mylka cDNA ORF clone, Danio rerio(zebrafish)

The following mylka gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mylka cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa60285 XM_017357739.1
Latest version!
Danio rerio myosin, light chain kinase a (mylka), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa60286 XM_017357740.1
Latest version!
Danio rerio myosin, light chain kinase a (mylka), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $1021.30
$1459.00
ODa45772 XM_009304445.1
Latest version!
Danio rerio myosin, light chain kinase a (mylka), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa45773 XM_009304446.1
Latest version!
Danio rerio myosin, light chain kinase a (mylka), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa60285
    Clone ID Related Accession (Same CDS sequence) XM_017357739.1
    Accession Version XM_017357739.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5571bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product myosin light chain kinase, smooth muscle isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGCAGCT CTCAGCCTGT GATGTTAGGA TTCATGCCAG CAAAGTTTGG TGCCGACAGG 
    GAAAACACGT TAAACGCAGA GTGGAGTCGG GACAGCGGGG TCTGGACGCG CTCTCGACAC
    TGCACTCCGG TGAATGACAC TAATCTGCAC TGCTGTCTAC ATGGAATGAT GGCCGATGTT
    CAGTTTGTTA CAGGATCTCA TGTCTCCAAG ACAGCTCTGA CTCTGGGACG AGGTTCCCCC
    AGCAAAGAGC CCCCCGTTTT TACCCTCCCG CCCCGCAATA CCCGTGTCTG CCAAGGGGGA
    ACTGCGCGCC TTGAGGGGAA GGTACGTGGT TTCCCAGAGC CTCAGGTATG CTGGTTTAGG
    AATGGCAAGC TACTGATAGC AGGAGATCAT TATTCTATGG AACAGAGTGT CCGTGGAACA
    TTTAGTCTGG TGGTCCAGGA GGTAGAGGAT GCCGATGGAG GACGTTACAC CTGTGAAGCT
    GTTAACGATG CTGGGAGCTG CCAGGTTACT GTGGAGATCA TTGTGGAAGG AAACACAGGA
    AAGAAATACA GTCTTCCTTC CACAAACAGA ACAGGATCTA TGGGAGCTCC CGCAGTAGAA
    AACAGAGCCA GTATCTGGGG TGAAAGCCCC CCAAAGTTTG TGGCTAAACC TTCTCGCCTG
    TATCTCAAAG TGGGACAAAC TGGCAAATTT TCGGCCAAGA TCACAGGACG ACCCCAGCCA
    CTGGTGCAGT GGTACAAGGG AGAAGAGATG ATCCAGCAAT CAGATCACTA TAACATGTTT
    GAGAGGTCAG GAATGCATTT CCTGGAGATC CGCAATGTGT TCTCTGAGGA TGCTGGCATG
    TACACCTGTC TGGTGGCCAA CAGTGCTGGA AAAGCCTCTG CAGCCGCTGA ACTCATTATG
    CAAGATGGAG ACACCGAGAC AGTCATCATG GCCAAACCAT CTCACAATCC CTCCAAGTCA
    TTCATGCTCA AAGACACTTC CAACACTCTT GCCAACGAAC AGAAAACTTC CAGCACATCC
    ATCGAAACCA TAATCACAGA AACACCCACA CCGCTGTCCA CTACTACTGT TCCCACTGTC
    CCATCCATGA ACAGAGGAGA AACAACAATA GTAACATCAT CACTTCACTC ACAACCTATC
    AGCAGGTCCA CAGATGGAAG ATCAGCAACT GGCCAAAATA CTTCAAACTT AGTCAATCTA
    AGAAGTCAAA ACAGTGTTAA GAATGAGACG CATGGCACAA AGAGAGTTTT GGGTATGTTC
    AGAGCTCCGG GACAGATGCC ACAATCATCA CAGATAGAAT CCAGCACTCG CCAACAAACC
    AAAGAAACCA GAATGGATGT GCTAGAAACT CCACCCAGTT TTGACTCGCA GCCCCAGAGC
    CAAGAGGTCC TGCAGGGTGA AAAGGTCACT TTCAAATGCC AAGTGTCCGG GTCTCCAGTG
    CCTGCAGTGA GCTGGCTGAA AGATGGAGCT CCTCTCAGAC AGAGATCAGA TGTCAGCACT
    CGACAGGAGG CCGGCCTGCA TGTTCTGTGT CTGGACAATG CTCACATAAC TGACACGGGA
    CAGTATGGCT GCACTGCTGC CAACAGAAGA GGAAACACCG CGGCCCACTG GAGTCTGACC
    GTCAAGCGTG TGCGAGTGGA TGCGAAGCCC CCAGTGTTCA GCTCTGCTCT GAAGGATTGT
    TCGGTGTGTG AAGGACAGGA ATTTACCCTG CAGTGTTCAG TGGAGGGCAA ACCACAGCCT
    GAGCTCTCCT GGCTGCACAA CGATAAGCCC ATTCCATTCT CCCATACCAT GTTTGACAAC
    AGTAAAGCTC AGCTGAGAGT TCAGGCGGCT GTATTGGAGG ATGCAGGCGT TTATTCGTGT
    GTAGCAGTGA ATGAGCATGG CAGAGCGGTG TGTACAGCCC ATGTGCTGGT TAAAGCAAGG
    CCAAGGGTGA AAAGGTCTGC TGCTCCTCCT GTTACTGATC TGAGGAGAAC ATCTGCTCCG
    CTGTTCCTCA AAGGTCTACA GGACCTCAGG GTGATGGACG GCAGTCAGGT CATCATGACC
    GTGGAAGTGA CAGGAAACCC TGCACCTGAG GTGGTCTGGC TGCATAATGG AAAAGAGATC
    CAGGAGTCAG AGGACTTTCA CTTTGAAAAG AAGGGCGGTC AGTATACACT CTTCATACAG
    GAGGTGTTTC CTGAGGACAC GGGGACATAC ACTTGTGAGG TGTGGAATGA AGCCGGCAAC
    GCTCGAACTC AAGCTTCACT CACTGTACAA GAGCCTCAAG ATGGTGTTCA GCCCTGGTTC
    ATTACTAAAA CCAAGTCCTC CACCGCCTCT CTGGGTCAAC ATGCTCTGCT CTCTTGCGCC
    ATTGCTGGTG ACCCCTTCCC AGAATTCCTC TGGATGAAGG ATGGACAGGT AATATCTGGT
    GATGGGGACT TTGAGATCCT ACAGAAGGAA GATGTTGTCT CGCTGCTTAT CAGGAAAGTG
    GCGATGCATC ACGCTGGAAA CTACGAGATT CATCTTAAAA ATAAAGTAGG AGAGTGCCGG
    TCAGTGGCCA CTCTGCATGT CAGCGAGGAT CCCATGCTGT CTGCTGATGA TGCTAACAAA
    CGCCGAGGGG TGGAGAAGAC TGCGCTAGGC AGGGGAGGAC AGCCAAAGAC TCCCAGCCCT
    GCAGCATCGT TGCACTCTGA GGAACATAGC AGTGGGACAG ATCAGCAAGA AGAGGAGGGG
    GGCTCCACGC GTGGGCTTCT CAAACGGCGT GTAGAAACGA AGGAGCATCG GGAAGATCAG
    ATCCGACAGC AGGAGGCCGA ACAGATCGAC TTCAGAACCG TGCTCGGCCG CAAGGTCACC
    ACTAAGAGCG TCTCAGAGGA AGATCTGAAG GAGATCACGG CTGAGCAGAT GGACTTCAGA
    GGGAACCTCC AACGGCAGAT CAAGCCCAAG ACTCAGACAG AAGAGCAACG CAAAGTCAAC
    ACTCCGCAGC AGGTGGACTT CAGAGCGGTG CTAGGGAAAA AAGCAAGCCA AGGCCCAAAA
    CCTGCTCCTG GATCCTTGAA AACGCAACCT AGCAAAAACG AAGCCGTTGA CTTCAGATCT
    GTTTTAGGCA ATAAGAAAAA GCCACCAACC CAGGATAGGA ATGGAGAAAG CCAAGGCGAC
    CCCAGAGGAA AGGAAAATGA CGTGAATTGT GTGGATGGAG AGATAAAGGA GACCACGAAG
    GCAGCTGGGA AGAAGGAGCC TGTTTTTGTA GAGAAACTGA GTGATGTGAC TGTGCTGGAC
    GGAGAGCGTT TGCATCTACA GTGCCAGCTG TCATCTGAGT CTCCTGCTAA AGTGACATGG
    ATGCTTGATG GAAAAGTCAT CAGACCCTCC AAATTCATCA TTATCACTAA TGAGGGTGAT
    CGGTACTCTT TATCTATTGA TAAAGCTCTG CCTGAGGATG AAGGGCAGTA CGTGTGCAGG
    GCAGAGACCG ATGAGGGCAA GACTGAGTGT TCCTGTGTGG TCCTAGTGAA CGATCCCAGC
    GTCTCCACAC CAGATGATAA GCGGAGCAGG AAGCCTTCTA CCCCGACCAC AGAGAATGAG
    GCCAAAATAA AGAAACCCCC ACCCAAAACA CTGCCTAAAC AAGGTGCTCC GCCTCAAATC
    CAGCAGTTTC CTGATGATAT GAAGATCATG GCAGGTCAGG AGGTGATTCT GCAGTGTAAG
    TTCACTGGAG CACTCCCCAT CAGCTGCACC TGGCTCAAGT TCCGCAAACC AATTCAGGAA
    GGTCAGGGCA TCTCAATAGT CAGCACAGAC AGTAGCAGTC AGCTGACCAT CACAGAAGGA
    CAGCAGGAGC ACTGTGGCTG CTATACCATA GAGATCAAGA ACAGCTTTGG TGTGAGACAG
    GCTGCCCTCA ACCTCACTAT AGTCGATAAG CCTGACCCCC CTGCCCGTGT CCCAGCAGCC
    TCAGATATCC GTAAGTCCAG CCTCACGCTG TCCTGGTATG GGCCAACATA CGACGGTGGC
    AGCGCTGTCC AATCCTACAA CCTGGAGGCT TGGAACTCTC TGGACATGAA GTGGACGGAC
    GTGGCATCCT GTAACAGCAC ATCTTATAAC GTGCAGGACC TTCTTCCAGA CAGACAGTAC
    AAGTTTCGAG TGAGAGCTGT AAATATTTAT GGCGTCGGGG AGCCCAGTGC CGAGTCTGAG
    CCCGTGCAGG TGGGGCTGGA GGCAGAGGGG GAAAATAAGG AGGATGAAGT CGAGCCCTCG
    GATGATGAGT CAGAGAAAGT ACCAGACTAC AGAAATGTGA CGATCAAAAC AGACGTAAAA
    GTAAAAGATT TTTATGATGT AGAAGACCGA CTGGGCACAG GGAAATTTGG GACCGTTTTC
    AAACTGATCG AGAAGTCCAC TAAAAAGGTT TGGGCTGGGA AATTCATAAA AGCATATTCT
    GCCAAAGAAA AAGAAAACGT CAGGCAGGAA ATTGCAATCA TGAATGACCT GCATCATCCA
    AAGCTGGTGC AGTGCATCGA TGCTTTTGAG GGAAAGACCG ACATCGTAAT GGTGCTGGAA
    ATGATCTCTG GAGGGGAACT GTTTGACCGG ATCATAGATG AGGACTTTGA GCTGACGGAG
    AGAGAGGTGA TCAAATATAT GCTTCAGATT GTGGACGGGG TCAATTTCAT CCATAAGAAA
    GGCATCGTCC ACCTGGACCT CAAACCTGAG AACATCATGT GTGTTAATAA GACGGGCAGC
    AAAATCAAAC TCATCGACTT TGGACTGGCA CGCAGACTTG AGAATGCTGG ATCTCTCAAA
    GTCCTGTTTG GAACCCCTGA GTTTGTGGCT CCAGAGGTGA TCAATTATGA GGCCATCAGT
    TATGCGACAG ACATGTGGAG TATAGGAGTG ATCTGCTACA TCCTTGTCAG TGGTTTGTCT
    CCGTTCATGG GGGACAATGA TAACGAGACG CTGTCAAATG TGACCTCAGC GACGTGGGAC
