git1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	git1
Entrez Gene ID	568935
Full Name	G protein-coupled receptor kinase interacting ArfGAP 1
General protein information	Preferred Names G protein-coupled receptor kinase interacting ArfGAP 1 Names ARF GTPase-activating protein GIT1 G protein-coupled receptor kinase-interactor 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_692294.7	XP_697386.4	ARF GTPase-activating protein GIT1 isoform X1
XM_009291756.1	XP_009290031.1	ARF GTPase-activating protein GIT1 isoform X3
XM_692294.8	XP_697386.4	ARF GTPase-activating protein GIT1 isoform X1
XM_009291756.2	XP_009290031.1	ARF GTPase-activating protein GIT1 isoform X3
XM_005157670.2	XP_005157727.1	ARF GTPase-activating protein GIT1 isoform X2
XM_005157670.3	XP_005157727.1	ARF GTPase-activating protein GIT1 isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC568935	XP_697386.4
Mus musculus (house mouse)	Git1	NP_001004144.1
Gallus gallus (chicken)	GIT1	NP_989627.1
Homo sapiens (human)	GIT1	NP_001078923.1
	GIT1	XP_001108023.2
Rattus norvegicus (Norway rat)	Git1	NP_114002.1
Pan troglodytes (chimpanzee)	GIT1	XP_511377.3
Canis lupus familiaris (dog)	GIT1	XP_005625057.1
Bos taurus (cattle)	GIT1	XP_002695750.3

Gene Ontology

The following git1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the git1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa20577	XM_692294.8 Latest version!	Danio rerio G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa20578	XM_005157670.3 Latest version!	Danio rerio G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa45617	XM_009291756.2 Latest version!	Danio rerio G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa20577	XM_692294.7	Danio rerio G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa45617	XM_009291756.1	Danio rerio G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa20578	XM_005157670.2	Danio rerio G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

git1 ( XM_692294.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_697386.4 Danio rerio G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa20577
Clone ID Related Accession (Same CDS sequence)	XM_692294.7 , XM_692294.8
Accession Version	XM_692294.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ARF GTPase-activating protein GIT1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_692294.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGGA AAGTCCAGAG AAGCGAGGTG TGCGCCGACT GCAGTGCACC AGACCCGGGC 
TGGAGCAGTA TAAACCGAGG AGTCCTGATC TGCGATGAGT GCTGTTCTGT ACACCGGAGC 
CTGGGCCGTC ACATTTCCAT CGTCAAACAC TTGAGACACA GCGGCTGGCC TCCTTCCTTA 
CTGCAGATGG TGCAGACCCT GGCCAGTAAC GGCGCTAACT CGATCTGGGA ACACTCTCTG 
CTGGATCCAG CACAAGTTCA GAGTGGCCGC AGGAAACCAA ACCCACAAGA TAAAGTCCAC 
CCCACAAAGT CAGAGTTCAT TCGCGCCAAG TACCAGATGT TAGCCTTTGT CCATAAGTTG 
CCCTGTCGTG ATGACGACGG AGTCACTACT AAGGACCTTA GCAAGCAACT GCACTCAAGT 
GTGCGGACAG GAAGTCTGGA GACATGTTTA CGGCTGCTGT CACTGGGGGC ACAAGCCAAC 
TTCTTCCACC CGGAAAAGGG CACAACCCCT CTCCATGTGG CTGCCAAAGC GGGACAGGTT 
CTCCAGGCTG AGCTGCTGGT TGTGTATGGA GCGGATCCGG GAGCTCCAGA TATCAATGGC 
CGAACACCCA TAGACTATGC ACGGCAAGCG GGTCACGTGG AGTTGGCCGA ACGCTTGGTG 
GAGTGTCAGT ATGAGCTCAC GGACAGACTG GCCTTCTACC TGTGTGGACG TCGCCCAGAT 
CATAAGAATG GTCATTATAT CATCCCTCAG ATGGCAGACA GAGCTCGCCC TAAGTGCCCG 
ACGCAGAGCC TGGACCTCTC TGAACTGGCC AAGGCAGCCA AGAAGAAGCT CCAAGCGTTA 
AATAATCGTT TGTTCGAGGA GCTGGCTATG GACGTGTACG ATGAAGTGGA CCGTAGAGAG 
AATGATGCAG TGTGGCTGAC AACTCAGAAC CACAGTACGC TGGTGACCGA ACGGAGCGCT 
GTCCCATTCT TACCCGTCAA TCCTGAATAC TCAGCGACAC GCAACCAGGG GCGGCAGAAA 
TTAGCACGCT TCAATGCGCG GGAATTCGCC ACCCTCATTA TCGACATCCT CAGCGACGCC 
AAGCGACGGC AACATGGAAA AGGCCTGACG AGCCCCACAG ACCCGCTGGA TCTGAGTCAG 
GTGGACGATG ACCAGCACGA TTATGACAGC GTAGCGTCTG ATGAAGACAC AGACAGCGAG 
CTGACCGCAC AGAATAATAA CAACACCCAA CGCAACAACC GGGCCAAGAG TATGGACTCG 
TCAGATCTGT CAGATGGACC AATCACACTA CAGGAGTATC TGGAGGTGAA GAAAGCACTG 
GCTTCCTCTG AGGCTAAAGT TCAGCAGCTC ATGAAGGTCA ACAACAACCT GAGTGAGGAG 
CTGCGGAGGC TGCAGAAAGA GATCTCGCGG ATGCAGACGG AGAACAGCGC GCTGCGGGGG 
GCCCAGGCTG GGTCGGGGGC CACCATGACT GGAGGTGGTG TTCCAGGACA CTGGCCTGAT 
AGAGTAAGGG GTATGGGTGG TGGAGGAGGG CTAAGTGGAG ATTCCAGCCT AATTGCACCC 
TCGTCCACCC CCCACCGACG GGACAGGCAG GCTTTCTCTA TGTATGAGCC TGTGGGCACC 
ACCTCAAAAG TCCTCAGCCC AGCCCTGGAC CCCCTGACTT GCCGCCTGCA GCCTCTCAGC 
CCTGTGCGAA AGGGCGCTCC AGCGGGTCCC ACCCCATACG GAGGAGCGCA CCTGTCAGCC 
TCCACTGACG GACGCTATAA TCATCCAAAA GCAGGAGAGA AGTACGGGAG TGGGACTGAT 
AGCGACTATG ACAACTCTCA AACATATGAC GTCTCCATTG GTGTGGGCCG CAGCAGTGAG 
GAGGACAGCA GGGGGGATGT AGACGACACC GAGGGCGATC CTGACCCCAC ACTGCCCTGC 
ACAGAGGACG TCATCCTTAA AACTGAACAG GTCACCAAAA ACATCCAGGA GCTCCTGAGA 
GCTGCTCAAG AGTTCAAGCA CGACAGTTTT GTGCCATGCT CAGAGAAAAT CCACTTAGCT 
GTGACAGAAA TGGCCTCGCT GTTTCCCAAG AGACCAGCGC TGGATGCAGT ACGTTCTTCC 
TTGAAGCTCC TGGCAGCAAG TGCGTCACGT TTGCAGGTGG AGTGTCGTAA AGCGGCGCCT 
TCGGACTCGT CGGCCAGTAC AGTGGACTAC CAGCTTCTTA CCCAGCAGGT CATCCAGTGC 
GCATACGACA TTGCCAAAGC CGCCAAGCAG CTGGTCACCA TCACCACCCG CGAAAAAAAA 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_697386.4
CDS	467..2752
Translation	MSRKVQRSEVCADCSAPDPGWSSINRGVLICDECCSVHRSLGRHISIVKHLRHSGWPPSLLQMVQTLASNGANSIWEHSLLDPAQVQSGRRKPNPQDKVHPTKSEFIRAKYQMLAFVHKLPCRDDDGVTTKDLSKQLHSSVRTGSLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQVLQAELLVVYGADPGAPDINGRTPIDYARQAGHVELAERLVECQYELTDRLAFYLCGRRPDHKNGHYIIPQMADRARPKCPTQSLDLSELAKAAKKKLQALNNRLFEELAMDVYDEVDRRENDAVWLTTQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSDAKRRQHGKGLTSPTDPLDLSQVDDDQHDYDSVASDEDTDSELTAQNNNNTQRNNRAKSMDSSDLSDGPITLQEYLEVKKALASSEAKVQQLMKVNNNLSEELRRLQKEISRMQTENSALRGAQAGSGATMTGGGVPGHWPDRVRGMGGGGGLSGDSSLIAPSSTPHRRDRQAFSMYEPVGTTSKVLSPALDPLTCRLQPLSPVRKGAPAGPTPYGGAHLSASTDGRYNHPKAGEKYGSGTDSDYDNSQTYDVSIGVGRSSEEDSRGDVDDTEGDPDPTLPCTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAVTEMASLFPKRPALDAVRSSLKLLAASASRLQVECRKAAPSDSSASTVDYQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_692294.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_692294.7

ORF Insert Sequence:

