kcnma1a cDNA ORF clone, Danio rerio(zebrafish)

The following kcnma1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the kcnma1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa45453 XM_009307251.3
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X34, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa45453 XM_017358786.1
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa45454 XM_017358787.1
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa45455 XM_017358788.1
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X30, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa45456 XM_017358789.1
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X31, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa45457 XM_017358790.1
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X32, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa148543 XM_021480644.1
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X35, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
ODa54732 XM_017358791.1
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X33, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
ODa148536 XM_021480633.1
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa148537 XM_021480635.1
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa148538 XM_021480637.1
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa148539 XM_021480639.1
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa148540 XM_021480640.1
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa148541 XM_021480641.1
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X27, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa148542 XM_021480642.1
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X33, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa54726 XM_017358780.2
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa54727 XM_017358781.2
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa54728 XM_017358782.2
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X29, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa54729 XM_017358783.2
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X30, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa54730 XM_017358784.2
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X31, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa54731 XM_017358785.2
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X32, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa31868 NM_001145600.1
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa18608 XM_005156655.4
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa18611 XM_005156658.4
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa18612 XM_005156659.4
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa18615 XM_005156662.4
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa18616 XM_005156663.4
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa18617 XM_005156664.4
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa18618 XM_005156665.4
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa18620 XM_005156667.4
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa18621 XM_005156668.4
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa18622 XM_005156669.4
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa18623 XM_005156670.4
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa18624 XM_005156671.4
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa45445 XM_009307242.3
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa45446 XM_009307243.3
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa45447 XM_009307244.3
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa45448 XM_009307245.3
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa45449 XM_009307246.3
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa45450 XM_009307247.3
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa45451 XM_009307248.3
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa45452 XM_009307250.3
Latest version!
Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
ODa45453 XM_009307251.2 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X28, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa45450 XM_009307247.2 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa18615 XM_005156662.3 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa54729 XM_017358783.1 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X24, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa45447 XM_009307244.2 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa18620 XM_005156667.3 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X17, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa54731 XM_017358785.1 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X26, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa45445 XM_009307242.2 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa45448 XM_009307245.2 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa45451 XM_009307248.2 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa18623 XM_005156670.3 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X20, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa54727 XM_017358781.1 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X22, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa18608 XM_005156655.3 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa18612 XM_005156659.3 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa18616 XM_005156663.3 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa18617 XM_005156664.3 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa18622 XM_005156669.3 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X19, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa18624 XM_005156671.3 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X21, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa54728 XM_017358782.1 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X23, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa45446 XM_009307243.2 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa18611 XM_005156658.3 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa45449 XM_009307246.2 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa18618 XM_005156665.3 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa54726 XM_017358780.1 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa45452 XM_009307250.2 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa18621 XM_005156668.3 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X18, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa54730 XM_017358784.1 Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X25, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa45453
    Clone ID Related Accession (Same CDS sequence) XM_017358786.1 , XM_009307251.2 , XM_009307251.3
    Accession Version XM_017358786.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2871bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X27
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTTTGCTC GCTACGTGCC AGAAATTGCT GCTCTCATCC TTAATCGGAA GAAATACGGA 
    GGGAGTTATA ACTCGACACG AGGCAGAAAG CATATTGTGG TCTGTGGTCA TATCACGCTC
    GAAAGTGTTT CCAACTTCCT TAAAGACTTC CTACACAAGG ACAGGGATGA TGTCAATGTA
    GAAATTGTTT TTCTTCATAA TATTTCCCCT AATCTTGAGC TGGAAGCCTT GTTCAAACGT
    CACTTCACTC AGGTGGAATT CTATCAAGGA TCGGTCCTCA ACCCACATGA TCTAGCAAGA
    GTGAAGATTG AGTCGGCGGA CGCTTGCTTG ATTCTTGCAA ACAAATACTG TGCAGACCCC
    GATGCTGAGG ATGCCTCAAA TATCATGAGA GTGATATCTA TCAAGAACTA CCATCCAAAG
    ATCAGAATAA TAACACAAAT GTTGCAGTAC CACAACAAGG CCCATCTGCT AAACATTCCC
    AGCTGGAACT GGAAGGAGGG AGATGATGCG ATTTGCCTGG CCGAGTTGAA GGCAGGCTTC
    ATAGCCCAGA GCTGCCTGGC ACAGGGCCTG TCCACCATGC TCGCCAACCT CTTCTCCATG
    AGGTCATACA TCAAGATTGA AGAAGACACA TGGCAGAAGT ATTATCTGGA AGGAGTAGCC
    AATGAAATGT ACACTGAGTA CCTGTCTAGT GCCTTTGTCG GCCTTTCATT TCCTACAGTT
    TGTGAGCTGT GCTACGTGAA GCTCAAGTTG CTGTTGATCG CTATTGAGTA CAAGTCAGAG
    CAAAGGGAAA GCAGAAGCAG AAAGCGTATT CTGATCAACC CGGGAAATCA CGTTAAAATG
    CAGGAGGGGA CACTGGGATT CTTTATTGCC AGTGATGCCA AAGAAGTGAA AAGAGCATTT
    TTCTACTGCA AAGCATGTCA TGATGACATC ACAGATCCCA AACGGATTAA AAAGTGTGGC
    TGCAAGAGAA TGACATATTC CAAGATGTCC GTCTACAAGC GAATGAAACT GGCATGTTGT
    TTTGATTGCG GGCGCTCTGA GCGCGACTGC TCGTGCATGT CAGGCAGTGT ACATAGTAAC
    ATGGACAGCC TTCAGAGGGC CTTCCCACTT TCTTCTGTCT CTGTTCATGA TTGCTCAACC
    AGTTTACGTG CCTCCAAATA TAAGTTTAAC GGATATGTCA GATCACTCGA AGATGAGCAT
    CCGTCAACAC TGTCACCCAA AAAGAAGCAG CGCAACGGAG GCATGCGAAA TTCACCCAAC
    TGCTCGCCCA AAATGATGAG GCATGATCCT CTACTCATTC CAGGAAATGA GCAGATTGAG
    AGCATGGATG CTAATGTCAA ACGATACGAC TCTACTGGAA TGTTTCACTG GTGTCCATCC
    AAAGAAATCG AGAAGGTCAT TCTGACACGC AGCGAGGCCT CAATGACGGT TTTAAGTGGA
    CATGTCGTAG TCTGCATATT TGGAGATGTC ACGTCAGCTT TAGTGGGGCT TCGAAACTTA
    GTCATGCCTT TGAGAGCAAG CAACTTCCAT TACCATGAGC TAAAGCCCAT TGTGTTTGTG
    GGCTCGTTAG ATTATCTGCG AAGAGAGTGG GAAACCCTTC ACAACTTCCC CAAAGTCTTC
    ATCTTACCTG GTACGCCATT AAGTCGAGCT GATTTAAGGG CCGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AACAATATCG ACGATGCTTC ACTGCAGGAC
    AAGGAATGCA TCTTGGCATC TCTTAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGTTTCACA CCTCCAGGGA TGGATAGGTC ATCTCCTGAT
    AACAGTCCCG TGCACGGCTT AGTGCGGCAG GCATCCGTCA CCACCGGATC AAACATCCCC
    ATCATAACTG AACTGGCTAA GCCTGGCAAA CTACTGCCTT TGGTTTCATT CAGTCAGGAA
    AAAAACAGTG GAACCAACAT ACAAATGATA ACAGAACTGG TGAACGACTC CAACGTTCAG
    TTTTTGGACC AAGACGACGA TGACGACCCA GACACTGAGC TCTACCTGAC CCAGCCGTTC
    GCCTGTGGTA CCGCATTCGC CGTCAGCGTG TTGGACTCCC TGATGAGTGC GACATACTTC
    AATGATAATA TCCTCACCCT GATCCGGACG CTGGTCACGG GAGGCGCCAC GCCGGAGCTG
    GAGGCCCTGT TGGCTGAGGA GAACGCTCTG AGAGGTGGCT ACAGCACGCC GCAGACGCTG
    GCTAACAGGG ACAGGTGTCG GGTCGCCCAG CTGGCCCTCT ACGATGGGCC CTTCGCAGAC
    TTGGGGGACG GAGGTTGCTA TGGAGACTTG TTCTGTAAAG CATTAAAAAC CTACAACATG
    CTGTGCTTTG GAATCTATAG ATTGCGAGAT GCACATTTAG GTGCACCAAG CCAGTGCACA
    AAAAGATATG TCATCACAAA CCCGCCTTAT GAGTTTGAGA TGGTGCCCAC GGACCTGATC
    TTCTGTCTGA TGCAGTTCGA CCACAACGCG GGCCAGTCCC GAGCGAGCCT GTCCCACTCC
    TCCCATTCGT CCCATTCCTC CAGCAAGAAG AGCTCGTCTG TTCACTCCAT CCCAGCCACC
    AACCGGCAGA ACCGCAGCAG CAAAGCACGT GAAGCCCGTG ACAAACAGAA TGCAACAAGG
    ATGAATAGAA TGGGCCCAGA AAAGAGATGG TTTACAGATG AAGCAGAAAA CGCATACCCA
    AGGAACATTC AAATCAAGCC TATGAGCACT CACATGGCCA ACCAAGTCAA TCAGTACAAA
    TCCACTAGCA GTCTGATTCC ACCTATCCGA GAAGTCGAAG ACGAATGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214275.1
    CDS84..2954
    Translation

    Target ORF information:

    RefSeq Version XM_017358786.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358786.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTTTGCTC GCTACGTGCC AGAAATTGCT GCTCTCATCC TTAATCGGAA GAAATACGGA 
    GGGAGTTATA ACTCGACACG AGGCAGAAAG CATATTGTGG TCTGTGGTCA TATCACGCTC
    GAAAGTGTTT CCAACTTCCT TAAAGACTTC CTACACAAGG ACAGGGATGA TGTCAATGTA
    GAAATTGTTT TTCTTCATAA TATTTCCCCT AATCTTGAGC TGGAAGCCTT GTTCAAACGT
    CACTTCACTC AGGTGGAATT CTATCAAGGA TCGGTCCTCA ACCCACATGA TCTAGCAAGA
    GTGAAGATTG AGTCGGCGGA CGCTTGCTTG ATTCTTGCAA ACAAATACTG TGCAGACCCC
    GATGCTGAGG ATGCCTCAAA TATCATGAGA GTGATATCTA TCAAGAACTA CCATCCAAAG
    ATCAGAATAA TAACACAAAT GTTGCAGTAC CACAACAAGG CCCATCTGCT AAACATTCCC
    AGCTGGAACT GGAAGGAGGG AGATGATGCG ATTTGCCTGG CCGAGTTGAA GGCAGGCTTC
    ATAGCCCAGA GCTGCCTGGC ACAGGGCCTG TCCACCATGC TCGCCAACCT CTTCTCCATG
    AGGTCATACA TCAAGATTGA AGAAGACACA TGGCAGAAGT ATTATCTGGA AGGAGTAGCC
    AATGAAATGT ACACTGAGTA CCTGTCTAGT GCCTTTGTCG GCCTTTCATT TCCTACAGTT
    TGTGAGCTGT GCTACGTGAA GCTCAAGTTG CTGTTGATCG CTATTGAGTA CAAGTCAGAG
    CAAAGGGAAA GCAGAAGCAG AAAGCGTATT CTGATCAACC CGGGAAATCA CGTTAAAATG
    CAGGAGGGGA CACTGGGATT CTTTATTGCC AGTGATGCCA AAGAAGTGAA AAGAGCATTT
    TTCTACTGCA AAGCATGTCA TGATGACATC ACAGATCCCA AACGGATTAA AAAGTGTGGC
    TGCAAGAGAA TGACATATTC CAAGATGTCC GTCTACAAGC GAATGAAACT GGCATGTTGT
    TTTGATTGCG GGCGCTCTGA GCGCGACTGC TCGTGCATGT CAGGCAGTGT ACATAGTAAC
    ATGGACAGCC TTCAGAGGGC CTTCCCACTT TCTTCTGTCT CTGTTCATGA TTGCTCAACC
    AGTTTACGTG CCTCCAAATA TAAGTTTAAC GGATATGTCA GATCACTCGA AGATGAGCAT
    CCGTCAACAC TGTCACCCAA AAAGAAGCAG CGCAACGGAG GCATGCGAAA TTCACCCAAC
    TGCTCGCCCA AAATGATGAG GCATGATCCT CTACTCATTC CAGGAAATGA GCAGATTGAG
    AGCATGGATG CTAATGTCAA ACGATACGAC TCTACTGGAA TGTTTCACTG GTGTCCATCC
    AAAGAAATCG AGAAGGTCAT TCTGACACGC AGCGAGGCCT CAATGACGGT TTTAAGTGGA
    CATGTCGTAG TCTGCATATT TGGAGATGTC ACGTCAGCTT TAGTGGGGCT TCGAAACTTA
    GTCATGCCTT TGAGAGCAAG CAACTTCCAT TACCATGAGC TAAAGCCCAT TGTGTTTGTG
    GGCTCGTTAG ATTATCTGCG AAGAGAGTGG GAAACCCTTC ACAACTTCCC CAAAGTCTTC
    ATCTTACCTG GTACGCCATT AAGTCGAGCT GATTTAAGGG CCGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AACAATATCG ACGATGCTTC ACTGCAGGAC
    AAGGAATGCA TCTTGGCATC TCTTAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGTTTCACA CCTCCAGGGA TGGATAGGTC ATCTCCTGAT
    AACAGTCCCG TGCACGGCTT AGTGCGGCAG GCATCCGTCA CCACCGGATC AAACATCCCC
    ATCATAACTG AACTGGCTAA GCCTGGCAAA CTACTGCCTT TGGTTTCATT CAGTCAGGAA
    AAAAACAGTG GAACCAACAT ACAAATGATA ACAGAACTGG TGAACGACTC CAACGTTCAG
    TTTTTGGACC AAGACGACGA TGACGACCCA GACACTGAGC TCTACCTGAC CCAGCCGTTC
    GCCTGTGGTA CCGCATTCGC CGTCAGCGTG TTGGACTCCC TGATGAGTGC GACATACTTC
    AATGATAATA TCCTCACCCT GATCCGGACG CTGGTCACGG GAGGCGCCAC GCCGGAGCTG
    GAGGCCCTGT TGGCTGAGGA GAACGCTCTG AGAGGTGGCT ACAGCACGCC GCAGACGCTG
    GCTAACAGGG ACAGGTGTCG GGTCGCCCAG CTGGCCCTCT ACGATGGGCC CTTCGCAGAC
    TTGGGGGACG GAGGTTGCTA TGGAGACTTG TTCTGTAAAG CATTAAAAAC CTACAACATG
    CTGTGCTTTG GAATCTATAG ATTGCGAGAT GCACATTTAG GTGCACCAAG CCAGTGCACA
    AAAAGATATG TCATCACAAA CCCGCCTTAT GAGTTTGAGA TGGTGCCCAC GGACCTGATC
    TTCTGTCTGA TGCAGTTCGA CCACAACGCG GGCCAGTCCC GAGCGAGCCT GTCCCACTCC
    TCCCATTCGT CCCATTCCTC CAGCAAGAAG AGCTCGTCTG TTCACTCCAT CCCAGCCACC
    AACCGGCAGA ACCGCAGCAG CAAAGCACGT GAAGCCCGTG ACAAACAGAA TGCAACAAGG
    ATGAATAGAA TGGGCCCAGA AAAGAGATGG TTTACAGATG AAGCAGAAAA CGCATACCCA
    AGGAACATTC AAATCAAGCC TATGAGCACT CACATGGCCA ACCAAGTCAA TCAGTACAAA
    TCCACTAGCA GTCTGATTCC ACCTATCCGA GAAGTCGAAG ACGAATGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45453
    Clone ID Related Accession (Same CDS sequence) XM_017358786.1 , XM_009307251.2 , XM_009307251.3
    Accession Version XM_009307251.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2871bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X27
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307251.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTTTGCTC GCTACGTGCC AGAAATTGCT GCTCTCATCC TTAATCGGAA GAAATACGGA 
    GGGAGTTATA ACTCGACACG AGGCAGAAAG CATATTGTGG TCTGTGGTCA TATCACGCTC
    GAAAGTGTTT CCAACTTCCT TAAAGACTTC CTACACAAGG ACAGGGATGA TGTCAATGTA
    GAAATTGTTT TTCTTCATAA TATTTCCCCT AATCTTGAGC TGGAAGCCTT GTTCAAACGT
    CACTTCACTC AGGTGGAATT CTATCAAGGA TCGGTCCTCA ACCCACATGA TCTAGCAAGA
    GTGAAGATTG AGTCGGCGGA CGCTTGCTTG ATTCTTGCAA ACAAATACTG TGCAGACCCC
    GATGCTGAGG ATGCCTCAAA TATCATGAGA GTGATATCTA TCAAGAACTA CCATCCAAAG
    ATCAGAATAA TAACACAAAT GTTGCAGTAC CACAACAAGG CCCATCTGCT AAACATTCCC
    AGCTGGAACT GGAAGGAGGG AGATGATGCG ATTTGCCTGG CCGAGTTGAA GGCAGGCTTC
    ATAGCCCAGA GCTGCCTGGC ACAGGGCCTG TCCACCATGC TCGCCAACCT CTTCTCCATG
    AGGTCATACA TCAAGATTGA AGAAGACACA TGGCAGAAGT ATTATCTGGA AGGAGTAGCC
    AATGAAATGT ACACTGAGTA CCTGTCTAGT GCCTTTGTCG GCCTTTCATT TCCTACAGTT
    TGTGAGCTGT GCTACGTGAA GCTCAAGTTG CTGTTGATCG CTATTGAGTA CAAGTCAGAG
    CAAAGGGAAA GCAGAAGCAG AAAGCGTATT CTGATCAACC CGGGAAATCA CGTTAAAATG
    CAGGAGGGGA CACTGGGATT CTTTATTGCC AGTGATGCCA AAGAAGTGAA AAGAGCATTT
    TTCTACTGCA AAGCATGTCA TGATGACATC ACAGATCCCA AACGGATTAA AAAGTGTGGC
    TGCAAGAGAA TGACATATTC CAAGATGTCC GTCTACAAGC GAATGAAACT GGCATGTTGT
    TTTGATTGCG GGCGCTCTGA GCGCGACTGC TCGTGCATGT CAGGCAGTGT ACATAGTAAC
    ATGGACAGCC TTCAGAGGGC CTTCCCACTT TCTTCTGTCT CTGTTCATGA TTGCTCAACC
    AGTTTACGTG CCTCCAAATA TAAGTTTAAC GGATATGTCA GATCACTCGA AGATGAGCAT
    CCGTCAACAC TGTCACCCAA AAAGAAGCAG CGCAACGGAG GCATGCGAAA TTCACCCAAC
    TGCTCGCCCA AAATGATGAG GCATGATCCT CTACTCATTC CAGGAAATGA GCAGATTGAG
    AGCATGGATG CTAATGTCAA ACGATACGAC TCTACTGGAA TGTTTCACTG GTGTCCATCC
    AAAGAAATCG AGAAGGTCAT TCTGACACGC AGCGAGGCCT CAATGACGGT TTTAAGTGGA
    CATGTCGTAG TCTGCATATT TGGAGATGTC ACGTCAGCTT TAGTGGGGCT TCGAAACTTA
    GTCATGCCTT TGAGAGCAAG CAACTTCCAT TACCATGAGC TAAAGCCCAT TGTGTTTGTG
    GGCTCGTTAG ATTATCTGCG AAGAGAGTGG GAAACCCTTC ACAACTTCCC CAAAGTCTTC
    ATCTTACCTG GTACGCCATT AAGTCGAGCT GATTTAAGGG CCGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AACAATATCG ACGATGCTTC ACTGCAGGAC
    AAGGAATGCA TCTTGGCATC TCTTAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGTTTCACA CCTCCAGGGA TGGATAGGTC ATCTCCTGAT
    AACAGTCCCG TGCACGGCTT AGTGCGGCAG GCATCCGTCA CCACCGGATC AAACATCCCC
    ATCATAACTG AACTGGCTAA GCCTGGCAAA CTACTGCCTT TGGTTTCATT CAGTCAGGAA
    AAAAACAGTG GAACCAACAT ACAAATGATA ACAGAACTGG TGAACGACTC CAACGTTCAG
    TTTTTGGACC AAGACGACGA TGACGACCCA GACACTGAGC TCTACCTGAC CCAGCCGTTC
    GCCTGTGGTA CCGCATTCGC CGTCAGCGTG TTGGACTCCC TGATGAGTGC GACATACTTC
    AATGATAATA TCCTCACCCT GATCCGGACG CTGGTCACGG GAGGCGCCAC GCCGGAGCTG
    GAGGCCCTGT TGGCTGAGGA GAACGCTCTG AGAGGTGGCT ACAGCACGCC GCAGACGCTG
    GCTAACAGGG ACAGGTGTCG GGTCGCCCAG CTGGCCCTCT ACGATGGGCC CTTCGCAGAC
    TTGGGGGACG GAGGTTGCTA TGGAGACTTG TTCTGTAAAG CATTAAAAAC CTACAACATG
    CTGTGCTTTG GAATCTATAG ATTGCGAGAT GCACATTTAG GTGCACCAAG CCAGTGCACA
    AAAAGATATG TCATCACAAA CCCGCCTTAT GAGTTTGAGA TGGTGCCCAC GGACCTGATC
    TTCTGTCTGA TGCAGTTCGA CCACAACGCG GGCCAGTCCC GAGCGAGCCT GTCCCACTCC
    TCCCATTCGT CCCATTCCTC CAGCAAGAAG AGCTCGTCTG TTCACTCCAT CCCAGCCACC
    AACCGGCAGA ACCGCAGCAG CAAAGCACGT GAAGCCCGTG ACAAACAGAA TGCAACAAGG
    ATGAATAGAA TGGGCCCAGA AAAGAGATGG TTTACAGATG AAGCAGAAAA CGCATACCCA
    AGGAACATTC AAATCAAGCC TATGAGCACT CACATGGCCA ACCAAGTCAA TCAGTACAAA
    TCCACTAGCA GTCTGATTCC ACCTATCCGA GAAGTCGAAG ACGAATGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305526.1
    CDS91..2961
    Translation

    Target ORF information:

    RefSeq Version XM_009307251.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307251.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTTTGCTC GCTACGTGCC AGAAATTGCT GCTCTCATCC TTAATCGGAA GAAATACGGA 
    GGGAGTTATA ACTCGACACG AGGCAGAAAG CATATTGTGG TCTGTGGTCA TATCACGCTC
    GAAAGTGTTT CCAACTTCCT TAAAGACTTC CTACACAAGG ACAGGGATGA TGTCAATGTA
    GAAATTGTTT TTCTTCATAA TATTTCCCCT AATCTTGAGC TGGAAGCCTT GTTCAAACGT
    CACTTCACTC AGGTGGAATT CTATCAAGGA TCGGTCCTCA ACCCACATGA TCTAGCAAGA
    GTGAAGATTG AGTCGGCGGA CGCTTGCTTG ATTCTTGCAA ACAAATACTG TGCAGACCCC
    GATGCTGAGG ATGCCTCAAA TATCATGAGA GTGATATCTA TCAAGAACTA CCATCCAAAG
    ATCAGAATAA TAACACAAAT GTTGCAGTAC CACAACAAGG CCCATCTGCT AAACATTCCC
    AGCTGGAACT GGAAGGAGGG AGATGATGCG ATTTGCCTGG CCGAGTTGAA GGCAGGCTTC
    ATAGCCCAGA GCTGCCTGGC ACAGGGCCTG TCCACCATGC TCGCCAACCT CTTCTCCATG
    AGGTCATACA TCAAGATTGA AGAAGACACA TGGCAGAAGT ATTATCTGGA AGGAGTAGCC
    AATGAAATGT ACACTGAGTA CCTGTCTAGT GCCTTTGTCG GCCTTTCATT TCCTACAGTT
    TGTGAGCTGT GCTACGTGAA GCTCAAGTTG CTGTTGATCG CTATTGAGTA CAAGTCAGAG
    CAAAGGGAAA GCAGAAGCAG AAAGCGTATT CTGATCAACC CGGGAAATCA CGTTAAAATG
    CAGGAGGGGA CACTGGGATT CTTTATTGCC AGTGATGCCA AAGAAGTGAA AAGAGCATTT
    TTCTACTGCA AAGCATGTCA TGATGACATC ACAGATCCCA AACGGATTAA AAAGTGTGGC
    TGCAAGAGAA TGACATATTC CAAGATGTCC GTCTACAAGC GAATGAAACT GGCATGTTGT
    TTTGATTGCG GGCGCTCTGA GCGCGACTGC TCGTGCATGT CAGGCAGTGT ACATAGTAAC
    ATGGACAGCC TTCAGAGGGC CTTCCCACTT TCTTCTGTCT CTGTTCATGA TTGCTCAACC
    AGTTTACGTG CCTCCAAATA TAAGTTTAAC GGATATGTCA GATCACTCGA AGATGAGCAT
    CCGTCAACAC TGTCACCCAA AAAGAAGCAG CGCAACGGAG GCATGCGAAA TTCACCCAAC
    TGCTCGCCCA AAATGATGAG GCATGATCCT CTACTCATTC CAGGAAATGA GCAGATTGAG
    AGCATGGATG CTAATGTCAA ACGATACGAC TCTACTGGAA TGTTTCACTG GTGTCCATCC
    AAAGAAATCG AGAAGGTCAT TCTGACACGC AGCGAGGCCT CAATGACGGT TTTAAGTGGA
    CATGTCGTAG TCTGCATATT TGGAGATGTC ACGTCAGCTT TAGTGGGGCT TCGAAACTTA
    GTCATGCCTT TGAGAGCAAG CAACTTCCAT TACCATGAGC TAAAGCCCAT TGTGTTTGTG
    GGCTCGTTAG ATTATCTGCG AAGAGAGTGG GAAACCCTTC ACAACTTCCC CAAAGTCTTC
    ATCTTACCTG GTACGCCATT AAGTCGAGCT GATTTAAGGG CCGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AACAATATCG ACGATGCTTC ACTGCAGGAC
    AAGGAATGCA TCTTGGCATC TCTTAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGTTTCACA CCTCCAGGGA TGGATAGGTC ATCTCCTGAT
    AACAGTCCCG TGCACGGCTT AGTGCGGCAG GCATCCGTCA CCACCGGATC AAACATCCCC
    ATCATAACTG AACTGGCTAA GCCTGGCAAA CTACTGCCTT TGGTTTCATT CAGTCAGGAA
    AAAAACAGTG GAACCAACAT ACAAATGATA ACAGAACTGG TGAACGACTC CAACGTTCAG
    TTTTTGGACC AAGACGACGA TGACGACCCA GACACTGAGC TCTACCTGAC CCAGCCGTTC
    GCCTGTGGTA CCGCATTCGC CGTCAGCGTG TTGGACTCCC TGATGAGTGC GACATACTTC
    AATGATAATA TCCTCACCCT GATCCGGACG CTGGTCACGG GAGGCGCCAC GCCGGAGCTG
    GAGGCCCTGT TGGCTGAGGA GAACGCTCTG AGAGGTGGCT ACAGCACGCC GCAGACGCTG
    GCTAACAGGG ACAGGTGTCG GGTCGCCCAG CTGGCCCTCT ACGATGGGCC CTTCGCAGAC
    TTGGGGGACG GAGGTTGCTA TGGAGACTTG TTCTGTAAAG CATTAAAAAC CTACAACATG
    CTGTGCTTTG GAATCTATAG ATTGCGAGAT GCACATTTAG GTGCACCAAG CCAGTGCACA
    AAAAGATATG TCATCACAAA CCCGCCTTAT GAGTTTGAGA TGGTGCCCAC GGACCTGATC
    TTCTGTCTGA TGCAGTTCGA CCACAACGCG GGCCAGTCCC GAGCGAGCCT GTCCCACTCC
    TCCCATTCGT CCCATTCCTC CAGCAAGAAG AGCTCGTCTG TTCACTCCAT CCCAGCCACC
    AACCGGCAGA ACCGCAGCAG CAAAGCACGT GAAGCCCGTG ACAAACAGAA TGCAACAAGG
    ATGAATAGAA TGGGCCCAGA AAAGAGATGG TTTACAGATG AAGCAGAAAA CGCATACCCA
    AGGAACATTC AAATCAAGCC TATGAGCACT CACATGGCCA ACCAAGTCAA TCAGTACAAA
    TCCACTAGCA GTCTGATTCC ACCTATCCGA GAAGTCGAAG ACGAATGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45453
    Clone ID Related Accession (Same CDS sequence) XM_017358786.1 , XM_009307251.2 , XM_009307251.3
    Accession Version XM_009307251.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2871bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X34
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307251.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTTTGCTC GCTACGTGCC AGAAATTGCT GCTCTCATCC TTAATCGGAA GAAATACGGA 
    GGGAGTTATA ACTCGACACG AGGCAGAAAG CATATTGTGG TCTGTGGTCA TATCACGCTC
    GAAAGTGTTT CCAACTTCCT TAAAGACTTC CTACACAAGG ACAGGGATGA TGTCAATGTA
    GAAATTGTTT TTCTTCATAA TATTTCCCCT AATCTTGAGC TGGAAGCCTT GTTCAAACGT
    CACTTCACTC AGGTGGAATT CTATCAAGGA TCGGTCCTCA ACCCACATGA TCTAGCAAGA
    GTGAAGATTG AGTCGGCGGA CGCTTGCTTG ATTCTTGCAA ACAAATACTG TGCAGACCCC
    GATGCTGAGG ATGCCTCAAA TATCATGAGA GTGATATCTA TCAAGAACTA CCATCCAAAG
    ATCAGAATAA TAACACAAAT GTTGCAGTAC CACAACAAGG CCCATCTGCT AAACATTCCC
    AGCTGGAACT GGAAGGAGGG AGATGATGCG ATTTGCCTGG CCGAGTTGAA GGCAGGCTTC
    ATAGCCCAGA GCTGCCTGGC ACAGGGCCTG TCCACCATGC TCGCCAACCT CTTCTCCATG
    AGGTCATACA TCAAGATTGA AGAAGACACA TGGCAGAAGT ATTATCTGGA AGGAGTAGCC
    AATGAAATGT ACACTGAGTA CCTGTCTAGT GCCTTTGTCG GCCTTTCATT TCCTACAGTT
    TGTGAGCTGT GCTACGTGAA GCTCAAGTTG CTGTTGATCG CTATTGAGTA CAAGTCAGAG
    CAAAGGGAAA GCAGAAGCAG AAAGCGTATT CTGATCAACC CGGGAAATCA CGTTAAAATG
    CAGGAGGGGA CACTGGGATT CTTTATTGCC AGTGATGCCA AAGAAGTGAA AAGAGCATTT
    TTCTACTGCA AAGCATGTCA TGATGACATC ACAGATCCCA AACGGATTAA AAAGTGTGGC
    TGCAAGAGAA TGACATATTC CAAGATGTCC GTCTACAAGC GAATGAAACT GGCATGTTGT
    TTTGATTGCG GGCGCTCTGA GCGCGACTGC TCGTGCATGT CAGGCAGTGT ACATAGTAAC
    ATGGACAGCC TTCAGAGGGC CTTCCCACTT TCTTCTGTCT CTGTTCATGA TTGCTCAACC
    AGTTTACGTG CCTCCAAATA TAAGTTTAAC GGATATGTCA GATCACTCGA AGATGAGCAT
    CCGTCAACAC TGTCACCCAA AAAGAAGCAG CGCAACGGAG GCATGCGAAA TTCACCCAAC
    TGCTCGCCCA AAATGATGAG GCATGATCCT CTACTCATTC CAGGAAATGA GCAGATTGAG
    AGCATGGATG CTAATGTCAA ACGATACGAC TCTACTGGAA TGTTTCACTG GTGTCCATCC
    AAAGAAATCG AGAAGGTCAT TCTGACACGC AGCGAGGCCT CAATGACGGT TTTAAGTGGA
    CATGTCGTAG TCTGCATATT TGGAGATGTC ACGTCAGCTT TAGTGGGGCT TCGAAACTTA
    GTCATGCCTT TGAGAGCAAG CAACTTCCAT TACCATGAGC TAAAGCCCAT TGTGTTTGTG
    GGCTCGTTAG ATTATCTGCG AAGAGAGTGG GAAACCCTTC ACAACTTCCC CAAAGTCTTC
    ATCTTACCTG GTACGCCATT AAGTCGAGCT GATTTAAGGG CCGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AACAATATCG ACGATGCTTC ACTGCAGGAC
    AAGGAATGCA TCTTGGCATC TCTTAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGTTTCACA CCTCCAGGGA TGGATAGGTC ATCTCCTGAT
    AACAGTCCCG TGCACGGCTT AGTGCGGCAG GCATCCGTCA CCACCGGATC AAACATCCCC
    ATCATAACTG AACTGGCTAA GCCTGGCAAA CTACTGCCTT TGGTTTCATT CAGTCAGGAA
    AAAAACAGTG GAACCAACAT ACAAATGATA ACAGAACTGG TGAACGACTC CAACGTTCAG
    TTTTTGGACC AAGACGACGA TGACGACCCA GACACTGAGC TCTACCTGAC CCAGCCGTTC
    GCCTGTGGTA CCGCATTCGC CGTCAGCGTG TTGGACTCCC TGATGAGTGC GACATACTTC
    AATGATAATA TCCTCACCCT GATCCGGACG CTGGTCACGG GAGGCGCCAC GCCGGAGCTG
    GAGGCCCTGT TGGCTGAGGA GAACGCTCTG AGAGGTGGCT ACAGCACGCC GCAGACGCTG
    GCTAACAGGG ACAGGTGTCG GGTCGCCCAG CTGGCCCTCT ACGATGGGCC CTTCGCAGAC
    TTGGGGGACG GAGGTTGCTA TGGAGACTTG TTCTGTAAAG CATTAAAAAC CTACAACATG
    CTGTGCTTTG GAATCTATAG ATTGCGAGAT GCACATTTAG GTGCACCAAG CCAGTGCACA
    AAAAGATATG TCATCACAAA CCCGCCTTAT GAGTTTGAGA TGGTGCCCAC GGACCTGATC
    TTCTGTCTGA TGCAGTTCGA CCACAACGCG GGCCAGTCCC GAGCGAGCCT GTCCCACTCC
    TCCCATTCGT CCCATTCCTC CAGCAAGAAG AGCTCGTCTG TTCACTCCAT CCCAGCCACC
    AACCGGCAGA ACCGCAGCAG CAAAGCACGT GAAGCCCGTG ACAAACAGAA TGCAACAAGG
    ATGAATAGAA TGGGCCCAGA AAAGAGATGG TTTACAGATG AAGCAGAAAA CGCATACCCA
    AGGAACATTC AAATCAAGCC TATGAGCACT CACATGGCCA ACCAAGTCAA TCAGTACAAA
    TCCACTAGCA GTCTGATTCC ACCTATCCGA GAAGTCGAAG ACGAATGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305526.1
    CDS91..2961
    Translation

    Target ORF information:

    RefSeq Version XM_009307251.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X34, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307251.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTTTGCTC GCTACGTGCC AGAAATTGCT GCTCTCATCC TTAATCGGAA GAAATACGGA 
    GGGAGTTATA ACTCGACACG AGGCAGAAAG CATATTGTGG TCTGTGGTCA TATCACGCTC
    GAAAGTGTTT CCAACTTCCT TAAAGACTTC CTACACAAGG ACAGGGATGA TGTCAATGTA
    GAAATTGTTT TTCTTCATAA TATTTCCCCT AATCTTGAGC TGGAAGCCTT GTTCAAACGT
    CACTTCACTC AGGTGGAATT CTATCAAGGA TCGGTCCTCA ACCCACATGA TCTAGCAAGA
    GTGAAGATTG AGTCGGCGGA CGCTTGCTTG ATTCTTGCAA ACAAATACTG TGCAGACCCC
    GATGCTGAGG ATGCCTCAAA TATCATGAGA GTGATATCTA TCAAGAACTA CCATCCAAAG
    ATCAGAATAA TAACACAAAT GTTGCAGTAC CACAACAAGG CCCATCTGCT AAACATTCCC
    AGCTGGAACT GGAAGGAGGG AGATGATGCG ATTTGCCTGG CCGAGTTGAA GGCAGGCTTC
    ATAGCCCAGA GCTGCCTGGC ACAGGGCCTG TCCACCATGC TCGCCAACCT CTTCTCCATG
    AGGTCATACA TCAAGATTGA AGAAGACACA TGGCAGAAGT ATTATCTGGA AGGAGTAGCC
    AATGAAATGT ACACTGAGTA CCTGTCTAGT GCCTTTGTCG GCCTTTCATT TCCTACAGTT
    TGTGAGCTGT GCTACGTGAA GCTCAAGTTG CTGTTGATCG CTATTGAGTA CAAGTCAGAG
    CAAAGGGAAA GCAGAAGCAG AAAGCGTATT CTGATCAACC CGGGAAATCA CGTTAAAATG
    CAGGAGGGGA CACTGGGATT CTTTATTGCC AGTGATGCCA AAGAAGTGAA AAGAGCATTT
    TTCTACTGCA AAGCATGTCA TGATGACATC ACAGATCCCA AACGGATTAA AAAGTGTGGC
    TGCAAGAGAA TGACATATTC CAAGATGTCC GTCTACAAGC GAATGAAACT GGCATGTTGT
    TTTGATTGCG GGCGCTCTGA GCGCGACTGC TCGTGCATGT CAGGCAGTGT ACATAGTAAC
    ATGGACAGCC TTCAGAGGGC CTTCCCACTT TCTTCTGTCT CTGTTCATGA TTGCTCAACC
    AGTTTACGTG CCTCCAAATA TAAGTTTAAC GGATATGTCA GATCACTCGA AGATGAGCAT
    CCGTCAACAC TGTCACCCAA AAAGAAGCAG CGCAACGGAG GCATGCGAAA TTCACCCAAC
    TGCTCGCCCA AAATGATGAG GCATGATCCT CTACTCATTC CAGGAAATGA GCAGATTGAG
    AGCATGGATG CTAATGTCAA ACGATACGAC TCTACTGGAA TGTTTCACTG GTGTCCATCC
    AAAGAAATCG AGAAGGTCAT TCTGACACGC AGCGAGGCCT CAATGACGGT TTTAAGTGGA
    CATGTCGTAG TCTGCATATT TGGAGATGTC ACGTCAGCTT TAGTGGGGCT TCGAAACTTA
    GTCATGCCTT TGAGAGCAAG CAACTTCCAT TACCATGAGC TAAAGCCCAT TGTGTTTGTG
    GGCTCGTTAG ATTATCTGCG AAGAGAGTGG GAAACCCTTC ACAACTTCCC CAAAGTCTTC
    ATCTTACCTG GTACGCCATT AAGTCGAGCT GATTTAAGGG CCGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AACAATATCG ACGATGCTTC ACTGCAGGAC
    AAGGAATGCA TCTTGGCATC TCTTAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGTTTCACA CCTCCAGGGA TGGATAGGTC ATCTCCTGAT
    AACAGTCCCG TGCACGGCTT AGTGCGGCAG GCATCCGTCA CCACCGGATC AAACATCCCC
    ATCATAACTG AACTGGCTAA GCCTGGCAAA CTACTGCCTT TGGTTTCATT CAGTCAGGAA
    AAAAACAGTG GAACCAACAT ACAAATGATA ACAGAACTGG TGAACGACTC CAACGTTCAG
    TTTTTGGACC AAGACGACGA TGACGACCCA GACACTGAGC TCTACCTGAC CCAGCCGTTC
    GCCTGTGGTA CCGCATTCGC CGTCAGCGTG TTGGACTCCC TGATGAGTGC GACATACTTC
    AATGATAATA TCCTCACCCT GATCCGGACG CTGGTCACGG GAGGCGCCAC GCCGGAGCTG
    GAGGCCCTGT TGGCTGAGGA GAACGCTCTG AGAGGTGGCT ACAGCACGCC GCAGACGCTG
    GCTAACAGGG ACAGGTGTCG GGTCGCCCAG CTGGCCCTCT ACGATGGGCC CTTCGCAGAC
    TTGGGGGACG GAGGTTGCTA TGGAGACTTG TTCTGTAAAG CATTAAAAAC CTACAACATG
    CTGTGCTTTG GAATCTATAG ATTGCGAGAT GCACATTTAG GTGCACCAAG CCAGTGCACA
    AAAAGATATG TCATCACAAA CCCGCCTTAT GAGTTTGAGA TGGTGCCCAC GGACCTGATC
    TTCTGTCTGA TGCAGTTCGA CCACAACGCG GGCCAGTCCC GAGCGAGCCT GTCCCACTCC
    TCCCATTCGT CCCATTCCTC CAGCAAGAAG AGCTCGTCTG TTCACTCCAT CCCAGCCACC
    AACCGGCAGA ACCGCAGCAG CAAAGCACGT GAAGCCCGTG ACAAACAGAA TGCAACAAGG
    ATGAATAGAA TGGGCCCAGA AAAGAGATGG TTTACAGATG AAGCAGAAAA CGCATACCCA
    AGGAACATTC AAATCAAGCC TATGAGCACT CACATGGCCA ACCAAGTCAA TCAGTACAAA
    TCCACTAGCA GTCTGATTCC ACCTATCCGA GAAGTCGAAG ACGAATGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45454
    Clone ID Related Accession (Same CDS sequence) XM_017358787.1
    Accession Version XM_017358787.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2580bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X28
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTTTGCTC GCTACGTGCC AGAAATTGCT GCTCTCATCC TTAATCGGAA GAAATACGGA 
    GGGAGTTATA ACTCGACACG AGGCAGAAAG CATATTGTGG TCTGTGGTCA TATCACGCTC
    GAAAGTGTTT CCAACTTCCT TAAAGACTTC CTACACAAGG ACAGGGATGA TGTCAATGTA
    GAAATTGTTT TTCTTCATAA TATTTCCCCT AATCTTGAGC TGGAAGCCTT GTTCAAACGT
    CACTTCACTC AGGTGGAATT CTATCAAGGA TCGGTCCTCA ACCCACATGA TCTAGCAAGA
    GTGAAGATTG AGTCGGCGGA CGCTTGCTTG ATTCTTGCAA ACAAATACTG TGCAGACCCC
    GATGCTGAGG ATGCCTCAAA TATCATGAGA GTGATATCTA TCAAGAACTA CCATCCAAAG
    ATCAGAATAA TAACACAAAT GTTGCAGTAC CACAACAAGG CCCATCTGCT AAACATTCCC
    AGCTGGAACT GGAAGGAGGG AGATGATGCG ATTTGCCTGG CCGAGTTGAA GGCAGGCTTC
    ATAGCCCAGA GCTGCCTGGC ACAGGGCCTG TCCACCATGC TCGCCAACCT CTTCTCCATG
    AGGTCATACA TCAAGATTGA AGAAGACACA TGGCAGAAGT ATTATCTGGA AGGAGTAGCC
    AATGAAATGT ACACTGAGTA CCTGTCTAGT GCCTTTGTCG GCCTTTCATT TCCTACAGTT
    TGTGAGCTGT GCTACGTGAA GCTCAAGTTG CTGTTGATCG CTATTGAGTA CAAGTCAGAG
    CAAAGGGAAA GCAGAAGCAG AAAGCGTATT CTGATCAACC CGGGAAATCA CGTTAAAATG
    CAGGAGGGGA CACTGGGATT CTTTATTGCC AGTGATGCCA AAGAAGTGAA AAGAGCATTT
    TTCTACTGCA AAGCATGTCA TGATGACATC ACAGATCCCA AACGGATTAA AAAGTGTGGC
    TGCAAGAGAA TGACATATTT CGAAGATGAG CATCCGTCAA CACTGTCACC CAAAAAGAAG
    CAGCGCAACG GAGGCATGCG AAATTCACCC AACTGCTCGC CCAAAATGAT GAGGCATGAT
    CCTCTACTCA TTCCAGGAAA TGAGCAGATT GAGAGCATGG ATGCTAATGT CAAACGATAC
    GACTCTACTG GAATGTTTCA CTGGTGTCCA TCCAAAGAAA TCGAGAAGGT CATTCTGACA
    CGCAGCGAGG CCTCAATGAC GGTTTTAAGT GGACATGTCG TAGTCTGCAT ATTTGGAGAT
    GTCACGTCAG CTTTAGTGGG GCTTCGAAAC TTAGTCATGC CTTTGAGAGC AAGCAACTTC
    CATTACCATG AGCTAAAGCC CATTGTGTTT GTGGGCTCGT TAGATTATCT GCGAAGAGAG
    TGGGAAACCC TTCACAACTT CCCCAAAGTC TTCATCTTAC CTGGTACGCC ATTAAGTCGA
    GCTGATTTAA GGGCCGTCAA CATCAACCTC TGCGACATGT GCGTTATCCT GTCAGCCAAT
    CAGAACAATA TCGACGATGC TTCACTGCAG GACAAGGAAT GCATCTTGGC ATCTCTTAAC
    ATCAAATCTA TGCAGTTTGA TGACAGCATT GGGGTCTTGC AGGCTAATTC CCAAGGTTTC
    ACACCTCCAG GGATGGATAG GTCATCTCCT GATAACAGTC CCGTGCACGG CTTAGTGCGG
    CAGGCATCCG TCACCACCGG ATCAAACATC CCCATCATAA CTGAACTGGT GAACGACTCC
    AACGTTCAGT TTTTGGACCA AGACGACGAT GACGACCCAG ACACTGAGCT CTACCTGACC
    CAGCCGTTCG CCTGTGGTAC CGCATTCGCC GTCAGCGTGT TGGACTCCCT GATGAGTGCG
    ACATACTTCA ATGATAATAT CCTCACCCTG ATCCGGACGC TGGTCACGGG AGGCGCCACG
    CCGGAGCTGG AGGCCCTGTT GGCTGAGGAG AACGCTCTGA GAGGTGGCTA CAGCACGCCG
    CAGACGCTGG CTAACAGGGA CAGGTGTCGG GTCGCCCAGC TGGCCCTCTA CGATGGGCCC
    TTCGCAGACT TGGGGGACGG AGGTTGCTAT GGAGACTTGT TCTGTAAAGC ATTAAAAACC
    TACAACATGC TGTGCTTTGG AATCTATAGA TTGCGAGATG CACATTTAGG TGCACCAAGC
    CAGTGCACAA AAAGATATGT CATCACAAAC CCGCCTTATG AGTTTGAGAT GGTGCCCACG
    GACCTGATCT TCTGTCTGAT GCAGTTCGAC CACAACGCGG GCCAGTCCCG AGCGAGCCTG
    TCCCACTCCT CCCATTCGTC CCATTCCTCC AGCAAGAAGA GCTCGTCTGT TCACTCCATC
    CCAGCCACCA ACCGGCAGAA CCGCAGCAGC AAAGCACGTG AAGCCCGTGA CAAACAGAAT
    GCAACAAGGA TGAATAGAAT GGGCCCAGAA AAGAGATGGT TTACAGATGA AGCAGAAAAC
    GCATACCCAA GGAACATTCA AATCAAGCCT ATGAGCACTC ACATGGCCAA CCAAGTCAAT
    CAGTACAAAT CCACTAGCAG TCTGATTCCA CCTATCCGAG AAGTCGAAGA CGAATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214276.1
    CDS84..2663
    Translation

    Target ORF information:

    RefSeq Version XM_017358787.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X29, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358787.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTTTGCTC GCTACGTGCC AGAAATTGCT GCTCTCATCC TTAATCGGAA GAAATACGGA 
    GGGAGTTATA ACTCGACACG AGGCAGAAAG CATATTGTGG TCTGTGGTCA TATCACGCTC
    GAAAGTGTTT CCAACTTCCT TAAAGACTTC CTACACAAGG ACAGGGATGA TGTCAATGTA
    GAAATTGTTT TTCTTCATAA TATTTCCCCT AATCTTGAGC TGGAAGCCTT GTTCAAACGT
    CACTTCACTC AGGTGGAATT CTATCAAGGA TCGGTCCTCA ACCCACATGA TCTAGCAAGA
    GTGAAGATTG AGTCGGCGGA CGCTTGCTTG ATTCTTGCAA ACAAATACTG TGCAGACCCC
    GATGCTGAGG ATGCCTCAAA TATCATGAGA GTGATATCTA TCAAGAACTA CCATCCAAAG
    ATCAGAATAA TAACACAAAT GTTGCAGTAC CACAACAAGG CCCATCTGCT AAACATTCCC
    AGCTGGAACT GGAAGGAGGG AGATGATGCG ATTTGCCTGG CCGAGTTGAA GGCAGGCTTC
    ATAGCCCAGA GCTGCCTGGC ACAGGGCCTG TCCACCATGC TCGCCAACCT CTTCTCCATG
    AGGTCATACA TCAAGATTGA AGAAGACACA TGGCAGAAGT ATTATCTGGA AGGAGTAGCC
    AATGAAATGT ACACTGAGTA CCTGTCTAGT GCCTTTGTCG GCCTTTCATT TCCTACAGTT
    TGTGAGCTGT GCTACGTGAA GCTCAAGTTG CTGTTGATCG CTATTGAGTA CAAGTCAGAG
    CAAAGGGAAA GCAGAAGCAG AAAGCGTATT CTGATCAACC CGGGAAATCA CGTTAAAATG
    CAGGAGGGGA CACTGGGATT CTTTATTGCC AGTGATGCCA AAGAAGTGAA AAGAGCATTT
    TTCTACTGCA AAGCATGTCA TGATGACATC ACAGATCCCA AACGGATTAA AAAGTGTGGC
    TGCAAGAGAA TGACATATTT CGAAGATGAG CATCCGTCAA CACTGTCACC CAAAAAGAAG
    CAGCGCAACG GAGGCATGCG AAATTCACCC AACTGCTCGC CCAAAATGAT GAGGCATGAT
    CCTCTACTCA TTCCAGGAAA TGAGCAGATT GAGAGCATGG ATGCTAATGT CAAACGATAC
    GACTCTACTG GAATGTTTCA CTGGTGTCCA TCCAAAGAAA TCGAGAAGGT CATTCTGACA
    CGCAGCGAGG CCTCAATGAC GGTTTTAAGT GGACATGTCG TAGTCTGCAT ATTTGGAGAT
    GTCACGTCAG CTTTAGTGGG GCTTCGAAAC TTAGTCATGC CTTTGAGAGC AAGCAACTTC
    CATTACCATG AGCTAAAGCC CATTGTGTTT GTGGGCTCGT TAGATTATCT GCGAAGAGAG
    TGGGAAACCC TTCACAACTT CCCCAAAGTC TTCATCTTAC CTGGTACGCC ATTAAGTCGA
    GCTGATTTAA GGGCCGTCAA CATCAACCTC TGCGACATGT GCGTTATCCT GTCAGCCAAT
    CAGAACAATA TCGACGATGC TTCACTGCAG GACAAGGAAT GCATCTTGGC ATCTCTTAAC
    ATCAAATCTA TGCAGTTTGA TGACAGCATT GGGGTCTTGC AGGCTAATTC CCAAGGTTTC
    ACACCTCCAG GGATGGATAG GTCATCTCCT GATAACAGTC CCGTGCACGG CTTAGTGCGG
    CAGGCATCCG TCACCACCGG ATCAAACATC CCCATCATAA CTGAACTGGT GAACGACTCC
    AACGTTCAGT TTTTGGACCA AGACGACGAT GACGACCCAG ACACTGAGCT CTACCTGACC
    CAGCCGTTCG CCTGTGGTAC CGCATTCGCC GTCAGCGTGT TGGACTCCCT GATGAGTGCG
    ACATACTTCA ATGATAATAT CCTCACCCTG ATCCGGACGC TGGTCACGGG AGGCGCCACG
    CCGGAGCTGG AGGCCCTGTT GGCTGAGGAG AACGCTCTGA GAGGTGGCTA CAGCACGCCG
    CAGACGCTGG CTAACAGGGA CAGGTGTCGG GTCGCCCAGC TGGCCCTCTA CGATGGGCCC
    TTCGCAGACT TGGGGGACGG AGGTTGCTAT GGAGACTTGT TCTGTAAAGC ATTAAAAACC
    TACAACATGC TGTGCTTTGG AATCTATAGA TTGCGAGATG CACATTTAGG TGCACCAAGC
    CAGTGCACAA AAAGATATGT CATCACAAAC CCGCCTTATG AGTTTGAGAT GGTGCCCACG
    GACCTGATCT TCTGTCTGAT GCAGTTCGAC CACAACGCGG GCCAGTCCCG AGCGAGCCTG
    TCCCACTCCT CCCATTCGTC CCATTCCTCC AGCAAGAAGA GCTCGTCTGT TCACTCCATC
    CCAGCCACCA ACCGGCAGAA CCGCAGCAGC AAAGCACGTG AAGCCCGTGA CAAACAGAAT
    GCAACAAGGA TGAATAGAAT GGGCCCAGAA AAGAGATGGT TTACAGATGA AGCAGAAAAC
    GCATACCCAA GGAACATTCA AATCAAGCCT ATGAGCACTC ACATGGCCAA CCAAGTCAAT
    CAGTACAAAT CCACTAGCAG TCTGATTCCA CCTATCCGAG AAGTCGAAGA CGAATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45455
    Clone ID Related Accession (Same CDS sequence) XM_017358788.1
    Accession Version XM_017358788.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2571bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X29
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTTTGCTC GCTACGTGCC AGAAATTGCT GCTCTCATCC TTAATCGGAA GAAATACGGA 
    GGGAGTTATA ACTCGACACG AGGCAGAAAG CATATTGTGG TCTGTGGTCA TATCACGCTC
    GAAAGTGTTT CCAACTTCCT TAAAGACTTC CTACACAAGG ACAGGGATGA TGTCAATGTA
    GAAATTGTTT TTCTTCATAA TATTTCCCCT AATCTTGAGC TGGAAGCCTT GTTCAAACGT
    CACTTCACTC AGGTGGAATT CTATCAAGGA TCGGTCCTCA ACCCACATGA TCTAGCAAGA
    GTGAAGATTG AGTCGGCGGA CGCTTGCTTG ATTCTTGCAA ACAAATACTG TGCAGACCCC
    GATGCTGAGG ATGCCTCAAA TATCATGAGA GTGATATCTA TCAAGAACTA CCATCCAAAG
    ATCAGAATAA TAACACAAAT GTTGCAGTAC CACAACAAGG CCCATCTGCT AAACATTCCC
    AGCTGGAACT GGAAGGAGGG AGATGATGCG ATTTGCCTGG CCGAGTTGAA GGCAGGCTTC
    ATAGCCCAGA GCTGCCTGGC ACAGGGCCTG TCCACCATGC TCGCCAACCT CTTCTCCATG
    AGGTCATACA TCAAGATTGA AGAAGACACA TGGCAGAAGT ATTATCTGGA AGGAGTAGCC
    AATGAAATGT ACACTGAGTA CCTGTCTAGT GCCTTTGTCG GCCTTTCATT TCCTACAGTT
    TGTGAGCTGT GCTACGTGAA GCTCAAGTTG CTGTTGATCG CTATTGAGTA CAAGTCAGAG
    CAAAGGGAAA GCAGAAGCAG AAAGCGTATT CTGATCAACC CGGGAAATCA CGTTAAAATG
    CAGGAGGGGA CACTGGGATT CTTTATTGCC AGTGATGCCA AAGAAGTGAA AAGAGCATTT
    TTCTACTGCA AAGCATGTCA TGATGACATC ACAGATCCCA AACGGATTAA AAAGTGTGGC
    TGCAAGAGAA TCGAAGATGA GCATCCGTCA ACACTGTCAC CCAAAAAGAA GCAGCGCAAC
    GGAGGCATGC GAAATTCACC CAACTGCTCG CCCAAAATGA TGAGGCATGA TCCTCTACTC
    ATTCCAGGAA ATGAGCAGAT TGAGAGCATG GATGCTAATG TCAAACGATA CGACTCTACT
    GGAATGTTTC ACTGGTGTCC ATCCAAAGAA ATCGAGAAGG TCATTCTGAC ACGCAGCGAG
    GCCTCAATGA CGGTTTTAAG TGGACATGTC GTAGTCTGCA TATTTGGAGA TGTCACGTCA
    GCTTTAGTGG GGCTTCGAAA CTTAGTCATG CCTTTGAGAG CAAGCAACTT CCATTACCAT
    GAGCTAAAGC CCATTGTGTT TGTGGGCTCG TTAGATTATC TGCGAAGAGA GTGGGAAACC
    CTTCACAACT TCCCCAAAGT CTTCATCTTA CCTGGTACGC CATTAAGTCG AGCTGATTTA
    AGGGCCGTCA ACATCAACCT CTGCGACATG TGCGTTATCC TGTCAGCCAA TCAGAACAAT
    ATCGACGATG CTTCACTGCA GGACAAGGAA TGCATCTTGG CATCTCTTAA CATCAAATCT
    ATGCAGTTTG ATGACAGCAT TGGGGTCTTG CAGGCTAATT CCCAAGGTTT CACACCTCCA
    GGGATGGATA GGTCATCTCC TGATAACAGT CCCGTGCACG GCTTAGTGCG GCAGGCATCC
    GTCACCACCG GATCAAACAT CCCCATCATA ACTGAACTGG TGAACGACTC CAACGTTCAG
    TTTTTGGACC AAGACGACGA TGACGACCCA GACACTGAGC TCTACCTGAC CCAGCCGTTC
    GCCTGTGGTA CCGCATTCGC CGTCAGCGTG TTGGACTCCC TGATGAGTGC GACATACTTC
    AATGATAATA TCCTCACCCT GATCCGGACG CTGGTCACGG GAGGCGCCAC GCCGGAGCTG
    GAGGCCCTGT TGGCTGAGGA GAACGCTCTG AGAGGTGGCT ACAGCACGCC GCAGACGCTG
    GCTAACAGGG ACAGGTGTCG GGTCGCCCAG CTGGCCCTCT ACGATGGGCC CTTCGCAGAC
    TTGGGGGACG GAGGTTGCTA TGGAGACTTG TTCTGTAAAG CATTAAAAAC CTACAACATG
    CTGTGCTTTG GAATCTATAG ATTGCGAGAT GCACATTTAG GTGCACCAAG CCAGTGCACA
    AAAAGATATG TCATCACAAA CCCGCCTTAT GAGTTTGAGA TGGTGCCCAC GGACCTGATC
    TTCTGTCTGA TGCAGTTCGA CCACAACGCG GGCCAGTCCC GAGCGAGCCT GTCCCACTCC
    TCCCATTCGT CCCATTCCTC CAGCAAGAAG AGCTCGTCTG TTCACTCCAT CCCAGCCACC
    AACCGGCAGA ACCGCAGCAG CAAAGCACGT GAAGCCCGTG ACAAACAGAA TGCAACAAGG
    ATGAATAGAA TGGGCCCAGA AAAGAGATGG TTTACAGATG AAGCAGAAAA CGCATACCCA
    AGGAACATTC AAATCAAGCC TATGAGCACT CACATGGCCA ACCAAGTCAA TCAGTACAAA
    TCCACTAGCA GTCTGATTCC ACCTATCCGA GAAGTCGAAG ACGAATGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214277.1
    CDS84..2654
    Translation

    Target ORF information:

    RefSeq Version XM_017358788.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X30, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358788.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTTTGCTC GCTACGTGCC AGAAATTGCT GCTCTCATCC TTAATCGGAA GAAATACGGA 
    GGGAGTTATA ACTCGACACG AGGCAGAAAG CATATTGTGG TCTGTGGTCA TATCACGCTC
    GAAAGTGTTT CCAACTTCCT TAAAGACTTC CTACACAAGG ACAGGGATGA TGTCAATGTA
    GAAATTGTTT TTCTTCATAA TATTTCCCCT AATCTTGAGC TGGAAGCCTT GTTCAAACGT
    CACTTCACTC AGGTGGAATT CTATCAAGGA TCGGTCCTCA ACCCACATGA TCTAGCAAGA
    GTGAAGATTG AGTCGGCGGA CGCTTGCTTG ATTCTTGCAA ACAAATACTG TGCAGACCCC
    GATGCTGAGG ATGCCTCAAA TATCATGAGA GTGATATCTA TCAAGAACTA CCATCCAAAG
    ATCAGAATAA TAACACAAAT GTTGCAGTAC CACAACAAGG CCCATCTGCT AAACATTCCC
    AGCTGGAACT GGAAGGAGGG AGATGATGCG ATTTGCCTGG CCGAGTTGAA GGCAGGCTTC
    ATAGCCCAGA GCTGCCTGGC ACAGGGCCTG TCCACCATGC TCGCCAACCT CTTCTCCATG
    AGGTCATACA TCAAGATTGA AGAAGACACA TGGCAGAAGT ATTATCTGGA AGGAGTAGCC
    AATGAAATGT ACACTGAGTA CCTGTCTAGT GCCTTTGTCG GCCTTTCATT TCCTACAGTT
    TGTGAGCTGT GCTACGTGAA GCTCAAGTTG CTGTTGATCG CTATTGAGTA CAAGTCAGAG
    CAAAGGGAAA GCAGAAGCAG AAAGCGTATT CTGATCAACC CGGGAAATCA CGTTAAAATG
    CAGGAGGGGA CACTGGGATT CTTTATTGCC AGTGATGCCA AAGAAGTGAA AAGAGCATTT
    TTCTACTGCA AAGCATGTCA TGATGACATC ACAGATCCCA AACGGATTAA AAAGTGTGGC
    TGCAAGAGAA TCGAAGATGA GCATCCGTCA ACACTGTCAC CCAAAAAGAA GCAGCGCAAC
    GGAGGCATGC GAAATTCACC CAACTGCTCG CCCAAAATGA TGAGGCATGA TCCTCTACTC
    ATTCCAGGAA ATGAGCAGAT TGAGAGCATG GATGCTAATG TCAAACGATA CGACTCTACT
    GGAATGTTTC ACTGGTGTCC ATCCAAAGAA ATCGAGAAGG TCATTCTGAC ACGCAGCGAG
    GCCTCAATGA CGGTTTTAAG TGGACATGTC GTAGTCTGCA TATTTGGAGA TGTCACGTCA
    GCTTTAGTGG GGCTTCGAAA CTTAGTCATG CCTTTGAGAG CAAGCAACTT CCATTACCAT
    GAGCTAAAGC CCATTGTGTT TGTGGGCTCG TTAGATTATC TGCGAAGAGA GTGGGAAACC
    CTTCACAACT TCCCCAAAGT CTTCATCTTA CCTGGTACGC CATTAAGTCG AGCTGATTTA
    AGGGCCGTCA ACATCAACCT CTGCGACATG TGCGTTATCC TGTCAGCCAA TCAGAACAAT
    ATCGACGATG CTTCACTGCA GGACAAGGAA TGCATCTTGG CATCTCTTAA CATCAAATCT
    ATGCAGTTTG ATGACAGCAT TGGGGTCTTG CAGGCTAATT CCCAAGGTTT CACACCTCCA
    GGGATGGATA GGTCATCTCC TGATAACAGT CCCGTGCACG GCTTAGTGCG GCAGGCATCC
    GTCACCACCG GATCAAACAT CCCCATCATA ACTGAACTGG TGAACGACTC CAACGTTCAG
    TTTTTGGACC AAGACGACGA TGACGACCCA GACACTGAGC TCTACCTGAC CCAGCCGTTC
    GCCTGTGGTA CCGCATTCGC CGTCAGCGTG TTGGACTCCC TGATGAGTGC GACATACTTC
    AATGATAATA TCCTCACCCT GATCCGGACG CTGGTCACGG GAGGCGCCAC GCCGGAGCTG
    GAGGCCCTGT TGGCTGAGGA GAACGCTCTG AGAGGTGGCT ACAGCACGCC GCAGACGCTG
    GCTAACAGGG ACAGGTGTCG GGTCGCCCAG CTGGCCCTCT ACGATGGGCC CTTCGCAGAC
    TTGGGGGACG GAGGTTGCTA TGGAGACTTG TTCTGTAAAG CATTAAAAAC CTACAACATG
    CTGTGCTTTG GAATCTATAG ATTGCGAGAT GCACATTTAG GTGCACCAAG CCAGTGCACA
    AAAAGATATG TCATCACAAA CCCGCCTTAT GAGTTTGAGA TGGTGCCCAC GGACCTGATC
    TTCTGTCTGA TGCAGTTCGA CCACAACGCG GGCCAGTCCC GAGCGAGCCT GTCCCACTCC
    TCCCATTCGT CCCATTCCTC CAGCAAGAAG AGCTCGTCTG TTCACTCCAT CCCAGCCACC
    AACCGGCAGA ACCGCAGCAG CAAAGCACGT GAAGCCCGTG ACAAACAGAA TGCAACAAGG
    ATGAATAGAA TGGGCCCAGA AAAGAGATGG TTTACAGATG AAGCAGAAAA CGCATACCCA
    AGGAACATTC AAATCAAGCC TATGAGCACT CACATGGCCA ACCAAGTCAA TCAGTACAAA
    TCCACTAGCA GTCTGATTCC ACCTATCCGA GAAGTCGAAG ACGAATGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45456
    Clone ID Related Accession (Same CDS sequence) XM_017358789.1
    Accession Version XM_017358789.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2568bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X30
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTTTGCTC GCTACGTGCC AGAAATTGCT GCTCTCATCC TTAATCGGAA GAAATACGGA 
    GGGAGTTATA ACTCGACACG AGGCAGAAAG CATATTGTGG TCTGTGGTCA TATCACGCTC
    GAAAGTGTTT CCAACTTCCT TAAAGACTTC CTACACAAGG ACAGGGATGA TGTCAATGTA
    GAAATTGTTT TTCTTCATAA TATTTCCCCT AATCTTGAGC TGGAAGCCTT GTTCAAACGT
    CACTTCACTC AGGTGGAATT CTATCAAGGA TCGGTCCTCA ACCCACATGA TCTAGCAAGA
    GTGAAGATTG AGTCGGCGGA CGCTTGCTTG ATTCTTGCAA ACAAATACTG TGCAGACCCC
    GATGCTGAGG ATGCCTCAAA TATCATGAGA GTGATATCTA TCAAGAACTA CCATCCAAAG
    ATCAGAATAA TAACACAAAT GTTGCAGTAC CACAACAAGG CCCATCTGCT AAACATTCCC
    AGCTGGAACT GGAAGGAGGG AGATGATGCG ATTTGCCTGG CCGAGTTGAA GGCAGGCTTC
    ATAGCCCAGA GCTGCCTGGC ACAGGGCCTG TCCACCATGC TCGCCAACCT CTTCTCCATG
    AGGTCATACA TCAAGATTGA AGAAGACACA TGGCAGAAGT ATTATCTGGA AGGAGTAGCC
    AATGAAATGT ACACTGAGTA CCTGTCTAGT GCCTTTGTCG GCCTTTCATT TCCTACAGTT
    TGTGAGCTGT GCTACGTGAA GCTCAAGTTG CTGTTGATCG CTATTGAGTA CAAGTCAGAG
    CAAAGGGAAA GCAGTATTCT GATCAACCCG GGAAATCACG TTAAAATGCA GGAGGGGACA
    CTGGGATTCT TTATTGCCAG TGATGCCAAA GAAGTGAAAA GAGCATTTTT CTACTGCAAA
    GCATGTCATG ATGACATCAC AGATCCCAAA CGGATTAAAA AGTGTGGCTG CAAGAGAATG
    ACATATTTCG AAGATGAGCA TCCGTCAACA CTGTCACCCA AAAAGAAGCA GCGCAACGGA
    GGCATGCGAA ATTCACCCAA CTGCTCGCCC AAAATGATGA GGCATGATCC TCTACTCATT
    CCAGGAAATG AGCAGATTGA GAGCATGGAT GCTAATGTCA AACGATACGA CTCTACTGGA
    ATGTTTCACT GGTGTCCATC CAAAGAAATC GAGAAGGTCA TTCTGACACG CAGCGAGGCC
    TCAATGACGG TTTTAAGTGG ACATGTCGTA GTCTGCATAT TTGGAGATGT CACGTCAGCT
    TTAGTGGGGC TTCGAAACTT AGTCATGCCT TTGAGAGCAA GCAACTTCCA TTACCATGAG
    CTAAAGCCCA TTGTGTTTGT GGGCTCGTTA GATTATCTGC GAAGAGAGTG GGAAACCCTT
    CACAACTTCC CCAAAGTCTT CATCTTACCT GGTACGCCAT TAAGTCGAGC TGATTTAAGG
    GCCGTCAACA TCAACCTCTG CGACATGTGC GTTATCCTGT CAGCCAATCA GAACAATATC
    GACGATGCTT CACTGCAGGA CAAGGAATGC ATCTTGGCAT CTCTTAACAT CAAATCTATG
    CAGTTTGATG ACAGCATTGG GGTCTTGCAG GCTAATTCCC AAGGTTTCAC ACCTCCAGGG
    ATGGATAGGT CATCTCCTGA TAACAGTCCC GTGCACGGCT TAGTGCGGCA GGCATCCGTC
    ACCACCGGAT CAAACATCCC CATCATAACT GAACTGGTGA ACGACTCCAA CGTTCAGTTT
    TTGGACCAAG ACGACGATGA CGACCCAGAC ACTGAGCTCT ACCTGACCCA GCCGTTCGCC
    TGTGGTACCG CATTCGCCGT CAGCGTGTTG GACTCCCTGA TGAGTGCGAC ATACTTCAAT
    GATAATATCC TCACCCTGAT CCGGACGCTG GTCACGGGAG GCGCCACGCC GGAGCTGGAG
    GCCCTGTTGG CTGAGGAGAA CGCTCTGAGA GGTGGCTACA GCACGCCGCA GACGCTGGCT
    AACAGGGACA GGTGTCGGGT CGCCCAGCTG GCCCTCTACG ATGGGCCCTT CGCAGACTTG
    GGGGACGGAG GTTGCTATGG AGACTTGTTC TGTAAAGCAT TAAAAACCTA CAACATGCTG
    TGCTTTGGAA TCTATAGATT GCGAGATGCA CATTTAGGTG CACCAAGCCA GTGCACAAAA
    AGATATGTCA TCACAAACCC GCCTTATGAG TTTGAGATGG TGCCCACGGA CCTGATCTTC
    TGTCTGATGC AGTTCGACCA CAACGCGGGC CAGTCCCGAG CGAGCCTGTC CCACTCCTCC
    CATTCGTCCC ATTCCTCCAG CAAGAAGAGC TCGTCTGTTC ACTCCATCCC AGCCACCAAC
    CGGCAGAACC GCAGCAGCAA AGCACGTGAA GCCCGTGACA AACAGAATGC AACAAGGATG
    AATAGAATGG GCCCAGAAAA GAGATGGTTT ACAGATGAAG CAGAAAACGC ATACCCAAGG
    AACATTCAAA TCAAGCCTAT GAGCACTCAC ATGGCCAACC AAGTCAATCA GTACAAATCC
    ACTAGCAGTC TGATTCCACC TATCCGAGAA GTCGAAGACG AATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214278.1
    CDS84..2651
    Translation

    Target ORF information:

