gpr158a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gpr158a
Entrez Gene ID	570001
Full Name	G protein-coupled receptor 158a
Synonyms	gpr158,sb:eu1014
General protein information	Preferred Names G protein-coupled receptor 158a Names probable G-protein coupled receptor 158 G protein-coupled receptor 158
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005162535.4	XP_005162592.1	probable G-protein coupled receptor 158 isoform X2
XM_005162535.3	XP_005162592.1	probable G-protein coupled receptor 158 isoform X2
XM_005162534.3	XP_005162591.1	probable G-protein coupled receptor 158 isoform X1
XM_005162534.4	XP_005162591.1	probable G-protein coupled receptor 158 isoform X1
XM_021470264.1	XP_021325939.1	probable G-protein coupled receptor 158 isoform X3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Gpr158	NP_001004761.1
Rattus norvegicus (Norway rat)	Gpr158	NP_001163797.1
Danio rerio (zebrafish)	LOC570001	XP_005162592.1
Canis lupus familiaris (dog)	GPR158	XP_535168.3
Bos taurus (cattle)	GPR158	NP_001193371.1
Gallus gallus (chicken)	GPR158	NP_001244228.1
	GPR158	XP_001101165.1
Xenopus tropicalis (tropical clawed frog)	gpr158	XP_002933190.2
Homo sapiens (human)	GPR158	NP_065803.2
Pan troglodytes (chimpanzee)	GPR158	XP_521427.2
Danio rerio (zebrafish)	LOC568637	XP_697068.4

Gene Ontology

The following gpr158a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gpr158a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa151762	XM_021470264.1 Latest version!	Danio rerio G protein-coupled receptor 158a (gpr158a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa28308	XM_005162534.4 Latest version!	Danio rerio G protein-coupled receptor 158a (gpr158a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa28309	XM_005162535.4 Latest version!	Danio rerio G protein-coupled receptor 158a (gpr158a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa28309	XM_005162535.3	Danio rerio G protein-coupled receptor 158a (gpr158a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa28308	XM_005162534.3	Danio rerio G protein-coupled receptor 158a (gpr158a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gpr158a ( XM_021470264.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325939.1 Danio rerio G protein-coupled receptor 158a (gpr158a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa151762
Clone ID Related Accession (Same CDS sequence)	XM_021470264.1
Accession Version	XM_021470264.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2148bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	probable G-protein coupled receptor 158 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATAA TCCGGATATC GTTACTCTTG CAAGTGGGAT TTGTTATAGG ATCAAATTAT 
AGATATGTGG AACATGACCG GGACCTGAAA GGAAAGCTGC CCATTTACAA CACGCACAGC 
AACCAGCACG CGCACCTCCG GAAGCCCCAT TCGGGTTTAG TGCAGAAGAT CGAGGAGCAG 
GTACCCAAAG TAGTGACTGC GTTTCTGCAC ACGGGGGATT CCTCCACCTT AAAGCACGCC 
AACTGCTCCA GGAGATATGA GCTGGCGGTG TTGCGGGGAA GGACGCGCGG AGACGCGCAT 
CACTCCATGC GCAGCGTCCT GGACACGGTC CTTCACGCCA CAAACTTCCT CAACATGATA 
TTACAAACAA ACCGATCCAG AAATCACAGC CCCAGACATG CAGACATGGA GTGGTACAAC 
GCGCTGGTCA GGAGTATCCT GGAGGGAGAC CTCAAGATCC ACCGAGCGGT GGTCACCTTG 
AGCACAGAAT CCCCTCCTGA AGGACCACCT GTTTACCTTC AGGCTACACG GGCAGGTGGA 
GAGATCATTC TGCAGGATTT ATCCAACATT ACCCATCGGA TGTTGAGAAA CAGAACGGCT 
GACACCGAGT GGTATCATGA GCACAAACAT AAAAAGAAGA CACATCTGCA AAAACGAGTG 
CTGAGTCAAG ATTTTGCCTC GTTTGACGCG TCCGTGCGCA GTGGAGAAAG TTATATTACC 
GAAAAAACTC AGATACACTG GTCAGCTCCG TACTTGGAGT GCGAGAATGG GAATTTCATA 
CCCAGATGGC TCTTGACACT GTCAGCTGGC TTCTATGGCC TTAAACCCAA CAGGAACCCA 
GACTTCAGGG GTGTTGTTCG AGTGGACGTC AGCCTGCAGG ATGTGGATAT TGACCAGTGC 
TCTGCAGACG GCTGGTTTGC AGGAACACAC AGGTGTAACA TCACCACTAT GGAGTGTTTG 
CCGGTTCCTG GGCATGGATT TGTGCTGGAC AAGTATAAAT GTCACTGCAA AAGTGGCTAT 
TATCATCCAA GCAGAGTTGC AGTGAATGGC TTTAAAAGGA AAGCAGGAGG GAAGGGGACC 
GAGGGTTTGT CCCATAACAG GGATGTTCCA GAAACTTCCA GCCACTGTCT GCCCTGCCAG 
GAAGGCTGTT CTTTCTGCAA GGACGATACG CCCTGCGTGG CCCGGGGTGA TGGAGCCCTG 
CGTATGGCGG TGCTCTCCTT CCAGGGCCTG TGCATGCTGG TGGATTTCAT CAGTATGGTG 
CTGCTTTACC ATTTCCGCAG GAACAAGAGT ATCAGAGCAT CAGGACTCAT TCTGCTGGAG 
GCCATCTTGT TTGGAGCTCT TCTCCTGTAC TTTCCGGTGG TGATCCTGTA CTTCCAGCCA 
AGTGTTTTCC GCTGTATCCT GCTGCGATGG GTCCGTCTGC TCGGGTTTGC CACTGTATAC 
GGAACAGTCA CTCTCAAGCT CTACAGGGTC CTCAAGGTGT TCCTGTCACG TACAGCCCAG 
CGGATCCCTT ACATGACCAG CTGGAGAGTG TTGCGTCTAC TGTGTATCAT CCTGTTGATC 
GTGTTATGGT TCGCAATAGC CTGGACCTCC GCTGTTTGTC AGAATCCAAA CAGGCACCTC 
GCTCTCATCA GTGTGGGCTT CACCACTGAC GGACTGCAGT TCAGCATGTG TCTGCTGGAC 
CGCTGGGACT ACATGATTGC TGTGGCTGAG TTCCTGTTTC TTCTTTGGGG TGTATATCTT 
TGCTTTGCGG TGAGGACCGT CCCATCGGCA TATCACGAAC CACGCTACAT GGCCATCGCA 
GTTCACAATG AGCTCATCCT CTCGGCCATA TTCCACATTT TAAGGTTCAC ACTTGCTCCT 
GAATTGCACC CAGACTGGAT GCTGATGCTT TTCTTTGCTC ATACACACTT AACGGTGACT 
GTCACTTTGG GGTTGCTGCT TGTGCCAAAG TTCTTGTTTG CGGGCACACA TCTAAGGGAT 
GACATTGCTA CAGAAGCTTA CGAAGATGAA TTGGACATGG GGCGGTCTGG GTCATACCTG 
AATAGCAGTA TCACATCTGC TTGGAGTGAG CACAGTCTGG ACCCAGAGGA TATCCGGGTA 
GAGCTGAAGA AGTCTCTCAC TGAAGACAAA TTTTGGGCAT TTTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325939.1
CDS	1999..4146
Translation	MAIIRISLLLQVGFVIGSNYRYVEHDRDLKGKLPIYNTHSNQHAHLRKPHSGLVQKIEEQVPKVVTAFLHTGDSSTLKHANCSRRYELAVLRGRTRGDAHHSMRSVLDTVLHATNFLNMILQTNRSRNHSPRHADMEWYNALVRSILEGDLKIHRAVVTLSTESPPEGPPVYLQATRAGGEIILQDLSNITHRMLRNRTADTEWYHEHKHKKKTHLQKRVLSQDFASFDASVRSGESYITEKTQIHWSAPYLECENGNFIPRWLLTLSAGFYGLKPNRNPDFRGVVRVDVSLQDVDIDQCSADGWFAGTHRCNITTMECLPVPGHGFVLDKYKCHCKSGYYHPSRVAVNGFKRKAGGKGTEGLSHNRDVPETSSHCLPCQEGCSFCKDDTPCVARGDGALRMAVLSFQGLCMLVDFISMVLLYHFRRNKSIRASGLILLEAILFGALLLYFPVVILYFQPSVFRCILLRWVRLLGFATVYGTVTLKLYRVLKVFLSRTAQRIPYMTSWRVLRLLCIILLIVLWFAIAWTSAVCQNPNRHLALISVGFTTDGLQFSMCLLDRWDYMIAVAEFLFLLWGVYLCFAVRTVPSAYHEPRYMAIAVHNELILSAIFHILRFTLAPELHPDWMLMLFFAHTHLTVTVTLGLLLVPKFLFAGTHLRDDIATEAYEDELDMGRSGSYLNSSITSAWSEHSLDPEDIRVELKKSLTEDKFWAFC

Target ORF information:

RefSeq Version	XM_021470264.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio G protein-coupled receptor 158a (gpr158a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470264.1

ORF Insert Sequence:

ATGGCCATAA TCCGGATATC GTTACTCTTG CAAGTGGGAT TTGTTATAGG ATCAAATTAT 
AGATATGTGG AACATGACCG GGACCTGAAA GGAAAGCTGC CCATTTACAA CACGCACAGC 
AACCAGCACG CGCACCTCCG GAAGCCCCAT TCGGGTTTAG TGCAGAAGAT CGAGGAGCAG 
GTACCCAAAG TAGTGACTGC GTTTCTGCAC ACGGGGGATT CCTCCACCTT AAAGCACGCC 
AACTGCTCCA GGAGATATGA GCTGGCGGTG TTGCGGGGAA GGACGCGCGG AGACGCGCAT 
CACTCCATGC GCAGCGTCCT GGACACGGTC CTTCACGCCA CAAACTTCCT CAACATGATA 
TTACAAACAA ACCGATCCAG AAATCACAGC CCCAGACATG CAGACATGGA GTGGTACAAC 
GCGCTGGTCA GGAGTATCCT GGAGGGAGAC CTCAAGATCC ACCGAGCGGT GGTCACCTTG 
AGCACAGAAT CCCCTCCTGA AGGACCACCT GTTTACCTTC AGGCTACACG GGCAGGTGGA 
GAGATCATTC TGCAGGATTT ATCCAACATT ACCCATCGGA TGTTGAGAAA CAGAACGGCT 
GACACCGAGT GGTATCATGA GCACAAACAT AAAAAGAAGA CACATCTGCA AAAACGAGTG 
CTGAGTCAAG ATTTTGCCTC GTTTGACGCG TCCGTGCGCA GTGGAGAAAG TTATATTACC 
GAAAAAACTC AGATACACTG GTCAGCTCCG TACTTGGAGT GCGAGAATGG GAATTTCATA 
CCCAGATGGC TCTTGACACT GTCAGCTGGC TTCTATGGCC TTAAACCCAA CAGGAACCCA 
GACTTCAGGG GTGTTGTTCG AGTGGACGTC AGCCTGCAGG ATGTGGATAT TGACCAGTGC 
TCTGCAGACG GCTGGTTTGC AGGAACACAC AGGTGTAACA TCACCACTAT GGAGTGTTTG 
CCGGTTCCTG GGCATGGATT TGTGCTGGAC AAGTATAAAT GTCACTGCAA AAGTGGCTAT 
TATCATCCAA GCAGAGTTGC AGTGAATGGC TTTAAAAGGA AAGCAGGAGG GAAGGGGACC 
GAGGGTTTGT CCCATAACAG GGATGTTCCA GAAACTTCCA GCCACTGTCT GCCCTGCCAG 
GAAGGCTGTT CTTTCTGCAA GGACGATACG CCCTGCGTGG CCCGGGGTGA TGGAGCCCTG 
CGTATGGCGG TGCTCTCCTT CCAGGGCCTG TGCATGCTGG TGGATTTCAT CAGTATGGTG 
CTGCTTTACC ATTTCCGCAG GAACAAGAGT ATCAGAGCAT CAGGACTCAT TCTGCTGGAG 
GCCATCTTGT TTGGAGCTCT TCTCCTGTAC TTTCCGGTGG TGATCCTGTA CTTCCAGCCA 
AGTGTTTTCC GCTGTATCCT GCTGCGATGG GTCCGTCTGC TCGGGTTTGC CACTGTATAC 
GGAACAGTCA CTCTCAAGCT CTACAGGGTC CTCAAGGTGT TCCTGTCACG TACAGCCCAG 
CGGATCCCTT ACATGACCAG CTGGAGAGTG TTGCGTCTAC TGTGTATCAT CCTGTTGATC 
GTGTTATGGT TCGCAATAGC CTGGACCTCC GCTGTTTGTC AGAATCCAAA CAGGCACCTC 
GCTCTCATCA GTGTGGGCTT CACCACTGAC GGACTGCAGT TCAGCATGTG TCTGCTGGAC 
CGCTGGGACT ACATGATTGC TGTGGCTGAG TTCCTGTTTC TTCTTTGGGG TGTATATCTT 
TGCTTTGCGG TGAGGACCGT CCCATCGGCA TATCACGAAC CACGCTACAT GGCCATCGCA 
GTTCACAATG AGCTCATCCT CTCGGCCATA TTCCACATTT TAAGGTTCAC ACTTGCTCCT 
GAATTGCACC CAGACTGGAT GCTGATGCTT TTCTTTGCTC ATACACACTT AACGGTGACT 
GTCACTTTGG GGTTGCTGCT TGTGCCAAAG TTCTTGTTTG CGGGCACACA TCTAAGGGAT 
GACATTGCTA CAGAAGCTTA CGAAGATGAA TTGGACATGG GGCGGTCTGG GTCATACCTG 
AATAGCAGTA TCACATCTGC TTGGAGTGAG CACAGTCTGG ACCCAGAGGA TATCCGGGTA 
GAGCTGAAGA AGTCTCTCAC TGAAGACAAA TTTTGGGCAT TTTGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gpr158a ( XM_005162534.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162591.1 Danio rerio G protein-coupled receptor 158a (gpr158a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa28308
Clone ID Related Accession (Same CDS sequence)	XM_005162534.3 , XM_005162534.4
Accession Version	XM_005162534.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	probable G-protein coupled receptor 158 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162534.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATAA TCCGGATATC GTTACTCTTG CAAGTGGGAT TTGTTATAGG ATCAAATTAT 
AGATATGTGG AACATGACCG GGACCTGAAA GGAAAGCTGC CCATTTACAA CACGCACAGC 
AACCAGCACG CGCACCTCCG GAAGCCCCAT TCGGGTTTAG TGCAGAAGAT CGAGGAGCAG 
GTACCCAAAG TAGTGACTGC GTTTCTGCAC ACGGGGGATT CCTCCACCTT AAAGCACGCC 
AACTGCTCCA GGAGATATGA GCTGGCGGTG TTGCGGGGAA GGACGCGCGG AGACGCGCAT 
CACTCCATGC GCAGCGTCCT GGACACGGTC CTTCACGCCA CAAACTTCCT CAACATGATA 
TTACAAACAA ACCGATCCAG AAATCACAGC CCCAGACATG CAGACATGGA GTGGTACAAC 
GCGCTGGTCA GGAGTATCCT GGAGGGAGAC CTCAAGATCC ACCGAGCGGT GGTCACCTTG 
AGCACAGAAT CCCCTCCTGA AGGACCACCT GTTTACCTTC AGGCTACACG GGCAGGTGGA 
GAGATCATTC TGCAGGATTT ATCCAACATT ACCCATCGGA TGTTGAGAAA CAGAACGGCT 
GACACCGAGT GGTATCATGA GCACAAACAT AAAAAGAAGA CACATCTGCA AAAACGAGTG 
CTGAGTCAAG ATTTTGCCTC GTTTGACGCG TCCGTGCGCA GTGGAGAAAG TTATATTACC 
GAAAAAACTC AGATACACTG GTCAGCTCCG TACTTGGAGT GCGAGAATGG GAATTTCATA 
CCCAGATGGC TCTTGACACT GTCAGCTGGC TTCTATGGCC TTAAACCCAA CAGGAACCCA 
GACTTCAGGG GTGTTGTTCG AGTGGACGTC AGCCTGCAGG ATGTGGATAT TGACCAGTGC 
TCTGCAGACG GCTGGTTTGC AGGAACACAC AGGTGTAACA TCACCACTAT GGAGTGTTTG 
CCGGTTCCTG GGCATGGATT TGTGCTGGAC AAGTATAAAT GTCACTGCAA AAGTGGCTAT 
TATCATCCAA GCAGAGTTGC AGTGAATGGC TTTAAAAGGA AAGCAGGAGG GAAGGGGACC 
GAGGGTTTGT CCCATAACAG GGATGTTCCA GAAACTTCCA GCCACTGTCT GCCCTGCCAG 
GAAGGCTGTT CTTTCTGCAA GGACGATACG CCCTGCGTGG CCCGGGGTGA TGGAGCCCTG 
CGTATGGCGG TGCTCTCCTT CCAGGGCCTG TGCATGCTGG TGGATTTCAT CAGTATGGTG 
CTGCTTTACC ATTTCCGCAG GAACAAGAGT ATCAGAGCAT CAGGACTCAT TCTGCTGGAG 
GCCATCTTGT TTGGAGCTCT TCTCCTGTAC TTTCCGGTGG TGATCCTGTA CTTCCAGCCA 
AGTGTTTTCC GCTGTATCCT GCTGCGATGG GTCCGTCTGC TCGGGTTTGC CACTGTATAC 
GGAACAGTCA CTCTCAAGCT CTACAGGGTC CTCAAGGTGT TCCTGTCACG TACAGCCCAG 
CGGATCCCTT ACATGACCAG CTGGAGAGTG TTGCGTCTAC TGTGTATCAT CCTGTTGATC 
GTGTTATGGT TCGCAATAGC CTGGACCTCC GCTGTTTGTC AGAATCCAAA CAGGCACCTC 
GCTCTCATCA GTGTGGGCTT CACCACTGAC GGACTGCAGT TCAGCATGTG TCTGCTGGAC 
CGCTGGGACT ACATGATTGC TGTGGCTGAG TTCCTGTTTC TTCTTTGGGG TGTATATCTT 
TGCTTTGCGG TGAGGACCGT CCCATCGGCA TATCACGAAC CACGCTACAT GGCCATCGCA 
GTTCACAATG AGCTCATCCT CTCGGCCATA TTCCACATTT TAAGGTTCAC ACTTGCTCCT 
GAATTGCACC CAGACTGGAT GCTGATGCTT TTCTTTGCTC ATACACACTT AACGGTGACT 
GTCACTTTGG GGTTGCTGCT TGTGCCAAAG TTCTTGTTTG CGGGCACACA TCTAAGGGAT 
GACATTGCTA CAGAAGCTTA CGAAGATGAA TTGGACATGG GGCGGTCTGG GTCATACCTG 
AATAGCAGTA TCACATCTGC TTGGAGTGAG CACAGTCTGG ACCCAGAGGA TATCCGGACA 
CCAGAGGAAA TGGGCCAGCT AAGTTCTATT AATGGCTGTG GCGTAAGGTC TTGCGACAGT 
CTCTGGGAAA AACGTACCAA CGAAGAGCTG AAGAAACTCT ACTCTCAACT GGAGATTTAC 
AAGAGGAAAA AGATGCTGGC TAACAATCCT CACCTGCAGA AGAAGCGAAG CTCCAAAAAG 
GGCCTAGGCC GCTCTCTCAT GCGACGCATC ACTGAGATCC CAGAGACAAT GGGACGCCAG 
TGTAGCCGGG ACGACAAAGA CCAAGGTGAA CACGGGAGCA ACCATAACAG CATCTGTGTG 
CTGAGAAAGA ATCCTTTTGA TCCCTCACAC TCTGTTAAGC CTGCTAAAGA TGAATCCTTA 
AAGAGCAAAG TTTTCTCCCT TAAAAAGTCC CACAGCAGTT ACGACCATGT TCGAGACCAC 
AGTGAAGATT CCAGCAGTTC GGCAACTGAC AAGATGGAAG TCACAACCAC AGAGGGCTCT 
CTTCTCGAAA CCCTAATGGG AAAAAAGCTG GTCAAAAAGT CTTCTGAAAA GATTGATGTA 
GCCAGTGAGT CAACAGAGTC GGTTCCCTTG GTGTGCAAGT CAGCCAGCGC TCACAATCTG 
ACAGCTGATA AGAAACCTAT TCACCCCAGA ACATCCATGC TGCAGAAATC TCTAAGCGTT 
ATTGCCAGTG CCAAAGAGAA AACTCTTGGT CTAACAGGAA AGGCCCAGGG TGTAGAGGAT 
TCAACAAAGA AAGCCAATCA AAAGAACAGT GAGGGCAAGA CCTCAGCTGA AGCTGAAGAA 
AAAGAAGGTT ATCCGAAGAT GATTGTCAGT CAATCAGTGG AATACAAGCA GACAGCAGCT 
AAAACGGGTA TAATGAAGCC ACAAATGAGC GGAAGCCAGC CCTCCATTTG CTCAGAAACA 
GCCAAGGGGA AAGACCTGTG TGACCTCTCA GAAGTGTGCC CTTGGGAAGT GGAGGATTTG 
CCAACTCCCT CCGAAAATAA AGTCCAGAAA CATGTGTCTA TTGCTCCTGA GCAAACAAAC 
ACAGTTCGTG GAGGTAGCAG CAAGACGACA AAGTCACAAC AGCAAAAGCA AAAAGGAGCA 
GAGCAGTCAC CTTCTGCGGC CAGGCATCCA AACAAGAAAG CTGCAGATAG AGCAGACATA 
TGTCCGTGGG AGGAAGGAAA CGAGGATCAT GGTCAGCCAA CAGGGCCAAA ACCTCACTCC 
AATACTAAGT CTGCCATGTC TCAACAACAG GCTTCCAGAG AGGTTGCCAA GGTTTTAAAA 
GCAGACGTCT GTCCATGGGA CTTTGAGGAA GTATCAAGCA AGCCAAAGGA CTCCCCTCGC 
TCCCCTGATC AGAGCAGACC AAAAAAGGGC ACCACACCAA CTGAGATCAA AGGCAAAAAA 
AGCTCCTCGC CTACTGAAGT CAAGGGCAAG GGTACTACGC CTACAGAGGT CAAAGCAAAG 
AGTATTCTCT CTGAACCTAC TAAATCCACA GGCAGCTCAC TGCAGCCGCC CTCTAAAGCA 
GACGTGTGTC CATGGGACTT TGAGACTATG TCAAGTCCCA CCTCTGAAAA GACCCCTTGT 
TCTTCTCAGG TTCCTAAATC AAAGGAGACC CATGCTATAA AGAAGGGAAA TTCCCCTGTC 
AGATTATTAG AGTTGGAAAG AGGAAACACA AAGGGTCCAG ATGAGGACAA AATGAAATCA 
AACACAAAGT TCAGCTCTGG CAAGACAAAA GAAAGACCCG TGAGCCAGTC TAAAATAATG 
GCCGAGGTTT GTCCGTGGGA TGTTGATAGT AGTCAGGGAA ATGTTTCAAT GGAAAAACAA 
AAAAGCCCAA ATGTTGCATT AGCAAAAAGC AAACAAGCAG ACGTATGTCC TTGGGATTTT 
GAAGAGGCAG CAACTAGTAA GGAGGCCAAC AGGGGCACCG GTGGCAAACA GAAAAGTTCA 
AATTCTAGTG GTGTTAAAAC GGCAGATGTC TGTCCATGGG ACTTTGATGA TCAAGCATCG 
ACAAAAAAGG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162591.1
CDS	876..4970
Translation	MAIIRISLLLQVGFVIGSNYRYVEHDRDLKGKLPIYNTHSNQHAHLRKPHSGLVQKIEEQVPKVVTAFLHTGDSSTLKHANCSRRYELAVLRGRTRGDAHHSMRSVLDTVLHATNFLNMILQTNRSRNHSPRHADMEWYNALVRSILEGDLKIHRAVVTLSTESPPEGPPVYLQATRAGGEIILQDLSNITHRMLRNRTADTEWYHEHKHKKKTHLQKRVLSQDFASFDASVRSGESYITEKTQIHWSAPYLECENGNFIPRWLLTLSAGFYGLKPNRNPDFRGVVRVDVSLQDVDIDQCSADGWFAGTHRCNITTMECLPVPGHGFVLDKYKCHCKSGYYHPSRVAVNGFKRKAGGKGTEGLSHNRDVPETSSHCLPCQEGCSFCKDDTPCVARGDGALRMAVLSFQGLCMLVDFISMVLLYHFRRNKSIRASGLILLEAILFGALLLYFPVVILYFQPSVFRCILLRWVRLLGFATVYGTVTLKLYRVLKVFLSRTAQRIPYMTSWRVLRLLCIILLIVLWFAIAWTSAVCQNPNRHLALISVGFTTDGLQFSMCLLDRWDYMIAVAEFLFLLWGVYLCFAVRTVPSAYHEPRYMAIAVHNELILSAIFHILRFTLAPELHPDWMLMLFFAHTHLTVTVTLGLLLVPKFLFAGTHLRDDIATEAYEDELDMGRSGSYLNSSITSAWSEHSLDPEDIRTPEEMGQLSSINGCGVRSCDSLWEKRTNEELKKLYSQLEIYKRKKMLANNPHLQKKRSSKKGLGRSLMRRITEIPETMGRQCSRDDKDQGEHGSNHNSICVLRKNPFDPSHSVKPAKDESLKSKVFSLKKSHSSYDHVRDHSEDSSSSATDKMEVTTTEGSLLETLMGKKLVKKSSEKIDVASESTESVPLVCKSASAHNLTADKKPIHPRTSMLQKSLSVIASAKEKTLGLTGKAQGVEDSTKKANQKNSEGKTSAEAEEKEGYPKMIVSQSVEYKQTAAKTGIMKPQMSGSQPSICSETAKGKDLCDLSEVCPWEVEDLPTPSENKVQKHVSIAPEQTNTVRGGSSKTTKSQQQKQKGAEQSPSAARHPNKKAADRADICPWEEGNEDHGQPTGPKPHSNTKSAMSQQQASREVAKVLKADVCPWDFEEVSSKPKDSPRSPDQSRPKKGTTPTEIKGKKSSSPTEVKGKGTTPTEVKAKSILSEPTKSTGSSLQPPSKADVCPWDFETMSSPTSEKTPCSSQVPKSKETHAIKKGNSPVRLLELERGNTKGPDEDKMKSNTKFSSGKTKERPVSQSKIMAEVCPWDVDSSQGNVSMEKQKSPNVALAKSKQADVCPWDFEEAATSKEANRGTGGKQKSSNSSGVKTADVCPWDFDDQASTKKA

