fhod3b cDNA ORF clone, Danio rerio(zebrafish)

The following fhod3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the fhod3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa145776 XM_021472977.1
Latest version!
Danio rerio formin homology 2 domain containing 3b (fhod3b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $104.30
$149.00
ODa145777 XM_021472978.1
Latest version!
Danio rerio formin homology 2 domain containing 3b (fhod3b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $104.30
$149.00
ODa149376 XM_021466891.1
Latest version!
Danio rerio formin homology 2 domain containing 3b (fhod3b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa149377 XM_021466892.1
Latest version!
Danio rerio formin homology 2 domain containing 3b (fhod3b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa149378 XM_021466893.1
Latest version!
Danio rerio formin homology 2 domain containing 3b (fhod3b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa149379 XM_021466894.1
Latest version!
Danio rerio formin homology 2 domain containing 3b (fhod3b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa21317 XM_005157949.3
Latest version!
Danio rerio formin homology 2 domain containing 3b (fhod3b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa21319 XM_005157951.3
Latest version!
Danio rerio formin homology 2 domain containing 3b (fhod3b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa46042 XM_009292259.2
Latest version!
Danio rerio formin homology 2 domain containing 3b (fhod3b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa46043 XM_009292260.2
Latest version!
Danio rerio formin homology 2 domain containing 3b (fhod3b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa46044 XM_009292261.2
Latest version!
Danio rerio formin homology 2 domain containing 3b (fhod3b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa145776
    Clone ID Related Accession (Same CDS sequence) XM_021472977.1
    Accession Version XM_021472977.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 444bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product FH1/FH2 domain-containing protein 3 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394959.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    ATGACTTTGT GTTGTCAGAG TGGGAAGTTT GCAGGAAGTC CTATGGAGGG TCAGGAGTGT 
    CAACCGCAGG GTTTGGGCTA CACAGAGGAC GTGACTGAGC ATGAGCAGAT GACCGCAGTG
    CTTTGTACCA GCCTGCAGGG TGGCGATAGT CCATCATCTG TCCTTCCTGG CCTTCGCACA
    CGGACCCGAG CCACTCGAGG TCATACAGCA CTTTTGTCCA CTGCAAATGA AGACACTTCT
    AATTGCACAA ATGACACAGC AGATGAGATC ATGGAGCGGA TTGTGAAATC AGCCACTCAG
    GGCCCCAGTC AGAGAACACA GCCTCGTGAG AGGAGACGTT CCAGAGCAAA CCGCAAATCC
    TTGAGAAGAA CACTGAAGAA CGGTCTTACC CCGGAGGAGG CACAGGCTCT GGGTTTGGCC
    AGCGGCTCTG AGATGCAGGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021328652.1
    CDS563..1006
    Translation

    Target ORF information:

    RefSeq Version XM_021472977.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio formin homology 2 domain containing 3b (fhod3b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021472977.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    ATGACTTTGT GTTGTCAGAG TGGGAAGTTT GCAGGAAGTC CTATGGAGGG TCAGGAGTGT 
    CAACCGCAGG GTTTGGGCTA CACAGAGGAC GTGACTGAGC ATGAGCAGAT GACCGCAGTG
    CTTTGTACCA GCCTGCAGGG TGGCGATAGT CCATCATCTG TCCTTCCTGG CCTTCGCACA
    CGGACCCGAG CCACTCGAGG TCATACAGCA CTTTTGTCCA CTGCAAATGA AGACACTTCT
    AATTGCACAA ATGACACAGC AGATGAGATC ATGGAGCGGA TTGTGAAATC AGCCACTCAG
    GGCCCCAGTC AGAGAACACA GCCTCGTGAG AGGAGACGTT CCAGAGCAAA CCGCAAATCC
    TTGAGAAGAA CACTGAAGAA CGGTCTTACC CCGGAGGAGG CACAGGCTCT GGGTTTGGCC
    AGCGGCTCTG AGATGCAGGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa145777
    Clone ID Related Accession (Same CDS sequence) XM_021472978.1
    Accession Version XM_021472978.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 402bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product FH1/FH2 domain-containing protein 3 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394959.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    ATGGAGGGTC AGGAGTGTCA ACCGCAGGGT TTGGGCTACA CAGAGGACGT GACTGAGCAT 
    GAGCAGATGA CCGCAGTGCT TTGTACCAGC CTGCAGGGTG GCGATAGTCC ATCATCTGTC
    CTTCCTGGCC TTCGCACACG GACCCGAGCC ACTCGAGGTC ATACAGCACT TTTGTCCACT
    GCAAATGAAG ACACTTCTAA TTGCACAAAT GACACAGCAG ATGAGATCAT GGAGCGGATT
    GTGAAATCAG CCACTCAGGG CCCCAGTCAG AGAACACAGC CTCGTGAGAG GAGACGTTCC
    AGAGCAAACC GCAAATCCTT GAGAAGAACA CTGAAGAACG GTCTTACCCC GGAGGAGGCA
    CAGGCTCTGG GTTTGGCCAG CGGCTCTGAG ATGCAGGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021328653.1
    CDS241..642
    Translation

    Target ORF information:

    RefSeq Version XM_021472978.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio formin homology 2 domain containing 3b (fhod3b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021472978.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    ATGGAGGGTC AGGAGTGTCA ACCGCAGGGT TTGGGCTACA CAGAGGACGT GACTGAGCAT 
    GAGCAGATGA CCGCAGTGCT TTGTACCAGC CTGCAGGGTG GCGATAGTCC ATCATCTGTC
    CTTCCTGGCC TTCGCACACG GACCCGAGCC ACTCGAGGTC ATACAGCACT TTTGTCCACT
    GCAAATGAAG ACACTTCTAA TTGCACAAAT GACACAGCAG ATGAGATCAT GGAGCGGATT
    GTGAAATCAG CCACTCAGGG CCCCAGTCAG AGAACACAGC CTCGTGAGAG GAGACGTTCC
    AGAGCAAACC GCAAATCCTT GAGAAGAACA CTGAAGAACG GTCTTACCCC GGAGGAGGCA
    CAGGCTCTGG GTTTGGCCAG CGGCTCTGAG ATGCAGGTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149376
    Clone ID Related Accession (Same CDS sequence) XM_021466891.1
    Accession Version XM_021466891.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5079bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product FH1/FH2 domain-containing protein 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCTACGT TTGCATGCAG GGTGCAGTTC TTAGATGATA CAGATCCATT TAATAGCACA 
    AATTTTCCGG AGCCGACCAG ACCCCCACAT TTCACATTCA GAGAGGATAT TCCGCTCATC
    AATCAGATCG CCGGAGTTCA CAGACTTCTC AAAGCACCTC ACAAGCTTGA TGACTGTGCT
    TTGCAGTTGT CTCATAATGG CAGTTATCTG GACCTGGAGT CCACCTTGGC TGAGCAGAAA
    GATGAACTTG AAGGCTTTCA GGAGGATGGA GGGAGAGGAA AGAAGCACAG TATTGTACTT
    AGAACACAGC TGTCTGTCAG AGTCCATGCC TGCATAGAAA AGCTGTACAA CTCGACGGGT
    CGTGAGCTAC GAAGAGCACT GTTCTCTCTC AAGCAAATCT TCCAGGATGA TAAGGATCTG
    GTTCATGAGT TTGTGATGGC GGAGGGACTC ACATGTCTTA TTAAAGTGGG AGCTGAAGCT
    GATCAAAACT ACCAAAACTA CATTCTCAGA GCACTTGGAC AGATCATGCT GTATGTAGAT
    GGAATGAATG GGCTTATCGG ACACAATGAA ACCGTACAGT GGCTGTACAC ACTGGTTGGA
    TCAAAGTTCC GGTTGGTTGT GAAAACTGCT ATGAAGCTGC TGCTGGTTTT TGTGGAGTAC
    ACCGAGAGCA ACGCACCTCT CCTCATCCAA GCCGTCAACA GCGTTGACAC CAAACAAGGC
    TCTAAGCCCT GGTCTAATGC CATGGAAATC CTTGACGAGA AAGATGGAAT GGACACTGAG
    CTGCTGGTTT ATGCCATGAC ACTAATCAAC AAGACATTAG CTGGTCTGCC TGACCAGGAC
    TCCTTCTATG ATGTGGTTGA CAGTCTGGAG GAGCAGAACA TGGAGGCTGT TGCCCAAAGA
    CACATTAGTA GAAAGGGCAC AGATCTGGAC TTGGTAGAGC AGTTCAACAT TTATGAGGCA
    ACTCTTCGTC ATGAAGACGG TGATGATGAA GCTCAGTCTC CTCCATCTGG ACATAAATGT
    CGGCGTCGGG CTAGTGTTGG AGCAACACCT GAGAGACGGG GTTTGGAACG ACGGCGTAGC
    CGAAGACATT CACTCCAGAA CGGAAAAGGC CACACTTCTG CTCCAACTTC CCCCTGTCAC
    AGCCATACAC CCAACATCCT GTCCTTTAAC AGCCAGAGGA TAGAAGACAT CAGTGAAAGA
    GAGGATGAGG AAGAGGAGGA GGAAAAGGAT GAAGAGTTTC AGAAAGCAGC ACTTTCTAGA
    CAGGCATCTG GAAGCTGTGT GGCATCAACA GTAGAGGACG CTTCTAGCTT GTGTTTGCCT
    TTAAGGTGCA TGGTCAACTT CATTCCAAAT GCTGCTGACA CAGAAAATGC AGAAAAACCT
    CGGAACAGGT CTGGGATCAG GGTTATCCAC AAGGCCCCAA TGTCCTCTCC TCCTTTTACC
    CCCGATTTTG TCCCCCCGTT ATCACCCAGA CTGTCTTCTT GGTCTTCAGT CAGATCTTTA
    GGAGCCGTCC CATCCCAGGG TTCCTCTGTC ACCTCCTCAG CCTCCACTTC CCCGGGCGAA
    GAGCCCCAAT CCCCTGTCCT CCCTCTTCTC TCTTCCAACC TTCTTCATAC TTGCCCAGAG
    GATTTGGACG AGGTGTTTGT AGGCACTCTA CAGGGTCAAG ATCCAGACAA ACACTGCAGT
    TTGTCAGATG TTAGTTACCC AAACACACCA TGCAGTTCTT GTGGCAGCAA TGGCAATGCA
    AGCACATATC CCGGCAGGTC ATCTGGGGTG TCCAGTGCCC TCTCCACACC CTACAACCGC
    TTCGGTCCTT CAGGAGGTCC ATCTGCAGAC AGTAAAGCCG ACAGGCCAAC TCTGGGTGGA
    TTGTTGACCT CCTCCTACAG GCAGCACCAA GAATCTCTGA ACGCTGAAAG AGAGCGGAGA
    CGTCTGGAGA GAGAAGAGAG ACTTCAACGG ATTGAAAGAG AGGAGAGAAA CCGCTTCAAT
    CGGGATTATG CAGACAAGAG AGAAGAGGTC AGACATGCCC GTGAAGAAAG ATACAAGCAA
    GTGGAGCGTC TGACAGCAGA GGAAAAAGAA AGGGAACGGC CGAGAACACG TGGTAGAACA
    GAAATCACCC TCAGTCTGAG TTCACGCACT TCCAGCAGCT CCTTGTCTCG TTCCGTCACC
    CCTTTGACTG TAGATGCTCA AGAGGAGACA CGCCCTGTCT CACATACAGC ACAAGGAACT
    GTGACTGAGA AAAGTTGTAC GGTGCAGACA GCAGTTGTGC GTTCTTTATC AGAACCTCCC
    TCTGCATCTC CACCAGTGTC CCCATCGCAA CCAGAGGAGC AACAGAGACC TGTCGACCCA
    TCCGACATCA TTCTATCTCT ATCTCCAACA AACTCTGAAC CAGAAAGAAC AGAGAAAGGG
    GAGAAAAAAG AGGATGCTGA AGCAGAGGAG TGTGAGGAAA CAGTGGTGGG TGAAGTGCAA
    GATGAAAAGA AGGAAGAACC AGAAGGAAAC TTTGAGGATG AGACTAATGT GACGGGTGCA
    GAAGACTGTG AGAGGGAGAA GACGTTGGAT AGAGAAGTAG AGGAGAGTGC CATCCTAAGT
    GAAAAGGAGA AGCAGAATGA AGAACTGAAT GAGAAAGATA ACTGCTCTGC CTCCAGCATC
    TCTTCAGCCA GCAGCACTCT GGAGAGAGAA GAAAGAGAGG AGAAACATGT CAGTGACAGC
    AACTCAGGTC AATGGGCAGA CTGTATAAAT ACAGCAGACA ACAACGATCA GTGCAACAAA
    GTTCTTAACA GCAAACGTTT CATGCTGGAC ATGCTGTATG CCCAAAACAA GGAAGGAGAG
    GAAGATGAGA AAGAAAAGGA GAACGAAAAT GAGAAATGTT TAGGTCAGGA CTCTACTGTG
    ACAAGCCTTG CTAGCCGAAT TTCCACTCTG GAATCACATC GGCAGACATG TGAGGACAAC
    TTGAAGAAAC TGGAAGTGGA GCATCTGGAA AACCAGGGCA GCGTGCGGGC TGTAGCTGAG
    AAATTTGGAG ATTTGGTCAA AGGCATGTTA TCACCTCATG ACTTGGAGAA TTGTGGGAAG
    GAAAAGCAGC AAACACAGGA AAAGCCTCCA CCTGCTCCAT CACCAGCACC CCCAAAAAAG
    GAGTCGGACC ACATATGGGA CCAGCTAATG GCAACGCCAC GGGAGCTTCG TATCAAAGAG
    CTGGATTTCA CAGACTTGGG AGAGGAGGAC GATGTGGATA TTTTGGACGT GGGGAACATG
    ATTGGGTCAG GTGACCTCAC TCCACCTCCT CCACCACCGC CCTGCTTGCC CAACCTTCCT
    CCCCCGCCAC CTTTATTTGG ATGCCCTCTG CCTCCCCCAG TCCCTGGCAT GATGATGCCC
    CCTCCACCAC CCTTTTTGGC GTCAAAACCT CCAGCACAAT TGGGATCTCC TCAGCTTAGT
    CGAGGAGAAC CACCGCTCTT CCAAAAGAAG AAGAAGACCA TCAGGCTATT CTGGAATGAG
    GTGAGACCGA TTGACTGGCA GTACACCAAC CACAAGAGAT GCAAGGAGTC ACTCTGGTCC
    AAACTGGATC CCATCAAACT GGACACAGCC AAATTGGAAA ACCTCTTTGA GACGAAATCT
    AAGGAGCTGC CAGTTACAAA GAAAACAGCA GCAGATGGTA AGCGTCAGGA GATCATCATT
    CTGGATTCCA AAAGAAGTAA TGCTATCAAC ATTGGCCTGA CGGTCCTGCC GCCTCCTCGC
    ACCATCAAAA CAGCCATCCT CAACTTTGAT GAGTACGCCT TGAGCAAAGA GGGCATAGAG
    AAAATCCTCA CCATGATTCC TACGGAGGAG GAGACACAGA AGATTCAGGA GGCCCAGCTG
    GCGAACCCCG ACACTCCGCT GGGCAGCGCA GAGCAGTTCC TCCTCATTTT GTCCTCCATC
    AGTGAGCTGT CCGCCCGCCT GCAACTCTGG GCCTTTAAGA TGGACTATGA TGCTTTAGAG
    AAGGAAGTAG CAGAACCACT ACAGGACCTA AAAGAGGGCA TGGACCAATT AGAAAAGAAT
    AAAACGTTAC GCTCTATCCT TTCGACTCTG CTAGCTATTG GCAACTTTCT CAATGGGACC
    AATGCTAAAG GCTTTGAGTT GAGTTATCTA GAGAAAGTTC CTGAAGTGAA GGACACGGTT
    CACAAACAGT CTCTACTGCA TCATGTCTGC TCGATTGTTG TGGAGAAGTT TCCAGAGAGC
    ACAGATCTTT ACTCTGAGAT TGGAGCCATT ACTCGCTCTG CTAAGGTTGA CTTTGATCAG
    CTTCAGGAAA ACCTGTGTCA AATGGAACGG CGCTGCAAAG CATCCTGGGA CCACCTGAAG
    GTCATCTCCA AACACGAGAT TAAACCAGCC CTTAAACAGC GAATGTCCGA CTTCCTCAAA
    GACTGTGCAG GAAGAATTAT CATCCTTAAA ATTGTCCATC GTCGTATCAT AAACAGGTTT
    CACTCATTCC TGCTATTTCT GGGCCACCCA CCATACGCCA TTAGAGAAGT GAGCGTCCAC
    CGCTTCAGCA AGATTCTGAG TGAGTTTGCT CTTGAGTACC GAACCACCAG AGAGCGAGTG
    CTGCAGCAGA AACAGAAACG AGCCAATCAT CGTGAGAGGA ACAAGACCCG CGGCAAAATG
    ATCACAGAGA GTGATGAGGA AGAGGAGGAT GAGAGTGGGA AGTTTGCAGG AAGTCCTATG
    GAGGGTCAGG AGTGTCAACC GCAGGGTTTG GGCTACACAG AGGACGTGAC TGAGCATGAG
    CAGATGACCG CAGTGCTTTG TACCAGCCTG CAGGGTGGCG ATAGTCCATC ATCTGTCCTT
    CCTGGCCTTC GCACACGGAC CCGAGCCACT CGAGGTCATA CAGCACTTTT GTCCACTGCA
    AATGAAGACA CTTCTAATTG CACAAATGAC ACAGCAGATG AGATCATGGA GCGGATTGTG
    AAATCAGCCA CTCAGGGCCC CAGTCAGAGA ACACAGCCTC GTGAGAGGAG ACGTTCCAGA
    GCAAACCGCA AATCCTTGAG AAGAACACTG AAGAACGGTC TTACCCCGGA GGAGGCACAG
    GCTCTGGGTT TGGCCAGCGG CTCTGAGATG CAGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322566.1
    CDS657..5735
    Translation

    Target ORF information:

    RefSeq Version XM_021466891.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio formin homology 2 domain containing 3b (fhod3b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466891.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGCTACGT TTGCATGCAG GGTGCAGTTC TTAGATGATA CAGATCCATT TAATAGCACA 
    AATTTTCCGG AGCCGACCAG ACCCCCACAT TTCACATTCA GAGAGGATAT TCCGCTCATC
    AATCAGATCG CCGGAGTTCA CAGACTTCTC AAAGCACCTC ACAAGCTTGA TGACTGTGCT
    TTGCAGTTGT CTCATAATGG CAGTTATCTG GACCTGGAGT CCACCTTGGC TGAGCAGAAA
    GATGAACTTG AAGGCTTTCA GGAGGATGGA GGGAGAGGAA AGAAGCACAG TATTGTACTT
    AGAACACAGC TGTCTGTCAG AGTCCATGCC TGCATAGAAA AGCTGTACAA CTCGACGGGT
    CGTGAGCTAC GAAGAGCACT GTTCTCTCTC AAGCAAATCT TCCAGGATGA TAAGGATCTG
    GTTCATGAGT TTGTGATGGC GGAGGGACTC ACATGTCTTA TTAAAGTGGG AGCTGAAGCT
    GATCAAAACT ACCAAAACTA CATTCTCAGA GCACTTGGAC AGATCATGCT GTATGTAGAT
    GGAATGAATG GGCTTATCGG ACACAATGAA ACCGTACAGT GGCTGTACAC ACTGGTTGGA
    TCAAAGTTCC GGTTGGTTGT GAAAACTGCT ATGAAGCTGC TGCTGGTTTT TGTGGAGTAC
    ACCGAGAGCA ACGCACCTCT CCTCATCCAA GCCGTCAACA GCGTTGACAC CAAACAAGGC
    TCTAAGCCCT GGTCTAATGC CATGGAAATC CTTGACGAGA AAGATGGAAT GGACACTGAG
    CTGCTGGTTT ATGCCATGAC ACTAATCAAC AAGACATTAG CTGGTCTGCC TGACCAGGAC
    TCCTTCTATG ATGTGGTTGA CAGTCTGGAG GAGCAGAACA TGGAGGCTGT TGCCCAAAGA
    CACATTAGTA GAAAGGGCAC AGATCTGGAC TTGGTAGAGC AGTTCAACAT TTATGAGGCA
    ACTCTTCGTC ATGAAGACGG TGATGATGAA GCTCAGTCTC CTCCATCTGG ACATAAATGT
    CGGCGTCGGG CTAGTGTTGG AGCAACACCT GAGAGACGGG GTTTGGAACG ACGGCGTAGC
    CGAAGACATT CACTCCAGAA CGGAAAAGGC CACACTTCTG CTCCAACTTC CCCCTGTCAC
    AGCCATACAC CCAACATCCT GTCCTTTAAC AGCCAGAGGA TAGAAGACAT CAGTGAAAGA
    GAGGATGAGG AAGAGGAGGA GGAAAAGGAT GAAGAGTTTC AGAAAGCAGC ACTTTCTAGA
    CAGGCATCTG GAAGCTGTGT GGCATCAACA GTAGAGGACG CTTCTAGCTT GTGTTTGCCT
    TTAAGGTGCA TGGTCAACTT CATTCCAAAT GCTGCTGACA CAGAAAATGC AGAAAAACCT
    CGGAACAGGT CTGGGATCAG GGTTATCCAC AAGGCCCCAA TGTCCTCTCC TCCTTTTACC
    CCCGATTTTG TCCCCCCGTT ATCACCCAGA CTGTCTTCTT GGTCTTCAGT CAGATCTTTA
    GGAGCCGTCC CATCCCAGGG TTCCTCTGTC ACCTCCTCAG CCTCCACTTC CCCGGGCGAA
    GAGCCCCAAT CCCCTGTCCT CCCTCTTCTC TCTTCCAACC TTCTTCATAC TTGCCCAGAG
    GATTTGGACG AGGTGTTTGT AGGCACTCTA CAGGGTCAAG ATCCAGACAA ACACTGCAGT
    TTGTCAGATG TTAGTTACCC AAACACACCA TGCAGTTCTT GTGGCAGCAA TGGCAATGCA
    AGCACATATC CCGGCAGGTC ATCTGGGGTG TCCAGTGCCC TCTCCACACC CTACAACCGC
    TTCGGTCCTT CAGGAGGTCC ATCTGCAGAC AGTAAAGCCG ACAGGCCAAC TCTGGGTGGA
    TTGTTGACCT CCTCCTACAG GCAGCACCAA GAATCTCTGA ACGCTGAAAG AGAGCGGAGA
    CGTCTGGAGA GAGAAGAGAG ACTTCAACGG ATTGAAAGAG AGGAGAGAAA CCGCTTCAAT
    CGGGATTATG CAGACAAGAG AGAAGAGGTC AGACATGCCC GTGAAGAAAG ATACAAGCAA
    GTGGAGCGTC TGACAGCAGA GGAAAAAGAA AGGGAACGGC CGAGAACACG TGGTAGAACA
    GAAATCACCC TCAGTCTGAG TTCACGCACT TCCAGCAGCT CCTTGTCTCG TTCCGTCACC
    CCTTTGACTG TAGATGCTCA AGAGGAGACA CGCCCTGTCT CACATACAGC ACAAGGAACT
    GTGACTGAGA AAAGTTGTAC GGTGCAGACA GCAGTTGTGC GTTCTTTATC AGAACCTCCC
    TCTGCATCTC CACCAGTGTC CCCATCGCAA CCAGAGGAGC AACAGAGACC TGTCGACCCA
    TCCGACATCA TTCTATCTCT ATCTCCAACA AACTCTGAAC CAGAAAGAAC AGAGAAAGGG
    GAGAAAAAAG AGGATGCTGA AGCAGAGGAG TGTGAGGAAA CAGTGGTGGG TGAAGTGCAA
    GATGAAAAGA AGGAAGAACC AGAAGGAAAC TTTGAGGATG AGACTAATGT GACGGGTGCA
    GAAGACTGTG AGAGGGAGAA GACGTTGGAT AGAGAAGTAG AGGAGAGTGC CATCCTAAGT
    GAAAAGGAGA AGCAGAATGA AGAACTGAAT GAGAAAGATA ACTGCTCTGC CTCCAGCATC
    TCTTCAGCCA GCAGCACTCT GGAGAGAGAA GAAAGAGAGG AGAAACATGT CAGTGACAGC
    AACTCAGGTC AATGGGCAGA CTGTATAAAT ACAGCAGACA ACAACGATCA GTGCAACAAA
    GTTCTTAACA GCAAACGTTT CATGCTGGAC ATGCTGTATG CCCAAAACAA GGAAGGAGAG
    GAAGATGAGA AAGAAAAGGA GAACGAAAAT GAGAAATGTT TAGGTCAGGA CTCTACTGTG
    ACAAGCCTTG CTAGCCGAAT TTCCACTCTG GAATCACATC GGCAGACATG TGAGGACAAC
    TTGAAGAAAC TGGAAGTGGA GCATCTGGAA AACCAGGGCA GCGTGCGGGC TGTAGCTGAG
    AAATTTGGAG ATTTGGTCAA AGGCATGTTA TCACCTCATG ACTTGGAGAA TTGTGGGAAG
    GAAAAGCAGC AAACACAGGA AAAGCCTCCA CCTGCTCCAT CACCAGCACC CCCAAAAAAG
    GAGTCGGACC ACATATGGGA CCAGCTAATG GCAACGCCAC GGGAGCTTCG TATCAAAGAG
    CTGGATTTCA CAGACTTGGG AGAGGAGGAC GATGTGGATA TTTTGGACGT GGGGAACATG
    ATTGGGTCAG GTGACCTCAC TCCACCTCCT CCACCACCGC CCTGCTTGCC CAACCTTCCT
    CCCCCGCCAC CTTTATTTGG ATGCCCTCTG CCTCCCCCAG TCCCTGGCAT GATGATGCCC
    CCTCCACCAC CCTTTTTGGC GTCAAAACCT CCAGCACAAT TGGGATCTCC TCAGCTTAGT
    CGAGGAGAAC CACCGCTCTT CCAAAAGAAG AAGAAGACCA TCAGGCTATT CTGGAATGAG
    GTGAGACCGA TTGACTGGCA GTACACCAAC CACAAGAGAT GCAAGGAGTC ACTCTGGTCC
    AAACTGGATC CCATCAAACT GGACACAGCC AAATTGGAAA ACCTCTTTGA GACGAAATCT
    AAGGAGCTGC CAGTTACAAA GAAAACAGCA GCAGATGGTA AGCGTCAGGA GATCATCATT
    CTGGATTCCA AAAGAAGTAA TGCTATCAAC ATTGGCCTGA CGGTCCTGCC GCCTCCTCGC
    ACCATCAAAA CAGCCATCCT CAACTTTGAT GAGTACGCCT TGAGCAAAGA GGGCATAGAG
    AAAATCCTCA CCATGATTCC TACGGAGGAG GAGACACAGA AGATTCAGGA GGCCCAGCTG
    GCGAACCCCG ACACTCCGCT GGGCAGCGCA GAGCAGTTCC TCCTCATTTT GTCCTCCATC
    AGTGAGCTGT CCGCCCGCCT GCAACTCTGG GCCTTTAAGA TGGACTATGA TGCTTTAGAG
    AAGGAAGTAG CAGAACCACT ACAGGACCTA AAAGAGGGCA TGGACCAATT AGAAAAGAAT
    AAAACGTTAC GCTCTATCCT TTCGACTCTG CTAGCTATTG GCAACTTTCT CAATGGGACC
    AATGCTAAAG GCTTTGAGTT GAGTTATCTA GAGAAAGTTC CTGAAGTGAA GGACACGGTT
    CACAAACAGT CTCTACTGCA TCATGTCTGC TCGATTGTTG TGGAGAAGTT TCCAGAGAGC
    ACAGATCTTT ACTCTGAGAT TGGAGCCATT ACTCGCTCTG CTAAGGTTGA CTTTGATCAG
    CTTCAGGAAA ACCTGTGTCA AATGGAACGG CGCTGCAAAG CATCCTGGGA CCACCTGAAG
    GTCATCTCCA AACACGAGAT TAAACCAGCC CTTAAACAGC GAATGTCCGA CTTCCTCAAA
    GACTGTGCAG GAAGAATTAT CATCCTTAAA ATTGTCCATC GTCGTATCAT AAACAGGTTT
    CACTCATTCC TGCTATTTCT GGGCCACCCA CCATACGCCA TTAGAGAAGT GAGCGTCCAC
    CGCTTCAGCA AGATTCTGAG TGAGTTTGCT CTTGAGTACC GAACCACCAG AGAGCGAGTG
    CTGCAGCAGA AACAGAAACG AGCCAATCAT CGTGAGAGGA ACAAGACCCG CGGCAAAATG
    ATCACAGAGA GTGATGAGGA AGAGGAGGAT GAGAGTGGGA AGTTTGCAGG AAGTCCTATG
    GAGGGTCAGG AGTGTCAACC GCAGGGTTTG GGCTACACAG AGGACGTGAC TGAGCATGAG
    CAGATGACCG CAGTGCTTTG TACCAGCCTG CAGGGTGGCG ATAGTCCATC ATCTGTCCTT
    CCTGGCCTTC GCACACGGAC CCGAGCCACT CGAGGTCATA CAGCACTTTT GTCCACTGCA
    AATGAAGACA CTTCTAATTG CACAAATGAC ACAGCAGATG AGATCATGGA GCGGATTGTG
    AAATCAGCCA CTCAGGGCCC CAGTCAGAGA ACACAGCCTC GTGAGAGGAG ACGTTCCAGA
    GCAAACCGCA AATCCTTGAG AAGAACACTG AAGAACGGTC TTACCCCGGA GGAGGCACAG
    GCTCTGGGTT TGGCCAGCGG CTCTGAGATG CAGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149377
    Clone ID Related Accession (Same CDS sequence) XM_021466892.1
    Accession Version XM_021466892.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4674bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product FH1/FH2 domain-containing protein 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTACGT TTGCATGCAG GGTGCAGTTC TTAGATGATA CAGATCCATT TAATAGCACA 
    AATTTTCCGG AGCCGACCAG ACCCCCACAT TTCACATTCA GAGAGGATAT TCCGCTCATC
    AATCAGATCG CCGGAGTTCA CAGACTTCTC AAAGCACCTC ACAAGCTTGA TGACTGTGCT
    TTGCAGTTGT CTCATAATGG CAGTTATCTG GACCTGGAGT CCACCTTGGC TGAGCAGAAA
    GATGAACTTG AAGGCTTTCA GGAGGATGGA GGGAGAGGAA AGAAGCACAG TATTGTACTT
    AGAACACAGC TGTCTGTCAG AGTCCATGCC TGCATAGAAA AGCTGTACAA CTCGACGGGT
    CGTGAGCTAC GAAGAGCACT GTTCTCTCTC AAGCAAATCT TCCAGGATGA TAAGGATCTG
    GTTCATGAGT TTGTGATGGC GGAGGGACTC ACATGTCTTA TTAAAGTGGG AGCTGAAGCT
    GATCAAAACT ACCAAAACTA CATTCTCAGA GCACTTGGAC AGATCATGCT GTATGTAGAT
    GGAATGAATG GGCTTATCGG ACACAATGAA ACCGTACAGT GGCTGTACAC ACTGGTTGGA
    TCAAAGTTCC GGTTGGTTGT GAAAACTGCT ATGAAGCTGC TGCTGGTTTT TGTGGAGTAC
    ACCGAGAGCA ACGCACCTCT CCTCATCCAA GCCGTCAACA GCGTTGACAC CAAACAAGGC
    TCTAAGCCCT GGTCTAATGC CATGGAAATC CTTGACGAGA AAGATGGAAT GGACACTGAG
    CTGCTGGTTT ATGCCATGAC ACTAATCAAC AAGACATTAG CTGGTCTGCC TGACCAGGAC
    TCCTTCTATG ATGTGGTTGA CAGTCTGGAG GAGCAGAACA TGGAGGCTGT TGCCCAAAGA
    CACATTAGTA GAAAGGGCAC AGATCTGGAC TTGGTAGAGC AGTTCAACAT TTATGAGGCA
    ACTCTTCGTC ATGAAGACGG TGATGATGAA GCTCAGTCTC CTCCATCTGG ACATAAATGT
    CGGCGTCGGG CTAGTGTTGG AGCAACACCT GAGAGACGGG GTTTGGAACG ACGGCGTAGC
    CGAAGACATT CACTCCAGAA CGGAAAAGGC CACACTTCTG CTCCAACTTC CCCCTGTCAC
    AGCCATACAC CCAACATCCT GTCCTTTAAC AGCCAGAGGA TAGAAGACAT CAGTGAAAGA
    GAGGATGAGG AAGAGGAGGA GGAAAAGGAT GAAGAGTTTC AGAAAGCAGC ACTTTCTAGA
    CAGGCATCTG GAAGTTTGTC AGATGTTAGT TACCCAAACA CACCATGCAG TTCTTGTGGC
    AGCAATGGCA ATGCAAGCAC ATATCCCGGC AGGTCATCTG GGGTGTCCAG TGCCCTCTCC
    ACACCCTACA ACCGCTTCGG TCCTTCAGGA GGTCCATCTG CAGACAGTAA AGCCGACAGG
    CCAACTCTGG GTGGATTGTT GACCTCCTCC TACAGGCAGC ACCAAGAATC TCTGAACGCT
    GAAAGAGAGC GGAGACGTCT GGAGAGAGAA GAGAGACTTC AACGGATTGA AAGAGAGGAG
    AGAAACCGCT TCAATCGGGA TTATGCAGAC AAGAGAGAAG AGGTCAGACA TGCCCGTGAA
    GAAAGATACA AGCAAGTGGA GCGTCTGACA GCAGAGGAAA AAGAAAGGGA ACGGCCGAGA
    ACACGTGGTA GAACAGAAAT CACCCTCAGT CTGAGTTCAC GCACTTCCAG CAGCTCCTTG
    TCTCGTTCCG TCACCCCTTT GACTGTAGAT GCTCAAGAGG AGACACGCCC TGTCTCACAT
    ACAGCACAAG GAACTGTGAC TGAGAAAAGT TGTACGGTGC AGACAGCAGT TGTGCGTTCT
    TTATCAGAAC CTCCCTCTGC ATCTCCACCA GTGTCCCCAT CGCAACCAGA GGAGCAACAG
    AGACCTGTCG ACCCATCCGA CATCATTCTA TCTCTATCTC CAACAAACTC TGAACCAGAA
    AGAACAGAGA AAGGGGAGAA AAAAGAGGAT GCTGAAGCAG AGGAGTGTGA GGAAACAGTG
    GTGGGTGAAG TGCAAGATGA AAAGAAGGAA GAACCAGAAG GAAACTTTGA GGATGAGACT
    AATGTGACGG GTGCAGAAGA CTGTGAGAGG GAGAAGACGT TGGATAGAGA AGTAGAGGAG
    AGTGCCATCC TAAGTGAAAA GGAGAAGCAG AATGAAGAAC TGAATGAGAA AGATAACTGC
    TCTGCCTCCA GCATCTCTTC AGCCAGCAGC ACTCTGGAGA GAGAAGAAAG AGAGGAGAAA
    CATGTCAGTG ACAGCAACTC AGGTCAATGG GCAGACTGTA TAAATACAGC AGACAACAAC
    GATCAGTGCA ACAAAGTTCT TAACAGCAAA CGTTTCATGC TGGACATGCT GTATGCCCAA
    AACAAGGAAG GAGAGGAAGA TGAGAAAGAA AAGGAGAACG AAAATGAGAA ATGTTTAGGT
    CAGGACTCTA CTGTGACAAG CCTTGCTAGC CGAATTTCCA CTCTGGAATC ACATCGGCAG
    ACATGTGAGG ACAACTTGAA GAAACTGGAA GTGGAGCATC TGGAAAACCA GGGCAGCGTG
    CGGGCTGTAG CTGAGAAATT TGGAGATTTG GTCAAAGGCA TGTTATCACC TCATGACTTG
    GAGAATTGTG GGAAGGAAAA GCAGCAAACA CAGGAAAAGC CTCCACCTGC TCCATCACCA
    GCACCCCCAA AAAAGGAGTC GGACCACATA TGGGACCAGC TAATGGCAAC GCCACGGGAG
    CTTCGTATCA AAGAGCTGGA TTTCACAGAC TTGGGAGAGG AGGACGATGT GGATATTTTG
    GACGTGGGGA ACATGATTGG GTCAGGTGAC CTCACTCCAC CTCCTCCACC ACCGCCCTGC
    TTGCCCAACC TTCCTCCCCC GCCACCTTTA TTTGGATGCC CTCTGCCTCC CCCAGTCCCT
    GGCATGATGA TGCCCCCTCC ACCACCCTTT TTGGCGTCAA AACCTCCAGC ACAATTGGGA
    TCTCCTCAGC TTAGTCGAGG AGAACCACCG CTCTTCCAAA AGAAGAAGAA GACCATCAGG
    CTATTCTGGA ATGAGGTGAG ACCGATTGAC TGGCAGTACA CCAACCACAA GAGATGCAAG
    GAGTCACTCT GGTCCAAACT GGATCCCATC AAACTGGACA CAGCCAAATT GGAAAACCTC
    TTTGAGACGA AATCTAAGGA GCTGCCAGTT ACAAAGAAAA CAGCAGCAGA TGGTAAGCGT
    CAGGAGATCA TCATTCTGGA TTCCAAAAGA AGTAATGCTA TCAACATTGG CCTGACGGTC
    CTGCCGCCTC CTCGCACCAT CAAAACAGCC ATCCTCAACT TTGATGAGTA CGCCTTGAGC
    AAAGAGGGCA TAGAGAAAAT CCTCACCATG ATTCCTACGG AGGAGGAGAC ACAGAAGATT
    CAGGAGGCCC AGCTGGCGAA CCCCGACACT CCGCTGGGCA GCGCAGAGCA GTTCCTCCTC
    ATTTTGTCCT CCATCAGTGA GCTGTCCGCC CGCCTGCAAC TCTGGGCCTT TAAGATGGAC
    TATGATGCTT TAGAGAAGGA AGTAGCAGAA CCACTACAGG ACCTAAAAGA GGGCATGGAC
    CAATTAGAAA AGAATAAAAC GTTACGCTCT ATCCTTTCGA CTCTGCTAGC TATTGGCAAC
    TTTCTCAATG GGACCAATGC TAAAGGCTTT GAGTTGAGTT ATCTAGAGAA AGTTCCTGAA
    GTGAAGGACA CGGTTCACAA ACAGTCTCTA CTGCATCATG TCTGCTCGAT TGTTGTGGAG
    AAGTTTCCAG AGAGCACAGA TCTTTACTCT GAGATTGGAG CCATTACTCG CTCTGCTAAG
    GTTGACTTTG ATCAGCTTCA GGAAAACCTG TGTCAAATGG AACGGCGCTG CAAAGCATCC
    TGGGACCACC TGAAGGTCAT CTCCAAACAC GAGATTAAAC CAGCCCTTAA ACAGCGAATG
    TCCGACTTCC TCAAAGACTG TGCAGGAAGA ATTATCATCC TTAAAATTGT CCATCGTCGT
    ATCATAAACA GGTTTCACTC ATTCCTGCTA TTTCTGGGCC ACCCACCATA CGCCATTAGA
    GAAGTGAGCG TCCACCGCTT CAGCAAGATT CTGAGTGAGT TTGCTCTTGA GTACCGAACC
    ACCAGAGAGC GAGTGCTGCA GCAGAAACAG AAACGAGCCA ATCATCGTGA GAGGAACAAG
    ACCCGCGGCA AAATGATCAC AGAGAGTGAT GAGGAAGAGG AGGATGAGAG TGGGAAGTTT
    GCAGGAAGTC CTATGGAGGG TCAGGAGTGT CAACCGCAGG GTTTGGGCTA CACAGAGGAC
    GTGACTGAGC ATGAGCAGAT GACCGCAGTG CTTTGTACCA GCCTGCAGGG TGGCGATAGT
    CCATCATCTG TCCTTCCTGG CCTTCGCACA CGGACCCGAG CCACTCGAGG TCATACAGCA
    CTTTTGTCCA CTGCAAATGA AGACACTTCT AATTGCACAA ATGACACAGC AGATGAGATC
    ATGGAGCGGA TTGTGAAATC AGCCACTCAG GGCCCCAGTC AGAGAACACA GCCTCGTGAG
    AGGAGACGTT CCAGAGCAAA CCGCAAATCC TTGAGAAGAA CACTGAAGAA CGGTCTTACC
    CCGGAGGAGG CACAGGCTCT GGGTTTGGCC AGCGGCTCTG AGATGCAGGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322567.1
    CDS654..5327
    Translation

    Target ORF information:

    RefSeq Version XM_021466892.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio formin homology 2 domain containing 3b (fhod3b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466892.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTACGT TTGCATGCAG GGTGCAGTTC TTAGATGATA CAGATCCATT TAATAGCACA 
    AATTTTCCGG AGCCGACCAG ACCCCCACAT TTCACATTCA GAGAGGATAT TCCGCTCATC
    AATCAGATCG CCGGAGTTCA CAGACTTCTC AAAGCACCTC ACAAGCTTGA TGACTGTGCT
    TTGCAGTTGT CTCATAATGG CAGTTATCTG GACCTGGAGT CCACCTTGGC TGAGCAGAAA
    GATGAACTTG AAGGCTTTCA GGAGGATGGA GGGAGAGGAA AGAAGCACAG TATTGTACTT
    AGAACACAGC TGTCTGTCAG AGTCCATGCC TGCATAGAAA AGCTGTACAA CTCGACGGGT
    CGTGAGCTAC GAAGAGCACT GTTCTCTCTC AAGCAAATCT TCCAGGATGA TAAGGATCTG
    GTTCATGAGT TTGTGATGGC GGAGGGACTC ACATGTCTTA TTAAAGTGGG AGCTGAAGCT
    GATCAAAACT ACCAAAACTA CATTCTCAGA GCACTTGGAC AGATCATGCT GTATGTAGAT
    GGAATGAATG GGCTTATCGG ACACAATGAA ACCGTACAGT GGCTGTACAC ACTGGTTGGA
    TCAAAGTTCC GGTTGGTTGT GAAAACTGCT ATGAAGCTGC TGCTGGTTTT TGTGGAGTAC
    ACCGAGAGCA ACGCACCTCT CCTCATCCAA GCCGTCAACA GCGTTGACAC CAAACAAGGC
    TCTAAGCCCT GGTCTAATGC CATGGAAATC CTTGACGAGA AAGATGGAAT GGACACTGAG
    CTGCTGGTTT ATGCCATGAC ACTAATCAAC AAGACATTAG CTGGTCTGCC TGACCAGGAC
    TCCTTCTATG ATGTGGTTGA CAGTCTGGAG GAGCAGAACA TGGAGGCTGT TGCCCAAAGA
    CACATTAGTA GAAAGGGCAC AGATCTGGAC TTGGTAGAGC AGTTCAACAT TTATGAGGCA
    ACTCTTCGTC ATGAAGACGG TGATGATGAA GCTCAGTCTC CTCCATCTGG ACATAAATGT
    CGGCGTCGGG CTAGTGTTGG AGCAACACCT GAGAGACGGG GTTTGGAACG ACGGCGTAGC
    CGAAGACATT CACTCCAGAA CGGAAAAGGC CACACTTCTG CTCCAACTTC CCCCTGTCAC
    AGCCATACAC CCAACATCCT GTCCTTTAAC AGCCAGAGGA TAGAAGACAT CAGTGAAAGA
    GAGGATGAGG AAGAGGAGGA GGAAAAGGAT GAAGAGTTTC AGAAAGCAGC ACTTTCTAGA
    CAGGCATCTG GAAGTTTGTC AGATGTTAGT TACCCAAACA CACCATGCAG TTCTTGTGGC
    AGCAATGGCA ATGCAAGCAC ATATCCCGGC AGGTCATCTG GGGTGTCCAG TGCCCTCTCC
    ACACCCTACA ACCGCTTCGG TCCTTCAGGA GGTCCATCTG CAGACAGTAA AGCCGACAGG
    CCAACTCTGG GTGGATTGTT GACCTCCTCC TACAGGCAGC ACCAAGAATC TCTGAACGCT
    GAAAGAGAGC GGAGACGTCT GGAGAGAGAA GAGAGACTTC AACGGATTGA AAGAGAGGAG
    AGAAACCGCT TCAATCGGGA TTATGCAGAC AAGAGAGAAG AGGTCAGACA TGCCCGTGAA
    GAAAGATACA AGCAAGTGGA GCGTCTGACA GCAGAGGAAA AAGAAAGGGA ACGGCCGAGA
    ACACGTGGTA GAACAGAAAT CACCCTCAGT CTGAGTTCAC GCACTTCCAG CAGCTCCTTG
    TCTCGTTCCG TCACCCCTTT GACTGTAGAT GCTCAAGAGG AGACACGCCC TGTCTCACAT
    ACAGCACAAG GAACTGTGAC TGAGAAAAGT TGTACGGTGC AGACAGCAGT TGTGCGTTCT
    TTATCAGAAC CTCCCTCTGC ATCTCCACCA GTGTCCCCAT CGCAACCAGA GGAGCAACAG
    AGACCTGTCG ACCCATCCGA CATCATTCTA TCTCTATCTC CAACAAACTC TGAACCAGAA
    AGAACAGAGA AAGGGGAGAA AAAAGAGGAT GCTGAAGCAG AGGAGTGTGA GGAAACAGTG
    GTGGGTGAAG TGCAAGATGA AAAGAAGGAA GAACCAGAAG GAAACTTTGA GGATGAGACT
    AATGTGACGG GTGCAGAAGA CTGTGAGAGG GAGAAGACGT TGGATAGAGA AGTAGAGGAG
    AGTGCCATCC TAAGTGAAAA GGAGAAGCAG AATGAAGAAC TGAATGAGAA AGATAACTGC
    TCTGCCTCCA GCATCTCTTC AGCCAGCAGC ACTCTGGAGA GAGAAGAAAG AGAGGAGAAA
    CATGTCAGTG ACAGCAACTC AGGTCAATGG GCAGACTGTA TAAATACAGC AGACAACAAC
    GATCAGTGCA ACAAAGTTCT TAACAGCAAA CGTTTCATGC TGGACATGCT GTATGCCCAA
    AACAAGGAAG GAGAGGAAGA TGAGAAAGAA AAGGAGAACG AAAATGAGAA ATGTTTAGGT
    CAGGACTCTA CTGTGACAAG CCTTGCTAGC CGAATTTCCA CTCTGGAATC ACATCGGCAG
    ACATGTGAGG ACAACTTGAA GAAACTGGAA GTGGAGCATC TGGAAAACCA GGGCAGCGTG
    CGGGCTGTAG CTGAGAAATT TGGAGATTTG GTCAAAGGCA TGTTATCACC TCATGACTTG
    GAGAATTGTG GGAAGGAAAA GCAGCAAACA CAGGAAAAGC CTCCACCTGC TCCATCACCA
    GCACCCCCAA AAAAGGAGTC GGACCACATA TGGGACCAGC TAATGGCAAC GCCACGGGAG
    CTTCGTATCA AAGAGCTGGA TTTCACAGAC TTGGGAGAGG AGGACGATGT GGATATTTTG
    GACGTGGGGA ACATGATTGG GTCAGGTGAC CTCACTCCAC CTCCTCCACC ACCGCCCTGC
    TTGCCCAACC TTCCTCCCCC GCCACCTTTA TTTGGATGCC CTCTGCCTCC CCCAGTCCCT
    GGCATGATGA TGCCCCCTCC ACCACCCTTT TTGGCGTCAA AACCTCCAGC ACAATTGGGA
    TCTCCTCAGC TTAGTCGAGG AGAACCACCG CTCTTCCAAA AGAAGAAGAA GACCATCAGG
    CTATTCTGGA ATGAGGTGAG ACCGATTGAC TGGCAGTACA CCAACCACAA GAGATGCAAG
    GAGTCACTCT GGTCCAAACT GGATCCCATC AAACTGGACA CAGCCAAATT GGAAAACCTC
    TTTGAGACGA AATCTAAGGA GCTGCCAGTT ACAAAGAAAA CAGCAGCAGA TGGTAAGCGT
    CAGGAGATCA TCATTCTGGA TTCCAAAAGA AGTAATGCTA TCAACATTGG CCTGACGGTC
    CTGCCGCCTC CTCGCACCAT CAAAACAGCC ATCCTCAACT TTGATGAGTA CGCCTTGAGC
    AAAGAGGGCA TAGAGAAAAT CCTCACCATG ATTCCTACGG AGGAGGAGAC ACAGAAGATT
    CAGGAGGCCC AGCTGGCGAA CCCCGACACT CCGCTGGGCA GCGCAGAGCA GTTCCTCCTC
    ATTTTGTCCT CCATCAGTGA GCTGTCCGCC CGCCTGCAAC TCTGGGCCTT TAAGATGGAC
    TATGATGCTT TAGAGAAGGA AGTAGCAGAA CCACTACAGG ACCTAAAAGA GGGCATGGAC
    CAATTAGAAA AGAATAAAAC GTTACGCTCT ATCCTTTCGA CTCTGCTAGC TATTGGCAAC
    TTTCTCAATG GGACCAATGC TAAAGGCTTT GAGTTGAGTT ATCTAGAGAA AGTTCCTGAA
    GTGAAGGACA CGGTTCACAA ACAGTCTCTA CTGCATCATG TCTGCTCGAT TGTTGTGGAG
    AAGTTTCCAG AGAGCACAGA TCTTTACTCT GAGATTGGAG CCATTACTCG CTCTGCTAAG
    GTTGACTTTG ATCAGCTTCA GGAAAACCTG TGTCAAATGG AACGGCGCTG CAAAGCATCC
    TGGGACCACC TGAAGGTCAT CTCCAAACAC GAGATTAAAC CAGCCCTTAA ACAGCGAATG
    TCCGACTTCC TCAAAGACTG TGCAGGAAGA ATTATCATCC TTAAAATTGT CCATCGTCGT
    ATCATAAACA GGTTTCACTC ATTCCTGCTA TTTCTGGGCC ACCCACCATA CGCCATTAGA
    GAAGTGAGCG TCCACCGCTT CAGCAAGATT CTGAGTGAGT TTGCTCTTGA GTACCGAACC
    ACCAGAGAGC GAGTGCTGCA GCAGAAACAG AAACGAGCCA ATCATCGTGA GAGGAACAAG
    ACCCGCGGCA AAATGATCAC AGAGAGTGAT GAGGAAGAGG AGGATGAGAG TGGGAAGTTT
    GCAGGAAGTC CTATGGAGGG TCAGGAGTGT CAACCGCAGG GTTTGGGCTA CACAGAGGAC
    GTGACTGAGC ATGAGCAGAT GACCGCAGTG CTTTGTACCA GCCTGCAGGG TGGCGATAGT
    CCATCATCTG TCCTTCCTGG CCTTCGCACA CGGACCCGAG CCACTCGAGG TCATACAGCA
    CTTTTGTCCA CTGCAAATGA AGACACTTCT AATTGCACAA ATGACACAGC AGATGAGATC
    ATGGAGCGGA TTGTGAAATC AGCCACTCAG GGCCCCAGTC AGAGAACACA GCCTCGTGAG
    AGGAGACGTT CCAGAGCAAA CCGCAAATCC TTGAGAAGAA CACTGAAGAA CGGTCTTACC
    CCGGAGGAGG CACAGGCTCT GGGTTTGGCC AGCGGCTCTG AGATGCAGGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149378
    Clone ID Related Accession (Same CDS sequence) XM_021466893.1
    Accession Version XM_021466893.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4599bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product FH1/FH2 domain-containing protein 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCTACGT TTGCATGCAG GGTGCAGTTC TTAGATGATA CAGATCCATT TAATAGCACA 
    AATTTTCCGG AGCCGACCAG ACCCCCACAT TTCACATTCA GAGAGGATAT TCCGCTCATC
    AATCAGATCG CCGGAGTTCA CAGACTTCTC AAAGCACCTC ACAAGCTTGA TGACTGTGCT
    TTGCAGTTGT CTCATAATGG CAGTTATCTG GACCTGGAGT CCACCTTGGC TGAGCAGAAA
    GATGAACTTG AAGGCTTTCA GGAGGATGGA GGGAGAGGAA AGAAGCACAG TATTGTACTT
    AGAACACAGC TGTCTGTCAG AGTCCATGCC TGCATAGAAA AGCTGTACAA CTCGACGGGT
    CGTGAGCTAC GAAGAGCACT GTTCTCTCTC AAGCAAATCT TCCAGGATGA TAAGGATCTG
    GTTCATGAGT TTGTGATGGC GGAGGGACTC ACATGTCTTA TTAAAGTGGG AGCTGAAGCT
    GATCAAAACT ACCAAAACTA CATTCTCAGA GCACTTGGAC AGATCATGCT GTATGTAGAT
    GGAATGAATG GGCTTATCGG ACACAATGAA ACCGTACAGT GGCTGTACAC ACTGGTTGGA
    TCAAAGTTCC GGTTGGTTGT GAAAACTGCT ATGAAGCTGC TGCTGGTTTT TGTGGAGTAC
    ACCGAGAGCA ACGCACCTCT CCTCATCCAA GCCGTCAACA GCGTTGACAC CAAACAAGGC
    TCTAAGCCCT GGTCTAATGC CATGGAAATC CTTGACGAGA AAGATGGAAT GGACACTGAG
    CTGCTGGTTT ATGCCATGAC ACTAATCAAC AAGACATTAG CTGGTCTGCC TGACCAGGAC
    TCCTTCTATG ATGTGGTTGA CAGTCTGGAG GAGCAGAACA TGGAGGCTGT TGCCCAAAGA
    CACATTAGTA GAAAGGGCAC AGATCTGGAC TTGGTAGAGC AGTTCAACAT TTATGAGGCA
    ACTCTTCGTC ATGAAGACGG TGATGATGAA GCTCAGTCTC CTCCATCTGG ACATAAATGT
    CGGCGTCGGG CTAGTGTTGG AGCAACACCT GAGAGACGGG GTTTGGAACG ACGGCGTAGC
    CGAAGACATT CACTCCAGAA CGGAAAAGGC CACACTTCTG CTCCAACTTC CCCCTGTCAC
    AGCCATACAC CCAACATCCT GTCCTTTAAC AGCCAGAGGA TAGAAGACAT CAGTGAAAGT
    TTGTCAGATG TTAGTTACCC AAACACACCA TGCAGTTCTT GTGGCAGCAA TGGCAATGCA
    AGCACATATC CCGGCAGGTC ATCTGGGGTG TCCAGTGCCC TCTCCACACC CTACAACCGC
    TTCGGTCCTT CAGGAGGTCC ATCTGCAGAC AGTAAAGCCG ACAGGCCAAC TCTGGGTGGA
    TTGTTGACCT CCTCCTACAG GCAGCACCAA GAATCTCTGA ACGCTGAAAG AGAGCGGAGA
    CGTCTGGAGA GAGAAGAGAG ACTTCAACGG ATTGAAAGAG AGGAGAGAAA CCGCTTCAAT
    CGGGATTATG CAGACAAGAG AGAAGAGGTC AGACATGCCC GTGAAGAAAG ATACAAGCAA
    GTGGAGCGTC TGACAGCAGA GGAAAAAGAA AGGGAACGGC CGAGAACACG TGGTAGAACA
    GAAATCACCC TCAGTCTGAG TTCACGCACT TCCAGCAGCT CCTTGTCTCG TTCCGTCACC
    CCTTTGACTG TAGATGCTCA AGAGGAGACA CGCCCTGTCT CACATACAGC ACAAGGAACT
    GTGACTGAGA AAAGTTGTAC GGTGCAGACA GCAGTTGTGC GTTCTTTATC AGAACCTCCC
    TCTGCATCTC CACCAGTGTC CCCATCGCAA CCAGAGGAGC AACAGAGACC TGTCGACCCA
    TCCGACATCA TTCTATCTCT ATCTCCAACA AACTCTGAAC CAGAAAGAAC AGAGAAAGGG
    GAGAAAAAAG AGGATGCTGA AGCAGAGGAG TGTGAGGAAA CAGTGGTGGG TGAAGTGCAA
    GATGAAAAGA AGGAAGAACC AGAAGGAAAC TTTGAGGATG AGACTAATGT GACGGGTGCA
    GAAGACTGTG AGAGGGAGAA GACGTTGGAT AGAGAAGTAG AGGAGAGTGC CATCCTAAGT
    GAAAAGGAGA AGCAGAATGA AGAACTGAAT GAGAAAGATA ACTGCTCTGC CTCCAGCATC
    TCTTCAGCCA GCAGCACTCT GGAGAGAGAA GAAAGAGAGG AGAAACATGT CAGTGACAGC
    AACTCAGGTC AATGGGCAGA CTGTATAAAT ACAGCAGACA ACAACGATCA GTGCAACAAA
    GTTCTTAACA GCAAACGTTT CATGCTGGAC ATGCTGTATG CCCAAAACAA GGAAGGAGAG
    GAAGATGAGA AAGAAAAGGA GAACGAAAAT GAGAAATGTT TAGGTCAGGA CTCTACTGTG
    ACAAGCCTTG CTAGCCGAAT TTCCACTCTG GAATCACATC GGCAGACATG TGAGGACAAC
    TTGAAGAAAC TGGAAGTGGA GCATCTGGAA AACCAGGGCA GCGTGCGGGC TGTAGCTGAG
    AAATTTGGAG ATTTGGTCAA AGGCATGTTA TCACCTCATG ACTTGGAGAA TTGTGGGAAG
    GAAAAGCAGC AAACACAGGA AAAGCCTCCA CCTGCTCCAT CACCAGCACC CCCAAAAAAG
    GAGTCGGACC ACATATGGGA CCAGCTAATG GCAACGCCAC GGGAGCTTCG TATCAAAGAG
    CTGGATTTCA CAGACTTGGG AGAGGAGGAC GATGTGGATA TTTTGGACGT GGGGAACATG
    ATTGGGTCAG GTGACCTCAC TCCACCTCCT CCACCACCGC CCTGCTTGCC CAACCTTCCT
    CCCCCGCCAC CTTTATTTGG ATGCCCTCTG CCTCCCCCAG TCCCTGGCAT GATGATGCCC
    CCTCCACCAC CCTTTTTGGC GTCAAAACCT CCAGCACAAT TGGGATCTCC TCAGCTTAGT
    CGAGGAGAAC CACCGCTCTT CCAAAAGAAG AAGAAGACCA TCAGGCTATT CTGGAATGAG
    GTGAGACCGA TTGACTGGCA GTACACCAAC CACAAGAGAT GCAAGGAGTC ACTCTGGTCC
    AAACTGGATC CCATCAAACT GGACACAGCC AAATTGGAAA ACCTCTTTGA GACGAAATCT
    AAGGAGCTGC CAGTTACAAA GAAAACAGCA GCAGATGGTA AGCGTCAGGA GATCATCATT
    CTGGATTCCA AAAGAAGTAA TGCTATCAAC ATTGGCCTGA CGGTCCTGCC GCCTCCTCGC
    ACCATCAAAA CAGCCATCCT CAACTTTGAT GAGTACGCCT TGAGCAAAGA GGGCATAGAG
    AAAATCCTCA CCATGATTCC TACGGAGGAG GAGACACAGA AGATTCAGGA GGCCCAGCTG
    GCGAACCCCG ACACTCCGCT GGGCAGCGCA GAGCAGTTCC TCCTCATTTT GTCCTCCATC
    AGTGAGCTGT CCGCCCGCCT GCAACTCTGG GCCTTTAAGA TGGACTATGA TGCTTTAGAG
    AAGGAAGTAG CAGAACCACT ACAGGACCTA AAAGAGGGCA TGGACCAATT AGAAAAGAAT
    AAAACGTTAC GCTCTATCCT TTCGACTCTG CTAGCTATTG GCAACTTTCT CAATGGGACC
    AATGCTAAAG GCTTTGAGTT GAGTTATCTA GAGAAAGTTC CTGAAGTGAA GGACACGGTT
    CACAAACAGT CTCTACTGCA TCATGTCTGC TCGATTGTTG TGGAGAAGTT TCCAGAGAGC
    ACAGATCTTT ACTCTGAGAT TGGAGCCATT ACTCGCTCTG CTAAGGTTGA CTTTGATCAG
    CTTCAGGAAA ACCTGTGTCA AATGGAACGG CGCTGCAAAG CATCCTGGGA CCACCTGAAG
    GTCATCTCCA AACACGAGAT TAAACCAGCC CTTAAACAGC GAATGTCCGA CTTCCTCAAA
    GACTGTGCAG GAAGAATTAT CATCCTTAAA ATTGTCCATC GTCGTATCAT AAACAGGTTT
    CACTCATTCC TGCTATTTCT GGGCCACCCA CCATACGCCA TTAGAGAAGT GAGCGTCCAC
    CGCTTCAGCA AGATTCTGAG TGAGTTTGCT CTTGAGTACC GAACCACCAG AGAGCGAGTG
    CTGCAGCAGA AACAGAAACG AGCCAATCAT CGTGAGAGGA ACAAGACCCG CGGCAAAATG
    ATCACAGAGA GTGATGAGGA AGAGGAGGAT GAGAGTGGGA AGTTTGCAGG AAGTCCTATG
    GAGGGTCAGG AGTGTCAACC GCAGGGTTTG GGCTACACAG AGGACGTGAC TGAGCATGAG
    CAGATGACCG CAGTGCTTTG TACCAGCCTG CAGGGTGGCG ATAGTCCATC ATCTGTCCTT
    CCTGGCCTTC GCACACGGAC CCGAGCCACT CGAGGTCATA CAGCACTTTT GTCCACTGCA
    AATGAAGACA CTTCTAATTG CACAAATGAC ACAGCAGATG AGATCATGGA GCGGATTGTG
    AAATCAGCCA CTCAGGGCCC CAGTCAGAGA ACACAGCCTC GTGAGAGGAG ACGTTCCAGA
    GCAAACCGCA AATCCTTGAG AAGAACACTG AAGAACGGTC TTACCCCGGA GGAGGCACAG
    GCTCTGGGTT TGGCCAGCGG CTCTGAGATG CAGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322568.1
    CDS653..5251
    Translation

    Target ORF information:

    RefSeq Version XM_021466893.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio formin homology 2 domain containing 3b (fhod3b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466893.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCTACGT TTGCATGCAG GGTGCAGTTC TTAGATGATA CAGATCCATT TAATAGCACA 
    AATTTTCCGG AGCCGACCAG ACCCCCACAT TTCACATTCA GAGAGGATAT TCCGCTCATC
    AATCAGATCG CCGGAGTTCA CAGACTTCTC AAAGCACCTC ACAAGCTTGA TGACTGTGCT
    TTGCAGTTGT CTCATAATGG CAGTTATCTG GACCTGGAGT CCACCTTGGC TGAGCAGAAA
    GATGAACTTG AAGGCTTTCA GGAGGATGGA GGGAGAGGAA AGAAGCACAG TATTGTACTT
    AGAACACAGC TGTCTGTCAG AGTCCATGCC TGCATAGAAA AGCTGTACAA CTCGACGGGT
    CGTGAGCTAC GAAGAGCACT GTTCTCTCTC AAGCAAATCT TCCAGGATGA TAAGGATCTG
    GTTCATGAGT TTGTGATGGC GGAGGGACTC ACATGTCTTA TTAAAGTGGG AGCTGAAGCT
    GATCAAAACT ACCAAAACTA CATTCTCAGA GCACTTGGAC AGATCATGCT GTATGTAGAT
    GGAATGAATG GGCTTATCGG ACACAATGAA ACCGTACAGT GGCTGTACAC ACTGGTTGGA
    TCAAAGTTCC GGTTGGTTGT GAAAACTGCT ATGAAGCTGC TGCTGGTTTT TGTGGAGTAC
    ACCGAGAGCA ACGCACCTCT CCTCATCCAA GCCGTCAACA GCGTTGACAC CAAACAAGGC
    TCTAAGCCCT GGTCTAATGC CATGGAAATC CTTGACGAGA AAGATGGAAT GGACACTGAG
    CTGCTGGTTT ATGCCATGAC ACTAATCAAC AAGACATTAG CTGGTCTGCC TGACCAGGAC
    TCCTTCTATG ATGTGGTTGA CAGTCTGGAG GAGCAGAACA TGGAGGCTGT TGCCCAAAGA
    CACATTAGTA GAAAGGGCAC AGATCTGGAC TTGGTAGAGC AGTTCAACAT TTATGAGGCA
    ACTCTTCGTC ATGAAGACGG TGATGATGAA GCTCAGTCTC CTCCATCTGG ACATAAATGT
    CGGCGTCGGG CTAGTGTTGG AGCAACACCT GAGAGACGGG GTTTGGAACG ACGGCGTAGC
    CGAAGACATT CACTCCAGAA CGGAAAAGGC CACACTTCTG CTCCAACTTC CCCCTGTCAC
    AGCCATACAC CCAACATCCT GTCCTTTAAC AGCCAGAGGA TAGAAGACAT CAGTGAAAGT
    TTGTCAGATG TTAGTTACCC AAACACACCA TGCAGTTCTT GTGGCAGCAA TGGCAATGCA
    AGCACATATC CCGGCAGGTC ATCTGGGGTG TCCAGTGCCC TCTCCACACC CTACAACCGC
    TTCGGTCCTT CAGGAGGTCC ATCTGCAGAC AGTAAAGCCG ACAGGCCAAC TCTGGGTGGA
    TTGTTGACCT CCTCCTACAG GCAGCACCAA GAATCTCTGA ACGCTGAAAG AGAGCGGAGA
    CGTCTGGAGA GAGAAGAGAG ACTTCAACGG ATTGAAAGAG AGGAGAGAAA CCGCTTCAAT
    CGGGATTATG CAGACAAGAG AGAAGAGGTC AGACATGCCC GTGAAGAAAG ATACAAGCAA
    GTGGAGCGTC TGACAGCAGA GGAAAAAGAA AGGGAACGGC CGAGAACACG TGGTAGAACA
    GAAATCACCC TCAGTCTGAG TTCACGCACT TCCAGCAGCT CCTTGTCTCG TTCCGTCACC
    CCTTTGACTG TAGATGCTCA AGAGGAGACA CGCCCTGTCT CACATACAGC ACAAGGAACT
    GTGACTGAGA AAAGTTGTAC GGTGCAGACA GCAGTTGTGC GTTCTTTATC AGAACCTCCC
    TCTGCATCTC CACCAGTGTC CCCATCGCAA CCAGAGGAGC AACAGAGACC TGTCGACCCA
    TCCGACATCA TTCTATCTCT ATCTCCAACA AACTCTGAAC CAGAAAGAAC AGAGAAAGGG
    GAGAAAAAAG AGGATGCTGA AGCAGAGGAG TGTGAGGAAA CAGTGGTGGG TGAAGTGCAA
    GATGAAAAGA AGGAAGAACC AGAAGGAAAC TTTGAGGATG AGACTAATGT GACGGGTGCA
    GAAGACTGTG AGAGGGAGAA GACGTTGGAT AGAGAAGTAG AGGAGAGTGC CATCCTAAGT
    GAAAAGGAGA AGCAGAATGA AGAACTGAAT GAGAAAGATA ACTGCTCTGC CTCCAGCATC
    TCTTCAGCCA GCAGCACTCT GGAGAGAGAA GAAAGAGAGG AGAAACATGT CAGTGACAGC
    AACTCAGGTC AATGGGCAGA CTGTATAAAT ACAGCAGACA ACAACGATCA GTGCAACAAA
    GTTCTTAACA GCAAACGTTT CATGCTGGAC ATGCTGTATG CCCAAAACAA GGAAGGAGAG
    GAAGATGAGA AAGAAAAGGA GAACGAAAAT GAGAAATGTT TAGGTCAGGA CTCTACTGTG
    ACAAGCCTTG CTAGCCGAAT TTCCACTCTG GAATCACATC GGCAGACATG TGAGGACAAC
    TTGAAGAAAC TGGAAGTGGA GCATCTGGAA AACCAGGGCA GCGTGCGGGC TGTAGCTGAG
    AAATTTGGAG ATTTGGTCAA AGGCATGTTA TCACCTCATG ACTTGGAGAA TTGTGGGAAG
    GAAAAGCAGC AAACACAGGA AAAGCCTCCA CCTGCTCCAT CACCAGCACC CCCAAAAAAG
    GAGTCGGACC ACATATGGGA CCAGCTAATG GCAACGCCAC GGGAGCTTCG TATCAAAGAG
    CTGGATTTCA CAGACTTGGG AGAGGAGGAC GATGTGGATA TTTTGGACGT GGGGAACATG
    ATTGGGTCAG GTGACCTCAC TCCACCTCCT CCACCACCGC CCTGCTTGCC CAACCTTCCT
    CCCCCGCCAC CTTTATTTGG ATGCCCTCTG CCTCCCCCAG TCCCTGGCAT GATGATGCCC
    CCTCCACCAC CCTTTTTGGC GTCAAAACCT CCAGCACAAT TGGGATCTCC TCAGCTTAGT
    CGAGGAGAAC CACCGCTCTT CCAAAAGAAG AAGAAGACCA TCAGGCTATT CTGGAATGAG
    GTGAGACCGA TTGACTGGCA GTACACCAAC CACAAGAGAT GCAAGGAGTC ACTCTGGTCC
    AAACTGGATC CCATCAAACT GGACACAGCC AAATTGGAAA ACCTCTTTGA GACGAAATCT
    AAGGAGCTGC CAGTTACAAA GAAAACAGCA GCAGATGGTA AGCGTCAGGA GATCATCATT
    CTGGATTCCA AAAGAAGTAA TGCTATCAAC ATTGGCCTGA CGGTCCTGCC GCCTCCTCGC
    ACCATCAAAA CAGCCATCCT CAACTTTGAT GAGTACGCCT TGAGCAAAGA GGGCATAGAG
    AAAATCCTCA CCATGATTCC TACGGAGGAG GAGACACAGA AGATTCAGGA GGCCCAGCTG
    GCGAACCCCG ACACTCCGCT GGGCAGCGCA GAGCAGTTCC TCCTCATTTT GTCCTCCATC
    AGTGAGCTGT CCGCCCGCCT GCAACTCTGG GCCTTTAAGA TGGACTATGA TGCTTTAGAG
    AAGGAAGTAG CAGAACCACT ACAGGACCTA AAAGAGGGCA TGGACCAATT AGAAAAGAAT
    AAAACGTTAC GCTCTATCCT TTCGACTCTG CTAGCTATTG GCAACTTTCT CAATGGGACC
    AATGCTAAAG GCTTTGAGTT GAGTTATCTA GAGAAAGTTC CTGAAGTGAA GGACACGGTT
    CACAAACAGT CTCTACTGCA TCATGTCTGC TCGATTGTTG TGGAGAAGTT TCCAGAGAGC
    ACAGATCTTT ACTCTGAGAT TGGAGCCATT ACTCGCTCTG CTAAGGTTGA CTTTGATCAG
    CTTCAGGAAA ACCTGTGTCA AATGGAACGG CGCTGCAAAG CATCCTGGGA CCACCTGAAG
    GTCATCTCCA AACACGAGAT TAAACCAGCC CTTAAACAGC GAATGTCCGA CTTCCTCAAA
    GACTGTGCAG GAAGAATTAT CATCCTTAAA ATTGTCCATC GTCGTATCAT AAACAGGTTT
    CACTCATTCC TGCTATTTCT GGGCCACCCA CCATACGCCA TTAGAGAAGT GAGCGTCCAC
    CGCTTCAGCA AGATTCTGAG TGAGTTTGCT CTTGAGTACC GAACCACCAG AGAGCGAGTG
    CTGCAGCAGA AACAGAAACG AGCCAATCAT CGTGAGAGGA ACAAGACCCG CGGCAAAATG
    ATCACAGAGA GTGATGAGGA AGAGGAGGAT GAGAGTGGGA AGTTTGCAGG AAGTCCTATG
    GAGGGTCAGG AGTGTCAACC GCAGGGTTTG GGCTACACAG AGGACGTGAC TGAGCATGAG
    CAGATGACCG CAGTGCTTTG TACCAGCCTG CAGGGTGGCG ATAGTCCATC ATCTGTCCTT
    CCTGGCCTTC GCACACGGAC CCGAGCCACT CGAGGTCATA CAGCACTTTT GTCCACTGCA
    AATGAAGACA CTTCTAATTG CACAAATGAC ACAGCAGATG AGATCATGGA GCGGATTGTG
    AAATCAGCCA CTCAGGGCCC CAGTCAGAGA ACACAGCCTC GTGAGAGGAG ACGTTCCAGA
    GCAAACCGCA AATCCTTGAG AAGAACACTG AAGAACGGTC TTACCCCGGA GGAGGCACAG
    GCTCTGGGTT TGGCCAGCGG CTCTGAGATG CAGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149379
    Clone ID Related Accession (Same CDS sequence) XM_021466894.1
    Accession Version XM_021466894.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4551bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product FH1/FH2 domain-containing protein 3 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTACGT TTGCATGCAG GGTGCAGTTC TTAGATGATA CAGATCCATT TAATAGCACA 
    AATTTTCCGG AGCCGACCAG ACCCCCACAT TTCACATTCA GAGAGGATAT TCCGCTCATC
    AATCAGATCG CCGGAGTTCA CAGACTTCTC AAAGCACCTC ACAAGCTTGA TGACTGTGCT
    TTGCAGTTGT CTCATAATGG CAGTTATCTG GACCTGGAGT CCACCTTGGC TGAGCAGAAA
    GATGAACTTG AAGGCTTTCA GGAGGATGGA GGGAGAGGAA AGAAGCACAG TATTGTACTT
    AGAACACAGC TGTCTGTCAG AGTCCATGCC TGCATAGAAA AGCTGTACAA CTCGACGGGT
    CGTGAGCTAC GAAGAGCACT GTTCTCTCTC AAGCAAATCT TCCAGGATGA TAAGGATCTG
    GTTCATGAGT TTGTGATGGC GGAGGGACTC ACATGTCTTA TTAAAGTGGG AGCTGAAGCT
    GATCAAAACT ACCAAAACTA CATTCTCAGA GCACTTGGAC AGATCATGCT GTATGTAGAT
    GGAATGAATG GGCTTATCGG ACACAATGAA ACCGTACAGT GGCTGTACAC ACTGGTTGGA
    TCAAAGTTCC GGTTGGTTGT GAAAACTGCT ATGAAGCTGC TGCTGGTTTT TGTGGAGTAC
    ACCGAGAGCA ACGCACCTCT CCTCATCCAA GCCGTCAACA GCGTTGACAC CAAACAAGGC
    TCTAAGCCCT GGTCTAATGC CATGGAAATC CTTGACGAGA AAGATGGAAT GGACACTGAG
    CTGCTGGTTT ATGCCATGAC ACTAATCAAC AAGACATTAG CTGGTCTGCC TGACCAGGAC
    TCCTTCTATG ATGTGGTTGA CAGTCTGGAG GAGCAGAACA TGGAGGCTGT TGCCCAAAGA
    CACATTAGTA GAAAGGGCAC AGATCTGGAC TTGGTAGAGC AGTTCAACAT TTATGAGGCA
    ACTCTTCGTC ATGAAGACGG TGATGATGAA GCTCAGTCTC CTCCATCTGG ACATAAATGT
    CGGCGTCGGG CTAGTGTTGG AGCAACACCT GAGAGACGGG GTTTGGAACG ACGGCGTAGC
    CGAAGACATT CACTCCAGAA CGGAAAAGGC CACACTTCTG CTCCAACTTC CCCCTGTCAC
    AGCCATACAC CCAACATCCT GTCCTTTAAC AGCCAGAGGA TAGAAGACAT CAGTGAAAGC
    AATGGCAATG CAAGCACATA TCCCGGCAGG TCATCTGGGG TGTCCAGTGC CCTCTCCACA
    CCCTACAACC GCTTCGGTCC TTCAGGAGGT CCATCTGCAG ACAGTAAAGC CGACAGGCCA
    ACTCTGGGTG GATTGTTGAC CTCCTCCTAC AGGCAGCACC AAGAATCTCT GAACGCTGAA
    AGAGAGCGGA GACGTCTGGA GAGAGAAGAG AGACTTCAAC GGATTGAAAG AGAGGAGAGA
    AACCGCTTCA ATCGGGATTA TGCAGACAAG AGAGAAGAGG TCAGACATGC CCGTGAAGAA
    AGATACAAGC AAGTGGAGCG TCTGACAGCA GAGGAAAAAG AAAGGGAACG GCCGAGAACA
    CGTGGTAGAA CAGAAATCAC CCTCAGTCTG AGTTCACGCA CTTCCAGCAG CTCCTTGTCT
    CGTTCCGTCA CCCCTTTGAC TGTAGATGCT CAAGAGGAGA CACGCCCTGT CTCACATACA
    GCACAAGGAA CTGTGACTGA GAAAAGTTGT ACGGTGCAGA CAGCAGTTGT GCGTTCTTTA
    TCAGAACCTC CCTCTGCATC TCCACCAGTG TCCCCATCGC AACCAGAGGA GCAACAGAGA
    CCTGTCGACC CATCCGACAT CATTCTATCT CTATCTCCAA CAAACTCTGA ACCAGAAAGA
    ACAGAGAAAG GGGAGAAAAA AGAGGATGCT GAAGCAGAGG AGTGTGAGGA AACAGTGGTG
    GGTGAAGTGC AAGATGAAAA GAAGGAAGAA CCAGAAGGAA ACTTTGAGGA TGAGACTAAT
    GTGACGGGTG CAGAAGACTG TGAGAGGGAG AAGACGTTGG ATAGAGAAGT AGAGGAGAGT
    GCCATCCTAA GTGAAAAGGA GAAGCAGAAT GAAGAACTGA ATGAGAAAGA TAACTGCTCT
    GCCTCCAGCA TCTCTTCAGC CAGCAGCACT CTGGAGAGAG AAGAAAGAGA GGAGAAACAT
    GTCAGTGACA GCAACTCAGG TCAATGGGCA GACTGTATAA ATACAGCAGA CAACAACGAT
    CAGTGCAACA AAGTTCTTAA CAGCAAACGT TTCATGCTGG ACATGCTGTA TGCCCAAAAC
    AAGGAAGGAG AGGAAGATGA GAAAGAAAAG GAGAACGAAA ATGAGAAATG TTTAGGTCAG
    GACTCTACTG TGACAAGCCT TGCTAGCCGA ATTTCCACTC TGGAATCACA TCGGCAGACA
    TGTGAGGACA ACTTGAAGAA ACTGGAAGTG GAGCATCTGG AAAACCAGGG CAGCGTGCGG
    GCTGTAGCTG AGAAATTTGG AGATTTGGTC AAAGGCATGT TATCACCTCA TGACTTGGAG
    AATTGTGGGA AGGAAAAGCA GCAAACACAG GAAAAGCCTC CACCTGCTCC ATCACCAGCA
    CCCCCAAAAA AGGAGTCGGA CCACATATGG GACCAGCTAA TGGCAACGCC ACGGGAGCTT
    CGTATCAAAG AGCTGGATTT CACAGACTTG GGAGAGGAGG ACGATGTGGA TATTTTGGAC
    GTGGGGAACA TGATTGGGTC AGGTGACCTC ACTCCACCTC CTCCACCACC GCCCTGCTTG
    CCCAACCTTC CTCCCCCGCC ACCTTTATTT GGATGCCCTC TGCCTCCCCC AGTCCCTGGC
    ATGATGATGC CCCCTCCACC ACCCTTTTTG GCGTCAAAAC CTCCAGCACA ATTGGGATCT
    CCTCAGCTTA GTCGAGGAGA ACCACCGCTC TTCCAAAAGA AGAAGAAGAC CATCAGGCTA
    TTCTGGAATG AGGTGAGACC GATTGACTGG CAGTACACCA ACCACAAGAG ATGCAAGGAG
    TCACTCTGGT CCAAACTGGA TCCCATCAAA CTGGACACAG CCAAATTGGA AAACCTCTTT
    GAGACGAAAT CTAAGGAGCT GCCAGTTACA AAGAAAACAG CAGCAGATGG TAAGCGTCAG
    GAGATCATCA TTCTGGATTC CAAAAGAAGT AATGCTATCA ACATTGGCCT GACGGTCCTG
    CCGCCTCCTC GCACCATCAA AACAGCCATC CTCAACTTTG ATGAGTACGC CTTGAGCAAA
    GAGGGCATAG AGAAAATCCT CACCATGATT CCTACGGAGG AGGAGACACA GAAGATTCAG
    GAGGCCCAGC TGGCGAACCC CGACACTCCG CTGGGCAGCG CAGAGCAGTT CCTCCTCATT
    TTGTCCTCCA TCAGTGAGCT GTCCGCCCGC CTGCAACTCT GGGCCTTTAA GATGGACTAT
    GATGCTTTAG AGAAGGAAGT AGCAGAACCA CTACAGGACC TAAAAGAGGG CATGGACCAA
    TTAGAAAAGA ATAAAACGTT ACGCTCTATC CTTTCGACTC TGCTAGCTAT TGGCAACTTT
    CTCAATGGGA CCAATGCTAA AGGCTTTGAG TTGAGTTATC TAGAGAAAGT TCCTGAAGTG
    AAGGACACGG TTCACAAACA GTCTCTACTG CATCATGTCT GCTCGATTGT TGTGGAGAAG
    TTTCCAGAGA GCACAGATCT TTACTCTGAG ATTGGAGCCA TTACTCGCTC TGCTAAGGTT
    GACTTTGATC AGCTTCAGGA AAACCTGTGT CAAATGGAAC GGCGCTGCAA AGCATCCTGG
    GACCACCTGA AGGTCATCTC CAAACACGAG ATTAAACCAG CCCTTAAACA GCGAATGTCC
    GACTTCCTCA AAGACTGTGC AGGAAGAATT ATCATCCTTA AAATTGTCCA TCGTCGTATC
    ATAAACAGGT TTCACTCATT CCTGCTATTT CTGGGCCACC CACCATACGC CATTAGAGAA
    GTGAGCGTCC ACCGCTTCAG CAAGATTCTG AGTGAGTTTG CTCTTGAGTA CCGAACCACC
    AGAGAGCGAG TGCTGCAGCA GAAACAGAAA CGAGCCAATC ATCGTGAGAG GAACAAGACC
    CGCGGCAAAA TGATCACAGA GAGTGATGAG GAAGAGGAGG ATGAGAGTGG GAAGTTTGCA
    GGAAGTCCTA TGGAGGGTCA GGAGTGTCAA CCGCAGGGTT TGGGCTACAC AGAGGACGTG
    ACTGAGCATG AGCAGATGAC CGCAGTGCTT TGTACCAGCC TGCAGGGTGG CGATAGTCCA
    TCATCTGTCC TTCCTGGCCT TCGCACACGG ACCCGAGCCA CTCGAGGTCA TACAGCACTT
    TTGTCCACTG CAAATGAAGA CACTTCTAAT TGCACAAATG ACACAGCAGA TGAGATCATG
    GAGCGGATTG TGAAATCAGC CACTCAGGGC CCCAGTCAGA GAACACAGCC TCGTGAGAGG
    AGACGTTCCA GAGCAAACCG CAAATCCTTG AGAAGAACAC TGAAGAACGG TCTTACCCCG
    GAGGAGGCAC AGGCTCTGGG TTTGGCCAGC GGCTCTGAGA TGCAGGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322569.1
    CDS653..5203
    Translation

