ap4e1 cDNA ORF clone, Danio rerio(zebrafish)

The following ap4e1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ap4e1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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ODa29084 XM_693950.7
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Danio rerio adaptor-related protein complex 4, epsilon 1 subunit (ap4e1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
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ODa29084 XM_693950.6 Danio rerio adaptor-related protein complex 4, epsilon 1 subunit (ap4e1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa29084
    Clone ID Related Accession (Same CDS sequence) XM_693950.6 , XM_693950.7
    Accession Version XM_693950.6 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3366bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product AP-4 complex subunit epsilon-1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_693950.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGTGATG TTGTGGAGAA AACTCTCACT GCTCTGCCGA GTTTGTTTGA TTCTCAAACC 
    GGAGCCGCAA AAATCAGCAA CAGACTCAAA CAGCTGATCA AGAGCATCAC AGAGCTGACC
    TCCAAACATG ACGAGGAGAG TCTCATCAAA CAGGAGCTCT CGGCCATGAA GGAGCAGGTT
    TCGGCTCCCA GCACCAGCAT GAAGCAGATG AGAGAGATCA TGGTGAGGTC CATGTACTGT
    GAGATGCTGG GCTACGACGC CTCCTTCAGC TACATCCATG CCATCAAACT GGCCCAGCAG
    GGCGGCGTCA TGGAGAAGAG AGTCGGGTAT CTGGCAGTGT CTCTTTTCCT CAGTGAAGGC
    CATGAGCTGC TGCTGCTGCT AGTCAACACA GTATTAAAGG ATCTTCAGAG CACCAACCTG
    ATTGAAGCCT GCATGGCCTT GACTGTTGTA GCTCAGGTGT TTCCCAAAGA CATGATTCCT
    GCTGTCCTTC CTCTGGTGGA GGACAAGCTT TCACACCCCA AGGAGATCAT TAGGAGGAAA
    GCTGTCCTGG CCTTATATAA GTTTTATCTC ATCGCTCCTA ATCAAGTGCA GCACATTCAT
    GCCAAGTTCC GGAAGGCTCT GTGCGACAAA GATCCTGGAG TGATGACGTC ATCCTTACAT
    ATTTACCTAC AACTCATACA GGAGAGTCCG GACGCCTATA AGGACCTGAC GGGCAGTTTT
    GTCACCATCC TGAAGCAGGT TGTTGGTGGG AAACTCCCGC TGGATTTTAA CTATCACAGC
    GTTCCTGCGC CTTGGCTTCA GATCCAGCTG CTCCGCATCC TCTCGCTGCT CGGCCGGGAT
    GACCAAAGCA CCAGTGAGCT GATGTATGAA GTTCTGGACG AGTCTCTGCG CAGAGCGGAA
    ATGAACCACA ACATCACCTA CGCAATCCTG TTTGAGTGTG TGAAGGCCGT CTACACCATC
    CACCCTAAAG CAGAGCTTCT GGAGAAAGCA GCGAGATGCA TCGGGAATTT TGTTCTGTCT
    CCAAAGATTA ACCTTAAGTA TCTCGGTTTG AAAGCCTTGA CGTATGTTGT CCAGCATGAT
