BBS12 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	BBS12
Entrez Gene ID	570583
Full Name	Bardet-Biedl syndrome 12
General protein information	Preferred Names Bardet-Biedl syndrome 12 Names Bardet-Biedl syndrome 12 protein
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009307657.2	XP_009305932.1	Bardet-Biedl syndrome 12 protein isoform X2
XM_021481081.1	XP_021336756.1	Bardet-Biedl syndrome 12 protein isoform X2
XM_002667206.5	XP_002667252.1	Bardet-Biedl syndrome 12 protein isoform X1
XM_002667206.4	XP_002667252.1	Bardet-Biedl syndrome 12 protein isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	BBS12	XP_004941255.1
Danio rerio (zebrafish)	bbs12	XP_002667252.1
Xenopus tropicalis (tropical clawed frog)	bbs12	XP_004911170.1
Pan troglodytes (chimpanzee)	BBS12	XP_003950450.1
Homo sapiens (human)	BBS12	NP_689831.2
	BBS12	XP_001099173.2
Canis lupus familiaris (dog)	BBS12	XP_005631891.1
Bos taurus (cattle)	BBS12	NP_001093845.1
Mus musculus (house mouse)	Bbs12	NP_001008502.2
Rattus norvegicus (Norway rat)	Bbs12	XP_006232349.1

Gene Ontology

The following BBS12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BBS12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa148782	XM_021481081.1 Latest version!	Danio rerio Bardet-Biedl syndrome 12 (bbs12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
ODa19231	XM_002667206.5 Latest version!	Danio rerio Bardet-Biedl syndrome 12 (bbs12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
ODa45989	XM_009307657.2 Latest version!	Danio rerio Bardet-Biedl syndrome 12 (bbs12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
View Old Accession Versions >>
ODa19231	XM_002667206.4	Danio rerio Bardet-Biedl syndrome 12 (bbs12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BBS12 ( XM_021481081.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021336756.1 Danio rerio Bardet-Biedl syndrome 12 (bbs12), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa148782
Clone ID Related Accession (Same CDS sequence)	XM_021481081.1
Accession Version	XM_021481081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	Bardet-Biedl syndrome 12 protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCGA GTTGTAAACA GTGCAGCTTT GCGATGAAGC GGCGTCACAT CGGCCTCCAG 
CAGCTGCAGG CCATCACATC CACCGCTCAT GCATTTCTAG GTCCAAACAA ACGGCTGAAG 
TTCATTCAAG ATGAAGACGC CGGGGATGCA GTTTTAATCG GATCCTGTCC ACGTCTGCTG 
GAACACCTGG AGCTGGACGG ATCTGTCGGA CAGCTGCTGC ACGAGACAGT TCTGGCCCAG 
AAGAAGCTCT TCCACTCGGG CACGAACACG CTGATGTTTC TGGCCGGAGC CTGGAGCCGA 
GTCGCTCTGG AGTGTCTGAA CAGAGGAATC AGTGTGTCGG ACATTAAATC AGCCATGAGG 
GGAGGACTGC AGGAGTGTTT GGATGCATGC ACACAGTTAG CGGTCAGTGT GGAGGAGTTT 
AAGAAATCTG ATGTTAGATC TAATGATCAG TGCATTGATA TGATGGTCAA ACACAGCAGA 
TACATTAATG CACGAACTAA AAACACACAA TCTGATGACA ATGTGCAAGA TTTTAGAAAG 
TTAAGTATTA AATCAGATGA TCAGTGCATG GATATGACAC ATAAACATAG CAGATATGCT 
AATACACAAA CTGAACACGC ACAATCAGAT GACAATGTGC AAGATTTTAA GGGATCAGGT 
ATTAAATCTA ATGATCAATG CATGGATATG ACACTCAAAC ACAGCAGATA CATTAATACA 
AAAACGGAAC ACGCACAATC AAATGTCAAT ATGCAAGATT TTAGGAAGTT AGATGATAAA 
TCTGATGATC AATGCAAGCA TATGACACTC AAACACAGCA GACACTTTAA TGCACGAACT 
AAAAACACAC AATCAGATGT CAATGTGCAA GATTTAAAGG GATCAGATAT TAAATCTATT 
GATCAATGCA TGGATATGAC ACTGAAACAC AGCACACACT TTAATGCACA AACTGAACAC 
AATATGCAAG ATTTTAGGAA GTTAGATGAT AAATCTGATG ATCAATGCAT GTATATGACA 
CTCAAACACA GCAGACACTT TAATGCACAA ACTGAACACA CACAATCAGA AGTTACTATG 
CAAGATTTTA CGAAATCAGA TATTAAATTG GACAATCAAA GCATGCATAT GACCCTCAAA 
CACAGCAGAC ACTTTAATGC ACAAACCGAA CACACACATC ATGATGTCAC TGTCATCGCA 
CAAGCCGTCA GCCACGGATG CTCCTCCATG CATTTACTTT TAAAAGCGTA TGAGTTGCAA 
AACCGCGCAC ATCTGGATAT ACACAATCTG GCAACGTGCT GCATCCCAGG CGTATCTGAA 
GATCATGCGT GTGTGTTGCG TGGGTATGTC ACACTGCTCT CAGTCCAACA GACGACCCAA 
GTTCAGCGCC TTCAGGGACG TCCATTGAAT ATCGCTTTAA TAAACGGCGA TTTATCTGAG 
AAATATCGCC ATGTGGGCTT TAATAGGCCT GGAAACATCA AGCACATCAC CGATTTGTTC 
ATCACTGAGG AATCCTGGAT TGAAAATGCA TACAAACTCC TGCATGATTC CAGTATTGAT 
GTTGTGTTTG TGAGCGGTGT GGTTGATGTG GATCTGAAAC AGAGATGCAA AGACGTTCTG 
ATTCTTGAAG GTGTTAAAAG CAGCGTTTTG AAGCATTTCA GCACATGCAC CGGAGCCGTC 
CCGGTGTCCT ACATCTGTCA GCTGGATGAG CGCCGCGTGG GCCGAGGGCT GAGGCTCAGG 
AAAGAGTCGA TCAGTGGGAG ATCTGATATT GTGAGCATCA CCACTGACTG CTCGGTTCTG 
GTCACTGCGG TTATATGCAG CTCTGTGTCT GCTAAACTGC AGATGCTGGA GGATGAGTTC 
TGGAGCTGCG CGCACCGGCT GCAGCATGCG CTCACAGATG GGAAACTTCT GCATGGCGCT 
GGAGTCACTG AACTCATCTG CATACGCCGG CTTCGACAGT ACACACAGCT GCACCCTGAC 
AGCCCTCATG ACAGTGCGGT CCTGGAGCTG ATGTCTGAAG CCTGGATGGA CTTCATCTCT 
ACTGTAATGC TGAACAGCGG CTCGGTGGCG AATAGAACAG AGGCCTGGAC GTCTATCACA 
CATCAGATGA GACGACACAC AGATATAGGA GTCACAGATG GACTCGGTGT GTATGATAAC 
GTGACGGTGA AGTGTGAAGC CTGGAGAAGA GCTCTGGATC TGGTGTTTCT GGTGCTTCAG 
TCGGACACTG AGATCATTAC TGGGGTCAGT CAGGGAGAAA ACACGTATAA TCAGCTGATG 
TTTCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336756.1
CDS	52..2340
Translation	MDPSCKQCSFAMKRRHIGLQQLQAITSTAHAFLGPNKRLKFIQDEDAGDAVLIGSCPRLLEHLELDGSVGQLLHETVLAQKKLFHSGTNTLMFLAGAWSRVALECLNRGISVSDIKSAMRGGLQECLDACTQLAVSVEEFKKSDVRSNDQCIDMMVKHSRYINARTKNTQSDDNVQDFRKLSIKSDDQCMDMTHKHSRYANTQTEHAQSDDNVQDFKGSGIKSNDQCMDMTLKHSRYINTKTEHAQSNVNMQDFRKLDDKSDDQCKHMTLKHSRHFNARTKNTQSDVNVQDLKGSDIKSIDQCMDMTLKHSTHFNAQTEHNMQDFRKLDDKSDDQCMYMTLKHSRHFNAQTEHTQSEVTMQDFTKSDIKLDNQSMHMTLKHSRHFNAQTEHTHHDVTVIAQAVSHGCSSMHLLLKAYELQNRAHLDIHNLATCCIPGVSEDHACVLRGYVTLLSVQQTTQVQRLQGRPLNIALINGDLSEKYRHVGFNRPGNIKHITDLFITEESWIENAYKLLHDSSIDVVFVSGVVDVDLKQRCKDVLILEGVKSSVLKHFSTCTGAVPVSYICQLDERRVGRGLRLRKESISGRSDIVSITTDCSVLVTAVICSSVSAKLQMLEDEFWSCAHRLQHALTDGKLLHGAGVTELICIRRLRQYTQLHPDSPHDSAVLELMSEAWMDFISTVMLNSGSVANRTEAWTSITHQMRRHTDIGVTDGLGVYDNVTVKCEAWRRALDLVFLVLQSDTEIITGVSQGENTYNQLMFL

