itpr1a gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_021474618.1	XP_021330293.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X14
XM_009302587.3	XP_009300862.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X3
XM_005166095.3	XP_005166152.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X1
XM_017356666.1	XP_017212155.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X7
XM_017356666.2	XP_017212155.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X8
XM_021474614.1	XP_021330289.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X1
XM_009302585.2	XP_009300860.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X2
XM_005166095.4	XP_005166152.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X1
XM_009302588.3	XP_009300863.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X5
XM_017356665.2	XP_017212154.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X6
XM_021474615.1	XP_021330290.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X11
XM_021474619.1	XP_021330294.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X15
XM_017356665.1	XP_017212154.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X6
XM_009302587.2	XP_009300862.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X3
XM_009302585.3	XP_009300860.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X2
XM_017356664.2	XP_017212153.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X4
XM_021477224.1	XP_021332899.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X7
XM_021474612.1	XP_021330287.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X9
XM_021474613.1	XP_021330288.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X10
XM_009302588.2	XP_009300863.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X5
XM_017356664.1	XP_017212153.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X4
XM_021474616.1	XP_021330291.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X12
XM_021474617.1	XP_021330292.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X13
XM_021474620.1	XP_021330295.1	inositol 1,4,5-trisphosphate receptor type 1 isoform X16

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa143103	XM_021477224.1 Latest version!	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa146878	XM_021474619.1 Latest version!	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa12117	XM_005166095.4 Latest version!	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa146871	XM_021474612.1 Latest version!	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa146872	XM_021474613.1 Latest version!	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa146873	XM_021474614.1 Latest version!	