    GCCAAACTGG CCTTACAGCA TCAGATGACC ATTATTGAGT GTTTGGACCA CTCAGATTTC
    ACTATTAAAA GAGAGACTCT TGAATTGCTG TTCAGGATCA CGAACGCTCA GAATGTGAGT
    GTGATTGTTG AGAAGATGCT GGATTTTCTG CGCAGCTGCA GCGACGAACA CACCATCATC
    CATCTAGCCG GAAAAGTGGC AGAGCTCGCT GAAAAGTTTG CTCCAGACAA CTCCTGGTTT
    ATTCAGACCA TGAATGATGT TTTTTCTATC GGCGGAGATT TGCTGCAGCA GGACATCTCC
    AATAATTTTC TGCGACTGCT GGCGGAGGGA TCAGAGTCTA AAGAGGAGGA TGATCAGCTA
    CGGCTGTACG CTGTCGACTC GTATCTGACA TTATTGAACG GAGACGCTTC TCATCTGCCG
    CAACGCTTCA TTCAGGTCAT GAGCTGGGTT GTTGGTGAGT ATTCTCATCT TCAGGAGTGT
    GTGGATCAGA GCGAGGTGAT TCGGCTCCTG ATGAAGCTGC TGGAGCAGAA GAGTGTGAGC
    TCAGAGACGC GGGTCTGGAT TCTCTCAGCT CTGACCAAGA TTTCAGATGG CCAGACAGAC
    TTAATTCTGG AGCTGGCAGA GAGTATTTCA TCCTCTCAGG ACACGATTCT CCGTCAGCAG
    GCCCACGAGC TGCGGCATCT CAGCCTGGAC TCAAAGCTAC ATGAGTGGGT TTTGCCCCGC
    GGAGCCGCCT GGGACGACAT GGAGGTCGAC TCTTCTTTGT CATTTCTGGA TGGGTTTGTT
    TCTGAAGCTT TGGCTGCAGG AGCCGCACCA TATAAACCTC CTCATCAGAG ACAAGAAGAG
    CTGTGTGAGC AGAGAGCACT GAATCTGGAG CCATACAGTT TTTCTCTGCC TGTGAGTTTG
    TCATCGTGTA GCATCACTGA CAGACACTCT CCTACATTAC TGTCCGTCAG CTCAGGACTT
    TCAGGCAACA GCACTGAGCT CTCTCAGAAA GCAGGTGCCA GCACTCTAAT CCTCGAGGGT
    GTAAAGAGAG TTTGGGGTAA AGATGGGTAC CTTCCACAAA AAGAAACAGC AGTAACAGCC
    TCTCCGGTGG ATGAGCCCAG TGCTGCAGTT CAGCCCGTCG GCCAGCAGGA AGCAGCAGAG
    AGATTAGAGC CACAGCCGGA GCCTTCAACG CAAACCAACG ACAAACAGCA GCTGGCTTCC
    TCTTTGTTTG TTGGTTTGAG TACGCAGAGT TCCACATCTT TGCTGGGAAA ATCTGAAGCT
    CCGCCTCCTC GGTTCAGAAA GAAGCCCCGC CCTCATGGAT CATCATTATC ATCCTCATCC
    TCATCAGGAA CAAGTGAAAA GTCTTCCTCC TCCCGGACCT CACATAACAC AACTGACAGC
    TTCCTGTATG GCAGTTTGCT AGAGGAGGAG CCAGCCGGTT TTGACCACAC CCCCAAATCC
    ATCACAGCCA ATGGTCTTGG CCAAAATAAA GACGTTAAAG AAGACAGACG TACAAACACA
    TCTCAGATAC TGGGCAGTGA TATGGTGATA GTGCGGCCCA ACACAATACA ACACAATCCG
    TCCACGTCTT CAGACCTGAC CACTCTCTTA CCTGCAGACC TTAAAGACCT ACCTCACTCT
    GATGTCATCA GCCTGACCTC TGACCCCAGC CTGAGTGTGT CATCCTGCCG CGTGTTCAGA
    GATGAAGCTC TGCTGCTCGT CATCTTCATT TCTAACAGCA CAGAGACTGA TATAAGAGAT
    GTTGCAGTGC AGCTCAACTG TGACCAGCTG GAGACGGTGA GTTTGCGAGG GAATATCGTG
    GACTGTCTGG AGGGCAGGAG TGTGTCTGTA TGCCATTACG CTCTGCTGAT GGACGACCCT
    CACACTAATG TGTCCTTTAC CGGGAGCGTC TCTTATCAGA TACAACCAGG ACACACAAAC
