pde8b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pde8b
Entrez Gene ID	571011
Full Name	phosphodiesterase 8B
Synonyms	si:dkeyp-1d11.1
General protein information	Preferred Names phosphodiesterase 8B Names high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009295181.2	XP_009293456.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X2
XM_009295180.3	XP_009293455.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X1
XM_021469002.1	XP_021324677.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X2
XM_009295180.2	XP_009293455.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X1
XM_009295181.3	XP_009293456.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PDE8B	XP_546056.4
Bos taurus (cattle)	PDE8B	XP_003586548.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007163	XP_308604.4
Gallus gallus (chicken)	PDE8B	XP_425218.4
Danio rerio (zebrafish)	pde8b	XP_005170307.1
Drosophila melanogaster (fruit fly)	Pde8	NP_001163274.1
Xenopus tropicalis (tropical clawed frog)	pde8b	XP_002940920.1
Pan troglodytes (chimpanzee)	PDE8B	XP_517695.4
Mus musculus (house mouse)	Pde8b	NP_758467.2
Rattus norvegicus (Norway rat)	Pde8b	NP_954889.1
Homo sapiens (human)	PDE8B	NP_003710.1

Gene Ontology

The following pde8b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pde8b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa46067	XM_009295180.3 Latest version!	Danio rerio phosphodiesterase 8B (pde8b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa46068	XM_009295181.3 Latest version!	Danio rerio phosphodiesterase 8B (pde8b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa25559	XM_021469002.1 Latest version!	Danio rerio phosphodiesterase 8B (pde8b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
ODa46067	XM_009295180.2	Danio rerio phosphodiesterase 8B (pde8b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa46068	XM_009295181.2	Danio rerio phosphodiesterase 8B (pde8b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pde8b ( XM_009295180.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293455.1 Danio rerio phosphodiesterase 8B (pde8b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa46067
Clone ID Related Accession (Same CDS sequence)	XM_009295180.3 , XM_009295180.2
Accession Version	XM_009295180.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295180.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCG CTCCCAGCAT TCACGTCTCG CAGAGCGGGG TGATCTACTG CCGCGACTCG 
GATGAGTCCA ACTCGCCGCA CCAGACCACT ACTATCTCGC AGGGCACCGC GACCACCCTG 
CACGGCCTCT TCATTAAGAC CGACGCGGCG GATACCATCC CCTCCGTGAT CGCGTACCAG 
ACCAGGCACA CACGGACCAA CTCCCACCGG GAGCGGAGAG AGAACAGCGG CGGACAGATG 
TGCACAGAGG CCGAGACCCA GACCAGCTGC ACCAGCGTCA AGGTGTCTGG TGCTGAGGAT 
CGATTGGGGC CGATGCGACT GAGCCATGAA CCCATACAGG TGTTACTGGT CTTCGCCAAA 
GAGGACAGTC AGAGCGATGC CTTCTGGTGG GCGTGCGAGA GGGCCGCTTT CAGGTGCAAC 
ATCGCACGCA CACCTGAGTC TGCGCTCGAG TGTTTCCTGG ACAAACACCA CGAGATCGTC 
ATCATCGATG GACGACACCC GCGATACTTT GAGGCCGAGG CCATCTGTCG GATGATTCGA 
GCAACCAAGC TGTCTGAACA CACAGTTATT TTGGCAGTGA TGCCCCAAAC TCCGTCAGAT 
CAGGAGGAAC CATCTGTCCT TCCTCTTCTC TCTGCTGGCT TCTCTAGACG GTACCTGGAG 
AACAGCAGTG TTTCAGCCTG CTATAATGAG TTAATCCAGC TGGAGCACGG AGAGGTTCGG 
GCTCAGTTCA AGCTGAGGGC TTGTAACGCT GTGTTTACTG CACTAGAACA GTGTCAGGAG 
GCCGTGGAGA TCAGCAGTGA GGATCATGTC ATACAATATG TAAATCCAGC GTTTGAAAAG 
ATGATGGGTT ATCATCGAGG AGAACTGATC GGTAAGGAGC TGACCGAACT GCCCAGGAGT 
GACAAGAACA GCGCCGACCT GCTGGACACC ATCAACACCT GCATCAAGAA AGGAAAGGAA 
TGGCAGGGGA TCTATTTCTC AAGGAGGAAG TCAGGGGACA GTATTCAGCA ATATGTCAAA 
ATCACTCCTG TGGTTGGCCA GGGAGGAAAA ATCCGTCATT TCGTGTCCAT CAAGAGGCCT 
TACAGTGACA ACCACAGACA GGTTCACAAG CTGAGCAAAG AAGAGAAATG CTGTGGTGAA 
CACTCACAAT CAGAATGCAA CTCCTTGCGT CACAAGGACC GGAGGAAAGA GTCTGTGGAC 
GTCAGATCAA TCAGCTCTCA CAGCAGCGAC GCTCCTAGTC TACAGAACCG CAGATATTCC 
TCTATGGCCC GAATTCATTC GATGACGATT GAGGCTCCGA TCACTAAGGT GATAAATATC 
ATTAACGCCG CGCAGGAGAG CAGTCCGGTG ACGGTAGCGG AAGCTCTGGA TCGTGTTCTG 
GAGATTTTGA GGACCACTGA ACTCTACTCA CCCCAGCTGG CGTCAAAAGA GGACGATCCC 
CACACCAATG ACCTGGTCGG GGGACTCATG AGTGACGGAC TCAGAAGGCT CTCCGGGAAC 
GAGTACATCT TCTCCAAAAG TGCTTTACAA AACATGAGTC AGAGTCAGCT GGGTGTGCCC 
GTCCCGCTGA ATGACATCCC GCCGTCCATC GCAGAGCTGC TGAATGAAGA GGAGCAGTGG 
GAGTTCAACA TCCTGGAGCT GGAGGCAGCC ACACACAAGA GGCCACTGTC GTACCTGGGT 
CTGAAGATCT TCTCTGCGTT TGGCGTGTGC GAGTTTCTGA ACTGCTCCGA GTCCACGCTG 
CGCTTGTGGC TGCAGGTGAT CGAGACCAAC TATCACTCCT CAAACTCCTA CCATAACTCC 
ACACACGCCG CTGACGTGCT GCACGCCACC GCTTACTTCC TGCGCAAGGA GAGAGTCAAG 
GCCAGTCTTG ACCAGCTAGA TGAAGTTGCC GCTCTGTTAG CTGCGACAGT TCATGACGTG 
GATCATCCGG GCCGCACAAA CTCATTCCTG TGCAACGCAG GCAGTGAATT GGCCATTCTG 
TACAACGATA CAGCCGTACT GGAGAGTCAC CATGCTGCTC TCGCCTTCCA GCTCACCGTA 
CGGGACAGCA AGTGCAACAT CTTCAAGAAC ATGGAAAGGA CTCAGTTTCG GACGCTGCGG 
CAGGCCATAA TCGACATGGT ATTGGCAACA GAAATGACTC GACACTTCGA ACACGTCAAT 
AAGTTCGTCA ACAGCATCAA CAAACCCATG AGTGCCATAG AAGAGACAAG TTCCAATAGT 
GAAGGCAGTG ATTGTGAGGG TCAGGCCAGT ATTCGTAACT CTCCTGAGAA TCGGCTACTG 
ATTAAACGAA TGCTGATTAA ATGTGCGGAT GTGGCCAATC CCTGTCGACC GCTGGAGCTC 
TGCATTGAAT GGGCCGGTCG CATTTCAGAG GAGTATTTCG CTCAGACTGA TGAAGAGAAA 
AGACAGGGTT TGCCGGTTGT CATGCCAGTG TTTGACCGGA ACACCTGCAG CATTCCTAAA 
TCCCAGATCT CCTTCATCGA CTATTTCATC ACAGACATGT TTGATGCATG GGATGCCTTT 
GCCAGTTTGC CAGGTCTGAT GGAGCATTTG GCAGAGAACT ACAAATACTG GAAGAACTTG 
GATGAGATGA AATGTAAAAG CCTTCGGCCT CCACCTCCTC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293455.1
CDS	457..3081
Translation	MGCAPSIHVSQSGVIYCRDSDESNSPHQTTTISQGTATTLHGLFIKTDAADTIPSVIAYQTRHTRTNSHRERRENSGGQMCTEAETQTSCTSVKVSGAEDRLGPMRLSHEPIQVLLVFAKEDSQSDAFWWACERAAFRCNIARTPESALECFLDKHHEIVIIDGRHPRYFEAEAICRMIRATKLSEHTVILAVMPQTPSDQEEPSVLPLLSAGFSRRYLENSSVSACYNELIQLEHGEVRAQFKLRACNAVFTALEQCQEAVEISSEDHVIQYVNPAFEKMMGYHRGELIGKELTELPRSDKNSADLLDTINTCIKKGKEWQGIYFSRRKSGDSIQQYVKITPVVGQGGKIRHFVSIKRPYSDNHRQVHKLSKEEKCCGEHSQSECNSLRHKDRRKESVDVRSISSHSSDAPSLQNRRYSSMARIHSMTIEAPITKVINIINAAQESSPVTVAEALDRVLEILRTTELYSPQLASKEDDPHTNDLVGGLMSDGLRRLSGNEYIFSKSALQNMSQSQLGVPVPLNDIPPSIAELLNEEEQWEFNILELEAATHKRPLSYLGLKIFSAFGVCEFLNCSESTLRLWLQVIETNYHSSNSYHNSTHAADVLHATAYFLRKERVKASLDQLDEVAALLAATVHDVDHPGRTNSFLCNAGSELAILYNDTAVLESHHAALAFQLTVRDSKCNIFKNMERTQFRTLRQAIIDMVLATEMTRHFEHVNKFVNSINKPMSAIEETSSNSEGSDCEGQASIRNSPENRLLIKRMLIKCADVANPCRPLELCIEWAGRISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFASLPGLMEHLAENYKYWKNLDEMKCKSLRPPPPP