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa146874	XM_021474615.1 Latest version!	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa146875	XM_021474616.1 Latest version!	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa146876	XM_021474617.1 Latest version!	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa146877	XM_021474618.1 Latest version!	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa146879	XM_021474620.1 Latest version!	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa45192	XM_009302585.3 Latest version!	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa45194	XM_009302587.3 Latest version!	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa45195	XM_009302588.3 Latest version!	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa59493	XM_017356664.2 Latest version!	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa59494	XM_017356665.2 Latest version!	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa59495	XM_017356666.2 Latest version!	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
ODa59495	XM_017356666.1	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa12117	XM_005166095.3	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa59494	XM_017356665.1	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa45194	XM_009302587.2	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa45192	XM_009302585.2	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa45195	XM_009302588.2	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa59493	XM_017356664.1	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

itpr1a ( XM_021477224.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332899.1 Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa143103
Clone ID Related Accession (Same CDS sequence)	XM_021477224.1
Accession Version	XM_021477224.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6498bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GGTAAATCCA 
GACTATGAAG AAGAATGCCT GGAGTCCCGC TCCTCTATGG ATTCGGAGCA TGAAGTGATT 
CGGGCTCGTG CACGTAACCC TCAGGATAAG GTGATGTACA CACTGGTGTC TGTTCCTGAT 
GGAAATGACA TATCCTCCAT CTTTGAGCTT GACCCTACAA CTTTGAGGGG CGGAGACTCA 
CTTGTGCCAA GAAACTCGTA TGTGAGGCTC AGGCATCTCT GCACCAACAC GTGGGTCCAC 
AGCACCAACC AGCCAATAGA TAAAGAGGAA GAGAAACCGG TCATGCTCAG AATAGGAACG 
TCTCCTCTGA AGGAAGATAA AGAGGCGTTT GCTATAGTAC CAGTCTCTCC AGCTGAGGTT 
CGAGATCTGG ATTTTGCTAA TGATGCTAGT AAAGTGTTGG CTTCCATTGC TGCCAAACTG 
GAGAAGGGCA CCATTACCCA GAATGAGAGG AGGTCAGTGA CGAAGCTGTT GGAGGATCTG 
GTGTATTTTG TGGTGGACAT ACCCAGCAGT GCTCAAGATG TGCTGGAGAT CACAGTCAAC 
AAACCCAACA GAGAGCGACA GAAACTCATG AGAGAGCAGA ACATTCTTAA ACAGATCTTC 
AAGCTGCTGC AGGCGCCATT TACAGACAGC GGGGACGGCC CTATGTTGCG ATTAGAAGAG 
