTCCAACA AGCAACGGAA AGGTCCAGAA CTTTTGGGAA AAGTTTCCAT GCCTCTGTTT
    GACTTCAGGA GGGTTCTGTC CAGAGGCAGT AAACTGCTGA GTTTGTGGAC TTCTCCTCAA
    GCCCTTCAGC CTGGAGCTGC AGGCAAAGGG AGGAATCCTA CAGAAAAGAT CATACTGCAG
    GTTGACTTCC CCAGTCCAGC GGTGGACGTG CTCTATGTTG GTCCTCAGGA GAACGGTTGC
    CCCAATCCTC AGTCTCTAGA CCCACTTGAT CAAGACGATC GAAGTGAGAT AGAAAAGCTG
    TGCGCACGAG CATCTACTTT CGGACTGTCA CGGGCGGACA GACAGCTGCT TTGGGACCAG
    AGGTACTATT GTCGGGAATA TGAATACAGT TTGCCGAAAA TCCTGGCCAG CGCTCCCAGC
    TGGGACTGGG GCAGTATGGG GGAAATCCAC GCACTTCTGC ACCACTGGCC TGCTCTGTCA
    CCCGTTTCAG CTCTTGAGCT CCTTGAATCC AAGTTTGCCG ACACAGAGGT GAGGAAAGTG
    GCTGTGAGCT GGATCCAGAG CAGCAGTGAT GATGAGCTGG CTGATTATCT CCCCCAGTTA
    GTGCAGGCAG TGAAGTTCGA GTGTCACCTC AAAAATGCCT TGGTGAAGTT TCTGCTGTCT
    CGAGCTCTGG GAAATGTCAA CATTGCACAT TATCTTTACT GGCTGCTGAG GGATGCAGTG
    CAGGACCCGG CGTTTGGTCA GCGTTATGAG CGGATCCTCT GTGTGCTGCT GTGTCTGTGT
    GGGACCGGAC TAAGGGCTGA GCTGGAGAAG CAGACCCGAC TTGTGCAGCT GCTTGGAGCT
    CTGGCTGAAA AGGTTCGGCA GGCGAGCAGC TCAACCCGAC AGGTGGTTCT CCTCGAGGGT
    CTAGAGAGGG TTCAGTCTTT CTTCCAGAAG AACAGCTGTC GACTCCCTAT TAGCCCCAGT
    CTGGTGGCAA AAGAGCTCAA TATCAAGGTC TGCTCCTTTT TCAATTCCAA CGCTGTACCT
    CTAAAGATCG CACTGGTTAA CGCAGATCCG CTGGGAGATG AGATTAACGT CATGTTTAAG
    GTTGGAGAAG ACCTGCGGCA GGACATGTTG GCACTACAGA TGATTCGGAT TATGGATCGG
    ATCTGGCTTC AGGAAGGACT CGACCTGCGT ATTGTTAACT TTAAATGCAT CTCTACTGGG
    AAAGATAAAG GCATGGTGGA GTTGGTACCA TCTTCTGAAA CACTGAGAAA GATTCAGGTG
    GAGTATGGAG TGACGGGTTC CTTCAAGGAC AAACCTCTTG CTGAGTGGCT TCGCAAGTAT
    AACCCTGCTG AGGATGAGTA TGAGAAGGCG TCTGAGAATT TTATCTACAC CTGTGCTGGA
    TGCTGTGTGG CCACTTACAT CCTCGGCATC TGCGATCGTC ACAACGACAA CATCATGCTG
    CGCTCCACTG GCCACATGTT CCACATAGAC TTCGGGAAGT TCCTGGGTCA CGCACAAATG
    ATTGGGAGTT TTAAAAGGGA CCGAGCACCG TTTGTCTTAA CCTCTGACAT GGCTTATGTC
    ATCAATGGAG GCGAAAGACC CACTAGTCGA TTTCAGCTGT TCGTTGACCT TTGCTCGCAA
    GCCTACAACC TGATCCGCAA ACACTCCAAC CTCTTCCTCA ACCTGCTATC TCTGATGACA
    CAATCAGGTT TACCCGAGCT GACCGGAGTC CAAGATTTGA AGTATGTGTA TGATGCTCTT
    CAGCCTCAGA CTACAGATGC AGAGGCTACC ATTTTCTTCA CAAGGTTGAT TGAATCCAGT
    CTGGGCAGTG TGGCTACAAA GTTTAATTTC TTTATCCACA ATCTGGCTCA GTTGCGTTTC
    TCTGGCCTTC CCTCCAATGA TGAACCCATC CTGTCGTTTG CTCCTCGGAC ATACACTATG
    AAGCAAGACG GCAAGATTCG GGATGCCTCT GTGTTTTCAT ATCAAAAGAG ATATAACCCA
    GACAAGCACT ATACGTATGT CATTCGGATT CTAAGGGAAG GTCAGAGCGA GCCACAGTTT
    GTTTTCCGCA CATTTGATGA GTTTCAGGAA TTGCACAACA AATTGACCAT CCTTTTTCAG
    TTGTGGAAGC TGCCAGGGTT TCCCAGTAAG ATGGTGCTGG GACGCACACA CATCAAGGAT
    GTGGCATCCA AGAGAAAGGT TGAATTGAAT AGTTATGTGC ACAATCTGAT GAGGAGTTCA
    ACAGAGGTCG CCCAGTGTGA TCTGATCTAC ACCTTCTTCC ACCCTATCGC AAGAGATGAC
    AAGACTGAAG GGGTTGATAC CCTATCAAAA ACTCCAGACA TGCCTCCAGT GAGTCCCACC
    ACAGGCCGTG TGGAGGGTGA GGTGAAGCTG TCAGTGTCCT ACAGAAACAG CACACTTTTC
    ATCATGGTCA TGCACATTAA AGACCTGATG TCCAATGACG GAGCAGACCC AAACCCTTAT
    GTGAAAACAT ACCTTCTCCC AGACCCCCAC AAAACTTCCA AACGCAAAAC AAAGATTGCG
    AGGAAGACAA GGAACCCAAC ATTTAATGAA ATGCTGGTGT ATAGCGGTTA CAGTAAGGAA
    ACACTGAAGC AAAGGGAACT TCAACTCAGT GTGCTCAGTG CCGAGTCTCT GCGAGAGAAC
    TGCTATTTGG GAGGAATCAC CCTCTGCCTT AAAGACTTTG ACCTCAGCCG AGAGACAGTC
    AAGTGGTACA AACTCACCAC GGTGCCCTAC TTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005159363.2
    CDS308..5383
    Translation

    Target ORF information:

    RefSeq Version XM_005159306.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha (pik3c2a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005159306.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCCCACA TATCCAGTGG CAATGGGTTT AAGTTTGACA GGCCAGCCTC TCCGGGGGTG 
    GTCCGGCCAA AAGGAATTGT GGGGAAGGAA GAGGCTTTAC GCATGGAGGT AGAGGCCTTC
    GATAAAATTA AGCGCGAGAA AAGGCACACA CTCCCAGTCA CAGCCTCTTC TGTTAATCCC
    CTTCCCAATC CAACAGCGCA AACATCCACC AGCCGGCCAG AGAAAGACCT TATTGTATTC
    TGCGAATCAG AAACACAGAA GAAGGAGCAA AAAGACAACT TTGAGGACAT CGATTTGGAG
    AACCTGACAA AGGAGGAGCT GGAAAAGCTG CTTCTGGATG ACAGTTTTGG TGCAAACAAA
    ATGACAAGAC CTTCCTCTTT GCTAGGTTGC AATCTCAGCG CATCATATCC TGGCGGACAT
    GCCTTCAACC CCTCTTCGTT CCACTGGACA CCCACTCCAA CGCATGCACA GACTCCCATC
    TTCCCCTCTG CTCCATTCGC CAAGCCTCCT TGCTCTTTCC AGAATGGCTT TAGTCCAGCA
    ATGTCTCCAT TTATTCCACC GACCCCCTTT CTCTCCTTTA CTCCCATCCA ACCACCTGCT
    GCTCTGGTCT ACTCACAGCC TGCCGTCACC CCAGAGATGG CCAAGCTGTT TGACAAGATT
    GCCAGCACTT CGGAATACTT GAAGAACGGC AGGTCCTCTA GCATGGAAAC CGAATCGGCA
    AGTGTCAAGT CTCTGGAGCC CCTACCTCAG CCCTCAGAAC CTCCTAACAT CAGCCGATTT
    GAATGGCTGG ACTTGGACCC TCTCAACAAG CGCAAGGAGG TGGAGGTCGA GGAGACACCT
    GCTGTTTCAA GCTGTCCTTT TATTGAGGAG TCTGGGAAAG CTAAAGACCC GTGGGATGCG
    GTTCTTCAGG ATGAGCATGA AACTGTAGAT AATGGCAGTC CCAAAGCAGA GGAAAAGTGC
    AAGGCAACTC AACCCAGAAG AGCATCCACA GGGGCGGCTG TAACAAAAAG CCAATCCCTC
    AGTATATCTT CAAACTCAAC GAATCAAAGC ACAGGCAAAC AGGTCAATAA GGGAAGCACA
    AATGTACTGT CCAAGTACTC TGCCTTACAG GAGAAGGAAG CCCAGAATCT AGAAGTTGTA
    GCATTCTGTG AAGACATTTC AAAACTGAGG TCAAAGTTCC CACACGATGA CCTGTCTACC
    AACCCTGGCT ATGTTCTCAG CCCTGTGATC ACGCAGAGGG ATGCAGGAGG GGACAATGGG
    GGCAGTGTGA AAGTGTCCAT TGAGATTTCT GATTCTCAAC AGCCTGTAAC GTTTACTTGC
    GATGTGACTT CTCCAGTGGA CTTGCTCATA ATGCAGGCTT TGTGTTGGGT TCATGATGAC
    CTGAACCAGG TGGACATCAA TAGCTACGTT CTCAAGGTCT GCGGACAGGA GGAGGTCCTT
    CAGAATAAAC ACAGTCTAGG CAGTCATGAG TACGTGCAGA ACTGCAGGAA GTGGGAATCA
    GAGATCAAAC TACAGCTCCT GTTTCTTAGC ACAATGAGAA GAGATCTGGC CCGAACTGCA
    GAAGATGACA TCTCGCCCAT TGATTTGGAG AAATACCTTG GTCTAGTGGA GAGGCCATTT
    AAAGAGGCAG TCACAAGAGA AGGCCTTGCT GAATATCTAG AAGGCTTTCA TAAGCAAGTC
    AATCTCTGCC TTCAGAATGA GAACAACCAA TATAAAACAG TGGACAATGT GGTTCAGTCT
    GTTAAGAATC TGTGCTGTGC TCTGGATGAA GTTGAGACAC CAGCCATCAC TGATGCCGTC
    AAGAGGCTCA AGCGCTCTGT CAACCTTCCT AGAACACGCT CACCAGAGCA CTATGCTGAA
    TTGCAGGCTG GTGCCACATC TTCCGGAGGT TCAGCTAATG GTTACTCTAG CCCTGTAGAG
    GAGAGTTTAG CAGTGCTCAC AGATGCTGTC TATGAACTCG CCAAGCTCTA CCTTCAGTCT
    TTCTGCCCGA TTAGCCCTGG TTTCATGATA GAAGAGCACA AAGAAGATGA GAGGGACAGC
    AAAGAGGCCT CGGGCACCAC TGAACACTTA CAGTTCACAC TGTTTGCCGT TCATGGCATT
    CCTGCTACCT GGGTCAGCAG TTTTGAAAAA TACTACCTGA TGTGTGCCCT GACCCATAAC
    AACAGGAATC TCTTCAAACC TGTCCAGTCC AAAAAAGTGG GAACATACAA GAGTTTCTTC
    TACCACATCA AATGGGATGA ATTAATAAAC TTCCCTATTT CAGTGTCGCT GTTACCACTG
    GAGGCTATGC TTAGTCTTTC TCTCTATGGG GTCCTCAGTC AGAATGCAAA CAACTCACCA
    GATTCCAACA AGCAACGGAA AGGTCCAGAA CTTTTGGGAA AAGTTTCCAT GCCTCTGTTT
    GACTTCAGGA GGGTTCTGTC CAGAGGCAGT AAACTGCTGA GTTTGTGGAC TTCTCCTCAA
    GCCCTTCAGC CTGGAGCTGC AGGCAAAGGG AGGAATCCTA CAGAAAAGAT CATACTGCAG
    GTTGACTTCC CCAGTCCAGC GGTGGACGTG CTCTATGTTG GTCCTCAGGA GAACGGTTGC
    CCCAATCCTC AGTCTCTAGA CCCACTTGAT CAAGACGATC GAAGTGAGAT AGAAAAGCTG
    TGCGCACGAG CATCTACTTT CGGACTGTCA CGGGCGGACA GACAGCTGCT TTGGGACCAG
    AGGTACTATT GTCGGGAATA TGAATACAGT TTGCCGAAAA TCCTGGCCAG CGCTCCCAGC
    TGGGACTGGG GCAGTATGGG GGAAATCCAC GCACTTCTGC ACCACTGGCC TGCTCTGTCA
    CCCGTTTCAG CTCTTGAGCT CCTTGAATCC AAGTTTGCCG ACACAGAGGT GAGGAAAGTG
    GCTGTGAGCT GGATCCAGAG CAGCAGTGAT GATGAGCTGG CTGATTATCT CCCCCAGTTA
    GTGCAGGCAG TGAAGTTCGA GTGTCACCTC AAAAATGCCT TGGTGAAGTT TCTGCTGTCT
    CGAGCTCTGG GAAATGTCAA CATTGCACAT TATCTTTACT GGCTGCTGAG GGATGCAGTG
    CAGGACCCGG CGTTTGGTCA GCGTTATGAG CGGATCCTCT GTGTGCTGCT GTGTCTGTGT
    GGGACCGGAC TAAGGGCTGA GCTGGAGAAG CAGACCCGAC TTGTGCAGCT GCTTGGAGCT
    CTGGCTGAAA AGGTTCGGCA GGCGAGCAGC TCAACCCGAC AGGTGGTTCT CCTCGAGGGT
    CTAGAGAGGG TTCAGTCTTT CTTCCAGAAG AACAGCTGTC GACTCCCTAT TAGCCCCAGT
    CTGGTGGCAA AAGAGCTCAA TATCAAGGTC TGCTCCTTTT TCAATTCCAA CGCTGTACCT
    CTAAAGATCG CACTGGTTAA CGCAGATCCG CTGGGAGATG AGATTAACGT CATGTTTAAG
    GTTGGAGAAG ACCTGCGGCA GGACATGTTG GCACTACAGA TGATTCGGAT TATGGATCGG
    ATCTGGCTTC AGGAAGGACT CGACCTGCGT ATTGTTAACT TTAAATGCAT CTCTACTGGG
    AAAGATAAAG GCATGGTGGA GTTGGTACCA TCTTCTGAAA CACTGAGAAA GATTCAGGTG
    GAGTATGGAG TGACGGGTTC CTTCAAGGAC AAACCTCTTG CTGAGTGGCT TCGCAAGTAT
    AACCCTGCTG AGGATGAGTA TGAGAAGGCG TCTGAGAATT TTATCTACAC CTGTGCTGGA
    TGCTGTGTGG CCACTTACAT CCTCGGCATC TGCGATCGTC ACAACGACAA CATCATGCTG
    CGCTCCACTG GCCACATGTT CCACATAGAC TTCGGGAAGT TCCTGGGTCA CGCACAAATG
    ATTGGGAGTT TTAAAAGGGA CCGAGCACCG TTTGTCTTAA CCTCTGACAT GGCTTATGTC
    ATCAATGGAG GCGAAAGACC CACTAGTCGA TTTCAGCTGT TCGTTGACCT TTGCTCGCAA
    GCCTACAACC TGATCCGCAA ACACTCCAAC CTCTTCCTCA ACCTGCTATC TCTGATGACA
    CAATCAGGTT TACCCGAGCT GACCGGAGTC CAAGATTTGA AGTATGTGTA TGATGCTCTT
    CAGCCTCAGA CTACAGATGC AGAGGCTACC ATTTTCTTCA CAAGGTTGAT TGAATCCAGT
    CTGGGCAGTG TGGCTACAAA GTTTAATTTC TTTATCCACA ATCTGGCTCA GTTGCGTTTC
    TCTGGCCTTC CCTCCAATGA TGAACCCATC CTGTCGTTTG CTCCTCGGAC ATACACTATG
    AAGCAAGACG GCAAGATTCG GGATGCCTCT GTGTTTTCAT ATCAAAAGAG ATATAACCCA
    GACAAGCACT ATACGTATGT CATTCGGATT CTAAGGGAAG GTCAGAGCGA GCCACAGTTT
    GTTTTCCGCA CATTTGATGA GTTTCAGGAA TTGCACAACA AATTGACCAT CCTTTTTCAG
    TTGTGGAAGC TGCCAGGGTT TCCCAGTAAG ATGGTGCTGG GACGCACACA CATCAAGGAT
    GTGGCATCCA AGAGAAAGGT TGAATTGAAT AGTTATGTGC ACAATCTGAT GAGGAGTTCA
    ACAGAGGTCG CCCAGTGTGA TCTGATCTAC ACCTTCTTCC ACCCTATCGC AAGAGATGAC
    AAGACTGAAG GGGTTGATAC CCTATCAAAA ACTCCAGACA TGCCTCCAGT GAGTCCCACC
    ACAGGCCGTG TGGAGGGTGA GGTGAAGCTG TCAGTGTCCT ACAGAAACAG CACACTTTTC
    ATCATGGTCA TGCACATTAA AGACCTGATG TCCAATGACG GAGCAGACCC AAACCCTTAT
    GTGAAAACAT ACCTTCTCCC AGACCCCCAC AAAACTTCCA AACGCAAAAC AAAGATTGCG
    AGGAAGACAA GGAACCCAAC ATTTAATGAA ATGCTGGTGT ATAGCGGTTA CAGTAAGGAA
    ACACTGAAGC AAAGGGAACT TCAACTCAGT GTGCTCAGTG CCGAGTCTCT GCGAGAGAAC
    TGCTATTTGG GAGGAATCAC CCTCTGCCTT AAAGACTTTG ACCTCAGCCG AGAGACAGTC
    AAGTGGTACA AACTCACCAC GGTGCCCTAC TTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa146023
    Clone ID Related Accession (Same CDS sequence) XM_694937.8
    Accession Version XM_694937.8 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395030.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_694937.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCCCACA TATCCAGTGG CAATGGGTTT AAGTTTGACA GGCCAGCCTC TCCGGGGGTG 
    GTCCGGCCAA AAGGAATTGT GGGGAAGGAA GAGGCTTTAC GCATGGAGGT AGAGGCCTTC
    GATAAAATTA AGCGCGAGAA AAGGCACACA CTCCCAGTCA CAGCCTCTTC TGTTAATCCC
    CTTCCCAATC CAACAGCGCA AACATCCACC AGCCGGCCAG AGAAAGACCT TATTGTATTC
    TGCGAATCAG AAACACAGAA GAAGGAGCAA AAAGACAACT TTGAGGACAT CGATTTGGAG
    AACCTGACAA AGGAGGAGCT GGAAAAGCTG CTTCTGGATG ACAGTTTTGG TGCAAACAAA
    ATGACAAGAC CTTCCTCTTT GCTAGGTTGC AATCTCAGCG CATCATATCC TGGCGGACAT
    GCCTTCAACC CCTCTTCGTT CCACTGGACA CCCACTCCAA CGCATGCACA GACTCCCATC
    TTCCCCTCTG CTCCATTCGC CAAGCCTCCT TGCTCTTTCC AGAATGGCTT TAGTCCAGCA
    ATGTCTCCAT TTATTCCACC GACCCCCTTT CTCTCCTTTA CTCCCATCCA ACCACCTGCT
    GCTCTGGTCT ACTCACAGCC TGCCGTCACC CCAGAGATGG CCAAGCTGTT TGACAAGATT
    GCCAGCACTT CGGAATACTT GAAGAACGGC AGGTCCTCTA GCATGGAAAC CGAATCGGCA
    AGTGTCAAGT CTCTGGAGCC CCTACCTCAG CCCTCAGAAC CTCCTAACAT CAGCCGATTT
    GAATGGCTGG ACTTGGACCC TCTCAACAAG CGCAAGGAGG TGGAGGTCGA GGAGACACCT
    GCTGTTTCAA GCTGTCCTTT TATTGAGGAG TCTGGGAAAG CTAAAGACCC GTGGGATGCG
    GTTCTTCAGG ATGAGCATGA AACTGTAGAT AATGGCAGTC CCAAAGCAGA GGAAAAGTGC
    AAGGCAACTC AACCCAGAAG AGCATCCACA GGGGCGGCTG TAACAAAAAG CCAATCCCTC
    AGTATATCTT CAAACTCAAC GAATCAAAGC ACAGGCAAAC AGGTCAATAA GGGAAGCACA
    AATGTACTGT CCAAGTACTC TGCCTTACAG GAGAAGGAAG CCCAGAATCT AGAAGTTGTA
    GCATTCTGTG AAGACATTTC AAAACTGAGG TCAAAGTTCC CACACGATGA CCTGTCTACC
    AACCCTGGCT ATGTTCTCAG CCCTGTGATC ACGCAGAGGG ATGCAGGAGG GGACAATGGG
    GGCAGTGTGA AAGTGTCCAT TGAGATTTCT GATTCTCAAC AGCCTGTAAC GTTTACTTGC
    GATGTGACTT CTCCAGTGGA CTTGCTCATA ATGCAGGCTT TGTGTTGGGT TCATGATGAC
    CTGAACCAGG TGGACATCAA TAGCTACGTT CTCAAGGTCT GCGGACAGGA GGAGGTCCTT
    CAGAATAAAC ACAGTCTAGG CAGTCATGAG TACGTGCAGA ACTGCAGGAA GTGGGAATCA
    GAGATCAAAC TACAGCTCCT GTTTCTTAGC ACAATGAGAA GAGATCTGGC CCGAACTGCA
    GAAGATGACA TCTCGCCCAT TGATTTGGAG AAATACCTTG GTCTAGTGGA GAGGCCATTT
    AAAGAGGCAG TCACAAGAGA AGGCCTTGCT GAATATCTAG AAGGCTTTCA TAAGCAAGTC
    AATCTCTGCC TTCAGAATGA GAACAACCAA TATAAAACAG TGGACAATGT GGTTCAGTCT
    GTTAAGAATC TGTGCTGTGC TCTGGATGAA GTTGAGACAC CAGCCATCAC TGATGCCGTC
    AAGAGGCTCA AGCGCTCTGT CAACCTTCCT AGAACACGCT CACCAGAGGC TGGTGCCACA
    TCTTCCGGAG GTTCAGCTAA TGGTTACTCT AGCCCTGTAG AGGAGAGTTT AGCAGTGCTC
    ACAGATGCTG TCTATGAACT CGCCAAGCTC TACCTTCAGT CTTTCTGCCC GATTAGCCCT
    GGTTTCATGA TAGAAGAGCA CAAAGAAGAT GAGAGGGACA GCAAAGAGGC CTCGGGCACC
    ACTGAACACT TACAGTTCAC ACTGTTTGCC GTTCATGGCA TTCCTGCTAC CTGGGTCAGC
    AGTTTTGAAA AATACTACCT GATGTGTGCC CTGACCCATA ACAACAGGAA TCTCTTCAAA
    CCTGTCCAGT CCAAAAAAGT GGGAACATAC AAGAGTTTCT TCTACCACAT CAAATGGGAT
    GAATTAATAA ACTTCCCTAT TTCAGTGTCG CTGTTACCAC TGGAGGCTAT GCTTAGTCTT
    TCTCTCTATG GGGTCCTCAG TCAGAATGCA AACAACTCAC CAGATTCCAA CAAGCAACGG
    AAAGGTCCAG AACTTTTGGG AAAAGTTTCC ATGCCTCTGT TTGACTTCAG GAGGGTTCTG
    TCCAGAGGCA GTAAACTGCT GAGTTTGTGG ACTTCTCCTC AAGCCCTTCA GCCTGGAGCT
    GCAGGCAAAG GGAGGAATCC TACAGAAAAG ATCATACTGC AGGTTGACTT CCCCAGTCCA
    GCGGTGGACG TGCTCTATGT TGGTCCTCAG GAGAACGGTT GCCCCAATCC TCAGTCTCTA
    GACCCACTTG ATCAAGACGA TCGAAGTGAG ATAGAAAAGC TGTGCGCACG AGCATCTACT
    TTCGGACTGT CACGGGCGGA CAGACAGCTG CTTTGGGACC AGAGGTACTA TTGTCGGGAA
    TATGAATACA GTTTGCCGAA AATCCTGGCC AGCGCTCCCA GCTGGGACTG GGGCAGTATG
    GGGGAAATCC ACGCACTTCT GCACCACTGG CCTGCTCTGT CACCCGTTTC AGCTCTTGAG
    CTCCTTGAAT CCAAGTTTGC CGACACAGAG GTGAGGAAAG TGGCTGTGAG CTGGATCCAG
    AGCAGCAGTG ATGATGAGCT GGCTGATTAT CTCCCCCAGT TAGTGCAGGC AGTGAAGTTC
    GAGTGTCACC TCAAAAATGC CTTGGTGAAG TTTCTGCTGT CTCGAGCTCT GGGAAATGTC
    AACATTGCAC ATTATCTTTA CTGGCTGCTG AGGGATGCAG TGCAGGACCC GGCGTTTGGT
    CAGCGTTATG AGCGGATCCT CTGTGTGCTG CTGTGTCTGT GTGGGACCGG ACTAAGGGCT
    GAGCTGGAGA AGCAGACCCG ACTTGTGCAG CTGCTTGGAG CTCTGGCTGA AAAGGTTCGG
    CAGGCGAGCA GCTCAACCCG ACAGGTGGTT CTCCTCGAGG GTCTAGAGAG GGTTCAGTCT
    TTCTTCCAGA AGAACAGCTG TCGACTCCCT ATTAGCCCCA GTCTGGTGGC AAAAGAGCTC
    AATATCAAGG TCTGCTCCTT TTTCAATTCC AACGCTGTAC CTCTAAAGAT CGCACTGGTT
    AACGCAGATC CGCTGGGAGA TGAGATTAAC GTCATGTTTA AGGTTGGAGA AGACCTGCGG
    CAGGACATGT TGGCACTACA GATGATTCGG ATTATGGATC GGATCTGGCT TCAGGAAGGA
    CTCGACCTGC GTATTGTTAA CTTTAAATGC ATCTCTACTG GGAAAGATAA AGGCATGGTG
    GAGTTGGTAC CATCTTCTGA AACACTGAGA AAGATTCAGG TGGAGTATGG AGTGACGGGT
    TCCTTCAAGG ACAAACCTCT TGCTGAGTGG CTTCGCAAGT ATAACCCTGC TGAGGATGAG
    TATGAGAAGG CGTCTGAGAA TTTTATCTAC ACCTGTGCTG GATGCTGTGT GGCCACTTAC
    ATCCTCGGCA TCTGCGATCG TCACAACGAC AACATCATGC TGCGCTCCAC TGGCCACATG
    TTCCACATAG ACTTCGGGAA GTTCCTGGGT CACGCACAAA TGATTGGGAG TTTTAAAAGG
    GACCGAGCAC CGTTTGTCTT AACCTCTGAC ATGGCTTATG TCATCAATGG AGGCGAAAGA
    CCCACTAGTC GATTTCAGCT GTTCGTTGAC CTTTGCTCGC AAGCCTACAA CCTGATCCGC
    AAACACTCCA ACCTCTTCCT CAACCTGCTA TCTCTGATGA CACAATCAGG TTTACCCGAG
    CTGACCGGAG TCCAAGATTT GAAGTATGTG TATGATGCTC TTCAGCCTCA GACTACAGAT
    GCAGAGGCTA CCATTTTCTT CACAAGGTTG ATTGAATCCA GTCTGGGCAG TGTGGCTACA
    AAGTTTAATT TCTTTATCCA CAATCTGGCT CAGTTGCGTT TCTCTGGCCT TCCCTCCAAT
    GATGAACCCA TCCTGTCGTT TGCTCCTCGG ACATACACTA TGAAGCAAGA CGGCAAGATT
    CGGGATGCCT CTGTGTTTTC ATATCAAAAG AGATATAACC CAGACAAGCA CTATACGTAT
    GTCATTCGGA TTCTAAGGGA AGGTCAGAGC GAGCCACAGT TTGTTTTCCG CACATTTGAT
    GAGTTTCAGG AATTGCACAA CAAATTGACC ATCCTTTTTC AGTTGTGGAA GCTGCCAGGG
    TTTCCCAGTA AGATGGTGCT GGGACGCACA CACATCAAGG ATGTGGCATC CAAGAGAAAG
    GTTGAATTGA ATAGTTATGT GCACAATCTG ATGAGGAGTT CAACAGAGGT CGCCCAGTGT
    GATCTGATCT ACACCTTCTT CCACCCTATC GCAAGAGATG ACAAGACTGA AGGGGTTGAT
    ACCCTATCAA AAACTCCAGA CATGCCTCCA GTGAGTCCCA CCACAGGCCG TGTGGAGGGT
    GAGGTGAAGC TGTCAGTGTC CTACAGAAAC AGCACACTTT TCATCATGGT CATGCACATT
    AAAGACCTGA TGTCCAATGA CGGAGCAGAC CCAAACCCTT ATGTGAAAAC ATACCTTCTC
    CCAGACCCCC ACAAAACTTC CAAACGCAAA ACAAAGATTG CGAGGAAGAC AAGGAACCCA
    ACATTTAATG AAATGCTGGT GTATAGCGGT TACAGTAAGG AAACACTGAA GCAAAGGGAA
    CTTCAACTCA GTGTGCTCAG TGCCGAGTCT CTGCGAGAGA ACTGCTATTT GGGAGGAATC
    ACCCTCTGCC TTAAAGACTT TGACCTCAGC CGAGAGACAG TCAAGTGGTA CAAACTCACC
    ACGGTGCCCT ACTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_700029.5
    CDS177..5234
    Translation