    ACACTCTCTT TCTCTGGGAC ACTGTCCACA GCGGACTTTA TCAGGCCTTT AGTTGTAACC
    ACAGATGAAT ATGGGAAACT CTGGCTGTCT CTGTCTCATG ACGTCAAGCA AAATCTGAAA
    CTGCTCACAG ATGCCGGTGA CCCTCTCCAA ACAACCCTGA ATGCCCTCAA GGACACCCTA
    CAGCTGCATA TAGTGGACAT CATCGGCTCA GAGGGAATCG TAGCATGTCG TCTGCTCAGC
    GGCGCCCCCT GCCTCCTGCA CTGTCGAGTG AACGGCTCTG CTCTGGCCAT GTGGCTGCGC
    TCTCCACTTC CTGCTCTCCC AGACTGCATC CTGTACCACT GTCAGAGAGC GCTCGCACAA
    CACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_699042.2
    CDS159..3524
    Translation

    Target ORF information:

    RefSeq Version XM_693950.6
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio adaptor-related protein complex 4, epsilon 1 subunit (ap4e1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_693950.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGTGATG TTGTGGAGAA AACTCTCACT GCTCTGCCGA GTTTGTTTGA TTCTCAAACC 
    GGAGCCGCAA AAATCAGCAA CAGACTCAAA CAGCTGATCA AGAGCATCAC AGAGCTGACC
    TCCAAACATG ACGAGGAGAG TCTCATCAAA CAGGAGCTCT CGGCCATGAA GGAGCAGGTT
    TCGGCTCCCA GCACCAGCAT GAAGCAGATG AGAGAGATCA TGGTGAGGTC CATGTACTGT
    GAGATGCTGG GCTACGACGC CTCCTTCAGC TACATCCATG CCATCAAACT GGCCCAGCAG
    GGCGGCGTCA TGGAGAAGAG AGTCGGGTAT CTGGCAGTGT CTCTTTTCCT CAGTGAAGGC
    CATGAGCTGC TGCTGCTGCT AGTCAACACA GTATTAAAGG ATCTTCAGAG CACCAACCTG
    ATTGAAGCCT GCATGGCCTT GACTGTTGTA GCTCAGGTGT TTCCCAAAGA CATGATTCCT
    GCTGTCCTTC CTCTGGTGGA GGACAAGCTT TCACACCCCA AGGAGATCAT TAGGAGGAAA
    GCTGTCCTGG CCTTATATAA GTTTTATCTC ATCGCTCCTA ATCAAGTGCA GCACATTCAT
    GCCAAGTTCC GGAAGGCTCT GTGCGACAAA GATCCTGGAG TGATGACGTC ATCCTTACAT
    ATTTACCTAC AACTCATACA GGAGAGTCCG GACGCCTATA AGGACCTGAC GGGCAGTTTT
    GTCACCATCC TGAAGCAGGT TGTTGGTGGG AAACTCCCGC TGGATTTTAA CTATCACAGC
    GTTCCTGCGC CTTGGCTTCA GATCCAGCTG CTCCGCATCC TCTCGCTGCT CGGCCGGGAT
    GACCAAAGCA CCAGTGAGCT GATGTATGAA GTTCTGGACG AGTCTCTGCG CAGAGCGGAA
    ATGAACCACA ACATCACCTA CGCAATCCTG TTTGAGTGTG TGAAGGCCGT CTACACCATC
    CACCCTAAAG CAGAGCTTCT GGAGAAAGCA GCGAGATGCA TCGGGAATTT TGTTCTGTCT
    CCAAAGATTA ACCTTAAGTA TCTCGGTTTG AAAGCCTTGA CGTATGTTGT CCAGCATGAT
    GCCAAACTGG CCTTACAGCA TCAGATGACC ATTATTGAGT GTTTGGACCA CTCAGATTTC
    ACTATTAAAA GAGAGACTCT TGAATTGCTG TTCAGGATCA CGAACGCTCA GAATGTGAGT
