lama1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	lama1
Entrez Gene ID	569971
Full Name	laminin, alpha 1
General protein information	Preferred Names laminin, alpha 1 Names laminin subunit alpha-1 bal bashful zmp:0000000656
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001034986.1	NP_001030158.1	laminin subunit alpha-1 precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Lama1	NP_001101707.2
Gallus gallus (chicken)	LAMA1	NP_001186735.1
Xenopus tropicalis (tropical clawed frog)	lama1	XP_004915294.1
Pan troglodytes (chimpanzee)	LAMA1	XP_001141549.1
	LAMA1	XP_001118617.2
Canis lupus familiaris (dog)	LAMA1	XP_005623125.1
Homo sapiens (human)	LAMA1	NP_005550.2
Danio rerio (zebrafish)	lama1	NP_001030158.1
Bos taurus (cattle)	LAMA1	XP_002697843.2
Mus musculus (house mouse)	Lama1	NP_032506.2

Gene Ontology
Bibliography

Related articles in PubMed

Hedgehog signalling acts upstream of Laminin alpha1 transcription in the zebrafish paraxial mesoderm.
Pickering J, Cunliffe VT, Van Eeden F, Borycki AG
Matrix biology : journal of the International Society for Matrix Biology6258-74(2017 Oct)

NgAgo-based fabp11a gene knockdown causes eye developmental defects in zebrafish.
Qi J, Dong Z, Shi Y, Wang X, Qin Y, Wang Y, Liu D
Cell research26(12)1349-1352(2016 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Loss of lama1 leads to divergent effects on focal adhesion assembly in a spatiotemporally-specific manner. Laminin is required for multiple steps of optic cup morphogenesis, including optic stalk constriction, invagination, and formation of a spherical lens. In lama1(UW1) mutants, loss of epithelial polarity and altered adhesion lead to defective tissue architecture and formation of a disorganized retina.
Title: Loss of laminin alpha 1 results in multiple structural defects and divergent effects on adhesion during vertebrate optic cup morphogenesis.

tag1, lama1 and vangl2 participate in a common mechanism that integrates signaling between the FBMN and its environment to regulate migration.
Title: The cell adhesion molecule Tag1, transmembrane protein Stbm/Vangl2, and Lamininalpha1 exhibit genetic interactions during migration of facial branchiomotor neurons in zebrafish.

two guidance cues, TAG-1 and laminin-alpha1, influence the behavior of growth cones during axon pathfinding in vivo
Title: Transient axonal glycoprotein-1 (TAG-1) and laminin-alpha1 regulate dynamic growth cone behaviors and initial axon direction in vivo.

The phenotypic and genetic analyses of a recessive, larval lethal zebrafish mutant, bal(a69), characterized by severe eye defects and shortened body axis, is reported.
Title: Mutations in laminin alpha 1 result in complex, lens-independent ocular phenotypes in zebrafish.

Laminin alpha 1 is needed for lens development in zebrafish embryos.
Title: laminin alpha 1 gene is essential for normal lens development in zebrafish.

The following lama1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lama1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01538	NM_001034986.1 Latest version!	Danio rerio laminin, alpha 1 (lama1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lama1 ( NM_001034986.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001030158.1 Danio rerio laminin, alpha 1 (lama1), mRNA.

Price & Availability↑

CloneID	ODa01538
Clone ID Related Accession (Same CDS sequence)	NM_001034986.1
Accession Version	NM_001034986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-31
Organism	Danio rerio(zebrafish)
Product	laminin subunit alpha-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ131910.1. On Oct 22, 2005 this sequence version replaced XM_693392.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ131910.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA898401, SAMEA898403 [ECO:0000348] ##Evidence-Data-END##

