EPG5 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	EPG5
Entrez Gene ID	569543
Full Name	ectopic P-granules autophagy protein 5 homolog (C. elegans)
Synonyms	si:dkey-165i4.3
General protein information	Preferred Names ectopic P-granules autophagy protein 5 homolog (C. elegans) Names ectopic P granules protein 5 homolog
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001110525.1	NP_001103995.1	ectopic P granules protein 5 homolog
XM_009301680.3	XP_009299955.1	ectopic P granules protein 5 homolog isoform X2
XM_005165361.4	XP_005165418.1	ectopic P granules protein 5 homolog isoform X1
XM_005165361.3	XP_005165418.1	ectopic P granules protein 5 homolog isoform X1
XM_009301680.2	XP_009299955.1	ectopic P granules protein 5 homolog isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EPG5	XP_512111.2
Canis lupus familiaris (dog)	EPG5	XP_005622908.1
Mus musculus (house mouse)	Epg5	NP_001182562.1
Danio rerio (zebrafish)	si:dkey-165i4.3	NP_001103995.1
Caenorhabditis elegans (roundworm)	epg-5	NP_495343.1
Homo sapiens (human)	EPG5	NP_066015.2
	LOC700766	XP_001089105.1
Rattus norvegicus (Norway rat)	Epg5	XP_006255041.1
Gallus gallus (chicken)	EPG5	XP_004949102.1
Drosophila melanogaster (fruit fly)	CG14299	NP_001247184.1
Xenopus tropicalis (tropical clawed frog)	epg5	XP_004911392.1
Bos taurus (cattle)	EPG5	XP_003587838.1

Gene Ontology
Bibliography

Related articles in PubMed

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Gaudet P, Livstone MS, Lewis SE, Thomas PD
Briefings in bioinformatics12(5)449-62(2011 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EPG5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EPG5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa35216	NM_001110525.1 Latest version!	Danio rerio ectopic P-granules autophagy protein 5 homolog (C. elegans) (epg5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa10746	XM_005165361.4 Latest version!	Danio rerio ectopic P-granules autophagy protein 5 homolog (C. elegans) (epg5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa45788	XM_009301680.3 Latest version!	Danio rerio ectopic P-granules autophagy protein 5 homolog (C. elegans) (epg5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
View Old Accession Versions >>
ODa10746	XM_005165361.3	Danio rerio ectopic P-granules autophagy protein 5 homolog (C. elegans) (epg5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa45788	XM_009301680.2	Danio rerio ectopic P-granules autophagy protein 5 homolog (C. elegans) (epg5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EPG5 ( NM_001110525.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001103995.1 Danio rerio ectopic P-granules autophagy protein 5 homolog (C. elegans) (epg5), mRNA.

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CloneID	ODa35216
Clone ID Related Accession (Same CDS sequence)	NM_001110525.1
Accession Version	NM_001110525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Danio rerio(zebrafish)
Product	ectopic P granules protein 5 homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR753867.11. On Nov 16, 2007 this sequence version replaced XM_692939.2. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCTTCATT TACAGGTCAA AAAGGAGAGG CAGTCAGAGA GCGTCAGACA GCCCTCGGGA 
TCTTCTAAAG CAGTTAATGA GCCTGAGTTC ACTGATGTGC CGCTCAGCCT CCCTCATGTG 
CCTCCTGACC TCAACACAGC TGCCTCTCAG ACTTCACTAG ATCATCATGA CAAAGCTGAA 
AATGAGCAAC AGCATGTTCT CTTATGCGAG AAGCCTGAAG TAGTACAGAA ATCGACGTGT 
CATGAAGACA AAGAGCATGA ATTGCCATCA CAAGATGCTG CACCTCGGCT GGACCAAACA 
TCAGTGCTTG CTCCGTCCAG CCAAAGTGAT CCCAAACCCC TCAGTGCTAA ACTTGAGCCA 
TTTAGTGTCC CCTCTATATA TCCCTCTATC CCAGCTTCCC TCTCCCAGCC TCAGCTTTCA 
GAGGACTCGT ACTCTATCTC CTACGAACTG TTAGATGGCT CTTTAATGCC AGCAGCGTCT 
GAGAGACTAG TAGCTCCGGC TGTTTTGACT CTGGCTAACC AGGAGTCCTC TCCGCCCAGC 
TTGGTTCCAG TGGAGGTTGT GGATGTGGAA AGATCTAGAG AGAGGCTTTA CCCAGAGCTG 
CCTAAGACCT GCCAGTCTGT GAAGCCCTTC ACTAGTGAGC AGCTGCGCAT GTGGGAGCCC 
GGTTCCTGGC TGGAAAACGT GGAGCTGCAT GAAGCCGAAT TCCAGAATCT GGCTCATCAA 
GAGGGACATG AGCTGTACGA GCTGCTGCTG AGCTACTGGA GGTGCCGGAA GCAGCTGACG 
CAGGCCCAGA CTGAACTGCA GGCTGCCAAC TCGGACTGCA AAAATGTGCA GAATCGCCTG 
TGGAGCTTCA AGGATGAAAG GCTTTCGTTA CAGGGTATAT GTGCAGACCA GAATAAGGTG 
TATGGCTACC ACTGCTACCA GCAGGTGACA CTGAACGAGG CTGCGTTAGC TGAGCTGAAG 
CACCTGTTTT CTGCTAAAGC GGAAATCCTC CATCAGACAG TGGCTCTGCA CTCATACACC 
TCCATCCTGT CCCGTCTGCA AGTGGAGTCT TATCTCTACC GCTTGCTCAG CAGTAACCCC 
ACCACCAAGA GTGTGGCTGT GCAAGATACA AGTGCAGGCA ATCCAAAGTC TCAGCCCTCG 
TCTTTGCAGC CGCTGAAGGA AAGCATCAGT GTGCTGTTCA TCTTCACCCG ACGGGTCCTA 
GATGATTCTC AGTTTCAGGC TGACATCCAT CTTTGGCTTC AGAGGCTGGT GTCTGTGCTG 
CATCAGGTCG GTTCTACAGG AGATCATCTA TTCATCCTAA ACCACCTGCT CTGCTGCCCG 
GCAGGTGTAG GGAAATGGGC TGTGCCATTC CTACAGATCA AAGTATTGGA TAACCCATCT 
GGAGTATATC ATTTCATGCA GGCCCTTGCT ATCCTTATGT CTCCAGCAAG GCACCGTCCA 
GACTTCCTGG GACACATGAA GCCCAGTGAT GCAAGGGGCA ACAACAGCCT AACAGAACCT 
GCATCTGGTA ACTGGACTCT AGTGGATGAA GGTGGAGAAG AGGACGAAGA CCCAGAAACG 
AGCTGGATGC TGCTGTCTGA GGATGATCTG GTTTCTCTGT TCTCTCAGTT TCCCTTTGAT 
GAGCTCTTCA AACATTTACT GGGCATCTGC TCTAAAGGGG ACTATCAGCC TCTAGCCACC 
ACAAGTCAAA AGATGATGAA GATATTTGCG TTTGCCTCTT CACTGGTTGA GTTGCTAGCA 
GTCGGCTTGC AGACCTACAA CAGAGCACGC TATCGGCAGT TTGTGAAGAG GATTGGTCAC 
ATGATTCGGA TGACGCTCTG CTATGTGAGT GACCACTGGG CGCAGTACAT CAGTCTGACC 
GGTGTGAGCA GGACCACAGT GCAGGAGCAG TCATTTTCAA TGGAGAAACT GCAGGTGGAG 
TTTGACCATC TCTTCCTGAG GGCGGTGCTG CATGTGCTTA AGGCCAAGAG ACTGGGCATA 
TGGCTGTTCA TGTCTGAGAT GCCGTATGGG ACATTGTCCA GCTCCATGCT GTGGAAGATC 
TTCTACATTA TGCAGTGTGC AGAGACAGAT GGTCTGGAGA AGCTCAGTTG TAGCTTGAGT 
GCTGAGGACT GTATCCAGAG GCTCAGAGAA TCAAGCCATC AGGAGAGGTT TCAGCTTTGG 
CTGTCTGAGA TAAACAGCTC AGATGGGATC TGCCTGTTGA CAACGTTTGC GCACATGGCC 
CAGCCGAGAC GCACCGATGT AGACCCTGAG TTTGTCAGGA ACATAGTGCT AGAGATATAC 
GAGGTTTCCT ATGTAAGTGT CACCACCCGT GAGATCTTCT CTAAAGTTGG ACGGGAATTG 
CTTGCAGCGA TAGCGGCTGC CCACCCTCAC ATCATCTCTG TGTTGCTGGA GAGACTGAGA 
GAGACCATAG AGAGAGTGGG CATGGTGTCG TTGTACCTGT TTAAAGAGTT GCCCTTGCAC 
TTGTGGATGC CAGCTCAGGA GGAGGTGGGG CTGATCAGAG ACTGGCTGAT GAACTTCAGT 
CTCACTGCTG TTGAGAACAG ATTAGCCTGC ATTATACTAG AGGGCCTCAA CTGGGGATTT 
CAGCCAAATG GTTGCCTTAT TTTACCTGCA CCTCTGCACA GTGACGTTGC CTTGTTGGTG 
ATTGAGGCAT ATCAAAAATA TCTTACAGAT AAACCATATG GTGGCATCCT CTCAGAAGGC 
ATTAAACAGG TGTCTTACCT GGCTAACGTG GTGAGGCTCG GCCAGACTCC AGAATCTTCC 
TTTAATCAGT GGGCTTGGGA TATAGTTCTG AGGCTAAAGC TCCACGCCAA TGAAAGAAGC 
CCACAGGATG CATGGTGCCC GCTTCCATCC TCCAGTGCCC CCAGTGTGCC AGAGATCACA 
GACTCTCCCA TTATGCACCC AGTGTTCAAA GCGGTCAAAG CCGGCATCCC CATTGGCTGT 
TTTCTGGCTA TTGAGATGAC CACCATCGGG CACAGTTTGG AGAAGTTTTG CAGTGAAGGA 
ATATCTTTGC TGAAGACTCT GATTCAGTCC CGTCATCTGA GAGCTGCTGT GCACATACTG 
GAGAACATTC TTCCTCTGGT CTACAACTGC CAGTTCTACC TGCTTAAGAA TGAGCAGTTT 
CTGAGCTGTG TCCAGCTGTT TTTGCAGCTG GATAGTGGCA CTCAAGGCGT AACCCAGCAG 
GTCACACAGA AGGTGGCCCA GCATCTGATT GGCCCCGCTA CATGTGAGAA TATCAAACTG 
CTGAACAGTG TGATCCAGGC TCATGTTCTG GAGAGCTCCA GGAATGGACG TGTTGGTGCA 
GTCGCTGTTC TGGAGTTCTG GCTTCAGGTT TTGACTGAAC AGAGCCTCTG GTATCGAGAC 
AGAGCTGTTC TCTTTCTTCT GGACCACATG TGCCGTTCTG CATTTCTTCA CCAACAAGAG 
GAATGTCTCC AGAAAATACT CTACCAGCAG CACAAGAGCG CTTTAGGTTA CCATGGAGAC 
AAAGGTTTGC TCTCGTCTAT TATGGGCTGG ATAGTGGCAG GAAATGTTAC TCCGTCTTTT 
ATTGAGGGCA GTTCTCTCAC AGGAGAGGTG TGGTTTGCTT GGATGATCCT AAACATGGAG 
ACAATGTTCG AGGAAGATTC CCAGCTGCGA CGCTGTGTAG AGCATGAGCT GCTCTCAAAC 
CCTGCGTGTA CACCAGATCA GGCTTTGAAG AAAGCCCAGG TGCGTCTCAA GCTTCAGGTT 
GTCCCATCAC TGCAGAGGCT GATGATCTAT CGCTGGGCTC AACAAGCTTT AGCCACTCCT 
GCAGACCATC CAATCCTGCC TTTGGTGTGG CAGAAATTCT TCCAGCTTTA CCTCCGTCAA 
CCTGGACCAG AGTTTGGGCT GGAGGTGAAA GGTTGCATTG GCAGGCGTTA TTTCCATAGT 
GCGGCCCATC TGTCCTTGCT GAAGAGCCTC AGACAGCGGC TGGTGGAAGT GTCTGATTTC 
CACCACGCCG CCAGCAAGGC TCTGAAAGTG CCCTGCGCTA GTGGAGATTC CGGTGATGGT 
CTGCTGACCC CCGGGACGCC CATCACACAG TACATGACCT CTCCTGACCT GCACACTGAG 
ATCGTGCGGC TCTTCACTGT GTTTGCCTTG TGGTTGGATG ATGAAAACCT TCAGAAGCAG 
GAAATTTACT TGCCCTCTTT GCCAAAGCAG TATGACACCC ATCGCCTGGC CAAAATTATG 
CAGCGCCAGC AGGAGGTGTG GATGGAGCAT GTGGATATCG AGCGAGTCCA GCACGAGCTG 
CAGGAAGTGC TCGATCTGTG GTCAAAGGTC AAGAGTGAAC CTGCAGTCTC ACAGAACAGC 
AGCTCCTCCA TCTTTACCGA CTTCATCAAC CCTCTGGCTG CAAGGGAGCG TATTCTCACT 
CACCTGAGAA AGCATGATGC CCCTCAGGGG TCGCTGCTGC CAGTGCCCAT GAAAGCTCCA 
GTGCCTGATA TTCCCACTGC GAGCCTAACT GATGAGAAAA TCTCCACCAC CTTAATTCAA 
GAGGACCTCA CTAAGCTCCA GCATCAAGCC AAGTTGGCAA CGGTTCGGGA AACCCAGCAG 
GTTGCTCTAG ACAATGAACT GTTGGAGGTG TTGCCACAGC TCTACATAAA CCGGGAAGAC 
CAGCTGTCAA TGCAGTTGGA GTGCCGTGGA AAAAGTGGAC AGCCCTGCCA AGGTCCTGCA 
AATATCACTG TTAAGTACTC AAGTATGCAC AAACTGGAGC CGGTGCAGAA TCAGATCCAG 
TCTCTGAAGA GTGACATTAA ACAGCTGCAG GCCGATGGAA TCAAAGCTCC TCCTCAGAGT 
CTGGCTGAGG CGGCCGTTCA CACTGAGAAC TTCATCACGG CCTTGGTTAA TGTTTTCAAG 
CAAAATCCAT CCCCTGGTGT ACTGAAGGTG GGCATCACGG CTTTCTATCA GATAGTGTCG 
TTTGTGTGTG AGGACACACA GAGACATCCA CCAACCCGCC AGTTCTTCAC ATCCTGCATT 
GAGATCCTCG GCCAGGTCTT TATTCGGGAC GTCCAATCAG AATGTAAACA CATGCTAAAG 
ACTATCCTAC TCAACAGACG TCTGTGTAAC CTACTGTCAC CATACTTCAC CCCGAACGCC 
TCTCCCTCAG AGCTGGTCTC TCTGTATGAA GAGGTGGTCA ACACCCTCCA CATCGACAGC 
GGTGATGTCA TATTCATGCT CCTCACAAAG TTTGACTTGT TTCAGTGGTT GTCGACGACC 
CAGCCTCCGT TCTCTGAGCG TAGTCGTCTG ATGACCGTCA TCCATCAGGC TCTCTGCACA 
TGTGGGCTGG AGCCTGAGCC CGAAGTCCTC ATGCCATTCA ACATCTTCTG CAAGCACTGG 
ACTGAACTGC TCCTCTACCA GTTTCCTGAC CACTACAGCG ACTTCCTCCG TCTCCTCATG 
CAGAGCTCTT CCGATCAGCT TCTTAGTCCT GACTGTTGGA GAACGTCACT GAAAGCTCTG 
GGCTGTTCTC CTCAAACTAA GAATAAGACA AACAAATCCC GCTCCATGCT CCTCTCCCCT 
CAACAGGTGG ATGAAACAAT CGAGTGGCTC TCAAAGTACT TCCTAAAGCT CCGACTGTCT 
AACGGGGACT TCAGGAGCTT TGGTCTTCTT TCAAAATGGG GGCCATACAT TGAGGAAGTC 
AAGGTGTTTT GGGAAGATCT GATTACCTAC GTCATTGATC TGGAACTAAA CCATTGCGGC 
AAAGAACCTG TAGGAAGTCC AAGACTGCTC AAAGCTCTGG AGAACCTGCA TAGAAAAATG 
GCCAAACTCT TTGAGCCGTG GATTTTGGTG CTTGACACTG ACAACACTAG TCATCCTCGC 
TGTTCTCCAT GGCTTGAATC AGACGCCGCA GTTGCAGCGA GCTTAGTAGG CTTATATACA 
CGCTTGACAG AGACTTTACA TGAGAAATTC AAAGATCGCT TGTTGCCAGG ACAGAGAGGA 
GCTCTGTGGC TTCATCTCAT GTTTTACTGT GAGACCTGCA CTTCTCCTAA AATGCCAGAG 
TATCTGCTGT ACACCTACCA CACTGAGTAC AGCCGTTTGC CCTGGAAGGA CCTGCATCCA 
GATCAGACAC TAATGAACCA GTTTTTCAAT GTGGAGAGGG GAAGTCCAAA GAGTTGTTTT 
GTGTTCATGG GAGAGTTGCT GTGTGAGGTG AACTGGGTTA GTGTATTGAG TGACTCTCTC 
AGCCCCCAGC TGAACCCCTC AACCCACACA ATGGTGGTCT CCGTACTCCA CCTCATGGTG 
TTTCTGGCCA AAGAGGATCT CAGTAAACCC GAGTCCTCTG TTCTCAGTCT GCTGGACCAG 
TCAGCTTCTC TGCCTTGGAA TCTGGTGGAT TTTTCTTCAT ATCAAAATGT CACGCGCTAT 
GTCAGCACAC ATTACCCAGC GTCCCTTGTT CTCAGTCACG ACACTTCTTC ACAGCAGGTC 
GTCAGATTGC TCAGAATGGC TGCTGGATTT GAGCCGACCA CACAAGTGTT GCATCACAAG 
GACATGACTC TAAAGTGTCA AGCCTTCCTT CACCTCATGG TGCAGTTCCT GACCTCGCTG 
GATCAAAATG GGAACATCAG TCTGGCATCG CTAGAGACAG AAATGGAGAA ATTACTGGAG 
AACATAGTCA TTTTCAACCC ACCTGAGACG GACCTCCAGC AGCGACACAT GGCCACCTGC 
AGTCTGTTTG CAGAGATGCT GACTCTGCTG AATGAGGCCG GTATCTCGAC AGCAGAGGGT 
CTGGGCACTA AACTGCACTG CTGGGTGGAG AAGCGGGCTC GAGGGCCTCT GCTTCTGCCT 
TTGCTAACGG CTGCGTGCAG ATGCCTGGCT TCAGTGCGTC ACATGACACG CACCACCGAG 
GCCTGTATTC TCTCCTACTT CACCGATGGA TGTGTGGATC AGTACTCTGG ATGGGGGCCC 
ATCCTGGTGT CTCTTCAGGT TCCTGAACTG ACTGTTGAGG ACTTCATTCA GGAAAGCCTA 
AGTCTGGGCA GCTTCCTCAC GCTGTACGTC TACATCCTCC AGAGGCTAAA CCTGGAGCAA 
ACCCTCCTCA ATGAGAAGAA AACCTTAATG CTGCTCAATA CGTGGATCAG TCAGGTGTTT 
CCCAGTGGTC CAGCTGATGA GGCCAAGCTG TTTTTATGGT GGCATAAATT CTTGGAGCTT 
CTGCAAATCC AAGTGGACCA AGAGGATGCC TGCTCACTGG ACACTCTCAT TCTCACCTTA 
ATGGCGTTTC AGAATCGTCT CGTTCAACTC GGAGAGGAAA GACTAAATTC CGGCATCCTG 
GGAGCCATTG GCCTTGGGAG GAAATCACCA CTTTCTAACA GGTTTCGGGT TGTGGCGCGC 
AGCATGTCAG CCTTTCTGCT GGTTCAGGTG CCCTCAGAGA ATCAGCTGCG TCTTCATCCC 
GGGACAGAGC TGCAGCTGTC AGCTAAAGCC CAGCAGGCCT TGTCTGCACT GGAGGCGATG 
GCCAGTAATA AGCAGTATTC AGAGCTGCAT GTGTCTGTGA ACCAGGCCTG TCAGTTCATC 
AAGTACCCGA ATCACTGTCT GCGAGACGCC AACACTCTTC TCACTCTGCT GGTCAATGCA 
CTCTACACTG ACCTTCATTA CCTGGACATC ATCCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001103995.1
CDS	1..7599
Translation	MLHLQVKKERQSESVRQPSGSSKAVNEPEFTDVPLSLPHVPPDLNTAASQTSLDHHDKAENEQQHVLLCEKPEVVQKSTCHEDKEHELPSQDAAPRLDQTSVLAPSSQSDPKPLSAKLEPFSVPSIYPSIPASLSQPQLSEDSYSISYELLDGSLMPAASERLVAPAVLTLANQESSPPSLVPVEVVDVERSRERLYPELPKTCQSVKPFTSEQLRMWEPGSWLENVELHEAEFQNLAHQEGHELYELLLSYWRCRKQLTQAQTELQAANSDCKNVQNRLWSFKDERLSLQGICADQNKVYGYHCYQQVTLNEAALAELKHLFSAKAEILHQTVALHSYTSILSRLQVESYLYRLLSSNPTTKSVAVQDTSAGNPKSQPSSLQPLKESISVLFIFTRRVLDDSQFQADIHLWLQRLVSVLHQVGSTGDHLFILNHLLCCPAGVGKWAVPFLQIKVLDNPSGVYHFMQALAILMSPARHRPDFLGHMKPSDARGNNSLTEPASGNWTLVDEGGEEDEDPETSWMLLSEDDLVSLFSQFPFDELFKHLLGICSKGDYQPLATTSQKMMKIFAFASSLVELLAVGLQTYNRARYRQFVKRIGHMIRMTLCYVSDHWAQYISLTGVSRTTVQEQSFSMEKLQVEFDHLFLRAVLHVLKAKRLGIWLFMSEMPYGTLSSSMLWKIFYIMQCAETDGLEKLSCSLSAEDCIQRLRESSHQERFQLWLSEINSSDGICLLTTFAHMAQPRRTDVDPEFVRNIVLEIYEVSYVSVTTREIFSKVGRELLAAIAAAHPHIISVLLERLRETIERVGMVSLYLFKELPLHLWMPAQEEVGLIRDWLMNFSLTAVENRLACIILEGLNWGFQPNGCLILPAPLHSDVALLVIEAYQKYLTDKPYGGILSEGIKQVSYLANVVRLGQTPESSFNQWAWDIVLRLKLHANERSPQDAWCPLPSSSAPSVPEITDSPIMHPVFKAVKAGIPIGCFLAIEMTTIGHSLEKFCSEGISLLKTLIQSRHLRAAVHILENILPLVYNCQFYLLKNEQFLSCVQLFLQLDSGTQGVTQQVTQKVAQHLIGPATCENIKLLNSVIQAHVLESSRNGRVGAVAVLEFWLQVLTEQSLWYRDRAVLFLLDHMCRSAFLHQQEECLQKILYQQHKSALGYHGDKGLLSSIMGWIVAGNVTPSFIEGSSLTGEVWFAWMILNMETMFEEDSQLRRCVEHELLSNPACTPDQALKKAQVRLKLQVVPSLQRLMIYRWAQQALATPADHPILPLVWQKFFQLYLRQPGPEFGLEVKGCIGRRYFHSAAHLSLLKSLRQRLVEVSDFHHAASKALKVPCASGDSGDGLLTPGTPITQYMTSPDLHTEIVRLFTVFALWLDDENLQKQEIYLPSLPKQYDTHRLAKIMQRQQEVWMEHVDIERVQHELQEVLDLWSKVKSEPAVSQNSSSSIFTDFINPLAARERILTHLRKHDAPQGSLLPVPMKAPVPDIPTASLTDEKISTTLIQEDLTKLQHQAKLATVRETQQVALDNELLEVLPQLYINREDQLSMQLECRGKSGQPCQGPANITVKYSSMHKLEPVQNQIQSLKSDIKQLQADGIKAPPQSLAEAAVHTENFITALVNVFKQNPSPGVLKVGITAFYQIVSFVCEDTQRHPPTRQFFTSCIEILGQVFIRDVQSECKHMLKTILLNRRLCNLLSPYFTPNASPSELVSLYEEVVNTLHIDSGDVIFMLLTKFDLFQWLSTTQPPFSERSRLMTVIHQALCTCGLEPEPEVLMPFNIFCKHWTELLLYQFPDHYSDFLRLLMQSSSDQLLSPDCWRTSLKALGCSPQTKNKTNKSRSMLLSPQQVDETIEWLSKYFLKLRLSNGDFRSFGLLSKWGPYIEEVKVFWEDLITYVIDLELNHCGKEPVGSPRLLKALENLHRKMAKLFEPWILVLDTDNTSHPRCSPWLESDAAVAASLVGLYTRLTETLHEKFKDRLLPGQRGALWLHLMFYCETCTSPKMPEYLLYTYHTEYSRLPWKDLHPDQTLMNQFFNVERGSPKSCFVFMGELLCEVNWVSVLSDSLSPQLNPSTHTMVVSVLHLMVFLAKEDLSKPESSVLSLLDQSASLPWNLVDFSSYQNVTRYVSTHYPASLVLSHDTSSQQVVRLLRMAAGFEPTTQVLHHKDMTLKCQAFLHLMVQFLTSLDQNGNISLASLETEMEKLLENIVIFNPPETDLQQRHMATCSLFAEMLTLLNEAGISTAEGLGTKLHCWVEKRARGPLLLPLLTAACRCLASVRHMTRTTEACILSYFTDGCVDQYSGWGPILVSLQVPELTVEDFIQESLSLGSFLTLYVYILQRLNLEQTLLNEKKTLMLLNTWISQVFPSGPADEAKLFLWWHKFLELLQIQVDQEDACSLDTLILTLMAFQNRLVQLGEERLNSGILGAIGLGRKSPLSNRFRVVARSMSAFLLVQVPSENQLRLHPGTELQLSAKAQQALSALEAMASNKQYSELHVSVNQACQFIKYPNHCLRDANTLLTLLVNALYTDLHYLDIIR

Target ORF information:

RefSeq Version	NM_001110525.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ectopic P-granules autophagy protein 5 homolog (C. elegans) (epg5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001110525.1

ORF Insert Sequence:

ATGCTTCATT TACAGGTCAA AAAGGAGAGG CAGTCAGAGA GCGTCAGACA GCCCTCGGGA 
TCTTCTAAAG CAGTTAATGA GCCTGAGTTC ACTGATGTGC CGCTCAGCCT CCCTCATGTG 
CCTCCTGACC TCAACACAGC TGCCTCTCAG ACTTCACTAG ATCATCATGA CAAAGCTGAA 
AATGAGCAAC AGCATGTTCT CTTATGCGAG AAGCCTGAAG TAGTACAGAA ATCGACGTGT 
CATGAAGACA AAGAGCATGA ATTGCCATCA CAAGATGCTG CACCTCGGCT GGACCAAACA 
TCAGTGCTTG CTCCGTCCAG CCAAAGTGAT CCCAAACCCC TCAGTGCTAA ACTTGAGCCA 
TTTAGTGTCC CCTCTATATA TCCCTCTATC CCAGCTTCCC TCTCCCAGCC TCAGCTTTCA 
GAGGACTCGT ACTCTATCTC CTACGAACTG TTAGATGGCT CTTTAATGCC AGCAGCGTCT 
GAGAGACTAG TAGCTCCGGC TGTTTTGACT CTGGCTAACC AGGAGTCCTC TCCGCCCAGC 
TTGGTTCCAG TGGAGGTTGT GGATGTGGAA AGATCTAGAG AGAGGCTTTA CCCAGAGCTG 
CCTAAGACCT GCCAGTCTGT GAAGCCCTTC ACTAGTGAGC AGCTGCGCAT GTGGGAGCCC 
GGTTCCTGGC TGGAAAACGT GGAGCTGCAT GAAGCCGAAT TCCAGAATCT GGCTCATCAA 
GAGGGACATG AGCTGTACGA GCTGCTGCTG AGCTACTGGA GGTGCCGGAA GCAGCTGACG 
CAGGCCCAGA CTGAACTGCA GGCTGCCAAC TCGGACTGCA AAAATGTGCA GAATCGCCTG 
TGGAGCTTCA AGGATGAAAG GCTTTCGTTA CAGGGTATAT GTGCAGACCA GAATAAGGTG 
TATGGCTACC ACTGCTACCA GCAGGTGACA CTGAACGAGG CTGCGTTAGC TGAGCTGAAG 
CACCTGTTTT CTGCTAAAGC GGAAATCCTC CATCAGACAG TGGCTCTGCA CTCATACACC 
TCCATCCTGT CCCGTCTGCA AGTGGAGTCT TATCTCTACC GCTTGCTCAG CAGTAACCCC 
ACCACCAAGA GTGTGGCTGT GCAAGATACA AGTGCAGGCA ATCCAAAGTC TCAGCCCTCG 
TCTTTGCAGC CGCTGAAGGA AAGCATCAGT GTGCTGTTCA TCTTCACCCG ACGGGTCCTA 
GATGATTCTC AGTTTCAGGC TGACATCCAT CTTTGGCTTC AGAGGCTGGT GTCTGTGCTG 
CATCAGGTCG GTTCTACAGG AGATCATCTA TTCATCCTAA ACCACCTGCT CTGCTGCCCG 
GCAGGTGTAG GGAAATGGGC TGTGCCATTC CTACAGATCA AAGTATTGGA TAACCCATCT 
GGAGTATATC ATTTCATGCA GGCCCTTGCT ATCCTTATGT CTCCAGCAAG GCACCGTCCA 
GACTTCCTGG GACACATGAA GCCCAGTGAT GCAAGGGGCA ACAACAGCCT AACAGAACCT 
GCATCTGGTA ACTGGACTCT AGTGGATGAA GGTGGAGAAG AGGACGAAGA CCCAGAAACG 
AGCTGGATGC TGCTGTCTGA GGATGATCTG GTTTCTCTGT TCTCTCAGTT TCCCTTTGAT 
GAGCTCTTCA AACATTTACT GGGCATCTGC TCTAAAGGGG ACTATCAGCC TCTAGCCACC 
ACAAGTCAAA AGATGATGAA GATATTTGCG TTTGCCTCTT CACTGGTTGA GTTGCTAGCA 
GTCGGCTTGC AGACCTACAA CAGAGCACGC TATCGGCAGT TTGTGAAGAG GATTGGTCAC 
ATGATTCGGA TGACGCTCTG CTATGTGAGT GACCACTGGG CGCAGTACAT CAGTCTGACC 
GGTGTGAGCA GGACCACAGT GCAGGAGCAG TCATTTTCAA TGGAGAAACT GCAGGTGGAG 
TTTGACCATC TCTTCCTGAG GGCGGTGCTG CATGTGCTTA AGGCCAAGAG ACTGGGCATA 
TGGCTGTTCA TGTCTGAGAT GCCGTATGGG ACATTGTCCA GCTCCATGCT GTGGAAGATC 
TTCTACATTA TGCAGTGTGC AGAGACAGAT GGTCTGGAGA AGCTCAGTTG TAGCTTGAGT 
GCTGAGGACT GTATCCAGAG GCTCAGAGAA TCAAGCCATC AGGAGAGGTT TCAGCTTTGG 
CTGTCTGAGA TAAACAGCTC AGATGGGATC TGCCTGTTGA CAACGTTTGC GCACATGGCC 
CAGCCGAGAC GCACCGATGT AGACCCTGAG TTTGTCAGGA ACATAGTGCT AGAGATATAC 
GAGGTTTCCT ATGTAAGTGT CACCACCCGT GAGATCTTCT CTAAAGTTGG ACGGGAATTG 
CTTGCAGCGA TAGCGGCTGC CCACCCTCAC ATCATCTCTG TGTTGCTGGA GAGACTGAGA 
GAGACCATAG AGAGAGTGGG CATGGTGTCG TTGTACCTGT TTAAAGAGTT GCCCTTGCAC 
TTGTGGATGC CAGCTCAGGA GGAGGTGGGG CTGATCAGAG ACTGGCTGAT GAACTTCAGT 
CTCACTGCTG TTGAGAACAG ATTAGCCTGC ATTATACTAG AGGGCCTCAA CTGGGGATTT 
CAGCCAAATG GTTGCCTTAT TTTACCTGCA CCTCTGCACA GTGACGTTGC CTTGTTGGTG 
ATTGAGGCAT ATCAAAAATA TCTTACAGAT AAACCATATG GTGGCATCCT CTCAGAAGGC 
ATTAAACAGG TGTCTTACCT GGCTAACGTG GTGAGGCTCG GCCAGACTCC AGAATCTTCC 
TTTAATCAGT GGGCTTGGGA TATAGTTCTG AGGCTAAAGC TCCACGCCAA TGAAAGAAGC 
CCACAGGATG CATGGTGCCC GCTTCCATCC TCCAGTGCCC CCAGTGTGCC AGAGATCACA 
GACTCTCCCA TTATGCACCC AGTGTTCAAA GCGGTCAAAG CCGGCATCCC CATTGGCTGT 
TTTCTGGCTA TTGAGATGAC CACCATCGGG CACAGTTTGG AGAAGTTTTG CAGTGAAGGA 
ATATCTTTGC TGAAGACTCT GATTCAGTCC CGTCATCTGA GAGCTGCTGT GCACATACTG 
GAGAACATTC TTCCTCTGGT CTACAACTGC CAGTTCTACC TGCTTAAGAA TGAGCAGTTT 
CTGAGCTGTG TCCAGCTGTT TTTGCAGCTG GATAGTGGCA CTCAAGGCGT AACCCAGCAG 
GTCACACAGA AGGTGGCCCA GCATCTGATT GGCCCCGCTA CATGTGAGAA TATCAAACTG 
CTGAACAGTG TGATCCAGGC TCATGTTCTG GAGAGCTCCA GGAATGGACG TGTTGGTGCA 
GTCGCTGTTC TGGAGTTCTG GCTTCAGGTT TTGACTGAAC AGAGCCTCTG GTATCGAGAC 
AGAGCTGTTC TCTTTCTTCT GGACCACATG TGCCGTTCTG CATTTCTTCA CCAACAAGAG 
GAATGTCTCC AGAAAATACT CTACCAGCAG CACAAGAGCG CTTTAGGTTA CCATGGAGAC 
AAAGGTTTGC TCTCGTCTAT TATGGGCTGG ATAGTGGCAG GAAATGTTAC TCCGTCTTTT 
ATTGAGGGCA GTTCTCTCAC AGGAGAGGTG TGGTTTGCTT GGATGATCCT AAACATGGAG 
ACAATGTTCG AGGAAGATTC CCAGCTGCGA CGCTGTGTAG AGCATGAGCT GCTCTCAAAC 
CCTGCGTGTA CACCAGATCA GGCTTTGAAG AAAGCCCAGG TGCGTCTCAA GCTTCAGGTT 
GTCCCATCAC TGCAGAGGCT GATGATCTAT CGCTGGGCTC AACAAGCTTT AGCCACTCCT 
GCAGACCATC CAATCCTGCC TTTGGTGTGG CAGAAATTCT TCCAGCTTTA CCTCCGTCAA 
CCTGGACCAG AGTTTGGGCT GGAGGTGAAA GGTTGCATTG GCAGGCGTTA TTTCCATAGT 
GCGGCCCATC TGTCCTTGCT GAAGAGCCTC AGACAGCGGC TGGTGGAAGT GTCTGATTTC 
CACCACGCCG CCAGCAAGGC TCTGAAAGTG CCCTGCGCTA GTGGAGATTC CGGTGATGGT 
CTGCTGACCC CCGGGACGCC CATCACACAG TACATGACCT CTCCTGACCT GCACACTGAG 
ATCGTGCGGC TCTTCACTGT GTTTGCCTTG TGGTTGGATG ATGAAAACCT TCAGAAGCAG 
GAAATTTACT TGCCCTCTTT GCCAAAGCAG TATGACACCC ATCGCCTGGC CAAAATTATG 
CAGCGCCAGC AGGAGGTGTG GATGGAGCAT GTGGATATCG AGCGAGTCCA GCACGAGCTG 
CAGGAAGTGC TCGATCTGTG GTCAAAGGTC AAGAGTGAAC CTGCAGTCTC ACAGAACAGC 
AGCTCCTCCA TCTTTACCGA CTTCATCAAC CCTCTGGCTG CAAGGGAGCG TATTCTCACT 
CACCTGAGAA AGCATGATGC CCCTCAGGGG TCGCTGCTGC CAGTGCCCAT GAAAGCTCCA 
GTGCCTGATA TTCCCACTGC GAGCCTAACT GATGAGAAAA TCTCCACCAC CTTAATTCAA 
GAGGACCTCA CTAAGCTCCA GCATCAAGCC AAGTTGGCAA CGGTTCGGGA AACCCAGCAG 
GTTGCTCTAG ACAATGAACT GTTGGAGGTG TTGCCACAGC TCTACATAAA CCGGGAAGAC 
CAGCTGTCAA TGCAGTTGGA GTGCCGTGGA AAAAGTGGAC AGCCCTGCCA AGGTCCTGCA 
AATATCACTG TTAAGTACTC AAGTATGCAC AAACTGGAGC CGGTGCAGAA TCAGATCCAG 
TCTCTGAAGA GTGACATTAA ACAGCTGCAG GCCGATGGAA TCAAAGCTCC TCCTCAGAGT 
CTGGCTGAGG CGGCCGTTCA CACTGAGAAC TTCATCACGG CCTTGGTTAA TGTTTTCAAG 
CAAAATCCAT CCCCTGGTGT ACTGAAGGTG GGCATCACGG CTTTCTATCA GATAGTGTCG 
TTTGTGTGTG AGGACACACA GAGACATCCA CCAACCCGCC AGTTCTTCAC ATCCTGCATT 
GAGATCCTCG GCCAGGTCTT TATTCGGGAC GTCCAATCAG AATGTAAACA CATGCTAAAG 
ACTATCCTAC TCAACAGACG TCTGTGTAAC CTACTGTCAC CATACTTCAC CCCGAACGCC 
TCTCCCTCAG AGCTGGTCTC TCTGTATGAA GAGGTGGTCA ACACCCTCCA CATCGACAGC 
GGTGATGTCA TATTCATGCT CCTCACAAAG TTTGACTTGT TTCAGTGGTT GTCGACGACC 
CAGCCTCCGT TCTCTGAGCG TAGTCGTCTG ATGACCGTCA TCCATCAGGC TCTCTGCACA 
TGTGGGCTGG AGCCTGAGCC CGAAGTCCTC ATGCCATTCA ACATCTTCTG CAAGCACTGG 
ACTGAACTGC TCCTCTACCA GTTTCCTGAC CACTACAGCG ACTTCCTCCG TCTCCTCATG 
CAGAGCTCTT CCGATCAGCT TCTTAGTCCT GACTGTTGGA GAACGTCACT GAAAGCTCTG 
GGCTGTTCTC CTCAAACTAA GAATAAGACA AACAAATCCC GCTCCATGCT CCTCTCCCCT 
CAACAGGTGG ATGAAACAAT CGAGTGGCTC TCAAAGTACT TCCTAAAGCT CCGACTGTCT 
AACGGGGACT TCAGGAGCTT TGGTCTTCTT TCAAAATGGG GGCCATACAT TGAGGAAGTC 
AAGGTGTTTT GGGAAGATCT GATTACCTAC GTCATTGATC TGGAACTAAA CCATTGCGGC 
AAAGAACCTG TAGGAAGTCC AAGACTGCTC AAAGCTCTGG AGAACCTGCA TAGAAAAATG 
GCCAAACTCT TTGAGCCGTG GATTTTGGTG CTTGACACTG ACAACACTAG TCATCCTCGC 
TGTTCTCCAT GGCTTGAATC AGACGCCGCA GTTGCAGCGA GCTTAGTAGG CTTATATACA 
CGCTTGACAG AGACTTTACA TGAGAAATTC AAAGATCGCT TGTTGCCAGG ACAGAGAGGA 
GCTCTGTGGC TTCATCTCAT GTTTTACTGT GAGACCTGCA CTTCTCCTAA AATGCCAGAG 
TATCTGCTGT ACACCTACCA CACTGAGTAC AGCCGTTTGC CCTGGAAGGA CCTGCATCCA 
GATCAGACAC TAATGAACCA GTTTTTCAAT GTGGAGAGGG GAAGTCCAAA GAGTTGTTTT 
GTGTTCATGG GAGAGTTGCT GTGTGAGGTG AACTGGGTTA GTGTATTGAG TGACTCTCTC 
AGCCCCCAGC TGAACCCCTC AACCCACACA ATGGTGGTCT CCGTACTCCA CCTCATGGTG 
TTTCTGGCCA AAGAGGATCT CAGTAAACCC GAGTCCTCTG TTCTCAGTCT GCTGGACCAG 
TCAGCTTCTC TGCCTTGGAA TCTGGTGGAT TTTTCTTCAT ATCAAAATGT CACGCGCTAT 
GTCAGCACAC ATTACCCAGC GTCCCTTGTT CTCAGTCACG ACACTTCTTC ACAGCAGGTC 
GTCAGATTGC TCAGAATGGC TGCTGGATTT GAGCCGACCA CACAAGTGTT GCATCACAAG 
GACATGACTC TAAAGTGTCA AGCCTTCCTT CACCTCATGG TGCAGTTCCT GACCTCGCTG 
GATCAAAATG GGAACATCAG TCTGGCATCG CTAGAGACAG AAATGGAGAA ATTACTGGAG 
AACATAGTCA TTTTCAACCC ACCTGAGACG GACCTCCAGC AGCGACACAT GGCCACCTGC 
AGTCTGTTTG CAGAGATGCT GACTCTGCTG AATGAGGCCG GTATCTCGAC AGCAGAGGGT 
CTGGGCACTA AACTGCACTG CTGGGTGGAG AAGCGGGCTC GAGGGCCTCT GCTTCTGCCT 
TTGCTAACGG CTGCGTGCAG ATGCCTGGCT TCAGTGCGTC ACATGACACG CACCACCGAG 
GCCTGTATTC TCTCCTACTT CACCGATGGA TGTGTGGATC AGTACTCTGG ATGGGGGCCC 
ATCCTGGTGT CTCTTCAGGT TCCTGAACTG ACTGTTGAGG ACTTCATTCA GGAAAGCCTA 
AGTCTGGGCA GCTTCCTCAC GCTGTACGTC TACATCCTCC AGAGGCTAAA CCTGGAGCAA 
ACCCTCCTCA ATGAGAAGAA AACCTTAATG CTGCTCAATA CGTGGATCAG TCAGGTGTTT 
CCCAGTGGTC CAGCTGATGA GGCCAAGCTG TTTTTATGGT GGCATAAATT CTTGGAGCTT 
CTGCAAATCC AAGTGGACCA AGAGGATGCC TGCTCACTGG ACACTCTCAT TCTCACCTTA 
ATGGCGTTTC AGAATCGTCT CGTTCAACTC GGAGAGGAAA GACTAAATTC CGGCATCCTG 
GGAGCCATTG GCCTTGGGAG GAAATCACCA CTTTCTAACA GGTTTCGGGT TGTGGCGCGC 
AGCATGTCAG CCTTTCTGCT GGTTCAGGTG CCCTCAGAGA ATCAGCTGCG TCTTCATCCC 
GGGACAGAGC TGCAGCTGTC AGCTAAAGCC CAGCAGGCCT TGTCTGCACT GGAGGCGATG 
GCCAGTAATA AGCAGTATTC AGAGCTGCAT GTGTCTGTGA ACCAGGCCTG TCAGTTCATC 
AAGTACCCGA ATCACTGTCT GCGAGACGCC AACACTCTTC TCACTCTGCT GGTCAATGCA 
CTCTACACTG ACCTTCATTA CCTGGACATC ATCCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EPG5 ( XM_005165361.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005165418.1 Danio rerio ectopic P-granules autophagy protein 5 homolog (C. elegans) (epg5), transcript variant X1, mRNA.