Target ORF information:

RefSeq Version	XM_021481081.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Bardet-Biedl syndrome 12 (bbs12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481081.1

ORF Insert Sequence:

ATGGACCCGA GTTGTAAACA GTGCAGCTTT GCGATGAAGC GGCGTCACAT CGGCCTCCAG 
CAGCTGCAGG CCATCACATC CACCGCTCAT GCATTTCTAG GTCCAAACAA ACGGCTGAAG 
TTCATTCAAG ATGAAGACGC CGGGGATGCA GTTTTAATCG GATCCTGTCC ACGTCTGCTG 
GAACACCTGG AGCTGGACGG ATCTGTCGGA CAGCTGCTGC ACGAGACAGT TCTGGCCCAG 
AAGAAGCTCT TCCACTCGGG CACGAACACG CTGATGTTTC TGGCCGGAGC CTGGAGCCGA 
GTCGCTCTGG AGTGTCTGAA CAGAGGAATC AGTGTGTCGG ACATTAAATC AGCCATGAGG 
GGAGGACTGC AGGAGTGTTT GGATGCATGC ACACAGTTAG CGGTCAGTGT GGAGGAGTTT 
AAGAAATCTG ATGTTAGATC TAATGATCAG TGCATTGATA TGATGGTCAA ACACAGCAGA 
TACATTAATG CACGAACTAA AAACACACAA TCTGATGACA ATGTGCAAGA TTTTAGAAAG 
TTAAGTATTA AATCAGATGA TCAGTGCATG GATATGACAC ATAAACATAG CAGATATGCT 
AATACACAAA CTGAACACGC ACAATCAGAT GACAATGTGC AAGATTTTAA GGGATCAGGT 
ATTAAATCTA ATGATCAATG CATGGATATG ACACTCAAAC ACAGCAGATA CATTAATACA 
AAAACGGAAC ACGCACAATC AAATGTCAAT ATGCAAGATT TTAGGAAGTT AGATGATAAA 
TCTGATGATC AATGCAAGCA TATGACACTC AAACACAGCA GACACTTTAA TGCACGAACT 
AAAAACACAC AATCAGATGT CAATGTGCAA GATTTAAAGG GATCAGATAT TAAATCTATT 
GATCAATGCA TGGATATGAC ACTGAAACAC AGCACACACT TTAATGCACA AACTGAACAC 
AATATGCAAG ATTTTAGGAA GTTAGATGAT AAATCTGATG ATCAATGCAT GTATATGACA 
CTCAAACACA GCAGACACTT TAATGCACAA ACTGAACACA CACAATCAGA AGTTACTATG 
CAAGATTTTA CGAAATCAGA TATTAAATTG GACAATCAAA GCATGCATAT GACCCTCAAA 
CACAGCAGAC ACTTTAATGC ACAAACCGAA CACACACATC ATGATGTCAC TGTCATCGCA 
CAAGCCGTCA GCCACGGATG CTCCTCCATG CATTTACTTT TAAAAGCGTA TGAGTTGCAA 
AACCGCGCAC ATCTGGATAT ACACAATCTG GCAACGTGCT GCATCCCAGG CGTATCTGAA 
GATCATGCGT GTGTGTTGCG TGGGTATGTC ACACTGCTCT CAGTCCAACA GACGACCCAA 
GTTCAGCGCC TTCAGGGACG TCCATTGAAT ATCGCTTTAA TAAACGGCGA TTTATCTGAG 
AAATATCGCC ATGTGGGCTT TAATAGGCCT GGAAACATCA AGCACATCAC CGATTTGTTC 
ATCACTGAGG AATCCTGGAT TGAAAATGCA TACAAACTCC TGCATGATTC CAGTATTGAT 
GTTGTGTTTG TGAGCGGTGT GGTTGATGTG GATCTGAAAC AGAGATGCAA AGACGTTCTG 
ATTCTTGAAG GTGTTAAAAG CAGCGTTTTG AAGCATTTCA GCACATGCAC CGGAGCCGTC 
CCGGTGTCCT ACATCTGTCA GCTGGATGAG CGCCGCGTGG GCCGAGGGCT GAGGCTCAGG 
AAAGAGTCGA TCAGTGGGAG ATCTGATATT GTGAGCATCA CCACTGACTG CTCGGTTCTG 
GTCACTGCGG TTATATGCAG CTCTGTGTCT GCTAAACTGC AGATGCTGGA GGATGAGTTC 
TGGAGCTGCG CGCACCGGCT GCAGCATGCG CTCACAGATG GGAAACTTCT GCATGGCGCT 
GGAGTCACTG AACTCATCTG CATACGCCGG CTTCGACAGT ACACACAGCT GCACCCTGAC 
AGCCCTCATG ACAGTGCGGT CCTGGAGCTG ATGTCTGAAG CCTGGATGGA CTTCATCTCT 
ACTGTAATGC TGAACAGCGG CTCGGTGGCG AATAGAACAG AGGCCTGGAC GTCTATCACA 
CATCAGATGA GACGACACAC AGATATAGGA GTCACAGATG GACTCGGTGT GTATGATAAC 
GTGACGGTGA AGTGTGAAGC CTGGAGAAGA GCTCTGGATC TGGTGTTTCT GGTGCTTCAG 
TCGGACACTG AGATCATTAC TGGGGTCAGT CAGGGAGAAA ACACGTATAA TCAGCTGATG 
TTTCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BBS12 ( XM_002667206.5 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_002667252.1 Danio rerio Bardet-Biedl syndrome 12 (bbs12), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa19231
Clone ID Related Accession (Same CDS sequence)	XM_002667206.5 , XM_002667206.4
Accession Version	XM_002667206.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	Bardet-Biedl syndrome 12 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002667206.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCGA GTTGTAAACA GTGCAGCTTT GCGATGAAGC GGCGTCACAT CGGCCTCCAG 