Target ORF information:

RefSeq Version	XM_005162534.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio G protein-coupled receptor 158a (gpr158a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162534.3

ORF Insert Sequence:

ATGGCCATAA TCCGGATATC GTTACTCTTG CAAGTGGGAT TTGTTATAGG ATCAAATTAT 
AGATATGTGG AACATGACCG GGACCTGAAA GGAAAGCTGC CCATTTACAA CACGCACAGC 
AACCAGCACG CGCACCTCCG GAAGCCCCAT TCGGGTTTAG TGCAGAAGAT CGAGGAGCAG 
GTACCCAAAG TAGTGACTGC GTTTCTGCAC ACGGGGGATT CCTCCACCTT AAAGCACGCC 
AACTGCTCCA GGAGATATGA GCTGGCGGTG TTGCGGGGAA GGACGCGCGG AGACGCGCAT 
CACTCCATGC GCAGCGTCCT GGACACGGTC CTTCACGCCA CAAACTTCCT CAACATGATA 
TTACAAACAA ACCGATCCAG AAATCACAGC CCCAGACATG CAGACATGGA GTGGTACAAC 
GCGCTGGTCA GGAGTATCCT GGAGGGAGAC CTCAAGATCC ACCGAGCGGT GGTCACCTTG 
AGCACAGAAT CCCCTCCTGA AGGACCACCT GTTTACCTTC AGGCTACACG GGCAGGTGGA 
GAGATCATTC TGCAGGATTT ATCCAACATT ACCCATCGGA TGTTGAGAAA CAGAACGGCT 
GACACCGAGT GGTATCATGA GCACAAACAT AAAAAGAAGA CACATCTGCA AAAACGAGTG 
CTGAGTCAAG ATTTTGCCTC GTTTGACGCG TCCGTGCGCA GTGGAGAAAG TTATATTACC 
GAAAAAACTC AGATACACTG GTCAGCTCCG TACTTGGAGT GCGAGAATGG GAATTTCATA 
CCCAGATGGC TCTTGACACT GTCAGCTGGC TTCTATGGCC TTAAACCCAA CAGGAACCCA 
GACTTCAGGG GTGTTGTTCG AGTGGACGTC AGCCTGCAGG ATGTGGATAT TGACCAGTGC 
TCTGCAGACG GCTGGTTTGC AGGAACACAC AGGTGTAACA TCACCACTAT GGAGTGTTTG 
CCGGTTCCTG GGCATGGATT TGTGCTGGAC AAGTATAAAT GTCACTGCAA AAGTGGCTAT 
TATCATCCAA GCAGAGTTGC AGTGAATGGC TTTAAAAGGA AAGCAGGAGG GAAGGGGACC 
GAGGGTTTGT CCCATAACAG GGATGTTCCA GAAACTTCCA GCCACTGTCT GCCCTGCCAG 
GAAGGCTGTT CTTTCTGCAA GGACGATACG CCCTGCGTGG CCCGGGGTGA TGGAGCCCTG 
CGTATGGCGG TGCTCTCCTT CCAGGGCCTG TGCATGCTGG TGGATTTCAT CAGTATGGTG 
CTGCTTTACC ATTTCCGCAG GAACAAGAGT ATCAGAGCAT CAGGACTCAT TCTGCTGGAG 
GCCATCTTGT TTGGAGCTCT TCTCCTGTAC TTTCCGGTGG TGATCCTGTA CTTCCAGCCA 
AGTGTTTTCC GCTGTATCCT GCTGCGATGG GTCCGTCTGC TCGGGTTTGC CACTGTATAC 
GGAACAGTCA CTCTCAAGCT CTACAGGGTC CTCAAGGTGT TCCTGTCACG TACAGCCCAG 
CGGATCCCTT ACATGACCAG CTGGAGAGTG TTGCGTCTAC TGTGTATCAT CCTGTTGATC 
GTGTTATGGT TCGCAATAGC CTGGACCTCC GCTGTTTGTC AGAATCCAAA CAGGCACCTC 
GCTCTCATCA GTGTGGGCTT CACCACTGAC GGACTGCAGT TCAGCATGTG TCTGCTGGAC 
CGCTGGGACT ACATGATTGC TGTGGCTGAG TTCCTGTTTC TTCTTTGGGG TGTATATCTT 
TGCTTTGCGG TGAGGACCGT CCCATCGGCA TATCACGAAC CACGCTACAT GGCCATCGCA 
GTTCACAATG AGCTCATCCT CTCGGCCATA TTCCACATTT TAAGGTTCAC ACTTGCTCCT 
GAATTGCACC CAGACTGGAT GCTGATGCTT TTCTTTGCTC ATACACACTT AACGGTGACT 
GTCACTTTGG GGTTGCTGCT TGTGCCAAAG TTCTTGTTTG CGGGCACACA TCTAAGGGAT 
GACATTGCTA CAGAAGCTTA CGAAGATGAA TTGGACATGG GGCGGTCTGG GTCATACCTG 
AATAGCAGTA TCACATCTGC TTGGAGTGAG CACAGTCTGG ACCCAGAGGA TATCCGGACA 
CCAGAGGAAA TGGGCCAGCT AAGTTCTATT AATGGCTGTG GCGTAAGGTC TTGCGACAGT 
CTCTGGGAAA AACGTACCAA CGAAGAGCTG AAGAAACTCT ACTCTCAACT GGAGATTTAC 
AAGAGGAAAA AGATGCTGGC TAACAATCCT CACCTGCAGA AGAAGCGAAG CTCCAAAAAG 
GGCCTAGGCC GCTCTCTCAT GCGACGCATC ACTGAGATCC CAGAGACAAT GGGACGCCAG 
TGTAGCCGGG ACGACAAAGA CCAAGGTGAA CACGGGAGCA ACCATAACAG CATCTGTGTG 
CTGAGAAAGA ATCCTTTTGA TCCCTCACAC TCTGTTAAGC CTGCTAAAGA TGAATCCTTA 
AAGAGCAAAG TTTTCTCCCT TAAAAAGTCC CACAGCAGTT ACGACCATGT TCGAGACCAC 
AGTGAAGATT CCAGCAGTTC GGCAACTGAC AAGATGGAAG TCACAACCAC AGAGGGCTCT 
CTTCTCGAAA CCCTAATGGG AAAAAAGCTG GTCAAAAAGT CTTCTGAAAA GATTGATGTA 
GCCAGTGAGT CAACAGAGTC GGTTCCCTTG GTGTGCAAGT CAGCCAGCGC TCACAATCTG 
ACAGCTGATA AGAAACCTAT TCACCCCAGA ACATCCATGC TGCAGAAATC TCTAAGCGTT 
ATTGCCAGTG CCAAAGAGAA AACTCTTGGT CTAACAGGAA AGGCCCAGGG TGTAGAGGAT 
TCAACAAAGA AAGCCAATCA AAAGAACAGT GAGGGCAAGA CCTCAGCTGA AGCTGAAGAA 
AAAGAAGGTT ATCCGAAGAT GATTGTCAGT CAATCAGTGG AATACAAGCA GACAGCAGCT 
AAAACGGGTA TAATGAAGCC ACAAATGAGC GGAAGCCAGC CCTCCATTTG CTCAGAAACA 
GCCAAGGGGA AAGACCTGTG TGACCTCTCA GAAGTGTGCC CTTGGGAAGT GGAGGATTTG 
CCAACTCCCT CCGAAAATAA AGTCCAGAAA CATGTGTCTA TTGCTCCTGA GCAAACAAAC 
ACAGTTCGTG GAGGTAGCAG CAAGACGACA AAGTCACAAC AGCAAAAGCA AAAAGGAGCA 
GAGCAGTCAC CTTCTGCGGC CAGGCATCCA AACAAGAAAG CTGCAGATAG AGCAGACATA 
TGTCCGTGGG AGGAAGGAAA CGAGGATCAT GGTCAGCCAA CAGGGCCAAA ACCTCACTCC 
AATACTAAGT CTGCCATGTC TCAACAACAG GCTTCCAGAG AGGTTGCCAA GGTTTTAAAA 
GCAGACGTCT GTCCATGGGA CTTTGAGGAA GTATCAAGCA AGCCAAAGGA CTCCCCTCGC 
TCCCCTGATC AGAGCAGACC AAAAAAGGGC ACCACACCAA CTGAGATCAA AGGCAAAAAA 
AGCTCCTCGC CTACTGAAGT CAAGGGCAAG GGTACTACGC CTACAGAGGT CAAAGCAAAG 
AGTATTCTCT CTGAACCTAC TAAATCCACA GGCAGCTCAC TGCAGCCGCC CTCTAAAGCA 
GACGTGTGTC CATGGGACTT TGAGACTATG TCAAGTCCCA CCTCTGAAAA GACCCCTTGT 
TCTTCTCAGG TTCCTAAATC AAAGGAGACC CATGCTATAA AGAAGGGAAA TTCCCCTGTC 
AGATTATTAG AGTTGGAAAG AGGAAACACA AAGGGTCCAG ATGAGGACAA AATGAAATCA 
AACACAAAGT TCAGCTCTGG CAAGACAAAA GAAAGACCCG TGAGCCAGTC TAAAATAATG 
GCCGAGGTTT GTCCGTGGGA TGTTGATAGT AGTCAGGGAA ATGTTTCAAT GGAAAAACAA 
AAAAGCCCAA ATGTTGCATT AGCAAAAAGC AAACAAGCAG ACGTATGTCC TTGGGATTTT 
GAAGAGGCAG CAACTAGTAA GGAGGCCAAC AGGGGCACCG GTGGCAAACA GAAAAGTTCA 
AATTCTAGTG GTGTTAAAAC GGCAGATGTC TGTCCATGGG ACTTTGATGA TCAAGCATCG 
ACAAAAAAGG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gpr158a ( XM_005162534.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162591.1 Danio rerio G protein-coupled receptor 158a (gpr158a), transcript variant X1, mRNA.

