dlc1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dlc1
Entrez Gene ID	567965
Full Name	DLC1 Rho GTPase activating protein
General protein information	Preferred Names DLC1 Rho GTPase activating protein Names DLC1 Rho GTPase activating protein rho GTPase-activating protein 7 unm_sa1141
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001921739.6	XP_001921774.4	rho GTPase-activating protein 7 isoform X1
XM_017355992.1	XP_017211481.1	rho GTPase-activating protein 7 isoform X1
XM_005159860.2	XP_005159917.1	rho GTPase-activating protein 7 isoform X2
XM_017355991.1	XP_017211480.1	rho GTPase-activating protein 7 isoform X1
XM_017355991.2	XP_017211480.1	rho GTPase-activating protein 7 isoform X1
XM_021476167.1	XP_021331842.1	rho GTPase-activating protein 7 isoform X2
XM_005159860.3	XP_005159917.1	rho GTPase-activating protein 7 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	DLC1	NP_872584.2
Pan troglodytes (chimpanzee)	DLC1	XP_001139210.2
Mus musculus (house mouse)	Dlc1	NP_001181869.1
Rattus norvegicus (Norway rat)	Dlc1	NP_001120918.1
Gallus gallus (chicken)	DLC1	XP_420693.3
	DLC1	XP_001092830.1
Canis lupus familiaris (dog)	DLC1	NP_001138543.1
Bos taurus (cattle)	DLC1	NP_001095963.1
Xenopus tropicalis (tropical clawed frog)	dlc1	XP_002934721.2
Danio rerio (zebrafish)	LOC567965	XP_005159917.1

Gene Ontology

The following dlc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dlc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa147386	XM_021476167.1 Latest version!	Danio rerio DLC1 Rho GTPase activating protein (dlc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa05571	XM_005159860.3 Latest version!	Danio rerio DLC1 Rho GTPase activating protein (dlc1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa53869	XM_001921739.6 Latest version!	Danio rerio DLC1 Rho GTPase activating protein (dlc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa53869	XM_017355992.1 Latest version!	Danio rerio DLC1 Rho GTPase activating protein (dlc1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa53869	XM_017355991.2 Latest version!	Danio rerio DLC1 Rho GTPase activating protein (dlc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
View Old Accession Versions >>
ODa53869	XM_017355991.1	Danio rerio DLC1 Rho GTPase activating protein (dlc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa05571	XM_005159860.2	Danio rerio DLC1 Rho GTPase activating protein (dlc1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dlc1 ( XM_021476167.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331842.1 Danio rerio DLC1 Rho GTPase activating protein (dlc1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa147386
Clone ID Related Accession (Same CDS sequence)	XM_021476167.1
Accession Version	XM_021476167.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho GTPase-activating protein 7 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACACTG CAGCACCGCA AGCTAAACCG GACGTAGAAA GCAGACTGAG GGAGGAGGAT 
GAGAATCTAG AAGCGACAGG GAAGATGTCG AGATCCATGC GTTTGCAGCT TCTTCAGAGA 
TCTCTGAGTG AACACATCAG ATCCTCCACA AGCAGAGCTC TGGATCTCTT AAATGGAAAC 
ATACAAGACC TCAGGCTGAC AGAAATTGAG GCGAAGGAGG CGTGTACATG GCTGAGGGCT 
GCAGGTTTTC CACAGTATGC TCAGCTTTAT GAAGATGGTC AGTTCCCGAT TGAGATTTCC 
TCAGTTACCA GAGATCACGA CTTCCTGGAT CAAGATGCCA TAGAAGCCTT GTGCAGGAGA 
CTGAACACCC TAAACAAGTG CGCTCTTATG AGATTAGAGA TCACCCCTCA AAGAAAGAGA 
AGTGAAGACT CTGATGAGGA TGAGCCATGT GCCATAAGTG GCCGTTGGAC TTTCCAGCGA 
GACAGCAAGC GCTGGTCTCG CATGGACCAG CTGGACCTTG ATGTATTCTC CCCTACTGCA 
GGGGACACTG GCTCCTTCGC TGCATTCCTA TCCCAAGATG AGCATTTTGT TAGAGGGCCC 
CTTCAAAAGG AGGGTGTTAA TGCCAGTGTG GAGAGCATCT TAACGGACCT TAGCGAGCAG 
CCAGAGGTGG GATCTCTGCA TAGTACTGGT AGTGGAGGTC GAGGAGGATC ACTGAGTACC 
ACTGTACCAT CCAGTCCTGC TACAACCATC TCCAATGCTA ATACAGCCAC CACAACAAGA 
GCAAGCTCAG TTGCCAGTGT CTGTTCTTCT GGAACATCAG GTGCCAATGA AGACTCCATG 
TCTGATGGGC TTCCATCGCC CCTGGAACGT GGTACCTTTG CTTTTGATGT AAAACCAAAC 
TTGATAAGTA GTGCAGCTGA AAAAAGCACA CGGTCAAGAG CAAAGAGCTT TTTAAAACGC 
ATGGAAAGTT TACGTCTACG CACCAGTAGC AATGCAGGCT CCAAGCGCAA GAAGAAAGGT 
GTTCCTGGGC ATGGTAGGTT GGAGATCAGT GGGCCAGTGC TTAAGGAGGG TCTTGATGAA 
GACAAGCTGC GTCGGCTCAA CTGTGTTGAC ATCGCAACTC TGGATCGAAA TGCAAACCGA 
AATCGAAGCA TCTCCTATTC AACGCAAACA AGCAGTGGGA GTGCAGGGAG TAGCCAATCA 
GAAGCTAGCA GTGGGAGTGC TGTAAGTACG CCCAGTCCAG TTTCAAGAGC ACGTAGTCAC 
AGCACAGCAG CCGGGTCCAG CAAGAGAGGT GGGATGTACT TGGAAGGTTT TGACCCATTT 
AACTTTGTTG TGCTGCAACC TTCTGAAGAC CAAGATGTAA AGGAGAACCA TCAGACCCAA 
AATAAGAGGA AGGAGCCCAG CTCAACTGAT GAGAACAATC GACGCAACCG TCAGAGTTCA 
GATAATAGGC AAATGGAGGA TGAAGAGAGT CGCGTAGAGG AAGGAGGTGT GATCTTTTTC 
TATTTGCCTG AAGGCCACAA GCCTGGCACT TTTCCTAAAG CACTTGCGGA TGGAGGATCC 
TATCATGGTG TTGACAACAC AAGTCGCCGC CCACCCCGGA GGGGCTCTTC TGCTTCACTA 
GACAGTCGAA TGAGTATCTA TGATAATGTT CCTTTCTCGG AAGTGGGAGA TGATGAGGAC 
GAAGTAGGAG AGGAGGAACC AAAACTGGAG GATGTGTTAG AGCGAGTCAG AGGTCTGCAG 
CAACTGGTCA ATGAATGGCC AGAAACAGGA ATTGGGGAAG AGGAGGAGGA TGAAGAGGAA 
GAGGAAGGAG ATTCAGATTC AGCCCTTGAC TCTGCATCTC CTTGTCCATC ATCTCCACTG 
CAGAACCGCT TAGAAGAGAC AGAGAATGGC AGCGACCAGG ACAGCACTGG AAACCCATTA 
GCAGAAAGAG ATGAGACACT CAGCTCACGT GAAAGATGTG ACTCTGGAGT TGAAGCATCA 
CTTACTCGAG CCAACAGAAC TCAGAAACTG CGCTGGCCAA GTTTCCAGAG CTCCCACAGG 
CCAAGTCTGT CATCAACAGT GCTACAAGTT GGATGTCAGT CAGTTCTGCA GATGAATCTA 
CTCCAGAAAT ACAGCCTTCT CAAACTTACT GCTCTACTGG AGAGATACAC GCCCACCAAC 
AAACATGGCT TCAGCTGGGC TGTTCCTAAG TTTATGAAGC GGATCAAGGT TCGCGACTAC 
AAGGACAGGA GTGTGTTTGG CGTTCCACTG CAGGTGATAG TTCAAAGGAG CGGACAACCC 
CTGCCTCAGA GCATTCAACA AGCCATGCGT TACCTGCGGA GCCAATGCCT CGACCAGGTG 
GGATTGTTCA GGAAATCAGG GGTGAAATCG CGAATCCAAG CCCTTCGCCA AATGAACGAA 
TCATGTGGCG CTGTTGGGGG AGGAGTCAAC TATGAGGGCC AATTAGCTTA TGACGTGGCT 
GACATGCTAA AGCAGTATTT CCGAGACCTT CCAGAGCCTC TGCTTACCAG CAAACTGTCT 
GATACCTTCC TGCAGATTTA TCAATATGTC CCCAAAGAGC AGCGCCTGCA GGCGGTAAGG 
GCTGCTGTGT TGCTGTTACC AGACGAGAAC CGCGAGGCAC TGCAGACTCT GCTGTGCTTC 
CTGAGTGATG TAACAGCCAG CGTGGGTGAA AACCAGATGA CCTGCACCAA CCTGGCCGTG 
TGTTTGGCCC CTTCTCTCTT CCACCTCAAC ACACTGCGCA GAGAGAGCTC GTCACCCAGG 
GTCATGAATA GAAAGAACAC CCTTGGAAAA CCTGACCAAA GAGACCTGAA TGAAAACCTG 
GCGGCTACTC ATGGTTTAGC GCATATGATA CAGGAGTGCA GAAAACTCTT CCAGATCCCA 
GAAGAGATGT CCCGATGTAG GAACTCTTAC ATGGAGCAGG GCTTGAGTCC CATGAGGATG 
GAGGTGTTGG CGGAAAACTG CGGTTCTTGC AACTACCAAA GTCTACTCAA AGACAGTATT 
GATAGCCTGC TTAAAGAAGC CAAGGACAAA TTCAAAGGTT ATGACAGCTG CTCGACTCCT 
GAAAACGCTG AGCTCGCATA CAAGAAGGTT CAGGACGGCT CTTCTCTGCG GCAGTGGAAA 
GTTTGTGTGG ATGTGCCTGC ATCGGCTGAG GATGTCCTGC AACGCATTTT GCGAGAGCAG 
GAGCGCTGGG ACGAGGACCT GCTAGAGTCC AGGATCGTGG AGACACTGGA CCACAACACT 
GAAGTCTATC AGTATGTCAG AAACTCAATG CCACCCCATC CGCCAAGAGA CCACGTGGTA 
CTCAGGTCAT GGATGACAGA TTTGCCCAAG GGAGTGTGTA CTTTAGTGAG CGTGTCAGTG 
GATCACGAGA GCGCAGCAGT GCTGGGTGTC CGTGCCAATG TTCTGACATC TCGCTATTAC 
ATCGAGCCCT GCGGAGCCAA CAAGAGCCGC CTCGCACACA TTTCCAGAAT AGACTGCAGG 
GGTCGTTGCC CTGAATGGTA CAATAAGCTA TATGGTCACC TATGTGCTGC GGAGGTGGTG 
CGGATACGAG ACACTTTTAC CTGTCTGGAC AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331842.1
CDS	27..3602
Translation	MHTAAPQAKPDVESRLREEDENLEATGKMSRSMRLQLLQRSLSEHIRSSTSRALDLLNGNIQDLRLTEIEAKEACTWLRAAGFPQYAQLYEDGQFPIEISSVTRDHDFLDQDAIEALCRRLNTLNKCALMRLEITPQRKRSEDSDEDEPCAISGRWTFQRDSKRWSRMDQLDLDVFSPTAGDTGSFAAFLSQDEHFVRGPLQKEGVNASVESILTDLSEQPEVGSLHSTGSGGRGGSLSTTVPSSPATTISNANTATTTRASSVASVCSSGTSGANEDSMSDGLPSPLERGTFAFDVKPNLISSAAEKSTRSRAKSFLKRMESLRLRTSSNAGSKRKKKGVPGHGRLEISGPVLKEGLDEDKLRRLNCVDIATLDRNANRNRSISYSTQTSSGSAGSSQSEASSGSAVSTPSPVSRARSHSTAAGSSKRGGMYLEGFDPFNFVVLQPSEDQDVKENHQTQNKRKEPSSTDENNRRNRQSSDNRQMEDEESRVEEGGVIFFYLPEGHKPGTFPKALADGGSYHGVDNTSRRPPRRGSSASLDSRMSIYDNVPFSEVGDDEDEVGEEEPKLEDVLERVRGLQQLVNEWPETGIGEEEEDEEEEEGDSDSALDSASPCPSSPLQNRLEETENGSDQDSTGNPLAERDETLSSRERCDSGVEASLTRANRTQKLRWPSFQSSHRPSLSSTVLQVGCQSVLQMNLLQKYSLLKLTALLERYTPTNKHGFSWAVPKFMKRIKVRDYKDRSVFGVPLQVIVQRSGQPLPQSIQQAMRYLRSQCLDQVGLFRKSGVKSRIQALRQMNESCGAVGGGVNYEGQLAYDVADMLKQYFRDLPEPLLTSKLSDTFLQIYQYVPKEQRLQAVRAAVLLLPDENREALQTLLCFLSDVTASVGENQMTCTNLAVCLAPSLFHLNTLRRESSSPRVMNRKNTLGKPDQRDLNENLAATHGLAHMIQECRKLFQIPEEMSRCRNSYMEQGLSPMRMEVLAENCGSCNYQSLLKDSIDSLLKEAKDKFKGYDSCSTPENAELAYKKVQDGSSLRQWKVCVDVPASAEDVLQRILREQERWDEDLLESRIVETLDHNTEVYQYVRNSMPPHPPRDHVVLRSWMTDLPKGVCTLVSVSVDHESAAVLGVRANVLTSRYYIEPCGANKSRLAHISRIDCRGRCPEWYNKLYGHLCAAEVVRIRDTFTCLDK

Target ORF information:

RefSeq Version	XM_021476167.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DLC1 Rho GTPase activating protein (dlc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476167.1

ORF Insert Sequence:

ATGCACACTG CAGCACCGCA AGCTAAACCG GACGTAGAAA GCAGACTGAG GGAGGAGGAT 
GAGAATCTAG AAGCGACAGG GAAGATGTCG AGATCCATGC GTTTGCAGCT TCTTCAGAGA 
TCTCTGAGTG AACACATCAG ATCCTCCACA AGCAGAGCTC TGGATCTCTT AAATGGAAAC 
ATACAAGACC TCAGGCTGAC AGAAATTGAG GCGAAGGAGG CGTGTACATG GCTGAGGGCT 
GCAGGTTTTC CACAGTATGC TCAGCTTTAT GAAGATGGTC AGTTCCCGAT TGAGATTTCC 
TCAGTTACCA GAGATCACGA CTTCCTGGAT CAAGATGCCA TAGAAGCCTT GTGCAGGAGA 
CTGAACACCC TAAACAAGTG CGCTCTTATG AGATTAGAGA TCACCCCTCA AAGAAAGAGA 
AGTGAAGACT CTGATGAGGA TGAGCCATGT GCCATAAGTG GCCGTTGGAC TTTCCAGCGA 
GACAGCAAGC GCTGGTCTCG CATGGACCAG CTGGACCTTG ATGTATTCTC CCCTACTGCA 
GGGGACACTG GCTCCTTCGC TGCATTCCTA TCCCAAGATG AGCATTTTGT TAGAGGGCCC 
CTTCAAAAGG AGGGTGTTAA TGCCAGTGTG GAGAGCATCT TAACGGACCT TAGCGAGCAG 
CCAGAGGTGG GATCTCTGCA TAGTACTGGT AGTGGAGGTC GAGGAGGATC ACTGAGTACC 
ACTGTACCAT CCAGTCCTGC TACAACCATC TCCAATGCTA ATACAGCCAC CACAACAAGA 
GCAAGCTCAG TTGCCAGTGT CTGTTCTTCT GGAACATCAG GTGCCAATGA AGACTCCATG 
TCTGATGGGC TTCCATCGCC CCTGGAACGT GGTACCTTTG CTTTTGATGT AAAACCAAAC 
TTGATAAGTA GTGCAGCTGA AAAAAGCACA CGGTCAAGAG CAAAGAGCTT TTTAAAACGC 
ATGGAAAGTT TACGTCTACG CACCAGTAGC AATGCAGGCT CCAAGCGCAA GAAGAAAGGT 
GTTCCTGGGC ATGGTAGGTT GGAGATCAGT GGGCCAGTGC TTAAGGAGGG TCTTGATGAA 
GACAAGCTGC GTCGGCTCAA CTGTGTTGAC ATCGCAACTC TGGATCGAAA TGCAAACCGA 
AATCGAAGCA TCTCCTATTC AACGCAAACA AGCAGTGGGA GTGCAGGGAG TAGCCAATCA 
GAAGCTAGCA GTGGGAGTGC TGTAAGTACG CCCAGTCCAG TTTCAAGAGC ACGTAGTCAC 
AGCACAGCAG CCGGGTCCAG CAAGAGAGGT GGGATGTACT TGGAAGGTTT TGACCCATTT 
AACTTTGTTG TGCTGCAACC TTCTGAAGAC CAAGATGTAA AGGAGAACCA TCAGACCCAA 
AATAAGAGGA AGGAGCCCAG CTCAACTGAT GAGAACAATC GACGCAACCG TCAGAGTTCA 
GATAATAGGC AAATGGAGGA TGAAGAGAGT CGCGTAGAGG AAGGAGGTGT GATCTTTTTC 
TATTTGCCTG AAGGCCACAA GCCTGGCACT TTTCCTAAAG CACTTGCGGA TGGAGGATCC 
TATCATGGTG TTGACAACAC AAGTCGCCGC CCACCCCGGA GGGGCTCTTC TGCTTCACTA 
GACAGTCGAA TGAGTATCTA TGATAATGTT CCTTTCTCGG AAGTGGGAGA TGATGAGGAC 
GAAGTAGGAG AGGAGGAACC AAAACTGGAG GATGTGTTAG AGCGAGTCAG AGGTCTGCAG 
CAACTGGTCA ATGAATGGCC AGAAACAGGA ATTGGGGAAG AGGAGGAGGA TGAAGAGGAA 
GAGGAAGGAG ATTCAGATTC AGCCCTTGAC TCTGCATCTC CTTGTCCATC ATCTCCACTG 
CAGAACCGCT TAGAAGAGAC AGAGAATGGC AGCGACCAGG ACAGCACTGG AAACCCATTA 
GCAGAAAGAG ATGAGACACT CAGCTCACGT GAAAGATGTG ACTCTGGAGT TGAAGCATCA 
CTTACTCGAG CCAACAGAAC TCAGAAACTG CGCTGGCCAA GTTTCCAGAG CTCCCACAGG 
CCAAGTCTGT CATCAACAGT GCTACAAGTT GGATGTCAGT CAGTTCTGCA GATGAATCTA 
CTCCAGAAAT ACAGCCTTCT CAAACTTACT GCTCTACTGG AGAGATACAC GCCCACCAAC 
AAACATGGCT TCAGCTGGGC TGTTCCTAAG TTTATGAAGC GGATCAAGGT TCGCGACTAC 
AAGGACAGGA GTGTGTTTGG CGTTCCACTG CAGGTGATAG TTCAAAGGAG CGGACAACCC 
CTGCCTCAGA GCATTCAACA AGCCATGCGT TACCTGCGGA GCCAATGCCT CGACCAGGTG 
GGATTGTTCA GGAAATCAGG GGTGAAATCG CGAATCCAAG CCCTTCGCCA AATGAACGAA 
TCATGTGGCG CTGTTGGGGG AGGAGTCAAC TATGAGGGCC AATTAGCTTA TGACGTGGCT 
GACATGCTAA AGCAGTATTT CCGAGACCTT CCAGAGCCTC TGCTTACCAG CAAACTGTCT 
GATACCTTCC TGCAGATTTA TCAATATGTC CCCAAAGAGC AGCGCCTGCA GGCGGTAAGG 
GCTGCTGTGT TGCTGTTACC AGACGAGAAC CGCGAGGCAC TGCAGACTCT GCTGTGCTTC 
CTGAGTGATG TAACAGCCAG CGTGGGTGAA AACCAGATGA CCTGCACCAA CCTGGCCGTG 
TGTTTGGCCC CTTCTCTCTT CCACCTCAAC ACACTGCGCA GAGAGAGCTC GTCACCCAGG 
GTCATGAATA GAAAGAACAC CCTTGGAAAA CCTGACCAAA GAGACCTGAA TGAAAACCTG 
GCGGCTACTC ATGGTTTAGC GCATATGATA CAGGAGTGCA GAAAACTCTT CCAGATCCCA 
GAAGAGATGT CCCGATGTAG GAACTCTTAC ATGGAGCAGG GCTTGAGTCC CATGAGGATG 
GAGGTGTTGG CGGAAAACTG CGGTTCTTGC AACTACCAAA GTCTACTCAA AGACAGTATT 
GATAGCCTGC TTAAAGAAGC CAAGGACAAA TTCAAAGGTT ATGACAGCTG CTCGACTCCT 
GAAAACGCTG AGCTCGCATA CAAGAAGGTT CAGGACGGCT CTTCTCTGCG GCAGTGGAAA 
GTTTGTGTGG ATGTGCCTGC ATCGGCTGAG GATGTCCTGC AACGCATTTT GCGAGAGCAG 
GAGCGCTGGG ACGAGGACCT GCTAGAGTCC AGGATCGTGG AGACACTGGA CCACAACACT 
GAAGTCTATC AGTATGTCAG AAACTCAATG CCACCCCATC CGCCAAGAGA CCACGTGGTA 
CTCAGGTCAT GGATGACAGA TTTGCCCAAG GGAGTGTGTA CTTTAGTGAG CGTGTCAGTG 
GATCACGAGA GCGCAGCAGT GCTGGGTGTC CGTGCCAATG TTCTGACATC TCGCTATTAC 
ATCGAGCCCT GCGGAGCCAA CAAGAGCCGC CTCGCACACA TTTCCAGAAT AGACTGCAGG 
GGTCGTTGCC CTGAATGGTA CAATAAGCTA TATGGTCACC TATGTGCTGC GGAGGTGGTG 
CGGATACGAG ACACTTTTAC CTGTCTGGAC AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dlc1 ( XM_005159860.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159917.1 Danio rerio DLC1 Rho GTPase activating protein (dlc1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa05571
Clone ID Related Accession (Same CDS sequence)	XM_005159860.2 , XM_005159860.3
Accession Version	XM_005159860.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho GTPase-activating protein 7 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005159860.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCTTA CTCAAATTGA GGCGAAGGAG GCGTGTACAT GGCTGAGGGC TGCAGGTTTT 
CCACAGTATG CTCAGCTTTA TGAAGATGGT CAGTTCCCGA TTGAGATTTC CTCAGTTACC 
AGAGATCACG ACTTCCTGGA TCAAGATGCC ATAGAAGCCT TGTGCAGGAG ACTGAACACC 
CTAAACAAGT GCGCTCTTAT GAGATTAGAG ATCACCCCTC AAAGAAAGAG AAGTGAAGAC 
TCTGATGAGG ATGAGCCATG TGCCATAAGT GGCCGTTGGA CTTTCCAGCG AGACAGCAAG 
CGCTGGTCTC GCATGGACCA GCTGGACCTT GATGTATTCT CCCCTACTGC AGGGGACACT 
GGCTCCTTCG CTGCATTCCT ATCCCAAGAT GAGCATTTTG TTAGAGGGCC CCTTCAAAAG 
GAGGGTGTTA ATGCCAGTGT GGAGAGCATC TTAACGGACC TTAGCGAGCA GCCAGAGGTG 
GGATCTCTGC ATAGTACTGG TAGTGGAGGT CGAGGAGGAT CACTGAGTAC CACTGTACCA 
TCCAGTCCTG CTACAACCAT CTCCAATGCT AATACAGCCA CCACAACAAG AGCAAGCTCA 
GTTGCCAGTG TCTGTTCTTC TGGAACATCA GGTGCCAATG AAGACTCCAT GTCTGATGGG 
CTTCCATCGC CCCTGGAACG TGGTACCTTT GCTTTTGATG TAAAACCAAA CTTGATAAGT 
AGTGCAGCTG AAAAAAGCAC ACGGTCAAGA GCAAAGAGCT TTTTAAAACG CATGGAAAGT 
TTACGTCTAC GCACCAGTAG CAATGCAGGC TCCAAGCGCA AGAAGAAAGG TGTTCCTGGG 
CATGGTAGGT TGGAGATCAG TGGGCCAGTG CTTAAGGAGG GTCTTGATGA AGACAAGCTG 
CGTCGGCTCA ACTGTGTTGA CATCGCAACT CTGGATCGAA ATGCAAACCG AAATCGAAGC 
ATCTCCTATT CAACGCAAAC AAGCAGTGGG AGTGCAGGGA GTAGCCAATC AGAAGCTAGC 
AGTGGGAGTG CTGTAAGTAC GCCCAGTCCA GTTTCAAGAG CACGTAGTCA CAGCACAGCA 
GCCGGGTCCA GCAAGAGAGG TGGGATGTAC TTGGAAGGTT TTGACCCATT TAACTTTGTT 
GTGCTGCAAC CTTCTGAAGA CCAAGATGTA AAGGAGAACC ATCAGACCCA AAATAAGAGG 
AAGGAGCCCA GCTCAACTGA TGAGAACAAT CGACGCAACC GTCAGAGTTC AGATAATAGG 
CAAATGGAGG ATGAAGAGAG TCGCGTAGAG GAAGGAGGTG TGATCTTTTT CTATTTGCCT 
GAAGGCCACA AGCCTGGCAC TTTTCCTAAA GCACTTGCGG ATGGAGGATC CTATCATGGT 
GTTGACAACA CAAGTCGCCG CCCACCCCGG AGGGGCTCTT CTGCTTCACT AGACAGTCGA 
ATGAGTATCT ATGATAATGT TCCTTTCTCG GAAGTGGGAG ATGATGAGGA CGAAGTAGGA 
GAGGAGGAAC CAAAACTGGA GGATGTGTTA GAGCGAGTCA GAGGTCTGCA GCAACTGGTC 
AATGAATGGC CAGAAACAGG AATTGGGGAA GAGGAGGAGG ATGAAGAGGA AGAGGAAGGA 
GATTCAGATT CAGCCCTTGA CTCTGCATCT CCTTGTCCAT CATCTCCACT GCAGAACCGC 
TTAGAAGAGA CAGAGAATGG CAGCGACCAG GACAGCACTG GAAACCCATT AGCAGAAAGA 
GATGAGACAC TCAGCTCACG TGAAAGATGT GACTCTGGAG TTGAAGCATC ACTTACTCGA 
GCCAACAGAA CTCAGAAACT GCGCTGGCCA AGTTTCCAGA GCTCCCACAG GCCAAGTCTG 
TCATCAACAG TGCTACAAGT TGGATGTCAG TCAGTTCTGC AGATGAATCT ACTCCAGAAA 
TACAGCCTTC TCAAACTTAC TGCTCTACTG GAGAGATACA CGCCCACCAA CAAACATGGC 
TTCAGCTGGG CTGTTCCTAA GTTTATGAAG CGGATCAAGG TTCGCGACTA CAAGGACAGG 
AGTGTGTTTG GCGTTCCACT GCAGGTGATA GTTCAAAGGA GCGGACAACC CCTGCCTCAG 
AGCATTCAAC AAGCCATGCG TTACCTGCGG AGCCAATGCC TCGACCAGGT GGGATTGTTC 
AGGAAATCAG GGGTGAAATC GCGAATCCAA GCCCTTCGCC AAATGAACGA ATCATGTGGC 
GCTGTTGGGG GAGGAGTCAA CTATGAGGGC CAATTAGCTT ATGACGTGGC TGACATGCTA 
AAGCAGTATT TCCGAGACCT TCCAGAGCCT CTGCTTACCA GCAAACTGTC TGATACCTTC 
CTGCAGATTT ATCAATATGT CCCCAAAGAG CAGCGCCTGC AGGCGGTAAG GGCTGCTGTG 
TTGCTGTTAC CAGACGAGAA CCGCGAGGCA CTGCAGACTC TGCTGTGCTT CCTGAGTGAT 
GTAACAGCCA GCGTGGGTGA AAACCAGATG ACCTGCACCA ACCTGGCCGT GTGTTTGGCC 
CCTTCTCTCT TCCACCTCAA CACACTGCGC AGAGAGAGCT CGTCACCCAG GGTCATGAAT 
AGAAAGAACA CCCTTGGAAA ACCTGACCAA AGAGACCTGA ATGAAAACCT GGCGGCTACT 
CATGGTTTAG CGCATATGAT ACAGGAGTGC AGAAAACTCT TCCAGATCCC AGAAGAGATG 
TCCCGATGTA GGAACTCTTA CATGGAGCAG GGCTTGAGTC CCATGAGGAT GGAGGTGTTG 
GCGGAAAACT GCGGTTCTTG CAACTACCAA AGTCTACTCA AAGACAGTAT TGATAGCCTG 
CTTAAAGAAG CCAAGGACAA ATTCAAAGGT TATGACAGCT GCTCGACTCC TGAAAACGCT 
GAGCTCGCAT ACAAGAAGGT TCAGGACGGC TCTTCTCTGC GGCAGTGGAA AGTTTGTGTG 
GATGTGCCTG CATCGGCTGA GGATGTCCTG CAACGCATTT TGCGAGAGCA GGAGCGCTGG 
GACGAGGACC TGCTAGAGTC CAGGATCGTG GAGACACTGG ACCACAACAC TGAAGTCTAT 
CAGTATGTCA GAAACTCAAT GCCACCCCAT CCGCCAAGAG ACCACGTGGT ACTCAGGTCA 
TGGATGACAG ATTTGCCCAA GGGAGTGTGT ACTTTAGTGA GCGTGTCAGT GGATCACGAG 
AGCGCAGCAG TGCTGGGTGT CCGTGCCAAT GTTCTGACAT CTCGCTATTA CATCGAGCCC 
TGCGGAGCCA ACAAGAGCCG CCTCGCACAC ATTTCCAGAA TAGACTGCAG GGGTCGTTGC 
CCTGAATGGT ACAATAAGCT ATATGGTCAC CTATGTGCTG CGGAGGTGGT GCGGATACGA 
GACACTTTTA CCTGTCTGGA CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159917.1
CDS	284..3670
Translation	MILTQIEAKEACTWLRAAGFPQYAQLYEDGQFPIEISSVTRDHDFLDQDAIEALCRRLNTLNKCALMRLEITPQRKRSEDSDEDEPCAISGRWTFQRDSKRWSRMDQLDLDVFSPTAGDTGSFAAFLSQDEHFVRGPLQKEGVNASVESILTDLSEQPEVGSLHSTGSGGRGGSLSTTVPSSPATTISNANTATTTRASSVASVCSSGTSGANEDSMSDGLPSPLERGTFAFDVKPNLISSAAEKSTRSRAKSFLKRMESLRLRTSSNAGSKRKKKGVPGHGRLEISGPVLKEGLDEDKLRRLNCVDIATLDRNANRNRSISYSTQTSSGSAGSSQSEASSGSAVSTPSPVSRARSHSTAAGSSKRGGMYLEGFDPFNFVVLQPSEDQDVKENHQTQNKRKEPSSTDENNRRNRQSSDNRQMEDEESRVEEGGVIFFYLPEGHKPGTFPKALADGGSYHGVDNTSRRPPRRGSSASLDSRMSIYDNVPFSEVGDDEDEVGEEEPKLEDVLERVRGLQQLVNEWPETGIGEEEEDEEEEEGDSDSALDSASPCPSSPLQNRLEETENGSDQDSTGNPLAERDETLSSRERCDSGVEASLTRANRTQKLRWPSFQSSHRPSLSSTVLQVGCQSVLQMNLLQKYSLLKLTALLERYTPTNKHGFSWAVPKFMKRIKVRDYKDRSVFGVPLQVIVQRSGQPLPQSIQQAMRYLRSQCLDQVGLFRKSGVKSRIQALRQMNESCGAVGGGVNYEGQLAYDVADMLKQYFRDLPEPLLTSKLSDTFLQIYQYVPKEQRLQAVRAAVLLLPDENREALQTLLCFLSDVTASVGENQMTCTNLAVCLAPSLFHLNTLRRESSSPRVMNRKNTLGKPDQRDLNENLAATHGLAHMIQECRKLFQIPEEMSRCRNSYMEQGLSPMRMEVLAENCGSCNYQSLLKDSIDSLLKEAKDKFKGYDSCSTPENAELAYKKVQDGSSLRQWKVCVDVPASAEDVLQRILREQERWDEDLLESRIVETLDHNTEVYQYVRNSMPPHPPRDHVVLRSWMTDLPKGVCTLVSVSVDHESAAVLGVRANVLTSRYYIEPCGANKSRLAHISRIDCRGRCPEWYNKLYGHLCAAEVVRIRDTFTCLDK

Target ORF information:

RefSeq Version	XM_005159860.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DLC1 Rho GTPase activating protein (dlc1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159860.2

ORF Insert Sequence:

ATGATCCTTA CTCAAATTGA GGCGAAGGAG GCGTGTACAT GGCTGAGGGC TGCAGGTTTT 
CCACAGTATG CTCAGCTTTA TGAAGATGGT CAGTTCCCGA TTGAGATTTC CTCAGTTACC 
AGAGATCACG ACTTCCTGGA TCAAGATGCC ATAGAAGCCT TGTGCAGGAG ACTGAACACC 
CTAAACAAGT GCGCTCTTAT GAGATTAGAG ATCACCCCTC AAAGAAAGAG AAGTGAAGAC 
TCTGATGAGG ATGAGCCATG TGCCATAAGT GGCCGTTGGA CTTTCCAGCG AGACAGCAAG 
CGCTGGTCTC GCATGGACCA GCTGGACCTT GATGTATTCT CCCCTACTGC AGGGGACACT 
GGCTCCTTCG CTGCATTCCT ATCCCAAGAT GAGCATTTTG TTAGAGGGCC CCTTCAAAAG 
GAGGGTGTTA ATGCCAGTGT GGAGAGCATC TTAACGGACC TTAGCGAGCA GCCAGAGGTG 
GGATCTCTGC ATAGTACTGG TAGTGGAGGT CGAGGAGGAT CACTGAGTAC CACTGTACCA 
TCCAGTCCTG CTACAACCAT CTCCAATGCT AATACAGCCA CCACAACAAG AGCAAGCTCA 
GTTGCCAGTG TCTGTTCTTC TGGAACATCA GGTGCCAATG AAGACTCCAT GTCTGATGGG 
CTTCCATCGC CCCTGGAACG TGGTACCTTT GCTTTTGATG TAAAACCAAA CTTGATAAGT 
AGTGCAGCTG AAAAAAGCAC ACGGTCAAGA GCAAAGAGCT TTTTAAAACG CATGGAAAGT 
TTACGTCTAC GCACCAGTAG CAATGCAGGC TCCAAGCGCA AGAAGAAAGG TGTTCCTGGG 
CATGGTAGGT TGGAGATCAG TGGGCCAGTG CTTAAGGAGG GTCTTGATGA AGACAAGCTG 
CGTCGGCTCA ACTGTGTTGA CATCGCAACT CTGGATCGAA ATGCAAACCG AAATCGAAGC 
ATCTCCTATT CAACGCAAAC AAGCAGTGGG AGTGCAGGGA GTAGCCAATC AGAAGCTAGC 
AGTGGGAGTG CTGTAAGTAC GCCCAGTCCA GTTTCAAGAG CACGTAGTCA CAGCACAGCA 
GCCGGGTCCA GCAAGAGAGG TGGGATGTAC TTGGAAGGTT TTGACCCATT TAACTTTGTT 
GTGCTGCAAC CTTCTGAAGA CCAAGATGTA AAGGAGAACC ATCAGACCCA AAATAAGAGG 
AAGGAGCCCA GCTCAACTGA TGAGAACAAT CGACGCAACC GTCAGAGTTC AGATAATAGG 
CAAATGGAGG ATGAAGAGAG TCGCGTAGAG GAAGGAGGTG TGATCTTTTT CTATTTGCCT 
GAAGGCCACA AGCCTGGCAC TTTTCCTAAA GCACTTGCGG ATGGAGGATC CTATCATGGT 
GTTGACAACA CAAGTCGCCG CCCACCCCGG AGGGGCTCTT CTGCTTCACT AGACAGTCGA 
ATGAGTATCT ATGATAATGT TCCTTTCTCG GAAGTGGGAG ATGATGAGGA CGAAGTAGGA 
GAGGAGGAAC CAAAACTGGA GGATGTGTTA GAGCGAGTCA GAGGTCTGCA GCAACTGGTC 
AATGAATGGC CAGAAACAGG AATTGGGGAA GAGGAGGAGG ATGAAGAGGA AGAGGAAGGA 
GATTCAGATT CAGCCCTTGA CTCTGCATCT CCTTGTCCAT CATCTCCACT GCAGAACCGC 
TTAGAAGAGA CAGAGAATGG CAGCGACCAG GACAGCACTG GAAACCCATT AGCAGAAAGA 
GATGAGACAC TCAGCTCACG TGAAAGATGT GACTCTGGAG TTGAAGCATC ACTTACTCGA 
GCCAACAGAA CTCAGAAACT GCGCTGGCCA AGTTTCCAGA GCTCCCACAG GCCAAGTCTG 
TCATCAACAG TGCTACAAGT TGGATGTCAG TCAGTTCTGC AGATGAATCT ACTCCAGAAA 
TACAGCCTTC TCAAACTTAC TGCTCTACTG GAGAGATACA CGCCCACCAA CAAACATGGC 
TTCAGCTGGG CTGTTCCTAA GTTTATGAAG CGGATCAAGG TTCGCGACTA CAAGGACAGG 
AGTGTGTTTG GCGTTCCACT GCAGGTGATA GTTCAAAGGA GCGGACAACC CCTGCCTCAG 
AGCATTCAAC AAGCCATGCG TTACCTGCGG AGCCAATGCC TCGACCAGGT GGGATTGTTC 
AGGAAATCAG GGGTGAAATC GCGAATCCAA GCCCTTCGCC AAATGAACGA ATCATGTGGC 
GCTGTTGGGG GAGGAGTCAA CTATGAGGGC CAATTAGCTT ATGACGTGGC TGACATGCTA 
AAGCAGTATT TCCGAGACCT TCCAGAGCCT CTGCTTACCA GCAAACTGTC TGATACCTTC 
CTGCAGATTT ATCAATATGT CCCCAAAGAG CAGCGCCTGC AGGCGGTAAG GGCTGCTGTG 
TTGCTGTTAC CAGACGAGAA CCGCGAGGCA CTGCAGACTC TGCTGTGCTT CCTGAGTGAT 
GTAACAGCCA GCGTGGGTGA AAACCAGATG ACCTGCACCA ACCTGGCCGT GTGTTTGGCC 
CCTTCTCTCT TCCACCTCAA CACACTGCGC AGAGAGAGCT CGTCACCCAG GGTCATGAAT 
AGAAAGAACA CCCTTGGAAA ACCTGACCAA AGAGACCTGA ATGAAAACCT GGCGGCTACT 
CATGGTTTAG CGCATATGAT ACAGGAGTGC AGAAAACTCT TCCAGATCCC AGAAGAGATG 
TCCCGATGTA GGAACTCTTA CATGGAGCAG GGCTTGAGTC CCATGAGGAT GGAGGTGTTG 
GCGGAAAACT GCGGTTCTTG CAACTACCAA AGTCTACTCA AAGACAGTAT TGATAGCCTG 
CTTAAAGAAG CCAAGGACAA ATTCAAAGGT TATGACAGCT GCTCGACTCC TGAAAACGCT 
GAGCTCGCAT ACAAGAAGGT TCAGGACGGC TCTTCTCTGC GGCAGTGGAA AGTTTGTGTG 
GATGTGCCTG CATCGGCTGA GGATGTCCTG CAACGCATTT TGCGAGAGCA GGAGCGCTGG 
GACGAGGACC TGCTAGAGTC CAGGATCGTG GAGACACTGG ACCACAACAC TGAAGTCTAT 
CAGTATGTCA GAAACTCAAT GCCACCCCAT CCGCCAAGAG ACCACGTGGT ACTCAGGTCA 
TGGATGACAG ATTTGCCCAA GGGAGTGTGT ACTTTAGTGA GCGTGTCAGT GGATCACGAG 
AGCGCAGCAG TGCTGGGTGT CCGTGCCAAT GTTCTGACAT CTCGCTATTA CATCGAGCCC 
TGCGGAGCCA ACAAGAGCCG CCTCGCACAC ATTTCCAGAA TAGACTGCAG GGGTCGTTGC 
CCTGAATGGT ACAATAAGCT ATATGGTCAC CTATGTGCTG CGGAGGTGGT GCGGATACGA 
GACACTTTTA CCTGTCTGGA CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dlc1 ( XM_005159860.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159917.1 Danio rerio DLC1 Rho GTPase activating protein (dlc1), transcript variant X3, mRNA.

