glis3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	glis3
Entrez Gene ID	571000
Full Name	GLIS family zinc finger 3
Synonyms	wu:fa55a05,zgc:158249
General protein information	Preferred Names GLIS family zinc finger 3 Names zinc finger protein GLIS3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009305042.2	XP_009303317.1	zinc finger protein GLIS3 isoform X2
XM_017357981.2	XP_017213470.1	zinc finger protein GLIS3 isoform X1
XM_021479193.1	XP_021334868.1	zinc finger protein GLIS3 isoform X3
XM_021479194.1	XP_021334869.1	zinc finger protein GLIS3 isoform X3
XM_017357981.1	XP_017213470.1	zinc finger protein GLIS3 isoform X1
NM_001080607.2	NP_001074076.2	zinc finger protein GLIS3
XM_009305042.3	XP_009303317.1	zinc finger protein GLIS3 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	GLIS3	NP_001035878.1
Canis lupus familiaris (dog)	GLIS3	XP_005615981.1
Mus musculus (house mouse)	Glis3	NP_780668.3
Rattus norvegicus (Norway rat)	Glis3	XP_003749130.1
Bos taurus (cattle)	GLIS3	XP_002689658.2
Pan troglodytes (chimpanzee)	GLIS3	XP_001137126.1
	GLIS3	XP_001083553.2
Xenopus tropicalis (tropical clawed frog)	glis3	XP_002934220.1
Gallus gallus (chicken)	GLIS3	XP_003643037.2
Danio rerio (zebrafish)	glis3	NP_001074076.2

Gene Ontology
Bibliography

Related articles in PubMed

Assignment of Functional Relevance to Genes at Type 2 Diabetes-Associated Loci Through Investigation of ?-Cell Mass Deficits.
O'Hare EA, Yerges-Armstrong LM, Perry JA, Shuldiner AR, Zaghloul NA
Molecular endocrinology (Baltimore, Md.)30(4)429-45(2016 Apr)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Identification and expression analysis of zebrafish polypeptide ?-N-acetylgalactosaminyltransferase Y-subfamily genes during embryonic development.
Nakayama Y, Nakamura N, Kawai T, Kaneda E, Takahashi Y, Miyake A, Itoh N, Kurosaka A
Gene expression patterns : GEP16(1)1-7(2014 Sep)

Systematic discovery of novel ciliary genes through functional genomics in the zebrafish.
Choksi SP, Babu D, Lau D, Yu X, Roy S
Development (Cambridge, England)141(17)3410-9(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following glis3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the glis3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa31355	NM_001080607.2 Latest version!	Danio rerio GLIS family zinc finger 3 (glis3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
ODa147628	XM_021479194.1 Latest version!	Danio rerio GLIS family zinc finger 3 (glis3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
ODa147628	XM_021479193.1 Latest version!	Danio rerio GLIS family zinc finger 3 (glis3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
ODa46056	XM_009305042.3 Latest version!	Danio rerio GLIS family zinc finger 3 (glis3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
ODa54032	XM_017357981.2 Latest version!	Danio rerio GLIS family zinc finger 3 (glis3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
View Old Accession Versions >>
ODa54032	XM_017357981.1	Danio rerio GLIS family zinc finger 3 (glis3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
ODa46056	XM_009305042.2	Danio rerio GLIS family zinc finger 3 (glis3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

glis3 ( NM_001080607.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001074076.2 Danio rerio GLIS family zinc finger 3 (glis3), mRNA.

Price & Availability↑

CloneID	ODa31355
Clone ID Related Accession (Same CDS sequence)	NM_001080607.2
Accession Version	NM_001080607.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-18
Organism	Danio rerio(zebrafish)
Product	zinc finger protein GLIS3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DV591821.1, CU468737.13, CA470456.1, BC129173.1 and CD585814.1. On Sep 27, 2011 this sequence version replaced NM_001080607.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC129173.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACATGA ATGGGAAAGG CTGTAACCTG ATGGTTTCTC CGTCCGGCAT GGCTCAGATA 
TCTCCGCTGA AGGTCCACAC TCCTGCATCT CTCTATCCAG AATCAAACTC AGCTCAGAGT 
CTCCGCCGGC AGGTGCTCAC CAATGGAAAA CTGGGCTTTC CACAGCCATC CCGAGCTCTT 
CATCAACAGG CTCCACAGAT TTCACTCCCA GCATCCTCCT CCTCCAAACA GTCTGCTCGA 
CAATGCTGTC AGGACAGCGG TATGAAAGGT TACAGAAGGG TGTTTGGGTC GTGTGGCAGC 
TCAAACCTGC AGGTCTCCAG CAGTGCTTTT CTGGTCCAGA GTCTCAGTCC TGCTTCTGCA 
TCCGTCACAC ACTCCTCATC ACAAACCTGC CCGCAGAATA CACTCACACT GCCCTTCAAT 
GACGCCAGCA GGTGTTTGCT CTCCAGAGAG TCTCTGGCCT CCACCACCCT TAGTCTGGCT 
GAGACTCAGT CTGTGCGCAG CAGTAAACTG GACTGGCCGT ACGGCTACAG GGTCCTTCCT 
CCACTAGGCC ACCAAACAGC CAATCAGTTG TCAGAATCCA CTGTCCCTGA ATCCTCCCTA 
TCCGAAAGCG TCTCCAACCC TGCATCTCTC CCTCATTACC TCTTCAAGGA AGACGGCAGT 
AGTCCGCGCC TCTCGGCCCG CTCAAAGAAG CGAGCGCTGT CTTTATCCCC ACTATCGGAT 
GGTTTGGGCC TGGACCTGAA CTCCATCATC CGCACCTCGC CTACATCTCT GGTGGCCTAC 
ATCATCGGCT CCAGGATCTC ACCAGCGTCC CACAACACCC CGTCCCCACT GCAGGCGGAC 
GTCTGTGGGC ATCTGCTGGG CATCAGGGGC AGCGGCATCC CTACATCCAG CAAACATAGC 
ATCTCCGTCA GCGAGAGCCT CCGCATGCAG CCGCTGGAGG ACCAGTTCAC CAACCTACTA 
GTGGAGCAGC AGATCCTGCC AGACCCACAA ACTGCAAAGG CCAGTCAGAC CCATACGCTT 
CAGTTTGAGG TGTCCAGGAT TGTCCGGTCC CCCAGCCCAC CCCGGGGGCC ACCACCGCCC 
TACAGCGCCC ACCACCAGCA GCAGCAGCAG CCACGGGTTT TGCAGCTCCA ACGGCCGGCG 
GGGCTTCAGC CGATGCTGGA GGAGGAGGAA GAAGAGGAAG AGTCCTGCGG CGGGACACAC 
TGCTGCTGCT GGCTGCACTG CGGAGCCATC TACAGCCACC GGGACGCGCT GGTCAAACAC 
ATCGAGAAGA TCCACGTGGA CCAGCGCAAA GGAGAGGACT TCACCTGCTT CTGGGCTGGC 
TGCGCGCGCA AACACAAACC CTTCAACGCA CGCTACAAAC TGCTGATCCA CATGAGGGTG 
CACTCGGGGG AGAAGCCCAA CAAGTGCTCG TTCGAGGGCT GTCAGAAGGC GTTTTCCCGC 
CTGGAGAACC TGAAGATCCA CCTGAGGAGC CACACGGGTG AGAAACCCTA CGTGTGTCCA 
CATCCCGGCT GCCTGAAAGC CTTCAGCAAC TCCAGCGACA GAGCCAAACA CCAGCGCACG 
CATGTGGACA CGAAACCGTA CGCGTGTCAG ATCCAGGGCT GTGGAAAGCG CTACACCGAC 
CCCAGCTCAC TGCGGAAACA CGTCAAATCT CACTCGCTGA AGGAGCAGCA GGCCCGGAAG 
AAGTTGAGAC GCAGCTCAGA TCTCAGTCAG GACGGACTCA GCGAGTGTTT GACGGTTCAG 
CCGCTGCAGC ACAGCCTCTC CACACTGGAC CTGATTGAGA GCAAGCACCA CTCACCCACA 
GATGACCTCT ACACCGTCTT CACCCTGAAC CAGCCGAGCC AAACAAACAC CCTGAACAGC 
CCAATGCAGA TCGCAGGCGG TCCACAGACT CATCAAAGTC CTCATCAGAC TCACCAGGCT 
CATCAGATCC ATCAGACTCA GACTCATCTG ACTCACCAGA CCCATCAGGC TCACCAGACT 
CAGACTCATC ACATCCAGCA GATTCATCAG ACTCACCTGA CTCATCAGAT CCAGCAGATT 
CACCAGACTC ATCTCTCTCC ACATGGAGCA CTGCCACCAT TACAGGGAAA CAACAGGTTT 
CTCAGCCCGA ACAGCCACAA AGCTCCGGGA TTCTGCAGTC AGCCCAGGAC ATCTGCTCAG 
GACGGTTTCT CGGCTGTGTT TTCATACGCC GACTCAGACA AACACATCTC CTCCTGCTCC 
GTCATGCAGA CGCCCATGTT TGAGGACAAT CTCAGCTCGT CGCCTCAGAT CCACAGCAAC 
ATCTCCGTGC TGGACTGCCA GAGCGGCCCT CAAGGCTTTA TAGGAGAGCA GGAGCCCTCA 
GTGTCGGAGG ACAATTATCT TCACATCCCG AGATGTCCGA GCCGGATCTC CTGTGTGTTC 
ACTGAAGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001074076.2
CDS	190..2601
Translation	MDMNGKGCNLMVSPSGMAQISPLKVHTPASLYPESNSAQSLRRQVLTNGKLGFPQPSRALHQQAPQISLPASSSSKQSARQCCQDSGMKGYRRVFGSCGSSNLQVSSSAFLVQSLSPASASVTHSSSQTCPQNTLTLPFNDASRCLLSRESLASTTLSLAETQSVRSSKLDWPYGYRVLPPLGHQTANQLSESTVPESSLSESVSNPASLPHYLFKEDGSSPRLSARSKKRALSLSPLSDGLGLDLNSIIRTSPTSLVAYIIGSRISPASHNTPSPLQADVCGHLLGIRGSGIPTSSKHSISVSESLRMQPLEDQFTNLLVEQQILPDPQTAKASQTHTLQFEVSRIVRSPSPPRGPPPPYSAHHQQQQQPRVLQLQRPAGLQPMLEEEEEEEESCGGTHCCCWLHCGAIYSHRDALVKHIEKIHVDQRKGEDFTCFWAGCARKHKPFNARYKLLIHMRVHSGEKPNKCSFEGCQKAFSRLENLKIHLRSHTGEKPYVCPHPGCLKAFSNSSDRAKHQRTHVDTKPYACQIQGCGKRYTDPSSLRKHVKSHSLKEQQARKKLRRSSDLSQDGLSECLTVQPLQHSLSTLDLIESKHHSPTDDLYTVFTLNQPSQTNTLNSPMQIAGGPQTHQSPHQTHQAHQIHQTQTHLTHQTHQAHQTQTHHIQQIHQTHLTHQIQQIHQTHLSPHGALPPLQGNNRFLSPNSHKAPGFCSQPRTSAQDGFSAVFSYADSDKHISSCSVMQTPMFEDNLSSSPQIHSNISVLDCQSGPQGFIGEQEPSVSEDNYLHIPRCPSRISCVFTEG

Target ORF information:

RefSeq Version	NM_001080607.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio GLIS family zinc finger 3 (glis3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001080607.2

ORF Insert Sequence:

ATGGACATGA ATGGGAAAGG CTGTAACCTG ATGGTTTCTC CGTCCGGCAT GGCTCAGATA 
TCTCCGCTGA AGGTCCACAC TCCTGCATCT CTCTATCCAG AATCAAACTC AGCTCAGAGT 
CTCCGCCGGC AGGTGCTCAC CAATGGAAAA CTGGGCTTTC CACAGCCATC CCGAGCTCTT 
CATCAACAGG CTCCACAGAT TTCACTCCCA GCATCCTCCT CCTCCAAACA GTCTGCTCGA 
CAATGCTGTC AGGACAGCGG TATGAAAGGT TACAGAAGGG TGTTTGGGTC GTGTGGCAGC 
TCAAACCTGC AGGTCTCCAG CAGTGCTTTT CTGGTCCAGA GTCTCAGTCC TGCTTCTGCA 
TCCGTCACAC ACTCCTCATC ACAAACCTGC CCGCAGAATA CACTCACACT GCCCTTCAAT 
GACGCCAGCA GGTGTTTGCT CTCCAGAGAG TCTCTGGCCT CCACCACCCT TAGTCTGGCT 
GAGACTCAGT CTGTGCGCAG CAGTAAACTG GACTGGCCGT ACGGCTACAG GGTCCTTCCT 
CCACTAGGCC ACCAAACAGC CAATCAGTTG TCAGAATCCA CTGTCCCTGA ATCCTCCCTA 
TCCGAAAGCG TCTCCAACCC TGCATCTCTC CCTCATTACC TCTTCAAGGA AGACGGCAGT 
AGTCCGCGCC TCTCGGCCCG CTCAAAGAAG CGAGCGCTGT CTTTATCCCC ACTATCGGAT 
GGTTTGGGCC TGGACCTGAA CTCCATCATC CGCACCTCGC CTACATCTCT GGTGGCCTAC 
ATCATCGGCT CCAGGATCTC ACCAGCGTCC CACAACACCC CGTCCCCACT GCAGGCGGAC 
GTCTGTGGGC ATCTGCTGGG CATCAGGGGC AGCGGCATCC CTACATCCAG CAAACATAGC 
ATCTCCGTCA GCGAGAGCCT CCGCATGCAG CCGCTGGAGG ACCAGTTCAC CAACCTACTA 
GTGGAGCAGC AGATCCTGCC AGACCCACAA ACTGCAAAGG CCAGTCAGAC CCATACGCTT 
CAGTTTGAGG TGTCCAGGAT TGTCCGGTCC CCCAGCCCAC CCCGGGGGCC ACCACCGCCC 
TACAGCGCCC ACCACCAGCA GCAGCAGCAG CCACGGGTTT TGCAGCTCCA ACGGCCGGCG 
GGGCTTCAGC CGATGCTGGA GGAGGAGGAA GAAGAGGAAG AGTCCTGCGG CGGGACACAC 
TGCTGCTGCT GGCTGCACTG CGGAGCCATC TACAGCCACC GGGACGCGCT GGTCAAACAC 
ATCGAGAAGA TCCACGTGGA CCAGCGCAAA GGAGAGGACT TCACCTGCTT CTGGGCTGGC 
TGCGCGCGCA AACACAAACC CTTCAACGCA CGCTACAAAC TGCTGATCCA CATGAGGGTG 
CACTCGGGGG AGAAGCCCAA CAAGTGCTCG TTCGAGGGCT GTCAGAAGGC GTTTTCCCGC 
CTGGAGAACC TGAAGATCCA CCTGAGGAGC CACACGGGTG AGAAACCCTA CGTGTGTCCA 
CATCCCGGCT GCCTGAAAGC CTTCAGCAAC TCCAGCGACA GAGCCAAACA CCAGCGCACG 
CATGTGGACA CGAAACCGTA CGCGTGTCAG ATCCAGGGCT GTGGAAAGCG CTACACCGAC 
CCCAGCTCAC TGCGGAAACA CGTCAAATCT CACTCGCTGA AGGAGCAGCA GGCCCGGAAG 
AAGTTGAGAC GCAGCTCAGA TCTCAGTCAG GACGGACTCA GCGAGTGTTT GACGGTTCAG 
CCGCTGCAGC ACAGCCTCTC CACACTGGAC CTGATTGAGA GCAAGCACCA CTCACCCACA 
GATGACCTCT ACACCGTCTT CACCCTGAAC CAGCCGAGCC AAACAAACAC CCTGAACAGC 
CCAATGCAGA TCGCAGGCGG TCCACAGACT CATCAAAGTC CTCATCAGAC TCACCAGGCT 
CATCAGATCC ATCAGACTCA GACTCATCTG ACTCACCAGA CCCATCAGGC TCACCAGACT 
CAGACTCATC ACATCCAGCA GATTCATCAG ACTCACCTGA CTCATCAGAT CCAGCAGATT 
CACCAGACTC ATCTCTCTCC ACATGGAGCA CTGCCACCAT TACAGGGAAA CAACAGGTTT 
CTCAGCCCGA ACAGCCACAA AGCTCCGGGA TTCTGCAGTC AGCCCAGGAC ATCTGCTCAG 
GACGGTTTCT CGGCTGTGTT TTCATACGCC GACTCAGACA AACACATCTC CTCCTGCTCC 
GTCATGCAGA CGCCCATGTT TGAGGACAAT CTCAGCTCGT CGCCTCAGAT CCACAGCAAC 
ATCTCCGTGC TGGACTGCCA GAGCGGCCCT CAAGGCTTTA TAGGAGAGCA GGAGCCCTCA 
GTGTCGGAGG ACAATTATCT TCACATCCCG AGATGTCCGA GCCGGATCTC CTGTGTGTTC 
ACTGAAGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

glis3 ( XM_021479193.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334868.1 Danio rerio GLIS family zinc finger 3 (glis3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa147628
Clone ID Related Accession (Same CDS sequence)	XM_021479193.1 , XM_021479194.1
Accession Version	XM_021479193.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	zinc finger protein GLIS3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATGA ATGGGAAAGG CTGTAACCTG ATGGTTTCTC CGTCCGGCAT GGCTCAGATA 
TCTCCGCTGA AGGTCCACAC TCCTGCATCT CTCTATCCAG AATCAAACTC AGCTCAGAGT 
CTCCGCCGGC AGGTGCTCAC CAATGGAAAA CTGGGCTTTC CACAGCCATC CCGAGCTCTT 
CATCAACAGG CTCCACAGAT TTCACTCCCA GCATCCTCCT CCTCCAAACA GTCTGCTCGA 
CAATGCTGTC AGGACAGCGG TATGAAAGGT TACAGAAGGG TGTTTGGGTC GTGTGGCAGC 
TCAAACCTGC AGGTCTCCAG CAGTGCTTTT CTGGTCCAGA GTCTCAGTCC TGCTTCTGCA 
TCCGTCACAC ACTCCTCATC ACAAACCTGC CCGCAGAATA CACTCACACT GCCCTTCAAT 
GACGCCAGCA GGTGTTTGCT CTCCAGAGAG TCTCTGGCCT CCACCACCCT TAGTCTGGCT 
GAGACTCAGT CTGTGCGCAG CAGTAAACTG GACTGGCCGT ACGGCTACAG GGTCCTTCCT 
CCACTAGGCC ACCAAACAGC CAATCAGTTG TCAGAATCCA CTGTCCCTGA ATCCTCCCTA 
TCCGAAAGCG TCTCCAACCC TGCATCTCTC CCTCATTACC TCTTCAAGGA AGACGGCAGT 
AGTCCGCGCC TCTCGGCCCG CTCAAAGAAG CGAGCGCTGT CTTTATCCCC ACTATCGGAT 
GGTTTGGGCC TGGACCTGAA CTCCATCATC CGCACCTCGC CTACATCTCT GGTGGCCTAC 
ATCATCGGCT CCAGGATCTC ACCAGCGTCC CACAACACCC CGTCCCCACT GCAGGCGGAC 
GTCTGTGGGC ATCTGCTGGG CATCAGGGGC AGCGGCATCC CTACATCCAG CAAACATAGC 
ATCTCCGTCA GCGAGAGCCT CCGCATGCAG CCGCTGGAGG ACCAGTTCAC CAACCTACTA 
GTGGAGCAGC AGATCCTGCC AGACCCACAA ACTGCAAAGG CCAGTCAGAC CCATACGCTT 
CAGTTTGAGG TGTCCAGGAT TGTCCGGTCC CCCAGCCCAC CCCGGGGGCC ACCACCGCCC 
TACAGCGCCC ACCACCAGCA GCAGCAGCAG CCACGGGTTT TGCAGCTCCA ACGGCCGGCG 
GGGCTTCAGC CGATGCTGGA GGAGGAGGAA GAAGAGGAAG AGTCCTGCGG CGGGACACAC 
TGCTGCTGCT GGCTGCACTG CGGAGCCATC TACAGCCACC GGGACGCGCT GGTCAAACAC 
ATCGAGAAGA TCCACGTGGA CCAGCGCAAA GGAGAGGACT TCACCTGCTT CTGGGCTGGC 
TGCGCGCGCA AACACAAACC CTTCAACGCA CGCTACAAAC TGCTGATCCA CATGAGGGTG 
CACTCGGGGG AGAAGCCCAA CAAGTGCTCG TTCGAGGGCT GTCAGAAGGC GTTTTCCCGC 
CTGGAGAACC TGAAGATCCA CCTGAGGAGC CACACGGGTG AGAAACCCTA CGTGTGTCCA 
CATCCCGGCT GCCTGAAAGC CTTCAGCAAC TCCAGCGACA GAGCCAAACA CCAGCGCACG 
CATGTGGACA CGAAACCGTA CGCGTGTCAG ATCCAGGGCT GTGGAAAGCG CTACACCGAC 
CCCAGCTCAC TGCGGAAACA CGTCAAATCT CACTCGCTGA AGGAGCAGCA GGCCCGGAAG 
AAGTTGAGAC GCAGCTCAGA TCTCAGTCAG GACGGACTCA GCGAGTGTTT GACGGTTCAG 
CCGCTGCAGC ACAGCCTCTC CACACTGGAC CTGATTGAGA GCAAGCACCA CTCACCCACA 
GATGACCTCT ACACCGTCTT CACCCTGAAC CAGCCGAGCC AAACAAACAC CCTGAACAGC 
CCAATGCAGA TCGCAGGCGG TCCACAGACT CATCAAAGTC CTCATCAGAC TCACCAGGCT 
CATCAGATCC ATCAGACTCA GACTCATCTG ACTCACCAGA CCCATCAGGC TCACCAGACT 
CAGACTCATC ACATCCAGCA GATTCATCAG ACTCACCTGA CTCATCAGAT CCAGCAGATT 
CACCAGACTC ATCTCTCTCC ACATGGAGCA CTGCCACCAT TACAGGGAAA CAACAGGTTT 
CTCGGCTGTG TTTTCATACG CCGACTCAGA CAAACACATC TCCTCCTGCT CCGTCATGCA 
GACGCCCATG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334868.1
CDS	265..2439
Translation	MDMNGKGCNLMVSPSGMAQISPLKVHTPASLYPESNSAQSLRRQVLTNGKLGFPQPSRALHQQAPQISLPASSSSKQSARQCCQDSGMKGYRRVFGSCGSSNLQVSSSAFLVQSLSPASASVTHSSSQTCPQNTLTLPFNDASRCLLSRESLASTTLSLAETQSVRSSKLDWPYGYRVLPPLGHQTANQLSESTVPESSLSESVSNPASLPHYLFKEDGSSPRLSARSKKRALSLSPLSDGLGLDLNSIIRTSPTSLVAYIIGSRISPASHNTPSPLQADVCGHLLGIRGSGIPTSSKHSISVSESLRMQPLEDQFTNLLVEQQILPDPQTAKASQTHTLQFEVSRIVRSPSPPRGPPPPYSAHHQQQQQPRVLQLQRPAGLQPMLEEEEEEEESCGGTHCCCWLHCGAIYSHRDALVKHIEKIHVDQRKGEDFTCFWAGCARKHKPFNARYKLLIHMRVHSGEKPNKCSFEGCQKAFSRLENLKIHLRSHTGEKPYVCPHPGCLKAFSNSSDRAKHQRTHVDTKPYACQIQGCGKRYTDPSSLRKHVKSHSLKEQQARKKLRRSSDLSQDGLSECLTVQPLQHSLSTLDLIESKHHSPTDDLYTVFTLNQPSQTNTLNSPMQIAGGPQTHQSPHQTHQAHQIHQTQTHLTHQTHQAHQTQTHHIQQIHQTHLTHQIQQIHQTHLSPHGALPPLQGNNRFLGCVFIRRLRQTHLLLLRHADAHV

Target ORF information:

RefSeq Version	XM_021479193.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio GLIS family zinc finger 3 (glis3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479193.1

