gja8a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	gja8a
Entrez Gene ID	569409
Full Name	gap junction protein alpha 8 paralog a
Synonyms	cx50.5,cx79.8,si:dkey-18p12.7
General protein information	Preferred Names gap junction protein alpha 8 paralog a Names gap junction protein alpha 8 paralog a 44.1 protein-like connexin 50.5
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005167743.3	XP_005167800.1	connexin 50.5 isoform X1
NM_001128350.1	NP_001121822.1	gap junction protein alpha 8 paralog a

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Zebrafish connexin 79.8 (Gja8a): A lens connexin used as an electrical synapse in some neurons.
Yoshikawa S, Vila A, Segelken J, Lin YP, Mitchell CK, Nguyen D, O'Brien J
Developmental neurobiology77(5)548-561(2017 05)

Complexity of gap junctions between horizontal cells of the carp retina.
Greb H, Hermann S, Dirks P, Ommen G, Kretschmer V, Schultz K, Zoidl G, Weiler R, Janssen-Bienhold U
Neuroscience3408-22(2017 Jan)

The Physiological Characterization of Connexin41.8 and Connexin39.4, Which Are Involved in the Striped Pattern Formation of Zebrafish.
Watanabe M, Sawada R, Aramaki T, Skerrett IM, Kondo S
The Journal of biological chemistry291(3)1053-63(2016 Jan)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Cx23, a connexin with only four extracellular-loop cysteines, forms functional gap junction channels and hemichannels.
Iovine MK, Gumpert AM, Falk MM, Mendelson TC
FEBS letters582(2)165-70(2008 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following gja8a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gja8a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa04371	NM_001128350.1 Latest version!	Danio rerio gap junction protein alpha 8 paralog a (gja8a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa04371	XM_005167743.3 Latest version!	Danio rerio connexin 50.5 (cx50.5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

gja8a ( NM_001128350.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001121822.1 Danio rerio gap junction protein alpha 8 paralog a (gja8a), mRNA.

Price & Availability↑

CloneID	ODa04371
Clone ID Related Accession (Same CDS sequence)	XM_005167743.3 , NM_001128350.1
Accession Version	NM_001128350.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-07
Organism	Danio rerio(zebrafish)
Product	gap junction protein alpha 8 paralog a
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX571845.6. On Jul 31, 2008 this sequence version replaced XM_692792.2.