    Target ORF information:

    RefSeq Version XM_694937.8
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha (pik3c2a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_694937.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCCCACA TATCCAGTGG CAATGGGTTT AAGTTTGACA GGCCAGCCTC TCCGGGGGTG 
    GTCCGGCCAA AAGGAATTGT GGGGAAGGAA GAGGCTTTAC GCATGGAGGT AGAGGCCTTC
    GATAAAATTA AGCGCGAGAA AAGGCACACA CTCCCAGTCA CAGCCTCTTC TGTTAATCCC
    CTTCCCAATC CAACAGCGCA AACATCCACC AGCCGGCCAG AGAAAGACCT TATTGTATTC
    TGCGAATCAG AAACACAGAA GAAGGAGCAA AAAGACAACT TTGAGGACAT CGATTTGGAG
    AACCTGACAA AGGAGGAGCT GGAAAAGCTG CTTCTGGATG ACAGTTTTGG TGCAAACAAA
    ATGACAAGAC CTTCCTCTTT GCTAGGTTGC AATCTCAGCG CATCATATCC TGGCGGACAT
    GCCTTCAACC CCTCTTCGTT CCACTGGACA CCCACTCCAA CGCATGCACA GACTCCCATC
    TTCCCCTCTG CTCCATTCGC CAAGCCTCCT TGCTCTTTCC AGAATGGCTT TAGTCCAGCA
    ATGTCTCCAT TTATTCCACC GACCCCCTTT CTCTCCTTTA CTCCCATCCA ACCACCTGCT
    GCTCTGGTCT ACTCACAGCC TGCCGTCACC CCAGAGATGG CCAAGCTGTT TGACAAGATT
    GCCAGCACTT CGGAATACTT GAAGAACGGC AGGTCCTCTA GCATGGAAAC CGAATCGGCA
    AGTGTCAAGT CTCTGGAGCC CCTACCTCAG CCCTCAGAAC CTCCTAACAT CAGCCGATTT
    GAATGGCTGG ACTTGGACCC TCTCAACAAG CGCAAGGAGG TGGAGGTCGA GGAGACACCT
    GCTGTTTCAA GCTGTCCTTT TATTGAGGAG TCTGGGAAAG CTAAAGACCC GTGGGATGCG
    GTTCTTCAGG ATGAGCATGA AACTGTAGAT AATGGCAGTC CCAAAGCAGA GGAAAAGTGC
    AAGGCAACTC AACCCAGAAG AGCATCCACA GGGGCGGCTG TAACAAAAAG CCAATCCCTC
    AGTATATCTT CAAACTCAAC GAATCAAAGC ACAGGCAAAC AGGTCAATAA GGGAAGCACA
    AATGTACTGT CCAAGTACTC TGCCTTACAG GAGAAGGAAG CCCAGAATCT AGAAGTTGTA
    GCATTCTGTG AAGACATTTC AAAACTGAGG TCAAAGTTCC CACACGATGA CCTGTCTACC
    AACCCTGGCT ATGTTCTCAG CCCTGTGATC ACGCAGAGGG ATGCAGGAGG GGACAATGGG
    GGCAGTGTGA AAGTGTCCAT TGAGATTTCT GATTCTCAAC AGCCTGTAAC GTTTACTTGC
    GATGTGACTT CTCCAGTGGA CTTGCTCATA ATGCAGGCTT TGTGTTGGGT TCATGATGAC
    CTGAACCAGG TGGACATCAA TAGCTACGTT CTCAAGGTCT GCGGACAGGA GGAGGTCCTT
    CAGAATAAAC ACAGTCTAGG CAGTCATGAG TACGTGCAGA ACTGCAGGAA GTGGGAATCA
    GAGATCAAAC TACAGCTCCT GTTTCTTAGC ACAATGAGAA GAGATCTGGC CCGAACTGCA
    GAAGATGACA TCTCGCCCAT TGATTTGGAG AAATACCTTG GTCTAGTGGA GAGGCCATTT
    AAAGAGGCAG TCACAAGAGA AGGCCTTGCT GAATATCTAG AAGGCTTTCA TAAGCAAGTC
    AATCTCTGCC TTCAGAATGA GAACAACCAA TATAAAACAG TGGACAATGT GGTTCAGTCT
    GTTAAGAATC TGTGCTGTGC TCTGGATGAA GTTGAGACAC CAGCCATCAC TGATGCCGTC
    AAGAGGCTCA AGCGCTCTGT CAACCTTCCT AGAACACGCT CACCAGAGGC TGGTGCCACA
    TCTTCCGGAG GTTCAGCTAA TGGTTACTCT AGCCCTGTAG AGGAGAGTTT AGCAGTGCTC
    ACAGATGCTG TCTATGAACT CGCCAAGCTC TACCTTCAGT CTTTCTGCCC GATTAGCCCT
    GGTTTCATGA TAGAAGAGCA CAAAGAAGAT GAGAGGGACA GCAAAGAGGC CTCGGGCACC
    ACTGAACACT TACAGTTCAC ACTGTTTGCC GTTCATGGCA TTCCTGCTAC CTGGGTCAGC
    AGTTTTGAAA AATACTACCT GATGTGTGCC CTGACCCATA ACAACAGGAA TCTCTTCAAA
    CCTGTCCAGT CCAAAAAAGT GGGAACATAC AAGAGTTTCT TCTACCACAT CAAATGGGAT
    GAATTAATAA ACTTCCCTAT TTCAGTGTCG CTGTTACCAC TGGAGGCTAT GCTTAGTCTT
    TCTCTCTATG GGGTCCTCAG TCAGAATGCA AACAACTCAC CAGATTCCAA CAAGCAACGG
    AAAGGTCCAG AACTTTTGGG AAAAGTTTCC ATGCCTCTGT TTGACTTCAG GAGGGTTCTG
    TCCAGAGGCA GTAAACTGCT GAGTTTGTGG ACTTCTCCTC AAGCCCTTCA GCCTGGAGCT
    GCAGGCAAAG GGAGGAATCC TACAGAAAAG ATCATACTGC AGGTTGACTT CCCCAGTCCA
    GCGGTGGACG TGCTCTATGT TGGTCCTCAG GAGAACGGTT GCCCCAATCC TCAGTCTCTA
    GACCCACTTG ATCAAGACGA TCGAAGTGAG ATAGAAAAGC TGTGCGCACG AGCATCTACT
    TTCGGACTGT CACGGGCGGA CAGACAGCTG CTTTGGGACC AGAGGTACTA TTGTCGGGAA
    TATGAATACA GTTTGCCGAA AATCCTGGCC AGCGCTCCCA GCTGGGACTG GGGCAGTATG
    GGGGAAATCC ACGCACTTCT GCACCACTGG CCTGCTCTGT CACCCGTTTC AGCTCTTGAG
    CTCCTTGAAT CCAAGTTTGC CGACACAGAG GTGAGGAAAG TGGCTGTGAG CTGGATCCAG
    AGCAGCAGTG ATGATGAGCT GGCTGATTAT CTCCCCCAGT TAGTGCAGGC AGTGAAGTTC
    GAGTGTCACC TCAAAAATGC CTTGGTGAAG TTTCTGCTGT CTCGAGCTCT GGGAAATGTC
    AACATTGCAC ATTATCTTTA CTGGCTGCTG AGGGATGCAG TGCAGGACCC GGCGTTTGGT
    CAGCGTTATG AGCGGATCCT CTGTGTGCTG CTGTGTCTGT GTGGGACCGG ACTAAGGGCT
    GAGCTGGAGA AGCAGACCCG ACTTGTGCAG CTGCTTGGAG CTCTGGCTGA AAAGGTTCGG
    CAGGCGAGCA GCTCAACCCG ACAGGTGGTT CTCCTCGAGG GTCTAGAGAG GGTTCAGTCT
    TTCTTCCAGA AGAACAGCTG TCGACTCCCT ATTAGCCCCA GTCTGGTGGC AAAAGAGCTC
    AATATCAAGG TCTGCTCCTT TTTCAATTCC AACGCTGTAC CTCTAAAGAT CGCACTGGTT
    AACGCAGATC CGCTGGGAGA TGAGATTAAC GTCATGTTTA AGGTTGGAGA AGACCTGCGG
    CAGGACATGT TGGCACTACA GATGATTCGG ATTATGGATC GGATCTGGCT TCAGGAAGGA
    CTCGACCTGC GTATTGTTAA CTTTAAATGC ATCTCTACTG GGAAAGATAA AGGCATGGTG
    GAGTTGGTAC CATCTTCTGA AACACTGAGA AAGATTCAGG TGGAGTATGG AGTGACGGGT
    TCCTTCAAGG ACAAACCTCT TGCTGAGTGG CTTCGCAAGT ATAACCCTGC TGAGGATGAG
    TATGAGAAGG CGTCTGAGAA TTTTATCTAC ACCTGTGCTG GATGCTGTGT GGCCACTTAC
    ATCCTCGGCA TCTGCGATCG TCACAACGAC AACATCATGC TGCGCTCCAC TGGCCACATG
    TTCCACATAG ACTTCGGGAA GTTCCTGGGT CACGCACAAA TGATTGGGAG TTTTAAAAGG
    GACCGAGCAC CGTTTGTCTT AACCTCTGAC ATGGCTTATG TCATCAATGG AGGCGAAAGA
    CCCACTAGTC GATTTCAGCT GTTCGTTGAC CTTTGCTCGC AAGCCTACAA CCTGATCCGC
    AAACACTCCA ACCTCTTCCT CAACCTGCTA TCTCTGATGA CACAATCAGG TTTACCCGAG
    CTGACCGGAG TCCAAGATTT GAAGTATGTG TATGATGCTC TTCAGCCTCA GACTACAGAT
    GCAGAGGCTA CCATTTTCTT CACAAGGTTG ATTGAATCCA GTCTGGGCAG TGTGGCTACA
    AAGTTTAATT TCTTTATCCA CAATCTGGCT CAGTTGCGTT TCTCTGGCCT TCCCTCCAAT
    GATGAACCCA TCCTGTCGTT TGCTCCTCGG ACATACACTA TGAAGCAAGA CGGCAAGATT
    CGGGATGCCT CTGTGTTTTC ATATCAAAAG AGATATAACC CAGACAAGCA CTATACGTAT
    GTCATTCGGA TTCTAAGGGA AGGTCAGAGC GAGCCACAGT TTGTTTTCCG CACATTTGAT
    GAGTTTCAGG AATTGCACAA CAAATTGACC ATCCTTTTTC AGTTGTGGAA GCTGCCAGGG
    TTTCCCAGTA AGATGGTGCT GGGACGCACA CACATCAAGG ATGTGGCATC CAAGAGAAAG
    GTTGAATTGA ATAGTTATGT GCACAATCTG ATGAGGAGTT CAACAGAGGT CGCCCAGTGT
    GATCTGATCT ACACCTTCTT CCACCCTATC GCAAGAGATG ACAAGACTGA AGGGGTTGAT
    ACCCTATCAA AAACTCCAGA CATGCCTCCA GTGAGTCCCA CCACAGGCCG TGTGGAGGGT
    GAGGTGAAGC TGTCAGTGTC CTACAGAAAC AGCACACTTT TCATCATGGT CATGCACATT
    AAAGACCTGA TGTCCAATGA CGGAGCAGAC CCAAACCCTT ATGTGAAAAC ATACCTTCTC
    CCAGACCCCC ACAAAACTTC CAAACGCAAA ACAAAGATTG CGAGGAAGAC AAGGAACCCA
    ACATTTAATG AAATGCTGGT GTATAGCGGT TACAGTAAGG AAACACTGAA GCAAAGGGAA
    CTTCAACTCA GTGTGCTCAG TGCCGAGTCT CTGCGAGAGA ACTGCTATTT GGGAGGAATC
    ACCCTCTGCC TTAAAGACTT TGACCTCAGC CGAGAGACAG TCAAGTGGTA CAAACTCACC
    ACGGTGCCCT ACTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23385
    Clone ID Related Accession (Same CDS sequence) XM_021467820.1 , XM_005159305.3 , XM_005159306.2 , XM_021467821.1
    Accession Version XM_021467820.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCCCACA TATCCAGTGG CAATGGGTTT AAGTTTGACA GGCCAGCCTC TCCGGGGGTG 
    GTCCGGCCAA AAGGAATTGT GGGGAAGGAA GAGGCTTTAC GCATGGAGGT AGAGGCCTTC
    GATAAAATTA AGCGCGAGAA AAGGCACACA CTCCCAGTCA CAGCCTCTTC TGTTAATCCC
    CTTCCCAATC CAACAGCGCA AACATCCAGC AGCCGGCCAG AGAAAGACCT TATTGTATTC
    TGCGAATCAG AAACACAGAA GAAGGAGCAA AAAGACAACT TTGAGGACAT CGATTTGGAG
    AACCTGACAA AGGAGGAGCT GGAAAAGCTG CTTCTGGATG ACAGTTTTGG TGCAAACAAA
    ATGACAAGAC CTTCCTCTTT GCTAGGTTGC AATCTCAGCG CATCATATCC TGGCGGACAT
    GCCTTCAACC CCTCTTCGTT CCACTGGACA CCCACTCCAA CGCATGCACA GACTCCCATC
    TTCCCCTCTG CTCCATTCGC CAAGCCTCCT TGCTCTTTCC AGAATGGCTT TAGTCCAGCA
    ATGTCTCCAT TTATTCCACC GACCCCCTTT CTCTCCTTTA CTCCCATCCA ACCACCTGCT
    GCTCTGGTCT ACTCGCAGCC TGCCGTCACC CCAGAGATGG CCAAGCTGTT TGACAAGATT
    GCCAGCACTT CGGAATACTT GAAGAACGGC AGGTCCTCTA GCATGGAAAC CGAATCGGCA
    AGTGTCAAGT CTCTGGAGCC CCTACCTCTG CCCTCAGAAC CTCCTAACAT CAGCCGATTT
    GAATGGCTGG ACTTGGACCC TCTCAACAAG CGCAAGGAGG TGGAGGTCGA GGAGACACCT
    GCTGTTTCAA GCTGTCCTTT TATTGAGGAG TCTGGGAAAG CTAAAGACCC GTGGGATGCG
    GTTCTTCAGG ATGAGCATGA AACTGTAGAT AATGGCAGTC CCACCGCAGA GGAAAAGAGC
    AAGGCAACTC AACCCAGAAG AGCATCCACA GGGGCGGCTG TAACAAAAAG CCAATCCCTC
    AGTATATCTG CAAACTCAAC GAATCAAAGC ACAGGCAAAC AGGTCAATAA GGGAAGCACA
    AATGTACTGT CCAAGTACTC TGCCTTACAG GAGAAGGAAG CCCAGAATCT AGAAGTTGTA
    GCATTCTGTG AAGACATTTC AAAACTGAGG TCAAAGTTCC CACACGATGA CCTGTCTACC
    AACCCTGGCT ATGTTCTCAG CCCTGTGATC ACGCAGAGGG ATGCAGGAGG GGACAATGGG
    GGCAGTGTGA AAGTGTCCAT TGAGATTTCT GATTCTCAAC AGCCTGTAAC GTTTACTTGC
    GATGTGACTT CTCCAGTGGA CTTGCTCATA ATGCAGGCTT TGTGTTGGGT TCATGATGAC
    CTGAACCAGG TGGACATCAA TAGCTACGTT CTCAAGGTCT GCGGACAGGA GGAGGTCCTT
    CAGAATAAAC ACAGTCTAGG CAGTCATGAG TACGTGCAGA ACTGCAGGAA GTGGGAATCA
    GAGATCAAAC TACAGCTCCT GTTTCTTAGC ACAATGAGAA GAGATCTGGC CCGAACTGCA
    GAAGATGACA TCTCGCCCAT TGATTTGGAG AAATACCTTG GTCTAGTGGA GAGGCCATTT
    AAAGAGGCAG TCACAAGAGA AGGCCTTGCT GAATATCTAG AAGGCTTTCA TAAGCAAGTC
    AATCTCTGCC TTCAGAATGA GAACAACCAA TATAAAACAG TGGACAATGT GGTTCAGTCT
    GTTAAGAATC TGTGCTGTGC TCTGGATGAA GTTGAGACAC CAGCCATCAC TGATGCCGTC
    AAGAGGCTCA AGCGCTCTGT CAACCTTCCT AGAACACGCT CACCAGAGCA CTATGCTGAA
    TTGCAGGCTG GTGCCACATC TTCCGGAGGT TCAGCTAATG GTTACTCTAG CCCTGTAGAG
    GAGAGTTTAG CAGTGCTCAC AGATGCTGTC TATGAACTCG CCAAGCTCTA CCTTCAGTCT
    TTCTGCCCGA TTAGCCCTGG TTTCATGATA GAAGAGCACA AAGAAGATGA GAGGGACAGC
    AAAGAGGCCT CGGGCACCAC TGAACACTTA CAGTTCACAC TGTTTGCCGT TCATGGCATT
    CCTGCTACCT GGGTCAGCAG TTTTGAAAAA TACTACCTGA TGTGTGCCCT GACCCATAAC
    AACAGGAATC TCTTCAAACC TGTCCAGTCC AAAAAAGTGG GAACATACAA GAGTTTCTTC
    TACCACATCA AATGGGATGA ATTAATAAAC TTCCCTATTT CAGTGTCGCT GTTACCACTG
    GAGGCTATGC TTAGTCTTTC TCTCTATGGG GTCCTCAGTC AGAATGCAAA CAACTCACCA
    GATTCCAACA AGCAACGGAA AGGTCCAGAA CTTTTGGGAA AAGTTTCCAT GCCTCTGTTT
    GACTTCAGGA GGGTTCTGTC CAGAGGCAGT AAACTGCTGA GTTTGTGGAC TTCTCCTCAA
    GCCCTTCAGC CTGGAGCTGC AGGCAAAGGG AGGAATCCTA CAGAAAAGAT CATACTGCAG
    GTTGACTTCC CCAGTCCAGC GGTGGACGTG CTCTATGTTG GTCCTCAGGA GAACGGCTGC
    CCCAATCCTC AGTCTCTAGA CCCACTTGAT CAAGACGATC GAAGTGAGAT AGAAAAGCTG
    TGCGCACGAG CATCTACTTT CGGACTGTCA CGGGCGGACA GACAGCTGCT TTGGGACCAG
    AGGTACTATT GTCGGGAATA TGAATACAGT TTGCCGAAAA TCCTGGCCAG CGCTCCCAGC
    TGGGACTGGG GCAGTATGGG GGAAATCCAC GCACTTCTGC ACCACTGGCC TGCTCTGTCA
    CCCGTTTCAG CTCTTGAGCT CCTTGAATCC AAGTTTGCCG ACACAGAGGT GAGGAAAGTG
    GCTGTGAGCT GGATCCAGAG CAGCAGTGAT GATGAGCTGG CTGATTATCT CCCCCAGCTA
    GTGCAGGCAG TGAAGTTCGA GTGTCACCTT AACAATGCCT TGGTGAAGTT TCTGCTGTCT
    CGAGCTCTGG GAAATGTCAA CATTGCACAT TATCTTTACT GGCTGCTGAG GGATGCAGTG
    CAGGACCCGG CGTTTGGTCA GCGTTATGAG CGGATCCTCT GTGTGCTGCT GTGTCTGTGT
    GGGACCGGAC TGAGGGCTGA GCTGGAGAAG CAGACCCGAC TTGTGCAGCT GCTTGGAGCT
    CTGGCTGAAA AGGTTCGGCA GGCGAGCAGC TCAACCCGAC AGGTGGTTCT CCTCGAGGGT
    CTAGAGAGGG TTCAGTCTTT CTTCCAGAAG AACAGCTGTC GACTCCCTAT TAGCCCCAGT
    CTGGTGGCAA AAGAGCTCAA TATCAAGGTC TGCTCCTTTT TCAATTCCAA CGCTGTACCT
    CTAAAGATCG CACTGGTTAA CGCAGATCCG CTGGGAGATG AGATTAACGT CATGTTTAAG
    GTTGGAGAAG ACCTGCGGCA GGACATGTTG GCACTACAGA TGATTCGGAT TATGGATCGG
    ATCTGGCTTC AGGAAGGACT CGACCTGCGT ATTGTTAACT TTAAATGCAT CTCTACTGGG
    AAAGATAAAG GCATGGTGGA GTTGGTACCA TCTTCTGAAA CGCTGAGAAA GATTCAGGTG
    GAGTATGGAG TGACGGGTTC CTTCAAGGAC AAACCTCTTG CTGAGTGGCT TCGCAAGTAT
    AACCCTGCTG AGGATGAGTA TGAGAAGGCG TCTGAGAATT TTATCTACAC CTGTGCTGGA
    TGCTGTGTGG CCACTTACAT CCTCGGCATC TGCGATCGTC ACAACGACAA CATCATGCTG
    CGCTCCACTG GCCACATGTT CCACATAGAC TTCGGGAAGT TCCTGGGTCA CGCACAAATG
    ATTGGGAGTT TTAAAAGGGA CCGAGCACCG TTTGTCTTAA CCTCTGACAT GGCTTATGTC
    ATCAATGGAG GCGAAAGACC CACTAGTCGC TTTCAGCTGT TCGTTGACCT TTGCTCGCAA
    GCCTACAACC TGATCCGCAA ACACTCCAAC CTCTTCCTCA ACCTGCTATC TCTGATGACA
    CAATCAGGTT TACCCGAGCT GACCGGAGTC CAAGATTTGA AGTATGTGTA TGATGCTCTT
    CAGCCTCAGA CTACAGATGC AGAGGCTACC ATTTTCTTCA CAAGGTTGAT TGAATCCAGT
    CTGGGCAGTG TGGCTACAAA GTTTAATTTC TTTATCCACA ATCTGGCTCA GTTGCGTTTC
    TCTGGCCTTC CCTCCAATGA TGAACCCATC CTGTCGTTTG CTCCTCGGAC ATACACTATG
    AAGCAAGACG GCAAGATTCG GGACGCCTCT GTGTTTTCAT TTCAAAAGAG ATATAACCCA
    GACAAGCACT ATACGTATGT CATTCGGATT CTAAGGGAAG GTCAGAGCGA GCCACAGTTT
    GTTTTCCGCA CATTTGATGA GTTTCAGGAA TTGCACAACA AATTGACCAT CCTTTTTCAG
    TTGTGGAAGC TGCCAGGGTT TCCCAGTAAG ATGGTGCTGG GACGCACACA CATCAAGGAT
    GTGGCATCCA AGAGAAAGGT TGAATTGAAT AGTTATGTGC ACAATCTGAT GAGGAGTTCA
    ACAGAGGTCG CCCAGTGTGA TCTGATCTAC ACCTTCTTCC ACCCTATCGC AAGAGATGAC
    AAGACTGAAG GGGTTGATAC CCTATCAAAA ACTCCAGACA TGCCTCCAGT GAGTCCCACC
    ACAGGCCGTG TGGAGGGTGA GGTGAAGCTG TCAGTGTCCT ACAGAAACAG CACACTTTTC
    ATCATGGTCA TGCACATTAA AGACCTGATG TCCAATGACG GAGCAGACCC AAACCCTTAT
    GTGAAAACAT ACCTTCTCCC AGACCCCCAC AAAACTTCCA AACGCAAAAC AAAGATTGCG
    AGGAAGACAA GGAACCCAAC ATTTAATGAA ATGCTGGTGT ATAGCGGTTA CAGTAAGGAA
    ACACTGAAGC AAAGGGAACT TCAACTCAGT GTGCTCAGTG CCGAGTCTCT GCGAGAGAAC
    TGCTATTTGG GAGGAATAAC CCTCTGCCTT AAAGACTTTG ACCTCAGCCG AGAGACAGTC
    AAGTGGTACA AACTCACCAC GGTGCCCTAC TTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323495.1
    CDS177..5252
    Translation