    TTTGAAGACG AGGCATTTGA TGAGATCTCA GAAGAGGCCA AAGACTTTAT CAGTAATCTG
    CTAAAGAAAG ACATGAAAGC TCGCCTGACC TGTGACCAGT GTTTCCAGCA TCCATGGCTT
    AAACAAGACA CCACCAACAT GGAGGCCAAG AAACTTTCCA AAGAGAGGAT GAAGAAGTAT
    ATTCTGAGAA GAAAGTGGCA GAAAACAGGG CATGCGGTAC GAGCTATTGG TCGACTGTCT
    AACATGGCAT TGATGGCAGG TGTTCATGCC AAAAAAAGTT CCCCCACAGA AGCAGATGCT
    AAACAGTTTG CTGCTGAATT GGAGGCAAAA ACTTTGATGA AGCCCACGTT CAGCTCTGTT
    ATCAAAGATG TGGAGGTGGT GGAGGGAAGC GCTGCTCGAT TTGACTGCAA AATTGAAGGT
    TATCCTGACC CAGAGGTGGT GTGGTATAAG GACGATCAGC CCATTAAAGA GACGCGCCAT
    TTTCAGATAG ATTATGACGA GGACGGAAAC TGTAGTCTGG TCATTTCTGA GGTCAACGGC
    GATGATGACG CAAAATATAC CTGCAAGGCC ATGAACAGCC TCGGGGAGAC CACCTGCACC
    GCTGAACTCA TCGTAGAGGT GATGGGAGAA GAAGAAGAGG AGGAAGAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213228.1
    CDS36..5606
    Translation

    Target ORF information:

    RefSeq Version XM_017357739.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio myosin, light chain kinase a (mylka), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017357739.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGCAGCT CTCAGCCTGT GATGTTAGGA TTCATGCCAG CAAAGTTTGG TGCCGACAGG 
    GAAAACACGT TAAACGCAGA GTGGAGTCGG GACAGCGGGG TCTGGACGCG CTCTCGACAC
    TGCACTCCGG TGAATGACAC TAATCTGCAC TGCTGTCTAC ATGGAATGAT GGCCGATGTT
    CAGTTTGTTA CAGGATCTCA TGTCTCCAAG ACAGCTCTGA CTCTGGGACG AGGTTCCCCC
    AGCAAAGAGC CCCCCGTTTT TACCCTCCCG CCCCGCAATA CCCGTGTCTG CCAAGGGGGA
    ACTGCGCGCC TTGAGGGGAA GGTACGTGGT TTCCCAGAGC CTCAGGTATG CTGGTTTAGG
    AATGGCAAGC TACTGATAGC AGGAGATCAT TATTCTATGG AACAGAGTGT CCGTGGAACA
    TTTAGTCTGG TGGTCCAGGA GGTAGAGGAT GCCGATGGAG GACGTTACAC CTGTGAAGCT
    GTTAACGATG CTGGGAGCTG CCAGGTTACT GTGGAGATCA TTGTGGAAGG AAACACAGGA
    AAGAAATACA GTCTTCCTTC CACAAACAGA ACAGGATCTA TGGGAGCTCC CGCAGTAGAA
    AACAGAGCCA GTATCTGGGG TGAAAGCCCC CCAAAGTTTG TGGCTAAACC TTCTCGCCTG
    TATCTCAAAG TGGGACAAAC TGGCAAATTT TCGGCCAAGA TCACAGGACG ACCCCAGCCA
    CTGGTGCAGT GGTACAAGGG AGAAGAGATG ATCCAGCAAT CAGATCACTA TAACATGTTT
    GAGAGGTCAG GAATGCATTT CCTGGAGATC CGCAATGTGT TCTCTGAGGA TGCTGGCATG
    TACACCTGTC TGGTGGCCAA CAGTGCTGGA AAAGCCTCTG CAGCCGCTGA ACTCATTATG
    CAAGATGGAG ACACCGAGAC AGTCATCATG GCCAAACCAT CTCACAATCC CTCCAAGTCA
    TTCATGCTCA AAGACACTTC CAACACTCTT GCCAACGAAC AGAAAACTTC CAGCACATCC
    ATCGAAACCA TAATCACAGA AACACCCACA CCGCTGTCCA CTACTACTGT TCCCACTGTC
    CCATCCATGA ACAGAGGAGA AACAACAATA GTAACATCAT CACTTCACTC ACAACCTATC
    AGCAGGTCCA CAGATGGAAG ATCAGCAACT GGCCAAAATA CTTCAAACTT AGTCAATCTA
    AGAAGTCAAA ACAGTGTTAA GAATGAGACG CATGGCACAA AGAGAGTTTT GGGTATGTTC
    AGAGCTCCGG GACAGATGCC ACAATCATCA CAGATAGAAT CCAGCACTCG CCAACAAACC
    AAAGAAACCA GAATGGATGT GCTAGAAACT CCACCCAGTT TTGACTCGCA GCCCCAGAGC
    CAAGAGGTCC TGCAGGGTGA AAAGGTCACT TTCAAATGCC AAGTGTCCGG GTCTCCAGTG
    CCTGCAGTGA GCTGGCTGAA AGATGGAGCT CCTCTCAGAC AGAGATCAGA TGTCAGCACT
    CGACAGGAGG CCGGCCTGCA TGTTCTGTGT CTGGACAATG CTCACATAAC TGACACGGGA
    CAGTATGGCT GCACTGCTGC CAACAGAAGA GGAAACACCG CGGCCCACTG GAGTCTGACC
    GTCAAGCGTG TGCGAGTGGA TGCGAAGCCC CCAGTGTTCA GCTCTGCTCT GAAGGATTGT
    TCGGTGTGTG AAGGACAGGA ATTTACCCTG CAGTGTTCAG TGGAGGGCAA ACCACAGCCT
    GAGCTCTCCT GGCTGCACAA CGATAAGCCC ATTCCATTCT CCCATACCAT GTTTGACAAC
    AGTAAAGCTC AGCTGAGAGT TCAGGCGGCT GTATTGGAGG ATGCAGGCGT TTATTCGTGT
    GTAGCAGTGA ATGAGCATGG CAGAGCGGTG TGTACAGCCC ATGTGCTGGT TAAAGCAAGG
    CCAAGGGTGA AAAGGTCTGC TGCTCCTCCT GTTACTGATC TGAGGAGAAC ATCTGCTCCG
    CTGTTCCTCA AAGGTCTACA GGACCTCAGG GTGATGGACG GCAGTCAGGT CATCATGACC
    GTGGAAGTGA CAGGAAACCC TGCACCTGAG GTGGTCTGGC TGCATAATGG AAAAGAGATC
    CAGGAGTCAG AGGACTTTCA CTTTGAAAAG AAGGGCGGTC AGTATACACT CTTCATACAG
    GAGGTGTTTC CTGAGGACAC GGGGACATAC ACTTGTGAGG TGTGGAATGA AGCCGGCAAC
    GCTCGAACTC AAGCTTCACT CACTGTACAA GAGCCTCAAG ATGGTGTTCA GCCCTGGTTC
    ATTACTAAAA CCAAGTCCTC CACCGCCTCT CTGGGTCAAC ATGCTCTGCT CTCTTGCGCC
    ATTGCTGGTG ACCCCTTCCC AGAATTCCTC TGGATGAAGG ATGGACAGGT AATATCTGGT
    GATGGGGACT TTGAGATCCT ACAGAAGGAA GATGTTGTCT CGCTGCTTAT CAGGAAAGTG
    GCGATGCATC ACGCTGGAAA CTACGAGATT CATCTTAAAA ATAAAGTAGG AGAGTGCCGG
    TCAGTGGCCA CTCTGCATGT CAGCGAGGAT CCCATGCTGT CTGCTGATGA TGCTAACAAA
    CGCCGAGGGG TGGAGAAGAC TGCGCTAGGC AGGGGAGGAC AGCCAAAGAC TCCCAGCCCT
    GCAGCATCGT TGCACTCTGA GGAACATAGC AGTGGGACAG ATCAGCAAGA AGAGGAGGGG
    GGCTCCACGC GTGGGCTTCT CAAACGGCGT GTAGAAACGA AGGAGCATCG GGAAGATCAG
    ATCCGACAGC AGGAGGCCGA ACAGATCGAC TTCAGAACCG TGCTCGGCCG CAAGGTCACC
    ACTAAGAGCG TCTCAGAGGA AGATCTGAAG GAGATCACGG CTGAGCAGAT GGACTTCAGA
    GGGAACCTCC AACGGCAGAT CAAGCCCAAG ACTCAGACAG AAGAGCAACG CAAAGTCAAC
    ACTCCGCAGC AGGTGGACTT CAGAGCGGTG CTAGGGAAAA AAGCAAGCCA AGGCCCAAAA
    CCTGCTCCTG GATCCTTGAA AACGCAACCT AGCAAAAACG AAGCCGTTGA CTTCAGATCT
    GTTTTAGGCA ATAAGAAAAA GCCACCAACC CAGGATAGGA ATGGAGAAAG CCAAGGCGAC
    CCCAGAGGAA AGGAAAATGA CGTGAATTGT GTGGATGGAG AGATAAAGGA GACCACGAAG
    GCAGCTGGGA AGAAGGAGCC TGTTTTTGTA GAGAAACTGA GTGATGTGAC TGTGCTGGAC
    GGAGAGCGTT TGCATCTACA GTGCCAGCTG TCATCTGAGT CTCCTGCTAA AGTGACATGG
    ATGCTTGATG GAAAAGTCAT CAGACCCTCC AAATTCATCA TTATCACTAA TGAGGGTGAT
    CGGTACTCTT TATCTATTGA TAAAGCTCTG CCTGAGGATG AAGGGCAGTA CGTGTGCAGG
    GCAGAGACCG ATGAGGGCAA GACTGAGTGT TCCTGTGTGG TCCTAGTGAA CGATCCCAGC
    GTCTCCACAC CAGATGATAA GCGGAGCAGG AAGCCTTCTA CCCCGACCAC AGAGAATGAG
    GCCAAAATAA AGAAACCCCC ACCCAAAACA CTGCCTAAAC AAGGTGCTCC GCCTCAAATC
    CAGCAGTTTC CTGATGATAT GAAGATCATG GCAGGTCAGG AGGTGATTCT GCAGTGTAAG
    TTCACTGGAG CACTCCCCAT CAGCTGCACC TGGCTCAAGT TCCGCAAACC AATTCAGGAA
    GGTCAGGGCA TCTCAATAGT CAGCACAGAC AGTAGCAGTC AGCTGACCAT CACAGAAGGA
    CAGCAGGAGC ACTGTGGCTG CTATACCATA GAGATCAAGA ACAGCTTTGG TGTGAGACAG
    GCTGCCCTCA ACCTCACTAT AGTCGATAAG CCTGACCCCC CTGCCCGTGT CCCAGCAGCC
    TCAGATATCC GTAAGTCCAG CCTCACGCTG TCCTGGTATG GGCCAACATA CGACGGTGGC
    AGCGCTGTCC AATCCTACAA CCTGGAGGCT TGGAACTCTC TGGACATGAA GTGGACGGAC
    GTGGCATCCT GTAACAGCAC ATCTTATAAC GTGCAGGACC TTCTTCCAGA CAGACAGTAC
    AAGTTTCGAG TGAGAGCTGT AAATATTTAT GGCGTCGGGG AGCCCAGTGC CGAGTCTGAG
    CCCGTGCAGG TGGGGCTGGA GGCAGAGGGG GAAAATAAGG AGGATGAAGT CGAGCCCTCG
    GATGATGAGT CAGAGAAAGT ACCAGACTAC AGAAATGTGA CGATCAAAAC AGACGTAAAA
    GTAAAAGATT TTTATGATGT AGAAGACCGA CTGGGCACAG GGAAATTTGG GACCGTTTTC
    AAACTGATCG AGAAGTCCAC TAAAAAGGTT TGGGCTGGGA AATTCATAAA AGCATATTCT
    GCCAAAGAAA AAGAAAACGT CAGGCAGGAA ATTGCAATCA TGAATGACCT GCATCATCCA
    AAGCTGGTGC AGTGCATCGA TGCTTTTGAG GGAAAGACCG ACATCGTAAT GGTGCTGGAA
    ATGATCTCTG GAGGGGAACT GTTTGACCGG ATCATAGATG AGGACTTTGA GCTGACGGAG
    AGAGAGGTGA TCAAATATAT GCTTCAGATT GTGGACGGGG TCAATTTCAT CCATAAGAAA
    GGCATCGTCC ACCTGGACCT CAAACCTGAG AACATCATGT GTGTTAATAA GACGGGCAGC
    AAAATCAAAC TCATCGACTT TGGACTGGCA CGCAGACTTG AGAATGCTGG ATCTCTCAAA
    GTCCTGTTTG GAACCCCTGA GTTTGTGGCT CCAGAGGTGA TCAATTATGA GGCCATCAGT
    TATGCGACAG ACATGTGGAG TATAGGAGTG ATCTGCTACA TCCTTGTCAG TGGTTTGTCT
    CCGTTCATGG GGGACAATGA TAACGAGACG CTGTCAAATG TGACCTCAGC GACGTGGGAC