Target ORF information:

RefSeq Version	XM_009295180.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphodiesterase 8B (pde8b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295180.3

ORF Insert Sequence:

ATGGGCTGCG CTCCCAGCAT TCACGTCTCG CAGAGCGGGG TGATCTACTG CCGCGACTCG 
GATGAGTCCA ACTCGCCGCA CCAGACCACT ACTATCTCGC AGGGCACCGC GACCACCCTG 
CACGGCCTCT TCATTAAGAC CGACGCGGCG GATACCATCC CCTCCGTGAT CGCGTACCAG 
ACCAGGCACA CACGGACCAA CTCCCACCGG GAGCGGAGAG AGAACAGCGG CGGACAGATG 
TGCACAGAGG CCGAGACCCA GACCAGCTGC ACCAGCGTCA AGGTGTCTGG TGCTGAGGAT 
CGATTGGGGC CGATGCGACT GAGCCATGAA CCCATACAGG TGTTACTGGT CTTCGCCAAA 
GAGGACAGTC AGAGCGATGC CTTCTGGTGG GCGTGCGAGA GGGCCGCTTT CAGGTGCAAC 
ATCGCACGCA CACCTGAGTC TGCGCTCGAG TGTTTCCTGG ACAAACACCA CGAGATCGTC 
ATCATCGATG GACGACACCC GCGATACTTT GAGGCCGAGG CCATCTGTCG GATGATTCGA 
GCAACCAAGC TGTCTGAACA CACAGTTATT TTGGCAGTGA TGCCCCAAAC TCCGTCAGAT 
CAGGAGGAAC CATCTGTCCT TCCTCTTCTC TCTGCTGGCT TCTCTAGACG GTACCTGGAG 
AACAGCAGTG TTTCAGCCTG CTATAATGAG TTAATCCAGC TGGAGCACGG AGAGGTTCGG 
GCTCAGTTCA AGCTGAGGGC TTGTAACGCT GTGTTTACTG CACTAGAACA GTGTCAGGAG 
GCCGTGGAGA TCAGCAGTGA GGATCATGTC ATACAATATG TAAATCCAGC GTTTGAAAAG 
ATGATGGGTT ATCATCGAGG AGAACTGATC GGTAAGGAGC TGACCGAACT GCCCAGGAGT 
GACAAGAACA GCGCCGACCT GCTGGACACC ATCAACACCT GCATCAAGAA AGGAAAGGAA 
TGGCAGGGGA TCTATTTCTC AAGGAGGAAG TCAGGGGACA GTATTCAGCA ATATGTCAAA 
ATCACTCCTG TGGTTGGCCA GGGAGGAAAA ATCCGTCATT TCGTGTCCAT CAAGAGGCCT 
TACAGTGACA ACCACAGACA GGTTCACAAG CTGAGCAAAG AAGAGAAATG CTGTGGTGAA 
CACTCACAAT CAGAATGCAA CTCCTTGCGT CACAAGGACC GGAGGAAAGA GTCTGTGGAC 
GTCAGATCAA TCAGCTCTCA CAGCAGCGAC GCTCCTAGTC TACAGAACCG CAGATATTCC 
TCTATGGCCC GAATTCATTC GATGACGATT GAGGCTCCGA TCACTAAGGT GATAAATATC 
ATTAACGCCG CGCAGGAGAG CAGTCCGGTG ACGGTAGCGG AAGCTCTGGA TCGTGTTCTG 
GAGATTTTGA GGACCACTGA ACTCTACTCA CCCCAGCTGG CGTCAAAAGA GGACGATCCC 
CACACCAATG ACCTGGTCGG GGGACTCATG AGTGACGGAC TCAGAAGGCT CTCCGGGAAC 
GAGTACATCT TCTCCAAAAG TGCTTTACAA AACATGAGTC AGAGTCAGCT GGGTGTGCCC 
GTCCCGCTGA ATGACATCCC GCCGTCCATC GCAGAGCTGC TGAATGAAGA GGAGCAGTGG 
GAGTTCAACA TCCTGGAGCT GGAGGCAGCC ACACACAAGA GGCCACTGTC GTACCTGGGT 
CTGAAGATCT TCTCTGCGTT TGGCGTGTGC GAGTTTCTGA ACTGCTCCGA GTCCACGCTG 
CGCTTGTGGC TGCAGGTGAT CGAGACCAAC TATCACTCCT CAAACTCCTA CCATAACTCC 
ACACACGCCG CTGACGTGCT GCACGCCACC GCTTACTTCC TGCGCAAGGA GAGAGTCAAG 
GCCAGTCTTG ACCAGCTAGA TGAAGTTGCC GCTCTGTTAG CTGCGACAGT TCATGACGTG 
GATCATCCGG GCCGCACAAA CTCATTCCTG TGCAACGCAG GCAGTGAATT GGCCATTCTG 
TACAACGATA CAGCCGTACT GGAGAGTCAC CATGCTGCTC TCGCCTTCCA GCTCACCGTA 
CGGGACAGCA AGTGCAACAT CTTCAAGAAC ATGGAAAGGA CTCAGTTTCG GACGCTGCGG 
CAGGCCATAA TCGACATGGT ATTGGCAACA GAAATGACTC GACACTTCGA ACACGTCAAT 
AAGTTCGTCA ACAGCATCAA CAAACCCATG AGTGCCATAG AAGAGACAAG TTCCAATAGT 
GAAGGCAGTG ATTGTGAGGG TCAGGCCAGT ATTCGTAACT CTCCTGAGAA TCGGCTACTG 
ATTAAACGAA TGCTGATTAA ATGTGCGGAT GTGGCCAATC CCTGTCGACC GCTGGAGCTC 
TGCATTGAAT GGGCCGGTCG CATTTCAGAG GAGTATTTCG CTCAGACTGA TGAAGAGAAA 
AGACAGGGTT TGCCGGTTGT CATGCCAGTG TTTGACCGGA ACACCTGCAG CATTCCTAAA 
TCCCAGATCT CCTTCATCGA CTATTTCATC ACAGACATGT TTGATGCATG GGATGCCTTT 
GCCAGTTTGC CAGGTCTGAT GGAGCATTTG GCAGAGAACT ACAAATACTG GAAGAACTTG 
GATGAGATGA AATGTAAAAG CCTTCGGCCT CCACCTCCTC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde8b ( XM_009295180.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293455.1 Danio rerio phosphodiesterase 8B (pde8b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa46067
Clone ID Related Accession (Same CDS sequence)	XM_009295180.3 , XM_009295180.2
Accession Version	XM_009295180.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295180.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCG CTCCCAGCAT TCACGTCTCG CAGAGCGGGG TGATCTACTG CCGCGACTCG 
GATGAGTCCA ACTCGCCGCA CCAGACCACT ACTATCTCGC AGGGCACCGC GACCACCCTG 