    RefSeq Version XM_017358789.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X31, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358789.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTTTGCTC GCTACGTGCC AGAAATTGCT GCTCTCATCC TTAATCGGAA GAAATACGGA 
    GGGAGTTATA ACTCGACACG AGGCAGAAAG CATATTGTGG TCTGTGGTCA TATCACGCTC
    GAAAGTGTTT CCAACTTCCT TAAAGACTTC CTACACAAGG ACAGGGATGA TGTCAATGTA
    GAAATTGTTT TTCTTCATAA TATTTCCCCT AATCTTGAGC TGGAAGCCTT GTTCAAACGT
    CACTTCACTC AGGTGGAATT CTATCAAGGA TCGGTCCTCA ACCCACATGA TCTAGCAAGA
    GTGAAGATTG AGTCGGCGGA CGCTTGCTTG ATTCTTGCAA ACAAATACTG TGCAGACCCC
    GATGCTGAGG ATGCCTCAAA TATCATGAGA GTGATATCTA TCAAGAACTA CCATCCAAAG
    ATCAGAATAA TAACACAAAT GTTGCAGTAC CACAACAAGG CCCATCTGCT AAACATTCCC
    AGCTGGAACT GGAAGGAGGG AGATGATGCG ATTTGCCTGG CCGAGTTGAA GGCAGGCTTC
    ATAGCCCAGA GCTGCCTGGC ACAGGGCCTG TCCACCATGC TCGCCAACCT CTTCTCCATG
    AGGTCATACA TCAAGATTGA AGAAGACACA TGGCAGAAGT ATTATCTGGA AGGAGTAGCC
    AATGAAATGT ACACTGAGTA CCTGTCTAGT GCCTTTGTCG GCCTTTCATT TCCTACAGTT
    TGTGAGCTGT GCTACGTGAA GCTCAAGTTG CTGTTGATCG CTATTGAGTA CAAGTCAGAG
    CAAAGGGAAA GCAGTATTCT GATCAACCCG GGAAATCACG TTAAAATGCA GGAGGGGACA
    CTGGGATTCT TTATTGCCAG TGATGCCAAA GAAGTGAAAA GAGCATTTTT CTACTGCAAA
    GCATGTCATG ATGACATCAC AGATCCCAAA CGGATTAAAA AGTGTGGCTG CAAGAGAATG
    ACATATTTCG AAGATGAGCA TCCGTCAACA CTGTCACCCA AAAAGAAGCA GCGCAACGGA
    GGCATGCGAA ATTCACCCAA CTGCTCGCCC AAAATGATGA GGCATGATCC TCTACTCATT
    CCAGGAAATG AGCAGATTGA GAGCATGGAT GCTAATGTCA AACGATACGA CTCTACTGGA
    ATGTTTCACT GGTGTCCATC CAAAGAAATC GAGAAGGTCA TTCTGACACG CAGCGAGGCC
    TCAATGACGG TTTTAAGTGG ACATGTCGTA GTCTGCATAT TTGGAGATGT CACGTCAGCT
    TTAGTGGGGC TTCGAAACTT AGTCATGCCT TTGAGAGCAA GCAACTTCCA TTACCATGAG
    CTAAAGCCCA TTGTGTTTGT GGGCTCGTTA GATTATCTGC GAAGAGAGTG GGAAACCCTT
    CACAACTTCC CCAAAGTCTT CATCTTACCT GGTACGCCAT TAAGTCGAGC TGATTTAAGG
    GCCGTCAACA TCAACCTCTG CGACATGTGC GTTATCCTGT CAGCCAATCA GAACAATATC
    GACGATGCTT CACTGCAGGA CAAGGAATGC ATCTTGGCAT CTCTTAACAT CAAATCTATG
    CAGTTTGATG ACAGCATTGG GGTCTTGCAG GCTAATTCCC AAGGTTTCAC ACCTCCAGGG
    ATGGATAGGT CATCTCCTGA TAACAGTCCC GTGCACGGCT TAGTGCGGCA GGCATCCGTC
    ACCACCGGAT CAAACATCCC CATCATAACT GAACTGGTGA ACGACTCCAA CGTTCAGTTT
    TTGGACCAAG ACGACGATGA CGACCCAGAC ACTGAGCTCT ACCTGACCCA GCCGTTCGCC
    TGTGGTACCG CATTCGCCGT CAGCGTGTTG GACTCCCTGA TGAGTGCGAC ATACTTCAAT
    GATAATATCC TCACCCTGAT CCGGACGCTG GTCACGGGAG GCGCCACGCC GGAGCTGGAG
    GCCCTGTTGG CTGAGGAGAA CGCTCTGAGA GGTGGCTACA GCACGCCGCA GACGCTGGCT
    AACAGGGACA GGTGTCGGGT CGCCCAGCTG GCCCTCTACG ATGGGCCCTT CGCAGACTTG
    GGGGACGGAG GTTGCTATGG AGACTTGTTC TGTAAAGCAT TAAAAACCTA CAACATGCTG
    TGCTTTGGAA TCTATAGATT GCGAGATGCA CATTTAGGTG CACCAAGCCA GTGCACAAAA
    AGATATGTCA TCACAAACCC GCCTTATGAG TTTGAGATGG TGCCCACGGA CCTGATCTTC
    TGTCTGATGC AGTTCGACCA CAACGCGGGC CAGTCCCGAG CGAGCCTGTC CCACTCCTCC
    CATTCGTCCC ATTCCTCCAG CAAGAAGAGC TCGTCTGTTC ACTCCATCCC AGCCACCAAC
    CGGCAGAACC GCAGCAGCAA AGCACGTGAA GCCCGTGACA AACAGAATGC AACAAGGATG
    AATAGAATGG GCCCAGAAAA GAGATGGTTT ACAGATGAAG CAGAAAACGC ATACCCAAGG
    AACATTCAAA TCAAGCCTAT GAGCACTCAC ATGGCCAACC AAGTCAATCA GTACAAATCC
    ACTAGCAGTC TGATTCCACC TATCCGAGAA GTCGAAGACG AATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45457
    Clone ID Related Accession (Same CDS sequence) XM_017358790.1
    Accession Version XM_017358790.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2559bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X31
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTTTGCTC GCTACGTGCC AGAAATTGCT GCTCTCATCC TTAATCGGAA GAAATACGGA 
    GGGAGTTATA ACTCGACACG AGGCAGAAAG CATATTGTGG TCTGTGGTCA TATCACGCTC
    GAAAGTGTTT CCAACTTCCT TAAAGACTTC CTACACAAGG ACAGGGATGA TGTCAATGTA
    GAAATTGTTT TTCTTCATAA TATTTCCCCT AATCTTGAGC TGGAAGCCTT GTTCAAACGT
    CACTTCACTC AGGTGGAATT CTATCAAGGA TCGGTCCTCA ACCCACATGA TCTAGCAAGA
    GTGAAGATTG AGTCGGCGGA CGCTTGCTTG ATTCTTGCAA ACAAATACTG TGCAGACCCC
    GATGCTGAGG ATGCCTCAAA TATCATGAGA GTGATATCTA TCAAGAACTA CCATCCAAAG
    ATCAGAATAA TAACACAAAT GTTGCAGTAC CACAACAAGG CCCATCTGCT AAACATTCCC
    AGCTGGAACT GGAAGGAGGG AGATGATGCG ATTTGCCTGG CCGAGTTGAA GGCAGGCTTC
    ATAGCCCAGA GCTGCCTGGC ACAGGGCCTG TCCACCATGC TCGCCAACCT CTTCTCCATG
    AGGTCATACA TCAAGATTGA AGAAGACACA TGGCAGAAGT ATTATCTGGA AGGAGTAGCC
    AATGAAATGT ACACTGAGTA CCTGTCTAGT GCCTTTGTCG GCCTTTCATT TCCTACAGTT
    TGTGAGCTGT GCTACGTGAA GCTCAAGTTG CTGTTGATCG CTATTGAGTA CAAGTCAGAG
    CAAAGGGAAA GCAGTATTCT GATCAACCCG GGAAATCACG TTAAAATGCA GGAGGGGACA
    CTGGGATTCT TTATTGCCAG TGATGCCAAA GAAGTGAAAA GAGCATTTTT CTACTGCAAA
    GCATGTCATG ATGACATCAC AGATCCCAAA CGGATTAAAA AGTGTGGCTG CAAGAGAATC
    GAAGATGAGC ATCCGTCAAC ACTGTCACCC AAAAAGAAGC AGCGCAACGG AGGCATGCGA
    AATTCACCCA ACTGCTCGCC CAAAATGATG AGGCATGATC CTCTACTCAT TCCAGGAAAT
    GAGCAGATTG AGAGCATGGA TGCTAATGTC AAACGATACG ACTCTACTGG AATGTTTCAC
    TGGTGTCCAT CCAAAGAAAT CGAGAAGGTC ATTCTGACAC GCAGCGAGGC CTCAATGACG
    GTTTTAAGTG GACATGTCGT AGTCTGCATA TTTGGAGATG TCACGTCAGC TTTAGTGGGG
    CTTCGAAACT TAGTCATGCC TTTGAGAGCA AGCAACTTCC ATTACCATGA GCTAAAGCCC
    ATTGTGTTTG TGGGCTCGTT AGATTATCTG CGAAGAGAGT GGGAAACCCT TCACAACTTC
    CCCAAAGTCT TCATCTTACC TGGTACGCCA TTAAGTCGAG CTGATTTAAG GGCCGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAACAATAT CGACGATGCT
    TCACTGCAGG ACAAGGAATG CATCTTGGCA TCTCTTAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGTTTCA CACCTCCAGG GATGGATAGG
    TCATCTCCTG ATAACAGTCC CGTGCACGGC TTAGTGCGGC AGGCATCCGT CACCACCGGA
    TCAAACATCC CCATCATAAC TGAACTGGTG AACGACTCCA ACGTTCAGTT TTTGGACCAA
    GACGACGATG ACGACCCAGA CACTGAGCTC TACCTGACCC AGCCGTTCGC CTGTGGTACC
    GCATTCGCCG TCAGCGTGTT GGACTCCCTG ATGAGTGCGA CATACTTCAA TGATAATATC
    CTCACCCTGA TCCGGACGCT GGTCACGGGA GGCGCCACGC CGGAGCTGGA GGCCCTGTTG
    GCTGAGGAGA ACGCTCTGAG AGGTGGCTAC AGCACGCCGC AGACGCTGGC TAACAGGGAC
    AGGTGTCGGG TCGCCCAGCT GGCCCTCTAC GATGGGCCCT TCGCAGACTT GGGGGACGGA
    GGTTGCTATG GAGACTTGTT CTGTAAAGCA TTAAAAACCT ACAACATGCT GTGCTTTGGA
    ATCTATAGAT TGCGAGATGC ACATTTAGGT GCACCAAGCC AGTGCACAAA AAGATATGTC
    ATCACAAACC CGCCTTATGA GTTTGAGATG GTGCCCACGG ACCTGATCTT CTGTCTGATG
    CAGTTCGACC ACAACGCGGG CCAGTCCCGA GCGAGCCTGT CCCACTCCTC CCATTCGTCC
    CATTCCTCCA GCAAGAAGAG CTCGTCTGTT CACTCCATCC CAGCCACCAA CCGGCAGAAC
    CGCAGCAGCA AAGCACGTGA AGCCCGTGAC AAACAGAATG CAACAAGGAT GAATAGAATG
    GGCCCAGAAA AGAGATGGTT TACAGATGAA GCAGAAAACG CATACCCAAG GAACATTCAA
    ATCAAGCCTA TGAGCACTCA CATGGCCAAC CAAGTCAATC AGTACAAATC CACTAGCAGT
    CTGATTCCAC CTATCCGAGA AGTCGAAGAC GAATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214279.1
    CDS84..2642
    Translation

    Target ORF information:

    RefSeq Version XM_017358790.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X32, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358790.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGTTTGCTC GCTACGTGCC AGAAATTGCT GCTCTCATCC TTAATCGGAA GAAATACGGA 
    GGGAGTTATA ACTCGACACG AGGCAGAAAG CATATTGTGG TCTGTGGTCA TATCACGCTC
    GAAAGTGTTT CCAACTTCCT TAAAGACTTC CTACACAAGG ACAGGGATGA TGTCAATGTA
    GAAATTGTTT TTCTTCATAA TATTTCCCCT AATCTTGAGC TGGAAGCCTT GTTCAAACGT
    CACTTCACTC AGGTGGAATT CTATCAAGGA TCGGTCCTCA ACCCACATGA TCTAGCAAGA
    GTGAAGATTG AGTCGGCGGA CGCTTGCTTG ATTCTTGCAA ACAAATACTG TGCAGACCCC
    GATGCTGAGG ATGCCTCAAA TATCATGAGA GTGATATCTA TCAAGAACTA CCATCCAAAG
    ATCAGAATAA TAACACAAAT GTTGCAGTAC CACAACAAGG CCCATCTGCT AAACATTCCC
    AGCTGGAACT GGAAGGAGGG AGATGATGCG ATTTGCCTGG CCGAGTTGAA GGCAGGCTTC
    ATAGCCCAGA GCTGCCTGGC ACAGGGCCTG TCCACCATGC TCGCCAACCT CTTCTCCATG
    AGGTCATACA TCAAGATTGA AGAAGACACA TGGCAGAAGT ATTATCTGGA AGGAGTAGCC
    AATGAAATGT ACACTGAGTA CCTGTCTAGT GCCTTTGTCG GCCTTTCATT TCCTACAGTT
    TGTGAGCTGT GCTACGTGAA GCTCAAGTTG CTGTTGATCG CTATTGAGTA CAAGTCAGAG
    CAAAGGGAAA GCAGTATTCT GATCAACCCG GGAAATCACG TTAAAATGCA GGAGGGGACA
    CTGGGATTCT TTATTGCCAG TGATGCCAAA GAAGTGAAAA GAGCATTTTT CTACTGCAAA
    GCATGTCATG ATGACATCAC AGATCCCAAA CGGATTAAAA AGTGTGGCTG CAAGAGAATC
    GAAGATGAGC ATCCGTCAAC ACTGTCACCC AAAAAGAAGC AGCGCAACGG AGGCATGCGA
    AATTCACCCA ACTGCTCGCC CAAAATGATG AGGCATGATC CTCTACTCAT TCCAGGAAAT
    GAGCAGATTG AGAGCATGGA TGCTAATGTC AAACGATACG ACTCTACTGG AATGTTTCAC
    TGGTGTCCAT CCAAAGAAAT CGAGAAGGTC ATTCTGACAC GCAGCGAGGC CTCAATGACG
    GTTTTAAGTG GACATGTCGT AGTCTGCATA TTTGGAGATG TCACGTCAGC TTTAGTGGGG
    CTTCGAAACT TAGTCATGCC TTTGAGAGCA AGCAACTTCC ATTACCATGA GCTAAAGCCC
    ATTGTGTTTG TGGGCTCGTT AGATTATCTG CGAAGAGAGT GGGAAACCCT TCACAACTTC
    CCCAAAGTCT TCATCTTACC TGGTACGCCA TTAAGTCGAG CTGATTTAAG GGCCGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAACAATAT CGACGATGCT
    TCACTGCAGG ACAAGGAATG CATCTTGGCA TCTCTTAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGTTTCA CACCTCCAGG GATGGATAGG
    TCATCTCCTG ATAACAGTCC CGTGCACGGC TTAGTGCGGC AGGCATCCGT CACCACCGGA
    TCAAACATCC CCATCATAAC TGAACTGGTG AACGACTCCA ACGTTCAGTT TTTGGACCAA
    GACGACGATG ACGACCCAGA CACTGAGCTC TACCTGACCC AGCCGTTCGC CTGTGGTACC
    GCATTCGCCG TCAGCGTGTT GGACTCCCTG ATGAGTGCGA CATACTTCAA TGATAATATC
    CTCACCCTGA TCCGGACGCT GGTCACGGGA GGCGCCACGC CGGAGCTGGA GGCCCTGTTG
    GCTGAGGAGA ACGCTCTGAG AGGTGGCTAC AGCACGCCGC AGACGCTGGC TAACAGGGAC
    AGGTGTCGGG TCGCCCAGCT GGCCCTCTAC GATGGGCCCT TCGCAGACTT GGGGGACGGA
    GGTTGCTATG GAGACTTGTT CTGTAAAGCA TTAAAAACCT ACAACATGCT GTGCTTTGGA
    ATCTATAGAT TGCGAGATGC ACATTTAGGT GCACCAAGCC AGTGCACAAA AAGATATGTC
    ATCACAAACC CGCCTTATGA GTTTGAGATG GTGCCCACGG ACCTGATCTT CTGTCTGATG
    CAGTTCGACC ACAACGCGGG CCAGTCCCGA GCGAGCCTGT CCCACTCCTC CCATTCGTCC
    CATTCCTCCA GCAAGAAGAG CTCGTCTGTT CACTCCATCC CAGCCACCAA CCGGCAGAAC
    CGCAGCAGCA AAGCACGTGA AGCCCGTGAC AAACAGAATG CAACAAGGAT GAATAGAATG
    GGCCCAGAAA AGAGATGGTT TACAGATGAA GCAGAAAACG CATACCCAAG GAACATTCAA
    ATCAAGCCTA TGAGCACTCA CATGGCCAAC CAAGTCAATC AGTACAAATC CACTAGCAGT
    CTGATTCCAC CTATCCGAGA AGTCGAAGAC GAATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148543
    Clone ID Related Accession (Same CDS sequence) XM_021480644.1
    Accession Version XM_021480644.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2019bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X35
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCACACTC TGCTTCAGCA TGTCATCCTT CAATCATCTT CCTCATGTGT CGCTGTTTTT 
    GTCTTCCACT GTTTGACAGT AAAGGAGGAG GTTAGAGTGA GTTTTGAGAA GCGCTCAGTG
    AATGCGGTCA GCAGTGTGGC TGTTCCTCTG CCCCGTGTGC TGCTGGTTGT GGACTCCTCC
    ACGACTGCCG TTCCTCTTGT TAATAATCGT AAAGGAAGTT TGCTCCCTCC AGCGCCACCC
    TCGCTGTCTC TCCTCAGCCC GGCTGGCCTA AACCGGGCTC GCAGCCTGCC TGAAAAATAC
    CGCTACCGGC CCGGCAGCCG TTCCTCCGCA CACTTCGAAG ATGAGCATCC GTCAACACTG
    TCACCCAAAA AGAAGCAGCG CAACGGAGGC ATGCGAAATT CACCCAACTG CTCGCCCAAA
    ATGATGAGGC ATGATCCTCT ACTCATTCCA GGAAATGAGC AGATTGAGAG CATGGATGCT
    AATGTCAAAC GATACGACTC TACTGGAATG TTTCACTGGT GTCCATCCAA AGAAATCGAG
    AAGGTCATTC TGACACGCAG CGAGGCCTCA ATGACGGTTT TAAGTGGACA TGTCGTAGTC
    TGCATATTTG GAGATGTCAC GTCAGCTTTA GTGGGGCTTC GAAACTTAGT CATGCCTTTG
    AGAGCAAGCA ACTTCCATTA CCATGAGCTA AAGCCCATTG TGTTTGTGGG CTCGTTAGAT
    TATCTGCGAA GAGAGTGGGA AACCCTTCAC AACTTCCCCA AAGTCTTCAT CTTACCTGGT
    ACGCCATTAA GTCGAGCTGA TTTAAGGGCC GTCAACATCA ACCTCTGCGA CATGTGCGTT
    ATCCTGTCAG CCAATCAGAA CAATATCGAC GATGCTTCAC TGCAGGACAA GGAATGCATC
    TTGGCATCTC TTAACATCAA ATCTATGCAG TTTGATGACA GCATTGGGGT CTTGCAGGCT
    AATTCCCAAG GTTTCACACC TCCAGGGATG GATAGGTCAT CTCCTGATAA CAGTCCCGTG
    CACGGCTTAG TGCGGCAGGC ATCCGTCACC ACCGGATCAA ACATCCCCAT CATAACTGAA
    CTGGCTAAGC CTGGCAAACT ACTGCCTTTG GTTTCATTCA GTCAGGAAAA AAACAGTGGA
    ACCAACATAC AAATGATAAC AGAACTGGTG AACGACTCCA ACGTTCAGTT TTTGGACCAA
    GACGACGATG ACGACCCAGA CACTGAGCTC TACCTGACCC AGCCGTTCGC CTGTGGTACC
    GCATTCGCCG TCAGCGTGTT GGACTCCCTG ATGAGTGCGA CATACTTCAA TGATAATATC
    CTCACCCTGA TCCGGACGCT GGTCACGGGA GGCGCCACGC CGGAGCTGGA GGCCCTGTTG
    GCTGAGGAGA ACGCTCTGAG AGGTGGCTAC AGCACGCCGC AGACGCTGGC TAACAGGGAC
    AGGTGTCGGG TCGCCCAGCT GGCCCTCTAC GATGGGCCCT TCGCAGACTT GGGGGACGGA
    GGTTGCTATG GAGACTTGTT CTGTAAAGCA TTAAAAACCT ACAACATGCT GTGCTTTGGA
    ATCTATAGAT TGCGAGATGC ACATTTAGGT GCACCAAGCC AGTGCACAAA AAGATATGTC
    ATCACAAACC CGCCTTATGA GTTTGAGATG GTGCCCACGG ACCTGATCTT CTGTCTGATG
    CAGTTCGACC ACAACGCGGG CCAGTCCCGA GCGAGCCTGT CCCACTCCTC CCATTCGTCC
    CATTCCTCCA GCAAGAAGAG CTCGTCTGTT CACTCCATCC CAGCCACCAA CCGGCAGAAC
    CGCAGCAGCA AAGCACGTGA AGCCCGTGAC AAACAGAATG CAACAAGGAT GAATAGAATG
    GGCCCAGAAA AGAGATGGTT TACAGATGAA GCAGAAAACG CATACCCAAG GAACATTCAA
    ATCAAGCCTA TGAGCACTCA CATGGCCAAC CAAGTCAATC AGTACAAATC CACTAGCAGT
    CTGATTCCAC CTATCCGAGA AGTCGAAGAC GAATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336319.1
    CDS61..2079
    Translation

    Target ORF information:

    RefSeq Version XM_021480644.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X35, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480644.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCACACTC TGCTTCAGCA TGTCATCCTT CAATCATCTT CCTCATGTGT CGCTGTTTTT 
    GTCTTCCACT GTTTGACAGT AAAGGAGGAG GTTAGAGTGA GTTTTGAGAA GCGCTCAGTG
    AATGCGGTCA GCAGTGTGGC TGTTCCTCTG CCCCGTGTGC TGCTGGTTGT GGACTCCTCC
    ACGACTGCCG TTCCTCTTGT TAATAATCGT AAAGGAAGTT TGCTCCCTCC AGCGCCACCC
    TCGCTGTCTC TCCTCAGCCC GGCTGGCCTA AACCGGGCTC GCAGCCTGCC TGAAAAATAC
    CGCTACCGGC CCGGCAGCCG TTCCTCCGCA CACTTCGAAG ATGAGCATCC GTCAACACTG
    TCACCCAAAA AGAAGCAGCG CAACGGAGGC ATGCGAAATT CACCCAACTG CTCGCCCAAA
    ATGATGAGGC ATGATCCTCT ACTCATTCCA GGAAATGAGC AGATTGAGAG CATGGATGCT
    AATGTCAAAC GATACGACTC TACTGGAATG TTTCACTGGT GTCCATCCAA AGAAATCGAG
    AAGGTCATTC TGACACGCAG CGAGGCCTCA ATGACGGTTT TAAGTGGACA TGTCGTAGTC
    TGCATATTTG GAGATGTCAC GTCAGCTTTA GTGGGGCTTC GAAACTTAGT CATGCCTTTG
    AGAGCAAGCA ACTTCCATTA CCATGAGCTA AAGCCCATTG TGTTTGTGGG CTCGTTAGAT
    TATCTGCGAA GAGAGTGGGA AACCCTTCAC AACTTCCCCA AAGTCTTCAT CTTACCTGGT
    ACGCCATTAA GTCGAGCTGA TTTAAGGGCC GTCAACATCA ACCTCTGCGA CATGTGCGTT
    ATCCTGTCAG CCAATCAGAA CAATATCGAC GATGCTTCAC TGCAGGACAA GGAATGCATC
    TTGGCATCTC TTAACATCAA ATCTATGCAG TTTGATGACA GCATTGGGGT CTTGCAGGCT
    AATTCCCAAG GTTTCACACC TCCAGGGATG GATAGGTCAT CTCCTGATAA CAGTCCCGTG
    CACGGCTTAG TGCGGCAGGC ATCCGTCACC ACCGGATCAA ACATCCCCAT CATAACTGAA
    CTGGCTAAGC CTGGCAAACT ACTGCCTTTG GTTTCATTCA GTCAGGAAAA AAACAGTGGA
    ACCAACATAC AAATGATAAC AGAACTGGTG AACGACTCCA ACGTTCAGTT TTTGGACCAA
    GACGACGATG ACGACCCAGA CACTGAGCTC TACCTGACCC AGCCGTTCGC CTGTGGTACC
    GCATTCGCCG TCAGCGTGTT GGACTCCCTG ATGAGTGCGA CATACTTCAA TGATAATATC
    CTCACCCTGA TCCGGACGCT GGTCACGGGA GGCGCCACGC CGGAGCTGGA GGCCCTGTTG
    GCTGAGGAGA ACGCTCTGAG AGGTGGCTAC AGCACGCCGC AGACGCTGGC TAACAGGGAC
    AGGTGTCGGG TCGCCCAGCT GGCCCTCTAC GATGGGCCCT TCGCAGACTT GGGGGACGGA
    GGTTGCTATG GAGACTTGTT CTGTAAAGCA TTAAAAACCT ACAACATGCT GTGCTTTGGA
    ATCTATAGAT TGCGAGATGC ACATTTAGGT GCACCAAGCC AGTGCACAAA AAGATATGTC
    ATCACAAACC CGCCTTATGA GTTTGAGATG GTGCCCACGG ACCTGATCTT CTGTCTGATG
    CAGTTCGACC ACAACGCGGG CCAGTCCCGA GCGAGCCTGT CCCACTCCTC CCATTCGTCC
    CATTCCTCCA GCAAGAAGAG CTCGTCTGTT CACTCCATCC CAGCCACCAA CCGGCAGAAC
    CGCAGCAGCA AAGCACGTGA AGCCCGTGAC AAACAGAATG CAACAAGGAT GAATAGAATG
    GGCCCAGAAA AGAGATGGTT TACAGATGAA GCAGAAAACG CATACCCAAG GAACATTCAA
    ATCAAGCCTA TGAGCACTCA CATGGCCAAC CAAGTCAATC AGTACAAATC CACTAGCAGT
    CTGATTCCAC CTATCCGAGA AGTCGAAGAC GAATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54732
    Clone ID Related Accession (Same CDS sequence) XM_017358791.1
    Accession Version XM_017358791.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2400bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X32
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTTTGCTC GCTACGTGCC AGAAATTGCT GCTCTCATCC TTAATCGGAA GAAATACGGA 
    GGGAGTTATA ACTCGACACG AGGCAGAAAG CATATTGTGG TCTGTGGTCA TATCACGCTC
    GAAAGTGTTT CCAACTTCCT TAAAGACTTC CTACACAAGG ACAGGGATGA TGTCAATGTA
    GAAATTGTTT TTCTTCATAA TATTTCCCCT AATCTTGAGC TGGAAGCCTT GTTCAAACGT
    CACTTCACTC AGGTGGAATT CTATCAAGGA TCGGTCCTCA ACCCACATGA TCTAGCAAGA
    GTGAAGATTG AGTCGGCGGA CGCTTGCTTG ATTCTTGCAA ACAAATACTG TGCAGACCCC
    GATGCTGAGG ATGCCTCAAA TATCATGAGA GTGATATCTA TCAAGAACTA CCATCCAAAG
    ATCAGAATAA TAACACAAAT GTTGCAGTAC CACAACAAGG CCCATCTGCT AAACATTCCC
    AGCTGGAACT GGAAGGAGGG AGATGATGCG ATTTGCCTGG CCGAGTTGAA GGCAGGCTTC
    ATAGCCCAGA GCTGCCTGGC ACAGGGCCTG TCCACCATGC TCGCCAACCT CTTCTCCATG
    AGGTCATACA TCAAGATTGA AGAAGACACA TGGCAGAAGT ATTATCTGGA AGGAGTAGCC
    AATGAAATGT ACACTGAGTA CCTGTCTAGT GCCTTTGTCG GCCTTTCATT TCCTACAGTT
    TGTGAGCTGT GCTACGTGAA GCTCAAGTTG CTGTTGATCG CTATTGAGTA CAAGTCAGAG
    CAAAGGGAAA GCAGTATTCT GATCAACCCG GGAAATCACG TTAAAATGCA GGAGGGGACA
    CTGGGATTCT TTATTGCCAG TGATGCCAAA GAAGTGAAAA GAGCATTTTT CTACTGCAAA
    GCATGTCATG ATGACATCAC AGATCCCAAA CGGATTAAAA AGTGTGGCTG CAAGAGAATC
    GAAGATGAGC ATCCGTCAAC ACTGTCACCC AAAAAGAAGC AGCGCAACGG AGGCATGCGA
    AATTCACCCA ACTGCTCGCC CAAAATGATG AGGCATGATC CTCTACTCAT TCCAGGAAAT
    GAGCAGATTG AGAGCATGGA TGCTAATGTC AAACGATACG ACTCTACTGG AATGTTTCAC
    TGGTGTCCAT CCAAAGAAAT CGAGAAGGTC ATTCTGACAC GCAGCGAGGC CTCAATGACG
    GTTTTAAGTG GACATGTCGT AGTCTGCATA TTTGGAGATG TCACGTCAGC TTTAGTGGGG
    CTTCGAAACT TAGTCATGCC TTTGAGAGCA AGCAACTTCC ATTACCATGA GCTAAAGCCC
    ATTGTGTTTG TGGGCTCGTT AGATTATCTG CGAAGAGAGT GGGAAACCCT TCACAACTTC
    CCCAAAGTCT TCATCTTACC TGGTACGCCA TTAAGTCGAG CTGATTTAAG GGCCGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAACAATAT CGACGATGCT
    TCACTGCAGG ACAAGGAATG CATCTTGGCA TCTCTTAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGTTTCA CACCTCCAGG GATGGATAGG
    TCATCTCCTG ATAACAGTCC CGTGCACGGC TTAGTGCGGC AGGCATCCGT CACCACCGGA
    TCAAACATCC CCATCATAAC TGAACTGGTG AACGACTCCA ACGTTCAGTT TTTGGACCAA
    GACGACGATG ACGACCCAGA CACTGAGCTC TACCTGACCC AGCCGTTCGC CTGTGGTACC
    GCATTCGCCG TCAGCGTGTT GGACTCCCTG ATGAGTGCGA CATACTTCAA TGATAATATC
    CTCACCCTGA TCCGGACGCT GGTCACGGGA GGCGCCACGC CGGAGCTGGA GGCCCTGTTG
    GCTGAGGAGA ACGCTCTGAG AGGTGGCTAC AGCACGCCGC AGACGCTGGC TAACAGGGAC
    AGGTGTCGGG TCGCCCAGCT GGCCCTCTAC GATGGGCCCT TCGCAGACTT GGGGGACGGA
    GGTTGCTATG GAGACTTGTT CTGTAAAGCA TTAAAAACCT ACAACATGCT GTGCTTTGGA
    ATCTATAGAT TGCGAGATGC ACATTTAGGT GCACCAAGCC AGTGCACAAA AAGATATGTC
    ATCACAAACC CGCCTTATGA GTTTGAGATG GTGCCCACGG ACCTGATCTT CTGTCTGATG
    CAGTTCGACC ACAACGCGGG CCAGTCCCGA GCGAGCCTGT CCCACTCCTC CCATTCGTCC
    CATTCCTCCA GCAAGAAGAG CTCGTCTGTT CACTCCATCC CAGCCACCAA CCGGCAGAAC
    CGCAGCAGCA AAGCACGTGA AGCCCGTGAC AAACAGAAAA AAGAGATGGT TTACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214280.1
    CDS84..2483
    Translation

    Target ORF information:

    RefSeq Version XM_017358791.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X33, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358791.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGTTTGCTC GCTACGTGCC AGAAATTGCT GCTCTCATCC TTAATCGGAA GAAATACGGA 
    GGGAGTTATA ACTCGACACG AGGCAGAAAG CATATTGTGG TCTGTGGTCA TATCACGCTC
    GAAAGTGTTT CCAACTTCCT TAAAGACTTC CTACACAAGG ACAGGGATGA TGTCAATGTA
    GAAATTGTTT TTCTTCATAA TATTTCCCCT AATCTTGAGC TGGAAGCCTT GTTCAAACGT
    CACTTCACTC AGGTGGAATT CTATCAAGGA TCGGTCCTCA ACCCACATGA TCTAGCAAGA
    GTGAAGATTG AGTCGGCGGA CGCTTGCTTG ATTCTTGCAA ACAAATACTG TGCAGACCCC
    GATGCTGAGG ATGCCTCAAA TATCATGAGA GTGATATCTA TCAAGAACTA CCATCCAAAG
    ATCAGAATAA TAACACAAAT GTTGCAGTAC CACAACAAGG CCCATCTGCT AAACATTCCC
    AGCTGGAACT GGAAGGAGGG AGATGATGCG ATTTGCCTGG CCGAGTTGAA GGCAGGCTTC
    ATAGCCCAGA GCTGCCTGGC ACAGGGCCTG TCCACCATGC TCGCCAACCT CTTCTCCATG
    AGGTCATACA TCAAGATTGA AGAAGACACA TGGCAGAAGT ATTATCTGGA AGGAGTAGCC
    AATGAAATGT ACACTGAGTA CCTGTCTAGT GCCTTTGTCG GCCTTTCATT TCCTACAGTT
    TGTGAGCTGT GCTACGTGAA GCTCAAGTTG CTGTTGATCG CTATTGAGTA CAAGTCAGAG
    CAAAGGGAAA GCAGTATTCT GATCAACCCG GGAAATCACG TTAAAATGCA GGAGGGGACA
    CTGGGATTCT TTATTGCCAG TGATGCCAAA GAAGTGAAAA GAGCATTTTT CTACTGCAAA
    GCATGTCATG ATGACATCAC AGATCCCAAA CGGATTAAAA AGTGTGGCTG CAAGAGAATC
    GAAGATGAGC ATCCGTCAAC ACTGTCACCC AAAAAGAAGC AGCGCAACGG AGGCATGCGA
    AATTCACCCA ACTGCTCGCC CAAAATGATG AGGCATGATC CTCTACTCAT TCCAGGAAAT
    GAGCAGATTG AGAGCATGGA TGCTAATGTC AAACGATACG ACTCTACTGG AATGTTTCAC
    TGGTGTCCAT CCAAAGAAAT CGAGAAGGTC ATTCTGACAC GCAGCGAGGC CTCAATGACG
    GTTTTAAGTG GACATGTCGT AGTCTGCATA TTTGGAGATG TCACGTCAGC TTTAGTGGGG
    CTTCGAAACT TAGTCATGCC TTTGAGAGCA AGCAACTTCC ATTACCATGA GCTAAAGCCC
    ATTGTGTTTG TGGGCTCGTT AGATTATCTG CGAAGAGAGT GGGAAACCCT TCACAACTTC
    CCCAAAGTCT TCATCTTACC TGGTACGCCA TTAAGTCGAG CTGATTTAAG GGCCGTCAAC
    ATCAACCTCT GCGACATGTG CGTTATCCTG TCAGCCAATC AGAACAATAT CGACGATGCT
    TCACTGCAGG ACAAGGAATG CATCTTGGCA TCTCTTAACA TCAAATCTAT GCAGTTTGAT
    GACAGCATTG GGGTCTTGCA GGCTAATTCC CAAGGTTTCA CACCTCCAGG GATGGATAGG
    TCATCTCCTG ATAACAGTCC CGTGCACGGC TTAGTGCGGC AGGCATCCGT CACCACCGGA
    TCAAACATCC CCATCATAAC TGAACTGGTG AACGACTCCA ACGTTCAGTT TTTGGACCAA
    GACGACGATG ACGACCCAGA CACTGAGCTC TACCTGACCC AGCCGTTCGC CTGTGGTACC
    GCATTCGCCG TCAGCGTGTT GGACTCCCTG ATGAGTGCGA CATACTTCAA TGATAATATC
    CTCACCCTGA TCCGGACGCT GGTCACGGGA GGCGCCACGC CGGAGCTGGA GGCCCTGTTG
    GCTGAGGAGA ACGCTCTGAG AGGTGGCTAC AGCACGCCGC AGACGCTGGC TAACAGGGAC
    AGGTGTCGGG TCGCCCAGCT GGCCCTCTAC GATGGGCCCT TCGCAGACTT GGGGGACGGA
    GGTTGCTATG GAGACTTGTT CTGTAAAGCA TTAAAAACCT ACAACATGCT GTGCTTTGGA
    ATCTATAGAT TGCGAGATGC ACATTTAGGT GCACCAAGCC AGTGCACAAA AAGATATGTC
    ATCACAAACC CGCCTTATGA GTTTGAGATG GTGCCCACGG ACCTGATCTT CTGTCTGATG
    CAGTTCGACC ACAACGCGGG CCAGTCCCGA GCGAGCCTGT CCCACTCCTC CCATTCGTCC
    CATTCCTCCA GCAAGAAGAG CTCGTCTGTT CACTCCATCC CAGCCACCAA CCGGCAGAAC
    CGCAGCAGCA AAGCACGTGA AGCCCGTGAC AAACAGAAAA AAGAGATGGT TTACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148536
    Clone ID Related Accession (Same CDS sequence) XM_021480633.1
    Accession Version XM_021480633.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCTCGCT ACGTGCCAGA AATTGCTGCT
    CTCATCCTTA ATCGGAAGAA ATACGGAGGG AGTTATAACT CGACACGAGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT CCAAATATAA GTTTAACGGA
    TATGTCAGAT CACTCGAAGA TGAGCATCCG TCAACACTGT CACCCAAAAA GAAGCAGCGC
    AACGGAGGCA TGCGAAATTC ACCCAACTGC TCGCCCAAAA TGATGAGGCA TGATCCTCTA
    CTCATTCCAG GAAATGAGCA GATTGAGAGC ATGGATGCTA ATGTCAAACG ATACGACTCT
    ACTGGAATGT TTCACTGGTG TCCATCCAAA GAAATCGAGA AGGTCATTCT GACACGCAGC
    GAGGCCTCAA TGACGGTTTT AAGTGGACAT GTCGTAGTCT GCATATTTGG AGATGTCACG
    TCAGCTTTAG TGGGGCTTCG AAACTTAGTC ATGCCTTTGA GAGCAAGCAA CTTCCATTAC
    CATGAGCTAA AGCCCATTGT GTTTGTGGGC TCGTTAGATT ATCTGCGAAG AGAGTGGGAA
    ACCCTTCACA ACTTCCCCAA AGTCTTCATC TTACCTGGTA CGCCATTAAG TCGAGCTGAT
    TTAAGGGCCG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAC
    AATATCGACG ATGCTTCACT GCAGGACAAG GAATGCATCT TGGCATCTCT TAACATCAAA
    TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG TTTCACACCT
    CCAGGGATGG ATAGGTCATC TCCTGATAAC AGTCCCGTGC ACGGCTTAGT GCGGCAGGCA
    TCCGTCACCA CCGGATCAAA CATCCCCATC ATAACTGAAC TGGCTAAGCC TGGCAAACTA
    CTGCCTTTGG TTTCATTCAG TCAGGAAAAA AACAGTGGAA CCAACATACA AATGATAACA
    GAACTGGTGA ACGACTCCAA CGTTCAGTTT TTGGACCAAG ACGACGATGA CGACCCAGAC
    ACTGAGCTCT ACCTGACCCA GCCGTTCGCC TGTGGTACCG CATTCGCCGT CAGCGTGTTG
    GACTCCCTGA TGAGTGCGAC ATACTTCAAT GATAATATCC TCACCCTGAT CCGGACGCTG
    GTCACGGGAG GCGCCACGCC GGAGCTGGAG GCCCTGTTGG CTGAGGAGAA CGCTCTGAGA
    GGTGGCTACA GCACGCCGCA GACGCTGGCT AACAGGGACA GGTGTCGGGT CGCCCAGCTG
    GCCCTCTACG ATGGGCCCTT CGCAGACTTG GGGGACGGAG GTTGCTATGG AGACTTGTTC
    TGTAAAGCAT TAAAAACCTA CAACATGCTG TGCTTTGGAA TCTATAGATT GCGAGATGCA
    CATTTAGGTG CACCAAGCCA GTGCACAAAA AGATATGTCA TCACAAACCC GCCTTATGAG
    TTTGAGATGG TGCCCACGGA CCTGATCTTC TGTCTGATGC AGTTCGACCA CAACGCGGGC
    CAGTCCCGAG CGAGCCTGTC CCACTCCTCC CATTCGTCCC ATTCCTCCAG CAAGAAGAGC
    TCGTCTGTTC ACTCCATCCC AGCCACCAAC CGGCAGAACC GCAGCAGCAA AGCACGTGAA
    GCCCGTGACA AACAGAATGC AACAAGGATG AATAGAATGG GCCCAGAAAA GAGATGGTTT
    ACAGATGAAG CAGAAAACGC ATACCCAAGG AACATTCAAA TCAAGCCTAT GAGCACTCAC
    ATGGCCAACC AAGTCAATCA GTACAAATCC ACTAGCAGTC TGATTCCACC TATCCGAGAA
    GTCGAAGACG AATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336308.1
    CDS684..4601
    Translation

    Target ORF information:

    RefSeq Version XM_021480633.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480633.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCTCGCT ACGTGCCAGA AATTGCTGCT
    CTCATCCTTA ATCGGAAGAA ATACGGAGGG AGTTATAACT CGACACGAGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT CCAAATATAA GTTTAACGGA
    TATGTCAGAT CACTCGAAGA TGAGCATCCG TCAACACTGT CACCCAAAAA GAAGCAGCGC
    AACGGAGGCA TGCGAAATTC ACCCAACTGC TCGCCCAAAA TGATGAGGCA TGATCCTCTA
    CTCATTCCAG GAAATGAGCA GATTGAGAGC ATGGATGCTA ATGTCAAACG ATACGACTCT
    ACTGGAATGT TTCACTGGTG TCCATCCAAA GAAATCGAGA AGGTCATTCT GACACGCAGC
    GAGGCCTCAA TGACGGTTTT AAGTGGACAT GTCGTAGTCT GCATATTTGG AGATGTCACG
    TCAGCTTTAG TGGGGCTTCG AAACTTAGTC ATGCCTTTGA GAGCAAGCAA CTTCCATTAC
    CATGAGCTAA AGCCCATTGT GTTTGTGGGC TCGTTAGATT ATCTGCGAAG AGAGTGGGAA
    ACCCTTCACA ACTTCCCCAA AGTCTTCATC TTACCTGGTA CGCCATTAAG TCGAGCTGAT
    TTAAGGGCCG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAC
    AATATCGACG ATGCTTCACT GCAGGACAAG GAATGCATCT TGGCATCTCT TAACATCAAA
    TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG TTTCACACCT
    CCAGGGATGG ATAGGTCATC TCCTGATAAC AGTCCCGTGC ACGGCTTAGT GCGGCAGGCA
    TCCGTCACCA CCGGATCAAA CATCCCCATC ATAACTGAAC TGGCTAAGCC TGGCAAACTA
    CTGCCTTTGG TTTCATTCAG TCAGGAAAAA AACAGTGGAA CCAACATACA AATGATAACA
    GAACTGGTGA ACGACTCCAA CGTTCAGTTT TTGGACCAAG ACGACGATGA CGACCCAGAC
    ACTGAGCTCT ACCTGACCCA GCCGTTCGCC TGTGGTACCG CATTCGCCGT CAGCGTGTTG
    GACTCCCTGA TGAGTGCGAC ATACTTCAAT GATAATATCC TCACCCTGAT CCGGACGCTG
    GTCACGGGAG GCGCCACGCC GGAGCTGGAG GCCCTGTTGG CTGAGGAGAA CGCTCTGAGA
    GGTGGCTACA GCACGCCGCA GACGCTGGCT AACAGGGACA GGTGTCGGGT CGCCCAGCTG
    GCCCTCTACG ATGGGCCCTT CGCAGACTTG GGGGACGGAG GTTGCTATGG AGACTTGTTC
    TGTAAAGCAT TAAAAACCTA CAACATGCTG TGCTTTGGAA TCTATAGATT GCGAGATGCA
    CATTTAGGTG CACCAAGCCA GTGCACAAAA AGATATGTCA TCACAAACCC GCCTTATGAG
    TTTGAGATGG TGCCCACGGA CCTGATCTTC TGTCTGATGC AGTTCGACCA CAACGCGGGC
    CAGTCCCGAG CGAGCCTGTC CCACTCCTCC CATTCGTCCC ATTCCTCCAG CAAGAAGAGC
    TCGTCTGTTC ACTCCATCCC AGCCACCAAC CGGCAGAACC GCAGCAGCAA AGCACGTGAA
    GCCCGTGACA AACAGAATGC AACAAGGATG AATAGAATGG GCCCAGAAAA GAGATGGTTT
    ACAGATGAAG CAGAAAACGC ATACCCAAGG AACATTCAAA TCAAGCCTAT GAGCACTCAC
    ATGGCCAACC AAGTCAATCA GTACAAATCC ACTAGCAGTC TGATTCCACC TATCCGAGAA
    GTCGAAGACG AATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148537
    Clone ID Related Accession (Same CDS sequence) XM_021480635.1
    Accession Version XM_021480635.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3735bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAACCA AATATAAGTT TAACGGATAT
    GTCAGATCAC TCGAAGATGA GCATCCGTCA ACACTGTCAC CCAAAAAGAA GCAGCGCAAC
    GGAGGCATGC GAAATTCACC CAACTGCTCG CCCAAAATGA TGAGGCATGA TCCTCTACTC
    ATTCCAGGAA ATGAGCAGAT TGAGAGCATG GATGCTAATG TCAAACGATA CGACTCTACT
    GGAATGTTTC ACTGGTGTCC ATCCAAAGAA ATCGAGAAGG TCATTCTGAC ACGCAGCGAG
    GCCTCAATGA CGGTTTTAAG TGGACATGTC GTAGTCTGCA TATTTGGAGA TGTCACGTCA
    GCTTTAGTGG GGCTTCGAAA CTTAGTCATG CCTTTGAGAG CAAGCAACTT CCATTACCAT
    GAGCTAAAGC CCATTGTGTT TGTGGGCTCG TTAGATTATC TGCGAAGAGA GTGGGAAACC
    CTTCACAACT TCCCCAAAGT CTTCATCTTA CCTGGTACGC CATTAAGTCG AGCTGATTTA
    AGGGCCGTCA ACATCAACCT CTGCGACATG TGCGTTATCC TGTCAGCCAA TCAGAACAAT
    ATCGACGATG CTTCACTGCA GGACAAGGAA TGCATCTTGG CATCTCTTAA CATCAAATCT
    ATGCAGTTTG ATGACAGCAT TGGGGTCTTG CAGGCTAATT CCCAAGGTTT CACACCTCCA
    GGGATGGATA GGTCATCTCC TGATAACAGT CCCGTGCACG GCTTAGTGCG GCAGGCATCC
    GTCACCACCG GATCAAACAT CCCCATCATA ACTGAACTGG CTAAGCCTGG CAAACTACTG
    CCTTTGGTTT CATTCAGTCA GGAAAAAAAC AGTGGAACCA ACATACAAAT GATAACAGAA
    CTGGTGAACG ACTCCAACGT TCAGTTTTTG GACCAAGACG ACGATGACGA CCCAGACACT
    GAGCTCTACC TGACCCAGCC GTTCGCCTGT GGTACCGCAT TCGCCGTCAG CGTGTTGGAC
    TCCCTGATGA GTGCGACATA CTTCAATGAT AATATCCTCA CCCTGATCCG GACGCTGGTC
    ACGGGAGGCG CCACGCCGGA GCTGGAGGCC CTGTTGGCTG AGGAGAACGC TCTGAGAGGT
    GGCTACAGCA CGCCGCAGAC GCTGGCTAAC AGGGACAGGT GTCGGGTCGC CCAGCTGGCC
    CTCTACGATG GGCCCTTCGC AGACTTGGGG GACGGAGGTT GCTATGGAGA CTTGTTCTGT
    AAAGCATTAA AAACCTACAA CATGCTGTGC TTTGGAATCT ATAGATTGCG AGATGCACAT
    TTAGGTGCAC CAAGCCAGTG CACAAAAAGA TATGTCATCA CAAACCCGCC TTATGAGTTT
    GAGATGGTGC CCACGGACCT GATCTTCTGT CTGATGCAGT TCGACCACAA CGCGGGCCAG
    TCCCGAGCGA GCCTGTCCCA CTCCTCCCAT TCGTCCCATT CCTCCAGCAA GAAGAGCTCG
    TCTGTTCACT CCATCCCAGC CACCAACCGG CAGAACCGCA GCAGCAAAGC ACGTGAAGCC
    CGTGACAAAC AGAATGCAAC AAGGATGAAT AGAATGGGCC CAGAAAAGAG ATGGTTTACA
    GATGAAGCAG AAAACGCATA CCCAAGGAAC ATTCAAATCA AGCCTATGAG CACTCACATG
    GCCAACCAAG TCAATCAGTA CAAATCCACT AGCAGTCTGA TTCCACCTAT CCGAGAAGTC
    GAAGACGAAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336310.1
    CDS677..4411
    Translation

    Target ORF information:

    RefSeq Version XM_021480635.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480635.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAACCA AATATAAGTT TAACGGATAT
    GTCAGATCAC TCGAAGATGA GCATCCGTCA ACACTGTCAC CCAAAAAGAA GCAGCGCAAC
    GGAGGCATGC GAAATTCACC CAACTGCTCG CCCAAAATGA TGAGGCATGA TCCTCTACTC
    ATTCCAGGAA ATGAGCAGAT TGAGAGCATG GATGCTAATG TCAAACGATA CGACTCTACT
    GGAATGTTTC ACTGGTGTCC ATCCAAAGAA ATCGAGAAGG TCATTCTGAC ACGCAGCGAG
    GCCTCAATGA CGGTTTTAAG TGGACATGTC GTAGTCTGCA TATTTGGAGA TGTCACGTCA
    GCTTTAGTGG GGCTTCGAAA CTTAGTCATG CCTTTGAGAG CAAGCAACTT CCATTACCAT
    GAGCTAAAGC CCATTGTGTT TGTGGGCTCG TTAGATTATC TGCGAAGAGA GTGGGAAACC
    CTTCACAACT TCCCCAAAGT CTTCATCTTA CCTGGTACGC CATTAAGTCG AGCTGATTTA
    AGGGCCGTCA ACATCAACCT CTGCGACATG TGCGTTATCC TGTCAGCCAA TCAGAACAAT
    ATCGACGATG CTTCACTGCA GGACAAGGAA TGCATCTTGG CATCTCTTAA CATCAAATCT
    ATGCAGTTTG ATGACAGCAT TGGGGTCTTG CAGGCTAATT CCCAAGGTTT CACACCTCCA
    GGGATGGATA GGTCATCTCC TGATAACAGT CCCGTGCACG GCTTAGTGCG GCAGGCATCC
    GTCACCACCG GATCAAACAT CCCCATCATA ACTGAACTGG CTAAGCCTGG CAAACTACTG
    CCTTTGGTTT CATTCAGTCA GGAAAAAAAC AGTGGAACCA ACATACAAAT GATAACAGAA
    CTGGTGAACG ACTCCAACGT TCAGTTTTTG GACCAAGACG ACGATGACGA CCCAGACACT
    GAGCTCTACC TGACCCAGCC GTTCGCCTGT GGTACCGCAT TCGCCGTCAG CGTGTTGGAC
    TCCCTGATGA GTGCGACATA CTTCAATGAT AATATCCTCA CCCTGATCCG GACGCTGGTC
    ACGGGAGGCG CCACGCCGGA GCTGGAGGCC CTGTTGGCTG AGGAGAACGC TCTGAGAGGT
    GGCTACAGCA CGCCGCAGAC GCTGGCTAAC AGGGACAGGT GTCGGGTCGC CCAGCTGGCC
    CTCTACGATG GGCCCTTCGC AGACTTGGGG GACGGAGGTT GCTATGGAGA CTTGTTCTGT
    AAAGCATTAA AAACCTACAA CATGCTGTGC TTTGGAATCT ATAGATTGCG AGATGCACAT
    TTAGGTGCAC CAAGCCAGTG CACAAAAAGA TATGTCATCA CAAACCCGCC TTATGAGTTT
    GAGATGGTGC CCACGGACCT GATCTTCTGT CTGATGCAGT TCGACCACAA CGCGGGCCAG
    TCCCGAGCGA GCCTGTCCCA CTCCTCCCAT TCGTCCCATT CCTCCAGCAA GAAGAGCTCG
    TCTGTTCACT CCATCCCAGC CACCAACCGG CAGAACCGCA GCAGCAAAGC ACGTGAAGCC
    CGTGACAAAC AGAATGCAAC AAGGATGAAT AGAATGGGCC CAGAAAAGAG ATGGTTTACA
    GATGAAGCAG AAAACGCATA CCCAAGGAAC ATTCAAATCA AGCCTATGAG CACTCACATG
    GCCAACCAAG TCAATCAGTA CAAATCCACT AGCAGTCTGA TTCCACCTAT CCGAGAAGTC
    GAAGACGAAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148538
    Clone ID Related Accession (Same CDS sequence) XM_021480637.1
    Accession Version XM_021480637.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3627bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X21
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCTCGCT ACGTGCCAGA AATTGCTGCT
    CTCATCCTTA ATCGGAAGAA ATACGGAGGG AGTTATAACT CGACACGAGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTTCGA AGATGAGCAT
    CCGTCAACAC TGTCACCCAA AAAGAAGCAG CGCAACGGAG GCATGCGAAA TTCACCCAAC
    TGCTCGCCCA AAATGATGAG GCATGATCCT CTACTCATTC CAGGAAATGA GCAGATTGAG
    AGCATGGATG CTAATGTCAA ACGATACGAC TCTACTGGAA TGTTTCACTG GTGTCCATCC
    AAAGAAATCG AGAAGGTCAT TCTGACACGC AGCGAGGCCT CAATGACGGT TTTAAGTGGA
    CATGTCGTAG TCTGCATATT TGGAGATGTC ACGTCAGCTT TAGTGGGGCT TCGAAACTTA
    GTCATGCCTT TGAGAGCAAG CAACTTCCAT TACCATGAGC TAAAGCCCAT TGTGTTTGTG
    GGCTCGTTAG ATTATCTGCG AAGAGAGTGG GAAACCCTTC ACAACTTCCC CAAAGTCTTC
    ATCTTACCTG GTACGCCATT AAGTCGAGCT GATTTAAGGG CCGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AACAATATCG ACGATGCTTC ACTGCAGGAC
    AAGGAATGCA TCTTGGCATC TCTTAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGTTTCACA CCTCCAGGGA TGGATAGGTC ATCTCCTGAT
    AACAGTCCCG TGCACGGCTT AGTGCGGCAG GCATCCGTCA CCACCGGATC AAACATCCCC
    ATCATAACTG AACTGGTGAA CGACTCCAAC GTTCAGTTTT TGGACCAAGA CGACGATGAC
    GACCCAGACA CTGAGCTCTA CCTGACCCAG CCGTTCGCCT GTGGTACCGC ATTCGCCGTC
    AGCGTGTTGG ACTCCCTGAT GAGTGCGACA TACTTCAATG ATAATATCCT CACCCTGATC
    CGGACGCTGG TCACGGGAGG CGCCACGCCG GAGCTGGAGG CCCTGTTGGC TGAGGAGAAC
    GCTCTGAGAG GTGGCTACAG CACGCCGCAG ACGCTGGCTA ACAGGGACAG GTGTCGGGTC
    GCCCAGCTGG CCCTCTACGA TGGGCCCTTC GCAGACTTGG GGGACGGAGG TTGCTATGGA
    GACTTGTTCT GTAAAGCATT AAAAACCTAC AACATGCTGT GCTTTGGAAT CTATAGATTG
    CGAGATGCAC ATTTAGGTGC ACCAAGCCAG TGCACAAAAA GATATGTCAT CACAAACCCG
    CCTTATGAGT TTGAGATGGT GCCCACGGAC CTGATCTTCT GTCTGATGCA GTTCGACCAC
    AACGCGGGCC AGTCCCGAGC GAGCCTGTCC CACTCCTCCC ATTCGTCCCA TTCCTCCAGC
    AAGAAGAGCT CGTCTGTTCA CTCCATCCCA GCCACCAACC GGCAGAACCG CAGCAGCAAA
    GCACGTGAAG CCCGTGACAA ACAGAATGCA ACAAGGATGA ATAGAATGGG CCCAGAAAAG
    AGATGGTTTA CAGATGAAGC AGAAAACGCA TACCCAAGGA ACATTCAAAT CAAGCCTATG
    AGCACTCACA TGGCCAACCA AGTCAATCAG TACAAATCCA CTAGCAGTCT GATTCCACCT
    ATCCGAGAAG TCGAAGACGA ATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336312.1
    CDS677..4303
    Translation

    Target ORF information:

    RefSeq Version XM_021480637.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480637.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCTCGCT ACGTGCCAGA AATTGCTGCT
    CTCATCCTTA ATCGGAAGAA ATACGGAGGG AGTTATAACT CGACACGAGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTTCGA AGATGAGCAT
    CCGTCAACAC TGTCACCCAA AAAGAAGCAG CGCAACGGAG GCATGCGAAA TTCACCCAAC
    TGCTCGCCCA AAATGATGAG GCATGATCCT CTACTCATTC CAGGAAATGA GCAGATTGAG
    AGCATGGATG CTAATGTCAA ACGATACGAC TCTACTGGAA TGTTTCACTG GTGTCCATCC
    AAAGAAATCG AGAAGGTCAT TCTGACACGC AGCGAGGCCT CAATGACGGT TTTAAGTGGA
    CATGTCGTAG TCTGCATATT TGGAGATGTC ACGTCAGCTT TAGTGGGGCT TCGAAACTTA
    GTCATGCCTT TGAGAGCAAG CAACTTCCAT TACCATGAGC TAAAGCCCAT TGTGTTTGTG
    GGCTCGTTAG ATTATCTGCG AAGAGAGTGG GAAACCCTTC ACAACTTCCC CAAAGTCTTC
    ATCTTACCTG GTACGCCATT AAGTCGAGCT GATTTAAGGG CCGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AACAATATCG ACGATGCTTC ACTGCAGGAC
    AAGGAATGCA TCTTGGCATC TCTTAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGTTTCACA CCTCCAGGGA TGGATAGGTC ATCTCCTGAT
    AACAGTCCCG TGCACGGCTT AGTGCGGCAG GCATCCGTCA CCACCGGATC AAACATCCCC
    ATCATAACTG AACTGGTGAA CGACTCCAAC GTTCAGTTTT TGGACCAAGA CGACGATGAC
    GACCCAGACA CTGAGCTCTA CCTGACCCAG CCGTTCGCCT GTGGTACCGC ATTCGCCGTC
    AGCGTGTTGG ACTCCCTGAT GAGTGCGACA TACTTCAATG ATAATATCCT CACCCTGATC
    CGGACGCTGG TCACGGGAGG CGCCACGCCG GAGCTGGAGG CCCTGTTGGC TGAGGAGAAC
    GCTCTGAGAG GTGGCTACAG CACGCCGCAG ACGCTGGCTA ACAGGGACAG GTGTCGGGTC
    GCCCAGCTGG CCCTCTACGA TGGGCCCTTC GCAGACTTGG GGGACGGAGG TTGCTATGGA
    GACTTGTTCT GTAAAGCATT AAAAACCTAC AACATGCTGT GCTTTGGAAT CTATAGATTG
    CGAGATGCAC ATTTAGGTGC ACCAAGCCAG TGCACAAAAA GATATGTCAT CACAAACCCG
    CCTTATGAGT TTGAGATGGT GCCCACGGAC CTGATCTTCT GTCTGATGCA GTTCGACCAC
    AACGCGGGCC AGTCCCGAGC GAGCCTGTCC CACTCCTCCC ATTCGTCCCA TTCCTCCAGC
    AAGAAGAGCT CGTCTGTTCA CTCCATCCCA GCCACCAACC GGCAGAACCG CAGCAGCAAA
    GCACGTGAAG CCCGTGACAA ACAGAATGCA ACAAGGATGA ATAGAATGGG CCCAGAAAAG
    AGATGGTTTA CAGATGAAGC AGAAAACGCA TACCCAAGGA ACATTCAAAT CAAGCCTATG
    AGCACTCACA TGGCCAACCA AGTCAATCAG TACAAATCCA CTAGCAGTCT GATTCCACCT
    ATCCGAGAAG TCGAAGACGA ATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148539
    Clone ID Related Accession (Same CDS sequence) XM_021480639.1
    Accession Version XM_021480639.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X24
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCTCGCT ACGTGCCAGA AATTGCTGCT
    CTCATCCTTA ATCGGAAGAA ATACGGAGGG AGTTATAACT CGACACGAGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATCG AAGATGAGCA TCCGTCAACA
    CTGTCACCCA AAAAGAAGCA GCGCAACGGA GGCATGCGAA ATTCACCCAA CTGCTCGCCC
    AAAATGATGA GGCATGATCC TCTACTCATT CCAGGAAATG AGCAGATTGA GAGCATGGAT
    GCTAATGTCA AACGATACGA CTCTACTGGA ATGTTTCACT GGTGTCCATC CAAAGAAATC
    GAGAAGGTCA TTCTGACACG CAGCGAGGCC TCAATGACGG TTTTAAGTGG ACATGTCGTA
    GTCTGCATAT TTGGAGATGT CACGTCAGCT TTAGTGGGGC TTCGAAACTT AGTCATGCCT
    TTGAGAGCAA GCAACTTCCA TTACCATGAG CTAAAGCCCA TTGTGTTTGT GGGCTCGTTA
    GATTATCTGC GAAGAGAGTG GGAAACCCTT CACAACTTCC CCAAAGTCTT CATCTTACCT
    GGTACGCCAT TAAGTCGAGC TGATTTAAGG GCCGTCAACA TCAACCTCTG CGACATGTGC
    GTTATCCTGT CAGCCAATCA GAACAATATC GACGATGCTT CACTGCAGGA CAAGGAATGC
    ATCTTGGCAT CTCTTAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG
    GCTAATTCCC AAGGTTTCAC ACCTCCAGGG ATGGATAGGT CATCTCCTGA TAACAGTCCC
    GTGCACGGCT TAGTGCGGCA GGCATCCGTC ACCACCGGAT CAAACATCCC CATCATAACT
    GAACTGGTGA ACGACTCCAA CGTTCAGTTT TTGGACCAAG ACGACGATGA CGACCCAGAC
    ACTGAGCTCT ACCTGACCCA GCCGTTCGCC TGTGGTACCG CATTCGCCGT CAGCGTGTTG
    GACTCCCTGA TGAGTGCGAC ATACTTCAAT GATAATATCC TCACCCTGAT CCGGACGCTG
    GTCACGGGAG GCGCCACGCC GGAGCTGGAG GCCCTGTTGG CTGAGGAGAA CGCTCTGAGA
    GGTGGCTACA GCACGCCGCA GACGCTGGCT AACAGGGACA GGTGTCGGGT CGCCCAGCTG
    GCCCTCTACG ATGGGCCCTT CGCAGACTTG GGGGACGGAG GTTGCTATGG AGACTTGTTC
    TGTAAAGCAT TAAAAACCTA CAACATGCTG TGCTTTGGAA TCTATAGATT GCGAGATGCA
    CATTTAGGTG CACCAAGCCA GTGCACAAAA AGATATGTCA TCACAAACCC GCCTTATGAG
    TTTGAGATGG TGCCCACGGA CCTGATCTTC TGTCTGATGC AGTTCGACCA CAACGCGGGC
    CAGTCCCGAG CGAGCCTGTC CCACTCCTCC CATTCGTCCC ATTCCTCCAG CAAGAAGAGC
    TCGTCTGTTC ACTCCATCCC AGCCACCAAC CGGCAGAACC GCAGCAGCAA AGCACGTGAA
    GCCCGTGACA AACAGAATGC AACAAGGATG AATAGAATGG GCCCAGAAAA GAGATGGTTT
    ACAGATGAAG CAGAAAACGC ATACCCAAGG AACATTCAAA TCAAGCCTAT GAGCACTCAC
    ATGGCCAACC AAGTCAATCA GTACAAATCC ACTAGCAGTC TGATTCCACC TATCCGAGAA
    GTCGAAGACG AATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336314.1
    CDS677..4294
    Translation

    Target ORF information:

    RefSeq Version XM_021480639.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480639.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCTCGCT ACGTGCCAGA AATTGCTGCT
    CTCATCCTTA ATCGGAAGAA ATACGGAGGG AGTTATAACT CGACACGAGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATCG AAGATGAGCA TCCGTCAACA
    CTGTCACCCA AAAAGAAGCA GCGCAACGGA GGCATGCGAA ATTCACCCAA CTGCTCGCCC
    AAAATGATGA GGCATGATCC TCTACTCATT CCAGGAAATG AGCAGATTGA GAGCATGGAT
    GCTAATGTCA AACGATACGA CTCTACTGGA ATGTTTCACT GGTGTCCATC CAAAGAAATC
    GAGAAGGTCA TTCTGACACG CAGCGAGGCC TCAATGACGG TTTTAAGTGG ACATGTCGTA
    GTCTGCATAT TTGGAGATGT CACGTCAGCT TTAGTGGGGC TTCGAAACTT AGTCATGCCT
    TTGAGAGCAA GCAACTTCCA TTACCATGAG CTAAAGCCCA TTGTGTTTGT GGGCTCGTTA
    GATTATCTGC GAAGAGAGTG GGAAACCCTT CACAACTTCC CCAAAGTCTT CATCTTACCT
    GGTACGCCAT TAAGTCGAGC TGATTTAAGG GCCGTCAACA TCAACCTCTG CGACATGTGC
    GTTATCCTGT CAGCCAATCA GAACAATATC GACGATGCTT CACTGCAGGA CAAGGAATGC
    ATCTTGGCAT CTCTTAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG
    GCTAATTCCC AAGGTTTCAC ACCTCCAGGG ATGGATAGGT CATCTCCTGA TAACAGTCCC
    GTGCACGGCT TAGTGCGGCA GGCATCCGTC ACCACCGGAT CAAACATCCC CATCATAACT
    GAACTGGTGA ACGACTCCAA CGTTCAGTTT TTGGACCAAG ACGACGATGA CGACCCAGAC
    ACTGAGCTCT ACCTGACCCA GCCGTTCGCC TGTGGTACCG CATTCGCCGT CAGCGTGTTG
    GACTCCCTGA TGAGTGCGAC ATACTTCAAT GATAATATCC TCACCCTGAT CCGGACGCTG
    GTCACGGGAG GCGCCACGCC GGAGCTGGAG GCCCTGTTGG CTGAGGAGAA CGCTCTGAGA
    GGTGGCTACA GCACGCCGCA GACGCTGGCT AACAGGGACA GGTGTCGGGT CGCCCAGCTG
    GCCCTCTACG ATGGGCCCTT CGCAGACTTG GGGGACGGAG GTTGCTATGG AGACTTGTTC
    TGTAAAGCAT TAAAAACCTA CAACATGCTG TGCTTTGGAA TCTATAGATT GCGAGATGCA
    CATTTAGGTG CACCAAGCCA GTGCACAAAA AGATATGTCA TCACAAACCC GCCTTATGAG
    TTTGAGATGG TGCCCACGGA CCTGATCTTC TGTCTGATGC AGTTCGACCA CAACGCGGGC
    CAGTCCCGAG CGAGCCTGTC CCACTCCTCC CATTCGTCCC ATTCCTCCAG CAAGAAGAGC
    TCGTCTGTTC ACTCCATCCC AGCCACCAAC CGGCAGAACC GCAGCAGCAA AGCACGTGAA
    GCCCGTGACA AACAGAATGC AACAAGGATG AATAGAATGG GCCCAGAAAA GAGATGGTTT
    ACAGATGAAG CAGAAAACGC ATACCCAAGG AACATTCAAA TCAAGCCTAT GAGCACTCAC
    ATGGCCAACC AAGTCAATCA GTACAAATCC ACTAGCAGTC TGATTCCACC TATCCGAGAA
    GTCGAAGACG AATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148540
    Clone ID Related Accession (Same CDS sequence) XM_021480640.1
    Accession Version XM_021480640.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3615bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X26
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCTCGCT ACGTGCCAGA AATTGCTGCT
    CTCATCCTTA ATCGGAAGAA ATACGGAGGG AGTTATAACT CGACACGAGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATGACA TATTTCGAAG ATGAGCATCC GTCAACACTG
    TCACCCAAAA AGAAGCAGCG CAACGGAGGC ATGCGAAATT CACCCAACTG CTCGCCCAAA
    ATGATGAGGC ATGATCCTCT ACTCATTCCA GGAAATGAGC AGATTGAGAG CATGGATGCT
    AATGTCAAAC GATACGACTC TACTGGAATG TTTCACTGGT GTCCATCCAA AGAAATCGAG
    AAGGTCATTC TGACACGCAG CGAGGCCTCA ATGACGGTTT TAAGTGGACA TGTCGTAGTC
    TGCATATTTG GAGATGTCAC GTCAGCTTTA GTGGGGCTTC GAAACTTAGT CATGCCTTTG
    AGAGCAAGCA ACTTCCATTA CCATGAGCTA AAGCCCATTG TGTTTGTGGG CTCGTTAGAT
    TATCTGCGAA GAGAGTGGGA AACCCTTCAC AACTTCCCCA AAGTCTTCAT CTTACCTGGT
    ACGCCATTAA GTCGAGCTGA TTTAAGGGCC GTCAACATCA ACCTCTGCGA CATGTGCGTT
    ATCCTGTCAG CCAATCAGAA CAATATCGAC GATGCTTCAC TGCAGGACAA GGAATGCATC
    TTGGCATCTC TTAACATCAA ATCTATGCAG TTTGATGACA GCATTGGGGT CTTGCAGGCT
    AATTCCCAAG GTTTCACACC TCCAGGGATG GATAGGTCAT CTCCTGATAA CAGTCCCGTG
    CACGGCTTAG TGCGGCAGGC ATCCGTCACC ACCGGATCAA ACATCCCCAT CATAACTGAA
    CTGGTGAACG ACTCCAACGT TCAGTTTTTG GACCAAGACG ACGATGACGA CCCAGACACT
    GAGCTCTACC TGACCCAGCC GTTCGCCTGT GGTACCGCAT TCGCCGTCAG CGTGTTGGAC
    TCCCTGATGA GTGCGACATA CTTCAATGAT AATATCCTCA CCCTGATCCG GACGCTGGTC
    ACGGGAGGCG CCACGCCGGA GCTGGAGGCC CTGTTGGCTG AGGAGAACGC TCTGAGAGGT
    GGCTACAGCA CGCCGCAGAC GCTGGCTAAC AGGGACAGGT GTCGGGTCGC CCAGCTGGCC
    CTCTACGATG GGCCCTTCGC AGACTTGGGG GACGGAGGTT GCTATGGAGA CTTGTTCTGT
    AAAGCATTAA AAACCTACAA CATGCTGTGC TTTGGAATCT ATAGATTGCG AGATGCACAT
    TTAGGTGCAC CAAGCCAGTG CACAAAAAGA TATGTCATCA CAAACCCGCC TTATGAGTTT
    GAGATGGTGC CCACGGACCT GATCTTCTGT CTGATGCAGT TCGACCACAA CGCGGGCCAG
    TCCCGAGCGA GCCTGTCCCA CTCCTCCCAT TCGTCCCATT CCTCCAGCAA GAAGAGCTCG
    TCTGTTCACT CCATCCCAGC CACCAACCGG CAGAACCGCA GCAGCAAAGC ACGTGAAGCC
    CGTGACAAAC AGAATGCAAC AAGGATGAAT AGAATGGGCC CAGAAAAGAG ATGGTTTACA
    GATGAAGCAG AAAACGCATA CCCAAGGAAC ATTCAAATCA AGCCTATGAG CACTCACATG
    GCCAACCAAG TCAATCAGTA CAAATCCACT AGCAGTCTGA TTCCACCTAT CCGAGAAGTC
    GAAGACGAAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336315.1
    CDS676..4290
    Translation

    Target ORF information:

    RefSeq Version XM_021480640.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480640.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCTCGCT ACGTGCCAGA AATTGCTGCT
    CTCATCCTTA ATCGGAAGAA ATACGGAGGG AGTTATAACT CGACACGAGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATGACA TATTTCGAAG ATGAGCATCC GTCAACACTG
    TCACCCAAAA AGAAGCAGCG CAACGGAGGC ATGCGAAATT CACCCAACTG CTCGCCCAAA
    ATGATGAGGC ATGATCCTCT ACTCATTCCA GGAAATGAGC AGATTGAGAG CATGGATGCT
    AATGTCAAAC GATACGACTC TACTGGAATG TTTCACTGGT GTCCATCCAA AGAAATCGAG
    AAGGTCATTC TGACACGCAG CGAGGCCTCA ATGACGGTTT TAAGTGGACA TGTCGTAGTC
    TGCATATTTG GAGATGTCAC GTCAGCTTTA GTGGGGCTTC GAAACTTAGT CATGCCTTTG
    AGAGCAAGCA ACTTCCATTA CCATGAGCTA AAGCCCATTG TGTTTGTGGG CTCGTTAGAT
    TATCTGCGAA GAGAGTGGGA AACCCTTCAC AACTTCCCCA AAGTCTTCAT CTTACCTGGT
    ACGCCATTAA GTCGAGCTGA TTTAAGGGCC GTCAACATCA ACCTCTGCGA CATGTGCGTT
    ATCCTGTCAG CCAATCAGAA CAATATCGAC GATGCTTCAC TGCAGGACAA GGAATGCATC
    TTGGCATCTC TTAACATCAA ATCTATGCAG TTTGATGACA GCATTGGGGT CTTGCAGGCT
    AATTCCCAAG GTTTCACACC TCCAGGGATG GATAGGTCAT CTCCTGATAA CAGTCCCGTG
    CACGGCTTAG TGCGGCAGGC ATCCGTCACC ACCGGATCAA ACATCCCCAT CATAACTGAA
    CTGGTGAACG ACTCCAACGT TCAGTTTTTG GACCAAGACG ACGATGACGA CCCAGACACT
    GAGCTCTACC TGACCCAGCC GTTCGCCTGT GGTACCGCAT TCGCCGTCAG CGTGTTGGAC
    TCCCTGATGA GTGCGACATA CTTCAATGAT AATATCCTCA CCCTGATCCG GACGCTGGTC
    ACGGGAGGCG CCACGCCGGA GCTGGAGGCC CTGTTGGCTG AGGAGAACGC TCTGAGAGGT
    GGCTACAGCA CGCCGCAGAC GCTGGCTAAC AGGGACAGGT GTCGGGTCGC CCAGCTGGCC
    CTCTACGATG GGCCCTTCGC AGACTTGGGG GACGGAGGTT GCTATGGAGA CTTGTTCTGT
    AAAGCATTAA AAACCTACAA CATGCTGTGC TTTGGAATCT ATAGATTGCG AGATGCACAT
    TTAGGTGCAC CAAGCCAGTG CACAAAAAGA TATGTCATCA CAAACCCGCC TTATGAGTTT
    GAGATGGTGC CCACGGACCT GATCTTCTGT CTGATGCAGT TCGACCACAA CGCGGGCCAG
    TCCCGAGCGA GCCTGTCCCA CTCCTCCCAT TCGTCCCATT CCTCCAGCAA GAAGAGCTCG
    TCTGTTCACT CCATCCCAGC CACCAACCGG CAGAACCGCA GCAGCAAAGC ACGTGAAGCC
    CGTGACAAAC AGAATGCAAC AAGGATGAAT AGAATGGGCC CAGAAAAGAG ATGGTTTACA
    GATGAAGCAG AAAACGCATA CCCAAGGAAC ATTCAAATCA AGCCTATGAG CACTCACATG
    GCCAACCAAG TCAATCAGTA CAAATCCACT AGCAGTCTGA TTCCACCTAT CCGAGAAGTC
    GAAGACGAAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148541
    Clone ID Related Accession (Same CDS sequence) XM_021480641.1
    Accession Version XM_021480641.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3606bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCTCGCT ACGTGCCAGA AATTGCTGCT
    CTCATCCTTA ATCGGAAGAA ATACGGAGGG AGTTATAACT CGACACGAGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATCGAA GATGAGCATC CGTCAACACT GTCACCCAAA
    AAGAAGCAGC GCAACGGAGG CATGCGAAAT TCACCCAACT GCTCGCCCAA AATGATGAGG
    CATGATCCTC TACTCATTCC AGGAAATGAG CAGATTGAGA GCATGGATGC TAATGTCAAA
    CGATACGACT CTACTGGAAT GTTTCACTGG TGTCCATCCA AAGAAATCGA GAAGGTCATT
    CTGACACGCA GCGAGGCCTC AATGACGGTT TTAAGTGGAC ATGTCGTAGT CTGCATATTT
    GGAGATGTCA CGTCAGCTTT AGTGGGGCTT CGAAACTTAG TCATGCCTTT GAGAGCAAGC
    AACTTCCATT ACCATGAGCT AAAGCCCATT GTGTTTGTGG GCTCGTTAGA TTATCTGCGA
    AGAGAGTGGG AAACCCTTCA CAACTTCCCC AAAGTCTTCA TCTTACCTGG TACGCCATTA
    AGTCGAGCTG ATTTAAGGGC CGTCAACATC AACCTCTGCG ACATGTGCGT TATCCTGTCA
    GCCAATCAGA ACAATATCGA CGATGCTTCA CTGCAGGACA AGGAATGCAT CTTGGCATCT
    CTTAACATCA AATCTATGCA GTTTGATGAC AGCATTGGGG TCTTGCAGGC TAATTCCCAA
    GGTTTCACAC CTCCAGGGAT GGATAGGTCA TCTCCTGATA ACAGTCCCGT GCACGGCTTA
    GTGCGGCAGG CATCCGTCAC CACCGGATCA AACATCCCCA TCATAACTGA ACTGGTGAAC
    GACTCCAACG TTCAGTTTTT GGACCAAGAC GACGATGACG ACCCAGACAC TGAGCTCTAC
    CTGACCCAGC CGTTCGCCTG TGGTACCGCA TTCGCCGTCA GCGTGTTGGA CTCCCTGATG
    AGTGCGACAT ACTTCAATGA TAATATCCTC ACCCTGATCC GGACGCTGGT CACGGGAGGC
    GCCACGCCGG AGCTGGAGGC CCTGTTGGCT GAGGAGAACG CTCTGAGAGG TGGCTACAGC
    ACGCCGCAGA CGCTGGCTAA CAGGGACAGG TGTCGGGTCG CCCAGCTGGC CCTCTACGAT
    GGGCCCTTCG CAGACTTGGG GGACGGAGGT TGCTATGGAG ACTTGTTCTG TAAAGCATTA
    AAAACCTACA ACATGCTGTG CTTTGGAATC TATAGATTGC GAGATGCACA TTTAGGTGCA
    CCAAGCCAGT GCACAAAAAG ATATGTCATC ACAAACCCGC CTTATGAGTT TGAGATGGTG
    CCCACGGACC TGATCTTCTG TCTGATGCAG TTCGACCACA ACGCGGGCCA GTCCCGAGCG
    AGCCTGTCCC ACTCCTCCCA TTCGTCCCAT TCCTCCAGCA AGAAGAGCTC GTCTGTTCAC
    TCCATCCCAG CCACCAACCG GCAGAACCGC AGCAGCAAAG CACGTGAAGC CCGTGACAAA
    CAGAATGCAA CAAGGATGAA TAGAATGGGC CCAGAAAAGA GATGGTTTAC AGATGAAGCA
    GAAAACGCAT ACCCAAGGAA CATTCAAATC AAGCCTATGA GCACTCACAT GGCCAACCAA
    GTCAATCAGT ACAAATCCAC TAGCAGTCTG ATTCCACCTA TCCGAGAAGT CGAAGACGAA
    TGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336316.1
    CDS676..4281
    Translation

    Target ORF information:

    RefSeq Version XM_021480641.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X27, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480641.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCTCGCT ACGTGCCAGA AATTGCTGCT
    CTCATCCTTA ATCGGAAGAA ATACGGAGGG AGTTATAACT CGACACGAGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATCGAA GATGAGCATC CGTCAACACT GTCACCCAAA
    AAGAAGCAGC GCAACGGAGG CATGCGAAAT TCACCCAACT GCTCGCCCAA AATGATGAGG
    CATGATCCTC TACTCATTCC AGGAAATGAG CAGATTGAGA GCATGGATGC TAATGTCAAA
    CGATACGACT CTACTGGAAT GTTTCACTGG TGTCCATCCA AAGAAATCGA GAAGGTCATT
    CTGACACGCA GCGAGGCCTC AATGACGGTT TTAAGTGGAC ATGTCGTAGT CTGCATATTT
    GGAGATGTCA CGTCAGCTTT AGTGGGGCTT CGAAACTTAG TCATGCCTTT GAGAGCAAGC
    AACTTCCATT ACCATGAGCT AAAGCCCATT GTGTTTGTGG GCTCGTTAGA TTATCTGCGA
    AGAGAGTGGG AAACCCTTCA CAACTTCCCC AAAGTCTTCA TCTTACCTGG TACGCCATTA
    AGTCGAGCTG ATTTAAGGGC CGTCAACATC AACCTCTGCG ACATGTGCGT TATCCTGTCA
    GCCAATCAGA ACAATATCGA CGATGCTTCA CTGCAGGACA AGGAATGCAT CTTGGCATCT
    CTTAACATCA AATCTATGCA GTTTGATGAC AGCATTGGGG TCTTGCAGGC TAATTCCCAA
    GGTTTCACAC CTCCAGGGAT GGATAGGTCA TCTCCTGATA ACAGTCCCGT GCACGGCTTA
    GTGCGGCAGG CATCCGTCAC CACCGGATCA AACATCCCCA TCATAACTGA ACTGGTGAAC
    GACTCCAACG TTCAGTTTTT GGACCAAGAC GACGATGACG ACCCAGACAC TGAGCTCTAC
    CTGACCCAGC CGTTCGCCTG TGGTACCGCA TTCGCCGTCA GCGTGTTGGA CTCCCTGATG
    AGTGCGACAT ACTTCAATGA TAATATCCTC ACCCTGATCC GGACGCTGGT CACGGGAGGC
    GCCACGCCGG AGCTGGAGGC CCTGTTGGCT GAGGAGAACG CTCTGAGAGG TGGCTACAGC
    ACGCCGCAGA CGCTGGCTAA CAGGGACAGG TGTCGGGTCG CCCAGCTGGC CCTCTACGAT
    GGGCCCTTCG CAGACTTGGG GGACGGAGGT TGCTATGGAG ACTTGTTCTG TAAAGCATTA
    AAAACCTACA ACATGCTGTG CTTTGGAATC TATAGATTGC GAGATGCACA TTTAGGTGCA
    CCAAGCCAGT GCACAAAAAG ATATGTCATC ACAAACCCGC CTTATGAGTT TGAGATGGTG
    CCCACGGACC TGATCTTCTG TCTGATGCAG TTCGACCACA ACGCGGGCCA GTCCCGAGCG
    AGCCTGTCCC ACTCCTCCCA TTCGTCCCAT TCCTCCAGCA AGAAGAGCTC GTCTGTTCAC
    TCCATCCCAG CCACCAACCG GCAGAACCGC AGCAGCAAAG CACGTGAAGC CCGTGACAAA
    CAGAATGCAA CAAGGATGAA TAGAATGGGC CCAGAAAAGA GATGGTTTAC AGATGAAGCA
    GAAAACGCAT ACCCAAGGAA CATTCAAATC AAGCCTATGA GCACTCACAT GGCCAACCAA
    GTCAATCAGT ACAAATCCAC TAGCAGTCTG ATTCCACCTA TCCGAGAAGT CGAAGACGAA
    TGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa148542
    Clone ID Related Accession (Same CDS sequence) XM_021480642.1
    Accession Version XM_021480642.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3447bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X33
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCTCGCT ACGTGCCAGA AATTGCTGCT
    CTCATCCTTA ATCGGAAGAA ATACGGAGGG AGTTATAACT CGACACGAGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATCGAA GATGAGCATC CGTCAACACT GTCACCCAAA
    AAGAAGCAGC GCAACGGAGG CATGCGAAAT TCACCCAACT GCTCGCCCAA AATGATGAGG
    CATGATCCTC TACTCATTCC AGGAAATGAG CAGATTGAGA GCATGGATGC TAATGTCAAA
    CGATACGACT CTACTGGAAT GTTTCACTGG TGTCCATCCA AAGAAATCGA GAAGGTCATT
    CTGACACGCA GCGAGGCCTC AATGACGGTT TTAAGTGGAC ATGTCGTAGT CTGCATATTT
    GGAGATGTCA CGTCAGCTTT AGTGGGGCTT CGAAACTTAG TCATGCCTTT GAGAGCAAGC
    AACTTCCATT ACCATGAGCT AAAGCCCATT GTGTTTGTGG GCTCGTTAGA TTATCTGCGA
    AGAGAGTGGG AAACCCTTCA CAACTTCCCC AAAGTCTTCA TCTTACCTGG TACGCCATTA
    AGTCGAGCTG ATTTAAGGGC CGTCAACATC AACCTCTGCG ACATGTGCGT TATCCTGTCA
    GCCAATCAGA ACAATATCGA CGATGCTTCA CTGCAGGACA AGGAATGCAT CTTGGCATCT
    CTTAACATCA AATCTATGCA GTTTGATGAC AGCATTGGGG TCTTGCAGGC TAATTCCCAA
    GGTTTCACAC CTCCAGGGAT GGATAGGTCA TCTCCTGATA ACAGTCCCGT GCACGGCTTA
    GTGCGGCAGG CATCCGTCAC CACCGGATCA AACATCCCCA TCATAACTGA ACTGGTGAAC
    GACTCCAACG TTCAGTTTTT GGACCAAGAC GACGATGACG ACCCAGACAC TGAGCTCTAC
    CTGACCCAGC CGTTCGCCTG TGGTACCGCA TTCGCCGTCA GCGTGTTGGA CTCCCTGATG
    AGTGCGACAT ACTTCAATGA TAATATCCTC ACCCTGATCC GGACGCTGGT CACGGGAGGC
    GCCACGCCGG AGCTGGAGGC CCTGTTGGCT GAGGAGAACG CTCTGAGAGG TGGCTACAGC
    ACGCCGCAGA CGCTGGCTAA CAGGGACAGG TGTCGGGTCG CCCAGCTGGC CCTCTACGAT
    GGGCCCTTCG CAGACTTGGG GGACGGAGGT TGCTATGGAG ACTTGTTCTG TAAAGCATTA
    AAAACCTACA ACATGCTGTG CTTTGGAATC TATAGATTGC GAGATGCACA TTTAGGTGCA
    CCAAGCCAGT GCACAAAAAG ATATGTCATC ACAAACCCGC CTTATGAGTT TGAGATGGTG
    CCCACGGACC TGATCTTCTG TCTGATGCAG TTCGACCACA ACGCGGGCCA GTCCCGAGCG
    AGCCTGTCCC ACTCCTCCCA TTCGTCCCAT TCCTCCAGCA AGAAGAGCTC GTCTGTTCAC
    TCCATCCCAG CCACCAACCG GCAGAACCGC AGCAGCAAAG CACGTGAAGC CCGTGACAAA
    CAGAAAAAAG AGATGGTTTA CAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021336317.1
    CDS676..4122
    Translation

    Target ORF information:

    RefSeq Version XM_021480642.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X33, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021480642.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCTCGCT ACGTGCCAGA AATTGCTGCT
    CTCATCCTTA ATCGGAAGAA ATACGGAGGG AGTTATAACT CGACACGAGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATCGAA GATGAGCATC CGTCAACACT GTCACCCAAA
    AAGAAGCAGC GCAACGGAGG CATGCGAAAT TCACCCAACT GCTCGCCCAA AATGATGAGG
    CATGATCCTC TACTCATTCC AGGAAATGAG CAGATTGAGA GCATGGATGC TAATGTCAAA
    CGATACGACT CTACTGGAAT GTTTCACTGG TGTCCATCCA AAGAAATCGA GAAGGTCATT
    CTGACACGCA GCGAGGCCTC AATGACGGTT TTAAGTGGAC ATGTCGTAGT CTGCATATTT
    GGAGATGTCA CGTCAGCTTT AGTGGGGCTT CGAAACTTAG TCATGCCTTT GAGAGCAAGC
    AACTTCCATT ACCATGAGCT AAAGCCCATT GTGTTTGTGG GCTCGTTAGA TTATCTGCGA
    AGAGAGTGGG AAACCCTTCA CAACTTCCCC AAAGTCTTCA TCTTACCTGG TACGCCATTA
    AGTCGAGCTG ATTTAAGGGC CGTCAACATC AACCTCTGCG ACATGTGCGT TATCCTGTCA
    GCCAATCAGA ACAATATCGA CGATGCTTCA CTGCAGGACA AGGAATGCAT CTTGGCATCT
    CTTAACATCA AATCTATGCA GTTTGATGAC AGCATTGGGG TCTTGCAGGC TAATTCCCAA
    GGTTTCACAC CTCCAGGGAT GGATAGGTCA TCTCCTGATA ACAGTCCCGT GCACGGCTTA
    GTGCGGCAGG CATCCGTCAC CACCGGATCA AACATCCCCA TCATAACTGA ACTGGTGAAC
    GACTCCAACG TTCAGTTTTT GGACCAAGAC GACGATGACG ACCCAGACAC TGAGCTCTAC
    CTGACCCAGC CGTTCGCCTG TGGTACCGCA TTCGCCGTCA GCGTGTTGGA CTCCCTGATG
    AGTGCGACAT ACTTCAATGA TAATATCCTC ACCCTGATCC GGACGCTGGT CACGGGAGGC
    GCCACGCCGG AGCTGGAGGC CCTGTTGGCT GAGGAGAACG CTCTGAGAGG TGGCTACAGC
    ACGCCGCAGA CGCTGGCTAA CAGGGACAGG TGTCGGGTCG CCCAGCTGGC CCTCTACGAT
    GGGCCCTTCG CAGACTTGGG GGACGGAGGT TGCTATGGAG ACTTGTTCTG TAAAGCATTA
    AAAACCTACA ACATGCTGTG CTTTGGAATC TATAGATTGC GAGATGCACA TTTAGGTGCA
    CCAAGCCAGT GCACAAAAAG ATATGTCATC ACAAACCCGC CTTATGAGTT TGAGATGGTG
    CCCACGGACC TGATCTTCTG TCTGATGCAG TTCGACCACA ACGCGGGCCA GTCCCGAGCG
    AGCCTGTCCC ACTCCTCCCA TTCGTCCCAT TCCTCCAGCA AGAAGAGCTC GTCTGTTCAC
    TCCATCCCAG CCACCAACCG GCAGAACCGC AGCAGCAAAG CACGTGAAGC CCGTGACAAA
    CAGAAAAAAG AGATGGTTTA CAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54726
    Clone ID Related Accession (Same CDS sequence) XM_017358780.1 , XM_017358780.2
    Accession Version XM_017358780.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3675bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X15
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT CCAAATATAA GTTTAACGGA
    TATGTCAGAT CACTCGAAGA TGAGCATCCG TCAACACTGT CACCCAAAAA GAAGCAGCGC
    AACGGAGGCA TGCGAAATTC ACCCAACTGC TCGCCCAAAA TGATGAGGCA TGATCCTCTA
    CTCATTCCAG GAAATGAGCA GATTGAGAGC ATGGATGCTA ATGTCAAACG ATACGACTCT
    ACTGGAATGT TTCACTGGTG TCCATCCAAA GAAATCGAGA AGGTCATTCT GACACGCAGC
    GAGGCCTCAA TGACGGTTTT AAGTGGACAT GTCGTAGTCT GCATATTTGG AGATGTCACG
    TCAGCTTTAG TGGGGCTTCG AAACTTAGTC ATGCCTTTGA GAGCAAGCAA CTTCCATTAC
    CATGAGCTAA AGCCCATTGT GTTTGTGGGC TCGTTAGATT ATCTGCGAAG AGAGTGGGAA
    ACCCTTCACA ACTTCCCCAA AGTCTTCATC TTACCTGGTA CGCCATTAAG TCGAGCTGAT
    TTAAGGGCCG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAC
    AATATCGACG ATGCTTCACT GCAGGACAAG GAATGCATCT TGGCATCTCT TAACATCAAA
    TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG TTTCACACCT
    CCAGGGATGG ATAGGTCATC TCCTGATAAC AGTCCCGTGC ACGGCTTAGT GCGGCAGGCA
    TCCGTCACCA CCGGATCAAA CATCCCCATC ATAACTGAAC TGGTGAACGA CTCCAACGTT
    CAGTTTTTGG ACCAAGACGA CGATGACGAC CCAGACACTG AGCTCTACCT GACCCAGCCG
    TTCGCCTGTG GTACCGCATT CGCCGTCAGC GTGTTGGACT CCCTGATGAG TGCGACATAC
    TTCAATGATA ATATCCTCAC CCTGATCCGG ACGCTGGTCA CGGGAGGCGC CACGCCGGAG
    CTGGAGGCCC TGTTGGCTGA GGAGAACGCT CTGAGAGGTG GCTACAGCAC GCCGCAGACG
    CTGGCTAACA GGGACAGGTG TCGGGTCGCC CAGCTGGCCC TCTACGATGG GCCCTTCGCA
    GACTTGGGGG ACGGAGGTTG CTATGGAGAC TTGTTCTGTA AAGCATTAAA AACCTACAAC
    ATGCTGTGCT TTGGAATCTA TAGATTGCGA GATGCACATT TAGGTGCACC AAGCCAGTGC
    ACAAAAAGAT ATGTCATCAC AAACCCGCCT TATGAGTTTG AGATGGTGCC CACGGACCTG
    ATCTTCTGTC TGATGCAGTT CGACCACAAC GCGGGCCAGT CCCGAGCGAG CCTGTCCCAC
    TCCTCCCATT CGTCCCATTC CTCCAGCAAG AAGAGCTCGT CTGTTCACTC CATCCCAGCC
    ACCAACCGGC AGAACCGCAG CAGCAAAGCA CGTGAAGCCC GTGACAAACA GAAAAAAGAG
    ATGGTTTACA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214269.1
    CDS659..4333
    Translation

    Target ORF information:

    RefSeq Version XM_017358780.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358780.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT CCAAATATAA GTTTAACGGA
    TATGTCAGAT CACTCGAAGA TGAGCATCCG TCAACACTGT CACCCAAAAA GAAGCAGCGC
    AACGGAGGCA TGCGAAATTC ACCCAACTGC TCGCCCAAAA TGATGAGGCA TGATCCTCTA
    CTCATTCCAG GAAATGAGCA GATTGAGAGC ATGGATGCTA ATGTCAAACG ATACGACTCT
    ACTGGAATGT TTCACTGGTG TCCATCCAAA GAAATCGAGA AGGTCATTCT GACACGCAGC
    GAGGCCTCAA TGACGGTTTT AAGTGGACAT GTCGTAGTCT GCATATTTGG AGATGTCACG
    TCAGCTTTAG TGGGGCTTCG AAACTTAGTC ATGCCTTTGA GAGCAAGCAA CTTCCATTAC
    CATGAGCTAA AGCCCATTGT GTTTGTGGGC TCGTTAGATT ATCTGCGAAG AGAGTGGGAA
    ACCCTTCACA ACTTCCCCAA AGTCTTCATC TTACCTGGTA CGCCATTAAG TCGAGCTGAT
    TTAAGGGCCG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAC
    AATATCGACG ATGCTTCACT GCAGGACAAG GAATGCATCT TGGCATCTCT TAACATCAAA
    TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG TTTCACACCT
    CCAGGGATGG ATAGGTCATC TCCTGATAAC AGTCCCGTGC ACGGCTTAGT GCGGCAGGCA
    TCCGTCACCA CCGGATCAAA CATCCCCATC ATAACTGAAC TGGTGAACGA CTCCAACGTT
    CAGTTTTTGG ACCAAGACGA CGATGACGAC CCAGACACTG AGCTCTACCT GACCCAGCCG
    TTCGCCTGTG GTACCGCATT CGCCGTCAGC GTGTTGGACT CCCTGATGAG TGCGACATAC
    TTCAATGATA ATATCCTCAC CCTGATCCGG ACGCTGGTCA CGGGAGGCGC CACGCCGGAG
    CTGGAGGCCC TGTTGGCTGA GGAGAACGCT CTGAGAGGTG GCTACAGCAC GCCGCAGACG
    CTGGCTAACA GGGACAGGTG TCGGGTCGCC CAGCTGGCCC TCTACGATGG GCCCTTCGCA
    GACTTGGGGG ACGGAGGTTG CTATGGAGAC TTGTTCTGTA AAGCATTAAA AACCTACAAC
    ATGCTGTGCT TTGGAATCTA TAGATTGCGA GATGCACATT TAGGTGCACC AAGCCAGTGC
    ACAAAAAGAT ATGTCATCAC AAACCCGCCT TATGAGTTTG AGATGGTGCC CACGGACCTG
    ATCTTCTGTC TGATGCAGTT CGACCACAAC GCGGGCCAGT CCCGAGCGAG CCTGTCCCAC
    TCCTCCCATT CGTCCCATTC CTCCAGCAAG AAGAGCTCGT CTGTTCACTC CATCCCAGCC
    ACCAACCGGC AGAACCGCAG CAGCAAAGCA CGTGAAGCCC GTGACAAACA GAAAAAAGAG
    ATGGTTTACA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54726
    Clone ID Related Accession (Same CDS sequence) XM_017358780.1 , XM_017358780.2
    Accession Version XM_017358780.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3675bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X17
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358780.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT CCAAATATAA GTTTAACGGA
    TATGTCAGAT CACTCGAAGA TGAGCATCCG TCAACACTGT CACCCAAAAA GAAGCAGCGC
    AACGGAGGCA TGCGAAATTC ACCCAACTGC TCGCCCAAAA TGATGAGGCA TGATCCTCTA
    CTCATTCCAG GAAATGAGCA GATTGAGAGC ATGGATGCTA ATGTCAAACG ATACGACTCT
    ACTGGAATGT TTCACTGGTG TCCATCCAAA GAAATCGAGA AGGTCATTCT GACACGCAGC
    GAGGCCTCAA TGACGGTTTT AAGTGGACAT GTCGTAGTCT GCATATTTGG AGATGTCACG
    TCAGCTTTAG TGGGGCTTCG AAACTTAGTC ATGCCTTTGA GAGCAAGCAA CTTCCATTAC
    CATGAGCTAA AGCCCATTGT GTTTGTGGGC TCGTTAGATT ATCTGCGAAG AGAGTGGGAA
    ACCCTTCACA ACTTCCCCAA AGTCTTCATC TTACCTGGTA CGCCATTAAG TCGAGCTGAT
    TTAAGGGCCG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAC
    AATATCGACG ATGCTTCACT GCAGGACAAG GAATGCATCT TGGCATCTCT TAACATCAAA
    TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG TTTCACACCT
    CCAGGGATGG ATAGGTCATC TCCTGATAAC AGTCCCGTGC ACGGCTTAGT GCGGCAGGCA
    TCCGTCACCA CCGGATCAAA CATCCCCATC ATAACTGAAC TGGTGAACGA CTCCAACGTT
    CAGTTTTTGG ACCAAGACGA CGATGACGAC CCAGACACTG AGCTCTACCT GACCCAGCCG
    TTCGCCTGTG GTACCGCATT CGCCGTCAGC GTGTTGGACT CCCTGATGAG TGCGACATAC
    TTCAATGATA ATATCCTCAC CCTGATCCGG ACGCTGGTCA CGGGAGGCGC CACGCCGGAG
    CTGGAGGCCC TGTTGGCTGA GGAGAACGCT CTGAGAGGTG GCTACAGCAC GCCGCAGACG
    CTGGCTAACA GGGACAGGTG TCGGGTCGCC CAGCTGGCCC TCTACGATGG GCCCTTCGCA
    GACTTGGGGG ACGGAGGTTG CTATGGAGAC TTGTTCTGTA AAGCATTAAA AACCTACAAC
    ATGCTGTGCT TTGGAATCTA TAGATTGCGA GATGCACATT TAGGTGCACC AAGCCAGTGC
    ACAAAAAGAT ATGTCATCAC AAACCCGCCT TATGAGTTTG AGATGGTGCC CACGGACCTG
    ATCTTCTGTC TGATGCAGTT CGACCACAAC GCGGGCCAGT CCCGAGCGAG CCTGTCCCAC
    TCCTCCCATT CGTCCCATTC CTCCAGCAAG AAGAGCTCGT CTGTTCACTC CATCCCAGCC
    ACCAACCGGC AGAACCGCAG CAGCAAAGCA CGTGAAGCCC GTGACAAACA GAAAAAAGAG
    ATGGTTTACA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214269.1
    CDS677..4351
    Translation

    Target ORF information:

    RefSeq Version XM_017358780.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358780.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT CCAAATATAA GTTTAACGGA
    TATGTCAGAT CACTCGAAGA TGAGCATCCG TCAACACTGT CACCCAAAAA GAAGCAGCGC
    AACGGAGGCA TGCGAAATTC ACCCAACTGC TCGCCCAAAA TGATGAGGCA TGATCCTCTA
    CTCATTCCAG GAAATGAGCA GATTGAGAGC ATGGATGCTA ATGTCAAACG ATACGACTCT
    ACTGGAATGT TTCACTGGTG TCCATCCAAA GAAATCGAGA AGGTCATTCT GACACGCAGC
    GAGGCCTCAA TGACGGTTTT AAGTGGACAT GTCGTAGTCT GCATATTTGG AGATGTCACG
    TCAGCTTTAG TGGGGCTTCG AAACTTAGTC ATGCCTTTGA GAGCAAGCAA CTTCCATTAC
    CATGAGCTAA AGCCCATTGT GTTTGTGGGC TCGTTAGATT ATCTGCGAAG AGAGTGGGAA
    ACCCTTCACA ACTTCCCCAA AGTCTTCATC TTACCTGGTA CGCCATTAAG TCGAGCTGAT
    TTAAGGGCCG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAC
    AATATCGACG ATGCTTCACT GCAGGACAAG GAATGCATCT TGGCATCTCT TAACATCAAA
    TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG TTTCACACCT
    CCAGGGATGG ATAGGTCATC TCCTGATAAC AGTCCCGTGC ACGGCTTAGT GCGGCAGGCA
    TCCGTCACCA CCGGATCAAA CATCCCCATC ATAACTGAAC TGGTGAACGA CTCCAACGTT
    CAGTTTTTGG ACCAAGACGA CGATGACGAC CCAGACACTG AGCTCTACCT GACCCAGCCG
    TTCGCCTGTG GTACCGCATT CGCCGTCAGC GTGTTGGACT CCCTGATGAG TGCGACATAC
    TTCAATGATA ATATCCTCAC CCTGATCCGG ACGCTGGTCA CGGGAGGCGC CACGCCGGAG
    CTGGAGGCCC TGTTGGCTGA GGAGAACGCT CTGAGAGGTG GCTACAGCAC GCCGCAGACG
    CTGGCTAACA GGGACAGGTG TCGGGTCGCC CAGCTGGCCC TCTACGATGG GCCCTTCGCA
    GACTTGGGGG ACGGAGGTTG CTATGGAGAC TTGTTCTGTA AAGCATTAAA AACCTACAAC
    ATGCTGTGCT TTGGAATCTA TAGATTGCGA GATGCACATT TAGGTGCACC AAGCCAGTGC
    ACAAAAAGAT ATGTCATCAC AAACCCGCCT TATGAGTTTG AGATGGTGCC CACGGACCTG
    ATCTTCTGTC TGATGCAGTT CGACCACAAC GCGGGCCAGT CCCGAGCGAG CCTGTCCCAC
    TCCTCCCATT CGTCCCATTC CTCCAGCAAG AAGAGCTCGT CTGTTCACTC CATCCCAGCC
    ACCAACCGGC AGAACCGCAG CAGCAAAGCA CGTGAAGCCC GTGACAAACA GAAAAAAGAG
    ATGGTTTACA GATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54727
    Clone ID Related Accession (Same CDS sequence) XM_017358781.1 , XM_017358781.2
    Accession Version XM_017358781.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X22
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATCG AAGATGAGCA TCCGTCAACA
    CTGTCACCCA AAAAGAAGCA GCGCAACGGA GGCATGCGAA ATTCACCCAA CTGCTCGCCC
    AAAATGATGA GGCATGATCC TCTACTCATT CCAGGAAATG AGCAGATTGA GAGCATGGAT
    GCTAATGTCA AACGATACGA CTCTACTGGA ATGTTTCACT GGTGTCCATC CAAAGAAATC
    GAGAAGGTCA TTCTGACACG CAGCGAGGCC TCAATGACGG TTTTAAGTGG ACATGTCGTA
    GTCTGCATAT TTGGAGATGT CACGTCAGCT TTAGTGGGGC TTCGAAACTT AGTCATGCCT
    TTGAGAGCAA GCAACTTCCA TTACCATGAG CTAAAGCCCA TTGTGTTTGT GGGCTCGTTA
    GATTATCTGC GAAGAGAGTG GGAAACCCTT CACAACTTCC CCAAAGTCTT CATCTTACCT
    GGTACGCCAT TAAGTCGAGC TGATTTAAGG GCCGTCAACA TCAACCTCTG CGACATGTGC
    GTTATCCTGT CAGCCAATCA GAACAATATC GACGATGCTT CACTGCAGGA CAAGGAATGC
    ATCTTGGCAT CTCTTAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG
    GCTAATTCCC AAGGTTTCAC ACCTCCAGGG ATGGATAGGT CATCTCCTGA TAACAGTCCC
    GTGCACGGCT TAGTGCGGCA GGCATCCGTC ACCACCGGAT CAAACATCCC CATCATAACT
    GAACTGGCTA AGCCTGGCAA ACTACTGCCT TTGGTTTCAT TCAGTCAGGA AAAAAACAGT
    GGAACCAACA TACAAATGAT AACAGAACTG GTGAACGACT CCAACGTTCA GTTTTTGGAC
    CAAGACGACG ATGACGACCC AGACACTGAG CTCTACCTGA CCCAGCCGTT CGCCTGTGGT
    ACCGCATTCG CCGTCAGCGT GTTGGACTCC CTGATGAGTG CGACATACTT CAATGATAAT
    ATCCTCACCC TGATCCGGAC GCTGGTCACG GGAGGCGCCA CGCCGGAGCT GGAGGCCCTG
    TTGGCTGAGG AGAACGCTCT GAGAGGTGGC TACAGCACGC CGCAGACGCT GGCTAACAGG
    GACAGGTGTC GGGTCGCCCA GCTGGCCCTC TACGATGGGC CCTTCGCAGA CTTGGGGGAC
    GGAGGTTGCT ATGGAGACTT GTTCTGTAAA GCATTAAAAA CCTACAACAT GCTGTGCTTT
    GGAATCTATA GATTGCGAGA TGCACATTTA GGTGCACCAA GCCAGTGCAC AAAAAGATAT
    GTCATCACAA ACCCGCCTTA TGAGTTTGAG ATGGTGCCCA CGGACCTGAT CTTCTGTCTG
    ATGCAGTTCG ACCACAACGC GGGCCAGTCC CGAGCGAGCC TGTCCCACTC CTCCCATTCG
    TCCCATTCCT CCAGCAAGAA GAGCTCGTCT GTTCACTCCA TCCCAGCCAC CAACCGGCAG
    AACCGCAGCA GCAAAGCACG TGAAGCCCGT GACAAACAGA AAAAAGAGAT GGTTTACAGA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214270.1
    CDS659..4201
    Translation

    Target ORF information:

    RefSeq Version XM_017358781.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358781.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATCG AAGATGAGCA TCCGTCAACA
    CTGTCACCCA AAAAGAAGCA GCGCAACGGA GGCATGCGAA ATTCACCCAA CTGCTCGCCC
    AAAATGATGA GGCATGATCC TCTACTCATT CCAGGAAATG AGCAGATTGA GAGCATGGAT
    GCTAATGTCA AACGATACGA CTCTACTGGA ATGTTTCACT GGTGTCCATC CAAAGAAATC
    GAGAAGGTCA TTCTGACACG CAGCGAGGCC TCAATGACGG TTTTAAGTGG ACATGTCGTA
    GTCTGCATAT TTGGAGATGT CACGTCAGCT TTAGTGGGGC TTCGAAACTT AGTCATGCCT
    TTGAGAGCAA GCAACTTCCA TTACCATGAG CTAAAGCCCA TTGTGTTTGT GGGCTCGTTA
    GATTATCTGC GAAGAGAGTG GGAAACCCTT CACAACTTCC CCAAAGTCTT CATCTTACCT
    GGTACGCCAT TAAGTCGAGC TGATTTAAGG GCCGTCAACA TCAACCTCTG CGACATGTGC
    GTTATCCTGT CAGCCAATCA GAACAATATC GACGATGCTT CACTGCAGGA CAAGGAATGC
    ATCTTGGCAT CTCTTAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG
    GCTAATTCCC AAGGTTTCAC ACCTCCAGGG ATGGATAGGT CATCTCCTGA TAACAGTCCC
    GTGCACGGCT TAGTGCGGCA GGCATCCGTC ACCACCGGAT CAAACATCCC CATCATAACT
    GAACTGGCTA AGCCTGGCAA ACTACTGCCT TTGGTTTCAT TCAGTCAGGA AAAAAACAGT
    GGAACCAACA TACAAATGAT AACAGAACTG GTGAACGACT CCAACGTTCA GTTTTTGGAC
    CAAGACGACG ATGACGACCC AGACACTGAG CTCTACCTGA CCCAGCCGTT CGCCTGTGGT
    ACCGCATTCG CCGTCAGCGT GTTGGACTCC CTGATGAGTG CGACATACTT CAATGATAAT
    ATCCTCACCC TGATCCGGAC GCTGGTCACG GGAGGCGCCA CGCCGGAGCT GGAGGCCCTG
    TTGGCTGAGG AGAACGCTCT GAGAGGTGGC TACAGCACGC CGCAGACGCT GGCTAACAGG
    GACAGGTGTC GGGTCGCCCA GCTGGCCCTC TACGATGGGC CCTTCGCAGA CTTGGGGGAC
    GGAGGTTGCT ATGGAGACTT GTTCTGTAAA GCATTAAAAA CCTACAACAT GCTGTGCTTT
    GGAATCTATA GATTGCGAGA TGCACATTTA GGTGCACCAA GCCAGTGCAC AAAAAGATAT
    GTCATCACAA ACCCGCCTTA TGAGTTTGAG ATGGTGCCCA CGGACCTGAT CTTCTGTCTG
    ATGCAGTTCG ACCACAACGC GGGCCAGTCC CGAGCGAGCC TGTCCCACTC CTCCCATTCG
    TCCCATTCCT CCAGCAAGAA GAGCTCGTCT GTTCACTCCA TCCCAGCCAC CAACCGGCAG
    AACCGCAGCA GCAAAGCACG TGAAGCCCGT GACAAACAGA AAAAAGAGAT GGTTTACAGA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54727
    Clone ID Related Accession (Same CDS sequence) XM_017358781.1 , XM_017358781.2
    Accession Version XM_017358781.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3543bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X28
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358781.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATCG AAGATGAGCA TCCGTCAACA
    CTGTCACCCA AAAAGAAGCA GCGCAACGGA GGCATGCGAA ATTCACCCAA CTGCTCGCCC
    AAAATGATGA GGCATGATCC TCTACTCATT CCAGGAAATG AGCAGATTGA GAGCATGGAT
    GCTAATGTCA AACGATACGA CTCTACTGGA ATGTTTCACT GGTGTCCATC CAAAGAAATC
    GAGAAGGTCA TTCTGACACG CAGCGAGGCC TCAATGACGG TTTTAAGTGG ACATGTCGTA
    GTCTGCATAT TTGGAGATGT CACGTCAGCT TTAGTGGGGC TTCGAAACTT AGTCATGCCT
    TTGAGAGCAA GCAACTTCCA TTACCATGAG CTAAAGCCCA TTGTGTTTGT GGGCTCGTTA
    GATTATCTGC GAAGAGAGTG GGAAACCCTT CACAACTTCC CCAAAGTCTT CATCTTACCT
    GGTACGCCAT TAAGTCGAGC TGATTTAAGG GCCGTCAACA TCAACCTCTG CGACATGTGC
    GTTATCCTGT CAGCCAATCA GAACAATATC GACGATGCTT CACTGCAGGA CAAGGAATGC
    ATCTTGGCAT CTCTTAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG
    GCTAATTCCC AAGGTTTCAC ACCTCCAGGG ATGGATAGGT CATCTCCTGA TAACAGTCCC
    GTGCACGGCT TAGTGCGGCA GGCATCCGTC ACCACCGGAT CAAACATCCC CATCATAACT
    GAACTGGCTA AGCCTGGCAA ACTACTGCCT TTGGTTTCAT TCAGTCAGGA AAAAAACAGT
    GGAACCAACA TACAAATGAT AACAGAACTG GTGAACGACT CCAACGTTCA GTTTTTGGAC
    CAAGACGACG ATGACGACCC AGACACTGAG CTCTACCTGA CCCAGCCGTT CGCCTGTGGT
    ACCGCATTCG CCGTCAGCGT GTTGGACTCC CTGATGAGTG CGACATACTT CAATGATAAT
    ATCCTCACCC TGATCCGGAC GCTGGTCACG GGAGGCGCCA CGCCGGAGCT GGAGGCCCTG
    TTGGCTGAGG AGAACGCTCT GAGAGGTGGC TACAGCACGC CGCAGACGCT GGCTAACAGG
    GACAGGTGTC GGGTCGCCCA GCTGGCCCTC TACGATGGGC CCTTCGCAGA CTTGGGGGAC
    GGAGGTTGCT ATGGAGACTT GTTCTGTAAA GCATTAAAAA CCTACAACAT GCTGTGCTTT
    GGAATCTATA GATTGCGAGA TGCACATTTA GGTGCACCAA GCCAGTGCAC AAAAAGATAT
    GTCATCACAA ACCCGCCTTA TGAGTTTGAG ATGGTGCCCA CGGACCTGAT CTTCTGTCTG
    ATGCAGTTCG ACCACAACGC GGGCCAGTCC CGAGCGAGCC TGTCCCACTC CTCCCATTCG
    TCCCATTCCT CCAGCAAGAA GAGCTCGTCT GTTCACTCCA TCCCAGCCAC CAACCGGCAG
    AACCGCAGCA GCAAAGCACG TGAAGCCCGT GACAAACAGA AAAAAGAGAT GGTTTACAGA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214270.1
    CDS676..4218
    Translation

    Target ORF information:

    RefSeq Version XM_017358781.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X28, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358781.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATCG AAGATGAGCA TCCGTCAACA
    CTGTCACCCA AAAAGAAGCA GCGCAACGGA GGCATGCGAA ATTCACCCAA CTGCTCGCCC
    AAAATGATGA GGCATGATCC TCTACTCATT CCAGGAAATG AGCAGATTGA GAGCATGGAT
    GCTAATGTCA AACGATACGA CTCTACTGGA ATGTTTCACT GGTGTCCATC CAAAGAAATC
    GAGAAGGTCA TTCTGACACG CAGCGAGGCC TCAATGACGG TTTTAAGTGG ACATGTCGTA
    GTCTGCATAT TTGGAGATGT CACGTCAGCT TTAGTGGGGC TTCGAAACTT AGTCATGCCT
    TTGAGAGCAA GCAACTTCCA TTACCATGAG CTAAAGCCCA TTGTGTTTGT GGGCTCGTTA
    GATTATCTGC GAAGAGAGTG GGAAACCCTT CACAACTTCC CCAAAGTCTT CATCTTACCT
    GGTACGCCAT TAAGTCGAGC TGATTTAAGG GCCGTCAACA TCAACCTCTG CGACATGTGC
    GTTATCCTGT CAGCCAATCA GAACAATATC GACGATGCTT CACTGCAGGA CAAGGAATGC
    ATCTTGGCAT CTCTTAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG
    GCTAATTCCC AAGGTTTCAC ACCTCCAGGG ATGGATAGGT CATCTCCTGA TAACAGTCCC
    GTGCACGGCT TAGTGCGGCA GGCATCCGTC ACCACCGGAT CAAACATCCC CATCATAACT
    GAACTGGCTA AGCCTGGCAA ACTACTGCCT TTGGTTTCAT TCAGTCAGGA AAAAAACAGT
    GGAACCAACA TACAAATGAT AACAGAACTG GTGAACGACT CCAACGTTCA GTTTTTGGAC
    CAAGACGACG ATGACGACCC AGACACTGAG CTCTACCTGA CCCAGCCGTT CGCCTGTGGT
    ACCGCATTCG CCGTCAGCGT GTTGGACTCC CTGATGAGTG CGACATACTT CAATGATAAT
    ATCCTCACCC TGATCCGGAC GCTGGTCACG GGAGGCGCCA CGCCGGAGCT GGAGGCCCTG
    TTGGCTGAGG AGAACGCTCT GAGAGGTGGC TACAGCACGC CGCAGACGCT GGCTAACAGG
    GACAGGTGTC GGGTCGCCCA GCTGGCCCTC TACGATGGGC CCTTCGCAGA CTTGGGGGAC
    GGAGGTTGCT ATGGAGACTT GTTCTGTAAA GCATTAAAAA CCTACAACAT GCTGTGCTTT
    GGAATCTATA GATTGCGAGA TGCACATTTA GGTGCACCAA GCCAGTGCAC AAAAAGATAT
    GTCATCACAA ACCCGCCTTA TGAGTTTGAG ATGGTGCCCA CGGACCTGAT CTTCTGTCTG
    ATGCAGTTCG ACCACAACGC GGGCCAGTCC CGAGCGAGCC TGTCCCACTC CTCCCATTCG
    TCCCATTCCT CCAGCAAGAA GAGCTCGTCT GTTCACTCCA TCCCAGCCAC CAACCGGCAG
    AACCGCAGCA GCAAAGCACG TGAAGCCCGT GACAAACAGA AAAAAGAGAT GGTTTACAGA
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54728
    Clone ID Related Accession (Same CDS sequence) XM_017358782.2 , XM_017358782.1
    Accession Version XM_017358782.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3540bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X29
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358782.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATGACA TATTTCGAAG ATGAGCATCC GTCAACACTG
    TCACCCAAAA AGAAGCAGCG CAACGGAGGC ATGCGAAATT CACCCAACTG CTCGCCCAAA
    ATGATGAGGC ATGATCCTCT ACTCATTCCA GGAAATGAGC AGATTGAGAG CATGGATGCT
    AATGTCAAAC GATACGACTC TACTGGAATG TTTCACTGGT GTCCATCCAA AGAAATCGAG
    AAGGTCATTC TGACACGCAG CGAGGCCTCA ATGACGGTTT TAAGTGGACA TGTCGTAGTC
    TGCATATTTG GAGATGTCAC GTCAGCTTTA GTGGGGCTTC GAAACTTAGT CATGCCTTTG
    AGAGCAAGCA ACTTCCATTA CCATGAGCTA AAGCCCATTG TGTTTGTGGG CTCGTTAGAT
    TATCTGCGAA GAGAGTGGGA AACCCTTCAC AACTTCCCCA AAGTCTTCAT CTTACCTGGT
    ACGCCATTAA GTCGAGCTGA TTTAAGGGCC GTCAACATCA ACCTCTGCGA CATGTGCGTT
    ATCCTGTCAG CCAATCAGAA CAATATCGAC GATGCTTCAC TGCAGGACAA GGAATGCATC
    TTGGCATCTC TTAACATCAA ATCTATGCAG TTTGATGACA GCATTGGGGT CTTGCAGGCT
    AATTCCCAAG GTTTCACACC TCCAGGGATG GATAGGTCAT CTCCTGATAA CAGTCCCGTG
    CACGGCTTAG TGCGGCAGGC ATCCGTCACC ACCGGATCAA ACATCCCCAT CATAACTGAA
    CTGGCTAAGC CTGGCAAACT ACTGCCTTTG GTTTCATTCA GTCAGGAAAA AAACAGTGGA
    ACCAACATAC AAATGATAAC AGAACTGGTG AACGACTCCA ACGTTCAGTT TTTGGACCAA
    GACGACGATG ACGACCCAGA CACTGAGCTC TACCTGACCC AGCCGTTCGC CTGTGGTACC
    GCATTCGCCG TCAGCGTGTT GGACTCCCTG ATGAGTGCGA CATACTTCAA TGATAATATC
    CTCACCCTGA TCCGGACGCT GGTCACGGGA GGCGCCACGC CGGAGCTGGA GGCCCTGTTG
    GCTGAGGAGA ACGCTCTGAG AGGTGGCTAC AGCACGCCGC AGACGCTGGC TAACAGGGAC
    AGGTGTCGGG TCGCCCAGCT GGCCCTCTAC GATGGGCCCT TCGCAGACTT GGGGGACGGA
    GGTTGCTATG GAGACTTGTT CTGTAAAGCA TTAAAAACCT ACAACATGCT GTGCTTTGGA
    ATCTATAGAT TGCGAGATGC ACATTTAGGT GCACCAAGCC AGTGCACAAA AAGATATGTC
    ATCACAAACC CGCCTTATGA GTTTGAGATG GTGCCCACGG ACCTGATCTT CTGTCTGATG
    CAGTTCGACC ACAACGCGGG CCAGTCCCGA GCGAGCCTGT CCCACTCCTC CCATTCGTCC
    CATTCCTCCA GCAAGAAGAG CTCGTCTGTT CACTCCATCC CAGCCACCAA CCGGCAGAAC
    CGCAGCAGCA AAGCACGTGA AGCCCGTGAC AAACAGAAAA AAGAGATGGT TTACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214271.1
    CDS675..4214
    Translation

    Target ORF information:

    RefSeq Version XM_017358782.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X29, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358782.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATGACA TATTTCGAAG ATGAGCATCC GTCAACACTG
    TCACCCAAAA AGAAGCAGCG CAACGGAGGC ATGCGAAATT CACCCAACTG CTCGCCCAAA
    ATGATGAGGC ATGATCCTCT ACTCATTCCA GGAAATGAGC AGATTGAGAG CATGGATGCT
    AATGTCAAAC GATACGACTC TACTGGAATG TTTCACTGGT GTCCATCCAA AGAAATCGAG
    AAGGTCATTC TGACACGCAG CGAGGCCTCA ATGACGGTTT TAAGTGGACA TGTCGTAGTC
    TGCATATTTG GAGATGTCAC GTCAGCTTTA GTGGGGCTTC GAAACTTAGT CATGCCTTTG
    AGAGCAAGCA ACTTCCATTA CCATGAGCTA AAGCCCATTG TGTTTGTGGG CTCGTTAGAT
    TATCTGCGAA GAGAGTGGGA AACCCTTCAC AACTTCCCCA AAGTCTTCAT CTTACCTGGT
    ACGCCATTAA GTCGAGCTGA TTTAAGGGCC GTCAACATCA ACCTCTGCGA CATGTGCGTT
    ATCCTGTCAG CCAATCAGAA CAATATCGAC GATGCTTCAC TGCAGGACAA GGAATGCATC
    TTGGCATCTC TTAACATCAA ATCTATGCAG TTTGATGACA GCATTGGGGT CTTGCAGGCT
    AATTCCCAAG GTTTCACACC TCCAGGGATG GATAGGTCAT CTCCTGATAA CAGTCCCGTG
    CACGGCTTAG TGCGGCAGGC ATCCGTCACC ACCGGATCAA ACATCCCCAT CATAACTGAA
    CTGGCTAAGC CTGGCAAACT ACTGCCTTTG GTTTCATTCA GTCAGGAAAA AAACAGTGGA
    ACCAACATAC AAATGATAAC AGAACTGGTG AACGACTCCA ACGTTCAGTT TTTGGACCAA
    GACGACGATG ACGACCCAGA CACTGAGCTC TACCTGACCC AGCCGTTCGC CTGTGGTACC
    GCATTCGCCG TCAGCGTGTT GGACTCCCTG ATGAGTGCGA CATACTTCAA TGATAATATC
    CTCACCCTGA TCCGGACGCT GGTCACGGGA GGCGCCACGC CGGAGCTGGA GGCCCTGTTG
    GCTGAGGAGA ACGCTCTGAG AGGTGGCTAC AGCACGCCGC AGACGCTGGC TAACAGGGAC
    AGGTGTCGGG TCGCCCAGCT GGCCCTCTAC GATGGGCCCT TCGCAGACTT GGGGGACGGA
    GGTTGCTATG GAGACTTGTT CTGTAAAGCA TTAAAAACCT ACAACATGCT GTGCTTTGGA
    ATCTATAGAT TGCGAGATGC ACATTTAGGT GCACCAAGCC AGTGCACAAA AAGATATGTC
    ATCACAAACC CGCCTTATGA GTTTGAGATG GTGCCCACGG ACCTGATCTT CTGTCTGATG
    CAGTTCGACC ACAACGCGGG CCAGTCCCGA GCGAGCCTGT CCCACTCCTC CCATTCGTCC
    CATTCCTCCA GCAAGAAGAG CTCGTCTGTT CACTCCATCC CAGCCACCAA CCGGCAGAAC
    CGCAGCAGCA AAGCACGTGA AGCCCGTGAC AAACAGAAAA AAGAGATGGT TTACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54728
    Clone ID Related Accession (Same CDS sequence) XM_017358782.2 , XM_017358782.1
    Accession Version XM_017358782.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3540bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X23
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATGACA TATTTCGAAG ATGAGCATCC GTCAACACTG
    TCACCCAAAA AGAAGCAGCG CAACGGAGGC ATGCGAAATT CACCCAACTG CTCGCCCAAA
    ATGATGAGGC ATGATCCTCT ACTCATTCCA GGAAATGAGC AGATTGAGAG CATGGATGCT
    AATGTCAAAC GATACGACTC TACTGGAATG TTTCACTGGT GTCCATCCAA AGAAATCGAG
    AAGGTCATTC TGACACGCAG CGAGGCCTCA ATGACGGTTT TAAGTGGACA TGTCGTAGTC
    TGCATATTTG GAGATGTCAC GTCAGCTTTA GTGGGGCTTC GAAACTTAGT CATGCCTTTG
    AGAGCAAGCA ACTTCCATTA CCATGAGCTA AAGCCCATTG TGTTTGTGGG CTCGTTAGAT
    TATCTGCGAA GAGAGTGGGA AACCCTTCAC AACTTCCCCA AAGTCTTCAT CTTACCTGGT
    ACGCCATTAA GTCGAGCTGA TTTAAGGGCC GTCAACATCA ACCTCTGCGA CATGTGCGTT
    ATCCTGTCAG CCAATCAGAA CAATATCGAC GATGCTTCAC TGCAGGACAA GGAATGCATC
    TTGGCATCTC TTAACATCAA ATCTATGCAG TTTGATGACA GCATTGGGGT CTTGCAGGCT
    AATTCCCAAG GTTTCACACC TCCAGGGATG GATAGGTCAT CTCCTGATAA CAGTCCCGTG
    CACGGCTTAG TGCGGCAGGC ATCCGTCACC ACCGGATCAA ACATCCCCAT CATAACTGAA
    CTGGCTAAGC CTGGCAAACT ACTGCCTTTG GTTTCATTCA GTCAGGAAAA AAACAGTGGA
    ACCAACATAC AAATGATAAC AGAACTGGTG AACGACTCCA ACGTTCAGTT TTTGGACCAA
    GACGACGATG ACGACCCAGA CACTGAGCTC TACCTGACCC AGCCGTTCGC CTGTGGTACC
    GCATTCGCCG TCAGCGTGTT GGACTCCCTG ATGAGTGCGA CATACTTCAA TGATAATATC
    CTCACCCTGA TCCGGACGCT GGTCACGGGA GGCGCCACGC CGGAGCTGGA GGCCCTGTTG
    GCTGAGGAGA ACGCTCTGAG AGGTGGCTAC AGCACGCCGC AGACGCTGGC TAACAGGGAC
    AGGTGTCGGG TCGCCCAGCT GGCCCTCTAC GATGGGCCCT TCGCAGACTT GGGGGACGGA
    GGTTGCTATG GAGACTTGTT CTGTAAAGCA TTAAAAACCT ACAACATGCT GTGCTTTGGA
    ATCTATAGAT TGCGAGATGC ACATTTAGGT GCACCAAGCC AGTGCACAAA AAGATATGTC
    ATCACAAACC CGCCTTATGA GTTTGAGATG GTGCCCACGG ACCTGATCTT CTGTCTGATG
    CAGTTCGACC ACAACGCGGG CCAGTCCCGA GCGAGCCTGT CCCACTCCTC CCATTCGTCC
    CATTCCTCCA GCAAGAAGAG CTCGTCTGTT CACTCCATCC CAGCCACCAA CCGGCAGAAC
    CGCAGCAGCA AAGCACGTGA AGCCCGTGAC AAACAGAAAA AAGAGATGGT TTACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214271.1
    CDS658..4197
    Translation

    Target ORF information:

    RefSeq Version XM_017358782.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358782.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATGACA TATTTCGAAG ATGAGCATCC GTCAACACTG
    TCACCCAAAA AGAAGCAGCG CAACGGAGGC ATGCGAAATT CACCCAACTG CTCGCCCAAA
    ATGATGAGGC ATGATCCTCT ACTCATTCCA GGAAATGAGC AGATTGAGAG CATGGATGCT
    AATGTCAAAC GATACGACTC TACTGGAATG TTTCACTGGT GTCCATCCAA AGAAATCGAG
    AAGGTCATTC TGACACGCAG CGAGGCCTCA ATGACGGTTT TAAGTGGACA TGTCGTAGTC
    TGCATATTTG GAGATGTCAC GTCAGCTTTA GTGGGGCTTC GAAACTTAGT CATGCCTTTG
    AGAGCAAGCA ACTTCCATTA CCATGAGCTA AAGCCCATTG TGTTTGTGGG CTCGTTAGAT
    TATCTGCGAA GAGAGTGGGA AACCCTTCAC AACTTCCCCA AAGTCTTCAT CTTACCTGGT
    ACGCCATTAA GTCGAGCTGA TTTAAGGGCC GTCAACATCA ACCTCTGCGA CATGTGCGTT
    ATCCTGTCAG CCAATCAGAA CAATATCGAC GATGCTTCAC TGCAGGACAA GGAATGCATC
    TTGGCATCTC TTAACATCAA ATCTATGCAG TTTGATGACA GCATTGGGGT CTTGCAGGCT
    AATTCCCAAG GTTTCACACC TCCAGGGATG GATAGGTCAT CTCCTGATAA CAGTCCCGTG
    CACGGCTTAG TGCGGCAGGC ATCCGTCACC ACCGGATCAA ACATCCCCAT CATAACTGAA
    CTGGCTAAGC CTGGCAAACT ACTGCCTTTG GTTTCATTCA GTCAGGAAAA AAACAGTGGA
    ACCAACATAC AAATGATAAC AGAACTGGTG AACGACTCCA ACGTTCAGTT TTTGGACCAA
    GACGACGATG ACGACCCAGA CACTGAGCTC TACCTGACCC AGCCGTTCGC CTGTGGTACC
    GCATTCGCCG TCAGCGTGTT GGACTCCCTG ATGAGTGCGA CATACTTCAA TGATAATATC
    CTCACCCTGA TCCGGACGCT GGTCACGGGA GGCGCCACGC CGGAGCTGGA GGCCCTGTTG
    GCTGAGGAGA ACGCTCTGAG AGGTGGCTAC AGCACGCCGC AGACGCTGGC TAACAGGGAC
    AGGTGTCGGG TCGCCCAGCT GGCCCTCTAC GATGGGCCCT TCGCAGACTT GGGGGACGGA
    GGTTGCTATG GAGACTTGTT CTGTAAAGCA TTAAAAACCT ACAACATGCT GTGCTTTGGA
    ATCTATAGAT TGCGAGATGC ACATTTAGGT GCACCAAGCC AGTGCACAAA AAGATATGTC
    ATCACAAACC CGCCTTATGA GTTTGAGATG GTGCCCACGG ACCTGATCTT CTGTCTGATG
    CAGTTCGACC ACAACGCGGG CCAGTCCCGA GCGAGCCTGT CCCACTCCTC CCATTCGTCC
    CATTCCTCCA GCAAGAAGAG CTCGTCTGTT CACTCCATCC CAGCCACCAA CCGGCAGAAC
    CGCAGCAGCA AAGCACGTGA AGCCCGTGAC AAACAGAAAA AAGAGATGGT TTACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54729
    Clone ID Related Accession (Same CDS sequence) XM_017358783.2 , XM_017358783.1
    Accession Version XM_017358783.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3459bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X30
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358783.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATCG AAGATGAGCA TCCGTCAACA
    CTGTCACCCA AAAAGAAGCA GCGCAACGGA GGCATGCGAA ATTCACCCAA CTGCTCGCCC
    AAAATGATGA GGCATGATCC TCTACTCATT CCAGGAAATG AGCAGATTGA GAGCATGGAT
    GCTAATGTCA AACGATACGA CTCTACTGGA ATGTTTCACT GGTGTCCATC CAAAGAAATC
    GAGAAGGTCA TTCTGACACG CAGCGAGGCC TCAATGACGG TTTTAAGTGG ACATGTCGTA
    GTCTGCATAT TTGGAGATGT CACGTCAGCT TTAGTGGGGC TTCGAAACTT AGTCATGCCT
    TTGAGAGCAA GCAACTTCCA TTACCATGAG CTAAAGCCCA TTGTGTTTGT GGGCTCGTTA
    GATTATCTGC GAAGAGAGTG GGAAACCCTT CACAACTTCC CCAAAGTCTT CATCTTACCT
    GGTACGCCAT TAAGTCGAGC TGATTTAAGG GCCGTCAACA TCAACCTCTG CGACATGTGC
    GTTATCCTGT CAGCCAATCA GAACAATATC GACGATGCTT CACTGCAGGA CAAGGAATGC
    ATCTTGGCAT CTCTTAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG
    GCTAATTCCC AAGGTTTCAC ACCTCCAGGG ATGGATAGGT CATCTCCTGA TAACAGTCCC
    GTGCACGGCT TAGTGCGGCA GGCATCCGTC ACCACCGGAT CAAACATCCC CATCATAACT
    GAACTGGTGA ACGACTCCAA CGTTCAGTTT TTGGACCAAG ACGACGATGA CGACCCAGAC
    ACTGAGCTCT ACCTGACCCA GCCGTTCGCC TGTGGTACCG CATTCGCCGT CAGCGTGTTG
    GACTCCCTGA TGAGTGCGAC ATACTTCAAT GATAATATCC TCACCCTGAT CCGGACGCTG
    GTCACGGGAG GCGCCACGCC GGAGCTGGAG GCCCTGTTGG CTGAGGAGAA CGCTCTGAGA
    GGTGGCTACA GCACGCCGCA GACGCTGGCT AACAGGGACA GGTGTCGGGT CGCCCAGCTG
    GCCCTCTACG ATGGGCCCTT CGCAGACTTG GGGGACGGAG GTTGCTATGG AGACTTGTTC
    TGTAAAGCAT TAAAAACCTA CAACATGCTG TGCTTTGGAA TCTATAGATT GCGAGATGCA
    CATTTAGGTG CACCAAGCCA GTGCACAAAA AGATATGTCA TCACAAACCC GCCTTATGAG
    TTTGAGATGG TGCCCACGGA CCTGATCTTC TGTCTGATGC AGTTCGACCA CAACGCGGGC
    CAGTCCCGAG CGAGCCTGTC CCACTCCTCC CATTCGTCCC ATTCCTCCAG CAAGAAGAGC
    TCGTCTGTTC ACTCCATCCC AGCCACCAAC CGGCAGAACC GCAGCAGCAA AGCACGTGAA
    GCCCGTGACA AACAGAAAAA AGAGATGGTT TACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214272.1
    CDS675..4133
    Translation

    Target ORF information:

    RefSeq Version XM_017358783.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X30, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358783.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATCG AAGATGAGCA TCCGTCAACA
    CTGTCACCCA AAAAGAAGCA GCGCAACGGA GGCATGCGAA ATTCACCCAA CTGCTCGCCC
    AAAATGATGA GGCATGATCC TCTACTCATT CCAGGAAATG AGCAGATTGA GAGCATGGAT
    GCTAATGTCA AACGATACGA CTCTACTGGA ATGTTTCACT GGTGTCCATC CAAAGAAATC
    GAGAAGGTCA TTCTGACACG CAGCGAGGCC TCAATGACGG TTTTAAGTGG ACATGTCGTA
    GTCTGCATAT TTGGAGATGT CACGTCAGCT TTAGTGGGGC TTCGAAACTT AGTCATGCCT
    TTGAGAGCAA GCAACTTCCA TTACCATGAG CTAAAGCCCA TTGTGTTTGT GGGCTCGTTA
    GATTATCTGC GAAGAGAGTG GGAAACCCTT CACAACTTCC CCAAAGTCTT CATCTTACCT
    GGTACGCCAT TAAGTCGAGC TGATTTAAGG GCCGTCAACA TCAACCTCTG CGACATGTGC
    GTTATCCTGT CAGCCAATCA GAACAATATC GACGATGCTT CACTGCAGGA CAAGGAATGC
    ATCTTGGCAT CTCTTAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG
    GCTAATTCCC AAGGTTTCAC ACCTCCAGGG ATGGATAGGT CATCTCCTGA TAACAGTCCC
    GTGCACGGCT TAGTGCGGCA GGCATCCGTC ACCACCGGAT CAAACATCCC CATCATAACT
    GAACTGGTGA ACGACTCCAA CGTTCAGTTT TTGGACCAAG ACGACGATGA CGACCCAGAC
    ACTGAGCTCT ACCTGACCCA GCCGTTCGCC TGTGGTACCG CATTCGCCGT CAGCGTGTTG
    GACTCCCTGA TGAGTGCGAC ATACTTCAAT GATAATATCC TCACCCTGAT CCGGACGCTG
    GTCACGGGAG GCGCCACGCC GGAGCTGGAG GCCCTGTTGG CTGAGGAGAA CGCTCTGAGA
    GGTGGCTACA GCACGCCGCA GACGCTGGCT AACAGGGACA GGTGTCGGGT CGCCCAGCTG
    GCCCTCTACG ATGGGCCCTT CGCAGACTTG GGGGACGGAG GTTGCTATGG AGACTTGTTC
    TGTAAAGCAT TAAAAACCTA CAACATGCTG TGCTTTGGAA TCTATAGATT GCGAGATGCA
    CATTTAGGTG CACCAAGCCA GTGCACAAAA AGATATGTCA TCACAAACCC GCCTTATGAG
    TTTGAGATGG TGCCCACGGA CCTGATCTTC TGTCTGATGC AGTTCGACCA CAACGCGGGC
    CAGTCCCGAG CGAGCCTGTC CCACTCCTCC CATTCGTCCC ATTCCTCCAG CAAGAAGAGC
    TCGTCTGTTC ACTCCATCCC AGCCACCAAC CGGCAGAACC GCAGCAGCAA AGCACGTGAA
    GCCCGTGACA AACAGAAAAA AGAGATGGTT TACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54729
    Clone ID Related Accession (Same CDS sequence) XM_017358783.2 , XM_017358783.1
    Accession Version XM_017358783.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3459bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X24
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATCG AAGATGAGCA TCCGTCAACA
    CTGTCACCCA AAAAGAAGCA GCGCAACGGA GGCATGCGAA ATTCACCCAA CTGCTCGCCC
    AAAATGATGA GGCATGATCC TCTACTCATT CCAGGAAATG AGCAGATTGA GAGCATGGAT
    GCTAATGTCA AACGATACGA CTCTACTGGA ATGTTTCACT GGTGTCCATC CAAAGAAATC
    GAGAAGGTCA TTCTGACACG CAGCGAGGCC TCAATGACGG TTTTAAGTGG ACATGTCGTA
    GTCTGCATAT TTGGAGATGT CACGTCAGCT TTAGTGGGGC TTCGAAACTT AGTCATGCCT
    TTGAGAGCAA GCAACTTCCA TTACCATGAG CTAAAGCCCA TTGTGTTTGT GGGCTCGTTA
    GATTATCTGC GAAGAGAGTG GGAAACCCTT CACAACTTCC CCAAAGTCTT CATCTTACCT
    GGTACGCCAT TAAGTCGAGC TGATTTAAGG GCCGTCAACA TCAACCTCTG CGACATGTGC
    GTTATCCTGT CAGCCAATCA GAACAATATC GACGATGCTT CACTGCAGGA CAAGGAATGC
    ATCTTGGCAT CTCTTAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG
    GCTAATTCCC AAGGTTTCAC ACCTCCAGGG ATGGATAGGT CATCTCCTGA TAACAGTCCC
    GTGCACGGCT TAGTGCGGCA GGCATCCGTC ACCACCGGAT CAAACATCCC CATCATAACT
    GAACTGGTGA ACGACTCCAA CGTTCAGTTT TTGGACCAAG ACGACGATGA CGACCCAGAC
    ACTGAGCTCT ACCTGACCCA GCCGTTCGCC TGTGGTACCG CATTCGCCGT CAGCGTGTTG
    GACTCCCTGA TGAGTGCGAC ATACTTCAAT GATAATATCC TCACCCTGAT CCGGACGCTG
    GTCACGGGAG GCGCCACGCC GGAGCTGGAG GCCCTGTTGG CTGAGGAGAA CGCTCTGAGA
    GGTGGCTACA GCACGCCGCA GACGCTGGCT AACAGGGACA GGTGTCGGGT CGCCCAGCTG
    GCCCTCTACG ATGGGCCCTT CGCAGACTTG GGGGACGGAG GTTGCTATGG AGACTTGTTC
    TGTAAAGCAT TAAAAACCTA CAACATGCTG TGCTTTGGAA TCTATAGATT GCGAGATGCA
    CATTTAGGTG CACCAAGCCA GTGCACAAAA AGATATGTCA TCACAAACCC GCCTTATGAG
    TTTGAGATGG TGCCCACGGA CCTGATCTTC TGTCTGATGC AGTTCGACCA CAACGCGGGC
    CAGTCCCGAG CGAGCCTGTC CCACTCCTCC CATTCGTCCC ATTCCTCCAG CAAGAAGAGC
    TCGTCTGTTC ACTCCATCCC AGCCACCAAC CGGCAGAACC GCAGCAGCAA AGCACGTGAA
    GCCCGTGACA AACAGAAAAA AGAGATGGTT TACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214272.1
    CDS656..4114
    Translation

    Target ORF information:

    RefSeq Version XM_017358783.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X24, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358783.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATCG AAGATGAGCA TCCGTCAACA
    CTGTCACCCA AAAAGAAGCA GCGCAACGGA GGCATGCGAA ATTCACCCAA CTGCTCGCCC
    AAAATGATGA GGCATGATCC TCTACTCATT CCAGGAAATG AGCAGATTGA GAGCATGGAT
    GCTAATGTCA AACGATACGA CTCTACTGGA ATGTTTCACT GGTGTCCATC CAAAGAAATC
    GAGAAGGTCA TTCTGACACG CAGCGAGGCC TCAATGACGG TTTTAAGTGG ACATGTCGTA
    GTCTGCATAT TTGGAGATGT CACGTCAGCT TTAGTGGGGC TTCGAAACTT AGTCATGCCT
    TTGAGAGCAA GCAACTTCCA TTACCATGAG CTAAAGCCCA TTGTGTTTGT GGGCTCGTTA
    GATTATCTGC GAAGAGAGTG GGAAACCCTT CACAACTTCC CCAAAGTCTT CATCTTACCT
    GGTACGCCAT TAAGTCGAGC TGATTTAAGG GCCGTCAACA TCAACCTCTG CGACATGTGC
    GTTATCCTGT CAGCCAATCA GAACAATATC GACGATGCTT CACTGCAGGA CAAGGAATGC
    ATCTTGGCAT CTCTTAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG
    GCTAATTCCC AAGGTTTCAC ACCTCCAGGG ATGGATAGGT CATCTCCTGA TAACAGTCCC
    GTGCACGGCT TAGTGCGGCA GGCATCCGTC ACCACCGGAT CAAACATCCC CATCATAACT
    GAACTGGTGA ACGACTCCAA CGTTCAGTTT TTGGACCAAG ACGACGATGA CGACCCAGAC
    ACTGAGCTCT ACCTGACCCA GCCGTTCGCC TGTGGTACCG CATTCGCCGT CAGCGTGTTG
    GACTCCCTGA TGAGTGCGAC ATACTTCAAT GATAATATCC TCACCCTGAT CCGGACGCTG
    GTCACGGGAG GCGCCACGCC GGAGCTGGAG GCCCTGTTGG CTGAGGAGAA CGCTCTGAGA
    GGTGGCTACA GCACGCCGCA GACGCTGGCT AACAGGGACA GGTGTCGGGT CGCCCAGCTG
    GCCCTCTACG ATGGGCCCTT CGCAGACTTG GGGGACGGAG GTTGCTATGG AGACTTGTTC
    TGTAAAGCAT TAAAAACCTA CAACATGCTG TGCTTTGGAA TCTATAGATT GCGAGATGCA
    CATTTAGGTG CACCAAGCCA GTGCACAAAA AGATATGTCA TCACAAACCC GCCTTATGAG
    TTTGAGATGG TGCCCACGGA CCTGATCTTC TGTCTGATGC AGTTCGACCA CAACGCGGGC
    CAGTCCCGAG CGAGCCTGTC CCACTCCTCC CATTCGTCCC ATTCCTCCAG CAAGAAGAGC
    TCGTCTGTTC ACTCCATCCC AGCCACCAAC CGGCAGAACC GCAGCAGCAA AGCACGTGAA
    GCCCGTGACA AACAGAAAAA AGAGATGGTT TACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54730
    Clone ID Related Accession (Same CDS sequence) XM_017358784.1 , XM_017358784.2
    Accession Version XM_017358784.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X25
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATGACA TATTTCGAAG ATGAGCATCC GTCAACACTG
    TCACCCAAAA AGAAGCAGCG CAACGGAGGC ATGCGAAATT CACCCAACTG CTCGCCCAAA
    ATGATGAGGC ATGATCCTCT ACTCATTCCA GGAAATGAGC AGATTGAGAG CATGGATGCT
    AATGTCAAAC GATACGACTC TACTGGAATG TTTCACTGGT GTCCATCCAA AGAAATCGAG
    AAGGTCATTC TGACACGCAG CGAGGCCTCA ATGACGGTTT TAAGTGGACA TGTCGTAGTC
    TGCATATTTG GAGATGTCAC GTCAGCTTTA GTGGGGCTTC GAAACTTAGT CATGCCTTTG
    AGAGCAAGCA ACTTCCATTA CCATGAGCTA AAGCCCATTG TGTTTGTGGG CTCGTTAGAT
    TATCTGCGAA GAGAGTGGGA AACCCTTCAC AACTTCCCCA AAGTCTTCAT CTTACCTGGT
    ACGCCATTAA GTCGAGCTGA TTTAAGGGCC GTCAACATCA ACCTCTGCGA CATGTGCGTT
    ATCCTGTCAG CCAATCAGAA CAATATCGAC GATGCTTCAC TGCAGGACAA GGAATGCATC
    TTGGCATCTC TTAACATCAA ATCTATGCAG TTTGATGACA GCATTGGGGT CTTGCAGGCT
    AATTCCCAAG GTTTCACACC TCCAGGGATG GATAGGTCAT CTCCTGATAA CAGTCCCGTG
    CACGGCTTAG TGCGGCAGGC ATCCGTCACC ACCGGATCAA ACATCCCCAT CATAACTGAA
    CTGGTGAACG ACTCCAACGT TCAGTTTTTG GACCAAGACG ACGATGACGA CCCAGACACT
    GAGCTCTACC TGACCCAGCC GTTCGCCTGT GGTACCGCAT TCGCCGTCAG CGTGTTGGAC
    TCCCTGATGA GTGCGACATA CTTCAATGAT AATATCCTCA CCCTGATCCG GACGCTGGTC
    ACGGGAGGCG CCACGCCGGA GCTGGAGGCC CTGTTGGCTG AGGAGAACGC TCTGAGAGGT
    GGCTACAGCA CGCCGCAGAC GCTGGCTAAC AGGGACAGGT GTCGGGTCGC CCAGCTGGCC
    CTCTACGATG GGCCCTTCGC AGACTTGGGG GACGGAGGTT GCTATGGAGA CTTGTTCTGT
    AAAGCATTAA AAACCTACAA CATGCTGTGC TTTGGAATCT ATAGATTGCG AGATGCACAT
    TTAGGTGCAC CAAGCCAGTG CACAAAAAGA TATGTCATCA CAAACCCGCC TTATGAGTTT
    GAGATGGTGC CCACGGACCT GATCTTCTGT CTGATGCAGT TCGACCACAA CGCGGGCCAG
    TCCCGAGCGA GCCTGTCCCA CTCCTCCCAT TCGTCCCATT CCTCCAGCAA GAAGAGCTCG
    TCTGTTCACT CCATCCCAGC CACCAACCGG CAGAACCGCA GCAGCAAAGC ACGTGAAGCC
    CGTGACAAAC AGAAAAAAGA GATGGTTTAC AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214273.1
    CDS654..4109
    Translation

    Target ORF information:

    RefSeq Version XM_017358784.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358784.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATGACA TATTTCGAAG ATGAGCATCC GTCAACACTG
    TCACCCAAAA AGAAGCAGCG CAACGGAGGC ATGCGAAATT CACCCAACTG CTCGCCCAAA
    ATGATGAGGC ATGATCCTCT ACTCATTCCA GGAAATGAGC AGATTGAGAG CATGGATGCT
    AATGTCAAAC GATACGACTC TACTGGAATG TTTCACTGGT GTCCATCCAA AGAAATCGAG
    AAGGTCATTC TGACACGCAG CGAGGCCTCA ATGACGGTTT TAAGTGGACA TGTCGTAGTC
    TGCATATTTG GAGATGTCAC GTCAGCTTTA GTGGGGCTTC GAAACTTAGT CATGCCTTTG
    AGAGCAAGCA ACTTCCATTA CCATGAGCTA AAGCCCATTG TGTTTGTGGG CTCGTTAGAT
    TATCTGCGAA GAGAGTGGGA AACCCTTCAC AACTTCCCCA AAGTCTTCAT CTTACCTGGT
    ACGCCATTAA GTCGAGCTGA TTTAAGGGCC GTCAACATCA ACCTCTGCGA CATGTGCGTT
    ATCCTGTCAG CCAATCAGAA CAATATCGAC GATGCTTCAC TGCAGGACAA GGAATGCATC
    TTGGCATCTC TTAACATCAA ATCTATGCAG TTTGATGACA GCATTGGGGT CTTGCAGGCT
    AATTCCCAAG GTTTCACACC TCCAGGGATG GATAGGTCAT CTCCTGATAA CAGTCCCGTG
    CACGGCTTAG TGCGGCAGGC ATCCGTCACC ACCGGATCAA ACATCCCCAT CATAACTGAA
    CTGGTGAACG ACTCCAACGT TCAGTTTTTG GACCAAGACG ACGATGACGA CCCAGACACT
    GAGCTCTACC TGACCCAGCC GTTCGCCTGT GGTACCGCAT TCGCCGTCAG CGTGTTGGAC
    TCCCTGATGA GTGCGACATA CTTCAATGAT AATATCCTCA CCCTGATCCG GACGCTGGTC
    ACGGGAGGCG CCACGCCGGA GCTGGAGGCC CTGTTGGCTG AGGAGAACGC TCTGAGAGGT
    GGCTACAGCA CGCCGCAGAC GCTGGCTAAC AGGGACAGGT GTCGGGTCGC CCAGCTGGCC
    CTCTACGATG GGCCCTTCGC AGACTTGGGG GACGGAGGTT GCTATGGAGA CTTGTTCTGT
    AAAGCATTAA AAACCTACAA CATGCTGTGC TTTGGAATCT ATAGATTGCG AGATGCACAT
    TTAGGTGCAC CAAGCCAGTG CACAAAAAGA TATGTCATCA CAAACCCGCC TTATGAGTTT
    GAGATGGTGC CCACGGACCT GATCTTCTGT CTGATGCAGT TCGACCACAA CGCGGGCCAG
    TCCCGAGCGA GCCTGTCCCA CTCCTCCCAT TCGTCCCATT CCTCCAGCAA GAAGAGCTCG
    TCTGTTCACT CCATCCCAGC CACCAACCGG CAGAACCGCA GCAGCAAAGC ACGTGAAGCC
    CGTGACAAAC AGAAAAAAGA GATGGTTTAC AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54730
    Clone ID Related Accession (Same CDS sequence) XM_017358784.1 , XM_017358784.2
    Accession Version XM_017358784.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X31
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358784.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATGACA TATTTCGAAG ATGAGCATCC GTCAACACTG
    TCACCCAAAA AGAAGCAGCG CAACGGAGGC ATGCGAAATT CACCCAACTG CTCGCCCAAA
    ATGATGAGGC ATGATCCTCT ACTCATTCCA GGAAATGAGC AGATTGAGAG CATGGATGCT
    AATGTCAAAC GATACGACTC TACTGGAATG TTTCACTGGT GTCCATCCAA AGAAATCGAG
    AAGGTCATTC TGACACGCAG CGAGGCCTCA ATGACGGTTT TAAGTGGACA TGTCGTAGTC
    TGCATATTTG GAGATGTCAC GTCAGCTTTA GTGGGGCTTC GAAACTTAGT CATGCCTTTG
    AGAGCAAGCA ACTTCCATTA CCATGAGCTA AAGCCCATTG TGTTTGTGGG CTCGTTAGAT
    TATCTGCGAA GAGAGTGGGA AACCCTTCAC AACTTCCCCA AAGTCTTCAT CTTACCTGGT
    ACGCCATTAA GTCGAGCTGA TTTAAGGGCC GTCAACATCA ACCTCTGCGA CATGTGCGTT
    ATCCTGTCAG CCAATCAGAA CAATATCGAC GATGCTTCAC TGCAGGACAA GGAATGCATC
    TTGGCATCTC TTAACATCAA ATCTATGCAG TTTGATGACA GCATTGGGGT CTTGCAGGCT
    AATTCCCAAG GTTTCACACC TCCAGGGATG GATAGGTCAT CTCCTGATAA CAGTCCCGTG
    CACGGCTTAG TGCGGCAGGC ATCCGTCACC ACCGGATCAA ACATCCCCAT CATAACTGAA
    CTGGTGAACG ACTCCAACGT TCAGTTTTTG GACCAAGACG ACGATGACGA CCCAGACACT
    GAGCTCTACC TGACCCAGCC GTTCGCCTGT GGTACCGCAT TCGCCGTCAG CGTGTTGGAC
    TCCCTGATGA GTGCGACATA CTTCAATGAT AATATCCTCA CCCTGATCCG GACGCTGGTC
    ACGGGAGGCG CCACGCCGGA GCTGGAGGCC CTGTTGGCTG AGGAGAACGC TCTGAGAGGT
    GGCTACAGCA CGCCGCAGAC GCTGGCTAAC AGGGACAGGT GTCGGGTCGC CCAGCTGGCC
    CTCTACGATG GGCCCTTCGC AGACTTGGGG GACGGAGGTT GCTATGGAGA CTTGTTCTGT
    AAAGCATTAA AAACCTACAA CATGCTGTGC TTTGGAATCT ATAGATTGCG AGATGCACAT
    TTAGGTGCAC CAAGCCAGTG CACAAAAAGA TATGTCATCA CAAACCCGCC TTATGAGTTT
    GAGATGGTGC CCACGGACCT GATCTTCTGT CTGATGCAGT TCGACCACAA CGCGGGCCAG
    TCCCGAGCGA GCCTGTCCCA CTCCTCCCAT TCGTCCCATT CCTCCAGCAA GAAGAGCTCG
    TCTGTTCACT CCATCCCAGC CACCAACCGG CAGAACCGCA GCAGCAAAGC ACGTGAAGCC
    CGTGACAAAC AGAAAAAAGA GATGGTTTAC AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214273.1
    CDS675..4130
    Translation

    Target ORF information:

    RefSeq Version XM_017358784.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X31, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358784.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATGACA TATTTCGAAG ATGAGCATCC GTCAACACTG
    TCACCCAAAA AGAAGCAGCG CAACGGAGGC ATGCGAAATT CACCCAACTG CTCGCCCAAA
    ATGATGAGGC ATGATCCTCT ACTCATTCCA GGAAATGAGC AGATTGAGAG CATGGATGCT
    AATGTCAAAC GATACGACTC TACTGGAATG TTTCACTGGT GTCCATCCAA AGAAATCGAG
    AAGGTCATTC TGACACGCAG CGAGGCCTCA ATGACGGTTT TAAGTGGACA TGTCGTAGTC
    TGCATATTTG GAGATGTCAC GTCAGCTTTA GTGGGGCTTC GAAACTTAGT CATGCCTTTG
    AGAGCAAGCA ACTTCCATTA CCATGAGCTA AAGCCCATTG TGTTTGTGGG CTCGTTAGAT
    TATCTGCGAA GAGAGTGGGA AACCCTTCAC AACTTCCCCA AAGTCTTCAT CTTACCTGGT
    ACGCCATTAA GTCGAGCTGA TTTAAGGGCC GTCAACATCA ACCTCTGCGA CATGTGCGTT
    ATCCTGTCAG CCAATCAGAA CAATATCGAC GATGCTTCAC TGCAGGACAA GGAATGCATC
    TTGGCATCTC TTAACATCAA ATCTATGCAG TTTGATGACA GCATTGGGGT CTTGCAGGCT
    AATTCCCAAG GTTTCACACC TCCAGGGATG GATAGGTCAT CTCCTGATAA CAGTCCCGTG
    CACGGCTTAG TGCGGCAGGC ATCCGTCACC ACCGGATCAA ACATCCCCAT CATAACTGAA
    CTGGTGAACG ACTCCAACGT TCAGTTTTTG GACCAAGACG ACGATGACGA CCCAGACACT
    GAGCTCTACC TGACCCAGCC GTTCGCCTGT GGTACCGCAT TCGCCGTCAG CGTGTTGGAC
    TCCCTGATGA GTGCGACATA CTTCAATGAT AATATCCTCA CCCTGATCCG GACGCTGGTC
    ACGGGAGGCG CCACGCCGGA GCTGGAGGCC CTGTTGGCTG AGGAGAACGC TCTGAGAGGT
    GGCTACAGCA CGCCGCAGAC GCTGGCTAAC AGGGACAGGT GTCGGGTCGC CCAGCTGGCC
    CTCTACGATG GGCCCTTCGC AGACTTGGGG GACGGAGGTT GCTATGGAGA CTTGTTCTGT
    AAAGCATTAA AAACCTACAA CATGCTGTGC TTTGGAATCT ATAGATTGCG AGATGCACAT
    TTAGGTGCAC CAAGCCAGTG CACAAAAAGA TATGTCATCA CAAACCCGCC TTATGAGTTT
    GAGATGGTGC CCACGGACCT GATCTTCTGT CTGATGCAGT TCGACCACAA CGCGGGCCAG
    TCCCGAGCGA GCCTGTCCCA CTCCTCCCAT TCGTCCCATT CCTCCAGCAA GAAGAGCTCG
    TCTGTTCACT CCATCCCAGC CACCAACCGG CAGAACCGCA GCAGCAAAGC ACGTGAAGCC
    CGTGACAAAC AGAAAAAAGA GATGGTTTAC AGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54731
    Clone ID Related Accession (Same CDS sequence) XM_017358785.2 , XM_017358785.1
    Accession Version XM_017358785.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3447bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X32
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358785.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATCGAA GATGAGCATC CGTCAACACT GTCACCCAAA
    AAGAAGCAGC GCAACGGAGG CATGCGAAAT TCACCCAACT GCTCGCCCAA AATGATGAGG
    CATGATCCTC TACTCATTCC AGGAAATGAG CAGATTGAGA GCATGGATGC TAATGTCAAA
    CGATACGACT CTACTGGAAT GTTTCACTGG TGTCCATCCA AAGAAATCGA GAAGGTCATT
    CTGACACGCA GCGAGGCCTC AATGACGGTT TTAAGTGGAC ATGTCGTAGT CTGCATATTT
    GGAGATGTCA CGTCAGCTTT AGTGGGGCTT CGAAACTTAG TCATGCCTTT GAGAGCAAGC
    AACTTCCATT ACCATGAGCT AAAGCCCATT GTGTTTGTGG GCTCGTTAGA TTATCTGCGA
    AGAGAGTGGG AAACCCTTCA CAACTTCCCC AAAGTCTTCA TCTTACCTGG TACGCCATTA
    AGTCGAGCTG ATTTAAGGGC CGTCAACATC AACCTCTGCG ACATGTGCGT TATCCTGTCA
    GCCAATCAGA ACAATATCGA CGATGCTTCA CTGCAGGACA AGGAATGCAT CTTGGCATCT
    CTTAACATCA AATCTATGCA GTTTGATGAC AGCATTGGGG TCTTGCAGGC TAATTCCCAA
    GGTTTCACAC CTCCAGGGAT GGATAGGTCA TCTCCTGATA ACAGTCCCGT GCACGGCTTA
    GTGCGGCAGG CATCCGTCAC CACCGGATCA AACATCCCCA TCATAACTGA ACTGGTGAAC
    GACTCCAACG TTCAGTTTTT GGACCAAGAC GACGATGACG ACCCAGACAC TGAGCTCTAC
    CTGACCCAGC CGTTCGCCTG TGGTACCGCA TTCGCCGTCA GCGTGTTGGA CTCCCTGATG
    AGTGCGACAT ACTTCAATGA TAATATCCTC ACCCTGATCC GGACGCTGGT CACGGGAGGC
    GCCACGCCGG AGCTGGAGGC CCTGTTGGCT GAGGAGAACG CTCTGAGAGG TGGCTACAGC
    ACGCCGCAGA CGCTGGCTAA CAGGGACAGG TGTCGGGTCG CCCAGCTGGC CCTCTACGAT
    GGGCCCTTCG CAGACTTGGG GGACGGAGGT TGCTATGGAG ACTTGTTCTG TAAAGCATTA
    AAAACCTACA ACATGCTGTG CTTTGGAATC TATAGATTGC GAGATGCACA TTTAGGTGCA
    CCAAGCCAGT GCACAAAAAG ATATGTCATC ACAAACCCGC CTTATGAGTT TGAGATGGTG
    CCCACGGACC TGATCTTCTG TCTGATGCAG TTCGACCACA ACGCGGGCCA GTCCCGAGCG
    AGCCTGTCCC ACTCCTCCCA TTCGTCCCAT TCCTCCAGCA AGAAGAGCTC GTCTGTTCAC
    TCCATCCCAG CCACCAACCG GCAGAACCGC AGCAGCAAAG CACGTGAAGC CCGTGACAAA
    CAGAAAAAAG AGATGGTTTA CAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214274.1
    CDS674..4120
    Translation

    Target ORF information:

    RefSeq Version XM_017358785.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X32, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358785.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATCGAA GATGAGCATC CGTCAACACT GTCACCCAAA
    AAGAAGCAGC GCAACGGAGG CATGCGAAAT TCACCCAACT GCTCGCCCAA AATGATGAGG
    CATGATCCTC TACTCATTCC AGGAAATGAG CAGATTGAGA GCATGGATGC TAATGTCAAA
    CGATACGACT CTACTGGAAT GTTTCACTGG TGTCCATCCA AAGAAATCGA GAAGGTCATT
    CTGACACGCA GCGAGGCCTC AATGACGGTT TTAAGTGGAC ATGTCGTAGT CTGCATATTT
    GGAGATGTCA CGTCAGCTTT AGTGGGGCTT CGAAACTTAG TCATGCCTTT GAGAGCAAGC
    AACTTCCATT ACCATGAGCT AAAGCCCATT GTGTTTGTGG GCTCGTTAGA TTATCTGCGA
    AGAGAGTGGG AAACCCTTCA CAACTTCCCC AAAGTCTTCA TCTTACCTGG TACGCCATTA
    AGTCGAGCTG ATTTAAGGGC CGTCAACATC AACCTCTGCG ACATGTGCGT TATCCTGTCA
    GCCAATCAGA ACAATATCGA CGATGCTTCA CTGCAGGACA AGGAATGCAT CTTGGCATCT
    CTTAACATCA AATCTATGCA GTTTGATGAC AGCATTGGGG TCTTGCAGGC TAATTCCCAA
    GGTTTCACAC CTCCAGGGAT GGATAGGTCA TCTCCTGATA ACAGTCCCGT GCACGGCTTA
    GTGCGGCAGG CATCCGTCAC CACCGGATCA AACATCCCCA TCATAACTGA ACTGGTGAAC
    GACTCCAACG TTCAGTTTTT GGACCAAGAC GACGATGACG ACCCAGACAC TGAGCTCTAC
    CTGACCCAGC CGTTCGCCTG TGGTACCGCA TTCGCCGTCA GCGTGTTGGA CTCCCTGATG
    AGTGCGACAT ACTTCAATGA TAATATCCTC ACCCTGATCC GGACGCTGGT CACGGGAGGC
    GCCACGCCGG AGCTGGAGGC CCTGTTGGCT GAGGAGAACG CTCTGAGAGG TGGCTACAGC
    ACGCCGCAGA CGCTGGCTAA CAGGGACAGG TGTCGGGTCG CCCAGCTGGC CCTCTACGAT
    GGGCCCTTCG CAGACTTGGG GGACGGAGGT TGCTATGGAG ACTTGTTCTG TAAAGCATTA
    AAAACCTACA ACATGCTGTG CTTTGGAATC TATAGATTGC GAGATGCACA TTTAGGTGCA
    CCAAGCCAGT GCACAAAAAG ATATGTCATC ACAAACCCGC CTTATGAGTT TGAGATGGTG
    CCCACGGACC TGATCTTCTG TCTGATGCAG TTCGACCACA ACGCGGGCCA GTCCCGAGCG
    AGCCTGTCCC ACTCCTCCCA TTCGTCCCAT TCCTCCAGCA AGAAGAGCTC GTCTGTTCAC
    TCCATCCCAG CCACCAACCG GCAGAACCGC AGCAGCAAAG CACGTGAAGC CCGTGACAAA
    CAGAAAAAAG AGATGGTTTA CAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54731
    Clone ID Related Accession (Same CDS sequence) XM_017358785.2 , XM_017358785.1
    Accession Version XM_017358785.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3447bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X26
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATCGAA GATGAGCATC CGTCAACACT GTCACCCAAA
    AAGAAGCAGC GCAACGGAGG CATGCGAAAT TCACCCAACT GCTCGCCCAA AATGATGAGG
    CATGATCCTC TACTCATTCC AGGAAATGAG CAGATTGAGA GCATGGATGC TAATGTCAAA
    CGATACGACT CTACTGGAAT GTTTCACTGG TGTCCATCCA AAGAAATCGA GAAGGTCATT
    CTGACACGCA GCGAGGCCTC AATGACGGTT TTAAGTGGAC ATGTCGTAGT CTGCATATTT
    GGAGATGTCA CGTCAGCTTT AGTGGGGCTT CGAAACTTAG TCATGCCTTT GAGAGCAAGC
    AACTTCCATT ACCATGAGCT AAAGCCCATT GTGTTTGTGG GCTCGTTAGA TTATCTGCGA
    AGAGAGTGGG AAACCCTTCA CAACTTCCCC AAAGTCTTCA TCTTACCTGG TACGCCATTA
    AGTCGAGCTG ATTTAAGGGC CGTCAACATC AACCTCTGCG ACATGTGCGT TATCCTGTCA
    GCCAATCAGA ACAATATCGA CGATGCTTCA CTGCAGGACA AGGAATGCAT CTTGGCATCT
    CTTAACATCA AATCTATGCA GTTTGATGAC AGCATTGGGG TCTTGCAGGC TAATTCCCAA
    GGTTTCACAC CTCCAGGGAT GGATAGGTCA TCTCCTGATA ACAGTCCCGT GCACGGCTTA
    GTGCGGCAGG CATCCGTCAC CACCGGATCA AACATCCCCA TCATAACTGA ACTGGTGAAC
    GACTCCAACG TTCAGTTTTT GGACCAAGAC GACGATGACG ACCCAGACAC TGAGCTCTAC
    CTGACCCAGC CGTTCGCCTG TGGTACCGCA TTCGCCGTCA GCGTGTTGGA CTCCCTGATG
    AGTGCGACAT ACTTCAATGA TAATATCCTC ACCCTGATCC GGACGCTGGT CACGGGAGGC
    GCCACGCCGG AGCTGGAGGC CCTGTTGGCT GAGGAGAACG CTCTGAGAGG TGGCTACAGC
    ACGCCGCAGA CGCTGGCTAA CAGGGACAGG TGTCGGGTCG CCCAGCTGGC CCTCTACGAT
    GGGCCCTTCG CAGACTTGGG GGACGGAGGT TGCTATGGAG ACTTGTTCTG TAAAGCATTA
    AAAACCTACA ACATGCTGTG CTTTGGAATC TATAGATTGC GAGATGCACA TTTAGGTGCA
    CCAAGCCAGT GCACAAAAAG ATATGTCATC ACAAACCCGC CTTATGAGTT TGAGATGGTG
    CCCACGGACC TGATCTTCTG TCTGATGCAG TTCGACCACA ACGCGGGCCA GTCCCGAGCG
    AGCCTGTCCC ACTCCTCCCA TTCGTCCCAT TCCTCCAGCA AGAAGAGCTC GTCTGTTCAC
    TCCATCCCAG CCACCAACCG GCAGAACCGC AGCAGCAAAG CACGTGAAGC CCGTGACAAA
    CAGAAAAAAG AGATGGTTTA CAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214274.1
    CDS653..4099
    Translation

    Target ORF information:

    RefSeq Version XM_017358785.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X26, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358785.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATCGAA GATGAGCATC CGTCAACACT GTCACCCAAA
    AAGAAGCAGC GCAACGGAGG CATGCGAAAT TCACCCAACT GCTCGCCCAA AATGATGAGG
    CATGATCCTC TACTCATTCC AGGAAATGAG CAGATTGAGA GCATGGATGC TAATGTCAAA
    CGATACGACT CTACTGGAAT GTTTCACTGG TGTCCATCCA AAGAAATCGA GAAGGTCATT
    CTGACACGCA GCGAGGCCTC AATGACGGTT TTAAGTGGAC ATGTCGTAGT CTGCATATTT
    GGAGATGTCA CGTCAGCTTT AGTGGGGCTT CGAAACTTAG TCATGCCTTT GAGAGCAAGC
    AACTTCCATT ACCATGAGCT AAAGCCCATT GTGTTTGTGG GCTCGTTAGA TTATCTGCGA
    AGAGAGTGGG AAACCCTTCA CAACTTCCCC AAAGTCTTCA TCTTACCTGG TACGCCATTA
    AGTCGAGCTG ATTTAAGGGC CGTCAACATC AACCTCTGCG ACATGTGCGT TATCCTGTCA
    GCCAATCAGA ACAATATCGA CGATGCTTCA CTGCAGGACA AGGAATGCAT CTTGGCATCT
    CTTAACATCA AATCTATGCA GTTTGATGAC AGCATTGGGG TCTTGCAGGC TAATTCCCAA
    GGTTTCACAC CTCCAGGGAT GGATAGGTCA TCTCCTGATA ACAGTCCCGT GCACGGCTTA
    GTGCGGCAGG CATCCGTCAC CACCGGATCA AACATCCCCA TCATAACTGA ACTGGTGAAC
    GACTCCAACG TTCAGTTTTT GGACCAAGAC GACGATGACG ACCCAGACAC TGAGCTCTAC
    CTGACCCAGC CGTTCGCCTG TGGTACCGCA TTCGCCGTCA GCGTGTTGGA CTCCCTGATG
    AGTGCGACAT ACTTCAATGA TAATATCCTC ACCCTGATCC GGACGCTGGT CACGGGAGGC
    GCCACGCCGG AGCTGGAGGC CCTGTTGGCT GAGGAGAACG CTCTGAGAGG TGGCTACAGC
    ACGCCGCAGA CGCTGGCTAA CAGGGACAGG TGTCGGGTCG CCCAGCTGGC CCTCTACGAT
    GGGCCCTTCG CAGACTTGGG GGACGGAGGT TGCTATGGAG ACTTGTTCTG TAAAGCATTA
    AAAACCTACA ACATGCTGTG CTTTGGAATC TATAGATTGC GAGATGCACA TTTAGGTGCA
    CCAAGCCAGT GCACAAAAAG ATATGTCATC ACAAACCCGC CTTATGAGTT TGAGATGGTG
    CCCACGGACC TGATCTTCTG TCTGATGCAG TTCGACCACA ACGCGGGCCA GTCCCGAGCG
    AGCCTGTCCC ACTCCTCCCA TTCGTCCCAT TCCTCCAGCA AGAAGAGCTC GTCTGTTCAC
    TCCATCCCAG CCACCAACCG GCAGAACCGC AGCAGCAAAG CACGTGAAGC CCGTGACAAA
    CAGAAAAAAG AGATGGTTTA CAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa31868
    Clone ID Related Accession (Same CDS sequence) NM_001145600.1
    Accession Version NM_001145600.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3555bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL645926.16, AL954192.3, CT025847.4 and BX469897.6. On Mar 5, 2009 this sequence version replaced XM_001922937.1. ##Evidence-Data-START## CDS exon combination :: GFIL01013841.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTAATA ATATCAATTT TAATAAAAAT CCGGACTCTT CTGTCTCTAT ATCGAAGATG 
    GACGTGATCA TCCCGTTCAC ACCAGACGTA CCCTGTGACA ACAATGGTCA GCGCATGTGG
    TGGGCTTTCC TCGCCTCATC TATGGTGACG TTCTTCGGGG GTCTCTTCAT CATCCTCCTG
    TGGAGGACCC TCAAGTACCT GTGGACTGTC TGCTGCCATT GCAACATCAA AAACAAGGAG
    GCACAGAAGG TGAATAACCC TATTACTATT CAGGCTGATG GCACAACAAA GACTGGGAAT
    GAAAAAGAAG AAGCCCCAGC CAGTGAAGTG GGCTGGATGA CCTCAGTCAA AGACTGGGCC
    GGGGTCATGA TCTCAGCACA GACCCTCACC GGCAGAGTTT TGGTGGTGTT GGTCTTTGCA
    CTCAGCATCG GAGCTCTGGG AATATACTTC ATAGACTCTT CGGATCCTAT AGAATCCTGT
    CAGAATTTCT ACAAAGACTT CACGTTACAA ATTGACATGG CATTCAACGT GTTCTTCTTA
    CTCTACTTTG GTCTGCGCTT CATAGCAGCC AATGATAAGC TGTGGTTCTG GCTGGAGGTC
    AACTCAGTGG TGGACTTCTT CACCGTGCCT CCCGTGTTTG TGTCAGTGTA CCTGAACAGG
    AGTTGGCTTG GTCTGAGGTT TCTAAGAGCC TTGAGATTGA TACAGTTCTC AGAAATTTTA
    CAGTTTTTGA ATATCTTAAA AACAAGTAAT TCCATCAAGC TGGTGAACCT GTGTTCCATC
    TTCATAAGCA CATGGCTCAC TGCTGCAGGA TTCATTCATC TGGTGGAAAA CTCAGGGGAT
    CCATGGGAAA ACTTCCAAAA CTCTCAACCG CTCTCATATT GGGAATGTGT GTACTTATTA
    ATGGTCACCA TGTCCACGGT GGGATATGGG GACGTCTATG CAAGAACCAC TCTTGGACGC
    CTTTTCATGG TCTTCTTCAT TCTCGGTGGT CTGGCCATGT TTGCCAGCTA CGTCCCTGAA
    ATCATAGAGT TAATAGGAAA CCGCAAGAAA TATGGTGGTT CCTATAGTGC GGTAAATGGC
    AGAAAGCATA TTGTGGTCTG TGGTCATATC ACGCTCGAAA GTGTTTCCAA CTTCCTTAAA
    GACTTCCTAC ACAAGGACAG GGATGATGTC AATGTAGAAA TTGTTTTTCT TCATAATATT
    TCCCCTAATC TTGAGCTGGA AGCCTTGTTC AAACGTCACT TCACTCAGGT GGAATTCTAT
    CAAGGATCGG TCCTCAACCC ACATGATCTA GCAAGAGTGA AGATTGAGTC GGCGGACGCT
    TGCTTGATTC TTGCAAACAA ATACTGTGCA GACCCCGATG CTGAGGATGC CTCAAATATC
    ATGAGAGTGA TATCTATCAA GAACTACCAT CCAAAGATCA GAATAATAAC ACAAATGTTG
    CAGTACCACA ACAAGGCCCA TCTGCTAAAC ATTCCCAGCT GGAACTGGAA GGAGGGAGAT
    GATGCGATTT GCCTGGCCGA GTTGAAGGCA GGCTTCATAG CCCAGAGCTG CCTGGCACAG
    GGCCTGTCCA CCATGCTCGC CAACCTCTTC TCCATGAGGT CATACATCAA GATTGAAGAA
    GACACATGGC AGAAGTATTA TCTGGAAGGA GTAGCCAATG AAATGTACAC TGAGTACCTG
    TCTAGTGCCT TTGTCGGCCT TTCATTTCCT ACAGTTTGTG AGCTGTGCTA CGTGAAGCTC
    AAGTTGCTGT TGATCGCTAT TGAGTACAAG TCAGAGCAAA GGGAAAGCAG TATTCTGATC
    AACCCGGGAA ATCACGTTAA AATGCAGGAG GGGACACTGG GATTCTTTAT TGCCAGTGAT
    GCCAAAGAAG TGAAAAGAGC ATTTTTCTAC TGCAAAGCAT GTCATGATGA CATCACAGAT
    CCCAAACGGA TTAAAAAGTG TGGCTGCAAG AGAATCGAAG ATGAGCATCC GTCAACACTG
    TCACCCAAAA AGAAGCAGCG CAACGGAGGC ATGCGAAATT CACCCAACTG CTCGCCCAAA
    ATGATGAGGC ATGATCCTCT ACTCATTCCA GGAAATGAGC AGATTGAGAG CATGGATGCT
    AATGTCAAAC GATACGACTC TACTGGAATG TTTCACTGGT GTCCATCCAA AGAAATCGAG
    AAGGTCATTC TGACACGCAG CGAGGCCTCA ATGACGGTTT TAAGTGGACA TGTCGTAGTC
    TGCATATTTG GAGATGTCAC GTCAGCTTTA GTGGGGCTTC GAAACTTAGT CATGCCTTTG
    AGAGCAAGCA ACTTCCATTA CCATGAGCTA AAGCCCATTG TGTTTGTGGG CTCGTTAGAT
    TATCTGCGAA GAGAGTGGGA AACCCTTCAC AACTTCCCCA AAGTCTTCAT CTTACCTGGT
    ACGCCATTAA GTCGAGCTGA TTTAAGGGCC GTCAACATCA ACCTCTGCGA CATGTGCGTT
    ATCCTGTCAG CCAATCAGAA CAATATCGAC GATGCTTCAC TGCAGGACAA GGAATGCATC
    TTGGCATCTC TTAACATCAA ATCTATGCAG TTTGATGACA GCATTGGGGT CTTGCAGGCT
    AATTCCCAAG GTTTCACACC TCCAGGGATG GATAGGTCAT CTCCTGATAA CAGTCCCGTG
    CACGGCTTAG TGCGGCAGGC ATCCGTCACC ACCGGATCAA ACATCCCCAT CATAACTGAA
    CTGGTGAACG ACTCCAACGT TCAGTTTTTG GACCAAGACG ACGATGACGA CCCAGACACT
    GAGCTCTACC TGACCCAGCC GTTCGCCTGT GGTACCGCAT TCGCCGTCAG CGTGTTGGAC
    TCCCTGATGA GTGCGACATA CTTCAATGAT AATATCCTCA CCCTGATCCG GACGCTGGTC
    ACGGGAGGCG CCACGCCGGA GCTGGAGGCC CTGTTGGCTG AGGAGAACGC TCTGAGAGGT
    GGCTACAGCA CGCCGCAGAC GCTGGCTAAC AGGGACAGGT GTCGGGTCGC CCAGCTGGCC
    CTCTACGATG GGCCCTTCGC AGACTTGGGG GACGGAGGTT GCTATGGAGA CTTGTTCTGT
    AAAGCATTAA AAACCTACAA CATGCTGTGC TTTGGAATCT ATAGATTGCG AGATGCACAT
    TTAGGTGCAC CAAGCCAGTG CACAAAAAGA TATGTCATCA CAAACCCGCC TTATGAGTTT
    GAGATGGTGC CCACGGACCT GATCTTCTGT CTGATGCAGT TCGACCACAA CGCGGGCCAG
    TCCCGAGCGA GCCTGTCCCA CTCCTCCCAT TCGTCCCATT CCTCCAGCAA GAAGAGCTCG
    TCTGTTCACT CCATCCCAGC CACCAACCGG CAGAACCGCA GCAGCAAAGC ACGTGAAGCC
    CGTGACAAAC AGAATGCAAC AAGGATGAAT AGAATGGGCC CAGAAAAGAG ATGGTTTACA
    GATGAAGCAG AAAACGCATA CCCAAGGAAC ATTCAAATCA AGCCTATGAG CACTCACATG
    GCCAACCAAG TCAATCAGTA CAAATCCACT AGCAGTCTGA TTCCACCTAT CCGAGAAGTC
    GAAGACGAAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001139072.1
    CDS1..3555
    Translation

    Target ORF information:

    RefSeq Version NM_001145600.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001145600.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGAGTAATA ATATCAATTT TAATAAAAAT CCGGACTCTT CTGTCTCTAT ATCGAAGATG 
    GACGTGATCA TCCCGTTCAC ACCAGACGTA CCCTGTGACA ACAATGGTCA GCGCATGTGG
    TGGGCTTTCC TCGCCTCATC TATGGTGACG TTCTTCGGGG GTCTCTTCAT CATCCTCCTG
    TGGAGGACCC TCAAGTACCT GTGGACTGTC TGCTGCCATT GCAACATCAA AAACAAGGAG
    GCACAGAAGG TGAATAACCC TATTACTATT CAGGCTGATG GCACAACAAA GACTGGGAAT
    GAAAAAGAAG AAGCCCCAGC CAGTGAAGTG GGCTGGATGA CCTCAGTCAA AGACTGGGCC
    GGGGTCATGA TCTCAGCACA GACCCTCACC GGCAGAGTTT TGGTGGTGTT GGTCTTTGCA
    CTCAGCATCG GAGCTCTGGG AATATACTTC ATAGACTCTT CGGATCCTAT AGAATCCTGT
    CAGAATTTCT ACAAAGACTT CACGTTACAA ATTGACATGG CATTCAACGT GTTCTTCTTA
    CTCTACTTTG GTCTGCGCTT CATAGCAGCC AATGATAAGC TGTGGTTCTG GCTGGAGGTC
    AACTCAGTGG TGGACTTCTT CACCGTGCCT CCCGTGTTTG TGTCAGTGTA CCTGAACAGG
    AGTTGGCTTG GTCTGAGGTT TCTAAGAGCC TTGAGATTGA TACAGTTCTC AGAAATTTTA
    CAGTTTTTGA ATATCTTAAA AACAAGTAAT TCCATCAAGC TGGTGAACCT GTGTTCCATC
    TTCATAAGCA CATGGCTCAC TGCTGCAGGA TTCATTCATC TGGTGGAAAA CTCAGGGGAT
    CCATGGGAAA ACTTCCAAAA CTCTCAACCG CTCTCATATT GGGAATGTGT GTACTTATTA
    ATGGTCACCA TGTCCACGGT GGGATATGGG GACGTCTATG CAAGAACCAC TCTTGGACGC
    CTTTTCATGG TCTTCTTCAT TCTCGGTGGT CTGGCCATGT TTGCCAGCTA CGTCCCTGAA
    ATCATAGAGT TAATAGGAAA CCGCAAGAAA TATGGTGGTT CCTATAGTGC GGTAAATGGC
    AGAAAGCATA TTGTGGTCTG TGGTCATATC ACGCTCGAAA GTGTTTCCAA CTTCCTTAAA
    GACTTCCTAC ACAAGGACAG GGATGATGTC AATGTAGAAA TTGTTTTTCT TCATAATATT
    TCCCCTAATC TTGAGCTGGA AGCCTTGTTC AAACGTCACT TCACTCAGGT GGAATTCTAT
    CAAGGATCGG TCCTCAACCC ACATGATCTA GCAAGAGTGA AGATTGAGTC GGCGGACGCT
    TGCTTGATTC TTGCAAACAA ATACTGTGCA GACCCCGATG CTGAGGATGC CTCAAATATC
    ATGAGAGTGA TATCTATCAA GAACTACCAT CCAAAGATCA GAATAATAAC ACAAATGTTG
    CAGTACCACA ACAAGGCCCA TCTGCTAAAC ATTCCCAGCT GGAACTGGAA GGAGGGAGAT
    GATGCGATTT GCCTGGCCGA GTTGAAGGCA GGCTTCATAG CCCAGAGCTG CCTGGCACAG
    GGCCTGTCCA CCATGCTCGC CAACCTCTTC TCCATGAGGT CATACATCAA GATTGAAGAA
    GACACATGGC AGAAGTATTA TCTGGAAGGA GTAGCCAATG AAATGTACAC TGAGTACCTG
    TCTAGTGCCT TTGTCGGCCT TTCATTTCCT ACAGTTTGTG AGCTGTGCTA CGTGAAGCTC
    AAGTTGCTGT TGATCGCTAT TGAGTACAAG TCAGAGCAAA GGGAAAGCAG TATTCTGATC
    AACCCGGGAA ATCACGTTAA AATGCAGGAG GGGACACTGG GATTCTTTAT TGCCAGTGAT
    GCCAAAGAAG TGAAAAGAGC ATTTTTCTAC TGCAAAGCAT GTCATGATGA CATCACAGAT
    CCCAAACGGA TTAAAAAGTG TGGCTGCAAG AGAATCGAAG ATGAGCATCC GTCAACACTG
    TCACCCAAAA AGAAGCAGCG CAACGGAGGC ATGCGAAATT CACCCAACTG CTCGCCCAAA
    ATGATGAGGC ATGATCCTCT ACTCATTCCA GGAAATGAGC AGATTGAGAG CATGGATGCT
    AATGTCAAAC GATACGACTC TACTGGAATG TTTCACTGGT GTCCATCCAA AGAAATCGAG
    AAGGTCATTC TGACACGCAG CGAGGCCTCA ATGACGGTTT TAAGTGGACA TGTCGTAGTC
    TGCATATTTG GAGATGTCAC GTCAGCTTTA GTGGGGCTTC GAAACTTAGT CATGCCTTTG
    AGAGCAAGCA ACTTCCATTA CCATGAGCTA AAGCCCATTG TGTTTGTGGG CTCGTTAGAT
    TATCTGCGAA GAGAGTGGGA AACCCTTCAC AACTTCCCCA AAGTCTTCAT CTTACCTGGT
    ACGCCATTAA GTCGAGCTGA TTTAAGGGCC GTCAACATCA ACCTCTGCGA CATGTGCGTT
    ATCCTGTCAG CCAATCAGAA CAATATCGAC GATGCTTCAC TGCAGGACAA GGAATGCATC
    TTGGCATCTC TTAACATCAA ATCTATGCAG TTTGATGACA GCATTGGGGT CTTGCAGGCT
    AATTCCCAAG GTTTCACACC TCCAGGGATG GATAGGTCAT CTCCTGATAA CAGTCCCGTG
    CACGGCTTAG TGCGGCAGGC ATCCGTCACC ACCGGATCAA ACATCCCCAT CATAACTGAA
    CTGGTGAACG ACTCCAACGT TCAGTTTTTG GACCAAGACG ACGATGACGA CCCAGACACT
    GAGCTCTACC TGACCCAGCC GTTCGCCTGT GGTACCGCAT TCGCCGTCAG CGTGTTGGAC
    TCCCTGATGA GTGCGACATA CTTCAATGAT AATATCCTCA CCCTGATCCG GACGCTGGTC
    ACGGGAGGCG CCACGCCGGA GCTGGAGGCC CTGTTGGCTG AGGAGAACGC TCTGAGAGGT
    GGCTACAGCA CGCCGCAGAC GCTGGCTAAC AGGGACAGGT GTCGGGTCGC CCAGCTGGCC
    CTCTACGATG GGCCCTTCGC AGACTTGGGG GACGGAGGTT GCTATGGAGA CTTGTTCTGT
    AAAGCATTAA AAACCTACAA CATGCTGTGC TTTGGAATCT ATAGATTGCG AGATGCACAT
    TTAGGTGCAC CAAGCCAGTG CACAAAAAGA TATGTCATCA CAAACCCGCC TTATGAGTTT
    GAGATGGTGC CCACGGACCT GATCTTCTGT CTGATGCAGT TCGACCACAA CGCGGGCCAG
    TCCCGAGCGA GCCTGTCCCA CTCCTCCCAT TCGTCCCATT CCTCCAGCAA GAAGAGCTCG
    TCTGTTCACT CCATCCCAGC CACCAACCGG CAGAACCGCA GCAGCAAAGC ACGTGAAGCC
    CGTGACAAAC AGAATGCAAC AAGGATGAAT AGAATGGGCC CAGAAAAGAG ATGGTTTACA
    GATGAAGCAG AAAACGCATA CCCAAGGAAC ATTCAAATCA AGCCTATGAG CACTCACATG
    GCCAACCAAG TCAATCAGTA CAAATCCACT AGCAGTCTGA TTCCACCTAT CCGAGAAGTC
    GAAGACGAAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18608
    Clone ID Related Accession (Same CDS sequence) XM_005156655.4 , XM_005156655.3
    Accession Version XM_005156655.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156655.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT TCGAAGATGA GCATCCGTCA
    ACACTGTCAC CCAAAAAGAA GCAGCGCAAC GGAGGCATGC GAAATTCACC CAACTGCTCG
    CCCAAAATGA TGAGGCATGA TCCTCTACTC ATTCCAGGAA ATGAGCAGAT TGAGAGCATG
    GATGCTAATG TCAAACGATA CGACTCTACT GGAATGTTTC ACTGGTGTCC ATCCAAAGAA
    ATCGAGAAGG TCATTCTGAC ACGCAGCGAG GCCTCAATGA CGGTTTTAAG TGGACATGTC
    GTAGTCTGCA TATTTGGAGA TGTCACGTCA GCTTTAGTGG GGCTTCGAAA CTTAGTCATG
    CCTTTGAGAG CAAGCAACTT CCATTACCAT GAGCTAAAGC CCATTGTGTT TGTGGGCTCG
    TTAGATTATC TGCGAAGAGA GTGGGAAACC CTTCACAACT TCCCCAAAGT CTTCATCTTA
    CCTGGTACGC CATTAAGTCG AGCTGATTTA AGGGCCGTCA ACATCAACCT CTGCGACATG
    TGCGTTATCC TGTCAGCCAA TCAGAACAAT ATCGACGATG CTTCACTGCA GGACAAGGAA
    TGCATCTTGG CATCTCTTAA CATCAAATCT ATGCAGTTTG ATGACAGCAT TGGGGTCTTG
    CAGGCTAATT CCCAAGGTTT CACACCTCCA GGGATGGATA GGTCATCTCC TGATAACAGT
    CCCGTGCACG GCTTAGTGCG GCAGGCATCC GTCACCACCG GATCAAACAT CCCCATCATA
    ACTGAACTGG CTAAGCCTGG CAAACTACTG CCTTTGGTTT CATTCAGTCA GGAAAAAAAC
    AGTGGAACCA ACATACAAAT GATAACAGAA CTGGTGAACG ACTCCAACGT TCAGTTTTTG
    GACCAAGACG ACGATGACGA CCCAGACACT GAGCTCTACC TGACCCAGCC GTTCGCCTGT
    GGTACCGCAT TCGCCGTCAG CGTGTTGGAC TCCCTGATGA GTGCGACATA CTTCAATGAT
    AATATCCTCA CCCTGATCCG GACGCTGGTC ACGGGAGGCG CCACGCCGGA GCTGGAGGCC
    CTGTTGGCTG AGGAGAACGC TCTGAGAGGT GGCTACAGCA CGCCGCAGAC GCTGGCTAAC
    AGGGACAGGT GTCGGGTCGC CCAGCTGGCC CTCTACGATG GGCCCTTCGC AGACTTGGGG
    GACGGAGGTT GCTATGGAGA CTTGTTCTGT AAAGCATTAA AAACCTACAA CATGCTGTGC
    TTTGGAATCT ATAGATTGCG AGATGCACAT TTAGGTGCAC CAAGCCAGTG CACAAAAAGA
    TATGTCATCA CAAACCCGCC TTATGAGTTT GAGATGGTGC CCACGGACCT GATCTTCTGT
    CTGATGCAGT TCGACCACAA CGCGGGCCAG TCCCGAGCGA GCCTGTCCCA CTCCTCCCAT
    TCGTCCCATT CCTCCAGCAA GAAGAGCTCG TCTGTTCACT CCATCCCAGC CACCAACCGG
    CAGAACCGCA GCAGCAAAGC ACGTGAAGCC CGTGACAAAC AGAATGCAAC AAGGATGAAT
    AGAATGGGCC CAGAAAAGAG ATGGTTTACA GATGAAGCAG AAAACGCATA CCCAAGGAAC
    ATTCAAATCA AGCCTATGAG CACTCACATG GCCAACCAAG TCAATCAGTA CAAATCCACT
    AGCAGTCTGA TTCCACCTAT CCGAGAAGTC GAAGACGAAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156712.1
    CDS677..4561
    Translation

    Target ORF information:

    RefSeq Version XM_005156655.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156655.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT TCGAAGATGA GCATCCGTCA
    ACACTGTCAC CCAAAAAGAA GCAGCGCAAC GGAGGCATGC GAAATTCACC CAACTGCTCG
    CCCAAAATGA TGAGGCATGA TCCTCTACTC ATTCCAGGAA ATGAGCAGAT TGAGAGCATG
    GATGCTAATG TCAAACGATA CGACTCTACT GGAATGTTTC ACTGGTGTCC ATCCAAAGAA
    ATCGAGAAGG TCATTCTGAC ACGCAGCGAG GCCTCAATGA CGGTTTTAAG TGGACATGTC
    GTAGTCTGCA TATTTGGAGA TGTCACGTCA GCTTTAGTGG GGCTTCGAAA CTTAGTCATG
    CCTTTGAGAG CAAGCAACTT CCATTACCAT GAGCTAAAGC CCATTGTGTT TGTGGGCTCG
    TTAGATTATC TGCGAAGAGA GTGGGAAACC CTTCACAACT TCCCCAAAGT CTTCATCTTA
    CCTGGTACGC CATTAAGTCG AGCTGATTTA AGGGCCGTCA ACATCAACCT CTGCGACATG
    TGCGTTATCC TGTCAGCCAA TCAGAACAAT ATCGACGATG CTTCACTGCA GGACAAGGAA
    TGCATCTTGG CATCTCTTAA CATCAAATCT ATGCAGTTTG ATGACAGCAT TGGGGTCTTG
    CAGGCTAATT CCCAAGGTTT CACACCTCCA GGGATGGATA GGTCATCTCC TGATAACAGT
    CCCGTGCACG GCTTAGTGCG GCAGGCATCC GTCACCACCG GATCAAACAT CCCCATCATA
    ACTGAACTGG CTAAGCCTGG CAAACTACTG CCTTTGGTTT CATTCAGTCA GGAAAAAAAC
    AGTGGAACCA ACATACAAAT GATAACAGAA CTGGTGAACG ACTCCAACGT TCAGTTTTTG
    GACCAAGACG ACGATGACGA CCCAGACACT GAGCTCTACC TGACCCAGCC GTTCGCCTGT
    GGTACCGCAT TCGCCGTCAG CGTGTTGGAC TCCCTGATGA GTGCGACATA CTTCAATGAT
    AATATCCTCA CCCTGATCCG GACGCTGGTC ACGGGAGGCG CCACGCCGGA GCTGGAGGCC
    CTGTTGGCTG AGGAGAACGC TCTGAGAGGT GGCTACAGCA CGCCGCAGAC GCTGGCTAAC
    AGGGACAGGT GTCGGGTCGC CCAGCTGGCC CTCTACGATG GGCCCTTCGC AGACTTGGGG
    GACGGAGGTT GCTATGGAGA CTTGTTCTGT AAAGCATTAA AAACCTACAA CATGCTGTGC
    TTTGGAATCT ATAGATTGCG AGATGCACAT TTAGGTGCAC CAAGCCAGTG CACAAAAAGA
    TATGTCATCA CAAACCCGCC TTATGAGTTT GAGATGGTGC CCACGGACCT GATCTTCTGT
    CTGATGCAGT TCGACCACAA CGCGGGCCAG TCCCGAGCGA GCCTGTCCCA CTCCTCCCAT
    TCGTCCCATT CCTCCAGCAA GAAGAGCTCG TCTGTTCACT CCATCCCAGC CACCAACCGG
    CAGAACCGCA GCAGCAAAGC ACGTGAAGCC CGTGACAAAC AGAATGCAAC AAGGATGAAT
    AGAATGGGCC CAGAAAAGAG ATGGTTTACA GATGAAGCAG AAAACGCATA CCCAAGGAAC
    ATTCAAATCA AGCCTATGAG CACTCACATG GCCAACCAAG TCAATCAGTA CAAATCCACT
    AGCAGTCTGA TTCCACCTAT CCGAGAAGTC GAAGACGAAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18608
    Clone ID Related Accession (Same CDS sequence) XM_005156655.4 , XM_005156655.3
    Accession Version XM_005156655.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156655.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT TCGAAGATGA GCATCCGTCA
    ACACTGTCAC CCAAAAAGAA GCAGCGCAAC GGAGGCATGC GAAATTCACC CAACTGCTCG
    CCCAAAATGA TGAGGCATGA TCCTCTACTC ATTCCAGGAA ATGAGCAGAT TGAGAGCATG
    GATGCTAATG TCAAACGATA CGACTCTACT GGAATGTTTC ACTGGTGTCC ATCCAAAGAA
    ATCGAGAAGG TCATTCTGAC ACGCAGCGAG GCCTCAATGA CGGTTTTAAG TGGACATGTC
    GTAGTCTGCA TATTTGGAGA TGTCACGTCA GCTTTAGTGG GGCTTCGAAA CTTAGTCATG
    CCTTTGAGAG CAAGCAACTT CCATTACCAT GAGCTAAAGC CCATTGTGTT TGTGGGCTCG
    TTAGATTATC TGCGAAGAGA GTGGGAAACC CTTCACAACT TCCCCAAAGT CTTCATCTTA
    CCTGGTACGC CATTAAGTCG AGCTGATTTA AGGGCCGTCA ACATCAACCT CTGCGACATG
    TGCGTTATCC TGTCAGCCAA TCAGAACAAT ATCGACGATG CTTCACTGCA GGACAAGGAA
    TGCATCTTGG CATCTCTTAA CATCAAATCT ATGCAGTTTG ATGACAGCAT TGGGGTCTTG
    CAGGCTAATT CCCAAGGTTT CACACCTCCA GGGATGGATA GGTCATCTCC TGATAACAGT
    CCCGTGCACG GCTTAGTGCG GCAGGCATCC GTCACCACCG GATCAAACAT CCCCATCATA
    ACTGAACTGG CTAAGCCTGG CAAACTACTG CCTTTGGTTT CATTCAGTCA GGAAAAAAAC
    AGTGGAACCA ACATACAAAT GATAACAGAA CTGGTGAACG ACTCCAACGT TCAGTTTTTG
    GACCAAGACG ACGATGACGA CCCAGACACT GAGCTCTACC TGACCCAGCC GTTCGCCTGT
    GGTACCGCAT TCGCCGTCAG CGTGTTGGAC TCCCTGATGA GTGCGACATA CTTCAATGAT
    AATATCCTCA CCCTGATCCG GACGCTGGTC ACGGGAGGCG CCACGCCGGA GCTGGAGGCC
    CTGTTGGCTG AGGAGAACGC TCTGAGAGGT GGCTACAGCA CGCCGCAGAC GCTGGCTAAC
    AGGGACAGGT GTCGGGTCGC CCAGCTGGCC CTCTACGATG GGCCCTTCGC AGACTTGGGG
    GACGGAGGTT GCTATGGAGA CTTGTTCTGT AAAGCATTAA AAACCTACAA CATGCTGTGC
    TTTGGAATCT ATAGATTGCG AGATGCACAT TTAGGTGCAC CAAGCCAGTG CACAAAAAGA
    TATGTCATCA CAAACCCGCC TTATGAGTTT GAGATGGTGC CCACGGACCT GATCTTCTGT
    CTGATGCAGT TCGACCACAA CGCGGGCCAG TCCCGAGCGA GCCTGTCCCA CTCCTCCCAT
    TCGTCCCATT CCTCCAGCAA GAAGAGCTCG TCTGTTCACT CCATCCCAGC CACCAACCGG
    CAGAACCGCA GCAGCAAAGC ACGTGAAGCC CGTGACAAAC AGAATGCAAC AAGGATGAAT
    AGAATGGGCC CAGAAAAGAG ATGGTTTACA GATGAAGCAG AAAACGCATA CCCAAGGAAC
    ATTCAAATCA AGCCTATGAG CACTCACATG GCCAACCAAG TCAATCAGTA CAAATCCACT
    AGCAGTCTGA TTCCACCTAT CCGAGAAGTC GAAGACGAAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156712.1
    CDS658..4542
    Translation

    Target ORF information:

    RefSeq Version XM_005156655.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156655.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT TCGAAGATGA GCATCCGTCA
    ACACTGTCAC CCAAAAAGAA GCAGCGCAAC GGAGGCATGC GAAATTCACC CAACTGCTCG
    CCCAAAATGA TGAGGCATGA TCCTCTACTC ATTCCAGGAA ATGAGCAGAT TGAGAGCATG
    GATGCTAATG TCAAACGATA CGACTCTACT GGAATGTTTC ACTGGTGTCC ATCCAAAGAA
    ATCGAGAAGG TCATTCTGAC ACGCAGCGAG GCCTCAATGA CGGTTTTAAG TGGACATGTC
    GTAGTCTGCA TATTTGGAGA TGTCACGTCA GCTTTAGTGG GGCTTCGAAA CTTAGTCATG
    CCTTTGAGAG CAAGCAACTT CCATTACCAT GAGCTAAAGC CCATTGTGTT TGTGGGCTCG
    TTAGATTATC TGCGAAGAGA GTGGGAAACC CTTCACAACT TCCCCAAAGT CTTCATCTTA
    CCTGGTACGC CATTAAGTCG AGCTGATTTA AGGGCCGTCA ACATCAACCT CTGCGACATG
    TGCGTTATCC TGTCAGCCAA TCAGAACAAT ATCGACGATG CTTCACTGCA GGACAAGGAA
    TGCATCTTGG CATCTCTTAA CATCAAATCT ATGCAGTTTG ATGACAGCAT TGGGGTCTTG
    CAGGCTAATT CCCAAGGTTT CACACCTCCA GGGATGGATA GGTCATCTCC TGATAACAGT
    CCCGTGCACG GCTTAGTGCG GCAGGCATCC GTCACCACCG GATCAAACAT CCCCATCATA
    ACTGAACTGG CTAAGCCTGG CAAACTACTG CCTTTGGTTT CATTCAGTCA GGAAAAAAAC
    AGTGGAACCA ACATACAAAT GATAACAGAA CTGGTGAACG ACTCCAACGT TCAGTTTTTG
    GACCAAGACG ACGATGACGA CCCAGACACT GAGCTCTACC TGACCCAGCC GTTCGCCTGT
    GGTACCGCAT TCGCCGTCAG CGTGTTGGAC TCCCTGATGA GTGCGACATA CTTCAATGAT
    AATATCCTCA CCCTGATCCG GACGCTGGTC ACGGGAGGCG CCACGCCGGA GCTGGAGGCC
    CTGTTGGCTG AGGAGAACGC TCTGAGAGGT GGCTACAGCA CGCCGCAGAC GCTGGCTAAC
    AGGGACAGGT GTCGGGTCGC CCAGCTGGCC CTCTACGATG GGCCCTTCGC AGACTTGGGG
    GACGGAGGTT GCTATGGAGA CTTGTTCTGT AAAGCATTAA AAACCTACAA CATGCTGTGC
    TTTGGAATCT ATAGATTGCG AGATGCACAT TTAGGTGCAC CAAGCCAGTG CACAAAAAGA
    TATGTCATCA CAAACCCGCC TTATGAGTTT GAGATGGTGC CCACGGACCT GATCTTCTGT
    CTGATGCAGT TCGACCACAA CGCGGGCCAG TCCCGAGCGA GCCTGTCCCA CTCCTCCCAT
    TCGTCCCATT CCTCCAGCAA GAAGAGCTCG TCTGTTCACT CCATCCCAGC CACCAACCGG
    CAGAACCGCA GCAGCAAAGC ACGTGAAGCC CGTGACAAAC AGAATGCAAC AAGGATGAAT
    AGAATGGGCC CAGAAAAGAG ATGGTTTACA GATGAAGCAG AAAACGCATA CCCAAGGAAC
    ATTCAAATCA AGCCTATGAG CACTCACATG GCCAACCAAG TCAATCAGTA CAAATCCACT
    AGCAGTCTGA TTCCACCTAT CCGAGAAGTC GAAGACGAAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18611
    Clone ID Related Accession (Same CDS sequence) XM_005156658.3 , XM_005156658.4
    Accession Version XM_005156658.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3801bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156658.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT TCGAAGATGA GCATCCGTCA
    ACACTGTCAC CCAAAAAGAA GCAGCGCAAC GGAGGCATGC GAAATTCACC CAACTGCTCG
    CCCAAAATGA TGAGGCATGA TCCTCTACTC ATTCCAGGAA ATGAGCAGAT TGAGAGCATG
    GATGCTAATG TCAAACGATA CGACTCTACT GGAATGTTTC ACTGGTGTCC ATCCAAAGAA
    ATCGAGAAGG TCATTCTGAC ACGCAGCGAG GCCTCAATGA CGGTTTTAAG TGGACATGTC
    GTAGTCTGCA TATTTGGAGA TGTCACGTCA GCTTTAGTGG GGCTTCGAAA CTTAGTCATG
    CCTTTGAGAG CAAGCAACTT CCATTACCAT GAGCTAAAGC CCATTGTGTT TGTGGGCTCG
    TTAGATTATC TGCGAAGAGA GTGGGAAACC CTTCACAACT TCCCCAAAGT CTTCATCTTA
    CCTGGTACGC CATTAAGTCG AGCTGATTTA AGGGCCGTCA ACATCAACCT CTGCGACATG
    TGCGTTATCC TGTCAGCCAA TCAGAACAAT ATCGACGATG CTTCACTGCA GGACAAGGAA
    TGCATCTTGG CATCTCTTAA CATCAAATCT ATGCAGTTTG ATGACAGCAT TGGGGTCTTG
    CAGGCTAATT CCCAAGGTTT CACACCTCCA GGGATGGATA GGTCATCTCC TGATAACAGT
    CCCGTGCACG GCTTAGTGCG GCAGGCATCC GTCACCACCG GATCAAACAT CCCCATCATA
    ACTGAACTGG TGAACGACTC CAACGTTCAG TTTTTGGACC AAGACGACGA TGACGACCCA
    GACACTGAGC TCTACCTGAC CCAGCCGTTC GCCTGTGGTA CCGCATTCGC CGTCAGCGTG
    TTGGACTCCC TGATGAGTGC GACATACTTC AATGATAATA TCCTCACCCT GATCCGGACG
    CTGGTCACGG GAGGCGCCAC GCCGGAGCTG GAGGCCCTGT TGGCTGAGGA GAACGCTCTG
    AGAGGTGGCT ACAGCACGCC GCAGACGCTG GCTAACAGGG ACAGGTGTCG GGTCGCCCAG
    CTGGCCCTCT ACGATGGGCC CTTCGCAGAC TTGGGGGACG GAGGTTGCTA TGGAGACTTG
    TTCTGTAAAG CATTAAAAAC CTACAACATG CTGTGCTTTG GAATCTATAG ATTGCGAGAT
    GCACATTTAG GTGCACCAAG CCAGTGCACA AAAAGATATG TCATCACAAA CCCGCCTTAT
    GAGTTTGAGA TGGTGCCCAC GGACCTGATC TTCTGTCTGA TGCAGTTCGA CCACAACGCG
    GGCCAGTCCC GAGCGAGCCT GTCCCACTCC TCCCATTCGT CCCATTCCTC CAGCAAGAAG
    AGCTCGTCTG TTCACTCCAT CCCAGCCACC AACCGGCAGA ACCGCAGCAG CAAAGCACGT
    GAAGCCCGTG ACAAACAGAA TGCAACAAGG ATGAATAGAA TGGGCCCAGA AAAGAGATGG
    TTTACAGATG AAGCAGAAAA CGCATACCCA AGGAACATTC AAATCAAGCC TATGAGCACT
    CACATGGCCA ACCAAGTCAA TCAGTACAAA TCCACTAGCA GTCTGATTCC ACCTATCCGA
    GAAGTCGAAG ACGAATGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156715.1
    CDS654..4454
    Translation

    Target ORF information:

    RefSeq Version XM_005156658.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156658.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT TCGAAGATGA GCATCCGTCA
    ACACTGTCAC CCAAAAAGAA GCAGCGCAAC GGAGGCATGC GAAATTCACC CAACTGCTCG
    CCCAAAATGA TGAGGCATGA TCCTCTACTC ATTCCAGGAA ATGAGCAGAT TGAGAGCATG
    GATGCTAATG TCAAACGATA CGACTCTACT GGAATGTTTC ACTGGTGTCC ATCCAAAGAA
    ATCGAGAAGG TCATTCTGAC ACGCAGCGAG GCCTCAATGA CGGTTTTAAG TGGACATGTC
    GTAGTCTGCA TATTTGGAGA TGTCACGTCA GCTTTAGTGG GGCTTCGAAA CTTAGTCATG
    CCTTTGAGAG CAAGCAACTT CCATTACCAT GAGCTAAAGC CCATTGTGTT TGTGGGCTCG
    TTAGATTATC TGCGAAGAGA GTGGGAAACC CTTCACAACT TCCCCAAAGT CTTCATCTTA
    CCTGGTACGC CATTAAGTCG AGCTGATTTA AGGGCCGTCA ACATCAACCT CTGCGACATG
    TGCGTTATCC TGTCAGCCAA TCAGAACAAT ATCGACGATG CTTCACTGCA GGACAAGGAA
    TGCATCTTGG CATCTCTTAA CATCAAATCT ATGCAGTTTG ATGACAGCAT TGGGGTCTTG
    CAGGCTAATT CCCAAGGTTT CACACCTCCA GGGATGGATA GGTCATCTCC TGATAACAGT
    CCCGTGCACG GCTTAGTGCG GCAGGCATCC GTCACCACCG GATCAAACAT CCCCATCATA
    ACTGAACTGG TGAACGACTC CAACGTTCAG TTTTTGGACC AAGACGACGA TGACGACCCA
    GACACTGAGC TCTACCTGAC CCAGCCGTTC GCCTGTGGTA CCGCATTCGC CGTCAGCGTG
    TTGGACTCCC TGATGAGTGC GACATACTTC AATGATAATA TCCTCACCCT GATCCGGACG
    CTGGTCACGG GAGGCGCCAC GCCGGAGCTG GAGGCCCTGT TGGCTGAGGA GAACGCTCTG
    AGAGGTGGCT ACAGCACGCC GCAGACGCTG GCTAACAGGG ACAGGTGTCG GGTCGCCCAG
    CTGGCCCTCT ACGATGGGCC CTTCGCAGAC TTGGGGGACG GAGGTTGCTA TGGAGACTTG
    TTCTGTAAAG CATTAAAAAC CTACAACATG CTGTGCTTTG GAATCTATAG ATTGCGAGAT
    GCACATTTAG GTGCACCAAG CCAGTGCACA AAAAGATATG TCATCACAAA CCCGCCTTAT
    GAGTTTGAGA TGGTGCCCAC GGACCTGATC TTCTGTCTGA TGCAGTTCGA CCACAACGCG
    GGCCAGTCCC GAGCGAGCCT GTCCCACTCC TCCCATTCGT CCCATTCCTC CAGCAAGAAG
    AGCTCGTCTG TTCACTCCAT CCCAGCCACC AACCGGCAGA ACCGCAGCAG CAAAGCACGT
    GAAGCCCGTG ACAAACAGAA TGCAACAAGG ATGAATAGAA TGGGCCCAGA AAAGAGATGG
    TTTACAGATG AAGCAGAAAA CGCATACCCA AGGAACATTC AAATCAAGCC TATGAGCACT
    CACATGGCCA ACCAAGTCAA TCAGTACAAA TCCACTAGCA GTCTGATTCC ACCTATCCGA
    GAAGTCGAAG ACGAATGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18611
    Clone ID Related Accession (Same CDS sequence) XM_005156658.3 , XM_005156658.4
    Accession Version XM_005156658.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3801bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156658.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT TCGAAGATGA GCATCCGTCA
    ACACTGTCAC CCAAAAAGAA GCAGCGCAAC GGAGGCATGC GAAATTCACC CAACTGCTCG
    CCCAAAATGA TGAGGCATGA TCCTCTACTC ATTCCAGGAA ATGAGCAGAT TGAGAGCATG
    GATGCTAATG TCAAACGATA CGACTCTACT GGAATGTTTC ACTGGTGTCC ATCCAAAGAA
    ATCGAGAAGG TCATTCTGAC ACGCAGCGAG GCCTCAATGA CGGTTTTAAG TGGACATGTC
    GTAGTCTGCA TATTTGGAGA TGTCACGTCA GCTTTAGTGG GGCTTCGAAA CTTAGTCATG
    CCTTTGAGAG CAAGCAACTT CCATTACCAT GAGCTAAAGC CCATTGTGTT TGTGGGCTCG
    TTAGATTATC TGCGAAGAGA GTGGGAAACC CTTCACAACT TCCCCAAAGT CTTCATCTTA
    CCTGGTACGC CATTAAGTCG AGCTGATTTA AGGGCCGTCA ACATCAACCT CTGCGACATG
    TGCGTTATCC TGTCAGCCAA TCAGAACAAT ATCGACGATG CTTCACTGCA GGACAAGGAA
    TGCATCTTGG CATCTCTTAA CATCAAATCT ATGCAGTTTG ATGACAGCAT TGGGGTCTTG
    CAGGCTAATT CCCAAGGTTT CACACCTCCA GGGATGGATA GGTCATCTCC TGATAACAGT
    CCCGTGCACG GCTTAGTGCG GCAGGCATCC GTCACCACCG GATCAAACAT CCCCATCATA
    ACTGAACTGG TGAACGACTC CAACGTTCAG TTTTTGGACC AAGACGACGA TGACGACCCA
    GACACTGAGC TCTACCTGAC CCAGCCGTTC GCCTGTGGTA CCGCATTCGC CGTCAGCGTG
    TTGGACTCCC TGATGAGTGC GACATACTTC AATGATAATA TCCTCACCCT GATCCGGACG
    CTGGTCACGG GAGGCGCCAC GCCGGAGCTG GAGGCCCTGT TGGCTGAGGA GAACGCTCTG
    AGAGGTGGCT ACAGCACGCC GCAGACGCTG GCTAACAGGG ACAGGTGTCG GGTCGCCCAG
    CTGGCCCTCT ACGATGGGCC CTTCGCAGAC TTGGGGGACG GAGGTTGCTA TGGAGACTTG
    TTCTGTAAAG CATTAAAAAC CTACAACATG CTGTGCTTTG GAATCTATAG ATTGCGAGAT
    GCACATTTAG GTGCACCAAG CCAGTGCACA AAAAGATATG TCATCACAAA CCCGCCTTAT
    GAGTTTGAGA TGGTGCCCAC GGACCTGATC TTCTGTCTGA TGCAGTTCGA CCACAACGCG
    GGCCAGTCCC GAGCGAGCCT GTCCCACTCC TCCCATTCGT CCCATTCCTC CAGCAAGAAG
    AGCTCGTCTG TTCACTCCAT CCCAGCCACC AACCGGCAGA ACCGCAGCAG CAAAGCACGT
    GAAGCCCGTG ACAAACAGAA TGCAACAAGG ATGAATAGAA TGGGCCCAGA AAAGAGATGG
    TTTACAGATG AAGCAGAAAA CGCATACCCA AGGAACATTC AAATCAAGCC TATGAGCACT
    CACATGGCCA ACCAAGTCAA TCAGTACAAA TCCACTAGCA GTCTGATTCC ACCTATCCGA
    GAAGTCGAAG ACGAATGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156715.1
    CDS676..4476
    Translation