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CloneID	ODa28308
Clone ID Related Accession (Same CDS sequence)	XM_005162534.3 , XM_005162534.4
Accession Version	XM_005162534.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4095bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	probable G-protein coupled receptor 158 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162534.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATAA TCCGGATATC GTTACTCTTG CAAGTGGGAT TTGTTATAGG ATCAAATTAT 
AGATATGTGG AACATGACCG GGACCTGAAA GGAAAGCTGC CCATTTACAA CACGCACAGC 
AACCAGCACG CGCACCTCCG GAAGCCCCAT TCGGGTTTAG TGCAGAAGAT CGAGGAGCAG 
GTACCCAAAG TAGTGACTGC GTTTCTGCAC ACGGGGGATT CCTCCACCTT AAAGCACGCC 
AACTGCTCCA GGAGATATGA GCTGGCGGTG TTGCGGGGAA GGACGCGCGG AGACGCGCAT 
CACTCCATGC GCAGCGTCCT GGACACGGTC CTTCACGCCA CAAACTTCCT CAACATGATA 
TTACAAACAA ACCGATCCAG AAATCACAGC CCCAGACATG CAGACATGGA GTGGTACAAC 
GCGCTGGTCA GGAGTATCCT GGAGGGAGAC CTCAAGATCC ACCGAGCGGT GGTCACCTTG 
AGCACAGAAT CCCCTCCTGA AGGACCACCT GTTTACCTTC AGGCTACACG GGCAGGTGGA 
GAGATCATTC TGCAGGATTT ATCCAACATT ACCCATCGGA TGTTGAGAAA CAGAACGGCT 
GACACCGAGT GGTATCATGA GCACAAACAT AAAAAGAAGA CACATCTGCA AAAACGAGTG 
CTGAGTCAAG ATTTTGCCTC GTTTGACGCG TCCGTGCGCA GTGGAGAAAG TTATATTACC 
GAAAAAACTC AGATACACTG GTCAGCTCCG TACTTGGAGT GCGAGAATGG GAATTTCATA 
CCCAGATGGC TCTTGACACT GTCAGCTGGC TTCTATGGCC TTAAACCCAA CAGGAACCCA 
GACTTCAGGG GTGTTGTTCG AGTGGACGTC AGCCTGCAGG ATGTGGATAT TGACCAGTGC 
TCTGCAGACG GCTGGTTTGC AGGAACACAC AGGTGTAACA TCACCACTAT GGAGTGTTTG 
CCGGTTCCTG GGCATGGATT TGTGCTGGAC AAGTATAAAT GTCACTGCAA AAGTGGCTAT 
TATCATCCAA GCAGAGTTGC AGTGAATGGC TTTAAAAGGA AAGCAGGAGG GAAGGGGACC 
GAGGGTTTGT CCCATAACAG GGATGTTCCA GAAACTTCCA GCCACTGTCT GCCCTGCCAG 
GAAGGCTGTT CTTTCTGCAA GGACGATACG CCCTGCGTGG CCCGGGGTGA TGGAGCCCTG 
CGTATGGCGG TGCTCTCCTT CCAGGGCCTG TGCATGCTGG TGGATTTCAT CAGTATGGTG 
CTGCTTTACC ATTTCCGCAG GAACAAGAGT ATCAGAGCAT CAGGACTCAT TCTGCTGGAG 
GCCATCTTGT TTGGAGCTCT TCTCCTGTAC TTTCCGGTGG TGATCCTGTA CTTCCAGCCA 
AGTGTTTTCC GCTGTATCCT GCTGCGATGG GTCCGTCTGC TCGGGTTTGC CACTGTATAC 
GGAACAGTCA CTCTCAAGCT CTACAGGGTC CTCAAGGTGT TCCTGTCACG TACAGCCCAG 
CGGATCCCTT ACATGACCAG CTGGAGAGTG TTGCGTCTAC TGTGTATCAT CCTGTTGATC 
GTGTTATGGT TCGCAATAGC CTGGACCTCC GCTGTTTGTC AGAATCCAAA CAGGCACCTC 
GCTCTCATCA GTGTGGGCTT CACCACTGAC GGACTGCAGT TCAGCATGTG TCTGCTGGAC 
CGCTGGGACT ACATGATTGC TGTGGCTGAG TTCCTGTTTC TTCTTTGGGG TGTATATCTT 
TGCTTTGCGG TGAGGACCGT CCCATCGGCA TATCACGAAC CACGCTACAT GGCCATCGCA 
GTTCACAATG AGCTCATCCT CTCGGCCATA TTCCACATTT TAAGGTTCAC ACTTGCTCCT 
GAATTGCACC CAGACTGGAT GCTGATGCTT TTCTTTGCTC ATACACACTT AACGGTGACT 
GTCACTTTGG GGTTGCTGCT TGTGCCAAAG TTCTTGTTTG CGGGCACACA TCTAAGGGAT 
GACATTGCTA CAGAAGCTTA CGAAGATGAA TTGGACATGG GGCGGTCTGG GTCATACCTG 
AATAGCAGTA TCACATCTGC TTGGAGTGAG CACAGTCTGG ACCCAGAGGA TATCCGGACA 
CCAGAGGAAA TGGGCCAGCT AAGTTCTATT AATGGCTGTG GCGTAAGGTC TTGCGACAGT 
CTCTGGGAAA AACGTACCAA CGAAGAGCTG AAGAAACTCT ACTCTCAACT GGAGATTTAC 
AAGAGGAAAA AGATGCTGGC TAACAATCCT CACCTGCAGA AGAAGCGAAG CTCCAAAAAG 
GGCCTAGGCC GCTCTCTCAT GCGACGCATC ACTGAGATCC CAGAGACAAT GGGACGCCAG 
TGTAGCCGGG ACGACAAAGA CCAAGGTGAA CACGGGAGCA ACCATAACAG CATCTGTGTG 
CTGAGAAAGA ATCCTTTTGA TCCCTCACAC TCTGTTAAGC CTGCTAAAGA TGAATCCTTA 
AAGAGCAAAG TTTTCTCCCT TAAAAAGTCC CACAGCAGTT ACGACCATGT TCGAGACCAC 
AGTGAAGATT CCAGCAGTTC GGCAACTGAC AAGATGGAAG TCACAACCAC AGAGGGCTCT 
CTTCTCGAAA CCCTAATGGG AAAAAAGCTG GTCAAAAAGT CTTCTGAAAA GATTGATGTA 
GCCAGTGAGT CAACAGAGTC GGTTCCCTTG GTGTGCAAGT CAGCCAGCGC TCACAATCTG 
ACAGCTGATA AGAAACCTAT TCACCCCAGA ACATCCATGC TGCAGAAATC TCTAAGCGTT 
ATTGCCAGTG CCAAAGAGAA AACTCTTGGT CTAACAGGAA AGGCCCAGGG TGTAGAGGAT 
TCAACAAAGA AAGCCAATCA AAAGAACAGT GAGGGCAAGA CCTCAGCTGA AGCTGAAGAA 
AAAGAAGGTT ATCCGAAGAT GATTGTCAGT CAATCAGTGG AATACAAGCA GACAGCAGCT 
AAAACGGGTA TAATGAAGCC ACAAATGAGC GGAAGCCAGC CCTCCATTTG CTCAGAAACA 
GCCAAGGGGA AAGACCTGTG TGACCTCTCA GAAGTGTGCC CTTGGGAAGT GGAGGATTTG 
CCAACTCCCT CCGAAAATAA AGTCCAGAAA CATGTGTCTA TTGCTCCTGA GCAAACAAAC 
ACAGTTCGTG GAGGTAGCAG CAAGACGACA AAGTCACAAC AGCAAAAGCA AAAAGGAGCA 
GAGCAGTCAC CTTCTGCGGC CAGGCATCCA AACAAGAAAG CTGCAGATAG AGCAGACATA 
TGTCCGTGGG AGGAAGGAAA CGAGGATCAT GGTCAGCCAA CAGGGCCAAA ACCTCACTCC 
AATACTAAGT CTGCCATGTC TCAACAACAG GCTTCCAGAG AGGTTGCCAA GGTTTTAAAA 
GCAGACGTCT GTCCATGGGA CTTTGAGGAA GTATCAAGCA AGCCAAAGGA CTCCCCTCGC 
TCCCCTGATC AGAGCAGACC AAAAAAGGGC ACCACACCAA CTGAGATCAA AGGCAAAAAA 
AGCTCCTCGC CTACTGAAGT CAAGGGCAAG GGTACTACGC CTACAGAGGT CAAAGCAAAG 
AGTATTCTCT CTGAACCTAC TAAATCCACA GGCAGCTCAC TGCAGCCGCC CTCTAAAGCA 
GACGTGTGTC CATGGGACTT TGAGACTATG TCAAGTCCCA CCTCTGAAAA GACCCCTTGT 
TCTTCTCAGG TTCCTAAATC AAAGGAGACC CATGCTATAA AGAAGGGAAA TTCCCCTGTC 
AGATTATTAG AGTTGGAAAG AGGAAACACA AAGGGTCCAG ATGAGGACAA AATGAAATCA 
AACACAAAGT TCAGCTCTGG CAAGACAAAA GAAAGACCCG TGAGCCAGTC TAAAATAATG 
GCCGAGGTTT GTCCGTGGGA TGTTGATAGT AGTCAGGGAA ATGTTTCAAT GGAAAAACAA 
AAAAGCCCAA ATGTTGCATT AGCAAAAAGC AAACAAGCAG ACGTATGTCC TTGGGATTTT 
GAAGAGGCAG CAACTAGTAA GGAGGCCAAC AGGGGCACCG GTGGCAAACA GAAAAGTTCA 
AATTCTAGTG GTGTTAAAAC GGCAGATGTC TGTCCATGGG ACTTTGATGA TCAAGCATCG 
ACAAAAAAGG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162591.1
CDS	1987..6081
Translation	MAIIRISLLLQVGFVIGSNYRYVEHDRDLKGKLPIYNTHSNQHAHLRKPHSGLVQKIEEQVPKVVTAFLHTGDSSTLKHANCSRRYELAVLRGRTRGDAHHSMRSVLDTVLHATNFLNMILQTNRSRNHSPRHADMEWYNALVRSILEGDLKIHRAVVTLSTESPPEGPPVYLQATRAGGEIILQDLSNITHRMLRNRTADTEWYHEHKHKKKTHLQKRVLSQDFASFDASVRSGESYITEKTQIHWSAPYLECENGNFIPRWLLTLSAGFYGLKPNRNPDFRGVVRVDVSLQDVDIDQCSADGWFAGTHRCNITTMECLPVPGHGFVLDKYKCHCKSGYYHPSRVAVNGFKRKAGGKGTEGLSHNRDVPETSSHCLPCQEGCSFCKDDTPCVARGDGALRMAVLSFQGLCMLVDFISMVLLYHFRRNKSIRASGLILLEAILFGALLLYFPVVILYFQPSVFRCILLRWVRLLGFATVYGTVTLKLYRVLKVFLSRTAQRIPYMTSWRVLRLLCIILLIVLWFAIAWTSAVCQNPNRHLALISVGFTTDGLQFSMCLLDRWDYMIAVAEFLFLLWGVYLCFAVRTVPSAYHEPRYMAIAVHNELILSAIFHILRFTLAPELHPDWMLMLFFAHTHLTVTVTLGLLLVPKFLFAGTHLRDDIATEAYEDELDMGRSGSYLNSSITSAWSEHSLDPEDIRTPEEMGQLSSINGCGVRSCDSLWEKRTNEELKKLYSQLEIYKRKKMLANNPHLQKKRSSKKGLGRSLMRRITEIPETMGRQCSRDDKDQGEHGSNHNSICVLRKNPFDPSHSVKPAKDESLKSKVFSLKKSHSSYDHVRDHSEDSSSSATDKMEVTTTEGSLLETLMGKKLVKKSSEKIDVASESTESVPLVCKSASAHNLTADKKPIHPRTSMLQKSLSVIASAKEKTLGLTGKAQGVEDSTKKANQKNSEGKTSAEAEEKEGYPKMIVSQSVEYKQTAAKTGIMKPQMSGSQPSICSETAKGKDLCDLSEVCPWEVEDLPTPSENKVQKHVSIAPEQTNTVRGGSSKTTKSQQQKQKGAEQSPSAARHPNKKAADRADICPWEEGNEDHGQPTGPKPHSNTKSAMSQQQASREVAKVLKADVCPWDFEEVSSKPKDSPRSPDQSRPKKGTTPTEIKGKKSSSPTEVKGKGTTPTEVKAKSILSEPTKSTGSSLQPPSKADVCPWDFETMSSPTSEKTPCSSQVPKSKETHAIKKGNSPVRLLELERGNTKGPDEDKMKSNTKFSSGKTKERPVSQSKIMAEVCPWDVDSSQGNVSMEKQKSPNVALAKSKQADVCPWDFEEAATSKEANRGTGGKQKSSNSSGVKTADVCPWDFDDQASTKKA

Target ORF information:

RefSeq Version	XM_005162534.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio G protein-coupled receptor 158a (gpr158a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162534.4

ORF Insert Sequence:

ATGGCCATAA TCCGGATATC GTTACTCTTG CAAGTGGGAT TTGTTATAGG ATCAAATTAT 
AGATATGTGG AACATGACCG GGACCTGAAA GGAAAGCTGC CCATTTACAA CACGCACAGC 
AACCAGCACG CGCACCTCCG GAAGCCCCAT TCGGGTTTAG TGCAGAAGAT CGAGGAGCAG 
GTACCCAAAG TAGTGACTGC GTTTCTGCAC ACGGGGGATT CCTCCACCTT AAAGCACGCC 
AACTGCTCCA GGAGATATGA GCTGGCGGTG TTGCGGGGAA GGACGCGCGG AGACGCGCAT 
CACTCCATGC GCAGCGTCCT GGACACGGTC CTTCACGCCA CAAACTTCCT CAACATGATA 
TTACAAACAA ACCGATCCAG AAATCACAGC CCCAGACATG CAGACATGGA GTGGTACAAC 
GCGCTGGTCA GGAGTATCCT GGAGGGAGAC CTCAAGATCC ACCGAGCGGT GGTCACCTTG 
AGCACAGAAT CCCCTCCTGA AGGACCACCT GTTTACCTTC AGGCTACACG GGCAGGTGGA 
GAGATCATTC TGCAGGATTT ATCCAACATT ACCCATCGGA TGTTGAGAAA CAGAACGGCT 
GACACCGAGT GGTATCATGA GCACAAACAT AAAAAGAAGA CACATCTGCA AAAACGAGTG 
CTGAGTCAAG ATTTTGCCTC GTTTGACGCG TCCGTGCGCA GTGGAGAAAG TTATATTACC 
GAAAAAACTC AGATACACTG GTCAGCTCCG TACTTGGAGT GCGAGAATGG GAATTTCATA 
CCCAGATGGC TCTTGACACT GTCAGCTGGC TTCTATGGCC TTAAACCCAA CAGGAACCCA 
GACTTCAGGG GTGTTGTTCG AGTGGACGTC AGCCTGCAGG ATGTGGATAT TGACCAGTGC 
TCTGCAGACG GCTGGTTTGC AGGAACACAC AGGTGTAACA TCACCACTAT GGAGTGTTTG 
CCGGTTCCTG GGCATGGATT TGTGCTGGAC AAGTATAAAT GTCACTGCAA AAGTGGCTAT 
TATCATCCAA GCAGAGTTGC AGTGAATGGC TTTAAAAGGA AAGCAGGAGG GAAGGGGACC 
GAGGGTTTGT CCCATAACAG GGATGTTCCA GAAACTTCCA GCCACTGTCT GCCCTGCCAG 
GAAGGCTGTT CTTTCTGCAA GGACGATACG CCCTGCGTGG CCCGGGGTGA TGGAGCCCTG 
CGTATGGCGG TGCTCTCCTT CCAGGGCCTG TGCATGCTGG TGGATTTCAT CAGTATGGTG 
CTGCTTTACC ATTTCCGCAG GAACAAGAGT ATCAGAGCAT CAGGACTCAT TCTGCTGGAG 
GCCATCTTGT TTGGAGCTCT TCTCCTGTAC TTTCCGGTGG TGATCCTGTA CTTCCAGCCA 
AGTGTTTTCC GCTGTATCCT GCTGCGATGG GTCCGTCTGC TCGGGTTTGC CACTGTATAC 
GGAACAGTCA CTCTCAAGCT CTACAGGGTC CTCAAGGTGT TCCTGTCACG TACAGCCCAG 
CGGATCCCTT ACATGACCAG CTGGAGAGTG TTGCGTCTAC TGTGTATCAT CCTGTTGATC 
GTGTTATGGT TCGCAATAGC CTGGACCTCC GCTGTTTGTC AGAATCCAAA CAGGCACCTC 
GCTCTCATCA GTGTGGGCTT CACCACTGAC GGACTGCAGT TCAGCATGTG TCTGCTGGAC 
CGCTGGGACT ACATGATTGC TGTGGCTGAG TTCCTGTTTC TTCTTTGGGG TGTATATCTT 
TGCTTTGCGG TGAGGACCGT CCCATCGGCA TATCACGAAC CACGCTACAT GGCCATCGCA 
GTTCACAATG AGCTCATCCT CTCGGCCATA TTCCACATTT TAAGGTTCAC ACTTGCTCCT 
GAATTGCACC CAGACTGGAT GCTGATGCTT TTCTTTGCTC ATACACACTT AACGGTGACT 
GTCACTTTGG GGTTGCTGCT TGTGCCAAAG TTCTTGTTTG CGGGCACACA TCTAAGGGAT 
GACATTGCTA CAGAAGCTTA CGAAGATGAA TTGGACATGG GGCGGTCTGG GTCATACCTG 
AATAGCAGTA TCACATCTGC TTGGAGTGAG CACAGTCTGG ACCCAGAGGA TATCCGGACA 
CCAGAGGAAA TGGGCCAGCT AAGTTCTATT AATGGCTGTG GCGTAAGGTC TTGCGACAGT 
CTCTGGGAAA AACGTACCAA CGAAGAGCTG AAGAAACTCT ACTCTCAACT GGAGATTTAC 
AAGAGGAAAA AGATGCTGGC TAACAATCCT CACCTGCAGA AGAAGCGAAG CTCCAAAAAG 
GGCCTAGGCC GCTCTCTCAT GCGACGCATC ACTGAGATCC CAGAGACAAT GGGACGCCAG 
TGTAGCCGGG ACGACAAAGA CCAAGGTGAA CACGGGAGCA ACCATAACAG CATCTGTGTG 
CTGAGAAAGA ATCCTTTTGA TCCCTCACAC TCTGTTAAGC CTGCTAAAGA TGAATCCTTA 
AAGAGCAAAG TTTTCTCCCT TAAAAAGTCC CACAGCAGTT ACGACCATGT TCGAGACCAC 
AGTGAAGATT CCAGCAGTTC GGCAACTGAC AAGATGGAAG TCACAACCAC AGAGGGCTCT 
CTTCTCGAAA CCCTAATGGG AAAAAAGCTG GTCAAAAAGT CTTCTGAAAA GATTGATGTA 
GCCAGTGAGT CAACAGAGTC GGTTCCCTTG GTGTGCAAGT CAGCCAGCGC TCACAATCTG 
ACAGCTGATA AGAAACCTAT TCACCCCAGA ACATCCATGC TGCAGAAATC TCTAAGCGTT 
ATTGCCAGTG CCAAAGAGAA AACTCTTGGT CTAACAGGAA AGGCCCAGGG TGTAGAGGAT 
TCAACAAAGA AAGCCAATCA AAAGAACAGT GAGGGCAAGA CCTCAGCTGA AGCTGAAGAA 
AAAGAAGGTT ATCCGAAGAT GATTGTCAGT CAATCAGTGG AATACAAGCA GACAGCAGCT 
AAAACGGGTA TAATGAAGCC ACAAATGAGC GGAAGCCAGC CCTCCATTTG CTCAGAAACA 
GCCAAGGGGA AAGACCTGTG TGACCTCTCA GAAGTGTGCC CTTGGGAAGT GGAGGATTTG 
CCAACTCCCT CCGAAAATAA AGTCCAGAAA CATGTGTCTA TTGCTCCTGA GCAAACAAAC 
ACAGTTCGTG GAGGTAGCAG CAAGACGACA AAGTCACAAC AGCAAAAGCA AAAAGGAGCA 
GAGCAGTCAC CTTCTGCGGC CAGGCATCCA AACAAGAAAG CTGCAGATAG AGCAGACATA 
TGTCCGTGGG AGGAAGGAAA CGAGGATCAT GGTCAGCCAA CAGGGCCAAA ACCTCACTCC 
AATACTAAGT CTGCCATGTC TCAACAACAG GCTTCCAGAG AGGTTGCCAA GGTTTTAAAA 
GCAGACGTCT GTCCATGGGA CTTTGAGGAA GTATCAAGCA AGCCAAAGGA CTCCCCTCGC 
TCCCCTGATC AGAGCAGACC AAAAAAGGGC ACCACACCAA CTGAGATCAA AGGCAAAAAA 
AGCTCCTCGC CTACTGAAGT CAAGGGCAAG GGTACTACGC CTACAGAGGT CAAAGCAAAG 
AGTATTCTCT CTGAACCTAC TAAATCCACA GGCAGCTCAC TGCAGCCGCC CTCTAAAGCA 
GACGTGTGTC CATGGGACTT TGAGACTATG TCAAGTCCCA CCTCTGAAAA GACCCCTTGT 
TCTTCTCAGG TTCCTAAATC AAAGGAGACC CATGCTATAA AGAAGGGAAA TTCCCCTGTC 
AGATTATTAG AGTTGGAAAG AGGAAACACA AAGGGTCCAG ATGAGGACAA AATGAAATCA 
AACACAAAGT TCAGCTCTGG CAAGACAAAA GAAAGACCCG TGAGCCAGTC TAAAATAATG 
GCCGAGGTTT GTCCGTGGGA TGTTGATAGT AGTCAGGGAA ATGTTTCAAT GGAAAAACAA 
AAAAGCCCAA ATGTTGCATT AGCAAAAAGC AAACAAGCAG ACGTATGTCC TTGGGATTTT 
GAAGAGGCAG CAACTAGTAA GGAGGCCAAC AGGGGCACCG GTGGCAAACA GAAAAGTTCA 
AATTCTAGTG GTGTTAAAAC GGCAGATGTC TGTCCATGGG ACTTTGATGA TCAAGCATCG 
ACAAAAAAGG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gpr158a ( XM_005162535.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162592.1 Danio rerio G protein-coupled receptor 158a (gpr158a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa28309
Clone ID Related Accession (Same CDS sequence)	XM_005162535.4 , XM_005162535.3
Accession Version	XM_005162535.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	probable G-protein coupled receptor 158 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162535.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATAA TCCGGATATC GTTACTCTTG CAAGTGGGAT TTGTTATAGG ATCAAATTAT 
AGATATGTGG AACATGACCG GGACCTGAAA GGAAAGCTGC CCATTTACAA CACGCACAGC 
AACCAGCACG CGCACCTCCG GAAGCCCCAT TCGGGTTTAG TGCAGAAGAT CGAGGAGCAG 
GTACCCAAAG TAGTGACTGC GTTTCTGCAC ACGGGGGATT CCTCCACCTT AAAGCACGCC 
AACTGCTCCA GGAGATATGA GCTGGCGGTG TTGCGGGGAA GGACGCGCGG AGACGCGCAT 
CACTCCATGC GCAGCGTCCT GGACACGGTC CTTCACGCCA CAAACTTCCT CAACATGATA 
TTACAAACAA ACCGATCCAG AAATCACAGC CCCAGACATG CAGACATGGA GTGGTACAAC 
GCGCTGGTCA GGAGTATCCT GGAGGGAGAC CTCAAGATCC ACCGAGCGGT GGTCACCTTG 
AGCACAGAAT CCCCTCCTGA AGGACCACCT GTTTACCTTC AGGCTACACG GGCAGGTGGA 
GAGATCATTC TGCAGGATTT ATCCAACATT ACCCATCGGA TGTTGAGAAA CAGAACGGCT 
GACACCGAGT GGTATCATGA GCACAAACAT AAAAAGAAGA CACATCTGCA AAAACGAGTG 
CTGAGTCAAG ATTTTGCCTC GTTTGACGCG TCCGTGCGCA GTGGAGAAAG TTATATTACC 
GAAAAAACTC AGATACACTG GTCAGCTCCG TACTTGGAGT GCGAGAATGG GAATTTCATA 
CCCAGATGGC TCTTGACACT GTCAGCTGGC TTCTATGGCC TTAAACCCAA CAGGAACCCA 
GACTTCAGGG GTGTTGTTCG AGTGGACGTC AGCCTGCAGG ATGTGGATAT TGACCAGTGC 
TCTGCAGACG GCTGGTTTGC AGGAACACAC AGGTGTAACA TCACCACTAT GGAGTGTTTG 
CCGGTTCCTG GGCATGGATT TGTGCTGGAC AAGTATAAAT GTCACTGCAA AAGTGGCTAT 
TATCATCCAA GCAGAGTTGC AGTGAATGGC TTTAAAAGGA AAGCAGGAGG GAAGGGGACC 
GAGGGTTTGT CCCATAACAG GGATGTTCCA GAAACTTCCA GCCACTGTCT GCCCTGCCAG 
GAAGGCTGTT CTTTCTGCAA GGACGATACG CCCTGCGTGG CCCGGGGTGA TGGAGCCCTG 
CGTATGGCGG TGCTCTCCTT CCAGGGCCTG TGCATGCTGG TGGATTTCAT CAGTATGGTG 
CTGCTTTACC ATTTCCGCAG GAACAAGAGT ATCAGAGCAT CAGGACTCAT TCTGCTGGAG 
GCCATCTTGT TTGGAGCTCT TCTCCTGTAC TTTCCGGTGG TGATCCTGTA CTTCCAGCCA 
AGTGTTTTCC GCTGTATCCT GCTGCGATGG GTCCGTCTGC TCGGGTTTGC CACTGTATAC 
GGAACAGTCA CTCTCAAGCT CTACAGGGTC CTCAAGGTGT TCCTGTCACG TACAGCCCAG 
CGGATCCCTT ACATGACCAG CTGGAGAGTG TTGCGTCTAC TGTGTATCAT CCTGTTGATC 
GTGTTATGGT TCGCAATAGC CTGGACCTCC GCTGTTTGTC AGAATCCAAA CAGGCACCTC 
GCTCTCATCA GTGTGGGCTT CACCACTGAC GGACTGCAGT TCAGCATGTG TCTGCTGGAC 
CGCTGGGACT ACATGATTGC TGTGGCTGAG TTCCTGTTTC TTCTTTGGGG TGTATATCTT 
TGCTTTGCGG TGAGGACCGT CCCATCGGCA TATCACGAAC CACGCTACAT GGCCATCGCA 
GTTCACAATG AGCTCATCCT CTCGGCCATA TTCCACATTT TAAGGTTCAC ACTTGCTCCT 
GAATTGCACC CAGACTGGAT GCTGATGCTT TTCTTTGCTC ATACACACTT AACGGTGACT 
GTCACTTTGG GGTTGCTGCT TGTGCCAAAG TTCTTGTTTG CGGGCACACA TCTAAGGGAT 
GACATTGCTA CAGAAGCTTA CGAAGATGAA TTGGACATGG GGCGGTCTGG GTCATACCTG 
AATAGCAGTA TCACATCTGC TTGGAGTGAG CACAGTCTGG ACCCAGAGGA TATCCGGGAA 
GAGCTGAAGA AACTCTACTC TCAACTGGAG ATTTACAAGA GGAAAAAGAT GCTGGCTAAC 
AATCCTCACC TGCAGAAGAA GCGAAGCTCC AAAAAGGGCC TAGGCCGCTC TCTCATGCGA 
CGCATCACTG AGATCCCAGA GACAATGGGA CGCCAGTGTA GCCGGGACGA CAAAGACCAA 
GGTGAACACG GGAGCAACCA TAACAGCATC TGTGTGCTGA GAAAGAATCC TTTTGATCCC 
TCACACTCTG TTAAGCCTGC TAAAGATGAA TCCTTAAAGA GCAAAGTTTT CTCCCTTAAA 
AAGTCCCACA GCAGTTACGA CCATGTTCGA GACCACAGTG AAGATTCCAG CAGTTCGGCA 
ACTGACAAGA TGGAAGTCAC AACCACAGAG GGCTCTCTTC TCGAAACCCT AATGGGAAAA 
AAGCTGGTCA AAAAGTCTTC TGAAAAGATT GATGTAGCCA GTGAGTCAAC AGAGTCGGTT 
CCCTTGGTGT GCAAGTCAGC CAGCGCTCAC AATCTGACAG CTGATAAGAA ACCTATTCAC 
CCCAGAACAT CCATGCTGCA GAAATCTCTA AGCGTTATTG CCAGTGCCAA AGAGAAAACT 
CTTGGTCTAA CAGGAAAGGC CCAGGGTGTA GAGGATTCAA CAAAGAAAGC CAATCAAAAG 
AACAGTGAGG GCAAGACCTC AGCTGAAGCT GAAGAAAAAG AAGGTTATCC GAAGATGATT 
GTCAGTCAAT CAGTGGAATA CAAGCAGACA GCAGCTAAAA CGGGTATAAT GAAGCCACAA 
ATGAGCGGAA GCCAGCCCTC CATTTGCTCA GAAACAGCCA AGGGGAAAGA CCTGTGTGAC 
CTCTCAGAAG TGTGCCCTTG GGAAGTGGAG GATTTGCCAA CTCCCTCCGA AAATAAAGTC 
CAGAAACATG TGTCTATTGC TCCTGAGCAA ACAAACACAG TTCGTGGAGG TAGCAGCAAG 
ACGACAAAGT CACAACAGCA AAAGCAAAAA GGAGCAGAGC AGTCACCTTC TGCGGCCAGG 
CATCCAAACA AGAAAGCTGC AGATAGAGCA GACATATGTC CGTGGGAGGA AGGAAACGAG 
GATCATGGTC AGCCAACAGG GCCAAAACCT CACTCCAATA CTAAGTCTGC CATGTCTCAA 
CAACAGGCTT CCAGAGAGGT TGCCAAGGTT TTAAAAGCAG ACGTCTGTCC ATGGGACTTT 
GAGGAAGTAT CAAGCAAGCC AAAGGACTCC CCTCGCTCCC CTGATCAGAG CAGACCAAAA 
AAGGGCACCA CACCAACTGA GATCAAAGGC AAAAAAAGCT CCTCGCCTAC TGAAGTCAAG 
GGCAAGGGTA CTACGCCTAC AGAGGTCAAA GCAAAGAGTA TTCTCTCTGA ACCTACTAAA 
TCCACAGGCA GCTCACTGCA GCCGCCCTCT AAAGCAGACG TGTGTCCATG GGACTTTGAG 
ACTATGTCAA GTCCCACCTC TGAAAAGACC CCTTGTTCTT CTCAGGTTCC TAAATCAAAG 
GAGACCCATG CTATAAAGAA GGGAAATTCC CCTGTCAGAT TATTAGAGTT GGAAAGAGGA 
AACACAAAGG GTCCAGATGA GGACAAAATG AAATCAAACA CAAAGTTCAG CTCTGGCAAG 
ACAAAAGAAA GACCCGTGAG CCAGTCTAAA ATAATGGCCG AGGTTTGTCC GTGGGATGTT 
GATAGTAGTC AGGGAAATGT TTCAATGGAA AAACAAAAAA GCCCAAATGT TGCATTAGCA 
AAAAGCAAAC AAGCAGACGT ATGTCCTTGG GATTTTGAAG AGGCAGCAAC TAGTAAGGAG 
GCCAACAGGG GCACCGGTGG CAAACAGAAA AGTTCAAATT CTAGTGGTGT TAAAACGGCA 
GATGTCTGTC CATGGGACTT TGATGATCAA GCATCGACAA AAAAGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162592.1
CDS	1986..5996
Translation	MAIIRISLLLQVGFVIGSNYRYVEHDRDLKGKLPIYNTHSNQHAHLRKPHSGLVQKIEEQVPKVVTAFLHTGDSSTLKHANCSRRYELAVLRGRTRGDAHHSMRSVLDTVLHATNFLNMILQTNRSRNHSPRHADMEWYNALVRSILEGDLKIHRAVVTLSTESPPEGPPVYLQATRAGGEIILQDLSNITHRMLRNRTADTEWYHEHKHKKKTHLQKRVLSQDFASFDASVRSGESYITEKTQIHWSAPYLECENGNFIPRWLLTLSAGFYGLKPNRNPDFRGVVRVDVSLQDVDIDQCSADGWFAGTHRCNITTMECLPVPGHGFVLDKYKCHCKSGYYHPSRVAVNGFKRKAGGKGTEGLSHNRDVPETSSHCLPCQEGCSFCKDDTPCVARGDGALRMAVLSFQGLCMLVDFISMVLLYHFRRNKSIRASGLILLEAILFGALLLYFPVVILYFQPSVFRCILLRWVRLLGFATVYGTVTLKLYRVLKVFLSRTAQRIPYMTSWRVLRLLCIILLIVLWFAIAWTSAVCQNPNRHLALISVGFTTDGLQFSMCLLDRWDYMIAVAEFLFLLWGVYLCFAVRTVPSAYHEPRYMAIAVHNELILSAIFHILRFTLAPELHPDWMLMLFFAHTHLTVTVTLGLLLVPKFLFAGTHLRDDIATEAYEDELDMGRSGSYLNSSITSAWSEHSLDPEDIREELKKLYSQLEIYKRKKMLANNPHLQKKRSSKKGLGRSLMRRITEIPETMGRQCSRDDKDQGEHGSNHNSICVLRKNPFDPSHSVKPAKDESLKSKVFSLKKSHSSYDHVRDHSEDSSSSATDKMEVTTTEGSLLETLMGKKLVKKSSEKIDVASESTESVPLVCKSASAHNLTADKKPIHPRTSMLQKSLSVIASAKEKTLGLTGKAQGVEDSTKKANQKNSEGKTSAEAEEKEGYPKMIVSQSVEYKQTAAKTGIMKPQMSGSQPSICSETAKGKDLCDLSEVCPWEVEDLPTPSENKVQKHVSIAPEQTNTVRGGSSKTTKSQQQKQKGAEQSPSAARHPNKKAADRADICPWEEGNEDHGQPTGPKPHSNTKSAMSQQQASREVAKVLKADVCPWDFEEVSSKPKDSPRSPDQSRPKKGTTPTEIKGKKSSSPTEVKGKGTTPTEVKAKSILSEPTKSTGSSLQPPSKADVCPWDFETMSSPTSEKTPCSSQVPKSKETHAIKKGNSPVRLLELERGNTKGPDEDKMKSNTKFSSGKTKERPVSQSKIMAEVCPWDVDSSQGNVSMEKQKSPNVALAKSKQADVCPWDFEEAATSKEANRGTGGKQKSSNSSGVKTADVCPWDFDDQASTKKA

