srgap1a cDNA ORF clone, Danio rerio(zebrafish)

The following srgap1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the srgap1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa145988 XM_021473245.1
Latest version!
Danio rerio SLIT-ROBO Rho GTPase activating protein 1a (srgap1a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $104.30
$149.00
ODa55889 XM_017352032.2
Latest version!
Danio rerio SLIT-ROBO Rho GTPase activating protein 1a (srgap1a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa55889 XM_017352033.2
Latest version!
Danio rerio SLIT-ROBO Rho GTPase activating protein 1a (srgap1a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa55887 XM_017352030.2
Latest version!
Danio rerio SLIT-ROBO Rho GTPase activating protein 1a (srgap1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa55888 XM_017352031.2
Latest version!
Danio rerio SLIT-ROBO Rho GTPase activating protein 1a (srgap1a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
View Old Accession Versions >>
ODa55887 XM_017352030.1 Danio rerio SLIT-ROBO Rho GTPase activating protein 1a (srgap1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa55888 XM_017352031.1 Danio rerio SLIT-ROBO Rho GTPase activating protein 1a (srgap1a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa55889 XM_017352033.1 Danio rerio SLIT-ROBO Rho GTPase activating protein 1a (srgap1a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa55889 XM_017352032.1 Danio rerio SLIT-ROBO Rho GTPase activating protein 1a (srgap1a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa145988
    Clone ID Related Accession (Same CDS sequence) XM_021473245.1
    Accession Version XM_021473245.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 540bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product SLIT-ROBO Rho GTPase-activating protein 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395011.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGTTGAAAA TGTCGAATCC TCCTGTGAAG TTCAAAAAGG ATAAAGAGAT CATTGTAGAG 
    TACGAGACTC AAGTCAAAGA GATTCGAGCT CAACTGGTGG AGCAGCAGAA ATGTCTAGAG
    CAGCAGACCG ACATGAGGGT CCAGCTCCTT CAGGACCTCC AGGATTTTTT CCGCAAGAAG
    TCTGAGATCG AGATGGAGTA TTCACGAAAC CTGGAGAAAC TCGCCGAACG CTTCATGGCC
    AAGACAAGGA GCACCAAGGA CCACCAGCAA TATAAGAAAG ACCAAAATCT GCTGTCCCCG
    GTGAACTGCT GGTACCTGTT GCTGAACCAG GTCCGACGAG AGAGCAAAGA CCACGCGACC
    CTGAGTGACA TTTATCTGAA TAATGTCATC ATGCGCTTCA TGCAGATCAG TGAAGACTCC
    ACACGTCTTA TGAAAAAGAG TAAAGAAATC ACCTTTCAGC TGCAAGAAGA TTTGATGAAA
    GTCCTCAACG AGCTCTACAC GGTAAGTACT GTAACACTTA TCACATTTGT CGAATTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021328920.1
    CDS509..1048
    Translation

    Target ORF information:

    RefSeq Version XM_021473245.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio SLIT-ROBO Rho GTPase activating protein 1a (srgap1a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021473245.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    ATGTTGAAAA TGTCGAATCC TCCTGTGAAG TTCAAAAAGG ATAAAGAGAT CATTGTAGAG 
    TACGAGACTC AAGTCAAAGA GATTCGAGCT CAACTGGTGG AGCAGCAGAA ATGTCTAGAG
    CAGCAGACCG ACATGAGGGT CCAGCTCCTT CAGGACCTCC AGGATTTTTT CCGCAAGAAG
    TCTGAGATCG AGATGGAGTA TTCACGAAAC CTGGAGAAAC TCGCCGAACG CTTCATGGCC
    AAGACAAGGA GCACCAAGGA CCACCAGCAA TATAAGAAAG ACCAAAATCT GCTGTCCCCG
    GTGAACTGCT GGTACCTGTT GCTGAACCAG GTCCGACGAG AGAGCAAAGA CCACGCGACC
    CTGAGTGACA TTTATCTGAA TAATGTCATC ATGCGCTTCA TGCAGATCAG TGAAGACTCC
    ACACGTCTTA TGAAAAAGAG TAAAGAAATC ACCTTTCAGC TGCAAGAAGA TTTGATGAAA
    GTCCTCAACG AGCTCTACAC GGTAAGTACT GTAACACTTA TCACATTTGT CGAATTCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55889
    Clone ID Related Accession (Same CDS sequence) XM_017352033.2 , XM_017352032.1 , XM_017352033.1 , XM_017352032.2
    Accession Version XM_017352033.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2964bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product SLIT-ROBO Rho GTPase-activating protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352033.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTGAAAA TGTCGAATCC TCCTGTGAAG TTCAAAAAGG ATAAAGAGAT CATTGTAGAG 
    TACGAGACTC AAGTCAAAGA GATTCGAGCT CAACTGGTGG AGCAGCAGAA ATGTCTAGAG
    CAGCAGACCG ACATGAGGGT CCAGCTCCTT CAGGACCTCC AGGATTTTTT CCGCAAGAAG
    TCTGAGATCG AGATGGAGTA TTCACGAAAC CTGGAGAAAC TCGCCGAACG CTTCATGGCC
    AAGACAAGGA GCACCAAGGA CCACCAGCAA TATAAGAAAG ACCAGAATCT GCTGTCCCCG
    GTGAACTGCT GGTACCTGTT GCTGAACCAG GTCCGACGAG AGAGCAAAGA CCACGCAACC
    CTGAGTGACA TTTATCTGAA TAATGTCATC ATGAGATTCA TGCAGATCAG TGAAGACTCC
    ACACGTCTTA TGAAAAAGAG TAAAGAAATC ACCTTTCAGC TGCAAGAAGA TTTGATGAAA
    GTCCTCAACG AGCTCTACAC GGTGATGAAG ACCTATCATA TGTATCACAC AGAGAGCATC
    AGTGCTGAGG GTAAACTGAA GGAGGCTGAG AAGCAGGAGG AGAAGCAGAT CGGCCGCGTT
    GGAGATCCAG TTTTCCACAT CCGGCTGGAG GACAGACACC AGAGACGCAG CTCCGTCAAG
    AAGATCGAGA AGATGAAGGA GAAGAGGCAG GCGAAGTATT CGGAGAACAA GCTGAAGTCG
    ATCAAAGCTC GGAACGAATA CCTGCTGACG CTGGAAGCCA CTAATGCTTC AATCTTCAAG
    TATTACATCC ACGACTTGTC TGATTTAATA GACTGTTGTG ATCTGGGATA TCATGCCTCT
    CTGAACCGGG CATTGCGGAC CTACCTCTCA GCCGAATATA ACCTGGAGAC GTCCCGTCAC
    GAGGGCCTGG ACATCATAGA GAATGCAGTG GACAGTCTGG ACCCCCGTAG TGACCGCCAG
    CGCTTCATGG AAATGTACCC GACCGCCTTC TGCCCTCCCG TCAAATTTGA GTTTCAGGCA
    CATATGGGAG ACGAGGTTTG TCAGATCACG GCACAGCCTC CTGTTCAGGC TGAGCTCCTC
    CTGAGGTTCC AGCAGCTTCA ATCCCGCCTC ACCACACTGA AGATCGAGAA CGAAGAGATC
    AAGAAGACGT CTGAGGCGAC ACTTACTACC ATCCAGGACA TGGTGACGGT TGAGGATTAT
    GACGTATCTG AATGTTTCCA TCATAGCCGC TCAACTGAAT CAGTTAAATC TACCGTGTCT
    GAAACCTACC TCAGCAAGCC GAGCATCGCC AAGAGACGCG CCAACCAGCA GGAGACTGAG
    CAGTTCTATT TTATGAAGTT TCGCGAGTAT CTGGAGGGCA GCAATCTCAT CACTAAACTC
    CAGGCAAAGC ATGATCTACT GAAGAGAACC CTCGGTGACG GTTACCGGCC TGAATATGCA
    ACTACAAGGC CTCCCAGTCT TCCTCCTAAA CCGTTTGGCA CACAGAGGGC CAGGCCACGC
    TCTGTATTTA ACGTTAAGCT GTTTAATGGC AATTTGGAGT CTTTTGTTAA GGACTCTGGT
    CAAGCAATAC CTCGAGTTGT TGAGAGCTGT ATTCGATTCA TCAACCTGTA TGGCCTCCAA
    CATCAAGGAA TATTCAGAGT GCCTGGATCT CAAGTTGAAG TCAATGATAT CAAGAATTCA
    TTTGAGAGAG GGAACGATCC ACTGACAGAC GAAGAGAATA ACCACGATAT AAACTCTGTC
    GCTGGCGTTT TGAAACTCTA CTTTCGGGGC TTGGAGAACC CTCTGTTTCC AAAGGAAAGA
    TTCAATGATC TCATCTCCTG TATCAGAATA GAAAATCTGT ATGAAAGAGC GTTGTATATT
    CGAAAGATCC TTCTGACTGC ACCCAGATCA GTGCTTGTGG TCATGCGATA TCTGTTCGCC
    TTCCTCAACC ACTTGTCCCA GTACAGTGAT GAAAACATGA TGGACCCCTA TAATCTGGCC
    ATCTGCTTTG GGCCGACCCT GATGCCCACT CCCGAAACCC AGGACCAGGT GTCCTGCCAG
    GCCCACGTCA ATGAGGTCAT CAAGACCATC ATCATCCACC ACGAAACCAT CTTTCCTGAC
    TCCAAGGAGC TAGACGGCCC CCTGTATGAG AAGTGTATGG CAGGTGGAGA TTATTGTGAG
    AGTCCATACA GTGAACATGG AGCTCTTGAA GAGGTTGATC AGGATGGAGG AGCAGAGACC
    CACACCAGTG AGGATGAGGG AGAGCCCATT GAAGCCATCG CCAAGTTCGA TTACGTGGGC
    CGCTCTGCGC GGGAGCTGTC ATTTAAGAAG GGGGCATCTC TGCTGCTGTA CCAGAGAGCA
    TCCGACGATT GGTGGGAGGG CAGACACAAT GGCATAGATG GACTCGTGCC TCACCAGTAC
    ATTGTTGTTC AGGATATGGA CGACACGTTC TCAGACACTT TGAGTCAGAA AGCGGACAGT
    GAGGCCAGCA GTGGACATGG AGGAGAGGAG AAGTGCTCCA GCAGAGACAT GGGCTCTCCG
    ACAGACAGCA GACTGCCTGA TGCCTACATT AGCAGGCACA GAAAGAGAAG TGACCCTCCA
    ACCCGCAGAC CTCCGGCTCG CCCAACTGAC ACCCACTGCC TCGTCCACCC ATCTCATCAC
    AGCAGCCATA CCAACCCAGA TCTGGGCTCT CCGGTGATGG GCCACTACAG CCCGCGGGAC
    ATGCTGAGGG GCCGAGGACA CATGCCCATG GACAGTCCAG AGAGACGACG GCGAACAGGC
    CATGGCAGCC TGACCAACAT CAGCCGCCAC GAGTCCATTA AGAAGATGGA GAGTCCACCC
    ATCCGCCGGT CCACCTCTTC AGGACAGTAC AGCAGCTTCA ACGAGCCTCA TGTCAAGAGT
    CTGGACCCCG AGAGCATCGC ACAGGTCAGC GGGAGGCGAG CGACGACGCC ACTCACACCA
    TGCGCTGCAG GCCCTGCCCT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207522.1
    CDS875..3838
    Translation

    Target ORF information:

    RefSeq Version XM_017352033.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio SLIT-ROBO Rho GTPase activating protein 1a (srgap1a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352033.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTGAAAA TGTCGAATCC TCCTGTGAAG TTCAAAAAGG ATAAAGAGAT CATTGTAGAG 
    TACGAGACTC AAGTCAAAGA GATTCGAGCT CAACTGGTGG AGCAGCAGAA ATGTCTAGAG
    CAGCAGACCG ACATGAGGGT CCAGCTCCTT CAGGACCTCC AGGATTTTTT CCGCAAGAAG
    TCTGAGATCG AGATGGAGTA TTCACGAAAC CTGGAGAAAC TCGCCGAACG CTTCATGGCC
    AAGACAAGGA GCACCAAGGA CCACCAGCAA TATAAGAAAG ACCAGAATCT GCTGTCCCCG
    GTGAACTGCT GGTACCTGTT GCTGAACCAG GTCCGACGAG AGAGCAAAGA CCACGCAACC
    CTGAGTGACA TTTATCTGAA TAATGTCATC ATGAGATTCA TGCAGATCAG TGAAGACTCC
    ACACGTCTTA TGAAAAAGAG TAAAGAAATC ACCTTTCAGC TGCAAGAAGA TTTGATGAAA
    GTCCTCAACG AGCTCTACAC GGTGATGAAG ACCTATCATA TGTATCACAC AGAGAGCATC
    AGTGCTGAGG GTAAACTGAA GGAGGCTGAG AAGCAGGAGG AGAAGCAGAT CGGCCGCGTT
    GGAGATCCAG TTTTCCACAT CCGGCTGGAG GACAGACACC AGAGACGCAG CTCCGTCAAG
    AAGATCGAGA AGATGAAGGA GAAGAGGCAG GCGAAGTATT CGGAGAACAA GCTGAAGTCG
    ATCAAAGCTC GGAACGAATA CCTGCTGACG CTGGAAGCCA CTAATGCTTC AATCTTCAAG
    TATTACATCC ACGACTTGTC TGATTTAATA GACTGTTGTG ATCTGGGATA TCATGCCTCT
    CTGAACCGGG CATTGCGGAC CTACCTCTCA GCCGAATATA ACCTGGAGAC GTCCCGTCAC
    GAGGGCCTGG ACATCATAGA GAATGCAGTG GACAGTCTGG ACCCCCGTAG TGACCGCCAG
    CGCTTCATGG AAATGTACCC GACCGCCTTC TGCCCTCCCG TCAAATTTGA GTTTCAGGCA
    CATATGGGAG ACGAGGTTTG TCAGATCACG GCACAGCCTC CTGTTCAGGC TGAGCTCCTC
    CTGAGGTTCC AGCAGCTTCA ATCCCGCCTC ACCACACTGA AGATCGAGAA CGAAGAGATC
    AAGAAGACGT CTGAGGCGAC ACTTACTACC ATCCAGGACA TGGTGACGGT TGAGGATTAT
    GACGTATCTG AATGTTTCCA TCATAGCCGC TCAACTGAAT CAGTTAAATC TACCGTGTCT
    GAAACCTACC TCAGCAAGCC GAGCATCGCC AAGAGACGCG CCAACCAGCA GGAGACTGAG
    CAGTTCTATT TTATGAAGTT TCGCGAGTAT CTGGAGGGCA GCAATCTCAT CACTAAACTC
    CAGGCAAAGC ATGATCTACT GAAGAGAACC CTCGGTGACG GTTACCGGCC TGAATATGCA
    ACTACAAGGC CTCCCAGTCT TCCTCCTAAA CCGTTTGGCA CACAGAGGGC CAGGCCACGC
    TCTGTATTTA ACGTTAAGCT GTTTAATGGC AATTTGGAGT CTTTTGTTAA GGACTCTGGT
    CAAGCAATAC CTCGAGTTGT TGAGAGCTGT ATTCGATTCA TCAACCTGTA TGGCCTCCAA
    CATCAAGGAA TATTCAGAGT GCCTGGATCT CAAGTTGAAG TCAATGATAT CAAGAATTCA
    TTTGAGAGAG GGAACGATCC ACTGACAGAC GAAGAGAATA ACCACGATAT AAACTCTGTC
    GCTGGCGTTT TGAAACTCTA CTTTCGGGGC TTGGAGAACC CTCTGTTTCC AAAGGAAAGA
    TTCAATGATC TCATCTCCTG TATCAGAATA GAAAATCTGT ATGAAAGAGC GTTGTATATT
    CGAAAGATCC TTCTGACTGC ACCCAGATCA GTGCTTGTGG TCATGCGATA TCTGTTCGCC
    TTCCTCAACC ACTTGTCCCA GTACAGTGAT GAAAACATGA TGGACCCCTA TAATCTGGCC
    ATCTGCTTTG GGCCGACCCT GATGCCCACT CCCGAAACCC AGGACCAGGT GTCCTGCCAG
    GCCCACGTCA ATGAGGTCAT CAAGACCATC ATCATCCACC ACGAAACCAT CTTTCCTGAC
    TCCAAGGAGC TAGACGGCCC CCTGTATGAG AAGTGTATGG CAGGTGGAGA TTATTGTGAG
    AGTCCATACA GTGAACATGG AGCTCTTGAA GAGGTTGATC AGGATGGAGG AGCAGAGACC
    CACACCAGTG AGGATGAGGG AGAGCCCATT GAAGCCATCG CCAAGTTCGA TTACGTGGGC
    CGCTCTGCGC GGGAGCTGTC ATTTAAGAAG GGGGCATCTC TGCTGCTGTA CCAGAGAGCA
    TCCGACGATT GGTGGGAGGG CAGACACAAT GGCATAGATG GACTCGTGCC TCACCAGTAC
    ATTGTTGTTC AGGATATGGA CGACACGTTC TCAGACACTT TGAGTCAGAA AGCGGACAGT
    GAGGCCAGCA GTGGACATGG AGGAGAGGAG AAGTGCTCCA GCAGAGACAT GGGCTCTCCG
    ACAGACAGCA GACTGCCTGA TGCCTACATT AGCAGGCACA GAAAGAGAAG TGACCCTCCA
    ACCCGCAGAC CTCCGGCTCG CCCAACTGAC ACCCACTGCC TCGTCCACCC ATCTCATCAC
    AGCAGCCATA CCAACCCAGA TCTGGGCTCT CCGGTGATGG GCCACTACAG CCCGCGGGAC
    ATGCTGAGGG GCCGAGGACA CATGCCCATG GACAGTCCAG AGAGACGACG GCGAACAGGC
    CATGGCAGCC TGACCAACAT CAGCCGCCAC GAGTCCATTA AGAAGATGGA GAGTCCACCC
    ATCCGCCGGT CCACCTCTTC AGGACAGTAC AGCAGCTTCA ACGAGCCTCA TGTCAAGAGT
    CTGGACCCCG AGAGCATCGC ACAGGTCAGC GGGAGGCGAG CGACGACGCC ACTCACACCA
    TGCGCTGCAG GCCCTGCCCT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55889
    Clone ID Related Accession (Same CDS sequence) XM_017352033.2 , XM_017352032.1 , XM_017352033.1 , XM_017352032.2
    Accession Version XM_017352032.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2964bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product SLIT-ROBO Rho GTPase-activating protein 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTGAAAA TGTCGAATCC TCCTGTGAAG TTCAAAAAGG ATAAAGAGAT CATTGTAGAG 
    TACGAGACTC AAGTCAAAGA GATTCGAGCT CAACTGGTGG AGCAGCAGAA ATGTCTAGAG
    CAGCAGACCG ACATGAGGGT CCAGCTCCTT CAGGACCTCC AGGATTTTTT CCGCAAGAAG
    TCTGAGATCG AGATGGAGTA TTCACGAAAC CTGGAGAAAC TCGCCGAACG CTTCATGGCC
    AAGACAAGGA GCACCAAGGA CCACCAGCAA TATAAGAAAG ACCAGAATCT GCTGTCCCCG
    GTGAACTGCT GGTACCTGTT GCTGAACCAG GTCCGACGAG AGAGCAAAGA CCACGCAACC
    CTGAGTGACA TTTATCTGAA TAATGTCATC ATGAGATTCA TGCAGATCAG TGAAGACTCC
    ACACGTCTTA TGAAAAAGAG TAAAGAAATC ACCTTTCAGC TGCAAGAAGA TTTGATGAAA
    GTCCTCAACG AGCTCTACAC GGTGATGAAG ACCTATCATA TGTATCACAC AGAGAGCATC
    AGTGCTGAGG GTAAACTGAA GGAGGCTGAG AAGCAGGAGG AGAAGCAGAT CGGCCGCGTT
    GGAGATCCAG TTTTCCACAT CCGGCTGGAG GACAGACACC AGAGACGCAG CTCCGTCAAG
    AAGATCGAGA AGATGAAGGA GAAGAGGCAG GCGAAGTATT CGGAGAACAA GCTGAAGTCG
    ATCAAAGCTC GGAACGAATA CCTGCTGACG CTGGAAGCCA CTAATGCTTC AATCTTCAAG
    TATTACATCC ACGACTTGTC TGATTTAATA GACTGTTGTG ATCTGGGATA TCATGCCTCT
    CTGAACCGGG CATTGCGGAC CTACCTCTCA GCCGAATATA ACCTGGAGAC GTCCCGTCAC
    GAGGGCCTGG ACATCATAGA GAATGCAGTG GACAGTCTGG ACCCCCGTAG TGACCGCCAG
    CGCTTCATGG AAATGTACCC GACCGCCTTC TGCCCTCCCG TCAAATTTGA GTTTCAGGCA
    CATATGGGAG ACGAGGTTTG TCAGATCACG GCACAGCCTC CTGTTCAGGC TGAGCTCCTC
    CTGAGGTTCC AGCAGCTTCA ATCCCGCCTC ACCACACTGA AGATCGAGAA CGAAGAGATC
    AAGAAGACGT CTGAGGCGAC ACTTACTACC ATCCAGGACA TGGTGACGGT TGAGGATTAT
    GACGTATCTG AATGTTTCCA TCATAGCCGC TCAACTGAAT CAGTTAAATC TACCGTGTCT
    GAAACCTACC TCAGCAAGCC GAGCATCGCC AAGAGACGCG CCAACCAGCA GGAGACTGAG
    CAGTTCTATT TTATGAAGTT TCGCGAGTAT CTGGAGGGCA GCAATCTCAT CACTAAACTC
    CAGGCAAAGC ATGATCTACT GAAGAGAACC CTCGGTGACG GTTACCGGCC TGAATATGCA
    ACTACAAGGC CTCCCAGTCT TCCTCCTAAA CCGTTTGGCA CACAGAGGGC CAGGCCACGC
    TCTGTATTTA ACGTTAAGCT GTTTAATGGC AATTTGGAGT CTTTTGTTAA GGACTCTGGT
    CAAGCAATAC CTCGAGTTGT TGAGAGCTGT ATTCGATTCA TCAACCTGTA TGGCCTCCAA
    CATCAAGGAA TATTCAGAGT GCCTGGATCT CAAGTTGAAG TCAATGATAT CAAGAATTCA
    TTTGAGAGAG GGAACGATCC ACTGACAGAC GAAGAGAATA ACCACGATAT AAACTCTGTC
    GCTGGCGTTT TGAAACTCTA CTTTCGGGGC TTGGAGAACC CTCTGTTTCC AAAGGAAAGA
    TTCAATGATC TCATCTCCTG TATCAGAATA GAAAATCTGT ATGAAAGAGC GTTGTATATT
    CGAAAGATCC TTCTGACTGC ACCCAGATCA GTGCTTGTGG TCATGCGATA TCTGTTCGCC
    TTCCTCAACC ACTTGTCCCA GTACAGTGAT GAAAACATGA TGGACCCCTA TAATCTGGCC
    ATCTGCTTTG GGCCGACCCT GATGCCCACT CCCGAAACCC AGGACCAGGT GTCCTGCCAG
    GCCCACGTCA ATGAGGTCAT CAAGACCATC ATCATCCACC ACGAAACCAT CTTTCCTGAC
    TCCAAGGAGC TAGACGGCCC CCTGTATGAG AAGTGTATGG CAGGTGGAGA TTATTGTGAG
    AGTCCATACA GTGAACATGG AGCTCTTGAA GAGGTTGATC AGGATGGAGG AGCAGAGACC
    CACACCAGTG AGGATGAGGG AGAGCCCATT GAAGCCATCG CCAAGTTCGA TTACGTGGGC
    CGCTCTGCGC GGGAGCTGTC ATTTAAGAAG GGGGCATCTC TGCTGCTGTA CCAGAGAGCA
    TCCGACGATT GGTGGGAGGG CAGACACAAT GGCATAGATG GACTCGTGCC TCACCAGTAC
    ATTGTTGTTC AGGATATGGA CGACACGTTC TCAGACACTT TGAGTCAGAA AGCGGACAGT
    GAGGCCAGCA GTGGACATGG AGGAGAGGAG AAGTGCTCCA GCAGAGACAT GGGCTCTCCG
    ACAGACAGCA GACTGCCTGA TGCCTACATT AGCAGGCACA GAAAGAGAAG TGACCCTCCA
    ACCCGCAGAC CTCCGGCTCG CCCAACTGAC ACCCACTGCC TCGTCCACCC ATCTCATCAC
    AGCAGCCATA CCAACCCAGA TCTGGGCTCT CCGGTGATGG GCCACTACAG CCCGCGGGAC
    ATGCTGAGGG GCCGAGGACA CATGCCCATG GACAGTCCAG AGAGACGACG GCGAACAGGC
    CATGGCAGCC TGACCAACAT CAGCCGCCAC GAGTCCATTA AGAAGATGGA GAGTCCACCC
    ATCCGCCGGT CCACCTCTTC AGGACAGTAC AGCAGCTTCA ACGAGCCTCA TGTCAAGAGT
    CTGGACCCCG AGAGCATCGC ACAGGTCAGC GGGAGGCGAG CGACGACGCC ACTCACACCA
    TGCGCTGCAG GCCCTGCCCT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207521.1
    CDS877..3840
    Translation

    Target ORF information:

    RefSeq Version XM_017352032.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio SLIT-ROBO Rho GTPase activating protein 1a (srgap1a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352032.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTGAAAA TGTCGAATCC TCCTGTGAAG TTCAAAAAGG ATAAAGAGAT CATTGTAGAG 
    TACGAGACTC AAGTCAAAGA GATTCGAGCT CAACTGGTGG AGCAGCAGAA ATGTCTAGAG
    CAGCAGACCG ACATGAGGGT CCAGCTCCTT CAGGACCTCC AGGATTTTTT CCGCAAGAAG
    TCTGAGATCG AGATGGAGTA TTCACGAAAC CTGGAGAAAC TCGCCGAACG CTTCATGGCC
    AAGACAAGGA GCACCAAGGA CCACCAGCAA TATAAGAAAG ACCAGAATCT GCTGTCCCCG
    GTGAACTGCT GGTACCTGTT GCTGAACCAG GTCCGACGAG AGAGCAAAGA CCACGCAACC
    CTGAGTGACA TTTATCTGAA TAATGTCATC ATGAGATTCA TGCAGATCAG TGAAGACTCC
    ACACGTCTTA TGAAAAAGAG TAAAGAAATC ACCTTTCAGC TGCAAGAAGA TTTGATGAAA
    GTCCTCAACG AGCTCTACAC GGTGATGAAG ACCTATCATA TGTATCACAC AGAGAGCATC
    AGTGCTGAGG GTAAACTGAA GGAGGCTGAG AAGCAGGAGG AGAAGCAGAT CGGCCGCGTT
    GGAGATCCAG TTTTCCACAT CCGGCTGGAG GACAGACACC AGAGACGCAG CTCCGTCAAG
    AAGATCGAGA AGATGAAGGA GAAGAGGCAG GCGAAGTATT CGGAGAACAA GCTGAAGTCG
    ATCAAAGCTC GGAACGAATA CCTGCTGACG CTGGAAGCCA CTAATGCTTC AATCTTCAAG
    TATTACATCC ACGACTTGTC TGATTTAATA GACTGTTGTG ATCTGGGATA TCATGCCTCT
    CTGAACCGGG CATTGCGGAC CTACCTCTCA GCCGAATATA ACCTGGAGAC GTCCCGTCAC
    GAGGGCCTGG ACATCATAGA GAATGCAGTG GACAGTCTGG ACCCCCGTAG TGACCGCCAG
    CGCTTCATGG AAATGTACCC GACCGCCTTC TGCCCTCCCG TCAAATTTGA GTTTCAGGCA
    CATATGGGAG ACGAGGTTTG TCAGATCACG GCACAGCCTC CTGTTCAGGC TGAGCTCCTC
    CTGAGGTTCC AGCAGCTTCA ATCCCGCCTC ACCACACTGA AGATCGAGAA CGAAGAGATC
    AAGAAGACGT CTGAGGCGAC ACTTACTACC ATCCAGGACA TGGTGACGGT TGAGGATTAT
    GACGTATCTG AATGTTTCCA TCATAGCCGC TCAACTGAAT CAGTTAAATC TACCGTGTCT
    GAAACCTACC TCAGCAAGCC GAGCATCGCC AAGAGACGCG CCAACCAGCA GGAGACTGAG
    CAGTTCTATT TTATGAAGTT TCGCGAGTAT CTGGAGGGCA GCAATCTCAT CACTAAACTC
    CAGGCAAAGC ATGATCTACT GAAGAGAACC CTCGGTGACG GTTACCGGCC TGAATATGCA
    ACTACAAGGC CTCCCAGTCT TCCTCCTAAA CCGTTTGGCA CACAGAGGGC CAGGCCACGC
    TCTGTATTTA ACGTTAAGCT GTTTAATGGC AATTTGGAGT CTTTTGTTAA GGACTCTGGT
    CAAGCAATAC CTCGAGTTGT TGAGAGCTGT ATTCGATTCA TCAACCTGTA TGGCCTCCAA
    CATCAAGGAA TATTCAGAGT GCCTGGATCT CAAGTTGAAG TCAATGATAT CAAGAATTCA
    TTTGAGAGAG GGAACGATCC ACTGACAGAC GAAGAGAATA ACCACGATAT AAACTCTGTC
    GCTGGCGTTT TGAAACTCTA CTTTCGGGGC TTGGAGAACC CTCTGTTTCC AAAGGAAAGA
    TTCAATGATC TCATCTCCTG TATCAGAATA GAAAATCTGT ATGAAAGAGC GTTGTATATT
    CGAAAGATCC TTCTGACTGC ACCCAGATCA GTGCTTGTGG TCATGCGATA TCTGTTCGCC
    TTCCTCAACC ACTTGTCCCA GTACAGTGAT GAAAACATGA TGGACCCCTA TAATCTGGCC
    ATCTGCTTTG GGCCGACCCT GATGCCCACT CCCGAAACCC AGGACCAGGT GTCCTGCCAG
    GCCCACGTCA ATGAGGTCAT CAAGACCATC ATCATCCACC ACGAAACCAT CTTTCCTGAC
    TCCAAGGAGC TAGACGGCCC CCTGTATGAG AAGTGTATGG CAGGTGGAGA TTATTGTGAG
    AGTCCATACA GTGAACATGG AGCTCTTGAA GAGGTTGATC AGGATGGAGG AGCAGAGACC
    CACACCAGTG AGGATGAGGG AGAGCCCATT GAAGCCATCG CCAAGTTCGA TTACGTGGGC
    CGCTCTGCGC GGGAGCTGTC ATTTAAGAAG GGGGCATCTC TGCTGCTGTA CCAGAGAGCA
    TCCGACGATT GGTGGGAGGG CAGACACAAT GGCATAGATG GACTCGTGCC TCACCAGTAC
    ATTGTTGTTC AGGATATGGA CGACACGTTC TCAGACACTT TGAGTCAGAA AGCGGACAGT
    GAGGCCAGCA GTGGACATGG AGGAGAGGAG AAGTGCTCCA GCAGAGACAT GGGCTCTCCG
    ACAGACAGCA GACTGCCTGA TGCCTACATT AGCAGGCACA GAAAGAGAAG TGACCCTCCA
    ACCCGCAGAC CTCCGGCTCG CCCAACTGAC ACCCACTGCC TCGTCCACCC ATCTCATCAC
    AGCAGCCATA CCAACCCAGA TCTGGGCTCT CCGGTGATGG GCCACTACAG CCCGCGGGAC
    ATGCTGAGGG GCCGAGGACA CATGCCCATG GACAGTCCAG AGAGACGACG GCGAACAGGC
    CATGGCAGCC TGACCAACAT CAGCCGCCAC GAGTCCATTA AGAAGATGGA GAGTCCACCC
    ATCCGCCGGT CCACCTCTTC AGGACAGTAC AGCAGCTTCA ACGAGCCTCA TGTCAAGAGT
    CTGGACCCCG AGAGCATCGC ACAGGTCAGC GGGAGGCGAG CGACGACGCC ACTCACACCA
    TGCGCTGCAG GCCCTGCCCT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55889
    Clone ID Related Accession (Same CDS sequence) XM_017352033.2 , XM_017352032.1 , XM_017352033.1 , XM_017352032.2
    Accession Version XM_017352033.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2964bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product SLIT-ROBO Rho GTPase-activating protein 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTGAAAA TGTCGAATCC TCCTGTGAAG TTCAAAAAGG ATAAAGAGAT CATTGTAGAG 
    TACGAGACTC AAGTCAAAGA GATTCGAGCT CAACTGGTGG AGCAGCAGAA ATGTCTAGAG
    CAGCAGACCG ACATGAGGGT CCAGCTCCTT CAGGACCTCC AGGATTTTTT CCGCAAGAAG
    TCTGAGATCG AGATGGAGTA TTCACGAAAC CTGGAGAAAC TCGCCGAACG CTTCATGGCC
    AAGACAAGGA GCACCAAGGA CCACCAGCAA TATAAGAAAG ACCAGAATCT GCTGTCCCCG
    GTGAACTGCT GGTACCTGTT GCTGAACCAG GTCCGACGAG AGAGCAAAGA CCACGCAACC
    CTGAGTGACA TTTATCTGAA TAATGTCATC ATGAGATTCA TGCAGATCAG TGAAGACTCC
    ACACGTCTTA TGAAAAAGAG TAAAGAAATC ACCTTTCAGC TGCAAGAAGA TTTGATGAAA
    GTCCTCAACG AGCTCTACAC GGTGATGAAG ACCTATCATA TGTATCACAC AGAGAGCATC
    AGTGCTGAGG GTAAACTGAA GGAGGCTGAG AAGCAGGAGG AGAAGCAGAT CGGCCGCGTT
    GGAGATCCAG TTTTCCACAT CCGGCTGGAG GACAGACACC AGAGACGCAG CTCCGTCAAG
    AAGATCGAGA AGATGAAGGA GAAGAGGCAG GCGAAGTATT CGGAGAACAA GCTGAAGTCG
    ATCAAAGCTC GGAACGAATA CCTGCTGACG CTGGAAGCCA CTAATGCTTC AATCTTCAAG
    TATTACATCC ACGACTTGTC TGATTTAATA GACTGTTGTG ATCTGGGATA TCATGCCTCT
    CTGAACCGGG CATTGCGGAC CTACCTCTCA GCCGAATATA ACCTGGAGAC GTCCCGTCAC
    GAGGGCCTGG ACATCATAGA GAATGCAGTG GACAGTCTGG ACCCCCGTAG TGACCGCCAG
    CGCTTCATGG AAATGTACCC GACCGCCTTC TGCCCTCCCG TCAAATTTGA GTTTCAGGCA
    CATATGGGAG ACGAGGTTTG TCAGATCACG GCACAGCCTC CTGTTCAGGC TGAGCTCCTC
    CTGAGGTTCC AGCAGCTTCA ATCCCGCCTC ACCACACTGA AGATCGAGAA CGAAGAGATC
    AAGAAGACGT CTGAGGCGAC ACTTACTACC ATCCAGGACA TGGTGACGGT TGAGGATTAT
    GACGTATCTG AATGTTTCCA TCATAGCCGC TCAACTGAAT CAGTTAAATC TACCGTGTCT
    GAAACCTACC TCAGCAAGCC GAGCATCGCC AAGAGACGCG CCAACCAGCA GGAGACTGAG
    CAGTTCTATT TTATGAAGTT TCGCGAGTAT CTGGAGGGCA GCAATCTCAT CACTAAACTC
    CAGGCAAAGC ATGATCTACT GAAGAGAACC CTCGGTGACG GTTACCGGCC TGAATATGCA
    ACTACAAGGC CTCCCAGTCT TCCTCCTAAA CCGTTTGGCA CACAGAGGGC CAGGCCACGC
    TCTGTATTTA ACGTTAAGCT GTTTAATGGC AATTTGGAGT CTTTTGTTAA GGACTCTGGT
    CAAGCAATAC CTCGAGTTGT TGAGAGCTGT ATTCGATTCA TCAACCTGTA TGGCCTCCAA
    CATCAAGGAA TATTCAGAGT GCCTGGATCT CAAGTTGAAG TCAATGATAT CAAGAATTCA
    TTTGAGAGAG GGAACGATCC ACTGACAGAC GAAGAGAATA ACCACGATAT AAACTCTGTC
    GCTGGCGTTT TGAAACTCTA CTTTCGGGGC TTGGAGAACC CTCTGTTTCC AAAGGAAAGA
    TTCAATGATC TCATCTCCTG TATCAGAATA GAAAATCTGT ATGAAAGAGC GTTGTATATT
    CGAAAGATCC TTCTGACTGC ACCCAGATCA GTGCTTGTGG TCATGCGATA TCTGTTCGCC
    TTCCTCAACC ACTTGTCCCA GTACAGTGAT GAAAACATGA TGGACCCCTA TAATCTGGCC
    ATCTGCTTTG GGCCGACCCT GATGCCCACT CCCGAAACCC AGGACCAGGT GTCCTGCCAG
    GCCCACGTCA ATGAGGTCAT CAAGACCATC ATCATCCACC ACGAAACCAT CTTTCCTGAC
    TCCAAGGAGC TAGACGGCCC CCTGTATGAG AAGTGTATGG CAGGTGGAGA TTATTGTGAG
    AGTCCATACA GTGAACATGG AGCTCTTGAA GAGGTTGATC AGGATGGAGG AGCAGAGACC
    CACACCAGTG AGGATGAGGG AGAGCCCATT GAAGCCATCG CCAAGTTCGA TTACGTGGGC
    CGCTCTGCGC GGGAGCTGTC ATTTAAGAAG GGGGCATCTC TGCTGCTGTA CCAGAGAGCA
    TCCGACGATT GGTGGGAGGG CAGACACAAT GGCATAGATG GACTCGTGCC TCACCAGTAC
    ATTGTTGTTC AGGATATGGA CGACACGTTC TCAGACACTT TGAGTCAGAA AGCGGACAGT
    GAGGCCAGCA GTGGACATGG AGGAGAGGAG AAGTGCTCCA GCAGAGACAT GGGCTCTCCG
    ACAGACAGCA GACTGCCTGA TGCCTACATT AGCAGGCACA GAAAGAGAAG TGACCCTCCA
    ACCCGCAGAC CTCCGGCTCG CCCAACTGAC ACCCACTGCC TCGTCCACCC ATCTCATCAC
    AGCAGCCATA CCAACCCAGA TCTGGGCTCT CCGGTGATGG GCCACTACAG CCCGCGGGAC
    ATGCTGAGGG GCCGAGGACA CATGCCCATG GACAGTCCAG AGAGACGACG GCGAACAGGC
    CATGGCAGCC TGACCAACAT CAGCCGCCAC GAGTCCATTA AGAAGATGGA GAGTCCACCC
    ATCCGCCGGT CCACCTCTTC AGGACAGTAC AGCAGCTTCA ACGAGCCTCA TGTCAAGAGT
    CTGGACCCCG AGAGCATCGC ACAGGTCAGC GGGAGGCGAG CGACGACGCC ACTCACACCA
    TGCGCTGCAG GCCCTGCCCT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207522.1
    CDS877..3840
    Translation

    Target ORF information:

    RefSeq Version XM_017352033.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio SLIT-ROBO Rho GTPase activating protein 1a (srgap1a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352033.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTGAAAA TGTCGAATCC TCCTGTGAAG TTCAAAAAGG ATAAAGAGAT CATTGTAGAG 
    TACGAGACTC AAGTCAAAGA GATTCGAGCT CAACTGGTGG AGCAGCAGAA ATGTCTAGAG
    CAGCAGACCG ACATGAGGGT CCAGCTCCTT CAGGACCTCC AGGATTTTTT CCGCAAGAAG
    TCTGAGATCG AGATGGAGTA TTCACGAAAC CTGGAGAAAC TCGCCGAACG CTTCATGGCC
    AAGACAAGGA GCACCAAGGA CCACCAGCAA TATAAGAAAG ACCAGAATCT GCTGTCCCCG
    GTGAACTGCT GGTACCTGTT GCTGAACCAG GTCCGACGAG AGAGCAAAGA CCACGCAACC
    CTGAGTGACA TTTATCTGAA TAATGTCATC ATGAGATTCA TGCAGATCAG TGAAGACTCC
    ACACGTCTTA TGAAAAAGAG TAAAGAAATC ACCTTTCAGC TGCAAGAAGA TTTGATGAAA
    GTCCTCAACG AGCTCTACAC GGTGATGAAG ACCTATCATA TGTATCACAC AGAGAGCATC
    AGTGCTGAGG GTAAACTGAA GGAGGCTGAG AAGCAGGAGG AGAAGCAGAT CGGCCGCGTT
    GGAGATCCAG TTTTCCACAT CCGGCTGGAG GACAGACACC AGAGACGCAG CTCCGTCAAG
    AAGATCGAGA AGATGAAGGA GAAGAGGCAG GCGAAGTATT CGGAGAACAA GCTGAAGTCG
    ATCAAAGCTC GGAACGAATA CCTGCTGACG CTGGAAGCCA CTAATGCTTC AATCTTCAAG
    TATTACATCC ACGACTTGTC TGATTTAATA GACTGTTGTG ATCTGGGATA TCATGCCTCT
    CTGAACCGGG CATTGCGGAC CTACCTCTCA GCCGAATATA ACCTGGAGAC GTCCCGTCAC
    GAGGGCCTGG ACATCATAGA GAATGCAGTG GACAGTCTGG ACCCCCGTAG TGACCGCCAG
    CGCTTCATGG AAATGTACCC GACCGCCTTC TGCCCTCCCG TCAAATTTGA GTTTCAGGCA
    CATATGGGAG ACGAGGTTTG TCAGATCACG GCACAGCCTC CTGTTCAGGC TGAGCTCCTC
    CTGAGGTTCC AGCAGCTTCA ATCCCGCCTC ACCACACTGA AGATCGAGAA CGAAGAGATC
    AAGAAGACGT CTGAGGCGAC ACTTACTACC ATCCAGGACA TGGTGACGGT TGAGGATTAT
    GACGTATCTG AATGTTTCCA TCATAGCCGC TCAACTGAAT CAGTTAAATC TACCGTGTCT
    GAAACCTACC TCAGCAAGCC GAGCATCGCC AAGAGACGCG CCAACCAGCA GGAGACTGAG
    CAGTTCTATT TTATGAAGTT TCGCGAGTAT CTGGAGGGCA GCAATCTCAT CACTAAACTC
    CAGGCAAAGC ATGATCTACT GAAGAGAACC CTCGGTGACG GTTACCGGCC TGAATATGCA
    ACTACAAGGC CTCCCAGTCT TCCTCCTAAA CCGTTTGGCA CACAGAGGGC CAGGCCACGC
    TCTGTATTTA ACGTTAAGCT GTTTAATGGC AATTTGGAGT CTTTTGTTAA GGACTCTGGT
    CAAGCAATAC CTCGAGTTGT TGAGAGCTGT ATTCGATTCA TCAACCTGTA TGGCCTCCAA
    CATCAAGGAA TATTCAGAGT GCCTGGATCT CAAGTTGAAG TCAATGATAT CAAGAATTCA
    TTTGAGAGAG GGAACGATCC ACTGACAGAC GAAGAGAATA ACCACGATAT AAACTCTGTC
    GCTGGCGTTT TGAAACTCTA CTTTCGGGGC TTGGAGAACC CTCTGTTTCC AAAGGAAAGA
    TTCAATGATC TCATCTCCTG TATCAGAATA GAAAATCTGT ATGAAAGAGC GTTGTATATT
    CGAAAGATCC TTCTGACTGC ACCCAGATCA GTGCTTGTGG TCATGCGATA TCTGTTCGCC
    TTCCTCAACC ACTTGTCCCA GTACAGTGAT GAAAACATGA TGGACCCCTA TAATCTGGCC
    ATCTGCTTTG GGCCGACCCT GATGCCCACT CCCGAAACCC AGGACCAGGT GTCCTGCCAG
    GCCCACGTCA ATGAGGTCAT CAAGACCATC ATCATCCACC ACGAAACCAT CTTTCCTGAC
    TCCAAGGAGC TAGACGGCCC CCTGTATGAG AAGTGTATGG CAGGTGGAGA TTATTGTGAG
    AGTCCATACA GTGAACATGG AGCTCTTGAA GAGGTTGATC AGGATGGAGG AGCAGAGACC
    CACACCAGTG AGGATGAGGG AGAGCCCATT GAAGCCATCG CCAAGTTCGA TTACGTGGGC
    CGCTCTGCGC GGGAGCTGTC ATTTAAGAAG GGGGCATCTC TGCTGCTGTA CCAGAGAGCA
    TCCGACGATT GGTGGGAGGG CAGACACAAT GGCATAGATG GACTCGTGCC TCACCAGTAC
    ATTGTTGTTC AGGATATGGA CGACACGTTC TCAGACACTT TGAGTCAGAA AGCGGACAGT
    GAGGCCAGCA GTGGACATGG AGGAGAGGAG AAGTGCTCCA GCAGAGACAT GGGCTCTCCG
    ACAGACAGCA GACTGCCTGA TGCCTACATT AGCAGGCACA GAAAGAGAAG TGACCCTCCA
    ACCCGCAGAC CTCCGGCTCG CCCAACTGAC ACCCACTGCC TCGTCCACCC ATCTCATCAC
    AGCAGCCATA CCAACCCAGA TCTGGGCTCT CCGGTGATGG GCCACTACAG CCCGCGGGAC
    ATGCTGAGGG GCCGAGGACA CATGCCCATG GACAGTCCAG AGAGACGACG GCGAACAGGC
    CATGGCAGCC TGACCAACAT CAGCCGCCAC GAGTCCATTA AGAAGATGGA GAGTCCACCC
    ATCCGCCGGT CCACCTCTTC AGGACAGTAC AGCAGCTTCA ACGAGCCTCA TGTCAAGAGT
    CTGGACCCCG AGAGCATCGC ACAGGTCAGC GGGAGGCGAG CGACGACGCC ACTCACACCA
    TGCGCTGCAG GCCCTGCCCT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55889
    Clone ID Related Accession (Same CDS sequence) XM_017352033.2 , XM_017352032.1 , XM_017352033.1 , XM_017352032.2
    Accession Version XM_017352032.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2964bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product SLIT-ROBO Rho GTPase-activating protein 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352032.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTGAAAA TGTCGAATCC TCCTGTGAAG TTCAAAAAGG ATAAAGAGAT CATTGTAGAG 
    TACGAGACTC AAGTCAAAGA GATTCGAGCT CAACTGGTGG AGCAGCAGAA ATGTCTAGAG
    CAGCAGACCG ACATGAGGGT CCAGCTCCTT CAGGACCTCC AGGATTTTTT CCGCAAGAAG
    TCTGAGATCG AGATGGAGTA TTCACGAAAC CTGGAGAAAC TCGCCGAACG CTTCATGGCC
    AAGACAAGGA GCACCAAGGA CCACCAGCAA TATAAGAAAG ACCAGAATCT GCTGTCCCCG
    GTGAACTGCT GGTACCTGTT GCTGAACCAG GTCCGACGAG AGAGCAAAGA CCACGCAACC
    CTGAGTGACA TTTATCTGAA TAATGTCATC ATGAGATTCA TGCAGATCAG TGAAGACTCC
    ACACGTCTTA TGAAAAAGAG TAAAGAAATC ACCTTTCAGC TGCAAGAAGA TTTGATGAAA
    GTCCTCAACG AGCTCTACAC GGTGATGAAG ACCTATCATA TGTATCACAC AGAGAGCATC
    AGTGCTGAGG GTAAACTGAA GGAGGCTGAG AAGCAGGAGG AGAAGCAGAT CGGCCGCGTT
    GGAGATCCAG TTTTCCACAT CCGGCTGGAG GACAGACACC AGAGACGCAG CTCCGTCAAG
    AAGATCGAGA AGATGAAGGA GAAGAGGCAG GCGAAGTATT CGGAGAACAA GCTGAAGTCG
    ATCAAAGCTC GGAACGAATA CCTGCTGACG CTGGAAGCCA CTAATGCTTC AATCTTCAAG
    TATTACATCC ACGACTTGTC TGATTTAATA GACTGTTGTG ATCTGGGATA TCATGCCTCT
    CTGAACCGGG CATTGCGGAC CTACCTCTCA GCCGAATATA ACCTGGAGAC GTCCCGTCAC
    GAGGGCCTGG ACATCATAGA GAATGCAGTG GACAGTCTGG ACCCCCGTAG TGACCGCCAG
    CGCTTCATGG AAATGTACCC GACCGCCTTC TGCCCTCCCG TCAAATTTGA GTTTCAGGCA
    CATATGGGAG ACGAGGTTTG TCAGATCACG GCACAGCCTC CTGTTCAGGC TGAGCTCCTC
    CTGAGGTTCC AGCAGCTTCA ATCCCGCCTC ACCACACTGA AGATCGAGAA CGAAGAGATC
    AAGAAGACGT CTGAGGCGAC ACTTACTACC ATCCAGGACA TGGTGACGGT TGAGGATTAT
    GACGTATCTG AATGTTTCCA TCATAGCCGC TCAACTGAAT CAGTTAAATC TACCGTGTCT
    GAAACCTACC TCAGCAAGCC GAGCATCGCC AAGAGACGCG CCAACCAGCA GGAGACTGAG
    CAGTTCTATT TTATGAAGTT TCGCGAGTAT CTGGAGGGCA GCAATCTCAT CACTAAACTC
    CAGGCAAAGC ATGATCTACT GAAGAGAACC CTCGGTGACG GTTACCGGCC TGAATATGCA
    ACTACAAGGC CTCCCAGTCT TCCTCCTAAA CCGTTTGGCA CACAGAGGGC CAGGCCACGC
    TCTGTATTTA ACGTTAAGCT GTTTAATGGC AATTTGGAGT CTTTTGTTAA GGACTCTGGT
    CAAGCAATAC CTCGAGTTGT TGAGAGCTGT ATTCGATTCA TCAACCTGTA TGGCCTCCAA
    CATCAAGGAA TATTCAGAGT GCCTGGATCT CAAGTTGAAG TCAATGATAT CAAGAATTCA
    TTTGAGAGAG GGAACGATCC ACTGACAGAC GAAGAGAATA ACCACGATAT AAACTCTGTC
    GCTGGCGTTT TGAAACTCTA CTTTCGGGGC TTGGAGAACC CTCTGTTTCC AAAGGAAAGA
    TTCAATGATC TCATCTCCTG TATCAGAATA GAAAATCTGT ATGAAAGAGC GTTGTATATT
    CGAAAGATCC TTCTGACTGC ACCCAGATCA GTGCTTGTGG TCATGCGATA TCTGTTCGCC
    TTCCTCAACC ACTTGTCCCA GTACAGTGAT GAAAACATGA TGGACCCCTA TAATCTGGCC
    ATCTGCTTTG GGCCGACCCT GATGCCCACT CCCGAAACCC AGGACCAGGT GTCCTGCCAG
    GCCCACGTCA ATGAGGTCAT CAAGACCATC ATCATCCACC ACGAAACCAT CTTTCCTGAC
    TCCAAGGAGC TAGACGGCCC CCTGTATGAG AAGTGTATGG CAGGTGGAGA TTATTGTGAG
    AGTCCATACA GTGAACATGG AGCTCTTGAA GAGGTTGATC AGGATGGAGG AGCAGAGACC
    CACACCAGTG AGGATGAGGG AGAGCCCATT GAAGCCATCG CCAAGTTCGA TTACGTGGGC
    CGCTCTGCGC GGGAGCTGTC ATTTAAGAAG GGGGCATCTC TGCTGCTGTA CCAGAGAGCA
    TCCGACGATT GGTGGGAGGG CAGACACAAT GGCATAGATG GACTCGTGCC TCACCAGTAC
    ATTGTTGTTC AGGATATGGA CGACACGTTC TCAGACACTT TGAGTCAGAA AGCGGACAGT
    GAGGCCAGCA GTGGACATGG AGGAGAGGAG AAGTGCTCCA GCAGAGACAT GGGCTCTCCG
    ACAGACAGCA GACTGCCTGA TGCCTACATT AGCAGGCACA GAAAGAGAAG TGACCCTCCA
    ACCCGCAGAC CTCCGGCTCG CCCAACTGAC ACCCACTGCC TCGTCCACCC ATCTCATCAC
    AGCAGCCATA CCAACCCAGA TCTGGGCTCT CCGGTGATGG GCCACTACAG CCCGCGGGAC
    ATGCTGAGGG GCCGAGGACA CATGCCCATG GACAGTCCAG AGAGACGACG GCGAACAGGC
    CATGGCAGCC TGACCAACAT CAGCCGCCAC GAGTCCATTA AGAAGATGGA GAGTCCACCC
    ATCCGCCGGT CCACCTCTTC AGGACAGTAC AGCAGCTTCA ACGAGCCTCA TGTCAAGAGT
    CTGGACCCCG AGAGCATCGC ACAGGTCAGC GGGAGGCGAG CGACGACGCC ACTCACACCA
    TGCGCTGCAG GCCCTGCCCT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207521.1
    CDS875..3838
    Translation

    Target ORF information:

    RefSeq Version XM_017352032.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio SLIT-ROBO Rho GTPase activating protein 1a (srgap1a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352032.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGTTGAAAA TGTCGAATCC TCCTGTGAAG TTCAAAAAGG ATAAAGAGAT CATTGTAGAG 
    TACGAGACTC AAGTCAAAGA GATTCGAGCT CAACTGGTGG AGCAGCAGAA ATGTCTAGAG
    CAGCAGACCG ACATGAGGGT CCAGCTCCTT CAGGACCTCC AGGATTTTTT CCGCAAGAAG
    TCTGAGATCG AGATGGAGTA TTCACGAAAC CTGGAGAAAC TCGCCGAACG CTTCATGGCC
    AAGACAAGGA GCACCAAGGA CCACCAGCAA TATAAGAAAG ACCAGAATCT GCTGTCCCCG
    GTGAACTGCT GGTACCTGTT GCTGAACCAG GTCCGACGAG AGAGCAAAGA CCACGCAACC
    CTGAGTGACA TTTATCTGAA TAATGTCATC ATGAGATTCA TGCAGATCAG TGAAGACTCC
    ACACGTCTTA TGAAAAAGAG TAAAGAAATC ACCTTTCAGC TGCAAGAAGA TTTGATGAAA
    GTCCTCAACG AGCTCTACAC GGTGATGAAG ACCTATCATA TGTATCACAC AGAGAGCATC
    AGTGCTGAGG GTAAACTGAA GGAGGCTGAG AAGCAGGAGG AGAAGCAGAT CGGCCGCGTT
    GGAGATCCAG TTTTCCACAT CCGGCTGGAG GACAGACACC AGAGACGCAG CTCCGTCAAG
    AAGATCGAGA AGATGAAGGA GAAGAGGCAG GCGAAGTATT CGGAGAACAA GCTGAAGTCG
    ATCAAAGCTC GGAACGAATA CCTGCTGACG CTGGAAGCCA CTAATGCTTC AATCTTCAAG
    TATTACATCC ACGACTTGTC TGATTTAATA GACTGTTGTG ATCTGGGATA TCATGCCTCT
    CTGAACCGGG CATTGCGGAC CTACCTCTCA GCCGAATATA ACCTGGAGAC GTCCCGTCAC
    GAGGGCCTGG ACATCATAGA GAATGCAGTG GACAGTCTGG ACCCCCGTAG TGACCGCCAG
    CGCTTCATGG AAATGTACCC GACCGCCTTC TGCCCTCCCG TCAAATTTGA GTTTCAGGCA
    CATATGGGAG ACGAGGTTTG TCAGATCACG GCACAGCCTC CTGTTCAGGC TGAGCTCCTC
    CTGAGGTTCC AGCAGCTTCA ATCCCGCCTC ACCACACTGA AGATCGAGAA CGAAGAGATC
    AAGAAGACGT CTGAGGCGAC ACTTACTACC ATCCAGGACA TGGTGACGGT TGAGGATTAT
    GACGTATCTG AATGTTTCCA TCATAGCCGC TCAACTGAAT CAGTTAAATC TACCGTGTCT
    GAAACCTACC TCAGCAAGCC GAGCATCGCC AAGAGACGCG CCAACCAGCA GGAGACTGAG
    CAGTTCTATT TTATGAAGTT TCGCGAGTAT CTGGAGGGCA GCAATCTCAT CACTAAACTC
    CAGGCAAAGC ATGATCTACT GAAGAGAACC CTCGGTGACG GTTACCGGCC TGAATATGCA
    ACTACAAGGC CTCCCAGTCT TCCTCCTAAA CCGTTTGGCA CACAGAGGGC CAGGCCACGC
    TCTGTATTTA ACGTTAAGCT GTTTAATGGC AATTTGGAGT CTTTTGTTAA GGACTCTGGT
    CAAGCAATAC CTCGAGTTGT TGAGAGCTGT ATTCGATTCA TCAACCTGTA TGGCCTCCAA
    CATCAAGGAA TATTCAGAGT GCCTGGATCT CAAGTTGAAG TCAATGATAT CAAGAATTCA
    TTTGAGAGAG GGAACGATCC ACTGACAGAC GAAGAGAATA ACCACGATAT AAACTCTGTC
    GCTGGCGTTT TGAAACTCTA CTTTCGGGGC TTGGAGAACC CTCTGTTTCC AAAGGAAAGA
    TTCAATGATC TCATCTCCTG TATCAGAATA GAAAATCTGT ATGAAAGAGC GTTGTATATT
    CGAAAGATCC TTCTGACTGC ACCCAGATCA GTGCTTGTGG TCATGCGATA TCTGTTCGCC
    TTCCTCAACC ACTTGTCCCA GTACAGTGAT GAAAACATGA TGGACCCCTA TAATCTGGCC
    ATCTGCTTTG GGCCGACCCT GATGCCCACT CCCGAAACCC AGGACCAGGT GTCCTGCCAG
    GCCCACGTCA ATGAGGTCAT CAAGACCATC ATCATCCACC ACGAAACCAT CTTTCCTGAC
    TCCAAGGAGC TAGACGGCCC CCTGTATGAG AAGTGTATGG CAGGTGGAGA TTATTGTGAG
    AGTCCATACA GTGAACATGG AGCTCTTGAA GAGGTTGATC AGGATGGAGG AGCAGAGACC
    CACACCAGTG AGGATGAGGG AGAGCCCATT GAAGCCATCG CCAAGTTCGA TTACGTGGGC
    CGCTCTGCGC GGGAGCTGTC ATTTAAGAAG GGGGCATCTC TGCTGCTGTA CCAGAGAGCA
    TCCGACGATT GGTGGGAGGG CAGACACAAT GGCATAGATG GACTCGTGCC TCACCAGTAC
    ATTGTTGTTC AGGATATGGA CGACACGTTC TCAGACACTT TGAGTCAGAA AGCGGACAGT
    GAGGCCAGCA GTGGACATGG AGGAGAGGAG AAGTGCTCCA GCAGAGACAT GGGCTCTCCG
    ACAGACAGCA GACTGCCTGA TGCCTACATT AGCAGGCACA GAAAGAGAAG TGACCCTCCA
    ACCCGCAGAC CTCCGGCTCG CCCAACTGAC ACCCACTGCC TCGTCCACCC ATCTCATCAC
    AGCAGCCATA CCAACCCAGA TCTGGGCTCT CCGGTGATGG GCCACTACAG CCCGCGGGAC
    ATGCTGAGGG GCCGAGGACA CATGCCCATG GACAGTCCAG AGAGACGACG GCGAACAGGC
    CATGGCAGCC TGACCAACAT CAGCCGCCAC GAGTCCATTA AGAAGATGGA GAGTCCACCC
    ATCCGCCGGT CCACCTCTTC AGGACAGTAC AGCAGCTTCA ACGAGCCTCA TGTCAAGAGT
    CTGGACCCCG AGAGCATCGC ACAGGTCAGC GGGAGGCGAG CGACGACGCC ACTCACACCA
    TGCGCTGCAG GCCCTGCCCT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55887
    Clone ID Related Accession (Same CDS sequence) XM_017352030.1 , XM_017352030.2
    Accession Version XM_017352030.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3291bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product SLIT-ROBO Rho GTPase-activating protein 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTGAAAA TGTCGAATCC TCCTGTGAAG TTCAAAAAGG ATAAAGAGAT CATTGTAGAG 
    TACGAGACTC AAGTCAAAGA GATTCGAGCT CAACTGGTGG AGCAGCAGAA ATGTCTAGAG
    CAGCAGACCG ACATGAGGGT CCAGCTCCTT CAGGACCTCC AGGATTTTTT CCGCAAGAAG
    TCTGAGATCG AGATGGAGTA TTCACGAAAC CTGGAGAAAC TCGCCGAACG CTTCATGGCC
    AAGACAAGGA GCACCAAGGA CCACCAGCAA TATAAGAAAG ACCAGAATCT GCTGTCCCCG
    GTGAACTGCT GGTACCTGTT GCTGAACCAG GTCCGACGAG AGAGCAAAGA CCACGCAACC
    CTGAGTGACA TTTATCTGAA TAATGTCATC ATGAGATTCA TGCAGATCAG TGAAGACTCC
    ACACGTCTTA TGAAAAAGAG TAAAGAAATC ACCTTTCAGC TGCAAGAAGA TTTGATGAAA
    GTCCTCAACG AGCTCTACAC GGTGATGAAG ACCTATCATA TGTATCACAC AGAGAGCATC
    AGTGCTGAGG GTAAACTGAA GGAGGCTGAG AAGCAGGAGG AGAAGCAGAT CGGCCGCGTT
    GGAGATCCAG TTTTCCACAT CCGGCTGGAG GACAGACACC AGAGACGCAG CTCCGTCAAG
    AAGATCGAGA AGATGAAGGA GAAGAGGCAG GCGAAGTATT CGGAGAACAA GCTGAAGTCG
    ATCAAAGCTC GGAACGAATA CCTGCTGACG CTGGAAGCCA CTAATGCTTC AATCTTCAAG
    TATTACATCC ACGACTTGTC TGATTTAATA GACTGTTGTG ATCTGGGATA TCATGCCTCT
    CTGAACCGGG CATTGCGGAC CTACCTCTCA GCCGAATATA ACCTGGAGAC GTCCCGTCAC
    GAGGGCCTGG ACATCATAGA GAATGCAGTG GACAGTCTGG ACCCCCGTAG TGACCGCCAG
    CGCTTCATGG AAATGTACCC GACCGCCTTC TGCCCTCCCG TCAAATTTGA GTTTCAGGCA
    CATATGGGAG ACGAGGTTTG TCAGATCACG GCACAGCCTC CTGTTCAGGC TGAGCTCCTC
    CTGAGGTTCC AGCAGCTTCA ATCCCGCCTC ACCACACTGA AGATCGAGAA CGAAGAGATC
    AAGAAGACGT CTGAGGCGAC ACTTACTACC ATCCAGGACA TGGTGACGGT TGAGGATTAT
    GACGTATCTG AATGTTTCCA TCATAGCCGC TCAACTGAAT CAGTTAAATC TACCGTGTCT
    GAAACCTACC TCAGCAAGCC GAGCATCGCC AAGAGACGCG CCAACCAGCA GGAGACTGAG
    CAGTTCTATT TTATGAAGTT TCGCGAGTAT CTGGAGGGCA GCAATCTCAT CACTAAACTC
    CAGGCAAAGC ATGATCTACT GAAGAGAACC CTCGGTGACG GTTACCGGCC TGAATATGCA
    ACTACAAGGC CTCCCAGTCT TCCTCCTAAA CCGTTTGGCA CACAGAGGGC CAGGCCACGC
    TCTGTATTTA ACGTTAAGCT GTTTAATGGC AATTTGGAGT CTTTTGTTAA GGACTCTGGT
    CAAGCAATAC CTCGAGTTGT TGAGAGCTGT ATTCGATTCA TCAACCTGTA TGGCCTCCAA
    CATCAAGGAA TATTCAGAGT GCCTGGATCT CAAGTTGAAG TCAATGATAT CAAGAATTCA
    TTTGAGAGAG GGAACGATCC ACTGACAGAC GAAGAGAATA ACCACGATAT AAACTCTGTC
    GCTGGCGTTT TGAAACTCTA CTTTCGGGGC TTGGAGAACC CTCTGTTTCC AAAGGAAAGA
    TTCAATGATC TCATCTCCTG TATCAGAATA GAAAATCTGT ATGAAAGAGC GTTGTATATT
    CGAAAGATCC TTCTGACTGC ACCCAGATCA GTGCTTGTGG TCATGCGATA TCTGTTCGCC
    TTCCTCAACC ACTTGTCCCA GTACAGTGAT GAAAACATGA TGGACCCCTA TAATCTGGCC
    ATCTGCTTTG GGCCGACCCT GATGCCCACT CCCGAAACCC AGGACCAGGT GTCCTGCCAG
    GCCCACGTCA ATGAGGTCAT CAAGACCATC ATCATCCACC ACGAAACCAT CTTTCCTGAC
    TCCAAGGAGC TAGACGGCCC CCTGTATGAG AAGTGTATGG CAGGTGGAGA TTATTGTGAG
    AGTCCATACA GTGAACATGG AGCTCTTGAA GAGGTTGATC AGGATGGAGG AGCAGAGACC
    CACACCAGTG AGGATGAGGG AGAGCCCATT GAAGCCATCG CCAAGTTCGA TTACGTGGGC
    CGCTCTGCGC GGGAGCTGTC ATTTAAGAAG GGGGCATCTC TGCTGCTGTA CCAGAGAGCA
    TCCGACGATT GGTGGGAGGG CAGACACAAT GGCATAGATG GACTCGTGCC TCACCAGTAC
    ATTGTTGTTC AGGATATGGA CGACACGTTC TCAGACACTT TGAGTCAGAA AGCGGACAGT
    GAGGCCAGCA GTGGACATGG AGGAGAGGAG AAGTGCTCCA GCAGAGACAT GGGCTCTCCG
    ACAGACAGCA GACTGCCTGA TGCCTACATT AGCAGGCACA GAAAGAGAAG TGACCCTCCA
    ACCCGCAGAC CTCCGGCTCG CCCAACTGAC ACCCACTGCC TCGTCCACCC ATCTCATCAC
    AGCAGCCATA CCAACCCAGA TCTGGGCTCT CCGGTGATGG GCCACTACAG CCCGCGGGAC
    ATGCTGAGGG GCCGAGGACA CATGCCCATG GACAGTCCAG AGAGACGACG GCGAACAGGC
    CATGGCAGCC TGACCAACAT CAGCCGCCAC GAGTCCATTA AGAAGATGGA GAGTCCACCC
    ATCCGCCGGT CCACCTCTTC AGGACAGTAC AGCAGCTTCA ACGAGCCTCA TGTCAAGAGT
    CTGGACCCCG AGAGCATCGC ACAGGACATA GAGGAGACGA TGAACACTGC TCTGAATGAG
    CTGAAGGAAC TGGAGCGCCA GAGCTCTGCC AAACACGCCC CAGACGTGGT GCTGGACACC
    CTGGAGCAGA TGAAGAACGC TCCCACCCCG GCCAGCTCCA CTGAGTCCCT CAGCCAACTC
    CACGGCCTCC TGCTGCGGCC TGCTGGCACT GAGCTACACA TGCGCCGCAG CACCAGCTCC
    TCCAGCGACA CCATGAGCAC CTTCAAGCCT GCGGTGGCGC CTCGTATGGG GGTACAGCTC
    AAACCGCCCA CCCTGAGGCC CAAACCCATG GTGCCGGTGC CCAAAACTGG AGCGGCGCAG
    CATCCGGCAG CTCCGCCACA GGATCCATTG GACAAATCCT GCACCATGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207519.1
    CDS877..4167
    Translation

