arhgap32b gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_021469079.1	XP_021324754.1	rho GTPase-activating protein 32 isoform X2
XM_005161263.3	XP_005161320.1	rho GTPase-activating protein 32 isoform X4
XM_009295413.2	XP_009293688.1	rho GTPase-activating protein 32 isoform X2
XM_009295415.2	XP_009293690.1	rho GTPase-activating protein 32 isoform X3
XM_009295410.3	XP_009293685.1	rho GTPase-activating protein 32 isoform X1
XM_009295412.3	XP_009293687.1	rho GTPase-activating protein 32 isoform X1
XM_009295413.3	XP_009293688.1	rho GTPase-activating protein 32 isoform X2
XM_021469080.1	XP_021324755.1	rho GTPase-activating protein 32 isoform X3
XM_009295417.3	XP_009293692.1	rho GTPase-activating protein 32 isoform X6
XM_009295411.2	XP_009293686.1	rho GTPase-activating protein 32 isoform X1
XM_009295412.2	XP_009293687.1	rho GTPase-activating protein 32 isoform X1
XM_009295415.3	XP_009293690.1	rho GTPase-activating protein 32 isoform X4
XM_005161263.4	XP_005161320.1	rho GTPase-activating protein 32 isoform X5
XM_021469081.1	XP_021324756.1	rho GTPase-activating protein 32 isoform X5
XM_009295410.2	XP_009293685.1	rho GTPase-activating protein 32 isoform X1
XM_009295417.2	XP_009293692.1	rho GTPase-activating protein 32 isoform X5
XM_009295411.3	XP_009293686.1	rho GTPase-activating protein 32 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa45743	XM_009295417.3 Latest version!	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa45741	XM_021469080.1 Latest version!	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa25889	XM_005161263.4 Latest version!	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa25889	XM_021469081.1 Latest version!	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa45739	XM_009295412.3 Latest version!	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa45739	XM_009295411.3 Latest version!	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa45739	XM_009295410.3 Latest version!	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa45740	XM_009295413.3 Latest version!	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa45740	XM_021469079.1 Latest version!	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa45742	XM_009295415.3 Latest version!	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa45740	XM_009295413.2	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa45739	XM_009295412.2	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa45742	XM_009295415.2	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa25889	XM_005161263.3	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa45739	XM_009295410.2	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa45743	XM_009295417.2	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa45739	XM_009295411.2	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

arhgap32b ( XM_009295417.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293692.1 Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X10, mRNA.

Price & Availability↑

CloneID	ODa45743
Clone ID Related Accession (Same CDS sequence)	XM_009295417.3 , XM_009295417.2
Accession Version	XM_009295417.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho GTPase-activating protein 32 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295417.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGT CTCTTTTCTC CCCTCAGGGG CCTGAGGCCT 
GGACGGCACC CTGAAGAAGA CGATATCGTG CCAGAGCTTG CCAGGAGCAT CCACCCCCGA 
GAGCGACCTG ACTGGGAGGA AACCATCAGC GCAATGGCAC GAAGTGCTGA AATTCCAGAG 
CTGAGCTCTG AGCCGCTCAT GCGCTCCTGC AGCAGCACCG CCAGTATGAA GGTGAAGAAT 
GTCAAAAAGC TGTCATTCAC CAAGGGCCAT TTTCCAAAGT TAGCTGAATG TGCCCACTTC 
CACTATGAAA ACGTTGATTT TGGAACAATA CAGTTAACCC TCGCAGATGA GCAGACTGAA 
GTGACACGGA ATGGCCTGGA GTCAAAAGAG CCTGTGTATT TGGTTCAGAT CTACTGTCAG 
GGACGAAGCT GGATCGTGAG ACGAAGCTAT GAAGATTTCC GAGTCTTGGA TAAGCATCTC 
CATCTCTGCA TCTATGACCG CCGATTTTCC CAGCTCCCTG AGCTGCCCCG TTTCGACAGC 
CTGAGGGAGA GAGCGGAGGC AGTTTCCCAG ATGCTGATGG CTTACCTCTC CCGTCTCTCA 
GCCATTGCTG ATAACAAGAT CAATTGTGGG CCGGCCCTCA CGTGGATGGA GGTTGACAAC 
AAAGGGAATC ATTTGCTGGT GCATGAAGAA TCATCTATAA ATGTTCCAGC TATCGCAGCG 
GCCCACGTGA TCAAGCGCTA CATCGCACAG GCAGCTGATG AGCTGTCATT TGAGGTGGGG 
GATATTGTTT CTGTCATTGA CATGCCACCC AAAGAGGACA CCACCTGGTG GAGGGGAAAA 
CATGGTTTCC AGGTTGGCTT CTTTCCCAGC GAGTGTGTGG AGCTGATAAA CGACAAGGTC 
CCGCAGTCTG TCACCAACTC TGTGCCAAAA CCAGCGTCCC CCTGCTCTGG TCTGCGGCCG 
GCATCCTGGA GTCTGTTCCC ACCCTATTTG GAGATGGAGA GTATAAAGCA GGACAGTGCA 
TTGGCACCCG ACCCATTAAA CCCCTACAGG CTGAGCTCAG TATCCAAGAA ACACGGGAAA 
CTGATCACTT TTCTGCGCAC ATTCATGAAG TCCAGGCCGA CCAAGCAGAA GCTGAAGCAA 
AGAGGTATCC TCAGGGAGAG GGTGTTCGGC TGTGACCTAG GAGAGCATCT CCTCAACTCT 
GGCCATGATG TCCCCCAGGT ACTCAAGAGC TGCACCGAGT TCATTGAGAA GCATGGAGTG 
GTTGATGGAA TGTATCGTCT CTCTGGGATC GCCTCCAACA TTCAGAAGCT GCGGCACGAG 
TTTGATTCTG AACAGATCCC AGATCTGACA AAAGATGTTT ACATTCAGGA TATACACTGC 
GTGGGCTCCC TGTGCAAGCT CTACTTTCGA GAGCTTCCCA ACCCACTTCT CACCTACCAG 
CTGTACGAGA AATTCTCGGA GGCTGTGTCA GCAGCCACAG ACGAGGAGAG GCTGATCAAA 
ATACATGACG TCATTCAGCA GCTGCCGCCT CCGCATTATA GAACCCTTGA GTTTCTTATG 
AGGCATCTTT CACACTTGGC AACCTTCAGC TATGTAACGA ACATGCATAC TAAAAATTTG 
GCCATCGTTT GGGCCCCCAA CTTACTGAGG TCAAAGCAAA TAGAATCTGC TTGCTTCAGT 
GGGACAGCAG CCTTCATGGA AGTACGAATA CAATCGGTTG TGGTGGAGTT CATCCTGAAT 
CATGTGGATG TTCTCTTCAG TCCCAAACTG AGCTCTCTTA TTCGAGAGGG AACAGGACAC 
AACTCTTTGT CTCGGCCTAA GTCTTTGCTA GTACCGTCTC CATCCACAAA GCTGCTAACT 
CTAGAAGAGG CACAGGCACG CACACAGGCC CAGATCAACT CACCCATCAC TGCAGACAGC 
AAGTACATAG AGGTCGGAGA GGGTCCTGCA GCATTACAAG GAAAATTCCA CACAGTCATA 
GAATTCCCTA CTGAGAGGAA GAGGCAGCCC ATGAAGTCTA AGAAGTCTCC AGTTGGCAGT 
TGGCGTTCTT TTTTCAACCT GGGCAAGTCT TCTTCAATGT CGAAACGCAA ACTACAGCGT 
AATCCCAGTG AACCAAATGA ACTCAAGGCT ATGGCACTGG CTGGAGGAAG AGGAGACACT 
GGTACTCTTC GGTCAGCCAA AAGTGAAGAG TCTCTCACCT CTCTACATAA TGTTGAAGGA 
GAGTCTAAAG TGTATAGGCC CCGCAGACCT CGCTCCAGCA GTGATGCCCT CTCTGCTTCA 
TTCAATGGTG ACCTTCTGGA TACCAGACAG CACTGTAACT CGTACGACAA CCTAGGTCAT 
GGAGACAGTG ATGGGGATGA TGGTCCAATC TGTGTGCCTG CTCTCATCTC CCCACCACGC 
TCCGCTGATG ATGTGGATCT GAGCCCCCCT GACATTGGCA TGGCCTCTTT AGACTTTGAC 
CCCATGTCTT TCCAATGTAG CCTTCCTCTG GCAGAAACTT CGATTTTCTC AATTGACGCA 
GATACAAGCA GTCTAAAAAG GAGCCCTGCC AGTGGCTCAG AGTCAGTCTC GCCAGTCCGA 
GCTAAAGTTA CGAGCCGGTT TTTGTCTCCA GACCACAGTA CAGCCTTAAA GGATAAACAT 
AAACTGCTCC TTACATCTTT CTCTGAGAAA ACTCCAGTTC AGTCTGTTGA AAGCACCGAG 
ATGTCAGCAG TTGTTTCTCC TCCAAGCATC TCTGAATCAG CTGCCTCCGT GATAATGAAA 
GGACCAGAGA GCACTGTGCT TCAGCCCTCT TCCCCTCCAT CTCCACTCGA CTCTCCTACA 
AAATTGAGCT CTCTGTCAAG AACCACCGAT CTGCCCCTGA GTGAAGCCAT TCAACAAGAG 
CTTCTTTCTA AAGCTGCTAC CTTTGAGAGT AAAGACATAT CGAGCATTGA ATGTTCACAA 
CCACCACAGG TCACCTGCTC TCAAGAACAG CCAGGTTTAG TTCACCAGGC TTTTACCTGT 
AATGCTTTGC ACGAGTGCCC TCAATGTAGA GAAATCTTTT CACGATCTGT AGAAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293692.1
CDS	524..3643
Translation	MEAGSGAAAVVGSAALGLLGATGSHDISDSLFSPLRGLRPGRHPEEDDIVPELARSIHPRERPDWEETISAMARSAEIPELSSEPLMRSCSSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGTIQLTLADEQTEVTRNGLESKEPVYLVQIYCQGRSWIVRRSYEDFRVLDKHLHLCIYDRRFSQLPELPRFDSLRERAEAVSQMLMAYLSRLSAIADNKINCGPALTWMEVDNKGNHLLVHEESSINVPAIAAAHVIKRYIAQAADELSFEVGDIVSVIDMPPKEDTTWWRGKHGFQVGFFPSECVELINDKVPQSVTNSVPKPASPCSGLRPASWSLFPPYLEMESIKQDSALAPDPLNPYRLSSVSKKHGKLITFLRTFMKSRPTKQKLKQRGILRERVFGCDLGEHLLNSGHDVPQVLKSCTEFIEKHGVVDGMYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSEAVSAATDEERLIKIHDVIQQLPPPHYRTLEFLMRHLSHLATFSYVTNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVDVLFSPKLSSLIREGTGHNSLSRPKSLLVPSPSTKLLTLEEAQARTQAQINSPITADSKYIEVGEGPAALQGKFHTVIEFPTERKRQPMKSKKSPVGSWRSFFNLGKSSSMSKRKLQRNPSEPNELKAMALAGGRGDTGTLRSAKSEESLTSLHNVEGESKVYRPRRPRSSSDALSASFNGDLLDTRQHCNSYDNLGHGDSDGDDGPICVPALISPPRSADDVDLSPPDIGMASLDFDPMSFQCSLPLAETSIFSIDADTSSLKRSPASGSESVSPVRAKVTSRFLSPDHSTALKDKHKLLLTSFSEKTPVQSVESTEMSAVVSPPSISESAASVIMKGPESTVLQPSSPPSPLDSPTKLSSLSRTTDLPLSEAIQQELLSKAATFESKDISSIECSQPPQVTCSQEQPGLVHQAFTCNALHECPQCREIFSRSVEI

Target ORF information:

RefSeq Version	XM_009295417.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295417.3

ORF Insert Sequence:

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGT CTCTTTTCTC CCCTCAGGGG CCTGAGGCCT 
GGACGGCACC CTGAAGAAGA CGATATCGTG CCAGAGCTTG CCAGGAGCAT CCACCCCCGA 
GAGCGACCTG ACTGGGAGGA AACCATCAGC GCAATGGCAC GAAGTGCTGA AATTCCAGAG 
CTGAGCTCTG AGCCGCTCAT GCGCTCCTGC AGCAGCACCG CCAGTATGAA GGTGAAGAAT 
GTCAAAAAGC TGTCATTCAC CAAGGGCCAT TTTCCAAAGT TAGCTGAATG TGCCCACTTC 
CACTATGAAA ACGTTGATTT TGGAACAATA CAGTTAACCC TCGCAGATGA GCAGACTGAA 
GTGACACGGA ATGGCCTGGA GTCAAAAGAG CCTGTGTATT TGGTTCAGAT CTACTGTCAG 
GGACGAAGCT GGATCGTGAG ACGAAGCTAT GAAGATTTCC GAGTCTTGGA TAAGCATCTC 
CATCTCTGCA TCTATGACCG CCGATTTTCC CAGCTCCCTG AGCTGCCCCG TTTCGACAGC 
CTGAGGGAGA GAGCGGAGGC AGTTTCCCAG ATGCTGATGG CTTACCTCTC CCGTCTCTCA 
GCCATTGCTG ATAACAAGAT CAATTGTGGG CCGGCCCTCA CGTGGATGGA GGTTGACAAC 
AAAGGGAATC ATTTGCTGGT GCATGAAGAA TCATCTATAA ATGTTCCAGC TATCGCAGCG 
GCCCACGTGA TCAAGCGCTA CATCGCACAG GCAGCTGATG AGCTGTCATT TGAGGTGGGG 
GATATTGTTT CTGTCATTGA CATGCCACCC AAAGAGGACA CCACCTGGTG GAGGGGAAAA 
CATGGTTTCC AGGTTGGCTT CTTTCCCAGC GAGTGTGTGG AGCTGATAAA CGACAAGGTC 
CCGCAGTCTG TCACCAACTC TGTGCCAAAA CCAGCGTCCC CCTGCTCTGG TCTGCGGCCG 
GCATCCTGGA GTCTGTTCCC ACCCTATTTG GAGATGGAGA GTATAAAGCA GGACAGTGCA 
TTGGCACCCG ACCCATTAAA CCCCTACAGG CTGAGCTCAG TATCCAAGAA ACACGGGAAA 
CTGATCACTT TTCTGCGCAC ATTCATGAAG TCCAGGCCGA CCAAGCAGAA GCTGAAGCAA 
AGAGGTATCC TCAGGGAGAG GGTGTTCGGC TGTGACCTAG GAGAGCATCT CCTCAACTCT 
GGCCATGATG TCCCCCAGGT ACTCAAGAGC TGCACCGAGT TCATTGAGAA GCATGGAGTG 
GTTGATGGAA TGTATCGTCT CTCTGGGATC GCCTCCAACA TTCAGAAGCT GCGGCACGAG 
TTTGATTCTG AACAGATCCC AGATCTGACA AAAGATGTTT ACATTCAGGA TATACACTGC 
GTGGGCTCCC TGTGCAAGCT CTACTTTCGA GAGCTTCCCA ACCCACTTCT CACCTACCAG 
CTGTACGAGA AATTCTCGGA GGCTGTGTCA GCAGCCACAG ACGAGGAGAG GCTGATCAAA 
ATACATGACG TCATTCAGCA GCTGCCGCCT CCGCATTATA GAACCCTTGA GTTTCTTATG 
AGGCATCTTT CACACTTGGC AACCTTCAGC TATGTAACGA ACATGCATAC TAAAAATTTG 
GCCATCGTTT GGGCCCCCAA CTTACTGAGG TCAAAGCAAA TAGAATCTGC TTGCTTCAGT 
GGGACAGCAG CCTTCATGGA AGTACGAATA CAATCGGTTG TGGTGGAGTT CATCCTGAAT 
CATGTGGATG TTCTCTTCAG TCCCAAACTG AGCTCTCTTA TTCGAGAGGG AACAGGACAC 
AACTCTTTGT CTCGGCCTAA GTCTTTGCTA GTACCGTCTC CATCCACAAA GCTGCTAACT 
CTAGAAGAGG CACAGGCACG CACACAGGCC CAGATCAACT CACCCATCAC TGCAGACAGC 
AAGTACATAG AGGTCGGAGA GGGTCCTGCA GCATTACAAG GAAAATTCCA CACAGTCATA 
GAATTCCCTA CTGAGAGGAA GAGGCAGCCC ATGAAGTCTA AGAAGTCTCC AGTTGGCAGT 
TGGCGTTCTT TTTTCAACCT GGGCAAGTCT TCTTCAATGT CGAAACGCAA ACTACAGCGT 
AATCCCAGTG AACCAAATGA ACTCAAGGCT ATGGCACTGG CTGGAGGAAG AGGAGACACT 
GGTACTCTTC GGTCAGCCAA AAGTGAAGAG TCTCTCACCT CTCTACATAA TGTTGAAGGA 
GAGTCTAAAG TGTATAGGCC CCGCAGACCT CGCTCCAGCA GTGATGCCCT CTCTGCTTCA 
TTCAATGGTG ACCTTCTGGA TACCAGACAG CACTGTAACT CGTACGACAA CCTAGGTCAT 
GGAGACAGTG ATGGGGATGA TGGTCCAATC TGTGTGCCTG CTCTCATCTC CCCACCACGC 
TCCGCTGATG ATGTGGATCT GAGCCCCCCT GACATTGGCA TGGCCTCTTT AGACTTTGAC 
CCCATGTCTT TCCAATGTAG CCTTCCTCTG GCAGAAACTT CGATTTTCTC AATTGACGCA 
GATACAAGCA GTCTAAAAAG GAGCCCTGCC AGTGGCTCAG AGTCAGTCTC GCCAGTCCGA 
GCTAAAGTTA CGAGCCGGTT TTTGTCTCCA GACCACAGTA CAGCCTTAAA GGATAAACAT 
AAACTGCTCC TTACATCTTT CTCTGAGAAA ACTCCAGTTC AGTCTGTTGA AAGCACCGAG 
ATGTCAGCAG TTGTTTCTCC TCCAAGCATC TCTGAATCAG CTGCCTCCGT GATAATGAAA 
GGACCAGAGA GCACTGTGCT TCAGCCCTCT TCCCCTCCAT CTCCACTCGA CTCTCCTACA 
AAATTGAGCT CTCTGTCAAG AACCACCGAT CTGCCCCTGA GTGAAGCCAT TCAACAAGAG 
CTTCTTTCTA AAGCTGCTAC CTTTGAGAGT AAAGACATAT CGAGCATTGA ATGTTCACAA 
CCACCACAGG TCACCTGCTC TCAAGAACAG CCAGGTTTAG TTCACCAGGC TTTTACCTGT 
AATGCTTTGC ACGAGTGCCC TCAATGTAGA GAAATCTTTT CACGATCTGT AGAAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32b ( XM_009295417.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293692.1 Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa45743
Clone ID Related Accession (Same CDS sequence)	XM_009295417.3 , XM_009295417.2
Accession Version	XM_009295417.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3120bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho GTPase-activating protein 32 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295417.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGT CTCTTTTCTC CCCTCAGGGG CCTGAGGCCT 
GGACGGCACC CTGAAGAAGA CGATATCGTG CCAGAGCTTG CCAGGAGCAT CCACCCCCGA 
GAGCGACCTG ACTGGGAGGA AACCATCAGC GCAATGGCAC GAAGTGCTGA AATTCCAGAG 
CTGAGCTCTG AGCCGCTCAT GCGCTCCTGC AGCAGCACCG CCAGTATGAA GGTGAAGAAT 
GTCAAAAAGC TGTCATTCAC CAAGGGCCAT TTTCCAAAGT TAGCTGAATG TGCCCACTTC 
CACTATGAAA ACGTTGATTT TGGAACAATA CAGTTAACCC TCGCAGATGA GCAGACTGAA 
GTGACACGGA ATGGCCTGGA GTCAAAAGAG CCTGTGTATT TGGTTCAGAT CTACTGTCAG 
GGACGAAGCT GGATCGTGAG ACGAAGCTAT GAAGATTTCC GAGTCTTGGA TAAGCATCTC 
CATCTCTGCA TCTATGACCG CCGATTTTCC CAGCTCCCTG AGCTGCCCCG TTTCGACAGC 
CTGAGGGAGA GAGCGGAGGC AGTTTCCCAG ATGCTGATGG CTTACCTCTC CCGTCTCTCA 
GCCATTGCTG ATAACAAGAT CAATTGTGGG CCGGCCCTCA CGTGGATGGA GGTTGACAAC 
AAAGGGAATC ATTTGCTGGT GCATGAAGAA TCATCTATAA ATGTTCCAGC TATCGCAGCG 
GCCCACGTGA TCAAGCGCTA CATCGCACAG GCAGCTGATG AGCTGTCATT TGAGGTGGGG 
GATATTGTTT CTGTCATTGA CATGCCACCC AAAGAGGACA CCACCTGGTG GAGGGGAAAA 
CATGGTTTCC AGGTTGGCTT CTTTCCCAGC GAGTGTGTGG AGCTGATAAA CGACAAGGTC 
CCGCAGTCTG TCACCAACTC TGTGCCAAAA CCAGCGTCCC CCTGCTCTGG TCTGCGGCCG 
GCATCCTGGA GTCTGTTCCC ACCCTATTTG GAGATGGAGA GTATAAAGCA GGACAGTGCA 
TTGGCACCCG ACCCATTAAA CCCCTACAGG CTGAGCTCAG TATCCAAGAA ACACGGGAAA 
CTGATCACTT TTCTGCGCAC ATTCATGAAG TCCAGGCCGA CCAAGCAGAA GCTGAAGCAA 
AGAGGTATCC TCAGGGAGAG GGTGTTCGGC TGTGACCTAG GAGAGCATCT CCTCAACTCT 
GGCCATGATG TCCCCCAGGT ACTCAAGAGC TGCACCGAGT TCATTGAGAA GCATGGAGTG 
GTTGATGGAA TGTATCGTCT CTCTGGGATC GCCTCCAACA TTCAGAAGCT GCGGCACGAG 
TTTGATTCTG AACAGATCCC AGATCTGACA AAAGATGTTT ACATTCAGGA TATACACTGC 
GTGGGCTCCC TGTGCAAGCT CTACTTTCGA GAGCTTCCCA ACCCACTTCT CACCTACCAG 
CTGTACGAGA AATTCTCGGA GGCTGTGTCA GCAGCCACAG ACGAGGAGAG GCTGATCAAA 
ATACATGACG TCATTCAGCA GCTGCCGCCT CCGCATTATA GAACCCTTGA GTTTCTTATG 
AGGCATCTTT CACACTTGGC AACCTTCAGC TATGTAACGA ACATGCATAC TAAAAATTTG 
GCCATCGTTT GGGCCCCCAA CTTACTGAGG TCAAAGCAAA TAGAATCTGC TTGCTTCAGT 
GGGACAGCAG CCTTCATGGA AGTACGAATA CAATCGGTTG TGGTGGAGTT CATCCTGAAT 
CATGTGGATG TTCTCTTCAG TCCCAAACTG AGCTCTCTTA TTCGAGAGGG AACAGGACAC 
AACTCTTTGT CTCGGCCTAA GTCTTTGCTA GTACCGTCTC CATCCACAAA GCTGCTAACT 
CTAGAAGAGG CACAGGCACG CACACAGGCC CAGATCAACT CACCCATCAC TGCAGACAGC 
AAGTACATAG AGGTCGGAGA GGGTCCTGCA GCATTACAAG GAAAATTCCA CACAGTCATA 
GAATTCCCTA CTGAGAGGAA GAGGCAGCCC ATGAAGTCTA AGAAGTCTCC AGTTGGCAGT 
TGGCGTTCTT TTTTCAACCT GGGCAAGTCT TCTTCAATGT CGAAACGCAA ACTACAGCGT 
AATCCCAGTG AACCAAATGA ACTCAAGGCT ATGGCACTGG CTGGAGGAAG AGGAGACACT 
GGTACTCTTC GGTCAGCCAA AAGTGAAGAG TCTCTCACCT CTCTACATAA TGTTGAAGGA 
GAGTCTAAAG TGTATAGGCC CCGCAGACCT CGCTCCAGCA GTGATGCCCT CTCTGCTTCA 
TTCAATGGTG ACCTTCTGGA TACCAGACAG CACTGTAACT CGTACGACAA CCTAGGTCAT 
GGAGACAGTG ATGGGGATGA TGGTCCAATC TGTGTGCCTG CTCTCATCTC CCCACCACGC 
TCCGCTGATG ATGTGGATCT GAGCCCCCCT GACATTGGCA TGGCCTCTTT AGACTTTGAC 
CCCATGTCTT TCCAATGTAG CCTTCCTCTG GCAGAAACTT CGATTTTCTC AATTGACGCA 
GATACAAGCA GTCTAAAAAG GAGCCCTGCC AGTGGCTCAG AGTCAGTCTC GCCAGTCCGA 
GCTAAAGTTA CGAGCCGGTT TTTGTCTCCA GACCACAGTA CAGCCTTAAA GGATAAACAT 
AAACTGCTCC TTACATCTTT CTCTGAGAAA ACTCCAGTTC AGTCTGTTGA AAGCACCGAG 
ATGTCAGCAG TTGTTTCTCC TCCAAGCATC TCTGAATCAG CTGCCTCCGT GATAATGAAA 
GGACCAGAGA GCACTGTGCT TCAGCCCTCT TCCCCTCCAT CTCCACTCGA CTCTCCTACA 
AAATTGAGCT CTCTGTCAAG AACCACCGAT CTGCCCCTGA GTGAAGCCAT TCAACAAGAG 
CTTCTTTCTA AAGCTGCTAC CTTTGAGAGT AAAGACATAT CGAGCATTGA ATGTTCACAA 
CCACCACAGG TCACCTGCTC TCAAGAACAG CCAGGTTTAG TTCACCAGGC TTTTACCTGT 
AATGCTTTGC ACGAGTGCCC TCAATGTAGA GAAATCTTTT CACGATCTGT AGAAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293692.1
CDS	527..3646
Translation	MEAGSGAAAVVGSAALGLLGATGSHDISDSLFSPLRGLRPGRHPEEDDIVPELARSIHPRERPDWEETISAMARSAEIPELSSEPLMRSCSSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGTIQLTLADEQTEVTRNGLESKEPVYLVQIYCQGRSWIVRRSYEDFRVLDKHLHLCIYDRRFSQLPELPRFDSLRERAEAVSQMLMAYLSRLSAIADNKINCGPALTWMEVDNKGNHLLVHEESSINVPAIAAAHVIKRYIAQAADELSFEVGDIVSVIDMPPKEDTTWWRGKHGFQVGFFPSECVELINDKVPQSVTNSVPKPASPCSGLRPASWSLFPPYLEMESIKQDSALAPDPLNPYRLSSVSKKHGKLITFLRTFMKSRPTKQKLKQRGILRERVFGCDLGEHLLNSGHDVPQVLKSCTEFIEKHGVVDGMYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSEAVSAATDEERLIKIHDVIQQLPPPHYRTLEFLMRHLSHLATFSYVTNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVDVLFSPKLSSLIREGTGHNSLSRPKSLLVPSPSTKLLTLEEAQARTQAQINSPITADSKYIEVGEGPAALQGKFHTVIEFPTERKRQPMKSKKSPVGSWRSFFNLGKSSSMSKRKLQRNPSEPNELKAMALAGGRGDTGTLRSAKSEESLTSLHNVEGESKVYRPRRPRSSSDALSASFNGDLLDTRQHCNSYDNLGHGDSDGDDGPICVPALISPPRSADDVDLSPPDIGMASLDFDPMSFQCSLPLAETSIFSIDADTSSLKRSPASGSESVSPVRAKVTSRFLSPDHSTALKDKHKLLLTSFSEKTPVQSVESTEMSAVVSPPSISESAASVIMKGPESTVLQPSSPPSPLDSPTKLSSLSRTTDLPLSEAIQQELLSKAATFESKDISSIECSQPPQVTCSQEQPGLVHQAFTCNALHECPQCREIFSRSVEI

Target ORF information:

RefSeq Version	XM_009295417.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295417.2

ORF Insert Sequence:

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGT CTCTTTTCTC CCCTCAGGGG CCTGAGGCCT 
GGACGGCACC CTGAAGAAGA CGATATCGTG CCAGAGCTTG CCAGGAGCAT CCACCCCCGA 
GAGCGACCTG ACTGGGAGGA AACCATCAGC GCAATGGCAC GAAGTGCTGA AATTCCAGAG 
CTGAGCTCTG AGCCGCTCAT GCGCTCCTGC AGCAGCACCG CCAGTATGAA GGTGAAGAAT 
GTCAAAAAGC TGTCATTCAC CAAGGGCCAT TTTCCAAAGT TAGCTGAATG TGCCCACTTC 
CACTATGAAA ACGTTGATTT TGGAACAATA CAGTTAACCC TCGCAGATGA GCAGACTGAA 
GTGACACGGA ATGGCCTGGA GTCAAAAGAG CCTGTGTATT TGGTTCAGAT CTACTGTCAG 
GGACGAAGCT GGATCGTGAG ACGAAGCTAT GAAGATTTCC GAGTCTTGGA TAAGCATCTC 
CATCTCTGCA TCTATGACCG CCGATTTTCC CAGCTCCCTG AGCTGCCCCG TTTCGACAGC 
CTGAGGGAGA GAGCGGAGGC AGTTTCCCAG ATGCTGATGG CTTACCTCTC CCGTCTCTCA 
GCCATTGCTG ATAACAAGAT CAATTGTGGG CCGGCCCTCA CGTGGATGGA GGTTGACAAC 
AAAGGGAATC ATTTGCTGGT GCATGAAGAA TCATCTATAA ATGTTCCAGC TATCGCAGCG 
GCCCACGTGA TCAAGCGCTA CATCGCACAG GCAGCTGATG AGCTGTCATT TGAGGTGGGG 
GATATTGTTT CTGTCATTGA CATGCCACCC AAAGAGGACA CCACCTGGTG GAGGGGAAAA 
CATGGTTTCC AGGTTGGCTT CTTTCCCAGC GAGTGTGTGG AGCTGATAAA CGACAAGGTC 
CCGCAGTCTG TCACCAACTC TGTGCCAAAA CCAGCGTCCC CCTGCTCTGG TCTGCGGCCG 
GCATCCTGGA GTCTGTTCCC ACCCTATTTG GAGATGGAGA GTATAAAGCA GGACAGTGCA 
TTGGCACCCG ACCCATTAAA CCCCTACAGG CTGAGCTCAG TATCCAAGAA ACACGGGAAA 
CTGATCACTT TTCTGCGCAC ATTCATGAAG TCCAGGCCGA CCAAGCAGAA GCTGAAGCAA 
AGAGGTATCC TCAGGGAGAG GGTGTTCGGC TGTGACCTAG GAGAGCATCT CCTCAACTCT 
GGCCATGATG TCCCCCAGGT ACTCAAGAGC TGCACCGAGT TCATTGAGAA GCATGGAGTG 
GTTGATGGAA TGTATCGTCT CTCTGGGATC GCCTCCAACA TTCAGAAGCT GCGGCACGAG 
TTTGATTCTG AACAGATCCC AGATCTGACA AAAGATGTTT ACATTCAGGA TATACACTGC 
GTGGGCTCCC TGTGCAAGCT CTACTTTCGA GAGCTTCCCA ACCCACTTCT CACCTACCAG 
CTGTACGAGA AATTCTCGGA GGCTGTGTCA GCAGCCACAG ACGAGGAGAG GCTGATCAAA 
ATACATGACG TCATTCAGCA GCTGCCGCCT CCGCATTATA GAACCCTTGA GTTTCTTATG 
AGGCATCTTT CACACTTGGC AACCTTCAGC TATGTAACGA ACATGCATAC TAAAAATTTG 
GCCATCGTTT GGGCCCCCAA CTTACTGAGG TCAAAGCAAA TAGAATCTGC TTGCTTCAGT 
GGGACAGCAG CCTTCATGGA AGTACGAATA CAATCGGTTG TGGTGGAGTT CATCCTGAAT 
CATGTGGATG TTCTCTTCAG TCCCAAACTG AGCTCTCTTA TTCGAGAGGG AACAGGACAC 
AACTCTTTGT CTCGGCCTAA GTCTTTGCTA GTACCGTCTC CATCCACAAA GCTGCTAACT 
CTAGAAGAGG CACAGGCACG CACACAGGCC CAGATCAACT CACCCATCAC TGCAGACAGC 
AAGTACATAG AGGTCGGAGA GGGTCCTGCA GCATTACAAG GAAAATTCCA CACAGTCATA 
GAATTCCCTA CTGAGAGGAA GAGGCAGCCC ATGAAGTCTA AGAAGTCTCC AGTTGGCAGT 
TGGCGTTCTT TTTTCAACCT GGGCAAGTCT TCTTCAATGT CGAAACGCAA ACTACAGCGT 
AATCCCAGTG AACCAAATGA ACTCAAGGCT ATGGCACTGG CTGGAGGAAG AGGAGACACT 
GGTACTCTTC GGTCAGCCAA AAGTGAAGAG TCTCTCACCT CTCTACATAA TGTTGAAGGA 
GAGTCTAAAG TGTATAGGCC CCGCAGACCT CGCTCCAGCA GTGATGCCCT CTCTGCTTCA 
TTCAATGGTG ACCTTCTGGA TACCAGACAG CACTGTAACT CGTACGACAA CCTAGGTCAT 
GGAGACAGTG ATGGGGATGA TGGTCCAATC TGTGTGCCTG CTCTCATCTC CCCACCACGC 
TCCGCTGATG ATGTGGATCT GAGCCCCCCT GACATTGGCA TGGCCTCTTT AGACTTTGAC 
CCCATGTCTT TCCAATGTAG CCTTCCTCTG GCAGAAACTT CGATTTTCTC AATTGACGCA 
GATACAAGCA GTCTAAAAAG GAGCCCTGCC AGTGGCTCAG AGTCAGTCTC GCCAGTCCGA 
GCTAAAGTTA CGAGCCGGTT TTTGTCTCCA GACCACAGTA CAGCCTTAAA GGATAAACAT 
AAACTGCTCC TTACATCTTT CTCTGAGAAA ACTCCAGTTC AGTCTGTTGA AAGCACCGAG 
ATGTCAGCAG TTGTTTCTCC TCCAAGCATC TCTGAATCAG CTGCCTCCGT GATAATGAAA 
GGACCAGAGA GCACTGTGCT TCAGCCCTCT TCCCCTCCAT CTCCACTCGA CTCTCCTACA 
AAATTGAGCT CTCTGTCAAG AACCACCGAT CTGCCCCTGA GTGAAGCCAT TCAACAAGAG 
CTTCTTTCTA AAGCTGCTAC CTTTGAGAGT AAAGACATAT CGAGCATTGA ATGTTCACAA 
CCACCACAGG TCACCTGCTC TCAAGAACAG CCAGGTTTAG TTCACCAGGC TTTTACCTGT 
AATGCTTTGC ACGAGTGCCC TCAATGTAGA GAAATCTTTT CACGATCTGT AGAAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32b ( XM_021469080.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324755.1 Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X6, mRNA.

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CloneID	ODa45741
Clone ID Related Accession (Same CDS sequence)	XM_021469080.1
Accession Version	XM_021469080.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho GTPase-activating protein 32 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGT CTCTTTTCTC CCCTCAGGGG CCTGAGGCCT 
GGACGGCACC CTGAAGAAGA CGATATCGTG CCAGAGCTTG CCAGGAGCAT CCACCCCCGA 
GAGCGACCTG ACTGGGAGGA AACCATCAGC GCAATGGCAC GAAGTGCTGA AATTCCAGAG 
CTGAGCTCTG AGCCGCTCAT GCGCTCCTGC AGCAGCACCG CCAGTATGAA GGTGAAGAAT 
GTCAAAAAGC TGTCATTCAC CAAGGGCCAT TTTCCAAAGT TAGCTGAATG TGCCCACTTC 
CACTATGAAA ACGTTGATTT TGGAACAATA CAGTTAACCC TCGCAGATGA GCAGACTGAA 
GTGACACGGA ATGGCCTGGA GTCAAAAGAG CCTGTGTATT TGGTTCAGAT CTACTGTCAG 
GGACGAAGCT GGATCGTGAG ACGAAGCTAT GAAGATTTCC GAGTCTTGGA TAAGCATCTC 
CATCTCTGCA TCTATGACCG CCGATTTTCC CAGCTCCCTG AGCTGCCCCG TTTCGACAGC 
CTGAGGGAGA GAGCGGAGGC AGTTTCCCAG ATGCTGATGG CTTACCTCTC CCGTCTCTCA 
GCCATTGCTG ATAACAAGAT CAATTGTGGG CCGGCCCTCA CGTGGATGGA GGTTGACAAC 
AAAGGGAATC ATTTGCTGGT GCATGAAGAA TCATCTATAA ATGTTCCAGC TATCGCAGCG 
GCCCACGTGA TCAAGCGCTA CATCGCACAG GCAGCTGATG AGCTGTCATT TGAGGTGGGG 
GATATTGTTT CTGTCATTGA CATGCCACCC AAAGAGGACA CCACCTGGTG GAGGGGAAAA 
CATGGTTTCC AGGTTGGCTT CTTTCCCAGC GAGTGTGTGG AGCTGATAAA CGACAAGGTC 
CCGCAGTCTG TCACCAACTC TGTGCCAAAA CCAGATTTGG AGATGGAGAG TATAAAGCAG 
GACAGTGCAT TGGCACCCGA CCCATTAAAC CCCTACAGGC TGAGCTCAGT ATCCAAGAAA 
CACGGGAAAC TGATCACTTT TCTGCGCACA TTCATGAAGT CCAGGCCGAC CAAGCAGAAG 
CTGAAGCAAA GAGGTATCCT CAGGGAGAGG GTGTTCGGCT GTGACCTAGG AGAGCATCTC 
CTCAACTCTG GCCATGATGT CCCCCAGGTA CTCAAGAGCT GCACCGAGTT CATTGAGAAG 
CATGGAGTGG TTGATGGAAT GTATCGTCTC TCTGGGATCG CCTCCAACAT TCAGAAGCTG 
CGGCACGAGT TTGATTCTGA ACAGATCCCA GATCTGACAA AAGATGTTTA CATTCAGGAT 
ATACACTGCG TGGGCTCCCT GTGCAAGCTC TACTTTCGAG AGCTTCCCAA CCCACTTCTC 
ACCTACCAGC TGTACGAGAA ATTCTCGGAG GCTGTGTCAG CAGCCACAGA CGAGGAGAGG 
CTGATCAAAA TACATGACGT CATTCAGCAG CTGCCGCCTC CGCATTATAG AACCCTTGAG 
TTTCTTATGA GGCATCTTTC ACACTTGGCA ACCTTCAGCT ATGTAACGAA CATGCATACT 
AAAAATTTGG CCATCGTTTG GGCCCCCAAC TTACTGAGGT CAAAGCAAAT AGAATCTGCT 
TGCTTCAGTG GGACAGCAGC CTTCATGGAA GTACGAATAC AATCGGTTGT GGTGGAGTTC 
ATCCTGAATC ATGTGGATGT TCTCTTCAGT CCCAAACTGA GCTCTCTTAT TCGAGAGGGA 
ACAGGACACA ACTCTTTGTC TCGGCCTAAG TCTTTGCTAG TACCGTCTCC ATCCACAAAG 
CTGCTAACTC TAGAAGAGGC ACAGGCACGC ACACAGGCCC AGATCAACTC ACCCATCACT 
GCAGACAGCA AGTACATAGA GGTCGGAGAG GGTCCTGCAG CATTACAAGG AAAATTCCAC 
ACAGTCATAG AATTCCCTAC TGAGAGGAAG AGGCAGCCCA TGAAGTCTAA GAAGTCTCCA 
GTTGGCAGTT GGCGTTCTTT TTTCAACCTG GGCAAGTCTT CTTCAATGTC GAAACGCAAA 
CTACAGCGTA ATCCCAGTGA ACCAAATGAA CTCAAGGCTA TGGCACTGGC TGGAGGAAGA 
GGAGACACTG GTACTCTTCG GTCAGCCAAA AGTGAAGAGT CTCTCACCTC TCTACATAAT 
GTTGAAGGAG AGTCTAAAGT GTATAGGCCC CGCAGACCTC GCTCCAGCAG TGATGCCCTC 
TCTGCTTCAT TCAATGGTGA CCTTCTGGAT ACCAGACAGC ACTGTAACTC GTACGACAAC 
CTAGGTCATG GAGACAGTGA TGGGGATGAT GGTCCAATCT GTGTGCCTGC TCTCATCTCC 
CCACCACGCT CCGCTGATGA TGTGGATCTG AGCCCCCCTG ACATTGGCAT GGCCTCTTTA 
GACTTTGACC CCATGTCTTT CCAATGTAGC CTTCCTCTGG CAGAAACTTC GATTTTCTCA 
ATTGACGCAG ATACAAGCAG TCTAAAAAGG AGCCCTGCCA GTGGCTCAGA GTCAGTCTCG 
CCAGTCCGAG CTAAAGTTAC GAGCCGGTTT TTGTCTCCAG ACCACAGTAC AGCCTTAAAG 
GATAAACATA AACTGCTCCT TACATCTTTC TCTGAGAAAA CTCCAGTTCA GTCTGTTGAA 
AGCACCGAGA TGTCAGCAGT TGTTTCTCCT CCAAGCATCT CTGAATCAGC TGCCTCCGTG 
ATAATGAAAG GACCAGAGAG CACTGTGCTT CAGCCCTCTT CCCCTCCATC TCCACTCGAC 
TCTCCTACAA AATTGAGCTC TCTGTCAAGA ACCACCGATC TGCCCCTGAG TGAAGCCATT 
CAACAAGAGC TTCTTTCTAA AGCTGCTACC TTTGAGAGTA AAGACATATC GAGCATTGAA 
TGTTCACAAC CACCACAGGT CACCTGCTCT CAAGAACAGC CAGGAGCCGT AGCTTTGGAC 
TCTCCAAAGG GCAATGTCCC TGTCAGCTCT GTGTCCCTCG TCCCTCCCCC TCCCCCACCA 
AAGAACGCTG CCCGCATGCT TGCACTAGCT CTAGCTGAAT CTGCCCAGCA GGCCACCATT 
CTTTCTCAGA AGCAATCCTC TGGACCCCCT ACACCAGTAT CCCCTCTTAG GCCTCAAGAG 
TTATTGGAGA CCCTGGACTG GCCGCTGCCT TCTTCCTTGC CATCACAGCC ATCCGAGGAG 
GAAACTGCAG TAGAATCATC AAATAAATCG GTTCCTTTGT CTTCACCACC CTCCACTCCA 
CCTGAAAAAA TGACTCGCTC AGTCAGTCTG CACCTCACCA CAGCTGATAG TGGGAAGCCA 
TCCAGCATTT CATGCAACAG CCAGGATGTA ATCTCACCAG AGACCCCAAG CCAGAGTGCT 
ATGCCCTCTT GTCTGACTTC TCATGTTTCT CAAACACAAA AAAGTCCAGA AAAACAGCAG 
CCTGCTGTCG GTCAGACACC TGTGAGCACA GCAAAAACCA CCACCACAAC CACCACTTCA 
ACCATCAGCA GTAAGGATGC TGTCATTCAA CAACCCACCT CTGAGATCCA TATGCCTGAA 
ACAGTCCTGC CACCAAAACC AAACAAGGTG CCAGATCAAC CAGTGGCAAT TCTTCAGCCT 
CAGCCTCAAA TGCAGACGCA ACCTCATACT GCATTTCGGT CTCAACCTCA AGTTCAGCCT 
CTTCCTCAGT CACAAGCACA GCCACAGTCT CAGCCCAGAT CACAGCCCAA TGCTAGAGTT 
GCACCAACCC TGGCAGCACC TCATGATCCT GTGGAAAAAC CATGGGAAGC CATAAAGCCT 
GTCCATCCAA AAGGAGATAG TGCTTCTCAA CAACACCACA CTCAGGGAGT GACACCACCA 
ACACCCCCTA TTCGCTCGAT TGAAAGTAAA CTAGCAGCAG CTGCACTTTG CAACACCGAG 
GCTGCAAACC CTGCCAACTT TCATGCCATG TTAGAGACAT CCATGAGGGG ATCAGTAGAG 
GAGACTCCCC CACAGTCTCA TCCGCCTCAC CGCAGATCGT CTACTCATCA GTCAGGTTAT 
CTTTATCACA CAAAAGGGGA GGCAGCCTTA CTGGACCCCA CCACAGAGGC ATATTACCAT 
CAGCGGGTAA CCCCTTCAGG TCAGGCTACA GTGGGGTACC ATTACCGGCC AGAGAGTGTT 
CCACCTCACA TTTCTTACGT GTCAAAATCA GAGCCACACA TTTCCTACAG AGGTCATGGG 
GATGGTCGAT ACAATACAAT TGGACCTAGA TCCTACCACC AGTCTATCAA ATCTCGTGGT 
TCACTTAGAA GTGAGTACAT CTCTCCAAGC TCATTACACC ACTCCCATAG CTACAGCCAA 
GCAGATGGAC CAACCGTCTA CCCAACAATT CGCAGGGTCC ACTCCTTACA TGTGCCTCCC 
ACTGCTATTC GCTCAATGCC AGTCACCAGA ACTGAGGTAC CACCAGATGA TGAGTTATTT 
TACTACCAGC GCCCAATCTA CCACTGCAAG TCTCACCAAC CATCATCTCA AGTCGACTAT 
CACGTCACAC AACTGCAGCC TTATTTTGAA AATGGAAGAG TTCAGTACCG CTACAGCCCC 
TACTCTGGCT CTCACGTTCT GGAATCCCCT TTCTATGATG TGGACCATTA TGGTACTATC 
CGCTTAAGGC ACGTCCATTC CTTCAGTGGT CGGGACAGTG CCCCTCTCCT TCGATCTGGA 
GCAAAATCTG GAGGCTACCA CTACCTTTCA CGGCATGTTA TTCCTTTAAA GGAGCATGGC 
TTCATAAGCA GGGACATGCC ACCTTACAGT GGTTCAAAAG GGAGTATTTA CTTTGCATGG 
GACCCAGAGG AGGCAGAAAG GCTGCGCATA CACTCCATCC GTAGGGAAAG CAGAGCAAGG 
CAAAAGGTTA AGGGTTCTGT TATGTCTCAA TACGACAATG TAGGACCCTA CATGCCTGTG 
GATGTTGACA TGTTACATTT GCGCAGCAAA TCAGATCCTG GAAAAACTGT ATTGGTGTCC 
TCGGAGAGCA AAGAGGCCAG ATACAATGTT GTGCCTGGAT CTCAGCATGC TCCCAGACAC 
TTGATCTCTG ATCCAGATGT CCTAATGTAT ATGGAAACAG AGAAGCACTG CCAAGGAAAT 
GGAATGGGGG AGAAAACCAC CAAGCAAGGC AGCTCTAGGA CCTACTCGTC TATACATTCA 
CATCAATCCC AGCTTCCACC ACTAAGCCGG CAGCACTTAC CAGAGGGTGG CCATCATGAC 
CCAAAGTTTG AACCAGGGGA AAAGCAACTG AGTGGTAAAC ATTGGCAGGA ACATTCGGAC 
AGCAGGAGTG CTCAAGCACG TTATGAAAGG TCTGATTTGG ATCGCCACAT ATGCAGGGTG 
AAGGCCACTA GCAGTGCCAG TGAAGATGAA CAAGCTGCTC CTGTGAAGCC GGCTCCTCCA 
CCTAAGCCCG AGAGATCCCA TAGTGTTCGA GAGCGGCACC ATTACAACCA GTCCAGTCTT 
CCTGCATATT TACCAGACAA TTCAGATAGA GACCACAGTG GAACATACTC CCATCAGTCA 
CAGGGGCAGA GACAAAGCAC CGTTACTTTG CAGTCGCATT ACGACAACCT GGATGACTAT 
CATCCAGTAC CTCAATCCCA AACCACGCTA GCAAATCGGG GTTGTTCCAG TTCCTATCAT 
AGCCCCGGTT TCTCAACTCC ACACAGTAAT CGTGCATACT CCACTGCCTT GGGACAGGGT 
GCCTTCATAC AAGCTGAGCT TTCAGTGCAA AGGCCAGAGA CTGAGATAAA TGCTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324755.1
CDS	521..6340
Translation	MEAGSGAAAVVGSAALGLLGATGSHDISDSLFSPLRGLRPGRHPEEDDIVPELARSIHPRERPDWEETISAMARSAEIPELSSEPLMRSCSSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGTIQLTLADEQTEVTRNGLESKEPVYLVQIYCQGRSWIVRRSYEDFRVLDKHLHLCIYDRRFSQLPELPRFDSLRERAEAVSQMLMAYLSRLSAIADNKINCGPALTWMEVDNKGNHLLVHEESSINVPAIAAAHVIKRYIAQAADELSFEVGDIVSVIDMPPKEDTTWWRGKHGFQVGFFPSECVELINDKVPQSVTNSVPKPDLEMESIKQDSALAPDPLNPYRLSSVSKKHGKLITFLRTFMKSRPTKQKLKQRGILRERVFGCDLGEHLLNSGHDVPQVLKSCTEFIEKHGVVDGMYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSEAVSAATDEERLIKIHDVIQQLPPPHYRTLEFLMRHLSHLATFSYVTNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVDVLFSPKLSSLIREGTGHNSLSRPKSLLVPSPSTKLLTLEEAQARTQAQINSPITADSKYIEVGEGPAALQGKFHTVIEFPTERKRQPMKSKKSPVGSWRSFFNLGKSSSMSKRKLQRNPSEPNELKAMALAGGRGDTGTLRSAKSEESLTSLHNVEGESKVYRPRRPRSSSDALSASFNGDLLDTRQHCNSYDNLGHGDSDGDDGPICVPALISPPRSADDVDLSPPDIGMASLDFDPMSFQCSLPLAETSIFSIDADTSSLKRSPASGSESVSPVRAKVTSRFLSPDHSTALKDKHKLLLTSFSEKTPVQSVESTEMSAVVSPPSISESAASVIMKGPESTVLQPSSPPSPLDSPTKLSSLSRTTDLPLSEAIQQELLSKAATFESKDISSIECSQPPQVTCSQEQPGAVALDSPKGNVPVSSVSLVPPPPPPKNAARMLALALAESAQQATILSQKQSSGPPTPVSPLRPQELLETLDWPLPSSLPSQPSEEETAVESSNKSVPLSSPPSTPPEKMTRSVSLHLTTADSGKPSSISCNSQDVISPETPSQSAMPSCLTSHVSQTQKSPEKQQPAVGQTPVSTAKTTTTTTTSTISSKDAVIQQPTSEIHMPETVLPPKPNKVPDQPVAILQPQPQMQTQPHTAFRSQPQVQPLPQSQAQPQSQPRSQPNARVAPTLAAPHDPVEKPWEAIKPVHPKGDSASQQHHTQGVTPPTPPIRSIESKLAAAALCNTEAANPANFHAMLETSMRGSVEETPPQSHPPHRRSSTHQSGYLYHTKGEAALLDPTTEAYYHQRVTPSGQATVGYHYRPESVPPHISYVSKSEPHISYRGHGDGRYNTIGPRSYHQSIKSRGSLRSEYISPSSLHHSHSYSQADGPTVYPTIRRVHSLHVPPTAIRSMPVTRTEVPPDDELFYYQRPIYHCKSHQPSSQVDYHVTQLQPYFENGRVQYRYSPYSGSHVLESPFYDVDHYGTIRLRHVHSFSGRDSAPLLRSGAKSGGYHYLSRHVIPLKEHGFISRDMPPYSGSKGSIYFAWDPEEAERLRIHSIRRESRARQKVKGSVMSQYDNVGPYMPVDVDMLHLRSKSDPGKTVLVSSESKEARYNVVPGSQHAPRHLISDPDVLMYMETEKHCQGNGMGEKTTKQGSSRTYSSIHSHQSQLPPLSRQHLPEGGHHDPKFEPGEKQLSGKHWQEHSDSRSAQARYERSDLDRHICRVKATSSASEDEQAAPVKPAPPPKPERSHSVRERHHYNQSSLPAYLPDNSDRDHSGTYSHQSQGQRQSTVTLQSHYDNLDDYHPVPQSQTTLANRGCSSSYHSPGFSTPHSNRAYSTALGQGAFIQAELSVQRPETEINAE

Target ORF information:

RefSeq Version	XM_021469080.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469080.1

ORF Insert Sequence:

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGT CTCTTTTCTC CCCTCAGGGG CCTGAGGCCT 
GGACGGCACC CTGAAGAAGA CGATATCGTG CCAGAGCTTG CCAGGAGCAT CCACCCCCGA 
GAGCGACCTG ACTGGGAGGA AACCATCAGC GCAATGGCAC GAAGTGCTGA AATTCCAGAG 
CTGAGCTCTG AGCCGCTCAT GCGCTCCTGC AGCAGCACCG CCAGTATGAA GGTGAAGAAT 
GTCAAAAAGC TGTCATTCAC CAAGGGCCAT TTTCCAAAGT TAGCTGAATG TGCCCACTTC 
CACTATGAAA ACGTTGATTT TGGAACAATA CAGTTAACCC TCGCAGATGA GCAGACTGAA 
GTGACACGGA ATGGCCTGGA GTCAAAAGAG CCTGTGTATT TGGTTCAGAT CTACTGTCAG 
GGACGAAGCT GGATCGTGAG ACGAAGCTAT GAAGATTTCC GAGTCTTGGA TAAGCATCTC 
CATCTCTGCA TCTATGACCG CCGATTTTCC CAGCTCCCTG AGCTGCCCCG TTTCGACAGC 
CTGAGGGAGA GAGCGGAGGC AGTTTCCCAG ATGCTGATGG CTTACCTCTC CCGTCTCTCA 
GCCATTGCTG ATAACAAGAT CAATTGTGGG CCGGCCCTCA CGTGGATGGA GGTTGACAAC 
AAAGGGAATC ATTTGCTGGT GCATGAAGAA TCATCTATAA ATGTTCCAGC TATCGCAGCG 
GCCCACGTGA TCAAGCGCTA CATCGCACAG GCAGCTGATG AGCTGTCATT TGAGGTGGGG 
GATATTGTTT CTGTCATTGA CATGCCACCC AAAGAGGACA CCACCTGGTG GAGGGGAAAA 
CATGGTTTCC AGGTTGGCTT CTTTCCCAGC GAGTGTGTGG AGCTGATAAA CGACAAGGTC 
CCGCAGTCTG TCACCAACTC TGTGCCAAAA CCAGATTTGG AGATGGAGAG TATAAAGCAG 
GACAGTGCAT TGGCACCCGA CCCATTAAAC CCCTACAGGC TGAGCTCAGT ATCCAAGAAA 
CACGGGAAAC TGATCACTTT TCTGCGCACA TTCATGAAGT CCAGGCCGAC CAAGCAGAAG 
CTGAAGCAAA GAGGTATCCT CAGGGAGAGG GTGTTCGGCT GTGACCTAGG AGAGCATCTC 
CTCAACTCTG GCCATGATGT CCCCCAGGTA CTCAAGAGCT GCACCGAGTT CATTGAGAAG 
CATGGAGTGG TTGATGGAAT GTATCGTCTC TCTGGGATCG CCTCCAACAT TCAGAAGCTG 
CGGCACGAGT TTGATTCTGA ACAGATCCCA GATCTGACAA AAGATGTTTA CATTCAGGAT 
ATACACTGCG TGGGCTCCCT GTGCAAGCTC TACTTTCGAG AGCTTCCCAA CCCACTTCTC 
ACCTACCAGC TGTACGAGAA ATTCTCGGAG GCTGTGTCAG CAGCCACAGA CGAGGAGAGG 
CTGATCAAAA TACATGACGT CATTCAGCAG CTGCCGCCTC CGCATTATAG AACCCTTGAG 
TTTCTTATGA GGCATCTTTC ACACTTGGCA ACCTTCAGCT ATGTAACGAA CATGCATACT 
AAAAATTTGG CCATCGTTTG GGCCCCCAAC TTACTGAGGT CAAAGCAAAT AGAATCTGCT 
TGCTTCAGTG GGACAGCAGC CTTCATGGAA GTACGAATAC AATCGGTTGT GGTGGAGTTC 
ATCCTGAATC ATGTGGATGT TCTCTTCAGT CCCAAACTGA GCTCTCTTAT TCGAGAGGGA 
ACAGGACACA ACTCTTTGTC TCGGCCTAAG TCTTTGCTAG TACCGTCTCC ATCCACAAAG 
CTGCTAACTC TAGAAGAGGC ACAGGCACGC ACACAGGCCC AGATCAACTC ACCCATCACT 
GCAGACAGCA AGTACATAGA GGTCGGAGAG GGTCCTGCAG CATTACAAGG AAAATTCCAC 
ACAGTCATAG AATTCCCTAC TGAGAGGAAG AGGCAGCCCA TGAAGTCTAA GAAGTCTCCA 
GTTGGCAGTT GGCGTTCTTT TTTCAACCTG GGCAAGTCTT CTTCAATGTC GAAACGCAAA 
CTACAGCGTA ATCCCAGTGA ACCAAATGAA CTCAAGGCTA TGGCACTGGC TGGAGGAAGA 
GGAGACACTG GTACTCTTCG GTCAGCCAAA AGTGAAGAGT CTCTCACCTC TCTACATAAT 
GTTGAAGGAG AGTCTAAAGT GTATAGGCCC CGCAGACCTC GCTCCAGCAG TGATGCCCTC 
TCTGCTTCAT TCAATGGTGA CCTTCTGGAT ACCAGACAGC ACTGTAACTC GTACGACAAC 
CTAGGTCATG GAGACAGTGA TGGGGATGAT GGTCCAATCT GTGTGCCTGC TCTCATCTCC 
CCACCACGCT CCGCTGATGA TGTGGATCTG AGCCCCCCTG ACATTGGCAT GGCCTCTTTA 
GACTTTGACC CCATGTCTTT CCAATGTAGC CTTCCTCTGG CAGAAACTTC GATTTTCTCA 
ATTGACGCAG ATACAAGCAG TCTAAAAAGG AGCCCTGCCA GTGGCTCAGA GTCAGTCTCG 
CCAGTCCGAG CTAAAGTTAC GAGCCGGTTT TTGTCTCCAG ACCACAGTAC AGCCTTAAAG 
GATAAACATA AACTGCTCCT TACATCTTTC TCTGAGAAAA CTCCAGTTCA GTCTGTTGAA 
AGCACCGAGA TGTCAGCAGT TGTTTCTCCT CCAAGCATCT CTGAATCAGC TGCCTCCGTG 
ATAATGAAAG GACCAGAGAG CACTGTGCTT CAGCCCTCTT CCCCTCCATC TCCACTCGAC 
TCTCCTACAA AATTGAGCTC TCTGTCAAGA ACCACCGATC TGCCCCTGAG TGAAGCCATT 
CAACAAGAGC TTCTTTCTAA AGCTGCTACC TTTGAGAGTA AAGACATATC GAGCATTGAA 
TGTTCACAAC CACCACAGGT CACCTGCTCT CAAGAACAGC CAGGAGCCGT AGCTTTGGAC 
TCTCCAAAGG GCAATGTCCC TGTCAGCTCT GTGTCCCTCG TCCCTCCCCC TCCCCCACCA 
AAGAACGCTG CCCGCATGCT TGCACTAGCT CTAGCTGAAT CTGCCCAGCA GGCCACCATT 
CTTTCTCAGA AGCAATCCTC TGGACCCCCT ACACCAGTAT CCCCTCTTAG GCCTCAAGAG 
TTATTGGAGA CCCTGGACTG GCCGCTGCCT TCTTCCTTGC CATCACAGCC ATCCGAGGAG 
GAAACTGCAG TAGAATCATC AAATAAATCG GTTCCTTTGT CTTCACCACC CTCCACTCCA 
CCTGAAAAAA TGACTCGCTC AGTCAGTCTG CACCTCACCA CAGCTGATAG TGGGAAGCCA 
TCCAGCATTT CATGCAACAG CCAGGATGTA ATCTCACCAG AGACCCCAAG CCAGAGTGCT 
ATGCCCTCTT GTCTGACTTC TCATGTTTCT CAAACACAAA AAAGTCCAGA AAAACAGCAG 
CCTGCTGTCG GTCAGACACC TGTGAGCACA GCAAAAACCA CCACCACAAC CACCACTTCA 
ACCATCAGCA GTAAGGATGC TGTCATTCAA CAACCCACCT CTGAGATCCA TATGCCTGAA 
ACAGTCCTGC CACCAAAACC AAACAAGGTG CCAGATCAAC CAGTGGCAAT TCTTCAGCCT 
CAGCCTCAAA TGCAGACGCA ACCTCATACT GCATTTCGGT CTCAACCTCA AGTTCAGCCT 
CTTCCTCAGT CACAAGCACA GCCACAGTCT CAGCCCAGAT CACAGCCCAA TGCTAGAGTT 
GCACCAACCC TGGCAGCACC TCATGATCCT GTGGAAAAAC CATGGGAAGC CATAAAGCCT 
GTCCATCCAA AAGGAGATAG TGCTTCTCAA CAACACCACA CTCAGGGAGT GACACCACCA 
ACACCCCCTA TTCGCTCGAT TGAAAGTAAA CTAGCAGCAG CTGCACTTTG CAACACCGAG 
GCTGCAAACC CTGCCAACTT TCATGCCATG TTAGAGACAT CCATGAGGGG ATCAGTAGAG 
GAGACTCCCC CACAGTCTCA TCCGCCTCAC CGCAGATCGT CTACTCATCA GTCAGGTTAT 
CTTTATCACA CAAAAGGGGA GGCAGCCTTA CTGGACCCCA CCACAGAGGC ATATTACCAT 
CAGCGGGTAA CCCCTTCAGG TCAGGCTACA GTGGGGTACC ATTACCGGCC AGAGAGTGTT 
CCACCTCACA TTTCTTACGT GTCAAAATCA GAGCCACACA TTTCCTACAG AGGTCATGGG 
GATGGTCGAT ACAATACAAT TGGACCTAGA TCCTACCACC AGTCTATCAA ATCTCGTGGT 
TCACTTAGAA GTGAGTACAT CTCTCCAAGC TCATTACACC ACTCCCATAG CTACAGCCAA 
GCAGATGGAC CAACCGTCTA CCCAACAATT CGCAGGGTCC ACTCCTTACA TGTGCCTCCC 
ACTGCTATTC GCTCAATGCC AGTCACCAGA ACTGAGGTAC CACCAGATGA TGAGTTATTT 
TACTACCAGC GCCCAATCTA CCACTGCAAG TCTCACCAAC CATCATCTCA AGTCGACTAT 
CACGTCACAC AACTGCAGCC TTATTTTGAA AATGGAAGAG TTCAGTACCG CTACAGCCCC 
TACTCTGGCT CTCACGTTCT GGAATCCCCT TTCTATGATG TGGACCATTA TGGTACTATC 
CGCTTAAGGC ACGTCCATTC CTTCAGTGGT CGGGACAGTG CCCCTCTCCT TCGATCTGGA 
GCAAAATCTG GAGGCTACCA CTACCTTTCA CGGCATGTTA TTCCTTTAAA GGAGCATGGC 
TTCATAAGCA GGGACATGCC ACCTTACAGT GGTTCAAAAG GGAGTATTTA CTTTGCATGG 
GACCCAGAGG AGGCAGAAAG GCTGCGCATA CACTCCATCC GTAGGGAAAG CAGAGCAAGG 
CAAAAGGTTA AGGGTTCTGT TATGTCTCAA TACGACAATG TAGGACCCTA CATGCCTGTG 
GATGTTGACA TGTTACATTT GCGCAGCAAA TCAGATCCTG GAAAAACTGT ATTGGTGTCC 
TCGGAGAGCA AAGAGGCCAG ATACAATGTT GTGCCTGGAT CTCAGCATGC TCCCAGACAC 
TTGATCTCTG ATCCAGATGT CCTAATGTAT ATGGAAACAG AGAAGCACTG CCAAGGAAAT 
GGAATGGGGG AGAAAACCAC CAAGCAAGGC AGCTCTAGGA CCTACTCGTC TATACATTCA 
CATCAATCCC AGCTTCCACC ACTAAGCCGG CAGCACTTAC CAGAGGGTGG CCATCATGAC 
CCAAAGTTTG AACCAGGGGA AAAGCAACTG AGTGGTAAAC ATTGGCAGGA ACATTCGGAC 
AGCAGGAGTG CTCAAGCACG TTATGAAAGG TCTGATTTGG ATCGCCACAT ATGCAGGGTG 
AAGGCCACTA GCAGTGCCAG TGAAGATGAA CAAGCTGCTC CTGTGAAGCC GGCTCCTCCA 
CCTAAGCCCG AGAGATCCCA TAGTGTTCGA GAGCGGCACC ATTACAACCA GTCCAGTCTT 
CCTGCATATT TACCAGACAA TTCAGATAGA GACCACAGTG GAACATACTC CCATCAGTCA 
CAGGGGCAGA GACAAAGCAC CGTTACTTTG CAGTCGCATT ACGACAACCT GGATGACTAT 
CATCCAGTAC CTCAATCCCA AACCACGCTA GCAAATCGGG GTTGTTCCAG TTCCTATCAT 
AGCCCCGGTT TCTCAACTCC ACACAGTAAT CGTGCATACT CCACTGCCTT GGGACAGGGT 
GCCTTCATAC AAGCTGAGCT TTCAGTGCAA AGGCCAGAGA CTGAGATAAA TGCTGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32b ( XM_005161263.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161320.1 Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X6, mRNA.

