gRNAs
The following lig4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lig4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ODa00467 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001103123.1 | |
| Accession Version | NM_001103123.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2730bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2020-01-04 | |
| Organism | Danio rerio(zebrafish) | |
| Product | DNA ligase 4 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC150236.1. On Sep 5, 2007 this sequence version replaced XM_692920.2. ##Evidence-Data-START## Transcript exon combination :: BC150236.1, DV600712.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGAAAGTG TCTCAAAAAG TGATGCCAAA CCACAGGCAT TTGTTGCTGC GCAAGTTCCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001096593.1 |
| CDS | 35..2764 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001103123.1 |
1 | ATGGAAAGTG TCTCAAAAAG TGATGCCAAA CCACAGGCAT TTGTTGCTGC GCAAGTTCCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ODa05824 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005159958.4 , XM_005159958.3 | |
| Accession Version | XM_005159958.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2730bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-06-14 | |
| Organism | Danio rerio(zebrafish) | |
| Product | DNA ligase 4 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159958.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAAAGTG TCTCAAAAAG TGATGCCAAA CCACAGGCAT TTGTTGCTGC GCAAGTTCCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005160015.1 |
| CDS | 153..2882 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005159958.4 |
1 | ATGGAAAGTG TCTCAAAAAG TGATGCCAAA CCACAGGCAT TTGTTGCTGC GCAAGTTCCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ODa05824 | |
| Clone ID Related Accession (Same CDS sequence) | XM_005159958.4 , XM_005159958.3 | |
| Accession Version | XM_005159958.3 | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2730bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-06-23 | |
| Organism | Danio rerio(Zebrafish) | |
| Product | DNA ligase 4 isoform X1 | |
| Comment | MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159958.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAAAGTG TCTCAAAAAG TGATGCCAAA CCACAGGCAT TTGTTGCTGC GCAAGTTCCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_005160015.1 |
| CDS | 149..2878 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_005159958.3 |
1 | ATGGAAAGTG TCTCAAAAAG TGATGCCAAA CCACAGGCAT TTGTTGCTGC GCAAGTTCCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
PCR artifact in testing for homologous recombination in genomic editing in zebrafish. |
Expanding CRISPR/Cas9 Genome Editing Capacity in Zebrafish Using SaCas9. |
Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database. |