ATGGGAGACT GGAGCTTCTT GGGTAATATT TTGGAGGAAG TAAATGAACA TTCAACTGTG 
ATTGGTCGTG TTTGGCTCAC AGTACTATTC ATTTTCCGAA TTTTAATTCT GGGCACGGCT 
GCTGAGTTTG TCTGGGGAGA CGAGCAGTCC GATTACGTGT GCAACACTCA GCAGCCAGGA 
TGCGAGAATG TGTGCTATGA TGAGGCCTTT CCAATCTCTC ACATTCGCCT ATGGGTGTTA 
CAGATCATCT TTGTATCCAC ACCTTCACTT GTATACGTGG GCCATGCTGT CCATTACATC 
CACATGGAGG AGAAACGAAA GGAGCGGGAA GAAGCTGAGG TCAGCCACCA GCAAGAACTT 
TGCGAGGAGC GCCAGGCAAT GGATCAAGGA AGCGTTCGCA CTGCCAAGGA GACCAGCACG 
AAGGGGAGCA AGAAGTTTAG ACTTGAAGGA ACCCTGCTGT GCACCTACAT CTGCCATATC 
ATCTTCAAAG CTCTGTTTGA AATAGGCTTT GTAGTGGGAC AATATTTCCT TTATGGGTTC 
CGCATCCTGC CTTTGTACAA GTGCAGTCGT TGGCCATGCC CTAACACAGT GGACTGCTAC 
GTCTCCCGGC CCACCGAAAA GACCATCTTC ATCATTTTCA TGCTTGCAGT GGCTTGTGTT 
TCACTGTTCC TCAACTTTGT GGAGATTAGC CACCTCGGCT TGAAGAAGAT CCGCTTTGTG 
TTCCACCGAC CAGCTCCAGC ACAGTTAGAG TCGCTTGGAC CTCCTGAGAG GAGTTTACCG 
TTTCTTCTAA CCACCCCTGT CCAAAAAGCC AAAGGCTACA GGCGCCTCGA AGAGGAGAAG 
AAAGACGAGG TGGCTCATAT CTATCCACTA GCTGAGGTTG GGATGGAAGA GGGACAGTTC 
TTCTTACCTC AGCTGGAGAA GGAGCAGAAA AGCAGTCAGG AGGCAATTCT GCCAACAGCG 
CCACCTGTAG AGGAGACAAT TATATGCGAT GAGACTCAAC CCTCCTTCCT TCAGGTCACA 
GAGACATTAC CAGAGCTCCC AACTGAAGAG CCGCCTAGGG AAGGAGATGA GGTAGACAGT 
CTAAAAACTC CAACAGTGCT CCCAGAAGTA CTAGAGGAGC ATTCAGAAGG GGAAAGCGTG 
GAGGAAACAT ATTTAATTTC ACTTGAGGAG AATTTGGATG TAGATGTAGG GAAAGTGGTG 
ACTGAAGAAA AGTTGCTAAG AGAGAGTAGC TTAGTAGATA CTGAGCCAAA ACAAGAGGAA 
AACCTTTCAG GAGATGGAGA GGAGAAGGAA ACCTCACAGG AAATTGGGGA AACAGAGGAT 
AGCAACTTTC CAACCAAAAG GCTGAAGGAG GAAGAGGGTT TACCTGATGT GGTTGAAGAA 
GCATCTGATG AAGAGAGAGA ACTTTGTGAG CTTGAGCCTT TTGTGAATGA GGACACTGTG 