CACGGCCTCT TCATTAAGAC CGACGCGGCG GATACCATCC CCTCCGTGAT CGCGTACCAG 
ACCAGGCACA CACGGACCAA CTCCCACCGG GAGCGGAGAG AGAACAGCGG CGGACAGATG 
TGCACAGAGG CCGAGACCCA GACCAGCTGC ACCAGCGTCA AGGTGTCTGG TGCTGAGGAT 
CGATTGGGGC CGATGCGACT GAGCCATGAA CCCATACAGG TGTTACTGGT CTTCGCCAAA 
GAGGACAGTC AGAGCGATGC CTTCTGGTGG GCGTGCGAGA GGGCCGCTTT CAGGTGCAAC 
ATCGCACGCA CACCTGAGTC TGCGCTCGAG TGTTTCCTGG ACAAACACCA CGAGATCGTC 
ATCATCGATG GACGACACCC GCGATACTTT GAGGCCGAGG CCATCTGTCG GATGATTCGA 
GCAACCAAGC TGTCTGAACA CACAGTTATT TTGGCAGTGA TGCCCCAAAC TCCGTCAGAT 
CAGGAGGAAC CATCTGTCCT TCCTCTTCTC TCTGCTGGCT TCTCTAGACG GTACCTGGAG 
AACAGCAGTG TTTCAGCCTG CTATAATGAG TTAATCCAGC TGGAGCACGG AGAGGTTCGG 
GCTCAGTTCA AGCTGAGGGC TTGTAACGCT GTGTTTACTG CACTAGAACA GTGTCAGGAG 
GCCGTGGAGA TCAGCAGTGA GGATCATGTC ATACAATATG TAAATCCAGC GTTTGAAAAG 
ATGATGGGTT ATCATCGAGG AGAACTGATC GGTAAGGAGC TGACCGAACT GCCCAGGAGT 
GACAAGAACA GCGCCGACCT GCTGGACACC ATCAACACCT GCATCAAGAA AGGAAAGGAA 
TGGCAGGGGA TCTATTTCTC AAGGAGGAAG TCAGGGGACA GTATTCAGCA ATATGTCAAA 
ATCACTCCTG TGGTTGGCCA GGGAGGAAAA ATCCGTCATT TCGTGTCCAT CAAGAGGCCT 
TACAGTGACA ACCACAGACA GGTTCACAAG CTGAGCAAAG AAGAGAAATG CTGTGGTGAA 
CACTCACAAT CAGAATGCAA CTCCTTGCGT CACAAGGACC GGAGGAAAGA GTCTGTGGAC 
GTCAGATCAA TCAGCTCTCA CAGCAGCGAC GCTCCTAGTC TACAGAACCG CAGATATTCC 
TCTATGGCCC GAATTCATTC GATGACGATT GAGGCTCCGA TCACTAAGGT GATAAATATC 
ATTAACGCCG CGCAGGAGAG CAGTCCGGTG ACGGTAGCGG AAGCTCTGGA TCGTGTTCTG 
GAGATTTTGA GGACCACTGA ACTCTACTCA CCCCAGCTGG CGTCAAAAGA GGACGATCCC 
CACACCAATG ACCTGGTCGG GGGACTCATG AGTGACGGAC TCAGAAGGCT CTCCGGGAAC 
GAGTACATCT TCTCCAAAAG TGCTTTACAA AACATGAGTC AGAGTCAGCT GGGTGTGCCC 
GTCCCGCTGA ATGACATCCC GCCGTCCATC GCAGAGCTGC TGAATGAAGA GGAGCAGTGG 
GAGTTCAACA TCCTGGAGCT GGAGGCAGCC ACACACAAGA GGCCACTGTC GTACCTGGGT 
CTGAAGATCT TCTCTGCGTT TGGCGTGTGC GAGTTTCTGA ACTGCTCCGA GTCCACGCTG 
CGCTTGTGGC TGCAGGTGAT CGAGACCAAC TATCACTCCT CAAACTCCTA CCATAACTCC 
ACACACGCCG CTGACGTGCT GCACGCCACC GCTTACTTCC TGCGCAAGGA GAGAGTCAAG 
GCCAGTCTTG ACCAGCTAGA TGAAGTTGCC GCTCTGTTAG CTGCGACAGT TCATGACGTG 
GATCATCCGG GCCGCACAAA CTCATTCCTG TGCAACGCAG GCAGTGAATT GGCCATTCTG 
TACAACGATA CAGCCGTACT GGAGAGTCAC CATGCTGCTC TCGCCTTCCA GCTCACCGTA 
CGGGACAGCA AGTGCAACAT CTTCAAGAAC ATGGAAAGGA CTCAGTTTCG GACGCTGCGG 
CAGGCCATAA TCGACATGGT ATTGGCAACA GAAATGACTC GACACTTCGA ACACGTCAAT 
AAGTTCGTCA ACAGCATCAA CAAACCCATG AGTGCCATAG AAGAGACAAG TTCCAATAGT 
GAAGGCAGTG ATTGTGAGGG TCAGGCCAGT ATTCGTAACT CTCCTGAGAA TCGGCTACTG 
ATTAAACGAA TGCTGATTAA ATGTGCGGAT GTGGCCAATC CCTGTCGACC GCTGGAGCTC 
TGCATTGAAT GGGCCGGTCG CATTTCAGAG GAGTATTTCG CTCAGACTGA TGAAGAGAAA 
AGACAGGGTT TGCCGGTTGT CATGCCAGTG TTTGACCGGA ACACCTGCAG CATTCCTAAA 
TCCCAGATCT CCTTCATCGA CTATTTCATC ACAGACATGT TTGATGCATG GGATGCCTTT 
GCCAGTTTGC CAGGTCTGAT GGAGCATTTG GCAGAGAACT ACAAATACTG GAAGAACTTG 
GATGAGATGA AATGTAAAAG CCTTCGGCCT CCACCTCCTC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293455.1
CDS	438..3062
Translation	MGCAPSIHVSQSGVIYCRDSDESNSPHQTTTISQGTATTLHGLFIKTDAADTIPSVIAYQTRHTRTNSHRERRENSGGQMCTEAETQTSCTSVKVSGAEDRLGPMRLSHEPIQVLLVFAKEDSQSDAFWWACERAAFRCNIARTPESALECFLDKHHEIVIIDGRHPRYFEAEAICRMIRATKLSEHTVILAVMPQTPSDQEEPSVLPLLSAGFSRRYLENSSVSACYNELIQLEHGEVRAQFKLRACNAVFTALEQCQEAVEISSEDHVIQYVNPAFEKMMGYHRGELIGKELTELPRSDKNSADLLDTINTCIKKGKEWQGIYFSRRKSGDSIQQYVKITPVVGQGGKIRHFVSIKRPYSDNHRQVHKLSKEEKCCGEHSQSECNSLRHKDRRKESVDVRSISSHSSDAPSLQNRRYSSMARIHSMTIEAPITKVINIINAAQESSPVTVAEALDRVLEILRTTELYSPQLASKEDDPHTNDLVGGLMSDGLRRLSGNEYIFSKSALQNMSQSQLGVPVPLNDIPPSIAELLNEEEQWEFNILELEAATHKRPLSYLGLKIFSAFGVCEFLNCSESTLRLWLQVIETNYHSSNSYHNSTHAADVLHATAYFLRKERVKASLDQLDEVAALLAATVHDVDHPGRTNSFLCNAGSELAILYNDTAVLESHHAALAFQLTVRDSKCNIFKNMERTQFRTLRQAIIDMVLATEMTRHFEHVNKFVNSINKPMSAIEETSSNSEGSDCEGQASIRNSPENRLLIKRMLIKCADVANPCRPLELCIEWAGRISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFASLPGLMEHLAENYKYWKNLDEMKCKSLRPPPPP