ORF Insert Sequence:

ATGGACATGA ATGGGAAAGG CTGTAACCTG ATGGTTTCTC CGTCCGGCAT GGCTCAGATA 
TCTCCGCTGA AGGTCCACAC TCCTGCATCT CTCTATCCAG AATCAAACTC AGCTCAGAGT 
CTCCGCCGGC AGGTGCTCAC CAATGGAAAA CTGGGCTTTC CACAGCCATC CCGAGCTCTT 
CATCAACAGG CTCCACAGAT TTCACTCCCA GCATCCTCCT CCTCCAAACA GTCTGCTCGA 
CAATGCTGTC AGGACAGCGG TATGAAAGGT TACAGAAGGG TGTTTGGGTC GTGTGGCAGC 
TCAAACCTGC AGGTCTCCAG CAGTGCTTTT CTGGTCCAGA GTCTCAGTCC TGCTTCTGCA 
TCCGTCACAC ACTCCTCATC ACAAACCTGC CCGCAGAATA CACTCACACT GCCCTTCAAT 
GACGCCAGCA GGTGTTTGCT CTCCAGAGAG TCTCTGGCCT CCACCACCCT TAGTCTGGCT 
GAGACTCAGT CTGTGCGCAG CAGTAAACTG GACTGGCCGT ACGGCTACAG GGTCCTTCCT 
CCACTAGGCC ACCAAACAGC CAATCAGTTG TCAGAATCCA CTGTCCCTGA ATCCTCCCTA 
TCCGAAAGCG TCTCCAACCC TGCATCTCTC CCTCATTACC TCTTCAAGGA AGACGGCAGT 
AGTCCGCGCC TCTCGGCCCG CTCAAAGAAG CGAGCGCTGT CTTTATCCCC ACTATCGGAT 
GGTTTGGGCC TGGACCTGAA CTCCATCATC CGCACCTCGC CTACATCTCT GGTGGCCTAC 
ATCATCGGCT CCAGGATCTC ACCAGCGTCC CACAACACCC CGTCCCCACT GCAGGCGGAC 
GTCTGTGGGC ATCTGCTGGG CATCAGGGGC AGCGGCATCC CTACATCCAG CAAACATAGC 
ATCTCCGTCA GCGAGAGCCT CCGCATGCAG CCGCTGGAGG ACCAGTTCAC CAACCTACTA 
GTGGAGCAGC AGATCCTGCC AGACCCACAA ACTGCAAAGG CCAGTCAGAC CCATACGCTT 
CAGTTTGAGG TGTCCAGGAT TGTCCGGTCC CCCAGCCCAC CCCGGGGGCC ACCACCGCCC 
TACAGCGCCC ACCACCAGCA GCAGCAGCAG CCACGGGTTT TGCAGCTCCA ACGGCCGGCG 
GGGCTTCAGC CGATGCTGGA GGAGGAGGAA GAAGAGGAAG AGTCCTGCGG CGGGACACAC 
TGCTGCTGCT GGCTGCACTG CGGAGCCATC TACAGCCACC GGGACGCGCT GGTCAAACAC 
ATCGAGAAGA TCCACGTGGA CCAGCGCAAA GGAGAGGACT TCACCTGCTT CTGGGCTGGC 
TGCGCGCGCA AACACAAACC CTTCAACGCA CGCTACAAAC TGCTGATCCA CATGAGGGTG 
CACTCGGGGG AGAAGCCCAA CAAGTGCTCG TTCGAGGGCT GTCAGAAGGC GTTTTCCCGC 
CTGGAGAACC TGAAGATCCA CCTGAGGAGC CACACGGGTG AGAAACCCTA CGTGTGTCCA 
CATCCCGGCT GCCTGAAAGC CTTCAGCAAC TCCAGCGACA GAGCCAAACA CCAGCGCACG 
CATGTGGACA CGAAACCGTA CGCGTGTCAG ATCCAGGGCT GTGGAAAGCG CTACACCGAC 
CCCAGCTCAC TGCGGAAACA CGTCAAATCT CACTCGCTGA AGGAGCAGCA GGCCCGGAAG 
AAGTTGAGAC GCAGCTCAGA TCTCAGTCAG GACGGACTCA GCGAGTGTTT GACGGTTCAG 
CCGCTGCAGC ACAGCCTCTC CACACTGGAC CTGATTGAGA GCAAGCACCA CTCACCCACA 
GATGACCTCT ACACCGTCTT CACCCTGAAC CAGCCGAGCC AAACAAACAC CCTGAACAGC 
CCAATGCAGA TCGCAGGCGG TCCACAGACT CATCAAAGTC CTCATCAGAC TCACCAGGCT 
CATCAGATCC ATCAGACTCA GACTCATCTG ACTCACCAGA CCCATCAGGC TCACCAGACT 
CAGACTCATC ACATCCAGCA GATTCATCAG ACTCACCTGA CTCATCAGAT CCAGCAGATT 
CACCAGACTC ATCTCTCTCC ACATGGAGCA CTGCCACCAT TACAGGGAAA CAACAGGTTT 
CTCGGCTGTG TTTTCATACG CCGACTCAGA CAAACACATC TCCTCCTGCT CCGTCATGCA 
GACGCCCATG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

glis3 ( XM_021479194.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334869.1 Danio rerio GLIS family zinc finger 3 (glis3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa147628
Clone ID Related Accession (Same CDS sequence)	XM_021479193.1 , XM_021479194.1
Accession Version	XM_021479194.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	zinc finger protein GLIS3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATGA ATGGGAAAGG CTGTAACCTG ATGGTTTCTC CGTCCGGCAT GGCTCAGATA 
TCTCCGCTGA AGGTCCACAC TCCTGCATCT CTCTATCCAG AATCAAACTC AGCTCAGAGT 
CTCCGCCGGC AGGTGCTCAC CAATGGAAAA CTGGGCTTTC CACAGCCATC CCGAGCTCTT 
CATCAACAGG CTCCACAGAT TTCACTCCCA GCATCCTCCT CCTCCAAACA GTCTGCTCGA 
CAATGCTGTC AGGACAGCGG TATGAAAGGT TACAGAAGGG TGTTTGGGTC GTGTGGCAGC 
TCAAACCTGC AGGTCTCCAG CAGTGCTTTT CTGGTCCAGA GTCTCAGTCC TGCTTCTGCA 
TCCGTCACAC ACTCCTCATC ACAAACCTGC CCGCAGAATA CACTCACACT GCCCTTCAAT 
GACGCCAGCA GGTGTTTGCT CTCCAGAGAG TCTCTGGCCT CCACCACCCT TAGTCTGGCT 
GAGACTCAGT CTGTGCGCAG CAGTAAACTG GACTGGCCGT ACGGCTACAG GGTCCTTCCT 
CCACTAGGCC ACCAAACAGC CAATCAGTTG TCAGAATCCA CTGTCCCTGA ATCCTCCCTA 
TCCGAAAGCG TCTCCAACCC TGCATCTCTC CCTCATTACC TCTTCAAGGA AGACGGCAGT 
AGTCCGCGCC TCTCGGCCCG CTCAAAGAAG CGAGCGCTGT CTTTATCCCC ACTATCGGAT 
GGTTTGGGCC TGGACCTGAA CTCCATCATC CGCACCTCGC CTACATCTCT GGTGGCCTAC 
ATCATCGGCT CCAGGATCTC ACCAGCGTCC CACAACACCC CGTCCCCACT GCAGGCGGAC 
GTCTGTGGGC ATCTGCTGGG CATCAGGGGC AGCGGCATCC CTACATCCAG CAAACATAGC 
ATCTCCGTCA GCGAGAGCCT CCGCATGCAG CCGCTGGAGG ACCAGTTCAC CAACCTACTA 
GTGGAGCAGC AGATCCTGCC AGACCCACAA ACTGCAAAGG CCAGTCAGAC CCATACGCTT 
CAGTTTGAGG TGTCCAGGAT TGTCCGGTCC CCCAGCCCAC CCCGGGGGCC ACCACCGCCC 
TACAGCGCCC ACCACCAGCA GCAGCAGCAG CCACGGGTTT TGCAGCTCCA ACGGCCGGCG 
GGGCTTCAGC CGATGCTGGA GGAGGAGGAA GAAGAGGAAG AGTCCTGCGG CGGGACACAC 
TGCTGCTGCT GGCTGCACTG CGGAGCCATC TACAGCCACC GGGACGCGCT GGTCAAACAC 
ATCGAGAAGA TCCACGTGGA CCAGCGCAAA GGAGAGGACT TCACCTGCTT CTGGGCTGGC 
TGCGCGCGCA AACACAAACC CTTCAACGCA CGCTACAAAC TGCTGATCCA CATGAGGGTG 
CACTCGGGGG AGAAGCCCAA CAAGTGCTCG TTCGAGGGCT GTCAGAAGGC GTTTTCCCGC 
CTGGAGAACC TGAAGATCCA CCTGAGGAGC CACACGGGTG AGAAACCCTA CGTGTGTCCA 
CATCCCGGCT GCCTGAAAGC CTTCAGCAAC TCCAGCGACA GAGCCAAACA CCAGCGCACG 
CATGTGGACA CGAAACCGTA CGCGTGTCAG ATCCAGGGCT GTGGAAAGCG CTACACCGAC 
CCCAGCTCAC TGCGGAAACA CGTCAAATCT CACTCGCTGA AGGAGCAGCA GGCCCGGAAG 
AAGTTGAGAC GCAGCTCAGA TCTCAGTCAG GACGGACTCA GCGAGTGTTT GACGGTTCAG 
CCGCTGCAGC ACAGCCTCTC CACACTGGAC CTGATTGAGA GCAAGCACCA CTCACCCACA 
GATGACCTCT ACACCGTCTT CACCCTGAAC CAGCCGAGCC AAACAAACAC CCTGAACAGC 
CCAATGCAGA TCGCAGGCGG TCCACAGACT CATCAAAGTC CTCATCAGAC TCACCAGGCT 
CATCAGATCC ATCAGACTCA GACTCATCTG ACTCACCAGA CCCATCAGGC TCACCAGACT 
CAGACTCATC ACATCCAGCA GATTCATCAG ACTCACCTGA CTCATCAGAT CCAGCAGATT 
CACCAGACTC ATCTCTCTCC ACATGGAGCA CTGCCACCAT TACAGGGAAA CAACAGGTTT 
CTCGGCTGTG TTTTCATACG CCGACTCAGA CAAACACATC TCCTCCTGCT CCGTCATGCA 
GACGCCCATG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334869.1
CDS	266..2440
Translation	MDMNGKGCNLMVSPSGMAQISPLKVHTPASLYPESNSAQSLRRQVLTNGKLGFPQPSRALHQQAPQISLPASSSSKQSARQCCQDSGMKGYRRVFGSCGSSNLQVSSSAFLVQSLSPASASVTHSSSQTCPQNTLTLPFNDASRCLLSRESLASTTLSLAETQSVRSSKLDWPYGYRVLPPLGHQTANQLSESTVPESSLSESVSNPASLPHYLFKEDGSSPRLSARSKKRALSLSPLSDGLGLDLNSIIRTSPTSLVAYIIGSRISPASHNTPSPLQADVCGHLLGIRGSGIPTSSKHSISVSESLRMQPLEDQFTNLLVEQQILPDPQTAKASQTHTLQFEVSRIVRSPSPPRGPPPPYSAHHQQQQQPRVLQLQRPAGLQPMLEEEEEEEESCGGTHCCCWLHCGAIYSHRDALVKHIEKIHVDQRKGEDFTCFWAGCARKHKPFNARYKLLIHMRVHSGEKPNKCSFEGCQKAFSRLENLKIHLRSHTGEKPYVCPHPGCLKAFSNSSDRAKHQRTHVDTKPYACQIQGCGKRYTDPSSLRKHVKSHSLKEQQARKKLRRSSDLSQDGLSECLTVQPLQHSLSTLDLIESKHHSPTDDLYTVFTLNQPSQTNTLNSPMQIAGGPQTHQSPHQTHQAHQIHQTQTHLTHQTHQAHQTQTHHIQQIHQTHLTHQIQQIHQTHLSPHGALPPLQGNNRFLGCVFIRRLRQTHLLLLRHADAHV