CAGCTGCAGG CCATCACATC CACCGCTCAT GCATTTCTAG GTCCAAACAA ACGGCTGAAG 
TTCATTCAAG ATGAAGACGC CGGGGATGCA GTTTTAATCG GATCCTGTCC ACGTCTGCTG 
GAACACCTGG AGCTGGACGG ATCTGTCGGA CAGCTGCTGC ACGAGACAGT TCTGGCCCAG 
AAGAAGCTCT TCCACTCGGG CACGAACACG CTGATGTTTC TGGCCGGAGC CTGGAGCCGA 
GTCGCTCTGG AGTGTCTGAA CAGAGGAATC AGTGTGTCGG ACATTAAATC AGCCATGAGG 
GGAGGACTGC AGGAGTGTTT GGATGCATGC ACACAGTTAG CGGTCAGTGT GGAGGAGTTT 
AAGAAATCTG ATGTTAGATC TAATGATCAG TGCATTGATA TGATGGTCAA ACACAGCAGA 
TACATTAATG CACGAACTAA AAACACACAA TCTGATGACA ATGTGCAAGA TTTTAGAAAG 
TTAAGTATTA AATCAGATGA TCAGTGCATG GATATGACAC ATAAACATAG CAGATATGCT 
AATACACAAA CTGAACACGC ACAATCAGAT GACAATGTGC AAGATTTTAA GGGATCAGGT 
ATTAAATCTA ATGATCAATG CATGGATATG ACACTCAAAC ACAGCAGATA CATTAATACA 
AAAACGGAAC ACGCACAATC AAATGTCAAT ATGCAAGATT TTAGGAAGTT AGATGATAAA 
TCTGATGATC AATGCAAGCA TATGACACTC AAACACAGCA GACACTTTAA TGCACGAACT 
AAAAACACAC AATCAGATGT CAATGTGCAA GATTTAAAGG GATCAGATAT TAAATCTATT 
GATCAATGCA TGGATATGAC ACTGAAACAC AGCACACACT TTAATGCACA AACTGAACAC 
AATATGCAAG ATTTTAGGAA GTTAGATGAT AAATCTGATG ATCAATGCAT GTATATGACA 
CTCAAACACA GCAGACACTT TAATGCACAA ACTGAACACA CACAATCAGA AGTTACTATG 
CAAGATTTTA CGAAATCAGA TATTAAATTG GACAATCAAA GCATGCATAT GACCCTCAAA 
CACAGCAGAC ACTTTAATGC ACAAACCGAA CACACACATC ATGATGTCAC TGTCATCGCA 
CAAGCCGTCA GCCACGGATG CTCCTCCATG CATTTACTTT TAAAAGCGTA TGAGTTGCAA 
AACCGCGCAC ATCTGGATAT ACACAATCTG GCAACGTGCT GCATCCCAGG CGTATCTGAA 
GATCATGCGT GTGTGTTGCG TGGGTATGTC ACACTGCTCT CAGTCCAACA GACGACCCAA 
GTTCAGCGCC TTCAGGGACG TCCATTGAAT ATCGCTTTAA TAAACGGCGA TTTATCTGAG 
AAATATCGCC ATGTGGGCTT TAATAGGCCT GGAAACATCA AGCACATCAC CGATTTGTTC 
ATCACTGAGG AATCCTGGAT TGAAAATGCA TACAAACTCC TGCATGATTC CAGTATTGAT 
GTTGTGTTTG TGAGCGGTGT GGTTGATGTG GATCTGAAAC AGAGATGCAA AGACGTTCTG 
ATTCTTGAAG GTGTTAAAAG CAGCGTTTTG AAGCATTTCA GCACATGCAC CGGAGCCGTC 
CCGGTGTCCT ACATCTGTCA GCTGGATGAG CGCCGCGTGG GCCGAGGGCT GAGGCTCAGG 
AAAGAGTCGA TCAGTGGGAG ATCTGATATT GTGAGCATCA CCACTGACTG CTCGGTTCTG 
GTCACTGCGG TTATATGCAG CTCTGTGTCT GCTAAACTGC AGATGCTGGA GGATGAGTTC 
TGGAGCTGCG CGCACCGGCT GCAGCATGCG CTCACAGATG GGAAACTTCT GCATGGCGCT 
GGAGTCACTG AACTCATCTG CATACGCCGG CTTCGACAGT ACACACAGCT GCACCCTGAC 
AGAAGCCCTC ATGACAGTGC GGTCCTGGAG CTGATGTCTG AAGCCTGGAT GGACTTCATC 
TCTACTGTAA TGCTGAACAG CGGCTCGGTG GCGAATAGAA CAGAGGCCTG GACGTCTATC 
ACACATCAGA TGAGACGACA CACAGATATA GGAGTCACAG ATGGACTCGG TGTGTATGAT 
AACGTGACGG TGAAGTGTGA AGCCTGGAGA AGAGCTCTGG ATCTGGTGTT TCTGGTGCTT 
CAGTCGGACA CTGAGATCAT TACTGGGGTC AGTCAGGGAG AAAACACGTA TAATCAGCTG 
ATGTTTCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002667252.1
CDS	52..2343
Translation	MDPSCKQCSFAMKRRHIGLQQLQAITSTAHAFLGPNKRLKFIQDEDAGDAVLIGSCPRLLEHLELDGSVGQLLHETVLAQKKLFHSGTNTLMFLAGAWSRVALECLNRGISVSDIKSAMRGGLQECLDACTQLAVSVEEFKKSDVRSNDQCIDMMVKHSRYINARTKNTQSDDNVQDFRKLSIKSDDQCMDMTHKHSRYANTQTEHAQSDDNVQDFKGSGIKSNDQCMDMTLKHSRYINTKTEHAQSNVNMQDFRKLDDKSDDQCKHMTLKHSRHFNARTKNTQSDVNVQDLKGSDIKSIDQCMDMTLKHSTHFNAQTEHNMQDFRKLDDKSDDQCMYMTLKHSRHFNAQTEHTQSEVTMQDFTKSDIKLDNQSMHMTLKHSRHFNAQTEHTHHDVTVIAQAVSHGCSSMHLLLKAYELQNRAHLDIHNLATCCIPGVSEDHACVLRGYVTLLSVQQTTQVQRLQGRPLNIALINGDLSEKYRHVGFNRPGNIKHITDLFITEESWIENAYKLLHDSSIDVVFVSGVVDVDLKQRCKDVLILEGVKSSVLKHFSTCTGAVPVSYICQLDERRVGRGLRLRKESISGRSDIVSITTDCSVLVTAVICSSVSAKLQMLEDEFWSCAHRLQHALTDGKLLHGAGVTELICIRRLRQYTQLHPDRSPHDSAVLELMSEAWMDFISTVMLNSGSVANRTEAWTSITHQMRRHTDIGVTDGLGVYDNVTVKCEAWRRALDLVFLVLQSDTEIITGVSQGENTYNQLMFL