    Target ORF information:

    RefSeq Version XM_017352030.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio SLIT-ROBO Rho GTPase activating protein 1a (srgap1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352030.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTGAAAA TGTCGAATCC TCCTGTGAAG TTCAAAAAGG ATAAAGAGAT CATTGTAGAG 
    TACGAGACTC AAGTCAAAGA GATTCGAGCT CAACTGGTGG AGCAGCAGAA ATGTCTAGAG
    CAGCAGACCG ACATGAGGGT CCAGCTCCTT CAGGACCTCC AGGATTTTTT CCGCAAGAAG
    TCTGAGATCG AGATGGAGTA TTCACGAAAC CTGGAGAAAC TCGCCGAACG CTTCATGGCC
    AAGACAAGGA GCACCAAGGA CCACCAGCAA TATAAGAAAG ACCAGAATCT GCTGTCCCCG
    GTGAACTGCT GGTACCTGTT GCTGAACCAG GTCCGACGAG AGAGCAAAGA CCACGCAACC
    CTGAGTGACA TTTATCTGAA TAATGTCATC ATGAGATTCA TGCAGATCAG TGAAGACTCC
    ACACGTCTTA TGAAAAAGAG TAAAGAAATC ACCTTTCAGC TGCAAGAAGA TTTGATGAAA
    GTCCTCAACG AGCTCTACAC GGTGATGAAG ACCTATCATA TGTATCACAC AGAGAGCATC
    AGTGCTGAGG GTAAACTGAA GGAGGCTGAG AAGCAGGAGG AGAAGCAGAT CGGCCGCGTT
    GGAGATCCAG TTTTCCACAT CCGGCTGGAG GACAGACACC AGAGACGCAG CTCCGTCAAG
    AAGATCGAGA AGATGAAGGA GAAGAGGCAG GCGAAGTATT CGGAGAACAA GCTGAAGTCG
    ATCAAAGCTC GGAACGAATA CCTGCTGACG CTGGAAGCCA CTAATGCTTC AATCTTCAAG
    TATTACATCC ACGACTTGTC TGATTTAATA GACTGTTGTG ATCTGGGATA TCATGCCTCT
    CTGAACCGGG CATTGCGGAC CTACCTCTCA GCCGAATATA ACCTGGAGAC GTCCCGTCAC
    GAGGGCCTGG ACATCATAGA GAATGCAGTG GACAGTCTGG ACCCCCGTAG TGACCGCCAG
    CGCTTCATGG AAATGTACCC GACCGCCTTC TGCCCTCCCG TCAAATTTGA GTTTCAGGCA
    CATATGGGAG ACGAGGTTTG TCAGATCACG GCACAGCCTC CTGTTCAGGC TGAGCTCCTC
    CTGAGGTTCC AGCAGCTTCA ATCCCGCCTC ACCACACTGA AGATCGAGAA CGAAGAGATC
    AAGAAGACGT CTGAGGCGAC ACTTACTACC ATCCAGGACA TGGTGACGGT TGAGGATTAT
    GACGTATCTG AATGTTTCCA TCATAGCCGC TCAACTGAAT CAGTTAAATC TACCGTGTCT
    GAAACCTACC TCAGCAAGCC GAGCATCGCC AAGAGACGCG CCAACCAGCA GGAGACTGAG
    CAGTTCTATT TTATGAAGTT TCGCGAGTAT CTGGAGGGCA GCAATCTCAT CACTAAACTC
    CAGGCAAAGC ATGATCTACT GAAGAGAACC CTCGGTGACG GTTACCGGCC TGAATATGCA
    ACTACAAGGC CTCCCAGTCT TCCTCCTAAA CCGTTTGGCA CACAGAGGGC CAGGCCACGC
    TCTGTATTTA ACGTTAAGCT GTTTAATGGC AATTTGGAGT CTTTTGTTAA GGACTCTGGT
    CAAGCAATAC CTCGAGTTGT TGAGAGCTGT ATTCGATTCA TCAACCTGTA TGGCCTCCAA
    CATCAAGGAA TATTCAGAGT GCCTGGATCT CAAGTTGAAG TCAATGATAT CAAGAATTCA
    TTTGAGAGAG GGAACGATCC ACTGACAGAC GAAGAGAATA ACCACGATAT AAACTCTGTC
    GCTGGCGTTT TGAAACTCTA CTTTCGGGGC TTGGAGAACC CTCTGTTTCC AAAGGAAAGA
    TTCAATGATC TCATCTCCTG TATCAGAATA GAAAATCTGT ATGAAAGAGC GTTGTATATT
    CGAAAGATCC TTCTGACTGC ACCCAGATCA GTGCTTGTGG TCATGCGATA TCTGTTCGCC
    TTCCTCAACC ACTTGTCCCA GTACAGTGAT GAAAACATGA TGGACCCCTA TAATCTGGCC
    ATCTGCTTTG GGCCGACCCT GATGCCCACT CCCGAAACCC AGGACCAGGT GTCCTGCCAG
    GCCCACGTCA ATGAGGTCAT CAAGACCATC ATCATCCACC ACGAAACCAT CTTTCCTGAC
    TCCAAGGAGC TAGACGGCCC CCTGTATGAG AAGTGTATGG CAGGTGGAGA TTATTGTGAG
    AGTCCATACA GTGAACATGG AGCTCTTGAA GAGGTTGATC AGGATGGAGG AGCAGAGACC
    CACACCAGTG AGGATGAGGG AGAGCCCATT GAAGCCATCG CCAAGTTCGA TTACGTGGGC
    CGCTCTGCGC GGGAGCTGTC ATTTAAGAAG GGGGCATCTC TGCTGCTGTA CCAGAGAGCA
    TCCGACGATT GGTGGGAGGG CAGACACAAT GGCATAGATG GACTCGTGCC TCACCAGTAC
    ATTGTTGTTC AGGATATGGA CGACACGTTC TCAGACACTT TGAGTCAGAA AGCGGACAGT
    GAGGCCAGCA GTGGACATGG AGGAGAGGAG AAGTGCTCCA GCAGAGACAT GGGCTCTCCG
    ACAGACAGCA GACTGCCTGA TGCCTACATT AGCAGGCACA GAAAGAGAAG TGACCCTCCA
    ACCCGCAGAC CTCCGGCTCG CCCAACTGAC ACCCACTGCC TCGTCCACCC ATCTCATCAC
    AGCAGCCATA CCAACCCAGA TCTGGGCTCT CCGGTGATGG GCCACTACAG CCCGCGGGAC
    ATGCTGAGGG GCCGAGGACA CATGCCCATG GACAGTCCAG AGAGACGACG GCGAACAGGC
    CATGGCAGCC TGACCAACAT CAGCCGCCAC GAGTCCATTA AGAAGATGGA GAGTCCACCC
    ATCCGCCGGT CCACCTCTTC AGGACAGTAC AGCAGCTTCA ACGAGCCTCA TGTCAAGAGT
    CTGGACCCCG AGAGCATCGC ACAGGACATA GAGGAGACGA TGAACACTGC TCTGAATGAG
    CTGAAGGAAC TGGAGCGCCA GAGCTCTGCC AAACACGCCC CAGACGTGGT GCTGGACACC
    CTGGAGCAGA TGAAGAACGC TCCCACCCCG GCCAGCTCCA CTGAGTCCCT CAGCCAACTC
    CACGGCCTCC TGCTGCGGCC TGCTGGCACT GAGCTACACA TGCGCCGCAG CACCAGCTCC
    TCCAGCGACA CCATGAGCAC CTTCAAGCCT GCGGTGGCGC CTCGTATGGG GGTACAGCTC
    AAACCGCCCA CCCTGAGGCC CAAACCCATG GTGCCGGTGC CCAAAACTGG AGCGGCGCAG
    CATCCGGCAG CTCCGCCACA GGATCCATTG GACAAATCCT GCACCATGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55887
    Clone ID Related Accession (Same CDS sequence) XM_017352030.1 , XM_017352030.2
    Accession Version XM_017352030.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3291bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product SLIT-ROBO Rho GTPase-activating protein 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352030.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTGAAAA TGTCGAATCC TCCTGTGAAG TTCAAAAAGG ATAAAGAGAT CATTGTAGAG 
    TACGAGACTC AAGTCAAAGA GATTCGAGCT CAACTGGTGG AGCAGCAGAA ATGTCTAGAG
    CAGCAGACCG ACATGAGGGT CCAGCTCCTT CAGGACCTCC AGGATTTTTT CCGCAAGAAG
    TCTGAGATCG AGATGGAGTA TTCACGAAAC CTGGAGAAAC TCGCCGAACG CTTCATGGCC
    AAGACAAGGA GCACCAAGGA CCACCAGCAA TATAAGAAAG ACCAGAATCT GCTGTCCCCG
    GTGAACTGCT GGTACCTGTT GCTGAACCAG GTCCGACGAG AGAGCAAAGA CCACGCAACC
    CTGAGTGACA TTTATCTGAA TAATGTCATC ATGAGATTCA TGCAGATCAG TGAAGACTCC
    ACACGTCTTA TGAAAAAGAG TAAAGAAATC ACCTTTCAGC TGCAAGAAGA TTTGATGAAA
    GTCCTCAACG AGCTCTACAC GGTGATGAAG ACCTATCATA TGTATCACAC AGAGAGCATC
    AGTGCTGAGG GTAAACTGAA GGAGGCTGAG AAGCAGGAGG AGAAGCAGAT CGGCCGCGTT
    GGAGATCCAG TTTTCCACAT CCGGCTGGAG GACAGACACC AGAGACGCAG CTCCGTCAAG
    AAGATCGAGA AGATGAAGGA GAAGAGGCAG GCGAAGTATT CGGAGAACAA GCTGAAGTCG
    ATCAAAGCTC GGAACGAATA CCTGCTGACG CTGGAAGCCA CTAATGCTTC AATCTTCAAG
    TATTACATCC ACGACTTGTC TGATTTAATA GACTGTTGTG ATCTGGGATA TCATGCCTCT
    CTGAACCGGG CATTGCGGAC CTACCTCTCA GCCGAATATA ACCTGGAGAC GTCCCGTCAC
    GAGGGCCTGG ACATCATAGA GAATGCAGTG GACAGTCTGG ACCCCCGTAG TGACCGCCAG
    CGCTTCATGG AAATGTACCC GACCGCCTTC TGCCCTCCCG TCAAATTTGA GTTTCAGGCA
    CATATGGGAG ACGAGGTTTG TCAGATCACG GCACAGCCTC CTGTTCAGGC TGAGCTCCTC
    CTGAGGTTCC AGCAGCTTCA ATCCCGCCTC ACCACACTGA AGATCGAGAA CGAAGAGATC
    AAGAAGACGT CTGAGGCGAC ACTTACTACC ATCCAGGACA TGGTGACGGT TGAGGATTAT
    GACGTATCTG AATGTTTCCA TCATAGCCGC TCAACTGAAT CAGTTAAATC TACCGTGTCT
    GAAACCTACC TCAGCAAGCC GAGCATCGCC AAGAGACGCG CCAACCAGCA GGAGACTGAG
    CAGTTCTATT TTATGAAGTT TCGCGAGTAT CTGGAGGGCA GCAATCTCAT CACTAAACTC
    CAGGCAAAGC ATGATCTACT GAAGAGAACC CTCGGTGACG GTTACCGGCC TGAATATGCA
    ACTACAAGGC CTCCCAGTCT TCCTCCTAAA CCGTTTGGCA CACAGAGGGC CAGGCCACGC
    TCTGTATTTA ACGTTAAGCT GTTTAATGGC AATTTGGAGT CTTTTGTTAA GGACTCTGGT
    CAAGCAATAC CTCGAGTTGT TGAGAGCTGT ATTCGATTCA TCAACCTGTA TGGCCTCCAA
    CATCAAGGAA TATTCAGAGT GCCTGGATCT CAAGTTGAAG TCAATGATAT CAAGAATTCA
    TTTGAGAGAG GGAACGATCC ACTGACAGAC GAAGAGAATA ACCACGATAT AAACTCTGTC
    GCTGGCGTTT TGAAACTCTA CTTTCGGGGC TTGGAGAACC CTCTGTTTCC AAAGGAAAGA
    TTCAATGATC TCATCTCCTG TATCAGAATA GAAAATCTGT ATGAAAGAGC GTTGTATATT
    CGAAAGATCC TTCTGACTGC ACCCAGATCA GTGCTTGTGG TCATGCGATA TCTGTTCGCC
    TTCCTCAACC ACTTGTCCCA GTACAGTGAT GAAAACATGA TGGACCCCTA TAATCTGGCC
    ATCTGCTTTG GGCCGACCCT GATGCCCACT CCCGAAACCC AGGACCAGGT GTCCTGCCAG
    GCCCACGTCA ATGAGGTCAT CAAGACCATC ATCATCCACC ACGAAACCAT CTTTCCTGAC
    TCCAAGGAGC TAGACGGCCC CCTGTATGAG AAGTGTATGG CAGGTGGAGA TTATTGTGAG
    AGTCCATACA GTGAACATGG AGCTCTTGAA GAGGTTGATC AGGATGGAGG AGCAGAGACC
    CACACCAGTG AGGATGAGGG AGAGCCCATT GAAGCCATCG CCAAGTTCGA TTACGTGGGC
    CGCTCTGCGC GGGAGCTGTC ATTTAAGAAG GGGGCATCTC TGCTGCTGTA CCAGAGAGCA
    TCCGACGATT GGTGGGAGGG CAGACACAAT GGCATAGATG GACTCGTGCC TCACCAGTAC
    ATTGTTGTTC AGGATATGGA CGACACGTTC TCAGACACTT TGAGTCAGAA AGCGGACAGT
    GAGGCCAGCA GTGGACATGG AGGAGAGGAG AAGTGCTCCA GCAGAGACAT GGGCTCTCCG
    ACAGACAGCA GACTGCCTGA TGCCTACATT AGCAGGCACA GAAAGAGAAG TGACCCTCCA
    ACCCGCAGAC CTCCGGCTCG CCCAACTGAC ACCCACTGCC TCGTCCACCC ATCTCATCAC
    AGCAGCCATA CCAACCCAGA TCTGGGCTCT CCGGTGATGG GCCACTACAG CCCGCGGGAC
    ATGCTGAGGG GCCGAGGACA CATGCCCATG GACAGTCCAG AGAGACGACG GCGAACAGGC
    CATGGCAGCC TGACCAACAT CAGCCGCCAC GAGTCCATTA AGAAGATGGA GAGTCCACCC
    ATCCGCCGGT CCACCTCTTC AGGACAGTAC AGCAGCTTCA ACGAGCCTCA TGTCAAGAGT
    CTGGACCCCG AGAGCATCGC ACAGGACATA GAGGAGACGA TGAACACTGC TCTGAATGAG
    CTGAAGGAAC TGGAGCGCCA GAGCTCTGCC AAACACGCCC CAGACGTGGT GCTGGACACC
    CTGGAGCAGA TGAAGAACGC TCCCACCCCG GCCAGCTCCA CTGAGTCCCT CAGCCAACTC
    CACGGCCTCC TGCTGCGGCC TGCTGGCACT GAGCTACACA TGCGCCGCAG CACCAGCTCC
    TCCAGCGACA CCATGAGCAC CTTCAAGCCT GCGGTGGCGC CTCGTATGGG GGTACAGCTC
    AAACCGCCCA CCCTGAGGCC CAAACCCATG GTGCCGGTGC CCAAAACTGG AGCGGCGCAG
    CATCCGGCAG CTCCGCCACA GGATCCATTG GACAAATCCT GCACCATGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207519.1
    CDS875..4165
    Translation