ATGTCCAGGA AAGTCCAGAG AAGCGAGGTG TGCGCCGACT GCAGTGCACC AGACCCGGGC 
TGGAGCAGTA TAAACCGAGG AGTCCTGATC TGCGATGAGT GCTGTTCTGT ACACCGGAGC 
CTGGGCCGTC ACATTTCCAT CGTCAAACAC TTGAGACACA GCGGCTGGCC TCCTTCCTTA 
CTGCAGATGG TGCAGACCCT GGCCAGTAAC GGCGCTAACT CGATCTGGGA ACACTCTCTG 
CTGGATCCAG CACAAGTTCA GAGTGGCCGC AGGAAACCAA ACCCACAAGA TAAAGTCCAC 
CCCACAAAGT CAGAGTTCAT TCGCGCCAAG TACCAGATGT TAGCCTTTGT CCATAAGTTG 
CCCTGTCGTG ATGACGACGG AGTCACTACT AAGGACCTTA GCAAGCAACT GCACTCAAGT 
GTGCGGACAG GAAGTCTGGA GACATGTTTA CGGCTGCTGT CACTGGGGGC ACAAGCCAAC 
TTCTTCCACC CGGAAAAGGG CACAACCCCT CTCCATGTGG CTGCCAAAGC GGGACAGGTT 
CTCCAGGCTG AGCTGCTGGT TGTGTATGGA GCGGATCCGG GAGCTCCAGA TATCAATGGC 
CGAACACCCA TAGACTATGC ACGGCAAGCG GGTCACGTGG AGTTGGCCGA ACGCTTGGTG 
GAGTGTCAGT ATGAGCTCAC GGACAGACTG GCCTTCTACC TGTGTGGACG TCGCCCAGAT 
CATAAGAATG GTCATTATAT CATCCCTCAG ATGGCAGACA GAGCTCGCCC TAAGTGCCCG 
ACGCAGAGCC TGGACCTCTC TGAACTGGCC AAGGCAGCCA AGAAGAAGCT CCAAGCGTTA 
AATAATCGTT TGTTCGAGGA GCTGGCTATG GACGTGTACG ATGAAGTGGA CCGTAGAGAG 
AATGATGCAG TGTGGCTGAC AACTCAGAAC CACAGTACGC TGGTGACCGA ACGGAGCGCT 
GTCCCATTCT TACCCGTCAA TCCTGAATAC TCAGCGACAC GCAACCAGGG GCGGCAGAAA 
TTAGCACGCT TCAATGCGCG GGAATTCGCC ACCCTCATTA TCGACATCCT CAGCGACGCC 
AAGCGACGGC AACATGGAAA AGGCCTGACG AGCCCCACAG ACCCGCTGGA TCTGAGTCAG 
GTGGACGATG ACCAGCACGA TTATGACAGC GTAGCGTCTG ATGAAGACAC AGACAGCGAG 
CTGACCGCAC AGAATAATAA CAACACCCAA CGCAACAACC GGGCCAAGAG TATGGACTCG 
TCAGATCTGT CAGATGGACC AATCACACTA CAGGAGTATC TGGAGGTGAA GAAAGCACTG 
GCTTCCTCTG AGGCTAAAGT TCAGCAGCTC ATGAAGGTCA ACAACAACCT GAGTGAGGAG 
CTGCGGAGGC TGCAGAAAGA GATCTCGCGG ATGCAGACGG AGAACAGCGC GCTGCGGGGG 
GCCCAGGCTG GGTCGGGGGC CACCATGACT GGAGGTGGTG TTCCAGGACA CTGGCCTGAT 
AGAGTAAGGG GTATGGGTGG TGGAGGAGGG CTAAGTGGAG ATTCCAGCCT AATTGCACCC 
TCGTCCACCC CCCACCGACG GGACAGGCAG GCTTTCTCTA TGTATGAGCC TGTGGGCACC 
ACCTCAAAAG TCCTCAGCCC AGCCCTGGAC CCCCTGACTT GCCGCCTGCA GCCTCTCAGC 
CCTGTGCGAA AGGGCGCTCC AGCGGGTCCC ACCCCATACG GAGGAGCGCA CCTGTCAGCC 
TCCACTGACG GACGCTATAA TCATCCAAAA GCAGGAGAGA AGTACGGGAG TGGGACTGAT 
AGCGACTATG ACAACTCTCA AACATATGAC GTCTCCATTG GTGTGGGCCG CAGCAGTGAG 
GAGGACAGCA GGGGGGATGT AGACGACACC GAGGGCGATC CTGACCCCAC ACTGCCCTGC 
ACAGAGGACG TCATCCTTAA AACTGAACAG GTCACCAAAA ACATCCAGGA GCTCCTGAGA 
GCTGCTCAAG AGTTCAAGCA CGACAGTTTT GTGCCATGCT CAGAGAAAAT CCACTTAGCT 
GTGACAGAAA TGGCCTCGCT GTTTCCCAAG AGACCAGCGC TGGATGCAGT ACGTTCTTCC 
TTGAAGCTCC TGGCAGCAAG TGCGTCACGT TTGCAGGTGG AGTGTCGTAA AGCGGCGCCT 
TCGGACTCGT CGGCCAGTAC AGTGGACTAC CAGCTTCTTA CCCAGCAGGT CATCCAGTGC 
GCATACGACA TTGCCAAAGC CGCCAAGCAG CTGGTCACCA TCACCACCCG CGAAAAAAAA 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

git1 ( XM_692294.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_697386.4 Danio rerio G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa20577
Clone ID Related Accession (Same CDS sequence)	XM_692294.7 , XM_692294.8
Accession Version	XM_692294.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ARF GTPase-activating protein GIT1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_692294.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGGA AAGTCCAGAG AAGCGAGGTG TGCGCCGACT GCAGTGCACC AGACCCGGGC 
TGGAGCAGTA TAAACCGAGG AGTCCTGATC TGCGATGAGT GCTGTTCTGT ACACCGGAGC 
CTGGGCCGTC ACATTTCCAT CGTCAAACAC TTGAGACACA GCGGCTGGCC TCCTTCCTTA 
CTGCAGATGG TGCAGACCCT GGCCAGTAAC GGCGCTAACT CGATCTGGGA ACACTCTCTG 
CTGGATCCAG CACAAGTTCA GAGTGGCCGC AGGAAACCAA ACCCACAAGA TAAAGTCCAC 
CCCACAAAGT CAGAGTTCAT TCGCGCCAAG TACCAGATGT TAGCCTTTGT CCATAAGTTG 
CCCTGTCGTG ATGACGACGG AGTCACTACT AAGGACCTTA GCAAGCAACT GCACTCAAGT 
GTGCGGACAG GAAGTCTGGA GACATGTTTA CGGCTGCTGT CACTGGGGGC ACAAGCCAAC 
TTCTTCCACC CGGAAAAGGG CACAACCCCT CTCCATGTGG CTGCCAAAGC GGGACAGGTT 
CTCCAGGCTG AGCTGCTGGT TGTGTATGGA GCGGATCCGG GAGCTCCAGA TATCAATGGC 
CGAACACCCA TAGACTATGC ACGGCAAGCG GGTCACGTGG AGTTGGCCGA ACGCTTGGTG 
GAGTGTCAGT ATGAGCTCAC GGACAGACTG GCCTTCTACC TGTGTGGACG TCGCCCAGAT 
CATAAGAATG GTCATTATAT CATCCCTCAG ATGGCAGACA GAGCTCGCCC TAAGTGCCCG 
ACGCAGAGCC TGGACCTCTC TGAACTGGCC AAGGCAGCCA AGAAGAAGCT CCAAGCGTTA 
AATAATCGTT TGTTCGAGGA GCTGGCTATG GACGTGTACG ATGAAGTGGA CCGTAGAGAG 
AATGATGCAG TGTGGCTGAC AACTCAGAAC CACAGTACGC TGGTGACCGA ACGGAGCGCT 
GTCCCATTCT TACCCGTCAA TCCTGAATAC TCAGCGACAC GCAACCAGGG GCGGCAGAAA 
TTAGCACGCT TCAATGCGCG GGAATTCGCC ACCCTCATTA TCGACATCCT CAGCGACGCC 
AAGCGACGGC AACATGGAAA AGGCCTGACG AGCCCCACAG ACCCGCTGGA TCTGAGTCAG 
GTGGACGATG ACCAGCACGA TTATGACAGC GTAGCGTCTG ATGAAGACAC AGACAGCGAG 
CTGACCGCAC AGAATAATAA CAACACCCAA CGCAACAACC GGGCCAAGAG TATGGACTCG 
TCAGATCTGT CAGATGGACC AATCACACTA CAGGAGTATC TGGAGGTGAA GAAAGCACTG 
GCTTCCTCTG AGGCTAAAGT TCAGCAGCTC ATGAAGGTCA ACAACAACCT GAGTGAGGAG 
CTGCGGAGGC TGCAGAAAGA GATCTCGCGG ATGCAGACGG AGAACAGCGC GCTGCGGGGG 
GCCCAGGCTG GGTCGGGGGC CACCATGACT GGAGGTGGTG TTCCAGGACA CTGGCCTGAT 
AGAGTAAGGG GTATGGGTGG TGGAGGAGGG CTAAGTGGAG ATTCCAGCCT AATTGCACCC 
TCGTCCACCC CCCACCGACG GGACAGGCAG GCTTTCTCTA TGTATGAGCC TGTGGGCACC 
ACCTCAAAAG TCCTCAGCCC AGCCCTGGAC CCCCTGACTT GCCGCCTGCA GCCTCTCAGC 
CCTGTGCGAA AGGGCGCTCC AGCGGGTCCC ACCCCATACG GAGGAGCGCA CCTGTCAGCC 
TCCACTGACG GACGCTATAA TCATCCAAAA GCAGGAGAGA AGTACGGGAG TGGGACTGAT 
AGCGACTATG ACAACTCTCA AACATATGAC GTCTCCATTG GTGTGGGCCG CAGCAGTGAG 
GAGGACAGCA GGGGGGATGT AGACGACACC GAGGGCGATC CTGACCCCAC ACTGCCCTGC 
ACAGAGGACG TCATCCTTAA AACTGAACAG GTCACCAAAA ACATCCAGGA GCTCCTGAGA 
GCTGCTCAAG AGTTCAAGCA CGACAGTTTT GTGCCATGCT CAGAGAAAAT CCACTTAGCT 
GTGACAGAAA TGGCCTCGCT GTTTCCCAAG AGACCAGCGC TGGATGCAGT ACGTTCTTCC 
TTGAAGCTCC TGGCAGCAAG TGCGTCACGT TTGCAGGTGG AGTGTCGTAA AGCGGCGCCT 
TCGGACTCGT CGGCCAGTAC AGTGGACTAC CAGCTTCTTA CCCAGCAGGT CATCCAGTGC 
GCATACGACA TTGCCAAAGC CGCCAAGCAG CTGGTCACCA TCACCACCCG CGAAAAAAAA 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_697386.4
CDS	478..2763
Translation	MSRKVQRSEVCADCSAPDPGWSSINRGVLICDECCSVHRSLGRHISIVKHLRHSGWPPSLLQMVQTLASNGANSIWEHSLLDPAQVQSGRRKPNPQDKVHPTKSEFIRAKYQMLAFVHKLPCRDDDGVTTKDLSKQLHSSVRTGSLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQVLQAELLVVYGADPGAPDINGRTPIDYARQAGHVELAERLVECQYELTDRLAFYLCGRRPDHKNGHYIIPQMADRARPKCPTQSLDLSELAKAAKKKLQALNNRLFEELAMDVYDEVDRRENDAVWLTTQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSDAKRRQHGKGLTSPTDPLDLSQVDDDQHDYDSVASDEDTDSELTAQNNNNTQRNNRAKSMDSSDLSDGPITLQEYLEVKKALASSEAKVQQLMKVNNNLSEELRRLQKEISRMQTENSALRGAQAGSGATMTGGGVPGHWPDRVRGMGGGGGLSGDSSLIAPSSTPHRRDRQAFSMYEPVGTTSKVLSPALDPLTCRLQPLSPVRKGAPAGPTPYGGAHLSASTDGRYNHPKAGEKYGSGTDSDYDNSQTYDVSIGVGRSSEEDSRGDVDDTEGDPDPTLPCTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAVTEMASLFPKRPALDAVRSSLKLLAASASRLQVECRKAAPSDSSASTVDYQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_692294.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_692294.8

