asap2a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	asap2a
Entrez Gene ID	570669
Full Name	ArfGAP with SH3 domain, ankyrin repeat and PH domain 2a
Synonyms	ddef2a,si:dkey-27p7.2
General protein information	Preferred Names ArfGAP with SH3 domain, ankyrin repeat and PH domain 2a Names arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 development and differentiation enhancing factor 2a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021467217.1	XP_021322892.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X1
XM_021467218.1	XP_021322893.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X2
XM_005158734.4	XP_005158791.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X3
XM_005158734.3	XP_005158791.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X1
XM_021467219.1	XP_021322894.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X4
XM_017351668.1	XP_017207157.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X2
NM_001045207.1	NP_001038672.1	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ASAP2	XP_515290.3
Canis lupus familiaris (dog)	ASAP2	XP_005630157.1
Mus musculus (house mouse)	Asap2	NP_001128664.1
Rattus norvegicus (Norway rat)	Asap2	XP_006240060.1
Gallus gallus (chicken)	ASAP2	XP_001231632.3
Xenopus tropicalis (tropical clawed frog)	asap2	XP_002935078.2
Homo sapiens (human)	ASAP2	NP_003878.1
	ASAP2	XP_001088227.2
Bos taurus (cattle)	ASAP2	XP_002691576.2
Danio rerio (zebrafish)	asap2a	NP_001038672.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE, Brownstein MJ, Usdin TB, Toshiyuki S, Carninci P, Prange C, Raha SS, Loquellano NA, Peters GJ, Abramson RD, Mullahy SJ, Bosak SA, McEwan PJ, McKernan KJ, Malek JA, Gunaratne PH, Richards S, Worley KC, Hale S, Garcia AM, Gay LJ, Hulyk SW, Villalon DK, Muzny DM, Sodergren EJ, Lu X, Gibbs RA, Fahey J, Helton E, Ketteman M, Madan A, Rodrigues S, Sanchez A, Whiting M, Madan A, Young AC, Shevchenko Y, Bouffard GG, Blakesley RW, Touchman JW, Green ED, Dickson MC, Rodriguez AC, Grimwood J, Schmutz J, Myers RM, Butterfield YS, Krzywinski MI, Skalska U, Smailus DE, Schnerch A, Schein JE, Jones SJ, Marra MA,
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following asap2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the asap2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa35299	NM_001045207.1 Latest version!	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2a (asap2a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa22692	XM_005158734.4 Latest version!	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2a (asap2a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa149579	XM_021467219.1 Latest version!	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2a (asap2a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa55607	XM_017351668.1 Latest version!	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2a (asap2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa149578	XM_021467218.1 Latest version!	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2a (asap2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa46006	XM_021467217.1 Latest version!	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2a (asap2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
View Old Accession Versions >>
ODa22692	XM_005158734.3	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2a (asap2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

asap2a ( NM_001045207.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001038672.1 Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2a (asap2a), mRNA.

Price & Availability↑

CloneID	ODa35299
Clone ID Related Accession (Same CDS sequence)	NM_001045207.1
Accession Version	NM_001045207.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2976bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR381585.11. On Sep 1, 2006 this sequence version replaced XM_694174.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCCTGACC AGATAACAGT GGCGGAGTTT GTCACGGAGA CAAATGAAGA TTATAAATCG 
CCCACCGCCT CAAACTTCAC CACCAGAATG ACTCACTGCA GGAACACAGT ATCCGCACTG 
GAGGAGGCCC TGGATGTGGA CCGCAGTGTC CTTTACAAGA TGAAGAAGTC AGTTAAGGCT 
ATTTACGCCT CGGGTCTGGC TCATGTGGAG AATGAGGAGC AGTACACTCA AGCTCTGGAG 
AAGTTCGGAG AGAACTGTGT GTACAGAGAT GACCCGGACC TGGGATCAGC CTTCCTGAAG 
TTCTCCGTCT TCACCAAGGA GCTCACGGCA CTCTTCAAGA ACCTGTTTCA GAACATGAAT 
AATATCATTA CCTTCCCATT GGACAGTCTG CTGAAGGGAG ATCTGAAAGG GGTTAAAGGG 
GATCTCAAGA AGCCCTTCGA TAAAGCCTGG AAAGACTACG AGACTAAAGT CTCTAAAATA 
GAGAAGGAGA AAAAAGAGCA CGCCCGGCAG CACGGAATGA TCCGGACGGA GATCAGCGGA 
GCAGAGATAG CAGAAGAGAT GGAAAAAGAG CGGCGTTTCT TCCAGCTTCA GATGTGTGAG 
TACCTCCTCA AAGTCAATGA AATCAAGATC AAAAAAGGTG TCGACCTGCT CCAGAATCTC 
ATCAAATACT TCCACGCACA GTGCAACTTC TTTCAGGATG GTCTCAAAGC GGTGGACAAC 
CTCAAACCCT CAATAGAAAA ACTGGCCACA GACTTGCACT CGATCAAACA GGTACAGGAT 
GAAGAACGCA GACAGCTAAC CCAGTTACGG GATGTGCTAA AAACTGCTCT GCAAGTGGAG 
CAGAAGGAGG ACTCTCAGGT TAGACAGAGC GCCACCTACA GTCTGCACCA GCCGCAGGGC 
AACAAAGAGC ATGGGACTGA GCGCAGCGGC AACCTTTACA AGAAGAGTGA CGGGCTGCGG 
AAAGTGTGGC AGAAGAGAAA GTGCACAGTA AAGAATGGAT ATTTGACCAT CTCACATGGG 
ACGGCAAACA GACCTCCCGC CAAACTCAAT CTTCTCACCT GTCAGGTGAA GCACAACCCA 
GAGGAGAAGA AAAGTTTTGA CCTCATCTCA CATGACAGAA CATATCATTT CCAGGCAGAA 
GATGAGCCAG AGTGTCAAAT ATGGATCTCA GTGCTGCAGA ACAGTAAAGA AGAGGCGCTC 
AACAACGCCT TCAAGGGCGA CCAGCATGTT GGTGAAAATA ACATTGTGCA GGAGCTCACC 
AAGGCCATCC TGGGAGAGGT GAAGCGGATG GCGGGGAACG ATGTCTGCTG CGACTGCGGT 
GCTCCCGGCC CCACATGGCT CTCCACCAAC CTGGGCATCC TGACCTGCAT CGAGTGTTCG 
GGGATCCACA GAGAGCTGGG CGTCCATTAC TCCCGAATCC AGTCCCTCAC ACTCGACGTC 
CTCAGCACCT CCGAGCTCTT GCTGGCCAAG AACGTGGGGA ATGCTGGCTT CAATGAGATC 
ATGGAGGCCT GTCTGACGGC AGAAGATGTG ATCAAACCGA ATCCAGCCAG TGACATGCAG 
GCGAGGAAGG ACTTTATCAT GGCCAAATAC ACAGAGAAAC GCTTCGCTCG TAAGAAGTGT 
CCAGACGCAC TGTCGAAGCT GCACACGCTC TGTGATGCTG TGAAGGCCCG GGACATTTTC 
TCTCTCATCC AGGTCTATGC TGAAGGAGTG GATCTGATGG AGCCCATTCC TCTGGCTAAT 
GGACATGAAC AAGGTGAGAC GGCTCTTCAT CTGGCCGTGA GACTGGTGGA CAGAACTTCC 
CTACACATCA TCGACTTCCT CACCCAAAAC AGTTTAAACC TGGATAAGCA AACGGCTAAA 
GGAAGCACAG CTCTGCATTA CTGCTGCCTG ACGGACAACA GCGAGTGTCT CAAACTGCTG 
CTCAGAGGAA AAGCCTCCAT AGATATCGCT AATGAAGCTG GAGAGACCCC GTTGGACATC 
GCCAGGCGAC TCAAACATCT GCAGTGTGAG GAACTGCTGA ACCAGGCTCT TGCAGGGAAG 
TTCAATGCTC ATGTGCATGT GGAGTATGAG TGGAGACTTC AGCATGAAGA CCTGGACGAG 
AGTGATGAAG ATCTGGATGA GAAGTCGAGT CCTCACCGGC GGGATGAGCG GCCCATCAGC 
TGCTACACAC CGGGCAGTAA CTCCCTTCAG CTGAGTCCAG CCAGCCTGAG CCGAGACGGT 
CGAGACCTGG TTAAAGACAA GCAACGCTTT GTGCCAAACC TGGTCAACAA TGAAACCTAC 
GGGACCATCA TTAACACCAG CTCACCCGTC AGCCTGTCCT CTTCTGCTCC ACCTCTACCA 
CCCCGAAACC TAGTTCAGCC GTCTGCTCTT GCAGGACTGA CTCAAGGATC TCCCGGCTGG 
AAGCCTGGCT CTCTGGATCT GAGCGGCAGA CAGAGATCCT CCTCTGACCC TCCCAACATG 
CATCCTCCTG CGCCTCCCTT ACGGGTCACT TCCACCTCCC TTCTAATGCC CAGCGGTGCT 
GCTCCTCCTC TGGCTAAAGC TACTGGTATG ATGGAGACCA TGAATATGCA ACCCAAACCC 
GGACAGGGGC CTCCTGGACA GAACATCAAC CGGGCTACAA GTGCGGACAA AAACTTCAGC 
AAAAGCACAC TGATGCGCTC CGGATCCATC GAGAGACCAG GTAGATGTCA GAGAAACCAT 
GTTAACTCTA AAGAAGTCCC AGGAGGCCCA CAAAACACCA CTGGTCAAAC TCTGCCTGCG 
ACCCACATGC CCAGGAAAAC GTATTTGAAG CCGAAGCGTG TGAAGGCCAT GTATAACTGT 
GTGGCCGATA ATCCAGACGA GCTGACCTTC TCTGAGGGAG AGCTTATCGT GGTGGATGGA 
GAGGAGGACC AGGAGTGGTG GCTGGGCCAC ATTGAGGGAG AGCCAATGAG AAGAGGAGCG 
TTTCCTGTCA CGTTTGTACA GTTCATTATG GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038672.1
CDS	419..3394
Translation	MPDQITVAEFVTETNEDYKSPTASNFTTRMTHCRNTVSALEEALDVDRSVLYKMKKSVKAIYASGLAHVENEEQYTQALEKFGENCVYRDDPDLGSAFLKFSVFTKELTALFKNLFQNMNNIITFPLDSLLKGDLKGVKGDLKKPFDKAWKDYETKVSKIEKEKKEHARQHGMIRTEISGAEIAEEMEKERRFFQLQMCEYLLKVNEIKIKKGVDLLQNLIKYFHAQCNFFQDGLKAVDNLKPSIEKLATDLHSIKQVQDEERRQLTQLRDVLKTALQVEQKEDSQVRQSATYSLHQPQGNKEHGTERSGNLYKKSDGLRKVWQKRKCTVKNGYLTISHGTANRPPAKLNLLTCQVKHNPEEKKSFDLISHDRTYHFQAEDEPECQIWISVLQNSKEEALNNAFKGDQHVGENNIVQELTKAILGEVKRMAGNDVCCDCGAPGPTWLSTNLGILTCIECSGIHRELGVHYSRIQSLTLDVLSTSELLLAKNVGNAGFNEIMEACLTAEDVIKPNPASDMQARKDFIMAKYTEKRFARKKCPDALSKLHTLCDAVKARDIFSLIQVYAEGVDLMEPIPLANGHEQGETALHLAVRLVDRTSLHIIDFLTQNSLNLDKQTAKGSTALHYCCLTDNSECLKLLLRGKASIDIANEAGETPLDIARRLKHLQCEELLNQALAGKFNAHVHVEYEWRLQHEDLDESDEDLDEKSSPHRRDERPISCYTPGSNSLQLSPASLSRDGRDLVKDKQRFVPNLVNNETYGTIINTSSPVSLSSSAPPLPPRNLVQPSALAGLTQGSPGWKPGSLDLSGRQRSSSDPPNMHPPAPPLRVTSTSLLMPSGAAPPLAKATGMMETMNMQPKPGQGPPGQNINRATSADKNFSKSTLMRSGSIERPGRCQRNHVNSKEVPGGPQNTTGQTLPATHMPRKTYLKPKRVKAMYNCVADNPDELTFSEGELIVVDGEEDQEWWLGHIEGEPMRRGAFPVTFVQFIMD

Target ORF information:

RefSeq Version	NM_001045207.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2a (asap2a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045207.1

ORF Insert Sequence:

ATGCCTGACC AGATAACAGT GGCGGAGTTT GTCACGGAGA CAAATGAAGA TTATAAATCG 
CCCACCGCCT CAAACTTCAC CACCAGAATG ACTCACTGCA GGAACACAGT ATCCGCACTG 
GAGGAGGCCC TGGATGTGGA CCGCAGTGTC CTTTACAAGA TGAAGAAGTC AGTTAAGGCT 
ATTTACGCCT CGGGTCTGGC TCATGTGGAG AATGAGGAGC AGTACACTCA AGCTCTGGAG 
AAGTTCGGAG AGAACTGTGT GTACAGAGAT GACCCGGACC TGGGATCAGC CTTCCTGAAG 
TTCTCCGTCT TCACCAAGGA GCTCACGGCA CTCTTCAAGA ACCTGTTTCA GAACATGAAT 
AATATCATTA CCTTCCCATT GGACAGTCTG CTGAAGGGAG ATCTGAAAGG GGTTAAAGGG 
GATCTCAAGA AGCCCTTCGA TAAAGCCTGG AAAGACTACG AGACTAAAGT CTCTAAAATA 
GAGAAGGAGA AAAAAGAGCA CGCCCGGCAG CACGGAATGA TCCGGACGGA GATCAGCGGA 
GCAGAGATAG CAGAAGAGAT GGAAAAAGAG CGGCGTTTCT TCCAGCTTCA GATGTGTGAG 
TACCTCCTCA AAGTCAATGA AATCAAGATC AAAAAAGGTG TCGACCTGCT CCAGAATCTC 
ATCAAATACT TCCACGCACA GTGCAACTTC TTTCAGGATG GTCTCAAAGC GGTGGACAAC 
CTCAAACCCT CAATAGAAAA ACTGGCCACA GACTTGCACT CGATCAAACA GGTACAGGAT 
GAAGAACGCA GACAGCTAAC CCAGTTACGG GATGTGCTAA AAACTGCTCT GCAAGTGGAG 
CAGAAGGAGG ACTCTCAGGT TAGACAGAGC GCCACCTACA GTCTGCACCA GCCGCAGGGC 
AACAAAGAGC ATGGGACTGA GCGCAGCGGC AACCTTTACA AGAAGAGTGA CGGGCTGCGG 
AAAGTGTGGC AGAAGAGAAA GTGCACAGTA AAGAATGGAT ATTTGACCAT CTCACATGGG 
ACGGCAAACA GACCTCCCGC CAAACTCAAT CTTCTCACCT GTCAGGTGAA GCACAACCCA 
GAGGAGAAGA AAAGTTTTGA CCTCATCTCA CATGACAGAA CATATCATTT CCAGGCAGAA 
GATGAGCCAG AGTGTCAAAT ATGGATCTCA GTGCTGCAGA ACAGTAAAGA AGAGGCGCTC 
AACAACGCCT TCAAGGGCGA CCAGCATGTT GGTGAAAATA ACATTGTGCA GGAGCTCACC 
AAGGCCATCC TGGGAGAGGT GAAGCGGATG GCGGGGAACG ATGTCTGCTG CGACTGCGGT 
GCTCCCGGCC CCACATGGCT CTCCACCAAC CTGGGCATCC TGACCTGCAT CGAGTGTTCG 
GGGATCCACA GAGAGCTGGG CGTCCATTAC TCCCGAATCC AGTCCCTCAC ACTCGACGTC 
CTCAGCACCT CCGAGCTCTT GCTGGCCAAG AACGTGGGGA ATGCTGGCTT CAATGAGATC 
ATGGAGGCCT GTCTGACGGC AGAAGATGTG ATCAAACCGA ATCCAGCCAG TGACATGCAG 
GCGAGGAAGG ACTTTATCAT GGCCAAATAC ACAGAGAAAC GCTTCGCTCG TAAGAAGTGT 
CCAGACGCAC TGTCGAAGCT GCACACGCTC TGTGATGCTG TGAAGGCCCG GGACATTTTC 
TCTCTCATCC AGGTCTATGC TGAAGGAGTG GATCTGATGG AGCCCATTCC TCTGGCTAAT 
GGACATGAAC AAGGTGAGAC GGCTCTTCAT CTGGCCGTGA GACTGGTGGA CAGAACTTCC 
CTACACATCA TCGACTTCCT CACCCAAAAC AGTTTAAACC TGGATAAGCA AACGGCTAAA 
GGAAGCACAG CTCTGCATTA CTGCTGCCTG ACGGACAACA GCGAGTGTCT CAAACTGCTG 
CTCAGAGGAA AAGCCTCCAT AGATATCGCT AATGAAGCTG GAGAGACCCC GTTGGACATC 
GCCAGGCGAC TCAAACATCT GCAGTGTGAG GAACTGCTGA ACCAGGCTCT TGCAGGGAAG 
TTCAATGCTC ATGTGCATGT GGAGTATGAG TGGAGACTTC AGCATGAAGA CCTGGACGAG 
AGTGATGAAG ATCTGGATGA GAAGTCGAGT CCTCACCGGC GGGATGAGCG GCCCATCAGC 
TGCTACACAC CGGGCAGTAA CTCCCTTCAG CTGAGTCCAG CCAGCCTGAG CCGAGACGGT 
CGAGACCTGG TTAAAGACAA GCAACGCTTT GTGCCAAACC TGGTCAACAA TGAAACCTAC 
GGGACCATCA TTAACACCAG CTCACCCGTC AGCCTGTCCT CTTCTGCTCC ACCTCTACCA 
CCCCGAAACC TAGTTCAGCC GTCTGCTCTT GCAGGACTGA CTCAAGGATC TCCCGGCTGG 
AAGCCTGGCT CTCTGGATCT GAGCGGCAGA CAGAGATCCT CCTCTGACCC TCCCAACATG 
CATCCTCCTG CGCCTCCCTT ACGGGTCACT TCCACCTCCC TTCTAATGCC CAGCGGTGCT 
GCTCCTCCTC TGGCTAAAGC TACTGGTATG ATGGAGACCA TGAATATGCA ACCCAAACCC 
GGACAGGGGC CTCCTGGACA GAACATCAAC CGGGCTACAA GTGCGGACAA AAACTTCAGC 
AAAAGCACAC TGATGCGCTC CGGATCCATC GAGAGACCAG GTAGATGTCA GAGAAACCAT 
GTTAACTCTA AAGAAGTCCC AGGAGGCCCA CAAAACACCA CTGGTCAAAC TCTGCCTGCG 
ACCCACATGC CCAGGAAAAC GTATTTGAAG CCGAAGCGTG TGAAGGCCAT GTATAACTGT 
GTGGCCGATA ATCCAGACGA GCTGACCTTC TCTGAGGGAG AGCTTATCGT GGTGGATGGA 
GAGGAGGACC AGGAGTGGTG GCTGGGCCAC ATTGAGGGAG AGCCAATGAG AAGAGGAGCG 
TTTCCTGTCA CGTTTGTACA GTTCATTATG GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap2a ( XM_005158734.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158791.1 Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2a (asap2a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa22692
Clone ID Related Accession (Same CDS sequence)	XM_005158734.4 , XM_005158734.3
Accession Version	XM_005158734.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158734.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGACC AGATAACAGT GGCGGAGTTT GTCACGGAGA CAAATGAAGA TTATAAATCG 
CCCACCGCCT CAAACTTCAC CACCAGAATG ACTCACTGCA GGAACACAGT ATCCGCACTG 
GAGGAGGCCC TGGATGTGGA CCGCAGTGTC CTTTACAAGA TGAAGAAGTC AGTTAAGGCT 
ATTTACGCCT CGGGTCTGGC TCATGTGGAG AATGAGGAGC AGTACACTCA AGCTCTGGAG 
AAGTTCGGAG AGAACTGTGT GTACAGAGAT GACCCGGACC TGGGATCAGC CTTCCTGAAG 
TTCTCCGTCT TCACCAAGGA GCTCACGGCA CTCTTCAAGA ACCTGTTTCA GAACATGAAT 
AATATCATTA CCTTCCCATT GGACAGTCTG CTGAAGGGAG ATCTGAAAGG GGTTAAAGGG 
GATCTCAAGA AGCCCTTCGA TAAAGCCTGG AAAGACTACG AGACTAAAGT CTCTAAAATA 
GAGAAGGAGA AAAAAGAGCA CGCCCGGCAG CACGGAATGA TCCGGACGGA GATCAGCGGA 
GCAGAGATAG CAGAAGAGAT GGAAAAAGAG CGGCGTTTCT TCCAGCTTCA GATGTGTGAG 
TACCTCCTCA AAGTCAATGA AATCAAGATC AAAAAAGGTG TCGACCTGCT CCAGAATCTC 
ATCAAATACT TCCACGCACA GTGCAACTTC TTTCAGGATG GTCTCAAAGC GGTGGACAAC 
CTCAAACCCT CAATAGAAAA ACTGGCCACA GACTTGCACT CGATCAAACA GGTACAGGAT 
GAAGAACGCA GACAGCTAAC CCAGTTACGG GATGTGCTAA AAACTGCTCT GCAAGTGGAG 
CAGAAGGAGT CTAGGAGAGA CTCTCAGGTT AGACAGAGCG CCACCTACAG TCTGCACCAG 
CCGCAGGGCA ACAAAGAGCA TGGGACTGAG CGCAGCGGCA ACCTTTACAA GAAGAGTGAC 
GGGCTGCGGA AAGTGTGGCA GAAGAGAAAG TGCACAGTAA AGAATGGATA TTTGACCATC 
TCACATGGGA CGGCAAACAG ACCTCCCGCC AAACTCAATC TTCTCACCTG TCAGGTGAAG 
CACAACCCAG AGGAGAAGAA AAGTTTTGAC CTCATCTCAC ATGACAGAAC ATATCATTTC 
CAGGCAGAAG ATGAGCCAGA GTGTCAAATA TGGATCTCAG TGCTGCAGAA CAGTAAAGAA 
GAGGCGCTCA ACAACGCCTT CAAGGGCGAC CAGCATGTTG GTGAAAATAA CATTGTGCAG 
GAGCTCACCA AGGCCATCCT GGGAGAGGTG AAGCGGATGG CGGGGAACGA TGTCTGCTGC 
GACTGCGGTG CTCCCGGCCC CACATGGCTC TCCACCAACC TGGGCATCCT GACCTGCATC 
GAGTGTTCGG GGATCCACAG AGAGCTGGGC GTCCATTACT CCCGAATCCA GTCCCTCACA 
CTCGACGTCC TCAGCACCTC CGAGCTCTTG CTGGCCAAGA ACGTGGGGAA TGCTGGCTTC 
AATGAGATCA TGGAGGCCTG TCTGACGGCA GAAGATGTGA TCAAACCGAA TCCAGCCAGT 
GACATGCAGG CGAGGAAGGA CTTTATCATG GCCAAATACA CAGAGAAACG CTTCGCTCGT 
AAGAAGTGTC CAGACGCACT GTCGAAGCTG CACACGCTCT GTGATGCTGT GAAGGCCCGG 
GACATTTTCT CTCTCATCCA GGTCTATGCT GAAGGAGTGG ATCTGATGGA GCCCATTCCT 
CTGGCTAATG GACATGAACA AGGTGAGACG GCTCTTCATC TGGCCGTGAG ACTGGTGGAC 
AGAACTTCCC TACACATCAT CGACTTCCTC ACCCAAAACA GTTTAAACCT GGATAAGCAA 
ACGGCTAAAG GAAGCACAGC TCTGCATTAC TGCTGCCTGA CGGACAACAG CGAGTGTCTC 
AAACTGCTGC TCAGAGGAAA AGCCTCCATA GATATCGCTA ATGAAGCTGG AGAGACCCCG 
TTGGACATCG CCAGGCGACT CAAACATCTG CAGTGTGAGG AACTGCTGAA CCAGGCTCTT 
GCAGGGAAGT TCAATGCTCA TGTGCATGTG GAGTATGAGT GGAGACTTCA GCATGAAGAC 
CTGGACGAGA GTGATGAAGA TCTGGATGAG AAGTCGAGTC CTCACCGGCG GGATGAGCGG 
CCCATCAGCT GCTACACACC GGGCAGTAAC TCCCTTCAGC TGAGTCCAGC CAGCCTGAGC 
CGAGACGGTC GAGACCTGGT TAAAGACAAG CAACGCTTTG TGCCAAACCT GGTCAACAAT 
GAAACCTACG GGACCATCAT TAACACCAGC TCACCCGTCA GCCTGTCCTC TTCTGCTCCA 
CCTCTACCAC CCCGAAACCT AGTTCAGCCG TCTGCTCTTG CAGGACTGAC TCAAGGATCT 
CCCGGCTGGA AGCCTGGCTC TCTGGATCTG AGCGGCAGAC AGAGATCCTC CTCTGACCCT 
CCCAACATGC ATCCTCCTGC GCCTCCCTTA CGGGTCACTT CCACCTCCCT TCTAATGCCC 
AGCGGTGCTG CTCCTCCTCT GGCTAAAGCT ACTGGTATGA TGGAGACCAT GAATATGCAA 
CCCAAACCCG GACAGGGGCC TCCTGGACAG AACATCAACC GGGCTACAAG TGCGGACAAA 
AACTTCAGCA AAAGCACACT GATGCGCTCC GGATCCATCG AGAGACCAGG TAGATGTCAG 
AGAAACCATG TTAACTCTAA AGAAGTCCCA GGAGGCCCAC AAAACACCAC TGGTCAAACT 
CTGCCTGCGA CCCACATGCC CAGGAAAACG TATTTGAAGC CGAAGCGTGT GAAGGCCATG 
TATAACTGTG TGGCCGATAA TCCAGACGAG CTGACCTTCT CTGAGGGAGA GCTTATCGTG 
GTGGATGGAG AGGAGGACCA GGAGTGGTGG CTGGGCCACA TTGAGGGAGA GCCAATGAGA 
AGAGGAGCGT TTCCTGTCAC GTTTGTACAG TTCATTATGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158791.1
CDS	440..3424
Translation	MPDQITVAEFVTETNEDYKSPTASNFTTRMTHCRNTVSALEEALDVDRSVLYKMKKSVKAIYASGLAHVENEEQYTQALEKFGENCVYRDDPDLGSAFLKFSVFTKELTALFKNLFQNMNNIITFPLDSLLKGDLKGVKGDLKKPFDKAWKDYETKVSKIEKEKKEHARQHGMIRTEISGAEIAEEMEKERRFFQLQMCEYLLKVNEIKIKKGVDLLQNLIKYFHAQCNFFQDGLKAVDNLKPSIEKLATDLHSIKQVQDEERRQLTQLRDVLKTALQVEQKESRRDSQVRQSATYSLHQPQGNKEHGTERSGNLYKKSDGLRKVWQKRKCTVKNGYLTISHGTANRPPAKLNLLTCQVKHNPEEKKSFDLISHDRTYHFQAEDEPECQIWISVLQNSKEEALNNAFKGDQHVGENNIVQELTKAILGEVKRMAGNDVCCDCGAPGPTWLSTNLGILTCIECSGIHRELGVHYSRIQSLTLDVLSTSELLLAKNVGNAGFNEIMEACLTAEDVIKPNPASDMQARKDFIMAKYTEKRFARKKCPDALSKLHTLCDAVKARDIFSLIQVYAEGVDLMEPIPLANGHEQGETALHLAVRLVDRTSLHIIDFLTQNSLNLDKQTAKGSTALHYCCLTDNSECLKLLLRGKASIDIANEAGETPLDIARRLKHLQCEELLNQALAGKFNAHVHVEYEWRLQHEDLDESDEDLDEKSSPHRRDERPISCYTPGSNSLQLSPASLSRDGRDLVKDKQRFVPNLVNNETYGTIINTSSPVSLSSSAPPLPPRNLVQPSALAGLTQGSPGWKPGSLDLSGRQRSSSDPPNMHPPAPPLRVTSTSLLMPSGAAPPLAKATGMMETMNMQPKPGQGPPGQNINRATSADKNFSKSTLMRSGSIERPGRCQRNHVNSKEVPGGPQNTTGQTLPATHMPRKTYLKPKRVKAMYNCVADNPDELTFSEGELIVVDGEEDQEWWLGHIEGEPMRRGAFPVTFVQFIMD

Target ORF information:

RefSeq Version	XM_005158734.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2a (asap2a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158734.4

ORF Insert Sequence:

ATGCCTGACC AGATAACAGT GGCGGAGTTT GTCACGGAGA CAAATGAAGA TTATAAATCG 
CCCACCGCCT CAAACTTCAC CACCAGAATG ACTCACTGCA GGAACACAGT ATCCGCACTG 
GAGGAGGCCC TGGATGTGGA CCGCAGTGTC CTTTACAAGA TGAAGAAGTC AGTTAAGGCT 
ATTTACGCCT CGGGTCTGGC TCATGTGGAG AATGAGGAGC AGTACACTCA AGCTCTGGAG 
AAGTTCGGAG AGAACTGTGT GTACAGAGAT GACCCGGACC TGGGATCAGC CTTCCTGAAG 
TTCTCCGTCT TCACCAAGGA GCTCACGGCA CTCTTCAAGA ACCTGTTTCA GAACATGAAT 
AATATCATTA CCTTCCCATT GGACAGTCTG CTGAAGGGAG ATCTGAAAGG GGTTAAAGGG 
GATCTCAAGA AGCCCTTCGA TAAAGCCTGG AAAGACTACG AGACTAAAGT CTCTAAAATA 
GAGAAGGAGA AAAAAGAGCA CGCCCGGCAG CACGGAATGA TCCGGACGGA GATCAGCGGA 
GCAGAGATAG CAGAAGAGAT GGAAAAAGAG CGGCGTTTCT TCCAGCTTCA GATGTGTGAG 
TACCTCCTCA AAGTCAATGA AATCAAGATC AAAAAAGGTG TCGACCTGCT CCAGAATCTC 
ATCAAATACT TCCACGCACA GTGCAACTTC TTTCAGGATG GTCTCAAAGC GGTGGACAAC 
CTCAAACCCT CAATAGAAAA ACTGGCCACA GACTTGCACT CGATCAAACA GGTACAGGAT 
GAAGAACGCA GACAGCTAAC CCAGTTACGG GATGTGCTAA AAACTGCTCT GCAAGTGGAG 
CAGAAGGAGT CTAGGAGAGA CTCTCAGGTT AGACAGAGCG CCACCTACAG TCTGCACCAG 
CCGCAGGGCA ACAAAGAGCA TGGGACTGAG CGCAGCGGCA ACCTTTACAA GAAGAGTGAC 
GGGCTGCGGA AAGTGTGGCA GAAGAGAAAG TGCACAGTAA AGAATGGATA TTTGACCATC 
TCACATGGGA CGGCAAACAG ACCTCCCGCC AAACTCAATC TTCTCACCTG TCAGGTGAAG 
CACAACCCAG AGGAGAAGAA AAGTTTTGAC CTCATCTCAC ATGACAGAAC ATATCATTTC 
CAGGCAGAAG ATGAGCCAGA GTGTCAAATA TGGATCTCAG TGCTGCAGAA CAGTAAAGAA 
GAGGCGCTCA ACAACGCCTT CAAGGGCGAC CAGCATGTTG GTGAAAATAA CATTGTGCAG 
GAGCTCACCA AGGCCATCCT GGGAGAGGTG AAGCGGATGG CGGGGAACGA TGTCTGCTGC 
GACTGCGGTG CTCCCGGCCC CACATGGCTC TCCACCAACC TGGGCATCCT GACCTGCATC 
GAGTGTTCGG GGATCCACAG AGAGCTGGGC GTCCATTACT CCCGAATCCA GTCCCTCACA 
CTCGACGTCC TCAGCACCTC CGAGCTCTTG CTGGCCAAGA ACGTGGGGAA TGCTGGCTTC 
AATGAGATCA TGGAGGCCTG TCTGACGGCA GAAGATGTGA TCAAACCGAA TCCAGCCAGT 
GACATGCAGG CGAGGAAGGA CTTTATCATG GCCAAATACA CAGAGAAACG CTTCGCTCGT 
AAGAAGTGTC CAGACGCACT GTCGAAGCTG CACACGCTCT GTGATGCTGT GAAGGCCCGG 
GACATTTTCT CTCTCATCCA GGTCTATGCT GAAGGAGTGG ATCTGATGGA GCCCATTCCT 
CTGGCTAATG GACATGAACA AGGTGAGACG GCTCTTCATC TGGCCGTGAG ACTGGTGGAC 
AGAACTTCCC TACACATCAT CGACTTCCTC ACCCAAAACA GTTTAAACCT GGATAAGCAA 
ACGGCTAAAG GAAGCACAGC TCTGCATTAC TGCTGCCTGA CGGACAACAG CGAGTGTCTC 
AAACTGCTGC TCAGAGGAAA AGCCTCCATA GATATCGCTA ATGAAGCTGG AGAGACCCCG 
TTGGACATCG CCAGGCGACT CAAACATCTG CAGTGTGAGG AACTGCTGAA CCAGGCTCTT 
GCAGGGAAGT TCAATGCTCA TGTGCATGTG GAGTATGAGT GGAGACTTCA GCATGAAGAC 
CTGGACGAGA GTGATGAAGA TCTGGATGAG AAGTCGAGTC CTCACCGGCG GGATGAGCGG 
CCCATCAGCT GCTACACACC GGGCAGTAAC TCCCTTCAGC TGAGTCCAGC CAGCCTGAGC 
CGAGACGGTC GAGACCTGGT TAAAGACAAG CAACGCTTTG TGCCAAACCT GGTCAACAAT 
GAAACCTACG GGACCATCAT TAACACCAGC TCACCCGTCA GCCTGTCCTC TTCTGCTCCA 
CCTCTACCAC CCCGAAACCT AGTTCAGCCG TCTGCTCTTG CAGGACTGAC TCAAGGATCT 
CCCGGCTGGA AGCCTGGCTC TCTGGATCTG AGCGGCAGAC AGAGATCCTC CTCTGACCCT 
CCCAACATGC ATCCTCCTGC GCCTCCCTTA CGGGTCACTT CCACCTCCCT TCTAATGCCC 
AGCGGTGCTG CTCCTCCTCT GGCTAAAGCT ACTGGTATGA TGGAGACCAT GAATATGCAA 
CCCAAACCCG GACAGGGGCC TCCTGGACAG AACATCAACC GGGCTACAAG TGCGGACAAA 
AACTTCAGCA AAAGCACACT GATGCGCTCC GGATCCATCG AGAGACCAGG TAGATGTCAG 
AGAAACCATG TTAACTCTAA AGAAGTCCCA GGAGGCCCAC AAAACACCAC TGGTCAAACT 
CTGCCTGCGA CCCACATGCC CAGGAAAACG TATTTGAAGC CGAAGCGTGT GAAGGCCATG 
TATAACTGTG TGGCCGATAA TCCAGACGAG CTGACCTTCT CTGAGGGAGA GCTTATCGTG 
GTGGATGGAG AGGAGGACCA GGAGTGGTGG CTGGGCCACA TTGAGGGAGA GCCAATGAGA 
AGAGGAGCGT TTCCTGTCAC GTTTGTACAG TTCATTATGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap2a ( XM_005158734.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005158791.1 Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2a (asap2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa22692
Clone ID Related Accession (Same CDS sequence)	XM_005158734.4 , XM_005158734.3
Accession Version	XM_005158734.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158734.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGACC AGATAACAGT GGCGGAGTTT GTCACGGAGA CAAATGAAGA TTATAAATCG 
CCCACCGCCT CAAACTTCAC CACCAGAATG ACTCACTGCA GGAACACAGT ATCCGCACTG 
GAGGAGGCCC TGGATGTGGA CCGCAGTGTC CTTTACAAGA TGAAGAAGTC AGTTAAGGCT 
ATTTACGCCT CGGGTCTGGC TCATGTGGAG AATGAGGAGC AGTACACTCA AGCTCTGGAG 
AAGTTCGGAG AGAACTGTGT GTACAGAGAT GACCCGGACC TGGGATCAGC CTTCCTGAAG 
TTCTCCGTCT TCACCAAGGA GCTCACGGCA CTCTTCAAGA ACCTGTTTCA GAACATGAAT 
AATATCATTA CCTTCCCATT GGACAGTCTG CTGAAGGGAG ATCTGAAAGG GGTTAAAGGG 
GATCTCAAGA AGCCCTTCGA TAAAGCCTGG AAAGACTACG AGACTAAAGT CTCTAAAATA 
GAGAAGGAGA AAAAAGAGCA CGCCCGGCAG CACGGAATGA TCCGGACGGA GATCAGCGGA 
GCAGAGATAG CAGAAGAGAT GGAAAAAGAG CGGCGTTTCT TCCAGCTTCA GATGTGTGAG 
TACCTCCTCA AAGTCAATGA AATCAAGATC AAAAAAGGTG TCGACCTGCT CCAGAATCTC 
ATCAAATACT TCCACGCACA GTGCAACTTC TTTCAGGATG GTCTCAAAGC GGTGGACAAC 
CTCAAACCCT CAATAGAAAA ACTGGCCACA GACTTGCACT CGATCAAACA GGTACAGGAT 
GAAGAACGCA GACAGCTAAC CCAGTTACGG GATGTGCTAA AAACTGCTCT GCAAGTGGAG 
CAGAAGGAGT CTAGGAGAGA CTCTCAGGTT AGACAGAGCG CCACCTACAG TCTGCACCAG 
CCGCAGGGCA ACAAAGAGCA TGGGACTGAG CGCAGCGGCA ACCTTTACAA GAAGAGTGAC 
GGGCTGCGGA AAGTGTGGCA GAAGAGAAAG TGCACAGTAA AGAATGGATA TTTGACCATC 
TCACATGGGA CGGCAAACAG ACCTCCCGCC AAACTCAATC TTCTCACCTG TCAGGTGAAG 
CACAACCCAG AGGAGAAGAA AAGTTTTGAC CTCATCTCAC ATGACAGAAC ATATCATTTC 
CAGGCAGAAG ATGAGCCAGA GTGTCAAATA TGGATCTCAG TGCTGCAGAA CAGTAAAGAA 
GAGGCGCTCA ACAACGCCTT CAAGGGCGAC CAGCATGTTG GTGAAAATAA CATTGTGCAG 
GAGCTCACCA AGGCCATCCT GGGAGAGGTG AAGCGGATGG CGGGGAACGA TGTCTGCTGC 
GACTGCGGTG CTCCCGGCCC CACATGGCTC TCCACCAACC TGGGCATCCT GACCTGCATC 
GAGTGTTCGG GGATCCACAG AGAGCTGGGC GTCCATTACT CCCGAATCCA GTCCCTCACA 
CTCGACGTCC TCAGCACCTC CGAGCTCTTG CTGGCCAAGA ACGTGGGGAA TGCTGGCTTC 
AATGAGATCA TGGAGGCCTG TCTGACGGCA GAAGATGTGA TCAAACCGAA TCCAGCCAGT 
GACATGCAGG CGAGGAAGGA CTTTATCATG GCCAAATACA CAGAGAAACG CTTCGCTCGT 
AAGAAGTGTC CAGACGCACT GTCGAAGCTG CACACGCTCT GTGATGCTGT GAAGGCCCGG 
GACATTTTCT CTCTCATCCA GGTCTATGCT GAAGGAGTGG ATCTGATGGA GCCCATTCCT 
CTGGCTAATG GACATGAACA AGGTGAGACG GCTCTTCATC TGGCCGTGAG ACTGGTGGAC 
AGAACTTCCC TACACATCAT CGACTTCCTC ACCCAAAACA GTTTAAACCT GGATAAGCAA 
ACGGCTAAAG GAAGCACAGC TCTGCATTAC TGCTGCCTGA CGGACAACAG CGAGTGTCTC 
AAACTGCTGC TCAGAGGAAA AGCCTCCATA GATATCGCTA ATGAAGCTGG AGAGACCCCG 
TTGGACATCG CCAGGCGACT CAAACATCTG CAGTGTGAGG AACTGCTGAA CCAGGCTCTT 
GCAGGGAAGT TCAATGCTCA TGTGCATGTG GAGTATGAGT GGAGACTTCA GCATGAAGAC 
CTGGACGAGA GTGATGAAGA TCTGGATGAG AAGTCGAGTC CTCACCGGCG GGATGAGCGG 
CCCATCAGCT GCTACACACC GGGCAGTAAC TCCCTTCAGC TGAGTCCAGC CAGCCTGAGC 
CGAGACGGTC GAGACCTGGT TAAAGACAAG CAACGCTTTG TGCCAAACCT GGTCAACAAT 
GAAACCTACG GGACCATCAT TAACACCAGC TCACCCGTCA GCCTGTCCTC TTCTGCTCCA 
CCTCTACCAC CCCGAAACCT AGTTCAGCCG TCTGCTCTTG CAGGACTGAC TCAAGGATCT 
CCCGGCTGGA AGCCTGGCTC TCTGGATCTG AGCGGCAGAC AGAGATCCTC CTCTGACCCT 
CCCAACATGC ATCCTCCTGC GCCTCCCTTA CGGGTCACTT CCACCTCCCT TCTAATGCCC 
AGCGGTGCTG CTCCTCCTCT GGCTAAAGCT ACTGGTATGA TGGAGACCAT GAATATGCAA 
CCCAAACCCG GACAGGGGCC TCCTGGACAG AACATCAACC GGGCTACAAG TGCGGACAAA 
AACTTCAGCA AAAGCACACT GATGCGCTCC GGATCCATCG AGAGACCAGG TAGATGTCAG 
AGAAACCATG TTAACTCTAA AGAAGTCCCA GGAGGCCCAC AAAACACCAC TGGTCAAACT 
CTGCCTGCGA CCCACATGCC CAGGAAAACG TATTTGAAGC CGAAGCGTGT GAAGGCCATG 
TATAACTGTG TGGCCGATAA TCCAGACGAG CTGACCTTCT CTGAGGGAGA GCTTATCGTG 
GTGGATGGAG AGGAGGACCA GGAGTGGTGG CTGGGCCACA TTGAGGGAGA GCCAATGAGA 
AGAGGAGCGT TTCCTGTCAC GTTTGTACAG TTCATTATGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005158791.1
CDS	428..3412
Translation	MPDQITVAEFVTETNEDYKSPTASNFTTRMTHCRNTVSALEEALDVDRSVLYKMKKSVKAIYASGLAHVENEEQYTQALEKFGENCVYRDDPDLGSAFLKFSVFTKELTALFKNLFQNMNNIITFPLDSLLKGDLKGVKGDLKKPFDKAWKDYETKVSKIEKEKKEHARQHGMIRTEISGAEIAEEMEKERRFFQLQMCEYLLKVNEIKIKKGVDLLQNLIKYFHAQCNFFQDGLKAVDNLKPSIEKLATDLHSIKQVQDEERRQLTQLRDVLKTALQVEQKESRRDSQVRQSATYSLHQPQGNKEHGTERSGNLYKKSDGLRKVWQKRKCTVKNGYLTISHGTANRPPAKLNLLTCQVKHNPEEKKSFDLISHDRTYHFQAEDEPECQIWISVLQNSKEEALNNAFKGDQHVGENNIVQELTKAILGEVKRMAGNDVCCDCGAPGPTWLSTNLGILTCIECSGIHRELGVHYSRIQSLTLDVLSTSELLLAKNVGNAGFNEIMEACLTAEDVIKPNPASDMQARKDFIMAKYTEKRFARKKCPDALSKLHTLCDAVKARDIFSLIQVYAEGVDLMEPIPLANGHEQGETALHLAVRLVDRTSLHIIDFLTQNSLNLDKQTAKGSTALHYCCLTDNSECLKLLLRGKASIDIANEAGETPLDIARRLKHLQCEELLNQALAGKFNAHVHVEYEWRLQHEDLDESDEDLDEKSSPHRRDERPISCYTPGSNSLQLSPASLSRDGRDLVKDKQRFVPNLVNNETYGTIINTSSPVSLSSSAPPLPPRNLVQPSALAGLTQGSPGWKPGSLDLSGRQRSSSDPPNMHPPAPPLRVTSTSLLMPSGAAPPLAKATGMMETMNMQPKPGQGPPGQNINRATSADKNFSKSTLMRSGSIERPGRCQRNHVNSKEVPGGPQNTTGQTLPATHMPRKTYLKPKRVKAMYNCVADNPDELTFSEGELIVVDGEEDQEWWLGHIEGEPMRRGAFPVTFVQFIMD

