crb2b cDNA ORF clone, Danio rerio(zebrafish)

The following crb2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the crb2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa03735 NM_001045162.1
Latest version!
Danio rerio crumbs cell polarity complex component 2b (crb2b), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
ODa45443 XM_017357360.2
Latest version!
Danio rerio crumbs family member 2b (crb2b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
View Old Accession Versions >>
ODa45443 XM_017357360.1 Danio rerio crumbs family member 2b (crb2b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa03735
    Clone ID Related Accession (Same CDS sequence) NM_001045162.1
    Accession Version NM_001045162.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4377bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-07
    Organism Danio rerio(zebrafish)
    Product crumbs cell polarity complex component 2b precursor
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DQ314737.1. On Sep 1, 2006 this sequence version replaced XM_691852.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ314737.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01765705, SAMN06350654 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGATTTTG GACGGATGTG TTTACAGTAT AAAAGAGCGG TTCTGCTGTC ATTGATGTTC 
    AAGCTGGGTC TGTTCTGCAC CGTCTCATCA GAAGTGTGCC TGTCGTCCCC GTGCCAAAAC
    AACAGCACCT GTGTGCCCAC ATTGGACGGT TACCTGTGCA CCTGTTCCTC CAGCCCACCA
    ATATACACCG GCCCAAATTG TGAGCGTTTA CTGGACCCGT GTGACTCTGC AGGCTGTCCA
    TCCACATGCA TCGCCACTCC AGGAAGCCAA AACTACACCT GTCTGTGTCC TGATGGCTTC
    AGCGGCCCAA ACTGCACCCA AAACATCAAT GAATGTCACA GCAACCCCTG TTCGGGGGTC
    CGGAGCTTCT GTGTGGATGG AGTGAACGGG TATTCGTGTC ACTGTCCCAG CGGGTACAGC
    GGGGCAGACT GCGGGACCAG GGTGCGGGAC TGCTCAGATG ACCCGTGCTT TAATAATGCC
    ACCTGTGTCT GGGCTCCGGA CGGGTACGAG TGCCACTGTG CTGCAGGTTT TCGAGGTCGT
    AACTGTGAGG AGGACATCGA TGAGTGTCTG TCGCAGCCCT GCAGGAATGG GGCGATCTGT
    CAGGACGCTG TGGGGGTCTA CCAGTGCTAC TGCGTGCCTG GATTTCAGGG TTTTCACTGC
    GAGATTGATA TTAATGAGTG CGCATCGCAG CCCTGCGAGA ACAACGGGAC GTGTGTCAAT
    GGCAGAGACA GATACATCTG CGAGTGTCTC TTTGGGTTCA CAGGTCTCAA CTGTGAAGTG
    GACGTGGATG AATGTGAAGC GTCTCCCTGC CAGAACGGCG CCGTCTGCCA TGATCACGTG
    GGTCTGTACA CCTGTGAGTG TGTGCCGGGG TACGAGGGCA TCAACTGTGA GCTGGACATC
    GATGAGTGTG AGAGCTCACC CTGCCGCCAT GGAGGAGTCT GTCTGGATAT GGTCAACGGG
    TATGAGTGTG ACTGCAGTGG CACTGGATTC ATGGGCTTTT TCTGTGATAT TGACATCCCC
    GAGTGTTCTT CTGACCCCTG TCAACATGGC GCCACCTGCC TGGAGGGAGT AAACCACTAC
    ACCTGCATCT GCTGGACAGG TTATGAAGGA GTGAACTGTG AGCTGGATGT GGACGAGTGT
    GTTTCTCAGC CATGTGAGAA TGAAGGCCAG TGTTTCCAGC GCTCAGACTC CAGCCTCTAC
    AGAGTTCTGC CTGAACTGGA CACTGACTTC AGCTTCCAGA GCGCAGCCGG TTACCTGTGC
    CAGTGCGTAT CTGGCTTCAC AGGTGAAAAC TGCTCAGTGA ATGTGAATGA ATGCGAATCT
    GCGCCATGTG AGAACGGAGG ATCTTGTGAA GATCTCGTCA ATGCTTTTCA ATGCTCATGT
    CCCCCAGGGT TCGCAGGTGC AGTGTGTGAG ACTGAGCTGG ACGAGTGTGA GAGTGACCCG
    TGTCAGAACC GCGGCCGCTG TGAGGACTCC ATTAATGCCT ACATATGCCA CTGTCCTCCT
    GCTGAGCCGG GACACCTGCC ATGGGGCGGT CCTGACTGCA GCGTCCAGCT GACCGGCTGT
    GTGGACCAAC CCTGCCAAAA CAACGCCACC TGCATCCCTT CAATACAGCA TCAGCAGCAC
    CAGCACACCT GCAGATGCCC ATCCGGCTTC CACGGCACCC ACTGCCAGAC CTCCACCACC
    TTCTCCATCA CCCGCGGAGG ATATTTACTT ATAGATCTTC ACCACAAAAA CAACAGGAGT
    CGCAGATATT TGGATTCACA TGTGCCGAGC GTCCAGCTAC GCTTCCGGAC GACGCTACAG
    GATGGAGTCA TCTTCTTCCG GGGAGGTCAA AATCACTTTT TTGTGCTTGA GTTAATTGCT
    GGAGAGCTGT ACTGCAGAGC TCAGTCTGGA GATTTGAAGC TCTTCGTGAA GCTGAACGGT
    GATTTCGGGG ATGGCTTCTG GCGGGGGGTT TCTGTGAGTG TGGATGAGGG GCTGACGGTG
    CTTTCAGAGG GTGAGATGGG TGAGGATGGA GGTCACAATC AGCTTCTGTC CTTCCAGCCG
    CAGGGTTTGG AGACACTGTA TGTGGGAGGA GTTCCTCAGG AGTGGATGAA CCTCACACTG
    AGCCGGACGG GGTTTGTGGG CTGTTTGGAG GATCTGGTTG TTGATTCTGA GCCGGTTCTT
    CCTCAGAGTT TGGTCCCGCT GCAGGAGGTT CAGATGGGCT GTGAGAGGAC TGAGTGGTGT
    CGTGTGAACC CCTGCTCTGA TCATGGACGC TGTGTGGATC TGTGGTCAAA CTACACGTGT
    GTCTGCCACA GGCCGTATTA TGGAGAGCAC TGTTCTGAAG AGCACACTTC ATGGACGTTC
    AGTCACGAGC GCAACAGAAG CTTCGCTGCG TTTCCCATCA TGCAGAATCA CAGCCGTGAC
    GTTACGGTGT CTTTATGGCT CAGATCACGC CACTTAAACG GCCTGGTCCT CCAGCTCCAG
    CACGACAATC AACCCTACCT CACTTTATTT CTGAAAAACG GCTCATTGTA TATATCAATA
    TACAGCTCCA TCAGAAGAGC CTCTGACTTC CTGACCAATG GGGAGAAGCT CTTCATGTCC