CTCGCAGACC AGCGTCATGC ACCCTTTCGA CACATTTGCA GACTCTGTTA CAGAGTCCTA 
CGTTACTCAC AACAGGACTA TCGCAAAAAT CAGGAGTACA TAGCAAAGCA GTTCCGCTTC 
ATGCAGAAGC AGATAGGATA TGATGTGCTG GCTGAAGACA CAATTACAGC CCTTCTGCAC 
AACAACAGGA AGCTCCTAGA GAAACACATC ACAGCAGCTG AAATAGATAC CTTTGTCAGT 
CTGGTCCGCA AGAACAGAGA GCCCAGATTT CTGGATTACT TGTCAGATCT GTGTGTGTCG 
ATGAATAAAT CCATCCCGGT CACTCAAGAG CTCATCTGTA ATGCTGTGCT GGACCCAGCC 
AATGCAGACA TTCTCATCGA AACCAAGCTT GTGTTGTCAC GGTTTGAGGT GGCAGGAACG 
GTGTTGGGTG AAGGTGCGGA GGAGGAAGAG GAGGATGAAG AGGAGGTCTG GCTGTTCTGG 
AAGGACAGTG GAGGAGAGGT GAAGAGTAAA AGCATACGAG AGCTTGCACA GGACGCAAAA 
GATGGACATA CAGAGGACCA GGAAGTCATC AATTATTACA GATATCAGCT GAATTTGTTT 
GCTCGGATGT GTCTGGATCG ACAGTATCTT GCGATTAACA AGATATCAGC ACAGCTGGAT 
GTGGATCTGA TTCTGCGCTG CATGTCAGAT GAAGATTTGC CCTTTGACCT CAGAGCATCG 
TTCTGCCGCA TGATGCTGCA CATGCATGTA GACCGTGACC CTCAGGAGCA GGTCACCCCG 
GTCAAATATG CTCGACTGTG GTCTGAAATC CCATCACAAA TTTCCATTGA TGATTATGAT 
AATGATGGGA CGTCAAGTGA TGAAGTGAAA GAGCGCTTCT CTCAGACGAT GGAGTTTGTG 
GAGAATTACC TGAGGGATGT TGTCTGCCAG AGTTTCCCGT TCTCTGATAA GGAGAAAAAC 
AAACTCACTT TTGAGGTGGT GAACCTGGCA AGAAACCTGA TCTATTTTGG TTTCTATAAC 
TTCAGTGATC TGCTAAGGTT AACAAAAATC CTATTGGCAA TCCTGGACTG TGTCCACATA 
AGCACTCCCT TCCACATCAA GCTGGATAGA GAACCCGGTA AAGGAAGTAA CGTGATGCGC 
TCCATCCATG GGGTGGGAGA GCTGATGACA CAGGTGGTGT TGAGGGGTGG GGGATTTCTG 
CCAGCCTCCA GCCACAATCC TCCTGACAGA GACGAAGTGA AAGCACAAAG TGAACCTCAG 
AAACAGGACA TACTGGTGAT GGACACTAAA CTCAAGATCA TCGAGATCCT GCAGTTTATT 
CTGAATGTGC GGTTGGACTA CAGGATCTCC TGCCTCCTCT GTATCTTTAA GACAGAGTTT 
GATGAAAGTA ACTCTCAAAG TGAACCATCT TTAAGCCCAG AATCTCCTGC CAGCGTTCAA 
GGTGCGCTAG ACTTTGAGCA CATTGAAGAG CAGGCGGAGG GTATTTTTGG AGGAAGTAGT 
GAAGAGAACA CCCCGCTGGA TCTTGATGAT CATGGTGGAC GGACATTTCT GAGGGTCCTG 
CTTCACCTGA CCATGCATGA CTATCCTCCG CTGGTATCTC GAGCTCTTCA CCTGCTCTTC 
AGACACTTTA GCCAACGCCA GGAGGTCCTA CAGGCCTTCA AACAGGTTCA GCTCCTGGTG 
ACCAGTCAGG ATGTGGAAAA CTATAAACAG ATCAAGTCAG ATTTGGACCA GCTGAGGTCA 
ATAGTAGAGA AATCAGAGTT GTGGGTGTAT AAGAGACAAG GCTCAGACTC AGGACTGGAT 
GCTGGAGAAG TCACCCCTGA AGCCCACCAC AAGGGTGTCA TCAACTCGAG TAGATCAAAC 
AAACCCAAAG TGGAAAGTAC CAGCAGCTCG AACTACAGAG TCGTGAAAGA GATCCTGCTG 
AGGCTGAGCA AACTGTGTGT TTTGGAGGGC ATCTCTGGCA AGAAGAATAA GAAACAGCAG 
CAGCGGCTCC TCAGGAACAT GGGAGCTCAC AGTGTGGTCC TCGAACTGTT GCAAATCCCT 
TATGAGAAGG GGGAGGATGT CCAGATGCAG GAGATCATGA CCTTAGCACA TCAGTTCCTT 
CAAAACTTCT GTGCTGGAAA TCAGCAGAAC CAGGCACTTC TACACAAACA CATCAACCTG 
TTCCTCAACC CTGGGATCCT GGAGGCGGTG ACCATGCAGC ATATCTTCAT GAATAATTTC 
CAGTTGTGTA GTGAGATTAA TGAGCGGGTC GTGCAGCATT TTGTGCACTG CATCGAGACA 
CACGGCCGCA GCGTGCACTA CCTCAAGTTC CTGCAGACCA TTGTCAAGGC AGAGAACAAG 