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CloneID	ODa10746
Clone ID Related Accession (Same CDS sequence)	XM_005165361.4 , XM_005165361.3
Accession Version	XM_005165361.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ectopic P granules protein 5 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165361.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG TGAGACCTAA AAAGGCAAAG TCCAAATCCA GTGGGAAGCC ACAGGTCAAA 
AAGGAGAGGC AGTCAGAGAG CGTCAGACAG CCCTCGGGAT CTTCTAAAGC AGTTAATGAG 
CCTGAGTTCA CTGATGTGCC GCTCAGCCTC CCTCATGTGC CTCCTGACCT CAACACAGCT 
GCCTCTCAGA CTTCACTAGA TCATCATGAC AAAGCTGAAA ATGAGCAACA GCATGTTCTC 
TTATGCGAGA AGCCTGAAGT AGTACAGAAA TCGACGTGTC ATGAAGACAA AGAGCATGAA 
TTGCCATCAC AAGATGCTGC ACCTCGGCTG GACCAAACAT CAGTGCTTGC TCCGTCCAGC 
CAAAGTGATC CCAAACCCCT CAGTGCTAAA CTTGAGCCAT TTAGTGTCCC CTCTATATAT 
CCCTCTATCC CAGCTTCCCT CTCCCAGCCT CAGCTTTCAG AGGACTCGTA CTCTATCTCC 
TACGAACTGT TAGATGGCTC TTTAATGCCA GCAGCGTCTG AGAGACTAGT AGCTCCGGCT 
GTTTTGACTC TGGCTAACCA GGAGTCCTCT CCGCCCAGCT TGGTTCCAGT GGAGGTTGTG 
GATGTGGAAA GATCTAGAGA GAGGCTTTAC CCAGAGCTGC CTAAGACCTG CCAGTCTGTG 
AAGCCCTTCA CTAGTGAGCA GCTGCGCATG TGGGAGCCCG GTTCCTGGCT GGAAAACGTG 
GAGCTGCATG AAGCCGAATT CCAGAATCTG GCTCATCAAG AGGGACATGA GCTGTACGAG 
CTGCTGCTGA GCTACTGGAG GTGCCGGAAG CAGCTGACGC AGGCCCAGAC TGAACTGCAG 
GCTGCCAACT CGGACTGCAA AAATGTGCAG AATCGCCTGT GGAGCTTCAA GGATGAAAGG 
CTTTCGTTAC AGGGTATATG TGCAGACCAG AATAAGGTGT ATGGCTACCA CTGCTACCAG 
CAGGTGACAC TGAACGAGGC TGCGTTAGCT GAGCTGAAGC ACCTGTTTTC TGCTAAAGCG 
GAAATCCTCC ATCAGACAGT GGCTCTGCAC TCATACACCT CCATCCTGTC CCGTCTGCAA 
GTGGAGTCTT ATCTCTACCG CTTGCTCAGC AGTAACCCCA CCACCAAGAG TGTGGCTGTG 
CAAGATACAA GTGCAGTGAG TCCAAAGTCT CAGCCCTCGT CTTTGCAGCC GCTGAAGGAA 
AGCATCAGTG TGCTGTTCAT CTTCACCCGA CGGGTCCTAG ATGATTCTCA GTTTCAGGCT 
GACATCCATC TTTGGCTTCA GAGGCTGGTG TCTGTGCTGC ATCAGGTCGG TTCTACAGGA 
GATCATCTAT TCATCCTAAA CCACCTGCTC TGCTGCCCGG CAGGTGTAGG GAAATGGGCT 
GTGCCATTCC TACAGATCAA AGTATTGGAT AACCCATCTG GAGTATATCA TTTCATGCAG 
GCCCTTGCTA TCCTTATGTC TCCAGCAAGG CACCGTCCAG ACTTCCTGGG ACACATGAAG 
CCCAGTGATG CAAGGGGCAA CAACAGCCTA ACAGAACCTG CATCTGGTAA CTGGACTCTA 
GTGGATGAAG GTGGAGAAGA GGACGAAGAC CCAGAAACGA GCTGGATGCT GCTGTCTGAG 
GATGATCTGG TTTCTCTGTT CTCTCAGTTT CCCTTTGATG AGCTCTTCAA ACATTTACTG 
GGCATCTGCT CTAAAGGGGA CTATCAGCCT CTAGCCACCA CAAGTCAAAA GATGATGAAG 
ATATTTGCGT TTGCCTCTTC ACTGGTTGAG TTGCTAGCAG TCGGCTTGCA GACCTACAAC 
AGAGCACGCT ATCGGCAGTT TGTGAAGAGG ATTGGTCACA TGATTCGGAT GACGCTCTGC 
TATGTGAGTG ACCACTGGGC GCAGTACATC AGTCTGACCG GTGTGAGCAG GACCACAGTG 
CAGGAGCAGT CATTTTCAAT GGAGAAACTG CAGGTGGAGT TTGACCATCT CTTCCTGAGG 
GCGGTGCTGC ATGTGCTTAA GGCCAAGAGA CTGGGCATAT GGCTGTTCAT GTCTGAGATG 
CCGTATGGGA CATTGTCCAG CTCCATGCTG TGGAAGATCT TCTACATTAT GCAGTGTGCA 
GAGACAGATG GTCTGGAGAA GCTCAGTTGT AGCTTGAGTG CTGAGGACTG TATCCAGAGG 
CTCAGAGAAT CAAGCCATCA GGAGAGGTTT CAGCTTTGGC TGTCTGAGAT AAACAGCTCA 
GATGGGATCT GCCTGTTGAC AACGTTTGCG CACATGGCCC AGCCGAGACG CACCGATGTA 
GACCCTGAGT TTGTCAGGAA CATAGTGCTA GAGATATACG AGGTTTCCTA TGTAAGTGTC 
ACCACCCGTG AGATCTTCTC TAAAGTTGGA CGGGAATTGC TTGCAGCGAT AGCGGCTGCC 
CACCCTCACA TCATCTCTGT GTTGCTGGAG AGACTGAGAG AGACCATAGA GAGAGTGGGC 
ATGGTGTCGT TGTACCTGTT TAAAGAGTTG CCCTTGCACT TGTGGATGCC AGCTCAGGAG 
GAGGTGGGGC TGATCAGAGA CTGGCTGATG AACTTCAGTC TCACTGCTGT TGAGAACAGA 
TTAGCCTGCA TTATACTAGA GGGCCTCAAC TGGGGATTTC AGCCAAATGG TTGCCTTATT 
TTACCTGCAC CTCTGCACAG TGACGTTGCC TTGTTGGTGA TTGAGGCATA TCAAAAATAT 
CTTACAGATA AACCATATGG TGGCATCCTC TCAGAAGGCA TTAAACAGGT GTCTTACCTG 
GCTAACGTGG TGAGGCTCGG CCAGACTCCA GAATCTTCCT TTAATCAGTG GGCTTGGGAT 
ATAGTTCTGA GGCTAAAGCT CCACGCCAAT GAAAGAAGCC CACAGGATGC ATGGTGCCCG 
CTTCCATCCT CCAGTGCCCC CAGTGTGCCA GAGATCACAG ACTCTCCCAT TATGCACCCA 
GTGTTCAAAG CGGTCAAAGC CGGCATCCCC ATTGGCTGTT TTCTGGCTAT TGAGATGACC 
ACCATCGGGC ACAGTTTGGA GAAGTTTTGC AGTGAAGGAA TATCTTTGCT GAAGACTCTG 
ATTCAGTCCC GTCATCTGAG AGCTGCTGTG CACATACTGG AGAACATTCT TCCTCTGGTC 
TACAACTGCC AGTTCTACCT GCTTAAGAAT GAGCAGTTTC TGAGCTGTGT CCAGCTGTTT 
TTGCAGCTGG ATAGTGGCAC TCAAGGCGTA ACCCAGCAGG TCACACAGAA GGTGGCCCAG 
CATCTGATTG GCCCCGCTAC ATGTGAGAAT ATCAAACTGC TGAACAGTGT GATCCAGGCT 
CATGTTCTGG AGAGCTCCAG GAATGGACGT GTTGGTGCAG TCGCTGTTCT GGAGTTCTGG 
CTTCAGGTTT TGACTGAACA GAGCCTCTGG TATCGAGACA GAGCTGTTCT CTTTCTTCTG 
GACCACATGT GCCGTTCTGC ATTTCTTCAC CAACAAGAGG AATGTCTCCA GAAAATACTC 
TACCAGCAGC ACAAGAGCGC TTTAGGTTAC CATGGAGACA AAGGTTTGCT CTCGTCTATT 
ATGGGCTGGA TAGTGGCAGG AAATGTTACT CCGTCTTTTA TTGAGGGCAG TTCTCTCACA 
GGAGAGGTGT GGTTTGCTTG GATGATCCTA AACATGGAGA CAATGTTCGA GGAAGATTCC 
CAGCTGCGAC GCTGTGTAGA GCATGAGCTG CTCTCAAACC CTGCGTGTAC ACCAGATCAG 
GCTTTGAAGA AAGCCCAGGT GCGTCTCAAG CTTCAGGTTG TCCCATCACT GCAGAGGCTG 
ATGATCTATC GCTGGGCTCA ACAAGCTTTA GCCACTCCTG CAGACCATCC AATCCTGCCT 
TTGGTGTGGC AGAAATTCTT CCAGCTTTAC CTCCGTCAAC CTGGACCAGA GTTTGGGCTG 
GAGGTGAAAG GTTGCATTGG CAGGCGTTAT TTCCATAGTG CGGCCCATCT GTCCTTGCTG 
AAGAGCCTCA GACAGCGGCT GGTGGAAGTG TCTGATTTCC ACCACGCCGC CAGCAAGGCT 
CTGAAAGTGC CCTGCGCTAG TGGAGATTCC GGTGATGGTC TGCTGACCCC CGGGACGCCC 
ATCACACAGT ACATGACCTC TCCTGACCTG CACACTGAGA TCGTGCGGCT CTTCACTGTG 
TTTGCCTTGT GGTTGGATGA TGAAAACCTT CAGAAGCAGG AAATTTACTT GCCCTCTTTG 
CCAAAGCAGT ATGACACCCA TCGCCTGGCC AAAATTATGC AGCGCCAGCA GTATTTTGAG 
GTGTGGATGG AGCATGTGGA TATCGAGCGA GTCCAGCACG AGCTGCAGGA AGTGCTCGAT 
CTGTGGTCAA AGGTCAAGAG TGAACCTGCA GTCTCACAGA ACAGCAGCTC CTCCATCTTT 
ACCGACTTCA TCAACCCTCT GGCTGCAAGG GAGCGTATTC TCACTCACCT GAGAAAGCAT 
GATGCCCCTC AGGGGTCGCT GCTGCCAGTG CCCATGAAAG CTCCAGTGCC TGATATTCCC 
ACTGCGAGCC TAACTGATGA GAAAATCTCC ACCACCTTAA TTCAAGAGGA CCTCACTAAG 
CTCCAGCATC AAGCCAAGTT GGCAACGGTT CGGGAAACCC AGCAGGTTGC TCTAGACAAT 
GAACTGTTGG AGGTGTTGCC ACAGCTCTAC ATAAACCGGG AAGACCAGCT GTCAATGCAG 
TTGGAGTGCC GTGGAAAAAG TGGACAGCCC TGCCAAGGTC CTGCAAATAT CACTGTTAAG 
TACTCAAGTA TGCACAAACT GGAGCCGGTG CAGAATCAGA TCCAGTCTCT GAAGAGTGAC 
ATTAAACAGC TGCAGGCCGA TGGAATCAAA GCTCCTCCTC AGAGTCTGGC TGAGGCGGCC 
GTTCACACTG AGAACTTCAT CACGGCCTTG GTTAATGTTT TCAAGCAAAA TCCATCCCCT 
GGTGTACTGA AGGTGGGCAT CACGGCTTTC TATCAGATAG TGTCGTTTGT GTGTGAGGAC 
ACACAGAGAC ATCCACCAAC CCGCCAGTTC TTCACATCCT GCATTGAGAT CCTCGGCCAG 
GTCTTTATTC GGGACGTCCA ATCAGAATGT AAACACATGC TAAAGACTAT CCTACTCAAC 
AGACGTCTGT GTAACCTACT GTCACCATAC TTCACCCCGA ACGCCTCTCC CTCAGAGCTG 
GTCTCTCTGT ATGAAGAGGT GGTCAACACC CTCCACATCG ACAGCGGTGA TGTCATATTC 
ATGCTCCTCA CAAAGTTTGA CTTGTTTCAG TGGTTGTCGA CGACCCAGCC TCCGTTCTCT 
GAGCGTAGTC GTCTGATGAC CGTCATCCAT CAGGCTCTCT GCACATGTGG GCTGGAGCCT 
GAGCCCGAAG TCCTCATGCC ATTCAACATC TTCTGCAAGC ACTGGACTGA ACTGCTCCTC 
TACCAGTTTC CTGACCACTA CAGCGACTTC CTCCGTCTCC TCATGCAGAG CTCTTCCGAT 
CAGCTTCTTA GTCCTGACTG TTGGAGAACG TCACTGAAAG CTCTGGGCTG TTCTCCTCAA 
ACTAAGAATA AGACAAACAA ATCCCGCTCC ATGCTCCTCT CCCCTCAACA GGTGGATGAA 
ACAATCGAGT GGCTCTCAAA GTACTTCCTA AAGCTCCGAC TGTCTAACGG GGACTTCAGG 
AGCTTTGGTC TTCTTTCAAA ATGGGGGCCA TACATTGAGG AAGTCAAGGT GTTTTGGGAA 
GATCTGATTA CCTACGTCAT TGATCTGGAA CTAAACCATT GCGGCAAAGA ACCTGTAGGA 
AGTCCAAGAC TGCTCAAAGC TCTGGAGAAC CTGCATAGAA AAATGGCCAA ACTCTTTGAG 
CCGTGGATTT TGGTGCTTGA CACTGACAAC ACTAGTCATC CTCGCTGTTC TCCATGGCTT 
GAATCAGACG CCGCAGTTGC AGCGAGCTTA GTAGGCTTAT ATACACGCTT GACAGAGACT 
TTACATGAGA AATTCAAAGA TCGCTTGTTG CCAGGACAGA GAGGAGCTCT GTGGCTTCAT 
CTCATGTTTT ACTGTGAGAC CTGCACTTCT CCTAAAATGC CAGAGTATCT GCTGTACACC 
TACCACACTG AGTACAGCCG TTTGCCCTGG AAGGACCTGC ATCCAGATCA GACACTAATG 
AACCAGTTTT TCAATGTGGA GAGGGGAAGT CCAAAGAGTT GTTTTGTGTT CATGGGAGAG 
TTGCTGTGTG AGGTGAACTG GGTTAGTGTA TTGAGTGACT CTCTCAGCCC CCAGCTGAAC 
CCCTCAACCC ACACAATGGT GGTCTCCGTA CTCCACCTCA TGGTGTTTCT GGCCAAAGAG 
GATCTCAGTA AACCCGAGTC CTCTGTTCTC AGTCTGCTGG ACCAGTCAGC TTCTCTGCCT 
TGGAATCTGG TGGATTTTTC TTCATATCAA AATGTCACGC GCTATGTCAG CACACATTAC 
CCAGCGTCCC TTGTTCTCAG TCACGACACT TCTTCACAGC AGGTCGTCAG ATTGCTCAGA 
ATGGCTGCTG GATTTGAGCC GACCACACAA GTGTTGCATC ACAAGGACAT GACTCTAAAG 
TGTCAAGCCT TCCTTCACCT CATGGTGCAG TTCCTGACCT CGCTGGATCA AAATGGGAAC 
ATCAGTCTGG CATCGCTAGA GACAGAAATG GAGAAATTAC TGGAGAACAT AGTCATTTTC 
AACCCACCTG AGACGGACCT CCAGCAGCGA CACATGGCCA CCTGCAGTCT GTTTGCAGAG 
ATGCTGACTC TGCTGAATGA GGCCGGTATC TCGACAGCAG AGGGTCTGGG CACTAAACTG 
CACTGCTGGG TGGAGAAGCG GGCTCGAGGG CCTCTGCTTC TGCCTTTGCT AACGGCTGCG 
TGCAGATGCC TGGCTTCAGT GCGTCACATG ACACGCACCA CCGAGGCCTG TATTCTCTCC 
TACTTCACCG ATGGAGGATG TGTGGATCAG TACTCTGGAT GGGGGCCCAT CCTGGTGTCT 
CTTCAGGTTC CTGAACTGAC TGTTGAGGAC TTCATTCAGG AAAGCCTAAG TCTGGGCAGC 
TTCCTCACGC TGTACGTCTA CATCCTCCAG AGGCTAAACC TGGAGCAAAC CCTCCTCAAT 
GAGAAGAAAA CCTTAATGCT GCTCAATACG TGGATCAGTC AGGTGTTTCC CAGTGGTCCA 
GCTGATGAGG CCAAGCTGTT TTTATGGTGG CATAAATTCT TGGAGCTTCT GCAAATCCAA 
GTGGACCAAG AGGATGCCTG CTCACTGGAC ACTCTCATTC TCACCTTAAT GGCGTTTCAG 
AATCGTCTCG TTCAACTCGG AGAGGAAAGA CTAAATTCCG GCATCCTGGG AGCCATTGGC 
CTTGGGAGGA AATCACCACT TTCTAACAGG TTTCGGGTTG TGGCGCGCAG CATGTCAGCC 
TTTCTGCTGG TTCAGGTGCC CTCAGAGAAT CAGCTGCGTC TTCATCCCGG GACAGAGCTG 
CAGCTGTCAG CTAAAGCCCA GCAGGCCTTG TCTGCACTGG AGGCGATGGC CAGTAATAAG 
CAGTATTCAG AGCTGCATGT GTCTGTGAAC CAGGCCTGTC AGTTCATCAA GTACCCGAAT 
CACTGTCTGC GAGACGCCAA CACTCTTCTC ACTCTGCTGG TCAATGCACT CTACACTGAC 
CTTCATTACC TGGACATCAT CCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165418.1
CDS	193..7839
Translation	MEAVRPKKAKSKSSGKPQVKKERQSESVRQPSGSSKAVNEPEFTDVPLSLPHVPPDLNTAASQTSLDHHDKAENEQQHVLLCEKPEVVQKSTCHEDKEHELPSQDAAPRLDQTSVLAPSSQSDPKPLSAKLEPFSVPSIYPSIPASLSQPQLSEDSYSISYELLDGSLMPAASERLVAPAVLTLANQESSPPSLVPVEVVDVERSRERLYPELPKTCQSVKPFTSEQLRMWEPGSWLENVELHEAEFQNLAHQEGHELYELLLSYWRCRKQLTQAQTELQAANSDCKNVQNRLWSFKDERLSLQGICADQNKVYGYHCYQQVTLNEAALAELKHLFSAKAEILHQTVALHSYTSILSRLQVESYLYRLLSSNPTTKSVAVQDTSAVSPKSQPSSLQPLKESISVLFIFTRRVLDDSQFQADIHLWLQRLVSVLHQVGSTGDHLFILNHLLCCPAGVGKWAVPFLQIKVLDNPSGVYHFMQALAILMSPARHRPDFLGHMKPSDARGNNSLTEPASGNWTLVDEGGEEDEDPETSWMLLSEDDLVSLFSQFPFDELFKHLLGICSKGDYQPLATTSQKMMKIFAFASSLVELLAVGLQTYNRARYRQFVKRIGHMIRMTLCYVSDHWAQYISLTGVSRTTVQEQSFSMEKLQVEFDHLFLRAVLHVLKAKRLGIWLFMSEMPYGTLSSSMLWKIFYIMQCAETDGLEKLSCSLSAEDCIQRLRESSHQERFQLWLSEINSSDGICLLTTFAHMAQPRRTDVDPEFVRNIVLEIYEVSYVSVTTREIFSKVGRELLAAIAAAHPHIISVLLERLRETIERVGMVSLYLFKELPLHLWMPAQEEVGLIRDWLMNFSLTAVENRLACIILEGLNWGFQPNGCLILPAPLHSDVALLVIEAYQKYLTDKPYGGILSEGIKQVSYLANVVRLGQTPESSFNQWAWDIVLRLKLHANERSPQDAWCPLPSSSAPSVPEITDSPIMHPVFKAVKAGIPIGCFLAIEMTTIGHSLEKFCSEGISLLKTLIQSRHLRAAVHILENILPLVYNCQFYLLKNEQFLSCVQLFLQLDSGTQGVTQQVTQKVAQHLIGPATCENIKLLNSVIQAHVLESSRNGRVGAVAVLEFWLQVLTEQSLWYRDRAVLFLLDHMCRSAFLHQQEECLQKILYQQHKSALGYHGDKGLLSSIMGWIVAGNVTPSFIEGSSLTGEVWFAWMILNMETMFEEDSQLRRCVEHELLSNPACTPDQALKKAQVRLKLQVVPSLQRLMIYRWAQQALATPADHPILPLVWQKFFQLYLRQPGPEFGLEVKGCIGRRYFHSAAHLSLLKSLRQRLVEVSDFHHAASKALKVPCASGDSGDGLLTPGTPITQYMTSPDLHTEIVRLFTVFALWLDDENLQKQEIYLPSLPKQYDTHRLAKIMQRQQYFEVWMEHVDIERVQHELQEVLDLWSKVKSEPAVSQNSSSSIFTDFINPLAARERILTHLRKHDAPQGSLLPVPMKAPVPDIPTASLTDEKISTTLIQEDLTKLQHQAKLATVRETQQVALDNELLEVLPQLYINREDQLSMQLECRGKSGQPCQGPANITVKYSSMHKLEPVQNQIQSLKSDIKQLQADGIKAPPQSLAEAAVHTENFITALVNVFKQNPSPGVLKVGITAFYQIVSFVCEDTQRHPPTRQFFTSCIEILGQVFIRDVQSECKHMLKTILLNRRLCNLLSPYFTPNASPSELVSLYEEVVNTLHIDSGDVIFMLLTKFDLFQWLSTTQPPFSERSRLMTVIHQALCTCGLEPEPEVLMPFNIFCKHWTELLLYQFPDHYSDFLRLLMQSSSDQLLSPDCWRTSLKALGCSPQTKNKTNKSRSMLLSPQQVDETIEWLSKYFLKLRLSNGDFRSFGLLSKWGPYIEEVKVFWEDLITYVIDLELNHCGKEPVGSPRLLKALENLHRKMAKLFEPWILVLDTDNTSHPRCSPWLESDAAVAASLVGLYTRLTETLHEKFKDRLLPGQRGALWLHLMFYCETCTSPKMPEYLLYTYHTEYSRLPWKDLHPDQTLMNQFFNVERGSPKSCFVFMGELLCEVNWVSVLSDSLSPQLNPSTHTMVVSVLHLMVFLAKEDLSKPESSVLSLLDQSASLPWNLVDFSSYQNVTRYVSTHYPASLVLSHDTSSQQVVRLLRMAAGFEPTTQVLHHKDMTLKCQAFLHLMVQFLTSLDQNGNISLASLETEMEKLLENIVIFNPPETDLQQRHMATCSLFAEMLTLLNEAGISTAEGLGTKLHCWVEKRARGPLLLPLLTAACRCLASVRHMTRTTEACILSYFTDGGCVDQYSGWGPILVSLQVPELTVEDFIQESLSLGSFLTLYVYILQRLNLEQTLLNEKKTLMLLNTWISQVFPSGPADEAKLFLWWHKFLELLQIQVDQEDACSLDTLILTLMAFQNRLVQLGEERLNSGILGAIGLGRKSPLSNRFRVVARSMSAFLLVQVPSENQLRLHPGTELQLSAKAQQALSALEAMASNKQYSELHVSVNQACQFIKYPNHCLRDANTLLTLLVNALYTDLHYLDIIR

Target ORF information:

RefSeq Version	XM_005165361.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ectopic P-granules autophagy protein 5 homolog (C. elegans) (epg5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165361.4

ORF Insert Sequence:

ATGGAGGCTG TGAGACCTAA AAAGGCAAAG TCCAAATCCA GTGGGAAGCC ACAGGTCAAA 
AAGGAGAGGC AGTCAGAGAG CGTCAGACAG CCCTCGGGAT CTTCTAAAGC AGTTAATGAG 
CCTGAGTTCA CTGATGTGCC GCTCAGCCTC CCTCATGTGC CTCCTGACCT CAACACAGCT 
GCCTCTCAGA CTTCACTAGA TCATCATGAC AAAGCTGAAA ATGAGCAACA GCATGTTCTC 
TTATGCGAGA AGCCTGAAGT AGTACAGAAA TCGACGTGTC ATGAAGACAA AGAGCATGAA 
TTGCCATCAC AAGATGCTGC ACCTCGGCTG GACCAAACAT CAGTGCTTGC TCCGTCCAGC 
CAAAGTGATC CCAAACCCCT CAGTGCTAAA CTTGAGCCAT TTAGTGTCCC CTCTATATAT 
CCCTCTATCC CAGCTTCCCT CTCCCAGCCT CAGCTTTCAG AGGACTCGTA CTCTATCTCC 
TACGAACTGT TAGATGGCTC TTTAATGCCA GCAGCGTCTG AGAGACTAGT AGCTCCGGCT 
GTTTTGACTC TGGCTAACCA GGAGTCCTCT CCGCCCAGCT TGGTTCCAGT GGAGGTTGTG 
GATGTGGAAA GATCTAGAGA GAGGCTTTAC CCAGAGCTGC CTAAGACCTG CCAGTCTGTG 
AAGCCCTTCA CTAGTGAGCA GCTGCGCATG TGGGAGCCCG GTTCCTGGCT GGAAAACGTG 
GAGCTGCATG AAGCCGAATT CCAGAATCTG GCTCATCAAG AGGGACATGA GCTGTACGAG 
CTGCTGCTGA GCTACTGGAG GTGCCGGAAG CAGCTGACGC AGGCCCAGAC TGAACTGCAG 
GCTGCCAACT CGGACTGCAA AAATGTGCAG AATCGCCTGT GGAGCTTCAA GGATGAAAGG 
CTTTCGTTAC AGGGTATATG TGCAGACCAG AATAAGGTGT ATGGCTACCA CTGCTACCAG 
CAGGTGACAC TGAACGAGGC TGCGTTAGCT GAGCTGAAGC ACCTGTTTTC TGCTAAAGCG 
GAAATCCTCC ATCAGACAGT GGCTCTGCAC TCATACACCT CCATCCTGTC CCGTCTGCAA 
GTGGAGTCTT ATCTCTACCG CTTGCTCAGC AGTAACCCCA CCACCAAGAG TGTGGCTGTG 
CAAGATACAA GTGCAGTGAG TCCAAAGTCT CAGCCCTCGT CTTTGCAGCC GCTGAAGGAA 
AGCATCAGTG TGCTGTTCAT CTTCACCCGA CGGGTCCTAG ATGATTCTCA GTTTCAGGCT 
GACATCCATC TTTGGCTTCA GAGGCTGGTG TCTGTGCTGC ATCAGGTCGG TTCTACAGGA 
GATCATCTAT TCATCCTAAA CCACCTGCTC TGCTGCCCGG CAGGTGTAGG GAAATGGGCT 
GTGCCATTCC TACAGATCAA AGTATTGGAT AACCCATCTG GAGTATATCA TTTCATGCAG 
GCCCTTGCTA TCCTTATGTC TCCAGCAAGG CACCGTCCAG ACTTCCTGGG ACACATGAAG 
CCCAGTGATG CAAGGGGCAA CAACAGCCTA ACAGAACCTG CATCTGGTAA CTGGACTCTA 
GTGGATGAAG GTGGAGAAGA GGACGAAGAC CCAGAAACGA GCTGGATGCT GCTGTCTGAG 
GATGATCTGG TTTCTCTGTT CTCTCAGTTT CCCTTTGATG AGCTCTTCAA ACATTTACTG 
GGCATCTGCT CTAAAGGGGA CTATCAGCCT CTAGCCACCA CAAGTCAAAA GATGATGAAG 
ATATTTGCGT TTGCCTCTTC ACTGGTTGAG TTGCTAGCAG TCGGCTTGCA GACCTACAAC 
AGAGCACGCT ATCGGCAGTT TGTGAAGAGG ATTGGTCACA TGATTCGGAT GACGCTCTGC 
TATGTGAGTG ACCACTGGGC GCAGTACATC AGTCTGACCG GTGTGAGCAG GACCACAGTG 
CAGGAGCAGT CATTTTCAAT GGAGAAACTG CAGGTGGAGT TTGACCATCT CTTCCTGAGG 
GCGGTGCTGC ATGTGCTTAA GGCCAAGAGA CTGGGCATAT GGCTGTTCAT GTCTGAGATG 
CCGTATGGGA CATTGTCCAG CTCCATGCTG TGGAAGATCT TCTACATTAT GCAGTGTGCA 
GAGACAGATG GTCTGGAGAA GCTCAGTTGT AGCTTGAGTG CTGAGGACTG TATCCAGAGG 
CTCAGAGAAT CAAGCCATCA GGAGAGGTTT CAGCTTTGGC TGTCTGAGAT AAACAGCTCA 
GATGGGATCT GCCTGTTGAC AACGTTTGCG CACATGGCCC AGCCGAGACG CACCGATGTA 
GACCCTGAGT TTGTCAGGAA CATAGTGCTA GAGATATACG AGGTTTCCTA TGTAAGTGTC 
ACCACCCGTG AGATCTTCTC TAAAGTTGGA CGGGAATTGC TTGCAGCGAT AGCGGCTGCC 
CACCCTCACA TCATCTCTGT GTTGCTGGAG AGACTGAGAG AGACCATAGA GAGAGTGGGC 
ATGGTGTCGT TGTACCTGTT TAAAGAGTTG CCCTTGCACT TGTGGATGCC AGCTCAGGAG 
GAGGTGGGGC TGATCAGAGA CTGGCTGATG AACTTCAGTC TCACTGCTGT TGAGAACAGA 
TTAGCCTGCA TTATACTAGA GGGCCTCAAC TGGGGATTTC AGCCAAATGG TTGCCTTATT 
TTACCTGCAC CTCTGCACAG TGACGTTGCC TTGTTGGTGA TTGAGGCATA TCAAAAATAT 
CTTACAGATA AACCATATGG TGGCATCCTC TCAGAAGGCA TTAAACAGGT GTCTTACCTG 
GCTAACGTGG TGAGGCTCGG CCAGACTCCA GAATCTTCCT TTAATCAGTG GGCTTGGGAT 
ATAGTTCTGA GGCTAAAGCT CCACGCCAAT GAAAGAAGCC CACAGGATGC ATGGTGCCCG 
CTTCCATCCT CCAGTGCCCC CAGTGTGCCA GAGATCACAG ACTCTCCCAT TATGCACCCA 
GTGTTCAAAG CGGTCAAAGC CGGCATCCCC ATTGGCTGTT TTCTGGCTAT TGAGATGACC 
ACCATCGGGC ACAGTTTGGA GAAGTTTTGC AGTGAAGGAA TATCTTTGCT GAAGACTCTG 
ATTCAGTCCC GTCATCTGAG AGCTGCTGTG CACATACTGG AGAACATTCT TCCTCTGGTC 
TACAACTGCC AGTTCTACCT GCTTAAGAAT GAGCAGTTTC TGAGCTGTGT CCAGCTGTTT 
TTGCAGCTGG ATAGTGGCAC TCAAGGCGTA ACCCAGCAGG TCACACAGAA GGTGGCCCAG 
CATCTGATTG GCCCCGCTAC ATGTGAGAAT ATCAAACTGC TGAACAGTGT GATCCAGGCT 
CATGTTCTGG AGAGCTCCAG GAATGGACGT GTTGGTGCAG TCGCTGTTCT GGAGTTCTGG 
CTTCAGGTTT TGACTGAACA GAGCCTCTGG TATCGAGACA GAGCTGTTCT CTTTCTTCTG 
GACCACATGT GCCGTTCTGC ATTTCTTCAC CAACAAGAGG AATGTCTCCA GAAAATACTC 
TACCAGCAGC ACAAGAGCGC TTTAGGTTAC CATGGAGACA AAGGTTTGCT CTCGTCTATT 
ATGGGCTGGA TAGTGGCAGG AAATGTTACT CCGTCTTTTA TTGAGGGCAG TTCTCTCACA 
GGAGAGGTGT GGTTTGCTTG GATGATCCTA AACATGGAGA CAATGTTCGA GGAAGATTCC 
CAGCTGCGAC GCTGTGTAGA GCATGAGCTG CTCTCAAACC CTGCGTGTAC ACCAGATCAG 
GCTTTGAAGA AAGCCCAGGT GCGTCTCAAG CTTCAGGTTG TCCCATCACT GCAGAGGCTG 
ATGATCTATC GCTGGGCTCA ACAAGCTTTA GCCACTCCTG CAGACCATCC AATCCTGCCT 
TTGGTGTGGC AGAAATTCTT CCAGCTTTAC CTCCGTCAAC CTGGACCAGA GTTTGGGCTG 
GAGGTGAAAG GTTGCATTGG CAGGCGTTAT TTCCATAGTG CGGCCCATCT GTCCTTGCTG 
AAGAGCCTCA GACAGCGGCT GGTGGAAGTG TCTGATTTCC ACCACGCCGC CAGCAAGGCT 
CTGAAAGTGC CCTGCGCTAG TGGAGATTCC GGTGATGGTC TGCTGACCCC CGGGACGCCC 
ATCACACAGT ACATGACCTC TCCTGACCTG CACACTGAGA TCGTGCGGCT CTTCACTGTG 
TTTGCCTTGT GGTTGGATGA TGAAAACCTT CAGAAGCAGG AAATTTACTT GCCCTCTTTG 
CCAAAGCAGT ATGACACCCA TCGCCTGGCC AAAATTATGC AGCGCCAGCA GTATTTTGAG 
GTGTGGATGG AGCATGTGGA TATCGAGCGA GTCCAGCACG AGCTGCAGGA AGTGCTCGAT 
CTGTGGTCAA AGGTCAAGAG TGAACCTGCA GTCTCACAGA ACAGCAGCTC CTCCATCTTT 
ACCGACTTCA TCAACCCTCT GGCTGCAAGG GAGCGTATTC TCACTCACCT GAGAAAGCAT 
GATGCCCCTC AGGGGTCGCT GCTGCCAGTG CCCATGAAAG CTCCAGTGCC TGATATTCCC 
ACTGCGAGCC TAACTGATGA GAAAATCTCC ACCACCTTAA TTCAAGAGGA CCTCACTAAG 
CTCCAGCATC AAGCCAAGTT GGCAACGGTT CGGGAAACCC AGCAGGTTGC TCTAGACAAT 
GAACTGTTGG AGGTGTTGCC ACAGCTCTAC ATAAACCGGG AAGACCAGCT GTCAATGCAG 
TTGGAGTGCC GTGGAAAAAG TGGACAGCCC TGCCAAGGTC CTGCAAATAT CACTGTTAAG 
TACTCAAGTA TGCACAAACT GGAGCCGGTG CAGAATCAGA TCCAGTCTCT GAAGAGTGAC 
ATTAAACAGC TGCAGGCCGA TGGAATCAAA GCTCCTCCTC AGAGTCTGGC TGAGGCGGCC 
GTTCACACTG AGAACTTCAT CACGGCCTTG GTTAATGTTT TCAAGCAAAA TCCATCCCCT 
GGTGTACTGA AGGTGGGCAT CACGGCTTTC TATCAGATAG TGTCGTTTGT GTGTGAGGAC 
ACACAGAGAC ATCCACCAAC CCGCCAGTTC TTCACATCCT GCATTGAGAT CCTCGGCCAG 
GTCTTTATTC GGGACGTCCA ATCAGAATGT AAACACATGC TAAAGACTAT CCTACTCAAC 
AGACGTCTGT GTAACCTACT GTCACCATAC TTCACCCCGA ACGCCTCTCC CTCAGAGCTG 
GTCTCTCTGT ATGAAGAGGT GGTCAACACC CTCCACATCG ACAGCGGTGA TGTCATATTC 
ATGCTCCTCA CAAAGTTTGA CTTGTTTCAG TGGTTGTCGA CGACCCAGCC TCCGTTCTCT 
GAGCGTAGTC GTCTGATGAC CGTCATCCAT CAGGCTCTCT GCACATGTGG GCTGGAGCCT 
GAGCCCGAAG TCCTCATGCC ATTCAACATC TTCTGCAAGC ACTGGACTGA ACTGCTCCTC 
TACCAGTTTC CTGACCACTA CAGCGACTTC CTCCGTCTCC TCATGCAGAG CTCTTCCGAT 
CAGCTTCTTA GTCCTGACTG TTGGAGAACG TCACTGAAAG CTCTGGGCTG TTCTCCTCAA 
ACTAAGAATA AGACAAACAA ATCCCGCTCC ATGCTCCTCT CCCCTCAACA GGTGGATGAA 
ACAATCGAGT GGCTCTCAAA GTACTTCCTA AAGCTCCGAC TGTCTAACGG GGACTTCAGG 
AGCTTTGGTC TTCTTTCAAA ATGGGGGCCA TACATTGAGG AAGTCAAGGT GTTTTGGGAA 
GATCTGATTA CCTACGTCAT TGATCTGGAA CTAAACCATT GCGGCAAAGA ACCTGTAGGA 
AGTCCAAGAC TGCTCAAAGC TCTGGAGAAC CTGCATAGAA AAATGGCCAA ACTCTTTGAG 
CCGTGGATTT TGGTGCTTGA CACTGACAAC ACTAGTCATC CTCGCTGTTC TCCATGGCTT 
GAATCAGACG CCGCAGTTGC AGCGAGCTTA GTAGGCTTAT ATACACGCTT GACAGAGACT 
TTACATGAGA AATTCAAAGA TCGCTTGTTG CCAGGACAGA GAGGAGCTCT GTGGCTTCAT 
CTCATGTTTT ACTGTGAGAC CTGCACTTCT CCTAAAATGC CAGAGTATCT GCTGTACACC 
TACCACACTG AGTACAGCCG TTTGCCCTGG AAGGACCTGC ATCCAGATCA GACACTAATG 
AACCAGTTTT TCAATGTGGA GAGGGGAAGT CCAAAGAGTT GTTTTGTGTT CATGGGAGAG 
TTGCTGTGTG AGGTGAACTG GGTTAGTGTA TTGAGTGACT CTCTCAGCCC CCAGCTGAAC 
CCCTCAACCC ACACAATGGT GGTCTCCGTA CTCCACCTCA TGGTGTTTCT GGCCAAAGAG 
GATCTCAGTA AACCCGAGTC CTCTGTTCTC AGTCTGCTGG ACCAGTCAGC TTCTCTGCCT 
TGGAATCTGG TGGATTTTTC TTCATATCAA AATGTCACGC GCTATGTCAG CACACATTAC 
CCAGCGTCCC TTGTTCTCAG TCACGACACT TCTTCACAGC AGGTCGTCAG ATTGCTCAGA 
ATGGCTGCTG GATTTGAGCC GACCACACAA GTGTTGCATC ACAAGGACAT GACTCTAAAG 
TGTCAAGCCT TCCTTCACCT CATGGTGCAG TTCCTGACCT CGCTGGATCA AAATGGGAAC 
ATCAGTCTGG CATCGCTAGA GACAGAAATG GAGAAATTAC TGGAGAACAT AGTCATTTTC 
AACCCACCTG AGACGGACCT CCAGCAGCGA CACATGGCCA CCTGCAGTCT GTTTGCAGAG 
ATGCTGACTC TGCTGAATGA GGCCGGTATC TCGACAGCAG AGGGTCTGGG CACTAAACTG 
CACTGCTGGG TGGAGAAGCG GGCTCGAGGG CCTCTGCTTC TGCCTTTGCT AACGGCTGCG 
TGCAGATGCC TGGCTTCAGT GCGTCACATG ACACGCACCA CCGAGGCCTG TATTCTCTCC 
TACTTCACCG ATGGAGGATG TGTGGATCAG TACTCTGGAT GGGGGCCCAT CCTGGTGTCT 
CTTCAGGTTC CTGAACTGAC TGTTGAGGAC TTCATTCAGG AAAGCCTAAG TCTGGGCAGC 
TTCCTCACGC TGTACGTCTA CATCCTCCAG AGGCTAAACC TGGAGCAAAC CCTCCTCAAT 
GAGAAGAAAA CCTTAATGCT GCTCAATACG TGGATCAGTC AGGTGTTTCC CAGTGGTCCA 
GCTGATGAGG CCAAGCTGTT TTTATGGTGG CATAAATTCT TGGAGCTTCT GCAAATCCAA 
GTGGACCAAG AGGATGCCTG CTCACTGGAC ACTCTCATTC TCACCTTAAT GGCGTTTCAG 
AATCGTCTCG TTCAACTCGG AGAGGAAAGA CTAAATTCCG GCATCCTGGG AGCCATTGGC 
CTTGGGAGGA AATCACCACT TTCTAACAGG TTTCGGGTTG TGGCGCGCAG CATGTCAGCC 
TTTCTGCTGG TTCAGGTGCC CTCAGAGAAT CAGCTGCGTC TTCATCCCGG GACAGAGCTG 
CAGCTGTCAG CTAAAGCCCA GCAGGCCTTG TCTGCACTGG AGGCGATGGC CAGTAATAAG 
CAGTATTCAG AGCTGCATGT GTCTGTGAAC CAGGCCTGTC AGTTCATCAA GTACCCGAAT 
CACTGTCTGC GAGACGCCAA CACTCTTCTC ACTCTGCTGG TCAATGCACT CTACACTGAC 
CTTCATTACC TGGACATCAT CCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EPG5 ( XM_005165361.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005165418.1 Danio rerio ectopic P-granules autophagy protein 5 homolog (C. elegans) (epg5), transcript variant X1, mRNA.