Target ORF information:

RefSeq Version	XM_005158734.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2a (asap2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158734.3

ORF Insert Sequence:

ATGCCTGACC AGATAACAGT GGCGGAGTTT GTCACGGAGA CAAATGAAGA TTATAAATCG 
CCCACCGCCT CAAACTTCAC CACCAGAATG ACTCACTGCA GGAACACAGT ATCCGCACTG 
GAGGAGGCCC TGGATGTGGA CCGCAGTGTC CTTTACAAGA TGAAGAAGTC AGTTAAGGCT 
ATTTACGCCT CGGGTCTGGC TCATGTGGAG AATGAGGAGC AGTACACTCA AGCTCTGGAG 
AAGTTCGGAG AGAACTGTGT GTACAGAGAT GACCCGGACC TGGGATCAGC CTTCCTGAAG 
TTCTCCGTCT TCACCAAGGA GCTCACGGCA CTCTTCAAGA ACCTGTTTCA GAACATGAAT 
AATATCATTA CCTTCCCATT GGACAGTCTG CTGAAGGGAG ATCTGAAAGG GGTTAAAGGG 
GATCTCAAGA AGCCCTTCGA TAAAGCCTGG AAAGACTACG AGACTAAAGT CTCTAAAATA 
GAGAAGGAGA AAAAAGAGCA CGCCCGGCAG CACGGAATGA TCCGGACGGA GATCAGCGGA 
GCAGAGATAG CAGAAGAGAT GGAAAAAGAG CGGCGTTTCT TCCAGCTTCA GATGTGTGAG 
TACCTCCTCA AAGTCAATGA AATCAAGATC AAAAAAGGTG TCGACCTGCT CCAGAATCTC 
ATCAAATACT TCCACGCACA GTGCAACTTC TTTCAGGATG GTCTCAAAGC GGTGGACAAC 
CTCAAACCCT CAATAGAAAA ACTGGCCACA GACTTGCACT CGATCAAACA GGTACAGGAT 
GAAGAACGCA GACAGCTAAC CCAGTTACGG GATGTGCTAA AAACTGCTCT GCAAGTGGAG 
CAGAAGGAGT CTAGGAGAGA CTCTCAGGTT AGACAGAGCG CCACCTACAG TCTGCACCAG 
CCGCAGGGCA ACAAAGAGCA TGGGACTGAG CGCAGCGGCA ACCTTTACAA GAAGAGTGAC 
GGGCTGCGGA AAGTGTGGCA GAAGAGAAAG TGCACAGTAA AGAATGGATA TTTGACCATC 
TCACATGGGA CGGCAAACAG ACCTCCCGCC AAACTCAATC TTCTCACCTG TCAGGTGAAG 
CACAACCCAG AGGAGAAGAA AAGTTTTGAC CTCATCTCAC ATGACAGAAC ATATCATTTC 
CAGGCAGAAG ATGAGCCAGA GTGTCAAATA TGGATCTCAG TGCTGCAGAA CAGTAAAGAA 
GAGGCGCTCA ACAACGCCTT CAAGGGCGAC CAGCATGTTG GTGAAAATAA CATTGTGCAG 
GAGCTCACCA AGGCCATCCT GGGAGAGGTG AAGCGGATGG CGGGGAACGA TGTCTGCTGC 
GACTGCGGTG CTCCCGGCCC CACATGGCTC TCCACCAACC TGGGCATCCT GACCTGCATC 
GAGTGTTCGG GGATCCACAG AGAGCTGGGC GTCCATTACT CCCGAATCCA GTCCCTCACA 
CTCGACGTCC TCAGCACCTC CGAGCTCTTG CTGGCCAAGA ACGTGGGGAA TGCTGGCTTC 
AATGAGATCA TGGAGGCCTG TCTGACGGCA GAAGATGTGA TCAAACCGAA TCCAGCCAGT 
GACATGCAGG CGAGGAAGGA CTTTATCATG GCCAAATACA CAGAGAAACG CTTCGCTCGT 
AAGAAGTGTC CAGACGCACT GTCGAAGCTG CACACGCTCT GTGATGCTGT GAAGGCCCGG 
GACATTTTCT CTCTCATCCA GGTCTATGCT GAAGGAGTGG ATCTGATGGA GCCCATTCCT 
CTGGCTAATG GACATGAACA AGGTGAGACG GCTCTTCATC TGGCCGTGAG ACTGGTGGAC 
AGAACTTCCC TACACATCAT CGACTTCCTC ACCCAAAACA GTTTAAACCT GGATAAGCAA 
ACGGCTAAAG GAAGCACAGC TCTGCATTAC TGCTGCCTGA CGGACAACAG CGAGTGTCTC 
AAACTGCTGC TCAGAGGAAA AGCCTCCATA GATATCGCTA ATGAAGCTGG AGAGACCCCG 
TTGGACATCG CCAGGCGACT CAAACATCTG CAGTGTGAGG AACTGCTGAA CCAGGCTCTT 
GCAGGGAAGT TCAATGCTCA TGTGCATGTG GAGTATGAGT GGAGACTTCA GCATGAAGAC 
CTGGACGAGA GTGATGAAGA TCTGGATGAG AAGTCGAGTC CTCACCGGCG GGATGAGCGG 
CCCATCAGCT GCTACACACC GGGCAGTAAC TCCCTTCAGC TGAGTCCAGC CAGCCTGAGC 
CGAGACGGTC GAGACCTGGT TAAAGACAAG CAACGCTTTG TGCCAAACCT GGTCAACAAT 
GAAACCTACG GGACCATCAT TAACACCAGC TCACCCGTCA GCCTGTCCTC TTCTGCTCCA 
CCTCTACCAC CCCGAAACCT AGTTCAGCCG TCTGCTCTTG CAGGACTGAC TCAAGGATCT 
CCCGGCTGGA AGCCTGGCTC TCTGGATCTG AGCGGCAGAC AGAGATCCTC CTCTGACCCT 
CCCAACATGC ATCCTCCTGC GCCTCCCTTA CGGGTCACTT CCACCTCCCT TCTAATGCCC 
AGCGGTGCTG CTCCTCCTCT GGCTAAAGCT ACTGGTATGA TGGAGACCAT GAATATGCAA 
CCCAAACCCG GACAGGGGCC TCCTGGACAG AACATCAACC GGGCTACAAG TGCGGACAAA 
AACTTCAGCA AAAGCACACT GATGCGCTCC GGATCCATCG AGAGACCAGG TAGATGTCAG 
AGAAACCATG TTAACTCTAA AGAAGTCCCA GGAGGCCCAC AAAACACCAC TGGTCAAACT 
CTGCCTGCGA CCCACATGCC CAGGAAAACG TATTTGAAGC CGAAGCGTGT GAAGGCCATG 
TATAACTGTG TGGCCGATAA TCCAGACGAG CTGACCTTCT CTGAGGGAGA GCTTATCGTG 
GTGGATGGAG AGGAGGACCA GGAGTGGTGG CTGGGCCACA TTGAGGGAGA GCCAATGAGA 
AGAGGAGCGT TTCCTGTCAC GTTTGTACAG TTCATTATGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap2a ( XM_021467219.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322894.1 Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2a (asap2a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa149579
Clone ID Related Accession (Same CDS sequence)	XM_021467219.1
Accession Version	XM_021467219.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGACC AGATAACAGT GGCGGAGTTT GTCACGGAGA CAAATGAAGA TTATAAATCG 
CCCACCGCCT CAAACTTCAC CACCAGAATG ACTCACTGCA GGAACACAGT ATCCGCACTG 
GAGGAGGCCC TGGATGTGGA CCGCAGTGTC CTTTACAAGA TGAAGAAGTC AGTTAAGGCT 
ATTTACGCCT CGGGTCTGGC TCATGTGGAG AATGAGGAGC AGTACACTCA AGCTCTGGAG 
AAGTTCGGAG AGAACTGTGT GTACAGAGAT GACCCGGACC TGGGATCAGC CTTCCTGAAG 
TTCTCCGTCT TCACCAAGGA GCTCACGGCA CTCTTCAAGA ACCTGTTTCA GAACATGAAT 
AATATCATTA CCTTCCCATT GGACAGTCTG CTGAAGGGAG ATCTGAAAGG GGTTAAAGGG 
GATCTCAAGA AGCCCTTCGA TAAAGCCTGG AAAGACTACG AGACTAAAGT CTGCAAGGGA 
CATTCTTTAC TGAAAGGCTC TAAAATAGAG AAGGAGAAAA AAGAGCACGC CCGGCAGCAC 
GGAATGATCC GGACGGAGAT CAGCGGAGCA GAGATAGCAG AAGAGATGGA AAAAGAGCGG 
CGTTTCTTCC AGCTTCAGAT GTGTGAGTAC CTCCTCAAAG TCAATGAAAT CAAGATCAAA 
AAAGGTGTCG ACCTGCTCCA GAATCTCATC AAATACTTCC ACGCACAGTG CAACTTCTTT 
CAGGATGGTC TCAAAGCGGT GGACAACCTC AAACCCTCAA TAGAAAAACT GGCCACAGAC 
TTGCACTCGA TCAAACAGGT ACAGGATGAA GAACGCAGAC AGCTAACCCA GTTACGGGAT 
GTGCTAAAAA CTGCTCTGCA AGTGGAGCAG AAGGAGTCTA GGAGAGACTC TCAGGTTAGA 
CAGAGCGCCA CCTACAGTCT GCACCAGCCG CAGGGCAACA AAGAGCATGG GACTGAGCGC 
AGCGGCAACC TTTACAAGAA GAGTGACGGG CTGCGGAAAG TGTGGCAGAA GAGAAAGTGC 
ACAGTAAAGA ATGGATATTT GACCATCTCA CATGGGACGG CAAACAGACC TCCCGCCAAA 
CTCAATCTTC TCACCTGTCA GGTGAAGCAC AACCCAGAGG AGAAGAAAAG TTTTGACCTC 
ATCTCACATG ACAGAACATA TCATTTCCAG GCAGAAGATG AGCCAGAGTG TCAAATATGG 
ATCTCAGTGC TGCAGAACAG TAAAGAAGAG GCGCTCAACA ACGCCTTCAA GGGCGACCAG 
CATGTTGGTG AAAATAACAT TGTGCAGGAG CTCACCAAGG CCATCCTGGG AGAGGTGAAG 
CGGATGGCGG GGAACGATGT CTGCTGCGAC TGCGGTGCTC CCGGCCCCAC ATGGCTCTCC 
ACCAACCTGG GCATCCTGAC CTGCATCGAG TGTTCGGGGA TCCACAGAGA GCTGGGCGTC 
CATTACTCCC GAATCCAGTC CCTCACACTC GACGTCCTCA GCACCTCCGA GCTCTTGCTG 
GCCAAGAACG TGGGGAATGC TGGCTTCAAT GAGATCATGG AGGCCTGTCT GACGGCAGAA 
GATGTGATCA AACCGAATCC AGCCAGTGAC ATGCAGGCGA GGAAGGACTT TATCATGGCC 
AAATACACAG AGAAACGCTT CGCTCGTAAG AAGTGTCCAG ACGCACTGTC GAAGCTGCAC 
ACGCTCTGTG ATGCTGTGAA GGCCCGGGAC ATTTTCTCTC TCATCCAGGT CTATGCTGAA 
GGAGTGGATC TGATGGAGCC CATTCCTCTG GCTAATGGAC ATGAACAAGG TGAGACGGCT 
CTTCATCTGG CCGTGAGACT GGTGGACAGA ACTTCCCTAC ACATCATCGA CTTCCTCACC 
CAAAACAGTT TAAACCTGGA TAAGCAAACG GCTAAAGGAA GCACAGCTCT GCATTACTGC 
TGCCTGACGG ACAACAGCGA GTGTCTCAAA CTGCTGCTCA GAGGAAAAGC CTCCATAGAT 
ATCGCTAATG AAGCTGGAGA GACCCCGTTG GACATCGCCA GGCGACTCAA ACATCTGCAG 
TGTGAGGAAC TGCTGAACCA GGCTCTTGCA GGGAAGTTCA ATGCTCATGT GCATGTGGAG 
TATGAGTGGA GACTTCAGCA TGAAGACCTG GACGAGAGTG ATGAAGATCT GGATGAGAAG 
TCGAGTCCTC ACCGGCGGGA TGAGCGGCCC ATCAGCTGCT ACACACCGGG CAGTAACTCC 
CTTCAGCTGA GTCCAGCCAG CCTGAGCCGA GACGGTCGAG ACCTGGTTAA AGACAAGCAA 
CGCTTTGTGC CAAACCTGGT CAACAATGAA ACCTACGGGA CCATCATTAA CACCAGCTCA 
CCCGTCAGCC TGTCCTCTTC TGCTCCACCT CTACCACCCC GAAACCTAGT TCAGCCGTCT 
GCTCTTGCAG GACTGACTCA AGGATCTCCC GGCTGGAAGC CTGGCTCTCT GGATCTGAGC 
GGCAGACAGA GATCCTCCTC TGACCCTCCC AACATGCATC CTCCTGCGCC TCCCTTACGG 
GTCACTTCCA CCTCCCTTCT AATGCCCAGC GGTGCTGCTC CTCCTCTGGC TAAAGCTACT 
GGTATGATGG AGACCATGAA TATGCAACCC AAACCCGGAC AGGGGCCTCC TGGACAGAAC 
ATCAACCGGG CTACAAGTGC GGACAAAAAC TTCAGCAAAA GCACACTGAT GCGCTCCGGA 
TCCATCGAGA GACCAGCTAA AGAAGTCCCA GGAGGCCCAC AAAACACCAC TGGTCAAACT 
CTGCCTGCGA CCCACATGCC CAGGAAAACG TATTTGAAGC CGAAGCGTGT GAAGGCCATG 
TATAACTGTG TGGCCGATAA TCCAGACGAG CTGACCTTCT CTGAGGGAGA GCTTATCGTG 
GTGGATGGAG AGGAGGACCA GGAGTGGTGG CTGGGCCACA TTGAGGGAGA GCCAATGAGA 
AGAGGAGCGT TTCCTGTCAC GTTTGTACAG TTCATTATGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322894.1
CDS	440..3424
Translation	MPDQITVAEFVTETNEDYKSPTASNFTTRMTHCRNTVSALEEALDVDRSVLYKMKKSVKAIYASGLAHVENEEQYTQALEKFGENCVYRDDPDLGSAFLKFSVFTKELTALFKNLFQNMNNIITFPLDSLLKGDLKGVKGDLKKPFDKAWKDYETKVCKGHSLLKGSKIEKEKKEHARQHGMIRTEISGAEIAEEMEKERRFFQLQMCEYLLKVNEIKIKKGVDLLQNLIKYFHAQCNFFQDGLKAVDNLKPSIEKLATDLHSIKQVQDEERRQLTQLRDVLKTALQVEQKESRRDSQVRQSATYSLHQPQGNKEHGTERSGNLYKKSDGLRKVWQKRKCTVKNGYLTISHGTANRPPAKLNLLTCQVKHNPEEKKSFDLISHDRTYHFQAEDEPECQIWISVLQNSKEEALNNAFKGDQHVGENNIVQELTKAILGEVKRMAGNDVCCDCGAPGPTWLSTNLGILTCIECSGIHRELGVHYSRIQSLTLDVLSTSELLLAKNVGNAGFNEIMEACLTAEDVIKPNPASDMQARKDFIMAKYTEKRFARKKCPDALSKLHTLCDAVKARDIFSLIQVYAEGVDLMEPIPLANGHEQGETALHLAVRLVDRTSLHIIDFLTQNSLNLDKQTAKGSTALHYCCLTDNSECLKLLLRGKASIDIANEAGETPLDIARRLKHLQCEELLNQALAGKFNAHVHVEYEWRLQHEDLDESDEDLDEKSSPHRRDERPISCYTPGSNSLQLSPASLSRDGRDLVKDKQRFVPNLVNNETYGTIINTSSPVSLSSSAPPLPPRNLVQPSALAGLTQGSPGWKPGSLDLSGRQRSSSDPPNMHPPAPPLRVTSTSLLMPSGAAPPLAKATGMMETMNMQPKPGQGPPGQNINRATSADKNFSKSTLMRSGSIERPAKEVPGGPQNTTGQTLPATHMPRKTYLKPKRVKAMYNCVADNPDELTFSEGELIVVDGEEDQEWWLGHIEGEPMRRGAFPVTFVQFIMD

