kdm6a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	kdm6a
Entrez Gene ID	569277
Full Name	lysine (K)-specific demethylase 6A
Synonyms	si:ch211-222m21.1,utx
General protein information	Preferred Names lysine (K)-specific demethylase 6A Names lysine-specific demethylase 6A ubiquitously transcribed tetratricopeptide repeat, X chromosome
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017357834.1	XP_017213323.1	lysine-specific demethylase 6A isoform X3
XM_005167798.3	XP_005167855.1	lysine-specific demethylase 6A isoform X4
XM_005167795.3	XP_005167852.1	lysine-specific demethylase 6A isoform X2
XM_005167794.4	XP_005167851.1	lysine-specific demethylase 6A isoform X1
XM_005167795.4	XP_005167852.1	lysine-specific demethylase 6A isoform X2
XM_005167794.3	XP_005167851.1	lysine-specific demethylase 6A isoform X1
XM_017357834.2	XP_017213323.1	lysine-specific demethylase 6A isoform X3
XM_005167798.4	XP_005167855.1	lysine-specific demethylase 6A isoform X4

Pathways from BioSystems

Homologies

Homo sapiens (human)	KDM6A	NP_066963.2
Danio rerio (zebrafish)	kdm6a	XP_697746.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008509	XP_316935.4
Xenopus tropicalis (tropical clawed frog)	kdm6a	NP_001123807.1
Bos taurus (cattle)	KDM6A	NP_001193504.1
Drosophila melanogaster (fruit fly)	Utx	NP_001188773.1
Pan troglodytes (chimpanzee)	KDM6A	XP_003954022.1
Canis lupus familiaris (dog)	KDM6A	NP_001184114.1
Caenorhabditis elegans (roundworm)	utx-1	NP_509450.1
Rattus norvegicus (Norway rat)	Kdm6a	XP_006256701.1
	KDM6A	XP_002806253.1
Mus musculus (house mouse)	Kdm6a	NP_033509.1
Gallus gallus (chicken)	KDM6A	XP_416762.4

Gene Ontology

The following kdm6a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kdm6a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa60364	XM_017357834.2 Latest version!	Danio rerio lysine (K)-specific demethylase 6A (kdm6a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa15362	XM_005167798.4 Latest version!	Danio rerio lysine (K)-specific demethylase 6A (kdm6a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa15357	XM_005167794.4 Latest version!	Danio rerio lysine (K)-specific demethylase 6A (kdm6a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa15358	XM_005167795.4 Latest version!	Danio rerio lysine (K)-specific demethylase 6A (kdm6a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa15358	XM_005167795.3	Danio rerio lysine (K)-specific demethylase 6A (kdm6a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa15357	XM_005167794.3	Danio rerio lysine (K)-specific demethylase 6A (kdm6a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa60364	XM_017357834.1	Danio rerio lysine (K)-specific demethylase 6A (kdm6a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa15362	XM_005167798.3	Danio rerio lysine (K)-specific demethylase 6A (kdm6a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

kdm6a ( XM_017357834.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213323.1 Danio rerio lysine (K)-specific demethylase 6A (kdm6a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa60364
Clone ID Related Accession (Same CDS sequence)	XM_017357834.1 , XM_017357834.2
Accession Version	XM_017357834.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	lysine-specific demethylase 6A isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCGT GCGGAGTGTC GGTGGCCGCT GTAGCTCGCG CTGCTGCTGC TGCTGCTGCT 
GGTGGTGATG AGGAAAAGAA AATGGCGGCG GGAAAAGCGA GAGAAACCGA GGAGGACTTT 
CCGAAGCTTA CGCCGCAAGA AAGGGAGCGC TTAGCCAGCA TAGACAGCAC ACTGTTTGGA 
TTTCAGAGGC TTCATGAAGA TGGCGCCAGA ACGAAGGCCT TGCTGTTAAA GGCTGTTCGC 
TGCTATGACG TCTATATTTT AAAAGCTGAA GGGAAAGTGG AGCCAGAGGT GTTTTGCCAG 
TTAGGTCACT TCAACCTTTT ACTGGAAGAT TATCCTAAAG CATTATCTGC ATACCAGAGA 
TACTACAGTT TACAGTCAGA CTACTGGAAG AATGCTGCCT TTTTATATGG CCTTGGTTTG 
GTTTACTTCC ATTACAATGC TTTTCGATGG GCAGTGCAGC TCACCCCCTC AGATGCCCAA 
TCTGGACAGA GAAGGGCGAT CAAAGCATTT CAGGAGGTGC TTTACATTGA CCCCTGCTTC 
TCAAGAGCCA AGGAGATCCA TCTGCGACTC GGTCTGATGT TTAAAGTCAA CACGGACTAT 
GAGTCAAGCC TCAAGCATTT TCAGCTGGCA TTGATTGACT CCACACCCTG CACTTTGTCC 
AAAGCTGAAA TTCAGTTCCA CATTGCTCAT GTATATGAAA TTCAGAAGAA ATACCGCATC 
GCAAAGGAAG CTTACGAGAG TCTGTTACAA ACAGAAAATC TCCCTGCACA GGTGAAGGCA 
ACTACGCTCC AACAATTAGG CTGGATGCAT CACACGGTCG AACAGTTGGG AGATAAAGCA 
AACAAGAACA GCTATGCTAT TCAGTGTCTG CAGAAATCCC TAGAAGCAGA CCCCAATTCT 
GGACAGTCTT GGTACTTCCT GGGCAGGTGC TACTCAAGTA TTGGGAAGGT TCAAGATGCC 
TTCATATCTT ACCGACAGTC TATTGATAAG TCTGAGGCCA GTGCAGATAC ATGGTGCTCA 
ATCGGTGTGT TGTATCAGCA GCAGAACCAG CCGATGGACG CACTGCAGGC ATATATCTGT 
GCAGTACAGC TGGACCACAG CCATGCGGCT GCCTGGATGG ACCTGGGCAC GCTCTACGAG 
TCCTGTAACC AGCCACAGGA TGCCATCAAG TGCTACATCA ATGCCACGTG CAGCAAGTCC 
TGCAGTAACA TCCCAGCTCT AACTGCTCGC ATCAAATGCC TCCAGCAAAC CACCTGCAAA 
GCTCATCAGA GCAATGAAGC CCAGTCTCAA ATCCCAAGCC AGTCTCTTCC TCCTCATGTG 
CTCTCTGGCC AGACAGAAGA CCAGTCCAGC CCGGCCAAGA AAAAGAGGAC CACTAGTCCA 
GCCAGGAATT CATCAGATTC CTGGACAAAT GATCCAGGTC ATCATCAGAT CTCCAACTGG 
AGTTTAACCC CACAGAAACT TCAGCTCCTG GAACAGTTAC GGAACAACAG AGCCAATCTG 
AAGCCAGTGC AGCTGCAGAT GCTGGAACAG TTGGAAAAGC AACTGAGTCT AATGCAACAG 
AATCAACAGA CAAGGCTGAA TGCCATGGGT CAGATACGAC CCAGTGTGTC CAACGGTCCT 
GTAGCCGACT CTTCACTGCC TACAAACTCT AATTCCACAC CTTCCCCTCA CCATCCACAC 
ATCGCTCTGT CTCGCACACC CTCTGCCCTC ACACGATGCG CTGCTCAGCC AATAGCCAAC 
GGACCTGTCC CTGCGAGCCC CGTCCCATGC AGCACCGCTG GTACCCTGGG TAACACAGAT 
GCCGTCTCTG TGGGCAATAA TCATCTCCCA GGATCGGGGA GCAACGGAAA TGTGCCTTAC 
TTGCGGCAAA ACGCACTACC TCATAACTGC ACAACCCCCA CCAGCAGCAG CATGGATGAT 
GAACAGTGGA AAAGCCAACA CATCAACTCC ACTCAGGGGC TTCAGAAAAG TCCAGGTTCA 
TATTCAGCAG GTCCTAACGG CGAGCAGCCT TTCTCTTCCA CTGGGACGTC CCAGCCTTCC 
CCGGCAGCCG GCACTGGCGG TCCAAATCAG GTCGGACATT CGGCCACAGC CAGCGACACG 
CAAGCGCAGG AGGCCAGTCA CAATCACCTC CCCTCCCCGA GCTCCCAGAG CTCTGCTACC 
TCAGGTGGAC AGCAAGGCAC GGTGAACACC AAAGAGAGCA AGCCTTCAGG AAACGGGCAT 
TCCGTGGGGA CGCCTAACAG CACTGCCACT GCAGAGGGAC TGCCTAATCA TGTCCATCAG 
GGCCAGGCAG ACACTGCTGG GGCGAAACCC CGTTCACCAG GTGTACTTAG TTCAGACAAT 
CCACAGCTCT CGGCCTTGTT GATTGGAAAA GCCAATGATA GTAATAATAG TAATGGAGGA 
GGGTCGGCGG CTGCTGGCAC AAAGGTTAAC AATGTTCACC CGAGTCTGCA CAAGCCGCAG 
GAAAACTCGG TGGCTTCTTC TCCATGTTCT GCCATGTCTG CAGCTTCGCC CTCACCCAAA 
ACTGCAGACC ACCACAGTAC TCAGAACAGT GTTAACAGCC TTAACAGCCC CACGCTCAAC 
GGCAAAGGTC TGGAGGACTC TCAGAGCCCC TTGAAGGTGG CTTCTCCACT GGTTTGTCAT 
AAACCAACGC CTCCGTCGTT CACTCTGTGG TCCTCTGTGT CCATCTACCC CAGCTCCAGT 
GATGTGCTCA AAGCATGCAG AAACCTGGGG AAGAATGGTG TGTCCAATAA CAGCATCTTA 
CTAGACAAGT GTCCCCCTCC ACGGTTACCA CCTCCACCCA TCCCTCCTCT GCCAAAGGAC 
AAACTCAATC CTCCAACCCC TAGTATTTAT TTGGAGAACA AGAGGGATGC TTTCTTTCCA 
CCGCTGCACC AGTTCTGCAC AAACACAGCC AACCCTGTAA CAGTGATCCG TGGCCTGGCT 
GGAGCACTCA AGCTAGACCT TGGATTATTC TCCACCAAGA CTCTGGTTGA GGCTAACCCA 
GAGCACCTTG TGGAGGTGAG GACTCAGTTG TCTCAGCCCA CCGATGAGAA CTGGGATGTG 
ACAGGGAGCA GGAAGATGTG GCGGTGTGAG AGCAGTCGGT CTCACACCAC CATTGCCAGA 
TATGCTCAAT ACCAGGCCTC ATCTTTCCAG GAATCATTGC GGGAGGAAAA TGAAAAGAAA 
GGACAGAAAG ACCACTCGGA TACGGAGTCT GCTCCATCAG AAAATGTGGT GCGGAGAAGA 
AGAGGTCCCT TTAAGCACAT CAAATTTGGA ACCAACATTG ATTTGTCAGA TGAGAAAAAG 
TGGAAGCTGC AGCTGGCTGA GTTGAGTAAA CTGCCTGCCT TCGCACGGGT GGTGTCTGCA 
GGGAATCTGC TCAGTCATGT GGGCCACACA ATTCTCGGCA TGAACACAGT CCAGCTCTAT 
ATGAAGGTCC CGGGCAGCAG GACACCAGGT CATCAAGAAA ACAACAACTT CTGCTCTGTG 
AACATAAACA TTGGCCCTGG AGATTGTGAA TGGTTTGCCG TTCCTGAGCC GTACTGGGGT 
GTCATGAATG ATTTCTGTGA AAAGAATAAT ATTAATTATC TAATGGGATC TTGGTGGCCA 
AATCTTGAGG ACTTGTATGA GGCAAATGTT CCAGTGTATC GCTTTATCCA GCGACCAGGT 
GATCTGGTCT GGCTCAATAC AGGGACTGTG CATTGGGTTC AGGCTATTGG CTGGTGCAAC 
AACATTGCCT GGAACGTAGG ACCACTCACT GTGCATCAGT ACAAGCTAGC GGTGGAGCGC 
TATGAGTGGA ACAAACTCCA GAGTGTCAAA TCCATCGTTC CCATGATTCA CCTCTCCTGG 
AATATGGCCA GAAACATCAA AGTGTCAGAC CACAAGCTCT TTGAGATGAT CAAGTACTGT 
TTATTGAGGA CACTAAAGCA GTGTCAGATG CTGAGGGAGG CTCTCATAGC TGCTGGAAAA 
GAGCTTGTTT GGCATGGAAG GACCAAGGAC GAACCTGCTC ACTACTGTAG CATCTGTGAG 
GTGGAGGTCT TTGATCTGCT GTTTGTCACC AGTGAAAGTA ACTCAAGAAA GACGTATGTG 
GTTCACTGCC AGGGCTGCGC TCGCAGATGT AGCCCTAACC TGGACAACTT TGTGGTTTTA 
GAACAGTACA AAATAGACGA CCTCATGCAA GTGTACGACC AGTTCACATT AGCTCCACCT 
CTGCCCTCCT CCTCCTCATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213323.1
CDS	623..4846
Translation	MKSCGVSVAAVARAAAAAAAGGDEEKKMAAGKARETEEDFPKLTPQERERLASIDSTLFGFQRLHEDGARTKALLLKAVRCYDVYILKAEGKVEPEVFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFRWAVQLTPSDAQSGQRRAIKAFQEVLYIDPCFSRAKEIHLRLGLMFKVNTDYESSLKHFQLALIDSTPCTLSKAEIQFHIAHVYEIQKKYRIAKEAYESLLQTENLPAQVKATTLQQLGWMHHTVEQLGDKANKNSYAIQCLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHSHAAAWMDLGTLYESCNQPQDAIKCYINATCSKSCSNIPALTARIKCLQQTTCKAHQSNEAQSQIPSQSLPPHVLSGQTEDQSSPAKKKRTTSPARNSSDSWTNDPGHHQISNWSLTPQKLQLLEQLRNNRANLKPVQLQMLEQLEKQLSLMQQNQQTRLNAMGQIRPSVSNGPVADSSLPTNSNSTPSPHHPHIALSRTPSALTRCAAQPIANGPVPASPVPCSTAGTLGNTDAVSVGNNHLPGSGSNGNVPYLRQNALPHNCTTPTSSSMDDEQWKSQHINSTQGLQKSPGSYSAGPNGEQPFSSTGTSQPSPAAGTGGPNQVGHSATASDTQAQEASHNHLPSPSSQSSATSGGQQGTVNTKESKPSGNGHSVGTPNSTATAEGLPNHVHQGQADTAGAKPRSPGVLSSDNPQLSALLIGKANDSNNSNGGGSAAAGTKVNNVHPSLHKPQENSVASSPCSAMSAASPSPKTADHHSTQNSVNSLNSPTLNGKGLEDSQSPLKVASPLVCHKPTPPSFTLWSSVSIYPSSSDVLKACRNLGKNGVSNNSILLDKCPPPRLPPPPIPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNTANPVTVIRGLAGALKLDLGLFSTKTLVEANPEHLVEVRTQLSQPTDENWDVTGSRKMWRCESSRSHTTIARYAQYQASSFQESLREENEKKGQKDHSDTESAPSENVVRRRRGPFKHIKFGTNIDLSDEKKWKLQLAELSKLPAFARVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVPEPYWGVMNDFCEKNNINYLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWLNTGTVHWVQAIGWCNNIAWNVGPLTVHQYKLAVERYEWNKLQSVKSIVPMIHLSWNMARNIKVSDHKLFEMIKYCLLRTLKQCQMLREALIAAGKELVWHGRTKDEPAHYCSICEVEVFDLLFVTSESNSRKTYVVHCQGCARRCSPNLDNFVVLEQYKIDDLMQVYDQFTLAPPLPSSSSS

Target ORF information:

RefSeq Version	XM_017357834.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 6A (kdm6a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357834.1

ORF Insert Sequence:

ATGAAATCGT GCGGAGTGTC GGTGGCCGCT GTAGCTCGCG CTGCTGCTGC TGCTGCTGCT 
GGTGGTGATG AGGAAAAGAA AATGGCGGCG GGAAAAGCGA GAGAAACCGA GGAGGACTTT 
CCGAAGCTTA CGCCGCAAGA AAGGGAGCGC TTAGCCAGCA TAGACAGCAC ACTGTTTGGA 
TTTCAGAGGC TTCATGAAGA TGGCGCCAGA ACGAAGGCCT TGCTGTTAAA GGCTGTTCGC 
TGCTATGACG TCTATATTTT AAAAGCTGAA GGGAAAGTGG AGCCAGAGGT GTTTTGCCAG 
TTAGGTCACT TCAACCTTTT ACTGGAAGAT TATCCTAAAG CATTATCTGC ATACCAGAGA 
TACTACAGTT TACAGTCAGA CTACTGGAAG AATGCTGCCT TTTTATATGG CCTTGGTTTG 
GTTTACTTCC ATTACAATGC TTTTCGATGG GCAGTGCAGC TCACCCCCTC AGATGCCCAA 
TCTGGACAGA GAAGGGCGAT CAAAGCATTT CAGGAGGTGC TTTACATTGA CCCCTGCTTC 
TCAAGAGCCA AGGAGATCCA TCTGCGACTC GGTCTGATGT TTAAAGTCAA CACGGACTAT 
GAGTCAAGCC TCAAGCATTT TCAGCTGGCA TTGATTGACT CCACACCCTG CACTTTGTCC 
AAAGCTGAAA TTCAGTTCCA CATTGCTCAT GTATATGAAA TTCAGAAGAA ATACCGCATC 
GCAAAGGAAG CTTACGAGAG TCTGTTACAA ACAGAAAATC TCCCTGCACA GGTGAAGGCA 
ACTACGCTCC AACAATTAGG CTGGATGCAT CACACGGTCG AACAGTTGGG AGATAAAGCA 
AACAAGAACA GCTATGCTAT TCAGTGTCTG CAGAAATCCC TAGAAGCAGA CCCCAATTCT 
GGACAGTCTT GGTACTTCCT GGGCAGGTGC TACTCAAGTA TTGGGAAGGT TCAAGATGCC 
TTCATATCTT ACCGACAGTC TATTGATAAG TCTGAGGCCA GTGCAGATAC ATGGTGCTCA 
ATCGGTGTGT TGTATCAGCA GCAGAACCAG CCGATGGACG CACTGCAGGC ATATATCTGT 
GCAGTACAGC TGGACCACAG CCATGCGGCT GCCTGGATGG ACCTGGGCAC GCTCTACGAG 
TCCTGTAACC AGCCACAGGA TGCCATCAAG TGCTACATCA ATGCCACGTG CAGCAAGTCC 
TGCAGTAACA TCCCAGCTCT AACTGCTCGC ATCAAATGCC TCCAGCAAAC CACCTGCAAA 
GCTCATCAGA GCAATGAAGC CCAGTCTCAA ATCCCAAGCC AGTCTCTTCC TCCTCATGTG 
CTCTCTGGCC AGACAGAAGA CCAGTCCAGC CCGGCCAAGA AAAAGAGGAC CACTAGTCCA 
GCCAGGAATT CATCAGATTC CTGGACAAAT GATCCAGGTC ATCATCAGAT CTCCAACTGG 
AGTTTAACCC CACAGAAACT TCAGCTCCTG GAACAGTTAC GGAACAACAG AGCCAATCTG 
AAGCCAGTGC AGCTGCAGAT GCTGGAACAG TTGGAAAAGC AACTGAGTCT AATGCAACAG 
AATCAACAGA CAAGGCTGAA TGCCATGGGT CAGATACGAC CCAGTGTGTC CAACGGTCCT 
GTAGCCGACT CTTCACTGCC TACAAACTCT AATTCCACAC CTTCCCCTCA CCATCCACAC 
ATCGCTCTGT CTCGCACACC CTCTGCCCTC ACACGATGCG CTGCTCAGCC AATAGCCAAC 
GGACCTGTCC CTGCGAGCCC CGTCCCATGC AGCACCGCTG GTACCCTGGG TAACACAGAT 
GCCGTCTCTG TGGGCAATAA TCATCTCCCA GGATCGGGGA GCAACGGAAA TGTGCCTTAC 
TTGCGGCAAA ACGCACTACC TCATAACTGC ACAACCCCCA CCAGCAGCAG CATGGATGAT 
GAACAGTGGA AAAGCCAACA CATCAACTCC ACTCAGGGGC TTCAGAAAAG TCCAGGTTCA 
TATTCAGCAG GTCCTAACGG CGAGCAGCCT TTCTCTTCCA CTGGGACGTC CCAGCCTTCC 
CCGGCAGCCG GCACTGGCGG TCCAAATCAG GTCGGACATT CGGCCACAGC CAGCGACACG 
CAAGCGCAGG AGGCCAGTCA CAATCACCTC CCCTCCCCGA GCTCCCAGAG CTCTGCTACC 
TCAGGTGGAC AGCAAGGCAC GGTGAACACC AAAGAGAGCA AGCCTTCAGG AAACGGGCAT 
TCCGTGGGGA CGCCTAACAG CACTGCCACT GCAGAGGGAC TGCCTAATCA TGTCCATCAG 
GGCCAGGCAG ACACTGCTGG GGCGAAACCC CGTTCACCAG GTGTACTTAG TTCAGACAAT 
CCACAGCTCT CGGCCTTGTT GATTGGAAAA GCCAATGATA GTAATAATAG TAATGGAGGA 
GGGTCGGCGG CTGCTGGCAC AAAGGTTAAC AATGTTCACC CGAGTCTGCA CAAGCCGCAG 
GAAAACTCGG TGGCTTCTTC TCCATGTTCT GCCATGTCTG CAGCTTCGCC CTCACCCAAA 
ACTGCAGACC ACCACAGTAC TCAGAACAGT GTTAACAGCC TTAACAGCCC CACGCTCAAC 
GGCAAAGGTC TGGAGGACTC TCAGAGCCCC TTGAAGGTGG CTTCTCCACT GGTTTGTCAT 
AAACCAACGC CTCCGTCGTT CACTCTGTGG TCCTCTGTGT CCATCTACCC CAGCTCCAGT 
GATGTGCTCA AAGCATGCAG AAACCTGGGG AAGAATGGTG TGTCCAATAA CAGCATCTTA 
CTAGACAAGT GTCCCCCTCC ACGGTTACCA CCTCCACCCA TCCCTCCTCT GCCAAAGGAC 
AAACTCAATC CTCCAACCCC TAGTATTTAT TTGGAGAACA AGAGGGATGC TTTCTTTCCA 
CCGCTGCACC AGTTCTGCAC AAACACAGCC AACCCTGTAA CAGTGATCCG TGGCCTGGCT 
GGAGCACTCA AGCTAGACCT TGGATTATTC TCCACCAAGA CTCTGGTTGA GGCTAACCCA 
GAGCACCTTG TGGAGGTGAG GACTCAGTTG TCTCAGCCCA CCGATGAGAA CTGGGATGTG 
ACAGGGAGCA GGAAGATGTG GCGGTGTGAG AGCAGTCGGT CTCACACCAC CATTGCCAGA 
TATGCTCAAT ACCAGGCCTC ATCTTTCCAG GAATCATTGC GGGAGGAAAA TGAAAAGAAA 
GGACAGAAAG ACCACTCGGA TACGGAGTCT GCTCCATCAG AAAATGTGGT GCGGAGAAGA 
AGAGGTCCCT TTAAGCACAT CAAATTTGGA ACCAACATTG ATTTGTCAGA TGAGAAAAAG 
TGGAAGCTGC AGCTGGCTGA GTTGAGTAAA CTGCCTGCCT TCGCACGGGT GGTGTCTGCA 
GGGAATCTGC TCAGTCATGT GGGCCACACA ATTCTCGGCA TGAACACAGT CCAGCTCTAT 
ATGAAGGTCC CGGGCAGCAG GACACCAGGT CATCAAGAAA ACAACAACTT CTGCTCTGTG 
AACATAAACA TTGGCCCTGG AGATTGTGAA TGGTTTGCCG TTCCTGAGCC GTACTGGGGT 
GTCATGAATG ATTTCTGTGA AAAGAATAAT ATTAATTATC TAATGGGATC TTGGTGGCCA 
AATCTTGAGG ACTTGTATGA GGCAAATGTT CCAGTGTATC GCTTTATCCA GCGACCAGGT 
GATCTGGTCT GGCTCAATAC AGGGACTGTG CATTGGGTTC AGGCTATTGG CTGGTGCAAC 
AACATTGCCT GGAACGTAGG ACCACTCACT GTGCATCAGT ACAAGCTAGC GGTGGAGCGC 
TATGAGTGGA ACAAACTCCA GAGTGTCAAA TCCATCGTTC CCATGATTCA CCTCTCCTGG 
AATATGGCCA GAAACATCAA AGTGTCAGAC CACAAGCTCT TTGAGATGAT CAAGTACTGT 
TTATTGAGGA CACTAAAGCA GTGTCAGATG CTGAGGGAGG CTCTCATAGC TGCTGGAAAA 
GAGCTTGTTT GGCATGGAAG GACCAAGGAC GAACCTGCTC ACTACTGTAG CATCTGTGAG 
GTGGAGGTCT TTGATCTGCT GTTTGTCACC AGTGAAAGTA ACTCAAGAAA GACGTATGTG 
GTTCACTGCC AGGGCTGCGC TCGCAGATGT AGCCCTAACC TGGACAACTT TGTGGTTTTA 
GAACAGTACA AAATAGACGA CCTCATGCAA GTGTACGACC AGTTCACATT AGCTCCACCT 
CTGCCCTCCT CCTCCTCATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm6a ( XM_017357834.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213323.1 Danio rerio lysine (K)-specific demethylase 6A (kdm6a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa60364
Clone ID Related Accession (Same CDS sequence)	XM_017357834.1 , XM_017357834.2
Accession Version	XM_017357834.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4224bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	lysine-specific demethylase 6A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357834.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCGT GCGGAGTGTC GGTGGCCGCT GTAGCTCGCG CTGCTGCTGC TGCTGCTGCT 
GGTGGTGATG AGGAAAAGAA AATGGCGGCG GGAAAAGCGA GAGAAACCGA GGAGGACTTT 
CCGAAGCTTA CGCCGCAAGA AAGGGAGCGC TTAGCCAGCA TAGACAGCAC ACTGTTTGGA 
TTTCAGAGGC TTCATGAAGA TGGCGCCAGA ACGAAGGCCT TGCTGTTAAA GGCTGTTCGC 
TGCTATGACG TCTATATTTT AAAAGCTGAA GGGAAAGTGG AGCCAGAGGT GTTTTGCCAG 
TTAGGTCACT TCAACCTTTT ACTGGAAGAT TATCCTAAAG CATTATCTGC ATACCAGAGA 
TACTACAGTT TACAGTCAGA CTACTGGAAG AATGCTGCCT TTTTATATGG CCTTGGTTTG 
GTTTACTTCC ATTACAATGC TTTTCGATGG GCAGTGCAGC TCACCCCCTC AGATGCCCAA 
TCTGGACAGA GAAGGGCGAT CAAAGCATTT CAGGAGGTGC TTTACATTGA CCCCTGCTTC 
TCAAGAGCCA AGGAGATCCA TCTGCGACTC GGTCTGATGT TTAAAGTCAA CACGGACTAT 
GAGTCAAGCC TCAAGCATTT TCAGCTGGCA TTGATTGACT CCACACCCTG CACTTTGTCC 
AAAGCTGAAA TTCAGTTCCA CATTGCTCAT GTATATGAAA TTCAGAAGAA ATACCGCATC 
GCAAAGGAAG CTTACGAGAG TCTGTTACAA ACAGAAAATC TCCCTGCACA GGTGAAGGCA 
ACTACGCTCC AACAATTAGG CTGGATGCAT CACACGGTCG AACAGTTGGG AGATAAAGCA 
AACAAGAACA GCTATGCTAT TCAGTGTCTG CAGAAATCCC TAGAAGCAGA CCCCAATTCT 
GGACAGTCTT GGTACTTCCT GGGCAGGTGC TACTCAAGTA TTGGGAAGGT TCAAGATGCC 
TTCATATCTT ACCGACAGTC TATTGATAAG TCTGAGGCCA GTGCAGATAC ATGGTGCTCA 
ATCGGTGTGT TGTATCAGCA GCAGAACCAG CCGATGGACG CACTGCAGGC ATATATCTGT 
GCAGTACAGC TGGACCACAG CCATGCGGCT GCCTGGATGG ACCTGGGCAC GCTCTACGAG 
TCCTGTAACC AGCCACAGGA TGCCATCAAG TGCTACATCA ATGCCACGTG CAGCAAGTCC 
TGCAGTAACA TCCCAGCTCT AACTGCTCGC ATCAAATGCC TCCAGCAAAC CACCTGCAAA 
GCTCATCAGA GCAATGAAGC CCAGTCTCAA ATCCCAAGCC AGTCTCTTCC TCCTCATGTG 
CTCTCTGGCC AGACAGAAGA CCAGTCCAGC CCGGCCAAGA AAAAGAGGAC CACTAGTCCA 
GCCAGGAATT CATCAGATTC CTGGACAAAT GATCCAGGTC ATCATCAGAT CTCCAACTGG 
AGTTTAACCC CACAGAAACT TCAGCTCCTG GAACAGTTAC GGAACAACAG AGCCAATCTG 
AAGCCAGTGC AGCTGCAGAT GCTGGAACAG TTGGAAAAGC AACTGAGTCT AATGCAACAG 
AATCAACAGA CAAGGCTGAA TGCCATGGGT CAGATACGAC CCAGTGTGTC CAACGGTCCT 
GTAGCCGACT CTTCACTGCC TACAAACTCT AATTCCACAC CTTCCCCTCA CCATCCACAC 
ATCGCTCTGT CTCGCACACC CTCTGCCCTC ACACGATGCG CTGCTCAGCC AATAGCCAAC 
GGACCTGTCC CTGCGAGCCC CGTCCCATGC AGCACCGCTG GTACCCTGGG TAACACAGAT 
GCCGTCTCTG TGGGCAATAA TCATCTCCCA GGATCGGGGA GCAACGGAAA TGTGCCTTAC 
TTGCGGCAAA ACGCACTACC TCATAACTGC ACAACCCCCA CCAGCAGCAG CATGGATGAT 
GAACAGTGGA AAAGCCAACA CATCAACTCC ACTCAGGGGC TTCAGAAAAG TCCAGGTTCA 
TATTCAGCAG GTCCTAACGG CGAGCAGCCT TTCTCTTCCA CTGGGACGTC CCAGCCTTCC 
CCGGCAGCCG GCACTGGCGG TCCAAATCAG GTCGGACATT CGGCCACAGC CAGCGACACG 
CAAGCGCAGG AGGCCAGTCA CAATCACCTC CCCTCCCCGA GCTCCCAGAG CTCTGCTACC 
TCAGGTGGAC AGCAAGGCAC GGTGAACACC AAAGAGAGCA AGCCTTCAGG AAACGGGCAT 
TCCGTGGGGA CGCCTAACAG CACTGCCACT GCAGAGGGAC TGCCTAATCA TGTCCATCAG 
GGCCAGGCAG ACACTGCTGG GGCGAAACCC CGTTCACCAG GTGTACTTAG TTCAGACAAT 
CCACAGCTCT CGGCCTTGTT GATTGGAAAA GCCAATGATA GTAATAATAG TAATGGAGGA 
GGGTCGGCGG CTGCTGGCAC AAAGGTTAAC AATGTTCACC CGAGTCTGCA CAAGCCGCAG 
GAAAACTCGG TGGCTTCTTC TCCATGTTCT GCCATGTCTG CAGCTTCGCC CTCACCCAAA 
ACTGCAGACC ACCACAGTAC TCAGAACAGT GTTAACAGCC TTAACAGCCC CACGCTCAAC 
GGCAAAGGTC TGGAGGACTC TCAGAGCCCC TTGAAGGTGG CTTCTCCACT GGTTTGTCAT 
AAACCAACGC CTCCGTCGTT CACTCTGTGG TCCTCTGTGT CCATCTACCC CAGCTCCAGT 
GATGTGCTCA AAGCATGCAG AAACCTGGGG AAGAATGGTG TGTCCAATAA CAGCATCTTA 
CTAGACAAGT GTCCCCCTCC ACGGTTACCA CCTCCACCCA TCCCTCCTCT GCCAAAGGAC 
AAACTCAATC CTCCAACCCC TAGTATTTAT TTGGAGAACA AGAGGGATGC TTTCTTTCCA 
CCGCTGCACC AGTTCTGCAC AAACACAGCC AACCCTGTAA CAGTGATCCG TGGCCTGGCT 
GGAGCACTCA AGCTAGACCT TGGATTATTC TCCACCAAGA CTCTGGTTGA GGCTAACCCA 
GAGCACCTTG TGGAGGTGAG GACTCAGTTG TCTCAGCCCA CCGATGAGAA CTGGGATGTG 
ACAGGGAGCA GGAAGATGTG GCGGTGTGAG AGCAGTCGGT CTCACACCAC CATTGCCAGA 
TATGCTCAAT ACCAGGCCTC ATCTTTCCAG GAATCATTGC GGGAGGAAAA TGAAAAGAAA 
GGACAGAAAG ACCACTCGGA TACGGAGTCT GCTCCATCAG AAAATGTGGT GCGGAGAAGA 
AGAGGTCCCT TTAAGCACAT CAAATTTGGA ACCAACATTG ATTTGTCAGA TGAGAAAAAG 
TGGAAGCTGC AGCTGGCTGA GTTGAGTAAA CTGCCTGCCT TCGCACGGGT GGTGTCTGCA 
GGGAATCTGC TCAGTCATGT GGGCCACACA ATTCTCGGCA TGAACACAGT CCAGCTCTAT 
ATGAAGGTCC CGGGCAGCAG GACACCAGGT CATCAAGAAA ACAACAACTT CTGCTCTGTG 
AACATAAACA TTGGCCCTGG AGATTGTGAA TGGTTTGCCG TTCCTGAGCC GTACTGGGGT 
GTCATGAATG ATTTCTGTGA AAAGAATAAT ATTAATTATC TAATGGGATC TTGGTGGCCA 
AATCTTGAGG ACTTGTATGA GGCAAATGTT CCAGTGTATC GCTTTATCCA GCGACCAGGT 
GATCTGGTCT GGCTCAATAC AGGGACTGTG CATTGGGTTC AGGCTATTGG CTGGTGCAAC 
AACATTGCCT GGAACGTAGG ACCACTCACT GTGCATCAGT ACAAGCTAGC GGTGGAGCGC 
TATGAGTGGA ACAAACTCCA GAGTGTCAAA TCCATCGTTC CCATGATTCA CCTCTCCTGG 
AATATGGCCA GAAACATCAA AGTGTCAGAC CACAAGCTCT TTGAGATGAT CAAGTACTGT 
TTATTGAGGA CACTAAAGCA GTGTCAGATG CTGAGGGAGG CTCTCATAGC TGCTGGAAAA 
GAGCTTGTTT GGCATGGAAG GACCAAGGAC GAACCTGCTC ACTACTGTAG CATCTGTGAG 
GTGGAGGTCT TTGATCTGCT GTTTGTCACC AGTGAAAGTA ACTCAAGAAA GACGTATGTG 
GTTCACTGCC AGGGCTGCGC TCGCAGATGT AGCCCTAACC TGGACAACTT TGTGGTTTTA 
GAACAGTACA AAATAGACGA CCTCATGCAA GTGTACGACC AGTTCACATT AGCTCCACCT 
CTGCCCTCCT CCTCCTCATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213323.1
CDS	469..4692
Translation	MKSCGVSVAAVARAAAAAAAGGDEEKKMAAGKARETEEDFPKLTPQERERLASIDSTLFGFQRLHEDGARTKALLLKAVRCYDVYILKAEGKVEPEVFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFRWAVQLTPSDAQSGQRRAIKAFQEVLYIDPCFSRAKEIHLRLGLMFKVNTDYESSLKHFQLALIDSTPCTLSKAEIQFHIAHVYEIQKKYRIAKEAYESLLQTENLPAQVKATTLQQLGWMHHTVEQLGDKANKNSYAIQCLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHSHAAAWMDLGTLYESCNQPQDAIKCYINATCSKSCSNIPALTARIKCLQQTTCKAHQSNEAQSQIPSQSLPPHVLSGQTEDQSSPAKKKRTTSPARNSSDSWTNDPGHHQISNWSLTPQKLQLLEQLRNNRANLKPVQLQMLEQLEKQLSLMQQNQQTRLNAMGQIRPSVSNGPVADSSLPTNSNSTPSPHHPHIALSRTPSALTRCAAQPIANGPVPASPVPCSTAGTLGNTDAVSVGNNHLPGSGSNGNVPYLRQNALPHNCTTPTSSSMDDEQWKSQHINSTQGLQKSPGSYSAGPNGEQPFSSTGTSQPSPAAGTGGPNQVGHSATASDTQAQEASHNHLPSPSSQSSATSGGQQGTVNTKESKPSGNGHSVGTPNSTATAEGLPNHVHQGQADTAGAKPRSPGVLSSDNPQLSALLIGKANDSNNSNGGGSAAAGTKVNNVHPSLHKPQENSVASSPCSAMSAASPSPKTADHHSTQNSVNSLNSPTLNGKGLEDSQSPLKVASPLVCHKPTPPSFTLWSSVSIYPSSSDVLKACRNLGKNGVSNNSILLDKCPPPRLPPPPIPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNTANPVTVIRGLAGALKLDLGLFSTKTLVEANPEHLVEVRTQLSQPTDENWDVTGSRKMWRCESSRSHTTIARYAQYQASSFQESLREENEKKGQKDHSDTESAPSENVVRRRRGPFKHIKFGTNIDLSDEKKWKLQLAELSKLPAFARVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVPEPYWGVMNDFCEKNNINYLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWLNTGTVHWVQAIGWCNNIAWNVGPLTVHQYKLAVERYEWNKLQSVKSIVPMIHLSWNMARNIKVSDHKLFEMIKYCLLRTLKQCQMLREALIAAGKELVWHGRTKDEPAHYCSICEVEVFDLLFVTSESNSRKTYVVHCQGCARRCSPNLDNFVVLEQYKIDDLMQVYDQFTLAPPLPSSSSS

Target ORF information:

RefSeq Version	XM_017357834.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 6A (kdm6a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357834.2

ORF Insert Sequence:

ATGAAATCGT GCGGAGTGTC GGTGGCCGCT GTAGCTCGCG CTGCTGCTGC TGCTGCTGCT 
GGTGGTGATG AGGAAAAGAA AATGGCGGCG GGAAAAGCGA GAGAAACCGA GGAGGACTTT 
CCGAAGCTTA CGCCGCAAGA AAGGGAGCGC TTAGCCAGCA TAGACAGCAC ACTGTTTGGA 
TTTCAGAGGC TTCATGAAGA TGGCGCCAGA ACGAAGGCCT TGCTGTTAAA GGCTGTTCGC 
TGCTATGACG TCTATATTTT AAAAGCTGAA GGGAAAGTGG AGCCAGAGGT GTTTTGCCAG 
TTAGGTCACT TCAACCTTTT ACTGGAAGAT TATCCTAAAG CATTATCTGC ATACCAGAGA 
TACTACAGTT TACAGTCAGA CTACTGGAAG AATGCTGCCT TTTTATATGG CCTTGGTTTG 
GTTTACTTCC ATTACAATGC TTTTCGATGG GCAGTGCAGC TCACCCCCTC AGATGCCCAA 
TCTGGACAGA GAAGGGCGAT CAAAGCATTT CAGGAGGTGC TTTACATTGA CCCCTGCTTC 
TCAAGAGCCA AGGAGATCCA TCTGCGACTC GGTCTGATGT TTAAAGTCAA CACGGACTAT 
GAGTCAAGCC TCAAGCATTT TCAGCTGGCA TTGATTGACT CCACACCCTG CACTTTGTCC 
AAAGCTGAAA TTCAGTTCCA CATTGCTCAT GTATATGAAA TTCAGAAGAA ATACCGCATC 
GCAAAGGAAG CTTACGAGAG TCTGTTACAA ACAGAAAATC TCCCTGCACA GGTGAAGGCA 
ACTACGCTCC AACAATTAGG CTGGATGCAT CACACGGTCG AACAGTTGGG AGATAAAGCA 
AACAAGAACA GCTATGCTAT TCAGTGTCTG CAGAAATCCC TAGAAGCAGA CCCCAATTCT 
GGACAGTCTT GGTACTTCCT GGGCAGGTGC TACTCAAGTA TTGGGAAGGT TCAAGATGCC 
TTCATATCTT ACCGACAGTC TATTGATAAG TCTGAGGCCA GTGCAGATAC ATGGTGCTCA 
ATCGGTGTGT TGTATCAGCA GCAGAACCAG CCGATGGACG CACTGCAGGC ATATATCTGT 
GCAGTACAGC TGGACCACAG CCATGCGGCT GCCTGGATGG ACCTGGGCAC GCTCTACGAG 
TCCTGTAACC AGCCACAGGA TGCCATCAAG TGCTACATCA ATGCCACGTG CAGCAAGTCC 
TGCAGTAACA TCCCAGCTCT AACTGCTCGC ATCAAATGCC TCCAGCAAAC CACCTGCAAA 
GCTCATCAGA GCAATGAAGC CCAGTCTCAA ATCCCAAGCC AGTCTCTTCC TCCTCATGTG 
CTCTCTGGCC AGACAGAAGA CCAGTCCAGC CCGGCCAAGA AAAAGAGGAC CACTAGTCCA 
GCCAGGAATT CATCAGATTC CTGGACAAAT GATCCAGGTC ATCATCAGAT CTCCAACTGG 
AGTTTAACCC CACAGAAACT TCAGCTCCTG GAACAGTTAC GGAACAACAG AGCCAATCTG 
AAGCCAGTGC AGCTGCAGAT GCTGGAACAG TTGGAAAAGC AACTGAGTCT AATGCAACAG 
AATCAACAGA CAAGGCTGAA TGCCATGGGT CAGATACGAC CCAGTGTGTC CAACGGTCCT 
GTAGCCGACT CTTCACTGCC TACAAACTCT AATTCCACAC CTTCCCCTCA CCATCCACAC 
ATCGCTCTGT CTCGCACACC CTCTGCCCTC ACACGATGCG CTGCTCAGCC AATAGCCAAC 
GGACCTGTCC CTGCGAGCCC CGTCCCATGC AGCACCGCTG GTACCCTGGG TAACACAGAT 
GCCGTCTCTG TGGGCAATAA TCATCTCCCA GGATCGGGGA GCAACGGAAA TGTGCCTTAC 
TTGCGGCAAA ACGCACTACC TCATAACTGC ACAACCCCCA CCAGCAGCAG CATGGATGAT 
GAACAGTGGA AAAGCCAACA CATCAACTCC ACTCAGGGGC TTCAGAAAAG TCCAGGTTCA 
TATTCAGCAG GTCCTAACGG CGAGCAGCCT TTCTCTTCCA CTGGGACGTC CCAGCCTTCC 
CCGGCAGCCG GCACTGGCGG TCCAAATCAG GTCGGACATT CGGCCACAGC CAGCGACACG 
CAAGCGCAGG AGGCCAGTCA CAATCACCTC CCCTCCCCGA GCTCCCAGAG CTCTGCTACC 
TCAGGTGGAC AGCAAGGCAC GGTGAACACC AAAGAGAGCA AGCCTTCAGG AAACGGGCAT 
TCCGTGGGGA CGCCTAACAG CACTGCCACT GCAGAGGGAC TGCCTAATCA TGTCCATCAG 
GGCCAGGCAG ACACTGCTGG GGCGAAACCC CGTTCACCAG GTGTACTTAG TTCAGACAAT 
CCACAGCTCT CGGCCTTGTT GATTGGAAAA GCCAATGATA GTAATAATAG TAATGGAGGA 
GGGTCGGCGG CTGCTGGCAC AAAGGTTAAC AATGTTCACC CGAGTCTGCA CAAGCCGCAG 
GAAAACTCGG TGGCTTCTTC TCCATGTTCT GCCATGTCTG CAGCTTCGCC CTCACCCAAA 
ACTGCAGACC ACCACAGTAC TCAGAACAGT GTTAACAGCC TTAACAGCCC CACGCTCAAC 
GGCAAAGGTC TGGAGGACTC TCAGAGCCCC TTGAAGGTGG CTTCTCCACT GGTTTGTCAT 
AAACCAACGC CTCCGTCGTT CACTCTGTGG TCCTCTGTGT CCATCTACCC CAGCTCCAGT 
GATGTGCTCA AAGCATGCAG AAACCTGGGG AAGAATGGTG TGTCCAATAA CAGCATCTTA 
CTAGACAAGT GTCCCCCTCC ACGGTTACCA CCTCCACCCA TCCCTCCTCT GCCAAAGGAC 
AAACTCAATC CTCCAACCCC TAGTATTTAT TTGGAGAACA AGAGGGATGC TTTCTTTCCA 
CCGCTGCACC AGTTCTGCAC AAACACAGCC AACCCTGTAA CAGTGATCCG TGGCCTGGCT 
GGAGCACTCA AGCTAGACCT TGGATTATTC TCCACCAAGA CTCTGGTTGA GGCTAACCCA 
GAGCACCTTG TGGAGGTGAG GACTCAGTTG TCTCAGCCCA CCGATGAGAA CTGGGATGTG 
ACAGGGAGCA GGAAGATGTG GCGGTGTGAG AGCAGTCGGT CTCACACCAC CATTGCCAGA 
TATGCTCAAT ACCAGGCCTC ATCTTTCCAG GAATCATTGC GGGAGGAAAA TGAAAAGAAA 
GGACAGAAAG ACCACTCGGA TACGGAGTCT GCTCCATCAG AAAATGTGGT GCGGAGAAGA 
AGAGGTCCCT TTAAGCACAT CAAATTTGGA ACCAACATTG ATTTGTCAGA TGAGAAAAAG 
TGGAAGCTGC AGCTGGCTGA GTTGAGTAAA CTGCCTGCCT TCGCACGGGT GGTGTCTGCA 
GGGAATCTGC TCAGTCATGT GGGCCACACA ATTCTCGGCA TGAACACAGT CCAGCTCTAT 
ATGAAGGTCC CGGGCAGCAG GACACCAGGT CATCAAGAAA ACAACAACTT CTGCTCTGTG 
AACATAAACA TTGGCCCTGG AGATTGTGAA TGGTTTGCCG TTCCTGAGCC GTACTGGGGT 
GTCATGAATG ATTTCTGTGA AAAGAATAAT ATTAATTATC TAATGGGATC TTGGTGGCCA 
AATCTTGAGG ACTTGTATGA GGCAAATGTT CCAGTGTATC GCTTTATCCA GCGACCAGGT 
GATCTGGTCT GGCTCAATAC AGGGACTGTG CATTGGGTTC AGGCTATTGG CTGGTGCAAC 
AACATTGCCT GGAACGTAGG ACCACTCACT GTGCATCAGT ACAAGCTAGC GGTGGAGCGC 
TATGAGTGGA ACAAACTCCA GAGTGTCAAA TCCATCGTTC CCATGATTCA CCTCTCCTGG 
AATATGGCCA GAAACATCAA AGTGTCAGAC CACAAGCTCT TTGAGATGAT CAAGTACTGT 
TTATTGAGGA CACTAAAGCA GTGTCAGATG CTGAGGGAGG CTCTCATAGC TGCTGGAAAA 
GAGCTTGTTT GGCATGGAAG GACCAAGGAC GAACCTGCTC ACTACTGTAG CATCTGTGAG 
GTGGAGGTCT TTGATCTGCT GTTTGTCACC AGTGAAAGTA ACTCAAGAAA GACGTATGTG 
GTTCACTGCC AGGGCTGCGC TCGCAGATGT AGCCCTAACC TGGACAACTT TGTGGTTTTA 
GAACAGTACA AAATAGACGA CCTCATGCAA GTGTACGACC AGTTCACATT AGCTCCACCT 
CTGCCCTCCT CCTCCTCATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm6a ( XM_005167798.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167855.1 Danio rerio lysine (K)-specific demethylase 6A (kdm6a), transcript variant X4, mRNA.