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CloneID	ODa25889
Clone ID Related Accession (Same CDS sequence)	XM_005161263.3 , XM_005161263.4 , XM_021469081.1
Accession Version	XM_005161263.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho GTPase-activating protein 32 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161263.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCCA GGCCGACCAA GCAGAAGCTG AAGCAAAGAG GTATCCTCAG GGAGAGGGTG 
TTCGGCTGTG ACCTAGGAGA GCATCTCCTC AACTCTGGCC ATGATGTCCC CCAGGTACTC 
AAGAGCTGCA CCGAGTTCAT TGAGAAGCAT GGAGTGGTTG ATGGAATGTA TCGTCTCTCT 
GGGATCGCCT CCAACATTCA GAAGCTGCGG CACGAGTTTG ATTCTGAACA GATCCCAGAT 
CTGACAAAAG ATGTTTACAT TCAGGATATA CACTGCGTGG GCTCCCTGTG CAAGCTCTAC 
TTTCGAGAGC TTCCCAACCC ACTTCTCACC TACCAGCTGT ACGAGAAATT CTCGGAGGCT 
GTGTCAGCAG CCACAGACGA GGAGAGGCTG ATCAAAATAC ATGACGTCAT TCAGCAGCTG 
CCGCCTCCGC ATTATAGAAC CCTTGAGTTT CTTATGAGGC ATCTTTCACA CTTGGCAACC 
TTCAGCTATG TAACGAACAT GCATACTAAA AATTTGGCCA TCGTTTGGGC CCCCAACTTA 
CTGAGGTCAA AGCAAATAGA ATCTGCTTGC TTCAGTGGGA CAGCAGCCTT CATGGAAGTA 
CGAATACAAT CGGTTGTGGT GGAGTTCATC CTGAATCATG TGGATGTTCT CTTCAGTCCC 
AAACTGAGCT CTCTTATTCG AGAGGGAACA GGACACAACT CTTTGTCTCG GCCTAAGTCT 
TTGCTAGTAC CGTCTCCATC CACAAAGCTG CTAACTCTAG AAGAGGCACA GGCACGCACA 
CAGGCCCAGA TCAACTCACC CATCACTGCA GACAGCAAGT ACATAGAGGT CGGAGAGGGT 
CCTGCAGCAT TACAAGGAAA ATTCCACACA GTCATAGAAT TCCCTACTGA GAGGAAGAGG 
CAGCCCATGA AGTCTAAGAA GTCTCCAGTT GGCAGTTGGC GTTCTTTTTT CAACCTGGGC 
AAGTCTTCTT CAATGTCGAA ACGCAAACTA CAGCGTAATC CCAGTGAACC AAATGAACTC 
AAGGCTATGG CACTGGCTGG AGGAAGAGGA GACACTGGTA CTCTTCGGTC AGCCAAAAGT 
GAAGAGTCTC TCACCTCTCT ACATAATGTT GAAGGAGAGT CTAAAGTGTA TAGGCCCCGC 
AGACCTCGCT CCAGCAGTGA TGCCCTCTCT GCTTCATTCA ATGGTGACCT TCTGGATACC 
AGACAGCACT GTAACTCGTA CGACAACCTA GGTCATGGAG ACAGTGATGG GGATGATGGT 
CCAATCTGTG TGCCTGCTCT CATCTCCCCA CCACGCTCCG CTGATGATGT GGATCTGAGC 
CCCCCTGACA TTGGCATGGC CTCTTTAGAC TTTGACCCCA TGTCTTTCCA ATGTAGCCTT 
CCTCTGGCAG AAACTTCGAT TTTCTCAATT GACGCAGATA CAAGCAGTCT AAAAAGGAGC 
CCTGCCAGTG GCTCAGAGTC AGTCTCGCCA GTCCGAGCTA AAGTTACGAG CCGGTTTTTG 
TCTCCAGACC ACAGTACAGC CTTAAAGGAT AAACATAAAC TGCTCCTTAC ATCTTTCTCT 
GAGAAAACTC CAGTTCAGTC TGTTGAAAGC ACCGAGATGT CAGCAGTTGT TTCTCCTCCA 
AGCATCTCTG AATCAGCTGC CTCCGTGATA ATGAAAGGAC CAGAGAGCAC TGTGCTTCAG 
CCCTCTTCCC CTCCATCTCC ACTCGACTCT CCTACAAAAT TGAGCTCTCT GTCAAGAACC 
ACCGATCTGC CCCTGAGTGA AGCCATTCAA CAAGAGCTTC TTTCTAAAGC TGCTACCTTT 
GAGAGTAAAG ACATATCGAG CATTGAATGT TCACAACCAC CACAGGTCAC CTGCTCTCAA 
GAACAGCCAG GAGCCGTAGC TTTGGACTCT CCAAAGGGCA ATGTCCCTGT CAGCTCTGTG 
TCCCTCGTCC CTCCCCCTCC CCCACCAAAG AACGCTGCCC GCATGCTTGC ACTAGCTCTA 
GCTGAATCTG CCCAGCAGGC CACCATTCTT TCTCAGAAGC AATCCTCTGG ACCCCCTACA 
CCAGTATCCC CTCTTAGGCC TCAAGAGTTA TTGGAGACCC TGGACTGGCC GCTGCCTTCT 
TCCTTGCCAT CACAGCCATC CGAGGAGGAA ACTGCAGTAG AATCATCAAA TAAATCGGTT 
CCTTTGTCTT CACCACCCTC CACTCCACCT GAAAAAATGA CTCGCTCAGT CAGTCTGCAC 
CTCACCACAG CTGATAGTGG GAAGCCATCC AGCATTTCAT GCAACAGCCA GGATGTAATC 
TCACCAGAGA CCCCAAGCCA GAGTGCTATG CCCTCTTGTC TGACTTCTCA TGTTTCTCAA 
ACACAAAAAA GTCCAGAAAA ACAGCAGCCT GCTGTCGGTC AGACACCTGT GAGCACAGCA 
AAAACCACCA CCACAACCAC CACTTCAACC ATCAGCAGTA AGGATGCTGT CATTCAACAA 
CCCACCTCTG AGATCCATAT GCCTGAAACA GTCCTGCCAC CAAAACCAAA CAAGGTGCCA 
GATCAACCAG TGGCAATTCT TCAGCCTCAG CCTCAAATGC AGACGCAACC TCATACTGCA 
TTTCGGTCTC AACCTCAAGT TCAGCCTCTT CCTCAGTCAC AAGCACAGCC ACAGTCTCAG 
CCCAGATCAC AGCCCAATGC TAGAGTTGCA CCAACCCTGG CAGCACCTCA TGATCCTGTG 
GAAAAACCAT GGGAAGCCAT AAAGCCTGTC CATCCAAAAG GAGATAGTGC TTCTCAACAA 
CACCACACTC AGGGAGTGAC ACCACCAACA CCCCCTATTC GCTCGATTGA AAGTAAACTA 
GCAGCAGCTG CACTTTGCAA CACCGAGGCT GCAAACCCTG CCAACTTTCA TGCCATGTTA 
GAGACATCCA TGAGGGGATC AGTAGAGGAG ACTCCCCCAC AGTCTCATCC GCCTCACCGC 
AGATCGTCTA CTCATCAGTC AGGTTATCTT TATCACACAA AAGGGGAGGC AGCCTTACTG 
GACCCCACCA CAGAGGCATA TTACCATCAG CGGGTAACCC CTTCAGGTCA GGCTACAGTG 
GGGTACCATT ACCGGCCAGA GAGTGTTCCA CCTCACATTT CTTACGTGTC AAAATCAGAG 
CCACACATTT CCTACAGAGG TCATGGGGAT GGTCGATACA ATACAATTGG ACCTAGATCC 
TACCACCAGT CTATCAAATC TCGTGGTTCA CTTAGAAGTG AGTACATCTC TCCAAGCTCA 
TTACACCACT CCCATAGCTA CAGCCAAGCA GATGGACCAA CCGTCTACCC AACAATTCGC 
AGGGTCCACT CCTTACATGT GCCTCCCACT GCTATTCGCT CAATGCCAGT CACCAGAACT 
GAGGTACCAC CAGATGATGA GTTATTTTAC TACCAGCGCC CAATCTACCA CTGCAAGTCT 
CACCAACCAT CATCTCAAGT CGACTATCAC GTCACACAAC TGCAGCCTTA TTTTGAAAAT 
GGAAGAGTTC AGTACCGCTA CAGCCCCTAC TCTGGCTCTC ACGTTCTGGA ATCCCCTTTC 
TATGATGTGG ACCATTATGG TACTATCCGC TTAAGGCACG TCCATTCCTT CAGTGGTCGG 
GACAGTGCCC CTCTCCTTCG ATCTGGAGCA AAATCTGGAG GCTACCACTA CCTTTCACGG 
CATGTTATTC CTTTAAAGGA GCATGGCTTC ATAAGCAGGG ACATGCCACC TTACAGTGGT 
TCAAAAGGGA GTATTTACTT TGCATGGGAC CCAGAGGAGG CAGAAAGGCT GCGCATACAC 
TCCATCCGTA GGGAAAGCAG AGCAAGGCAA AAGGTTAAGG GTTCTGTTAT GTCTCAATAC 
GACAATGTAG GACCCTACAT GCCTGTGGAT GTTGACATGT TACATTTGCG CAGCAAATCA 
GATCCTGGAA AAACTGTATT GGTGTCCTCG GAGAGCAAAG AGGCCAGATA CAATGTTGTG 
CCTGGATCTC AGCATGCTCC CAGACACTTG ATCTCTGATC CAGATGTCCT AATGTATATG 
GAAACAGAGA AGCACTGCCA AGGAAATGGA ATGGGGGAGA AAACCACCAA GCAAGGCAGC 
TCTAGGACCT ACTCGTCTAT ACATTCACAT CAATCCCAGC TTCCACCACT AAGCCGGCAG 
CACTTACCAG AGGGTGGCCA TCATGACCCA AAGTTTGAAC CAGGGGAAAA GCAACTGAGT 
GGTAAACATT GGCAGGAACA TTCGGACAGC AGGAGTGCTC AAGCACGTTA TGAAAGGTCT 
GATTTGGATC GCCACATATG CAGGGTGAAG GCCACTAGCA GTGCCAGTGA AGATGAACAA 
GCTGCTCCTG TGAAGCCGGC TCCTCCACCT AAGCCCGAGA GATCCCATAG TGTTCGAGAG 
CGGCACCATT ACAACCAGTC CAGTCTTCCT GCATATTTAC CAGACAATTC AGATAGAGAC 
CACAGTGGAA CATACTCCCA TCAGTCACAG GGGCAGAGAC AAAGCACCGT TACTTTGCAG 
TCGCATTACG ACAACCTGGA TGACTATCAT CCAGTACCTC AATCCCAAAC CACGCTAGCA 
AATCGGGGTT GTTCCAGTTC CTATCATAGC CCCGGTTTCT CAACTCCACA CAGTAATCGT 
GCATACTCCA CTGCCTTGGG ACAGGGTGCC TTCATACAAG CTGAGCTTTC AGTGCAAAGG 
CCAGAGACTG AGATAAATGC TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161320.1
CDS	666..5372
Translation	MKSRPTKQKLKQRGILRERVFGCDLGEHLLNSGHDVPQVLKSCTEFIEKHGVVDGMYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSEAVSAATDEERLIKIHDVIQQLPPPHYRTLEFLMRHLSHLATFSYVTNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVDVLFSPKLSSLIREGTGHNSLSRPKSLLVPSPSTKLLTLEEAQARTQAQINSPITADSKYIEVGEGPAALQGKFHTVIEFPTERKRQPMKSKKSPVGSWRSFFNLGKSSSMSKRKLQRNPSEPNELKAMALAGGRGDTGTLRSAKSEESLTSLHNVEGESKVYRPRRPRSSSDALSASFNGDLLDTRQHCNSYDNLGHGDSDGDDGPICVPALISPPRSADDVDLSPPDIGMASLDFDPMSFQCSLPLAETSIFSIDADTSSLKRSPASGSESVSPVRAKVTSRFLSPDHSTALKDKHKLLLTSFSEKTPVQSVESTEMSAVVSPPSISESAASVIMKGPESTVLQPSSPPSPLDSPTKLSSLSRTTDLPLSEAIQQELLSKAATFESKDISSIECSQPPQVTCSQEQPGAVALDSPKGNVPVSSVSLVPPPPPPKNAARMLALALAESAQQATILSQKQSSGPPTPVSPLRPQELLETLDWPLPSSLPSQPSEEETAVESSNKSVPLSSPPSTPPEKMTRSVSLHLTTADSGKPSSISCNSQDVISPETPSQSAMPSCLTSHVSQTQKSPEKQQPAVGQTPVSTAKTTTTTTTSTISSKDAVIQQPTSEIHMPETVLPPKPNKVPDQPVAILQPQPQMQTQPHTAFRSQPQVQPLPQSQAQPQSQPRSQPNARVAPTLAAPHDPVEKPWEAIKPVHPKGDSASQQHHTQGVTPPTPPIRSIESKLAAAALCNTEAANPANFHAMLETSMRGSVEETPPQSHPPHRRSSTHQSGYLYHTKGEAALLDPTTEAYYHQRVTPSGQATVGYHYRPESVPPHISYVSKSEPHISYRGHGDGRYNTIGPRSYHQSIKSRGSLRSEYISPSSLHHSHSYSQADGPTVYPTIRRVHSLHVPPTAIRSMPVTRTEVPPDDELFYYQRPIYHCKSHQPSSQVDYHVTQLQPYFENGRVQYRYSPYSGSHVLESPFYDVDHYGTIRLRHVHSFSGRDSAPLLRSGAKSGGYHYLSRHVIPLKEHGFISRDMPPYSGSKGSIYFAWDPEEAERLRIHSIRRESRARQKVKGSVMSQYDNVGPYMPVDVDMLHLRSKSDPGKTVLVSSESKEARYNVVPGSQHAPRHLISDPDVLMYMETEKHCQGNGMGEKTTKQGSSRTYSSIHSHQSQLPPLSRQHLPEGGHHDPKFEPGEKQLSGKHWQEHSDSRSAQARYERSDLDRHICRVKATSSASEDEQAAPVKPAPPPKPERSHSVRERHHYNQSSLPAYLPDNSDRDHSGTYSHQSQGQRQSTVTLQSHYDNLDDYHPVPQSQTTLANRGCSSSYHSPGFSTPHSNRAYSTALGQGAFIQAELSVQRPETEINAE

Target ORF information:

RefSeq Version	XM_005161263.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161263.3

ORF Insert Sequence:

ATGAAGTCCA GGCCGACCAA GCAGAAGCTG AAGCAAAGAG GTATCCTCAG GGAGAGGGTG 
TTCGGCTGTG ACCTAGGAGA GCATCTCCTC AACTCTGGCC ATGATGTCCC CCAGGTACTC 
AAGAGCTGCA CCGAGTTCAT TGAGAAGCAT GGAGTGGTTG ATGGAATGTA TCGTCTCTCT 
GGGATCGCCT CCAACATTCA GAAGCTGCGG CACGAGTTTG ATTCTGAACA GATCCCAGAT 
CTGACAAAAG ATGTTTACAT TCAGGATATA CACTGCGTGG GCTCCCTGTG CAAGCTCTAC 
TTTCGAGAGC TTCCCAACCC ACTTCTCACC TACCAGCTGT ACGAGAAATT CTCGGAGGCT 
GTGTCAGCAG CCACAGACGA GGAGAGGCTG ATCAAAATAC ATGACGTCAT TCAGCAGCTG 
CCGCCTCCGC ATTATAGAAC CCTTGAGTTT CTTATGAGGC ATCTTTCACA CTTGGCAACC 
TTCAGCTATG TAACGAACAT GCATACTAAA AATTTGGCCA TCGTTTGGGC CCCCAACTTA 
CTGAGGTCAA AGCAAATAGA ATCTGCTTGC TTCAGTGGGA CAGCAGCCTT CATGGAAGTA 
CGAATACAAT CGGTTGTGGT GGAGTTCATC CTGAATCATG TGGATGTTCT CTTCAGTCCC 
AAACTGAGCT CTCTTATTCG AGAGGGAACA GGACACAACT CTTTGTCTCG GCCTAAGTCT 
TTGCTAGTAC CGTCTCCATC CACAAAGCTG CTAACTCTAG AAGAGGCACA GGCACGCACA 
CAGGCCCAGA TCAACTCACC CATCACTGCA GACAGCAAGT ACATAGAGGT CGGAGAGGGT 
CCTGCAGCAT TACAAGGAAA ATTCCACACA GTCATAGAAT TCCCTACTGA GAGGAAGAGG 
CAGCCCATGA AGTCTAAGAA GTCTCCAGTT GGCAGTTGGC GTTCTTTTTT CAACCTGGGC 
AAGTCTTCTT CAATGTCGAA ACGCAAACTA CAGCGTAATC CCAGTGAACC AAATGAACTC 
AAGGCTATGG CACTGGCTGG AGGAAGAGGA GACACTGGTA CTCTTCGGTC AGCCAAAAGT 
GAAGAGTCTC TCACCTCTCT ACATAATGTT GAAGGAGAGT CTAAAGTGTA TAGGCCCCGC 
AGACCTCGCT CCAGCAGTGA TGCCCTCTCT GCTTCATTCA ATGGTGACCT TCTGGATACC 
AGACAGCACT GTAACTCGTA CGACAACCTA GGTCATGGAG ACAGTGATGG GGATGATGGT 
CCAATCTGTG TGCCTGCTCT CATCTCCCCA CCACGCTCCG CTGATGATGT GGATCTGAGC 
CCCCCTGACA TTGGCATGGC CTCTTTAGAC TTTGACCCCA TGTCTTTCCA ATGTAGCCTT 
CCTCTGGCAG AAACTTCGAT TTTCTCAATT GACGCAGATA CAAGCAGTCT AAAAAGGAGC 
CCTGCCAGTG GCTCAGAGTC AGTCTCGCCA GTCCGAGCTA AAGTTACGAG CCGGTTTTTG 
TCTCCAGACC ACAGTACAGC CTTAAAGGAT AAACATAAAC TGCTCCTTAC ATCTTTCTCT 
GAGAAAACTC CAGTTCAGTC TGTTGAAAGC ACCGAGATGT CAGCAGTTGT TTCTCCTCCA 
AGCATCTCTG AATCAGCTGC CTCCGTGATA ATGAAAGGAC CAGAGAGCAC TGTGCTTCAG 
CCCTCTTCCC CTCCATCTCC ACTCGACTCT CCTACAAAAT TGAGCTCTCT GTCAAGAACC 
ACCGATCTGC CCCTGAGTGA AGCCATTCAA CAAGAGCTTC TTTCTAAAGC TGCTACCTTT 
GAGAGTAAAG ACATATCGAG CATTGAATGT TCACAACCAC CACAGGTCAC CTGCTCTCAA 
GAACAGCCAG GAGCCGTAGC TTTGGACTCT CCAAAGGGCA ATGTCCCTGT CAGCTCTGTG 
TCCCTCGTCC CTCCCCCTCC CCCACCAAAG AACGCTGCCC GCATGCTTGC ACTAGCTCTA 
GCTGAATCTG CCCAGCAGGC CACCATTCTT TCTCAGAAGC AATCCTCTGG ACCCCCTACA 
CCAGTATCCC CTCTTAGGCC TCAAGAGTTA TTGGAGACCC TGGACTGGCC GCTGCCTTCT 
TCCTTGCCAT CACAGCCATC CGAGGAGGAA ACTGCAGTAG AATCATCAAA TAAATCGGTT 
CCTTTGTCTT CACCACCCTC CACTCCACCT GAAAAAATGA CTCGCTCAGT CAGTCTGCAC 
CTCACCACAG CTGATAGTGG GAAGCCATCC AGCATTTCAT GCAACAGCCA GGATGTAATC 
TCACCAGAGA CCCCAAGCCA GAGTGCTATG CCCTCTTGTC TGACTTCTCA TGTTTCTCAA 
ACACAAAAAA GTCCAGAAAA ACAGCAGCCT GCTGTCGGTC AGACACCTGT GAGCACAGCA 
AAAACCACCA CCACAACCAC CACTTCAACC ATCAGCAGTA AGGATGCTGT CATTCAACAA 
CCCACCTCTG AGATCCATAT GCCTGAAACA GTCCTGCCAC CAAAACCAAA CAAGGTGCCA 
GATCAACCAG TGGCAATTCT TCAGCCTCAG CCTCAAATGC AGACGCAACC TCATACTGCA 
TTTCGGTCTC AACCTCAAGT TCAGCCTCTT CCTCAGTCAC AAGCACAGCC ACAGTCTCAG 
CCCAGATCAC AGCCCAATGC TAGAGTTGCA CCAACCCTGG CAGCACCTCA TGATCCTGTG 
GAAAAACCAT GGGAAGCCAT AAAGCCTGTC CATCCAAAAG GAGATAGTGC TTCTCAACAA 
CACCACACTC AGGGAGTGAC ACCACCAACA CCCCCTATTC GCTCGATTGA AAGTAAACTA 
GCAGCAGCTG CACTTTGCAA CACCGAGGCT GCAAACCCTG CCAACTTTCA TGCCATGTTA 
GAGACATCCA TGAGGGGATC AGTAGAGGAG ACTCCCCCAC AGTCTCATCC GCCTCACCGC 
AGATCGTCTA CTCATCAGTC AGGTTATCTT TATCACACAA AAGGGGAGGC AGCCTTACTG 
GACCCCACCA CAGAGGCATA TTACCATCAG CGGGTAACCC CTTCAGGTCA GGCTACAGTG 
GGGTACCATT ACCGGCCAGA GAGTGTTCCA CCTCACATTT CTTACGTGTC AAAATCAGAG 
CCACACATTT CCTACAGAGG TCATGGGGAT GGTCGATACA ATACAATTGG ACCTAGATCC 
TACCACCAGT CTATCAAATC TCGTGGTTCA CTTAGAAGTG AGTACATCTC TCCAAGCTCA 
TTACACCACT CCCATAGCTA CAGCCAAGCA GATGGACCAA CCGTCTACCC AACAATTCGC 
AGGGTCCACT CCTTACATGT GCCTCCCACT GCTATTCGCT CAATGCCAGT CACCAGAACT 
GAGGTACCAC CAGATGATGA GTTATTTTAC TACCAGCGCC CAATCTACCA CTGCAAGTCT 
CACCAACCAT CATCTCAAGT CGACTATCAC GTCACACAAC TGCAGCCTTA TTTTGAAAAT 
GGAAGAGTTC AGTACCGCTA CAGCCCCTAC TCTGGCTCTC ACGTTCTGGA ATCCCCTTTC 
TATGATGTGG ACCATTATGG TACTATCCGC TTAAGGCACG TCCATTCCTT CAGTGGTCGG 
GACAGTGCCC CTCTCCTTCG ATCTGGAGCA AAATCTGGAG GCTACCACTA CCTTTCACGG 
CATGTTATTC CTTTAAAGGA GCATGGCTTC ATAAGCAGGG ACATGCCACC TTACAGTGGT 
TCAAAAGGGA GTATTTACTT TGCATGGGAC CCAGAGGAGG CAGAAAGGCT GCGCATACAC 
TCCATCCGTA GGGAAAGCAG AGCAAGGCAA AAGGTTAAGG GTTCTGTTAT GTCTCAATAC 
GACAATGTAG GACCCTACAT GCCTGTGGAT GTTGACATGT TACATTTGCG CAGCAAATCA 
GATCCTGGAA AAACTGTATT GGTGTCCTCG GAGAGCAAAG AGGCCAGATA CAATGTTGTG 
CCTGGATCTC AGCATGCTCC CAGACACTTG ATCTCTGATC CAGATGTCCT AATGTATATG 
GAAACAGAGA AGCACTGCCA AGGAAATGGA ATGGGGGAGA AAACCACCAA GCAAGGCAGC 
TCTAGGACCT ACTCGTCTAT ACATTCACAT CAATCCCAGC TTCCACCACT AAGCCGGCAG 
CACTTACCAG AGGGTGGCCA TCATGACCCA AAGTTTGAAC CAGGGGAAAA GCAACTGAGT 
GGTAAACATT GGCAGGAACA TTCGGACAGC AGGAGTGCTC AAGCACGTTA TGAAAGGTCT 
GATTTGGATC GCCACATATG CAGGGTGAAG GCCACTAGCA GTGCCAGTGA AGATGAACAA 
GCTGCTCCTG TGAAGCCGGC TCCTCCACCT AAGCCCGAGA GATCCCATAG TGTTCGAGAG 
CGGCACCATT ACAACCAGTC CAGTCTTCCT GCATATTTAC CAGACAATTC AGATAGAGAC 
CACAGTGGAA CATACTCCCA TCAGTCACAG GGGCAGAGAC AAAGCACCGT TACTTTGCAG 
TCGCATTACG ACAACCTGGA TGACTATCAT CCAGTACCTC AATCCCAAAC CACGCTAGCA 
AATCGGGGTT GTTCCAGTTC CTATCATAGC CCCGGTTTCT CAACTCCACA CAGTAATCGT 
GCATACTCCA CTGCCTTGGG ACAGGGTGCC TTCATACAAG CTGAGCTTTC AGTGCAAAGG 
CCAGAGACTG AGATAAATGC TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32b ( XM_005161263.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005161320.1 Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ODa25889
Clone ID Related Accession (Same CDS sequence)	XM_005161263.3 , XM_005161263.4 , XM_021469081.1
Accession Version	XM_005161263.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho GTPase-activating protein 32 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161263.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCCA GGCCGACCAA GCAGAAGCTG AAGCAAAGAG GTATCCTCAG GGAGAGGGTG 
TTCGGCTGTG ACCTAGGAGA GCATCTCCTC AACTCTGGCC ATGATGTCCC CCAGGTACTC 
AAGAGCTGCA CCGAGTTCAT TGAGAAGCAT GGAGTGGTTG ATGGAATGTA TCGTCTCTCT 
GGGATCGCCT CCAACATTCA GAAGCTGCGG CACGAGTTTG ATTCTGAACA GATCCCAGAT 
CTGACAAAAG ATGTTTACAT TCAGGATATA CACTGCGTGG GCTCCCTGTG CAAGCTCTAC 
TTTCGAGAGC TTCCCAACCC ACTTCTCACC TACCAGCTGT ACGAGAAATT CTCGGAGGCT 
GTGTCAGCAG CCACAGACGA GGAGAGGCTG ATCAAAATAC ATGACGTCAT TCAGCAGCTG 
CCGCCTCCGC ATTATAGAAC CCTTGAGTTT CTTATGAGGC ATCTTTCACA CTTGGCAACC 
TTCAGCTATG TAACGAACAT GCATACTAAA AATTTGGCCA TCGTTTGGGC CCCCAACTTA 
CTGAGGTCAA AGCAAATAGA ATCTGCTTGC TTCAGTGGGA CAGCAGCCTT CATGGAAGTA 
CGAATACAAT CGGTTGTGGT GGAGTTCATC CTGAATCATG TGGATGTTCT CTTCAGTCCC 
AAACTGAGCT CTCTTATTCG AGAGGGAACA GGACACAACT CTTTGTCTCG GCCTAAGTCT 
TTGCTAGTAC CGTCTCCATC CACAAAGCTG CTAACTCTAG AAGAGGCACA GGCACGCACA 
CAGGCCCAGA TCAACTCACC CATCACTGCA GACAGCAAGT ACATAGAGGT CGGAGAGGGT 
CCTGCAGCAT TACAAGGAAA ATTCCACACA GTCATAGAAT TCCCTACTGA GAGGAAGAGG 
CAGCCCATGA AGTCTAAGAA GTCTCCAGTT GGCAGTTGGC GTTCTTTTTT CAACCTGGGC 
AAGTCTTCTT CAATGTCGAA ACGCAAACTA CAGCGTAATC CCAGTGAACC AAATGAACTC 
AAGGCTATGG CACTGGCTGG AGGAAGAGGA GACACTGGTA CTCTTCGGTC AGCCAAAAGT 
GAAGAGTCTC TCACCTCTCT ACATAATGTT GAAGGAGAGT CTAAAGTGTA TAGGCCCCGC 
AGACCTCGCT CCAGCAGTGA TGCCCTCTCT GCTTCATTCA ATGGTGACCT TCTGGATACC 
AGACAGCACT GTAACTCGTA CGACAACCTA GGTCATGGAG ACAGTGATGG GGATGATGGT 
CCAATCTGTG TGCCTGCTCT CATCTCCCCA CCACGCTCCG CTGATGATGT GGATCTGAGC 
CCCCCTGACA TTGGCATGGC CTCTTTAGAC TTTGACCCCA TGTCTTTCCA ATGTAGCCTT 
CCTCTGGCAG AAACTTCGAT TTTCTCAATT GACGCAGATA CAAGCAGTCT AAAAAGGAGC 
CCTGCCAGTG GCTCAGAGTC AGTCTCGCCA GTCCGAGCTA AAGTTACGAG CCGGTTTTTG 
TCTCCAGACC ACAGTACAGC CTTAAAGGAT AAACATAAAC TGCTCCTTAC ATCTTTCTCT 
GAGAAAACTC CAGTTCAGTC TGTTGAAAGC ACCGAGATGT CAGCAGTTGT TTCTCCTCCA 
AGCATCTCTG AATCAGCTGC CTCCGTGATA ATGAAAGGAC CAGAGAGCAC TGTGCTTCAG 
CCCTCTTCCC CTCCATCTCC ACTCGACTCT CCTACAAAAT TGAGCTCTCT GTCAAGAACC 
ACCGATCTGC CCCTGAGTGA AGCCATTCAA CAAGAGCTTC TTTCTAAAGC TGCTACCTTT 
GAGAGTAAAG ACATATCGAG CATTGAATGT TCACAACCAC CACAGGTCAC CTGCTCTCAA 
GAACAGCCAG GAGCCGTAGC TTTGGACTCT CCAAAGGGCA ATGTCCCTGT CAGCTCTGTG 
TCCCTCGTCC CTCCCCCTCC CCCACCAAAG AACGCTGCCC GCATGCTTGC ACTAGCTCTA 
GCTGAATCTG CCCAGCAGGC CACCATTCTT TCTCAGAAGC AATCCTCTGG ACCCCCTACA 
CCAGTATCCC CTCTTAGGCC TCAAGAGTTA TTGGAGACCC TGGACTGGCC GCTGCCTTCT 
TCCTTGCCAT CACAGCCATC CGAGGAGGAA ACTGCAGTAG AATCATCAAA TAAATCGGTT 
CCTTTGTCTT CACCACCCTC CACTCCACCT GAAAAAATGA CTCGCTCAGT CAGTCTGCAC 
CTCACCACAG CTGATAGTGG GAAGCCATCC AGCATTTCAT GCAACAGCCA GGATGTAATC 
TCACCAGAGA CCCCAAGCCA GAGTGCTATG CCCTCTTGTC TGACTTCTCA TGTTTCTCAA 
ACACAAAAAA GTCCAGAAAA ACAGCAGCCT GCTGTCGGTC AGACACCTGT GAGCACAGCA 
AAAACCACCA CCACAACCAC CACTTCAACC ATCAGCAGTA AGGATGCTGT CATTCAACAA 
CCCACCTCTG AGATCCATAT GCCTGAAACA GTCCTGCCAC CAAAACCAAA CAAGGTGCCA 
GATCAACCAG TGGCAATTCT TCAGCCTCAG CCTCAAATGC AGACGCAACC TCATACTGCA 
TTTCGGTCTC AACCTCAAGT TCAGCCTCTT CCTCAGTCAC AAGCACAGCC ACAGTCTCAG 
CCCAGATCAC AGCCCAATGC TAGAGTTGCA CCAACCCTGG CAGCACCTCA TGATCCTGTG 
GAAAAACCAT GGGAAGCCAT AAAGCCTGTC CATCCAAAAG GAGATAGTGC TTCTCAACAA 
CACCACACTC AGGGAGTGAC ACCACCAACA CCCCCTATTC GCTCGATTGA AAGTAAACTA 
GCAGCAGCTG CACTTTGCAA CACCGAGGCT GCAAACCCTG CCAACTTTCA TGCCATGTTA 
GAGACATCCA TGAGGGGATC AGTAGAGGAG ACTCCCCCAC AGTCTCATCC GCCTCACCGC 
AGATCGTCTA CTCATCAGTC AGGTTATCTT TATCACACAA AAGGGGAGGC AGCCTTACTG 
GACCCCACCA CAGAGGCATA TTACCATCAG CGGGTAACCC CTTCAGGTCA GGCTACAGTG 
GGGTACCATT ACCGGCCAGA GAGTGTTCCA CCTCACATTT CTTACGTGTC AAAATCAGAG 
CCACACATTT CCTACAGAGG TCATGGGGAT GGTCGATACA ATACAATTGG ACCTAGATCC 
TACCACCAGT CTATCAAATC TCGTGGTTCA CTTAGAAGTG AGTACATCTC TCCAAGCTCA 
TTACACCACT CCCATAGCTA CAGCCAAGCA GATGGACCAA CCGTCTACCC AACAATTCGC 
AGGGTCCACT CCTTACATGT GCCTCCCACT GCTATTCGCT CAATGCCAGT CACCAGAACT 
GAGGTACCAC CAGATGATGA GTTATTTTAC TACCAGCGCC CAATCTACCA CTGCAAGTCT 
CACCAACCAT CATCTCAAGT CGACTATCAC GTCACACAAC TGCAGCCTTA TTTTGAAAAT 
GGAAGAGTTC AGTACCGCTA CAGCCCCTAC TCTGGCTCTC ACGTTCTGGA ATCCCCTTTC 
TATGATGTGG ACCATTATGG TACTATCCGC TTAAGGCACG TCCATTCCTT CAGTGGTCGG 
GACAGTGCCC CTCTCCTTCG ATCTGGAGCA AAATCTGGAG GCTACCACTA CCTTTCACGG 
CATGTTATTC CTTTAAAGGA GCATGGCTTC ATAAGCAGGG ACATGCCACC TTACAGTGGT 
TCAAAAGGGA GTATTTACTT TGCATGGGAC CCAGAGGAGG CAGAAAGGCT GCGCATACAC 
TCCATCCGTA GGGAAAGCAG AGCAAGGCAA AAGGTTAAGG GTTCTGTTAT GTCTCAATAC 
GACAATGTAG GACCCTACAT GCCTGTGGAT GTTGACATGT TACATTTGCG CAGCAAATCA 
GATCCTGGAA AAACTGTATT GGTGTCCTCG GAGAGCAAAG AGGCCAGATA CAATGTTGTG 
CCTGGATCTC AGCATGCTCC CAGACACTTG ATCTCTGATC CAGATGTCCT AATGTATATG 
GAAACAGAGA AGCACTGCCA AGGAAATGGA ATGGGGGAGA AAACCACCAA GCAAGGCAGC 
TCTAGGACCT ACTCGTCTAT ACATTCACAT CAATCCCAGC TTCCACCACT AAGCCGGCAG 
CACTTACCAG AGGGTGGCCA TCATGACCCA AAGTTTGAAC CAGGGGAAAA GCAACTGAGT 
GGTAAACATT GGCAGGAACA TTCGGACAGC AGGAGTGCTC AAGCACGTTA TGAAAGGTCT 
GATTTGGATC GCCACATATG CAGGGTGAAG GCCACTAGCA GTGCCAGTGA AGATGAACAA 
GCTGCTCCTG TGAAGCCGGC TCCTCCACCT AAGCCCGAGA GATCCCATAG TGTTCGAGAG 
CGGCACCATT ACAACCAGTC CAGTCTTCCT GCATATTTAC CAGACAATTC AGATAGAGAC 
CACAGTGGAA CATACTCCCA TCAGTCACAG GGGCAGAGAC AAAGCACCGT TACTTTGCAG 
TCGCATTACG ACAACCTGGA TGACTATCAT CCAGTACCTC AATCCCAAAC CACGCTAGCA 
AATCGGGGTT GTTCCAGTTC CTATCATAGC CCCGGTTTCT CAACTCCACA CAGTAATCGT 
GCATACTCCA CTGCCTTGGG ACAGGGTGCC TTCATACAAG CTGAGCTTTC AGTGCAAAGG 
CCAGAGACTG AGATAAATGC TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005161320.1
CDS	534..5240
Translation	MKSRPTKQKLKQRGILRERVFGCDLGEHLLNSGHDVPQVLKSCTEFIEKHGVVDGMYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSEAVSAATDEERLIKIHDVIQQLPPPHYRTLEFLMRHLSHLATFSYVTNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVDVLFSPKLSSLIREGTGHNSLSRPKSLLVPSPSTKLLTLEEAQARTQAQINSPITADSKYIEVGEGPAALQGKFHTVIEFPTERKRQPMKSKKSPVGSWRSFFNLGKSSSMSKRKLQRNPSEPNELKAMALAGGRGDTGTLRSAKSEESLTSLHNVEGESKVYRPRRPRSSSDALSASFNGDLLDTRQHCNSYDNLGHGDSDGDDGPICVPALISPPRSADDVDLSPPDIGMASLDFDPMSFQCSLPLAETSIFSIDADTSSLKRSPASGSESVSPVRAKVTSRFLSPDHSTALKDKHKLLLTSFSEKTPVQSVESTEMSAVVSPPSISESAASVIMKGPESTVLQPSSPPSPLDSPTKLSSLSRTTDLPLSEAIQQELLSKAATFESKDISSIECSQPPQVTCSQEQPGAVALDSPKGNVPVSSVSLVPPPPPPKNAARMLALALAESAQQATILSQKQSSGPPTPVSPLRPQELLETLDWPLPSSLPSQPSEEETAVESSNKSVPLSSPPSTPPEKMTRSVSLHLTTADSGKPSSISCNSQDVISPETPSQSAMPSCLTSHVSQTQKSPEKQQPAVGQTPVSTAKTTTTTTTSTISSKDAVIQQPTSEIHMPETVLPPKPNKVPDQPVAILQPQPQMQTQPHTAFRSQPQVQPLPQSQAQPQSQPRSQPNARVAPTLAAPHDPVEKPWEAIKPVHPKGDSASQQHHTQGVTPPTPPIRSIESKLAAAALCNTEAANPANFHAMLETSMRGSVEETPPQSHPPHRRSSTHQSGYLYHTKGEAALLDPTTEAYYHQRVTPSGQATVGYHYRPESVPPHISYVSKSEPHISYRGHGDGRYNTIGPRSYHQSIKSRGSLRSEYISPSSLHHSHSYSQADGPTVYPTIRRVHSLHVPPTAIRSMPVTRTEVPPDDELFYYQRPIYHCKSHQPSSQVDYHVTQLQPYFENGRVQYRYSPYSGSHVLESPFYDVDHYGTIRLRHVHSFSGRDSAPLLRSGAKSGGYHYLSRHVIPLKEHGFISRDMPPYSGSKGSIYFAWDPEEAERLRIHSIRRESRARQKVKGSVMSQYDNVGPYMPVDVDMLHLRSKSDPGKTVLVSSESKEARYNVVPGSQHAPRHLISDPDVLMYMETEKHCQGNGMGEKTTKQGSSRTYSSIHSHQSQLPPLSRQHLPEGGHHDPKFEPGEKQLSGKHWQEHSDSRSAQARYERSDLDRHICRVKATSSASEDEQAAPVKPAPPPKPERSHSVRERHHYNQSSLPAYLPDNSDRDHSGTYSHQSQGQRQSTVTLQSHYDNLDDYHPVPQSQTTLANRGCSSSYHSPGFSTPHSNRAYSTALGQGAFIQAELSVQRPETEINAE

Target ORF information:

RefSeq Version	XM_005161263.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161263.4

ORF Insert Sequence:

ATGAAGTCCA GGCCGACCAA GCAGAAGCTG AAGCAAAGAG GTATCCTCAG GGAGAGGGTG 
TTCGGCTGTG ACCTAGGAGA GCATCTCCTC AACTCTGGCC ATGATGTCCC CCAGGTACTC 
AAGAGCTGCA CCGAGTTCAT TGAGAAGCAT GGAGTGGTTG ATGGAATGTA TCGTCTCTCT 
GGGATCGCCT CCAACATTCA GAAGCTGCGG CACGAGTTTG ATTCTGAACA GATCCCAGAT 
CTGACAAAAG ATGTTTACAT TCAGGATATA CACTGCGTGG GCTCCCTGTG CAAGCTCTAC 
TTTCGAGAGC TTCCCAACCC ACTTCTCACC TACCAGCTGT ACGAGAAATT CTCGGAGGCT 
GTGTCAGCAG CCACAGACGA GGAGAGGCTG ATCAAAATAC ATGACGTCAT TCAGCAGCTG 
CCGCCTCCGC ATTATAGAAC CCTTGAGTTT CTTATGAGGC ATCTTTCACA CTTGGCAACC 
TTCAGCTATG TAACGAACAT GCATACTAAA AATTTGGCCA TCGTTTGGGC CCCCAACTTA 
CTGAGGTCAA AGCAAATAGA ATCTGCTTGC TTCAGTGGGA CAGCAGCCTT CATGGAAGTA 
CGAATACAAT CGGTTGTGGT GGAGTTCATC CTGAATCATG TGGATGTTCT CTTCAGTCCC 
AAACTGAGCT CTCTTATTCG AGAGGGAACA GGACACAACT CTTTGTCTCG GCCTAAGTCT 
TTGCTAGTAC CGTCTCCATC CACAAAGCTG CTAACTCTAG AAGAGGCACA GGCACGCACA 
CAGGCCCAGA TCAACTCACC CATCACTGCA GACAGCAAGT ACATAGAGGT CGGAGAGGGT 
CCTGCAGCAT TACAAGGAAA ATTCCACACA GTCATAGAAT TCCCTACTGA GAGGAAGAGG 
CAGCCCATGA AGTCTAAGAA GTCTCCAGTT GGCAGTTGGC GTTCTTTTTT CAACCTGGGC 
AAGTCTTCTT CAATGTCGAA ACGCAAACTA CAGCGTAATC CCAGTGAACC AAATGAACTC 
AAGGCTATGG CACTGGCTGG AGGAAGAGGA GACACTGGTA CTCTTCGGTC AGCCAAAAGT 
GAAGAGTCTC TCACCTCTCT ACATAATGTT GAAGGAGAGT CTAAAGTGTA TAGGCCCCGC 
AGACCTCGCT CCAGCAGTGA TGCCCTCTCT GCTTCATTCA ATGGTGACCT TCTGGATACC 
AGACAGCACT GTAACTCGTA CGACAACCTA GGTCATGGAG ACAGTGATGG GGATGATGGT 
CCAATCTGTG TGCCTGCTCT CATCTCCCCA CCACGCTCCG CTGATGATGT GGATCTGAGC 
CCCCCTGACA TTGGCATGGC CTCTTTAGAC TTTGACCCCA TGTCTTTCCA ATGTAGCCTT 
CCTCTGGCAG AAACTTCGAT TTTCTCAATT GACGCAGATA CAAGCAGTCT AAAAAGGAGC 
CCTGCCAGTG GCTCAGAGTC AGTCTCGCCA GTCCGAGCTA AAGTTACGAG CCGGTTTTTG 
TCTCCAGACC ACAGTACAGC CTTAAAGGAT AAACATAAAC TGCTCCTTAC ATCTTTCTCT 
GAGAAAACTC CAGTTCAGTC TGTTGAAAGC ACCGAGATGT CAGCAGTTGT TTCTCCTCCA 
AGCATCTCTG AATCAGCTGC CTCCGTGATA ATGAAAGGAC CAGAGAGCAC TGTGCTTCAG 
CCCTCTTCCC CTCCATCTCC ACTCGACTCT CCTACAAAAT TGAGCTCTCT GTCAAGAACC 
ACCGATCTGC CCCTGAGTGA AGCCATTCAA CAAGAGCTTC TTTCTAAAGC TGCTACCTTT 
GAGAGTAAAG ACATATCGAG CATTGAATGT TCACAACCAC CACAGGTCAC CTGCTCTCAA 
GAACAGCCAG GAGCCGTAGC TTTGGACTCT CCAAAGGGCA ATGTCCCTGT CAGCTCTGTG 
TCCCTCGTCC CTCCCCCTCC CCCACCAAAG AACGCTGCCC GCATGCTTGC ACTAGCTCTA 
GCTGAATCTG CCCAGCAGGC CACCATTCTT TCTCAGAAGC AATCCTCTGG ACCCCCTACA 
CCAGTATCCC CTCTTAGGCC TCAAGAGTTA TTGGAGACCC TGGACTGGCC GCTGCCTTCT 
TCCTTGCCAT CACAGCCATC CGAGGAGGAA ACTGCAGTAG AATCATCAAA TAAATCGGTT 
CCTTTGTCTT CACCACCCTC CACTCCACCT GAAAAAATGA CTCGCTCAGT CAGTCTGCAC 
CTCACCACAG CTGATAGTGG GAAGCCATCC AGCATTTCAT GCAACAGCCA GGATGTAATC 
TCACCAGAGA CCCCAAGCCA GAGTGCTATG CCCTCTTGTC TGACTTCTCA TGTTTCTCAA 
ACACAAAAAA GTCCAGAAAA ACAGCAGCCT GCTGTCGGTC AGACACCTGT GAGCACAGCA 
AAAACCACCA CCACAACCAC CACTTCAACC ATCAGCAGTA AGGATGCTGT CATTCAACAA 
CCCACCTCTG AGATCCATAT GCCTGAAACA GTCCTGCCAC CAAAACCAAA CAAGGTGCCA 
GATCAACCAG TGGCAATTCT TCAGCCTCAG CCTCAAATGC AGACGCAACC TCATACTGCA 
TTTCGGTCTC AACCTCAAGT TCAGCCTCTT CCTCAGTCAC AAGCACAGCC ACAGTCTCAG 
CCCAGATCAC AGCCCAATGC TAGAGTTGCA CCAACCCTGG CAGCACCTCA TGATCCTGTG 
GAAAAACCAT GGGAAGCCAT AAAGCCTGTC CATCCAAAAG GAGATAGTGC TTCTCAACAA 
CACCACACTC AGGGAGTGAC ACCACCAACA CCCCCTATTC GCTCGATTGA AAGTAAACTA 
GCAGCAGCTG CACTTTGCAA CACCGAGGCT GCAAACCCTG CCAACTTTCA TGCCATGTTA 
GAGACATCCA TGAGGGGATC AGTAGAGGAG ACTCCCCCAC AGTCTCATCC GCCTCACCGC 
AGATCGTCTA CTCATCAGTC AGGTTATCTT TATCACACAA AAGGGGAGGC AGCCTTACTG 
GACCCCACCA CAGAGGCATA TTACCATCAG CGGGTAACCC CTTCAGGTCA GGCTACAGTG 
GGGTACCATT ACCGGCCAGA GAGTGTTCCA CCTCACATTT CTTACGTGTC AAAATCAGAG 
CCACACATTT CCTACAGAGG TCATGGGGAT GGTCGATACA ATACAATTGG ACCTAGATCC 
TACCACCAGT CTATCAAATC TCGTGGTTCA CTTAGAAGTG AGTACATCTC TCCAAGCTCA 
TTACACCACT CCCATAGCTA CAGCCAAGCA GATGGACCAA CCGTCTACCC AACAATTCGC 
AGGGTCCACT CCTTACATGT GCCTCCCACT GCTATTCGCT CAATGCCAGT CACCAGAACT 
GAGGTACCAC CAGATGATGA GTTATTTTAC TACCAGCGCC CAATCTACCA CTGCAAGTCT 
CACCAACCAT CATCTCAAGT CGACTATCAC GTCACACAAC TGCAGCCTTA TTTTGAAAAT 
GGAAGAGTTC AGTACCGCTA CAGCCCCTAC TCTGGCTCTC ACGTTCTGGA ATCCCCTTTC 
TATGATGTGG ACCATTATGG TACTATCCGC TTAAGGCACG TCCATTCCTT CAGTGGTCGG 
GACAGTGCCC CTCTCCTTCG ATCTGGAGCA AAATCTGGAG GCTACCACTA CCTTTCACGG 
CATGTTATTC CTTTAAAGGA GCATGGCTTC ATAAGCAGGG ACATGCCACC TTACAGTGGT 
TCAAAAGGGA GTATTTACTT TGCATGGGAC CCAGAGGAGG CAGAAAGGCT GCGCATACAC 
TCCATCCGTA GGGAAAGCAG AGCAAGGCAA AAGGTTAAGG GTTCTGTTAT GTCTCAATAC 
GACAATGTAG GACCCTACAT GCCTGTGGAT GTTGACATGT TACATTTGCG CAGCAAATCA 
GATCCTGGAA AAACTGTATT GGTGTCCTCG GAGAGCAAAG AGGCCAGATA CAATGTTGTG 
CCTGGATCTC AGCATGCTCC CAGACACTTG ATCTCTGATC CAGATGTCCT AATGTATATG 
GAAACAGAGA AGCACTGCCA AGGAAATGGA ATGGGGGAGA AAACCACCAA GCAAGGCAGC 
TCTAGGACCT ACTCGTCTAT ACATTCACAT CAATCCCAGC TTCCACCACT AAGCCGGCAG 
CACTTACCAG AGGGTGGCCA TCATGACCCA AAGTTTGAAC CAGGGGAAAA GCAACTGAGT 
GGTAAACATT GGCAGGAACA TTCGGACAGC AGGAGTGCTC AAGCACGTTA TGAAAGGTCT 
GATTTGGATC GCCACATATG CAGGGTGAAG GCCACTAGCA GTGCCAGTGA AGATGAACAA 
GCTGCTCCTG TGAAGCCGGC TCCTCCACCT AAGCCCGAGA GATCCCATAG TGTTCGAGAG 
CGGCACCATT ACAACCAGTC CAGTCTTCCT GCATATTTAC CAGACAATTC AGATAGAGAC 
CACAGTGGAA CATACTCCCA TCAGTCACAG GGGCAGAGAC AAAGCACCGT TACTTTGCAG 
TCGCATTACG ACAACCTGGA TGACTATCAT CCAGTACCTC AATCCCAAAC CACGCTAGCA 
AATCGGGGTT GTTCCAGTTC CTATCATAGC CCCGGTTTCT CAACTCCACA CAGTAATCGT 
GCATACTCCA CTGCCTTGGG ACAGGGTGCC TTCATACAAG CTGAGCTTTC AGTGCAAAGG 
CCAGAGACTG AGATAAATGC TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32b ( XM_021469081.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324756.1 Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ODa25889
Clone ID Related Accession (Same CDS sequence)	XM_005161263.3 , XM_005161263.4 , XM_021469081.1
Accession Version	XM_021469081.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4707bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho GTPase-activating protein 32 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCCA GGCCGACCAA GCAGAAGCTG AAGCAAAGAG GTATCCTCAG GGAGAGGGTG 
TTCGGCTGTG ACCTAGGAGA GCATCTCCTC AACTCTGGCC ATGATGTCCC CCAGGTACTC 
AAGAGCTGCA CCGAGTTCAT TGAGAAGCAT GGAGTGGTTG ATGGAATGTA TCGTCTCTCT 
GGGATCGCCT CCAACATTCA GAAGCTGCGG CACGAGTTTG ATTCTGAACA GATCCCAGAT 
CTGACAAAAG ATGTTTACAT TCAGGATATA CACTGCGTGG GCTCCCTGTG CAAGCTCTAC 
TTTCGAGAGC TTCCCAACCC ACTTCTCACC TACCAGCTGT ACGAGAAATT CTCGGAGGCT 
GTGTCAGCAG CCACAGACGA GGAGAGGCTG ATCAAAATAC ATGACGTCAT TCAGCAGCTG 
CCGCCTCCGC ATTATAGAAC CCTTGAGTTT CTTATGAGGC ATCTTTCACA CTTGGCAACC 
TTCAGCTATG TAACGAACAT GCATACTAAA AATTTGGCCA TCGTTTGGGC CCCCAACTTA 
CTGAGGTCAA AGCAAATAGA ATCTGCTTGC TTCAGTGGGA CAGCAGCCTT CATGGAAGTA 
CGAATACAAT CGGTTGTGGT GGAGTTCATC CTGAATCATG TGGATGTTCT CTTCAGTCCC 
AAACTGAGCT CTCTTATTCG AGAGGGAACA GGACACAACT CTTTGTCTCG GCCTAAGTCT 
TTGCTAGTAC CGTCTCCATC CACAAAGCTG CTAACTCTAG AAGAGGCACA GGCACGCACA 
CAGGCCCAGA TCAACTCACC CATCACTGCA GACAGCAAGT ACATAGAGGT CGGAGAGGGT 
CCTGCAGCAT TACAAGGAAA ATTCCACACA GTCATAGAAT TCCCTACTGA GAGGAAGAGG 
CAGCCCATGA AGTCTAAGAA GTCTCCAGTT GGCAGTTGGC GTTCTTTTTT CAACCTGGGC 
AAGTCTTCTT CAATGTCGAA ACGCAAACTA CAGCGTAATC CCAGTGAACC AAATGAACTC 
AAGGCTATGG CACTGGCTGG AGGAAGAGGA GACACTGGTA CTCTTCGGTC AGCCAAAAGT 
GAAGAGTCTC TCACCTCTCT ACATAATGTT GAAGGAGAGT CTAAAGTGTA TAGGCCCCGC 
AGACCTCGCT CCAGCAGTGA TGCCCTCTCT GCTTCATTCA ATGGTGACCT TCTGGATACC 
AGACAGCACT GTAACTCGTA CGACAACCTA GGTCATGGAG ACAGTGATGG GGATGATGGT 
CCAATCTGTG TGCCTGCTCT CATCTCCCCA CCACGCTCCG CTGATGATGT GGATCTGAGC 
CCCCCTGACA TTGGCATGGC CTCTTTAGAC TTTGACCCCA TGTCTTTCCA ATGTAGCCTT 
CCTCTGGCAG AAACTTCGAT TTTCTCAATT GACGCAGATA CAAGCAGTCT AAAAAGGAGC 
CCTGCCAGTG GCTCAGAGTC AGTCTCGCCA GTCCGAGCTA AAGTTACGAG CCGGTTTTTG 
TCTCCAGACC ACAGTACAGC CTTAAAGGAT AAACATAAAC TGCTCCTTAC ATCTTTCTCT 
GAGAAAACTC CAGTTCAGTC TGTTGAAAGC ACCGAGATGT CAGCAGTTGT TTCTCCTCCA 
AGCATCTCTG AATCAGCTGC CTCCGTGATA ATGAAAGGAC CAGAGAGCAC TGTGCTTCAG 
CCCTCTTCCC CTCCATCTCC ACTCGACTCT CCTACAAAAT TGAGCTCTCT GTCAAGAACC 
ACCGATCTGC CCCTGAGTGA AGCCATTCAA CAAGAGCTTC TTTCTAAAGC TGCTACCTTT 
GAGAGTAAAG ACATATCGAG CATTGAATGT TCACAACCAC CACAGGTCAC CTGCTCTCAA 
GAACAGCCAG GAGCCGTAGC TTTGGACTCT CCAAAGGGCA ATGTCCCTGT CAGCTCTGTG 
TCCCTCGTCC CTCCCCCTCC CCCACCAAAG AACGCTGCCC GCATGCTTGC ACTAGCTCTA 
GCTGAATCTG CCCAGCAGGC CACCATTCTT TCTCAGAAGC AATCCTCTGG ACCCCCTACA 
CCAGTATCCC CTCTTAGGCC TCAAGAGTTA TTGGAGACCC TGGACTGGCC GCTGCCTTCT 
TCCTTGCCAT CACAGCCATC CGAGGAGGAA ACTGCAGTAG AATCATCAAA TAAATCGGTT 
CCTTTGTCTT CACCACCCTC CACTCCACCT GAAAAAATGA CTCGCTCAGT CAGTCTGCAC 
CTCACCACAG CTGATAGTGG GAAGCCATCC AGCATTTCAT GCAACAGCCA GGATGTAATC 
TCACCAGAGA CCCCAAGCCA GAGTGCTATG CCCTCTTGTC TGACTTCTCA TGTTTCTCAA 
ACACAAAAAA GTCCAGAAAA ACAGCAGCCT GCTGTCGGTC AGACACCTGT GAGCACAGCA 
AAAACCACCA CCACAACCAC CACTTCAACC ATCAGCAGTA AGGATGCTGT CATTCAACAA 
CCCACCTCTG AGATCCATAT GCCTGAAACA GTCCTGCCAC CAAAACCAAA CAAGGTGCCA 
GATCAACCAG TGGCAATTCT TCAGCCTCAG CCTCAAATGC AGACGCAACC TCATACTGCA 
TTTCGGTCTC AACCTCAAGT TCAGCCTCTT CCTCAGTCAC AAGCACAGCC ACAGTCTCAG 
CCCAGATCAC AGCCCAATGC TAGAGTTGCA CCAACCCTGG CAGCACCTCA TGATCCTGTG 
GAAAAACCAT GGGAAGCCAT AAAGCCTGTC CATCCAAAAG GAGATAGTGC TTCTCAACAA 
CACCACACTC AGGGAGTGAC ACCACCAACA CCCCCTATTC GCTCGATTGA AAGTAAACTA 
GCAGCAGCTG CACTTTGCAA CACCGAGGCT GCAAACCCTG CCAACTTTCA TGCCATGTTA 
GAGACATCCA TGAGGGGATC AGTAGAGGAG ACTCCCCCAC AGTCTCATCC GCCTCACCGC 
AGATCGTCTA CTCATCAGTC AGGTTATCTT TATCACACAA AAGGGGAGGC AGCCTTACTG 
GACCCCACCA CAGAGGCATA TTACCATCAG CGGGTAACCC CTTCAGGTCA GGCTACAGTG 
GGGTACCATT ACCGGCCAGA GAGTGTTCCA CCTCACATTT CTTACGTGTC AAAATCAGAG 
CCACACATTT CCTACAGAGG TCATGGGGAT GGTCGATACA ATACAATTGG ACCTAGATCC 
TACCACCAGT CTATCAAATC TCGTGGTTCA CTTAGAAGTG AGTACATCTC TCCAAGCTCA 
TTACACCACT CCCATAGCTA CAGCCAAGCA GATGGACCAA CCGTCTACCC AACAATTCGC 
AGGGTCCACT CCTTACATGT GCCTCCCACT GCTATTCGCT CAATGCCAGT CACCAGAACT 
GAGGTACCAC CAGATGATGA GTTATTTTAC TACCAGCGCC CAATCTACCA CTGCAAGTCT 
CACCAACCAT CATCTCAAGT CGACTATCAC GTCACACAAC TGCAGCCTTA TTTTGAAAAT 
GGAAGAGTTC AGTACCGCTA CAGCCCCTAC TCTGGCTCTC ACGTTCTGGA ATCCCCTTTC 
TATGATGTGG ACCATTATGG TACTATCCGC TTAAGGCACG TCCATTCCTT CAGTGGTCGG 
GACAGTGCCC CTCTCCTTCG ATCTGGAGCA AAATCTGGAG GCTACCACTA CCTTTCACGG 
CATGTTATTC CTTTAAAGGA GCATGGCTTC ATAAGCAGGG ACATGCCACC TTACAGTGGT 
TCAAAAGGGA GTATTTACTT TGCATGGGAC CCAGAGGAGG CAGAAAGGCT GCGCATACAC 
TCCATCCGTA GGGAAAGCAG AGCAAGGCAA AAGGTTAAGG GTTCTGTTAT GTCTCAATAC 
GACAATGTAG GACCCTACAT GCCTGTGGAT GTTGACATGT TACATTTGCG CAGCAAATCA 
GATCCTGGAA AAACTGTATT GGTGTCCTCG GAGAGCAAAG AGGCCAGATA CAATGTTGTG 
CCTGGATCTC AGCATGCTCC CAGACACTTG ATCTCTGATC CAGATGTCCT AATGTATATG 
GAAACAGAGA AGCACTGCCA AGGAAATGGA ATGGGGGAGA AAACCACCAA GCAAGGCAGC 
TCTAGGACCT ACTCGTCTAT ACATTCACAT CAATCCCAGC TTCCACCACT AAGCCGGCAG 
CACTTACCAG AGGGTGGCCA TCATGACCCA AAGTTTGAAC CAGGGGAAAA GCAACTGAGT 
GGTAAACATT GGCAGGAACA TTCGGACAGC AGGAGTGCTC AAGCACGTTA TGAAAGGTCT 
GATTTGGATC GCCACATATG CAGGGTGAAG GCCACTAGCA GTGCCAGTGA AGATGAACAA 
GCTGCTCCTG TGAAGCCGGC TCCTCCACCT AAGCCCGAGA GATCCCATAG TGTTCGAGAG 
CGGCACCATT ACAACCAGTC CAGTCTTCCT GCATATTTAC CAGACAATTC AGATAGAGAC 
CACAGTGGAA CATACTCCCA TCAGTCACAG GGGCAGAGAC AAAGCACCGT TACTTTGCAG 
TCGCATTACG ACAACCTGGA TGACTATCAT CCAGTACCTC AATCCCAAAC CACGCTAGCA 
AATCGGGGTT GTTCCAGTTC CTATCATAGC CCCGGTTTCT CAACTCCACA CAGTAATCGT 
GCATACTCCA CTGCCTTGGG ACAGGGTGCC TTCATACAAG CTGAGCTTTC AGTGCAAAGG 
CCAGAGACTG AGATAAATGC TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324756.1
CDS	125..4831
Translation	MKSRPTKQKLKQRGILRERVFGCDLGEHLLNSGHDVPQVLKSCTEFIEKHGVVDGMYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSEAVSAATDEERLIKIHDVIQQLPPPHYRTLEFLMRHLSHLATFSYVTNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVDVLFSPKLSSLIREGTGHNSLSRPKSLLVPSPSTKLLTLEEAQARTQAQINSPITADSKYIEVGEGPAALQGKFHTVIEFPTERKRQPMKSKKSPVGSWRSFFNLGKSSSMSKRKLQRNPSEPNELKAMALAGGRGDTGTLRSAKSEESLTSLHNVEGESKVYRPRRPRSSSDALSASFNGDLLDTRQHCNSYDNLGHGDSDGDDGPICVPALISPPRSADDVDLSPPDIGMASLDFDPMSFQCSLPLAETSIFSIDADTSSLKRSPASGSESVSPVRAKVTSRFLSPDHSTALKDKHKLLLTSFSEKTPVQSVESTEMSAVVSPPSISESAASVIMKGPESTVLQPSSPPSPLDSPTKLSSLSRTTDLPLSEAIQQELLSKAATFESKDISSIECSQPPQVTCSQEQPGAVALDSPKGNVPVSSVSLVPPPPPPKNAARMLALALAESAQQATILSQKQSSGPPTPVSPLRPQELLETLDWPLPSSLPSQPSEEETAVESSNKSVPLSSPPSTPPEKMTRSVSLHLTTADSGKPSSISCNSQDVISPETPSQSAMPSCLTSHVSQTQKSPEKQQPAVGQTPVSTAKTTTTTTTSTISSKDAVIQQPTSEIHMPETVLPPKPNKVPDQPVAILQPQPQMQTQPHTAFRSQPQVQPLPQSQAQPQSQPRSQPNARVAPTLAAPHDPVEKPWEAIKPVHPKGDSASQQHHTQGVTPPTPPIRSIESKLAAAALCNTEAANPANFHAMLETSMRGSVEETPPQSHPPHRRSSTHQSGYLYHTKGEAALLDPTTEAYYHQRVTPSGQATVGYHYRPESVPPHISYVSKSEPHISYRGHGDGRYNTIGPRSYHQSIKSRGSLRSEYISPSSLHHSHSYSQADGPTVYPTIRRVHSLHVPPTAIRSMPVTRTEVPPDDELFYYQRPIYHCKSHQPSSQVDYHVTQLQPYFENGRVQYRYSPYSGSHVLESPFYDVDHYGTIRLRHVHSFSGRDSAPLLRSGAKSGGYHYLSRHVIPLKEHGFISRDMPPYSGSKGSIYFAWDPEEAERLRIHSIRRESRARQKVKGSVMSQYDNVGPYMPVDVDMLHLRSKSDPGKTVLVSSESKEARYNVVPGSQHAPRHLISDPDVLMYMETEKHCQGNGMGEKTTKQGSSRTYSSIHSHQSQLPPLSRQHLPEGGHHDPKFEPGEKQLSGKHWQEHSDSRSAQARYERSDLDRHICRVKATSSASEDEQAAPVKPAPPPKPERSHSVRERHHYNQSSLPAYLPDNSDRDHSGTYSHQSQGQRQSTVTLQSHYDNLDDYHPVPQSQTTLANRGCSSSYHSPGFSTPHSNRAYSTALGQGAFIQAELSVQRPETEINAE