    Target ORF information:

    RefSeq Version XM_021467820.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha (pik3c2a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467820.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCCCACA TATCCAGTGG CAATGGGTTT AAGTTTGACA GGCCAGCCTC TCCGGGGGTG 
    GTCCGGCCAA AAGGAATTGT GGGGAAGGAA GAGGCTTTAC GCATGGAGGT AGAGGCCTTC
    GATAAAATTA AGCGCGAGAA AAGGCACACA CTCCCAGTCA CAGCCTCTTC TGTTAATCCC
    CTTCCCAATC CAACAGCGCA AACATCCAGC AGCCGGCCAG AGAAAGACCT TATTGTATTC
    TGCGAATCAG AAACACAGAA GAAGGAGCAA AAAGACAACT TTGAGGACAT CGATTTGGAG
    AACCTGACAA AGGAGGAGCT GGAAAAGCTG CTTCTGGATG ACAGTTTTGG TGCAAACAAA
    ATGACAAGAC CTTCCTCTTT GCTAGGTTGC AATCTCAGCG CATCATATCC TGGCGGACAT
    GCCTTCAACC CCTCTTCGTT CCACTGGACA CCCACTCCAA CGCATGCACA GACTCCCATC
    TTCCCCTCTG CTCCATTCGC CAAGCCTCCT TGCTCTTTCC AGAATGGCTT TAGTCCAGCA
    ATGTCTCCAT TTATTCCACC GACCCCCTTT CTCTCCTTTA CTCCCATCCA ACCACCTGCT
    GCTCTGGTCT ACTCGCAGCC TGCCGTCACC CCAGAGATGG CCAAGCTGTT TGACAAGATT
    GCCAGCACTT CGGAATACTT GAAGAACGGC AGGTCCTCTA GCATGGAAAC CGAATCGGCA
    AGTGTCAAGT CTCTGGAGCC CCTACCTCTG CCCTCAGAAC CTCCTAACAT CAGCCGATTT
    GAATGGCTGG ACTTGGACCC TCTCAACAAG CGCAAGGAGG TGGAGGTCGA GGAGACACCT
    GCTGTTTCAA GCTGTCCTTT TATTGAGGAG TCTGGGAAAG CTAAAGACCC GTGGGATGCG
    GTTCTTCAGG ATGAGCATGA AACTGTAGAT AATGGCAGTC CCACCGCAGA GGAAAAGAGC
    AAGGCAACTC AACCCAGAAG AGCATCCACA GGGGCGGCTG TAACAAAAAG CCAATCCCTC
    AGTATATCTG CAAACTCAAC GAATCAAAGC ACAGGCAAAC AGGTCAATAA GGGAAGCACA
    AATGTACTGT CCAAGTACTC TGCCTTACAG GAGAAGGAAG CCCAGAATCT AGAAGTTGTA
    GCATTCTGTG AAGACATTTC AAAACTGAGG TCAAAGTTCC CACACGATGA CCTGTCTACC
    AACCCTGGCT ATGTTCTCAG CCCTGTGATC ACGCAGAGGG ATGCAGGAGG GGACAATGGG
    GGCAGTGTGA AAGTGTCCAT TGAGATTTCT GATTCTCAAC AGCCTGTAAC GTTTACTTGC
    GATGTGACTT CTCCAGTGGA CTTGCTCATA ATGCAGGCTT TGTGTTGGGT TCATGATGAC
    CTGAACCAGG TGGACATCAA TAGCTACGTT CTCAAGGTCT GCGGACAGGA GGAGGTCCTT
    CAGAATAAAC ACAGTCTAGG CAGTCATGAG TACGTGCAGA ACTGCAGGAA GTGGGAATCA
    GAGATCAAAC TACAGCTCCT GTTTCTTAGC ACAATGAGAA GAGATCTGGC CCGAACTGCA
    GAAGATGACA TCTCGCCCAT TGATTTGGAG AAATACCTTG GTCTAGTGGA GAGGCCATTT
    AAAGAGGCAG TCACAAGAGA AGGCCTTGCT GAATATCTAG AAGGCTTTCA TAAGCAAGTC
    AATCTCTGCC TTCAGAATGA GAACAACCAA TATAAAACAG TGGACAATGT GGTTCAGTCT
    GTTAAGAATC TGTGCTGTGC TCTGGATGAA GTTGAGACAC CAGCCATCAC TGATGCCGTC
    AAGAGGCTCA AGCGCTCTGT CAACCTTCCT AGAACACGCT CACCAGAGCA CTATGCTGAA
    TTGCAGGCTG GTGCCACATC TTCCGGAGGT TCAGCTAATG GTTACTCTAG CCCTGTAGAG
    GAGAGTTTAG CAGTGCTCAC AGATGCTGTC TATGAACTCG CCAAGCTCTA CCTTCAGTCT
    TTCTGCCCGA TTAGCCCTGG TTTCATGATA GAAGAGCACA AAGAAGATGA GAGGGACAGC
    AAAGAGGCCT CGGGCACCAC TGAACACTTA CAGTTCACAC TGTTTGCCGT TCATGGCATT
    CCTGCTACCT GGGTCAGCAG TTTTGAAAAA TACTACCTGA TGTGTGCCCT GACCCATAAC
    AACAGGAATC TCTTCAAACC TGTCCAGTCC AAAAAAGTGG GAACATACAA GAGTTTCTTC
    TACCACATCA AATGGGATGA ATTAATAAAC TTCCCTATTT CAGTGTCGCT GTTACCACTG
    GAGGCTATGC TTAGTCTTTC TCTCTATGGG GTCCTCAGTC AGAATGCAAA CAACTCACCA
    GATTCCAACA AGCAACGGAA AGGTCCAGAA CTTTTGGGAA AAGTTTCCAT GCCTCTGTTT
    GACTTCAGGA GGGTTCTGTC CAGAGGCAGT AAACTGCTGA GTTTGTGGAC TTCTCCTCAA
    GCCCTTCAGC CTGGAGCTGC AGGCAAAGGG AGGAATCCTA CAGAAAAGAT CATACTGCAG
    GTTGACTTCC CCAGTCCAGC GGTGGACGTG CTCTATGTTG GTCCTCAGGA GAACGGCTGC
    CCCAATCCTC AGTCTCTAGA CCCACTTGAT CAAGACGATC GAAGTGAGAT AGAAAAGCTG
    TGCGCACGAG CATCTACTTT CGGACTGTCA CGGGCGGACA GACAGCTGCT TTGGGACCAG
    AGGTACTATT GTCGGGAATA TGAATACAGT TTGCCGAAAA TCCTGGCCAG CGCTCCCAGC
    TGGGACTGGG GCAGTATGGG GGAAATCCAC GCACTTCTGC ACCACTGGCC TGCTCTGTCA
    CCCGTTTCAG CTCTTGAGCT CCTTGAATCC AAGTTTGCCG ACACAGAGGT GAGGAAAGTG
    GCTGTGAGCT GGATCCAGAG CAGCAGTGAT GATGAGCTGG CTGATTATCT CCCCCAGCTA
    GTGCAGGCAG TGAAGTTCGA GTGTCACCTT AACAATGCCT TGGTGAAGTT TCTGCTGTCT
    CGAGCTCTGG GAAATGTCAA CATTGCACAT TATCTTTACT GGCTGCTGAG GGATGCAGTG
    CAGGACCCGG CGTTTGGTCA GCGTTATGAG CGGATCCTCT GTGTGCTGCT GTGTCTGTGT
    GGGACCGGAC TGAGGGCTGA GCTGGAGAAG CAGACCCGAC TTGTGCAGCT GCTTGGAGCT
    CTGGCTGAAA AGGTTCGGCA GGCGAGCAGC TCAACCCGAC AGGTGGTTCT CCTCGAGGGT
    CTAGAGAGGG TTCAGTCTTT CTTCCAGAAG AACAGCTGTC GACTCCCTAT TAGCCCCAGT
    CTGGTGGCAA AAGAGCTCAA TATCAAGGTC TGCTCCTTTT TCAATTCCAA CGCTGTACCT
    CTAAAGATCG CACTGGTTAA CGCAGATCCG CTGGGAGATG AGATTAACGT CATGTTTAAG
    GTTGGAGAAG ACCTGCGGCA GGACATGTTG GCACTACAGA TGATTCGGAT TATGGATCGG
    ATCTGGCTTC AGGAAGGACT CGACCTGCGT ATTGTTAACT TTAAATGCAT CTCTACTGGG
    AAAGATAAAG GCATGGTGGA GTTGGTACCA TCTTCTGAAA CGCTGAGAAA GATTCAGGTG
    GAGTATGGAG TGACGGGTTC CTTCAAGGAC AAACCTCTTG CTGAGTGGCT TCGCAAGTAT
    AACCCTGCTG AGGATGAGTA TGAGAAGGCG TCTGAGAATT TTATCTACAC CTGTGCTGGA
    TGCTGTGTGG CCACTTACAT CCTCGGCATC TGCGATCGTC ACAACGACAA CATCATGCTG
    CGCTCCACTG GCCACATGTT CCACATAGAC TTCGGGAAGT TCCTGGGTCA CGCACAAATG
    ATTGGGAGTT TTAAAAGGGA CCGAGCACCG TTTGTCTTAA CCTCTGACAT GGCTTATGTC
    ATCAATGGAG GCGAAAGACC CACTAGTCGC TTTCAGCTGT TCGTTGACCT TTGCTCGCAA
    GCCTACAACC TGATCCGCAA ACACTCCAAC CTCTTCCTCA ACCTGCTATC TCTGATGACA
    CAATCAGGTT TACCCGAGCT GACCGGAGTC CAAGATTTGA AGTATGTGTA TGATGCTCTT
    CAGCCTCAGA CTACAGATGC AGAGGCTACC ATTTTCTTCA CAAGGTTGAT TGAATCCAGT
    CTGGGCAGTG TGGCTACAAA GTTTAATTTC TTTATCCACA ATCTGGCTCA GTTGCGTTTC
    TCTGGCCTTC CCTCCAATGA TGAACCCATC CTGTCGTTTG CTCCTCGGAC ATACACTATG
    AAGCAAGACG GCAAGATTCG GGACGCCTCT GTGTTTTCAT TTCAAAAGAG ATATAACCCA
    GACAAGCACT ATACGTATGT CATTCGGATT CTAAGGGAAG GTCAGAGCGA GCCACAGTTT
    GTTTTCCGCA CATTTGATGA GTTTCAGGAA TTGCACAACA AATTGACCAT CCTTTTTCAG
    TTGTGGAAGC TGCCAGGGTT TCCCAGTAAG ATGGTGCTGG GACGCACACA CATCAAGGAT
    GTGGCATCCA AGAGAAAGGT TGAATTGAAT AGTTATGTGC ACAATCTGAT GAGGAGTTCA
    ACAGAGGTCG CCCAGTGTGA TCTGATCTAC ACCTTCTTCC ACCCTATCGC AAGAGATGAC
    AAGACTGAAG GGGTTGATAC CCTATCAAAA ACTCCAGACA TGCCTCCAGT GAGTCCCACC
    ACAGGCCGTG TGGAGGGTGA GGTGAAGCTG TCAGTGTCCT ACAGAAACAG CACACTTTTC
    ATCATGGTCA TGCACATTAA AGACCTGATG TCCAATGACG GAGCAGACCC AAACCCTTAT
    GTGAAAACAT ACCTTCTCCC AGACCCCCAC AAAACTTCCA AACGCAAAAC AAAGATTGCG
    AGGAAGACAA GGAACCCAAC ATTTAATGAA ATGCTGGTGT ATAGCGGTTA CAGTAAGGAA
    ACACTGAAGC AAAGGGAACT TCAACTCAGT GTGCTCAGTG CCGAGTCTCT GCGAGAGAAC
    TGCTATTTGG GAGGAATAAC CCTCTGCCTT AAAGACTTTG ACCTCAGCCG AGAGACAGTC
    AAGTGGTACA AACTCACCAC GGTGCCCTAC TTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23385
    Clone ID Related Accession (Same CDS sequence) XM_021467820.1 , XM_005159305.3 , XM_005159306.2 , XM_021467821.1
    Accession Version XM_005159305.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159305.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCCCACA TATCCAGTGG CAATGGGTTT AAGTTTGACA GGCCAGCCTC TCCGGGGGTG 
    GTCCGGCCAA AAGGAATTGT GGGGAAGGAA GAGGCTTTAC GCATGGAGGT AGAGGCCTTC
    GATAAAATTA AGCGCGAGAA AAGGCACACA CTCCCAGTCA CAGCCTCTTC TGTTAATCCC
    CTTCCCAATC CAACAGCGCA AACATCCAGC AGCCGGCCAG AGAAAGACCT TATTGTATTC
    TGCGAATCAG AAACACAGAA GAAGGAGCAA AAAGACAACT TTGAGGACAT CGATTTGGAG
    AACCTGACAA AGGAGGAGCT GGAAAAGCTG CTTCTGGATG ACAGTTTTGG TGCAAACAAA
    ATGACAAGAC CTTCCTCTTT GCTAGGTTGC AATCTCAGCG CATCATATCC TGGCGGACAT
    GCCTTCAACC CCTCTTCGTT CCACTGGACA CCCACTCCAA CGCATGCACA GACTCCCATC
    TTCCCCTCTG CTCCATTCGC CAAGCCTCCT TGCTCTTTCC AGAATGGCTT TAGTCCAGCA
    ATGTCTCCAT TTATTCCACC GACCCCCTTT CTCTCCTTTA CTCCCATCCA ACCACCTGCT
    GCTCTGGTCT ACTCGCAGCC TGCCGTCACC CCAGAGATGG CCAAGCTGTT TGACAAGATT
    GCCAGCACTT CGGAATACTT GAAGAACGGC AGGTCCTCTA GCATGGAAAC CGAATCGGCA
    AGTGTCAAGT CTCTGGAGCC CCTACCTCTG CCCTCAGAAC CTCCTAACAT CAGCCGATTT
    GAATGGCTGG ACTTGGACCC TCTCAACAAG CGCAAGGAGG TGGAGGTCGA GGAGACACCT
    GCTGTTTCAA GCTGTCCTTT TATTGAGGAG TCTGGGAAAG CTAAAGACCC GTGGGATGCG
    GTTCTTCAGG ATGAGCATGA AACTGTAGAT AATGGCAGTC CCACCGCAGA GGAAAAGAGC
    AAGGCAACTC AACCCAGAAG AGCATCCACA GGGGCGGCTG TAACAAAAAG CCAATCCCTC
    AGTATATCTG CAAACTCAAC GAATCAAAGC ACAGGCAAAC AGGTCAATAA GGGAAGCACA
    AATGTACTGT CCAAGTACTC TGCCTTACAG GAGAAGGAAG CCCAGAATCT AGAAGTTGTA
    GCATTCTGTG AAGACATTTC AAAACTGAGG TCAAAGTTCC CACACGATGA CCTGTCTACC
    AACCCTGGCT ATGTTCTCAG CCCTGTGATC ACGCAGAGGG ATGCAGGAGG GGACAATGGG
    GGCAGTGTGA AAGTGTCCAT TGAGATTTCT GATTCTCAAC AGCCTGTAAC GTTTACTTGC
    GATGTGACTT CTCCAGTGGA CTTGCTCATA ATGCAGGCTT TGTGTTGGGT TCATGATGAC
    CTGAACCAGG TGGACATCAA TAGCTACGTT CTCAAGGTCT GCGGACAGGA GGAGGTCCTT
    CAGAATAAAC ACAGTCTAGG CAGTCATGAG TACGTGCAGA ACTGCAGGAA GTGGGAATCA
    GAGATCAAAC TACAGCTCCT GTTTCTTAGC ACAATGAGAA GAGATCTGGC CCGAACTGCA
    GAAGATGACA TCTCGCCCAT TGATTTGGAG AAATACCTTG GTCTAGTGGA GAGGCCATTT
    AAAGAGGCAG TCACAAGAGA AGGCCTTGCT GAATATCTAG AAGGCTTTCA TAAGCAAGTC
    AATCTCTGCC TTCAGAATGA GAACAACCAA TATAAAACAG TGGACAATGT GGTTCAGTCT
    GTTAAGAATC TGTGCTGTGC TCTGGATGAA GTTGAGACAC CAGCCATCAC TGATGCCGTC
    AAGAGGCTCA AGCGCTCTGT CAACCTTCCT AGAACACGCT CACCAGAGCA CTATGCTGAA
    TTGCAGGCTG GTGCCACATC TTCCGGAGGT TCAGCTAATG GTTACTCTAG CCCTGTAGAG
    GAGAGTTTAG CAGTGCTCAC AGATGCTGTC TATGAACTCG CCAAGCTCTA CCTTCAGTCT
    TTCTGCCCGA TTAGCCCTGG TTTCATGATA GAAGAGCACA AAGAAGATGA GAGGGACAGC
    AAAGAGGCCT CGGGCACCAC TGAACACTTA CAGTTCACAC TGTTTGCCGT TCATGGCATT
    CCTGCTACCT GGGTCAGCAG TTTTGAAAAA TACTACCTGA TGTGTGCCCT GACCCATAAC
    AACAGGAATC TCTTCAAACC TGTCCAGTCC AAAAAAGTGG GAACATACAA GAGTTTCTTC
    TACCACATCA AATGGGATGA ATTAATAAAC TTCCCTATTT CAGTGTCGCT GTTACCACTG
    GAGGCTATGC TTAGTCTTTC TCTCTATGGG GTCCTCAGTC AGAATGCAAA CAACTCACCA
    GATTCCAACA AGCAACGGAA AGGTCCAGAA CTTTTGGGAA AAGTTTCCAT GCCTCTGTTT
    GACTTCAGGA GGGTTCTGTC CAGAGGCAGT AAACTGCTGA GTTTGTGGAC TTCTCCTCAA
    GCCCTTCAGC CTGGAGCTGC AGGCAAAGGG AGGAATCCTA CAGAAAAGAT CATACTGCAG
    GTTGACTTCC CCAGTCCAGC GGTGGACGTG CTCTATGTTG GTCCTCAGGA GAACGGCTGC
    CCCAATCCTC AGTCTCTAGA CCCACTTGAT CAAGACGATC GAAGTGAGAT AGAAAAGCTG
    TGCGCACGAG CATCTACTTT CGGACTGTCA CGGGCGGACA GACAGCTGCT TTGGGACCAG
    AGGTACTATT GTCGGGAATA TGAATACAGT TTGCCGAAAA TCCTGGCCAG CGCTCCCAGC
    TGGGACTGGG GCAGTATGGG GGAAATCCAC GCACTTCTGC ACCACTGGCC TGCTCTGTCA
    CCCGTTTCAG CTCTTGAGCT CCTTGAATCC AAGTTTGCCG ACACAGAGGT GAGGAAAGTG
    GCTGTGAGCT GGATCCAGAG CAGCAGTGAT GATGAGCTGG CTGATTATCT CCCCCAGCTA
    GTGCAGGCAG TGAAGTTCGA GTGTCACCTT AACAATGCCT TGGTGAAGTT TCTGCTGTCT
    CGAGCTCTGG GAAATGTCAA CATTGCACAT TATCTTTACT GGCTGCTGAG GGATGCAGTG
    CAGGACCCGG CGTTTGGTCA GCGTTATGAG CGGATCCTCT GTGTGCTGCT GTGTCTGTGT
    GGGACCGGAC TGAGGGCTGA GCTGGAGAAG CAGACCCGAC TTGTGCAGCT GCTTGGAGCT
    CTGGCTGAAA AGGTTCGGCA GGCGAGCAGC TCAACCCGAC AGGTGGTTCT CCTCGAGGGT
    CTAGAGAGGG TTCAGTCTTT CTTCCAGAAG AACAGCTGTC GACTCCCTAT TAGCCCCAGT
    CTGGTGGCAA AAGAGCTCAA TATCAAGGTC TGCTCCTTTT TCAATTCCAA CGCTGTACCT
    CTAAAGATCG CACTGGTTAA CGCAGATCCG CTGGGAGATG AGATTAACGT CATGTTTAAG
    GTTGGAGAAG ACCTGCGGCA GGACATGTTG GCACTACAGA TGATTCGGAT TATGGATCGG
    ATCTGGCTTC AGGAAGGACT CGACCTGCGT ATTGTTAACT TTAAATGCAT CTCTACTGGG
    AAAGATAAAG GCATGGTGGA GTTGGTACCA TCTTCTGAAA CGCTGAGAAA GATTCAGGTG
    GAGTATGGAG TGACGGGTTC CTTCAAGGAC AAACCTCTTG CTGAGTGGCT TCGCAAGTAT
    AACCCTGCTG AGGATGAGTA TGAGAAGGCG TCTGAGAATT TTATCTACAC CTGTGCTGGA
    TGCTGTGTGG CCACTTACAT CCTCGGCATC TGCGATCGTC ACAACGACAA CATCATGCTG
    CGCTCCACTG GCCACATGTT CCACATAGAC TTCGGGAAGT TCCTGGGTCA CGCACAAATG
    ATTGGGAGTT TTAAAAGGGA CCGAGCACCG TTTGTCTTAA CCTCTGACAT GGCTTATGTC
    ATCAATGGAG GCGAAAGACC CACTAGTCGC TTTCAGCTGT TCGTTGACCT TTGCTCGCAA
    GCCTACAACC TGATCCGCAA ACACTCCAAC CTCTTCCTCA ACCTGCTATC TCTGATGACA
    CAATCAGGTT TACCCGAGCT GACCGGAGTC CAAGATTTGA AGTATGTGTA TGATGCTCTT
    CAGCCTCAGA CTACAGATGC AGAGGCTACC ATTTTCTTCA CAAGGTTGAT TGAATCCAGT
    CTGGGCAGTG TGGCTACAAA GTTTAATTTC TTTATCCACA ATCTGGCTCA GTTGCGTTTC
    TCTGGCCTTC CCTCCAATGA TGAACCCATC CTGTCGTTTG CTCCTCGGAC ATACACTATG
    AAGCAAGACG GCAAGATTCG GGACGCCTCT GTGTTTTCAT TTCAAAAGAG ATATAACCCA
    GACAAGCACT ATACGTATGT CATTCGGATT CTAAGGGAAG GTCAGAGCGA GCCACAGTTT
    GTTTTCCGCA CATTTGATGA GTTTCAGGAA TTGCACAACA AATTGACCAT CCTTTTTCAG
    TTGTGGAAGC TGCCAGGGTT TCCCAGTAAG ATGGTGCTGG GACGCACACA CATCAAGGAT
    GTGGCATCCA AGAGAAAGGT TGAATTGAAT AGTTATGTGC ACAATCTGAT GAGGAGTTCA
    ACAGAGGTCG CCCAGTGTGA TCTGATCTAC ACCTTCTTCC ACCCTATCGC AAGAGATGAC
    AAGACTGAAG GGGTTGATAC CCTATCAAAA ACTCCAGACA TGCCTCCAGT GAGTCCCACC
    ACAGGCCGTG TGGAGGGTGA GGTGAAGCTG TCAGTGTCCT ACAGAAACAG CACACTTTTC
    ATCATGGTCA TGCACATTAA AGACCTGATG TCCAATGACG GAGCAGACCC AAACCCTTAT
    GTGAAAACAT ACCTTCTCCC AGACCCCCAC AAAACTTCCA AACGCAAAAC AAAGATTGCG
    AGGAAGACAA GGAACCCAAC ATTTAATGAA ATGCTGGTGT ATAGCGGTTA CAGTAAGGAA
    ACACTGAAGC AAAGGGAACT TCAACTCAGT GTGCTCAGTG CCGAGTCTCT GCGAGAGAAC
    TGCTATTTGG GAGGAATAAC CCTCTGCCTT AAAGACTTTG ACCTCAGCCG AGAGACAGTC
    AAGTGGTACA AACTCACCAC GGTGCCCTAC TTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005159362.1
    CDS159..5234
    Translation