Target ORF information:

RefSeq Version	XM_021479194.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio GLIS family zinc finger 3 (glis3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479194.1

ORF Insert Sequence:

ATGGACATGA ATGGGAAAGG CTGTAACCTG ATGGTTTCTC CGTCCGGCAT GGCTCAGATA 
TCTCCGCTGA AGGTCCACAC TCCTGCATCT CTCTATCCAG AATCAAACTC AGCTCAGAGT 
CTCCGCCGGC AGGTGCTCAC CAATGGAAAA CTGGGCTTTC CACAGCCATC CCGAGCTCTT 
CATCAACAGG CTCCACAGAT TTCACTCCCA GCATCCTCCT CCTCCAAACA GTCTGCTCGA 
CAATGCTGTC AGGACAGCGG TATGAAAGGT TACAGAAGGG TGTTTGGGTC GTGTGGCAGC 
TCAAACCTGC AGGTCTCCAG CAGTGCTTTT CTGGTCCAGA GTCTCAGTCC TGCTTCTGCA 
TCCGTCACAC ACTCCTCATC ACAAACCTGC CCGCAGAATA CACTCACACT GCCCTTCAAT 
GACGCCAGCA GGTGTTTGCT CTCCAGAGAG TCTCTGGCCT CCACCACCCT TAGTCTGGCT 
GAGACTCAGT CTGTGCGCAG CAGTAAACTG GACTGGCCGT ACGGCTACAG GGTCCTTCCT 
CCACTAGGCC ACCAAACAGC CAATCAGTTG TCAGAATCCA CTGTCCCTGA ATCCTCCCTA 
TCCGAAAGCG TCTCCAACCC TGCATCTCTC CCTCATTACC TCTTCAAGGA AGACGGCAGT 
AGTCCGCGCC TCTCGGCCCG CTCAAAGAAG CGAGCGCTGT CTTTATCCCC ACTATCGGAT 
GGTTTGGGCC TGGACCTGAA CTCCATCATC CGCACCTCGC CTACATCTCT GGTGGCCTAC 
ATCATCGGCT CCAGGATCTC ACCAGCGTCC CACAACACCC CGTCCCCACT GCAGGCGGAC 
GTCTGTGGGC ATCTGCTGGG CATCAGGGGC AGCGGCATCC CTACATCCAG CAAACATAGC 
ATCTCCGTCA GCGAGAGCCT CCGCATGCAG CCGCTGGAGG ACCAGTTCAC CAACCTACTA 
GTGGAGCAGC AGATCCTGCC AGACCCACAA ACTGCAAAGG CCAGTCAGAC CCATACGCTT 
CAGTTTGAGG TGTCCAGGAT TGTCCGGTCC CCCAGCCCAC CCCGGGGGCC ACCACCGCCC 
TACAGCGCCC ACCACCAGCA GCAGCAGCAG CCACGGGTTT TGCAGCTCCA ACGGCCGGCG 
GGGCTTCAGC CGATGCTGGA GGAGGAGGAA GAAGAGGAAG AGTCCTGCGG CGGGACACAC 
TGCTGCTGCT GGCTGCACTG CGGAGCCATC TACAGCCACC GGGACGCGCT GGTCAAACAC 
ATCGAGAAGA TCCACGTGGA CCAGCGCAAA GGAGAGGACT TCACCTGCTT CTGGGCTGGC 
TGCGCGCGCA AACACAAACC CTTCAACGCA CGCTACAAAC TGCTGATCCA CATGAGGGTG 
CACTCGGGGG AGAAGCCCAA CAAGTGCTCG TTCGAGGGCT GTCAGAAGGC GTTTTCCCGC 
CTGGAGAACC TGAAGATCCA CCTGAGGAGC CACACGGGTG AGAAACCCTA CGTGTGTCCA 
CATCCCGGCT GCCTGAAAGC CTTCAGCAAC TCCAGCGACA GAGCCAAACA CCAGCGCACG 
CATGTGGACA CGAAACCGTA CGCGTGTCAG ATCCAGGGCT GTGGAAAGCG CTACACCGAC 
CCCAGCTCAC TGCGGAAACA CGTCAAATCT CACTCGCTGA AGGAGCAGCA GGCCCGGAAG 
AAGTTGAGAC GCAGCTCAGA TCTCAGTCAG GACGGACTCA GCGAGTGTTT GACGGTTCAG 
CCGCTGCAGC ACAGCCTCTC CACACTGGAC CTGATTGAGA GCAAGCACCA CTCACCCACA 
GATGACCTCT ACACCGTCTT CACCCTGAAC CAGCCGAGCC AAACAAACAC CCTGAACAGC 
CCAATGCAGA TCGCAGGCGG TCCACAGACT CATCAAAGTC CTCATCAGAC TCACCAGGCT 
CATCAGATCC ATCAGACTCA GACTCATCTG ACTCACCAGA CCCATCAGGC TCACCAGACT 
CAGACTCATC ACATCCAGCA GATTCATCAG ACTCACCTGA CTCATCAGAT CCAGCAGATT 
CACCAGACTC ATCTCTCTCC ACATGGAGCA CTGCCACCAT TACAGGGAAA CAACAGGTTT 
CTCGGCTGTG TTTTCATACG CCGACTCAGA CAAACACATC TCCTCCTGCT CCGTCATGCA 
GACGCCCATG TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

glis3 ( XM_009305042.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303317.1 Danio rerio GLIS family zinc finger 3 (glis3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa46056
Clone ID Related Accession (Same CDS sequence)	XM_009305042.2 , XM_009305042.3
Accession Version	XM_009305042.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	zinc finger protein GLIS3 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305042.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATGA ATGGGAAAGG CTGTAACCTG ATGGTTTCTC CGTCCGGCAT GGCTCAGATA 
TCTCCGCTGA AGGTCCACAC TCCTGCATCT CTCTATCCAG AATCAAACTC AGCTCAGAGT 
CTCCGCCGGC AGGTGCTCAC CAATGGAAAA CTGGGCTTTC CACAGCCATC CCGAGCTCTT 
CATCAACAGG CTCCACAGAT TTCACTCCCA GCATCCTCCT CCTCCAAACA GTCTGCTCGA 
CAATGCTGTC AGGACAGCGG TATGAAAGGT TACAGAAGGG TGTTTGGGTC GTGTGGCAGC 
TCAAACCTGC AGGTCTCCAG CAGTGCTTTT CTGGTCCAGA GTCTCAGTCC TGCTTCTGCA 
TCCGTCACAC ACTCCTCATC ACAAACCTGC CCGCAGAATA CACTCACACT GCCCTTCAAT 
GACGCCAGGT GTTTGCTCTC CAGAGAGTCT CTGGCCTCCA CCACCCTTAG TCTGGCTGAG 
ACTCAGTCTG TGCGCAGCAG TAAACTGGAC TGGCCGTACG GCTACAGGGT CCTTCCTCCA 
CTAGGCCACC AAACAGCCAA TCAGTTGTCA GAATCCACTG TCCCTGAATC CTCCCTATCC 
GAAAGCGTCT CCAACCCTGC ATCTCTCCCT CATTACCTCT TCAAGGAAGA CGGCAGTAGT 
CCGCGCCTCT CGGCCCGCTC AAAGAAGCGA GCGCTGTCTT TATCCCCACT ATCGGATGGT 
TTGGGCCTGG ACCTGAACTC CATCATCCGC ACCTCGCCTA CATCTCTGGT GGCCTACATC 
ATCGGCTCCA GGATCTCACC AGCGTCCCAC AACACCCCGT CCCCACTGCA GGCGGACGTC 
TGTGGGCATC TGCTGGGCAT CAGGGGCAGC GGCATCCCTA CATCCAGCAA ACATAGCATC 
TCCGTCAGCG AGAGCCTCCG CATGCAGCCG CTGGAGGACC AGTTCACCAA CCTACTAGTG 
GAGCAGCAGA TCCTGCCAGA CCCACAAACT GCAAAGGCCA GTCAGACCCA TACGCTTCAG 
TTTGAGGTGT CCAGGATTGT CCGGTCCCCC AGCCCACCCC GGGGGCCACC ACCGCCCTAC 
AGCGCCCACC ACCAGCAGCA GCAGCAGCCA CGGGTTTTGC AGCTCCAACG GCCGGCGGGG 
CTTCAGCCGA TGCTGGAGGA GGAGGAAGAA GAGGAAGAGT CCTGCGGCGG GACACACTGC 
TGCTGCTGGC TGCACTGCGG AGCCATCTAC AGCCACCGGG ACGCGCTGGT CAAACACATC 
GAGAAGATCC ACGTGGACCA GCGCAAAGGA GAGGACTTCA CCTGCTTCTG GGCTGGCTGC 
GCGCGCAAAC ACAAACCCTT CAACGCACGC TACAAACTGC TGATCCACAT GAGGGTGCAC 
TCGGGGGAGA AGCCCAACAA GTGCTCGTTC GAGGGCTGTC AGAAGGCGTT TTCCCGCCTG 
GAGAACCTGA AGATCCACCT GAGGAGCCAC ACGGGTGAGA AACCCTACGT GTGTCCACAT 
CCCGGCTGCC TGAAAGCCTT CAGCAACTCC AGCGACAGAG CCAAACACCA GCGCACGCAT 
GTGGACACGA AACCGTACGC GTGTCAGATC CAGGGCTGTG GAAAGCGCTA CACCGACCCC 
AGCTCACTGC GGAAACACGT CAAATCTCAC TCGCTGAAGG AGCAGCAGGC CCGGAAGAAG 
TTGAGACGCA GCTCAGATCT CAGTCAGGAC GGACTCAGCG AGTGTTTGAC GGTTCAGCCG 
CTGCAGCACA GCCTCTCCAC ACTGGACCTG ATTGAGAGCA AGCACCACTC ACCCACAGAT 
GACCTCTACA CCGTCTTCAC CCTGAACCAG CCGAGCCAAA CAAACACCCT GAACAGCCCA 
ATGCAGATCG CAGGCGGTCC ACAGACTCAT CAAAGTCCTC ATCAGACTCA CCAGGCTCAT 
CAGATCCATC AGACTCAGAC TCATCTGACT CACCAGACCC ATCAGGCTCA CCAGACTCAG 
ACTCATCACA TCCAGCAGAT TCATCAGACT CACCTGACTC ATCAGATCCA GCAGATTCAC 
CAGACTCATC TCTCTCCACA TGGAGCACTG CCACCATTAC AGGGAAACAA CAGGTTTCTC 
AGCCCGAACA GCCACAAAGC TCCGGGATTC TGCAGTCAGC CCAGGACATC TGCTCAGGAC 
GGTTTCTCGG CTGTGTTTTC ATACGCCGAC TCAGACAAAC ACATCTCCTC CTGCTCCGTC 
ATGCAGACGC CCATGTTTGA GGACAATCTC AGCTCGTCGC CTCAGATCCA CAGCAACATC 
TCCGCAGTGC TGGACTGCCA GAGCGGCCCT CAAGGCTTTA TAGGAGAGCA GGAGCCCTCA 
GTGTCGGAGG ACAATTATCT TCACATCCCG AGATGTCCGA GCCGGATCTC CTGTGTGTTC 
ACTGAAGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303317.1
CDS	249..2660
Translation	MDMNGKGCNLMVSPSGMAQISPLKVHTPASLYPESNSAQSLRRQVLTNGKLGFPQPSRALHQQAPQISLPASSSSKQSARQCCQDSGMKGYRRVFGSCGSSNLQVSSSAFLVQSLSPASASVTHSSSQTCPQNTLTLPFNDARCLLSRESLASTTLSLAETQSVRSSKLDWPYGYRVLPPLGHQTANQLSESTVPESSLSESVSNPASLPHYLFKEDGSSPRLSARSKKRALSLSPLSDGLGLDLNSIIRTSPTSLVAYIIGSRISPASHNTPSPLQADVCGHLLGIRGSGIPTSSKHSISVSESLRMQPLEDQFTNLLVEQQILPDPQTAKASQTHTLQFEVSRIVRSPSPPRGPPPPYSAHHQQQQQPRVLQLQRPAGLQPMLEEEEEEEESCGGTHCCCWLHCGAIYSHRDALVKHIEKIHVDQRKGEDFTCFWAGCARKHKPFNARYKLLIHMRVHSGEKPNKCSFEGCQKAFSRLENLKIHLRSHTGEKPYVCPHPGCLKAFSNSSDRAKHQRTHVDTKPYACQIQGCGKRYTDPSSLRKHVKSHSLKEQQARKKLRRSSDLSQDGLSECLTVQPLQHSLSTLDLIESKHHSPTDDLYTVFTLNQPSQTNTLNSPMQIAGGPQTHQSPHQTHQAHQIHQTQTHLTHQTHQAHQTQTHHIQQIHQTHLTHQIQQIHQTHLSPHGALPPLQGNNRFLSPNSHKAPGFCSQPRTSAQDGFSAVFSYADSDKHISSCSVMQTPMFEDNLSSSPQIHSNISAVLDCQSGPQGFIGEQEPSVSEDNYLHIPRCPSRISCVFTEG