ORF Insert Sequence:

ATGTCCAGGA AAGTCCAGAG AAGCGAGGTG TGCGCCGACT GCAGTGCACC AGACCCGGGC 
TGGAGCAGTA TAAACCGAGG AGTCCTGATC TGCGATGAGT GCTGTTCTGT ACACCGGAGC 
CTGGGCCGTC ACATTTCCAT CGTCAAACAC TTGAGACACA GCGGCTGGCC TCCTTCCTTA 
CTGCAGATGG TGCAGACCCT GGCCAGTAAC GGCGCTAACT CGATCTGGGA ACACTCTCTG 
CTGGATCCAG CACAAGTTCA GAGTGGCCGC AGGAAACCAA ACCCACAAGA TAAAGTCCAC 
CCCACAAAGT CAGAGTTCAT TCGCGCCAAG TACCAGATGT TAGCCTTTGT CCATAAGTTG 
CCCTGTCGTG ATGACGACGG AGTCACTACT AAGGACCTTA GCAAGCAACT GCACTCAAGT 
GTGCGGACAG GAAGTCTGGA GACATGTTTA CGGCTGCTGT CACTGGGGGC ACAAGCCAAC 
TTCTTCCACC CGGAAAAGGG CACAACCCCT CTCCATGTGG CTGCCAAAGC GGGACAGGTT 
CTCCAGGCTG AGCTGCTGGT TGTGTATGGA GCGGATCCGG GAGCTCCAGA TATCAATGGC 
CGAACACCCA TAGACTATGC ACGGCAAGCG GGTCACGTGG AGTTGGCCGA ACGCTTGGTG 
GAGTGTCAGT ATGAGCTCAC GGACAGACTG GCCTTCTACC TGTGTGGACG TCGCCCAGAT 
CATAAGAATG GTCATTATAT CATCCCTCAG ATGGCAGACA GAGCTCGCCC TAAGTGCCCG 
ACGCAGAGCC TGGACCTCTC TGAACTGGCC AAGGCAGCCA AGAAGAAGCT CCAAGCGTTA 
AATAATCGTT TGTTCGAGGA GCTGGCTATG GACGTGTACG ATGAAGTGGA CCGTAGAGAG 
AATGATGCAG TGTGGCTGAC AACTCAGAAC CACAGTACGC TGGTGACCGA ACGGAGCGCT 
GTCCCATTCT TACCCGTCAA TCCTGAATAC TCAGCGACAC GCAACCAGGG GCGGCAGAAA 
TTAGCACGCT TCAATGCGCG GGAATTCGCC ACCCTCATTA TCGACATCCT CAGCGACGCC 
AAGCGACGGC AACATGGAAA AGGCCTGACG AGCCCCACAG ACCCGCTGGA TCTGAGTCAG 
GTGGACGATG ACCAGCACGA TTATGACAGC GTAGCGTCTG ATGAAGACAC AGACAGCGAG 
CTGACCGCAC AGAATAATAA CAACACCCAA CGCAACAACC GGGCCAAGAG TATGGACTCG 
TCAGATCTGT CAGATGGACC AATCACACTA CAGGAGTATC TGGAGGTGAA GAAAGCACTG 
GCTTCCTCTG AGGCTAAAGT TCAGCAGCTC ATGAAGGTCA ACAACAACCT GAGTGAGGAG 
CTGCGGAGGC TGCAGAAAGA GATCTCGCGG ATGCAGACGG AGAACAGCGC GCTGCGGGGG 
GCCCAGGCTG GGTCGGGGGC CACCATGACT GGAGGTGGTG TTCCAGGACA CTGGCCTGAT 
AGAGTAAGGG GTATGGGTGG TGGAGGAGGG CTAAGTGGAG ATTCCAGCCT AATTGCACCC 
TCGTCCACCC CCCACCGACG GGACAGGCAG GCTTTCTCTA TGTATGAGCC TGTGGGCACC 
ACCTCAAAAG TCCTCAGCCC AGCCCTGGAC CCCCTGACTT GCCGCCTGCA GCCTCTCAGC 
CCTGTGCGAA AGGGCGCTCC AGCGGGTCCC ACCCCATACG GAGGAGCGCA CCTGTCAGCC 
TCCACTGACG GACGCTATAA TCATCCAAAA GCAGGAGAGA AGTACGGGAG TGGGACTGAT 
AGCGACTATG ACAACTCTCA AACATATGAC GTCTCCATTG GTGTGGGCCG CAGCAGTGAG 
GAGGACAGCA GGGGGGATGT AGACGACACC GAGGGCGATC CTGACCCCAC ACTGCCCTGC 
ACAGAGGACG TCATCCTTAA AACTGAACAG GTCACCAAAA ACATCCAGGA GCTCCTGAGA 
GCTGCTCAAG AGTTCAAGCA CGACAGTTTT GTGCCATGCT CAGAGAAAAT CCACTTAGCT 
GTGACAGAAA TGGCCTCGCT GTTTCCCAAG AGACCAGCGC TGGATGCAGT ACGTTCTTCC 
TTGAAGCTCC TGGCAGCAAG TGCGTCACGT TTGCAGGTGG AGTGTCGTAA AGCGGCGCCT 
TCGGACTCGT CGGCCAGTAC AGTGGACTAC CAGCTTCTTA CCCAGCAGGT CATCCAGTGC 
GCATACGACA TTGCCAAAGC CGCCAAGCAG CTGGTCACCA TCACCACCCG CGAAAAAAAA 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

