tmc2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tmc2a
Entrez Gene ID	571240
Full Name	transmembrane channel-like 2a
Synonyms	si:dkey-267i17.6
General protein information	Preferred Names transmembrane channel-like 2a Names transmembrane channel-like protein 2-A transmembrane channel-like protein 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001302237.1	NP_001289166.1	transmembrane channel-like protein 2-A

Pathways from BioSystems

Homologies

	TMC2	XP_001110485.2
Bos taurus (cattle)	TMC2	NP_001107238.1
Gallus gallus (chicken)	TMC2	NP_001034413.1
Canis lupus familiaris (dog)	TMC2	XP_005635398.1
Rattus norvegicus (Norway rat)	Tmc2	NP_001099980.1
Danio rerio (zebrafish)	si:dkey-267i17.6	XP_699903.5
Xenopus tropicalis (tropical clawed frog)	LOC100490320	XP_002938353.2
Homo sapiens (human)	TMC2	NP_542789.2
Pan troglodytes (chimpanzee)	TMC2	XP_001156297.1
Mus musculus (house mouse)	Tmc2	NP_619596.1

Gene Ontology
Bibliography

Related articles in PubMed

A molecular basis for water motion detection by the mechanosensory lateral line of zebrafish.
Chou SW, Chen Z, Zhu S, Davis RW, Hu J, Liu L, Fernando CA, Kindig K, Brown WC, Stepanyan R, McDermott BM Jr
Nature communications8(1)2234(2017 Dec)

Integration of Tmc1/2 into the mechanotransduction complex in zebrafish hair cells is regulated by Transmembrane O-methyltransferase (Tomt).
Erickson T, Morgan CP, Olt J, Hardy K, Busch-Nentwich E, Maeda R, Clemens R, Krey JF, Nechiporuk A, Barr-Gillespie PG, Marcotti W, Nicolson T
eLife6(2017 May)

Identification of sensory hair-cell transcripts by thiouracil-tagging in zebrafish.
Erickson T, Nicolson T
BMC genomics16842(2015 Oct)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results identify an evolutionarily conserved association between the fish and mouse orthologs of PCDH15 and TMC1 and TMC2, supporting the notion that TMCs are key components of the transduction complex in hair cells.
Title: Tip-link protein protocadherin 15 interacts with transmembrane channel-like proteins TMC1 and TMC2.

The following tmc2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tmc2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa46097	NM_001302237.1 Latest version!	Danio rerio transmembrane channel-like 2a (tmc2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tmc2a ( NM_001302237.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001289166.1 Danio rerio transmembrane channel-like 2a (tmc2a), mRNA.