Target ORF information:

RefSeq Version	XM_009295180.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphodiesterase 8B (pde8b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295180.2

ORF Insert Sequence:

ATGGGCTGCG CTCCCAGCAT TCACGTCTCG CAGAGCGGGG TGATCTACTG CCGCGACTCG 
GATGAGTCCA ACTCGCCGCA CCAGACCACT ACTATCTCGC AGGGCACCGC GACCACCCTG 
CACGGCCTCT TCATTAAGAC CGACGCGGCG GATACCATCC CCTCCGTGAT CGCGTACCAG 
ACCAGGCACA CACGGACCAA CTCCCACCGG GAGCGGAGAG AGAACAGCGG CGGACAGATG 
TGCACAGAGG CCGAGACCCA GACCAGCTGC ACCAGCGTCA AGGTGTCTGG TGCTGAGGAT 
CGATTGGGGC CGATGCGACT GAGCCATGAA CCCATACAGG TGTTACTGGT CTTCGCCAAA 
GAGGACAGTC AGAGCGATGC CTTCTGGTGG GCGTGCGAGA GGGCCGCTTT CAGGTGCAAC 
ATCGCACGCA CACCTGAGTC TGCGCTCGAG TGTTTCCTGG ACAAACACCA CGAGATCGTC 
ATCATCGATG GACGACACCC GCGATACTTT GAGGCCGAGG CCATCTGTCG GATGATTCGA 
GCAACCAAGC TGTCTGAACA CACAGTTATT TTGGCAGTGA TGCCCCAAAC TCCGTCAGAT 
CAGGAGGAAC CATCTGTCCT TCCTCTTCTC TCTGCTGGCT TCTCTAGACG GTACCTGGAG 
AACAGCAGTG TTTCAGCCTG CTATAATGAG TTAATCCAGC TGGAGCACGG AGAGGTTCGG 
GCTCAGTTCA AGCTGAGGGC TTGTAACGCT GTGTTTACTG CACTAGAACA GTGTCAGGAG 
GCCGTGGAGA TCAGCAGTGA GGATCATGTC ATACAATATG TAAATCCAGC GTTTGAAAAG 
ATGATGGGTT ATCATCGAGG AGAACTGATC GGTAAGGAGC TGACCGAACT GCCCAGGAGT 
GACAAGAACA GCGCCGACCT GCTGGACACC ATCAACACCT GCATCAAGAA AGGAAAGGAA 
TGGCAGGGGA TCTATTTCTC AAGGAGGAAG TCAGGGGACA GTATTCAGCA ATATGTCAAA 
ATCACTCCTG TGGTTGGCCA GGGAGGAAAA ATCCGTCATT TCGTGTCCAT CAAGAGGCCT 
TACAGTGACA ACCACAGACA GGTTCACAAG CTGAGCAAAG AAGAGAAATG CTGTGGTGAA 
CACTCACAAT CAGAATGCAA CTCCTTGCGT CACAAGGACC GGAGGAAAGA GTCTGTGGAC 
GTCAGATCAA TCAGCTCTCA CAGCAGCGAC GCTCCTAGTC TACAGAACCG CAGATATTCC 
TCTATGGCCC GAATTCATTC GATGACGATT GAGGCTCCGA TCACTAAGGT GATAAATATC 
ATTAACGCCG CGCAGGAGAG CAGTCCGGTG ACGGTAGCGG AAGCTCTGGA TCGTGTTCTG 
GAGATTTTGA GGACCACTGA ACTCTACTCA CCCCAGCTGG CGTCAAAAGA GGACGATCCC 
CACACCAATG ACCTGGTCGG GGGACTCATG AGTGACGGAC TCAGAAGGCT CTCCGGGAAC 
GAGTACATCT TCTCCAAAAG TGCTTTACAA AACATGAGTC AGAGTCAGCT GGGTGTGCCC 
GTCCCGCTGA ATGACATCCC GCCGTCCATC GCAGAGCTGC TGAATGAAGA GGAGCAGTGG 
GAGTTCAACA TCCTGGAGCT GGAGGCAGCC ACACACAAGA GGCCACTGTC GTACCTGGGT 
CTGAAGATCT TCTCTGCGTT TGGCGTGTGC GAGTTTCTGA ACTGCTCCGA GTCCACGCTG 
CGCTTGTGGC TGCAGGTGAT CGAGACCAAC TATCACTCCT CAAACTCCTA CCATAACTCC 
ACACACGCCG CTGACGTGCT GCACGCCACC GCTTACTTCC TGCGCAAGGA GAGAGTCAAG 
GCCAGTCTTG ACCAGCTAGA TGAAGTTGCC GCTCTGTTAG CTGCGACAGT TCATGACGTG 
GATCATCCGG GCCGCACAAA CTCATTCCTG TGCAACGCAG GCAGTGAATT GGCCATTCTG 
TACAACGATA CAGCCGTACT GGAGAGTCAC CATGCTGCTC TCGCCTTCCA GCTCACCGTA 
CGGGACAGCA AGTGCAACAT CTTCAAGAAC ATGGAAAGGA CTCAGTTTCG GACGCTGCGG 
CAGGCCATAA TCGACATGGT ATTGGCAACA GAAATGACTC GACACTTCGA ACACGTCAAT 
AAGTTCGTCA ACAGCATCAA CAAACCCATG AGTGCCATAG AAGAGACAAG TTCCAATAGT 
GAAGGCAGTG ATTGTGAGGG TCAGGCCAGT ATTCGTAACT CTCCTGAGAA TCGGCTACTG 
ATTAAACGAA TGCTGATTAA ATGTGCGGAT GTGGCCAATC CCTGTCGACC GCTGGAGCTC 
TGCATTGAAT GGGCCGGTCG CATTTCAGAG GAGTATTTCG CTCAGACTGA TGAAGAGAAA 
AGACAGGGTT TGCCGGTTGT CATGCCAGTG TTTGACCGGA ACACCTGCAG CATTCCTAAA 
TCCCAGATCT CCTTCATCGA CTATTTCATC ACAGACATGT TTGATGCATG GGATGCCTTT 
GCCAGTTTGC CAGGTCTGAT GGAGCATTTG GCAGAGAACT ACAAATACTG GAAGAACTTG 
GATGAGATGA AATGTAAAAG CCTTCGGCCT CCACCTCCTC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde8b ( XM_009295181.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293456.1 Danio rerio phosphodiesterase 8B (pde8b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa46068
Clone ID Related Accession (Same CDS sequence)	XM_009295181.2 , XM_009295181.3
