CACNA2D4A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	CACNA2D4A
Entrez Gene ID	568225
Full Name	calcium channel, voltage-dependent, alpha 2/delta subunit 4a
Synonyms	cacna2d3b,cacna2d4,si:ch211-87p6.2
General protein information	Preferred Names calcium channel, voltage-dependent, alpha 2/delta subunit 4a Names calcium channel, voltage-dependent, alpha 2/delta subunit 4a voltage-dependent calcium channel subunit alpha-2/delta-4 calcium channel, voltage-dependent, alpha 2/delta 3 subunit B calcium channel, voltage-dependent, alpha 2/delta subunit 4
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021475260.1	XP_021330935.1	voltage-dependent calcium channel subunit alpha-2/delta-4 isoform X1
XM_009300430.2	XP_009298705.1	voltage-dependent calcium channel subunit alpha-2/delta-4 isoform X1
XM_017355310.1	XP_017210799.1	voltage-dependent calcium channel subunit alpha-2/delta-4 isoform X3
XM_017355310.2	XP_017210799.1	voltage-dependent calcium channel subunit alpha-2/delta-4 isoform X2
XM_017355309.1	XP_017210798.1	voltage-dependent calcium channel subunit alpha-2/delta-4 isoform X2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cacna2d4	NP_001028554.2
Danio rerio (zebrafish)	cacna2d4a	XP_696635.4
Drosophila melanogaster (fruit fly)	CG4587	NP_001260467.1
Xenopus tropicalis (tropical clawed frog)	cacna2d4	XP_002938150.2
Homo sapiens (human)	CACNA2D4	NP_758952.4
Canis lupus familiaris (dog)	CACNA2D4	XP_534930.2
Bos taurus (cattle)	CACNA2D4	XP_002707867.2
Danio rerio (zebrafish)	cacna2d4b	XP_001920592.4
Pan troglodytes (chimpanzee)	CACNA2D4	XP_001152728.2
Rattus norvegicus (Norway rat)	Cacna2d4	NP_001178680.1
Gallus gallus (chicken)	CACNA2D4	XP_003640465.2
	CACNA2D4	XP_001117901.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009598	XP_318625.3

Gene Ontology

The following CACNA2D4A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CACNA2D4A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa45371	XM_021475260.1 Latest version!	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 4a (cacna2d4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa58571	XM_017355309.1 Latest version!	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 4a (cacna2d4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa58572	XM_017355310.2 Latest version!	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 4a (cacna2d4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa45370	XM_009300430.2 Latest version!	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 4a (cacna2d4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa58572	XM_017355310.1	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 4a (cacna2d4a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CACNA2D4A ( XM_021475260.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021330935.1 Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 4a (cacna2d4a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa45371
Clone ID Related Accession (Same CDS sequence)	XM_021475260.1
Accession Version	XM_021475260.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3318bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent calcium channel subunit alpha-2/delta-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCAGG GGCTGATGGG TAGGATGAGG AGCGTCACAC TGCTCTGGGT GCTGTGTTCA 
AGCTGGACCA TCCAAACTCA AGCTCAGATG AGGATATCAC CTGAAACGGT TCAACAGTGG 
GCCAATAACT TTGGAAAACA GCTTATGGCC TTATCAAACA GATACTCAGG TGCTCAACTC 
TTACAAAAGA AATATAAAGA AGTGGAACCC ATCGTCAAGA TGGAGGAGAT CGATGGTTTA 
AAAATGGTGA AGGAATTTGC TGAGGAAATG GAGAACATGC TGGGCAGAAA AATGAAATCA 
GTTAAGAGAA TGGCAGAGAC AGCAGAAGAC GCAGATTTTT ATCATGAATT TAATGCCACA 
TTAGAGTTTG ACTACTACAA TTCCATGTTG ATTAACACAT TAAATGAAGA TGGAAACAAC 
ATAGAGCTGG GGGGTGAATT TCCTTTGGAG GAGAATGAAC ACTTCAACAA TCTGCCTGTC 
AATACGCAGC AAAGCGTCGT ACAAGTGCCC ACAAATGTTT ATAACAAAGA TCCTGATATC 
CTGAACGGGG TGTTTATGTC AGAAGCACTG AATGACATAT TTATCAGTAA CTTTGAGAAG 
GACCCGACTC TAACCTGGCA GTACTTCGGC AGTTCAACTG GATTCTTTAG ACTTTACCCA 
GGTATTAAGT GGACACCTGA TGAAAATGGA GTTGTCACTT TTGATGGAAG GAATCGCAAC 
TGGTACATTC AAGCAGCCAC ATCTCCTAAA GACATCATTA TCGCTGTGGA CATCAGCGGG 
AGTATGAAGG GTTTGAGACT CACCATCGCA AAACACACCA TCAACACCAT TCTAGACACA 
CTAGGCGAAA ACGACTTTGT CAACGTCATT GCGTACAGTG ATTATGTCCA ATATGTAGAG 
CCCTGTTTTA AAGGCACACT GGTACAAGCT GATCTGGATA ACCGTGAGCA CTTTAAGCTT 
TTGGTGCAGG AGTTGCAGGT AAAAGGTGAA GGAAAGGTGA AGAAAGCCAT GAAAGAGTCC 
TTTAAGATTC TGAATGAGGC AGCAGCAGAA GGCAGGGGTA GTTTATGTAA TCAGGCTATC 
ATGCTGATCA CTGATGGAGC CATGGAGGAT TTCCAGCAGG TGTTTGAGGA GTTCAATTGG 
CCAGATCGCA AGGTGCGCGT GTTCACGTAT CTGATTGGAC GAGAGCTTAC ATTTGCCAGC 
AATGTCAAAT GGATTGCTTG CAATAATAAA GGTTACTACA CTCACATCTC TACACTGGCG 
GATGTACAGG AGAATGTGAT GGAATATCTT CATGTGCTGA GCAGACCAAT GGTCATCAAC 
CATGATCATG ACATCATTTG GACAGAGGCT TACATGGACA GTGTGCTACC CAATACAGCT 
GAGCTTTTTA ACACACAGGC CGAGAGTTTG CTGTTGATGA CCTCTGTAGC CATGCCTGTG 
TTCAGCAAGA AAGAAGAAAC ACTCTCTCAT GGCATCCTCC TCGGTGTGGT TGGCACTGAT 
GTTCCCTTGA AGGAGCTCAT GAGATTGGCT CCTCGATACA AGCTGGGGGT TCACGGCTAT 
GCATTCTTAA ACACTAATAA TGGTTATATA CTCTCTCATC CTGACCTTCG ACCTCTGTAT 
AAAGATGGCA AGAAGCTGAA GCCAAAGCCG AACTATAACA GTGTGGATTT ATCAGAGGTG 
GAGTGGGAGG ATACAGAAGA TGCACTGAGA ACGGCGATGG TAAAAGGGGA AACTGGGACA 
ATCTCTCTGG ACGTCAGAGC AACAGTAGAA AAAGGAAAAA GAGTTGTGTT CCTGAAAAAT 
GAATATTTTT ATACTACCAT CAATGAGACG CCATTTAGTC TTGGGATTGT ACTTACCAAC 
GGTCACGGGA AGAATGTTTT CATCGGAAAT GTTTCAGTTG AAGAAGGCCT ACATGACCTG 
ACTGCGTCTG ATGTCAGTAT TGCTCAAGAA TGGACATACT GTGAGACAGA CATCGATCCA 
CATCATCGCA AATTGACGCA GCTTCAGGCC GTGGTTCGGT ATTTAACAGG AAAAGAACCA 
GATCTGGAAT GTGATGAAAG GCTTCTGCAG CAGACTCTGT TTGACGCAGT GGTCACAGCT 
CCTATGGAAG CCTATTGGAC TGCACTGCTG CTCAACGCCT CCGGTATTGA TGGGGGAGTG 
GAGACAGCGT TCCTGGGCAC GCGCTCTGGC CTCATGAGGA TGATCAGATA TGCTGGCGTA 
GAGAAGCGTG TGGCGAAAAA ATTCTTAACT GCATCAGATA AGGAGAATAT TTTTACTCTA 
GACCATTTCC CTGTTTGGTA TCGGCGTGCG GCAGAAAATC CAGCTGGCCG CTTTTTGTAC 
TACGTTCCTA CAGAGGACAA CTCAGGAGTG AACGGCAGGT TTGTGATTGC TGTCACGCCT 
GTTACAGTCT CTGTGAGCAA AAAGACAGCA GTTGCTGGAG CCATCGGTGT ACAGATGTCT 
CTTGAGATGT TGGAAAAAAG GTTTTGGTCC ATAGCCAACC AGCCGAACGA CACAGATTGT 
TCAGGCATAG AGGGGCTTTG TCCTTTAAGC TGTAGTGATA ATCCGGACAT TAGCTGCTAC 
GTCATTGACA GTAATGGCTT CATCATCATC TCTAGGGACA AGGCTGATGT AGGCAAGTTT 
CTGGGGAAAA TCGAAGGGTC AGTGATGTCC CAACTCTTGA AAATGGGCAT GTTCAAAAGA 
GTTGTACTCT ATGACTATCA GGCCATGTGT AAGGCGCACG CTCACTCTGC TAGTGGCGCT 
CGACCCCTGC TAAGTCCCTT TTATAATCTT TTGGCAGCAC TGAAATGGTT CCTATCCAAC 
TTCCTGTTCC TTTTATTGGA CTTCAACATC TACAATCTGT GGCATTCGGA TCACTTTACA 
GAGGCCAAGA CCTCATTCCA TTCATCTAGT AAGAAGTTAA AGGGTGGCTT TCTGCAGCCG 
TGTGACACTG AATATCCCAG CTTCGTCTAC GAGCCATCAA TCAAAGAAAC CAACAGCCTG 
ATCAAGTGTG GGAGATGTCA AAAGGTGTTC GTAATTCAAC AGATACCAGA TAGCAACTTG 
CTGCTGGTGG TGATTCAGGC TGATTGTGAC TGTTCTCGGC AATATCCACC CATCACCATG 
GAGCCCAAAG AGGTCAAATA CAATGCCTCA GTGAAATGTG ACCGCATGAG ATCTCAGAAG 
ATACGAAGAA GGCCTGAGTC TTGTCACGCT TACCACCCTC AAGAAAATGC CAAGGACTGT 
GGTGGGGCTG TGGCAATCAG CCTGTCTATC ATGCTATTCT TTGCCTGTTT ATCAGTGTCA 
GCACTCATCC ATCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330935.1
CDS	909..4226
Translation	MWQGLMGRMRSVTLLWVLCSSWTIQTQAQMRISPETVQQWANNFGKQLMALSNRYSGAQLLQKKYKEVEPIVKMEEIDGLKMVKEFAEEMENMLGRKMKSVKRMAETAEDADFYHEFNATLEFDYYNSMLINTLNEDGNNIELGGEFPLEENEHFNNLPVNTQQSVVQVPTNVYNKDPDILNGVFMSEALNDIFISNFEKDPTLTWQYFGSSTGFFRLYPGIKWTPDENGVVTFDGRNRNWYIQAATSPKDIIIAVDISGSMKGLRLTIAKHTINTILDTLGENDFVNVIAYSDYVQYVEPCFKGTLVQADLDNREHFKLLVQELQVKGEGKVKKAMKESFKILNEAAAEGRGSLCNQAIMLITDGAMEDFQQVFEEFNWPDRKVRVFTYLIGRELTFASNVKWIACNNKGYYTHISTLADVQENVMEYLHVLSRPMVINHDHDIIWTEAYMDSVLPNTAELFNTQAESLLLMTSVAMPVFSKKEETLSHGILLGVVGTDVPLKELMRLAPRYKLGVHGYAFLNTNNGYILSHPDLRPLYKDGKKLKPKPNYNSVDLSEVEWEDTEDALRTAMVKGETGTISLDVRATVEKGKRVVFLKNEYFYTTINETPFSLGIVLTNGHGKNVFIGNVSVEEGLHDLTASDVSIAQEWTYCETDIDPHHRKLTQLQAVVRYLTGKEPDLECDERLLQQTLFDAVVTAPMEAYWTALLLNASGIDGGVETAFLGTRSGLMRMIRYAGVEKRVAKKFLTASDKENIFTLDHFPVWYRRAAENPAGRFLYYVPTEDNSGVNGRFVIAVTPVTVSVSKKTAVAGAIGVQMSLEMLEKRFWSIANQPNDTDCSGIEGLCPLSCSDNPDISCYVIDSNGFIIISRDKADVGKFLGKIEGSVMSQLLKMGMFKRVVLYDYQAMCKAHAHSASGARPLLSPFYNLLAALKWFLSNFLFLLLDFNIYNLWHSDHFTEAKTSFHSSSKKLKGGFLQPCDTEYPSFVYEPSIKETNSLIKCGRCQKVFVIQQIPDSNLLLVVIQADCDCSRQYPPITMEPKEVKYNASVKCDRMRSQKIRRRPESCHAYHPQENAKDCGGAVAISLSIMLFFACLSVSALIHR