GAGGAGGTGA AAACTTTAGA TGATGTGAAT CCTGACGATC TGGATATGTC CAAAATATCT 
GAAGGAGAAC AAGAGTCTGA GGCTTTAGCG GAAAATGTAG CATCAAATCT TCCTGACATA 
GCGCCTATTG GGGATGAAGT GGATATGGGA AGGGACAAAG CTCAGAGTGA TGTAGATGAT 
AAAGAAGATG GAGTTCAAAG TGATGTAGTA GACTCAGGAG TTTTAGAAGA TTTAGTGGAG 
ATTAAAAAGG TCAGAGCTCT AGATGATCTA CAAGATGCAG AAGATTGTTG TCTAGATATA 
GATAAGAACG ATCCTTTAGA AAATGAGGAT ATACCTCTTG ACACTGCAGT CAATACAAGT 
GGAGAGGATC TAGATAAACC TCTAGGACTT TCAGGTGACA CTAACCAGTT GAAAACTTTG 
AGGAAGGAAG AGGTATCATT GGGGATAGAA GATCCACAGG AGGTCAAAGG CTCTGAGTTG 
GAAGAGTCAA GACAAGAAGA TGAGTCTGTG AAAACTGGAG ACCTTGAGAA GGAAGAATAT 
TCTGAAGAAT CAAAAGCCTT GGAGGCTTTA GAGGAGATGA TAGATGCTCC ACTTGTAGCT 
CTAGATTTAG AACCAACAGA GGAAACAAGA TCTTCAAGTC GTCTCAGCAA AGGTAGCAGC 
AGAGCCAGGT CAGATGATCT AACTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121822.1
CDS	1..2130
Translation	MGDWSFLGNILEEVNEHSTVIGRVWLTVLFIFRILILGTAAEFVWGDEQSDYVCNTQQPGCENVCYDEAFPISHIRLWVLQIIFVSTPSLVYVGHAVHYIHMEEKRKEREEAEVSHQQELCEERQAMDQGSVRTAKETSTKGSKKFRLEGTLLCTYICHIIFKALFEIGFVVGQYFLYGFRILPLYKCSRWPCPNTVDCYVSRPTEKTIFIIFMLAVACVSLFLNFVEISHLGLKKIRFVFHRPAPAQLESLGPPERSLPFLLTTPVQKAKGYRRLEEEKKDEVAHIYPLAEVGMEEGQFFLPQLEKEQKSSQEAILPTAPPVEETIICDETQPSFLQVTETLPELPTEEPPREGDEVDSLKTPTVLPEVLEEHSEGESVEETYLISLEENLDVDVGKVVTEEKLLRESSLVDTEPKQEENLSGDGEEKETSQEIGETEDSNFPTKRLKEEEGLPDVVEEASDEERELCELEPFVNEDTVEEVKTLDDVNPDDLDMSKISEGEQESEALAENVASNLPDIAPIGDEVDMGRDKAQSDVDDKEDGVQSDVVDSGVLEDLVEIKKVRALDDLQDAEDCCLDIDKNDPLENEDIPLDTAVNTSGEDLDKPLGLSGDTNQLKTLRKEEVSLGIEDPQEVKGSELEESRQEDESVKTGDLEKEEYSEESKALEALEEMIDAPLVALDLEPTEETRSSSRLSKGSSRARSDDLTI