ATGATGGAGA TGAGGATGAA GATGCTGTGG CTGTTAGCGC TGGTTTTCTC CTCAGTGATT 
TGTGTGGAGG CTCAGCAGAG AGGTTTGTTT CCTGCAATCT TGAATCTCGC CAGCAATGCC 
GAAATCAGCA CCAACGCCAC CTGTGGCGAT CCCGATCCCG AGATGTTCTG CAAACTGGTG 
GAGCACGTTC CTGGAAGACG GATCCGCAAC CCGCAGTGTC GCATCTGTGA CGCAAACAGC 
CAGAATCCCA AAGAGCAACA TCCAATTACT AACGCCATCG ATGGAACCAA CCTGTGGTGG 
CAGAGCCCGA GCATCAAGAA CGGCCGTCAG TTCCACTGGG TCACAGTCAC GCTGGACCTG 
CGACAGGTGT TCCAGGTGGC GTACATCATC ATTAAAGCGG CGAACTCTCC TCGACCTGGA 
AACTGGATTC TGGAGCGTTC CCTGGACGGC GTGAACTTCC AGCCGTGGCA GTTTTACGCC 
ATTAGCGATA CTGAGTGTTT GACGCGCTAC AACATCACAC CGCGCATCGG CCCGCCGACA 
TACAAGAGAG ACGACGAGGT GATCTGCACA TCGTACTACT CTCGCCTGGT GCCGCTGGAG 
CATGGAGAGA TCCACACGTC TCTGATTAAC GGCCGTCCCA GCGCTGACGA TCTGACACCG 
GAGCTGCTGG AGTTCACATC GGCGCGGTTT ATACGCCTGC GCCTGCAGAG GATTCGGACG 
CTCAACGCAG ACCTGATGAC CCTCAGCTAC CGAGACCCCA AAGACGTGGA CCCCATCGTC 
ACCCGACGGT ATTATTACTC CATCAAGGAC ATCTCAGTGG GCGGGATGTG CATCTGCTAT 
GGCCACGCCC AGAGCTGCCC CTGGGACCCT GTTACCAAGA AGCTGCAGTG TGTGTGTGAA 
CACAACACTT GTGGTGAGAG CTGTAATGAG TGTTGTCCTG GATACCATCA GGAGCCATGG 
CAGCCGGGAA CGCTCTCCGA CGGCAACACT TGCGAGAAGT GTAATTGTCA CAACAAAGCC 
GACGACTGTT TCTACAACCA GACGGTGGCG GATCTGAAGC TGAGCATGAA CACACACGGC 
CAGTTCACAG GAGGAGGAGT GTGTGTGAAC TGCAGACACA ATACAGCGGG AGTGAACTGT 
GAGACCTGTG CGGACGGATA CTACAGACCC CACCAGGTGT CTCCGTATGC AGAAGAGCCG 
TGTGTGGAGT GTCAGTGTGA TATGAGGGGT TCAGTGAGTC CTGTGTGCAT CAGAGATGAT 
AATCACGCAA ACCCAGACGC AGGTCTGTCT CCAGGTCAGT GTGTGTGTAA GGAGGGTTTT 
GCGGGAGAGA AGTGTGACCG ATGTGCGTTC GGCTTCAGAG ATTTCCCGGT GTGTTCTCGC 
TGCGAGTGTA ACCTGGATGG GAGCCACAAC ACCGACCCCT GTATGGAGTG TGTGTGCAAG 
GCGAATGTGA TGGGCTCTCA CTGTGACCTG TGTAAGCAGG GCTTCTATAA CCTGCAGGCC 
AGCAATCCTG AAGGCTGCAC CGAGTGTTTC TGCTTCGGTG TGTCAGATGT GTGTGAGAGC 
TCCACCTGGT TCAGCAGCTC GGTGGTGCAC AGAGACGGCG TCCTCCATCG CCTGCACCAG 
ACCTCCATCA GCTTCTGGTC TCCAGTTTCT GATGAAAACC TGATCATCTC CAACAGATCC 
ACAGATGCAG ACCCCTCCTC TGTGTGGACA TGGGCCGCTC CGGAGCCTTT CCTCAATAAC 
AAGCTGACGT CATATGGAAG TTTCTTGAAC TACAGTGTGG CGTATGACAC TTCTGAGGAG 
AACGTGGACA AAACACTGCG CTCACACTTC AGCGTAATTA TTGAGGGTAA CGGGCGCACG 
CTGAGGCAGG CGCAGTCGGC TCGTCTGCTG CTGAACGCTC ACACACACCA GCGTGTGTTT 
GTGCAGATGC TTCCGCAGAT CTTCATAGAC TCACACAGCG GCAGGAGCGT GCAGAGAGAC 
GAGCTGATGA CGGTGCTGGC GGACGTTGCT GCGCTCAGGG TTCGAGCCGA GCTGGAGGAC 
AGCGCTGAGG GAACGCTCAG GTTGAGTCAC GTGTCTTTAG GTGTTGGAGA CTCAAACTCC 
GATGTGTCTA AAATCTCTCT AGATGTGGAG CACTGTGAGT GTCCGTGGGG ATATTCAGGC 
ACTTCCTGTG AGTTGTGTAT TCCTGGCTTT TATCGGGTCG GTGGGATTTT GTTCGGAGGA 
AACTGTCTTC CGTGCGAGTG TAACGATCAC GCGACGGAGT GTGACATCAA CGGAGAGTGT 
CTGGGCTGTG CGCACAACAC CACGGGGCCT CACTGTGATC AGTGCCTGCC GGGTTTCTAT 
GGTGACGCGA GCGAGGGGAC GCCGGACGAC TGCCAGAGAT GCTCCTGCCC GCTGACACTG 
GCGTCAAACA ATTTCAGCCC CACGTGTCAG CTGCAGGCTC CCGGTGAATT CATCTGTGAT 
CAGTGCCAGT CTGGATACAC CGGAGACAAG TGCCAGAGGT GTGCTGATGG GTATTTTGGT 
GAGCCGGTGG TCCCTGGTCA GCGCTGCTCC CCCTGTGAGT GTAACGGGAA TGTGGACCCA 
TCTGAAGCGG GACGCTGCGA CACCCACACT GGGGAGTGTC TGAAGTGTGT AGGGCATACG 
GCCGGGCCAC ACTGCGAACG CTGCCGGGAC GGATATTATG GAGACGCCAT CCAAGAGAAG 
AACTGCCAGG CCTGTGGTTG TTATAGTAAC GGCTCTCTGT CCACCATCTG TAACCTGATC 
ACGGGTCAGT GTGAATGTAA ACACAATGTG GTCGGAAAAA CCTGCGACCG CTGTCAGGAG 
GGTTATCACG GTATTAACAG TGGTGAAGGC TGCAGACCGT GTGAATGTAA CCAATCAGGC 
GCTCTGTCTG CGTCATGTGA TGAGGAGGGG CGGTGCCAGT GCATAACTGG GGTAACGGGT 
GACAAATGTG ATCGATGTCA CCATGGATAC TACAACTTCA AAGAGAACGG ATGCACAGCA 