Target ORF information:

RefSeq Version	XM_021467219.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2a (asap2a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467219.1

ORF Insert Sequence:

ATGCCTGACC AGATAACAGT GGCGGAGTTT GTCACGGAGA CAAATGAAGA TTATAAATCG 
CCCACCGCCT CAAACTTCAC CACCAGAATG ACTCACTGCA GGAACACAGT ATCCGCACTG 
GAGGAGGCCC TGGATGTGGA CCGCAGTGTC CTTTACAAGA TGAAGAAGTC AGTTAAGGCT 
ATTTACGCCT CGGGTCTGGC TCATGTGGAG AATGAGGAGC AGTACACTCA AGCTCTGGAG 
AAGTTCGGAG AGAACTGTGT GTACAGAGAT GACCCGGACC TGGGATCAGC CTTCCTGAAG 
TTCTCCGTCT TCACCAAGGA GCTCACGGCA CTCTTCAAGA ACCTGTTTCA GAACATGAAT 
AATATCATTA CCTTCCCATT GGACAGTCTG CTGAAGGGAG ATCTGAAAGG GGTTAAAGGG 
GATCTCAAGA AGCCCTTCGA TAAAGCCTGG AAAGACTACG AGACTAAAGT CTGCAAGGGA 
CATTCTTTAC TGAAAGGCTC TAAAATAGAG AAGGAGAAAA AAGAGCACGC CCGGCAGCAC 
GGAATGATCC GGACGGAGAT CAGCGGAGCA GAGATAGCAG AAGAGATGGA AAAAGAGCGG 
CGTTTCTTCC AGCTTCAGAT GTGTGAGTAC CTCCTCAAAG TCAATGAAAT CAAGATCAAA 
AAAGGTGTCG ACCTGCTCCA GAATCTCATC AAATACTTCC ACGCACAGTG CAACTTCTTT 
CAGGATGGTC TCAAAGCGGT GGACAACCTC AAACCCTCAA TAGAAAAACT GGCCACAGAC 
TTGCACTCGA TCAAACAGGT ACAGGATGAA GAACGCAGAC AGCTAACCCA GTTACGGGAT 
GTGCTAAAAA CTGCTCTGCA AGTGGAGCAG AAGGAGTCTA GGAGAGACTC TCAGGTTAGA 
CAGAGCGCCA CCTACAGTCT GCACCAGCCG CAGGGCAACA AAGAGCATGG GACTGAGCGC 
AGCGGCAACC TTTACAAGAA GAGTGACGGG CTGCGGAAAG TGTGGCAGAA GAGAAAGTGC 
ACAGTAAAGA ATGGATATTT GACCATCTCA CATGGGACGG CAAACAGACC TCCCGCCAAA 
CTCAATCTTC TCACCTGTCA GGTGAAGCAC AACCCAGAGG AGAAGAAAAG TTTTGACCTC 
ATCTCACATG ACAGAACATA TCATTTCCAG GCAGAAGATG AGCCAGAGTG TCAAATATGG 
ATCTCAGTGC TGCAGAACAG TAAAGAAGAG GCGCTCAACA ACGCCTTCAA GGGCGACCAG 
CATGTTGGTG AAAATAACAT TGTGCAGGAG CTCACCAAGG CCATCCTGGG AGAGGTGAAG 
CGGATGGCGG GGAACGATGT CTGCTGCGAC TGCGGTGCTC CCGGCCCCAC ATGGCTCTCC 
ACCAACCTGG GCATCCTGAC CTGCATCGAG TGTTCGGGGA TCCACAGAGA GCTGGGCGTC 
CATTACTCCC GAATCCAGTC CCTCACACTC GACGTCCTCA GCACCTCCGA GCTCTTGCTG 
GCCAAGAACG TGGGGAATGC TGGCTTCAAT GAGATCATGG AGGCCTGTCT GACGGCAGAA 
GATGTGATCA AACCGAATCC AGCCAGTGAC ATGCAGGCGA GGAAGGACTT TATCATGGCC 
AAATACACAG AGAAACGCTT CGCTCGTAAG AAGTGTCCAG ACGCACTGTC GAAGCTGCAC 
ACGCTCTGTG ATGCTGTGAA GGCCCGGGAC ATTTTCTCTC TCATCCAGGT CTATGCTGAA 
GGAGTGGATC TGATGGAGCC CATTCCTCTG GCTAATGGAC ATGAACAAGG TGAGACGGCT 
CTTCATCTGG CCGTGAGACT GGTGGACAGA ACTTCCCTAC ACATCATCGA CTTCCTCACC 
CAAAACAGTT TAAACCTGGA TAAGCAAACG GCTAAAGGAA GCACAGCTCT GCATTACTGC 
TGCCTGACGG ACAACAGCGA GTGTCTCAAA CTGCTGCTCA GAGGAAAAGC CTCCATAGAT 
ATCGCTAATG AAGCTGGAGA GACCCCGTTG GACATCGCCA GGCGACTCAA ACATCTGCAG 
TGTGAGGAAC TGCTGAACCA GGCTCTTGCA GGGAAGTTCA ATGCTCATGT GCATGTGGAG 
TATGAGTGGA GACTTCAGCA TGAAGACCTG GACGAGAGTG ATGAAGATCT GGATGAGAAG 
TCGAGTCCTC ACCGGCGGGA TGAGCGGCCC ATCAGCTGCT ACACACCGGG CAGTAACTCC 
CTTCAGCTGA GTCCAGCCAG CCTGAGCCGA GACGGTCGAG ACCTGGTTAA AGACAAGCAA 
CGCTTTGTGC CAAACCTGGT CAACAATGAA ACCTACGGGA CCATCATTAA CACCAGCTCA 
CCCGTCAGCC TGTCCTCTTC TGCTCCACCT CTACCACCCC GAAACCTAGT TCAGCCGTCT 
GCTCTTGCAG GACTGACTCA AGGATCTCCC GGCTGGAAGC CTGGCTCTCT GGATCTGAGC 
GGCAGACAGA GATCCTCCTC TGACCCTCCC AACATGCATC CTCCTGCGCC TCCCTTACGG 
GTCACTTCCA CCTCCCTTCT AATGCCCAGC GGTGCTGCTC CTCCTCTGGC TAAAGCTACT 
GGTATGATGG AGACCATGAA TATGCAACCC AAACCCGGAC AGGGGCCTCC TGGACAGAAC 
ATCAACCGGG CTACAAGTGC GGACAAAAAC TTCAGCAAAA GCACACTGAT GCGCTCCGGA 
TCCATCGAGA GACCAGCTAA AGAAGTCCCA GGAGGCCCAC AAAACACCAC TGGTCAAACT 
CTGCCTGCGA CCCACATGCC CAGGAAAACG TATTTGAAGC CGAAGCGTGT GAAGGCCATG 
TATAACTGTG TGGCCGATAA TCCAGACGAG CTGACCTTCT CTGAGGGAGA GCTTATCGTG 
GTGGATGGAG AGGAGGACCA GGAGTGGTGG CTGGGCCACA TTGAGGGAGA GCCAATGAGA 
AGAGGAGCGT TTCCTGTCAC GTTTGTACAG TTCATTATGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap2a ( XM_017351668.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207157.1 Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2a (asap2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa55607
Clone ID Related Accession (Same CDS sequence)	XM_017351668.1
Accession Version	XM_017351668.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGACC AGATAACAGT GGCGGAGTTT GTCACGGAGA CAAATGAAGA TTATAAATCG 
CCCACCGCCT CAAACTTCAC CACCAGAATG ACTCACTGCA GGAACACAGT ATCCGCACTG 
GAGGAGGCCC TGGATGTGGA CCGCAGTGTC CTTTACAAGA TGAAGAAGTC AGTTAAGGCT 
ATTTACGCCT CGGGTCTGGC TCATGTGGAG AATGAGGAGC AGTACACTCA AGCTCTGGAG 
AAGTTCGGAG AGAACTGTGT GTACAGAGAT GACCCGGACC TGGGATCAGC CTTCCTGAAG 
TTCTCCGTCT TCACCAAGGA GCTCACGGCA CTCTTCAAGA ACCTGTTTCA GAACATGAAT 
AATATCATTA CCTTCCCATT GGACAGTCTG CTGAAGGGAG ATCTGAAAGG GGTTAAAGGG 
GATCTCAAGA AGCCCTTCGA TAAAGCCTGG AAAGACTACG AGACTAAAGT CTCTAAAATA 
GAGAAGGAGA AAAAAGAGCA CGCCCGGCAG CACGGAATGA TCCGGACGGA GATCAGCGGA 
GCAGAGATAG CAGAAGAGAT GGAAAAAGAG CGGCGTTTCT TCCAGCTTCA GATGTGTGAG 
TACCTCCTCA AAGTCAATGA AATCAAGATC AAAAAAGGTG TCGACCTGCT CCAGAATCTC 
ATCAAATACT TCCACGCACA GTGCAACTTC TTTCAGGATG GTCTCAAAGC GGTGGACAAC 
CTCAAACCCT CAATAGAAAA ACTGGCCACA GACTTGCACT CGATCAAACA GGTACAGGAT 
GAAGAACGCA GACAGCTAAC CCAGTTACGG GATGTGCTAA AAACTGCTCT GCAAGTGGAG 
CAGAAGGAGT CTAGGAGAGA CTCTCAGGTT AGACAGAGCG CCACCTACAG TCTGCACCAG 
CCGCAGGGCA ACAAAGAGCA TGGGACTGAG CGCAGCGGCA ACCTTTACAA GAAGAGTGAC 
GGGCTGCGGA AAGTGTGGCA GAAGAGAAAG TGCACAGTAA AGAATGGATA TTTGACCATC 
TCACATGGGA CGGCAAACAG ACCTCCCGCC AAACTCAATC TTCTCACCTG TCAGGTGAAG 
CACAACCCAG AGGAGAAGAA AAGTTTTGAC CTCATCTCAC ATGACAGAAC ATATCATTTC 
CAGGCAGAAG ATGAGCCAGA GTGTCAAATA TGGATCTCAG TGCTGCAGAA CAGTAAAGAA 
GAGGCGCTCA ACAACGCCTT CAAGGGCGAC CAGCATGTTG GTGAAAATAA CATTGTGCAG 
GAGCTCACCA AGGCCATCCT GGGAGAGGTG AAGCGGATGG CGGGGAACGA TGTCTGCTGC 
GACTGCGGTG CTCCCGGCCC CACATGGCTC TCCACCAACC TGGGCATCCT GACCTGCATC 
GAGTGTTCGG GGATCCACAG AGAGCTGGGC GTCCATTACT CCCGAATCCA GTCCCTCACA 
CTCGACGTCC TCAGCACCTC CGAGCTCTTG CTGGCCAAGA ACGTGGGGAA TGCTGGCTTC 
AATGAGATCA TGGAGGCCTG TCTGACGGCA GAAGATGTGA TCAAACCGAA TCCAGCCAGT 
GACATGCAGG CGAGGAAGGA CTTTATCATG GCCAAATACA CAGAGAAACG CTTCGCTCGT 
AAGAAGTGTC CAGACGCACT GTCGAAGCTG CACACGCTCT GTGATGCTGT GAAGGCCCGG 
GACATTTTCT CTCTCATCCA GGTCTATGCT GAAGGAGTGG ATCTGATGGA GCCCATTCCT 
CTGGCTAATG GACATGAACA AGGTGAGACG GCTCTTCATC TGGCCGTGAG ACTGGTGGAC 
AGAACTTCCC TACACATCAT CGACTTCCTC ACCCAAAACA GTTTAAACCT GGATAAGCAA 
ACGGCTAAAG GAAGCACAGC TCTGCATTAC TGCTGCCTGA CGGACAACAG CGAGTGTCTC 
AAACTGCTGC TCAGAGGAAA AGCCTCCATA GATATCGCTA ATGAAGCTGG AGAGACCCCG 
TTGGACATCG CCAGGCGACT CAAACATCTG CAGTGTGAGG AACTGCTGAA CCAGGCTCTT 
GCAGGGAAGT TCAATGCTCA TGTGCATGTG GAGTATGAGT GGAGACTTCA GCATGAAGAC 
CTGGACGAGA GTGATGAAGA TCTGGATGAG AAGTCGAGTC CTCACCGGCG GGATGAGCGG 
CCCATCAGCT GCTACACACC GGGCAGTAAC TCCCTTCAGC TGAGTCCAGC CAGCCTGAGC 
CGAGACGGTC GAGACCTGGT TAAAGACAAG CAACGCTTTG TGCCAAACCT GGTCAACAAT 
GAAACCTACG GGACCATCAT TAACACCAGC TCACCCGTCA GCCTGTCCTC TTCTGCTCCA 
CCTCTACCAC CCCGAAACCT AGTTCAGCCG TCTGCTCTTG CAGGACTGAC TCAAGGATCT 
CCCGGCTGGA AGCCTGGCTC TCTGGATCTG AGCGGCAGAC AGAGATCCTC CTCTGACCCT 
CCCAACATGC ATCCTCCTGC GCCTCCCTTA CGGGTCACTT CCACCTCCCT TCTAATGCCC 
AGCGGTGCTG CTCCTCCTCT GGCTAAAGCT ACTGGTATGA TGGAGACCAT GAATATGCAA 
CCCAAACCCG GACAGGGGCC TCCTGGACAG AACATCAACC GGGCTACAAG TGCGGACAAA 
AACTTCAGCA AAAGCACACT GATGCGCTCC GGATCCATCG AGAGACCAGC TAAAGAAGTC 
CCAGGAGGCC CACAAAACAC CACTGGTCAA ACTCTGCCTG CGACCCACAT GCCCAGGAAA 
ACGTATTTGA AGCCGAAGCG TGTGAAGGCC ATGTATAACT GTGTGGCCGA TAATCCAGAC 
GAGCTGACCT TCTCTGAGGG AGAGCTTATC GTGGTGGATG GAGAGGAGGA CCAGGAGTGG 
TGGCTGGGCC ACATTGAGGG AGAGCCAATG AGAAGAGGAG CGTTTCCTGT CACGTTTGTA 
CAGTTCATTA TGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207157.1
CDS	428..3385
Translation	MPDQITVAEFVTETNEDYKSPTASNFTTRMTHCRNTVSALEEALDVDRSVLYKMKKSVKAIYASGLAHVENEEQYTQALEKFGENCVYRDDPDLGSAFLKFSVFTKELTALFKNLFQNMNNIITFPLDSLLKGDLKGVKGDLKKPFDKAWKDYETKVSKIEKEKKEHARQHGMIRTEISGAEIAEEMEKERRFFQLQMCEYLLKVNEIKIKKGVDLLQNLIKYFHAQCNFFQDGLKAVDNLKPSIEKLATDLHSIKQVQDEERRQLTQLRDVLKTALQVEQKESRRDSQVRQSATYSLHQPQGNKEHGTERSGNLYKKSDGLRKVWQKRKCTVKNGYLTISHGTANRPPAKLNLLTCQVKHNPEEKKSFDLISHDRTYHFQAEDEPECQIWISVLQNSKEEALNNAFKGDQHVGENNIVQELTKAILGEVKRMAGNDVCCDCGAPGPTWLSTNLGILTCIECSGIHRELGVHYSRIQSLTLDVLSTSELLLAKNVGNAGFNEIMEACLTAEDVIKPNPASDMQARKDFIMAKYTEKRFARKKCPDALSKLHTLCDAVKARDIFSLIQVYAEGVDLMEPIPLANGHEQGETALHLAVRLVDRTSLHIIDFLTQNSLNLDKQTAKGSTALHYCCLTDNSECLKLLLRGKASIDIANEAGETPLDIARRLKHLQCEELLNQALAGKFNAHVHVEYEWRLQHEDLDESDEDLDEKSSPHRRDERPISCYTPGSNSLQLSPASLSRDGRDLVKDKQRFVPNLVNNETYGTIINTSSPVSLSSSAPPLPPRNLVQPSALAGLTQGSPGWKPGSLDLSGRQRSSSDPPNMHPPAPPLRVTSTSLLMPSGAAPPLAKATGMMETMNMQPKPGQGPPGQNINRATSADKNFSKSTLMRSGSIERPAKEVPGGPQNTTGQTLPATHMPRKTYLKPKRVKAMYNCVADNPDELTFSEGELIVVDGEEDQEWWLGHIEGEPMRRGAFPVTFVQFIMD