TTCATCAAGA AATGCCAAGA CATCGTCATG GCTGAGCTGG TGACTGCAGG TGAGGATGTG 
CTGGTGTTCT ATAATGACCG GGCGTCTTTT CAGTCATTGG TTCAGATGAT GCGTCTTGAG 
CGGGAACGAC TTGATGAGAG CAGTGCACTC AGGTATCACA TTCACCTGGT GGAGTTGCTG 
GCTGTGTGCA CTGAAGGCAA GAACGTCTAC ACTGAGATCA AATGCAACTC GCTGCTCCCT 
CTCGATGATA TTGTACGTGT GGTCACACAT GAGGACTGCA TCCCTGAGGT GAAGATGGCC 
TACGTGAACT TCCTGAATCA CTGTTATGTT GATACAGAGG TTGAGATGAA GGAGATCTAC 
ACCAGCAATC ACATGTGGAA ACTTTTTGAT GATTTTCTGG TGGACGTCTG CAGAGTGTGC 
AATAACACAA GTGACCGTAA ACACGCTGAC ACAGTTCTGG AGAGATACGT TACAGAGACC 
ATAATGAGCA TCGTCACCAC CTTTTTCAGC TCACCATTCT CAGACCAGAG CACAAGTTTG 
CAGACCCGGC AGCCGGTGTT TGTGCAGCTG CTGCAGGGAG TGTTTCGTGT ATATCACTGC 
AACTGGCTTC TCCCAGGTCA GAAGGGGAAT GTGGAAGCCT GCATCAAAGT GCTGAGTGAC 
GTGGCCAAAG GCAGAGCCAT AGCCATTCCT GTGGATCTGG ACTGTCAGGT GAATAATCTG 
TTCATGAAGT CCAACAACAT TGTGCAGAAA ACAGCCCTCA GCTGGAGACT CTCTGTCCGT 
AACGCGACTC GGAGAGACTC TGTTCTGACC ACCTCTCGAG ACTATCGCAA CATCATTGAA 
AGACTACAGG ATATAGTGTC TGCATTGGAG GAGCGGTTGA GGCCTCTGGT TCAGGCTGAG 
TTGTCAGTGT TGGTGGATGT TCTCCATCGC CCAGAGCTGC TGTTTCCAGA GCACACTGAA 
GCCCACCGCA AGTGTGAGAG TGGAGGCTTC ATCTGCAAGT TGATAAAACA CACTAAGCAG 
CTTCTAGAAG AAAACGAAGA GAGGCTTTGC ATCAAAGTCC TTCAGACACT CCGAGAGATG 
ATGACCAAAG ACAGAGGATA TGGAGAGAAG CTTCTGGGAT ATGATGATGA GATGGATGTC 
ACGGAGGTGG TGGATATCAG TCTGCCACCC AAACAGCAGG AGGATCGGCA GAGGGGGGAG 
GCCCTTCGGC AGCTGCTCGT CAATCGTTAC TATGGCTTCA GGAGTGGTGG GCGGAGAGAA 
AGTCTGACCT CGTTTGGTAA CTCCACCCTA ACTCCTGTCG GGCCAATCAA GAGCCAGCCA 
GGGGGGTTGA GCAGGGCTGA GATGTCATTA ATGGAGGTGC AGTGTCATCT AGACCGTGAA 
GGGGCATCGG ATCTTGTCAT TGATCTCATC ATGAACACTA CCAGCGACCG CGTCTTCCAT 
GAAAGCATTC TGTTGGCCAT CGCACTGTTG GAGGGCGGAA ACACCATCAT ACAGCATTCC 
TTCTTCAAAC GTTTAACAGA AGACAAAAAT TCAGAGAAGT TTTTCAGGGT GTTTTATGAC 
AGAATGAAAG TGGCTCAGGT GGAGATCAAA GCCACGGTTA CTGTCAACAC TAGTGACCTC 
GGCAACAAGC GGCGTGACGA CAACACTCCA GACAAAGATA CTCCACAACG CCGCAGAGGT 
AAAGACTCCG GTGTGGTGGT GACAGATGAT GCTCGTGAGC AGCTGTTGGA GGCTTCAGCT 
GCGACTAAGA AGGCCTTTGG TTCATATCGT CGAGATGCTG ATCCCGAGGA GACGTTTGGC 
ACGGCTGATG GAGATAAAGG AGGTGGAGAT AAAGGCACAG AGCAGGGCGA GATGAGCCCT 
GTCATCCTCA TAATGCAGCC AATCCTGCGC TTCCTGCAGC TGCTGTGCGA AAACCACAAT 
CGAGACCTGC AGAATTTTCT GCGCTGTCAG AACAATAAAA ACAACTATAA TCTGGTGTGT 
GAGACGCTGC AGTTCCTGGA CTGTATCTGT GGCAGCACAA CAGGAGGCCT GGGGCTGTTA 
GGACTCTACA TCAACCAGCA TAACGTAGCG CTCATTAATC AGACTGTAGA GAGCCTCACT 
GAATACTGTC AAGGTCCCTG CCATGACAAT CAGAATTGCA TTGCCACCCA TGAGTCTAAT 
GGCATCGACA TCATTATTGC ACTGATTCTG AATGATATTA ATCCACTGGG CAGGAAGAGA 
ATGGATCTGG TTCTAGAACT AAAGAATAAT GCATCTAAGC TGCTGCTGGC CATCATGGAG 
AGCCGTCATG ACAGTGAGAA TGCAGAAAGA ATTCTGTACA ACATGAGACC AAAAGAACTA 
GTGGAGGTGA TGAAAATGGC CTACCAGCAG GGGGAGGCAG AGTTTGAGGA TGAGGAACAG 