TGCGACTGCG CACACACTCA TGGAAACTGC AACGGTCAGA CGGGCGAGTG TATCTGTCCT 
CCACACACAC ACGGGCTGAA GTGTGAACAG TGTGATGAAG GTCACTGGGG TCACGACGGA 
GTGTCCGGCT GTAAGGTGTG TAACTGCAGT GTTGTGGGCT CCAGCTCGTC TCAGTGTGAT 
CTGTCCTCGG GTCAGTGTGT GTGTGCGCTG CAGTTCTCCG GCCTCACATG CGACCGATGC 
GCTTTAGGCT ACAGAAACTT CCCACAATGC TCTGCCTGCG ACTGCAACCC GAACGGCACG 
CGTGCGCAGT TCTGTGACGA GGCACTAGGA GTGTGTGGCT GCGAGGATCA CGGGCAGTGT 
TCCTGCAAGG ATAATGTGGG CGGCAGAGGC TGTGACGAGT GTAAGAGCGG CTCCTTCGGG 
CTCTCGGCCC TCAATCCGGC CGGCTGCAGC CCCTGCTTCT GCTTTGGCCT TTCAGACGTG 
TGTGAGGAGC TGAGCGGATT GGTGCGCGCG CCGATTGTTC TGGGTTCATC TCCGGCTCTG 
CTGCGTGTAG TGTCTCAGAG TGACCTGCAG GGGACGCTGG AGGGCGTTTA TTACAGCGAT 
GACGAGATGC TGCTGGACGT GGATCAGCTC AACAAACTCT CTCTCCTCAC CGGACCGTAT 
TACTGGAGAC TCCCACAGAA ATACAACGGC AACAAGCTGC TGTCGTATGG AGGAAGACTC 
TCCTACACCC TCACCTTCTT CGCTCAGGAC GGCGTGGGAT TGGCCAATCA GGAGCCTCAG 
GTGCTGATGA GAGGAGGACA CCTGCGTAAG CTGGTCATCT ACACTGATGC TAGCGCTCCT 
GCTAACGGCA TCCGCAGCAC ACAGGAGCAC GCTCTCACCG AGCACAAGTG GAAATACTTC 
AACTCAGTGT CTGATGAAGC CGTGAGCCAC GCTGACTTCA TGTCTGTGCT GAGTAACCTG 
GAGTACGTCA TCATTAAAGC CTCGTACGGG TCCGGACTGC AGCAGAGCAG GATCTCCAAC 
ATCTCCATGG AAACTGCATT GGAGGCAGAT GAGCTTCCTG AAGGTGGAGA TGTGGCGCGG 
TTGGTGGAGA TTTGTGAGTG TCCGCCGGGA TACGCTGGAC TGTCCTGCCA GGAGTGTGCT 
CCAGGATATT ACCGGCAGGC TGTATCTGAG CTCAACATGA AGGGCAGAAA TCGGCCGCTC 
ATTCAGCCCT GTGTGCCCTG CAGATGCAGC AACCACAGCC AGTCATGTGA CCTGCACACC 
GGCCAATGCC TGGGCTGTCA GCACAACACC GCTGGAGAAC ACTGCCATGT TTGCGCTGCA 
GGGTATTATG GGAAGGTGCA GGGGTCGGTC AGTGACTGCT CTCTGTGTGC CTGCCCACTG 
CGGGGTCAAA GTTTCAGCTC AACATGTGTG TTAGAAGGTG CTGGAGACTT CCGCTGCGAC 
CGCTGTGAGG AGGGATATGA GGGACGATAC TGTGAGAGGT GTGCAGTGGG GTATTTCGGG 
AACCCGTCGG AGCCGGGTGG CCGCTGTCAG GTGTGTGCGT GCAGTGAGTC GGGCTCAGTG 
CACAGTGTGT GTGACGCACA CACAGGCCGC TGCGAGTGTA AACCTGGGGT CAGAGGTCAC 
CTGTGTGACC AGTGTGAGGA GAGGCATGTG CTGGTCCACG AGCAGTGTGT GTCATGTAAT 
GACTCCTGCA CTGGTGTTTT GCTGGATGAT CTGGATGCTT TAGACGTGTC CATCACATCT 
GTGAACCTGA CGGGTGTCAT TCTGGCCCCG TACAGCCAGC TGATGACCAT CCAGAACCAG 
ACCCGAGAGC TCACGAGCCT GATGTCGTGG AACCAAACTC CAGATTATCA GCTGACCGCT 
GGAGAAGAAC ACTCGAGGAA CCTGAGCGGT CAGATCACTG ACCTTCAGCA GCAGGTCCAG 
AGTGTGTTTG AAGACAGTGG TGATGTGCTG CTGACCTCAG AGCAGAGATT TACTCAAGGG 
AAACAACTGC TGGAGCTCAT CAACAACATT CACTCTGCCA TCCACGGTCT GGTGGAGCAG 
GTGAGTTCAG TGAACGTGAC TCTGGATGAA GGTCTGGATG AAGCCAACAG CACATTCCTG 
CTGGAGGAGA CCAGCGAAAC CCTGGAGAAA ATCAGGAGCT TTAATCTGAC GCACTGCAGG 
AACGCTGCGG AGATCCAGCT CAGCTCTGCA GTGTCTGTGC TGGAAAAGGT CGAGGATGAG 
ATTTCAAAGC CTTACAGAAA TAACCAGATC AGACGGGAAA ACATCTCCAC AACACTCAAC 
ACACACAACC TGAGTCTCCA GCAGGTCCAG GAGAGCCTTT ACACGGCTAA AACGCACAAC 
AACCAGAGCC GACGCCTGCT CCAGGACATC CAGAGCAACA CACACACACT CACCATCTCC 
AGGAACAATG TCAGCAGTCT GAACCATGAA CTAGAAACAC AAGTACAGGA AGCTCAAGAC 
CTGCTGACCG ACGCCCTCAA TATTGCAGAA GACATGGACA ACGGGGCGAC AACTCTGGAT 
GAGCTGAAGA ATGATCTGGA ATTGTGGAGT CCGTCTCTCA GGAAGCATGT GGACTCTTTA 
GTCATGGATT TGACCAAAAG AGATGCTCTT CAGCTGGTTT ACAGGGCTGA GGATCACGCA 
CAAGCTCTCA GCAAACAAGC ACAAGCCCTG AACAGTTCTC TGTCTGAGGT CAGCTCTGCA 
TCAGTTAACA TCTCCATCGC CACCCGAACC AATGCCAACA TCAGAGAAAA CATACAGCTG 
GCGGCAGATC TGGCCCTCGC AGCCAATCAG AACGCAAGCT CCGCCCTCAA TATGACGTCA 
CTGACGGAGC AGGAGAAGAG GAGGCTGCAG AAGAGCTCAG AGATTCTGGA CAGAGCTGCA 
CTTCTGAAAA ACCACACCGA CGGCCTCATG CAGAATGTCA GTGCAGTGAC CAGCAGGTTG 
AGTCTGGTTG CAGCAAACAT CCAGAACTTC ACACAGATGC TCCCAGAAGC CCTCGGCATC 