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CloneID	ODa10746
Clone ID Related Accession (Same CDS sequence)	XM_005165361.4 , XM_005165361.3
Accession Version	XM_005165361.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ectopic P granules protein 5 homolog isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165361.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG TGAGACCTAA AAAGGCAAAG TCCAAATCCA GTGGGAAGCC ACAGGTCAAA 
AAGGAGAGGC AGTCAGAGAG CGTCAGACAG CCCTCGGGAT CTTCTAAAGC AGTTAATGAG 
CCTGAGTTCA CTGATGTGCC GCTCAGCCTC CCTCATGTGC CTCCTGACCT CAACACAGCT 
GCCTCTCAGA CTTCACTAGA TCATCATGAC AAAGCTGAAA ATGAGCAACA GCATGTTCTC 
TTATGCGAGA AGCCTGAAGT AGTACAGAAA TCGACGTGTC ATGAAGACAA AGAGCATGAA 
TTGCCATCAC AAGATGCTGC ACCTCGGCTG GACCAAACAT CAGTGCTTGC TCCGTCCAGC 
CAAAGTGATC CCAAACCCCT CAGTGCTAAA CTTGAGCCAT TTAGTGTCCC CTCTATATAT 
CCCTCTATCC CAGCTTCCCT CTCCCAGCCT CAGCTTTCAG AGGACTCGTA CTCTATCTCC 
TACGAACTGT TAGATGGCTC TTTAATGCCA GCAGCGTCTG AGAGACTAGT AGCTCCGGCT 
GTTTTGACTC TGGCTAACCA GGAGTCCTCT CCGCCCAGCT TGGTTCCAGT GGAGGTTGTG 
GATGTGGAAA GATCTAGAGA GAGGCTTTAC CCAGAGCTGC CTAAGACCTG CCAGTCTGTG 
AAGCCCTTCA CTAGTGAGCA GCTGCGCATG TGGGAGCCCG GTTCCTGGCT GGAAAACGTG 
GAGCTGCATG AAGCCGAATT CCAGAATCTG GCTCATCAAG AGGGACATGA GCTGTACGAG 
CTGCTGCTGA GCTACTGGAG GTGCCGGAAG CAGCTGACGC AGGCCCAGAC TGAACTGCAG 
GCTGCCAACT CGGACTGCAA AAATGTGCAG AATCGCCTGT GGAGCTTCAA GGATGAAAGG 
CTTTCGTTAC AGGGTATATG TGCAGACCAG AATAAGGTGT ATGGCTACCA CTGCTACCAG 
CAGGTGACAC TGAACGAGGC TGCGTTAGCT GAGCTGAAGC ACCTGTTTTC TGCTAAAGCG 
GAAATCCTCC ATCAGACAGT GGCTCTGCAC TCATACACCT CCATCCTGTC CCGTCTGCAA 
GTGGAGTCTT ATCTCTACCG CTTGCTCAGC AGTAACCCCA CCACCAAGAG TGTGGCTGTG 
CAAGATACAA GTGCAGTGAG TCCAAAGTCT CAGCCCTCGT CTTTGCAGCC GCTGAAGGAA 
AGCATCAGTG TGCTGTTCAT CTTCACCCGA CGGGTCCTAG ATGATTCTCA GTTTCAGGCT 
GACATCCATC TTTGGCTTCA GAGGCTGGTG TCTGTGCTGC ATCAGGTCGG TTCTACAGGA 
GATCATCTAT TCATCCTAAA CCACCTGCTC TGCTGCCCGG CAGGTGTAGG GAAATGGGCT 
GTGCCATTCC TACAGATCAA AGTATTGGAT AACCCATCTG GAGTATATCA TTTCATGCAG 
GCCCTTGCTA TCCTTATGTC TCCAGCAAGG CACCGTCCAG ACTTCCTGGG ACACATGAAG 
CCCAGTGATG CAAGGGGCAA CAACAGCCTA ACAGAACCTG CATCTGGTAA CTGGACTCTA 
GTGGATGAAG GTGGAGAAGA GGACGAAGAC CCAGAAACGA GCTGGATGCT GCTGTCTGAG 
GATGATCTGG TTTCTCTGTT CTCTCAGTTT CCCTTTGATG AGCTCTTCAA ACATTTACTG 
GGCATCTGCT CTAAAGGGGA CTATCAGCCT CTAGCCACCA CAAGTCAAAA GATGATGAAG 
ATATTTGCGT TTGCCTCTTC ACTGGTTGAG TTGCTAGCAG TCGGCTTGCA GACCTACAAC 
AGAGCACGCT ATCGGCAGTT TGTGAAGAGG ATTGGTCACA TGATTCGGAT GACGCTCTGC 
TATGTGAGTG ACCACTGGGC GCAGTACATC AGTCTGACCG GTGTGAGCAG GACCACAGTG 
CAGGAGCAGT CATTTTCAAT GGAGAAACTG CAGGTGGAGT TTGACCATCT CTTCCTGAGG 
GCGGTGCTGC ATGTGCTTAA GGCCAAGAGA CTGGGCATAT GGCTGTTCAT GTCTGAGATG 
CCGTATGGGA CATTGTCCAG CTCCATGCTG TGGAAGATCT TCTACATTAT GCAGTGTGCA 
GAGACAGATG GTCTGGAGAA GCTCAGTTGT AGCTTGAGTG CTGAGGACTG TATCCAGAGG 
CTCAGAGAAT CAAGCCATCA GGAGAGGTTT CAGCTTTGGC TGTCTGAGAT AAACAGCTCA 
GATGGGATCT GCCTGTTGAC AACGTTTGCG CACATGGCCC AGCCGAGACG CACCGATGTA 
GACCCTGAGT TTGTCAGGAA CATAGTGCTA GAGATATACG AGGTTTCCTA TGTAAGTGTC 
ACCACCCGTG AGATCTTCTC TAAAGTTGGA CGGGAATTGC TTGCAGCGAT AGCGGCTGCC 
CACCCTCACA TCATCTCTGT GTTGCTGGAG AGACTGAGAG AGACCATAGA GAGAGTGGGC 
ATGGTGTCGT TGTACCTGTT TAAAGAGTTG CCCTTGCACT TGTGGATGCC AGCTCAGGAG 
GAGGTGGGGC TGATCAGAGA CTGGCTGATG AACTTCAGTC TCACTGCTGT TGAGAACAGA 
TTAGCCTGCA TTATACTAGA GGGCCTCAAC TGGGGATTTC AGCCAAATGG TTGCCTTATT 
TTACCTGCAC CTCTGCACAG TGACGTTGCC TTGTTGGTGA TTGAGGCATA TCAAAAATAT 
CTTACAGATA AACCATATGG TGGCATCCTC TCAGAAGGCA TTAAACAGGT GTCTTACCTG 
GCTAACGTGG TGAGGCTCGG CCAGACTCCA GAATCTTCCT TTAATCAGTG GGCTTGGGAT 
ATAGTTCTGA GGCTAAAGCT CCACGCCAAT GAAAGAAGCC CACAGGATGC ATGGTGCCCG 
CTTCCATCCT CCAGTGCCCC CAGTGTGCCA GAGATCACAG ACTCTCCCAT TATGCACCCA 
GTGTTCAAAG CGGTCAAAGC CGGCATCCCC ATTGGCTGTT TTCTGGCTAT TGAGATGACC 
ACCATCGGGC ACAGTTTGGA GAAGTTTTGC AGTGAAGGAA TATCTTTGCT GAAGACTCTG 
ATTCAGTCCC GTCATCTGAG AGCTGCTGTG CACATACTGG AGAACATTCT TCCTCTGGTC 
TACAACTGCC AGTTCTACCT GCTTAAGAAT GAGCAGTTTC TGAGCTGTGT CCAGCTGTTT 
TTGCAGCTGG ATAGTGGCAC TCAAGGCGTA ACCCAGCAGG TCACACAGAA GGTGGCCCAG 
CATCTGATTG GCCCCGCTAC ATGTGAGAAT ATCAAACTGC TGAACAGTGT GATCCAGGCT 
CATGTTCTGG AGAGCTCCAG GAATGGACGT GTTGGTGCAG TCGCTGTTCT GGAGTTCTGG 
CTTCAGGTTT TGACTGAACA GAGCCTCTGG TATCGAGACA GAGCTGTTCT CTTTCTTCTG 
GACCACATGT GCCGTTCTGC ATTTCTTCAC CAACAAGAGG AATGTCTCCA GAAAATACTC 
TACCAGCAGC ACAAGAGCGC TTTAGGTTAC CATGGAGACA AAGGTTTGCT CTCGTCTATT 
ATGGGCTGGA TAGTGGCAGG AAATGTTACT CCGTCTTTTA TTGAGGGCAG TTCTCTCACA 
GGAGAGGTGT GGTTTGCTTG GATGATCCTA AACATGGAGA CAATGTTCGA GGAAGATTCC 
CAGCTGCGAC GCTGTGTAGA GCATGAGCTG CTCTCAAACC CTGCGTGTAC ACCAGATCAG 
GCTTTGAAGA AAGCCCAGGT GCGTCTCAAG CTTCAGGTTG TCCCATCACT GCAGAGGCTG 
ATGATCTATC GCTGGGCTCA ACAAGCTTTA GCCACTCCTG CAGACCATCC AATCCTGCCT 
TTGGTGTGGC AGAAATTCTT CCAGCTTTAC CTCCGTCAAC CTGGACCAGA GTTTGGGCTG 
GAGGTGAAAG GTTGCATTGG CAGGCGTTAT TTCCATAGTG CGGCCCATCT GTCCTTGCTG 
AAGAGCCTCA GACAGCGGCT GGTGGAAGTG TCTGATTTCC ACCACGCCGC CAGCAAGGCT 
CTGAAAGTGC CCTGCGCTAG TGGAGATTCC GGTGATGGTC TGCTGACCCC CGGGACGCCC 
ATCACACAGT ACATGACCTC TCCTGACCTG CACACTGAGA TCGTGCGGCT CTTCACTGTG 
TTTGCCTTGT GGTTGGATGA TGAAAACCTT CAGAAGCAGG AAATTTACTT GCCCTCTTTG 
CCAAAGCAGT ATGACACCCA TCGCCTGGCC AAAATTATGC AGCGCCAGCA GTATTTTGAG 
GTGTGGATGG AGCATGTGGA TATCGAGCGA GTCCAGCACG AGCTGCAGGA AGTGCTCGAT 
CTGTGGTCAA AGGTCAAGAG TGAACCTGCA GTCTCACAGA ACAGCAGCTC CTCCATCTTT 
ACCGACTTCA TCAACCCTCT GGCTGCAAGG GAGCGTATTC TCACTCACCT GAGAAAGCAT 
GATGCCCCTC AGGGGTCGCT GCTGCCAGTG CCCATGAAAG CTCCAGTGCC TGATATTCCC 
ACTGCGAGCC TAACTGATGA GAAAATCTCC ACCACCTTAA TTCAAGAGGA CCTCACTAAG 
CTCCAGCATC AAGCCAAGTT GGCAACGGTT CGGGAAACCC AGCAGGTTGC TCTAGACAAT 
GAACTGTTGG AGGTGTTGCC ACAGCTCTAC ATAAACCGGG AAGACCAGCT GTCAATGCAG 
TTGGAGTGCC GTGGAAAAAG TGGACAGCCC TGCCAAGGTC CTGCAAATAT CACTGTTAAG 
TACTCAAGTA TGCACAAACT GGAGCCGGTG CAGAATCAGA TCCAGTCTCT GAAGAGTGAC 
ATTAAACAGC TGCAGGCCGA TGGAATCAAA GCTCCTCCTC AGAGTCTGGC TGAGGCGGCC 
GTTCACACTG AGAACTTCAT CACGGCCTTG GTTAATGTTT TCAAGCAAAA TCCATCCCCT 
GGTGTACTGA AGGTGGGCAT CACGGCTTTC TATCAGATAG TGTCGTTTGT GTGTGAGGAC 
ACACAGAGAC ATCCACCAAC CCGCCAGTTC TTCACATCCT GCATTGAGAT CCTCGGCCAG 
GTCTTTATTC GGGACGTCCA ATCAGAATGT AAACACATGC TAAAGACTAT CCTACTCAAC 
AGACGTCTGT GTAACCTACT GTCACCATAC TTCACCCCGA ACGCCTCTCC CTCAGAGCTG 
GTCTCTCTGT ATGAAGAGGT GGTCAACACC CTCCACATCG ACAGCGGTGA TGTCATATTC 
ATGCTCCTCA CAAAGTTTGA CTTGTTTCAG TGGTTGTCGA CGACCCAGCC TCCGTTCTCT 
GAGCGTAGTC GTCTGATGAC CGTCATCCAT CAGGCTCTCT GCACATGTGG GCTGGAGCCT 
GAGCCCGAAG TCCTCATGCC ATTCAACATC TTCTGCAAGC ACTGGACTGA ACTGCTCCTC 
TACCAGTTTC CTGACCACTA CAGCGACTTC CTCCGTCTCC TCATGCAGAG CTCTTCCGAT 
CAGCTTCTTA GTCCTGACTG TTGGAGAACG TCACTGAAAG CTCTGGGCTG TTCTCCTCAA 
ACTAAGAATA AGACAAACAA ATCCCGCTCC ATGCTCCTCT CCCCTCAACA GGTGGATGAA 
ACAATCGAGT GGCTCTCAAA GTACTTCCTA AAGCTCCGAC TGTCTAACGG GGACTTCAGG 
AGCTTTGGTC TTCTTTCAAA ATGGGGGCCA TACATTGAGG AAGTCAAGGT GTTTTGGGAA 
GATCTGATTA CCTACGTCAT TGATCTGGAA CTAAACCATT GCGGCAAAGA ACCTGTAGGA 
AGTCCAAGAC TGCTCAAAGC TCTGGAGAAC CTGCATAGAA AAATGGCCAA ACTCTTTGAG 
CCGTGGATTT TGGTGCTTGA CACTGACAAC ACTAGTCATC CTCGCTGTTC TCCATGGCTT 
GAATCAGACG CCGCAGTTGC AGCGAGCTTA GTAGGCTTAT ATACACGCTT GACAGAGACT 
TTACATGAGA AATTCAAAGA TCGCTTGTTG CCAGGACAGA GAGGAGCTCT GTGGCTTCAT 
CTCATGTTTT ACTGTGAGAC CTGCACTTCT CCTAAAATGC CAGAGTATCT GCTGTACACC 
TACCACACTG AGTACAGCCG TTTGCCCTGG AAGGACCTGC ATCCAGATCA GACACTAATG 
AACCAGTTTT TCAATGTGGA GAGGGGAAGT CCAAAGAGTT GTTTTGTGTT CATGGGAGAG 
TTGCTGTGTG AGGTGAACTG GGTTAGTGTA TTGAGTGACT CTCTCAGCCC CCAGCTGAAC 
CCCTCAACCC ACACAATGGT GGTCTCCGTA CTCCACCTCA TGGTGTTTCT GGCCAAAGAG 
GATCTCAGTA AACCCGAGTC CTCTGTTCTC AGTCTGCTGG ACCAGTCAGC TTCTCTGCCT 
TGGAATCTGG TGGATTTTTC TTCATATCAA AATGTCACGC GCTATGTCAG CACACATTAC 
CCAGCGTCCC TTGTTCTCAG TCACGACACT TCTTCACAGC AGGTCGTCAG ATTGCTCAGA 
ATGGCTGCTG GATTTGAGCC GACCACACAA GTGTTGCATC ACAAGGACAT GACTCTAAAG 
TGTCAAGCCT TCCTTCACCT CATGGTGCAG TTCCTGACCT CGCTGGATCA AAATGGGAAC 
ATCAGTCTGG CATCGCTAGA GACAGAAATG GAGAAATTAC TGGAGAACAT AGTCATTTTC 
AACCCACCTG AGACGGACCT CCAGCAGCGA CACATGGCCA CCTGCAGTCT GTTTGCAGAG 
ATGCTGACTC TGCTGAATGA GGCCGGTATC TCGACAGCAG AGGGTCTGGG CACTAAACTG 
CACTGCTGGG TGGAGAAGCG GGCTCGAGGG CCTCTGCTTC TGCCTTTGCT AACGGCTGCG 
TGCAGATGCC TGGCTTCAGT GCGTCACATG ACACGCACCA CCGAGGCCTG TATTCTCTCC 
TACTTCACCG ATGGAGGATG TGTGGATCAG TACTCTGGAT GGGGGCCCAT CCTGGTGTCT 
CTTCAGGTTC CTGAACTGAC TGTTGAGGAC TTCATTCAGG AAAGCCTAAG TCTGGGCAGC 
TTCCTCACGC TGTACGTCTA CATCCTCCAG AGGCTAAACC TGGAGCAAAC CCTCCTCAAT 
GAGAAGAAAA CCTTAATGCT GCTCAATACG TGGATCAGTC AGGTGTTTCC CAGTGGTCCA 
GCTGATGAGG CCAAGCTGTT TTTATGGTGG CATAAATTCT TGGAGCTTCT GCAAATCCAA 
GTGGACCAAG AGGATGCCTG CTCACTGGAC ACTCTCATTC TCACCTTAAT GGCGTTTCAG 
AATCGTCTCG TTCAACTCGG AGAGGAAAGA CTAAATTCCG GCATCCTGGG AGCCATTGGC 
CTTGGGAGGA AATCACCACT TTCTAACAGG TTTCGGGTTG TGGCGCGCAG CATGTCAGCC 
TTTCTGCTGG TTCAGGTGCC CTCAGAGAAT CAGCTGCGTC TTCATCCCGG GACAGAGCTG 
CAGCTGTCAG CTAAAGCCCA GCAGGCCTTG TCTGCACTGG AGGCGATGGC CAGTAATAAG 
CAGTATTCAG AGCTGCATGT GTCTGTGAAC CAGGCCTGTC AGTTCATCAA GTACCCGAAT 
CACTGTCTGC GAGACGCCAA CACTCTTCTC ACTCTGCTGG TCAATGCACT CTACACTGAC 
CTTCATTACC TGGACATCAT CCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165418.1
CDS	192..7838
Translation	MEAVRPKKAKSKSSGKPQVKKERQSESVRQPSGSSKAVNEPEFTDVPLSLPHVPPDLNTAASQTSLDHHDKAENEQQHVLLCEKPEVVQKSTCHEDKEHELPSQDAAPRLDQTSVLAPSSQSDPKPLSAKLEPFSVPSIYPSIPASLSQPQLSEDSYSISYELLDGSLMPAASERLVAPAVLTLANQESSPPSLVPVEVVDVERSRERLYPELPKTCQSVKPFTSEQLRMWEPGSWLENVELHEAEFQNLAHQEGHELYELLLSYWRCRKQLTQAQTELQAANSDCKNVQNRLWSFKDERLSLQGICADQNKVYGYHCYQQVTLNEAALAELKHLFSAKAEILHQTVALHSYTSILSRLQVESYLYRLLSSNPTTKSVAVQDTSAVSPKSQPSSLQPLKESISVLFIFTRRVLDDSQFQADIHLWLQRLVSVLHQVGSTGDHLFILNHLLCCPAGVGKWAVPFLQIKVLDNPSGVYHFMQALAILMSPARHRPDFLGHMKPSDARGNNSLTEPASGNWTLVDEGGEEDEDPETSWMLLSEDDLVSLFSQFPFDELFKHLLGICSKGDYQPLATTSQKMMKIFAFASSLVELLAVGLQTYNRARYRQFVKRIGHMIRMTLCYVSDHWAQYISLTGVSRTTVQEQSFSMEKLQVEFDHLFLRAVLHVLKAKRLGIWLFMSEMPYGTLSSSMLWKIFYIMQCAETDGLEKLSCSLSAEDCIQRLRESSHQERFQLWLSEINSSDGICLLTTFAHMAQPRRTDVDPEFVRNIVLEIYEVSYVSVTTREIFSKVGRELLAAIAAAHPHIISVLLERLRETIERVGMVSLYLFKELPLHLWMPAQEEVGLIRDWLMNFSLTAVENRLACIILEGLNWGFQPNGCLILPAPLHSDVALLVIEAYQKYLTDKPYGGILSEGIKQVSYLANVVRLGQTPESSFNQWAWDIVLRLKLHANERSPQDAWCPLPSSSAPSVPEITDSPIMHPVFKAVKAGIPIGCFLAIEMTTIGHSLEKFCSEGISLLKTLIQSRHLRAAVHILENILPLVYNCQFYLLKNEQFLSCVQLFLQLDSGTQGVTQQVTQKVAQHLIGPATCENIKLLNSVIQAHVLESSRNGRVGAVAVLEFWLQVLTEQSLWYRDRAVLFLLDHMCRSAFLHQQEECLQKILYQQHKSALGYHGDKGLLSSIMGWIVAGNVTPSFIEGSSLTGEVWFAWMILNMETMFEEDSQLRRCVEHELLSNPACTPDQALKKAQVRLKLQVVPSLQRLMIYRWAQQALATPADHPILPLVWQKFFQLYLRQPGPEFGLEVKGCIGRRYFHSAAHLSLLKSLRQRLVEVSDFHHAASKALKVPCASGDSGDGLLTPGTPITQYMTSPDLHTEIVRLFTVFALWLDDENLQKQEIYLPSLPKQYDTHRLAKIMQRQQYFEVWMEHVDIERVQHELQEVLDLWSKVKSEPAVSQNSSSSIFTDFINPLAARERILTHLRKHDAPQGSLLPVPMKAPVPDIPTASLTDEKISTTLIQEDLTKLQHQAKLATVRETQQVALDNELLEVLPQLYINREDQLSMQLECRGKSGQPCQGPANITVKYSSMHKLEPVQNQIQSLKSDIKQLQADGIKAPPQSLAEAAVHTENFITALVNVFKQNPSPGVLKVGITAFYQIVSFVCEDTQRHPPTRQFFTSCIEILGQVFIRDVQSECKHMLKTILLNRRLCNLLSPYFTPNASPSELVSLYEEVVNTLHIDSGDVIFMLLTKFDLFQWLSTTQPPFSERSRLMTVIHQALCTCGLEPEPEVLMPFNIFCKHWTELLLYQFPDHYSDFLRLLMQSSSDQLLSPDCWRTSLKALGCSPQTKNKTNKSRSMLLSPQQVDETIEWLSKYFLKLRLSNGDFRSFGLLSKWGPYIEEVKVFWEDLITYVIDLELNHCGKEPVGSPRLLKALENLHRKMAKLFEPWILVLDTDNTSHPRCSPWLESDAAVAASLVGLYTRLTETLHEKFKDRLLPGQRGALWLHLMFYCETCTSPKMPEYLLYTYHTEYSRLPWKDLHPDQTLMNQFFNVERGSPKSCFVFMGELLCEVNWVSVLSDSLSPQLNPSTHTMVVSVLHLMVFLAKEDLSKPESSVLSLLDQSASLPWNLVDFSSYQNVTRYVSTHYPASLVLSHDTSSQQVVRLLRMAAGFEPTTQVLHHKDMTLKCQAFLHLMVQFLTSLDQNGNISLASLETEMEKLLENIVIFNPPETDLQQRHMATCSLFAEMLTLLNEAGISTAEGLGTKLHCWVEKRARGPLLLPLLTAACRCLASVRHMTRTTEACILSYFTDGGCVDQYSGWGPILVSLQVPELTVEDFIQESLSLGSFLTLYVYILQRLNLEQTLLNEKKTLMLLNTWISQVFPSGPADEAKLFLWWHKFLELLQIQVDQEDACSLDTLILTLMAFQNRLVQLGEERLNSGILGAIGLGRKSPLSNRFRVVARSMSAFLLVQVPSENQLRLHPGTELQLSAKAQQALSALEAMASNKQYSELHVSVNQACQFIKYPNHCLRDANTLLTLLVNALYTDLHYLDIIR