Price & Availability↑

CloneID	ODa46097
Clone ID Related Accession (Same CDS sequence)	NM_001302237.1
Accession Version	NM_001302237.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-13
Organism	Danio rerio(zebrafish)
Product	transmembrane channel-like protein 2-A
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from KM115407.1. On Oct 7, 2014 this sequence version replaced XM_009301834.1. ##Evidence-Data-START## Transcript exon combination :: KM115407.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCCAAAGA AAAGTGATAC AACAAGAAAA CTGGAAGATG TGGGAATAGA AATTGATGGG 
GATGTGGACA GTGCTGAAGA GGACAAGAAA TCCAAAGGCA AGGGTGGTAA AAAAGCAGCA 
GGCGGTAAGC GAGGTAAAGC CAGTGAGGAT GGTGAGGATG AAGATGAGGA TGATAAACCC 
CCCAAAGGTC GTCGGGCAGC CAATAAAAAG AAACCAGCAC CAGTAGATGA GGAAGACAGT 
GATGATGATA TTCCACAAAG GAGATCTGCT GGGAACAGGA GACGAGGAAA TGTGCGAGAG 
AGAGGAGATG GAGACAAAAA GAAGTCTGGC AAAAAAGGCA GGAGAGGTGG AAAAAAGAAT 
GAAAAAGGGA AAGGAAAAGA CAGTGACAAA GACAGTGACA AAGATGAAAA GAAGAAAAAG 
AACAGTAGTG GTGATGAATC CGATTCAGAT GAAGAGGATG AGTCCATGTC TGAAGGAGAA 
ATGGCCAAAC TCATGGAGGA GGTGGAGGAA AAGAAGAAAT TAATAGCAAA CATCAGGAAC 
AAGCCCTGGC GAATGAGAAG ACGACTTAAA GTCCTCAAGG AGGCTCAGCA GTTTGTGGAT 
AAATTTGAAG GTGCTTTGGG CAAAGGGAAA GGCAGAAAGC TGTATGCCTA CAAAGTTATG 
ATGATGAAGA AATGGATAAA GTTTAAAAGG GATTTTGAAA ATTTTAGAAC GGCTTGCATC 
CCATGGGAGC GGAAAATAAA GGAAGTTGAG AGTCACTTTG GCTCCTCTGT TGCTTCATAC 
TTTATTTTCC TCCGTTGGAT GTATGGCATG AACTTGGTCC TCTTCAGCTT AACTTTTGGC 
CTGGTTGTGA TACCAGAGGT TCTCATGGGA CTTCCATATG GGTCCATTCC TCGGAAAACT 
GTGCCGAGAG AAGACCAAGA TACTGCAATG GACTATTCTG TTCTTACGGA TTTCAATGGT 
TACTGCAAGT ACTCAGTACT TTTCTATGGC TACTATAATA ACCAGAGAAC CATTGGCTTC 
CTGAAGTTTC GTCTCCCTCT GTCCTACCTC ATGGTGGGCA TTGGGACCTT CGGGTATAGC 
CTCATGGTTG TGATCAGAAC GATGGCTAAA AACGCAGATG TGGGGGGAGG GGACGGAGAA 
GACAATGAGT TCACCTTTGC CTGGAAGATG TTCACCAGCT GGGATTATCT TATTGGGAAC 
GCTGAGACAG CTGACAATAA ATACGCCTCC ATCACCACCA GCTTCAAAGA ATCCATTGTG 
GATGAGCAGG AGAACCAAAA GGATGAAAAC ATTCATCTGA GAAGATTCTT ACGTGTCCTA 
GCCAACTTTC TCATCACCTG TACATTGGGT GGAAGTGGCT ATCTCATCTA TTTTGTAGTC 
AAACGCTCTC AAGAGTTTCA AAACATGGAC AACCTCTCAT GGTATGAGAA GAATGAGCTT 
GAGATCATCA TGTCTCTGTT GGGACTGGTG GGTCCAATGC TCTTTGAGAC CATCGCAGAG 
TTGGAGGAGT ACCATCCACG TATCGCTCTC AAGTGGCAAC TTGGACGTAT ATTTGCACTC 
TTTCTTGGCA ATCTCTACAC ATTTTTGCTG GCTCTGTTTG ATGAGGTCAA TGCAAAACTT 