CTCAGAAACC TTCCTGAAGG CAGCAGAGCT GAAATCCTGC AGGTGAAGCA GCAGACAGAA 
GCAGCGAATG CGTCTTTACA GGAAGCTCTG GAGCGTTTGC GGGATTATCG CCTAAAGCTG 
GACGAGTCTT CATCAGCTCT GGGGTCAGCC GATAACAGCA CACAGAGGAC CAATCAGCTG 
CTCAGAGACT CACAGGACAC CGCAAACGCT GCTGGATCCC GCCTGAGCGA GGCTGATGTG 
AAAGCTGATC GTCTGCTGGA GCGTCTGAAG CCTCTGCAGA CTCTGGGAGA AACGCTGAGC 
AGAAACCTGT CCGACATCAG AGAACTCATC AGTCAGGCGC GCAAACAGGC TGCATCGATT 
AAAGTGGCCG TCTCTGCAGA CCGCAACTGT GTACGCGCGT ACAGACCTGA GGTGACGTCC 
AGCAACTTCA ACACACTCAC ACTGACCATG AAGACCAGTG AGCCCGACAA CCTGCTCTTC 
TACATGGGCA GCAGCTCCAG CGAGGATTTC ATGGCTCTAG AAATGCATGA TGGGAAGGTG 
TCCTTCCTGT GGGACGCCGG TTCAGGACAC AACAGACTGG AGTATCCGGA CGTTCAGATC 
AACAATGATA AATGGCACAG GATCAATGCC ACGCGGTTTG GGAGACACGC TACACTGACG 
GTTCAGCAGA CGGACTCGGA GCCGCTGCCC GCCGTAAAGA GCAGCGCCGC TGGATCTGCC 
ACTGTGATGG ACGTCAATAA ACACACATGG GTGTATGTGG GCGGCCTGGG TGGACAGGTG 
AAGAAATCTC CAGCAGTGAA AATAACCCAT TTCAGAGGCT GTATGGGTGA AGCGTTGCTC 
AACGAGAACA ACATCGGCTT GTGGAATTAT GCTGAGAGAC AGGGCGAGTG CGGAGGCTGC 
TTCAGGAGTC CTCGCACTGA AGACACCTCG TTTCACTTCG ACGGCAGCGG CTTCTCTGTG 
GTGGAGAAAT CTCTGCGCTC CATGTCCACC AGCGTGGTGA TGTTCTTCAA GACTCTGTCC 
CCCAACGGCC TGCTGCTGTA TCTGGCCTCC AACGGCACGA GAGACTTTCT GTCCATCGAG 
CTGGTGGAGG GAAAGGTTCA TCTGACCTTT GAACTGGGCT CCGGAGCGCT GACCATGACC 
TCCAGCAGAA CATACAACAC CGGATCCTGG TACCGAATCG CCCTGCAGAG GAACAAGCGC 
AAAGGTCATT TATCTGTCAT GGCTGCTTCA AACCCGTCCG AGCGTGAGGT GCTGGAGGCG 
GAGGCTCCGG GTCAGGCTTC TGACCTCAAC CGATCCGACC TTGACCCCAT TTACATCGGT 
GGATTGCCAG CCTCTCGACC AATCAGGAGG CAGGTTGTCG CTCGCTCTTT CGTCGGCTGC 
ATGAAGAACG TGGAAATCGC ACGAACAAAC TTCGACCTGC TCAGAGAGGC GTACGGCGTG 
AAGAAAGGCT GCGTCCTCAA GCCCATTCGC AGTGTGTCAG TGTTTGGCGG AGGGTTTCTG 
GAGATGCCGG CGGTGCTGCT TTCCTCTCAC ACTGAAATCA TGAGCACCTT CTCCAGCAGA 
CGTGACAGCG GAGTCATCCT GGCCGGATTC AGCCGCTCAT CCAGACACAC ACAACAGCCG 
CTGTTACTGC TGCTGCTGGT GTCTGGAGGT CTGGAGCTGC GTGTGAGTGT GTCTGATGGA 
GCAGCGGTGC AGAAAGCAGA ACTCAGAGGA TCCTTCAGTG ACGGACATGA ACACTCACTC 
ATCCTGCAGA GGAACAAACG GACGATCACA GTGGTTGTGG ATGAGGGCAT CCAGGCCTCG 
CTGAAGCTGC CGGCGTCTGC AGAGAAAAGC CTGACTCTGG AGCGGCTCTA CATCGGCGGC 
GTTCCTCCAG CAGAGGGAGA CACACACACA CTCACACCTG CCTTCTACGG CTGCATTAGG 
AACACTGCGG TCGACGGCAT GTTGTTGGAT CTGTCCGCTG CGCTGCGCTA CGAGCAGGTG 
GATATGGACA GCTGTTTGCT GGAGCCGCGG CCCAGACGAG TACTGCTGCC TGATGACCCG 
GAGCCGACAT CTGACCCCGA CACACGACCT CTGACCCCTG CAGCAGCGGA GCTGAGTGCG 
CTCACACCAA ACAGCGGCAG CTGTGCATCA GCGGACCACA CACAGACCAT CCCAGAATCC 
CACCAGTTCG GCCTGTCTCG ACACAGTCAC CTAATCATGG GTTTCAAAAA CCGGACGGTC 
AGGACCAGTT TTGCAGTGAA GCTGTCGGTG CGCACGTTTG CTCACAGCGG CGTTCTGTTC 
TACATGGCGA ACACAAACCA GCAGGATTAC GCAGTTCTGC AGCTGCAGGC CGGACGACTG 
CATCTGTCCT GTGACCTGGG CAAAGGAGCG GCCAGCGCCA CGCTCAAAAA CACCATCAGT 
GACGGACGCT GGCACACGGT GAAGGCCGAG TTCAGTAAGA AGACTGTTGT GGTCAGTGTG 
GACGGGATTG AATCTGACCA CATCTCCACT AAAGGACACA CACTAGACGT CGAGGGGAAG 
TTGTACGTCG GTGGACTTCC TCCCACATAC ACCGCCAAGA GAATCGGAAA CGTGACCCAC 
AGTGTGGCGG CGTGTGTGCA GGACCTGACG TTTAACGGTG TCCCAATGAA CCTGAATAAT 
CCGCTGTCAT CTCACGCCAC CGGTTTCTGC TTCAGCAACG CTCAGGACGG CACGTTCTTC 
AACGGCAGCG GATACGCAGC TTTCATGAAA GAGGGCTATA ATGTGGGCTC AGACGTGACG 
GTGAGTCTGG AGTTTCGCTC CACGGCGCCG GATGGTGTTT TATTAGGAAT CAGCAGCACT 
AAAGTGGACG CCATCGGCCT GGAGCTGCTC AACGGACAGG TGGTGTTTAA TGTGAATAAC 
GGTGCGGGCC GCATCTCGGT GAGCAGCAGG AGCAGCGTGT CCATGTGTGA CGGACGCTGG 
CGCACTCTAG TGGCCAAAAA GCAGAAGCAC AGCTTGAGTT TAACGGTGGA CGGCGTGACG 
GTGAACGCAG AAAACCCGCA CTCCTCCTCC ACATCCGCAG AGACCAAAAA CCCCATATAT 