    Target ORF information:

    RefSeq Version XM_005159305.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha (pik3c2a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005159305.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCCCACA TATCCAGTGG CAATGGGTTT AAGTTTGACA GGCCAGCCTC TCCGGGGGTG 
    GTCCGGCCAA AAGGAATTGT GGGGAAGGAA GAGGCTTTAC GCATGGAGGT AGAGGCCTTC
    GATAAAATTA AGCGCGAGAA AAGGCACACA CTCCCAGTCA CAGCCTCTTC TGTTAATCCC
    CTTCCCAATC CAACAGCGCA AACATCCAGC AGCCGGCCAG AGAAAGACCT TATTGTATTC
    TGCGAATCAG AAACACAGAA GAAGGAGCAA AAAGACAACT TTGAGGACAT CGATTTGGAG
    AACCTGACAA AGGAGGAGCT GGAAAAGCTG CTTCTGGATG ACAGTTTTGG TGCAAACAAA
    ATGACAAGAC CTTCCTCTTT GCTAGGTTGC AATCTCAGCG CATCATATCC TGGCGGACAT
    GCCTTCAACC CCTCTTCGTT CCACTGGACA CCCACTCCAA CGCATGCACA GACTCCCATC
    TTCCCCTCTG CTCCATTCGC CAAGCCTCCT TGCTCTTTCC AGAATGGCTT TAGTCCAGCA
    ATGTCTCCAT TTATTCCACC GACCCCCTTT CTCTCCTTTA CTCCCATCCA ACCACCTGCT
    GCTCTGGTCT ACTCGCAGCC TGCCGTCACC CCAGAGATGG CCAAGCTGTT TGACAAGATT
    GCCAGCACTT CGGAATACTT GAAGAACGGC AGGTCCTCTA GCATGGAAAC CGAATCGGCA
    AGTGTCAAGT CTCTGGAGCC CCTACCTCTG CCCTCAGAAC CTCCTAACAT CAGCCGATTT
    GAATGGCTGG ACTTGGACCC TCTCAACAAG CGCAAGGAGG TGGAGGTCGA GGAGACACCT
    GCTGTTTCAA GCTGTCCTTT TATTGAGGAG TCTGGGAAAG CTAAAGACCC GTGGGATGCG
    GTTCTTCAGG ATGAGCATGA AACTGTAGAT AATGGCAGTC CCACCGCAGA GGAAAAGAGC
    AAGGCAACTC AACCCAGAAG AGCATCCACA GGGGCGGCTG TAACAAAAAG CCAATCCCTC
    AGTATATCTG CAAACTCAAC GAATCAAAGC ACAGGCAAAC AGGTCAATAA GGGAAGCACA
    AATGTACTGT CCAAGTACTC TGCCTTACAG GAGAAGGAAG CCCAGAATCT AGAAGTTGTA
    GCATTCTGTG AAGACATTTC AAAACTGAGG TCAAAGTTCC CACACGATGA CCTGTCTACC
    AACCCTGGCT ATGTTCTCAG CCCTGTGATC ACGCAGAGGG ATGCAGGAGG GGACAATGGG
    GGCAGTGTGA AAGTGTCCAT TGAGATTTCT GATTCTCAAC AGCCTGTAAC GTTTACTTGC
    GATGTGACTT CTCCAGTGGA CTTGCTCATA ATGCAGGCTT TGTGTTGGGT TCATGATGAC
    CTGAACCAGG TGGACATCAA TAGCTACGTT CTCAAGGTCT GCGGACAGGA GGAGGTCCTT
    CAGAATAAAC ACAGTCTAGG CAGTCATGAG TACGTGCAGA ACTGCAGGAA GTGGGAATCA
    GAGATCAAAC TACAGCTCCT GTTTCTTAGC ACAATGAGAA GAGATCTGGC CCGAACTGCA
    GAAGATGACA TCTCGCCCAT TGATTTGGAG AAATACCTTG GTCTAGTGGA GAGGCCATTT
    AAAGAGGCAG TCACAAGAGA AGGCCTTGCT GAATATCTAG AAGGCTTTCA TAAGCAAGTC
    AATCTCTGCC TTCAGAATGA GAACAACCAA TATAAAACAG TGGACAATGT GGTTCAGTCT
    GTTAAGAATC TGTGCTGTGC TCTGGATGAA GTTGAGACAC CAGCCATCAC TGATGCCGTC
    AAGAGGCTCA AGCGCTCTGT CAACCTTCCT AGAACACGCT CACCAGAGCA CTATGCTGAA
    TTGCAGGCTG GTGCCACATC TTCCGGAGGT TCAGCTAATG GTTACTCTAG CCCTGTAGAG
    GAGAGTTTAG CAGTGCTCAC AGATGCTGTC TATGAACTCG CCAAGCTCTA CCTTCAGTCT
    TTCTGCCCGA TTAGCCCTGG TTTCATGATA GAAGAGCACA AAGAAGATGA GAGGGACAGC
    AAAGAGGCCT CGGGCACCAC TGAACACTTA CAGTTCACAC TGTTTGCCGT TCATGGCATT
    CCTGCTACCT GGGTCAGCAG TTTTGAAAAA TACTACCTGA TGTGTGCCCT GACCCATAAC
    AACAGGAATC TCTTCAAACC TGTCCAGTCC AAAAAAGTGG GAACATACAA GAGTTTCTTC
    TACCACATCA AATGGGATGA ATTAATAAAC TTCCCTATTT CAGTGTCGCT GTTACCACTG
    GAGGCTATGC TTAGTCTTTC TCTCTATGGG GTCCTCAGTC AGAATGCAAA CAACTCACCA
    GATTCCAACA AGCAACGGAA AGGTCCAGAA CTTTTGGGAA AAGTTTCCAT GCCTCTGTTT
    GACTTCAGGA GGGTTCTGTC CAGAGGCAGT AAACTGCTGA GTTTGTGGAC TTCTCCTCAA
    GCCCTTCAGC CTGGAGCTGC AGGCAAAGGG AGGAATCCTA CAGAAAAGAT CATACTGCAG
    GTTGACTTCC CCAGTCCAGC GGTGGACGTG CTCTATGTTG GTCCTCAGGA GAACGGCTGC
    CCCAATCCTC AGTCTCTAGA CCCACTTGAT CAAGACGATC GAAGTGAGAT AGAAAAGCTG
    TGCGCACGAG CATCTACTTT CGGACTGTCA CGGGCGGACA GACAGCTGCT TTGGGACCAG
    AGGTACTATT GTCGGGAATA TGAATACAGT TTGCCGAAAA TCCTGGCCAG CGCTCCCAGC
    TGGGACTGGG GCAGTATGGG GGAAATCCAC GCACTTCTGC ACCACTGGCC TGCTCTGTCA
    CCCGTTTCAG CTCTTGAGCT CCTTGAATCC AAGTTTGCCG ACACAGAGGT GAGGAAAGTG
    GCTGTGAGCT GGATCCAGAG CAGCAGTGAT GATGAGCTGG CTGATTATCT CCCCCAGCTA
    GTGCAGGCAG TGAAGTTCGA GTGTCACCTT AACAATGCCT TGGTGAAGTT TCTGCTGTCT
    CGAGCTCTGG GAAATGTCAA CATTGCACAT TATCTTTACT GGCTGCTGAG GGATGCAGTG
    CAGGACCCGG CGTTTGGTCA GCGTTATGAG CGGATCCTCT GTGTGCTGCT GTGTCTGTGT
    GGGACCGGAC TGAGGGCTGA GCTGGAGAAG CAGACCCGAC TTGTGCAGCT GCTTGGAGCT
    CTGGCTGAAA AGGTTCGGCA GGCGAGCAGC TCAACCCGAC AGGTGGTTCT CCTCGAGGGT
    CTAGAGAGGG TTCAGTCTTT CTTCCAGAAG AACAGCTGTC GACTCCCTAT TAGCCCCAGT
    CTGGTGGCAA AAGAGCTCAA TATCAAGGTC TGCTCCTTTT TCAATTCCAA CGCTGTACCT
    CTAAAGATCG CACTGGTTAA CGCAGATCCG CTGGGAGATG AGATTAACGT CATGTTTAAG
    GTTGGAGAAG ACCTGCGGCA GGACATGTTG GCACTACAGA TGATTCGGAT TATGGATCGG
    ATCTGGCTTC AGGAAGGACT CGACCTGCGT ATTGTTAACT TTAAATGCAT CTCTACTGGG
    AAAGATAAAG GCATGGTGGA GTTGGTACCA TCTTCTGAAA CGCTGAGAAA GATTCAGGTG
    GAGTATGGAG TGACGGGTTC CTTCAAGGAC AAACCTCTTG CTGAGTGGCT TCGCAAGTAT
    AACCCTGCTG AGGATGAGTA TGAGAAGGCG TCTGAGAATT TTATCTACAC CTGTGCTGGA
    TGCTGTGTGG CCACTTACAT CCTCGGCATC TGCGATCGTC ACAACGACAA CATCATGCTG
    CGCTCCACTG GCCACATGTT CCACATAGAC TTCGGGAAGT TCCTGGGTCA CGCACAAATG
    ATTGGGAGTT TTAAAAGGGA CCGAGCACCG TTTGTCTTAA CCTCTGACAT GGCTTATGTC
    ATCAATGGAG GCGAAAGACC CACTAGTCGC TTTCAGCTGT TCGTTGACCT TTGCTCGCAA
    GCCTACAACC TGATCCGCAA ACACTCCAAC CTCTTCCTCA ACCTGCTATC TCTGATGACA
    CAATCAGGTT TACCCGAGCT GACCGGAGTC CAAGATTTGA AGTATGTGTA TGATGCTCTT
    CAGCCTCAGA CTACAGATGC AGAGGCTACC ATTTTCTTCA CAAGGTTGAT TGAATCCAGT
    CTGGGCAGTG TGGCTACAAA GTTTAATTTC TTTATCCACA ATCTGGCTCA GTTGCGTTTC
    TCTGGCCTTC CCTCCAATGA TGAACCCATC CTGTCGTTTG CTCCTCGGAC ATACACTATG
    AAGCAAGACG GCAAGATTCG GGACGCCTCT GTGTTTTCAT TTCAAAAGAG ATATAACCCA
    GACAAGCACT ATACGTATGT CATTCGGATT CTAAGGGAAG GTCAGAGCGA GCCACAGTTT
    GTTTTCCGCA CATTTGATGA GTTTCAGGAA TTGCACAACA AATTGACCAT CCTTTTTCAG
    TTGTGGAAGC TGCCAGGGTT TCCCAGTAAG ATGGTGCTGG GACGCACACA CATCAAGGAT
    GTGGCATCCA AGAGAAAGGT TGAATTGAAT AGTTATGTGC ACAATCTGAT GAGGAGTTCA
    ACAGAGGTCG CCCAGTGTGA TCTGATCTAC ACCTTCTTCC ACCCTATCGC AAGAGATGAC
    AAGACTGAAG GGGTTGATAC CCTATCAAAA ACTCCAGACA TGCCTCCAGT GAGTCCCACC
    ACAGGCCGTG TGGAGGGTGA GGTGAAGCTG TCAGTGTCCT ACAGAAACAG CACACTTTTC
    ATCATGGTCA TGCACATTAA AGACCTGATG TCCAATGACG GAGCAGACCC AAACCCTTAT
    GTGAAAACAT ACCTTCTCCC AGACCCCCAC AAAACTTCCA AACGCAAAAC AAAGATTGCG
    AGGAAGACAA GGAACCCAAC ATTTAATGAA ATGCTGGTGT ATAGCGGTTA CAGTAAGGAA
    ACACTGAAGC AAAGGGAACT TCAACTCAGT GTGCTCAGTG CCGAGTCTCT GCGAGAGAAC
    TGCTATTTGG GAGGAATAAC CCTCTGCCTT AAAGACTTTG ACCTCAGCCG AGAGACAGTC
    AAGTGGTACA AACTCACCAC GGTGCCCTAC TTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23385
    Clone ID Related Accession (Same CDS sequence) XM_021467820.1 , XM_005159305.3 , XM_005159306.2 , XM_021467821.1
    Accession Version XM_005159306.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159306.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCCCACA TATCCAGTGG CAATGGGTTT AAGTTTGACA GGCCAGCCTC TCCGGGGGTG 
    GTCCGGCCAA AAGGAATTGT GGGGAAGGAA GAGGCTTTAC GCATGGAGGT AGAGGCCTTC
    GATAAAATTA AGCGCGAGAA AAGGCACACA CTCCCAGTCA CAGCCTCTTC TGTTAATCCC
    CTTCCCAATC CAACAGCGCA AACATCCAGC AGCCGGCCAG AGAAAGACCT TATTGTATTC
    TGCGAATCAG AAACACAGAA GAAGGAGCAA AAAGACAACT TTGAGGACAT CGATTTGGAG
    AACCTGACAA AGGAGGAGCT GGAAAAGCTG CTTCTGGATG ACAGTTTTGG TGCAAACAAA
    ATGACAAGAC CTTCCTCTTT GCTAGGTTGC AATCTCAGCG CATCATATCC TGGCGGACAT
    GCCTTCAACC CCTCTTCGTT CCACTGGACA CCCACTCCAA CGCATGCACA GACTCCCATC
    TTCCCCTCTG CTCCATTCGC CAAGCCTCCT TGCTCTTTCC AGAATGGCTT TAGTCCAGCA
    ATGTCTCCAT TTATTCCACC GACCCCCTTT CTCTCCTTTA CTCCCATCCA ACCACCTGCT
    GCTCTGGTCT ACTCGCAGCC TGCCGTCACC CCAGAGATGG CCAAGCTGTT TGACAAGATT
    GCCAGCACTT CGGAATACTT GAAGAACGGC AGGTCCTCTA GCATGGAAAC CGAATCGGCA
    AGTGTCAAGT CTCTGGAGCC CCTACCTCTG CCCTCAGAAC CTCCTAACAT CAGCCGATTT
    GAATGGCTGG ACTTGGACCC TCTCAACAAG CGCAAGGAGG TGGAGGTCGA GGAGACACCT
    GCTGTTTCAA GCTGTCCTTT TATTGAGGAG TCTGGGAAAG CTAAAGACCC GTGGGATGCG
    GTTCTTCAGG ATGAGCATGA AACTGTAGAT AATGGCAGTC CCACCGCAGA GGAAAAGAGC
    AAGGCAACTC AACCCAGAAG AGCATCCACA GGGGCGGCTG TAACAAAAAG CCAATCCCTC
    AGTATATCTG CAAACTCAAC GAATCAAAGC ACAGGCAAAC AGGTCAATAA GGGAAGCACA
    AATGTACTGT CCAAGTACTC TGCCTTACAG GAGAAGGAAG CCCAGAATCT AGAAGTTGTA
    GCATTCTGTG AAGACATTTC AAAACTGAGG TCAAAGTTCC CACACGATGA CCTGTCTACC
    AACCCTGGCT ATGTTCTCAG CCCTGTGATC ACGCAGAGGG ATGCAGGAGG GGACAATGGG
    GGCAGTGTGA AAGTGTCCAT TGAGATTTCT GATTCTCAAC AGCCTGTAAC GTTTACTTGC
    GATGTGACTT CTCCAGTGGA CTTGCTCATA ATGCAGGCTT TGTGTTGGGT TCATGATGAC
    CTGAACCAGG TGGACATCAA TAGCTACGTT CTCAAGGTCT GCGGACAGGA GGAGGTCCTT
    CAGAATAAAC ACAGTCTAGG CAGTCATGAG TACGTGCAGA ACTGCAGGAA GTGGGAATCA
    GAGATCAAAC TACAGCTCCT GTTTCTTAGC ACAATGAGAA GAGATCTGGC CCGAACTGCA
    GAAGATGACA TCTCGCCCAT TGATTTGGAG AAATACCTTG GTCTAGTGGA GAGGCCATTT
    AAAGAGGCAG TCACAAGAGA AGGCCTTGCT GAATATCTAG AAGGCTTTCA TAAGCAAGTC
    AATCTCTGCC TTCAGAATGA GAACAACCAA TATAAAACAG TGGACAATGT GGTTCAGTCT
    GTTAAGAATC TGTGCTGTGC TCTGGATGAA GTTGAGACAC CAGCCATCAC TGATGCCGTC
    AAGAGGCTCA AGCGCTCTGT CAACCTTCCT AGAACACGCT CACCAGAGCA CTATGCTGAA
    TTGCAGGCTG GTGCCACATC TTCCGGAGGT TCAGCTAATG GTTACTCTAG CCCTGTAGAG
    GAGAGTTTAG CAGTGCTCAC AGATGCTGTC TATGAACTCG CCAAGCTCTA CCTTCAGTCT
    TTCTGCCCGA TTAGCCCTGG TTTCATGATA GAAGAGCACA AAGAAGATGA GAGGGACAGC
    AAAGAGGCCT CGGGCACCAC TGAACACTTA CAGTTCACAC TGTTTGCCGT TCATGGCATT
    CCTGCTACCT GGGTCAGCAG TTTTGAAAAA TACTACCTGA TGTGTGCCCT GACCCATAAC
    AACAGGAATC TCTTCAAACC TGTCCAGTCC AAAAAAGTGG GAACATACAA GAGTTTCTTC
    TACCACATCA AATGGGATGA ATTAATAAAC TTCCCTATTT CAGTGTCGCT GTTACCACTG
    GAGGCTATGC TTAGTCTTTC TCTCTATGGG GTCCTCAGTC AGAATGCAAA CAACTCACCA
    GATTCCAACA AGCAACGGAA AGGTCCAGAA CTTTTGGGAA AAGTTTCCAT GCCTCTGTTT
    GACTTCAGGA GGGTTCTGTC CAGAGGCAGT AAACTGCTGA GTTTGTGGAC TTCTCCTCAA
    GCCCTTCAGC CTGGAGCTGC AGGCAAAGGG AGGAATCCTA CAGAAAAGAT CATACTGCAG
    GTTGACTTCC CCAGTCCAGC GGTGGACGTG CTCTATGTTG GTCCTCAGGA GAACGGCTGC
    CCCAATCCTC AGTCTCTAGA CCCACTTGAT CAAGACGATC GAAGTGAGAT AGAAAAGCTG
    TGCGCACGAG CATCTACTTT CGGACTGTCA CGGGCGGACA GACAGCTGCT TTGGGACCAG
    AGGTACTATT GTCGGGAATA TGAATACAGT TTGCCGAAAA TCCTGGCCAG CGCTCCCAGC
    TGGGACTGGG GCAGTATGGG GGAAATCCAC GCACTTCTGC ACCACTGGCC TGCTCTGTCA
    CCCGTTTCAG CTCTTGAGCT CCTTGAATCC AAGTTTGCCG ACACAGAGGT GAGGAAAGTG
    GCTGTGAGCT GGATCCAGAG CAGCAGTGAT GATGAGCTGG CTGATTATCT CCCCCAGCTA
    GTGCAGGCAG TGAAGTTCGA GTGTCACCTT AACAATGCCT TGGTGAAGTT TCTGCTGTCT
    CGAGCTCTGG GAAATGTCAA CATTGCACAT TATCTTTACT GGCTGCTGAG GGATGCAGTG
    CAGGACCCGG CGTTTGGTCA GCGTTATGAG CGGATCCTCT GTGTGCTGCT GTGTCTGTGT
    GGGACCGGAC TGAGGGCTGA GCTGGAGAAG CAGACCCGAC TTGTGCAGCT GCTTGGAGCT
    CTGGCTGAAA AGGTTCGGCA GGCGAGCAGC TCAACCCGAC AGGTGGTTCT CCTCGAGGGT
    CTAGAGAGGG TTCAGTCTTT CTTCCAGAAG AACAGCTGTC GACTCCCTAT TAGCCCCAGT
    CTGGTGGCAA AAGAGCTCAA TATCAAGGTC TGCTCCTTTT TCAATTCCAA CGCTGTACCT
    CTAAAGATCG CACTGGTTAA CGCAGATCCG CTGGGAGATG AGATTAACGT CATGTTTAAG
    GTTGGAGAAG ACCTGCGGCA GGACATGTTG GCACTACAGA TGATTCGGAT TATGGATCGG
    ATCTGGCTTC AGGAAGGACT CGACCTGCGT ATTGTTAACT TTAAATGCAT CTCTACTGGG
    AAAGATAAAG GCATGGTGGA GTTGGTACCA TCTTCTGAAA CGCTGAGAAA GATTCAGGTG
    GAGTATGGAG TGACGGGTTC CTTCAAGGAC AAACCTCTTG CTGAGTGGCT TCGCAAGTAT
    AACCCTGCTG AGGATGAGTA TGAGAAGGCG TCTGAGAATT TTATCTACAC CTGTGCTGGA
    TGCTGTGTGG CCACTTACAT CCTCGGCATC TGCGATCGTC ACAACGACAA CATCATGCTG
    CGCTCCACTG GCCACATGTT CCACATAGAC TTCGGGAAGT TCCTGGGTCA CGCACAAATG
    ATTGGGAGTT TTAAAAGGGA CCGAGCACCG TTTGTCTTAA CCTCTGACAT GGCTTATGTC
    ATCAATGGAG GCGAAAGACC CACTAGTCGC TTTCAGCTGT TCGTTGACCT TTGCTCGCAA
    GCCTACAACC TGATCCGCAA ACACTCCAAC CTCTTCCTCA ACCTGCTATC TCTGATGACA
    CAATCAGGTT TACCCGAGCT GACCGGAGTC CAAGATTTGA AGTATGTGTA TGATGCTCTT
    CAGCCTCAGA CTACAGATGC AGAGGCTACC ATTTTCTTCA CAAGGTTGAT TGAATCCAGT
    CTGGGCAGTG TGGCTACAAA GTTTAATTTC TTTATCCACA ATCTGGCTCA GTTGCGTTTC
    TCTGGCCTTC CCTCCAATGA TGAACCCATC CTGTCGTTTG CTCCTCGGAC ATACACTATG
    AAGCAAGACG GCAAGATTCG GGACGCCTCT GTGTTTTCAT TTCAAAAGAG ATATAACCCA
    GACAAGCACT ATACGTATGT CATTCGGATT CTAAGGGAAG GTCAGAGCGA GCCACAGTTT
    GTTTTCCGCA CATTTGATGA GTTTCAGGAA TTGCACAACA AATTGACCAT CCTTTTTCAG
    TTGTGGAAGC TGCCAGGGTT TCCCAGTAAG ATGGTGCTGG GACGCACACA CATCAAGGAT
    GTGGCATCCA AGAGAAAGGT TGAATTGAAT AGTTATGTGC ACAATCTGAT GAGGAGTTCA
    ACAGAGGTCG CCCAGTGTGA TCTGATCTAC ACCTTCTTCC ACCCTATCGC AAGAGATGAC
    AAGACTGAAG GGGTTGATAC CCTATCAAAA ACTCCAGACA TGCCTCCAGT GAGTCCCACC
    ACAGGCCGTG TGGAGGGTGA GGTGAAGCTG TCAGTGTCCT ACAGAAACAG CACACTTTTC
    ATCATGGTCA TGCACATTAA AGACCTGATG TCCAATGACG GAGCAGACCC AAACCCTTAT
    GTGAAAACAT ACCTTCTCCC AGACCCCCAC AAAACTTCCA AACGCAAAAC AAAGATTGCG
    AGGAAGACAA GGAACCCAAC ATTTAATGAA ATGCTGGTGT ATAGCGGTTA CAGTAAGGAA
    ACACTGAAGC AAAGGGAACT TCAACTCAGT GTGCTCAGTG CCGAGTCTCT GCGAGAGAAC
    TGCTATTTGG GAGGAATAAC CCTCTGCCTT AAAGACTTTG ACCTCAGCCG AGAGACAGTC
    AAGTGGTACA AACTCACCAC GGTGCCCTAC TTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005159363.1
    CDS306..5381
    Translation