Accession Version	XM_009295181.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295181.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACTCC AGGGCTCGGA GATGGTGTCT GGTGCTGAGG ATCGATTGGG GCCGATGCGA 
CTGAGCCATG AACCCATACA GGTGTTACTG GTCTTCGCCA AAGAGGACAG TCAGAGCGAT 
GCCTTCTGGT GGGCGTGCGA GAGGGCCGCT TTCAGGTGCA ACATCGCACG CACACCTGAG 
TCTGCGCTCG AGTGTTTCCT GGACAAACAC CACGAGATCG TCATCATCGA TGGACGACAC 
CCGCGATACT TTGAGGCCGA GGCCATCTGT CGGATGATTC GAGCAACCAA GCTGTCTGAA 
CACACAGTTA TTTTGGCAGT GATGCCCCAA ACTCCGTCAG ATCAGGAGGA ACCATCTGTC 
CTTCCTCTTC TCTCTGCTGG CTTCTCTAGA CGGTACCTGG AGAACAGCAG TGTTTCAGCC 
TGCTATAATG AGTTAATCCA GCTGGAGCAC GGAGAGGTTC GGGCTCAGTT CAAGCTGAGG 
GCTTGTAACG CTGTGTTTAC TGCACTAGAA CAGTGTCAGG AGGCCGTGGA GATCAGCAGT 
GAGGATCATG TCATACAATA TGTAAATCCA GCGTTTGAAA AGATGATGGG TTATCATCGA 
GGAGAACTGA TCGGTAAGGA GCTGACCGAA CTGCCCAGGA GTGACAAGAA CAGCGCCGAC 
CTGCTGGACA CCATCAACAC CTGCATCAAG AAAGGAAAGG AATGGCAGGG GATCTATTTC 
TCAAGGAGGA AGTCAGGGGA CAGTATTCAG CAATATGTCA AAATCACTCC TGTGGTTGGC 
CAGGGAGGAA AAATCCGTCA TTTCGTGTCC ATCAAGAGGC CTTACAGTGA CAACCACAGA 
CAGGTTCACA AGCTGAGCAA AGAAGAGAAA TGCTGTGGTG AACACTCACA ATCAGAATGC 
AACTCCTTGC GTCACAAGGA CCGGAGGAAA GAGTCTGTGG ACGTCAGATC AATCAGCTCT 
CACAGCAGCG ACGCTCCTAG TCTACAGAAC CGCAGATATT CCTCTATGGC CCGAATTCAT 
TCGATGACGA TTGAGGCTCC GATCACTAAG GTGATAAATA TCATTAACGC CGCGCAGGAG 
AGCAGTCCGG TGACGGTAGC GGAAGCTCTG GATCGTGTTC TGGAGATTTT GAGGACCACT 
GAACTCTACT CACCCCAGCT GGCGTCAAAA GAGGACGATC CCCACACCAA TGACCTGGTC 
GGGGGACTCA TGAGTGACGG ACTCAGAAGG CTCTCCGGGA ACGAGTACAT CTTCTCCAAA 
AGTGCTTTAC AAAACATGAG TCAGAGTCAG CTGGGTGTGC CCGTCCCGCT GAATGACATC 
CCGCCGTCCA TCGCAGAGCT GCTGAATGAA GAGGAGCAGT GGGAGTTCAA CATCCTGGAG 
CTGGAGGCAG CCACACACAA GAGGCCACTG TCGTACCTGG GTCTGAAGAT CTTCTCTGCG 
TTTGGCGTGT GCGAGTTTCT GAACTGCTCC GAGTCCACGC TGCGCTTGTG GCTGCAGGTG 
ATCGAGACCA ACTATCACTC CTCAAACTCC TACCATAACT CCACACACGC CGCTGACGTG 
CTGCACGCCA CCGCTTACTT CCTGCGCAAG GAGAGAGTCA AGGCCAGTCT TGACCAGCTA 
GATGAAGTTG CCGCTCTGTT AGCTGCGACA GTTCATGACG TGGATCATCC GGGCCGCACA 
AACTCATTCC TGTGCAACGC AGGCAGTGAA TTGGCCATTC TGTACAACGA TACAGCCGTA 
CTGGAGAGTC ACCATGCTGC TCTCGCCTTC CAGCTCACCG TACGGGACAG CAAGTGCAAC 
ATCTTCAAGA ACATGGAAAG GACTCAGTTT CGGACGCTGC GGCAGGCCAT AATCGACATG 
GTATTGGCAA CAGAAATGAC TCGACACTTC GAACACGTCA ATAAGTTCGT CAACAGCATC 
AACAAACCCA TGAGTGCCAT AGAAGAGACA AGTTCCAATA GTGAAGGCAG TGATTGTGAG 
GGTCAGGCCA GTATTCGTAA CTCTCCTGAG AATCGGCTAC TGATTAAACG AATGCTGATT 
AAATGTGCGG ATGTGGCCAA TCCCTGTCGA CCGCTGGAGC TCTGCATTGA ATGGGCCGGT 
CGCATTTCAG AGGAGTATTT CGCTCAGACT GATGAAGAGA AAAGACAGGG TTTGCCGGTT 
GTCATGCCAG TGTTTGACCG GAACACCTGC AGCATTCCTA AATCCCAGAT CTCCTTCATC 
GACTATTTCA TCACAGACAT GTTTGATGCA TGGGATGCCT TTGCCAGTTT GCCAGGTCTG 
ATGGAGCATT TGGCAGAGAA CTACAAATAC TGGAAGAACT TGGATGAGAT GAAATGTAAA 
AGCCTTCGGC CTCCACCTCC TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293456.1
CDS	328..2694
Translation	MLLQGSEMVSGAEDRLGPMRLSHEPIQVLLVFAKEDSQSDAFWWACERAAFRCNIARTPESALECFLDKHHEIVIIDGRHPRYFEAEAICRMIRATKLSEHTVILAVMPQTPSDQEEPSVLPLLSAGFSRRYLENSSVSACYNELIQLEHGEVRAQFKLRACNAVFTALEQCQEAVEISSEDHVIQYVNPAFEKMMGYHRGELIGKELTELPRSDKNSADLLDTINTCIKKGKEWQGIYFSRRKSGDSIQQYVKITPVVGQGGKIRHFVSIKRPYSDNHRQVHKLSKEEKCCGEHSQSECNSLRHKDRRKESVDVRSISSHSSDAPSLQNRRYSSMARIHSMTIEAPITKVINIINAAQESSPVTVAEALDRVLEILRTTELYSPQLASKEDDPHTNDLVGGLMSDGLRRLSGNEYIFSKSALQNMSQSQLGVPVPLNDIPPSIAELLNEEEQWEFNILELEAATHKRPLSYLGLKIFSAFGVCEFLNCSESTLRLWLQVIETNYHSSNSYHNSTHAADVLHATAYFLRKERVKASLDQLDEVAALLAATVHDVDHPGRTNSFLCNAGSELAILYNDTAVLESHHAALAFQLTVRDSKCNIFKNMERTQFRTLRQAIIDMVLATEMTRHFEHVNKFVNSINKPMSAIEETSSNSEGSDCEGQASIRNSPENRLLIKRMLIKCADVANPCRPLELCIEWAGRISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFASLPGLMEHLAENYKYWKNLDEMKCKSLRPPPPP