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CloneID	ODa05571
Clone ID Related Accession (Same CDS sequence)	XM_005159860.2 , XM_005159860.3
Accession Version	XM_005159860.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3387bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho GTPase-activating protein 7 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159860.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCCTTA CTCAAATTGA GGCGAAGGAG GCGTGTACAT GGCTGAGGGC TGCAGGTTTT 
CCACAGTATG CTCAGCTTTA TGAAGATGGT CAGTTCCCGA TTGAGATTTC CTCAGTTACC 
AGAGATCACG ACTTCCTGGA TCAAGATGCC ATAGAAGCCT TGTGCAGGAG ACTGAACACC 
CTAAACAAGT GCGCTCTTAT GAGATTAGAG ATCACCCCTC AAAGAAAGAG AAGTGAAGAC 
TCTGATGAGG ATGAGCCATG TGCCATAAGT GGCCGTTGGA CTTTCCAGCG AGACAGCAAG 
CGCTGGTCTC GCATGGACCA GCTGGACCTT GATGTATTCT CCCCTACTGC AGGGGACACT 
GGCTCCTTCG CTGCATTCCT ATCCCAAGAT GAGCATTTTG TTAGAGGGCC CCTTCAAAAG 
GAGGGTGTTA ATGCCAGTGT GGAGAGCATC TTAACGGACC TTAGCGAGCA GCCAGAGGTG 
GGATCTCTGC ATAGTACTGG TAGTGGAGGT CGAGGAGGAT CACTGAGTAC CACTGTACCA 
TCCAGTCCTG CTACAACCAT CTCCAATGCT AATACAGCCA CCACAACAAG AGCAAGCTCA 
GTTGCCAGTG TCTGTTCTTC TGGAACATCA GGTGCCAATG AAGACTCCAT GTCTGATGGG 
CTTCCATCGC CCCTGGAACG TGGTACCTTT GCTTTTGATG TAAAACCAAA CTTGATAAGT 
AGTGCAGCTG AAAAAAGCAC ACGGTCAAGA GCAAAGAGCT TTTTAAAACG CATGGAAAGT 
TTACGTCTAC GCACCAGTAG CAATGCAGGC TCCAAGCGCA AGAAGAAAGG TGTTCCTGGG 
CATGGTAGGT TGGAGATCAG TGGGCCAGTG CTTAAGGAGG GTCTTGATGA AGACAAGCTG 
CGTCGGCTCA ACTGTGTTGA CATCGCAACT CTGGATCGAA ATGCAAACCG AAATCGAAGC 
ATCTCCTATT CAACGCAAAC AAGCAGTGGG AGTGCAGGGA GTAGCCAATC AGAAGCTAGC 
AGTGGGAGTG CTGTAAGTAC GCCCAGTCCA GTTTCAAGAG CACGTAGTCA CAGCACAGCA 
GCCGGGTCCA GCAAGAGAGG TGGGATGTAC TTGGAAGGTT TTGACCCATT TAACTTTGTT 
GTGCTGCAAC CTTCTGAAGA CCAAGATGTA AAGGAGAACC ATCAGACCCA AAATAAGAGG 
AAGGAGCCCA GCTCAACTGA TGAGAACAAT CGACGCAACC GTCAGAGTTC AGATAATAGG 
CAAATGGAGG ATGAAGAGAG TCGCGTAGAG GAAGGAGGTG TGATCTTTTT CTATTTGCCT 
GAAGGCCACA AGCCTGGCAC TTTTCCTAAA GCACTTGCGG ATGGAGGATC CTATCATGGT 
GTTGACAACA CAAGTCGCCG CCCACCCCGG AGGGGCTCTT CTGCTTCACT AGACAGTCGA 
ATGAGTATCT ATGATAATGT TCCTTTCTCG GAAGTGGGAG ATGATGAGGA CGAAGTAGGA 
GAGGAGGAAC CAAAACTGGA GGATGTGTTA GAGCGAGTCA GAGGTCTGCA GCAACTGGTC 
AATGAATGGC CAGAAACAGG AATTGGGGAA GAGGAGGAGG ATGAAGAGGA AGAGGAAGGA 
GATTCAGATT CAGCCCTTGA CTCTGCATCT CCTTGTCCAT CATCTCCACT GCAGAACCGC 
TTAGAAGAGA CAGAGAATGG CAGCGACCAG GACAGCACTG GAAACCCATT AGCAGAAAGA 
GATGAGACAC TCAGCTCACG TGAAAGATGT GACTCTGGAG TTGAAGCATC ACTTACTCGA 
GCCAACAGAA CTCAGAAACT GCGCTGGCCA AGTTTCCAGA GCTCCCACAG GCCAAGTCTG 
TCATCAACAG TGCTACAAGT TGGATGTCAG TCAGTTCTGC AGATGAATCT ACTCCAGAAA 
TACAGCCTTC TCAAACTTAC TGCTCTACTG GAGAGATACA CGCCCACCAA CAAACATGGC 
TTCAGCTGGG CTGTTCCTAA GTTTATGAAG CGGATCAAGG TTCGCGACTA CAAGGACAGG 
AGTGTGTTTG GCGTTCCACT GCAGGTGATA GTTCAAAGGA GCGGACAACC CCTGCCTCAG 
AGCATTCAAC AAGCCATGCG TTACCTGCGG AGCCAATGCC TCGACCAGGT GGGATTGTTC 
AGGAAATCAG GGGTGAAATC GCGAATCCAA GCCCTTCGCC AAATGAACGA ATCATGTGGC 
GCTGTTGGGG GAGGAGTCAA CTATGAGGGC CAATTAGCTT ATGACGTGGC TGACATGCTA 
AAGCAGTATT TCCGAGACCT TCCAGAGCCT CTGCTTACCA GCAAACTGTC TGATACCTTC 
CTGCAGATTT ATCAATATGT CCCCAAAGAG CAGCGCCTGC AGGCGGTAAG GGCTGCTGTG 
TTGCTGTTAC CAGACGAGAA CCGCGAGGCA CTGCAGACTC TGCTGTGCTT CCTGAGTGAT 
GTAACAGCCA GCGTGGGTGA AAACCAGATG ACCTGCACCA ACCTGGCCGT GTGTTTGGCC 
CCTTCTCTCT TCCACCTCAA CACACTGCGC AGAGAGAGCT CGTCACCCAG GGTCATGAAT 
AGAAAGAACA CCCTTGGAAA ACCTGACCAA AGAGACCTGA ATGAAAACCT GGCGGCTACT 
CATGGTTTAG CGCATATGAT ACAGGAGTGC AGAAAACTCT TCCAGATCCC AGAAGAGATG 
TCCCGATGTA GGAACTCTTA CATGGAGCAG GGCTTGAGTC CCATGAGGAT GGAGGTGTTG 
GCGGAAAACT GCGGTTCTTG CAACTACCAA AGTCTACTCA AAGACAGTAT TGATAGCCTG 
CTTAAAGAAG CCAAGGACAA ATTCAAAGGT TATGACAGCT GCTCGACTCC TGAAAACGCT 
GAGCTCGCAT ACAAGAAGGT TCAGGACGGC TCTTCTCTGC GGCAGTGGAA AGTTTGTGTG 
GATGTGCCTG CATCGGCTGA GGATGTCCTG CAACGCATTT TGCGAGAGCA GGAGCGCTGG 
GACGAGGACC TGCTAGAGTC CAGGATCGTG GAGACACTGG ACCACAACAC TGAAGTCTAT 
CAGTATGTCA GAAACTCAAT GCCACCCCAT CCGCCAAGAG ACCACGTGGT ACTCAGGTCA 
TGGATGACAG ATTTGCCCAA GGGAGTGTGT ACTTTAGTGA GCGTGTCAGT GGATCACGAG 
AGCGCAGCAG TGCTGGGTGT CCGTGCCAAT GTTCTGACAT CTCGCTATTA CATCGAGCCC 
TGCGGAGCCA ACAAGAGCCG CCTCGCACAC ATTTCCAGAA TAGACTGCAG GGGTCGTTGC 
CCTGAATGGT ACAATAAGCT ATATGGTCAC CTATGTGCTG CGGAGGTGGT GCGGATACGA 
GACACTTTTA CCTGTCTGGA CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159917.1
CDS	303..3689
Translation	MILTQIEAKEACTWLRAAGFPQYAQLYEDGQFPIEISSVTRDHDFLDQDAIEALCRRLNTLNKCALMRLEITPQRKRSEDSDEDEPCAISGRWTFQRDSKRWSRMDQLDLDVFSPTAGDTGSFAAFLSQDEHFVRGPLQKEGVNASVESILTDLSEQPEVGSLHSTGSGGRGGSLSTTVPSSPATTISNANTATTTRASSVASVCSSGTSGANEDSMSDGLPSPLERGTFAFDVKPNLISSAAEKSTRSRAKSFLKRMESLRLRTSSNAGSKRKKKGVPGHGRLEISGPVLKEGLDEDKLRRLNCVDIATLDRNANRNRSISYSTQTSSGSAGSSQSEASSGSAVSTPSPVSRARSHSTAAGSSKRGGMYLEGFDPFNFVVLQPSEDQDVKENHQTQNKRKEPSSTDENNRRNRQSSDNRQMEDEESRVEEGGVIFFYLPEGHKPGTFPKALADGGSYHGVDNTSRRPPRRGSSASLDSRMSIYDNVPFSEVGDDEDEVGEEEPKLEDVLERVRGLQQLVNEWPETGIGEEEEDEEEEEGDSDSALDSASPCPSSPLQNRLEETENGSDQDSTGNPLAERDETLSSRERCDSGVEASLTRANRTQKLRWPSFQSSHRPSLSSTVLQVGCQSVLQMNLLQKYSLLKLTALLERYTPTNKHGFSWAVPKFMKRIKVRDYKDRSVFGVPLQVIVQRSGQPLPQSIQQAMRYLRSQCLDQVGLFRKSGVKSRIQALRQMNESCGAVGGGVNYEGQLAYDVADMLKQYFRDLPEPLLTSKLSDTFLQIYQYVPKEQRLQAVRAAVLLLPDENREALQTLLCFLSDVTASVGENQMTCTNLAVCLAPSLFHLNTLRRESSSPRVMNRKNTLGKPDQRDLNENLAATHGLAHMIQECRKLFQIPEEMSRCRNSYMEQGLSPMRMEVLAENCGSCNYQSLLKDSIDSLLKEAKDKFKGYDSCSTPENAELAYKKVQDGSSLRQWKVCVDVPASAEDVLQRILREQERWDEDLLESRIVETLDHNTEVYQYVRNSMPPHPPRDHVVLRSWMTDLPKGVCTLVSVSVDHESAAVLGVRANVLTSRYYIEPCGANKSRLAHISRIDCRGRCPEWYNKLYGHLCAAEVVRIRDTFTCLDK

Target ORF information:

RefSeq Version	XM_005159860.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DLC1 Rho GTPase activating protein (dlc1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159860.3

ORF Insert Sequence:

ATGATCCTTA CTCAAATTGA GGCGAAGGAG GCGTGTACAT GGCTGAGGGC TGCAGGTTTT 
CCACAGTATG CTCAGCTTTA TGAAGATGGT CAGTTCCCGA TTGAGATTTC CTCAGTTACC 
AGAGATCACG ACTTCCTGGA TCAAGATGCC ATAGAAGCCT TGTGCAGGAG ACTGAACACC 
CTAAACAAGT GCGCTCTTAT GAGATTAGAG ATCACCCCTC AAAGAAAGAG AAGTGAAGAC 
TCTGATGAGG ATGAGCCATG TGCCATAAGT GGCCGTTGGA CTTTCCAGCG AGACAGCAAG 
CGCTGGTCTC GCATGGACCA GCTGGACCTT GATGTATTCT CCCCTACTGC AGGGGACACT 
GGCTCCTTCG CTGCATTCCT ATCCCAAGAT GAGCATTTTG TTAGAGGGCC CCTTCAAAAG 
GAGGGTGTTA ATGCCAGTGT GGAGAGCATC TTAACGGACC TTAGCGAGCA GCCAGAGGTG 
GGATCTCTGC ATAGTACTGG TAGTGGAGGT CGAGGAGGAT CACTGAGTAC CACTGTACCA 
TCCAGTCCTG CTACAACCAT CTCCAATGCT AATACAGCCA CCACAACAAG AGCAAGCTCA 
GTTGCCAGTG TCTGTTCTTC TGGAACATCA GGTGCCAATG AAGACTCCAT GTCTGATGGG 
CTTCCATCGC CCCTGGAACG TGGTACCTTT GCTTTTGATG TAAAACCAAA CTTGATAAGT 
AGTGCAGCTG AAAAAAGCAC ACGGTCAAGA GCAAAGAGCT TTTTAAAACG CATGGAAAGT 
TTACGTCTAC GCACCAGTAG CAATGCAGGC TCCAAGCGCA AGAAGAAAGG TGTTCCTGGG 
CATGGTAGGT TGGAGATCAG TGGGCCAGTG CTTAAGGAGG GTCTTGATGA AGACAAGCTG 
CGTCGGCTCA ACTGTGTTGA CATCGCAACT CTGGATCGAA ATGCAAACCG AAATCGAAGC 
ATCTCCTATT CAACGCAAAC AAGCAGTGGG AGTGCAGGGA GTAGCCAATC AGAAGCTAGC 
AGTGGGAGTG CTGTAAGTAC GCCCAGTCCA GTTTCAAGAG CACGTAGTCA CAGCACAGCA 
GCCGGGTCCA GCAAGAGAGG TGGGATGTAC TTGGAAGGTT TTGACCCATT TAACTTTGTT 
GTGCTGCAAC CTTCTGAAGA CCAAGATGTA AAGGAGAACC ATCAGACCCA AAATAAGAGG 
AAGGAGCCCA GCTCAACTGA TGAGAACAAT CGACGCAACC GTCAGAGTTC AGATAATAGG 
CAAATGGAGG ATGAAGAGAG TCGCGTAGAG GAAGGAGGTG TGATCTTTTT CTATTTGCCT 
GAAGGCCACA AGCCTGGCAC TTTTCCTAAA GCACTTGCGG ATGGAGGATC CTATCATGGT 
GTTGACAACA CAAGTCGCCG CCCACCCCGG AGGGGCTCTT CTGCTTCACT AGACAGTCGA 
ATGAGTATCT ATGATAATGT TCCTTTCTCG GAAGTGGGAG ATGATGAGGA CGAAGTAGGA 
GAGGAGGAAC CAAAACTGGA GGATGTGTTA GAGCGAGTCA GAGGTCTGCA GCAACTGGTC 
AATGAATGGC CAGAAACAGG AATTGGGGAA GAGGAGGAGG ATGAAGAGGA AGAGGAAGGA 
GATTCAGATT CAGCCCTTGA CTCTGCATCT CCTTGTCCAT CATCTCCACT GCAGAACCGC 
TTAGAAGAGA CAGAGAATGG CAGCGACCAG GACAGCACTG GAAACCCATT AGCAGAAAGA 
GATGAGACAC TCAGCTCACG TGAAAGATGT GACTCTGGAG TTGAAGCATC ACTTACTCGA 
GCCAACAGAA CTCAGAAACT GCGCTGGCCA AGTTTCCAGA GCTCCCACAG GCCAAGTCTG 
TCATCAACAG TGCTACAAGT TGGATGTCAG TCAGTTCTGC AGATGAATCT ACTCCAGAAA 
TACAGCCTTC TCAAACTTAC TGCTCTACTG GAGAGATACA CGCCCACCAA CAAACATGGC 
TTCAGCTGGG CTGTTCCTAA GTTTATGAAG CGGATCAAGG TTCGCGACTA CAAGGACAGG 
AGTGTGTTTG GCGTTCCACT GCAGGTGATA GTTCAAAGGA GCGGACAACC CCTGCCTCAG 
AGCATTCAAC AAGCCATGCG TTACCTGCGG AGCCAATGCC TCGACCAGGT GGGATTGTTC 
AGGAAATCAG GGGTGAAATC GCGAATCCAA GCCCTTCGCC AAATGAACGA ATCATGTGGC 
GCTGTTGGGG GAGGAGTCAA CTATGAGGGC CAATTAGCTT ATGACGTGGC TGACATGCTA 
AAGCAGTATT TCCGAGACCT TCCAGAGCCT CTGCTTACCA GCAAACTGTC TGATACCTTC 
CTGCAGATTT ATCAATATGT CCCCAAAGAG CAGCGCCTGC AGGCGGTAAG GGCTGCTGTG 
TTGCTGTTAC CAGACGAGAA CCGCGAGGCA CTGCAGACTC TGCTGTGCTT CCTGAGTGAT 
GTAACAGCCA GCGTGGGTGA AAACCAGATG ACCTGCACCA ACCTGGCCGT GTGTTTGGCC 
CCTTCTCTCT TCCACCTCAA CACACTGCGC AGAGAGAGCT CGTCACCCAG GGTCATGAAT 
AGAAAGAACA CCCTTGGAAA ACCTGACCAA AGAGACCTGA ATGAAAACCT GGCGGCTACT 
CATGGTTTAG CGCATATGAT ACAGGAGTGC AGAAAACTCT TCCAGATCCC AGAAGAGATG 
TCCCGATGTA GGAACTCTTA CATGGAGCAG GGCTTGAGTC CCATGAGGAT GGAGGTGTTG 
GCGGAAAACT GCGGTTCTTG CAACTACCAA AGTCTACTCA AAGACAGTAT TGATAGCCTG 
CTTAAAGAAG CCAAGGACAA ATTCAAAGGT TATGACAGCT GCTCGACTCC TGAAAACGCT 
GAGCTCGCAT ACAAGAAGGT TCAGGACGGC TCTTCTCTGC GGCAGTGGAA AGTTTGTGTG 
GATGTGCCTG CATCGGCTGA GGATGTCCTG CAACGCATTT TGCGAGAGCA GGAGCGCTGG 
GACGAGGACC TGCTAGAGTC CAGGATCGTG GAGACACTGG ACCACAACAC TGAAGTCTAT 
CAGTATGTCA GAAACTCAAT GCCACCCCAT CCGCCAAGAG ACCACGTGGT ACTCAGGTCA 
TGGATGACAG ATTTGCCCAA GGGAGTGTGT ACTTTAGTGA GCGTGTCAGT GGATCACGAG 
AGCGCAGCAG TGCTGGGTGT CCGTGCCAAT GTTCTGACAT CTCGCTATTA CATCGAGCCC 
TGCGGAGCCA ACAAGAGCCG CCTCGCACAC ATTTCCAGAA TAGACTGCAG GGGTCGTTGC 
CCTGAATGGT ACAATAAGCT ATATGGTCAC CTATGTGCTG CGGAGGTGGT GCGGATACGA 
GACACTTTTA CCTGTCTGGA CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dlc1 ( XM_001921739.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001921774.4 Danio rerio DLC1 Rho GTPase activating protein (dlc1), transcript variant X2, mRNA.