Target ORF information:

RefSeq Version	XM_021469081.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469081.1

ORF Insert Sequence:

ATGAAGTCCA GGCCGACCAA GCAGAAGCTG AAGCAAAGAG GTATCCTCAG GGAGAGGGTG 
TTCGGCTGTG ACCTAGGAGA GCATCTCCTC AACTCTGGCC ATGATGTCCC CCAGGTACTC 
AAGAGCTGCA CCGAGTTCAT TGAGAAGCAT GGAGTGGTTG ATGGAATGTA TCGTCTCTCT 
GGGATCGCCT CCAACATTCA GAAGCTGCGG CACGAGTTTG ATTCTGAACA GATCCCAGAT 
CTGACAAAAG ATGTTTACAT TCAGGATATA CACTGCGTGG GCTCCCTGTG CAAGCTCTAC 
TTTCGAGAGC TTCCCAACCC ACTTCTCACC TACCAGCTGT ACGAGAAATT CTCGGAGGCT 
GTGTCAGCAG CCACAGACGA GGAGAGGCTG ATCAAAATAC ATGACGTCAT TCAGCAGCTG 
CCGCCTCCGC ATTATAGAAC CCTTGAGTTT CTTATGAGGC ATCTTTCACA CTTGGCAACC 
TTCAGCTATG TAACGAACAT GCATACTAAA AATTTGGCCA TCGTTTGGGC CCCCAACTTA 
CTGAGGTCAA AGCAAATAGA ATCTGCTTGC TTCAGTGGGA CAGCAGCCTT CATGGAAGTA 
CGAATACAAT CGGTTGTGGT GGAGTTCATC CTGAATCATG TGGATGTTCT CTTCAGTCCC 
AAACTGAGCT CTCTTATTCG AGAGGGAACA GGACACAACT CTTTGTCTCG GCCTAAGTCT 
TTGCTAGTAC CGTCTCCATC CACAAAGCTG CTAACTCTAG AAGAGGCACA GGCACGCACA 
CAGGCCCAGA TCAACTCACC CATCACTGCA GACAGCAAGT ACATAGAGGT CGGAGAGGGT 
CCTGCAGCAT TACAAGGAAA ATTCCACACA GTCATAGAAT TCCCTACTGA GAGGAAGAGG 
CAGCCCATGA AGTCTAAGAA GTCTCCAGTT GGCAGTTGGC GTTCTTTTTT CAACCTGGGC 
AAGTCTTCTT CAATGTCGAA ACGCAAACTA CAGCGTAATC CCAGTGAACC AAATGAACTC 
AAGGCTATGG CACTGGCTGG AGGAAGAGGA GACACTGGTA CTCTTCGGTC AGCCAAAAGT 
GAAGAGTCTC TCACCTCTCT ACATAATGTT GAAGGAGAGT CTAAAGTGTA TAGGCCCCGC 
AGACCTCGCT CCAGCAGTGA TGCCCTCTCT GCTTCATTCA ATGGTGACCT TCTGGATACC 
AGACAGCACT GTAACTCGTA CGACAACCTA GGTCATGGAG ACAGTGATGG GGATGATGGT 
CCAATCTGTG TGCCTGCTCT CATCTCCCCA CCACGCTCCG CTGATGATGT GGATCTGAGC 
CCCCCTGACA TTGGCATGGC CTCTTTAGAC TTTGACCCCA TGTCTTTCCA ATGTAGCCTT 
CCTCTGGCAG AAACTTCGAT TTTCTCAATT GACGCAGATA CAAGCAGTCT AAAAAGGAGC 
CCTGCCAGTG GCTCAGAGTC AGTCTCGCCA GTCCGAGCTA AAGTTACGAG CCGGTTTTTG 
TCTCCAGACC ACAGTACAGC CTTAAAGGAT AAACATAAAC TGCTCCTTAC ATCTTTCTCT 
GAGAAAACTC CAGTTCAGTC TGTTGAAAGC ACCGAGATGT CAGCAGTTGT TTCTCCTCCA 
AGCATCTCTG AATCAGCTGC CTCCGTGATA ATGAAAGGAC CAGAGAGCAC TGTGCTTCAG 
CCCTCTTCCC CTCCATCTCC ACTCGACTCT CCTACAAAAT TGAGCTCTCT GTCAAGAACC 
ACCGATCTGC CCCTGAGTGA AGCCATTCAA CAAGAGCTTC TTTCTAAAGC TGCTACCTTT 
GAGAGTAAAG ACATATCGAG CATTGAATGT TCACAACCAC CACAGGTCAC CTGCTCTCAA 
GAACAGCCAG GAGCCGTAGC TTTGGACTCT CCAAAGGGCA ATGTCCCTGT CAGCTCTGTG 
TCCCTCGTCC CTCCCCCTCC CCCACCAAAG AACGCTGCCC GCATGCTTGC ACTAGCTCTA 
GCTGAATCTG CCCAGCAGGC CACCATTCTT TCTCAGAAGC AATCCTCTGG ACCCCCTACA 
CCAGTATCCC CTCTTAGGCC TCAAGAGTTA TTGGAGACCC TGGACTGGCC GCTGCCTTCT 
TCCTTGCCAT CACAGCCATC CGAGGAGGAA ACTGCAGTAG AATCATCAAA TAAATCGGTT 
CCTTTGTCTT CACCACCCTC CACTCCACCT GAAAAAATGA CTCGCTCAGT CAGTCTGCAC 
CTCACCACAG CTGATAGTGG GAAGCCATCC AGCATTTCAT GCAACAGCCA GGATGTAATC 
TCACCAGAGA CCCCAAGCCA GAGTGCTATG CCCTCTTGTC TGACTTCTCA TGTTTCTCAA 
ACACAAAAAA GTCCAGAAAA ACAGCAGCCT GCTGTCGGTC AGACACCTGT GAGCACAGCA 
AAAACCACCA CCACAACCAC CACTTCAACC ATCAGCAGTA AGGATGCTGT CATTCAACAA 
CCCACCTCTG AGATCCATAT GCCTGAAACA GTCCTGCCAC CAAAACCAAA CAAGGTGCCA 
GATCAACCAG TGGCAATTCT TCAGCCTCAG CCTCAAATGC AGACGCAACC TCATACTGCA 
TTTCGGTCTC AACCTCAAGT TCAGCCTCTT CCTCAGTCAC AAGCACAGCC ACAGTCTCAG 
CCCAGATCAC AGCCCAATGC TAGAGTTGCA CCAACCCTGG CAGCACCTCA TGATCCTGTG 
GAAAAACCAT GGGAAGCCAT AAAGCCTGTC CATCCAAAAG GAGATAGTGC TTCTCAACAA 
CACCACACTC AGGGAGTGAC ACCACCAACA CCCCCTATTC GCTCGATTGA AAGTAAACTA 
GCAGCAGCTG CACTTTGCAA CACCGAGGCT GCAAACCCTG CCAACTTTCA TGCCATGTTA 
GAGACATCCA TGAGGGGATC AGTAGAGGAG ACTCCCCCAC AGTCTCATCC GCCTCACCGC 
AGATCGTCTA CTCATCAGTC AGGTTATCTT TATCACACAA AAGGGGAGGC AGCCTTACTG 
GACCCCACCA CAGAGGCATA TTACCATCAG CGGGTAACCC CTTCAGGTCA GGCTACAGTG 
GGGTACCATT ACCGGCCAGA GAGTGTTCCA CCTCACATTT CTTACGTGTC AAAATCAGAG 
CCACACATTT CCTACAGAGG TCATGGGGAT GGTCGATACA ATACAATTGG ACCTAGATCC 
TACCACCAGT CTATCAAATC TCGTGGTTCA CTTAGAAGTG AGTACATCTC TCCAAGCTCA 
TTACACCACT CCCATAGCTA CAGCCAAGCA GATGGACCAA CCGTCTACCC AACAATTCGC 
AGGGTCCACT CCTTACATGT GCCTCCCACT GCTATTCGCT CAATGCCAGT CACCAGAACT 
GAGGTACCAC CAGATGATGA GTTATTTTAC TACCAGCGCC CAATCTACCA CTGCAAGTCT 
CACCAACCAT CATCTCAAGT CGACTATCAC GTCACACAAC TGCAGCCTTA TTTTGAAAAT 
GGAAGAGTTC AGTACCGCTA CAGCCCCTAC TCTGGCTCTC ACGTTCTGGA ATCCCCTTTC 
TATGATGTGG ACCATTATGG TACTATCCGC TTAAGGCACG TCCATTCCTT CAGTGGTCGG 
GACAGTGCCC CTCTCCTTCG ATCTGGAGCA AAATCTGGAG GCTACCACTA CCTTTCACGG 
CATGTTATTC CTTTAAAGGA GCATGGCTTC ATAAGCAGGG ACATGCCACC TTACAGTGGT 
TCAAAAGGGA GTATTTACTT TGCATGGGAC CCAGAGGAGG CAGAAAGGCT GCGCATACAC 
TCCATCCGTA GGGAAAGCAG AGCAAGGCAA AAGGTTAAGG GTTCTGTTAT GTCTCAATAC 
GACAATGTAG GACCCTACAT GCCTGTGGAT GTTGACATGT TACATTTGCG CAGCAAATCA 
GATCCTGGAA AAACTGTATT GGTGTCCTCG GAGAGCAAAG AGGCCAGATA CAATGTTGTG 
CCTGGATCTC AGCATGCTCC CAGACACTTG ATCTCTGATC CAGATGTCCT AATGTATATG 
GAAACAGAGA AGCACTGCCA AGGAAATGGA ATGGGGGAGA AAACCACCAA GCAAGGCAGC 
TCTAGGACCT ACTCGTCTAT ACATTCACAT CAATCCCAGC TTCCACCACT AAGCCGGCAG 
CACTTACCAG AGGGTGGCCA TCATGACCCA AAGTTTGAAC CAGGGGAAAA GCAACTGAGT 
GGTAAACATT GGCAGGAACA TTCGGACAGC AGGAGTGCTC AAGCACGTTA TGAAAGGTCT 
GATTTGGATC GCCACATATG CAGGGTGAAG GCCACTAGCA GTGCCAGTGA AGATGAACAA 
GCTGCTCCTG TGAAGCCGGC TCCTCCACCT AAGCCCGAGA GATCCCATAG TGTTCGAGAG 
CGGCACCATT ACAACCAGTC CAGTCTTCCT GCATATTTAC CAGACAATTC AGATAGAGAC 
CACAGTGGAA CATACTCCCA TCAGTCACAG GGGCAGAGAC AAAGCACCGT TACTTTGCAG 
TCGCATTACG ACAACCTGGA TGACTATCAT CCAGTACCTC AATCCCAAAC CACGCTAGCA 
AATCGGGGTT GTTCCAGTTC CTATCATAGC CCCGGTTTCT CAACTCCACA CAGTAATCGT 
GCATACTCCA CTGCCTTGGG ACAGGGTGCC TTCATACAAG CTGAGCTTTC AGTGCAAAGG 
CCAGAGACTG AGATAAATGC TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32b ( XM_009295410.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293685.1 Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X1, mRNA.

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CloneID	ODa45739
Clone ID Related Accession (Same CDS sequence)	XM_009295410.3 , XM_009295412.3 , XM_009295411.2 , XM_009295412.2 , XM_009295410.2 , XM_009295411.3
Accession Version	XM_009295410.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho GTPase-activating protein 32 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295410.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGT CTCTTTTCTC CCCTCAGGGG CCTGAGGCCT 
GGACGGCACC CTGAAGAAGA CGATATCGTG CCAGAGCTTG CCAGGAGCAT CCACCCCCGA 
GAGCGACCTG ACTGGGAGGA AACCATCAGC GCAATGGCAC GAAGTGCTGA AATTCCAGAG 
CTGAGCTCTG AGCCGCTCAT GCGCTCCTGC AGCAGCACCG CCAGTATGAA GGTGAAGAAT 
GTCAAAAAGC TGTCATTCAC CAAGGGCCAT TTTCCAAAGT TAGCTGAATG TGCCCACTTC 
CACTATGAAA ACGTTGATTT TGGAACAATA CAGTTAACCC TCGCAGATGA GCAGACTGAA 
GTGACACGGA ATGGCCTGGA GTCAAAAGAG CCTGTGTATT TGGTTCAGAT CTACTGTCAG 
GGACGAAGCT GGATCGTGAG ACGAAGCTAT GAAGATTTCC GAGTCTTGGA TAAGCATCTC 
CATCTCTGCA TCTATGACCG CCGATTTTCC CAGCTCCCTG AGCTGCCCCG TTTCGACAGC 
CTGAGGGAGA GAGCGGAGGC AGTTTCCCAG ATGCTGATGG CTTACCTCTC CCGTCTCTCA 
GCCATTGCTG ATAACAAGAT CAATTGTGGG CCGGCCCTCA CGTGGATGGA GGTTGACAAC 
AAAGGGAATC ATTTGCTGGT GCATGAAGAA TCATCTATAA ATGTTCCAGC TATCGCAGCG 
GCCCACGTGA TCAAGCGCTA CATCGCACAG GCAGCTGATG AGCTGTCATT TGAGGTGGGG 
GATATTGTTT CTGTCATTGA CATGCCACCC AAAGAGGACA CCACCTGGTG GAGGGGAAAA 
CATGGTTTCC AGGTTGGCTT CTTTCCCAGC GAGTGTGTGG AGCTGATAAA CGACAAGGTC 
CCGCAGTCTG TCACCAACTC TGTGCCAAAA CCAGCGTCCC CCTGCTCTGG TCTGCGGCCG 
GCATCCTGGA GTCTGTTCCC ACCCTATTTG GAGATGGAGA GTATAAAGCA GGACAGTGCA 
TTGGCACCCG ACCCATTAAA CCCCTACAGG CTGAGCTCAG TATCCAAGAA ACACGGGAAA 
CTGATCACTT TTCTGCGCAC ATTCATGAAG TCCAGGCCGA CCAAGCAGAA GCTGAAGCAA 
AGAGGTATCC TCAGGGAGAG GGTGTTCGGC TGTGACCTAG GAGAGCATCT CCTCAACTCT 
GGCCATGATG TCCCCCAGGT ACTCAAGAGC TGCACCGAGT TCATTGAGAA GCATGGAGTG 
GTTGATGGAA TGTATCGTCT CTCTGGGATC GCCTCCAACA TTCAGAAGCT GCGGCACGAG 
TTTGATTCTG AACAGATCCC AGATCTGACA AAAGATGTTT ACATTCAGGA TATACACTGC 
GTGGGCTCCC TGTGCAAGCT CTACTTTCGA GAGCTTCCCA ACCCACTTCT CACCTACCAG 
CTGTACGAGA AATTCTCGGA GGCTGTGTCA GCAGCCACAG ACGAGGAGAG GCTGATCAAA 
ATACATGACG TCATTCAGCA GCTGCCGCCT CCGCATTATA GAACCCTTGA GTTTCTTATG 
AGGCATCTTT CACACTTGGC AACCTTCAGC TATGTAACGA ACATGCATAC TAAAAATTTG 
GCCATCGTTT GGGCCCCCAA CTTACTGAGG TCAAAGCAAA TAGAATCTGC TTGCTTCAGT 
GGGACAGCAG CCTTCATGGA AGTACGAATA CAATCGGTTG TGGTGGAGTT CATCCTGAAT 
CATGTGGATG TTCTCTTCAG TCCCAAACTG AGCTCTCTTA TTCGAGAGGG AACAGGACAC 
AACTCTTTGT CTCGGCCTAA GTCTTTGCTA GTACCGTCTC CATCCACAAA GCTGCTAACT 
CTAGAAGAGG CACAGGCACG CACACAGGCC CAGATCAACT CACCCATCAC TGCAGACAGC 
AAGTACATAG AGGTCGGAGA GGGTCCTGCA GCATTACAAG GAAAATTCCA CACAGTCATA 
GAATTCCCTA CTGAGAGGAA GAGGCAGCCC ATGAAGTCTA AGAAGTCTCC AGTTGGCAGT 
TGGCGTTCTT TTTTCAACCT GGGCAAGTCT TCTTCAATGT CGAAACGCAA ACTACAGCGT 
AATCCCAGTG AACCAAATGA ACTCAAGGCT ATGGCACTGG CTGGAGGAAG AGGAGACACT 
GGTACTCTTC GGTCAGCCAA AAGTGAAGAG TCTCTCACCT CTCTACATAA TGTTGAAGGA 
GAGTCTAAAG TGTATAGGCC CCGCAGACCT CGCTCCAGCA GTGATGCCCT CTCTGCTTCA 
TTCAATGGTG ACCTTCTGGA TACCAGACAG CACTGTAACT CGTACGACAA CCTAGGTCAT 
GGAGACAGTG ATGGGGATGA TGGTCCAATC TGTGTGCCTG CTCTCATCTC CCCACCACGC 
TCCGCTGATG ATGTGGATCT GAGCCCCCCT GACATTGGCA TGGCCTCTTT AGACTTTGAC 
CCCATGTCTT TCCAATGTAG CCTTCCTCTG GCAGAAACTT CGATTTTCTC AATTGACGCA 
GATACAAGCA GTCTAAAAAG GAGCCCTGCC AGTGGCTCAG AGTCAGTCTC GCCAGTCCGA 
GCTAAAGTTA CGAGCCGGTT TTTGTCTCCA GACCACAGTA CAGCCTTAAA GGATAAACAT 
AAACTGCTCC TTACATCTTT CTCTGAGAAA ACTCCAGTTC AGTCTGTTGA AAGCACCGAG 
ATGTCAGCAG TTGTTTCTCC TCCAAGCATC TCTGAATCAG CTGCCTCCGT GATAATGAAA 
GGACCAGAGA GCACTGTGCT TCAGCCCTCT TCCCCTCCAT CTCCACTCGA CTCTCCTACA 
AAATTGAGCT CTCTGTCAAG AACCACCGAT CTGCCCCTGA GTGAAGCCAT TCAACAAGAG 
CTTCTTTCTA AAGCTGCTAC CTTTGAGAGT AAAGACATAT CGAGCATTGA ATGTTCACAA 
CCACCACAGG TCACCTGCTC TCAAGAACAG CCAGGAGCCG TAGCTTTGGA CTCTCCAAAG 
GGCAATGTCC CTGTCAGCTC TGTGTCCCTC GTCCCTCCCC CTCCCCCACC AAAGAACGCT 
GCCCGCATGC TTGCACTAGC TCTAGCTGAA TCTGCCCAGC AGGCCACCAT TCTTTCTCAG 
AAGCAATCCT CTGGACCCCC TACACCAGTA TCCCCTCTTA GGCCTCAAGA GTTATTGGAG 
ACCCTGGACT GGCCGCTGCC TTCTTCCTTG CCATCACAGC CATCCGAGGA GGAAACTGCA 
GTAGAATCAT CAAATAAATC GGTTCCTTTG TCTTCACCAC CCTCCACTCC ACCTGAAAAA 
ATGACTCGCT CAGTCAGTCT GCACCTCACC ACAGCTGATA GTGGGAAGCC ATCCAGCATT 
TCATGCAACA GCCAGGATGT AATCTCACCA GAGACCCCAA GCCAGAGTGC TATGCCCTCT 
TGTCTGACTT CTCATGTTTC TCAAACACAA AAAAGTCCAG AAAAACAGCA GCCTGCTGTC 
GGTCAGACAC CTGTGAGCAC AGCAAAAACC ACCACCACAA CCACCACTTC AACCATCAGC 
AGTAAGGATG CTGTCATTCA ACAACCCACC TCTGAGATCC ATATGCCTGA AACAGTCCTG 
CCACCAAAAC CAAACAAGGT GCCAGATCAA CCAGTGGCAA TTCTTCAGCC TCAGCCTCAA 
ATGCAGACGC AACCTCATAC TGCATTTCGG TCTCAACCTC AAGTTCAGCC TCTTCCTCAG 
TCACAAGCAC AGCCACAGTC TCAGCCCAGA TCACAGCCCA ATGCTAGAGT TGCACCAACC 
CTGGCAGCAC CTCATGATCC TGTGGAAAAA CCATGGGAAG CCATAAAGCC TGTCCATCCA 
AAAGGAGATA GTGCTTCTCA ACAACACCAC ACTCAGGGAG TGACACCACC AACACCCCCT 
ATTCGCTCGA TTGAAAGTAA ACTAGCAGCA GCTGCACTTT GCAACACCGA GGCTGCAAAC 
CCTGCCAACT TTCATGCCAT GTTAGAGACA TCCATGAGGG GATCAGTAGA GGAGACTCCC 
CCACAGTCTC ATCCGCCTCA CCGCAGATCG TCTACTCATC AGTCAGGTTA TCTTTATCAC 
ACAAAAGGGG AGGCAGCCTT ACTGGACCCC ACCACAGAGG CATATTACCA TCAGCGGGTA 
ACCCCTTCAG GTCAGGCTAC AGTGGGGTAC CATTACCGGC CAGAGAGTGT TCCACCTCAC 
ATTTCTTACG TGTCAAAATC AGAGCCACAC ATTTCCTACA GAGGTCATGG GGATGGTCGA 
TACAATACAA TTGGACCTAG ATCCTACCAC CAGTCTATCA AATCTCGTGG TTCACTTAGA 
AGTGAGTACA TCTCTCCAAG CTCATTACAC CACTCCCATA GCTACAGCCA AGCAGATGGA 
CCAACCGTCT ACCCAACAAT TCGCAGGGTC CACTCCTTAC ATGTGCCTCC CACTGCTATT 
CGCTCAATGC CAGTCACCAG AACTGAGGTA CCACCAGATG ATGAGTTATT TTACTACCAG 
CGCCCAATCT ACCACTGCAA GTCTCACCAA CCATCATCTC AAGTCGACTA TCACGTCACA 
CAACTGCAGC CTTATTTTGA AAATGGAAGA GTTCAGTACC GCTACAGCCC CTACTCTGGC 
TCTCACGTTC TGGAATCCCC TTTCTATGAT GTGGACCATT ATGGTACTAT CCGCTTAAGG 
CACGTCCATT CCTTCAGTGG TCGGGACAGT GCCCCTCTCC TTCGATCTGG AGCAAAATCT 
GGAGGCTACC ACTACCTTTC ACGGCATGTT ATTCCTTTAA AGGAGCATGG CTTCATAAGC 
AGGGACATGC CACCTTACAG TGGTTCAAAA GGGAGTATTT ACTTTGCATG GGACCCAGAG 
GAGGCAGAAA GGCTGCGCAT ACACTCCATC CGTAGGGAAA GCAGAGCAAG GCAAAAGGTT 
AAGGGTTCTG TTATGTCTCA ATACGACAAT GTAGGACCCT ACATGCCTGT GGATGTTGAC 
ATGTTACATT TGCGCAGCAA ATCAGATCCT GGAAAAACTG TATTGGTGTC CTCGGAGAGC 
AAAGAGGCCA GATACAATGT TGTGCCTGGA TCTCAGCATG CTCCCAGACA CTTGATCTCT 
GATCCAGATG TCCTAATGTA TATGGAAACA GAGAAGCACT GCCAAGGAAA TGGAATGGGG 
GAGAAAACCA CCAAGCAAGG CAGCTCTAGG ACCTACTCGT CTATACATTC ACATCAATCC 
CAGCTTCCAC CACTAAGCCG GCAGCACTTA CCAGAGGGTG GCCATCATGA CCCAAAGTTT 
GAACCAGGGG AAAAGCAACT GAGTGGTAAA CATTGGCAGG AACATTCGGA CAGCAGGAGT 
GCTCAAGCAC GTTATGAAAG GTCTGATTTG GATCGCCACA TATGCAGGGT GAAGGCCACT 
AGCAGTGCCA GTGAAGATGA ACAAGCTGCT CCTGTGAAGC CGGCTCCTCC ACCTAAGCCC 
GAGAGATCCC ATAGTGTTCG AGAGCGGCAC CATTACAACC AGTCCAGTCT TCCTGCATAT 
TTACCAGACA ATTCAGATAG AGACCACAGT GGAACATACT CCCATCAGTC ACAGGGGCAG 
AGACAAAGCA CCGTTACTTT GCAGTCGCAT TACGACAACC TGGATGACTA TCATCCAGTA 
CCTCAATCCC AAACCACGCT AGCAAATCGG GGTTGTTCCA GTTCCTATCA TAGCCCCGGT 
TTCTCAACTC CACACAGTAA TCGTGCATAC TCCACTGCCT TGGGACAGGG TGCCTTCATA 
CAAGCTGAGC TTTCAGTGCA AAGGCCAGAG ACTGAGATAA ATGCTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293685.1
CDS	521..6391
Translation	MEAGSGAAAVVGSAALGLLGATGSHDISDSLFSPLRGLRPGRHPEEDDIVPELARSIHPRERPDWEETISAMARSAEIPELSSEPLMRSCSSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGTIQLTLADEQTEVTRNGLESKEPVYLVQIYCQGRSWIVRRSYEDFRVLDKHLHLCIYDRRFSQLPELPRFDSLRERAEAVSQMLMAYLSRLSAIADNKINCGPALTWMEVDNKGNHLLVHEESSINVPAIAAAHVIKRYIAQAADELSFEVGDIVSVIDMPPKEDTTWWRGKHGFQVGFFPSECVELINDKVPQSVTNSVPKPASPCSGLRPASWSLFPPYLEMESIKQDSALAPDPLNPYRLSSVSKKHGKLITFLRTFMKSRPTKQKLKQRGILRERVFGCDLGEHLLNSGHDVPQVLKSCTEFIEKHGVVDGMYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSEAVSAATDEERLIKIHDVIQQLPPPHYRTLEFLMRHLSHLATFSYVTNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVDVLFSPKLSSLIREGTGHNSLSRPKSLLVPSPSTKLLTLEEAQARTQAQINSPITADSKYIEVGEGPAALQGKFHTVIEFPTERKRQPMKSKKSPVGSWRSFFNLGKSSSMSKRKLQRNPSEPNELKAMALAGGRGDTGTLRSAKSEESLTSLHNVEGESKVYRPRRPRSSSDALSASFNGDLLDTRQHCNSYDNLGHGDSDGDDGPICVPALISPPRSADDVDLSPPDIGMASLDFDPMSFQCSLPLAETSIFSIDADTSSLKRSPASGSESVSPVRAKVTSRFLSPDHSTALKDKHKLLLTSFSEKTPVQSVESTEMSAVVSPPSISESAASVIMKGPESTVLQPSSPPSPLDSPTKLSSLSRTTDLPLSEAIQQELLSKAATFESKDISSIECSQPPQVTCSQEQPGAVALDSPKGNVPVSSVSLVPPPPPPKNAARMLALALAESAQQATILSQKQSSGPPTPVSPLRPQELLETLDWPLPSSLPSQPSEEETAVESSNKSVPLSSPPSTPPEKMTRSVSLHLTTADSGKPSSISCNSQDVISPETPSQSAMPSCLTSHVSQTQKSPEKQQPAVGQTPVSTAKTTTTTTTSTISSKDAVIQQPTSEIHMPETVLPPKPNKVPDQPVAILQPQPQMQTQPHTAFRSQPQVQPLPQSQAQPQSQPRSQPNARVAPTLAAPHDPVEKPWEAIKPVHPKGDSASQQHHTQGVTPPTPPIRSIESKLAAAALCNTEAANPANFHAMLETSMRGSVEETPPQSHPPHRRSSTHQSGYLYHTKGEAALLDPTTEAYYHQRVTPSGQATVGYHYRPESVPPHISYVSKSEPHISYRGHGDGRYNTIGPRSYHQSIKSRGSLRSEYISPSSLHHSHSYSQADGPTVYPTIRRVHSLHVPPTAIRSMPVTRTEVPPDDELFYYQRPIYHCKSHQPSSQVDYHVTQLQPYFENGRVQYRYSPYSGSHVLESPFYDVDHYGTIRLRHVHSFSGRDSAPLLRSGAKSGGYHYLSRHVIPLKEHGFISRDMPPYSGSKGSIYFAWDPEEAERLRIHSIRRESRARQKVKGSVMSQYDNVGPYMPVDVDMLHLRSKSDPGKTVLVSSESKEARYNVVPGSQHAPRHLISDPDVLMYMETEKHCQGNGMGEKTTKQGSSRTYSSIHSHQSQLPPLSRQHLPEGGHHDPKFEPGEKQLSGKHWQEHSDSRSAQARYERSDLDRHICRVKATSSASEDEQAAPVKPAPPPKPERSHSVRERHHYNQSSLPAYLPDNSDRDHSGTYSHQSQGQRQSTVTLQSHYDNLDDYHPVPQSQTTLANRGCSSSYHSPGFSTPHSNRAYSTALGQGAFIQAELSVQRPETEINAE

Target ORF information:

RefSeq Version	XM_009295410.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295410.3

ORF Insert Sequence:

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGT CTCTTTTCTC CCCTCAGGGG CCTGAGGCCT 
GGACGGCACC CTGAAGAAGA CGATATCGTG CCAGAGCTTG CCAGGAGCAT CCACCCCCGA 
GAGCGACCTG ACTGGGAGGA AACCATCAGC GCAATGGCAC GAAGTGCTGA AATTCCAGAG 
CTGAGCTCTG AGCCGCTCAT GCGCTCCTGC AGCAGCACCG CCAGTATGAA GGTGAAGAAT 
GTCAAAAAGC TGTCATTCAC CAAGGGCCAT TTTCCAAAGT TAGCTGAATG TGCCCACTTC 
CACTATGAAA ACGTTGATTT TGGAACAATA CAGTTAACCC TCGCAGATGA GCAGACTGAA 
GTGACACGGA ATGGCCTGGA GTCAAAAGAG CCTGTGTATT TGGTTCAGAT CTACTGTCAG 
GGACGAAGCT GGATCGTGAG ACGAAGCTAT GAAGATTTCC GAGTCTTGGA TAAGCATCTC 
CATCTCTGCA TCTATGACCG CCGATTTTCC CAGCTCCCTG AGCTGCCCCG TTTCGACAGC 
CTGAGGGAGA GAGCGGAGGC AGTTTCCCAG ATGCTGATGG CTTACCTCTC CCGTCTCTCA 
GCCATTGCTG ATAACAAGAT CAATTGTGGG CCGGCCCTCA CGTGGATGGA GGTTGACAAC 
AAAGGGAATC ATTTGCTGGT GCATGAAGAA TCATCTATAA ATGTTCCAGC TATCGCAGCG 
GCCCACGTGA TCAAGCGCTA CATCGCACAG GCAGCTGATG AGCTGTCATT TGAGGTGGGG 
GATATTGTTT CTGTCATTGA CATGCCACCC AAAGAGGACA CCACCTGGTG GAGGGGAAAA 
CATGGTTTCC AGGTTGGCTT CTTTCCCAGC GAGTGTGTGG AGCTGATAAA CGACAAGGTC 
CCGCAGTCTG TCACCAACTC TGTGCCAAAA CCAGCGTCCC CCTGCTCTGG TCTGCGGCCG 
GCATCCTGGA GTCTGTTCCC ACCCTATTTG GAGATGGAGA GTATAAAGCA GGACAGTGCA 
TTGGCACCCG ACCCATTAAA CCCCTACAGG CTGAGCTCAG TATCCAAGAA ACACGGGAAA 
CTGATCACTT TTCTGCGCAC ATTCATGAAG TCCAGGCCGA CCAAGCAGAA GCTGAAGCAA 
AGAGGTATCC TCAGGGAGAG GGTGTTCGGC TGTGACCTAG GAGAGCATCT CCTCAACTCT 
GGCCATGATG TCCCCCAGGT ACTCAAGAGC TGCACCGAGT TCATTGAGAA GCATGGAGTG 
GTTGATGGAA TGTATCGTCT CTCTGGGATC GCCTCCAACA TTCAGAAGCT GCGGCACGAG 
TTTGATTCTG AACAGATCCC AGATCTGACA AAAGATGTTT ACATTCAGGA TATACACTGC 
GTGGGCTCCC TGTGCAAGCT CTACTTTCGA GAGCTTCCCA ACCCACTTCT CACCTACCAG 
CTGTACGAGA AATTCTCGGA GGCTGTGTCA GCAGCCACAG ACGAGGAGAG GCTGATCAAA 
ATACATGACG TCATTCAGCA GCTGCCGCCT CCGCATTATA GAACCCTTGA GTTTCTTATG 
AGGCATCTTT CACACTTGGC AACCTTCAGC TATGTAACGA ACATGCATAC TAAAAATTTG 
GCCATCGTTT GGGCCCCCAA CTTACTGAGG TCAAAGCAAA TAGAATCTGC TTGCTTCAGT 
GGGACAGCAG CCTTCATGGA AGTACGAATA CAATCGGTTG TGGTGGAGTT CATCCTGAAT 
CATGTGGATG TTCTCTTCAG TCCCAAACTG AGCTCTCTTA TTCGAGAGGG AACAGGACAC 
AACTCTTTGT CTCGGCCTAA GTCTTTGCTA GTACCGTCTC CATCCACAAA GCTGCTAACT 
CTAGAAGAGG CACAGGCACG CACACAGGCC CAGATCAACT CACCCATCAC TGCAGACAGC 
AAGTACATAG AGGTCGGAGA GGGTCCTGCA GCATTACAAG GAAAATTCCA CACAGTCATA 
GAATTCCCTA CTGAGAGGAA GAGGCAGCCC ATGAAGTCTA AGAAGTCTCC AGTTGGCAGT 
TGGCGTTCTT TTTTCAACCT GGGCAAGTCT TCTTCAATGT CGAAACGCAA ACTACAGCGT 
AATCCCAGTG AACCAAATGA ACTCAAGGCT ATGGCACTGG CTGGAGGAAG AGGAGACACT 
GGTACTCTTC GGTCAGCCAA AAGTGAAGAG TCTCTCACCT CTCTACATAA TGTTGAAGGA 
GAGTCTAAAG TGTATAGGCC CCGCAGACCT CGCTCCAGCA GTGATGCCCT CTCTGCTTCA 
TTCAATGGTG ACCTTCTGGA TACCAGACAG CACTGTAACT CGTACGACAA CCTAGGTCAT 
GGAGACAGTG ATGGGGATGA TGGTCCAATC TGTGTGCCTG CTCTCATCTC CCCACCACGC 
TCCGCTGATG ATGTGGATCT GAGCCCCCCT GACATTGGCA TGGCCTCTTT AGACTTTGAC 
CCCATGTCTT TCCAATGTAG CCTTCCTCTG GCAGAAACTT CGATTTTCTC AATTGACGCA 
GATACAAGCA GTCTAAAAAG GAGCCCTGCC AGTGGCTCAG AGTCAGTCTC GCCAGTCCGA 
GCTAAAGTTA CGAGCCGGTT TTTGTCTCCA GACCACAGTA CAGCCTTAAA GGATAAACAT 
AAACTGCTCC TTACATCTTT CTCTGAGAAA ACTCCAGTTC AGTCTGTTGA AAGCACCGAG 
ATGTCAGCAG TTGTTTCTCC TCCAAGCATC TCTGAATCAG CTGCCTCCGT GATAATGAAA 
GGACCAGAGA GCACTGTGCT TCAGCCCTCT TCCCCTCCAT CTCCACTCGA CTCTCCTACA 
AAATTGAGCT CTCTGTCAAG AACCACCGAT CTGCCCCTGA GTGAAGCCAT TCAACAAGAG 
CTTCTTTCTA AAGCTGCTAC CTTTGAGAGT AAAGACATAT CGAGCATTGA ATGTTCACAA 
CCACCACAGG TCACCTGCTC TCAAGAACAG CCAGGAGCCG TAGCTTTGGA CTCTCCAAAG 
GGCAATGTCC CTGTCAGCTC TGTGTCCCTC GTCCCTCCCC CTCCCCCACC AAAGAACGCT 
GCCCGCATGC TTGCACTAGC TCTAGCTGAA TCTGCCCAGC AGGCCACCAT TCTTTCTCAG 
AAGCAATCCT CTGGACCCCC TACACCAGTA TCCCCTCTTA GGCCTCAAGA GTTATTGGAG 
ACCCTGGACT GGCCGCTGCC TTCTTCCTTG CCATCACAGC CATCCGAGGA GGAAACTGCA 
GTAGAATCAT CAAATAAATC GGTTCCTTTG TCTTCACCAC CCTCCACTCC ACCTGAAAAA 
ATGACTCGCT CAGTCAGTCT GCACCTCACC ACAGCTGATA GTGGGAAGCC ATCCAGCATT 
TCATGCAACA GCCAGGATGT AATCTCACCA GAGACCCCAA GCCAGAGTGC TATGCCCTCT 
TGTCTGACTT CTCATGTTTC TCAAACACAA AAAAGTCCAG AAAAACAGCA GCCTGCTGTC 
GGTCAGACAC CTGTGAGCAC AGCAAAAACC ACCACCACAA CCACCACTTC AACCATCAGC 
AGTAAGGATG CTGTCATTCA ACAACCCACC TCTGAGATCC ATATGCCTGA AACAGTCCTG 
CCACCAAAAC CAAACAAGGT GCCAGATCAA CCAGTGGCAA TTCTTCAGCC TCAGCCTCAA 
ATGCAGACGC AACCTCATAC TGCATTTCGG TCTCAACCTC AAGTTCAGCC TCTTCCTCAG 
TCACAAGCAC AGCCACAGTC TCAGCCCAGA TCACAGCCCA ATGCTAGAGT TGCACCAACC 
CTGGCAGCAC CTCATGATCC TGTGGAAAAA CCATGGGAAG CCATAAAGCC TGTCCATCCA 
AAAGGAGATA GTGCTTCTCA ACAACACCAC ACTCAGGGAG TGACACCACC AACACCCCCT 
ATTCGCTCGA TTGAAAGTAA ACTAGCAGCA GCTGCACTTT GCAACACCGA GGCTGCAAAC 
CCTGCCAACT TTCATGCCAT GTTAGAGACA TCCATGAGGG GATCAGTAGA GGAGACTCCC 
CCACAGTCTC ATCCGCCTCA CCGCAGATCG TCTACTCATC AGTCAGGTTA TCTTTATCAC 
ACAAAAGGGG AGGCAGCCTT ACTGGACCCC ACCACAGAGG CATATTACCA TCAGCGGGTA 
ACCCCTTCAG GTCAGGCTAC AGTGGGGTAC CATTACCGGC CAGAGAGTGT TCCACCTCAC 
ATTTCTTACG TGTCAAAATC AGAGCCACAC ATTTCCTACA GAGGTCATGG GGATGGTCGA 
TACAATACAA TTGGACCTAG ATCCTACCAC CAGTCTATCA AATCTCGTGG TTCACTTAGA 
AGTGAGTACA TCTCTCCAAG CTCATTACAC CACTCCCATA GCTACAGCCA AGCAGATGGA 
CCAACCGTCT ACCCAACAAT TCGCAGGGTC CACTCCTTAC ATGTGCCTCC CACTGCTATT 
CGCTCAATGC CAGTCACCAG AACTGAGGTA CCACCAGATG ATGAGTTATT TTACTACCAG 
CGCCCAATCT ACCACTGCAA GTCTCACCAA CCATCATCTC AAGTCGACTA TCACGTCACA 
CAACTGCAGC CTTATTTTGA AAATGGAAGA GTTCAGTACC GCTACAGCCC CTACTCTGGC 
TCTCACGTTC TGGAATCCCC TTTCTATGAT GTGGACCATT ATGGTACTAT CCGCTTAAGG 
CACGTCCATT CCTTCAGTGG TCGGGACAGT GCCCCTCTCC TTCGATCTGG AGCAAAATCT 
GGAGGCTACC ACTACCTTTC ACGGCATGTT ATTCCTTTAA AGGAGCATGG CTTCATAAGC 
AGGGACATGC CACCTTACAG TGGTTCAAAA GGGAGTATTT ACTTTGCATG GGACCCAGAG 
GAGGCAGAAA GGCTGCGCAT ACACTCCATC CGTAGGGAAA GCAGAGCAAG GCAAAAGGTT 
AAGGGTTCTG TTATGTCTCA ATACGACAAT GTAGGACCCT ACATGCCTGT GGATGTTGAC 
ATGTTACATT TGCGCAGCAA ATCAGATCCT GGAAAAACTG TATTGGTGTC CTCGGAGAGC 
AAAGAGGCCA GATACAATGT TGTGCCTGGA TCTCAGCATG CTCCCAGACA CTTGATCTCT 
GATCCAGATG TCCTAATGTA TATGGAAACA GAGAAGCACT GCCAAGGAAA TGGAATGGGG 
GAGAAAACCA CCAAGCAAGG CAGCTCTAGG ACCTACTCGT CTATACATTC ACATCAATCC 
CAGCTTCCAC CACTAAGCCG GCAGCACTTA CCAGAGGGTG GCCATCATGA CCCAAAGTTT 
GAACCAGGGG AAAAGCAACT GAGTGGTAAA CATTGGCAGG AACATTCGGA CAGCAGGAGT 
GCTCAAGCAC GTTATGAAAG GTCTGATTTG GATCGCCACA TATGCAGGGT GAAGGCCACT 
AGCAGTGCCA GTGAAGATGA ACAAGCTGCT CCTGTGAAGC CGGCTCCTCC ACCTAAGCCC 
GAGAGATCCC ATAGTGTTCG AGAGCGGCAC CATTACAACC AGTCCAGTCT TCCTGCATAT 
TTACCAGACA ATTCAGATAG AGACCACAGT GGAACATACT CCCATCAGTC ACAGGGGCAG 
AGACAAAGCA CCGTTACTTT GCAGTCGCAT TACGACAACC TGGATGACTA TCATCCAGTA 
CCTCAATCCC AAACCACGCT AGCAAATCGG GGTTGTTCCA GTTCCTATCA TAGCCCCGGT 
TTCTCAACTC CACACAGTAA TCGTGCATAC TCCACTGCCT TGGGACAGGG TGCCTTCATA 
CAAGCTGAGC TTTCAGTGCA AAGGCCAGAG ACTGAGATAA ATGCTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32b ( XM_009295412.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293687.1 Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X3, mRNA.