Target ORF information:

RefSeq Version	XM_021475260.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 4a (cacna2d4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475260.1

ORF Insert Sequence:

ATGTGGCAGG GGCTGATGGG TAGGATGAGG AGCGTCACAC TGCTCTGGGT GCTGTGTTCA 
AGCTGGACCA TCCAAACTCA AGCTCAGATG AGGATATCAC CTGAAACGGT TCAACAGTGG 
GCCAATAACT TTGGAAAACA GCTTATGGCC TTATCAAACA GATACTCAGG TGCTCAACTC 
TTACAAAAGA AATATAAAGA AGTGGAACCC ATCGTCAAGA TGGAGGAGAT CGATGGTTTA 
AAAATGGTGA AGGAATTTGC TGAGGAAATG GAGAACATGC TGGGCAGAAA AATGAAATCA 
GTTAAGAGAA TGGCAGAGAC AGCAGAAGAC GCAGATTTTT ATCATGAATT TAATGCCACA 
TTAGAGTTTG ACTACTACAA TTCCATGTTG ATTAACACAT TAAATGAAGA TGGAAACAAC 
ATAGAGCTGG GGGGTGAATT TCCTTTGGAG GAGAATGAAC ACTTCAACAA TCTGCCTGTC 
AATACGCAGC AAAGCGTCGT ACAAGTGCCC ACAAATGTTT ATAACAAAGA TCCTGATATC 
CTGAACGGGG TGTTTATGTC AGAAGCACTG AATGACATAT TTATCAGTAA CTTTGAGAAG 
GACCCGACTC TAACCTGGCA GTACTTCGGC AGTTCAACTG GATTCTTTAG ACTTTACCCA 
GGTATTAAGT GGACACCTGA TGAAAATGGA GTTGTCACTT TTGATGGAAG GAATCGCAAC 
TGGTACATTC AAGCAGCCAC ATCTCCTAAA GACATCATTA TCGCTGTGGA CATCAGCGGG 
AGTATGAAGG GTTTGAGACT CACCATCGCA AAACACACCA TCAACACCAT TCTAGACACA 
CTAGGCGAAA ACGACTTTGT CAACGTCATT GCGTACAGTG ATTATGTCCA ATATGTAGAG 
CCCTGTTTTA AAGGCACACT GGTACAAGCT GATCTGGATA ACCGTGAGCA CTTTAAGCTT 
TTGGTGCAGG AGTTGCAGGT AAAAGGTGAA GGAAAGGTGA AGAAAGCCAT GAAAGAGTCC 
TTTAAGATTC TGAATGAGGC AGCAGCAGAA GGCAGGGGTA GTTTATGTAA TCAGGCTATC 
ATGCTGATCA CTGATGGAGC CATGGAGGAT TTCCAGCAGG TGTTTGAGGA GTTCAATTGG 
CCAGATCGCA AGGTGCGCGT GTTCACGTAT CTGATTGGAC GAGAGCTTAC ATTTGCCAGC 
AATGTCAAAT GGATTGCTTG CAATAATAAA GGTTACTACA CTCACATCTC TACACTGGCG 
GATGTACAGG AGAATGTGAT GGAATATCTT CATGTGCTGA GCAGACCAAT GGTCATCAAC 
CATGATCATG ACATCATTTG GACAGAGGCT TACATGGACA GTGTGCTACC CAATACAGCT 
GAGCTTTTTA ACACACAGGC CGAGAGTTTG CTGTTGATGA CCTCTGTAGC CATGCCTGTG 
TTCAGCAAGA AAGAAGAAAC ACTCTCTCAT GGCATCCTCC TCGGTGTGGT TGGCACTGAT 
GTTCCCTTGA AGGAGCTCAT GAGATTGGCT CCTCGATACA AGCTGGGGGT TCACGGCTAT 
GCATTCTTAA ACACTAATAA TGGTTATATA CTCTCTCATC CTGACCTTCG ACCTCTGTAT 
AAAGATGGCA AGAAGCTGAA GCCAAAGCCG AACTATAACA GTGTGGATTT ATCAGAGGTG 
GAGTGGGAGG ATACAGAAGA TGCACTGAGA ACGGCGATGG TAAAAGGGGA AACTGGGACA 
ATCTCTCTGG ACGTCAGAGC AACAGTAGAA AAAGGAAAAA GAGTTGTGTT CCTGAAAAAT 
GAATATTTTT ATACTACCAT CAATGAGACG CCATTTAGTC TTGGGATTGT ACTTACCAAC 
GGTCACGGGA AGAATGTTTT CATCGGAAAT GTTTCAGTTG AAGAAGGCCT ACATGACCTG 
ACTGCGTCTG ATGTCAGTAT TGCTCAAGAA TGGACATACT GTGAGACAGA CATCGATCCA 
CATCATCGCA AATTGACGCA GCTTCAGGCC GTGGTTCGGT ATTTAACAGG AAAAGAACCA 
GATCTGGAAT GTGATGAAAG GCTTCTGCAG CAGACTCTGT TTGACGCAGT GGTCACAGCT 
CCTATGGAAG CCTATTGGAC TGCACTGCTG CTCAACGCCT CCGGTATTGA TGGGGGAGTG 
GAGACAGCGT TCCTGGGCAC GCGCTCTGGC CTCATGAGGA TGATCAGATA TGCTGGCGTA 
GAGAAGCGTG TGGCGAAAAA ATTCTTAACT GCATCAGATA AGGAGAATAT TTTTACTCTA 
GACCATTTCC CTGTTTGGTA TCGGCGTGCG GCAGAAAATC CAGCTGGCCG CTTTTTGTAC 
TACGTTCCTA CAGAGGACAA CTCAGGAGTG AACGGCAGGT TTGTGATTGC TGTCACGCCT 
GTTACAGTCT CTGTGAGCAA AAAGACAGCA GTTGCTGGAG CCATCGGTGT ACAGATGTCT 
CTTGAGATGT TGGAAAAAAG GTTTTGGTCC ATAGCCAACC AGCCGAACGA CACAGATTGT 
TCAGGCATAG AGGGGCTTTG TCCTTTAAGC TGTAGTGATA ATCCGGACAT TAGCTGCTAC 
GTCATTGACA GTAATGGCTT CATCATCATC TCTAGGGACA AGGCTGATGT AGGCAAGTTT 
CTGGGGAAAA TCGAAGGGTC AGTGATGTCC CAACTCTTGA AAATGGGCAT GTTCAAAAGA 
GTTGTACTCT ATGACTATCA GGCCATGTGT AAGGCGCACG CTCACTCTGC TAGTGGCGCT 
CGACCCCTGC TAAGTCCCTT TTATAATCTT TTGGCAGCAC TGAAATGGTT CCTATCCAAC 
TTCCTGTTCC TTTTATTGGA CTTCAACATC TACAATCTGT GGCATTCGGA TCACTTTACA 
GAGGCCAAGA CCTCATTCCA TTCATCTAGT AAGAAGTTAA AGGGTGGCTT TCTGCAGCCG 
TGTGACACTG AATATCCCAG CTTCGTCTAC GAGCCATCAA TCAAAGAAAC CAACAGCCTG 
ATCAAGTGTG GGAGATGTCA AAAGGTGTTC GTAATTCAAC AGATACCAGA TAGCAACTTG 
CTGCTGGTGG TGATTCAGGC TGATTGTGAC TGTTCTCGGC AATATCCACC CATCACCATG 
GAGCCCAAAG AGGTCAAATA CAATGCCTCA GTGAAATGTG ACCGCATGAG ATCTCAGAAG 
ATACGAAGAA GGCCTGAGTC TTGTCACGCT TACCACCCTC AAGAAAATGC CAAGGACTGT 
GGTGGGGCTG TGGCAATCAG CCTGTCTATC ATGCTATTCT TTGCCTGTTT ATCAGTGTCA 
GCACTCATCC ATCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA2D4A ( XM_017355309.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017210798.1 Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 4a (cacna2d4a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa58571
Clone ID Related Accession (Same CDS sequence)	XM_017355309.1
Accession Version	XM_017355309.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent calcium channel subunit alpha-2/delta-4 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCAGG GGCTGATGGG TAGGATGAGG AGCGTCACAC TGCTCTGGGT GCTGTGTTCA 
AGCTGGACCA TCCAAACTCA AGCTCAGATG AGGATATCAC CTGAAACGGT TCAACAGTGG 
GCCAATAACT TTGGAAAACA GCTTATGGCC TTATCAAACA GATACTCAGG TGCTCAACTC 
TTACAAAAGA AATATAAAGA AGTGGAACCC ATCGTCAAGA TGGAGGAGAT CGATGGTTTA 