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CloneID	ODa53869
Clone ID Related Accession (Same CDS sequence)	XM_001921739.6 , XM_017355992.1 , XM_017355991.1 , XM_017355991.2
Accession Version	XM_001921739.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho GTPase-activating protein 7 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001921739.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTTG CCATTCGTAA TAGAAGCTGG GAGGATCACG TGACTTCACC ACGAACAGGA 
TTACAGTACA GCTATGATGA CTTAGACTTG GTTTGCTGCC ATGCAAGGCA CCGGCGAGGT 
AGATGCGCCT CCATGCCAGA ATGTGGCCAT CATCATCTTT ACCCTCAGGA TGATATTGGA 
AATGGTACAA AAAAGATGGA GAGGATATTG CAAAAGTTTA AGAATGAGAC TCAACCTAAC 
TGTGAAGATG TAAAGAGCCA ATTGTTGACA TCTAGAGATG CTGTCAATGA AAAGGCACAG 
CTTGAGAGTG AGCAAGATAA CAGACTACTT TCAAACAACG TGGCAGATCA AGATGAATTA 
CAGCATTCTG CTTTGGGAAA TTTGCAAACT CACAATGGAG ATGCAGATAA TAAGAAGCTA 
AACAAGGATA AAGTGTCTTC AGCCATTAGC TTGTCACATC TTGTGCCTTC TGCTGAAGCG 
TTGGATCAAA CAAATGGTTT AGGAAATGTT TTGAAGGATG GCATCTCCTT AAACAACACA 
GTCAAAGAAG ACTGCTCTGA GCTAACTCAT CCCAAAGAAG AAACATCTGT GGAAAACACA 
GAATCTATGG TGAAGGTCAA TGTGGGCAGT GAGAAGTTAG TATATGAACA CATGAATGTG 
ACAAGCAGCA TCACAGAAAT AACTAATAAA CCTCCCACTT CTGATTCATC TGCTGACCCG 
TGTGATAATG AGGAAGGACA TTGTTGTGAA ACATCTGAAC AGGCAATTTT AAAATGTATT 
AAAACTGAAC GCTCAAGCAA ACCTATGTCA TCATCATCAA CTTCTCAAGA AGATCATCAC 
ATTCCCCACA CAGAAGATGC TAATAAAGCA AATCTAGCAT GCAAAGATTG CAATATGACT 
CCAGAAGAAA ATGGAGATGG TATTTTTAAG TGTGCTGATG GTGATACGTT AAACGTTGGA 
TGCATTAACC CACATGCTTG TGAAAGTTCT CAACTGAAAG AATGTGGAGT AATAGACATC 
ATGGATACAT GGCATGACTG CTCGTCCAAA CTCCAAACCA TCCATGAGGG GTCGTGTCCT 
GAATCTGGAG ATGATTCTTC TGCTCAGATA TCAGGTGATG GGCCAGAGGA ACCAACATGC 
AGAGGATTAA ACACAAGGAA CTGTGAAAAT CTGACAGCCT CAGAGGATCT CAGAGAACCT 
CAACCTGAAG GAATCTATGA GGATCATCAG GCGACTCAAA ACACTGCAGC CAAGGACTGT 
GAGATGGTCC CCAAAGCTGA TGGTAGCATT GTCAAGCTTC GCACAAGAAA GAGCTCACAT 
GAAGAGAGGG AGAAGGAGAG GTCCCGGCTG GACTCAATGG TTCTACTAAT AATGAAGCTG 
GATCAGCTGG ACAAAGAGAT TGAAAATGCT CTAAGCTCCT CCCTAAATAG CACACCCAAT 
TTAAAGAGGC GGATCATTTC TGATGTTGAC TTAGAAACTC TGGATTCAGC CGGAAGTCTT 
TCAGCCTCCT CACAGTCTCT AAACACCCCA CTTTATCGCT CTTCTTCATC GTTGTCTTCA 
TCAGGACCCA CCGGAACCCC TGGAGCAAGA CCAAAAAATG AGACTTCTGC TGTACTTTCT 
GAGAACGACA GAGCAGAAAT TGAGGCGAAG GAGGCGTGTA CATGGCTGAG GGCTGCAGGT 
TTTCCACAGT ATGCTCAGCT TTATGAAGAT GGTCAGTTCC CGATTGAGAT TTCCTCAGTT 
ACCAGAGATC ACGACTTCCT GGATCAAGAT GCCATAGAAG CCTTGTGCAG GAGACTGAAC 
ACCCTAAACA AGTGCGCTCT TATGAGATTA GAGATCACCC CTCAAAGAAA GAGAAGTGAA 
GACTCTGATG AGGATGAGCC ATGTGCCATA AGTGGCCGTT GGACTTTCCA GCGAGACAGC 
AAGCGCTGGT CTCGCATGGA CCAGCTGGAC CTTGATGTAT TCTCCCCTAC TGCAGGGGAC 
ACTGGCTCCT TCGCTGCATT CCTATCCCAA GATGAGCATT TTGTTAGAGG GCCCCTTCAA 
AAGGAGGGTG TTAATGCCAG TGTGGAGAGC ATCTTAACGG ACCTTAGCGA GCAGCCAGAG 
GTGGGATCTC TGCATAGTAC TGGTAGTGGA GGTCGAGGAG GATCACTGAG TACCACTGTA 
CCATCCAGTC CTGCTACAAC CATCTCCAAT GCTAATACAG CCACCACAAC AAGAGCAAGC 
TCAGTTGCCA GTGTCTGTTC TTCTGGAACA TCAGGTGCCA ATGAAGACTC CATGTCTGAT 
GGGCTTCCAT CGCCCCTGGA ACGTGGTACC TTTGCTTTTG ATGTAAAACC AAACTTGATA 
AGTAGTGCAG CTGAAAAAAG CACACGGTCA AGAGCAAAGA GCTTTTTAAA ACGCATGGAA 
AGTTTACGTC TACGCACCAG TAGCAATGCA GGCTCCAAGC GCAAGAAGAA AGGTGTTCCT 
GGGCATGGTA GGTTGGAGAT CAGTGGGCCA GTGCTTAAGG AGGGTCTTGA TGAAGACAAG 
CTGCGTCGGC TCAACTGTGT TGACATCGCA ACTCTGGATC GAAATGCAAA CCGAAATCGA 
AGCATCTCCT ATTCAACGCA AACAAGCAGT GGGAGTGCAG GGAGTAGCCA ATCAGAAGCT 
AGCAGTGGGA GTGCTGTAAG TACGCCCAGT CCAGTTTCAA GAGCACGTAG TCACAGCACA 
GCAGCCGGGT CCAGCAAGAG AGGTGGGATG TACTTGGAAG GTTTTGACCC ATTTAACTTT 
GTTGTGCTGC AACCTTCTGA AGACCAAGAT GTAAAGGAGA ACCATCAGAC CCAAAATAAG 
AGGAAGGAGC CCAGCTCAAC TGATGAGAAC AATCGACGCA ACCGTCAGAG TTCAGATAAT 
AGGCAAATGG AGGATGAAGA GAGTCGCGTA GAGGAAGGAG GTGTGATCTT TTTCTATTTG 
CCTGAAGGCC ACAAGCCTGG CACTTTTCCT AAAGCACTTG CGGATGGAGG ATCCTATCAT 
GGTGTTGACA ACACAAGTCG CCGCCCACCC CGGAGGGGCT CTTCTGCTTC ACTAGACAGT 
CGAATGAGTA TCTATGATAA TGTTCCTTTC TCGGAAGTGG GAGATGATGA GGACGAAGTA 
GGAGAGGAGG AACCAAAACT GGAGGATGTG TTAGAGCGAG TCAGAGGTCT GCAGCAACTG 
GTCAATGAAT GGCCAGAAAC AGGAATTGGG GAAGAGGAGG AGGATGAAGA GGAAGAGGAA 
GGAGATTCAG ATTCAGCCCT TGACTCTGCA TCTCCTTGTC CATCATCTCC ACTGCAGAAC 
CGCTTAGAAG AGACAGAGAA TGGCAGCGAC CAGGACAGCA CTGGAAACCC ATTAGCAGAA 
AGAGATGAGA CACTCAGCTC ACGTGAAAGA TGTGACTCTG GAGTTGAAGC ATCACTTACT 
CGAGCCAACA GAACTCAGAA ACTGCGCTGG CCAAGTTTCC AGAGCTCCCA CAGGCCAAGT 
CTGTCATCAA CAGTGCTACA AGTTGGATGT CAGTCAGTTC TGCAGATGAA TCTACTCCAG 
AAATACAGCC TTCTCAAACT TACTGCTCTA CTGGAGAGAT ACACGCCCAC CAACAAACAT 
GGCTTCAGCT GGGCTGTTCC TAAGTTTATG AAGCGGATCA AGGTTCGCGA CTACAAGGAC 
AGGAGTGTGT TTGGCGTTCC ACTGCAGGTG ATAGTTCAAA GGAGCGGACA ACCCCTGCCT 
CAGAGCATTC AACAAGCCAT GCGTTACCTG CGGAGCCAAT GCCTCGACCA GGTGGGATTG 
TTCAGGAAAT CAGGGGTGAA ATCGCGAATC CAAGCCCTTC GCCAAATGAA CGAATCATGT 
GGCGCTGTTG GGGGAGGAGT CAACTATGAG GGCCAATTAG CTTATGACGT GGCTGACATG 
CTAAAGCAGT ATTTCCGAGA CCTTCCAGAG CCTCTGCTTA CCAGCAAACT GTCTGATACC 
TTCCTGCAGA TTTATCAATA TGTCCCCAAA GAGCAGCGCC TGCAGGCGGT AAGGGCTGCT 
GTGTTGCTGT TACCAGACGA GAACCGCGAG GCACTGCAGA CTCTGCTGTG CTTCCTGAGT 
GATGTAACAG CCAGCGTGGG TGAAAACCAG ATGACCTGCA CCAACCTGGC CGTGTGTTTG 
GCCCCTTCTC TCTTCCACCT CAACACACTG CGCAGAGAGA GCTCGTCACC CAGGGTCATG 
AATAGAAAGA ACACCCTTGG AAAACCTGAC CAAAGAGACC TGAATGAAAA CCTGGCGGCT 
ACTCATGGTT TAGCGCATAT GATACAGGAG TGCAGAAAAC TCTTCCAGAT CCCAGAAGAG 
ATGTCCCGAT GTAGGAACTC TTACATGGAG CAGGGCTTGA GTCCCATGAG GATGGAGGTG 
TTGGCGGAAA ACTGCGGTTC TTGCAACTAC CAAAGTCTAC TCAAAGACAG TATTGATAGC 
CTGCTTAAAG AAGCCAAGGA CAAATTCAAA GGTTATGACA GCTGCTCGAC TCCTGAAAAC 
GCTGAGCTCG CATACAAGAA GGTTCAGGAC GGCTCTTCTC TGCGGCAGTG GAAAGTTTGT 
GTGGATGTGC CTGCATCGGC TGAGGATGTC CTGCAACGCA TTTTGCGAGA GCAGGAGCGC 
TGGGACGAGG ACCTGCTAGA GTCCAGGATC GTGGAGACAC TGGACCACAA CACTGAAGTC 
TATCAGTATG TCAGAAACTC AATGCCACCC CATCCGCCAA GAGACCACGT GGTACTCAGG 
TCATGGATGA CAGATTTGCC CAAGGGAGTG TGTACTTTAG TGAGCGTGTC AGTGGATCAC 
GAGAGCGCAG CAGTGCTGGG TGTCCGTGCC AATGTTCTGA CATCTCGCTA TTACATCGAG 
CCCTGCGGAG CCAACAAGAG CCGCCTCGCA CACATTTCCA GAATAGACTG CAGGGGTCGT 
TGCCCTGAAT GGTACAATAA GCTATATGGT CACCTATGTG CTGCGGAGGT GGTGCGGATA 
CGAGACACTT TTACCTGTCT GGACAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001921774.4
CDS	843..5852
Translation	MPVAIRNRSWEDHVTSPRTGLQYSYDDLDLVCCHARHRRGRCASMPECGHHHLYPQDDIGNGTKKMERILQKFKNETQPNCEDVKSQLLTSRDAVNEKAQLESEQDNRLLSNNVADQDELQHSALGNLQTHNGDADNKKLNKDKVSSAISLSHLVPSAEALDQTNGLGNVLKDGISLNNTVKEDCSELTHPKEETSVENTESMVKVNVGSEKLVYEHMNVTSSITEITNKPPTSDSSADPCDNEEGHCCETSEQAILKCIKTERSSKPMSSSSTSQEDHHIPHTEDANKANLACKDCNMTPEENGDGIFKCADGDTLNVGCINPHACESSQLKECGVIDIMDTWHDCSSKLQTIHEGSCPESGDDSSAQISGDGPEEPTCRGLNTRNCENLTASEDLREPQPEGIYEDHQATQNTAAKDCEMVPKADGSIVKLRTRKSSHEEREKERSRLDSMVLLIMKLDQLDKEIENALSSSLNSTPNLKRRIISDVDLETLDSAGSLSASSQSLNTPLYRSSSSLSSSGPTGTPGARPKNETSAVLSENDRAEIEAKEACTWLRAAGFPQYAQLYEDGQFPIEISSVTRDHDFLDQDAIEALCRRLNTLNKCALMRLEITPQRKRSEDSDEDEPCAISGRWTFQRDSKRWSRMDQLDLDVFSPTAGDTGSFAAFLSQDEHFVRGPLQKEGVNASVESILTDLSEQPEVGSLHSTGSGGRGGSLSTTVPSSPATTISNANTATTTRASSVASVCSSGTSGANEDSMSDGLPSPLERGTFAFDVKPNLISSAAEKSTRSRAKSFLKRMESLRLRTSSNAGSKRKKKGVPGHGRLEISGPVLKEGLDEDKLRRLNCVDIATLDRNANRNRSISYSTQTSSGSAGSSQSEASSGSAVSTPSPVSRARSHSTAAGSSKRGGMYLEGFDPFNFVVLQPSEDQDVKENHQTQNKRKEPSSTDENNRRNRQSSDNRQMEDEESRVEEGGVIFFYLPEGHKPGTFPKALADGGSYHGVDNTSRRPPRRGSSASLDSRMSIYDNVPFSEVGDDEDEVGEEEPKLEDVLERVRGLQQLVNEWPETGIGEEEEDEEEEEGDSDSALDSASPCPSSPLQNRLEETENGSDQDSTGNPLAERDETLSSRERCDSGVEASLTRANRTQKLRWPSFQSSHRPSLSSTVLQVGCQSVLQMNLLQKYSLLKLTALLERYTPTNKHGFSWAVPKFMKRIKVRDYKDRSVFGVPLQVIVQRSGQPLPQSIQQAMRYLRSQCLDQVGLFRKSGVKSRIQALRQMNESCGAVGGGVNYEGQLAYDVADMLKQYFRDLPEPLLTSKLSDTFLQIYQYVPKEQRLQAVRAAVLLLPDENREALQTLLCFLSDVTASVGENQMTCTNLAVCLAPSLFHLNTLRRESSSPRVMNRKNTLGKPDQRDLNENLAATHGLAHMIQECRKLFQIPEEMSRCRNSYMEQGLSPMRMEVLAENCGSCNYQSLLKDSIDSLLKEAKDKFKGYDSCSTPENAELAYKKVQDGSSLRQWKVCVDVPASAEDVLQRILREQERWDEDLLESRIVETLDHNTEVYQYVRNSMPPHPPRDHVVLRSWMTDLPKGVCTLVSVSVDHESAAVLGVRANVLTSRYYIEPCGANKSRLAHISRIDCRGRCPEWYNKLYGHLCAAEVVRIRDTFTCLDK

Target ORF information:

RefSeq Version	XM_001921739.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DLC1 Rho GTPase activating protein (dlc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001921739.6

ORF Insert Sequence:

ATGCCTGTTG CCATTCGTAA TAGAAGCTGG GAGGATCACG TGACTTCACC ACGAACAGGA 
TTACAGTACA GCTATGATGA CTTAGACTTG GTTTGCTGCC ATGCAAGGCA CCGGCGAGGT 
AGATGCGCCT CCATGCCAGA ATGTGGCCAT CATCATCTTT ACCCTCAGGA TGATATTGGA 
AATGGTACAA AAAAGATGGA GAGGATATTG CAAAAGTTTA AGAATGAGAC TCAACCTAAC 
TGTGAAGATG TAAAGAGCCA ATTGTTGACA TCTAGAGATG CTGTCAATGA AAAGGCACAG 
CTTGAGAGTG AGCAAGATAA CAGACTACTT TCAAACAACG TGGCAGATCA AGATGAATTA 
CAGCATTCTG CTTTGGGAAA TTTGCAAACT CACAATGGAG ATGCAGATAA TAAGAAGCTA 
AACAAGGATA AAGTGTCTTC AGCCATTAGC TTGTCACATC TTGTGCCTTC TGCTGAAGCG 
TTGGATCAAA CAAATGGTTT AGGAAATGTT TTGAAGGATG GCATCTCCTT AAACAACACA 
GTCAAAGAAG ACTGCTCTGA GCTAACTCAT CCCAAAGAAG AAACATCTGT GGAAAACACA 
GAATCTATGG TGAAGGTCAA TGTGGGCAGT GAGAAGTTAG TATATGAACA CATGAATGTG 
ACAAGCAGCA TCACAGAAAT AACTAATAAA CCTCCCACTT CTGATTCATC TGCTGACCCG 
TGTGATAATG AGGAAGGACA TTGTTGTGAA ACATCTGAAC AGGCAATTTT AAAATGTATT 
AAAACTGAAC GCTCAAGCAA ACCTATGTCA TCATCATCAA CTTCTCAAGA AGATCATCAC 
ATTCCCCACA CAGAAGATGC TAATAAAGCA AATCTAGCAT GCAAAGATTG CAATATGACT 
CCAGAAGAAA ATGGAGATGG TATTTTTAAG TGTGCTGATG GTGATACGTT AAACGTTGGA 
TGCATTAACC CACATGCTTG TGAAAGTTCT CAACTGAAAG AATGTGGAGT AATAGACATC 
ATGGATACAT GGCATGACTG CTCGTCCAAA CTCCAAACCA TCCATGAGGG GTCGTGTCCT 
GAATCTGGAG ATGATTCTTC TGCTCAGATA TCAGGTGATG GGCCAGAGGA ACCAACATGC 
AGAGGATTAA ACACAAGGAA CTGTGAAAAT CTGACAGCCT CAGAGGATCT CAGAGAACCT 
CAACCTGAAG GAATCTATGA GGATCATCAG GCGACTCAAA ACACTGCAGC CAAGGACTGT 
GAGATGGTCC CCAAAGCTGA TGGTAGCATT GTCAAGCTTC GCACAAGAAA GAGCTCACAT 
GAAGAGAGGG AGAAGGAGAG GTCCCGGCTG GACTCAATGG TTCTACTAAT AATGAAGCTG 
GATCAGCTGG ACAAAGAGAT TGAAAATGCT CTAAGCTCCT CCCTAAATAG CACACCCAAT 
TTAAAGAGGC GGATCATTTC TGATGTTGAC TTAGAAACTC TGGATTCAGC CGGAAGTCTT 
TCAGCCTCCT CACAGTCTCT AAACACCCCA CTTTATCGCT CTTCTTCATC GTTGTCTTCA 
TCAGGACCCA CCGGAACCCC TGGAGCAAGA CCAAAAAATG AGACTTCTGC TGTACTTTCT 
GAGAACGACA GAGCAGAAAT TGAGGCGAAG GAGGCGTGTA CATGGCTGAG GGCTGCAGGT 
TTTCCACAGT ATGCTCAGCT TTATGAAGAT GGTCAGTTCC CGATTGAGAT TTCCTCAGTT 
ACCAGAGATC ACGACTTCCT GGATCAAGAT GCCATAGAAG CCTTGTGCAG GAGACTGAAC 
ACCCTAAACA AGTGCGCTCT TATGAGATTA GAGATCACCC CTCAAAGAAA GAGAAGTGAA 
GACTCTGATG AGGATGAGCC ATGTGCCATA AGTGGCCGTT GGACTTTCCA GCGAGACAGC 
AAGCGCTGGT CTCGCATGGA CCAGCTGGAC CTTGATGTAT TCTCCCCTAC TGCAGGGGAC 
ACTGGCTCCT TCGCTGCATT CCTATCCCAA GATGAGCATT TTGTTAGAGG GCCCCTTCAA 
AAGGAGGGTG TTAATGCCAG TGTGGAGAGC ATCTTAACGG ACCTTAGCGA GCAGCCAGAG 
GTGGGATCTC TGCATAGTAC TGGTAGTGGA GGTCGAGGAG GATCACTGAG TACCACTGTA 
CCATCCAGTC CTGCTACAAC CATCTCCAAT GCTAATACAG CCACCACAAC AAGAGCAAGC 
TCAGTTGCCA GTGTCTGTTC TTCTGGAACA TCAGGTGCCA ATGAAGACTC CATGTCTGAT 
GGGCTTCCAT CGCCCCTGGA ACGTGGTACC TTTGCTTTTG ATGTAAAACC AAACTTGATA 
AGTAGTGCAG CTGAAAAAAG CACACGGTCA AGAGCAAAGA GCTTTTTAAA ACGCATGGAA 
AGTTTACGTC TACGCACCAG TAGCAATGCA GGCTCCAAGC GCAAGAAGAA AGGTGTTCCT 
GGGCATGGTA GGTTGGAGAT CAGTGGGCCA GTGCTTAAGG AGGGTCTTGA TGAAGACAAG 
CTGCGTCGGC TCAACTGTGT TGACATCGCA ACTCTGGATC GAAATGCAAA CCGAAATCGA 
AGCATCTCCT ATTCAACGCA AACAAGCAGT GGGAGTGCAG GGAGTAGCCA ATCAGAAGCT 
AGCAGTGGGA GTGCTGTAAG TACGCCCAGT CCAGTTTCAA GAGCACGTAG TCACAGCACA 
GCAGCCGGGT CCAGCAAGAG AGGTGGGATG TACTTGGAAG GTTTTGACCC ATTTAACTTT 
GTTGTGCTGC AACCTTCTGA AGACCAAGAT GTAAAGGAGA ACCATCAGAC CCAAAATAAG 
AGGAAGGAGC CCAGCTCAAC TGATGAGAAC AATCGACGCA ACCGTCAGAG TTCAGATAAT 
AGGCAAATGG AGGATGAAGA GAGTCGCGTA GAGGAAGGAG GTGTGATCTT TTTCTATTTG 
CCTGAAGGCC ACAAGCCTGG CACTTTTCCT AAAGCACTTG CGGATGGAGG ATCCTATCAT 
GGTGTTGACA ACACAAGTCG CCGCCCACCC CGGAGGGGCT CTTCTGCTTC ACTAGACAGT 
CGAATGAGTA TCTATGATAA TGTTCCTTTC TCGGAAGTGG GAGATGATGA GGACGAAGTA 
GGAGAGGAGG AACCAAAACT GGAGGATGTG TTAGAGCGAG TCAGAGGTCT GCAGCAACTG 
GTCAATGAAT GGCCAGAAAC AGGAATTGGG GAAGAGGAGG AGGATGAAGA GGAAGAGGAA 
GGAGATTCAG ATTCAGCCCT TGACTCTGCA TCTCCTTGTC CATCATCTCC ACTGCAGAAC 
CGCTTAGAAG AGACAGAGAA TGGCAGCGAC CAGGACAGCA CTGGAAACCC ATTAGCAGAA 
AGAGATGAGA CACTCAGCTC ACGTGAAAGA TGTGACTCTG GAGTTGAAGC ATCACTTACT 
CGAGCCAACA GAACTCAGAA ACTGCGCTGG CCAAGTTTCC AGAGCTCCCA CAGGCCAAGT 
CTGTCATCAA CAGTGCTACA AGTTGGATGT CAGTCAGTTC TGCAGATGAA TCTACTCCAG 
AAATACAGCC TTCTCAAACT TACTGCTCTA CTGGAGAGAT ACACGCCCAC CAACAAACAT 
GGCTTCAGCT GGGCTGTTCC TAAGTTTATG AAGCGGATCA AGGTTCGCGA CTACAAGGAC 
AGGAGTGTGT TTGGCGTTCC ACTGCAGGTG ATAGTTCAAA GGAGCGGACA ACCCCTGCCT 
CAGAGCATTC AACAAGCCAT GCGTTACCTG CGGAGCCAAT GCCTCGACCA GGTGGGATTG 
TTCAGGAAAT CAGGGGTGAA ATCGCGAATC CAAGCCCTTC GCCAAATGAA CGAATCATGT 
GGCGCTGTTG GGGGAGGAGT CAACTATGAG GGCCAATTAG CTTATGACGT GGCTGACATG 
CTAAAGCAGT ATTTCCGAGA CCTTCCAGAG CCTCTGCTTA CCAGCAAACT GTCTGATACC 
TTCCTGCAGA TTTATCAATA TGTCCCCAAA GAGCAGCGCC TGCAGGCGGT AAGGGCTGCT 
GTGTTGCTGT TACCAGACGA GAACCGCGAG GCACTGCAGA CTCTGCTGTG CTTCCTGAGT 
GATGTAACAG CCAGCGTGGG TGAAAACCAG ATGACCTGCA CCAACCTGGC CGTGTGTTTG 
GCCCCTTCTC TCTTCCACCT CAACACACTG CGCAGAGAGA GCTCGTCACC CAGGGTCATG 
AATAGAAAGA ACACCCTTGG AAAACCTGAC CAAAGAGACC TGAATGAAAA CCTGGCGGCT 
ACTCATGGTT TAGCGCATAT GATACAGGAG TGCAGAAAAC TCTTCCAGAT CCCAGAAGAG 
ATGTCCCGAT GTAGGAACTC TTACATGGAG CAGGGCTTGA GTCCCATGAG GATGGAGGTG 
TTGGCGGAAA ACTGCGGTTC TTGCAACTAC CAAAGTCTAC TCAAAGACAG TATTGATAGC 
CTGCTTAAAG AAGCCAAGGA CAAATTCAAA GGTTATGACA GCTGCTCGAC TCCTGAAAAC 
GCTGAGCTCG CATACAAGAA GGTTCAGGAC GGCTCTTCTC TGCGGCAGTG GAAAGTTTGT 
GTGGATGTGC CTGCATCGGC TGAGGATGTC CTGCAACGCA TTTTGCGAGA GCAGGAGCGC 
TGGGACGAGG ACCTGCTAGA GTCCAGGATC GTGGAGACAC TGGACCACAA CACTGAAGTC 
TATCAGTATG TCAGAAACTC AATGCCACCC CATCCGCCAA GAGACCACGT GGTACTCAGG 
TCATGGATGA CAGATTTGCC CAAGGGAGTG TGTACTTTAG TGAGCGTGTC AGTGGATCAC 
GAGAGCGCAG CAGTGCTGGG TGTCCGTGCC AATGTTCTGA CATCTCGCTA TTACATCGAG 
CCCTGCGGAG CCAACAAGAG CCGCCTCGCA CACATTTCCA GAATAGACTG CAGGGGTCGT 
TGCCCTGAAT GGTACAATAA GCTATATGGT CACCTATGTG CTGCGGAGGT GGTGCGGATA 
CGAGACACTT TTACCTGTCT GGACAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dlc1 ( XM_017355992.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211481.1 Danio rerio DLC1 Rho GTPase activating protein (dlc1), transcript variant X3, mRNA.