Target ORF information:

RefSeq Version	XM_017351668.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2a (asap2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351668.1

ORF Insert Sequence:

ATGCCTGACC AGATAACAGT GGCGGAGTTT GTCACGGAGA CAAATGAAGA TTATAAATCG 
CCCACCGCCT CAAACTTCAC CACCAGAATG ACTCACTGCA GGAACACAGT ATCCGCACTG 
GAGGAGGCCC TGGATGTGGA CCGCAGTGTC CTTTACAAGA TGAAGAAGTC AGTTAAGGCT 
ATTTACGCCT CGGGTCTGGC TCATGTGGAG AATGAGGAGC AGTACACTCA AGCTCTGGAG 
AAGTTCGGAG AGAACTGTGT GTACAGAGAT GACCCGGACC TGGGATCAGC CTTCCTGAAG 
TTCTCCGTCT TCACCAAGGA GCTCACGGCA CTCTTCAAGA ACCTGTTTCA GAACATGAAT 
AATATCATTA CCTTCCCATT GGACAGTCTG CTGAAGGGAG ATCTGAAAGG GGTTAAAGGG 
GATCTCAAGA AGCCCTTCGA TAAAGCCTGG AAAGACTACG AGACTAAAGT CTCTAAAATA 
GAGAAGGAGA AAAAAGAGCA CGCCCGGCAG CACGGAATGA TCCGGACGGA GATCAGCGGA 
GCAGAGATAG CAGAAGAGAT GGAAAAAGAG CGGCGTTTCT TCCAGCTTCA GATGTGTGAG 
TACCTCCTCA AAGTCAATGA AATCAAGATC AAAAAAGGTG TCGACCTGCT CCAGAATCTC 
ATCAAATACT TCCACGCACA GTGCAACTTC TTTCAGGATG GTCTCAAAGC GGTGGACAAC 
CTCAAACCCT CAATAGAAAA ACTGGCCACA GACTTGCACT CGATCAAACA GGTACAGGAT 
GAAGAACGCA GACAGCTAAC CCAGTTACGG GATGTGCTAA AAACTGCTCT GCAAGTGGAG 
CAGAAGGAGT CTAGGAGAGA CTCTCAGGTT AGACAGAGCG CCACCTACAG TCTGCACCAG 
CCGCAGGGCA ACAAAGAGCA TGGGACTGAG CGCAGCGGCA ACCTTTACAA GAAGAGTGAC 
GGGCTGCGGA AAGTGTGGCA GAAGAGAAAG TGCACAGTAA AGAATGGATA TTTGACCATC 
TCACATGGGA CGGCAAACAG ACCTCCCGCC AAACTCAATC TTCTCACCTG TCAGGTGAAG 
CACAACCCAG AGGAGAAGAA AAGTTTTGAC CTCATCTCAC ATGACAGAAC ATATCATTTC 
CAGGCAGAAG ATGAGCCAGA GTGTCAAATA TGGATCTCAG TGCTGCAGAA CAGTAAAGAA 
GAGGCGCTCA ACAACGCCTT CAAGGGCGAC CAGCATGTTG GTGAAAATAA CATTGTGCAG 
GAGCTCACCA AGGCCATCCT GGGAGAGGTG AAGCGGATGG CGGGGAACGA TGTCTGCTGC 
GACTGCGGTG CTCCCGGCCC CACATGGCTC TCCACCAACC TGGGCATCCT GACCTGCATC 
GAGTGTTCGG GGATCCACAG AGAGCTGGGC GTCCATTACT CCCGAATCCA GTCCCTCACA 
CTCGACGTCC TCAGCACCTC CGAGCTCTTG CTGGCCAAGA ACGTGGGGAA TGCTGGCTTC 
AATGAGATCA TGGAGGCCTG TCTGACGGCA GAAGATGTGA TCAAACCGAA TCCAGCCAGT 
GACATGCAGG CGAGGAAGGA CTTTATCATG GCCAAATACA CAGAGAAACG CTTCGCTCGT 
AAGAAGTGTC CAGACGCACT GTCGAAGCTG CACACGCTCT GTGATGCTGT GAAGGCCCGG 
GACATTTTCT CTCTCATCCA GGTCTATGCT GAAGGAGTGG ATCTGATGGA GCCCATTCCT 
CTGGCTAATG GACATGAACA AGGTGAGACG GCTCTTCATC TGGCCGTGAG ACTGGTGGAC 
AGAACTTCCC TACACATCAT CGACTTCCTC ACCCAAAACA GTTTAAACCT GGATAAGCAA 
ACGGCTAAAG GAAGCACAGC TCTGCATTAC TGCTGCCTGA CGGACAACAG CGAGTGTCTC 
AAACTGCTGC TCAGAGGAAA AGCCTCCATA GATATCGCTA ATGAAGCTGG AGAGACCCCG 
TTGGACATCG CCAGGCGACT CAAACATCTG CAGTGTGAGG AACTGCTGAA CCAGGCTCTT 
GCAGGGAAGT TCAATGCTCA TGTGCATGTG GAGTATGAGT GGAGACTTCA GCATGAAGAC 
CTGGACGAGA GTGATGAAGA TCTGGATGAG AAGTCGAGTC CTCACCGGCG GGATGAGCGG 
CCCATCAGCT GCTACACACC GGGCAGTAAC TCCCTTCAGC TGAGTCCAGC CAGCCTGAGC 
CGAGACGGTC GAGACCTGGT TAAAGACAAG CAACGCTTTG TGCCAAACCT GGTCAACAAT 
GAAACCTACG GGACCATCAT TAACACCAGC TCACCCGTCA GCCTGTCCTC TTCTGCTCCA 
CCTCTACCAC CCCGAAACCT AGTTCAGCCG TCTGCTCTTG CAGGACTGAC TCAAGGATCT 
CCCGGCTGGA AGCCTGGCTC TCTGGATCTG AGCGGCAGAC AGAGATCCTC CTCTGACCCT 
CCCAACATGC ATCCTCCTGC GCCTCCCTTA CGGGTCACTT CCACCTCCCT TCTAATGCCC 
AGCGGTGCTG CTCCTCCTCT GGCTAAAGCT ACTGGTATGA TGGAGACCAT GAATATGCAA 
CCCAAACCCG GACAGGGGCC TCCTGGACAG AACATCAACC GGGCTACAAG TGCGGACAAA 
AACTTCAGCA AAAGCACACT GATGCGCTCC GGATCCATCG AGAGACCAGC TAAAGAAGTC 
CCAGGAGGCC CACAAAACAC CACTGGTCAA ACTCTGCCTG CGACCCACAT GCCCAGGAAA 
ACGTATTTGA AGCCGAAGCG TGTGAAGGCC ATGTATAACT GTGTGGCCGA TAATCCAGAC 
GAGCTGACCT TCTCTGAGGG AGAGCTTATC GTGGTGGATG GAGAGGAGGA CCAGGAGTGG 
TGGCTGGGCC ACATTGAGGG AGAGCCAATG AGAAGAGGAG CGTTTCCTGT CACGTTTGTA 
CAGTTCATTA TGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap2a ( XM_021467218.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322893.1 Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2a (asap2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa149578
Clone ID Related Accession (Same CDS sequence)	XM_021467218.1
Accession Version	XM_021467218.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3003bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGACC AGATAACAGT GGCGGAGTTT GTCACGGAGA CAAATGAAGA TTATAAATCG 
CCCACCGCCT CAAACTTCAC CACCAGAATG ACTCACTGCA GGAACACAGT ATCCGCACTG 
GAGGAGGCCC TGGATGTGGA CCGCAGTGTC CTTTACAAGA TGAAGAAGTC AGTTAAGGCT 
ATTTACGCCT CGGGTCTGGC TCATGTGGAG AATGAGGAGC AGTACACTCA AGCTCTGGAG 
AAGTTCGGAG AGAACTGTGT GTACAGAGAT GACCCGGACC TGGGATCAGC CTTCCTGAAG 
TTCTCCGTCT TCACCAAGGA GCTCACGGCA CTCTTCAAGA ACCTGTTTCA GAACATGAAT 
AATATCATTA CCTTCCCATT GGACAGTCTG CTGAAGGGAG ATCTGAAAGG GGTTAAAGGG 
GATCTCAAGA AGCCCTTCGA TAAAGCCTGG AAAGACTACG AGACTAAAGT CTGCAAGGGA 
CATTCTTTAC TGAAAGGCTC TAAAATAGAG AAGGAGAAAA AAGAGCACGC CCGGCAGCAC 
GGAATGATCC GGACGGAGAT CAGCGGAGCA GAGATAGCAG AAGAGATGGA AAAAGAGCGG 
CGTTTCTTCC AGCTTCAGAT GTGTGAGTAC CTCCTCAAAG TCAATGAAAT CAAGATCAAA 
AAAGGTGTCG ACCTGCTCCA GAATCTCATC AAATACTTCC ACGCACAGTG CAACTTCTTT 
CAGGATGGTC TCAAAGCGGT GGACAACCTC AAACCCTCAA TAGAAAAACT GGCCACAGAC 
TTGCACTCGA TCAAACAGGT ACAGGATGAA GAACGCAGAC AGCTAACCCA GTTACGGGAT 
GTGCTAAAAA CTGCTCTGCA AGTGGAGCAG AAGGAGGACT CTCAGGTTAG ACAGAGCGCC 
ACCTACAGTC TGCACCAGCC GCAGGGCAAC AAAGAGCATG GGACTGAGCG CAGCGGCAAC 
CTTTACAAGA AGAGTGACGG GCTGCGGAAA GTGTGGCAGA AGAGAAAGTG CACAGTAAAG 
AATGGATATT TGACCATCTC ACATGGGACG GCAAACAGAC CTCCCGCCAA ACTCAATCTT 
CTCACCTGTC AGGTGAAGCA CAACCCAGAG GAGAAGAAAA GTTTTGACCT CATCTCACAT 
GACAGAACAT ATCATTTCCA GGCAGAAGAT GAGCCAGAGT GTCAAATATG GATCTCAGTG 
CTGCAGAACA GTAAAGAAGA GGCGCTCAAC AACGCCTTCA AGGGCGACCA GCATGTTGGT 
GAAAATAACA TTGTGCAGGA GCTCACCAAG GCCATCCTGG GAGAGGTGAA GCGGATGGCG 
GGGAACGATG TCTGCTGCGA CTGCGGTGCT CCCGGCCCCA CATGGCTCTC CACCAACCTG 
GGCATCCTGA CCTGCATCGA GTGTTCGGGG ATCCACAGAG AGCTGGGCGT CCATTACTCC 
CGAATCCAGT CCCTCACACT CGACGTCCTC AGCACCTCCG AGCTCTTGCT GGCCAAGAAC 
GTGGGGAATG CTGGCTTCAA TGAGATCATG GAGGCCTGTC TGACGGCAGA AGATGTGATC 
AAACCGAATC CAGCCAGTGA CATGCAGGCG AGGAAGGACT TTATCATGGC CAAATACACA 
GAGAAACGCT TCGCTCGTAA GAAGTGTCCA GACGCACTGT CGAAGCTGCA CACGCTCTGT 
GATGCTGTGA AGGCCCGGGA CATTTTCTCT CTCATCCAGG TCTATGCTGA AGGAGTGGAT 
CTGATGGAGC CCATTCCTCT GGCTAATGGA CATGAACAAG GTGAGACGGC TCTTCATCTG 
GCCGTGAGAC TGGTGGACAG AACTTCCCTA CACATCATCG ACTTCCTCAC CCAAAACAGT 
TTAAACCTGG ATAAGCAAAC GGCTAAAGGA AGCACAGCTC TGCATTACTG CTGCCTGACG 
GACAACAGCG AGTGTCTCAA ACTGCTGCTC AGAGGAAAAG CCTCCATAGA TATCGCTAAT 
GAAGCTGGAG AGACCCCGTT GGACATCGCC AGGCGACTCA AACATCTGCA GTGTGAGGAA 
CTGCTGAACC AGGCTCTTGC AGGGAAGTTC AATGCTCATG TGCATGTGGA GTATGAGTGG 
AGACTTCAGC ATGAAGACCT GGACGAGAGT GATGAAGATC TGGATGAGAA GTCGAGTCCT 
CACCGGCGGG ATGAGCGGCC CATCAGCTGC TACACACCGG GCAGTAACTC CCTTCAGCTG 
AGTCCAGCCA GCCTGAGCCG AGACGGTCGA GACCTGGTTA AAGACAAGCA ACGCTTTGTG 
CCAAACCTGG TCAACAATGA AACCTACGGG ACCATCATTA ACACCAGCTC ACCCGTCAGC 
CTGTCCTCTT CTGCTCCACC TCTACCACCC CGAAACCTAG TTCAGCCGTC TGCTCTTGCA 
GGACTGACTC AAGGATCTCC CGGCTGGAAG CCTGGCTCTC TGGATCTGAG CGGCAGACAG 
AGATCCTCCT CTGACCCTCC CAACATGCAT CCTCCTGCGC CTCCCTTACG GGTCACTTCC 
ACCTCCCTTC TAATGCCCAG CGGTGCTGCT CCTCCTCTGG CTAAAGCTAC TGGTATGATG 
GAGACCATGA ATATGCAACC CAAACCCGGA CAGGGGCCTC CTGGACAGAA CATCAACCGG 
GCTACAAGTG CGGACAAAAA CTTCAGCAAA AGCACACTGA TGCGCTCCGG ATCCATCGAG 
AGACCAGGTA GATGTCAGAG AAACCATGTT AACTCTAAAG AAGTCCCAGG AGGCCCACAA 
AACACCACTG GTCAAACTCT GCCTGCGACC CACATGCCCA GGAAAACGTA TTTGAAGCCG 
AAGCGTGTGA AGGCCATGTA TAACTGTGTG GCCGATAATC CAGACGAGCT GACCTTCTCT 
GAGGGAGAGC TTATCGTGGT GGATGGAGAG GAGGACCAGG AGTGGTGGCT GGGCCACATT 
GAGGGAGAGC CAATGAGAAG AGGAGCGTTT CCTGTCACGT TTGTACAGTT CATTATGGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322893.1
CDS	440..3442
Translation	MPDQITVAEFVTETNEDYKSPTASNFTTRMTHCRNTVSALEEALDVDRSVLYKMKKSVKAIYASGLAHVENEEQYTQALEKFGENCVYRDDPDLGSAFLKFSVFTKELTALFKNLFQNMNNIITFPLDSLLKGDLKGVKGDLKKPFDKAWKDYETKVCKGHSLLKGSKIEKEKKEHARQHGMIRTEISGAEIAEEMEKERRFFQLQMCEYLLKVNEIKIKKGVDLLQNLIKYFHAQCNFFQDGLKAVDNLKPSIEKLATDLHSIKQVQDEERRQLTQLRDVLKTALQVEQKEDSQVRQSATYSLHQPQGNKEHGTERSGNLYKKSDGLRKVWQKRKCTVKNGYLTISHGTANRPPAKLNLLTCQVKHNPEEKKSFDLISHDRTYHFQAEDEPECQIWISVLQNSKEEALNNAFKGDQHVGENNIVQELTKAILGEVKRMAGNDVCCDCGAPGPTWLSTNLGILTCIECSGIHRELGVHYSRIQSLTLDVLSTSELLLAKNVGNAGFNEIMEACLTAEDVIKPNPASDMQARKDFIMAKYTEKRFARKKCPDALSKLHTLCDAVKARDIFSLIQVYAEGVDLMEPIPLANGHEQGETALHLAVRLVDRTSLHIIDFLTQNSLNLDKQTAKGSTALHYCCLTDNSECLKLLLRGKASIDIANEAGETPLDIARRLKHLQCEELLNQALAGKFNAHVHVEYEWRLQHEDLDESDEDLDEKSSPHRRDERPISCYTPGSNSLQLSPASLSRDGRDLVKDKQRFVPNLVNNETYGTIINTSSPVSLSSSAPPLPPRNLVQPSALAGLTQGSPGWKPGSLDLSGRQRSSSDPPNMHPPAPPLRVTSTSLLMPSGAAPPLAKATGMMETMNMQPKPGQGPPGQNINRATSADKNFSKSTLMRSGSIERPGRCQRNHVNSKEVPGGPQNTTGQTLPATHMPRKTYLKPKRVKAMYNCVADNPDELTFSEGELIVVDGEEDQEWWLGHIEGEPMRRGAFPVTFVQFIMD