AAAATGGTGA AGGAATTTGC TGAGGAAATG GAGAACATGC TGGGCAGAAA AATGAAATCA 
GTTAAGAGAA TGGCAGAGAC AGCAGAAGAC GCAGATTTTT ATCATGAATT TAATGCCACA 
TTAGAGTTTG ACTACTACAA TTCCATGTTG ATTAACACAT TAAATGAAGA TGGAAACAAC 
ATAGAGCTGG GGGGTGAATT TCCTTTGGAG GAGAATGAAC ACTTCAACAA TCTGCCTGTC 
AATACGCAGC AAAGCGTCGT ACAAGTGCCC ACAAATGTTT ATAACAAAGA TCCTGATATC 
CTGAACGGGG TGTTTATGTC AGAAGCACTG AATGACATAT TTATCAGTAA CTTTGAGAAG 
GACCCGACTC TAACCTGGCA GTACTTCGGC AGTTCAACTG GATTCTTTAG ACTTTACCCA 
GGTATTAAGT GGACACCTGA TGAAAATGGA GTTGTCACTT TTGATGGAAG GAATCGCAAC 
TGGTACATTC AAGCAGCCAC ATCTCCTAAA GACATCATTA TCGCTGTGGA CATCAGCGGG 
AGTATGAAGG GTTTGAGACT CACCATCGCA AAACACACCA TCAACACCAT TCTAGACACA 
CTAGGCGAAA ACGACTTTGT CAACGTCATT GCGTACAGTG ATTATGTCCA ATATGTAGAG 
CCCTGTTTTA AAGGCACACT GGTACAAGCT GATCTGGATA ACCGTGAGCA CTTTAAGCTT 
TTGGTGCAGG AGTTGCAGGT AAAAGGTGAA GGAAAGGTGA AGAAAGCCAT GAAAGAGTCC 
TTTAAGATTC TGAATGAGGC AGCAGCAGAA GGCAGGGGTA GTTTATGTAA TCAGGCTATC 
ATGCTGATCA CTGATGGAGC CATGGAGGAT TTCCAGCAGG TGTTTGAGGA GTTCAATTGG 
CCAGATCGCA AGGTGCGCGT GTTCACGTAT CTGATTGGAC GAGAGCTTAC ATTTGCCAGC 
AATGTCAAAT GGATTGCTTG CAATAATAAA GGTTACTACA CTCACATCTC TACACTGGCG 
GATGTACAGG AGAATGTGAT GGAATATCTT CATGTGCTGA GCAGACCAAT GGTCATCAAC 
CATGATCATG ACATCATTTG GACAGAGGCT TACATGGACA GTGTGCTTTT TAACACACAG 
GCCGAGAGTT TGCTGTTGAT GACCTCTGTA GCCATGCCTG TGTTCAGCAA GAAAGAAGAA 
ACACTCTCTC ATGGCATCCT CCTCGGTGTG GTTGGCACTG ATGTTCCCTT GAAGGAGCTC 
ATGAGATTGG CTCCTCGATA CAAGCTGGGG GTTCACGGCT ATGCATTCTT AAACACTAAT 
AATGGTTATA TACTCTCTCA TCCTGACCTT CGACCTCTGT ATAAAGATGG CAAGAAGCTG 
AAGCCAAAGC CGAACTATAA CAGTGTGGAT TTATCAGAGG TGGAGTGGGA GGATACAGAA 
GATGCACTGA GAACGGCGAT GGTAAAAGGG GAAACTGGGA CAATCTCTCT GGACGTCAGA 
GCAACAGTAG AAAAAGGAAA AAGAGTTGTG TTCCTGAAAA ATGAATATTT TTATACTACC 
ATCAATGAGA CGCCATTTAG TCTTGGGATT GTACTTACCA ACGGTCACGG GAAGAATGTT 
TTCATCGGAA ATGTTTCAGT TGAAGAAGGC CTACATGACC TGACTGCGTC TGATGTCAGT 
ATTGCTCAAG AATGGACATA CTGTGAGACA GACATCGATC CACATCATCG CAAATTGACG 
CAGCTTCAGG CCGTGGTTCG GTATTTAACA GGAAAAGAAC CAGATCTGGA ATGTGATGAA 
AGGCTTCTGC AGCAGACTCT GTTTGACGCA GTGGTCACAG CTCCTATGGA AGCCTATTGG 
ACTGCACTGC TGCTCAACGC CTCCGGTATT GATGGGGGAG TGGAGACAGC GTTCCTGGGC 
ACGCGCTCTG GCCTCATGAG GATGATCAGA TATGCTGGCG TAGAGAAGCG TGTGGCGAAA 
AAATTCTTAA CTGCATCAGA TAAGGAGAAT ATTTTTACTC TAGACCATTT CCCTGTTTGG 
TATCGGCGTG CGGCAGAAAA TCCAGCTGGC CGCTTTTTGT ACTACGTTCC TACAGAGGAC 
AACTCAGGAG GTAGCCTGTA CCCTGCAGTC CACATCAAAA CAACTGAGAC TTTACAGCCT 
TTTGAGAGTA CAGTGCTCAG TATTTTTTTC GTTTCTTTCC CTGCAGTGAA CGGCAGGTTT 
GTGATTGCTG TCACGCCTGT TACAGTCTCT GTGAGCAAAA AGACAGCAGT TGCTGGAGCC 
ATCGGTGTAC AGATGTCTCT TGAGATGTTG GAAAAAAGGT TTTGGTCCAT AGCCAACCAG 
CCGAACGACA CAGATTGTTC AGGCATAGAG GGGCTTTGTC CTTTAAGCTG TAGTGATAAT 
CCGGACATTA GCTGCTACGT CATTGACAGT AATGGCTTCA TCATCATCTC TAGGGACAAG 
GCTGATGTAG GCAAGTTTCT GGGGAAAATC GAAGGGTCAG TGATGTCCCA ACTCTTGAAA 
ATGGGCATGT TCAAAAGAGT TGTACTCTAT GACTATCAGG CCATGTGTAA GGCGCACGCT 
CACTCTGCTA GTGGCGCTCG ACCCCTGCTA AGTCCCTTTT ATAATCTTTT GGCAGCACTG 
AAATGGTTCC TATCCAACTT CCTGTTCCTT TTATTGGACT TCAACATCTA CAATCTGTGG 
CATTCGGATC ACTTTACAGA GGCTAGTAAG AAGTTAAAGG GTGGCTTTCT GCAGCCGTGT 
GACACTGAAT ATCCCAGCTT CGTCTACGAG CCATCAATCA AAGAAACCAA CAGCCTGATC 
AAGTGTGGGA GATGTCAAAA GGTGTTCGTA ATTCAACAGA TACCAGATAG CAACTTGCTG 
CTGGTGGTGA TTCAGGCTGA TTGTGACTGT TCTCGGCAAT ATCCACCCAT CACCATGGAG 
CCCAAAGAGG TCAAATACAA TGCCTCAGTG AAATGTGACC GCATGAGATC TCAGAAGATA 
CGAAGAAGGC CTGAGTCTTG TCACGCTTAC CACCCTCAAG AAAATGCCAA GGACTGTGGT 
GGGGCTGTGG CAATCAGCCT GTCTATCATG CTATTCTTTG CCTGTTTATC AGTGTCAGCA 
CTCATCCATC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210798.1
CDS	441..3815
Translation	MWQGLMGRMRSVTLLWVLCSSWTIQTQAQMRISPETVQQWANNFGKQLMALSNRYSGAQLLQKKYKEVEPIVKMEEIDGLKMVKEFAEEMENMLGRKMKSVKRMAETAEDADFYHEFNATLEFDYYNSMLINTLNEDGNNIELGGEFPLEENEHFNNLPVNTQQSVVQVPTNVYNKDPDILNGVFMSEALNDIFISNFEKDPTLTWQYFGSSTGFFRLYPGIKWTPDENGVVTFDGRNRNWYIQAATSPKDIIIAVDISGSMKGLRLTIAKHTINTILDTLGENDFVNVIAYSDYVQYVEPCFKGTLVQADLDNREHFKLLVQELQVKGEGKVKKAMKESFKILNEAAAEGRGSLCNQAIMLITDGAMEDFQQVFEEFNWPDRKVRVFTYLIGRELTFASNVKWIACNNKGYYTHISTLADVQENVMEYLHVLSRPMVINHDHDIIWTEAYMDSVLFNTQAESLLLMTSVAMPVFSKKEETLSHGILLGVVGTDVPLKELMRLAPRYKLGVHGYAFLNTNNGYILSHPDLRPLYKDGKKLKPKPNYNSVDLSEVEWEDTEDALRTAMVKGETGTISLDVRATVEKGKRVVFLKNEYFYTTINETPFSLGIVLTNGHGKNVFIGNVSVEEGLHDLTASDVSIAQEWTYCETDIDPHHRKLTQLQAVVRYLTGKEPDLECDERLLQQTLFDAVVTAPMEAYWTALLLNASGIDGGVETAFLGTRSGLMRMIRYAGVEKRVAKKFLTASDKENIFTLDHFPVWYRRAAENPAGRFLYYVPTEDNSGGSLYPAVHIKTTETLQPFESTVLSIFFVSFPAVNGRFVIAVTPVTVSVSKKTAVAGAIGVQMSLEMLEKRFWSIANQPNDTDCSGIEGLCPLSCSDNPDISCYVIDSNGFIIISRDKADVGKFLGKIEGSVMSQLLKMGMFKRVVLYDYQAMCKAHAHSASGARPLLSPFYNLLAALKWFLSNFLFLLLDFNIYNLWHSDHFTEASKKLKGGFLQPCDTEYPSFVYEPSIKETNSLIKCGRCQKVFVIQQIPDSNLLLVVIQADCDCSRQYPPITMEPKEVKYNASVKCDRMRSQKIRRRPESCHAYHPQENAKDCGGAVAISLSIMLFFACLSVSALIHR