Target ORF information:

RefSeq Version	XM_002667206.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Bardet-Biedl syndrome 12 (bbs12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002667206.5

ORF Insert Sequence:

ATGGACCCGA GTTGTAAACA GTGCAGCTTT GCGATGAAGC GGCGTCACAT CGGCCTCCAG 
CAGCTGCAGG CCATCACATC CACCGCTCAT GCATTTCTAG GTCCAAACAA ACGGCTGAAG 
TTCATTCAAG ATGAAGACGC CGGGGATGCA GTTTTAATCG GATCCTGTCC ACGTCTGCTG 
GAACACCTGG AGCTGGACGG ATCTGTCGGA CAGCTGCTGC ACGAGACAGT TCTGGCCCAG 
AAGAAGCTCT TCCACTCGGG CACGAACACG CTGATGTTTC TGGCCGGAGC CTGGAGCCGA 
GTCGCTCTGG AGTGTCTGAA CAGAGGAATC AGTGTGTCGG ACATTAAATC AGCCATGAGG 
GGAGGACTGC AGGAGTGTTT GGATGCATGC ACACAGTTAG CGGTCAGTGT GGAGGAGTTT 
AAGAAATCTG ATGTTAGATC TAATGATCAG TGCATTGATA TGATGGTCAA ACACAGCAGA 
TACATTAATG CACGAACTAA AAACACACAA TCTGATGACA ATGTGCAAGA TTTTAGAAAG 
TTAAGTATTA AATCAGATGA TCAGTGCATG GATATGACAC ATAAACATAG CAGATATGCT 
AATACACAAA CTGAACACGC ACAATCAGAT GACAATGTGC AAGATTTTAA GGGATCAGGT 
ATTAAATCTA ATGATCAATG CATGGATATG ACACTCAAAC ACAGCAGATA CATTAATACA 
AAAACGGAAC ACGCACAATC AAATGTCAAT ATGCAAGATT TTAGGAAGTT AGATGATAAA 
TCTGATGATC AATGCAAGCA TATGACACTC AAACACAGCA GACACTTTAA TGCACGAACT 
AAAAACACAC AATCAGATGT CAATGTGCAA GATTTAAAGG GATCAGATAT TAAATCTATT 
GATCAATGCA TGGATATGAC ACTGAAACAC AGCACACACT TTAATGCACA AACTGAACAC 
AATATGCAAG ATTTTAGGAA GTTAGATGAT AAATCTGATG ATCAATGCAT GTATATGACA 
CTCAAACACA GCAGACACTT TAATGCACAA ACTGAACACA CACAATCAGA AGTTACTATG 
CAAGATTTTA CGAAATCAGA TATTAAATTG GACAATCAAA GCATGCATAT GACCCTCAAA 
CACAGCAGAC ACTTTAATGC ACAAACCGAA CACACACATC ATGATGTCAC TGTCATCGCA 
CAAGCCGTCA GCCACGGATG CTCCTCCATG CATTTACTTT TAAAAGCGTA TGAGTTGCAA 
AACCGCGCAC ATCTGGATAT ACACAATCTG GCAACGTGCT GCATCCCAGG CGTATCTGAA 
GATCATGCGT GTGTGTTGCG TGGGTATGTC ACACTGCTCT CAGTCCAACA GACGACCCAA 
GTTCAGCGCC TTCAGGGACG TCCATTGAAT ATCGCTTTAA TAAACGGCGA TTTATCTGAG 
AAATATCGCC ATGTGGGCTT TAATAGGCCT GGAAACATCA AGCACATCAC CGATTTGTTC 
ATCACTGAGG AATCCTGGAT TGAAAATGCA TACAAACTCC TGCATGATTC CAGTATTGAT 
GTTGTGTTTG TGAGCGGTGT GGTTGATGTG GATCTGAAAC AGAGATGCAA AGACGTTCTG 
ATTCTTGAAG GTGTTAAAAG CAGCGTTTTG AAGCATTTCA GCACATGCAC CGGAGCCGTC 
CCGGTGTCCT ACATCTGTCA GCTGGATGAG CGCCGCGTGG GCCGAGGGCT GAGGCTCAGG 
AAAGAGTCGA TCAGTGGGAG ATCTGATATT GTGAGCATCA CCACTGACTG CTCGGTTCTG 
GTCACTGCGG TTATATGCAG CTCTGTGTCT GCTAAACTGC AGATGCTGGA GGATGAGTTC 
TGGAGCTGCG CGCACCGGCT GCAGCATGCG CTCACAGATG GGAAACTTCT GCATGGCGCT 
GGAGTCACTG AACTCATCTG CATACGCCGG CTTCGACAGT ACACACAGCT GCACCCTGAC 
AGAAGCCCTC ATGACAGTGC GGTCCTGGAG CTGATGTCTG AAGCCTGGAT GGACTTCATC 
TCTACTGTAA TGCTGAACAG CGGCTCGGTG GCGAATAGAA CAGAGGCCTG GACGTCTATC 
ACACATCAGA TGAGACGACA CACAGATATA GGAGTCACAG ATGGACTCGG TGTGTATGAT 
AACGTGACGG TGAAGTGTGA AGCCTGGAGA AGAGCTCTGG ATCTGGTGTT TCTGGTGCTT 
CAGTCGGACA CTGAGATCAT TACTGGGGTC AGTCAGGGAG AAAACACGTA TAATCAGCTG 
ATGTTTCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BBS12 ( XM_002667206.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_002667252.1 Danio rerio Bardet-Biedl syndrome 12 (bbs12), transcript variant X1, mRNA.