    GTGATTGTTG AGAAGATGCT GGATTTTCTG CGCAGCTGCA GCGACGAACA CACCATCATC
    CATCTAGCCG GAAAAGTGGC AGAGCTCGCT GAAAAGTTTG CTCCAGACAA CTCCTGGTTT
    ATTCAGACCA TGAATGATGT TTTTTCTATC GGCGGAGATT TGCTGCAGCA GGACATCTCC
    AATAATTTTC TGCGACTGCT GGCGGAGGGA TCAGAGTCTA AAGAGGAGGA TGATCAGCTA
    CGGCTGTACG CTGTCGACTC GTATCTGACA TTATTGAACG GAGACGCTTC TCATCTGCCG
    CAACGCTTCA TTCAGGTCAT GAGCTGGGTT GTTGGTGAGT ATTCTCATCT TCAGGAGTGT
    GTGGATCAGA GCGAGGTGAT TCGGCTCCTG ATGAAGCTGC TGGAGCAGAA GAGTGTGAGC
    TCAGAGACGC GGGTCTGGAT TCTCTCAGCT CTGACCAAGA TTTCAGATGG CCAGACAGAC
    TTAATTCTGG AGCTGGCAGA GAGTATTTCA TCCTCTCAGG ACACGATTCT CCGTCAGCAG
    GCCCACGAGC TGCGGCATCT CAGCCTGGAC TCAAAGCTAC ATGAGTGGGT TTTGCCCCGC
    GGAGCCGCCT GGGACGACAT GGAGGTCGAC TCTTCTTTGT CATTTCTGGA TGGGTTTGTT
    TCTGAAGCTT TGGCTGCAGG AGCCGCACCA TATAAACCTC CTCATCAGAG ACAAGAAGAG
    CTGTGTGAGC AGAGAGCACT GAATCTGGAG CCATACAGTT TTTCTCTGCC TGTGAGTTTG
    TCATCGTGTA GCATCACTGA CAGACACTCT CCTACATTAC TGTCCGTCAG CTCAGGACTT
    TCAGGCAACA GCACTGAGCT CTCTCAGAAA GCAGGTGCCA GCACTCTAAT CCTCGAGGGT
    GTAAAGAGAG TTTGGGGTAA AGATGGGTAC CTTCCACAAA AAGAAACAGC AGTAACAGCC
    TCTCCGGTGG ATGAGCCCAG TGCTGCAGTT CAGCCCGTCG GCCAGCAGGA AGCAGCAGAG
    AGATTAGAGC CACAGCCGGA GCCTTCAACG CAAACCAACG ACAAACAGCA GCTGGCTTCC
    TCTTTGTTTG TTGGTTTGAG TACGCAGAGT TCCACATCTT TGCTGGGAAA ATCTGAAGCT
    CCGCCTCCTC GGTTCAGAAA GAAGCCCCGC CCTCATGGAT CATCATTATC ATCCTCATCC
    TCATCAGGAA CAAGTGAAAA GTCTTCCTCC TCCCGGACCT CACATAACAC AACTGACAGC
    TTCCTGTATG GCAGTTTGCT AGAGGAGGAG CCAGCCGGTT TTGACCACAC CCCCAAATCC
    ATCACAGCCA ATGGTCTTGG CCAAAATAAA GACGTTAAAG AAGACAGACG TACAAACACA
    TCTCAGATAC TGGGCAGTGA TATGGTGATA GTGCGGCCCA ACACAATACA ACACAATCCG
    TCCACGTCTT CAGACCTGAC CACTCTCTTA CCTGCAGACC TTAAAGACCT ACCTCACTCT
    GATGTCATCA GCCTGACCTC TGACCCCAGC CTGAGTGTGT CATCCTGCCG CGTGTTCAGA
    GATGAAGCTC TGCTGCTCGT CATCTTCATT TCTAACAGCA CAGAGACTGA TATAAGAGAT
    GTTGCAGTGC AGCTCAACTG TGACCAGCTG GAGACGGTGA GTTTGCGAGG GAATATCGTG
    GACTGTCTGG AGGGCAGGAG TGTGTCTGTA TGCCATTACG CTCTGCTGAT GGACGACCCT
    CACACTAATG TGTCCTTTAC CGGGAGCGTC TCTTATCAGA TACAACCAGG ACACACAAAC
    ACACTCTCTT TCTCTGGGAC ACTGTCCACA GCGGACTTTA TCAGGCCTTT AGTTGTAACC
    ACAGATGAAT ATGGGAAACT CTGGCTGTCT CTGTCTCATG ACGTCAAGCA AAATCTGAAA