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CloneID	ODa45739
Clone ID Related Accession (Same CDS sequence)	XM_009295410.3 , XM_009295412.3 , XM_009295411.2 , XM_009295412.2 , XM_009295410.2 , XM_009295411.3
Accession Version	XM_009295412.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho GTPase-activating protein 32 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295412.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGT CTCTTTTCTC CCCTCAGGGG CCTGAGGCCT 
GGACGGCACC CTGAAGAAGA CGATATCGTG CCAGAGCTTG CCAGGAGCAT CCACCCCCGA 
GAGCGACCTG ACTGGGAGGA AACCATCAGC GCAATGGCAC GAAGTGCTGA AATTCCAGAG 
CTGAGCTCTG AGCCGCTCAT GCGCTCCTGC AGCAGCACCG CCAGTATGAA GGTGAAGAAT 
GTCAAAAAGC TGTCATTCAC CAAGGGCCAT TTTCCAAAGT TAGCTGAATG TGCCCACTTC 
CACTATGAAA ACGTTGATTT TGGAACAATA CAGTTAACCC TCGCAGATGA GCAGACTGAA 
GTGACACGGA ATGGCCTGGA GTCAAAAGAG CCTGTGTATT TGGTTCAGAT CTACTGTCAG 
GGACGAAGCT GGATCGTGAG ACGAAGCTAT GAAGATTTCC GAGTCTTGGA TAAGCATCTC 
CATCTCTGCA TCTATGACCG CCGATTTTCC CAGCTCCCTG AGCTGCCCCG TTTCGACAGC 
CTGAGGGAGA GAGCGGAGGC AGTTTCCCAG ATGCTGATGG CTTACCTCTC CCGTCTCTCA 
GCCATTGCTG ATAACAAGAT CAATTGTGGG CCGGCCCTCA CGTGGATGGA GGTTGACAAC 
AAAGGGAATC ATTTGCTGGT GCATGAAGAA TCATCTATAA ATGTTCCAGC TATCGCAGCG 
GCCCACGTGA TCAAGCGCTA CATCGCACAG GCAGCTGATG AGCTGTCATT TGAGGTGGGG 
GATATTGTTT CTGTCATTGA CATGCCACCC AAAGAGGACA CCACCTGGTG GAGGGGAAAA 
CATGGTTTCC AGGTTGGCTT CTTTCCCAGC GAGTGTGTGG AGCTGATAAA CGACAAGGTC 
CCGCAGTCTG TCACCAACTC TGTGCCAAAA CCAGCGTCCC CCTGCTCTGG TCTGCGGCCG 
GCATCCTGGA GTCTGTTCCC ACCCTATTTG GAGATGGAGA GTATAAAGCA GGACAGTGCA 
TTGGCACCCG ACCCATTAAA CCCCTACAGG CTGAGCTCAG TATCCAAGAA ACACGGGAAA 
CTGATCACTT TTCTGCGCAC ATTCATGAAG TCCAGGCCGA CCAAGCAGAA GCTGAAGCAA 
AGAGGTATCC TCAGGGAGAG GGTGTTCGGC TGTGACCTAG GAGAGCATCT CCTCAACTCT 
GGCCATGATG TCCCCCAGGT ACTCAAGAGC TGCACCGAGT TCATTGAGAA GCATGGAGTG 
GTTGATGGAA TGTATCGTCT CTCTGGGATC GCCTCCAACA TTCAGAAGCT GCGGCACGAG 
TTTGATTCTG AACAGATCCC AGATCTGACA AAAGATGTTT ACATTCAGGA TATACACTGC 
GTGGGCTCCC TGTGCAAGCT CTACTTTCGA GAGCTTCCCA ACCCACTTCT CACCTACCAG 
CTGTACGAGA AATTCTCGGA GGCTGTGTCA GCAGCCACAG ACGAGGAGAG GCTGATCAAA 
ATACATGACG TCATTCAGCA GCTGCCGCCT CCGCATTATA GAACCCTTGA GTTTCTTATG 
AGGCATCTTT CACACTTGGC AACCTTCAGC TATGTAACGA ACATGCATAC TAAAAATTTG 
GCCATCGTTT GGGCCCCCAA CTTACTGAGG TCAAAGCAAA TAGAATCTGC TTGCTTCAGT 
GGGACAGCAG CCTTCATGGA AGTACGAATA CAATCGGTTG TGGTGGAGTT CATCCTGAAT 
CATGTGGATG TTCTCTTCAG TCCCAAACTG AGCTCTCTTA TTCGAGAGGG AACAGGACAC 
AACTCTTTGT CTCGGCCTAA GTCTTTGCTA GTACCGTCTC CATCCACAAA GCTGCTAACT 
CTAGAAGAGG CACAGGCACG CACACAGGCC CAGATCAACT CACCCATCAC TGCAGACAGC 
AAGTACATAG AGGTCGGAGA GGGTCCTGCA GCATTACAAG GAAAATTCCA CACAGTCATA 
GAATTCCCTA CTGAGAGGAA GAGGCAGCCC ATGAAGTCTA AGAAGTCTCC AGTTGGCAGT 
TGGCGTTCTT TTTTCAACCT GGGCAAGTCT TCTTCAATGT CGAAACGCAA ACTACAGCGT 
AATCCCAGTG AACCAAATGA ACTCAAGGCT ATGGCACTGG CTGGAGGAAG AGGAGACACT 
GGTACTCTTC GGTCAGCCAA AAGTGAAGAG TCTCTCACCT CTCTACATAA TGTTGAAGGA 
GAGTCTAAAG TGTATAGGCC CCGCAGACCT CGCTCCAGCA GTGATGCCCT CTCTGCTTCA 
TTCAATGGTG ACCTTCTGGA TACCAGACAG CACTGTAACT CGTACGACAA CCTAGGTCAT 
GGAGACAGTG ATGGGGATGA TGGTCCAATC TGTGTGCCTG CTCTCATCTC CCCACCACGC 
TCCGCTGATG ATGTGGATCT GAGCCCCCCT GACATTGGCA TGGCCTCTTT AGACTTTGAC 
CCCATGTCTT TCCAATGTAG CCTTCCTCTG GCAGAAACTT CGATTTTCTC AATTGACGCA 
GATACAAGCA GTCTAAAAAG GAGCCCTGCC AGTGGCTCAG AGTCAGTCTC GCCAGTCCGA 
GCTAAAGTTA CGAGCCGGTT TTTGTCTCCA GACCACAGTA CAGCCTTAAA GGATAAACAT 
AAACTGCTCC TTACATCTTT CTCTGAGAAA ACTCCAGTTC AGTCTGTTGA AAGCACCGAG 
ATGTCAGCAG TTGTTTCTCC TCCAAGCATC TCTGAATCAG CTGCCTCCGT GATAATGAAA 
GGACCAGAGA GCACTGTGCT TCAGCCCTCT TCCCCTCCAT CTCCACTCGA CTCTCCTACA 
AAATTGAGCT CTCTGTCAAG AACCACCGAT CTGCCCCTGA GTGAAGCCAT TCAACAAGAG 
CTTCTTTCTA AAGCTGCTAC CTTTGAGAGT AAAGACATAT CGAGCATTGA ATGTTCACAA 
CCACCACAGG TCACCTGCTC TCAAGAACAG CCAGGAGCCG TAGCTTTGGA CTCTCCAAAG 
GGCAATGTCC CTGTCAGCTC TGTGTCCCTC GTCCCTCCCC CTCCCCCACC AAAGAACGCT 
GCCCGCATGC TTGCACTAGC TCTAGCTGAA TCTGCCCAGC AGGCCACCAT TCTTTCTCAG 
AAGCAATCCT CTGGACCCCC TACACCAGTA TCCCCTCTTA GGCCTCAAGA GTTATTGGAG 
ACCCTGGACT GGCCGCTGCC TTCTTCCTTG CCATCACAGC CATCCGAGGA GGAAACTGCA 
GTAGAATCAT CAAATAAATC GGTTCCTTTG TCTTCACCAC CCTCCACTCC ACCTGAAAAA 
ATGACTCGCT CAGTCAGTCT GCACCTCACC ACAGCTGATA GTGGGAAGCC ATCCAGCATT 
TCATGCAACA GCCAGGATGT AATCTCACCA GAGACCCCAA GCCAGAGTGC TATGCCCTCT 
TGTCTGACTT CTCATGTTTC TCAAACACAA AAAAGTCCAG AAAAACAGCA GCCTGCTGTC 
GGTCAGACAC CTGTGAGCAC AGCAAAAACC ACCACCACAA CCACCACTTC AACCATCAGC 
AGTAAGGATG CTGTCATTCA ACAACCCACC TCTGAGATCC ATATGCCTGA AACAGTCCTG 
CCACCAAAAC CAAACAAGGT GCCAGATCAA CCAGTGGCAA TTCTTCAGCC TCAGCCTCAA 
ATGCAGACGC AACCTCATAC TGCATTTCGG TCTCAACCTC AAGTTCAGCC TCTTCCTCAG 
TCACAAGCAC AGCCACAGTC TCAGCCCAGA TCACAGCCCA ATGCTAGAGT TGCACCAACC 
CTGGCAGCAC CTCATGATCC TGTGGAAAAA CCATGGGAAG CCATAAAGCC TGTCCATCCA 
AAAGGAGATA GTGCTTCTCA ACAACACCAC ACTCAGGGAG TGACACCACC AACACCCCCT 
ATTCGCTCGA TTGAAAGTAA ACTAGCAGCA GCTGCACTTT GCAACACCGA GGCTGCAAAC 
CCTGCCAACT TTCATGCCAT GTTAGAGACA TCCATGAGGG GATCAGTAGA GGAGACTCCC 
CCACAGTCTC ATCCGCCTCA CCGCAGATCG TCTACTCATC AGTCAGGTTA TCTTTATCAC 
ACAAAAGGGG AGGCAGCCTT ACTGGACCCC ACCACAGAGG CATATTACCA TCAGCGGGTA 
ACCCCTTCAG GTCAGGCTAC AGTGGGGTAC CATTACCGGC CAGAGAGTGT TCCACCTCAC 
ATTTCTTACG TGTCAAAATC AGAGCCACAC ATTTCCTACA GAGGTCATGG GGATGGTCGA 
TACAATACAA TTGGACCTAG ATCCTACCAC CAGTCTATCA AATCTCGTGG TTCACTTAGA 
AGTGAGTACA TCTCTCCAAG CTCATTACAC CACTCCCATA GCTACAGCCA AGCAGATGGA 
CCAACCGTCT ACCCAACAAT TCGCAGGGTC CACTCCTTAC ATGTGCCTCC CACTGCTATT 
CGCTCAATGC CAGTCACCAG AACTGAGGTA CCACCAGATG ATGAGTTATT TTACTACCAG 
CGCCCAATCT ACCACTGCAA GTCTCACCAA CCATCATCTC AAGTCGACTA TCACGTCACA 
CAACTGCAGC CTTATTTTGA AAATGGAAGA GTTCAGTACC GCTACAGCCC CTACTCTGGC 
TCTCACGTTC TGGAATCCCC TTTCTATGAT GTGGACCATT ATGGTACTAT CCGCTTAAGG 
CACGTCCATT CCTTCAGTGG TCGGGACAGT GCCCCTCTCC TTCGATCTGG AGCAAAATCT 
GGAGGCTACC ACTACCTTTC ACGGCATGTT ATTCCTTTAA AGGAGCATGG CTTCATAAGC 
AGGGACATGC CACCTTACAG TGGTTCAAAA GGGAGTATTT ACTTTGCATG GGACCCAGAG 
GAGGCAGAAA GGCTGCGCAT ACACTCCATC CGTAGGGAAA GCAGAGCAAG GCAAAAGGTT 
AAGGGTTCTG TTATGTCTCA ATACGACAAT GTAGGACCCT ACATGCCTGT GGATGTTGAC 
ATGTTACATT TGCGCAGCAA ATCAGATCCT GGAAAAACTG TATTGGTGTC CTCGGAGAGC 
AAAGAGGCCA GATACAATGT TGTGCCTGGA TCTCAGCATG CTCCCAGACA CTTGATCTCT 
GATCCAGATG TCCTAATGTA TATGGAAACA GAGAAGCACT GCCAAGGAAA TGGAATGGGG 
GAGAAAACCA CCAAGCAAGG CAGCTCTAGG ACCTACTCGT CTATACATTC ACATCAATCC 
CAGCTTCCAC CACTAAGCCG GCAGCACTTA CCAGAGGGTG GCCATCATGA CCCAAAGTTT 
GAACCAGGGG AAAAGCAACT GAGTGGTAAA CATTGGCAGG AACATTCGGA CAGCAGGAGT 
GCTCAAGCAC GTTATGAAAG GTCTGATTTG GATCGCCACA TATGCAGGGT GAAGGCCACT 
AGCAGTGCCA GTGAAGATGA ACAAGCTGCT CCTGTGAAGC CGGCTCCTCC ACCTAAGCCC 
GAGAGATCCC ATAGTGTTCG AGAGCGGCAC CATTACAACC AGTCCAGTCT TCCTGCATAT 
TTACCAGACA ATTCAGATAG AGACCACAGT GGAACATACT CCCATCAGTC ACAGGGGCAG 
AGACAAAGCA CCGTTACTTT GCAGTCGCAT TACGACAACC TGGATGACTA TCATCCAGTA 
CCTCAATCCC AAACCACGCT AGCAAATCGG GGTTGTTCCA GTTCCTATCA TAGCCCCGGT 
TTCTCAACTC CACACAGTAA TCGTGCATAC TCCACTGCCT TGGGACAGGG TGCCTTCATA 
CAAGCTGAGC TTTCAGTGCA AAGGCCAGAG ACTGAGATAA ATGCTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293687.1
CDS	588..6458
Translation	MEAGSGAAAVVGSAALGLLGATGSHDISDSLFSPLRGLRPGRHPEEDDIVPELARSIHPRERPDWEETISAMARSAEIPELSSEPLMRSCSSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGTIQLTLADEQTEVTRNGLESKEPVYLVQIYCQGRSWIVRRSYEDFRVLDKHLHLCIYDRRFSQLPELPRFDSLRERAEAVSQMLMAYLSRLSAIADNKINCGPALTWMEVDNKGNHLLVHEESSINVPAIAAAHVIKRYIAQAADELSFEVGDIVSVIDMPPKEDTTWWRGKHGFQVGFFPSECVELINDKVPQSVTNSVPKPASPCSGLRPASWSLFPPYLEMESIKQDSALAPDPLNPYRLSSVSKKHGKLITFLRTFMKSRPTKQKLKQRGILRERVFGCDLGEHLLNSGHDVPQVLKSCTEFIEKHGVVDGMYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSEAVSAATDEERLIKIHDVIQQLPPPHYRTLEFLMRHLSHLATFSYVTNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVDVLFSPKLSSLIREGTGHNSLSRPKSLLVPSPSTKLLTLEEAQARTQAQINSPITADSKYIEVGEGPAALQGKFHTVIEFPTERKRQPMKSKKSPVGSWRSFFNLGKSSSMSKRKLQRNPSEPNELKAMALAGGRGDTGTLRSAKSEESLTSLHNVEGESKVYRPRRPRSSSDALSASFNGDLLDTRQHCNSYDNLGHGDSDGDDGPICVPALISPPRSADDVDLSPPDIGMASLDFDPMSFQCSLPLAETSIFSIDADTSSLKRSPASGSESVSPVRAKVTSRFLSPDHSTALKDKHKLLLTSFSEKTPVQSVESTEMSAVVSPPSISESAASVIMKGPESTVLQPSSPPSPLDSPTKLSSLSRTTDLPLSEAIQQELLSKAATFESKDISSIECSQPPQVTCSQEQPGAVALDSPKGNVPVSSVSLVPPPPPPKNAARMLALALAESAQQATILSQKQSSGPPTPVSPLRPQELLETLDWPLPSSLPSQPSEEETAVESSNKSVPLSSPPSTPPEKMTRSVSLHLTTADSGKPSSISCNSQDVISPETPSQSAMPSCLTSHVSQTQKSPEKQQPAVGQTPVSTAKTTTTTTTSTISSKDAVIQQPTSEIHMPETVLPPKPNKVPDQPVAILQPQPQMQTQPHTAFRSQPQVQPLPQSQAQPQSQPRSQPNARVAPTLAAPHDPVEKPWEAIKPVHPKGDSASQQHHTQGVTPPTPPIRSIESKLAAAALCNTEAANPANFHAMLETSMRGSVEETPPQSHPPHRRSSTHQSGYLYHTKGEAALLDPTTEAYYHQRVTPSGQATVGYHYRPESVPPHISYVSKSEPHISYRGHGDGRYNTIGPRSYHQSIKSRGSLRSEYISPSSLHHSHSYSQADGPTVYPTIRRVHSLHVPPTAIRSMPVTRTEVPPDDELFYYQRPIYHCKSHQPSSQVDYHVTQLQPYFENGRVQYRYSPYSGSHVLESPFYDVDHYGTIRLRHVHSFSGRDSAPLLRSGAKSGGYHYLSRHVIPLKEHGFISRDMPPYSGSKGSIYFAWDPEEAERLRIHSIRRESRARQKVKGSVMSQYDNVGPYMPVDVDMLHLRSKSDPGKTVLVSSESKEARYNVVPGSQHAPRHLISDPDVLMYMETEKHCQGNGMGEKTTKQGSSRTYSSIHSHQSQLPPLSRQHLPEGGHHDPKFEPGEKQLSGKHWQEHSDSRSAQARYERSDLDRHICRVKATSSASEDEQAAPVKPAPPPKPERSHSVRERHHYNQSSLPAYLPDNSDRDHSGTYSHQSQGQRQSTVTLQSHYDNLDDYHPVPQSQTTLANRGCSSSYHSPGFSTPHSNRAYSTALGQGAFIQAELSVQRPETEINAE

Target ORF information:

RefSeq Version	XM_009295412.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295412.3

ORF Insert Sequence:

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGT CTCTTTTCTC CCCTCAGGGG CCTGAGGCCT 
GGACGGCACC CTGAAGAAGA CGATATCGTG CCAGAGCTTG CCAGGAGCAT CCACCCCCGA 
GAGCGACCTG ACTGGGAGGA AACCATCAGC GCAATGGCAC GAAGTGCTGA AATTCCAGAG 
CTGAGCTCTG AGCCGCTCAT GCGCTCCTGC AGCAGCACCG CCAGTATGAA GGTGAAGAAT 
GTCAAAAAGC TGTCATTCAC CAAGGGCCAT TTTCCAAAGT TAGCTGAATG TGCCCACTTC 
CACTATGAAA ACGTTGATTT TGGAACAATA CAGTTAACCC TCGCAGATGA GCAGACTGAA 
GTGACACGGA ATGGCCTGGA GTCAAAAGAG CCTGTGTATT TGGTTCAGAT CTACTGTCAG 
GGACGAAGCT GGATCGTGAG ACGAAGCTAT GAAGATTTCC GAGTCTTGGA TAAGCATCTC 
CATCTCTGCA TCTATGACCG CCGATTTTCC CAGCTCCCTG AGCTGCCCCG TTTCGACAGC 
CTGAGGGAGA GAGCGGAGGC AGTTTCCCAG ATGCTGATGG CTTACCTCTC CCGTCTCTCA 
GCCATTGCTG ATAACAAGAT CAATTGTGGG CCGGCCCTCA CGTGGATGGA GGTTGACAAC 
AAAGGGAATC ATTTGCTGGT GCATGAAGAA TCATCTATAA ATGTTCCAGC TATCGCAGCG 
GCCCACGTGA TCAAGCGCTA CATCGCACAG GCAGCTGATG AGCTGTCATT TGAGGTGGGG 
GATATTGTTT CTGTCATTGA CATGCCACCC AAAGAGGACA CCACCTGGTG GAGGGGAAAA 
CATGGTTTCC AGGTTGGCTT CTTTCCCAGC GAGTGTGTGG AGCTGATAAA CGACAAGGTC 
CCGCAGTCTG TCACCAACTC TGTGCCAAAA CCAGCGTCCC CCTGCTCTGG TCTGCGGCCG 
GCATCCTGGA GTCTGTTCCC ACCCTATTTG GAGATGGAGA GTATAAAGCA GGACAGTGCA 
TTGGCACCCG ACCCATTAAA CCCCTACAGG CTGAGCTCAG TATCCAAGAA ACACGGGAAA 
CTGATCACTT TTCTGCGCAC ATTCATGAAG TCCAGGCCGA CCAAGCAGAA GCTGAAGCAA 
AGAGGTATCC TCAGGGAGAG GGTGTTCGGC TGTGACCTAG GAGAGCATCT CCTCAACTCT 
GGCCATGATG TCCCCCAGGT ACTCAAGAGC TGCACCGAGT TCATTGAGAA GCATGGAGTG 
GTTGATGGAA TGTATCGTCT CTCTGGGATC GCCTCCAACA TTCAGAAGCT GCGGCACGAG 
TTTGATTCTG AACAGATCCC AGATCTGACA AAAGATGTTT ACATTCAGGA TATACACTGC 
GTGGGCTCCC TGTGCAAGCT CTACTTTCGA GAGCTTCCCA ACCCACTTCT CACCTACCAG 
CTGTACGAGA AATTCTCGGA GGCTGTGTCA GCAGCCACAG ACGAGGAGAG GCTGATCAAA 
ATACATGACG TCATTCAGCA GCTGCCGCCT CCGCATTATA GAACCCTTGA GTTTCTTATG 
AGGCATCTTT CACACTTGGC AACCTTCAGC TATGTAACGA ACATGCATAC TAAAAATTTG 
GCCATCGTTT GGGCCCCCAA CTTACTGAGG TCAAAGCAAA TAGAATCTGC TTGCTTCAGT 
GGGACAGCAG CCTTCATGGA AGTACGAATA CAATCGGTTG TGGTGGAGTT CATCCTGAAT 
CATGTGGATG TTCTCTTCAG TCCCAAACTG AGCTCTCTTA TTCGAGAGGG AACAGGACAC 
AACTCTTTGT CTCGGCCTAA GTCTTTGCTA GTACCGTCTC CATCCACAAA GCTGCTAACT 
CTAGAAGAGG CACAGGCACG CACACAGGCC CAGATCAACT CACCCATCAC TGCAGACAGC 
AAGTACATAG AGGTCGGAGA GGGTCCTGCA GCATTACAAG GAAAATTCCA CACAGTCATA 
GAATTCCCTA CTGAGAGGAA GAGGCAGCCC ATGAAGTCTA AGAAGTCTCC AGTTGGCAGT 
TGGCGTTCTT TTTTCAACCT GGGCAAGTCT TCTTCAATGT CGAAACGCAA ACTACAGCGT 
AATCCCAGTG AACCAAATGA ACTCAAGGCT ATGGCACTGG CTGGAGGAAG AGGAGACACT 
GGTACTCTTC GGTCAGCCAA AAGTGAAGAG TCTCTCACCT CTCTACATAA TGTTGAAGGA 
GAGTCTAAAG TGTATAGGCC CCGCAGACCT CGCTCCAGCA GTGATGCCCT CTCTGCTTCA 
TTCAATGGTG ACCTTCTGGA TACCAGACAG CACTGTAACT CGTACGACAA CCTAGGTCAT 
GGAGACAGTG ATGGGGATGA TGGTCCAATC TGTGTGCCTG CTCTCATCTC CCCACCACGC 
TCCGCTGATG ATGTGGATCT GAGCCCCCCT GACATTGGCA TGGCCTCTTT AGACTTTGAC 
CCCATGTCTT TCCAATGTAG CCTTCCTCTG GCAGAAACTT CGATTTTCTC AATTGACGCA 
GATACAAGCA GTCTAAAAAG GAGCCCTGCC AGTGGCTCAG AGTCAGTCTC GCCAGTCCGA 
GCTAAAGTTA CGAGCCGGTT TTTGTCTCCA GACCACAGTA CAGCCTTAAA GGATAAACAT 
AAACTGCTCC TTACATCTTT CTCTGAGAAA ACTCCAGTTC AGTCTGTTGA AAGCACCGAG 
ATGTCAGCAG TTGTTTCTCC TCCAAGCATC TCTGAATCAG CTGCCTCCGT GATAATGAAA 
GGACCAGAGA GCACTGTGCT TCAGCCCTCT TCCCCTCCAT CTCCACTCGA CTCTCCTACA 
AAATTGAGCT CTCTGTCAAG AACCACCGAT CTGCCCCTGA GTGAAGCCAT TCAACAAGAG 
CTTCTTTCTA AAGCTGCTAC CTTTGAGAGT AAAGACATAT CGAGCATTGA ATGTTCACAA 
CCACCACAGG TCACCTGCTC TCAAGAACAG CCAGGAGCCG TAGCTTTGGA CTCTCCAAAG 
GGCAATGTCC CTGTCAGCTC TGTGTCCCTC GTCCCTCCCC CTCCCCCACC AAAGAACGCT 
GCCCGCATGC TTGCACTAGC TCTAGCTGAA TCTGCCCAGC AGGCCACCAT TCTTTCTCAG 
AAGCAATCCT CTGGACCCCC TACACCAGTA TCCCCTCTTA GGCCTCAAGA GTTATTGGAG 
ACCCTGGACT GGCCGCTGCC TTCTTCCTTG CCATCACAGC CATCCGAGGA GGAAACTGCA 
GTAGAATCAT CAAATAAATC GGTTCCTTTG TCTTCACCAC CCTCCACTCC ACCTGAAAAA 
ATGACTCGCT CAGTCAGTCT GCACCTCACC ACAGCTGATA GTGGGAAGCC ATCCAGCATT 
TCATGCAACA GCCAGGATGT AATCTCACCA GAGACCCCAA GCCAGAGTGC TATGCCCTCT 
TGTCTGACTT CTCATGTTTC TCAAACACAA AAAAGTCCAG AAAAACAGCA GCCTGCTGTC 
GGTCAGACAC CTGTGAGCAC AGCAAAAACC ACCACCACAA CCACCACTTC AACCATCAGC 
AGTAAGGATG CTGTCATTCA ACAACCCACC TCTGAGATCC ATATGCCTGA AACAGTCCTG 
CCACCAAAAC CAAACAAGGT GCCAGATCAA CCAGTGGCAA TTCTTCAGCC TCAGCCTCAA 
ATGCAGACGC AACCTCATAC TGCATTTCGG TCTCAACCTC AAGTTCAGCC TCTTCCTCAG 
TCACAAGCAC AGCCACAGTC TCAGCCCAGA TCACAGCCCA ATGCTAGAGT TGCACCAACC 
CTGGCAGCAC CTCATGATCC TGTGGAAAAA CCATGGGAAG CCATAAAGCC TGTCCATCCA 
AAAGGAGATA GTGCTTCTCA ACAACACCAC ACTCAGGGAG TGACACCACC AACACCCCCT 
ATTCGCTCGA TTGAAAGTAA ACTAGCAGCA GCTGCACTTT GCAACACCGA GGCTGCAAAC 
CCTGCCAACT TTCATGCCAT GTTAGAGACA TCCATGAGGG GATCAGTAGA GGAGACTCCC 
CCACAGTCTC ATCCGCCTCA CCGCAGATCG TCTACTCATC AGTCAGGTTA TCTTTATCAC 
ACAAAAGGGG AGGCAGCCTT ACTGGACCCC ACCACAGAGG CATATTACCA TCAGCGGGTA 
ACCCCTTCAG GTCAGGCTAC AGTGGGGTAC CATTACCGGC CAGAGAGTGT TCCACCTCAC 
ATTTCTTACG TGTCAAAATC AGAGCCACAC ATTTCCTACA GAGGTCATGG GGATGGTCGA 
TACAATACAA TTGGACCTAG ATCCTACCAC CAGTCTATCA AATCTCGTGG TTCACTTAGA 
AGTGAGTACA TCTCTCCAAG CTCATTACAC CACTCCCATA GCTACAGCCA AGCAGATGGA 
CCAACCGTCT ACCCAACAAT TCGCAGGGTC CACTCCTTAC ATGTGCCTCC CACTGCTATT 
CGCTCAATGC CAGTCACCAG AACTGAGGTA CCACCAGATG ATGAGTTATT TTACTACCAG 
CGCCCAATCT ACCACTGCAA GTCTCACCAA CCATCATCTC AAGTCGACTA TCACGTCACA 
CAACTGCAGC CTTATTTTGA AAATGGAAGA GTTCAGTACC GCTACAGCCC CTACTCTGGC 
TCTCACGTTC TGGAATCCCC TTTCTATGAT GTGGACCATT ATGGTACTAT CCGCTTAAGG 
CACGTCCATT CCTTCAGTGG TCGGGACAGT GCCCCTCTCC TTCGATCTGG AGCAAAATCT 
GGAGGCTACC ACTACCTTTC ACGGCATGTT ATTCCTTTAA AGGAGCATGG CTTCATAAGC 
AGGGACATGC CACCTTACAG TGGTTCAAAA GGGAGTATTT ACTTTGCATG GGACCCAGAG 
GAGGCAGAAA GGCTGCGCAT ACACTCCATC CGTAGGGAAA GCAGAGCAAG GCAAAAGGTT 
AAGGGTTCTG TTATGTCTCA ATACGACAAT GTAGGACCCT ACATGCCTGT GGATGTTGAC 
ATGTTACATT TGCGCAGCAA ATCAGATCCT GGAAAAACTG TATTGGTGTC CTCGGAGAGC 
AAAGAGGCCA GATACAATGT TGTGCCTGGA TCTCAGCATG CTCCCAGACA CTTGATCTCT 
GATCCAGATG TCCTAATGTA TATGGAAACA GAGAAGCACT GCCAAGGAAA TGGAATGGGG 
GAGAAAACCA CCAAGCAAGG CAGCTCTAGG ACCTACTCGT CTATACATTC ACATCAATCC 
CAGCTTCCAC CACTAAGCCG GCAGCACTTA CCAGAGGGTG GCCATCATGA CCCAAAGTTT 
GAACCAGGGG AAAAGCAACT GAGTGGTAAA CATTGGCAGG AACATTCGGA CAGCAGGAGT 
GCTCAAGCAC GTTATGAAAG GTCTGATTTG GATCGCCACA TATGCAGGGT GAAGGCCACT 
AGCAGTGCCA GTGAAGATGA ACAAGCTGCT CCTGTGAAGC CGGCTCCTCC ACCTAAGCCC 
GAGAGATCCC ATAGTGTTCG AGAGCGGCAC CATTACAACC AGTCCAGTCT TCCTGCATAT 
TTACCAGACA ATTCAGATAG AGACCACAGT GGAACATACT CCCATCAGTC ACAGGGGCAG 
AGACAAAGCA CCGTTACTTT GCAGTCGCAT TACGACAACC TGGATGACTA TCATCCAGTA 
CCTCAATCCC AAACCACGCT AGCAAATCGG GGTTGTTCCA GTTCCTATCA TAGCCCCGGT 
TTCTCAACTC CACACAGTAA TCGTGCATAC TCCACTGCCT TGGGACAGGG TGCCTTCATA 
CAAGCTGAGC TTTCAGTGCA AAGGCCAGAG ACTGAGATAA ATGCTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32b ( XM_009295411.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293686.1 Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X2, mRNA.

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CloneID	ODa45739
Clone ID Related Accession (Same CDS sequence)	XM_009295410.3 , XM_009295412.3 , XM_009295411.2 , XM_009295412.2 , XM_009295410.2 , XM_009295411.3
Accession Version	XM_009295411.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho GTPase-activating protein 32 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295411.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGT CTCTTTTCTC CCCTCAGGGG CCTGAGGCCT 
GGACGGCACC CTGAAGAAGA CGATATCGTG CCAGAGCTTG CCAGGAGCAT CCACCCCCGA 
GAGCGACCTG ACTGGGAGGA AACCATCAGC GCAATGGCAC GAAGTGCTGA AATTCCAGAG 
CTGAGCTCTG AGCCGCTCAT GCGCTCCTGC AGCAGCACCG CCAGTATGAA GGTGAAGAAT 
GTCAAAAAGC TGTCATTCAC CAAGGGCCAT TTTCCAAAGT TAGCTGAATG TGCCCACTTC 
CACTATGAAA ACGTTGATTT TGGAACAATA CAGTTAACCC TCGCAGATGA GCAGACTGAA 
GTGACACGGA ATGGCCTGGA GTCAAAAGAG CCTGTGTATT TGGTTCAGAT CTACTGTCAG 
GGACGAAGCT GGATCGTGAG ACGAAGCTAT GAAGATTTCC GAGTCTTGGA TAAGCATCTC 
CATCTCTGCA TCTATGACCG CCGATTTTCC CAGCTCCCTG AGCTGCCCCG TTTCGACAGC 
CTGAGGGAGA GAGCGGAGGC AGTTTCCCAG ATGCTGATGG CTTACCTCTC CCGTCTCTCA 
GCCATTGCTG ATAACAAGAT CAATTGTGGG CCGGCCCTCA CGTGGATGGA GGTTGACAAC 
AAAGGGAATC ATTTGCTGGT GCATGAAGAA TCATCTATAA ATGTTCCAGC TATCGCAGCG 
GCCCACGTGA TCAAGCGCTA CATCGCACAG GCAGCTGATG AGCTGTCATT TGAGGTGGGG 
GATATTGTTT CTGTCATTGA CATGCCACCC AAAGAGGACA CCACCTGGTG GAGGGGAAAA 
CATGGTTTCC AGGTTGGCTT CTTTCCCAGC GAGTGTGTGG AGCTGATAAA CGACAAGGTC 
CCGCAGTCTG TCACCAACTC TGTGCCAAAA CCAGCGTCCC CCTGCTCTGG TCTGCGGCCG 
GCATCCTGGA GTCTGTTCCC ACCCTATTTG GAGATGGAGA GTATAAAGCA GGACAGTGCA 
TTGGCACCCG ACCCATTAAA CCCCTACAGG CTGAGCTCAG TATCCAAGAA ACACGGGAAA 
CTGATCACTT TTCTGCGCAC ATTCATGAAG TCCAGGCCGA CCAAGCAGAA GCTGAAGCAA 
AGAGGTATCC TCAGGGAGAG GGTGTTCGGC TGTGACCTAG GAGAGCATCT CCTCAACTCT 
GGCCATGATG TCCCCCAGGT ACTCAAGAGC TGCACCGAGT TCATTGAGAA GCATGGAGTG 
GTTGATGGAA TGTATCGTCT CTCTGGGATC GCCTCCAACA TTCAGAAGCT GCGGCACGAG 
TTTGATTCTG AACAGATCCC AGATCTGACA AAAGATGTTT ACATTCAGGA TATACACTGC 
GTGGGCTCCC TGTGCAAGCT CTACTTTCGA GAGCTTCCCA ACCCACTTCT CACCTACCAG 
CTGTACGAGA AATTCTCGGA GGCTGTGTCA GCAGCCACAG ACGAGGAGAG GCTGATCAAA 
ATACATGACG TCATTCAGCA GCTGCCGCCT CCGCATTATA GAACCCTTGA GTTTCTTATG 
AGGCATCTTT CACACTTGGC AACCTTCAGC TATGTAACGA ACATGCATAC TAAAAATTTG 
GCCATCGTTT GGGCCCCCAA CTTACTGAGG TCAAAGCAAA TAGAATCTGC TTGCTTCAGT 
GGGACAGCAG CCTTCATGGA AGTACGAATA CAATCGGTTG TGGTGGAGTT CATCCTGAAT 
CATGTGGATG TTCTCTTCAG TCCCAAACTG AGCTCTCTTA TTCGAGAGGG AACAGGACAC 
AACTCTTTGT CTCGGCCTAA GTCTTTGCTA GTACCGTCTC CATCCACAAA GCTGCTAACT 
CTAGAAGAGG CACAGGCACG CACACAGGCC CAGATCAACT CACCCATCAC TGCAGACAGC 
AAGTACATAG AGGTCGGAGA GGGTCCTGCA GCATTACAAG GAAAATTCCA CACAGTCATA 
GAATTCCCTA CTGAGAGGAA GAGGCAGCCC ATGAAGTCTA AGAAGTCTCC AGTTGGCAGT 
TGGCGTTCTT TTTTCAACCT GGGCAAGTCT TCTTCAATGT CGAAACGCAA ACTACAGCGT 
AATCCCAGTG AACCAAATGA ACTCAAGGCT ATGGCACTGG CTGGAGGAAG AGGAGACACT 
GGTACTCTTC GGTCAGCCAA AAGTGAAGAG TCTCTCACCT CTCTACATAA TGTTGAAGGA 
GAGTCTAAAG TGTATAGGCC CCGCAGACCT CGCTCCAGCA GTGATGCCCT CTCTGCTTCA 
TTCAATGGTG ACCTTCTGGA TACCAGACAG CACTGTAACT CGTACGACAA CCTAGGTCAT 
GGAGACAGTG ATGGGGATGA TGGTCCAATC TGTGTGCCTG CTCTCATCTC CCCACCACGC 
TCCGCTGATG ATGTGGATCT GAGCCCCCCT GACATTGGCA TGGCCTCTTT AGACTTTGAC 
CCCATGTCTT TCCAATGTAG CCTTCCTCTG GCAGAAACTT CGATTTTCTC AATTGACGCA 
GATACAAGCA GTCTAAAAAG GAGCCCTGCC AGTGGCTCAG AGTCAGTCTC GCCAGTCCGA 
GCTAAAGTTA CGAGCCGGTT TTTGTCTCCA GACCACAGTA CAGCCTTAAA GGATAAACAT 
AAACTGCTCC TTACATCTTT CTCTGAGAAA ACTCCAGTTC AGTCTGTTGA AAGCACCGAG 
ATGTCAGCAG TTGTTTCTCC TCCAAGCATC TCTGAATCAG CTGCCTCCGT GATAATGAAA 
GGACCAGAGA GCACTGTGCT TCAGCCCTCT TCCCCTCCAT CTCCACTCGA CTCTCCTACA 
AAATTGAGCT CTCTGTCAAG AACCACCGAT CTGCCCCTGA GTGAAGCCAT TCAACAAGAG 
CTTCTTTCTA AAGCTGCTAC CTTTGAGAGT AAAGACATAT CGAGCATTGA ATGTTCACAA 
CCACCACAGG TCACCTGCTC TCAAGAACAG CCAGGAGCCG TAGCTTTGGA CTCTCCAAAG 
GGCAATGTCC CTGTCAGCTC TGTGTCCCTC GTCCCTCCCC CTCCCCCACC AAAGAACGCT 
GCCCGCATGC TTGCACTAGC TCTAGCTGAA TCTGCCCAGC AGGCCACCAT TCTTTCTCAG 
AAGCAATCCT CTGGACCCCC TACACCAGTA TCCCCTCTTA GGCCTCAAGA GTTATTGGAG 
ACCCTGGACT GGCCGCTGCC TTCTTCCTTG CCATCACAGC CATCCGAGGA GGAAACTGCA 
GTAGAATCAT CAAATAAATC GGTTCCTTTG TCTTCACCAC CCTCCACTCC ACCTGAAAAA 
ATGACTCGCT CAGTCAGTCT GCACCTCACC ACAGCTGATA GTGGGAAGCC ATCCAGCATT 
TCATGCAACA GCCAGGATGT AATCTCACCA GAGACCCCAA GCCAGAGTGC TATGCCCTCT 
TGTCTGACTT CTCATGTTTC TCAAACACAA AAAAGTCCAG AAAAACAGCA GCCTGCTGTC 
GGTCAGACAC CTGTGAGCAC AGCAAAAACC ACCACCACAA CCACCACTTC AACCATCAGC 
AGTAAGGATG CTGTCATTCA ACAACCCACC TCTGAGATCC ATATGCCTGA AACAGTCCTG 
CCACCAAAAC CAAACAAGGT GCCAGATCAA CCAGTGGCAA TTCTTCAGCC TCAGCCTCAA 
ATGCAGACGC AACCTCATAC TGCATTTCGG TCTCAACCTC AAGTTCAGCC TCTTCCTCAG 
TCACAAGCAC AGCCACAGTC TCAGCCCAGA TCACAGCCCA ATGCTAGAGT TGCACCAACC 
CTGGCAGCAC CTCATGATCC TGTGGAAAAA CCATGGGAAG CCATAAAGCC TGTCCATCCA 
AAAGGAGATA GTGCTTCTCA ACAACACCAC ACTCAGGGAG TGACACCACC AACACCCCCT 
ATTCGCTCGA TTGAAAGTAA ACTAGCAGCA GCTGCACTTT GCAACACCGA GGCTGCAAAC 
CCTGCCAACT TTCATGCCAT GTTAGAGACA TCCATGAGGG GATCAGTAGA GGAGACTCCC 
CCACAGTCTC ATCCGCCTCA CCGCAGATCG TCTACTCATC AGTCAGGTTA TCTTTATCAC 
ACAAAAGGGG AGGCAGCCTT ACTGGACCCC ACCACAGAGG CATATTACCA TCAGCGGGTA 
ACCCCTTCAG GTCAGGCTAC AGTGGGGTAC CATTACCGGC CAGAGAGTGT TCCACCTCAC 
ATTTCTTACG TGTCAAAATC AGAGCCACAC ATTTCCTACA GAGGTCATGG GGATGGTCGA 
TACAATACAA TTGGACCTAG ATCCTACCAC CAGTCTATCA AATCTCGTGG TTCACTTAGA 
AGTGAGTACA TCTCTCCAAG CTCATTACAC CACTCCCATA GCTACAGCCA AGCAGATGGA 
CCAACCGTCT ACCCAACAAT TCGCAGGGTC CACTCCTTAC ATGTGCCTCC CACTGCTATT 
CGCTCAATGC CAGTCACCAG AACTGAGGTA CCACCAGATG ATGAGTTATT TTACTACCAG 
CGCCCAATCT ACCACTGCAA GTCTCACCAA CCATCATCTC AAGTCGACTA TCACGTCACA 
CAACTGCAGC CTTATTTTGA AAATGGAAGA GTTCAGTACC GCTACAGCCC CTACTCTGGC 
TCTCACGTTC TGGAATCCCC TTTCTATGAT GTGGACCATT ATGGTACTAT CCGCTTAAGG 
CACGTCCATT CCTTCAGTGG TCGGGACAGT GCCCCTCTCC TTCGATCTGG AGCAAAATCT 
GGAGGCTACC ACTACCTTTC ACGGCATGTT ATTCCTTTAA AGGAGCATGG CTTCATAAGC 
AGGGACATGC CACCTTACAG TGGTTCAAAA GGGAGTATTT ACTTTGCATG GGACCCAGAG 
GAGGCAGAAA GGCTGCGCAT ACACTCCATC CGTAGGGAAA GCAGAGCAAG GCAAAAGGTT 
AAGGGTTCTG TTATGTCTCA ATACGACAAT GTAGGACCCT ACATGCCTGT GGATGTTGAC 
ATGTTACATT TGCGCAGCAA ATCAGATCCT GGAAAAACTG TATTGGTGTC CTCGGAGAGC 
AAAGAGGCCA GATACAATGT TGTGCCTGGA TCTCAGCATG CTCCCAGACA CTTGATCTCT 
GATCCAGATG TCCTAATGTA TATGGAAACA GAGAAGCACT GCCAAGGAAA TGGAATGGGG 
GAGAAAACCA CCAAGCAAGG CAGCTCTAGG ACCTACTCGT CTATACATTC ACATCAATCC 
CAGCTTCCAC CACTAAGCCG GCAGCACTTA CCAGAGGGTG GCCATCATGA CCCAAAGTTT 
GAACCAGGGG AAAAGCAACT GAGTGGTAAA CATTGGCAGG AACATTCGGA CAGCAGGAGT 
GCTCAAGCAC GTTATGAAAG GTCTGATTTG GATCGCCACA TATGCAGGGT GAAGGCCACT 
AGCAGTGCCA GTGAAGATGA ACAAGCTGCT CCTGTGAAGC CGGCTCCTCC ACCTAAGCCC 
GAGAGATCCC ATAGTGTTCG AGAGCGGCAC CATTACAACC AGTCCAGTCT TCCTGCATAT 
TTACCAGACA ATTCAGATAG AGACCACAGT GGAACATACT CCCATCAGTC ACAGGGGCAG 
AGACAAAGCA CCGTTACTTT GCAGTCGCAT TACGACAACC TGGATGACTA TCATCCAGTA 
CCTCAATCCC AAACCACGCT AGCAAATCGG GGTTGTTCCA GTTCCTATCA TAGCCCCGGT 
TTCTCAACTC CACACAGTAA TCGTGCATAC TCCACTGCCT TGGGACAGGG TGCCTTCATA 
CAAGCTGAGC TTTCAGTGCA AAGGCCAGAG ACTGAGATAA ATGCTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293686.1
CDS	710..6580
Translation	MEAGSGAAAVVGSAALGLLGATGSHDISDSLFSPLRGLRPGRHPEEDDIVPELARSIHPRERPDWEETISAMARSAEIPELSSEPLMRSCSSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGTIQLTLADEQTEVTRNGLESKEPVYLVQIYCQGRSWIVRRSYEDFRVLDKHLHLCIYDRRFSQLPELPRFDSLRERAEAVSQMLMAYLSRLSAIADNKINCGPALTWMEVDNKGNHLLVHEESSINVPAIAAAHVIKRYIAQAADELSFEVGDIVSVIDMPPKEDTTWWRGKHGFQVGFFPSECVELINDKVPQSVTNSVPKPASPCSGLRPASWSLFPPYLEMESIKQDSALAPDPLNPYRLSSVSKKHGKLITFLRTFMKSRPTKQKLKQRGILRERVFGCDLGEHLLNSGHDVPQVLKSCTEFIEKHGVVDGMYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSEAVSAATDEERLIKIHDVIQQLPPPHYRTLEFLMRHLSHLATFSYVTNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVDVLFSPKLSSLIREGTGHNSLSRPKSLLVPSPSTKLLTLEEAQARTQAQINSPITADSKYIEVGEGPAALQGKFHTVIEFPTERKRQPMKSKKSPVGSWRSFFNLGKSSSMSKRKLQRNPSEPNELKAMALAGGRGDTGTLRSAKSEESLTSLHNVEGESKVYRPRRPRSSSDALSASFNGDLLDTRQHCNSYDNLGHGDSDGDDGPICVPALISPPRSADDVDLSPPDIGMASLDFDPMSFQCSLPLAETSIFSIDADTSSLKRSPASGSESVSPVRAKVTSRFLSPDHSTALKDKHKLLLTSFSEKTPVQSVESTEMSAVVSPPSISESAASVIMKGPESTVLQPSSPPSPLDSPTKLSSLSRTTDLPLSEAIQQELLSKAATFESKDISSIECSQPPQVTCSQEQPGAVALDSPKGNVPVSSVSLVPPPPPPKNAARMLALALAESAQQATILSQKQSSGPPTPVSPLRPQELLETLDWPLPSSLPSQPSEEETAVESSNKSVPLSSPPSTPPEKMTRSVSLHLTTADSGKPSSISCNSQDVISPETPSQSAMPSCLTSHVSQTQKSPEKQQPAVGQTPVSTAKTTTTTTTSTISSKDAVIQQPTSEIHMPETVLPPKPNKVPDQPVAILQPQPQMQTQPHTAFRSQPQVQPLPQSQAQPQSQPRSQPNARVAPTLAAPHDPVEKPWEAIKPVHPKGDSASQQHHTQGVTPPTPPIRSIESKLAAAALCNTEAANPANFHAMLETSMRGSVEETPPQSHPPHRRSSTHQSGYLYHTKGEAALLDPTTEAYYHQRVTPSGQATVGYHYRPESVPPHISYVSKSEPHISYRGHGDGRYNTIGPRSYHQSIKSRGSLRSEYISPSSLHHSHSYSQADGPTVYPTIRRVHSLHVPPTAIRSMPVTRTEVPPDDELFYYQRPIYHCKSHQPSSQVDYHVTQLQPYFENGRVQYRYSPYSGSHVLESPFYDVDHYGTIRLRHVHSFSGRDSAPLLRSGAKSGGYHYLSRHVIPLKEHGFISRDMPPYSGSKGSIYFAWDPEEAERLRIHSIRRESRARQKVKGSVMSQYDNVGPYMPVDVDMLHLRSKSDPGKTVLVSSESKEARYNVVPGSQHAPRHLISDPDVLMYMETEKHCQGNGMGEKTTKQGSSRTYSSIHSHQSQLPPLSRQHLPEGGHHDPKFEPGEKQLSGKHWQEHSDSRSAQARYERSDLDRHICRVKATSSASEDEQAAPVKPAPPPKPERSHSVRERHHYNQSSLPAYLPDNSDRDHSGTYSHQSQGQRQSTVTLQSHYDNLDDYHPVPQSQTTLANRGCSSSYHSPGFSTPHSNRAYSTALGQGAFIQAELSVQRPETEINAE

Target ORF information:

RefSeq Version	XM_009295411.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295411.2

ORF Insert Sequence:

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGT CTCTTTTCTC CCCTCAGGGG CCTGAGGCCT 
GGACGGCACC CTGAAGAAGA CGATATCGTG CCAGAGCTTG CCAGGAGCAT CCACCCCCGA 
GAGCGACCTG ACTGGGAGGA AACCATCAGC GCAATGGCAC GAAGTGCTGA AATTCCAGAG 
CTGAGCTCTG AGCCGCTCAT GCGCTCCTGC AGCAGCACCG CCAGTATGAA GGTGAAGAAT 
GTCAAAAAGC TGTCATTCAC CAAGGGCCAT TTTCCAAAGT TAGCTGAATG TGCCCACTTC 
CACTATGAAA ACGTTGATTT TGGAACAATA CAGTTAACCC TCGCAGATGA GCAGACTGAA 
GTGACACGGA ATGGCCTGGA GTCAAAAGAG CCTGTGTATT TGGTTCAGAT CTACTGTCAG 
GGACGAAGCT GGATCGTGAG ACGAAGCTAT GAAGATTTCC GAGTCTTGGA TAAGCATCTC 
CATCTCTGCA TCTATGACCG CCGATTTTCC CAGCTCCCTG AGCTGCCCCG TTTCGACAGC 
CTGAGGGAGA GAGCGGAGGC AGTTTCCCAG ATGCTGATGG CTTACCTCTC CCGTCTCTCA 
GCCATTGCTG ATAACAAGAT CAATTGTGGG CCGGCCCTCA CGTGGATGGA GGTTGACAAC 
AAAGGGAATC ATTTGCTGGT GCATGAAGAA TCATCTATAA ATGTTCCAGC TATCGCAGCG 
GCCCACGTGA TCAAGCGCTA CATCGCACAG GCAGCTGATG AGCTGTCATT TGAGGTGGGG 
GATATTGTTT CTGTCATTGA CATGCCACCC AAAGAGGACA CCACCTGGTG GAGGGGAAAA 
CATGGTTTCC AGGTTGGCTT CTTTCCCAGC GAGTGTGTGG AGCTGATAAA CGACAAGGTC 
CCGCAGTCTG TCACCAACTC TGTGCCAAAA CCAGCGTCCC CCTGCTCTGG TCTGCGGCCG 
GCATCCTGGA GTCTGTTCCC ACCCTATTTG GAGATGGAGA GTATAAAGCA GGACAGTGCA 
TTGGCACCCG ACCCATTAAA CCCCTACAGG CTGAGCTCAG TATCCAAGAA ACACGGGAAA 
CTGATCACTT TTCTGCGCAC ATTCATGAAG TCCAGGCCGA CCAAGCAGAA GCTGAAGCAA 
AGAGGTATCC TCAGGGAGAG GGTGTTCGGC TGTGACCTAG GAGAGCATCT CCTCAACTCT 
GGCCATGATG TCCCCCAGGT ACTCAAGAGC TGCACCGAGT TCATTGAGAA GCATGGAGTG 
GTTGATGGAA TGTATCGTCT CTCTGGGATC GCCTCCAACA TTCAGAAGCT GCGGCACGAG 
TTTGATTCTG AACAGATCCC AGATCTGACA AAAGATGTTT ACATTCAGGA TATACACTGC 
GTGGGCTCCC TGTGCAAGCT CTACTTTCGA GAGCTTCCCA ACCCACTTCT CACCTACCAG 
CTGTACGAGA AATTCTCGGA GGCTGTGTCA GCAGCCACAG ACGAGGAGAG GCTGATCAAA 
ATACATGACG TCATTCAGCA GCTGCCGCCT CCGCATTATA GAACCCTTGA GTTTCTTATG 
AGGCATCTTT CACACTTGGC AACCTTCAGC TATGTAACGA ACATGCATAC TAAAAATTTG 
GCCATCGTTT GGGCCCCCAA CTTACTGAGG TCAAAGCAAA TAGAATCTGC TTGCTTCAGT 
GGGACAGCAG CCTTCATGGA AGTACGAATA CAATCGGTTG TGGTGGAGTT CATCCTGAAT 
CATGTGGATG TTCTCTTCAG TCCCAAACTG AGCTCTCTTA TTCGAGAGGG AACAGGACAC 
AACTCTTTGT CTCGGCCTAA GTCTTTGCTA GTACCGTCTC CATCCACAAA GCTGCTAACT 
CTAGAAGAGG CACAGGCACG CACACAGGCC CAGATCAACT CACCCATCAC TGCAGACAGC 
AAGTACATAG AGGTCGGAGA GGGTCCTGCA GCATTACAAG GAAAATTCCA CACAGTCATA 
GAATTCCCTA CTGAGAGGAA GAGGCAGCCC ATGAAGTCTA AGAAGTCTCC AGTTGGCAGT 
TGGCGTTCTT TTTTCAACCT GGGCAAGTCT TCTTCAATGT CGAAACGCAA ACTACAGCGT 
AATCCCAGTG AACCAAATGA ACTCAAGGCT ATGGCACTGG CTGGAGGAAG AGGAGACACT 
GGTACTCTTC GGTCAGCCAA AAGTGAAGAG TCTCTCACCT CTCTACATAA TGTTGAAGGA 
GAGTCTAAAG TGTATAGGCC CCGCAGACCT CGCTCCAGCA GTGATGCCCT CTCTGCTTCA 
TTCAATGGTG ACCTTCTGGA TACCAGACAG CACTGTAACT CGTACGACAA CCTAGGTCAT 
GGAGACAGTG ATGGGGATGA TGGTCCAATC TGTGTGCCTG CTCTCATCTC CCCACCACGC 
TCCGCTGATG ATGTGGATCT GAGCCCCCCT GACATTGGCA TGGCCTCTTT AGACTTTGAC 
CCCATGTCTT TCCAATGTAG CCTTCCTCTG GCAGAAACTT CGATTTTCTC AATTGACGCA 
GATACAAGCA GTCTAAAAAG GAGCCCTGCC AGTGGCTCAG AGTCAGTCTC GCCAGTCCGA 
GCTAAAGTTA CGAGCCGGTT TTTGTCTCCA GACCACAGTA CAGCCTTAAA GGATAAACAT 
AAACTGCTCC TTACATCTTT CTCTGAGAAA ACTCCAGTTC AGTCTGTTGA AAGCACCGAG 
ATGTCAGCAG TTGTTTCTCC TCCAAGCATC TCTGAATCAG CTGCCTCCGT GATAATGAAA 
GGACCAGAGA GCACTGTGCT TCAGCCCTCT TCCCCTCCAT CTCCACTCGA CTCTCCTACA 
AAATTGAGCT CTCTGTCAAG AACCACCGAT CTGCCCCTGA GTGAAGCCAT TCAACAAGAG 
CTTCTTTCTA AAGCTGCTAC CTTTGAGAGT AAAGACATAT CGAGCATTGA ATGTTCACAA 
CCACCACAGG TCACCTGCTC TCAAGAACAG CCAGGAGCCG TAGCTTTGGA CTCTCCAAAG 
GGCAATGTCC CTGTCAGCTC TGTGTCCCTC GTCCCTCCCC CTCCCCCACC AAAGAACGCT 
GCCCGCATGC TTGCACTAGC TCTAGCTGAA TCTGCCCAGC AGGCCACCAT TCTTTCTCAG 
AAGCAATCCT CTGGACCCCC TACACCAGTA TCCCCTCTTA GGCCTCAAGA GTTATTGGAG 
ACCCTGGACT GGCCGCTGCC TTCTTCCTTG CCATCACAGC CATCCGAGGA GGAAACTGCA 
GTAGAATCAT CAAATAAATC GGTTCCTTTG TCTTCACCAC CCTCCACTCC ACCTGAAAAA 
ATGACTCGCT CAGTCAGTCT GCACCTCACC ACAGCTGATA GTGGGAAGCC ATCCAGCATT 
TCATGCAACA GCCAGGATGT AATCTCACCA GAGACCCCAA GCCAGAGTGC TATGCCCTCT 
TGTCTGACTT CTCATGTTTC TCAAACACAA AAAAGTCCAG AAAAACAGCA GCCTGCTGTC 
GGTCAGACAC CTGTGAGCAC AGCAAAAACC ACCACCACAA CCACCACTTC AACCATCAGC 
AGTAAGGATG CTGTCATTCA ACAACCCACC TCTGAGATCC ATATGCCTGA AACAGTCCTG 
CCACCAAAAC CAAACAAGGT GCCAGATCAA CCAGTGGCAA TTCTTCAGCC TCAGCCTCAA 
ATGCAGACGC AACCTCATAC TGCATTTCGG TCTCAACCTC AAGTTCAGCC TCTTCCTCAG 
TCACAAGCAC AGCCACAGTC TCAGCCCAGA TCACAGCCCA ATGCTAGAGT TGCACCAACC 
CTGGCAGCAC CTCATGATCC TGTGGAAAAA CCATGGGAAG CCATAAAGCC TGTCCATCCA 
AAAGGAGATA GTGCTTCTCA ACAACACCAC ACTCAGGGAG TGACACCACC AACACCCCCT 
ATTCGCTCGA TTGAAAGTAA ACTAGCAGCA GCTGCACTTT GCAACACCGA GGCTGCAAAC 
CCTGCCAACT TTCATGCCAT GTTAGAGACA TCCATGAGGG GATCAGTAGA GGAGACTCCC 
CCACAGTCTC ATCCGCCTCA CCGCAGATCG TCTACTCATC AGTCAGGTTA TCTTTATCAC 
ACAAAAGGGG AGGCAGCCTT ACTGGACCCC ACCACAGAGG CATATTACCA TCAGCGGGTA 
ACCCCTTCAG GTCAGGCTAC AGTGGGGTAC CATTACCGGC CAGAGAGTGT TCCACCTCAC 
ATTTCTTACG TGTCAAAATC AGAGCCACAC ATTTCCTACA GAGGTCATGG GGATGGTCGA 
TACAATACAA TTGGACCTAG ATCCTACCAC CAGTCTATCA AATCTCGTGG TTCACTTAGA 
AGTGAGTACA TCTCTCCAAG CTCATTACAC CACTCCCATA GCTACAGCCA AGCAGATGGA 
CCAACCGTCT ACCCAACAAT TCGCAGGGTC CACTCCTTAC ATGTGCCTCC CACTGCTATT 
CGCTCAATGC CAGTCACCAG AACTGAGGTA CCACCAGATG ATGAGTTATT TTACTACCAG 
CGCCCAATCT ACCACTGCAA GTCTCACCAA CCATCATCTC AAGTCGACTA TCACGTCACA 
CAACTGCAGC CTTATTTTGA AAATGGAAGA GTTCAGTACC GCTACAGCCC CTACTCTGGC 
TCTCACGTTC TGGAATCCCC TTTCTATGAT GTGGACCATT ATGGTACTAT CCGCTTAAGG 
CACGTCCATT CCTTCAGTGG TCGGGACAGT GCCCCTCTCC TTCGATCTGG AGCAAAATCT 
GGAGGCTACC ACTACCTTTC ACGGCATGTT ATTCCTTTAA AGGAGCATGG CTTCATAAGC 
AGGGACATGC CACCTTACAG TGGTTCAAAA GGGAGTATTT ACTTTGCATG GGACCCAGAG 
GAGGCAGAAA GGCTGCGCAT ACACTCCATC CGTAGGGAAA GCAGAGCAAG GCAAAAGGTT 
AAGGGTTCTG TTATGTCTCA ATACGACAAT GTAGGACCCT ACATGCCTGT GGATGTTGAC 
ATGTTACATT TGCGCAGCAA ATCAGATCCT GGAAAAACTG TATTGGTGTC CTCGGAGAGC 
AAAGAGGCCA GATACAATGT TGTGCCTGGA TCTCAGCATG CTCCCAGACA CTTGATCTCT 
GATCCAGATG TCCTAATGTA TATGGAAACA GAGAAGCACT GCCAAGGAAA TGGAATGGGG 
GAGAAAACCA CCAAGCAAGG CAGCTCTAGG ACCTACTCGT CTATACATTC ACATCAATCC 
CAGCTTCCAC CACTAAGCCG GCAGCACTTA CCAGAGGGTG GCCATCATGA CCCAAAGTTT 
GAACCAGGGG AAAAGCAACT GAGTGGTAAA CATTGGCAGG AACATTCGGA CAGCAGGAGT 
GCTCAAGCAC GTTATGAAAG GTCTGATTTG GATCGCCACA TATGCAGGGT GAAGGCCACT 
AGCAGTGCCA GTGAAGATGA ACAAGCTGCT CCTGTGAAGC CGGCTCCTCC ACCTAAGCCC 
GAGAGATCCC ATAGTGTTCG AGAGCGGCAC CATTACAACC AGTCCAGTCT TCCTGCATAT 
TTACCAGACA ATTCAGATAG AGACCACAGT GGAACATACT CCCATCAGTC ACAGGGGCAG 
AGACAAAGCA CCGTTACTTT GCAGTCGCAT TACGACAACC TGGATGACTA TCATCCAGTA 
CCTCAATCCC AAACCACGCT AGCAAATCGG GGTTGTTCCA GTTCCTATCA TAGCCCCGGT 
TTCTCAACTC CACACAGTAA TCGTGCATAC TCCACTGCCT TGGGACAGGG TGCCTTCATA 
CAAGCTGAGC TTTCAGTGCA AAGGCCAGAG ACTGAGATAA ATGCTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32b ( XM_009295412.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293687.1 Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X3, mRNA.