GAGAATGGAG AGGATCATGC AGCATCGCCT CGCAATGTTG GACACAACAT CTATATTCTT 
GCACATCAGC TGTCGCGTCA CAATAAGGAA TTGCAAATAC TACTGAAACC AGGTGGAGAG 
GACCAGGCCC TGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332899.1
CDS	148..6645
Translation	MVDKLSSFLHIGDVCSLYAEGSTSGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGTSTTDTVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDAAGNEGSWFYIQPFYKLRSIGDNVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCTTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDEHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEVNPDYEEECLESRSSMDSEHEVIRARARNPQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSPLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAAKLEKGTITQNERRSVTKLLEDLVYFVVDIPSSAQDVLEITVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRYSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDPANADILIETKLVLSRFEVAGTVLGEGAEEEEEDEEEVWLFWKDSGGEVKSKSIRELAQDAKDGHTEDQEVINYYRYQLNLFARMCLDRQYLAINKISAQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSQISIDDYDNDGTSSDEVKERFSQTMEFVENYLRDVVCQSFPFSDKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHISTPFHIKLDREPGKGSNVMRSIHGVGELMTQVVLRGGGFLPASSHNPPDRDEVKAQSEPQKQDILVMDTKLKIIEILQFILNVRLDYRISCLLCIFKTEFDESNSQSEPSLSPESPASVQGALDFEHIEEQAEGIFGGSSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALHLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSDSGLDAGEVTPEAHHKGVINSSRSNKPKVESTSSSNYRVVKEILLRLSKLCVLEGISGKKNKKQQQRLLRNMGAHSVVLELLQIPYEKGEDVQMQEIMTLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAVTMQHIFMNNFQLCSEINERVVQHFVHCIETHGRSVHYLKFLQTIVKAENKFIKKCQDIVMAELVTAGEDVLVFYNDRASFQSLVQMMRLERERLDESSALRYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKMAYVNFLNHCYVDTEVEMKEIYTSNHMWKLFDDFLVDVCRVCNNTSDRKHADTVLERYVTETIMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQGVFRVYHCNWLLPGQKGNVEACIKVLSDVAKGRAIAIPVDLDCQVNNLFMKSNNIVQKTALSWRLSVRNATRRDSVLTTSRDYRNIIERLQDIVSALEERLRPLVQAELSVLVDVLHRPELLFPEHTEAHRKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKLLGYDDEMDVTEVVDISLPPKQQEDRQRGEALRQLLVNRYYGFRSGGRRESLTSFGNSTLTPVGPIKSQPGGLSRAEMSLMEVQCHLDREGASDLVIDLIMNTTSDRVFHESILLAIALLEGGNTIIQHSFFKRLTEDKNSEKFFRVFYDRMKVAQVEIKATVTVNTSDLGNKRRDDNTPDKDTPQRRRGKDSGVVVTDDAREQLLEASAATKKAFGSYRRDADPEETFGTADGDKGGGDKGTEQGEMSPVILIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINQHNVALINQTVESLTEYCQGPCHDNQNCIATHESNGIDIIIALILNDINPLGRKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVMKMAYQQGEAEFEDEEQENGEDHAASPRNVGHNIYILAHQLSRHNKELQILLKPGGEDQALE

Target ORF information:

RefSeq Version	XM_021477224.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021477224.1

ORF Insert Sequence:

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GGTAAATCCA 