    TTTGAAGACG AGGCATTTGA TGAGATCTCA GAAGAGGCCA AAGACTTTAT CAGTAATCTG
    CTAAAGAAAG ACATGAAAGC TCGCCTGACC TGTGACCAGT GTTTCCAGCA TCCATGGCTT
    AAACAAGACA CCACCAACAT GGAGGCCAAG AAACTTTCCA AAGAGAGGAT GAAGAAGTAT
    ATTCTGAGAA GAAAGTGGCA GAAAACAGGG CATGCGGTAC GAGCTATTGG TCGACTGTCT
    AACATGGCAT TGATGGCAGG TGTTCATGCC AAAAAAAGTT CCCCCACAGA AGCAGATGCT
    AAACAGTTTG CTGCTGAATT GGAGGCAAAA ACTTTGATGA AGCCCACGTT CAGCTCTGTT
    ATCAAAGATG TGGAGGTGGT GGAGGGAAGC GCTGCTCGAT TTGACTGCAA AATTGAAGGT
    TATCCTGACC CAGAGGTGGT GTGGTATAAG GACGATCAGC CCATTAAAGA GACGCGCCAT
    TTTCAGATAG ATTATGACGA GGACGGAAAC TGTAGTCTGG TCATTTCTGA GGTCAACGGC
    GATGATGACG CAAAATATAC CTGCAAGGCC ATGAACAGCC TCGGGGAGAC CACCTGCACC
    GCTGAACTCA TCGTAGAGGT GATGGGAGAA GAAGAAGAGG AGGAAGAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa60286
    Clone ID Related Accession (Same CDS sequence) XM_017357740.1
    Accession Version XM_017357740.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5178bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product myosin light chain kinase, smooth muscle isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGAACAGA GTGTCCGTGG AACATTTAGT CTGGTGGTCC AGGAGGTAGA GGATGCCGAT 
    GGAGGACGTT ACACCTGTGA AGCTGTTAAC GATGCTGGGA GCTGCCAGGT TACTGTGGAG
    ATCATTGTGG AAGGAAACAC AGGAAAGAAA TACAGTCTTC CTTCCACAAA CAGAACAGGC
    AGATCTATGG GAGCTCCCGC AGTAGAAAAC AGAGCCAGTA TCTGGGGTGA AAGCCCCCCA
    AAGTTTGTGG CTAAACCTTC TCGCCTGTAT CTCAAAGTGG GACAAACTGG CAAATTTTCG
    GCCAAGATCA CAGGACGACC CCAGCCACTG GTGCAGTGGT ACAAGGGAGA AGAGATGATC
    CAGCAATCAG ATCACTATAA CATGTTTGAG AGGTCAGGAA TGCATTTCCT GGAGATCCGC
    AATGTGTTCT CTGAGGATGC TGGCATGTAC ACCTGTCTGG TGGCCAACAG TGCTGGAAAA
    GCCTCTGCAG CCGCTGAACT CATTATGCAA GATGGAGACA CCGAGACAGT CATCATGGCC
    AAACCATCTC ACAATCCCTC CAAGTCATTC ATGCTCAAAG ACACTTCCAA CACTCTTGCC
    AACGAACAGA AAACTTCCAG CACATCCATC GAAACCATAA TCACAGAAAC ACCCACACCG
    CTGTCCACTA CTACTGTTCC CACTGTCCCA TCCATGAACA GAGGAGAAAC AACAATAGTA
    ACATCATCAC TTCACTCACA ACCTATCAGC AGGTCCACAG ATGGAAGATC AGCAACTGGC
    CAAAATACTT CAAACTTAGT CAATCTAAGA AGTCAAAACA GTGTTAAGAA TGAGACGCAT
    GGCACAAAGA GAGTTTTGGG TATGTTCAGA GCTCCGGGAC AGATGCCACA ATCATCACAG
    ATAGAATCCA GCACTCGCCA ACAAACCAAA GAAACCAGAA TGGATGTGCT AGAAACTCCA
    CCCAGTTTTG ACTCGCAGCC CCAGAGCCAA GAGGTCCTGC AGGGTGAAAA GGTCACTTTC
    AAATGCCAAG TGTCCGGGTC TCCAGTGCCT GCAGTGAGCT GGCTGAAAGA TGGAGCTCCT
    CTCAGACAGA GATCAGATGT CAGCACTCGA CAGGAGGCCG GCCTGCATGT TCTGTGTCTG
    GACAATGCTC ACATAACTGA CACGGGACAG TATGGCTGCA CTGCTGCCAA CAGAAGAGGA
    AACACCGCGG CCCACTGGAG TCTGACCGTC AAGCGTGTGC GAGTGGATGC GAAGCCCCCA
    GTGTTCAGCT CTGCTCTGAA GGATTGTTCG GTGTGTGAAG GACAGGAATT TACCCTGCAG
    TGTTCAGTGG AGGGCAAACC ACAGCCTGAG CTCTCCTGGC TGCACAACGA TAAGCCCATT
    CCATTCTCCC ATACCATGTT TGACAACAGT AAAGCTCAGC TGAGAGTTCA GGCGGCTGTA
    TTGGAGGATG CAGGCGTTTA TTCGTGTGTA GCAGTGAATG AGCATGGCAG AGCGGTGTGT
    ACAGCCCATG TGCTGGTTAA AGCAAGGCCA AGGGTGAAAA GGTCTGCTGC TCCTCCTGTT
    ACTGATCTGA GGAGAACATC TGCTCCGCTG TTCCTCAAAG GTCTACAGGA CCTCAGGGTG
    ATGGACGGCA GTCAGGTCAT CATGACCGTG GAAGTGACAG GAAACCCTGC ACCTGAGGTG
    GTCTGGCTGC ATAATGGAAA AGAGATCCAG GAGTCAGAGG ACTTTCACTT TGAAAAGAAG
    GGCGGTCAGT ATACACTCTT CATACAGGAG GTGTTTCCTG AGGACACGGG GACATACACT
    TGTGAGGTGT GGAATGAAGC CGGCAACGCT CGAACTCAAG CTTCACTCAC TGTACAAGAG
    CCTCAAGATG GTGTTCAGCC CTGGTTCATT ACTAAAACCA AGTCCTCCAC CGCCTCTCTG
    GGTCAACATG CTCTGCTCTC TTGCGCCATT GCTGGTGACC CCTTCCCAGA ATTCCTCTGG
    ATGAAGGATG GACAGGTAAT ATCTGGTGAT GGGGACTTTG AGATCCTACA GAAGGAAGAT
    GTTGTCTCGC TGCTTATCAG GAAAGTGGCG ATGCATCACG CTGGAAACTA CGAGATTCAT
    CTTAAAAATA AAGTAGGAGA GTGCCGGTCA GTGGCCACTC TGCATGTCAG CGAGGATCCC
    ATGCTGTCTG CTGATGATGC TAACAAACGC CGAGGGGTGG AGAAGACTGC GCTAGGCAGG
    GGAGGACAGC CAAAGACTCC CAGCCCTGCA GCATCGTTGC ACTCTGAGGA ACATAGCAGT
    GGGACAGATC AGCAAGAAGA GGAGGGGGGC TCCACGCGTG GGCTTCTCAA ACGGCGTGTA
    GAAACGAAGG AGCATCGGGA AGATCAGATC CGACAGCAGG AGGCCGAACA GATCGACTTC
    AGAACCGTGC TCGGCCGCAA GGTCACCACT AAGAGCGTCT CAGAGGAAGA TCTGAAGGAG
    ATCACGGCTG AGCAGATGGA CTTCAGAGGG AACCTCCAAC GGCAGATCAA GCCCAAGACT
    CAGACAGAAG AGCAACGCAA AGTCAACACT CCGCAGCAGG TGGACTTCAG AGCGGTGCTA
    GGGAAAAAAG CAAGCCAAGG CCCAAAACCT GCTCCTGGAT CCTTGAAAAC GCAACCTAGC
    AAAAACGAAG CCGTTGACTT CAGATCTGTT TTAGGCAATA AGAAAAAGCC ACCAACCCAG
    GATAGGAATG GAGAAAGCCA AGGCGACCCC AGAGGAAAGG AAAATGACGT GAATTGTGTG
    GATGGAGAGA TAAAGGAGAC CACGAAGGCA GCTGGGAAGA AGGAGCCTGT TTTTGTAGAG
    AAACTGAGTG ATGTGACTGT GCTGGACGGA GAGCGTTTGC ATCTACAGTG CCAGCTGTCA
    TCTGAGTCTC CTGCTAAAGT GACATGGATG CTTGATGGAA AAGTCATCAG ACCCTCCAAA
    TTCATCATTA TCACTAATGA GGGTGATCGG TACTCTTTAT CTATTGATAA AGCTCTGCCT
    GAGGATGAAG GGCAGTACGT GTGCAGGGCA GAGACCGATG AGGGCAAGAC TGAGTGTTCC
    TGTGTGGTCC TAGTGAACGA TCCCAGCGTC TCCACACCAG ATGATAAGCG GAGCAGGAAG
    CCTTCTACCC CGACCACAGA GAATGAGGCC AAAATAAAGA AACCCCCACC CAAAACACTG
    CCTAAACAAG GTGCTCCGCC TCAAATCCAG CAGTTTCCTG ATGATATGAA GATCATGGCA
    GGTCAGGAGG TGATTCTGCA GTGTAAGTTC ACTGGAGCAC TCCCCATCAG CTGCACCTGG
    CTCAAGTTCC GCAAACCAAT TCAGGAAGGT CAGGGCATCT CAATAGTCAG CACAGACAGT
    AGCAGTCAGC TGACCATCAC AGAAGGACAG CAGGAGCACT GTGGCTGCTA TACCATAGAG
    ATCAAGAACA GCTTTGGTGT GAGACAGGCT GCCCTCAACC TCACTATAGT CGATAAGCCT
    GACCCCCCTG CCCGTGTCCC AGCAGCCTCA GATATCCGTA AGTCCAGCCT CACGCTGTCC
    TGGTATGGGC CAACATACGA CGGTGGCAGC GCTGTCCAAT CCTACAACCT GGAGGCTTGG
    AACTCTCTGG ACATGAAGTG GACGGACGTG GCATCCTGTA ACAGCACATC TTATAACGTG
    CAGGACCTTC TTCCAGACAG ACAGTACAAG TTTCGAGTGA GAGCTGTAAA TATTTATGGC
    GTCGGGGAGC CCAGTGCCGA GTCTGAGCCC GTGCAGGTGG GGCTGGAGGC AGAGGGGGAA
    AATAAGGAGG ATGAAGTCGA GCCCTCGGAT GATGAGTCAG AGAAAGTACC AGACTACAGA
    AATGTGACGA TCAAAACAGA CGTAAAAGTA AAAGATTTTT ATGATGTAGA AGACCGACTG
    GGCACAGGGA AATTTGGGAC CGTTTTCAAA CTGATCGAGA AGTCCACTAA AAAGGTTTGG
    GCTGGGAAAT TCATAAAAGC ATATTCTGCC AAAGAAAAAG AAAACGTCAG GCAGGAAATT
    GCAATCATGA ATGACCTGCA TCATCCAAAG CTGGTGCAGT GCATCGATGC TTTTGAGGGA
    AAGACCGACA TCGTAATGGT GCTGGAAATG ATCTCTGGAG GGGAACTGTT TGACCGGATC
    ATAGATGAGG ACTTTGAGCT GACGGAGAGA GAGGTGATCA AATATATGCT TCAGATTGTG
    GACGGGGTCA ATTTCATCCA TAAGAAAGGC ATCGTCCACC TGGACCTCAA ACCTGAGAAC
    ATCATGTGTG TTAATAAGAC GGGCAGCAAA ATCAAACTCA TCGACTTTGG ACTGGCACGC
    AGACTTGAGA ATGCTGGATC TCTCAAAGTC CTGTTTGGAA CCCCTGAGTT TGTGGCTCCA
    GAGGTGATCA ATTATGAGGC CATCAGTTAT GCGACAGACA TGTGGAGTAT AGGAGTGATC
    TGCTACATCC TTGTCAGTGG TTTGTCTCCG TTCATGGGGG ACAATGATAA CGAGACGCTG
    TCAAATGTGA CCTCAGCGAC GTGGGACTTT GAAGACGAGG CATTTGATGA GATCTCAGAA
    GAGGCCAAAG ACTTTATCAG TAATCTGCTA AAGAAAGACA TGAAAGCTCG CCTGACCTGT
    GACCAGTGTT TCCAGCATCC ATGGCTTAAA CAAGACACCA CCAACATGGA GGCCAAGAAA
    CTTTCCAAAG AGAGGATGAA GAAGTATATT CTGAGAAGAA AGTGGCAGAA AACAGGGCAT
    GCGGTACGAG CTATTGGTCG ACTGTCTAAC ATGGCATTGA TGGCAGGTGT TCATGCCAAA
    AAAAGTTCCC CCACAGAAGC AGATGCTAAA CAGTTTGCTG CTGAATTGGA GGCAAAAACT
    TTGATGAAGC CCACGTTCAG CTCTGTTATC AAAGATGTGG AGGTGGTGGA GGGAAGCGCT