GAAGAAGAGG AGTCTATAAA AAATGCCAGC ATTTGGTTCT TGAAGGAATA CTATGCCAAC 
TACACAGCCA ACAACCCAAA TGACACAGGC ACTCCACCTC CTATCAACCC AGCGGATGCC 
ATCAGAGGCC CATGCTGGGA AACCACAGTG GGAGTAGAGT TTGTGAAGCT GACTATATCA 
GATATACAGG TGACATATCT GACCATCCTC ATCGGTGATT TCTTGAGAGC GTTCATTGTA 
CGTTTCCTGA ACTATTGCTG GTGCTGGGAT TTGGAAGCCG GATGGCCTTC CTATGGTGAA 
TTTGACATAA GCGGCAATGT GCTTGGTTTG GTATTTAACC AAGGAATGAT CTGGATGGGG 
GCATTCTATG CTCCCGGTCT TGTAGGCATC AATGTTCTGC GGCTCCTTTC ATCTATGTAC 
TATCAATGCT GGGCTGTAAT GGCCTGTAAT GTCCCACATG AGAGGGTCTT TAAAGCTTCT 
AAATCCAATA ACTTCTACAT GGGCCTGTTA TTGCTCATCC TCTTTCTGAG TCTCCTACCT 
GTGGTTTACA CCATTATGTC TTTGCCACCA TCTTTTGATT GTGGACCTTT CAGTGGAAAG 
GAAAGAATGT TCGACGTTGT TATGGAGACC ATTGATCTGG ATCTACCAGC ATTTATGGGG 
ACTCTCTTTG GATATGTCGC AAACCCGGGA CTCGTTATAT CTGCAGTACT TCTCATGGTA 
CTGGCCATTT ACTACCTGAA TTCTGTGTCA GAGGCCTACA AGAACTCCAA CAATGAGCTA 
AAAAAGAAAA TGCAGATGGC AAGAGATGAG GAGAAGAACA GACGGAACAA CAAAGACAGC 
ACTAACCAAG TGATGAAAGA CCTAGAGGAT CTGCTTCCCA ATCGCCCTCC AACGCCTCCT 
TCTCCTCGAG AGAATATCGC AGAAAAAAAC CAGGGCCAGG GTGGAAAATC TGCAAAAGTT 
AAACCAGGCA CAGCTGGCGG TGTGCATCTG CAGAAAGATG TGTCACTCGC ATCAGCCAAT 
CCTAATGCAC GGGGGCCTGT GACACGAGCG CCAGGCCCCA GGGGTCCTGG ACCTCTACCT 
GGCCAACCAG GGGCAGGTAG GGGTCAAGGT CCACCCCCTA GACGGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001289166.1
CDS	72..2822
Translation	MPKKSDTTRKLEDVGIEIDGDVDSAEEDKKSKGKGGKKAAGGKRGKASEDGEDEDEDDKPPKGRRAANKKKPAPVDEEDSDDDIPQRRSAGNRRRGNVRERGDGDKKKSGKKGRRGGKKNEKGKGKDSDKDSDKDEKKKKNSSGDESDSDEEDESMSEGEMAKLMEEVEEKKKLIANIRNKPWRMRRRLKVLKEAQQFVDKFEGALGKGKGRKLYAYKVMMMKKWIKFKRDFENFRTACIPWERKIKEVESHFGSSVASYFIFLRWMYGMNLVLFSLTFGLVVIPEVLMGLPYGSIPRKTVPREDQDTAMDYSVLTDFNGYCKYSVLFYGYYNNQRTIGFLKFRLPLSYLMVGIGTFGYSLMVVIRTMAKNADVGGGDGEDNEFTFAWKMFTSWDYLIGNAETADNKYASITTSFKESIVDEQENQKDENIHLRRFLRVLANFLITCTLGGSGYLIYFVVKRSQEFQNMDNLSWYEKNELEIIMSLLGLVGPMLFETIAELEEYHPRIALKWQLGRIFALFLGNLYTFLLALFDEVNAKLEEEESIKNASIWFLKEYYANYTANNPNDTGTPPPINPADAIRGPCWETTVGVEFVKLTISDIQVTYLTILIGDFLRAFIVRFLNYCWCWDLEAGWPSYGEFDISGNVLGLVFNQGMIWMGAFYAPGLVGINVLRLLSSMYYQCWAVMACNVPHERVFKASKSNNFYMGLLLLILFLSLLPVVYTIMSLPPSFDCGPFSGKERMFDVVMETIDLDLPAFMGTLFGYVANPGLVISAVLLMVLAIYYLNSVSEAYKNSNNELKKKMQMARDEEKNRRNNKDSTNQVMKDLEDLLPNRPPTPPSPRENIAEKNQGQGGKSAKVKPGTAGGVHLQKDVSLASANPNARGPVTRAPGPRGPGPLPGQPGAGRGQGPPPRRQ