Target ORF information:

RefSeq Version	XM_009305042.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio GLIS family zinc finger 3 (glis3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305042.2

ORF Insert Sequence:

ATGGACATGA ATGGGAAAGG CTGTAACCTG ATGGTTTCTC CGTCCGGCAT GGCTCAGATA 
TCTCCGCTGA AGGTCCACAC TCCTGCATCT CTCTATCCAG AATCAAACTC AGCTCAGAGT 
CTCCGCCGGC AGGTGCTCAC CAATGGAAAA CTGGGCTTTC CACAGCCATC CCGAGCTCTT 
CATCAACAGG CTCCACAGAT TTCACTCCCA GCATCCTCCT CCTCCAAACA GTCTGCTCGA 
CAATGCTGTC AGGACAGCGG TATGAAAGGT TACAGAAGGG TGTTTGGGTC GTGTGGCAGC 
TCAAACCTGC AGGTCTCCAG CAGTGCTTTT CTGGTCCAGA GTCTCAGTCC TGCTTCTGCA 
TCCGTCACAC ACTCCTCATC ACAAACCTGC CCGCAGAATA CACTCACACT GCCCTTCAAT 
GACGCCAGGT GTTTGCTCTC CAGAGAGTCT CTGGCCTCCA CCACCCTTAG TCTGGCTGAG 
ACTCAGTCTG TGCGCAGCAG TAAACTGGAC TGGCCGTACG GCTACAGGGT CCTTCCTCCA 
CTAGGCCACC AAACAGCCAA TCAGTTGTCA GAATCCACTG TCCCTGAATC CTCCCTATCC 
GAAAGCGTCT CCAACCCTGC ATCTCTCCCT CATTACCTCT TCAAGGAAGA CGGCAGTAGT 
CCGCGCCTCT CGGCCCGCTC AAAGAAGCGA GCGCTGTCTT TATCCCCACT ATCGGATGGT 
TTGGGCCTGG ACCTGAACTC CATCATCCGC ACCTCGCCTA CATCTCTGGT GGCCTACATC 
ATCGGCTCCA GGATCTCACC AGCGTCCCAC AACACCCCGT CCCCACTGCA GGCGGACGTC 
TGTGGGCATC TGCTGGGCAT CAGGGGCAGC GGCATCCCTA CATCCAGCAA ACATAGCATC 
TCCGTCAGCG AGAGCCTCCG CATGCAGCCG CTGGAGGACC AGTTCACCAA CCTACTAGTG 
GAGCAGCAGA TCCTGCCAGA CCCACAAACT GCAAAGGCCA GTCAGACCCA TACGCTTCAG 
TTTGAGGTGT CCAGGATTGT CCGGTCCCCC AGCCCACCCC GGGGGCCACC ACCGCCCTAC 
AGCGCCCACC ACCAGCAGCA GCAGCAGCCA CGGGTTTTGC AGCTCCAACG GCCGGCGGGG 
CTTCAGCCGA TGCTGGAGGA GGAGGAAGAA GAGGAAGAGT CCTGCGGCGG GACACACTGC 
TGCTGCTGGC TGCACTGCGG AGCCATCTAC AGCCACCGGG ACGCGCTGGT CAAACACATC 
GAGAAGATCC ACGTGGACCA GCGCAAAGGA GAGGACTTCA CCTGCTTCTG GGCTGGCTGC 
GCGCGCAAAC ACAAACCCTT CAACGCACGC TACAAACTGC TGATCCACAT GAGGGTGCAC 
TCGGGGGAGA AGCCCAACAA GTGCTCGTTC GAGGGCTGTC AGAAGGCGTT TTCCCGCCTG 
GAGAACCTGA AGATCCACCT GAGGAGCCAC ACGGGTGAGA AACCCTACGT GTGTCCACAT 
CCCGGCTGCC TGAAAGCCTT CAGCAACTCC AGCGACAGAG CCAAACACCA GCGCACGCAT 
GTGGACACGA AACCGTACGC GTGTCAGATC CAGGGCTGTG GAAAGCGCTA CACCGACCCC 
AGCTCACTGC GGAAACACGT CAAATCTCAC TCGCTGAAGG AGCAGCAGGC CCGGAAGAAG 
TTGAGACGCA GCTCAGATCT CAGTCAGGAC GGACTCAGCG AGTGTTTGAC GGTTCAGCCG 
CTGCAGCACA GCCTCTCCAC ACTGGACCTG ATTGAGAGCA AGCACCACTC ACCCACAGAT 
GACCTCTACA CCGTCTTCAC CCTGAACCAG CCGAGCCAAA CAAACACCCT GAACAGCCCA 
ATGCAGATCG CAGGCGGTCC ACAGACTCAT CAAAGTCCTC ATCAGACTCA CCAGGCTCAT 
CAGATCCATC AGACTCAGAC TCATCTGACT CACCAGACCC ATCAGGCTCA CCAGACTCAG 
ACTCATCACA TCCAGCAGAT TCATCAGACT CACCTGACTC ATCAGATCCA GCAGATTCAC 
CAGACTCATC TCTCTCCACA TGGAGCACTG CCACCATTAC AGGGAAACAA CAGGTTTCTC 
AGCCCGAACA GCCACAAAGC TCCGGGATTC TGCAGTCAGC CCAGGACATC TGCTCAGGAC 
GGTTTCTCGG CTGTGTTTTC ATACGCCGAC TCAGACAAAC ACATCTCCTC CTGCTCCGTC 
ATGCAGACGC CCATGTTTGA GGACAATCTC AGCTCGTCGC CTCAGATCCA CAGCAACATC 
TCCGCAGTGC TGGACTGCCA GAGCGGCCCT CAAGGCTTTA TAGGAGAGCA GGAGCCCTCA 
GTGTCGGAGG ACAATTATCT TCACATCCCG AGATGTCCGA GCCGGATCTC CTGTGTGTTC 
ACTGAAGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

glis3 ( XM_009305042.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303317.1 Danio rerio GLIS family zinc finger 3 (glis3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa46056
Clone ID Related Accession (Same CDS sequence)	XM_009305042.2 , XM_009305042.3
Accession Version	XM_009305042.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2412bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	zinc finger protein GLIS3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305042.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATGA ATGGGAAAGG CTGTAACCTG ATGGTTTCTC CGTCCGGCAT GGCTCAGATA 
TCTCCGCTGA AGGTCCACAC TCCTGCATCT CTCTATCCAG AATCAAACTC AGCTCAGAGT 
CTCCGCCGGC AGGTGCTCAC CAATGGAAAA CTGGGCTTTC CACAGCCATC CCGAGCTCTT 
CATCAACAGG CTCCACAGAT TTCACTCCCA GCATCCTCCT CCTCCAAACA GTCTGCTCGA 
CAATGCTGTC AGGACAGCGG TATGAAAGGT TACAGAAGGG TGTTTGGGTC GTGTGGCAGC 
TCAAACCTGC AGGTCTCCAG CAGTGCTTTT CTGGTCCAGA GTCTCAGTCC TGCTTCTGCA 
TCCGTCACAC ACTCCTCATC ACAAACCTGC CCGCAGAATA CACTCACACT GCCCTTCAAT 
GACGCCAGGT GTTTGCTCTC CAGAGAGTCT CTGGCCTCCA CCACCCTTAG TCTGGCTGAG 
ACTCAGTCTG TGCGCAGCAG TAAACTGGAC TGGCCGTACG GCTACAGGGT CCTTCCTCCA 
CTAGGCCACC AAACAGCCAA TCAGTTGTCA GAATCCACTG TCCCTGAATC CTCCCTATCC 
GAAAGCGTCT CCAACCCTGC ATCTCTCCCT CATTACCTCT TCAAGGAAGA CGGCAGTAGT 
CCGCGCCTCT CGGCCCGCTC AAAGAAGCGA GCGCTGTCTT TATCCCCACT ATCGGATGGT 
TTGGGCCTGG ACCTGAACTC CATCATCCGC ACCTCGCCTA CATCTCTGGT GGCCTACATC 
ATCGGCTCCA GGATCTCACC AGCGTCCCAC AACACCCCGT CCCCACTGCA GGCGGACGTC 
TGTGGGCATC TGCTGGGCAT CAGGGGCAGC GGCATCCCTA CATCCAGCAA ACATAGCATC 
TCCGTCAGCG AGAGCCTCCG CATGCAGCCG CTGGAGGACC AGTTCACCAA CCTACTAGTG 
GAGCAGCAGA TCCTGCCAGA CCCACAAACT GCAAAGGCCA GTCAGACCCA TACGCTTCAG 
TTTGAGGTGT CCAGGATTGT CCGGTCCCCC AGCCCACCCC GGGGGCCACC ACCGCCCTAC 
AGCGCCCACC ACCAGCAGCA GCAGCAGCCA CGGGTTTTGC AGCTCCAACG GCCGGCGGGG 
CTTCAGCCGA TGCTGGAGGA GGAGGAAGAA GAGGAAGAGT CCTGCGGCGG GACACACTGC 
TGCTGCTGGC TGCACTGCGG AGCCATCTAC AGCCACCGGG ACGCGCTGGT CAAACACATC 
GAGAAGATCC ACGTGGACCA GCGCAAAGGA GAGGACTTCA CCTGCTTCTG GGCTGGCTGC 
GCGCGCAAAC ACAAACCCTT CAACGCACGC TACAAACTGC TGATCCACAT GAGGGTGCAC 
TCGGGGGAGA AGCCCAACAA GTGCTCGTTC GAGGGCTGTC AGAAGGCGTT TTCCCGCCTG 
GAGAACCTGA AGATCCACCT GAGGAGCCAC ACGGGTGAGA AACCCTACGT GTGTCCACAT 
CCCGGCTGCC TGAAAGCCTT CAGCAACTCC AGCGACAGAG CCAAACACCA GCGCACGCAT 
GTGGACACGA AACCGTACGC GTGTCAGATC CAGGGCTGTG GAAAGCGCTA CACCGACCCC 
AGCTCACTGC GGAAACACGT CAAATCTCAC TCGCTGAAGG AGCAGCAGGC CCGGAAGAAG 
TTGAGACGCA GCTCAGATCT CAGTCAGGAC GGACTCAGCG AGTGTTTGAC GGTTCAGCCG 
CTGCAGCACA GCCTCTCCAC ACTGGACCTG ATTGAGAGCA AGCACCACTC ACCCACAGAT 
GACCTCTACA CCGTCTTCAC CCTGAACCAG CCGAGCCAAA CAAACACCCT GAACAGCCCA 
ATGCAGATCG CAGGCGGTCC ACAGACTCAT CAAAGTCCTC ATCAGACTCA CCAGGCTCAT 
CAGATCCATC AGACTCAGAC TCATCTGACT CACCAGACCC ATCAGGCTCA CCAGACTCAG 
ACTCATCACA TCCAGCAGAT TCATCAGACT CACCTGACTC ATCAGATCCA GCAGATTCAC 
CAGACTCATC TCTCTCCACA TGGAGCACTG CCACCATTAC AGGGAAACAA CAGGTTTCTC 
AGCCCGAACA GCCACAAAGC TCCGGGATTC TGCAGTCAGC CCAGGACATC TGCTCAGGAC 
GGTTTCTCGG CTGTGTTTTC ATACGCCGAC TCAGACAAAC ACATCTCCTC CTGCTCCGTC 
ATGCAGACGC CCATGTTTGA GGACAATCTC AGCTCGTCGC CTCAGATCCA CAGCAACATC 
TCCGCAGTGC TGGACTGCCA GAGCGGCCCT CAAGGCTTTA TAGGAGAGCA GGAGCCCTCA 
GTGTCGGAGG ACAATTATCT TCACATCCCG AGATGTCCGA GCCGGATCTC CTGTGTGTTC 
ACTGAAGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303317.1
CDS	260..2671
Translation	MDMNGKGCNLMVSPSGMAQISPLKVHTPASLYPESNSAQSLRRQVLTNGKLGFPQPSRALHQQAPQISLPASSSSKQSARQCCQDSGMKGYRRVFGSCGSSNLQVSSSAFLVQSLSPASASVTHSSSQTCPQNTLTLPFNDARCLLSRESLASTTLSLAETQSVRSSKLDWPYGYRVLPPLGHQTANQLSESTVPESSLSESVSNPASLPHYLFKEDGSSPRLSARSKKRALSLSPLSDGLGLDLNSIIRTSPTSLVAYIIGSRISPASHNTPSPLQADVCGHLLGIRGSGIPTSSKHSISVSESLRMQPLEDQFTNLLVEQQILPDPQTAKASQTHTLQFEVSRIVRSPSPPRGPPPPYSAHHQQQQQPRVLQLQRPAGLQPMLEEEEEEEESCGGTHCCCWLHCGAIYSHRDALVKHIEKIHVDQRKGEDFTCFWAGCARKHKPFNARYKLLIHMRVHSGEKPNKCSFEGCQKAFSRLENLKIHLRSHTGEKPYVCPHPGCLKAFSNSSDRAKHQRTHVDTKPYACQIQGCGKRYTDPSSLRKHVKSHSLKEQQARKKLRRSSDLSQDGLSECLTVQPLQHSLSTLDLIESKHHSPTDDLYTVFTLNQPSQTNTLNSPMQIAGGPQTHQSPHQTHQAHQIHQTQTHLTHQTHQAHQTQTHHIQQIHQTHLTHQIQQIHQTHLSPHGALPPLQGNNRFLSPNSHKAPGFCSQPRTSAQDGFSAVFSYADSDKHISSCSVMQTPMFEDNLSSSPQIHSNISAVLDCQSGPQGFIGEQEPSVSEDNYLHIPRCPSRISCVFTEG