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CloneID	ODa53869
Clone ID Related Accession (Same CDS sequence)	XM_001921739.6 , XM_017355992.1 , XM_017355991.1 , XM_017355991.2
Accession Version	XM_017355992.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho GTPase-activating protein 7 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTTG CCATTCGTAA TAGAAGCTGG GAGGATCACG TGACTTCACC ACGAACAGGA 
TTACAGTACA GCTATGATGA CTTAGACTTG GTTTGCTGCC ATGCAAGGCA CCGGCGAGGT 
AGATGCGCCT CCATGCCAGA ATGTGGCCAT CATCATCTTT ACCCTCAGGA TGATATTGGA 
AATGGTACAA AAAAGATGGA GAGGATATTG CAAAAGTTTA AGAATGAGAC TCAACCTAAC 
TGTGAAGATG TAAAGAGCCA ATTGTTGACA TCTAGAGATG CTGTCAATGA AAAGGCACAG 
CTTGAGAGTG AGCAAGATAA CAGACTACTT TCAAACAACG TGGCAGATCA AGATGAATTA 
CAGCATTCTG CTTTGGGAAA TTTGCAAACT CACAATGGAG ATGCAGATAA TAAGAAGCTA 
AACAAGGATA AAGTGTCTTC AGCCATTAGC TTGTCACATC TTGTGCCTTC TGCTGAAGCG 
TTGGATCAAA CAAATGGTTT AGGAAATGTT TTGAAGGATG GCATCTCCTT AAACAACACA 
GTCAAAGAAG ACTGCTCTGA GCTAACTCAT CCCAAAGAAG AAACATCTGT GGAAAACACA 
GAATCTATGG TGAAGGTCAA TGTGGGCAGT GAGAAGTTAG TATATGAACA CATGAATGTG 
ACAAGCAGCA TCACAGAAAT AACTAATAAA CCTCCCACTT CTGATTCATC TGCTGACCCG 
TGTGATAATG AGGAAGGACA TTGTTGTGAA ACATCTGAAC AGGCAATTTT AAAATGTATT 
AAAACTGAAC GCTCAAGCAA ACCTATGTCA TCATCATCAA CTTCTCAAGA AGATCATCAC 
ATTCCCCACA CAGAAGATGC TAATAAAGCA AATCTAGCAT GCAAAGATTG CAATATGACT 
CCAGAAGAAA ATGGAGATGG TATTTTTAAG TGTGCTGATG GTGATACGTT AAACGTTGGA 
TGCATTAACC CACATGCTTG TGAAAGTTCT CAACTGAAAG AATGTGGAGT AATAGACATC 
ATGGATACAT GGCATGACTG CTCGTCCAAA CTCCAAACCA TCCATGAGGG GTCGTGTCCT 
GAATCTGGAG ATGATTCTTC TGCTCAGATA TCAGGTGATG GGCCAGAGGA ACCAACATGC 
AGAGGATTAA ACACAAGGAA CTGTGAAAAT CTGACAGCCT CAGAGGATCT CAGAGAACCT 
CAACCTGAAG GAATCTATGA GGATCATCAG GCGACTCAAA ACACTGCAGC CAAGGACTGT 
GAGATGGTCC CCAAAGCTGA TGGTAGCATT GTCAAGCTTC GCACAAGAAA GAGCTCACAT 
GAAGAGAGGG AGAAGGAGAG GTCCCGGCTG GACTCAATGG TTCTACTAAT AATGAAGCTG 
GATCAGCTGG ACAAAGAGAT TGAAAATGCT CTAAGCTCCT CCCTAAATAG CACACCCAAT 
TTAAAGAGGC GGATCATTTC TGATGTTGAC TTAGAAACTC TGGATTCAGC CGGAAGTCTT 
TCAGCCTCCT CACAGTCTCT AAACACCCCA CTTTATCGCT CTTCTTCATC GTTGTCTTCA 
TCAGGACCCA CCGGAACCCC TGGAGCAAGA CCAAAAAATG AGACTTCTGC TGTACTTTCT 
GAGAACGACA GAGCAGAAAT TGAGGCGAAG GAGGCGTGTA CATGGCTGAG GGCTGCAGGT 
TTTCCACAGT ATGCTCAGCT TTATGAAGAT GGTCAGTTCC CGATTGAGAT TTCCTCAGTT 
ACCAGAGATC ACGACTTCCT GGATCAAGAT GCCATAGAAG CCTTGTGCAG GAGACTGAAC 
ACCCTAAACA AGTGCGCTCT TATGAGATTA GAGATCACCC CTCAAAGAAA GAGAAGTGAA 
GACTCTGATG AGGATGAGCC ATGTGCCATA AGTGGCCGTT GGACTTTCCA GCGAGACAGC 
AAGCGCTGGT CTCGCATGGA CCAGCTGGAC CTTGATGTAT TCTCCCCTAC TGCAGGGGAC 
ACTGGCTCCT TCGCTGCATT CCTATCCCAA GATGAGCATT TTGTTAGAGG GCCCCTTCAA 
AAGGAGGGTG TTAATGCCAG TGTGGAGAGC ATCTTAACGG ACCTTAGCGA GCAGCCAGAG 
GTGGGATCTC TGCATAGTAC TGGTAGTGGA GGTCGAGGAG GATCACTGAG TACCACTGTA 
CCATCCAGTC CTGCTACAAC CATCTCCAAT GCTAATACAG CCACCACAAC AAGAGCAAGC 
TCAGTTGCCA GTGTCTGTTC TTCTGGAACA TCAGGTGCCA ATGAAGACTC CATGTCTGAT 
GGGCTTCCAT CGCCCCTGGA ACGTGGTACC TTTGCTTTTG ATGTAAAACC AAACTTGATA 
AGTAGTGCAG CTGAAAAAAG CACACGGTCA AGAGCAAAGA GCTTTTTAAA ACGCATGGAA 
AGTTTACGTC TACGCACCAG TAGCAATGCA GGCTCCAAGC GCAAGAAGAA AGGTGTTCCT 
GGGCATGGTA GGTTGGAGAT CAGTGGGCCA GTGCTTAAGG AGGGTCTTGA TGAAGACAAG 
CTGCGTCGGC TCAACTGTGT TGACATCGCA ACTCTGGATC GAAATGCAAA CCGAAATCGA 
AGCATCTCCT ATTCAACGCA AACAAGCAGT GGGAGTGCAG GGAGTAGCCA ATCAGAAGCT 
AGCAGTGGGA GTGCTGTAAG TACGCCCAGT CCAGTTTCAA GAGCACGTAG TCACAGCACA 
GCAGCCGGGT CCAGCAAGAG AGGTGGGATG TACTTGGAAG GTTTTGACCC ATTTAACTTT 
GTTGTGCTGC AACCTTCTGA AGACCAAGAT GTAAAGGAGA ACCATCAGAC CCAAAATAAG 
AGGAAGGAGC CCAGCTCAAC TGATGAGAAC AATCGACGCA ACCGTCAGAG TTCAGATAAT 
AGGCAAATGG AGGATGAAGA GAGTCGCGTA GAGGAAGGAG GTGTGATCTT TTTCTATTTG 
CCTGAAGGCC ACAAGCCTGG CACTTTTCCT AAAGCACTTG CGGATGGAGG ATCCTATCAT 
GGTGTTGACA ACACAAGTCG CCGCCCACCC CGGAGGGGCT CTTCTGCTTC ACTAGACAGT 
CGAATGAGTA TCTATGATAA TGTTCCTTTC TCGGAAGTGG GAGATGATGA GGACGAAGTA 
GGAGAGGAGG AACCAAAACT GGAGGATGTG TTAGAGCGAG TCAGAGGTCT GCAGCAACTG 
GTCAATGAAT GGCCAGAAAC AGGAATTGGG GAAGAGGAGG AGGATGAAGA GGAAGAGGAA 
GGAGATTCAG ATTCAGCCCT TGACTCTGCA TCTCCTTGTC CATCATCTCC ACTGCAGAAC 
CGCTTAGAAG AGACAGAGAA TGGCAGCGAC CAGGACAGCA CTGGAAACCC ATTAGCAGAA 
AGAGATGAGA CACTCAGCTC ACGTGAAAGA TGTGACTCTG GAGTTGAAGC ATCACTTACT 
CGAGCCAACA GAACTCAGAA ACTGCGCTGG CCAAGTTTCC AGAGCTCCCA CAGGCCAAGT 
CTGTCATCAA CAGTGCTACA AGTTGGATGT CAGTCAGTTC TGCAGATGAA TCTACTCCAG 
AAATACAGCC TTCTCAAACT TACTGCTCTA CTGGAGAGAT ACACGCCCAC CAACAAACAT 
GGCTTCAGCT GGGCTGTTCC TAAGTTTATG AAGCGGATCA AGGTTCGCGA CTACAAGGAC 
AGGAGTGTGT TTGGCGTTCC ACTGCAGGTG ATAGTTCAAA GGAGCGGACA ACCCCTGCCT 
CAGAGCATTC AACAAGCCAT GCGTTACCTG CGGAGCCAAT GCCTCGACCA GGTGGGATTG 
TTCAGGAAAT CAGGGGTGAA ATCGCGAATC CAAGCCCTTC GCCAAATGAA CGAATCATGT 
GGCGCTGTTG GGGGAGGAGT CAACTATGAG GGCCAATTAG CTTATGACGT GGCTGACATG 
CTAAAGCAGT ATTTCCGAGA CCTTCCAGAG CCTCTGCTTA CCAGCAAACT GTCTGATACC 
TTCCTGCAGA TTTATCAATA TGTCCCCAAA GAGCAGCGCC TGCAGGCGGT AAGGGCTGCT 
GTGTTGCTGT TACCAGACGA GAACCGCGAG GCACTGCAGA CTCTGCTGTG CTTCCTGAGT 
GATGTAACAG CCAGCGTGGG TGAAAACCAG ATGACCTGCA CCAACCTGGC CGTGTGTTTG 
GCCCCTTCTC TCTTCCACCT CAACACACTG CGCAGAGAGA GCTCGTCACC CAGGGTCATG 
AATAGAAAGA ACACCCTTGG AAAACCTGAC CAAAGAGACC TGAATGAAAA CCTGGCGGCT 
ACTCATGGTT TAGCGCATAT GATACAGGAG TGCAGAAAAC TCTTCCAGAT CCCAGAAGAG 
ATGTCCCGAT GTAGGAACTC TTACATGGAG CAGGGCTTGA GTCCCATGAG GATGGAGGTG 
TTGGCGGAAA ACTGCGGTTC TTGCAACTAC CAAAGTCTAC TCAAAGACAG TATTGATAGC 
CTGCTTAAAG AAGCCAAGGA CAAATTCAAA GGTTATGACA GCTGCTCGAC TCCTGAAAAC 
GCTGAGCTCG CATACAAGAA GGTTCAGGAC GGCTCTTCTC TGCGGCAGTG GAAAGTTTGT 
GTGGATGTGC CTGCATCGGC TGAGGATGTC CTGCAACGCA TTTTGCGAGA GCAGGAGCGC 
TGGGACGAGG ACCTGCTAGA GTCCAGGATC GTGGAGACAC TGGACCACAA CACTGAAGTC 
TATCAGTATG TCAGAAACTC AATGCCACCC CATCCGCCAA GAGACCACGT GGTACTCAGG 
TCATGGATGA CAGATTTGCC CAAGGGAGTG TGTACTTTAG TGAGCGTGTC AGTGGATCAC 
GAGAGCGCAG CAGTGCTGGG TGTCCGTGCC AATGTTCTGA CATCTCGCTA TTACATCGAG 
CCCTGCGGAG CCAACAAGAG CCGCCTCGCA CACATTTCCA GAATAGACTG CAGGGGTCGT 
TGCCCTGAAT GGTACAATAA GCTATATGGT CACCTATGTG CTGCGGAGGT GGTGCGGATA 
CGAGACACTT TTACCTGTCT GGACAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211481.1
CDS	778..5787
Translation	MPVAIRNRSWEDHVTSPRTGLQYSYDDLDLVCCHARHRRGRCASMPECGHHHLYPQDDIGNGTKKMERILQKFKNETQPNCEDVKSQLLTSRDAVNEKAQLESEQDNRLLSNNVADQDELQHSALGNLQTHNGDADNKKLNKDKVSSAISLSHLVPSAEALDQTNGLGNVLKDGISLNNTVKEDCSELTHPKEETSVENTESMVKVNVGSEKLVYEHMNVTSSITEITNKPPTSDSSADPCDNEEGHCCETSEQAILKCIKTERSSKPMSSSSTSQEDHHIPHTEDANKANLACKDCNMTPEENGDGIFKCADGDTLNVGCINPHACESSQLKECGVIDIMDTWHDCSSKLQTIHEGSCPESGDDSSAQISGDGPEEPTCRGLNTRNCENLTASEDLREPQPEGIYEDHQATQNTAAKDCEMVPKADGSIVKLRTRKSSHEEREKERSRLDSMVLLIMKLDQLDKEIENALSSSLNSTPNLKRRIISDVDLETLDSAGSLSASSQSLNTPLYRSSSSLSSSGPTGTPGARPKNETSAVLSENDRAEIEAKEACTWLRAAGFPQYAQLYEDGQFPIEISSVTRDHDFLDQDAIEALCRRLNTLNKCALMRLEITPQRKRSEDSDEDEPCAISGRWTFQRDSKRWSRMDQLDLDVFSPTAGDTGSFAAFLSQDEHFVRGPLQKEGVNASVESILTDLSEQPEVGSLHSTGSGGRGGSLSTTVPSSPATTISNANTATTTRASSVASVCSSGTSGANEDSMSDGLPSPLERGTFAFDVKPNLISSAAEKSTRSRAKSFLKRMESLRLRTSSNAGSKRKKKGVPGHGRLEISGPVLKEGLDEDKLRRLNCVDIATLDRNANRNRSISYSTQTSSGSAGSSQSEASSGSAVSTPSPVSRARSHSTAAGSSKRGGMYLEGFDPFNFVVLQPSEDQDVKENHQTQNKRKEPSSTDENNRRNRQSSDNRQMEDEESRVEEGGVIFFYLPEGHKPGTFPKALADGGSYHGVDNTSRRPPRRGSSASLDSRMSIYDNVPFSEVGDDEDEVGEEEPKLEDVLERVRGLQQLVNEWPETGIGEEEEDEEEEEGDSDSALDSASPCPSSPLQNRLEETENGSDQDSTGNPLAERDETLSSRERCDSGVEASLTRANRTQKLRWPSFQSSHRPSLSSTVLQVGCQSVLQMNLLQKYSLLKLTALLERYTPTNKHGFSWAVPKFMKRIKVRDYKDRSVFGVPLQVIVQRSGQPLPQSIQQAMRYLRSQCLDQVGLFRKSGVKSRIQALRQMNESCGAVGGGVNYEGQLAYDVADMLKQYFRDLPEPLLTSKLSDTFLQIYQYVPKEQRLQAVRAAVLLLPDENREALQTLLCFLSDVTASVGENQMTCTNLAVCLAPSLFHLNTLRRESSSPRVMNRKNTLGKPDQRDLNENLAATHGLAHMIQECRKLFQIPEEMSRCRNSYMEQGLSPMRMEVLAENCGSCNYQSLLKDSIDSLLKEAKDKFKGYDSCSTPENAELAYKKVQDGSSLRQWKVCVDVPASAEDVLQRILREQERWDEDLLESRIVETLDHNTEVYQYVRNSMPPHPPRDHVVLRSWMTDLPKGVCTLVSVSVDHESAAVLGVRANVLTSRYYIEPCGANKSRLAHISRIDCRGRCPEWYNKLYGHLCAAEVVRIRDTFTCLDK

Target ORF information:

RefSeq Version	XM_017355992.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DLC1 Rho GTPase activating protein (dlc1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355992.1

ORF Insert Sequence:

ATGCCTGTTG CCATTCGTAA TAGAAGCTGG GAGGATCACG TGACTTCACC ACGAACAGGA 
TTACAGTACA GCTATGATGA CTTAGACTTG GTTTGCTGCC ATGCAAGGCA CCGGCGAGGT 
AGATGCGCCT CCATGCCAGA ATGTGGCCAT CATCATCTTT ACCCTCAGGA TGATATTGGA 
AATGGTACAA AAAAGATGGA GAGGATATTG CAAAAGTTTA AGAATGAGAC TCAACCTAAC 
TGTGAAGATG TAAAGAGCCA ATTGTTGACA TCTAGAGATG CTGTCAATGA AAAGGCACAG 
CTTGAGAGTG AGCAAGATAA CAGACTACTT TCAAACAACG TGGCAGATCA AGATGAATTA 
CAGCATTCTG CTTTGGGAAA TTTGCAAACT CACAATGGAG ATGCAGATAA TAAGAAGCTA 
AACAAGGATA AAGTGTCTTC AGCCATTAGC TTGTCACATC TTGTGCCTTC TGCTGAAGCG 
TTGGATCAAA CAAATGGTTT AGGAAATGTT TTGAAGGATG GCATCTCCTT AAACAACACA 
GTCAAAGAAG ACTGCTCTGA GCTAACTCAT CCCAAAGAAG AAACATCTGT GGAAAACACA 
GAATCTATGG TGAAGGTCAA TGTGGGCAGT GAGAAGTTAG TATATGAACA CATGAATGTG 
ACAAGCAGCA TCACAGAAAT AACTAATAAA CCTCCCACTT CTGATTCATC TGCTGACCCG 
TGTGATAATG AGGAAGGACA TTGTTGTGAA ACATCTGAAC AGGCAATTTT AAAATGTATT 
AAAACTGAAC GCTCAAGCAA ACCTATGTCA TCATCATCAA CTTCTCAAGA AGATCATCAC 
ATTCCCCACA CAGAAGATGC TAATAAAGCA AATCTAGCAT GCAAAGATTG CAATATGACT 
CCAGAAGAAA ATGGAGATGG TATTTTTAAG TGTGCTGATG GTGATACGTT AAACGTTGGA 
TGCATTAACC CACATGCTTG TGAAAGTTCT CAACTGAAAG AATGTGGAGT AATAGACATC 
ATGGATACAT GGCATGACTG CTCGTCCAAA CTCCAAACCA TCCATGAGGG GTCGTGTCCT 
GAATCTGGAG ATGATTCTTC TGCTCAGATA TCAGGTGATG GGCCAGAGGA ACCAACATGC 
AGAGGATTAA ACACAAGGAA CTGTGAAAAT CTGACAGCCT CAGAGGATCT CAGAGAACCT 
CAACCTGAAG GAATCTATGA GGATCATCAG GCGACTCAAA ACACTGCAGC CAAGGACTGT 
GAGATGGTCC CCAAAGCTGA TGGTAGCATT GTCAAGCTTC GCACAAGAAA GAGCTCACAT 
GAAGAGAGGG AGAAGGAGAG GTCCCGGCTG GACTCAATGG TTCTACTAAT AATGAAGCTG 
GATCAGCTGG ACAAAGAGAT TGAAAATGCT CTAAGCTCCT CCCTAAATAG CACACCCAAT 
TTAAAGAGGC GGATCATTTC TGATGTTGAC TTAGAAACTC TGGATTCAGC CGGAAGTCTT 
TCAGCCTCCT CACAGTCTCT AAACACCCCA CTTTATCGCT CTTCTTCATC GTTGTCTTCA 
TCAGGACCCA CCGGAACCCC TGGAGCAAGA CCAAAAAATG AGACTTCTGC TGTACTTTCT 
GAGAACGACA GAGCAGAAAT TGAGGCGAAG GAGGCGTGTA CATGGCTGAG GGCTGCAGGT 
TTTCCACAGT ATGCTCAGCT TTATGAAGAT GGTCAGTTCC CGATTGAGAT TTCCTCAGTT 
ACCAGAGATC ACGACTTCCT GGATCAAGAT GCCATAGAAG CCTTGTGCAG GAGACTGAAC 
ACCCTAAACA AGTGCGCTCT TATGAGATTA GAGATCACCC CTCAAAGAAA GAGAAGTGAA 
GACTCTGATG AGGATGAGCC ATGTGCCATA AGTGGCCGTT GGACTTTCCA GCGAGACAGC 
AAGCGCTGGT CTCGCATGGA CCAGCTGGAC CTTGATGTAT TCTCCCCTAC TGCAGGGGAC 
ACTGGCTCCT TCGCTGCATT CCTATCCCAA GATGAGCATT TTGTTAGAGG GCCCCTTCAA 
AAGGAGGGTG TTAATGCCAG TGTGGAGAGC ATCTTAACGG ACCTTAGCGA GCAGCCAGAG 
GTGGGATCTC TGCATAGTAC TGGTAGTGGA GGTCGAGGAG GATCACTGAG TACCACTGTA 
CCATCCAGTC CTGCTACAAC CATCTCCAAT GCTAATACAG CCACCACAAC AAGAGCAAGC 
TCAGTTGCCA GTGTCTGTTC TTCTGGAACA TCAGGTGCCA ATGAAGACTC CATGTCTGAT 
GGGCTTCCAT CGCCCCTGGA ACGTGGTACC TTTGCTTTTG ATGTAAAACC AAACTTGATA 
AGTAGTGCAG CTGAAAAAAG CACACGGTCA AGAGCAAAGA GCTTTTTAAA ACGCATGGAA 
AGTTTACGTC TACGCACCAG TAGCAATGCA GGCTCCAAGC GCAAGAAGAA AGGTGTTCCT 
GGGCATGGTA GGTTGGAGAT CAGTGGGCCA GTGCTTAAGG AGGGTCTTGA TGAAGACAAG 
CTGCGTCGGC TCAACTGTGT TGACATCGCA ACTCTGGATC GAAATGCAAA CCGAAATCGA 
AGCATCTCCT ATTCAACGCA AACAAGCAGT GGGAGTGCAG GGAGTAGCCA ATCAGAAGCT 
AGCAGTGGGA GTGCTGTAAG TACGCCCAGT CCAGTTTCAA GAGCACGTAG TCACAGCACA 
GCAGCCGGGT CCAGCAAGAG AGGTGGGATG TACTTGGAAG GTTTTGACCC ATTTAACTTT 
GTTGTGCTGC AACCTTCTGA AGACCAAGAT GTAAAGGAGA ACCATCAGAC CCAAAATAAG 
AGGAAGGAGC CCAGCTCAAC TGATGAGAAC AATCGACGCA ACCGTCAGAG TTCAGATAAT 
AGGCAAATGG AGGATGAAGA GAGTCGCGTA GAGGAAGGAG GTGTGATCTT TTTCTATTTG 
CCTGAAGGCC ACAAGCCTGG CACTTTTCCT AAAGCACTTG CGGATGGAGG ATCCTATCAT 
GGTGTTGACA ACACAAGTCG CCGCCCACCC CGGAGGGGCT CTTCTGCTTC ACTAGACAGT 
CGAATGAGTA TCTATGATAA TGTTCCTTTC TCGGAAGTGG GAGATGATGA GGACGAAGTA 
GGAGAGGAGG AACCAAAACT GGAGGATGTG TTAGAGCGAG TCAGAGGTCT GCAGCAACTG 
GTCAATGAAT GGCCAGAAAC AGGAATTGGG GAAGAGGAGG AGGATGAAGA GGAAGAGGAA 
GGAGATTCAG ATTCAGCCCT TGACTCTGCA TCTCCTTGTC CATCATCTCC ACTGCAGAAC 
CGCTTAGAAG AGACAGAGAA TGGCAGCGAC CAGGACAGCA CTGGAAACCC ATTAGCAGAA 
AGAGATGAGA CACTCAGCTC ACGTGAAAGA TGTGACTCTG GAGTTGAAGC ATCACTTACT 
CGAGCCAACA GAACTCAGAA ACTGCGCTGG CCAAGTTTCC AGAGCTCCCA CAGGCCAAGT 
CTGTCATCAA CAGTGCTACA AGTTGGATGT CAGTCAGTTC TGCAGATGAA TCTACTCCAG 
AAATACAGCC TTCTCAAACT TACTGCTCTA CTGGAGAGAT ACACGCCCAC CAACAAACAT 
GGCTTCAGCT GGGCTGTTCC TAAGTTTATG AAGCGGATCA AGGTTCGCGA CTACAAGGAC 
AGGAGTGTGT TTGGCGTTCC ACTGCAGGTG ATAGTTCAAA GGAGCGGACA ACCCCTGCCT 
CAGAGCATTC AACAAGCCAT GCGTTACCTG CGGAGCCAAT GCCTCGACCA GGTGGGATTG 
TTCAGGAAAT CAGGGGTGAA ATCGCGAATC CAAGCCCTTC GCCAAATGAA CGAATCATGT 
GGCGCTGTTG GGGGAGGAGT CAACTATGAG GGCCAATTAG CTTATGACGT GGCTGACATG 
CTAAAGCAGT ATTTCCGAGA CCTTCCAGAG CCTCTGCTTA CCAGCAAACT GTCTGATACC 
TTCCTGCAGA TTTATCAATA TGTCCCCAAA GAGCAGCGCC TGCAGGCGGT AAGGGCTGCT 
GTGTTGCTGT TACCAGACGA GAACCGCGAG GCACTGCAGA CTCTGCTGTG CTTCCTGAGT 
GATGTAACAG CCAGCGTGGG TGAAAACCAG ATGACCTGCA CCAACCTGGC CGTGTGTTTG 
GCCCCTTCTC TCTTCCACCT CAACACACTG CGCAGAGAGA GCTCGTCACC CAGGGTCATG 
AATAGAAAGA ACACCCTTGG AAAACCTGAC CAAAGAGACC TGAATGAAAA CCTGGCGGCT 
ACTCATGGTT TAGCGCATAT GATACAGGAG TGCAGAAAAC TCTTCCAGAT CCCAGAAGAG 
ATGTCCCGAT GTAGGAACTC TTACATGGAG CAGGGCTTGA GTCCCATGAG GATGGAGGTG 
TTGGCGGAAA ACTGCGGTTC TTGCAACTAC CAAAGTCTAC TCAAAGACAG TATTGATAGC 
CTGCTTAAAG AAGCCAAGGA CAAATTCAAA GGTTATGACA GCTGCTCGAC TCCTGAAAAC 
GCTGAGCTCG CATACAAGAA GGTTCAGGAC GGCTCTTCTC TGCGGCAGTG GAAAGTTTGT 
GTGGATGTGC CTGCATCGGC TGAGGATGTC CTGCAACGCA TTTTGCGAGA GCAGGAGCGC 
TGGGACGAGG ACCTGCTAGA GTCCAGGATC GTGGAGACAC TGGACCACAA CACTGAAGTC 
TATCAGTATG TCAGAAACTC AATGCCACCC CATCCGCCAA GAGACCACGT GGTACTCAGG 
TCATGGATGA CAGATTTGCC CAAGGGAGTG TGTACTTTAG TGAGCGTGTC AGTGGATCAC 
GAGAGCGCAG CAGTGCTGGG TGTCCGTGCC AATGTTCTGA CATCTCGCTA TTACATCGAG 
CCCTGCGGAG CCAACAAGAG CCGCCTCGCA CACATTTCCA GAATAGACTG CAGGGGTCGT 
TGCCCTGAAT GGTACAATAA GCTATATGGT CACCTATGTG CTGCGGAGGT GGTGCGGATA 
CGAGACACTT TTACCTGTCT GGACAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dlc1 ( XM_017355991.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211480.1 Danio rerio DLC1 Rho GTPase activating protein (dlc1), transcript variant X1, mRNA.

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CloneID	ODa53869
Clone ID Related Accession (Same CDS sequence)	XM_001921739.6 , XM_017355992.1 , XM_017355991.1 , XM_017355991.2
Accession Version	XM_017355991.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho GTPase-activating protein 7 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTTG CCATTCGTAA TAGAAGCTGG GAGGATCACG TGACTTCACC ACGAACAGGA 
TTACAGTACA GCTATGATGA CTTAGACTTG GTTTGCTGCC ATGCAAGGCA CCGGCGAGGT 
AGATGCGCCT CCATGCCAGA ATGTGGCCAT CATCATCTTT ACCCTCAGGA TGATATTGGA 
AATGGTACAA AAAAGATGGA GAGGATATTG CAAAAGTTTA AGAATGAGAC TCAACCTAAC 
TGTGAAGATG TAAAGAGCCA ATTGTTGACA TCTAGAGATG CTGTCAATGA AAAGGCACAG 
CTTGAGAGTG AGCAAGATAA CAGACTACTT TCAAACAACG TGGCAGATCA AGATGAATTA 
CAGCATTCTG CTTTGGGAAA TTTGCAAACT CACAATGGAG ATGCAGATAA TAAGAAGCTA 
AACAAGGATA AAGTGTCTTC AGCCATTAGC TTGTCACATC TTGTGCCTTC TGCTGAAGCG 
TTGGATCAAA CAAATGGTTT AGGAAATGTT TTGAAGGATG GCATCTCCTT AAACAACACA 
GTCAAAGAAG ACTGCTCTGA GCTAACTCAT CCCAAAGAAG AAACATCTGT GGAAAACACA 
GAATCTATGG TGAAGGTCAA TGTGGGCAGT GAGAAGTTAG TATATGAACA CATGAATGTG 
ACAAGCAGCA TCACAGAAAT AACTAATAAA CCTCCCACTT CTGATTCATC TGCTGACCCG 
TGTGATAATG AGGAAGGACA TTGTTGTGAA ACATCTGAAC AGGCAATTTT AAAATGTATT 
AAAACTGAAC GCTCAAGCAA ACCTATGTCA TCATCATCAA CTTCTCAAGA AGATCATCAC 
ATTCCCCACA CAGAAGATGC TAATAAAGCA AATCTAGCAT GCAAAGATTG CAATATGACT 
CCAGAAGAAA ATGGAGATGG TATTTTTAAG TGTGCTGATG GTGATACGTT AAACGTTGGA 
TGCATTAACC CACATGCTTG TGAAAGTTCT CAACTGAAAG AATGTGGAGT AATAGACATC 
ATGGATACAT GGCATGACTG CTCGTCCAAA CTCCAAACCA TCCATGAGGG GTCGTGTCCT 
GAATCTGGAG ATGATTCTTC TGCTCAGATA TCAGGTGATG GGCCAGAGGA ACCAACATGC 
AGAGGATTAA ACACAAGGAA CTGTGAAAAT CTGACAGCCT CAGAGGATCT CAGAGAACCT 
CAACCTGAAG GAATCTATGA GGATCATCAG GCGACTCAAA ACACTGCAGC CAAGGACTGT 
GAGATGGTCC CCAAAGCTGA TGGTAGCATT GTCAAGCTTC GCACAAGAAA GAGCTCACAT 
GAAGAGAGGG AGAAGGAGAG GTCCCGGCTG GACTCAATGG TTCTACTAAT AATGAAGCTG 
GATCAGCTGG ACAAAGAGAT TGAAAATGCT CTAAGCTCCT CCCTAAATAG CACACCCAAT 
TTAAAGAGGC GGATCATTTC TGATGTTGAC TTAGAAACTC TGGATTCAGC CGGAAGTCTT 
TCAGCCTCCT CACAGTCTCT AAACACCCCA CTTTATCGCT CTTCTTCATC GTTGTCTTCA 
TCAGGACCCA CCGGAACCCC TGGAGCAAGA CCAAAAAATG AGACTTCTGC TGTACTTTCT 
GAGAACGACA GAGCAGAAAT TGAGGCGAAG GAGGCGTGTA CATGGCTGAG GGCTGCAGGT 
TTTCCACAGT ATGCTCAGCT TTATGAAGAT GGTCAGTTCC CGATTGAGAT TTCCTCAGTT 
ACCAGAGATC ACGACTTCCT GGATCAAGAT GCCATAGAAG CCTTGTGCAG GAGACTGAAC 
ACCCTAAACA AGTGCGCTCT TATGAGATTA GAGATCACCC CTCAAAGAAA GAGAAGTGAA 
GACTCTGATG AGGATGAGCC ATGTGCCATA AGTGGCCGTT GGACTTTCCA GCGAGACAGC 
AAGCGCTGGT CTCGCATGGA CCAGCTGGAC CTTGATGTAT TCTCCCCTAC TGCAGGGGAC 
ACTGGCTCCT TCGCTGCATT CCTATCCCAA GATGAGCATT TTGTTAGAGG GCCCCTTCAA 
AAGGAGGGTG TTAATGCCAG TGTGGAGAGC ATCTTAACGG ACCTTAGCGA GCAGCCAGAG 
GTGGGATCTC TGCATAGTAC TGGTAGTGGA GGTCGAGGAG GATCACTGAG TACCACTGTA 
CCATCCAGTC CTGCTACAAC CATCTCCAAT GCTAATACAG CCACCACAAC AAGAGCAAGC 
TCAGTTGCCA GTGTCTGTTC TTCTGGAACA TCAGGTGCCA ATGAAGACTC CATGTCTGAT 
GGGCTTCCAT CGCCCCTGGA ACGTGGTACC TTTGCTTTTG ATGTAAAACC AAACTTGATA 
AGTAGTGCAG CTGAAAAAAG CACACGGTCA AGAGCAAAGA GCTTTTTAAA ACGCATGGAA 
AGTTTACGTC TACGCACCAG TAGCAATGCA GGCTCCAAGC GCAAGAAGAA AGGTGTTCCT 
GGGCATGGTA GGTTGGAGAT CAGTGGGCCA GTGCTTAAGG AGGGTCTTGA TGAAGACAAG 
CTGCGTCGGC TCAACTGTGT TGACATCGCA ACTCTGGATC GAAATGCAAA CCGAAATCGA 
AGCATCTCCT ATTCAACGCA AACAAGCAGT GGGAGTGCAG GGAGTAGCCA ATCAGAAGCT 
AGCAGTGGGA GTGCTGTAAG TACGCCCAGT CCAGTTTCAA GAGCACGTAG TCACAGCACA 
GCAGCCGGGT CCAGCAAGAG AGGTGGGATG TACTTGGAAG GTTTTGACCC ATTTAACTTT 
GTTGTGCTGC AACCTTCTGA AGACCAAGAT GTAAAGGAGA ACCATCAGAC CCAAAATAAG 
AGGAAGGAGC CCAGCTCAAC TGATGAGAAC AATCGACGCA ACCGTCAGAG TTCAGATAAT 
AGGCAAATGG AGGATGAAGA GAGTCGCGTA GAGGAAGGAG GTGTGATCTT TTTCTATTTG 
CCTGAAGGCC ACAAGCCTGG CACTTTTCCT AAAGCACTTG CGGATGGAGG ATCCTATCAT 
GGTGTTGACA ACACAAGTCG CCGCCCACCC CGGAGGGGCT CTTCTGCTTC ACTAGACAGT 
CGAATGAGTA TCTATGATAA TGTTCCTTTC TCGGAAGTGG GAGATGATGA GGACGAAGTA 
GGAGAGGAGG AACCAAAACT GGAGGATGTG TTAGAGCGAG TCAGAGGTCT GCAGCAACTG 
GTCAATGAAT GGCCAGAAAC AGGAATTGGG GAAGAGGAGG AGGATGAAGA GGAAGAGGAA 
GGAGATTCAG ATTCAGCCCT TGACTCTGCA TCTCCTTGTC CATCATCTCC ACTGCAGAAC 
CGCTTAGAAG AGACAGAGAA TGGCAGCGAC CAGGACAGCA CTGGAAACCC ATTAGCAGAA 
AGAGATGAGA CACTCAGCTC ACGTGAAAGA TGTGACTCTG GAGTTGAAGC ATCACTTACT 
CGAGCCAACA GAACTCAGAA ACTGCGCTGG CCAAGTTTCC AGAGCTCCCA CAGGCCAAGT 
CTGTCATCAA CAGTGCTACA AGTTGGATGT CAGTCAGTTC TGCAGATGAA TCTACTCCAG 
AAATACAGCC TTCTCAAACT TACTGCTCTA CTGGAGAGAT ACACGCCCAC CAACAAACAT 
GGCTTCAGCT GGGCTGTTCC TAAGTTTATG AAGCGGATCA AGGTTCGCGA CTACAAGGAC 
AGGAGTGTGT TTGGCGTTCC ACTGCAGGTG ATAGTTCAAA GGAGCGGACA ACCCCTGCCT 
CAGAGCATTC AACAAGCCAT GCGTTACCTG CGGAGCCAAT GCCTCGACCA GGTGGGATTG 
TTCAGGAAAT CAGGGGTGAA ATCGCGAATC CAAGCCCTTC GCCAAATGAA CGAATCATGT 
GGCGCTGTTG GGGGAGGAGT CAACTATGAG GGCCAATTAG CTTATGACGT GGCTGACATG 
CTAAAGCAGT ATTTCCGAGA CCTTCCAGAG CCTCTGCTTA CCAGCAAACT GTCTGATACC 
TTCCTGCAGA TTTATCAATA TGTCCCCAAA GAGCAGCGCC TGCAGGCGGT AAGGGCTGCT 
GTGTTGCTGT TACCAGACGA GAACCGCGAG GCACTGCAGA CTCTGCTGTG CTTCCTGAGT 
GATGTAACAG CCAGCGTGGG TGAAAACCAG ATGACCTGCA CCAACCTGGC CGTGTGTTTG 
GCCCCTTCTC TCTTCCACCT CAACACACTG CGCAGAGAGA GCTCGTCACC CAGGGTCATG 
AATAGAAAGA ACACCCTTGG AAAACCTGAC CAAAGAGACC TGAATGAAAA CCTGGCGGCT 
ACTCATGGTT TAGCGCATAT GATACAGGAG TGCAGAAAAC TCTTCCAGAT CCCAGAAGAG 
ATGTCCCGAT GTAGGAACTC TTACATGGAG CAGGGCTTGA GTCCCATGAG GATGGAGGTG 
TTGGCGGAAA ACTGCGGTTC TTGCAACTAC CAAAGTCTAC TCAAAGACAG TATTGATAGC 
CTGCTTAAAG AAGCCAAGGA CAAATTCAAA GGTTATGACA GCTGCTCGAC TCCTGAAAAC 
GCTGAGCTCG CATACAAGAA GGTTCAGGAC GGCTCTTCTC TGCGGCAGTG GAAAGTTTGT 
GTGGATGTGC CTGCATCGGC TGAGGATGTC CTGCAACGCA TTTTGCGAGA GCAGGAGCGC 
TGGGACGAGG ACCTGCTAGA GTCCAGGATC GTGGAGACAC TGGACCACAA CACTGAAGTC 
TATCAGTATG TCAGAAACTC AATGCCACCC CATCCGCCAA GAGACCACGT GGTACTCAGG 
TCATGGATGA CAGATTTGCC CAAGGGAGTG TGTACTTTAG TGAGCGTGTC AGTGGATCAC 
GAGAGCGCAG CAGTGCTGGG TGTCCGTGCC AATGTTCTGA CATCTCGCTA TTACATCGAG 
CCCTGCGGAG CCAACAAGAG CCGCCTCGCA CACATTTCCA GAATAGACTG CAGGGGTCGT 
TGCCCTGAAT GGTACAATAA GCTATATGGT CACCTATGTG CTGCGGAGGT GGTGCGGATA 
CGAGACACTT TTACCTGTCT GGACAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211480.1
CDS	971..5980
Translation	MPVAIRNRSWEDHVTSPRTGLQYSYDDLDLVCCHARHRRGRCASMPECGHHHLYPQDDIGNGTKKMERILQKFKNETQPNCEDVKSQLLTSRDAVNEKAQLESEQDNRLLSNNVADQDELQHSALGNLQTHNGDADNKKLNKDKVSSAISLSHLVPSAEALDQTNGLGNVLKDGISLNNTVKEDCSELTHPKEETSVENTESMVKVNVGSEKLVYEHMNVTSSITEITNKPPTSDSSADPCDNEEGHCCETSEQAILKCIKTERSSKPMSSSSTSQEDHHIPHTEDANKANLACKDCNMTPEENGDGIFKCADGDTLNVGCINPHACESSQLKECGVIDIMDTWHDCSSKLQTIHEGSCPESGDDSSAQISGDGPEEPTCRGLNTRNCENLTASEDLREPQPEGIYEDHQATQNTAAKDCEMVPKADGSIVKLRTRKSSHEEREKERSRLDSMVLLIMKLDQLDKEIENALSSSLNSTPNLKRRIISDVDLETLDSAGSLSASSQSLNTPLYRSSSSLSSSGPTGTPGARPKNETSAVLSENDRAEIEAKEACTWLRAAGFPQYAQLYEDGQFPIEISSVTRDHDFLDQDAIEALCRRLNTLNKCALMRLEITPQRKRSEDSDEDEPCAISGRWTFQRDSKRWSRMDQLDLDVFSPTAGDTGSFAAFLSQDEHFVRGPLQKEGVNASVESILTDLSEQPEVGSLHSTGSGGRGGSLSTTVPSSPATTISNANTATTTRASSVASVCSSGTSGANEDSMSDGLPSPLERGTFAFDVKPNLISSAAEKSTRSRAKSFLKRMESLRLRTSSNAGSKRKKKGVPGHGRLEISGPVLKEGLDEDKLRRLNCVDIATLDRNANRNRSISYSTQTSSGSAGSSQSEASSGSAVSTPSPVSRARSHSTAAGSSKRGGMYLEGFDPFNFVVLQPSEDQDVKENHQTQNKRKEPSSTDENNRRNRQSSDNRQMEDEESRVEEGGVIFFYLPEGHKPGTFPKALADGGSYHGVDNTSRRPPRRGSSASLDSRMSIYDNVPFSEVGDDEDEVGEEEPKLEDVLERVRGLQQLVNEWPETGIGEEEEDEEEEEGDSDSALDSASPCPSSPLQNRLEETENGSDQDSTGNPLAERDETLSSRERCDSGVEASLTRANRTQKLRWPSFQSSHRPSLSSTVLQVGCQSVLQMNLLQKYSLLKLTALLERYTPTNKHGFSWAVPKFMKRIKVRDYKDRSVFGVPLQVIVQRSGQPLPQSIQQAMRYLRSQCLDQVGLFRKSGVKSRIQALRQMNESCGAVGGGVNYEGQLAYDVADMLKQYFRDLPEPLLTSKLSDTFLQIYQYVPKEQRLQAVRAAVLLLPDENREALQTLLCFLSDVTASVGENQMTCTNLAVCLAPSLFHLNTLRRESSSPRVMNRKNTLGKPDQRDLNENLAATHGLAHMIQECRKLFQIPEEMSRCRNSYMEQGLSPMRMEVLAENCGSCNYQSLLKDSIDSLLKEAKDKFKGYDSCSTPENAELAYKKVQDGSSLRQWKVCVDVPASAEDVLQRILREQERWDEDLLESRIVETLDHNTEVYQYVRNSMPPHPPRDHVVLRSWMTDLPKGVCTLVSVSVDHESAAVLGVRANVLTSRYYIEPCGANKSRLAHISRIDCRGRCPEWYNKLYGHLCAAEVVRIRDTFTCLDK