GTGGGAGGAC ATCCAGCGGA TGTAAAGCAG AATTGTCTGA GCGTCAGGCA GTCGTTTCGT 
GGCTGTATGA GGAATCTGCG AGTGTTTAAG GGTCACGTCA CCGATGTGCT GGACTTCAGC 
TCTGCATTCA CACTCCTGCA CGTTTCCCCT CACTCCTGCC CTGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001030158.1
CDS	129..9356
Translation	MMEMRMKMLWLLALVFSSVICVEAQQRGLFPAILNLASNAEISTNATCGDPDPEMFCKLVEHVPGRRIRNPQCRICDANSQNPKEQHPITNAIDGTNLWWQSPSIKNGRQFHWVTVTLDLRQVFQVAYIIIKAANSPRPGNWILERSLDGVNFQPWQFYAISDTECLTRYNITPRIGPPTYKRDDEVICTSYYSRLVPLEHGEIHTSLINGRPSADDLTPELLEFTSARFIRLRLQRIRTLNADLMTLSYRDPKDVDPIVTRRYYYSIKDISVGGMCICYGHAQSCPWDPVTKKLQCVCEHNTCGESCNECCPGYHQEPWQPGTLSDGNTCEKCNCHNKADDCFYNQTVADLKLSMNTHGQFTGGGVCVNCRHNTAGVNCETCADGYYRPHQVSPYAEEPCVECQCDMRGSVSPVCIRDDNHANPDAGLSPGQCVCKEGFAGEKCDRCAFGFRDFPVCSRCECNLDGSHNTDPCMECVCKANVMGSHCDLCKQGFYNLQASNPEGCTECFCFGVSDVCESSTWFSSSVVHRDGVLHRLHQTSISFWSPVSDENLIISNRSTDADPSSVWTWAAPEPFLNNKLTSYGSFLNYSVAYDTSEENVDKTLRSHFSVIIEGNGRTLRQAQSARLLLNAHTHQRVFVQMLPQIFIDSHSGRSVQRDELMTVLADVAALRVRAELEDSAEGTLRLSHVSLGVGDSNSDVSKISLDVEHCECPWGYSGTSCELCIPGFYRVGGILFGGNCLPCECNDHATECDINGECLGCAHNTTGPHCDQCLPGFYGDASEGTPDDCQRCSCPLTLASNNFSPTCQLQAPGEFICDQCQSGYTGDKCQRCADGYFGEPVVPGQRCSPCECNGNVDPSEAGRCDTHTGECLKCVGHTAGPHCERCRDGYYGDAIQEKNCQACGCYSNGSLSTICNLITGQCECKHNVVGKTCDRCQEGYHGINSGEGCRPCECNQSGALSASCDEEGRCQCITGVTGDKCDRCHHGYYNFKENGCTACDCAHTHGNCNGQTGECICPPHTHGLKCEQCDEGHWGHDGVSGCKVCNCSVVGSSSSQCDLSSGQCVCALQFSGLTCDRCALGYRNFPQCSACDCNPNGTRAQFCDEALGVCGCEDHGQCSCKDNVGGRGCDECKSGSFGLSALNPAGCSPCFCFGLSDVCEELSGLVRAPIVLGSSPALLRVVSQSDLQGTLEGVYYSDDEMLLDVDQLNKLSLLTGPYYWRLPQKYNGNKLLSYGGRLSYTLTFFAQDGVGLANQEPQVLMRGGHLRKLVIYTDASAPANGIRSTQEHALTEHKWKYFNSVSDEAVSHADFMSVLSNLEYVIIKASYGSGLQQSRISNISMETALEADELPEGGDVARLVEICECPPGYAGLSCQECAPGYYRQAVSELNMKGRNRPLIQPCVPCRCSNHSQSCDLHTGQCLGCQHNTAGEHCHVCAAGYYGKVQGSVSDCSLCACPLRGQSFSSTCVLEGAGDFRCDRCEEGYEGRYCERCAVGYFGNPSEPGGRCQVCACSESGSVHSVCDAHTGRCECKPGVRGHLCDQCEERHVLVHEQCVSCNDSCTGVLLDDLDALDVSITSVNLTGVILAPYSQLMTIQNQTRELTSLMSWNQTPDYQLTAGEEHSRNLSGQITDLQQQVQSVFEDSGDVLLTSEQRFTQGKQLLELINNIHSAIHGLVEQVSSVNVTLDEGLDEANSTFLLEETSETLEKIRSFNLTHCRNAAEIQLSSAVSVLEKVEDEISKPYRNNQIRRENISTTLNTHNLSLQQVQESLYTAKTHNNQSRRLLQDIQSNTHTLTISRNNVSSLNHELETQVQEAQDLLTDALNIAEDMDNGATTLDELKNDLELWSPSLRKHVDSLVMDLTKRDALQLVYRAEDHAQALSKQAQALNSSLSEVSSASVNISIATRTNANIRENIQLAADLALAANQNASSALNMTSLTEQEKRRLQKSSEILDRAALLKNHTDGLMQNVSAVTSRLSLVAANIQNFTQMLPEALGILRNLPEGSRAEILQVKQQTEAANASLQEALERLRDYRLKLDESSSALGSADNSTQRTNQLLRDSQDTANAAGSRLSEADVKADRLLERLKPLQTLGETLSRNLSDIRELISQARKQAASIKVAVSADRNCVRAYRPEVTSSNFNTLTLTMKTSEPDNLLFYMGSSSSEDFMALEMHDGKVSFLWDAGSGHNRLEYPDVQINNDKWHRINATRFGRHATLTVQQTDSEPLPAVKSSAAGSATVMDVNKHTWVYVGGLGGQVKKSPAVKITHFRGCMGEALLNENNIGLWNYAERQGECGGCFRSPRTEDTSFHFDGSGFSVVEKSLRSMSTSVVMFFKTLSPNGLLLYLASNGTRDFLSIELVEGKVHLTFELGSGALTMTSSRTYNTGSWYRIALQRNKRKGHLSVMAASNPSEREVLEAEAPGQASDLNRSDLDPIYIGGLPASRPIRRQVVARSFVGCMKNVEIARTNFDLLREAYGVKKGCVLKPIRSVSVFGGGFLEMPAVLLSSHTEIMSTFSSRRDSGVILAGFSRSSRHTQQPLLLLLLVSGGLELRVSVSDGAAVQKAELRGSFSDGHEHSLILQRNKRTITVVVDEGIQASLKLPASAEKSLTLERLYIGGVPPAEGDTHTLTPAFYGCIRNTAVDGMLLDLSAALRYEQVDMDSCLLEPRPRRVLLPDDPEPTSDPDTRPLTPAAAELSALTPNSGSCASADHTQTIPESHQFGLSRHSHLIMGFKNRTVRTSFAVKLSVRTFAHSGVLFYMANTNQQDYAVLQLQAGRLHLSCDLGKGAASATLKNTISDGRWHTVKAEFSKKTVVVSVDGIESDHISTKGHTLDVEGKLYVGGLPPTYTAKRIGNVTHSVAACVQDLTFNGVPMNLNNPLSSHATGFCFSNAQDGTFFNGSGYAAFMKEGYNVGSDVTVSLEFRSTAPDGVLLGISSTKVDAIGLELLNGQVVFNVNNGAGRISVSSRSSVSMCDGRWRTLVAKKQKHSLSLTVDGVTVNAENPHSSSTSAETKNPIYVGGHPADVKQNCLSVRQSFRGCMRNLRVFKGHVTDVLDFSSAFTLLHVSPHSCPA