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CloneID	ODa15362
Clone ID Related Accession (Same CDS sequence)	XM_005167798.3 , XM_005167798.4
Accession Version	XM_005167798.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	lysine-specific demethylase 6A isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167798.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTTTC AGCTGGCATT GATTGACTCC ACACCCTGCA CTTTGTCCAA AGCTGAAATT 
CAGTTCCACA TTGCTCATGT ATATGAAATT CAGAAGAAAT ACCGCATCGC AAAGGAAGCT 
TACGAGAGTC TGTTACAAAC AGAAAATCTC CCTGCACAGG TGAAGGCAAC TACGCTCCAA 
CAATTAGGCT GGATGCATCA CACGGTCGAA CAGTTGGGAG ATAAAGCAAA CAAGAACAGC 
TATGCTATTC AGTGTCTGCA GAAATCCCTA GAAGCAGACC CCAATTCTGG ACAGTCTTGG 
TACTTCCTGG GCAGGTGCTA CTCAAGTATT GGGAAGGTTC AAGATGCCTT CATATCTTAC 
CGACAGTCTA TTGATAAGTC TGAGGCCAGT GCAGATACAT GGTGCTCAAT CGGTGTGTTG 
TATCAGCAGC AGAACCAGCC GATGGACGCA CTGCAGGCAT ATATCTGTGC AGTACAGCTG 
GACCACAGCC ATGCGGCTGC CTGGATGGAC CTGGGCACGC TCTACGAGTC CTGTAACCAG 
CCACAGGATG CCATCAAGTG CTACATCAAT GCCACGTGCA GCAAGTCCTG CAGTAACATC 
CCAGCTCTAA CTGCTCGCAT CAAATGCCTC CAGGCTCAGT TGTGTAATCT TCAGCAAGGT 
AGTTTACCAA GTAAAAGTAA AATGCTCCCT AGTATTGAGG AGGCGTGGAG CCTACCAATC 
CCAGCAGAGC TCACCTCCAG ACAGGGAGCG CTCAACACGA CCCAACAGCA AACCACCTGC 
AAAGCTCATC AGAGCAATGA AGCCCAGTCT CAAATCCCAA GCCAGTCTCT TCCTCCTCAT 
GTGCTCTCTG GCCAGACAGA AGACCAGTCC AGCCCGGCCA AGAAAAAGAG GACCACTAGT 
CCAGCCAGGA ATTCATCAGA TTCCTGGACA AATGATCCAG GTCATCATCA GATCTCCAAC 
TGGAGTTTAA CCCCACAGAA ACTTCAGCTC CTGGAACAGT TACGGAACAA CAGAGCCAAT 
CTGAAGCCAG TGCAGCTGCA GATGCTGGAA CAGTTGGAAA AGCAACTGAG TCTAATGCAA 
CAGAATCAAC AGACAAGGCT GAATGCCATG GGTCAGATAC GACCCAGTGT GTCCAACGGT 
CCTGTAGCCG ACTCTTCACT GCCTACAAAC TCTAATTCCA CACCTTCCCC TCACCATCCA 
CACATCGCTC TGTCTCGCAC ACCCTCTGCC CTCACACGAT GCGCTGCTCA GCCAATAGCC 
AACGGACCTG TCCCTGCGAG CCCCGTCCCA TGCAGCACCG CTGGTACCCT GGGTAACACA 
GATGCCGTCT CTGTGGGCAA TAATCATCTC CCAGGATCGG GGAGCAACGG AAATGTGCCT 
TACTTGCGGC AAAACGCACT ACCTCATAAC TGCACAACCC CCACCAGCAG CAGCATGGAT 
GATGAACAGT GGAAAAGCCA ACACATCAAC TCCACTCAGG GGCTTCAGAA AAGTCCAGGT 
TCATATTCAG CAGGTCCTAA CGGCGAGCAG CCTTTCTCTT CCACTGGGAC GTCCCAGCCT 
TCCCCGGCAG CCGGCACTGG CGGTCCAAAT CAGGTCGGAC ATTCGGCCAC AGCCAGCGAC 
ACGCAAGCGC AGGAGGCCAG TCACAATCAC CTCCCCTCCC CGAGCTCCCA GAGCTCTGCT 
ACCTCAGGTG GACAGCAAGG CACGGTGAAC ACCAAAGAGA GCAAGCCTTC AGGAAACGGG 
CATTCCGTGG GGACGCCTAA CAGCACTGCC ACTGCAGAGG GACTGCCTAA TCATGTCCAT 
CAGGGCCAGG CAGACACTGC TGGGGCGAAA CCCCGTTCAC CAGGTGTACT TAGTTCAGAC 
AATCCACAGC TCTCGGCCTT GTTGATTGGA AAAGCCAATG ATAGTAATAA TAGTAATGGA 
GGAGGGTCGG CGGCTGCTGG CACAAAGGTT AACAATGTTC ACCCGAGTCT GCACAAGCCG 
CAGGAAAACT CGGTGGCTTC TTCTCCATGT TCTGCCATGT CTGCAGCTTC GCCCTCACCC 
AAAACTGCAG ACCACCACAG TACTCAGAAC AGTGTTAACA GCCTTAACAG CCCCACGCTC 
AACGGCAAAG GTCTGGAGGA CTCTCAGAGC CCCTTGAAGG TGGCTTCTCC ACTGGTTTGT 
CATAAACCAA CGCCTCCGTC GTTCACTCTG TGGTCCTCTG TGTCCATCTA CCCCAGCTCC 
AGTGATGTGC TCAAAGCATG CAGAAACCTG GGGAAGAATG GTGTGTCCAA TAACAGCATC 
TTACTAGACA AGTGTCCCCC TCCACGGTTA CCACCTCCAC CCATCCCTCC TCTGCCAAAG 
GACAAACTCA ATCCTCCAAC CCCTAGTATT TATTTGGAGA ACAAGAGGGA TGCTTTCTTT 
CCACCGCTGC ACCAGTTCTG CACAAACACA GCCAACCCTG TAACAGTGAT CCGTGGCCTG 
GCTGGAGCAC TCAAGCTAGA CCTTGGATTA TTCTCCACCA AGACTCTGGT TGAGGCTAAC 
CCAGAGCACC TTGTGGAGGT GAGGACTCAG TTGTCTCAGC CCACCGATGA GAACTGGGAT 
GTGACAGGGA GCAGGAAGAT GTGGCGGTGT GAGAGCAGTC GGTCTCACAC CACCATTGCC 
AGATATGCTC AATACCAGGC CTCATCTTTC CAGGAATCAT TGCGGGAGGA AAATGAAAAG 
AAAGGACAGA AAGACCACTC GGATACGGAG TCTGCTCCAT CAGAAAATGT GGTGCGGAGA 
AGAAGAGGTC CCTTTAAGCA CATCAAATTT GGAACCAACA TTGATTTGTC AGATGAGAAA 
AAGTGGAAGC TGCAGCTGGC TGAGTTGAGT AAACTGCCTG CCTTCGCACG GGTGGTGTCT 
GCAGGGAATC TGCTCAGTCA TGTGGGCCAC ACAATTCTCG GCATGAACAC AGTCCAGCTC 
TATATGAAGG TCCCGGGCAG CAGGACACCA GGTCATCAAG AAAACAACAA CTTCTGCTCT 
GTGAACATAA ACATTGGCCC TGGAGATTGT GAATGGTTTG CCGTTCCTGA GCCGTACTGG 
GGTGTCATGA ATGATTTCTG TGAAAAGAAT AATATTAATT ATCTAATGGG ATCTTGGTGG 
CCAAATCTTG AGGACTTGTA TGAGGCAAAT GTTCCAGTGT ATCGCTTTAT CCAGCGACCA 
GGTGATCTGG TCTGGCTCAA TACAGGGACT GTGCATTGGG TTCAGGCTAT TGGCTGGTGC 
AACAACATTG CCTGGAACGT AGGACCACTC ACTGTGCATC AGTACAAGCT AGCGGTGGAG 
CGCTATGAGT GGAACAAACT CCAGAGTGTC AAATCCATCG TTCCCATGAT TCACCTCTCC 
TGGAATATGG CCAGAAACAT CAAAGTGTCA GACCACAAGC TCTTTGAGAT GATCAAGTAC 
TGTTTATTGA GGACACTAAA GCAGTGTCAG ATGCTGAGGG AGGCTCTCAT AGCTGCTGGA 
AAAGAGCTTG TTTGGCATGG AAGGACCAAG GACGAACCTG CTCACTACTG TAGCATCTGT 
GAGGTGGAGG TCTTTGATCT GCTGTTTGTC ACCAGTGAAA GTAACTCAAG AAAGACGTAT 
GTGGTTCACT GCCAGGGCTG CGCTCGCAGA TGTAGCCCTA ACCTGGACAA CTTTGTGGTT 
TTAGAACAGT ACAAAATAGA CGACCTCATG CAAGTGTACG ACCAGTTCAC ATTAGCTCCA 
CCTCTGCCCT CCTCCTCCTC ATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167855.1
CDS	92..3838
Translation	MHFQLALIDSTPCTLSKAEIQFHIAHVYEIQKKYRIAKEAYESLLQTENLPAQVKATTLQQLGWMHHTVEQLGDKANKNSYAIQCLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHSHAAAWMDLGTLYESCNQPQDAIKCYINATCSKSCSNIPALTARIKCLQAQLCNLQQGSLPSKSKMLPSIEEAWSLPIPAELTSRQGALNTTQQQTTCKAHQSNEAQSQIPSQSLPPHVLSGQTEDQSSPAKKKRTTSPARNSSDSWTNDPGHHQISNWSLTPQKLQLLEQLRNNRANLKPVQLQMLEQLEKQLSLMQQNQQTRLNAMGQIRPSVSNGPVADSSLPTNSNSTPSPHHPHIALSRTPSALTRCAAQPIANGPVPASPVPCSTAGTLGNTDAVSVGNNHLPGSGSNGNVPYLRQNALPHNCTTPTSSSMDDEQWKSQHINSTQGLQKSPGSYSAGPNGEQPFSSTGTSQPSPAAGTGGPNQVGHSATASDTQAQEASHNHLPSPSSQSSATSGGQQGTVNTKESKPSGNGHSVGTPNSTATAEGLPNHVHQGQADTAGAKPRSPGVLSSDNPQLSALLIGKANDSNNSNGGGSAAAGTKVNNVHPSLHKPQENSVASSPCSAMSAASPSPKTADHHSTQNSVNSLNSPTLNGKGLEDSQSPLKVASPLVCHKPTPPSFTLWSSVSIYPSSSDVLKACRNLGKNGVSNNSILLDKCPPPRLPPPPIPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNTANPVTVIRGLAGALKLDLGLFSTKTLVEANPEHLVEVRTQLSQPTDENWDVTGSRKMWRCESSRSHTTIARYAQYQASSFQESLREENEKKGQKDHSDTESAPSENVVRRRRGPFKHIKFGTNIDLSDEKKWKLQLAELSKLPAFARVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVPEPYWGVMNDFCEKNNINYLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWLNTGTVHWVQAIGWCNNIAWNVGPLTVHQYKLAVERYEWNKLQSVKSIVPMIHLSWNMARNIKVSDHKLFEMIKYCLLRTLKQCQMLREALIAAGKELVWHGRTKDEPAHYCSICEVEVFDLLFVTSESNSRKTYVVHCQGCARRCSPNLDNFVVLEQYKIDDLMQVYDQFTLAPPLPSSSSS

Target ORF information:

RefSeq Version	XM_005167798.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 6A (kdm6a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167798.3

ORF Insert Sequence:

ATGCATTTTC AGCTGGCATT GATTGACTCC ACACCCTGCA CTTTGTCCAA AGCTGAAATT 
CAGTTCCACA TTGCTCATGT ATATGAAATT CAGAAGAAAT ACCGCATCGC AAAGGAAGCT 
TACGAGAGTC TGTTACAAAC AGAAAATCTC CCTGCACAGG TGAAGGCAAC TACGCTCCAA 
CAATTAGGCT GGATGCATCA CACGGTCGAA CAGTTGGGAG ATAAAGCAAA CAAGAACAGC 
TATGCTATTC AGTGTCTGCA GAAATCCCTA GAAGCAGACC CCAATTCTGG ACAGTCTTGG 
TACTTCCTGG GCAGGTGCTA CTCAAGTATT GGGAAGGTTC AAGATGCCTT CATATCTTAC 
CGACAGTCTA TTGATAAGTC TGAGGCCAGT GCAGATACAT GGTGCTCAAT CGGTGTGTTG 
TATCAGCAGC AGAACCAGCC GATGGACGCA CTGCAGGCAT ATATCTGTGC AGTACAGCTG 
GACCACAGCC ATGCGGCTGC CTGGATGGAC CTGGGCACGC TCTACGAGTC CTGTAACCAG 
CCACAGGATG CCATCAAGTG CTACATCAAT GCCACGTGCA GCAAGTCCTG CAGTAACATC 
CCAGCTCTAA CTGCTCGCAT CAAATGCCTC CAGGCTCAGT TGTGTAATCT TCAGCAAGGT 
AGTTTACCAA GTAAAAGTAA AATGCTCCCT AGTATTGAGG AGGCGTGGAG CCTACCAATC 
CCAGCAGAGC TCACCTCCAG ACAGGGAGCG CTCAACACGA CCCAACAGCA AACCACCTGC 
AAAGCTCATC AGAGCAATGA AGCCCAGTCT CAAATCCCAA GCCAGTCTCT TCCTCCTCAT 
GTGCTCTCTG GCCAGACAGA AGACCAGTCC AGCCCGGCCA AGAAAAAGAG GACCACTAGT 
CCAGCCAGGA ATTCATCAGA TTCCTGGACA AATGATCCAG GTCATCATCA GATCTCCAAC 
TGGAGTTTAA CCCCACAGAA ACTTCAGCTC CTGGAACAGT TACGGAACAA CAGAGCCAAT 
CTGAAGCCAG TGCAGCTGCA GATGCTGGAA CAGTTGGAAA AGCAACTGAG TCTAATGCAA 
CAGAATCAAC AGACAAGGCT GAATGCCATG GGTCAGATAC GACCCAGTGT GTCCAACGGT 
CCTGTAGCCG ACTCTTCACT GCCTACAAAC TCTAATTCCA CACCTTCCCC TCACCATCCA 
CACATCGCTC TGTCTCGCAC ACCCTCTGCC CTCACACGAT GCGCTGCTCA GCCAATAGCC 
AACGGACCTG TCCCTGCGAG CCCCGTCCCA TGCAGCACCG CTGGTACCCT GGGTAACACA 
GATGCCGTCT CTGTGGGCAA TAATCATCTC CCAGGATCGG GGAGCAACGG AAATGTGCCT 
TACTTGCGGC AAAACGCACT ACCTCATAAC TGCACAACCC CCACCAGCAG CAGCATGGAT 
GATGAACAGT GGAAAAGCCA ACACATCAAC TCCACTCAGG GGCTTCAGAA AAGTCCAGGT 
TCATATTCAG CAGGTCCTAA CGGCGAGCAG CCTTTCTCTT CCACTGGGAC GTCCCAGCCT 
TCCCCGGCAG CCGGCACTGG CGGTCCAAAT CAGGTCGGAC ATTCGGCCAC AGCCAGCGAC 
ACGCAAGCGC AGGAGGCCAG TCACAATCAC CTCCCCTCCC CGAGCTCCCA GAGCTCTGCT 
ACCTCAGGTG GACAGCAAGG CACGGTGAAC ACCAAAGAGA GCAAGCCTTC AGGAAACGGG 
CATTCCGTGG GGACGCCTAA CAGCACTGCC ACTGCAGAGG GACTGCCTAA TCATGTCCAT 
CAGGGCCAGG CAGACACTGC TGGGGCGAAA CCCCGTTCAC CAGGTGTACT TAGTTCAGAC 
AATCCACAGC TCTCGGCCTT GTTGATTGGA AAAGCCAATG ATAGTAATAA TAGTAATGGA 
GGAGGGTCGG CGGCTGCTGG CACAAAGGTT AACAATGTTC ACCCGAGTCT GCACAAGCCG 
CAGGAAAACT CGGTGGCTTC TTCTCCATGT TCTGCCATGT CTGCAGCTTC GCCCTCACCC 
AAAACTGCAG ACCACCACAG TACTCAGAAC AGTGTTAACA GCCTTAACAG CCCCACGCTC 
AACGGCAAAG GTCTGGAGGA CTCTCAGAGC CCCTTGAAGG TGGCTTCTCC ACTGGTTTGT 
CATAAACCAA CGCCTCCGTC GTTCACTCTG TGGTCCTCTG TGTCCATCTA CCCCAGCTCC 
AGTGATGTGC TCAAAGCATG CAGAAACCTG GGGAAGAATG GTGTGTCCAA TAACAGCATC 
TTACTAGACA AGTGTCCCCC TCCACGGTTA CCACCTCCAC CCATCCCTCC TCTGCCAAAG 
GACAAACTCA ATCCTCCAAC CCCTAGTATT TATTTGGAGA ACAAGAGGGA TGCTTTCTTT 
CCACCGCTGC ACCAGTTCTG CACAAACACA GCCAACCCTG TAACAGTGAT CCGTGGCCTG 
GCTGGAGCAC TCAAGCTAGA CCTTGGATTA TTCTCCACCA AGACTCTGGT TGAGGCTAAC 
CCAGAGCACC TTGTGGAGGT GAGGACTCAG TTGTCTCAGC CCACCGATGA GAACTGGGAT 
GTGACAGGGA GCAGGAAGAT GTGGCGGTGT GAGAGCAGTC GGTCTCACAC CACCATTGCC 
AGATATGCTC AATACCAGGC CTCATCTTTC CAGGAATCAT TGCGGGAGGA AAATGAAAAG 
AAAGGACAGA AAGACCACTC GGATACGGAG TCTGCTCCAT CAGAAAATGT GGTGCGGAGA 
AGAAGAGGTC CCTTTAAGCA CATCAAATTT GGAACCAACA TTGATTTGTC AGATGAGAAA 
AAGTGGAAGC TGCAGCTGGC TGAGTTGAGT AAACTGCCTG CCTTCGCACG GGTGGTGTCT 
GCAGGGAATC TGCTCAGTCA TGTGGGCCAC ACAATTCTCG GCATGAACAC AGTCCAGCTC 
TATATGAAGG TCCCGGGCAG CAGGACACCA GGTCATCAAG AAAACAACAA CTTCTGCTCT 
GTGAACATAA ACATTGGCCC TGGAGATTGT GAATGGTTTG CCGTTCCTGA GCCGTACTGG 
GGTGTCATGA ATGATTTCTG TGAAAAGAAT AATATTAATT ATCTAATGGG ATCTTGGTGG 
CCAAATCTTG AGGACTTGTA TGAGGCAAAT GTTCCAGTGT ATCGCTTTAT CCAGCGACCA 
GGTGATCTGG TCTGGCTCAA TACAGGGACT GTGCATTGGG TTCAGGCTAT TGGCTGGTGC 
AACAACATTG CCTGGAACGT AGGACCACTC ACTGTGCATC AGTACAAGCT AGCGGTGGAG 
CGCTATGAGT GGAACAAACT CCAGAGTGTC AAATCCATCG TTCCCATGAT TCACCTCTCC 
TGGAATATGG CCAGAAACAT CAAAGTGTCA GACCACAAGC TCTTTGAGAT GATCAAGTAC 
TGTTTATTGA GGACACTAAA GCAGTGTCAG ATGCTGAGGG AGGCTCTCAT AGCTGCTGGA 
AAAGAGCTTG TTTGGCATGG AAGGACCAAG GACGAACCTG CTCACTACTG TAGCATCTGT 
GAGGTGGAGG TCTTTGATCT GCTGTTTGTC ACCAGTGAAA GTAACTCAAG AAAGACGTAT 
GTGGTTCACT GCCAGGGCTG CGCTCGCAGA TGTAGCCCTA ACCTGGACAA CTTTGTGGTT 
TTAGAACAGT ACAAAATAGA CGACCTCATG CAAGTGTACG ACCAGTTCAC ATTAGCTCCA 
CCTCTGCCCT CCTCCTCCTC ATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm6a ( XM_005167798.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167855.1 Danio rerio lysine (K)-specific demethylase 6A (kdm6a), transcript variant X4, mRNA.