Target ORF information:

RefSeq Version	XM_017355991.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DLC1 Rho GTPase activating protein (dlc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355991.1

ORF Insert Sequence:

ATGCCTGTTG CCATTCGTAA TAGAAGCTGG GAGGATCACG TGACTTCACC ACGAACAGGA 
TTACAGTACA GCTATGATGA CTTAGACTTG GTTTGCTGCC ATGCAAGGCA CCGGCGAGGT 
AGATGCGCCT CCATGCCAGA ATGTGGCCAT CATCATCTTT ACCCTCAGGA TGATATTGGA 
AATGGTACAA AAAAGATGGA GAGGATATTG CAAAAGTTTA AGAATGAGAC TCAACCTAAC 
TGTGAAGATG TAAAGAGCCA ATTGTTGACA TCTAGAGATG CTGTCAATGA AAAGGCACAG 
CTTGAGAGTG AGCAAGATAA CAGACTACTT TCAAACAACG TGGCAGATCA AGATGAATTA 
CAGCATTCTG CTTTGGGAAA TTTGCAAACT CACAATGGAG ATGCAGATAA TAAGAAGCTA 
AACAAGGATA AAGTGTCTTC AGCCATTAGC TTGTCACATC TTGTGCCTTC TGCTGAAGCG 
TTGGATCAAA CAAATGGTTT AGGAAATGTT TTGAAGGATG GCATCTCCTT AAACAACACA 
GTCAAAGAAG ACTGCTCTGA GCTAACTCAT CCCAAAGAAG AAACATCTGT GGAAAACACA 
GAATCTATGG TGAAGGTCAA TGTGGGCAGT GAGAAGTTAG TATATGAACA CATGAATGTG 
ACAAGCAGCA TCACAGAAAT AACTAATAAA CCTCCCACTT CTGATTCATC TGCTGACCCG 
TGTGATAATG AGGAAGGACA TTGTTGTGAA ACATCTGAAC AGGCAATTTT AAAATGTATT 
AAAACTGAAC GCTCAAGCAA ACCTATGTCA TCATCATCAA CTTCTCAAGA AGATCATCAC 
ATTCCCCACA CAGAAGATGC TAATAAAGCA AATCTAGCAT GCAAAGATTG CAATATGACT 
CCAGAAGAAA ATGGAGATGG TATTTTTAAG TGTGCTGATG GTGATACGTT AAACGTTGGA 
TGCATTAACC CACATGCTTG TGAAAGTTCT CAACTGAAAG AATGTGGAGT AATAGACATC 
ATGGATACAT GGCATGACTG CTCGTCCAAA CTCCAAACCA TCCATGAGGG GTCGTGTCCT 
GAATCTGGAG ATGATTCTTC TGCTCAGATA TCAGGTGATG GGCCAGAGGA ACCAACATGC 
AGAGGATTAA ACACAAGGAA CTGTGAAAAT CTGACAGCCT CAGAGGATCT CAGAGAACCT 
CAACCTGAAG GAATCTATGA GGATCATCAG GCGACTCAAA ACACTGCAGC CAAGGACTGT 
GAGATGGTCC CCAAAGCTGA TGGTAGCATT GTCAAGCTTC GCACAAGAAA GAGCTCACAT 
GAAGAGAGGG AGAAGGAGAG GTCCCGGCTG GACTCAATGG TTCTACTAAT AATGAAGCTG 
GATCAGCTGG ACAAAGAGAT TGAAAATGCT CTAAGCTCCT CCCTAAATAG CACACCCAAT 
TTAAAGAGGC GGATCATTTC TGATGTTGAC TTAGAAACTC TGGATTCAGC CGGAAGTCTT 
TCAGCCTCCT CACAGTCTCT AAACACCCCA CTTTATCGCT CTTCTTCATC GTTGTCTTCA 
TCAGGACCCA CCGGAACCCC TGGAGCAAGA CCAAAAAATG AGACTTCTGC TGTACTTTCT 
GAGAACGACA GAGCAGAAAT TGAGGCGAAG GAGGCGTGTA CATGGCTGAG GGCTGCAGGT 
TTTCCACAGT ATGCTCAGCT TTATGAAGAT GGTCAGTTCC CGATTGAGAT TTCCTCAGTT 
ACCAGAGATC ACGACTTCCT GGATCAAGAT GCCATAGAAG CCTTGTGCAG GAGACTGAAC 
ACCCTAAACA AGTGCGCTCT TATGAGATTA GAGATCACCC CTCAAAGAAA GAGAAGTGAA 
GACTCTGATG AGGATGAGCC ATGTGCCATA AGTGGCCGTT GGACTTTCCA GCGAGACAGC 
AAGCGCTGGT CTCGCATGGA CCAGCTGGAC CTTGATGTAT TCTCCCCTAC TGCAGGGGAC 
ACTGGCTCCT TCGCTGCATT CCTATCCCAA GATGAGCATT TTGTTAGAGG GCCCCTTCAA 
AAGGAGGGTG TTAATGCCAG TGTGGAGAGC ATCTTAACGG ACCTTAGCGA GCAGCCAGAG 
GTGGGATCTC TGCATAGTAC TGGTAGTGGA GGTCGAGGAG GATCACTGAG TACCACTGTA 
CCATCCAGTC CTGCTACAAC CATCTCCAAT GCTAATACAG CCACCACAAC AAGAGCAAGC 
TCAGTTGCCA GTGTCTGTTC TTCTGGAACA TCAGGTGCCA ATGAAGACTC CATGTCTGAT 
GGGCTTCCAT CGCCCCTGGA ACGTGGTACC TTTGCTTTTG ATGTAAAACC AAACTTGATA 
AGTAGTGCAG CTGAAAAAAG CACACGGTCA AGAGCAAAGA GCTTTTTAAA ACGCATGGAA 
AGTTTACGTC TACGCACCAG TAGCAATGCA GGCTCCAAGC GCAAGAAGAA AGGTGTTCCT 
GGGCATGGTA GGTTGGAGAT CAGTGGGCCA GTGCTTAAGG AGGGTCTTGA TGAAGACAAG 
CTGCGTCGGC TCAACTGTGT TGACATCGCA ACTCTGGATC GAAATGCAAA CCGAAATCGA 
AGCATCTCCT ATTCAACGCA AACAAGCAGT GGGAGTGCAG GGAGTAGCCA ATCAGAAGCT 
AGCAGTGGGA GTGCTGTAAG TACGCCCAGT CCAGTTTCAA GAGCACGTAG TCACAGCACA 
GCAGCCGGGT CCAGCAAGAG AGGTGGGATG TACTTGGAAG GTTTTGACCC ATTTAACTTT 
GTTGTGCTGC AACCTTCTGA AGACCAAGAT GTAAAGGAGA ACCATCAGAC CCAAAATAAG 
AGGAAGGAGC CCAGCTCAAC TGATGAGAAC AATCGACGCA ACCGTCAGAG TTCAGATAAT 
AGGCAAATGG AGGATGAAGA GAGTCGCGTA GAGGAAGGAG GTGTGATCTT TTTCTATTTG 
CCTGAAGGCC ACAAGCCTGG CACTTTTCCT AAAGCACTTG CGGATGGAGG ATCCTATCAT 
GGTGTTGACA ACACAAGTCG CCGCCCACCC CGGAGGGGCT CTTCTGCTTC ACTAGACAGT 
CGAATGAGTA TCTATGATAA TGTTCCTTTC TCGGAAGTGG GAGATGATGA GGACGAAGTA 
GGAGAGGAGG AACCAAAACT GGAGGATGTG TTAGAGCGAG TCAGAGGTCT GCAGCAACTG 
GTCAATGAAT GGCCAGAAAC AGGAATTGGG GAAGAGGAGG AGGATGAAGA GGAAGAGGAA 
GGAGATTCAG ATTCAGCCCT TGACTCTGCA TCTCCTTGTC CATCATCTCC ACTGCAGAAC 
CGCTTAGAAG AGACAGAGAA TGGCAGCGAC CAGGACAGCA CTGGAAACCC ATTAGCAGAA 
AGAGATGAGA CACTCAGCTC ACGTGAAAGA TGTGACTCTG GAGTTGAAGC ATCACTTACT 
CGAGCCAACA GAACTCAGAA ACTGCGCTGG CCAAGTTTCC AGAGCTCCCA CAGGCCAAGT 
CTGTCATCAA CAGTGCTACA AGTTGGATGT CAGTCAGTTC TGCAGATGAA TCTACTCCAG 
AAATACAGCC TTCTCAAACT TACTGCTCTA CTGGAGAGAT ACACGCCCAC CAACAAACAT 
GGCTTCAGCT GGGCTGTTCC TAAGTTTATG AAGCGGATCA AGGTTCGCGA CTACAAGGAC 
AGGAGTGTGT TTGGCGTTCC ACTGCAGGTG ATAGTTCAAA GGAGCGGACA ACCCCTGCCT 
CAGAGCATTC AACAAGCCAT GCGTTACCTG CGGAGCCAAT GCCTCGACCA GGTGGGATTG 
TTCAGGAAAT CAGGGGTGAA ATCGCGAATC CAAGCCCTTC GCCAAATGAA CGAATCATGT 
GGCGCTGTTG GGGGAGGAGT CAACTATGAG GGCCAATTAG CTTATGACGT GGCTGACATG 
CTAAAGCAGT ATTTCCGAGA CCTTCCAGAG CCTCTGCTTA CCAGCAAACT GTCTGATACC 
TTCCTGCAGA TTTATCAATA TGTCCCCAAA GAGCAGCGCC TGCAGGCGGT AAGGGCTGCT 
GTGTTGCTGT TACCAGACGA GAACCGCGAG GCACTGCAGA CTCTGCTGTG CTTCCTGAGT 
GATGTAACAG CCAGCGTGGG TGAAAACCAG ATGACCTGCA CCAACCTGGC CGTGTGTTTG 
GCCCCTTCTC TCTTCCACCT CAACACACTG CGCAGAGAGA GCTCGTCACC CAGGGTCATG 
AATAGAAAGA ACACCCTTGG AAAACCTGAC CAAAGAGACC TGAATGAAAA CCTGGCGGCT 
ACTCATGGTT TAGCGCATAT GATACAGGAG TGCAGAAAAC TCTTCCAGAT CCCAGAAGAG 
ATGTCCCGAT GTAGGAACTC TTACATGGAG CAGGGCTTGA GTCCCATGAG GATGGAGGTG 
TTGGCGGAAA ACTGCGGTTC TTGCAACTAC CAAAGTCTAC TCAAAGACAG TATTGATAGC 
CTGCTTAAAG AAGCCAAGGA CAAATTCAAA GGTTATGACA GCTGCTCGAC TCCTGAAAAC 
GCTGAGCTCG CATACAAGAA GGTTCAGGAC GGCTCTTCTC TGCGGCAGTG GAAAGTTTGT 
GTGGATGTGC CTGCATCGGC TGAGGATGTC CTGCAACGCA TTTTGCGAGA GCAGGAGCGC 
TGGGACGAGG ACCTGCTAGA GTCCAGGATC GTGGAGACAC TGGACCACAA CACTGAAGTC 
TATCAGTATG TCAGAAACTC AATGCCACCC CATCCGCCAA GAGACCACGT GGTACTCAGG 
TCATGGATGA CAGATTTGCC CAAGGGAGTG TGTACTTTAG TGAGCGTGTC AGTGGATCAC 
GAGAGCGCAG CAGTGCTGGG TGTCCGTGCC AATGTTCTGA CATCTCGCTA TTACATCGAG 
CCCTGCGGAG CCAACAAGAG CCGCCTCGCA CACATTTCCA GAATAGACTG CAGGGGTCGT 
TGCCCTGAAT GGTACAATAA GCTATATGGT CACCTATGTG CTGCGGAGGT GGTGCGGATA 
CGAGACACTT TTACCTGTCT GGACAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dlc1 ( XM_017355991.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211480.1 Danio rerio DLC1 Rho GTPase activating protein (dlc1), transcript variant X1, mRNA.