Target ORF information:

RefSeq Version	NM_001128350.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio gap junction protein alpha 8 paralog a (gja8a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128350.1

ORF Insert Sequence:

ATGGGAGACT GGAGCTTCTT GGGTAATATT TTGGAGGAAG TAAATGAACA TTCAACTGTG 
ATTGGTCGTG TTTGGCTCAC AGTACTATTC ATTTTCCGAA TTTTAATTCT GGGCACGGCT 
GCTGAGTTTG TCTGGGGAGA CGAGCAGTCC GATTACGTGT GCAACACTCA GCAGCCAGGA 
TGCGAGAATG TGTGCTATGA TGAGGCCTTT CCAATCTCTC ACATTCGCCT ATGGGTGTTA 
CAGATCATCT TTGTATCCAC ACCTTCACTT GTATACGTGG GCCATGCTGT CCATTACATC 
CACATGGAGG AGAAACGAAA GGAGCGGGAA GAAGCTGAGG TCAGCCACCA GCAAGAACTT 
TGCGAGGAGC GCCAGGCAAT GGATCAAGGA AGCGTTCGCA CTGCCAAGGA GACCAGCACG 
AAGGGGAGCA AGAAGTTTAG ACTTGAAGGA ACCCTGCTGT GCACCTACAT CTGCCATATC 
ATCTTCAAAG CTCTGTTTGA AATAGGCTTT GTAGTGGGAC AATATTTCCT TTATGGGTTC 
CGCATCCTGC CTTTGTACAA GTGCAGTCGT TGGCCATGCC CTAACACAGT GGACTGCTAC 
GTCTCCCGGC CCACCGAAAA GACCATCTTC ATCATTTTCA TGCTTGCAGT GGCTTGTGTT 
TCACTGTTCC TCAACTTTGT GGAGATTAGC CACCTCGGCT TGAAGAAGAT CCGCTTTGTG 
TTCCACCGAC CAGCTCCAGC ACAGTTAGAG TCGCTTGGAC CTCCTGAGAG GAGTTTACCG 
TTTCTTCTAA CCACCCCTGT CCAAAAAGCC AAAGGCTACA GGCGCCTCGA AGAGGAGAAG 
AAAGACGAGG TGGCTCATAT CTATCCACTA GCTGAGGTTG GGATGGAAGA GGGACAGTTC 
TTCTTACCTC AGCTGGAGAA GGAGCAGAAA AGCAGTCAGG AGGCAATTCT GCCAACAGCG 
CCACCTGTAG AGGAGACAAT TATATGCGAT GAGACTCAAC CCTCCTTCCT TCAGGTCACA 
GAGACATTAC CAGAGCTCCC AACTGAAGAG CCGCCTAGGG AAGGAGATGA GGTAGACAGT 
CTAAAAACTC CAACAGTGCT CCCAGAAGTA CTAGAGGAGC ATTCAGAAGG GGAAAGCGTG 
GAGGAAACAT ATTTAATTTC ACTTGAGGAG AATTTGGATG TAGATGTAGG GAAAGTGGTG 
ACTGAAGAAA AGTTGCTAAG AGAGAGTAGC TTAGTAGATA CTGAGCCAAA ACAAGAGGAA 
AACCTTTCAG GAGATGGAGA GGAGAAGGAA ACCTCACAGG AAATTGGGGA AACAGAGGAT 
AGCAACTTTC CAACCAAAAG GCTGAAGGAG GAAGAGGGTT TACCTGATGT GGTTGAAGAA 
GCATCTGATG AAGAGAGAGA ACTTTGTGAG CTTGAGCCTT TTGTGAATGA GGACACTGTG 
GAGGAGGTGA AAACTTTAGA TGATGTGAAT CCTGACGATC TGGATATGTC CAAAATATCT 
GAAGGAGAAC AAGAGTCTGA GGCTTTAGCG GAAAATGTAG CATCAAATCT TCCTGACATA 
GCGCCTATTG GGGATGAAGT GGATATGGGA AGGGACAAAG CTCAGAGTGA TGTAGATGAT 
AAAGAAGATG GAGTTCAAAG TGATGTAGTA GACTCAGGAG TTTTAGAAGA TTTAGTGGAG 
ATTAAAAAGG TCAGAGCTCT AGATGATCTA CAAGATGCAG AAGATTGTTG TCTAGATATA 
GATAAGAACG ATCCTTTAGA AAATGAGGAT ATACCTCTTG ACACTGCAGT CAATACAAGT 
GGAGAGGATC TAGATAAACC TCTAGGACTT TCAGGTGACA CTAACCAGTT GAAAACTTTG 
AGGAAGGAAG AGGTATCATT GGGGATAGAA GATCCACAGG AGGTCAAAGG CTCTGAGTTG 
GAAGAGTCAA GACAAGAAGA TGAGTCTGTG AAAACTGGAG ACCTTGAGAA GGAAGAATAT 
TCTGAAGAAT CAAAAGCCTT GGAGGCTTTA GAGGAGATGA TAGATGCTCC ACTTGTAGCT 
CTAGATTTAG AACCAACAGA GGAAACAAGA TCTTCAAGTC GTCTCAGCAA AGGTAGCAGC 
AGAGCCAGGT CAGATGATCT AACTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