    GCTCGATTTG ACTGCAAAAT TGAAGGTTAT CCTGACCCAG AGGTGGTGTG GTATAAGGAC
    GATCAGCCCA TTAAAGAGAC GCGCCATTTT CAGATAGATT ATGACGAGGA CGGAAACTGT
    AGTCTGGTCA TTTCTGAGGT CAACGGCGAT GATGACGCAA AATATACCTG CAAGGCCATG
    AACAGCCTCG GGGAGACCAC CTGCACCGCT GAACTCATCG TAGAGGTGAT GGGAGAAGAA
    GAAGAGGAGG AAGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213229.1
    CDS263..5440
    Translation

    Target ORF information:

    RefSeq Version XM_017357740.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio myosin, light chain kinase a (mylka), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017357740.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGGAACAGA GTGTCCGTGG AACATTTAGT CTGGTGGTCC AGGAGGTAGA GGATGCCGAT 
    GGAGGACGTT ACACCTGTGA AGCTGTTAAC GATGCTGGGA GCTGCCAGGT TACTGTGGAG
    ATCATTGTGG AAGGAAACAC AGGAAAGAAA TACAGTCTTC CTTCCACAAA CAGAACAGGC
    AGATCTATGG GAGCTCCCGC AGTAGAAAAC AGAGCCAGTA TCTGGGGTGA AAGCCCCCCA
    AAGTTTGTGG CTAAACCTTC TCGCCTGTAT CTCAAAGTGG GACAAACTGG CAAATTTTCG
    GCCAAGATCA CAGGACGACC CCAGCCACTG GTGCAGTGGT ACAAGGGAGA AGAGATGATC
    CAGCAATCAG ATCACTATAA CATGTTTGAG AGGTCAGGAA TGCATTTCCT GGAGATCCGC
    AATGTGTTCT CTGAGGATGC TGGCATGTAC ACCTGTCTGG TGGCCAACAG TGCTGGAAAA
    GCCTCTGCAG CCGCTGAACT CATTATGCAA GATGGAGACA CCGAGACAGT CATCATGGCC
    AAACCATCTC ACAATCCCTC CAAGTCATTC ATGCTCAAAG ACACTTCCAA CACTCTTGCC
    AACGAACAGA AAACTTCCAG CACATCCATC GAAACCATAA TCACAGAAAC ACCCACACCG
    CTGTCCACTA CTACTGTTCC CACTGTCCCA TCCATGAACA GAGGAGAAAC AACAATAGTA
    ACATCATCAC TTCACTCACA ACCTATCAGC AGGTCCACAG ATGGAAGATC AGCAACTGGC
    CAAAATACTT CAAACTTAGT CAATCTAAGA AGTCAAAACA GTGTTAAGAA TGAGACGCAT
    GGCACAAAGA GAGTTTTGGG TATGTTCAGA GCTCCGGGAC AGATGCCACA ATCATCACAG
    ATAGAATCCA GCACTCGCCA ACAAACCAAA GAAACCAGAA TGGATGTGCT AGAAACTCCA
    CCCAGTTTTG ACTCGCAGCC CCAGAGCCAA GAGGTCCTGC AGGGTGAAAA GGTCACTTTC
    AAATGCCAAG TGTCCGGGTC TCCAGTGCCT GCAGTGAGCT GGCTGAAAGA TGGAGCTCCT
    CTCAGACAGA GATCAGATGT CAGCACTCGA CAGGAGGCCG GCCTGCATGT TCTGTGTCTG
    GACAATGCTC ACATAACTGA CACGGGACAG TATGGCTGCA CTGCTGCCAA CAGAAGAGGA
    AACACCGCGG CCCACTGGAG TCTGACCGTC AAGCGTGTGC GAGTGGATGC GAAGCCCCCA
    GTGTTCAGCT CTGCTCTGAA GGATTGTTCG GTGTGTGAAG GACAGGAATT TACCCTGCAG
    TGTTCAGTGG AGGGCAAACC ACAGCCTGAG CTCTCCTGGC TGCACAACGA TAAGCCCATT
    CCATTCTCCC ATACCATGTT TGACAACAGT AAAGCTCAGC TGAGAGTTCA GGCGGCTGTA
    TTGGAGGATG CAGGCGTTTA TTCGTGTGTA GCAGTGAATG AGCATGGCAG AGCGGTGTGT
    ACAGCCCATG TGCTGGTTAA AGCAAGGCCA AGGGTGAAAA GGTCTGCTGC TCCTCCTGTT
    ACTGATCTGA GGAGAACATC TGCTCCGCTG TTCCTCAAAG GTCTACAGGA CCTCAGGGTG
    ATGGACGGCA GTCAGGTCAT CATGACCGTG GAAGTGACAG GAAACCCTGC ACCTGAGGTG
    GTCTGGCTGC ATAATGGAAA AGAGATCCAG GAGTCAGAGG ACTTTCACTT TGAAAAGAAG
    GGCGGTCAGT ATACACTCTT CATACAGGAG GTGTTTCCTG AGGACACGGG GACATACACT
    TGTGAGGTGT GGAATGAAGC CGGCAACGCT CGAACTCAAG CTTCACTCAC TGTACAAGAG
    CCTCAAGATG GTGTTCAGCC CTGGTTCATT ACTAAAACCA AGTCCTCCAC CGCCTCTCTG
    GGTCAACATG CTCTGCTCTC TTGCGCCATT GCTGGTGACC CCTTCCCAGA ATTCCTCTGG
    ATGAAGGATG GACAGGTAAT ATCTGGTGAT GGGGACTTTG AGATCCTACA GAAGGAAGAT
    GTTGTCTCGC TGCTTATCAG GAAAGTGGCG ATGCATCACG CTGGAAACTA CGAGATTCAT
    CTTAAAAATA AAGTAGGAGA GTGCCGGTCA GTGGCCACTC TGCATGTCAG CGAGGATCCC
    ATGCTGTCTG CTGATGATGC TAACAAACGC CGAGGGGTGG AGAAGACTGC GCTAGGCAGG
    GGAGGACAGC CAAAGACTCC CAGCCCTGCA GCATCGTTGC ACTCTGAGGA ACATAGCAGT
    GGGACAGATC AGCAAGAAGA GGAGGGGGGC TCCACGCGTG GGCTTCTCAA ACGGCGTGTA
    GAAACGAAGG AGCATCGGGA AGATCAGATC CGACAGCAGG AGGCCGAACA GATCGACTTC
    AGAACCGTGC TCGGCCGCAA GGTCACCACT AAGAGCGTCT CAGAGGAAGA TCTGAAGGAG
    ATCACGGCTG AGCAGATGGA CTTCAGAGGG AACCTCCAAC GGCAGATCAA GCCCAAGACT
    CAGACAGAAG AGCAACGCAA AGTCAACACT CCGCAGCAGG TGGACTTCAG AGCGGTGCTA
    GGGAAAAAAG CAAGCCAAGG CCCAAAACCT GCTCCTGGAT CCTTGAAAAC GCAACCTAGC
    AAAAACGAAG CCGTTGACTT CAGATCTGTT TTAGGCAATA AGAAAAAGCC ACCAACCCAG
    GATAGGAATG GAGAAAGCCA AGGCGACCCC AGAGGAAAGG AAAATGACGT GAATTGTGTG
    GATGGAGAGA TAAAGGAGAC CACGAAGGCA GCTGGGAAGA AGGAGCCTGT TTTTGTAGAG
    AAACTGAGTG ATGTGACTGT GCTGGACGGA GAGCGTTTGC ATCTACAGTG CCAGCTGTCA
    TCTGAGTCTC CTGCTAAAGT GACATGGATG CTTGATGGAA AAGTCATCAG ACCCTCCAAA
    TTCATCATTA TCACTAATGA GGGTGATCGG TACTCTTTAT CTATTGATAA AGCTCTGCCT
    GAGGATGAAG GGCAGTACGT GTGCAGGGCA GAGACCGATG AGGGCAAGAC TGAGTGTTCC
    TGTGTGGTCC TAGTGAACGA TCCCAGCGTC TCCACACCAG ATGATAAGCG GAGCAGGAAG
    CCTTCTACCC CGACCACAGA GAATGAGGCC AAAATAAAGA AACCCCCACC CAAAACACTG
    CCTAAACAAG GTGCTCCGCC TCAAATCCAG CAGTTTCCTG ATGATATGAA GATCATGGCA
    GGTCAGGAGG TGATTCTGCA GTGTAAGTTC ACTGGAGCAC TCCCCATCAG CTGCACCTGG
    CTCAAGTTCC GCAAACCAAT TCAGGAAGGT CAGGGCATCT CAATAGTCAG CACAGACAGT
    AGCAGTCAGC TGACCATCAC AGAAGGACAG CAGGAGCACT GTGGCTGCTA TACCATAGAG
    ATCAAGAACA GCTTTGGTGT GAGACAGGCT GCCCTCAACC TCACTATAGT CGATAAGCCT
    GACCCCCCTG CCCGTGTCCC AGCAGCCTCA GATATCCGTA AGTCCAGCCT CACGCTGTCC
    TGGTATGGGC CAACATACGA CGGTGGCAGC GCTGTCCAAT CCTACAACCT GGAGGCTTGG
    AACTCTCTGG ACATGAAGTG GACGGACGTG GCATCCTGTA ACAGCACATC TTATAACGTG
    CAGGACCTTC TTCCAGACAG ACAGTACAAG TTTCGAGTGA GAGCTGTAAA TATTTATGGC
    GTCGGGGAGC CCAGTGCCGA GTCTGAGCCC GTGCAGGTGG GGCTGGAGGC AGAGGGGGAA
    AATAAGGAGG ATGAAGTCGA GCCCTCGGAT GATGAGTCAG AGAAAGTACC AGACTACAGA
    AATGTGACGA TCAAAACAGA CGTAAAAGTA AAAGATTTTT ATGATGTAGA AGACCGACTG
    GGCACAGGGA AATTTGGGAC CGTTTTCAAA CTGATCGAGA AGTCCACTAA AAAGGTTTGG
    GCTGGGAAAT TCATAAAAGC ATATTCTGCC AAAGAAAAAG AAAACGTCAG GCAGGAAATT
    GCAATCATGA ATGACCTGCA TCATCCAAAG CTGGTGCAGT GCATCGATGC TTTTGAGGGA
    AAGACCGACA TCGTAATGGT GCTGGAAATG ATCTCTGGAG GGGAACTGTT TGACCGGATC
    ATAGATGAGG ACTTTGAGCT GACGGAGAGA GAGGTGATCA AATATATGCT TCAGATTGTG
    GACGGGGTCA ATTTCATCCA TAAGAAAGGC ATCGTCCACC TGGACCTCAA ACCTGAGAAC
    ATCATGTGTG TTAATAAGAC GGGCAGCAAA ATCAAACTCA TCGACTTTGG ACTGGCACGC
    AGACTTGAGA ATGCTGGATC TCTCAAAGTC CTGTTTGGAA CCCCTGAGTT TGTGGCTCCA
    GAGGTGATCA ATTATGAGGC CATCAGTTAT GCGACAGACA TGTGGAGTAT AGGAGTGATC
    TGCTACATCC TTGTCAGTGG TTTGTCTCCG TTCATGGGGG ACAATGATAA CGAGACGCTG
    TCAAATGTGA CCTCAGCGAC GTGGGACTTT GAAGACGAGG CATTTGATGA GATCTCAGAA
    GAGGCCAAAG ACTTTATCAG TAATCTGCTA AAGAAAGACA TGAAAGCTCG CCTGACCTGT
    GACCAGTGTT TCCAGCATCC ATGGCTTAAA CAAGACACCA CCAACATGGA GGCCAAGAAA
    CTTTCCAAAG AGAGGATGAA GAAGTATATT CTGAGAAGAA AGTGGCAGAA AACAGGGCAT
    GCGGTACGAG CTATTGGTCG ACTGTCTAAC ATGGCATTGA TGGCAGGTGT TCATGCCAAA
    AAAAGTTCCC CCACAGAAGC AGATGCTAAA CAGTTTGCTG CTGAATTGGA GGCAAAAACT
    TTGATGAAGC CCACGTTCAG CTCTGTTATC AAAGATGTGG AGGTGGTGGA GGGAAGCGCT
    GCTCGATTTG ACTGCAAAAT TGAAGGTTAT CCTGACCCAG AGGTGGTGTG GTATAAGGAC
    GATCAGCCCA TTAAAGAGAC GCGCCATTTT CAGATAGATT ATGACGAGGA CGGAAACTGT
    AGTCTGGTCA TTTCTGAGGT CAACGGCGAT GATGACGCAA AATATACCTG CAAGGCCATG
    AACAGCCTCG GGGAGACCAC CTGCACCGCT GAACTCATCG TAGAGGTGAT GGGAGAAGAA
    GAAGAGGAGG AAGAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45772
    Clone ID Related Accession (Same CDS sequence) XM_009304445.1