Target ORF information:

RefSeq Version	XM_017355309.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 4a (cacna2d4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355309.1

ORF Insert Sequence:

ATGTGGCAGG GGCTGATGGG TAGGATGAGG AGCGTCACAC TGCTCTGGGT GCTGTGTTCA 
AGCTGGACCA TCCAAACTCA AGCTCAGATG AGGATATCAC CTGAAACGGT TCAACAGTGG 
GCCAATAACT TTGGAAAACA GCTTATGGCC TTATCAAACA GATACTCAGG TGCTCAACTC 
TTACAAAAGA AATATAAAGA AGTGGAACCC ATCGTCAAGA TGGAGGAGAT CGATGGTTTA 
AAAATGGTGA AGGAATTTGC TGAGGAAATG GAGAACATGC TGGGCAGAAA AATGAAATCA 
GTTAAGAGAA TGGCAGAGAC AGCAGAAGAC GCAGATTTTT ATCATGAATT TAATGCCACA 
TTAGAGTTTG ACTACTACAA TTCCATGTTG ATTAACACAT TAAATGAAGA TGGAAACAAC 
ATAGAGCTGG GGGGTGAATT TCCTTTGGAG GAGAATGAAC ACTTCAACAA TCTGCCTGTC 
AATACGCAGC AAAGCGTCGT ACAAGTGCCC ACAAATGTTT ATAACAAAGA TCCTGATATC 
CTGAACGGGG TGTTTATGTC AGAAGCACTG AATGACATAT TTATCAGTAA CTTTGAGAAG 
GACCCGACTC TAACCTGGCA GTACTTCGGC AGTTCAACTG GATTCTTTAG ACTTTACCCA 
GGTATTAAGT GGACACCTGA TGAAAATGGA GTTGTCACTT TTGATGGAAG GAATCGCAAC 
TGGTACATTC AAGCAGCCAC ATCTCCTAAA GACATCATTA TCGCTGTGGA CATCAGCGGG 
AGTATGAAGG GTTTGAGACT CACCATCGCA AAACACACCA TCAACACCAT TCTAGACACA 
CTAGGCGAAA ACGACTTTGT CAACGTCATT GCGTACAGTG ATTATGTCCA ATATGTAGAG 
CCCTGTTTTA AAGGCACACT GGTACAAGCT GATCTGGATA ACCGTGAGCA CTTTAAGCTT 
TTGGTGCAGG AGTTGCAGGT AAAAGGTGAA GGAAAGGTGA AGAAAGCCAT GAAAGAGTCC 
TTTAAGATTC TGAATGAGGC AGCAGCAGAA GGCAGGGGTA GTTTATGTAA TCAGGCTATC 
ATGCTGATCA CTGATGGAGC CATGGAGGAT TTCCAGCAGG TGTTTGAGGA GTTCAATTGG 
CCAGATCGCA AGGTGCGCGT GTTCACGTAT CTGATTGGAC GAGAGCTTAC ATTTGCCAGC 
AATGTCAAAT GGATTGCTTG CAATAATAAA GGTTACTACA CTCACATCTC TACACTGGCG 
GATGTACAGG AGAATGTGAT GGAATATCTT CATGTGCTGA GCAGACCAAT GGTCATCAAC 
CATGATCATG ACATCATTTG GACAGAGGCT TACATGGACA GTGTGCTTTT TAACACACAG 
GCCGAGAGTT TGCTGTTGAT GACCTCTGTA GCCATGCCTG TGTTCAGCAA GAAAGAAGAA 
ACACTCTCTC ATGGCATCCT CCTCGGTGTG GTTGGCACTG ATGTTCCCTT GAAGGAGCTC 
ATGAGATTGG CTCCTCGATA CAAGCTGGGG GTTCACGGCT ATGCATTCTT AAACACTAAT 
AATGGTTATA TACTCTCTCA TCCTGACCTT CGACCTCTGT ATAAAGATGG CAAGAAGCTG 
AAGCCAAAGC CGAACTATAA CAGTGTGGAT TTATCAGAGG TGGAGTGGGA GGATACAGAA 
GATGCACTGA GAACGGCGAT GGTAAAAGGG GAAACTGGGA CAATCTCTCT GGACGTCAGA 
GCAACAGTAG AAAAAGGAAA AAGAGTTGTG TTCCTGAAAA ATGAATATTT TTATACTACC 
ATCAATGAGA CGCCATTTAG TCTTGGGATT GTACTTACCA ACGGTCACGG GAAGAATGTT 
TTCATCGGAA ATGTTTCAGT TGAAGAAGGC CTACATGACC TGACTGCGTC TGATGTCAGT 
ATTGCTCAAG AATGGACATA CTGTGAGACA GACATCGATC CACATCATCG CAAATTGACG 
CAGCTTCAGG CCGTGGTTCG GTATTTAACA GGAAAAGAAC CAGATCTGGA ATGTGATGAA 
AGGCTTCTGC AGCAGACTCT GTTTGACGCA GTGGTCACAG CTCCTATGGA AGCCTATTGG 
ACTGCACTGC TGCTCAACGC CTCCGGTATT GATGGGGGAG TGGAGACAGC GTTCCTGGGC 
ACGCGCTCTG GCCTCATGAG GATGATCAGA TATGCTGGCG TAGAGAAGCG TGTGGCGAAA 
AAATTCTTAA CTGCATCAGA TAAGGAGAAT ATTTTTACTC TAGACCATTT CCCTGTTTGG 
TATCGGCGTG CGGCAGAAAA TCCAGCTGGC CGCTTTTTGT ACTACGTTCC TACAGAGGAC 
AACTCAGGAG GTAGCCTGTA CCCTGCAGTC CACATCAAAA CAACTGAGAC TTTACAGCCT 
TTTGAGAGTA CAGTGCTCAG TATTTTTTTC GTTTCTTTCC CTGCAGTGAA CGGCAGGTTT 
GTGATTGCTG TCACGCCTGT TACAGTCTCT GTGAGCAAAA AGACAGCAGT TGCTGGAGCC 
ATCGGTGTAC AGATGTCTCT TGAGATGTTG GAAAAAAGGT TTTGGTCCAT AGCCAACCAG 
CCGAACGACA CAGATTGTTC AGGCATAGAG GGGCTTTGTC CTTTAAGCTG TAGTGATAAT 
CCGGACATTA GCTGCTACGT CATTGACAGT AATGGCTTCA TCATCATCTC TAGGGACAAG 
GCTGATGTAG GCAAGTTTCT GGGGAAAATC GAAGGGTCAG TGATGTCCCA ACTCTTGAAA 
ATGGGCATGT TCAAAAGAGT TGTACTCTAT GACTATCAGG CCATGTGTAA GGCGCACGCT 
CACTCTGCTA GTGGCGCTCG ACCCCTGCTA AGTCCCTTTT ATAATCTTTT GGCAGCACTG 
AAATGGTTCC TATCCAACTT CCTGTTCCTT TTATTGGACT TCAACATCTA CAATCTGTGG 
CATTCGGATC ACTTTACAGA GGCTAGTAAG AAGTTAAAGG GTGGCTTTCT GCAGCCGTGT 
GACACTGAAT ATCCCAGCTT CGTCTACGAG CCATCAATCA AAGAAACCAA CAGCCTGATC 
AAGTGTGGGA GATGTCAAAA GGTGTTCGTA ATTCAACAGA TACCAGATAG CAACTTGCTG 
CTGGTGGTGA TTCAGGCTGA TTGTGACTGT TCTCGGCAAT ATCCACCCAT CACCATGGAG 
CCCAAAGAGG TCAAATACAA TGCCTCAGTG AAATGTGACC GCATGAGATC TCAGAAGATA 
CGAAGAAGGC CTGAGTCTTG TCACGCTTAC CACCCTCAAG AAAATGCCAA GGACTGTGGT 
GGGGCTGTGG CAATCAGCCT GTCTATCATG CTATTCTTTG CCTGTTTATC AGTGTCAGCA 
CTCATCCATC GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA2D4A ( XM_017355310.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017210799.1 Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 4a (cacna2d4a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa58572
Clone ID Related Accession (Same CDS sequence)	XM_017355310.1 , XM_017355310.2
Accession Version	XM_017355310.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent calcium channel subunit alpha-2/delta-4 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCAGG GGCTGATGGG TAGGATGAGG AGCGTCACAC TGCTCTGGGT GCTGTGTTCA 
AGCTGGACCA TCCAAACTCA AGCTCAGATG AGGATATCAC CTGAAACGGT TCAACAGTGG 
GCCAATAACT TTGGAAAACA GCTTATGGCC TTATCAAACA GATACTCAGG TGCTCAACTC 
TTACAAAAGA AATATAAAGA AGTGGAACCC ATCGTCAAGA TGGAGGAGAT CGATGGTTTA 
AAAATGGTGA AGGAATTTGC TGAGGAAATG GAGAACATGC TGGGCAGAAA AATGAAATCA 
GTTAAGAGAA TGGCAGAGAC AGCAGAAGAC GCAGATTTTT ATCATGAATT TAATGCCACA 
TTAGAGTTTG ACTACTACAA TTCCATGTTG ATTAACACAT TAAATGAAGA TGGAAACAAC 
ATAGAGCTGG GGGGTGAATT TCCTTTGGAG GAGAATGAAC ACTTCAACAA TCTGCCTGTC 
AATACGCAGC AAAGCGTCGT ACAAGTGCCC ACAAATGTTT ATAACAAAGA TCCTGATATC 
CTGAACGGGG TGTTTATGTC AGAAGCACTG AATGACATAT TTATCAGTAA CTTTGAGAAG 
GACCCGACTC TAACCTGGCA GTACTTCGGC AGTTCAACTG GATTCTTTAG ACTTTACCCA 
GGTATTAAGT GGACACCTGA TGAAAATGGA GTTGTCACTT TTGATGGAAG GAATCGCAAC 
TGGTACATTC AAGCAGCCAC ATCTCCTAAA GACATCATTA TCGCTGTGGA CATCAGCGGG 
AGTATGAAGG GTTTGAGACT CACCATCGCA AAACACACCA TCAACACCAT TCTAGACACA 
CTAGGCGAAA ACGACTTTGT CAACGTCATT GCGTACAGTG ATTATGTCCA ATATGTAGAG 
CCCTGTTTTA AAGGCACACT GGTACAAGCT GATCTGGATA ACCGTGAGCA CTTTAAGCTT 
TTGGTGCAGG AGTTGCAGGT AAAAGGTGAA GGAAAGGTGA AGAAAGCCAT GAAAGAGTCC 
TTTAAGATTC TGAATGAGGC AGCAGCAGAA GGCAGGGGTA GTTTATGTAA TCAGGCTATC 
ATGCTGATCA CTGATGGAGC CATGGAGGAT TTCCAGCAGG TGTTTGAGGA GTTCAATTGG 
CCAGATCGCA AGGTGCGCGT GTTCACGTAT CTGATTGGAC GAGAGCTTAC ATTTGCCAGC 
AATGTCAAAT GGATTGCTTG CAATAATAAA GGTTACTACA CTCACATCTC TACACTGGCG 
GATGTACAGG AGAATGTGAT GGAATATCTT CATGTGCTGA GCAGACCAAT GGTCATCAAC 
CATGATCATG ACATCATTTG GACAGAGGCT TACATGGACA GTGTGCTTTT TAACACACAG 
GCCGAGAGTT TGCTGTTGAT GACCTCTGTA GCCATGCCTG TGTTCAGCAA GAAAGAAGAA 
ACACTCTCTC ATGGCATCCT CCTCGGTGTG GTTGGCACTG ATGTTCCCTT GAAGGAGCTC 
ATGAGATTGG CTCCTCGATA CAAGCTGGGG GTTCACGGCT ATGCATTCTT AAACACTAAT 
AATGGTTATA TACTCTCTCA TCCTGACCTT CGACCTCTGT ATAAAGATGG CAAGAAGCTG 
AAGCCAAAGC CGAACTATAA CAGTGTGGAT TTATCAGAGG TGGAGTGGGA GGATACAGAA 
GATGCACTGA GAACGGCGAT GGTAAAAGGG GAAACTGGGA CAATCTCTCT GGACGTCAGA 
GCAACAGTAG AAAAAGGAAA AAGAGTTGTG TTCCTGAAAA ATGAATATTT TTATACTACC 
ATCAATGAGA CGCCATTTAG TCTTGGGATT GTACTTACCA ACGGTCACGG GAAGAATGTT 
TTCATCGGAA ATGTTTCAGT TGAAGAAGGC CTACATGACC TGACTGCGTC TGATGTCAGT 
ATTGCTCAAG AATGGACATA CTGTGAGACA GACATCGATC CACATCATCG CAAATTGACG 
CAGCTTCAGG CCGTGGTTCG GTATTTAACA GGAAAAGAAC CAGATCTGGA ATGTGATGAA 
AGGCTTCTGC AGCAGACTCT GTTTGACGCA GTGGTCACAG CTCCTATGGA AGCCTATTGG 
ACTGCACTGC TGCTCAACGC CTCCGGTATT GATGGGGGAG TGGAGACAGC GTTCCTGGGC 
ACGCGCTCTG GCCTCATGAG GATGATCAGA TATGCTGGCG TAGAGAAGCG TGTGGCGAAA 
AAATTCTTAA CTGCATCAGA TAAGGAGAAT ATTTTTACTC TAGACCATTT CCCTGTTTGG 
TATCGGCGTG CGGCAGAAAA TCCAGCTGGC CGCTTTTTGT ACTACGTTCC TACAGAGGAC 
AACTCAGGAG TGAACGGCAG GTTTGTGATT GCTGTCACGC CTGTTACAGT CTCTGTGAGC 
AAAAAGACAG CAGTTGCTGG AGCCATCGGT GTACAGATGT CTCTTGAGAT GTTGGAAAAA 
AGGTTTTGGT CCATAGCCAA CCAGCCGAAC GACACAGATT GTTCAGGCAT AGAGGGGCTT 
TGTCCTTTAA GCTGTAGTGA TAATCCGGAC ATTAGCTGCT ACGTCATTGA CAGTAATGGC 
TTCATCATCA TCTCTAGGGA CAAGGCTGAT GTAGGCAAGT TTCTGGGGAA AATCGAAGGG 
TCAGTGATGT CCCAACTCTT GAAAATGGGC ATGTTCAAAA GAGTTGTACT CTATGACTAT 
CAGGCCATGT GTAAGGCGCA CGCTCACTCT GCTAGTGGCG CTCGACCCCT GCTAAGTCCC 
TTTTATAATC TTTTGGCAGC ACTGAAATGG TTCCTATCCA ACTTCCTGTT CCTTTTATTG 
GACTTCAACA TCTACAATCT GTGGCATTCG GATCACTTTA CAGAGGCCAA GACCTCATTC 
CATTCATCTA GTAAGAAGTT AAAGGGTGGC TTTCTGCAGC CGTGTGACAC TGAATATCCC 
AGCTTCGTCT ACGAGCCATC AATCAAAGAA ACCAACAGCC TGATCAAGTG TGGGAGATGT 
CAAAAGGTGT TCGTAATTCA ACAGATACCA GATAGCAACT TGCTGCTGGT GGTGATTCAG 
GCTGATTGTG ACTGTTCTCG GCAATATCCA CCCATCACCA TGGAGCCCAA AGAGGTCAAA 
TACAATGCCT CAGTGAAATG TGACCGCATG AGATCTCAGA AGATACGAAG AAGGCCTGAG 
TCTTGTCACG CTTACCACCC TCAAGAAAAT GCCAAGGACT GTGGTGGGGC TGTGGCAATC 
AGCCTGTCTA TCATGCTATT CTTTGCCTGT TTATCAGTGT CAGCACTCAT CCATCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210799.1
CDS	441..3740
Translation	MWQGLMGRMRSVTLLWVLCSSWTIQTQAQMRISPETVQQWANNFGKQLMALSNRYSGAQLLQKKYKEVEPIVKMEEIDGLKMVKEFAEEMENMLGRKMKSVKRMAETAEDADFYHEFNATLEFDYYNSMLINTLNEDGNNIELGGEFPLEENEHFNNLPVNTQQSVVQVPTNVYNKDPDILNGVFMSEALNDIFISNFEKDPTLTWQYFGSSTGFFRLYPGIKWTPDENGVVTFDGRNRNWYIQAATSPKDIIIAVDISGSMKGLRLTIAKHTINTILDTLGENDFVNVIAYSDYVQYVEPCFKGTLVQADLDNREHFKLLVQELQVKGEGKVKKAMKESFKILNEAAAEGRGSLCNQAIMLITDGAMEDFQQVFEEFNWPDRKVRVFTYLIGRELTFASNVKWIACNNKGYYTHISTLADVQENVMEYLHVLSRPMVINHDHDIIWTEAYMDSVLFNTQAESLLLMTSVAMPVFSKKEETLSHGILLGVVGTDVPLKELMRLAPRYKLGVHGYAFLNTNNGYILSHPDLRPLYKDGKKLKPKPNYNSVDLSEVEWEDTEDALRTAMVKGETGTISLDVRATVEKGKRVVFLKNEYFYTTINETPFSLGIVLTNGHGKNVFIGNVSVEEGLHDLTASDVSIAQEWTYCETDIDPHHRKLTQLQAVVRYLTGKEPDLECDERLLQQTLFDAVVTAPMEAYWTALLLNASGIDGGVETAFLGTRSGLMRMIRYAGVEKRVAKKFLTASDKENIFTLDHFPVWYRRAAENPAGRFLYYVPTEDNSGVNGRFVIAVTPVTVSVSKKTAVAGAIGVQMSLEMLEKRFWSIANQPNDTDCSGIEGLCPLSCSDNPDISCYVIDSNGFIIISRDKADVGKFLGKIEGSVMSQLLKMGMFKRVVLYDYQAMCKAHAHSASGARPLLSPFYNLLAALKWFLSNFLFLLLDFNIYNLWHSDHFTEAKTSFHSSSKKLKGGFLQPCDTEYPSFVYEPSIKETNSLIKCGRCQKVFVIQQIPDSNLLLVVIQADCDCSRQYPPITMEPKEVKYNASVKCDRMRSQKIRRRPESCHAYHPQENAKDCGGAVAISLSIMLFFACLSVSALIHR