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CloneID	ODa45739
Clone ID Related Accession (Same CDS sequence)	XM_009295410.3 , XM_009295412.3 , XM_009295411.2 , XM_009295412.2 , XM_009295410.2 , XM_009295411.3
Accession Version	XM_009295412.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho GTPase-activating protein 32 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295412.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGT CTCTTTTCTC CCCTCAGGGG CCTGAGGCCT 
GGACGGCACC CTGAAGAAGA CGATATCGTG CCAGAGCTTG CCAGGAGCAT CCACCCCCGA 
GAGCGACCTG ACTGGGAGGA AACCATCAGC GCAATGGCAC GAAGTGCTGA AATTCCAGAG 
CTGAGCTCTG AGCCGCTCAT GCGCTCCTGC AGCAGCACCG CCAGTATGAA GGTGAAGAAT 
GTCAAAAAGC TGTCATTCAC CAAGGGCCAT TTTCCAAAGT TAGCTGAATG TGCCCACTTC 
CACTATGAAA ACGTTGATTT TGGAACAATA CAGTTAACCC TCGCAGATGA GCAGACTGAA 
GTGACACGGA ATGGCCTGGA GTCAAAAGAG CCTGTGTATT TGGTTCAGAT CTACTGTCAG 
GGACGAAGCT GGATCGTGAG ACGAAGCTAT GAAGATTTCC GAGTCTTGGA TAAGCATCTC 
CATCTCTGCA TCTATGACCG CCGATTTTCC CAGCTCCCTG AGCTGCCCCG TTTCGACAGC 
CTGAGGGAGA GAGCGGAGGC AGTTTCCCAG ATGCTGATGG CTTACCTCTC CCGTCTCTCA 
GCCATTGCTG ATAACAAGAT CAATTGTGGG CCGGCCCTCA CGTGGATGGA GGTTGACAAC 
AAAGGGAATC ATTTGCTGGT GCATGAAGAA TCATCTATAA ATGTTCCAGC TATCGCAGCG 
GCCCACGTGA TCAAGCGCTA CATCGCACAG GCAGCTGATG AGCTGTCATT TGAGGTGGGG 
GATATTGTTT CTGTCATTGA CATGCCACCC AAAGAGGACA CCACCTGGTG GAGGGGAAAA 
CATGGTTTCC AGGTTGGCTT CTTTCCCAGC GAGTGTGTGG AGCTGATAAA CGACAAGGTC 
CCGCAGTCTG TCACCAACTC TGTGCCAAAA CCAGCGTCCC CCTGCTCTGG TCTGCGGCCG 
GCATCCTGGA GTCTGTTCCC ACCCTATTTG GAGATGGAGA GTATAAAGCA GGACAGTGCA 
TTGGCACCCG ACCCATTAAA CCCCTACAGG CTGAGCTCAG TATCCAAGAA ACACGGGAAA 
CTGATCACTT TTCTGCGCAC ATTCATGAAG TCCAGGCCGA CCAAGCAGAA GCTGAAGCAA 
AGAGGTATCC TCAGGGAGAG GGTGTTCGGC TGTGACCTAG GAGAGCATCT CCTCAACTCT 
GGCCATGATG TCCCCCAGGT ACTCAAGAGC TGCACCGAGT TCATTGAGAA GCATGGAGTG 
GTTGATGGAA TGTATCGTCT CTCTGGGATC GCCTCCAACA TTCAGAAGCT GCGGCACGAG 
TTTGATTCTG AACAGATCCC AGATCTGACA AAAGATGTTT ACATTCAGGA TATACACTGC 
GTGGGCTCCC TGTGCAAGCT CTACTTTCGA GAGCTTCCCA ACCCACTTCT CACCTACCAG 
CTGTACGAGA AATTCTCGGA GGCTGTGTCA GCAGCCACAG ACGAGGAGAG GCTGATCAAA 
ATACATGACG TCATTCAGCA GCTGCCGCCT CCGCATTATA GAACCCTTGA GTTTCTTATG 
AGGCATCTTT CACACTTGGC AACCTTCAGC TATGTAACGA ACATGCATAC TAAAAATTTG 
GCCATCGTTT GGGCCCCCAA CTTACTGAGG TCAAAGCAAA TAGAATCTGC TTGCTTCAGT 
GGGACAGCAG CCTTCATGGA AGTACGAATA CAATCGGTTG TGGTGGAGTT CATCCTGAAT 
CATGTGGATG TTCTCTTCAG TCCCAAACTG AGCTCTCTTA TTCGAGAGGG AACAGGACAC 
AACTCTTTGT CTCGGCCTAA GTCTTTGCTA GTACCGTCTC CATCCACAAA GCTGCTAACT 
CTAGAAGAGG CACAGGCACG CACACAGGCC CAGATCAACT CACCCATCAC TGCAGACAGC 
AAGTACATAG AGGTCGGAGA GGGTCCTGCA GCATTACAAG GAAAATTCCA CACAGTCATA 
GAATTCCCTA CTGAGAGGAA GAGGCAGCCC ATGAAGTCTA AGAAGTCTCC AGTTGGCAGT 
TGGCGTTCTT TTTTCAACCT GGGCAAGTCT TCTTCAATGT CGAAACGCAA ACTACAGCGT 
AATCCCAGTG AACCAAATGA ACTCAAGGCT ATGGCACTGG CTGGAGGAAG AGGAGACACT 
GGTACTCTTC GGTCAGCCAA AAGTGAAGAG TCTCTCACCT CTCTACATAA TGTTGAAGGA 
GAGTCTAAAG TGTATAGGCC CCGCAGACCT CGCTCCAGCA GTGATGCCCT CTCTGCTTCA 
TTCAATGGTG ACCTTCTGGA TACCAGACAG CACTGTAACT CGTACGACAA CCTAGGTCAT 
GGAGACAGTG ATGGGGATGA TGGTCCAATC TGTGTGCCTG CTCTCATCTC CCCACCACGC 
TCCGCTGATG ATGTGGATCT GAGCCCCCCT GACATTGGCA TGGCCTCTTT AGACTTTGAC 
CCCATGTCTT TCCAATGTAG CCTTCCTCTG GCAGAAACTT CGATTTTCTC AATTGACGCA 
GATACAAGCA GTCTAAAAAG GAGCCCTGCC AGTGGCTCAG AGTCAGTCTC GCCAGTCCGA 
GCTAAAGTTA CGAGCCGGTT TTTGTCTCCA GACCACAGTA CAGCCTTAAA GGATAAACAT 
AAACTGCTCC TTACATCTTT CTCTGAGAAA ACTCCAGTTC AGTCTGTTGA AAGCACCGAG 
ATGTCAGCAG TTGTTTCTCC TCCAAGCATC TCTGAATCAG CTGCCTCCGT GATAATGAAA 
GGACCAGAGA GCACTGTGCT TCAGCCCTCT TCCCCTCCAT CTCCACTCGA CTCTCCTACA 
AAATTGAGCT CTCTGTCAAG AACCACCGAT CTGCCCCTGA GTGAAGCCAT TCAACAAGAG 
CTTCTTTCTA AAGCTGCTAC CTTTGAGAGT AAAGACATAT CGAGCATTGA ATGTTCACAA 
CCACCACAGG TCACCTGCTC TCAAGAACAG CCAGGAGCCG TAGCTTTGGA CTCTCCAAAG 
GGCAATGTCC CTGTCAGCTC TGTGTCCCTC GTCCCTCCCC CTCCCCCACC AAAGAACGCT 
GCCCGCATGC TTGCACTAGC TCTAGCTGAA TCTGCCCAGC AGGCCACCAT TCTTTCTCAG 
AAGCAATCCT CTGGACCCCC TACACCAGTA TCCCCTCTTA GGCCTCAAGA GTTATTGGAG 
ACCCTGGACT GGCCGCTGCC TTCTTCCTTG CCATCACAGC CATCCGAGGA GGAAACTGCA 
GTAGAATCAT CAAATAAATC GGTTCCTTTG TCTTCACCAC CCTCCACTCC ACCTGAAAAA 
ATGACTCGCT CAGTCAGTCT GCACCTCACC ACAGCTGATA GTGGGAAGCC ATCCAGCATT 
TCATGCAACA GCCAGGATGT AATCTCACCA GAGACCCCAA GCCAGAGTGC TATGCCCTCT 
TGTCTGACTT CTCATGTTTC TCAAACACAA AAAAGTCCAG AAAAACAGCA GCCTGCTGTC 
GGTCAGACAC CTGTGAGCAC AGCAAAAACC ACCACCACAA CCACCACTTC AACCATCAGC 
AGTAAGGATG CTGTCATTCA ACAACCCACC TCTGAGATCC ATATGCCTGA AACAGTCCTG 
CCACCAAAAC CAAACAAGGT GCCAGATCAA CCAGTGGCAA TTCTTCAGCC TCAGCCTCAA 
ATGCAGACGC AACCTCATAC TGCATTTCGG TCTCAACCTC AAGTTCAGCC TCTTCCTCAG 
TCACAAGCAC AGCCACAGTC TCAGCCCAGA TCACAGCCCA ATGCTAGAGT TGCACCAACC 
CTGGCAGCAC CTCATGATCC TGTGGAAAAA CCATGGGAAG CCATAAAGCC TGTCCATCCA 
AAAGGAGATA GTGCTTCTCA ACAACACCAC ACTCAGGGAG TGACACCACC AACACCCCCT 
ATTCGCTCGA TTGAAAGTAA ACTAGCAGCA GCTGCACTTT GCAACACCGA GGCTGCAAAC 
CCTGCCAACT TTCATGCCAT GTTAGAGACA TCCATGAGGG GATCAGTAGA GGAGACTCCC 
CCACAGTCTC ATCCGCCTCA CCGCAGATCG TCTACTCATC AGTCAGGTTA TCTTTATCAC 
ACAAAAGGGG AGGCAGCCTT ACTGGACCCC ACCACAGAGG CATATTACCA TCAGCGGGTA 
ACCCCTTCAG GTCAGGCTAC AGTGGGGTAC CATTACCGGC CAGAGAGTGT TCCACCTCAC 
ATTTCTTACG TGTCAAAATC AGAGCCACAC ATTTCCTACA GAGGTCATGG GGATGGTCGA 
TACAATACAA TTGGACCTAG ATCCTACCAC CAGTCTATCA AATCTCGTGG TTCACTTAGA 
AGTGAGTACA TCTCTCCAAG CTCATTACAC CACTCCCATA GCTACAGCCA AGCAGATGGA 
CCAACCGTCT ACCCAACAAT TCGCAGGGTC CACTCCTTAC ATGTGCCTCC CACTGCTATT 
CGCTCAATGC CAGTCACCAG AACTGAGGTA CCACCAGATG ATGAGTTATT TTACTACCAG 
CGCCCAATCT ACCACTGCAA GTCTCACCAA CCATCATCTC AAGTCGACTA TCACGTCACA 
CAACTGCAGC CTTATTTTGA AAATGGAAGA GTTCAGTACC GCTACAGCCC CTACTCTGGC 
TCTCACGTTC TGGAATCCCC TTTCTATGAT GTGGACCATT ATGGTACTAT CCGCTTAAGG 
CACGTCCATT CCTTCAGTGG TCGGGACAGT GCCCCTCTCC TTCGATCTGG AGCAAAATCT 
GGAGGCTACC ACTACCTTTC ACGGCATGTT ATTCCTTTAA AGGAGCATGG CTTCATAAGC 
AGGGACATGC CACCTTACAG TGGTTCAAAA GGGAGTATTT ACTTTGCATG GGACCCAGAG 
GAGGCAGAAA GGCTGCGCAT ACACTCCATC CGTAGGGAAA GCAGAGCAAG GCAAAAGGTT 
AAGGGTTCTG TTATGTCTCA ATACGACAAT GTAGGACCCT ACATGCCTGT GGATGTTGAC 
ATGTTACATT TGCGCAGCAA ATCAGATCCT GGAAAAACTG TATTGGTGTC CTCGGAGAGC 
AAAGAGGCCA GATACAATGT TGTGCCTGGA TCTCAGCATG CTCCCAGACA CTTGATCTCT 
GATCCAGATG TCCTAATGTA TATGGAAACA GAGAAGCACT GCCAAGGAAA TGGAATGGGG 
GAGAAAACCA CCAAGCAAGG CAGCTCTAGG ACCTACTCGT CTATACATTC ACATCAATCC 
CAGCTTCCAC CACTAAGCCG GCAGCACTTA CCAGAGGGTG GCCATCATGA CCCAAAGTTT 
GAACCAGGGG AAAAGCAACT GAGTGGTAAA CATTGGCAGG AACATTCGGA CAGCAGGAGT 
GCTCAAGCAC GTTATGAAAG GTCTGATTTG GATCGCCACA TATGCAGGGT GAAGGCCACT 
AGCAGTGCCA GTGAAGATGA ACAAGCTGCT CCTGTGAAGC CGGCTCCTCC ACCTAAGCCC 
GAGAGATCCC ATAGTGTTCG AGAGCGGCAC CATTACAACC AGTCCAGTCT TCCTGCATAT 
TTACCAGACA ATTCAGATAG AGACCACAGT GGAACATACT CCCATCAGTC ACAGGGGCAG 
AGACAAAGCA CCGTTACTTT GCAGTCGCAT TACGACAACC TGGATGACTA TCATCCAGTA 
CCTCAATCCC AAACCACGCT AGCAAATCGG GGTTGTTCCA GTTCCTATCA TAGCCCCGGT 
TTCTCAACTC CACACAGTAA TCGTGCATAC TCCACTGCCT TGGGACAGGG TGCCTTCATA 
CAAGCTGAGC TTTCAGTGCA AAGGCCAGAG ACTGAGATAA ATGCTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293687.1
CDS	591..6461
Translation	MEAGSGAAAVVGSAALGLLGATGSHDISDSLFSPLRGLRPGRHPEEDDIVPELARSIHPRERPDWEETISAMARSAEIPELSSEPLMRSCSSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGTIQLTLADEQTEVTRNGLESKEPVYLVQIYCQGRSWIVRRSYEDFRVLDKHLHLCIYDRRFSQLPELPRFDSLRERAEAVSQMLMAYLSRLSAIADNKINCGPALTWMEVDNKGNHLLVHEESSINVPAIAAAHVIKRYIAQAADELSFEVGDIVSVIDMPPKEDTTWWRGKHGFQVGFFPSECVELINDKVPQSVTNSVPKPASPCSGLRPASWSLFPPYLEMESIKQDSALAPDPLNPYRLSSVSKKHGKLITFLRTFMKSRPTKQKLKQRGILRERVFGCDLGEHLLNSGHDVPQVLKSCTEFIEKHGVVDGMYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSEAVSAATDEERLIKIHDVIQQLPPPHYRTLEFLMRHLSHLATFSYVTNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVDVLFSPKLSSLIREGTGHNSLSRPKSLLVPSPSTKLLTLEEAQARTQAQINSPITADSKYIEVGEGPAALQGKFHTVIEFPTERKRQPMKSKKSPVGSWRSFFNLGKSSSMSKRKLQRNPSEPNELKAMALAGGRGDTGTLRSAKSEESLTSLHNVEGESKVYRPRRPRSSSDALSASFNGDLLDTRQHCNSYDNLGHGDSDGDDGPICVPALISPPRSADDVDLSPPDIGMASLDFDPMSFQCSLPLAETSIFSIDADTSSLKRSPASGSESVSPVRAKVTSRFLSPDHSTALKDKHKLLLTSFSEKTPVQSVESTEMSAVVSPPSISESAASVIMKGPESTVLQPSSPPSPLDSPTKLSSLSRTTDLPLSEAIQQELLSKAATFESKDISSIECSQPPQVTCSQEQPGAVALDSPKGNVPVSSVSLVPPPPPPKNAARMLALALAESAQQATILSQKQSSGPPTPVSPLRPQELLETLDWPLPSSLPSQPSEEETAVESSNKSVPLSSPPSTPPEKMTRSVSLHLTTADSGKPSSISCNSQDVISPETPSQSAMPSCLTSHVSQTQKSPEKQQPAVGQTPVSTAKTTTTTTTSTISSKDAVIQQPTSEIHMPETVLPPKPNKVPDQPVAILQPQPQMQTQPHTAFRSQPQVQPLPQSQAQPQSQPRSQPNARVAPTLAAPHDPVEKPWEAIKPVHPKGDSASQQHHTQGVTPPTPPIRSIESKLAAAALCNTEAANPANFHAMLETSMRGSVEETPPQSHPPHRRSSTHQSGYLYHTKGEAALLDPTTEAYYHQRVTPSGQATVGYHYRPESVPPHISYVSKSEPHISYRGHGDGRYNTIGPRSYHQSIKSRGSLRSEYISPSSLHHSHSYSQADGPTVYPTIRRVHSLHVPPTAIRSMPVTRTEVPPDDELFYYQRPIYHCKSHQPSSQVDYHVTQLQPYFENGRVQYRYSPYSGSHVLESPFYDVDHYGTIRLRHVHSFSGRDSAPLLRSGAKSGGYHYLSRHVIPLKEHGFISRDMPPYSGSKGSIYFAWDPEEAERLRIHSIRRESRARQKVKGSVMSQYDNVGPYMPVDVDMLHLRSKSDPGKTVLVSSESKEARYNVVPGSQHAPRHLISDPDVLMYMETEKHCQGNGMGEKTTKQGSSRTYSSIHSHQSQLPPLSRQHLPEGGHHDPKFEPGEKQLSGKHWQEHSDSRSAQARYERSDLDRHICRVKATSSASEDEQAAPVKPAPPPKPERSHSVRERHHYNQSSLPAYLPDNSDRDHSGTYSHQSQGQRQSTVTLQSHYDNLDDYHPVPQSQTTLANRGCSSSYHSPGFSTPHSNRAYSTALGQGAFIQAELSVQRPETEINAE

Target ORF information:

RefSeq Version	XM_009295412.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295412.2

ORF Insert Sequence:

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGT CTCTTTTCTC CCCTCAGGGG CCTGAGGCCT 
GGACGGCACC CTGAAGAAGA CGATATCGTG CCAGAGCTTG CCAGGAGCAT CCACCCCCGA 
GAGCGACCTG ACTGGGAGGA AACCATCAGC GCAATGGCAC GAAGTGCTGA AATTCCAGAG 
CTGAGCTCTG AGCCGCTCAT GCGCTCCTGC AGCAGCACCG CCAGTATGAA GGTGAAGAAT 
GTCAAAAAGC TGTCATTCAC CAAGGGCCAT TTTCCAAAGT TAGCTGAATG TGCCCACTTC 
CACTATGAAA ACGTTGATTT TGGAACAATA CAGTTAACCC TCGCAGATGA GCAGACTGAA 
GTGACACGGA ATGGCCTGGA GTCAAAAGAG CCTGTGTATT TGGTTCAGAT CTACTGTCAG 
GGACGAAGCT GGATCGTGAG ACGAAGCTAT GAAGATTTCC GAGTCTTGGA TAAGCATCTC 
CATCTCTGCA TCTATGACCG CCGATTTTCC CAGCTCCCTG AGCTGCCCCG TTTCGACAGC 
CTGAGGGAGA GAGCGGAGGC AGTTTCCCAG ATGCTGATGG CTTACCTCTC CCGTCTCTCA 
GCCATTGCTG ATAACAAGAT CAATTGTGGG CCGGCCCTCA CGTGGATGGA GGTTGACAAC 
AAAGGGAATC ATTTGCTGGT GCATGAAGAA TCATCTATAA ATGTTCCAGC TATCGCAGCG 
GCCCACGTGA TCAAGCGCTA CATCGCACAG GCAGCTGATG AGCTGTCATT TGAGGTGGGG 
GATATTGTTT CTGTCATTGA CATGCCACCC AAAGAGGACA CCACCTGGTG GAGGGGAAAA 
CATGGTTTCC AGGTTGGCTT CTTTCCCAGC GAGTGTGTGG AGCTGATAAA CGACAAGGTC 
CCGCAGTCTG TCACCAACTC TGTGCCAAAA CCAGCGTCCC CCTGCTCTGG TCTGCGGCCG 
GCATCCTGGA GTCTGTTCCC ACCCTATTTG GAGATGGAGA GTATAAAGCA GGACAGTGCA 
TTGGCACCCG ACCCATTAAA CCCCTACAGG CTGAGCTCAG TATCCAAGAA ACACGGGAAA 
CTGATCACTT TTCTGCGCAC ATTCATGAAG TCCAGGCCGA CCAAGCAGAA GCTGAAGCAA 
AGAGGTATCC TCAGGGAGAG GGTGTTCGGC TGTGACCTAG GAGAGCATCT CCTCAACTCT 
GGCCATGATG TCCCCCAGGT ACTCAAGAGC TGCACCGAGT TCATTGAGAA GCATGGAGTG 
GTTGATGGAA TGTATCGTCT CTCTGGGATC GCCTCCAACA TTCAGAAGCT GCGGCACGAG 
TTTGATTCTG AACAGATCCC AGATCTGACA AAAGATGTTT ACATTCAGGA TATACACTGC 
GTGGGCTCCC TGTGCAAGCT CTACTTTCGA GAGCTTCCCA ACCCACTTCT CACCTACCAG 
CTGTACGAGA AATTCTCGGA GGCTGTGTCA GCAGCCACAG ACGAGGAGAG GCTGATCAAA 
ATACATGACG TCATTCAGCA GCTGCCGCCT CCGCATTATA GAACCCTTGA GTTTCTTATG 
AGGCATCTTT CACACTTGGC AACCTTCAGC TATGTAACGA ACATGCATAC TAAAAATTTG 
GCCATCGTTT GGGCCCCCAA CTTACTGAGG TCAAAGCAAA TAGAATCTGC TTGCTTCAGT 
GGGACAGCAG CCTTCATGGA AGTACGAATA CAATCGGTTG TGGTGGAGTT CATCCTGAAT 
CATGTGGATG TTCTCTTCAG TCCCAAACTG AGCTCTCTTA TTCGAGAGGG AACAGGACAC 
AACTCTTTGT CTCGGCCTAA GTCTTTGCTA GTACCGTCTC CATCCACAAA GCTGCTAACT 
CTAGAAGAGG CACAGGCACG CACACAGGCC CAGATCAACT CACCCATCAC TGCAGACAGC 
AAGTACATAG AGGTCGGAGA GGGTCCTGCA GCATTACAAG GAAAATTCCA CACAGTCATA 
GAATTCCCTA CTGAGAGGAA GAGGCAGCCC ATGAAGTCTA AGAAGTCTCC AGTTGGCAGT 
TGGCGTTCTT TTTTCAACCT GGGCAAGTCT TCTTCAATGT CGAAACGCAA ACTACAGCGT 
AATCCCAGTG AACCAAATGA ACTCAAGGCT ATGGCACTGG CTGGAGGAAG AGGAGACACT 
GGTACTCTTC GGTCAGCCAA AAGTGAAGAG TCTCTCACCT CTCTACATAA TGTTGAAGGA 
GAGTCTAAAG TGTATAGGCC CCGCAGACCT CGCTCCAGCA GTGATGCCCT CTCTGCTTCA 
TTCAATGGTG ACCTTCTGGA TACCAGACAG CACTGTAACT CGTACGACAA CCTAGGTCAT 
GGAGACAGTG ATGGGGATGA TGGTCCAATC TGTGTGCCTG CTCTCATCTC CCCACCACGC 
TCCGCTGATG ATGTGGATCT GAGCCCCCCT GACATTGGCA TGGCCTCTTT AGACTTTGAC 
CCCATGTCTT TCCAATGTAG CCTTCCTCTG GCAGAAACTT CGATTTTCTC AATTGACGCA 
GATACAAGCA GTCTAAAAAG GAGCCCTGCC AGTGGCTCAG AGTCAGTCTC GCCAGTCCGA 
GCTAAAGTTA CGAGCCGGTT TTTGTCTCCA GACCACAGTA CAGCCTTAAA GGATAAACAT 
AAACTGCTCC TTACATCTTT CTCTGAGAAA ACTCCAGTTC AGTCTGTTGA AAGCACCGAG 
ATGTCAGCAG TTGTTTCTCC TCCAAGCATC TCTGAATCAG CTGCCTCCGT GATAATGAAA 
GGACCAGAGA GCACTGTGCT TCAGCCCTCT TCCCCTCCAT CTCCACTCGA CTCTCCTACA 
AAATTGAGCT CTCTGTCAAG AACCACCGAT CTGCCCCTGA GTGAAGCCAT TCAACAAGAG 
CTTCTTTCTA AAGCTGCTAC CTTTGAGAGT AAAGACATAT CGAGCATTGA ATGTTCACAA 
CCACCACAGG TCACCTGCTC TCAAGAACAG CCAGGAGCCG TAGCTTTGGA CTCTCCAAAG 
GGCAATGTCC CTGTCAGCTC TGTGTCCCTC GTCCCTCCCC CTCCCCCACC AAAGAACGCT 
GCCCGCATGC TTGCACTAGC TCTAGCTGAA TCTGCCCAGC AGGCCACCAT TCTTTCTCAG 
AAGCAATCCT CTGGACCCCC TACACCAGTA TCCCCTCTTA GGCCTCAAGA GTTATTGGAG 
ACCCTGGACT GGCCGCTGCC TTCTTCCTTG CCATCACAGC CATCCGAGGA GGAAACTGCA 
GTAGAATCAT CAAATAAATC GGTTCCTTTG TCTTCACCAC CCTCCACTCC ACCTGAAAAA 
ATGACTCGCT CAGTCAGTCT GCACCTCACC ACAGCTGATA GTGGGAAGCC ATCCAGCATT 
TCATGCAACA GCCAGGATGT AATCTCACCA GAGACCCCAA GCCAGAGTGC TATGCCCTCT 
TGTCTGACTT CTCATGTTTC TCAAACACAA AAAAGTCCAG AAAAACAGCA GCCTGCTGTC 
GGTCAGACAC CTGTGAGCAC AGCAAAAACC ACCACCACAA CCACCACTTC AACCATCAGC 
AGTAAGGATG CTGTCATTCA ACAACCCACC TCTGAGATCC ATATGCCTGA AACAGTCCTG 
CCACCAAAAC CAAACAAGGT GCCAGATCAA CCAGTGGCAA TTCTTCAGCC TCAGCCTCAA 
ATGCAGACGC AACCTCATAC TGCATTTCGG TCTCAACCTC AAGTTCAGCC TCTTCCTCAG 
TCACAAGCAC AGCCACAGTC TCAGCCCAGA TCACAGCCCA ATGCTAGAGT TGCACCAACC 
CTGGCAGCAC CTCATGATCC TGTGGAAAAA CCATGGGAAG CCATAAAGCC TGTCCATCCA 
AAAGGAGATA GTGCTTCTCA ACAACACCAC ACTCAGGGAG TGACACCACC AACACCCCCT 
ATTCGCTCGA TTGAAAGTAA ACTAGCAGCA GCTGCACTTT GCAACACCGA GGCTGCAAAC 
CCTGCCAACT TTCATGCCAT GTTAGAGACA TCCATGAGGG GATCAGTAGA GGAGACTCCC 
CCACAGTCTC ATCCGCCTCA CCGCAGATCG TCTACTCATC AGTCAGGTTA TCTTTATCAC 
ACAAAAGGGG AGGCAGCCTT ACTGGACCCC ACCACAGAGG CATATTACCA TCAGCGGGTA 
ACCCCTTCAG GTCAGGCTAC AGTGGGGTAC CATTACCGGC CAGAGAGTGT TCCACCTCAC 
ATTTCTTACG TGTCAAAATC AGAGCCACAC ATTTCCTACA GAGGTCATGG GGATGGTCGA 
TACAATACAA TTGGACCTAG ATCCTACCAC CAGTCTATCA AATCTCGTGG TTCACTTAGA 
AGTGAGTACA TCTCTCCAAG CTCATTACAC CACTCCCATA GCTACAGCCA AGCAGATGGA 
CCAACCGTCT ACCCAACAAT TCGCAGGGTC CACTCCTTAC ATGTGCCTCC CACTGCTATT 
CGCTCAATGC CAGTCACCAG AACTGAGGTA CCACCAGATG ATGAGTTATT TTACTACCAG 
CGCCCAATCT ACCACTGCAA GTCTCACCAA CCATCATCTC AAGTCGACTA TCACGTCACA 
CAACTGCAGC CTTATTTTGA AAATGGAAGA GTTCAGTACC GCTACAGCCC CTACTCTGGC 
TCTCACGTTC TGGAATCCCC TTTCTATGAT GTGGACCATT ATGGTACTAT CCGCTTAAGG 
CACGTCCATT CCTTCAGTGG TCGGGACAGT GCCCCTCTCC TTCGATCTGG AGCAAAATCT 
GGAGGCTACC ACTACCTTTC ACGGCATGTT ATTCCTTTAA AGGAGCATGG CTTCATAAGC 
AGGGACATGC CACCTTACAG TGGTTCAAAA GGGAGTATTT ACTTTGCATG GGACCCAGAG 
GAGGCAGAAA GGCTGCGCAT ACACTCCATC CGTAGGGAAA GCAGAGCAAG GCAAAAGGTT 
AAGGGTTCTG TTATGTCTCA ATACGACAAT GTAGGACCCT ACATGCCTGT GGATGTTGAC 
ATGTTACATT TGCGCAGCAA ATCAGATCCT GGAAAAACTG TATTGGTGTC CTCGGAGAGC 
AAAGAGGCCA GATACAATGT TGTGCCTGGA TCTCAGCATG CTCCCAGACA CTTGATCTCT 
GATCCAGATG TCCTAATGTA TATGGAAACA GAGAAGCACT GCCAAGGAAA TGGAATGGGG 
GAGAAAACCA CCAAGCAAGG CAGCTCTAGG ACCTACTCGT CTATACATTC ACATCAATCC 
CAGCTTCCAC CACTAAGCCG GCAGCACTTA CCAGAGGGTG GCCATCATGA CCCAAAGTTT 
GAACCAGGGG AAAAGCAACT GAGTGGTAAA CATTGGCAGG AACATTCGGA CAGCAGGAGT 
GCTCAAGCAC GTTATGAAAG GTCTGATTTG GATCGCCACA TATGCAGGGT GAAGGCCACT 
AGCAGTGCCA GTGAAGATGA ACAAGCTGCT CCTGTGAAGC CGGCTCCTCC ACCTAAGCCC 
GAGAGATCCC ATAGTGTTCG AGAGCGGCAC CATTACAACC AGTCCAGTCT TCCTGCATAT 
TTACCAGACA ATTCAGATAG AGACCACAGT GGAACATACT CCCATCAGTC ACAGGGGCAG 
AGACAAAGCA CCGTTACTTT GCAGTCGCAT TACGACAACC TGGATGACTA TCATCCAGTA 
CCTCAATCCC AAACCACGCT AGCAAATCGG GGTTGTTCCA GTTCCTATCA TAGCCCCGGT 
TTCTCAACTC CACACAGTAA TCGTGCATAC TCCACTGCCT TGGGACAGGG TGCCTTCATA 
CAAGCTGAGC TTTCAGTGCA AAGGCCAGAG ACTGAGATAA ATGCTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32b ( XM_009295410.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293685.1 Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X1, mRNA.

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CloneID	ODa45739
Clone ID Related Accession (Same CDS sequence)	XM_009295410.3 , XM_009295412.3 , XM_009295411.2 , XM_009295412.2 , XM_009295410.2 , XM_009295411.3
Accession Version	XM_009295410.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho GTPase-activating protein 32 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295410.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGT CTCTTTTCTC CCCTCAGGGG CCTGAGGCCT 
GGACGGCACC CTGAAGAAGA CGATATCGTG CCAGAGCTTG CCAGGAGCAT CCACCCCCGA 
GAGCGACCTG ACTGGGAGGA AACCATCAGC GCAATGGCAC GAAGTGCTGA AATTCCAGAG 
CTGAGCTCTG AGCCGCTCAT GCGCTCCTGC AGCAGCACCG CCAGTATGAA GGTGAAGAAT 
GTCAAAAAGC TGTCATTCAC CAAGGGCCAT TTTCCAAAGT TAGCTGAATG TGCCCACTTC 
CACTATGAAA ACGTTGATTT TGGAACAATA CAGTTAACCC TCGCAGATGA GCAGACTGAA 
GTGACACGGA ATGGCCTGGA GTCAAAAGAG CCTGTGTATT TGGTTCAGAT CTACTGTCAG 
GGACGAAGCT GGATCGTGAG ACGAAGCTAT GAAGATTTCC GAGTCTTGGA TAAGCATCTC 
CATCTCTGCA TCTATGACCG CCGATTTTCC CAGCTCCCTG AGCTGCCCCG TTTCGACAGC 
CTGAGGGAGA GAGCGGAGGC AGTTTCCCAG ATGCTGATGG CTTACCTCTC CCGTCTCTCA 
GCCATTGCTG ATAACAAGAT CAATTGTGGG CCGGCCCTCA CGTGGATGGA GGTTGACAAC 
AAAGGGAATC ATTTGCTGGT GCATGAAGAA TCATCTATAA ATGTTCCAGC TATCGCAGCG 
GCCCACGTGA TCAAGCGCTA CATCGCACAG GCAGCTGATG AGCTGTCATT TGAGGTGGGG 
GATATTGTTT CTGTCATTGA CATGCCACCC AAAGAGGACA CCACCTGGTG GAGGGGAAAA 
CATGGTTTCC AGGTTGGCTT CTTTCCCAGC GAGTGTGTGG AGCTGATAAA CGACAAGGTC 
CCGCAGTCTG TCACCAACTC TGTGCCAAAA CCAGCGTCCC CCTGCTCTGG TCTGCGGCCG 
GCATCCTGGA GTCTGTTCCC ACCCTATTTG GAGATGGAGA GTATAAAGCA GGACAGTGCA 
TTGGCACCCG ACCCATTAAA CCCCTACAGG CTGAGCTCAG TATCCAAGAA ACACGGGAAA 
CTGATCACTT TTCTGCGCAC ATTCATGAAG TCCAGGCCGA CCAAGCAGAA GCTGAAGCAA 
AGAGGTATCC TCAGGGAGAG GGTGTTCGGC TGTGACCTAG GAGAGCATCT CCTCAACTCT 
GGCCATGATG TCCCCCAGGT ACTCAAGAGC TGCACCGAGT TCATTGAGAA GCATGGAGTG 
GTTGATGGAA TGTATCGTCT CTCTGGGATC GCCTCCAACA TTCAGAAGCT GCGGCACGAG 
TTTGATTCTG AACAGATCCC AGATCTGACA AAAGATGTTT ACATTCAGGA TATACACTGC 
GTGGGCTCCC TGTGCAAGCT CTACTTTCGA GAGCTTCCCA ACCCACTTCT CACCTACCAG 
CTGTACGAGA AATTCTCGGA GGCTGTGTCA GCAGCCACAG ACGAGGAGAG GCTGATCAAA 
ATACATGACG TCATTCAGCA GCTGCCGCCT CCGCATTATA GAACCCTTGA GTTTCTTATG 
AGGCATCTTT CACACTTGGC AACCTTCAGC TATGTAACGA ACATGCATAC TAAAAATTTG 
GCCATCGTTT GGGCCCCCAA CTTACTGAGG TCAAAGCAAA TAGAATCTGC TTGCTTCAGT 
GGGACAGCAG CCTTCATGGA AGTACGAATA CAATCGGTTG TGGTGGAGTT CATCCTGAAT 
CATGTGGATG TTCTCTTCAG TCCCAAACTG AGCTCTCTTA TTCGAGAGGG AACAGGACAC 
AACTCTTTGT CTCGGCCTAA GTCTTTGCTA GTACCGTCTC CATCCACAAA GCTGCTAACT 
CTAGAAGAGG CACAGGCACG CACACAGGCC CAGATCAACT CACCCATCAC TGCAGACAGC 
AAGTACATAG AGGTCGGAGA GGGTCCTGCA GCATTACAAG GAAAATTCCA CACAGTCATA 
GAATTCCCTA CTGAGAGGAA GAGGCAGCCC ATGAAGTCTA AGAAGTCTCC AGTTGGCAGT 
TGGCGTTCTT TTTTCAACCT GGGCAAGTCT TCTTCAATGT CGAAACGCAA ACTACAGCGT 
AATCCCAGTG AACCAAATGA ACTCAAGGCT ATGGCACTGG CTGGAGGAAG AGGAGACACT 
GGTACTCTTC GGTCAGCCAA AAGTGAAGAG TCTCTCACCT CTCTACATAA TGTTGAAGGA 
GAGTCTAAAG TGTATAGGCC CCGCAGACCT CGCTCCAGCA GTGATGCCCT CTCTGCTTCA 
TTCAATGGTG ACCTTCTGGA TACCAGACAG CACTGTAACT CGTACGACAA CCTAGGTCAT 
GGAGACAGTG ATGGGGATGA TGGTCCAATC TGTGTGCCTG CTCTCATCTC CCCACCACGC 
TCCGCTGATG ATGTGGATCT GAGCCCCCCT GACATTGGCA TGGCCTCTTT AGACTTTGAC 
CCCATGTCTT TCCAATGTAG CCTTCCTCTG GCAGAAACTT CGATTTTCTC AATTGACGCA 
GATACAAGCA GTCTAAAAAG GAGCCCTGCC AGTGGCTCAG AGTCAGTCTC GCCAGTCCGA 
GCTAAAGTTA CGAGCCGGTT TTTGTCTCCA GACCACAGTA CAGCCTTAAA GGATAAACAT 
AAACTGCTCC TTACATCTTT CTCTGAGAAA ACTCCAGTTC AGTCTGTTGA AAGCACCGAG 
ATGTCAGCAG TTGTTTCTCC TCCAAGCATC TCTGAATCAG CTGCCTCCGT GATAATGAAA 
GGACCAGAGA GCACTGTGCT TCAGCCCTCT TCCCCTCCAT CTCCACTCGA CTCTCCTACA 
AAATTGAGCT CTCTGTCAAG AACCACCGAT CTGCCCCTGA GTGAAGCCAT TCAACAAGAG 
CTTCTTTCTA AAGCTGCTAC CTTTGAGAGT AAAGACATAT CGAGCATTGA ATGTTCACAA 
CCACCACAGG TCACCTGCTC TCAAGAACAG CCAGGAGCCG TAGCTTTGGA CTCTCCAAAG 
GGCAATGTCC CTGTCAGCTC TGTGTCCCTC GTCCCTCCCC CTCCCCCACC AAAGAACGCT 
GCCCGCATGC TTGCACTAGC TCTAGCTGAA TCTGCCCAGC AGGCCACCAT TCTTTCTCAG 
AAGCAATCCT CTGGACCCCC TACACCAGTA TCCCCTCTTA GGCCTCAAGA GTTATTGGAG 
ACCCTGGACT GGCCGCTGCC TTCTTCCTTG CCATCACAGC CATCCGAGGA GGAAACTGCA 
GTAGAATCAT CAAATAAATC GGTTCCTTTG TCTTCACCAC CCTCCACTCC ACCTGAAAAA 
ATGACTCGCT CAGTCAGTCT GCACCTCACC ACAGCTGATA GTGGGAAGCC ATCCAGCATT 
TCATGCAACA GCCAGGATGT AATCTCACCA GAGACCCCAA GCCAGAGTGC TATGCCCTCT 
TGTCTGACTT CTCATGTTTC TCAAACACAA AAAAGTCCAG AAAAACAGCA GCCTGCTGTC 
GGTCAGACAC CTGTGAGCAC AGCAAAAACC ACCACCACAA CCACCACTTC AACCATCAGC 
AGTAAGGATG CTGTCATTCA ACAACCCACC TCTGAGATCC ATATGCCTGA AACAGTCCTG 
CCACCAAAAC CAAACAAGGT GCCAGATCAA CCAGTGGCAA TTCTTCAGCC TCAGCCTCAA 
ATGCAGACGC AACCTCATAC TGCATTTCGG TCTCAACCTC AAGTTCAGCC TCTTCCTCAG 
TCACAAGCAC AGCCACAGTC TCAGCCCAGA TCACAGCCCA ATGCTAGAGT TGCACCAACC 
CTGGCAGCAC CTCATGATCC TGTGGAAAAA CCATGGGAAG CCATAAAGCC TGTCCATCCA 
AAAGGAGATA GTGCTTCTCA ACAACACCAC ACTCAGGGAG TGACACCACC AACACCCCCT 
ATTCGCTCGA TTGAAAGTAA ACTAGCAGCA GCTGCACTTT GCAACACCGA GGCTGCAAAC 
CCTGCCAACT TTCATGCCAT GTTAGAGACA TCCATGAGGG GATCAGTAGA GGAGACTCCC 
CCACAGTCTC ATCCGCCTCA CCGCAGATCG TCTACTCATC AGTCAGGTTA TCTTTATCAC 
ACAAAAGGGG AGGCAGCCTT ACTGGACCCC ACCACAGAGG CATATTACCA TCAGCGGGTA 
ACCCCTTCAG GTCAGGCTAC AGTGGGGTAC CATTACCGGC CAGAGAGTGT TCCACCTCAC 
ATTTCTTACG TGTCAAAATC AGAGCCACAC ATTTCCTACA GAGGTCATGG GGATGGTCGA 
TACAATACAA TTGGACCTAG ATCCTACCAC CAGTCTATCA AATCTCGTGG TTCACTTAGA 
AGTGAGTACA TCTCTCCAAG CTCATTACAC CACTCCCATA GCTACAGCCA AGCAGATGGA 
CCAACCGTCT ACCCAACAAT TCGCAGGGTC CACTCCTTAC ATGTGCCTCC CACTGCTATT 
CGCTCAATGC CAGTCACCAG AACTGAGGTA CCACCAGATG ATGAGTTATT TTACTACCAG 
CGCCCAATCT ACCACTGCAA GTCTCACCAA CCATCATCTC AAGTCGACTA TCACGTCACA 
CAACTGCAGC CTTATTTTGA AAATGGAAGA GTTCAGTACC GCTACAGCCC CTACTCTGGC 
TCTCACGTTC TGGAATCCCC TTTCTATGAT GTGGACCATT ATGGTACTAT CCGCTTAAGG 
CACGTCCATT CCTTCAGTGG TCGGGACAGT GCCCCTCTCC TTCGATCTGG AGCAAAATCT 
GGAGGCTACC ACTACCTTTC ACGGCATGTT ATTCCTTTAA AGGAGCATGG CTTCATAAGC 
AGGGACATGC CACCTTACAG TGGTTCAAAA GGGAGTATTT ACTTTGCATG GGACCCAGAG 
GAGGCAGAAA GGCTGCGCAT ACACTCCATC CGTAGGGAAA GCAGAGCAAG GCAAAAGGTT 
AAGGGTTCTG TTATGTCTCA ATACGACAAT GTAGGACCCT ACATGCCTGT GGATGTTGAC 
ATGTTACATT TGCGCAGCAA ATCAGATCCT GGAAAAACTG TATTGGTGTC CTCGGAGAGC 
AAAGAGGCCA GATACAATGT TGTGCCTGGA TCTCAGCATG CTCCCAGACA CTTGATCTCT 
GATCCAGATG TCCTAATGTA TATGGAAACA GAGAAGCACT GCCAAGGAAA TGGAATGGGG 
GAGAAAACCA CCAAGCAAGG CAGCTCTAGG ACCTACTCGT CTATACATTC ACATCAATCC 
CAGCTTCCAC CACTAAGCCG GCAGCACTTA CCAGAGGGTG GCCATCATGA CCCAAAGTTT 
GAACCAGGGG AAAAGCAACT GAGTGGTAAA CATTGGCAGG AACATTCGGA CAGCAGGAGT 
GCTCAAGCAC GTTATGAAAG GTCTGATTTG GATCGCCACA TATGCAGGGT GAAGGCCACT 
AGCAGTGCCA GTGAAGATGA ACAAGCTGCT CCTGTGAAGC CGGCTCCTCC ACCTAAGCCC 
GAGAGATCCC ATAGTGTTCG AGAGCGGCAC CATTACAACC AGTCCAGTCT TCCTGCATAT 
TTACCAGACA ATTCAGATAG AGACCACAGT GGAACATACT CCCATCAGTC ACAGGGGCAG 
AGACAAAGCA CCGTTACTTT GCAGTCGCAT TACGACAACC TGGATGACTA TCATCCAGTA 
CCTCAATCCC AAACCACGCT AGCAAATCGG GGTTGTTCCA GTTCCTATCA TAGCCCCGGT 
TTCTCAACTC CACACAGTAA TCGTGCATAC TCCACTGCCT TGGGACAGGG TGCCTTCATA 
CAAGCTGAGC TTTCAGTGCA AAGGCCAGAG ACTGAGATAA ATGCTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293685.1
CDS	524..6394
Translation	MEAGSGAAAVVGSAALGLLGATGSHDISDSLFSPLRGLRPGRHPEEDDIVPELARSIHPRERPDWEETISAMARSAEIPELSSEPLMRSCSSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGTIQLTLADEQTEVTRNGLESKEPVYLVQIYCQGRSWIVRRSYEDFRVLDKHLHLCIYDRRFSQLPELPRFDSLRERAEAVSQMLMAYLSRLSAIADNKINCGPALTWMEVDNKGNHLLVHEESSINVPAIAAAHVIKRYIAQAADELSFEVGDIVSVIDMPPKEDTTWWRGKHGFQVGFFPSECVELINDKVPQSVTNSVPKPASPCSGLRPASWSLFPPYLEMESIKQDSALAPDPLNPYRLSSVSKKHGKLITFLRTFMKSRPTKQKLKQRGILRERVFGCDLGEHLLNSGHDVPQVLKSCTEFIEKHGVVDGMYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSEAVSAATDEERLIKIHDVIQQLPPPHYRTLEFLMRHLSHLATFSYVTNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVDVLFSPKLSSLIREGTGHNSLSRPKSLLVPSPSTKLLTLEEAQARTQAQINSPITADSKYIEVGEGPAALQGKFHTVIEFPTERKRQPMKSKKSPVGSWRSFFNLGKSSSMSKRKLQRNPSEPNELKAMALAGGRGDTGTLRSAKSEESLTSLHNVEGESKVYRPRRPRSSSDALSASFNGDLLDTRQHCNSYDNLGHGDSDGDDGPICVPALISPPRSADDVDLSPPDIGMASLDFDPMSFQCSLPLAETSIFSIDADTSSLKRSPASGSESVSPVRAKVTSRFLSPDHSTALKDKHKLLLTSFSEKTPVQSVESTEMSAVVSPPSISESAASVIMKGPESTVLQPSSPPSPLDSPTKLSSLSRTTDLPLSEAIQQELLSKAATFESKDISSIECSQPPQVTCSQEQPGAVALDSPKGNVPVSSVSLVPPPPPPKNAARMLALALAESAQQATILSQKQSSGPPTPVSPLRPQELLETLDWPLPSSLPSQPSEEETAVESSNKSVPLSSPPSTPPEKMTRSVSLHLTTADSGKPSSISCNSQDVISPETPSQSAMPSCLTSHVSQTQKSPEKQQPAVGQTPVSTAKTTTTTTTSTISSKDAVIQQPTSEIHMPETVLPPKPNKVPDQPVAILQPQPQMQTQPHTAFRSQPQVQPLPQSQAQPQSQPRSQPNARVAPTLAAPHDPVEKPWEAIKPVHPKGDSASQQHHTQGVTPPTPPIRSIESKLAAAALCNTEAANPANFHAMLETSMRGSVEETPPQSHPPHRRSSTHQSGYLYHTKGEAALLDPTTEAYYHQRVTPSGQATVGYHYRPESVPPHISYVSKSEPHISYRGHGDGRYNTIGPRSYHQSIKSRGSLRSEYISPSSLHHSHSYSQADGPTVYPTIRRVHSLHVPPTAIRSMPVTRTEVPPDDELFYYQRPIYHCKSHQPSSQVDYHVTQLQPYFENGRVQYRYSPYSGSHVLESPFYDVDHYGTIRLRHVHSFSGRDSAPLLRSGAKSGGYHYLSRHVIPLKEHGFISRDMPPYSGSKGSIYFAWDPEEAERLRIHSIRRESRARQKVKGSVMSQYDNVGPYMPVDVDMLHLRSKSDPGKTVLVSSESKEARYNVVPGSQHAPRHLISDPDVLMYMETEKHCQGNGMGEKTTKQGSSRTYSSIHSHQSQLPPLSRQHLPEGGHHDPKFEPGEKQLSGKHWQEHSDSRSAQARYERSDLDRHICRVKATSSASEDEQAAPVKPAPPPKPERSHSVRERHHYNQSSLPAYLPDNSDRDHSGTYSHQSQGQRQSTVTLQSHYDNLDDYHPVPQSQTTLANRGCSSSYHSPGFSTPHSNRAYSTALGQGAFIQAELSVQRPETEINAE

Target ORF information:

RefSeq Version	XM_009295410.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295410.2

ORF Insert Sequence:

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGT CTCTTTTCTC CCCTCAGGGG CCTGAGGCCT 
GGACGGCACC CTGAAGAAGA CGATATCGTG CCAGAGCTTG CCAGGAGCAT CCACCCCCGA 
GAGCGACCTG ACTGGGAGGA AACCATCAGC GCAATGGCAC GAAGTGCTGA AATTCCAGAG 
CTGAGCTCTG AGCCGCTCAT GCGCTCCTGC AGCAGCACCG CCAGTATGAA GGTGAAGAAT 
GTCAAAAAGC TGTCATTCAC CAAGGGCCAT TTTCCAAAGT TAGCTGAATG TGCCCACTTC 
CACTATGAAA ACGTTGATTT TGGAACAATA CAGTTAACCC TCGCAGATGA GCAGACTGAA 
GTGACACGGA ATGGCCTGGA GTCAAAAGAG CCTGTGTATT TGGTTCAGAT CTACTGTCAG 
GGACGAAGCT GGATCGTGAG ACGAAGCTAT GAAGATTTCC GAGTCTTGGA TAAGCATCTC 
CATCTCTGCA TCTATGACCG CCGATTTTCC CAGCTCCCTG AGCTGCCCCG TTTCGACAGC 
CTGAGGGAGA GAGCGGAGGC AGTTTCCCAG ATGCTGATGG CTTACCTCTC CCGTCTCTCA 
GCCATTGCTG ATAACAAGAT CAATTGTGGG CCGGCCCTCA CGTGGATGGA GGTTGACAAC 
AAAGGGAATC ATTTGCTGGT GCATGAAGAA TCATCTATAA ATGTTCCAGC TATCGCAGCG 
GCCCACGTGA TCAAGCGCTA CATCGCACAG GCAGCTGATG AGCTGTCATT TGAGGTGGGG 
GATATTGTTT CTGTCATTGA CATGCCACCC AAAGAGGACA CCACCTGGTG GAGGGGAAAA 
CATGGTTTCC AGGTTGGCTT CTTTCCCAGC GAGTGTGTGG AGCTGATAAA CGACAAGGTC 
CCGCAGTCTG TCACCAACTC TGTGCCAAAA CCAGCGTCCC CCTGCTCTGG TCTGCGGCCG 
GCATCCTGGA GTCTGTTCCC ACCCTATTTG GAGATGGAGA GTATAAAGCA GGACAGTGCA 
TTGGCACCCG ACCCATTAAA CCCCTACAGG CTGAGCTCAG TATCCAAGAA ACACGGGAAA 
CTGATCACTT TTCTGCGCAC ATTCATGAAG TCCAGGCCGA CCAAGCAGAA GCTGAAGCAA 
AGAGGTATCC TCAGGGAGAG GGTGTTCGGC TGTGACCTAG GAGAGCATCT CCTCAACTCT 
GGCCATGATG TCCCCCAGGT ACTCAAGAGC TGCACCGAGT TCATTGAGAA GCATGGAGTG 
GTTGATGGAA TGTATCGTCT CTCTGGGATC GCCTCCAACA TTCAGAAGCT GCGGCACGAG 
TTTGATTCTG AACAGATCCC AGATCTGACA AAAGATGTTT ACATTCAGGA TATACACTGC 
GTGGGCTCCC TGTGCAAGCT CTACTTTCGA GAGCTTCCCA ACCCACTTCT CACCTACCAG 
CTGTACGAGA AATTCTCGGA GGCTGTGTCA GCAGCCACAG ACGAGGAGAG GCTGATCAAA 
ATACATGACG TCATTCAGCA GCTGCCGCCT CCGCATTATA GAACCCTTGA GTTTCTTATG 
AGGCATCTTT CACACTTGGC AACCTTCAGC TATGTAACGA ACATGCATAC TAAAAATTTG 
GCCATCGTTT GGGCCCCCAA CTTACTGAGG TCAAAGCAAA TAGAATCTGC TTGCTTCAGT 
GGGACAGCAG CCTTCATGGA AGTACGAATA CAATCGGTTG TGGTGGAGTT CATCCTGAAT 
CATGTGGATG TTCTCTTCAG TCCCAAACTG AGCTCTCTTA TTCGAGAGGG AACAGGACAC 
AACTCTTTGT CTCGGCCTAA GTCTTTGCTA GTACCGTCTC CATCCACAAA GCTGCTAACT 
CTAGAAGAGG CACAGGCACG CACACAGGCC CAGATCAACT CACCCATCAC TGCAGACAGC 
AAGTACATAG AGGTCGGAGA GGGTCCTGCA GCATTACAAG GAAAATTCCA CACAGTCATA 
GAATTCCCTA CTGAGAGGAA GAGGCAGCCC ATGAAGTCTA AGAAGTCTCC AGTTGGCAGT 
TGGCGTTCTT TTTTCAACCT GGGCAAGTCT TCTTCAATGT CGAAACGCAA ACTACAGCGT 
AATCCCAGTG AACCAAATGA ACTCAAGGCT ATGGCACTGG CTGGAGGAAG AGGAGACACT 
GGTACTCTTC GGTCAGCCAA AAGTGAAGAG TCTCTCACCT CTCTACATAA TGTTGAAGGA 
GAGTCTAAAG TGTATAGGCC CCGCAGACCT CGCTCCAGCA GTGATGCCCT CTCTGCTTCA 
TTCAATGGTG ACCTTCTGGA TACCAGACAG CACTGTAACT CGTACGACAA CCTAGGTCAT 
GGAGACAGTG ATGGGGATGA TGGTCCAATC TGTGTGCCTG CTCTCATCTC CCCACCACGC 
TCCGCTGATG ATGTGGATCT GAGCCCCCCT GACATTGGCA TGGCCTCTTT AGACTTTGAC 
CCCATGTCTT TCCAATGTAG CCTTCCTCTG GCAGAAACTT CGATTTTCTC AATTGACGCA 
GATACAAGCA GTCTAAAAAG GAGCCCTGCC AGTGGCTCAG AGTCAGTCTC GCCAGTCCGA 
GCTAAAGTTA CGAGCCGGTT TTTGTCTCCA GACCACAGTA CAGCCTTAAA GGATAAACAT 
AAACTGCTCC TTACATCTTT CTCTGAGAAA ACTCCAGTTC AGTCTGTTGA AAGCACCGAG 
ATGTCAGCAG TTGTTTCTCC TCCAAGCATC TCTGAATCAG CTGCCTCCGT GATAATGAAA 
GGACCAGAGA GCACTGTGCT TCAGCCCTCT TCCCCTCCAT CTCCACTCGA CTCTCCTACA 
AAATTGAGCT CTCTGTCAAG AACCACCGAT CTGCCCCTGA GTGAAGCCAT TCAACAAGAG 
CTTCTTTCTA AAGCTGCTAC CTTTGAGAGT AAAGACATAT CGAGCATTGA ATGTTCACAA 
CCACCACAGG TCACCTGCTC TCAAGAACAG CCAGGAGCCG TAGCTTTGGA CTCTCCAAAG 
GGCAATGTCC CTGTCAGCTC TGTGTCCCTC GTCCCTCCCC CTCCCCCACC AAAGAACGCT 
GCCCGCATGC TTGCACTAGC TCTAGCTGAA TCTGCCCAGC AGGCCACCAT TCTTTCTCAG 
AAGCAATCCT CTGGACCCCC TACACCAGTA TCCCCTCTTA GGCCTCAAGA GTTATTGGAG 
ACCCTGGACT GGCCGCTGCC TTCTTCCTTG CCATCACAGC CATCCGAGGA GGAAACTGCA 
GTAGAATCAT CAAATAAATC GGTTCCTTTG TCTTCACCAC CCTCCACTCC ACCTGAAAAA 
ATGACTCGCT CAGTCAGTCT GCACCTCACC ACAGCTGATA GTGGGAAGCC ATCCAGCATT 
TCATGCAACA GCCAGGATGT AATCTCACCA GAGACCCCAA GCCAGAGTGC TATGCCCTCT 
TGTCTGACTT CTCATGTTTC TCAAACACAA AAAAGTCCAG AAAAACAGCA GCCTGCTGTC 
GGTCAGACAC CTGTGAGCAC AGCAAAAACC ACCACCACAA CCACCACTTC AACCATCAGC 
AGTAAGGATG CTGTCATTCA ACAACCCACC TCTGAGATCC ATATGCCTGA AACAGTCCTG 
CCACCAAAAC CAAACAAGGT GCCAGATCAA CCAGTGGCAA TTCTTCAGCC TCAGCCTCAA 
ATGCAGACGC AACCTCATAC TGCATTTCGG TCTCAACCTC AAGTTCAGCC TCTTCCTCAG 
TCACAAGCAC AGCCACAGTC TCAGCCCAGA TCACAGCCCA ATGCTAGAGT TGCACCAACC 
CTGGCAGCAC CTCATGATCC TGTGGAAAAA CCATGGGAAG CCATAAAGCC TGTCCATCCA 
AAAGGAGATA GTGCTTCTCA ACAACACCAC ACTCAGGGAG TGACACCACC AACACCCCCT 
ATTCGCTCGA TTGAAAGTAA ACTAGCAGCA GCTGCACTTT GCAACACCGA GGCTGCAAAC 
CCTGCCAACT TTCATGCCAT GTTAGAGACA TCCATGAGGG GATCAGTAGA GGAGACTCCC 
CCACAGTCTC ATCCGCCTCA CCGCAGATCG TCTACTCATC AGTCAGGTTA TCTTTATCAC 
ACAAAAGGGG AGGCAGCCTT ACTGGACCCC ACCACAGAGG CATATTACCA TCAGCGGGTA 
ACCCCTTCAG GTCAGGCTAC AGTGGGGTAC CATTACCGGC CAGAGAGTGT TCCACCTCAC 
ATTTCTTACG TGTCAAAATC AGAGCCACAC ATTTCCTACA GAGGTCATGG GGATGGTCGA 
TACAATACAA TTGGACCTAG ATCCTACCAC CAGTCTATCA AATCTCGTGG TTCACTTAGA 
AGTGAGTACA TCTCTCCAAG CTCATTACAC CACTCCCATA GCTACAGCCA AGCAGATGGA 
CCAACCGTCT ACCCAACAAT TCGCAGGGTC CACTCCTTAC ATGTGCCTCC CACTGCTATT 
CGCTCAATGC CAGTCACCAG AACTGAGGTA CCACCAGATG ATGAGTTATT TTACTACCAG 
CGCCCAATCT ACCACTGCAA GTCTCACCAA CCATCATCTC AAGTCGACTA TCACGTCACA 
CAACTGCAGC CTTATTTTGA AAATGGAAGA GTTCAGTACC GCTACAGCCC CTACTCTGGC 
TCTCACGTTC TGGAATCCCC TTTCTATGAT GTGGACCATT ATGGTACTAT CCGCTTAAGG 
CACGTCCATT CCTTCAGTGG TCGGGACAGT GCCCCTCTCC TTCGATCTGG AGCAAAATCT 
GGAGGCTACC ACTACCTTTC ACGGCATGTT ATTCCTTTAA AGGAGCATGG CTTCATAAGC 
AGGGACATGC CACCTTACAG TGGTTCAAAA GGGAGTATTT ACTTTGCATG GGACCCAGAG 
GAGGCAGAAA GGCTGCGCAT ACACTCCATC CGTAGGGAAA GCAGAGCAAG GCAAAAGGTT 
AAGGGTTCTG TTATGTCTCA ATACGACAAT GTAGGACCCT ACATGCCTGT GGATGTTGAC 
ATGTTACATT TGCGCAGCAA ATCAGATCCT GGAAAAACTG TATTGGTGTC CTCGGAGAGC 
AAAGAGGCCA GATACAATGT TGTGCCTGGA TCTCAGCATG CTCCCAGACA CTTGATCTCT 
GATCCAGATG TCCTAATGTA TATGGAAACA GAGAAGCACT GCCAAGGAAA TGGAATGGGG 
GAGAAAACCA CCAAGCAAGG CAGCTCTAGG ACCTACTCGT CTATACATTC ACATCAATCC 
CAGCTTCCAC CACTAAGCCG GCAGCACTTA CCAGAGGGTG GCCATCATGA CCCAAAGTTT 
GAACCAGGGG AAAAGCAACT GAGTGGTAAA CATTGGCAGG AACATTCGGA CAGCAGGAGT 
GCTCAAGCAC GTTATGAAAG GTCTGATTTG GATCGCCACA TATGCAGGGT GAAGGCCACT 
AGCAGTGCCA GTGAAGATGA ACAAGCTGCT CCTGTGAAGC CGGCTCCTCC ACCTAAGCCC 
GAGAGATCCC ATAGTGTTCG AGAGCGGCAC CATTACAACC AGTCCAGTCT TCCTGCATAT 
TTACCAGACA ATTCAGATAG AGACCACAGT GGAACATACT CCCATCAGTC ACAGGGGCAG 
AGACAAAGCA CCGTTACTTT GCAGTCGCAT TACGACAACC TGGATGACTA TCATCCAGTA 
CCTCAATCCC AAACCACGCT AGCAAATCGG GGTTGTTCCA GTTCCTATCA TAGCCCCGGT 
TTCTCAACTC CACACAGTAA TCGTGCATAC TCCACTGCCT TGGGACAGGG TGCCTTCATA 
CAAGCTGAGC TTTCAGTGCA AAGGCCAGAG ACTGAGATAA ATGCTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32b ( XM_009295411.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293686.1 Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X2, mRNA.