Target ORF information:

RefSeq Version	NM_001034986.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio laminin, alpha 1 (lama1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001034986.1

ORF Insert Sequence:

ATGATGGAGA TGAGGATGAA GATGCTGTGG CTGTTAGCGC TGGTTTTCTC CTCAGTGATT 
TGTGTGGAGG CTCAGCAGAG AGGTTTGTTT CCTGCAATCT TGAATCTCGC CAGCAATGCC 
GAAATCAGCA CCAACGCCAC CTGTGGCGAT CCCGATCCCG AGATGTTCTG CAAACTGGTG 
GAGCACGTTC CTGGAAGACG GATCCGCAAC CCGCAGTGTC GCATCTGTGA CGCAAACAGC 
CAGAATCCCA AAGAGCAACA TCCAATTACT AACGCCATCG ATGGAACCAA CCTGTGGTGG 
CAGAGCCCGA GCATCAAGAA CGGCCGTCAG TTCCACTGGG TCACAGTCAC GCTGGACCTG 
CGACAGGTGT TCCAGGTGGC GTACATCATC ATTAAAGCGG CGAACTCTCC TCGACCTGGA 
AACTGGATTC TGGAGCGTTC CCTGGACGGC GTGAACTTCC AGCCGTGGCA GTTTTACGCC 
ATTAGCGATA CTGAGTGTTT GACGCGCTAC AACATCACAC CGCGCATCGG CCCGCCGACA 
TACAAGAGAG ACGACGAGGT GATCTGCACA TCGTACTACT CTCGCCTGGT GCCGCTGGAG 
CATGGAGAGA TCCACACGTC TCTGATTAAC GGCCGTCCCA GCGCTGACGA TCTGACACCG 
GAGCTGCTGG AGTTCACATC GGCGCGGTTT ATACGCCTGC GCCTGCAGAG GATTCGGACG 
CTCAACGCAG ACCTGATGAC CCTCAGCTAC CGAGACCCCA AAGACGTGGA CCCCATCGTC 
ACCCGACGGT ATTATTACTC CATCAAGGAC ATCTCAGTGG GCGGGATGTG CATCTGCTAT 
GGCCACGCCC AGAGCTGCCC CTGGGACCCT GTTACCAAGA AGCTGCAGTG TGTGTGTGAA 
CACAACACTT GTGGTGAGAG CTGTAATGAG TGTTGTCCTG GATACCATCA GGAGCCATGG 
CAGCCGGGAA CGCTCTCCGA CGGCAACACT TGCGAGAAGT GTAATTGTCA CAACAAAGCC 
GACGACTGTT TCTACAACCA GACGGTGGCG GATCTGAAGC TGAGCATGAA CACACACGGC 
CAGTTCACAG GAGGAGGAGT GTGTGTGAAC TGCAGACACA ATACAGCGGG AGTGAACTGT 
GAGACCTGTG CGGACGGATA CTACAGACCC CACCAGGTGT CTCCGTATGC AGAAGAGCCG 
TGTGTGGAGT GTCAGTGTGA TATGAGGGGT TCAGTGAGTC CTGTGTGCAT CAGAGATGAT 
AATCACGCAA ACCCAGACGC AGGTCTGTCT CCAGGTCAGT GTGTGTGTAA GGAGGGTTTT 
GCGGGAGAGA AGTGTGACCG ATGTGCGTTC GGCTTCAGAG ATTTCCCGGT GTGTTCTCGC 
TGCGAGTGTA ACCTGGATGG GAGCCACAAC ACCGACCCCT GTATGGAGTG TGTGTGCAAG 
GCGAATGTGA TGGGCTCTCA CTGTGACCTG TGTAAGCAGG GCTTCTATAA CCTGCAGGCC 
AGCAATCCTG AAGGCTGCAC CGAGTGTTTC TGCTTCGGTG TGTCAGATGT GTGTGAGAGC 
TCCACCTGGT TCAGCAGCTC GGTGGTGCAC AGAGACGGCG TCCTCCATCG CCTGCACCAG 
ACCTCCATCA GCTTCTGGTC TCCAGTTTCT GATGAAAACC TGATCATCTC CAACAGATCC 
ACAGATGCAG ACCCCTCCTC TGTGTGGACA TGGGCCGCTC CGGAGCCTTT CCTCAATAAC 
AAGCTGACGT CATATGGAAG TTTCTTGAAC TACAGTGTGG CGTATGACAC TTCTGAGGAG 
AACGTGGACA AAACACTGCG CTCACACTTC AGCGTAATTA TTGAGGGTAA CGGGCGCACG 
CTGAGGCAGG CGCAGTCGGC TCGTCTGCTG CTGAACGCTC ACACACACCA GCGTGTGTTT 
GTGCAGATGC TTCCGCAGAT CTTCATAGAC TCACACAGCG GCAGGAGCGT GCAGAGAGAC 
GAGCTGATGA CGGTGCTGGC GGACGTTGCT GCGCTCAGGG TTCGAGCCGA GCTGGAGGAC 
AGCGCTGAGG GAACGCTCAG GTTGAGTCAC GTGTCTTTAG GTGTTGGAGA CTCAAACTCC 
GATGTGTCTA AAATCTCTCT AGATGTGGAG CACTGTGAGT GTCCGTGGGG ATATTCAGGC 
ACTTCCTGTG AGTTGTGTAT TCCTGGCTTT TATCGGGTCG GTGGGATTTT GTTCGGAGGA 
AACTGTCTTC CGTGCGAGTG TAACGATCAC GCGACGGAGT GTGACATCAA CGGAGAGTGT 
CTGGGCTGTG CGCACAACAC CACGGGGCCT CACTGTGATC AGTGCCTGCC GGGTTTCTAT 
GGTGACGCGA GCGAGGGGAC GCCGGACGAC TGCCAGAGAT GCTCCTGCCC GCTGACACTG 
GCGTCAAACA ATTTCAGCCC CACGTGTCAG CTGCAGGCTC CCGGTGAATT CATCTGTGAT 
CAGTGCCAGT CTGGATACAC CGGAGACAAG TGCCAGAGGT GTGCTGATGG GTATTTTGGT 
GAGCCGGTGG TCCCTGGTCA GCGCTGCTCC CCCTGTGAGT GTAACGGGAA TGTGGACCCA 
TCTGAAGCGG GACGCTGCGA CACCCACACT GGGGAGTGTC TGAAGTGTGT AGGGCATACG 
GCCGGGCCAC ACTGCGAACG CTGCCGGGAC GGATATTATG GAGACGCCAT CCAAGAGAAG 
AACTGCCAGG CCTGTGGTTG TTATAGTAAC GGCTCTCTGT CCACCATCTG TAACCTGATC 
ACGGGTCAGT GTGAATGTAA ACACAATGTG GTCGGAAAAA CCTGCGACCG CTGTCAGGAG 
GGTTATCACG GTATTAACAG TGGTGAAGGC TGCAGACCGT GTGAATGTAA CCAATCAGGC 
GCTCTGTCTG CGTCATGTGA TGAGGAGGGG CGGTGCCAGT GCATAACTGG GGTAACGGGT 
GACAAATGTG ATCGATGTCA CCATGGATAC TACAACTTCA AAGAGAACGG ATGCACAGCA 
TGCGACTGCG CACACACTCA TGGAAACTGC AACGGTCAGA CGGGCGAGTG TATCTGTCCT 
CCACACACAC ACGGGCTGAA GTGTGAACAG TGTGATGAAG GTCACTGGGG TCACGACGGA 