GACTATGAAG AAGAATGCCT GGAGTCCCGC TCCTCTATGG ATTCGGAGCA TGAAGTGATT 
CGGGCTCGTG CACGTAACCC TCAGGATAAG GTGATGTACA CACTGGTGTC TGTTCCTGAT 
GGAAATGACA TATCCTCCAT CTTTGAGCTT GACCCTACAA CTTTGAGGGG CGGAGACTCA 
CTTGTGCCAA GAAACTCGTA TGTGAGGCTC AGGCATCTCT GCACCAACAC GTGGGTCCAC 
AGCACCAACC AGCCAATAGA TAAAGAGGAA GAGAAACCGG TCATGCTCAG AATAGGAACG 
TCTCCTCTGA AGGAAGATAA AGAGGCGTTT GCTATAGTAC CAGTCTCTCC AGCTGAGGTT 
CGAGATCTGG ATTTTGCTAA TGATGCTAGT AAAGTGTTGG CTTCCATTGC TGCCAAACTG 
GAGAAGGGCA CCATTACCCA GAATGAGAGG AGGTCAGTGA CGAAGCTGTT GGAGGATCTG 
GTGTATTTTG TGGTGGACAT ACCCAGCAGT GCTCAAGATG TGCTGGAGAT CACAGTCAAC 
AAACCCAACA GAGAGCGACA GAAACTCATG AGAGAGCAGA ACATTCTTAA ACAGATCTTC 
AAGCTGCTGC AGGCGCCATT TACAGACAGC GGGGACGGCC CTATGTTGCG ATTAGAAGAG 
CTCGCAGACC AGCGTCATGC ACCCTTTCGA CACATTTGCA GACTCTGTTA CAGAGTCCTA 
CGTTACTCAC AACAGGACTA TCGCAAAAAT CAGGAGTACA TAGCAAAGCA GTTCCGCTTC 
ATGCAGAAGC AGATAGGATA TGATGTGCTG GCTGAAGACA CAATTACAGC CCTTCTGCAC 
AACAACAGGA AGCTCCTAGA GAAACACATC ACAGCAGCTG AAATAGATAC CTTTGTCAGT 
CTGGTCCGCA AGAACAGAGA GCCCAGATTT CTGGATTACT TGTCAGATCT GTGTGTGTCG 
ATGAATAAAT CCATCCCGGT CACTCAAGAG CTCATCTGTA ATGCTGTGCT GGACCCAGCC 
AATGCAGACA TTCTCATCGA AACCAAGCTT GTGTTGTCAC GGTTTGAGGT GGCAGGAACG 
GTGTTGGGTG AAGGTGCGGA GGAGGAAGAG GAGGATGAAG AGGAGGTCTG GCTGTTCTGG 
AAGGACAGTG GAGGAGAGGT GAAGAGTAAA AGCATACGAG AGCTTGCACA GGACGCAAAA 
GATGGACATA CAGAGGACCA GGAAGTCATC AATTATTACA GATATCAGCT GAATTTGTTT 
GCTCGGATGT GTCTGGATCG ACAGTATCTT GCGATTAACA AGATATCAGC ACAGCTGGAT 
GTGGATCTGA TTCTGCGCTG CATGTCAGAT GAAGATTTGC CCTTTGACCT CAGAGCATCG 
TTCTGCCGCA TGATGCTGCA CATGCATGTA GACCGTGACC CTCAGGAGCA GGTCACCCCG 
GTCAAATATG CTCGACTGTG GTCTGAAATC CCATCACAAA TTTCCATTGA TGATTATGAT 
AATGATGGGA CGTCAAGTGA TGAAGTGAAA GAGCGCTTCT CTCAGACGAT GGAGTTTGTG 
GAGAATTACC TGAGGGATGT TGTCTGCCAG AGTTTCCCGT TCTCTGATAA GGAGAAAAAC 
AAACTCACTT TTGAGGTGGT GAACCTGGCA AGAAACCTGA TCTATTTTGG TTTCTATAAC 
TTCAGTGATC TGCTAAGGTT AACAAAAATC CTATTGGCAA TCCTGGACTG TGTCCACATA 
AGCACTCCCT TCCACATCAA GCTGGATAGA GAACCCGGTA AAGGAAGTAA CGTGATGCGC 
TCCATCCATG GGGTGGGAGA GCTGATGACA CAGGTGGTGT TGAGGGGTGG GGGATTTCTG 
CCAGCCTCCA GCCACAATCC TCCTGACAGA GACGAAGTGA AAGCACAAAG TGAACCTCAG 
AAACAGGACA TACTGGTGAT GGACACTAAA CTCAAGATCA TCGAGATCCT GCAGTTTATT 
CTGAATGTGC GGTTGGACTA CAGGATCTCC TGCCTCCTCT GTATCTTTAA GACAGAGTTT 
GATGAAAGTA ACTCTCAAAG TGAACCATCT TTAAGCCCAG AATCTCCTGC CAGCGTTCAA 
GGTGCGCTAG ACTTTGAGCA CATTGAAGAG CAGGCGGAGG GTATTTTTGG AGGAAGTAGT 
GAAGAGAACA CCCCGCTGGA TCTTGATGAT CATGGTGGAC GGACATTTCT GAGGGTCCTG 
CTTCACCTGA CCATGCATGA CTATCCTCCG CTGGTATCTC GAGCTCTTCA CCTGCTCTTC 
AGACACTTTA GCCAACGCCA GGAGGTCCTA CAGGCCTTCA AACAGGTTCA GCTCCTGGTG 