Target ORF information:

RefSeq Version	NM_001302237.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane channel-like 2a (tmc2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001302237.1

ORF Insert Sequence:

ATGCCAAAGA AAAGTGATAC AACAAGAAAA CTGGAAGATG TGGGAATAGA AATTGATGGG 
GATGTGGACA GTGCTGAAGA GGACAAGAAA TCCAAAGGCA AGGGTGGTAA AAAAGCAGCA 
GGCGGTAAGC GAGGTAAAGC CAGTGAGGAT GGTGAGGATG AAGATGAGGA TGATAAACCC 
CCCAAAGGTC GTCGGGCAGC CAATAAAAAG AAACCAGCAC CAGTAGATGA GGAAGACAGT 
GATGATGATA TTCCACAAAG GAGATCTGCT GGGAACAGGA GACGAGGAAA TGTGCGAGAG 
AGAGGAGATG GAGACAAAAA GAAGTCTGGC AAAAAAGGCA GGAGAGGTGG AAAAAAGAAT 
GAAAAAGGGA AAGGAAAAGA CAGTGACAAA GACAGTGACA AAGATGAAAA GAAGAAAAAG 
AACAGTAGTG GTGATGAATC CGATTCAGAT GAAGAGGATG AGTCCATGTC TGAAGGAGAA 
ATGGCCAAAC TCATGGAGGA GGTGGAGGAA AAGAAGAAAT TAATAGCAAA CATCAGGAAC 
AAGCCCTGGC GAATGAGAAG ACGACTTAAA GTCCTCAAGG AGGCTCAGCA GTTTGTGGAT 
AAATTTGAAG GTGCTTTGGG CAAAGGGAAA GGCAGAAAGC TGTATGCCTA CAAAGTTATG 
ATGATGAAGA AATGGATAAA GTTTAAAAGG GATTTTGAAA ATTTTAGAAC GGCTTGCATC 
CCATGGGAGC GGAAAATAAA GGAAGTTGAG AGTCACTTTG GCTCCTCTGT TGCTTCATAC 
TTTATTTTCC TCCGTTGGAT GTATGGCATG AACTTGGTCC TCTTCAGCTT AACTTTTGGC 
CTGGTTGTGA TACCAGAGGT TCTCATGGGA CTTCCATATG GGTCCATTCC TCGGAAAACT 
GTGCCGAGAG AAGACCAAGA TACTGCAATG GACTATTCTG TTCTTACGGA TTTCAATGGT 
TACTGCAAGT ACTCAGTACT TTTCTATGGC TACTATAATA ACCAGAGAAC CATTGGCTTC 
CTGAAGTTTC GTCTCCCTCT GTCCTACCTC ATGGTGGGCA TTGGGACCTT CGGGTATAGC 
CTCATGGTTG TGATCAGAAC GATGGCTAAA AACGCAGATG TGGGGGGAGG GGACGGAGAA 
GACAATGAGT TCACCTTTGC CTGGAAGATG TTCACCAGCT GGGATTATCT TATTGGGAAC 
GCTGAGACAG CTGACAATAA ATACGCCTCC ATCACCACCA GCTTCAAAGA ATCCATTGTG 
GATGAGCAGG AGAACCAAAA GGATGAAAAC ATTCATCTGA GAAGATTCTT ACGTGTCCTA 
GCCAACTTTC TCATCACCTG TACATTGGGT GGAAGTGGCT ATCTCATCTA TTTTGTAGTC 
AAACGCTCTC AAGAGTTTCA AAACATGGAC AACCTCTCAT GGTATGAGAA GAATGAGCTT 
GAGATCATCA TGTCTCTGTT GGGACTGGTG GGTCCAATGC TCTTTGAGAC CATCGCAGAG 
TTGGAGGAGT ACCATCCACG TATCGCTCTC AAGTGGCAAC TTGGACGTAT ATTTGCACTC 
TTTCTTGGCA ATCTCTACAC ATTTTTGCTG GCTCTGTTTG ATGAGGTCAA TGCAAAACTT 
GAAGAAGAGG AGTCTATAAA AAATGCCAGC ATTTGGTTCT TGAAGGAATA CTATGCCAAC 