git1 ( XM_005157670.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157727.1 Danio rerio G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa20578
Clone ID Related Accession (Same CDS sequence)	XM_005157670.2 , XM_005157670.3
Accession Version	XM_005157670.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ARF GTPase-activating protein GIT1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005157670.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGGA AAGTCCAGAG AAGCGAGGTG TGCGCCGACT GCAGTGCACC AGACCCGGGC 
TGGAGCAGTA TAAACCGAGG AGTCCTGATC TGCGATGAGT GCTGTTCTGT ACACCGGAGC 
CTGGGCCGTC ACATTTCCAT CGTCAAACAC TTGAGACACA GCGGCTGGCC TCCTTCCTTA 
CTGCAGATGG TGCAGACCCT GGCCAGTAAC GGCGCTAACT CGATCTGGGA ACACTCTCTG 
CTGGATCCAG CACAAGTTCA GAGTGGCCGC AGGAAACCAA ACCCACAAGA TAAAGTCCAC 
CCCACAAAGT CAGAGTTCAT TCGCGCCAAG TACCAGATGT TAGCCTTTGT CCATAAGTTG 
CCCTGTCGTG ATGACGACGG AGTCACTACT AAGGACCTTA GCAAGCAACT GCACTCAAGT 
GTGCGGACAG GAAGTCTGGA GACATGTTTA CGGCTGCTGT CACTGGGGGC ACAAGCCAAC 
TTCTTCCACC CGGAAAAGGG CACAACCCCT CTCCATGTGG CTGCCAAAGC GGGACAGGTT 
CTCCAGGCTG AGCTGCTGGT TGTGTATGGA GCGGATCCGG GAGCTCCAGA TATCAATGGC 
CGAACACCCA TAGACTATGC ACGGCAAGCG GGTCACGTGG AGTTGGCCGA ACGCTTGGTG 
GAGTGTCAGT ATGAGCTCAC GGACAGACTG GCCTTCTACC TGTGTGGACG TCGCCCAGAT 
CATAAGAATG GTCATTATAT CATCCCTCAG ATGGCAGACA GCCTGGACCT CTCTGAACTG 
GCCAAGGCAG CCAAGAAGAA GCTCCAAGCG TTAAATAATC GTTTGTTCGA GGAGCTGGCT 
ATGGACGTGT ACGATGAAGT GGACCGTAGA GAGAATGATG CAGTGTGGCT GACAACTCAG 
AACCACAGTA CGCTGGTGAC CGAACGGAGC GCTGTCCCAT TCTTACCCGT CAATCCTGAA 
TACTCAGCGA CACGCAACCA GGGGCGGCAG AAATTAGCAC GCTTCAATGC GCGGGAATTC 
GCCACCCTCA TTATCGACAT CCTCAGCGAC GCCAAGCGAC GGCAACATGG AAAAGGCCTG 
ACGAGCCCCA CAGACCCGCT GGATCTGAGT CAGGTGGACG ATGACCAGCA CGATTATGAC 
AGCGTAGCGT CTGATGAAGA CACAGACAGC GAGCTGACCG CACAGAATAA TAACAACACC 
CAACGCAACA ACCGGGCCAA GAGTATGGAC TCGTCAGATC TGTCAGATGG ACCAATCACA 
CTACAGGAGT ATCTGGAGGT GAAGAAAGCA CTGGCTTCCT CTGAGGCTAA AGTTCAGCAG 
CTCATGAAGG TCAACAACAA CCTGAGTGAG GAGCTGCGGA GGCTGCAGAA AGAGATCTCG 
CGGATGCAGA CGGAGAACAG CGCGCTGCGG GGGGCCCAGG CTGGGTCGGG GGCCACCATG 
ACTGGAGGTG GTGTTCCAGG ACACTGGCCT GATAGAGTAA GGGGTATGGG TGGTGGAGGA 
GGGCTAAGTG GAGATTCCAG CCTAATTGCA CCCTCGTCCA CCCCCCACCG ACGGGACAGG 
CAGGCTTTCT CTATGTATGA GCCTGTGGGC ACCACCTCAA AAGTCCTCAG CCCAGCCCTG 
GACCCCCTGA CTTGCCGCCT GCAGCCTCTC AGCCCTGTGC GAAAGGGCGC TCCAGCGGGT 
CCCACCCCAT ACGGAGGAGC GCACCTGTCA GCCTCCACTG ACGGACGCTA TAATCATCCA 
AAAGCAGGAG AGAAGTACGG GAGTGGGACT GATAGCGACT ATGACAACTC TCAAACATAT 
GACGTCTCCA TTGGTGTGGG CCGCAGCAGT GAGGAGGACA GCAGGGGGGA TGTAGACGAC 
ACCGAGGGCG ATCCTGACCC CACACTGCCC TGCACAGAGG ACGTCATCCT TAAAACTGAA 
CAGGTCACCA AAAACATCCA GGAGCTCCTG AGAGCTGCTC AAGAGTTCAA GCACGACAGT 
TTTGTGCCAT GCTCAGAGAA AATCCACTTA GCTGTGACAG AAATGGCCTC GCTGTTTCCC 
AAGAGACCAG CGCTGGATGC AGTACGTTCT TCCTTGAAGC TCCTGGCAGC AAGTGCGTCA 
CGTTTGCAGG TGGAGTGTCG TAAAGCGGCG CCTTCGGACT CGTCGGCCAG TACAGTGGAC 
TACCAGCTTC TTACCCAGCA GGTCATCCAG TGCGCATACG ACATTGCCAA AGCCGCCAAG 
CAGCTGGTCA CCATCACCAC CCGCGAAAAA AAACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157727.1
CDS	467..2725
Translation	MSRKVQRSEVCADCSAPDPGWSSINRGVLICDECCSVHRSLGRHISIVKHLRHSGWPPSLLQMVQTLASNGANSIWEHSLLDPAQVQSGRRKPNPQDKVHPTKSEFIRAKYQMLAFVHKLPCRDDDGVTTKDLSKQLHSSVRTGSLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQVLQAELLVVYGADPGAPDINGRTPIDYARQAGHVELAERLVECQYELTDRLAFYLCGRRPDHKNGHYIIPQMADSLDLSELAKAAKKKLQALNNRLFEELAMDVYDEVDRRENDAVWLTTQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSDAKRRQHGKGLTSPTDPLDLSQVDDDQHDYDSVASDEDTDSELTAQNNNNTQRNNRAKSMDSSDLSDGPITLQEYLEVKKALASSEAKVQQLMKVNNNLSEELRRLQKEISRMQTENSALRGAQAGSGATMTGGGVPGHWPDRVRGMGGGGGLSGDSSLIAPSSTPHRRDRQAFSMYEPVGTTSKVLSPALDPLTCRLQPLSPVRKGAPAGPTPYGGAHLSASTDGRYNHPKAGEKYGSGTDSDYDNSQTYDVSIGVGRSSEEDSRGDVDDTEGDPDPTLPCTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAVTEMASLFPKRPALDAVRSSLKLLAASASRLQVECRKAAPSDSSASTVDYQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_005157670.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157670.2

ORF Insert Sequence:

ATGTCCAGGA AAGTCCAGAG AAGCGAGGTG TGCGCCGACT GCAGTGCACC AGACCCGGGC 
TGGAGCAGTA TAAACCGAGG AGTCCTGATC TGCGATGAGT GCTGTTCTGT ACACCGGAGC 
CTGGGCCGTC ACATTTCCAT CGTCAAACAC TTGAGACACA GCGGCTGGCC TCCTTCCTTA 
CTGCAGATGG TGCAGACCCT GGCCAGTAAC GGCGCTAACT CGATCTGGGA ACACTCTCTG 
CTGGATCCAG CACAAGTTCA GAGTGGCCGC AGGAAACCAA ACCCACAAGA TAAAGTCCAC 
CCCACAAAGT CAGAGTTCAT TCGCGCCAAG TACCAGATGT TAGCCTTTGT CCATAAGTTG 
CCCTGTCGTG ATGACGACGG AGTCACTACT AAGGACCTTA GCAAGCAACT GCACTCAAGT 
GTGCGGACAG GAAGTCTGGA GACATGTTTA CGGCTGCTGT CACTGGGGGC ACAAGCCAAC 
TTCTTCCACC CGGAAAAGGG CACAACCCCT CTCCATGTGG CTGCCAAAGC GGGACAGGTT 
CTCCAGGCTG AGCTGCTGGT TGTGTATGGA GCGGATCCGG GAGCTCCAGA TATCAATGGC 
CGAACACCCA TAGACTATGC ACGGCAAGCG GGTCACGTGG AGTTGGCCGA ACGCTTGGTG 
GAGTGTCAGT ATGAGCTCAC GGACAGACTG GCCTTCTACC TGTGTGGACG TCGCCCAGAT 
CATAAGAATG GTCATTATAT CATCCCTCAG ATGGCAGACA GCCTGGACCT CTCTGAACTG 
GCCAAGGCAG CCAAGAAGAA GCTCCAAGCG TTAAATAATC GTTTGTTCGA GGAGCTGGCT 
ATGGACGTGT ACGATGAAGT GGACCGTAGA GAGAATGATG CAGTGTGGCT GACAACTCAG 
AACCACAGTA CGCTGGTGAC CGAACGGAGC GCTGTCCCAT TCTTACCCGT CAATCCTGAA 
TACTCAGCGA CACGCAACCA GGGGCGGCAG AAATTAGCAC GCTTCAATGC GCGGGAATTC 
GCCACCCTCA TTATCGACAT CCTCAGCGAC GCCAAGCGAC GGCAACATGG AAAAGGCCTG 
ACGAGCCCCA CAGACCCGCT GGATCTGAGT CAGGTGGACG ATGACCAGCA CGATTATGAC 
AGCGTAGCGT CTGATGAAGA CACAGACAGC GAGCTGACCG CACAGAATAA TAACAACACC 
CAACGCAACA ACCGGGCCAA GAGTATGGAC TCGTCAGATC TGTCAGATGG ACCAATCACA 
CTACAGGAGT ATCTGGAGGT GAAGAAAGCA CTGGCTTCCT CTGAGGCTAA AGTTCAGCAG 
CTCATGAAGG TCAACAACAA CCTGAGTGAG GAGCTGCGGA GGCTGCAGAA AGAGATCTCG 
CGGATGCAGA CGGAGAACAG CGCGCTGCGG GGGGCCCAGG CTGGGTCGGG GGCCACCATG 
ACTGGAGGTG GTGTTCCAGG ACACTGGCCT GATAGAGTAA GGGGTATGGG TGGTGGAGGA 
GGGCTAAGTG GAGATTCCAG CCTAATTGCA CCCTCGTCCA CCCCCCACCG ACGGGACAGG 
CAGGCTTTCT CTATGTATGA GCCTGTGGGC ACCACCTCAA AAGTCCTCAG CCCAGCCCTG 
GACCCCCTGA CTTGCCGCCT GCAGCCTCTC AGCCCTGTGC GAAAGGGCGC TCCAGCGGGT 
CCCACCCCAT ACGGAGGAGC GCACCTGTCA GCCTCCACTG ACGGACGCTA TAATCATCCA 
AAAGCAGGAG AGAAGTACGG GAGTGGGACT GATAGCGACT ATGACAACTC TCAAACATAT 
GACGTCTCCA TTGGTGTGGG CCGCAGCAGT GAGGAGGACA GCAGGGGGGA TGTAGACGAC 
ACCGAGGGCG ATCCTGACCC CACACTGCCC TGCACAGAGG ACGTCATCCT TAAAACTGAA 
CAGGTCACCA AAAACATCCA GGAGCTCCTG AGAGCTGCTC AAGAGTTCAA GCACGACAGT 
TTTGTGCCAT GCTCAGAGAA AATCCACTTA GCTGTGACAG AAATGGCCTC GCTGTTTCCC 
AAGAGACCAG CGCTGGATGC AGTACGTTCT TCCTTGAAGC TCCTGGCAGC AAGTGCGTCA 
CGTTTGCAGG TGGAGTGTCG TAAAGCGGCG CCTTCGGACT CGTCGGCCAG TACAGTGGAC 
TACCAGCTTC TTACCCAGCA GGTCATCCAG TGCGCATACG ACATTGCCAA AGCCGCCAAG 
CAGCTGGTCA CCATCACCAC CCGCGAAAAA AAACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