    ATTAAAACAG AGCCGGGAGT TATATTATTT AACCAAACGC AGCGGATTCC TATGATCTAC
    AGTGGTTTCG AGGTGCAAGT GGGAGATGTT GTGTATCTCG GTGGTCTTCC TGAAGGAGAG
    AACTCTGCGC TGTGGGGAGG ATATTTCAAA GGCTGTTTGC AGGACGTCCG AATCGACGAC
    ACACAACTGT TCATGTACTC CGGCAGCATC AGCCAGAAGC CACAACACGC TAATTACTTA
    CCCAGAAACT CATCCAATGT GCTGGAGGGC TGCGTGGGGG ACCAGACGTG CAAGATGAAG
    CCGTGTCTGA ACGGAGGGAA GTGTCAGGTC ATCTGGAATG ACTTTATGTG CCTCTGTCCG
    CTTAACTTCT CTGGAAAGAC GTGTGAAACT CGTGTCTGGT GTGTTAGCGA CCCCTGTGAC
    CCCAGGACTC GGTGTGTGGA TTTACCTGAC GGTTATGAGT GTTTAGCCAA CGCCACATTT
    GAAAACAACG CCCTGAAGTT CAGCGCAAAC GGCTCATTTT TTGAGCCTGT GATGAGAATT
    TCAATGGATC TGCGGACGCG AGAAGAAAAC GGGACCATTT TACGCTCCTC CAATGGAATC
    GAGTTCTTCT GCATGGGGCT ACTAAACTCC TCCATCATCG TGAAGTTTCG TGATAGTAAT
    AGTCTTGAAG TTCACGCTTT CATCAGCGAC GTCACCGTTT CTGATGGAGA ATGGCATAAA
    GTGGAGCTCT ATGCATCCAG CTCGAGAATA TCAGCGTTTC GTTGGCACAT GTCAATTGAT
    GGAAATCCTG CAGGATTCAG TCTCTCTCCT GCTGGAAATG TAGACTTTTT TAACTCCTCC
    ACTGTGATGC TGGCAGAAAA CTACACTGGG TGTTTGGGCG AAGTGCGCAT CGGTGGAGTT
    CACCTATCAT TTTTCGGACC TCTAAATGAA GAGGCGCCAC AAAAAACTCA ATTCATCCGA
    ATCGGTGGGA TGCTGGAGCC TCATTTAGGA TGCATTGGAT CTCCTTTATG TCTTTCCGAG
    CCCTGCCTGA ATAATGGCAC GTGCACGGAT CTCTTTAATC TTGCCGTGTG TAAATGTGCT
    CCAGGATGGA CCGGCGAGCG CTGCCAGGAG AATATTGACG AATGTGGAGA GCAGCCCTGT
    GTTCAGGGGT CCTGTCAGGA TCTGCTGGGG GATTATGAGT GCCGGTGTCC GGTTGGATTT
    GGTGGGAAGA ACTGTGATGT GGAGGTGGAC CAGTGCAAGG ATCATCAATG CGAGAATGGA
    GGGTCATGTG TGGCTACTGT CAGCGGATAC ACGTGTGTTT GTTTGCCTGG ACACACAGGG
    CCATACTGCC GATGGCGGTT TCCTCCTCGT GAGTGTGATG TGGATCTGCA GTGTGAGAAT
    GGAGGCGTTT GCAGTGAAGG ATCATGGGGA GCCAACTGTA CCTGCAGACC AGGATTCACT
    GGAGTCAGGT GTGAGCTGGA TATAGACGAG TGTGTGTCCG AGCCGTGTCT GAACGGAGGC
    TCATGTGTGA ACAGACACAA TCTCTACTTC TGTGAGTGTT TGCCGGACTT CAGTGGAGAA
    AACTGTCAGA TTACTAAGCA GCTTCAGCAG GAGCTATGGC CGTGGCTGCT GCTTCTGGTC
    CCGCTGGCGG GTCTCTCCGT ACTGCTGGCG GCTCTGGCTC TGCTCTGCCT GATCTTCAGC
    GCCCGGATGA AGCGCCGCTC GGAGGGAACA TACAGCCCCA GCCGGCAGGA GGCAGCAGGA
    GCACGAATGG AGATGGGCAG CGTGCTGAAG GTGCCTCCGG AAGAGAGACT CATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001038627.1
    CDS218..4594
    Translation

    Target ORF information:

    RefSeq Version NM_001045162.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio crumbs cell polarity complex component 2b (crb2b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001045162.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGATTTTG GACGGATGTG TTTACAGTAT AAAAGAGCGG TTCTGCTGTC ATTGATGTTC 
    AAGCTGGGTC TGTTCTGCAC CGTCTCATCA GAAGTGTGCC TGTCGTCCCC GTGCCAAAAC
    AACAGCACCT GTGTGCCCAC ATTGGACGGT TACCTGTGCA CCTGTTCCTC CAGCCCACCA
    ATATACACCG GCCCAAATTG TGAGCGTTTA CTGGACCCGT GTGACTCTGC AGGCTGTCCA
    TCCACATGCA TCGCCACTCC AGGAAGCCAA AACTACACCT GTCTGTGTCC TGATGGCTTC
    AGCGGCCCAA ACTGCACCCA AAACATCAAT GAATGTCACA GCAACCCCTG TTCGGGGGTC
    CGGAGCTTCT GTGTGGATGG AGTGAACGGG TATTCGTGTC ACTGTCCCAG CGGGTACAGC
    GGGGCAGACT GCGGGACCAG GGTGCGGGAC TGCTCAGATG ACCCGTGCTT TAATAATGCC
    ACCTGTGTCT GGGCTCCGGA CGGGTACGAG TGCCACTGTG CTGCAGGTTT TCGAGGTCGT
    AACTGTGAGG AGGACATCGA TGAGTGTCTG TCGCAGCCCT GCAGGAATGG GGCGATCTGT
    CAGGACGCTG TGGGGGTCTA CCAGTGCTAC TGCGTGCCTG GATTTCAGGG TTTTCACTGC
    GAGATTGATA TTAATGAGTG CGCATCGCAG CCCTGCGAGA ACAACGGGAC GTGTGTCAAT
    GGCAGAGACA GATACATCTG CGAGTGTCTC TTTGGGTTCA CAGGTCTCAA CTGTGAAGTG
    GACGTGGATG AATGTGAAGC GTCTCCCTGC CAGAACGGCG CCGTCTGCCA TGATCACGTG
    GGTCTGTACA CCTGTGAGTG TGTGCCGGGG TACGAGGGCA TCAACTGTGA GCTGGACATC
    GATGAGTGTG AGAGCTCACC CTGCCGCCAT GGAGGAGTCT GTCTGGATAT GGTCAACGGG
    TATGAGTGTG ACTGCAGTGG CACTGGATTC ATGGGCTTTT TCTGTGATAT TGACATCCCC
    GAGTGTTCTT CTGACCCCTG TCAACATGGC GCCACCTGCC TGGAGGGAGT AAACCACTAC
    ACCTGCATCT GCTGGACAGG TTATGAAGGA GTGAACTGTG AGCTGGATGT GGACGAGTGT
    GTTTCTCAGC CATGTGAGAA TGAAGGCCAG TGTTTCCAGC GCTCAGACTC CAGCCTCTAC
    AGAGTTCTGC CTGAACTGGA CACTGACTTC AGCTTCCAGA GCGCAGCCGG TTACCTGTGC
    CAGTGCGTAT CTGGCTTCAC AGGTGAAAAC TGCTCAGTGA ATGTGAATGA ATGCGAATCT
    GCGCCATGTG AGAACGGAGG ATCTTGTGAA GATCTCGTCA ATGCTTTTCA ATGCTCATGT
    CCCCCAGGGT TCGCAGGTGC AGTGTGTGAG ACTGAGCTGG ACGAGTGTGA GAGTGACCCG
    TGTCAGAACC GCGGCCGCTG TGAGGACTCC ATTAATGCCT ACATATGCCA CTGTCCTCCT