    CTGCTCACAG ATGCCGGTGA CCCTCTCCAA ACAACCCTGA ATGCCCTCAA GGACACCCTA
    CAGCTGCATA TAGTGGACAT CATCGGCTCA GAGGGAATCG TAGCATGTCG TCTGCTCAGC
    GGCGCCCCCT GCCTCCTGCA CTGTCGAGTG AACGGCTCTG CTCTGGCCAT GTGGCTGCGC
    TCTCCACTTC CTGCTCTCCC AGACTGCATC CTGTACCACT GTCAGAGAGC GCTCGCACAA
    CACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa29084
    Clone ID Related Accession (Same CDS sequence) XM_693950.6 , XM_693950.7
    Accession Version XM_693950.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3366bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product AP-4 complex subunit epsilon-1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_693950.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGTGATG TTGTGGAGAA AACTCTCACT GCTCTGCCGA GTTTGTTTGA TTCTCAAACC 
    GGAGCCGCAA AAATCAGCAA CAGACTCAAA CAGCTGATCA AGAGCATCAC AGAGCTGACC
    TCCAAACATG ACGAGGAGAG TCTCATCAAA CAGGAGCTCT CGGCCATGAA GGAGCAGGTT
    TCGGCTCCCA GCACCAGCAT GAAGCAGATG AGAGAGATCA TGGTGAGGTC CATGTACTGT
    GAGATGCTGG GCTACGACGC CTCCTTCAGC TACATCCATG CCATCAAACT GGCCCAGCAG
    GGCGGCGTCA TGGAGAAGAG AGTCGGGTAT CTGGCAGTGT CTCTTTTCCT CAGTGAAGGC
    CATGAGCTGC TGCTGCTGCT AGTCAACACA GTATTAAAGG ATCTTCAGAG CACCAACCTG
    ATTGAAGCCT GCATGGCCTT GACTGTTGTA GCTCAGGTGT TTCCCAAAGA CATGATTCCT
    GCTGTCCTTC CTCTGGTGGA GGACAAGCTT TCACACCCCA AGGAGATCAT TAGGAGGAAA
    GCTGTCCTGG CCTTATATAA GTTTTATCTC ATCGCTCCTA ATCAAGTGCA GCACATTCAT
    GCCAAGTTCC GGAAGGCTCT GTGCGACAAA GATCCTGGAG TGATGACGTC ATCCTTACAT
    ATTTACCTAC AACTCATACA GGAGAGTCCG GACGCCTATA AGGACCTGAC GGGCAGTTTT
    GTCACCATCC TGAAGCAGGT TGTTGGTGGG AAACTCCCGC TGGATTTTAA CTATCACAGC
    GTTCCTGCGC CTTGGCTTCA GATCCAGCTG CTCCGCATCC TCTCGCTGCT CGGCCGGGAT
    GACCAAAGCA CCAGTGAGCT GATGTATGAA GTTCTGGACG AGTCTCTGCG CAGAGCGGAA
    ATGAACCACA ACATCACCTA CGCAATCCTG TTTGAGTGTG TGAAGGCCGT CTACACCATC
    CACCCTAAAG CAGAGCTTCT GGAGAAAGCA GCGAGATGCA TCGGGAATTT TGTTCTGTCT
    CCAAAGATTA ACCTTAAGTA TCTCGGTTTG AAAGCCTTGA CGTATGTTGT CCAGCATGAT
    GCCAAACTGG CCTTACAGCA TCAGATGACC ATTATTGAGT GTTTGGACCA CTCAGATTTC
    ACTATTAAAA GAGAGACTCT TGAATTGCTG TTCAGGATCA CGAACGCTCA GAATGTGAGT
    GTGATTGTTG AGAAGATGCT GGATTTTCTG CGCAGCTGCA GCGACGAACA CACCATCATC
    CATCTAGCCG GAAAAGTGGC AGAGCTCGCT GAAAAGTTTG CTCCAGACAA CTCCTGGTTT