Target ORF information:

RefSeq Version	XM_005162535.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio G protein-coupled receptor 158a (gpr158a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162535.4

ORF Insert Sequence:

ATGGCCATAA TCCGGATATC GTTACTCTTG CAAGTGGGAT TTGTTATAGG ATCAAATTAT 
AGATATGTGG AACATGACCG GGACCTGAAA GGAAAGCTGC CCATTTACAA CACGCACAGC 
AACCAGCACG CGCACCTCCG GAAGCCCCAT TCGGGTTTAG TGCAGAAGAT CGAGGAGCAG 
GTACCCAAAG TAGTGACTGC GTTTCTGCAC ACGGGGGATT CCTCCACCTT AAAGCACGCC 
AACTGCTCCA GGAGATATGA GCTGGCGGTG TTGCGGGGAA GGACGCGCGG AGACGCGCAT 
CACTCCATGC GCAGCGTCCT GGACACGGTC CTTCACGCCA CAAACTTCCT CAACATGATA 
TTACAAACAA ACCGATCCAG AAATCACAGC CCCAGACATG CAGACATGGA GTGGTACAAC 
GCGCTGGTCA GGAGTATCCT GGAGGGAGAC CTCAAGATCC ACCGAGCGGT GGTCACCTTG 
AGCACAGAAT CCCCTCCTGA AGGACCACCT GTTTACCTTC AGGCTACACG GGCAGGTGGA 
GAGATCATTC TGCAGGATTT ATCCAACATT ACCCATCGGA TGTTGAGAAA CAGAACGGCT 
GACACCGAGT GGTATCATGA GCACAAACAT AAAAAGAAGA CACATCTGCA AAAACGAGTG 
CTGAGTCAAG ATTTTGCCTC GTTTGACGCG TCCGTGCGCA GTGGAGAAAG TTATATTACC 
GAAAAAACTC AGATACACTG GTCAGCTCCG TACTTGGAGT GCGAGAATGG GAATTTCATA 
CCCAGATGGC TCTTGACACT GTCAGCTGGC TTCTATGGCC TTAAACCCAA CAGGAACCCA 
GACTTCAGGG GTGTTGTTCG AGTGGACGTC AGCCTGCAGG ATGTGGATAT TGACCAGTGC 
TCTGCAGACG GCTGGTTTGC AGGAACACAC AGGTGTAACA TCACCACTAT GGAGTGTTTG 
CCGGTTCCTG GGCATGGATT TGTGCTGGAC AAGTATAAAT GTCACTGCAA AAGTGGCTAT 
TATCATCCAA GCAGAGTTGC AGTGAATGGC TTTAAAAGGA AAGCAGGAGG GAAGGGGACC 
GAGGGTTTGT CCCATAACAG GGATGTTCCA GAAACTTCCA GCCACTGTCT GCCCTGCCAG 
GAAGGCTGTT CTTTCTGCAA GGACGATACG CCCTGCGTGG CCCGGGGTGA TGGAGCCCTG 
CGTATGGCGG TGCTCTCCTT CCAGGGCCTG TGCATGCTGG TGGATTTCAT CAGTATGGTG 
CTGCTTTACC ATTTCCGCAG GAACAAGAGT ATCAGAGCAT CAGGACTCAT TCTGCTGGAG 
GCCATCTTGT TTGGAGCTCT TCTCCTGTAC TTTCCGGTGG TGATCCTGTA CTTCCAGCCA 
AGTGTTTTCC GCTGTATCCT GCTGCGATGG GTCCGTCTGC TCGGGTTTGC CACTGTATAC 
GGAACAGTCA CTCTCAAGCT CTACAGGGTC CTCAAGGTGT TCCTGTCACG TACAGCCCAG 
CGGATCCCTT ACATGACCAG CTGGAGAGTG TTGCGTCTAC TGTGTATCAT CCTGTTGATC 
GTGTTATGGT TCGCAATAGC CTGGACCTCC GCTGTTTGTC AGAATCCAAA CAGGCACCTC 
GCTCTCATCA GTGTGGGCTT CACCACTGAC GGACTGCAGT TCAGCATGTG TCTGCTGGAC 
CGCTGGGACT ACATGATTGC TGTGGCTGAG TTCCTGTTTC TTCTTTGGGG TGTATATCTT 
TGCTTTGCGG TGAGGACCGT CCCATCGGCA TATCACGAAC CACGCTACAT GGCCATCGCA 
GTTCACAATG AGCTCATCCT CTCGGCCATA TTCCACATTT TAAGGTTCAC ACTTGCTCCT 
GAATTGCACC CAGACTGGAT GCTGATGCTT TTCTTTGCTC ATACACACTT AACGGTGACT 
GTCACTTTGG GGTTGCTGCT TGTGCCAAAG TTCTTGTTTG CGGGCACACA TCTAAGGGAT 
GACATTGCTA CAGAAGCTTA CGAAGATGAA TTGGACATGG GGCGGTCTGG GTCATACCTG 
AATAGCAGTA TCACATCTGC TTGGAGTGAG CACAGTCTGG ACCCAGAGGA TATCCGGGAA 
GAGCTGAAGA AACTCTACTC TCAACTGGAG ATTTACAAGA GGAAAAAGAT GCTGGCTAAC 
AATCCTCACC TGCAGAAGAA GCGAAGCTCC AAAAAGGGCC TAGGCCGCTC TCTCATGCGA 
CGCATCACTG AGATCCCAGA GACAATGGGA CGCCAGTGTA GCCGGGACGA CAAAGACCAA 
GGTGAACACG GGAGCAACCA TAACAGCATC TGTGTGCTGA GAAAGAATCC TTTTGATCCC 
TCACACTCTG TTAAGCCTGC TAAAGATGAA TCCTTAAAGA GCAAAGTTTT CTCCCTTAAA 
AAGTCCCACA GCAGTTACGA CCATGTTCGA GACCACAGTG AAGATTCCAG CAGTTCGGCA 
ACTGACAAGA TGGAAGTCAC AACCACAGAG GGCTCTCTTC TCGAAACCCT AATGGGAAAA 
AAGCTGGTCA AAAAGTCTTC TGAAAAGATT GATGTAGCCA GTGAGTCAAC AGAGTCGGTT 
CCCTTGGTGT GCAAGTCAGC CAGCGCTCAC AATCTGACAG CTGATAAGAA ACCTATTCAC 
CCCAGAACAT CCATGCTGCA GAAATCTCTA AGCGTTATTG CCAGTGCCAA AGAGAAAACT 
CTTGGTCTAA CAGGAAAGGC CCAGGGTGTA GAGGATTCAA CAAAGAAAGC CAATCAAAAG 
AACAGTGAGG GCAAGACCTC AGCTGAAGCT GAAGAAAAAG AAGGTTATCC GAAGATGATT 
GTCAGTCAAT CAGTGGAATA CAAGCAGACA GCAGCTAAAA CGGGTATAAT GAAGCCACAA 
ATGAGCGGAA GCCAGCCCTC CATTTGCTCA GAAACAGCCA AGGGGAAAGA CCTGTGTGAC 
CTCTCAGAAG TGTGCCCTTG GGAAGTGGAG GATTTGCCAA CTCCCTCCGA AAATAAAGTC 
CAGAAACATG TGTCTATTGC TCCTGAGCAA ACAAACACAG TTCGTGGAGG TAGCAGCAAG 
ACGACAAAGT CACAACAGCA AAAGCAAAAA GGAGCAGAGC AGTCACCTTC TGCGGCCAGG 
CATCCAAACA AGAAAGCTGC AGATAGAGCA GACATATGTC CGTGGGAGGA AGGAAACGAG 
GATCATGGTC AGCCAACAGG GCCAAAACCT CACTCCAATA CTAAGTCTGC CATGTCTCAA 
CAACAGGCTT CCAGAGAGGT TGCCAAGGTT TTAAAAGCAG ACGTCTGTCC ATGGGACTTT 
GAGGAAGTAT CAAGCAAGCC AAAGGACTCC CCTCGCTCCC CTGATCAGAG CAGACCAAAA 
AAGGGCACCA CACCAACTGA GATCAAAGGC AAAAAAAGCT CCTCGCCTAC TGAAGTCAAG 
GGCAAGGGTA CTACGCCTAC AGAGGTCAAA GCAAAGAGTA TTCTCTCTGA ACCTACTAAA 
TCCACAGGCA GCTCACTGCA GCCGCCCTCT AAAGCAGACG TGTGTCCATG GGACTTTGAG 
ACTATGTCAA GTCCCACCTC TGAAAAGACC CCTTGTTCTT CTCAGGTTCC TAAATCAAAG 
GAGACCCATG CTATAAAGAA GGGAAATTCC CCTGTCAGAT TATTAGAGTT GGAAAGAGGA 
AACACAAAGG GTCCAGATGA GGACAAAATG AAATCAAACA CAAAGTTCAG CTCTGGCAAG 
ACAAAAGAAA GACCCGTGAG CCAGTCTAAA ATAATGGCCG AGGTTTGTCC GTGGGATGTT 
GATAGTAGTC AGGGAAATGT TTCAATGGAA AAACAAAAAA GCCCAAATGT TGCATTAGCA 
AAAAGCAAAC AAGCAGACGT ATGTCCTTGG GATTTTGAAG AGGCAGCAAC TAGTAAGGAG 
GCCAACAGGG GCACCGGTGG CAAACAGAAA AGTTCAAATT CTAGTGGTGT TAAAACGGCA 
GATGTCTGTC CATGGGACTT TGATGATCAA GCATCGACAA AAAAGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gpr158a ( XM_005162535.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162592.1 Danio rerio G protein-coupled receptor 158a (gpr158a), transcript variant X2, mRNA.