Target ORF information:

RefSeq Version	XM_009305042.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio GLIS family zinc finger 3 (glis3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305042.3

ORF Insert Sequence:

ATGGACATGA ATGGGAAAGG CTGTAACCTG ATGGTTTCTC CGTCCGGCAT GGCTCAGATA 
TCTCCGCTGA AGGTCCACAC TCCTGCATCT CTCTATCCAG AATCAAACTC AGCTCAGAGT 
CTCCGCCGGC AGGTGCTCAC CAATGGAAAA CTGGGCTTTC CACAGCCATC CCGAGCTCTT 
CATCAACAGG CTCCACAGAT TTCACTCCCA GCATCCTCCT CCTCCAAACA GTCTGCTCGA 
CAATGCTGTC AGGACAGCGG TATGAAAGGT TACAGAAGGG TGTTTGGGTC GTGTGGCAGC 
TCAAACCTGC AGGTCTCCAG CAGTGCTTTT CTGGTCCAGA GTCTCAGTCC TGCTTCTGCA 
TCCGTCACAC ACTCCTCATC ACAAACCTGC CCGCAGAATA CACTCACACT GCCCTTCAAT 
GACGCCAGGT GTTTGCTCTC CAGAGAGTCT CTGGCCTCCA CCACCCTTAG TCTGGCTGAG 
ACTCAGTCTG TGCGCAGCAG TAAACTGGAC TGGCCGTACG GCTACAGGGT CCTTCCTCCA 
CTAGGCCACC AAACAGCCAA TCAGTTGTCA GAATCCACTG TCCCTGAATC CTCCCTATCC 
GAAAGCGTCT CCAACCCTGC ATCTCTCCCT CATTACCTCT TCAAGGAAGA CGGCAGTAGT 
CCGCGCCTCT CGGCCCGCTC AAAGAAGCGA GCGCTGTCTT TATCCCCACT ATCGGATGGT 
TTGGGCCTGG ACCTGAACTC CATCATCCGC ACCTCGCCTA CATCTCTGGT GGCCTACATC 
ATCGGCTCCA GGATCTCACC AGCGTCCCAC AACACCCCGT CCCCACTGCA GGCGGACGTC 
TGTGGGCATC TGCTGGGCAT CAGGGGCAGC GGCATCCCTA CATCCAGCAA ACATAGCATC 
TCCGTCAGCG AGAGCCTCCG CATGCAGCCG CTGGAGGACC AGTTCACCAA CCTACTAGTG 
GAGCAGCAGA TCCTGCCAGA CCCACAAACT GCAAAGGCCA GTCAGACCCA TACGCTTCAG 
TTTGAGGTGT CCAGGATTGT CCGGTCCCCC AGCCCACCCC GGGGGCCACC ACCGCCCTAC 
AGCGCCCACC ACCAGCAGCA GCAGCAGCCA CGGGTTTTGC AGCTCCAACG GCCGGCGGGG 
CTTCAGCCGA TGCTGGAGGA GGAGGAAGAA GAGGAAGAGT CCTGCGGCGG GACACACTGC 
TGCTGCTGGC TGCACTGCGG AGCCATCTAC AGCCACCGGG ACGCGCTGGT CAAACACATC 
GAGAAGATCC ACGTGGACCA GCGCAAAGGA GAGGACTTCA CCTGCTTCTG GGCTGGCTGC 
GCGCGCAAAC ACAAACCCTT CAACGCACGC TACAAACTGC TGATCCACAT GAGGGTGCAC 
TCGGGGGAGA AGCCCAACAA GTGCTCGTTC GAGGGCTGTC AGAAGGCGTT TTCCCGCCTG 
GAGAACCTGA AGATCCACCT GAGGAGCCAC ACGGGTGAGA AACCCTACGT GTGTCCACAT 
CCCGGCTGCC TGAAAGCCTT CAGCAACTCC AGCGACAGAG CCAAACACCA GCGCACGCAT 
GTGGACACGA AACCGTACGC GTGTCAGATC CAGGGCTGTG GAAAGCGCTA CACCGACCCC 
AGCTCACTGC GGAAACACGT CAAATCTCAC TCGCTGAAGG AGCAGCAGGC CCGGAAGAAG 
TTGAGACGCA GCTCAGATCT CAGTCAGGAC GGACTCAGCG AGTGTTTGAC GGTTCAGCCG 
CTGCAGCACA GCCTCTCCAC ACTGGACCTG ATTGAGAGCA AGCACCACTC ACCCACAGAT 
GACCTCTACA CCGTCTTCAC CCTGAACCAG CCGAGCCAAA CAAACACCCT GAACAGCCCA 
ATGCAGATCG CAGGCGGTCC ACAGACTCAT CAAAGTCCTC ATCAGACTCA CCAGGCTCAT 
CAGATCCATC AGACTCAGAC TCATCTGACT CACCAGACCC ATCAGGCTCA CCAGACTCAG 
ACTCATCACA TCCAGCAGAT TCATCAGACT CACCTGACTC ATCAGATCCA GCAGATTCAC 
CAGACTCATC TCTCTCCACA TGGAGCACTG CCACCATTAC AGGGAAACAA CAGGTTTCTC 
AGCCCGAACA GCCACAAAGC TCCGGGATTC TGCAGTCAGC CCAGGACATC TGCTCAGGAC 
GGTTTCTCGG CTGTGTTTTC ATACGCCGAC TCAGACAAAC ACATCTCCTC CTGCTCCGTC 
ATGCAGACGC CCATGTTTGA GGACAATCTC AGCTCGTCGC CTCAGATCCA CAGCAACATC 
TCCGCAGTGC TGGACTGCCA GAGCGGCCCT CAAGGCTTTA TAGGAGAGCA GGAGCCCTCA 
GTGTCGGAGG ACAATTATCT TCACATCCCG AGATGTCCGA GCCGGATCTC CTGTGTGTTC 
ACTGAAGGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