gja8a ( XM_005167743.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167800.1 Danio rerio connexin 50.5 (cx50.5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa04371
Clone ID Related Accession (Same CDS sequence)	XM_005167743.3 , NM_001128350.1
Accession Version	XM_005167743.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	connexin 50.5 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167743.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGACT GGAGCTTCTT GGGTAATATT TTGGAGGAAG TAAATGAACA TTCAACTGTG 
ATTGGTCGTG TTTGGCTCAC AGTACTATTC ATTTTCCGAA TTTTAATTCT GGGCACGGCT 
GCTGAGTTTG TCTGGGGAGA CGAGCAGTCC GATTACGTGT GCAACACTCA GCAGCCAGGA 
TGCGAGAATG TGTGCTATGA TGAGGCCTTT CCAATCTCTC ACATTCGCCT ATGGGTGTTA 
CAGATCATCT TTGTATCCAC ACCTTCACTT GTATACGTGG GCCATGCTGT CCATTACATC 
CACATGGAGG AGAAACGAAA GGAGCGGGAA GAAGCTGAGG TCAGCCACCA GCAAGAACTT 
TGCGAGGAGC GCCAGGCAAT GGATCAAGGA AGCGTTCGCA CTGCCAAGGA GACCAGCACG 
AAGGGGAGCA AGAAGTTTAG ACTTGAAGGA ACCCTGCTGT GCACCTACAT CTGCCATATC 
ATCTTCAAAG CTCTGTTTGA AATAGGCTTT GTAGTGGGAC AATATTTCCT TTATGGGTTC 
CGCATCCTGC CTTTGTACAA GTGCAGTCGT TGGCCATGCC CTAACACAGT GGACTGCTAC 
GTCTCCCGGC CCACCGAAAA GACCATCTTC ATCATTTTCA TGCTTGCAGT GGCTTGTGTT 
TCACTGTTCC TCAACTTTGT GGAGATTAGC CACCTCGGCT TGAAGAAGAT CCGCTTTGTG 
TTCCACCGAC CAGCTCCAGC ACAGTTAGAG TCGCTTGGAC CTCCTGAGAG GAGTTTACCG 
TTTCTTCTAA CCACCCCTGT CCAAAAAGCC AAAGGCTACA GGCGCCTCGA AGAGGAGAAG 
AAAGACGAGG TGGCTCATAT CTATCCACTA GCTGAGGTTG GGATGGAAGA GGGACAGTTC 
TTCTTACCTC AGCTGGAGAA GGAGCAGAAA AGCAGTCAGG AGGCAATTCT GCCAACAGCG 
CCACCTGTAG AGGAGACAAT TATATGCGAT GAGACTCAAC CCTCCTTCCT TCAGGTCACA 
GAGACATTAC CAGAGCTCCC AACTGAAGAG CCGCCTAGGG AAGGAGATGA GGTAGACAGT 
CTAAAAACTC CAACAGTGCT CCCAGAAGTA CTAGAGGAGC ATTCAGAAGG GGAAAGCGTG 
GAGGAAACAT ATTTAATTTC ACTTGAGGAG AATTTGGATG TAGATGTAGG GAAAGTGGTG 