git1 ( XM_005157670.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157727.1 Danio rerio G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa20578
Clone ID Related Accession (Same CDS sequence)	XM_005157670.2 , XM_005157670.3
Accession Version	XM_005157670.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2259bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ARF GTPase-activating protein GIT1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157670.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGGA AAGTCCAGAG AAGCGAGGTG TGCGCCGACT GCAGTGCACC AGACCCGGGC 
TGGAGCAGTA TAAACCGAGG AGTCCTGATC TGCGATGAGT GCTGTTCTGT ACACCGGAGC 
CTGGGCCGTC ACATTTCCAT CGTCAAACAC TTGAGACACA GCGGCTGGCC TCCTTCCTTA 
CTGCAGATGG TGCAGACCCT GGCCAGTAAC GGCGCTAACT CGATCTGGGA ACACTCTCTG 
CTGGATCCAG CACAAGTTCA GAGTGGCCGC AGGAAACCAA ACCCACAAGA TAAAGTCCAC 
CCCACAAAGT CAGAGTTCAT TCGCGCCAAG TACCAGATGT TAGCCTTTGT CCATAAGTTG 
CCCTGTCGTG ATGACGACGG AGTCACTACT AAGGACCTTA GCAAGCAACT GCACTCAAGT 
GTGCGGACAG GAAGTCTGGA GACATGTTTA CGGCTGCTGT CACTGGGGGC ACAAGCCAAC 
TTCTTCCACC CGGAAAAGGG CACAACCCCT CTCCATGTGG CTGCCAAAGC GGGACAGGTT 
CTCCAGGCTG AGCTGCTGGT TGTGTATGGA GCGGATCCGG GAGCTCCAGA TATCAATGGC 
CGAACACCCA TAGACTATGC ACGGCAAGCG GGTCACGTGG AGTTGGCCGA ACGCTTGGTG 
GAGTGTCAGT ATGAGCTCAC GGACAGACTG GCCTTCTACC TGTGTGGACG TCGCCCAGAT 
CATAAGAATG GTCATTATAT CATCCCTCAG ATGGCAGACA GCCTGGACCT CTCTGAACTG 
GCCAAGGCAG CCAAGAAGAA GCTCCAAGCG TTAAATAATC GTTTGTTCGA GGAGCTGGCT 
ATGGACGTGT ACGATGAAGT GGACCGTAGA GAGAATGATG CAGTGTGGCT GACAACTCAG 
AACCACAGTA CGCTGGTGAC CGAACGGAGC GCTGTCCCAT TCTTACCCGT CAATCCTGAA 
TACTCAGCGA CACGCAACCA GGGGCGGCAG AAATTAGCAC GCTTCAATGC GCGGGAATTC 
GCCACCCTCA TTATCGACAT CCTCAGCGAC GCCAAGCGAC GGCAACATGG AAAAGGCCTG 
ACGAGCCCCA CAGACCCGCT GGATCTGAGT CAGGTGGACG ATGACCAGCA CGATTATGAC 
AGCGTAGCGT CTGATGAAGA CACAGACAGC GAGCTGACCG CACAGAATAA TAACAACACC 
CAACGCAACA ACCGGGCCAA GAGTATGGAC TCGTCAGATC TGTCAGATGG ACCAATCACA 
CTACAGGAGT ATCTGGAGGT GAAGAAAGCA CTGGCTTCCT CTGAGGCTAA AGTTCAGCAG 
CTCATGAAGG TCAACAACAA CCTGAGTGAG GAGCTGCGGA GGCTGCAGAA AGAGATCTCG 
CGGATGCAGA CGGAGAACAG CGCGCTGCGG GGGGCCCAGG CTGGGTCGGG GGCCACCATG 
ACTGGAGGTG GTGTTCCAGG ACACTGGCCT GATAGAGTAA GGGGTATGGG TGGTGGAGGA 
GGGCTAAGTG GAGATTCCAG CCTAATTGCA CCCTCGTCCA CCCCCCACCG ACGGGACAGG 
CAGGCTTTCT CTATGTATGA GCCTGTGGGC ACCACCTCAA AAGTCCTCAG CCCAGCCCTG 
GACCCCCTGA CTTGCCGCCT GCAGCCTCTC AGCCCTGTGC GAAAGGGCGC TCCAGCGGGT 
CCCACCCCAT ACGGAGGAGC GCACCTGTCA GCCTCCACTG ACGGACGCTA TAATCATCCA 
AAAGCAGGAG AGAAGTACGG GAGTGGGACT GATAGCGACT ATGACAACTC TCAAACATAT 
GACGTCTCCA TTGGTGTGGG CCGCAGCAGT GAGGAGGACA GCAGGGGGGA TGTAGACGAC 
ACCGAGGGCG ATCCTGACCC CACACTGCCC TGCACAGAGG ACGTCATCCT TAAAACTGAA 
CAGGTCACCA AAAACATCCA GGAGCTCCTG AGAGCTGCTC AAGAGTTCAA GCACGACAGT 
TTTGTGCCAT GCTCAGAGAA AATCCACTTA GCTGTGACAG AAATGGCCTC GCTGTTTCCC 
AAGAGACCAG CGCTGGATGC AGTACGTTCT TCCTTGAAGC TCCTGGCAGC AAGTGCGTCA 
CGTTTGCAGG TGGAGTGTCG TAAAGCGGCG CCTTCGGACT CGTCGGCCAG TACAGTGGAC 
TACCAGCTTC TTACCCAGCA GGTCATCCAG TGCGCATACG ACATTGCCAA AGCCGCCAAG 
CAGCTGGTCA CCATCACCAC CCGCGAAAAA AAACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157727.1
CDS	478..2736
Translation	MSRKVQRSEVCADCSAPDPGWSSINRGVLICDECCSVHRSLGRHISIVKHLRHSGWPPSLLQMVQTLASNGANSIWEHSLLDPAQVQSGRRKPNPQDKVHPTKSEFIRAKYQMLAFVHKLPCRDDDGVTTKDLSKQLHSSVRTGSLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQVLQAELLVVYGADPGAPDINGRTPIDYARQAGHVELAERLVECQYELTDRLAFYLCGRRPDHKNGHYIIPQMADSLDLSELAKAAKKKLQALNNRLFEELAMDVYDEVDRRENDAVWLTTQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSDAKRRQHGKGLTSPTDPLDLSQVDDDQHDYDSVASDEDTDSELTAQNNNNTQRNNRAKSMDSSDLSDGPITLQEYLEVKKALASSEAKVQQLMKVNNNLSEELRRLQKEISRMQTENSALRGAQAGSGATMTGGGVPGHWPDRVRGMGGGGGLSGDSSLIAPSSTPHRRDRQAFSMYEPVGTTSKVLSPALDPLTCRLQPLSPVRKGAPAGPTPYGGAHLSASTDGRYNHPKAGEKYGSGTDSDYDNSQTYDVSIGVGRSSEEDSRGDVDDTEGDPDPTLPCTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAVTEMASLFPKRPALDAVRSSLKLLAASASRLQVECRKAAPSDSSASTVDYQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_005157670.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157670.3