    GCTGAGCCGG GACACCTGCC ATGGGGCGGT CCTGACTGCA GCGTCCAGCT GACCGGCTGT
    GTGGACCAAC CCTGCCAAAA CAACGCCACC TGCATCCCTT CAATACAGCA TCAGCAGCAC
    CAGCACACCT GCAGATGCCC ATCCGGCTTC CACGGCACCC ACTGCCAGAC CTCCACCACC
    TTCTCCATCA CCCGCGGAGG ATATTTACTT ATAGATCTTC ACCACAAAAA CAACAGGAGT
    CGCAGATATT TGGATTCACA TGTGCCGAGC GTCCAGCTAC GCTTCCGGAC GACGCTACAG
    GATGGAGTCA TCTTCTTCCG GGGAGGTCAA AATCACTTTT TTGTGCTTGA GTTAATTGCT
    GGAGAGCTGT ACTGCAGAGC TCAGTCTGGA GATTTGAAGC TCTTCGTGAA GCTGAACGGT
    GATTTCGGGG ATGGCTTCTG GCGGGGGGTT TCTGTGAGTG TGGATGAGGG GCTGACGGTG
    CTTTCAGAGG GTGAGATGGG TGAGGATGGA GGTCACAATC AGCTTCTGTC CTTCCAGCCG
    CAGGGTTTGG AGACACTGTA TGTGGGAGGA GTTCCTCAGG AGTGGATGAA CCTCACACTG
    AGCCGGACGG GGTTTGTGGG CTGTTTGGAG GATCTGGTTG TTGATTCTGA GCCGGTTCTT
    CCTCAGAGTT TGGTCCCGCT GCAGGAGGTT CAGATGGGCT GTGAGAGGAC TGAGTGGTGT
    CGTGTGAACC CCTGCTCTGA TCATGGACGC TGTGTGGATC TGTGGTCAAA CTACACGTGT
    GTCTGCCACA GGCCGTATTA TGGAGAGCAC TGTTCTGAAG AGCACACTTC ATGGACGTTC
    AGTCACGAGC GCAACAGAAG CTTCGCTGCG TTTCCCATCA TGCAGAATCA CAGCCGTGAC
    GTTACGGTGT CTTTATGGCT CAGATCACGC CACTTAAACG GCCTGGTCCT CCAGCTCCAG
    CACGACAATC AACCCTACCT CACTTTATTT CTGAAAAACG GCTCATTGTA TATATCAATA
    TACAGCTCCA TCAGAAGAGC CTCTGACTTC CTGACCAATG GGGAGAAGCT CTTCATGTCC
    ATTAAAACAG AGCCGGGAGT TATATTATTT AACCAAACGC AGCGGATTCC TATGATCTAC
    AGTGGTTTCG AGGTGCAAGT GGGAGATGTT GTGTATCTCG GTGGTCTTCC TGAAGGAGAG
    AACTCTGCGC TGTGGGGAGG ATATTTCAAA GGCTGTTTGC AGGACGTCCG AATCGACGAC
    ACACAACTGT TCATGTACTC CGGCAGCATC AGCCAGAAGC CACAACACGC TAATTACTTA
    CCCAGAAACT CATCCAATGT GCTGGAGGGC TGCGTGGGGG ACCAGACGTG CAAGATGAAG
    CCGTGTCTGA ACGGAGGGAA GTGTCAGGTC ATCTGGAATG ACTTTATGTG CCTCTGTCCG
    CTTAACTTCT CTGGAAAGAC GTGTGAAACT CGTGTCTGGT GTGTTAGCGA CCCCTGTGAC
    CCCAGGACTC GGTGTGTGGA TTTACCTGAC GGTTATGAGT GTTTAGCCAA CGCCACATTT
    GAAAACAACG CCCTGAAGTT CAGCGCAAAC GGCTCATTTT TTGAGCCTGT GATGAGAATT
    TCAATGGATC TGCGGACGCG AGAAGAAAAC GGGACCATTT TACGCTCCTC CAATGGAATC
    GAGTTCTTCT GCATGGGGCT ACTAAACTCC TCCATCATCG TGAAGTTTCG TGATAGTAAT
    AGTCTTGAAG TTCACGCTTT CATCAGCGAC GTCACCGTTT CTGATGGAGA ATGGCATAAA
    GTGGAGCTCT ATGCATCCAG CTCGAGAATA TCAGCGTTTC GTTGGCACAT GTCAATTGAT
    GGAAATCCTG CAGGATTCAG TCTCTCTCCT GCTGGAAATG TAGACTTTTT TAACTCCTCC
    ACTGTGATGC TGGCAGAAAA CTACACTGGG TGTTTGGGCG AAGTGCGCAT CGGTGGAGTT
    CACCTATCAT TTTTCGGACC TCTAAATGAA GAGGCGCCAC AAAAAACTCA ATTCATCCGA
    ATCGGTGGGA TGCTGGAGCC TCATTTAGGA TGCATTGGAT CTCCTTTATG TCTTTCCGAG
    CCCTGCCTGA ATAATGGCAC GTGCACGGAT CTCTTTAATC TTGCCGTGTG TAAATGTGCT
    CCAGGATGGA CCGGCGAGCG CTGCCAGGAG AATATTGACG AATGTGGAGA GCAGCCCTGT
    GTTCAGGGGT CCTGTCAGGA TCTGCTGGGG GATTATGAGT GCCGGTGTCC GGTTGGATTT
    GGTGGGAAGA ACTGTGATGT GGAGGTGGAC CAGTGCAAGG ATCATCAATG CGAGAATGGA
    GGGTCATGTG TGGCTACTGT CAGCGGATAC ACGTGTGTTT GTTTGCCTGG ACACACAGGG
    CCATACTGCC GATGGCGGTT TCCTCCTCGT GAGTGTGATG TGGATCTGCA GTGTGAGAAT
    GGAGGCGTTT GCAGTGAAGG ATCATGGGGA GCCAACTGTA CCTGCAGACC AGGATTCACT
    GGAGTCAGGT GTGAGCTGGA TATAGACGAG TGTGTGTCCG AGCCGTGTCT GAACGGAGGC
    TCATGTGTGA ACAGACACAA TCTCTACTTC TGTGAGTGTT TGCCGGACTT CAGTGGAGAA
    AACTGTCAGA TTACTAAGCA GCTTCAGCAG GAGCTATGGC CGTGGCTGCT GCTTCTGGTC
    CCGCTGGCGG GTCTCTCCGT ACTGCTGGCG GCTCTGGCTC TGCTCTGCCT GATCTTCAGC
    GCCCGGATGA AGCGCCGCTC GGAGGGAACA TACAGCCCCA GCCGGCAGGA GGCAGCAGGA
    GCACGAATGG AGATGGGCAG CGTGCTGAAG GTGCCTCCGG AAGAGAGACT CATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45443
    Clone ID Related Accession (Same CDS sequence) XM_017357360.1 , XM_017357360.2
    Accession Version XM_017357360.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4377bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product crumbs family member 2b isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGATTTTG GACGGATGTG TTTACAGTAT AAAAGAGCAG TTCTGCTGTC ATTGATGTTC 
    AAGCTGGGTC TGTTCTGCAC AGTCTCATCA GAAGTGTGCC TGTCGTCCCC GTGCCAAAAC
    AACAGCACCT GTGTGCCCAC ATTGGACGGT TACCTGTGCA CCTGTTCCTC CAGCCCACCA
    ATATACACCG GCCCAAATTG TGAGCGTTTA CTGGACCCGT GTGACTCTGC AGGCTGTCCA
    TCCACATGCA TCGCCACTCC AGGAAGCCAA AACTACACCT GTCTGTGTCC TGATGGCTTC
    AGCGGCCCAA ACTGCACCCA AAACATCAAT GAATGTCACA GCAACCCCTG TTCGGGAGTC
    CGGAGCTTCT GTGTGGATGG AGTGAACGGG TATTCGTGTC ACTGTCCCAG CGGGTACAGC