    Target ORF information:

    RefSeq Version XM_021466894.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio formin homology 2 domain containing 3b (fhod3b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466894.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTACGT TTGCATGCAG GGTGCAGTTC TTAGATGATA CAGATCCATT TAATAGCACA 
    AATTTTCCGG AGCCGACCAG ACCCCCACAT TTCACATTCA GAGAGGATAT TCCGCTCATC
    AATCAGATCG CCGGAGTTCA CAGACTTCTC AAAGCACCTC ACAAGCTTGA TGACTGTGCT
    TTGCAGTTGT CTCATAATGG CAGTTATCTG GACCTGGAGT CCACCTTGGC TGAGCAGAAA
    GATGAACTTG AAGGCTTTCA GGAGGATGGA GGGAGAGGAA AGAAGCACAG TATTGTACTT
    AGAACACAGC TGTCTGTCAG AGTCCATGCC TGCATAGAAA AGCTGTACAA CTCGACGGGT
    CGTGAGCTAC GAAGAGCACT GTTCTCTCTC AAGCAAATCT TCCAGGATGA TAAGGATCTG
    GTTCATGAGT TTGTGATGGC GGAGGGACTC ACATGTCTTA TTAAAGTGGG AGCTGAAGCT
    GATCAAAACT ACCAAAACTA CATTCTCAGA GCACTTGGAC AGATCATGCT GTATGTAGAT
    GGAATGAATG GGCTTATCGG ACACAATGAA ACCGTACAGT GGCTGTACAC ACTGGTTGGA
    TCAAAGTTCC GGTTGGTTGT GAAAACTGCT ATGAAGCTGC TGCTGGTTTT TGTGGAGTAC
    ACCGAGAGCA ACGCACCTCT CCTCATCCAA GCCGTCAACA GCGTTGACAC CAAACAAGGC
    TCTAAGCCCT GGTCTAATGC CATGGAAATC CTTGACGAGA AAGATGGAAT GGACACTGAG
    CTGCTGGTTT ATGCCATGAC ACTAATCAAC AAGACATTAG CTGGTCTGCC TGACCAGGAC
    TCCTTCTATG ATGTGGTTGA CAGTCTGGAG GAGCAGAACA TGGAGGCTGT TGCCCAAAGA
    CACATTAGTA GAAAGGGCAC AGATCTGGAC TTGGTAGAGC AGTTCAACAT TTATGAGGCA
    ACTCTTCGTC ATGAAGACGG TGATGATGAA GCTCAGTCTC CTCCATCTGG ACATAAATGT
    CGGCGTCGGG CTAGTGTTGG AGCAACACCT GAGAGACGGG GTTTGGAACG ACGGCGTAGC
    CGAAGACATT CACTCCAGAA CGGAAAAGGC CACACTTCTG CTCCAACTTC CCCCTGTCAC
    AGCCATACAC CCAACATCCT GTCCTTTAAC AGCCAGAGGA TAGAAGACAT CAGTGAAAGC
    AATGGCAATG CAAGCACATA TCCCGGCAGG TCATCTGGGG TGTCCAGTGC CCTCTCCACA
    CCCTACAACC GCTTCGGTCC TTCAGGAGGT CCATCTGCAG ACAGTAAAGC CGACAGGCCA
    ACTCTGGGTG GATTGTTGAC CTCCTCCTAC AGGCAGCACC AAGAATCTCT GAACGCTGAA
    AGAGAGCGGA GACGTCTGGA GAGAGAAGAG AGACTTCAAC GGATTGAAAG AGAGGAGAGA
    AACCGCTTCA ATCGGGATTA TGCAGACAAG AGAGAAGAGG TCAGACATGC CCGTGAAGAA
    AGATACAAGC AAGTGGAGCG TCTGACAGCA GAGGAAAAAG AAAGGGAACG GCCGAGAACA
    CGTGGTAGAA CAGAAATCAC CCTCAGTCTG AGTTCACGCA CTTCCAGCAG CTCCTTGTCT
    CGTTCCGTCA CCCCTTTGAC TGTAGATGCT CAAGAGGAGA CACGCCCTGT CTCACATACA
    GCACAAGGAA CTGTGACTGA GAAAAGTTGT ACGGTGCAGA CAGCAGTTGT GCGTTCTTTA
    TCAGAACCTC CCTCTGCATC TCCACCAGTG TCCCCATCGC AACCAGAGGA GCAACAGAGA
    CCTGTCGACC CATCCGACAT CATTCTATCT CTATCTCCAA CAAACTCTGA ACCAGAAAGA
    ACAGAGAAAG GGGAGAAAAA AGAGGATGCT GAAGCAGAGG AGTGTGAGGA AACAGTGGTG
    GGTGAAGTGC AAGATGAAAA GAAGGAAGAA CCAGAAGGAA ACTTTGAGGA TGAGACTAAT
    GTGACGGGTG CAGAAGACTG TGAGAGGGAG AAGACGTTGG ATAGAGAAGT AGAGGAGAGT
    GCCATCCTAA GTGAAAAGGA GAAGCAGAAT GAAGAACTGA ATGAGAAAGA TAACTGCTCT
    GCCTCCAGCA TCTCTTCAGC CAGCAGCACT CTGGAGAGAG AAGAAAGAGA GGAGAAACAT
    GTCAGTGACA GCAACTCAGG TCAATGGGCA GACTGTATAA ATACAGCAGA CAACAACGAT
    CAGTGCAACA AAGTTCTTAA CAGCAAACGT TTCATGCTGG ACATGCTGTA TGCCCAAAAC
    AAGGAAGGAG AGGAAGATGA GAAAGAAAAG GAGAACGAAA ATGAGAAATG TTTAGGTCAG
    GACTCTACTG TGACAAGCCT TGCTAGCCGA ATTTCCACTC TGGAATCACA TCGGCAGACA
    TGTGAGGACA ACTTGAAGAA ACTGGAAGTG GAGCATCTGG AAAACCAGGG CAGCGTGCGG
    GCTGTAGCTG AGAAATTTGG AGATTTGGTC AAAGGCATGT TATCACCTCA TGACTTGGAG
    AATTGTGGGA AGGAAAAGCA GCAAACACAG GAAAAGCCTC CACCTGCTCC ATCACCAGCA
    CCCCCAAAAA AGGAGTCGGA CCACATATGG GACCAGCTAA TGGCAACGCC ACGGGAGCTT
    CGTATCAAAG AGCTGGATTT CACAGACTTG GGAGAGGAGG ACGATGTGGA TATTTTGGAC
    GTGGGGAACA TGATTGGGTC AGGTGACCTC ACTCCACCTC CTCCACCACC GCCCTGCTTG
    CCCAACCTTC CTCCCCCGCC ACCTTTATTT GGATGCCCTC TGCCTCCCCC AGTCCCTGGC
    ATGATGATGC CCCCTCCACC ACCCTTTTTG GCGTCAAAAC CTCCAGCACA ATTGGGATCT
    CCTCAGCTTA GTCGAGGAGA ACCACCGCTC TTCCAAAAGA AGAAGAAGAC CATCAGGCTA
    TTCTGGAATG AGGTGAGACC GATTGACTGG CAGTACACCA ACCACAAGAG ATGCAAGGAG
    TCACTCTGGT CCAAACTGGA TCCCATCAAA CTGGACACAG CCAAATTGGA AAACCTCTTT
    GAGACGAAAT CTAAGGAGCT GCCAGTTACA AAGAAAACAG CAGCAGATGG TAAGCGTCAG
    GAGATCATCA TTCTGGATTC CAAAAGAAGT AATGCTATCA ACATTGGCCT GACGGTCCTG
    CCGCCTCCTC GCACCATCAA AACAGCCATC CTCAACTTTG ATGAGTACGC CTTGAGCAAA
    GAGGGCATAG AGAAAATCCT CACCATGATT CCTACGGAGG AGGAGACACA GAAGATTCAG
    GAGGCCCAGC TGGCGAACCC CGACACTCCG CTGGGCAGCG CAGAGCAGTT CCTCCTCATT
    TTGTCCTCCA TCAGTGAGCT GTCCGCCCGC CTGCAACTCT GGGCCTTTAA GATGGACTAT
    GATGCTTTAG AGAAGGAAGT AGCAGAACCA CTACAGGACC TAAAAGAGGG CATGGACCAA
    TTAGAAAAGA ATAAAACGTT ACGCTCTATC CTTTCGACTC TGCTAGCTAT TGGCAACTTT
    CTCAATGGGA CCAATGCTAA AGGCTTTGAG TTGAGTTATC TAGAGAAAGT TCCTGAAGTG
    AAGGACACGG TTCACAAACA GTCTCTACTG CATCATGTCT GCTCGATTGT TGTGGAGAAG
    TTTCCAGAGA GCACAGATCT TTACTCTGAG ATTGGAGCCA TTACTCGCTC TGCTAAGGTT
    GACTTTGATC AGCTTCAGGA AAACCTGTGT CAAATGGAAC GGCGCTGCAA AGCATCCTGG
    GACCACCTGA AGGTCATCTC CAAACACGAG ATTAAACCAG CCCTTAAACA GCGAATGTCC
    GACTTCCTCA AAGACTGTGC AGGAAGAATT ATCATCCTTA AAATTGTCCA TCGTCGTATC
    ATAAACAGGT TTCACTCATT CCTGCTATTT CTGGGCCACC CACCATACGC CATTAGAGAA
    GTGAGCGTCC ACCGCTTCAG CAAGATTCTG AGTGAGTTTG CTCTTGAGTA CCGAACCACC
    AGAGAGCGAG TGCTGCAGCA GAAACAGAAA CGAGCCAATC ATCGTGAGAG GAACAAGACC
    CGCGGCAAAA TGATCACAGA GAGTGATGAG GAAGAGGAGG ATGAGAGTGG GAAGTTTGCA
    GGAAGTCCTA TGGAGGGTCA GGAGTGTCAA CCGCAGGGTT TGGGCTACAC AGAGGACGTG
    ACTGAGCATG AGCAGATGAC CGCAGTGCTT TGTACCAGCC TGCAGGGTGG CGATAGTCCA
    TCATCTGTCC TTCCTGGCCT TCGCACACGG ACCCGAGCCA CTCGAGGTCA TACAGCACTT
    TTGTCCACTG CAAATGAAGA CACTTCTAAT TGCACAAATG ACACAGCAGA TGAGATCATG
    GAGCGGATTG TGAAATCAGC CACTCAGGGC CCCAGTCAGA GAACACAGCC TCGTGAGAGG
    AGACGTTCCA GAGCAAACCG CAAATCCTTG AGAAGAACAC TGAAGAACGG TCTTACCCCG
    GAGGAGGCAC AGGCTCTGGG TTTGGCCAGC GGCTCTGAGA TGCAGGTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa21317
    Clone ID Related Accession (Same CDS sequence) XM_005157949.3
    Accession Version XM_005157949.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4650bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product FH1/FH2 domain-containing protein 3 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157949.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTACGT TTGCATGCAG GGTGCAGTTC TTAGATGATA CAGATCCATT TAATAGCACA 
    AATTTTCCGG AGCCGACCAG ACCCCCACAT TTCACATTCA GAGAGGATAT TCCGCTCATC
    AATCAGATCG CCGGAGTTCA CAGACTTCTC AAAGCACCTC ACAAGCTTGA TGACTGTGCT
    TTGCAGTTGT CTCATAATGG CAGTTATCTG GACCTGGAGT CCACCTTGGC TGAGCAGAAA
    GATGAACTTG AAGGCTTTCA GGAGGATGGA GGGAGAGGAA AGAAGCACAG TATTGTACTT
    AGAACACAGC TGTCTGTCAG AGTCCATGCC TGCATAGAAA AGCTGTACAA CTCGACGGGT
    CGTGAGCTAC GAAGAGCACT GTTCTCTCTC AAGCAAATCT TCCAGGATGA TAAGGATCTG
    GTTCATGAGT TTGTGATGGC GGAGGGACTC ACATGTCTTA TTAAAGTGGG AGCTGAAGCT
    GATCAAAACT ACCAAAACTA CATTCTCAGA GCACTTGGAC AGATCATGCT GTATGTAGAT
    GGAATGAATG GGCTTATCGG ACACAATGAA ACCGTACAGT GGCTGTACAC ACTGGTTGGA
    TCAAAGTTCC GGTTGGTTGT GAAAACTGCT ATGAAGCTGC TGCTGGTTTT TGTGGAGTAC
    ACCGAGAGCA ACGCACCTCT CCTCATCCAA GCCGTCAACA GCGTTGACAC CAAACAAGGC
    TCTAAGCCCT GGTCTAATGC CATGGAAATC CTTGACGAGA AAGATGGAAT GGACACTGAG
    CTGCTGGTTT ATGCCATGAC ACTAATCAAC AAGACATTAG CTGGTCTGCC TGACCAGGAC
    TCCTTCTATG ATGTGGTTGA CAGTCTGGAG GAGCAGAACA TGGAGGCTGT TGCCCAAAGA
    CACATTAGTA GAAAGGGCAC AGATCTGGAC TTGGTAGAGC AGTTCAACAT TTATGAGGCA
    ACTCTTCGTC ATGAAGACGG TGATGATGAA GCTCAGTCTC CTCCATCTGG ACATAAATGT
    CGGCGTCGGG CTAGTGTTGG AGCAACACCT GAGAGACGGG GTTTGGAACG ACGGCGTAGC
    CGAAGACATT CACTCCAGAA CGGAAAAGGC CACACTTCTG CTCCAACTTC CCCCTGTCAC
    AGCCATACAC CCAACATCCT GTCCTTTAAC AGCCAGAGGA TAGAAGACAT CAGTGAAAGA
    GAGGATGAGG AAGAGGAGGA GGAAAAGGAT GAAGAGTTTC AGAAAGCAGC ACTTTCTAGA
    CAGGCATCTG GAAGTTTGTC AGATGTTAGT TACCCAAACA CACCATGCAG TTCTTGTGGC
    AGCAATGGCA ATGCAAGCAC ATATCCCGGC AGGTCATCTG GGGTGTCCAG TGCCCTCTCC
    ACACCCTACA ACCGCTTCGG TCCTTCAGGA GGTCCATCTG CAGACAGTAA AGCCGACAGG
    CCAACTCTGG GTGGATTGTT GACCTCCTCC TACAGGCAGC ACCAAGAATC TCTGAACGCT
    GAAAGAGAGC GGAGACGTCT GGAGAGAGAA GAGAGACTTC AACGGATTGA AAGAGAGGAG
    AGAAACCGCT TCAATCGGGA TTATGCAGAC AAGAGAGAAG AGGTCAGACA TGCCCGTGAA
    GAAAGATACA AGCAAGTGGA GCGTCTGACA GCAGAGGAAA AAGAAAGGGA ACGGCCGAGA
    ACACGTGGTA GAACAGAAAT CACCCTCAGT CTGAGTTCAC GCACTTCCAG CAGCTCCTTG
    TCTCGTTCCG TCACCCCTTT GACTGTAGAT GCTCAAGAGG AGACACGCCC TGTCTCACAT
    ACAGCACAAG GAACTGTGAC TGAGAAAAGT TGTACGGTGC AGACAGCAGT TGTGCGTTCT
    TTATCAGAAC CTCCCTCTGC ATCTCCACCA GTGTCCCCAT CGCAACCAGA GGAGCAACAG
    AGACCTGTCG ACCCATCCGA CATCATTCTA TCTCTATCTC CAACAAACTC TGAACCAGAA
    AGAACAGAGA AAGGGGAGAA AAAAGAGGAT GCTGAAGCAG AGGAGTGTGA GGAAACAGTG
    GTGGGTGAAG TGCAAGATGA AAAGAAGGAA GAACCAGAAG GAAACTTTGA GGATGAGACT
    AATGTGACGG GTGCAGAAGA CTGTGAGAGG GAGAAGACGT TGGATAGAGA AGTAGAGGAG
    AGTGCCATCC TAAGTGAAAA GGAGAAGCAG AATGAAGAAC TGAATGAGAA AGATAACTGC
    TCTGCCTCCA GCATCTCTTC AGCCAGCAGC ACTCTGGAGA GAGAAGAAAG AGAGGAGAAA
    CATGTCAGTG ACAGCAACTC AGGTCAATGG GCAGACTGTA TAAATACAGC AGACAACAAC
    GATCAGTGCA ACAAAGTTCT TAACAGCAAA CGTTTCATGC TGGACATGCT GTATGCCCAA
    AACAAGGAAG GAGAGGAAGA TGAGAAAGAA AAGGAGAACG AAAATGAGAA ATGTTTAGGT
    CAGGACTCTA CTGTGACAAG CCTTGCTAGC CGAATTTCCA CTCTGGAATC ACATCGGCAG
    ACATGTGAGG ACAACTTGAA GAAACTGGAA GTGGAGCATC TGGAAAACCA GGGCAGCGTG
    CGGGCTGTAG CTGAGAAATT TGGAGATTTG GTCAAAGGCA TGTTATCACC TCATGACTTG
    GAGAATTGTG GGAAGGAAAA GCAGCAAACA CAGGAAAAGC CTCCACCTGC TCCATCACCA
    GCACCCCCAA AAAAGGAGTC GGACCACATA TGGGACCAGC TAATGGCAAC GCCACGGGAG
    CTTCGTATCA AAGAGCTGGA TTTCACAGAC TTGGGAGAGG AGGACGATGT GGATATTTTG
    GACGTGGGGA ACATGATTGG GTCAGGTGAC CTCACTCCAC CTCCTCCACC ACCGCCCTGC
    TTGCCCAACC TTCCTCCCCC GCCACCTTTA TTTGGATGCC CTCTGCCTCC CCCAGTCCCT
    GGCATGATGA TGCCCCCTCC ACCACCCTTT TTGGCGTCAA AACCTCCAGC ACAATTGGGA
    TCTCCTCAGC TTAGTCGAGG AGAACCACCG CTCTTCCAAA AGAAGAAGAA GACCATCAGG
    CTATTCTGGA ATGAGGTGAG ACCGATTGAC TGGCAGTACA CCAACCACAA GAGATGCAAG
    GAGTCACTCT GGTCCAAACT GGATCCCATC AAACTGGACA CAGCCAAATT GGAAAACCTC
    TTTGAGACGA AATCTAAGGA GCTGCCAGTT ACAAAGAAAA CAGCAGCAGA TGGTAAGCGT
    CAGGAGATCA TCATTCTGGA TTCCAAAAGA AGTAATGCTA TCAACATTGG CCTGACGGTC
    CTGCCGCCTC CTCGCACCAT CAAAACAGCC ATCCTCAACT TTGATGAGTA CGCCTTGAGC
    AAAGAGGGCA TAGAGAAAAT CCTCACCATG ATTCCTACGG AGGAGGAGAC ACAGAAGATT
    CAGGAGGCCC AGCTGGCGAA CCCCGACACT CCGCTGGGCA GCGCAGAGCA GTTCCTCCTC
    ATTTTGTCCT CCATCAGTGA GCTGTCCGCC CGCCTGCAAC TCTGGGCCTT TAAGATGGAC
    TATGATGCTT TAGAGAAGGA AGTAGCAGAA CCACTACAGG ACCTAAAAGA GGGCATGGAC
    CAATTAGAAA AGAATAAAAC GTTACGCTCT ATCCTTTCGA CTCTGCTAGC TATTGGCAAC
    TTTCTCAATG GGACCAATGC TAAAGGCTTT GAGTTGAGTT ATCTAGAGAA AGTTCCTGAA
    GTGAAGGACA CGGTTCACAA ACAGTCTCTA CTGCATCATG TCTGCTCGAT TGTTGTGGAG
    AAGTTTCCAG AGAGCACAGA TCTTTACTCT GAGATTGGAG CCATTACTCG CTCTGCTAAG
    GTTGACTTTG ATCAGCTTCA GGAAAACCTG TGTCAAATGG AACGGCGCTG CAAAGCATCC
    TGGGACCACC TGAAGGTCAT CTCCAAACAC GAGATTAAAC CAGCCCTTAA ACAGCGAATG
    TCCGACTTCC TCAAAGACTG TGCAGGAAGA ATTATCATCC TTAAAATTGT CCATCGTCGT
    ATCATAAACA GGTTTCACTC ATTCCTGCTA TTTCTGGGCC ACCCACCATA CGCCATTAGA
    GAAGTGAGCG TCCACCGCTT CAGCAAGATT CTGAGTGAGT TTGCTCTTGA GTACCGAACC
    ACCAGAGAGC GAGTGCTGCA GCAGAAACAG AAACGAGCCA ATCATCGTGA GAGGAACAAG
    ACCCGCGGCA AAATGATCAC AGAGAGTGGG AAGTTTGCAG GAAGTCCTAT GGAGGGTCAG
    GAGTGTCAAC CGCAGGGTTT GGGCTACACA GAGGACGTGA CTGAGCATGA GCAGATGACC
    GCAGTGCTTT GTACCAGCCT GCAGGGTGGC GATAGTCCAT CATCTGTCCT TCCTGGCCTT
    CGCACACGGA CCCGAGCCAC TCGAGGTCAT ACAGCACTTT TGTCCACTGC AAATGAAGAC
    ACTTCTAATT GCACAAATGA CACAGCAGAT GAGATCATGG AGCGGATTGT GAAATCAGCC
    ACTCAGGGCC CCAGTCAGAG AACACAGCCT CGTGAGAGGA GACGTTCCAG AGCAAACCGC
    AAATCCTTGA GAAGAACACT GAAGAACGGT CTTACCCCGG AGGAGGCACA GGCTCTGGGT
    TTGGCCAGCG GCTCTGAGAT GCAGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005158006.1
    CDS398..5047
    Translation