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CloneID	ODa45739
Clone ID Related Accession (Same CDS sequence)	XM_009295410.3 , XM_009295412.3 , XM_009295411.2 , XM_009295412.2 , XM_009295410.2 , XM_009295411.3
Accession Version	XM_009295411.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5871bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho GTPase-activating protein 32 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295411.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGT CTCTTTTCTC CCCTCAGGGG CCTGAGGCCT 
GGACGGCACC CTGAAGAAGA CGATATCGTG CCAGAGCTTG CCAGGAGCAT CCACCCCCGA 
GAGCGACCTG ACTGGGAGGA AACCATCAGC GCAATGGCAC GAAGTGCTGA AATTCCAGAG 
CTGAGCTCTG AGCCGCTCAT GCGCTCCTGC AGCAGCACCG CCAGTATGAA GGTGAAGAAT 
GTCAAAAAGC TGTCATTCAC CAAGGGCCAT TTTCCAAAGT TAGCTGAATG TGCCCACTTC 
CACTATGAAA ACGTTGATTT TGGAACAATA CAGTTAACCC TCGCAGATGA GCAGACTGAA 
GTGACACGGA ATGGCCTGGA GTCAAAAGAG CCTGTGTATT TGGTTCAGAT CTACTGTCAG 
GGACGAAGCT GGATCGTGAG ACGAAGCTAT GAAGATTTCC GAGTCTTGGA TAAGCATCTC 
CATCTCTGCA TCTATGACCG CCGATTTTCC CAGCTCCCTG AGCTGCCCCG TTTCGACAGC 
CTGAGGGAGA GAGCGGAGGC AGTTTCCCAG ATGCTGATGG CTTACCTCTC CCGTCTCTCA 
GCCATTGCTG ATAACAAGAT CAATTGTGGG CCGGCCCTCA CGTGGATGGA GGTTGACAAC 
AAAGGGAATC ATTTGCTGGT GCATGAAGAA TCATCTATAA ATGTTCCAGC TATCGCAGCG 
GCCCACGTGA TCAAGCGCTA CATCGCACAG GCAGCTGATG AGCTGTCATT TGAGGTGGGG 
GATATTGTTT CTGTCATTGA CATGCCACCC AAAGAGGACA CCACCTGGTG GAGGGGAAAA 
CATGGTTTCC AGGTTGGCTT CTTTCCCAGC GAGTGTGTGG AGCTGATAAA CGACAAGGTC 
CCGCAGTCTG TCACCAACTC TGTGCCAAAA CCAGCGTCCC CCTGCTCTGG TCTGCGGCCG 
GCATCCTGGA GTCTGTTCCC ACCCTATTTG GAGATGGAGA GTATAAAGCA GGACAGTGCA 
TTGGCACCCG ACCCATTAAA CCCCTACAGG CTGAGCTCAG TATCCAAGAA ACACGGGAAA 
CTGATCACTT TTCTGCGCAC ATTCATGAAG TCCAGGCCGA CCAAGCAGAA GCTGAAGCAA 
AGAGGTATCC TCAGGGAGAG GGTGTTCGGC TGTGACCTAG GAGAGCATCT CCTCAACTCT 
GGCCATGATG TCCCCCAGGT ACTCAAGAGC TGCACCGAGT TCATTGAGAA GCATGGAGTG 
GTTGATGGAA TGTATCGTCT CTCTGGGATC GCCTCCAACA TTCAGAAGCT GCGGCACGAG 
TTTGATTCTG AACAGATCCC AGATCTGACA AAAGATGTTT ACATTCAGGA TATACACTGC 
GTGGGCTCCC TGTGCAAGCT CTACTTTCGA GAGCTTCCCA ACCCACTTCT CACCTACCAG 
CTGTACGAGA AATTCTCGGA GGCTGTGTCA GCAGCCACAG ACGAGGAGAG GCTGATCAAA 
ATACATGACG TCATTCAGCA GCTGCCGCCT CCGCATTATA GAACCCTTGA GTTTCTTATG 
AGGCATCTTT CACACTTGGC AACCTTCAGC TATGTAACGA ACATGCATAC TAAAAATTTG 
GCCATCGTTT GGGCCCCCAA CTTACTGAGG TCAAAGCAAA TAGAATCTGC TTGCTTCAGT 
GGGACAGCAG CCTTCATGGA AGTACGAATA CAATCGGTTG TGGTGGAGTT CATCCTGAAT 
CATGTGGATG TTCTCTTCAG TCCCAAACTG AGCTCTCTTA TTCGAGAGGG AACAGGACAC 
AACTCTTTGT CTCGGCCTAA GTCTTTGCTA GTACCGTCTC CATCCACAAA GCTGCTAACT 
CTAGAAGAGG CACAGGCACG CACACAGGCC CAGATCAACT CACCCATCAC TGCAGACAGC 
AAGTACATAG AGGTCGGAGA GGGTCCTGCA GCATTACAAG GAAAATTCCA CACAGTCATA 
GAATTCCCTA CTGAGAGGAA GAGGCAGCCC ATGAAGTCTA AGAAGTCTCC AGTTGGCAGT 
TGGCGTTCTT TTTTCAACCT GGGCAAGTCT TCTTCAATGT CGAAACGCAA ACTACAGCGT 
AATCCCAGTG AACCAAATGA ACTCAAGGCT ATGGCACTGG CTGGAGGAAG AGGAGACACT 
GGTACTCTTC GGTCAGCCAA AAGTGAAGAG TCTCTCACCT CTCTACATAA TGTTGAAGGA 
GAGTCTAAAG TGTATAGGCC CCGCAGACCT CGCTCCAGCA GTGATGCCCT CTCTGCTTCA 
TTCAATGGTG ACCTTCTGGA TACCAGACAG CACTGTAACT CGTACGACAA CCTAGGTCAT 
GGAGACAGTG ATGGGGATGA TGGTCCAATC TGTGTGCCTG CTCTCATCTC CCCACCACGC 
TCCGCTGATG ATGTGGATCT GAGCCCCCCT GACATTGGCA TGGCCTCTTT AGACTTTGAC 
CCCATGTCTT TCCAATGTAG CCTTCCTCTG GCAGAAACTT CGATTTTCTC AATTGACGCA 
GATACAAGCA GTCTAAAAAG GAGCCCTGCC AGTGGCTCAG AGTCAGTCTC GCCAGTCCGA 
GCTAAAGTTA CGAGCCGGTT TTTGTCTCCA GACCACAGTA CAGCCTTAAA GGATAAACAT 
AAACTGCTCC TTACATCTTT CTCTGAGAAA ACTCCAGTTC AGTCTGTTGA AAGCACCGAG 
ATGTCAGCAG TTGTTTCTCC TCCAAGCATC TCTGAATCAG CTGCCTCCGT GATAATGAAA 
GGACCAGAGA GCACTGTGCT TCAGCCCTCT TCCCCTCCAT CTCCACTCGA CTCTCCTACA 
AAATTGAGCT CTCTGTCAAG AACCACCGAT CTGCCCCTGA GTGAAGCCAT TCAACAAGAG 
CTTCTTTCTA AAGCTGCTAC CTTTGAGAGT AAAGACATAT CGAGCATTGA ATGTTCACAA 
CCACCACAGG TCACCTGCTC TCAAGAACAG CCAGGAGCCG TAGCTTTGGA CTCTCCAAAG 
GGCAATGTCC CTGTCAGCTC TGTGTCCCTC GTCCCTCCCC CTCCCCCACC AAAGAACGCT 
GCCCGCATGC TTGCACTAGC TCTAGCTGAA TCTGCCCAGC AGGCCACCAT TCTTTCTCAG 
AAGCAATCCT CTGGACCCCC TACACCAGTA TCCCCTCTTA GGCCTCAAGA GTTATTGGAG 
ACCCTGGACT GGCCGCTGCC TTCTTCCTTG CCATCACAGC CATCCGAGGA GGAAACTGCA 
GTAGAATCAT CAAATAAATC GGTTCCTTTG TCTTCACCAC CCTCCACTCC ACCTGAAAAA 
ATGACTCGCT CAGTCAGTCT GCACCTCACC ACAGCTGATA GTGGGAAGCC ATCCAGCATT 
TCATGCAACA GCCAGGATGT AATCTCACCA GAGACCCCAA GCCAGAGTGC TATGCCCTCT 
TGTCTGACTT CTCATGTTTC TCAAACACAA AAAAGTCCAG AAAAACAGCA GCCTGCTGTC 
GGTCAGACAC CTGTGAGCAC AGCAAAAACC ACCACCACAA CCACCACTTC AACCATCAGC 
AGTAAGGATG CTGTCATTCA ACAACCCACC TCTGAGATCC ATATGCCTGA AACAGTCCTG 
CCACCAAAAC CAAACAAGGT GCCAGATCAA CCAGTGGCAA TTCTTCAGCC TCAGCCTCAA 
ATGCAGACGC AACCTCATAC TGCATTTCGG TCTCAACCTC AAGTTCAGCC TCTTCCTCAG 
TCACAAGCAC AGCCACAGTC TCAGCCCAGA TCACAGCCCA ATGCTAGAGT TGCACCAACC 
CTGGCAGCAC CTCATGATCC TGTGGAAAAA CCATGGGAAG CCATAAAGCC TGTCCATCCA 
AAAGGAGATA GTGCTTCTCA ACAACACCAC ACTCAGGGAG TGACACCACC AACACCCCCT 
ATTCGCTCGA TTGAAAGTAA ACTAGCAGCA GCTGCACTTT GCAACACCGA GGCTGCAAAC 
CCTGCCAACT TTCATGCCAT GTTAGAGACA TCCATGAGGG GATCAGTAGA GGAGACTCCC 
CCACAGTCTC ATCCGCCTCA CCGCAGATCG TCTACTCATC AGTCAGGTTA TCTTTATCAC 
ACAAAAGGGG AGGCAGCCTT ACTGGACCCC ACCACAGAGG CATATTACCA TCAGCGGGTA 
ACCCCTTCAG GTCAGGCTAC AGTGGGGTAC CATTACCGGC CAGAGAGTGT TCCACCTCAC 
ATTTCTTACG TGTCAAAATC AGAGCCACAC ATTTCCTACA GAGGTCATGG GGATGGTCGA 
TACAATACAA TTGGACCTAG ATCCTACCAC CAGTCTATCA AATCTCGTGG TTCACTTAGA 
AGTGAGTACA TCTCTCCAAG CTCATTACAC CACTCCCATA GCTACAGCCA AGCAGATGGA 
CCAACCGTCT ACCCAACAAT TCGCAGGGTC CACTCCTTAC ATGTGCCTCC CACTGCTATT 
CGCTCAATGC CAGTCACCAG AACTGAGGTA CCACCAGATG ATGAGTTATT TTACTACCAG 
CGCCCAATCT ACCACTGCAA GTCTCACCAA CCATCATCTC AAGTCGACTA TCACGTCACA 
CAACTGCAGC CTTATTTTGA AAATGGAAGA GTTCAGTACC GCTACAGCCC CTACTCTGGC 
TCTCACGTTC TGGAATCCCC TTTCTATGAT GTGGACCATT ATGGTACTAT CCGCTTAAGG 
CACGTCCATT CCTTCAGTGG TCGGGACAGT GCCCCTCTCC TTCGATCTGG AGCAAAATCT 
GGAGGCTACC ACTACCTTTC ACGGCATGTT ATTCCTTTAA AGGAGCATGG CTTCATAAGC 
AGGGACATGC CACCTTACAG TGGTTCAAAA GGGAGTATTT ACTTTGCATG GGACCCAGAG 
GAGGCAGAAA GGCTGCGCAT ACACTCCATC CGTAGGGAAA GCAGAGCAAG GCAAAAGGTT 
AAGGGTTCTG TTATGTCTCA ATACGACAAT GTAGGACCCT ACATGCCTGT GGATGTTGAC 
ATGTTACATT TGCGCAGCAA ATCAGATCCT GGAAAAACTG TATTGGTGTC CTCGGAGAGC 
AAAGAGGCCA GATACAATGT TGTGCCTGGA TCTCAGCATG CTCCCAGACA CTTGATCTCT 
GATCCAGATG TCCTAATGTA TATGGAAACA GAGAAGCACT GCCAAGGAAA TGGAATGGGG 
GAGAAAACCA CCAAGCAAGG CAGCTCTAGG ACCTACTCGT CTATACATTC ACATCAATCC 
CAGCTTCCAC CACTAAGCCG GCAGCACTTA CCAGAGGGTG GCCATCATGA CCCAAAGTTT 
GAACCAGGGG AAAAGCAACT GAGTGGTAAA CATTGGCAGG AACATTCGGA CAGCAGGAGT 
GCTCAAGCAC GTTATGAAAG GTCTGATTTG GATCGCCACA TATGCAGGGT GAAGGCCACT 
AGCAGTGCCA GTGAAGATGA ACAAGCTGCT CCTGTGAAGC CGGCTCCTCC ACCTAAGCCC 
GAGAGATCCC ATAGTGTTCG AGAGCGGCAC CATTACAACC AGTCCAGTCT TCCTGCATAT 
TTACCAGACA ATTCAGATAG AGACCACAGT GGAACATACT CCCATCAGTC ACAGGGGCAG 
AGACAAAGCA CCGTTACTTT GCAGTCGCAT TACGACAACC TGGATGACTA TCATCCAGTA 
CCTCAATCCC AAACCACGCT AGCAAATCGG GGTTGTTCCA GTTCCTATCA TAGCCCCGGT 
TTCTCAACTC CACACAGTAA TCGTGCATAC TCCACTGCCT TGGGACAGGG TGCCTTCATA 
CAAGCTGAGC TTTCAGTGCA AAGGCCAGAG ACTGAGATAA ATGCTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293686.1
CDS	707..6577
Translation	MEAGSGAAAVVGSAALGLLGATGSHDISDSLFSPLRGLRPGRHPEEDDIVPELARSIHPRERPDWEETISAMARSAEIPELSSEPLMRSCSSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGTIQLTLADEQTEVTRNGLESKEPVYLVQIYCQGRSWIVRRSYEDFRVLDKHLHLCIYDRRFSQLPELPRFDSLRERAEAVSQMLMAYLSRLSAIADNKINCGPALTWMEVDNKGNHLLVHEESSINVPAIAAAHVIKRYIAQAADELSFEVGDIVSVIDMPPKEDTTWWRGKHGFQVGFFPSECVELINDKVPQSVTNSVPKPASPCSGLRPASWSLFPPYLEMESIKQDSALAPDPLNPYRLSSVSKKHGKLITFLRTFMKSRPTKQKLKQRGILRERVFGCDLGEHLLNSGHDVPQVLKSCTEFIEKHGVVDGMYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSEAVSAATDEERLIKIHDVIQQLPPPHYRTLEFLMRHLSHLATFSYVTNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVDVLFSPKLSSLIREGTGHNSLSRPKSLLVPSPSTKLLTLEEAQARTQAQINSPITADSKYIEVGEGPAALQGKFHTVIEFPTERKRQPMKSKKSPVGSWRSFFNLGKSSSMSKRKLQRNPSEPNELKAMALAGGRGDTGTLRSAKSEESLTSLHNVEGESKVYRPRRPRSSSDALSASFNGDLLDTRQHCNSYDNLGHGDSDGDDGPICVPALISPPRSADDVDLSPPDIGMASLDFDPMSFQCSLPLAETSIFSIDADTSSLKRSPASGSESVSPVRAKVTSRFLSPDHSTALKDKHKLLLTSFSEKTPVQSVESTEMSAVVSPPSISESAASVIMKGPESTVLQPSSPPSPLDSPTKLSSLSRTTDLPLSEAIQQELLSKAATFESKDISSIECSQPPQVTCSQEQPGAVALDSPKGNVPVSSVSLVPPPPPPKNAARMLALALAESAQQATILSQKQSSGPPTPVSPLRPQELLETLDWPLPSSLPSQPSEEETAVESSNKSVPLSSPPSTPPEKMTRSVSLHLTTADSGKPSSISCNSQDVISPETPSQSAMPSCLTSHVSQTQKSPEKQQPAVGQTPVSTAKTTTTTTTSTISSKDAVIQQPTSEIHMPETVLPPKPNKVPDQPVAILQPQPQMQTQPHTAFRSQPQVQPLPQSQAQPQSQPRSQPNARVAPTLAAPHDPVEKPWEAIKPVHPKGDSASQQHHTQGVTPPTPPIRSIESKLAAAALCNTEAANPANFHAMLETSMRGSVEETPPQSHPPHRRSSTHQSGYLYHTKGEAALLDPTTEAYYHQRVTPSGQATVGYHYRPESVPPHISYVSKSEPHISYRGHGDGRYNTIGPRSYHQSIKSRGSLRSEYISPSSLHHSHSYSQADGPTVYPTIRRVHSLHVPPTAIRSMPVTRTEVPPDDELFYYQRPIYHCKSHQPSSQVDYHVTQLQPYFENGRVQYRYSPYSGSHVLESPFYDVDHYGTIRLRHVHSFSGRDSAPLLRSGAKSGGYHYLSRHVIPLKEHGFISRDMPPYSGSKGSIYFAWDPEEAERLRIHSIRRESRARQKVKGSVMSQYDNVGPYMPVDVDMLHLRSKSDPGKTVLVSSESKEARYNVVPGSQHAPRHLISDPDVLMYMETEKHCQGNGMGEKTTKQGSSRTYSSIHSHQSQLPPLSRQHLPEGGHHDPKFEPGEKQLSGKHWQEHSDSRSAQARYERSDLDRHICRVKATSSASEDEQAAPVKPAPPPKPERSHSVRERHHYNQSSLPAYLPDNSDRDHSGTYSHQSQGQRQSTVTLQSHYDNLDDYHPVPQSQTTLANRGCSSSYHSPGFSTPHSNRAYSTALGQGAFIQAELSVQRPETEINAE

Target ORF information:

RefSeq Version	XM_009295411.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295411.3

ORF Insert Sequence:

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGT CTCTTTTCTC CCCTCAGGGG CCTGAGGCCT 
GGACGGCACC CTGAAGAAGA CGATATCGTG CCAGAGCTTG CCAGGAGCAT CCACCCCCGA 
GAGCGACCTG ACTGGGAGGA AACCATCAGC GCAATGGCAC GAAGTGCTGA AATTCCAGAG 
CTGAGCTCTG AGCCGCTCAT GCGCTCCTGC AGCAGCACCG CCAGTATGAA GGTGAAGAAT 
GTCAAAAAGC TGTCATTCAC CAAGGGCCAT TTTCCAAAGT TAGCTGAATG TGCCCACTTC 
CACTATGAAA ACGTTGATTT TGGAACAATA CAGTTAACCC TCGCAGATGA GCAGACTGAA 
GTGACACGGA ATGGCCTGGA GTCAAAAGAG CCTGTGTATT TGGTTCAGAT CTACTGTCAG 
GGACGAAGCT GGATCGTGAG ACGAAGCTAT GAAGATTTCC GAGTCTTGGA TAAGCATCTC 
CATCTCTGCA TCTATGACCG CCGATTTTCC CAGCTCCCTG AGCTGCCCCG TTTCGACAGC 
CTGAGGGAGA GAGCGGAGGC AGTTTCCCAG ATGCTGATGG CTTACCTCTC CCGTCTCTCA 
GCCATTGCTG ATAACAAGAT CAATTGTGGG CCGGCCCTCA CGTGGATGGA GGTTGACAAC 
AAAGGGAATC ATTTGCTGGT GCATGAAGAA TCATCTATAA ATGTTCCAGC TATCGCAGCG 
GCCCACGTGA TCAAGCGCTA CATCGCACAG GCAGCTGATG AGCTGTCATT TGAGGTGGGG 
GATATTGTTT CTGTCATTGA CATGCCACCC AAAGAGGACA CCACCTGGTG GAGGGGAAAA 
CATGGTTTCC AGGTTGGCTT CTTTCCCAGC GAGTGTGTGG AGCTGATAAA CGACAAGGTC 
CCGCAGTCTG TCACCAACTC TGTGCCAAAA CCAGCGTCCC CCTGCTCTGG TCTGCGGCCG 
GCATCCTGGA GTCTGTTCCC ACCCTATTTG GAGATGGAGA GTATAAAGCA GGACAGTGCA 
TTGGCACCCG ACCCATTAAA CCCCTACAGG CTGAGCTCAG TATCCAAGAA ACACGGGAAA 
CTGATCACTT TTCTGCGCAC ATTCATGAAG TCCAGGCCGA CCAAGCAGAA GCTGAAGCAA 
AGAGGTATCC TCAGGGAGAG GGTGTTCGGC TGTGACCTAG GAGAGCATCT CCTCAACTCT 
GGCCATGATG TCCCCCAGGT ACTCAAGAGC TGCACCGAGT TCATTGAGAA GCATGGAGTG 
GTTGATGGAA TGTATCGTCT CTCTGGGATC GCCTCCAACA TTCAGAAGCT GCGGCACGAG 
TTTGATTCTG AACAGATCCC AGATCTGACA AAAGATGTTT ACATTCAGGA TATACACTGC 
GTGGGCTCCC TGTGCAAGCT CTACTTTCGA GAGCTTCCCA ACCCACTTCT CACCTACCAG 
CTGTACGAGA AATTCTCGGA GGCTGTGTCA GCAGCCACAG ACGAGGAGAG GCTGATCAAA 
ATACATGACG TCATTCAGCA GCTGCCGCCT CCGCATTATA GAACCCTTGA GTTTCTTATG 
AGGCATCTTT CACACTTGGC AACCTTCAGC TATGTAACGA ACATGCATAC TAAAAATTTG 
GCCATCGTTT GGGCCCCCAA CTTACTGAGG TCAAAGCAAA TAGAATCTGC TTGCTTCAGT 
GGGACAGCAG CCTTCATGGA AGTACGAATA CAATCGGTTG TGGTGGAGTT CATCCTGAAT 
CATGTGGATG TTCTCTTCAG TCCCAAACTG AGCTCTCTTA TTCGAGAGGG AACAGGACAC 
AACTCTTTGT CTCGGCCTAA GTCTTTGCTA GTACCGTCTC CATCCACAAA GCTGCTAACT 
CTAGAAGAGG CACAGGCACG CACACAGGCC CAGATCAACT CACCCATCAC TGCAGACAGC 
AAGTACATAG AGGTCGGAGA GGGTCCTGCA GCATTACAAG GAAAATTCCA CACAGTCATA 
GAATTCCCTA CTGAGAGGAA GAGGCAGCCC ATGAAGTCTA AGAAGTCTCC AGTTGGCAGT 
TGGCGTTCTT TTTTCAACCT GGGCAAGTCT TCTTCAATGT CGAAACGCAA ACTACAGCGT 
AATCCCAGTG AACCAAATGA ACTCAAGGCT ATGGCACTGG CTGGAGGAAG AGGAGACACT 
GGTACTCTTC GGTCAGCCAA AAGTGAAGAG TCTCTCACCT CTCTACATAA TGTTGAAGGA 
GAGTCTAAAG TGTATAGGCC CCGCAGACCT CGCTCCAGCA GTGATGCCCT CTCTGCTTCA 
TTCAATGGTG ACCTTCTGGA TACCAGACAG CACTGTAACT CGTACGACAA CCTAGGTCAT 
GGAGACAGTG ATGGGGATGA TGGTCCAATC TGTGTGCCTG CTCTCATCTC CCCACCACGC 
TCCGCTGATG ATGTGGATCT GAGCCCCCCT GACATTGGCA TGGCCTCTTT AGACTTTGAC 
CCCATGTCTT TCCAATGTAG CCTTCCTCTG GCAGAAACTT CGATTTTCTC AATTGACGCA 
GATACAAGCA GTCTAAAAAG GAGCCCTGCC AGTGGCTCAG AGTCAGTCTC GCCAGTCCGA 
GCTAAAGTTA CGAGCCGGTT TTTGTCTCCA GACCACAGTA CAGCCTTAAA GGATAAACAT 
AAACTGCTCC TTACATCTTT CTCTGAGAAA ACTCCAGTTC AGTCTGTTGA AAGCACCGAG 
ATGTCAGCAG TTGTTTCTCC TCCAAGCATC TCTGAATCAG CTGCCTCCGT GATAATGAAA 
GGACCAGAGA GCACTGTGCT TCAGCCCTCT TCCCCTCCAT CTCCACTCGA CTCTCCTACA 
AAATTGAGCT CTCTGTCAAG AACCACCGAT CTGCCCCTGA GTGAAGCCAT TCAACAAGAG 
CTTCTTTCTA AAGCTGCTAC CTTTGAGAGT AAAGACATAT CGAGCATTGA ATGTTCACAA 
CCACCACAGG TCACCTGCTC TCAAGAACAG CCAGGAGCCG TAGCTTTGGA CTCTCCAAAG 
GGCAATGTCC CTGTCAGCTC TGTGTCCCTC GTCCCTCCCC CTCCCCCACC AAAGAACGCT 
GCCCGCATGC TTGCACTAGC TCTAGCTGAA TCTGCCCAGC AGGCCACCAT TCTTTCTCAG 
AAGCAATCCT CTGGACCCCC TACACCAGTA TCCCCTCTTA GGCCTCAAGA GTTATTGGAG 
ACCCTGGACT GGCCGCTGCC TTCTTCCTTG CCATCACAGC CATCCGAGGA GGAAACTGCA 
GTAGAATCAT CAAATAAATC GGTTCCTTTG TCTTCACCAC CCTCCACTCC ACCTGAAAAA 
ATGACTCGCT CAGTCAGTCT GCACCTCACC ACAGCTGATA GTGGGAAGCC ATCCAGCATT 
TCATGCAACA GCCAGGATGT AATCTCACCA GAGACCCCAA GCCAGAGTGC TATGCCCTCT 
TGTCTGACTT CTCATGTTTC TCAAACACAA AAAAGTCCAG AAAAACAGCA GCCTGCTGTC 
GGTCAGACAC CTGTGAGCAC AGCAAAAACC ACCACCACAA CCACCACTTC AACCATCAGC 
AGTAAGGATG CTGTCATTCA ACAACCCACC TCTGAGATCC ATATGCCTGA AACAGTCCTG 
CCACCAAAAC CAAACAAGGT GCCAGATCAA CCAGTGGCAA TTCTTCAGCC TCAGCCTCAA 
ATGCAGACGC AACCTCATAC TGCATTTCGG TCTCAACCTC AAGTTCAGCC TCTTCCTCAG 
TCACAAGCAC AGCCACAGTC TCAGCCCAGA TCACAGCCCA ATGCTAGAGT TGCACCAACC 
CTGGCAGCAC CTCATGATCC TGTGGAAAAA CCATGGGAAG CCATAAAGCC TGTCCATCCA 
AAAGGAGATA GTGCTTCTCA ACAACACCAC ACTCAGGGAG TGACACCACC AACACCCCCT 
ATTCGCTCGA TTGAAAGTAA ACTAGCAGCA GCTGCACTTT GCAACACCGA GGCTGCAAAC 
CCTGCCAACT TTCATGCCAT GTTAGAGACA TCCATGAGGG GATCAGTAGA GGAGACTCCC 
CCACAGTCTC ATCCGCCTCA CCGCAGATCG TCTACTCATC AGTCAGGTTA TCTTTATCAC 
ACAAAAGGGG AGGCAGCCTT ACTGGACCCC ACCACAGAGG CATATTACCA TCAGCGGGTA 
ACCCCTTCAG GTCAGGCTAC AGTGGGGTAC CATTACCGGC CAGAGAGTGT TCCACCTCAC 
ATTTCTTACG TGTCAAAATC AGAGCCACAC ATTTCCTACA GAGGTCATGG GGATGGTCGA 
TACAATACAA TTGGACCTAG ATCCTACCAC CAGTCTATCA AATCTCGTGG TTCACTTAGA 
AGTGAGTACA TCTCTCCAAG CTCATTACAC CACTCCCATA GCTACAGCCA AGCAGATGGA 
CCAACCGTCT ACCCAACAAT TCGCAGGGTC CACTCCTTAC ATGTGCCTCC CACTGCTATT 
CGCTCAATGC CAGTCACCAG AACTGAGGTA CCACCAGATG ATGAGTTATT TTACTACCAG 
CGCCCAATCT ACCACTGCAA GTCTCACCAA CCATCATCTC AAGTCGACTA TCACGTCACA 
CAACTGCAGC CTTATTTTGA AAATGGAAGA GTTCAGTACC GCTACAGCCC CTACTCTGGC 
TCTCACGTTC TGGAATCCCC TTTCTATGAT GTGGACCATT ATGGTACTAT CCGCTTAAGG 
CACGTCCATT CCTTCAGTGG TCGGGACAGT GCCCCTCTCC TTCGATCTGG AGCAAAATCT 
GGAGGCTACC ACTACCTTTC ACGGCATGTT ATTCCTTTAA AGGAGCATGG CTTCATAAGC 
AGGGACATGC CACCTTACAG TGGTTCAAAA GGGAGTATTT ACTTTGCATG GGACCCAGAG 
GAGGCAGAAA GGCTGCGCAT ACACTCCATC CGTAGGGAAA GCAGAGCAAG GCAAAAGGTT 
AAGGGTTCTG TTATGTCTCA ATACGACAAT GTAGGACCCT ACATGCCTGT GGATGTTGAC 
ATGTTACATT TGCGCAGCAA ATCAGATCCT GGAAAAACTG TATTGGTGTC CTCGGAGAGC 
AAAGAGGCCA GATACAATGT TGTGCCTGGA TCTCAGCATG CTCCCAGACA CTTGATCTCT 
GATCCAGATG TCCTAATGTA TATGGAAACA GAGAAGCACT GCCAAGGAAA TGGAATGGGG 
GAGAAAACCA CCAAGCAAGG CAGCTCTAGG ACCTACTCGT CTATACATTC ACATCAATCC 
CAGCTTCCAC CACTAAGCCG GCAGCACTTA CCAGAGGGTG GCCATCATGA CCCAAAGTTT 
GAACCAGGGG AAAAGCAACT GAGTGGTAAA CATTGGCAGG AACATTCGGA CAGCAGGAGT 
GCTCAAGCAC GTTATGAAAG GTCTGATTTG GATCGCCACA TATGCAGGGT GAAGGCCACT 
AGCAGTGCCA GTGAAGATGA ACAAGCTGCT CCTGTGAAGC CGGCTCCTCC ACCTAAGCCC 
GAGAGATCCC ATAGTGTTCG AGAGCGGCAC CATTACAACC AGTCCAGTCT TCCTGCATAT 
TTACCAGACA ATTCAGATAG AGACCACAGT GGAACATACT CCCATCAGTC ACAGGGGCAG 
AGACAAAGCA CCGTTACTTT GCAGTCGCAT TACGACAACC TGGATGACTA TCATCCAGTA 
CCTCAATCCC AAACCACGCT AGCAAATCGG GGTTGTTCCA GTTCCTATCA TAGCCCCGGT 
TTCTCAACTC CACACAGTAA TCGTGCATAC TCCACTGCCT TGGGACAGGG TGCCTTCATA 
CAAGCTGAGC TTTCAGTGCA AAGGCCAGAG ACTGAGATAA ATGCTGAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32b ( XM_021469079.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324754.1 Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X5, mRNA.

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CloneID	ODa45740
Clone ID Related Accession (Same CDS sequence)	XM_021469079.1 , XM_009295413.2 , XM_009295413.3
Accession Version	XM_021469079.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho GTPase-activating protein 32 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGG GGCCTGAGGC CTGGACGGCA CCCTGAAGAA 
GACGATATCG TGCCAGAGCT TGCCAGGAGC ATCCACCCCC GAGAGCGACC TGACTGGGAG 
GAAACCATCA GCGCAATGGC ACGAAGTGCT GAAATTCCAG AGCTGAGCTC TGAGCCGCTC 
ATGCGCTCCT GCAGCAGCAC CGCCAGTATG AAGGTGAAGA ATGTCAAAAA GCTGTCATTC 
ACCAAGGGCC ATTTTCCAAA GTTAGCTGAA TGTGCCCACT TCCACTATGA AAACGTTGAT 
TTTGGAACAA TACAGTTAAC CCTCGCAGAT GAGCAGACTG AAGTGACACG GAATGGCCTG 
GAGTCAAAAG AGCCTGTGTA TTTGGTTCAG ATCTACTGTC AGGGACGAAG CTGGATCGTG 
AGACGAAGCT ATGAAGATTT CCGAGTCTTG GATAAGCATC TCCATCTCTG CATCTATGAC 
CGCCGATTTT CCCAGCTCCC TGAGCTGCCC CGTTTCGACA GCCTGAGGGA GAGAGCGGAG 
GCAGTTTCCC AGATGCTGAT GGCTTACCTC TCCCGTCTCT CAGCCATTGC TGATAACAAG 
ATCAATTGTG GGCCGGCCCT CACGTGGATG GAGGTTGACA ACAAAGGGAA TCATTTGCTG 
GTGCATGAAG AATCATCTAT AAATGTTCCA GCTATCGCAG CGGCCCACGT GATCAAGCGC 
TACATCGCAC AGGCAGCTGA TGAGCTGTCA TTTGAGGTGG GGGATATTGT TTCTGTCATT 
GACATGCCAC CCAAAGAGGA CACCACCTGG TGGAGGGGAA AACATGGTTT CCAGGTTGGC 
TTCTTTCCCA GCGAGTGTGT GGAGCTGATA AACGACAAGG TCCCGCAGTC TGTCACCAAC 
TCTGTGCCAA AACCAGCGTC CCCCTGCTCT GGTCTGCGGC CGGCATCCTG GAGTCTGTTC 
CCACCCTATT TGGAGATGGA GAGTATAAAG CAGGACAGTG CATTGGCACC CGACCCATTA 
AACCCCTACA GGCTGAGCTC AGTATCCAAG AAACACGGGA AACTGATCAC TTTTCTGCGC 
ACATTCATGA AGTCCAGGCC GACCAAGCAG AAGCTGAAGC AAAGAGGTAT CCTCAGGGAG 
AGGGTGTTCG GCTGTGACCT AGGAGAGCAT CTCCTCAACT CTGGCCATGA TGTCCCCCAG 
GTACTCAAGA GCTGCACCGA GTTCATTGAG AAGCATGGAG TGGTTGATGG AATGTATCGT 
CTCTCTGGGA TCGCCTCCAA CATTCAGAAG CTGCGGCACG AGTTTGATTC TGAACAGATC 
CCAGATCTGA CAAAAGATGT TTACATTCAG GATATACACT GCGTGGGCTC CCTGTGCAAG 
CTCTACTTTC GAGAGCTTCC CAACCCACTT CTCACCTACC AGCTGTACGA GAAATTCTCG 
GAGGCTGTGT CAGCAGCCAC AGACGAGGAG AGGCTGATCA AAATACATGA CGTCATTCAG 
CAGCTGCCGC CTCCGCATTA TAGAACCCTT GAGTTTCTTA TGAGGCATCT TTCACACTTG 
GCAACCTTCA GCTATGTAAC GAACATGCAT ACTAAAAATT TGGCCATCGT TTGGGCCCCC 
AACTTACTGA GGTCAAAGCA AATAGAATCT GCTTGCTTCA GTGGGACAGC AGCCTTCATG 
GAAGTACGAA TACAATCGGT TGTGGTGGAG TTCATCCTGA ATCATGTGGA TGTTCTCTTC 
AGTCCCAAAC TGAGCTCTCT TATTCGAGAG GGAACAGGAC ACAACTCTTT GTCTCGGCCT 
AAGTCTTTGC TAGTACCGTC TCCATCCACA AAGCTGCTAA CTCTAGAAGA GGCACAGGCA 
CGCACACAGG CCCAGATCAA CTCACCCATC ACTGCAGACA GCAAGTACAT AGAGGTCGGA 
GAGGGTCCTG CAGCATTACA AGGAAAATTC CACACAGTCA TAGAATTCCC TACTGAGAGG 
AAGAGGCAGC CCATGAAGTC TAAGAAGTCT CCAGTTGGCA GTTGGCGTTC TTTTTTCAAC 
CTGGGCAAGT CTTCTTCAAT GTCGAAACGC AAACTACAGC GTAATCCCAG TGAACCAAAT 
GAACTCAAGG CTATGGCACT GGCTGGAGGA AGAGGAGACA CTGGTACTCT TCGGTCAGCC 
AAAAGTGAAG AGTCTCTCAC CTCTCTACAT AATGTTGAAG GAGAGTCTAA AGTGTATAGG 
CCCCGCAGAC CTCGCTCCAG CAGTGATGCC CTCTCTGCTT CATTCAATGG TGACCTTCTG 
GATACCAGAC AGCACTGTAA CTCGTACGAC AACCTAGGTC ATGGAGACAG TGATGGGGAT 
GATGGTCCAA TCTGTGTGCC TGCTCTCATC TCCCCACCAC GCTCCGCTGA TGATGTGGAT 
CTGAGCCCCC CTGACATTGG CATGGCCTCT TTAGACTTTG ACCCCATGTC TTTCCAATGT 
AGCCTTCCTC TGGCAGAAAC TTCGATTTTC TCAATTGACG CAGATACAAG CAGTCTAAAA 
AGGAGCCCTG CCAGTGGCTC AGAGTCAGTC TCGCCAGTCC GAGCTAAAGT TACGAGCCGG 
TTTTTGTCTC CAGACCACAG TACAGCCTTA AAGGATAAAC ATAAACTGCT CCTTACATCT 
TTCTCTGAGA AAACTCCAGT TCAGTCTGTT GAAAGCACCG AGATGTCAGC AGTTGTTTCT 
CCTCCAAGCA TCTCTGAATC AGCTGCCTCC GTGATAATGA AAGGACCAGA GAGCACTGTG 
CTTCAGCCCT CTTCCCCTCC ATCTCCACTC GACTCTCCTA CAAAATTGAG CTCTCTGTCA 
AGAACCACCG ATCTGCCCCT GAGTGAAGCC ATTCAACAAG AGCTTCTTTC TAAAGCTGCT 
ACCTTTGAGA GTAAAGACAT ATCGAGCATT GAATGTTCAC AACCACCACA GGTCACCTGC 
TCTCAAGAAC AGCCAGGAGC CGTAGCTTTG GACTCTCCAA AGGGCAATGT CCCTGTCAGC 
TCTGTGTCCC TCGTCCCTCC CCCTCCCCCA CCAAAGAACG CTGCCCGCAT GCTTGCACTA 
GCTCTAGCTG AATCTGCCCA GCAGGCCACC ATTCTTTCTC AGAAGCAATC CTCTGGACCC 
CCTACACCAG TATCCCCTCT TAGGCCTCAA GAGTTATTGG AGACCCTGGA CTGGCCGCTG 
CCTTCTTCCT TGCCATCACA GCCATCCGAG GAGGAAACTG CAGTAGAATC ATCAAATAAA 
TCGGTTCCTT TGTCTTCACC ACCCTCCACT CCACCTGAAA AAATGACTCG CTCAGTCAGT 
CTGCACCTCA CCACAGCTGA TAGTGGGAAG CCATCCAGCA TTTCATGCAA CAGCCAGGAT 
GTAATCTCAC CAGAGACCCC AAGCCAGAGT GCTATGCCCT CTTGTCTGAC TTCTCATGTT 
TCTCAAACAC AAAAAAGTCC AGAAAAACAG CAGCCTGCTG TCGGTCAGAC ACCTGTGAGC 
ACAGCAAAAA CCACCACCAC AACCACCACT TCAACCATCA GCAGTAAGGA TGCTGTCATT 
CAACAACCCA CCTCTGAGAT CCATATGCCT GAAACAGTCC TGCCACCAAA ACCAAACAAG 
GTGCCAGATC AACCAGTGGC AATTCTTCAG CCTCAGCCTC AAATGCAGAC GCAACCTCAT 
ACTGCATTTC GGTCTCAACC TCAAGTTCAG CCTCTTCCTC AGTCACAAGC ACAGCCACAG 
TCTCAGCCCA GATCACAGCC CAATGCTAGA GTTGCACCAA CCCTGGCAGC ACCTCATGAT 
CCTGTGGAAA AACCATGGGA AGCCATAAAG CCTGTCCATC CAAAAGGAGA TAGTGCTTCT 
CAACAACACC ACACTCAGGG AGTGACACCA CCAACACCCC CTATTCGCTC GATTGAAAGT 
AAACTAGCAG CAGCTGCACT TTGCAACACC GAGGCTGCAA ACCCTGCCAA CTTTCATGCC 
ATGTTAGAGA CATCCATGAG GGGATCAGTA GAGGAGACTC CCCCACAGTC TCATCCGCCT 
CACCGCAGAT CGTCTACTCA TCAGTCAGGT TATCTTTATC ACACAAAAGG GGAGGCAGCC 
TTACTGGACC CCACCACAGA GGCATATTAC CATCAGCGGG TAACCCCTTC AGGTCAGGCT 
ACAGTGGGGT ACCATTACCG GCCAGAGAGT GTTCCACCTC ACATTTCTTA CGTGTCAAAA 
TCAGAGCCAC ACATTTCCTA CAGAGGTCAT GGGGATGGTC GATACAATAC AATTGGACCT 
AGATCCTACC ACCAGTCTAT CAAATCTCGT GGTTCACTTA GAAGTGAGTA CATCTCTCCA 
AGCTCATTAC ACCACTCCCA TAGCTACAGC CAAGCAGATG GACCAACCGT CTACCCAACA 
ATTCGCAGGG TCCACTCCTT ACATGTGCCT CCCACTGCTA TTCGCTCAAT GCCAGTCACC 
AGAACTGAGG TACCACCAGA TGATGAGTTA TTTTACTACC AGCGCCCAAT CTACCACTGC 
AAGTCTCACC AACCATCATC TCAAGTCGAC TATCACGTCA CACAACTGCA GCCTTATTTT 
GAAAATGGAA GAGTTCAGTA CCGCTACAGC CCCTACTCTG GCTCTCACGT TCTGGAATCC 
CCTTTCTATG ATGTGGACCA TTATGGTACT ATCCGCTTAA GGCACGTCCA TTCCTTCAGT 
GGTCGGGACA GTGCCCCTCT CCTTCGATCT GGAGCAAAAT CTGGAGGCTA CCACTACCTT 
TCACGGCATG TTATTCCTTT AAAGGAGCAT GGCTTCATAA GCAGGGACAT GCCACCTTAC 
AGTGGTTCAA AAGGGAGTAT TTACTTTGCA TGGGACCCAG AGGAGGCAGA AAGGCTGCGC 
ATACACTCCA TCCGTAGGGA AAGCAGAGCA AGGCAAAAGG TTAAGGGTTC TGTTATGTCT 
CAATACGACA ATGTAGGACC CTACATGCCT GTGGATGTTG ACATGTTACA TTTGCGCAGC 
AAATCAGATC CTGGAAAAAC TGTATTGGTG TCCTCGGAGA GCAAAGAGGC CAGATACAAT 
GTTGTGCCTG GATCTCAGCA TGCTCCCAGA CACTTGATCT CTGATCCAGA TGTCCTAATG 
TATATGGAAA CAGAGAAGCA CTGCCAAGGA AATGGAATGG GGGAGAAAAC CACCAAGCAA 
GGCAGCTCTA GGACCTACTC GTCTATACAT TCACATCAAT CCCAGCTTCC ACCACTAAGC 
CGGCAGCACT TACCAGAGGG TGGCCATCAT GACCCAAAGT TTGAACCAGG GGAAAAGCAA 
CTGAGTGGTA AACATTGGCA GGAACATTCG GACAGCAGGA GTGCTCAAGC ACGTTATGAA 
AGGTCTGATT TGGATCGCCA CATATGCAGG GTGAAGGCCA CTAGCAGTGC CAGTGAAGAT 
GAACAAGCTG CTCCTGTGAA GCCGGCTCCT CCACCTAAGC CCGAGAGATC CCATAGTGTT 
CGAGAGCGGC ACCATTACAA CCAGTCCAGT CTTCCTGCAT ATTTACCAGA CAATTCAGAT 
AGAGACCACA GTGGAACATA CTCCCATCAG TCACAGGGGC AGAGACAAAG CACCGTTACT 
TTGCAGTCGC ATTACGACAA CCTGGATGAC TATCATCCAG TACCTCAATC CCAAACCACG 
CTAGCAAATC GGGGTTGTTC CAGTTCCTAT CATAGCCCCG GTTTCTCAAC TCCACACAGT 
AATCGTGCAT ACTCCACTGC CTTGGGACAG GGTGCCTTCA TACAAGCTGA GCTTTCAGTG 
CAAAGGCCAG AGACTGAGAT AAATGCTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324754.1
CDS	707..6559
Translation	MEAGSGAAAVVGSAALGLLGATGSHDISDRGLRPGRHPEEDDIVPELARSIHPRERPDWEETISAMARSAEIPELSSEPLMRSCSSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGTIQLTLADEQTEVTRNGLESKEPVYLVQIYCQGRSWIVRRSYEDFRVLDKHLHLCIYDRRFSQLPELPRFDSLRERAEAVSQMLMAYLSRLSAIADNKINCGPALTWMEVDNKGNHLLVHEESSINVPAIAAAHVIKRYIAQAADELSFEVGDIVSVIDMPPKEDTTWWRGKHGFQVGFFPSECVELINDKVPQSVTNSVPKPASPCSGLRPASWSLFPPYLEMESIKQDSALAPDPLNPYRLSSVSKKHGKLITFLRTFMKSRPTKQKLKQRGILRERVFGCDLGEHLLNSGHDVPQVLKSCTEFIEKHGVVDGMYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSEAVSAATDEERLIKIHDVIQQLPPPHYRTLEFLMRHLSHLATFSYVTNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVDVLFSPKLSSLIREGTGHNSLSRPKSLLVPSPSTKLLTLEEAQARTQAQINSPITADSKYIEVGEGPAALQGKFHTVIEFPTERKRQPMKSKKSPVGSWRSFFNLGKSSSMSKRKLQRNPSEPNELKAMALAGGRGDTGTLRSAKSEESLTSLHNVEGESKVYRPRRPRSSSDALSASFNGDLLDTRQHCNSYDNLGHGDSDGDDGPICVPALISPPRSADDVDLSPPDIGMASLDFDPMSFQCSLPLAETSIFSIDADTSSLKRSPASGSESVSPVRAKVTSRFLSPDHSTALKDKHKLLLTSFSEKTPVQSVESTEMSAVVSPPSISESAASVIMKGPESTVLQPSSPPSPLDSPTKLSSLSRTTDLPLSEAIQQELLSKAATFESKDISSIECSQPPQVTCSQEQPGAVALDSPKGNVPVSSVSLVPPPPPPKNAARMLALALAESAQQATILSQKQSSGPPTPVSPLRPQELLETLDWPLPSSLPSQPSEEETAVESSNKSVPLSSPPSTPPEKMTRSVSLHLTTADSGKPSSISCNSQDVISPETPSQSAMPSCLTSHVSQTQKSPEKQQPAVGQTPVSTAKTTTTTTTSTISSKDAVIQQPTSEIHMPETVLPPKPNKVPDQPVAILQPQPQMQTQPHTAFRSQPQVQPLPQSQAQPQSQPRSQPNARVAPTLAAPHDPVEKPWEAIKPVHPKGDSASQQHHTQGVTPPTPPIRSIESKLAAAALCNTEAANPANFHAMLETSMRGSVEETPPQSHPPHRRSSTHQSGYLYHTKGEAALLDPTTEAYYHQRVTPSGQATVGYHYRPESVPPHISYVSKSEPHISYRGHGDGRYNTIGPRSYHQSIKSRGSLRSEYISPSSLHHSHSYSQADGPTVYPTIRRVHSLHVPPTAIRSMPVTRTEVPPDDELFYYQRPIYHCKSHQPSSQVDYHVTQLQPYFENGRVQYRYSPYSGSHVLESPFYDVDHYGTIRLRHVHSFSGRDSAPLLRSGAKSGGYHYLSRHVIPLKEHGFISRDMPPYSGSKGSIYFAWDPEEAERLRIHSIRRESRARQKVKGSVMSQYDNVGPYMPVDVDMLHLRSKSDPGKTVLVSSESKEARYNVVPGSQHAPRHLISDPDVLMYMETEKHCQGNGMGEKTTKQGSSRTYSSIHSHQSQLPPLSRQHLPEGGHHDPKFEPGEKQLSGKHWQEHSDSRSAQARYERSDLDRHICRVKATSSASEDEQAAPVKPAPPPKPERSHSVRERHHYNQSSLPAYLPDNSDRDHSGTYSHQSQGQRQSTVTLQSHYDNLDDYHPVPQSQTTLANRGCSSSYHSPGFSTPHSNRAYSTALGQGAFIQAELSVQRPETEINAE

Target ORF information:

RefSeq Version	XM_021469079.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469079.1

ORF Insert Sequence:

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGG GGCCTGAGGC CTGGACGGCA CCCTGAAGAA 
GACGATATCG TGCCAGAGCT TGCCAGGAGC ATCCACCCCC GAGAGCGACC TGACTGGGAG 
GAAACCATCA GCGCAATGGC ACGAAGTGCT GAAATTCCAG AGCTGAGCTC TGAGCCGCTC 
ATGCGCTCCT GCAGCAGCAC CGCCAGTATG AAGGTGAAGA ATGTCAAAAA GCTGTCATTC 
ACCAAGGGCC ATTTTCCAAA GTTAGCTGAA TGTGCCCACT TCCACTATGA AAACGTTGAT 
TTTGGAACAA TACAGTTAAC CCTCGCAGAT GAGCAGACTG AAGTGACACG GAATGGCCTG 
GAGTCAAAAG AGCCTGTGTA TTTGGTTCAG ATCTACTGTC AGGGACGAAG CTGGATCGTG 
AGACGAAGCT ATGAAGATTT CCGAGTCTTG GATAAGCATC TCCATCTCTG CATCTATGAC 
CGCCGATTTT CCCAGCTCCC TGAGCTGCCC CGTTTCGACA GCCTGAGGGA GAGAGCGGAG 
GCAGTTTCCC AGATGCTGAT GGCTTACCTC TCCCGTCTCT CAGCCATTGC TGATAACAAG 
ATCAATTGTG GGCCGGCCCT CACGTGGATG GAGGTTGACA ACAAAGGGAA TCATTTGCTG 
GTGCATGAAG AATCATCTAT AAATGTTCCA GCTATCGCAG CGGCCCACGT GATCAAGCGC 
TACATCGCAC AGGCAGCTGA TGAGCTGTCA TTTGAGGTGG GGGATATTGT TTCTGTCATT 
GACATGCCAC CCAAAGAGGA CACCACCTGG TGGAGGGGAA AACATGGTTT CCAGGTTGGC 
TTCTTTCCCA GCGAGTGTGT GGAGCTGATA AACGACAAGG TCCCGCAGTC TGTCACCAAC 
TCTGTGCCAA AACCAGCGTC CCCCTGCTCT GGTCTGCGGC CGGCATCCTG GAGTCTGTTC 
CCACCCTATT TGGAGATGGA GAGTATAAAG CAGGACAGTG CATTGGCACC CGACCCATTA 
AACCCCTACA GGCTGAGCTC AGTATCCAAG AAACACGGGA AACTGATCAC TTTTCTGCGC 
ACATTCATGA AGTCCAGGCC GACCAAGCAG AAGCTGAAGC AAAGAGGTAT CCTCAGGGAG 
AGGGTGTTCG GCTGTGACCT AGGAGAGCAT CTCCTCAACT CTGGCCATGA TGTCCCCCAG 
GTACTCAAGA GCTGCACCGA GTTCATTGAG AAGCATGGAG TGGTTGATGG AATGTATCGT 
CTCTCTGGGA TCGCCTCCAA CATTCAGAAG CTGCGGCACG AGTTTGATTC TGAACAGATC 
CCAGATCTGA CAAAAGATGT TTACATTCAG GATATACACT GCGTGGGCTC CCTGTGCAAG 
CTCTACTTTC GAGAGCTTCC CAACCCACTT CTCACCTACC AGCTGTACGA GAAATTCTCG 
GAGGCTGTGT CAGCAGCCAC AGACGAGGAG AGGCTGATCA AAATACATGA CGTCATTCAG 
CAGCTGCCGC CTCCGCATTA TAGAACCCTT GAGTTTCTTA TGAGGCATCT TTCACACTTG 
GCAACCTTCA GCTATGTAAC GAACATGCAT ACTAAAAATT TGGCCATCGT TTGGGCCCCC 
AACTTACTGA GGTCAAAGCA AATAGAATCT GCTTGCTTCA GTGGGACAGC AGCCTTCATG 
GAAGTACGAA TACAATCGGT TGTGGTGGAG TTCATCCTGA ATCATGTGGA TGTTCTCTTC 
AGTCCCAAAC TGAGCTCTCT TATTCGAGAG GGAACAGGAC ACAACTCTTT GTCTCGGCCT 
AAGTCTTTGC TAGTACCGTC TCCATCCACA AAGCTGCTAA CTCTAGAAGA GGCACAGGCA 
CGCACACAGG CCCAGATCAA CTCACCCATC ACTGCAGACA GCAAGTACAT AGAGGTCGGA 
GAGGGTCCTG CAGCATTACA AGGAAAATTC CACACAGTCA TAGAATTCCC TACTGAGAGG 
AAGAGGCAGC CCATGAAGTC TAAGAAGTCT CCAGTTGGCA GTTGGCGTTC TTTTTTCAAC 
CTGGGCAAGT CTTCTTCAAT GTCGAAACGC AAACTACAGC GTAATCCCAG TGAACCAAAT 
GAACTCAAGG CTATGGCACT GGCTGGAGGA AGAGGAGACA CTGGTACTCT TCGGTCAGCC 
AAAAGTGAAG AGTCTCTCAC CTCTCTACAT AATGTTGAAG GAGAGTCTAA AGTGTATAGG 
CCCCGCAGAC CTCGCTCCAG CAGTGATGCC CTCTCTGCTT CATTCAATGG TGACCTTCTG 
GATACCAGAC AGCACTGTAA CTCGTACGAC AACCTAGGTC ATGGAGACAG TGATGGGGAT 
GATGGTCCAA TCTGTGTGCC TGCTCTCATC TCCCCACCAC GCTCCGCTGA TGATGTGGAT 
CTGAGCCCCC CTGACATTGG CATGGCCTCT TTAGACTTTG ACCCCATGTC TTTCCAATGT 
AGCCTTCCTC TGGCAGAAAC TTCGATTTTC TCAATTGACG CAGATACAAG CAGTCTAAAA 
AGGAGCCCTG CCAGTGGCTC AGAGTCAGTC TCGCCAGTCC GAGCTAAAGT TACGAGCCGG 
TTTTTGTCTC CAGACCACAG TACAGCCTTA AAGGATAAAC ATAAACTGCT CCTTACATCT 
TTCTCTGAGA AAACTCCAGT TCAGTCTGTT GAAAGCACCG AGATGTCAGC AGTTGTTTCT 
CCTCCAAGCA TCTCTGAATC AGCTGCCTCC GTGATAATGA AAGGACCAGA GAGCACTGTG 
CTTCAGCCCT CTTCCCCTCC ATCTCCACTC GACTCTCCTA CAAAATTGAG CTCTCTGTCA 
AGAACCACCG ATCTGCCCCT GAGTGAAGCC ATTCAACAAG AGCTTCTTTC TAAAGCTGCT 
ACCTTTGAGA GTAAAGACAT ATCGAGCATT GAATGTTCAC AACCACCACA GGTCACCTGC 
TCTCAAGAAC AGCCAGGAGC CGTAGCTTTG GACTCTCCAA AGGGCAATGT CCCTGTCAGC 
TCTGTGTCCC TCGTCCCTCC CCCTCCCCCA CCAAAGAACG CTGCCCGCAT GCTTGCACTA 
GCTCTAGCTG AATCTGCCCA GCAGGCCACC ATTCTTTCTC AGAAGCAATC CTCTGGACCC 
CCTACACCAG TATCCCCTCT TAGGCCTCAA GAGTTATTGG AGACCCTGGA CTGGCCGCTG 
CCTTCTTCCT TGCCATCACA GCCATCCGAG GAGGAAACTG CAGTAGAATC ATCAAATAAA 
TCGGTTCCTT TGTCTTCACC ACCCTCCACT CCACCTGAAA AAATGACTCG CTCAGTCAGT 
CTGCACCTCA CCACAGCTGA TAGTGGGAAG CCATCCAGCA TTTCATGCAA CAGCCAGGAT 
GTAATCTCAC CAGAGACCCC AAGCCAGAGT GCTATGCCCT CTTGTCTGAC TTCTCATGTT 
TCTCAAACAC AAAAAAGTCC AGAAAAACAG CAGCCTGCTG TCGGTCAGAC ACCTGTGAGC 
ACAGCAAAAA CCACCACCAC AACCACCACT TCAACCATCA GCAGTAAGGA TGCTGTCATT 
CAACAACCCA CCTCTGAGAT CCATATGCCT GAAACAGTCC TGCCACCAAA ACCAAACAAG 
GTGCCAGATC AACCAGTGGC AATTCTTCAG CCTCAGCCTC AAATGCAGAC GCAACCTCAT 
ACTGCATTTC GGTCTCAACC TCAAGTTCAG CCTCTTCCTC AGTCACAAGC ACAGCCACAG 
TCTCAGCCCA GATCACAGCC CAATGCTAGA GTTGCACCAA CCCTGGCAGC ACCTCATGAT 
CCTGTGGAAA AACCATGGGA AGCCATAAAG CCTGTCCATC CAAAAGGAGA TAGTGCTTCT 
CAACAACACC ACACTCAGGG AGTGACACCA CCAACACCCC CTATTCGCTC GATTGAAAGT 
AAACTAGCAG CAGCTGCACT TTGCAACACC GAGGCTGCAA ACCCTGCCAA CTTTCATGCC 
ATGTTAGAGA CATCCATGAG GGGATCAGTA GAGGAGACTC CCCCACAGTC TCATCCGCCT 
CACCGCAGAT CGTCTACTCA TCAGTCAGGT TATCTTTATC ACACAAAAGG GGAGGCAGCC 
TTACTGGACC CCACCACAGA GGCATATTAC CATCAGCGGG TAACCCCTTC AGGTCAGGCT 
ACAGTGGGGT ACCATTACCG GCCAGAGAGT GTTCCACCTC ACATTTCTTA CGTGTCAAAA 
TCAGAGCCAC ACATTTCCTA CAGAGGTCAT GGGGATGGTC GATACAATAC AATTGGACCT 
AGATCCTACC ACCAGTCTAT CAAATCTCGT GGTTCACTTA GAAGTGAGTA CATCTCTCCA 
AGCTCATTAC ACCACTCCCA TAGCTACAGC CAAGCAGATG GACCAACCGT CTACCCAACA 
ATTCGCAGGG TCCACTCCTT ACATGTGCCT CCCACTGCTA TTCGCTCAAT GCCAGTCACC 
AGAACTGAGG TACCACCAGA TGATGAGTTA TTTTACTACC AGCGCCCAAT CTACCACTGC 
AAGTCTCACC AACCATCATC TCAAGTCGAC TATCACGTCA CACAACTGCA GCCTTATTTT 
GAAAATGGAA GAGTTCAGTA CCGCTACAGC CCCTACTCTG GCTCTCACGT TCTGGAATCC 
CCTTTCTATG ATGTGGACCA TTATGGTACT ATCCGCTTAA GGCACGTCCA TTCCTTCAGT 
GGTCGGGACA GTGCCCCTCT CCTTCGATCT GGAGCAAAAT CTGGAGGCTA CCACTACCTT 
TCACGGCATG TTATTCCTTT AAAGGAGCAT GGCTTCATAA GCAGGGACAT GCCACCTTAC 
AGTGGTTCAA AAGGGAGTAT TTACTTTGCA TGGGACCCAG AGGAGGCAGA AAGGCTGCGC 
ATACACTCCA TCCGTAGGGA AAGCAGAGCA AGGCAAAAGG TTAAGGGTTC TGTTATGTCT 
CAATACGACA ATGTAGGACC CTACATGCCT GTGGATGTTG ACATGTTACA TTTGCGCAGC 
AAATCAGATC CTGGAAAAAC TGTATTGGTG TCCTCGGAGA GCAAAGAGGC CAGATACAAT 
GTTGTGCCTG GATCTCAGCA TGCTCCCAGA CACTTGATCT CTGATCCAGA TGTCCTAATG 
TATATGGAAA CAGAGAAGCA CTGCCAAGGA AATGGAATGG GGGAGAAAAC CACCAAGCAA 
GGCAGCTCTA GGACCTACTC GTCTATACAT TCACATCAAT CCCAGCTTCC ACCACTAAGC 
CGGCAGCACT TACCAGAGGG TGGCCATCAT GACCCAAAGT TTGAACCAGG GGAAAAGCAA 
CTGAGTGGTA AACATTGGCA GGAACATTCG GACAGCAGGA GTGCTCAAGC ACGTTATGAA 
AGGTCTGATT TGGATCGCCA CATATGCAGG GTGAAGGCCA CTAGCAGTGC CAGTGAAGAT 
GAACAAGCTG CTCCTGTGAA GCCGGCTCCT CCACCTAAGC CCGAGAGATC CCATAGTGTT 
CGAGAGCGGC ACCATTACAA CCAGTCCAGT CTTCCTGCAT ATTTACCAGA CAATTCAGAT 
AGAGACCACA GTGGAACATA CTCCCATCAG TCACAGGGGC AGAGACAAAG CACCGTTACT 
TTGCAGTCGC ATTACGACAA CCTGGATGAC TATCATCCAG TACCTCAATC CCAAACCACG 
CTAGCAAATC GGGGTTGTTC CAGTTCCTAT CATAGCCCCG GTTTCTCAAC TCCACACAGT 
AATCGTGCAT ACTCCACTGC CTTGGGACAG GGTGCCTTCA TACAAGCTGA GCTTTCAGTG 
CAAAGGCCAG AGACTGAGAT AAATGCTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32b ( XM_009295413.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293688.1 Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X4, mRNA.