Target ORF information:

RefSeq Version	XM_005165361.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ectopic P-granules autophagy protein 5 homolog (C. elegans) (epg5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165361.3

ORF Insert Sequence:

ATGGAGGCTG TGAGACCTAA AAAGGCAAAG TCCAAATCCA GTGGGAAGCC ACAGGTCAAA 
AAGGAGAGGC AGTCAGAGAG CGTCAGACAG CCCTCGGGAT CTTCTAAAGC AGTTAATGAG 
CCTGAGTTCA CTGATGTGCC GCTCAGCCTC CCTCATGTGC CTCCTGACCT CAACACAGCT 
GCCTCTCAGA CTTCACTAGA TCATCATGAC AAAGCTGAAA ATGAGCAACA GCATGTTCTC 
TTATGCGAGA AGCCTGAAGT AGTACAGAAA TCGACGTGTC ATGAAGACAA AGAGCATGAA 
TTGCCATCAC AAGATGCTGC ACCTCGGCTG GACCAAACAT CAGTGCTTGC TCCGTCCAGC 
CAAAGTGATC CCAAACCCCT CAGTGCTAAA CTTGAGCCAT TTAGTGTCCC CTCTATATAT 
CCCTCTATCC CAGCTTCCCT CTCCCAGCCT CAGCTTTCAG AGGACTCGTA CTCTATCTCC 
TACGAACTGT TAGATGGCTC TTTAATGCCA GCAGCGTCTG AGAGACTAGT AGCTCCGGCT 
GTTTTGACTC TGGCTAACCA GGAGTCCTCT CCGCCCAGCT TGGTTCCAGT GGAGGTTGTG 
GATGTGGAAA GATCTAGAGA GAGGCTTTAC CCAGAGCTGC CTAAGACCTG CCAGTCTGTG 
AAGCCCTTCA CTAGTGAGCA GCTGCGCATG TGGGAGCCCG GTTCCTGGCT GGAAAACGTG 
GAGCTGCATG AAGCCGAATT CCAGAATCTG GCTCATCAAG AGGGACATGA GCTGTACGAG 
CTGCTGCTGA GCTACTGGAG GTGCCGGAAG CAGCTGACGC AGGCCCAGAC TGAACTGCAG 
GCTGCCAACT CGGACTGCAA AAATGTGCAG AATCGCCTGT GGAGCTTCAA GGATGAAAGG 
CTTTCGTTAC AGGGTATATG TGCAGACCAG AATAAGGTGT ATGGCTACCA CTGCTACCAG 
CAGGTGACAC TGAACGAGGC TGCGTTAGCT GAGCTGAAGC ACCTGTTTTC TGCTAAAGCG 
GAAATCCTCC ATCAGACAGT GGCTCTGCAC TCATACACCT CCATCCTGTC CCGTCTGCAA 
GTGGAGTCTT ATCTCTACCG CTTGCTCAGC AGTAACCCCA CCACCAAGAG TGTGGCTGTG 
CAAGATACAA GTGCAGTGAG TCCAAAGTCT CAGCCCTCGT CTTTGCAGCC GCTGAAGGAA 
AGCATCAGTG TGCTGTTCAT CTTCACCCGA CGGGTCCTAG ATGATTCTCA GTTTCAGGCT 
GACATCCATC TTTGGCTTCA GAGGCTGGTG TCTGTGCTGC ATCAGGTCGG TTCTACAGGA 
GATCATCTAT TCATCCTAAA CCACCTGCTC TGCTGCCCGG CAGGTGTAGG GAAATGGGCT 
GTGCCATTCC TACAGATCAA AGTATTGGAT AACCCATCTG GAGTATATCA TTTCATGCAG 
GCCCTTGCTA TCCTTATGTC TCCAGCAAGG CACCGTCCAG ACTTCCTGGG ACACATGAAG 
CCCAGTGATG CAAGGGGCAA CAACAGCCTA ACAGAACCTG CATCTGGTAA CTGGACTCTA 
GTGGATGAAG GTGGAGAAGA GGACGAAGAC CCAGAAACGA GCTGGATGCT GCTGTCTGAG 
GATGATCTGG TTTCTCTGTT CTCTCAGTTT CCCTTTGATG AGCTCTTCAA ACATTTACTG 
GGCATCTGCT CTAAAGGGGA CTATCAGCCT CTAGCCACCA CAAGTCAAAA GATGATGAAG 
ATATTTGCGT TTGCCTCTTC ACTGGTTGAG TTGCTAGCAG TCGGCTTGCA GACCTACAAC 
AGAGCACGCT ATCGGCAGTT TGTGAAGAGG ATTGGTCACA TGATTCGGAT GACGCTCTGC 
TATGTGAGTG ACCACTGGGC GCAGTACATC AGTCTGACCG GTGTGAGCAG GACCACAGTG 
CAGGAGCAGT CATTTTCAAT GGAGAAACTG CAGGTGGAGT TTGACCATCT CTTCCTGAGG 
GCGGTGCTGC ATGTGCTTAA GGCCAAGAGA CTGGGCATAT GGCTGTTCAT GTCTGAGATG 
CCGTATGGGA CATTGTCCAG CTCCATGCTG TGGAAGATCT TCTACATTAT GCAGTGTGCA 
GAGACAGATG GTCTGGAGAA GCTCAGTTGT AGCTTGAGTG CTGAGGACTG TATCCAGAGG 
CTCAGAGAAT CAAGCCATCA GGAGAGGTTT CAGCTTTGGC TGTCTGAGAT AAACAGCTCA 
GATGGGATCT GCCTGTTGAC AACGTTTGCG CACATGGCCC AGCCGAGACG CACCGATGTA 
GACCCTGAGT TTGTCAGGAA CATAGTGCTA GAGATATACG AGGTTTCCTA TGTAAGTGTC 
ACCACCCGTG AGATCTTCTC TAAAGTTGGA CGGGAATTGC TTGCAGCGAT AGCGGCTGCC 
CACCCTCACA TCATCTCTGT GTTGCTGGAG AGACTGAGAG AGACCATAGA GAGAGTGGGC 
ATGGTGTCGT TGTACCTGTT TAAAGAGTTG CCCTTGCACT TGTGGATGCC AGCTCAGGAG 
GAGGTGGGGC TGATCAGAGA CTGGCTGATG AACTTCAGTC TCACTGCTGT TGAGAACAGA 
TTAGCCTGCA TTATACTAGA GGGCCTCAAC TGGGGATTTC AGCCAAATGG TTGCCTTATT 
TTACCTGCAC CTCTGCACAG TGACGTTGCC TTGTTGGTGA TTGAGGCATA TCAAAAATAT 
CTTACAGATA AACCATATGG TGGCATCCTC TCAGAAGGCA TTAAACAGGT GTCTTACCTG 
GCTAACGTGG TGAGGCTCGG CCAGACTCCA GAATCTTCCT TTAATCAGTG GGCTTGGGAT 
ATAGTTCTGA GGCTAAAGCT CCACGCCAAT GAAAGAAGCC CACAGGATGC ATGGTGCCCG 
CTTCCATCCT CCAGTGCCCC CAGTGTGCCA GAGATCACAG ACTCTCCCAT TATGCACCCA 
GTGTTCAAAG CGGTCAAAGC CGGCATCCCC ATTGGCTGTT TTCTGGCTAT TGAGATGACC 
ACCATCGGGC ACAGTTTGGA GAAGTTTTGC AGTGAAGGAA TATCTTTGCT GAAGACTCTG 
ATTCAGTCCC GTCATCTGAG AGCTGCTGTG CACATACTGG AGAACATTCT TCCTCTGGTC 
TACAACTGCC AGTTCTACCT GCTTAAGAAT GAGCAGTTTC TGAGCTGTGT CCAGCTGTTT 
TTGCAGCTGG ATAGTGGCAC TCAAGGCGTA ACCCAGCAGG TCACACAGAA GGTGGCCCAG 
CATCTGATTG GCCCCGCTAC ATGTGAGAAT ATCAAACTGC TGAACAGTGT GATCCAGGCT 
CATGTTCTGG AGAGCTCCAG GAATGGACGT GTTGGTGCAG TCGCTGTTCT GGAGTTCTGG 
CTTCAGGTTT TGACTGAACA GAGCCTCTGG TATCGAGACA GAGCTGTTCT CTTTCTTCTG 
GACCACATGT GCCGTTCTGC ATTTCTTCAC CAACAAGAGG AATGTCTCCA GAAAATACTC 
TACCAGCAGC ACAAGAGCGC TTTAGGTTAC CATGGAGACA AAGGTTTGCT CTCGTCTATT 
ATGGGCTGGA TAGTGGCAGG AAATGTTACT CCGTCTTTTA TTGAGGGCAG TTCTCTCACA 
GGAGAGGTGT GGTTTGCTTG GATGATCCTA AACATGGAGA CAATGTTCGA GGAAGATTCC 
CAGCTGCGAC GCTGTGTAGA GCATGAGCTG CTCTCAAACC CTGCGTGTAC ACCAGATCAG 
GCTTTGAAGA AAGCCCAGGT GCGTCTCAAG CTTCAGGTTG TCCCATCACT GCAGAGGCTG 
ATGATCTATC GCTGGGCTCA ACAAGCTTTA GCCACTCCTG CAGACCATCC AATCCTGCCT 
TTGGTGTGGC AGAAATTCTT CCAGCTTTAC CTCCGTCAAC CTGGACCAGA GTTTGGGCTG 
GAGGTGAAAG GTTGCATTGG CAGGCGTTAT TTCCATAGTG CGGCCCATCT GTCCTTGCTG 
AAGAGCCTCA GACAGCGGCT GGTGGAAGTG TCTGATTTCC ACCACGCCGC CAGCAAGGCT 
CTGAAAGTGC CCTGCGCTAG TGGAGATTCC GGTGATGGTC TGCTGACCCC CGGGACGCCC 
ATCACACAGT ACATGACCTC TCCTGACCTG CACACTGAGA TCGTGCGGCT CTTCACTGTG 
TTTGCCTTGT GGTTGGATGA TGAAAACCTT CAGAAGCAGG AAATTTACTT GCCCTCTTTG 
CCAAAGCAGT ATGACACCCA TCGCCTGGCC AAAATTATGC AGCGCCAGCA GTATTTTGAG 
GTGTGGATGG AGCATGTGGA TATCGAGCGA GTCCAGCACG AGCTGCAGGA AGTGCTCGAT 
CTGTGGTCAA AGGTCAAGAG TGAACCTGCA GTCTCACAGA ACAGCAGCTC CTCCATCTTT 
ACCGACTTCA TCAACCCTCT GGCTGCAAGG GAGCGTATTC TCACTCACCT GAGAAAGCAT 
GATGCCCCTC AGGGGTCGCT GCTGCCAGTG CCCATGAAAG CTCCAGTGCC TGATATTCCC 
ACTGCGAGCC TAACTGATGA GAAAATCTCC ACCACCTTAA TTCAAGAGGA CCTCACTAAG 
CTCCAGCATC AAGCCAAGTT GGCAACGGTT CGGGAAACCC AGCAGGTTGC TCTAGACAAT 
GAACTGTTGG AGGTGTTGCC ACAGCTCTAC ATAAACCGGG AAGACCAGCT GTCAATGCAG 
TTGGAGTGCC GTGGAAAAAG TGGACAGCCC TGCCAAGGTC CTGCAAATAT CACTGTTAAG 
TACTCAAGTA TGCACAAACT GGAGCCGGTG CAGAATCAGA TCCAGTCTCT GAAGAGTGAC 
ATTAAACAGC TGCAGGCCGA TGGAATCAAA GCTCCTCCTC AGAGTCTGGC TGAGGCGGCC 
GTTCACACTG AGAACTTCAT CACGGCCTTG GTTAATGTTT TCAAGCAAAA TCCATCCCCT 
GGTGTACTGA AGGTGGGCAT CACGGCTTTC TATCAGATAG TGTCGTTTGT GTGTGAGGAC 
ACACAGAGAC ATCCACCAAC CCGCCAGTTC TTCACATCCT GCATTGAGAT CCTCGGCCAG 
GTCTTTATTC GGGACGTCCA ATCAGAATGT AAACACATGC TAAAGACTAT CCTACTCAAC 
AGACGTCTGT GTAACCTACT GTCACCATAC TTCACCCCGA ACGCCTCTCC CTCAGAGCTG 
GTCTCTCTGT ATGAAGAGGT GGTCAACACC CTCCACATCG ACAGCGGTGA TGTCATATTC 
ATGCTCCTCA CAAAGTTTGA CTTGTTTCAG TGGTTGTCGA CGACCCAGCC TCCGTTCTCT 
GAGCGTAGTC GTCTGATGAC CGTCATCCAT CAGGCTCTCT GCACATGTGG GCTGGAGCCT 
GAGCCCGAAG TCCTCATGCC ATTCAACATC TTCTGCAAGC ACTGGACTGA ACTGCTCCTC 
TACCAGTTTC CTGACCACTA CAGCGACTTC CTCCGTCTCC TCATGCAGAG CTCTTCCGAT 
CAGCTTCTTA GTCCTGACTG TTGGAGAACG TCACTGAAAG CTCTGGGCTG TTCTCCTCAA 
ACTAAGAATA AGACAAACAA ATCCCGCTCC ATGCTCCTCT CCCCTCAACA GGTGGATGAA 
ACAATCGAGT GGCTCTCAAA GTACTTCCTA AAGCTCCGAC TGTCTAACGG GGACTTCAGG 
AGCTTTGGTC TTCTTTCAAA ATGGGGGCCA TACATTGAGG AAGTCAAGGT GTTTTGGGAA 
GATCTGATTA CCTACGTCAT TGATCTGGAA CTAAACCATT GCGGCAAAGA ACCTGTAGGA 
AGTCCAAGAC TGCTCAAAGC TCTGGAGAAC CTGCATAGAA AAATGGCCAA ACTCTTTGAG 
CCGTGGATTT TGGTGCTTGA CACTGACAAC ACTAGTCATC CTCGCTGTTC TCCATGGCTT 
GAATCAGACG CCGCAGTTGC AGCGAGCTTA GTAGGCTTAT ATACACGCTT GACAGAGACT 
TTACATGAGA AATTCAAAGA TCGCTTGTTG CCAGGACAGA GAGGAGCTCT GTGGCTTCAT 
CTCATGTTTT ACTGTGAGAC CTGCACTTCT CCTAAAATGC CAGAGTATCT GCTGTACACC 
TACCACACTG AGTACAGCCG TTTGCCCTGG AAGGACCTGC ATCCAGATCA GACACTAATG 
AACCAGTTTT TCAATGTGGA GAGGGGAAGT CCAAAGAGTT GTTTTGTGTT CATGGGAGAG 
TTGCTGTGTG AGGTGAACTG GGTTAGTGTA TTGAGTGACT CTCTCAGCCC CCAGCTGAAC 
CCCTCAACCC ACACAATGGT GGTCTCCGTA CTCCACCTCA TGGTGTTTCT GGCCAAAGAG 
GATCTCAGTA AACCCGAGTC CTCTGTTCTC AGTCTGCTGG ACCAGTCAGC TTCTCTGCCT 
TGGAATCTGG TGGATTTTTC TTCATATCAA AATGTCACGC GCTATGTCAG CACACATTAC 
CCAGCGTCCC TTGTTCTCAG TCACGACACT TCTTCACAGC AGGTCGTCAG ATTGCTCAGA 
ATGGCTGCTG GATTTGAGCC GACCACACAA GTGTTGCATC ACAAGGACAT GACTCTAAAG 
TGTCAAGCCT TCCTTCACCT CATGGTGCAG TTCCTGACCT CGCTGGATCA AAATGGGAAC 
ATCAGTCTGG CATCGCTAGA GACAGAAATG GAGAAATTAC TGGAGAACAT AGTCATTTTC 
AACCCACCTG AGACGGACCT CCAGCAGCGA CACATGGCCA CCTGCAGTCT GTTTGCAGAG 
ATGCTGACTC TGCTGAATGA GGCCGGTATC TCGACAGCAG AGGGTCTGGG CACTAAACTG 
CACTGCTGGG TGGAGAAGCG GGCTCGAGGG CCTCTGCTTC TGCCTTTGCT AACGGCTGCG 
TGCAGATGCC TGGCTTCAGT GCGTCACATG ACACGCACCA CCGAGGCCTG TATTCTCTCC 
TACTTCACCG ATGGAGGATG TGTGGATCAG TACTCTGGAT GGGGGCCCAT CCTGGTGTCT 
CTTCAGGTTC CTGAACTGAC TGTTGAGGAC TTCATTCAGG AAAGCCTAAG TCTGGGCAGC 
TTCCTCACGC TGTACGTCTA CATCCTCCAG AGGCTAAACC TGGAGCAAAC CCTCCTCAAT 
GAGAAGAAAA CCTTAATGCT GCTCAATACG TGGATCAGTC AGGTGTTTCC CAGTGGTCCA 
GCTGATGAGG CCAAGCTGTT TTTATGGTGG CATAAATTCT TGGAGCTTCT GCAAATCCAA 
GTGGACCAAG AGGATGCCTG CTCACTGGAC ACTCTCATTC TCACCTTAAT GGCGTTTCAG 
AATCGTCTCG TTCAACTCGG AGAGGAAAGA CTAAATTCCG GCATCCTGGG AGCCATTGGC 
CTTGGGAGGA AATCACCACT TTCTAACAGG TTTCGGGTTG TGGCGCGCAG CATGTCAGCC 
TTTCTGCTGG TTCAGGTGCC CTCAGAGAAT CAGCTGCGTC TTCATCCCGG GACAGAGCTG 
CAGCTGTCAG CTAAAGCCCA GCAGGCCTTG TCTGCACTGG AGGCGATGGC CAGTAATAAG 
CAGTATTCAG AGCTGCATGT GTCTGTGAAC CAGGCCTGTC AGTTCATCAA GTACCCGAAT 
CACTGTCTGC GAGACGCCAA CACTCTTCTC ACTCTGCTGG TCAATGCACT CTACACTGAC 
CTTCATTACC TGGACATCAT CCGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EPG5 ( XM_009301680.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009299955.1 Danio rerio ectopic P-granules autophagy protein 5 homolog (C. elegans) (epg5), transcript variant X2, mRNA.