TACACAGCCA ACAACCCAAA TGACACAGGC ACTCCACCTC CTATCAACCC AGCGGATGCC 
ATCAGAGGCC CATGCTGGGA AACCACAGTG GGAGTAGAGT TTGTGAAGCT GACTATATCA 
GATATACAGG TGACATATCT GACCATCCTC ATCGGTGATT TCTTGAGAGC GTTCATTGTA 
CGTTTCCTGA ACTATTGCTG GTGCTGGGAT TTGGAAGCCG GATGGCCTTC CTATGGTGAA 
TTTGACATAA GCGGCAATGT GCTTGGTTTG GTATTTAACC AAGGAATGAT CTGGATGGGG 
GCATTCTATG CTCCCGGTCT TGTAGGCATC AATGTTCTGC GGCTCCTTTC ATCTATGTAC 
TATCAATGCT GGGCTGTAAT GGCCTGTAAT GTCCCACATG AGAGGGTCTT TAAAGCTTCT 
AAATCCAATA ACTTCTACAT GGGCCTGTTA TTGCTCATCC TCTTTCTGAG TCTCCTACCT 
GTGGTTTACA CCATTATGTC TTTGCCACCA TCTTTTGATT GTGGACCTTT CAGTGGAAAG 
GAAAGAATGT TCGACGTTGT TATGGAGACC ATTGATCTGG ATCTACCAGC ATTTATGGGG 
ACTCTCTTTG GATATGTCGC AAACCCGGGA CTCGTTATAT CTGCAGTACT TCTCATGGTA 
CTGGCCATTT ACTACCTGAA TTCTGTGTCA GAGGCCTACA AGAACTCCAA CAATGAGCTA 
AAAAAGAAAA TGCAGATGGC AAGAGATGAG GAGAAGAACA GACGGAACAA CAAAGACAGC 
ACTAACCAAG TGATGAAAGA CCTAGAGGAT CTGCTTCCCA ATCGCCCTCC AACGCCTCCT 
TCTCCTCGAG AGAATATCGC AGAAAAAAAC CAGGGCCAGG GTGGAAAATC TGCAAAAGTT 
AAACCAGGCA CAGCTGGCGG TGTGCATCTG CAGAAAGATG TGTCACTCGC ATCAGCCAAT 
CCTAATGCAC GGGGGCCTGT GACACGAGCG CCAGGCCCCA GGGGTCCTGG ACCTCTACCT 
GGCCAACCAG GGGCAGGTAG GGGTCAAGGT CCACCCCCTA GACGGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A molecular basis for water motion detection by the mechanosensory lateral line of zebrafish.
Nature communications8(1)2234(2017 Dec)
Chou SW,Chen Z,Zhu S,Davis RW,Hu J,Liu L,Fernando CA,Kindig K,Brown WC,Stepanyan R,McDermott BM Jr

Integration of Tmc1/2 into the mechanotransduction complex in zebrafish hair cells is regulated by Transmembrane O-methyltransferase (Tomt).
eLife6(2017 May)
Erickson T,Morgan CP,Olt J,Hardy K,Busch-Nentwich E,Maeda R,Clemens R,Krey JF,Nechiporuk A,Barr-Gillespie PG,Marcotti W,Nicolson T

Identification of sensory hair-cell transcripts by thiouracil-tagging in zebrafish.
BMC genomics16842(2015 Oct)
Erickson T,Nicolson T

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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tmc2a cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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