    Accession Version XM_009304445.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5574bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product myosin light chain kinase, smooth muscle isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGCAGCT CTCAGCCTGT GATGTTAGGA TTCATGCCAG CAAAGTTTGG TGCCGACAGG 
    GAAAACACGT TAAACGCAGA GTGGAGTCGG GACAGCGGGG TCTGGACGCG CTCTCGACAC
    TGCACTCCGG TGAATGACAC TAATCTGCAC TGCTGTCTAC ATGGAATGAT GGCCGATGTT
    CAGTTTGTTA CAGGATCTCA TGTCTCCAAG ACAGCTCTGA CTCTGGGACG AGGTTCCCCC
    AGCAAAGAGC CCCCCGTTTT TACCCTCCCG CCCCGCAATA CCCGTGTCTG CCAAGGGGGA
    ACTGCGCGCC TTGAGGGGAA GGTACGTGGT TTCCCAGAGC CTCAGGTATG CTGGTTTAGG
    AATGGCAAGC TACTGATAGC AGGAGATCAT TATTCTATGG AACAGAGTGT CCGTGGAACA
    TTTAGTCTGG TGGTCCAGGA GGTAGAGGAT GCCGATGGAG GACGTTACAC CTGTGAAGCT
    GTTAACGATG CTGGGAGCTG CCAGGTTACT GTGGAGATCA TTGTGGAAGG AAACACAGGA
    AAGAAATACA GTCTTCCTTC CACAAACAGA ACAGGCAGAT CTATGGGAGC TCCCGCAGTA
    GAAAACAGAG CCAGTATCTG GGGTGAAAGC CCCCCAAAGT TTGTGGCTAA ACCTTCTCGC
    CTGTATCTCA AAGTGGGACA AACTGGCAAA TTTTCGGCCA AGATCACAGG ACGACCCCAG
    CCACTGGTGC AGTGGTACAA GGGAGAAGAG ATGATCCAGC AATCAGATCA CTATAACATG
    TTTGAGAGGT CAGGAATGCA TTTCCTGGAG ATCCGCAATG TGTTCTCTGA GGATGCTGGC
    ATGTACACCT GTCTGGTGGC CAACAGTGCT GGAAAAGCCT CTGCAGCCGC TGAACTCATT
    ATGCAAGATG GAGACACCGA GACAGTCATC ATGGCCAAAC CATCTCACAA TCCCTCCAAG
    TCATTCATGC TCAAAGACAC TTCCAACACT CTTGCCAACG AACAGAAAAC TTCCAGCACA
    TCCATCGAAA CCATAATCAC AGAAACACCC ACACCGCTGT CCACTACTAC TGTTCCCACT
    GTCCCATCCA TGAACAGAGG AGAAACAACA ATAGTAACAT CATCACTTCA CTCACAACCT
    ATCAGCAGGT CCACAGATGG AAGATCAGCA ACTGGCCAAA ATACTTCAAA CTTAGTCAAT
    CTAAGAAGTC AAAACAGTGT TAAGAATGAG ACGCATGGCA CAAAGAGAGT TTTGGGTATG
    TTCAGAGCTC CGGGACAGAT GCCACAATCA TCACAGATAG AATCCAGCAC TCGCCAACAA
    ACCAAAGAAA CCAGAATGGA TGTGCTAGAA ACTCCACCCA GTTTTGACTC GCAGCCCCAG
    AGCCAAGAGG TCCTGCAGGG TGAAAAGGTC ACTTTCAAAT GCCAAGTGTC CGGGTCTCCA
    GTGCCTGCAG TGAGCTGGCT GAAAGATGGA GCTCCTCTCA GACAGAGATC AGATGTCAGC
    ACTCGACAGG AGGCCGGCCT GCATGTTCTG TGTCTGGACA ATGCTCACAT AACTGACACG
    GGACAGTATG GCTGCACTGC TGCCAACAGA AGAGGAAACA CCGCGGCCCA CTGGAGTCTG
    ACCGTCAAGC GTGTGCGAGT GGATGCGAAG CCCCCAGTGT TCAGCTCTGC TCTGAAGGAT
    TGTTCGGTGT GTGAAGGACA GGAATTTACC CTGCAGTGTT CAGTGGAGGG CAAACCACAG
    CCTGAGCTCT CCTGGCTGCA CAACGATAAG CCCATTCCAT TCTCCCATAC CATGTTTGAC
    AACAGTAAAG CTCAGCTGAG AGTTCAGGCG GCTGTATTGG AGGATGCAGG CGTTTATTCG
    TGTGTAGCAG TGAATGAGCA TGGCAGAGCG GTGTGTACAG CCCATGTGCT GGTTAAAGCA
    AGGCCAAGGG TGAAAAGGTC TGCTGCTCCT CCTGTTACTG ATCTGAGGAG AACATCTGCT
    CCGCTGTTCC TCAAAGGTCT ACAGGACCTC AGGGTGATGG ACGGCAGTCA GGTCATCATG
    ACCGTGGAAG TGACAGGAAA CCCTGCACCT GAGGTGGTCT GGCTGCATAA TGGAAAAGAG
    ATCCAGGAGT CAGAGGACTT TCACTTTGAA AAGAAGGGCG GTCAGTATAC ACTCTTCATA
    CAGGAGGTGT TTCCTGAGGA CACGGGGACA TACACTTGTG AGGTGTGGAA TGAAGCCGGC
    AACGCTCGAA CTCAAGCTTC ACTCACTGTA CAAGAGCCTC AAGATGGTGT TCAGCCCTGG
    TTCATTACTA AAACCAAGTC CTCCACCGCC TCTCTGGGTC AACATGCTCT GCTCTCTTGC
    GCCATTGCTG GTGACCCCTT CCCAGAATTC CTCTGGATGA AGGATGGACA GGTAATATCT
    GGTGATGGGG ACTTTGAGAT CCTACAGAAG GAAGATGTTG TCTCGCTGCT TATCAGGAAA
    GTGGCGATGC ATCACGCTGG AAACTACGAG ATTCATCTTA AAAATAAAGT AGGAGAGTGC
    CGGTCAGTGG CCACTCTGCA TGTCAGCGAG GATCCCATGC TGTCTGCTGA TGATGCTAAC
    AAACGCCGAG GGGTGGAGAA GACTGCGCTA GGCAGGGGAG GACAGCCAAA GACTCCCAGC
    CCTGCAGCAT CGTTGCACTC TGAGGAACAT AGCAGTGGGA CAGATCAGCA AGAAGAGGAG
    GGGGGCTCCA CGCGTGGGCT TCTCAAACGG CGTGTAGAAA CGAAGGAGCA TCGGGAAGAT
    CAGATCCGAC AGCAGGAGGC CGAACAGATC GACTTCAGAA CCGTGCTCGG CCGCAAGGTC
    ACCACTAAGA GCGTCTCAGA GGAAGATCTG AAGGAGATCA CGGCTGAGCA GATGGACTTC
    AGAGGGAACC TCCAACGGCA GATCAAGCCC AAGACTCAGA CAGAAGAGCA ACGCAAAGTC
    AACACTCCGC AGCAGGTGGA CTTCAGAGCG GTGCTAGGGA AAAAAGCAAG CCAAGGCCCA
    AAACCTGCTC CTGGATCCTT GAAAACGCAA CCTAGCAAAA ACGAAGCCGT TGACTTCAGA
    TCTGTTTTAG GCAATAAGAA AAAGCCACCA ACCCAGGATA GGAATGGAGA AAGCCAAGGC
    GACCCCAGAG GAAAGGAAAA TGACGTGAAT TGTGTGGATG GAGAGATAAA GGAGACCACG
    AAGGCAGCTG GGAAGAAGGA GCCTGTTTTT GTAGAGAAAC TGAGTGATGT GACTGTGCTG
    GACGGAGAGC GTTTGCATCT ACAGTGCCAG CTGTCATCTG AGTCTCCTGC TAAAGTGACA
    TGGATGCTTG ATGGAAAAGT CATCAGACCC TCCAAATTCA TCATTATCAC TAATGAGGGT
    GATCGGTACT CTTTATCTAT TGATAAAGCT CTGCCTGAGG ATGAAGGGCA GTACGTGTGC
    AGGGCAGAGA CCGATGAGGG CAAGACTGAG TGTTCCTGTG TGGTCCTAGT GAACGATCCC
    AGCGTCTCCA CACCAGATGA TAAGCGGAGC AGGAAGCCTT CTACCCCGAC CACAGAGAAT
    GAGGCCAAAA TAAAGAAACC CCCACCCAAA ACACTGCCTA AACAAGGTGC TCCGCCTCAA
    ATCCAGCAGT TTCCTGATGA TATGAAGATC ATGGCAGGTC AGGAGGTGAT TCTGCAGTGT
    AAGTTCACTG GAGCACTCCC CATCAGCTGC ACCTGGCTCA AGTTCCGCAA ACCAATTCAG
    GAAGGTCAGG GCATCTCAAT AGTCAGCACA GACAGTAGCA GTCAGCTGAC CATCACAGAA
    GGACAGCAGG AGCACTGTGG CTGCTATACC ATAGAGATCA AGAACAGCTT TGGTGTGAGA
    CAGGCTGCCC TCAACCTCAC TATAGTCGAT AAGCCTGACC CCCCTGCCCG TGTCCCAGCA
    GCCTCAGATA TCCGTAAGTC CAGCCTCACG CTGTCCTGGT ATGGGCCAAC ATACGACGGT
    GGCAGCGCTG TCCAATCCTA CAACCTGGAG GCTTGGAACT CTCTGGACAT GAAGTGGACG
    GACGTGGCAT CCTGTAACAG CACATCTTAT AACGTGCAGG ACCTTCTTCC AGACAGACAG
    TACAAGTTTC GAGTGAGAGC TGTAAATATT TATGGCGTCG GGGAGCCCAG TGCCGAGTCT
    GAGCCCGTGC AGGTGGGGCT GGAGGCAGAG GGGGAAAATA AGGAGGATGA AGTCGAGCCC
    TCGGATGATG AGTCAGAGAA AGTACCAGAC TACAGAAATG TGACGATCAA AACAGACGTA
    AAAGTAAAAG ATTTTTATGA TGTAGAAGAC CGACTGGGCA CAGGGAAATT TGGGACCGTT
    TTCAAACTGA TCGAGAAGTC CACTAAAAAG GTTTGGGCTG GGAAATTCAT AAAAGCATAT
    TCTGCCAAAG AAAAAGAAAA CGTCAGGCAG GAAATTGCAA TCATGAATGA CCTGCATCAT
    CCAAAGCTGG TGCAGTGCAT CGATGCTTTT GAGGGAAAGA CCGACATCGT AATGGTGCTG
    GAAATGATCT CTGGAGGGGA ACTGTTTGAC CGGATCATAG ATGAGGACTT TGAGCTGACG
    GAGAGAGAGG TGATCAAATA TATGCTTCAG ATTGTGGACG GGGTCAATTT CATCCATAAG
    AAAGGCATCG TCCACCTGGA CCTCAAACCT GAGAACATCA TGTGTGTTAA TAAGACGGGC
    AGCAAAATCA AACTCATCGA CTTTGGACTG GCACGCAGAC TTGAGAATGC TGGATCTCTC
    AAAGTCCTGT TTGGAACCCC TGAGTTTGTG GCTCCAGAGG TGATCAATTA TGAGGCCATC
    AGTTATGCGA CAGACATGTG GAGTATAGGA GTGATCTGCT ACATCCTTGT CAGTGGTTTG
    TCTCCGTTCA TGGGGGACAA TGATAACGAG ACGCTGTCAA ATGTGACCTC AGCGACGTGG
    GACTTTGAAG ACGAGGCATT TGATGAGATC TCAGAAGAGG CCAAAGACTT TATCAGTAAT
    CTGCTAAAGA AAGACATGAA AGCTCGCCTG ACCTGTGACC AGTGTTTCCA GCATCCATGG
    CTTAAACAAG ACACCACCAA CATGGAGGCC AAGAAACTTT CCAAAGAGAG GATGAAGAAG
    TATATTCTGA GAAGAAAGTG GCAGAAAACA GGGCATGCGG TACGAGCTAT TGGTCGACTG
    TCTAACATGG CATTGATGGC AGGTGTTCAT GCCAAAAAAA GTTCCCCCAC AGAAGCAGAT
    GCTAAACAGT TTGCTGCTGA ATTGGAGGCA AAAACTTTGA TGAAGCCCAC GTTCAGCTCT
    GTTATCAAAG ATGTGGAGGT GGTGGAGGGA AGCGCTGCTC GATTTGACTG CAAAATTGAA
    GGTTATCCTG ACCCAGAGGT GGTGTGGTAT AAGGACGATC AGCCCATTAA AGAGACGCGC
    CATTTTCAGA TAGATTATGA CGAGGACGGA AACTGTAGTC TGGTCATTTC TGAGGTCAAC
    GGCGATGATG ACGCAAAATA TACCTGCAAG GCCATGAACA GCCTCGGGGA GACCACCTGC
    ACCGCTGAAC TCATCGTAGA GGTGATGGGA GAAGAAGAAG AGGAGGAAGA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302720.1
    CDS36..5609
    Translation