ACCAGTCAGG ATGTGGAAAA CTATAAACAG ATCAAGTCAG ATTTGGACCA GCTGAGGTCA 
ATAGTAGAGA AATCAGAGTT GTGGGTGTAT AAGAGACAAG GCTCAGACTC AGGACTGGAT 
GCTGGAGAAG TCACCCCTGA AGCCCACCAC AAGGGTGTCA TCAACTCGAG TAGATCAAAC 
AAACCCAAAG TGGAAAGTAC CAGCAGCTCG AACTACAGAG TCGTGAAAGA GATCCTGCTG 
AGGCTGAGCA AACTGTGTGT TTTGGAGGGC ATCTCTGGCA AGAAGAATAA GAAACAGCAG 
CAGCGGCTCC TCAGGAACAT GGGAGCTCAC AGTGTGGTCC TCGAACTGTT GCAAATCCCT 
TATGAGAAGG GGGAGGATGT CCAGATGCAG GAGATCATGA CCTTAGCACA TCAGTTCCTT 
CAAAACTTCT GTGCTGGAAA TCAGCAGAAC CAGGCACTTC TACACAAACA CATCAACCTG 
TTCCTCAACC CTGGGATCCT GGAGGCGGTG ACCATGCAGC ATATCTTCAT GAATAATTTC 
CAGTTGTGTA GTGAGATTAA TGAGCGGGTC GTGCAGCATT TTGTGCACTG CATCGAGACA 
CACGGCCGCA GCGTGCACTA CCTCAAGTTC CTGCAGACCA TTGTCAAGGC AGAGAACAAG 
TTCATCAAGA AATGCCAAGA CATCGTCATG GCTGAGCTGG TGACTGCAGG TGAGGATGTG 
CTGGTGTTCT ATAATGACCG GGCGTCTTTT CAGTCATTGG TTCAGATGAT GCGTCTTGAG 
CGGGAACGAC TTGATGAGAG CAGTGCACTC AGGTATCACA TTCACCTGGT GGAGTTGCTG 
GCTGTGTGCA CTGAAGGCAA GAACGTCTAC ACTGAGATCA AATGCAACTC GCTGCTCCCT 
CTCGATGATA TTGTACGTGT GGTCACACAT GAGGACTGCA TCCCTGAGGT GAAGATGGCC 
TACGTGAACT TCCTGAATCA CTGTTATGTT GATACAGAGG TTGAGATGAA GGAGATCTAC 
ACCAGCAATC ACATGTGGAA ACTTTTTGAT GATTTTCTGG TGGACGTCTG CAGAGTGTGC 
AATAACACAA GTGACCGTAA ACACGCTGAC ACAGTTCTGG AGAGATACGT TACAGAGACC 
ATAATGAGCA TCGTCACCAC CTTTTTCAGC TCACCATTCT CAGACCAGAG CACAAGTTTG 
CAGACCCGGC AGCCGGTGTT TGTGCAGCTG CTGCAGGGAG TGTTTCGTGT ATATCACTGC 
AACTGGCTTC TCCCAGGTCA GAAGGGGAAT GTGGAAGCCT GCATCAAAGT GCTGAGTGAC 
GTGGCCAAAG GCAGAGCCAT AGCCATTCCT GTGGATCTGG ACTGTCAGGT GAATAATCTG 
TTCATGAAGT CCAACAACAT TGTGCAGAAA ACAGCCCTCA GCTGGAGACT CTCTGTCCGT 
AACGCGACTC GGAGAGACTC TGTTCTGACC ACCTCTCGAG ACTATCGCAA CATCATTGAA 
AGACTACAGG ATATAGTGTC TGCATTGGAG GAGCGGTTGA GGCCTCTGGT TCAGGCTGAG 
TTGTCAGTGT TGGTGGATGT TCTCCATCGC CCAGAGCTGC TGTTTCCAGA GCACACTGAA 
GCCCACCGCA AGTGTGAGAG TGGAGGCTTC ATCTGCAAGT TGATAAAACA CACTAAGCAG 
CTTCTAGAAG AAAACGAAGA GAGGCTTTGC ATCAAAGTCC TTCAGACACT CCGAGAGATG 
ATGACCAAAG ACAGAGGATA TGGAGAGAAG CTTCTGGGAT ATGATGATGA GATGGATGTC 
ACGGAGGTGG TGGATATCAG TCTGCCACCC AAACAGCAGG AGGATCGGCA GAGGGGGGAG 
GCCCTTCGGC AGCTGCTCGT CAATCGTTAC TATGGCTTCA GGAGTGGTGG GCGGAGAGAA 
AGTCTGACCT CGTTTGGTAA CTCCACCCTA ACTCCTGTCG GGCCAATCAA GAGCCAGCCA 
GGGGGGTTGA GCAGGGCTGA GATGTCATTA ATGGAGGTGC AGTGTCATCT AGACCGTGAA 
GGGGCATCGG ATCTTGTCAT TGATCTCATC ATGAACACTA CCAGCGACCG CGTCTTCCAT 
GAAAGCATTC TGTTGGCCAT CGCACTGTTG GAGGGCGGAA ACACCATCAT ACAGCATTCC 
TTCTTCAAAC GTTTAACAGA AGACAAAAAT TCAGAGAAGT TTTTCAGGGT GTTTTATGAC 
AGAATGAAAG TGGCTCAGGT GGAGATCAAA GCCACGGTTA CTGTCAACAC TAGTGACCTC 
GGCAACAAGC GGCGTGACGA CAACACTCCA GACAAAGATA CTCCACAACG CCGCAGAGGT 
AAAGACTCCG GTGTGGTGGT GACAGATGAT GCTCGTGAGC AGCTGTTGGA GGCTTCAGCT 