    GGGGCAGACT GCGGGACCAG GGTGCGGGAC TGCTCAGATG ACCCGTGCTT TAATAATGCC
    ACCTGTGTCT GGGCTCCGGA CGGGTACGAG TGCCACTGTG CTGCAGGTTT TCGAGGTCGT
    AACTGTGAGG AGGACATCGA TGAGTGTCTG TCGCAGCCCT GCAGGAATGG GGCGATCTGT
    CAGGACGCTG TGGGGGTCTA CCAGTGCTAC TGCGTGCCTG GATTTCAGGG TTTTCACTGC
    GAGATTGATA TTAATGAGTG TGCATCGCAG CCCTGCGAGA ACAACGGGAC GTGTGTCAAT
    GGCAGAGACA GATACATCTG CGAATGTCTC TTTGGGTTCA CAGGTCTCAA CTGTGAAGTG
    GACGTGGATG AATGTGAAGC GTCTCCCTGC CAGAACGGCG CCGTCTGCCA TGATCACGTG
    GGTCTGTACA CCTGTGAGTG TGTGCCGGGG TACGAGGGCA TCAACTGTGA GCTGGACATC
    GATGAGTGTG AGAGCTCACC CTGCCGCCAT GGAGGAGTCT GTCTGGATAT GGTCAACGGG
    TATGAGTGTG ACTGCAGTGG CACTGGATTC ATGGGCTTTT TCTGTGATAT TGACATCCCC
    GAGTGTTCTT CTGACCCCTG TCAACATGGC GCCACCTGCC TGGAGGGTGT AAACCACTAC
    ACCTGCATCT GCTGGACAGG TTATGAAGGA GTGAACTGTG AGCTGGATGT GGACGAGTGT
    GTTTCTCAGC CATGTGAGAA TGAAGGCCAG TGTTTCCAGC GCTCAGACTC CAGCCTCTAC
    AGAGTTCTGC CTGAACTGGA CACTGACTTC AGCTTCCAGA GCGCAGCCGG TTACCTGTGC
    CAGTGCGTAT CTGGCTTCAC AGGTGAAAAC TGCTCAGTGA ATGTGAATGA ATGCGAATCT
    GCGCCATGTG AGAACGGAGG ATCTTGTGAA GATCTCGTCA ATGCTTTTCA ATGCTCATGT
    CCCCCAGGGT TCGCAGGTGC AGTGTGTGAG ACTGAGCTGG ACGAGTGTGA GAGTGATCCG
    TGTCAGAACG GCGGCCGCTG TGAGGACTCC ATTAATGCCT ACACATGCCA CTGTCCTCCT
    GCTGAGCCGG GACACCTGCC ATGGGGCGGT CCTGACTGCA GCGTCCAGCT GACCGGCTGT
    GTGGACCAAC CCTGCCAAAA CAACGCCACC TGCATCCCTT CAATACAGCA TCAGCAGCAC
    CAGCACACCT GCAGATGCCC ATCCGGCTTC CACGGCACCC ACTGCCAGAC CTCCACCACC
    TTCTCCATCA CCCGCGGAGG ATATTTACTT ATAGATCTTC ATCACAAAAA CAACAGGAGT
    CGCAGATATT TGGATTCACA TGTGCCGAGC GTCCAGCTAC GCTTCCGGAC GACGCTACAG
    GATGGAGTCA TCTTCTTCCG GGGAGGTCAA AATCACTTTT TTGTGCTTGA GTTAATTGCT
    GGAGAGCTGT GCTGCAGAGC TCAGTCTGGA GATTTGAAGC TCTTCGTGAA GCTGAGCGGT
    GATTTCGGGG ATGGCTTCTG GCGAGGGGTT TCTGTGAGTG TGGATGAGGG GCTGACGGTG
    CTTTCAGAGG GTGAGATGGG TGAGGATGGA GGTCACAATC AGCTTCTGTC CTTCCAGCCG
    CAGGGTTTGG AGACACTGTA TGTGGGAGGA GTTCCTCAGG AGTGGATGAA CCTCACACTG
    AGCCGGACGG GGTTTGTGGG CTGTTTGGAG GATCTGGTTG TTGATTCTGA GCCGGTTCTT
    CCTCAGAGTT TGGTCCCGCT GCAGGAGGTT CAGATGGGCT GTGAGAGAAC TGAGTGGTGT
    CGTGTGAACC CCTGCTCTGA TCATGGACGC TGTGTGGATC TGTGGTCAAA CTACACGTGT
    GTCTGCCACA GGCCGTATTA TGGAGAGCAC TGTTCTGAAG AGCACACTTC ATGGACGTTC
    AGTCACGAGC GCAACAGAAG CTTCGCTGCG TTTCCCATCA TGCAGAATCA CAGCCGTGAC
    GTTACGGTGT CTTTATGGCT CAGATCACGC CACTTAAACG GCCTGGTCCT CCAGCTCCAG
    CACGACAATC AACCCTACCT CACTTTATTT CTGAAAAACG GCTCACTGTA TATATCAATA
    TACAGCTCCA TCAGAAGAGC CTCTGACTTC CTGACCAATG GGGAGAAGCT CTTCATGTCC
    ATTAAAACAG AGCCGGGAGT TATATTATTT AACCAAACGC AGCGGATTCC TATGATCTAC
    AGTGGCTTCG AGGTGCAAGT GGGAGATGTT GTGTATCTCG GTGGTCTTCC TGAAGGAGAG
    AACTCTGCGC TGTGGGGAGG ATATTTCAAA GGCTGTTTGC AGGACGTCCG AATCGACGAC
    ACACAACTGT TCATGTACTC CGGCAGCATC AGCCAGAAGC CACAACACGC TAATTACTTA
    CCCAGAAACT CATCCAATGT GCTGGAGGGC TGCGTGGGGG ACCAGACGTG CAAGATGAAG
    CCGTGTCTGA ACGGAGGGAA GTGTCAGGTC ATCTGGAATG ACTTTATGTG CCTCTGTCCG
    CTTAACTTCT CTGGAAAGAC GTGTGAAACT CGTGTCTGGT GTGTTAGCGA CCCCTGTGAC
    CCCAGGACTC GGTGTGTGGA TTTACCTGAC GGTTATGAGT GTTTAGCCAA CGCCACATTT
    GAAAACAACG CCCTGAAGTT CAGCGCAAAC GGCTCATTTT TTGAGCCTGT GATGAGAATT
    TCAATGGATC TGCGGACGCG AGAAGAAAAC GGGACCATTT TACGCTCCTC CAATGGAATC
    GAGTTCTTCT GCATGGGGCT ACTAAACTCC TCCATCATCG TGAAGTTTCG TGATAGTAAT
    AGTCTTGAAG TTCACGCTTT CATCAGCGAC GTCACCGTTT CTGATGGAGA ATGGCATAAA
    GTGGAGCTCT ATGCATCCAG CTCGAGAATA TCAGCGTTTC GTTGGCACAT GTCAATTGAT
    GGAAATCCTG CAGGATTCAG TCTCTCTCCT GCTGGAAATG TAGACTTTTT TAACTCCTCC
    ACTGTGATGC TGGCAGAAAA CTACACTGGG TGTTTGGGCG AAGTGCGCAT CGGTGGAGTT
    CACCTATCAT TTTTCGGACC TCTAAATGAA GAGGCGCCAC AAAAAACTCA ATTCATCCGA
    ATCGGTGGGA TGCTGGAGCC TCATTTAGGA TGCATTGGAT CTCCTTTATG TCTTTCCGAG
    CCCTGCCTGA ATAATGGCAC GTGCACGGAT CTCTTTAATC TTGCCGTGTG TAAATGTGCT
    CCAGGATGGA CCGGCGAGCG CTGCCAGGAG AATATTGACG AATGTGGAGA GCAGCCCTGT
    GTTCAGGGGT CCTGTCAGGA TCTGCTGGGG GATTATGAGT GCCGGTGTCC GGTTGGATTT
    GGTGGGAAGA ACTGTGATGT GGAGGTGGAC CAGTGCAAGG ATCATCAATG CGAGAATGGA
    GGGTCATGTG TGGCTACTGT CAGCGGATAC ACGTGTGTTT GTTTGCCTGG ACACACAGGG
    CCATACTGCC GATGGCGGTT TCCTCCTCGT GAGTGTGATG TGGATCTGCA GTGTGAGAAT
    GGAGGCGTTT GCAGTGAAGG ATCATGGGGA GCCAACTGTA CCTGCAGACC AGGATTCACT