    Target ORF information:

    RefSeq Version XM_005159306.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha (pik3c2a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005159306.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCCCACA TATCCAGTGG CAATGGGTTT AAGTTTGACA GGCCAGCCTC TCCGGGGGTG 
    GTCCGGCCAA AAGGAATTGT GGGGAAGGAA GAGGCTTTAC GCATGGAGGT AGAGGCCTTC
    GATAAAATTA AGCGCGAGAA AAGGCACACA CTCCCAGTCA CAGCCTCTTC TGTTAATCCC
    CTTCCCAATC CAACAGCGCA AACATCCAGC AGCCGGCCAG AGAAAGACCT TATTGTATTC
    TGCGAATCAG AAACACAGAA GAAGGAGCAA AAAGACAACT TTGAGGACAT CGATTTGGAG
    AACCTGACAA AGGAGGAGCT GGAAAAGCTG CTTCTGGATG ACAGTTTTGG TGCAAACAAA
    ATGACAAGAC CTTCCTCTTT GCTAGGTTGC AATCTCAGCG CATCATATCC TGGCGGACAT
    GCCTTCAACC CCTCTTCGTT CCACTGGACA CCCACTCCAA CGCATGCACA GACTCCCATC
    TTCCCCTCTG CTCCATTCGC CAAGCCTCCT TGCTCTTTCC AGAATGGCTT TAGTCCAGCA
    ATGTCTCCAT TTATTCCACC GACCCCCTTT CTCTCCTTTA CTCCCATCCA ACCACCTGCT
    GCTCTGGTCT ACTCGCAGCC TGCCGTCACC CCAGAGATGG CCAAGCTGTT TGACAAGATT
    GCCAGCACTT CGGAATACTT GAAGAACGGC AGGTCCTCTA GCATGGAAAC CGAATCGGCA
    AGTGTCAAGT CTCTGGAGCC CCTACCTCTG CCCTCAGAAC CTCCTAACAT CAGCCGATTT
    GAATGGCTGG ACTTGGACCC TCTCAACAAG CGCAAGGAGG TGGAGGTCGA GGAGACACCT
    GCTGTTTCAA GCTGTCCTTT TATTGAGGAG TCTGGGAAAG CTAAAGACCC GTGGGATGCG
    GTTCTTCAGG ATGAGCATGA AACTGTAGAT AATGGCAGTC CCACCGCAGA GGAAAAGAGC
    AAGGCAACTC AACCCAGAAG AGCATCCACA GGGGCGGCTG TAACAAAAAG CCAATCCCTC
    AGTATATCTG CAAACTCAAC GAATCAAAGC ACAGGCAAAC AGGTCAATAA GGGAAGCACA
    AATGTACTGT CCAAGTACTC TGCCTTACAG GAGAAGGAAG CCCAGAATCT AGAAGTTGTA
    GCATTCTGTG AAGACATTTC AAAACTGAGG TCAAAGTTCC CACACGATGA CCTGTCTACC
    AACCCTGGCT ATGTTCTCAG CCCTGTGATC ACGCAGAGGG ATGCAGGAGG GGACAATGGG
    GGCAGTGTGA AAGTGTCCAT TGAGATTTCT GATTCTCAAC AGCCTGTAAC GTTTACTTGC
    GATGTGACTT CTCCAGTGGA CTTGCTCATA ATGCAGGCTT TGTGTTGGGT TCATGATGAC
    CTGAACCAGG TGGACATCAA TAGCTACGTT CTCAAGGTCT GCGGACAGGA GGAGGTCCTT
    CAGAATAAAC ACAGTCTAGG CAGTCATGAG TACGTGCAGA ACTGCAGGAA GTGGGAATCA
    GAGATCAAAC TACAGCTCCT GTTTCTTAGC ACAATGAGAA GAGATCTGGC CCGAACTGCA
    GAAGATGACA TCTCGCCCAT TGATTTGGAG AAATACCTTG GTCTAGTGGA GAGGCCATTT
    AAAGAGGCAG TCACAAGAGA AGGCCTTGCT GAATATCTAG AAGGCTTTCA TAAGCAAGTC
    AATCTCTGCC TTCAGAATGA GAACAACCAA TATAAAACAG TGGACAATGT GGTTCAGTCT
    GTTAAGAATC TGTGCTGTGC TCTGGATGAA GTTGAGACAC CAGCCATCAC TGATGCCGTC
    AAGAGGCTCA AGCGCTCTGT CAACCTTCCT AGAACACGCT CACCAGAGCA CTATGCTGAA
    TTGCAGGCTG GTGCCACATC TTCCGGAGGT TCAGCTAATG GTTACTCTAG CCCTGTAGAG
    GAGAGTTTAG CAGTGCTCAC AGATGCTGTC TATGAACTCG CCAAGCTCTA CCTTCAGTCT
    TTCTGCCCGA TTAGCCCTGG TTTCATGATA GAAGAGCACA AAGAAGATGA GAGGGACAGC
    AAAGAGGCCT CGGGCACCAC TGAACACTTA CAGTTCACAC TGTTTGCCGT TCATGGCATT
    CCTGCTACCT GGGTCAGCAG TTTTGAAAAA TACTACCTGA TGTGTGCCCT GACCCATAAC
    AACAGGAATC TCTTCAAACC TGTCCAGTCC AAAAAAGTGG GAACATACAA GAGTTTCTTC
    TACCACATCA AATGGGATGA ATTAATAAAC TTCCCTATTT CAGTGTCGCT GTTACCACTG
    GAGGCTATGC TTAGTCTTTC TCTCTATGGG GTCCTCAGTC AGAATGCAAA CAACTCACCA
    GATTCCAACA AGCAACGGAA AGGTCCAGAA CTTTTGGGAA AAGTTTCCAT GCCTCTGTTT
    GACTTCAGGA GGGTTCTGTC CAGAGGCAGT AAACTGCTGA GTTTGTGGAC TTCTCCTCAA
    GCCCTTCAGC CTGGAGCTGC AGGCAAAGGG AGGAATCCTA CAGAAAAGAT CATACTGCAG
    GTTGACTTCC CCAGTCCAGC GGTGGACGTG CTCTATGTTG GTCCTCAGGA GAACGGCTGC
    CCCAATCCTC AGTCTCTAGA CCCACTTGAT CAAGACGATC GAAGTGAGAT AGAAAAGCTG
    TGCGCACGAG CATCTACTTT CGGACTGTCA CGGGCGGACA GACAGCTGCT TTGGGACCAG
    AGGTACTATT GTCGGGAATA TGAATACAGT TTGCCGAAAA TCCTGGCCAG CGCTCCCAGC
    TGGGACTGGG GCAGTATGGG GGAAATCCAC GCACTTCTGC ACCACTGGCC TGCTCTGTCA
    CCCGTTTCAG CTCTTGAGCT CCTTGAATCC AAGTTTGCCG ACACAGAGGT GAGGAAAGTG
    GCTGTGAGCT GGATCCAGAG CAGCAGTGAT GATGAGCTGG CTGATTATCT CCCCCAGCTA
    GTGCAGGCAG TGAAGTTCGA GTGTCACCTT AACAATGCCT TGGTGAAGTT TCTGCTGTCT
    CGAGCTCTGG GAAATGTCAA CATTGCACAT TATCTTTACT GGCTGCTGAG GGATGCAGTG
    CAGGACCCGG CGTTTGGTCA GCGTTATGAG CGGATCCTCT GTGTGCTGCT GTGTCTGTGT
    GGGACCGGAC TGAGGGCTGA GCTGGAGAAG CAGACCCGAC TTGTGCAGCT GCTTGGAGCT
    CTGGCTGAAA AGGTTCGGCA GGCGAGCAGC TCAACCCGAC AGGTGGTTCT CCTCGAGGGT
    CTAGAGAGGG TTCAGTCTTT CTTCCAGAAG AACAGCTGTC GACTCCCTAT TAGCCCCAGT
    CTGGTGGCAA AAGAGCTCAA TATCAAGGTC TGCTCCTTTT TCAATTCCAA CGCTGTACCT
    CTAAAGATCG CACTGGTTAA CGCAGATCCG CTGGGAGATG AGATTAACGT CATGTTTAAG
    GTTGGAGAAG ACCTGCGGCA GGACATGTTG GCACTACAGA TGATTCGGAT TATGGATCGG
    ATCTGGCTTC AGGAAGGACT CGACCTGCGT ATTGTTAACT TTAAATGCAT CTCTACTGGG
    AAAGATAAAG GCATGGTGGA GTTGGTACCA TCTTCTGAAA CGCTGAGAAA GATTCAGGTG
    GAGTATGGAG TGACGGGTTC CTTCAAGGAC AAACCTCTTG CTGAGTGGCT TCGCAAGTAT
    AACCCTGCTG AGGATGAGTA TGAGAAGGCG TCTGAGAATT TTATCTACAC CTGTGCTGGA
    TGCTGTGTGG CCACTTACAT CCTCGGCATC TGCGATCGTC ACAACGACAA CATCATGCTG
    CGCTCCACTG GCCACATGTT CCACATAGAC TTCGGGAAGT TCCTGGGTCA CGCACAAATG
    ATTGGGAGTT TTAAAAGGGA CCGAGCACCG TTTGTCTTAA CCTCTGACAT GGCTTATGTC
    ATCAATGGAG GCGAAAGACC CACTAGTCGC TTTCAGCTGT TCGTTGACCT TTGCTCGCAA
    GCCTACAACC TGATCCGCAA ACACTCCAAC CTCTTCCTCA ACCTGCTATC TCTGATGACA
    CAATCAGGTT TACCCGAGCT GACCGGAGTC CAAGATTTGA AGTATGTGTA TGATGCTCTT
    CAGCCTCAGA CTACAGATGC AGAGGCTACC ATTTTCTTCA CAAGGTTGAT TGAATCCAGT
    CTGGGCAGTG TGGCTACAAA GTTTAATTTC TTTATCCACA ATCTGGCTCA GTTGCGTTTC
    TCTGGCCTTC CCTCCAATGA TGAACCCATC CTGTCGTTTG CTCCTCGGAC ATACACTATG
    AAGCAAGACG GCAAGATTCG GGACGCCTCT GTGTTTTCAT TTCAAAAGAG ATATAACCCA
    GACAAGCACT ATACGTATGT CATTCGGATT CTAAGGGAAG GTCAGAGCGA GCCACAGTTT
    GTTTTCCGCA CATTTGATGA GTTTCAGGAA TTGCACAACA AATTGACCAT CCTTTTTCAG
    TTGTGGAAGC TGCCAGGGTT TCCCAGTAAG ATGGTGCTGG GACGCACACA CATCAAGGAT
    GTGGCATCCA AGAGAAAGGT TGAATTGAAT AGTTATGTGC ACAATCTGAT GAGGAGTTCA
    ACAGAGGTCG CCCAGTGTGA TCTGATCTAC ACCTTCTTCC ACCCTATCGC AAGAGATGAC
    AAGACTGAAG GGGTTGATAC CCTATCAAAA ACTCCAGACA TGCCTCCAGT GAGTCCCACC
    ACAGGCCGTG TGGAGGGTGA GGTGAAGCTG TCAGTGTCCT ACAGAAACAG CACACTTTTC
    ATCATGGTCA TGCACATTAA AGACCTGATG TCCAATGACG GAGCAGACCC AAACCCTTAT
    GTGAAAACAT ACCTTCTCCC AGACCCCCAC AAAACTTCCA AACGCAAAAC AAAGATTGCG
    AGGAAGACAA GGAACCCAAC ATTTAATGAA ATGCTGGTGT ATAGCGGTTA CAGTAAGGAA
    ACACTGAAGC AAAGGGAACT TCAACTCAGT GTGCTCAGTG CCGAGTCTCT GCGAGAGAAC
    TGCTATTTGG GAGGAATAAC CCTCTGCCTT AAAGACTTTG ACCTCAGCCG AGAGACAGTC
    AAGTGGTACA AACTCACCAC GGTGCCCTAC TTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23385
    Clone ID Related Accession (Same CDS sequence) XM_021467820.1 , XM_005159305.3 , XM_005159306.2 , XM_021467821.1
    Accession Version XM_021467821.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5076bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCCCACA TATCCAGTGG CAATGGGTTT AAGTTTGACA GGCCAGCCTC TCCGGGGGTG 
    GTCCGGCCAA AAGGAATTGT GGGGAAGGAA GAGGCTTTAC GCATGGAGGT AGAGGCCTTC
    GATAAAATTA AGCGCGAGAA AAGGCACACA CTCCCAGTCA CAGCCTCTTC TGTTAATCCC
    CTTCCCAATC CAACAGCGCA AACATCCAGC AGCCGGCCAG AGAAAGACCT TATTGTATTC
    TGCGAATCAG AAACACAGAA GAAGGAGCAA AAAGACAACT TTGAGGACAT CGATTTGGAG
    AACCTGACAA AGGAGGAGCT GGAAAAGCTG CTTCTGGATG ACAGTTTTGG TGCAAACAAA
    ATGACAAGAC CTTCCTCTTT GCTAGGTTGC AATCTCAGCG CATCATATCC TGGCGGACAT
    GCCTTCAACC CCTCTTCGTT CCACTGGACA CCCACTCCAA CGCATGCACA GACTCCCATC
    TTCCCCTCTG CTCCATTCGC CAAGCCTCCT TGCTCTTTCC AGAATGGCTT TAGTCCAGCA
    ATGTCTCCAT TTATTCCACC GACCCCCTTT CTCTCCTTTA CTCCCATCCA ACCACCTGCT
    GCTCTGGTCT ACTCGCAGCC TGCCGTCACC CCAGAGATGG CCAAGCTGTT TGACAAGATT
    GCCAGCACTT CGGAATACTT GAAGAACGGC AGGTCCTCTA GCATGGAAAC CGAATCGGCA
    AGTGTCAAGT CTCTGGAGCC CCTACCTCTG CCCTCAGAAC CTCCTAACAT CAGCCGATTT
    GAATGGCTGG ACTTGGACCC TCTCAACAAG CGCAAGGAGG TGGAGGTCGA GGAGACACCT
    GCTGTTTCAA GCTGTCCTTT TATTGAGGAG TCTGGGAAAG CTAAAGACCC GTGGGATGCG
    GTTCTTCAGG ATGAGCATGA AACTGTAGAT AATGGCAGTC CCACCGCAGA GGAAAAGAGC
    AAGGCAACTC AACCCAGAAG AGCATCCACA GGGGCGGCTG TAACAAAAAG CCAATCCCTC
    AGTATATCTG CAAACTCAAC GAATCAAAGC ACAGGCAAAC AGGTCAATAA GGGAAGCACA
    AATGTACTGT CCAAGTACTC TGCCTTACAG GAGAAGGAAG CCCAGAATCT AGAAGTTGTA
    GCATTCTGTG AAGACATTTC AAAACTGAGG TCAAAGTTCC CACACGATGA CCTGTCTACC
    AACCCTGGCT ATGTTCTCAG CCCTGTGATC ACGCAGAGGG ATGCAGGAGG GGACAATGGG
    GGCAGTGTGA AAGTGTCCAT TGAGATTTCT GATTCTCAAC AGCCTGTAAC GTTTACTTGC
    GATGTGACTT CTCCAGTGGA CTTGCTCATA ATGCAGGCTT TGTGTTGGGT TCATGATGAC
    CTGAACCAGG TGGACATCAA TAGCTACGTT CTCAAGGTCT GCGGACAGGA GGAGGTCCTT
    CAGAATAAAC ACAGTCTAGG CAGTCATGAG TACGTGCAGA ACTGCAGGAA GTGGGAATCA
    GAGATCAAAC TACAGCTCCT GTTTCTTAGC ACAATGAGAA GAGATCTGGC CCGAACTGCA
    GAAGATGACA TCTCGCCCAT TGATTTGGAG AAATACCTTG GTCTAGTGGA GAGGCCATTT
    AAAGAGGCAG TCACAAGAGA AGGCCTTGCT GAATATCTAG AAGGCTTTCA TAAGCAAGTC
    AATCTCTGCC TTCAGAATGA GAACAACCAA TATAAAACAG TGGACAATGT GGTTCAGTCT
    GTTAAGAATC TGTGCTGTGC TCTGGATGAA GTTGAGACAC CAGCCATCAC TGATGCCGTC
    AAGAGGCTCA AGCGCTCTGT CAACCTTCCT AGAACACGCT CACCAGAGCA CTATGCTGAA
    TTGCAGGCTG GTGCCACATC TTCCGGAGGT TCAGCTAATG GTTACTCTAG CCCTGTAGAG
    GAGAGTTTAG CAGTGCTCAC AGATGCTGTC TATGAACTCG CCAAGCTCTA CCTTCAGTCT
    TTCTGCCCGA TTAGCCCTGG TTTCATGATA GAAGAGCACA AAGAAGATGA GAGGGACAGC
    AAAGAGGCCT CGGGCACCAC TGAACACTTA CAGTTCACAC TGTTTGCCGT TCATGGCATT
    CCTGCTACCT GGGTCAGCAG TTTTGAAAAA TACTACCTGA TGTGTGCCCT GACCCATAAC
    AACAGGAATC TCTTCAAACC TGTCCAGTCC AAAAAAGTGG GAACATACAA GAGTTTCTTC
    TACCACATCA AATGGGATGA ATTAATAAAC TTCCCTATTT CAGTGTCGCT GTTACCACTG
    GAGGCTATGC TTAGTCTTTC TCTCTATGGG GTCCTCAGTC AGAATGCAAA CAACTCACCA
    GATTCCAACA AGCAACGGAA AGGTCCAGAA CTTTTGGGAA AAGTTTCCAT GCCTCTGTTT
    GACTTCAGGA GGGTTCTGTC CAGAGGCAGT AAACTGCTGA GTTTGTGGAC TTCTCCTCAA
    GCCCTTCAGC CTGGAGCTGC AGGCAAAGGG AGGAATCCTA CAGAAAAGAT CATACTGCAG
    GTTGACTTCC CCAGTCCAGC GGTGGACGTG CTCTATGTTG GTCCTCAGGA GAACGGCTGC
    CCCAATCCTC AGTCTCTAGA CCCACTTGAT CAAGACGATC GAAGTGAGAT AGAAAAGCTG
    TGCGCACGAG CATCTACTTT CGGACTGTCA CGGGCGGACA GACAGCTGCT TTGGGACCAG
    AGGTACTATT GTCGGGAATA TGAATACAGT TTGCCGAAAA TCCTGGCCAG CGCTCCCAGC
    TGGGACTGGG GCAGTATGGG GGAAATCCAC GCACTTCTGC ACCACTGGCC TGCTCTGTCA
    CCCGTTTCAG CTCTTGAGCT CCTTGAATCC AAGTTTGCCG ACACAGAGGT GAGGAAAGTG
    GCTGTGAGCT GGATCCAGAG CAGCAGTGAT GATGAGCTGG CTGATTATCT CCCCCAGCTA
    GTGCAGGCAG TGAAGTTCGA GTGTCACCTT AACAATGCCT TGGTGAAGTT TCTGCTGTCT
    CGAGCTCTGG GAAATGTCAA CATTGCACAT TATCTTTACT GGCTGCTGAG GGATGCAGTG
    CAGGACCCGG CGTTTGGTCA GCGTTATGAG CGGATCCTCT GTGTGCTGCT GTGTCTGTGT
    GGGACCGGAC TGAGGGCTGA GCTGGAGAAG CAGACCCGAC TTGTGCAGCT GCTTGGAGCT
    CTGGCTGAAA AGGTTCGGCA GGCGAGCAGC TCAACCCGAC AGGTGGTTCT CCTCGAGGGT
    CTAGAGAGGG TTCAGTCTTT CTTCCAGAAG AACAGCTGTC GACTCCCTAT TAGCCCCAGT
    CTGGTGGCAA AAGAGCTCAA TATCAAGGTC TGCTCCTTTT TCAATTCCAA CGCTGTACCT
    CTAAAGATCG CACTGGTTAA CGCAGATCCG CTGGGAGATG AGATTAACGT CATGTTTAAG
    GTTGGAGAAG ACCTGCGGCA GGACATGTTG GCACTACAGA TGATTCGGAT TATGGATCGG
    ATCTGGCTTC AGGAAGGACT CGACCTGCGT ATTGTTAACT TTAAATGCAT CTCTACTGGG
    AAAGATAAAG GCATGGTGGA GTTGGTACCA TCTTCTGAAA CGCTGAGAAA GATTCAGGTG
    GAGTATGGAG TGACGGGTTC CTTCAAGGAC AAACCTCTTG CTGAGTGGCT TCGCAAGTAT
    AACCCTGCTG AGGATGAGTA TGAGAAGGCG TCTGAGAATT TTATCTACAC CTGTGCTGGA
    TGCTGTGTGG CCACTTACAT CCTCGGCATC TGCGATCGTC ACAACGACAA CATCATGCTG
    CGCTCCACTG GCCACATGTT CCACATAGAC TTCGGGAAGT TCCTGGGTCA CGCACAAATG
    ATTGGGAGTT TTAAAAGGGA CCGAGCACCG TTTGTCTTAA CCTCTGACAT GGCTTATGTC
    ATCAATGGAG GCGAAAGACC CACTAGTCGC TTTCAGCTGT TCGTTGACCT TTGCTCGCAA
    GCCTACAACC TGATCCGCAA ACACTCCAAC CTCTTCCTCA ACCTGCTATC TCTGATGACA
    CAATCAGGTT TACCCGAGCT GACCGGAGTC CAAGATTTGA AGTATGTGTA TGATGCTCTT
    CAGCCTCAGA CTACAGATGC AGAGGCTACC ATTTTCTTCA CAAGGTTGAT TGAATCCAGT
    CTGGGCAGTG TGGCTACAAA GTTTAATTTC TTTATCCACA ATCTGGCTCA GTTGCGTTTC
    TCTGGCCTTC CCTCCAATGA TGAACCCATC CTGTCGTTTG CTCCTCGGAC ATACACTATG
    AAGCAAGACG GCAAGATTCG GGACGCCTCT GTGTTTTCAT TTCAAAAGAG ATATAACCCA
    GACAAGCACT ATACGTATGT CATTCGGATT CTAAGGGAAG GTCAGAGCGA GCCACAGTTT
    GTTTTCCGCA CATTTGATGA GTTTCAGGAA TTGCACAACA AATTGACCAT CCTTTTTCAG
    TTGTGGAAGC TGCCAGGGTT TCCCAGTAAG ATGGTGCTGG GACGCACACA CATCAAGGAT
    GTGGCATCCA AGAGAAAGGT TGAATTGAAT AGTTATGTGC ACAATCTGAT GAGGAGTTCA
    ACAGAGGTCG CCCAGTGTGA TCTGATCTAC ACCTTCTTCC ACCCTATCGC AAGAGATGAC
    AAGACTGAAG GGGTTGATAC CCTATCAAAA ACTCCAGACA TGCCTCCAGT GAGTCCCACC
    ACAGGCCGTG TGGAGGGTGA GGTGAAGCTG TCAGTGTCCT ACAGAAACAG CACACTTTTC
    ATCATGGTCA TGCACATTAA AGACCTGATG TCCAATGACG GAGCAGACCC AAACCCTTAT
    GTGAAAACAT ACCTTCTCCC AGACCCCCAC AAAACTTCCA AACGCAAAAC AAAGATTGCG
    AGGAAGACAA GGAACCCAAC ATTTAATGAA ATGCTGGTGT ATAGCGGTTA CAGTAAGGAA
    ACACTGAAGC AAAGGGAACT TCAACTCAGT GTGCTCAGTG CCGAGTCTCT GCGAGAGAAC
    TGCTATTTGG GAGGAATAAC CCTCTGCCTT AAAGACTTTG ACCTCAGCCG AGAGACAGTC
    AAGTGGTACA AACTCACCAC GGTGCCCTAC TTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323496.1
    CDS308..5383
    Translation