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CloneID	ODa45788
Clone ID Related Accession (Same CDS sequence)	XM_009301680.3 , XM_009301680.2
Accession Version	XM_009301680.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ectopic P granules protein 5 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301680.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG TGAGACCTAA AAAGGCAAAG TCCAAATCCA GTGGGAAGCC ACAGGTCAAA 
AAGGAGAGGC AGTCAGAGAG CGTCAGACAG CCCTCGGGAT CTTCTAAAGC AGTTAATGAG 
CCTGAGTTCA CTGATGTGCC GCTCAGCCTC CCTCATGTGC CTCCTGACCT CAACACAGCT 
GCCTCTCAGA CTTCACTAGA TCATCATGAC AAAGCTGAAA ATGAGCAACA GCATGTTCTC 
TTATGCGAGA AGCCTGAAGT AGTACAGAAA TCGACGTGTC ATGAAGACAA AGAGCATGAA 
TTGCCATCAC AAGATGCTGC ACCTCGGCTG GACCAAACAT CAGTGCTTGC TCCGTCCAGC 
CAAAGTGATC CCAAACCCCT CAGTGCTAAA CTTGAGCCAT TTAGTGTCCC CTCTATATAT 
CCCTCTATCC CAGCTTCCCT CTCCCAGCCT CAGCTTTCAG AGGACTCGTA CTCTATCTCC 
TACGAACTGT TAGATGGCTC TTTAATGCCA GCAGCGTCTG AGAGACTAGT AGCTCCGGCT 
GTTTTGACTC TGGCTAACCA GGAGTCCTCT CCGCCCAGCT TGGTTCCAGT GGAGGTTGTG 
GATGTGGAAA GATCTAGAGA GAGGCTTTAC CCAGAGCTGC CTAAGACCTG CCAGTCTGTG 
AAGCCCTTCA CTAGTGAGCA GCTGCGCATG TGGGAGCCCG GTTCCTGGCT GGAAAACGTG 
GAGCTGCATG AAGCCGAATT CCAGAATCTG GCTCATCAAG AGGGACATGA GCTGTACGAG 
CTGCTGCTGA GCTACTGGAG GTGCCGGAAG CAGCTGACGC AGGCCCAGAC TGAACTGCAG 
GCTGCCAACT CGGACTGCAA AAATGTGCAG AATCGCCTGT GGAGCTTCAA GGATGAAAGG 
CTTTCGTTAC AGGGTATATG TGCAGACCAG AATAAGGTGT ATGGCTACCA CTGCTACCAG 
CAGGTGACAC TGAACGAGGC TGCGTTAGCT GAGCTGAAGC ACCTGTTTTC TGCTAAAGCG 
GAAATCCTCC ATCAGACAGT GGCTCTGCAC TCATACACCT CCATCCTGTC CCGTCTGCAA 
GTGGAGTCTT ATCTCTACCG CTTGCTCAGC AGTAACCCCA CCACCAAGAG TGTGGCTGTG 
CAAGATACAA GTGCAGTGAG TCCAAAGTCT CAGCCCTCGT CTTTGCAGCC GCTGAAGGAA 
AGCATCAGTG TGCTGTTCAT CTTCACCCGA CGGGTCCTAG ATGATTCTCA GTTTCAGGCT 
GACATCCATC TTTGGCTTCA GAGGCTGGTG TCTGTGCTGC ATCAGGTCGG TTCTACAGGA 
GATCATCTAT TCATCCTAAA CCACCTGCTC TGCTGCCCGG CAGGTGTAGG GAAATGGGCT 
GTGCCATTCC TACAGATCAA AGTATTGGAT AACCCATCTG GAGTATATCA TTTCATGCAG 
GCCCTTGCTA TCCTTATGTC TCCAGCAAGG CACCGTCCAG ACTTCCTGGG ACACATGAAG 
CCCAGTGATG CAAGGGGCAA CAACAGCCTA ACAGAACCTG CATCTGGTAA CTGGACTCTA 
GTGGATGAAG GTGGAGAAGA GGACGAAGAC CCAGAAACGA GCTGGATGCT GCTGTCTGAG 
GATGATCTGG TTTCTCTGTT CTCTCAGTTT CCCTTTGATG AGCTCTTCAA ACATTTACTG 
GGCATCTGCT CTAAAGGGGA CTATCAGCCT CTAGCCACCA CAAGTCAAAA GATGATGAAG 
ATATTTGCGT TTGCCTCTTC ACTGGTTGAG TTGCTAGCAG TCGGCTTGCA GACCTACAAC 
AGAGCACGCT ATCGGCAGTT TGTGAAGAGG ATTGGTCACA TGATTCGGAT GACGCTCTGC 
TATGTGAGTG ACCACTGGGC GCAGTACATC AGTCTGACCG GTGTGAGCAG GACCACAGTG 
CAGGAGCAGT CATTTTCAAT GGAGAAACTG CAGGTGGAGT TTGACCATCT CTTCCTGAGG 
GCGGTGCTGC ATGTGCTTAA GGCCAAGAGA CTGGGCATAT GGCTGTTCAT GTCTGAGATG 
CCGTATGGGA CATTGTCCAG CTCCATGCTG TGGAAGATCT TCTACATTAT GCAGTGTGCA 
GAGACAGATG GTCTGGAGAA GCTCAGTTGT AGCTTGAGTG CTGAGGACTG TATCCAGAGG 
CTCAGAGAAT CAAGCCATCA GGAGAGGTTT CAGCTTTGGC TGTCTGAGAT AAACAGCTCA 
GATGGGATCT GCCTGTTGAC AACGTTTGCG CACATGGCCC AGCCGAGACG CACCGATGTA 
GACCCTGAGT TTGTCAGGAA CATAGTGCTA GAGATATACG AGGTTTCCTA TGTAAGTGTC 
ACCACCCGTG AGATCTTCTC TAAAGTTGGA CGGGAATTGC TTGCAGCGAT AGCGGCTGCC 
CACCCTCACA TCATCTCTGT GTTGCTGGAG AGACTGAGAG AGACCATAGA GAGAGTGGGC 
ATGGTGTCGT TGTACCTGTT TAAAGAGTTG CCCTTGCACT TGTGGATGCC AGCTCAGGAG 
GAGGTGGGGC TGATCAGAGA CTGGCTGATG AACTTCAGTC TCACTGCTGT TGAGAACAGA 
TTAGCCTGCA TTATACTAGA GGGCCTCAAC TGGGGATTTC AGCCAAATGG TTGCCTTATT 
TTACCTGCAC CTCTGCACAG TGACGTTGCC TTGTTGGTGA TTGAGGCATA TCAAAAATAT 
CTTACAGATA AACCATATGG TGGCATCCTC TCAGAAGGCA TTAAACAGGT GTCTTACCTG 
GCTAACGTGG TGAGGCTCGG CCAGACTCCA GAATCTTCCT TTAATCAGTG GGCTTGGGAT 
ATAGTTCTGA GGCTAAAGCT CCACGCCAAT GAAAGAAGCC CACAGGATGC ATGGTGCCCG 
CTTCCATCCT CCAGTGCCCC CAGTGTGCCA GAGATCACAG ACTCTCCCAT TATGCACCCA 
GTGTTCAAAG CGGTCAAAGC CGGCATCCCC ATTGGCTGTT TTCTGGCTAT TGAGATGACC 
ACCATCGGGC ACAGTTTGGA GAAGTTTTGC AGTGAAGGAA TATCTTTGCT GAAGACTCTG 
ATTCAGTCCC GTCATCTGAG AGCTGCTGTG CACATACTGG AGAACATTCT TCCTCTGGTC 
TACAACTGCC AGTTCTACCT GCTTAAGAAT GAGCAGTTTC TGAGCTGTGT CCAGCTGTTT 
TTGCAGCTGG ATAGTGGCAC TCAAGGCGTA ACCCAGCAGG TCACACAGAA GGTGGCCCAG 
CATCTGATTG GCCCCGCTAC ATGTGAGAAT ATCAAACTGC TGAACAGTGT GATCCAGGCT 
CATGTTCTGG AGAGCTCCAG GAATGGACGT GTTGGTGCAG TCGCTGTTCT GGAGTTCTGG 
CTTCAGGTTT TGACTGAACA GAGCCTCTGG TATCGAGACA GAGCTGTTCT CTTTCTTCTG 
GACCACATGT GCCGTTCTGC ATTTCTTCAC CAACAAGAGG AATGTCTCCA GAAAATACTC 
TACCAGCAGC ACAAGAGCGC TTTAGGTTAC CATGGAGACA AAGGTTTGCT CTCGTCTATT 
ATGGGCTGGA TAGTGGCAGG AAATGTTACT CCGTCTTTTA TTGAGGGCAG TTCTCTCACA 
GGAGAGGTGT GGTTTGCTTG GATGATCCTA AACATGGAGA CAATGTTCGA GGAAGATTCC 
CAGCTGCGAC GCTGTGTAGA GCATGAGCTG CTCTCAAACC CTGCGTGTAC ACCAGATCAG 
GCTTTGAAGA AAGCCCAGGT GCGTCTCAAG CTTCAGGTTG TCCCATCACT GCAGAGGCTG 
ATGATCTATC GCTGGGCTCA ACAAGCTTTA GCCACTCCTG CAGACCATCC AATCCTGCCT 
TTGGTGTGGC AGAAATTCTT CCAGCTTTAC CTCCGTCAAC CTGGACCAGA GTTTGGGCTG 
GAGGTGAAAG GTTGCATTGG CAGGCGTTAT TTCCATAGTG CGGCCCATCT GTCCTTGCTG 
AAGAGCCTCA GACAGCGGCT GGTGGAAGTG TCTGATTTCC ACCACGCCGC CAGCAAGGCT 
CTGAAAGTGC CCTGCGCTAG TGGAGATTCC GGTGATGGTC TGCTGACCCC CGGGACGCCC 
ATCACACAGT ACATGACCTC TCCTGACCTG CACACTGAGA TCGTGCGGCT CTTCACTGTG 
TTTGCCTTGT GGTTGGATGA TGAAAACCTT CAGAAGCAGG AAATTTACTT GCCCTCTTTG 
CCAAAGCAGT ATGACACCCA TCGCCTGGCC AAAATTATGC AGCGCCAGCA GGAGGTGTGG 
ATGGAGCATG TGGATATCGA GCGAGTCCAG CACGAGCTGC AGGAAGTGCT CGATCTGTGG 
TCAAAGGTCA AGAGTGAACC TGCAGTCTCA CAGAACAGCA GCTCCTCCAT CTTTACCGAC 
TTCATCAACC CTCTGGCTGC AAGGGAGCGT ATTCTCACTC ACCTGAGAAA GCATGATGCC 
CCTCAGGGGT CGCTGCTGCC AGTGCCCATG AAAGCTCCAG TGCCTGATAT TCCCACTGCG 
AGCCTAACTG ATGAGAAAAT CTCCACCACC TTAATTCAAG AGGACCTCAC TAAGCTCCAG 
CATCAAGCCA AGTTGGCAAC GGTTCGGGAA ACCCAGCAGG TTGCTCTAGA CAATGAACTG 
TTGGAGGTGT TGCCACAGCT CTACATAAAC CGGGAAGACC AGCTGTCAAT GCAGTTGGAG 
TGCCGTGGAA AAAGTGGACA GCCCTGCCAA GGTCCTGCAA ATATCACTGT TAAGTACTCA 
AGTATGCACA AACTGGAGCC GGTGCAGAAT CAGATCCAGT CTCTGAAGAG TGACATTAAA 
CAGCTGCAGG CCGATGGAAT CAAAGCTCCT CCTCAGAGTC TGGCTGAGGC GGCCGTTCAC 
ACTGAGAACT TCATCACGGC CTTGGTTAAT GTTTTCAAGC AAAATCCATC CCCTGGTGTA 
CTGAAGGTGG GCATCACGGC TTTCTATCAG ATAGTGTCGT TTGTGTGTGA GGACACACAG 
AGACATCCAC CAACCCGCCA GTTCTTCACA TCCTGCATTG AGATCCTCGG CCAGGTCTTT 
ATTCGGGACG TCCAATCAGA ATGTAAACAC ATGCTAAAGA CTATCCTACT CAACAGACGT 
CTGTGTAACC TACTGTCACC ATACTTCACC CCGAACGCCT CTCCCTCAGA GCTGGTCTCT 
CTGTATGAAG AGGTGGTCAA CACCCTCCAC ATCGACAGCG GTGATGTCAT ATTCATGCTC 
CTCACAAAGT TTGACTTGTT TCAGTGGTTG TCGACGACCC AGCCTCCGTT CTCTGAGCGT 
AGTCGTCTGA TGACCGTCAT CCATCAGGCT CTCTGCACAT GTGGGCTGGA GCCTGAGCCC 
GAAGTCCTCA TGCCATTCAA CATCTTCTGC AAGCACTGGA CTGAACTGCT CCTCTACCAG 
TTTCCTGACC ACTACAGCGA CTTCCTCCGT CTCCTCATGC AGAGCTCTTC CGATCAGCTT 
CTTAGTCCTG ACTGTTGGAG AACGTCACTG AAAGCTCTGG GCTGTTCTCC TCAAACTAAG 
AATAAGACAA ACAAATCCCG CTCCATGCTC CTCTCCCCTC AACAGGTGGA TGAAACAATC 
GAGTGGCTCT CAAAGTACTT CCTAAAGCTC CGACTGTCTA ACGGGGACTT CAGGAGCTTT 
GGTCTTCTTT CAAAATGGGG GCCATACATT GAGGAAGTCA AGGTGTTTTG GGAAGATCTG 
ATTACCTACG TCATTGATCT GGAACTAAAC CATTGCGGCA AAGAACCTGT AGGAAGTCCA 
AGACTGCTCA AAGCTCTGGA GAACCTGCAT AGAAAAATGG CCAAACTCTT TGAGCCGTGG 
ATTTTGGTGC TTGACACTGA CAACACTAGT CATCCTCGCT GTTCTCCATG GCTTGAATCA 
GACGCCGCAG TTGCAGCGAG CTTAGTAGGC TTATATACAC GCTTGACAGA GACTTTACAT 
GAGAAATTCA AAGATCGCTT GTTGCCAGGA CAGAGAGGAG CTCTGTGGCT TCATCTCATG 
TTTTACTGTG AGACCTGCAC TTCTCCTAAA ATGCCAGAGT ATCTGCTGTA CACCTACCAC 
ACTGAGTACA GCCGTTTGCC CTGGAAGGAC CTGCATCCAG ATCAGACACT AATGAACCAG 
TTTTTCAATG TGGAGAGGGG AAGTCCAAAG AGTTGTTTTG TGTTCATGGG AGAGTTGCTG 
TGTGAGGTGA ACTGGGTTAG TGTATTGAGT GACTCTCTCA GCCCCCAGCT GAACCCCTCA 
ACCCACACAA TGGTGGTCTC CGTACTCCAC CTCATGGTGT TTCTGGCCAA AGAGGATCTC 
AGTAAACCCG AGTCCTCTGT TCTCAGTCTG CTGGACCAGT CAGCTTCTCT GCCTTGGAAT 
CTGGTGGATT TTTCTTCATA TCAAAATGTC ACGCGCTATG TCAGCACACA TTACCCAGCG 
TCCCTTGTTC TCAGTCACGA CACTTCTTCA CAGCAGGTCG TCAGATTGCT CAGAATGGCT 
GCTGGATTTG AGCCGACCAC ACAAGTGTTG CATCACAAGG ACATGACTCT AAAGTGTCAA 
GCCTTCCTTC ACCTCATGGT GCAGTTCCTG ACCTCGCTGG ATCAAAATGG GAACATCAGT 
CTGGCATCGC TAGAGACAGA AATGGAGAAA TTACTGGAGA ACATAGTCAT TTTCAACCCA 
CCTGAGACGG ACCTCCAGCA GCGACACATG GCCACCTGCA GTCTGTTTGC AGAGATGCTG 
ACTCTGCTGA ATGAGGCCGG TATCTCGACA GCAGAGGGTC TGGGCACTAA ACTGCACTGC 
TGGGTGGAGA AGCGGGCTCG AGGGCCTCTG CTTCTGCCTT TGCTAACGGC TGCGTGCAGA 
TGCCTGGCTT CAGTGCGTCA CATGACACGC ACCACCGAGG CCTGTATTCT CTCCTACTTC 
ACCGATGGAG GATGTGTGGA TCAGTACTCT GGATGGGGGC CCATCCTGGT GTCTCTTCAG 
GTTCCTGAAC TGACTGTTGA GGACTTCATT CAGGAAAGCC TAAGTCTGGG CAGCTTCCTC 
ACGCTGTACG TCTACATCCT CCAGAGGCTA AACCTGGAGC AAACCCTCCT CAATGAGAAG 
AAAACCTTAA TGCTGCTCAA TACGTGGATC AGTCAGGTGT TTCCCAGTGG TCCAGCTGAT 
GAGGCCAAGC TGTTTTTATG GTGGCATAAA TTCTTGGAGC TTCTGCAAAT CCAAGTGGAC 
CAAGAGGATG CCTGCTCACT GGACACTCTC ATTCTCACCT TAATGGCGTT TCAGAATCGT 
CTCGTTCAAC TCGGAGAGGA AAGACTAAAT TCCGGCATCC TGGGAGCCAT TGGCCTTGGG 
AGGAAATCAC CACTTTCTAA CAGGTTTCGG GTTGTGGCGC GCAGCATGTC AGCCTTTCTG 
CTGGTTCAGG TGCCCTCAGA GAATCAGCTG CGTCTTCATC CCGGGACAGA GCTGCAGCTG 
TCAGCTAAAG CCCAGCAGGC CTTGTCTGCA CTGGAGGCGA TGGCCAGTAA TAAGCAGTAT 
TCAGAGCTGC ATGTGTCTGT GAACCAGGCC TGTCAGTTCA TCAAGTACCC GAATCACTGT 
CTGCGAGACG CCAACACTCT TCTCACTCTG CTGGTCAATG CACTCTACAC TGACCTTCAT 
TACCTGGACA TCATCCGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299955.1
CDS	193..7833
Translation	MEAVRPKKAKSKSSGKPQVKKERQSESVRQPSGSSKAVNEPEFTDVPLSLPHVPPDLNTAASQTSLDHHDKAENEQQHVLLCEKPEVVQKSTCHEDKEHELPSQDAAPRLDQTSVLAPSSQSDPKPLSAKLEPFSVPSIYPSIPASLSQPQLSEDSYSISYELLDGSLMPAASERLVAPAVLTLANQESSPPSLVPVEVVDVERSRERLYPELPKTCQSVKPFTSEQLRMWEPGSWLENVELHEAEFQNLAHQEGHELYELLLSYWRCRKQLTQAQTELQAANSDCKNVQNRLWSFKDERLSLQGICADQNKVYGYHCYQQVTLNEAALAELKHLFSAKAEILHQTVALHSYTSILSRLQVESYLYRLLSSNPTTKSVAVQDTSAVSPKSQPSSLQPLKESISVLFIFTRRVLDDSQFQADIHLWLQRLVSVLHQVGSTGDHLFILNHLLCCPAGVGKWAVPFLQIKVLDNPSGVYHFMQALAILMSPARHRPDFLGHMKPSDARGNNSLTEPASGNWTLVDEGGEEDEDPETSWMLLSEDDLVSLFSQFPFDELFKHLLGICSKGDYQPLATTSQKMMKIFAFASSLVELLAVGLQTYNRARYRQFVKRIGHMIRMTLCYVSDHWAQYISLTGVSRTTVQEQSFSMEKLQVEFDHLFLRAVLHVLKAKRLGIWLFMSEMPYGTLSSSMLWKIFYIMQCAETDGLEKLSCSLSAEDCIQRLRESSHQERFQLWLSEINSSDGICLLTTFAHMAQPRRTDVDPEFVRNIVLEIYEVSYVSVTTREIFSKVGRELLAAIAAAHPHIISVLLERLRETIERVGMVSLYLFKELPLHLWMPAQEEVGLIRDWLMNFSLTAVENRLACIILEGLNWGFQPNGCLILPAPLHSDVALLVIEAYQKYLTDKPYGGILSEGIKQVSYLANVVRLGQTPESSFNQWAWDIVLRLKLHANERSPQDAWCPLPSSSAPSVPEITDSPIMHPVFKAVKAGIPIGCFLAIEMTTIGHSLEKFCSEGISLLKTLIQSRHLRAAVHILENILPLVYNCQFYLLKNEQFLSCVQLFLQLDSGTQGVTQQVTQKVAQHLIGPATCENIKLLNSVIQAHVLESSRNGRVGAVAVLEFWLQVLTEQSLWYRDRAVLFLLDHMCRSAFLHQQEECLQKILYQQHKSALGYHGDKGLLSSIMGWIVAGNVTPSFIEGSSLTGEVWFAWMILNMETMFEEDSQLRRCVEHELLSNPACTPDQALKKAQVRLKLQVVPSLQRLMIYRWAQQALATPADHPILPLVWQKFFQLYLRQPGPEFGLEVKGCIGRRYFHSAAHLSLLKSLRQRLVEVSDFHHAASKALKVPCASGDSGDGLLTPGTPITQYMTSPDLHTEIVRLFTVFALWLDDENLQKQEIYLPSLPKQYDTHRLAKIMQRQQEVWMEHVDIERVQHELQEVLDLWSKVKSEPAVSQNSSSSIFTDFINPLAARERILTHLRKHDAPQGSLLPVPMKAPVPDIPTASLTDEKISTTLIQEDLTKLQHQAKLATVRETQQVALDNELLEVLPQLYINREDQLSMQLECRGKSGQPCQGPANITVKYSSMHKLEPVQNQIQSLKSDIKQLQADGIKAPPQSLAEAAVHTENFITALVNVFKQNPSPGVLKVGITAFYQIVSFVCEDTQRHPPTRQFFTSCIEILGQVFIRDVQSECKHMLKTILLNRRLCNLLSPYFTPNASPSELVSLYEEVVNTLHIDSGDVIFMLLTKFDLFQWLSTTQPPFSERSRLMTVIHQALCTCGLEPEPEVLMPFNIFCKHWTELLLYQFPDHYSDFLRLLMQSSSDQLLSPDCWRTSLKALGCSPQTKNKTNKSRSMLLSPQQVDETIEWLSKYFLKLRLSNGDFRSFGLLSKWGPYIEEVKVFWEDLITYVIDLELNHCGKEPVGSPRLLKALENLHRKMAKLFEPWILVLDTDNTSHPRCSPWLESDAAVAASLVGLYTRLTETLHEKFKDRLLPGQRGALWLHLMFYCETCTSPKMPEYLLYTYHTEYSRLPWKDLHPDQTLMNQFFNVERGSPKSCFVFMGELLCEVNWVSVLSDSLSPQLNPSTHTMVVSVLHLMVFLAKEDLSKPESSVLSLLDQSASLPWNLVDFSSYQNVTRYVSTHYPASLVLSHDTSSQQVVRLLRMAAGFEPTTQVLHHKDMTLKCQAFLHLMVQFLTSLDQNGNISLASLETEMEKLLENIVIFNPPETDLQQRHMATCSLFAEMLTLLNEAGISTAEGLGTKLHCWVEKRARGPLLLPLLTAACRCLASVRHMTRTTEACILSYFTDGGCVDQYSGWGPILVSLQVPELTVEDFIQESLSLGSFLTLYVYILQRLNLEQTLLNEKKTLMLLNTWISQVFPSGPADEAKLFLWWHKFLELLQIQVDQEDACSLDTLILTLMAFQNRLVQLGEERLNSGILGAIGLGRKSPLSNRFRVVARSMSAFLLVQVPSENQLRLHPGTELQLSAKAQQALSALEAMASNKQYSELHVSVNQACQFIKYPNHCLRDANTLLTLLVNALYTDLHYLDIIR

Target ORF information:

RefSeq Version	XM_009301680.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ectopic P-granules autophagy protein 5 homolog (C. elegans) (epg5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301680.3

ORF Insert Sequence:

ATGGAGGCTG TGAGACCTAA AAAGGCAAAG TCCAAATCCA GTGGGAAGCC ACAGGTCAAA 
AAGGAGAGGC AGTCAGAGAG CGTCAGACAG CCCTCGGGAT CTTCTAAAGC AGTTAATGAG 
CCTGAGTTCA CTGATGTGCC GCTCAGCCTC CCTCATGTGC CTCCTGACCT CAACACAGCT 
GCCTCTCAGA CTTCACTAGA TCATCATGAC AAAGCTGAAA ATGAGCAACA GCATGTTCTC 
TTATGCGAGA AGCCTGAAGT AGTACAGAAA TCGACGTGTC ATGAAGACAA AGAGCATGAA 
TTGCCATCAC AAGATGCTGC ACCTCGGCTG GACCAAACAT CAGTGCTTGC TCCGTCCAGC 
CAAAGTGATC CCAAACCCCT CAGTGCTAAA CTTGAGCCAT TTAGTGTCCC CTCTATATAT 
CCCTCTATCC CAGCTTCCCT CTCCCAGCCT CAGCTTTCAG AGGACTCGTA CTCTATCTCC 
TACGAACTGT TAGATGGCTC TTTAATGCCA GCAGCGTCTG AGAGACTAGT AGCTCCGGCT 
GTTTTGACTC TGGCTAACCA GGAGTCCTCT CCGCCCAGCT TGGTTCCAGT GGAGGTTGTG 
GATGTGGAAA GATCTAGAGA GAGGCTTTAC CCAGAGCTGC CTAAGACCTG CCAGTCTGTG 
AAGCCCTTCA CTAGTGAGCA GCTGCGCATG TGGGAGCCCG GTTCCTGGCT GGAAAACGTG 
GAGCTGCATG AAGCCGAATT CCAGAATCTG GCTCATCAAG AGGGACATGA GCTGTACGAG 
CTGCTGCTGA GCTACTGGAG GTGCCGGAAG CAGCTGACGC AGGCCCAGAC TGAACTGCAG 
GCTGCCAACT CGGACTGCAA AAATGTGCAG AATCGCCTGT GGAGCTTCAA GGATGAAAGG 
CTTTCGTTAC AGGGTATATG TGCAGACCAG AATAAGGTGT ATGGCTACCA CTGCTACCAG 
CAGGTGACAC TGAACGAGGC TGCGTTAGCT GAGCTGAAGC ACCTGTTTTC TGCTAAAGCG 
GAAATCCTCC ATCAGACAGT GGCTCTGCAC TCATACACCT CCATCCTGTC CCGTCTGCAA 
GTGGAGTCTT ATCTCTACCG CTTGCTCAGC AGTAACCCCA CCACCAAGAG TGTGGCTGTG 
CAAGATACAA GTGCAGTGAG TCCAAAGTCT CAGCCCTCGT CTTTGCAGCC GCTGAAGGAA 
AGCATCAGTG TGCTGTTCAT CTTCACCCGA CGGGTCCTAG ATGATTCTCA GTTTCAGGCT 
GACATCCATC TTTGGCTTCA GAGGCTGGTG TCTGTGCTGC ATCAGGTCGG TTCTACAGGA 
GATCATCTAT TCATCCTAAA CCACCTGCTC TGCTGCCCGG CAGGTGTAGG GAAATGGGCT 
GTGCCATTCC TACAGATCAA AGTATTGGAT AACCCATCTG GAGTATATCA TTTCATGCAG 
GCCCTTGCTA TCCTTATGTC TCCAGCAAGG CACCGTCCAG ACTTCCTGGG ACACATGAAG 
CCCAGTGATG CAAGGGGCAA CAACAGCCTA ACAGAACCTG CATCTGGTAA CTGGACTCTA 
GTGGATGAAG GTGGAGAAGA GGACGAAGAC CCAGAAACGA GCTGGATGCT GCTGTCTGAG 
GATGATCTGG TTTCTCTGTT CTCTCAGTTT CCCTTTGATG AGCTCTTCAA ACATTTACTG 
GGCATCTGCT CTAAAGGGGA CTATCAGCCT CTAGCCACCA CAAGTCAAAA GATGATGAAG 
ATATTTGCGT TTGCCTCTTC ACTGGTTGAG TTGCTAGCAG TCGGCTTGCA GACCTACAAC 
AGAGCACGCT ATCGGCAGTT TGTGAAGAGG ATTGGTCACA TGATTCGGAT GACGCTCTGC 
TATGTGAGTG ACCACTGGGC GCAGTACATC AGTCTGACCG GTGTGAGCAG GACCACAGTG 
CAGGAGCAGT CATTTTCAAT GGAGAAACTG CAGGTGGAGT TTGACCATCT CTTCCTGAGG 
GCGGTGCTGC ATGTGCTTAA GGCCAAGAGA CTGGGCATAT GGCTGTTCAT GTCTGAGATG 
CCGTATGGGA CATTGTCCAG CTCCATGCTG TGGAAGATCT TCTACATTAT GCAGTGTGCA 
GAGACAGATG GTCTGGAGAA GCTCAGTTGT AGCTTGAGTG CTGAGGACTG TATCCAGAGG 
CTCAGAGAAT CAAGCCATCA GGAGAGGTTT CAGCTTTGGC TGTCTGAGAT AAACAGCTCA 
GATGGGATCT GCCTGTTGAC AACGTTTGCG CACATGGCCC AGCCGAGACG CACCGATGTA 
GACCCTGAGT TTGTCAGGAA CATAGTGCTA GAGATATACG AGGTTTCCTA TGTAAGTGTC 
ACCACCCGTG AGATCTTCTC TAAAGTTGGA CGGGAATTGC TTGCAGCGAT AGCGGCTGCC 
CACCCTCACA TCATCTCTGT GTTGCTGGAG AGACTGAGAG AGACCATAGA GAGAGTGGGC 
ATGGTGTCGT TGTACCTGTT TAAAGAGTTG CCCTTGCACT TGTGGATGCC AGCTCAGGAG 
GAGGTGGGGC TGATCAGAGA CTGGCTGATG AACTTCAGTC TCACTGCTGT TGAGAACAGA 
TTAGCCTGCA TTATACTAGA GGGCCTCAAC TGGGGATTTC AGCCAAATGG TTGCCTTATT 
TTACCTGCAC CTCTGCACAG TGACGTTGCC TTGTTGGTGA TTGAGGCATA TCAAAAATAT 
CTTACAGATA AACCATATGG TGGCATCCTC TCAGAAGGCA TTAAACAGGT GTCTTACCTG 
GCTAACGTGG TGAGGCTCGG CCAGACTCCA GAATCTTCCT TTAATCAGTG GGCTTGGGAT 
ATAGTTCTGA GGCTAAAGCT CCACGCCAAT GAAAGAAGCC CACAGGATGC ATGGTGCCCG 
CTTCCATCCT CCAGTGCCCC CAGTGTGCCA GAGATCACAG ACTCTCCCAT TATGCACCCA 
GTGTTCAAAG CGGTCAAAGC CGGCATCCCC ATTGGCTGTT TTCTGGCTAT TGAGATGACC 
ACCATCGGGC ACAGTTTGGA GAAGTTTTGC AGTGAAGGAA TATCTTTGCT GAAGACTCTG 
ATTCAGTCCC GTCATCTGAG AGCTGCTGTG CACATACTGG AGAACATTCT TCCTCTGGTC 
TACAACTGCC AGTTCTACCT GCTTAAGAAT GAGCAGTTTC TGAGCTGTGT CCAGCTGTTT 
TTGCAGCTGG ATAGTGGCAC TCAAGGCGTA ACCCAGCAGG TCACACAGAA GGTGGCCCAG 
CATCTGATTG GCCCCGCTAC ATGTGAGAAT ATCAAACTGC TGAACAGTGT GATCCAGGCT 
CATGTTCTGG AGAGCTCCAG GAATGGACGT GTTGGTGCAG TCGCTGTTCT GGAGTTCTGG 
CTTCAGGTTT TGACTGAACA GAGCCTCTGG TATCGAGACA GAGCTGTTCT CTTTCTTCTG 
GACCACATGT GCCGTTCTGC ATTTCTTCAC CAACAAGAGG AATGTCTCCA GAAAATACTC 
TACCAGCAGC ACAAGAGCGC TTTAGGTTAC CATGGAGACA AAGGTTTGCT CTCGTCTATT 
ATGGGCTGGA TAGTGGCAGG AAATGTTACT CCGTCTTTTA TTGAGGGCAG TTCTCTCACA 
GGAGAGGTGT GGTTTGCTTG GATGATCCTA AACATGGAGA CAATGTTCGA GGAAGATTCC 
CAGCTGCGAC GCTGTGTAGA GCATGAGCTG CTCTCAAACC CTGCGTGTAC ACCAGATCAG 
GCTTTGAAGA AAGCCCAGGT GCGTCTCAAG CTTCAGGTTG TCCCATCACT GCAGAGGCTG 
ATGATCTATC GCTGGGCTCA ACAAGCTTTA GCCACTCCTG CAGACCATCC AATCCTGCCT 
TTGGTGTGGC AGAAATTCTT CCAGCTTTAC CTCCGTCAAC CTGGACCAGA GTTTGGGCTG 
GAGGTGAAAG GTTGCATTGG CAGGCGTTAT TTCCATAGTG CGGCCCATCT GTCCTTGCTG 
AAGAGCCTCA GACAGCGGCT GGTGGAAGTG TCTGATTTCC ACCACGCCGC CAGCAAGGCT 
CTGAAAGTGC CCTGCGCTAG TGGAGATTCC GGTGATGGTC TGCTGACCCC CGGGACGCCC 
ATCACACAGT ACATGACCTC TCCTGACCTG CACACTGAGA TCGTGCGGCT CTTCACTGTG 
TTTGCCTTGT GGTTGGATGA TGAAAACCTT CAGAAGCAGG AAATTTACTT GCCCTCTTTG 
CCAAAGCAGT ATGACACCCA TCGCCTGGCC AAAATTATGC AGCGCCAGCA GGAGGTGTGG 
ATGGAGCATG TGGATATCGA GCGAGTCCAG CACGAGCTGC AGGAAGTGCT CGATCTGTGG 
TCAAAGGTCA AGAGTGAACC TGCAGTCTCA CAGAACAGCA GCTCCTCCAT CTTTACCGAC 
TTCATCAACC CTCTGGCTGC AAGGGAGCGT ATTCTCACTC ACCTGAGAAA GCATGATGCC 
CCTCAGGGGT CGCTGCTGCC AGTGCCCATG AAAGCTCCAG TGCCTGATAT TCCCACTGCG 
AGCCTAACTG ATGAGAAAAT CTCCACCACC TTAATTCAAG AGGACCTCAC TAAGCTCCAG 
CATCAAGCCA AGTTGGCAAC GGTTCGGGAA ACCCAGCAGG TTGCTCTAGA CAATGAACTG 
TTGGAGGTGT TGCCACAGCT CTACATAAAC CGGGAAGACC AGCTGTCAAT GCAGTTGGAG 
TGCCGTGGAA AAAGTGGACA GCCCTGCCAA GGTCCTGCAA ATATCACTGT TAAGTACTCA 
AGTATGCACA AACTGGAGCC GGTGCAGAAT CAGATCCAGT CTCTGAAGAG TGACATTAAA 
CAGCTGCAGG CCGATGGAAT CAAAGCTCCT CCTCAGAGTC TGGCTGAGGC GGCCGTTCAC 
ACTGAGAACT TCATCACGGC CTTGGTTAAT GTTTTCAAGC AAAATCCATC CCCTGGTGTA 
CTGAAGGTGG GCATCACGGC TTTCTATCAG ATAGTGTCGT TTGTGTGTGA GGACACACAG 
AGACATCCAC CAACCCGCCA GTTCTTCACA TCCTGCATTG AGATCCTCGG CCAGGTCTTT 
ATTCGGGACG TCCAATCAGA ATGTAAACAC ATGCTAAAGA CTATCCTACT CAACAGACGT 
CTGTGTAACC TACTGTCACC ATACTTCACC CCGAACGCCT CTCCCTCAGA GCTGGTCTCT 
CTGTATGAAG AGGTGGTCAA CACCCTCCAC ATCGACAGCG GTGATGTCAT ATTCATGCTC 
CTCACAAAGT TTGACTTGTT TCAGTGGTTG TCGACGACCC AGCCTCCGTT CTCTGAGCGT 
AGTCGTCTGA TGACCGTCAT CCATCAGGCT CTCTGCACAT GTGGGCTGGA GCCTGAGCCC 
GAAGTCCTCA TGCCATTCAA CATCTTCTGC AAGCACTGGA CTGAACTGCT CCTCTACCAG 
TTTCCTGACC ACTACAGCGA CTTCCTCCGT CTCCTCATGC AGAGCTCTTC CGATCAGCTT 
CTTAGTCCTG ACTGTTGGAG AACGTCACTG AAAGCTCTGG GCTGTTCTCC TCAAACTAAG 
AATAAGACAA ACAAATCCCG CTCCATGCTC CTCTCCCCTC AACAGGTGGA TGAAACAATC 
GAGTGGCTCT CAAAGTACTT CCTAAAGCTC CGACTGTCTA ACGGGGACTT CAGGAGCTTT 
GGTCTTCTTT CAAAATGGGG GCCATACATT GAGGAAGTCA AGGTGTTTTG GGAAGATCTG 
ATTACCTACG TCATTGATCT GGAACTAAAC CATTGCGGCA AAGAACCTGT AGGAAGTCCA 
AGACTGCTCA AAGCTCTGGA GAACCTGCAT AGAAAAATGG CCAAACTCTT TGAGCCGTGG 
ATTTTGGTGC TTGACACTGA CAACACTAGT CATCCTCGCT GTTCTCCATG GCTTGAATCA 
GACGCCGCAG TTGCAGCGAG CTTAGTAGGC TTATATACAC GCTTGACAGA GACTTTACAT 
GAGAAATTCA AAGATCGCTT GTTGCCAGGA CAGAGAGGAG CTCTGTGGCT TCATCTCATG 
TTTTACTGTG AGACCTGCAC TTCTCCTAAA ATGCCAGAGT ATCTGCTGTA CACCTACCAC 
ACTGAGTACA GCCGTTTGCC CTGGAAGGAC CTGCATCCAG ATCAGACACT AATGAACCAG 
TTTTTCAATG TGGAGAGGGG AAGTCCAAAG AGTTGTTTTG TGTTCATGGG AGAGTTGCTG 
TGTGAGGTGA ACTGGGTTAG TGTATTGAGT GACTCTCTCA GCCCCCAGCT GAACCCCTCA 
ACCCACACAA TGGTGGTCTC CGTACTCCAC CTCATGGTGT TTCTGGCCAA AGAGGATCTC 
AGTAAACCCG AGTCCTCTGT TCTCAGTCTG CTGGACCAGT CAGCTTCTCT GCCTTGGAAT 
CTGGTGGATT TTTCTTCATA TCAAAATGTC ACGCGCTATG TCAGCACACA TTACCCAGCG 
TCCCTTGTTC TCAGTCACGA CACTTCTTCA CAGCAGGTCG TCAGATTGCT CAGAATGGCT 
GCTGGATTTG AGCCGACCAC ACAAGTGTTG CATCACAAGG ACATGACTCT AAAGTGTCAA 
GCCTTCCTTC ACCTCATGGT GCAGTTCCTG ACCTCGCTGG ATCAAAATGG GAACATCAGT 
CTGGCATCGC TAGAGACAGA AATGGAGAAA TTACTGGAGA ACATAGTCAT TTTCAACCCA 
CCTGAGACGG ACCTCCAGCA GCGACACATG GCCACCTGCA GTCTGTTTGC AGAGATGCTG 
ACTCTGCTGA ATGAGGCCGG TATCTCGACA GCAGAGGGTC TGGGCACTAA ACTGCACTGC 
TGGGTGGAGA AGCGGGCTCG AGGGCCTCTG CTTCTGCCTT TGCTAACGGC TGCGTGCAGA 
TGCCTGGCTT CAGTGCGTCA CATGACACGC ACCACCGAGG CCTGTATTCT CTCCTACTTC 
ACCGATGGAG GATGTGTGGA TCAGTACTCT GGATGGGGGC CCATCCTGGT GTCTCTTCAG 
GTTCCTGAAC TGACTGTTGA GGACTTCATT CAGGAAAGCC TAAGTCTGGG CAGCTTCCTC 
ACGCTGTACG TCTACATCCT CCAGAGGCTA AACCTGGAGC AAACCCTCCT CAATGAGAAG 
AAAACCTTAA TGCTGCTCAA TACGTGGATC AGTCAGGTGT TTCCCAGTGG TCCAGCTGAT 
GAGGCCAAGC TGTTTTTATG GTGGCATAAA TTCTTGGAGC TTCTGCAAAT CCAAGTGGAC 
CAAGAGGATG CCTGCTCACT GGACACTCTC ATTCTCACCT TAATGGCGTT TCAGAATCGT 
CTCGTTCAAC TCGGAGAGGA AAGACTAAAT TCCGGCATCC TGGGAGCCAT TGGCCTTGGG 
AGGAAATCAC CACTTTCTAA CAGGTTTCGG GTTGTGGCGC GCAGCATGTC AGCCTTTCTG 
CTGGTTCAGG TGCCCTCAGA GAATCAGCTG CGTCTTCATC CCGGGACAGA GCTGCAGCTG 
TCAGCTAAAG CCCAGCAGGC CTTGTCTGCA CTGGAGGCGA TGGCCAGTAA TAAGCAGTAT 
TCAGAGCTGC ATGTGTCTGT GAACCAGGCC TGTCAGTTCA TCAAGTACCC GAATCACTGT 
CTGCGAGACG CCAACACTCT TCTCACTCTG CTGGTCAATG CACTCTACAC TGACCTTCAT 
TACCTGGACA TCATCCGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EPG5 ( XM_009301680.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009299955.1 Danio rerio ectopic P-granules autophagy protein 5 homolog (C. elegans) (epg5), transcript variant X2, mRNA.

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CloneID	ODa45788
Clone ID Related Accession (Same CDS sequence)	XM_009301680.3 , XM_009301680.2
Accession Version	XM_009301680.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7641bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ectopic P granules protein 5 homolog isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301680.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG TGAGACCTAA AAAGGCAAAG TCCAAATCCA GTGGGAAGCC ACAGGTCAAA 
AAGGAGAGGC AGTCAGAGAG CGTCAGACAG CCCTCGGGAT CTTCTAAAGC AGTTAATGAG 
CCTGAGTTCA CTGATGTGCC GCTCAGCCTC CCTCATGTGC CTCCTGACCT CAACACAGCT 
GCCTCTCAGA CTTCACTAGA TCATCATGAC AAAGCTGAAA ATGAGCAACA GCATGTTCTC 
TTATGCGAGA AGCCTGAAGT AGTACAGAAA TCGACGTGTC ATGAAGACAA AGAGCATGAA 
TTGCCATCAC AAGATGCTGC ACCTCGGCTG GACCAAACAT CAGTGCTTGC TCCGTCCAGC 
CAAAGTGATC CCAAACCCCT CAGTGCTAAA CTTGAGCCAT TTAGTGTCCC CTCTATATAT 
CCCTCTATCC CAGCTTCCCT CTCCCAGCCT CAGCTTTCAG AGGACTCGTA CTCTATCTCC 
TACGAACTGT TAGATGGCTC TTTAATGCCA GCAGCGTCTG AGAGACTAGT AGCTCCGGCT 
GTTTTGACTC TGGCTAACCA GGAGTCCTCT CCGCCCAGCT TGGTTCCAGT GGAGGTTGTG 
GATGTGGAAA GATCTAGAGA GAGGCTTTAC CCAGAGCTGC CTAAGACCTG CCAGTCTGTG 
AAGCCCTTCA CTAGTGAGCA GCTGCGCATG TGGGAGCCCG GTTCCTGGCT GGAAAACGTG 
GAGCTGCATG AAGCCGAATT CCAGAATCTG GCTCATCAAG AGGGACATGA GCTGTACGAG 
CTGCTGCTGA GCTACTGGAG GTGCCGGAAG CAGCTGACGC AGGCCCAGAC TGAACTGCAG 
GCTGCCAACT CGGACTGCAA AAATGTGCAG AATCGCCTGT GGAGCTTCAA GGATGAAAGG 
CTTTCGTTAC AGGGTATATG TGCAGACCAG AATAAGGTGT ATGGCTACCA CTGCTACCAG 
CAGGTGACAC TGAACGAGGC TGCGTTAGCT GAGCTGAAGC ACCTGTTTTC TGCTAAAGCG 
GAAATCCTCC ATCAGACAGT GGCTCTGCAC TCATACACCT CCATCCTGTC CCGTCTGCAA 
GTGGAGTCTT ATCTCTACCG CTTGCTCAGC AGTAACCCCA CCACCAAGAG TGTGGCTGTG 
CAAGATACAA GTGCAGTGAG TCCAAAGTCT CAGCCCTCGT CTTTGCAGCC GCTGAAGGAA 
AGCATCAGTG TGCTGTTCAT CTTCACCCGA CGGGTCCTAG ATGATTCTCA GTTTCAGGCT 
GACATCCATC TTTGGCTTCA GAGGCTGGTG TCTGTGCTGC ATCAGGTCGG TTCTACAGGA 
GATCATCTAT TCATCCTAAA CCACCTGCTC TGCTGCCCGG CAGGTGTAGG GAAATGGGCT 
GTGCCATTCC TACAGATCAA AGTATTGGAT AACCCATCTG GAGTATATCA TTTCATGCAG 
GCCCTTGCTA TCCTTATGTC TCCAGCAAGG CACCGTCCAG ACTTCCTGGG ACACATGAAG 
CCCAGTGATG CAAGGGGCAA CAACAGCCTA ACAGAACCTG CATCTGGTAA CTGGACTCTA 
GTGGATGAAG GTGGAGAAGA GGACGAAGAC CCAGAAACGA GCTGGATGCT GCTGTCTGAG 
GATGATCTGG TTTCTCTGTT CTCTCAGTTT CCCTTTGATG AGCTCTTCAA ACATTTACTG 
GGCATCTGCT CTAAAGGGGA CTATCAGCCT CTAGCCACCA CAAGTCAAAA GATGATGAAG 
ATATTTGCGT TTGCCTCTTC ACTGGTTGAG TTGCTAGCAG TCGGCTTGCA GACCTACAAC 
AGAGCACGCT ATCGGCAGTT TGTGAAGAGG ATTGGTCACA TGATTCGGAT GACGCTCTGC 
TATGTGAGTG ACCACTGGGC GCAGTACATC AGTCTGACCG GTGTGAGCAG GACCACAGTG 
CAGGAGCAGT CATTTTCAAT GGAGAAACTG CAGGTGGAGT TTGACCATCT CTTCCTGAGG 
GCGGTGCTGC ATGTGCTTAA GGCCAAGAGA CTGGGCATAT GGCTGTTCAT GTCTGAGATG 
CCGTATGGGA CATTGTCCAG CTCCATGCTG TGGAAGATCT TCTACATTAT GCAGTGTGCA 
GAGACAGATG GTCTGGAGAA GCTCAGTTGT AGCTTGAGTG CTGAGGACTG TATCCAGAGG 
CTCAGAGAAT CAAGCCATCA GGAGAGGTTT CAGCTTTGGC TGTCTGAGAT AAACAGCTCA 
GATGGGATCT GCCTGTTGAC AACGTTTGCG CACATGGCCC AGCCGAGACG CACCGATGTA 
GACCCTGAGT TTGTCAGGAA CATAGTGCTA GAGATATACG AGGTTTCCTA TGTAAGTGTC 
ACCACCCGTG AGATCTTCTC TAAAGTTGGA CGGGAATTGC TTGCAGCGAT AGCGGCTGCC 
CACCCTCACA TCATCTCTGT GTTGCTGGAG AGACTGAGAG AGACCATAGA GAGAGTGGGC 
ATGGTGTCGT TGTACCTGTT TAAAGAGTTG CCCTTGCACT TGTGGATGCC AGCTCAGGAG 
GAGGTGGGGC TGATCAGAGA CTGGCTGATG AACTTCAGTC TCACTGCTGT TGAGAACAGA 
TTAGCCTGCA TTATACTAGA GGGCCTCAAC TGGGGATTTC AGCCAAATGG TTGCCTTATT 
TTACCTGCAC CTCTGCACAG TGACGTTGCC TTGTTGGTGA TTGAGGCATA TCAAAAATAT 
CTTACAGATA AACCATATGG TGGCATCCTC TCAGAAGGCA TTAAACAGGT GTCTTACCTG 
GCTAACGTGG TGAGGCTCGG CCAGACTCCA GAATCTTCCT TTAATCAGTG GGCTTGGGAT 
ATAGTTCTGA GGCTAAAGCT CCACGCCAAT GAAAGAAGCC CACAGGATGC ATGGTGCCCG 
CTTCCATCCT CCAGTGCCCC CAGTGTGCCA GAGATCACAG ACTCTCCCAT TATGCACCCA 
GTGTTCAAAG CGGTCAAAGC CGGCATCCCC ATTGGCTGTT TTCTGGCTAT TGAGATGACC 
ACCATCGGGC ACAGTTTGGA GAAGTTTTGC AGTGAAGGAA TATCTTTGCT GAAGACTCTG 
ATTCAGTCCC GTCATCTGAG AGCTGCTGTG CACATACTGG AGAACATTCT TCCTCTGGTC 
TACAACTGCC AGTTCTACCT GCTTAAGAAT GAGCAGTTTC TGAGCTGTGT CCAGCTGTTT 
TTGCAGCTGG ATAGTGGCAC TCAAGGCGTA ACCCAGCAGG TCACACAGAA GGTGGCCCAG 
CATCTGATTG GCCCCGCTAC ATGTGAGAAT ATCAAACTGC TGAACAGTGT GATCCAGGCT 
CATGTTCTGG AGAGCTCCAG GAATGGACGT GTTGGTGCAG TCGCTGTTCT GGAGTTCTGG 
CTTCAGGTTT TGACTGAACA GAGCCTCTGG TATCGAGACA GAGCTGTTCT CTTTCTTCTG 
GACCACATGT GCCGTTCTGC ATTTCTTCAC CAACAAGAGG AATGTCTCCA GAAAATACTC 
TACCAGCAGC ACAAGAGCGC TTTAGGTTAC CATGGAGACA AAGGTTTGCT CTCGTCTATT 
ATGGGCTGGA TAGTGGCAGG AAATGTTACT CCGTCTTTTA TTGAGGGCAG TTCTCTCACA 
GGAGAGGTGT GGTTTGCTTG GATGATCCTA AACATGGAGA CAATGTTCGA GGAAGATTCC 
CAGCTGCGAC GCTGTGTAGA GCATGAGCTG CTCTCAAACC CTGCGTGTAC ACCAGATCAG 
GCTTTGAAGA AAGCCCAGGT GCGTCTCAAG CTTCAGGTTG TCCCATCACT GCAGAGGCTG 
ATGATCTATC GCTGGGCTCA ACAAGCTTTA GCCACTCCTG CAGACCATCC AATCCTGCCT 
TTGGTGTGGC AGAAATTCTT CCAGCTTTAC CTCCGTCAAC CTGGACCAGA GTTTGGGCTG 
GAGGTGAAAG GTTGCATTGG CAGGCGTTAT TTCCATAGTG CGGCCCATCT GTCCTTGCTG 
AAGAGCCTCA GACAGCGGCT GGTGGAAGTG TCTGATTTCC ACCACGCCGC CAGCAAGGCT 
CTGAAAGTGC CCTGCGCTAG TGGAGATTCC GGTGATGGTC TGCTGACCCC CGGGACGCCC 
ATCACACAGT ACATGACCTC TCCTGACCTG CACACTGAGA TCGTGCGGCT CTTCACTGTG 
TTTGCCTTGT GGTTGGATGA TGAAAACCTT CAGAAGCAGG AAATTTACTT GCCCTCTTTG 
CCAAAGCAGT ATGACACCCA TCGCCTGGCC AAAATTATGC AGCGCCAGCA GGAGGTGTGG 
ATGGAGCATG TGGATATCGA GCGAGTCCAG CACGAGCTGC AGGAAGTGCT CGATCTGTGG 
TCAAAGGTCA AGAGTGAACC TGCAGTCTCA CAGAACAGCA GCTCCTCCAT CTTTACCGAC 
TTCATCAACC CTCTGGCTGC AAGGGAGCGT ATTCTCACTC ACCTGAGAAA GCATGATGCC 
CCTCAGGGGT CGCTGCTGCC AGTGCCCATG AAAGCTCCAG TGCCTGATAT TCCCACTGCG 
AGCCTAACTG ATGAGAAAAT CTCCACCACC TTAATTCAAG AGGACCTCAC TAAGCTCCAG 
CATCAAGCCA AGTTGGCAAC GGTTCGGGAA ACCCAGCAGG TTGCTCTAGA CAATGAACTG 
TTGGAGGTGT TGCCACAGCT CTACATAAAC CGGGAAGACC AGCTGTCAAT GCAGTTGGAG 
TGCCGTGGAA AAAGTGGACA GCCCTGCCAA GGTCCTGCAA ATATCACTGT TAAGTACTCA 
AGTATGCACA AACTGGAGCC GGTGCAGAAT CAGATCCAGT CTCTGAAGAG TGACATTAAA 
CAGCTGCAGG CCGATGGAAT CAAAGCTCCT CCTCAGAGTC TGGCTGAGGC GGCCGTTCAC 
ACTGAGAACT TCATCACGGC CTTGGTTAAT GTTTTCAAGC AAAATCCATC CCCTGGTGTA 
CTGAAGGTGG GCATCACGGC TTTCTATCAG ATAGTGTCGT TTGTGTGTGA GGACACACAG 
AGACATCCAC CAACCCGCCA GTTCTTCACA TCCTGCATTG AGATCCTCGG CCAGGTCTTT 
ATTCGGGACG TCCAATCAGA ATGTAAACAC ATGCTAAAGA CTATCCTACT CAACAGACGT 
CTGTGTAACC TACTGTCACC ATACTTCACC CCGAACGCCT CTCCCTCAGA GCTGGTCTCT 
CTGTATGAAG AGGTGGTCAA CACCCTCCAC ATCGACAGCG GTGATGTCAT ATTCATGCTC 
CTCACAAAGT TTGACTTGTT TCAGTGGTTG TCGACGACCC AGCCTCCGTT CTCTGAGCGT 
AGTCGTCTGA TGACCGTCAT CCATCAGGCT CTCTGCACAT GTGGGCTGGA GCCTGAGCCC 
GAAGTCCTCA TGCCATTCAA CATCTTCTGC AAGCACTGGA CTGAACTGCT CCTCTACCAG 
TTTCCTGACC ACTACAGCGA CTTCCTCCGT CTCCTCATGC AGAGCTCTTC CGATCAGCTT 
CTTAGTCCTG ACTGTTGGAG AACGTCACTG AAAGCTCTGG GCTGTTCTCC TCAAACTAAG 
AATAAGACAA ACAAATCCCG CTCCATGCTC CTCTCCCCTC AACAGGTGGA TGAAACAATC 
GAGTGGCTCT CAAAGTACTT CCTAAAGCTC CGACTGTCTA ACGGGGACTT CAGGAGCTTT 
GGTCTTCTTT CAAAATGGGG GCCATACATT GAGGAAGTCA AGGTGTTTTG GGAAGATCTG 
ATTACCTACG TCATTGATCT GGAACTAAAC CATTGCGGCA AAGAACCTGT AGGAAGTCCA 
AGACTGCTCA AAGCTCTGGA GAACCTGCAT AGAAAAATGG CCAAACTCTT TGAGCCGTGG 
ATTTTGGTGC TTGACACTGA CAACACTAGT CATCCTCGCT GTTCTCCATG GCTTGAATCA 
GACGCCGCAG TTGCAGCGAG CTTAGTAGGC TTATATACAC GCTTGACAGA GACTTTACAT 
GAGAAATTCA AAGATCGCTT GTTGCCAGGA CAGAGAGGAG CTCTGTGGCT TCATCTCATG 
TTTTACTGTG AGACCTGCAC TTCTCCTAAA ATGCCAGAGT ATCTGCTGTA CACCTACCAC 
ACTGAGTACA GCCGTTTGCC CTGGAAGGAC CTGCATCCAG ATCAGACACT AATGAACCAG 
TTTTTCAATG TGGAGAGGGG AAGTCCAAAG AGTTGTTTTG TGTTCATGGG AGAGTTGCTG 
TGTGAGGTGA ACTGGGTTAG TGTATTGAGT GACTCTCTCA GCCCCCAGCT GAACCCCTCA 
ACCCACACAA TGGTGGTCTC CGTACTCCAC CTCATGGTGT TTCTGGCCAA AGAGGATCTC 
AGTAAACCCG AGTCCTCTGT TCTCAGTCTG CTGGACCAGT CAGCTTCTCT GCCTTGGAAT 
CTGGTGGATT TTTCTTCATA TCAAAATGTC ACGCGCTATG TCAGCACACA TTACCCAGCG 
TCCCTTGTTC TCAGTCACGA CACTTCTTCA CAGCAGGTCG TCAGATTGCT CAGAATGGCT 
GCTGGATTTG AGCCGACCAC ACAAGTGTTG CATCACAAGG ACATGACTCT AAAGTGTCAA 
GCCTTCCTTC ACCTCATGGT GCAGTTCCTG ACCTCGCTGG ATCAAAATGG GAACATCAGT 
CTGGCATCGC TAGAGACAGA AATGGAGAAA TTACTGGAGA ACATAGTCAT TTTCAACCCA 
CCTGAGACGG ACCTCCAGCA GCGACACATG GCCACCTGCA GTCTGTTTGC AGAGATGCTG 
ACTCTGCTGA ATGAGGCCGG TATCTCGACA GCAGAGGGTC TGGGCACTAA ACTGCACTGC 
TGGGTGGAGA AGCGGGCTCG AGGGCCTCTG CTTCTGCCTT TGCTAACGGC TGCGTGCAGA 
TGCCTGGCTT CAGTGCGTCA CATGACACGC ACCACCGAGG CCTGTATTCT CTCCTACTTC 
ACCGATGGAG GATGTGTGGA TCAGTACTCT GGATGGGGGC CCATCCTGGT GTCTCTTCAG 
GTTCCTGAAC TGACTGTTGA GGACTTCATT CAGGAAAGCC TAAGTCTGGG CAGCTTCCTC 
ACGCTGTACG TCTACATCCT CCAGAGGCTA AACCTGGAGC AAACCCTCCT CAATGAGAAG 
AAAACCTTAA TGCTGCTCAA TACGTGGATC AGTCAGGTGT TTCCCAGTGG TCCAGCTGAT 
GAGGCCAAGC TGTTTTTATG GTGGCATAAA TTCTTGGAGC TTCTGCAAAT CCAAGTGGAC 
CAAGAGGATG CCTGCTCACT GGACACTCTC ATTCTCACCT TAATGGCGTT TCAGAATCGT 
CTCGTTCAAC TCGGAGAGGA AAGACTAAAT TCCGGCATCC TGGGAGCCAT TGGCCTTGGG 
AGGAAATCAC CACTTTCTAA CAGGTTTCGG GTTGTGGCGC GCAGCATGTC AGCCTTTCTG 
CTGGTTCAGG TGCCCTCAGA GAATCAGCTG CGTCTTCATC CCGGGACAGA GCTGCAGCTG 
TCAGCTAAAG CCCAGCAGGC CTTGTCTGCA CTGGAGGCGA TGGCCAGTAA TAAGCAGTAT 
TCAGAGCTGC ATGTGTCTGT GAACCAGGCC TGTCAGTTCA TCAAGTACCC GAATCACTGT 
CTGCGAGACG CCAACACTCT TCTCACTCTG CTGGTCAATG CACTCTACAC TGACCTTCAT 
TACCTGGACA TCATCCGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299955.1
CDS	192..7832
Translation	MEAVRPKKAKSKSSGKPQVKKERQSESVRQPSGSSKAVNEPEFTDVPLSLPHVPPDLNTAASQTSLDHHDKAENEQQHVLLCEKPEVVQKSTCHEDKEHELPSQDAAPRLDQTSVLAPSSQSDPKPLSAKLEPFSVPSIYPSIPASLSQPQLSEDSYSISYELLDGSLMPAASERLVAPAVLTLANQESSPPSLVPVEVVDVERSRERLYPELPKTCQSVKPFTSEQLRMWEPGSWLENVELHEAEFQNLAHQEGHELYELLLSYWRCRKQLTQAQTELQAANSDCKNVQNRLWSFKDERLSLQGICADQNKVYGYHCYQQVTLNEAALAELKHLFSAKAEILHQTVALHSYTSILSRLQVESYLYRLLSSNPTTKSVAVQDTSAVSPKSQPSSLQPLKESISVLFIFTRRVLDDSQFQADIHLWLQRLVSVLHQVGSTGDHLFILNHLLCCPAGVGKWAVPFLQIKVLDNPSGVYHFMQALAILMSPARHRPDFLGHMKPSDARGNNSLTEPASGNWTLVDEGGEEDEDPETSWMLLSEDDLVSLFSQFPFDELFKHLLGICSKGDYQPLATTSQKMMKIFAFASSLVELLAVGLQTYNRARYRQFVKRIGHMIRMTLCYVSDHWAQYISLTGVSRTTVQEQSFSMEKLQVEFDHLFLRAVLHVLKAKRLGIWLFMSEMPYGTLSSSMLWKIFYIMQCAETDGLEKLSCSLSAEDCIQRLRESSHQERFQLWLSEINSSDGICLLTTFAHMAQPRRTDVDPEFVRNIVLEIYEVSYVSVTTREIFSKVGRELLAAIAAAHPHIISVLLERLRETIERVGMVSLYLFKELPLHLWMPAQEEVGLIRDWLMNFSLTAVENRLACIILEGLNWGFQPNGCLILPAPLHSDVALLVIEAYQKYLTDKPYGGILSEGIKQVSYLANVVRLGQTPESSFNQWAWDIVLRLKLHANERSPQDAWCPLPSSSAPSVPEITDSPIMHPVFKAVKAGIPIGCFLAIEMTTIGHSLEKFCSEGISLLKTLIQSRHLRAAVHILENILPLVYNCQFYLLKNEQFLSCVQLFLQLDSGTQGVTQQVTQKVAQHLIGPATCENIKLLNSVIQAHVLESSRNGRVGAVAVLEFWLQVLTEQSLWYRDRAVLFLLDHMCRSAFLHQQEECLQKILYQQHKSALGYHGDKGLLSSIMGWIVAGNVTPSFIEGSSLTGEVWFAWMILNMETMFEEDSQLRRCVEHELLSNPACTPDQALKKAQVRLKLQVVPSLQRLMIYRWAQQALATPADHPILPLVWQKFFQLYLRQPGPEFGLEVKGCIGRRYFHSAAHLSLLKSLRQRLVEVSDFHHAASKALKVPCASGDSGDGLLTPGTPITQYMTSPDLHTEIVRLFTVFALWLDDENLQKQEIYLPSLPKQYDTHRLAKIMQRQQEVWMEHVDIERVQHELQEVLDLWSKVKSEPAVSQNSSSSIFTDFINPLAARERILTHLRKHDAPQGSLLPVPMKAPVPDIPTASLTDEKISTTLIQEDLTKLQHQAKLATVRETQQVALDNELLEVLPQLYINREDQLSMQLECRGKSGQPCQGPANITVKYSSMHKLEPVQNQIQSLKSDIKQLQADGIKAPPQSLAEAAVHTENFITALVNVFKQNPSPGVLKVGITAFYQIVSFVCEDTQRHPPTRQFFTSCIEILGQVFIRDVQSECKHMLKTILLNRRLCNLLSPYFTPNASPSELVSLYEEVVNTLHIDSGDVIFMLLTKFDLFQWLSTTQPPFSERSRLMTVIHQALCTCGLEPEPEVLMPFNIFCKHWTELLLYQFPDHYSDFLRLLMQSSSDQLLSPDCWRTSLKALGCSPQTKNKTNKSRSMLLSPQQVDETIEWLSKYFLKLRLSNGDFRSFGLLSKWGPYIEEVKVFWEDLITYVIDLELNHCGKEPVGSPRLLKALENLHRKMAKLFEPWILVLDTDNTSHPRCSPWLESDAAVAASLVGLYTRLTETLHEKFKDRLLPGQRGALWLHLMFYCETCTSPKMPEYLLYTYHTEYSRLPWKDLHPDQTLMNQFFNVERGSPKSCFVFMGELLCEVNWVSVLSDSLSPQLNPSTHTMVVSVLHLMVFLAKEDLSKPESSVLSLLDQSASLPWNLVDFSSYQNVTRYVSTHYPASLVLSHDTSSQQVVRLLRMAAGFEPTTQVLHHKDMTLKCQAFLHLMVQFLTSLDQNGNISLASLETEMEKLLENIVIFNPPETDLQQRHMATCSLFAEMLTLLNEAGISTAEGLGTKLHCWVEKRARGPLLLPLLTAACRCLASVRHMTRTTEACILSYFTDGGCVDQYSGWGPILVSLQVPELTVEDFIQESLSLGSFLTLYVYILQRLNLEQTLLNEKKTLMLLNTWISQVFPSGPADEAKLFLWWHKFLELLQIQVDQEDACSLDTLILTLMAFQNRLVQLGEERLNSGILGAIGLGRKSPLSNRFRVVARSMSAFLLVQVPSENQLRLHPGTELQLSAKAQQALSALEAMASNKQYSELHVSVNQACQFIKYPNHCLRDANTLLTLLVNALYTDLHYLDIIR