    GGAGTCAGGT GTGAGCTGGA TATAGACGAG TGTGTGTCCG AGCCGTGTCT GAACGGAGGC
    TCATGTGTGA ACAGACACAA TCTCTACTTC TGTGAGTGTT TGCCGGACTT CAGTGGAGAA
    AACTGTCAGA TTACTAAGCA GCTTCAGCAG GAGCTATGGC CGTGGCTGCT GCTCCTGGTC
    CCGCTGGCGG GTCTCTCCGT ACTGCTGGCG GCTCTGGCTC TGCTCTGCCT GATCTTCAGC
    GCCCGGAAGA AGCGCCGCTC GGAGGGAACA TACAGCCCCA GCCGGCAGGA GGCAGCAGGA
    GCACGAATGG AGATGGGCAG CGTGCTGAAG GTGCCTCCGG AAGAGAGACT CATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212849.1
    CDS220..4596
    Translation

    Target ORF information:

    RefSeq Version XM_017357360.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio crumbs family member 2b (crb2b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017357360.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGATTTTG GACGGATGTG TTTACAGTAT AAAAGAGCAG TTCTGCTGTC ATTGATGTTC 
    AAGCTGGGTC TGTTCTGCAC AGTCTCATCA GAAGTGTGCC TGTCGTCCCC GTGCCAAAAC
    AACAGCACCT GTGTGCCCAC ATTGGACGGT TACCTGTGCA CCTGTTCCTC CAGCCCACCA
    ATATACACCG GCCCAAATTG TGAGCGTTTA CTGGACCCGT GTGACTCTGC AGGCTGTCCA
    TCCACATGCA TCGCCACTCC AGGAAGCCAA AACTACACCT GTCTGTGTCC TGATGGCTTC
    AGCGGCCCAA ACTGCACCCA AAACATCAAT GAATGTCACA GCAACCCCTG TTCGGGAGTC
    CGGAGCTTCT GTGTGGATGG AGTGAACGGG TATTCGTGTC ACTGTCCCAG CGGGTACAGC
    GGGGCAGACT GCGGGACCAG GGTGCGGGAC TGCTCAGATG ACCCGTGCTT TAATAATGCC
    ACCTGTGTCT GGGCTCCGGA CGGGTACGAG TGCCACTGTG CTGCAGGTTT TCGAGGTCGT
    AACTGTGAGG AGGACATCGA TGAGTGTCTG TCGCAGCCCT GCAGGAATGG GGCGATCTGT
    CAGGACGCTG TGGGGGTCTA CCAGTGCTAC TGCGTGCCTG GATTTCAGGG TTTTCACTGC
    GAGATTGATA TTAATGAGTG TGCATCGCAG CCCTGCGAGA ACAACGGGAC GTGTGTCAAT
    GGCAGAGACA GATACATCTG CGAATGTCTC TTTGGGTTCA CAGGTCTCAA CTGTGAAGTG
    GACGTGGATG AATGTGAAGC GTCTCCCTGC CAGAACGGCG CCGTCTGCCA TGATCACGTG
    GGTCTGTACA CCTGTGAGTG TGTGCCGGGG TACGAGGGCA TCAACTGTGA GCTGGACATC
    GATGAGTGTG AGAGCTCACC CTGCCGCCAT GGAGGAGTCT GTCTGGATAT GGTCAACGGG
    TATGAGTGTG ACTGCAGTGG CACTGGATTC ATGGGCTTTT TCTGTGATAT TGACATCCCC
    GAGTGTTCTT CTGACCCCTG TCAACATGGC GCCACCTGCC TGGAGGGTGT AAACCACTAC
    ACCTGCATCT GCTGGACAGG TTATGAAGGA GTGAACTGTG AGCTGGATGT GGACGAGTGT
    GTTTCTCAGC CATGTGAGAA TGAAGGCCAG TGTTTCCAGC GCTCAGACTC CAGCCTCTAC
    AGAGTTCTGC CTGAACTGGA CACTGACTTC AGCTTCCAGA GCGCAGCCGG TTACCTGTGC
    CAGTGCGTAT CTGGCTTCAC AGGTGAAAAC TGCTCAGTGA ATGTGAATGA ATGCGAATCT
    GCGCCATGTG AGAACGGAGG ATCTTGTGAA GATCTCGTCA ATGCTTTTCA ATGCTCATGT
    CCCCCAGGGT TCGCAGGTGC AGTGTGTGAG ACTGAGCTGG ACGAGTGTGA GAGTGATCCG
    TGTCAGAACG GCGGCCGCTG TGAGGACTCC ATTAATGCCT ACACATGCCA CTGTCCTCCT
    GCTGAGCCGG GACACCTGCC ATGGGGCGGT CCTGACTGCA GCGTCCAGCT GACCGGCTGT
    GTGGACCAAC CCTGCCAAAA CAACGCCACC TGCATCCCTT CAATACAGCA TCAGCAGCAC
    CAGCACACCT GCAGATGCCC ATCCGGCTTC CACGGCACCC ACTGCCAGAC CTCCACCACC
    TTCTCCATCA CCCGCGGAGG ATATTTACTT ATAGATCTTC ATCACAAAAA CAACAGGAGT
    CGCAGATATT TGGATTCACA TGTGCCGAGC GTCCAGCTAC GCTTCCGGAC GACGCTACAG
    GATGGAGTCA TCTTCTTCCG GGGAGGTCAA AATCACTTTT TTGTGCTTGA GTTAATTGCT
    GGAGAGCTGT GCTGCAGAGC TCAGTCTGGA GATTTGAAGC TCTTCGTGAA GCTGAGCGGT
    GATTTCGGGG ATGGCTTCTG GCGAGGGGTT TCTGTGAGTG TGGATGAGGG GCTGACGGTG
    CTTTCAGAGG GTGAGATGGG TGAGGATGGA GGTCACAATC AGCTTCTGTC CTTCCAGCCG
    CAGGGTTTGG AGACACTGTA TGTGGGAGGA GTTCCTCAGG AGTGGATGAA CCTCACACTG
    AGCCGGACGG GGTTTGTGGG CTGTTTGGAG GATCTGGTTG TTGATTCTGA GCCGGTTCTT
    CCTCAGAGTT TGGTCCCGCT GCAGGAGGTT CAGATGGGCT GTGAGAGAAC TGAGTGGTGT
    CGTGTGAACC CCTGCTCTGA TCATGGACGC TGTGTGGATC TGTGGTCAAA CTACACGTGT
    GTCTGCCACA GGCCGTATTA TGGAGAGCAC TGTTCTGAAG AGCACACTTC ATGGACGTTC
    AGTCACGAGC GCAACAGAAG CTTCGCTGCG TTTCCCATCA TGCAGAATCA CAGCCGTGAC
    GTTACGGTGT CTTTATGGCT CAGATCACGC CACTTAAACG GCCTGGTCCT CCAGCTCCAG
    CACGACAATC AACCCTACCT CACTTTATTT CTGAAAAACG GCTCACTGTA TATATCAATA
    TACAGCTCCA TCAGAAGAGC CTCTGACTTC CTGACCAATG GGGAGAAGCT CTTCATGTCC
    ATTAAAACAG AGCCGGGAGT TATATTATTT AACCAAACGC AGCGGATTCC TATGATCTAC
    AGTGGCTTCG AGGTGCAAGT GGGAGATGTT GTGTATCTCG GTGGTCTTCC TGAAGGAGAG
    AACTCTGCGC TGTGGGGAGG ATATTTCAAA GGCTGTTTGC AGGACGTCCG AATCGACGAC
    ACACAACTGT TCATGTACTC CGGCAGCATC AGCCAGAAGC CACAACACGC TAATTACTTA
    CCCAGAAACT CATCCAATGT GCTGGAGGGC TGCGTGGGGG ACCAGACGTG CAAGATGAAG
    CCGTGTCTGA ACGGAGGGAA GTGTCAGGTC ATCTGGAATG ACTTTATGTG CCTCTGTCCG
    CTTAACTTCT CTGGAAAGAC GTGTGAAACT CGTGTCTGGT GTGTTAGCGA CCCCTGTGAC