    Target ORF information:

    RefSeq Version XM_005157949.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio formin homology 2 domain containing 3b (fhod3b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005157949.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGCTACGT TTGCATGCAG GGTGCAGTTC TTAGATGATA CAGATCCATT TAATAGCACA 
    AATTTTCCGG AGCCGACCAG ACCCCCACAT TTCACATTCA GAGAGGATAT TCCGCTCATC
    AATCAGATCG CCGGAGTTCA CAGACTTCTC AAAGCACCTC ACAAGCTTGA TGACTGTGCT
    TTGCAGTTGT CTCATAATGG CAGTTATCTG GACCTGGAGT CCACCTTGGC TGAGCAGAAA
    GATGAACTTG AAGGCTTTCA GGAGGATGGA GGGAGAGGAA AGAAGCACAG TATTGTACTT
    AGAACACAGC TGTCTGTCAG AGTCCATGCC TGCATAGAAA AGCTGTACAA CTCGACGGGT
    CGTGAGCTAC GAAGAGCACT GTTCTCTCTC AAGCAAATCT TCCAGGATGA TAAGGATCTG
    GTTCATGAGT TTGTGATGGC GGAGGGACTC ACATGTCTTA TTAAAGTGGG AGCTGAAGCT
    GATCAAAACT ACCAAAACTA CATTCTCAGA GCACTTGGAC AGATCATGCT GTATGTAGAT
    GGAATGAATG GGCTTATCGG ACACAATGAA ACCGTACAGT GGCTGTACAC ACTGGTTGGA
    TCAAAGTTCC GGTTGGTTGT GAAAACTGCT ATGAAGCTGC TGCTGGTTTT TGTGGAGTAC
    ACCGAGAGCA ACGCACCTCT CCTCATCCAA GCCGTCAACA GCGTTGACAC CAAACAAGGC
    TCTAAGCCCT GGTCTAATGC CATGGAAATC CTTGACGAGA AAGATGGAAT GGACACTGAG
    CTGCTGGTTT ATGCCATGAC ACTAATCAAC AAGACATTAG CTGGTCTGCC TGACCAGGAC
    TCCTTCTATG ATGTGGTTGA CAGTCTGGAG GAGCAGAACA TGGAGGCTGT TGCCCAAAGA
    CACATTAGTA GAAAGGGCAC AGATCTGGAC TTGGTAGAGC AGTTCAACAT TTATGAGGCA
    ACTCTTCGTC ATGAAGACGG TGATGATGAA GCTCAGTCTC CTCCATCTGG ACATAAATGT
    CGGCGTCGGG CTAGTGTTGG AGCAACACCT GAGAGACGGG GTTTGGAACG ACGGCGTAGC
    CGAAGACATT CACTCCAGAA CGGAAAAGGC CACACTTCTG CTCCAACTTC CCCCTGTCAC
    AGCCATACAC CCAACATCCT GTCCTTTAAC AGCCAGAGGA TAGAAGACAT CAGTGAAAGA
    GAGGATGAGG AAGAGGAGGA GGAAAAGGAT GAAGAGTTTC AGAAAGCAGC ACTTTCTAGA
    CAGGCATCTG GAAGTTTGTC AGATGTTAGT TACCCAAACA CACCATGCAG TTCTTGTGGC
    AGCAATGGCA ATGCAAGCAC ATATCCCGGC AGGTCATCTG GGGTGTCCAG TGCCCTCTCC
    ACACCCTACA ACCGCTTCGG TCCTTCAGGA GGTCCATCTG CAGACAGTAA AGCCGACAGG
    CCAACTCTGG GTGGATTGTT GACCTCCTCC TACAGGCAGC ACCAAGAATC TCTGAACGCT
    GAAAGAGAGC GGAGACGTCT GGAGAGAGAA GAGAGACTTC AACGGATTGA AAGAGAGGAG
    AGAAACCGCT TCAATCGGGA TTATGCAGAC AAGAGAGAAG AGGTCAGACA TGCCCGTGAA
    GAAAGATACA AGCAAGTGGA GCGTCTGACA GCAGAGGAAA AAGAAAGGGA ACGGCCGAGA
    ACACGTGGTA GAACAGAAAT CACCCTCAGT CTGAGTTCAC GCACTTCCAG CAGCTCCTTG
    TCTCGTTCCG TCACCCCTTT GACTGTAGAT GCTCAAGAGG AGACACGCCC TGTCTCACAT
    ACAGCACAAG GAACTGTGAC TGAGAAAAGT TGTACGGTGC AGACAGCAGT TGTGCGTTCT
    TTATCAGAAC CTCCCTCTGC ATCTCCACCA GTGTCCCCAT CGCAACCAGA GGAGCAACAG
    AGACCTGTCG ACCCATCCGA CATCATTCTA TCTCTATCTC CAACAAACTC TGAACCAGAA
    AGAACAGAGA AAGGGGAGAA AAAAGAGGAT GCTGAAGCAG AGGAGTGTGA GGAAACAGTG
    GTGGGTGAAG TGCAAGATGA AAAGAAGGAA GAACCAGAAG GAAACTTTGA GGATGAGACT
    AATGTGACGG GTGCAGAAGA CTGTGAGAGG GAGAAGACGT TGGATAGAGA AGTAGAGGAG
    AGTGCCATCC TAAGTGAAAA GGAGAAGCAG AATGAAGAAC TGAATGAGAA AGATAACTGC
    TCTGCCTCCA GCATCTCTTC AGCCAGCAGC ACTCTGGAGA GAGAAGAAAG AGAGGAGAAA
    CATGTCAGTG ACAGCAACTC AGGTCAATGG GCAGACTGTA TAAATACAGC AGACAACAAC
    GATCAGTGCA ACAAAGTTCT TAACAGCAAA CGTTTCATGC TGGACATGCT GTATGCCCAA
    AACAAGGAAG GAGAGGAAGA TGAGAAAGAA AAGGAGAACG AAAATGAGAA ATGTTTAGGT
    CAGGACTCTA CTGTGACAAG CCTTGCTAGC CGAATTTCCA CTCTGGAATC ACATCGGCAG
    ACATGTGAGG ACAACTTGAA GAAACTGGAA GTGGAGCATC TGGAAAACCA GGGCAGCGTG
    CGGGCTGTAG CTGAGAAATT TGGAGATTTG GTCAAAGGCA TGTTATCACC TCATGACTTG
    GAGAATTGTG GGAAGGAAAA GCAGCAAACA CAGGAAAAGC CTCCACCTGC TCCATCACCA
    GCACCCCCAA AAAAGGAGTC GGACCACATA TGGGACCAGC TAATGGCAAC GCCACGGGAG
    CTTCGTATCA AAGAGCTGGA TTTCACAGAC TTGGGAGAGG AGGACGATGT GGATATTTTG
    GACGTGGGGA ACATGATTGG GTCAGGTGAC CTCACTCCAC CTCCTCCACC ACCGCCCTGC
    TTGCCCAACC TTCCTCCCCC GCCACCTTTA TTTGGATGCC CTCTGCCTCC CCCAGTCCCT
    GGCATGATGA TGCCCCCTCC ACCACCCTTT TTGGCGTCAA AACCTCCAGC ACAATTGGGA
    TCTCCTCAGC TTAGTCGAGG AGAACCACCG CTCTTCCAAA AGAAGAAGAA GACCATCAGG
    CTATTCTGGA ATGAGGTGAG ACCGATTGAC TGGCAGTACA CCAACCACAA GAGATGCAAG
    GAGTCACTCT GGTCCAAACT GGATCCCATC AAACTGGACA CAGCCAAATT GGAAAACCTC
    TTTGAGACGA AATCTAAGGA GCTGCCAGTT ACAAAGAAAA CAGCAGCAGA TGGTAAGCGT
    CAGGAGATCA TCATTCTGGA TTCCAAAAGA AGTAATGCTA TCAACATTGG CCTGACGGTC
    CTGCCGCCTC CTCGCACCAT CAAAACAGCC ATCCTCAACT TTGATGAGTA CGCCTTGAGC
    AAAGAGGGCA TAGAGAAAAT CCTCACCATG ATTCCTACGG AGGAGGAGAC ACAGAAGATT
    CAGGAGGCCC AGCTGGCGAA CCCCGACACT CCGCTGGGCA GCGCAGAGCA GTTCCTCCTC
    ATTTTGTCCT CCATCAGTGA GCTGTCCGCC CGCCTGCAAC TCTGGGCCTT TAAGATGGAC
    TATGATGCTT TAGAGAAGGA AGTAGCAGAA CCACTACAGG ACCTAAAAGA GGGCATGGAC
    CAATTAGAAA AGAATAAAAC GTTACGCTCT ATCCTTTCGA CTCTGCTAGC TATTGGCAAC
    TTTCTCAATG GGACCAATGC TAAAGGCTTT GAGTTGAGTT ATCTAGAGAA AGTTCCTGAA
    GTGAAGGACA CGGTTCACAA ACAGTCTCTA CTGCATCATG TCTGCTCGAT TGTTGTGGAG
    AAGTTTCCAG AGAGCACAGA TCTTTACTCT GAGATTGGAG CCATTACTCG CTCTGCTAAG
    GTTGACTTTG ATCAGCTTCA GGAAAACCTG TGTCAAATGG AACGGCGCTG CAAAGCATCC
    TGGGACCACC TGAAGGTCAT CTCCAAACAC GAGATTAAAC CAGCCCTTAA ACAGCGAATG
    TCCGACTTCC TCAAAGACTG TGCAGGAAGA ATTATCATCC TTAAAATTGT CCATCGTCGT
    ATCATAAACA GGTTTCACTC ATTCCTGCTA TTTCTGGGCC ACCCACCATA CGCCATTAGA
    GAAGTGAGCG TCCACCGCTT CAGCAAGATT CTGAGTGAGT TTGCTCTTGA GTACCGAACC
    ACCAGAGAGC GAGTGCTGCA GCAGAAACAG AAACGAGCCA ATCATCGTGA GAGGAACAAG
    ACCCGCGGCA AAATGATCAC AGAGAGTGGG AAGTTTGCAG GAAGTCCTAT GGAGGGTCAG
    GAGTGTCAAC CGCAGGGTTT GGGCTACACA GAGGACGTGA CTGAGCATGA GCAGATGACC
    GCAGTGCTTT GTACCAGCCT GCAGGGTGGC GATAGTCCAT CATCTGTCCT TCCTGGCCTT
    CGCACACGGA CCCGAGCCAC TCGAGGTCAT ACAGCACTTT TGTCCACTGC AAATGAAGAC
    ACTTCTAATT GCACAAATGA CACAGCAGAT GAGATCATGG AGCGGATTGT GAAATCAGCC
    ACTCAGGGCC CCAGTCAGAG AACACAGCCT CGTGAGAGGA GACGTTCCAG AGCAAACCGC
    AAATCCTTGA GAAGAACACT GAAGAACGGT CTTACCCCGG AGGAGGCACA GGCTCTGGGT
    TTGGCCAGCG GCTCTGAGAT GCAGGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa21319
    Clone ID Related Accession (Same CDS sequence) XM_005157951.3
    Accession Version XM_005157951.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4527bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product FH1/FH2 domain-containing protein 3 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157951.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTACGT TTGCATGCAG GGTGCAGTTC TTAGATGATA CAGATCCATT TAATAGCACA 
    AATTTTCCGG AGCCGACCAG ACCCCCACAT TTCACATTCA GAGAGGATAT TCCGCTCATC
    AATCAGATCG CCGGAGTTCA CAGACTTCTC AAAGCACCTC ACAAGCTTGA TGACTGTGCT
    TTGCAGTTGT CTCATAATGG CAGTTATCTG GACCTGGAGT CCACCTTGGC TGAGCAGAAA
    GATGAACTTG AAGGCTTTCA GGAGGATGGA GGGAGAGGAA AGAAGCACAG TATTGTACTT
    AGAACACAGC TGTCTGTCAG AGTCCATGCC TGCATAGAAA AGCTGTACAA CTCGACGGGT
    CGTGAGCTAC GAAGAGCACT GTTCTCTCTC AAGCAAATCT TCCAGGATGA TAAGGATCTG
    GTTCATGAGT TTGTGATGGC GGAGGGACTC ACATGTCTTA TTAAAGTGGG AGCTGAAGCT
    GATCAAAACT ACCAAAACTA CATTCTCAGA GCACTTGGAC AGATCATGCT GTATGTAGAT
    GGAATGAATG GGCTTATCGG ACACAATGAA ACCGTACAGT GGCTGTACAC ACTGGTTGGA
    TCAAAGTTCC GGTTGGTTGT GAAAACTGCT ATGAAGCTGC TGCTGGTTTT TGTGGAGTAC
    ACCGAGAGCA ACGCACCTCT CCTCATCCAA GCCGTCAACA GCGTTGACAC CAAACAAGGC
    TCTAAGCCCT GGTCTAATGC CATGGAAATC CTTGACGAGA AAGATGGAAT GGACACTGAG
    CTGCTGGTTT ATGCCATGAC ACTAATCAAC AAGACATTAG CTGGTCTGCC TGACCAGGAC
    TCCTTCTATG ATGTGGTTGA CAGTCTGGAG GAGCAGAACA TGGAGGCTGT TGCCCAAAGA
    CACATTAGTA GAAAGGGCAC AGATCTGGAC TTGGTAGAGC AGTTCAACAT TTATGAGGCA
    ACTCTTCGTC ATGAAGACGG TGATGATGAA GCTCAGTCTC CTCCATCTGG ACATAAATGT
    CGGCGTCGGG CTAGTGTTGG AGCAACACCT GAGAGACGGG GTTTGGAACG ACGGCGTAGC
    CGAAGACATT CACTCCAGAA CGGAAAAGGC CACACTTCTG CTCCAACTTC CCCCTGTCAC
    AGCCATACAC CCAACATCCT GTCCTTTAAC AGCCAGAGGA TAGAAGACAT CAGTGAAAGC
    AATGGCAATG CAAGCACATA TCCCGGCAGG TCATCTGGGG TGTCCAGTGC CCTCTCCACA
    CCCTACAACC GCTTCGGTCC TTCAGGAGGT CCATCTGCAG ACAGTAAAGC CGACAGGCCA
    ACTCTGGGTG GATTGTTGAC CTCCTCCTAC AGGCAGCACC AAGAATCTCT GAACGCTGAA
    AGAGAGCGGA GACGTCTGGA GAGAGAAGAG AGACTTCAAC GGATTGAAAG AGAGGAGAGA
    AACCGCTTCA ATCGGGATTA TGCAGACAAG AGAGAAGAGG TCAGACATGC CCGTGAAGAA
    AGATACAAGC AAGTGGAGCG TCTGACAGCA GAGGAAAAAG AAAGGGAACG GCCGAGAACA
    CGTGGTAGAA CAGAAATCAC CCTCAGTCTG AGTTCACGCA CTTCCAGCAG CTCCTTGTCT
    CGTTCCGTCA CCCCTTTGAC TGTAGATGCT CAAGAGGAGA CACGCCCTGT CTCACATACA
    GCACAAGGAA CTGTGACTGA GAAAAGTTGT ACGGTGCAGA CAGCAGTTGT GCGTTCTTTA
    TCAGAACCTC CCTCTGCATC TCCACCAGTG TCCCCATCGC AACCAGAGGA GCAACAGAGA
    CCTGTCGACC CATCCGACAT CATTCTATCT CTATCTCCAA CAAACTCTGA ACCAGAAAGA
    ACAGAGAAAG GGGAGAAAAA AGAGGATGCT GAAGCAGAGG AGTGTGAGGA AACAGTGGTG
    GGTGAAGTGC AAGATGAAAA GAAGGAAGAA CCAGAAGGAA ACTTTGAGGA TGAGACTAAT
    GTGACGGGTG CAGAAGACTG TGAGAGGGAG AAGACGTTGG ATAGAGAAGT AGAGGAGAGT
    GCCATCCTAA GTGAAAAGGA GAAGCAGAAT GAAGAACTGA ATGAGAAAGA TAACTGCTCT
    GCCTCCAGCA TCTCTTCAGC CAGCAGCACT CTGGAGAGAG AAGAAAGAGA GGAGAAACAT
    GTCAGTGACA GCAACTCAGG TCAATGGGCA GACTGTATAA ATACAGCAGA CAACAACGAT
    CAGTGCAACA AAGTTCTTAA CAGCAAACGT TTCATGCTGG ACATGCTGTA TGCCCAAAAC
    AAGGAAGGAG AGGAAGATGA GAAAGAAAAG GAGAACGAAA ATGAGAAATG TTTAGGTCAG
    GACTCTACTG TGACAAGCCT TGCTAGCCGA ATTTCCACTC TGGAATCACA TCGGCAGACA
    TGTGAGGACA ACTTGAAGAA ACTGGAAGTG GAGCATCTGG AAAACCAGGG CAGCGTGCGG
    GCTGTAGCTG AGAAATTTGG AGATTTGGTC AAAGGCATGT TATCACCTCA TGACTTGGAG
    AATTGTGGGA AGGAAAAGCA GCAAACACAG GAAAAGCCTC CACCTGCTCC ATCACCAGCA
    CCCCCAAAAA AGGAGTCGGA CCACATATGG GACCAGCTAA TGGCAACGCC ACGGGAGCTT
    CGTATCAAAG AGCTGGATTT CACAGACTTG GGAGAGGAGG ACGATGTGGA TATTTTGGAC
    GTGGGGAACA TGATTGGGTC AGGTGACCTC ACTCCACCTC CTCCACCACC GCCCTGCTTG
    CCCAACCTTC CTCCCCCGCC ACCTTTATTT GGATGCCCTC TGCCTCCCCC AGTCCCTGGC
    ATGATGATGC CCCCTCCACC ACCCTTTTTG GCGTCAAAAC CTCCAGCACA ATTGGGATCT
    CCTCAGCTTA GTCGAGGAGA ACCACCGCTC TTCCAAAAGA AGAAGAAGAC CATCAGGCTA
    TTCTGGAATG AGGTGAGACC GATTGACTGG CAGTACACCA ACCACAAGAG ATGCAAGGAG
    TCACTCTGGT CCAAACTGGA TCCCATCAAA CTGGACACAG CCAAATTGGA AAACCTCTTT
    GAGACGAAAT CTAAGGAGCT GCCAGTTACA AAGAAAACAG CAGCAGATGG TAAGCGTCAG
    GAGATCATCA TTCTGGATTC CAAAAGAAGT AATGCTATCA ACATTGGCCT GACGGTCCTG
    CCGCCTCCTC GCACCATCAA AACAGCCATC CTCAACTTTG ATGAGTACGC CTTGAGCAAA
    GAGGGCATAG AGAAAATCCT CACCATGATT CCTACGGAGG AGGAGACACA GAAGATTCAG
    GAGGCCCAGC TGGCGAACCC CGACACTCCG CTGGGCAGCG CAGAGCAGTT CCTCCTCATT
    TTGTCCTCCA TCAGTGAGCT GTCCGCCCGC CTGCAACTCT GGGCCTTTAA GATGGACTAT
    GATGCTTTAG AGAAGGAAGT AGCAGAACCA CTACAGGACC TAAAAGAGGG CATGGACCAA
    TTAGAAAAGA ATAAAACGTT ACGCTCTATC CTTTCGACTC TGCTAGCTAT TGGCAACTTT
    CTCAATGGGA CCAATGCTAA AGGCTTTGAG TTGAGTTATC TAGAGAAAGT TCCTGAAGTG
    AAGGACACGG TTCACAAACA GTCTCTACTG CATCATGTCT GCTCGATTGT TGTGGAGAAG
    TTTCCAGAGA GCACAGATCT TTACTCTGAG ATTGGAGCCA TTACTCGCTC TGCTAAGGTT
    GACTTTGATC AGCTTCAGGA AAACCTGTGT CAAATGGAAC GGCGCTGCAA AGCATCCTGG
    GACCACCTGA AGGTCATCTC CAAACACGAG ATTAAACCAG CCCTTAAACA GCGAATGTCC
    GACTTCCTCA AAGACTGTGC AGGAAGAATT ATCATCCTTA AAATTGTCCA TCGTCGTATC
    ATAAACAGGT TTCACTCATT CCTGCTATTT CTGGGCCACC CACCATACGC CATTAGAGAA
    GTGAGCGTCC ACCGCTTCAG CAAGATTCTG AGTGAGTTTG CTCTTGAGTA CCGAACCACC
    AGAGAGCGAG TGCTGCAGCA GAAACAGAAA CGAGCCAATC ATCGTGAGAG GAACAAGACC
    CGCGGCAAAA TGATCACAGA GAGTGGGAAG TTTGCAGGAA GTCCTATGGA GGGTCAGGAG
    TGTCAACCGC AGGGTTTGGG CTACACAGAG GACGTGACTG AGCATGAGCA GATGACCGCA
    GTGCTTTGTA CCAGCCTGCA GGGTGGCGAT AGTCCATCAT CTGTCCTTCC TGGCCTTCGC
    ACACGGACCC GAGCCACTCG AGGTCATACA GCACTTTTGT CCACTGCAAA TGAAGACACT
    TCTAATTGCA CAAATGACAC AGCAGATGAG ATCATGGAGC GGATTGTGAA ATCAGCCACT
    CAGGGCCCCA GTCAGAGAAC ACAGCCTCGT GAGAGGAGAC GTTCCAGAGC AAACCGCAAA
    TCCTTGAGAA GAACACTGAA GAACGGTCTT ACCCCGGAGG AGGCACAGGC TCTGGGTTTG
    GCCAGCGGCT CTGAGATGCA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005158008.1
    CDS392..4918
    Translation