Target ORF information:

RefSeq Version	XM_017355310.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 4a (cacna2d4a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355310.1

ORF Insert Sequence:

ATGTGGCAGG GGCTGATGGG TAGGATGAGG AGCGTCACAC TGCTCTGGGT GCTGTGTTCA 
AGCTGGACCA TCCAAACTCA AGCTCAGATG AGGATATCAC CTGAAACGGT TCAACAGTGG 
GCCAATAACT TTGGAAAACA GCTTATGGCC TTATCAAACA GATACTCAGG TGCTCAACTC 
TTACAAAAGA AATATAAAGA AGTGGAACCC ATCGTCAAGA TGGAGGAGAT CGATGGTTTA 
AAAATGGTGA AGGAATTTGC TGAGGAAATG GAGAACATGC TGGGCAGAAA AATGAAATCA 
GTTAAGAGAA TGGCAGAGAC AGCAGAAGAC GCAGATTTTT ATCATGAATT TAATGCCACA 
TTAGAGTTTG ACTACTACAA TTCCATGTTG ATTAACACAT TAAATGAAGA TGGAAACAAC 
ATAGAGCTGG GGGGTGAATT TCCTTTGGAG GAGAATGAAC ACTTCAACAA TCTGCCTGTC 
AATACGCAGC AAAGCGTCGT ACAAGTGCCC ACAAATGTTT ATAACAAAGA TCCTGATATC 
CTGAACGGGG TGTTTATGTC AGAAGCACTG AATGACATAT TTATCAGTAA CTTTGAGAAG 
GACCCGACTC TAACCTGGCA GTACTTCGGC AGTTCAACTG GATTCTTTAG ACTTTACCCA 
GGTATTAAGT GGACACCTGA TGAAAATGGA GTTGTCACTT TTGATGGAAG GAATCGCAAC 
TGGTACATTC AAGCAGCCAC ATCTCCTAAA GACATCATTA TCGCTGTGGA CATCAGCGGG 
AGTATGAAGG GTTTGAGACT CACCATCGCA AAACACACCA TCAACACCAT TCTAGACACA 
CTAGGCGAAA ACGACTTTGT CAACGTCATT GCGTACAGTG ATTATGTCCA ATATGTAGAG 
CCCTGTTTTA AAGGCACACT GGTACAAGCT GATCTGGATA ACCGTGAGCA CTTTAAGCTT 
TTGGTGCAGG AGTTGCAGGT AAAAGGTGAA GGAAAGGTGA AGAAAGCCAT GAAAGAGTCC 
TTTAAGATTC TGAATGAGGC AGCAGCAGAA GGCAGGGGTA GTTTATGTAA TCAGGCTATC 
ATGCTGATCA CTGATGGAGC CATGGAGGAT TTCCAGCAGG TGTTTGAGGA GTTCAATTGG 
CCAGATCGCA AGGTGCGCGT GTTCACGTAT CTGATTGGAC GAGAGCTTAC ATTTGCCAGC 
AATGTCAAAT GGATTGCTTG CAATAATAAA GGTTACTACA CTCACATCTC TACACTGGCG 
GATGTACAGG AGAATGTGAT GGAATATCTT CATGTGCTGA GCAGACCAAT GGTCATCAAC 
CATGATCATG ACATCATTTG GACAGAGGCT TACATGGACA GTGTGCTTTT TAACACACAG 
GCCGAGAGTT TGCTGTTGAT GACCTCTGTA GCCATGCCTG TGTTCAGCAA GAAAGAAGAA 
ACACTCTCTC ATGGCATCCT CCTCGGTGTG GTTGGCACTG ATGTTCCCTT GAAGGAGCTC 
ATGAGATTGG CTCCTCGATA CAAGCTGGGG GTTCACGGCT ATGCATTCTT AAACACTAAT 
AATGGTTATA TACTCTCTCA TCCTGACCTT CGACCTCTGT ATAAAGATGG CAAGAAGCTG 
AAGCCAAAGC CGAACTATAA CAGTGTGGAT TTATCAGAGG TGGAGTGGGA GGATACAGAA 
GATGCACTGA GAACGGCGAT GGTAAAAGGG GAAACTGGGA CAATCTCTCT GGACGTCAGA 
GCAACAGTAG AAAAAGGAAA AAGAGTTGTG TTCCTGAAAA ATGAATATTT TTATACTACC 
ATCAATGAGA CGCCATTTAG TCTTGGGATT GTACTTACCA ACGGTCACGG GAAGAATGTT 
TTCATCGGAA ATGTTTCAGT TGAAGAAGGC CTACATGACC TGACTGCGTC TGATGTCAGT 
ATTGCTCAAG AATGGACATA CTGTGAGACA GACATCGATC CACATCATCG CAAATTGACG 
CAGCTTCAGG CCGTGGTTCG GTATTTAACA GGAAAAGAAC CAGATCTGGA ATGTGATGAA 
AGGCTTCTGC AGCAGACTCT GTTTGACGCA GTGGTCACAG CTCCTATGGA AGCCTATTGG 
ACTGCACTGC TGCTCAACGC CTCCGGTATT GATGGGGGAG TGGAGACAGC GTTCCTGGGC 
ACGCGCTCTG GCCTCATGAG GATGATCAGA TATGCTGGCG TAGAGAAGCG TGTGGCGAAA 
AAATTCTTAA CTGCATCAGA TAAGGAGAAT ATTTTTACTC TAGACCATTT CCCTGTTTGG 
TATCGGCGTG CGGCAGAAAA TCCAGCTGGC CGCTTTTTGT ACTACGTTCC TACAGAGGAC 
AACTCAGGAG TGAACGGCAG GTTTGTGATT GCTGTCACGC CTGTTACAGT CTCTGTGAGC 
AAAAAGACAG CAGTTGCTGG AGCCATCGGT GTACAGATGT CTCTTGAGAT GTTGGAAAAA 
AGGTTTTGGT CCATAGCCAA CCAGCCGAAC GACACAGATT GTTCAGGCAT AGAGGGGCTT 
TGTCCTTTAA GCTGTAGTGA TAATCCGGAC ATTAGCTGCT ACGTCATTGA CAGTAATGGC 
TTCATCATCA TCTCTAGGGA CAAGGCTGAT GTAGGCAAGT TTCTGGGGAA AATCGAAGGG 
TCAGTGATGT CCCAACTCTT GAAAATGGGC ATGTTCAAAA GAGTTGTACT CTATGACTAT 
CAGGCCATGT GTAAGGCGCA CGCTCACTCT GCTAGTGGCG CTCGACCCCT GCTAAGTCCC 
TTTTATAATC TTTTGGCAGC ACTGAAATGG TTCCTATCCA ACTTCCTGTT CCTTTTATTG 
GACTTCAACA TCTACAATCT GTGGCATTCG GATCACTTTA CAGAGGCCAA GACCTCATTC 
CATTCATCTA GTAAGAAGTT AAAGGGTGGC TTTCTGCAGC CGTGTGACAC TGAATATCCC 
AGCTTCGTCT ACGAGCCATC AATCAAAGAA ACCAACAGCC TGATCAAGTG TGGGAGATGT 
CAAAAGGTGT TCGTAATTCA ACAGATACCA GATAGCAACT TGCTGCTGGT GGTGATTCAG 
GCTGATTGTG ACTGTTCTCG GCAATATCCA CCCATCACCA TGGAGCCCAA AGAGGTCAAA 
TACAATGCCT CAGTGAAATG TGACCGCATG AGATCTCAGA AGATACGAAG AAGGCCTGAG 
TCTTGTCACG CTTACCACCC TCAAGAAAAT GCCAAGGACT GTGGTGGGGC TGTGGCAATC 
AGCCTGTCTA TCATGCTATT CTTTGCCTGT TTATCAGTGT CAGCACTCAT CCATCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA2D4A ( XM_017355310.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017210799.1 Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 4a (cacna2d4a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa58572
Clone ID Related Accession (Same CDS sequence)	XM_017355310.1 , XM_017355310.2
Accession Version	XM_017355310.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent calcium channel subunit alpha-2/delta-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017355310.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCAGG GGCTGATGGG TAGGATGAGG AGCGTCACAC TGCTCTGGGT GCTGTGTTCA 
AGCTGGACCA TCCAAACTCA AGCTCAGATG AGGATATCAC CTGAAACGGT TCAACAGTGG 
GCCAATAACT TTGGAAAACA GCTTATGGCC TTATCAAACA GATACTCAGG TGCTCAACTC 
TTACAAAAGA AATATAAAGA AGTGGAACCC ATCGTCAAGA TGGAGGAGAT CGATGGTTTA 
AAAATGGTGA AGGAATTTGC TGAGGAAATG GAGAACATGC TGGGCAGAAA AATGAAATCA 
GTTAAGAGAA TGGCAGAGAC AGCAGAAGAC GCAGATTTTT ATCATGAATT TAATGCCACA 
TTAGAGTTTG ACTACTACAA TTCCATGTTG ATTAACACAT TAAATGAAGA TGGAAACAAC 
ATAGAGCTGG GGGGTGAATT TCCTTTGGAG GAGAATGAAC ACTTCAACAA TCTGCCTGTC 
AATACGCAGC AAAGCGTCGT ACAAGTGCCC ACAAATGTTT ATAACAAAGA TCCTGATATC 
CTGAACGGGG TGTTTATGTC AGAAGCACTG AATGACATAT TTATCAGTAA CTTTGAGAAG 
GACCCGACTC TAACCTGGCA GTACTTCGGC AGTTCAACTG GATTCTTTAG ACTTTACCCA 
GGTATTAAGT GGACACCTGA TGAAAATGGA GTTGTCACTT TTGATGGAAG GAATCGCAAC 
TGGTACATTC AAGCAGCCAC ATCTCCTAAA GACATCATTA TCGCTGTGGA CATCAGCGGG 
AGTATGAAGG GTTTGAGACT CACCATCGCA AAACACACCA TCAACACCAT TCTAGACACA 
CTAGGCGAAA ACGACTTTGT CAACGTCATT GCGTACAGTG ATTATGTCCA ATATGTAGAG 
CCCTGTTTTA AAGGCACACT GGTACAAGCT GATCTGGATA ACCGTGAGCA CTTTAAGCTT 
TTGGTGCAGG AGTTGCAGGT AAAAGGTGAA GGAAAGGTGA AGAAAGCCAT GAAAGAGTCC 
TTTAAGATTC TGAATGAGGC AGCAGCAGAA GGCAGGGGTA GTTTATGTAA TCAGGCTATC 
ATGCTGATCA CTGATGGAGC CATGGAGGAT TTCCAGCAGG TGTTTGAGGA GTTCAATTGG 
CCAGATCGCA AGGTGCGCGT GTTCACGTAT CTGATTGGAC GAGAGCTTAC ATTTGCCAGC 
AATGTCAAAT GGATTGCTTG CAATAATAAA GGTTACTACA CTCACATCTC TACACTGGCG 
GATGTACAGG AGAATGTGAT GGAATATCTT CATGTGCTGA GCAGACCAAT GGTCATCAAC 
CATGATCATG ACATCATTTG GACAGAGGCT TACATGGACA GTGTGCTTTT TAACACACAG 
GCCGAGAGTT TGCTGTTGAT GACCTCTGTA GCCATGCCTG TGTTCAGCAA GAAAGAAGAA 
ACACTCTCTC ATGGCATCCT CCTCGGTGTG GTTGGCACTG ATGTTCCCTT GAAGGAGCTC 
ATGAGATTGG CTCCTCGATA CAAGCTGGGG GTTCACGGCT ATGCATTCTT AAACACTAAT 
AATGGTTATA TACTCTCTCA TCCTGACCTT CGACCTCTGT ATAAAGATGG CAAGAAGCTG 
AAGCCAAAGC CGAACTATAA CAGTGTGGAT TTATCAGAGG TGGAGTGGGA GGATACAGAA 
GATGCACTGA GAACGGCGAT GGTAAAAGGG GAAACTGGGA CAATCTCTCT GGACGTCAGA 
GCAACAGTAG AAAAAGGAAA AAGAGTTGTG TTCCTGAAAA ATGAATATTT TTATACTACC 
ATCAATGAGA CGCCATTTAG TCTTGGGATT GTACTTACCA ACGGTCACGG GAAGAATGTT 
TTCATCGGAA ATGTTTCAGT TGAAGAAGGC CTACATGACC TGACTGCGTC TGATGTCAGT 
ATTGCTCAAG AATGGACATA CTGTGAGACA GACATCGATC CACATCATCG CAAATTGACG 
CAGCTTCAGG CCGTGGTTCG GTATTTAACA GGAAAAGAAC CAGATCTGGA ATGTGATGAA 
AGGCTTCTGC AGCAGACTCT GTTTGACGCA GTGGTCACAG CTCCTATGGA AGCCTATTGG 
ACTGCACTGC TGCTCAACGC CTCCGGTATT GATGGGGGAG TGGAGACAGC GTTCCTGGGC 
ACGCGCTCTG GCCTCATGAG GATGATCAGA TATGCTGGCG TAGAGAAGCG TGTGGCGAAA 
AAATTCTTAA CTGCATCAGA TAAGGAGAAT ATTTTTACTC TAGACCATTT CCCTGTTTGG 
TATCGGCGTG CGGCAGAAAA TCCAGCTGGC CGCTTTTTGT ACTACGTTCC TACAGAGGAC 
AACTCAGGAG TGAACGGCAG GTTTGTGATT GCTGTCACGC CTGTTACAGT CTCTGTGAGC 
AAAAAGACAG CAGTTGCTGG AGCCATCGGT GTACAGATGT CTCTTGAGAT GTTGGAAAAA 
AGGTTTTGGT CCATAGCCAA CCAGCCGAAC GACACAGATT GTTCAGGCAT AGAGGGGCTT 
TGTCCTTTAA GCTGTAGTGA TAATCCGGAC ATTAGCTGCT ACGTCATTGA CAGTAATGGC 
TTCATCATCA TCTCTAGGGA CAAGGCTGAT GTAGGCAAGT TTCTGGGGAA AATCGAAGGG 
TCAGTGATGT CCCAACTCTT GAAAATGGGC ATGTTCAAAA GAGTTGTACT CTATGACTAT 
CAGGCCATGT GTAAGGCGCA CGCTCACTCT GCTAGTGGCG CTCGACCCCT GCTAAGTCCC 
TTTTATAATC TTTTGGCAGC ACTGAAATGG TTCCTATCCA ACTTCCTGTT CCTTTTATTG 
GACTTCAACA TCTACAATCT GTGGCATTCG GATCACTTTA CAGAGGCCAA GACCTCATTC 
CATTCATCTA GTAAGAAGTT AAAGGGTGGC TTTCTGCAGC CGTGTGACAC TGAATATCCC 
AGCTTCGTCT ACGAGCCATC AATCAAAGAA ACCAACAGCC TGATCAAGTG TGGGAGATGT 
CAAAAGGTGT TCGTAATTCA ACAGATACCA GATAGCAACT TGCTGCTGGT GGTGATTCAG 
GCTGATTGTG ACTGTTCTCG GCAATATCCA CCCATCACCA TGGAGCCCAA AGAGGTCAAA 
TACAATGCCT CAGTGAAATG TGACCGCATG AGATCTCAGA AGATACGAAG AAGGCCTGAG 
TCTTGTCACG CTTACCACCC TCAAGAAAAT GCCAAGGACT GTGGTGGGGC TGTGGCAATC 
AGCCTGTCTA TCATGCTATT CTTTGCCTGT TTATCAGTGT CAGCACTCAT CCATCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210799.1
CDS	909..4208
Translation	MWQGLMGRMRSVTLLWVLCSSWTIQTQAQMRISPETVQQWANNFGKQLMALSNRYSGAQLLQKKYKEVEPIVKMEEIDGLKMVKEFAEEMENMLGRKMKSVKRMAETAEDADFYHEFNATLEFDYYNSMLINTLNEDGNNIELGGEFPLEENEHFNNLPVNTQQSVVQVPTNVYNKDPDILNGVFMSEALNDIFISNFEKDPTLTWQYFGSSTGFFRLYPGIKWTPDENGVVTFDGRNRNWYIQAATSPKDIIIAVDISGSMKGLRLTIAKHTINTILDTLGENDFVNVIAYSDYVQYVEPCFKGTLVQADLDNREHFKLLVQELQVKGEGKVKKAMKESFKILNEAAAEGRGSLCNQAIMLITDGAMEDFQQVFEEFNWPDRKVRVFTYLIGRELTFASNVKWIACNNKGYYTHISTLADVQENVMEYLHVLSRPMVINHDHDIIWTEAYMDSVLFNTQAESLLLMTSVAMPVFSKKEETLSHGILLGVVGTDVPLKELMRLAPRYKLGVHGYAFLNTNNGYILSHPDLRPLYKDGKKLKPKPNYNSVDLSEVEWEDTEDALRTAMVKGETGTISLDVRATVEKGKRVVFLKNEYFYTTINETPFSLGIVLTNGHGKNVFIGNVSVEEGLHDLTASDVSIAQEWTYCETDIDPHHRKLTQLQAVVRYLTGKEPDLECDERLLQQTLFDAVVTAPMEAYWTALLLNASGIDGGVETAFLGTRSGLMRMIRYAGVEKRVAKKFLTASDKENIFTLDHFPVWYRRAAENPAGRFLYYVPTEDNSGVNGRFVIAVTPVTVSVSKKTAVAGAIGVQMSLEMLEKRFWSIANQPNDTDCSGIEGLCPLSCSDNPDISCYVIDSNGFIIISRDKADVGKFLGKIEGSVMSQLLKMGMFKRVVLYDYQAMCKAHAHSASGARPLLSPFYNLLAALKWFLSNFLFLLLDFNIYNLWHSDHFTEAKTSFHSSSKKLKGGFLQPCDTEYPSFVYEPSIKETNSLIKCGRCQKVFVIQQIPDSNLLLVVIQADCDCSRQYPPITMEPKEVKYNASVKCDRMRSQKIRRRPESCHAYHPQENAKDCGGAVAISLSIMLFFACLSVSALIHR