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CloneID	ODa15362
Clone ID Related Accession (Same CDS sequence)	XM_005167798.3 , XM_005167798.4
Accession Version	XM_005167798.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3747bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	lysine-specific demethylase 6A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167798.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCATTTTC AGCTGGCATT GATTGACTCC ACACCCTGCA CTTTGTCCAA AGCTGAAATT 
CAGTTCCACA TTGCTCATGT ATATGAAATT CAGAAGAAAT ACCGCATCGC AAAGGAAGCT 
TACGAGAGTC TGTTACAAAC AGAAAATCTC CCTGCACAGG TGAAGGCAAC TACGCTCCAA 
CAATTAGGCT GGATGCATCA CACGGTCGAA CAGTTGGGAG ATAAAGCAAA CAAGAACAGC 
TATGCTATTC AGTGTCTGCA GAAATCCCTA GAAGCAGACC CCAATTCTGG ACAGTCTTGG 
TACTTCCTGG GCAGGTGCTA CTCAAGTATT GGGAAGGTTC AAGATGCCTT CATATCTTAC 
CGACAGTCTA TTGATAAGTC TGAGGCCAGT GCAGATACAT GGTGCTCAAT CGGTGTGTTG 
TATCAGCAGC AGAACCAGCC GATGGACGCA CTGCAGGCAT ATATCTGTGC AGTACAGCTG 
GACCACAGCC ATGCGGCTGC CTGGATGGAC CTGGGCACGC TCTACGAGTC CTGTAACCAG 
CCACAGGATG CCATCAAGTG CTACATCAAT GCCACGTGCA GCAAGTCCTG CAGTAACATC 
CCAGCTCTAA CTGCTCGCAT CAAATGCCTC CAGGCTCAGT TGTGTAATCT TCAGCAAGGT 
AGTTTACCAA GTAAAAGTAA AATGCTCCCT AGTATTGAGG AGGCGTGGAG CCTACCAATC 
CCAGCAGAGC TCACCTCCAG ACAGGGAGCG CTCAACACGA CCCAACAGCA AACCACCTGC 
AAAGCTCATC AGAGCAATGA AGCCCAGTCT CAAATCCCAA GCCAGTCTCT TCCTCCTCAT 
GTGCTCTCTG GCCAGACAGA AGACCAGTCC AGCCCGGCCA AGAAAAAGAG GACCACTAGT 
CCAGCCAGGA ATTCATCAGA TTCCTGGACA AATGATCCAG GTCATCATCA GATCTCCAAC 
TGGAGTTTAA CCCCACAGAA ACTTCAGCTC CTGGAACAGT TACGGAACAA CAGAGCCAAT 
CTGAAGCCAG TGCAGCTGCA GATGCTGGAA CAGTTGGAAA AGCAACTGAG TCTAATGCAA 
CAGAATCAAC AGACAAGGCT GAATGCCATG GGTCAGATAC GACCCAGTGT GTCCAACGGT 
CCTGTAGCCG ACTCTTCACT GCCTACAAAC TCTAATTCCA CACCTTCCCC TCACCATCCA 
CACATCGCTC TGTCTCGCAC ACCCTCTGCC CTCACACGAT GCGCTGCTCA GCCAATAGCC 
AACGGACCTG TCCCTGCGAG CCCCGTCCCA TGCAGCACCG CTGGTACCCT GGGTAACACA 
GATGCCGTCT CTGTGGGCAA TAATCATCTC CCAGGATCGG GGAGCAACGG AAATGTGCCT 
TACTTGCGGC AAAACGCACT ACCTCATAAC TGCACAACCC CCACCAGCAG CAGCATGGAT 
GATGAACAGT GGAAAAGCCA ACACATCAAC TCCACTCAGG GGCTTCAGAA AAGTCCAGGT 
TCATATTCAG CAGGTCCTAA CGGCGAGCAG CCTTTCTCTT CCACTGGGAC GTCCCAGCCT 
TCCCCGGCAG CCGGCACTGG CGGTCCAAAT CAGGTCGGAC ATTCGGCCAC AGCCAGCGAC 
ACGCAAGCGC AGGAGGCCAG TCACAATCAC CTCCCCTCCC CGAGCTCCCA GAGCTCTGCT 
ACCTCAGGTG GACAGCAAGG CACGGTGAAC ACCAAAGAGA GCAAGCCTTC AGGAAACGGG 
CATTCCGTGG GGACGCCTAA CAGCACTGCC ACTGCAGAGG GACTGCCTAA TCATGTCCAT 
CAGGGCCAGG CAGACACTGC TGGGGCGAAA CCCCGTTCAC CAGGTGTACT TAGTTCAGAC 
AATCCACAGC TCTCGGCCTT GTTGATTGGA AAAGCCAATG ATAGTAATAA TAGTAATGGA 
GGAGGGTCGG CGGCTGCTGG CACAAAGGTT AACAATGTTC ACCCGAGTCT GCACAAGCCG 
CAGGAAAACT CGGTGGCTTC TTCTCCATGT TCTGCCATGT CTGCAGCTTC GCCCTCACCC 
AAAACTGCAG ACCACCACAG TACTCAGAAC AGTGTTAACA GCCTTAACAG CCCCACGCTC 
AACGGCAAAG GTCTGGAGGA CTCTCAGAGC CCCTTGAAGG TGGCTTCTCC ACTGGTTTGT 
CATAAACCAA CGCCTCCGTC GTTCACTCTG TGGTCCTCTG TGTCCATCTA CCCCAGCTCC 
AGTGATGTGC TCAAAGCATG CAGAAACCTG GGGAAGAATG GTGTGTCCAA TAACAGCATC 
TTACTAGACA AGTGTCCCCC TCCACGGTTA CCACCTCCAC CCATCCCTCC TCTGCCAAAG 
GACAAACTCA ATCCTCCAAC CCCTAGTATT TATTTGGAGA ACAAGAGGGA TGCTTTCTTT 
CCACCGCTGC ACCAGTTCTG CACAAACACA GCCAACCCTG TAACAGTGAT CCGTGGCCTG 
GCTGGAGCAC TCAAGCTAGA CCTTGGATTA TTCTCCACCA AGACTCTGGT TGAGGCTAAC 
CCAGAGCACC TTGTGGAGGT GAGGACTCAG TTGTCTCAGC CCACCGATGA GAACTGGGAT 
GTGACAGGGA GCAGGAAGAT GTGGCGGTGT GAGAGCAGTC GGTCTCACAC CACCATTGCC 
AGATATGCTC AATACCAGGC CTCATCTTTC CAGGAATCAT TGCGGGAGGA AAATGAAAAG 
AAAGGACAGA AAGACCACTC GGATACGGAG TCTGCTCCAT CAGAAAATGT GGTGCGGAGA 
AGAAGAGGTC CCTTTAAGCA CATCAAATTT GGAACCAACA TTGATTTGTC AGATGAGAAA 
AAGTGGAAGC TGCAGCTGGC TGAGTTGAGT AAACTGCCTG CCTTCGCACG GGTGGTGTCT 
GCAGGGAATC TGCTCAGTCA TGTGGGCCAC ACAATTCTCG GCATGAACAC AGTCCAGCTC 
TATATGAAGG TCCCGGGCAG CAGGACACCA GGTCATCAAG AAAACAACAA CTTCTGCTCT 
GTGAACATAA ACATTGGCCC TGGAGATTGT GAATGGTTTG CCGTTCCTGA GCCGTACTGG 
GGTGTCATGA ATGATTTCTG TGAAAAGAAT AATATTAATT ATCTAATGGG ATCTTGGTGG 
CCAAATCTTG AGGACTTGTA TGAGGCAAAT GTTCCAGTGT ATCGCTTTAT CCAGCGACCA 
GGTGATCTGG TCTGGCTCAA TACAGGGACT GTGCATTGGG TTCAGGCTAT TGGCTGGTGC 
AACAACATTG CCTGGAACGT AGGACCACTC ACTGTGCATC AGTACAAGCT AGCGGTGGAG 
CGCTATGAGT GGAACAAACT CCAGAGTGTC AAATCCATCG TTCCCATGAT TCACCTCTCC 
TGGAATATGG CCAGAAACAT CAAAGTGTCA GACCACAAGC TCTTTGAGAT GATCAAGTAC 
TGTTTATTGA GGACACTAAA GCAGTGTCAG ATGCTGAGGG AGGCTCTCAT AGCTGCTGGA 
AAAGAGCTTG TTTGGCATGG AAGGACCAAG GACGAACCTG CTCACTACTG TAGCATCTGT 
GAGGTGGAGG TCTTTGATCT GCTGTTTGTC ACCAGTGAAA GTAACTCAAG AAAGACGTAT 
GTGGTTCACT GCCAGGGCTG CGCTCGCAGA TGTAGCCCTA ACCTGGACAA CTTTGTGGTT 
TTAGAACAGT ACAAAATAGA CGACCTCATG CAAGTGTACG ACCAGTTCAC ATTAGCTCCA 
CCTCTGCCCT CCTCCTCCTC ATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167855.1
CDS	99..3845
Translation	MHFQLALIDSTPCTLSKAEIQFHIAHVYEIQKKYRIAKEAYESLLQTENLPAQVKATTLQQLGWMHHTVEQLGDKANKNSYAIQCLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHSHAAAWMDLGTLYESCNQPQDAIKCYINATCSKSCSNIPALTARIKCLQAQLCNLQQGSLPSKSKMLPSIEEAWSLPIPAELTSRQGALNTTQQQTTCKAHQSNEAQSQIPSQSLPPHVLSGQTEDQSSPAKKKRTTSPARNSSDSWTNDPGHHQISNWSLTPQKLQLLEQLRNNRANLKPVQLQMLEQLEKQLSLMQQNQQTRLNAMGQIRPSVSNGPVADSSLPTNSNSTPSPHHPHIALSRTPSALTRCAAQPIANGPVPASPVPCSTAGTLGNTDAVSVGNNHLPGSGSNGNVPYLRQNALPHNCTTPTSSSMDDEQWKSQHINSTQGLQKSPGSYSAGPNGEQPFSSTGTSQPSPAAGTGGPNQVGHSATASDTQAQEASHNHLPSPSSQSSATSGGQQGTVNTKESKPSGNGHSVGTPNSTATAEGLPNHVHQGQADTAGAKPRSPGVLSSDNPQLSALLIGKANDSNNSNGGGSAAAGTKVNNVHPSLHKPQENSVASSPCSAMSAASPSPKTADHHSTQNSVNSLNSPTLNGKGLEDSQSPLKVASPLVCHKPTPPSFTLWSSVSIYPSSSDVLKACRNLGKNGVSNNSILLDKCPPPRLPPPPIPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNTANPVTVIRGLAGALKLDLGLFSTKTLVEANPEHLVEVRTQLSQPTDENWDVTGSRKMWRCESSRSHTTIARYAQYQASSFQESLREENEKKGQKDHSDTESAPSENVVRRRRGPFKHIKFGTNIDLSDEKKWKLQLAELSKLPAFARVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVPEPYWGVMNDFCEKNNINYLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWLNTGTVHWVQAIGWCNNIAWNVGPLTVHQYKLAVERYEWNKLQSVKSIVPMIHLSWNMARNIKVSDHKLFEMIKYCLLRTLKQCQMLREALIAAGKELVWHGRTKDEPAHYCSICEVEVFDLLFVTSESNSRKTYVVHCQGCARRCSPNLDNFVVLEQYKIDDLMQVYDQFTLAPPLPSSSSS

Target ORF information:

RefSeq Version	XM_005167798.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 6A (kdm6a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167798.4

ORF Insert Sequence:

ATGCATTTTC AGCTGGCATT GATTGACTCC ACACCCTGCA CTTTGTCCAA AGCTGAAATT 
CAGTTCCACA TTGCTCATGT ATATGAAATT CAGAAGAAAT ACCGCATCGC AAAGGAAGCT 
TACGAGAGTC TGTTACAAAC AGAAAATCTC CCTGCACAGG TGAAGGCAAC TACGCTCCAA 
CAATTAGGCT GGATGCATCA CACGGTCGAA CAGTTGGGAG ATAAAGCAAA CAAGAACAGC 
TATGCTATTC AGTGTCTGCA GAAATCCCTA GAAGCAGACC CCAATTCTGG ACAGTCTTGG 
TACTTCCTGG GCAGGTGCTA CTCAAGTATT GGGAAGGTTC AAGATGCCTT CATATCTTAC 
CGACAGTCTA TTGATAAGTC TGAGGCCAGT GCAGATACAT GGTGCTCAAT CGGTGTGTTG 
TATCAGCAGC AGAACCAGCC GATGGACGCA CTGCAGGCAT ATATCTGTGC AGTACAGCTG 
GACCACAGCC ATGCGGCTGC CTGGATGGAC CTGGGCACGC TCTACGAGTC CTGTAACCAG 
CCACAGGATG CCATCAAGTG CTACATCAAT GCCACGTGCA GCAAGTCCTG CAGTAACATC 
CCAGCTCTAA CTGCTCGCAT CAAATGCCTC CAGGCTCAGT TGTGTAATCT TCAGCAAGGT 
AGTTTACCAA GTAAAAGTAA AATGCTCCCT AGTATTGAGG AGGCGTGGAG CCTACCAATC 
CCAGCAGAGC TCACCTCCAG ACAGGGAGCG CTCAACACGA CCCAACAGCA AACCACCTGC 
AAAGCTCATC AGAGCAATGA AGCCCAGTCT CAAATCCCAA GCCAGTCTCT TCCTCCTCAT 
GTGCTCTCTG GCCAGACAGA AGACCAGTCC AGCCCGGCCA AGAAAAAGAG GACCACTAGT 
CCAGCCAGGA ATTCATCAGA TTCCTGGACA AATGATCCAG GTCATCATCA GATCTCCAAC 
TGGAGTTTAA CCCCACAGAA ACTTCAGCTC CTGGAACAGT TACGGAACAA CAGAGCCAAT 
CTGAAGCCAG TGCAGCTGCA GATGCTGGAA CAGTTGGAAA AGCAACTGAG TCTAATGCAA 
CAGAATCAAC AGACAAGGCT GAATGCCATG GGTCAGATAC GACCCAGTGT GTCCAACGGT 
CCTGTAGCCG ACTCTTCACT GCCTACAAAC TCTAATTCCA CACCTTCCCC TCACCATCCA 
CACATCGCTC TGTCTCGCAC ACCCTCTGCC CTCACACGAT GCGCTGCTCA GCCAATAGCC 
AACGGACCTG TCCCTGCGAG CCCCGTCCCA TGCAGCACCG CTGGTACCCT GGGTAACACA 
GATGCCGTCT CTGTGGGCAA TAATCATCTC CCAGGATCGG GGAGCAACGG AAATGTGCCT 
TACTTGCGGC AAAACGCACT ACCTCATAAC TGCACAACCC CCACCAGCAG CAGCATGGAT 
GATGAACAGT GGAAAAGCCA ACACATCAAC TCCACTCAGG GGCTTCAGAA AAGTCCAGGT 
TCATATTCAG CAGGTCCTAA CGGCGAGCAG CCTTTCTCTT CCACTGGGAC GTCCCAGCCT 
TCCCCGGCAG CCGGCACTGG CGGTCCAAAT CAGGTCGGAC ATTCGGCCAC AGCCAGCGAC 
ACGCAAGCGC AGGAGGCCAG TCACAATCAC CTCCCCTCCC CGAGCTCCCA GAGCTCTGCT 
ACCTCAGGTG GACAGCAAGG CACGGTGAAC ACCAAAGAGA GCAAGCCTTC AGGAAACGGG 
CATTCCGTGG GGACGCCTAA CAGCACTGCC ACTGCAGAGG GACTGCCTAA TCATGTCCAT 
CAGGGCCAGG CAGACACTGC TGGGGCGAAA CCCCGTTCAC CAGGTGTACT TAGTTCAGAC 
AATCCACAGC TCTCGGCCTT GTTGATTGGA AAAGCCAATG ATAGTAATAA TAGTAATGGA 
GGAGGGTCGG CGGCTGCTGG CACAAAGGTT AACAATGTTC ACCCGAGTCT GCACAAGCCG 
CAGGAAAACT CGGTGGCTTC TTCTCCATGT TCTGCCATGT CTGCAGCTTC GCCCTCACCC 
AAAACTGCAG ACCACCACAG TACTCAGAAC AGTGTTAACA GCCTTAACAG CCCCACGCTC 
AACGGCAAAG GTCTGGAGGA CTCTCAGAGC CCCTTGAAGG TGGCTTCTCC ACTGGTTTGT 
CATAAACCAA CGCCTCCGTC GTTCACTCTG TGGTCCTCTG TGTCCATCTA CCCCAGCTCC 
AGTGATGTGC TCAAAGCATG CAGAAACCTG GGGAAGAATG GTGTGTCCAA TAACAGCATC 
TTACTAGACA AGTGTCCCCC TCCACGGTTA CCACCTCCAC CCATCCCTCC TCTGCCAAAG 
GACAAACTCA ATCCTCCAAC CCCTAGTATT TATTTGGAGA ACAAGAGGGA TGCTTTCTTT 
CCACCGCTGC ACCAGTTCTG CACAAACACA GCCAACCCTG TAACAGTGAT CCGTGGCCTG 
GCTGGAGCAC TCAAGCTAGA CCTTGGATTA TTCTCCACCA AGACTCTGGT TGAGGCTAAC 
CCAGAGCACC TTGTGGAGGT GAGGACTCAG TTGTCTCAGC CCACCGATGA GAACTGGGAT 
GTGACAGGGA GCAGGAAGAT GTGGCGGTGT GAGAGCAGTC GGTCTCACAC CACCATTGCC 
AGATATGCTC AATACCAGGC CTCATCTTTC CAGGAATCAT TGCGGGAGGA AAATGAAAAG 
AAAGGACAGA AAGACCACTC GGATACGGAG TCTGCTCCAT CAGAAAATGT GGTGCGGAGA 
AGAAGAGGTC CCTTTAAGCA CATCAAATTT GGAACCAACA TTGATTTGTC AGATGAGAAA 
AAGTGGAAGC TGCAGCTGGC TGAGTTGAGT AAACTGCCTG CCTTCGCACG GGTGGTGTCT 
GCAGGGAATC TGCTCAGTCA TGTGGGCCAC ACAATTCTCG GCATGAACAC AGTCCAGCTC 
TATATGAAGG TCCCGGGCAG CAGGACACCA GGTCATCAAG AAAACAACAA CTTCTGCTCT 
GTGAACATAA ACATTGGCCC TGGAGATTGT GAATGGTTTG CCGTTCCTGA GCCGTACTGG 
GGTGTCATGA ATGATTTCTG TGAAAAGAAT AATATTAATT ATCTAATGGG ATCTTGGTGG 
CCAAATCTTG AGGACTTGTA TGAGGCAAAT GTTCCAGTGT ATCGCTTTAT CCAGCGACCA 
GGTGATCTGG TCTGGCTCAA TACAGGGACT GTGCATTGGG TTCAGGCTAT TGGCTGGTGC 
AACAACATTG CCTGGAACGT AGGACCACTC ACTGTGCATC AGTACAAGCT AGCGGTGGAG 
CGCTATGAGT GGAACAAACT CCAGAGTGTC AAATCCATCG TTCCCATGAT TCACCTCTCC 
TGGAATATGG CCAGAAACAT CAAAGTGTCA GACCACAAGC TCTTTGAGAT GATCAAGTAC 
TGTTTATTGA GGACACTAAA GCAGTGTCAG ATGCTGAGGG AGGCTCTCAT AGCTGCTGGA 
AAAGAGCTTG TTTGGCATGG AAGGACCAAG GACGAACCTG CTCACTACTG TAGCATCTGT 
GAGGTGGAGG TCTTTGATCT GCTGTTTGTC ACCAGTGAAA GTAACTCAAG AAAGACGTAT 
GTGGTTCACT GCCAGGGCTG CGCTCGCAGA TGTAGCCCTA ACCTGGACAA CTTTGTGGTT 
TTAGAACAGT ACAAAATAGA CGACCTCATG CAAGTGTACG ACCAGTTCAC ATTAGCTCCA 
CCTCTGCCCT CCTCCTCCTC ATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm6a ( XM_005167794.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167851.1 Danio rerio lysine (K)-specific demethylase 6A (kdm6a), transcript variant X1, mRNA.