Target ORF information:

RefSeq Version	XM_021467218.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2a (asap2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467218.1

ORF Insert Sequence:

ATGCCTGACC AGATAACAGT GGCGGAGTTT GTCACGGAGA CAAATGAAGA TTATAAATCG 
CCCACCGCCT CAAACTTCAC CACCAGAATG ACTCACTGCA GGAACACAGT ATCCGCACTG 
GAGGAGGCCC TGGATGTGGA CCGCAGTGTC CTTTACAAGA TGAAGAAGTC AGTTAAGGCT 
ATTTACGCCT CGGGTCTGGC TCATGTGGAG AATGAGGAGC AGTACACTCA AGCTCTGGAG 
AAGTTCGGAG AGAACTGTGT GTACAGAGAT GACCCGGACC TGGGATCAGC CTTCCTGAAG 
TTCTCCGTCT TCACCAAGGA GCTCACGGCA CTCTTCAAGA ACCTGTTTCA GAACATGAAT 
AATATCATTA CCTTCCCATT GGACAGTCTG CTGAAGGGAG ATCTGAAAGG GGTTAAAGGG 
GATCTCAAGA AGCCCTTCGA TAAAGCCTGG AAAGACTACG AGACTAAAGT CTGCAAGGGA 
CATTCTTTAC TGAAAGGCTC TAAAATAGAG AAGGAGAAAA AAGAGCACGC CCGGCAGCAC 
GGAATGATCC GGACGGAGAT CAGCGGAGCA GAGATAGCAG AAGAGATGGA AAAAGAGCGG 
CGTTTCTTCC AGCTTCAGAT GTGTGAGTAC CTCCTCAAAG TCAATGAAAT CAAGATCAAA 
AAAGGTGTCG ACCTGCTCCA GAATCTCATC AAATACTTCC ACGCACAGTG CAACTTCTTT 
CAGGATGGTC TCAAAGCGGT GGACAACCTC AAACCCTCAA TAGAAAAACT GGCCACAGAC 
TTGCACTCGA TCAAACAGGT ACAGGATGAA GAACGCAGAC AGCTAACCCA GTTACGGGAT 
GTGCTAAAAA CTGCTCTGCA AGTGGAGCAG AAGGAGGACT CTCAGGTTAG ACAGAGCGCC 
ACCTACAGTC TGCACCAGCC GCAGGGCAAC AAAGAGCATG GGACTGAGCG CAGCGGCAAC 
CTTTACAAGA AGAGTGACGG GCTGCGGAAA GTGTGGCAGA AGAGAAAGTG CACAGTAAAG 
AATGGATATT TGACCATCTC ACATGGGACG GCAAACAGAC CTCCCGCCAA ACTCAATCTT 
CTCACCTGTC AGGTGAAGCA CAACCCAGAG GAGAAGAAAA GTTTTGACCT CATCTCACAT 
GACAGAACAT ATCATTTCCA GGCAGAAGAT GAGCCAGAGT GTCAAATATG GATCTCAGTG 
CTGCAGAACA GTAAAGAAGA GGCGCTCAAC AACGCCTTCA AGGGCGACCA GCATGTTGGT 
GAAAATAACA TTGTGCAGGA GCTCACCAAG GCCATCCTGG GAGAGGTGAA GCGGATGGCG 
GGGAACGATG TCTGCTGCGA CTGCGGTGCT CCCGGCCCCA CATGGCTCTC CACCAACCTG 
GGCATCCTGA CCTGCATCGA GTGTTCGGGG ATCCACAGAG AGCTGGGCGT CCATTACTCC 
CGAATCCAGT CCCTCACACT CGACGTCCTC AGCACCTCCG AGCTCTTGCT GGCCAAGAAC 
GTGGGGAATG CTGGCTTCAA TGAGATCATG GAGGCCTGTC TGACGGCAGA AGATGTGATC 
AAACCGAATC CAGCCAGTGA CATGCAGGCG AGGAAGGACT TTATCATGGC CAAATACACA 
GAGAAACGCT TCGCTCGTAA GAAGTGTCCA GACGCACTGT CGAAGCTGCA CACGCTCTGT 
GATGCTGTGA AGGCCCGGGA CATTTTCTCT CTCATCCAGG TCTATGCTGA AGGAGTGGAT 
CTGATGGAGC CCATTCCTCT GGCTAATGGA CATGAACAAG GTGAGACGGC TCTTCATCTG 
GCCGTGAGAC TGGTGGACAG AACTTCCCTA CACATCATCG ACTTCCTCAC CCAAAACAGT 
TTAAACCTGG ATAAGCAAAC GGCTAAAGGA AGCACAGCTC TGCATTACTG CTGCCTGACG 
GACAACAGCG AGTGTCTCAA ACTGCTGCTC AGAGGAAAAG CCTCCATAGA TATCGCTAAT 
GAAGCTGGAG AGACCCCGTT GGACATCGCC AGGCGACTCA AACATCTGCA GTGTGAGGAA 
CTGCTGAACC AGGCTCTTGC AGGGAAGTTC AATGCTCATG TGCATGTGGA GTATGAGTGG 
AGACTTCAGC ATGAAGACCT GGACGAGAGT GATGAAGATC TGGATGAGAA GTCGAGTCCT 
CACCGGCGGG ATGAGCGGCC CATCAGCTGC TACACACCGG GCAGTAACTC CCTTCAGCTG 
AGTCCAGCCA GCCTGAGCCG AGACGGTCGA GACCTGGTTA AAGACAAGCA ACGCTTTGTG 
CCAAACCTGG TCAACAATGA AACCTACGGG ACCATCATTA ACACCAGCTC ACCCGTCAGC 
CTGTCCTCTT CTGCTCCACC TCTACCACCC CGAAACCTAG TTCAGCCGTC TGCTCTTGCA 
GGACTGACTC AAGGATCTCC CGGCTGGAAG CCTGGCTCTC TGGATCTGAG CGGCAGACAG 
AGATCCTCCT CTGACCCTCC CAACATGCAT CCTCCTGCGC CTCCCTTACG GGTCACTTCC 
ACCTCCCTTC TAATGCCCAG CGGTGCTGCT CCTCCTCTGG CTAAAGCTAC TGGTATGATG 
GAGACCATGA ATATGCAACC CAAACCCGGA CAGGGGCCTC CTGGACAGAA CATCAACCGG 
GCTACAAGTG CGGACAAAAA CTTCAGCAAA AGCACACTGA TGCGCTCCGG ATCCATCGAG 
AGACCAGGTA GATGTCAGAG AAACCATGTT AACTCTAAAG AAGTCCCAGG AGGCCCACAA 
AACACCACTG GTCAAACTCT GCCTGCGACC CACATGCCCA GGAAAACGTA TTTGAAGCCG 
AAGCGTGTGA AGGCCATGTA TAACTGTGTG GCCGATAATC CAGACGAGCT GACCTTCTCT 
GAGGGAGAGC TTATCGTGGT GGATGGAGAG GAGGACCAGG AGTGGTGGCT GGGCCACATT 
GAGGGAGAGC CAATGAGAAG AGGAGCGTTT CCTGTCACGT TTGTACAGTT CATTATGGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

asap2a ( XM_021467217.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322892.1 Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2a (asap2a), transcript variant X1, mRNA.