    CCCAGGACTC GGTGTGTGGA TTTACCTGAC GGTTATGAGT GTTTAGCCAA CGCCACATTT
    GAAAACAACG CCCTGAAGTT CAGCGCAAAC GGCTCATTTT TTGAGCCTGT GATGAGAATT
    TCAATGGATC TGCGGACGCG AGAAGAAAAC GGGACCATTT TACGCTCCTC CAATGGAATC
    GAGTTCTTCT GCATGGGGCT ACTAAACTCC TCCATCATCG TGAAGTTTCG TGATAGTAAT
    AGTCTTGAAG TTCACGCTTT CATCAGCGAC GTCACCGTTT CTGATGGAGA ATGGCATAAA
    GTGGAGCTCT ATGCATCCAG CTCGAGAATA TCAGCGTTTC GTTGGCACAT GTCAATTGAT
    GGAAATCCTG CAGGATTCAG TCTCTCTCCT GCTGGAAATG TAGACTTTTT TAACTCCTCC
    ACTGTGATGC TGGCAGAAAA CTACACTGGG TGTTTGGGCG AAGTGCGCAT CGGTGGAGTT
    CACCTATCAT TTTTCGGACC TCTAAATGAA GAGGCGCCAC AAAAAACTCA ATTCATCCGA
    ATCGGTGGGA TGCTGGAGCC TCATTTAGGA TGCATTGGAT CTCCTTTATG TCTTTCCGAG
    CCCTGCCTGA ATAATGGCAC GTGCACGGAT CTCTTTAATC TTGCCGTGTG TAAATGTGCT
    CCAGGATGGA CCGGCGAGCG CTGCCAGGAG AATATTGACG AATGTGGAGA GCAGCCCTGT
    GTTCAGGGGT CCTGTCAGGA TCTGCTGGGG GATTATGAGT GCCGGTGTCC GGTTGGATTT
    GGTGGGAAGA ACTGTGATGT GGAGGTGGAC CAGTGCAAGG ATCATCAATG CGAGAATGGA
    GGGTCATGTG TGGCTACTGT CAGCGGATAC ACGTGTGTTT GTTTGCCTGG ACACACAGGG
    CCATACTGCC GATGGCGGTT TCCTCCTCGT GAGTGTGATG TGGATCTGCA GTGTGAGAAT
    GGAGGCGTTT GCAGTGAAGG ATCATGGGGA GCCAACTGTA CCTGCAGACC AGGATTCACT
    GGAGTCAGGT GTGAGCTGGA TATAGACGAG TGTGTGTCCG AGCCGTGTCT GAACGGAGGC
    TCATGTGTGA ACAGACACAA TCTCTACTTC TGTGAGTGTT TGCCGGACTT CAGTGGAGAA
    AACTGTCAGA TTACTAAGCA GCTTCAGCAG GAGCTATGGC CGTGGCTGCT GCTCCTGGTC
    CCGCTGGCGG GTCTCTCCGT ACTGCTGGCG GCTCTGGCTC TGCTCTGCCT GATCTTCAGC
    GCCCGGAAGA AGCGCCGCTC GGAGGGAACA TACAGCCCCA GCCGGCAGGA GGCAGCAGGA
    GCACGAATGG AGATGGGCAG CGTGCTGAAG GTGCCTCCGG AAGAGAGACT CATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa45443
    Clone ID Related Accession (Same CDS sequence) XM_017357360.1 , XM_017357360.2
    Accession Version XM_017357360.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4377bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product crumbs family member 2b isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357360.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGATTTTG GACGGATGTG TTTACAGTAT AAAAGAGCAG TTCTGCTGTC ATTGATGTTC 
    AAGCTGGGTC TGTTCTGCAC AGTCTCATCA GAAGTGTGCC TGTCGTCCCC GTGCCAAAAC
    AACAGCACCT GTGTGCCCAC ATTGGACGGT TACCTGTGCA CCTGTTCCTC CAGCCCACCA
    ATATACACCG GCCCAAATTG TGAGCGTTTA CTGGACCCGT GTGACTCTGC AGGCTGTCCA
    TCCACATGCA TCGCCACTCC AGGAAGCCAA AACTACACCT GTCTGTGTCC TGATGGCTTC
    AGCGGCCCAA ACTGCACCCA AAACATCAAT GAATGTCACA GCAACCCCTG TTCGGGAGTC
    CGGAGCTTCT GTGTGGATGG AGTGAACGGG TATTCGTGTC ACTGTCCCAG CGGGTACAGC
    GGGGCAGACT GCGGGACCAG GGTGCGGGAC TGCTCAGATG ACCCGTGCTT TAATAATGCC
    ACCTGTGTCT GGGCTCCGGA CGGGTACGAG TGCCACTGTG CTGCAGGTTT TCGAGGTCGT
    AACTGTGAGG AGGACATCGA TGAGTGTCTG TCGCAGCCCT GCAGGAATGG GGCGATCTGT
    CAGGACGCTG TGGGGGTCTA CCAGTGCTAC TGCGTGCCTG GATTTCAGGG TTTTCACTGC
    GAGATTGATA TTAATGAGTG TGCATCGCAG CCCTGCGAGA ACAACGGGAC GTGTGTCAAT
    GGCAGAGACA GATACATCTG CGAATGTCTC TTTGGGTTCA CAGGTCTCAA CTGTGAAGTG
    GACGTGGATG AATGTGAAGC GTCTCCCTGC CAGAACGGCG CCGTCTGCCA TGATCACGTG
    GGTCTGTACA CCTGTGAGTG TGTGCCGGGG TACGAGGGCA TCAACTGTGA GCTGGACATC
    GATGAGTGTG AGAGCTCACC CTGCCGCCAT GGAGGAGTCT GTCTGGATAT GGTCAACGGG
    TATGAGTGTG ACTGCAGTGG CACTGGATTC ATGGGCTTTT TCTGTGATAT TGACATCCCC
    GAGTGTTCTT CTGACCCCTG TCAACATGGC GCCACCTGCC TGGAGGGTGT AAACCACTAC
    ACCTGCATCT GCTGGACAGG TTATGAAGGA GTGAACTGTG AGCTGGATGT GGACGAGTGT
    GTTTCTCAGC CATGTGAGAA TGAAGGCCAG TGTTTCCAGC GCTCAGACTC CAGCCTCTAC
    AGAGTTCTGC CTGAACTGGA CACTGACTTC AGCTTCCAGA GCGCAGCCGG TTACCTGTGC
    CAGTGCGTAT CTGGCTTCAC AGGTGAAAAC TGCTCAGTGA ATGTGAATGA ATGCGAATCT
    GCGCCATGTG AGAACGGAGG ATCTTGTGAA GATCTCGTCA ATGCTTTTCA ATGCTCATGT
    CCCCCAGGGT TCGCAGGTGC AGTGTGTGAG ACTGAGCTGG ACGAGTGTGA GAGTGATCCG
    TGTCAGAACG GCGGCCGCTG TGAGGACTCC ATTAATGCCT ACACATGCCA CTGTCCTCCT
    GCTGAGCCGG GACACCTGCC ATGGGGCGGT CCTGACTGCA GCGTCCAGCT GACCGGCTGT
    GTGGACCAAC CCTGCCAAAA CAACGCCACC TGCATCCCTT CAATACAGCA TCAGCAGCAC
    CAGCACACCT GCAGATGCCC ATCCGGCTTC CACGGCACCC ACTGCCAGAC CTCCACCACC
    TTCTCCATCA CCCGCGGAGG ATATTTACTT ATAGATCTTC ATCACAAAAA CAACAGGAGT
    CGCAGATATT TGGATTCACA TGTGCCGAGC GTCCAGCTAC GCTTCCGGAC GACGCTACAG
    GATGGAGTCA TCTTCTTCCG GGGAGGTCAA AATCACTTTT TTGTGCTTGA GTTAATTGCT