    Target ORF information:

    RefSeq Version XM_021467821.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha (pik3c2a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467821.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCCCACA TATCCAGTGG CAATGGGTTT AAGTTTGACA GGCCAGCCTC TCCGGGGGTG 
    GTCCGGCCAA AAGGAATTGT GGGGAAGGAA GAGGCTTTAC GCATGGAGGT AGAGGCCTTC
    GATAAAATTA AGCGCGAGAA AAGGCACACA CTCCCAGTCA CAGCCTCTTC TGTTAATCCC
    CTTCCCAATC CAACAGCGCA AACATCCAGC AGCCGGCCAG AGAAAGACCT TATTGTATTC
    TGCGAATCAG AAACACAGAA GAAGGAGCAA AAAGACAACT TTGAGGACAT CGATTTGGAG
    AACCTGACAA AGGAGGAGCT GGAAAAGCTG CTTCTGGATG ACAGTTTTGG TGCAAACAAA
    ATGACAAGAC CTTCCTCTTT GCTAGGTTGC AATCTCAGCG CATCATATCC TGGCGGACAT
    GCCTTCAACC CCTCTTCGTT CCACTGGACA CCCACTCCAA CGCATGCACA GACTCCCATC
    TTCCCCTCTG CTCCATTCGC CAAGCCTCCT TGCTCTTTCC AGAATGGCTT TAGTCCAGCA
    ATGTCTCCAT TTATTCCACC GACCCCCTTT CTCTCCTTTA CTCCCATCCA ACCACCTGCT
    GCTCTGGTCT ACTCGCAGCC TGCCGTCACC CCAGAGATGG CCAAGCTGTT TGACAAGATT
    GCCAGCACTT CGGAATACTT GAAGAACGGC AGGTCCTCTA GCATGGAAAC CGAATCGGCA
    AGTGTCAAGT CTCTGGAGCC CCTACCTCTG CCCTCAGAAC CTCCTAACAT CAGCCGATTT
    GAATGGCTGG ACTTGGACCC TCTCAACAAG CGCAAGGAGG TGGAGGTCGA GGAGACACCT
    GCTGTTTCAA GCTGTCCTTT TATTGAGGAG TCTGGGAAAG CTAAAGACCC GTGGGATGCG
    GTTCTTCAGG ATGAGCATGA AACTGTAGAT AATGGCAGTC CCACCGCAGA GGAAAAGAGC
    AAGGCAACTC AACCCAGAAG AGCATCCACA GGGGCGGCTG TAACAAAAAG CCAATCCCTC
    AGTATATCTG CAAACTCAAC GAATCAAAGC ACAGGCAAAC AGGTCAATAA GGGAAGCACA
    AATGTACTGT CCAAGTACTC TGCCTTACAG GAGAAGGAAG CCCAGAATCT AGAAGTTGTA
    GCATTCTGTG AAGACATTTC AAAACTGAGG TCAAAGTTCC CACACGATGA CCTGTCTACC
    AACCCTGGCT ATGTTCTCAG CCCTGTGATC ACGCAGAGGG ATGCAGGAGG GGACAATGGG
    GGCAGTGTGA AAGTGTCCAT TGAGATTTCT GATTCTCAAC AGCCTGTAAC GTTTACTTGC
    GATGTGACTT CTCCAGTGGA CTTGCTCATA ATGCAGGCTT TGTGTTGGGT TCATGATGAC
    CTGAACCAGG TGGACATCAA TAGCTACGTT CTCAAGGTCT GCGGACAGGA GGAGGTCCTT
    CAGAATAAAC ACAGTCTAGG CAGTCATGAG TACGTGCAGA ACTGCAGGAA GTGGGAATCA
    GAGATCAAAC TACAGCTCCT GTTTCTTAGC ACAATGAGAA GAGATCTGGC CCGAACTGCA
    GAAGATGACA TCTCGCCCAT TGATTTGGAG AAATACCTTG GTCTAGTGGA GAGGCCATTT
    AAAGAGGCAG TCACAAGAGA AGGCCTTGCT GAATATCTAG AAGGCTTTCA TAAGCAAGTC
    AATCTCTGCC TTCAGAATGA GAACAACCAA TATAAAACAG TGGACAATGT GGTTCAGTCT
    GTTAAGAATC TGTGCTGTGC TCTGGATGAA GTTGAGACAC CAGCCATCAC TGATGCCGTC
    AAGAGGCTCA AGCGCTCTGT CAACCTTCCT AGAACACGCT CACCAGAGCA CTATGCTGAA
    TTGCAGGCTG GTGCCACATC TTCCGGAGGT TCAGCTAATG GTTACTCTAG CCCTGTAGAG
    GAGAGTTTAG CAGTGCTCAC AGATGCTGTC TATGAACTCG CCAAGCTCTA CCTTCAGTCT
    TTCTGCCCGA TTAGCCCTGG TTTCATGATA GAAGAGCACA AAGAAGATGA GAGGGACAGC
    AAAGAGGCCT CGGGCACCAC TGAACACTTA CAGTTCACAC TGTTTGCCGT TCATGGCATT
    CCTGCTACCT GGGTCAGCAG TTTTGAAAAA TACTACCTGA TGTGTGCCCT GACCCATAAC
    AACAGGAATC TCTTCAAACC TGTCCAGTCC AAAAAAGTGG GAACATACAA GAGTTTCTTC
    TACCACATCA AATGGGATGA ATTAATAAAC TTCCCTATTT CAGTGTCGCT GTTACCACTG
    GAGGCTATGC TTAGTCTTTC TCTCTATGGG GTCCTCAGTC AGAATGCAAA CAACTCACCA
    GATTCCAACA AGCAACGGAA AGGTCCAGAA CTTTTGGGAA AAGTTTCCAT GCCTCTGTTT
    GACTTCAGGA GGGTTCTGTC CAGAGGCAGT AAACTGCTGA GTTTGTGGAC TTCTCCTCAA
    GCCCTTCAGC CTGGAGCTGC AGGCAAAGGG AGGAATCCTA CAGAAAAGAT CATACTGCAG
    GTTGACTTCC CCAGTCCAGC GGTGGACGTG CTCTATGTTG GTCCTCAGGA GAACGGCTGC
    CCCAATCCTC AGTCTCTAGA CCCACTTGAT CAAGACGATC GAAGTGAGAT AGAAAAGCTG
    TGCGCACGAG CATCTACTTT CGGACTGTCA CGGGCGGACA GACAGCTGCT TTGGGACCAG
    AGGTACTATT GTCGGGAATA TGAATACAGT TTGCCGAAAA TCCTGGCCAG CGCTCCCAGC
    TGGGACTGGG GCAGTATGGG GGAAATCCAC GCACTTCTGC ACCACTGGCC TGCTCTGTCA
    CCCGTTTCAG CTCTTGAGCT CCTTGAATCC AAGTTTGCCG ACACAGAGGT GAGGAAAGTG
    GCTGTGAGCT GGATCCAGAG CAGCAGTGAT GATGAGCTGG CTGATTATCT CCCCCAGCTA
    GTGCAGGCAG TGAAGTTCGA GTGTCACCTT AACAATGCCT TGGTGAAGTT TCTGCTGTCT
    CGAGCTCTGG GAAATGTCAA CATTGCACAT TATCTTTACT GGCTGCTGAG GGATGCAGTG
    CAGGACCCGG CGTTTGGTCA GCGTTATGAG CGGATCCTCT GTGTGCTGCT GTGTCTGTGT
    GGGACCGGAC TGAGGGCTGA GCTGGAGAAG CAGACCCGAC TTGTGCAGCT GCTTGGAGCT
    CTGGCTGAAA AGGTTCGGCA GGCGAGCAGC TCAACCCGAC AGGTGGTTCT CCTCGAGGGT
    CTAGAGAGGG TTCAGTCTTT CTTCCAGAAG AACAGCTGTC GACTCCCTAT TAGCCCCAGT
    CTGGTGGCAA AAGAGCTCAA TATCAAGGTC TGCTCCTTTT TCAATTCCAA CGCTGTACCT
    CTAAAGATCG CACTGGTTAA CGCAGATCCG CTGGGAGATG AGATTAACGT CATGTTTAAG
    GTTGGAGAAG ACCTGCGGCA GGACATGTTG GCACTACAGA TGATTCGGAT TATGGATCGG
    ATCTGGCTTC AGGAAGGACT CGACCTGCGT ATTGTTAACT TTAAATGCAT CTCTACTGGG
    AAAGATAAAG GCATGGTGGA GTTGGTACCA TCTTCTGAAA CGCTGAGAAA GATTCAGGTG
    GAGTATGGAG TGACGGGTTC CTTCAAGGAC AAACCTCTTG CTGAGTGGCT TCGCAAGTAT
    AACCCTGCTG AGGATGAGTA TGAGAAGGCG TCTGAGAATT TTATCTACAC CTGTGCTGGA
    TGCTGTGTGG CCACTTACAT CCTCGGCATC TGCGATCGTC ACAACGACAA CATCATGCTG
    CGCTCCACTG GCCACATGTT CCACATAGAC TTCGGGAAGT TCCTGGGTCA CGCACAAATG
    ATTGGGAGTT TTAAAAGGGA CCGAGCACCG TTTGTCTTAA CCTCTGACAT GGCTTATGTC
    ATCAATGGAG GCGAAAGACC CACTAGTCGC TTTCAGCTGT TCGTTGACCT TTGCTCGCAA
    GCCTACAACC TGATCCGCAA ACACTCCAAC CTCTTCCTCA ACCTGCTATC TCTGATGACA
    CAATCAGGTT TACCCGAGCT GACCGGAGTC CAAGATTTGA AGTATGTGTA TGATGCTCTT
    CAGCCTCAGA CTACAGATGC AGAGGCTACC ATTTTCTTCA CAAGGTTGAT TGAATCCAGT
    CTGGGCAGTG TGGCTACAAA GTTTAATTTC TTTATCCACA ATCTGGCTCA GTTGCGTTTC
    TCTGGCCTTC CCTCCAATGA TGAACCCATC CTGTCGTTTG CTCCTCGGAC ATACACTATG
    AAGCAAGACG GCAAGATTCG GGACGCCTCT GTGTTTTCAT TTCAAAAGAG ATATAACCCA
    GACAAGCACT ATACGTATGT CATTCGGATT CTAAGGGAAG GTCAGAGCGA GCCACAGTTT
    GTTTTCCGCA CATTTGATGA GTTTCAGGAA TTGCACAACA AATTGACCAT CCTTTTTCAG
    TTGTGGAAGC TGCCAGGGTT TCCCAGTAAG ATGGTGCTGG GACGCACACA CATCAAGGAT
    GTGGCATCCA AGAGAAAGGT TGAATTGAAT AGTTATGTGC ACAATCTGAT GAGGAGTTCA
    ACAGAGGTCG CCCAGTGTGA TCTGATCTAC ACCTTCTTCC ACCCTATCGC AAGAGATGAC
    AAGACTGAAG GGGTTGATAC CCTATCAAAA ACTCCAGACA TGCCTCCAGT GAGTCCCACC
    ACAGGCCGTG TGGAGGGTGA GGTGAAGCTG TCAGTGTCCT ACAGAAACAG CACACTTTTC
    ATCATGGTCA TGCACATTAA AGACCTGATG TCCAATGACG GAGCAGACCC AAACCCTTAT
    GTGAAAACAT ACCTTCTCCC AGACCCCCAC AAAACTTCCA AACGCAAAAC AAAGATTGCG
    AGGAAGACAA GGAACCCAAC ATTTAATGAA ATGCTGGTGT ATAGCGGTTA CAGTAAGGAA
    ACACTGAAGC AAAGGGAACT TCAACTCAGT GTGCTCAGTG CCGAGTCTCT GCGAGAGAAC
    TGCTATTTGG GAGGAATAAC CCTCTGCCTT AAAGACTTTG ACCTCAGCCG AGAGACAGTC
    AAGTGGTACA AACTCACCAC GGTGCCCTAC TTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23386
    Clone ID Related Accession (Same CDS sequence) XM_694937.7 , XM_021467822.1
    Accession Version XM_694937.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_694937.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCCCACA TATCCAGTGG CAATGGGTTT AAGTTTGACA GGCCAGCCTC TCCGGGGGTG 
    GTCCGGCCAA AAGGAATTGT GGGGAAGGAA GAGGCTTTAC GCATGGAGGT AGAGGCCTTC
    GATAAAATTA AGCGCGAGAA AAGGCACACA CTCCCAGTCA CAGCCTCTTC TGTTAATCCC
    CTTCCCAATC CAACAGCGCA AACATCCAGC AGCCGGCCAG AGAAAGACCT TATTGTATTC
    TGCGAATCAG AAACACAGAA GAAGGAGCAA AAAGACAACT TTGAGGACAT CGATTTGGAG
    AACCTGACAA AGGAGGAGCT GGAAAAGCTG CTTCTGGATG ACAGTTTTGG TGCAAACAAA
    ATGACAAGAC CTTCCTCTTT GCTAGGTTGC AATCTCAGCG CATCATATCC TGGCGGACAT
    GCCTTCAACC CCTCTTCGTT CCACTGGACA CCCACTCCAA CGCATGCACA GACTCCCATC
    TTCCCCTCTG CTCCATTCGC CAAGCCTCCT TGCTCTTTCC AGAATGGCTT TAGTCCAGCA
    ATGTCTCCAT TTATTCCACC GACCCCCTTT CTCTCCTTTA CTCCCATCCA ACCACCTGCT
    GCTCTGGTCT ACTCGCAGCC TGCCGTCACC CCAGAGATGG CCAAGCTGTT TGACAAGATT
    GCCAGCACTT CGGAATACTT GAAGAACGGC AGGTCCTCTA GCATGGAAAC CGAATCGGCA
    AGTGTCAAGT CTCTGGAGCC CCTACCTCTG CCCTCAGAAC CTCCTAACAT CAGCCGATTT
    GAATGGCTGG ACTTGGACCC TCTCAACAAG CGCAAGGAGG TGGAGGTCGA GGAGACACCT
    GCTGTTTCAA GCTGTCCTTT TATTGAGGAG TCTGGGAAAG CTAAAGACCC GTGGGATGCG
    GTTCTTCAGG ATGAGCATGA AACTGTAGAT AATGGCAGTC CCACCGCAGA GGAAAAGAGC
    AAGGCAACTC AACCCAGAAG AGCATCCACA GGGGCGGCTG TAACAAAAAG CCAATCCCTC
    AGTATATCTG CAAACTCAAC GAATCAAAGC ACAGGCAAAC AGGTCAATAA GGGAAGCACA
    AATGTACTGT CCAAGTACTC TGCCTTACAG GAGAAGGAAG CCCAGAATCT AGAAGTTGTA
    GCATTCTGTG AAGACATTTC AAAACTGAGG TCAAAGTTCC CACACGATGA CCTGTCTACC
    AACCCTGGCT ATGTTCTCAG CCCTGTGATC ACGCAGAGGG ATGCAGGAGG GGACAATGGG
    GGCAGTGTGA AAGTGTCCAT TGAGATTTCT GATTCTCAAC AGCCTGTAAC GTTTACTTGC
    GATGTGACTT CTCCAGTGGA CTTGCTCATA ATGCAGGCTT TGTGTTGGGT TCATGATGAC
    CTGAACCAGG TGGACATCAA TAGCTACGTT CTCAAGGTCT GCGGACAGGA GGAGGTCCTT
    CAGAATAAAC ACAGTCTAGG CAGTCATGAG TACGTGCAGA ACTGCAGGAA GTGGGAATCA
    GAGATCAAAC TACAGCTCCT GTTTCTTAGC ACAATGAGAA GAGATCTGGC CCGAACTGCA
    GAAGATGACA TCTCGCCCAT TGATTTGGAG AAATACCTTG GTCTAGTGGA GAGGCCATTT
    AAAGAGGCAG TCACAAGAGA AGGCCTTGCT GAATATCTAG AAGGCTTTCA TAAGCAAGTC
    AATCTCTGCC TTCAGAATGA GAACAACCAA TATAAAACAG TGGACAATGT GGTTCAGTCT
    GTTAAGAATC TGTGCTGTGC TCTGGATGAA GTTGAGACAC CAGCCATCAC TGATGCCGTC
    AAGAGGCTCA AGCGCTCTGT CAACCTTCCT AGAACACGCT CACCAGAGGC TGGTGCCACA
    TCTTCCGGAG GTTCAGCTAA TGGTTACTCT AGCCCTGTAG AGGAGAGTTT AGCAGTGCTC
    ACAGATGCTG TCTATGAACT CGCCAAGCTC TACCTTCAGT CTTTCTGCCC GATTAGCCCT
    GGTTTCATGA TAGAAGAGCA CAAAGAAGAT GAGAGGGACA GCAAAGAGGC CTCGGGCACC
    ACTGAACACT TACAGTTCAC ACTGTTTGCC GTTCATGGCA TTCCTGCTAC CTGGGTCAGC
    AGTTTTGAAA AATACTACCT GATGTGTGCC CTGACCCATA ACAACAGGAA TCTCTTCAAA
    CCTGTCCAGT CCAAAAAAGT GGGAACATAC AAGAGTTTCT TCTACCACAT CAAATGGGAT
    GAATTAATAA ACTTCCCTAT TTCAGTGTCG CTGTTACCAC TGGAGGCTAT GCTTAGTCTT
    TCTCTCTATG GGGTCCTCAG TCAGAATGCA AACAACTCAC CAGATTCCAA CAAGCAACGG
    AAAGGTCCAG AACTTTTGGG AAAAGTTTCC ATGCCTCTGT TTGACTTCAG GAGGGTTCTG
    TCCAGAGGCA GTAAACTGCT GAGTTTGTGG ACTTCTCCTC AAGCCCTTCA GCCTGGAGCT
    GCAGGCAAAG GGAGGAATCC TACAGAAAAG ATCATACTGC AGGTTGACTT CCCCAGTCCA
    GCGGTGGACG TGCTCTATGT TGGTCCTCAG GAGAACGGCT GCCCCAATCC TCAGTCTCTA
    GACCCACTTG ATCAAGACGA TCGAAGTGAG ATAGAAAAGC TGTGCGCACG AGCATCTACT
    TTCGGACTGT CACGGGCGGA CAGACAGCTG CTTTGGGACC AGAGGTACTA TTGTCGGGAA
    TATGAATACA GTTTGCCGAA AATCCTGGCC AGCGCTCCCA GCTGGGACTG GGGCAGTATG
    GGGGAAATCC ACGCACTTCT GCACCACTGG CCTGCTCTGT CACCCGTTTC AGCTCTTGAG
    CTCCTTGAAT CCAAGTTTGC CGACACAGAG GTGAGGAAAG TGGCTGTGAG CTGGATCCAG
    AGCAGCAGTG ATGATGAGCT GGCTGATTAT CTCCCCCAGC TAGTGCAGGC AGTGAAGTTC
    GAGTGTCACC TTAACAATGC CTTGGTGAAG TTTCTGCTGT CTCGAGCTCT GGGAAATGTC
    AACATTGCAC ATTATCTTTA CTGGCTGCTG AGGGATGCAG TGCAGGACCC GGCGTTTGGT
    CAGCGTTATG AGCGGATCCT CTGTGTGCTG CTGTGTCTGT GTGGGACCGG ACTGAGGGCT
    GAGCTGGAGA AGCAGACCCG ACTTGTGCAG CTGCTTGGAG CTCTGGCTGA AAAGGTTCGG
    CAGGCGAGCA GCTCAACCCG ACAGGTGGTT CTCCTCGAGG GTCTAGAGAG GGTTCAGTCT
    TTCTTCCAGA AGAACAGCTG TCGACTCCCT ATTAGCCCCA GTCTGGTGGC AAAAGAGCTC
    AATATCAAGG TCTGCTCCTT TTTCAATTCC AACGCTGTAC CTCTAAAGAT CGCACTGGTT
    AACGCAGATC CGCTGGGAGA TGAGATTAAC GTCATGTTTA AGGTTGGAGA AGACCTGCGG
    CAGGACATGT TGGCACTACA GATGATTCGG ATTATGGATC GGATCTGGCT TCAGGAAGGA
    CTCGACCTGC GTATTGTTAA CTTTAAATGC ATCTCTACTG GGAAAGATAA AGGCATGGTG
    GAGTTGGTAC CATCTTCTGA AACGCTGAGA AAGATTCAGG TGGAGTATGG AGTGACGGGT
    TCCTTCAAGG ACAAACCTCT TGCTGAGTGG CTTCGCAAGT ATAACCCTGC TGAGGATGAG
    TATGAGAAGG CGTCTGAGAA TTTTATCTAC ACCTGTGCTG GATGCTGTGT GGCCACTTAC
    ATCCTCGGCA TCTGCGATCG TCACAACGAC AACATCATGC TGCGCTCCAC TGGCCACATG
    TTCCACATAG ACTTCGGGAA GTTCCTGGGT CACGCACAAA TGATTGGGAG TTTTAAAAGG
    GACCGAGCAC CGTTTGTCTT AACCTCTGAC ATGGCTTATG TCATCAATGG AGGCGAAAGA
    CCCACTAGTC GCTTTCAGCT GTTCGTTGAC CTTTGCTCGC AAGCCTACAA CCTGATCCGC
    AAACACTCCA ACCTCTTCCT CAACCTGCTA TCTCTGATGA CACAATCAGG TTTACCCGAG
    CTGACCGGAG TCCAAGATTT GAAGTATGTG TATGATGCTC TTCAGCCTCA GACTACAGAT
    GCAGAGGCTA CCATTTTCTT CACAAGGTTG ATTGAATCCA GTCTGGGCAG TGTGGCTACA
    AAGTTTAATT TCTTTATCCA CAATCTGGCT CAGTTGCGTT TCTCTGGCCT TCCCTCCAAT
    GATGAACCCA TCCTGTCGTT TGCTCCTCGG ACATACACTA TGAAGCAAGA CGGCAAGATT
    CGGGACGCCT CTGTGTTTTC ATTTCAAAAG AGATATAACC CAGACAAGCA CTATACGTAT
    GTCATTCGGA TTCTAAGGGA AGGTCAGAGC GAGCCACAGT TTGTTTTCCG CACATTTGAT
    GAGTTTCAGG AATTGCACAA CAAATTGACC ATCCTTTTTC AGTTGTGGAA GCTGCCAGGG
    TTTCCCAGTA AGATGGTGCT GGGACGCACA CACATCAAGG ATGTGGCATC CAAGAGAAAG
    GTTGAATTGA ATAGTTATGT GCACAATCTG ATGAGGAGTT CAACAGAGGT CGCCCAGTGT
    GATCTGATCT ACACCTTCTT CCACCCTATC GCAAGAGATG ACAAGACTGA AGGGGTTGAT
    ACCCTATCAA AAACTCCAGA CATGCCTCCA GTGAGTCCCA CCACAGGCCG TGTGGAGGGT
    GAGGTGAAGC TGTCAGTGTC CTACAGAAAC AGCACACTTT TCATCATGGT CATGCACATT
    AAAGACCTGA TGTCCAATGA CGGAGCAGAC CCAAACCCTT ATGTGAAAAC ATACCTTCTC
    CCAGACCCCC ACAAAACTTC CAAACGCAAA ACAAAGATTG CGAGGAAGAC AAGGAACCCA
    ACATTTAATG AAATGCTGGT GTATAGCGGT TACAGTAAGG AAACACTGAA GCAAAGGGAA
    CTTCAACTCA GTGTGCTCAG TGCCGAGTCT CTGCGAGAGA ACTGCTATTT GGGAGGAATA
    ACCCTCTGCC TTAAAGACTT TGACCTCAGC CGAGAGACAG TCAAGTGGTA CAAACTCACC
    ACGGTGCCCT ACTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_700029.4
    CDS159..5216
    Translation