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CloneID	ODa28309
Clone ID Related Accession (Same CDS sequence)	XM_005162535.4 , XM_005162535.3
Accession Version	XM_005162535.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4011bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	probable G-protein coupled receptor 158 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162535.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATAA TCCGGATATC GTTACTCTTG CAAGTGGGAT TTGTTATAGG ATCAAATTAT 
AGATATGTGG AACATGACCG GGACCTGAAA GGAAAGCTGC CCATTTACAA CACGCACAGC 
AACCAGCACG CGCACCTCCG GAAGCCCCAT TCGGGTTTAG TGCAGAAGAT CGAGGAGCAG 
GTACCCAAAG TAGTGACTGC GTTTCTGCAC ACGGGGGATT CCTCCACCTT AAAGCACGCC 
AACTGCTCCA GGAGATATGA GCTGGCGGTG TTGCGGGGAA GGACGCGCGG AGACGCGCAT 
CACTCCATGC GCAGCGTCCT GGACACGGTC CTTCACGCCA CAAACTTCCT CAACATGATA 
TTACAAACAA ACCGATCCAG AAATCACAGC CCCAGACATG CAGACATGGA GTGGTACAAC 
GCGCTGGTCA GGAGTATCCT GGAGGGAGAC CTCAAGATCC ACCGAGCGGT GGTCACCTTG 
AGCACAGAAT CCCCTCCTGA AGGACCACCT GTTTACCTTC AGGCTACACG GGCAGGTGGA 
GAGATCATTC TGCAGGATTT ATCCAACATT ACCCATCGGA TGTTGAGAAA CAGAACGGCT 
GACACCGAGT GGTATCATGA GCACAAACAT AAAAAGAAGA CACATCTGCA AAAACGAGTG 
CTGAGTCAAG ATTTTGCCTC GTTTGACGCG TCCGTGCGCA GTGGAGAAAG TTATATTACC 
GAAAAAACTC AGATACACTG GTCAGCTCCG TACTTGGAGT GCGAGAATGG GAATTTCATA 
CCCAGATGGC TCTTGACACT GTCAGCTGGC TTCTATGGCC TTAAACCCAA CAGGAACCCA 
GACTTCAGGG GTGTTGTTCG AGTGGACGTC AGCCTGCAGG ATGTGGATAT TGACCAGTGC 
TCTGCAGACG GCTGGTTTGC AGGAACACAC AGGTGTAACA TCACCACTAT GGAGTGTTTG 
CCGGTTCCTG GGCATGGATT TGTGCTGGAC AAGTATAAAT GTCACTGCAA AAGTGGCTAT 
TATCATCCAA GCAGAGTTGC AGTGAATGGC TTTAAAAGGA AAGCAGGAGG GAAGGGGACC 
GAGGGTTTGT CCCATAACAG GGATGTTCCA GAAACTTCCA GCCACTGTCT GCCCTGCCAG 
GAAGGCTGTT CTTTCTGCAA GGACGATACG CCCTGCGTGG CCCGGGGTGA TGGAGCCCTG 
CGTATGGCGG TGCTCTCCTT CCAGGGCCTG TGCATGCTGG TGGATTTCAT CAGTATGGTG 
CTGCTTTACC ATTTCCGCAG GAACAAGAGT ATCAGAGCAT CAGGACTCAT TCTGCTGGAG 
GCCATCTTGT TTGGAGCTCT TCTCCTGTAC TTTCCGGTGG TGATCCTGTA CTTCCAGCCA 
AGTGTTTTCC GCTGTATCCT GCTGCGATGG GTCCGTCTGC TCGGGTTTGC CACTGTATAC 
GGAACAGTCA CTCTCAAGCT CTACAGGGTC CTCAAGGTGT TCCTGTCACG TACAGCCCAG 
CGGATCCCTT ACATGACCAG CTGGAGAGTG TTGCGTCTAC TGTGTATCAT CCTGTTGATC 
GTGTTATGGT TCGCAATAGC CTGGACCTCC GCTGTTTGTC AGAATCCAAA CAGGCACCTC 
GCTCTCATCA GTGTGGGCTT CACCACTGAC GGACTGCAGT TCAGCATGTG TCTGCTGGAC 
CGCTGGGACT ACATGATTGC TGTGGCTGAG TTCCTGTTTC TTCTTTGGGG TGTATATCTT 
TGCTTTGCGG TGAGGACCGT CCCATCGGCA TATCACGAAC CACGCTACAT GGCCATCGCA 
GTTCACAATG AGCTCATCCT CTCGGCCATA TTCCACATTT TAAGGTTCAC ACTTGCTCCT 
GAATTGCACC CAGACTGGAT GCTGATGCTT TTCTTTGCTC ATACACACTT AACGGTGACT 
GTCACTTTGG GGTTGCTGCT TGTGCCAAAG TTCTTGTTTG CGGGCACACA TCTAAGGGAT 
GACATTGCTA CAGAAGCTTA CGAAGATGAA TTGGACATGG GGCGGTCTGG GTCATACCTG 
AATAGCAGTA TCACATCTGC TTGGAGTGAG CACAGTCTGG ACCCAGAGGA TATCCGGGAA 
GAGCTGAAGA AACTCTACTC TCAACTGGAG ATTTACAAGA GGAAAAAGAT GCTGGCTAAC 
AATCCTCACC TGCAGAAGAA GCGAAGCTCC AAAAAGGGCC TAGGCCGCTC TCTCATGCGA 
CGCATCACTG AGATCCCAGA GACAATGGGA CGCCAGTGTA GCCGGGACGA CAAAGACCAA 
GGTGAACACG GGAGCAACCA TAACAGCATC TGTGTGCTGA GAAAGAATCC TTTTGATCCC 
TCACACTCTG TTAAGCCTGC TAAAGATGAA TCCTTAAAGA GCAAAGTTTT CTCCCTTAAA 
AAGTCCCACA GCAGTTACGA CCATGTTCGA GACCACAGTG AAGATTCCAG CAGTTCGGCA 
ACTGACAAGA TGGAAGTCAC AACCACAGAG GGCTCTCTTC TCGAAACCCT AATGGGAAAA 
AAGCTGGTCA AAAAGTCTTC TGAAAAGATT GATGTAGCCA GTGAGTCAAC AGAGTCGGTT 
CCCTTGGTGT GCAAGTCAGC CAGCGCTCAC AATCTGACAG CTGATAAGAA ACCTATTCAC 
CCCAGAACAT CCATGCTGCA GAAATCTCTA AGCGTTATTG CCAGTGCCAA AGAGAAAACT 
CTTGGTCTAA CAGGAAAGGC CCAGGGTGTA GAGGATTCAA CAAAGAAAGC CAATCAAAAG 
AACAGTGAGG GCAAGACCTC AGCTGAAGCT GAAGAAAAAG AAGGTTATCC GAAGATGATT 
GTCAGTCAAT CAGTGGAATA CAAGCAGACA GCAGCTAAAA CGGGTATAAT GAAGCCACAA 
ATGAGCGGAA GCCAGCCCTC CATTTGCTCA GAAACAGCCA AGGGGAAAGA CCTGTGTGAC 
CTCTCAGAAG TGTGCCCTTG GGAAGTGGAG GATTTGCCAA CTCCCTCCGA AAATAAAGTC 
CAGAAACATG TGTCTATTGC TCCTGAGCAA ACAAACACAG TTCGTGGAGG TAGCAGCAAG 
ACGACAAAGT CACAACAGCA AAAGCAAAAA GGAGCAGAGC AGTCACCTTC TGCGGCCAGG 
CATCCAAACA AGAAAGCTGC AGATAGAGCA GACATATGTC CGTGGGAGGA AGGAAACGAG 
GATCATGGTC AGCCAACAGG GCCAAAACCT CACTCCAATA CTAAGTCTGC CATGTCTCAA 
CAACAGGCTT CCAGAGAGGT TGCCAAGGTT TTAAAAGCAG ACGTCTGTCC ATGGGACTTT 
GAGGAAGTAT CAAGCAAGCC AAAGGACTCC CCTCGCTCCC CTGATCAGAG CAGACCAAAA 
AAGGGCACCA CACCAACTGA GATCAAAGGC AAAAAAAGCT CCTCGCCTAC TGAAGTCAAG 
GGCAAGGGTA CTACGCCTAC AGAGGTCAAA GCAAAGAGTA TTCTCTCTGA ACCTACTAAA 
TCCACAGGCA GCTCACTGCA GCCGCCCTCT AAAGCAGACG TGTGTCCATG GGACTTTGAG 
ACTATGTCAA GTCCCACCTC TGAAAAGACC CCTTGTTCTT CTCAGGTTCC TAAATCAAAG 
GAGACCCATG CTATAAAGAA GGGAAATTCC CCTGTCAGAT TATTAGAGTT GGAAAGAGGA 
AACACAAAGG GTCCAGATGA GGACAAAATG AAATCAAACA CAAAGTTCAG CTCTGGCAAG 
ACAAAAGAAA GACCCGTGAG CCAGTCTAAA ATAATGGCCG AGGTTTGTCC GTGGGATGTT 
GATAGTAGTC AGGGAAATGT TTCAATGGAA AAACAAAAAA GCCCAAATGT TGCATTAGCA 
AAAAGCAAAC AAGCAGACGT ATGTCCTTGG GATTTTGAAG AGGCAGCAAC TAGTAAGGAG 
GCCAACAGGG GCACCGGTGG CAAACAGAAA AGTTCAAATT CTAGTGGTGT TAAAACGGCA 
GATGTCTGTC CATGGGACTT TGATGATCAA GCATCGACAA AAAAGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162592.1
CDS	876..4886
Translation	MAIIRISLLLQVGFVIGSNYRYVEHDRDLKGKLPIYNTHSNQHAHLRKPHSGLVQKIEEQVPKVVTAFLHTGDSSTLKHANCSRRYELAVLRGRTRGDAHHSMRSVLDTVLHATNFLNMILQTNRSRNHSPRHADMEWYNALVRSILEGDLKIHRAVVTLSTESPPEGPPVYLQATRAGGEIILQDLSNITHRMLRNRTADTEWYHEHKHKKKTHLQKRVLSQDFASFDASVRSGESYITEKTQIHWSAPYLECENGNFIPRWLLTLSAGFYGLKPNRNPDFRGVVRVDVSLQDVDIDQCSADGWFAGTHRCNITTMECLPVPGHGFVLDKYKCHCKSGYYHPSRVAVNGFKRKAGGKGTEGLSHNRDVPETSSHCLPCQEGCSFCKDDTPCVARGDGALRMAVLSFQGLCMLVDFISMVLLYHFRRNKSIRASGLILLEAILFGALLLYFPVVILYFQPSVFRCILLRWVRLLGFATVYGTVTLKLYRVLKVFLSRTAQRIPYMTSWRVLRLLCIILLIVLWFAIAWTSAVCQNPNRHLALISVGFTTDGLQFSMCLLDRWDYMIAVAEFLFLLWGVYLCFAVRTVPSAYHEPRYMAIAVHNELILSAIFHILRFTLAPELHPDWMLMLFFAHTHLTVTVTLGLLLVPKFLFAGTHLRDDIATEAYEDELDMGRSGSYLNSSITSAWSEHSLDPEDIREELKKLYSQLEIYKRKKMLANNPHLQKKRSSKKGLGRSLMRRITEIPETMGRQCSRDDKDQGEHGSNHNSICVLRKNPFDPSHSVKPAKDESLKSKVFSLKKSHSSYDHVRDHSEDSSSSATDKMEVTTTEGSLLETLMGKKLVKKSSEKIDVASESTESVPLVCKSASAHNLTADKKPIHPRTSMLQKSLSVIASAKEKTLGLTGKAQGVEDSTKKANQKNSEGKTSAEAEEKEGYPKMIVSQSVEYKQTAAKTGIMKPQMSGSQPSICSETAKGKDLCDLSEVCPWEVEDLPTPSENKVQKHVSIAPEQTNTVRGGSSKTTKSQQQKQKGAEQSPSAARHPNKKAADRADICPWEEGNEDHGQPTGPKPHSNTKSAMSQQQASREVAKVLKADVCPWDFEEVSSKPKDSPRSPDQSRPKKGTTPTEIKGKKSSSPTEVKGKGTTPTEVKAKSILSEPTKSTGSSLQPPSKADVCPWDFETMSSPTSEKTPCSSQVPKSKETHAIKKGNSPVRLLELERGNTKGPDEDKMKSNTKFSSGKTKERPVSQSKIMAEVCPWDVDSSQGNVSMEKQKSPNVALAKSKQADVCPWDFEEAATSKEANRGTGGKQKSSNSSGVKTADVCPWDFDDQASTKKA