    ATTCAGACCA TGAATGATGT TTTTTCTATC GGCGGAGATT TGCTGCAGCA GGACATCTCC
    AATAATTTTC TGCGACTGCT GGCGGAGGGA TCAGAGTCTA AAGAGGAGGA TGATCAGCTA
    CGGCTGTACG CTGTCGACTC GTATCTGACA TTATTGAACG GAGACGCTTC TCATCTGCCG
    CAACGCTTCA TTCAGGTCAT GAGCTGGGTT GTTGGTGAGT ATTCTCATCT TCAGGAGTGT
    GTGGATCAGA GCGAGGTGAT TCGGCTCCTG ATGAAGCTGC TGGAGCAGAA GAGTGTGAGC
    TCAGAGACGC GGGTCTGGAT TCTCTCAGCT CTGACCAAGA TTTCAGATGG CCAGACAGAC
    TTAATTCTGG AGCTGGCAGA GAGTATTTCA TCCTCTCAGG ACACGATTCT CCGTCAGCAG
    GCCCACGAGC TGCGGCATCT CAGCCTGGAC TCAAAGCTAC ATGAGTGGGT TTTGCCCCGC
    GGAGCCGCCT GGGACGACAT GGAGGTCGAC TCTTCTTTGT CATTTCTGGA TGGGTTTGTT
    TCTGAAGCTT TGGCTGCAGG AGCCGCACCA TATAAACCTC CTCATCAGAG ACAAGAAGAG
    CTGTGTGAGC AGAGAGCACT GAATCTGGAG CCATACAGTT TTTCTCTGCC TGTGAGTTTG
    TCATCGTGTA GCATCACTGA CAGACACTCT CCTACATTAC TGTCCGTCAG CTCAGGACTT
    TCAGGCAACA GCACTGAGCT CTCTCAGAAA GCAGGTGCCA GCACTCTAAT CCTCGAGGGT
    GTAAAGAGAG TTTGGGGTAA AGATGGGTAC CTTCCACAAA AAGAAACAGC AGTAACAGCC
    TCTCCGGTGG ATGAGCCCAG TGCTGCAGTT CAGCCCGTCG GCCAGCAGGA AGCAGCAGAG
    AGATTAGAGC CACAGCCGGA GCCTTCAACG CAAACCAACG ACAAACAGCA GCTGGCTTCC
    TCTTTGTTTG TTGGTTTGAG TACGCAGAGT TCCACATCTT TGCTGGGAAA ATCTGAAGCT
    CCGCCTCCTC GGTTCAGAAA GAAGCCCCGC CCTCATGGAT CATCATTATC ATCCTCATCC
    TCATCAGGAA CAAGTGAAAA GTCTTCCTCC TCCCGGACCT CACATAACAC AACTGACAGC
    TTCCTGTATG GCAGTTTGCT AGAGGAGGAG CCAGCCGGTT TTGACCACAC CCCCAAATCC
    ATCACAGCCA ATGGTCTTGG CCAAAATAAA GACGTTAAAG AAGACAGACG TACAAACACA
    TCTCAGATAC TGGGCAGTGA TATGGTGATA GTGCGGCCCA ACACAATACA ACACAATCCG
    TCCACGTCTT CAGACCTGAC CACTCTCTTA CCTGCAGACC TTAAAGACCT ACCTCACTCT
    GATGTCATCA GCCTGACCTC TGACCCCAGC CTGAGTGTGT CATCCTGCCG CGTGTTCAGA
    GATGAAGCTC TGCTGCTCGT CATCTTCATT TCTAACAGCA CAGAGACTGA TATAAGAGAT
    GTTGCAGTGC AGCTCAACTG TGACCAGCTG GAGACGGTGA GTTTGCGAGG GAATATCGTG
    GACTGTCTGG AGGGCAGGAG TGTGTCTGTA TGCCATTACG CTCTGCTGAT GGACGACCCT
    CACACTAATG TGTCCTTTAC CGGGAGCGTC TCTTATCAGA TACAACCAGG ACACACAAAC
    ACACTCTCTT TCTCTGGGAC ACTGTCCACA GCGGACTTTA TCAGGCCTTT AGTTGTAACC
    ACAGATGAAT ATGGGAAACT CTGGCTGTCT CTGTCTCATG ACGTCAAGCA AAATCTGAAA
    CTGCTCACAG ATGCCGGTGA CCCTCTCCAA ACAACCCTGA ATGCCCTCAA GGACACCCTA
    CAGCTGCATA TAGTGGACAT CATCGGCTCA GAGGGAATCG TAGCATGTCG TCTGCTCAGC