    Target ORF information:

    RefSeq Version XM_694937.7
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha (pik3c2a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_694937.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCCCACA TATCCAGTGG CAATGGGTTT AAGTTTGACA GGCCAGCCTC TCCGGGGGTG 
    GTCCGGCCAA AAGGAATTGT GGGGAAGGAA GAGGCTTTAC GCATGGAGGT AGAGGCCTTC
    GATAAAATTA AGCGCGAGAA AAGGCACACA CTCCCAGTCA CAGCCTCTTC TGTTAATCCC
    CTTCCCAATC CAACAGCGCA AACATCCAGC AGCCGGCCAG AGAAAGACCT TATTGTATTC
    TGCGAATCAG AAACACAGAA GAAGGAGCAA AAAGACAACT TTGAGGACAT CGATTTGGAG
    AACCTGACAA AGGAGGAGCT GGAAAAGCTG CTTCTGGATG ACAGTTTTGG TGCAAACAAA
    ATGACAAGAC CTTCCTCTTT GCTAGGTTGC AATCTCAGCG CATCATATCC TGGCGGACAT
    GCCTTCAACC CCTCTTCGTT CCACTGGACA CCCACTCCAA CGCATGCACA GACTCCCATC
    TTCCCCTCTG CTCCATTCGC CAAGCCTCCT TGCTCTTTCC AGAATGGCTT TAGTCCAGCA
    ATGTCTCCAT TTATTCCACC GACCCCCTTT CTCTCCTTTA CTCCCATCCA ACCACCTGCT
    GCTCTGGTCT ACTCGCAGCC TGCCGTCACC CCAGAGATGG CCAAGCTGTT TGACAAGATT
    GCCAGCACTT CGGAATACTT GAAGAACGGC AGGTCCTCTA GCATGGAAAC CGAATCGGCA
    AGTGTCAAGT CTCTGGAGCC CCTACCTCTG CCCTCAGAAC CTCCTAACAT CAGCCGATTT
    GAATGGCTGG ACTTGGACCC TCTCAACAAG CGCAAGGAGG TGGAGGTCGA GGAGACACCT
    GCTGTTTCAA GCTGTCCTTT TATTGAGGAG TCTGGGAAAG CTAAAGACCC GTGGGATGCG
    GTTCTTCAGG ATGAGCATGA AACTGTAGAT AATGGCAGTC CCACCGCAGA GGAAAAGAGC
    AAGGCAACTC AACCCAGAAG AGCATCCACA GGGGCGGCTG TAACAAAAAG CCAATCCCTC
    AGTATATCTG CAAACTCAAC GAATCAAAGC ACAGGCAAAC AGGTCAATAA GGGAAGCACA
    AATGTACTGT CCAAGTACTC TGCCTTACAG GAGAAGGAAG CCCAGAATCT AGAAGTTGTA
    GCATTCTGTG AAGACATTTC AAAACTGAGG TCAAAGTTCC CACACGATGA CCTGTCTACC
    AACCCTGGCT ATGTTCTCAG CCCTGTGATC ACGCAGAGGG ATGCAGGAGG GGACAATGGG
    GGCAGTGTGA AAGTGTCCAT TGAGATTTCT GATTCTCAAC AGCCTGTAAC GTTTACTTGC
    GATGTGACTT CTCCAGTGGA CTTGCTCATA ATGCAGGCTT TGTGTTGGGT TCATGATGAC
    CTGAACCAGG TGGACATCAA TAGCTACGTT CTCAAGGTCT GCGGACAGGA GGAGGTCCTT
    CAGAATAAAC ACAGTCTAGG CAGTCATGAG TACGTGCAGA ACTGCAGGAA GTGGGAATCA
    GAGATCAAAC TACAGCTCCT GTTTCTTAGC ACAATGAGAA GAGATCTGGC CCGAACTGCA
    GAAGATGACA TCTCGCCCAT TGATTTGGAG AAATACCTTG GTCTAGTGGA GAGGCCATTT
    AAAGAGGCAG TCACAAGAGA AGGCCTTGCT GAATATCTAG AAGGCTTTCA TAAGCAAGTC
    AATCTCTGCC TTCAGAATGA GAACAACCAA TATAAAACAG TGGACAATGT GGTTCAGTCT
    GTTAAGAATC TGTGCTGTGC TCTGGATGAA GTTGAGACAC CAGCCATCAC TGATGCCGTC
    AAGAGGCTCA AGCGCTCTGT CAACCTTCCT AGAACACGCT CACCAGAGGC TGGTGCCACA
    TCTTCCGGAG GTTCAGCTAA TGGTTACTCT AGCCCTGTAG AGGAGAGTTT AGCAGTGCTC
    ACAGATGCTG TCTATGAACT CGCCAAGCTC TACCTTCAGT CTTTCTGCCC GATTAGCCCT
    GGTTTCATGA TAGAAGAGCA CAAAGAAGAT GAGAGGGACA GCAAAGAGGC CTCGGGCACC
    ACTGAACACT TACAGTTCAC ACTGTTTGCC GTTCATGGCA TTCCTGCTAC CTGGGTCAGC
    AGTTTTGAAA AATACTACCT GATGTGTGCC CTGACCCATA ACAACAGGAA TCTCTTCAAA
    CCTGTCCAGT CCAAAAAAGT GGGAACATAC AAGAGTTTCT TCTACCACAT CAAATGGGAT
    GAATTAATAA ACTTCCCTAT TTCAGTGTCG CTGTTACCAC TGGAGGCTAT GCTTAGTCTT
    TCTCTCTATG GGGTCCTCAG TCAGAATGCA AACAACTCAC CAGATTCCAA CAAGCAACGG
    AAAGGTCCAG AACTTTTGGG AAAAGTTTCC ATGCCTCTGT TTGACTTCAG GAGGGTTCTG
    TCCAGAGGCA GTAAACTGCT GAGTTTGTGG ACTTCTCCTC AAGCCCTTCA GCCTGGAGCT
    GCAGGCAAAG GGAGGAATCC TACAGAAAAG ATCATACTGC AGGTTGACTT CCCCAGTCCA
    GCGGTGGACG TGCTCTATGT TGGTCCTCAG GAGAACGGCT GCCCCAATCC TCAGTCTCTA
    GACCCACTTG ATCAAGACGA TCGAAGTGAG ATAGAAAAGC TGTGCGCACG AGCATCTACT
    TTCGGACTGT CACGGGCGGA CAGACAGCTG CTTTGGGACC AGAGGTACTA TTGTCGGGAA
    TATGAATACA GTTTGCCGAA AATCCTGGCC AGCGCTCCCA GCTGGGACTG GGGCAGTATG
    GGGGAAATCC ACGCACTTCT GCACCACTGG CCTGCTCTGT CACCCGTTTC AGCTCTTGAG
    CTCCTTGAAT CCAAGTTTGC CGACACAGAG GTGAGGAAAG TGGCTGTGAG CTGGATCCAG
    AGCAGCAGTG ATGATGAGCT GGCTGATTAT CTCCCCCAGC TAGTGCAGGC AGTGAAGTTC
    GAGTGTCACC TTAACAATGC CTTGGTGAAG TTTCTGCTGT CTCGAGCTCT GGGAAATGTC
    AACATTGCAC ATTATCTTTA CTGGCTGCTG AGGGATGCAG TGCAGGACCC GGCGTTTGGT
    CAGCGTTATG AGCGGATCCT CTGTGTGCTG CTGTGTCTGT GTGGGACCGG ACTGAGGGCT
    GAGCTGGAGA AGCAGACCCG ACTTGTGCAG CTGCTTGGAG CTCTGGCTGA AAAGGTTCGG
    CAGGCGAGCA GCTCAACCCG ACAGGTGGTT CTCCTCGAGG GTCTAGAGAG GGTTCAGTCT
    TTCTTCCAGA AGAACAGCTG TCGACTCCCT ATTAGCCCCA GTCTGGTGGC AAAAGAGCTC
    AATATCAAGG TCTGCTCCTT TTTCAATTCC AACGCTGTAC CTCTAAAGAT CGCACTGGTT
    AACGCAGATC CGCTGGGAGA TGAGATTAAC GTCATGTTTA AGGTTGGAGA AGACCTGCGG
    CAGGACATGT TGGCACTACA GATGATTCGG ATTATGGATC GGATCTGGCT TCAGGAAGGA
    CTCGACCTGC GTATTGTTAA CTTTAAATGC ATCTCTACTG GGAAAGATAA AGGCATGGTG
    GAGTTGGTAC CATCTTCTGA AACGCTGAGA AAGATTCAGG TGGAGTATGG AGTGACGGGT
    TCCTTCAAGG ACAAACCTCT TGCTGAGTGG CTTCGCAAGT ATAACCCTGC TGAGGATGAG
    TATGAGAAGG CGTCTGAGAA TTTTATCTAC ACCTGTGCTG GATGCTGTGT GGCCACTTAC
    ATCCTCGGCA TCTGCGATCG TCACAACGAC AACATCATGC TGCGCTCCAC TGGCCACATG
    TTCCACATAG ACTTCGGGAA GTTCCTGGGT CACGCACAAA TGATTGGGAG TTTTAAAAGG
    GACCGAGCAC CGTTTGTCTT AACCTCTGAC ATGGCTTATG TCATCAATGG AGGCGAAAGA
    CCCACTAGTC GCTTTCAGCT GTTCGTTGAC CTTTGCTCGC AAGCCTACAA CCTGATCCGC
    AAACACTCCA ACCTCTTCCT CAACCTGCTA TCTCTGATGA CACAATCAGG TTTACCCGAG
    CTGACCGGAG TCCAAGATTT GAAGTATGTG TATGATGCTC TTCAGCCTCA GACTACAGAT
    GCAGAGGCTA CCATTTTCTT CACAAGGTTG ATTGAATCCA GTCTGGGCAG TGTGGCTACA
    AAGTTTAATT TCTTTATCCA CAATCTGGCT CAGTTGCGTT TCTCTGGCCT TCCCTCCAAT
    GATGAACCCA TCCTGTCGTT TGCTCCTCGG ACATACACTA TGAAGCAAGA CGGCAAGATT
    CGGGACGCCT CTGTGTTTTC ATTTCAAAAG AGATATAACC CAGACAAGCA CTATACGTAT
    GTCATTCGGA TTCTAAGGGA AGGTCAGAGC GAGCCACAGT TTGTTTTCCG CACATTTGAT
    GAGTTTCAGG AATTGCACAA CAAATTGACC ATCCTTTTTC AGTTGTGGAA GCTGCCAGGG
    TTTCCCAGTA AGATGGTGCT GGGACGCACA CACATCAAGG ATGTGGCATC CAAGAGAAAG
    GTTGAATTGA ATAGTTATGT GCACAATCTG ATGAGGAGTT CAACAGAGGT CGCCCAGTGT
    GATCTGATCT ACACCTTCTT CCACCCTATC GCAAGAGATG ACAAGACTGA AGGGGTTGAT
    ACCCTATCAA AAACTCCAGA CATGCCTCCA GTGAGTCCCA CCACAGGCCG TGTGGAGGGT
    GAGGTGAAGC TGTCAGTGTC CTACAGAAAC AGCACACTTT TCATCATGGT CATGCACATT
    AAAGACCTGA TGTCCAATGA CGGAGCAGAC CCAAACCCTT ATGTGAAAAC ATACCTTCTC
    CCAGACCCCC ACAAAACTTC CAAACGCAAA ACAAAGATTG CGAGGAAGAC AAGGAACCCA
    ACATTTAATG AAATGCTGGT GTATAGCGGT TACAGTAAGG AAACACTGAA GCAAAGGGAA
    CTTCAACTCA GTGTGCTCAG TGCCGAGTCT CTGCGAGAGA ACTGCTATTT GGGAGGAATA
    ACCCTCTGCC TTAAAGACTT TGACCTCAGC CGAGAGACAG TCAAGTGGTA CAAACTCACC
    ACGGTGCCCT ACTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa23386
    Clone ID Related Accession (Same CDS sequence) XM_694937.7 , XM_021467822.1
    Accession Version XM_021467822.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5058bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product phosphatidylinositol 4-phosphate 3-kinase C2 domain-containing subunit alpha isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCCCACA TATCCAGTGG CAATGGGTTT AAGTTTGACA GGCCAGCCTC TCCGGGGGTG 
    GTCCGGCCAA AAGGAATTGT GGGGAAGGAA GAGGCTTTAC GCATGGAGGT AGAGGCCTTC
    GATAAAATTA AGCGCGAGAA AAGGCACACA CTCCCAGTCA CAGCCTCTTC TGTTAATCCC
    CTTCCCAATC CAACAGCGCA AACATCCAGC AGCCGGCCAG AGAAAGACCT TATTGTATTC
    TGCGAATCAG AAACACAGAA GAAGGAGCAA AAAGACAACT TTGAGGACAT CGATTTGGAG
    AACCTGACAA AGGAGGAGCT GGAAAAGCTG CTTCTGGATG ACAGTTTTGG TGCAAACAAA
    ATGACAAGAC CTTCCTCTTT GCTAGGTTGC AATCTCAGCG CATCATATCC TGGCGGACAT
    GCCTTCAACC CCTCTTCGTT CCACTGGACA CCCACTCCAA CGCATGCACA GACTCCCATC
    TTCCCCTCTG CTCCATTCGC CAAGCCTCCT TGCTCTTTCC AGAATGGCTT TAGTCCAGCA
    ATGTCTCCAT TTATTCCACC GACCCCCTTT CTCTCCTTTA CTCCCATCCA ACCACCTGCT
    GCTCTGGTCT ACTCGCAGCC TGCCGTCACC CCAGAGATGG CCAAGCTGTT TGACAAGATT
    GCCAGCACTT CGGAATACTT GAAGAACGGC AGGTCCTCTA GCATGGAAAC CGAATCGGCA
    AGTGTCAAGT CTCTGGAGCC CCTACCTCTG CCCTCAGAAC CTCCTAACAT CAGCCGATTT
    GAATGGCTGG ACTTGGACCC TCTCAACAAG CGCAAGGAGG TGGAGGTCGA GGAGACACCT
    GCTGTTTCAA GCTGTCCTTT TATTGAGGAG TCTGGGAAAG CTAAAGACCC GTGGGATGCG
    GTTCTTCAGG ATGAGCATGA AACTGTAGAT AATGGCAGTC CCACCGCAGA GGAAAAGAGC
    AAGGCAACTC AACCCAGAAG AGCATCCACA GGGGCGGCTG TAACAAAAAG CCAATCCCTC
    AGTATATCTG CAAACTCAAC GAATCAAAGC ACAGGCAAAC AGGTCAATAA GGGAAGCACA
    AATGTACTGT CCAAGTACTC TGCCTTACAG GAGAAGGAAG CCCAGAATCT AGAAGTTGTA
    GCATTCTGTG AAGACATTTC AAAACTGAGG TCAAAGTTCC CACACGATGA CCTGTCTACC
    AACCCTGGCT ATGTTCTCAG CCCTGTGATC ACGCAGAGGG ATGCAGGAGG GGACAATGGG
    GGCAGTGTGA AAGTGTCCAT TGAGATTTCT GATTCTCAAC AGCCTGTAAC GTTTACTTGC
    GATGTGACTT CTCCAGTGGA CTTGCTCATA ATGCAGGCTT TGTGTTGGGT TCATGATGAC
    CTGAACCAGG TGGACATCAA TAGCTACGTT CTCAAGGTCT GCGGACAGGA GGAGGTCCTT
    CAGAATAAAC ACAGTCTAGG CAGTCATGAG TACGTGCAGA ACTGCAGGAA GTGGGAATCA
    GAGATCAAAC TACAGCTCCT GTTTCTTAGC ACAATGAGAA GAGATCTGGC CCGAACTGCA
    GAAGATGACA TCTCGCCCAT TGATTTGGAG AAATACCTTG GTCTAGTGGA GAGGCCATTT
    AAAGAGGCAG TCACAAGAGA AGGCCTTGCT GAATATCTAG AAGGCTTTCA TAAGCAAGTC
    AATCTCTGCC TTCAGAATGA GAACAACCAA TATAAAACAG TGGACAATGT GGTTCAGTCT
    GTTAAGAATC TGTGCTGTGC TCTGGATGAA GTTGAGACAC CAGCCATCAC TGATGCCGTC
    AAGAGGCTCA AGCGCTCTGT CAACCTTCCT AGAACACGCT CACCAGAGGC TGGTGCCACA
    TCTTCCGGAG GTTCAGCTAA TGGTTACTCT AGCCCTGTAG AGGAGAGTTT AGCAGTGCTC
    ACAGATGCTG TCTATGAACT CGCCAAGCTC TACCTTCAGT CTTTCTGCCC GATTAGCCCT
    GGTTTCATGA TAGAAGAGCA CAAAGAAGAT GAGAGGGACA GCAAAGAGGC CTCGGGCACC
    ACTGAACACT TACAGTTCAC ACTGTTTGCC GTTCATGGCA TTCCTGCTAC CTGGGTCAGC
    AGTTTTGAAA AATACTACCT GATGTGTGCC CTGACCCATA ACAACAGGAA TCTCTTCAAA
    CCTGTCCAGT CCAAAAAAGT GGGAACATAC AAGAGTTTCT TCTACCACAT CAAATGGGAT
    GAATTAATAA ACTTCCCTAT TTCAGTGTCG CTGTTACCAC TGGAGGCTAT GCTTAGTCTT
    TCTCTCTATG GGGTCCTCAG TCAGAATGCA AACAACTCAC CAGATTCCAA CAAGCAACGG
    AAAGGTCCAG AACTTTTGGG AAAAGTTTCC ATGCCTCTGT TTGACTTCAG GAGGGTTCTG
    TCCAGAGGCA GTAAACTGCT GAGTTTGTGG ACTTCTCCTC AAGCCCTTCA GCCTGGAGCT
    GCAGGCAAAG GGAGGAATCC TACAGAAAAG ATCATACTGC AGGTTGACTT CCCCAGTCCA
    GCGGTGGACG TGCTCTATGT TGGTCCTCAG GAGAACGGCT GCCCCAATCC TCAGTCTCTA
    GACCCACTTG ATCAAGACGA TCGAAGTGAG ATAGAAAAGC TGTGCGCACG AGCATCTACT
    TTCGGACTGT CACGGGCGGA CAGACAGCTG CTTTGGGACC AGAGGTACTA TTGTCGGGAA
    TATGAATACA GTTTGCCGAA AATCCTGGCC AGCGCTCCCA GCTGGGACTG GGGCAGTATG
    GGGGAAATCC ACGCACTTCT GCACCACTGG CCTGCTCTGT CACCCGTTTC AGCTCTTGAG
    CTCCTTGAAT CCAAGTTTGC CGACACAGAG GTGAGGAAAG TGGCTGTGAG CTGGATCCAG
    AGCAGCAGTG ATGATGAGCT GGCTGATTAT CTCCCCCAGC TAGTGCAGGC AGTGAAGTTC
    GAGTGTCACC TTAACAATGC CTTGGTGAAG TTTCTGCTGT CTCGAGCTCT GGGAAATGTC
    AACATTGCAC ATTATCTTTA CTGGCTGCTG AGGGATGCAG TGCAGGACCC GGCGTTTGGT
    CAGCGTTATG AGCGGATCCT CTGTGTGCTG CTGTGTCTGT GTGGGACCGG ACTGAGGGCT
    GAGCTGGAGA AGCAGACCCG ACTTGTGCAG CTGCTTGGAG CTCTGGCTGA AAAGGTTCGG
    CAGGCGAGCA GCTCAACCCG ACAGGTGGTT CTCCTCGAGG GTCTAGAGAG GGTTCAGTCT
    TTCTTCCAGA AGAACAGCTG TCGACTCCCT ATTAGCCCCA GTCTGGTGGC AAAAGAGCTC
    AATATCAAGG TCTGCTCCTT TTTCAATTCC AACGCTGTAC CTCTAAAGAT CGCACTGGTT
    AACGCAGATC CGCTGGGAGA TGAGATTAAC GTCATGTTTA AGGTTGGAGA AGACCTGCGG
    CAGGACATGT TGGCACTACA GATGATTCGG ATTATGGATC GGATCTGGCT TCAGGAAGGA
    CTCGACCTGC GTATTGTTAA CTTTAAATGC ATCTCTACTG GGAAAGATAA AGGCATGGTG
    GAGTTGGTAC CATCTTCTGA AACGCTGAGA AAGATTCAGG TGGAGTATGG AGTGACGGGT
    TCCTTCAAGG ACAAACCTCT TGCTGAGTGG CTTCGCAAGT ATAACCCTGC TGAGGATGAG
    TATGAGAAGG CGTCTGAGAA TTTTATCTAC ACCTGTGCTG GATGCTGTGT GGCCACTTAC
    ATCCTCGGCA TCTGCGATCG TCACAACGAC AACATCATGC TGCGCTCCAC TGGCCACATG
    TTCCACATAG ACTTCGGGAA GTTCCTGGGT CACGCACAAA TGATTGGGAG TTTTAAAAGG
    GACCGAGCAC CGTTTGTCTT AACCTCTGAC ATGGCTTATG TCATCAATGG AGGCGAAAGA
    CCCACTAGTC GCTTTCAGCT GTTCGTTGAC CTTTGCTCGC AAGCCTACAA CCTGATCCGC
    AAACACTCCA ACCTCTTCCT CAACCTGCTA TCTCTGATGA CACAATCAGG TTTACCCGAG
    CTGACCGGAG TCCAAGATTT GAAGTATGTG TATGATGCTC TTCAGCCTCA GACTACAGAT
    GCAGAGGCTA CCATTTTCTT CACAAGGTTG ATTGAATCCA GTCTGGGCAG TGTGGCTACA
    AAGTTTAATT TCTTTATCCA CAATCTGGCT CAGTTGCGTT TCTCTGGCCT TCCCTCCAAT
    GATGAACCCA TCCTGTCGTT TGCTCCTCGG ACATACACTA TGAAGCAAGA CGGCAAGATT
    CGGGACGCCT CTGTGTTTTC ATTTCAAAAG AGATATAACC CAGACAAGCA CTATACGTAT
    GTCATTCGGA TTCTAAGGGA AGGTCAGAGC GAGCCACAGT TTGTTTTCCG CACATTTGAT
    GAGTTTCAGG AATTGCACAA CAAATTGACC ATCCTTTTTC AGTTGTGGAA GCTGCCAGGG
    TTTCCCAGTA AGATGGTGCT GGGACGCACA CACATCAAGG ATGTGGCATC CAAGAGAAAG
    GTTGAATTGA ATAGTTATGT GCACAATCTG ATGAGGAGTT CAACAGAGGT CGCCCAGTGT
    GATCTGATCT ACACCTTCTT CCACCCTATC GCAAGAGATG ACAAGACTGA AGGGGTTGAT
    ACCCTATCAA AAACTCCAGA CATGCCTCCA GTGAGTCCCA CCACAGGCCG TGTGGAGGGT
    GAGGTGAAGC TGTCAGTGTC CTACAGAAAC AGCACACTTT TCATCATGGT CATGCACATT
    AAAGACCTGA TGTCCAATGA CGGAGCAGAC CCAAACCCTT ATGTGAAAAC ATACCTTCTC
    CCAGACCCCC ACAAAACTTC CAAACGCAAA ACAAAGATTG CGAGGAAGAC AAGGAACCCA
    ACATTTAATG AAATGCTGGT GTATAGCGGT TACAGTAAGG AAACACTGAA GCAAAGGGAA
    CTTCAACTCA GTGTGCTCAG TGCCGAGTCT CTGCGAGAGA ACTGCTATTT GGGAGGAATA
    ACCCTCTGCC TTAAAGACTT TGACCTCAGC CGAGAGACAG TCAAGTGGTA CAAACTCACC
    ACGGTGCCCT ACTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323497.1
    CDS177..5234
    Translation