    Target ORF information:

    RefSeq Version XM_017352030.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio SLIT-ROBO Rho GTPase activating protein 1a (srgap1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352030.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGTTGAAAA TGTCGAATCC TCCTGTGAAG TTCAAAAAGG ATAAAGAGAT CATTGTAGAG 
    TACGAGACTC AAGTCAAAGA GATTCGAGCT CAACTGGTGG AGCAGCAGAA ATGTCTAGAG
    CAGCAGACCG ACATGAGGGT CCAGCTCCTT CAGGACCTCC AGGATTTTTT CCGCAAGAAG
    TCTGAGATCG AGATGGAGTA TTCACGAAAC CTGGAGAAAC TCGCCGAACG CTTCATGGCC
    AAGACAAGGA GCACCAAGGA CCACCAGCAA TATAAGAAAG ACCAGAATCT GCTGTCCCCG
    GTGAACTGCT GGTACCTGTT GCTGAACCAG GTCCGACGAG AGAGCAAAGA CCACGCAACC
    CTGAGTGACA TTTATCTGAA TAATGTCATC ATGAGATTCA TGCAGATCAG TGAAGACTCC
    ACACGTCTTA TGAAAAAGAG TAAAGAAATC ACCTTTCAGC TGCAAGAAGA TTTGATGAAA
    GTCCTCAACG AGCTCTACAC GGTGATGAAG ACCTATCATA TGTATCACAC AGAGAGCATC
    AGTGCTGAGG GTAAACTGAA GGAGGCTGAG AAGCAGGAGG AGAAGCAGAT CGGCCGCGTT
    GGAGATCCAG TTTTCCACAT CCGGCTGGAG GACAGACACC AGAGACGCAG CTCCGTCAAG
    AAGATCGAGA AGATGAAGGA GAAGAGGCAG GCGAAGTATT CGGAGAACAA GCTGAAGTCG
    ATCAAAGCTC GGAACGAATA CCTGCTGACG CTGGAAGCCA CTAATGCTTC AATCTTCAAG
    TATTACATCC ACGACTTGTC TGATTTAATA GACTGTTGTG ATCTGGGATA TCATGCCTCT
    CTGAACCGGG CATTGCGGAC CTACCTCTCA GCCGAATATA ACCTGGAGAC GTCCCGTCAC
    GAGGGCCTGG ACATCATAGA GAATGCAGTG GACAGTCTGG ACCCCCGTAG TGACCGCCAG
    CGCTTCATGG AAATGTACCC GACCGCCTTC TGCCCTCCCG TCAAATTTGA GTTTCAGGCA
    CATATGGGAG ACGAGGTTTG TCAGATCACG GCACAGCCTC CTGTTCAGGC TGAGCTCCTC
    CTGAGGTTCC AGCAGCTTCA ATCCCGCCTC ACCACACTGA AGATCGAGAA CGAAGAGATC
    AAGAAGACGT CTGAGGCGAC ACTTACTACC ATCCAGGACA TGGTGACGGT TGAGGATTAT
    GACGTATCTG AATGTTTCCA TCATAGCCGC TCAACTGAAT CAGTTAAATC TACCGTGTCT
    GAAACCTACC TCAGCAAGCC GAGCATCGCC AAGAGACGCG CCAACCAGCA GGAGACTGAG
    CAGTTCTATT TTATGAAGTT TCGCGAGTAT CTGGAGGGCA GCAATCTCAT CACTAAACTC
    CAGGCAAAGC ATGATCTACT GAAGAGAACC CTCGGTGACG GTTACCGGCC TGAATATGCA
    ACTACAAGGC CTCCCAGTCT TCCTCCTAAA CCGTTTGGCA CACAGAGGGC CAGGCCACGC
    TCTGTATTTA ACGTTAAGCT GTTTAATGGC AATTTGGAGT CTTTTGTTAA GGACTCTGGT
    CAAGCAATAC CTCGAGTTGT TGAGAGCTGT ATTCGATTCA TCAACCTGTA TGGCCTCCAA
    CATCAAGGAA TATTCAGAGT GCCTGGATCT CAAGTTGAAG TCAATGATAT CAAGAATTCA
    TTTGAGAGAG GGAACGATCC ACTGACAGAC GAAGAGAATA ACCACGATAT AAACTCTGTC
    GCTGGCGTTT TGAAACTCTA CTTTCGGGGC TTGGAGAACC CTCTGTTTCC AAAGGAAAGA
    TTCAATGATC TCATCTCCTG TATCAGAATA GAAAATCTGT ATGAAAGAGC GTTGTATATT
    CGAAAGATCC TTCTGACTGC ACCCAGATCA GTGCTTGTGG TCATGCGATA TCTGTTCGCC
    TTCCTCAACC ACTTGTCCCA GTACAGTGAT GAAAACATGA TGGACCCCTA TAATCTGGCC
    ATCTGCTTTG GGCCGACCCT GATGCCCACT CCCGAAACCC AGGACCAGGT GTCCTGCCAG
    GCCCACGTCA ATGAGGTCAT CAAGACCATC ATCATCCACC ACGAAACCAT CTTTCCTGAC
    TCCAAGGAGC TAGACGGCCC CCTGTATGAG AAGTGTATGG CAGGTGGAGA TTATTGTGAG
    AGTCCATACA GTGAACATGG AGCTCTTGAA GAGGTTGATC AGGATGGAGG AGCAGAGACC
    CACACCAGTG AGGATGAGGG AGAGCCCATT GAAGCCATCG CCAAGTTCGA TTACGTGGGC
    CGCTCTGCGC GGGAGCTGTC ATTTAAGAAG GGGGCATCTC TGCTGCTGTA CCAGAGAGCA
    TCCGACGATT GGTGGGAGGG CAGACACAAT GGCATAGATG GACTCGTGCC TCACCAGTAC
    ATTGTTGTTC AGGATATGGA CGACACGTTC TCAGACACTT TGAGTCAGAA AGCGGACAGT
    GAGGCCAGCA GTGGACATGG AGGAGAGGAG AAGTGCTCCA GCAGAGACAT GGGCTCTCCG
    ACAGACAGCA GACTGCCTGA TGCCTACATT AGCAGGCACA GAAAGAGAAG TGACCCTCCA
    ACCCGCAGAC CTCCGGCTCG CCCAACTGAC ACCCACTGCC TCGTCCACCC ATCTCATCAC
    AGCAGCCATA CCAACCCAGA TCTGGGCTCT CCGGTGATGG GCCACTACAG CCCGCGGGAC
    ATGCTGAGGG GCCGAGGACA CATGCCCATG GACAGTCCAG AGAGACGACG GCGAACAGGC
    CATGGCAGCC TGACCAACAT CAGCCGCCAC GAGTCCATTA AGAAGATGGA GAGTCCACCC
    ATCCGCCGGT CCACCTCTTC AGGACAGTAC AGCAGCTTCA ACGAGCCTCA TGTCAAGAGT
    CTGGACCCCG AGAGCATCGC ACAGGACATA GAGGAGACGA TGAACACTGC TCTGAATGAG
    CTGAAGGAAC TGGAGCGCCA GAGCTCTGCC AAACACGCCC CAGACGTGGT GCTGGACACC
    CTGGAGCAGA TGAAGAACGC TCCCACCCCG GCCAGCTCCA CTGAGTCCCT CAGCCAACTC
    CACGGCCTCC TGCTGCGGCC TGCTGGCACT GAGCTACACA TGCGCCGCAG CACCAGCTCC
    TCCAGCGACA CCATGAGCAC CTTCAAGCCT GCGGTGGCGC CTCGTATGGG GGTACAGCTC
    AAACCGCCCA CCCTGAGGCC CAAACCCATG GTGCCGGTGC CCAAAACTGG AGCGGCGCAG
    CATCCGGCAG CTCCGCCACA GGATCCATTG GACAAATCCT GCACCATGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55888
    Clone ID Related Accession (Same CDS sequence) XM_017352031.1 , XM_017352031.2
    Accession Version XM_017352031.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product SLIT-ROBO Rho GTPase-activating protein 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTGAAAA TGTCGAATCC TCCTGTGAAG TTCAAAAAGG ATAAAGAGAT CATTGTAGAG 
    TACGAGACTC AAGTCAAAGA GATTCGAGCT CAACTGGTGG AGCAGCAGAA ATGTCTAGAG
    CAGCAGACCG ACATGAGGGT CCAGCTCCTT CAGGACCTCC AGGATTTTTT CCGCAAGAAG
    TCTGAGATCG AGATGGAGTA TTCACGAAAC CTGGAGAAAC TCGCCGAACG CTTCATGGCC
    AAGACAAGGA GCACCAAGGA CCACCAGCAA TATAAGAAAG ACCAGAATCT GCTGTCCCCG
    GTGAACTGCT GGTACCTGTT GCTGAACCAG GTCCGACGAG AGAGCAAAGA CCACGCAACC
    CTGAGTGACA TTTATCTGAA TAATGTCATC ATGAGATTCA TGCAGATCAG TGAAGACTCC
    ACACGTCTTA TGAAAAAGAG TAAAGAAATC ACCTTTCAGC TGCAAGAAGA TTTGATGAAA
    GTCCTCAACG AGCTCTACAC GGTGATGAAG ACCTATCATA TGTATCACAC AGAGAGCATC
    AGTGCTGAGG GTAAACTGAA GGAGGCTGAG AAGCAGGAGG AGAAGCAGAT CGGCCGCGTT
    GGAGATCCAG TTTTCCACAT CCGGCTGGAG GACAGACACC AGAGACGCAG CTCCGTCAAG
    AAGATCGAGA AGATGAAGGA GAAGAGGCAG GCGAAGTATT CGGAGAACAA GCTGAAGTCG
    ATCAAAGCTC GGAACGAATA CCTGCTGACG CTGGAAGCCA CTAATGCTTC AATCTTCAAG
    TATTACATCC ACGACTTGTC TGATTTAATA GACTGTTGTG ATCTGGGATA TCATGCCTCT
    CTGAACCGGG CATTGCGGAC CTACCTCTCA GCCGAATATA ACCTGGAGAC GTCCCGTCAC
    GAGGGCCTGG ACATCATAGA GAATGCAGTG GACAGTCTGG ACCCCCGTAG TGACCGCCAG
    CGCTTCATGG AAATGTACCC GACCGCCTTC TGCCCTCCCG TCAAATTTGA GTTTCAGGCA
    CATATGGGAG ACGAGGTTTG TCAGATCACG GCACAGCCTC CTGTTCAGGC TGAGCTCCTC
    CTGAGGTTCC AGCAGCTTCA ATCCCGCCTC ACCACACTGA AGATCGAGAA CGAAGAGATC
    AAGAAGACGT CTGAGGCGAC ACTTACTACC ATCCAGGACA TGGTGACGGT TGAGGATTAT
    GACGTATCTG AATGTTTCCA TCATAGCCGC TCAACTGAAT CAGTTAAATC TACCGTGTCT
    GAAACCTACC TCAGCAAGCC GAGCATCGCC AAGAGACGCG CCAACCAGCA GGAGACTGAG
    CAGTTCTATT TTATGAAGTT TCGCGAGTAT CTGGAGGGCA GCAATCTCAT CACTAAACTC
    CAGGCAAAGC ATGATCTACT GAAGAGAACC CTCGGTGACG GTTACCGGCC TGAATATGCA
    ACTACAAGTC GTGGACGAAG GAACTCGCAC GCAAGACATC AGGACTCTGG TCAAGCAATA
    CCTCGAGTTG TTGAGAGCTG TATTCGATTC ATCAACCTGT ATGGCCTCCA ACATCAAGGA
    ATATTCAGAG TGCCTGGATC TCAAGTTGAA GTCAATGATA TCAAGAATTC ATTTGAGAGA
    GGGAACGATC CACTGACAGA CGAAGAGAAT AACCACGATA TAAACTCTGT CGCTGGCGTT
    TTGAAACTCT ACTTTCGGGG CTTGGAGAAC CCTCTGTTTC CAAAGGAAAG ATTCAATGAT
    CTCATCTCCT GTATCAGAAT AGAAAATCTG TATGAAAGAG CGTTGTATAT TCGAAAGATC
    CTTCTGACTG CACCCAGATC AGTGCTTGTG GTCATGCGAT ATCTGTTCGC CTTCCTCAAC
    CACTTGTCCC AGTACAGTGA TGAAAACATG ATGGACCCCT ATAATCTGGC CATCTGCTTT
    GGGCCGACCC TGATGCCCAC TCCCGAAACC CAGGACCAGG TGTCCTGCCA GGCCCACGTC
    AATGAGGTCA TCAAGACCAT CATCATCCAC CACGAAACCA TCTTTCCTGA CTCCAAGGAG
    CTAGACGGCC CCCTGTATGA GAAGTGTATG GCAGGTGGAG ATTATTGTGA GAGTCCATAC
    AGTGAACATG GAGCTCTTGA AGAGGTTGAT CAGGATGGAG GAGCAGAGAC CCACACCAGT
    GAGGATGAGG GAGAGCCCAT TGAAGCCATC GCCAAGTTCG ATTACGTGGG CCGCTCTGCG
    CGGGAGCTGT CATTTAAGAA GGGGGCATCT CTGCTGCTGT ACCAGAGAGC ATCCGACGAT
    TGGTGGGAGG GCAGACACAA TGGCATAGAT GGACTCGTGC CTCACCAGTA CATTGTTGTT
    CAGGATATGG ACGACACGTT CTCAGACACT TTGAGTCAGA AAGCGGACAG TGAGGCCAGC
    AGTGGACATG GAGGAGAGGA GAAGTGCTCC AGCAGAGACA TGGGCTCTCC GACAGACAGC
    AGACTGCCTG ATGCCTACAT TAGCAGGCAC AGAAAGAGAA GTGACCCTCC AACCCGCAGA
    CCTCCGGCTC GCCCAACTGA CACCCACTGC CTCGTCCACC CATCTCATCA CAGCAGCCAT
    ACCAACCCAG ATCTGGGCTC TCCGGTGATG GGCCACTACA GCCCGCGGGA CATGCTGAGG
    GGCCGAGGAC ACATGCCCAT GGACAGTCCA GAGAGACGAC GGCGAACAGG CCATGGCAGC
    CTGACCAACA TCAGCCGCCA CGAGTCCATT AAGAAGATGG AGAGTCCACC CATCCGCCGG
    TCCACCTCTT CAGGACAGTA CAGCAGCTTC AACGAGCCTC ATGTCAAGAG TCTGGACCCC
    GAGAGCATCG CACAGGACAT AGAGGAGACG ATGAACACTG CTCTGAATGA GCTGAAGGAA
    CTGGAGCGCC AGAGCTCTGC CAAACACGCC CCAGACGTGG TGCTGGACAC CCTGGAGCAG
    ATGAAGAACG CTCCCACCCC GGCCAGCTCC ACTGAGTCCC TCAGCCAACT CCACGGCCTC
    CTGCTGCGGC CTGCTGGCAC TGAGCTACAC ATGCGCCGCA GCACCAGCTC CTCCAGCGAC
    ACCATGAGCA CCTTCAAGCC TGCGGTGGCG CCTCGTATGG GGGTACAGCT CAAACCGCCC
    ACCCTGAGGC CCAAACCCAT GGTGCCGGTG CCCAAAACTG GAGCGGCGCA GCATCCGGCA
    GCTCCGCCAC AGGATCCATT GGACAAATCC TGCACCATGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207520.1
    CDS877..4098
    Translation

    Target ORF information:

    RefSeq Version XM_017352031.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio SLIT-ROBO Rho GTPase activating protein 1a (srgap1a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352031.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTGAAAA TGTCGAATCC TCCTGTGAAG TTCAAAAAGG ATAAAGAGAT CATTGTAGAG 
    TACGAGACTC AAGTCAAAGA GATTCGAGCT CAACTGGTGG AGCAGCAGAA ATGTCTAGAG
    CAGCAGACCG ACATGAGGGT CCAGCTCCTT CAGGACCTCC AGGATTTTTT CCGCAAGAAG
    TCTGAGATCG AGATGGAGTA TTCACGAAAC CTGGAGAAAC TCGCCGAACG CTTCATGGCC
    AAGACAAGGA GCACCAAGGA CCACCAGCAA TATAAGAAAG ACCAGAATCT GCTGTCCCCG
    GTGAACTGCT GGTACCTGTT GCTGAACCAG GTCCGACGAG AGAGCAAAGA CCACGCAACC
    CTGAGTGACA TTTATCTGAA TAATGTCATC ATGAGATTCA TGCAGATCAG TGAAGACTCC
    ACACGTCTTA TGAAAAAGAG TAAAGAAATC ACCTTTCAGC TGCAAGAAGA TTTGATGAAA
    GTCCTCAACG AGCTCTACAC GGTGATGAAG ACCTATCATA TGTATCACAC AGAGAGCATC
    AGTGCTGAGG GTAAACTGAA GGAGGCTGAG AAGCAGGAGG AGAAGCAGAT CGGCCGCGTT
    GGAGATCCAG TTTTCCACAT CCGGCTGGAG GACAGACACC AGAGACGCAG CTCCGTCAAG
    AAGATCGAGA AGATGAAGGA GAAGAGGCAG GCGAAGTATT CGGAGAACAA GCTGAAGTCG
    ATCAAAGCTC GGAACGAATA CCTGCTGACG CTGGAAGCCA CTAATGCTTC AATCTTCAAG
    TATTACATCC ACGACTTGTC TGATTTAATA GACTGTTGTG ATCTGGGATA TCATGCCTCT
    CTGAACCGGG CATTGCGGAC CTACCTCTCA GCCGAATATA ACCTGGAGAC GTCCCGTCAC
    GAGGGCCTGG ACATCATAGA GAATGCAGTG GACAGTCTGG ACCCCCGTAG TGACCGCCAG
    CGCTTCATGG AAATGTACCC GACCGCCTTC TGCCCTCCCG TCAAATTTGA GTTTCAGGCA
    CATATGGGAG ACGAGGTTTG TCAGATCACG GCACAGCCTC CTGTTCAGGC TGAGCTCCTC
    CTGAGGTTCC AGCAGCTTCA ATCCCGCCTC ACCACACTGA AGATCGAGAA CGAAGAGATC
    AAGAAGACGT CTGAGGCGAC ACTTACTACC ATCCAGGACA TGGTGACGGT TGAGGATTAT
    GACGTATCTG AATGTTTCCA TCATAGCCGC TCAACTGAAT CAGTTAAATC TACCGTGTCT
    GAAACCTACC TCAGCAAGCC GAGCATCGCC AAGAGACGCG CCAACCAGCA GGAGACTGAG
    CAGTTCTATT TTATGAAGTT TCGCGAGTAT CTGGAGGGCA GCAATCTCAT CACTAAACTC
    CAGGCAAAGC ATGATCTACT GAAGAGAACC CTCGGTGACG GTTACCGGCC TGAATATGCA
    ACTACAAGTC GTGGACGAAG GAACTCGCAC GCAAGACATC AGGACTCTGG TCAAGCAATA
    CCTCGAGTTG TTGAGAGCTG TATTCGATTC ATCAACCTGT ATGGCCTCCA ACATCAAGGA
    ATATTCAGAG TGCCTGGATC TCAAGTTGAA GTCAATGATA TCAAGAATTC ATTTGAGAGA
    GGGAACGATC CACTGACAGA CGAAGAGAAT AACCACGATA TAAACTCTGT CGCTGGCGTT
    TTGAAACTCT ACTTTCGGGG CTTGGAGAAC CCTCTGTTTC CAAAGGAAAG ATTCAATGAT
    CTCATCTCCT GTATCAGAAT AGAAAATCTG TATGAAAGAG CGTTGTATAT TCGAAAGATC
    CTTCTGACTG CACCCAGATC AGTGCTTGTG GTCATGCGAT ATCTGTTCGC CTTCCTCAAC
    CACTTGTCCC AGTACAGTGA TGAAAACATG ATGGACCCCT ATAATCTGGC CATCTGCTTT
    GGGCCGACCC TGATGCCCAC TCCCGAAACC CAGGACCAGG TGTCCTGCCA GGCCCACGTC
    AATGAGGTCA TCAAGACCAT CATCATCCAC CACGAAACCA TCTTTCCTGA CTCCAAGGAG
    CTAGACGGCC CCCTGTATGA GAAGTGTATG GCAGGTGGAG ATTATTGTGA GAGTCCATAC
    AGTGAACATG GAGCTCTTGA AGAGGTTGAT CAGGATGGAG GAGCAGAGAC CCACACCAGT
    GAGGATGAGG GAGAGCCCAT TGAAGCCATC GCCAAGTTCG ATTACGTGGG CCGCTCTGCG
    CGGGAGCTGT CATTTAAGAA GGGGGCATCT CTGCTGCTGT ACCAGAGAGC ATCCGACGAT
    TGGTGGGAGG GCAGACACAA TGGCATAGAT GGACTCGTGC CTCACCAGTA CATTGTTGTT
    CAGGATATGG ACGACACGTT CTCAGACACT TTGAGTCAGA AAGCGGACAG TGAGGCCAGC
    AGTGGACATG GAGGAGAGGA GAAGTGCTCC AGCAGAGACA TGGGCTCTCC GACAGACAGC
    AGACTGCCTG ATGCCTACAT TAGCAGGCAC AGAAAGAGAA GTGACCCTCC AACCCGCAGA
    CCTCCGGCTC GCCCAACTGA CACCCACTGC CTCGTCCACC CATCTCATCA CAGCAGCCAT
    ACCAACCCAG ATCTGGGCTC TCCGGTGATG GGCCACTACA GCCCGCGGGA CATGCTGAGG
    GGCCGAGGAC ACATGCCCAT GGACAGTCCA GAGAGACGAC GGCGAACAGG CCATGGCAGC
    CTGACCAACA TCAGCCGCCA CGAGTCCATT AAGAAGATGG AGAGTCCACC CATCCGCCGG
    TCCACCTCTT CAGGACAGTA CAGCAGCTTC AACGAGCCTC ATGTCAAGAG TCTGGACCCC
    GAGAGCATCG CACAGGACAT AGAGGAGACG ATGAACACTG CTCTGAATGA GCTGAAGGAA
    CTGGAGCGCC AGAGCTCTGC CAAACACGCC CCAGACGTGG TGCTGGACAC CCTGGAGCAG
    ATGAAGAACG CTCCCACCCC GGCCAGCTCC ACTGAGTCCC TCAGCCAACT CCACGGCCTC
    CTGCTGCGGC CTGCTGGCAC TGAGCTACAC ATGCGCCGCA GCACCAGCTC CTCCAGCGAC
    ACCATGAGCA CCTTCAAGCC TGCGGTGGCG CCTCGTATGG GGGTACAGCT CAAACCGCCC
    ACCCTGAGGC CCAAACCCAT GGTGCCGGTG CCCAAAACTG GAGCGGCGCA GCATCCGGCA
    GCTCCGCCAC AGGATCCATT GGACAAATCC TGCACCATGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55888
    Clone ID Related Accession (Same CDS sequence) XM_017352031.1 , XM_017352031.2
    Accession Version XM_017352031.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product SLIT-ROBO Rho GTPase-activating protein 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352031.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTGAAAA TGTCGAATCC TCCTGTGAAG TTCAAAAAGG ATAAAGAGAT CATTGTAGAG 
    TACGAGACTC AAGTCAAAGA GATTCGAGCT CAACTGGTGG AGCAGCAGAA ATGTCTAGAG
    CAGCAGACCG ACATGAGGGT CCAGCTCCTT CAGGACCTCC AGGATTTTTT CCGCAAGAAG
    TCTGAGATCG AGATGGAGTA TTCACGAAAC CTGGAGAAAC TCGCCGAACG CTTCATGGCC
    AAGACAAGGA GCACCAAGGA CCACCAGCAA TATAAGAAAG ACCAGAATCT GCTGTCCCCG
    GTGAACTGCT GGTACCTGTT GCTGAACCAG GTCCGACGAG AGAGCAAAGA CCACGCAACC
    CTGAGTGACA TTTATCTGAA TAATGTCATC ATGAGATTCA TGCAGATCAG TGAAGACTCC
    ACACGTCTTA TGAAAAAGAG TAAAGAAATC ACCTTTCAGC TGCAAGAAGA TTTGATGAAA
    GTCCTCAACG AGCTCTACAC GGTGATGAAG ACCTATCATA TGTATCACAC AGAGAGCATC
    AGTGCTGAGG GTAAACTGAA GGAGGCTGAG AAGCAGGAGG AGAAGCAGAT CGGCCGCGTT
    GGAGATCCAG TTTTCCACAT CCGGCTGGAG GACAGACACC AGAGACGCAG CTCCGTCAAG
    AAGATCGAGA AGATGAAGGA GAAGAGGCAG GCGAAGTATT CGGAGAACAA GCTGAAGTCG
    ATCAAAGCTC GGAACGAATA CCTGCTGACG CTGGAAGCCA CTAATGCTTC AATCTTCAAG
    TATTACATCC ACGACTTGTC TGATTTAATA GACTGTTGTG ATCTGGGATA TCATGCCTCT
    CTGAACCGGG CATTGCGGAC CTACCTCTCA GCCGAATATA ACCTGGAGAC GTCCCGTCAC
    GAGGGCCTGG ACATCATAGA GAATGCAGTG GACAGTCTGG ACCCCCGTAG TGACCGCCAG
    CGCTTCATGG AAATGTACCC GACCGCCTTC TGCCCTCCCG TCAAATTTGA GTTTCAGGCA
    CATATGGGAG ACGAGGTTTG TCAGATCACG GCACAGCCTC CTGTTCAGGC TGAGCTCCTC
    CTGAGGTTCC AGCAGCTTCA ATCCCGCCTC ACCACACTGA AGATCGAGAA CGAAGAGATC
    AAGAAGACGT CTGAGGCGAC ACTTACTACC ATCCAGGACA TGGTGACGGT TGAGGATTAT
    GACGTATCTG AATGTTTCCA TCATAGCCGC TCAACTGAAT CAGTTAAATC TACCGTGTCT
    GAAACCTACC TCAGCAAGCC GAGCATCGCC AAGAGACGCG CCAACCAGCA GGAGACTGAG
    CAGTTCTATT TTATGAAGTT TCGCGAGTAT CTGGAGGGCA GCAATCTCAT CACTAAACTC
    CAGGCAAAGC ATGATCTACT GAAGAGAACC CTCGGTGACG GTTACCGGCC TGAATATGCA
    ACTACAAGTC GTGGACGAAG GAACTCGCAC GCAAGACATC AGGACTCTGG TCAAGCAATA
    CCTCGAGTTG TTGAGAGCTG TATTCGATTC ATCAACCTGT ATGGCCTCCA ACATCAAGGA
    ATATTCAGAG TGCCTGGATC TCAAGTTGAA GTCAATGATA TCAAGAATTC ATTTGAGAGA
    GGGAACGATC CACTGACAGA CGAAGAGAAT AACCACGATA TAAACTCTGT CGCTGGCGTT
    TTGAAACTCT ACTTTCGGGG CTTGGAGAAC CCTCTGTTTC CAAAGGAAAG ATTCAATGAT
    CTCATCTCCT GTATCAGAAT AGAAAATCTG TATGAAAGAG CGTTGTATAT TCGAAAGATC
    CTTCTGACTG CACCCAGATC AGTGCTTGTG GTCATGCGAT ATCTGTTCGC CTTCCTCAAC
    CACTTGTCCC AGTACAGTGA TGAAAACATG ATGGACCCCT ATAATCTGGC CATCTGCTTT
    GGGCCGACCC TGATGCCCAC TCCCGAAACC CAGGACCAGG TGTCCTGCCA GGCCCACGTC
    AATGAGGTCA TCAAGACCAT CATCATCCAC CACGAAACCA TCTTTCCTGA CTCCAAGGAG
    CTAGACGGCC CCCTGTATGA GAAGTGTATG GCAGGTGGAG ATTATTGTGA GAGTCCATAC
    AGTGAACATG GAGCTCTTGA AGAGGTTGAT CAGGATGGAG GAGCAGAGAC CCACACCAGT
    GAGGATGAGG GAGAGCCCAT TGAAGCCATC GCCAAGTTCG ATTACGTGGG CCGCTCTGCG
    CGGGAGCTGT CATTTAAGAA GGGGGCATCT CTGCTGCTGT ACCAGAGAGC ATCCGACGAT
    TGGTGGGAGG GCAGACACAA TGGCATAGAT GGACTCGTGC CTCACCAGTA CATTGTTGTT
    CAGGATATGG ACGACACGTT CTCAGACACT TTGAGTCAGA AAGCGGACAG TGAGGCCAGC
    AGTGGACATG GAGGAGAGGA GAAGTGCTCC AGCAGAGACA TGGGCTCTCC GACAGACAGC
    AGACTGCCTG ATGCCTACAT TAGCAGGCAC AGAAAGAGAA GTGACCCTCC AACCCGCAGA
    CCTCCGGCTC GCCCAACTGA CACCCACTGC CTCGTCCACC CATCTCATCA CAGCAGCCAT
    ACCAACCCAG ATCTGGGCTC TCCGGTGATG GGCCACTACA GCCCGCGGGA CATGCTGAGG
    GGCCGAGGAC ACATGCCCAT GGACAGTCCA GAGAGACGAC GGCGAACAGG CCATGGCAGC
    CTGACCAACA TCAGCCGCCA CGAGTCCATT AAGAAGATGG AGAGTCCACC CATCCGCCGG
    TCCACCTCTT CAGGACAGTA CAGCAGCTTC AACGAGCCTC ATGTCAAGAG TCTGGACCCC
    GAGAGCATCG CACAGGACAT AGAGGAGACG ATGAACACTG CTCTGAATGA GCTGAAGGAA
    CTGGAGCGCC AGAGCTCTGC CAAACACGCC CCAGACGTGG TGCTGGACAC CCTGGAGCAG
    ATGAAGAACG CTCCCACCCC GGCCAGCTCC ACTGAGTCCC TCAGCCAACT CCACGGCCTC
    CTGCTGCGGC CTGCTGGCAC TGAGCTACAC ATGCGCCGCA GCACCAGCTC CTCCAGCGAC
    ACCATGAGCA CCTTCAAGCC TGCGGTGGCG CCTCGTATGG GGGTACAGCT CAAACCGCCC
    ACCCTGAGGC CCAAACCCAT GGTGCCGGTG CCCAAAACTG GAGCGGCGCA GCATCCGGCA
    GCTCCGCCAC AGGATCCATT GGACAAATCC TGCACCATGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207520.1
    CDS874..4095
    Translation

    Target ORF information:

    RefSeq Version XM_017352031.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio SLIT-ROBO Rho GTPase activating protein 1a (srgap1a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352031.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTTGAAAA TGTCGAATCC TCCTGTGAAG TTCAAAAAGG ATAAAGAGAT CATTGTAGAG 
    TACGAGACTC AAGTCAAAGA GATTCGAGCT CAACTGGTGG AGCAGCAGAA ATGTCTAGAG
    CAGCAGACCG ACATGAGGGT CCAGCTCCTT CAGGACCTCC AGGATTTTTT CCGCAAGAAG
    TCTGAGATCG AGATGGAGTA TTCACGAAAC CTGGAGAAAC TCGCCGAACG CTTCATGGCC
    AAGACAAGGA GCACCAAGGA CCACCAGCAA TATAAGAAAG ACCAGAATCT GCTGTCCCCG
    GTGAACTGCT GGTACCTGTT GCTGAACCAG GTCCGACGAG AGAGCAAAGA CCACGCAACC
    CTGAGTGACA TTTATCTGAA TAATGTCATC ATGAGATTCA TGCAGATCAG TGAAGACTCC
    ACACGTCTTA TGAAAAAGAG TAAAGAAATC ACCTTTCAGC TGCAAGAAGA TTTGATGAAA
    GTCCTCAACG AGCTCTACAC GGTGATGAAG ACCTATCATA TGTATCACAC AGAGAGCATC
    AGTGCTGAGG GTAAACTGAA GGAGGCTGAG AAGCAGGAGG AGAAGCAGAT CGGCCGCGTT
    GGAGATCCAG TTTTCCACAT CCGGCTGGAG GACAGACACC AGAGACGCAG CTCCGTCAAG
    AAGATCGAGA AGATGAAGGA GAAGAGGCAG GCGAAGTATT CGGAGAACAA GCTGAAGTCG
    ATCAAAGCTC GGAACGAATA CCTGCTGACG CTGGAAGCCA CTAATGCTTC AATCTTCAAG
    TATTACATCC ACGACTTGTC TGATTTAATA GACTGTTGTG ATCTGGGATA TCATGCCTCT
    CTGAACCGGG CATTGCGGAC CTACCTCTCA GCCGAATATA ACCTGGAGAC GTCCCGTCAC
    GAGGGCCTGG ACATCATAGA GAATGCAGTG GACAGTCTGG ACCCCCGTAG TGACCGCCAG
    CGCTTCATGG AAATGTACCC GACCGCCTTC TGCCCTCCCG TCAAATTTGA GTTTCAGGCA
    CATATGGGAG ACGAGGTTTG TCAGATCACG GCACAGCCTC CTGTTCAGGC TGAGCTCCTC
    CTGAGGTTCC AGCAGCTTCA ATCCCGCCTC ACCACACTGA AGATCGAGAA CGAAGAGATC
    AAGAAGACGT CTGAGGCGAC ACTTACTACC ATCCAGGACA TGGTGACGGT TGAGGATTAT
    GACGTATCTG AATGTTTCCA TCATAGCCGC TCAACTGAAT CAGTTAAATC TACCGTGTCT
    GAAACCTACC TCAGCAAGCC GAGCATCGCC AAGAGACGCG CCAACCAGCA GGAGACTGAG
    CAGTTCTATT TTATGAAGTT TCGCGAGTAT CTGGAGGGCA GCAATCTCAT CACTAAACTC
    CAGGCAAAGC ATGATCTACT GAAGAGAACC CTCGGTGACG GTTACCGGCC TGAATATGCA
    ACTACAAGTC GTGGACGAAG GAACTCGCAC GCAAGACATC AGGACTCTGG TCAAGCAATA
    CCTCGAGTTG TTGAGAGCTG TATTCGATTC ATCAACCTGT ATGGCCTCCA ACATCAAGGA
    ATATTCAGAG TGCCTGGATC TCAAGTTGAA GTCAATGATA TCAAGAATTC ATTTGAGAGA
    GGGAACGATC CACTGACAGA CGAAGAGAAT AACCACGATA TAAACTCTGT CGCTGGCGTT
    TTGAAACTCT ACTTTCGGGG CTTGGAGAAC CCTCTGTTTC CAAAGGAAAG ATTCAATGAT
    CTCATCTCCT GTATCAGAAT AGAAAATCTG TATGAAAGAG CGTTGTATAT TCGAAAGATC
    CTTCTGACTG CACCCAGATC AGTGCTTGTG GTCATGCGAT ATCTGTTCGC CTTCCTCAAC
    CACTTGTCCC AGTACAGTGA TGAAAACATG ATGGACCCCT ATAATCTGGC CATCTGCTTT
    GGGCCGACCC TGATGCCCAC TCCCGAAACC CAGGACCAGG TGTCCTGCCA GGCCCACGTC
    AATGAGGTCA TCAAGACCAT CATCATCCAC CACGAAACCA TCTTTCCTGA CTCCAAGGAG
    CTAGACGGCC CCCTGTATGA GAAGTGTATG GCAGGTGGAG ATTATTGTGA GAGTCCATAC
    AGTGAACATG GAGCTCTTGA AGAGGTTGAT CAGGATGGAG GAGCAGAGAC CCACACCAGT
    GAGGATGAGG GAGAGCCCAT TGAAGCCATC GCCAAGTTCG ATTACGTGGG CCGCTCTGCG
    CGGGAGCTGT CATTTAAGAA GGGGGCATCT CTGCTGCTGT ACCAGAGAGC ATCCGACGAT
    TGGTGGGAGG GCAGACACAA TGGCATAGAT GGACTCGTGC CTCACCAGTA CATTGTTGTT
    CAGGATATGG ACGACACGTT CTCAGACACT TTGAGTCAGA AAGCGGACAG TGAGGCCAGC
    AGTGGACATG GAGGAGAGGA GAAGTGCTCC AGCAGAGACA TGGGCTCTCC GACAGACAGC
    AGACTGCCTG ATGCCTACAT TAGCAGGCAC AGAAAGAGAA GTGACCCTCC AACCCGCAGA
    CCTCCGGCTC GCCCAACTGA CACCCACTGC CTCGTCCACC CATCTCATCA CAGCAGCCAT
    ACCAACCCAG ATCTGGGCTC TCCGGTGATG GGCCACTACA GCCCGCGGGA CATGCTGAGG
    GGCCGAGGAC ACATGCCCAT GGACAGTCCA GAGAGACGAC GGCGAACAGG CCATGGCAGC
    CTGACCAACA TCAGCCGCCA CGAGTCCATT AAGAAGATGG AGAGTCCACC CATCCGCCGG
    TCCACCTCTT CAGGACAGTA CAGCAGCTTC AACGAGCCTC ATGTCAAGAG TCTGGACCCC
    GAGAGCATCG CACAGGACAT AGAGGAGACG ATGAACACTG CTCTGAATGA GCTGAAGGAA
    CTGGAGCGCC AGAGCTCTGC CAAACACGCC CCAGACGTGG TGCTGGACAC CCTGGAGCAG
    ATGAAGAACG CTCCCACCCC GGCCAGCTCC ACTGAGTCCC TCAGCCAACT CCACGGCCTC
    CTGCTGCGGC CTGCTGGCAC TGAGCTACAC ATGCGCCGCA GCACCAGCTC CTCCAGCGAC
    ACCATGAGCA CCTTCAAGCC TGCGGTGGCG CCTCGTATGG GGGTACAGCT CAAACCGCCC
    ACCCTGAGGC CCAAACCCAT GGTGCCGGTG CCCAAAACTG GAGCGGCGCA GCATCCGGCA
    GCTCCGCCAC AGGATCCATT GGACAAATCC TGCACCATGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.