glis3 ( XM_017357981.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213470.1 Danio rerio GLIS family zinc finger 3 (glis3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa54032
Clone ID Related Accession (Same CDS sequence)	XM_017357981.2 , XM_017357981.1
Accession Version	XM_017357981.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2415bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	zinc finger protein GLIS3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357981.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATGA ATGGGAAAGG CTGTAACCTG ATGGTTTCTC CGTCCGGCAT GGCTCAGATA 
TCTCCGCTGA AGGTCCACAC TCCTGCATCT CTCTATCCAG AATCAAACTC AGCTCAGAGT 
CTCCGCCGGC AGGTGCTCAC CAATGGAAAA CTGGGCTTTC CACAGCCATC CCGAGCTCTT 
CATCAACAGG CTCCACAGAT TTCACTCCCA GCATCCTCCT CCTCCAAACA GTCTGCTCGA 
CAATGCTGTC AGGACAGCGG TATGAAAGGT TACAGAAGGG TGTTTGGGTC GTGTGGCAGC 
TCAAACCTGC AGGTCTCCAG CAGTGCTTTT CTGGTCCAGA GTCTCAGTCC TGCTTCTGCA 
TCCGTCACAC ACTCCTCATC ACAAACCTGC CCGCAGAATA CACTCACACT GCCCTTCAAT 
GACGCCAGCA GGTGTTTGCT CTCCAGAGAG TCTCTGGCCT CCACCACCCT TAGTCTGGCT 
GAGACTCAGT CTGTGCGCAG CAGTAAACTG GACTGGCCGT ACGGCTACAG GGTCCTTCCT 
CCACTAGGCC ACCAAACAGC CAATCAGTTG TCAGAATCCA CTGTCCCTGA ATCCTCCCTA 
TCCGAAAGCG TCTCCAACCC TGCATCTCTC CCTCATTACC TCTTCAAGGA AGACGGCAGT 
AGTCCGCGCC TCTCGGCCCG CTCAAAGAAG CGAGCGCTGT CTTTATCCCC ACTATCGGAT 
GGTTTGGGCC TGGACCTGAA CTCCATCATC CGCACCTCGC CTACATCTCT GGTGGCCTAC 
ATCATCGGCT CCAGGATCTC ACCAGCGTCC CACAACACCC CGTCCCCACT GCAGGCGGAC 
GTCTGTGGGC ATCTGCTGGG CATCAGGGGC AGCGGCATCC CTACATCCAG CAAACATAGC 
ATCTCCGTCA GCGAGAGCCT CCGCATGCAG CCGCTGGAGG ACCAGTTCAC CAACCTACTA 
GTGGAGCAGC AGATCCTGCC AGACCCACAA ACTGCAAAGG CCAGTCAGAC CCATACGCTT 
CAGTTTGAGG TGTCCAGGAT TGTCCGGTCC CCCAGCCCAC CCCGGGGGCC ACCACCGCCC 
TACAGCGCCC ACCACCAGCA GCAGCAGCAG CCACGGGTTT TGCAGCTCCA ACGGCCGGCG 
GGGCTTCAGC CGATGCTGGA GGAGGAGGAA GAAGAGGAAG AGTCCTGCGG CGGGACACAC 
TGCTGCTGCT GGCTGCACTG CGGAGCCATC TACAGCCACC GGGACGCGCT GGTCAAACAC 
ATCGAGAAGA TCCACGTGGA CCAGCGCAAA GGAGAGGACT TCACCTGCTT CTGGGCTGGC 
TGCGCGCGCA AACACAAACC CTTCAACGCA CGCTACAAAC TGCTGATCCA CATGAGGGTG 
CACTCGGGGG AGAAGCCCAA CAAGTGCTCG TTCGAGGGCT GTCAGAAGGC GTTTTCCCGC 
CTGGAGAACC TGAAGATCCA CCTGAGGAGC CACACGGGTG AGAAACCCTA CGTGTGTCCA 
CATCCCGGCT GCCTGAAAGC CTTCAGCAAC TCCAGCGACA GAGCCAAACA CCAGCGCACG 
CATGTGGACA CGAAACCGTA CGCGTGTCAG ATCCAGGGCT GTGGAAAGCG CTACACCGAC 
CCCAGCTCAC TGCGGAAACA CGTCAAATCT CACTCGCTGA AGGAGCAGCA GGCCCGGAAG 
AAGTTGAGAC GCAGCTCAGA TCTCAGTCAG GACGGACTCA GCGAGTGTTT GACGGTTCAG 
CCGCTGCAGC ACAGCCTCTC CACACTGGAC CTGATTGAGA GCAAGCACCA CTCACCCACA 
GATGACCTCT ACACCGTCTT CACCCTGAAC CAGCCGAGCC AAACAAACAC CCTGAACAGC 
CCAATGCAGA TCGCAGGCGG TCCACAGACT CATCAAAGTC CTCATCAGAC TCACCAGGCT 
CATCAGATCC ATCAGACTCA GACTCATCTG ACTCACCAGA CCCATCAGGC TCACCAGACT 
CAGACTCATC ACATCCAGCA GATTCATCAG ACTCACCTGA CTCATCAGAT CCAGCAGATT 
CACCAGACTC ATCTCTCTCC ACATGGAGCA CTGCCACCAT TACAGGGAAA CAACAGGTTT 
CTCAGCCCGA ACAGCCACAA AGCTCCGGGA TTCTGCAGTC AGCCCAGGAC ATCTGCTCAG 
GACGGTTTCT CGGCTGTGTT TTCATACGCC GACTCAGACA AACACATCTC CTCCTGCTCC 
GTCATGCAGA CGCCCATGTT TGAGGACAAT CTCAGCTCGT CGCCTCAGAT CCACAGCAAC 
ATCTCCGCAG TGCTGGACTG CCAGAGCGGC CCTCAAGGCT TTATAGGAGA GCAGGAGCCC 
TCAGTGTCGG AGGACAATTA TCTTCACATC CCGAGATGTC CGAGCCGGAT CTCCTGTGTG 
TTCACTGAAG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213470.1
CDS	260..2674
Translation	MDMNGKGCNLMVSPSGMAQISPLKVHTPASLYPESNSAQSLRRQVLTNGKLGFPQPSRALHQQAPQISLPASSSSKQSARQCCQDSGMKGYRRVFGSCGSSNLQVSSSAFLVQSLSPASASVTHSSSQTCPQNTLTLPFNDASRCLLSRESLASTTLSLAETQSVRSSKLDWPYGYRVLPPLGHQTANQLSESTVPESSLSESVSNPASLPHYLFKEDGSSPRLSARSKKRALSLSPLSDGLGLDLNSIIRTSPTSLVAYIIGSRISPASHNTPSPLQADVCGHLLGIRGSGIPTSSKHSISVSESLRMQPLEDQFTNLLVEQQILPDPQTAKASQTHTLQFEVSRIVRSPSPPRGPPPPYSAHHQQQQQPRVLQLQRPAGLQPMLEEEEEEEESCGGTHCCCWLHCGAIYSHRDALVKHIEKIHVDQRKGEDFTCFWAGCARKHKPFNARYKLLIHMRVHSGEKPNKCSFEGCQKAFSRLENLKIHLRSHTGEKPYVCPHPGCLKAFSNSSDRAKHQRTHVDTKPYACQIQGCGKRYTDPSSLRKHVKSHSLKEQQARKKLRRSSDLSQDGLSECLTVQPLQHSLSTLDLIESKHHSPTDDLYTVFTLNQPSQTNTLNSPMQIAGGPQTHQSPHQTHQAHQIHQTQTHLTHQTHQAHQTQTHHIQQIHQTHLTHQIQQIHQTHLSPHGALPPLQGNNRFLSPNSHKAPGFCSQPRTSAQDGFSAVFSYADSDKHISSCSVMQTPMFEDNLSSSPQIHSNISAVLDCQSGPQGFIGEQEPSVSEDNYLHIPRCPSRISCVFTEG

Target ORF information:

RefSeq Version	XM_017357981.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio GLIS family zinc finger 3 (glis3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357981.2

ORF Insert Sequence:

ATGGACATGA ATGGGAAAGG CTGTAACCTG ATGGTTTCTC CGTCCGGCAT GGCTCAGATA 
TCTCCGCTGA AGGTCCACAC TCCTGCATCT CTCTATCCAG AATCAAACTC AGCTCAGAGT 
CTCCGCCGGC AGGTGCTCAC CAATGGAAAA CTGGGCTTTC CACAGCCATC CCGAGCTCTT 
CATCAACAGG CTCCACAGAT TTCACTCCCA GCATCCTCCT CCTCCAAACA GTCTGCTCGA 
CAATGCTGTC AGGACAGCGG TATGAAAGGT TACAGAAGGG TGTTTGGGTC GTGTGGCAGC 
TCAAACCTGC AGGTCTCCAG CAGTGCTTTT CTGGTCCAGA GTCTCAGTCC TGCTTCTGCA 
TCCGTCACAC ACTCCTCATC ACAAACCTGC CCGCAGAATA CACTCACACT GCCCTTCAAT 
GACGCCAGCA GGTGTTTGCT CTCCAGAGAG TCTCTGGCCT CCACCACCCT TAGTCTGGCT 
GAGACTCAGT CTGTGCGCAG CAGTAAACTG GACTGGCCGT ACGGCTACAG GGTCCTTCCT 
CCACTAGGCC ACCAAACAGC CAATCAGTTG TCAGAATCCA CTGTCCCTGA ATCCTCCCTA 
TCCGAAAGCG TCTCCAACCC TGCATCTCTC CCTCATTACC TCTTCAAGGA AGACGGCAGT 
AGTCCGCGCC TCTCGGCCCG CTCAAAGAAG CGAGCGCTGT CTTTATCCCC ACTATCGGAT 
GGTTTGGGCC TGGACCTGAA CTCCATCATC CGCACCTCGC CTACATCTCT GGTGGCCTAC 
ATCATCGGCT CCAGGATCTC ACCAGCGTCC CACAACACCC CGTCCCCACT GCAGGCGGAC 
GTCTGTGGGC ATCTGCTGGG CATCAGGGGC AGCGGCATCC CTACATCCAG CAAACATAGC 
ATCTCCGTCA GCGAGAGCCT CCGCATGCAG CCGCTGGAGG ACCAGTTCAC CAACCTACTA 
GTGGAGCAGC AGATCCTGCC AGACCCACAA ACTGCAAAGG CCAGTCAGAC CCATACGCTT 
CAGTTTGAGG TGTCCAGGAT TGTCCGGTCC CCCAGCCCAC CCCGGGGGCC ACCACCGCCC 
TACAGCGCCC ACCACCAGCA GCAGCAGCAG CCACGGGTTT TGCAGCTCCA ACGGCCGGCG 
GGGCTTCAGC CGATGCTGGA GGAGGAGGAA GAAGAGGAAG AGTCCTGCGG CGGGACACAC 
TGCTGCTGCT GGCTGCACTG CGGAGCCATC TACAGCCACC GGGACGCGCT GGTCAAACAC 
ATCGAGAAGA TCCACGTGGA CCAGCGCAAA GGAGAGGACT TCACCTGCTT CTGGGCTGGC 
TGCGCGCGCA AACACAAACC CTTCAACGCA CGCTACAAAC TGCTGATCCA CATGAGGGTG 
CACTCGGGGG AGAAGCCCAA CAAGTGCTCG TTCGAGGGCT GTCAGAAGGC GTTTTCCCGC 
CTGGAGAACC TGAAGATCCA CCTGAGGAGC CACACGGGTG AGAAACCCTA CGTGTGTCCA 
CATCCCGGCT GCCTGAAAGC CTTCAGCAAC TCCAGCGACA GAGCCAAACA CCAGCGCACG 
CATGTGGACA CGAAACCGTA CGCGTGTCAG ATCCAGGGCT GTGGAAAGCG CTACACCGAC 
CCCAGCTCAC TGCGGAAACA CGTCAAATCT CACTCGCTGA AGGAGCAGCA GGCCCGGAAG 
AAGTTGAGAC GCAGCTCAGA TCTCAGTCAG GACGGACTCA GCGAGTGTTT GACGGTTCAG 
CCGCTGCAGC ACAGCCTCTC CACACTGGAC CTGATTGAGA GCAAGCACCA CTCACCCACA 
GATGACCTCT ACACCGTCTT CACCCTGAAC CAGCCGAGCC AAACAAACAC CCTGAACAGC 
CCAATGCAGA TCGCAGGCGG TCCACAGACT CATCAAAGTC CTCATCAGAC TCACCAGGCT 
CATCAGATCC ATCAGACTCA GACTCATCTG ACTCACCAGA CCCATCAGGC TCACCAGACT 
CAGACTCATC ACATCCAGCA GATTCATCAG ACTCACCTGA CTCATCAGAT CCAGCAGATT 
CACCAGACTC ATCTCTCTCC ACATGGAGCA CTGCCACCAT TACAGGGAAA CAACAGGTTT 
CTCAGCCCGA ACAGCCACAA AGCTCCGGGA TTCTGCAGTC AGCCCAGGAC ATCTGCTCAG 
GACGGTTTCT CGGCTGTGTT TTCATACGCC GACTCAGACA AACACATCTC CTCCTGCTCC 
GTCATGCAGA CGCCCATGTT TGAGGACAAT CTCAGCTCGT CGCCTCAGAT CCACAGCAAC 
ATCTCCGCAG TGCTGGACTG CCAGAGCGGC CCTCAAGGCT TTATAGGAGA GCAGGAGCCC 
TCAGTGTCGG AGGACAATTA TCTTCACATC CCGAGATGTC CGAGCCGGAT CTCCTGTGTG 
TTCACTGAAG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