    Target ORF information:

    RefSeq Version XM_009304445.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio myosin, light chain kinase a (mylka), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304445.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGCAGCT CTCAGCCTGT GATGTTAGGA TTCATGCCAG CAAAGTTTGG TGCCGACAGG 
    GAAAACACGT TAAACGCAGA GTGGAGTCGG GACAGCGGGG TCTGGACGCG CTCTCGACAC
    TGCACTCCGG TGAATGACAC TAATCTGCAC TGCTGTCTAC ATGGAATGAT GGCCGATGTT
    CAGTTTGTTA CAGGATCTCA TGTCTCCAAG ACAGCTCTGA CTCTGGGACG AGGTTCCCCC
    AGCAAAGAGC CCCCCGTTTT TACCCTCCCG CCCCGCAATA CCCGTGTCTG CCAAGGGGGA
    ACTGCGCGCC TTGAGGGGAA GGTACGTGGT TTCCCAGAGC CTCAGGTATG CTGGTTTAGG
    AATGGCAAGC TACTGATAGC AGGAGATCAT TATTCTATGG AACAGAGTGT CCGTGGAACA
    TTTAGTCTGG TGGTCCAGGA GGTAGAGGAT GCCGATGGAG GACGTTACAC CTGTGAAGCT
    GTTAACGATG CTGGGAGCTG CCAGGTTACT GTGGAGATCA TTGTGGAAGG AAACACAGGA
    AAGAAATACA GTCTTCCTTC CACAAACAGA ACAGGCAGAT CTATGGGAGC TCCCGCAGTA
    GAAAACAGAG CCAGTATCTG GGGTGAAAGC CCCCCAAAGT TTGTGGCTAA ACCTTCTCGC
    CTGTATCTCA AAGTGGGACA AACTGGCAAA TTTTCGGCCA AGATCACAGG ACGACCCCAG
    CCACTGGTGC AGTGGTACAA GGGAGAAGAG ATGATCCAGC AATCAGATCA CTATAACATG
    TTTGAGAGGT CAGGAATGCA TTTCCTGGAG ATCCGCAATG TGTTCTCTGA GGATGCTGGC
    ATGTACACCT GTCTGGTGGC CAACAGTGCT GGAAAAGCCT CTGCAGCCGC TGAACTCATT
    ATGCAAGATG GAGACACCGA GACAGTCATC ATGGCCAAAC CATCTCACAA TCCCTCCAAG
    TCATTCATGC TCAAAGACAC TTCCAACACT CTTGCCAACG AACAGAAAAC TTCCAGCACA
    TCCATCGAAA CCATAATCAC AGAAACACCC ACACCGCTGT CCACTACTAC TGTTCCCACT
    GTCCCATCCA TGAACAGAGG AGAAACAACA ATAGTAACAT CATCACTTCA CTCACAACCT
    ATCAGCAGGT CCACAGATGG AAGATCAGCA ACTGGCCAAA ATACTTCAAA CTTAGTCAAT
    CTAAGAAGTC AAAACAGTGT TAAGAATGAG ACGCATGGCA CAAAGAGAGT TTTGGGTATG
    TTCAGAGCTC CGGGACAGAT GCCACAATCA TCACAGATAG AATCCAGCAC TCGCCAACAA
    ACCAAAGAAA CCAGAATGGA TGTGCTAGAA ACTCCACCCA GTTTTGACTC GCAGCCCCAG
    AGCCAAGAGG TCCTGCAGGG TGAAAAGGTC ACTTTCAAAT GCCAAGTGTC CGGGTCTCCA
    GTGCCTGCAG TGAGCTGGCT GAAAGATGGA GCTCCTCTCA GACAGAGATC AGATGTCAGC
    ACTCGACAGG AGGCCGGCCT GCATGTTCTG TGTCTGGACA ATGCTCACAT AACTGACACG
    GGACAGTATG GCTGCACTGC TGCCAACAGA AGAGGAAACA CCGCGGCCCA CTGGAGTCTG
    ACCGTCAAGC GTGTGCGAGT GGATGCGAAG CCCCCAGTGT TCAGCTCTGC TCTGAAGGAT
    TGTTCGGTGT GTGAAGGACA GGAATTTACC CTGCAGTGTT CAGTGGAGGG CAAACCACAG
    CCTGAGCTCT CCTGGCTGCA CAACGATAAG CCCATTCCAT TCTCCCATAC CATGTTTGAC
    AACAGTAAAG CTCAGCTGAG AGTTCAGGCG GCTGTATTGG AGGATGCAGG CGTTTATTCG
    TGTGTAGCAG TGAATGAGCA TGGCAGAGCG GTGTGTACAG CCCATGTGCT GGTTAAAGCA
    AGGCCAAGGG TGAAAAGGTC TGCTGCTCCT CCTGTTACTG ATCTGAGGAG AACATCTGCT
    CCGCTGTTCC TCAAAGGTCT ACAGGACCTC AGGGTGATGG ACGGCAGTCA GGTCATCATG
    ACCGTGGAAG TGACAGGAAA CCCTGCACCT GAGGTGGTCT GGCTGCATAA TGGAAAAGAG
    ATCCAGGAGT CAGAGGACTT TCACTTTGAA AAGAAGGGCG GTCAGTATAC ACTCTTCATA
    CAGGAGGTGT TTCCTGAGGA CACGGGGACA TACACTTGTG AGGTGTGGAA TGAAGCCGGC
    AACGCTCGAA CTCAAGCTTC ACTCACTGTA CAAGAGCCTC AAGATGGTGT TCAGCCCTGG
    TTCATTACTA AAACCAAGTC CTCCACCGCC TCTCTGGGTC AACATGCTCT GCTCTCTTGC
    GCCATTGCTG GTGACCCCTT CCCAGAATTC CTCTGGATGA AGGATGGACA GGTAATATCT
    GGTGATGGGG ACTTTGAGAT CCTACAGAAG GAAGATGTTG TCTCGCTGCT TATCAGGAAA
    GTGGCGATGC ATCACGCTGG AAACTACGAG ATTCATCTTA AAAATAAAGT AGGAGAGTGC
    CGGTCAGTGG CCACTCTGCA TGTCAGCGAG GATCCCATGC TGTCTGCTGA TGATGCTAAC
    AAACGCCGAG GGGTGGAGAA GACTGCGCTA GGCAGGGGAG GACAGCCAAA GACTCCCAGC
    CCTGCAGCAT CGTTGCACTC TGAGGAACAT AGCAGTGGGA CAGATCAGCA AGAAGAGGAG
    GGGGGCTCCA CGCGTGGGCT TCTCAAACGG CGTGTAGAAA CGAAGGAGCA TCGGGAAGAT
    CAGATCCGAC AGCAGGAGGC CGAACAGATC GACTTCAGAA CCGTGCTCGG CCGCAAGGTC
    ACCACTAAGA GCGTCTCAGA GGAAGATCTG AAGGAGATCA CGGCTGAGCA GATGGACTTC
    AGAGGGAACC TCCAACGGCA GATCAAGCCC AAGACTCAGA CAGAAGAGCA ACGCAAAGTC
    AACACTCCGC AGCAGGTGGA CTTCAGAGCG GTGCTAGGGA AAAAAGCAAG CCAAGGCCCA
    AAACCTGCTC CTGGATCCTT GAAAACGCAA CCTAGCAAAA ACGAAGCCGT TGACTTCAGA
    TCTGTTTTAG GCAATAAGAA AAAGCCACCA ACCCAGGATA GGAATGGAGA AAGCCAAGGC
    GACCCCAGAG GAAAGGAAAA TGACGTGAAT TGTGTGGATG GAGAGATAAA GGAGACCACG
    AAGGCAGCTG GGAAGAAGGA GCCTGTTTTT GTAGAGAAAC TGAGTGATGT GACTGTGCTG
    GACGGAGAGC GTTTGCATCT ACAGTGCCAG CTGTCATCTG AGTCTCCTGC TAAAGTGACA
    TGGATGCTTG ATGGAAAAGT CATCAGACCC TCCAAATTCA TCATTATCAC TAATGAGGGT
    GATCGGTACT CTTTATCTAT TGATAAAGCT CTGCCTGAGG ATGAAGGGCA GTACGTGTGC
    AGGGCAGAGA CCGATGAGGG CAAGACTGAG TGTTCCTGTG TGGTCCTAGT GAACGATCCC
    AGCGTCTCCA CACCAGATGA TAAGCGGAGC AGGAAGCCTT CTACCCCGAC CACAGAGAAT
    GAGGCCAAAA TAAAGAAACC CCCACCCAAA ACACTGCCTA AACAAGGTGC TCCGCCTCAA
    ATCCAGCAGT TTCCTGATGA TATGAAGATC ATGGCAGGTC AGGAGGTGAT TCTGCAGTGT
    AAGTTCACTG GAGCACTCCC CATCAGCTGC ACCTGGCTCA AGTTCCGCAA ACCAATTCAG
    GAAGGTCAGG GCATCTCAAT AGTCAGCACA GACAGTAGCA GTCAGCTGAC CATCACAGAA
    GGACAGCAGG AGCACTGTGG CTGCTATACC ATAGAGATCA AGAACAGCTT TGGTGTGAGA
    CAGGCTGCCC TCAACCTCAC TATAGTCGAT AAGCCTGACC CCCCTGCCCG TGTCCCAGCA
    GCCTCAGATA TCCGTAAGTC CAGCCTCACG CTGTCCTGGT ATGGGCCAAC ATACGACGGT
    GGCAGCGCTG TCCAATCCTA CAACCTGGAG GCTTGGAACT CTCTGGACAT GAAGTGGACG
    GACGTGGCAT CCTGTAACAG CACATCTTAT AACGTGCAGG ACCTTCTTCC AGACAGACAG
    TACAAGTTTC GAGTGAGAGC TGTAAATATT TATGGCGTCG GGGAGCCCAG TGCCGAGTCT
    GAGCCCGTGC AGGTGGGGCT GGAGGCAGAG GGGGAAAATA AGGAGGATGA AGTCGAGCCC
    TCGGATGATG AGTCAGAGAA AGTACCAGAC TACAGAAATG TGACGATCAA AACAGACGTA
    AAAGTAAAAG ATTTTTATGA TGTAGAAGAC CGACTGGGCA CAGGGAAATT TGGGACCGTT
    TTCAAACTGA TCGAGAAGTC CACTAAAAAG GTTTGGGCTG GGAAATTCAT AAAAGCATAT
    TCTGCCAAAG AAAAAGAAAA CGTCAGGCAG GAAATTGCAA TCATGAATGA CCTGCATCAT
    CCAAAGCTGG TGCAGTGCAT CGATGCTTTT GAGGGAAAGA CCGACATCGT AATGGTGCTG
    GAAATGATCT CTGGAGGGGA ACTGTTTGAC CGGATCATAG ATGAGGACTT TGAGCTGACG
    GAGAGAGAGG TGATCAAATA TATGCTTCAG ATTGTGGACG GGGTCAATTT CATCCATAAG
    AAAGGCATCG TCCACCTGGA CCTCAAACCT GAGAACATCA TGTGTGTTAA TAAGACGGGC
    AGCAAAATCA AACTCATCGA CTTTGGACTG GCACGCAGAC TTGAGAATGC TGGATCTCTC
    AAAGTCCTGT TTGGAACCCC TGAGTTTGTG GCTCCAGAGG TGATCAATTA TGAGGCCATC
    AGTTATGCGA CAGACATGTG GAGTATAGGA GTGATCTGCT ACATCCTTGT CAGTGGTTTG
    TCTCCGTTCA TGGGGGACAA TGATAACGAG ACGCTGTCAA ATGTGACCTC AGCGACGTGG
    GACTTTGAAG ACGAGGCATT TGATGAGATC TCAGAAGAGG CCAAAGACTT TATCAGTAAT
    CTGCTAAAGA AAGACATGAA AGCTCGCCTG ACCTGTGACC AGTGTTTCCA GCATCCATGG
    CTTAAACAAG ACACCACCAA CATGGAGGCC AAGAAACTTT CCAAAGAGAG GATGAAGAAG
    TATATTCTGA GAAGAAAGTG GCAGAAAACA GGGCATGCGG TACGAGCTAT TGGTCGACTG
    TCTAACATGG CATTGATGGC AGGTGTTCAT GCCAAAAAAA GTTCCCCCAC AGAAGCAGAT
    GCTAAACAGT TTGCTGCTGA ATTGGAGGCA AAAACTTTGA TGAAGCCCAC GTTCAGCTCT
    GTTATCAAAG ATGTGGAGGT GGTGGAGGGA AGCGCTGCTC GATTTGACTG CAAAATTGAA
    GGTTATCCTG ACCCAGAGGT GGTGTGGTAT AAGGACGATC AGCCCATTAA AGAGACGCGC
    CATTTTCAGA TAGATTATGA CGAGGACGGA AACTGTAGTC TGGTCATTTC TGAGGTCAAC
    GGCGATGATG ACGCAAAATA TACCTGCAAG GCCATGAACA GCCTCGGGGA GACCACCTGC
    ACCGCTGAAC TCATCGTAGA GGTGATGGGA GAAGAAGAAG AGGAGGAAGA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45773