Target ORF information:

RefSeq Version	XM_017355310.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 4a (cacna2d4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355310.2

ORF Insert Sequence:

ATGTGGCAGG GGCTGATGGG TAGGATGAGG AGCGTCACAC TGCTCTGGGT GCTGTGTTCA 
AGCTGGACCA TCCAAACTCA AGCTCAGATG AGGATATCAC CTGAAACGGT TCAACAGTGG 
GCCAATAACT TTGGAAAACA GCTTATGGCC TTATCAAACA GATACTCAGG TGCTCAACTC 
TTACAAAAGA AATATAAAGA AGTGGAACCC ATCGTCAAGA TGGAGGAGAT CGATGGTTTA 
AAAATGGTGA AGGAATTTGC TGAGGAAATG GAGAACATGC TGGGCAGAAA AATGAAATCA 
GTTAAGAGAA TGGCAGAGAC AGCAGAAGAC GCAGATTTTT ATCATGAATT TAATGCCACA 
TTAGAGTTTG ACTACTACAA TTCCATGTTG ATTAACACAT TAAATGAAGA TGGAAACAAC 
ATAGAGCTGG GGGGTGAATT TCCTTTGGAG GAGAATGAAC ACTTCAACAA TCTGCCTGTC 
AATACGCAGC AAAGCGTCGT ACAAGTGCCC ACAAATGTTT ATAACAAAGA TCCTGATATC 
CTGAACGGGG TGTTTATGTC AGAAGCACTG AATGACATAT TTATCAGTAA CTTTGAGAAG 
GACCCGACTC TAACCTGGCA GTACTTCGGC AGTTCAACTG GATTCTTTAG ACTTTACCCA 
GGTATTAAGT GGACACCTGA TGAAAATGGA GTTGTCACTT TTGATGGAAG GAATCGCAAC 
TGGTACATTC AAGCAGCCAC ATCTCCTAAA GACATCATTA TCGCTGTGGA CATCAGCGGG 
AGTATGAAGG GTTTGAGACT CACCATCGCA AAACACACCA TCAACACCAT TCTAGACACA 
CTAGGCGAAA ACGACTTTGT CAACGTCATT GCGTACAGTG ATTATGTCCA ATATGTAGAG 
CCCTGTTTTA AAGGCACACT GGTACAAGCT GATCTGGATA ACCGTGAGCA CTTTAAGCTT 
TTGGTGCAGG AGTTGCAGGT AAAAGGTGAA GGAAAGGTGA AGAAAGCCAT GAAAGAGTCC 
TTTAAGATTC TGAATGAGGC AGCAGCAGAA GGCAGGGGTA GTTTATGTAA TCAGGCTATC 
ATGCTGATCA CTGATGGAGC CATGGAGGAT TTCCAGCAGG TGTTTGAGGA GTTCAATTGG 
CCAGATCGCA AGGTGCGCGT GTTCACGTAT CTGATTGGAC GAGAGCTTAC ATTTGCCAGC 
AATGTCAAAT GGATTGCTTG CAATAATAAA GGTTACTACA CTCACATCTC TACACTGGCG 
GATGTACAGG AGAATGTGAT GGAATATCTT CATGTGCTGA GCAGACCAAT GGTCATCAAC 
CATGATCATG ACATCATTTG GACAGAGGCT TACATGGACA GTGTGCTTTT TAACACACAG 
GCCGAGAGTT TGCTGTTGAT GACCTCTGTA GCCATGCCTG TGTTCAGCAA GAAAGAAGAA 
ACACTCTCTC ATGGCATCCT CCTCGGTGTG GTTGGCACTG ATGTTCCCTT GAAGGAGCTC 
ATGAGATTGG CTCCTCGATA CAAGCTGGGG GTTCACGGCT ATGCATTCTT AAACACTAAT 
AATGGTTATA TACTCTCTCA TCCTGACCTT CGACCTCTGT ATAAAGATGG CAAGAAGCTG 
AAGCCAAAGC CGAACTATAA CAGTGTGGAT TTATCAGAGG TGGAGTGGGA GGATACAGAA 
GATGCACTGA GAACGGCGAT GGTAAAAGGG GAAACTGGGA CAATCTCTCT GGACGTCAGA 
GCAACAGTAG AAAAAGGAAA AAGAGTTGTG TTCCTGAAAA ATGAATATTT TTATACTACC 
ATCAATGAGA CGCCATTTAG TCTTGGGATT GTACTTACCA ACGGTCACGG GAAGAATGTT 
TTCATCGGAA ATGTTTCAGT TGAAGAAGGC CTACATGACC TGACTGCGTC TGATGTCAGT 
ATTGCTCAAG AATGGACATA CTGTGAGACA GACATCGATC CACATCATCG CAAATTGACG 
CAGCTTCAGG CCGTGGTTCG GTATTTAACA GGAAAAGAAC CAGATCTGGA ATGTGATGAA 
AGGCTTCTGC AGCAGACTCT GTTTGACGCA GTGGTCACAG CTCCTATGGA AGCCTATTGG 
ACTGCACTGC TGCTCAACGC CTCCGGTATT GATGGGGGAG TGGAGACAGC GTTCCTGGGC 
ACGCGCTCTG GCCTCATGAG GATGATCAGA TATGCTGGCG TAGAGAAGCG TGTGGCGAAA 
AAATTCTTAA CTGCATCAGA TAAGGAGAAT ATTTTTACTC TAGACCATTT CCCTGTTTGG 
TATCGGCGTG CGGCAGAAAA TCCAGCTGGC CGCTTTTTGT ACTACGTTCC TACAGAGGAC 
AACTCAGGAG TGAACGGCAG GTTTGTGATT GCTGTCACGC CTGTTACAGT CTCTGTGAGC 
AAAAAGACAG CAGTTGCTGG AGCCATCGGT GTACAGATGT CTCTTGAGAT GTTGGAAAAA 
AGGTTTTGGT CCATAGCCAA CCAGCCGAAC GACACAGATT GTTCAGGCAT AGAGGGGCTT 
TGTCCTTTAA GCTGTAGTGA TAATCCGGAC ATTAGCTGCT ACGTCATTGA CAGTAATGGC 
TTCATCATCA TCTCTAGGGA CAAGGCTGAT GTAGGCAAGT TTCTGGGGAA AATCGAAGGG 
TCAGTGATGT CCCAACTCTT GAAAATGGGC ATGTTCAAAA GAGTTGTACT CTATGACTAT 
CAGGCCATGT GTAAGGCGCA CGCTCACTCT GCTAGTGGCG CTCGACCCCT GCTAAGTCCC 
TTTTATAATC TTTTGGCAGC ACTGAAATGG TTCCTATCCA ACTTCCTGTT CCTTTTATTG 
GACTTCAACA TCTACAATCT GTGGCATTCG GATCACTTTA CAGAGGCCAA GACCTCATTC 
CATTCATCTA GTAAGAAGTT AAAGGGTGGC TTTCTGCAGC CGTGTGACAC TGAATATCCC 
AGCTTCGTCT ACGAGCCATC AATCAAAGAA ACCAACAGCC TGATCAAGTG TGGGAGATGT 
CAAAAGGTGT TCGTAATTCA ACAGATACCA GATAGCAACT TGCTGCTGGT GGTGATTCAG 
GCTGATTGTG ACTGTTCTCG GCAATATCCA CCCATCACCA TGGAGCCCAA AGAGGTCAAA 
TACAATGCCT CAGTGAAATG TGACCGCATG AGATCTCAGA AGATACGAAG AAGGCCTGAG 
TCTTGTCACG CTTACCACCC TCAAGAAAAT GCCAAGGACT GTGGTGGGGC TGTGGCAATC 
AGCCTGTCTA TCATGCTATT CTTTGCCTGT TTATCAGTGT CAGCACTCAT CCATCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CACNA2D4A ( XM_009300430.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009298705.1 Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 4a (cacna2d4a), transcript variant X1, mRNA.

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CloneID	ODa45370
Clone ID Related Accession (Same CDS sequence)	XM_009300430.2
Accession Version	XM_009300430.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3396bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent calcium channel subunit alpha-2/delta-4 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300430.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCAGG GGCTGATGGG TAGGATGAGG AGCGTCACAC TGCTCTGGGT GCTGTGTTCA 
AGCTGGACCA TCCAAACTCA AGCTCAGATG AGGATATCAC CTGAAACGGT TCAACAGTGG 
GCCAATAACT TTGGAAAACA GCTTATGGCC TTATCAAACA GATACTCAGG TGCTCAACTC 