glis3 ( XM_017357981.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213470.1 Danio rerio GLIS family zinc finger 3 (glis3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa54032
Clone ID Related Accession (Same CDS sequence)	XM_017357981.2 , XM_017357981.1
Accession Version	XM_017357981.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2415bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	zinc finger protein GLIS3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATGA ATGGGAAAGG CTGTAACCTG ATGGTTTCTC CGTCCGGCAT GGCTCAGATA 
TCTCCGCTGA AGGTCCACAC TCCTGCATCT CTCTATCCAG AATCAAACTC AGCTCAGAGT 
CTCCGCCGGC AGGTGCTCAC CAATGGAAAA CTGGGCTTTC CACAGCCATC CCGAGCTCTT 
CATCAACAGG CTCCACAGAT TTCACTCCCA GCATCCTCCT CCTCCAAACA GTCTGCTCGA 
CAATGCTGTC AGGACAGCGG TATGAAAGGT TACAGAAGGG TGTTTGGGTC GTGTGGCAGC 
TCAAACCTGC AGGTCTCCAG CAGTGCTTTT CTGGTCCAGA GTCTCAGTCC TGCTTCTGCA 
TCCGTCACAC ACTCCTCATC ACAAACCTGC CCGCAGAATA CACTCACACT GCCCTTCAAT 
GACGCCAGCA GGTGTTTGCT CTCCAGAGAG TCTCTGGCCT CCACCACCCT TAGTCTGGCT 
GAGACTCAGT CTGTGCGCAG CAGTAAACTG GACTGGCCGT ACGGCTACAG GGTCCTTCCT 
CCACTAGGCC ACCAAACAGC CAATCAGTTG TCAGAATCCA CTGTCCCTGA ATCCTCCCTA 
TCCGAAAGCG TCTCCAACCC TGCATCTCTC CCTCATTACC TCTTCAAGGA AGACGGCAGT 
AGTCCGCGCC TCTCGGCCCG CTCAAAGAAG CGAGCGCTGT CTTTATCCCC ACTATCGGAT 
GGTTTGGGCC TGGACCTGAA CTCCATCATC CGCACCTCGC CTACATCTCT GGTGGCCTAC 
ATCATCGGCT CCAGGATCTC ACCAGCGTCC CACAACACCC CGTCCCCACT GCAGGCGGAC 
GTCTGTGGGC ATCTGCTGGG CATCAGGGGC AGCGGCATCC CTACATCCAG CAAACATAGC 
ATCTCCGTCA GCGAGAGCCT CCGCATGCAG CCGCTGGAGG ACCAGTTCAC CAACCTACTA 
GTGGAGCAGC AGATCCTGCC AGACCCACAA ACTGCAAAGG CCAGTCAGAC CCATACGCTT 
CAGTTTGAGG TGTCCAGGAT TGTCCGGTCC CCCAGCCCAC CCCGGGGGCC ACCACCGCCC 
TACAGCGCCC ACCACCAGCA GCAGCAGCAG CCACGGGTTT TGCAGCTCCA ACGGCCGGCG 
GGGCTTCAGC CGATGCTGGA GGAGGAGGAA GAAGAGGAAG AGTCCTGCGG CGGGACACAC 
TGCTGCTGCT GGCTGCACTG CGGAGCCATC TACAGCCACC GGGACGCGCT GGTCAAACAC 
ATCGAGAAGA TCCACGTGGA CCAGCGCAAA GGAGAGGACT TCACCTGCTT CTGGGCTGGC 
TGCGCGCGCA AACACAAACC CTTCAACGCA CGCTACAAAC TGCTGATCCA CATGAGGGTG 
CACTCGGGGG AGAAGCCCAA CAAGTGCTCG TTCGAGGGCT GTCAGAAGGC GTTTTCCCGC 
CTGGAGAACC TGAAGATCCA CCTGAGGAGC CACACGGGTG AGAAACCCTA CGTGTGTCCA 
CATCCCGGCT GCCTGAAAGC CTTCAGCAAC TCCAGCGACA GAGCCAAACA CCAGCGCACG 
CATGTGGACA CGAAACCGTA CGCGTGTCAG ATCCAGGGCT GTGGAAAGCG CTACACCGAC 
CCCAGCTCAC TGCGGAAACA CGTCAAATCT CACTCGCTGA AGGAGCAGCA GGCCCGGAAG 
AAGTTGAGAC GCAGCTCAGA TCTCAGTCAG GACGGACTCA GCGAGTGTTT GACGGTTCAG 
CCGCTGCAGC ACAGCCTCTC CACACTGGAC CTGATTGAGA GCAAGCACCA CTCACCCACA 
GATGACCTCT ACACCGTCTT CACCCTGAAC CAGCCGAGCC AAACAAACAC CCTGAACAGC 
CCAATGCAGA TCGCAGGCGG TCCACAGACT CATCAAAGTC CTCATCAGAC TCACCAGGCT 
CATCAGATCC ATCAGACTCA GACTCATCTG ACTCACCAGA CCCATCAGGC TCACCAGACT 
CAGACTCATC ACATCCAGCA GATTCATCAG ACTCACCTGA CTCATCAGAT CCAGCAGATT 
CACCAGACTC ATCTCTCTCC ACATGGAGCA CTGCCACCAT TACAGGGAAA CAACAGGTTT 
CTCAGCCCGA ACAGCCACAA AGCTCCGGGA TTCTGCAGTC AGCCCAGGAC ATCTGCTCAG 
GACGGTTTCT CGGCTGTGTT TTCATACGCC GACTCAGACA AACACATCTC CTCCTGCTCC 
GTCATGCAGA CGCCCATGTT TGAGGACAAT CTCAGCTCGT CGCCTCAGAT CCACAGCAAC 
ATCTCCGCAG TGCTGGACTG CCAGAGCGGC CCTCAAGGCT TTATAGGAGA GCAGGAGCCC 
TCAGTGTCGG AGGACAATTA TCTTCACATC CCGAGATGTC CGAGCCGGAT CTCCTGTGTG 
TTCACTGAAG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213470.1
CDS	249..2663
Translation	MDMNGKGCNLMVSPSGMAQISPLKVHTPASLYPESNSAQSLRRQVLTNGKLGFPQPSRALHQQAPQISLPASSSSKQSARQCCQDSGMKGYRRVFGSCGSSNLQVSSSAFLVQSLSPASASVTHSSSQTCPQNTLTLPFNDASRCLLSRESLASTTLSLAETQSVRSSKLDWPYGYRVLPPLGHQTANQLSESTVPESSLSESVSNPASLPHYLFKEDGSSPRLSARSKKRALSLSPLSDGLGLDLNSIIRTSPTSLVAYIIGSRISPASHNTPSPLQADVCGHLLGIRGSGIPTSSKHSISVSESLRMQPLEDQFTNLLVEQQILPDPQTAKASQTHTLQFEVSRIVRSPSPPRGPPPPYSAHHQQQQQPRVLQLQRPAGLQPMLEEEEEEEESCGGTHCCCWLHCGAIYSHRDALVKHIEKIHVDQRKGEDFTCFWAGCARKHKPFNARYKLLIHMRVHSGEKPNKCSFEGCQKAFSRLENLKIHLRSHTGEKPYVCPHPGCLKAFSNSSDRAKHQRTHVDTKPYACQIQGCGKRYTDPSSLRKHVKSHSLKEQQARKKLRRSSDLSQDGLSECLTVQPLQHSLSTLDLIESKHHSPTDDLYTVFTLNQPSQTNTLNSPMQIAGGPQTHQSPHQTHQAHQIHQTQTHLTHQTHQAHQTQTHHIQQIHQTHLTHQIQQIHQTHLSPHGALPPLQGNNRFLSPNSHKAPGFCSQPRTSAQDGFSAVFSYADSDKHISSCSVMQTPMFEDNLSSSPQIHSNISAVLDCQSGPQGFIGEQEPSVSEDNYLHIPRCPSRISCVFTEG

Target ORF information:

RefSeq Version	XM_017357981.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio GLIS family zinc finger 3 (glis3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357981.1

ORF Insert Sequence:

ATGGACATGA ATGGGAAAGG CTGTAACCTG ATGGTTTCTC CGTCCGGCAT GGCTCAGATA 
TCTCCGCTGA AGGTCCACAC TCCTGCATCT CTCTATCCAG AATCAAACTC AGCTCAGAGT 
CTCCGCCGGC AGGTGCTCAC CAATGGAAAA CTGGGCTTTC CACAGCCATC CCGAGCTCTT 
CATCAACAGG CTCCACAGAT TTCACTCCCA GCATCCTCCT CCTCCAAACA GTCTGCTCGA 
CAATGCTGTC AGGACAGCGG TATGAAAGGT TACAGAAGGG TGTTTGGGTC GTGTGGCAGC 
TCAAACCTGC AGGTCTCCAG CAGTGCTTTT CTGGTCCAGA GTCTCAGTCC TGCTTCTGCA 
TCCGTCACAC ACTCCTCATC ACAAACCTGC CCGCAGAATA CACTCACACT GCCCTTCAAT 
GACGCCAGCA GGTGTTTGCT CTCCAGAGAG TCTCTGGCCT CCACCACCCT TAGTCTGGCT 
GAGACTCAGT CTGTGCGCAG CAGTAAACTG GACTGGCCGT ACGGCTACAG GGTCCTTCCT 
CCACTAGGCC ACCAAACAGC CAATCAGTTG TCAGAATCCA CTGTCCCTGA ATCCTCCCTA 
TCCGAAAGCG TCTCCAACCC TGCATCTCTC CCTCATTACC TCTTCAAGGA AGACGGCAGT 
AGTCCGCGCC TCTCGGCCCG CTCAAAGAAG CGAGCGCTGT CTTTATCCCC ACTATCGGAT 
GGTTTGGGCC TGGACCTGAA CTCCATCATC CGCACCTCGC CTACATCTCT GGTGGCCTAC 
ATCATCGGCT CCAGGATCTC ACCAGCGTCC CACAACACCC CGTCCCCACT GCAGGCGGAC 
GTCTGTGGGC ATCTGCTGGG CATCAGGGGC AGCGGCATCC CTACATCCAG CAAACATAGC 
ATCTCCGTCA GCGAGAGCCT CCGCATGCAG CCGCTGGAGG ACCAGTTCAC CAACCTACTA 
GTGGAGCAGC AGATCCTGCC AGACCCACAA ACTGCAAAGG CCAGTCAGAC CCATACGCTT 
CAGTTTGAGG TGTCCAGGAT TGTCCGGTCC CCCAGCCCAC CCCGGGGGCC ACCACCGCCC 
TACAGCGCCC ACCACCAGCA GCAGCAGCAG CCACGGGTTT TGCAGCTCCA ACGGCCGGCG 
GGGCTTCAGC CGATGCTGGA GGAGGAGGAA GAAGAGGAAG AGTCCTGCGG CGGGACACAC 
TGCTGCTGCT GGCTGCACTG CGGAGCCATC TACAGCCACC GGGACGCGCT GGTCAAACAC 
ATCGAGAAGA TCCACGTGGA CCAGCGCAAA GGAGAGGACT TCACCTGCTT CTGGGCTGGC 
TGCGCGCGCA AACACAAACC CTTCAACGCA CGCTACAAAC TGCTGATCCA CATGAGGGTG 
CACTCGGGGG AGAAGCCCAA CAAGTGCTCG TTCGAGGGCT GTCAGAAGGC GTTTTCCCGC 
CTGGAGAACC TGAAGATCCA CCTGAGGAGC CACACGGGTG AGAAACCCTA CGTGTGTCCA 
CATCCCGGCT GCCTGAAAGC CTTCAGCAAC TCCAGCGACA GAGCCAAACA CCAGCGCACG 
CATGTGGACA CGAAACCGTA CGCGTGTCAG ATCCAGGGCT GTGGAAAGCG CTACACCGAC 
CCCAGCTCAC TGCGGAAACA CGTCAAATCT CACTCGCTGA AGGAGCAGCA GGCCCGGAAG 
AAGTTGAGAC GCAGCTCAGA TCTCAGTCAG GACGGACTCA GCGAGTGTTT GACGGTTCAG 
CCGCTGCAGC ACAGCCTCTC CACACTGGAC CTGATTGAGA GCAAGCACCA CTCACCCACA 
GATGACCTCT ACACCGTCTT CACCCTGAAC CAGCCGAGCC AAACAAACAC CCTGAACAGC 
CCAATGCAGA TCGCAGGCGG TCCACAGACT CATCAAAGTC CTCATCAGAC TCACCAGGCT 
CATCAGATCC ATCAGACTCA GACTCATCTG ACTCACCAGA CCCATCAGGC TCACCAGACT 
CAGACTCATC ACATCCAGCA GATTCATCAG ACTCACCTGA CTCATCAGAT CCAGCAGATT 
CACCAGACTC ATCTCTCTCC ACATGGAGCA CTGCCACCAT TACAGGGAAA CAACAGGTTT 
CTCAGCCCGA ACAGCCACAA AGCTCCGGGA TTCTGCAGTC AGCCCAGGAC ATCTGCTCAG 
GACGGTTTCT CGGCTGTGTT TTCATACGCC GACTCAGACA AACACATCTC CTCCTGCTCC 
GTCATGCAGA CGCCCATGTT TGAGGACAAT CTCAGCTCGT CGCCTCAGAT CCACAGCAAC 
ATCTCCGCAG TGCTGGACTG CCAGAGCGGC CCTCAAGGCT TTATAGGAGA GCAGGAGCCC 
TCAGTGTCGG AGGACAATTA TCTTCACATC CCGAGATGTC CGAGCCGGAT CTCCTGTGTG 
TTCACTGAAG GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Assignment of Functional Relevance to Genes at Type 2 Diabetes-Associated Loci Through Investigation of ?-Cell Mass Deficits.
Molecular endocrinology (Baltimore, Md.)30(4)429-45(2016 Apr)
O'Hare EA,Yerges-Armstrong LM,Perry JA,Shuldiner AR,Zaghloul NA

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Identification and expression analysis of zebrafish polypeptide ?-N-acetylgalactosaminyltransferase Y-subfamily genes during embryonic development.
Gene expression patterns : GEP16(1)1-7(2014 Sep)
Nakayama Y,Nakamura N,Kawai T,Kaneda E,Takahashi Y,Miyake A,Itoh N,Kurosaka A

Systematic discovery of novel ciliary genes through functional genomics in the zebrafish.
Development (Cambridge, England)141(17)3410-9(2014 Sep)
Choksi SP,Babu D,Lau D,Yu X,Roy S

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glis3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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