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CloneID	ODa15357
Clone ID Related Accession (Same CDS sequence)	XM_005167794.4 , XM_005167794.3
Accession Version	XM_005167794.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	lysine-specific demethylase 6A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167794.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCGT GCGGAGTGTC GGTGGCCGCT GTAGCTCGCG CTGCTGCTGC TGCTGCTGCT 
GGTGGTGATG AGGAAAAGAA AATGGCGGCG GGAAAAGCGA GAGAAACCGA GGAGGACTTT 
CCGAAGCTTA CGCCGCAAGA AAGGGAGCGC TTAGCCAGCA TAGACAGCAC ACTGTTTGGA 
TTTCAGAGGC TTCATGAAGA TGGCGCCAGA ACGAAGGCCT TGCTGTTAAA GGCTGTTCGC 
TGCTATGACG TCTATATTTT AAAAGCTGAA GGGAAAGTGG AGCCAGAGGT GTTTTGCCAG 
TTAGGTCACT TCAACCTTTT ACTGGAAGAT TATCCTAAAG CATTATCTGC ATACCAGAGA 
TACTACAGTT TACAGTCAGA CTACTGGAAG AATGCTGCCT TTTTATATGG CCTTGGTTTG 
GTTTACTTCC ATTACAATGC TTTTCGATGG GCAGTGCAGC TCACCCCCTC AGATGCCCAA 
TCTGGACAGA GAAGGGCGAT CAAAGCATTT CAGGAGGTGC TTTACATTGA CCCCTGCTTC 
TCAAGAGCCA AGGAGATCCA TCTGCGACTC GGTCTGATGT TTAAAGTCAA CACGGACTAT 
GAGTCAAGCC TCAAGCATTT TCAGCTGGCA TTGATTGACT CCACACCCTG CACTTTGTCC 
AAAGCTGAAA TTCAGTTCCA CATTGCTCAT GTATATGAAA TTCAGAAGAA ATACCGCATC 
GCAAAGGAAG CTTACGAGAG TCTGTTACAA ACAGAAAATC TCCCTGCACA GGTGAAGGCA 
ACTACGCTCC AACAATTAGG CTGGATGCAT CACACGGTCG AACAGTTGGG AGATAAAGCA 
AACAAGAACA GCTATGCTAT TCAGTGTCTG CAGAAATCCC TAGAAGCAGA CCCCAATTCT 
GGACAGTCTT GGTACTTCCT GGGCAGGTGC TACTCAAGTA TTGGGAAGGT TCAAGATGCC 
TTCATATCTT ACCGACAGTC TATTGATAAG TCTGAGGCCA GTGCAGATAC ATGGTGCTCA 
ATCGGTGTGT TGTATCAGCA GCAGAACCAG CCGATGGACG CACTGCAGGC ATATATCTGT 
GCAGTACAGC TGGACCACAG CCATGCGGCT GCCTGGATGG ACCTGGGCAC GCTCTACGAG 
TCCTGTAACC AGCCACAGGA TGCCATCAAG TGCTACATCA ATGCCACGTG CAGCAAGTCC 
TGCAGTAACA TCCCAGCTCT AACTGCTCGC ATCAAATGCC TCCAGGCTCA GTTGTGTAAT 
CTTCAGCAAG GTAGTTTACC AAGTAAAAGT AAAATGCTCC CTAGTATTGA GGAGGCGTGG 
AGCCTACCAA TCCCAGCAGA GCTCACCTCC AGACAGGGAG CGCTCAACAC GACCCAACAG 
CAAACCACCT GCAAAGCTCA TCAGAGCAAT GAAGCCCAGT CTCAAATCCC AAGCCAGTCT 
CTTCCTCCTC ATGTGCTCTC TGGCCAGACA GAAGACCAGT CCAGCCCGGC CAAGAAAAAG 
AGGACCACTA GTCCAGCCAG GAATTCATCA GATTCCTGGA CAAATGATCC AGGTCATCAT 
CAGATCTCCA ACTGGAGTTT AACCCCACAG AAACTTCAGC TCCTGGAACA GTTACGGAAC 
AACAGAGCCA ATCTGAAGCC AGTGCAGCTG CAGATGCTGG AACAGTTGGA AAAGCAACTG 
AGTCTAATGC AACAGAATCA ACAGACAAGG CTGAATGCCA TGGGTCAGAT ACGACCCAGT 
GTGTCCAACG GTCCTGTAGC CGACTCTTCA CTGCCTACAA ACTCTAATTC CACACCTTCC 
CCTCACCATC CACACATCGC TCTGTCTCGC ACACCCTCTG CCCTCACACG ATGCGCTGCT 
CAGCCAATAG CCAACGGACC TGTCCCTGCG AGCCCCGTCC CATGCAGCAC CGCTGGTACC 
CTGGGTAACA CAGATGCCGT CTCTGTGGGC AATAATCATC TCCCAGGATC GGGGAGCAAC 
GGAAATGTGC CTTACTTGCG GCAAAACGCA CTACCTCATA ACTGCACAAC CCCCACCAGC 
AGCAGCATGG ATGATGAACA GTGGAAAAGC CAACACATCA ACTCCACTCA GGGGCTTCAG 
AAAAGTCCAG GTTCATATTC AGCAGGTCCT AACGGCGAGC AGCCTTTCTC TTCCACTGGG 
ACGTCCCAGC CTTCCCCGGC AGCCGGCACT GGCGGTCCAA ATCAGGTCGG ACATTCGGCC 
ACAGCCAGCG ACACGCAAGC GCAGGAGGCC AGTCACAATC ACCTCCCCTC CCCGAGCTCC 
CAGAGCTCTG CTACCTCAGG TGGACAGCAA GGCACGGTGA ACACCAAAGA GAGCAAGCCT 
TCAGGAAACG GGCATTCCGT GGGGACGCCT AACAGCACTG CCACTGCAGA GGGACTGCCT 
AATCATGTCC ATCAGGGCCA GGCAGACACT GCTGGGGCGA AACCCCGTTC ACCAGGTGTA 
CTTAGTTCAG ACAATCCACA GCTCTCGGCC TTGTTGATTG GAAAAGCCAA TGATAGTAAT 
AATAGTAATG GAGGAGGGTC GGCGGCTGCT GGCACAAAGG TTAACAATGT TCACCCGAGT 
CTGCACAAGC CGCAGGAAAA CTCGGTGGCT TCTTCTCCAT GTTCTGCCAT GTCTGCAGCT 
TCGCCCTCAC CCAAAACTGC AGACCACCAC AGTACTCAGA ACAGTGTTAA CAGCCTTAAC 
AGCCCCACGC TCAACGGCAA AGGTCTGGAG GACTCTCAGA GCCCCTTGAA GGTGGCTTCT 
CCACTGGTTT GTCATAAACC AACGCCTCCG TCGTTCACTC TGTGGTCCTC TGTGTCCATC 
TACCCCAGCT CCAGTGATGT GCTCAAAGCA TGCAGAAACC TGGGGAAGAA TGGTGTGTCC 
AATAACAGCA TCTTACTAGA CAAGTGTCCC CCTCCACGGT TACCACCTCC ACCCATCCCT 
CCTCTGCCAA AGGACAAACT CAATCCTCCA ACCCCTAGTA TTTATTTGGA GAACAAGAGG 
GATGCTTTCT TTCCACCGCT GCACCAGTTC TGCACAAACA CAGCCAACCC TGTAACAGTG 
ATCCGTGGCC TGGCTGGAGC ACTCAAGCTA GACCTTGGAT TATTCTCCAC CAAGACTCTG 
GTTGAGGCTA ACCCAGAGCA CCTTGTGGAG GTGAGGACTC AGTTGTCTCA GCCCACCGAT 
GAGAACTGGG ATGTGACAGG GAGCAGGAAG ATGTGGCGGT GTGAGAGCAG TCGGTCTCAC 
ACCACCATTG CCAGATATGC TCAATACCAG GCCTCATCTT TCCAGGAATC ATTGCGGGAG 
GAAAATGAAA AGAAAGGACA GAAAGACCAC TCGGATACGG AGTCTGCTCC ATCAGAAAAT 
GTGGTGCGGA GAAGAAGAGG TCCCTTTAAG CACATCAAAT TTGGAACCAA CATTGATTTG 
TCAGATGAGA AAAAGTGGAA GCTGCAGCTG GCTGAGTTGA GTAAACTGCC TGCCTTCGCA 
CGGGTGGTGT CTGCAGGGAA TCTGCTCAGT CATGTGGGCC ACACAATTCT CGGCATGAAC 
ACAGTCCAGC TCTATATGAA GGTCCCGGGC AGCAGGACAC CAGGTCATCA AGAAAACAAC 
AACTTCTGCT CTGTGAACAT AAACATTGGC CCTGGAGATT GTGAATGGTT TGCCGTTCCT 
GAGCCGTACT GGGGTGTCAT GAATGATTTC TGTGAAAAGA ATAATATTAA TTATCTAATG 
GGATCTTGGT GGCCAAATCT TGAGGACTTG TATGAGGCAA ATGTTCCAGT GTATCGCTTT 
ATCCAGCGAC CAGGTGATCT GGTCTGGCTC AATACAGGGA CTGTGCATTG GGTTCAGGCT 
ATTGGCTGGT GCAACAACAT TGCCTGGAAC GTAGGACCAC TCACTGTGCA TCAGTACAAG 
CTAGCGGTGG AGCGCTATGA GTGGAACAAA CTCCAGAGTG TCAAATCCAT CGTTCCCATG 
ATTCACCTCT CCTGGAATAT GGCCAGAAAC ATCAAAGTGT CAGACCACAA GCTCTTTGAG 
ATGATCAAGT ACTGTTTATT GAGGACACTA AAGCAGTGTC AGATGCTGAG GGAGGCTCTC 
ATAGCTGCTG GAAAAGAGCT TGTTTGGCAT GGAAGGACCA AGGACGAACC TGCTCACTAC 
TGTAGCATCT GTGAGGTGGA GGTCTTTGAT CTGCTGTTTG TCACCAGTGA AAGTAACTCA 
AGAAAGACGT ATGTGGTTCA CTGCCAGGGC TGCGCTCGCA GATGTAGCCC TAACCTGGAC 
AACTTTGTGG TTTTAGAACA GTACAAAATA GACGACCTCA TGCAAGTGTA CGACCAGTTC 
ACATTAGCTC CACCTCTGCC CTCCTCCTCC TCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167851.1
CDS	469..4827
Translation	MKSCGVSVAAVARAAAAAAAGGDEEKKMAAGKARETEEDFPKLTPQERERLASIDSTLFGFQRLHEDGARTKALLLKAVRCYDVYILKAEGKVEPEVFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFRWAVQLTPSDAQSGQRRAIKAFQEVLYIDPCFSRAKEIHLRLGLMFKVNTDYESSLKHFQLALIDSTPCTLSKAEIQFHIAHVYEIQKKYRIAKEAYESLLQTENLPAQVKATTLQQLGWMHHTVEQLGDKANKNSYAIQCLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHSHAAAWMDLGTLYESCNQPQDAIKCYINATCSKSCSNIPALTARIKCLQAQLCNLQQGSLPSKSKMLPSIEEAWSLPIPAELTSRQGALNTTQQQTTCKAHQSNEAQSQIPSQSLPPHVLSGQTEDQSSPAKKKRTTSPARNSSDSWTNDPGHHQISNWSLTPQKLQLLEQLRNNRANLKPVQLQMLEQLEKQLSLMQQNQQTRLNAMGQIRPSVSNGPVADSSLPTNSNSTPSPHHPHIALSRTPSALTRCAAQPIANGPVPASPVPCSTAGTLGNTDAVSVGNNHLPGSGSNGNVPYLRQNALPHNCTTPTSSSMDDEQWKSQHINSTQGLQKSPGSYSAGPNGEQPFSSTGTSQPSPAAGTGGPNQVGHSATASDTQAQEASHNHLPSPSSQSSATSGGQQGTVNTKESKPSGNGHSVGTPNSTATAEGLPNHVHQGQADTAGAKPRSPGVLSSDNPQLSALLIGKANDSNNSNGGGSAAAGTKVNNVHPSLHKPQENSVASSPCSAMSAASPSPKTADHHSTQNSVNSLNSPTLNGKGLEDSQSPLKVASPLVCHKPTPPSFTLWSSVSIYPSSSDVLKACRNLGKNGVSNNSILLDKCPPPRLPPPPIPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNTANPVTVIRGLAGALKLDLGLFSTKTLVEANPEHLVEVRTQLSQPTDENWDVTGSRKMWRCESSRSHTTIARYAQYQASSFQESLREENEKKGQKDHSDTESAPSENVVRRRRGPFKHIKFGTNIDLSDEKKWKLQLAELSKLPAFARVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVPEPYWGVMNDFCEKNNINYLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWLNTGTVHWVQAIGWCNNIAWNVGPLTVHQYKLAVERYEWNKLQSVKSIVPMIHLSWNMARNIKVSDHKLFEMIKYCLLRTLKQCQMLREALIAAGKELVWHGRTKDEPAHYCSICEVEVFDLLFVTSESNSRKTYVVHCQGCARRCSPNLDNFVVLEQYKIDDLMQVYDQFTLAPPLPSSSSS

Target ORF information:

RefSeq Version	XM_005167794.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 6A (kdm6a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167794.4

ORF Insert Sequence:

ATGAAATCGT GCGGAGTGTC GGTGGCCGCT GTAGCTCGCG CTGCTGCTGC TGCTGCTGCT 
GGTGGTGATG AGGAAAAGAA AATGGCGGCG GGAAAAGCGA GAGAAACCGA GGAGGACTTT 
CCGAAGCTTA CGCCGCAAGA AAGGGAGCGC TTAGCCAGCA TAGACAGCAC ACTGTTTGGA 
TTTCAGAGGC TTCATGAAGA TGGCGCCAGA ACGAAGGCCT TGCTGTTAAA GGCTGTTCGC 
TGCTATGACG TCTATATTTT AAAAGCTGAA GGGAAAGTGG AGCCAGAGGT GTTTTGCCAG 
TTAGGTCACT TCAACCTTTT ACTGGAAGAT TATCCTAAAG CATTATCTGC ATACCAGAGA 
TACTACAGTT TACAGTCAGA CTACTGGAAG AATGCTGCCT TTTTATATGG CCTTGGTTTG 
GTTTACTTCC ATTACAATGC TTTTCGATGG GCAGTGCAGC TCACCCCCTC AGATGCCCAA 
TCTGGACAGA GAAGGGCGAT CAAAGCATTT CAGGAGGTGC TTTACATTGA CCCCTGCTTC 
TCAAGAGCCA AGGAGATCCA TCTGCGACTC GGTCTGATGT TTAAAGTCAA CACGGACTAT 
GAGTCAAGCC TCAAGCATTT TCAGCTGGCA TTGATTGACT CCACACCCTG CACTTTGTCC 
AAAGCTGAAA TTCAGTTCCA CATTGCTCAT GTATATGAAA TTCAGAAGAA ATACCGCATC 
GCAAAGGAAG CTTACGAGAG TCTGTTACAA ACAGAAAATC TCCCTGCACA GGTGAAGGCA 
ACTACGCTCC AACAATTAGG CTGGATGCAT CACACGGTCG AACAGTTGGG AGATAAAGCA 
AACAAGAACA GCTATGCTAT TCAGTGTCTG CAGAAATCCC TAGAAGCAGA CCCCAATTCT 
GGACAGTCTT GGTACTTCCT GGGCAGGTGC TACTCAAGTA TTGGGAAGGT TCAAGATGCC 
TTCATATCTT ACCGACAGTC TATTGATAAG TCTGAGGCCA GTGCAGATAC ATGGTGCTCA 
ATCGGTGTGT TGTATCAGCA GCAGAACCAG CCGATGGACG CACTGCAGGC ATATATCTGT 
GCAGTACAGC TGGACCACAG CCATGCGGCT GCCTGGATGG ACCTGGGCAC GCTCTACGAG 
TCCTGTAACC AGCCACAGGA TGCCATCAAG TGCTACATCA ATGCCACGTG CAGCAAGTCC 
TGCAGTAACA TCCCAGCTCT AACTGCTCGC ATCAAATGCC TCCAGGCTCA GTTGTGTAAT 
CTTCAGCAAG GTAGTTTACC AAGTAAAAGT AAAATGCTCC CTAGTATTGA GGAGGCGTGG 
AGCCTACCAA TCCCAGCAGA GCTCACCTCC AGACAGGGAG CGCTCAACAC GACCCAACAG 
CAAACCACCT GCAAAGCTCA TCAGAGCAAT GAAGCCCAGT CTCAAATCCC AAGCCAGTCT 
CTTCCTCCTC ATGTGCTCTC TGGCCAGACA GAAGACCAGT CCAGCCCGGC CAAGAAAAAG 
AGGACCACTA GTCCAGCCAG GAATTCATCA GATTCCTGGA CAAATGATCC AGGTCATCAT 
CAGATCTCCA ACTGGAGTTT AACCCCACAG AAACTTCAGC TCCTGGAACA GTTACGGAAC 
AACAGAGCCA ATCTGAAGCC AGTGCAGCTG CAGATGCTGG AACAGTTGGA AAAGCAACTG 
AGTCTAATGC AACAGAATCA ACAGACAAGG CTGAATGCCA TGGGTCAGAT ACGACCCAGT 
GTGTCCAACG GTCCTGTAGC CGACTCTTCA CTGCCTACAA ACTCTAATTC CACACCTTCC 
CCTCACCATC CACACATCGC TCTGTCTCGC ACACCCTCTG CCCTCACACG ATGCGCTGCT 
CAGCCAATAG CCAACGGACC TGTCCCTGCG AGCCCCGTCC CATGCAGCAC CGCTGGTACC 
CTGGGTAACA CAGATGCCGT CTCTGTGGGC AATAATCATC TCCCAGGATC GGGGAGCAAC 
GGAAATGTGC CTTACTTGCG GCAAAACGCA CTACCTCATA ACTGCACAAC CCCCACCAGC 
AGCAGCATGG ATGATGAACA GTGGAAAAGC CAACACATCA ACTCCACTCA GGGGCTTCAG 
AAAAGTCCAG GTTCATATTC AGCAGGTCCT AACGGCGAGC AGCCTTTCTC TTCCACTGGG 
ACGTCCCAGC CTTCCCCGGC AGCCGGCACT GGCGGTCCAA ATCAGGTCGG ACATTCGGCC 
ACAGCCAGCG ACACGCAAGC GCAGGAGGCC AGTCACAATC ACCTCCCCTC CCCGAGCTCC 
CAGAGCTCTG CTACCTCAGG TGGACAGCAA GGCACGGTGA ACACCAAAGA GAGCAAGCCT 
TCAGGAAACG GGCATTCCGT GGGGACGCCT AACAGCACTG CCACTGCAGA GGGACTGCCT 
AATCATGTCC ATCAGGGCCA GGCAGACACT GCTGGGGCGA AACCCCGTTC ACCAGGTGTA 
CTTAGTTCAG ACAATCCACA GCTCTCGGCC TTGTTGATTG GAAAAGCCAA TGATAGTAAT 
AATAGTAATG GAGGAGGGTC GGCGGCTGCT GGCACAAAGG TTAACAATGT TCACCCGAGT 
CTGCACAAGC CGCAGGAAAA CTCGGTGGCT TCTTCTCCAT GTTCTGCCAT GTCTGCAGCT 
TCGCCCTCAC CCAAAACTGC AGACCACCAC AGTACTCAGA ACAGTGTTAA CAGCCTTAAC 
AGCCCCACGC TCAACGGCAA AGGTCTGGAG GACTCTCAGA GCCCCTTGAA GGTGGCTTCT 
CCACTGGTTT GTCATAAACC AACGCCTCCG TCGTTCACTC TGTGGTCCTC TGTGTCCATC 
TACCCCAGCT CCAGTGATGT GCTCAAAGCA TGCAGAAACC TGGGGAAGAA TGGTGTGTCC 
AATAACAGCA TCTTACTAGA CAAGTGTCCC CCTCCACGGT TACCACCTCC ACCCATCCCT 
CCTCTGCCAA AGGACAAACT CAATCCTCCA ACCCCTAGTA TTTATTTGGA GAACAAGAGG 
GATGCTTTCT TTCCACCGCT GCACCAGTTC TGCACAAACA CAGCCAACCC TGTAACAGTG 
ATCCGTGGCC TGGCTGGAGC ACTCAAGCTA GACCTTGGAT TATTCTCCAC CAAGACTCTG 
GTTGAGGCTA ACCCAGAGCA CCTTGTGGAG GTGAGGACTC AGTTGTCTCA GCCCACCGAT 
GAGAACTGGG ATGTGACAGG GAGCAGGAAG ATGTGGCGGT GTGAGAGCAG TCGGTCTCAC 
ACCACCATTG CCAGATATGC TCAATACCAG GCCTCATCTT TCCAGGAATC ATTGCGGGAG 
GAAAATGAAA AGAAAGGACA GAAAGACCAC TCGGATACGG AGTCTGCTCC ATCAGAAAAT 
GTGGTGCGGA GAAGAAGAGG TCCCTTTAAG CACATCAAAT TTGGAACCAA CATTGATTTG 
TCAGATGAGA AAAAGTGGAA GCTGCAGCTG GCTGAGTTGA GTAAACTGCC TGCCTTCGCA 
CGGGTGGTGT CTGCAGGGAA TCTGCTCAGT CATGTGGGCC ACACAATTCT CGGCATGAAC 
ACAGTCCAGC TCTATATGAA GGTCCCGGGC AGCAGGACAC CAGGTCATCA AGAAAACAAC 
AACTTCTGCT CTGTGAACAT AAACATTGGC CCTGGAGATT GTGAATGGTT TGCCGTTCCT 
GAGCCGTACT GGGGTGTCAT GAATGATTTC TGTGAAAAGA ATAATATTAA TTATCTAATG 
GGATCTTGGT GGCCAAATCT TGAGGACTTG TATGAGGCAA ATGTTCCAGT GTATCGCTTT 
ATCCAGCGAC CAGGTGATCT GGTCTGGCTC AATACAGGGA CTGTGCATTG GGTTCAGGCT 
ATTGGCTGGT GCAACAACAT TGCCTGGAAC GTAGGACCAC TCACTGTGCA TCAGTACAAG 
CTAGCGGTGG AGCGCTATGA GTGGAACAAA CTCCAGAGTG TCAAATCCAT CGTTCCCATG 
ATTCACCTCT CCTGGAATAT GGCCAGAAAC ATCAAAGTGT CAGACCACAA GCTCTTTGAG 
ATGATCAAGT ACTGTTTATT GAGGACACTA AAGCAGTGTC AGATGCTGAG GGAGGCTCTC 
ATAGCTGCTG GAAAAGAGCT TGTTTGGCAT GGAAGGACCA AGGACGAACC TGCTCACTAC 
TGTAGCATCT GTGAGGTGGA GGTCTTTGAT CTGCTGTTTG TCACCAGTGA AAGTAACTCA 
AGAAAGACGT ATGTGGTTCA CTGCCAGGGC TGCGCTCGCA GATGTAGCCC TAACCTGGAC 
AACTTTGTGG TTTTAGAACA GTACAAAATA GACGACCTCA TGCAAGTGTA CGACCAGTTC 
ACATTAGCTC CACCTCTGCC CTCCTCCTCC TCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm6a ( XM_005167794.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167851.1 Danio rerio lysine (K)-specific demethylase 6A (kdm6a), transcript variant X1, mRNA.