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CloneID	ODa53869
Clone ID Related Accession (Same CDS sequence)	XM_001921739.6 , XM_017355992.1 , XM_017355991.1 , XM_017355991.2
Accession Version	XM_017355991.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho GTPase-activating protein 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017355991.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTGTTG CCATTCGTAA TAGAAGCTGG GAGGATCACG TGACTTCACC ACGAACAGGA 
TTACAGTACA GCTATGATGA CTTAGACTTG GTTTGCTGCC ATGCAAGGCA CCGGCGAGGT 
AGATGCGCCT CCATGCCAGA ATGTGGCCAT CATCATCTTT ACCCTCAGGA TGATATTGGA 
AATGGTACAA AAAAGATGGA GAGGATATTG CAAAAGTTTA AGAATGAGAC TCAACCTAAC 
TGTGAAGATG TAAAGAGCCA ATTGTTGACA TCTAGAGATG CTGTCAATGA AAAGGCACAG 
CTTGAGAGTG AGCAAGATAA CAGACTACTT TCAAACAACG TGGCAGATCA AGATGAATTA 
CAGCATTCTG CTTTGGGAAA TTTGCAAACT CACAATGGAG ATGCAGATAA TAAGAAGCTA 
AACAAGGATA AAGTGTCTTC AGCCATTAGC TTGTCACATC TTGTGCCTTC TGCTGAAGCG 
TTGGATCAAA CAAATGGTTT AGGAAATGTT TTGAAGGATG GCATCTCCTT AAACAACACA 
GTCAAAGAAG ACTGCTCTGA GCTAACTCAT CCCAAAGAAG AAACATCTGT GGAAAACACA 
GAATCTATGG TGAAGGTCAA TGTGGGCAGT GAGAAGTTAG TATATGAACA CATGAATGTG 
ACAAGCAGCA TCACAGAAAT AACTAATAAA CCTCCCACTT CTGATTCATC TGCTGACCCG 
TGTGATAATG AGGAAGGACA TTGTTGTGAA ACATCTGAAC AGGCAATTTT AAAATGTATT 
AAAACTGAAC GCTCAAGCAA ACCTATGTCA TCATCATCAA CTTCTCAAGA AGATCATCAC 
ATTCCCCACA CAGAAGATGC TAATAAAGCA AATCTAGCAT GCAAAGATTG CAATATGACT 
CCAGAAGAAA ATGGAGATGG TATTTTTAAG TGTGCTGATG GTGATACGTT AAACGTTGGA 
TGCATTAACC CACATGCTTG TGAAAGTTCT CAACTGAAAG AATGTGGAGT AATAGACATC 
ATGGATACAT GGCATGACTG CTCGTCCAAA CTCCAAACCA TCCATGAGGG GTCGTGTCCT 
GAATCTGGAG ATGATTCTTC TGCTCAGATA TCAGGTGATG GGCCAGAGGA ACCAACATGC 
AGAGGATTAA ACACAAGGAA CTGTGAAAAT CTGACAGCCT CAGAGGATCT CAGAGAACCT 
CAACCTGAAG GAATCTATGA GGATCATCAG GCGACTCAAA ACACTGCAGC CAAGGACTGT 
GAGATGGTCC CCAAAGCTGA TGGTAGCATT GTCAAGCTTC GCACAAGAAA GAGCTCACAT 
GAAGAGAGGG AGAAGGAGAG GTCCCGGCTG GACTCAATGG TTCTACTAAT AATGAAGCTG 
GATCAGCTGG ACAAAGAGAT TGAAAATGCT CTAAGCTCCT CCCTAAATAG CACACCCAAT 
TTAAAGAGGC GGATCATTTC TGATGTTGAC TTAGAAACTC TGGATTCAGC CGGAAGTCTT 
TCAGCCTCCT CACAGTCTCT AAACACCCCA CTTTATCGCT CTTCTTCATC GTTGTCTTCA 
TCAGGACCCA CCGGAACCCC TGGAGCAAGA CCAAAAAATG AGACTTCTGC TGTACTTTCT 
GAGAACGACA GAGCAGAAAT TGAGGCGAAG GAGGCGTGTA CATGGCTGAG GGCTGCAGGT 
TTTCCACAGT ATGCTCAGCT TTATGAAGAT GGTCAGTTCC CGATTGAGAT TTCCTCAGTT 
ACCAGAGATC ACGACTTCCT GGATCAAGAT GCCATAGAAG CCTTGTGCAG GAGACTGAAC 
ACCCTAAACA AGTGCGCTCT TATGAGATTA GAGATCACCC CTCAAAGAAA GAGAAGTGAA 
GACTCTGATG AGGATGAGCC ATGTGCCATA AGTGGCCGTT GGACTTTCCA GCGAGACAGC 
AAGCGCTGGT CTCGCATGGA CCAGCTGGAC CTTGATGTAT TCTCCCCTAC TGCAGGGGAC 
ACTGGCTCCT TCGCTGCATT CCTATCCCAA GATGAGCATT TTGTTAGAGG GCCCCTTCAA 
AAGGAGGGTG TTAATGCCAG TGTGGAGAGC ATCTTAACGG ACCTTAGCGA GCAGCCAGAG 
GTGGGATCTC TGCATAGTAC TGGTAGTGGA GGTCGAGGAG GATCACTGAG TACCACTGTA 
CCATCCAGTC CTGCTACAAC CATCTCCAAT GCTAATACAG CCACCACAAC AAGAGCAAGC 
TCAGTTGCCA GTGTCTGTTC TTCTGGAACA TCAGGTGCCA ATGAAGACTC CATGTCTGAT 
GGGCTTCCAT CGCCCCTGGA ACGTGGTACC TTTGCTTTTG ATGTAAAACC AAACTTGATA 
AGTAGTGCAG CTGAAAAAAG CACACGGTCA AGAGCAAAGA GCTTTTTAAA ACGCATGGAA 
AGTTTACGTC TACGCACCAG TAGCAATGCA GGCTCCAAGC GCAAGAAGAA AGGTGTTCCT 
GGGCATGGTA GGTTGGAGAT CAGTGGGCCA GTGCTTAAGG AGGGTCTTGA TGAAGACAAG 
CTGCGTCGGC TCAACTGTGT TGACATCGCA ACTCTGGATC GAAATGCAAA CCGAAATCGA 
AGCATCTCCT ATTCAACGCA AACAAGCAGT GGGAGTGCAG GGAGTAGCCA ATCAGAAGCT 
AGCAGTGGGA GTGCTGTAAG TACGCCCAGT CCAGTTTCAA GAGCACGTAG TCACAGCACA 
GCAGCCGGGT CCAGCAAGAG AGGTGGGATG TACTTGGAAG GTTTTGACCC ATTTAACTTT 
GTTGTGCTGC AACCTTCTGA AGACCAAGAT GTAAAGGAGA ACCATCAGAC CCAAAATAAG 
AGGAAGGAGC CCAGCTCAAC TGATGAGAAC AATCGACGCA ACCGTCAGAG TTCAGATAAT 
AGGCAAATGG AGGATGAAGA GAGTCGCGTA GAGGAAGGAG GTGTGATCTT TTTCTATTTG 
CCTGAAGGCC ACAAGCCTGG CACTTTTCCT AAAGCACTTG CGGATGGAGG ATCCTATCAT 
GGTGTTGACA ACACAAGTCG CCGCCCACCC CGGAGGGGCT CTTCTGCTTC ACTAGACAGT 
CGAATGAGTA TCTATGATAA TGTTCCTTTC TCGGAAGTGG GAGATGATGA GGACGAAGTA 
GGAGAGGAGG AACCAAAACT GGAGGATGTG TTAGAGCGAG TCAGAGGTCT GCAGCAACTG 
GTCAATGAAT GGCCAGAAAC AGGAATTGGG GAAGAGGAGG AGGATGAAGA GGAAGAGGAA 
GGAGATTCAG ATTCAGCCCT TGACTCTGCA TCTCCTTGTC CATCATCTCC ACTGCAGAAC 
CGCTTAGAAG AGACAGAGAA TGGCAGCGAC CAGGACAGCA CTGGAAACCC ATTAGCAGAA 
AGAGATGAGA CACTCAGCTC ACGTGAAAGA TGTGACTCTG GAGTTGAAGC ATCACTTACT 
CGAGCCAACA GAACTCAGAA ACTGCGCTGG CCAAGTTTCC AGAGCTCCCA CAGGCCAAGT 
CTGTCATCAA CAGTGCTACA AGTTGGATGT CAGTCAGTTC TGCAGATGAA TCTACTCCAG 
AAATACAGCC TTCTCAAACT TACTGCTCTA CTGGAGAGAT ACACGCCCAC CAACAAACAT 
GGCTTCAGCT GGGCTGTTCC TAAGTTTATG AAGCGGATCA AGGTTCGCGA CTACAAGGAC 
AGGAGTGTGT TTGGCGTTCC ACTGCAGGTG ATAGTTCAAA GGAGCGGACA ACCCCTGCCT 
CAGAGCATTC AACAAGCCAT GCGTTACCTG CGGAGCCAAT GCCTCGACCA GGTGGGATTG 
TTCAGGAAAT CAGGGGTGAA ATCGCGAATC CAAGCCCTTC GCCAAATGAA CGAATCATGT 
GGCGCTGTTG GGGGAGGAGT CAACTATGAG GGCCAATTAG CTTATGACGT GGCTGACATG 
CTAAAGCAGT ATTTCCGAGA CCTTCCAGAG CCTCTGCTTA CCAGCAAACT GTCTGATACC 
TTCCTGCAGA TTTATCAATA TGTCCCCAAA GAGCAGCGCC TGCAGGCGGT AAGGGCTGCT 
GTGTTGCTGT TACCAGACGA GAACCGCGAG GCACTGCAGA CTCTGCTGTG CTTCCTGAGT 
GATGTAACAG CCAGCGTGGG TGAAAACCAG ATGACCTGCA CCAACCTGGC CGTGTGTTTG 
GCCCCTTCTC TCTTCCACCT CAACACACTG CGCAGAGAGA GCTCGTCACC CAGGGTCATG 
AATAGAAAGA ACACCCTTGG AAAACCTGAC CAAAGAGACC TGAATGAAAA CCTGGCGGCT 
ACTCATGGTT TAGCGCATAT GATACAGGAG TGCAGAAAAC TCTTCCAGAT CCCAGAAGAG 
ATGTCCCGAT GTAGGAACTC TTACATGGAG CAGGGCTTGA GTCCCATGAG GATGGAGGTG 
TTGGCGGAAA ACTGCGGTTC TTGCAACTAC CAAAGTCTAC TCAAAGACAG TATTGATAGC 
CTGCTTAAAG AAGCCAAGGA CAAATTCAAA GGTTATGACA GCTGCTCGAC TCCTGAAAAC 
GCTGAGCTCG CATACAAGAA GGTTCAGGAC GGCTCTTCTC TGCGGCAGTG GAAAGTTTGT 
GTGGATGTGC CTGCATCGGC TGAGGATGTC CTGCAACGCA TTTTGCGAGA GCAGGAGCGC 
TGGGACGAGG ACCTGCTAGA GTCCAGGATC GTGGAGACAC TGGACCACAA CACTGAAGTC 
TATCAGTATG TCAGAAACTC AATGCCACCC CATCCGCCAA GAGACCACGT GGTACTCAGG 
TCATGGATGA CAGATTTGCC CAAGGGAGTG TGTACTTTAG TGAGCGTGTC AGTGGATCAC 
GAGAGCGCAG CAGTGCTGGG TGTCCGTGCC AATGTTCTGA CATCTCGCTA TTACATCGAG 
CCCTGCGGAG CCAACAAGAG CCGCCTCGCA CACATTTCCA GAATAGACTG CAGGGGTCGT 
TGCCCTGAAT GGTACAATAA GCTATATGGT CACCTATGTG CTGCGGAGGT GGTGCGGATA 
CGAGACACTT TTACCTGTCT GGACAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211480.1
CDS	984..5993
Translation	MPVAIRNRSWEDHVTSPRTGLQYSYDDLDLVCCHARHRRGRCASMPECGHHHLYPQDDIGNGTKKMERILQKFKNETQPNCEDVKSQLLTSRDAVNEKAQLESEQDNRLLSNNVADQDELQHSALGNLQTHNGDADNKKLNKDKVSSAISLSHLVPSAEALDQTNGLGNVLKDGISLNNTVKEDCSELTHPKEETSVENTESMVKVNVGSEKLVYEHMNVTSSITEITNKPPTSDSSADPCDNEEGHCCETSEQAILKCIKTERSSKPMSSSSTSQEDHHIPHTEDANKANLACKDCNMTPEENGDGIFKCADGDTLNVGCINPHACESSQLKECGVIDIMDTWHDCSSKLQTIHEGSCPESGDDSSAQISGDGPEEPTCRGLNTRNCENLTASEDLREPQPEGIYEDHQATQNTAAKDCEMVPKADGSIVKLRTRKSSHEEREKERSRLDSMVLLIMKLDQLDKEIENALSSSLNSTPNLKRRIISDVDLETLDSAGSLSASSQSLNTPLYRSSSSLSSSGPTGTPGARPKNETSAVLSENDRAEIEAKEACTWLRAAGFPQYAQLYEDGQFPIEISSVTRDHDFLDQDAIEALCRRLNTLNKCALMRLEITPQRKRSEDSDEDEPCAISGRWTFQRDSKRWSRMDQLDLDVFSPTAGDTGSFAAFLSQDEHFVRGPLQKEGVNASVESILTDLSEQPEVGSLHSTGSGGRGGSLSTTVPSSPATTISNANTATTTRASSVASVCSSGTSGANEDSMSDGLPSPLERGTFAFDVKPNLISSAAEKSTRSRAKSFLKRMESLRLRTSSNAGSKRKKKGVPGHGRLEISGPVLKEGLDEDKLRRLNCVDIATLDRNANRNRSISYSTQTSSGSAGSSQSEASSGSAVSTPSPVSRARSHSTAAGSSKRGGMYLEGFDPFNFVVLQPSEDQDVKENHQTQNKRKEPSSTDENNRRNRQSSDNRQMEDEESRVEEGGVIFFYLPEGHKPGTFPKALADGGSYHGVDNTSRRPPRRGSSASLDSRMSIYDNVPFSEVGDDEDEVGEEEPKLEDVLERVRGLQQLVNEWPETGIGEEEEDEEEEEGDSDSALDSASPCPSSPLQNRLEETENGSDQDSTGNPLAERDETLSSRERCDSGVEASLTRANRTQKLRWPSFQSSHRPSLSSTVLQVGCQSVLQMNLLQKYSLLKLTALLERYTPTNKHGFSWAVPKFMKRIKVRDYKDRSVFGVPLQVIVQRSGQPLPQSIQQAMRYLRSQCLDQVGLFRKSGVKSRIQALRQMNESCGAVGGGVNYEGQLAYDVADMLKQYFRDLPEPLLTSKLSDTFLQIYQYVPKEQRLQAVRAAVLLLPDENREALQTLLCFLSDVTASVGENQMTCTNLAVCLAPSLFHLNTLRRESSSPRVMNRKNTLGKPDQRDLNENLAATHGLAHMIQECRKLFQIPEEMSRCRNSYMEQGLSPMRMEVLAENCGSCNYQSLLKDSIDSLLKEAKDKFKGYDSCSTPENAELAYKKVQDGSSLRQWKVCVDVPASAEDVLQRILREQERWDEDLLESRIVETLDHNTEVYQYVRNSMPPHPPRDHVVLRSWMTDLPKGVCTLVSVSVDHESAAVLGVRANVLTSRYYIEPCGANKSRLAHISRIDCRGRCPEWYNKLYGHLCAAEVVRIRDTFTCLDK

Target ORF information:

RefSeq Version	XM_017355991.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DLC1 Rho GTPase activating protein (dlc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355991.2

ORF Insert Sequence:

ATGCCTGTTG CCATTCGTAA TAGAAGCTGG GAGGATCACG TGACTTCACC ACGAACAGGA 
TTACAGTACA GCTATGATGA CTTAGACTTG GTTTGCTGCC ATGCAAGGCA CCGGCGAGGT 
AGATGCGCCT CCATGCCAGA ATGTGGCCAT CATCATCTTT ACCCTCAGGA TGATATTGGA 
AATGGTACAA AAAAGATGGA GAGGATATTG CAAAAGTTTA AGAATGAGAC TCAACCTAAC 
TGTGAAGATG TAAAGAGCCA ATTGTTGACA TCTAGAGATG CTGTCAATGA AAAGGCACAG 
CTTGAGAGTG AGCAAGATAA CAGACTACTT TCAAACAACG TGGCAGATCA AGATGAATTA 
CAGCATTCTG CTTTGGGAAA TTTGCAAACT CACAATGGAG ATGCAGATAA TAAGAAGCTA 
AACAAGGATA AAGTGTCTTC AGCCATTAGC TTGTCACATC TTGTGCCTTC TGCTGAAGCG 
TTGGATCAAA CAAATGGTTT AGGAAATGTT TTGAAGGATG GCATCTCCTT AAACAACACA 
GTCAAAGAAG ACTGCTCTGA GCTAACTCAT CCCAAAGAAG AAACATCTGT GGAAAACACA 
GAATCTATGG TGAAGGTCAA TGTGGGCAGT GAGAAGTTAG TATATGAACA CATGAATGTG 
ACAAGCAGCA TCACAGAAAT AACTAATAAA CCTCCCACTT CTGATTCATC TGCTGACCCG 
TGTGATAATG AGGAAGGACA TTGTTGTGAA ACATCTGAAC AGGCAATTTT AAAATGTATT 
AAAACTGAAC GCTCAAGCAA ACCTATGTCA TCATCATCAA CTTCTCAAGA AGATCATCAC 
ATTCCCCACA CAGAAGATGC TAATAAAGCA AATCTAGCAT GCAAAGATTG CAATATGACT 
CCAGAAGAAA ATGGAGATGG TATTTTTAAG TGTGCTGATG GTGATACGTT AAACGTTGGA 
TGCATTAACC CACATGCTTG TGAAAGTTCT CAACTGAAAG AATGTGGAGT AATAGACATC 
ATGGATACAT GGCATGACTG CTCGTCCAAA CTCCAAACCA TCCATGAGGG GTCGTGTCCT 
GAATCTGGAG ATGATTCTTC TGCTCAGATA TCAGGTGATG GGCCAGAGGA ACCAACATGC 
AGAGGATTAA ACACAAGGAA CTGTGAAAAT CTGACAGCCT CAGAGGATCT CAGAGAACCT 
CAACCTGAAG GAATCTATGA GGATCATCAG GCGACTCAAA ACACTGCAGC CAAGGACTGT 
GAGATGGTCC CCAAAGCTGA TGGTAGCATT GTCAAGCTTC GCACAAGAAA GAGCTCACAT 
GAAGAGAGGG AGAAGGAGAG GTCCCGGCTG GACTCAATGG TTCTACTAAT AATGAAGCTG 
GATCAGCTGG ACAAAGAGAT TGAAAATGCT CTAAGCTCCT CCCTAAATAG CACACCCAAT 
TTAAAGAGGC GGATCATTTC TGATGTTGAC TTAGAAACTC TGGATTCAGC CGGAAGTCTT 
TCAGCCTCCT CACAGTCTCT AAACACCCCA CTTTATCGCT CTTCTTCATC GTTGTCTTCA 
TCAGGACCCA CCGGAACCCC TGGAGCAAGA CCAAAAAATG AGACTTCTGC TGTACTTTCT 
GAGAACGACA GAGCAGAAAT TGAGGCGAAG GAGGCGTGTA CATGGCTGAG GGCTGCAGGT 
TTTCCACAGT ATGCTCAGCT TTATGAAGAT GGTCAGTTCC CGATTGAGAT TTCCTCAGTT 
ACCAGAGATC ACGACTTCCT GGATCAAGAT GCCATAGAAG CCTTGTGCAG GAGACTGAAC 
ACCCTAAACA AGTGCGCTCT TATGAGATTA GAGATCACCC CTCAAAGAAA GAGAAGTGAA 
GACTCTGATG AGGATGAGCC ATGTGCCATA AGTGGCCGTT GGACTTTCCA GCGAGACAGC 
AAGCGCTGGT CTCGCATGGA CCAGCTGGAC CTTGATGTAT TCTCCCCTAC TGCAGGGGAC 
ACTGGCTCCT TCGCTGCATT CCTATCCCAA GATGAGCATT TTGTTAGAGG GCCCCTTCAA 
AAGGAGGGTG TTAATGCCAG TGTGGAGAGC ATCTTAACGG ACCTTAGCGA GCAGCCAGAG 
GTGGGATCTC TGCATAGTAC TGGTAGTGGA GGTCGAGGAG GATCACTGAG TACCACTGTA 
CCATCCAGTC CTGCTACAAC CATCTCCAAT GCTAATACAG CCACCACAAC AAGAGCAAGC 
TCAGTTGCCA GTGTCTGTTC TTCTGGAACA TCAGGTGCCA ATGAAGACTC CATGTCTGAT 
GGGCTTCCAT CGCCCCTGGA ACGTGGTACC TTTGCTTTTG ATGTAAAACC AAACTTGATA 
AGTAGTGCAG CTGAAAAAAG CACACGGTCA AGAGCAAAGA GCTTTTTAAA ACGCATGGAA 
AGTTTACGTC TACGCACCAG TAGCAATGCA GGCTCCAAGC GCAAGAAGAA AGGTGTTCCT 
GGGCATGGTA GGTTGGAGAT CAGTGGGCCA GTGCTTAAGG AGGGTCTTGA TGAAGACAAG 
CTGCGTCGGC TCAACTGTGT TGACATCGCA ACTCTGGATC GAAATGCAAA CCGAAATCGA 
AGCATCTCCT ATTCAACGCA AACAAGCAGT GGGAGTGCAG GGAGTAGCCA ATCAGAAGCT 
AGCAGTGGGA GTGCTGTAAG TACGCCCAGT CCAGTTTCAA GAGCACGTAG TCACAGCACA 
GCAGCCGGGT CCAGCAAGAG AGGTGGGATG TACTTGGAAG GTTTTGACCC ATTTAACTTT 
GTTGTGCTGC AACCTTCTGA AGACCAAGAT GTAAAGGAGA ACCATCAGAC CCAAAATAAG 
AGGAAGGAGC CCAGCTCAAC TGATGAGAAC AATCGACGCA ACCGTCAGAG TTCAGATAAT 
AGGCAAATGG AGGATGAAGA GAGTCGCGTA GAGGAAGGAG GTGTGATCTT TTTCTATTTG 
CCTGAAGGCC ACAAGCCTGG CACTTTTCCT AAAGCACTTG CGGATGGAGG ATCCTATCAT 
GGTGTTGACA ACACAAGTCG CCGCCCACCC CGGAGGGGCT CTTCTGCTTC ACTAGACAGT 
CGAATGAGTA TCTATGATAA TGTTCCTTTC TCGGAAGTGG GAGATGATGA GGACGAAGTA 
GGAGAGGAGG AACCAAAACT GGAGGATGTG TTAGAGCGAG TCAGAGGTCT GCAGCAACTG 
GTCAATGAAT GGCCAGAAAC AGGAATTGGG GAAGAGGAGG AGGATGAAGA GGAAGAGGAA 
GGAGATTCAG ATTCAGCCCT TGACTCTGCA TCTCCTTGTC CATCATCTCC ACTGCAGAAC 
CGCTTAGAAG AGACAGAGAA TGGCAGCGAC CAGGACAGCA CTGGAAACCC ATTAGCAGAA 
AGAGATGAGA CACTCAGCTC ACGTGAAAGA TGTGACTCTG GAGTTGAAGC ATCACTTACT 
CGAGCCAACA GAACTCAGAA ACTGCGCTGG CCAAGTTTCC AGAGCTCCCA CAGGCCAAGT 
CTGTCATCAA CAGTGCTACA AGTTGGATGT CAGTCAGTTC TGCAGATGAA TCTACTCCAG 
AAATACAGCC TTCTCAAACT TACTGCTCTA CTGGAGAGAT ACACGCCCAC CAACAAACAT 
GGCTTCAGCT GGGCTGTTCC TAAGTTTATG AAGCGGATCA AGGTTCGCGA CTACAAGGAC 
AGGAGTGTGT TTGGCGTTCC ACTGCAGGTG ATAGTTCAAA GGAGCGGACA ACCCCTGCCT 
CAGAGCATTC AACAAGCCAT GCGTTACCTG CGGAGCCAAT GCCTCGACCA GGTGGGATTG 
TTCAGGAAAT CAGGGGTGAA ATCGCGAATC CAAGCCCTTC GCCAAATGAA CGAATCATGT 
GGCGCTGTTG GGGGAGGAGT CAACTATGAG GGCCAATTAG CTTATGACGT GGCTGACATG 
CTAAAGCAGT ATTTCCGAGA CCTTCCAGAG CCTCTGCTTA CCAGCAAACT GTCTGATACC 
TTCCTGCAGA TTTATCAATA TGTCCCCAAA GAGCAGCGCC TGCAGGCGGT AAGGGCTGCT 
GTGTTGCTGT TACCAGACGA GAACCGCGAG GCACTGCAGA CTCTGCTGTG CTTCCTGAGT 
GATGTAACAG CCAGCGTGGG TGAAAACCAG ATGACCTGCA CCAACCTGGC CGTGTGTTTG 
GCCCCTTCTC TCTTCCACCT CAACACACTG CGCAGAGAGA GCTCGTCACC CAGGGTCATG 
AATAGAAAGA ACACCCTTGG AAAACCTGAC CAAAGAGACC TGAATGAAAA CCTGGCGGCT 
ACTCATGGTT TAGCGCATAT GATACAGGAG TGCAGAAAAC TCTTCCAGAT CCCAGAAGAG 
ATGTCCCGAT GTAGGAACTC TTACATGGAG CAGGGCTTGA GTCCCATGAG GATGGAGGTG 
TTGGCGGAAA ACTGCGGTTC TTGCAACTAC CAAAGTCTAC TCAAAGACAG TATTGATAGC 
CTGCTTAAAG AAGCCAAGGA CAAATTCAAA GGTTATGACA GCTGCTCGAC TCCTGAAAAC 
GCTGAGCTCG CATACAAGAA GGTTCAGGAC GGCTCTTCTC TGCGGCAGTG GAAAGTTTGT 
GTGGATGTGC CTGCATCGGC TGAGGATGTC CTGCAACGCA TTTTGCGAGA GCAGGAGCGC 
TGGGACGAGG ACCTGCTAGA GTCCAGGATC GTGGAGACAC TGGACCACAA CACTGAAGTC 
TATCAGTATG TCAGAAACTC AATGCCACCC CATCCGCCAA GAGACCACGT GGTACTCAGG 
TCATGGATGA CAGATTTGCC CAAGGGAGTG TGTACTTTAG TGAGCGTGTC AGTGGATCAC 
GAGAGCGCAG CAGTGCTGGG TGTCCGTGCC AATGTTCTGA CATCTCGCTA TTACATCGAG 
CCCTGCGGAG CCAACAAGAG CCGCCTCGCA CACATTTCCA GAATAGACTG CAGGGGTCGT 
TGCCCTGAAT GGTACAATAA GCTATATGGT CACCTATGTG CTGCGGAGGT GGTGCGGATA 
CGAGACACTT TTACCTGTCT GGACAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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dlc1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

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サポート

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