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CloneID	ODa19231
Clone ID Related Accession (Same CDS sequence)	XM_002667206.5 , XM_002667206.4
Accession Version	XM_002667206.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2292bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	Bardet-Biedl syndrome 12 protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002667206.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCGA GTTGTAAACA GTGCAGCTTT GCGATGAAGC GGCGTCACAT CGGCCTCCAG 
CAGCTGCAGG CCATCACATC CACCGCTCAT GCATTTCTAG GTCCAAACAA ACGGCTGAAG 
TTCATTCAAG ATGAAGACGC CGGGGATGCA GTTTTAATCG GATCCTGTCC ACGTCTGCTG 
GAACACCTGG AGCTGGACGG ATCTGTCGGA CAGCTGCTGC ACGAGACAGT TCTGGCCCAG 
AAGAAGCTCT TCCACTCGGG CACGAACACG CTGATGTTTC TGGCCGGAGC CTGGAGCCGA 
GTCGCTCTGG AGTGTCTGAA CAGAGGAATC AGTGTGTCGG ACATTAAATC AGCCATGAGG 
GGAGGACTGC AGGAGTGTTT GGATGCATGC ACACAGTTAG CGGTCAGTGT GGAGGAGTTT 
AAGAAATCTG ATGTTAGATC TAATGATCAG TGCATTGATA TGATGGTCAA ACACAGCAGA 
TACATTAATG CACGAACTAA AAACACACAA TCTGATGACA ATGTGCAAGA TTTTAGAAAG 
TTAAGTATTA AATCAGATGA TCAGTGCATG GATATGACAC ATAAACATAG CAGATATGCT 
AATACACAAA CTGAACACGC ACAATCAGAT GACAATGTGC AAGATTTTAA GGGATCAGGT 
ATTAAATCTA ATGATCAATG CATGGATATG ACACTCAAAC ACAGCAGATA CATTAATACA 
AAAACGGAAC ACGCACAATC AAATGTCAAT ATGCAAGATT TTAGGAAGTT AGATGATAAA 
TCTGATGATC AATGCAAGCA TATGACACTC AAACACAGCA GACACTTTAA TGCACGAACT 
AAAAACACAC AATCAGATGT CAATGTGCAA GATTTAAAGG GATCAGATAT TAAATCTATT 
GATCAATGCA TGGATATGAC ACTGAAACAC AGCACACACT TTAATGCACA AACTGAACAC 
AATATGCAAG ATTTTAGGAA GTTAGATGAT AAATCTGATG ATCAATGCAT GTATATGACA 
CTCAAACACA GCAGACACTT TAATGCACAA ACTGAACACA CACAATCAGA AGTTACTATG 
CAAGATTTTA CGAAATCAGA TATTAAATTG GACAATCAAA GCATGCATAT GACCCTCAAA 
CACAGCAGAC ACTTTAATGC ACAAACCGAA CACACACATC ATGATGTCAC TGTCATCGCA 
CAAGCCGTCA GCCACGGATG CTCCTCCATG CATTTACTTT TAAAAGCGTA TGAGTTGCAA 
AACCGCGCAC ATCTGGATAT ACACAATCTG GCAACGTGCT GCATCCCAGG CGTATCTGAA 
GATCATGCGT GTGTGTTGCG TGGGTATGTC ACACTGCTCT CAGTCCAACA GACGACCCAA 
GTTCAGCGCC TTCAGGGACG TCCATTGAAT ATCGCTTTAA TAAACGGCGA TTTATCTGAG 
AAATATCGCC ATGTGGGCTT TAATAGGCCT GGAAACATCA AGCACATCAC CGATTTGTTC 
ATCACTGAGG AATCCTGGAT TGAAAATGCA TACAAACTCC TGCATGATTC CAGTATTGAT 
GTTGTGTTTG TGAGCGGTGT GGTTGATGTG GATCTGAAAC AGAGATGCAA AGACGTTCTG 
ATTCTTGAAG GTGTTAAAAG CAGCGTTTTG AAGCATTTCA GCACATGCAC CGGAGCCGTC 
CCGGTGTCCT ACATCTGTCA GCTGGATGAG CGCCGCGTGG GCCGAGGGCT GAGGCTCAGG 
AAAGAGTCGA TCAGTGGGAG ATCTGATATT GTGAGCATCA CCACTGACTG CTCGGTTCTG 
GTCACTGCGG TTATATGCAG CTCTGTGTCT GCTAAACTGC AGATGCTGGA GGATGAGTTC 
TGGAGCTGCG CGCACCGGCT GCAGCATGCG CTCACAGATG GGAAACTTCT GCATGGCGCT 
GGAGTCACTG AACTCATCTG CATACGCCGG CTTCGACAGT ACACACAGCT GCACCCTGAC 
AGAAGCCCTC ATGACAGTGC GGTCCTGGAG CTGATGTCTG AAGCCTGGAT GGACTTCATC 
TCTACTGTAA TGCTGAACAG CGGCTCGGTG GCGAATAGAA CAGAGGCCTG GACGTCTATC 
ACACATCAGA TGAGACGACA CACAGATATA GGAGTCACAG ATGGACTCGG TGTGTATGAT 
AACGTGACGG TGAAGTGTGA AGCCTGGAGA AGAGCTCTGG ATCTGGTGTT TCTGGTGCTT 
CAGTCGGACA CTGAGATCAT TACTGGGGTC AGTCAGGGAG AAAACACGTA TAATCAGCTG 
ATGTTTCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002667252.1
CDS	27..2318
Translation	MDPSCKQCSFAMKRRHIGLQQLQAITSTAHAFLGPNKRLKFIQDEDAGDAVLIGSCPRLLEHLELDGSVGQLLHETVLAQKKLFHSGTNTLMFLAGAWSRVALECLNRGISVSDIKSAMRGGLQECLDACTQLAVSVEEFKKSDVRSNDQCIDMMVKHSRYINARTKNTQSDDNVQDFRKLSIKSDDQCMDMTHKHSRYANTQTEHAQSDDNVQDFKGSGIKSNDQCMDMTLKHSRYINTKTEHAQSNVNMQDFRKLDDKSDDQCKHMTLKHSRHFNARTKNTQSDVNVQDLKGSDIKSIDQCMDMTLKHSTHFNAQTEHNMQDFRKLDDKSDDQCMYMTLKHSRHFNAQTEHTQSEVTMQDFTKSDIKLDNQSMHMTLKHSRHFNAQTEHTHHDVTVIAQAVSHGCSSMHLLLKAYELQNRAHLDIHNLATCCIPGVSEDHACVLRGYVTLLSVQQTTQVQRLQGRPLNIALINGDLSEKYRHVGFNRPGNIKHITDLFITEESWIENAYKLLHDSSIDVVFVSGVVDVDLKQRCKDVLILEGVKSSVLKHFSTCTGAVPVSYICQLDERRVGRGLRLRKESISGRSDIVSITTDCSVLVTAVICSSVSAKLQMLEDEFWSCAHRLQHALTDGKLLHGAGVTELICIRRLRQYTQLHPDRSPHDSAVLELMSEAWMDFISTVMLNSGSVANRTEAWTSITHQMRRHTDIGVTDGLGVYDNVTVKCEAWRRALDLVFLVLQSDTEIITGVSQGENTYNQLMFL