Target ORF information:

RefSeq Version	XM_009295181.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphodiesterase 8B (pde8b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295181.2

ORF Insert Sequence:

ATGTTACTCC AGGGCTCGGA GATGGTGTCT GGTGCTGAGG ATCGATTGGG GCCGATGCGA 
CTGAGCCATG AACCCATACA GGTGTTACTG GTCTTCGCCA AAGAGGACAG TCAGAGCGAT 
GCCTTCTGGT GGGCGTGCGA GAGGGCCGCT TTCAGGTGCA ACATCGCACG CACACCTGAG 
TCTGCGCTCG AGTGTTTCCT GGACAAACAC CACGAGATCG TCATCATCGA TGGACGACAC 
CCGCGATACT TTGAGGCCGA GGCCATCTGT CGGATGATTC GAGCAACCAA GCTGTCTGAA 
CACACAGTTA TTTTGGCAGT GATGCCCCAA ACTCCGTCAG ATCAGGAGGA ACCATCTGTC 
CTTCCTCTTC TCTCTGCTGG CTTCTCTAGA CGGTACCTGG AGAACAGCAG TGTTTCAGCC 
TGCTATAATG AGTTAATCCA GCTGGAGCAC GGAGAGGTTC GGGCTCAGTT CAAGCTGAGG 
GCTTGTAACG CTGTGTTTAC TGCACTAGAA CAGTGTCAGG AGGCCGTGGA GATCAGCAGT 
GAGGATCATG TCATACAATA TGTAAATCCA GCGTTTGAAA AGATGATGGG TTATCATCGA 
GGAGAACTGA TCGGTAAGGA GCTGACCGAA CTGCCCAGGA GTGACAAGAA CAGCGCCGAC 
CTGCTGGACA CCATCAACAC CTGCATCAAG AAAGGAAAGG AATGGCAGGG GATCTATTTC 
TCAAGGAGGA AGTCAGGGGA CAGTATTCAG CAATATGTCA AAATCACTCC TGTGGTTGGC 
CAGGGAGGAA AAATCCGTCA TTTCGTGTCC ATCAAGAGGC CTTACAGTGA CAACCACAGA 
CAGGTTCACA AGCTGAGCAA AGAAGAGAAA TGCTGTGGTG AACACTCACA ATCAGAATGC 
AACTCCTTGC GTCACAAGGA CCGGAGGAAA GAGTCTGTGG ACGTCAGATC AATCAGCTCT 
CACAGCAGCG ACGCTCCTAG TCTACAGAAC CGCAGATATT CCTCTATGGC CCGAATTCAT 
TCGATGACGA TTGAGGCTCC GATCACTAAG GTGATAAATA TCATTAACGC CGCGCAGGAG 
AGCAGTCCGG TGACGGTAGC GGAAGCTCTG GATCGTGTTC TGGAGATTTT GAGGACCACT 
GAACTCTACT CACCCCAGCT GGCGTCAAAA GAGGACGATC CCCACACCAA TGACCTGGTC 
GGGGGACTCA TGAGTGACGG ACTCAGAAGG CTCTCCGGGA ACGAGTACAT CTTCTCCAAA 
AGTGCTTTAC AAAACATGAG TCAGAGTCAG CTGGGTGTGC CCGTCCCGCT GAATGACATC 
CCGCCGTCCA TCGCAGAGCT GCTGAATGAA GAGGAGCAGT GGGAGTTCAA CATCCTGGAG 
CTGGAGGCAG CCACACACAA GAGGCCACTG TCGTACCTGG GTCTGAAGAT CTTCTCTGCG 
TTTGGCGTGT GCGAGTTTCT GAACTGCTCC GAGTCCACGC TGCGCTTGTG GCTGCAGGTG 
ATCGAGACCA ACTATCACTC CTCAAACTCC TACCATAACT CCACACACGC CGCTGACGTG 
CTGCACGCCA CCGCTTACTT CCTGCGCAAG GAGAGAGTCA AGGCCAGTCT TGACCAGCTA 
GATGAAGTTG CCGCTCTGTT AGCTGCGACA GTTCATGACG TGGATCATCC GGGCCGCACA 
AACTCATTCC TGTGCAACGC AGGCAGTGAA TTGGCCATTC TGTACAACGA TACAGCCGTA 
CTGGAGAGTC ACCATGCTGC TCTCGCCTTC CAGCTCACCG TACGGGACAG CAAGTGCAAC 
ATCTTCAAGA ACATGGAAAG GACTCAGTTT CGGACGCTGC GGCAGGCCAT AATCGACATG 
GTATTGGCAA CAGAAATGAC TCGACACTTC GAACACGTCA ATAAGTTCGT CAACAGCATC 
AACAAACCCA TGAGTGCCAT AGAAGAGACA AGTTCCAATA GTGAAGGCAG TGATTGTGAG 
GGTCAGGCCA GTATTCGTAA CTCTCCTGAG AATCGGCTAC TGATTAAACG AATGCTGATT 
AAATGTGCGG ATGTGGCCAA TCCCTGTCGA CCGCTGGAGC TCTGCATTGA ATGGGCCGGT 
CGCATTTCAG AGGAGTATTT CGCTCAGACT GATGAAGAGA AAAGACAGGG TTTGCCGGTT 
GTCATGCCAG TGTTTGACCG GAACACCTGC AGCATTCCTA AATCCCAGAT CTCCTTCATC 
GACTATTTCA TCACAGACAT GTTTGATGCA TGGGATGCCT TTGCCAGTTT GCCAGGTCTG 
ATGGAGCATT TGGCAGAGAA CTACAAATAC TGGAAGAACT TGGATGAGAT GAAATGTAAA 
AGCCTTCGGC CTCCACCTCC TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde8b ( XM_009295181.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293456.1 Danio rerio phosphodiesterase 8B (pde8b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa46068
Clone ID Related Accession (Same CDS sequence)	XM_009295181.2 , XM_009295181.3
Accession Version	XM_009295181.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295181.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTACTCC AGGGCTCGGA GATGGTGTCT GGTGCTGAGG ATCGATTGGG GCCGATGCGA 
CTGAGCCATG AACCCATACA GGTGTTACTG GTCTTCGCCA AAGAGGACAG TCAGAGCGAT 
GCCTTCTGGT GGGCGTGCGA GAGGGCCGCT TTCAGGTGCA ACATCGCACG CACACCTGAG 
TCTGCGCTCG AGTGTTTCCT GGACAAACAC CACGAGATCG TCATCATCGA TGGACGACAC 
CCGCGATACT TTGAGGCCGA GGCCATCTGT CGGATGATTC GAGCAACCAA GCTGTCTGAA 
CACACAGTTA TTTTGGCAGT GATGCCCCAA ACTCCGTCAG ATCAGGAGGA ACCATCTGTC 
CTTCCTCTTC TCTCTGCTGG CTTCTCTAGA CGGTACCTGG AGAACAGCAG TGTTTCAGCC 
TGCTATAATG AGTTAATCCA GCTGGAGCAC GGAGAGGTTC GGGCTCAGTT CAAGCTGAGG 
GCTTGTAACG CTGTGTTTAC TGCACTAGAA CAGTGTCAGG AGGCCGTGGA GATCAGCAGT 
GAGGATCATG TCATACAATA TGTAAATCCA GCGTTTGAAA AGATGATGGG TTATCATCGA 
GGAGAACTGA TCGGTAAGGA GCTGACCGAA CTGCCCAGGA GTGACAAGAA CAGCGCCGAC 
CTGCTGGACA CCATCAACAC CTGCATCAAG AAAGGAAAGG AATGGCAGGG GATCTATTTC 
TCAAGGAGGA AGTCAGGGGA CAGTATTCAG CAATATGTCA AAATCACTCC TGTGGTTGGC 
CAGGGAGGAA AAATCCGTCA TTTCGTGTCC ATCAAGAGGC CTTACAGTGA CAACCACAGA 
CAGGTTCACA AGCTGAGCAA AGAAGAGAAA TGCTGTGGTG AACACTCACA ATCAGAATGC 
AACTCCTTGC GTCACAAGGA CCGGAGGAAA GAGTCTGTGG ACGTCAGATC AATCAGCTCT 
CACAGCAGCG ACGCTCCTAG TCTACAGAAC CGCAGATATT CCTCTATGGC CCGAATTCAT 
TCGATGACGA TTGAGGCTCC GATCACTAAG GTGATAAATA TCATTAACGC CGCGCAGGAG 
AGCAGTCCGG TGACGGTAGC GGAAGCTCTG GATCGTGTTC TGGAGATTTT GAGGACCACT 
GAACTCTACT CACCCCAGCT GGCGTCAAAA GAGGACGATC CCCACACCAA TGACCTGGTC 
GGGGGACTCA TGAGTGACGG ACTCAGAAGG CTCTCCGGGA ACGAGTACAT CTTCTCCAAA 
AGTGCTTTAC AAAACATGAG TCAGAGTCAG CTGGGTGTGC CCGTCCCGCT GAATGACATC 
CCGCCGTCCA TCGCAGAGCT GCTGAATGAA GAGGAGCAGT GGGAGTTCAA CATCCTGGAG 
CTGGAGGCAG CCACACACAA GAGGCCACTG TCGTACCTGG GTCTGAAGAT CTTCTCTGCG 
TTTGGCGTGT GCGAGTTTCT GAACTGCTCC GAGTCCACGC TGCGCTTGTG GCTGCAGGTG 
ATCGAGACCA ACTATCACTC CTCAAACTCC TACCATAACT CCACACACGC CGCTGACGTG 
CTGCACGCCA CCGCTTACTT CCTGCGCAAG GAGAGAGTCA AGGCCAGTCT TGACCAGCTA 
GATGAAGTTG CCGCTCTGTT AGCTGCGACA GTTCATGACG TGGATCATCC GGGCCGCACA 
AACTCATTCC TGTGCAACGC AGGCAGTGAA TTGGCCATTC TGTACAACGA TACAGCCGTA 
CTGGAGAGTC ACCATGCTGC TCTCGCCTTC CAGCTCACCG TACGGGACAG CAAGTGCAAC 
ATCTTCAAGA ACATGGAAAG GACTCAGTTT CGGACGCTGC GGCAGGCCAT AATCGACATG 
GTATTGGCAA CAGAAATGAC TCGACACTTC GAACACGTCA ATAAGTTCGT CAACAGCATC 
AACAAACCCA TGAGTGCCAT AGAAGAGACA AGTTCCAATA GTGAAGGCAG TGATTGTGAG 
GGTCAGGCCA GTATTCGTAA CTCTCCTGAG AATCGGCTAC TGATTAAACG AATGCTGATT 
AAATGTGCGG ATGTGGCCAA TCCCTGTCGA CCGCTGGAGC TCTGCATTGA ATGGGCCGGT 
CGCATTTCAG AGGAGTATTT CGCTCAGACT GATGAAGAGA AAAGACAGGG TTTGCCGGTT 
GTCATGCCAG TGTTTGACCG GAACACCTGC AGCATTCCTA AATCCCAGAT CTCCTTCATC 
GACTATTTCA TCACAGACAT GTTTGATGCA TGGGATGCCT TTGCCAGTTT GCCAGGTCTG 
ATGGAGCATT TGGCAGAGAA CTACAAATAC TGGAAGAACT TGGATGAGAT GAAATGTAAA 
AGCCTTCGGC CTCCACCTCC TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293456.1
CDS	341..2707
Translation	MLLQGSEMVSGAEDRLGPMRLSHEPIQVLLVFAKEDSQSDAFWWACERAAFRCNIARTPESALECFLDKHHEIVIIDGRHPRYFEAEAICRMIRATKLSEHTVILAVMPQTPSDQEEPSVLPLLSAGFSRRYLENSSVSACYNELIQLEHGEVRAQFKLRACNAVFTALEQCQEAVEISSEDHVIQYVNPAFEKMMGYHRGELIGKELTELPRSDKNSADLLDTINTCIKKGKEWQGIYFSRRKSGDSIQQYVKITPVVGQGGKIRHFVSIKRPYSDNHRQVHKLSKEEKCCGEHSQSECNSLRHKDRRKESVDVRSISSHSSDAPSLQNRRYSSMARIHSMTIEAPITKVINIINAAQESSPVTVAEALDRVLEILRTTELYSPQLASKEDDPHTNDLVGGLMSDGLRRLSGNEYIFSKSALQNMSQSQLGVPVPLNDIPPSIAELLNEEEQWEFNILELEAATHKRPLSYLGLKIFSAFGVCEFLNCSESTLRLWLQVIETNYHSSNSYHNSTHAADVLHATAYFLRKERVKASLDQLDEVAALLAATVHDVDHPGRTNSFLCNAGSELAILYNDTAVLESHHAALAFQLTVRDSKCNIFKNMERTQFRTLRQAIIDMVLATEMTRHFEHVNKFVNSINKPMSAIEETSSNSEGSDCEGQASIRNSPENRLLIKRMLIKCADVANPCRPLELCIEWAGRISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFASLPGLMEHLAENYKYWKNLDEMKCKSLRPPPPP

Target ORF information:

RefSeq Version	XM_009295181.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphodiesterase 8B (pde8b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295181.3

ORF Insert Sequence:

ATGTTACTCC AGGGCTCGGA GATGGTGTCT GGTGCTGAGG ATCGATTGGG GCCGATGCGA 
CTGAGCCATG AACCCATACA GGTGTTACTG GTCTTCGCCA AAGAGGACAG TCAGAGCGAT 
GCCTTCTGGT GGGCGTGCGA GAGGGCCGCT TTCAGGTGCA ACATCGCACG CACACCTGAG 
TCTGCGCTCG AGTGTTTCCT GGACAAACAC CACGAGATCG TCATCATCGA TGGACGACAC 
CCGCGATACT TTGAGGCCGA GGCCATCTGT CGGATGATTC GAGCAACCAA GCTGTCTGAA 
CACACAGTTA TTTTGGCAGT GATGCCCCAA ACTCCGTCAG ATCAGGAGGA ACCATCTGTC 
CTTCCTCTTC TCTCTGCTGG CTTCTCTAGA CGGTACCTGG AGAACAGCAG TGTTTCAGCC 
TGCTATAATG AGTTAATCCA GCTGGAGCAC GGAGAGGTTC GGGCTCAGTT CAAGCTGAGG 
GCTTGTAACG CTGTGTTTAC TGCACTAGAA CAGTGTCAGG AGGCCGTGGA GATCAGCAGT 
GAGGATCATG TCATACAATA TGTAAATCCA GCGTTTGAAA AGATGATGGG TTATCATCGA 
GGAGAACTGA TCGGTAAGGA GCTGACCGAA CTGCCCAGGA GTGACAAGAA CAGCGCCGAC 
CTGCTGGACA CCATCAACAC CTGCATCAAG AAAGGAAAGG AATGGCAGGG GATCTATTTC 
TCAAGGAGGA AGTCAGGGGA CAGTATTCAG CAATATGTCA AAATCACTCC TGTGGTTGGC 
CAGGGAGGAA AAATCCGTCA TTTCGTGTCC ATCAAGAGGC CTTACAGTGA CAACCACAGA 
CAGGTTCACA AGCTGAGCAA AGAAGAGAAA TGCTGTGGTG AACACTCACA ATCAGAATGC 
AACTCCTTGC GTCACAAGGA CCGGAGGAAA GAGTCTGTGG ACGTCAGATC AATCAGCTCT 
CACAGCAGCG ACGCTCCTAG TCTACAGAAC CGCAGATATT CCTCTATGGC CCGAATTCAT 
TCGATGACGA TTGAGGCTCC GATCACTAAG GTGATAAATA TCATTAACGC CGCGCAGGAG 
AGCAGTCCGG TGACGGTAGC GGAAGCTCTG GATCGTGTTC TGGAGATTTT GAGGACCACT 
GAACTCTACT CACCCCAGCT GGCGTCAAAA GAGGACGATC CCCACACCAA TGACCTGGTC 
GGGGGACTCA TGAGTGACGG ACTCAGAAGG CTCTCCGGGA ACGAGTACAT CTTCTCCAAA 
AGTGCTTTAC AAAACATGAG TCAGAGTCAG CTGGGTGTGC CCGTCCCGCT GAATGACATC 
CCGCCGTCCA TCGCAGAGCT GCTGAATGAA GAGGAGCAGT GGGAGTTCAA CATCCTGGAG 
CTGGAGGCAG CCACACACAA GAGGCCACTG TCGTACCTGG GTCTGAAGAT CTTCTCTGCG 
TTTGGCGTGT GCGAGTTTCT GAACTGCTCC GAGTCCACGC TGCGCTTGTG GCTGCAGGTG 
ATCGAGACCA ACTATCACTC CTCAAACTCC TACCATAACT CCACACACGC CGCTGACGTG 
CTGCACGCCA CCGCTTACTT CCTGCGCAAG GAGAGAGTCA AGGCCAGTCT TGACCAGCTA 
GATGAAGTTG CCGCTCTGTT AGCTGCGACA GTTCATGACG TGGATCATCC GGGCCGCACA 
AACTCATTCC TGTGCAACGC AGGCAGTGAA TTGGCCATTC TGTACAACGA TACAGCCGTA 
CTGGAGAGTC ACCATGCTGC TCTCGCCTTC CAGCTCACCG TACGGGACAG CAAGTGCAAC 
ATCTTCAAGA ACATGGAAAG GACTCAGTTT CGGACGCTGC GGCAGGCCAT AATCGACATG 
GTATTGGCAA CAGAAATGAC TCGACACTTC GAACACGTCA ATAAGTTCGT CAACAGCATC 
AACAAACCCA TGAGTGCCAT AGAAGAGACA AGTTCCAATA GTGAAGGCAG TGATTGTGAG 
GGTCAGGCCA GTATTCGTAA CTCTCCTGAG AATCGGCTAC TGATTAAACG AATGCTGATT 
AAATGTGCGG ATGTGGCCAA TCCCTGTCGA CCGCTGGAGC TCTGCATTGA ATGGGCCGGT 
CGCATTTCAG AGGAGTATTT CGCTCAGACT GATGAAGAGA AAAGACAGGG TTTGCCGGTT 
GTCATGCCAG TGTTTGACCG GAACACCTGC AGCATTCCTA AATCCCAGAT CTCCTTCATC 
GACTATTTCA TCACAGACAT GTTTGATGCA TGGGATGCCT TTGCCAGTTT GCCAGGTCTG 
ATGGAGCATT TGGCAGAGAA CTACAAATAC TGGAAGAACT TGGATGAGAT GAAATGTAAA 
AGCCTTCGGC CTCCACCTCC TCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pde8b ( XM_021469002.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324677.1 Danio rerio phosphodiesterase 8B (pde8b), transcript variant X2, mRNA.

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CloneID	ODa25559
Clone ID Related Accession (Same CDS sequence)	XM_021469002.1
Accession Version	XM_021469002.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCGA GCACGAGCAC GCAGCTGCTG TCCAACGGAC CGCGGAGTAA GGTGTCTGGT 
GCTGAGGATC GATTGGGGCC GATGCGACTG AGCCATGAAC CCATACAGGT GTTACTGGTC 
TTCGCCAAAG AGGACAGTCA GAGCGATGCC TTCTGGTGGG CGTGCGAGAG GGCCGCTTTC 
AGGTGCAACA TCGCACGCAC ACCTGAGTCT GCGCTCGAGT GTTTCCTGGA CAAACACCAC 
GAGATCGTCA TCATCGATGG ACGACACCCG CGATACTTTG AGGCCGAGGC CATCTGTCGG 
ATGATTCGAG CAACCAAGCT GTCTGAACAC ACAGTTATTT TGGCAGTGAT GCCCCAAACT 
CCGTCAGATC AGGAGGAACC ATCTGTCCTT CCTCTTCTCT CTGCTGGCTT CTCTAGACGG 
TACCTGGAGA ACAGCAGTGT TTCAGCCTGC TATAATGAGT TAATCCAGCT GGAGCACGGA 
GAGGTTCGGG CTCAGTTCAA GCTGAGGGCT TGTAACGCTG TGTTTACTGC ACTAGAACAG 
TGTCAGGAGG CCGTGGAGAT CAGCAGTGAG GATCATGTCA TACAATATGT AAATCCAGCG 
TTTGAAAAGA TGATGGGTTA TCATCGAGGA GAACTGATCG GTAAGGAGCT GACCGAACTG 
CCCAGGAGTG ACAAGAACAG CGCCGACCTG CTGGACACCA TCAACACCTG CATCAAGAAA 
GGAAAGGAAT GGCAGGGGAT CTATTTCTCA AGGAGGAAGT CAGGGGACAG TATTCAGCAA 
TATGTCAAAA TCACTCCTGT GGTTGGCCAG GGAGGAAAAA TCCGTCATTT CGTGTCCATC 
AAGAGGCCTT ACAGTGACAA CCACAGACAG GTTCACAAGC TGAGCAAAGA AGAGAAATGC 
TGTGGTGAAC ACTCACAATC AGAATGCAAC TCCTTGCGTC ACAAGGACCG GAGGAAAGAG 
TCTGTGGACG TCAGATCAAT CAGCTCTCAC AGCAGCGACG CTCCTAGTCT ACAGAACCGC 
AGATATTCCT CTATGGCCCG AATTCATTCG ATGACGATTG AGGCTCCGAT CACTAAGGTG 
ATAAATATCA TTAACGCCGC GCAGGAGAGC AGTCCGGTGA CGGTAGCGGA AGCTCTGGAT 
CGTGTTCTGG AGATTTTGAG GACCACTGAA CTCTACTCAC CCCAGCTGGC GTCAAAAGAG 
GACGATCCCC ACACCAATGA CCTGGTCGGG GGACTCATGA GTGACGGACT CAGAAGGCTC 
TCCGGGAACG AGTACATCTT CTCCAAAAGT GCTTTACAAA ACATGAGTCA GAGTCAGCTG 
GGTGTGCCCG TCCCGCTGAA TGACATCCCG CCGTCCATCG CAGAGCTGCT GAATGAAGAG 
GAGCAGTGGG AGTTCAACAT CCTGGAGCTG GAGGCAGCCA CACACAAGAG GCCACTGTCG 
TACCTGGGTC TGAAGATCTT CTCTGCGTTT GGCGTGTGCG AGTTTCTGAA CTGCTCCGAG 
TCCACGCTGC GCTTGTGGCT GCAGGTGATC GAGACCAACT ATCACTCCTC AAACTCCTAC 
CATAACTCCA CACACGCCGC TGACGTGCTG CACGCCACCG CTTACTTCCT GCGCAAGGAG 
AGAGTCAAGG CCAGTCTTGA CCAGCTAGAT GAAGTTGCCG CTCTGTTAGC TGCGACAGTT 
CATGACGTGG ATCATCCGGG CCGCACAAAC TCATTCCTGT GCAACGCAGG CAGTGAATTG 
GCCATTCTGT ACAACGATAC AGCCGTACTG GAGAGTCACC ATGCTGCTCT CGCCTTCCAG 
CTCACCGTAC GGGACAGCAA GTGCAACATC TTCAAGAACA TGGAAAGGAC TCAGTTTCGG 
ACGCTGCGGC AGGCCATAAT CGACATGGTA TTGGCAACAG AAATGACTCG ACACTTCGAA 
CACGTCAATA AGTTCGTCAA CAGCATCAAC AAACCCATGA GTGCCATAGA AGAGACAAGT 
TCCAATAGTG AAGGCAGTGA TTGTGAGGGT CAGGCCAGTA TTCGTAACTC TCCTGAGAAT 
CGGCTACTGA TTAAACGAAT GCTGATTAAA TGTGCGGATG TGGCCAATCC CTGTCGACCG 
CTGGAGCTCT GCATTGAATG GGCCGGTCGC ATTTCAGAGG AGTATTTCGC TCAGACTGAT 
GAAGAGAAAA GACAGGGTTT GCCGGTTGTC ATGCCAGTGT TTGACCGGAA CACCTGCAGC 
ATTCCTAAAT CCCAGATCTC CTTCATCGAC TATTTCATCA CAGACATGTT TGATGCATGG 
GATGCCTTTG CCAGTTTGCC AGGTCTGATG GAGCATTTGG CAGAGAACTA CAAATACTGG 
AAGAACTTGG ATGAGATGAA ATGTAAAAGC CTTCGGCCTC CACCTCCTCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324677.1
CDS	219..2612
Translation	MSASTSTQLLSNGPRSKVSGAEDRLGPMRLSHEPIQVLLVFAKEDSQSDAFWWACERAAFRCNIARTPESALECFLDKHHEIVIIDGRHPRYFEAEAICRMIRATKLSEHTVILAVMPQTPSDQEEPSVLPLLSAGFSRRYLENSSVSACYNELIQLEHGEVRAQFKLRACNAVFTALEQCQEAVEISSEDHVIQYVNPAFEKMMGYHRGELIGKELTELPRSDKNSADLLDTINTCIKKGKEWQGIYFSRRKSGDSIQQYVKITPVVGQGGKIRHFVSIKRPYSDNHRQVHKLSKEEKCCGEHSQSECNSLRHKDRRKESVDVRSISSHSSDAPSLQNRRYSSMARIHSMTIEAPITKVINIINAAQESSPVTVAEALDRVLEILRTTELYSPQLASKEDDPHTNDLVGGLMSDGLRRLSGNEYIFSKSALQNMSQSQLGVPVPLNDIPPSIAELLNEEEQWEFNILELEAATHKRPLSYLGLKIFSAFGVCEFLNCSESTLRLWLQVIETNYHSSNSYHNSTHAADVLHATAYFLRKERVKASLDQLDEVAALLAATVHDVDHPGRTNSFLCNAGSELAILYNDTAVLESHHAALAFQLTVRDSKCNIFKNMERTQFRTLRQAIIDMVLATEMTRHFEHVNKFVNSINKPMSAIEETSSNSEGSDCEGQASIRNSPENRLLIKRMLIKCADVANPCRPLELCIEWAGRISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFASLPGLMEHLAENYKYWKNLDEMKCKSLRPPPPP