    GGCGCCCCCT GCCTCCTGCA CTGTCGAGTG AACGGCTCTG CTCTGGCCAT GTGGCTGCGC
    TCTCCACTTC CTGCTCTCCC AGACTGCATC CTGTACCACT GTCAGAGAGC GCTCGCACAA
    CACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_699042.2
    CDS175..3540
    Translation

    Target ORF information:

    RefSeq Version XM_693950.7
    Organism Danio rerio(zebrafish)
    Definition Danio rerio adaptor-related protein complex 4, epsilon 1 subunit (ap4e1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_693950.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAGTGATG TTGTGGAGAA AACTCTCACT GCTCTGCCGA GTTTGTTTGA TTCTCAAACC 
    GGAGCCGCAA AAATCAGCAA CAGACTCAAA CAGCTGATCA AGAGCATCAC AGAGCTGACC
    TCCAAACATG ACGAGGAGAG TCTCATCAAA CAGGAGCTCT CGGCCATGAA GGAGCAGGTT
    TCGGCTCCCA GCACCAGCAT GAAGCAGATG AGAGAGATCA TGGTGAGGTC CATGTACTGT
    GAGATGCTGG GCTACGACGC CTCCTTCAGC TACATCCATG CCATCAAACT GGCCCAGCAG
    GGCGGCGTCA TGGAGAAGAG AGTCGGGTAT CTGGCAGTGT CTCTTTTCCT CAGTGAAGGC
    CATGAGCTGC TGCTGCTGCT AGTCAACACA GTATTAAAGG ATCTTCAGAG CACCAACCTG
    ATTGAAGCCT GCATGGCCTT GACTGTTGTA GCTCAGGTGT TTCCCAAAGA CATGATTCCT
    GCTGTCCTTC CTCTGGTGGA GGACAAGCTT TCACACCCCA AGGAGATCAT TAGGAGGAAA
    GCTGTCCTGG CCTTATATAA GTTTTATCTC ATCGCTCCTA ATCAAGTGCA GCACATTCAT
    GCCAAGTTCC GGAAGGCTCT GTGCGACAAA GATCCTGGAG TGATGACGTC ATCCTTACAT
    ATTTACCTAC AACTCATACA GGAGAGTCCG GACGCCTATA AGGACCTGAC GGGCAGTTTT
    GTCACCATCC TGAAGCAGGT TGTTGGTGGG AAACTCCCGC TGGATTTTAA CTATCACAGC
    GTTCCTGCGC CTTGGCTTCA GATCCAGCTG CTCCGCATCC TCTCGCTGCT CGGCCGGGAT
    GACCAAAGCA CCAGTGAGCT GATGTATGAA GTTCTGGACG AGTCTCTGCG CAGAGCGGAA
    ATGAACCACA ACATCACCTA CGCAATCCTG TTTGAGTGTG TGAAGGCCGT CTACACCATC
    CACCCTAAAG CAGAGCTTCT GGAGAAAGCA GCGAGATGCA TCGGGAATTT TGTTCTGTCT
    CCAAAGATTA ACCTTAAGTA TCTCGGTTTG AAAGCCTTGA CGTATGTTGT CCAGCATGAT
    GCCAAACTGG CCTTACAGCA TCAGATGACC ATTATTGAGT GTTTGGACCA CTCAGATTTC
    ACTATTAAAA GAGAGACTCT TGAATTGCTG TTCAGGATCA CGAACGCTCA GAATGTGAGT
    GTGATTGTTG AGAAGATGCT GGATTTTCTG CGCAGCTGCA GCGACGAACA CACCATCATC
    CATCTAGCCG GAAAAGTGGC AGAGCTCGCT GAAAAGTTTG CTCCAGACAA CTCCTGGTTT
    ATTCAGACCA TGAATGATGT TTTTTCTATC GGCGGAGATT TGCTGCAGCA GGACATCTCC
    AATAATTTTC TGCGACTGCT GGCGGAGGGA TCAGAGTCTA AAGAGGAGGA TGATCAGCTA
    CGGCTGTACG CTGTCGACTC GTATCTGACA TTATTGAACG GAGACGCTTC TCATCTGCCG