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CloneID	ODa15357
Clone ID Related Accession (Same CDS sequence)	XM_005167794.4 , XM_005167794.3
Accession Version	XM_005167794.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	lysine-specific demethylase 6A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167794.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCGT GCGGAGTGTC GGTGGCCGCT GTAGCTCGCG CTGCTGCTGC TGCTGCTGCT 
GGTGGTGATG AGGAAAAGAA AATGGCGGCG GGAAAAGCGA GAGAAACCGA GGAGGACTTT 
CCGAAGCTTA CGCCGCAAGA AAGGGAGCGC TTAGCCAGCA TAGACAGCAC ACTGTTTGGA 
TTTCAGAGGC TTCATGAAGA TGGCGCCAGA ACGAAGGCCT TGCTGTTAAA GGCTGTTCGC 
TGCTATGACG TCTATATTTT AAAAGCTGAA GGGAAAGTGG AGCCAGAGGT GTTTTGCCAG 
TTAGGTCACT TCAACCTTTT ACTGGAAGAT TATCCTAAAG CATTATCTGC ATACCAGAGA 
TACTACAGTT TACAGTCAGA CTACTGGAAG AATGCTGCCT TTTTATATGG CCTTGGTTTG 
GTTTACTTCC ATTACAATGC TTTTCGATGG GCAGTGCAGC TCACCCCCTC AGATGCCCAA 
TCTGGACAGA GAAGGGCGAT CAAAGCATTT CAGGAGGTGC TTTACATTGA CCCCTGCTTC 
TCAAGAGCCA AGGAGATCCA TCTGCGACTC GGTCTGATGT TTAAAGTCAA CACGGACTAT 
GAGTCAAGCC TCAAGCATTT TCAGCTGGCA TTGATTGACT CCACACCCTG CACTTTGTCC 
AAAGCTGAAA TTCAGTTCCA CATTGCTCAT GTATATGAAA TTCAGAAGAA ATACCGCATC 
GCAAAGGAAG CTTACGAGAG TCTGTTACAA ACAGAAAATC TCCCTGCACA GGTGAAGGCA 
ACTACGCTCC AACAATTAGG CTGGATGCAT CACACGGTCG AACAGTTGGG AGATAAAGCA 
AACAAGAACA GCTATGCTAT TCAGTGTCTG CAGAAATCCC TAGAAGCAGA CCCCAATTCT 
GGACAGTCTT GGTACTTCCT GGGCAGGTGC TACTCAAGTA TTGGGAAGGT TCAAGATGCC 
TTCATATCTT ACCGACAGTC TATTGATAAG TCTGAGGCCA GTGCAGATAC ATGGTGCTCA 
ATCGGTGTGT TGTATCAGCA GCAGAACCAG CCGATGGACG CACTGCAGGC ATATATCTGT 
GCAGTACAGC TGGACCACAG CCATGCGGCT GCCTGGATGG ACCTGGGCAC GCTCTACGAG 
TCCTGTAACC AGCCACAGGA TGCCATCAAG TGCTACATCA ATGCCACGTG CAGCAAGTCC 
TGCAGTAACA TCCCAGCTCT AACTGCTCGC ATCAAATGCC TCCAGGCTCA GTTGTGTAAT 
CTTCAGCAAG GTAGTTTACC AAGTAAAAGT AAAATGCTCC CTAGTATTGA GGAGGCGTGG 
AGCCTACCAA TCCCAGCAGA GCTCACCTCC AGACAGGGAG CGCTCAACAC GACCCAACAG 
CAAACCACCT GCAAAGCTCA TCAGAGCAAT GAAGCCCAGT CTCAAATCCC AAGCCAGTCT 
CTTCCTCCTC ATGTGCTCTC TGGCCAGACA GAAGACCAGT CCAGCCCGGC CAAGAAAAAG 
AGGACCACTA GTCCAGCCAG GAATTCATCA GATTCCTGGA CAAATGATCC AGGTCATCAT 
CAGATCTCCA ACTGGAGTTT AACCCCACAG AAACTTCAGC TCCTGGAACA GTTACGGAAC 
AACAGAGCCA ATCTGAAGCC AGTGCAGCTG CAGATGCTGG AACAGTTGGA AAAGCAACTG 
AGTCTAATGC AACAGAATCA ACAGACAAGG CTGAATGCCA TGGGTCAGAT ACGACCCAGT 
GTGTCCAACG GTCCTGTAGC CGACTCTTCA CTGCCTACAA ACTCTAATTC CACACCTTCC 
CCTCACCATC CACACATCGC TCTGTCTCGC ACACCCTCTG CCCTCACACG ATGCGCTGCT 
CAGCCAATAG CCAACGGACC TGTCCCTGCG AGCCCCGTCC CATGCAGCAC CGCTGGTACC 
CTGGGTAACA CAGATGCCGT CTCTGTGGGC AATAATCATC TCCCAGGATC GGGGAGCAAC 
GGAAATGTGC CTTACTTGCG GCAAAACGCA CTACCTCATA ACTGCACAAC CCCCACCAGC 
AGCAGCATGG ATGATGAACA GTGGAAAAGC CAACACATCA ACTCCACTCA GGGGCTTCAG 
AAAAGTCCAG GTTCATATTC AGCAGGTCCT AACGGCGAGC AGCCTTTCTC TTCCACTGGG 
ACGTCCCAGC CTTCCCCGGC AGCCGGCACT GGCGGTCCAA ATCAGGTCGG ACATTCGGCC 
ACAGCCAGCG ACACGCAAGC GCAGGAGGCC AGTCACAATC ACCTCCCCTC CCCGAGCTCC 
CAGAGCTCTG CTACCTCAGG TGGACAGCAA GGCACGGTGA ACACCAAAGA GAGCAAGCCT 
TCAGGAAACG GGCATTCCGT GGGGACGCCT AACAGCACTG CCACTGCAGA GGGACTGCCT 
AATCATGTCC ATCAGGGCCA GGCAGACACT GCTGGGGCGA AACCCCGTTC ACCAGGTGTA 
CTTAGTTCAG ACAATCCACA GCTCTCGGCC TTGTTGATTG GAAAAGCCAA TGATAGTAAT 
AATAGTAATG GAGGAGGGTC GGCGGCTGCT GGCACAAAGG TTAACAATGT TCACCCGAGT 
CTGCACAAGC CGCAGGAAAA CTCGGTGGCT TCTTCTCCAT GTTCTGCCAT GTCTGCAGCT 
TCGCCCTCAC CCAAAACTGC AGACCACCAC AGTACTCAGA ACAGTGTTAA CAGCCTTAAC 
AGCCCCACGC TCAACGGCAA AGGTCTGGAG GACTCTCAGA GCCCCTTGAA GGTGGCTTCT 
CCACTGGTTT GTCATAAACC AACGCCTCCG TCGTTCACTC TGTGGTCCTC TGTGTCCATC 
TACCCCAGCT CCAGTGATGT GCTCAAAGCA TGCAGAAACC TGGGGAAGAA TGGTGTGTCC 
AATAACAGCA TCTTACTAGA CAAGTGTCCC CCTCCACGGT TACCACCTCC ACCCATCCCT 
CCTCTGCCAA AGGACAAACT CAATCCTCCA ACCCCTAGTA TTTATTTGGA GAACAAGAGG 
GATGCTTTCT TTCCACCGCT GCACCAGTTC TGCACAAACA CAGCCAACCC TGTAACAGTG 
ATCCGTGGCC TGGCTGGAGC ACTCAAGCTA GACCTTGGAT TATTCTCCAC CAAGACTCTG 
GTTGAGGCTA ACCCAGAGCA CCTTGTGGAG GTGAGGACTC AGTTGTCTCA GCCCACCGAT 
GAGAACTGGG ATGTGACAGG GAGCAGGAAG ATGTGGCGGT GTGAGAGCAG TCGGTCTCAC 
ACCACCATTG CCAGATATGC TCAATACCAG GCCTCATCTT TCCAGGAATC ATTGCGGGAG 
GAAAATGAAA AGAAAGGACA GAAAGACCAC TCGGATACGG AGTCTGCTCC ATCAGAAAAT 
GTGGTGCGGA GAAGAAGAGG TCCCTTTAAG CACATCAAAT TTGGAACCAA CATTGATTTG 
TCAGATGAGA AAAAGTGGAA GCTGCAGCTG GCTGAGTTGA GTAAACTGCC TGCCTTCGCA 
CGGGTGGTGT CTGCAGGGAA TCTGCTCAGT CATGTGGGCC ACACAATTCT CGGCATGAAC 
ACAGTCCAGC TCTATATGAA GGTCCCGGGC AGCAGGACAC CAGGTCATCA AGAAAACAAC 
AACTTCTGCT CTGTGAACAT AAACATTGGC CCTGGAGATT GTGAATGGTT TGCCGTTCCT 
GAGCCGTACT GGGGTGTCAT GAATGATTTC TGTGAAAAGA ATAATATTAA TTATCTAATG 
GGATCTTGGT GGCCAAATCT TGAGGACTTG TATGAGGCAA ATGTTCCAGT GTATCGCTTT 
ATCCAGCGAC CAGGTGATCT GGTCTGGCTC AATACAGGGA CTGTGCATTG GGTTCAGGCT 
ATTGGCTGGT GCAACAACAT TGCCTGGAAC GTAGGACCAC TCACTGTGCA TCAGTACAAG 
CTAGCGGTGG AGCGCTATGA GTGGAACAAA CTCCAGAGTG TCAAATCCAT CGTTCCCATG 
ATTCACCTCT CCTGGAATAT GGCCAGAAAC ATCAAAGTGT CAGACCACAA GCTCTTTGAG 
ATGATCAAGT ACTGTTTATT GAGGACACTA AAGCAGTGTC AGATGCTGAG GGAGGCTCTC 
ATAGCTGCTG GAAAAGAGCT TGTTTGGCAT GGAAGGACCA AGGACGAACC TGCTCACTAC 
TGTAGCATCT GTGAGGTGGA GGTCTTTGAT CTGCTGTTTG TCACCAGTGA AAGTAACTCA 
AGAAAGACGT ATGTGGTTCA CTGCCAGGGC TGCGCTCGCA GATGTAGCCC TAACCTGGAC 
AACTTTGTGG TTTTAGAACA GTACAAAATA GACGACCTCA TGCAAGTGTA CGACCAGTTC 
ACATTAGCTC CACCTCTGCC CTCCTCCTCC TCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167851.1
CDS	623..4981
Translation	MKSCGVSVAAVARAAAAAAAGGDEEKKMAAGKARETEEDFPKLTPQERERLASIDSTLFGFQRLHEDGARTKALLLKAVRCYDVYILKAEGKVEPEVFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFRWAVQLTPSDAQSGQRRAIKAFQEVLYIDPCFSRAKEIHLRLGLMFKVNTDYESSLKHFQLALIDSTPCTLSKAEIQFHIAHVYEIQKKYRIAKEAYESLLQTENLPAQVKATTLQQLGWMHHTVEQLGDKANKNSYAIQCLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHSHAAAWMDLGTLYESCNQPQDAIKCYINATCSKSCSNIPALTARIKCLQAQLCNLQQGSLPSKSKMLPSIEEAWSLPIPAELTSRQGALNTTQQQTTCKAHQSNEAQSQIPSQSLPPHVLSGQTEDQSSPAKKKRTTSPARNSSDSWTNDPGHHQISNWSLTPQKLQLLEQLRNNRANLKPVQLQMLEQLEKQLSLMQQNQQTRLNAMGQIRPSVSNGPVADSSLPTNSNSTPSPHHPHIALSRTPSALTRCAAQPIANGPVPASPVPCSTAGTLGNTDAVSVGNNHLPGSGSNGNVPYLRQNALPHNCTTPTSSSMDDEQWKSQHINSTQGLQKSPGSYSAGPNGEQPFSSTGTSQPSPAAGTGGPNQVGHSATASDTQAQEASHNHLPSPSSQSSATSGGQQGTVNTKESKPSGNGHSVGTPNSTATAEGLPNHVHQGQADTAGAKPRSPGVLSSDNPQLSALLIGKANDSNNSNGGGSAAAGTKVNNVHPSLHKPQENSVASSPCSAMSAASPSPKTADHHSTQNSVNSLNSPTLNGKGLEDSQSPLKVASPLVCHKPTPPSFTLWSSVSIYPSSSDVLKACRNLGKNGVSNNSILLDKCPPPRLPPPPIPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNTANPVTVIRGLAGALKLDLGLFSTKTLVEANPEHLVEVRTQLSQPTDENWDVTGSRKMWRCESSRSHTTIARYAQYQASSFQESLREENEKKGQKDHSDTESAPSENVVRRRRGPFKHIKFGTNIDLSDEKKWKLQLAELSKLPAFARVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVPEPYWGVMNDFCEKNNINYLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWLNTGTVHWVQAIGWCNNIAWNVGPLTVHQYKLAVERYEWNKLQSVKSIVPMIHLSWNMARNIKVSDHKLFEMIKYCLLRTLKQCQMLREALIAAGKELVWHGRTKDEPAHYCSICEVEVFDLLFVTSESNSRKTYVVHCQGCARRCSPNLDNFVVLEQYKIDDLMQVYDQFTLAPPLPSSSSS

Target ORF information:

RefSeq Version	XM_005167794.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 6A (kdm6a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167794.3

ORF Insert Sequence:

ATGAAATCGT GCGGAGTGTC GGTGGCCGCT GTAGCTCGCG CTGCTGCTGC TGCTGCTGCT 
GGTGGTGATG AGGAAAAGAA AATGGCGGCG GGAAAAGCGA GAGAAACCGA GGAGGACTTT 
CCGAAGCTTA CGCCGCAAGA AAGGGAGCGC TTAGCCAGCA TAGACAGCAC ACTGTTTGGA 
TTTCAGAGGC TTCATGAAGA TGGCGCCAGA ACGAAGGCCT TGCTGTTAAA GGCTGTTCGC 
TGCTATGACG TCTATATTTT AAAAGCTGAA GGGAAAGTGG AGCCAGAGGT GTTTTGCCAG 
TTAGGTCACT TCAACCTTTT ACTGGAAGAT TATCCTAAAG CATTATCTGC ATACCAGAGA 
TACTACAGTT TACAGTCAGA CTACTGGAAG AATGCTGCCT TTTTATATGG CCTTGGTTTG 
GTTTACTTCC ATTACAATGC TTTTCGATGG GCAGTGCAGC TCACCCCCTC AGATGCCCAA 
TCTGGACAGA GAAGGGCGAT CAAAGCATTT CAGGAGGTGC TTTACATTGA CCCCTGCTTC 
TCAAGAGCCA AGGAGATCCA TCTGCGACTC GGTCTGATGT TTAAAGTCAA CACGGACTAT 
GAGTCAAGCC TCAAGCATTT TCAGCTGGCA TTGATTGACT CCACACCCTG CACTTTGTCC 
AAAGCTGAAA TTCAGTTCCA CATTGCTCAT GTATATGAAA TTCAGAAGAA ATACCGCATC 
GCAAAGGAAG CTTACGAGAG TCTGTTACAA ACAGAAAATC TCCCTGCACA GGTGAAGGCA 
ACTACGCTCC AACAATTAGG CTGGATGCAT CACACGGTCG AACAGTTGGG AGATAAAGCA 
AACAAGAACA GCTATGCTAT TCAGTGTCTG CAGAAATCCC TAGAAGCAGA CCCCAATTCT 
GGACAGTCTT GGTACTTCCT GGGCAGGTGC TACTCAAGTA TTGGGAAGGT TCAAGATGCC 
TTCATATCTT ACCGACAGTC TATTGATAAG TCTGAGGCCA GTGCAGATAC ATGGTGCTCA 
ATCGGTGTGT TGTATCAGCA GCAGAACCAG CCGATGGACG CACTGCAGGC ATATATCTGT 
GCAGTACAGC TGGACCACAG CCATGCGGCT GCCTGGATGG ACCTGGGCAC GCTCTACGAG 
TCCTGTAACC AGCCACAGGA TGCCATCAAG TGCTACATCA ATGCCACGTG CAGCAAGTCC 
TGCAGTAACA TCCCAGCTCT AACTGCTCGC ATCAAATGCC TCCAGGCTCA GTTGTGTAAT 
CTTCAGCAAG GTAGTTTACC AAGTAAAAGT AAAATGCTCC CTAGTATTGA GGAGGCGTGG 
AGCCTACCAA TCCCAGCAGA GCTCACCTCC AGACAGGGAG CGCTCAACAC GACCCAACAG 
CAAACCACCT GCAAAGCTCA TCAGAGCAAT GAAGCCCAGT CTCAAATCCC AAGCCAGTCT 
CTTCCTCCTC ATGTGCTCTC TGGCCAGACA GAAGACCAGT CCAGCCCGGC CAAGAAAAAG 
AGGACCACTA GTCCAGCCAG GAATTCATCA GATTCCTGGA CAAATGATCC AGGTCATCAT 
CAGATCTCCA ACTGGAGTTT AACCCCACAG AAACTTCAGC TCCTGGAACA GTTACGGAAC 
AACAGAGCCA ATCTGAAGCC AGTGCAGCTG CAGATGCTGG AACAGTTGGA AAAGCAACTG 
AGTCTAATGC AACAGAATCA ACAGACAAGG CTGAATGCCA TGGGTCAGAT ACGACCCAGT 
GTGTCCAACG GTCCTGTAGC CGACTCTTCA CTGCCTACAA ACTCTAATTC CACACCTTCC 
CCTCACCATC CACACATCGC TCTGTCTCGC ACACCCTCTG CCCTCACACG ATGCGCTGCT 
CAGCCAATAG CCAACGGACC TGTCCCTGCG AGCCCCGTCC CATGCAGCAC CGCTGGTACC 
CTGGGTAACA CAGATGCCGT CTCTGTGGGC AATAATCATC TCCCAGGATC GGGGAGCAAC 
GGAAATGTGC CTTACTTGCG GCAAAACGCA CTACCTCATA ACTGCACAAC CCCCACCAGC 
AGCAGCATGG ATGATGAACA GTGGAAAAGC CAACACATCA ACTCCACTCA GGGGCTTCAG 
AAAAGTCCAG GTTCATATTC AGCAGGTCCT AACGGCGAGC AGCCTTTCTC TTCCACTGGG 
ACGTCCCAGC CTTCCCCGGC AGCCGGCACT GGCGGTCCAA ATCAGGTCGG ACATTCGGCC 
ACAGCCAGCG ACACGCAAGC GCAGGAGGCC AGTCACAATC ACCTCCCCTC CCCGAGCTCC 
CAGAGCTCTG CTACCTCAGG TGGACAGCAA GGCACGGTGA ACACCAAAGA GAGCAAGCCT 
TCAGGAAACG GGCATTCCGT GGGGACGCCT AACAGCACTG CCACTGCAGA GGGACTGCCT 
AATCATGTCC ATCAGGGCCA GGCAGACACT GCTGGGGCGA AACCCCGTTC ACCAGGTGTA 
CTTAGTTCAG ACAATCCACA GCTCTCGGCC TTGTTGATTG GAAAAGCCAA TGATAGTAAT 
AATAGTAATG GAGGAGGGTC GGCGGCTGCT GGCACAAAGG TTAACAATGT TCACCCGAGT 
CTGCACAAGC CGCAGGAAAA CTCGGTGGCT TCTTCTCCAT GTTCTGCCAT GTCTGCAGCT 
TCGCCCTCAC CCAAAACTGC AGACCACCAC AGTACTCAGA ACAGTGTTAA CAGCCTTAAC 
AGCCCCACGC TCAACGGCAA AGGTCTGGAG GACTCTCAGA GCCCCTTGAA GGTGGCTTCT 
CCACTGGTTT GTCATAAACC AACGCCTCCG TCGTTCACTC TGTGGTCCTC TGTGTCCATC 
TACCCCAGCT CCAGTGATGT GCTCAAAGCA TGCAGAAACC TGGGGAAGAA TGGTGTGTCC 
AATAACAGCA TCTTACTAGA CAAGTGTCCC CCTCCACGGT TACCACCTCC ACCCATCCCT 
CCTCTGCCAA AGGACAAACT CAATCCTCCA ACCCCTAGTA TTTATTTGGA GAACAAGAGG 
GATGCTTTCT TTCCACCGCT GCACCAGTTC TGCACAAACA CAGCCAACCC TGTAACAGTG 
ATCCGTGGCC TGGCTGGAGC ACTCAAGCTA GACCTTGGAT TATTCTCCAC CAAGACTCTG 
GTTGAGGCTA ACCCAGAGCA CCTTGTGGAG GTGAGGACTC AGTTGTCTCA GCCCACCGAT 
GAGAACTGGG ATGTGACAGG GAGCAGGAAG ATGTGGCGGT GTGAGAGCAG TCGGTCTCAC 
ACCACCATTG CCAGATATGC TCAATACCAG GCCTCATCTT TCCAGGAATC ATTGCGGGAG 
GAAAATGAAA AGAAAGGACA GAAAGACCAC TCGGATACGG AGTCTGCTCC ATCAGAAAAT 
GTGGTGCGGA GAAGAAGAGG TCCCTTTAAG CACATCAAAT TTGGAACCAA CATTGATTTG 
TCAGATGAGA AAAAGTGGAA GCTGCAGCTG GCTGAGTTGA GTAAACTGCC TGCCTTCGCA 
CGGGTGGTGT CTGCAGGGAA TCTGCTCAGT CATGTGGGCC ACACAATTCT CGGCATGAAC 
ACAGTCCAGC TCTATATGAA GGTCCCGGGC AGCAGGACAC CAGGTCATCA AGAAAACAAC 
AACTTCTGCT CTGTGAACAT AAACATTGGC CCTGGAGATT GTGAATGGTT TGCCGTTCCT 
GAGCCGTACT GGGGTGTCAT GAATGATTTC TGTGAAAAGA ATAATATTAA TTATCTAATG 
GGATCTTGGT GGCCAAATCT TGAGGACTTG TATGAGGCAA ATGTTCCAGT GTATCGCTTT 
ATCCAGCGAC CAGGTGATCT GGTCTGGCTC AATACAGGGA CTGTGCATTG GGTTCAGGCT 
ATTGGCTGGT GCAACAACAT TGCCTGGAAC GTAGGACCAC TCACTGTGCA TCAGTACAAG 
CTAGCGGTGG AGCGCTATGA GTGGAACAAA CTCCAGAGTG TCAAATCCAT CGTTCCCATG 
ATTCACCTCT CCTGGAATAT GGCCAGAAAC ATCAAAGTGT CAGACCACAA GCTCTTTGAG 
ATGATCAAGT ACTGTTTATT GAGGACACTA AAGCAGTGTC AGATGCTGAG GGAGGCTCTC 
ATAGCTGCTG GAAAAGAGCT TGTTTGGCAT GGAAGGACCA AGGACGAACC TGCTCACTAC 
TGTAGCATCT GTGAGGTGGA GGTCTTTGAT CTGCTGTTTG TCACCAGTGA AAGTAACTCA 
AGAAAGACGT ATGTGGTTCA CTGCCAGGGC TGCGCTCGCA GATGTAGCCC TAACCTGGAC 
AACTTTGTGG TTTTAGAACA GTACAAAATA GACGACCTCA TGCAAGTGTA CGACCAGTTC 
ACATTAGCTC CACCTCTGCC CTCCTCCTCC TCATCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm6a ( XM_005167795.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167852.1 Danio rerio lysine (K)-specific demethylase 6A (kdm6a), transcript variant X2, mRNA.