Target ORF information:

RefSeq Version	XM_009301680.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ectopic P-granules autophagy protein 5 homolog (C. elegans) (epg5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301680.2

ORF Insert Sequence:

ATGGAGGCTG TGAGACCTAA AAAGGCAAAG TCCAAATCCA GTGGGAAGCC ACAGGTCAAA 
AAGGAGAGGC AGTCAGAGAG CGTCAGACAG CCCTCGGGAT CTTCTAAAGC AGTTAATGAG 
CCTGAGTTCA CTGATGTGCC GCTCAGCCTC CCTCATGTGC CTCCTGACCT CAACACAGCT 
GCCTCTCAGA CTTCACTAGA TCATCATGAC AAAGCTGAAA ATGAGCAACA GCATGTTCTC 
TTATGCGAGA AGCCTGAAGT AGTACAGAAA TCGACGTGTC ATGAAGACAA AGAGCATGAA 
TTGCCATCAC AAGATGCTGC ACCTCGGCTG GACCAAACAT CAGTGCTTGC TCCGTCCAGC 
CAAAGTGATC CCAAACCCCT CAGTGCTAAA CTTGAGCCAT TTAGTGTCCC CTCTATATAT 
CCCTCTATCC CAGCTTCCCT CTCCCAGCCT CAGCTTTCAG AGGACTCGTA CTCTATCTCC 
TACGAACTGT TAGATGGCTC TTTAATGCCA GCAGCGTCTG AGAGACTAGT AGCTCCGGCT 
GTTTTGACTC TGGCTAACCA GGAGTCCTCT CCGCCCAGCT TGGTTCCAGT GGAGGTTGTG 
GATGTGGAAA GATCTAGAGA GAGGCTTTAC CCAGAGCTGC CTAAGACCTG CCAGTCTGTG 
AAGCCCTTCA CTAGTGAGCA GCTGCGCATG TGGGAGCCCG GTTCCTGGCT GGAAAACGTG 
GAGCTGCATG AAGCCGAATT CCAGAATCTG GCTCATCAAG AGGGACATGA GCTGTACGAG 
CTGCTGCTGA GCTACTGGAG GTGCCGGAAG CAGCTGACGC AGGCCCAGAC TGAACTGCAG 
GCTGCCAACT CGGACTGCAA AAATGTGCAG AATCGCCTGT GGAGCTTCAA GGATGAAAGG 
CTTTCGTTAC AGGGTATATG TGCAGACCAG AATAAGGTGT ATGGCTACCA CTGCTACCAG 
CAGGTGACAC TGAACGAGGC TGCGTTAGCT GAGCTGAAGC ACCTGTTTTC TGCTAAAGCG 
GAAATCCTCC ATCAGACAGT GGCTCTGCAC TCATACACCT CCATCCTGTC CCGTCTGCAA 
GTGGAGTCTT ATCTCTACCG CTTGCTCAGC AGTAACCCCA CCACCAAGAG TGTGGCTGTG 
CAAGATACAA GTGCAGTGAG TCCAAAGTCT CAGCCCTCGT CTTTGCAGCC GCTGAAGGAA 
AGCATCAGTG TGCTGTTCAT CTTCACCCGA CGGGTCCTAG ATGATTCTCA GTTTCAGGCT 
GACATCCATC TTTGGCTTCA GAGGCTGGTG TCTGTGCTGC ATCAGGTCGG TTCTACAGGA 
GATCATCTAT TCATCCTAAA CCACCTGCTC TGCTGCCCGG CAGGTGTAGG GAAATGGGCT 
GTGCCATTCC TACAGATCAA AGTATTGGAT AACCCATCTG GAGTATATCA TTTCATGCAG 
GCCCTTGCTA TCCTTATGTC TCCAGCAAGG CACCGTCCAG ACTTCCTGGG ACACATGAAG 
CCCAGTGATG CAAGGGGCAA CAACAGCCTA ACAGAACCTG CATCTGGTAA CTGGACTCTA 
GTGGATGAAG GTGGAGAAGA GGACGAAGAC CCAGAAACGA GCTGGATGCT GCTGTCTGAG 
GATGATCTGG TTTCTCTGTT CTCTCAGTTT CCCTTTGATG AGCTCTTCAA ACATTTACTG 
GGCATCTGCT CTAAAGGGGA CTATCAGCCT CTAGCCACCA CAAGTCAAAA GATGATGAAG 
ATATTTGCGT TTGCCTCTTC ACTGGTTGAG TTGCTAGCAG TCGGCTTGCA GACCTACAAC 
AGAGCACGCT ATCGGCAGTT TGTGAAGAGG ATTGGTCACA TGATTCGGAT GACGCTCTGC 
TATGTGAGTG ACCACTGGGC GCAGTACATC AGTCTGACCG GTGTGAGCAG GACCACAGTG 
CAGGAGCAGT CATTTTCAAT GGAGAAACTG CAGGTGGAGT TTGACCATCT CTTCCTGAGG 
GCGGTGCTGC ATGTGCTTAA GGCCAAGAGA CTGGGCATAT GGCTGTTCAT GTCTGAGATG 
CCGTATGGGA CATTGTCCAG CTCCATGCTG TGGAAGATCT TCTACATTAT GCAGTGTGCA 
GAGACAGATG GTCTGGAGAA GCTCAGTTGT AGCTTGAGTG CTGAGGACTG TATCCAGAGG 
CTCAGAGAAT CAAGCCATCA GGAGAGGTTT CAGCTTTGGC TGTCTGAGAT AAACAGCTCA 
GATGGGATCT GCCTGTTGAC AACGTTTGCG CACATGGCCC AGCCGAGACG CACCGATGTA 
GACCCTGAGT TTGTCAGGAA CATAGTGCTA GAGATATACG AGGTTTCCTA TGTAAGTGTC 
ACCACCCGTG AGATCTTCTC TAAAGTTGGA CGGGAATTGC TTGCAGCGAT AGCGGCTGCC 
CACCCTCACA TCATCTCTGT GTTGCTGGAG AGACTGAGAG AGACCATAGA GAGAGTGGGC 
ATGGTGTCGT TGTACCTGTT TAAAGAGTTG CCCTTGCACT TGTGGATGCC AGCTCAGGAG 
GAGGTGGGGC TGATCAGAGA CTGGCTGATG AACTTCAGTC TCACTGCTGT TGAGAACAGA 
TTAGCCTGCA TTATACTAGA GGGCCTCAAC TGGGGATTTC AGCCAAATGG TTGCCTTATT 
TTACCTGCAC CTCTGCACAG TGACGTTGCC TTGTTGGTGA TTGAGGCATA TCAAAAATAT 
CTTACAGATA AACCATATGG TGGCATCCTC TCAGAAGGCA TTAAACAGGT GTCTTACCTG 
GCTAACGTGG TGAGGCTCGG CCAGACTCCA GAATCTTCCT TTAATCAGTG GGCTTGGGAT 
ATAGTTCTGA GGCTAAAGCT CCACGCCAAT GAAAGAAGCC CACAGGATGC ATGGTGCCCG 
CTTCCATCCT CCAGTGCCCC CAGTGTGCCA GAGATCACAG ACTCTCCCAT TATGCACCCA 
GTGTTCAAAG CGGTCAAAGC CGGCATCCCC ATTGGCTGTT TTCTGGCTAT TGAGATGACC 
ACCATCGGGC ACAGTTTGGA GAAGTTTTGC AGTGAAGGAA TATCTTTGCT GAAGACTCTG 
ATTCAGTCCC GTCATCTGAG AGCTGCTGTG CACATACTGG AGAACATTCT TCCTCTGGTC 
TACAACTGCC AGTTCTACCT GCTTAAGAAT GAGCAGTTTC TGAGCTGTGT CCAGCTGTTT 
TTGCAGCTGG ATAGTGGCAC TCAAGGCGTA ACCCAGCAGG TCACACAGAA GGTGGCCCAG 
CATCTGATTG GCCCCGCTAC ATGTGAGAAT ATCAAACTGC TGAACAGTGT GATCCAGGCT 
CATGTTCTGG AGAGCTCCAG GAATGGACGT GTTGGTGCAG TCGCTGTTCT GGAGTTCTGG 
CTTCAGGTTT TGACTGAACA GAGCCTCTGG TATCGAGACA GAGCTGTTCT CTTTCTTCTG 
GACCACATGT GCCGTTCTGC ATTTCTTCAC CAACAAGAGG AATGTCTCCA GAAAATACTC 
TACCAGCAGC ACAAGAGCGC TTTAGGTTAC CATGGAGACA AAGGTTTGCT CTCGTCTATT 
ATGGGCTGGA TAGTGGCAGG AAATGTTACT CCGTCTTTTA TTGAGGGCAG TTCTCTCACA 
GGAGAGGTGT GGTTTGCTTG GATGATCCTA AACATGGAGA CAATGTTCGA GGAAGATTCC 
CAGCTGCGAC GCTGTGTAGA GCATGAGCTG CTCTCAAACC CTGCGTGTAC ACCAGATCAG 
GCTTTGAAGA AAGCCCAGGT GCGTCTCAAG CTTCAGGTTG TCCCATCACT GCAGAGGCTG 
ATGATCTATC GCTGGGCTCA ACAAGCTTTA GCCACTCCTG CAGACCATCC AATCCTGCCT 
TTGGTGTGGC AGAAATTCTT CCAGCTTTAC CTCCGTCAAC CTGGACCAGA GTTTGGGCTG 
GAGGTGAAAG GTTGCATTGG CAGGCGTTAT TTCCATAGTG CGGCCCATCT GTCCTTGCTG 
AAGAGCCTCA GACAGCGGCT GGTGGAAGTG TCTGATTTCC ACCACGCCGC CAGCAAGGCT 
CTGAAAGTGC CCTGCGCTAG TGGAGATTCC GGTGATGGTC TGCTGACCCC CGGGACGCCC 
ATCACACAGT ACATGACCTC TCCTGACCTG CACACTGAGA TCGTGCGGCT CTTCACTGTG 
TTTGCCTTGT GGTTGGATGA TGAAAACCTT CAGAAGCAGG AAATTTACTT GCCCTCTTTG 
CCAAAGCAGT ATGACACCCA TCGCCTGGCC AAAATTATGC AGCGCCAGCA GGAGGTGTGG 
ATGGAGCATG TGGATATCGA GCGAGTCCAG CACGAGCTGC AGGAAGTGCT CGATCTGTGG 
TCAAAGGTCA AGAGTGAACC TGCAGTCTCA CAGAACAGCA GCTCCTCCAT CTTTACCGAC 
TTCATCAACC CTCTGGCTGC AAGGGAGCGT ATTCTCACTC ACCTGAGAAA GCATGATGCC 
CCTCAGGGGT CGCTGCTGCC AGTGCCCATG AAAGCTCCAG TGCCTGATAT TCCCACTGCG 
AGCCTAACTG ATGAGAAAAT CTCCACCACC TTAATTCAAG AGGACCTCAC TAAGCTCCAG 
CATCAAGCCA AGTTGGCAAC GGTTCGGGAA ACCCAGCAGG TTGCTCTAGA CAATGAACTG 
TTGGAGGTGT TGCCACAGCT CTACATAAAC CGGGAAGACC AGCTGTCAAT GCAGTTGGAG 
TGCCGTGGAA AAAGTGGACA GCCCTGCCAA GGTCCTGCAA ATATCACTGT TAAGTACTCA 
AGTATGCACA AACTGGAGCC GGTGCAGAAT CAGATCCAGT CTCTGAAGAG TGACATTAAA 
CAGCTGCAGG CCGATGGAAT CAAAGCTCCT CCTCAGAGTC TGGCTGAGGC GGCCGTTCAC 
ACTGAGAACT TCATCACGGC CTTGGTTAAT GTTTTCAAGC AAAATCCATC CCCTGGTGTA 
CTGAAGGTGG GCATCACGGC TTTCTATCAG ATAGTGTCGT TTGTGTGTGA GGACACACAG 
AGACATCCAC CAACCCGCCA GTTCTTCACA TCCTGCATTG AGATCCTCGG CCAGGTCTTT 
ATTCGGGACG TCCAATCAGA ATGTAAACAC ATGCTAAAGA CTATCCTACT CAACAGACGT 
CTGTGTAACC TACTGTCACC ATACTTCACC CCGAACGCCT CTCCCTCAGA GCTGGTCTCT 
CTGTATGAAG AGGTGGTCAA CACCCTCCAC ATCGACAGCG GTGATGTCAT ATTCATGCTC 
CTCACAAAGT TTGACTTGTT TCAGTGGTTG TCGACGACCC AGCCTCCGTT CTCTGAGCGT 
AGTCGTCTGA TGACCGTCAT CCATCAGGCT CTCTGCACAT GTGGGCTGGA GCCTGAGCCC 
GAAGTCCTCA TGCCATTCAA CATCTTCTGC AAGCACTGGA CTGAACTGCT CCTCTACCAG 
TTTCCTGACC ACTACAGCGA CTTCCTCCGT CTCCTCATGC AGAGCTCTTC CGATCAGCTT 
CTTAGTCCTG ACTGTTGGAG AACGTCACTG AAAGCTCTGG GCTGTTCTCC TCAAACTAAG 
AATAAGACAA ACAAATCCCG CTCCATGCTC CTCTCCCCTC AACAGGTGGA TGAAACAATC 
GAGTGGCTCT CAAAGTACTT CCTAAAGCTC CGACTGTCTA ACGGGGACTT CAGGAGCTTT 
GGTCTTCTTT CAAAATGGGG GCCATACATT GAGGAAGTCA AGGTGTTTTG GGAAGATCTG 
ATTACCTACG TCATTGATCT GGAACTAAAC CATTGCGGCA AAGAACCTGT AGGAAGTCCA 
AGACTGCTCA AAGCTCTGGA GAACCTGCAT AGAAAAATGG CCAAACTCTT TGAGCCGTGG 
ATTTTGGTGC TTGACACTGA CAACACTAGT CATCCTCGCT GTTCTCCATG GCTTGAATCA 
GACGCCGCAG TTGCAGCGAG CTTAGTAGGC TTATATACAC GCTTGACAGA GACTTTACAT 
GAGAAATTCA AAGATCGCTT GTTGCCAGGA CAGAGAGGAG CTCTGTGGCT TCATCTCATG 
TTTTACTGTG AGACCTGCAC TTCTCCTAAA ATGCCAGAGT ATCTGCTGTA CACCTACCAC 
ACTGAGTACA GCCGTTTGCC CTGGAAGGAC CTGCATCCAG ATCAGACACT AATGAACCAG 
TTTTTCAATG TGGAGAGGGG AAGTCCAAAG AGTTGTTTTG TGTTCATGGG AGAGTTGCTG 
TGTGAGGTGA ACTGGGTTAG TGTATTGAGT GACTCTCTCA GCCCCCAGCT GAACCCCTCA 
ACCCACACAA TGGTGGTCTC CGTACTCCAC CTCATGGTGT TTCTGGCCAA AGAGGATCTC 
AGTAAACCCG AGTCCTCTGT TCTCAGTCTG CTGGACCAGT CAGCTTCTCT GCCTTGGAAT 
CTGGTGGATT TTTCTTCATA TCAAAATGTC ACGCGCTATG TCAGCACACA TTACCCAGCG 
TCCCTTGTTC TCAGTCACGA CACTTCTTCA CAGCAGGTCG TCAGATTGCT CAGAATGGCT 
GCTGGATTTG AGCCGACCAC ACAAGTGTTG CATCACAAGG ACATGACTCT AAAGTGTCAA 
GCCTTCCTTC ACCTCATGGT GCAGTTCCTG ACCTCGCTGG ATCAAAATGG GAACATCAGT 
CTGGCATCGC TAGAGACAGA AATGGAGAAA TTACTGGAGA ACATAGTCAT TTTCAACCCA 
CCTGAGACGG ACCTCCAGCA GCGACACATG GCCACCTGCA GTCTGTTTGC AGAGATGCTG 
ACTCTGCTGA ATGAGGCCGG TATCTCGACA GCAGAGGGTC TGGGCACTAA ACTGCACTGC 
TGGGTGGAGA AGCGGGCTCG AGGGCCTCTG CTTCTGCCTT TGCTAACGGC TGCGTGCAGA 
TGCCTGGCTT CAGTGCGTCA CATGACACGC ACCACCGAGG CCTGTATTCT CTCCTACTTC 
ACCGATGGAG GATGTGTGGA TCAGTACTCT GGATGGGGGC CCATCCTGGT GTCTCTTCAG 
GTTCCTGAAC TGACTGTTGA GGACTTCATT CAGGAAAGCC TAAGTCTGGG CAGCTTCCTC 
ACGCTGTACG TCTACATCCT CCAGAGGCTA AACCTGGAGC AAACCCTCCT CAATGAGAAG 
AAAACCTTAA TGCTGCTCAA TACGTGGATC AGTCAGGTGT TTCCCAGTGG TCCAGCTGAT 
GAGGCCAAGC TGTTTTTATG GTGGCATAAA TTCTTGGAGC TTCTGCAAAT CCAAGTGGAC 
CAAGAGGATG CCTGCTCACT GGACACTCTC ATTCTCACCT TAATGGCGTT TCAGAATCGT 
CTCGTTCAAC TCGGAGAGGA AAGACTAAAT TCCGGCATCC TGGGAGCCAT TGGCCTTGGG 
AGGAAATCAC CACTTTCTAA CAGGTTTCGG GTTGTGGCGC GCAGCATGTC AGCCTTTCTG 
CTGGTTCAGG TGCCCTCAGA GAATCAGCTG CGTCTTCATC CCGGGACAGA GCTGCAGCTG 
TCAGCTAAAG CCCAGCAGGC CTTGTCTGCA CTGGAGGCGA TGGCCAGTAA TAAGCAGTAT 
TCAGAGCTGC ATGTGTCTGT GAACCAGGCC TGTCAGTTCA TCAAGTACCC GAATCACTGT 
CTGCGAGACG CCAACACTCT TCTCACTCTG CTGGTCAATG CACTCTACAC TGACCTTCAT 
TACCTGGACA TCATCCGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium.
Briefings in bioinformatics12(5)449-62(2011 Sep)
Gaudet P,Livstone MS,Lewis SE,Thomas PD

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EPG5 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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