GTGTCCGGCT GTAAGGTGTG TAACTGCAGT GTTGTGGGCT CCAGCTCGTC TCAGTGTGAT 
CTGTCCTCGG GTCAGTGTGT GTGTGCGCTG CAGTTCTCCG GCCTCACATG CGACCGATGC 
GCTTTAGGCT ACAGAAACTT CCCACAATGC TCTGCCTGCG ACTGCAACCC GAACGGCACG 
CGTGCGCAGT TCTGTGACGA GGCACTAGGA GTGTGTGGCT GCGAGGATCA CGGGCAGTGT 
TCCTGCAAGG ATAATGTGGG CGGCAGAGGC TGTGACGAGT GTAAGAGCGG CTCCTTCGGG 
CTCTCGGCCC TCAATCCGGC CGGCTGCAGC CCCTGCTTCT GCTTTGGCCT TTCAGACGTG 
TGTGAGGAGC TGAGCGGATT GGTGCGCGCG CCGATTGTTC TGGGTTCATC TCCGGCTCTG 
CTGCGTGTAG TGTCTCAGAG TGACCTGCAG GGGACGCTGG AGGGCGTTTA TTACAGCGAT 
GACGAGATGC TGCTGGACGT GGATCAGCTC AACAAACTCT CTCTCCTCAC CGGACCGTAT 
TACTGGAGAC TCCCACAGAA ATACAACGGC AACAAGCTGC TGTCGTATGG AGGAAGACTC 
TCCTACACCC TCACCTTCTT CGCTCAGGAC GGCGTGGGAT TGGCCAATCA GGAGCCTCAG 
GTGCTGATGA GAGGAGGACA CCTGCGTAAG CTGGTCATCT ACACTGATGC TAGCGCTCCT 
GCTAACGGCA TCCGCAGCAC ACAGGAGCAC GCTCTCACCG AGCACAAGTG GAAATACTTC 
AACTCAGTGT CTGATGAAGC CGTGAGCCAC GCTGACTTCA TGTCTGTGCT GAGTAACCTG 
GAGTACGTCA TCATTAAAGC CTCGTACGGG TCCGGACTGC AGCAGAGCAG GATCTCCAAC 
ATCTCCATGG AAACTGCATT GGAGGCAGAT GAGCTTCCTG AAGGTGGAGA TGTGGCGCGG 
TTGGTGGAGA TTTGTGAGTG TCCGCCGGGA TACGCTGGAC TGTCCTGCCA GGAGTGTGCT 
CCAGGATATT ACCGGCAGGC TGTATCTGAG CTCAACATGA AGGGCAGAAA TCGGCCGCTC 
ATTCAGCCCT GTGTGCCCTG CAGATGCAGC AACCACAGCC AGTCATGTGA CCTGCACACC 
GGCCAATGCC TGGGCTGTCA GCACAACACC GCTGGAGAAC ACTGCCATGT TTGCGCTGCA 
GGGTATTATG GGAAGGTGCA GGGGTCGGTC AGTGACTGCT CTCTGTGTGC CTGCCCACTG 
CGGGGTCAAA GTTTCAGCTC AACATGTGTG TTAGAAGGTG CTGGAGACTT CCGCTGCGAC 
CGCTGTGAGG AGGGATATGA GGGACGATAC TGTGAGAGGT GTGCAGTGGG GTATTTCGGG 
AACCCGTCGG AGCCGGGTGG CCGCTGTCAG GTGTGTGCGT GCAGTGAGTC GGGCTCAGTG 
CACAGTGTGT GTGACGCACA CACAGGCCGC TGCGAGTGTA AACCTGGGGT CAGAGGTCAC 
CTGTGTGACC AGTGTGAGGA GAGGCATGTG CTGGTCCACG AGCAGTGTGT GTCATGTAAT 
GACTCCTGCA CTGGTGTTTT GCTGGATGAT CTGGATGCTT TAGACGTGTC CATCACATCT 
GTGAACCTGA CGGGTGTCAT TCTGGCCCCG TACAGCCAGC TGATGACCAT CCAGAACCAG 
ACCCGAGAGC TCACGAGCCT GATGTCGTGG AACCAAACTC CAGATTATCA GCTGACCGCT 
GGAGAAGAAC ACTCGAGGAA CCTGAGCGGT CAGATCACTG ACCTTCAGCA GCAGGTCCAG 
AGTGTGTTTG AAGACAGTGG TGATGTGCTG CTGACCTCAG AGCAGAGATT TACTCAAGGG 
AAACAACTGC TGGAGCTCAT CAACAACATT CACTCTGCCA TCCACGGTCT GGTGGAGCAG 
GTGAGTTCAG TGAACGTGAC TCTGGATGAA GGTCTGGATG AAGCCAACAG CACATTCCTG 
CTGGAGGAGA CCAGCGAAAC CCTGGAGAAA ATCAGGAGCT TTAATCTGAC GCACTGCAGG 
AACGCTGCGG AGATCCAGCT CAGCTCTGCA GTGTCTGTGC TGGAAAAGGT CGAGGATGAG 
ATTTCAAAGC CTTACAGAAA TAACCAGATC AGACGGGAAA ACATCTCCAC AACACTCAAC 
ACACACAACC TGAGTCTCCA GCAGGTCCAG GAGAGCCTTT ACACGGCTAA AACGCACAAC 
AACCAGAGCC GACGCCTGCT CCAGGACATC CAGAGCAACA CACACACACT CACCATCTCC 
AGGAACAATG TCAGCAGTCT GAACCATGAA CTAGAAACAC AAGTACAGGA AGCTCAAGAC 
CTGCTGACCG ACGCCCTCAA TATTGCAGAA GACATGGACA ACGGGGCGAC AACTCTGGAT 
GAGCTGAAGA ATGATCTGGA ATTGTGGAGT CCGTCTCTCA GGAAGCATGT GGACTCTTTA 
GTCATGGATT TGACCAAAAG AGATGCTCTT CAGCTGGTTT ACAGGGCTGA GGATCACGCA 
CAAGCTCTCA GCAAACAAGC ACAAGCCCTG AACAGTTCTC TGTCTGAGGT CAGCTCTGCA 
TCAGTTAACA TCTCCATCGC CACCCGAACC AATGCCAACA TCAGAGAAAA CATACAGCTG 
GCGGCAGATC TGGCCCTCGC AGCCAATCAG AACGCAAGCT CCGCCCTCAA TATGACGTCA 
CTGACGGAGC AGGAGAAGAG GAGGCTGCAG AAGAGCTCAG AGATTCTGGA CAGAGCTGCA 
CTTCTGAAAA ACCACACCGA CGGCCTCATG CAGAATGTCA GTGCAGTGAC CAGCAGGTTG 
AGTCTGGTTG CAGCAAACAT CCAGAACTTC ACACAGATGC TCCCAGAAGC CCTCGGCATC 
CTCAGAAACC TTCCTGAAGG CAGCAGAGCT GAAATCCTGC AGGTGAAGCA GCAGACAGAA 
GCAGCGAATG CGTCTTTACA GGAAGCTCTG GAGCGTTTGC GGGATTATCG CCTAAAGCTG 
GACGAGTCTT CATCAGCTCT GGGGTCAGCC GATAACAGCA CACAGAGGAC CAATCAGCTG 
CTCAGAGACT CACAGGACAC CGCAAACGCT GCTGGATCCC GCCTGAGCGA GGCTGATGTG 
AAAGCTGATC GTCTGCTGGA GCGTCTGAAG CCTCTGCAGA CTCTGGGAGA AACGCTGAGC 
AGAAACCTGT CCGACATCAG AGAACTCATC AGTCAGGCGC GCAAACAGGC TGCATCGATT 
AAAGTGGCCG TCTCTGCAGA CCGCAACTGT GTACGCGCGT ACAGACCTGA GGTGACGTCC 
AGCAACTTCA ACACACTCAC ACTGACCATG AAGACCAGTG AGCCCGACAA CCTGCTCTTC 