ORF Insert Sequence:

ATGTCCAGGA AAGTCCAGAG AAGCGAGGTG TGCGCCGACT GCAGTGCACC AGACCCGGGC 
TGGAGCAGTA TAAACCGAGG AGTCCTGATC TGCGATGAGT GCTGTTCTGT ACACCGGAGC 
CTGGGCCGTC ACATTTCCAT CGTCAAACAC TTGAGACACA GCGGCTGGCC TCCTTCCTTA 
CTGCAGATGG TGCAGACCCT GGCCAGTAAC GGCGCTAACT CGATCTGGGA ACACTCTCTG 
CTGGATCCAG CACAAGTTCA GAGTGGCCGC AGGAAACCAA ACCCACAAGA TAAAGTCCAC 
CCCACAAAGT CAGAGTTCAT TCGCGCCAAG TACCAGATGT TAGCCTTTGT CCATAAGTTG 
CCCTGTCGTG ATGACGACGG AGTCACTACT AAGGACCTTA GCAAGCAACT GCACTCAAGT 
GTGCGGACAG GAAGTCTGGA GACATGTTTA CGGCTGCTGT CACTGGGGGC ACAAGCCAAC 
TTCTTCCACC CGGAAAAGGG CACAACCCCT CTCCATGTGG CTGCCAAAGC GGGACAGGTT 
CTCCAGGCTG AGCTGCTGGT TGTGTATGGA GCGGATCCGG GAGCTCCAGA TATCAATGGC 
CGAACACCCA TAGACTATGC ACGGCAAGCG GGTCACGTGG AGTTGGCCGA ACGCTTGGTG 
GAGTGTCAGT ATGAGCTCAC GGACAGACTG GCCTTCTACC TGTGTGGACG TCGCCCAGAT 
CATAAGAATG GTCATTATAT CATCCCTCAG ATGGCAGACA GCCTGGACCT CTCTGAACTG 
GCCAAGGCAG CCAAGAAGAA GCTCCAAGCG TTAAATAATC GTTTGTTCGA GGAGCTGGCT 
ATGGACGTGT ACGATGAAGT GGACCGTAGA GAGAATGATG CAGTGTGGCT GACAACTCAG 
AACCACAGTA CGCTGGTGAC CGAACGGAGC GCTGTCCCAT TCTTACCCGT CAATCCTGAA 
TACTCAGCGA CACGCAACCA GGGGCGGCAG AAATTAGCAC GCTTCAATGC GCGGGAATTC 
GCCACCCTCA TTATCGACAT CCTCAGCGAC GCCAAGCGAC GGCAACATGG AAAAGGCCTG 
ACGAGCCCCA CAGACCCGCT GGATCTGAGT CAGGTGGACG ATGACCAGCA CGATTATGAC 
AGCGTAGCGT CTGATGAAGA CACAGACAGC GAGCTGACCG CACAGAATAA TAACAACACC 
CAACGCAACA ACCGGGCCAA GAGTATGGAC TCGTCAGATC TGTCAGATGG ACCAATCACA 
CTACAGGAGT ATCTGGAGGT GAAGAAAGCA CTGGCTTCCT CTGAGGCTAA AGTTCAGCAG 
CTCATGAAGG TCAACAACAA CCTGAGTGAG GAGCTGCGGA GGCTGCAGAA AGAGATCTCG 
CGGATGCAGA CGGAGAACAG CGCGCTGCGG GGGGCCCAGG CTGGGTCGGG GGCCACCATG 
ACTGGAGGTG GTGTTCCAGG ACACTGGCCT GATAGAGTAA GGGGTATGGG TGGTGGAGGA 
GGGCTAAGTG GAGATTCCAG CCTAATTGCA CCCTCGTCCA CCCCCCACCG ACGGGACAGG 
CAGGCTTTCT CTATGTATGA GCCTGTGGGC ACCACCTCAA AAGTCCTCAG CCCAGCCCTG 
GACCCCCTGA CTTGCCGCCT GCAGCCTCTC AGCCCTGTGC GAAAGGGCGC TCCAGCGGGT 
CCCACCCCAT ACGGAGGAGC GCACCTGTCA GCCTCCACTG ACGGACGCTA TAATCATCCA 
AAAGCAGGAG AGAAGTACGG GAGTGGGACT GATAGCGACT ATGACAACTC TCAAACATAT 
GACGTCTCCA TTGGTGTGGG CCGCAGCAGT GAGGAGGACA GCAGGGGGGA TGTAGACGAC 
ACCGAGGGCG ATCCTGACCC CACACTGCCC TGCACAGAGG ACGTCATCCT TAAAACTGAA 
CAGGTCACCA AAAACATCCA GGAGCTCCTG AGAGCTGCTC AAGAGTTCAA GCACGACAGT 
TTTGTGCCAT GCTCAGAGAA AATCCACTTA GCTGTGACAG AAATGGCCTC GCTGTTTCCC 
AAGAGACCAG CGCTGGATGC AGTACGTTCT TCCTTGAAGC TCCTGGCAGC AAGTGCGTCA 
CGTTTGCAGG TGGAGTGTCG TAAAGCGGCG CCTTCGGACT CGTCGGCCAG TACAGTGGAC 
TACCAGCTTC TTACCCAGCA GGTCATCCAG TGCGCATACG ACATTGCCAA AGCCGCCAAG 
CAGCTGGTCA CCATCACCAC CCGCGAAAAA AAACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

git1 ( XM_009291756.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290031.1 Danio rerio G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa45617
Clone ID Related Accession (Same CDS sequence)	XM_009291756.1 , XM_009291756.2
Accession Version	XM_009291756.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ARF GTPase-activating protein GIT1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGGA AAGTCCAGAG AAGCGAGGTG TGCGCCGACT GCAGTGCACC AGACCCGGGC 
TGGAGCAGTA TAAACCGAGG AGTCCTGATC TGCGATGAGT GCTGTTCTGT ACACCGGAGC 
CTGGGCCGTC ACATTTCCAT CGTCAAACAC TTGAGACACA GCGGCTGGCC TCCTTCCTTA 
CTGCAGATGG TGCAGACCCT GGCCAGTAAC GGCGCTAACT CGATCTGGGA ACACTCTCTG 
CTGGATCCAG CACAAGTTCA GAGTGGCCGC AGGAAACCAA ACCCACAAGA TAAAGTCCAC 
CCCACAAAGT CAGAGTTCAT TCGCGCCAAG TACCAGATGT TAGCCTTTGT CCATAAGTTG 
CCCTGTCGTG ATGACGACGG AGTCACTACT AAGGACCTTA GCAAGCAACT GCACTCAAGT 
GTGCGGACAG GAAGTCTGGA GACATGTTTA CGGCTGCTGT CACTGGGGGC ACAAGCCAAC 
TTCTTCCACC CGGAAAAGGG CACAACCCCT CTCCATGTGG CTGCCAAAGC GGGACAGGTT 
CTCCAGGCTG AGCTGCTGGT TGTGTATGGA GCGGATCCGG GAGCTCCAGA TATCAATGGC 
CGAACACCCA TAGACTATGC ACGGCAAGCG GGTCACGTGG AGTTGGCCGA ACGCTTGGTG 
GAGTGTCAGT ATGAGCTCAC GGACAGACTG GCCTTCTACC TGTGTGGACG TCGCCCAGAT 
CATAAGAATG GTCATTATAT CATCCCTCAG ATGGCAGACA GAGCTCGCCC TAAGTGCCCG 
ACGCAGAGCC TGGACCTCTC TGAACTGGCC AAGGCAGCCA AGAAGAAGCT CCAAGCGTTA 
AATAATCGTT TGTTCGAGGA GCTGGCTATG GACGTGTACG ATGAAGTGGA CCGTAGAGAG 
AATGATGCAG TGTGGCTGAC AACTCAGAAC CACAGTACGC TGGTGACCGA ACGGAGCGCT 
GTCCCATTCT TACCCGTCAA TCCTGAATAC TCAGCGACAC GCAACCAGGG GCGGCAGAAA 
TTAGCACGCT TCAATGCGCG GGAATTCGCC ACCCTCATTA TCGACATCCT CAGCGACGCC 
AAGCGACGGC AACATGGAAA AGGCCTGACG AGCCCCACAG ACCCGCTGGA TCTGAGTCAG 
GTGGACGATG ACCAGCACGA TTATGACAGC GTAGCGTCTG ATGAAGACAC AGACAGCGAG 
CTGACCGCAC AGAATAATAA CAACACCCAA CGCAACAACC GGGCCAAGAG TATGGACTCG 
TCAGATCTGT CAGATGGACC AATCACACTA CAGGAGTATC TGGAGGTGAA GAAAGCACTG 
GCTTCCTCTG AGGCTAAAGT TCAGCAGCTC ATGAAGGTCA ACAACAACCT GAGTGAGGAG 
CTGCGGAGGC TGCAGAAAGA GGTGCGAAAG GGCGCTCCAG CGGGTCCCAC CCCATACGGA 
GGAGCGCACC TGTCAGCCTC CACTGACGGA CGCTATAATC ATCCAAAAGC AGGAGAGAAG 
TACGGGAGTG GGACTGATAG CGACTATGAC AACTCTCAAA CATATGACGT CTCCATTGGT 
GTGGGCCGCA GCAGTGAGGA GGACAGCAGG GGGGATGTAG ACGACACCGA GGGCGATCCT 
GACCCCACAC TGCCCTGCAC AGAGGACGTC ATCCTTAAAA CTGAACAGGT CACCAAAAAC 
ATCCAGGAGC TCCTGAGAGC TGCTCAAGAG TTCAAGCACG ACAGTTTTGT GCCATGCTCA 
GAGAAAATCC ACTTAGCTGT GACAGAAATG GCCTCGCTGT TTCCCAAGAG ACCAGCGCTG 
GATGCAGTAC GTTCTTCCTT GAAGCTCCTG GCAGCAAGTG CGTCACGTTT GCAGGTGGAG 
TGTCGTAAAG CGGCGCCTTC GGACTCGTCG GCCAGTACAG TGGACTACCA GCTTCTTACC 
CAGCAGGTCA TCCAGTGCGC ATACGACATT GCCAAAGCCG CCAAGCAGCT GGTCACCATC 
ACCACCCGCG AAAAAAAACA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290031.1
CDS	467..2470
Translation	MSRKVQRSEVCADCSAPDPGWSSINRGVLICDECCSVHRSLGRHISIVKHLRHSGWPPSLLQMVQTLASNGANSIWEHSLLDPAQVQSGRRKPNPQDKVHPTKSEFIRAKYQMLAFVHKLPCRDDDGVTTKDLSKQLHSSVRTGSLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQVLQAELLVVYGADPGAPDINGRTPIDYARQAGHVELAERLVECQYELTDRLAFYLCGRRPDHKNGHYIIPQMADRARPKCPTQSLDLSELAKAAKKKLQALNNRLFEELAMDVYDEVDRRENDAVWLTTQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSDAKRRQHGKGLTSPTDPLDLSQVDDDQHDYDSVASDEDTDSELTAQNNNNTQRNNRAKSMDSSDLSDGPITLQEYLEVKKALASSEAKVQQLMKVNNNLSEELRRLQKEVRKGAPAGPTPYGGAHLSASTDGRYNHPKAGEKYGSGTDSDYDNSQTYDVSIGVGRSSEEDSRGDVDDTEGDPDPTLPCTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAVTEMASLFPKRPALDAVRSSLKLLAASASRLQVECRKAAPSDSSASTVDYQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_009291756.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291756.1