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CloneID	ODa15358
Clone ID Related Accession (Same CDS sequence)	XM_005167795.3 , XM_005167795.4
Accession Version	XM_005167795.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	lysine-specific demethylase 6A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005167795.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCGT GCGGAGTGTC GGTGGCCGCT GTAGCTCGCG CTGCTGCTGC TGCTGCTGCT 
GGTGGTGATG AGGAAAAGAA AATGGCGGCG GGAAAAGCGA GAGAAACCGA GGAGGACTTT 
CCGAAGCTTA CGCCGCAAGA AAGGGAGCGC TTAGCCAGCA TAGACAGCAC ACTGTTTGGA 
TTTCAGAGGC TTCATGAAGA TGGCGCCAGA ACGAAGGCCT TGCTGTTAAA GGCTGTTCGC 
TGCTATGACG TCTATATTTT AAAAGCTGAA GGGAAAGTGG AGCCAGAGGT GTTTTGCCAG 
TTAGGTCACT TCAACCTTTT ACTGGAAGAT TATCCTAAAG CATTATCTGC ATACCAGAGA 
TACTACAGTT TACAGTCAGA CTACTGGAAG AATGCTGCCT TTTTATATGG CCTTGGTTTG 
GTTTACTTCC ATTACAATGC TTTTCGATGG GCGATCAAAG CATTTCAGGA GGTGCTTTAC 
ATTGACCCCT GCTTCTCAAG AGCCAAGGAG ATCCATCTGC GACTCGGTCT GATGTTTAAA 
GTCAACACGG ACTATGAGTC AAGCCTCAAG CATTTTCAGC TGGCATTGAT TGACTCCACA 
CCCTGCACTT TGTCCAAAGC TGAAATTCAG TTCCACATTG CTCATGTATA TGAAATTCAG 
AAGAAATACC GCATCGCAAA GGAAGCTTAC GAGAGTCTGT TACAAACAGA AAATCTCCCT 
GCACAGGTGA AGGCAACTAC GCTCCAACAA TTAGGCTGGA TGCATCACAC GGTCGAACAG 
TTGGGAGATA AAGCAAACAA GAACAGCTAT GCTATTCAGT GTCTGCAGAA ATCCCTAGAA 
GCAGACCCCA ATTCTGGACA GTCTTGGTAC TTCCTGGGCA GGTGCTACTC AAGTATTGGG 
AAGGTTCAAG ATGCCTTCAT ATCTTACCGA CAGTCTATTG ATAAGTCTGA GGCCAGTGCA 
GATACATGGT GCTCAATCGG TGTGTTGTAT CAGCAGCAGA ACCAGCCGAT GGACGCACTG 
CAGGCATATA TCTGTGCAGT ACAGCTGGAC CACAGCCATG CGGCTGCCTG GATGGACCTG 
GGCACGCTCT ACGAGTCCTG TAACCAGCCA CAGGATGCCA TCAAGTGCTA CATCAATGCC 
ACGTGCAGCA AGTCCTGCAG TAACATCCCA GCTCTAACTG CTCGCATCAA ATGCCTCCAG 
GCTCAGTTGT GTAATCTTCA GCAAGGTAGT TTACCAAGTA AAAGTAAAAT GCTCCCTAGT 
ATTGAGGAGG CGTGGAGCCT ACCAATCCCA GCAGAGCTCA CCTCCAGACA GGGAGCGCTC 
AACACGACCC AACAGCAAAC CACCTGCAAA GCTCATCAGA GCAATGAAGC CCAGTCTCAA 
ATCCCAAGCC AGTCTCTTCC TCCTCATGTG CTCTCTGGCC AGACAGAAGA CCAGTCCAGC 
CCGGCCAAGA AAAAGAGGAC CACTAGTCCA GCCAGGAATT CATCAGATTC CTGGACAAAT 
GATCCAGGTC ATCATCAGAT CTCCAACTGG AGTTTAACCC CACAGAAACT TCAGCTCCTG 
GAACAGTTAC GGAACAACAG AGCCAATCTG AAGCCAGTGC AGCTGCAGAT GCTGGAACAG 
TTGGAAAAGC AACTGAGTCT AATGCAACAG AATCAACAGA CAAGGCTGAA TGCCATGGGT 
CAGATACGAC CCAGTGTGTC CAACGGTCCT GTAGCCGACT CTTCACTGCC TACAAACTCT 
AATTCCACAC CTTCCCCTCA CCATCCACAC ATCGCTCTGT CTCGCACACC CTCTGCCCTC 
ACACGATGCG CTGCTCAGCC AATAGCCAAC GGACCTGTCC CTGCGAGCCC CGTCCCATGC 
AGCACCGCTG GTACCCTGGG TAACACAGAT GCCGTCTCTG TGGGCAATAA TCATCTCCCA 
GGATCGGGGA GCAACGGAAA TGTGCCTTAC TTGCGGCAAA ACGCACTACC TCATAACTGC 
ACAACCCCCA CCAGCAGCAG CATGGATGAT GAACAGTGGA AAAGCCAACA CATCAACTCC 
ACTCAGGGGC TTCAGAAAAG TCCAGGTTCA TATTCAGCAG GTCCTAACGG CGAGCAGCCT 
TTCTCTTCCA CTGGGACGTC CCAGCCTTCC CCGGCAGCCG GCACTGGCGG TCCAAATCAG 
GTCGGACATT CGGCCACAGC CAGCGACACG CAAGCGCAGG AGGCCAGTCA CAATCACCTC 
CCCTCCCCGA GCTCCCAGAG CTCTGCTACC TCAGGTGGAC AGCAAGGCAC GGTGAACACC 
AAAGAGAGCA AGCCTTCAGG AAACGGGCAT TCCGTGGGGA CGCCTAACAG CACTGCCACT 
GCAGAGGGAC TGCCTAATCA TGTCCATCAG GGCCAGGCAG ACACTGCTGG GGCGAAACCC 
CGTTCACCAG GTGTACTTAG TTCAGACAAT CCACAGCTCT CGGCCTTGTT GATTGGAAAA 
GCCAATGATA GTAATAATAG TAATGGAGGA GGGTCGGCGG CTGCTGGCAC AAAGGTTAAC 
AATGTTCACC CGAGTCTGCA CAAGCCGCAG GAAAACTCGG TGGCTTCTTC TCCATGTTCT 
GCCATGTCTG CAGCTTCGCC CTCACCCAAA ACTGCAGACC ACCACAGTAC TCAGAACAGT 
GTTAACAGCC TTAACAGCCC CACGCTCAAC GGCAAAGGTC TGGAGGACTC TCAGAGCCCC 
TTGAAGGTGG CTTCTCCACT GGTTTGTCAT AAACCAACGC CTCCGTCGTT CACTCTGTGG 
TCCTCTGTGT CCATCTACCC CAGCTCCAGT GATGTGCTCA AAGCATGCAG AAACCTGGGG 
AAGAATGGTG TGTCCAATAA CAGCATCTTA CTAGACAAGT GTCCCCCTCC ACGGTTACCA 
CCTCCACCCA TCCCTCCTCT GCCAAAGGAC AAACTCAATC CTCCAACCCC TAGTATTTAT 
TTGGAGAACA AGAGGGATGC TTTCTTTCCA CCGCTGCACC AGTTCTGCAC AAACACAGCC 
AACCCTGTAA CAGTGATCCG TGGCCTGGCT GGAGCACTCA AGCTAGACCT TGGATTATTC 
TCCACCAAGA CTCTGGTTGA GGCTAACCCA GAGCACCTTG TGGAGGTGAG GACTCAGTTG 
TCTCAGCCCA CCGATGAGAA CTGGGATGTG ACAGGGAGCA GGAAGATGTG GCGGTGTGAG 
AGCAGTCGGT CTCACACCAC CATTGCCAGA TATGCTCAAT ACCAGGCCTC ATCTTTCCAG 
GAATCATTGC GGGAGGAAAA TGAAAAGAAA GGACAGAAAG ACCACTCGGA TACGGAGTCT 
GCTCCATCAG AAAATGTGGT GCGGAGAAGA AGAGGTCCCT TTAAGCACAT CAAATTTGGA 
ACCAACATTG ATTTGTCAGA TGAGAAAAAG TGGAAGCTGC AGCTGGCTGA GTTGAGTAAA 
CTGCCTGCCT TCGCACGGGT GGTGTCTGCA GGGAATCTGC TCAGTCATGT GGGCCACACA 
ATTCTCGGCA TGAACACAGT CCAGCTCTAT ATGAAGGTCC CGGGCAGCAG GACACCAGGT 
CATCAAGAAA ACAACAACTT CTGCTCTGTG AACATAAACA TTGGCCCTGG AGATTGTGAA 
TGGTTTGCCG TTCCTGAGCC GTACTGGGGT GTCATGAATG ATTTCTGTGA AAAGAATAAT 
ATTAATTATC TAATGGGATC TTGGTGGCCA AATCTTGAGG ACTTGTATGA GGCAAATGTT 
CCAGTGTATC GCTTTATCCA GCGACCAGGT GATCTGGTCT GGCTCAATAC AGGGACTGTG 
CATTGGGTTC AGGCTATTGG CTGGTGCAAC AACATTGCCT GGAACGTAGG ACCACTCACT 
GTGCATCAGT ACAAGCTAGC GGTGGAGCGC TATGAGTGGA ACAAACTCCA GAGTGTCAAA 
TCCATCGTTC CCATGATTCA CCTCTCCTGG AATATGGCCA GAAACATCAA AGTGTCAGAC 
CACAAGCTCT TTGAGATGAT CAAGTACTGT TTATTGAGGA CACTAAAGCA GTGTCAGATG 
CTGAGGGAGG CTCTCATAGC TGCTGGAAAA GAGCTTGTTT GGCATGGAAG GACCAAGGAC 
GAACCTGCTC ACTACTGTAG CATCTGTGAG GTGGAGGTCT TTGATCTGCT GTTTGTCACC 
AGTGAAAGTA ACTCAAGAAA GACGTATGTG GTTCACTGCC AGGGCTGCGC TCGCAGATGT 
AGCCCTAACC TGGACAACTT TGTGGTTTTA GAACAGTACA AAATAGACGA CCTCATGCAA 
GTGTACGACC AGTTCACATT AGCTCCACCT CTGCCCTCCT CCTCCTCATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167852.1
CDS	623..4936
Translation	MKSCGVSVAAVARAAAAAAAGGDEEKKMAAGKARETEEDFPKLTPQERERLASIDSTLFGFQRLHEDGARTKALLLKAVRCYDVYILKAEGKVEPEVFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFRWAIKAFQEVLYIDPCFSRAKEIHLRLGLMFKVNTDYESSLKHFQLALIDSTPCTLSKAEIQFHIAHVYEIQKKYRIAKEAYESLLQTENLPAQVKATTLQQLGWMHHTVEQLGDKANKNSYAIQCLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHSHAAAWMDLGTLYESCNQPQDAIKCYINATCSKSCSNIPALTARIKCLQAQLCNLQQGSLPSKSKMLPSIEEAWSLPIPAELTSRQGALNTTQQQTTCKAHQSNEAQSQIPSQSLPPHVLSGQTEDQSSPAKKKRTTSPARNSSDSWTNDPGHHQISNWSLTPQKLQLLEQLRNNRANLKPVQLQMLEQLEKQLSLMQQNQQTRLNAMGQIRPSVSNGPVADSSLPTNSNSTPSPHHPHIALSRTPSALTRCAAQPIANGPVPASPVPCSTAGTLGNTDAVSVGNNHLPGSGSNGNVPYLRQNALPHNCTTPTSSSMDDEQWKSQHINSTQGLQKSPGSYSAGPNGEQPFSSTGTSQPSPAAGTGGPNQVGHSATASDTQAQEASHNHLPSPSSQSSATSGGQQGTVNTKESKPSGNGHSVGTPNSTATAEGLPNHVHQGQADTAGAKPRSPGVLSSDNPQLSALLIGKANDSNNSNGGGSAAAGTKVNNVHPSLHKPQENSVASSPCSAMSAASPSPKTADHHSTQNSVNSLNSPTLNGKGLEDSQSPLKVASPLVCHKPTPPSFTLWSSVSIYPSSSDVLKACRNLGKNGVSNNSILLDKCPPPRLPPPPIPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNTANPVTVIRGLAGALKLDLGLFSTKTLVEANPEHLVEVRTQLSQPTDENWDVTGSRKMWRCESSRSHTTIARYAQYQASSFQESLREENEKKGQKDHSDTESAPSENVVRRRRGPFKHIKFGTNIDLSDEKKWKLQLAELSKLPAFARVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVPEPYWGVMNDFCEKNNINYLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWLNTGTVHWVQAIGWCNNIAWNVGPLTVHQYKLAVERYEWNKLQSVKSIVPMIHLSWNMARNIKVSDHKLFEMIKYCLLRTLKQCQMLREALIAAGKELVWHGRTKDEPAHYCSICEVEVFDLLFVTSESNSRKTYVVHCQGCARRCSPNLDNFVVLEQYKIDDLMQVYDQFTLAPPLPSSSSS

Target ORF information:

RefSeq Version	XM_005167795.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 6A (kdm6a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167795.3

ORF Insert Sequence:

ATGAAATCGT GCGGAGTGTC GGTGGCCGCT GTAGCTCGCG CTGCTGCTGC TGCTGCTGCT 
GGTGGTGATG AGGAAAAGAA AATGGCGGCG GGAAAAGCGA GAGAAACCGA GGAGGACTTT 
CCGAAGCTTA CGCCGCAAGA AAGGGAGCGC TTAGCCAGCA TAGACAGCAC ACTGTTTGGA 
TTTCAGAGGC TTCATGAAGA TGGCGCCAGA ACGAAGGCCT TGCTGTTAAA GGCTGTTCGC 
TGCTATGACG TCTATATTTT AAAAGCTGAA GGGAAAGTGG AGCCAGAGGT GTTTTGCCAG 
TTAGGTCACT TCAACCTTTT ACTGGAAGAT TATCCTAAAG CATTATCTGC ATACCAGAGA 
TACTACAGTT TACAGTCAGA CTACTGGAAG AATGCTGCCT TTTTATATGG CCTTGGTTTG 
GTTTACTTCC ATTACAATGC TTTTCGATGG GCGATCAAAG CATTTCAGGA GGTGCTTTAC 
ATTGACCCCT GCTTCTCAAG AGCCAAGGAG ATCCATCTGC GACTCGGTCT GATGTTTAAA 
GTCAACACGG ACTATGAGTC AAGCCTCAAG CATTTTCAGC TGGCATTGAT TGACTCCACA 
CCCTGCACTT TGTCCAAAGC TGAAATTCAG TTCCACATTG CTCATGTATA TGAAATTCAG 
AAGAAATACC GCATCGCAAA GGAAGCTTAC GAGAGTCTGT TACAAACAGA AAATCTCCCT 
GCACAGGTGA AGGCAACTAC GCTCCAACAA TTAGGCTGGA TGCATCACAC GGTCGAACAG 
TTGGGAGATA AAGCAAACAA GAACAGCTAT GCTATTCAGT GTCTGCAGAA ATCCCTAGAA 
GCAGACCCCA ATTCTGGACA GTCTTGGTAC TTCCTGGGCA GGTGCTACTC AAGTATTGGG 
AAGGTTCAAG ATGCCTTCAT ATCTTACCGA CAGTCTATTG ATAAGTCTGA GGCCAGTGCA 
GATACATGGT GCTCAATCGG TGTGTTGTAT CAGCAGCAGA ACCAGCCGAT GGACGCACTG 
CAGGCATATA TCTGTGCAGT ACAGCTGGAC CACAGCCATG CGGCTGCCTG GATGGACCTG 
GGCACGCTCT ACGAGTCCTG TAACCAGCCA CAGGATGCCA TCAAGTGCTA CATCAATGCC 
ACGTGCAGCA AGTCCTGCAG TAACATCCCA GCTCTAACTG CTCGCATCAA ATGCCTCCAG 
GCTCAGTTGT GTAATCTTCA GCAAGGTAGT TTACCAAGTA AAAGTAAAAT GCTCCCTAGT 
ATTGAGGAGG CGTGGAGCCT ACCAATCCCA GCAGAGCTCA CCTCCAGACA GGGAGCGCTC 
AACACGACCC AACAGCAAAC CACCTGCAAA GCTCATCAGA GCAATGAAGC CCAGTCTCAA 
ATCCCAAGCC AGTCTCTTCC TCCTCATGTG CTCTCTGGCC AGACAGAAGA CCAGTCCAGC 
CCGGCCAAGA AAAAGAGGAC CACTAGTCCA GCCAGGAATT CATCAGATTC CTGGACAAAT 
GATCCAGGTC ATCATCAGAT CTCCAACTGG AGTTTAACCC CACAGAAACT TCAGCTCCTG 
GAACAGTTAC GGAACAACAG AGCCAATCTG AAGCCAGTGC AGCTGCAGAT GCTGGAACAG 
TTGGAAAAGC AACTGAGTCT AATGCAACAG AATCAACAGA CAAGGCTGAA TGCCATGGGT 
CAGATACGAC CCAGTGTGTC CAACGGTCCT GTAGCCGACT CTTCACTGCC TACAAACTCT 
AATTCCACAC CTTCCCCTCA CCATCCACAC ATCGCTCTGT CTCGCACACC CTCTGCCCTC 
ACACGATGCG CTGCTCAGCC AATAGCCAAC GGACCTGTCC CTGCGAGCCC CGTCCCATGC 
AGCACCGCTG GTACCCTGGG TAACACAGAT GCCGTCTCTG TGGGCAATAA TCATCTCCCA 
GGATCGGGGA GCAACGGAAA TGTGCCTTAC TTGCGGCAAA ACGCACTACC TCATAACTGC 
ACAACCCCCA CCAGCAGCAG CATGGATGAT GAACAGTGGA AAAGCCAACA CATCAACTCC 
ACTCAGGGGC TTCAGAAAAG TCCAGGTTCA TATTCAGCAG GTCCTAACGG CGAGCAGCCT 
TTCTCTTCCA CTGGGACGTC CCAGCCTTCC CCGGCAGCCG GCACTGGCGG TCCAAATCAG 
GTCGGACATT CGGCCACAGC CAGCGACACG CAAGCGCAGG AGGCCAGTCA CAATCACCTC 
CCCTCCCCGA GCTCCCAGAG CTCTGCTACC TCAGGTGGAC AGCAAGGCAC GGTGAACACC 
AAAGAGAGCA AGCCTTCAGG AAACGGGCAT TCCGTGGGGA CGCCTAACAG CACTGCCACT 
GCAGAGGGAC TGCCTAATCA TGTCCATCAG GGCCAGGCAG ACACTGCTGG GGCGAAACCC 
CGTTCACCAG GTGTACTTAG TTCAGACAAT CCACAGCTCT CGGCCTTGTT GATTGGAAAA 
GCCAATGATA GTAATAATAG TAATGGAGGA GGGTCGGCGG CTGCTGGCAC AAAGGTTAAC 
AATGTTCACC CGAGTCTGCA CAAGCCGCAG GAAAACTCGG TGGCTTCTTC TCCATGTTCT 
GCCATGTCTG CAGCTTCGCC CTCACCCAAA ACTGCAGACC ACCACAGTAC TCAGAACAGT 
GTTAACAGCC TTAACAGCCC CACGCTCAAC GGCAAAGGTC TGGAGGACTC TCAGAGCCCC 
TTGAAGGTGG CTTCTCCACT GGTTTGTCAT AAACCAACGC CTCCGTCGTT CACTCTGTGG 
TCCTCTGTGT CCATCTACCC CAGCTCCAGT GATGTGCTCA AAGCATGCAG AAACCTGGGG 
AAGAATGGTG TGTCCAATAA CAGCATCTTA CTAGACAAGT GTCCCCCTCC ACGGTTACCA 
CCTCCACCCA TCCCTCCTCT GCCAAAGGAC AAACTCAATC CTCCAACCCC TAGTATTTAT 
TTGGAGAACA AGAGGGATGC TTTCTTTCCA CCGCTGCACC AGTTCTGCAC AAACACAGCC 
AACCCTGTAA CAGTGATCCG TGGCCTGGCT GGAGCACTCA AGCTAGACCT TGGATTATTC 
TCCACCAAGA CTCTGGTTGA GGCTAACCCA GAGCACCTTG TGGAGGTGAG GACTCAGTTG 
TCTCAGCCCA CCGATGAGAA CTGGGATGTG ACAGGGAGCA GGAAGATGTG GCGGTGTGAG 
AGCAGTCGGT CTCACACCAC CATTGCCAGA TATGCTCAAT ACCAGGCCTC ATCTTTCCAG 
GAATCATTGC GGGAGGAAAA TGAAAAGAAA GGACAGAAAG ACCACTCGGA TACGGAGTCT 
GCTCCATCAG AAAATGTGGT GCGGAGAAGA AGAGGTCCCT TTAAGCACAT CAAATTTGGA 
ACCAACATTG ATTTGTCAGA TGAGAAAAAG TGGAAGCTGC AGCTGGCTGA GTTGAGTAAA 
CTGCCTGCCT TCGCACGGGT GGTGTCTGCA GGGAATCTGC TCAGTCATGT GGGCCACACA 
ATTCTCGGCA TGAACACAGT CCAGCTCTAT ATGAAGGTCC CGGGCAGCAG GACACCAGGT 
CATCAAGAAA ACAACAACTT CTGCTCTGTG AACATAAACA TTGGCCCTGG AGATTGTGAA 
TGGTTTGCCG TTCCTGAGCC GTACTGGGGT GTCATGAATG ATTTCTGTGA AAAGAATAAT 
ATTAATTATC TAATGGGATC TTGGTGGCCA AATCTTGAGG ACTTGTATGA GGCAAATGTT 
CCAGTGTATC GCTTTATCCA GCGACCAGGT GATCTGGTCT GGCTCAATAC AGGGACTGTG 
CATTGGGTTC AGGCTATTGG CTGGTGCAAC AACATTGCCT GGAACGTAGG ACCACTCACT 
GTGCATCAGT ACAAGCTAGC GGTGGAGCGC TATGAGTGGA ACAAACTCCA GAGTGTCAAA 
TCCATCGTTC CCATGATTCA CCTCTCCTGG AATATGGCCA GAAACATCAA AGTGTCAGAC 
CACAAGCTCT TTGAGATGAT CAAGTACTGT TTATTGAGGA CACTAAAGCA GTGTCAGATG 
CTGAGGGAGG CTCTCATAGC TGCTGGAAAA GAGCTTGTTT GGCATGGAAG GACCAAGGAC 
GAACCTGCTC ACTACTGTAG CATCTGTGAG GTGGAGGTCT TTGATCTGCT GTTTGTCACC 
AGTGAAAGTA ACTCAAGAAA GACGTATGTG GTTCACTGCC AGGGCTGCGC TCGCAGATGT 
AGCCCTAACC TGGACAACTT TGTGGTTTTA GAACAGTACA AAATAGACGA CCTCATGCAA 
GTGTACGACC AGTTCACATT AGCTCCACCT CTGCCCTCCT CCTCCTCATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kdm6a ( XM_005167795.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005167852.1 Danio rerio lysine (K)-specific demethylase 6A (kdm6a), transcript variant X2, mRNA.