    CAACGCTTCA TTCAGGTCAT GAGCTGGGTT GTTGGTGAGT ATTCTCATCT TCAGGAGTGT
    GTGGATCAGA GCGAGGTGAT TCGGCTCCTG ATGAAGCTGC TGGAGCAGAA GAGTGTGAGC
    TCAGAGACGC GGGTCTGGAT TCTCTCAGCT CTGACCAAGA TTTCAGATGG CCAGACAGAC
    TTAATTCTGG AGCTGGCAGA GAGTATTTCA TCCTCTCAGG ACACGATTCT CCGTCAGCAG
    GCCCACGAGC TGCGGCATCT CAGCCTGGAC TCAAAGCTAC ATGAGTGGGT TTTGCCCCGC
    GGAGCCGCCT GGGACGACAT GGAGGTCGAC TCTTCTTTGT CATTTCTGGA TGGGTTTGTT
    TCTGAAGCTT TGGCTGCAGG AGCCGCACCA TATAAACCTC CTCATCAGAG ACAAGAAGAG
    CTGTGTGAGC AGAGAGCACT GAATCTGGAG CCATACAGTT TTTCTCTGCC TGTGAGTTTG
    TCATCGTGTA GCATCACTGA CAGACACTCT CCTACATTAC TGTCCGTCAG CTCAGGACTT
    TCAGGCAACA GCACTGAGCT CTCTCAGAAA GCAGGTGCCA GCACTCTAAT CCTCGAGGGT
    GTAAAGAGAG TTTGGGGTAA AGATGGGTAC CTTCCACAAA AAGAAACAGC AGTAACAGCC
    TCTCCGGTGG ATGAGCCCAG TGCTGCAGTT CAGCCCGTCG GCCAGCAGGA AGCAGCAGAG
    AGATTAGAGC CACAGCCGGA GCCTTCAACG CAAACCAACG ACAAACAGCA GCTGGCTTCC
    TCTTTGTTTG TTGGTTTGAG TACGCAGAGT TCCACATCTT TGCTGGGAAA ATCTGAAGCT
    CCGCCTCCTC GGTTCAGAAA GAAGCCCCGC CCTCATGGAT CATCATTATC ATCCTCATCC
    TCATCAGGAA CAAGTGAAAA GTCTTCCTCC TCCCGGACCT CACATAACAC AACTGACAGC
    TTCCTGTATG GCAGTTTGCT AGAGGAGGAG CCAGCCGGTT TTGACCACAC CCCCAAATCC
    ATCACAGCCA ATGGTCTTGG CCAAAATAAA GACGTTAAAG AAGACAGACG TACAAACACA
    TCTCAGATAC TGGGCAGTGA TATGGTGATA GTGCGGCCCA ACACAATACA ACACAATCCG
    TCCACGTCTT CAGACCTGAC CACTCTCTTA CCTGCAGACC TTAAAGACCT ACCTCACTCT
    GATGTCATCA GCCTGACCTC TGACCCCAGC CTGAGTGTGT CATCCTGCCG CGTGTTCAGA
    GATGAAGCTC TGCTGCTCGT CATCTTCATT TCTAACAGCA CAGAGACTGA TATAAGAGAT
    GTTGCAGTGC AGCTCAACTG TGACCAGCTG GAGACGGTGA GTTTGCGAGG GAATATCGTG
    GACTGTCTGG AGGGCAGGAG TGTGTCTGTA TGCCATTACG CTCTGCTGAT GGACGACCCT
    CACACTAATG TGTCCTTTAC CGGGAGCGTC TCTTATCAGA TACAACCAGG ACACACAAAC
    ACACTCTCTT TCTCTGGGAC ACTGTCCACA GCGGACTTTA TCAGGCCTTT AGTTGTAACC
    ACAGATGAAT ATGGGAAACT CTGGCTGTCT CTGTCTCATG ACGTCAAGCA AAATCTGAAA
    CTGCTCACAG ATGCCGGTGA CCCTCTCCAA ACAACCCTGA ATGCCCTCAA GGACACCCTA
    CAGCTGCATA TAGTGGACAT CATCGGCTCA GAGGGAATCG TAGCATGTCG TCTGCTCAGC
    GGCGCCCCCT GCCTCCTGCA CTGTCGAGTG AACGGCTCTG CTCTGGCCAT GTGGCTGCGC
    TCTCCACTTC CTGCTCTCCC AGACTGCATC CTGTACCACT GTCAGAGAGC GCTCGCACAA
    CACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.