Target ORF information:

RefSeq Version	XM_002667206.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Bardet-Biedl syndrome 12 (bbs12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002667206.4

ORF Insert Sequence:

ATGGACCCGA GTTGTAAACA GTGCAGCTTT GCGATGAAGC GGCGTCACAT CGGCCTCCAG 
CAGCTGCAGG CCATCACATC CACCGCTCAT GCATTTCTAG GTCCAAACAA ACGGCTGAAG 
TTCATTCAAG ATGAAGACGC CGGGGATGCA GTTTTAATCG GATCCTGTCC ACGTCTGCTG 
GAACACCTGG AGCTGGACGG ATCTGTCGGA CAGCTGCTGC ACGAGACAGT TCTGGCCCAG 
AAGAAGCTCT TCCACTCGGG CACGAACACG CTGATGTTTC TGGCCGGAGC CTGGAGCCGA 
GTCGCTCTGG AGTGTCTGAA CAGAGGAATC AGTGTGTCGG ACATTAAATC AGCCATGAGG 
GGAGGACTGC AGGAGTGTTT GGATGCATGC ACACAGTTAG CGGTCAGTGT GGAGGAGTTT 
AAGAAATCTG ATGTTAGATC TAATGATCAG TGCATTGATA TGATGGTCAA ACACAGCAGA 
TACATTAATG CACGAACTAA AAACACACAA TCTGATGACA ATGTGCAAGA TTTTAGAAAG 
TTAAGTATTA AATCAGATGA TCAGTGCATG GATATGACAC ATAAACATAG CAGATATGCT 
AATACACAAA CTGAACACGC ACAATCAGAT GACAATGTGC AAGATTTTAA GGGATCAGGT 
ATTAAATCTA ATGATCAATG CATGGATATG ACACTCAAAC ACAGCAGATA CATTAATACA 
AAAACGGAAC ACGCACAATC AAATGTCAAT ATGCAAGATT TTAGGAAGTT AGATGATAAA 
TCTGATGATC AATGCAAGCA TATGACACTC AAACACAGCA GACACTTTAA TGCACGAACT 
AAAAACACAC AATCAGATGT CAATGTGCAA GATTTAAAGG GATCAGATAT TAAATCTATT 
GATCAATGCA TGGATATGAC ACTGAAACAC AGCACACACT TTAATGCACA AACTGAACAC 
AATATGCAAG ATTTTAGGAA GTTAGATGAT AAATCTGATG ATCAATGCAT GTATATGACA 
CTCAAACACA GCAGACACTT TAATGCACAA ACTGAACACA CACAATCAGA AGTTACTATG 
CAAGATTTTA CGAAATCAGA TATTAAATTG GACAATCAAA GCATGCATAT GACCCTCAAA 
CACAGCAGAC ACTTTAATGC ACAAACCGAA CACACACATC ATGATGTCAC TGTCATCGCA 
CAAGCCGTCA GCCACGGATG CTCCTCCATG CATTTACTTT TAAAAGCGTA TGAGTTGCAA 
AACCGCGCAC ATCTGGATAT ACACAATCTG GCAACGTGCT GCATCCCAGG CGTATCTGAA 
GATCATGCGT GTGTGTTGCG TGGGTATGTC ACACTGCTCT CAGTCCAACA GACGACCCAA 
GTTCAGCGCC TTCAGGGACG TCCATTGAAT ATCGCTTTAA TAAACGGCGA TTTATCTGAG 
AAATATCGCC ATGTGGGCTT TAATAGGCCT GGAAACATCA AGCACATCAC CGATTTGTTC 
ATCACTGAGG AATCCTGGAT TGAAAATGCA TACAAACTCC TGCATGATTC CAGTATTGAT 
GTTGTGTTTG TGAGCGGTGT GGTTGATGTG GATCTGAAAC AGAGATGCAA AGACGTTCTG 
ATTCTTGAAG GTGTTAAAAG CAGCGTTTTG AAGCATTTCA GCACATGCAC CGGAGCCGTC 
CCGGTGTCCT ACATCTGTCA GCTGGATGAG CGCCGCGTGG GCCGAGGGCT GAGGCTCAGG 
AAAGAGTCGA TCAGTGGGAG ATCTGATATT GTGAGCATCA CCACTGACTG CTCGGTTCTG 
GTCACTGCGG TTATATGCAG CTCTGTGTCT GCTAAACTGC AGATGCTGGA GGATGAGTTC 
TGGAGCTGCG CGCACCGGCT GCAGCATGCG CTCACAGATG GGAAACTTCT GCATGGCGCT 
GGAGTCACTG AACTCATCTG CATACGCCGG CTTCGACAGT ACACACAGCT GCACCCTGAC 
AGAAGCCCTC ATGACAGTGC GGTCCTGGAG CTGATGTCTG AAGCCTGGAT GGACTTCATC 
TCTACTGTAA TGCTGAACAG CGGCTCGGTG GCGAATAGAA CAGAGGCCTG GACGTCTATC 
ACACATCAGA TGAGACGACA CACAGATATA GGAGTCACAG ATGGACTCGG TGTGTATGAT 
AACGTGACGG TGAAGTGTGA AGCCTGGAGA AGAGCTCTGG ATCTGGTGTT TCTGGTGCTT 
CAGTCGGACA CTGAGATCAT TACTGGGGTC AGTCAGGGAG AAAACACGTA TAATCAGCTG 
ATGTTTCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BBS12 ( XM_009307657.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009305932.1 Danio rerio Bardet-Biedl syndrome 12 (bbs12), transcript variant X2, mRNA.