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CloneID	ODa15358
Clone ID Related Accession (Same CDS sequence)	XM_005167795.3 , XM_005167795.4
Accession Version	XM_005167795.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4314bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	lysine-specific demethylase 6A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005167795.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAATCGT GCGGAGTGTC GGTGGCCGCT GTAGCTCGCG CTGCTGCTGC TGCTGCTGCT 
GGTGGTGATG AGGAAAAGAA AATGGCGGCG GGAAAAGCGA GAGAAACCGA GGAGGACTTT 
CCGAAGCTTA CGCCGCAAGA AAGGGAGCGC TTAGCCAGCA TAGACAGCAC ACTGTTTGGA 
TTTCAGAGGC TTCATGAAGA TGGCGCCAGA ACGAAGGCCT TGCTGTTAAA GGCTGTTCGC 
TGCTATGACG TCTATATTTT AAAAGCTGAA GGGAAAGTGG AGCCAGAGGT GTTTTGCCAG 
TTAGGTCACT TCAACCTTTT ACTGGAAGAT TATCCTAAAG CATTATCTGC ATACCAGAGA 
TACTACAGTT TACAGTCAGA CTACTGGAAG AATGCTGCCT TTTTATATGG CCTTGGTTTG 
GTTTACTTCC ATTACAATGC TTTTCGATGG GCGATCAAAG CATTTCAGGA GGTGCTTTAC 
ATTGACCCCT GCTTCTCAAG AGCCAAGGAG ATCCATCTGC GACTCGGTCT GATGTTTAAA 
GTCAACACGG ACTATGAGTC AAGCCTCAAG CATTTTCAGC TGGCATTGAT TGACTCCACA 
CCCTGCACTT TGTCCAAAGC TGAAATTCAG TTCCACATTG CTCATGTATA TGAAATTCAG 
AAGAAATACC GCATCGCAAA GGAAGCTTAC GAGAGTCTGT TACAAACAGA AAATCTCCCT 
GCACAGGTGA AGGCAACTAC GCTCCAACAA TTAGGCTGGA TGCATCACAC GGTCGAACAG 
TTGGGAGATA AAGCAAACAA GAACAGCTAT GCTATTCAGT GTCTGCAGAA ATCCCTAGAA 
GCAGACCCCA ATTCTGGACA GTCTTGGTAC TTCCTGGGCA GGTGCTACTC AAGTATTGGG 
AAGGTTCAAG ATGCCTTCAT ATCTTACCGA CAGTCTATTG ATAAGTCTGA GGCCAGTGCA 
GATACATGGT GCTCAATCGG TGTGTTGTAT CAGCAGCAGA ACCAGCCGAT GGACGCACTG 
CAGGCATATA TCTGTGCAGT ACAGCTGGAC CACAGCCATG CGGCTGCCTG GATGGACCTG 
GGCACGCTCT ACGAGTCCTG TAACCAGCCA CAGGATGCCA TCAAGTGCTA CATCAATGCC 
ACGTGCAGCA AGTCCTGCAG TAACATCCCA GCTCTAACTG CTCGCATCAA ATGCCTCCAG 
GCTCAGTTGT GTAATCTTCA GCAAGGTAGT TTACCAAGTA AAAGTAAAAT GCTCCCTAGT 
ATTGAGGAGG CGTGGAGCCT ACCAATCCCA GCAGAGCTCA CCTCCAGACA GGGAGCGCTC 
AACACGACCC AACAGCAAAC CACCTGCAAA GCTCATCAGA GCAATGAAGC CCAGTCTCAA 
ATCCCAAGCC AGTCTCTTCC TCCTCATGTG CTCTCTGGCC AGACAGAAGA CCAGTCCAGC 
CCGGCCAAGA AAAAGAGGAC CACTAGTCCA GCCAGGAATT CATCAGATTC CTGGACAAAT 
GATCCAGGTC ATCATCAGAT CTCCAACTGG AGTTTAACCC CACAGAAACT TCAGCTCCTG 
GAACAGTTAC GGAACAACAG AGCCAATCTG AAGCCAGTGC AGCTGCAGAT GCTGGAACAG 
TTGGAAAAGC AACTGAGTCT AATGCAACAG AATCAACAGA CAAGGCTGAA TGCCATGGGT 
CAGATACGAC CCAGTGTGTC CAACGGTCCT GTAGCCGACT CTTCACTGCC TACAAACTCT 
AATTCCACAC CTTCCCCTCA CCATCCACAC ATCGCTCTGT CTCGCACACC CTCTGCCCTC 
ACACGATGCG CTGCTCAGCC AATAGCCAAC GGACCTGTCC CTGCGAGCCC CGTCCCATGC 
AGCACCGCTG GTACCCTGGG TAACACAGAT GCCGTCTCTG TGGGCAATAA TCATCTCCCA 
GGATCGGGGA GCAACGGAAA TGTGCCTTAC TTGCGGCAAA ACGCACTACC TCATAACTGC 
ACAACCCCCA CCAGCAGCAG CATGGATGAT GAACAGTGGA AAAGCCAACA CATCAACTCC 
ACTCAGGGGC TTCAGAAAAG TCCAGGTTCA TATTCAGCAG GTCCTAACGG CGAGCAGCCT 
TTCTCTTCCA CTGGGACGTC CCAGCCTTCC CCGGCAGCCG GCACTGGCGG TCCAAATCAG 
GTCGGACATT CGGCCACAGC CAGCGACACG CAAGCGCAGG AGGCCAGTCA CAATCACCTC 
CCCTCCCCGA GCTCCCAGAG CTCTGCTACC TCAGGTGGAC AGCAAGGCAC GGTGAACACC 
AAAGAGAGCA AGCCTTCAGG AAACGGGCAT TCCGTGGGGA CGCCTAACAG CACTGCCACT 
GCAGAGGGAC TGCCTAATCA TGTCCATCAG GGCCAGGCAG ACACTGCTGG GGCGAAACCC 
CGTTCACCAG GTGTACTTAG TTCAGACAAT CCACAGCTCT CGGCCTTGTT GATTGGAAAA 
GCCAATGATA GTAATAATAG TAATGGAGGA GGGTCGGCGG CTGCTGGCAC AAAGGTTAAC 
AATGTTCACC CGAGTCTGCA CAAGCCGCAG GAAAACTCGG TGGCTTCTTC TCCATGTTCT 
GCCATGTCTG CAGCTTCGCC CTCACCCAAA ACTGCAGACC ACCACAGTAC TCAGAACAGT 
GTTAACAGCC TTAACAGCCC CACGCTCAAC GGCAAAGGTC TGGAGGACTC TCAGAGCCCC 
TTGAAGGTGG CTTCTCCACT GGTTTGTCAT AAACCAACGC CTCCGTCGTT CACTCTGTGG 
TCCTCTGTGT CCATCTACCC CAGCTCCAGT GATGTGCTCA AAGCATGCAG AAACCTGGGG 
AAGAATGGTG TGTCCAATAA CAGCATCTTA CTAGACAAGT GTCCCCCTCC ACGGTTACCA 
CCTCCACCCA TCCCTCCTCT GCCAAAGGAC AAACTCAATC CTCCAACCCC TAGTATTTAT 
TTGGAGAACA AGAGGGATGC TTTCTTTCCA CCGCTGCACC AGTTCTGCAC AAACACAGCC 
AACCCTGTAA CAGTGATCCG TGGCCTGGCT GGAGCACTCA AGCTAGACCT TGGATTATTC 
TCCACCAAGA CTCTGGTTGA GGCTAACCCA GAGCACCTTG TGGAGGTGAG GACTCAGTTG 
TCTCAGCCCA CCGATGAGAA CTGGGATGTG ACAGGGAGCA GGAAGATGTG GCGGTGTGAG 
AGCAGTCGGT CTCACACCAC CATTGCCAGA TATGCTCAAT ACCAGGCCTC ATCTTTCCAG 
GAATCATTGC GGGAGGAAAA TGAAAAGAAA GGACAGAAAG ACCACTCGGA TACGGAGTCT 
GCTCCATCAG AAAATGTGGT GCGGAGAAGA AGAGGTCCCT TTAAGCACAT CAAATTTGGA 
ACCAACATTG ATTTGTCAGA TGAGAAAAAG TGGAAGCTGC AGCTGGCTGA GTTGAGTAAA 
CTGCCTGCCT TCGCACGGGT GGTGTCTGCA GGGAATCTGC TCAGTCATGT GGGCCACACA 
ATTCTCGGCA TGAACACAGT CCAGCTCTAT ATGAAGGTCC CGGGCAGCAG GACACCAGGT 
CATCAAGAAA ACAACAACTT CTGCTCTGTG AACATAAACA TTGGCCCTGG AGATTGTGAA 
TGGTTTGCCG TTCCTGAGCC GTACTGGGGT GTCATGAATG ATTTCTGTGA AAAGAATAAT 
ATTAATTATC TAATGGGATC TTGGTGGCCA AATCTTGAGG ACTTGTATGA GGCAAATGTT 
CCAGTGTATC GCTTTATCCA GCGACCAGGT GATCTGGTCT GGCTCAATAC AGGGACTGTG 
CATTGGGTTC AGGCTATTGG CTGGTGCAAC AACATTGCCT GGAACGTAGG ACCACTCACT 
GTGCATCAGT ACAAGCTAGC GGTGGAGCGC TATGAGTGGA ACAAACTCCA GAGTGTCAAA 
TCCATCGTTC CCATGATTCA CCTCTCCTGG AATATGGCCA GAAACATCAA AGTGTCAGAC 
CACAAGCTCT TTGAGATGAT CAAGTACTGT TTATTGAGGA CACTAAAGCA GTGTCAGATG 
CTGAGGGAGG CTCTCATAGC TGCTGGAAAA GAGCTTGTTT GGCATGGAAG GACCAAGGAC 
GAACCTGCTC ACTACTGTAG CATCTGTGAG GTGGAGGTCT TTGATCTGCT GTTTGTCACC 
AGTGAAAGTA ACTCAAGAAA GACGTATGTG GTTCACTGCC AGGGCTGCGC TCGCAGATGT 
AGCCCTAACC TGGACAACTT TGTGGTTTTA GAACAGTACA AAATAGACGA CCTCATGCAA 
GTGTACGACC AGTTCACATT AGCTCCACCT CTGCCCTCCT CCTCCTCATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005167852.1
CDS	467..4780
Translation	MKSCGVSVAAVARAAAAAAAGGDEEKKMAAGKARETEEDFPKLTPQERERLASIDSTLFGFQRLHEDGARTKALLLKAVRCYDVYILKAEGKVEPEVFCQLGHFNLLLEDYPKALSAYQRYYSLQSDYWKNAAFLYGLGLVYFHYNAFRWAIKAFQEVLYIDPCFSRAKEIHLRLGLMFKVNTDYESSLKHFQLALIDSTPCTLSKAEIQFHIAHVYEIQKKYRIAKEAYESLLQTENLPAQVKATTLQQLGWMHHTVEQLGDKANKNSYAIQCLQKSLEADPNSGQSWYFLGRCYSSIGKVQDAFISYRQSIDKSEASADTWCSIGVLYQQQNQPMDALQAYICAVQLDHSHAAAWMDLGTLYESCNQPQDAIKCYINATCSKSCSNIPALTARIKCLQAQLCNLQQGSLPSKSKMLPSIEEAWSLPIPAELTSRQGALNTTQQQTTCKAHQSNEAQSQIPSQSLPPHVLSGQTEDQSSPAKKKRTTSPARNSSDSWTNDPGHHQISNWSLTPQKLQLLEQLRNNRANLKPVQLQMLEQLEKQLSLMQQNQQTRLNAMGQIRPSVSNGPVADSSLPTNSNSTPSPHHPHIALSRTPSALTRCAAQPIANGPVPASPVPCSTAGTLGNTDAVSVGNNHLPGSGSNGNVPYLRQNALPHNCTTPTSSSMDDEQWKSQHINSTQGLQKSPGSYSAGPNGEQPFSSTGTSQPSPAAGTGGPNQVGHSATASDTQAQEASHNHLPSPSSQSSATSGGQQGTVNTKESKPSGNGHSVGTPNSTATAEGLPNHVHQGQADTAGAKPRSPGVLSSDNPQLSALLIGKANDSNNSNGGGSAAAGTKVNNVHPSLHKPQENSVASSPCSAMSAASPSPKTADHHSTQNSVNSLNSPTLNGKGLEDSQSPLKVASPLVCHKPTPPSFTLWSSVSIYPSSSDVLKACRNLGKNGVSNNSILLDKCPPPRLPPPPIPPLPKDKLNPPTPSIYLENKRDAFFPPLHQFCTNTANPVTVIRGLAGALKLDLGLFSTKTLVEANPEHLVEVRTQLSQPTDENWDVTGSRKMWRCESSRSHTTIARYAQYQASSFQESLREENEKKGQKDHSDTESAPSENVVRRRRGPFKHIKFGTNIDLSDEKKWKLQLAELSKLPAFARVVSAGNLLSHVGHTILGMNTVQLYMKVPGSRTPGHQENNNFCSVNINIGPGDCEWFAVPEPYWGVMNDFCEKNNINYLMGSWWPNLEDLYEANVPVYRFIQRPGDLVWLNTGTVHWVQAIGWCNNIAWNVGPLTVHQYKLAVERYEWNKLQSVKSIVPMIHLSWNMARNIKVSDHKLFEMIKYCLLRTLKQCQMLREALIAAGKELVWHGRTKDEPAHYCSICEVEVFDLLFVTSESNSRKTYVVHCQGCARRCSPNLDNFVVLEQYKIDDLMQVYDQFTLAPPLPSSSSS

Target ORF information:

RefSeq Version	XM_005167795.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio lysine (K)-specific demethylase 6A (kdm6a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005167795.4

ORF Insert Sequence:

ATGAAATCGT GCGGAGTGTC GGTGGCCGCT GTAGCTCGCG CTGCTGCTGC TGCTGCTGCT 
GGTGGTGATG AGGAAAAGAA AATGGCGGCG GGAAAAGCGA GAGAAACCGA GGAGGACTTT 
CCGAAGCTTA CGCCGCAAGA AAGGGAGCGC TTAGCCAGCA TAGACAGCAC ACTGTTTGGA 
TTTCAGAGGC TTCATGAAGA TGGCGCCAGA ACGAAGGCCT TGCTGTTAAA GGCTGTTCGC 
TGCTATGACG TCTATATTTT AAAAGCTGAA GGGAAAGTGG AGCCAGAGGT GTTTTGCCAG 
TTAGGTCACT TCAACCTTTT ACTGGAAGAT TATCCTAAAG CATTATCTGC ATACCAGAGA 
TACTACAGTT TACAGTCAGA CTACTGGAAG AATGCTGCCT TTTTATATGG CCTTGGTTTG 
GTTTACTTCC ATTACAATGC TTTTCGATGG GCGATCAAAG CATTTCAGGA GGTGCTTTAC 
ATTGACCCCT GCTTCTCAAG AGCCAAGGAG ATCCATCTGC GACTCGGTCT GATGTTTAAA 
GTCAACACGG ACTATGAGTC AAGCCTCAAG CATTTTCAGC TGGCATTGAT TGACTCCACA 
CCCTGCACTT TGTCCAAAGC TGAAATTCAG TTCCACATTG CTCATGTATA TGAAATTCAG 
AAGAAATACC GCATCGCAAA GGAAGCTTAC GAGAGTCTGT TACAAACAGA AAATCTCCCT 
GCACAGGTGA AGGCAACTAC GCTCCAACAA TTAGGCTGGA TGCATCACAC GGTCGAACAG 
TTGGGAGATA AAGCAAACAA GAACAGCTAT GCTATTCAGT GTCTGCAGAA ATCCCTAGAA 
GCAGACCCCA ATTCTGGACA GTCTTGGTAC TTCCTGGGCA GGTGCTACTC AAGTATTGGG 
AAGGTTCAAG ATGCCTTCAT ATCTTACCGA CAGTCTATTG ATAAGTCTGA GGCCAGTGCA 
GATACATGGT GCTCAATCGG TGTGTTGTAT CAGCAGCAGA ACCAGCCGAT GGACGCACTG 
CAGGCATATA TCTGTGCAGT ACAGCTGGAC CACAGCCATG CGGCTGCCTG GATGGACCTG 
GGCACGCTCT ACGAGTCCTG TAACCAGCCA CAGGATGCCA TCAAGTGCTA CATCAATGCC 
ACGTGCAGCA AGTCCTGCAG TAACATCCCA GCTCTAACTG CTCGCATCAA ATGCCTCCAG 
GCTCAGTTGT GTAATCTTCA GCAAGGTAGT TTACCAAGTA AAAGTAAAAT GCTCCCTAGT 
ATTGAGGAGG CGTGGAGCCT ACCAATCCCA GCAGAGCTCA CCTCCAGACA GGGAGCGCTC 
AACACGACCC AACAGCAAAC CACCTGCAAA GCTCATCAGA GCAATGAAGC CCAGTCTCAA 
ATCCCAAGCC AGTCTCTTCC TCCTCATGTG CTCTCTGGCC AGACAGAAGA CCAGTCCAGC 
CCGGCCAAGA AAAAGAGGAC CACTAGTCCA GCCAGGAATT CATCAGATTC CTGGACAAAT 
GATCCAGGTC ATCATCAGAT CTCCAACTGG AGTTTAACCC CACAGAAACT TCAGCTCCTG 
GAACAGTTAC GGAACAACAG AGCCAATCTG AAGCCAGTGC AGCTGCAGAT GCTGGAACAG 
TTGGAAAAGC AACTGAGTCT AATGCAACAG AATCAACAGA CAAGGCTGAA TGCCATGGGT 
CAGATACGAC CCAGTGTGTC CAACGGTCCT GTAGCCGACT CTTCACTGCC TACAAACTCT 
AATTCCACAC CTTCCCCTCA CCATCCACAC ATCGCTCTGT CTCGCACACC CTCTGCCCTC 
ACACGATGCG CTGCTCAGCC AATAGCCAAC GGACCTGTCC CTGCGAGCCC CGTCCCATGC 
AGCACCGCTG GTACCCTGGG TAACACAGAT GCCGTCTCTG TGGGCAATAA TCATCTCCCA 
GGATCGGGGA GCAACGGAAA TGTGCCTTAC TTGCGGCAAA ACGCACTACC TCATAACTGC 
ACAACCCCCA CCAGCAGCAG CATGGATGAT GAACAGTGGA AAAGCCAACA CATCAACTCC 
ACTCAGGGGC TTCAGAAAAG TCCAGGTTCA TATTCAGCAG GTCCTAACGG CGAGCAGCCT 
TTCTCTTCCA CTGGGACGTC CCAGCCTTCC CCGGCAGCCG GCACTGGCGG TCCAAATCAG 
GTCGGACATT CGGCCACAGC CAGCGACACG CAAGCGCAGG AGGCCAGTCA CAATCACCTC 
CCCTCCCCGA GCTCCCAGAG CTCTGCTACC TCAGGTGGAC AGCAAGGCAC GGTGAACACC 
AAAGAGAGCA AGCCTTCAGG AAACGGGCAT TCCGTGGGGA CGCCTAACAG CACTGCCACT 
GCAGAGGGAC TGCCTAATCA TGTCCATCAG GGCCAGGCAG ACACTGCTGG GGCGAAACCC 
CGTTCACCAG GTGTACTTAG TTCAGACAAT CCACAGCTCT CGGCCTTGTT GATTGGAAAA 
GCCAATGATA GTAATAATAG TAATGGAGGA GGGTCGGCGG CTGCTGGCAC AAAGGTTAAC 
AATGTTCACC CGAGTCTGCA CAAGCCGCAG GAAAACTCGG TGGCTTCTTC TCCATGTTCT 
GCCATGTCTG CAGCTTCGCC CTCACCCAAA ACTGCAGACC ACCACAGTAC TCAGAACAGT 
GTTAACAGCC TTAACAGCCC CACGCTCAAC GGCAAAGGTC TGGAGGACTC TCAGAGCCCC 
TTGAAGGTGG CTTCTCCACT GGTTTGTCAT AAACCAACGC CTCCGTCGTT CACTCTGTGG 
TCCTCTGTGT CCATCTACCC CAGCTCCAGT GATGTGCTCA AAGCATGCAG AAACCTGGGG 
AAGAATGGTG TGTCCAATAA CAGCATCTTA CTAGACAAGT GTCCCCCTCC ACGGTTACCA 
CCTCCACCCA TCCCTCCTCT GCCAAAGGAC AAACTCAATC CTCCAACCCC TAGTATTTAT 
TTGGAGAACA AGAGGGATGC TTTCTTTCCA CCGCTGCACC AGTTCTGCAC AAACACAGCC 
AACCCTGTAA CAGTGATCCG TGGCCTGGCT GGAGCACTCA AGCTAGACCT TGGATTATTC 
TCCACCAAGA CTCTGGTTGA GGCTAACCCA GAGCACCTTG TGGAGGTGAG GACTCAGTTG 
TCTCAGCCCA CCGATGAGAA CTGGGATGTG ACAGGGAGCA GGAAGATGTG GCGGTGTGAG 
AGCAGTCGGT CTCACACCAC CATTGCCAGA TATGCTCAAT ACCAGGCCTC ATCTTTCCAG 
GAATCATTGC GGGAGGAAAA TGAAAAGAAA GGACAGAAAG ACCACTCGGA TACGGAGTCT 
GCTCCATCAG AAAATGTGGT GCGGAGAAGA AGAGGTCCCT TTAAGCACAT CAAATTTGGA 
ACCAACATTG ATTTGTCAGA TGAGAAAAAG TGGAAGCTGC AGCTGGCTGA GTTGAGTAAA 
CTGCCTGCCT TCGCACGGGT GGTGTCTGCA GGGAATCTGC TCAGTCATGT GGGCCACACA 
ATTCTCGGCA TGAACACAGT CCAGCTCTAT ATGAAGGTCC CGGGCAGCAG GACACCAGGT 
CATCAAGAAA ACAACAACTT CTGCTCTGTG AACATAAACA TTGGCCCTGG AGATTGTGAA 
TGGTTTGCCG TTCCTGAGCC GTACTGGGGT GTCATGAATG ATTTCTGTGA AAAGAATAAT 
ATTAATTATC TAATGGGATC TTGGTGGCCA AATCTTGAGG ACTTGTATGA GGCAAATGTT 
CCAGTGTATC GCTTTATCCA GCGACCAGGT GATCTGGTCT GGCTCAATAC AGGGACTGTG 
CATTGGGTTC AGGCTATTGG CTGGTGCAAC AACATTGCCT GGAACGTAGG ACCACTCACT 
GTGCATCAGT ACAAGCTAGC GGTGGAGCGC TATGAGTGGA ACAAACTCCA GAGTGTCAAA 
TCCATCGTTC CCATGATTCA CCTCTCCTGG AATATGGCCA GAAACATCAA AGTGTCAGAC 
CACAAGCTCT TTGAGATGAT CAAGTACTGT TTATTGAGGA CACTAAAGCA GTGTCAGATG 
CTGAGGGAGG CTCTCATAGC TGCTGGAAAA GAGCTTGTTT GGCATGGAAG GACCAAGGAC 
GAACCTGCTC ACTACTGTAG CATCTGTGAG GTGGAGGTCT TTGATCTGCT GTTTGTCACC 
AGTGAAAGTA ACTCAAGAAA GACGTATGTG GTTCACTGCC AGGGCTGCGC TCGCAGATGT 
AGCCCTAACC TGGACAACTT TGTGGTTTTA GAACAGTACA AAATAGACGA CCTCATGCAA 
GTGTACGACC AGTTCACATT AGCTCCACCT CTGCCCTCCT CCTCCTCATC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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kdm6a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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