ORF Insert Sequence:

ATGTCCAGGA AAGTCCAGAG AAGCGAGGTG TGCGCCGACT GCAGTGCACC AGACCCGGGC 
TGGAGCAGTA TAAACCGAGG AGTCCTGATC TGCGATGAGT GCTGTTCTGT ACACCGGAGC 
CTGGGCCGTC ACATTTCCAT CGTCAAACAC TTGAGACACA GCGGCTGGCC TCCTTCCTTA 
CTGCAGATGG TGCAGACCCT GGCCAGTAAC GGCGCTAACT CGATCTGGGA ACACTCTCTG 
CTGGATCCAG CACAAGTTCA GAGTGGCCGC AGGAAACCAA ACCCACAAGA TAAAGTCCAC 
CCCACAAAGT CAGAGTTCAT TCGCGCCAAG TACCAGATGT TAGCCTTTGT CCATAAGTTG 
CCCTGTCGTG ATGACGACGG AGTCACTACT AAGGACCTTA GCAAGCAACT GCACTCAAGT 
GTGCGGACAG GAAGTCTGGA GACATGTTTA CGGCTGCTGT CACTGGGGGC ACAAGCCAAC 
TTCTTCCACC CGGAAAAGGG CACAACCCCT CTCCATGTGG CTGCCAAAGC GGGACAGGTT 
CTCCAGGCTG AGCTGCTGGT TGTGTATGGA GCGGATCCGG GAGCTCCAGA TATCAATGGC 
CGAACACCCA TAGACTATGC ACGGCAAGCG GGTCACGTGG AGTTGGCCGA ACGCTTGGTG 
GAGTGTCAGT ATGAGCTCAC GGACAGACTG GCCTTCTACC TGTGTGGACG TCGCCCAGAT 
CATAAGAATG GTCATTATAT CATCCCTCAG ATGGCAGACA GAGCTCGCCC TAAGTGCCCG 
ACGCAGAGCC TGGACCTCTC TGAACTGGCC AAGGCAGCCA AGAAGAAGCT CCAAGCGTTA 
AATAATCGTT TGTTCGAGGA GCTGGCTATG GACGTGTACG ATGAAGTGGA CCGTAGAGAG 
AATGATGCAG TGTGGCTGAC AACTCAGAAC CACAGTACGC TGGTGACCGA ACGGAGCGCT 
GTCCCATTCT TACCCGTCAA TCCTGAATAC TCAGCGACAC GCAACCAGGG GCGGCAGAAA 
TTAGCACGCT TCAATGCGCG GGAATTCGCC ACCCTCATTA TCGACATCCT CAGCGACGCC 
AAGCGACGGC AACATGGAAA AGGCCTGACG AGCCCCACAG ACCCGCTGGA TCTGAGTCAG 
GTGGACGATG ACCAGCACGA TTATGACAGC GTAGCGTCTG ATGAAGACAC AGACAGCGAG 
CTGACCGCAC AGAATAATAA CAACACCCAA CGCAACAACC GGGCCAAGAG TATGGACTCG 
TCAGATCTGT CAGATGGACC AATCACACTA CAGGAGTATC TGGAGGTGAA GAAAGCACTG 
GCTTCCTCTG AGGCTAAAGT TCAGCAGCTC ATGAAGGTCA ACAACAACCT GAGTGAGGAG 
CTGCGGAGGC TGCAGAAAGA GGTGCGAAAG GGCGCTCCAG CGGGTCCCAC CCCATACGGA 
GGAGCGCACC TGTCAGCCTC CACTGACGGA CGCTATAATC ATCCAAAAGC AGGAGAGAAG 
TACGGGAGTG GGACTGATAG CGACTATGAC AACTCTCAAA CATATGACGT CTCCATTGGT 
GTGGGCCGCA GCAGTGAGGA GGACAGCAGG GGGGATGTAG ACGACACCGA GGGCGATCCT 
GACCCCACAC TGCCCTGCAC AGAGGACGTC ATCCTTAAAA CTGAACAGGT CACCAAAAAC 
ATCCAGGAGC TCCTGAGAGC TGCTCAAGAG TTCAAGCACG ACAGTTTTGT GCCATGCTCA 
GAGAAAATCC ACTTAGCTGT GACAGAAATG GCCTCGCTGT TTCCCAAGAG ACCAGCGCTG 
GATGCAGTAC GTTCTTCCTT GAAGCTCCTG GCAGCAAGTG CGTCACGTTT GCAGGTGGAG 
TGTCGTAAAG CGGCGCCTTC GGACTCGTCG GCCAGTACAG TGGACTACCA GCTTCTTACC 
CAGCAGGTCA TCCAGTGCGC ATACGACATT GCCAAAGCCG CCAAGCAGCT GGTCACCATC 
ACCACCCGCG AAAAAAAACA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

git1 ( XM_009291756.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290031.1 Danio rerio G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X3, mRNA.