    Target ORF information:

    RefSeq Version XM_005156658.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156658.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT TCGAAGATGA GCATCCGTCA
    ACACTGTCAC CCAAAAAGAA GCAGCGCAAC GGAGGCATGC GAAATTCACC CAACTGCTCG
    CCCAAAATGA TGAGGCATGA TCCTCTACTC ATTCCAGGAA ATGAGCAGAT TGAGAGCATG
    GATGCTAATG TCAAACGATA CGACTCTACT GGAATGTTTC ACTGGTGTCC ATCCAAAGAA
    ATCGAGAAGG TCATTCTGAC ACGCAGCGAG GCCTCAATGA CGGTTTTAAG TGGACATGTC
    GTAGTCTGCA TATTTGGAGA TGTCACGTCA GCTTTAGTGG GGCTTCGAAA CTTAGTCATG
    CCTTTGAGAG CAAGCAACTT CCATTACCAT GAGCTAAAGC CCATTGTGTT TGTGGGCTCG
    TTAGATTATC TGCGAAGAGA GTGGGAAACC CTTCACAACT TCCCCAAAGT CTTCATCTTA
    CCTGGTACGC CATTAAGTCG AGCTGATTTA AGGGCCGTCA ACATCAACCT CTGCGACATG
    TGCGTTATCC TGTCAGCCAA TCAGAACAAT ATCGACGATG CTTCACTGCA GGACAAGGAA
    TGCATCTTGG CATCTCTTAA CATCAAATCT ATGCAGTTTG ATGACAGCAT TGGGGTCTTG
    CAGGCTAATT CCCAAGGTTT CACACCTCCA GGGATGGATA GGTCATCTCC TGATAACAGT
    CCCGTGCACG GCTTAGTGCG GCAGGCATCC GTCACCACCG GATCAAACAT CCCCATCATA
    ACTGAACTGG TGAACGACTC CAACGTTCAG TTTTTGGACC AAGACGACGA TGACGACCCA
    GACACTGAGC TCTACCTGAC CCAGCCGTTC GCCTGTGGTA CCGCATTCGC CGTCAGCGTG
    TTGGACTCCC TGATGAGTGC GACATACTTC AATGATAATA TCCTCACCCT GATCCGGACG
    CTGGTCACGG GAGGCGCCAC GCCGGAGCTG GAGGCCCTGT TGGCTGAGGA GAACGCTCTG
    AGAGGTGGCT ACAGCACGCC GCAGACGCTG GCTAACAGGG ACAGGTGTCG GGTCGCCCAG
    CTGGCCCTCT ACGATGGGCC CTTCGCAGAC TTGGGGGACG GAGGTTGCTA TGGAGACTTG
    TTCTGTAAAG CATTAAAAAC CTACAACATG CTGTGCTTTG GAATCTATAG ATTGCGAGAT
    GCACATTTAG GTGCACCAAG CCAGTGCACA AAAAGATATG TCATCACAAA CCCGCCTTAT
    GAGTTTGAGA TGGTGCCCAC GGACCTGATC TTCTGTCTGA TGCAGTTCGA CCACAACGCG
    GGCCAGTCCC GAGCGAGCCT GTCCCACTCC TCCCATTCGT CCCATTCCTC CAGCAAGAAG
    AGCTCGTCTG TTCACTCCAT CCCAGCCACC AACCGGCAGA ACCGCAGCAG CAAAGCACGT
    GAAGCCCGTG ACAAACAGAA TGCAACAAGG ATGAATAGAA TGGGCCCAGA AAAGAGATGG
    TTTACAGATG AAGCAGAAAA CGCATACCCA AGGAACATTC AAATCAAGCC TATGAGCACT
    CACATGGCCA ACCAAGTCAA TCAGTACAAA TCCACTAGCA GTCTGATTCC ACCTATCCGA
    GAAGTCGAAG ACGAATGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18612
    Clone ID Related Accession (Same CDS sequence) XM_005156659.4 , XM_005156659.3
    Accession Version XM_005156659.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3780bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156659.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAACCAAG ATGTCCGTCT ACAAGCGAAT GAAACTGGCA
    TGTTGTTTTG ATTGCGGGCG CTCTGAGCGC GACTGCTCGT GCATGTCAGG CAGTGTACAT
    AGTAACATGG ACAGCCTTCA GAGGGCCTTC CCACTTTCTT CTGTCTCTGT TCATGATTGC
    TCAACCAGTT TACGTGCCTT CGAAGATGAG CATCCGTCAA CACTGTCACC CAAAAAGAAG
    CAGCGCAACG GAGGCATGCG AAATTCACCC AACTGCTCGC CCAAAATGAT GAGGCATGAT
    CCTCTACTCA TTCCAGGAAA TGAGCAGATT GAGAGCATGG ATGCTAATGT CAAACGATAC
    GACTCTACTG GAATGTTTCA CTGGTGTCCA TCCAAAGAAA TCGAGAAGGT CATTCTGACA
    CGCAGCGAGG CCTCAATGAC GGTTTTAAGT GGACATGTCG TAGTCTGCAT ATTTGGAGAT
    GTCACGTCAG CTTTAGTGGG GCTTCGAAAC TTAGTCATGC CTTTGAGAGC AAGCAACTTC
    CATTACCATG AGCTAAAGCC CATTGTGTTT GTGGGCTCGT TAGATTATCT GCGAAGAGAG
    TGGGAAACCC TTCACAACTT CCCCAAAGTC TTCATCTTAC CTGGTACGCC ATTAAGTCGA
    GCTGATTTAA GGGCCGTCAA CATCAACCTC TGCGACATGT GCGTTATCCT GTCAGCCAAT
    CAGAACAATA TCGACGATGC TTCACTGCAG GACAAGGAAT GCATCTTGGC ATCTCTTAAC
    ATCAAATCTA TGCAGTTTGA TGACAGCATT GGGGTCTTGC AGGCTAATTC CCAAGGTTTC
    ACACCTCCAG GGATGGATAG GTCATCTCCT GATAACAGTC CCGTGCACGG CTTAGTGCGG
    CAGGCATCCG TCACCACCGG ATCAAACATC CCCATCATAA CTGAACTGGT GAACGACTCC
    AACGTTCAGT TTTTGGACCA AGACGACGAT GACGACCCAG ACACTGAGCT CTACCTGACC
    CAGCCGTTCG CCTGTGGTAC CGCATTCGCC GTCAGCGTGT TGGACTCCCT GATGAGTGCG
    ACATACTTCA ATGATAATAT CCTCACCCTG ATCCGGACGC TGGTCACGGG AGGCGCCACG
    CCGGAGCTGG AGGCCCTGTT GGCTGAGGAG AACGCTCTGA GAGGTGGCTA CAGCACGCCG
    CAGACGCTGG CTAACAGGGA CAGGTGTCGG GTCGCCCAGC TGGCCCTCTA CGATGGGCCC
    TTCGCAGACT TGGGGGACGG AGGTTGCTAT GGAGACTTGT TCTGTAAAGC ATTAAAAACC
    TACAACATGC TGTGCTTTGG AATCTATAGA TTGCGAGATG CACATTTAGG TGCACCAAGC
    CAGTGCACAA AAAGATATGT CATCACAAAC CCGCCTTATG AGTTTGAGAT GGTGCCCACG
    GACCTGATCT TCTGTCTGAT GCAGTTCGAC CACAACGCGG GCCAGTCCCG AGCGAGCCTG
    TCCCACTCCT CCCATTCGTC CCATTCCTCC AGCAAGAAGA GCTCGTCTGT TCACTCCATC
    CCAGCCACCA ACCGGCAGAA CCGCAGCAGC AAAGCACGTG AAGCCCGTGA CAAACAGAAT
    GCAACAAGGA TGAATAGAAT GGGCCCAGAA AAGAGATGGT TTACAGATGA AGCAGAAAAC
    GCATACCCAA GGAACATTCA AATCAAGCCT ATGAGCACTC ACATGGCCAA CCAAGTCAAT
    CAGTACAAAT CCACTAGCAG TCTGATTCCA CCTATCCGAG AAGTCGAAGA CGAATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156716.1
    CDS677..4456
    Translation

    Target ORF information:

    RefSeq Version XM_005156659.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156659.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAACCAAG ATGTCCGTCT ACAAGCGAAT GAAACTGGCA
    TGTTGTTTTG ATTGCGGGCG CTCTGAGCGC GACTGCTCGT GCATGTCAGG CAGTGTACAT
    AGTAACATGG ACAGCCTTCA GAGGGCCTTC CCACTTTCTT CTGTCTCTGT TCATGATTGC
    TCAACCAGTT TACGTGCCTT CGAAGATGAG CATCCGTCAA CACTGTCACC CAAAAAGAAG
    CAGCGCAACG GAGGCATGCG AAATTCACCC AACTGCTCGC CCAAAATGAT GAGGCATGAT
    CCTCTACTCA TTCCAGGAAA TGAGCAGATT GAGAGCATGG ATGCTAATGT CAAACGATAC
    GACTCTACTG GAATGTTTCA CTGGTGTCCA TCCAAAGAAA TCGAGAAGGT CATTCTGACA
    CGCAGCGAGG CCTCAATGAC GGTTTTAAGT GGACATGTCG TAGTCTGCAT ATTTGGAGAT
    GTCACGTCAG CTTTAGTGGG GCTTCGAAAC TTAGTCATGC CTTTGAGAGC AAGCAACTTC
    CATTACCATG AGCTAAAGCC CATTGTGTTT GTGGGCTCGT TAGATTATCT GCGAAGAGAG
    TGGGAAACCC TTCACAACTT CCCCAAAGTC TTCATCTTAC CTGGTACGCC ATTAAGTCGA
    GCTGATTTAA GGGCCGTCAA CATCAACCTC TGCGACATGT GCGTTATCCT GTCAGCCAAT
    CAGAACAATA TCGACGATGC TTCACTGCAG GACAAGGAAT GCATCTTGGC ATCTCTTAAC
    ATCAAATCTA TGCAGTTTGA TGACAGCATT GGGGTCTTGC AGGCTAATTC CCAAGGTTTC
    ACACCTCCAG GGATGGATAG GTCATCTCCT GATAACAGTC CCGTGCACGG CTTAGTGCGG
    CAGGCATCCG TCACCACCGG ATCAAACATC CCCATCATAA CTGAACTGGT GAACGACTCC
    AACGTTCAGT TTTTGGACCA AGACGACGAT GACGACCCAG ACACTGAGCT CTACCTGACC
    CAGCCGTTCG CCTGTGGTAC CGCATTCGCC GTCAGCGTGT TGGACTCCCT GATGAGTGCG
    ACATACTTCA ATGATAATAT CCTCACCCTG ATCCGGACGC TGGTCACGGG AGGCGCCACG
    CCGGAGCTGG AGGCCCTGTT GGCTGAGGAG AACGCTCTGA GAGGTGGCTA CAGCACGCCG
    CAGACGCTGG CTAACAGGGA CAGGTGTCGG GTCGCCCAGC TGGCCCTCTA CGATGGGCCC
    TTCGCAGACT TGGGGGACGG AGGTTGCTAT GGAGACTTGT TCTGTAAAGC ATTAAAAACC
    TACAACATGC TGTGCTTTGG AATCTATAGA TTGCGAGATG CACATTTAGG TGCACCAAGC
    CAGTGCACAA AAAGATATGT CATCACAAAC CCGCCTTATG AGTTTGAGAT GGTGCCCACG
    GACCTGATCT TCTGTCTGAT GCAGTTCGAC CACAACGCGG GCCAGTCCCG AGCGAGCCTG
    TCCCACTCCT CCCATTCGTC CCATTCCTCC AGCAAGAAGA GCTCGTCTGT TCACTCCATC
    CCAGCCACCA ACCGGCAGAA CCGCAGCAGC AAAGCACGTG AAGCCCGTGA CAAACAGAAT
    GCAACAAGGA TGAATAGAAT GGGCCCAGAA AAGAGATGGT TTACAGATGA AGCAGAAAAC
    GCATACCCAA GGAACATTCA AATCAAGCCT ATGAGCACTC ACATGGCCAA CCAAGTCAAT
    CAGTACAAAT CCACTAGCAG TCTGATTCCA CCTATCCGAG AAGTCGAAGA CGAATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18612
    Clone ID Related Accession (Same CDS sequence) XM_005156659.4 , XM_005156659.3
    Accession Version XM_005156659.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3780bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156659.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAACCAAG ATGTCCGTCT ACAAGCGAAT GAAACTGGCA
    TGTTGTTTTG ATTGCGGGCG CTCTGAGCGC GACTGCTCGT GCATGTCAGG CAGTGTACAT
    AGTAACATGG ACAGCCTTCA GAGGGCCTTC CCACTTTCTT CTGTCTCTGT TCATGATTGC
    TCAACCAGTT TACGTGCCTT CGAAGATGAG CATCCGTCAA CACTGTCACC CAAAAAGAAG
    CAGCGCAACG GAGGCATGCG AAATTCACCC AACTGCTCGC CCAAAATGAT GAGGCATGAT
    CCTCTACTCA TTCCAGGAAA TGAGCAGATT GAGAGCATGG ATGCTAATGT CAAACGATAC
    GACTCTACTG GAATGTTTCA CTGGTGTCCA TCCAAAGAAA TCGAGAAGGT CATTCTGACA
    CGCAGCGAGG CCTCAATGAC GGTTTTAAGT GGACATGTCG TAGTCTGCAT ATTTGGAGAT
    GTCACGTCAG CTTTAGTGGG GCTTCGAAAC TTAGTCATGC CTTTGAGAGC AAGCAACTTC
    CATTACCATG AGCTAAAGCC CATTGTGTTT GTGGGCTCGT TAGATTATCT GCGAAGAGAG
    TGGGAAACCC TTCACAACTT CCCCAAAGTC TTCATCTTAC CTGGTACGCC ATTAAGTCGA
    GCTGATTTAA GGGCCGTCAA CATCAACCTC TGCGACATGT GCGTTATCCT GTCAGCCAAT
    CAGAACAATA TCGACGATGC TTCACTGCAG GACAAGGAAT GCATCTTGGC ATCTCTTAAC
    ATCAAATCTA TGCAGTTTGA TGACAGCATT GGGGTCTTGC AGGCTAATTC CCAAGGTTTC
    ACACCTCCAG GGATGGATAG GTCATCTCCT GATAACAGTC CCGTGCACGG CTTAGTGCGG
    CAGGCATCCG TCACCACCGG ATCAAACATC CCCATCATAA CTGAACTGGT GAACGACTCC
    AACGTTCAGT TTTTGGACCA AGACGACGAT GACGACCCAG ACACTGAGCT CTACCTGACC
    CAGCCGTTCG CCTGTGGTAC CGCATTCGCC GTCAGCGTGT TGGACTCCCT GATGAGTGCG
    ACATACTTCA ATGATAATAT CCTCACCCTG ATCCGGACGC TGGTCACGGG AGGCGCCACG
    CCGGAGCTGG AGGCCCTGTT GGCTGAGGAG AACGCTCTGA GAGGTGGCTA CAGCACGCCG
    CAGACGCTGG CTAACAGGGA CAGGTGTCGG GTCGCCCAGC TGGCCCTCTA CGATGGGCCC
    TTCGCAGACT TGGGGGACGG AGGTTGCTAT GGAGACTTGT TCTGTAAAGC ATTAAAAACC
    TACAACATGC TGTGCTTTGG AATCTATAGA TTGCGAGATG CACATTTAGG TGCACCAAGC
    CAGTGCACAA AAAGATATGT CATCACAAAC CCGCCTTATG AGTTTGAGAT GGTGCCCACG
    GACCTGATCT TCTGTCTGAT GCAGTTCGAC CACAACGCGG GCCAGTCCCG AGCGAGCCTG
    TCCCACTCCT CCCATTCGTC CCATTCCTCC AGCAAGAAGA GCTCGTCTGT TCACTCCATC
    CCAGCCACCA ACCGGCAGAA CCGCAGCAGC AAAGCACGTG AAGCCCGTGA CAAACAGAAT
    GCAACAAGGA TGAATAGAAT GGGCCCAGAA AAGAGATGGT TTACAGATGA AGCAGAAAAC
    GCATACCCAA GGAACATTCA AATCAAGCCT ATGAGCACTC ACATGGCCAA CCAAGTCAAT
    CAGTACAAAT CCACTAGCAG TCTGATTCCA CCTATCCGAG AAGTCGAAGA CGAATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156716.1
    CDS655..4434
    Translation

    Target ORF information:

    RefSeq Version XM_005156659.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156659.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAACCAAG ATGTCCGTCT ACAAGCGAAT GAAACTGGCA
    TGTTGTTTTG ATTGCGGGCG CTCTGAGCGC GACTGCTCGT GCATGTCAGG CAGTGTACAT
    AGTAACATGG ACAGCCTTCA GAGGGCCTTC CCACTTTCTT CTGTCTCTGT TCATGATTGC
    TCAACCAGTT TACGTGCCTT CGAAGATGAG CATCCGTCAA CACTGTCACC CAAAAAGAAG
    CAGCGCAACG GAGGCATGCG AAATTCACCC AACTGCTCGC CCAAAATGAT GAGGCATGAT
    CCTCTACTCA TTCCAGGAAA TGAGCAGATT GAGAGCATGG ATGCTAATGT CAAACGATAC
    GACTCTACTG GAATGTTTCA CTGGTGTCCA TCCAAAGAAA TCGAGAAGGT CATTCTGACA
    CGCAGCGAGG CCTCAATGAC GGTTTTAAGT GGACATGTCG TAGTCTGCAT ATTTGGAGAT
    GTCACGTCAG CTTTAGTGGG GCTTCGAAAC TTAGTCATGC CTTTGAGAGC AAGCAACTTC
    CATTACCATG AGCTAAAGCC CATTGTGTTT GTGGGCTCGT TAGATTATCT GCGAAGAGAG
    TGGGAAACCC TTCACAACTT CCCCAAAGTC TTCATCTTAC CTGGTACGCC ATTAAGTCGA
    GCTGATTTAA GGGCCGTCAA CATCAACCTC TGCGACATGT GCGTTATCCT GTCAGCCAAT
    CAGAACAATA TCGACGATGC TTCACTGCAG GACAAGGAAT GCATCTTGGC ATCTCTTAAC
    ATCAAATCTA TGCAGTTTGA TGACAGCATT GGGGTCTTGC AGGCTAATTC CCAAGGTTTC
    ACACCTCCAG GGATGGATAG GTCATCTCCT GATAACAGTC CCGTGCACGG CTTAGTGCGG
    CAGGCATCCG TCACCACCGG ATCAAACATC CCCATCATAA CTGAACTGGT GAACGACTCC
    AACGTTCAGT TTTTGGACCA AGACGACGAT GACGACCCAG ACACTGAGCT CTACCTGACC
    CAGCCGTTCG CCTGTGGTAC CGCATTCGCC GTCAGCGTGT TGGACTCCCT GATGAGTGCG
    ACATACTTCA ATGATAATAT CCTCACCCTG ATCCGGACGC TGGTCACGGG AGGCGCCACG
    CCGGAGCTGG AGGCCCTGTT GGCTGAGGAG AACGCTCTGA GAGGTGGCTA CAGCACGCCG
    CAGACGCTGG CTAACAGGGA CAGGTGTCGG GTCGCCCAGC TGGCCCTCTA CGATGGGCCC
    TTCGCAGACT TGGGGGACGG AGGTTGCTAT GGAGACTTGT TCTGTAAAGC ATTAAAAACC
    TACAACATGC TGTGCTTTGG AATCTATAGA TTGCGAGATG CACATTTAGG TGCACCAAGC
    CAGTGCACAA AAAGATATGT CATCACAAAC CCGCCTTATG AGTTTGAGAT GGTGCCCACG
    GACCTGATCT TCTGTCTGAT GCAGTTCGAC CACAACGCGG GCCAGTCCCG AGCGAGCCTG
    TCCCACTCCT CCCATTCGTC CCATTCCTCC AGCAAGAAGA GCTCGTCTGT TCACTCCATC
    CCAGCCACCA ACCGGCAGAA CCGCAGCAGC AAAGCACGTG AAGCCCGTGA CAAACAGAAT
    GCAACAAGGA TGAATAGAAT GGGCCCAGAA AAGAGATGGT TTACAGATGA AGCAGAAAAC
    GCATACCCAA GGAACATTCA AATCAAGCCT ATGAGCACTC ACATGGCCAA CCAAGTCAAT
    CAGTACAAAT CCACTAGCAG TCTGATTCCA CCTATCCGAG AAGTCGAAGA CGAATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18615
    Clone ID Related Accession (Same CDS sequence) XM_005156662.3 , XM_005156662.4
    Accession Version XM_005156662.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X11
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156662.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTTCGA AGATGAGCAT
    CCGTCAACAC TGTCACCCAA AAAGAAGCAG CGCAACGGAG GCATGCGAAA TTCACCCAAC
    TGCTCGCCCA AAATGATGAG GCATGATCCT CTACTCATTC CAGGAAATGA GCAGATTGAG
    AGCATGGATG CTAATGTCAA ACGATACGAC TCTACTGGAA TGTTTCACTG GTGTCCATCC
    AAAGAAATCG AGAAGGTCAT TCTGACACGC AGCGAGGCCT CAATGACGGT TTTAAGTGGA
    CATGTCGTAG TCTGCATATT TGGAGATGTC ACGTCAGCTT TAGTGGGGCT TCGAAACTTA
    GTCATGCCTT TGAGAGCAAG CAACTTCCAT TACCATGAGC TAAAGCCCAT TGTGTTTGTG
    GGCTCGTTAG ATTATCTGCG AAGAGAGTGG GAAACCCTTC ACAACTTCCC CAAAGTCTTC
    ATCTTACCTG GTACGCCATT AAGTCGAGCT GATTTAAGGG CCGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AACAATATCG ACGATGCTTC ACTGCAGGAC
    AAGGAATGCA TCTTGGCATC TCTTAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGTTTCACA CCTCCAGGGA TGGATAGGTC ATCTCCTGAT
    AACAGTCCCG TGCACGGCTT AGTGCGGCAG GCATCCGTCA CCACCGGATC AAACATCCCC
    ATCATAACTG AACTGGCTAA GCCTGGCAAA CTACTGCCTT TGGTTTCATT CAGTCAGGAA
    AAAAACAGTG GAACCAACAT ACAAATGATA ACAGAACTGG TGAACGACTC CAACGTTCAG
    TTTTTGGACC AAGACGACGA TGACGACCCA GACACTGAGC TCTACCTGAC CCAGCCGTTC
    GCCTGTGGTA CCGCATTCGC CGTCAGCGTG TTGGACTCCC TGATGAGTGC GACATACTTC
    AATGATAATA TCCTCACCCT GATCCGGACG CTGGTCACGG GAGGCGCCAC GCCGGAGCTG
    GAGGCCCTGT TGGCTGAGGA GAACGCTCTG AGAGGTGGCT ACAGCACGCC GCAGACGCTG
    GCTAACAGGG ACAGGTGTCG GGTCGCCCAG CTGGCCCTCT ACGATGGGCC CTTCGCAGAC
    TTGGGGGACG GAGGTTGCTA TGGAGACTTG TTCTGTAAAG CATTAAAAAC CTACAACATG
    CTGTGCTTTG GAATCTATAG ATTGCGAGAT GCACATTTAG GTGCACCAAG CCAGTGCACA
    AAAAGATATG TCATCACAAA CCCGCCTTAT GAGTTTGAGA TGGTGCCCAC GGACCTGATC
    TTCTGTCTGA TGCAGTTCGA CCACAACGCG GGCCAGTCCC GAGCGAGCCT GTCCCACTCC
    TCCCATTCGT CCCATTCCTC CAGCAAGAAG AGCTCGTCTG TTCACTCCAT CCCAGCCACC
    AACCGGCAGA ACCGCAGCAG CAAAGCACGT GAAGCCCGTG ACAAACAGAA TGCAACAAGG
    ATGAATAGAA TGGGCCCAGA AAAGAGATGG TTTACAGATG AAGCAGAAAA CGCATACCCA
    AGGAACATTC AAATCAAGCC TATGAGCACT CACATGGCCA ACCAAGTCAA TCAGTACAAA
    TCCACTAGCA GTCTGATTCC ACCTATCCGA GAAGTCGAAG ACGAATGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156719.1
    CDS658..4368
    Translation

    Target ORF information:

    RefSeq Version XM_005156662.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156662.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTTCGA AGATGAGCAT
    CCGTCAACAC TGTCACCCAA AAAGAAGCAG CGCAACGGAG GCATGCGAAA TTCACCCAAC
    TGCTCGCCCA AAATGATGAG GCATGATCCT CTACTCATTC CAGGAAATGA GCAGATTGAG
    AGCATGGATG CTAATGTCAA ACGATACGAC TCTACTGGAA TGTTTCACTG GTGTCCATCC
    AAAGAAATCG AGAAGGTCAT TCTGACACGC AGCGAGGCCT CAATGACGGT TTTAAGTGGA
    CATGTCGTAG TCTGCATATT TGGAGATGTC ACGTCAGCTT TAGTGGGGCT TCGAAACTTA
    GTCATGCCTT TGAGAGCAAG CAACTTCCAT TACCATGAGC TAAAGCCCAT TGTGTTTGTG
    GGCTCGTTAG ATTATCTGCG AAGAGAGTGG GAAACCCTTC ACAACTTCCC CAAAGTCTTC
    ATCTTACCTG GTACGCCATT AAGTCGAGCT GATTTAAGGG CCGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AACAATATCG ACGATGCTTC ACTGCAGGAC
    AAGGAATGCA TCTTGGCATC TCTTAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGTTTCACA CCTCCAGGGA TGGATAGGTC ATCTCCTGAT
    AACAGTCCCG TGCACGGCTT AGTGCGGCAG GCATCCGTCA CCACCGGATC AAACATCCCC
    ATCATAACTG AACTGGCTAA GCCTGGCAAA CTACTGCCTT TGGTTTCATT CAGTCAGGAA
    AAAAACAGTG GAACCAACAT ACAAATGATA ACAGAACTGG TGAACGACTC CAACGTTCAG
    TTTTTGGACC AAGACGACGA TGACGACCCA GACACTGAGC TCTACCTGAC CCAGCCGTTC
    GCCTGTGGTA CCGCATTCGC CGTCAGCGTG TTGGACTCCC TGATGAGTGC GACATACTTC
    AATGATAATA TCCTCACCCT GATCCGGACG CTGGTCACGG GAGGCGCCAC GCCGGAGCTG
    GAGGCCCTGT TGGCTGAGGA GAACGCTCTG AGAGGTGGCT ACAGCACGCC GCAGACGCTG
    GCTAACAGGG ACAGGTGTCG GGTCGCCCAG CTGGCCCTCT ACGATGGGCC CTTCGCAGAC
    TTGGGGGACG GAGGTTGCTA TGGAGACTTG TTCTGTAAAG CATTAAAAAC CTACAACATG
    CTGTGCTTTG GAATCTATAG ATTGCGAGAT GCACATTTAG GTGCACCAAG CCAGTGCACA
    AAAAGATATG TCATCACAAA CCCGCCTTAT GAGTTTGAGA TGGTGCCCAC GGACCTGATC
    TTCTGTCTGA TGCAGTTCGA CCACAACGCG GGCCAGTCCC GAGCGAGCCT GTCCCACTCC
    TCCCATTCGT CCCATTCCTC CAGCAAGAAG AGCTCGTCTG TTCACTCCAT CCCAGCCACC
    AACCGGCAGA ACCGCAGCAG CAAAGCACGT GAAGCCCGTG ACAAACAGAA TGCAACAAGG
    ATGAATAGAA TGGGCCCAGA AAAGAGATGG TTTACAGATG AAGCAGAAAA CGCATACCCA
    AGGAACATTC AAATCAAGCC TATGAGCACT CACATGGCCA ACCAAGTCAA TCAGTACAAA
    TCCACTAGCA GTCTGATTCC ACCTATCCGA GAAGTCGAAG ACGAATGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18615
    Clone ID Related Accession (Same CDS sequence) XM_005156662.3 , XM_005156662.4
    Accession Version XM_005156662.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156662.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTTCGA AGATGAGCAT
    CCGTCAACAC TGTCACCCAA AAAGAAGCAG CGCAACGGAG GCATGCGAAA TTCACCCAAC
    TGCTCGCCCA AAATGATGAG GCATGATCCT CTACTCATTC CAGGAAATGA GCAGATTGAG
    AGCATGGATG CTAATGTCAA ACGATACGAC TCTACTGGAA TGTTTCACTG GTGTCCATCC
    AAAGAAATCG AGAAGGTCAT TCTGACACGC AGCGAGGCCT CAATGACGGT TTTAAGTGGA
    CATGTCGTAG TCTGCATATT TGGAGATGTC ACGTCAGCTT TAGTGGGGCT TCGAAACTTA
    GTCATGCCTT TGAGAGCAAG CAACTTCCAT TACCATGAGC TAAAGCCCAT TGTGTTTGTG
    GGCTCGTTAG ATTATCTGCG AAGAGAGTGG GAAACCCTTC ACAACTTCCC CAAAGTCTTC
    ATCTTACCTG GTACGCCATT AAGTCGAGCT GATTTAAGGG CCGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AACAATATCG ACGATGCTTC ACTGCAGGAC
    AAGGAATGCA TCTTGGCATC TCTTAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGTTTCACA CCTCCAGGGA TGGATAGGTC ATCTCCTGAT
    AACAGTCCCG TGCACGGCTT AGTGCGGCAG GCATCCGTCA CCACCGGATC AAACATCCCC
    ATCATAACTG AACTGGCTAA GCCTGGCAAA CTACTGCCTT TGGTTTCATT CAGTCAGGAA
    AAAAACAGTG GAACCAACAT ACAAATGATA ACAGAACTGG TGAACGACTC CAACGTTCAG
    TTTTTGGACC AAGACGACGA TGACGACCCA GACACTGAGC TCTACCTGAC CCAGCCGTTC
    GCCTGTGGTA CCGCATTCGC CGTCAGCGTG TTGGACTCCC TGATGAGTGC GACATACTTC
    AATGATAATA TCCTCACCCT GATCCGGACG CTGGTCACGG GAGGCGCCAC GCCGGAGCTG
    GAGGCCCTGT TGGCTGAGGA GAACGCTCTG AGAGGTGGCT ACAGCACGCC GCAGACGCTG
    GCTAACAGGG ACAGGTGTCG GGTCGCCCAG CTGGCCCTCT ACGATGGGCC CTTCGCAGAC
    TTGGGGGACG GAGGTTGCTA TGGAGACTTG TTCTGTAAAG CATTAAAAAC CTACAACATG
    CTGTGCTTTG GAATCTATAG ATTGCGAGAT GCACATTTAG GTGCACCAAG CCAGTGCACA
    AAAAGATATG TCATCACAAA CCCGCCTTAT GAGTTTGAGA TGGTGCCCAC GGACCTGATC
    TTCTGTCTGA TGCAGTTCGA CCACAACGCG GGCCAGTCCC GAGCGAGCCT GTCCCACTCC
    TCCCATTCGT CCCATTCCTC CAGCAAGAAG AGCTCGTCTG TTCACTCCAT CCCAGCCACC
    AACCGGCAGA ACCGCAGCAG CAAAGCACGT GAAGCCCGTG ACAAACAGAA TGCAACAAGG
    ATGAATAGAA TGGGCCCAGA AAAGAGATGG TTTACAGATG AAGCAGAAAA CGCATACCCA
    AGGAACATTC AAATCAAGCC TATGAGCACT CACATGGCCA ACCAAGTCAA TCAGTACAAA
    TCCACTAGCA GTCTGATTCC ACCTATCCGA GAAGTCGAAG ACGAATGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156719.1
    CDS676..4386
    Translation

    Target ORF information:

    RefSeq Version XM_005156662.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156662.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTTCGA AGATGAGCAT
    CCGTCAACAC TGTCACCCAA AAAGAAGCAG CGCAACGGAG GCATGCGAAA TTCACCCAAC
    TGCTCGCCCA AAATGATGAG GCATGATCCT CTACTCATTC CAGGAAATGA GCAGATTGAG
    AGCATGGATG CTAATGTCAA ACGATACGAC TCTACTGGAA TGTTTCACTG GTGTCCATCC
    AAAGAAATCG AGAAGGTCAT TCTGACACGC AGCGAGGCCT CAATGACGGT TTTAAGTGGA
    CATGTCGTAG TCTGCATATT TGGAGATGTC ACGTCAGCTT TAGTGGGGCT TCGAAACTTA
    GTCATGCCTT TGAGAGCAAG CAACTTCCAT TACCATGAGC TAAAGCCCAT TGTGTTTGTG
    GGCTCGTTAG ATTATCTGCG AAGAGAGTGG GAAACCCTTC ACAACTTCCC CAAAGTCTTC
    ATCTTACCTG GTACGCCATT AAGTCGAGCT GATTTAAGGG CCGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AACAATATCG ACGATGCTTC ACTGCAGGAC
    AAGGAATGCA TCTTGGCATC TCTTAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGTTTCACA CCTCCAGGGA TGGATAGGTC ATCTCCTGAT
    AACAGTCCCG TGCACGGCTT AGTGCGGCAG GCATCCGTCA CCACCGGATC AAACATCCCC
    ATCATAACTG AACTGGCTAA GCCTGGCAAA CTACTGCCTT TGGTTTCATT CAGTCAGGAA
    AAAAACAGTG GAACCAACAT ACAAATGATA ACAGAACTGG TGAACGACTC CAACGTTCAG
    TTTTTGGACC AAGACGACGA TGACGACCCA GACACTGAGC TCTACCTGAC CCAGCCGTTC
    GCCTGTGGTA CCGCATTCGC CGTCAGCGTG TTGGACTCCC TGATGAGTGC GACATACTTC
    AATGATAATA TCCTCACCCT GATCCGGACG CTGGTCACGG GAGGCGCCAC GCCGGAGCTG
    GAGGCCCTGT TGGCTGAGGA GAACGCTCTG AGAGGTGGCT ACAGCACGCC GCAGACGCTG
    GCTAACAGGG ACAGGTGTCG GGTCGCCCAG CTGGCCCTCT ACGATGGGCC CTTCGCAGAC
    TTGGGGGACG GAGGTTGCTA TGGAGACTTG TTCTGTAAAG CATTAAAAAC CTACAACATG
    CTGTGCTTTG GAATCTATAG ATTGCGAGAT GCACATTTAG GTGCACCAAG CCAGTGCACA
    AAAAGATATG TCATCACAAA CCCGCCTTAT GAGTTTGAGA TGGTGCCCAC GGACCTGATC
    TTCTGTCTGA TGCAGTTCGA CCACAACGCG GGCCAGTCCC GAGCGAGCCT GTCCCACTCC
    TCCCATTCGT CCCATTCCTC CAGCAAGAAG AGCTCGTCTG TTCACTCCAT CCCAGCCACC
    AACCGGCAGA ACCGCAGCAG CAAAGCACGT GAAGCCCGTG ACAAACAGAA TGCAACAAGG
    ATGAATAGAA TGGGCCCAGA AAAGAGATGG TTTACAGATG AAGCAGAAAA CGCATACCCA
    AGGAACATTC AAATCAAGCC TATGAGCACT CACATGGCCA ACCAAGTCAA TCAGTACAAA
    TCCACTAGCA GTCTGATTCC ACCTATCCGA GAAGTCGAAG ACGAATGCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18616
    Clone ID Related Accession (Same CDS sequence) XM_005156663.4 , XM_005156663.3
    Accession Version XM_005156663.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3702bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X14
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156663.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATCG AAGATGAGCA TCCGTCAACA
    CTGTCACCCA AAAAGAAGCA GCGCAACGGA GGCATGCGAA ATTCACCCAA CTGCTCGCCC
    AAAATGATGA GGCATGATCC TCTACTCATT CCAGGAAATG AGCAGATTGA GAGCATGGAT
    GCTAATGTCA AACGATACGA CTCTACTGGA ATGTTTCACT GGTGTCCATC CAAAGAAATC
    GAGAAGGTCA TTCTGACACG CAGCGAGGCC TCAATGACGG TTTTAAGTGG ACATGTCGTA
    GTCTGCATAT TTGGAGATGT CACGTCAGCT TTAGTGGGGC TTCGAAACTT AGTCATGCCT
    TTGAGAGCAA GCAACTTCCA TTACCATGAG CTAAAGCCCA TTGTGTTTGT GGGCTCGTTA
    GATTATCTGC GAAGAGAGTG GGAAACCCTT CACAACTTCC CCAAAGTCTT CATCTTACCT
    GGTACGCCAT TAAGTCGAGC TGATTTAAGG GCCGTCAACA TCAACCTCTG CGACATGTGC
    GTTATCCTGT CAGCCAATCA GAACAATATC GACGATGCTT CACTGCAGGA CAAGGAATGC
    ATCTTGGCAT CTCTTAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG
    GCTAATTCCC AAGGTTTCAC ACCTCCAGGG ATGGATAGGT CATCTCCTGA TAACAGTCCC
    GTGCACGGCT TAGTGCGGCA GGCATCCGTC ACCACCGGAT CAAACATCCC CATCATAACT
    GAACTGGCTA AGCCTGGCAA ACTACTGCCT TTGGTTTCAT TCAGTCAGGA AAAAAACAGT
    GGAACCAACA TACAAATGAT AACAGAACTG GTGAACGACT CCAACGTTCA GTTTTTGGAC
    CAAGACGACG ATGACGACCC AGACACTGAG CTCTACCTGA CCCAGCCGTT CGCCTGTGGT
    ACCGCATTCG CCGTCAGCGT GTTGGACTCC CTGATGAGTG CGACATACTT CAATGATAAT
    ATCCTCACCC TGATCCGGAC GCTGGTCACG GGAGGCGCCA CGCCGGAGCT GGAGGCCCTG
    TTGGCTGAGG AGAACGCTCT GAGAGGTGGC TACAGCACGC CGCAGACGCT GGCTAACAGG
    GACAGGTGTC GGGTCGCCCA GCTGGCCCTC TACGATGGGC CCTTCGCAGA CTTGGGGGAC
    GGAGGTTGCT ATGGAGACTT GTTCTGTAAA GCATTAAAAA CCTACAACAT GCTGTGCTTT
    GGAATCTATA GATTGCGAGA TGCACATTTA GGTGCACCAA GCCAGTGCAC AAAAAGATAT
    GTCATCACAA ACCCGCCTTA TGAGTTTGAG ATGGTGCCCA CGGACCTGAT CTTCTGTCTG
    ATGCAGTTCG ACCACAACGC GGGCCAGTCC CGAGCGAGCC TGTCCCACTC CTCCCATTCG
    TCCCATTCCT CCAGCAAGAA GAGCTCGTCT GTTCACTCCA TCCCAGCCAC CAACCGGCAG
    AACCGCAGCA GCAAAGCACG TGAAGCCCGT GACAAACAGA ATGCAACAAG GATGAATAGA
    ATGGGCCCAG AAAAGAGATG GTTTACAGAT GAAGCAGAAA ACGCATACCC AAGGAACATT
    CAAATCAAGC CTATGAGCAC TCACATGGCC AACCAAGTCA ATCAGTACAA ATCCACTAGC
    AGTCTGATTC CACCTATCCG AGAAGTCGAA GACGAATGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156720.1
    CDS676..4377
    Translation

    Target ORF information:

    RefSeq Version XM_005156663.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156663.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATCG AAGATGAGCA TCCGTCAACA
    CTGTCACCCA AAAAGAAGCA GCGCAACGGA GGCATGCGAA ATTCACCCAA CTGCTCGCCC
    AAAATGATGA GGCATGATCC TCTACTCATT CCAGGAAATG AGCAGATTGA GAGCATGGAT
    GCTAATGTCA AACGATACGA CTCTACTGGA ATGTTTCACT GGTGTCCATC CAAAGAAATC
    GAGAAGGTCA TTCTGACACG CAGCGAGGCC TCAATGACGG TTTTAAGTGG ACATGTCGTA
    GTCTGCATAT TTGGAGATGT CACGTCAGCT TTAGTGGGGC TTCGAAACTT AGTCATGCCT
    TTGAGAGCAA GCAACTTCCA TTACCATGAG CTAAAGCCCA TTGTGTTTGT GGGCTCGTTA
    GATTATCTGC GAAGAGAGTG GGAAACCCTT CACAACTTCC CCAAAGTCTT CATCTTACCT
    GGTACGCCAT TAAGTCGAGC TGATTTAAGG GCCGTCAACA TCAACCTCTG CGACATGTGC
    GTTATCCTGT CAGCCAATCA GAACAATATC GACGATGCTT CACTGCAGGA CAAGGAATGC
    ATCTTGGCAT CTCTTAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG
    GCTAATTCCC AAGGTTTCAC ACCTCCAGGG ATGGATAGGT CATCTCCTGA TAACAGTCCC
    GTGCACGGCT TAGTGCGGCA GGCATCCGTC ACCACCGGAT CAAACATCCC CATCATAACT
    GAACTGGCTA AGCCTGGCAA ACTACTGCCT TTGGTTTCAT TCAGTCAGGA AAAAAACAGT
    GGAACCAACA TACAAATGAT AACAGAACTG GTGAACGACT CCAACGTTCA GTTTTTGGAC
    CAAGACGACG ATGACGACCC AGACACTGAG CTCTACCTGA CCCAGCCGTT CGCCTGTGGT
    ACCGCATTCG CCGTCAGCGT GTTGGACTCC CTGATGAGTG CGACATACTT CAATGATAAT
    ATCCTCACCC TGATCCGGAC GCTGGTCACG GGAGGCGCCA CGCCGGAGCT GGAGGCCCTG
    TTGGCTGAGG AGAACGCTCT GAGAGGTGGC TACAGCACGC CGCAGACGCT GGCTAACAGG
    GACAGGTGTC GGGTCGCCCA GCTGGCCCTC TACGATGGGC CCTTCGCAGA CTTGGGGGAC
    GGAGGTTGCT ATGGAGACTT GTTCTGTAAA GCATTAAAAA CCTACAACAT GCTGTGCTTT
    GGAATCTATA GATTGCGAGA TGCACATTTA GGTGCACCAA GCCAGTGCAC AAAAAGATAT
    GTCATCACAA ACCCGCCTTA TGAGTTTGAG ATGGTGCCCA CGGACCTGAT CTTCTGTCTG
    ATGCAGTTCG ACCACAACGC GGGCCAGTCC CGAGCGAGCC TGTCCCACTC CTCCCATTCG
    TCCCATTCCT CCAGCAAGAA GAGCTCGTCT GTTCACTCCA TCCCAGCCAC CAACCGGCAG
    AACCGCAGCA GCAAAGCACG TGAAGCCCGT GACAAACAGA ATGCAACAAG GATGAATAGA
    ATGGGCCCAG AAAAGAGATG GTTTACAGAT GAAGCAGAAA ACGCATACCC AAGGAACATT
    CAAATCAAGC CTATGAGCAC TCACATGGCC AACCAAGTCA ATCAGTACAA ATCCACTAGC
    AGTCTGATTC CACCTATCCG AGAAGTCGAA GACGAATGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18616
    Clone ID Related Accession (Same CDS sequence) XM_005156663.4 , XM_005156663.3
    Accession Version XM_005156663.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3702bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X12
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156663.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATCG AAGATGAGCA TCCGTCAACA
    CTGTCACCCA AAAAGAAGCA GCGCAACGGA GGCATGCGAA ATTCACCCAA CTGCTCGCCC
    AAAATGATGA GGCATGATCC TCTACTCATT CCAGGAAATG AGCAGATTGA GAGCATGGAT
    GCTAATGTCA AACGATACGA CTCTACTGGA ATGTTTCACT GGTGTCCATC CAAAGAAATC
    GAGAAGGTCA TTCTGACACG CAGCGAGGCC TCAATGACGG TTTTAAGTGG ACATGTCGTA
    GTCTGCATAT TTGGAGATGT CACGTCAGCT TTAGTGGGGC TTCGAAACTT AGTCATGCCT
    TTGAGAGCAA GCAACTTCCA TTACCATGAG CTAAAGCCCA TTGTGTTTGT GGGCTCGTTA
    GATTATCTGC GAAGAGAGTG GGAAACCCTT CACAACTTCC CCAAAGTCTT CATCTTACCT
    GGTACGCCAT TAAGTCGAGC TGATTTAAGG GCCGTCAACA TCAACCTCTG CGACATGTGC
    GTTATCCTGT CAGCCAATCA GAACAATATC GACGATGCTT CACTGCAGGA CAAGGAATGC
    ATCTTGGCAT CTCTTAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG
    GCTAATTCCC AAGGTTTCAC ACCTCCAGGG ATGGATAGGT CATCTCCTGA TAACAGTCCC
    GTGCACGGCT TAGTGCGGCA GGCATCCGTC ACCACCGGAT CAAACATCCC CATCATAACT
    GAACTGGCTA AGCCTGGCAA ACTACTGCCT TTGGTTTCAT TCAGTCAGGA AAAAAACAGT
    GGAACCAACA TACAAATGAT AACAGAACTG GTGAACGACT CCAACGTTCA GTTTTTGGAC
    CAAGACGACG ATGACGACCC AGACACTGAG CTCTACCTGA CCCAGCCGTT CGCCTGTGGT
    ACCGCATTCG CCGTCAGCGT GTTGGACTCC CTGATGAGTG CGACATACTT CAATGATAAT
    ATCCTCACCC TGATCCGGAC GCTGGTCACG GGAGGCGCCA CGCCGGAGCT GGAGGCCCTG
    TTGGCTGAGG AGAACGCTCT GAGAGGTGGC TACAGCACGC CGCAGACGCT GGCTAACAGG
    GACAGGTGTC GGGTCGCCCA GCTGGCCCTC TACGATGGGC CCTTCGCAGA CTTGGGGGAC
    GGAGGTTGCT ATGGAGACTT GTTCTGTAAA GCATTAAAAA CCTACAACAT GCTGTGCTTT
    GGAATCTATA GATTGCGAGA TGCACATTTA GGTGCACCAA GCCAGTGCAC AAAAAGATAT
    GTCATCACAA ACCCGCCTTA TGAGTTTGAG ATGGTGCCCA CGGACCTGAT CTTCTGTCTG
    ATGCAGTTCG ACCACAACGC GGGCCAGTCC CGAGCGAGCC TGTCCCACTC CTCCCATTCG
    TCCCATTCCT CCAGCAAGAA GAGCTCGTCT GTTCACTCCA TCCCAGCCAC CAACCGGCAG
    AACCGCAGCA GCAAAGCACG TGAAGCCCGT GACAAACAGA ATGCAACAAG GATGAATAGA
    ATGGGCCCAG AAAAGAGATG GTTTACAGAT GAAGCAGAAA ACGCATACCC AAGGAACATT
    CAAATCAAGC CTATGAGCAC TCACATGGCC AACCAAGTCA ATCAGTACAA ATCCACTAGC
    AGTCTGATTC CACCTATCCG AGAAGTCGAA GACGAATGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156720.1
    CDS658..4359
    Translation

    Target ORF information:

    RefSeq Version XM_005156663.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156663.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATCG AAGATGAGCA TCCGTCAACA
    CTGTCACCCA AAAAGAAGCA GCGCAACGGA GGCATGCGAA ATTCACCCAA CTGCTCGCCC
    AAAATGATGA GGCATGATCC TCTACTCATT CCAGGAAATG AGCAGATTGA GAGCATGGAT
    GCTAATGTCA AACGATACGA CTCTACTGGA ATGTTTCACT GGTGTCCATC CAAAGAAATC
    GAGAAGGTCA TTCTGACACG CAGCGAGGCC TCAATGACGG TTTTAAGTGG ACATGTCGTA
    GTCTGCATAT TTGGAGATGT CACGTCAGCT TTAGTGGGGC TTCGAAACTT AGTCATGCCT
    TTGAGAGCAA GCAACTTCCA TTACCATGAG CTAAAGCCCA TTGTGTTTGT GGGCTCGTTA
    GATTATCTGC GAAGAGAGTG GGAAACCCTT CACAACTTCC CCAAAGTCTT CATCTTACCT
    GGTACGCCAT TAAGTCGAGC TGATTTAAGG GCCGTCAACA TCAACCTCTG CGACATGTGC
    GTTATCCTGT CAGCCAATCA GAACAATATC GACGATGCTT CACTGCAGGA CAAGGAATGC
    ATCTTGGCAT CTCTTAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG
    GCTAATTCCC AAGGTTTCAC ACCTCCAGGG ATGGATAGGT CATCTCCTGA TAACAGTCCC
    GTGCACGGCT TAGTGCGGCA GGCATCCGTC ACCACCGGAT CAAACATCCC CATCATAACT
    GAACTGGCTA AGCCTGGCAA ACTACTGCCT TTGGTTTCAT TCAGTCAGGA AAAAAACAGT
    GGAACCAACA TACAAATGAT AACAGAACTG GTGAACGACT CCAACGTTCA GTTTTTGGAC
    CAAGACGACG ATGACGACCC AGACACTGAG CTCTACCTGA CCCAGCCGTT CGCCTGTGGT
    ACCGCATTCG CCGTCAGCGT GTTGGACTCC CTGATGAGTG CGACATACTT CAATGATAAT
    ATCCTCACCC TGATCCGGAC GCTGGTCACG GGAGGCGCCA CGCCGGAGCT GGAGGCCCTG
    TTGGCTGAGG AGAACGCTCT GAGAGGTGGC TACAGCACGC CGCAGACGCT GGCTAACAGG
    GACAGGTGTC GGGTCGCCCA GCTGGCCCTC TACGATGGGC CCTTCGCAGA CTTGGGGGAC
    GGAGGTTGCT ATGGAGACTT GTTCTGTAAA GCATTAAAAA CCTACAACAT GCTGTGCTTT
    GGAATCTATA GATTGCGAGA TGCACATTTA GGTGCACCAA GCCAGTGCAC AAAAAGATAT
    GTCATCACAA ACCCGCCTTA TGAGTTTGAG ATGGTGCCCA CGGACCTGAT CTTCTGTCTG
    ATGCAGTTCG ACCACAACGC GGGCCAGTCC CGAGCGAGCC TGTCCCACTC CTCCCATTCG
    TCCCATTCCT CCAGCAAGAA GAGCTCGTCT GTTCACTCCA TCCCAGCCAC CAACCGGCAG
    AACCGCAGCA GCAAAGCACG TGAAGCCCGT GACAAACAGA ATGCAACAAG GATGAATAGA
    ATGGGCCCAG AAAAGAGATG GTTTACAGAT GAAGCAGAAA ACGCATACCC AAGGAACATT
    CAAATCAAGC CTATGAGCAC TCACATGGCC AACCAAGTCA ATCAGTACAA ATCCACTAGC
    AGTCTGATTC CACCTATCCG AGAAGTCGAA GACGAATGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18617
    Clone ID Related Accession (Same CDS sequence) XM_005156664.4 , XM_005156664.3
    Accession Version XM_005156664.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3699bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X15
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156664.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATGACA TATTTCGAAG ATGAGCATCC GTCAACACTG
    TCACCCAAAA AGAAGCAGCG CAACGGAGGC ATGCGAAATT CACCCAACTG CTCGCCCAAA
    ATGATGAGGC ATGATCCTCT ACTCATTCCA GGAAATGAGC AGATTGAGAG CATGGATGCT
    AATGTCAAAC GATACGACTC TACTGGAATG TTTCACTGGT GTCCATCCAA AGAAATCGAG
    AAGGTCATTC TGACACGCAG CGAGGCCTCA ATGACGGTTT TAAGTGGACA TGTCGTAGTC
    TGCATATTTG GAGATGTCAC GTCAGCTTTA GTGGGGCTTC GAAACTTAGT CATGCCTTTG
    AGAGCAAGCA ACTTCCATTA CCATGAGCTA AAGCCCATTG TGTTTGTGGG CTCGTTAGAT
    TATCTGCGAA GAGAGTGGGA AACCCTTCAC AACTTCCCCA AAGTCTTCAT CTTACCTGGT
    ACGCCATTAA GTCGAGCTGA TTTAAGGGCC GTCAACATCA ACCTCTGCGA CATGTGCGTT
    ATCCTGTCAG CCAATCAGAA CAATATCGAC GATGCTTCAC TGCAGGACAA GGAATGCATC
    TTGGCATCTC TTAACATCAA ATCTATGCAG TTTGATGACA GCATTGGGGT CTTGCAGGCT
    AATTCCCAAG GTTTCACACC TCCAGGGATG GATAGGTCAT CTCCTGATAA CAGTCCCGTG
    CACGGCTTAG TGCGGCAGGC ATCCGTCACC ACCGGATCAA ACATCCCCAT CATAACTGAA
    CTGGCTAAGC CTGGCAAACT ACTGCCTTTG GTTTCATTCA GTCAGGAAAA AAACAGTGGA
    ACCAACATAC AAATGATAAC AGAACTGGTG AACGACTCCA ACGTTCAGTT TTTGGACCAA
    GACGACGATG ACGACCCAGA CACTGAGCTC TACCTGACCC AGCCGTTCGC CTGTGGTACC
    GCATTCGCCG TCAGCGTGTT GGACTCCCTG ATGAGTGCGA CATACTTCAA TGATAATATC
    CTCACCCTGA TCCGGACGCT GGTCACGGGA GGCGCCACGC CGGAGCTGGA GGCCCTGTTG
    GCTGAGGAGA ACGCTCTGAG AGGTGGCTAC AGCACGCCGC AGACGCTGGC TAACAGGGAC
    AGGTGTCGGG TCGCCCAGCT GGCCCTCTAC GATGGGCCCT TCGCAGACTT GGGGGACGGA
    GGTTGCTATG GAGACTTGTT CTGTAAAGCA TTAAAAACCT ACAACATGCT GTGCTTTGGA
    ATCTATAGAT TGCGAGATGC ACATTTAGGT GCACCAAGCC AGTGCACAAA AAGATATGTC
    ATCACAAACC CGCCTTATGA GTTTGAGATG GTGCCCACGG ACCTGATCTT CTGTCTGATG
    CAGTTCGACC ACAACGCGGG CCAGTCCCGA GCGAGCCTGT CCCACTCCTC CCATTCGTCC
    CATTCCTCCA GCAAGAAGAG CTCGTCTGTT CACTCCATCC CAGCCACCAA CCGGCAGAAC
    CGCAGCAGCA AAGCACGTGA AGCCCGTGAC AAACAGAATG CAACAAGGAT GAATAGAATG
    GGCCCAGAAA AGAGATGGTT TACAGATGAA GCAGAAAACG CATACCCAAG GAACATTCAA
    ATCAAGCCTA TGAGCACTCA CATGGCCAAC CAAGTCAATC AGTACAAATC CACTAGCAGT
    CTGATTCCAC CTATCCGAGA AGTCGAAGAC GAATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156721.1
    CDS677..4375
    Translation

    Target ORF information:

    RefSeq Version XM_005156664.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156664.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATGACA TATTTCGAAG ATGAGCATCC GTCAACACTG
    TCACCCAAAA AGAAGCAGCG CAACGGAGGC ATGCGAAATT CACCCAACTG CTCGCCCAAA
    ATGATGAGGC ATGATCCTCT ACTCATTCCA GGAAATGAGC AGATTGAGAG CATGGATGCT
    AATGTCAAAC GATACGACTC TACTGGAATG TTTCACTGGT GTCCATCCAA AGAAATCGAG
    AAGGTCATTC TGACACGCAG CGAGGCCTCA ATGACGGTTT TAAGTGGACA TGTCGTAGTC
    TGCATATTTG GAGATGTCAC GTCAGCTTTA GTGGGGCTTC GAAACTTAGT CATGCCTTTG
    AGAGCAAGCA ACTTCCATTA CCATGAGCTA AAGCCCATTG TGTTTGTGGG CTCGTTAGAT
    TATCTGCGAA GAGAGTGGGA AACCCTTCAC AACTTCCCCA AAGTCTTCAT CTTACCTGGT
    ACGCCATTAA GTCGAGCTGA TTTAAGGGCC GTCAACATCA ACCTCTGCGA CATGTGCGTT
    ATCCTGTCAG CCAATCAGAA CAATATCGAC GATGCTTCAC TGCAGGACAA GGAATGCATC
    TTGGCATCTC TTAACATCAA ATCTATGCAG TTTGATGACA GCATTGGGGT CTTGCAGGCT
    AATTCCCAAG GTTTCACACC TCCAGGGATG GATAGGTCAT CTCCTGATAA CAGTCCCGTG
    CACGGCTTAG TGCGGCAGGC ATCCGTCACC ACCGGATCAA ACATCCCCAT CATAACTGAA
    CTGGCTAAGC CTGGCAAACT ACTGCCTTTG GTTTCATTCA GTCAGGAAAA AAACAGTGGA
    ACCAACATAC AAATGATAAC AGAACTGGTG AACGACTCCA ACGTTCAGTT TTTGGACCAA
    GACGACGATG ACGACCCAGA CACTGAGCTC TACCTGACCC AGCCGTTCGC CTGTGGTACC
    GCATTCGCCG TCAGCGTGTT GGACTCCCTG ATGAGTGCGA CATACTTCAA TGATAATATC
    CTCACCCTGA TCCGGACGCT GGTCACGGGA GGCGCCACGC CGGAGCTGGA GGCCCTGTTG
    GCTGAGGAGA ACGCTCTGAG AGGTGGCTAC AGCACGCCGC AGACGCTGGC TAACAGGGAC
    AGGTGTCGGG TCGCCCAGCT GGCCCTCTAC GATGGGCCCT TCGCAGACTT GGGGGACGGA
    GGTTGCTATG GAGACTTGTT CTGTAAAGCA TTAAAAACCT ACAACATGCT GTGCTTTGGA
    ATCTATAGAT TGCGAGATGC ACATTTAGGT GCACCAAGCC AGTGCACAAA AAGATATGTC
    ATCACAAACC CGCCTTATGA GTTTGAGATG GTGCCCACGG ACCTGATCTT CTGTCTGATG
    CAGTTCGACC ACAACGCGGG CCAGTCCCGA GCGAGCCTGT CCCACTCCTC CCATTCGTCC
    CATTCCTCCA GCAAGAAGAG CTCGTCTGTT CACTCCATCC CAGCCACCAA CCGGCAGAAC
    CGCAGCAGCA AAGCACGTGA AGCCCGTGAC AAACAGAATG CAACAAGGAT GAATAGAATG
    GGCCCAGAAA AGAGATGGTT TACAGATGAA GCAGAAAACG CATACCCAAG GAACATTCAA
    ATCAAGCCTA TGAGCACTCA CATGGCCAAC CAAGTCAATC AGTACAAATC CACTAGCAGT
    CTGATTCCAC CTATCCGAGA AGTCGAAGAC GAATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18617
    Clone ID Related Accession (Same CDS sequence) XM_005156664.4 , XM_005156664.3
    Accession Version XM_005156664.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3699bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X13
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156664.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATGACA TATTTCGAAG ATGAGCATCC GTCAACACTG
    TCACCCAAAA AGAAGCAGCG CAACGGAGGC ATGCGAAATT CACCCAACTG CTCGCCCAAA
    ATGATGAGGC ATGATCCTCT ACTCATTCCA GGAAATGAGC AGATTGAGAG CATGGATGCT
    AATGTCAAAC GATACGACTC TACTGGAATG TTTCACTGGT GTCCATCCAA AGAAATCGAG
    AAGGTCATTC TGACACGCAG CGAGGCCTCA ATGACGGTTT TAAGTGGACA TGTCGTAGTC
    TGCATATTTG GAGATGTCAC GTCAGCTTTA GTGGGGCTTC GAAACTTAGT CATGCCTTTG
    AGAGCAAGCA ACTTCCATTA CCATGAGCTA AAGCCCATTG TGTTTGTGGG CTCGTTAGAT
    TATCTGCGAA GAGAGTGGGA AACCCTTCAC AACTTCCCCA AAGTCTTCAT CTTACCTGGT
    ACGCCATTAA GTCGAGCTGA TTTAAGGGCC GTCAACATCA ACCTCTGCGA CATGTGCGTT
    ATCCTGTCAG CCAATCAGAA CAATATCGAC GATGCTTCAC TGCAGGACAA GGAATGCATC
    TTGGCATCTC TTAACATCAA ATCTATGCAG TTTGATGACA GCATTGGGGT CTTGCAGGCT
    AATTCCCAAG GTTTCACACC TCCAGGGATG GATAGGTCAT CTCCTGATAA CAGTCCCGTG
    CACGGCTTAG TGCGGCAGGC ATCCGTCACC ACCGGATCAA ACATCCCCAT CATAACTGAA
    CTGGCTAAGC CTGGCAAACT ACTGCCTTTG GTTTCATTCA GTCAGGAAAA AAACAGTGGA
    ACCAACATAC AAATGATAAC AGAACTGGTG AACGACTCCA ACGTTCAGTT TTTGGACCAA
    GACGACGATG ACGACCCAGA CACTGAGCTC TACCTGACCC AGCCGTTCGC CTGTGGTACC
    GCATTCGCCG TCAGCGTGTT GGACTCCCTG ATGAGTGCGA CATACTTCAA TGATAATATC
    CTCACCCTGA TCCGGACGCT GGTCACGGGA GGCGCCACGC CGGAGCTGGA GGCCCTGTTG
    GCTGAGGAGA ACGCTCTGAG AGGTGGCTAC AGCACGCCGC AGACGCTGGC TAACAGGGAC
    AGGTGTCGGG TCGCCCAGCT GGCCCTCTAC GATGGGCCCT TCGCAGACTT GGGGGACGGA
    GGTTGCTATG GAGACTTGTT CTGTAAAGCA TTAAAAACCT ACAACATGCT GTGCTTTGGA
    ATCTATAGAT TGCGAGATGC ACATTTAGGT GCACCAAGCC AGTGCACAAA AAGATATGTC
    ATCACAAACC CGCCTTATGA GTTTGAGATG GTGCCCACGG ACCTGATCTT CTGTCTGATG
    CAGTTCGACC ACAACGCGGG CCAGTCCCGA GCGAGCCTGT CCCACTCCTC CCATTCGTCC
    CATTCCTCCA GCAAGAAGAG CTCGTCTGTT CACTCCATCC CAGCCACCAA CCGGCAGAAC
    CGCAGCAGCA AAGCACGTGA AGCCCGTGAC AAACAGAATG CAACAAGGAT GAATAGAATG
    GGCCCAGAAA AGAGATGGTT TACAGATGAA GCAGAAAACG CATACCCAAG GAACATTCAA
    ATCAAGCCTA TGAGCACTCA CATGGCCAAC CAAGTCAATC AGTACAAATC CACTAGCAGT
    CTGATTCCAC CTATCCGAGA AGTCGAAGAC GAATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156721.1
    CDS660..4358
    Translation

    Target ORF information:

    RefSeq Version XM_005156664.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156664.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATGACA TATTTCGAAG ATGAGCATCC GTCAACACTG
    TCACCCAAAA AGAAGCAGCG CAACGGAGGC ATGCGAAATT CACCCAACTG CTCGCCCAAA
    ATGATGAGGC ATGATCCTCT ACTCATTCCA GGAAATGAGC AGATTGAGAG CATGGATGCT
    AATGTCAAAC GATACGACTC TACTGGAATG TTTCACTGGT GTCCATCCAA AGAAATCGAG
    AAGGTCATTC TGACACGCAG CGAGGCCTCA ATGACGGTTT TAAGTGGACA TGTCGTAGTC
    TGCATATTTG GAGATGTCAC GTCAGCTTTA GTGGGGCTTC GAAACTTAGT CATGCCTTTG
    AGAGCAAGCA ACTTCCATTA CCATGAGCTA AAGCCCATTG TGTTTGTGGG CTCGTTAGAT
    TATCTGCGAA GAGAGTGGGA AACCCTTCAC AACTTCCCCA AAGTCTTCAT CTTACCTGGT
    ACGCCATTAA GTCGAGCTGA TTTAAGGGCC GTCAACATCA ACCTCTGCGA CATGTGCGTT
    ATCCTGTCAG CCAATCAGAA CAATATCGAC GATGCTTCAC TGCAGGACAA GGAATGCATC
    TTGGCATCTC TTAACATCAA ATCTATGCAG TTTGATGACA GCATTGGGGT CTTGCAGGCT
    AATTCCCAAG GTTTCACACC TCCAGGGATG GATAGGTCAT CTCCTGATAA CAGTCCCGTG
    CACGGCTTAG TGCGGCAGGC ATCCGTCACC ACCGGATCAA ACATCCCCAT CATAACTGAA
    CTGGCTAAGC CTGGCAAACT ACTGCCTTTG GTTTCATTCA GTCAGGAAAA AAACAGTGGA
    ACCAACATAC AAATGATAAC AGAACTGGTG AACGACTCCA ACGTTCAGTT TTTGGACCAA
    GACGACGATG ACGACCCAGA CACTGAGCTC TACCTGACCC AGCCGTTCGC CTGTGGTACC
    GCATTCGCCG TCAGCGTGTT GGACTCCCTG ATGAGTGCGA CATACTTCAA TGATAATATC
    CTCACCCTGA TCCGGACGCT GGTCACGGGA GGCGCCACGC CGGAGCTGGA GGCCCTGTTG
    GCTGAGGAGA ACGCTCTGAG AGGTGGCTAC AGCACGCCGC AGACGCTGGC TAACAGGGAC
    AGGTGTCGGG TCGCCCAGCT GGCCCTCTAC GATGGGCCCT TCGCAGACTT GGGGGACGGA
    GGTTGCTATG GAGACTTGTT CTGTAAAGCA TTAAAAACCT ACAACATGCT GTGCTTTGGA
    ATCTATAGAT TGCGAGATGC ACATTTAGGT GCACCAAGCC AGTGCACAAA AAGATATGTC
    ATCACAAACC CGCCTTATGA GTTTGAGATG GTGCCCACGG ACCTGATCTT CTGTCTGATG
    CAGTTCGACC ACAACGCGGG CCAGTCCCGA GCGAGCCTGT CCCACTCCTC CCATTCGTCC
    CATTCCTCCA GCAAGAAGAG CTCGTCTGTT CACTCCATCC CAGCCACCAA CCGGCAGAAC
    CGCAGCAGCA AAGCACGTGA AGCCCGTGAC AAACAGAATG CAACAAGGAT GAATAGAATG
    GGCCCAGAAA AGAGATGGTT TACAGATGAA GCAGAAAACG CATACCCAAG GAACATTCAA
    ATCAAGCCTA TGAGCACTCA CATGGCCAAC CAAGTCAATC AGTACAAATC CACTAGCAGT
    CTGATTCCAC CTATCCGAGA AGTCGAAGAC GAATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18618
    Clone ID Related Accession (Same CDS sequence) XM_005156665.3 , XM_005156665.4
    Accession Version XM_005156665.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3690bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X14
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156665.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATCGAA GATGAGCATC CGTCAACACT GTCACCCAAA
    AAGAAGCAGC GCAACGGAGG CATGCGAAAT TCACCCAACT GCTCGCCCAA AATGATGAGG
    CATGATCCTC TACTCATTCC AGGAAATGAG CAGATTGAGA GCATGGATGC TAATGTCAAA
    CGATACGACT CTACTGGAAT GTTTCACTGG TGTCCATCCA AAGAAATCGA GAAGGTCATT
    CTGACACGCA GCGAGGCCTC AATGACGGTT TTAAGTGGAC ATGTCGTAGT CTGCATATTT
    GGAGATGTCA CGTCAGCTTT AGTGGGGCTT CGAAACTTAG TCATGCCTTT GAGAGCAAGC
    AACTTCCATT ACCATGAGCT AAAGCCCATT GTGTTTGTGG GCTCGTTAGA TTATCTGCGA
    AGAGAGTGGG AAACCCTTCA CAACTTCCCC AAAGTCTTCA TCTTACCTGG TACGCCATTA
    AGTCGAGCTG ATTTAAGGGC CGTCAACATC AACCTCTGCG ACATGTGCGT TATCCTGTCA
    GCCAATCAGA ACAATATCGA CGATGCTTCA CTGCAGGACA AGGAATGCAT CTTGGCATCT
    CTTAACATCA AATCTATGCA GTTTGATGAC AGCATTGGGG TCTTGCAGGC TAATTCCCAA
    GGTTTCACAC CTCCAGGGAT GGATAGGTCA TCTCCTGATA ACAGTCCCGT GCACGGCTTA
    GTGCGGCAGG CATCCGTCAC CACCGGATCA AACATCCCCA TCATAACTGA ACTGGCTAAG
    CCTGGCAAAC TACTGCCTTT GGTTTCATTC AGTCAGGAAA AAAACAGTGG AACCAACATA
    CAAATGATAA CAGAACTGGT GAACGACTCC AACGTTCAGT TTTTGGACCA AGACGACGAT
    GACGACCCAG ACACTGAGCT CTACCTGACC CAGCCGTTCG CCTGTGGTAC CGCATTCGCC
    GTCAGCGTGT TGGACTCCCT GATGAGTGCG ACATACTTCA ATGATAATAT CCTCACCCTG
    ATCCGGACGC TGGTCACGGG AGGCGCCACG CCGGAGCTGG AGGCCCTGTT GGCTGAGGAG
    AACGCTCTGA GAGGTGGCTA CAGCACGCCG CAGACGCTGG CTAACAGGGA CAGGTGTCGG
    GTCGCCCAGC TGGCCCTCTA CGATGGGCCC TTCGCAGACT TGGGGGACGG AGGTTGCTAT
    GGAGACTTGT TCTGTAAAGC ATTAAAAACC TACAACATGC TGTGCTTTGG AATCTATAGA
    TTGCGAGATG CACATTTAGG TGCACCAAGC CAGTGCACAA AAAGATATGT CATCACAAAC
    CCGCCTTATG AGTTTGAGAT GGTGCCCACG GACCTGATCT TCTGTCTGAT GCAGTTCGAC
    CACAACGCGG GCCAGTCCCG AGCGAGCCTG TCCCACTCCT CCCATTCGTC CCATTCCTCC
    AGCAAGAAGA GCTCGTCTGT TCACTCCATC CCAGCCACCA ACCGGCAGAA CCGCAGCAGC
    AAAGCACGTG AAGCCCGTGA CAAACAGAAT GCAACAAGGA TGAATAGAAT GGGCCCAGAA
    AAGAGATGGT TTACAGATGA AGCAGAAAAC GCATACCCAA GGAACATTCA AATCAAGCCT
    ATGAGCACTC ACATGGCCAA CCAAGTCAAT CAGTACAAAT CCACTAGCAG TCTGATTCCA
    CCTATCCGAG AAGTCGAAGA CGAATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156722.1
    CDS659..4348
    Translation