    Clone ID Related Accession (Same CDS sequence) XM_009304446.1
    Accession Version XM_009304446.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5571bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product myosin light chain kinase, smooth muscle isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGCAGCT CTCAGCCTGT GATGTTAGGA TTCATGCCAG CAAAGTTTGG TGCCGACAGG 
    GAAAACACGT TAAACGCAGA GTGGAGTCGG GACAGCGGGG TCTGGACGCG CTCTCGACAC
    TGCACTCCGG TGAATGACAC TAATCTGCAC TGCTGTCTAC ATGGAATGAT GGCCGATGTT
    CAGTTTGTTA CAGGATCTCA TGTCTCCAAG ACAGCTCTGA CTCTGGGACG AGGTTCCCCC
    AGCAAAGAGC CCCCCGTTTT TACCCTCCCG CCCCGCAATA CCCGTGTCTG CCAAGGGGGA
    ACTGCGCGCC TTGAGGGGAA GGTACGTGGT TTCCCAGAGC CTCAGGTATG CTGGTTTAGG
    AATGGCAAGC TACTGATAGC AGGAGATCAT TATTCTATGG AACAGAGTGT CCGTGGAACA
    TTTAGTCTGG TGGTCCAGGA GGTAGAGGAT GCCGATGGAG GACGTTACAC CTGTGAAGCT
    GTTAACGATG CTGGGAGCTG CCAGGTTACT GTGGAGATCA TTGTGGAAGG AAACACAGGA
    AAGAAATACA GTCTTCCTTC CACAAACAGA ACAGGCAGAT CTATGGGAGC TCCCGCAGTA
    GAAAACAGAG CCAGTATCTG GGGTGAAAGC CCCCCAAAGT TTGTGGCTAA ACCTTCTCGC
    CTGTATCTCA AAGTGGGACA AACTGGCAAA TTTTCGGCCA AGATCACAGG ACGACCCCAG
    CCACTGGTGC AGTGGTACAA GGGAGAAGAG ATGATCCAGC AATCAGATCA CTATAACATG
    TTTGAGAGGT CAGGAATGCA TTTCCTGGAG ATCCGCAATG TGTTCTCTGA GGATGCTGGC
    ATGTACACCT GTCTGGTGGC CAACAGTGCT GGAAAAGCCT CTGCAGCCGC TGAACTCATT
    ATGCAAGATG GAGACACCGA GACAGTCATC ATGGCCAAAC CATCTCACAA TCCCTCCAAG
    TCATTCATGC TCAAAGACAC TTCCAACACT CTTGCCAACG AACAGAAAAC TTCCAGCACA
    TCCATCGAAA CCATAATCAC AGAAACACCC ACACCGCTGT CCACTACTAC TGTTCCCACT
    GTCCCATCCA TGAACAGAGG AGAAACAACA ATAGTAACAT CATCACTTCA CTCACAACCT
    ATCAGCAGGT CCACAGATGG AAGATCAGCA ACTGGCCAAA ATACTTCAAA CTTAGTCAAT
    CTAAGAAGTC AAAACAGTGT TAAGAATGAG ACGCATGGCA CAAAGAGAGT TTTGGGTATG
    TTCAGAGCTC CGGGACAGAT GCCACAATCA TCACAGATAG AATCCAGCAC TCGCCAACAA
    ACCAAAGAAA CCAGAATGGA TGTGCTAGAA ACTCCACCCA GTTTTGACTC GCAGCCCCAG
    AGCCAAGAGG TCCTGCAGGG TGAAAAGGTC ACTTTCAAAT GCCAAGTGTC CGGGTCTCCA
    GTGCCTGCAG TGAGCTGGCT GAAAGATGGA GCTCCTCTCA GACAGAGATC AGATGTCAGC
    ACTCGACAGG AGGCCGGCCT GCATGTTCTG TGTCTGGACA ATGCTCACAT AACTGACACG
    GGACAGTATG GCTGCACTGC TGCCAACAGA AGAGGAAACA CCGCGGCCCA CTGGAGTCTG
    ACCGTCAAGC GTGTGCGAGT GGATGCGAAG CCCCCAGTGT TCAGCTCTGC TCTGAAGGAT
    TGTTCGGTGT GTGAAGGACA GGAATTTACC CTGCAGTGTT CAGTGGAGGG CAAACCACAG
    CCTGAGCTCT CCTGGCTGCA CAACGATAAG CCCATTCCAT TCTCCCATAC CATGTTTGAC
    AACAGTAAAG CTCAGCTGAG AGTTCAGGCG GCTGTATTGG AGGATGCAGG CGTTTATTCG
    TGTGTAGCAG TGAATGAGCA TGGCAGAGCG GTGTGTACAG CCCATGTGCT GGTTAAAGCA
    AGGCCAAGGG TGAAAAGGTC TGCTGCTCCT CCTGTTACTG ATCTGAGGAG AACATCTGCT
    CCGCTGTTCC TCAAAGGTCT ACAGGACCTC AGGGTGATGG ACGGCAGTCA GGTCATCATG
    ACCGTGGAAG TGACAGGAAA CCCTGCACCT GAGGTGGTCT GGCTGCATAA TGGAAAAGAG
    ATCCAGGAGT CAGAGGACTT TCACTTTGAA AAGAAGGGCG GTCAGTATAC ACTCTTCATA
    CAGGAGGTGT TTCCTGAGGA CACGGGGACA TACACTTGTG AGGTGTGGAA TGAAGCCGGC
    AACGCTCGAA CTCAAGCTTC ACTCACTGTA CAAGAGCCTC AAGATGGTGT TCAGCCCTGG
    TTCATTACTA AAACCAAGTC CTCCACCGCC TCTCTGGGTC AACATGCTCT GCTCTCTTGC
    GCCATTGCTG GTGACCCCTT CCCAGAATTC CTCTGGATGA AGGATGGACA GGTAATATCT
    GGTGATGGGG ACTTTGAGAT CCTACAGAAG GAAGATGTTG TCTCGCTGCT TATCAGGAAA
    GTGGCGATGC ATCACGCTGG AAACTACGAG ATTCATCTTA AAAATAAAGT AGGAGAGTGC
    CGGTCAGTGG CCACTCTGCA TGTCAGCGAG GATCCCATGC TGTCTGCTGA TGATGCTAAC
    AAACGCCGAG GGGTGGAGAA GACTGCGCTA GGCAGGGGAG GACAGCCAAA GACTCCCAGC
    CCTGCAGCAT CGTTGCACTC TGAGGAACAT AGCAGTGGGA CAGATCAGCA AGAAGAGGAG
    GGGGGCTCCA CGCGTGGGCT TCTCAAACGG CGTGTAGAAA CGAAGGAGCA TCGGGAAGAT
    CAGATCCGAC AGCAGGAGGC CGAACAGATC GACTTCAGAA CCGTGCTCGG CCGCAAGGTC
    ACCACTAAGA GCGTCTCAGA GGAAGATCTG AAGGAGATCA CGGCTGAGCA GATGGACTTC
    AGAGGGAACC TCCAACGGCA GATCAAGCCC AAGACTCAGA CAGAAGAGCA ACGCAAAGTC
    AACACTCCGC AGCAGGTGGA CTTCAGAGCG GTGCTAGGGA AAAAAGCAAG CCAAGGCCCA
    AAACCTGCTC CTGGATCCTT GAAAACGCAA CCTAGCAAAA ACGAAGCCGT TGACTTCAGA
    TCTGTTTTAG GCAATAAGAA AAAGCCACCA ACCCAGGATA GGAATGGAGA AAGCCAAGGC
    GACCCCAGAG GAAAGGAAAA TGACGTGAAT TGTGTGGATG GAGAGATAAA GGAGACCACG
    AAGGCAGCTG GGAAGAAGGA GCCTGTTTTT GTAGAGAAAC TGAGTGATGT GACTGTGCTG
    GACGGAGAGC GTTTGCATCT ACAGTGCCAG CTGTCATCTG AGTCTCCTGC TAAAGTGACA
    TGGATGCTTG ATGGAAAAGT CATCAGACCC TCCAAATTCA TCATTATCAC TAATGAGGGT
    GATCGGTACT CTTTATCTAT TGATAAAGCT CTGCCTGAGG ATGAAGGGCA GTACGTGTGC
    AGGGCAGAGA CCGATGAGGG CAAGACTGAG TGTTCCTGTG TGGTCCTAGT GAACGATCCC
    AGCGTCTCCA CACCAGATGA TAAGCGGAGC AGGAAGCCTT CTACCCCGAC CACAGAGAAT
    GAGGCCAAAA TAAAGAAACC CCCACCCAAA ACACTGCCTA AACAAGGTGC TCCGCCTCAA
    ATCCAGCAGT TTCCTGATGA TATGAAGATC ATGGCAGGTC AGGAGGTGAT TCTGCAGTGT
    AAGTTCACTG GAGCACTCCC CATCAGCTGC ACCTGGCTCA AGTTCCGCAA ACCAATTCAG
    GAAGGTCAGG GCATCTCAAT AGTCAGCACA GACAGTAGCA GTCAGCTGAC CATCACAGAA
    GGACAGCAGG AGCACTGTGG CTGCTATACC ATAGAGATCA AGAACAGCTT TGGTGTGAGA
    CAGGCTGCCC TCAACCTCAC TATAGTCGAT AAGCCTGACC CCCCTGCCCG TGTCCCAGCA
    GCCTCAGATA TCCGTAAGTC CAGCCTCACG CTGTCCTGGT ATGGGCCAAC ATACGACGGT
    GGCAGCGCTG TCCAATCCTA CAACCTGGAG GCTTGGAACT CTCTGGACAT GAAGTGGACG
    GACGTGGCAT CCTGTAACAG CACATCTTAT AACGTGCAGG ACCTTCTTCC AGACAGACAG
    TACAAGTTTC GAGTGAGAGC TGTAAATATT TATGGCGTCG GGGAGCCCAG TGCCGAGTCT
    GAGCCCGTGC AGGTGGGGCT GGAGGCAGAG GGGGAAAATA AGGAGGATGA AGTCGAGCCC
    TCGGATGATG AGTCAGAGAA AGTACCAGAC TACAGAAATG TGACGATCAA AACAGACGTA
    AAAGTAAAAG ATTTTTATGA TGTAGAAGAC CGACTGGGCA CAGGGAAATT TGGGACCGTT
    TTCAAACTGA TCGAGAAGTC CACTAAAAAG GTTTGGGCTG GGAAATTCAT AAAAGCATAT
    TCTGCCAAAG AAAAAGAAAA CGTCAGGCAG GAAATTGCAA TCATGAATGA CCTGCATCAT
    CCAAAGCTGG TGCAGTGCAT CGATGCTTTT GAGGGAAAGA CCGACATCGT AATGGTGCTG
    GAAATGATCT CTGGAGGGGA ACTGTTTGAC CGGATCATAG ATGAGGACTT TGAGCTGACG
    GAGAGAGAGG TGATCAAATA TATGCTTCAG ATTGTGGACG GGGTCAATTT CATCCATAAG
    AAAGGCATCG TCCACCTGGA CCTCAAACCT GAGAACATCA TGTGTGTTAA TAAGACGGGC
    AGCAAAATCA AACTCATCGA CTTTGGACTG GCACGCAGAC TTGAGAATGC TGGATCTCTC
    AAAGTCCTGT TTGGAACCCC TGAGTTTGTG GCTCCAGAGG TGATCAATTA TGAGGCCATC
    AGTTATGCGA CAGACATGTG GAGTATAGGA GTGATCTGCT ACATCCTTGT CAGTGGTTTG
    TCTCCGTTCA TGGGGGACAA TGATAACGAG ACGCTGTCAA ATGTGACCTC AGCGACGTGG
    GACTTTGAAG ACGAGGCATT TGATGAGATC TCAGAAGAGG CCAAAGACTT TATCAGTAAT
    CTGCTAAAGA AAGACATGAA AGCTCGCCTG ACCTGTGACC AGTGTTTCCA GCATCCATGG
    CTTAAACAAG ACACCACCAA CATGGAGGCC AAGAAACTTT CCAAAGAGAG GATGAAGAAG
    TATATTCTGA GAAGAAAGTG GCAGAAAACA GGGCATGCGG TACGAGCTAT TGGTCGACTG
    TCTAACATGG CATTGATGGC AGGTGTTCAT GCCAAAAAAA GTTCCCCCAC AGAAGATGCT
    AAACAGTTTG CTGCTGAATT GGAGGCAAAA ACTTTGATGA AGCCCACGTT CAGCTCTGTT
    ATCAAAGATG TGGAGGTGGT GGAGGGAAGC GCTGCTCGAT TTGACTGCAA AATTGAAGGT
    TATCCTGACC CAGAGGTGGT GTGGTATAAG GACGATCAGC CCATTAAAGA GACGCGCCAT
    TTTCAGATAG ATTATGACGA GGACGGAAAC TGTAGTCTGG TCATTTCTGA GGTCAACGGC
    GATGATGACG CAAAATATAC CTGCAAGGCC ATGAACAGCC TCGGGGAGAC CACCTGCACC
    GCTGAACTCA TCGTAGAGGT GATGGGAGAA GAAGAAGAGG AGGAAGAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302721.1