Target ORF information:

RefSeq Version	XM_021469002.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphodiesterase 8B (pde8b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469002.1

ORF Insert Sequence:

ATGAGCGCGA GCACGAGCAC GCAGCTGCTG TCCAACGGAC CGCGGAGTAA GGTGTCTGGT 
GCTGAGGATC GATTGGGGCC GATGCGACTG AGCCATGAAC CCATACAGGT GTTACTGGTC 
TTCGCCAAAG AGGACAGTCA GAGCGATGCC TTCTGGTGGG CGTGCGAGAG GGCCGCTTTC 
AGGTGCAACA TCGCACGCAC ACCTGAGTCT GCGCTCGAGT GTTTCCTGGA CAAACACCAC 
GAGATCGTCA TCATCGATGG ACGACACCCG CGATACTTTG AGGCCGAGGC CATCTGTCGG 
ATGATTCGAG CAACCAAGCT GTCTGAACAC ACAGTTATTT TGGCAGTGAT GCCCCAAACT 
CCGTCAGATC AGGAGGAACC ATCTGTCCTT CCTCTTCTCT CTGCTGGCTT CTCTAGACGG 
TACCTGGAGA ACAGCAGTGT TTCAGCCTGC TATAATGAGT TAATCCAGCT GGAGCACGGA 
GAGGTTCGGG CTCAGTTCAA GCTGAGGGCT TGTAACGCTG TGTTTACTGC ACTAGAACAG 
TGTCAGGAGG CCGTGGAGAT CAGCAGTGAG GATCATGTCA TACAATATGT AAATCCAGCG 
TTTGAAAAGA TGATGGGTTA TCATCGAGGA GAACTGATCG GTAAGGAGCT GACCGAACTG 
CCCAGGAGTG ACAAGAACAG CGCCGACCTG CTGGACACCA TCAACACCTG CATCAAGAAA 
GGAAAGGAAT GGCAGGGGAT CTATTTCTCA AGGAGGAAGT CAGGGGACAG TATTCAGCAA 
TATGTCAAAA TCACTCCTGT GGTTGGCCAG GGAGGAAAAA TCCGTCATTT CGTGTCCATC 
AAGAGGCCTT ACAGTGACAA CCACAGACAG GTTCACAAGC TGAGCAAAGA AGAGAAATGC 
TGTGGTGAAC ACTCACAATC AGAATGCAAC TCCTTGCGTC ACAAGGACCG GAGGAAAGAG 
TCTGTGGACG TCAGATCAAT CAGCTCTCAC AGCAGCGACG CTCCTAGTCT ACAGAACCGC 
AGATATTCCT CTATGGCCCG AATTCATTCG ATGACGATTG AGGCTCCGAT CACTAAGGTG 
ATAAATATCA TTAACGCCGC GCAGGAGAGC AGTCCGGTGA CGGTAGCGGA AGCTCTGGAT 
CGTGTTCTGG AGATTTTGAG GACCACTGAA CTCTACTCAC CCCAGCTGGC GTCAAAAGAG 
GACGATCCCC ACACCAATGA CCTGGTCGGG GGACTCATGA GTGACGGACT CAGAAGGCTC 
TCCGGGAACG AGTACATCTT CTCCAAAAGT GCTTTACAAA ACATGAGTCA GAGTCAGCTG 
GGTGTGCCCG TCCCGCTGAA TGACATCCCG CCGTCCATCG CAGAGCTGCT GAATGAAGAG 
GAGCAGTGGG AGTTCAACAT CCTGGAGCTG GAGGCAGCCA CACACAAGAG GCCACTGTCG 
TACCTGGGTC TGAAGATCTT CTCTGCGTTT GGCGTGTGCG AGTTTCTGAA CTGCTCCGAG 
TCCACGCTGC GCTTGTGGCT GCAGGTGATC GAGACCAACT ATCACTCCTC AAACTCCTAC 
CATAACTCCA CACACGCCGC TGACGTGCTG CACGCCACCG CTTACTTCCT GCGCAAGGAG 
AGAGTCAAGG CCAGTCTTGA CCAGCTAGAT GAAGTTGCCG CTCTGTTAGC TGCGACAGTT 
CATGACGTGG ATCATCCGGG CCGCACAAAC TCATTCCTGT GCAACGCAGG CAGTGAATTG 
GCCATTCTGT ACAACGATAC AGCCGTACTG GAGAGTCACC ATGCTGCTCT CGCCTTCCAG 
CTCACCGTAC GGGACAGCAA GTGCAACATC TTCAAGAACA TGGAAAGGAC TCAGTTTCGG 
ACGCTGCGGC AGGCCATAAT CGACATGGTA TTGGCAACAG AAATGACTCG ACACTTCGAA 
CACGTCAATA AGTTCGTCAA CAGCATCAAC AAACCCATGA GTGCCATAGA AGAGACAAGT 
TCCAATAGTG AAGGCAGTGA TTGTGAGGGT CAGGCCAGTA TTCGTAACTC TCCTGAGAAT 
CGGCTACTGA TTAAACGAAT GCTGATTAAA TGTGCGGATG TGGCCAATCC CTGTCGACCG 
CTGGAGCTCT GCATTGAATG GGCCGGTCGC ATTTCAGAGG AGTATTTCGC TCAGACTGAT 
GAAGAGAAAA GACAGGGTTT GCCGGTTGTC ATGCCAGTGT TTGACCGGAA CACCTGCAGC 
ATTCCTAAAT CCCAGATCTC CTTCATCGAC TATTTCATCA CAGACATGTT TGATGCATGG 
GATGCCTTTG CCAGTTTGCC AGGTCTGATG GAGCATTTGG CAGAGAACTA CAAATACTGG 
AAGAACTTGG ATGAGATGAA ATGTAAAAGC CTTCGGCCTC CACCTCCTCC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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pde8b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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