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CloneID	ODa45617
Clone ID Related Accession (Same CDS sequence)	XM_009291756.1 , XM_009291756.2
Accession Version	XM_009291756.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ARF GTPase-activating protein GIT1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291756.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGGA AAGTCCAGAG AAGCGAGGTG TGCGCCGACT GCAGTGCACC AGACCCGGGC 
TGGAGCAGTA TAAACCGAGG AGTCCTGATC TGCGATGAGT GCTGTTCTGT ACACCGGAGC 
CTGGGCCGTC ACATTTCCAT CGTCAAACAC TTGAGACACA GCGGCTGGCC TCCTTCCTTA 
CTGCAGATGG TGCAGACCCT GGCCAGTAAC GGCGCTAACT CGATCTGGGA ACACTCTCTG 
CTGGATCCAG CACAAGTTCA GAGTGGCCGC AGGAAACCAA ACCCACAAGA TAAAGTCCAC 
CCCACAAAGT CAGAGTTCAT TCGCGCCAAG TACCAGATGT TAGCCTTTGT CCATAAGTTG 
CCCTGTCGTG ATGACGACGG AGTCACTACT AAGGACCTTA GCAAGCAACT GCACTCAAGT 
GTGCGGACAG GAAGTCTGGA GACATGTTTA CGGCTGCTGT CACTGGGGGC ACAAGCCAAC 
TTCTTCCACC CGGAAAAGGG CACAACCCCT CTCCATGTGG CTGCCAAAGC GGGACAGGTT 
CTCCAGGCTG AGCTGCTGGT TGTGTATGGA GCGGATCCGG GAGCTCCAGA TATCAATGGC 
CGAACACCCA TAGACTATGC ACGGCAAGCG GGTCACGTGG AGTTGGCCGA ACGCTTGGTG 
GAGTGTCAGT ATGAGCTCAC GGACAGACTG GCCTTCTACC TGTGTGGACG TCGCCCAGAT 
CATAAGAATG GTCATTATAT CATCCCTCAG ATGGCAGACA GAGCTCGCCC TAAGTGCCCG 
ACGCAGAGCC TGGACCTCTC TGAACTGGCC AAGGCAGCCA AGAAGAAGCT CCAAGCGTTA 
AATAATCGTT TGTTCGAGGA GCTGGCTATG GACGTGTACG ATGAAGTGGA CCGTAGAGAG 
AATGATGCAG TGTGGCTGAC AACTCAGAAC CACAGTACGC TGGTGACCGA ACGGAGCGCT 
GTCCCATTCT TACCCGTCAA TCCTGAATAC TCAGCGACAC GCAACCAGGG GCGGCAGAAA 
TTAGCACGCT TCAATGCGCG GGAATTCGCC ACCCTCATTA TCGACATCCT CAGCGACGCC 
AAGCGACGGC AACATGGAAA AGGCCTGACG AGCCCCACAG ACCCGCTGGA TCTGAGTCAG 
GTGGACGATG ACCAGCACGA TTATGACAGC GTAGCGTCTG ATGAAGACAC AGACAGCGAG 
CTGACCGCAC AGAATAATAA CAACACCCAA CGCAACAACC GGGCCAAGAG TATGGACTCG 
TCAGATCTGT CAGATGGACC AATCACACTA CAGGAGTATC TGGAGGTGAA GAAAGCACTG 
GCTTCCTCTG AGGCTAAAGT TCAGCAGCTC ATGAAGGTCA ACAACAACCT GAGTGAGGAG 
CTGCGGAGGC TGCAGAAAGA GGTGCGAAAG GGCGCTCCAG CGGGTCCCAC CCCATACGGA 
GGAGCGCACC TGTCAGCCTC CACTGACGGA CGCTATAATC ATCCAAAAGC AGGAGAGAAG 
TACGGGAGTG GGACTGATAG CGACTATGAC AACTCTCAAA CATATGACGT CTCCATTGGT 
GTGGGCCGCA GCAGTGAGGA GGACAGCAGG GGGGATGTAG ACGACACCGA GGGCGATCCT 
GACCCCACAC TGCCCTGCAC AGAGGACGTC ATCCTTAAAA CTGAACAGGT CACCAAAAAC 
ATCCAGGAGC TCCTGAGAGC TGCTCAAGAG TTCAAGCACG ACAGTTTTGT GCCATGCTCA 
GAGAAAATCC ACTTAGCTGT GACAGAAATG GCCTCGCTGT TTCCCAAGAG ACCAGCGCTG 
GATGCAGTAC GTTCTTCCTT GAAGCTCCTG GCAGCAAGTG CGTCACGTTT GCAGGTGGAG 
TGTCGTAAAG CGGCGCCTTC GGACTCGTCG GCCAGTACAG TGGACTACCA GCTTCTTACC 
CAGCAGGTCA TCCAGTGCGC ATACGACATT GCCAAAGCCG CCAAGCAGCT GGTCACCATC 
ACCACCCGCG AAAAAAAACA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290031.1
CDS	478..2481
Translation	MSRKVQRSEVCADCSAPDPGWSSINRGVLICDECCSVHRSLGRHISIVKHLRHSGWPPSLLQMVQTLASNGANSIWEHSLLDPAQVQSGRRKPNPQDKVHPTKSEFIRAKYQMLAFVHKLPCRDDDGVTTKDLSKQLHSSVRTGSLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQVLQAELLVVYGADPGAPDINGRTPIDYARQAGHVELAERLVECQYELTDRLAFYLCGRRPDHKNGHYIIPQMADRARPKCPTQSLDLSELAKAAKKKLQALNNRLFEELAMDVYDEVDRRENDAVWLTTQNHSTLVTERSAVPFLPVNPEYSATRNQGRQKLARFNAREFATLIIDILSDAKRRQHGKGLTSPTDPLDLSQVDDDQHDYDSVASDEDTDSELTAQNNNNTQRNNRAKSMDSSDLSDGPITLQEYLEVKKALASSEAKVQQLMKVNNNLSEELRRLQKEVRKGAPAGPTPYGGAHLSASTDGRYNHPKAGEKYGSGTDSDYDNSQTYDVSIGVGRSSEEDSRGDVDDTEGDPDPTLPCTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAVTEMASLFPKRPALDAVRSSLKLLAASASRLQVECRKAAPSDSSASTVDYQLLTQQVIQCAYDIAKAAKQLVTITTREKKQ

Target ORF information:

RefSeq Version	XM_009291756.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio G protein-coupled receptor kinase interacting ArfGAP 1 (git1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291756.2

ORF Insert Sequence:

ATGTCCAGGA AAGTCCAGAG AAGCGAGGTG TGCGCCGACT GCAGTGCACC AGACCCGGGC 
TGGAGCAGTA TAAACCGAGG AGTCCTGATC TGCGATGAGT GCTGTTCTGT ACACCGGAGC 
CTGGGCCGTC ACATTTCCAT CGTCAAACAC TTGAGACACA GCGGCTGGCC TCCTTCCTTA 
CTGCAGATGG TGCAGACCCT GGCCAGTAAC GGCGCTAACT CGATCTGGGA ACACTCTCTG 
CTGGATCCAG CACAAGTTCA GAGTGGCCGC AGGAAACCAA ACCCACAAGA TAAAGTCCAC 
CCCACAAAGT CAGAGTTCAT TCGCGCCAAG TACCAGATGT TAGCCTTTGT CCATAAGTTG 
CCCTGTCGTG ATGACGACGG AGTCACTACT AAGGACCTTA GCAAGCAACT GCACTCAAGT 
GTGCGGACAG GAAGTCTGGA GACATGTTTA CGGCTGCTGT CACTGGGGGC ACAAGCCAAC 
TTCTTCCACC CGGAAAAGGG CACAACCCCT CTCCATGTGG CTGCCAAAGC GGGACAGGTT 
CTCCAGGCTG AGCTGCTGGT TGTGTATGGA GCGGATCCGG GAGCTCCAGA TATCAATGGC 
CGAACACCCA TAGACTATGC ACGGCAAGCG GGTCACGTGG AGTTGGCCGA ACGCTTGGTG 
GAGTGTCAGT ATGAGCTCAC GGACAGACTG GCCTTCTACC TGTGTGGACG TCGCCCAGAT 
CATAAGAATG GTCATTATAT CATCCCTCAG ATGGCAGACA GAGCTCGCCC TAAGTGCCCG 
ACGCAGAGCC TGGACCTCTC TGAACTGGCC AAGGCAGCCA AGAAGAAGCT CCAAGCGTTA 
AATAATCGTT TGTTCGAGGA GCTGGCTATG GACGTGTACG ATGAAGTGGA CCGTAGAGAG 
AATGATGCAG TGTGGCTGAC AACTCAGAAC CACAGTACGC TGGTGACCGA ACGGAGCGCT 
GTCCCATTCT TACCCGTCAA TCCTGAATAC TCAGCGACAC GCAACCAGGG GCGGCAGAAA 
TTAGCACGCT TCAATGCGCG GGAATTCGCC ACCCTCATTA TCGACATCCT CAGCGACGCC 
AAGCGACGGC AACATGGAAA AGGCCTGACG AGCCCCACAG ACCCGCTGGA TCTGAGTCAG 
GTGGACGATG ACCAGCACGA TTATGACAGC GTAGCGTCTG ATGAAGACAC AGACAGCGAG 
CTGACCGCAC AGAATAATAA CAACACCCAA CGCAACAACC GGGCCAAGAG TATGGACTCG 
TCAGATCTGT CAGATGGACC AATCACACTA CAGGAGTATC TGGAGGTGAA GAAAGCACTG 
GCTTCCTCTG AGGCTAAAGT TCAGCAGCTC ATGAAGGTCA ACAACAACCT GAGTGAGGAG 
CTGCGGAGGC TGCAGAAAGA GGTGCGAAAG GGCGCTCCAG CGGGTCCCAC CCCATACGGA 
GGAGCGCACC TGTCAGCCTC CACTGACGGA CGCTATAATC ATCCAAAAGC AGGAGAGAAG 
TACGGGAGTG GGACTGATAG CGACTATGAC AACTCTCAAA CATATGACGT CTCCATTGGT 
GTGGGCCGCA GCAGTGAGGA GGACAGCAGG GGGGATGTAG ACGACACCGA GGGCGATCCT 
GACCCCACAC TGCCCTGCAC AGAGGACGTC ATCCTTAAAA CTGAACAGGT CACCAAAAAC 
ATCCAGGAGC TCCTGAGAGC TGCTCAAGAG TTCAAGCACG ACAGTTTTGT GCCATGCTCA 
GAGAAAATCC ACTTAGCTGT GACAGAAATG GCCTCGCTGT TTCCCAAGAG ACCAGCGCTG 
GATGCAGTAC GTTCTTCCTT GAAGCTCCTG GCAGCAAGTG CGTCACGTTT GCAGGTGGAG 
TGTCGTAAAG CGGCGCCTTC GGACTCGTCG GCCAGTACAG TGGACTACCA GCTTCTTACC 
CAGCAGGTCA TCCAGTGCGC ATACGACATT GCCAAAGCCG CCAAGCAGCT GGTCACCATC 
ACCACCCGCG AAAAAAAACA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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git1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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