ACTGAAGAAA AGTTGCTAAG AGAGAGTAGC TTAGTAGATA CTGAGCCAAA ACAAGAGGAA 
AACCTTTCAG GAGATGGAGA GGAGAAGGAA ACCTCACAGG AAATTGGGGA AACAGAGGAT 
AGCAACTTTC CAACCAAAAG GCTGAAGGAG GAAGAGGGTT TACCTGATGT GGTTGAAGAA 
GCATCTGATG AAGAGAGAGA ACTTTGTGAG CTTGAGCCTT TTGTGAATGA GGACACTGTG 
GAGGAGGTGA AAACTTTAGA TGATGTGAAT CCTGACGATC TGGATATGTC CAAAATATCT 
GAAGGAGAAC AAGAGTCTGA GGCTTTAGCG GAAAATGTAG CATCAAATCT TCCTGACATA 
GCGCCTATTG GGGATGAAGT GGATATGGGA AGGGACAAAG CTCAGAGTGA TGTAGATGAT 
AAAGAAGATG GAGTTCAAAG TGATGTAGTA GACTCAGGAG TTTTAGAAGA TTTAGTGGAG 
ATTAAAAAGG TCAGAGCTCT AGATGATCTA CAAGATGCAG AAGATTGTTG TCTAGATATA 
GATAAGAACG ATCCTTTAGA AAATGAGGAT ATACCTCTTG ACACTGCAGT CAATACAAGT 
GGAGAGGATC TAGATAAACC TCTAGGACTT TCAGGTGACA CTAACCAGTT GAAAACTTTG 
AGGAAGGAAG AGGTATCATT GGGGATAGAA GATCCACAGG AGGTCAAAGG CTCTGAGTTG 
GAAGAGTCAA GACAAGAAGA TGAGTCTGTG AAAACTGGAG ACCTTGAGAA GGAAGAATAT 
TCTGAAGAAT CAAAAGCCTT GGAGGCTTTA GAGGAGATGA TAGATGCTCC ACTTGTAGCT 
CTAGATTTAG AACCAACAGA GGAAACAAGA TCTTCAAGTC GTCTCAGCAA AGGTAGCAGC 
AGAGCCAGGT CAGATGATCT AACTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167800.1
CDS	918..3047
Translation	MGDWSFLGNILEEVNEHSTVIGRVWLTVLFIFRILILGTAAEFVWGDEQSDYVCNTQQPGCENVCYDEAFPISHIRLWVLQIIFVSTPSLVYVGHAVHYIHMEEKRKEREEAEVSHQQELCEERQAMDQGSVRTAKETSTKGSKKFRLEGTLLCTYICHIIFKALFEIGFVVGQYFLYGFRILPLYKCSRWPCPNTVDCYVSRPTEKTIFIIFMLAVACVSLFLNFVEISHLGLKKIRFVFHRPAPAQLESLGPPERSLPFLLTTPVQKAKGYRRLEEEKKDEVAHIYPLAEVGMEEGQFFLPQLEKEQKSSQEAILPTAPPVEETIICDETQPSFLQVTETLPELPTEEPPREGDEVDSLKTPTVLPEVLEEHSEGESVEETYLISLEENLDVDVGKVVTEEKLLRESSLVDTEPKQEENLSGDGEEKETSQEIGETEDSNFPTKRLKEEEGLPDVVEEASDEERELCELEPFVNEDTVEEVKTLDDVNPDDLDMSKISEGEQESEALAENVASNLPDIAPIGDEVDMGRDKAQSDVDDKEDGVQSDVVDSGVLEDLVEIKKVRALDDLQDAEDCCLDIDKNDPLENEDIPLDTAVNTSGEDLDKPLGLSGDTNQLKTLRKEEVSLGIEDPQEVKGSELEESRQEDESVKTGDLEKEEYSEESKALEALEEMIDAPLVALDLEPTEETRSSSRLSKGSSRARSDDLTI