TTACAAAAGA AATATAAAGA AGTGGAACCC ATCGTCAAGA TGGAGGAGAT CGATGGTTTA 
AAAATGGTGA AGGAATTTGC TGAGGAAATG GAGAACATGC TGGGCAGAAA AATGAAATCA 
GTTAAGAGAA TGGCAGAGAC AGCAGAAGAC GCAGATTTTT ATCATGAATT TAATGCCACA 
TTAGAGTTTG ACTACTACAA TTCCATGTTG ATTAACACAT TAAATGAAGA TGGAAACAAC 
ATAGAGCTGG GGGGTGAATT TCCTTTGGAG GAGAATGAAC ACTTCAACAA TCTGCCTGTC 
AATACGCAGC AAAGCGTCGT ACAAGTGCCC ACAAATGTTT ATAACAAAGA TCCTGATATC 
CTGAACGGGG TGTTTATGTC AGAAGCACTG AATGACATAT TTATCAGTAA CTTTGAGAAG 
GACCCGACTC TAACCTGGCA GTACTTCGGC AGTTCAACTG GATTCTTTAG ACTTTACCCA 
GGTATTAAGT GGACACCTGA TGAAAATGGA GTTGTCACTT TTGATGGAAG GAATCGCAAC 
TGGTACATTC AAGCAGCCAC ATCTCCTAAA GACATCATTA TCGCTGTGGA CATCAGCGGG 
AGTATGAAGG GTTTGAGACT CACCATCGCA AAACACACCA TCAACACCAT TCTAGACACA 
CTAGGCGAAA ACGACTTTGT CAACGTCATT GCGTACAGTG ATTATGTCCA ATATGTAGAG 
CCCTGTTTTA AAGGCACACT GGTACAAGCT GATCTGGATA ACCGTGAGCA CTTTAAGCTT 
TTGGTGCAGG AGTTGCAGGT AAAAGGTGAA GGAAAGGTGA AGAAAGCCAT GAAAGAGTCC 
TTTAAGATTC TGAATGAGGC AGCAGCAGAA GGCAGGGGTA GTTTATGTAA TCAGGCTATC 
ATGCTGATCA CTGATGGAGC CATGGAGGAT TTCCAGCAGG TGTTTGAGGA GTTCAATTGG 
CCAGATCGCA AGGTGCGCGT GTTCACGTAT CTGATTGGAC GAGAGCTTAC ATTTGCCAGC 
AATGTCAAAT GGATTGCTTG CAATAATAAA GGTTACTACA CTCACATCTC TACACTGGCG 
GATGTACAGG AGAATGTGAT GGAATATCTT CATGTGCTGA GCAGACCAAT GGTCATCAAC 
CATGATCATG ACATCATTTG GACAGAGGCT TACATGGACA GTGTGCTTTT TAACACACAG 
GCCGAGAGTT TGCTGTTGAT GACCTCTGTA GCCATGCCTG TGTTCAGCAA GAAAGAAGAA 
ACACTCTCTC ATGGCATCCT CCTCGGTGTG GTTGGCACTG ATGTTCCCTT GAAGGAGCTC 
ATGAGATTGG CTCCTCGATA CAAGCTGGGG GTTCACGGCT ATGCATTCTT AAACACTAAT 
AATGGTTATA TACTCTCTCA TCCTGACCTT CGACCTCTGT ATAAAGATGG CAAGAAGCTG 
AAGCCAAAGC CGAACTATAA CAGTGTGGAT TTATCAGAGG TGGAGTGGGA GGATACAGAA 
GATGCACTGA GAACGGCGAT GGTAAAAGGG GAAACTGGGA CAATCTCTCT GGACGTCAGA 
GCAACAGTAG AAAAAGGAAA AAGAGTTGTG TTCCTGAAAA ATGAATATTT TTATACTACC 
ATCAATGAGA CGCCATTTAG TCTTGGGATT GTACTTACCA ACGGTCACGG GAAGAATGTT 
TTCATCGGAA ATGTTTCAGT TGAAGAAGGC CTACATGACC TGACTGCGTC TGATGTCAGT 
ATTGCTCAAG AATGGACATA CTGTGAGACA GACATCGATC CACATCATCG CAAATTGACG 
CAGCTTCAGG CCGTGGTTCG GTATTTAACA GGAAAAGAAC CAGATCTGGA ATGTGATGAA 
AGGCTTCTGC AGCAGACTCT GTTTGACGCA GTGGTCACAG CTCCTATGGA AGCCTATTGG 
ACTGCACTGC TGCTCAACGC CTCCGGTATT GATGGGGGAG TGGAGACAGC GTTCCTGGGC 
ACGCGCTCTG GCCTCATGAG GATGATCAGA TATGCTGGCG TAGAGAAGCG TGTGGCGAAA 
AAATTCTTAA CTGCATCAGA TAAGGAGAAT ATTTTTACTC TAGACCATTT CCCTGTTTGG 
TATCGGCGTG CGGCAGAAAA TCCAGCTGGC CGCTTTTTGT ACTACGTTCC TACAGAGGAC 
AACTCAGGAG GTAGCCTGTA CCCTGCAGTC CACATCAAAA CAACTGAGAC TTTACAGCCT 
TTTGAGAGTA CAGTGCTCAG TATTTTTTTC GTTTCTTTCC CTGCAGTGAA CGGCAGGTTT 
GTGATTGCTG TCACGCCTGT TACAGTCTCT GTGAGCAAAA AGACAGCAGT TGCTGGAGCC 
ATCGGTGTAC AGATGTCTCT TGAGATGTTG GAAAAAAGGT TTTGGTCCAT AGCCAACCAG 
CCGAACGACA CAGATTGTTC AGGCATAGAG GGGCTTTGTC CTTTAAGCTG TAGTGATAAT 
CCGGACATTA GCTGCTACGT CATTGACAGT AATGGCTTCA TCATCATCTC TAGGGACAAG 
GCTGATGTAG GCAAGTTTCT GGGGAAAATC GAAGGGTCAG TGATGTCCCA ACTCTTGAAA 
ATGGGCATGT TCAAAAGAGT TGTACTCTAT GACTATCAGG CCATGTGTAA GGCGCACGCT 
CACTCTGCTA GTGGCGCTCG ACCCCTGCTA AGTCCCTTTT ATAATCTTTT GGCAGCACTG 
AAATGGTTCC TATCCAACTT CCTGTTCCTT TTATTGGACT TCAACATCTA CAATCTGTGG 
CATTCGGATC ACTTTACAGA GGCCAAGACC TCATTCCATT CATCTAGTAA GAAGTTAAAG 
GGTGGCTTTC TGCAGCCGTG TGACACTGAA TATCCCAGCT TCGTCTACGA GCCATCAATC 
AAAGAAACCA ACAGCCTGAT CAAGTGTGGG AGATGTCAAA AGGTGTTCGT AATTCAACAG 
ATACCAGATA GCAACTTGCT GCTGGTGGTG ATTCAGGCTG ATTGTGACTG TTCTCGGCAA 
TATCCACCCA TCACCATGGA GCCCAAAGAG GTCAAATACA ATGCCTCAGT GAAATGTGAC 
CGCATGAGAT CTCAGAAGAT ACGAAGAAGG CCTGAGTCTT GTCACGCTTA CCACCCTCAA 
GAAAATGCCA AGGACTGTGG TGGGGCTGTG GCAATCAGCC TGTCTATCAT GCTATTCTTT 
GCCTGTTTAT CAGTGTCAGC ACTCATCCAT CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298705.1
CDS	441..3836
Translation	MWQGLMGRMRSVTLLWVLCSSWTIQTQAQMRISPETVQQWANNFGKQLMALSNRYSGAQLLQKKYKEVEPIVKMEEIDGLKMVKEFAEEMENMLGRKMKSVKRMAETAEDADFYHEFNATLEFDYYNSMLINTLNEDGNNIELGGEFPLEENEHFNNLPVNTQQSVVQVPTNVYNKDPDILNGVFMSEALNDIFISNFEKDPTLTWQYFGSSTGFFRLYPGIKWTPDENGVVTFDGRNRNWYIQAATSPKDIIIAVDISGSMKGLRLTIAKHTINTILDTLGENDFVNVIAYSDYVQYVEPCFKGTLVQADLDNREHFKLLVQELQVKGEGKVKKAMKESFKILNEAAAEGRGSLCNQAIMLITDGAMEDFQQVFEEFNWPDRKVRVFTYLIGRELTFASNVKWIACNNKGYYTHISTLADVQENVMEYLHVLSRPMVINHDHDIIWTEAYMDSVLFNTQAESLLLMTSVAMPVFSKKEETLSHGILLGVVGTDVPLKELMRLAPRYKLGVHGYAFLNTNNGYILSHPDLRPLYKDGKKLKPKPNYNSVDLSEVEWEDTEDALRTAMVKGETGTISLDVRATVEKGKRVVFLKNEYFYTTINETPFSLGIVLTNGHGKNVFIGNVSVEEGLHDLTASDVSIAQEWTYCETDIDPHHRKLTQLQAVVRYLTGKEPDLECDERLLQQTLFDAVVTAPMEAYWTALLLNASGIDGGVETAFLGTRSGLMRMIRYAGVEKRVAKKFLTASDKENIFTLDHFPVWYRRAAENPAGRFLYYVPTEDNSGGSLYPAVHIKTTETLQPFESTVLSIFFVSFPAVNGRFVIAVTPVTVSVSKKTAVAGAIGVQMSLEMLEKRFWSIANQPNDTDCSGIEGLCPLSCSDNPDISCYVIDSNGFIIISRDKADVGKFLGKIEGSVMSQLLKMGMFKRVVLYDYQAMCKAHAHSASGARPLLSPFYNLLAALKWFLSNFLFLLLDFNIYNLWHSDHFTEAKTSFHSSSKKLKGGFLQPCDTEYPSFVYEPSIKETNSLIKCGRCQKVFVIQQIPDSNLLLVVIQADCDCSRQYPPITMEPKEVKYNASVKCDRMRSQKIRRRPESCHAYHPQENAKDCGGAVAISLSIMLFFACLSVSALIHR