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CloneID	ODa45989
Clone ID Related Accession (Same CDS sequence)	XM_009307657.2
Accession Version	XM_009307657.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	Bardet-Biedl syndrome 12 protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307657.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAACAG TCAGACACTG GCGGCGTCAC ATCGGCCTCC AGCAGCTGCA GGCCATCACA 
TCCACCGCTC ATGCATTTCT AGGTCCAAAC AAACGGCTGA AGTTCATTCA AGATGAAGAC 
GCCGGGGATG CAGTTTTAAT CGGATCCTGT CCACGTCTGC TGGAACACCT GGAGCTGGAC 
GGATCTGTCG GACAGCTGCT GCACGAGACA GTTCTGGCCC AGAAGAAGCT CTTCCACTCG 
GGCACGAACA CGCTGATGTT TCTGGCCGGA GCCTGGAGCC GAGTCGCTCT GGAGTGTCTG 
AACAGAGGAA TCAGTGTGTC GGACATTAAA TCAGCCATGA GGGGAGGACT GCAGGAGTGT 
TTGGATGCAT GCACACAGTT AGCGGTCAGT GTGGAGGAGT TTAAGAAATC TGATGTTAGA 
TCTAATGATC AGTGCATTGA TATGATGGTC AAACACAGCA GATACATTAA TGCACGAACT 
AAAAACACAC AATCTGATGA CAATGTGCAA GATTTTAGAA AGTTAAGTAT TAAATCAGAT 
GATCAGTGCA TGGATATGAC ACATAAACAT AGCAGATATG CTAATACACA AACTGAACAC 
GCACAATCAG ATGACAATGT GCAAGATTTT AAGGGATCAG GTATTAAATC TAATGATCAA 
TGCATGGATA TGACACTCAA ACACAGCAGA TACATTAATA CAAAAACGGA ACACGCACAA 
TCAAATGTCA ATATGCAAGA TTTTAGGAAG TTAGATGATA AATCTGATGA TCAATGCAAG 
CATATGACAC TCAAACACAG CAGACACTTT AATGCACGAA CTAAAAACAC ACAATCAGAT 
GTCAATGTGC AAGATTTAAA GGGATCAGAT ATTAAATCTA TTGATCAATG CATGGATATG 
ACACTGAAAC ACAGCACACA CTTTAATGCA CAAACTGAAC ACAATATGCA AGATTTTAGG 
AAGTTAGATG ATAAATCTGA TGATCAATGC ATGTATATGA CACTCAAACA CAGCAGACAC 
TTTAATGCAC AAACTGAACA CACACAATCA GAAGTTACTA TGCAAGATTT TACGAAATCA 
GATATTAAAT TGGACAATCA AAGCATGCAT ATGACCCTCA AACACAGCAG ACACTTTAAT 
GCACAAACCG AACACACACA TCATGATGTC ACTGTCATCG CACAAGCCGT CAGCCACGGA 
TGCTCCTCCA TGCATTTACT TTTAAAAGCG TATGAGTTGC AAAACCGCGC ACATCTGGAT 
ATACACAATC TGGCAACGTG CTGCATCCCA GGCGTATCTG AAGATCATGC GTGTGTGTTG 
CGTGGGTATG TCACACTGCT CTCAGTCCAA CAGACGACCC AAGTTCAGCG CCTTCAGGGA 
CGTCCATTGA ATATCGCTTT AATAAACGGC GATTTATCTG AGAAATATCG CCATGTGGGC 
TTTAATAGGC CTGGAAACAT CAAGCACATC ACCGATTTGT TCATCACTGA GGAATCCTGG 
ATTGAAAATG CATACAAACT CCTGCATGAT TCCAGTATTG ATGTTGTGTT TGTGAGCGGT 
GTGGTTGATG TGGATCTGAA ACAGAGATGC AAAGACGTTC TGATTCTTGA AGGTGTTAAA 
AGCAGCGTTT TGAAGCATTT CAGCACATGC ACCGGAGCCG TCCCGGTGTC CTACATCTGT 
CAGCTGGATG AGCGCCGCGT GGGCCGAGGG CTGAGGCTCA GGAAAGAGTC GATCAGTGGG 
AGATCTGATA TTGTGAGCAT CACCACTGAC TGCTCGGTTC TGGTCACTGC GGTTATATGC 
AGCTCTGTGT CTGCTAAACT GCAGATGCTG GAGGATGAGT TCTGGAGCTG CGCGCACCGG 
CTGCAGCATG CGCTCACAGA TGGGAAACTT CTGCATGGCG CTGGAGTCAC TGAACTCATC 
TGCATACGCC GGCTTCGACA GTACACACAG CTGCACCCTG ACAGAAGCCC TCATGACAGT 
GCGGTCCTGG AGCTGATGTC TGAAGCCTGG ATGGACTTCA TCTCTACTGT AATGCTGAAC 
AGCGGCTCGG TGGCGAATAG AACAGAGGCC TGGACGTCTA TCACACATCA GATGAGACGA 
CACACAGATA TAGGAGTCAC AGATGGACTC GGTGTGTATG ATAACGTGAC GGTGAAGTGT 
GAAGCCTGGA GAAGAGCTCT GGATCTGGTG TTTCTGGTGC TTCAGTCGGA CACTGAGATC 
ATTACTGGGG TCAGTCAGGG AGAAAACACG TATAATCAGC TGATGTTTCT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305932.1
CDS	30..2303
Translation	MTTVRHWRRHIGLQQLQAITSTAHAFLGPNKRLKFIQDEDAGDAVLIGSCPRLLEHLELDGSVGQLLHETVLAQKKLFHSGTNTLMFLAGAWSRVALECLNRGISVSDIKSAMRGGLQECLDACTQLAVSVEEFKKSDVRSNDQCIDMMVKHSRYINARTKNTQSDDNVQDFRKLSIKSDDQCMDMTHKHSRYANTQTEHAQSDDNVQDFKGSGIKSNDQCMDMTLKHSRYINTKTEHAQSNVNMQDFRKLDDKSDDQCKHMTLKHSRHFNARTKNTQSDVNVQDLKGSDIKSIDQCMDMTLKHSTHFNAQTEHNMQDFRKLDDKSDDQCMYMTLKHSRHFNAQTEHTQSEVTMQDFTKSDIKLDNQSMHMTLKHSRHFNAQTEHTHHDVTVIAQAVSHGCSSMHLLLKAYELQNRAHLDIHNLATCCIPGVSEDHACVLRGYVTLLSVQQTTQVQRLQGRPLNIALINGDLSEKYRHVGFNRPGNIKHITDLFITEESWIENAYKLLHDSSIDVVFVSGVVDVDLKQRCKDVLILEGVKSSVLKHFSTCTGAVPVSYICQLDERRVGRGLRLRKESISGRSDIVSITTDCSVLVTAVICSSVSAKLQMLEDEFWSCAHRLQHALTDGKLLHGAGVTELICIRRLRQYTQLHPDRSPHDSAVLELMSEAWMDFISTVMLNSGSVANRTEAWTSITHQMRRHTDIGVTDGLGVYDNVTVKCEAWRRALDLVFLVLQSDTEIITGVSQGENTYNQLMFL