    Target ORF information:

    RefSeq Version XM_005156665.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156665.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATCGAA GATGAGCATC CGTCAACACT GTCACCCAAA
    AAGAAGCAGC GCAACGGAGG CATGCGAAAT TCACCCAACT GCTCGCCCAA AATGATGAGG
    CATGATCCTC TACTCATTCC AGGAAATGAG CAGATTGAGA GCATGGATGC TAATGTCAAA
    CGATACGACT CTACTGGAAT GTTTCACTGG TGTCCATCCA AAGAAATCGA GAAGGTCATT
    CTGACACGCA GCGAGGCCTC AATGACGGTT TTAAGTGGAC ATGTCGTAGT CTGCATATTT
    GGAGATGTCA CGTCAGCTTT AGTGGGGCTT CGAAACTTAG TCATGCCTTT GAGAGCAAGC
    AACTTCCATT ACCATGAGCT AAAGCCCATT GTGTTTGTGG GCTCGTTAGA TTATCTGCGA
    AGAGAGTGGG AAACCCTTCA CAACTTCCCC AAAGTCTTCA TCTTACCTGG TACGCCATTA
    AGTCGAGCTG ATTTAAGGGC CGTCAACATC AACCTCTGCG ACATGTGCGT TATCCTGTCA
    GCCAATCAGA ACAATATCGA CGATGCTTCA CTGCAGGACA AGGAATGCAT CTTGGCATCT
    CTTAACATCA AATCTATGCA GTTTGATGAC AGCATTGGGG TCTTGCAGGC TAATTCCCAA
    GGTTTCACAC CTCCAGGGAT GGATAGGTCA TCTCCTGATA ACAGTCCCGT GCACGGCTTA
    GTGCGGCAGG CATCCGTCAC CACCGGATCA AACATCCCCA TCATAACTGA ACTGGCTAAG
    CCTGGCAAAC TACTGCCTTT GGTTTCATTC AGTCAGGAAA AAAACAGTGG AACCAACATA
    CAAATGATAA CAGAACTGGT GAACGACTCC AACGTTCAGT TTTTGGACCA AGACGACGAT
    GACGACCCAG ACACTGAGCT CTACCTGACC CAGCCGTTCG CCTGTGGTAC CGCATTCGCC
    GTCAGCGTGT TGGACTCCCT GATGAGTGCG ACATACTTCA ATGATAATAT CCTCACCCTG
    ATCCGGACGC TGGTCACGGG AGGCGCCACG CCGGAGCTGG AGGCCCTGTT GGCTGAGGAG
    AACGCTCTGA GAGGTGGCTA CAGCACGCCG CAGACGCTGG CTAACAGGGA CAGGTGTCGG
    GTCGCCCAGC TGGCCCTCTA CGATGGGCCC TTCGCAGACT TGGGGGACGG AGGTTGCTAT
    GGAGACTTGT TCTGTAAAGC ATTAAAAACC TACAACATGC TGTGCTTTGG AATCTATAGA
    TTGCGAGATG CACATTTAGG TGCACCAAGC CAGTGCACAA AAAGATATGT CATCACAAAC
    CCGCCTTATG AGTTTGAGAT GGTGCCCACG GACCTGATCT TCTGTCTGAT GCAGTTCGAC
    CACAACGCGG GCCAGTCCCG AGCGAGCCTG TCCCACTCCT CCCATTCGTC CCATTCCTCC
    AGCAAGAAGA GCTCGTCTGT TCACTCCATC CCAGCCACCA ACCGGCAGAA CCGCAGCAGC
    AAAGCACGTG AAGCCCGTGA CAAACAGAAT GCAACAAGGA TGAATAGAAT GGGCCCAGAA
    AAGAGATGGT TTACAGATGA AGCAGAAAAC GCATACCCAA GGAACATTCA AATCAAGCCT
    ATGAGCACTC ACATGGCCAA CCAAGTCAAT CAGTACAAAT CCACTAGCAG TCTGATTCCA
    CCTATCCGAG AAGTCGAAGA CGAATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18618
    Clone ID Related Accession (Same CDS sequence) XM_005156665.3 , XM_005156665.4
    Accession Version XM_005156665.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3690bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X16
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156665.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATCGAA GATGAGCATC CGTCAACACT GTCACCCAAA
    AAGAAGCAGC GCAACGGAGG CATGCGAAAT TCACCCAACT GCTCGCCCAA AATGATGAGG
    CATGATCCTC TACTCATTCC AGGAAATGAG CAGATTGAGA GCATGGATGC TAATGTCAAA
    CGATACGACT CTACTGGAAT GTTTCACTGG TGTCCATCCA AAGAAATCGA GAAGGTCATT
    CTGACACGCA GCGAGGCCTC AATGACGGTT TTAAGTGGAC ATGTCGTAGT CTGCATATTT
    GGAGATGTCA CGTCAGCTTT AGTGGGGCTT CGAAACTTAG TCATGCCTTT GAGAGCAAGC
    AACTTCCATT ACCATGAGCT AAAGCCCATT GTGTTTGTGG GCTCGTTAGA TTATCTGCGA
    AGAGAGTGGG AAACCCTTCA CAACTTCCCC AAAGTCTTCA TCTTACCTGG TACGCCATTA
    AGTCGAGCTG ATTTAAGGGC CGTCAACATC AACCTCTGCG ACATGTGCGT TATCCTGTCA
    GCCAATCAGA ACAATATCGA CGATGCTTCA CTGCAGGACA AGGAATGCAT CTTGGCATCT
    CTTAACATCA AATCTATGCA GTTTGATGAC AGCATTGGGG TCTTGCAGGC TAATTCCCAA
    GGTTTCACAC CTCCAGGGAT GGATAGGTCA TCTCCTGATA ACAGTCCCGT GCACGGCTTA
    GTGCGGCAGG CATCCGTCAC CACCGGATCA AACATCCCCA TCATAACTGA ACTGGCTAAG
    CCTGGCAAAC TACTGCCTTT GGTTTCATTC AGTCAGGAAA AAAACAGTGG AACCAACATA
    CAAATGATAA CAGAACTGGT GAACGACTCC AACGTTCAGT TTTTGGACCA AGACGACGAT
    GACGACCCAG ACACTGAGCT CTACCTGACC CAGCCGTTCG CCTGTGGTAC CGCATTCGCC
    GTCAGCGTGT TGGACTCCCT GATGAGTGCG ACATACTTCA ATGATAATAT CCTCACCCTG
    ATCCGGACGC TGGTCACGGG AGGCGCCACG CCGGAGCTGG AGGCCCTGTT GGCTGAGGAG
    AACGCTCTGA GAGGTGGCTA CAGCACGCCG CAGACGCTGG CTAACAGGGA CAGGTGTCGG
    GTCGCCCAGC TGGCCCTCTA CGATGGGCCC TTCGCAGACT TGGGGGACGG AGGTTGCTAT
    GGAGACTTGT TCTGTAAAGC ATTAAAAACC TACAACATGC TGTGCTTTGG AATCTATAGA
    TTGCGAGATG CACATTTAGG TGCACCAAGC CAGTGCACAA AAAGATATGT CATCACAAAC
    CCGCCTTATG AGTTTGAGAT GGTGCCCACG GACCTGATCT TCTGTCTGAT GCAGTTCGAC
    CACAACGCGG GCCAGTCCCG AGCGAGCCTG TCCCACTCCT CCCATTCGTC CCATTCCTCC
    AGCAAGAAGA GCTCGTCTGT TCACTCCATC CCAGCCACCA ACCGGCAGAA CCGCAGCAGC
    AAAGCACGTG AAGCCCGTGA CAAACAGAAT GCAACAAGGA TGAATAGAAT GGGCCCAGAA
    AAGAGATGGT TTACAGATGA AGCAGAAAAC GCATACCCAA GGAACATTCA AATCAAGCCT
    ATGAGCACTC ACATGGCCAA CCAAGTCAAT CAGTACAAAT CCACTAGCAG TCTGATTCCA
    CCTATCCGAG AAGTCGAAGA CGAATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156722.1
    CDS676..4365
    Translation

    Target ORF information:

    RefSeq Version XM_005156665.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156665.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATCGAA GATGAGCATC CGTCAACACT GTCACCCAAA
    AAGAAGCAGC GCAACGGAGG CATGCGAAAT TCACCCAACT GCTCGCCCAA AATGATGAGG
    CATGATCCTC TACTCATTCC AGGAAATGAG CAGATTGAGA GCATGGATGC TAATGTCAAA
    CGATACGACT CTACTGGAAT GTTTCACTGG TGTCCATCCA AAGAAATCGA GAAGGTCATT
    CTGACACGCA GCGAGGCCTC AATGACGGTT TTAAGTGGAC ATGTCGTAGT CTGCATATTT
    GGAGATGTCA CGTCAGCTTT AGTGGGGCTT CGAAACTTAG TCATGCCTTT GAGAGCAAGC
    AACTTCCATT ACCATGAGCT AAAGCCCATT GTGTTTGTGG GCTCGTTAGA TTATCTGCGA
    AGAGAGTGGG AAACCCTTCA CAACTTCCCC AAAGTCTTCA TCTTACCTGG TACGCCATTA
    AGTCGAGCTG ATTTAAGGGC CGTCAACATC AACCTCTGCG ACATGTGCGT TATCCTGTCA
    GCCAATCAGA ACAATATCGA CGATGCTTCA CTGCAGGACA AGGAATGCAT CTTGGCATCT
    CTTAACATCA AATCTATGCA GTTTGATGAC AGCATTGGGG TCTTGCAGGC TAATTCCCAA
    GGTTTCACAC CTCCAGGGAT GGATAGGTCA TCTCCTGATA ACAGTCCCGT GCACGGCTTA
    GTGCGGCAGG CATCCGTCAC CACCGGATCA AACATCCCCA TCATAACTGA ACTGGCTAAG
    CCTGGCAAAC TACTGCCTTT GGTTTCATTC AGTCAGGAAA AAAACAGTGG AACCAACATA
    CAAATGATAA CAGAACTGGT GAACGACTCC AACGTTCAGT TTTTGGACCA AGACGACGAT
    GACGACCCAG ACACTGAGCT CTACCTGACC CAGCCGTTCG CCTGTGGTAC CGCATTCGCC
    GTCAGCGTGT TGGACTCCCT GATGAGTGCG ACATACTTCA ATGATAATAT CCTCACCCTG
    ATCCGGACGC TGGTCACGGG AGGCGCCACG CCGGAGCTGG AGGCCCTGTT GGCTGAGGAG
    AACGCTCTGA GAGGTGGCTA CAGCACGCCG CAGACGCTGG CTAACAGGGA CAGGTGTCGG
    GTCGCCCAGC TGGCCCTCTA CGATGGGCCC TTCGCAGACT TGGGGGACGG AGGTTGCTAT
    GGAGACTTGT TCTGTAAAGC ATTAAAAACC TACAACATGC TGTGCTTTGG AATCTATAGA
    TTGCGAGATG CACATTTAGG TGCACCAAGC CAGTGCACAA AAAGATATGT CATCACAAAC
    CCGCCTTATG AGTTTGAGAT GGTGCCCACG GACCTGATCT TCTGTCTGAT GCAGTTCGAC
    CACAACGCGG GCCAGTCCCG AGCGAGCCTG TCCCACTCCT CCCATTCGTC CCATTCCTCC
    AGCAAGAAGA GCTCGTCTGT TCACTCCATC CCAGCCACCA ACCGGCAGAA CCGCAGCAGC
    AAAGCACGTG AAGCCCGTGA CAAACAGAAT GCAACAAGGA TGAATAGAAT GGGCCCAGAA
    AAGAGATGGT TTACAGATGA AGCAGAAAAC GCATACCCAA GGAACATTCA AATCAAGCCT
    ATGAGCACTC ACATGGCCAA CCAAGTCAAT CAGTACAAAT CCACTAGCAG TCTGATTCCA
    CCTATCCGAG AAGTCGAAGA CGAATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18620
    Clone ID Related Accession (Same CDS sequence) XM_005156667.4 , XM_005156667.3
    Accession Version XM_005156667.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X19
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156667.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAACCA AGATGTCCGT CTACAAGCGA
    ATGAAACTGG CATGTTGTTT TGATTGCGGG CGCTCTGAGC GCGACTGCTC GTGCATGTCA
    GGCAGTGTAC ATAGTAACAT GGACAGCCTT CAGAGGGCCT TCCCACTTTC TTCTGTCTCT
    GTTCATGATT GCTCAACCAG TTTACGTGCC TTCGAAGATG AGCATCCGTC AACACTGTCA
    CCCAAAAAGA AGCAGCGCAA CGGAGGCATG CGAAATTCAC CCAACTGCTC GCCCAAAATG
    ATGAGGCATG ATCCTCTACT CATTCCAGGA AATGAGCAGA TTGAGAGCAT GGATGCTAAT
    GTCAAACGAT ACGACTCTAC TGGAATGTTT CACTGGTGTC CATCCAAAGA AATCGAGAAG
    GTCATTCTGA CACGCAGCGA GGCCTCAATG ACGGTTTTAA GTGGACATGT CGTAGTCTGC
    ATATTTGGAG ATGTCACGTC AGCTTTAGTG GGGCTTCGAA ACTTAGTCAT GCCTTTGAGA
    GCAAGCAACT TCCATTACCA TGAGCTAAAG CCCATTGTGT TTGTGGGCTC GTTAGATTAT
    CTGCGAAGAG AGTGGGAAAC CCTTCACAAC TTCCCCAAAG TCTTCATCTT ACCTGGTACG
    CCATTAAGTC GAGCTGATTT AAGGGCCGTC AACATCAACC TCTGCGACAT GTGCGTTATC
    CTGTCAGCCA ATCAGAACAA TATCGACGAT GCTTCACTGC AGGACAAGGA ATGCATCTTG
    GCATCTCTTA ACATCAAATC TATGCAGTTT GATGACAGCA TTGGGGTCTT GCAGGCTAAT
    TCCCAAGGTT TCACACCTCC AGGGATGGAT AGGTCATCTC CTGATAACAG TCCCGTGCAC
    GGCTTAGTGC GGCAGGCATC CGTCACCACC GGATCAAACA TCCCCATCAT AACTGAACTG
    GTGAACGACT CCAACGTTCA GTTTTTGGAC CAAGACGACG ATGACGACCC AGACACTGAG
    CTCTACCTGA CCCAGCCGTT CGCCTGTGGT ACCGCATTCG CCGTCAGCGT GTTGGACTCC
    CTGATGAGTG CGACATACTT CAATGATAAT ATCCTCACCC TGATCCGGAC GCTGGTCACG
    GGAGGCGCCA CGCCGGAGCT GGAGGCCCTG TTGGCTGAGG AGAACGCTCT GAGAGGTGGC
    TACAGCACGC CGCAGACGCT GGCTAACAGG GACAGGTGTC GGGTCGCCCA GCTGGCCCTC
    TACGATGGGC CCTTCGCAGA CTTGGGGGAC GGAGGTTGCT ATGGAGACTT GTTCTGTAAA
    GCATTAAAAA CCTACAACAT GCTGTGCTTT GGAATCTATA GATTGCGAGA TGCACATTTA
    GGTGCACCAA GCCAGTGCAC AAAAAGATAT GTCATCACAA ACCCGCCTTA TGAGTTTGAG
    ATGGTGCCCA CGGACCTGAT CTTCTGTCTG ATGCAGTTCG ACCACAACGC GGGCCAGTCC
    CGAGCGAGCC TGTCCCACTC CTCCCATTCG TCCCATTCCT CCAGCAAGAA GAGCTCGTCT
    GTTCACTCCA TCCCAGCCAC CAACCGGCAG AACCGCAGCA GCAAAGCACG TGAAGCCCGT
    GACAAACAGA AAAAAGAGAT GGTTTACAGA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156724.1
    CDS676..4308
    Translation

    Target ORF information:

    RefSeq Version XM_005156667.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156667.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAACCA AGATGTCCGT CTACAAGCGA
    ATGAAACTGG CATGTTGTTT TGATTGCGGG CGCTCTGAGC GCGACTGCTC GTGCATGTCA
    GGCAGTGTAC ATAGTAACAT GGACAGCCTT CAGAGGGCCT TCCCACTTTC TTCTGTCTCT
    GTTCATGATT GCTCAACCAG TTTACGTGCC TTCGAAGATG AGCATCCGTC AACACTGTCA
    CCCAAAAAGA AGCAGCGCAA CGGAGGCATG CGAAATTCAC CCAACTGCTC GCCCAAAATG
    ATGAGGCATG ATCCTCTACT CATTCCAGGA AATGAGCAGA TTGAGAGCAT GGATGCTAAT
    GTCAAACGAT ACGACTCTAC TGGAATGTTT CACTGGTGTC CATCCAAAGA AATCGAGAAG
    GTCATTCTGA CACGCAGCGA GGCCTCAATG ACGGTTTTAA GTGGACATGT CGTAGTCTGC
    ATATTTGGAG ATGTCACGTC AGCTTTAGTG GGGCTTCGAA ACTTAGTCAT GCCTTTGAGA
    GCAAGCAACT TCCATTACCA TGAGCTAAAG CCCATTGTGT TTGTGGGCTC GTTAGATTAT
    CTGCGAAGAG AGTGGGAAAC CCTTCACAAC TTCCCCAAAG TCTTCATCTT ACCTGGTACG
    CCATTAAGTC GAGCTGATTT AAGGGCCGTC AACATCAACC TCTGCGACAT GTGCGTTATC
    CTGTCAGCCA ATCAGAACAA TATCGACGAT GCTTCACTGC AGGACAAGGA ATGCATCTTG
    GCATCTCTTA ACATCAAATC TATGCAGTTT GATGACAGCA TTGGGGTCTT GCAGGCTAAT
    TCCCAAGGTT TCACACCTCC AGGGATGGAT AGGTCATCTC CTGATAACAG TCCCGTGCAC
    GGCTTAGTGC GGCAGGCATC CGTCACCACC GGATCAAACA TCCCCATCAT AACTGAACTG
    GTGAACGACT CCAACGTTCA GTTTTTGGAC CAAGACGACG ATGACGACCC AGACACTGAG
    CTCTACCTGA CCCAGCCGTT CGCCTGTGGT ACCGCATTCG CCGTCAGCGT GTTGGACTCC
    CTGATGAGTG CGACATACTT CAATGATAAT ATCCTCACCC TGATCCGGAC GCTGGTCACG
    GGAGGCGCCA CGCCGGAGCT GGAGGCCCTG TTGGCTGAGG AGAACGCTCT GAGAGGTGGC
    TACAGCACGC CGCAGACGCT GGCTAACAGG GACAGGTGTC GGGTCGCCCA GCTGGCCCTC
    TACGATGGGC CCTTCGCAGA CTTGGGGGAC GGAGGTTGCT ATGGAGACTT GTTCTGTAAA
    GCATTAAAAA CCTACAACAT GCTGTGCTTT GGAATCTATA GATTGCGAGA TGCACATTTA
    GGTGCACCAA GCCAGTGCAC AAAAAGATAT GTCATCACAA ACCCGCCTTA TGAGTTTGAG
    ATGGTGCCCA CGGACCTGAT CTTCTGTCTG ATGCAGTTCG ACCACAACGC GGGCCAGTCC
    CGAGCGAGCC TGTCCCACTC CTCCCATTCG TCCCATTCCT CCAGCAAGAA GAGCTCGTCT
    GTTCACTCCA TCCCAGCCAC CAACCGGCAG AACCGCAGCA GCAAAGCACG TGAAGCCCGT
    GACAAACAGA AAAAAGAGAT GGTTTACAGA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18620
    Clone ID Related Accession (Same CDS sequence) XM_005156667.4 , XM_005156667.3
    Accession Version XM_005156667.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3633bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X17
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156667.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAACCA AGATGTCCGT CTACAAGCGA
    ATGAAACTGG CATGTTGTTT TGATTGCGGG CGCTCTGAGC GCGACTGCTC GTGCATGTCA
    GGCAGTGTAC ATAGTAACAT GGACAGCCTT CAGAGGGCCT TCCCACTTTC TTCTGTCTCT
    GTTCATGATT GCTCAACCAG TTTACGTGCC TTCGAAGATG AGCATCCGTC AACACTGTCA
    CCCAAAAAGA AGCAGCGCAA CGGAGGCATG CGAAATTCAC CCAACTGCTC GCCCAAAATG
    ATGAGGCATG ATCCTCTACT CATTCCAGGA AATGAGCAGA TTGAGAGCAT GGATGCTAAT
    GTCAAACGAT ACGACTCTAC TGGAATGTTT CACTGGTGTC CATCCAAAGA AATCGAGAAG
    GTCATTCTGA CACGCAGCGA GGCCTCAATG ACGGTTTTAA GTGGACATGT CGTAGTCTGC
    ATATTTGGAG ATGTCACGTC AGCTTTAGTG GGGCTTCGAA ACTTAGTCAT GCCTTTGAGA
    GCAAGCAACT TCCATTACCA TGAGCTAAAG CCCATTGTGT TTGTGGGCTC GTTAGATTAT
    CTGCGAAGAG AGTGGGAAAC CCTTCACAAC TTCCCCAAAG TCTTCATCTT ACCTGGTACG
    CCATTAAGTC GAGCTGATTT AAGGGCCGTC AACATCAACC TCTGCGACAT GTGCGTTATC
    CTGTCAGCCA ATCAGAACAA TATCGACGAT GCTTCACTGC AGGACAAGGA ATGCATCTTG
    GCATCTCTTA ACATCAAATC TATGCAGTTT GATGACAGCA TTGGGGTCTT GCAGGCTAAT
    TCCCAAGGTT TCACACCTCC AGGGATGGAT AGGTCATCTC CTGATAACAG TCCCGTGCAC
    GGCTTAGTGC GGCAGGCATC CGTCACCACC GGATCAAACA TCCCCATCAT AACTGAACTG
    GTGAACGACT CCAACGTTCA GTTTTTGGAC CAAGACGACG ATGACGACCC AGACACTGAG
    CTCTACCTGA CCCAGCCGTT CGCCTGTGGT ACCGCATTCG CCGTCAGCGT GTTGGACTCC
    CTGATGAGTG CGACATACTT CAATGATAAT ATCCTCACCC TGATCCGGAC GCTGGTCACG
    GGAGGCGCCA CGCCGGAGCT GGAGGCCCTG TTGGCTGAGG AGAACGCTCT GAGAGGTGGC
    TACAGCACGC CGCAGACGCT GGCTAACAGG GACAGGTGTC GGGTCGCCCA GCTGGCCCTC
    TACGATGGGC CCTTCGCAGA CTTGGGGGAC GGAGGTTGCT ATGGAGACTT GTTCTGTAAA
    GCATTAAAAA CCTACAACAT GCTGTGCTTT GGAATCTATA GATTGCGAGA TGCACATTTA
    GGTGCACCAA GCCAGTGCAC AAAAAGATAT GTCATCACAA ACCCGCCTTA TGAGTTTGAG
    ATGGTGCCCA CGGACCTGAT CTTCTGTCTG ATGCAGTTCG ACCACAACGC GGGCCAGTCC
    CGAGCGAGCC TGTCCCACTC CTCCCATTCG TCCCATTCCT CCAGCAAGAA GAGCTCGTCT
    GTTCACTCCA TCCCAGCCAC CAACCGGCAG AACCGCAGCA GCAAAGCACG TGAAGCCCGT
    GACAAACAGA AAAAAGAGAT GGTTTACAGA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156724.1
    CDS656..4288
    Translation

    Target ORF information:

    RefSeq Version XM_005156667.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X17, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156667.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAACCA AGATGTCCGT CTACAAGCGA
    ATGAAACTGG CATGTTGTTT TGATTGCGGG CGCTCTGAGC GCGACTGCTC GTGCATGTCA
    GGCAGTGTAC ATAGTAACAT GGACAGCCTT CAGAGGGCCT TCCCACTTTC TTCTGTCTCT
    GTTCATGATT GCTCAACCAG TTTACGTGCC TTCGAAGATG AGCATCCGTC AACACTGTCA
    CCCAAAAAGA AGCAGCGCAA CGGAGGCATG CGAAATTCAC CCAACTGCTC GCCCAAAATG
    ATGAGGCATG ATCCTCTACT CATTCCAGGA AATGAGCAGA TTGAGAGCAT GGATGCTAAT
    GTCAAACGAT ACGACTCTAC TGGAATGTTT CACTGGTGTC CATCCAAAGA AATCGAGAAG
    GTCATTCTGA CACGCAGCGA GGCCTCAATG ACGGTTTTAA GTGGACATGT CGTAGTCTGC
    ATATTTGGAG ATGTCACGTC AGCTTTAGTG GGGCTTCGAA ACTTAGTCAT GCCTTTGAGA
    GCAAGCAACT TCCATTACCA TGAGCTAAAG CCCATTGTGT TTGTGGGCTC GTTAGATTAT
    CTGCGAAGAG AGTGGGAAAC CCTTCACAAC TTCCCCAAAG TCTTCATCTT ACCTGGTACG
    CCATTAAGTC GAGCTGATTT AAGGGCCGTC AACATCAACC TCTGCGACAT GTGCGTTATC
    CTGTCAGCCA ATCAGAACAA TATCGACGAT GCTTCACTGC AGGACAAGGA ATGCATCTTG
    GCATCTCTTA ACATCAAATC TATGCAGTTT GATGACAGCA TTGGGGTCTT GCAGGCTAAT
    TCCCAAGGTT TCACACCTCC AGGGATGGAT AGGTCATCTC CTGATAACAG TCCCGTGCAC
    GGCTTAGTGC GGCAGGCATC CGTCACCACC GGATCAAACA TCCCCATCAT AACTGAACTG
    GTGAACGACT CCAACGTTCA GTTTTTGGAC CAAGACGACG ATGACGACCC AGACACTGAG
    CTCTACCTGA CCCAGCCGTT CGCCTGTGGT ACCGCATTCG CCGTCAGCGT GTTGGACTCC
    CTGATGAGTG CGACATACTT CAATGATAAT ATCCTCACCC TGATCCGGAC GCTGGTCACG
    GGAGGCGCCA CGCCGGAGCT GGAGGCCCTG TTGGCTGAGG AGAACGCTCT GAGAGGTGGC
    TACAGCACGC CGCAGACGCT GGCTAACAGG GACAGGTGTC GGGTCGCCCA GCTGGCCCTC
    TACGATGGGC CCTTCGCAGA CTTGGGGGAC GGAGGTTGCT ATGGAGACTT GTTCTGTAAA
    GCATTAAAAA CCTACAACAT GCTGTGCTTT GGAATCTATA GATTGCGAGA TGCACATTTA
    GGTGCACCAA GCCAGTGCAC AAAAAGATAT GTCATCACAA ACCCGCCTTA TGAGTTTGAG
    ATGGTGCCCA CGGACCTGAT CTTCTGTCTG ATGCAGTTCG ACCACAACGC GGGCCAGTCC
    CGAGCGAGCC TGTCCCACTC CTCCCATTCG TCCCATTCCT CCAGCAAGAA GAGCTCGTCT
    GTTCACTCCA TCCCAGCCAC CAACCGGCAG AACCGCAGCA GCAAAGCACG TGAAGCCCGT
    GACAAACAGA AAAAAGAGAT GGTTTACAGA TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18621
    Clone ID Related Accession (Same CDS sequence) XM_005156668.3 , XM_005156668.4
    Accession Version XM_005156668.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3627bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X18
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156668.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTTCGA AGATGAGCAT
    CCGTCAACAC TGTCACCCAA AAAGAAGCAG CGCAACGGAG GCATGCGAAA TTCACCCAAC
    TGCTCGCCCA AAATGATGAG GCATGATCCT CTACTCATTC CAGGAAATGA GCAGATTGAG
    AGCATGGATG CTAATGTCAA ACGATACGAC TCTACTGGAA TGTTTCACTG GTGTCCATCC
    AAAGAAATCG AGAAGGTCAT TCTGACACGC AGCGAGGCCT CAATGACGGT TTTAAGTGGA
    CATGTCGTAG TCTGCATATT TGGAGATGTC ACGTCAGCTT TAGTGGGGCT TCGAAACTTA
    GTCATGCCTT TGAGAGCAAG CAACTTCCAT TACCATGAGC TAAAGCCCAT TGTGTTTGTG
    GGCTCGTTAG ATTATCTGCG AAGAGAGTGG GAAACCCTTC ACAACTTCCC CAAAGTCTTC
    ATCTTACCTG GTACGCCATT AAGTCGAGCT GATTTAAGGG CCGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AACAATATCG ACGATGCTTC ACTGCAGGAC
    AAGGAATGCA TCTTGGCATC TCTTAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGTTTCACA CCTCCAGGGA TGGATAGGTC ATCTCCTGAT
    AACAGTCCCG TGCACGGCTT AGTGCGGCAG GCATCCGTCA CCACCGGATC AAACATCCCC
    ATCATAACTG AACTGGTGAA CGACTCCAAC GTTCAGTTTT TGGACCAAGA CGACGATGAC
    GACCCAGACA CTGAGCTCTA CCTGACCCAG CCGTTCGCCT GTGGTACCGC ATTCGCCGTC
    AGCGTGTTGG ACTCCCTGAT GAGTGCGACA TACTTCAATG ATAATATCCT CACCCTGATC
    CGGACGCTGG TCACGGGAGG CGCCACGCCG GAGCTGGAGG CCCTGTTGGC TGAGGAGAAC
    GCTCTGAGAG GTGGCTACAG CACGCCGCAG ACGCTGGCTA ACAGGGACAG GTGTCGGGTC
    GCCCAGCTGG CCCTCTACGA TGGGCCCTTC GCAGACTTGG GGGACGGAGG TTGCTATGGA
    GACTTGTTCT GTAAAGCATT AAAAACCTAC AACATGCTGT GCTTTGGAAT CTATAGATTG
    CGAGATGCAC ATTTAGGTGC ACCAAGCCAG TGCACAAAAA GATATGTCAT CACAAACCCG
    CCTTATGAGT TTGAGATGGT GCCCACGGAC CTGATCTTCT GTCTGATGCA GTTCGACCAC
    AACGCGGGCC AGTCCCGAGC GAGCCTGTCC CACTCCTCCC ATTCGTCCCA TTCCTCCAGC
    AAGAAGAGCT CGTCTGTTCA CTCCATCCCA GCCACCAACC GGCAGAACCG CAGCAGCAAA
    GCACGTGAAG CCCGTGACAA ACAGAATGCA ACAAGGATGA ATAGAATGGG CCCAGAAAAG
    AGATGGTTTA CAGATGAAGC AGAAAACGCA TACCCAAGGA ACATTCAAAT CAAGCCTATG
    AGCACTCACA TGGCCAACCA AGTCAATCAG TACAAATCCA CTAGCAGTCT GATTCCACCT
    ATCCGAGAAG TCGAAGACGA ATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156725.1
    CDS658..4284
    Translation

    Target ORF information:

    RefSeq Version XM_005156668.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156668.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTTCGA AGATGAGCAT
    CCGTCAACAC TGTCACCCAA AAAGAAGCAG CGCAACGGAG GCATGCGAAA TTCACCCAAC
    TGCTCGCCCA AAATGATGAG GCATGATCCT CTACTCATTC CAGGAAATGA GCAGATTGAG
    AGCATGGATG CTAATGTCAA ACGATACGAC TCTACTGGAA TGTTTCACTG GTGTCCATCC
    AAAGAAATCG AGAAGGTCAT TCTGACACGC AGCGAGGCCT CAATGACGGT TTTAAGTGGA
    CATGTCGTAG TCTGCATATT TGGAGATGTC ACGTCAGCTT TAGTGGGGCT TCGAAACTTA
    GTCATGCCTT TGAGAGCAAG CAACTTCCAT TACCATGAGC TAAAGCCCAT TGTGTTTGTG
    GGCTCGTTAG ATTATCTGCG AAGAGAGTGG GAAACCCTTC ACAACTTCCC CAAAGTCTTC
    ATCTTACCTG GTACGCCATT AAGTCGAGCT GATTTAAGGG CCGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AACAATATCG ACGATGCTTC ACTGCAGGAC
    AAGGAATGCA TCTTGGCATC TCTTAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGTTTCACA CCTCCAGGGA TGGATAGGTC ATCTCCTGAT
    AACAGTCCCG TGCACGGCTT AGTGCGGCAG GCATCCGTCA CCACCGGATC AAACATCCCC
    ATCATAACTG AACTGGTGAA CGACTCCAAC GTTCAGTTTT TGGACCAAGA CGACGATGAC
    GACCCAGACA CTGAGCTCTA CCTGACCCAG CCGTTCGCCT GTGGTACCGC ATTCGCCGTC
    AGCGTGTTGG ACTCCCTGAT GAGTGCGACA TACTTCAATG ATAATATCCT CACCCTGATC
    CGGACGCTGG TCACGGGAGG CGCCACGCCG GAGCTGGAGG CCCTGTTGGC TGAGGAGAAC
    GCTCTGAGAG GTGGCTACAG CACGCCGCAG ACGCTGGCTA ACAGGGACAG GTGTCGGGTC
    GCCCAGCTGG CCCTCTACGA TGGGCCCTTC GCAGACTTGG GGGACGGAGG TTGCTATGGA
    GACTTGTTCT GTAAAGCATT AAAAACCTAC AACATGCTGT GCTTTGGAAT CTATAGATTG
    CGAGATGCAC ATTTAGGTGC ACCAAGCCAG TGCACAAAAA GATATGTCAT CACAAACCCG
    CCTTATGAGT TTGAGATGGT GCCCACGGAC CTGATCTTCT GTCTGATGCA GTTCGACCAC
    AACGCGGGCC AGTCCCGAGC GAGCCTGTCC CACTCCTCCC ATTCGTCCCA TTCCTCCAGC
    AAGAAGAGCT CGTCTGTTCA CTCCATCCCA GCCACCAACC GGCAGAACCG CAGCAGCAAA
    GCACGTGAAG CCCGTGACAA ACAGAATGCA ACAAGGATGA ATAGAATGGG CCCAGAAAAG
    AGATGGTTTA CAGATGAAGC AGAAAACGCA TACCCAAGGA ACATTCAAAT CAAGCCTATG
    AGCACTCACA TGGCCAACCA AGTCAATCAG TACAAATCCA CTAGCAGTCT GATTCCACCT
    ATCCGAGAAG TCGAAGACGA ATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18621
    Clone ID Related Accession (Same CDS sequence) XM_005156668.3 , XM_005156668.4
    Accession Version XM_005156668.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3627bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X20
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156668.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTTCGA AGATGAGCAT
    CCGTCAACAC TGTCACCCAA AAAGAAGCAG CGCAACGGAG GCATGCGAAA TTCACCCAAC
    TGCTCGCCCA AAATGATGAG GCATGATCCT CTACTCATTC CAGGAAATGA GCAGATTGAG
    AGCATGGATG CTAATGTCAA ACGATACGAC TCTACTGGAA TGTTTCACTG GTGTCCATCC
    AAAGAAATCG AGAAGGTCAT TCTGACACGC AGCGAGGCCT CAATGACGGT TTTAAGTGGA
    CATGTCGTAG TCTGCATATT TGGAGATGTC ACGTCAGCTT TAGTGGGGCT TCGAAACTTA
    GTCATGCCTT TGAGAGCAAG CAACTTCCAT TACCATGAGC TAAAGCCCAT TGTGTTTGTG
    GGCTCGTTAG ATTATCTGCG AAGAGAGTGG GAAACCCTTC ACAACTTCCC CAAAGTCTTC
    ATCTTACCTG GTACGCCATT AAGTCGAGCT GATTTAAGGG CCGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AACAATATCG ACGATGCTTC ACTGCAGGAC
    AAGGAATGCA TCTTGGCATC TCTTAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGTTTCACA CCTCCAGGGA TGGATAGGTC ATCTCCTGAT
    AACAGTCCCG TGCACGGCTT AGTGCGGCAG GCATCCGTCA CCACCGGATC AAACATCCCC
    ATCATAACTG AACTGGTGAA CGACTCCAAC GTTCAGTTTT TGGACCAAGA CGACGATGAC
    GACCCAGACA CTGAGCTCTA CCTGACCCAG CCGTTCGCCT GTGGTACCGC ATTCGCCGTC
    AGCGTGTTGG ACTCCCTGAT GAGTGCGACA TACTTCAATG ATAATATCCT CACCCTGATC
    CGGACGCTGG TCACGGGAGG CGCCACGCCG GAGCTGGAGG CCCTGTTGGC TGAGGAGAAC
    GCTCTGAGAG GTGGCTACAG CACGCCGCAG ACGCTGGCTA ACAGGGACAG GTGTCGGGTC
    GCCCAGCTGG CCCTCTACGA TGGGCCCTTC GCAGACTTGG GGGACGGAGG TTGCTATGGA
    GACTTGTTCT GTAAAGCATT AAAAACCTAC AACATGCTGT GCTTTGGAAT CTATAGATTG
    CGAGATGCAC ATTTAGGTGC ACCAAGCCAG TGCACAAAAA GATATGTCAT CACAAACCCG
    CCTTATGAGT TTGAGATGGT GCCCACGGAC CTGATCTTCT GTCTGATGCA GTTCGACCAC
    AACGCGGGCC AGTCCCGAGC GAGCCTGTCC CACTCCTCCC ATTCGTCCCA TTCCTCCAGC
    AAGAAGAGCT CGTCTGTTCA CTCCATCCCA GCCACCAACC GGCAGAACCG CAGCAGCAAA
    GCACGTGAAG CCCGTGACAA ACAGAATGCA ACAAGGATGA ATAGAATGGG CCCAGAAAAG
    AGATGGTTTA CAGATGAAGC AGAAAACGCA TACCCAAGGA ACATTCAAAT CAAGCCTATG
    AGCACTCACA TGGCCAACCA AGTCAATCAG TACAAATCCA CTAGCAGTCT GATTCCACCT
    ATCCGAGAAG TCGAAGACGA ATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156725.1
    CDS677..4303
    Translation

    Target ORF information:

    RefSeq Version XM_005156668.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156668.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTTCGA AGATGAGCAT
    CCGTCAACAC TGTCACCCAA AAAGAAGCAG CGCAACGGAG GCATGCGAAA TTCACCCAAC
    TGCTCGCCCA AAATGATGAG GCATGATCCT CTACTCATTC CAGGAAATGA GCAGATTGAG
    AGCATGGATG CTAATGTCAA ACGATACGAC TCTACTGGAA TGTTTCACTG GTGTCCATCC
    AAAGAAATCG AGAAGGTCAT TCTGACACGC AGCGAGGCCT CAATGACGGT TTTAAGTGGA
    CATGTCGTAG TCTGCATATT TGGAGATGTC ACGTCAGCTT TAGTGGGGCT TCGAAACTTA
    GTCATGCCTT TGAGAGCAAG CAACTTCCAT TACCATGAGC TAAAGCCCAT TGTGTTTGTG
    GGCTCGTTAG ATTATCTGCG AAGAGAGTGG GAAACCCTTC ACAACTTCCC CAAAGTCTTC
    ATCTTACCTG GTACGCCATT AAGTCGAGCT GATTTAAGGG CCGTCAACAT CAACCTCTGC
    GACATGTGCG TTATCCTGTC AGCCAATCAG AACAATATCG ACGATGCTTC ACTGCAGGAC
    AAGGAATGCA TCTTGGCATC TCTTAACATC AAATCTATGC AGTTTGATGA CAGCATTGGG
    GTCTTGCAGG CTAATTCCCA AGGTTTCACA CCTCCAGGGA TGGATAGGTC ATCTCCTGAT
    AACAGTCCCG TGCACGGCTT AGTGCGGCAG GCATCCGTCA CCACCGGATC AAACATCCCC
    ATCATAACTG AACTGGTGAA CGACTCCAAC GTTCAGTTTT TGGACCAAGA CGACGATGAC
    GACCCAGACA CTGAGCTCTA CCTGACCCAG CCGTTCGCCT GTGGTACCGC ATTCGCCGTC
    AGCGTGTTGG ACTCCCTGAT GAGTGCGACA TACTTCAATG ATAATATCCT CACCCTGATC
    CGGACGCTGG TCACGGGAGG CGCCACGCCG GAGCTGGAGG CCCTGTTGGC TGAGGAGAAC
    GCTCTGAGAG GTGGCTACAG CACGCCGCAG ACGCTGGCTA ACAGGGACAG GTGTCGGGTC
    GCCCAGCTGG CCCTCTACGA TGGGCCCTTC GCAGACTTGG GGGACGGAGG TTGCTATGGA
    GACTTGTTCT GTAAAGCATT AAAAACCTAC AACATGCTGT GCTTTGGAAT CTATAGATTG
    CGAGATGCAC ATTTAGGTGC ACCAAGCCAG TGCACAAAAA GATATGTCAT CACAAACCCG
    CCTTATGAGT TTGAGATGGT GCCCACGGAC CTGATCTTCT GTCTGATGCA GTTCGACCAC
    AACGCGGGCC AGTCCCGAGC GAGCCTGTCC CACTCCTCCC ATTCGTCCCA TTCCTCCAGC
    AAGAAGAGCT CGTCTGTTCA CTCCATCCCA GCCACCAACC GGCAGAACCG CAGCAGCAAA
    GCACGTGAAG CCCGTGACAA ACAGAATGCA ACAAGGATGA ATAGAATGGG CCCAGAAAAG
    AGATGGTTTA CAGATGAAGC AGAAAACGCA TACCCAAGGA ACATTCAAAT CAAGCCTATG
    AGCACTCACA TGGCCAACCA AGTCAATCAG TACAAATCCA CTAGCAGTCT GATTCCACCT
    ATCCGAGAAG TCGAAGACGA ATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18622
    Clone ID Related Accession (Same CDS sequence) XM_005156669.4 , XM_005156669.3
    Accession Version XM_005156669.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X22
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156669.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAACCAAG ATGTCCGTCT ACAAGCGAAT GAAACTGGCA
    TGTTGTTTTG ATTGCGGGCG CTCTGAGCGC GACTGCTCGT GCATGTCAGG CAGTGTACAT
    AGTAACATGG ACAGCCTTCA GAGGGCCTTC CCACTTTCTT CTGTCTCTGT TCATGATTGC
    TCAACCAGTT TACGTGCCTT CGAAGATGAG CATCCGTCAA CACTGTCACC CAAAAAGAAG
    CAGCGCAACG GAGGCATGCG AAATTCACCC AACTGCTCGC CCAAAATGAT GAGGCATGAT
    CCTCTACTCA TTCCAGGAAA TGAGCAGATT GAGAGCATGG ATGCTAATGT CAAACGATAC
    GACTCTACTG GAATGTTTCA CTGGTGTCCA TCCAAAGAAA TCGAGAAGGT CATTCTGACA
    CGCAGCGAGG CCTCAATGAC GGTTTTAAGT GGACATGTCG TAGTCTGCAT ATTTGGAGAT
    GTCACGTCAG CTTTAGTGGG GCTTCGAAAC TTAGTCATGC CTTTGAGAGC AAGCAACTTC
    CATTACCATG AGCTAAAGCC CATTGTGTTT GTGGGCTCGT TAGATTATCT GCGAAGAGAG
    TGGGAAACCC TTCACAACTT CCCCAAAGTC TTCATCTTAC CTGGTACGCC ATTAAGTCGA
    GCTGATTTAA GGGCCGTCAA CATCAACCTC TGCGACATGT GCGTTATCCT GTCAGCCAAT
    CAGAACAATA TCGACGATGC TTCACTGCAG GACAAGGAAT GCATCTTGGC ATCTCTTAAC
    ATCAAATCTA TGCAGTTTGA TGACAGCATT GGGGTCTTGC AGGCTAATTC CCAAGGTTTC
    ACACCTCCAG GGATGGATAG GTCATCTCCT GATAACAGTC CCGTGCACGG CTTAGTGCGG
    CAGGCATCCG TCACCACCGG ATCAAACATC CCCATCATAA CTGAACTGGT GAACGACTCC
    AACGTTCAGT TTTTGGACCA AGACGACGAT GACGACCCAG ACACTGAGCT CTACCTGACC
    CAGCCGTTCG CCTGTGGTAC CGCATTCGCC GTCAGCGTGT TGGACTCCCT GATGAGTGCG
    ACATACTTCA ATGATAATAT CCTCACCCTG ATCCGGACGC TGGTCACGGG AGGCGCCACG
    CCGGAGCTGG AGGCCCTGTT GGCTGAGGAG AACGCTCTGA GAGGTGGCTA CAGCACGCCG
    CAGACGCTGG CTAACAGGGA CAGGTGTCGG GTCGCCCAGC TGGCCCTCTA CGATGGGCCC
    TTCGCAGACT TGGGGGACGG AGGTTGCTAT GGAGACTTGT TCTGTAAAGC ATTAAAAACC
    TACAACATGC TGTGCTTTGG AATCTATAGA TTGCGAGATG CACATTTAGG TGCACCAAGC
    CAGTGCACAA AAAGATATGT CATCACAAAC CCGCCTTATG AGTTTGAGAT GGTGCCCACG
    GACCTGATCT TCTGTCTGAT GCAGTTCGAC CACAACGCGG GCCAGTCCCG AGCGAGCCTG
    TCCCACTCCT CCCATTCGTC CCATTCCTCC AGCAAGAAGA GCTCGTCTGT TCACTCCATC
    CCAGCCACCA ACCGGCAGAA CCGCAGCAGC AAAGCACGTG AAGCCCGTGA CAAACAGAAA
    AAAGAGATGG TTTACAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156726.1
    CDS676..4296
    Translation

    Target ORF information:

    RefSeq Version XM_005156669.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X22, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156669.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAACCAAG ATGTCCGTCT ACAAGCGAAT GAAACTGGCA
    TGTTGTTTTG ATTGCGGGCG CTCTGAGCGC GACTGCTCGT GCATGTCAGG CAGTGTACAT
    AGTAACATGG ACAGCCTTCA GAGGGCCTTC CCACTTTCTT CTGTCTCTGT TCATGATTGC
    TCAACCAGTT TACGTGCCTT CGAAGATGAG CATCCGTCAA CACTGTCACC CAAAAAGAAG
    CAGCGCAACG GAGGCATGCG AAATTCACCC AACTGCTCGC CCAAAATGAT GAGGCATGAT
    CCTCTACTCA TTCCAGGAAA TGAGCAGATT GAGAGCATGG ATGCTAATGT CAAACGATAC
    GACTCTACTG GAATGTTTCA CTGGTGTCCA TCCAAAGAAA TCGAGAAGGT CATTCTGACA
    CGCAGCGAGG CCTCAATGAC GGTTTTAAGT GGACATGTCG TAGTCTGCAT ATTTGGAGAT
    GTCACGTCAG CTTTAGTGGG GCTTCGAAAC TTAGTCATGC CTTTGAGAGC AAGCAACTTC
    CATTACCATG AGCTAAAGCC CATTGTGTTT GTGGGCTCGT TAGATTATCT GCGAAGAGAG
    TGGGAAACCC TTCACAACTT CCCCAAAGTC TTCATCTTAC CTGGTACGCC ATTAAGTCGA
    GCTGATTTAA GGGCCGTCAA CATCAACCTC TGCGACATGT GCGTTATCCT GTCAGCCAAT
    CAGAACAATA TCGACGATGC TTCACTGCAG GACAAGGAAT GCATCTTGGC ATCTCTTAAC
    ATCAAATCTA TGCAGTTTGA TGACAGCATT GGGGTCTTGC AGGCTAATTC CCAAGGTTTC
    ACACCTCCAG GGATGGATAG GTCATCTCCT GATAACAGTC CCGTGCACGG CTTAGTGCGG
    CAGGCATCCG TCACCACCGG ATCAAACATC CCCATCATAA CTGAACTGGT GAACGACTCC
    AACGTTCAGT TTTTGGACCA AGACGACGAT GACGACCCAG ACACTGAGCT CTACCTGACC
    CAGCCGTTCG CCTGTGGTAC CGCATTCGCC GTCAGCGTGT TGGACTCCCT GATGAGTGCG
    ACATACTTCA ATGATAATAT CCTCACCCTG ATCCGGACGC TGGTCACGGG AGGCGCCACG
    CCGGAGCTGG AGGCCCTGTT GGCTGAGGAG AACGCTCTGA GAGGTGGCTA CAGCACGCCG
    CAGACGCTGG CTAACAGGGA CAGGTGTCGG GTCGCCCAGC TGGCCCTCTA CGATGGGCCC
    TTCGCAGACT TGGGGGACGG AGGTTGCTAT GGAGACTTGT TCTGTAAAGC ATTAAAAACC
    TACAACATGC TGTGCTTTGG AATCTATAGA TTGCGAGATG CACATTTAGG TGCACCAAGC
    CAGTGCACAA AAAGATATGT CATCACAAAC CCGCCTTATG AGTTTGAGAT GGTGCCCACG
    GACCTGATCT TCTGTCTGAT GCAGTTCGAC CACAACGCGG GCCAGTCCCG AGCGAGCCTG
    TCCCACTCCT CCCATTCGTC CCATTCCTCC AGCAAGAAGA GCTCGTCTGT TCACTCCATC
    CCAGCCACCA ACCGGCAGAA CCGCAGCAGC AAAGCACGTG AAGCCCGTGA CAAACAGAAA
    AAAGAGATGG TTTACAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18622
    Clone ID Related Accession (Same CDS sequence) XM_005156669.4 , XM_005156669.3
    Accession Version XM_005156669.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3621bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X19
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156669.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAACCAAG ATGTCCGTCT ACAAGCGAAT GAAACTGGCA
    TGTTGTTTTG ATTGCGGGCG CTCTGAGCGC GACTGCTCGT GCATGTCAGG CAGTGTACAT
    AGTAACATGG ACAGCCTTCA GAGGGCCTTC CCACTTTCTT CTGTCTCTGT TCATGATTGC
    TCAACCAGTT TACGTGCCTT CGAAGATGAG CATCCGTCAA CACTGTCACC CAAAAAGAAG
    CAGCGCAACG GAGGCATGCG AAATTCACCC AACTGCTCGC CCAAAATGAT GAGGCATGAT
    CCTCTACTCA TTCCAGGAAA TGAGCAGATT GAGAGCATGG ATGCTAATGT CAAACGATAC
    GACTCTACTG GAATGTTTCA CTGGTGTCCA TCCAAAGAAA TCGAGAAGGT CATTCTGACA
    CGCAGCGAGG CCTCAATGAC GGTTTTAAGT GGACATGTCG TAGTCTGCAT ATTTGGAGAT
    GTCACGTCAG CTTTAGTGGG GCTTCGAAAC TTAGTCATGC CTTTGAGAGC AAGCAACTTC
    CATTACCATG AGCTAAAGCC CATTGTGTTT GTGGGCTCGT TAGATTATCT GCGAAGAGAG
    TGGGAAACCC TTCACAACTT CCCCAAAGTC TTCATCTTAC CTGGTACGCC ATTAAGTCGA
    GCTGATTTAA GGGCCGTCAA CATCAACCTC TGCGACATGT GCGTTATCCT GTCAGCCAAT
    CAGAACAATA TCGACGATGC TTCACTGCAG GACAAGGAAT GCATCTTGGC ATCTCTTAAC
    ATCAAATCTA TGCAGTTTGA TGACAGCATT GGGGTCTTGC AGGCTAATTC CCAAGGTTTC
    ACACCTCCAG GGATGGATAG GTCATCTCCT GATAACAGTC CCGTGCACGG CTTAGTGCGG
    CAGGCATCCG TCACCACCGG ATCAAACATC CCCATCATAA CTGAACTGGT GAACGACTCC
    AACGTTCAGT TTTTGGACCA AGACGACGAT GACGACCCAG ACACTGAGCT CTACCTGACC
    CAGCCGTTCG CCTGTGGTAC CGCATTCGCC GTCAGCGTGT TGGACTCCCT GATGAGTGCG
    ACATACTTCA ATGATAATAT CCTCACCCTG ATCCGGACGC TGGTCACGGG AGGCGCCACG
    CCGGAGCTGG AGGCCCTGTT GGCTGAGGAG AACGCTCTGA GAGGTGGCTA CAGCACGCCG
    CAGACGCTGG CTAACAGGGA CAGGTGTCGG GTCGCCCAGC TGGCCCTCTA CGATGGGCCC
    TTCGCAGACT TGGGGGACGG AGGTTGCTAT GGAGACTTGT TCTGTAAAGC ATTAAAAACC
    TACAACATGC TGTGCTTTGG AATCTATAGA TTGCGAGATG CACATTTAGG TGCACCAAGC
    CAGTGCACAA AAAGATATGT CATCACAAAC CCGCCTTATG AGTTTGAGAT GGTGCCCACG
    GACCTGATCT TCTGTCTGAT GCAGTTCGAC CACAACGCGG GCCAGTCCCG AGCGAGCCTG
    TCCCACTCCT CCCATTCGTC CCATTCCTCC AGCAAGAAGA GCTCGTCTGT TCACTCCATC
    CCAGCCACCA ACCGGCAGAA CCGCAGCAGC AAAGCACGTG AAGCCCGTGA CAAACAGAAA
    AAAGAGATGG TTTACAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156726.1
    CDS653..4273
    Translation

    Target ORF information:

    RefSeq Version XM_005156669.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X19, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156669.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAACCAAG ATGTCCGTCT ACAAGCGAAT GAAACTGGCA
    TGTTGTTTTG ATTGCGGGCG CTCTGAGCGC GACTGCTCGT GCATGTCAGG CAGTGTACAT
    AGTAACATGG ACAGCCTTCA GAGGGCCTTC CCACTTTCTT CTGTCTCTGT TCATGATTGC
    TCAACCAGTT TACGTGCCTT CGAAGATGAG CATCCGTCAA CACTGTCACC CAAAAAGAAG
    CAGCGCAACG GAGGCATGCG AAATTCACCC AACTGCTCGC CCAAAATGAT GAGGCATGAT
    CCTCTACTCA TTCCAGGAAA TGAGCAGATT GAGAGCATGG ATGCTAATGT CAAACGATAC
    GACTCTACTG GAATGTTTCA CTGGTGTCCA TCCAAAGAAA TCGAGAAGGT CATTCTGACA
    CGCAGCGAGG CCTCAATGAC GGTTTTAAGT GGACATGTCG TAGTCTGCAT ATTTGGAGAT
    GTCACGTCAG CTTTAGTGGG GCTTCGAAAC TTAGTCATGC CTTTGAGAGC AAGCAACTTC
    CATTACCATG AGCTAAAGCC CATTGTGTTT GTGGGCTCGT TAGATTATCT GCGAAGAGAG
    TGGGAAACCC TTCACAACTT CCCCAAAGTC TTCATCTTAC CTGGTACGCC ATTAAGTCGA
    GCTGATTTAA GGGCCGTCAA CATCAACCTC TGCGACATGT GCGTTATCCT GTCAGCCAAT
    CAGAACAATA TCGACGATGC TTCACTGCAG GACAAGGAAT GCATCTTGGC ATCTCTTAAC
    ATCAAATCTA TGCAGTTTGA TGACAGCATT GGGGTCTTGC AGGCTAATTC CCAAGGTTTC
    ACACCTCCAG GGATGGATAG GTCATCTCCT GATAACAGTC CCGTGCACGG CTTAGTGCGG
    CAGGCATCCG TCACCACCGG ATCAAACATC CCCATCATAA CTGAACTGGT GAACGACTCC
    AACGTTCAGT TTTTGGACCA AGACGACGAT GACGACCCAG ACACTGAGCT CTACCTGACC
    CAGCCGTTCG CCTGTGGTAC CGCATTCGCC GTCAGCGTGT TGGACTCCCT GATGAGTGCG
    ACATACTTCA ATGATAATAT CCTCACCCTG ATCCGGACGC TGGTCACGGG AGGCGCCACG
    CCGGAGCTGG AGGCCCTGTT GGCTGAGGAG AACGCTCTGA GAGGTGGCTA CAGCACGCCG
    CAGACGCTGG CTAACAGGGA CAGGTGTCGG GTCGCCCAGC TGGCCCTCTA CGATGGGCCC
    TTCGCAGACT TGGGGGACGG AGGTTGCTAT GGAGACTTGT TCTGTAAAGC ATTAAAAACC
    TACAACATGC TGTGCTTTGG AATCTATAGA TTGCGAGATG CACATTTAGG TGCACCAAGC
    CAGTGCACAA AAAGATATGT CATCACAAAC CCGCCTTATG AGTTTGAGAT GGTGCCCACG
    GACCTGATCT TCTGTCTGAT GCAGTTCGAC CACAACGCGG GCCAGTCCCG AGCGAGCCTG
    TCCCACTCCT CCCATTCGTC CCATTCCTCC AGCAAGAAGA GCTCGTCTGT TCACTCCATC
    CCAGCCACCA ACCGGCAGAA CCGCAGCAGC AAAGCACGTG AAGCCCGTGA CAAACAGAAA
    AAAGAGATGG TTTACAGATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18623
    Clone ID Related Accession (Same CDS sequence) XM_005156670.3 , XM_005156670.4
    Accession Version XM_005156670.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X20
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156670.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATCG AAGATGAGCA TCCGTCAACA
    CTGTCACCCA AAAAGAAGCA GCGCAACGGA GGCATGCGAA ATTCACCCAA CTGCTCGCCC
    AAAATGATGA GGCATGATCC TCTACTCATT CCAGGAAATG AGCAGATTGA GAGCATGGAT
    GCTAATGTCA AACGATACGA CTCTACTGGA ATGTTTCACT GGTGTCCATC CAAAGAAATC
    GAGAAGGTCA TTCTGACACG CAGCGAGGCC TCAATGACGG TTTTAAGTGG ACATGTCGTA
    GTCTGCATAT TTGGAGATGT CACGTCAGCT TTAGTGGGGC TTCGAAACTT AGTCATGCCT
    TTGAGAGCAA GCAACTTCCA TTACCATGAG CTAAAGCCCA TTGTGTTTGT GGGCTCGTTA
    GATTATCTGC GAAGAGAGTG GGAAACCCTT CACAACTTCC CCAAAGTCTT CATCTTACCT
    GGTACGCCAT TAAGTCGAGC TGATTTAAGG GCCGTCAACA TCAACCTCTG CGACATGTGC
    GTTATCCTGT CAGCCAATCA GAACAATATC GACGATGCTT CACTGCAGGA CAAGGAATGC
    ATCTTGGCAT CTCTTAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG
    GCTAATTCCC AAGGTTTCAC ACCTCCAGGG ATGGATAGGT CATCTCCTGA TAACAGTCCC
    GTGCACGGCT TAGTGCGGCA GGCATCCGTC ACCACCGGAT CAAACATCCC CATCATAACT
    GAACTGGTGA ACGACTCCAA CGTTCAGTTT TTGGACCAAG ACGACGATGA CGACCCAGAC
    ACTGAGCTCT ACCTGACCCA GCCGTTCGCC TGTGGTACCG CATTCGCCGT CAGCGTGTTG
    GACTCCCTGA TGAGTGCGAC ATACTTCAAT GATAATATCC TCACCCTGAT CCGGACGCTG
    GTCACGGGAG GCGCCACGCC GGAGCTGGAG GCCCTGTTGG CTGAGGAGAA CGCTCTGAGA
    GGTGGCTACA GCACGCCGCA GACGCTGGCT AACAGGGACA GGTGTCGGGT CGCCCAGCTG
    GCCCTCTACG ATGGGCCCTT CGCAGACTTG GGGGACGGAG GTTGCTATGG AGACTTGTTC
    TGTAAAGCAT TAAAAACCTA CAACATGCTG TGCTTTGGAA TCTATAGATT GCGAGATGCA
    CATTTAGGTG CACCAAGCCA GTGCACAAAA AGATATGTCA TCACAAACCC GCCTTATGAG
    TTTGAGATGG TGCCCACGGA CCTGATCTTC TGTCTGATGC AGTTCGACCA CAACGCGGGC
    CAGTCCCGAG CGAGCCTGTC CCACTCCTCC CATTCGTCCC ATTCCTCCAG CAAGAAGAGC
    TCGTCTGTTC ACTCCATCCC AGCCACCAAC CGGCAGAACC GCAGCAGCAA AGCACGTGAA
    GCCCGTGACA AACAGAATGC AACAAGGATG AATAGAATGG GCCCAGAAAA GAGATGGTTT
    ACAGATGAAG CAGAAAACGC ATACCCAAGG AACATTCAAA TCAAGCCTAT GAGCACTCAC
    ATGGCCAACC AAGTCAATCA GTACAAATCC ACTAGCAGTC TGATTCCACC TATCCGAGAA
    GTCGAAGACG AATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156727.1
    CDS658..4275
    Translation

    Target ORF information:

    RefSeq Version XM_005156670.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X20, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156670.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATCG AAGATGAGCA TCCGTCAACA
    CTGTCACCCA AAAAGAAGCA GCGCAACGGA GGCATGCGAA ATTCACCCAA CTGCTCGCCC
    AAAATGATGA GGCATGATCC TCTACTCATT CCAGGAAATG AGCAGATTGA GAGCATGGAT
    GCTAATGTCA AACGATACGA CTCTACTGGA ATGTTTCACT GGTGTCCATC CAAAGAAATC
    GAGAAGGTCA TTCTGACACG CAGCGAGGCC TCAATGACGG TTTTAAGTGG ACATGTCGTA
    GTCTGCATAT TTGGAGATGT CACGTCAGCT TTAGTGGGGC TTCGAAACTT AGTCATGCCT
    TTGAGAGCAA GCAACTTCCA TTACCATGAG CTAAAGCCCA TTGTGTTTGT GGGCTCGTTA
    GATTATCTGC GAAGAGAGTG GGAAACCCTT CACAACTTCC CCAAAGTCTT CATCTTACCT
    GGTACGCCAT TAAGTCGAGC TGATTTAAGG GCCGTCAACA TCAACCTCTG CGACATGTGC
    GTTATCCTGT CAGCCAATCA GAACAATATC GACGATGCTT CACTGCAGGA CAAGGAATGC
    ATCTTGGCAT CTCTTAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG
    GCTAATTCCC AAGGTTTCAC ACCTCCAGGG ATGGATAGGT CATCTCCTGA TAACAGTCCC
    GTGCACGGCT TAGTGCGGCA GGCATCCGTC ACCACCGGAT CAAACATCCC CATCATAACT
    GAACTGGTGA ACGACTCCAA CGTTCAGTTT TTGGACCAAG ACGACGATGA CGACCCAGAC
    ACTGAGCTCT ACCTGACCCA GCCGTTCGCC TGTGGTACCG CATTCGCCGT CAGCGTGTTG
    GACTCCCTGA TGAGTGCGAC ATACTTCAAT GATAATATCC TCACCCTGAT CCGGACGCTG
    GTCACGGGAG GCGCCACGCC GGAGCTGGAG GCCCTGTTGG CTGAGGAGAA CGCTCTGAGA
    GGTGGCTACA GCACGCCGCA GACGCTGGCT AACAGGGACA GGTGTCGGGT CGCCCAGCTG
    GCCCTCTACG ATGGGCCCTT CGCAGACTTG GGGGACGGAG GTTGCTATGG AGACTTGTTC
    TGTAAAGCAT TAAAAACCTA CAACATGCTG TGCTTTGGAA TCTATAGATT GCGAGATGCA
    CATTTAGGTG CACCAAGCCA GTGCACAAAA AGATATGTCA TCACAAACCC GCCTTATGAG
    TTTGAGATGG TGCCCACGGA CCTGATCTTC TGTCTGATGC AGTTCGACCA CAACGCGGGC
    CAGTCCCGAG CGAGCCTGTC CCACTCCTCC CATTCGTCCC ATTCCTCCAG CAAGAAGAGC
    TCGTCTGTTC ACTCCATCCC AGCCACCAAC CGGCAGAACC GCAGCAGCAA AGCACGTGAA
    GCCCGTGACA AACAGAATGC AACAAGGATG AATAGAATGG GCCCAGAAAA GAGATGGTTT
    ACAGATGAAG CAGAAAACGC ATACCCAAGG AACATTCAAA TCAAGCCTAT GAGCACTCAC
    ATGGCCAACC AAGTCAATCA GTACAAATCC ACTAGCAGTC TGATTCCACC TATCCGAGAA
    GTCGAAGACG AATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18623
    Clone ID Related Accession (Same CDS sequence) XM_005156670.3 , XM_005156670.4
    Accession Version XM_005156670.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X23
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156670.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATCG AAGATGAGCA TCCGTCAACA
    CTGTCACCCA AAAAGAAGCA GCGCAACGGA GGCATGCGAA ATTCACCCAA CTGCTCGCCC
    AAAATGATGA GGCATGATCC TCTACTCATT CCAGGAAATG AGCAGATTGA GAGCATGGAT
    GCTAATGTCA AACGATACGA CTCTACTGGA ATGTTTCACT GGTGTCCATC CAAAGAAATC
    GAGAAGGTCA TTCTGACACG CAGCGAGGCC TCAATGACGG TTTTAAGTGG ACATGTCGTA
    GTCTGCATAT TTGGAGATGT CACGTCAGCT TTAGTGGGGC TTCGAAACTT AGTCATGCCT
    TTGAGAGCAA GCAACTTCCA TTACCATGAG CTAAAGCCCA TTGTGTTTGT GGGCTCGTTA
    GATTATCTGC GAAGAGAGTG GGAAACCCTT CACAACTTCC CCAAAGTCTT CATCTTACCT
    GGTACGCCAT TAAGTCGAGC TGATTTAAGG GCCGTCAACA TCAACCTCTG CGACATGTGC
    GTTATCCTGT CAGCCAATCA GAACAATATC GACGATGCTT CACTGCAGGA CAAGGAATGC
    ATCTTGGCAT CTCTTAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG
    GCTAATTCCC AAGGTTTCAC ACCTCCAGGG ATGGATAGGT CATCTCCTGA TAACAGTCCC
    GTGCACGGCT TAGTGCGGCA GGCATCCGTC ACCACCGGAT CAAACATCCC CATCATAACT
    GAACTGGTGA ACGACTCCAA CGTTCAGTTT TTGGACCAAG ACGACGATGA CGACCCAGAC
    ACTGAGCTCT ACCTGACCCA GCCGTTCGCC TGTGGTACCG CATTCGCCGT CAGCGTGTTG
    GACTCCCTGA TGAGTGCGAC ATACTTCAAT GATAATATCC TCACCCTGAT CCGGACGCTG
    GTCACGGGAG GCGCCACGCC GGAGCTGGAG GCCCTGTTGG CTGAGGAGAA CGCTCTGAGA
    GGTGGCTACA GCACGCCGCA GACGCTGGCT AACAGGGACA GGTGTCGGGT CGCCCAGCTG
    GCCCTCTACG ATGGGCCCTT CGCAGACTTG GGGGACGGAG GTTGCTATGG AGACTTGTTC
    TGTAAAGCAT TAAAAACCTA CAACATGCTG TGCTTTGGAA TCTATAGATT GCGAGATGCA
    CATTTAGGTG CACCAAGCCA GTGCACAAAA AGATATGTCA TCACAAACCC GCCTTATGAG
    TTTGAGATGG TGCCCACGGA CCTGATCTTC TGTCTGATGC AGTTCGACCA CAACGCGGGC
    CAGTCCCGAG CGAGCCTGTC CCACTCCTCC CATTCGTCCC ATTCCTCCAG CAAGAAGAGC
    TCGTCTGTTC ACTCCATCCC AGCCACCAAC CGGCAGAACC GCAGCAGCAA AGCACGTGAA
    GCCCGTGACA AACAGAATGC AACAAGGATG AATAGAATGG GCCCAGAAAA GAGATGGTTT
    ACAGATGAAG CAGAAAACGC ATACCCAAGG AACATTCAAA TCAAGCCTAT GAGCACTCAC
    ATGGCCAACC AAGTCAATCA GTACAAATCC ACTAGCAGTC TGATTCCACC TATCCGAGAA
    GTCGAAGACG AATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156727.1
    CDS677..4294
    Translation

    Target ORF information:

    RefSeq Version XM_005156670.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X23, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156670.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATCG AAGATGAGCA TCCGTCAACA
    CTGTCACCCA AAAAGAAGCA GCGCAACGGA GGCATGCGAA ATTCACCCAA CTGCTCGCCC
    AAAATGATGA GGCATGATCC TCTACTCATT CCAGGAAATG AGCAGATTGA GAGCATGGAT
    GCTAATGTCA AACGATACGA CTCTACTGGA ATGTTTCACT GGTGTCCATC CAAAGAAATC
    GAGAAGGTCA TTCTGACACG CAGCGAGGCC TCAATGACGG TTTTAAGTGG ACATGTCGTA
    GTCTGCATAT TTGGAGATGT CACGTCAGCT TTAGTGGGGC TTCGAAACTT AGTCATGCCT
    TTGAGAGCAA GCAACTTCCA TTACCATGAG CTAAAGCCCA TTGTGTTTGT GGGCTCGTTA
    GATTATCTGC GAAGAGAGTG GGAAACCCTT CACAACTTCC CCAAAGTCTT CATCTTACCT
    GGTACGCCAT TAAGTCGAGC TGATTTAAGG GCCGTCAACA TCAACCTCTG CGACATGTGC
    GTTATCCTGT CAGCCAATCA GAACAATATC GACGATGCTT CACTGCAGGA CAAGGAATGC
    ATCTTGGCAT CTCTTAACAT CAAATCTATG CAGTTTGATG ACAGCATTGG GGTCTTGCAG
    GCTAATTCCC AAGGTTTCAC ACCTCCAGGG ATGGATAGGT CATCTCCTGA TAACAGTCCC
    GTGCACGGCT TAGTGCGGCA GGCATCCGTC ACCACCGGAT CAAACATCCC CATCATAACT
    GAACTGGTGA ACGACTCCAA CGTTCAGTTT TTGGACCAAG ACGACGATGA CGACCCAGAC
    ACTGAGCTCT ACCTGACCCA GCCGTTCGCC TGTGGTACCG CATTCGCCGT CAGCGTGTTG
    GACTCCCTGA TGAGTGCGAC ATACTTCAAT GATAATATCC TCACCCTGAT CCGGACGCTG
    GTCACGGGAG GCGCCACGCC GGAGCTGGAG GCCCTGTTGG CTGAGGAGAA CGCTCTGAGA
    GGTGGCTACA GCACGCCGCA GACGCTGGCT AACAGGGACA GGTGTCGGGT CGCCCAGCTG
    GCCCTCTACG ATGGGCCCTT CGCAGACTTG GGGGACGGAG GTTGCTATGG AGACTTGTTC
    TGTAAAGCAT TAAAAACCTA CAACATGCTG TGCTTTGGAA TCTATAGATT GCGAGATGCA
    CATTTAGGTG CACCAAGCCA GTGCACAAAA AGATATGTCA TCACAAACCC GCCTTATGAG
    TTTGAGATGG TGCCCACGGA CCTGATCTTC TGTCTGATGC AGTTCGACCA CAACGCGGGC
    CAGTCCCGAG CGAGCCTGTC CCACTCCTCC CATTCGTCCC ATTCCTCCAG CAAGAAGAGC
    TCGTCTGTTC ACTCCATCCC AGCCACCAAC CGGCAGAACC GCAGCAGCAA AGCACGTGAA
    GCCCGTGACA AACAGAATGC AACAAGGATG AATAGAATGG GCCCAGAAAA GAGATGGTTT
    ACAGATGAAG CAGAAAACGC ATACCCAAGG AACATTCAAA TCAAGCCTAT GAGCACTCAC
    ATGGCCAACC AAGTCAATCA GTACAAATCC ACTAGCAGTC TGATTCCACC TATCCGAGAA
    GTCGAAGACG AATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18624
    Clone ID Related Accession (Same CDS sequence) XM_005156671.4 , XM_005156671.3
    Accession Version XM_005156671.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3615bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X25
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005156671.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATGACA TATTTCGAAG ATGAGCATCC GTCAACACTG
    TCACCCAAAA AGAAGCAGCG CAACGGAGGC ATGCGAAATT CACCCAACTG CTCGCCCAAA
    ATGATGAGGC ATGATCCTCT ACTCATTCCA GGAAATGAGC AGATTGAGAG CATGGATGCT
    AATGTCAAAC GATACGACTC TACTGGAATG TTTCACTGGT GTCCATCCAA AGAAATCGAG
    AAGGTCATTC TGACACGCAG CGAGGCCTCA ATGACGGTTT TAAGTGGACA TGTCGTAGTC
    TGCATATTTG GAGATGTCAC GTCAGCTTTA GTGGGGCTTC GAAACTTAGT CATGCCTTTG
    AGAGCAAGCA ACTTCCATTA CCATGAGCTA AAGCCCATTG TGTTTGTGGG CTCGTTAGAT
    TATCTGCGAA GAGAGTGGGA AACCCTTCAC AACTTCCCCA AAGTCTTCAT CTTACCTGGT
    ACGCCATTAA GTCGAGCTGA TTTAAGGGCC GTCAACATCA ACCTCTGCGA CATGTGCGTT
    ATCCTGTCAG CCAATCAGAA CAATATCGAC GATGCTTCAC TGCAGGACAA GGAATGCATC
    TTGGCATCTC TTAACATCAA ATCTATGCAG TTTGATGACA GCATTGGGGT CTTGCAGGCT
    AATTCCCAAG GTTTCACACC TCCAGGGATG GATAGGTCAT CTCCTGATAA CAGTCCCGTG
    CACGGCTTAG TGCGGCAGGC ATCCGTCACC ACCGGATCAA ACATCCCCAT CATAACTGAA
    CTGGTGAACG ACTCCAACGT TCAGTTTTTG GACCAAGACG ACGATGACGA CCCAGACACT
    GAGCTCTACC TGACCCAGCC GTTCGCCTGT GGTACCGCAT TCGCCGTCAG CGTGTTGGAC
    TCCCTGATGA GTGCGACATA CTTCAATGAT AATATCCTCA CCCTGATCCG GACGCTGGTC
    ACGGGAGGCG CCACGCCGGA GCTGGAGGCC CTGTTGGCTG AGGAGAACGC TCTGAGAGGT
    GGCTACAGCA CGCCGCAGAC GCTGGCTAAC AGGGACAGGT GTCGGGTCGC CCAGCTGGCC
    CTCTACGATG GGCCCTTCGC AGACTTGGGG GACGGAGGTT GCTATGGAGA CTTGTTCTGT
    AAAGCATTAA AAACCTACAA CATGCTGTGC TTTGGAATCT ATAGATTGCG AGATGCACAT
    TTAGGTGCAC CAAGCCAGTG CACAAAAAGA TATGTCATCA CAAACCCGCC TTATGAGTTT
    GAGATGGTGC CCACGGACCT GATCTTCTGT CTGATGCAGT TCGACCACAA CGCGGGCCAG
    TCCCGAGCGA GCCTGTCCCA CTCCTCCCAT TCGTCCCATT CCTCCAGCAA GAAGAGCTCG
    TCTGTTCACT CCATCCCAGC CACCAACCGG CAGAACCGCA GCAGCAAAGC ACGTGAAGCC
    CGTGACAAAC AGAATGCAAC AAGGATGAAT AGAATGGGCC CAGAAAAGAG ATGGTTTACA
    GATGAAGCAG AAAACGCATA CCCAAGGAAC ATTCAAATCA AGCCTATGAG CACTCACATG
    GCCAACCAAG TCAATCAGTA CAAATCCACT AGCAGTCTGA TTCCACCTAT CCGAGAAGTC
    GAAGACGAAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156728.1
    CDS676..4290
    Translation

    Target ORF information:

    RefSeq Version XM_005156671.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X25, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156671.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATGACA TATTTCGAAG ATGAGCATCC GTCAACACTG
    TCACCCAAAA AGAAGCAGCG CAACGGAGGC ATGCGAAATT CACCCAACTG CTCGCCCAAA
    ATGATGAGGC ATGATCCTCT ACTCATTCCA GGAAATGAGC AGATTGAGAG CATGGATGCT
    AATGTCAAAC GATACGACTC TACTGGAATG TTTCACTGGT GTCCATCCAA AGAAATCGAG
    AAGGTCATTC TGACACGCAG CGAGGCCTCA ATGACGGTTT TAAGTGGACA TGTCGTAGTC
    TGCATATTTG GAGATGTCAC GTCAGCTTTA GTGGGGCTTC GAAACTTAGT CATGCCTTTG
    AGAGCAAGCA ACTTCCATTA CCATGAGCTA AAGCCCATTG TGTTTGTGGG CTCGTTAGAT
    TATCTGCGAA GAGAGTGGGA AACCCTTCAC AACTTCCCCA AAGTCTTCAT CTTACCTGGT
    ACGCCATTAA GTCGAGCTGA TTTAAGGGCC GTCAACATCA ACCTCTGCGA CATGTGCGTT
    ATCCTGTCAG CCAATCAGAA CAATATCGAC GATGCTTCAC TGCAGGACAA GGAATGCATC
    TTGGCATCTC TTAACATCAA ATCTATGCAG TTTGATGACA GCATTGGGGT CTTGCAGGCT
    AATTCCCAAG GTTTCACACC TCCAGGGATG GATAGGTCAT CTCCTGATAA CAGTCCCGTG
    CACGGCTTAG TGCGGCAGGC ATCCGTCACC ACCGGATCAA ACATCCCCAT CATAACTGAA
    CTGGTGAACG ACTCCAACGT TCAGTTTTTG GACCAAGACG ACGATGACGA CCCAGACACT
    GAGCTCTACC TGACCCAGCC GTTCGCCTGT GGTACCGCAT TCGCCGTCAG CGTGTTGGAC
    TCCCTGATGA GTGCGACATA CTTCAATGAT AATATCCTCA CCCTGATCCG GACGCTGGTC
    ACGGGAGGCG CCACGCCGGA GCTGGAGGCC CTGTTGGCTG AGGAGAACGC TCTGAGAGGT
    GGCTACAGCA CGCCGCAGAC GCTGGCTAAC AGGGACAGGT GTCGGGTCGC CCAGCTGGCC
    CTCTACGATG GGCCCTTCGC AGACTTGGGG GACGGAGGTT GCTATGGAGA CTTGTTCTGT
    AAAGCATTAA AAACCTACAA CATGCTGTGC TTTGGAATCT ATAGATTGCG AGATGCACAT
    TTAGGTGCAC CAAGCCAGTG CACAAAAAGA TATGTCATCA CAAACCCGCC TTATGAGTTT
    GAGATGGTGC CCACGGACCT GATCTTCTGT CTGATGCAGT TCGACCACAA CGCGGGCCAG
    TCCCGAGCGA GCCTGTCCCA CTCCTCCCAT TCGTCCCATT CCTCCAGCAA GAAGAGCTCG
    TCTGTTCACT CCATCCCAGC CACCAACCGG CAGAACCGCA GCAGCAAAGC ACGTGAAGCC
    CGTGACAAAC AGAATGCAAC AAGGATGAAT AGAATGGGCC CAGAAAAGAG ATGGTTTACA
    GATGAAGCAG AAAACGCATA CCCAAGGAAC ATTCAAATCA AGCCTATGAG CACTCACATG
    GCCAACCAAG TCAATCAGTA CAAATCCACT AGCAGTCTGA TTCCACCTAT CCGAGAAGTC
    GAAGACGAAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa18624
    Clone ID Related Accession (Same CDS sequence) XM_005156671.4 , XM_005156671.3
    Accession Version XM_005156671.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3615bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X21
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005156671.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATGACA TATTTCGAAG ATGAGCATCC GTCAACACTG
    TCACCCAAAA AGAAGCAGCG CAACGGAGGC ATGCGAAATT CACCCAACTG CTCGCCCAAA
    ATGATGAGGC ATGATCCTCT ACTCATTCCA GGAAATGAGC AGATTGAGAG CATGGATGCT
    AATGTCAAAC GATACGACTC TACTGGAATG TTTCACTGGT GTCCATCCAA AGAAATCGAG
    AAGGTCATTC TGACACGCAG CGAGGCCTCA ATGACGGTTT TAAGTGGACA TGTCGTAGTC
    TGCATATTTG GAGATGTCAC GTCAGCTTTA GTGGGGCTTC GAAACTTAGT CATGCCTTTG
    AGAGCAAGCA ACTTCCATTA CCATGAGCTA AAGCCCATTG TGTTTGTGGG CTCGTTAGAT
    TATCTGCGAA GAGAGTGGGA AACCCTTCAC AACTTCCCCA AAGTCTTCAT CTTACCTGGT
    ACGCCATTAA GTCGAGCTGA TTTAAGGGCC GTCAACATCA ACCTCTGCGA CATGTGCGTT
    ATCCTGTCAG CCAATCAGAA CAATATCGAC GATGCTTCAC TGCAGGACAA GGAATGCATC
    TTGGCATCTC TTAACATCAA ATCTATGCAG TTTGATGACA GCATTGGGGT CTTGCAGGCT
    AATTCCCAAG GTTTCACACC TCCAGGGATG GATAGGTCAT CTCCTGATAA CAGTCCCGTG
    CACGGCTTAG TGCGGCAGGC ATCCGTCACC ACCGGATCAA ACATCCCCAT CATAACTGAA
    CTGGTGAACG ACTCCAACGT TCAGTTTTTG GACCAAGACG ACGATGACGA CCCAGACACT
    GAGCTCTACC TGACCCAGCC GTTCGCCTGT GGTACCGCAT TCGCCGTCAG CGTGTTGGAC
    TCCCTGATGA GTGCGACATA CTTCAATGAT AATATCCTCA CCCTGATCCG GACGCTGGTC
    ACGGGAGGCG CCACGCCGGA GCTGGAGGCC CTGTTGGCTG AGGAGAACGC TCTGAGAGGT
    GGCTACAGCA CGCCGCAGAC GCTGGCTAAC AGGGACAGGT GTCGGGTCGC CCAGCTGGCC
    CTCTACGATG GGCCCTTCGC AGACTTGGGG GACGGAGGTT GCTATGGAGA CTTGTTCTGT
    AAAGCATTAA AAACCTACAA CATGCTGTGC TTTGGAATCT ATAGATTGCG AGATGCACAT
    TTAGGTGCAC CAAGCCAGTG CACAAAAAGA TATGTCATCA CAAACCCGCC TTATGAGTTT
    GAGATGGTGC CCACGGACCT GATCTTCTGT CTGATGCAGT TCGACCACAA CGCGGGCCAG
    TCCCGAGCGA GCCTGTCCCA CTCCTCCCAT TCGTCCCATT CCTCCAGCAA GAAGAGCTCG
    TCTGTTCACT CCATCCCAGC CACCAACCGG CAGAACCGCA GCAGCAAAGC ACGTGAAGCC
    CGTGACAAAC AGAATGCAAC AAGGATGAAT AGAATGGGCC CAGAAAAGAG ATGGTTTACA
    GATGAAGCAG AAAACGCATA CCCAAGGAAC ATTCAAATCA AGCCTATGAG CACTCACATG
    GCCAACCAAG TCAATCAGTA CAAATCCACT AGCAGTCTGA TTCCACCTAT CCGAGAAGTC
    GAAGACGAAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005156728.1
    CDS656..4270
    Translation

    Target ORF information:

    RefSeq Version XM_005156671.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X21, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005156671.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATGACA TATTTCGAAG ATGAGCATCC GTCAACACTG
    TCACCCAAAA AGAAGCAGCG CAACGGAGGC ATGCGAAATT CACCCAACTG CTCGCCCAAA
    ATGATGAGGC ATGATCCTCT ACTCATTCCA GGAAATGAGC AGATTGAGAG CATGGATGCT
    AATGTCAAAC GATACGACTC TACTGGAATG TTTCACTGGT GTCCATCCAA AGAAATCGAG
    AAGGTCATTC TGACACGCAG CGAGGCCTCA ATGACGGTTT TAAGTGGACA TGTCGTAGTC
    TGCATATTTG GAGATGTCAC GTCAGCTTTA GTGGGGCTTC GAAACTTAGT CATGCCTTTG
    AGAGCAAGCA ACTTCCATTA CCATGAGCTA AAGCCCATTG TGTTTGTGGG CTCGTTAGAT
    TATCTGCGAA GAGAGTGGGA AACCCTTCAC AACTTCCCCA AAGTCTTCAT CTTACCTGGT
    ACGCCATTAA GTCGAGCTGA TTTAAGGGCC GTCAACATCA ACCTCTGCGA CATGTGCGTT
    ATCCTGTCAG CCAATCAGAA CAATATCGAC GATGCTTCAC TGCAGGACAA GGAATGCATC
    TTGGCATCTC TTAACATCAA ATCTATGCAG TTTGATGACA GCATTGGGGT CTTGCAGGCT
    AATTCCCAAG GTTTCACACC TCCAGGGATG GATAGGTCAT CTCCTGATAA CAGTCCCGTG
    CACGGCTTAG TGCGGCAGGC ATCCGTCACC ACCGGATCAA ACATCCCCAT CATAACTGAA
    CTGGTGAACG ACTCCAACGT TCAGTTTTTG GACCAAGACG ACGATGACGA CCCAGACACT
    GAGCTCTACC TGACCCAGCC GTTCGCCTGT GGTACCGCAT TCGCCGTCAG CGTGTTGGAC
    TCCCTGATGA GTGCGACATA CTTCAATGAT AATATCCTCA CCCTGATCCG GACGCTGGTC
    ACGGGAGGCG CCACGCCGGA GCTGGAGGCC CTGTTGGCTG AGGAGAACGC TCTGAGAGGT
    GGCTACAGCA CGCCGCAGAC GCTGGCTAAC AGGGACAGGT GTCGGGTCGC CCAGCTGGCC
    CTCTACGATG GGCCCTTCGC AGACTTGGGG GACGGAGGTT GCTATGGAGA CTTGTTCTGT
    AAAGCATTAA AAACCTACAA CATGCTGTGC TTTGGAATCT ATAGATTGCG AGATGCACAT
    TTAGGTGCAC CAAGCCAGTG CACAAAAAGA TATGTCATCA CAAACCCGCC TTATGAGTTT
    GAGATGGTGC CCACGGACCT GATCTTCTGT CTGATGCAGT TCGACCACAA CGCGGGCCAG
    TCCCGAGCGA GCCTGTCCCA CTCCTCCCAT TCGTCCCATT CCTCCAGCAA GAAGAGCTCG
    TCTGTTCACT CCATCCCAGC CACCAACCGG CAGAACCGCA GCAGCAAAGC ACGTGAAGCC
    CGTGACAAAC AGAATGCAAC AAGGATGAAT AGAATGGGCC CAGAAAAGAG ATGGTTTACA
    GATGAAGCAG AAAACGCATA CCCAAGGAAC ATTCAAATCA AGCCTATGAG CACTCACATG
    GCCAACCAAG TCAATCAGTA CAAATCCACT AGCAGTCTGA TTCCACCTAT CCGAGAAGTC
    GAAGACGAAT GCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45445
    Clone ID Related Accession (Same CDS sequence) XM_009307242.2 , XM_009307242.3
    Accession Version XM_009307242.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307242.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT CCAAATATAA GTTTAACGGA
    TATGTCAGAT CACTCGAAGA TGAGCATCCG TCAACACTGT CACCCAAAAA GAAGCAGCGC
    AACGGAGGCA TGCGAAATTC ACCCAACTGC TCGCCCAAAA TGATGAGGCA TGATCCTCTA
    CTCATTCCAG GAAATGAGCA GATTGAGAGC ATGGATGCTA ATGTCAAACG ATACGACTCT
    ACTGGAATGT TTCACTGGTG TCCATCCAAA GAAATCGAGA AGGTCATTCT GACACGCAGC
    GAGGCCTCAA TGACGGTTTT AAGTGGACAT GTCGTAGTCT GCATATTTGG AGATGTCACG
    TCAGCTTTAG TGGGGCTTCG AAACTTAGTC ATGCCTTTGA GAGCAAGCAA CTTCCATTAC
    CATGAGCTAA AGCCCATTGT GTTTGTGGGC TCGTTAGATT ATCTGCGAAG AGAGTGGGAA
    ACCCTTCACA ACTTCCCCAA AGTCTTCATC TTACCTGGTA CGCCATTAAG TCGAGCTGAT
    TTAAGGGCCG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAC
    AATATCGACG ATGCTTCACT GCAGGACAAG GAATGCATCT TGGCATCTCT TAACATCAAA
    TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG TTTCACACCT
    CCAGGGATGG ATAGGTCATC TCCTGATAAC AGTCCCGTGC ACGGCTTAGT GCGGCAGGCA
    TCCGTCACCA CCGGATCAAA CATCCCCATC ATAACTGAAC TGGCTAAGCC TGGCAAACTA
    CTGCCTTTGG TTTCATTCAG TCAGGAAAAA AACAGTGGAA CCAACATACA AATGATAACA
    GAACTGGTGA ACGACTCCAA CGTTCAGTTT TTGGACCAAG ACGACGATGA CGACCCAGAC
    ACTGAGCTCT ACCTGACCCA GCCGTTCGCC TGTGGTACCG CATTCGCCGT CAGCGTGTTG
    GACTCCCTGA TGAGTGCGAC ATACTTCAAT GATAATATCC TCACCCTGAT CCGGACGCTG
    GTCACGGGAG GCGCCACGCC GGAGCTGGAG GCCCTGTTGG CTGAGGAGAA CGCTCTGAGA
    GGTGGCTACA GCACGCCGCA GACGCTGGCT AACAGGGACA GGTGTCGGGT CGCCCAGCTG
    GCCCTCTACG ATGGGCCCTT CGCAGACTTG GGGGACGGAG GTTGCTATGG AGACTTGTTC
    TGTAAAGCAT TAAAAACCTA CAACATGCTG TGCTTTGGAA TCTATAGATT GCGAGATGCA
    CATTTAGGTG CACCAAGCCA GTGCACAAAA AGATATGTCA TCACAAACCC GCCTTATGAG
    TTTGAGATGG TGCCCACGGA CCTGATCTTC TGTCTGATGC AGTTCGACCA CAACGCGGGC
    CAGTCCCGAG CGAGCCTGTC CCACTCCTCC CATTCGTCCC ATTCCTCCAG CAAGAAGAGC
    TCGTCTGTTC ACTCCATCCC AGCCACCAAC CGGCAGAACC GCAGCAGCAA AGCACGTGAA
    GCCCGTGACA AACAGAATGC AACAAGGATG AATAGAATGG GCCCAGAAAA GAGATGGTTT
    ACAGATGAAG CAGAAAACGC ATACCCAAGG AACATTCAAA TCAAGCCTAT GAGCACTCAC
    ATGGCCAACC AAGTCAATCA GTACAAATCC ACTAGCAGTC TGATTCCACC TATCCGAGAA
    GTCGAAGACG AATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305517.1
    CDS661..4578
    Translation

    Target ORF information:

    RefSeq Version XM_009307242.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307242.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT CCAAATATAA GTTTAACGGA
    TATGTCAGAT CACTCGAAGA TGAGCATCCG TCAACACTGT CACCCAAAAA GAAGCAGCGC
    AACGGAGGCA TGCGAAATTC ACCCAACTGC TCGCCCAAAA TGATGAGGCA TGATCCTCTA
    CTCATTCCAG GAAATGAGCA GATTGAGAGC ATGGATGCTA ATGTCAAACG ATACGACTCT
    ACTGGAATGT TTCACTGGTG TCCATCCAAA GAAATCGAGA AGGTCATTCT GACACGCAGC
    GAGGCCTCAA TGACGGTTTT AAGTGGACAT GTCGTAGTCT GCATATTTGG AGATGTCACG
    TCAGCTTTAG TGGGGCTTCG AAACTTAGTC ATGCCTTTGA GAGCAAGCAA CTTCCATTAC
    CATGAGCTAA AGCCCATTGT GTTTGTGGGC TCGTTAGATT ATCTGCGAAG AGAGTGGGAA
    ACCCTTCACA ACTTCCCCAA AGTCTTCATC TTACCTGGTA CGCCATTAAG TCGAGCTGAT
    TTAAGGGCCG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAC
    AATATCGACG ATGCTTCACT GCAGGACAAG GAATGCATCT TGGCATCTCT TAACATCAAA
    TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG TTTCACACCT
    CCAGGGATGG ATAGGTCATC TCCTGATAAC AGTCCCGTGC ACGGCTTAGT GCGGCAGGCA
    TCCGTCACCA CCGGATCAAA CATCCCCATC ATAACTGAAC TGGCTAAGCC TGGCAAACTA
    CTGCCTTTGG TTTCATTCAG TCAGGAAAAA AACAGTGGAA CCAACATACA AATGATAACA
    GAACTGGTGA ACGACTCCAA CGTTCAGTTT TTGGACCAAG ACGACGATGA CGACCCAGAC
    ACTGAGCTCT ACCTGACCCA GCCGTTCGCC TGTGGTACCG CATTCGCCGT CAGCGTGTTG
    GACTCCCTGA TGAGTGCGAC ATACTTCAAT GATAATATCC TCACCCTGAT CCGGACGCTG
    GTCACGGGAG GCGCCACGCC GGAGCTGGAG GCCCTGTTGG CTGAGGAGAA CGCTCTGAGA
    GGTGGCTACA GCACGCCGCA GACGCTGGCT AACAGGGACA GGTGTCGGGT CGCCCAGCTG
    GCCCTCTACG ATGGGCCCTT CGCAGACTTG GGGGACGGAG GTTGCTATGG AGACTTGTTC
    TGTAAAGCAT TAAAAACCTA CAACATGCTG TGCTTTGGAA TCTATAGATT GCGAGATGCA
    CATTTAGGTG CACCAAGCCA GTGCACAAAA AGATATGTCA TCACAAACCC GCCTTATGAG
    TTTGAGATGG TGCCCACGGA CCTGATCTTC TGTCTGATGC AGTTCGACCA CAACGCGGGC
    CAGTCCCGAG CGAGCCTGTC CCACTCCTCC CATTCGTCCC ATTCCTCCAG CAAGAAGAGC
    TCGTCTGTTC ACTCCATCCC AGCCACCAAC CGGCAGAACC GCAGCAGCAA AGCACGTGAA
    GCCCGTGACA AACAGAATGC AACAAGGATG AATAGAATGG GCCCAGAAAA GAGATGGTTT
    ACAGATGAAG CAGAAAACGC ATACCCAAGG AACATTCAAA TCAAGCCTAT GAGCACTCAC
    ATGGCCAACC AAGTCAATCA GTACAAATCC ACTAGCAGTC TGATTCCACC TATCCGAGAA
    GTCGAAGACG AATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45445
    Clone ID Related Accession (Same CDS sequence) XM_009307242.2 , XM_009307242.3
    Accession Version XM_009307242.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3918bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307242.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT CCAAATATAA GTTTAACGGA
    TATGTCAGAT CACTCGAAGA TGAGCATCCG TCAACACTGT CACCCAAAAA GAAGCAGCGC
    AACGGAGGCA TGCGAAATTC ACCCAACTGC TCGCCCAAAA TGATGAGGCA TGATCCTCTA
    CTCATTCCAG GAAATGAGCA GATTGAGAGC ATGGATGCTA ATGTCAAACG ATACGACTCT
    ACTGGAATGT TTCACTGGTG TCCATCCAAA GAAATCGAGA AGGTCATTCT GACACGCAGC
    GAGGCCTCAA TGACGGTTTT AAGTGGACAT GTCGTAGTCT GCATATTTGG AGATGTCACG
    TCAGCTTTAG TGGGGCTTCG AAACTTAGTC ATGCCTTTGA GAGCAAGCAA CTTCCATTAC
    CATGAGCTAA AGCCCATTGT GTTTGTGGGC TCGTTAGATT ATCTGCGAAG AGAGTGGGAA
    ACCCTTCACA ACTTCCCCAA AGTCTTCATC TTACCTGGTA CGCCATTAAG TCGAGCTGAT
    TTAAGGGCCG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAC
    AATATCGACG ATGCTTCACT GCAGGACAAG GAATGCATCT TGGCATCTCT TAACATCAAA
    TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG TTTCACACCT
    CCAGGGATGG ATAGGTCATC TCCTGATAAC AGTCCCGTGC ACGGCTTAGT GCGGCAGGCA
    TCCGTCACCA CCGGATCAAA CATCCCCATC ATAACTGAAC TGGCTAAGCC TGGCAAACTA
    CTGCCTTTGG TTTCATTCAG TCAGGAAAAA AACAGTGGAA CCAACATACA AATGATAACA
    GAACTGGTGA ACGACTCCAA CGTTCAGTTT TTGGACCAAG ACGACGATGA CGACCCAGAC
    ACTGAGCTCT ACCTGACCCA GCCGTTCGCC TGTGGTACCG CATTCGCCGT CAGCGTGTTG
    GACTCCCTGA TGAGTGCGAC ATACTTCAAT GATAATATCC TCACCCTGAT CCGGACGCTG
    GTCACGGGAG GCGCCACGCC GGAGCTGGAG GCCCTGTTGG CTGAGGAGAA CGCTCTGAGA
    GGTGGCTACA GCACGCCGCA GACGCTGGCT AACAGGGACA GGTGTCGGGT CGCCCAGCTG
    GCCCTCTACG ATGGGCCCTT CGCAGACTTG GGGGACGGAG GTTGCTATGG AGACTTGTTC
    TGTAAAGCAT TAAAAACCTA CAACATGCTG TGCTTTGGAA TCTATAGATT GCGAGATGCA
    CATTTAGGTG CACCAAGCCA GTGCACAAAA AGATATGTCA TCACAAACCC GCCTTATGAG
    TTTGAGATGG TGCCCACGGA CCTGATCTTC TGTCTGATGC AGTTCGACCA CAACGCGGGC
    CAGTCCCGAG CGAGCCTGTC CCACTCCTCC CATTCGTCCC ATTCCTCCAG CAAGAAGAGC
    TCGTCTGTTC ACTCCATCCC AGCCACCAAC CGGCAGAACC GCAGCAGCAA AGCACGTGAA
    GCCCGTGACA AACAGAATGC AACAAGGATG AATAGAATGG GCCCAGAAAA GAGATGGTTT
    ACAGATGAAG CAGAAAACGC ATACCCAAGG AACATTCAAA TCAAGCCTAT GAGCACTCAC
    ATGGCCAACC AAGTCAATCA GTACAAATCC ACTAGCAGTC TGATTCCACC TATCCGAGAA
    GTCGAAGACG AATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305517.1
    CDS679..4596
    Translation

    Target ORF information:

    RefSeq Version XM_009307242.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307242.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT CCAAATATAA GTTTAACGGA
    TATGTCAGAT CACTCGAAGA TGAGCATCCG TCAACACTGT CACCCAAAAA GAAGCAGCGC
    AACGGAGGCA TGCGAAATTC ACCCAACTGC TCGCCCAAAA TGATGAGGCA TGATCCTCTA
    CTCATTCCAG GAAATGAGCA GATTGAGAGC ATGGATGCTA ATGTCAAACG ATACGACTCT
    ACTGGAATGT TTCACTGGTG TCCATCCAAA GAAATCGAGA AGGTCATTCT GACACGCAGC
    GAGGCCTCAA TGACGGTTTT AAGTGGACAT GTCGTAGTCT GCATATTTGG AGATGTCACG
    TCAGCTTTAG TGGGGCTTCG AAACTTAGTC ATGCCTTTGA GAGCAAGCAA CTTCCATTAC
    CATGAGCTAA AGCCCATTGT GTTTGTGGGC TCGTTAGATT ATCTGCGAAG AGAGTGGGAA
    ACCCTTCACA ACTTCCCCAA AGTCTTCATC TTACCTGGTA CGCCATTAAG TCGAGCTGAT
    TTAAGGGCCG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAC
    AATATCGACG ATGCTTCACT GCAGGACAAG GAATGCATCT TGGCATCTCT TAACATCAAA
    TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG TTTCACACCT
    CCAGGGATGG ATAGGTCATC TCCTGATAAC AGTCCCGTGC ACGGCTTAGT GCGGCAGGCA
    TCCGTCACCA CCGGATCAAA CATCCCCATC ATAACTGAAC TGGCTAAGCC TGGCAAACTA
    CTGCCTTTGG TTTCATTCAG TCAGGAAAAA AACAGTGGAA CCAACATACA AATGATAACA
    GAACTGGTGA ACGACTCCAA CGTTCAGTTT TTGGACCAAG ACGACGATGA CGACCCAGAC
    ACTGAGCTCT ACCTGACCCA GCCGTTCGCC TGTGGTACCG CATTCGCCGT CAGCGTGTTG
    GACTCCCTGA TGAGTGCGAC ATACTTCAAT GATAATATCC TCACCCTGAT CCGGACGCTG
    GTCACGGGAG GCGCCACGCC GGAGCTGGAG GCCCTGTTGG CTGAGGAGAA CGCTCTGAGA
    GGTGGCTACA GCACGCCGCA GACGCTGGCT AACAGGGACA GGTGTCGGGT CGCCCAGCTG
    GCCCTCTACG ATGGGCCCTT CGCAGACTTG GGGGACGGAG GTTGCTATGG AGACTTGTTC
    TGTAAAGCAT TAAAAACCTA CAACATGCTG TGCTTTGGAA TCTATAGATT GCGAGATGCA
    CATTTAGGTG CACCAAGCCA GTGCACAAAA AGATATGTCA TCACAAACCC GCCTTATGAG
    TTTGAGATGG TGCCCACGGA CCTGATCTTC TGTCTGATGC AGTTCGACCA CAACGCGGGC
    CAGTCCCGAG CGAGCCTGTC CCACTCCTCC CATTCGTCCC ATTCCTCCAG CAAGAAGAGC
    TCGTCTGTTC ACTCCATCCC AGCCACCAAC CGGCAGAACC GCAGCAGCAA AGCACGTGAA
    GCCCGTGACA AACAGAATGC AACAAGGATG AATAGAATGG GCCCAGAAAA GAGATGGTTT
    ACAGATGAAG CAGAAAACGC ATACCCAAGG AACATTCAAA TCAAGCCTAT GAGCACTCAC
    ATGGCCAACC AAGTCAATCA GTACAAATCC ACTAGCAGTC TGATTCCACC TATCCGAGAA
    GTCGAAGACG AATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45446
    Clone ID Related Accession (Same CDS sequence) XM_009307243.2 , XM_009307243.3
    Accession Version XM_009307243.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3909bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307243.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAACCA AGATGTCCGT CTACAAGCGA
    ATGAAACTGG CATGTTGTTT TGATTGCGGG CGCTCTGAGC GCGACTGCTC GTGCATGTCA
    GGCAGTGTAC ATAGTAACAT GGACAGCCTT CAGAGGGCCT TCCCACTTTC TTCTGTCTCT
    GTTCATGATT GCTCAACCAG TTTACGTGCC TCCAAATATA AGTTTAACGG ATATGTCAGA
    TCACTCGAAG ATGAGCATCC GTCAACACTG TCACCCAAAA AGAAGCAGCG CAACGGAGGC
    ATGCGAAATT CACCCAACTG CTCGCCCAAA ATGATGAGGC ATGATCCTCT ACTCATTCCA
    GGAAATGAGC AGATTGAGAG CATGGATGCT AATGTCAAAC GATACGACTC TACTGGAATG
    TTTCACTGGT GTCCATCCAA AGAAATCGAG AAGGTCATTC TGACACGCAG CGAGGCCTCA
    ATGACGGTTT TAAGTGGACA TGTCGTAGTC TGCATATTTG GAGATGTCAC GTCAGCTTTA
    GTGGGGCTTC GAAACTTAGT CATGCCTTTG AGAGCAAGCA ACTTCCATTA CCATGAGCTA
    AAGCCCATTG TGTTTGTGGG CTCGTTAGAT TATCTGCGAA GAGAGTGGGA AACCCTTCAC
    AACTTCCCCA AAGTCTTCAT CTTACCTGGT ACGCCATTAA GTCGAGCTGA TTTAAGGGCC
    GTCAACATCA ACCTCTGCGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA CAATATCGAC
    GATGCTTCAC TGCAGGACAA GGAATGCATC TTGGCATCTC TTAACATCAA ATCTATGCAG
    TTTGATGACA GCATTGGGGT CTTGCAGGCT AATTCCCAAG GTTTCACACC TCCAGGGATG
    GATAGGTCAT CTCCTGATAA CAGTCCCGTG CACGGCTTAG TGCGGCAGGC ATCCGTCACC
    ACCGGATCAA ACATCCCCAT CATAACTGAA CTGGCTAAGC CTGGCAAACT ACTGCCTTTG
    GTTTCATTCA GTCAGGAAAA AAACAGTGGA ACCAACATAC AAATGATAAC AGAACTGGTG
    AACGACTCCA ACGTTCAGTT TTTGGACCAA GACGACGATG ACGACCCAGA CACTGAGCTC
    TACCTGACCC AGCCGTTCGC CTGTGGTACC GCATTCGCCG TCAGCGTGTT GGACTCCCTG
    ATGAGTGCGA CATACTTCAA TGATAATATC CTCACCCTGA TCCGGACGCT GGTCACGGGA
    GGCGCCACGC CGGAGCTGGA GGCCCTGTTG GCTGAGGAGA ACGCTCTGAG AGGTGGCTAC
    AGCACGCCGC AGACGCTGGC TAACAGGGAC AGGTGTCGGG TCGCCCAGCT GGCCCTCTAC
    GATGGGCCCT TCGCAGACTT GGGGGACGGA GGTTGCTATG GAGACTTGTT CTGTAAAGCA
    TTAAAAACCT ACAACATGCT GTGCTTTGGA ATCTATAGAT TGCGAGATGC ACATTTAGGT
    GCACCAAGCC AGTGCACAAA AAGATATGTC ATCACAAACC CGCCTTATGA GTTTGAGATG
    GTGCCCACGG ACCTGATCTT CTGTCTGATG CAGTTCGACC ACAACGCGGG CCAGTCCCGA
    GCGAGCCTGT CCCACTCCTC CCATTCGTCC CATTCCTCCA GCAAGAAGAG CTCGTCTGTT
    CACTCCATCC CAGCCACCAA CCGGCAGAAC CGCAGCAGCA AAGCACGTGA AGCCCGTGAC
    AAACAGAATG CAACAAGGAT GAATAGAATG GGCCCAGAAA AGAGATGGTT TACAGATGAA
    GCAGAAAACG CATACCCAAG GAACATTCAA ATCAAGCCTA TGAGCACTCA CATGGCCAAC
    CAAGTCAATC AGTACAAATC CACTAGCAGT CTGATTCCAC CTATCCGAGA AGTCGAAGAC
    GAATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305518.1
    CDS661..4569
    Translation

    Target ORF information:

    RefSeq Version XM_009307243.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307243.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAACCA AGATGTCCGT CTACAAGCGA
    ATGAAACTGG CATGTTGTTT TGATTGCGGG CGCTCTGAGC GCGACTGCTC GTGCATGTCA
    GGCAGTGTAC ATAGTAACAT GGACAGCCTT CAGAGGGCCT TCCCACTTTC TTCTGTCTCT
    GTTCATGATT GCTCAACCAG TTTACGTGCC TCCAAATATA AGTTTAACGG ATATGTCAGA
    TCACTCGAAG ATGAGCATCC GTCAACACTG TCACCCAAAA AGAAGCAGCG CAACGGAGGC
    ATGCGAAATT CACCCAACTG CTCGCCCAAA ATGATGAGGC ATGATCCTCT ACTCATTCCA
    GGAAATGAGC AGATTGAGAG CATGGATGCT AATGTCAAAC GATACGACTC TACTGGAATG
    TTTCACTGGT GTCCATCCAA AGAAATCGAG AAGGTCATTC TGACACGCAG CGAGGCCTCA
    ATGACGGTTT TAAGTGGACA TGTCGTAGTC TGCATATTTG GAGATGTCAC GTCAGCTTTA
    GTGGGGCTTC GAAACTTAGT CATGCCTTTG AGAGCAAGCA ACTTCCATTA CCATGAGCTA
    AAGCCCATTG TGTTTGTGGG CTCGTTAGAT TATCTGCGAA GAGAGTGGGA AACCCTTCAC
    AACTTCCCCA AAGTCTTCAT CTTACCTGGT ACGCCATTAA GTCGAGCTGA TTTAAGGGCC
    GTCAACATCA ACCTCTGCGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA CAATATCGAC
    GATGCTTCAC TGCAGGACAA GGAATGCATC TTGGCATCTC TTAACATCAA ATCTATGCAG
    TTTGATGACA GCATTGGGGT CTTGCAGGCT AATTCCCAAG GTTTCACACC TCCAGGGATG
    GATAGGTCAT CTCCTGATAA CAGTCCCGTG CACGGCTTAG TGCGGCAGGC ATCCGTCACC
    ACCGGATCAA ACATCCCCAT CATAACTGAA CTGGCTAAGC CTGGCAAACT ACTGCCTTTG
    GTTTCATTCA GTCAGGAAAA AAACAGTGGA ACCAACATAC AAATGATAAC AGAACTGGTG
    AACGACTCCA ACGTTCAGTT TTTGGACCAA GACGACGATG ACGACCCAGA CACTGAGCTC
    TACCTGACCC AGCCGTTCGC CTGTGGTACC GCATTCGCCG TCAGCGTGTT GGACTCCCTG
    ATGAGTGCGA CATACTTCAA TGATAATATC CTCACCCTGA TCCGGACGCT GGTCACGGGA
    GGCGCCACGC CGGAGCTGGA GGCCCTGTTG GCTGAGGAGA ACGCTCTGAG AGGTGGCTAC
    AGCACGCCGC AGACGCTGGC TAACAGGGAC AGGTGTCGGG TCGCCCAGCT GGCCCTCTAC
    GATGGGCCCT TCGCAGACTT GGGGGACGGA GGTTGCTATG GAGACTTGTT CTGTAAAGCA
    TTAAAAACCT ACAACATGCT GTGCTTTGGA ATCTATAGAT TGCGAGATGC ACATTTAGGT
    GCACCAAGCC AGTGCACAAA AAGATATGTC ATCACAAACC CGCCTTATGA GTTTGAGATG
    GTGCCCACGG ACCTGATCTT CTGTCTGATG CAGTTCGACC ACAACGCGGG CCAGTCCCGA
    GCGAGCCTGT CCCACTCCTC CCATTCGTCC CATTCCTCCA GCAAGAAGAG CTCGTCTGTT
    CACTCCATCC CAGCCACCAA CCGGCAGAAC CGCAGCAGCA AAGCACGTGA AGCCCGTGAC
    AAACAGAATG CAACAAGGAT GAATAGAATG GGCCCAGAAA AGAGATGGTT TACAGATGAA
    GCAGAAAACG CATACCCAAG GAACATTCAA ATCAAGCCTA TGAGCACTCA CATGGCCAAC
    CAAGTCAATC AGTACAAATC CACTAGCAGT CTGATTCCAC CTATCCGAGA AGTCGAAGAC
    GAATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45446
    Clone ID Related Accession (Same CDS sequence) XM_009307243.2 , XM_009307243.3
    Accession Version XM_009307243.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3909bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307243.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAACCA AGATGTCCGT CTACAAGCGA
    ATGAAACTGG CATGTTGTTT TGATTGCGGG CGCTCTGAGC GCGACTGCTC GTGCATGTCA
    GGCAGTGTAC ATAGTAACAT GGACAGCCTT CAGAGGGCCT TCCCACTTTC TTCTGTCTCT
    GTTCATGATT GCTCAACCAG TTTACGTGCC TCCAAATATA AGTTTAACGG ATATGTCAGA
    TCACTCGAAG ATGAGCATCC GTCAACACTG TCACCCAAAA AGAAGCAGCG CAACGGAGGC
    ATGCGAAATT CACCCAACTG CTCGCCCAAA ATGATGAGGC ATGATCCTCT ACTCATTCCA
    GGAAATGAGC AGATTGAGAG CATGGATGCT AATGTCAAAC GATACGACTC TACTGGAATG
    TTTCACTGGT GTCCATCCAA AGAAATCGAG AAGGTCATTC TGACACGCAG CGAGGCCTCA
    ATGACGGTTT TAAGTGGACA TGTCGTAGTC TGCATATTTG GAGATGTCAC GTCAGCTTTA
    GTGGGGCTTC GAAACTTAGT CATGCCTTTG AGAGCAAGCA ACTTCCATTA CCATGAGCTA
    AAGCCCATTG TGTTTGTGGG CTCGTTAGAT TATCTGCGAA GAGAGTGGGA AACCCTTCAC
    AACTTCCCCA AAGTCTTCAT CTTACCTGGT ACGCCATTAA GTCGAGCTGA TTTAAGGGCC
    GTCAACATCA ACCTCTGCGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA CAATATCGAC
    GATGCTTCAC TGCAGGACAA GGAATGCATC TTGGCATCTC TTAACATCAA ATCTATGCAG
    TTTGATGACA GCATTGGGGT CTTGCAGGCT AATTCCCAAG GTTTCACACC TCCAGGGATG
    GATAGGTCAT CTCCTGATAA CAGTCCCGTG CACGGCTTAG TGCGGCAGGC ATCCGTCACC
    ACCGGATCAA ACATCCCCAT CATAACTGAA CTGGCTAAGC CTGGCAAACT ACTGCCTTTG
    GTTTCATTCA GTCAGGAAAA AAACAGTGGA ACCAACATAC AAATGATAAC AGAACTGGTG
    AACGACTCCA ACGTTCAGTT TTTGGACCAA GACGACGATG ACGACCCAGA CACTGAGCTC
    TACCTGACCC AGCCGTTCGC CTGTGGTACC GCATTCGCCG TCAGCGTGTT GGACTCCCTG
    ATGAGTGCGA CATACTTCAA TGATAATATC CTCACCCTGA TCCGGACGCT GGTCACGGGA
    GGCGCCACGC CGGAGCTGGA GGCCCTGTTG GCTGAGGAGA ACGCTCTGAG AGGTGGCTAC
    AGCACGCCGC AGACGCTGGC TAACAGGGAC AGGTGTCGGG TCGCCCAGCT GGCCCTCTAC
    GATGGGCCCT TCGCAGACTT GGGGGACGGA GGTTGCTATG GAGACTTGTT CTGTAAAGCA
    TTAAAAACCT ACAACATGCT GTGCTTTGGA ATCTATAGAT TGCGAGATGC ACATTTAGGT
    GCACCAAGCC AGTGCACAAA AAGATATGTC ATCACAAACC CGCCTTATGA GTTTGAGATG
    GTGCCCACGG ACCTGATCTT CTGTCTGATG CAGTTCGACC ACAACGCGGG CCAGTCCCGA
    GCGAGCCTGT CCCACTCCTC CCATTCGTCC CATTCCTCCA GCAAGAAGAG CTCGTCTGTT
    CACTCCATCC CAGCCACCAA CCGGCAGAAC CGCAGCAGCA AAGCACGTGA AGCCCGTGAC
    AAACAGAATG CAACAAGGAT GAATAGAATG GGCCCAGAAA AGAGATGGTT TACAGATGAA
    GCAGAAAACG CATACCCAAG GAACATTCAA ATCAAGCCTA TGAGCACTCA CATGGCCAAC
    CAAGTCAATC AGTACAAATC CACTAGCAGT CTGATTCCAC CTATCCGAGA AGTCGAAGAC
    GAATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305518.1
    CDS679..4587
    Translation

    Target ORF information:

    RefSeq Version XM_009307243.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307243.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAACCA AGATGTCCGT CTACAAGCGA
    ATGAAACTGG CATGTTGTTT TGATTGCGGG CGCTCTGAGC GCGACTGCTC GTGCATGTCA
    GGCAGTGTAC ATAGTAACAT GGACAGCCTT CAGAGGGCCT TCCCACTTTC TTCTGTCTCT
    GTTCATGATT GCTCAACCAG TTTACGTGCC TCCAAATATA AGTTTAACGG ATATGTCAGA
    TCACTCGAAG ATGAGCATCC GTCAACACTG TCACCCAAAA AGAAGCAGCG CAACGGAGGC
    ATGCGAAATT CACCCAACTG CTCGCCCAAA ATGATGAGGC ATGATCCTCT ACTCATTCCA
    GGAAATGAGC AGATTGAGAG CATGGATGCT AATGTCAAAC GATACGACTC TACTGGAATG
    TTTCACTGGT GTCCATCCAA AGAAATCGAG AAGGTCATTC TGACACGCAG CGAGGCCTCA
    ATGACGGTTT TAAGTGGACA TGTCGTAGTC TGCATATTTG GAGATGTCAC GTCAGCTTTA
    GTGGGGCTTC GAAACTTAGT CATGCCTTTG AGAGCAAGCA ACTTCCATTA CCATGAGCTA
    AAGCCCATTG TGTTTGTGGG CTCGTTAGAT TATCTGCGAA GAGAGTGGGA AACCCTTCAC
    AACTTCCCCA AAGTCTTCAT CTTACCTGGT ACGCCATTAA GTCGAGCTGA TTTAAGGGCC
    GTCAACATCA ACCTCTGCGA CATGTGCGTT ATCCTGTCAG CCAATCAGAA CAATATCGAC
    GATGCTTCAC TGCAGGACAA GGAATGCATC TTGGCATCTC TTAACATCAA ATCTATGCAG
    TTTGATGACA GCATTGGGGT CTTGCAGGCT AATTCCCAAG GTTTCACACC TCCAGGGATG
    GATAGGTCAT CTCCTGATAA CAGTCCCGTG CACGGCTTAG TGCGGCAGGC ATCCGTCACC
    ACCGGATCAA ACATCCCCAT CATAACTGAA CTGGCTAAGC CTGGCAAACT ACTGCCTTTG
    GTTTCATTCA GTCAGGAAAA AAACAGTGGA ACCAACATAC AAATGATAAC AGAACTGGTG
    AACGACTCCA ACGTTCAGTT TTTGGACCAA GACGACGATG ACGACCCAGA CACTGAGCTC
    TACCTGACCC AGCCGTTCGC CTGTGGTACC GCATTCGCCG TCAGCGTGTT GGACTCCCTG
    ATGAGTGCGA CATACTTCAA TGATAATATC CTCACCCTGA TCCGGACGCT GGTCACGGGA
    GGCGCCACGC CGGAGCTGGA GGCCCTGTTG GCTGAGGAGA ACGCTCTGAG AGGTGGCTAC
    AGCACGCCGC AGACGCTGGC TAACAGGGAC AGGTGTCGGG TCGCCCAGCT GGCCCTCTAC
    GATGGGCCCT TCGCAGACTT GGGGGACGGA GGTTGCTATG GAGACTTGTT CTGTAAAGCA
    TTAAAAACCT ACAACATGCT GTGCTTTGGA ATCTATAGAT TGCGAGATGC ACATTTAGGT
    GCACCAAGCC AGTGCACAAA AAGATATGTC ATCACAAACC CGCCTTATGA GTTTGAGATG
    GTGCCCACGG ACCTGATCTT CTGTCTGATG CAGTTCGACC ACAACGCGGG CCAGTCCCGA
    GCGAGCCTGT CCCACTCCTC CCATTCGTCC CATTCCTCCA GCAAGAAGAG CTCGTCTGTT
    CACTCCATCC CAGCCACCAA CCGGCAGAAC CGCAGCAGCA AAGCACGTGA AGCCCGTGAC
    AAACAGAATG CAACAAGGAT GAATAGAATG GGCCCAGAAA AGAGATGGTT TACAGATGAA
    GCAGAAAACG CATACCCAAG GAACATTCAA ATCAAGCCTA TGAGCACTCA CATGGCCAAC
    CAAGTCAATC AGTACAAATC CACTAGCAGT CTGATTCCAC CTATCCGAGA AGTCGAAGAC
    GAATGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45447
    Clone ID Related Accession (Same CDS sequence) XM_009307244.2 , XM_009307244.3
    Accession Version XM_009307244.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307244.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATGACA TATTCCAAGA TGTCCGTCTA CAAGCGAATG
    AAACTGGCAT GTTGTTTTGA TTGCGGGCGC TCTGAGCGCG ACTGCTCGTG CATGTCAGGC
    AGTGTACATA GTAACATGGA CAGCCTTCAG AGGGCCTTCC CACTTTCTTC TGTCTCTGTT
    CATGATTGCT CAACCAGTTT ACGTGCCTCC AAATATAAGT TTAACGGATA TGTCAGATCA
    CTCGAAGATG AGCATCCGTC AACACTGTCA CCCAAAAAGA AGCAGCGCAA CGGAGGCATG
    CGAAATTCAC CCAACTGCTC GCCCAAAATG ATGAGGCATG ATCCTCTACT CATTCCAGGA
    AATGAGCAGA TTGAGAGCAT GGATGCTAAT GTCAAACGAT ACGACTCTAC TGGAATGTTT
    CACTGGTGTC CATCCAAAGA AATCGAGAAG GTCATTCTGA CACGCAGCGA GGCCTCAATG
    ACGGTTTTAA GTGGACATGT CGTAGTCTGC ATATTTGGAG ATGTCACGTC AGCTTTAGTG
    GGGCTTCGAA ACTTAGTCAT GCCTTTGAGA GCAAGCAACT TCCATTACCA TGAGCTAAAG
    CCCATTGTGT TTGTGGGCTC GTTAGATTAT CTGCGAAGAG AGTGGGAAAC CCTTCACAAC
    TTCCCCAAAG TCTTCATCTT ACCTGGTACG CCATTAAGTC GAGCTGATTT AAGGGCCGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAACAA TATCGACGAT
    GCTTCACTGC AGGACAAGGA ATGCATCTTG GCATCTCTTA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGTT TCACACCTCC AGGGATGGAT
    AGGTCATCTC CTGATAACAG TCCCGTGCAC GGCTTAGTGC GGCAGGCATC CGTCACCACC
    GGATCAAACA TCCCCATCAT AACTGAACTG GCTAAGCCTG GCAAACTACT GCCTTTGGTT
    TCATTCAGTC AGGAAAAAAA CAGTGGAACC AACATACAAA TGATAACAGA ACTGGTGAAC
    GACTCCAACG TTCAGTTTTT GGACCAAGAC GACGATGACG ACCCAGACAC TGAGCTCTAC
    CTGACCCAGC CGTTCGCCTG TGGTACCGCA TTCGCCGTCA GCGTGTTGGA CTCCCTGATG
    AGTGCGACAT ACTTCAATGA TAATATCCTC ACCCTGATCC GGACGCTGGT CACGGGAGGC
    GCCACGCCGG AGCTGGAGGC CCTGTTGGCT GAGGAGAACG CTCTGAGAGG TGGCTACAGC
    ACGCCGCAGA CGCTGGCTAA CAGGGACAGG TGTCGGGTCG CCCAGCTGGC CCTCTACGAT
    GGGCCCTTCG CAGACTTGGG GGACGGAGGT TGCTATGGAG ACTTGTTCTG TAAAGCATTA
    AAAACCTACA ACATGCTGTG CTTTGGAATC TATAGATTGC GAGATGCACA TTTAGGTGCA
    CCAAGCCAGT GCACAAAAAG ATATGTCATC ACAAACCCGC CTTATGAGTT TGAGATGGTG
    CCCACGGACC TGATCTTCTG TCTGATGCAG TTCGACCACA ACGCGGGCCA GTCCCGAGCG
    AGCCTGTCCC ACTCCTCCCA TTCGTCCCAT TCCTCCAGCA AGAAGAGCTC GTCTGTTCAC
    TCCATCCCAG CCACCAACCG GCAGAACCGC AGCAGCAAAG CACGTGAAGC CCGTGACAAA
    CAGAATGCAA CAAGGATGAA TAGAATGGGC CCAGAAAAGA GATGGTTTAC AGATGAAGCA
    GAAAACGCAT ACCCAAGGAA CATTCAAATC AAGCCTATGA GCACTCACAT GGCCAACCAA
    GTCAATCAGT ACAAATCCAC TAGCAGTCTG ATTCCACCTA TCCGAGAAGT CGAAGACGAA
    TGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305519.1
    CDS659..4564
    Translation

    Target ORF information:

    RefSeq Version XM_009307244.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307244.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATGACA TATTCCAAGA TGTCCGTCTA CAAGCGAATG
    AAACTGGCAT GTTGTTTTGA TTGCGGGCGC TCTGAGCGCG ACTGCTCGTG CATGTCAGGC
    AGTGTACATA GTAACATGGA CAGCCTTCAG AGGGCCTTCC CACTTTCTTC TGTCTCTGTT
    CATGATTGCT CAACCAGTTT ACGTGCCTCC AAATATAAGT TTAACGGATA TGTCAGATCA
    CTCGAAGATG AGCATCCGTC AACACTGTCA CCCAAAAAGA AGCAGCGCAA CGGAGGCATG
    CGAAATTCAC CCAACTGCTC GCCCAAAATG ATGAGGCATG ATCCTCTACT CATTCCAGGA
    AATGAGCAGA TTGAGAGCAT GGATGCTAAT GTCAAACGAT ACGACTCTAC TGGAATGTTT
    CACTGGTGTC CATCCAAAGA AATCGAGAAG GTCATTCTGA CACGCAGCGA GGCCTCAATG
    ACGGTTTTAA GTGGACATGT CGTAGTCTGC ATATTTGGAG ATGTCACGTC AGCTTTAGTG
    GGGCTTCGAA ACTTAGTCAT GCCTTTGAGA GCAAGCAACT TCCATTACCA TGAGCTAAAG
    CCCATTGTGT TTGTGGGCTC GTTAGATTAT CTGCGAAGAG AGTGGGAAAC CCTTCACAAC
    TTCCCCAAAG TCTTCATCTT ACCTGGTACG CCATTAAGTC GAGCTGATTT AAGGGCCGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAACAA TATCGACGAT
    GCTTCACTGC AGGACAAGGA ATGCATCTTG GCATCTCTTA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGTT TCACACCTCC AGGGATGGAT
    AGGTCATCTC CTGATAACAG TCCCGTGCAC GGCTTAGTGC GGCAGGCATC CGTCACCACC
    GGATCAAACA TCCCCATCAT AACTGAACTG GCTAAGCCTG GCAAACTACT GCCTTTGGTT
    TCATTCAGTC AGGAAAAAAA CAGTGGAACC AACATACAAA TGATAACAGA ACTGGTGAAC
    GACTCCAACG TTCAGTTTTT GGACCAAGAC GACGATGACG ACCCAGACAC TGAGCTCTAC
    CTGACCCAGC CGTTCGCCTG TGGTACCGCA TTCGCCGTCA GCGTGTTGGA CTCCCTGATG
    AGTGCGACAT ACTTCAATGA TAATATCCTC ACCCTGATCC GGACGCTGGT CACGGGAGGC
    GCCACGCCGG AGCTGGAGGC CCTGTTGGCT GAGGAGAACG CTCTGAGAGG TGGCTACAGC
    ACGCCGCAGA CGCTGGCTAA CAGGGACAGG TGTCGGGTCG CCCAGCTGGC CCTCTACGAT
    GGGCCCTTCG CAGACTTGGG GGACGGAGGT TGCTATGGAG ACTTGTTCTG TAAAGCATTA
    AAAACCTACA ACATGCTGTG CTTTGGAATC TATAGATTGC GAGATGCACA TTTAGGTGCA
    CCAAGCCAGT GCACAAAAAG ATATGTCATC ACAAACCCGC CTTATGAGTT TGAGATGGTG
    CCCACGGACC TGATCTTCTG TCTGATGCAG TTCGACCACA ACGCGGGCCA GTCCCGAGCG
    AGCCTGTCCC ACTCCTCCCA TTCGTCCCAT TCCTCCAGCA AGAAGAGCTC GTCTGTTCAC
    TCCATCCCAG CCACCAACCG GCAGAACCGC AGCAGCAAAG CACGTGAAGC CCGTGACAAA
    CAGAATGCAA CAAGGATGAA TAGAATGGGC CCAGAAAAGA GATGGTTTAC AGATGAAGCA
    GAAAACGCAT ACCCAAGGAA CATTCAAATC AAGCCTATGA GCACTCACAT GGCCAACCAA
    GTCAATCAGT ACAAATCCAC TAGCAGTCTG ATTCCACCTA TCCGAGAAGT CGAAGACGAA
    TGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45447
    Clone ID Related Accession (Same CDS sequence) XM_009307244.2 , XM_009307244.3
    Accession Version XM_009307244.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3906bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307244.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATGACA TATTCCAAGA TGTCCGTCTA CAAGCGAATG
    AAACTGGCAT GTTGTTTTGA TTGCGGGCGC TCTGAGCGCG ACTGCTCGTG CATGTCAGGC
    AGTGTACATA GTAACATGGA CAGCCTTCAG AGGGCCTTCC CACTTTCTTC TGTCTCTGTT
    CATGATTGCT CAACCAGTTT ACGTGCCTCC AAATATAAGT TTAACGGATA TGTCAGATCA
    CTCGAAGATG AGCATCCGTC AACACTGTCA CCCAAAAAGA AGCAGCGCAA CGGAGGCATG
    CGAAATTCAC CCAACTGCTC GCCCAAAATG ATGAGGCATG ATCCTCTACT CATTCCAGGA
    AATGAGCAGA TTGAGAGCAT GGATGCTAAT GTCAAACGAT ACGACTCTAC TGGAATGTTT
    CACTGGTGTC CATCCAAAGA AATCGAGAAG GTCATTCTGA CACGCAGCGA GGCCTCAATG
    ACGGTTTTAA GTGGACATGT CGTAGTCTGC ATATTTGGAG ATGTCACGTC AGCTTTAGTG
    GGGCTTCGAA ACTTAGTCAT GCCTTTGAGA GCAAGCAACT TCCATTACCA TGAGCTAAAG
    CCCATTGTGT TTGTGGGCTC GTTAGATTAT CTGCGAAGAG AGTGGGAAAC CCTTCACAAC
    TTCCCCAAAG TCTTCATCTT ACCTGGTACG CCATTAAGTC GAGCTGATTT AAGGGCCGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAACAA TATCGACGAT
    GCTTCACTGC AGGACAAGGA ATGCATCTTG GCATCTCTTA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGTT TCACACCTCC AGGGATGGAT
    AGGTCATCTC CTGATAACAG TCCCGTGCAC GGCTTAGTGC GGCAGGCATC CGTCACCACC
    GGATCAAACA TCCCCATCAT AACTGAACTG GCTAAGCCTG GCAAACTACT GCCTTTGGTT
    TCATTCAGTC AGGAAAAAAA CAGTGGAACC AACATACAAA TGATAACAGA ACTGGTGAAC
    GACTCCAACG TTCAGTTTTT GGACCAAGAC GACGATGACG ACCCAGACAC TGAGCTCTAC
    CTGACCCAGC CGTTCGCCTG TGGTACCGCA TTCGCCGTCA GCGTGTTGGA CTCCCTGATG
    AGTGCGACAT ACTTCAATGA TAATATCCTC ACCCTGATCC GGACGCTGGT CACGGGAGGC
    GCCACGCCGG AGCTGGAGGC CCTGTTGGCT GAGGAGAACG CTCTGAGAGG TGGCTACAGC
    ACGCCGCAGA CGCTGGCTAA CAGGGACAGG TGTCGGGTCG CCCAGCTGGC CCTCTACGAT
    GGGCCCTTCG CAGACTTGGG GGACGGAGGT TGCTATGGAG ACTTGTTCTG TAAAGCATTA
    AAAACCTACA ACATGCTGTG CTTTGGAATC TATAGATTGC GAGATGCACA TTTAGGTGCA
    CCAAGCCAGT GCACAAAAAG ATATGTCATC ACAAACCCGC CTTATGAGTT TGAGATGGTG
    CCCACGGACC TGATCTTCTG TCTGATGCAG TTCGACCACA ACGCGGGCCA GTCCCGAGCG
    AGCCTGTCCC ACTCCTCCCA TTCGTCCCAT TCCTCCAGCA AGAAGAGCTC GTCTGTTCAC
    TCCATCCCAG CCACCAACCG GCAGAACCGC AGCAGCAAAG CACGTGAAGC CCGTGACAAA
    CAGAATGCAA CAAGGATGAA TAGAATGGGC CCAGAAAAGA GATGGTTTAC AGATGAAGCA
    GAAAACGCAT ACCCAAGGAA CATTCAAATC AAGCCTATGA GCACTCACAT GGCCAACCAA
    GTCAATCAGT ACAAATCCAC TAGCAGTCTG ATTCCACCTA TCCGAGAAGT CGAAGACGAA
    TGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305519.1
    CDS677..4582
    Translation

    Target ORF information:

    RefSeq Version XM_009307244.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307244.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GTATTCTGAT CAACCCGGGA
    AATCACGTTA AAATGCAGGA GGGGACACTG GGATTCTTTA TTGCCAGTGA TGCCAAAGAA
    GTGAAAAGAG CATTTTTCTA CTGCAAAGCA TGTCATGATG ACATCACAGA TCCCAAACGG
    ATTAAAAAGT GTGGCTGCAA GAGAATGACA TATTCCAAGA TGTCCGTCTA CAAGCGAATG
    AAACTGGCAT GTTGTTTTGA TTGCGGGCGC TCTGAGCGCG ACTGCTCGTG CATGTCAGGC
    AGTGTACATA GTAACATGGA CAGCCTTCAG AGGGCCTTCC CACTTTCTTC TGTCTCTGTT
    CATGATTGCT CAACCAGTTT ACGTGCCTCC AAATATAAGT TTAACGGATA TGTCAGATCA
    CTCGAAGATG AGCATCCGTC AACACTGTCA CCCAAAAAGA AGCAGCGCAA CGGAGGCATG
    CGAAATTCAC CCAACTGCTC GCCCAAAATG ATGAGGCATG ATCCTCTACT CATTCCAGGA
    AATGAGCAGA TTGAGAGCAT GGATGCTAAT GTCAAACGAT ACGACTCTAC TGGAATGTTT
    CACTGGTGTC CATCCAAAGA AATCGAGAAG GTCATTCTGA CACGCAGCGA GGCCTCAATG
    ACGGTTTTAA GTGGACATGT CGTAGTCTGC ATATTTGGAG ATGTCACGTC AGCTTTAGTG
    GGGCTTCGAA ACTTAGTCAT GCCTTTGAGA GCAAGCAACT TCCATTACCA TGAGCTAAAG
    CCCATTGTGT TTGTGGGCTC GTTAGATTAT CTGCGAAGAG AGTGGGAAAC CCTTCACAAC
    TTCCCCAAAG TCTTCATCTT ACCTGGTACG CCATTAAGTC GAGCTGATTT AAGGGCCGTC
    AACATCAACC TCTGCGACAT GTGCGTTATC CTGTCAGCCA ATCAGAACAA TATCGACGAT
    GCTTCACTGC AGGACAAGGA ATGCATCTTG GCATCTCTTA ACATCAAATC TATGCAGTTT
    GATGACAGCA TTGGGGTCTT GCAGGCTAAT TCCCAAGGTT TCACACCTCC AGGGATGGAT
    AGGTCATCTC CTGATAACAG TCCCGTGCAC GGCTTAGTGC GGCAGGCATC CGTCACCACC
    GGATCAAACA TCCCCATCAT AACTGAACTG GCTAAGCCTG GCAAACTACT GCCTTTGGTT
    TCATTCAGTC AGGAAAAAAA CAGTGGAACC AACATACAAA TGATAACAGA ACTGGTGAAC
    GACTCCAACG TTCAGTTTTT GGACCAAGAC GACGATGACG ACCCAGACAC TGAGCTCTAC
    CTGACCCAGC CGTTCGCCTG TGGTACCGCA TTCGCCGTCA GCGTGTTGGA CTCCCTGATG
    AGTGCGACAT ACTTCAATGA TAATATCCTC ACCCTGATCC GGACGCTGGT CACGGGAGGC
    GCCACGCCGG AGCTGGAGGC CCTGTTGGCT GAGGAGAACG CTCTGAGAGG TGGCTACAGC
    ACGCCGCAGA CGCTGGCTAA CAGGGACAGG TGTCGGGTCG CCCAGCTGGC CCTCTACGAT
    GGGCCCTTCG CAGACTTGGG GGACGGAGGT TGCTATGGAG ACTTGTTCTG TAAAGCATTA
    AAAACCTACA ACATGCTGTG CTTTGGAATC TATAGATTGC GAGATGCACA TTTAGGTGCA
    CCAAGCCAGT GCACAAAAAG ATATGTCATC ACAAACCCGC CTTATGAGTT TGAGATGGTG
    CCCACGGACC TGATCTTCTG TCTGATGCAG TTCGACCACA ACGCGGGCCA GTCCCGAGCG
    AGCCTGTCCC ACTCCTCCCA TTCGTCCCAT TCCTCCAGCA AGAAGAGCTC GTCTGTTCAC
    TCCATCCCAG CCACCAACCG GCAGAACCGC AGCAGCAAAG CACGTGAAGC CCGTGACAAA
    CAGAATGCAA CAAGGATGAA TAGAATGGGC CCAGAAAAGA GATGGTTTAC AGATGAAGCA
    GAAAACGCAT ACCCAAGGAA CATTCAAATC AAGCCTATGA GCACTCACAT GGCCAACCAA
    GTCAATCAGT ACAAATCCAC TAGCAGTCTG ATTCCACCTA TCCGAGAAGT CGAAGACGAA
    TGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45448
    Clone ID Related Accession (Same CDS sequence) XM_009307245.2 , XM_009307245.3
    Accession Version XM_009307245.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3834bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307245.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT CCAAATATAA GTTTAACGGA
    TATGTCAGAT CACTCGAAGA TGAGCATCCG TCAACACTGT CACCCAAAAA GAAGCAGCGC
    AACGGAGGCA TGCGAAATTC ACCCAACTGC TCGCCCAAAA TGATGAGGCA TGATCCTCTA
    CTCATTCCAG GAAATGAGCA GATTGAGAGC ATGGATGCTA ATGTCAAACG ATACGACTCT
    ACTGGAATGT TTCACTGGTG TCCATCCAAA GAAATCGAGA AGGTCATTCT GACACGCAGC
    GAGGCCTCAA TGACGGTTTT AAGTGGACAT GTCGTAGTCT GCATATTTGG AGATGTCACG
    TCAGCTTTAG TGGGGCTTCG AAACTTAGTC ATGCCTTTGA GAGCAAGCAA CTTCCATTAC
    CATGAGCTAA AGCCCATTGT GTTTGTGGGC TCGTTAGATT ATCTGCGAAG AGAGTGGGAA
    ACCCTTCACA ACTTCCCCAA AGTCTTCATC TTACCTGGTA CGCCATTAAG TCGAGCTGAT
    TTAAGGGCCG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAC
    AATATCGACG ATGCTTCACT GCAGGACAAG GAATGCATCT TGGCATCTCT TAACATCAAA
    TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG TTTCACACCT
    CCAGGGATGG ATAGGTCATC TCCTGATAAC AGTCCCGTGC ACGGCTTAGT GCGGCAGGCA
    TCCGTCACCA CCGGATCAAA CATCCCCATC ATAACTGAAC TGGTGAACGA CTCCAACGTT
    CAGTTTTTGG ACCAAGACGA CGATGACGAC CCAGACACTG AGCTCTACCT GACCCAGCCG
    TTCGCCTGTG GTACCGCATT CGCCGTCAGC GTGTTGGACT CCCTGATGAG TGCGACATAC
    TTCAATGATA ATATCCTCAC CCTGATCCGG ACGCTGGTCA CGGGAGGCGC CACGCCGGAG
    CTGGAGGCCC TGTTGGCTGA GGAGAACGCT CTGAGAGGTG GCTACAGCAC GCCGCAGACG
    CTGGCTAACA GGGACAGGTG TCGGGTCGCC CAGCTGGCCC TCTACGATGG GCCCTTCGCA
    GACTTGGGGG ACGGAGGTTG CTATGGAGAC TTGTTCTGTA AAGCATTAAA AACCTACAAC
    ATGCTGTGCT TTGGAATCTA TAGATTGCGA GATGCACATT TAGGTGCACC AAGCCAGTGC
    ACAAAAAGAT ATGTCATCAC AAACCCGCCT TATGAGTTTG AGATGGTGCC CACGGACCTG
    ATCTTCTGTC TGATGCAGTT CGACCACAAC GCGGGCCAGT CCCGAGCGAG CCTGTCCCAC
    TCCTCCCATT CGTCCCATTC CTCCAGCAAG AAGAGCTCGT CTGTTCACTC CATCCCAGCC
    ACCAACCGGC AGAACCGCAG CAGCAAAGCA CGTGAAGCCC GTGACAAACA GAATGCAACA
    AGGATGAATA GAATGGGCCC AGAAAAGAGA TGGTTTACAG ATGAAGCAGA AAACGCATAC
    CCAAGGAACA TTCAAATCAA GCCTATGAGC ACTCACATGG CCAACCAAGT CAATCAGTAC
    AAATCCACTA GCAGTCTGAT TCCACCTATC CGAGAAGTCG AAGACGAATG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305520.1
    CDS658..4491
    Translation

    Target ORF information:

    RefSeq Version XM_009307245.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307245.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT CCAAATATAA GTTTAACGGA
    TATGTCAGAT CACTCGAAGA TGAGCATCCG TCAACACTGT CACCCAAAAA GAAGCAGCGC
    AACGGAGGCA TGCGAAATTC ACCCAACTGC TCGCCCAAAA TGATGAGGCA TGATCCTCTA
    CTCATTCCAG GAAATGAGCA GATTGAGAGC ATGGATGCTA ATGTCAAACG ATACGACTCT
    ACTGGAATGT TTCACTGGTG TCCATCCAAA GAAATCGAGA AGGTCATTCT GACACGCAGC
    GAGGCCTCAA TGACGGTTTT AAGTGGACAT GTCGTAGTCT GCATATTTGG AGATGTCACG
    TCAGCTTTAG TGGGGCTTCG AAACTTAGTC ATGCCTTTGA GAGCAAGCAA CTTCCATTAC
    CATGAGCTAA AGCCCATTGT GTTTGTGGGC TCGTTAGATT ATCTGCGAAG AGAGTGGGAA
    ACCCTTCACA ACTTCCCCAA AGTCTTCATC TTACCTGGTA CGCCATTAAG TCGAGCTGAT
    TTAAGGGCCG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAC
    AATATCGACG ATGCTTCACT GCAGGACAAG GAATGCATCT TGGCATCTCT TAACATCAAA
    TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG TTTCACACCT
    CCAGGGATGG ATAGGTCATC TCCTGATAAC AGTCCCGTGC ACGGCTTAGT GCGGCAGGCA
    TCCGTCACCA CCGGATCAAA CATCCCCATC ATAACTGAAC TGGTGAACGA CTCCAACGTT
    CAGTTTTTGG ACCAAGACGA CGATGACGAC CCAGACACTG AGCTCTACCT GACCCAGCCG
    TTCGCCTGTG GTACCGCATT CGCCGTCAGC GTGTTGGACT CCCTGATGAG TGCGACATAC
    TTCAATGATA ATATCCTCAC CCTGATCCGG ACGCTGGTCA CGGGAGGCGC CACGCCGGAG
    CTGGAGGCCC TGTTGGCTGA GGAGAACGCT CTGAGAGGTG GCTACAGCAC GCCGCAGACG
    CTGGCTAACA GGGACAGGTG TCGGGTCGCC CAGCTGGCCC TCTACGATGG GCCCTTCGCA
    GACTTGGGGG ACGGAGGTTG CTATGGAGAC TTGTTCTGTA AAGCATTAAA AACCTACAAC
    ATGCTGTGCT TTGGAATCTA TAGATTGCGA GATGCACATT TAGGTGCACC AAGCCAGTGC
    ACAAAAAGAT ATGTCATCAC AAACCCGCCT TATGAGTTTG AGATGGTGCC CACGGACCTG
    ATCTTCTGTC TGATGCAGTT CGACCACAAC GCGGGCCAGT CCCGAGCGAG CCTGTCCCAC
    TCCTCCCATT CGTCCCATTC CTCCAGCAAG AAGAGCTCGT CTGTTCACTC CATCCCAGCC
    ACCAACCGGC AGAACCGCAG CAGCAAAGCA CGTGAAGCCC GTGACAAACA GAATGCAACA
    AGGATGAATA GAATGGGCCC AGAAAAGAGA TGGTTTACAG ATGAAGCAGA AAACGCATAC
    CCAAGGAACA TTCAAATCAA GCCTATGAGC ACTCACATGG CCAACCAAGT CAATCAGTAC
    AAATCCACTA GCAGTCTGAT TCCACCTATC CGAGAAGTCG AAGACGAATG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45448
    Clone ID Related Accession (Same CDS sequence) XM_009307245.2 , XM_009307245.3
    Accession Version XM_009307245.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3834bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307245.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT CCAAATATAA GTTTAACGGA
    TATGTCAGAT CACTCGAAGA TGAGCATCCG TCAACACTGT CACCCAAAAA GAAGCAGCGC
    AACGGAGGCA TGCGAAATTC ACCCAACTGC TCGCCCAAAA TGATGAGGCA TGATCCTCTA
    CTCATTCCAG GAAATGAGCA GATTGAGAGC ATGGATGCTA ATGTCAAACG ATACGACTCT
    ACTGGAATGT TTCACTGGTG TCCATCCAAA GAAATCGAGA AGGTCATTCT GACACGCAGC
    GAGGCCTCAA TGACGGTTTT AAGTGGACAT GTCGTAGTCT GCATATTTGG AGATGTCACG
    TCAGCTTTAG TGGGGCTTCG AAACTTAGTC ATGCCTTTGA GAGCAAGCAA CTTCCATTAC
    CATGAGCTAA AGCCCATTGT GTTTGTGGGC TCGTTAGATT ATCTGCGAAG AGAGTGGGAA
    ACCCTTCACA ACTTCCCCAA AGTCTTCATC TTACCTGGTA CGCCATTAAG TCGAGCTGAT
    TTAAGGGCCG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAC
    AATATCGACG ATGCTTCACT GCAGGACAAG GAATGCATCT TGGCATCTCT TAACATCAAA
    TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG TTTCACACCT
    CCAGGGATGG ATAGGTCATC TCCTGATAAC AGTCCCGTGC ACGGCTTAGT GCGGCAGGCA
    TCCGTCACCA CCGGATCAAA CATCCCCATC ATAACTGAAC TGGTGAACGA CTCCAACGTT
    CAGTTTTTGG ACCAAGACGA CGATGACGAC CCAGACACTG AGCTCTACCT GACCCAGCCG
    TTCGCCTGTG GTACCGCATT CGCCGTCAGC GTGTTGGACT CCCTGATGAG TGCGACATAC
    TTCAATGATA ATATCCTCAC CCTGATCCGG ACGCTGGTCA CGGGAGGCGC CACGCCGGAG
    CTGGAGGCCC TGTTGGCTGA GGAGAACGCT CTGAGAGGTG GCTACAGCAC GCCGCAGACG
    CTGGCTAACA GGGACAGGTG TCGGGTCGCC CAGCTGGCCC TCTACGATGG GCCCTTCGCA
    GACTTGGGGG ACGGAGGTTG CTATGGAGAC TTGTTCTGTA AAGCATTAAA AACCTACAAC
    ATGCTGTGCT TTGGAATCTA TAGATTGCGA GATGCACATT TAGGTGCACC AAGCCAGTGC
    ACAAAAAGAT ATGTCATCAC AAACCCGCCT TATGAGTTTG AGATGGTGCC CACGGACCTG
    ATCTTCTGTC TGATGCAGTT CGACCACAAC GCGGGCCAGT CCCGAGCGAG CCTGTCCCAC
    TCCTCCCATT CGTCCCATTC CTCCAGCAAG AAGAGCTCGT CTGTTCACTC CATCCCAGCC
    ACCAACCGGC AGAACCGCAG CAGCAAAGCA CGTGAAGCCC GTGACAAACA GAATGCAACA
    AGGATGAATA GAATGGGCCC AGAAAAGAGA TGGTTTACAG ATGAAGCAGA AAACGCATAC
    CCAAGGAACA TTCAAATCAA GCCTATGAGC ACTCACATGG CCAACCAAGT CAATCAGTAC
    AAATCCACTA GCAGTCTGAT TCCACCTATC CGAGAAGTCG AAGACGAATG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305520.1
    CDS677..4510
    Translation

    Target ORF information:

    RefSeq Version XM_009307245.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307245.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT CCAAATATAA GTTTAACGGA
    TATGTCAGAT CACTCGAAGA TGAGCATCCG TCAACACTGT CACCCAAAAA GAAGCAGCGC
    AACGGAGGCA TGCGAAATTC ACCCAACTGC TCGCCCAAAA TGATGAGGCA TGATCCTCTA
    CTCATTCCAG GAAATGAGCA GATTGAGAGC ATGGATGCTA ATGTCAAACG ATACGACTCT
    ACTGGAATGT TTCACTGGTG TCCATCCAAA GAAATCGAGA AGGTCATTCT GACACGCAGC
    GAGGCCTCAA TGACGGTTTT AAGTGGACAT GTCGTAGTCT GCATATTTGG AGATGTCACG
    TCAGCTTTAG TGGGGCTTCG AAACTTAGTC ATGCCTTTGA GAGCAAGCAA CTTCCATTAC
    CATGAGCTAA AGCCCATTGT GTTTGTGGGC TCGTTAGATT ATCTGCGAAG AGAGTGGGAA
    ACCCTTCACA ACTTCCCCAA AGTCTTCATC TTACCTGGTA CGCCATTAAG TCGAGCTGAT
    TTAAGGGCCG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAC
    AATATCGACG ATGCTTCACT GCAGGACAAG GAATGCATCT TGGCATCTCT TAACATCAAA
    TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG TTTCACACCT
    CCAGGGATGG ATAGGTCATC TCCTGATAAC AGTCCCGTGC ACGGCTTAGT GCGGCAGGCA
    TCCGTCACCA CCGGATCAAA CATCCCCATC ATAACTGAAC TGGTGAACGA CTCCAACGTT
    CAGTTTTTGG ACCAAGACGA CGATGACGAC CCAGACACTG AGCTCTACCT GACCCAGCCG
    TTCGCCTGTG GTACCGCATT CGCCGTCAGC GTGTTGGACT CCCTGATGAG TGCGACATAC
    TTCAATGATA ATATCCTCAC CCTGATCCGG ACGCTGGTCA CGGGAGGCGC CACGCCGGAG
    CTGGAGGCCC TGTTGGCTGA GGAGAACGCT CTGAGAGGTG GCTACAGCAC GCCGCAGACG
    CTGGCTAACA GGGACAGGTG TCGGGTCGCC CAGCTGGCCC TCTACGATGG GCCCTTCGCA
    GACTTGGGGG ACGGAGGTTG CTATGGAGAC TTGTTCTGTA AAGCATTAAA AACCTACAAC
    ATGCTGTGCT TTGGAATCTA TAGATTGCGA GATGCACATT TAGGTGCACC AAGCCAGTGC
    ACAAAAAGAT ATGTCATCAC AAACCCGCCT TATGAGTTTG AGATGGTGCC CACGGACCTG
    ATCTTCTGTC TGATGCAGTT CGACCACAAC GCGGGCCAGT CCCGAGCGAG CCTGTCCCAC
    TCCTCCCATT CGTCCCATTC CTCCAGCAAG AAGAGCTCGT CTGTTCACTC CATCCCAGCC
    ACCAACCGGC AGAACCGCAG CAGCAAAGCA CGTGAAGCCC GTGACAAACA GAATGCAACA
    AGGATGAATA GAATGGGCCC AGAAAAGAGA TGGTTTACAG ATGAAGCAGA AAACGCATAC
    CCAAGGAACA TTCAAATCAA GCCTATGAGC ACTCACATGG CCAACCAAGT CAATCAGTAC
    AAATCCACTA GCAGTCTGAT TCCACCTATC CGAGAAGTCG AAGACGAATG CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45449
    Clone ID Related Accession (Same CDS sequence) XM_009307246.2 , XM_009307246.3
    Accession Version XM_009307246.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3792bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307246.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAACCA AGATGTCCGT CTACAAGCGA
    ATGAAACTGG CATGTTGTTT TGATTGCGGG CGCTCTGAGC GCGACTGCTC GTGCATGTCA
    GGCAGTGTAC ATAGTAACAT GGACAGCCTT CAGAGGGCCT TCCCACTTTC TTCTGTCTCT
    GTTCATGATT GCTCAACCAG TTTACGTGCC TTCGAAGATG AGCATCCGTC AACACTGTCA
    CCCAAAAAGA AGCAGCGCAA CGGAGGCATG CGAAATTCAC CCAACTGCTC GCCCAAAATG
    ATGAGGCATG ATCCTCTACT CATTCCAGGA AATGAGCAGA TTGAGAGCAT GGATGCTAAT
    GTCAAACGAT ACGACTCTAC TGGAATGTTT CACTGGTGTC CATCCAAAGA AATCGAGAAG
    GTCATTCTGA CACGCAGCGA GGCCTCAATG ACGGTTTTAA GTGGACATGT CGTAGTCTGC
    ATATTTGGAG ATGTCACGTC AGCTTTAGTG GGGCTTCGAA ACTTAGTCAT GCCTTTGAGA
    GCAAGCAACT TCCATTACCA TGAGCTAAAG CCCATTGTGT TTGTGGGCTC GTTAGATTAT
    CTGCGAAGAG AGTGGGAAAC CCTTCACAAC TTCCCCAAAG TCTTCATCTT ACCTGGTACG
    CCATTAAGTC GAGCTGATTT AAGGGCCGTC AACATCAACC TCTGCGACAT GTGCGTTATC
    CTGTCAGCCA ATCAGAACAA TATCGACGAT GCTTCACTGC AGGACAAGGA ATGCATCTTG
    GCATCTCTTA ACATCAAATC TATGCAGTTT GATGACAGCA TTGGGGTCTT GCAGGCTAAT
    TCCCAAGGTT TCACACCTCC AGGGATGGAT AGGTCATCTC CTGATAACAG TCCCGTGCAC
    GGCTTAGTGC GGCAGGCATC CGTCACCACC GGATCAAACA TCCCCATCAT AACTGAACTG
    GTGAACGACT CCAACGTTCA GTTTTTGGAC CAAGACGACG ATGACGACCC AGACACTGAG
    CTCTACCTGA CCCAGCCGTT CGCCTGTGGT ACCGCATTCG CCGTCAGCGT GTTGGACTCC
    CTGATGAGTG CGACATACTT CAATGATAAT ATCCTCACCC TGATCCGGAC GCTGGTCACG
    GGAGGCGCCA CGCCGGAGCT GGAGGCCCTG TTGGCTGAGG AGAACGCTCT GAGAGGTGGC
    TACAGCACGC CGCAGACGCT GGCTAACAGG GACAGGTGTC GGGTCGCCCA GCTGGCCCTC
    TACGATGGGC CCTTCGCAGA CTTGGGGGAC GGAGGTTGCT ATGGAGACTT GTTCTGTAAA
    GCATTAAAAA CCTACAACAT GCTGTGCTTT GGAATCTATA GATTGCGAGA TGCACATTTA
    GGTGCACCAA GCCAGTGCAC AAAAAGATAT GTCATCACAA ACCCGCCTTA TGAGTTTGAG
    ATGGTGCCCA CGGACCTGAT CTTCTGTCTG ATGCAGTTCG ACCACAACGC GGGCCAGTCC
    CGAGCGAGCC TGTCCCACTC CTCCCATTCG TCCCATTCCT CCAGCAAGAA GAGCTCGTCT
    GTTCACTCCA TCCCAGCCAC CAACCGGCAG AACCGCAGCA GCAAAGCACG TGAAGCCCGT
    GACAAACAGA ATGCAACAAG GATGAATAGA ATGGGCCCAG AAAAGAGATG GTTTACAGAT
    GAAGCAGAAA ACGCATACCC AAGGAACATT CAAATCAAGC CTATGAGCAC TCACATGGCC
    AACCAAGTCA ATCAGTACAA ATCCACTAGC AGTCTGATTC CACCTATCCG AGAAGTCGAA
    GACGAATGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305521.1
    CDS654..4445
    Translation

    Target ORF information:

    RefSeq Version XM_009307246.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307246.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAACCA AGATGTCCGT CTACAAGCGA
    ATGAAACTGG CATGTTGTTT TGATTGCGGG CGCTCTGAGC GCGACTGCTC GTGCATGTCA
    GGCAGTGTAC ATAGTAACAT GGACAGCCTT CAGAGGGCCT TCCCACTTTC TTCTGTCTCT
    GTTCATGATT GCTCAACCAG TTTACGTGCC TTCGAAGATG AGCATCCGTC AACACTGTCA
    CCCAAAAAGA AGCAGCGCAA CGGAGGCATG CGAAATTCAC CCAACTGCTC GCCCAAAATG
    ATGAGGCATG ATCCTCTACT CATTCCAGGA AATGAGCAGA TTGAGAGCAT GGATGCTAAT
    GTCAAACGAT ACGACTCTAC TGGAATGTTT CACTGGTGTC CATCCAAAGA AATCGAGAAG
    GTCATTCTGA CACGCAGCGA GGCCTCAATG ACGGTTTTAA GTGGACATGT CGTAGTCTGC
    ATATTTGGAG ATGTCACGTC AGCTTTAGTG GGGCTTCGAA ACTTAGTCAT GCCTTTGAGA
    GCAAGCAACT TCCATTACCA TGAGCTAAAG CCCATTGTGT TTGTGGGCTC GTTAGATTAT
    CTGCGAAGAG AGTGGGAAAC CCTTCACAAC TTCCCCAAAG TCTTCATCTT ACCTGGTACG
    CCATTAAGTC GAGCTGATTT AAGGGCCGTC AACATCAACC TCTGCGACAT GTGCGTTATC
    CTGTCAGCCA ATCAGAACAA TATCGACGAT GCTTCACTGC AGGACAAGGA ATGCATCTTG
    GCATCTCTTA ACATCAAATC TATGCAGTTT GATGACAGCA TTGGGGTCTT GCAGGCTAAT
    TCCCAAGGTT TCACACCTCC AGGGATGGAT AGGTCATCTC CTGATAACAG TCCCGTGCAC
    GGCTTAGTGC GGCAGGCATC CGTCACCACC GGATCAAACA TCCCCATCAT AACTGAACTG
    GTGAACGACT CCAACGTTCA GTTTTTGGAC CAAGACGACG ATGACGACCC AGACACTGAG
    CTCTACCTGA CCCAGCCGTT CGCCTGTGGT ACCGCATTCG CCGTCAGCGT GTTGGACTCC
    CTGATGAGTG CGACATACTT CAATGATAAT ATCCTCACCC TGATCCGGAC GCTGGTCACG
    GGAGGCGCCA CGCCGGAGCT GGAGGCCCTG TTGGCTGAGG AGAACGCTCT GAGAGGTGGC
    TACAGCACGC CGCAGACGCT GGCTAACAGG GACAGGTGTC GGGTCGCCCA GCTGGCCCTC
    TACGATGGGC CCTTCGCAGA CTTGGGGGAC GGAGGTTGCT ATGGAGACTT GTTCTGTAAA
    GCATTAAAAA CCTACAACAT GCTGTGCTTT GGAATCTATA GATTGCGAGA TGCACATTTA
    GGTGCACCAA GCCAGTGCAC AAAAAGATAT GTCATCACAA ACCCGCCTTA TGAGTTTGAG
    ATGGTGCCCA CGGACCTGAT CTTCTGTCTG ATGCAGTTCG ACCACAACGC GGGCCAGTCC
    CGAGCGAGCC TGTCCCACTC CTCCCATTCG TCCCATTCCT CCAGCAAGAA GAGCTCGTCT
    GTTCACTCCA TCCCAGCCAC CAACCGGCAG AACCGCAGCA GCAAAGCACG TGAAGCCCGT
    GACAAACAGA ATGCAACAAG GATGAATAGA ATGGGCCCAG AAAAGAGATG GTTTACAGAT
    GAAGCAGAAA ACGCATACCC AAGGAACATT CAAATCAAGC CTATGAGCAC TCACATGGCC
    AACCAAGTCA ATCAGTACAA ATCCACTAGC AGTCTGATTC CACCTATCCG AGAAGTCGAA
    GACGAATGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45449
    Clone ID Related Accession (Same CDS sequence) XM_009307246.2 , XM_009307246.3
    Accession Version XM_009307246.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3792bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307246.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAACCA AGATGTCCGT CTACAAGCGA
    ATGAAACTGG CATGTTGTTT TGATTGCGGG CGCTCTGAGC GCGACTGCTC GTGCATGTCA
    GGCAGTGTAC ATAGTAACAT GGACAGCCTT CAGAGGGCCT TCCCACTTTC TTCTGTCTCT
    GTTCATGATT GCTCAACCAG TTTACGTGCC TTCGAAGATG AGCATCCGTC AACACTGTCA
    CCCAAAAAGA AGCAGCGCAA CGGAGGCATG CGAAATTCAC CCAACTGCTC GCCCAAAATG
    ATGAGGCATG ATCCTCTACT CATTCCAGGA AATGAGCAGA TTGAGAGCAT GGATGCTAAT
    GTCAAACGAT ACGACTCTAC TGGAATGTTT CACTGGTGTC CATCCAAAGA AATCGAGAAG
    GTCATTCTGA CACGCAGCGA GGCCTCAATG ACGGTTTTAA GTGGACATGT CGTAGTCTGC
    ATATTTGGAG ATGTCACGTC AGCTTTAGTG GGGCTTCGAA ACTTAGTCAT GCCTTTGAGA
    GCAAGCAACT TCCATTACCA TGAGCTAAAG CCCATTGTGT TTGTGGGCTC GTTAGATTAT
    CTGCGAAGAG AGTGGGAAAC CCTTCACAAC TTCCCCAAAG TCTTCATCTT ACCTGGTACG
    CCATTAAGTC GAGCTGATTT AAGGGCCGTC AACATCAACC TCTGCGACAT GTGCGTTATC
    CTGTCAGCCA ATCAGAACAA TATCGACGAT GCTTCACTGC AGGACAAGGA ATGCATCTTG
    GCATCTCTTA ACATCAAATC TATGCAGTTT GATGACAGCA TTGGGGTCTT GCAGGCTAAT
    TCCCAAGGTT TCACACCTCC AGGGATGGAT AGGTCATCTC CTGATAACAG TCCCGTGCAC
    GGCTTAGTGC GGCAGGCATC CGTCACCACC GGATCAAACA TCCCCATCAT AACTGAACTG
    GTGAACGACT CCAACGTTCA GTTTTTGGAC CAAGACGACG ATGACGACCC AGACACTGAG
    CTCTACCTGA CCCAGCCGTT CGCCTGTGGT ACCGCATTCG CCGTCAGCGT GTTGGACTCC
    CTGATGAGTG CGACATACTT CAATGATAAT ATCCTCACCC TGATCCGGAC GCTGGTCACG
    GGAGGCGCCA CGCCGGAGCT GGAGGCCCTG TTGGCTGAGG AGAACGCTCT GAGAGGTGGC
    TACAGCACGC CGCAGACGCT GGCTAACAGG GACAGGTGTC GGGTCGCCCA GCTGGCCCTC
    TACGATGGGC CCTTCGCAGA CTTGGGGGAC GGAGGTTGCT ATGGAGACTT GTTCTGTAAA
    GCATTAAAAA CCTACAACAT GCTGTGCTTT GGAATCTATA GATTGCGAGA TGCACATTTA
    GGTGCACCAA GCCAGTGCAC AAAAAGATAT GTCATCACAA ACCCGCCTTA TGAGTTTGAG
    ATGGTGCCCA CGGACCTGAT CTTCTGTCTG ATGCAGTTCG ACCACAACGC GGGCCAGTCC
    CGAGCGAGCC TGTCCCACTC CTCCCATTCG TCCCATTCCT CCAGCAAGAA GAGCTCGTCT
    GTTCACTCCA TCCCAGCCAC CAACCGGCAG AACCGCAGCA GCAAAGCACG TGAAGCCCGT
    GACAAACAGA ATGCAACAAG GATGAATAGA ATGGGCCCAG AAAAGAGATG GTTTACAGAT
    GAAGCAGAAA ACGCATACCC AAGGAACATT CAAATCAAGC CTATGAGCAC TCACATGGCC
    AACCAAGTCA ATCAGTACAA ATCCACTAGC AGTCTGATTC CACCTATCCG AGAAGTCGAA
    GACGAATGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305521.1
    CDS676..4467
    Translation

    Target ORF information:

    RefSeq Version XM_009307246.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307246.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAACCA AGATGTCCGT CTACAAGCGA
    ATGAAACTGG CATGTTGTTT TGATTGCGGG CGCTCTGAGC GCGACTGCTC GTGCATGTCA
    GGCAGTGTAC ATAGTAACAT GGACAGCCTT CAGAGGGCCT TCCCACTTTC TTCTGTCTCT
    GTTCATGATT GCTCAACCAG TTTACGTGCC TTCGAAGATG AGCATCCGTC AACACTGTCA
    CCCAAAAAGA AGCAGCGCAA CGGAGGCATG CGAAATTCAC CCAACTGCTC GCCCAAAATG
    ATGAGGCATG ATCCTCTACT CATTCCAGGA AATGAGCAGA TTGAGAGCAT GGATGCTAAT
    GTCAAACGAT ACGACTCTAC TGGAATGTTT CACTGGTGTC CATCCAAAGA AATCGAGAAG
    GTCATTCTGA CACGCAGCGA GGCCTCAATG ACGGTTTTAA GTGGACATGT CGTAGTCTGC
    ATATTTGGAG ATGTCACGTC AGCTTTAGTG GGGCTTCGAA ACTTAGTCAT GCCTTTGAGA
    GCAAGCAACT TCCATTACCA TGAGCTAAAG CCCATTGTGT TTGTGGGCTC GTTAGATTAT
    CTGCGAAGAG AGTGGGAAAC CCTTCACAAC TTCCCCAAAG TCTTCATCTT ACCTGGTACG
    CCATTAAGTC GAGCTGATTT AAGGGCCGTC AACATCAACC TCTGCGACAT GTGCGTTATC
    CTGTCAGCCA ATCAGAACAA TATCGACGAT GCTTCACTGC AGGACAAGGA ATGCATCTTG
    GCATCTCTTA ACATCAAATC TATGCAGTTT GATGACAGCA TTGGGGTCTT GCAGGCTAAT
    TCCCAAGGTT TCACACCTCC AGGGATGGAT AGGTCATCTC CTGATAACAG TCCCGTGCAC
    GGCTTAGTGC GGCAGGCATC CGTCACCACC GGATCAAACA TCCCCATCAT AACTGAACTG
    GTGAACGACT CCAACGTTCA GTTTTTGGAC CAAGACGACG ATGACGACCC AGACACTGAG
    CTCTACCTGA CCCAGCCGTT CGCCTGTGGT ACCGCATTCG CCGTCAGCGT GTTGGACTCC
    CTGATGAGTG CGACATACTT CAATGATAAT ATCCTCACCC TGATCCGGAC GCTGGTCACG
    GGAGGCGCCA CGCCGGAGCT GGAGGCCCTG TTGGCTGAGG AGAACGCTCT GAGAGGTGGC
    TACAGCACGC CGCAGACGCT GGCTAACAGG GACAGGTGTC GGGTCGCCCA GCTGGCCCTC
    TACGATGGGC CCTTCGCAGA CTTGGGGGAC GGAGGTTGCT ATGGAGACTT GTTCTGTAAA
    GCATTAAAAA CCTACAACAT GCTGTGCTTT GGAATCTATA GATTGCGAGA TGCACATTTA
    GGTGCACCAA GCCAGTGCAC AAAAAGATAT GTCATCACAA ACCCGCCTTA TGAGTTTGAG
    ATGGTGCCCA CGGACCTGAT CTTCTGTCTG ATGCAGTTCG ACCACAACGC GGGCCAGTCC
    CGAGCGAGCC TGTCCCACTC CTCCCATTCG TCCCATTCCT CCAGCAAGAA GAGCTCGTCT
    GTTCACTCCA TCCCAGCCAC CAACCGGCAG AACCGCAGCA GCAAAGCACG TGAAGCCCGT
    GACAAACAGA ATGCAACAAG GATGAATAGA ATGGGCCCAG AAAAGAGATG GTTTACAGAT
    GAAGCAGAAA ACGCATACCC AAGGAACATT CAAATCAAGC CTATGAGCAC TCACATGGCC
    AACCAAGTCA ATCAGTACAA ATCCACTAGC AGTCTGATTC CACCTATCCG AGAAGTCGAA
    GACGAATGCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45450
    Clone ID Related Accession (Same CDS sequence) XM_009307247.2 , XM_009307247.3
    Accession Version XM_009307247.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3759bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307247.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT CCAAATATAA GTTTAACGGA
    TATGTCAGAT CACTCGAAGA TGAGCATCCG TCAACACTGT CACCCAAAAA GAAGCAGCGC
    AACGGAGGCA TGCGAAATTC ACCCAACTGC TCGCCCAAAA TGATGAGGCA TGATCCTCTA
    CTCATTCCAG GAAATGAGCA GATTGAGAGC ATGGATGCTA ATGTCAAACG ATACGACTCT
    ACTGGAATGT TTCACTGGTG TCCATCCAAA GAAATCGAGA AGGTCATTCT GACACGCAGC
    GAGGCCTCAA TGACGGTTTT AAGTGGACAT GTCGTAGTCT GCATATTTGG AGATGTCACG
    TCAGCTTTAG TGGGGCTTCG AAACTTAGTC ATGCCTTTGA GAGCAAGCAA CTTCCATTAC
    CATGAGCTAA AGCCCATTGT GTTTGTGGGC TCGTTAGATT ATCTGCGAAG AGAGTGGGAA
    ACCCTTCACA ACTTCCCCAA AGTCTTCATC TTACCTGGTA CGCCATTAAG TCGAGCTGAT
    TTAAGGGCCG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAC
    AATATCGACG ATGCTTCACT GCAGGACAAG GAATGCATCT TGGCATCTCT TAACATCAAA
    TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG TTTCACACCT
    CCAGGGATGG ATAGGTCATC TCCTGATAAC AGTCCCGTGC ACGGCTTAGT GCGGCAGGCA
    TCCGTCACCA CCGGATCAAA CATCCCCATC ATAACTGAAC TGGCTAAGCC TGGCAAACTA
    CTGCCTTTGG TTTCATTCAG TCAGGAAAAA AACAGTGGAA CCAACATACA AATGATAACA
    GAACTGGTGA ACGACTCCAA CGTTCAGTTT TTGGACCAAG ACGACGATGA CGACCCAGAC
    ACTGAGCTCT ACCTGACCCA GCCGTTCGCC TGTGGTACCG CATTCGCCGT CAGCGTGTTG
    GACTCCCTGA TGAGTGCGAC ATACTTCAAT GATAATATCC TCACCCTGAT CCGGACGCTG
    GTCACGGGAG GCGCCACGCC GGAGCTGGAG GCCCTGTTGG CTGAGGAGAA CGCTCTGAGA
    GGTGGCTACA GCACGCCGCA GACGCTGGCT AACAGGGACA GGTGTCGGGT CGCCCAGCTG
    GCCCTCTACG ATGGGCCCTT CGCAGACTTG GGGGACGGAG GTTGCTATGG AGACTTGTTC
    TGTAAAGCAT TAAAAACCTA CAACATGCTG TGCTTTGGAA TCTATAGATT GCGAGATGCA
    CATTTAGGTG CACCAAGCCA GTGCACAAAA AGATATGTCA TCACAAACCC GCCTTATGAG
    TTTGAGATGG TGCCCACGGA CCTGATCTTC TGTCTGATGC AGTTCGACCA CAACGCGGGC
    CAGTCCCGAG CGAGCCTGTC CCACTCCTCC CATTCGTCCC ATTCCTCCAG CAAGAAGAGC
    TCGTCTGTTC ACTCCATCCC AGCCACCAAC CGGCAGAACC GCAGCAGCAA AGCACGTGAA
    GCCCGTGACA AACAGAAAAA AGAGATGGTT TACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305522.1
    CDS662..4420
    Translation

    Target ORF information:

    RefSeq Version XM_009307247.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307247.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT CCAAATATAA GTTTAACGGA
    TATGTCAGAT CACTCGAAGA TGAGCATCCG TCAACACTGT CACCCAAAAA GAAGCAGCGC
    AACGGAGGCA TGCGAAATTC ACCCAACTGC TCGCCCAAAA TGATGAGGCA TGATCCTCTA
    CTCATTCCAG GAAATGAGCA GATTGAGAGC ATGGATGCTA ATGTCAAACG ATACGACTCT
    ACTGGAATGT TTCACTGGTG TCCATCCAAA GAAATCGAGA AGGTCATTCT GACACGCAGC
    GAGGCCTCAA TGACGGTTTT AAGTGGACAT GTCGTAGTCT GCATATTTGG AGATGTCACG
    TCAGCTTTAG TGGGGCTTCG AAACTTAGTC ATGCCTTTGA GAGCAAGCAA CTTCCATTAC
    CATGAGCTAA AGCCCATTGT GTTTGTGGGC TCGTTAGATT ATCTGCGAAG AGAGTGGGAA
    ACCCTTCACA ACTTCCCCAA AGTCTTCATC TTACCTGGTA CGCCATTAAG TCGAGCTGAT
    TTAAGGGCCG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAC
    AATATCGACG ATGCTTCACT GCAGGACAAG GAATGCATCT TGGCATCTCT TAACATCAAA
    TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG TTTCACACCT
    CCAGGGATGG ATAGGTCATC TCCTGATAAC AGTCCCGTGC ACGGCTTAGT GCGGCAGGCA
    TCCGTCACCA CCGGATCAAA CATCCCCATC ATAACTGAAC TGGCTAAGCC TGGCAAACTA
    CTGCCTTTGG TTTCATTCAG TCAGGAAAAA AACAGTGGAA CCAACATACA AATGATAACA
    GAACTGGTGA ACGACTCCAA CGTTCAGTTT TTGGACCAAG ACGACGATGA CGACCCAGAC
    ACTGAGCTCT ACCTGACCCA GCCGTTCGCC TGTGGTACCG CATTCGCCGT CAGCGTGTTG
    GACTCCCTGA TGAGTGCGAC ATACTTCAAT GATAATATCC TCACCCTGAT CCGGACGCTG
    GTCACGGGAG GCGCCACGCC GGAGCTGGAG GCCCTGTTGG CTGAGGAGAA CGCTCTGAGA
    GGTGGCTACA GCACGCCGCA GACGCTGGCT AACAGGGACA GGTGTCGGGT CGCCCAGCTG
    GCCCTCTACG ATGGGCCCTT CGCAGACTTG GGGGACGGAG GTTGCTATGG AGACTTGTTC
    TGTAAAGCAT TAAAAACCTA CAACATGCTG TGCTTTGGAA TCTATAGATT GCGAGATGCA
    CATTTAGGTG CACCAAGCCA GTGCACAAAA AGATATGTCA TCACAAACCC GCCTTATGAG
    TTTGAGATGG TGCCCACGGA CCTGATCTTC TGTCTGATGC AGTTCGACCA CAACGCGGGC
    CAGTCCCGAG CGAGCCTGTC CCACTCCTCC CATTCGTCCC ATTCCTCCAG CAAGAAGAGC
    TCGTCTGTTC ACTCCATCCC AGCCACCAAC CGGCAGAACC GCAGCAGCAA AGCACGTGAA
    GCCCGTGACA AACAGAAAAA AGAGATGGTT TACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45450
    Clone ID Related Accession (Same CDS sequence) XM_009307247.2 , XM_009307247.3
    Accession Version XM_009307247.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3759bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307247.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT CCAAATATAA GTTTAACGGA
    TATGTCAGAT CACTCGAAGA TGAGCATCCG TCAACACTGT CACCCAAAAA GAAGCAGCGC
    AACGGAGGCA TGCGAAATTC ACCCAACTGC TCGCCCAAAA TGATGAGGCA TGATCCTCTA
    CTCATTCCAG GAAATGAGCA GATTGAGAGC ATGGATGCTA ATGTCAAACG ATACGACTCT
    ACTGGAATGT TTCACTGGTG TCCATCCAAA GAAATCGAGA AGGTCATTCT GACACGCAGC
    GAGGCCTCAA TGACGGTTTT AAGTGGACAT GTCGTAGTCT GCATATTTGG AGATGTCACG
    TCAGCTTTAG TGGGGCTTCG AAACTTAGTC ATGCCTTTGA GAGCAAGCAA CTTCCATTAC
    CATGAGCTAA AGCCCATTGT GTTTGTGGGC TCGTTAGATT ATCTGCGAAG AGAGTGGGAA
    ACCCTTCACA ACTTCCCCAA AGTCTTCATC TTACCTGGTA CGCCATTAAG TCGAGCTGAT
    TTAAGGGCCG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAC
    AATATCGACG ATGCTTCACT GCAGGACAAG GAATGCATCT TGGCATCTCT TAACATCAAA
    TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG TTTCACACCT
    CCAGGGATGG ATAGGTCATC TCCTGATAAC AGTCCCGTGC ACGGCTTAGT GCGGCAGGCA
    TCCGTCACCA CCGGATCAAA CATCCCCATC ATAACTGAAC TGGCTAAGCC TGGCAAACTA
    CTGCCTTTGG TTTCATTCAG TCAGGAAAAA AACAGTGGAA CCAACATACA AATGATAACA
    GAACTGGTGA ACGACTCCAA CGTTCAGTTT TTGGACCAAG ACGACGATGA CGACCCAGAC
    ACTGAGCTCT ACCTGACCCA GCCGTTCGCC TGTGGTACCG CATTCGCCGT CAGCGTGTTG
    GACTCCCTGA TGAGTGCGAC ATACTTCAAT GATAATATCC TCACCCTGAT CCGGACGCTG
    GTCACGGGAG GCGCCACGCC GGAGCTGGAG GCCCTGTTGG CTGAGGAGAA CGCTCTGAGA
    GGTGGCTACA GCACGCCGCA GACGCTGGCT AACAGGGACA GGTGTCGGGT CGCCCAGCTG
    GCCCTCTACG ATGGGCCCTT CGCAGACTTG GGGGACGGAG GTTGCTATGG AGACTTGTTC
    TGTAAAGCAT TAAAAACCTA CAACATGCTG TGCTTTGGAA TCTATAGATT GCGAGATGCA
    CATTTAGGTG CACCAAGCCA GTGCACAAAA AGATATGTCA TCACAAACCC GCCTTATGAG
    TTTGAGATGG TGCCCACGGA CCTGATCTTC TGTCTGATGC AGTTCGACCA CAACGCGGGC
    CAGTCCCGAG CGAGCCTGTC CCACTCCTCC CATTCGTCCC ATTCCTCCAG CAAGAAGAGC
    TCGTCTGTTC ACTCCATCCC AGCCACCAAC CGGCAGAACC GCAGCAGCAA AGCACGTGAA
    GCCCGTGACA AACAGAAAAA AGAGATGGTT TACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305522.1
    CDS679..4437
    Translation

    Target ORF information:

    RefSeq Version XM_009307247.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307247.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT CCAAATATAA GTTTAACGGA
    TATGTCAGAT CACTCGAAGA TGAGCATCCG TCAACACTGT CACCCAAAAA GAAGCAGCGC
    AACGGAGGCA TGCGAAATTC ACCCAACTGC TCGCCCAAAA TGATGAGGCA TGATCCTCTA
    CTCATTCCAG GAAATGAGCA GATTGAGAGC ATGGATGCTA ATGTCAAACG ATACGACTCT
    ACTGGAATGT TTCACTGGTG TCCATCCAAA GAAATCGAGA AGGTCATTCT GACACGCAGC
    GAGGCCTCAA TGACGGTTTT AAGTGGACAT GTCGTAGTCT GCATATTTGG AGATGTCACG
    TCAGCTTTAG TGGGGCTTCG AAACTTAGTC ATGCCTTTGA GAGCAAGCAA CTTCCATTAC
    CATGAGCTAA AGCCCATTGT GTTTGTGGGC TCGTTAGATT ATCTGCGAAG AGAGTGGGAA
    ACCCTTCACA ACTTCCCCAA AGTCTTCATC TTACCTGGTA CGCCATTAAG TCGAGCTGAT
    TTAAGGGCCG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAC
    AATATCGACG ATGCTTCACT GCAGGACAAG GAATGCATCT TGGCATCTCT TAACATCAAA
    TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG TTTCACACCT
    CCAGGGATGG ATAGGTCATC TCCTGATAAC AGTCCCGTGC ACGGCTTAGT GCGGCAGGCA
    TCCGTCACCA CCGGATCAAA CATCCCCATC ATAACTGAAC TGGCTAAGCC TGGCAAACTA
    CTGCCTTTGG TTTCATTCAG TCAGGAAAAA AACAGTGGAA CCAACATACA AATGATAACA
    GAACTGGTGA ACGACTCCAA CGTTCAGTTT TTGGACCAAG ACGACGATGA CGACCCAGAC
    ACTGAGCTCT ACCTGACCCA GCCGTTCGCC TGTGGTACCG CATTCGCCGT CAGCGTGTTG
    GACTCCCTGA TGAGTGCGAC ATACTTCAAT GATAATATCC TCACCCTGAT CCGGACGCTG
    GTCACGGGAG GCGCCACGCC GGAGCTGGAG GCCCTGTTGG CTGAGGAGAA CGCTCTGAGA
    GGTGGCTACA GCACGCCGCA GACGCTGGCT AACAGGGACA GGTGTCGGGT CGCCCAGCTG
    GCCCTCTACG ATGGGCCCTT CGCAGACTTG GGGGACGGAG GTTGCTATGG AGACTTGTTC
    TGTAAAGCAT TAAAAACCTA CAACATGCTG TGCTTTGGAA TCTATAGATT GCGAGATGCA
    CATTTAGGTG CACCAAGCCA GTGCACAAAA AGATATGTCA TCACAAACCC GCCTTATGAG
    TTTGAGATGG TGCCCACGGA CCTGATCTTC TGTCTGATGC AGTTCGACCA CAACGCGGGC
    CAGTCCCGAG CGAGCCTGTC CCACTCCTCC CATTCGTCCC ATTCCTCCAG CAAGAAGAGC
    TCGTCTGTTC ACTCCATCCC AGCCACCAAC CGGCAGAACC GCAGCAGCAA AGCACGTGAA
    GCCCGTGACA AACAGAAAAA AGAGATGGTT TACAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45451
    Clone ID Related Accession (Same CDS sequence) XM_009307248.2 , XM_009307248.3
    Accession Version XM_009307248.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3759bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X10
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307248.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT CCAAATATAA GTTTAACGGA
    TATGTCAGAT CACTCGAAGA TGAGCATCCG TCAACACTGT CACCCAAAAA GAAGCAGCGC
    AACGGAGGCA TGCGAAATTC ACCCAACTGC TCGCCCAAAA TGATGAGGCA TGATCCTCTA
    CTCATTCCAG GAAATGAGCA GATTGAGAGC ATGGATGCTA ATGTCAAACG ATACGACTCT
    ACTGGAATGT TTCACTGGTG TCCATCCAAA GAAATCGAGA AGGTCATTCT GACACGCAGC
    GAGGCCTCAA TGACGGTTTT AAGTGGACAT GTCGTAGTCT GCATATTTGG AGATGTCACG
    TCAGCTTTAG TGGGGCTTCG AAACTTAGTC ATGCCTTTGA GAGCAAGCAA CTTCCATTAC
    CATGAGCTAA AGCCCATTGT GTTTGTGGGC TCGTTAGATT ATCTGCGAAG AGAGTGGGAA
    ACCCTTCACA ACTTCCCCAA AGTCTTCATC TTACCTGGTA CGCCATTAAG TCGAGCTGAT
    TTAAGGGCCG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAC
    AATATCGACG ATGCTTCACT GCAGGACAAG GAATGCATCT TGGCATCTCT TAACATCAAA
    TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG TTTCACACCT
    CCAGGGATGG ATAGGTCATC TCCTGATAAC AGTCCCGTGC ACGGCTTAGT GCGGCAGGCA
    TCCGTCACCA CCGGATCAAA CATCCCCATC ATAACTGAAC TGGCTAAGCC TGGCAAACTA
    CTGCCTTTGG TTTCATTCAG TCAGGAAAAA AACAGTGGAA CCAACATACA AATGATAACA
    GAACTGGTGA ACGACTCCAA CGTTCAGTTT TTGGACCAAG ACGACGATGA CGACCCAGAC
    ACTGAGCTCT ACCTGACCCA GCCGTTCGCC TGTGGTACCG CATTCGCCGT CAGCGTGTTG
    GACTCCCTGA TGAGTGCGAC ATACTTCAAT GATAATATCC TCACCCTGAT CCGGACGCTG
    GTCACGGGAG GCGCCACGCC GGAGCTGGAG GCCCTGTTGG CTGAGGAGAA CGCTCTGAGA
    GGTGGCTACA GCACGCCGCA GACGCTGGCT AACAGGGACA GGTGTCGGGT CGCCCAGCTG
    GCCCTCTACG ATGGGCCCTT CGCAGACTTG GGGGACGGAG GTTGCTATGG AGACTTGTTC
    TGTAAAGCAT TAAAAACCTA CAACATGCTG TGCTTTGGAA TCTATAGATT GCGAGATGCA
    CATTTAGGTG CACCAAGCCA GTGCACAAAA AGATATGTCA TCACAAACCC GCCTTATGAG
    TTTGAGATGG TGCCCACGGA CCTGATCTTC TGTCTGATGC AGTTCGACCA CAACGCGGGC
    CAGTCCCGAG CGAGCCTGTC CCACTCCTCC CATTCGTCCC ATTCCTCCAG CAAGAAGAGC
    TCGTCTGTTC ACTCCATCCC AGCCACCAAC CGGCAGAACC GCAGCAGCAA AGCACGTGAA
    GCCCGTGACA AACAGAAAAA AGAGATGGTG TTTAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305523.1
    CDS665..4423
    Translation

    Target ORF information:

    RefSeq Version XM_009307248.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307248.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT CCAAATATAA GTTTAACGGA
    TATGTCAGAT CACTCGAAGA TGAGCATCCG TCAACACTGT CACCCAAAAA GAAGCAGCGC
    AACGGAGGCA TGCGAAATTC ACCCAACTGC TCGCCCAAAA TGATGAGGCA TGATCCTCTA
    CTCATTCCAG GAAATGAGCA GATTGAGAGC ATGGATGCTA ATGTCAAACG ATACGACTCT
    ACTGGAATGT TTCACTGGTG TCCATCCAAA GAAATCGAGA AGGTCATTCT GACACGCAGC
    GAGGCCTCAA TGACGGTTTT AAGTGGACAT GTCGTAGTCT GCATATTTGG AGATGTCACG
    TCAGCTTTAG TGGGGCTTCG AAACTTAGTC ATGCCTTTGA GAGCAAGCAA CTTCCATTAC
    CATGAGCTAA AGCCCATTGT GTTTGTGGGC TCGTTAGATT ATCTGCGAAG AGAGTGGGAA
    ACCCTTCACA ACTTCCCCAA AGTCTTCATC TTACCTGGTA CGCCATTAAG TCGAGCTGAT
    TTAAGGGCCG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAC
    AATATCGACG ATGCTTCACT GCAGGACAAG GAATGCATCT TGGCATCTCT TAACATCAAA
    TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG TTTCACACCT
    CCAGGGATGG ATAGGTCATC TCCTGATAAC AGTCCCGTGC ACGGCTTAGT GCGGCAGGCA
    TCCGTCACCA CCGGATCAAA CATCCCCATC ATAACTGAAC TGGCTAAGCC TGGCAAACTA
    CTGCCTTTGG TTTCATTCAG TCAGGAAAAA AACAGTGGAA CCAACATACA AATGATAACA
    GAACTGGTGA ACGACTCCAA CGTTCAGTTT TTGGACCAAG ACGACGATGA CGACCCAGAC
    ACTGAGCTCT ACCTGACCCA GCCGTTCGCC TGTGGTACCG CATTCGCCGT CAGCGTGTTG
    GACTCCCTGA TGAGTGCGAC ATACTTCAAT GATAATATCC TCACCCTGAT CCGGACGCTG
    GTCACGGGAG GCGCCACGCC GGAGCTGGAG GCCCTGTTGG CTGAGGAGAA CGCTCTGAGA
    GGTGGCTACA GCACGCCGCA GACGCTGGCT AACAGGGACA GGTGTCGGGT CGCCCAGCTG
    GCCCTCTACG ATGGGCCCTT CGCAGACTTG GGGGACGGAG GTTGCTATGG AGACTTGTTC
    TGTAAAGCAT TAAAAACCTA CAACATGCTG TGCTTTGGAA TCTATAGATT GCGAGATGCA
    CATTTAGGTG CACCAAGCCA GTGCACAAAA AGATATGTCA TCACAAACCC GCCTTATGAG
    TTTGAGATGG TGCCCACGGA CCTGATCTTC TGTCTGATGC AGTTCGACCA CAACGCGGGC
    CAGTCCCGAG CGAGCCTGTC CCACTCCTCC CATTCGTCCC ATTCCTCCAG CAAGAAGAGC
    TCGTCTGTTC ACTCCATCCC AGCCACCAAC CGGCAGAACC GCAGCAGCAA AGCACGTGAA
    GCCCGTGACA AACAGAAAAA AGAGATGGTG TTTAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45451
    Clone ID Related Accession (Same CDS sequence) XM_009307248.2 , XM_009307248.3
    Accession Version XM_009307248.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3759bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307248.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT CCAAATATAA GTTTAACGGA
    TATGTCAGAT CACTCGAAGA TGAGCATCCG TCAACACTGT CACCCAAAAA GAAGCAGCGC
    AACGGAGGCA TGCGAAATTC ACCCAACTGC TCGCCCAAAA TGATGAGGCA TGATCCTCTA
    CTCATTCCAG GAAATGAGCA GATTGAGAGC ATGGATGCTA ATGTCAAACG ATACGACTCT
    ACTGGAATGT TTCACTGGTG TCCATCCAAA GAAATCGAGA AGGTCATTCT GACACGCAGC
    GAGGCCTCAA TGACGGTTTT AAGTGGACAT GTCGTAGTCT GCATATTTGG AGATGTCACG
    TCAGCTTTAG TGGGGCTTCG AAACTTAGTC ATGCCTTTGA GAGCAAGCAA CTTCCATTAC
    CATGAGCTAA AGCCCATTGT GTTTGTGGGC TCGTTAGATT ATCTGCGAAG AGAGTGGGAA
    ACCCTTCACA ACTTCCCCAA AGTCTTCATC TTACCTGGTA CGCCATTAAG TCGAGCTGAT
    TTAAGGGCCG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAC
    AATATCGACG ATGCTTCACT GCAGGACAAG GAATGCATCT TGGCATCTCT TAACATCAAA
    TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG TTTCACACCT
    CCAGGGATGG ATAGGTCATC TCCTGATAAC AGTCCCGTGC ACGGCTTAGT GCGGCAGGCA
    TCCGTCACCA CCGGATCAAA CATCCCCATC ATAACTGAAC TGGCTAAGCC TGGCAAACTA
    CTGCCTTTGG TTTCATTCAG TCAGGAAAAA AACAGTGGAA CCAACATACA AATGATAACA
    GAACTGGTGA ACGACTCCAA CGTTCAGTTT TTGGACCAAG ACGACGATGA CGACCCAGAC
    ACTGAGCTCT ACCTGACCCA GCCGTTCGCC TGTGGTACCG CATTCGCCGT CAGCGTGTTG
    GACTCCCTGA TGAGTGCGAC ATACTTCAAT GATAATATCC TCACCCTGAT CCGGACGCTG
    GTCACGGGAG GCGCCACGCC GGAGCTGGAG GCCCTGTTGG CTGAGGAGAA CGCTCTGAGA
    GGTGGCTACA GCACGCCGCA GACGCTGGCT AACAGGGACA GGTGTCGGGT CGCCCAGCTG
    GCCCTCTACG ATGGGCCCTT CGCAGACTTG GGGGACGGAG GTTGCTATGG AGACTTGTTC
    TGTAAAGCAT TAAAAACCTA CAACATGCTG TGCTTTGGAA TCTATAGATT GCGAGATGCA
    CATTTAGGTG CACCAAGCCA GTGCACAAAA AGATATGTCA TCACAAACCC GCCTTATGAG
    TTTGAGATGG TGCCCACGGA CCTGATCTTC TGTCTGATGC AGTTCGACCA CAACGCGGGC
    CAGTCCCGAG CGAGCCTGTC CCACTCCTCC CATTCGTCCC ATTCCTCCAG CAAGAAGAGC
    TCGTCTGTTC ACTCCATCCC AGCCACCAAC CGGCAGAACC GCAGCAGCAA AGCACGTGAA
    GCCCGTGACA AACAGAAAAA AGAGATGGTG TTTAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305523.1
    CDS682..4440
    Translation

    Target ORF information:

    RefSeq Version XM_009307248.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307248.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT CCAAATATAA GTTTAACGGA
    TATGTCAGAT CACTCGAAGA TGAGCATCCG TCAACACTGT CACCCAAAAA GAAGCAGCGC
    AACGGAGGCA TGCGAAATTC ACCCAACTGC TCGCCCAAAA TGATGAGGCA TGATCCTCTA
    CTCATTCCAG GAAATGAGCA GATTGAGAGC ATGGATGCTA ATGTCAAACG ATACGACTCT
    ACTGGAATGT TTCACTGGTG TCCATCCAAA GAAATCGAGA AGGTCATTCT GACACGCAGC
    GAGGCCTCAA TGACGGTTTT AAGTGGACAT GTCGTAGTCT GCATATTTGG AGATGTCACG
    TCAGCTTTAG TGGGGCTTCG AAACTTAGTC ATGCCTTTGA GAGCAAGCAA CTTCCATTAC
    CATGAGCTAA AGCCCATTGT GTTTGTGGGC TCGTTAGATT ATCTGCGAAG AGAGTGGGAA
    ACCCTTCACA ACTTCCCCAA AGTCTTCATC TTACCTGGTA CGCCATTAAG TCGAGCTGAT
    TTAAGGGCCG TCAACATCAA CCTCTGCGAC ATGTGCGTTA TCCTGTCAGC CAATCAGAAC
    AATATCGACG ATGCTTCACT GCAGGACAAG GAATGCATCT TGGCATCTCT TAACATCAAA
    TCTATGCAGT TTGATGACAG CATTGGGGTC TTGCAGGCTA ATTCCCAAGG TTTCACACCT
    CCAGGGATGG ATAGGTCATC TCCTGATAAC AGTCCCGTGC ACGGCTTAGT GCGGCAGGCA
    TCCGTCACCA CCGGATCAAA CATCCCCATC ATAACTGAAC TGGCTAAGCC TGGCAAACTA
    CTGCCTTTGG TTTCATTCAG TCAGGAAAAA AACAGTGGAA CCAACATACA AATGATAACA
    GAACTGGTGA ACGACTCCAA CGTTCAGTTT TTGGACCAAG ACGACGATGA CGACCCAGAC
    ACTGAGCTCT ACCTGACCCA GCCGTTCGCC TGTGGTACCG CATTCGCCGT CAGCGTGTTG
    GACTCCCTGA TGAGTGCGAC ATACTTCAAT GATAATATCC TCACCCTGAT CCGGACGCTG
    GTCACGGGAG GCGCCACGCC GGAGCTGGAG GCCCTGTTGG CTGAGGAGAA CGCTCTGAGA
    GGTGGCTACA GCACGCCGCA GACGCTGGCT AACAGGGACA GGTGTCGGGT CGCCCAGCTG
    GCCCTCTACG ATGGGCCCTT CGCAGACTTG GGGGACGGAG GTTGCTATGG AGACTTGTTC
    TGTAAAGCAT TAAAAACCTA CAACATGCTG TGCTTTGGAA TCTATAGATT GCGAGATGCA
    CATTTAGGTG CACCAAGCCA GTGCACAAAA AGATATGTCA TCACAAACCC GCCTTATGAG
    TTTGAGATGG TGCCCACGGA CCTGATCTTC TGTCTGATGC AGTTCGACCA CAACGCGGGC
    CAGTCCCGAG CGAGCCTGTC CCACTCCTCC CATTCGTCCC ATTCCTCCAG CAAGAAGAGC
    TCGTCTGTTC ACTCCATCCC AGCCACCAAC CGGCAGAACC GCAGCAGCAA AGCACGTGAA
    GCCCGTGACA AACAGAAAAA AGAGATGGTG TTTAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45452
    Clone ID Related Accession (Same CDS sequence) XM_009307250.2 , XM_009307250.3
    Accession Version XM_009307250.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X16
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307250.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT TCGAAGATGA GCATCCGTCA
    ACACTGTCAC CCAAAAAGAA GCAGCGCAAC GGAGGCATGC GAAATTCACC CAACTGCTCG
    CCCAAAATGA TGAGGCATGA TCCTCTACTC ATTCCAGGAA ATGAGCAGAT TGAGAGCATG
    GATGCTAATG TCAAACGATA CGACTCTACT GGAATGTTTC ACTGGTGTCC ATCCAAAGAA
    ATCGAGAAGG TCATTCTGAC ACGCAGCGAG GCCTCAATGA CGGTTTTAAG TGGACATGTC
    GTAGTCTGCA TATTTGGAGA TGTCACGTCA GCTTTAGTGG GGCTTCGAAA CTTAGTCATG
    CCTTTGAGAG CAAGCAACTT CCATTACCAT GAGCTAAAGC CCATTGTGTT TGTGGGCTCG
    TTAGATTATC TGCGAAGAGA GTGGGAAACC CTTCACAACT TCCCCAAAGT CTTCATCTTA
    CCTGGTACGC CATTAAGTCG AGCTGATTTA AGGGCCGTCA ACATCAACCT CTGCGACATG
    TGCGTTATCC TGTCAGCCAA TCAGAACAAT ATCGACGATG CTTCACTGCA GGACAAGGAA
    TGCATCTTGG CATCTCTTAA CATCAAATCT ATGCAGTTTG ATGACAGCAT TGGGGTCTTG
    CAGGCTAATT CCCAAGGTTT CACACCTCCA GGGATGGATA GGTCATCTCC TGATAACAGT
    CCCGTGCACG GCTTAGTGCG GCAGGCATCC GTCACCACCG GATCAAACAT CCCCATCATA
    ACTGAACTGG TGAACGACTC CAACGTTCAG TTTTTGGACC AAGACGACGA TGACGACCCA
    GACACTGAGC TCTACCTGAC CCAGCCGTTC GCCTGTGGTA CCGCATTCGC CGTCAGCGTG
    TTGGACTCCC TGATGAGTGC GACATACTTC AATGATAATA TCCTCACCCT GATCCGGACG
    CTGGTCACGG GAGGCGCCAC GCCGGAGCTG GAGGCCCTGT TGGCTGAGGA GAACGCTCTG
    AGAGGTGGCT ACAGCACGCC GCAGACGCTG GCTAACAGGG ACAGGTGTCG GGTCGCCCAG
    CTGGCCCTCT ACGATGGGCC CTTCGCAGAC TTGGGGGACG GAGGTTGCTA TGGAGACTTG
    TTCTGTAAAG CATTAAAAAC CTACAACATG CTGTGCTTTG GAATCTATAG ATTGCGAGAT
    GCACATTTAG GTGCACCAAG CCAGTGCACA AAAAGATATG TCATCACAAA CCCGCCTTAT
    GAGTTTGAGA TGGTGCCCAC GGACCTGATC TTCTGTCTGA TGCAGTTCGA CCACAACGCG
    GGCCAGTCCC GAGCGAGCCT GTCCCACTCC TCCCATTCGT CCCATTCCTC CAGCAAGAAG
    AGCTCGTCTG TTCACTCCAT CCCAGCCACC AACCGGCAGA ACCGCAGCAG CAAAGCACGT
    GAAGCCCGTG ACAAACAGAA AAAAGAGATG GTTTACAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305525.1
    CDS656..4297
    Translation

    Target ORF information:

    RefSeq Version XM_009307250.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307250.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT TCGAAGATGA GCATCCGTCA
    ACACTGTCAC CCAAAAAGAA GCAGCGCAAC GGAGGCATGC GAAATTCACC CAACTGCTCG
    CCCAAAATGA TGAGGCATGA TCCTCTACTC ATTCCAGGAA ATGAGCAGAT TGAGAGCATG
    GATGCTAATG TCAAACGATA CGACTCTACT GGAATGTTTC ACTGGTGTCC ATCCAAAGAA
    ATCGAGAAGG TCATTCTGAC ACGCAGCGAG GCCTCAATGA CGGTTTTAAG TGGACATGTC
    GTAGTCTGCA TATTTGGAGA TGTCACGTCA GCTTTAGTGG GGCTTCGAAA CTTAGTCATG
    CCTTTGAGAG CAAGCAACTT CCATTACCAT GAGCTAAAGC CCATTGTGTT TGTGGGCTCG
    TTAGATTATC TGCGAAGAGA GTGGGAAACC CTTCACAACT TCCCCAAAGT CTTCATCTTA
    CCTGGTACGC CATTAAGTCG AGCTGATTTA AGGGCCGTCA ACATCAACCT CTGCGACATG
    TGCGTTATCC TGTCAGCCAA TCAGAACAAT ATCGACGATG CTTCACTGCA GGACAAGGAA
    TGCATCTTGG CATCTCTTAA CATCAAATCT ATGCAGTTTG ATGACAGCAT TGGGGTCTTG
    CAGGCTAATT CCCAAGGTTT CACACCTCCA GGGATGGATA GGTCATCTCC TGATAACAGT
    CCCGTGCACG GCTTAGTGCG GCAGGCATCC GTCACCACCG GATCAAACAT CCCCATCATA
    ACTGAACTGG TGAACGACTC CAACGTTCAG TTTTTGGACC AAGACGACGA TGACGACCCA
    GACACTGAGC TCTACCTGAC CCAGCCGTTC GCCTGTGGTA CCGCATTCGC CGTCAGCGTG
    TTGGACTCCC TGATGAGTGC GACATACTTC AATGATAATA TCCTCACCCT GATCCGGACG
    CTGGTCACGG GAGGCGCCAC GCCGGAGCTG GAGGCCCTGT TGGCTGAGGA GAACGCTCTG
    AGAGGTGGCT ACAGCACGCC GCAGACGCTG GCTAACAGGG ACAGGTGTCG GGTCGCCCAG
    CTGGCCCTCT ACGATGGGCC CTTCGCAGAC TTGGGGGACG GAGGTTGCTA TGGAGACTTG
    TTCTGTAAAG CATTAAAAAC CTACAACATG CTGTGCTTTG GAATCTATAG ATTGCGAGAT
    GCACATTTAG GTGCACCAAG CCAGTGCACA AAAAGATATG TCATCACAAA CCCGCCTTAT
    GAGTTTGAGA TGGTGCCCAC GGACCTGATC TTCTGTCTGA TGCAGTTCGA CCACAACGCG
    GGCCAGTCCC GAGCGAGCCT GTCCCACTCC TCCCATTCGT CCCATTCCTC CAGCAAGAAG
    AGCTCGTCTG TTCACTCCAT CCCAGCCACC AACCGGCAGA ACCGCAGCAG CAAAGCACGT
    GAAGCCCGTG ACAAACAGAA AAAAGAGATG GTTTACAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45452
    Clone ID Related Accession (Same CDS sequence) XM_009307250.2 , XM_009307250.3
    Accession Version XM_009307250.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3642bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product calcium-activated potassium channel subunit alpha-1 isoform X18
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307250.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT TCGAAGATGA GCATCCGTCA
    ACACTGTCAC CCAAAAAGAA GCAGCGCAAC GGAGGCATGC GAAATTCACC CAACTGCTCG
    CCCAAAATGA TGAGGCATGA TCCTCTACTC ATTCCAGGAA ATGAGCAGAT TGAGAGCATG
    GATGCTAATG TCAAACGATA CGACTCTACT GGAATGTTTC ACTGGTGTCC ATCCAAAGAA
    ATCGAGAAGG TCATTCTGAC ACGCAGCGAG GCCTCAATGA CGGTTTTAAG TGGACATGTC
    GTAGTCTGCA TATTTGGAGA TGTCACGTCA GCTTTAGTGG GGCTTCGAAA CTTAGTCATG
    CCTTTGAGAG CAAGCAACTT CCATTACCAT GAGCTAAAGC CCATTGTGTT TGTGGGCTCG
    TTAGATTATC TGCGAAGAGA GTGGGAAACC CTTCACAACT TCCCCAAAGT CTTCATCTTA
    CCTGGTACGC CATTAAGTCG AGCTGATTTA AGGGCCGTCA ACATCAACCT CTGCGACATG
    TGCGTTATCC TGTCAGCCAA TCAGAACAAT ATCGACGATG CTTCACTGCA GGACAAGGAA
    TGCATCTTGG CATCTCTTAA CATCAAATCT ATGCAGTTTG ATGACAGCAT TGGGGTCTTG
    CAGGCTAATT CCCAAGGTTT CACACCTCCA GGGATGGATA GGTCATCTCC TGATAACAGT
    CCCGTGCACG GCTTAGTGCG GCAGGCATCC GTCACCACCG GATCAAACAT CCCCATCATA
    ACTGAACTGG TGAACGACTC CAACGTTCAG TTTTTGGACC AAGACGACGA TGACGACCCA
    GACACTGAGC TCTACCTGAC CCAGCCGTTC GCCTGTGGTA CCGCATTCGC CGTCAGCGTG
    TTGGACTCCC TGATGAGTGC GACATACTTC AATGATAATA TCCTCACCCT GATCCGGACG
    CTGGTCACGG GAGGCGCCAC GCCGGAGCTG GAGGCCCTGT TGGCTGAGGA GAACGCTCTG
    AGAGGTGGCT ACAGCACGCC GCAGACGCTG GCTAACAGGG ACAGGTGTCG GGTCGCCCAG
    CTGGCCCTCT ACGATGGGCC CTTCGCAGAC TTGGGGGACG GAGGTTGCTA TGGAGACTTG
    TTCTGTAAAG CATTAAAAAC CTACAACATG CTGTGCTTTG GAATCTATAG ATTGCGAGAT
    GCACATTTAG GTGCACCAAG CCAGTGCACA AAAAGATATG TCATCACAAA CCCGCCTTAT
    GAGTTTGAGA TGGTGCCCAC GGACCTGATC TTCTGTCTGA TGCAGTTCGA CCACAACGCG
    GGCCAGTCCC GAGCGAGCCT GTCCCACTCC TCCCATTCGT CCCATTCCTC CAGCAAGAAG
    AGCTCGTCTG TTCACTCCAT CCCAGCCACC AACCGGCAGA ACCGCAGCAG CAAAGCACGT
    GAAGCCCGTG ACAAACAGAA AAAAGAGATG GTTTACAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305525.1
    CDS676..4317
    Translation

    Target ORF information:

    RefSeq Version XM_009307250.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio potassium large conductance calcium-activated channel, subfamily M, alpha member 1a (kcnma1a), transcript variant X18, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307250.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGCTAGCTG TGGCAGATGG CAACGGATCC TATGGCAGCA GCAGCTCTAG AATGAGTAAT 
    AATATCAATT TTAATAAAAA TCCGGACTCT TCTGTCTCTA TATCGAAGAT GGACGTGATC
    ATCCCGTTCA CACCAGACGT ACCCTGTGAC AACAATGGTC AGCGCATGTG GTGGGCTTTC
    CTCGCCTCAT CTATGGTGAC GTTCTTCGGG GGTCTCTTCA TCATCCTCCT GTGGAGGACC
    CTCAAGTACC TGTGGACTGT CTGCTGCCAT TGCAACATCA AAAACAAGGA GGCACAGAAG
    GTGAATAACC CTATTACTAT TCAGGCTGAT GGCACAACAA AGACTGGGAA TGAAAAAGAA
    GAAGCCCCAG CCAGTGAAGT GGGCTGGATG ACCTCAGTCA AAGACTGGGC CGGGGTCATG
    ATCTCAGCAC AGACCCTCAC CGGCAGAGTT TTGGTGGTGT TGGTCTTTGC ACTCAGCATC
    GGAGCTCTGG GAATATACTT CATAGACTCT TCGGATCCTA TAGAATCCTG TCAGAATTTC
    TACAAAGACT TCACGTTACA AATTGACATG GCATTCAACG TGTTCTTCTT ACTCTACTTT
    GGTCTGCGCT TCATAGCAGC CAATGATAAG CTGTGGTTCT GGCTGGAGGT CAACTCAGTG
    GTGGACTTCT TCACCGTGCC TCCCGTGTTT GTGTCAGTGT ACCTGAACAG GAGTTGGCTT
    GGTCTGAGGT TTCTAAGAGC CTTGAGATTG ATACAGTTCT CAGAAATTTT ACAGTTTTTG
    AATATCTTAA AAACAAGTAA TTCCATCAAG CTGGTGAACC TGTGTTCCAT CTTCATAAGC
    ACATGGCTCA CTGCTGCAGG ATTCATTCAT CTGGTGGAAA ACTCAGGGGA TCCATGGGAA
    AACTTCCAAA ACTCTCAACC GCTCTCATAT TGGGAATGTG TGTACTTATT AATGGTCACC
    ATGTCCACGG TGGGATATGG GGACGTCTAT GCAAGAACCA CTCTTGGACG CCTTTTCATG
    GTCTTCTTCA TTCTCGGTGG TCTGGCCATG TTTGCCAGCT ACGTCCCTGA AATCATAGAG
    TTAATAGGAA ACCGCAAGAA ATATGGTGGT TCCTATAGTG CGGTAAATGG CAGAAAGCAT
    ATTGTGGTCT GTGGTCATAT CACGCTCGAA AGTGTTTCCA ACTTCCTTAA AGACTTCCTA
    CACAAGGACA GGGATGATGT CAATGTAGAA ATTGTTTTTC TTCATAATAT TTCCCCTAAT
    CTTGAGCTGG AAGCCTTGTT CAAACGTCAC TTCACTCAGG TGGAATTCTA TCAAGGATCG
    GTCCTCAACC CACATGATCT AGCAAGAGTG AAGATTGAGT CGGCGGACGC TTGCTTGATT
    CTTGCAAACA AATACTGTGC AGACCCCGAT GCTGAGGATG CCTCAAATAT CATGAGAGTG
    ATATCTATCA AGAACTACCA TCCAAAGATC AGAATAATAA CACAAATGTT GCAGTACCAC
    AACAAGGCCC ATCTGCTAAA CATTCCCAGC TGGAACTGGA AGGAGGGAGA TGATGCGATT
    TGCCTGGCCG AGTTGAAGGC AGGCTTCATA GCCCAGAGCT GCCTGGCACA GGGCCTGTCC
    ACCATGCTCG CCAACCTCTT CTCCATGAGG TCATACATCA AGATTGAAGA AGACACATGG
    CAGAAGTATT ATCTGGAAGG AGTAGCCAAT GAAATGTACA CTGAGTACCT GTCTAGTGCC
    TTTGTCGGCC TTTCATTTCC TACAGTTTGT GAGCTGTGCT ACGTGAAGCT CAAGTTGCTG
    TTGATCGCTA TTGAGTACAA GTCAGAGCAA AGGGAAAGCA GAAGCAGAAA GCGTATTCTG
    ATCAACCCGG GAAATCACGT TAAAATGCAG GAGGGGACAC TGGGATTCTT TATTGCCAGT
    GATGCCAAAG AAGTGAAAAG AGCATTTTTC TACTGCAAAG CATGTCATGA TGACATCACA
    GATCCCAAAC GGATTAAAAA GTGTGGCTGC AAGAGAATGA CATATTCCAA GATGTCCGTC
    TACAAGCGAA TGAAACTGGC ATGTTGTTTT GATTGCGGGC GCTCTGAGCG CGACTGCTCG
    TGCATGTCAG GCAGTGTACA TAGTAACATG GACAGCCTTC AGAGGGCCTT CCCACTTTCT
    TCTGTCTCTG TTCATGATTG CTCAACCAGT TTACGTGCCT TCGAAGATGA GCATCCGTCA
    ACACTGTCAC CCAAAAAGAA GCAGCGCAAC GGAGGCATGC GAAATTCACC CAACTGCTCG
    CCCAAAATGA TGAGGCATGA TCCTCTACTC ATTCCAGGAA ATGAGCAGAT TGAGAGCATG
    GATGCTAATG TCAAACGATA CGACTCTACT GGAATGTTTC ACTGGTGTCC ATCCAAAGAA
    ATCGAGAAGG TCATTCTGAC ACGCAGCGAG GCCTCAATGA CGGTTTTAAG TGGACATGTC
    GTAGTCTGCA TATTTGGAGA TGTCACGTCA GCTTTAGTGG GGCTTCGAAA CTTAGTCATG
    CCTTTGAGAG CAAGCAACTT CCATTACCAT GAGCTAAAGC CCATTGTGTT TGTGGGCTCG
    TTAGATTATC TGCGAAGAGA GTGGGAAACC CTTCACAACT TCCCCAAAGT CTTCATCTTA
    CCTGGTACGC CATTAAGTCG AGCTGATTTA AGGGCCGTCA ACATCAACCT CTGCGACATG
    TGCGTTATCC TGTCAGCCAA TCAGAACAAT ATCGACGATG CTTCACTGCA GGACAAGGAA
    TGCATCTTGG CATCTCTTAA CATCAAATCT ATGCAGTTTG ATGACAGCAT TGGGGTCTTG
    CAGGCTAATT CCCAAGGTTT CACACCTCCA GGGATGGATA GGTCATCTCC TGATAACAGT
    CCCGTGCACG GCTTAGTGCG GCAGGCATCC GTCACCACCG GATCAAACAT CCCCATCATA
    ACTGAACTGG TGAACGACTC CAACGTTCAG TTTTTGGACC AAGACGACGA TGACGACCCA
    GACACTGAGC TCTACCTGAC CCAGCCGTTC GCCTGTGGTA CCGCATTCGC CGTCAGCGTG
    TTGGACTCCC TGATGAGTGC GACATACTTC AATGATAATA TCCTCACCCT GATCCGGACG
    CTGGTCACGG GAGGCGCCAC GCCGGAGCTG GAGGCCCTGT TGGCTGAGGA GAACGCTCTG
    AGAGGTGGCT ACAGCACGCC GCAGACGCTG GCTAACAGGG ACAGGTGTCG GGTCGCCCAG
    CTGGCCCTCT ACGATGGGCC CTTCGCAGAC TTGGGGGACG GAGGTTGCTA TGGAGACTTG
    TTCTGTAAAG CATTAAAAAC CTACAACATG CTGTGCTTTG GAATCTATAG ATTGCGAGAT
    GCACATTTAG GTGCACCAAG CCAGTGCACA AAAAGATATG TCATCACAAA CCCGCCTTAT
    GAGTTTGAGA TGGTGCCCAC GGACCTGATC TTCTGTCTGA TGCAGTTCGA CCACAACGCG
    GGCCAGTCCC GAGCGAGCCT GTCCCACTCC TCCCATTCGT CCCATTCCTC CAGCAAGAAG
    AGCTCGTCTG TTCACTCCAT CCCAGCCACC AACCGGCAGA ACCGCAGCAG CAAAGCACGT
    GAAGCCCGTG ACAAACAGAA AAAAGAGATG GTTTACAGAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.