Target ORF information:

RefSeq Version	XM_009300430.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage-dependent, alpha 2/delta subunit 4a (cacna2d4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300430.2

ORF Insert Sequence:

ATGTGGCAGG GGCTGATGGG TAGGATGAGG AGCGTCACAC TGCTCTGGGT GCTGTGTTCA 
AGCTGGACCA TCCAAACTCA AGCTCAGATG AGGATATCAC CTGAAACGGT TCAACAGTGG 
GCCAATAACT TTGGAAAACA GCTTATGGCC TTATCAAACA GATACTCAGG TGCTCAACTC 
TTACAAAAGA AATATAAAGA AGTGGAACCC ATCGTCAAGA TGGAGGAGAT CGATGGTTTA 
AAAATGGTGA AGGAATTTGC TGAGGAAATG GAGAACATGC TGGGCAGAAA AATGAAATCA 
GTTAAGAGAA TGGCAGAGAC AGCAGAAGAC GCAGATTTTT ATCATGAATT TAATGCCACA 
TTAGAGTTTG ACTACTACAA TTCCATGTTG ATTAACACAT TAAATGAAGA TGGAAACAAC 
ATAGAGCTGG GGGGTGAATT TCCTTTGGAG GAGAATGAAC ACTTCAACAA TCTGCCTGTC 
AATACGCAGC AAAGCGTCGT ACAAGTGCCC ACAAATGTTT ATAACAAAGA TCCTGATATC 
CTGAACGGGG TGTTTATGTC AGAAGCACTG AATGACATAT TTATCAGTAA CTTTGAGAAG 
GACCCGACTC TAACCTGGCA GTACTTCGGC AGTTCAACTG GATTCTTTAG ACTTTACCCA 
GGTATTAAGT GGACACCTGA TGAAAATGGA GTTGTCACTT TTGATGGAAG GAATCGCAAC 
TGGTACATTC AAGCAGCCAC ATCTCCTAAA GACATCATTA TCGCTGTGGA CATCAGCGGG 
AGTATGAAGG GTTTGAGACT CACCATCGCA AAACACACCA TCAACACCAT TCTAGACACA 
CTAGGCGAAA ACGACTTTGT CAACGTCATT GCGTACAGTG ATTATGTCCA ATATGTAGAG 
CCCTGTTTTA AAGGCACACT GGTACAAGCT GATCTGGATA ACCGTGAGCA CTTTAAGCTT 
TTGGTGCAGG AGTTGCAGGT AAAAGGTGAA GGAAAGGTGA AGAAAGCCAT GAAAGAGTCC 
TTTAAGATTC TGAATGAGGC AGCAGCAGAA GGCAGGGGTA GTTTATGTAA TCAGGCTATC 
ATGCTGATCA CTGATGGAGC CATGGAGGAT TTCCAGCAGG TGTTTGAGGA GTTCAATTGG 
CCAGATCGCA AGGTGCGCGT GTTCACGTAT CTGATTGGAC GAGAGCTTAC ATTTGCCAGC 
AATGTCAAAT GGATTGCTTG CAATAATAAA GGTTACTACA CTCACATCTC TACACTGGCG 
GATGTACAGG AGAATGTGAT GGAATATCTT CATGTGCTGA GCAGACCAAT GGTCATCAAC 
CATGATCATG ACATCATTTG GACAGAGGCT TACATGGACA GTGTGCTTTT TAACACACAG 
GCCGAGAGTT TGCTGTTGAT GACCTCTGTA GCCATGCCTG TGTTCAGCAA GAAAGAAGAA 
ACACTCTCTC ATGGCATCCT CCTCGGTGTG GTTGGCACTG ATGTTCCCTT GAAGGAGCTC 
ATGAGATTGG CTCCTCGATA CAAGCTGGGG GTTCACGGCT ATGCATTCTT AAACACTAAT 
AATGGTTATA TACTCTCTCA TCCTGACCTT CGACCTCTGT ATAAAGATGG CAAGAAGCTG 
AAGCCAAAGC CGAACTATAA CAGTGTGGAT TTATCAGAGG TGGAGTGGGA GGATACAGAA 
GATGCACTGA GAACGGCGAT GGTAAAAGGG GAAACTGGGA CAATCTCTCT GGACGTCAGA 
GCAACAGTAG AAAAAGGAAA AAGAGTTGTG TTCCTGAAAA ATGAATATTT TTATACTACC 
ATCAATGAGA CGCCATTTAG TCTTGGGATT GTACTTACCA ACGGTCACGG GAAGAATGTT 
TTCATCGGAA ATGTTTCAGT TGAAGAAGGC CTACATGACC TGACTGCGTC TGATGTCAGT 
ATTGCTCAAG AATGGACATA CTGTGAGACA GACATCGATC CACATCATCG CAAATTGACG 
CAGCTTCAGG CCGTGGTTCG GTATTTAACA GGAAAAGAAC CAGATCTGGA ATGTGATGAA 
AGGCTTCTGC AGCAGACTCT GTTTGACGCA GTGGTCACAG CTCCTATGGA AGCCTATTGG 
ACTGCACTGC TGCTCAACGC CTCCGGTATT GATGGGGGAG TGGAGACAGC GTTCCTGGGC 
ACGCGCTCTG GCCTCATGAG GATGATCAGA TATGCTGGCG TAGAGAAGCG TGTGGCGAAA 
AAATTCTTAA CTGCATCAGA TAAGGAGAAT ATTTTTACTC TAGACCATTT CCCTGTTTGG 
TATCGGCGTG CGGCAGAAAA TCCAGCTGGC CGCTTTTTGT ACTACGTTCC TACAGAGGAC 
AACTCAGGAG GTAGCCTGTA CCCTGCAGTC CACATCAAAA CAACTGAGAC TTTACAGCCT 
TTTGAGAGTA CAGTGCTCAG TATTTTTTTC GTTTCTTTCC CTGCAGTGAA CGGCAGGTTT 
GTGATTGCTG TCACGCCTGT TACAGTCTCT GTGAGCAAAA AGACAGCAGT TGCTGGAGCC 
ATCGGTGTAC AGATGTCTCT TGAGATGTTG GAAAAAAGGT TTTGGTCCAT AGCCAACCAG 
CCGAACGACA CAGATTGTTC AGGCATAGAG GGGCTTTGTC CTTTAAGCTG TAGTGATAAT 
CCGGACATTA GCTGCTACGT CATTGACAGT AATGGCTTCA TCATCATCTC TAGGGACAAG 
GCTGATGTAG GCAAGTTTCT GGGGAAAATC GAAGGGTCAG TGATGTCCCA ACTCTTGAAA 
ATGGGCATGT TCAAAAGAGT TGTACTCTAT GACTATCAGG CCATGTGTAA GGCGCACGCT 
CACTCTGCTA GTGGCGCTCG ACCCCTGCTA AGTCCCTTTT ATAATCTTTT GGCAGCACTG 
AAATGGTTCC TATCCAACTT CCTGTTCCTT TTATTGGACT TCAACATCTA CAATCTGTGG 
CATTCGGATC ACTTTACAGA GGCCAAGACC TCATTCCATT CATCTAGTAA GAAGTTAAAG 
GGTGGCTTTC TGCAGCCGTG TGACACTGAA TATCCCAGCT TCGTCTACGA GCCATCAATC 
AAAGAAACCA ACAGCCTGAT CAAGTGTGGG AGATGTCAAA AGGTGTTCGT AATTCAACAG 
ATACCAGATA GCAACTTGCT GCTGGTGGTG ATTCAGGCTG ATTGTGACTG TTCTCGGCAA 
TATCCACCCA TCACCATGGA GCCCAAAGAG GTCAAATACA ATGCCTCAGT GAAATGTGAC 
CGCATGAGAT CTCAGAAGAT ACGAAGAAGG CCTGAGTCTT GTCACGCTTA CCACCCTCAA 
GAAAATGCCA AGGACTGTGG TGGGGCTGTG GCAATCAGCC TGTCTATCAT GCTATTCTTT 
GCCTGTTTAT CAGTGTCAGC ACTCATCCAT CGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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CACNA2D4A cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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