    CDS36..5606
    Translation

    Target ORF information:

    RefSeq Version XM_009304446.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio myosin, light chain kinase a (mylka), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304446.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGAGCAGCT CTCAGCCTGT GATGTTAGGA TTCATGCCAG CAAAGTTTGG TGCCGACAGG 
    GAAAACACGT TAAACGCAGA GTGGAGTCGG GACAGCGGGG TCTGGACGCG CTCTCGACAC
    TGCACTCCGG TGAATGACAC TAATCTGCAC TGCTGTCTAC ATGGAATGAT GGCCGATGTT
    CAGTTTGTTA CAGGATCTCA TGTCTCCAAG ACAGCTCTGA CTCTGGGACG AGGTTCCCCC
    AGCAAAGAGC CCCCCGTTTT TACCCTCCCG CCCCGCAATA CCCGTGTCTG CCAAGGGGGA
    ACTGCGCGCC TTGAGGGGAA GGTACGTGGT TTCCCAGAGC CTCAGGTATG CTGGTTTAGG
    AATGGCAAGC TACTGATAGC AGGAGATCAT TATTCTATGG AACAGAGTGT CCGTGGAACA
    TTTAGTCTGG TGGTCCAGGA GGTAGAGGAT GCCGATGGAG GACGTTACAC CTGTGAAGCT
    GTTAACGATG CTGGGAGCTG CCAGGTTACT GTGGAGATCA TTGTGGAAGG AAACACAGGA
    AAGAAATACA GTCTTCCTTC CACAAACAGA ACAGGCAGAT CTATGGGAGC TCCCGCAGTA
    GAAAACAGAG CCAGTATCTG GGGTGAAAGC CCCCCAAAGT TTGTGGCTAA ACCTTCTCGC
    CTGTATCTCA AAGTGGGACA AACTGGCAAA TTTTCGGCCA AGATCACAGG ACGACCCCAG
    CCACTGGTGC AGTGGTACAA GGGAGAAGAG ATGATCCAGC AATCAGATCA CTATAACATG
    TTTGAGAGGT CAGGAATGCA TTTCCTGGAG ATCCGCAATG TGTTCTCTGA GGATGCTGGC
    ATGTACACCT GTCTGGTGGC CAACAGTGCT GGAAAAGCCT CTGCAGCCGC TGAACTCATT
    ATGCAAGATG GAGACACCGA GACAGTCATC ATGGCCAAAC CATCTCACAA TCCCTCCAAG
    TCATTCATGC TCAAAGACAC TTCCAACACT CTTGCCAACG AACAGAAAAC TTCCAGCACA
    TCCATCGAAA CCATAATCAC AGAAACACCC ACACCGCTGT CCACTACTAC TGTTCCCACT
    GTCCCATCCA TGAACAGAGG AGAAACAACA ATAGTAACAT CATCACTTCA CTCACAACCT
    ATCAGCAGGT CCACAGATGG AAGATCAGCA ACTGGCCAAA ATACTTCAAA CTTAGTCAAT
    CTAAGAAGTC AAAACAGTGT TAAGAATGAG ACGCATGGCA CAAAGAGAGT TTTGGGTATG
    TTCAGAGCTC CGGGACAGAT GCCACAATCA TCACAGATAG AATCCAGCAC TCGCCAACAA
    ACCAAAGAAA CCAGAATGGA TGTGCTAGAA ACTCCACCCA GTTTTGACTC GCAGCCCCAG
    AGCCAAGAGG TCCTGCAGGG TGAAAAGGTC ACTTTCAAAT GCCAAGTGTC CGGGTCTCCA
    GTGCCTGCAG TGAGCTGGCT GAAAGATGGA GCTCCTCTCA GACAGAGATC AGATGTCAGC
    ACTCGACAGG AGGCCGGCCT GCATGTTCTG TGTCTGGACA ATGCTCACAT AACTGACACG
    GGACAGTATG GCTGCACTGC TGCCAACAGA AGAGGAAACA CCGCGGCCCA CTGGAGTCTG
    ACCGTCAAGC GTGTGCGAGT GGATGCGAAG CCCCCAGTGT TCAGCTCTGC TCTGAAGGAT
    TGTTCGGTGT GTGAAGGACA GGAATTTACC CTGCAGTGTT CAGTGGAGGG CAAACCACAG
    CCTGAGCTCT CCTGGCTGCA CAACGATAAG CCCATTCCAT TCTCCCATAC CATGTTTGAC
    AACAGTAAAG CTCAGCTGAG AGTTCAGGCG GCTGTATTGG AGGATGCAGG CGTTTATTCG
    TGTGTAGCAG TGAATGAGCA TGGCAGAGCG GTGTGTACAG CCCATGTGCT GGTTAAAGCA
    AGGCCAAGGG TGAAAAGGTC TGCTGCTCCT CCTGTTACTG ATCTGAGGAG AACATCTGCT
    CCGCTGTTCC TCAAAGGTCT ACAGGACCTC AGGGTGATGG ACGGCAGTCA GGTCATCATG
    ACCGTGGAAG TGACAGGAAA CCCTGCACCT GAGGTGGTCT GGCTGCATAA TGGAAAAGAG
    ATCCAGGAGT CAGAGGACTT TCACTTTGAA AAGAAGGGCG GTCAGTATAC ACTCTTCATA
    CAGGAGGTGT TTCCTGAGGA CACGGGGACA TACACTTGTG AGGTGTGGAA TGAAGCCGGC
    AACGCTCGAA CTCAAGCTTC ACTCACTGTA CAAGAGCCTC AAGATGGTGT TCAGCCCTGG
    TTCATTACTA AAACCAAGTC CTCCACCGCC TCTCTGGGTC AACATGCTCT GCTCTCTTGC
    GCCATTGCTG GTGACCCCTT CCCAGAATTC CTCTGGATGA AGGATGGACA GGTAATATCT
    GGTGATGGGG ACTTTGAGAT CCTACAGAAG GAAGATGTTG TCTCGCTGCT TATCAGGAAA
    GTGGCGATGC ATCACGCTGG AAACTACGAG ATTCATCTTA AAAATAAAGT AGGAGAGTGC
    CGGTCAGTGG CCACTCTGCA TGTCAGCGAG GATCCCATGC TGTCTGCTGA TGATGCTAAC
    AAACGCCGAG GGGTGGAGAA GACTGCGCTA GGCAGGGGAG GACAGCCAAA GACTCCCAGC
    CCTGCAGCAT CGTTGCACTC TGAGGAACAT AGCAGTGGGA CAGATCAGCA AGAAGAGGAG
    GGGGGCTCCA CGCGTGGGCT TCTCAAACGG CGTGTAGAAA CGAAGGAGCA TCGGGAAGAT
    CAGATCCGAC AGCAGGAGGC CGAACAGATC GACTTCAGAA CCGTGCTCGG CCGCAAGGTC
    ACCACTAAGA GCGTCTCAGA GGAAGATCTG AAGGAGATCA CGGCTGAGCA GATGGACTTC
    AGAGGGAACC TCCAACGGCA GATCAAGCCC AAGACTCAGA CAGAAGAGCA ACGCAAAGTC
    AACACTCCGC AGCAGGTGGA CTTCAGAGCG GTGCTAGGGA AAAAAGCAAG CCAAGGCCCA
    AAACCTGCTC CTGGATCCTT GAAAACGCAA CCTAGCAAAA ACGAAGCCGT TGACTTCAGA
    TCTGTTTTAG GCAATAAGAA AAAGCCACCA ACCCAGGATA GGAATGGAGA AAGCCAAGGC
    GACCCCAGAG GAAAGGAAAA TGACGTGAAT TGTGTGGATG GAGAGATAAA GGAGACCACG
    AAGGCAGCTG GGAAGAAGGA GCCTGTTTTT GTAGAGAAAC TGAGTGATGT GACTGTGCTG
    GACGGAGAGC GTTTGCATCT ACAGTGCCAG CTGTCATCTG AGTCTCCTGC TAAAGTGACA
    TGGATGCTTG ATGGAAAAGT CATCAGACCC TCCAAATTCA TCATTATCAC TAATGAGGGT
    GATCGGTACT CTTTATCTAT TGATAAAGCT CTGCCTGAGG ATGAAGGGCA GTACGTGTGC
    AGGGCAGAGA CCGATGAGGG CAAGACTGAG TGTTCCTGTG TGGTCCTAGT GAACGATCCC
    AGCGTCTCCA CACCAGATGA TAAGCGGAGC AGGAAGCCTT CTACCCCGAC CACAGAGAAT
    GAGGCCAAAA TAAAGAAACC CCCACCCAAA ACACTGCCTA AACAAGGTGC TCCGCCTCAA
    ATCCAGCAGT TTCCTGATGA TATGAAGATC ATGGCAGGTC AGGAGGTGAT TCTGCAGTGT
    AAGTTCACTG GAGCACTCCC CATCAGCTGC ACCTGGCTCA AGTTCCGCAA ACCAATTCAG
    GAAGGTCAGG GCATCTCAAT AGTCAGCACA GACAGTAGCA GTCAGCTGAC CATCACAGAA
    GGACAGCAGG AGCACTGTGG CTGCTATACC ATAGAGATCA AGAACAGCTT TGGTGTGAGA
    CAGGCTGCCC TCAACCTCAC TATAGTCGAT AAGCCTGACC CCCCTGCCCG TGTCCCAGCA
    GCCTCAGATA TCCGTAAGTC CAGCCTCACG CTGTCCTGGT ATGGGCCAAC ATACGACGGT
    GGCAGCGCTG TCCAATCCTA CAACCTGGAG GCTTGGAACT CTCTGGACAT GAAGTGGACG
    GACGTGGCAT CCTGTAACAG CACATCTTAT AACGTGCAGG ACCTTCTTCC AGACAGACAG
    TACAAGTTTC GAGTGAGAGC TGTAAATATT TATGGCGTCG GGGAGCCCAG TGCCGAGTCT
    GAGCCCGTGC AGGTGGGGCT GGAGGCAGAG GGGGAAAATA AGGAGGATGA AGTCGAGCCC
    TCGGATGATG AGTCAGAGAA AGTACCAGAC TACAGAAATG TGACGATCAA AACAGACGTA
    AAAGTAAAAG ATTTTTATGA TGTAGAAGAC CGACTGGGCA CAGGGAAATT TGGGACCGTT
    TTCAAACTGA TCGAGAAGTC CACTAAAAAG GTTTGGGCTG GGAAATTCAT AAAAGCATAT
    TCTGCCAAAG AAAAAGAAAA CGTCAGGCAG GAAATTGCAA TCATGAATGA CCTGCATCAT
    CCAAAGCTGG TGCAGTGCAT CGATGCTTTT GAGGGAAAGA CCGACATCGT AATGGTGCTG
    GAAATGATCT CTGGAGGGGA ACTGTTTGAC CGGATCATAG ATGAGGACTT TGAGCTGACG
    GAGAGAGAGG TGATCAAATA TATGCTTCAG ATTGTGGACG GGGTCAATTT CATCCATAAG
    AAAGGCATCG TCCACCTGGA CCTCAAACCT GAGAACATCA TGTGTGTTAA TAAGACGGGC
    AGCAAAATCA AACTCATCGA CTTTGGACTG GCACGCAGAC TTGAGAATGC TGGATCTCTC
    AAAGTCCTGT TTGGAACCCC TGAGTTTGTG GCTCCAGAGG TGATCAATTA TGAGGCCATC
    AGTTATGCGA CAGACATGTG GAGTATAGGA GTGATCTGCT ACATCCTTGT CAGTGGTTTG
    TCTCCGTTCA TGGGGGACAA TGATAACGAG ACGCTGTCAA ATGTGACCTC AGCGACGTGG
    GACTTTGAAG ACGAGGCATT TGATGAGATC TCAGAAGAGG CCAAAGACTT TATCAGTAAT
    CTGCTAAAGA AAGACATGAA AGCTCGCCTG ACCTGTGACC AGTGTTTCCA GCATCCATGG
    CTTAAACAAG ACACCACCAA CATGGAGGCC AAGAAACTTT CCAAAGAGAG GATGAAGAAG
    TATATTCTGA GAAGAAAGTG GCAGAAAACA GGGCATGCGG TACGAGCTAT TGGTCGACTG
    TCTAACATGG CATTGATGGC AGGTGTTCAT GCCAAAAAAA GTTCCCCCAC AGAAGATGCT
    AAACAGTTTG CTGCTGAATT GGAGGCAAAA ACTTTGATGA AGCCCACGTT CAGCTCTGTT
    ATCAAAGATG TGGAGGTGGT GGAGGGAAGC GCTGCTCGAT TTGACTGCAA AATTGAAGGT
    TATCCTGACC CAGAGGTGGT GTGGTATAAG GACGATCAGC CCATTAAAGA GACGCGCCAT
    TTTCAGATAG ATTATGACGA GGACGGAAAC TGTAGTCTGG TCATTTCTGA GGTCAACGGC
    GATGATGACG CAAAATATAC CTGCAAGGCC ATGAACAGCC TCGGGGAGAC CACCTGCACC
    GCTGAACTCA TCGTAGAGGT GATGGGAGAA GAAGAAGAGG AGGAAGAGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.