Target ORF information:

RefSeq Version	XM_009307657.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Bardet-Biedl syndrome 12 (bbs12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307657.2

ORF Insert Sequence:

ATGACAACAG TCAGACACTG GCGGCGTCAC ATCGGCCTCC AGCAGCTGCA GGCCATCACA 
TCCACCGCTC ATGCATTTCT AGGTCCAAAC AAACGGCTGA AGTTCATTCA AGATGAAGAC 
GCCGGGGATG CAGTTTTAAT CGGATCCTGT CCACGTCTGC TGGAACACCT GGAGCTGGAC 
GGATCTGTCG GACAGCTGCT GCACGAGACA GTTCTGGCCC AGAAGAAGCT CTTCCACTCG 
GGCACGAACA CGCTGATGTT TCTGGCCGGA GCCTGGAGCC GAGTCGCTCT GGAGTGTCTG 
AACAGAGGAA TCAGTGTGTC GGACATTAAA TCAGCCATGA GGGGAGGACT GCAGGAGTGT 
TTGGATGCAT GCACACAGTT AGCGGTCAGT GTGGAGGAGT TTAAGAAATC TGATGTTAGA 
TCTAATGATC AGTGCATTGA TATGATGGTC AAACACAGCA GATACATTAA TGCACGAACT 
AAAAACACAC AATCTGATGA CAATGTGCAA GATTTTAGAA AGTTAAGTAT TAAATCAGAT 
GATCAGTGCA TGGATATGAC ACATAAACAT AGCAGATATG CTAATACACA AACTGAACAC 
GCACAATCAG ATGACAATGT GCAAGATTTT AAGGGATCAG GTATTAAATC TAATGATCAA 
TGCATGGATA TGACACTCAA ACACAGCAGA TACATTAATA CAAAAACGGA ACACGCACAA 
TCAAATGTCA ATATGCAAGA TTTTAGGAAG TTAGATGATA AATCTGATGA TCAATGCAAG 
CATATGACAC TCAAACACAG CAGACACTTT AATGCACGAA CTAAAAACAC ACAATCAGAT 
GTCAATGTGC AAGATTTAAA GGGATCAGAT ATTAAATCTA TTGATCAATG CATGGATATG 
ACACTGAAAC ACAGCACACA CTTTAATGCA CAAACTGAAC ACAATATGCA AGATTTTAGG 
AAGTTAGATG ATAAATCTGA TGATCAATGC ATGTATATGA CACTCAAACA CAGCAGACAC 
TTTAATGCAC AAACTGAACA CACACAATCA GAAGTTACTA TGCAAGATTT TACGAAATCA 
GATATTAAAT TGGACAATCA AAGCATGCAT ATGACCCTCA AACACAGCAG ACACTTTAAT 
GCACAAACCG AACACACACA TCATGATGTC ACTGTCATCG CACAAGCCGT CAGCCACGGA 
TGCTCCTCCA TGCATTTACT TTTAAAAGCG TATGAGTTGC AAAACCGCGC ACATCTGGAT 
ATACACAATC TGGCAACGTG CTGCATCCCA GGCGTATCTG AAGATCATGC GTGTGTGTTG 
CGTGGGTATG TCACACTGCT CTCAGTCCAA CAGACGACCC AAGTTCAGCG CCTTCAGGGA 
CGTCCATTGA ATATCGCTTT AATAAACGGC GATTTATCTG AGAAATATCG CCATGTGGGC 
TTTAATAGGC CTGGAAACAT CAAGCACATC ACCGATTTGT TCATCACTGA GGAATCCTGG 
ATTGAAAATG CATACAAACT CCTGCATGAT TCCAGTATTG ATGTTGTGTT TGTGAGCGGT 
GTGGTTGATG TGGATCTGAA ACAGAGATGC AAAGACGTTC TGATTCTTGA AGGTGTTAAA 
AGCAGCGTTT TGAAGCATTT CAGCACATGC ACCGGAGCCG TCCCGGTGTC CTACATCTGT 
CAGCTGGATG AGCGCCGCGT GGGCCGAGGG CTGAGGCTCA GGAAAGAGTC GATCAGTGGG 
AGATCTGATA TTGTGAGCAT CACCACTGAC TGCTCGGTTC TGGTCACTGC GGTTATATGC 
AGCTCTGTGT CTGCTAAACT GCAGATGCTG GAGGATGAGT TCTGGAGCTG CGCGCACCGG 
CTGCAGCATG CGCTCACAGA TGGGAAACTT CTGCATGGCG CTGGAGTCAC TGAACTCATC 
TGCATACGCC GGCTTCGACA GTACACACAG CTGCACCCTG ACAGAAGCCC TCATGACAGT 
GCGGTCCTGG AGCTGATGTC TGAAGCCTGG ATGGACTTCA TCTCTACTGT AATGCTGAAC 
AGCGGCTCGG TGGCGAATAG AACAGAGGCC TGGACGTCTA TCACACATCA GATGAGACGA 
CACACAGATA TAGGAGTCAC AGATGGACTC GGTGTGTATG ATAACGTGAC GGTGAAGTGT 
GAAGCCTGGA GAAGAGCTCT GGATCTGGTG TTTCTGGTGC TTCAGTCGGA CACTGAGATC 
ATTACTGGGG TCAGTCAGGG AGAAAACACG TATAATCAGC TGATGTTTCT TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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BBS12 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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