TACATGGGCA GCAGCTCCAG CGAGGATTTC ATGGCTCTAG AAATGCATGA TGGGAAGGTG 
TCCTTCCTGT GGGACGCCGG TTCAGGACAC AACAGACTGG AGTATCCGGA CGTTCAGATC 
AACAATGATA AATGGCACAG GATCAATGCC ACGCGGTTTG GGAGACACGC TACACTGACG 
GTTCAGCAGA CGGACTCGGA GCCGCTGCCC GCCGTAAAGA GCAGCGCCGC TGGATCTGCC 
ACTGTGATGG ACGTCAATAA ACACACATGG GTGTATGTGG GCGGCCTGGG TGGACAGGTG 
AAGAAATCTC CAGCAGTGAA AATAACCCAT TTCAGAGGCT GTATGGGTGA AGCGTTGCTC 
AACGAGAACA ACATCGGCTT GTGGAATTAT GCTGAGAGAC AGGGCGAGTG CGGAGGCTGC 
TTCAGGAGTC CTCGCACTGA AGACACCTCG TTTCACTTCG ACGGCAGCGG CTTCTCTGTG 
GTGGAGAAAT CTCTGCGCTC CATGTCCACC AGCGTGGTGA TGTTCTTCAA GACTCTGTCC 
CCCAACGGCC TGCTGCTGTA TCTGGCCTCC AACGGCACGA GAGACTTTCT GTCCATCGAG 
CTGGTGGAGG GAAAGGTTCA TCTGACCTTT GAACTGGGCT CCGGAGCGCT GACCATGACC 
TCCAGCAGAA CATACAACAC CGGATCCTGG TACCGAATCG CCCTGCAGAG GAACAAGCGC 
AAAGGTCATT TATCTGTCAT GGCTGCTTCA AACCCGTCCG AGCGTGAGGT GCTGGAGGCG 
GAGGCTCCGG GTCAGGCTTC TGACCTCAAC CGATCCGACC TTGACCCCAT TTACATCGGT 
GGATTGCCAG CCTCTCGACC AATCAGGAGG CAGGTTGTCG CTCGCTCTTT CGTCGGCTGC 
ATGAAGAACG TGGAAATCGC ACGAACAAAC TTCGACCTGC TCAGAGAGGC GTACGGCGTG 
AAGAAAGGCT GCGTCCTCAA GCCCATTCGC AGTGTGTCAG TGTTTGGCGG AGGGTTTCTG 
GAGATGCCGG CGGTGCTGCT TTCCTCTCAC ACTGAAATCA TGAGCACCTT CTCCAGCAGA 
CGTGACAGCG GAGTCATCCT GGCCGGATTC AGCCGCTCAT CCAGACACAC ACAACAGCCG 
CTGTTACTGC TGCTGCTGGT GTCTGGAGGT CTGGAGCTGC GTGTGAGTGT GTCTGATGGA 
GCAGCGGTGC AGAAAGCAGA ACTCAGAGGA TCCTTCAGTG ACGGACATGA ACACTCACTC 
ATCCTGCAGA GGAACAAACG GACGATCACA GTGGTTGTGG ATGAGGGCAT CCAGGCCTCG 
CTGAAGCTGC CGGCGTCTGC AGAGAAAAGC CTGACTCTGG AGCGGCTCTA CATCGGCGGC 
GTTCCTCCAG CAGAGGGAGA CACACACACA CTCACACCTG CCTTCTACGG CTGCATTAGG 
AACACTGCGG TCGACGGCAT GTTGTTGGAT CTGTCCGCTG CGCTGCGCTA CGAGCAGGTG 
GATATGGACA GCTGTTTGCT GGAGCCGCGG CCCAGACGAG TACTGCTGCC TGATGACCCG 
GAGCCGACAT CTGACCCCGA CACACGACCT CTGACCCCTG CAGCAGCGGA GCTGAGTGCG 
CTCACACCAA ACAGCGGCAG CTGTGCATCA GCGGACCACA CACAGACCAT CCCAGAATCC 
CACCAGTTCG GCCTGTCTCG ACACAGTCAC CTAATCATGG GTTTCAAAAA CCGGACGGTC 
AGGACCAGTT TTGCAGTGAA GCTGTCGGTG CGCACGTTTG CTCACAGCGG CGTTCTGTTC 
TACATGGCGA ACACAAACCA GCAGGATTAC GCAGTTCTGC AGCTGCAGGC CGGACGACTG 
CATCTGTCCT GTGACCTGGG CAAAGGAGCG GCCAGCGCCA CGCTCAAAAA CACCATCAGT 
GACGGACGCT GGCACACGGT GAAGGCCGAG TTCAGTAAGA AGACTGTTGT GGTCAGTGTG 
GACGGGATTG AATCTGACCA CATCTCCACT AAAGGACACA CACTAGACGT CGAGGGGAAG 
TTGTACGTCG GTGGACTTCC TCCCACATAC ACCGCCAAGA GAATCGGAAA CGTGACCCAC 
AGTGTGGCGG CGTGTGTGCA GGACCTGACG TTTAACGGTG TCCCAATGAA CCTGAATAAT 
CCGCTGTCAT CTCACGCCAC CGGTTTCTGC TTCAGCAACG CTCAGGACGG CACGTTCTTC 
AACGGCAGCG GATACGCAGC TTTCATGAAA GAGGGCTATA ATGTGGGCTC AGACGTGACG 
GTGAGTCTGG AGTTTCGCTC CACGGCGCCG GATGGTGTTT TATTAGGAAT CAGCAGCACT 
AAAGTGGACG CCATCGGCCT GGAGCTGCTC AACGGACAGG TGGTGTTTAA TGTGAATAAC 
GGTGCGGGCC GCATCTCGGT GAGCAGCAGG AGCAGCGTGT CCATGTGTGA CGGACGCTGG 
CGCACTCTAG TGGCCAAAAA GCAGAAGCAC AGCTTGAGTT TAACGGTGGA CGGCGTGACG 
GTGAACGCAG AAAACCCGCA CTCCTCCTCC ACATCCGCAG AGACCAAAAA CCCCATATAT 
GTGGGAGGAC ATCCAGCGGA TGTAAAGCAG AATTGTCTGA GCGTCAGGCA GTCGTTTCGT 
GGCTGTATGA GGAATCTGCG AGTGTTTAAG GGTCACGTCA CCGATGTGCT GGACTTCAGC 
TCTGCATTCA CACTCCTGCA CGTTTCCCCT CACTCCTGCC CTGCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hedgehog signalling acts upstream of Laminin alpha1 transcription in the zebrafish paraxial mesoderm.
Matrix biology : journal of the International Society for Matrix Biology6258-74(2017 Oct)
Pickering J,Cunliffe VT,Van Eeden F,Borycki AG

NgAgo-based fabp11a gene knockdown causes eye developmental defects in zebrafish.
Cell research26(12)1349-1352(2016 Dec)
Qi J,Dong Z,Shi Y,Wang X,Qin Y,Wang Y,Liu D

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lama1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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