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CloneID	ODa46006
Clone ID Related Accession (Same CDS sequence)	XM_021467217.1
Accession Version	XM_021467217.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGACC AGATAACAGT GGCGGAGTTT GTCACGGAGA CAAATGAAGA TTATAAATCG 
CCCACCGCCT CAAACTTCAC CACCAGAATG ACTCACTGCA GGAACACAGT ATCCGCACTG 
GAGGAGGCCC TGGATGTGGA CCGCAGTGTC CTTTACAAGA TGAAGAAGTC AGTTAAGGCT 
ATTTACGCCT CGGGTCTGGC TCATGTGGAG AATGAGGAGC AGTACACTCA AGCTCTGGAG 
AAGTTCGGAG AGAACTGTGT GTACAGAGAT GACCCGGACC TGGGATCAGC CTTCCTGAAG 
TTCTCCGTCT TCACCAAGGA GCTCACGGCA CTCTTCAAGA ACCTGTTTCA GAACATGAAT 
AATATCATTA CCTTCCCATT GGACAGTCTG CTGAAGGGAG ATCTGAAAGG GGTTAAAGGG 
GATCTCAAGA AGCCCTTCGA TAAAGCCTGG AAAGACTACG AGACTAAAGT CTGCAAGGGA 
CATTCTTTAC TGAAAGGCTC TAAAATAGAG AAGGAGAAAA AAGAGCACGC CCGGCAGCAC 
GGAATGATCC GGACGGAGAT CAGCGGAGCA GAGATAGCAG AAGAGATGGA AAAAGAGCGG 
CGTTTCTTCC AGCTTCAGAT GTGTGAGTAC CTCCTCAAAG TCAATGAAAT CAAGATCAAA 
AAAGGTGTCG ACCTGCTCCA GAATCTCATC AAATACTTCC ACGCACAGTG CAACTTCTTT 
CAGGATGGTC TCAAAGCGGT GGACAACCTC AAACCCTCAA TAGAAAAACT GGCCACAGAC 
TTGCACTCGA TCAAACAGGT ACAGGATGAA GAACGCAGAC AGCTAACCCA GTTACGGGAT 
GTGCTAAAAA CTGCTCTGCA AGTGGAGCAG AAGGAGTCTA GGAGAGACTC TCAGGTTAGA 
CAGAGCGCCA CCTACAGTCT GCACCAGCCG CAGGGCAACA AAGAGCATGG GACTGAGCGC 
AGCGGCAACC TTTACAAGAA GAGTGACGGG CTGCGGAAAG TGTGGCAGAA GAGAAAGTGC 
ACAGTAAAGA ATGGATATTT GACCATCTCA CATGGGACGG CAAACAGACC TCCCGCCAAA 
CTCAATCTTC TCACCTGTCA GGTGAAGCAC AACCCAGAGG AGAAGAAAAG TTTTGACCTC 
ATCTCACATG ACAGAACATA TCATTTCCAG GCAGAAGATG AGCCAGAGTG TCAAATATGG 
ATCTCAGTGC TGCAGAACAG TAAAGAAGAG GCGCTCAACA ACGCCTTCAA GGGCGACCAG 
CATGTTGGTG AAAATAACAT TGTGCAGGAG CTCACCAAGG CCATCCTGGG AGAGGTGAAG 
CGGATGGCGG GGAACGATGT CTGCTGCGAC TGCGGTGCTC CCGGCCCCAC ATGGCTCTCC 
ACCAACCTGG GCATCCTGAC CTGCATCGAG TGTTCGGGGA TCCACAGAGA GCTGGGCGTC 
CATTACTCCC GAATCCAGTC CCTCACACTC GACGTCCTCA GCACCTCCGA GCTCTTGCTG 
GCCAAGAACG TGGGGAATGC TGGCTTCAAT GAGATCATGG AGGCCTGTCT GACGGCAGAA 
GATGTGATCA AACCGAATCC AGCCAGTGAC ATGCAGGCGA GGAAGGACTT TATCATGGCC 
AAATACACAG AGAAACGCTT CGCTCGTAAG AAGTGTCCAG ACGCACTGTC GAAGCTGCAC 
ACGCTCTGTG ATGCTGTGAA GGCCCGGGAC ATTTTCTCTC TCATCCAGGT CTATGCTGAA 
GGAGTGGATC TGATGGAGCC CATTCCTCTG GCTAATGGAC ATGAACAAGG TGAGACGGCT 
CTTCATCTGG CCGTGAGACT GGTGGACAGA ACTTCCCTAC ACATCATCGA CTTCCTCACC 
CAAAACAGTT TAAACCTGGA TAAGCAAACG GCTAAAGGAA GCACAGCTCT GCATTACTGC 
TGCCTGACGG ACAACAGCGA GTGTCTCAAA CTGCTGCTCA GAGGAAAAGC CTCCATAGAT 
ATCGCTAATG AAGCTGGAGA GACCCCGTTG GACATCGCCA GGCGACTCAA ACATCTGCAG 
TGTGAGGAAC TGCTGAACCA GGCTCTTGCA GGGAAGTTCA ATGCTCATGT GCATGTGGAG 
TATGAGTGGA GACTTCAGCA TGAAGACCTG GACGAGAGTG ATGAAGATCT GGATGAGAAG 
TCGAGTCCTC ACCGGCGGGA TGAGCGGCCC ATCAGCTGCT ACACACCGGG CAGTAACTCC 
CTTCAGCTGA GTCCAGCCAG CCTGAGCCGA GACGGTCGAG ACCTGGTTAA AGACAAGCAA 
CGCTTTGTGC CAAACCTGGT CAACAATGAA ACCTACGGGA CCATCATTAA CACCAGCTCA 
CCCGTCAGCC TGTCCTCTTC TGCTCCACCT CTACCACCCC GAAACCTAGT TCAGCCGTCT 
GCTCTTGCAG GACTGACTCA AGGATCTCCC GGCTGGAAGC CTGGCTCTCT GGATCTGAGC 
GGCAGACAGA GATCCTCCTC TGACCCTCCC AACATGCATC CTCCTGCGCC TCCCTTACGG 
GTCACTTCCA CCTCCCTTCT AATGCCCAGC GGTGCTGCTC CTCCTCTGGC TAAAGCTACT 
GGTATGATGG AGACCATGAA TATGCAACCC AAACCCGGAC AGGGGCCTCC TGGACAGAAC 
ATCAACCGGG CTACAAGTGC GGACAAAAAC TTCAGCAAAA GCACACTGAT GCGCTCCGGA 
TCCATCGAGA GACCAGGTAG ATGTCAGAGA AACCATGTTA ACTCTAAAGA AGTCCCAGGA 
GGCCCACAAA ACACCACTGG TCAAACTCTG CCTGCGACCC ACATGCCCAG GAAAACGTAT 
TTGAAGCCGA AGCGTGTGAA GGCCATGTAT AACTGTGTGG CCGATAATCC AGACGAGCTG 
ACCTTCTCTG AGGGAGAGCT TATCGTGGTG GATGGAGAGG AGGACCAGGA GTGGTGGCTG 
GGCCACATTG AGGGAGAGCC AATGAGAAGA GGAGCGTTTC CTGTCACGTT TGTACAGTTC 
ATTATGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322892.1
CDS	440..3451
Translation	MPDQITVAEFVTETNEDYKSPTASNFTTRMTHCRNTVSALEEALDVDRSVLYKMKKSVKAIYASGLAHVENEEQYTQALEKFGENCVYRDDPDLGSAFLKFSVFTKELTALFKNLFQNMNNIITFPLDSLLKGDLKGVKGDLKKPFDKAWKDYETKVCKGHSLLKGSKIEKEKKEHARQHGMIRTEISGAEIAEEMEKERRFFQLQMCEYLLKVNEIKIKKGVDLLQNLIKYFHAQCNFFQDGLKAVDNLKPSIEKLATDLHSIKQVQDEERRQLTQLRDVLKTALQVEQKESRRDSQVRQSATYSLHQPQGNKEHGTERSGNLYKKSDGLRKVWQKRKCTVKNGYLTISHGTANRPPAKLNLLTCQVKHNPEEKKSFDLISHDRTYHFQAEDEPECQIWISVLQNSKEEALNNAFKGDQHVGENNIVQELTKAILGEVKRMAGNDVCCDCGAPGPTWLSTNLGILTCIECSGIHRELGVHYSRIQSLTLDVLSTSELLLAKNVGNAGFNEIMEACLTAEDVIKPNPASDMQARKDFIMAKYTEKRFARKKCPDALSKLHTLCDAVKARDIFSLIQVYAEGVDLMEPIPLANGHEQGETALHLAVRLVDRTSLHIIDFLTQNSLNLDKQTAKGSTALHYCCLTDNSECLKLLLRGKASIDIANEAGETPLDIARRLKHLQCEELLNQALAGKFNAHVHVEYEWRLQHEDLDESDEDLDEKSSPHRRDERPISCYTPGSNSLQLSPASLSRDGRDLVKDKQRFVPNLVNNETYGTIINTSSPVSLSSSAPPLPPRNLVQPSALAGLTQGSPGWKPGSLDLSGRQRSSSDPPNMHPPAPPLRVTSTSLLMPSGAAPPLAKATGMMETMNMQPKPGQGPPGQNINRATSADKNFSKSTLMRSGSIERPGRCQRNHVNSKEVPGGPQNTTGQTLPATHMPRKTYLKPKRVKAMYNCVADNPDELTFSEGELIVVDGEEDQEWWLGHIEGEPMRRGAFPVTFVQFIMD

Target ORF information:

RefSeq Version	XM_021467217.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ArfGAP with SH3 domain, ankyrin repeat and PH domain 2a (asap2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467217.1

ORF Insert Sequence:

ATGCCTGACC AGATAACAGT GGCGGAGTTT GTCACGGAGA CAAATGAAGA TTATAAATCG 
CCCACCGCCT CAAACTTCAC CACCAGAATG ACTCACTGCA GGAACACAGT ATCCGCACTG 
GAGGAGGCCC TGGATGTGGA CCGCAGTGTC CTTTACAAGA TGAAGAAGTC AGTTAAGGCT 
ATTTACGCCT CGGGTCTGGC TCATGTGGAG AATGAGGAGC AGTACACTCA AGCTCTGGAG 
AAGTTCGGAG AGAACTGTGT GTACAGAGAT GACCCGGACC TGGGATCAGC CTTCCTGAAG 
TTCTCCGTCT TCACCAAGGA GCTCACGGCA CTCTTCAAGA ACCTGTTTCA GAACATGAAT 
AATATCATTA CCTTCCCATT GGACAGTCTG CTGAAGGGAG ATCTGAAAGG GGTTAAAGGG 
GATCTCAAGA AGCCCTTCGA TAAAGCCTGG AAAGACTACG AGACTAAAGT CTGCAAGGGA 
CATTCTTTAC TGAAAGGCTC TAAAATAGAG AAGGAGAAAA AAGAGCACGC CCGGCAGCAC 
GGAATGATCC GGACGGAGAT CAGCGGAGCA GAGATAGCAG AAGAGATGGA AAAAGAGCGG 
CGTTTCTTCC AGCTTCAGAT GTGTGAGTAC CTCCTCAAAG TCAATGAAAT CAAGATCAAA 
AAAGGTGTCG ACCTGCTCCA GAATCTCATC AAATACTTCC ACGCACAGTG CAACTTCTTT 
CAGGATGGTC TCAAAGCGGT GGACAACCTC AAACCCTCAA TAGAAAAACT GGCCACAGAC 
TTGCACTCGA TCAAACAGGT ACAGGATGAA GAACGCAGAC AGCTAACCCA GTTACGGGAT 
GTGCTAAAAA CTGCTCTGCA AGTGGAGCAG AAGGAGTCTA GGAGAGACTC TCAGGTTAGA 
CAGAGCGCCA CCTACAGTCT GCACCAGCCG CAGGGCAACA AAGAGCATGG GACTGAGCGC 
AGCGGCAACC TTTACAAGAA GAGTGACGGG CTGCGGAAAG TGTGGCAGAA GAGAAAGTGC 
ACAGTAAAGA ATGGATATTT GACCATCTCA CATGGGACGG CAAACAGACC TCCCGCCAAA 
CTCAATCTTC TCACCTGTCA GGTGAAGCAC AACCCAGAGG AGAAGAAAAG TTTTGACCTC 
ATCTCACATG ACAGAACATA TCATTTCCAG GCAGAAGATG AGCCAGAGTG TCAAATATGG 
ATCTCAGTGC TGCAGAACAG TAAAGAAGAG GCGCTCAACA ACGCCTTCAA GGGCGACCAG 
CATGTTGGTG AAAATAACAT TGTGCAGGAG CTCACCAAGG CCATCCTGGG AGAGGTGAAG 
CGGATGGCGG GGAACGATGT CTGCTGCGAC TGCGGTGCTC CCGGCCCCAC ATGGCTCTCC 
ACCAACCTGG GCATCCTGAC CTGCATCGAG TGTTCGGGGA TCCACAGAGA GCTGGGCGTC 
CATTACTCCC GAATCCAGTC CCTCACACTC GACGTCCTCA GCACCTCCGA GCTCTTGCTG 
GCCAAGAACG TGGGGAATGC TGGCTTCAAT GAGATCATGG AGGCCTGTCT GACGGCAGAA 
GATGTGATCA AACCGAATCC AGCCAGTGAC ATGCAGGCGA GGAAGGACTT TATCATGGCC 
AAATACACAG AGAAACGCTT CGCTCGTAAG AAGTGTCCAG ACGCACTGTC GAAGCTGCAC 
ACGCTCTGTG ATGCTGTGAA GGCCCGGGAC ATTTTCTCTC TCATCCAGGT CTATGCTGAA 
GGAGTGGATC TGATGGAGCC CATTCCTCTG GCTAATGGAC ATGAACAAGG TGAGACGGCT 
CTTCATCTGG CCGTGAGACT GGTGGACAGA ACTTCCCTAC ACATCATCGA CTTCCTCACC 
CAAAACAGTT TAAACCTGGA TAAGCAAACG GCTAAAGGAA GCACAGCTCT GCATTACTGC 
TGCCTGACGG ACAACAGCGA GTGTCTCAAA CTGCTGCTCA GAGGAAAAGC CTCCATAGAT 
ATCGCTAATG AAGCTGGAGA GACCCCGTTG GACATCGCCA GGCGACTCAA ACATCTGCAG 
TGTGAGGAAC TGCTGAACCA GGCTCTTGCA GGGAAGTTCA ATGCTCATGT GCATGTGGAG 
TATGAGTGGA GACTTCAGCA TGAAGACCTG GACGAGAGTG ATGAAGATCT GGATGAGAAG 
TCGAGTCCTC ACCGGCGGGA TGAGCGGCCC ATCAGCTGCT ACACACCGGG CAGTAACTCC 
CTTCAGCTGA GTCCAGCCAG CCTGAGCCGA GACGGTCGAG ACCTGGTTAA AGACAAGCAA 
CGCTTTGTGC CAAACCTGGT CAACAATGAA ACCTACGGGA CCATCATTAA CACCAGCTCA 
CCCGTCAGCC TGTCCTCTTC TGCTCCACCT CTACCACCCC GAAACCTAGT TCAGCCGTCT 
GCTCTTGCAG GACTGACTCA AGGATCTCCC GGCTGGAAGC CTGGCTCTCT GGATCTGAGC 
GGCAGACAGA GATCCTCCTC TGACCCTCCC AACATGCATC CTCCTGCGCC TCCCTTACGG 
GTCACTTCCA CCTCCCTTCT AATGCCCAGC GGTGCTGCTC CTCCTCTGGC TAAAGCTACT 
GGTATGATGG AGACCATGAA TATGCAACCC AAACCCGGAC AGGGGCCTCC TGGACAGAAC 
ATCAACCGGG CTACAAGTGC GGACAAAAAC TTCAGCAAAA GCACACTGAT GCGCTCCGGA 
TCCATCGAGA GACCAGGTAG ATGTCAGAGA AACCATGTTA ACTCTAAAGA AGTCCCAGGA 
GGCCCACAAA ACACCACTGG TCAAACTCTG CCTGCGACCC ACATGCCCAG GAAAACGTAT 
TTGAAGCCGA AGCGTGTGAA GGCCATGTAT AACTGTGTGG CCGATAATCC AGACGAGCTG 
ACCTTCTCTG AGGGAGAGCT TATCGTGGTG GATGGAGAGG AGGACCAGGA GTGGTGGCTG 
GGCCACATTG AGGGAGAGCC AATGAGAAGA GGAGCGTTTC CTGTCACGTT TGTACAGTTC 
ATTATGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.
Proceedings of the National Academy of Sciences of the United States of America99(26)16899-903(2002 Dec)
Strausberg RL,Feingold EA,Grouse LH,Derge JG,Klausner RD,Collins FS,Wagner L,Shenmen CM,Schuler GD,Altschul SF,Zeeberg B,Buetow KH,Schaefer CF,Bhat NK,Hopkins RF,Jordan H,Moore T,Max SI,Wang J,Hsieh F,Diatchenko L,Marusina K,Farmer AA,Rubin GM,Hong L,Stapleton M,Soares MB,Bonaldo MF,Casavant TL,Scheetz TE,Brownstein MJ,Usdin TB,Toshiyuki S,Carninci P,Prange C,Raha SS,Loquellano NA,Peters GJ,Abramson RD,Mullahy SJ,Bosak SA,McEwan PJ,McKernan KJ,Malek JA,Gunaratne PH,Richards S,Worley KC,Hale S,Garcia AM,Gay LJ,Hulyk SW,Villalon DK,Muzny DM,Sodergren EJ,Lu X,Gibbs RA,Fahey J,Helton E,Ketteman M,Madan A,Rodrigues S,Sanchez A,Whiting M,Madan A,Young AC,Shevchenko Y,Bouffard GG,Blakesley RW,Touchman JW,Green ED,Dickson MC,Rodriguez AC,Grimwood J,Schmutz J,Myers RM,Butterfield YS,Krzywinski MI,Skalska U,Smailus DE,Schnerch A,Schein JE,Jones SJ,Marra MA,

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asap2a cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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