    GGAGAGCTGT GCTGCAGAGC TCAGTCTGGA GATTTGAAGC TCTTCGTGAA GCTGAGCGGT
    GATTTCGGGG ATGGCTTCTG GCGAGGGGTT TCTGTGAGTG TGGATGAGGG GCTGACGGTG
    CTTTCAGAGG GTGAGATGGG TGAGGATGGA GGTCACAATC AGCTTCTGTC CTTCCAGCCG
    CAGGGTTTGG AGACACTGTA TGTGGGAGGA GTTCCTCAGG AGTGGATGAA CCTCACACTG
    AGCCGGACGG GGTTTGTGGG CTGTTTGGAG GATCTGGTTG TTGATTCTGA GCCGGTTCTT
    CCTCAGAGTT TGGTCCCGCT GCAGGAGGTT CAGATGGGCT GTGAGAGAAC TGAGTGGTGT
    CGTGTGAACC CCTGCTCTGA TCATGGACGC TGTGTGGATC TGTGGTCAAA CTACACGTGT
    GTCTGCCACA GGCCGTATTA TGGAGAGCAC TGTTCTGAAG AGCACACTTC ATGGACGTTC
    AGTCACGAGC GCAACAGAAG CTTCGCTGCG TTTCCCATCA TGCAGAATCA CAGCCGTGAC
    GTTACGGTGT CTTTATGGCT CAGATCACGC CACTTAAACG GCCTGGTCCT CCAGCTCCAG
    CACGACAATC AACCCTACCT CACTTTATTT CTGAAAAACG GCTCACTGTA TATATCAATA
    TACAGCTCCA TCAGAAGAGC CTCTGACTTC CTGACCAATG GGGAGAAGCT CTTCATGTCC
    ATTAAAACAG AGCCGGGAGT TATATTATTT AACCAAACGC AGCGGATTCC TATGATCTAC
    AGTGGCTTCG AGGTGCAAGT GGGAGATGTT GTGTATCTCG GTGGTCTTCC TGAAGGAGAG
    AACTCTGCGC TGTGGGGAGG ATATTTCAAA GGCTGTTTGC AGGACGTCCG AATCGACGAC
    ACACAACTGT TCATGTACTC CGGCAGCATC AGCCAGAAGC CACAACACGC TAATTACTTA
    CCCAGAAACT CATCCAATGT GCTGGAGGGC TGCGTGGGGG ACCAGACGTG CAAGATGAAG
    CCGTGTCTGA ACGGAGGGAA GTGTCAGGTC ATCTGGAATG ACTTTATGTG CCTCTGTCCG
    CTTAACTTCT CTGGAAAGAC GTGTGAAACT CGTGTCTGGT GTGTTAGCGA CCCCTGTGAC
    CCCAGGACTC GGTGTGTGGA TTTACCTGAC GGTTATGAGT GTTTAGCCAA CGCCACATTT
    GAAAACAACG CCCTGAAGTT CAGCGCAAAC GGCTCATTTT TTGAGCCTGT GATGAGAATT
    TCAATGGATC TGCGGACGCG AGAAGAAAAC GGGACCATTT TACGCTCCTC CAATGGAATC
    GAGTTCTTCT GCATGGGGCT ACTAAACTCC TCCATCATCG TGAAGTTTCG TGATAGTAAT
    AGTCTTGAAG TTCACGCTTT CATCAGCGAC GTCACCGTTT CTGATGGAGA ATGGCATAAA
    GTGGAGCTCT ATGCATCCAG CTCGAGAATA TCAGCGTTTC GTTGGCACAT GTCAATTGAT
    GGAAATCCTG CAGGATTCAG TCTCTCTCCT GCTGGAAATG TAGACTTTTT TAACTCCTCC
    ACTGTGATGC TGGCAGAAAA CTACACTGGG TGTTTGGGCG AAGTGCGCAT CGGTGGAGTT
    CACCTATCAT TTTTCGGACC TCTAAATGAA GAGGCGCCAC AAAAAACTCA ATTCATCCGA
    ATCGGTGGGA TGCTGGAGCC TCATTTAGGA TGCATTGGAT CTCCTTTATG TCTTTCCGAG
    CCCTGCCTGA ATAATGGCAC GTGCACGGAT CTCTTTAATC TTGCCGTGTG TAAATGTGCT
    CCAGGATGGA CCGGCGAGCG CTGCCAGGAG AATATTGACG AATGTGGAGA GCAGCCCTGT
    GTTCAGGGGT CCTGTCAGGA TCTGCTGGGG GATTATGAGT GCCGGTGTCC GGTTGGATTT
    GGTGGGAAGA ACTGTGATGT GGAGGTGGAC CAGTGCAAGG ATCATCAATG CGAGAATGGA
    GGGTCATGTG TGGCTACTGT CAGCGGATAC ACGTGTGTTT GTTTGCCTGG ACACACAGGG
    CCATACTGCC GATGGCGGTT TCCTCCTCGT GAGTGTGATG TGGATCTGCA GTGTGAGAAT
    GGAGGCGTTT GCAGTGAAGG ATCATGGGGA GCCAACTGTA CCTGCAGACC AGGATTCACT
    GGAGTCAGGT GTGAGCTGGA TATAGACGAG TGTGTGTCCG AGCCGTGTCT GAACGGAGGC
    TCATGTGTGA ACAGACACAA TCTCTACTTC TGTGAGTGTT TGCCGGACTT CAGTGGAGAA
    AACTGTCAGA TTACTAAGCA GCTTCAGCAG GAGCTATGGC CGTGGCTGCT GCTCCTGGTC
    CCGCTGGCGG GTCTCTCCGT ACTGCTGGCG GCTCTGGCTC TGCTCTGCCT GATCTTCAGC
    GCCCGGAAGA AGCGCCGCTC GGAGGGAACA TACAGCCCCA GCCGGCAGGA GGCAGCAGGA
    GCACGAATGG AGATGGGCAG CGTGCTGAAG GTGCCTCCGG AAGAGAGACT CATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212849.1
    CDS260..4636
    Translation

    Target ORF information:

    RefSeq Version XM_017357360.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio crumbs family member 2b (crb2b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017357360.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGATTTTG GACGGATGTG TTTACAGTAT AAAAGAGCAG TTCTGCTGTC ATTGATGTTC 
    AAGCTGGGTC TGTTCTGCAC AGTCTCATCA GAAGTGTGCC TGTCGTCCCC GTGCCAAAAC
    AACAGCACCT GTGTGCCCAC ATTGGACGGT TACCTGTGCA CCTGTTCCTC CAGCCCACCA
    ATATACACCG GCCCAAATTG TGAGCGTTTA CTGGACCCGT GTGACTCTGC AGGCTGTCCA
    TCCACATGCA TCGCCACTCC AGGAAGCCAA AACTACACCT GTCTGTGTCC TGATGGCTTC
    AGCGGCCCAA ACTGCACCCA AAACATCAAT GAATGTCACA GCAACCCCTG TTCGGGAGTC
    CGGAGCTTCT GTGTGGATGG AGTGAACGGG TATTCGTGTC ACTGTCCCAG CGGGTACAGC
    GGGGCAGACT GCGGGACCAG GGTGCGGGAC TGCTCAGATG ACCCGTGCTT TAATAATGCC
    ACCTGTGTCT GGGCTCCGGA CGGGTACGAG TGCCACTGTG CTGCAGGTTT TCGAGGTCGT
    AACTGTGAGG AGGACATCGA TGAGTGTCTG TCGCAGCCCT GCAGGAATGG GGCGATCTGT
    CAGGACGCTG TGGGGGTCTA CCAGTGCTAC TGCGTGCCTG GATTTCAGGG TTTTCACTGC
    GAGATTGATA TTAATGAGTG TGCATCGCAG CCCTGCGAGA ACAACGGGAC GTGTGTCAAT
    GGCAGAGACA GATACATCTG CGAATGTCTC TTTGGGTTCA CAGGTCTCAA CTGTGAAGTG