Target ORF information:

RefSeq Version	XM_005162535.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio G protein-coupled receptor 158a (gpr158a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162535.3

ORF Insert Sequence:

ATGGCCATAA TCCGGATATC GTTACTCTTG CAAGTGGGAT TTGTTATAGG ATCAAATTAT 
AGATATGTGG AACATGACCG GGACCTGAAA GGAAAGCTGC CCATTTACAA CACGCACAGC 
AACCAGCACG CGCACCTCCG GAAGCCCCAT TCGGGTTTAG TGCAGAAGAT CGAGGAGCAG 
GTACCCAAAG TAGTGACTGC GTTTCTGCAC ACGGGGGATT CCTCCACCTT AAAGCACGCC 
AACTGCTCCA GGAGATATGA GCTGGCGGTG TTGCGGGGAA GGACGCGCGG AGACGCGCAT 
CACTCCATGC GCAGCGTCCT GGACACGGTC CTTCACGCCA CAAACTTCCT CAACATGATA 
TTACAAACAA ACCGATCCAG AAATCACAGC CCCAGACATG CAGACATGGA GTGGTACAAC 
GCGCTGGTCA GGAGTATCCT GGAGGGAGAC CTCAAGATCC ACCGAGCGGT GGTCACCTTG 
AGCACAGAAT CCCCTCCTGA AGGACCACCT GTTTACCTTC AGGCTACACG GGCAGGTGGA 
GAGATCATTC TGCAGGATTT ATCCAACATT ACCCATCGGA TGTTGAGAAA CAGAACGGCT 
GACACCGAGT GGTATCATGA GCACAAACAT AAAAAGAAGA CACATCTGCA AAAACGAGTG 
CTGAGTCAAG ATTTTGCCTC GTTTGACGCG TCCGTGCGCA GTGGAGAAAG TTATATTACC 
GAAAAAACTC AGATACACTG GTCAGCTCCG TACTTGGAGT GCGAGAATGG GAATTTCATA 
CCCAGATGGC TCTTGACACT GTCAGCTGGC TTCTATGGCC TTAAACCCAA CAGGAACCCA 
GACTTCAGGG GTGTTGTTCG AGTGGACGTC AGCCTGCAGG ATGTGGATAT TGACCAGTGC 
TCTGCAGACG GCTGGTTTGC AGGAACACAC AGGTGTAACA TCACCACTAT GGAGTGTTTG 
CCGGTTCCTG GGCATGGATT TGTGCTGGAC AAGTATAAAT GTCACTGCAA AAGTGGCTAT 
TATCATCCAA GCAGAGTTGC AGTGAATGGC TTTAAAAGGA AAGCAGGAGG GAAGGGGACC 
GAGGGTTTGT CCCATAACAG GGATGTTCCA GAAACTTCCA GCCACTGTCT GCCCTGCCAG 
GAAGGCTGTT CTTTCTGCAA GGACGATACG CCCTGCGTGG CCCGGGGTGA TGGAGCCCTG 
CGTATGGCGG TGCTCTCCTT CCAGGGCCTG TGCATGCTGG TGGATTTCAT CAGTATGGTG 
CTGCTTTACC ATTTCCGCAG GAACAAGAGT ATCAGAGCAT CAGGACTCAT TCTGCTGGAG 
GCCATCTTGT TTGGAGCTCT TCTCCTGTAC TTTCCGGTGG TGATCCTGTA CTTCCAGCCA 
AGTGTTTTCC GCTGTATCCT GCTGCGATGG GTCCGTCTGC TCGGGTTTGC CACTGTATAC 
GGAACAGTCA CTCTCAAGCT CTACAGGGTC CTCAAGGTGT TCCTGTCACG TACAGCCCAG 
CGGATCCCTT ACATGACCAG CTGGAGAGTG TTGCGTCTAC TGTGTATCAT CCTGTTGATC 
GTGTTATGGT TCGCAATAGC CTGGACCTCC GCTGTTTGTC AGAATCCAAA CAGGCACCTC 
GCTCTCATCA GTGTGGGCTT CACCACTGAC GGACTGCAGT TCAGCATGTG TCTGCTGGAC 
CGCTGGGACT ACATGATTGC TGTGGCTGAG TTCCTGTTTC TTCTTTGGGG TGTATATCTT 
TGCTTTGCGG TGAGGACCGT CCCATCGGCA TATCACGAAC CACGCTACAT GGCCATCGCA 
GTTCACAATG AGCTCATCCT CTCGGCCATA TTCCACATTT TAAGGTTCAC ACTTGCTCCT 
GAATTGCACC CAGACTGGAT GCTGATGCTT TTCTTTGCTC ATACACACTT AACGGTGACT 
GTCACTTTGG GGTTGCTGCT TGTGCCAAAG TTCTTGTTTG CGGGCACACA TCTAAGGGAT 
GACATTGCTA CAGAAGCTTA CGAAGATGAA TTGGACATGG GGCGGTCTGG GTCATACCTG 
AATAGCAGTA TCACATCTGC TTGGAGTGAG CACAGTCTGG ACCCAGAGGA TATCCGGGAA 
GAGCTGAAGA AACTCTACTC TCAACTGGAG ATTTACAAGA GGAAAAAGAT GCTGGCTAAC 
AATCCTCACC TGCAGAAGAA GCGAAGCTCC AAAAAGGGCC TAGGCCGCTC TCTCATGCGA 
CGCATCACTG AGATCCCAGA GACAATGGGA CGCCAGTGTA GCCGGGACGA CAAAGACCAA 
GGTGAACACG GGAGCAACCA TAACAGCATC TGTGTGCTGA GAAAGAATCC TTTTGATCCC 
TCACACTCTG TTAAGCCTGC TAAAGATGAA TCCTTAAAGA GCAAAGTTTT CTCCCTTAAA 
AAGTCCCACA GCAGTTACGA CCATGTTCGA GACCACAGTG AAGATTCCAG CAGTTCGGCA 
ACTGACAAGA TGGAAGTCAC AACCACAGAG GGCTCTCTTC TCGAAACCCT AATGGGAAAA 
AAGCTGGTCA AAAAGTCTTC TGAAAAGATT GATGTAGCCA GTGAGTCAAC AGAGTCGGTT 
CCCTTGGTGT GCAAGTCAGC CAGCGCTCAC AATCTGACAG CTGATAAGAA ACCTATTCAC 
CCCAGAACAT CCATGCTGCA GAAATCTCTA AGCGTTATTG CCAGTGCCAA AGAGAAAACT 
CTTGGTCTAA CAGGAAAGGC CCAGGGTGTA GAGGATTCAA CAAAGAAAGC CAATCAAAAG 
AACAGTGAGG GCAAGACCTC AGCTGAAGCT GAAGAAAAAG AAGGTTATCC GAAGATGATT 
GTCAGTCAAT CAGTGGAATA CAAGCAGACA GCAGCTAAAA CGGGTATAAT GAAGCCACAA 
ATGAGCGGAA GCCAGCCCTC CATTTGCTCA GAAACAGCCA AGGGGAAAGA CCTGTGTGAC 
CTCTCAGAAG TGTGCCCTTG GGAAGTGGAG GATTTGCCAA CTCCCTCCGA AAATAAAGTC 
CAGAAACATG TGTCTATTGC TCCTGAGCAA ACAAACACAG TTCGTGGAGG TAGCAGCAAG 
ACGACAAAGT CACAACAGCA AAAGCAAAAA GGAGCAGAGC AGTCACCTTC TGCGGCCAGG 
CATCCAAACA AGAAAGCTGC AGATAGAGCA GACATATGTC CGTGGGAGGA AGGAAACGAG 
GATCATGGTC AGCCAACAGG GCCAAAACCT CACTCCAATA CTAAGTCTGC CATGTCTCAA 
CAACAGGCTT CCAGAGAGGT TGCCAAGGTT TTAAAAGCAG ACGTCTGTCC ATGGGACTTT 
GAGGAAGTAT CAAGCAAGCC AAAGGACTCC CCTCGCTCCC CTGATCAGAG CAGACCAAAA 
AAGGGCACCA CACCAACTGA GATCAAAGGC AAAAAAAGCT CCTCGCCTAC TGAAGTCAAG 
GGCAAGGGTA CTACGCCTAC AGAGGTCAAA GCAAAGAGTA TTCTCTCTGA ACCTACTAAA 
TCCACAGGCA GCTCACTGCA GCCGCCCTCT AAAGCAGACG TGTGTCCATG GGACTTTGAG 
ACTATGTCAA GTCCCACCTC TGAAAAGACC CCTTGTTCTT CTCAGGTTCC TAAATCAAAG 
GAGACCCATG CTATAAAGAA GGGAAATTCC CCTGTCAGAT TATTAGAGTT GGAAAGAGGA 
AACACAAAGG GTCCAGATGA GGACAAAATG AAATCAAACA CAAAGTTCAG CTCTGGCAAG 
ACAAAAGAAA GACCCGTGAG CCAGTCTAAA ATAATGGCCG AGGTTTGTCC GTGGGATGTT 
GATAGTAGTC AGGGAAATGT TTCAATGGAA AAACAAAAAA GCCCAAATGT TGCATTAGCA 
AAAAGCAAAC AAGCAGACGT ATGTCCTTGG GATTTTGAAG AGGCAGCAAC TAGTAAGGAG 
GCCAACAGGG GCACCGGTGG CAAACAGAAA AGTTCAAATT CTAGTGGTGT TAAAACGGCA 
GATGTCTGTC CATGGGACTT TGATGATCAA GCATCGACAA AAAAGGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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03-6811-6573
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オンライン注文

gpr158a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報