    Target ORF information:

    RefSeq Version XM_021467822.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phosphatidylinositol-4-phosphate 3-kinase, catalytic subunit type 2 alpha (pik3c2a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467822.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCCCACA TATCCAGTGG CAATGGGTTT AAGTTTGACA GGCCAGCCTC TCCGGGGGTG 
    GTCCGGCCAA AAGGAATTGT GGGGAAGGAA GAGGCTTTAC GCATGGAGGT AGAGGCCTTC
    GATAAAATTA AGCGCGAGAA AAGGCACACA CTCCCAGTCA CAGCCTCTTC TGTTAATCCC
    CTTCCCAATC CAACAGCGCA AACATCCAGC AGCCGGCCAG AGAAAGACCT TATTGTATTC
    TGCGAATCAG AAACACAGAA GAAGGAGCAA AAAGACAACT TTGAGGACAT CGATTTGGAG
    AACCTGACAA AGGAGGAGCT GGAAAAGCTG CTTCTGGATG ACAGTTTTGG TGCAAACAAA
    ATGACAAGAC CTTCCTCTTT GCTAGGTTGC AATCTCAGCG CATCATATCC TGGCGGACAT
    GCCTTCAACC CCTCTTCGTT CCACTGGACA CCCACTCCAA CGCATGCACA GACTCCCATC
    TTCCCCTCTG CTCCATTCGC CAAGCCTCCT TGCTCTTTCC AGAATGGCTT TAGTCCAGCA
    ATGTCTCCAT TTATTCCACC GACCCCCTTT CTCTCCTTTA CTCCCATCCA ACCACCTGCT
    GCTCTGGTCT ACTCGCAGCC TGCCGTCACC CCAGAGATGG CCAAGCTGTT TGACAAGATT
    GCCAGCACTT CGGAATACTT GAAGAACGGC AGGTCCTCTA GCATGGAAAC CGAATCGGCA
    AGTGTCAAGT CTCTGGAGCC CCTACCTCTG CCCTCAGAAC CTCCTAACAT CAGCCGATTT
    GAATGGCTGG ACTTGGACCC TCTCAACAAG CGCAAGGAGG TGGAGGTCGA GGAGACACCT
    GCTGTTTCAA GCTGTCCTTT TATTGAGGAG TCTGGGAAAG CTAAAGACCC GTGGGATGCG
    GTTCTTCAGG ATGAGCATGA AACTGTAGAT AATGGCAGTC CCACCGCAGA GGAAAAGAGC
    AAGGCAACTC AACCCAGAAG AGCATCCACA GGGGCGGCTG TAACAAAAAG CCAATCCCTC
    AGTATATCTG CAAACTCAAC GAATCAAAGC ACAGGCAAAC AGGTCAATAA GGGAAGCACA
    AATGTACTGT CCAAGTACTC TGCCTTACAG GAGAAGGAAG CCCAGAATCT AGAAGTTGTA
    GCATTCTGTG AAGACATTTC AAAACTGAGG TCAAAGTTCC CACACGATGA CCTGTCTACC
    AACCCTGGCT ATGTTCTCAG CCCTGTGATC ACGCAGAGGG ATGCAGGAGG GGACAATGGG
    GGCAGTGTGA AAGTGTCCAT TGAGATTTCT GATTCTCAAC AGCCTGTAAC GTTTACTTGC
    GATGTGACTT CTCCAGTGGA CTTGCTCATA ATGCAGGCTT TGTGTTGGGT TCATGATGAC
    CTGAACCAGG TGGACATCAA TAGCTACGTT CTCAAGGTCT GCGGACAGGA GGAGGTCCTT
    CAGAATAAAC ACAGTCTAGG CAGTCATGAG TACGTGCAGA ACTGCAGGAA GTGGGAATCA
    GAGATCAAAC TACAGCTCCT GTTTCTTAGC ACAATGAGAA GAGATCTGGC CCGAACTGCA
    GAAGATGACA TCTCGCCCAT TGATTTGGAG AAATACCTTG GTCTAGTGGA GAGGCCATTT
    AAAGAGGCAG TCACAAGAGA AGGCCTTGCT GAATATCTAG AAGGCTTTCA TAAGCAAGTC
    AATCTCTGCC TTCAGAATGA GAACAACCAA TATAAAACAG TGGACAATGT GGTTCAGTCT
    GTTAAGAATC TGTGCTGTGC TCTGGATGAA GTTGAGACAC CAGCCATCAC TGATGCCGTC
    AAGAGGCTCA AGCGCTCTGT CAACCTTCCT AGAACACGCT CACCAGAGGC TGGTGCCACA
    TCTTCCGGAG GTTCAGCTAA TGGTTACTCT AGCCCTGTAG AGGAGAGTTT AGCAGTGCTC
    ACAGATGCTG TCTATGAACT CGCCAAGCTC TACCTTCAGT CTTTCTGCCC GATTAGCCCT
    GGTTTCATGA TAGAAGAGCA CAAAGAAGAT GAGAGGGACA GCAAAGAGGC CTCGGGCACC
    ACTGAACACT TACAGTTCAC ACTGTTTGCC GTTCATGGCA TTCCTGCTAC CTGGGTCAGC
    AGTTTTGAAA AATACTACCT GATGTGTGCC CTGACCCATA ACAACAGGAA TCTCTTCAAA
    CCTGTCCAGT CCAAAAAAGT GGGAACATAC AAGAGTTTCT TCTACCACAT CAAATGGGAT
    GAATTAATAA ACTTCCCTAT TTCAGTGTCG CTGTTACCAC TGGAGGCTAT GCTTAGTCTT
    TCTCTCTATG GGGTCCTCAG TCAGAATGCA AACAACTCAC CAGATTCCAA CAAGCAACGG
    AAAGGTCCAG AACTTTTGGG AAAAGTTTCC ATGCCTCTGT TTGACTTCAG GAGGGTTCTG
    TCCAGAGGCA GTAAACTGCT GAGTTTGTGG ACTTCTCCTC AAGCCCTTCA GCCTGGAGCT
    GCAGGCAAAG GGAGGAATCC TACAGAAAAG ATCATACTGC AGGTTGACTT CCCCAGTCCA
    GCGGTGGACG TGCTCTATGT TGGTCCTCAG GAGAACGGCT GCCCCAATCC TCAGTCTCTA
    GACCCACTTG ATCAAGACGA TCGAAGTGAG ATAGAAAAGC TGTGCGCACG AGCATCTACT
    TTCGGACTGT CACGGGCGGA CAGACAGCTG CTTTGGGACC AGAGGTACTA TTGTCGGGAA
    TATGAATACA GTTTGCCGAA AATCCTGGCC AGCGCTCCCA GCTGGGACTG GGGCAGTATG
    GGGGAAATCC ACGCACTTCT GCACCACTGG CCTGCTCTGT CACCCGTTTC AGCTCTTGAG
    CTCCTTGAAT CCAAGTTTGC CGACACAGAG GTGAGGAAAG TGGCTGTGAG CTGGATCCAG
    AGCAGCAGTG ATGATGAGCT GGCTGATTAT CTCCCCCAGC TAGTGCAGGC AGTGAAGTTC
    GAGTGTCACC TTAACAATGC CTTGGTGAAG TTTCTGCTGT CTCGAGCTCT GGGAAATGTC
    AACATTGCAC ATTATCTTTA CTGGCTGCTG AGGGATGCAG TGCAGGACCC GGCGTTTGGT
    CAGCGTTATG AGCGGATCCT CTGTGTGCTG CTGTGTCTGT GTGGGACCGG ACTGAGGGCT
    GAGCTGGAGA AGCAGACCCG ACTTGTGCAG CTGCTTGGAG CTCTGGCTGA AAAGGTTCGG
    CAGGCGAGCA GCTCAACCCG ACAGGTGGTT CTCCTCGAGG GTCTAGAGAG GGTTCAGTCT
    TTCTTCCAGA AGAACAGCTG TCGACTCCCT ATTAGCCCCA GTCTGGTGGC AAAAGAGCTC
    AATATCAAGG TCTGCTCCTT TTTCAATTCC AACGCTGTAC CTCTAAAGAT CGCACTGGTT
    AACGCAGATC CGCTGGGAGA TGAGATTAAC GTCATGTTTA AGGTTGGAGA AGACCTGCGG
    CAGGACATGT TGGCACTACA GATGATTCGG ATTATGGATC GGATCTGGCT TCAGGAAGGA
    CTCGACCTGC GTATTGTTAA CTTTAAATGC ATCTCTACTG GGAAAGATAA AGGCATGGTG
    GAGTTGGTAC CATCTTCTGA AACGCTGAGA AAGATTCAGG TGGAGTATGG AGTGACGGGT
    TCCTTCAAGG ACAAACCTCT TGCTGAGTGG CTTCGCAAGT ATAACCCTGC TGAGGATGAG
    TATGAGAAGG CGTCTGAGAA TTTTATCTAC ACCTGTGCTG GATGCTGTGT GGCCACTTAC
    ATCCTCGGCA TCTGCGATCG TCACAACGAC AACATCATGC TGCGCTCCAC TGGCCACATG
    TTCCACATAG ACTTCGGGAA GTTCCTGGGT CACGCACAAA TGATTGGGAG TTTTAAAAGG
    GACCGAGCAC CGTTTGTCTT AACCTCTGAC ATGGCTTATG TCATCAATGG AGGCGAAAGA
    CCCACTAGTC GCTTTCAGCT GTTCGTTGAC CTTTGCTCGC AAGCCTACAA CCTGATCCGC
    AAACACTCCA ACCTCTTCCT CAACCTGCTA TCTCTGATGA CACAATCAGG TTTACCCGAG
    CTGACCGGAG TCCAAGATTT GAAGTATGTG TATGATGCTC TTCAGCCTCA GACTACAGAT
    GCAGAGGCTA CCATTTTCTT CACAAGGTTG ATTGAATCCA GTCTGGGCAG TGTGGCTACA
    AAGTTTAATT TCTTTATCCA CAATCTGGCT CAGTTGCGTT TCTCTGGCCT TCCCTCCAAT
    GATGAACCCA TCCTGTCGTT TGCTCCTCGG ACATACACTA TGAAGCAAGA CGGCAAGATT
    CGGGACGCCT CTGTGTTTTC ATTTCAAAAG AGATATAACC CAGACAAGCA CTATACGTAT
    GTCATTCGGA TTCTAAGGGA AGGTCAGAGC GAGCCACAGT TTGTTTTCCG CACATTTGAT
    GAGTTTCAGG AATTGCACAA CAAATTGACC ATCCTTTTTC AGTTGTGGAA GCTGCCAGGG
    TTTCCCAGTA AGATGGTGCT GGGACGCACA CACATCAAGG ATGTGGCATC CAAGAGAAAG
    GTTGAATTGA ATAGTTATGT GCACAATCTG ATGAGGAGTT CAACAGAGGT CGCCCAGTGT
    GATCTGATCT ACACCTTCTT CCACCCTATC GCAAGAGATG ACAAGACTGA AGGGGTTGAT
    ACCCTATCAA AAACTCCAGA CATGCCTCCA GTGAGTCCCA CCACAGGCCG TGTGGAGGGT
    GAGGTGAAGC TGTCAGTGTC CTACAGAAAC AGCACACTTT TCATCATGGT CATGCACATT
    AAAGACCTGA TGTCCAATGA CGGAGCAGAC CCAAACCCTT ATGTGAAAAC ATACCTTCTC
    CCAGACCCCC ACAAAACTTC CAAACGCAAA ACAAAGATTG CGAGGAAGAC AAGGAACCCA
    ACATTTAATG AAATGCTGGT GTATAGCGGT TACAGTAAGG AAACACTGAA GCAAAGGGAA
    CTTCAACTCA GTGTGCTCAG TGCCGAGTCT CTGCGAGAGA ACTGCTATTT GGGAGGAATA
    ACCCTCTGCC TTAAAGACTT TGACCTCAGC CGAGAGACAG TCAAGTGGTA CAAACTCACC
    ACGGTGCCCT ACTTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.