    Target ORF information:

    RefSeq Version XM_005157951.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio formin homology 2 domain containing 3b (fhod3b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005157951.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGCTACGT TTGCATGCAG GGTGCAGTTC TTAGATGATA CAGATCCATT TAATAGCACA 
    AATTTTCCGG AGCCGACCAG ACCCCCACAT TTCACATTCA GAGAGGATAT TCCGCTCATC
    AATCAGATCG CCGGAGTTCA CAGACTTCTC AAAGCACCTC ACAAGCTTGA TGACTGTGCT
    TTGCAGTTGT CTCATAATGG CAGTTATCTG GACCTGGAGT CCACCTTGGC TGAGCAGAAA
    GATGAACTTG AAGGCTTTCA GGAGGATGGA GGGAGAGGAA AGAAGCACAG TATTGTACTT
    AGAACACAGC TGTCTGTCAG AGTCCATGCC TGCATAGAAA AGCTGTACAA CTCGACGGGT
    CGTGAGCTAC GAAGAGCACT GTTCTCTCTC AAGCAAATCT TCCAGGATGA TAAGGATCTG
    GTTCATGAGT TTGTGATGGC GGAGGGACTC ACATGTCTTA TTAAAGTGGG AGCTGAAGCT
    GATCAAAACT ACCAAAACTA CATTCTCAGA GCACTTGGAC AGATCATGCT GTATGTAGAT
    GGAATGAATG GGCTTATCGG ACACAATGAA ACCGTACAGT GGCTGTACAC ACTGGTTGGA
    TCAAAGTTCC GGTTGGTTGT GAAAACTGCT ATGAAGCTGC TGCTGGTTTT TGTGGAGTAC
    ACCGAGAGCA ACGCACCTCT CCTCATCCAA GCCGTCAACA GCGTTGACAC CAAACAAGGC
    TCTAAGCCCT GGTCTAATGC CATGGAAATC CTTGACGAGA AAGATGGAAT GGACACTGAG
    CTGCTGGTTT ATGCCATGAC ACTAATCAAC AAGACATTAG CTGGTCTGCC TGACCAGGAC
    TCCTTCTATG ATGTGGTTGA CAGTCTGGAG GAGCAGAACA TGGAGGCTGT TGCCCAAAGA
    CACATTAGTA GAAAGGGCAC AGATCTGGAC TTGGTAGAGC AGTTCAACAT TTATGAGGCA
    ACTCTTCGTC ATGAAGACGG TGATGATGAA GCTCAGTCTC CTCCATCTGG ACATAAATGT
    CGGCGTCGGG CTAGTGTTGG AGCAACACCT GAGAGACGGG GTTTGGAACG ACGGCGTAGC
    CGAAGACATT CACTCCAGAA CGGAAAAGGC CACACTTCTG CTCCAACTTC CCCCTGTCAC
    AGCCATACAC CCAACATCCT GTCCTTTAAC AGCCAGAGGA TAGAAGACAT CAGTGAAAGC
    AATGGCAATG CAAGCACATA TCCCGGCAGG TCATCTGGGG TGTCCAGTGC CCTCTCCACA
    CCCTACAACC GCTTCGGTCC TTCAGGAGGT CCATCTGCAG ACAGTAAAGC CGACAGGCCA
    ACTCTGGGTG GATTGTTGAC CTCCTCCTAC AGGCAGCACC AAGAATCTCT GAACGCTGAA
    AGAGAGCGGA GACGTCTGGA GAGAGAAGAG AGACTTCAAC GGATTGAAAG AGAGGAGAGA
    AACCGCTTCA ATCGGGATTA TGCAGACAAG AGAGAAGAGG TCAGACATGC CCGTGAAGAA
    AGATACAAGC AAGTGGAGCG TCTGACAGCA GAGGAAAAAG AAAGGGAACG GCCGAGAACA
    CGTGGTAGAA CAGAAATCAC CCTCAGTCTG AGTTCACGCA CTTCCAGCAG CTCCTTGTCT
    CGTTCCGTCA CCCCTTTGAC TGTAGATGCT CAAGAGGAGA CACGCCCTGT CTCACATACA
    GCACAAGGAA CTGTGACTGA GAAAAGTTGT ACGGTGCAGA CAGCAGTTGT GCGTTCTTTA
    TCAGAACCTC CCTCTGCATC TCCACCAGTG TCCCCATCGC AACCAGAGGA GCAACAGAGA
    CCTGTCGACC CATCCGACAT CATTCTATCT CTATCTCCAA CAAACTCTGA ACCAGAAAGA
    ACAGAGAAAG GGGAGAAAAA AGAGGATGCT GAAGCAGAGG AGTGTGAGGA AACAGTGGTG
    GGTGAAGTGC AAGATGAAAA GAAGGAAGAA CCAGAAGGAA ACTTTGAGGA TGAGACTAAT
    GTGACGGGTG CAGAAGACTG TGAGAGGGAG AAGACGTTGG ATAGAGAAGT AGAGGAGAGT
    GCCATCCTAA GTGAAAAGGA GAAGCAGAAT GAAGAACTGA ATGAGAAAGA TAACTGCTCT
    GCCTCCAGCA TCTCTTCAGC CAGCAGCACT CTGGAGAGAG AAGAAAGAGA GGAGAAACAT
    GTCAGTGACA GCAACTCAGG TCAATGGGCA GACTGTATAA ATACAGCAGA CAACAACGAT
    CAGTGCAACA AAGTTCTTAA CAGCAAACGT TTCATGCTGG ACATGCTGTA TGCCCAAAAC
    AAGGAAGGAG AGGAAGATGA GAAAGAAAAG GAGAACGAAA ATGAGAAATG TTTAGGTCAG
    GACTCTACTG TGACAAGCCT TGCTAGCCGA ATTTCCACTC TGGAATCACA TCGGCAGACA
    TGTGAGGACA ACTTGAAGAA ACTGGAAGTG GAGCATCTGG AAAACCAGGG CAGCGTGCGG
    GCTGTAGCTG AGAAATTTGG AGATTTGGTC AAAGGCATGT TATCACCTCA TGACTTGGAG
    AATTGTGGGA AGGAAAAGCA GCAAACACAG GAAAAGCCTC CACCTGCTCC ATCACCAGCA
    CCCCCAAAAA AGGAGTCGGA CCACATATGG GACCAGCTAA TGGCAACGCC ACGGGAGCTT
    CGTATCAAAG AGCTGGATTT CACAGACTTG GGAGAGGAGG ACGATGTGGA TATTTTGGAC
    GTGGGGAACA TGATTGGGTC AGGTGACCTC ACTCCACCTC CTCCACCACC GCCCTGCTTG
    CCCAACCTTC CTCCCCCGCC ACCTTTATTT GGATGCCCTC TGCCTCCCCC AGTCCCTGGC
    ATGATGATGC CCCCTCCACC ACCCTTTTTG GCGTCAAAAC CTCCAGCACA ATTGGGATCT
    CCTCAGCTTA GTCGAGGAGA ACCACCGCTC TTCCAAAAGA AGAAGAAGAC CATCAGGCTA
    TTCTGGAATG AGGTGAGACC GATTGACTGG CAGTACACCA ACCACAAGAG ATGCAAGGAG
    TCACTCTGGT CCAAACTGGA TCCCATCAAA CTGGACACAG CCAAATTGGA AAACCTCTTT
    GAGACGAAAT CTAAGGAGCT GCCAGTTACA AAGAAAACAG CAGCAGATGG TAAGCGTCAG
    GAGATCATCA TTCTGGATTC CAAAAGAAGT AATGCTATCA ACATTGGCCT GACGGTCCTG
    CCGCCTCCTC GCACCATCAA AACAGCCATC CTCAACTTTG ATGAGTACGC CTTGAGCAAA
    GAGGGCATAG AGAAAATCCT CACCATGATT CCTACGGAGG AGGAGACACA GAAGATTCAG
    GAGGCCCAGC TGGCGAACCC CGACACTCCG CTGGGCAGCG CAGAGCAGTT CCTCCTCATT
    TTGTCCTCCA TCAGTGAGCT GTCCGCCCGC CTGCAACTCT GGGCCTTTAA GATGGACTAT
    GATGCTTTAG AGAAGGAAGT AGCAGAACCA CTACAGGACC TAAAAGAGGG CATGGACCAA
    TTAGAAAAGA ATAAAACGTT ACGCTCTATC CTTTCGACTC TGCTAGCTAT TGGCAACTTT
    CTCAATGGGA CCAATGCTAA AGGCTTTGAG TTGAGTTATC TAGAGAAAGT TCCTGAAGTG
    AAGGACACGG TTCACAAACA GTCTCTACTG CATCATGTCT GCTCGATTGT TGTGGAGAAG
    TTTCCAGAGA GCACAGATCT TTACTCTGAG ATTGGAGCCA TTACTCGCTC TGCTAAGGTT
    GACTTTGATC AGCTTCAGGA AAACCTGTGT CAAATGGAAC GGCGCTGCAA AGCATCCTGG
    GACCACCTGA AGGTCATCTC CAAACACGAG ATTAAACCAG CCCTTAAACA GCGAATGTCC
    GACTTCCTCA AAGACTGTGC AGGAAGAATT ATCATCCTTA AAATTGTCCA TCGTCGTATC
    ATAAACAGGT TTCACTCATT CCTGCTATTT CTGGGCCACC CACCATACGC CATTAGAGAA
    GTGAGCGTCC ACCGCTTCAG CAAGATTCTG AGTGAGTTTG CTCTTGAGTA CCGAACCACC
    AGAGAGCGAG TGCTGCAGCA GAAACAGAAA CGAGCCAATC ATCGTGAGAG GAACAAGACC
    CGCGGCAAAA TGATCACAGA GAGTGGGAAG TTTGCAGGAA GTCCTATGGA GGGTCAGGAG
    TGTCAACCGC AGGGTTTGGG CTACACAGAG GACGTGACTG AGCATGAGCA GATGACCGCA
    GTGCTTTGTA CCAGCCTGCA GGGTGGCGAT AGTCCATCAT CTGTCCTTCC TGGCCTTCGC
    ACACGGACCC GAGCCACTCG AGGTCATACA GCACTTTTGT CCACTGCAAA TGAAGACACT
    TCTAATTGCA CAAATGACAC AGCAGATGAG ATCATGGAGC GGATTGTGAA ATCAGCCACT
    CAGGGCCCCA GTCAGAGAAC ACAGCCTCGT GAGAGGAGAC GTTCCAGAGC AAACCGCAAA
    TCCTTGAGAA GAACACTGAA GAACGGTCTT ACCCCGGAGG AGGCACAGGC TCTGGGTTTG
    GCCAGCGGCT CTGAGATGCA GGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46042
    Clone ID Related Accession (Same CDS sequence) XM_009292259.2
    Accession Version XM_009292259.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4764bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product FH1/FH2 domain-containing protein 3 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292259.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCTACGT TTGCATGCAG GGTGCAGTTC TTAGATGATA CAGATCCATT TAATAGCACA 
    AATTTTCCGG AGCCGACCAG ACCCCCACAT TTCACATTCA GAGAGGATAT TCCGCTCATC
    AATCAGATCG CCGGAGTTCA CAGACTTCTC AAAGCACCTC ACAAGCTTGA TGACTGTGCT
    TTGCAGTTGT CTCATAATGG CAGTTATCTG GACCTGGAGT CCACCTTGGC TGAGCAGAAA
    GATGAACTTG AAGGCTTTCA GGAGGATGGA GGGAGAGGAA AGAAGCACAG TATTGTACTT
    AGAACACAGC TGTCTGTCAG AGTCCATGCC TGCATAGAAA AGCTGTACAA CTCGACGGGT
    CGTGAGCTAC GAAGAGCACT GTTCTCTCTC AAGCAAATCT TCCAGGATGA TAAGGATCTG
    GTTCATGAGT TTGTGATGGC GGAGGGACTC ACATGTCTTA TTAAAGTGGG AGCTGAAGCT
    GATCAAAACT ACCAAAACTA CATTCTCAGA GCACTTGGAC AGATCATGCT GTATGTAGAT
    GGAATGAATG GGCTTATCGG ACACAATGAA ACCGTACAGT GGCTGTACAC ACTGGTTGGA
    TCAAAGTTCC GGTTGGTTGT GAAAACTGCT ATGAAGCTGC TGCTGGTTTT TGTGGAGTAC
    ACCGAGAGCA ACGCACCTCT CCTCATCCAA GCCGTCAACA GCGTTGACAC CAAACAAGGC
    TCTAAGCCCT GGTCTAATGC CATGGAAATC CTTGACGAGA AAGATGGAAT GGACACTGAG
    CTGCTGGTTT ATGCCATGAC ACTAATCAAC AAGACATTAG CTGGTCTGCC TGACCAGGAC
    TCCTTCTATG ATGTGGTTGA CAGTCTGGAG GAGCAGAACA TGGAGGCTGT TGCCCAAAGA
    CACATTAGTA GAAAGGGCAC AGATCTGGAC TTGGTAGAGC AGTTCAACAT TTATGAGGCA
    ACTCTTCGTC ATGAAGACGG TGATGATGAA GCTCAGTCTC CTCCATCTGG ACATAAATGT
    CGGCGTCGGG CTAGTGTTGG AGCAACACCT GAGAGACGGG GTTTGGAACG ACGGCGTAGC
    CGAAGACATT CACTCCAGAA CGGAAAAGGC CACACTTCTG CTCCAACTTC CCCCTGTCAC
    AGCCATACAC CCAACATCCT GTCCTTTAAC AGCCAGAGGA TAGAAGACAT CAGTGAAAGA
    GAGGATGAGG AAGAGGAGGA GGAAAAGGAT GAAGAGTTTC AGAAAGCAGC ACTTTCTAGA
    CAGGCATCTG GAAGCTGTGT GGCATCAACA GTAGAGGACG CTTCTAGCTT GTGTTTGCCT
    TTAAGGTGCA TGGTCAACTT CATTCCAAAT GCTGCTGACA CAGAAAATGC AGAAAAACCT
    CGGAACAGTT TGTCAGATGT TAGTTACCCA AACACACCAT GCAGTTCTTG TGGCAGCAAT
    GGCAATGCAA GCACATATCC CGGCAGGTCA TCTGGGGTGT CCAGTGCCCT CTCCACACCC
    TACAACCGCT TCGGTCCTTC AGGAGGTCCA TCTGCAGACA GTAAAGCCGA CAGGCCAACT
    CTGGGTGGAT TGTTGACCTC CTCCTACAGG CAGCACCAAG AATCTCTGAA CGCTGAAAGA
    GAGCGGAGAC GTCTGGAGAG AGAAGAGAGA CTTCAACGGA TTGAAAGAGA GGAGAGAAAC
    CGCTTCAATC GGGATTATGC AGACAAGAGA GAAGAGGTCA GACATGCCCG TGAAGAAAGA
    TACAAGCAAG TGGAGCGTCT GACAGCAGAG GAAAAAGAAA GGGAACGGCC GAGAACACGT
    GGTAGAACAG AAATCACCCT CAGTCTGAGT TCACGCACTT CCAGCAGCTC CTTGTCTCGT
    TCCGTCACCC CTTTGACTGT AGATGCTCAA GAGGAGACAC GCCCTGTCTC ACATACAGCA
    CAAGGAACTG TGACTGAGAA AAGTTGTACG GTGCAGACAG CAGTTGTGCG TTCTTTATCA
    GAACCTCCCT CTGCATCTCC ACCAGTGTCC CCATCGCAAC CAGAGGAGCA ACAGAGACCT
    GTCGACCCAT CCGACATCAT TCTATCTCTA TCTCCAACAA ACTCTGAACC AGAAAGAACA
    GAGAAAGGGG AGAAAAAAGA GGATGCTGAA GCAGAGGAGT GTGAGGAAAC AGTGGTGGGT
    GAAGTGCAAG ATGAAAAGAA GGAAGAACCA GAAGGAAACT TTGAGGATGA GACTAATGTG
    ACGGGTGCAG AAGACTGTGA GAGGGAGAAG ACGTTGGATA GAGAAGTAGA GGAGAGTGCC
    ATCCTAAGTG AAAAGGAGAA GCAGAATGAA GAACTGAATG AGAAAGATAA CTGCTCTGCC
    TCCAGCATCT CTTCAGCCAG CAGCACTCTG GAGAGAGAAG AAAGAGAGGA GAAACATGTC
    AGTGACAGCA ACTCAGGTCA ATGGGCAGAC TGTATAAATA CAGCAGACAA CAACGATCAG
    TGCAACAAAG TTCTTAACAG CAAACGTTTC ATGCTGGACA TGCTGTATGC CCAAAACAAG
    GAAGGAGAGG AAGATGAGAA AGAAAAGGAG AACGAAAATG AGAAATGTTT AGGTCAGGAC
    TCTACTGTGA CAAGCCTTGC TAGCCGAATT TCCACTCTGG AATCACATCG GCAGACATGT
    GAGGACAACT TGAAGAAACT GGAAGTGGAG CATCTGGAAA ACCAGGGCAG CGTGCGGGCT
    GTAGCTGAGA AATTTGGAGA TTTGGTCAAA GGCATGTTAT CACCTCATGA CTTGGAGAAT
    TGTGGGAAGG AAAAGCAGCA AACACAGGAA AAGCCTCCAC CTGCTCCATC ACCAGCACCC
    CCAAAAAAGG AGTCGGACCA CATATGGGAC CAGCTAATGG CAACGCCACG GGAGCTTCGT
    ATCAAAGAGC TGGATTTCAC AGACTTGGGA GAGGAGGACG ATGTGGATAT TTTGGACGTG
    GGGAACATGA TTGGGTCAGG TGACCTCACT CCACCTCCTC CACCACCGCC CTGCTTGCCC
    AACCTTCCTC CCCCGCCACC TTTATTTGGA TGCCCTCTGC CTCCCCCAGT CCCTGGCATG
    ATGATGCCCC CTCCACCACC CTTTTTGGCG TCAAAACCTC CAGCACAATT GGGATCTCCT
    CAGCTTAGTC GAGGAGAACC ACCGCTCTTC CAAAAGAAGA AGAAGACCAT CAGGCTATTC
    TGGAATGAGG TGAGACCGAT TGACTGGCAG TACACCAACC ACAAGAGATG CAAGGAGTCA
    CTCTGGTCCA AACTGGATCC CATCAAACTG GACACAGCCA AATTGGAAAA CCTCTTTGAG
    ACGAAATCTA AGGAGCTGCC AGTTACAAAG AAAACAGCAG CAGATGGTAA GCGTCAGGAG
    ATCATCATTC TGGATTCCAA AAGAAGTAAT GCTATCAACA TTGGCCTGAC GGTCCTGCCG
    CCTCCTCGCA CCATCAAAAC AGCCATCCTC AACTTTGATG AGTACGCCTT GAGCAAAGAG
    GGCATAGAGA AAATCCTCAC CATGATTCCT ACGGAGGAGG AGACACAGAA GATTCAGGAG
    GCCCAGCTGG CGAACCCCGA CACTCCGCTG GGCAGCGCAG AGCAGTTCCT CCTCATTTTG
    TCCTCCATCA GTGAGCTGTC CGCCCGCCTG CAACTCTGGG CCTTTAAGAT GGACTATGAT
    GCTTTAGAGA AGGAAGTAGC AGAACCACTA CAGGACCTAA AAGAGGGCAT GGACCAATTA
    GAAAAGAATA AAACGTTACG CTCTATCCTT TCGACTCTGC TAGCTATTGG CAACTTTCTC
    AATGGGACCA ATGCTAAAGG CTTTGAGTTG AGTTATCTAG AGAAAGTTCC TGAAGTGAAG
    GACACGGTTC ACAAACAGTC TCTACTGCAT CATGTCTGCT CGATTGTTGT GGAGAAGTTT
    CCAGAGAGCA CAGATCTTTA CTCTGAGATT GGAGCCATTA CTCGCTCTGC TAAGGTTGAC
    TTTGATCAGC TTCAGGAAAA CCTGTGTCAA ATGGAACGGC GCTGCAAAGC ATCCTGGGAC
    CACCTGAAGG TCATCTCCAA ACACGAGATT AAACCAGCCC TTAAACAGCG AATGTCCGAC
    TTCCTCAAAG ACTGTGCAGG AAGAATTATC ATCCTTAAAA TTGTCCATCG TCGTATCATA
    AACAGGTTTC ACTCATTCCT GCTATTTCTG GGCCACCCAC CATACGCCAT TAGAGAAGTG
    AGCGTCCACC GCTTCAGCAA GATTCTGAGT GAGTTTGCTC TTGAGTACCG AACCACCAGA
    GAGCGAGTGC TGCAGCAGAA ACAGAAACGA GCCAATCATC GTGAGAGGAA CAAGACCCGC
    GGCAAAATGA TCACAGAGAG TGGGAAGTTT GCAGGAAGTC CTATGGAGGG TCAGGAGTGT
    CAACCGCAGG GTTTGGGCTA CACAGAGGAC GTGACTGAGC ATGAGCAGAT GACCGCAGTG
    CTTTGTACCA GCCTGCAGGG TGGCGATAGT CCATCATCTG TCCTTCCTGG CCTTCGCACA
    CGGACCCGAG CCACTCGAGG TCATACAGCA CTTTTGTCCA CTGCAAATGA AGACACTTCT
    AATTGCACAA ATGACACAGC AGATGAGATC ATGGAGCGGA TTGTGAAATC AGCCACTCAG
    GGCCCCAGTC AGAGAACACA GCCTCGTGAG AGGAGACGTT CCAGAGCAAA CCGCAAATCC
    TTGAGAAGAA CACTGAAGAA CGGTCTTACC CCGGAGGAGG CACAGGCTCT GGGTTTGGCC
    AGCGGCTCTG AGATGCAGGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290534.1
    CDS401..5164
    Translation

    Target ORF information:

    RefSeq Version XM_009292259.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio formin homology 2 domain containing 3b (fhod3b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292259.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGGCTACGT TTGCATGCAG GGTGCAGTTC TTAGATGATA CAGATCCATT TAATAGCACA 
    AATTTTCCGG AGCCGACCAG ACCCCCACAT TTCACATTCA GAGAGGATAT TCCGCTCATC
    AATCAGATCG CCGGAGTTCA CAGACTTCTC AAAGCACCTC ACAAGCTTGA TGACTGTGCT
    TTGCAGTTGT CTCATAATGG CAGTTATCTG GACCTGGAGT CCACCTTGGC TGAGCAGAAA
    GATGAACTTG AAGGCTTTCA GGAGGATGGA GGGAGAGGAA AGAAGCACAG TATTGTACTT
    AGAACACAGC TGTCTGTCAG AGTCCATGCC TGCATAGAAA AGCTGTACAA CTCGACGGGT
    CGTGAGCTAC GAAGAGCACT GTTCTCTCTC AAGCAAATCT TCCAGGATGA TAAGGATCTG
    GTTCATGAGT TTGTGATGGC GGAGGGACTC ACATGTCTTA TTAAAGTGGG AGCTGAAGCT
    GATCAAAACT ACCAAAACTA CATTCTCAGA GCACTTGGAC AGATCATGCT GTATGTAGAT
    GGAATGAATG GGCTTATCGG ACACAATGAA ACCGTACAGT GGCTGTACAC ACTGGTTGGA
    TCAAAGTTCC GGTTGGTTGT GAAAACTGCT ATGAAGCTGC TGCTGGTTTT TGTGGAGTAC
    ACCGAGAGCA ACGCACCTCT CCTCATCCAA GCCGTCAACA GCGTTGACAC CAAACAAGGC
    TCTAAGCCCT GGTCTAATGC CATGGAAATC CTTGACGAGA AAGATGGAAT GGACACTGAG
    CTGCTGGTTT ATGCCATGAC ACTAATCAAC AAGACATTAG CTGGTCTGCC TGACCAGGAC
    TCCTTCTATG ATGTGGTTGA CAGTCTGGAG GAGCAGAACA TGGAGGCTGT TGCCCAAAGA
    CACATTAGTA GAAAGGGCAC AGATCTGGAC TTGGTAGAGC AGTTCAACAT TTATGAGGCA
    ACTCTTCGTC ATGAAGACGG TGATGATGAA GCTCAGTCTC CTCCATCTGG ACATAAATGT
    CGGCGTCGGG CTAGTGTTGG AGCAACACCT GAGAGACGGG GTTTGGAACG ACGGCGTAGC
    CGAAGACATT CACTCCAGAA CGGAAAAGGC CACACTTCTG CTCCAACTTC CCCCTGTCAC
    AGCCATACAC CCAACATCCT GTCCTTTAAC AGCCAGAGGA TAGAAGACAT CAGTGAAAGA
    GAGGATGAGG AAGAGGAGGA GGAAAAGGAT GAAGAGTTTC AGAAAGCAGC ACTTTCTAGA
    CAGGCATCTG GAAGCTGTGT GGCATCAACA GTAGAGGACG CTTCTAGCTT GTGTTTGCCT
    TTAAGGTGCA TGGTCAACTT CATTCCAAAT GCTGCTGACA CAGAAAATGC AGAAAAACCT
    CGGAACAGTT TGTCAGATGT TAGTTACCCA AACACACCAT GCAGTTCTTG TGGCAGCAAT
    GGCAATGCAA GCACATATCC CGGCAGGTCA TCTGGGGTGT CCAGTGCCCT CTCCACACCC
    TACAACCGCT TCGGTCCTTC AGGAGGTCCA TCTGCAGACA GTAAAGCCGA CAGGCCAACT
    CTGGGTGGAT TGTTGACCTC CTCCTACAGG CAGCACCAAG AATCTCTGAA CGCTGAAAGA
    GAGCGGAGAC GTCTGGAGAG AGAAGAGAGA CTTCAACGGA TTGAAAGAGA GGAGAGAAAC
    CGCTTCAATC GGGATTATGC AGACAAGAGA GAAGAGGTCA GACATGCCCG TGAAGAAAGA
    TACAAGCAAG TGGAGCGTCT GACAGCAGAG GAAAAAGAAA GGGAACGGCC GAGAACACGT
    GGTAGAACAG AAATCACCCT CAGTCTGAGT TCACGCACTT CCAGCAGCTC CTTGTCTCGT
    TCCGTCACCC CTTTGACTGT AGATGCTCAA GAGGAGACAC GCCCTGTCTC ACATACAGCA
    CAAGGAACTG TGACTGAGAA AAGTTGTACG GTGCAGACAG CAGTTGTGCG TTCTTTATCA
    GAACCTCCCT CTGCATCTCC ACCAGTGTCC CCATCGCAAC CAGAGGAGCA ACAGAGACCT
    GTCGACCCAT CCGACATCAT TCTATCTCTA TCTCCAACAA ACTCTGAACC AGAAAGAACA
    GAGAAAGGGG AGAAAAAAGA GGATGCTGAA GCAGAGGAGT GTGAGGAAAC AGTGGTGGGT
    GAAGTGCAAG ATGAAAAGAA GGAAGAACCA GAAGGAAACT TTGAGGATGA GACTAATGTG
    ACGGGTGCAG AAGACTGTGA GAGGGAGAAG ACGTTGGATA GAGAAGTAGA GGAGAGTGCC
    ATCCTAAGTG AAAAGGAGAA GCAGAATGAA GAACTGAATG AGAAAGATAA CTGCTCTGCC
    TCCAGCATCT CTTCAGCCAG CAGCACTCTG GAGAGAGAAG AAAGAGAGGA GAAACATGTC
    AGTGACAGCA ACTCAGGTCA ATGGGCAGAC TGTATAAATA CAGCAGACAA CAACGATCAG
    TGCAACAAAG TTCTTAACAG CAAACGTTTC ATGCTGGACA TGCTGTATGC CCAAAACAAG
    GAAGGAGAGG AAGATGAGAA AGAAAAGGAG AACGAAAATG AGAAATGTTT AGGTCAGGAC
    TCTACTGTGA CAAGCCTTGC TAGCCGAATT TCCACTCTGG AATCACATCG GCAGACATGT
    GAGGACAACT TGAAGAAACT GGAAGTGGAG CATCTGGAAA ACCAGGGCAG CGTGCGGGCT
    GTAGCTGAGA AATTTGGAGA TTTGGTCAAA GGCATGTTAT CACCTCATGA CTTGGAGAAT
    TGTGGGAAGG AAAAGCAGCA AACACAGGAA AAGCCTCCAC CTGCTCCATC ACCAGCACCC
    CCAAAAAAGG AGTCGGACCA CATATGGGAC CAGCTAATGG CAACGCCACG GGAGCTTCGT
    ATCAAAGAGC TGGATTTCAC AGACTTGGGA GAGGAGGACG ATGTGGATAT TTTGGACGTG
    GGGAACATGA TTGGGTCAGG TGACCTCACT CCACCTCCTC CACCACCGCC CTGCTTGCCC
    AACCTTCCTC CCCCGCCACC TTTATTTGGA TGCCCTCTGC CTCCCCCAGT CCCTGGCATG
    ATGATGCCCC CTCCACCACC CTTTTTGGCG TCAAAACCTC CAGCACAATT GGGATCTCCT
    CAGCTTAGTC GAGGAGAACC ACCGCTCTTC CAAAAGAAGA AGAAGACCAT CAGGCTATTC
    TGGAATGAGG TGAGACCGAT TGACTGGCAG TACACCAACC ACAAGAGATG CAAGGAGTCA
    CTCTGGTCCA AACTGGATCC CATCAAACTG GACACAGCCA AATTGGAAAA CCTCTTTGAG
    ACGAAATCTA AGGAGCTGCC AGTTACAAAG AAAACAGCAG CAGATGGTAA GCGTCAGGAG
    ATCATCATTC TGGATTCCAA AAGAAGTAAT GCTATCAACA TTGGCCTGAC GGTCCTGCCG
    CCTCCTCGCA CCATCAAAAC AGCCATCCTC AACTTTGATG AGTACGCCTT GAGCAAAGAG
    GGCATAGAGA AAATCCTCAC CATGATTCCT ACGGAGGAGG AGACACAGAA GATTCAGGAG
    GCCCAGCTGG CGAACCCCGA CACTCCGCTG GGCAGCGCAG AGCAGTTCCT CCTCATTTTG
    TCCTCCATCA GTGAGCTGTC CGCCCGCCTG CAACTCTGGG CCTTTAAGAT GGACTATGAT
    GCTTTAGAGA AGGAAGTAGC AGAACCACTA CAGGACCTAA AAGAGGGCAT GGACCAATTA
    GAAAAGAATA AAACGTTACG CTCTATCCTT TCGACTCTGC TAGCTATTGG CAACTTTCTC
    AATGGGACCA ATGCTAAAGG CTTTGAGTTG AGTTATCTAG AGAAAGTTCC TGAAGTGAAG
    GACACGGTTC ACAAACAGTC TCTACTGCAT CATGTCTGCT CGATTGTTGT GGAGAAGTTT
    CCAGAGAGCA CAGATCTTTA CTCTGAGATT GGAGCCATTA CTCGCTCTGC TAAGGTTGAC
    TTTGATCAGC TTCAGGAAAA CCTGTGTCAA ATGGAACGGC GCTGCAAAGC ATCCTGGGAC
    CACCTGAAGG TCATCTCCAA ACACGAGATT AAACCAGCCC TTAAACAGCG AATGTCCGAC
    TTCCTCAAAG ACTGTGCAGG AAGAATTATC ATCCTTAAAA TTGTCCATCG TCGTATCATA
    AACAGGTTTC ACTCATTCCT GCTATTTCTG GGCCACCCAC CATACGCCAT TAGAGAAGTG
    AGCGTCCACC GCTTCAGCAA GATTCTGAGT GAGTTTGCTC TTGAGTACCG AACCACCAGA
    GAGCGAGTGC TGCAGCAGAA ACAGAAACGA GCCAATCATC GTGAGAGGAA CAAGACCCGC
    GGCAAAATGA TCACAGAGAG TGGGAAGTTT GCAGGAAGTC CTATGGAGGG TCAGGAGTGT
    CAACCGCAGG GTTTGGGCTA CACAGAGGAC GTGACTGAGC ATGAGCAGAT GACCGCAGTG
    CTTTGTACCA GCCTGCAGGG TGGCGATAGT CCATCATCTG TCCTTCCTGG CCTTCGCACA
    CGGACCCGAG CCACTCGAGG TCATACAGCA CTTTTGTCCA CTGCAAATGA AGACACTTCT
    AATTGCACAA ATGACACAGC AGATGAGATC ATGGAGCGGA TTGTGAAATC AGCCACTCAG
    GGCCCCAGTC AGAGAACACA GCCTCGTGAG AGGAGACGTT CCAGAGCAAA CCGCAAATCC
    TTGAGAAGAA CACTGAAGAA CGGTCTTACC CCGGAGGAGG CACAGGCTCT GGGTTTGGCC
    AGCGGCTCTG AGATGCAGGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46043
    Clone ID Related Accession (Same CDS sequence) XM_009292260.2
    Accession Version XM_009292260.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product FH1/FH2 domain-containing protein 3 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292260.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCTACGT TTGCATGCAG GGTGCAGTTC TTAGATGATA CAGATCCATT TAATAGCACA 
    AATTTTCCGG AGCCGACCAG ACCCCCACAT TTCACATTCA GAGAGGATAT TCCGCTCATC
    AATCAGATCG CCGGAGTTCA CAGACTTCTC AAAGCACCTC ACAAGCTTGA TGACTGTGCT
    TTGCAGTTGT CTCATAATGG CAGTTATCTG GACCTGGAGT CCACCTTGGC TGAGCAGAAA
    GATGAACTTG AAGGCTTTCA GGAGGATGGA GGGAGAGGAA AGAAGCACAG TATTGTACTT
    AGAACACAGC TGTCTGTCAG AGTCCATGCC TGCATAGAAA AGCTGTACAA CTCGACGGGT
    CGTGAGCTAC GAAGAGCACT GTTCTCTCTC AAGCAAATCT TCCAGGATGA TAAGGATCTG
    GTTCATGAGT TTGTGATGGC GGAGGGACTC ACATGTCTTA TTAAAGTGGG AGCTGAAGCT
    GATCAAAACT ACCAAAACTA CATTCTCAGA GCACTTGGAC AGATCATGCT GTATGTAGAT
    GGAATGAATG GGCTTATCGG ACACAATGAA ACCGTACAGT GGCTGTACAC ACTGGTTGGA
    TCAAAGTTCC GGTTGGTTGT GAAAACTGCT ATGAAGCTGC TGCTGGTTTT TGTGGAGTAC
    ACCGAGAGCA ACGCACCTCT CCTCATCCAA GCCGTCAACA GCGTTGACAC CAAACAAGGC
    TCTAAGCCCT GGTCTAATGC CATGGAAATC CTTGACGAGA AAGATGGAAT GGACACTGAG
    CTGCTGGTTT ATGCCATGAC ACTAATCAAC AAGACATTAG CTGGTCTGCC TGACCAGGAC
    TCCTTCTATG ATGTGGTTGA CAGTCTGGAG GAGCAGAACA TGGAGGCTGT TGCCCAAAGA
    CACATTAGTA GAAAGGGCAC AGATCTGGAC TTGGTAGAGC AGTTCAACAT TTATGAGGCA
    ACTCTTCGTC ATGAAGACGG TGATGATGAA GCTCAGTCTC CTCCATCTGG ACATAAATGT
    CGGCGTCGGG CTAGTGTTGG AGCAACACCT GAGAGACGGG GTTTGGAACG ACGGCGTAGC
    CGAAGACATT CACTCCAGAA CGGAAAAGGC CACACTTCTG CTCCAACTTC CCCCTGTCAC
    AGCCATACAC CCAACATCCT GTCCTTTAAC AGCCAGAGGA TAGAAGACAT CAGTGAAAGT
    TTGTCAGATG TTAGTTACCC AAACACACCA TGCAGTTCTT GTGGCAGCAA TGGCAATGCA
    AGCACATATC CCGGCAGGTC ATCTGGGGTG TCCAGTGCCC TCTCCACACC CTACAACCGC
    TTCGGTCCTT CAGGAGGTCC ATCTGCAGAC AGTAAAGCCG ACAGGCCAAC TCTGGGTGGA
    TTGTTGACCT CCTCCTACAG GCAGCACCAA GAATCTCTGA ACGCTGAAAG AGAGCGGAGA
    CGTCTGGAGA GAGAAGAGAG ACTTCAACGG ATTGAAAGAG AGGAGAGAAA CCGCTTCAAT
    CGGGATTATG CAGACAAGAG AGAAGAGGTC AGACATGCCC GTGAAGAAAG ATACAAGCAA
    GTGGAGCGTC TGACAGCAGA GGAAAAAGAA AGGGAACGGC CGAGAACACG TGGTAGAACA
    GAAATCACCC TCAGTCTGAG TTCACGCACT TCCAGCAGCT CCTTGTCTCG TTCCGTCACC
    CCTTTGACTG TAGATGCTCA AGAGGAGACA CGCCCTGTCT CACATACAGC ACAAGGAACT
    GTGACTGAGA AAAGTTGTAC GGTGCAGACA GCAGTTGTGC GTTCTTTATC AGAACCTCCC
    TCTGCATCTC CACCAGTGTC CCCATCGCAA CCAGAGGAGC AACAGAGACC TGTCGACCCA
    TCCGACATCA TTCTATCTCT ATCTCCAACA AACTCTGAAC CAGAAAGAAC AGAGAAAGGG
    GAGAAAAAAG AGGATGCTGA AGCAGAGGAG TGTGAGGAAA CAGTGGTGGG TGAAGTGCAA
    GATGAAAAGA AGGAAGAACC AGAAGGAAAC TTTGAGGATG AGACTAATGT GACGGGTGCA
    GAAGACTGTG AGAGGGAGAA GACGTTGGAT AGAGAAGTAG AGGAGAGTGC CATCCTAAGT
    GAAAAGGAGA AGCAGAATGA AGAACTGAAT GAGAAAGATA ACTGCTCTGC CTCCAGCATC
    TCTTCAGCCA GCAGCACTCT GGAGAGAGAA GAAAGAGAGG AGAAACATGT CAGTGACAGC
    AACTCAGGTC AATGGGCAGA CTGTATAAAT ACAGCAGACA ACAACGATCA GTGCAACAAA
    GTTCTTAACA GCAAACGTTT CATGCTGGAC ATGCTGTATG CCCAAAACAA GGAAGGAGAG
    GAAGATGAGA AAGAAAAGGA GAACGAAAAT GAGAAATGTT TAGGTCAGGA CTCTACTGTG
    ACAAGCCTTG CTAGCCGAAT TTCCACTCTG GAATCACATC GGCAGACATG TGAGGACAAC
    TTGAAGAAAC TGGAAGTGGA GCATCTGGAA AACCAGGGCA GCGTGCGGGC TGTAGCTGAG
    AAATTTGGAG ATTTGGTCAA AGGCATGTTA TCACCTCATG ACTTGGAGAA TTGTGGGAAG
    GAAAAGCAGC AAACACAGGA AAAGCCTCCA CCTGCTCCAT CACCAGCACC CCCAAAAAAG
    GAGTCGGACC ACATATGGGA CCAGCTAATG GCAACGCCAC GGGAGCTTCG TATCAAAGAG
    CTGGATTTCA CAGACTTGGG AGAGGAGGAC GATGTGGATA TTTTGGACGT GGGGAACATG
    ATTGGGTCAG GTGACCTCAC TCCACCTCCT CCACCACCGC CCTGCTTGCC CAACCTTCCT
    CCCCCGCCAC CTTTATTTGG ATGCCCTCTG CCTCCCCCAG TCCCTGGCAT GATGATGCCC
    CCTCCACCAC CCTTTTTGGC GTCAAAACCT CCAGCACAAT TGGGATCTCC TCAGCTTAGT
    CGAGGAGAAC CACCGCTCTT CCAAAAGAAG AAGAAGACCA TCAGGCTATT CTGGAATGAG
    GTGAGACCGA TTGACTGGCA GTACACCAAC CACAAGAGAT GCAAGGAGTC ACTCTGGTCC
    AAACTGGATC CCATCAAACT GGACACAGCC AAATTGGAAA ACCTCTTTGA GACGAAATCT
    AAGGAGCTGC CAGTTACAAA GAAAACAGCA GCAGATGGTA AGCGTCAGGA GATCATCATT
    CTGGATTCCA AAAGAAGTAA TGCTATCAAC ATTGGCCTGA CGGTCCTGCC GCCTCCTCGC
    ACCATCAAAA CAGCCATCCT CAACTTTGAT GAGTACGCCT TGAGCAAAGA GGGCATAGAG
    AAAATCCTCA CCATGATTCC TACGGAGGAG GAGACACAGA AGATTCAGGA GGCCCAGCTG
    GCGAACCCCG ACACTCCGCT GGGCAGCGCA GAGCAGTTCC TCCTCATTTT GTCCTCCATC
    AGTGAGCTGT CCGCCCGCCT GCAACTCTGG GCCTTTAAGA TGGACTATGA TGCTTTAGAG
    AAGGAAGTAG CAGAACCACT ACAGGACCTA AAAGAGGGCA TGGACCAATT AGAAAAGAAT
    AAAACGTTAC GCTCTATCCT TTCGACTCTG CTAGCTATTG GCAACTTTCT CAATGGGACC
    AATGCTAAAG GCTTTGAGTT GAGTTATCTA GAGAAAGTTC CTGAAGTGAA GGACACGGTT
    CACAAACAGT CTCTACTGCA TCATGTCTGC TCGATTGTTG TGGAGAAGTT TCCAGAGAGC
    ACAGATCTTT ACTCTGAGAT TGGAGCCATT ACTCGCTCTG CTAAGGTTGA CTTTGATCAG
    CTTCAGGAAA ACCTGTGTCA AATGGAACGG CGCTGCAAAG CATCCTGGGA CCACCTGAAG
    GTCATCTCCA AACACGAGAT TAAACCAGCC CTTAAACAGC GAATGTCCGA CTTCCTCAAA
    GACTGTGCAG GAAGAATTAT CATCCTTAAA ATTGTCCATC GTCGTATCAT AAACAGGTTT
    CACTCATTCC TGCTATTTCT GGGCCACCCA CCATACGCCA TTAGAGAAGT GAGCGTCCAC
    CGCTTCAGCA AGATTCTGAG TGAGTTTGCT CTTGAGTACC GAACCACCAG AGAGCGAGTG
    CTGCAGCAGA AACAGAAACG AGCCAATCAT CGTGAGAGGA ACAAGACCCG CGGCAAAATG
    ATCACAGAGA GTGGGAAGTT TGCAGGAAGT CCTATGGAGG GTCAGGAGTG TCAACCGCAG
    GGTTTGGGCT ACACAGAGGA CGTGACTGAG CATGAGCAGA TGACCGCAGT GCTTTGTACC
    AGCCTGCAGG GTGGCGATAG TCCATCATCT GTCCTTCCTG GCCTTCGCAC ACGGACCCGA
    GCCACTCGAG GTCATACAGC ACTTTTGTCC ACTGCAAATG AAGACACTTC TAATTGCACA
    AATGACACAG CAGATGAGAT CATGGAGCGG ATTGTGAAAT CAGCCACTCA GGGCCCCAGT
    CAGAGAACAC AGCCTCGTGA GAGGAGACGT TCCAGAGCAA ACCGCAAATC CTTGAGAAGA
    ACACTGAAGA ACGGTCTTAC CCCGGAGGAG GCACAGGCTC TGGGTTTGGC CAGCGGCTCT
    GAGATGCAGG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290535.1
    CDS394..4968
    Translation

    Target ORF information:

    RefSeq Version XM_009292260.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio formin homology 2 domain containing 3b (fhod3b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292260.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCTACGT TTGCATGCAG GGTGCAGTTC TTAGATGATA CAGATCCATT TAATAGCACA 
    AATTTTCCGG AGCCGACCAG ACCCCCACAT TTCACATTCA GAGAGGATAT TCCGCTCATC
    AATCAGATCG CCGGAGTTCA CAGACTTCTC AAAGCACCTC ACAAGCTTGA TGACTGTGCT
    TTGCAGTTGT CTCATAATGG CAGTTATCTG GACCTGGAGT CCACCTTGGC TGAGCAGAAA
    GATGAACTTG AAGGCTTTCA GGAGGATGGA GGGAGAGGAA AGAAGCACAG TATTGTACTT
    AGAACACAGC TGTCTGTCAG AGTCCATGCC TGCATAGAAA AGCTGTACAA CTCGACGGGT
    CGTGAGCTAC GAAGAGCACT GTTCTCTCTC AAGCAAATCT TCCAGGATGA TAAGGATCTG
    GTTCATGAGT TTGTGATGGC GGAGGGACTC ACATGTCTTA TTAAAGTGGG AGCTGAAGCT
    GATCAAAACT ACCAAAACTA CATTCTCAGA GCACTTGGAC AGATCATGCT GTATGTAGAT
    GGAATGAATG GGCTTATCGG ACACAATGAA ACCGTACAGT GGCTGTACAC ACTGGTTGGA
    TCAAAGTTCC GGTTGGTTGT GAAAACTGCT ATGAAGCTGC TGCTGGTTTT TGTGGAGTAC
    ACCGAGAGCA ACGCACCTCT CCTCATCCAA GCCGTCAACA GCGTTGACAC CAAACAAGGC
    TCTAAGCCCT GGTCTAATGC CATGGAAATC CTTGACGAGA AAGATGGAAT GGACACTGAG
    CTGCTGGTTT ATGCCATGAC ACTAATCAAC AAGACATTAG CTGGTCTGCC TGACCAGGAC
    TCCTTCTATG ATGTGGTTGA CAGTCTGGAG GAGCAGAACA TGGAGGCTGT TGCCCAAAGA
    CACATTAGTA GAAAGGGCAC AGATCTGGAC TTGGTAGAGC AGTTCAACAT TTATGAGGCA
    ACTCTTCGTC ATGAAGACGG TGATGATGAA GCTCAGTCTC CTCCATCTGG ACATAAATGT
    CGGCGTCGGG CTAGTGTTGG AGCAACACCT GAGAGACGGG GTTTGGAACG ACGGCGTAGC
    CGAAGACATT CACTCCAGAA CGGAAAAGGC CACACTTCTG CTCCAACTTC CCCCTGTCAC
    AGCCATACAC CCAACATCCT GTCCTTTAAC AGCCAGAGGA TAGAAGACAT CAGTGAAAGT
    TTGTCAGATG TTAGTTACCC AAACACACCA TGCAGTTCTT GTGGCAGCAA TGGCAATGCA
    AGCACATATC CCGGCAGGTC ATCTGGGGTG TCCAGTGCCC TCTCCACACC CTACAACCGC
    TTCGGTCCTT CAGGAGGTCC ATCTGCAGAC AGTAAAGCCG ACAGGCCAAC TCTGGGTGGA
    TTGTTGACCT CCTCCTACAG GCAGCACCAA GAATCTCTGA ACGCTGAAAG AGAGCGGAGA
    CGTCTGGAGA GAGAAGAGAG ACTTCAACGG ATTGAAAGAG AGGAGAGAAA CCGCTTCAAT
    CGGGATTATG CAGACAAGAG AGAAGAGGTC AGACATGCCC GTGAAGAAAG ATACAAGCAA
    GTGGAGCGTC TGACAGCAGA GGAAAAAGAA AGGGAACGGC CGAGAACACG TGGTAGAACA
    GAAATCACCC TCAGTCTGAG TTCACGCACT TCCAGCAGCT CCTTGTCTCG TTCCGTCACC
    CCTTTGACTG TAGATGCTCA AGAGGAGACA CGCCCTGTCT CACATACAGC ACAAGGAACT
    GTGACTGAGA AAAGTTGTAC GGTGCAGACA GCAGTTGTGC GTTCTTTATC AGAACCTCCC
    TCTGCATCTC CACCAGTGTC CCCATCGCAA CCAGAGGAGC AACAGAGACC TGTCGACCCA
    TCCGACATCA TTCTATCTCT ATCTCCAACA AACTCTGAAC CAGAAAGAAC AGAGAAAGGG
    GAGAAAAAAG AGGATGCTGA AGCAGAGGAG TGTGAGGAAA CAGTGGTGGG TGAAGTGCAA
    GATGAAAAGA AGGAAGAACC AGAAGGAAAC TTTGAGGATG AGACTAATGT GACGGGTGCA
    GAAGACTGTG AGAGGGAGAA GACGTTGGAT AGAGAAGTAG AGGAGAGTGC CATCCTAAGT
    GAAAAGGAGA AGCAGAATGA AGAACTGAAT GAGAAAGATA ACTGCTCTGC CTCCAGCATC
    TCTTCAGCCA GCAGCACTCT GGAGAGAGAA GAAAGAGAGG AGAAACATGT CAGTGACAGC
    AACTCAGGTC AATGGGCAGA CTGTATAAAT ACAGCAGACA ACAACGATCA GTGCAACAAA
    GTTCTTAACA GCAAACGTTT CATGCTGGAC ATGCTGTATG CCCAAAACAA GGAAGGAGAG
    GAAGATGAGA AAGAAAAGGA GAACGAAAAT GAGAAATGTT TAGGTCAGGA CTCTACTGTG
    ACAAGCCTTG CTAGCCGAAT TTCCACTCTG GAATCACATC GGCAGACATG TGAGGACAAC
    TTGAAGAAAC TGGAAGTGGA GCATCTGGAA AACCAGGGCA GCGTGCGGGC TGTAGCTGAG
    AAATTTGGAG ATTTGGTCAA AGGCATGTTA TCACCTCATG ACTTGGAGAA TTGTGGGAAG
    GAAAAGCAGC AAACACAGGA AAAGCCTCCA CCTGCTCCAT CACCAGCACC CCCAAAAAAG
    GAGTCGGACC ACATATGGGA CCAGCTAATG GCAACGCCAC GGGAGCTTCG TATCAAAGAG
    CTGGATTTCA CAGACTTGGG AGAGGAGGAC GATGTGGATA TTTTGGACGT GGGGAACATG
    ATTGGGTCAG GTGACCTCAC TCCACCTCCT CCACCACCGC CCTGCTTGCC CAACCTTCCT
    CCCCCGCCAC CTTTATTTGG ATGCCCTCTG CCTCCCCCAG TCCCTGGCAT GATGATGCCC
    CCTCCACCAC CCTTTTTGGC GTCAAAACCT CCAGCACAAT TGGGATCTCC TCAGCTTAGT
    CGAGGAGAAC CACCGCTCTT CCAAAAGAAG AAGAAGACCA TCAGGCTATT CTGGAATGAG
    GTGAGACCGA TTGACTGGCA GTACACCAAC CACAAGAGAT GCAAGGAGTC ACTCTGGTCC
    AAACTGGATC CCATCAAACT GGACACAGCC AAATTGGAAA ACCTCTTTGA GACGAAATCT
    AAGGAGCTGC CAGTTACAAA GAAAACAGCA GCAGATGGTA AGCGTCAGGA GATCATCATT
    CTGGATTCCA AAAGAAGTAA TGCTATCAAC ATTGGCCTGA CGGTCCTGCC GCCTCCTCGC
    ACCATCAAAA CAGCCATCCT CAACTTTGAT GAGTACGCCT TGAGCAAAGA GGGCATAGAG
    AAAATCCTCA CCATGATTCC TACGGAGGAG GAGACACAGA AGATTCAGGA GGCCCAGCTG
    GCGAACCCCG ACACTCCGCT GGGCAGCGCA GAGCAGTTCC TCCTCATTTT GTCCTCCATC
    AGTGAGCTGT CCGCCCGCCT GCAACTCTGG GCCTTTAAGA TGGACTATGA TGCTTTAGAG
    AAGGAAGTAG CAGAACCACT ACAGGACCTA AAAGAGGGCA TGGACCAATT AGAAAAGAAT
    AAAACGTTAC GCTCTATCCT TTCGACTCTG CTAGCTATTG GCAACTTTCT CAATGGGACC
    AATGCTAAAG GCTTTGAGTT GAGTTATCTA GAGAAAGTTC CTGAAGTGAA GGACACGGTT
    CACAAACAGT CTCTACTGCA TCATGTCTGC TCGATTGTTG TGGAGAAGTT TCCAGAGAGC
    ACAGATCTTT ACTCTGAGAT TGGAGCCATT ACTCGCTCTG CTAAGGTTGA CTTTGATCAG
    CTTCAGGAAA ACCTGTGTCA AATGGAACGG CGCTGCAAAG CATCCTGGGA CCACCTGAAG
    GTCATCTCCA AACACGAGAT TAAACCAGCC CTTAAACAGC GAATGTCCGA CTTCCTCAAA
    GACTGTGCAG GAAGAATTAT CATCCTTAAA ATTGTCCATC GTCGTATCAT AAACAGGTTT
    CACTCATTCC TGCTATTTCT GGGCCACCCA CCATACGCCA TTAGAGAAGT GAGCGTCCAC
    CGCTTCAGCA AGATTCTGAG TGAGTTTGCT CTTGAGTACC GAACCACCAG AGAGCGAGTG
    CTGCAGCAGA AACAGAAACG AGCCAATCAT CGTGAGAGGA ACAAGACCCG CGGCAAAATG
    ATCACAGAGA GTGGGAAGTT TGCAGGAAGT CCTATGGAGG GTCAGGAGTG TCAACCGCAG
    GGTTTGGGCT ACACAGAGGA CGTGACTGAG CATGAGCAGA TGACCGCAGT GCTTTGTACC
    AGCCTGCAGG GTGGCGATAG TCCATCATCT GTCCTTCCTG GCCTTCGCAC ACGGACCCGA
    GCCACTCGAG GTCATACAGC ACTTTTGTCC ACTGCAAATG AAGACACTTC TAATTGCACA
    AATGACACAG CAGATGAGAT CATGGAGCGG ATTGTGAAAT CAGCCACTCA GGGCCCCAGT
    CAGAGAACAC AGCCTCGTGA GAGGAGACGT TCCAGAGCAA ACCGCAAATC CTTGAGAAGA
    ACACTGAAGA ACGGTCTTAC CCCGGAGGAG GCACAGGCTC TGGGTTTGGC CAGCGGCTCT
    GAGATGCAGG TGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46044
    Clone ID Related Accession (Same CDS sequence) XM_009292261.2
    Accession Version XM_009292261.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4473bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product FH1/FH2 domain-containing protein 3 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292261.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGTCAGC GAGAGATTGC GGGCAGGAAC AATACAAACG AGCTTGAAAA GCTGTACAAC 
    TCGACGGGTC GTGAGCTACG AAGAGCACTG TTCTCTCTCA AGCAAATCTT CCAGGATGAT
    AAGGATCTGG TTCATGAGTT TGTGATGGCG GAGGGACTCA CATGTCTTAT TAAAGTGGGA
    GCTGAAGCTG ATCAAAACTA CCAAAACTAC ATTCTCAGAG CACTTGGACA GATCATGCTG
    TATGTAGATG GAATGAATGG GCTTATCGGA CACAATGAAA CCGTACAGTG GCTGTACACA
    CTGGTTGGAT CAAAGTTCCG GTTGGTTGTG AAAACTGCTA TGAAGCTGCT GCTGGTTTTT
    GTGGAGTACA CCGAGAGCAA CGCACCTCTC CTCATCCAAG CCGTCAACAG CGTTGACACC
    AAACAAGGCT CTAAGCCCTG GTCTAATGCC ATGGAAATCC TTGACGAGAA AGATGGAATG
    GACACTGAGC TGCTGGTTTA TGCCATGACA CTAATCAACA AGACATTAGC TGGTCTGCCT
    GACCAGGACT CCTTCTATGA TGTGGTTGAC AGTCTGGAGG AGCAGAACAT GGAGGCTGTT
    GCCCAAAGAC ACATTAGTAG AAAGGGCACA GATCTGGACT TGGTAGAGCA GTTCAACATT
    TATGAGGCAA CTCTTCGTCA TGAAGACGGT GATGATGAAG CTCAGTCTCC TCCATCTGGA
    CATAAATGTC GGCGTCGGGC TAGTGTTGGA GCAACACCTG AGAGACGGGG TTTGGAACGA
    CGGCGTAGCC GAAGACATTC ACTCCAGAAC GGAAAAGGCC ACACTTCTGC TCCAACTTCC
    CCCTGTCACA GCCATACACC CAACATCCTG TCCTTTAACA GCCAGAGGAT AGAAGACATC
    AGTGAAAGAG AGGATGAGGA AGAGGAGGAG GAAAAGGATG AAGAGTTTCA GAAAGCAGCA
    CTTTCTAGAC AGGCATCTGG AAGCTGTGTG GCATCAACAG TAGAGGACGC TTCTAGCTTG
    TGTTTGCCTT TAAGGTGCAT GGTCAACTTC ATTCCAAATG CTGCTGACAC AGAAAATGCA
    GAAAAACCTC GGAACAGTTT GTCAGATGTT AGTTACCCAA ACACACCATG CAGTTCTTGT
    GGCAGCAATG GCAATGCAAG CACATATCCC GGCAGGTCAT CTGGGGTGTC CAGTGCCCTC
    TCCACACCCT ACAACCGCTT CGGTCCTTCA GGAGGTCCAT CTGCAGACAG TAAAGCCGAC
    AGGCCAACTC TGGGTGGATT GTTGACCTCC TCCTACAGGC AGCACCAAGA ATCTCTGAAC
    GCTGAAAGAG AGCGGAGACG TCTGGAGAGA GAAGAGAGAC TTCAACGGAT TGAAAGAGAG
    GAGAGAAACC GCTTCAATCG GGATTATGCA GACAAGAGAG AAGAGGTCAG ACATGCCCGT
    GAAGAAAGAT ACAAGCAAGT GGAGCGTCTG ACAGCAGAGG AAAAAGAAAG GGAACGGCCG
    AGAACACGTG GTAGAACAGA AATCACCCTC AGTCTGAGTT CACGCACTTC CAGCAGCTCC
    TTGTCTCGTT CCGTCACCCC TTTGACTGTA GATGCTCAAG AGGAGACACG CCCTGTCTCA
    CATACAGCAC AAGGAACTGT GACTGAGAAA AGTTGTACGG TGCAGACAGC AGTTGTGCGT
    TCTTTATCAG AACCTCCCTC TGCATCTCCA CCAGTGTCCC CATCGCAACC AGAGGAGCAA
    CAGAGACCTG TCGACCCATC CGACATCATT CTATCTCTAT CTCCAACAAA CTCTGAACCA
    GAAAGAACAG AGAAAGGGGA GAAAAAAGAG GATGCTGAAG CAGAGGAGTG TGAGGAAACA
    GTGGTGGGTG AAGTGCAAGA TGAAAAGAAG GAAGAACCAG AAGGAAACTT TGAGGATGAG
    ACTAATGTGA CGGGTGCAGA AGACTGTGAG AGGGAGAAGA CGTTGGATAG AGAAGTAGAG
    GAGAGTGCCA TCCTAAGTGA AAAGGAGAAG CAGAATGAAG AACTGAATGA GAAAGATAAC
    TGCTCTGCCT CCAGCATCTC TTCAGCCAGC AGCACTCTGG AGAGAGAAGA AAGAGAGGAG
    AAACATGTCA GTGACAGCAA CTCAGGTCAA TGGGCAGACT GTATAAATAC AGCAGACAAC
    AACGATCAGT GCAACAAAGT TCTTAACAGC AAACGTTTCA TGCTGGACAT GCTGTATGCC
    CAAAACAAGG AAGGAGAGGA AGATGAGAAA GAAAAGGAGA ACGAAAATGA GAAATGTTTA
    GGTCAGGACT CTACTGTGAC AAGCCTTGCT AGCCGAATTT CCACTCTGGA ATCACATCGG
    CAGACATGTG AGGACAACTT GAAGAAACTG GAAGTGGAGC ATCTGGAAAA CCAGGGCAGC
    GTGCGGGCTG TAGCTGAGAA ATTTGGAGAT TTGGTCAAAG GCATGTTATC ACCTCATGAC
    TTGGAGAATT GTGGGAAGGA AAAGCAGCAA ACACAGGAAA AGCCTCCACC TGCTCCATCA
    CCAGCACCCC CAAAAAAGGA GTCGGACCAC ATATGGGACC AGCTAATGGC AACGCCACGG
    GAGCTTCGTA TCAAAGAGCT GGATTTCACA GACTTGGGAG AGGAGGACGA TGTGGATATT
    TTGGACGTGG GGAACATGAT TGGGTCAGGT GACCTCACTC CACCTCCTCC ACCACCGCCC
    TGCTTGCCCA ACCTTCCTCC CCCGCCACCT TTATTTGGAT GCCCTCTGCC TCCCCCAGTC
    CCTGGCATGA TGATGCCCCC TCCACCACCC TTTTTGGCGT CAAAACCTCC AGCACAATTG
    GGATCTCCTC AGCTTAGTCG AGGAGAACCA CCGCTCTTCC AAAAGAAGAA GAAGACCATC
    AGGCTATTCT GGAATGAGGT GAGACCGATT GACTGGCAGT ACACCAACCA CAAGAGATGC
    AAGGAGTCAC TCTGGTCCAA ACTGGATCCC ATCAAACTGG ACACAGCCAA ATTGGAAAAC
    CTCTTTGAGA CGAAATCTAA GGAGCTGCCA GTTACAAAGA AAACAGCAGC AGATGGTAAG
    CGTCAGGAGA TCATCATTCT GGATTCCAAA AGAAGTAATG CTATCAACAT TGGCCTGACG
    GTCCTGCCGC CTCCTCGCAC CATCAAAACA GCCATCCTCA ACTTTGATGA GTACGCCTTG
    AGCAAAGAGG GCATAGAGAA AATCCTCACC ATGATTCCTA CGGAGGAGGA GACACAGAAG
    ATTCAGGAGG CCCAGCTGGC GAACCCCGAC ACTCCGCTGG GCAGCGCAGA GCAGTTCCTC
    CTCATTTTGT CCTCCATCAG TGAGCTGTCC GCCCGCCTGC AACTCTGGGC CTTTAAGATG
    GACTATGATG CTTTAGAGAA GGAAGTAGCA GAACCACTAC AGGACCTAAA AGAGGGCATG
    GACCAATTAG AAAAGAATAA AACGTTACGC TCTATCCTTT CGACTCTGCT AGCTATTGGC
    AACTTTCTCA ATGGGACCAA TGCTAAAGGC TTTGAGTTGA GTTATCTAGA GAAAGTTCCT
    GAAGTGAAGG ACACGGTTCA CAAACAGTCT CTACTGCATC ATGTCTGCTC GATTGTTGTG
    GAGAAGTTTC CAGAGAGCAC AGATCTTTAC TCTGAGATTG GAGCCATTAC TCGCTCTGCT
    AAGGTTGACT TTGATCAGCT TCAGGAAAAC CTGTGTCAAA TGGAACGGCG CTGCAAAGCA
    TCCTGGGACC ACCTGAAGGT CATCTCCAAA CACGAGATTA AACCAGCCCT TAAACAGCGA
    ATGTCCGACT TCCTCAAAGA CTGTGCAGGA AGAATTATCA TCCTTAAAAT TGTCCATCGT
    CGTATCATAA ACAGGTTTCA CTCATTCCTG CTATTTCTGG GCCACCCACC ATACGCCATT
    AGAGAAGTGA GCGTCCACCG CTTCAGCAAG ATTCTGAGTG AGTTTGCTCT TGAGTACCGA
    ACCACCAGAG AGCGAGTGCT GCAGCAGAAA CAGAAACGAG CCAATCATCG TGAGAGGAAC
    AAGACCCGCG GCAAAATGAT CACAGAGAGT GGGAAGTTTG CAGGAAGTCC TATGGAGGGT
    CAGGAGTGTC AACCGCAGGG TTTGGGCTAC ACAGAGGACG TGACTGAGCA TGAGCAGATG
    ACCGCAGTGC TTTGTACCAG CCTGCAGGGT GGCGATAGTC CATCATCTGT CCTTCCTGGC
    CTTCGCACAC GGACCCGAGC CACTCGAGGT CATACAGCAC TTTTGTCCAC TGCAAATGAA
    GACACTTCTA ATTGCACAAA TGACACAGCA GATGAGATCA TGGAGCGGAT TGTGAAATCA
    GCCACTCAGG GCCCCAGTCA GAGAACACAG CCTCGTGAGA GGAGACGTTC CAGAGCAAAC
    CGCAAATCCT TGAGAAGAAC ACTGAAGAAC GGTCTTACCC CGGAGGAGGC ACAGGCTCTG
    GGTTTGGCCA GCGGCTCTGA GATGCAGGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290536.1
    CDS268..4740
    Translation

    Target ORF information:

    RefSeq Version XM_009292261.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio formin homology 2 domain containing 3b (fhod3b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009292261.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGTCAGC GAGAGATTGC GGGCAGGAAC AATACAAACG AGCTTGAAAA GCTGTACAAC 
    TCGACGGGTC GTGAGCTACG AAGAGCACTG TTCTCTCTCA AGCAAATCTT CCAGGATGAT
    AAGGATCTGG TTCATGAGTT TGTGATGGCG GAGGGACTCA CATGTCTTAT TAAAGTGGGA
    GCTGAAGCTG ATCAAAACTA CCAAAACTAC ATTCTCAGAG CACTTGGACA GATCATGCTG
    TATGTAGATG GAATGAATGG GCTTATCGGA CACAATGAAA CCGTACAGTG GCTGTACACA
    CTGGTTGGAT CAAAGTTCCG GTTGGTTGTG AAAACTGCTA TGAAGCTGCT GCTGGTTTTT
    GTGGAGTACA CCGAGAGCAA CGCACCTCTC CTCATCCAAG CCGTCAACAG CGTTGACACC
    AAACAAGGCT CTAAGCCCTG GTCTAATGCC ATGGAAATCC TTGACGAGAA AGATGGAATG
    GACACTGAGC TGCTGGTTTA TGCCATGACA CTAATCAACA AGACATTAGC TGGTCTGCCT
    GACCAGGACT CCTTCTATGA TGTGGTTGAC AGTCTGGAGG AGCAGAACAT GGAGGCTGTT
    GCCCAAAGAC ACATTAGTAG AAAGGGCACA GATCTGGACT TGGTAGAGCA GTTCAACATT
    TATGAGGCAA CTCTTCGTCA TGAAGACGGT GATGATGAAG CTCAGTCTCC TCCATCTGGA
    CATAAATGTC GGCGTCGGGC TAGTGTTGGA GCAACACCTG AGAGACGGGG TTTGGAACGA
    CGGCGTAGCC GAAGACATTC ACTCCAGAAC GGAAAAGGCC ACACTTCTGC TCCAACTTCC
    CCCTGTCACA GCCATACACC CAACATCCTG TCCTTTAACA GCCAGAGGAT AGAAGACATC
    AGTGAAAGAG AGGATGAGGA AGAGGAGGAG GAAAAGGATG AAGAGTTTCA GAAAGCAGCA
    CTTTCTAGAC AGGCATCTGG AAGCTGTGTG GCATCAACAG TAGAGGACGC TTCTAGCTTG
    TGTTTGCCTT TAAGGTGCAT GGTCAACTTC ATTCCAAATG CTGCTGACAC AGAAAATGCA
    GAAAAACCTC GGAACAGTTT GTCAGATGTT AGTTACCCAA ACACACCATG CAGTTCTTGT
    GGCAGCAATG GCAATGCAAG CACATATCCC GGCAGGTCAT CTGGGGTGTC CAGTGCCCTC
    TCCACACCCT ACAACCGCTT CGGTCCTTCA GGAGGTCCAT CTGCAGACAG TAAAGCCGAC
    AGGCCAACTC TGGGTGGATT GTTGACCTCC TCCTACAGGC AGCACCAAGA ATCTCTGAAC
    GCTGAAAGAG AGCGGAGACG TCTGGAGAGA GAAGAGAGAC TTCAACGGAT TGAAAGAGAG
    GAGAGAAACC GCTTCAATCG GGATTATGCA GACAAGAGAG AAGAGGTCAG ACATGCCCGT
    GAAGAAAGAT ACAAGCAAGT GGAGCGTCTG ACAGCAGAGG AAAAAGAAAG GGAACGGCCG
    AGAACACGTG GTAGAACAGA AATCACCCTC AGTCTGAGTT CACGCACTTC CAGCAGCTCC
    TTGTCTCGTT CCGTCACCCC TTTGACTGTA GATGCTCAAG AGGAGACACG CCCTGTCTCA
    CATACAGCAC AAGGAACTGT GACTGAGAAA AGTTGTACGG TGCAGACAGC AGTTGTGCGT
    TCTTTATCAG AACCTCCCTC TGCATCTCCA CCAGTGTCCC CATCGCAACC AGAGGAGCAA
    CAGAGACCTG TCGACCCATC CGACATCATT CTATCTCTAT CTCCAACAAA CTCTGAACCA
    GAAAGAACAG AGAAAGGGGA GAAAAAAGAG GATGCTGAAG CAGAGGAGTG TGAGGAAACA
    GTGGTGGGTG AAGTGCAAGA TGAAAAGAAG GAAGAACCAG AAGGAAACTT TGAGGATGAG
    ACTAATGTGA CGGGTGCAGA AGACTGTGAG AGGGAGAAGA CGTTGGATAG AGAAGTAGAG
    GAGAGTGCCA TCCTAAGTGA AAAGGAGAAG CAGAATGAAG AACTGAATGA GAAAGATAAC
    TGCTCTGCCT CCAGCATCTC TTCAGCCAGC AGCACTCTGG AGAGAGAAGA AAGAGAGGAG
    AAACATGTCA GTGACAGCAA CTCAGGTCAA TGGGCAGACT GTATAAATAC AGCAGACAAC
    AACGATCAGT GCAACAAAGT TCTTAACAGC AAACGTTTCA TGCTGGACAT GCTGTATGCC
    CAAAACAAGG AAGGAGAGGA AGATGAGAAA GAAAAGGAGA ACGAAAATGA GAAATGTTTA
    GGTCAGGACT CTACTGTGAC AAGCCTTGCT AGCCGAATTT CCACTCTGGA ATCACATCGG
    CAGACATGTG AGGACAACTT GAAGAAACTG GAAGTGGAGC ATCTGGAAAA CCAGGGCAGC
    GTGCGGGCTG TAGCTGAGAA ATTTGGAGAT TTGGTCAAAG GCATGTTATC ACCTCATGAC
    TTGGAGAATT GTGGGAAGGA AAAGCAGCAA ACACAGGAAA AGCCTCCACC TGCTCCATCA
    CCAGCACCCC CAAAAAAGGA GTCGGACCAC ATATGGGACC AGCTAATGGC AACGCCACGG
    GAGCTTCGTA TCAAAGAGCT GGATTTCACA GACTTGGGAG AGGAGGACGA TGTGGATATT
    TTGGACGTGG GGAACATGAT TGGGTCAGGT GACCTCACTC CACCTCCTCC ACCACCGCCC
    TGCTTGCCCA ACCTTCCTCC CCCGCCACCT TTATTTGGAT GCCCTCTGCC TCCCCCAGTC
    CCTGGCATGA TGATGCCCCC TCCACCACCC TTTTTGGCGT CAAAACCTCC AGCACAATTG
    GGATCTCCTC AGCTTAGTCG AGGAGAACCA CCGCTCTTCC AAAAGAAGAA GAAGACCATC
    AGGCTATTCT GGAATGAGGT GAGACCGATT GACTGGCAGT ACACCAACCA CAAGAGATGC
    AAGGAGTCAC TCTGGTCCAA ACTGGATCCC ATCAAACTGG ACACAGCCAA ATTGGAAAAC
    CTCTTTGAGA CGAAATCTAA GGAGCTGCCA GTTACAAAGA AAACAGCAGC AGATGGTAAG
    CGTCAGGAGA TCATCATTCT GGATTCCAAA AGAAGTAATG CTATCAACAT TGGCCTGACG
    GTCCTGCCGC CTCCTCGCAC CATCAAAACA GCCATCCTCA ACTTTGATGA GTACGCCTTG
    AGCAAAGAGG GCATAGAGAA AATCCTCACC ATGATTCCTA CGGAGGAGGA GACACAGAAG
    ATTCAGGAGG CCCAGCTGGC GAACCCCGAC ACTCCGCTGG GCAGCGCAGA GCAGTTCCTC
    CTCATTTTGT CCTCCATCAG TGAGCTGTCC GCCCGCCTGC AACTCTGGGC CTTTAAGATG
    GACTATGATG CTTTAGAGAA GGAAGTAGCA GAACCACTAC AGGACCTAAA AGAGGGCATG
    GACCAATTAG AAAAGAATAA AACGTTACGC TCTATCCTTT CGACTCTGCT AGCTATTGGC
    AACTTTCTCA ATGGGACCAA TGCTAAAGGC TTTGAGTTGA GTTATCTAGA GAAAGTTCCT
    GAAGTGAAGG ACACGGTTCA CAAACAGTCT CTACTGCATC ATGTCTGCTC GATTGTTGTG
    GAGAAGTTTC CAGAGAGCAC AGATCTTTAC TCTGAGATTG GAGCCATTAC TCGCTCTGCT
    AAGGTTGACT TTGATCAGCT TCAGGAAAAC CTGTGTCAAA TGGAACGGCG CTGCAAAGCA
    TCCTGGGACC ACCTGAAGGT CATCTCCAAA CACGAGATTA AACCAGCCCT TAAACAGCGA
    ATGTCCGACT TCCTCAAAGA CTGTGCAGGA AGAATTATCA TCCTTAAAAT TGTCCATCGT
    CGTATCATAA ACAGGTTTCA CTCATTCCTG CTATTTCTGG GCCACCCACC ATACGCCATT
    AGAGAAGTGA GCGTCCACCG CTTCAGCAAG ATTCTGAGTG AGTTTGCTCT TGAGTACCGA
    ACCACCAGAG AGCGAGTGCT GCAGCAGAAA CAGAAACGAG CCAATCATCG TGAGAGGAAC
    AAGACCCGCG GCAAAATGAT CACAGAGAGT GGGAAGTTTG CAGGAAGTCC TATGGAGGGT
    CAGGAGTGTC AACCGCAGGG TTTGGGCTAC ACAGAGGACG TGACTGAGCA TGAGCAGATG
    ACCGCAGTGC TTTGTACCAG CCTGCAGGGT GGCGATAGTC CATCATCTGT CCTTCCTGGC
    CTTCGCACAC GGACCCGAGC CACTCGAGGT CATACAGCAC TTTTGTCCAC TGCAAATGAA
    GACACTTCTA ATTGCACAAA TGACACAGCA GATGAGATCA TGGAGCGGAT TGTGAAATCA
    GCCACTCAGG GCCCCAGTCA GAGAACACAG CCTCGTGAGA GGAGACGTTC CAGAGCAAAC
    CGCAAATCCT TGAGAAGAAC ACTGAAGAAC GGTCTTACCC CGGAGGAGGC ACAGGCTCTG
    GGTTTGGCCA GCGGCTCTGA GATGCAGGTG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.