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CloneID	ODa45740
Clone ID Related Accession (Same CDS sequence)	XM_021469079.1 , XM_009295413.2 , XM_009295413.3
Accession Version	XM_009295413.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho GTPase-activating protein 32 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295413.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGG GGCCTGAGGC CTGGACGGCA CCCTGAAGAA 
GACGATATCG TGCCAGAGCT TGCCAGGAGC ATCCACCCCC GAGAGCGACC TGACTGGGAG 
GAAACCATCA GCGCAATGGC ACGAAGTGCT GAAATTCCAG AGCTGAGCTC TGAGCCGCTC 
ATGCGCTCCT GCAGCAGCAC CGCCAGTATG AAGGTGAAGA ATGTCAAAAA GCTGTCATTC 
ACCAAGGGCC ATTTTCCAAA GTTAGCTGAA TGTGCCCACT TCCACTATGA AAACGTTGAT 
TTTGGAACAA TACAGTTAAC CCTCGCAGAT GAGCAGACTG AAGTGACACG GAATGGCCTG 
GAGTCAAAAG AGCCTGTGTA TTTGGTTCAG ATCTACTGTC AGGGACGAAG CTGGATCGTG 
AGACGAAGCT ATGAAGATTT CCGAGTCTTG GATAAGCATC TCCATCTCTG CATCTATGAC 
CGCCGATTTT CCCAGCTCCC TGAGCTGCCC CGTTTCGACA GCCTGAGGGA GAGAGCGGAG 
GCAGTTTCCC AGATGCTGAT GGCTTACCTC TCCCGTCTCT CAGCCATTGC TGATAACAAG 
ATCAATTGTG GGCCGGCCCT CACGTGGATG GAGGTTGACA ACAAAGGGAA TCATTTGCTG 
GTGCATGAAG AATCATCTAT AAATGTTCCA GCTATCGCAG CGGCCCACGT GATCAAGCGC 
TACATCGCAC AGGCAGCTGA TGAGCTGTCA TTTGAGGTGG GGGATATTGT TTCTGTCATT 
GACATGCCAC CCAAAGAGGA CACCACCTGG TGGAGGGGAA AACATGGTTT CCAGGTTGGC 
TTCTTTCCCA GCGAGTGTGT GGAGCTGATA AACGACAAGG TCCCGCAGTC TGTCACCAAC 
TCTGTGCCAA AACCAGCGTC CCCCTGCTCT GGTCTGCGGC CGGCATCCTG GAGTCTGTTC 
CCACCCTATT TGGAGATGGA GAGTATAAAG CAGGACAGTG CATTGGCACC CGACCCATTA 
AACCCCTACA GGCTGAGCTC AGTATCCAAG AAACACGGGA AACTGATCAC TTTTCTGCGC 
ACATTCATGA AGTCCAGGCC GACCAAGCAG AAGCTGAAGC AAAGAGGTAT CCTCAGGGAG 
AGGGTGTTCG GCTGTGACCT AGGAGAGCAT CTCCTCAACT CTGGCCATGA TGTCCCCCAG 
GTACTCAAGA GCTGCACCGA GTTCATTGAG AAGCATGGAG TGGTTGATGG AATGTATCGT 
CTCTCTGGGA TCGCCTCCAA CATTCAGAAG CTGCGGCACG AGTTTGATTC TGAACAGATC 
CCAGATCTGA CAAAAGATGT TTACATTCAG GATATACACT GCGTGGGCTC CCTGTGCAAG 
CTCTACTTTC GAGAGCTTCC CAACCCACTT CTCACCTACC AGCTGTACGA GAAATTCTCG 
GAGGCTGTGT CAGCAGCCAC AGACGAGGAG AGGCTGATCA AAATACATGA CGTCATTCAG 
CAGCTGCCGC CTCCGCATTA TAGAACCCTT GAGTTTCTTA TGAGGCATCT TTCACACTTG 
GCAACCTTCA GCTATGTAAC GAACATGCAT ACTAAAAATT TGGCCATCGT TTGGGCCCCC 
AACTTACTGA GGTCAAAGCA AATAGAATCT GCTTGCTTCA GTGGGACAGC AGCCTTCATG 
GAAGTACGAA TACAATCGGT TGTGGTGGAG TTCATCCTGA ATCATGTGGA TGTTCTCTTC 
AGTCCCAAAC TGAGCTCTCT TATTCGAGAG GGAACAGGAC ACAACTCTTT GTCTCGGCCT 
AAGTCTTTGC TAGTACCGTC TCCATCCACA AAGCTGCTAA CTCTAGAAGA GGCACAGGCA 
CGCACACAGG CCCAGATCAA CTCACCCATC ACTGCAGACA GCAAGTACAT AGAGGTCGGA 
GAGGGTCCTG CAGCATTACA AGGAAAATTC CACACAGTCA TAGAATTCCC TACTGAGAGG 
AAGAGGCAGC CCATGAAGTC TAAGAAGTCT CCAGTTGGCA GTTGGCGTTC TTTTTTCAAC 
CTGGGCAAGT CTTCTTCAAT GTCGAAACGC AAACTACAGC GTAATCCCAG TGAACCAAAT 
GAACTCAAGG CTATGGCACT GGCTGGAGGA AGAGGAGACA CTGGTACTCT TCGGTCAGCC 
AAAAGTGAAG AGTCTCTCAC CTCTCTACAT AATGTTGAAG GAGAGTCTAA AGTGTATAGG 
CCCCGCAGAC CTCGCTCCAG CAGTGATGCC CTCTCTGCTT CATTCAATGG TGACCTTCTG 
GATACCAGAC AGCACTGTAA CTCGTACGAC AACCTAGGTC ATGGAGACAG TGATGGGGAT 
GATGGTCCAA TCTGTGTGCC TGCTCTCATC TCCCCACCAC GCTCCGCTGA TGATGTGGAT 
CTGAGCCCCC CTGACATTGG CATGGCCTCT TTAGACTTTG ACCCCATGTC TTTCCAATGT 
AGCCTTCCTC TGGCAGAAAC TTCGATTTTC TCAATTGACG CAGATACAAG CAGTCTAAAA 
AGGAGCCCTG CCAGTGGCTC AGAGTCAGTC TCGCCAGTCC GAGCTAAAGT TACGAGCCGG 
TTTTTGTCTC CAGACCACAG TACAGCCTTA AAGGATAAAC ATAAACTGCT CCTTACATCT 
TTCTCTGAGA AAACTCCAGT TCAGTCTGTT GAAAGCACCG AGATGTCAGC AGTTGTTTCT 
CCTCCAAGCA TCTCTGAATC AGCTGCCTCC GTGATAATGA AAGGACCAGA GAGCACTGTG 
CTTCAGCCCT CTTCCCCTCC ATCTCCACTC GACTCTCCTA CAAAATTGAG CTCTCTGTCA 
AGAACCACCG ATCTGCCCCT GAGTGAAGCC ATTCAACAAG AGCTTCTTTC TAAAGCTGCT 
ACCTTTGAGA GTAAAGACAT ATCGAGCATT GAATGTTCAC AACCACCACA GGTCACCTGC 
TCTCAAGAAC AGCCAGGAGC CGTAGCTTTG GACTCTCCAA AGGGCAATGT CCCTGTCAGC 
TCTGTGTCCC TCGTCCCTCC CCCTCCCCCA CCAAAGAACG CTGCCCGCAT GCTTGCACTA 
GCTCTAGCTG AATCTGCCCA GCAGGCCACC ATTCTTTCTC AGAAGCAATC CTCTGGACCC 
CCTACACCAG TATCCCCTCT TAGGCCTCAA GAGTTATTGG AGACCCTGGA CTGGCCGCTG 
CCTTCTTCCT TGCCATCACA GCCATCCGAG GAGGAAACTG CAGTAGAATC ATCAAATAAA 
TCGGTTCCTT TGTCTTCACC ACCCTCCACT CCACCTGAAA AAATGACTCG CTCAGTCAGT 
CTGCACCTCA CCACAGCTGA TAGTGGGAAG CCATCCAGCA TTTCATGCAA CAGCCAGGAT 
GTAATCTCAC CAGAGACCCC AAGCCAGAGT GCTATGCCCT CTTGTCTGAC TTCTCATGTT 
TCTCAAACAC AAAAAAGTCC AGAAAAACAG CAGCCTGCTG TCGGTCAGAC ACCTGTGAGC 
ACAGCAAAAA CCACCACCAC AACCACCACT TCAACCATCA GCAGTAAGGA TGCTGTCATT 
CAACAACCCA CCTCTGAGAT CCATATGCCT GAAACAGTCC TGCCACCAAA ACCAAACAAG 
GTGCCAGATC AACCAGTGGC AATTCTTCAG CCTCAGCCTC AAATGCAGAC GCAACCTCAT 
ACTGCATTTC GGTCTCAACC TCAAGTTCAG CCTCTTCCTC AGTCACAAGC ACAGCCACAG 
TCTCAGCCCA GATCACAGCC CAATGCTAGA GTTGCACCAA CCCTGGCAGC ACCTCATGAT 
CCTGTGGAAA AACCATGGGA AGCCATAAAG CCTGTCCATC CAAAAGGAGA TAGTGCTTCT 
CAACAACACC ACACTCAGGG AGTGACACCA CCAACACCCC CTATTCGCTC GATTGAAAGT 
AAACTAGCAG CAGCTGCACT TTGCAACACC GAGGCTGCAA ACCCTGCCAA CTTTCATGCC 
ATGTTAGAGA CATCCATGAG GGGATCAGTA GAGGAGACTC CCCCACAGTC TCATCCGCCT 
CACCGCAGAT CGTCTACTCA TCAGTCAGGT TATCTTTATC ACACAAAAGG GGAGGCAGCC 
TTACTGGACC CCACCACAGA GGCATATTAC CATCAGCGGG TAACCCCTTC AGGTCAGGCT 
ACAGTGGGGT ACCATTACCG GCCAGAGAGT GTTCCACCTC ACATTTCTTA CGTGTCAAAA 
TCAGAGCCAC ACATTTCCTA CAGAGGTCAT GGGGATGGTC GATACAATAC AATTGGACCT 
AGATCCTACC ACCAGTCTAT CAAATCTCGT GGTTCACTTA GAAGTGAGTA CATCTCTCCA 
AGCTCATTAC ACCACTCCCA TAGCTACAGC CAAGCAGATG GACCAACCGT CTACCCAACA 
ATTCGCAGGG TCCACTCCTT ACATGTGCCT CCCACTGCTA TTCGCTCAAT GCCAGTCACC 
AGAACTGAGG TACCACCAGA TGATGAGTTA TTTTACTACC AGCGCCCAAT CTACCACTGC 
AAGTCTCACC AACCATCATC TCAAGTCGAC TATCACGTCA CACAACTGCA GCCTTATTTT 
GAAAATGGAA GAGTTCAGTA CCGCTACAGC CCCTACTCTG GCTCTCACGT TCTGGAATCC 
CCTTTCTATG ATGTGGACCA TTATGGTACT ATCCGCTTAA GGCACGTCCA TTCCTTCAGT 
GGTCGGGACA GTGCCCCTCT CCTTCGATCT GGAGCAAAAT CTGGAGGCTA CCACTACCTT 
TCACGGCATG TTATTCCTTT AAAGGAGCAT GGCTTCATAA GCAGGGACAT GCCACCTTAC 
AGTGGTTCAA AAGGGAGTAT TTACTTTGCA TGGGACCCAG AGGAGGCAGA AAGGCTGCGC 
ATACACTCCA TCCGTAGGGA AAGCAGAGCA AGGCAAAAGG TTAAGGGTTC TGTTATGTCT 
CAATACGACA ATGTAGGACC CTACATGCCT GTGGATGTTG ACATGTTACA TTTGCGCAGC 
AAATCAGATC CTGGAAAAAC TGTATTGGTG TCCTCGGAGA GCAAAGAGGC CAGATACAAT 
GTTGTGCCTG GATCTCAGCA TGCTCCCAGA CACTTGATCT CTGATCCAGA TGTCCTAATG 
TATATGGAAA CAGAGAAGCA CTGCCAAGGA AATGGAATGG GGGAGAAAAC CACCAAGCAA 
GGCAGCTCTA GGACCTACTC GTCTATACAT TCACATCAAT CCCAGCTTCC ACCACTAAGC 
CGGCAGCACT TACCAGAGGG TGGCCATCAT GACCCAAAGT TTGAACCAGG GGAAAAGCAA 
CTGAGTGGTA AACATTGGCA GGAACATTCG GACAGCAGGA GTGCTCAAGC ACGTTATGAA 
AGGTCTGATT TGGATCGCCA CATATGCAGG GTGAAGGCCA CTAGCAGTGC CAGTGAAGAT 
GAACAAGCTG CTCCTGTGAA GCCGGCTCCT CCACCTAAGC CCGAGAGATC CCATAGTGTT 
CGAGAGCGGC ACCATTACAA CCAGTCCAGT CTTCCTGCAT ATTTACCAGA CAATTCAGAT 
AGAGACCACA GTGGAACATA CTCCCATCAG TCACAGGGGC AGAGACAAAG CACCGTTACT 
TTGCAGTCGC ATTACGACAA CCTGGATGAC TATCATCCAG TACCTCAATC CCAAACCACG 
CTAGCAAATC GGGGTTGTTC CAGTTCCTAT CATAGCCCCG GTTTCTCAAC TCCACACAGT 
AATCGTGCAT ACTCCACTGC CTTGGGACAG GGTGCCTTCA TACAAGCTGA GCTTTCAGTG 
CAAAGGCCAG AGACTGAGAT AAATGCTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293688.1
CDS	524..6376
Translation	MEAGSGAAAVVGSAALGLLGATGSHDISDRGLRPGRHPEEDDIVPELARSIHPRERPDWEETISAMARSAEIPELSSEPLMRSCSSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGTIQLTLADEQTEVTRNGLESKEPVYLVQIYCQGRSWIVRRSYEDFRVLDKHLHLCIYDRRFSQLPELPRFDSLRERAEAVSQMLMAYLSRLSAIADNKINCGPALTWMEVDNKGNHLLVHEESSINVPAIAAAHVIKRYIAQAADELSFEVGDIVSVIDMPPKEDTTWWRGKHGFQVGFFPSECVELINDKVPQSVTNSVPKPASPCSGLRPASWSLFPPYLEMESIKQDSALAPDPLNPYRLSSVSKKHGKLITFLRTFMKSRPTKQKLKQRGILRERVFGCDLGEHLLNSGHDVPQVLKSCTEFIEKHGVVDGMYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSEAVSAATDEERLIKIHDVIQQLPPPHYRTLEFLMRHLSHLATFSYVTNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVDVLFSPKLSSLIREGTGHNSLSRPKSLLVPSPSTKLLTLEEAQARTQAQINSPITADSKYIEVGEGPAALQGKFHTVIEFPTERKRQPMKSKKSPVGSWRSFFNLGKSSSMSKRKLQRNPSEPNELKAMALAGGRGDTGTLRSAKSEESLTSLHNVEGESKVYRPRRPRSSSDALSASFNGDLLDTRQHCNSYDNLGHGDSDGDDGPICVPALISPPRSADDVDLSPPDIGMASLDFDPMSFQCSLPLAETSIFSIDADTSSLKRSPASGSESVSPVRAKVTSRFLSPDHSTALKDKHKLLLTSFSEKTPVQSVESTEMSAVVSPPSISESAASVIMKGPESTVLQPSSPPSPLDSPTKLSSLSRTTDLPLSEAIQQELLSKAATFESKDISSIECSQPPQVTCSQEQPGAVALDSPKGNVPVSSVSLVPPPPPPKNAARMLALALAESAQQATILSQKQSSGPPTPVSPLRPQELLETLDWPLPSSLPSQPSEEETAVESSNKSVPLSSPPSTPPEKMTRSVSLHLTTADSGKPSSISCNSQDVISPETPSQSAMPSCLTSHVSQTQKSPEKQQPAVGQTPVSTAKTTTTTTTSTISSKDAVIQQPTSEIHMPETVLPPKPNKVPDQPVAILQPQPQMQTQPHTAFRSQPQVQPLPQSQAQPQSQPRSQPNARVAPTLAAPHDPVEKPWEAIKPVHPKGDSASQQHHTQGVTPPTPPIRSIESKLAAAALCNTEAANPANFHAMLETSMRGSVEETPPQSHPPHRRSSTHQSGYLYHTKGEAALLDPTTEAYYHQRVTPSGQATVGYHYRPESVPPHISYVSKSEPHISYRGHGDGRYNTIGPRSYHQSIKSRGSLRSEYISPSSLHHSHSYSQADGPTVYPTIRRVHSLHVPPTAIRSMPVTRTEVPPDDELFYYQRPIYHCKSHQPSSQVDYHVTQLQPYFENGRVQYRYSPYSGSHVLESPFYDVDHYGTIRLRHVHSFSGRDSAPLLRSGAKSGGYHYLSRHVIPLKEHGFISRDMPPYSGSKGSIYFAWDPEEAERLRIHSIRRESRARQKVKGSVMSQYDNVGPYMPVDVDMLHLRSKSDPGKTVLVSSESKEARYNVVPGSQHAPRHLISDPDVLMYMETEKHCQGNGMGEKTTKQGSSRTYSSIHSHQSQLPPLSRQHLPEGGHHDPKFEPGEKQLSGKHWQEHSDSRSAQARYERSDLDRHICRVKATSSASEDEQAAPVKPAPPPKPERSHSVRERHHYNQSSLPAYLPDNSDRDHSGTYSHQSQGQRQSTVTLQSHYDNLDDYHPVPQSQTTLANRGCSSSYHSPGFSTPHSNRAYSTALGQGAFIQAELSVQRPETEINAE

Target ORF information:

RefSeq Version	XM_009295413.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295413.2

ORF Insert Sequence:

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGG GGCCTGAGGC CTGGACGGCA CCCTGAAGAA 
GACGATATCG TGCCAGAGCT TGCCAGGAGC ATCCACCCCC GAGAGCGACC TGACTGGGAG 
GAAACCATCA GCGCAATGGC ACGAAGTGCT GAAATTCCAG AGCTGAGCTC TGAGCCGCTC 
ATGCGCTCCT GCAGCAGCAC CGCCAGTATG AAGGTGAAGA ATGTCAAAAA GCTGTCATTC 
ACCAAGGGCC ATTTTCCAAA GTTAGCTGAA TGTGCCCACT TCCACTATGA AAACGTTGAT 
TTTGGAACAA TACAGTTAAC CCTCGCAGAT GAGCAGACTG AAGTGACACG GAATGGCCTG 
GAGTCAAAAG AGCCTGTGTA TTTGGTTCAG ATCTACTGTC AGGGACGAAG CTGGATCGTG 
AGACGAAGCT ATGAAGATTT CCGAGTCTTG GATAAGCATC TCCATCTCTG CATCTATGAC 
CGCCGATTTT CCCAGCTCCC TGAGCTGCCC CGTTTCGACA GCCTGAGGGA GAGAGCGGAG 
GCAGTTTCCC AGATGCTGAT GGCTTACCTC TCCCGTCTCT CAGCCATTGC TGATAACAAG 
ATCAATTGTG GGCCGGCCCT CACGTGGATG GAGGTTGACA ACAAAGGGAA TCATTTGCTG 
GTGCATGAAG AATCATCTAT AAATGTTCCA GCTATCGCAG CGGCCCACGT GATCAAGCGC 
TACATCGCAC AGGCAGCTGA TGAGCTGTCA TTTGAGGTGG GGGATATTGT TTCTGTCATT 
GACATGCCAC CCAAAGAGGA CACCACCTGG TGGAGGGGAA AACATGGTTT CCAGGTTGGC 
TTCTTTCCCA GCGAGTGTGT GGAGCTGATA AACGACAAGG TCCCGCAGTC TGTCACCAAC 
TCTGTGCCAA AACCAGCGTC CCCCTGCTCT GGTCTGCGGC CGGCATCCTG GAGTCTGTTC 
CCACCCTATT TGGAGATGGA GAGTATAAAG CAGGACAGTG CATTGGCACC CGACCCATTA 
AACCCCTACA GGCTGAGCTC AGTATCCAAG AAACACGGGA AACTGATCAC TTTTCTGCGC 
ACATTCATGA AGTCCAGGCC GACCAAGCAG AAGCTGAAGC AAAGAGGTAT CCTCAGGGAG 
AGGGTGTTCG GCTGTGACCT AGGAGAGCAT CTCCTCAACT CTGGCCATGA TGTCCCCCAG 
GTACTCAAGA GCTGCACCGA GTTCATTGAG AAGCATGGAG TGGTTGATGG AATGTATCGT 
CTCTCTGGGA TCGCCTCCAA CATTCAGAAG CTGCGGCACG AGTTTGATTC TGAACAGATC 
CCAGATCTGA CAAAAGATGT TTACATTCAG GATATACACT GCGTGGGCTC CCTGTGCAAG 
CTCTACTTTC GAGAGCTTCC CAACCCACTT CTCACCTACC AGCTGTACGA GAAATTCTCG 
GAGGCTGTGT CAGCAGCCAC AGACGAGGAG AGGCTGATCA AAATACATGA CGTCATTCAG 
CAGCTGCCGC CTCCGCATTA TAGAACCCTT GAGTTTCTTA TGAGGCATCT TTCACACTTG 
GCAACCTTCA GCTATGTAAC GAACATGCAT ACTAAAAATT TGGCCATCGT TTGGGCCCCC 
AACTTACTGA GGTCAAAGCA AATAGAATCT GCTTGCTTCA GTGGGACAGC AGCCTTCATG 
GAAGTACGAA TACAATCGGT TGTGGTGGAG TTCATCCTGA ATCATGTGGA TGTTCTCTTC 
AGTCCCAAAC TGAGCTCTCT TATTCGAGAG GGAACAGGAC ACAACTCTTT GTCTCGGCCT 
AAGTCTTTGC TAGTACCGTC TCCATCCACA AAGCTGCTAA CTCTAGAAGA GGCACAGGCA 
CGCACACAGG CCCAGATCAA CTCACCCATC ACTGCAGACA GCAAGTACAT AGAGGTCGGA 
GAGGGTCCTG CAGCATTACA AGGAAAATTC CACACAGTCA TAGAATTCCC TACTGAGAGG 
AAGAGGCAGC CCATGAAGTC TAAGAAGTCT CCAGTTGGCA GTTGGCGTTC TTTTTTCAAC 
CTGGGCAAGT CTTCTTCAAT GTCGAAACGC AAACTACAGC GTAATCCCAG TGAACCAAAT 
GAACTCAAGG CTATGGCACT GGCTGGAGGA AGAGGAGACA CTGGTACTCT TCGGTCAGCC 
AAAAGTGAAG AGTCTCTCAC CTCTCTACAT AATGTTGAAG GAGAGTCTAA AGTGTATAGG 
CCCCGCAGAC CTCGCTCCAG CAGTGATGCC CTCTCTGCTT CATTCAATGG TGACCTTCTG 
GATACCAGAC AGCACTGTAA CTCGTACGAC AACCTAGGTC ATGGAGACAG TGATGGGGAT 
GATGGTCCAA TCTGTGTGCC TGCTCTCATC TCCCCACCAC GCTCCGCTGA TGATGTGGAT 
CTGAGCCCCC CTGACATTGG CATGGCCTCT TTAGACTTTG ACCCCATGTC TTTCCAATGT 
AGCCTTCCTC TGGCAGAAAC TTCGATTTTC TCAATTGACG CAGATACAAG CAGTCTAAAA 
AGGAGCCCTG CCAGTGGCTC AGAGTCAGTC TCGCCAGTCC GAGCTAAAGT TACGAGCCGG 
TTTTTGTCTC CAGACCACAG TACAGCCTTA AAGGATAAAC ATAAACTGCT CCTTACATCT 
TTCTCTGAGA AAACTCCAGT TCAGTCTGTT GAAAGCACCG AGATGTCAGC AGTTGTTTCT 
CCTCCAAGCA TCTCTGAATC AGCTGCCTCC GTGATAATGA AAGGACCAGA GAGCACTGTG 
CTTCAGCCCT CTTCCCCTCC ATCTCCACTC GACTCTCCTA CAAAATTGAG CTCTCTGTCA 
AGAACCACCG ATCTGCCCCT GAGTGAAGCC ATTCAACAAG AGCTTCTTTC TAAAGCTGCT 
ACCTTTGAGA GTAAAGACAT ATCGAGCATT GAATGTTCAC AACCACCACA GGTCACCTGC 
TCTCAAGAAC AGCCAGGAGC CGTAGCTTTG GACTCTCCAA AGGGCAATGT CCCTGTCAGC 
TCTGTGTCCC TCGTCCCTCC CCCTCCCCCA CCAAAGAACG CTGCCCGCAT GCTTGCACTA 
GCTCTAGCTG AATCTGCCCA GCAGGCCACC ATTCTTTCTC AGAAGCAATC CTCTGGACCC 
CCTACACCAG TATCCCCTCT TAGGCCTCAA GAGTTATTGG AGACCCTGGA CTGGCCGCTG 
CCTTCTTCCT TGCCATCACA GCCATCCGAG GAGGAAACTG CAGTAGAATC ATCAAATAAA 
TCGGTTCCTT TGTCTTCACC ACCCTCCACT CCACCTGAAA AAATGACTCG CTCAGTCAGT 
CTGCACCTCA CCACAGCTGA TAGTGGGAAG CCATCCAGCA TTTCATGCAA CAGCCAGGAT 
GTAATCTCAC CAGAGACCCC AAGCCAGAGT GCTATGCCCT CTTGTCTGAC TTCTCATGTT 
TCTCAAACAC AAAAAAGTCC AGAAAAACAG CAGCCTGCTG TCGGTCAGAC ACCTGTGAGC 
ACAGCAAAAA CCACCACCAC AACCACCACT TCAACCATCA GCAGTAAGGA TGCTGTCATT 
CAACAACCCA CCTCTGAGAT CCATATGCCT GAAACAGTCC TGCCACCAAA ACCAAACAAG 
GTGCCAGATC AACCAGTGGC AATTCTTCAG CCTCAGCCTC AAATGCAGAC GCAACCTCAT 
ACTGCATTTC GGTCTCAACC TCAAGTTCAG CCTCTTCCTC AGTCACAAGC ACAGCCACAG 
TCTCAGCCCA GATCACAGCC CAATGCTAGA GTTGCACCAA CCCTGGCAGC ACCTCATGAT 
CCTGTGGAAA AACCATGGGA AGCCATAAAG CCTGTCCATC CAAAAGGAGA TAGTGCTTCT 
CAACAACACC ACACTCAGGG AGTGACACCA CCAACACCCC CTATTCGCTC GATTGAAAGT 
AAACTAGCAG CAGCTGCACT TTGCAACACC GAGGCTGCAA ACCCTGCCAA CTTTCATGCC 
ATGTTAGAGA CATCCATGAG GGGATCAGTA GAGGAGACTC CCCCACAGTC TCATCCGCCT 
CACCGCAGAT CGTCTACTCA TCAGTCAGGT TATCTTTATC ACACAAAAGG GGAGGCAGCC 
TTACTGGACC CCACCACAGA GGCATATTAC CATCAGCGGG TAACCCCTTC AGGTCAGGCT 
ACAGTGGGGT ACCATTACCG GCCAGAGAGT GTTCCACCTC ACATTTCTTA CGTGTCAAAA 
TCAGAGCCAC ACATTTCCTA CAGAGGTCAT GGGGATGGTC GATACAATAC AATTGGACCT 
AGATCCTACC ACCAGTCTAT CAAATCTCGT GGTTCACTTA GAAGTGAGTA CATCTCTCCA 
AGCTCATTAC ACCACTCCCA TAGCTACAGC CAAGCAGATG GACCAACCGT CTACCCAACA 
ATTCGCAGGG TCCACTCCTT ACATGTGCCT CCCACTGCTA TTCGCTCAAT GCCAGTCACC 
AGAACTGAGG TACCACCAGA TGATGAGTTA TTTTACTACC AGCGCCCAAT CTACCACTGC 
AAGTCTCACC AACCATCATC TCAAGTCGAC TATCACGTCA CACAACTGCA GCCTTATTTT 
GAAAATGGAA GAGTTCAGTA CCGCTACAGC CCCTACTCTG GCTCTCACGT TCTGGAATCC 
CCTTTCTATG ATGTGGACCA TTATGGTACT ATCCGCTTAA GGCACGTCCA TTCCTTCAGT 
GGTCGGGACA GTGCCCCTCT CCTTCGATCT GGAGCAAAAT CTGGAGGCTA CCACTACCTT 
TCACGGCATG TTATTCCTTT AAAGGAGCAT GGCTTCATAA GCAGGGACAT GCCACCTTAC 
AGTGGTTCAA AAGGGAGTAT TTACTTTGCA TGGGACCCAG AGGAGGCAGA AAGGCTGCGC 
ATACACTCCA TCCGTAGGGA AAGCAGAGCA AGGCAAAAGG TTAAGGGTTC TGTTATGTCT 
CAATACGACA ATGTAGGACC CTACATGCCT GTGGATGTTG ACATGTTACA TTTGCGCAGC 
AAATCAGATC CTGGAAAAAC TGTATTGGTG TCCTCGGAGA GCAAAGAGGC CAGATACAAT 
GTTGTGCCTG GATCTCAGCA TGCTCCCAGA CACTTGATCT CTGATCCAGA TGTCCTAATG 
TATATGGAAA CAGAGAAGCA CTGCCAAGGA AATGGAATGG GGGAGAAAAC CACCAAGCAA 
GGCAGCTCTA GGACCTACTC GTCTATACAT TCACATCAAT CCCAGCTTCC ACCACTAAGC 
CGGCAGCACT TACCAGAGGG TGGCCATCAT GACCCAAAGT TTGAACCAGG GGAAAAGCAA 
CTGAGTGGTA AACATTGGCA GGAACATTCG GACAGCAGGA GTGCTCAAGC ACGTTATGAA 
AGGTCTGATT TGGATCGCCA CATATGCAGG GTGAAGGCCA CTAGCAGTGC CAGTGAAGAT 
GAACAAGCTG CTCCTGTGAA GCCGGCTCCT CCACCTAAGC CCGAGAGATC CCATAGTGTT 
CGAGAGCGGC ACCATTACAA CCAGTCCAGT CTTCCTGCAT ATTTACCAGA CAATTCAGAT 
AGAGACCACA GTGGAACATA CTCCCATCAG TCACAGGGGC AGAGACAAAG CACCGTTACT 
TTGCAGTCGC ATTACGACAA CCTGGATGAC TATCATCCAG TACCTCAATC CCAAACCACG 
CTAGCAAATC GGGGTTGTTC CAGTTCCTAT CATAGCCCCG GTTTCTCAAC TCCACACAGT 
AATCGTGCAT ACTCCACTGC CTTGGGACAG GGTGCCTTCA TACAAGCTGA GCTTTCAGTG 
CAAAGGCCAG AGACTGAGAT AAATGCTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32b ( XM_009295413.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293688.1 Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X4, mRNA.

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CloneID	ODa45740
Clone ID Related Accession (Same CDS sequence)	XM_021469079.1 , XM_009295413.2 , XM_009295413.3
Accession Version	XM_009295413.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5853bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho GTPase-activating protein 32 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295413.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGG GGCCTGAGGC CTGGACGGCA CCCTGAAGAA 
GACGATATCG TGCCAGAGCT TGCCAGGAGC ATCCACCCCC GAGAGCGACC TGACTGGGAG 
GAAACCATCA GCGCAATGGC ACGAAGTGCT GAAATTCCAG AGCTGAGCTC TGAGCCGCTC 
ATGCGCTCCT GCAGCAGCAC CGCCAGTATG AAGGTGAAGA ATGTCAAAAA GCTGTCATTC 
ACCAAGGGCC ATTTTCCAAA GTTAGCTGAA TGTGCCCACT TCCACTATGA AAACGTTGAT 
TTTGGAACAA TACAGTTAAC CCTCGCAGAT GAGCAGACTG AAGTGACACG GAATGGCCTG 
GAGTCAAAAG AGCCTGTGTA TTTGGTTCAG ATCTACTGTC AGGGACGAAG CTGGATCGTG 
AGACGAAGCT ATGAAGATTT CCGAGTCTTG GATAAGCATC TCCATCTCTG CATCTATGAC 
CGCCGATTTT CCCAGCTCCC TGAGCTGCCC CGTTTCGACA GCCTGAGGGA GAGAGCGGAG 
GCAGTTTCCC AGATGCTGAT GGCTTACCTC TCCCGTCTCT CAGCCATTGC TGATAACAAG 
ATCAATTGTG GGCCGGCCCT CACGTGGATG GAGGTTGACA ACAAAGGGAA TCATTTGCTG 
GTGCATGAAG AATCATCTAT AAATGTTCCA GCTATCGCAG CGGCCCACGT GATCAAGCGC 
TACATCGCAC AGGCAGCTGA TGAGCTGTCA TTTGAGGTGG GGGATATTGT TTCTGTCATT 
GACATGCCAC CCAAAGAGGA CACCACCTGG TGGAGGGGAA AACATGGTTT CCAGGTTGGC 
TTCTTTCCCA GCGAGTGTGT GGAGCTGATA AACGACAAGG TCCCGCAGTC TGTCACCAAC 
TCTGTGCCAA AACCAGCGTC CCCCTGCTCT GGTCTGCGGC CGGCATCCTG GAGTCTGTTC 
CCACCCTATT TGGAGATGGA GAGTATAAAG CAGGACAGTG CATTGGCACC CGACCCATTA 
AACCCCTACA GGCTGAGCTC AGTATCCAAG AAACACGGGA AACTGATCAC TTTTCTGCGC 
ACATTCATGA AGTCCAGGCC GACCAAGCAG AAGCTGAAGC AAAGAGGTAT CCTCAGGGAG 
AGGGTGTTCG GCTGTGACCT AGGAGAGCAT CTCCTCAACT CTGGCCATGA TGTCCCCCAG 
GTACTCAAGA GCTGCACCGA GTTCATTGAG AAGCATGGAG TGGTTGATGG AATGTATCGT 
CTCTCTGGGA TCGCCTCCAA CATTCAGAAG CTGCGGCACG AGTTTGATTC TGAACAGATC 
CCAGATCTGA CAAAAGATGT TTACATTCAG GATATACACT GCGTGGGCTC CCTGTGCAAG 
CTCTACTTTC GAGAGCTTCC CAACCCACTT CTCACCTACC AGCTGTACGA GAAATTCTCG 
GAGGCTGTGT CAGCAGCCAC AGACGAGGAG AGGCTGATCA AAATACATGA CGTCATTCAG 
CAGCTGCCGC CTCCGCATTA TAGAACCCTT GAGTTTCTTA TGAGGCATCT TTCACACTTG 
GCAACCTTCA GCTATGTAAC GAACATGCAT ACTAAAAATT TGGCCATCGT TTGGGCCCCC 
AACTTACTGA GGTCAAAGCA AATAGAATCT GCTTGCTTCA GTGGGACAGC AGCCTTCATG 
GAAGTACGAA TACAATCGGT TGTGGTGGAG TTCATCCTGA ATCATGTGGA TGTTCTCTTC 
AGTCCCAAAC TGAGCTCTCT TATTCGAGAG GGAACAGGAC ACAACTCTTT GTCTCGGCCT 
AAGTCTTTGC TAGTACCGTC TCCATCCACA AAGCTGCTAA CTCTAGAAGA GGCACAGGCA 
CGCACACAGG CCCAGATCAA CTCACCCATC ACTGCAGACA GCAAGTACAT AGAGGTCGGA 
GAGGGTCCTG CAGCATTACA AGGAAAATTC CACACAGTCA TAGAATTCCC TACTGAGAGG 
AAGAGGCAGC CCATGAAGTC TAAGAAGTCT CCAGTTGGCA GTTGGCGTTC TTTTTTCAAC 
CTGGGCAAGT CTTCTTCAAT GTCGAAACGC AAACTACAGC GTAATCCCAG TGAACCAAAT 
GAACTCAAGG CTATGGCACT GGCTGGAGGA AGAGGAGACA CTGGTACTCT TCGGTCAGCC 
AAAAGTGAAG AGTCTCTCAC CTCTCTACAT AATGTTGAAG GAGAGTCTAA AGTGTATAGG 
CCCCGCAGAC CTCGCTCCAG CAGTGATGCC CTCTCTGCTT CATTCAATGG TGACCTTCTG 
GATACCAGAC AGCACTGTAA CTCGTACGAC AACCTAGGTC ATGGAGACAG TGATGGGGAT 
GATGGTCCAA TCTGTGTGCC TGCTCTCATC TCCCCACCAC GCTCCGCTGA TGATGTGGAT 
CTGAGCCCCC CTGACATTGG CATGGCCTCT TTAGACTTTG ACCCCATGTC TTTCCAATGT 
AGCCTTCCTC TGGCAGAAAC TTCGATTTTC TCAATTGACG CAGATACAAG CAGTCTAAAA 
AGGAGCCCTG CCAGTGGCTC AGAGTCAGTC TCGCCAGTCC GAGCTAAAGT TACGAGCCGG 
TTTTTGTCTC CAGACCACAG TACAGCCTTA AAGGATAAAC ATAAACTGCT CCTTACATCT 
TTCTCTGAGA AAACTCCAGT TCAGTCTGTT GAAAGCACCG AGATGTCAGC AGTTGTTTCT 
CCTCCAAGCA TCTCTGAATC AGCTGCCTCC GTGATAATGA AAGGACCAGA GAGCACTGTG 
CTTCAGCCCT CTTCCCCTCC ATCTCCACTC GACTCTCCTA CAAAATTGAG CTCTCTGTCA 
AGAACCACCG ATCTGCCCCT GAGTGAAGCC ATTCAACAAG AGCTTCTTTC TAAAGCTGCT 
ACCTTTGAGA GTAAAGACAT ATCGAGCATT GAATGTTCAC AACCACCACA GGTCACCTGC 
TCTCAAGAAC AGCCAGGAGC CGTAGCTTTG GACTCTCCAA AGGGCAATGT CCCTGTCAGC 
TCTGTGTCCC TCGTCCCTCC CCCTCCCCCA CCAAAGAACG CTGCCCGCAT GCTTGCACTA 
GCTCTAGCTG AATCTGCCCA GCAGGCCACC ATTCTTTCTC AGAAGCAATC CTCTGGACCC 
CCTACACCAG TATCCCCTCT TAGGCCTCAA GAGTTATTGG AGACCCTGGA CTGGCCGCTG 
CCTTCTTCCT TGCCATCACA GCCATCCGAG GAGGAAACTG CAGTAGAATC ATCAAATAAA 
TCGGTTCCTT TGTCTTCACC ACCCTCCACT CCACCTGAAA AAATGACTCG CTCAGTCAGT 
CTGCACCTCA CCACAGCTGA TAGTGGGAAG CCATCCAGCA TTTCATGCAA CAGCCAGGAT 
GTAATCTCAC CAGAGACCCC AAGCCAGAGT GCTATGCCCT CTTGTCTGAC TTCTCATGTT 
TCTCAAACAC AAAAAAGTCC AGAAAAACAG CAGCCTGCTG TCGGTCAGAC ACCTGTGAGC 
ACAGCAAAAA CCACCACCAC AACCACCACT TCAACCATCA GCAGTAAGGA TGCTGTCATT 
CAACAACCCA CCTCTGAGAT CCATATGCCT GAAACAGTCC TGCCACCAAA ACCAAACAAG 
GTGCCAGATC AACCAGTGGC AATTCTTCAG CCTCAGCCTC AAATGCAGAC GCAACCTCAT 
ACTGCATTTC GGTCTCAACC TCAAGTTCAG CCTCTTCCTC AGTCACAAGC ACAGCCACAG 
TCTCAGCCCA GATCACAGCC CAATGCTAGA GTTGCACCAA CCCTGGCAGC ACCTCATGAT 
CCTGTGGAAA AACCATGGGA AGCCATAAAG CCTGTCCATC CAAAAGGAGA TAGTGCTTCT 
CAACAACACC ACACTCAGGG AGTGACACCA CCAACACCCC CTATTCGCTC GATTGAAAGT 
AAACTAGCAG CAGCTGCACT TTGCAACACC GAGGCTGCAA ACCCTGCCAA CTTTCATGCC 
ATGTTAGAGA CATCCATGAG GGGATCAGTA GAGGAGACTC CCCCACAGTC TCATCCGCCT 
CACCGCAGAT CGTCTACTCA TCAGTCAGGT TATCTTTATC ACACAAAAGG GGAGGCAGCC 
TTACTGGACC CCACCACAGA GGCATATTAC CATCAGCGGG TAACCCCTTC AGGTCAGGCT 
ACAGTGGGGT ACCATTACCG GCCAGAGAGT GTTCCACCTC ACATTTCTTA CGTGTCAAAA 
TCAGAGCCAC ACATTTCCTA CAGAGGTCAT GGGGATGGTC GATACAATAC AATTGGACCT 
AGATCCTACC ACCAGTCTAT CAAATCTCGT GGTTCACTTA GAAGTGAGTA CATCTCTCCA 
AGCTCATTAC ACCACTCCCA TAGCTACAGC CAAGCAGATG GACCAACCGT CTACCCAACA 
ATTCGCAGGG TCCACTCCTT ACATGTGCCT CCCACTGCTA TTCGCTCAAT GCCAGTCACC 
AGAACTGAGG TACCACCAGA TGATGAGTTA TTTTACTACC AGCGCCCAAT CTACCACTGC 
AAGTCTCACC AACCATCATC TCAAGTCGAC TATCACGTCA CACAACTGCA GCCTTATTTT 
GAAAATGGAA GAGTTCAGTA CCGCTACAGC CCCTACTCTG GCTCTCACGT TCTGGAATCC 
CCTTTCTATG ATGTGGACCA TTATGGTACT ATCCGCTTAA GGCACGTCCA TTCCTTCAGT 
GGTCGGGACA GTGCCCCTCT CCTTCGATCT GGAGCAAAAT CTGGAGGCTA CCACTACCTT 
TCACGGCATG TTATTCCTTT AAAGGAGCAT GGCTTCATAA GCAGGGACAT GCCACCTTAC 
AGTGGTTCAA AAGGGAGTAT TTACTTTGCA TGGGACCCAG AGGAGGCAGA AAGGCTGCGC 
ATACACTCCA TCCGTAGGGA AAGCAGAGCA AGGCAAAAGG TTAAGGGTTC TGTTATGTCT 
CAATACGACA ATGTAGGACC CTACATGCCT GTGGATGTTG ACATGTTACA TTTGCGCAGC 
AAATCAGATC CTGGAAAAAC TGTATTGGTG TCCTCGGAGA GCAAAGAGGC CAGATACAAT 
GTTGTGCCTG GATCTCAGCA TGCTCCCAGA CACTTGATCT CTGATCCAGA TGTCCTAATG 
TATATGGAAA CAGAGAAGCA CTGCCAAGGA AATGGAATGG GGGAGAAAAC CACCAAGCAA 
GGCAGCTCTA GGACCTACTC GTCTATACAT TCACATCAAT CCCAGCTTCC ACCACTAAGC 
CGGCAGCACT TACCAGAGGG TGGCCATCAT GACCCAAAGT TTGAACCAGG GGAAAAGCAA 
CTGAGTGGTA AACATTGGCA GGAACATTCG GACAGCAGGA GTGCTCAAGC ACGTTATGAA 
AGGTCTGATT TGGATCGCCA CATATGCAGG GTGAAGGCCA CTAGCAGTGC CAGTGAAGAT 
GAACAAGCTG CTCCTGTGAA GCCGGCTCCT CCACCTAAGC CCGAGAGATC CCATAGTGTT 
CGAGAGCGGC ACCATTACAA CCAGTCCAGT CTTCCTGCAT ATTTACCAGA CAATTCAGAT 
AGAGACCACA GTGGAACATA CTCCCATCAG TCACAGGGGC AGAGACAAAG CACCGTTACT 
TTGCAGTCGC ATTACGACAA CCTGGATGAC TATCATCCAG TACCTCAATC CCAAACCACG 
CTAGCAAATC GGGGTTGTTC CAGTTCCTAT CATAGCCCCG GTTTCTCAAC TCCACACAGT 
AATCGTGCAT ACTCCACTGC CTTGGGACAG GGTGCCTTCA TACAAGCTGA GCTTTCAGTG 
CAAAGGCCAG AGACTGAGAT AAATGCTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293688.1
CDS	521..6373
Translation	MEAGSGAAAVVGSAALGLLGATGSHDISDRGLRPGRHPEEDDIVPELARSIHPRERPDWEETISAMARSAEIPELSSEPLMRSCSSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGTIQLTLADEQTEVTRNGLESKEPVYLVQIYCQGRSWIVRRSYEDFRVLDKHLHLCIYDRRFSQLPELPRFDSLRERAEAVSQMLMAYLSRLSAIADNKINCGPALTWMEVDNKGNHLLVHEESSINVPAIAAAHVIKRYIAQAADELSFEVGDIVSVIDMPPKEDTTWWRGKHGFQVGFFPSECVELINDKVPQSVTNSVPKPASPCSGLRPASWSLFPPYLEMESIKQDSALAPDPLNPYRLSSVSKKHGKLITFLRTFMKSRPTKQKLKQRGILRERVFGCDLGEHLLNSGHDVPQVLKSCTEFIEKHGVVDGMYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSEAVSAATDEERLIKIHDVIQQLPPPHYRTLEFLMRHLSHLATFSYVTNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVDVLFSPKLSSLIREGTGHNSLSRPKSLLVPSPSTKLLTLEEAQARTQAQINSPITADSKYIEVGEGPAALQGKFHTVIEFPTERKRQPMKSKKSPVGSWRSFFNLGKSSSMSKRKLQRNPSEPNELKAMALAGGRGDTGTLRSAKSEESLTSLHNVEGESKVYRPRRPRSSSDALSASFNGDLLDTRQHCNSYDNLGHGDSDGDDGPICVPALISPPRSADDVDLSPPDIGMASLDFDPMSFQCSLPLAETSIFSIDADTSSLKRSPASGSESVSPVRAKVTSRFLSPDHSTALKDKHKLLLTSFSEKTPVQSVESTEMSAVVSPPSISESAASVIMKGPESTVLQPSSPPSPLDSPTKLSSLSRTTDLPLSEAIQQELLSKAATFESKDISSIECSQPPQVTCSQEQPGAVALDSPKGNVPVSSVSLVPPPPPPKNAARMLALALAESAQQATILSQKQSSGPPTPVSPLRPQELLETLDWPLPSSLPSQPSEEETAVESSNKSVPLSSPPSTPPEKMTRSVSLHLTTADSGKPSSISCNSQDVISPETPSQSAMPSCLTSHVSQTQKSPEKQQPAVGQTPVSTAKTTTTTTTSTISSKDAVIQQPTSEIHMPETVLPPKPNKVPDQPVAILQPQPQMQTQPHTAFRSQPQVQPLPQSQAQPQSQPRSQPNARVAPTLAAPHDPVEKPWEAIKPVHPKGDSASQQHHTQGVTPPTPPIRSIESKLAAAALCNTEAANPANFHAMLETSMRGSVEETPPQSHPPHRRSSTHQSGYLYHTKGEAALLDPTTEAYYHQRVTPSGQATVGYHYRPESVPPHISYVSKSEPHISYRGHGDGRYNTIGPRSYHQSIKSRGSLRSEYISPSSLHHSHSYSQADGPTVYPTIRRVHSLHVPPTAIRSMPVTRTEVPPDDELFYYQRPIYHCKSHQPSSQVDYHVTQLQPYFENGRVQYRYSPYSGSHVLESPFYDVDHYGTIRLRHVHSFSGRDSAPLLRSGAKSGGYHYLSRHVIPLKEHGFISRDMPPYSGSKGSIYFAWDPEEAERLRIHSIRRESRARQKVKGSVMSQYDNVGPYMPVDVDMLHLRSKSDPGKTVLVSSESKEARYNVVPGSQHAPRHLISDPDVLMYMETEKHCQGNGMGEKTTKQGSSRTYSSIHSHQSQLPPLSRQHLPEGGHHDPKFEPGEKQLSGKHWQEHSDSRSAQARYERSDLDRHICRVKATSSASEDEQAAPVKPAPPPKPERSHSVRERHHYNQSSLPAYLPDNSDRDHSGTYSHQSQGQRQSTVTLQSHYDNLDDYHPVPQSQTTLANRGCSSSYHSPGFSTPHSNRAYSTALGQGAFIQAELSVQRPETEINAE

Target ORF information:

RefSeq Version	XM_009295413.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295413.3

ORF Insert Sequence:

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGG GGCCTGAGGC CTGGACGGCA CCCTGAAGAA 
GACGATATCG TGCCAGAGCT TGCCAGGAGC ATCCACCCCC GAGAGCGACC TGACTGGGAG 
GAAACCATCA GCGCAATGGC ACGAAGTGCT GAAATTCCAG AGCTGAGCTC TGAGCCGCTC 
ATGCGCTCCT GCAGCAGCAC CGCCAGTATG AAGGTGAAGA ATGTCAAAAA GCTGTCATTC 
ACCAAGGGCC ATTTTCCAAA GTTAGCTGAA TGTGCCCACT TCCACTATGA AAACGTTGAT 
TTTGGAACAA TACAGTTAAC CCTCGCAGAT GAGCAGACTG AAGTGACACG GAATGGCCTG 
GAGTCAAAAG AGCCTGTGTA TTTGGTTCAG ATCTACTGTC AGGGACGAAG CTGGATCGTG 
AGACGAAGCT ATGAAGATTT CCGAGTCTTG GATAAGCATC TCCATCTCTG CATCTATGAC 
CGCCGATTTT CCCAGCTCCC TGAGCTGCCC CGTTTCGACA GCCTGAGGGA GAGAGCGGAG 
GCAGTTTCCC AGATGCTGAT GGCTTACCTC TCCCGTCTCT CAGCCATTGC TGATAACAAG 
ATCAATTGTG GGCCGGCCCT CACGTGGATG GAGGTTGACA ACAAAGGGAA TCATTTGCTG 
GTGCATGAAG AATCATCTAT AAATGTTCCA GCTATCGCAG CGGCCCACGT GATCAAGCGC 
TACATCGCAC AGGCAGCTGA TGAGCTGTCA TTTGAGGTGG GGGATATTGT TTCTGTCATT 
GACATGCCAC CCAAAGAGGA CACCACCTGG TGGAGGGGAA AACATGGTTT CCAGGTTGGC 
TTCTTTCCCA GCGAGTGTGT GGAGCTGATA AACGACAAGG TCCCGCAGTC TGTCACCAAC 
TCTGTGCCAA AACCAGCGTC CCCCTGCTCT GGTCTGCGGC CGGCATCCTG GAGTCTGTTC 
CCACCCTATT TGGAGATGGA GAGTATAAAG CAGGACAGTG CATTGGCACC CGACCCATTA 
AACCCCTACA GGCTGAGCTC AGTATCCAAG AAACACGGGA AACTGATCAC TTTTCTGCGC 
ACATTCATGA AGTCCAGGCC GACCAAGCAG AAGCTGAAGC AAAGAGGTAT CCTCAGGGAG 
AGGGTGTTCG GCTGTGACCT AGGAGAGCAT CTCCTCAACT CTGGCCATGA TGTCCCCCAG 
GTACTCAAGA GCTGCACCGA GTTCATTGAG AAGCATGGAG TGGTTGATGG AATGTATCGT 
CTCTCTGGGA TCGCCTCCAA CATTCAGAAG CTGCGGCACG AGTTTGATTC TGAACAGATC 
CCAGATCTGA CAAAAGATGT TTACATTCAG GATATACACT GCGTGGGCTC CCTGTGCAAG 
CTCTACTTTC GAGAGCTTCC CAACCCACTT CTCACCTACC AGCTGTACGA GAAATTCTCG 
GAGGCTGTGT CAGCAGCCAC AGACGAGGAG AGGCTGATCA AAATACATGA CGTCATTCAG 
CAGCTGCCGC CTCCGCATTA TAGAACCCTT GAGTTTCTTA TGAGGCATCT TTCACACTTG 
GCAACCTTCA GCTATGTAAC GAACATGCAT ACTAAAAATT TGGCCATCGT TTGGGCCCCC 
AACTTACTGA GGTCAAAGCA AATAGAATCT GCTTGCTTCA GTGGGACAGC AGCCTTCATG 
GAAGTACGAA TACAATCGGT TGTGGTGGAG TTCATCCTGA ATCATGTGGA TGTTCTCTTC 
AGTCCCAAAC TGAGCTCTCT TATTCGAGAG GGAACAGGAC ACAACTCTTT GTCTCGGCCT 
AAGTCTTTGC TAGTACCGTC TCCATCCACA AAGCTGCTAA CTCTAGAAGA GGCACAGGCA 
CGCACACAGG CCCAGATCAA CTCACCCATC ACTGCAGACA GCAAGTACAT AGAGGTCGGA 
GAGGGTCCTG CAGCATTACA AGGAAAATTC CACACAGTCA TAGAATTCCC TACTGAGAGG 
AAGAGGCAGC CCATGAAGTC TAAGAAGTCT CCAGTTGGCA GTTGGCGTTC TTTTTTCAAC 
CTGGGCAAGT CTTCTTCAAT GTCGAAACGC AAACTACAGC GTAATCCCAG TGAACCAAAT 
GAACTCAAGG CTATGGCACT GGCTGGAGGA AGAGGAGACA CTGGTACTCT TCGGTCAGCC 
AAAAGTGAAG AGTCTCTCAC CTCTCTACAT AATGTTGAAG GAGAGTCTAA AGTGTATAGG 
CCCCGCAGAC CTCGCTCCAG CAGTGATGCC CTCTCTGCTT CATTCAATGG TGACCTTCTG 
GATACCAGAC AGCACTGTAA CTCGTACGAC AACCTAGGTC ATGGAGACAG TGATGGGGAT 
GATGGTCCAA TCTGTGTGCC TGCTCTCATC TCCCCACCAC GCTCCGCTGA TGATGTGGAT 
CTGAGCCCCC CTGACATTGG CATGGCCTCT TTAGACTTTG ACCCCATGTC TTTCCAATGT 
AGCCTTCCTC TGGCAGAAAC TTCGATTTTC TCAATTGACG CAGATACAAG CAGTCTAAAA 
AGGAGCCCTG CCAGTGGCTC AGAGTCAGTC TCGCCAGTCC GAGCTAAAGT TACGAGCCGG 
TTTTTGTCTC CAGACCACAG TACAGCCTTA AAGGATAAAC ATAAACTGCT CCTTACATCT 
TTCTCTGAGA AAACTCCAGT TCAGTCTGTT GAAAGCACCG AGATGTCAGC AGTTGTTTCT 
CCTCCAAGCA TCTCTGAATC AGCTGCCTCC GTGATAATGA AAGGACCAGA GAGCACTGTG 
CTTCAGCCCT CTTCCCCTCC ATCTCCACTC GACTCTCCTA CAAAATTGAG CTCTCTGTCA 
AGAACCACCG ATCTGCCCCT GAGTGAAGCC ATTCAACAAG AGCTTCTTTC TAAAGCTGCT 
ACCTTTGAGA GTAAAGACAT ATCGAGCATT GAATGTTCAC AACCACCACA GGTCACCTGC 
TCTCAAGAAC AGCCAGGAGC CGTAGCTTTG GACTCTCCAA AGGGCAATGT CCCTGTCAGC 
TCTGTGTCCC TCGTCCCTCC CCCTCCCCCA CCAAAGAACG CTGCCCGCAT GCTTGCACTA 
GCTCTAGCTG AATCTGCCCA GCAGGCCACC ATTCTTTCTC AGAAGCAATC CTCTGGACCC 
CCTACACCAG TATCCCCTCT TAGGCCTCAA GAGTTATTGG AGACCCTGGA CTGGCCGCTG 
CCTTCTTCCT TGCCATCACA GCCATCCGAG GAGGAAACTG CAGTAGAATC ATCAAATAAA 
TCGGTTCCTT TGTCTTCACC ACCCTCCACT CCACCTGAAA AAATGACTCG CTCAGTCAGT 
CTGCACCTCA CCACAGCTGA TAGTGGGAAG CCATCCAGCA TTTCATGCAA CAGCCAGGAT 
GTAATCTCAC CAGAGACCCC AAGCCAGAGT GCTATGCCCT CTTGTCTGAC TTCTCATGTT 
TCTCAAACAC AAAAAAGTCC AGAAAAACAG CAGCCTGCTG TCGGTCAGAC ACCTGTGAGC 
ACAGCAAAAA CCACCACCAC AACCACCACT TCAACCATCA GCAGTAAGGA TGCTGTCATT 
CAACAACCCA CCTCTGAGAT CCATATGCCT GAAACAGTCC TGCCACCAAA ACCAAACAAG 
GTGCCAGATC AACCAGTGGC AATTCTTCAG CCTCAGCCTC AAATGCAGAC GCAACCTCAT 
ACTGCATTTC GGTCTCAACC TCAAGTTCAG CCTCTTCCTC AGTCACAAGC ACAGCCACAG 
TCTCAGCCCA GATCACAGCC CAATGCTAGA GTTGCACCAA CCCTGGCAGC ACCTCATGAT 
CCTGTGGAAA AACCATGGGA AGCCATAAAG CCTGTCCATC CAAAAGGAGA TAGTGCTTCT 
CAACAACACC ACACTCAGGG AGTGACACCA CCAACACCCC CTATTCGCTC GATTGAAAGT 
AAACTAGCAG CAGCTGCACT TTGCAACACC GAGGCTGCAA ACCCTGCCAA CTTTCATGCC 
ATGTTAGAGA CATCCATGAG GGGATCAGTA GAGGAGACTC CCCCACAGTC TCATCCGCCT 
CACCGCAGAT CGTCTACTCA TCAGTCAGGT TATCTTTATC ACACAAAAGG GGAGGCAGCC 
TTACTGGACC CCACCACAGA GGCATATTAC CATCAGCGGG TAACCCCTTC AGGTCAGGCT 
ACAGTGGGGT ACCATTACCG GCCAGAGAGT GTTCCACCTC ACATTTCTTA CGTGTCAAAA 
TCAGAGCCAC ACATTTCCTA CAGAGGTCAT GGGGATGGTC GATACAATAC AATTGGACCT 
AGATCCTACC ACCAGTCTAT CAAATCTCGT GGTTCACTTA GAAGTGAGTA CATCTCTCCA 
AGCTCATTAC ACCACTCCCA TAGCTACAGC CAAGCAGATG GACCAACCGT CTACCCAACA 
ATTCGCAGGG TCCACTCCTT ACATGTGCCT CCCACTGCTA TTCGCTCAAT GCCAGTCACC 
AGAACTGAGG TACCACCAGA TGATGAGTTA TTTTACTACC AGCGCCCAAT CTACCACTGC 
AAGTCTCACC AACCATCATC TCAAGTCGAC TATCACGTCA CACAACTGCA GCCTTATTTT 
GAAAATGGAA GAGTTCAGTA CCGCTACAGC CCCTACTCTG GCTCTCACGT TCTGGAATCC 
CCTTTCTATG ATGTGGACCA TTATGGTACT ATCCGCTTAA GGCACGTCCA TTCCTTCAGT 
GGTCGGGACA GTGCCCCTCT CCTTCGATCT GGAGCAAAAT CTGGAGGCTA CCACTACCTT 
TCACGGCATG TTATTCCTTT AAAGGAGCAT GGCTTCATAA GCAGGGACAT GCCACCTTAC 
AGTGGTTCAA AAGGGAGTAT TTACTTTGCA TGGGACCCAG AGGAGGCAGA AAGGCTGCGC 
ATACACTCCA TCCGTAGGGA AAGCAGAGCA AGGCAAAAGG TTAAGGGTTC TGTTATGTCT 
CAATACGACA ATGTAGGACC CTACATGCCT GTGGATGTTG ACATGTTACA TTTGCGCAGC 
AAATCAGATC CTGGAAAAAC TGTATTGGTG TCCTCGGAGA GCAAAGAGGC CAGATACAAT 
GTTGTGCCTG GATCTCAGCA TGCTCCCAGA CACTTGATCT CTGATCCAGA TGTCCTAATG 
TATATGGAAA CAGAGAAGCA CTGCCAAGGA AATGGAATGG GGGAGAAAAC CACCAAGCAA 
GGCAGCTCTA GGACCTACTC GTCTATACAT TCACATCAAT CCCAGCTTCC ACCACTAAGC 
CGGCAGCACT TACCAGAGGG TGGCCATCAT GACCCAAAGT TTGAACCAGG GGAAAAGCAA 
CTGAGTGGTA AACATTGGCA GGAACATTCG GACAGCAGGA GTGCTCAAGC ACGTTATGAA 
AGGTCTGATT TGGATCGCCA CATATGCAGG GTGAAGGCCA CTAGCAGTGC CAGTGAAGAT 
GAACAAGCTG CTCCTGTGAA GCCGGCTCCT CCACCTAAGC CCGAGAGATC CCATAGTGTT 
CGAGAGCGGC ACCATTACAA CCAGTCCAGT CTTCCTGCAT ATTTACCAGA CAATTCAGAT 
AGAGACCACA GTGGAACATA CTCCCATCAG TCACAGGGGC AGAGACAAAG CACCGTTACT 
TTGCAGTCGC ATTACGACAA CCTGGATGAC TATCATCCAG TACCTCAATC CCAAACCACG 
CTAGCAAATC GGGGTTGTTC CAGTTCCTAT CATAGCCCCG GTTTCTCAAC TCCACACAGT 
AATCGTGCAT ACTCCACTGC CTTGGGACAG GGTGCCTTCA TACAAGCTGA GCTTTCAGTG 
CAAAGGCCAG AGACTGAGAT AAATGCTGAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32b ( XM_009295415.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293690.1 Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X5, mRNA.