    GACGTGGATG AATGTGAAGC GTCTCCCTGC CAGAACGGCG CCGTCTGCCA TGATCACGTG
    GGTCTGTACA CCTGTGAGTG TGTGCCGGGG TACGAGGGCA TCAACTGTGA GCTGGACATC
    GATGAGTGTG AGAGCTCACC CTGCCGCCAT GGAGGAGTCT GTCTGGATAT GGTCAACGGG
    TATGAGTGTG ACTGCAGTGG CACTGGATTC ATGGGCTTTT TCTGTGATAT TGACATCCCC
    GAGTGTTCTT CTGACCCCTG TCAACATGGC GCCACCTGCC TGGAGGGTGT AAACCACTAC
    ACCTGCATCT GCTGGACAGG TTATGAAGGA GTGAACTGTG AGCTGGATGT GGACGAGTGT
    GTTTCTCAGC CATGTGAGAA TGAAGGCCAG TGTTTCCAGC GCTCAGACTC CAGCCTCTAC
    AGAGTTCTGC CTGAACTGGA CACTGACTTC AGCTTCCAGA GCGCAGCCGG TTACCTGTGC
    CAGTGCGTAT CTGGCTTCAC AGGTGAAAAC TGCTCAGTGA ATGTGAATGA ATGCGAATCT
    GCGCCATGTG AGAACGGAGG ATCTTGTGAA GATCTCGTCA ATGCTTTTCA ATGCTCATGT
    CCCCCAGGGT TCGCAGGTGC AGTGTGTGAG ACTGAGCTGG ACGAGTGTGA GAGTGATCCG
    TGTCAGAACG GCGGCCGCTG TGAGGACTCC ATTAATGCCT ACACATGCCA CTGTCCTCCT
    GCTGAGCCGG GACACCTGCC ATGGGGCGGT CCTGACTGCA GCGTCCAGCT GACCGGCTGT
    GTGGACCAAC CCTGCCAAAA CAACGCCACC TGCATCCCTT CAATACAGCA TCAGCAGCAC
    CAGCACACCT GCAGATGCCC ATCCGGCTTC CACGGCACCC ACTGCCAGAC CTCCACCACC
    TTCTCCATCA CCCGCGGAGG ATATTTACTT ATAGATCTTC ATCACAAAAA CAACAGGAGT
    CGCAGATATT TGGATTCACA TGTGCCGAGC GTCCAGCTAC GCTTCCGGAC GACGCTACAG
    GATGGAGTCA TCTTCTTCCG GGGAGGTCAA AATCACTTTT TTGTGCTTGA GTTAATTGCT
    GGAGAGCTGT GCTGCAGAGC TCAGTCTGGA GATTTGAAGC TCTTCGTGAA GCTGAGCGGT
    GATTTCGGGG ATGGCTTCTG GCGAGGGGTT TCTGTGAGTG TGGATGAGGG GCTGACGGTG
    CTTTCAGAGG GTGAGATGGG TGAGGATGGA GGTCACAATC AGCTTCTGTC CTTCCAGCCG
    CAGGGTTTGG AGACACTGTA TGTGGGAGGA GTTCCTCAGG AGTGGATGAA CCTCACACTG
    AGCCGGACGG GGTTTGTGGG CTGTTTGGAG GATCTGGTTG TTGATTCTGA GCCGGTTCTT
    CCTCAGAGTT TGGTCCCGCT GCAGGAGGTT CAGATGGGCT GTGAGAGAAC TGAGTGGTGT
    CGTGTGAACC CCTGCTCTGA TCATGGACGC TGTGTGGATC TGTGGTCAAA CTACACGTGT
    GTCTGCCACA GGCCGTATTA TGGAGAGCAC TGTTCTGAAG AGCACACTTC ATGGACGTTC
    AGTCACGAGC GCAACAGAAG CTTCGCTGCG TTTCCCATCA TGCAGAATCA CAGCCGTGAC
    GTTACGGTGT CTTTATGGCT CAGATCACGC CACTTAAACG GCCTGGTCCT CCAGCTCCAG
    CACGACAATC AACCCTACCT CACTTTATTT CTGAAAAACG GCTCACTGTA TATATCAATA
    TACAGCTCCA TCAGAAGAGC CTCTGACTTC CTGACCAATG GGGAGAAGCT CTTCATGTCC
    ATTAAAACAG AGCCGGGAGT TATATTATTT AACCAAACGC AGCGGATTCC TATGATCTAC
    AGTGGCTTCG AGGTGCAAGT GGGAGATGTT GTGTATCTCG GTGGTCTTCC TGAAGGAGAG
    AACTCTGCGC TGTGGGGAGG ATATTTCAAA GGCTGTTTGC AGGACGTCCG AATCGACGAC
    ACACAACTGT TCATGTACTC CGGCAGCATC AGCCAGAAGC CACAACACGC TAATTACTTA
    CCCAGAAACT CATCCAATGT GCTGGAGGGC TGCGTGGGGG ACCAGACGTG CAAGATGAAG
    CCGTGTCTGA ACGGAGGGAA GTGTCAGGTC ATCTGGAATG ACTTTATGTG CCTCTGTCCG
    CTTAACTTCT CTGGAAAGAC GTGTGAAACT CGTGTCTGGT GTGTTAGCGA CCCCTGTGAC
    CCCAGGACTC GGTGTGTGGA TTTACCTGAC GGTTATGAGT GTTTAGCCAA CGCCACATTT
    GAAAACAACG CCCTGAAGTT CAGCGCAAAC GGCTCATTTT TTGAGCCTGT GATGAGAATT
    TCAATGGATC TGCGGACGCG AGAAGAAAAC GGGACCATTT TACGCTCCTC CAATGGAATC
    GAGTTCTTCT GCATGGGGCT ACTAAACTCC TCCATCATCG TGAAGTTTCG TGATAGTAAT
    AGTCTTGAAG TTCACGCTTT CATCAGCGAC GTCACCGTTT CTGATGGAGA ATGGCATAAA
    GTGGAGCTCT ATGCATCCAG CTCGAGAATA TCAGCGTTTC GTTGGCACAT GTCAATTGAT
    GGAAATCCTG CAGGATTCAG TCTCTCTCCT GCTGGAAATG TAGACTTTTT TAACTCCTCC
    ACTGTGATGC TGGCAGAAAA CTACACTGGG TGTTTGGGCG AAGTGCGCAT CGGTGGAGTT
    CACCTATCAT TTTTCGGACC TCTAAATGAA GAGGCGCCAC AAAAAACTCA ATTCATCCGA
    ATCGGTGGGA TGCTGGAGCC TCATTTAGGA TGCATTGGAT CTCCTTTATG TCTTTCCGAG
    CCCTGCCTGA ATAATGGCAC GTGCACGGAT CTCTTTAATC TTGCCGTGTG TAAATGTGCT
    CCAGGATGGA CCGGCGAGCG CTGCCAGGAG AATATTGACG AATGTGGAGA GCAGCCCTGT
    GTTCAGGGGT CCTGTCAGGA TCTGCTGGGG GATTATGAGT GCCGGTGTCC GGTTGGATTT
    GGTGGGAAGA ACTGTGATGT GGAGGTGGAC CAGTGCAAGG ATCATCAATG CGAGAATGGA
    GGGTCATGTG TGGCTACTGT CAGCGGATAC ACGTGTGTTT GTTTGCCTGG ACACACAGGG
    CCATACTGCC GATGGCGGTT TCCTCCTCGT GAGTGTGATG TGGATCTGCA GTGTGAGAAT
    GGAGGCGTTT GCAGTGAAGG ATCATGGGGA GCCAACTGTA CCTGCAGACC AGGATTCACT
    GGAGTCAGGT GTGAGCTGGA TATAGACGAG TGTGTGTCCG AGCCGTGTCT GAACGGAGGC
    TCATGTGTGA ACAGACACAA TCTCTACTTC TGTGAGTGTT TGCCGGACTT CAGTGGAGAA
    AACTGTCAGA TTACTAAGCA GCTTCAGCAG GAGCTATGGC CGTGGCTGCT GCTCCTGGTC
    CCGCTGGCGG GTCTCTCCGT ACTGCTGGCG GCTCTGGCTC TGCTCTGCCT GATCTTCAGC
    GCCCGGAAGA AGCGCCGCTC GGAGGGAACA TACAGCCCCA GCCGGCAGGA GGCAGCAGGA
    GCACGAATGG AGATGGGCAG CGTGCTGAAG GTGCCTCCGG AAGAGAGACT CATCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.