Target ORF information:

RefSeq Version	XM_005167743.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio connexin 50.5 (cx50.5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167743.3

ORF Insert Sequence:

ATGGGAGACT GGAGCTTCTT GGGTAATATT TTGGAGGAAG TAAATGAACA TTCAACTGTG 
ATTGGTCGTG TTTGGCTCAC AGTACTATTC ATTTTCCGAA TTTTAATTCT GGGCACGGCT 
GCTGAGTTTG TCTGGGGAGA CGAGCAGTCC GATTACGTGT GCAACACTCA GCAGCCAGGA 
TGCGAGAATG TGTGCTATGA TGAGGCCTTT CCAATCTCTC ACATTCGCCT ATGGGTGTTA 
CAGATCATCT TTGTATCCAC ACCTTCACTT GTATACGTGG GCCATGCTGT CCATTACATC 
CACATGGAGG AGAAACGAAA GGAGCGGGAA GAAGCTGAGG TCAGCCACCA GCAAGAACTT 
TGCGAGGAGC GCCAGGCAAT GGATCAAGGA AGCGTTCGCA CTGCCAAGGA GACCAGCACG 
AAGGGGAGCA AGAAGTTTAG ACTTGAAGGA ACCCTGCTGT GCACCTACAT CTGCCATATC 
ATCTTCAAAG CTCTGTTTGA AATAGGCTTT GTAGTGGGAC AATATTTCCT TTATGGGTTC 
CGCATCCTGC CTTTGTACAA GTGCAGTCGT TGGCCATGCC CTAACACAGT GGACTGCTAC 
GTCTCCCGGC CCACCGAAAA GACCATCTTC ATCATTTTCA TGCTTGCAGT GGCTTGTGTT 
TCACTGTTCC TCAACTTTGT GGAGATTAGC CACCTCGGCT TGAAGAAGAT CCGCTTTGTG 
TTCCACCGAC CAGCTCCAGC ACAGTTAGAG TCGCTTGGAC CTCCTGAGAG GAGTTTACCG 
TTTCTTCTAA CCACCCCTGT CCAAAAAGCC AAAGGCTACA GGCGCCTCGA AGAGGAGAAG 
AAAGACGAGG TGGCTCATAT CTATCCACTA GCTGAGGTTG GGATGGAAGA GGGACAGTTC 
TTCTTACCTC AGCTGGAGAA GGAGCAGAAA AGCAGTCAGG AGGCAATTCT GCCAACAGCG 
CCACCTGTAG AGGAGACAAT TATATGCGAT GAGACTCAAC CCTCCTTCCT TCAGGTCACA 
GAGACATTAC CAGAGCTCCC AACTGAAGAG CCGCCTAGGG AAGGAGATGA GGTAGACAGT 
CTAAAAACTC CAACAGTGCT CCCAGAAGTA CTAGAGGAGC ATTCAGAAGG GGAAAGCGTG 
GAGGAAACAT ATTTAATTTC ACTTGAGGAG AATTTGGATG TAGATGTAGG GAAAGTGGTG 
ACTGAAGAAA AGTTGCTAAG AGAGAGTAGC TTAGTAGATA CTGAGCCAAA ACAAGAGGAA 
AACCTTTCAG GAGATGGAGA GGAGAAGGAA ACCTCACAGG AAATTGGGGA AACAGAGGAT 
AGCAACTTTC CAACCAAAAG GCTGAAGGAG GAAGAGGGTT TACCTGATGT GGTTGAAGAA 
GCATCTGATG AAGAGAGAGA ACTTTGTGAG CTTGAGCCTT TTGTGAATGA GGACACTGTG 
GAGGAGGTGA AAACTTTAGA TGATGTGAAT CCTGACGATC TGGATATGTC CAAAATATCT 
GAAGGAGAAC AAGAGTCTGA GGCTTTAGCG GAAAATGTAG CATCAAATCT TCCTGACATA 
GCGCCTATTG GGGATGAAGT GGATATGGGA AGGGACAAAG CTCAGAGTGA TGTAGATGAT 
AAAGAAGATG GAGTTCAAAG TGATGTAGTA GACTCAGGAG TTTTAGAAGA TTTAGTGGAG 
ATTAAAAAGG TCAGAGCTCT AGATGATCTA CAAGATGCAG AAGATTGTTG TCTAGATATA 
GATAAGAACG ATCCTTTAGA AAATGAGGAT ATACCTCTTG ACACTGCAGT CAATACAAGT 
GGAGAGGATC TAGATAAACC TCTAGGACTT TCAGGTGACA CTAACCAGTT GAAAACTTTG 
AGGAAGGAAG AGGTATCATT GGGGATAGAA GATCCACAGG AGGTCAAAGG CTCTGAGTTG 
GAAGAGTCAA GACAAGAAGA TGAGTCTGTG AAAACTGGAG ACCTTGAGAA GGAAGAATAT 
TCTGAAGAAT CAAAAGCCTT GGAGGCTTTA GAGGAGATGA TAGATGCTCC ACTTGTAGCT 
CTAGATTTAG AACCAACAGA GGAAACAAGA TCTTCAAGTC GTCTCAGCAA AGGTAGCAGC 
AGAGCCAGGT CAGATGATCT AACTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Zebrafish connexin 79.8 (Gja8a): A lens connexin used as an electrical synapse in some neurons.
Developmental neurobiology77(5)548-561(2017 05)
Yoshikawa S,Vila A,Segelken J,Lin YP,Mitchell CK,Nguyen D,O'Brien J

Complexity of gap junctions between horizontal cells of the carp retina.
Neuroscience3408-22(2017 Jan)
Greb H,Hermann S,Dirks P,Ommen G,Kretschmer V,Schultz K,Zoidl G,Weiler R,Janssen-Bienhold U

The Physiological Characterization of Connexin41.8 and Connexin39.4, Which Are Involved in the Striped Pattern Formation of Zebrafish.
The Journal of biological chemistry291(3)1053-63(2016 Jan)
Watanabe M,Sawada R,Aramaki T,Skerrett IM,Kondo S

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Cx23, a connexin with only four extracellular-loop cysteines, forms functional gap junction channels and hemichannels.
FEBS letters582(2)165-70(2008 Jan)
Iovine MK,Gumpert AM,Falk MM,Mendelson TC

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gja8a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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