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CloneID	ODa45742
Clone ID Related Accession (Same CDS sequence)	XM_009295415.2 , XM_009295415.3
Accession Version	XM_009295415.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho GTPase-activating protein 32 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295415.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGT CTCTTTTCTC CCCTCAGGGG CCTGAGGCCT 
GGACGGCACC CTGAAGAAGA CGATATCGTG CCAGAGCTTG CCAGGAGCAT CCACCCCCGA 
GAGCGACCTG ACTGGGAGGA AACCATCAGC GCAATGGCAC GAAGTGCTGA AATTCCAGAG 
CTGAGCTCTG AGCCGCTCAT GCGCTCCTGC AGCAGCACCG CCAGTATGAA GGTGAAGAAT 
GTCAAAAAGC TGTCATTCAC CAAGGGCCAT TTTCCAAAGT TAGCTGAATG TGCCCACTTC 
CACTATGAAA ACGTTGATTT TGGAACAATA CAGTTAACCC TCGCAGATGA GCAGACTGAA 
GTGACACGGA ATGGCCTGGA GTCAAAAGAG CCTGTGTATT TGGTTCAGAT CTACTGTCAG 
GGACGAAGCT GGATCGTGAG ACGAAGCTAT GAAGATTTCC GAGTCTTGGA TAAGCATCTC 
CATCTCTGCA TCTATGACCG CCGATTTTCC CAGCTCCCTG AGCTGCCCCG TTTCGACAGC 
CTGAGGGAGA GAGCGGAGGC AGTTTCCCAG ATGCTGATGG CTTACCTCTC CCGTCTCTCA 
GCCATTGCTG ATAACAAGAT CAATTGTGGG CCGGCCCTCA CGTGGATGGA GGTTGACAAC 
AAAGGGAATC ATTTGCTGGT GCATGAAGAA TCATCTATAA ATGTTCCAGC TATCGCAGCG 
GCCCACGTGA TCAAGCGCTA CATCGCACAG GCAGCTGATG AGCTGTCATT TGAGGTGGGG 
GATATTGTTT CTGTCATTGA CATGCCACCC AAAGAGGACA CCACCTGGTG GAGGGGAAAA 
CATGGTTTCC AGGTTGGCTT CTTTCCCAGC GAGTGTGTGG AGCTGATAAA CGACAAGGTC 
CCGCAGTCTG TCACCAACTC TGTGCCAAAA CCAGTATCCA AGAAACACGG GAAACTGATC 
ACTTTTCTGC GCACATTCAT GAAGTCCAGG CCGACCAAGC AGAAGCTGAA GCAAAGAGGT 
ATCCTCAGGG AGAGGGTGTT CGGCTGTGAC CTAGGAGAGC ATCTCCTCAA CTCTGGCCAT 
GATGTCCCCC AGGTACTCAA GAGCTGCACC GAGTTCATTG AGAAGCATGG AGTGGTTGAT 
GGAATGTATC GTCTCTCTGG GATCGCCTCC AACATTCAGA AGCTGCGGCA CGAGTTTGAT 
TCTGAACAGA TCCCAGATCT GACAAAAGAT GTTTACATTC AGGATATACA CTGCGTGGGC 
TCCCTGTGCA AGCTCTACTT TCGAGAGCTT CCCAACCCAC TTCTCACCTA CCAGCTGTAC 
GAGAAATTCT CGGAGGCTGT GTCAGCAGCC ACAGACGAGG AGAGGCTGAT CAAAATACAT 
GACGTCATTC AGCAGCTGCC GCCTCCGCAT TATAGAACCC TTGAGTTTCT TATGAGGCAT 
CTTTCACACT TGGCAACCTT CAGCTATGTA ACGAACATGC ATACTAAAAA TTTGGCCATC 
GTTTGGGCCC CCAACTTACT GAGGTCAAAG CAAATAGAAT CTGCTTGCTT CAGTGGGACA 
GCAGCCTTCA TGGAAGTACG AATACAATCG GTTGTGGTGG AGTTCATCCT GAATCATGTG 
GATGTTCTCT TCAGTCCCAA ACTGAGCTCT CTTATTCGAG AGGGAACAGG ACACAACTCT 
TTGTCTCGGC CTAAGTCTTT GCTAGTACCG TCTCCATCCA CAAAGCTGCT AACTCTAGAA 
GAGGCACAGG CACGCACACA GGCCCAGATC AACTCACCCA TCACTGCAGA CAGCAAGTAC 
ATAGAGGTCG GAGAGGGTCC TGCAGCATTA CAAGGAAAAT TCCACACAGT CATAGAATTC 
CCTACTGAGA GGAAGAGGCA GCCCATGAAG TCTAAGAAGT CTCCAGTTGG CAGTTGGCGT 
TCTTTTTTCA ACCTGGGCAA GTCTTCTTCA ATGTCGAAAC GCAAACTACA GCGTAATCCC 
AGTGAACCAA ATGAACTCAA GGCTATGGCA CTGGCTGGAG GAAGAGGAGA CACTGGTACT 
CTTCGGTCAG CCAAAAGTGA AGAGTCTCTC ACCTCTCTAC ATAATGTTGA AGGAGAGTCT 
AAAGTGTATA GGCCCCGCAG ACCTCGCTCC AGCAGTGATG CCCTCTCTGC TTCATTCAAT 
GGTGACCTTC TGGATACCAG ACAGCACTGT AACTCGTACG ACAACCTAGG TCATGGAGAC 
AGTGATGGGG ATGATGGTCC AATCTGTGTG CCTGCTCTCA TCTCCCCACC ACGCTCCGCT 
GATGATGTGG ATCTGAGCCC CCCTGACATT GGCATGGCCT CTTTAGACTT TGACCCCATG 
TCTTTCCAAT GTAGCCTTCC TCTGGCAGAA ACTTCGATTT TCTCAATTGA CGCAGATACA 
AGCAGTCTAA AAAGGAGCCC TGCCAGTGGC TCAGAGTCAG TCTCGCCAGT CCGAGCTAAA 
GTTACGAGCC GGTTTTTGTC TCCAGACCAC AGTACAGCCT TAAAGGATAA ACATAAACTG 
CTCCTTACAT CTTTCTCTGA GAAAACTCCA GTTCAGTCTG TTGAAAGCAC CGAGATGTCA 
GCAGTTGTTT CTCCTCCAAG CATCTCTGAA TCAGCTGCCT CCGTGATAAT GAAAGGACCA 
GAGAGCACTG TGCTTCAGCC CTCTTCCCCT CCATCTCCAC TCGACTCTCC TACAAAATTG 
AGCTCTCTGT CAAGAACCAC CGATCTGCCC CTGAGTGAAG CCATTCAACA AGAGCTTCTT 
TCTAAAGCTG CTACCTTTGA GAGTAAAGAC ATATCGAGCA TTGAATGTTC ACAACCACCA 
CAGGTCACCT GCTCTCAAGA ACAGCCAGGA GCCGTAGCTT TGGACTCTCC AAAGGGCAAT 
GTCCCTGTCA GCTCTGTGTC CCTCGTCCCT CCCCCTCCCC CACCAAAGAA CGCTGCCCGC 
ATGCTTGCAC TAGCTCTAGC TGAATCTGCC CAGCAGGCCA CCATTCTTTC TCAGAAGCAA 
TCCTCTGGAC CCCCTACACC AGTATCCCCT CTTAGGCCTC AAGAGTTATT GGAGACCCTG 
GACTGGCCGC TGCCTTCTTC CTTGCCATCA CAGCCATCCG AGGAGGAAAC TGCAGTAGAA 
TCATCAAATA AATCGGTTCC TTTGTCTTCA CCACCCTCCA CTCCACCTGA AAAAATGACT 
CGCTCAGTCA GTCTGCACCT CACCACAGCT GATAGTGGGA AGCCATCCAG CATTTCATGC 
AACAGCCAGG ATGTAATCTC ACCAGAGACC CCAAGCCAGA GTGCTATGCC CTCTTGTCTG 
ACTTCTCATG TTTCTCAAAC ACAAAAAAGT CCAGAAAAAC AGCAGCCTGC TGTCGGTCAG 
ACACCTGTGA GCACAGCAAA AACCACCACC ACAACCACCA CTTCAACCAT CAGCAGTAAG 
GATGCTGTCA TTCAACAACC CACCTCTGAG ATCCATATGC CTGAAACAGT CCTGCCACCA 
AAACCAAACA AGGTGCCAGA TCAACCAGTG GCAATTCTTC AGCCTCAGCC TCAAATGCAG 
ACGCAACCTC ATACTGCATT TCGGTCTCAA CCTCAAGTTC AGCCTCTTCC TCAGTCACAA 
GCACAGCCAC AGTCTCAGCC CAGATCACAG CCCAATGCTA GAGTTGCACC AACCCTGGCA 
GCACCTCATG ATCCTGTGGA AAAACCATGG GAAGCCATAA AGCCTGTCCA TCCAAAAGGA 
GATAGTGCTT CTCAACAACA CCACACTCAG GGAGTGACAC CACCAACACC CCCTATTCGC 
TCGATTGAAA GTAAACTAGC AGCAGCTGCA CTTTGCAACA CCGAGGCTGC AAACCCTGCC 
AACTTTCATG CCATGTTAGA GACATCCATG AGGGGATCAG TAGAGGAGAC TCCCCCACAG 
TCTCATCCGC CTCACCGCAG ATCGTCTACT CATCAGTCAG GTTATCTTTA TCACACAAAA 
GGGGAGGCAG CCTTACTGGA CCCCACCACA GAGGCATATT ACCATCAGCG GGTAACCCCT 
TCAGGTCAGG CTACAGTGGG GTACCATTAC CGGCCAGAGA GTGTTCCACC TCACATTTCT 
TACGTGTCAA AATCAGAGCC ACACATTTCC TACAGAGGTC ATGGGGATGG TCGATACAAT 
ACAATTGGAC CTAGATCCTA CCACCAGTCT ATCAAATCTC GTGGTTCACT TAGAAGTGAG 
TACATCTCTC CAAGCTCATT ACACCACTCC CATAGCTACA GCCAAGCAGA TGGACCAACC 
GTCTACCCAA CAATTCGCAG GGTCCACTCC TTACATGTGC CTCCCACTGC TATTCGCTCA 
ATGCCAGTCA CCAGAACTGA GGTACCACCA GATGATGAGT TATTTTACTA CCAGCGCCCA 
ATCTACCACT GCAAGTCTCA CCAACCATCA TCTCAAGTCG ACTATCACGT CACACAACTG 
CAGCCTTATT TTGAAAATGG AAGAGTTCAG TACCGCTACA GCCCCTACTC TGGCTCTCAC 
GTTCTGGAAT CCCCTTTCTA TGATGTGGAC CATTATGGTA CTATCCGCTT AAGGCACGTC 
CATTCCTTCA GTGGTCGGGA CAGTGCCCCT CTCCTTCGAT CTGGAGCAAA ATCTGGAGGC 
TACCACTACC TTTCACGGCA TGTTATTCCT TTAAAGGAGC ATGGCTTCAT AAGCAGGGAC 
ATGCCACCTT ACAGTGGTTC AAAAGGGAGT ATTTACTTTG CATGGGACCC AGAGGAGGCA 
GAAAGGCTGC GCATACACTC CATCCGTAGG GAAAGCAGAG CAAGGCAAAA GGTTAAGGGT 
TCTGTTATGT CTCAATACGA CAATGTAGGA CCCTACATGC CTGTGGATGT TGACATGTTA 
CATTTGCGCA GCAAATCAGA TCCTGGAAAA ACTGTATTGG TGTCCTCGGA GAGCAAAGAG 
GCCAGATACA ATGTTGTGCC TGGATCTCAG CATGCTCCCA GACACTTGAT CTCTGATCCA 
GATGTCCTAA TGTATATGGA AACAGAGAAG CACTGCCAAG GAAATGGAAT GGGGGAGAAA 
ACCACCAAGC AAGGCAGCTC TAGGACCTAC TCGTCTATAC ATTCACATCA ATCCCAGCTT 
CCACCACTAA GCCGGCAGCA CTTACCAGAG GGTGGCCATC ATGACCCAAA GTTTGAACCA 
GGGGAAAAGC AACTGAGTGG TAAACATTGG CAGGAACATT CGGACAGCAG GAGTGCTCAA 
GCACGTTATG AAAGGTCTGA TTTGGATCGC CACATATGCA GGGTGAAGGC CACTAGCAGT 
GCCAGTGAAG ATGAACAAGC TGCTCCTGTG AAGCCGGCTC CTCCACCTAA GCCCGAGAGA 
TCCCATAGTG TTCGAGAGCG GCACCATTAC AACCAGTCCA GTCTTCCTGC ATATTTACCA 
GACAATTCAG ATAGAGACCA CAGTGGAACA TACTCCCATC AGTCACAGGG GCAGAGACAA 
AGCACCGTTA CTTTGCAGTC GCATTACGAC AACCTGGATG ACTATCATCC AGTACCTCAA 
TCCCAAACCA CGCTAGCAAA TCGGGGTTGT TCCAGTTCCT ATCATAGCCC CGGTTTCTCA 
ACTCCACACA GTAATCGTGC ATACTCCACT GCCTTGGGAC AGGGTGCCTT CATACAAGCT 
GAGCTTTCAG TGCAAAGGCC AGAGACTGAG ATAAATGCTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293690.1
CDS	524..6268
Translation	MEAGSGAAAVVGSAALGLLGATGSHDISDSLFSPLRGLRPGRHPEEDDIVPELARSIHPRERPDWEETISAMARSAEIPELSSEPLMRSCSSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGTIQLTLADEQTEVTRNGLESKEPVYLVQIYCQGRSWIVRRSYEDFRVLDKHLHLCIYDRRFSQLPELPRFDSLRERAEAVSQMLMAYLSRLSAIADNKINCGPALTWMEVDNKGNHLLVHEESSINVPAIAAAHVIKRYIAQAADELSFEVGDIVSVIDMPPKEDTTWWRGKHGFQVGFFPSECVELINDKVPQSVTNSVPKPVSKKHGKLITFLRTFMKSRPTKQKLKQRGILRERVFGCDLGEHLLNSGHDVPQVLKSCTEFIEKHGVVDGMYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSEAVSAATDEERLIKIHDVIQQLPPPHYRTLEFLMRHLSHLATFSYVTNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVDVLFSPKLSSLIREGTGHNSLSRPKSLLVPSPSTKLLTLEEAQARTQAQINSPITADSKYIEVGEGPAALQGKFHTVIEFPTERKRQPMKSKKSPVGSWRSFFNLGKSSSMSKRKLQRNPSEPNELKAMALAGGRGDTGTLRSAKSEESLTSLHNVEGESKVYRPRRPRSSSDALSASFNGDLLDTRQHCNSYDNLGHGDSDGDDGPICVPALISPPRSADDVDLSPPDIGMASLDFDPMSFQCSLPLAETSIFSIDADTSSLKRSPASGSESVSPVRAKVTSRFLSPDHSTALKDKHKLLLTSFSEKTPVQSVESTEMSAVVSPPSISESAASVIMKGPESTVLQPSSPPSPLDSPTKLSSLSRTTDLPLSEAIQQELLSKAATFESKDISSIECSQPPQVTCSQEQPGAVALDSPKGNVPVSSVSLVPPPPPPKNAARMLALALAESAQQATILSQKQSSGPPTPVSPLRPQELLETLDWPLPSSLPSQPSEEETAVESSNKSVPLSSPPSTPPEKMTRSVSLHLTTADSGKPSSISCNSQDVISPETPSQSAMPSCLTSHVSQTQKSPEKQQPAVGQTPVSTAKTTTTTTTSTISSKDAVIQQPTSEIHMPETVLPPKPNKVPDQPVAILQPQPQMQTQPHTAFRSQPQVQPLPQSQAQPQSQPRSQPNARVAPTLAAPHDPVEKPWEAIKPVHPKGDSASQQHHTQGVTPPTPPIRSIESKLAAAALCNTEAANPANFHAMLETSMRGSVEETPPQSHPPHRRSSTHQSGYLYHTKGEAALLDPTTEAYYHQRVTPSGQATVGYHYRPESVPPHISYVSKSEPHISYRGHGDGRYNTIGPRSYHQSIKSRGSLRSEYISPSSLHHSHSYSQADGPTVYPTIRRVHSLHVPPTAIRSMPVTRTEVPPDDELFYYQRPIYHCKSHQPSSQVDYHVTQLQPYFENGRVQYRYSPYSGSHVLESPFYDVDHYGTIRLRHVHSFSGRDSAPLLRSGAKSGGYHYLSRHVIPLKEHGFISRDMPPYSGSKGSIYFAWDPEEAERLRIHSIRRESRARQKVKGSVMSQYDNVGPYMPVDVDMLHLRSKSDPGKTVLVSSESKEARYNVVPGSQHAPRHLISDPDVLMYMETEKHCQGNGMGEKTTKQGSSRTYSSIHSHQSQLPPLSRQHLPEGGHHDPKFEPGEKQLSGKHWQEHSDSRSAQARYERSDLDRHICRVKATSSASEDEQAAPVKPAPPPKPERSHSVRERHHYNQSSLPAYLPDNSDRDHSGTYSHQSQGQRQSTVTLQSHYDNLDDYHPVPQSQTTLANRGCSSSYHSPGFSTPHSNRAYSTALGQGAFIQAELSVQRPETEINAE

Target ORF information:

RefSeq Version	XM_009295415.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295415.2

ORF Insert Sequence:

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGT CTCTTTTCTC CCCTCAGGGG CCTGAGGCCT 
GGACGGCACC CTGAAGAAGA CGATATCGTG CCAGAGCTTG CCAGGAGCAT CCACCCCCGA 
GAGCGACCTG ACTGGGAGGA AACCATCAGC GCAATGGCAC GAAGTGCTGA AATTCCAGAG 
CTGAGCTCTG AGCCGCTCAT GCGCTCCTGC AGCAGCACCG CCAGTATGAA GGTGAAGAAT 
GTCAAAAAGC TGTCATTCAC CAAGGGCCAT TTTCCAAAGT TAGCTGAATG TGCCCACTTC 
CACTATGAAA ACGTTGATTT TGGAACAATA CAGTTAACCC TCGCAGATGA GCAGACTGAA 
GTGACACGGA ATGGCCTGGA GTCAAAAGAG CCTGTGTATT TGGTTCAGAT CTACTGTCAG 
GGACGAAGCT GGATCGTGAG ACGAAGCTAT GAAGATTTCC GAGTCTTGGA TAAGCATCTC 
CATCTCTGCA TCTATGACCG CCGATTTTCC CAGCTCCCTG AGCTGCCCCG TTTCGACAGC 
CTGAGGGAGA GAGCGGAGGC AGTTTCCCAG ATGCTGATGG CTTACCTCTC CCGTCTCTCA 
GCCATTGCTG ATAACAAGAT CAATTGTGGG CCGGCCCTCA CGTGGATGGA GGTTGACAAC 
AAAGGGAATC ATTTGCTGGT GCATGAAGAA TCATCTATAA ATGTTCCAGC TATCGCAGCG 
GCCCACGTGA TCAAGCGCTA CATCGCACAG GCAGCTGATG AGCTGTCATT TGAGGTGGGG 
GATATTGTTT CTGTCATTGA CATGCCACCC AAAGAGGACA CCACCTGGTG GAGGGGAAAA 
CATGGTTTCC AGGTTGGCTT CTTTCCCAGC GAGTGTGTGG AGCTGATAAA CGACAAGGTC 
CCGCAGTCTG TCACCAACTC TGTGCCAAAA CCAGTATCCA AGAAACACGG GAAACTGATC 
ACTTTTCTGC GCACATTCAT GAAGTCCAGG CCGACCAAGC AGAAGCTGAA GCAAAGAGGT 
ATCCTCAGGG AGAGGGTGTT CGGCTGTGAC CTAGGAGAGC ATCTCCTCAA CTCTGGCCAT 
GATGTCCCCC AGGTACTCAA GAGCTGCACC GAGTTCATTG AGAAGCATGG AGTGGTTGAT 
GGAATGTATC GTCTCTCTGG GATCGCCTCC AACATTCAGA AGCTGCGGCA CGAGTTTGAT 
TCTGAACAGA TCCCAGATCT GACAAAAGAT GTTTACATTC AGGATATACA CTGCGTGGGC 
TCCCTGTGCA AGCTCTACTT TCGAGAGCTT CCCAACCCAC TTCTCACCTA CCAGCTGTAC 
GAGAAATTCT CGGAGGCTGT GTCAGCAGCC ACAGACGAGG AGAGGCTGAT CAAAATACAT 
GACGTCATTC AGCAGCTGCC GCCTCCGCAT TATAGAACCC TTGAGTTTCT TATGAGGCAT 
CTTTCACACT TGGCAACCTT CAGCTATGTA ACGAACATGC ATACTAAAAA TTTGGCCATC 
GTTTGGGCCC CCAACTTACT GAGGTCAAAG CAAATAGAAT CTGCTTGCTT CAGTGGGACA 
GCAGCCTTCA TGGAAGTACG AATACAATCG GTTGTGGTGG AGTTCATCCT GAATCATGTG 
GATGTTCTCT TCAGTCCCAA ACTGAGCTCT CTTATTCGAG AGGGAACAGG ACACAACTCT 
TTGTCTCGGC CTAAGTCTTT GCTAGTACCG TCTCCATCCA CAAAGCTGCT AACTCTAGAA 
GAGGCACAGG CACGCACACA GGCCCAGATC AACTCACCCA TCACTGCAGA CAGCAAGTAC 
ATAGAGGTCG GAGAGGGTCC TGCAGCATTA CAAGGAAAAT TCCACACAGT CATAGAATTC 
CCTACTGAGA GGAAGAGGCA GCCCATGAAG TCTAAGAAGT CTCCAGTTGG CAGTTGGCGT 
TCTTTTTTCA ACCTGGGCAA GTCTTCTTCA ATGTCGAAAC GCAAACTACA GCGTAATCCC 
AGTGAACCAA ATGAACTCAA GGCTATGGCA CTGGCTGGAG GAAGAGGAGA CACTGGTACT 
CTTCGGTCAG CCAAAAGTGA AGAGTCTCTC ACCTCTCTAC ATAATGTTGA AGGAGAGTCT 
AAAGTGTATA GGCCCCGCAG ACCTCGCTCC AGCAGTGATG CCCTCTCTGC TTCATTCAAT 
GGTGACCTTC TGGATACCAG ACAGCACTGT AACTCGTACG ACAACCTAGG TCATGGAGAC 
AGTGATGGGG ATGATGGTCC AATCTGTGTG CCTGCTCTCA TCTCCCCACC ACGCTCCGCT 
GATGATGTGG ATCTGAGCCC CCCTGACATT GGCATGGCCT CTTTAGACTT TGACCCCATG 
TCTTTCCAAT GTAGCCTTCC TCTGGCAGAA ACTTCGATTT TCTCAATTGA CGCAGATACA 
AGCAGTCTAA AAAGGAGCCC TGCCAGTGGC TCAGAGTCAG TCTCGCCAGT CCGAGCTAAA 
GTTACGAGCC GGTTTTTGTC TCCAGACCAC AGTACAGCCT TAAAGGATAA ACATAAACTG 
CTCCTTACAT CTTTCTCTGA GAAAACTCCA GTTCAGTCTG TTGAAAGCAC CGAGATGTCA 
GCAGTTGTTT CTCCTCCAAG CATCTCTGAA TCAGCTGCCT CCGTGATAAT GAAAGGACCA 
GAGAGCACTG TGCTTCAGCC CTCTTCCCCT CCATCTCCAC TCGACTCTCC TACAAAATTG 
AGCTCTCTGT CAAGAACCAC CGATCTGCCC CTGAGTGAAG CCATTCAACA AGAGCTTCTT 
TCTAAAGCTG CTACCTTTGA GAGTAAAGAC ATATCGAGCA TTGAATGTTC ACAACCACCA 
CAGGTCACCT GCTCTCAAGA ACAGCCAGGA GCCGTAGCTT TGGACTCTCC AAAGGGCAAT 
GTCCCTGTCA GCTCTGTGTC CCTCGTCCCT CCCCCTCCCC CACCAAAGAA CGCTGCCCGC 
ATGCTTGCAC TAGCTCTAGC TGAATCTGCC CAGCAGGCCA CCATTCTTTC TCAGAAGCAA 
TCCTCTGGAC CCCCTACACC AGTATCCCCT CTTAGGCCTC AAGAGTTATT GGAGACCCTG 
GACTGGCCGC TGCCTTCTTC CTTGCCATCA CAGCCATCCG AGGAGGAAAC TGCAGTAGAA 
TCATCAAATA AATCGGTTCC TTTGTCTTCA CCACCCTCCA CTCCACCTGA AAAAATGACT 
CGCTCAGTCA GTCTGCACCT CACCACAGCT GATAGTGGGA AGCCATCCAG CATTTCATGC 
AACAGCCAGG ATGTAATCTC ACCAGAGACC CCAAGCCAGA GTGCTATGCC CTCTTGTCTG 
ACTTCTCATG TTTCTCAAAC ACAAAAAAGT CCAGAAAAAC AGCAGCCTGC TGTCGGTCAG 
ACACCTGTGA GCACAGCAAA AACCACCACC ACAACCACCA CTTCAACCAT CAGCAGTAAG 
GATGCTGTCA TTCAACAACC CACCTCTGAG ATCCATATGC CTGAAACAGT CCTGCCACCA 
AAACCAAACA AGGTGCCAGA TCAACCAGTG GCAATTCTTC AGCCTCAGCC TCAAATGCAG 
ACGCAACCTC ATACTGCATT TCGGTCTCAA CCTCAAGTTC AGCCTCTTCC TCAGTCACAA 
GCACAGCCAC AGTCTCAGCC CAGATCACAG CCCAATGCTA GAGTTGCACC AACCCTGGCA 
GCACCTCATG ATCCTGTGGA AAAACCATGG GAAGCCATAA AGCCTGTCCA TCCAAAAGGA 
GATAGTGCTT CTCAACAACA CCACACTCAG GGAGTGACAC CACCAACACC CCCTATTCGC 
TCGATTGAAA GTAAACTAGC AGCAGCTGCA CTTTGCAACA CCGAGGCTGC AAACCCTGCC 
AACTTTCATG CCATGTTAGA GACATCCATG AGGGGATCAG TAGAGGAGAC TCCCCCACAG 
TCTCATCCGC CTCACCGCAG ATCGTCTACT CATCAGTCAG GTTATCTTTA TCACACAAAA 
GGGGAGGCAG CCTTACTGGA CCCCACCACA GAGGCATATT ACCATCAGCG GGTAACCCCT 
TCAGGTCAGG CTACAGTGGG GTACCATTAC CGGCCAGAGA GTGTTCCACC TCACATTTCT 
TACGTGTCAA AATCAGAGCC ACACATTTCC TACAGAGGTC ATGGGGATGG TCGATACAAT 
ACAATTGGAC CTAGATCCTA CCACCAGTCT ATCAAATCTC GTGGTTCACT TAGAAGTGAG 
TACATCTCTC CAAGCTCATT ACACCACTCC CATAGCTACA GCCAAGCAGA TGGACCAACC 
GTCTACCCAA CAATTCGCAG GGTCCACTCC TTACATGTGC CTCCCACTGC TATTCGCTCA 
ATGCCAGTCA CCAGAACTGA GGTACCACCA GATGATGAGT TATTTTACTA CCAGCGCCCA 
ATCTACCACT GCAAGTCTCA CCAACCATCA TCTCAAGTCG ACTATCACGT CACACAACTG 
CAGCCTTATT TTGAAAATGG AAGAGTTCAG TACCGCTACA GCCCCTACTC TGGCTCTCAC 
GTTCTGGAAT CCCCTTTCTA TGATGTGGAC CATTATGGTA CTATCCGCTT AAGGCACGTC 
CATTCCTTCA GTGGTCGGGA CAGTGCCCCT CTCCTTCGAT CTGGAGCAAA ATCTGGAGGC 
TACCACTACC TTTCACGGCA TGTTATTCCT TTAAAGGAGC ATGGCTTCAT AAGCAGGGAC 
ATGCCACCTT ACAGTGGTTC AAAAGGGAGT ATTTACTTTG CATGGGACCC AGAGGAGGCA 
GAAAGGCTGC GCATACACTC CATCCGTAGG GAAAGCAGAG CAAGGCAAAA GGTTAAGGGT 
TCTGTTATGT CTCAATACGA CAATGTAGGA CCCTACATGC CTGTGGATGT TGACATGTTA 
CATTTGCGCA GCAAATCAGA TCCTGGAAAA ACTGTATTGG TGTCCTCGGA GAGCAAAGAG 
GCCAGATACA ATGTTGTGCC TGGATCTCAG CATGCTCCCA GACACTTGAT CTCTGATCCA 
GATGTCCTAA TGTATATGGA AACAGAGAAG CACTGCCAAG GAAATGGAAT GGGGGAGAAA 
ACCACCAAGC AAGGCAGCTC TAGGACCTAC TCGTCTATAC ATTCACATCA ATCCCAGCTT 
CCACCACTAA GCCGGCAGCA CTTACCAGAG GGTGGCCATC ATGACCCAAA GTTTGAACCA 
GGGGAAAAGC AACTGAGTGG TAAACATTGG CAGGAACATT CGGACAGCAG GAGTGCTCAA 
GCACGTTATG AAAGGTCTGA TTTGGATCGC CACATATGCA GGGTGAAGGC CACTAGCAGT 
GCCAGTGAAG ATGAACAAGC TGCTCCTGTG AAGCCGGCTC CTCCACCTAA GCCCGAGAGA 
TCCCATAGTG TTCGAGAGCG GCACCATTAC AACCAGTCCA GTCTTCCTGC ATATTTACCA 
GACAATTCAG ATAGAGACCA CAGTGGAACA TACTCCCATC AGTCACAGGG GCAGAGACAA 
AGCACCGTTA CTTTGCAGTC GCATTACGAC AACCTGGATG ACTATCATCC AGTACCTCAA 
TCCCAAACCA CGCTAGCAAA TCGGGGTTGT TCCAGTTCCT ATCATAGCCC CGGTTTCTCA 
ACTCCACACA GTAATCGTGC ATACTCCACT GCCTTGGGAC AGGGTGCCTT CATACAAGCT 
GAGCTTTCAG TGCAAAGGCC AGAGACTGAG ATAAATGCTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

arhgap32b ( XM_009295415.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293690.1 Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X7, mRNA.

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CloneID	ODa45742
Clone ID Related Accession (Same CDS sequence)	XM_009295415.2 , XM_009295415.3
Accession Version	XM_009295415.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho GTPase-activating protein 32 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009295415.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGT CTCTTTTCTC CCCTCAGGGG CCTGAGGCCT 
GGACGGCACC CTGAAGAAGA CGATATCGTG CCAGAGCTTG CCAGGAGCAT CCACCCCCGA 
GAGCGACCTG ACTGGGAGGA AACCATCAGC GCAATGGCAC GAAGTGCTGA AATTCCAGAG 
CTGAGCTCTG AGCCGCTCAT GCGCTCCTGC AGCAGCACCG CCAGTATGAA GGTGAAGAAT 
GTCAAAAAGC TGTCATTCAC CAAGGGCCAT TTTCCAAAGT TAGCTGAATG TGCCCACTTC 
CACTATGAAA ACGTTGATTT TGGAACAATA CAGTTAACCC TCGCAGATGA GCAGACTGAA 
GTGACACGGA ATGGCCTGGA GTCAAAAGAG CCTGTGTATT TGGTTCAGAT CTACTGTCAG 
GGACGAAGCT GGATCGTGAG ACGAAGCTAT GAAGATTTCC GAGTCTTGGA TAAGCATCTC 
CATCTCTGCA TCTATGACCG CCGATTTTCC CAGCTCCCTG AGCTGCCCCG TTTCGACAGC 
CTGAGGGAGA GAGCGGAGGC AGTTTCCCAG ATGCTGATGG CTTACCTCTC CCGTCTCTCA 
GCCATTGCTG ATAACAAGAT CAATTGTGGG CCGGCCCTCA CGTGGATGGA GGTTGACAAC 
AAAGGGAATC ATTTGCTGGT GCATGAAGAA TCATCTATAA ATGTTCCAGC TATCGCAGCG 
GCCCACGTGA TCAAGCGCTA CATCGCACAG GCAGCTGATG AGCTGTCATT TGAGGTGGGG 
GATATTGTTT CTGTCATTGA CATGCCACCC AAAGAGGACA CCACCTGGTG GAGGGGAAAA 
CATGGTTTCC AGGTTGGCTT CTTTCCCAGC GAGTGTGTGG AGCTGATAAA CGACAAGGTC 
CCGCAGTCTG TCACCAACTC TGTGCCAAAA CCAGTATCCA AGAAACACGG GAAACTGATC 
ACTTTTCTGC GCACATTCAT GAAGTCCAGG CCGACCAAGC AGAAGCTGAA GCAAAGAGGT 
ATCCTCAGGG AGAGGGTGTT CGGCTGTGAC CTAGGAGAGC ATCTCCTCAA CTCTGGCCAT 
GATGTCCCCC AGGTACTCAA GAGCTGCACC GAGTTCATTG AGAAGCATGG AGTGGTTGAT 
GGAATGTATC GTCTCTCTGG GATCGCCTCC AACATTCAGA AGCTGCGGCA CGAGTTTGAT 
TCTGAACAGA TCCCAGATCT GACAAAAGAT GTTTACATTC AGGATATACA CTGCGTGGGC 
TCCCTGTGCA AGCTCTACTT TCGAGAGCTT CCCAACCCAC TTCTCACCTA CCAGCTGTAC 
GAGAAATTCT CGGAGGCTGT GTCAGCAGCC ACAGACGAGG AGAGGCTGAT CAAAATACAT 
GACGTCATTC AGCAGCTGCC GCCTCCGCAT TATAGAACCC TTGAGTTTCT TATGAGGCAT 
CTTTCACACT TGGCAACCTT CAGCTATGTA ACGAACATGC ATACTAAAAA TTTGGCCATC 
GTTTGGGCCC CCAACTTACT GAGGTCAAAG CAAATAGAAT CTGCTTGCTT CAGTGGGACA 
GCAGCCTTCA TGGAAGTACG AATACAATCG GTTGTGGTGG AGTTCATCCT GAATCATGTG 
GATGTTCTCT TCAGTCCCAA ACTGAGCTCT CTTATTCGAG AGGGAACAGG ACACAACTCT 
TTGTCTCGGC CTAAGTCTTT GCTAGTACCG TCTCCATCCA CAAAGCTGCT AACTCTAGAA 
GAGGCACAGG CACGCACACA GGCCCAGATC AACTCACCCA TCACTGCAGA CAGCAAGTAC 
ATAGAGGTCG GAGAGGGTCC TGCAGCATTA CAAGGAAAAT TCCACACAGT CATAGAATTC 
CCTACTGAGA GGAAGAGGCA GCCCATGAAG TCTAAGAAGT CTCCAGTTGG CAGTTGGCGT 
TCTTTTTTCA ACCTGGGCAA GTCTTCTTCA ATGTCGAAAC GCAAACTACA GCGTAATCCC 
AGTGAACCAA ATGAACTCAA GGCTATGGCA CTGGCTGGAG GAAGAGGAGA CACTGGTACT 
CTTCGGTCAG CCAAAAGTGA AGAGTCTCTC ACCTCTCTAC ATAATGTTGA AGGAGAGTCT 
AAAGTGTATA GGCCCCGCAG ACCTCGCTCC AGCAGTGATG CCCTCTCTGC TTCATTCAAT 
GGTGACCTTC TGGATACCAG ACAGCACTGT AACTCGTACG ACAACCTAGG TCATGGAGAC 
AGTGATGGGG ATGATGGTCC AATCTGTGTG CCTGCTCTCA TCTCCCCACC ACGCTCCGCT 
GATGATGTGG ATCTGAGCCC CCCTGACATT GGCATGGCCT CTTTAGACTT TGACCCCATG 
TCTTTCCAAT GTAGCCTTCC TCTGGCAGAA ACTTCGATTT TCTCAATTGA CGCAGATACA 
AGCAGTCTAA AAAGGAGCCC TGCCAGTGGC TCAGAGTCAG TCTCGCCAGT CCGAGCTAAA 
GTTACGAGCC GGTTTTTGTC TCCAGACCAC AGTACAGCCT TAAAGGATAA ACATAAACTG 
CTCCTTACAT CTTTCTCTGA GAAAACTCCA GTTCAGTCTG TTGAAAGCAC CGAGATGTCA 
GCAGTTGTTT CTCCTCCAAG CATCTCTGAA TCAGCTGCCT CCGTGATAAT GAAAGGACCA 
GAGAGCACTG TGCTTCAGCC CTCTTCCCCT CCATCTCCAC TCGACTCTCC TACAAAATTG 
AGCTCTCTGT CAAGAACCAC CGATCTGCCC CTGAGTGAAG CCATTCAACA AGAGCTTCTT 
TCTAAAGCTG CTACCTTTGA GAGTAAAGAC ATATCGAGCA TTGAATGTTC ACAACCACCA 
CAGGTCACCT GCTCTCAAGA ACAGCCAGGA GCCGTAGCTT TGGACTCTCC AAAGGGCAAT 
GTCCCTGTCA GCTCTGTGTC CCTCGTCCCT CCCCCTCCCC CACCAAAGAA CGCTGCCCGC 
ATGCTTGCAC TAGCTCTAGC TGAATCTGCC CAGCAGGCCA CCATTCTTTC TCAGAAGCAA 
TCCTCTGGAC CCCCTACACC AGTATCCCCT CTTAGGCCTC AAGAGTTATT GGAGACCCTG 
GACTGGCCGC TGCCTTCTTC CTTGCCATCA CAGCCATCCG AGGAGGAAAC TGCAGTAGAA 
TCATCAAATA AATCGGTTCC TTTGTCTTCA CCACCCTCCA CTCCACCTGA AAAAATGACT 
CGCTCAGTCA GTCTGCACCT CACCACAGCT GATAGTGGGA AGCCATCCAG CATTTCATGC 
AACAGCCAGG ATGTAATCTC ACCAGAGACC CCAAGCCAGA GTGCTATGCC CTCTTGTCTG 
ACTTCTCATG TTTCTCAAAC ACAAAAAAGT CCAGAAAAAC AGCAGCCTGC TGTCGGTCAG 
ACACCTGTGA GCACAGCAAA AACCACCACC ACAACCACCA CTTCAACCAT CAGCAGTAAG 
GATGCTGTCA TTCAACAACC CACCTCTGAG ATCCATATGC CTGAAACAGT CCTGCCACCA 
AAACCAAACA AGGTGCCAGA TCAACCAGTG GCAATTCTTC AGCCTCAGCC TCAAATGCAG 
ACGCAACCTC ATACTGCATT TCGGTCTCAA CCTCAAGTTC AGCCTCTTCC TCAGTCACAA 
GCACAGCCAC AGTCTCAGCC CAGATCACAG CCCAATGCTA GAGTTGCACC AACCCTGGCA 
GCACCTCATG ATCCTGTGGA AAAACCATGG GAAGCCATAA AGCCTGTCCA TCCAAAAGGA 
GATAGTGCTT CTCAACAACA CCACACTCAG GGAGTGACAC CACCAACACC CCCTATTCGC 
TCGATTGAAA GTAAACTAGC AGCAGCTGCA CTTTGCAACA CCGAGGCTGC AAACCCTGCC 
AACTTTCATG CCATGTTAGA GACATCCATG AGGGGATCAG TAGAGGAGAC TCCCCCACAG 
TCTCATCCGC CTCACCGCAG ATCGTCTACT CATCAGTCAG GTTATCTTTA TCACACAAAA 
GGGGAGGCAG CCTTACTGGA CCCCACCACA GAGGCATATT ACCATCAGCG GGTAACCCCT 
TCAGGTCAGG CTACAGTGGG GTACCATTAC CGGCCAGAGA GTGTTCCACC TCACATTTCT 
TACGTGTCAA AATCAGAGCC ACACATTTCC TACAGAGGTC ATGGGGATGG TCGATACAAT 
ACAATTGGAC CTAGATCCTA CCACCAGTCT ATCAAATCTC GTGGTTCACT TAGAAGTGAG 
TACATCTCTC CAAGCTCATT ACACCACTCC CATAGCTACA GCCAAGCAGA TGGACCAACC 
GTCTACCCAA CAATTCGCAG GGTCCACTCC TTACATGTGC CTCCCACTGC TATTCGCTCA 
ATGCCAGTCA CCAGAACTGA GGTACCACCA GATGATGAGT TATTTTACTA CCAGCGCCCA 
ATCTACCACT GCAAGTCTCA CCAACCATCA TCTCAAGTCG ACTATCACGT CACACAACTG 
CAGCCTTATT TTGAAAATGG AAGAGTTCAG TACCGCTACA GCCCCTACTC TGGCTCTCAC 
GTTCTGGAAT CCCCTTTCTA TGATGTGGAC CATTATGGTA CTATCCGCTT AAGGCACGTC 
CATTCCTTCA GTGGTCGGGA CAGTGCCCCT CTCCTTCGAT CTGGAGCAAA ATCTGGAGGC 
TACCACTACC TTTCACGGCA TGTTATTCCT TTAAAGGAGC ATGGCTTCAT AAGCAGGGAC 
ATGCCACCTT ACAGTGGTTC AAAAGGGAGT ATTTACTTTG CATGGGACCC AGAGGAGGCA 
GAAAGGCTGC GCATACACTC CATCCGTAGG GAAAGCAGAG CAAGGCAAAA GGTTAAGGGT 
TCTGTTATGT CTCAATACGA CAATGTAGGA CCCTACATGC CTGTGGATGT TGACATGTTA 
CATTTGCGCA GCAAATCAGA TCCTGGAAAA ACTGTATTGG TGTCCTCGGA GAGCAAAGAG 
GCCAGATACA ATGTTGTGCC TGGATCTCAG CATGCTCCCA GACACTTGAT CTCTGATCCA 
GATGTCCTAA TGTATATGGA AACAGAGAAG CACTGCCAAG GAAATGGAAT GGGGGAGAAA 
ACCACCAAGC AAGGCAGCTC TAGGACCTAC TCGTCTATAC ATTCACATCA ATCCCAGCTT 
CCACCACTAA GCCGGCAGCA CTTACCAGAG GGTGGCCATC ATGACCCAAA GTTTGAACCA 
GGGGAAAAGC AACTGAGTGG TAAACATTGG CAGGAACATT CGGACAGCAG GAGTGCTCAA 
GCACGTTATG AAAGGTCTGA TTTGGATCGC CACATATGCA GGGTGAAGGC CACTAGCAGT 
GCCAGTGAAG ATGAACAAGC TGCTCCTGTG AAGCCGGCTC CTCCACCTAA GCCCGAGAGA 
TCCCATAGTG TTCGAGAGCG GCACCATTAC AACCAGTCCA GTCTTCCTGC ATATTTACCA 
GACAATTCAG ATAGAGACCA CAGTGGAACA TACTCCCATC AGTCACAGGG GCAGAGACAA 
AGCACCGTTA CTTTGCAGTC GCATTACGAC AACCTGGATG ACTATCATCC AGTACCTCAA 
TCCCAAACCA CGCTAGCAAA TCGGGGTTGT TCCAGTTCCT ATCATAGCCC CGGTTTCTCA 
ACTCCACACA GTAATCGTGC ATACTCCACT GCCTTGGGAC AGGGTGCCTT CATACAAGCT 
GAGCTTTCAG TGCAAAGGCC AGAGACTGAG ATAAATGCTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293690.1
CDS	521..6265
Translation	MEAGSGAAAVVGSAALGLLGATGSHDISDSLFSPLRGLRPGRHPEEDDIVPELARSIHPRERPDWEETISAMARSAEIPELSSEPLMRSCSSTASMKVKNVKKLSFTKGHFPKLAECAHFHYENVDFGTIQLTLADEQTEVTRNGLESKEPVYLVQIYCQGRSWIVRRSYEDFRVLDKHLHLCIYDRRFSQLPELPRFDSLRERAEAVSQMLMAYLSRLSAIADNKINCGPALTWMEVDNKGNHLLVHEESSINVPAIAAAHVIKRYIAQAADELSFEVGDIVSVIDMPPKEDTTWWRGKHGFQVGFFPSECVELINDKVPQSVTNSVPKPVSKKHGKLITFLRTFMKSRPTKQKLKQRGILRERVFGCDLGEHLLNSGHDVPQVLKSCTEFIEKHGVVDGMYRLSGIASNIQKLRHEFDSEQIPDLTKDVYIQDIHCVGSLCKLYFRELPNPLLTYQLYEKFSEAVSAATDEERLIKIHDVIQQLPPPHYRTLEFLMRHLSHLATFSYVTNMHTKNLAIVWAPNLLRSKQIESACFSGTAAFMEVRIQSVVVEFILNHVDVLFSPKLSSLIREGTGHNSLSRPKSLLVPSPSTKLLTLEEAQARTQAQINSPITADSKYIEVGEGPAALQGKFHTVIEFPTERKRQPMKSKKSPVGSWRSFFNLGKSSSMSKRKLQRNPSEPNELKAMALAGGRGDTGTLRSAKSEESLTSLHNVEGESKVYRPRRPRSSSDALSASFNGDLLDTRQHCNSYDNLGHGDSDGDDGPICVPALISPPRSADDVDLSPPDIGMASLDFDPMSFQCSLPLAETSIFSIDADTSSLKRSPASGSESVSPVRAKVTSRFLSPDHSTALKDKHKLLLTSFSEKTPVQSVESTEMSAVVSPPSISESAASVIMKGPESTVLQPSSPPSPLDSPTKLSSLSRTTDLPLSEAIQQELLSKAATFESKDISSIECSQPPQVTCSQEQPGAVALDSPKGNVPVSSVSLVPPPPPPKNAARMLALALAESAQQATILSQKQSSGPPTPVSPLRPQELLETLDWPLPSSLPSQPSEEETAVESSNKSVPLSSPPSTPPEKMTRSVSLHLTTADSGKPSSISCNSQDVISPETPSQSAMPSCLTSHVSQTQKSPEKQQPAVGQTPVSTAKTTTTTTTSTISSKDAVIQQPTSEIHMPETVLPPKPNKVPDQPVAILQPQPQMQTQPHTAFRSQPQVQPLPQSQAQPQSQPRSQPNARVAPTLAAPHDPVEKPWEAIKPVHPKGDSASQQHHTQGVTPPTPPIRSIESKLAAAALCNTEAANPANFHAMLETSMRGSVEETPPQSHPPHRRSSTHQSGYLYHTKGEAALLDPTTEAYYHQRVTPSGQATVGYHYRPESVPPHISYVSKSEPHISYRGHGDGRYNTIGPRSYHQSIKSRGSLRSEYISPSSLHHSHSYSQADGPTVYPTIRRVHSLHVPPTAIRSMPVTRTEVPPDDELFYYQRPIYHCKSHQPSSQVDYHVTQLQPYFENGRVQYRYSPYSGSHVLESPFYDVDHYGTIRLRHVHSFSGRDSAPLLRSGAKSGGYHYLSRHVIPLKEHGFISRDMPPYSGSKGSIYFAWDPEEAERLRIHSIRRESRARQKVKGSVMSQYDNVGPYMPVDVDMLHLRSKSDPGKTVLVSSESKEARYNVVPGSQHAPRHLISDPDVLMYMETEKHCQGNGMGEKTTKQGSSRTYSSIHSHQSQLPPLSRQHLPEGGHHDPKFEPGEKQLSGKHWQEHSDSRSAQARYERSDLDRHICRVKATSSASEDEQAAPVKPAPPPKPERSHSVRERHHYNQSSLPAYLPDNSDRDHSGTYSHQSQGQRQSTVTLQSHYDNLDDYHPVPQSQTTLANRGCSSSYHSPGFSTPHSNRAYSTALGQGAFIQAELSVQRPETEINAE

Target ORF information:

RefSeq Version	XM_009295415.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Rho GTPase activating protein 32b (arhgap32b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295415.3

ORF Insert Sequence:

ATGGAGGCTG GCAGTGGGGC TGCTGCAGTG GTGGGAAGTG CAGCTCTTGG ACTGCTCGGA 
GCCACAGGAA GTCATGATAT CTCCGACAGT CTCTTTTCTC CCCTCAGGGG CCTGAGGCCT 
GGACGGCACC CTGAAGAAGA CGATATCGTG CCAGAGCTTG CCAGGAGCAT CCACCCCCGA 
GAGCGACCTG ACTGGGAGGA AACCATCAGC GCAATGGCAC GAAGTGCTGA AATTCCAGAG 
CTGAGCTCTG AGCCGCTCAT GCGCTCCTGC AGCAGCACCG CCAGTATGAA GGTGAAGAAT 
GTCAAAAAGC TGTCATTCAC CAAGGGCCAT TTTCCAAAGT TAGCTGAATG TGCCCACTTC 
CACTATGAAA ACGTTGATTT TGGAACAATA CAGTTAACCC TCGCAGATGA GCAGACTGAA 
GTGACACGGA ATGGCCTGGA GTCAAAAGAG CCTGTGTATT TGGTTCAGAT CTACTGTCAG 
GGACGAAGCT GGATCGTGAG ACGAAGCTAT GAAGATTTCC GAGTCTTGGA TAAGCATCTC 
CATCTCTGCA TCTATGACCG CCGATTTTCC CAGCTCCCTG AGCTGCCCCG TTTCGACAGC 
CTGAGGGAGA GAGCGGAGGC AGTTTCCCAG ATGCTGATGG CTTACCTCTC CCGTCTCTCA 
GCCATTGCTG ATAACAAGAT CAATTGTGGG CCGGCCCTCA CGTGGATGGA GGTTGACAAC 
AAAGGGAATC ATTTGCTGGT GCATGAAGAA TCATCTATAA ATGTTCCAGC TATCGCAGCG 
GCCCACGTGA TCAAGCGCTA CATCGCACAG GCAGCTGATG AGCTGTCATT TGAGGTGGGG 
GATATTGTTT CTGTCATTGA CATGCCACCC AAAGAGGACA CCACCTGGTG GAGGGGAAAA 
CATGGTTTCC AGGTTGGCTT CTTTCCCAGC GAGTGTGTGG AGCTGATAAA CGACAAGGTC 
CCGCAGTCTG TCACCAACTC TGTGCCAAAA CCAGTATCCA AGAAACACGG GAAACTGATC 
ACTTTTCTGC GCACATTCAT GAAGTCCAGG CCGACCAAGC AGAAGCTGAA GCAAAGAGGT 
ATCCTCAGGG AGAGGGTGTT CGGCTGTGAC CTAGGAGAGC ATCTCCTCAA CTCTGGCCAT 
GATGTCCCCC AGGTACTCAA GAGCTGCACC GAGTTCATTG AGAAGCATGG AGTGGTTGAT 
GGAATGTATC GTCTCTCTGG GATCGCCTCC AACATTCAGA AGCTGCGGCA CGAGTTTGAT 
TCTGAACAGA TCCCAGATCT GACAAAAGAT GTTTACATTC AGGATATACA CTGCGTGGGC 
TCCCTGTGCA AGCTCTACTT TCGAGAGCTT CCCAACCCAC TTCTCACCTA CCAGCTGTAC 
GAGAAATTCT CGGAGGCTGT GTCAGCAGCC ACAGACGAGG AGAGGCTGAT CAAAATACAT 
GACGTCATTC AGCAGCTGCC GCCTCCGCAT TATAGAACCC TTGAGTTTCT TATGAGGCAT 
CTTTCACACT TGGCAACCTT CAGCTATGTA ACGAACATGC ATACTAAAAA TTTGGCCATC 
GTTTGGGCCC CCAACTTACT GAGGTCAAAG CAAATAGAAT CTGCTTGCTT CAGTGGGACA 
GCAGCCTTCA TGGAAGTACG AATACAATCG GTTGTGGTGG AGTTCATCCT GAATCATGTG 
GATGTTCTCT TCAGTCCCAA ACTGAGCTCT CTTATTCGAG AGGGAACAGG ACACAACTCT 
TTGTCTCGGC CTAAGTCTTT GCTAGTACCG TCTCCATCCA CAAAGCTGCT AACTCTAGAA 
GAGGCACAGG CACGCACACA GGCCCAGATC AACTCACCCA TCACTGCAGA CAGCAAGTAC 
ATAGAGGTCG GAGAGGGTCC TGCAGCATTA CAAGGAAAAT TCCACACAGT CATAGAATTC 
CCTACTGAGA GGAAGAGGCA GCCCATGAAG TCTAAGAAGT CTCCAGTTGG CAGTTGGCGT 
TCTTTTTTCA ACCTGGGCAA GTCTTCTTCA ATGTCGAAAC GCAAACTACA GCGTAATCCC 
AGTGAACCAA ATGAACTCAA GGCTATGGCA CTGGCTGGAG GAAGAGGAGA CACTGGTACT 
CTTCGGTCAG CCAAAAGTGA AGAGTCTCTC ACCTCTCTAC ATAATGTTGA AGGAGAGTCT 
AAAGTGTATA GGCCCCGCAG ACCTCGCTCC AGCAGTGATG CCCTCTCTGC TTCATTCAAT 
GGTGACCTTC TGGATACCAG ACAGCACTGT AACTCGTACG ACAACCTAGG TCATGGAGAC 
AGTGATGGGG ATGATGGTCC AATCTGTGTG CCTGCTCTCA TCTCCCCACC ACGCTCCGCT 
GATGATGTGG ATCTGAGCCC CCCTGACATT GGCATGGCCT CTTTAGACTT TGACCCCATG 
TCTTTCCAAT GTAGCCTTCC TCTGGCAGAA ACTTCGATTT TCTCAATTGA CGCAGATACA 
AGCAGTCTAA AAAGGAGCCC TGCCAGTGGC TCAGAGTCAG TCTCGCCAGT CCGAGCTAAA 
GTTACGAGCC GGTTTTTGTC TCCAGACCAC AGTACAGCCT TAAAGGATAA ACATAAACTG 
CTCCTTACAT CTTTCTCTGA GAAAACTCCA GTTCAGTCTG TTGAAAGCAC CGAGATGTCA 
GCAGTTGTTT CTCCTCCAAG CATCTCTGAA TCAGCTGCCT CCGTGATAAT GAAAGGACCA 
GAGAGCACTG TGCTTCAGCC CTCTTCCCCT CCATCTCCAC TCGACTCTCC TACAAAATTG 
AGCTCTCTGT CAAGAACCAC CGATCTGCCC CTGAGTGAAG CCATTCAACA AGAGCTTCTT 
TCTAAAGCTG CTACCTTTGA GAGTAAAGAC ATATCGAGCA TTGAATGTTC ACAACCACCA 
CAGGTCACCT GCTCTCAAGA ACAGCCAGGA GCCGTAGCTT TGGACTCTCC AAAGGGCAAT 
GTCCCTGTCA GCTCTGTGTC CCTCGTCCCT CCCCCTCCCC CACCAAAGAA CGCTGCCCGC 
ATGCTTGCAC TAGCTCTAGC TGAATCTGCC CAGCAGGCCA CCATTCTTTC TCAGAAGCAA 
TCCTCTGGAC CCCCTACACC AGTATCCCCT CTTAGGCCTC AAGAGTTATT GGAGACCCTG 
GACTGGCCGC TGCCTTCTTC CTTGCCATCA CAGCCATCCG AGGAGGAAAC TGCAGTAGAA 
TCATCAAATA AATCGGTTCC TTTGTCTTCA CCACCCTCCA CTCCACCTGA AAAAATGACT 
CGCTCAGTCA GTCTGCACCT CACCACAGCT GATAGTGGGA AGCCATCCAG CATTTCATGC 
AACAGCCAGG ATGTAATCTC ACCAGAGACC CCAAGCCAGA GTGCTATGCC CTCTTGTCTG 
ACTTCTCATG TTTCTCAAAC ACAAAAAAGT CCAGAAAAAC AGCAGCCTGC TGTCGGTCAG 
ACACCTGTGA GCACAGCAAA AACCACCACC ACAACCACCA CTTCAACCAT CAGCAGTAAG 
GATGCTGTCA TTCAACAACC CACCTCTGAG ATCCATATGC CTGAAACAGT CCTGCCACCA 
AAACCAAACA AGGTGCCAGA TCAACCAGTG GCAATTCTTC AGCCTCAGCC TCAAATGCAG 
ACGCAACCTC ATACTGCATT TCGGTCTCAA CCTCAAGTTC AGCCTCTTCC TCAGTCACAA 
GCACAGCCAC AGTCTCAGCC CAGATCACAG CCCAATGCTA GAGTTGCACC AACCCTGGCA 
GCACCTCATG ATCCTGTGGA AAAACCATGG GAAGCCATAA AGCCTGTCCA TCCAAAAGGA 
GATAGTGCTT CTCAACAACA CCACACTCAG GGAGTGACAC CACCAACACC CCCTATTCGC 
TCGATTGAAA GTAAACTAGC AGCAGCTGCA CTTTGCAACA CCGAGGCTGC AAACCCTGCC 
AACTTTCATG CCATGTTAGA GACATCCATG AGGGGATCAG TAGAGGAGAC TCCCCCACAG 
TCTCATCCGC CTCACCGCAG ATCGTCTACT CATCAGTCAG GTTATCTTTA TCACACAAAA 
GGGGAGGCAG CCTTACTGGA CCCCACCACA GAGGCATATT ACCATCAGCG GGTAACCCCT 
TCAGGTCAGG CTACAGTGGG GTACCATTAC CGGCCAGAGA GTGTTCCACC TCACATTTCT 
TACGTGTCAA AATCAGAGCC ACACATTTCC TACAGAGGTC ATGGGGATGG TCGATACAAT 
ACAATTGGAC CTAGATCCTA CCACCAGTCT ATCAAATCTC GTGGTTCACT TAGAAGTGAG 
TACATCTCTC CAAGCTCATT ACACCACTCC CATAGCTACA GCCAAGCAGA TGGACCAACC 
GTCTACCCAA CAATTCGCAG GGTCCACTCC TTACATGTGC CTCCCACTGC TATTCGCTCA 
ATGCCAGTCA CCAGAACTGA GGTACCACCA GATGATGAGT TATTTTACTA CCAGCGCCCA 
ATCTACCACT GCAAGTCTCA CCAACCATCA TCTCAAGTCG ACTATCACGT CACACAACTG 
CAGCCTTATT TTGAAAATGG AAGAGTTCAG TACCGCTACA GCCCCTACTC TGGCTCTCAC 
GTTCTGGAAT CCCCTTTCTA TGATGTGGAC CATTATGGTA CTATCCGCTT AAGGCACGTC 
CATTCCTTCA GTGGTCGGGA CAGTGCCCCT CTCCTTCGAT CTGGAGCAAA ATCTGGAGGC 
TACCACTACC TTTCACGGCA TGTTATTCCT TTAAAGGAGC ATGGCTTCAT AAGCAGGGAC 
ATGCCACCTT ACAGTGGTTC AAAAGGGAGT ATTTACTTTG CATGGGACCC AGAGGAGGCA 
GAAAGGCTGC GCATACACTC CATCCGTAGG GAAAGCAGAG CAAGGCAAAA GGTTAAGGGT 
TCTGTTATGT CTCAATACGA CAATGTAGGA CCCTACATGC CTGTGGATGT TGACATGTTA 
CATTTGCGCA GCAAATCAGA TCCTGGAAAA ACTGTATTGG TGTCCTCGGA GAGCAAAGAG 
GCCAGATACA ATGTTGTGCC TGGATCTCAG CATGCTCCCA GACACTTGAT CTCTGATCCA 
GATGTCCTAA TGTATATGGA AACAGAGAAG CACTGCCAAG GAAATGGAAT GGGGGAGAAA 
ACCACCAAGC AAGGCAGCTC TAGGACCTAC TCGTCTATAC ATTCACATCA ATCCCAGCTT 
CCACCACTAA GCCGGCAGCA CTTACCAGAG GGTGGCCATC ATGACCCAAA GTTTGAACCA 
GGGGAAAAGC AACTGAGTGG TAAACATTGG CAGGAACATT CGGACAGCAG GAGTGCTCAA 
GCACGTTATG AAAGGTCTGA TTTGGATCGC CACATATGCA GGGTGAAGGC CACTAGCAGT 
GCCAGTGAAG ATGAACAAGC TGCTCCTGTG AAGCCGGCTC CTCCACCTAA GCCCGAGAGA 
TCCCATAGTG TTCGAGAGCG GCACCATTAC AACCAGTCCA GTCTTCCTGC ATATTTACCA 
GACAATTCAG ATAGAGACCA CAGTGGAACA TACTCCCATC AGTCACAGGG GCAGAGACAA 
AGCACCGTTA CTTTGCAGTC GCATTACGAC AACCTGGATG ACTATCATCC AGTACCTCAA 
TCCCAAACCA CGCTAGCAAA TCGGGGTTGT TCCAGTTCCT ATCATAGCCC CGGTTTCTCA 
ACTCCACACA GTAATCGTGC ATACTCCACT GCCTTGGGAC AGGGTGCCTT CATACAAGCT 
GAGCTTTCAG TGCAAAGGCC AGAGACTGAG ATAAATGCTG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	arhgap32b
Entrez Gene ID	569434
Full Name	Rho GTPase activating protein 32b
Synonyms	im:7150060,si:dkey-269g4.4
General protein information	Preferred Names Rho GTPase activating protein 32b Names rho GTPase-activating protein 32
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Bos taurus (cattle)	ARHGAP32	NP_001179016.1
Danio rerio (zebrafish)	im:7150060	XP_005161318.1
Pan troglodytes (chimpanzee)	ARHGAP32	XP_003313461.2
Canis lupus familiaris (dog)	ARHGAP32	XP_546401.3
Mus musculus (house mouse)	Arhgap32	NP_001182561.1
Xenopus tropicalis (tropical clawed frog)	arhgap32	XP_004916220.1
Homo sapiens (human)	ARHGAP32	NP_001136157.1
Rattus norvegicus (Norway rat)	Arhgap32	XP_006242835.1
	ARHGAP32	XP_001111190.2
Gallus gallus (chicken)	ARHGAP32	XP_004948019.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

arhgap32b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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