GCGACTAAGA AGGCCTTTGG TTCATATCGT CGAGATGCTG ATCCCGAGGA GACGTTTGGC 
ACGGCTGATG GAGATAAAGG AGGTGGAGAT AAAGGCACAG AGCAGGGCGA GATGAGCCCT 
GTCATCCTCA TAATGCAGCC AATCCTGCGC TTCCTGCAGC TGCTGTGCGA AAACCACAAT 
CGAGACCTGC AGAATTTTCT GCGCTGTCAG AACAATAAAA ACAACTATAA TCTGGTGTGT 
GAGACGCTGC AGTTCCTGGA CTGTATCTGT GGCAGCACAA CAGGAGGCCT GGGGCTGTTA 
GGACTCTACA TCAACCAGCA TAACGTAGCG CTCATTAATC AGACTGTAGA GAGCCTCACT 
GAATACTGTC AAGGTCCCTG CCATGACAAT CAGAATTGCA TTGCCACCCA TGAGTCTAAT 
GGCATCGACA TCATTATTGC ACTGATTCTG AATGATATTA ATCCACTGGG CAGGAAGAGA 
ATGGATCTGG TTCTAGAACT AAAGAATAAT GCATCTAAGC TGCTGCTGGC CATCATGGAG 
AGCCGTCATG ACAGTGAGAA TGCAGAAAGA ATTCTGTACA ACATGAGACC AAAAGAACTA 
GTGGAGGTGA TGAAAATGGC CTACCAGCAG GGGGAGGCAG AGTTTGAGGA TGAGGAACAG 
GAGAATGGAG AGGATCATGC AGCATCGCCT CGCAATGTTG GACACAACAT CTATATTCTT 
GCACATCAGC TGTCGCGTCA CAATAAGGAA TTGCAAATAC TACTGAAACC AGGTGGAGAG 
GACCAGGCCC TGGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itpr1a ( XM_021474619.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330294.1 Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X15, mRNA.

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CloneID	ODa146878
Clone ID Related Accession (Same CDS sequence)	XM_021474619.1
Accession Version	XM_021474619.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395382.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACAT TACAAGGTGC TGGAGTTTTT GCCATGGTGG ACAAATTGTC TAGTTTCCTG 
CACATTGGGG ACGTTTGTTC CCTTTATGCT GAAGGCTCTA CTAGTGGCTT CATCAGCACT 
CTGGGGTTGG TTGATGACCG ATGTGTTGTT CAACCAGATG CCGGAGACCT GAATAACCCA 
CCTAAAAAGT TTCGGGACTG TCTGTTCAGA CTGTGTCCCA TGAACAGATA TTCGGCACAG 
AAACAGTTCT GGAAAGCTGC CAAACCTGGA GGAACTAGCA CCACAGATAC TGTCCTGCTT 
AACAAACTCC ATCATGCTGC TGATTTAGAG AAGAAACAAA ATGATTCTGA GAACAAAAAG 
CTGCTTGGAA CCGTCATTCA GTATGGCAAT GTCATCCAGC TTCTCCATCT GAAGAGTAAT 
AAATATCTGA CGGTGAATAA GCGTTTGCCG GCTCTTCTTG AGAAGAATGC CATGCGTGTG 
ACTCTGGACG CTGCAGGGAA TGAAGGCTCG TGGTTTTACA TCCAGCCCTT CTATAAGCTG 
CGCTCTATAG GGGATAATGT GGTCATTGGA GATAAGGTCG TGTTAAACCC TGTCAATGCC 
GGACAGCCGC TGCACGCCAG CAGCCATCAG CTTGTGGATA ATCCAGGCTG CAATGAAGTG 
AATTCAGTGA ACTGCACCAC CAGTTGGAAA ATCGTTCTCT TCATGAAATG GAGCGACAAC 
CAGGAGATTG TCTTGAAAGG AGGTGACGTG GTGAGGCTGT TTCATGCAGA GCAGGAGAAG 
TTTCTGACAT GTGATGAGCA TCGGAAAAAG CAGTATGTGT TTCTGCGTAC CACCGGACGC 
CAGTCTGCAA CCTCCGCCAC TAGTAGCAAA GCACTCTGGG AGGTAGAGGT GGTTCAGCAT 
GATCCCTGCC GAGGCGGAGC CGGCTACTGG AACAGCCTCT TTAGGTTTAA ACACTTAGCC 
ACCGGCCATT ACCTTGCTGC AGAGGTAAAT CCAGACTATG AAGAAGAATG CCTGGAGTCC 
CGCTCCTCTA TGGATTCGGA GCATGAAGTG ATTCGGGCTC GTGCACGTAA CCCTCAGGAT 
AAGGTGATGT ACACACTGGT GTCTGTTCCT GATGGAAATG ACATATCCTC CATCTTTGAG 
CTTGACCCTA CAACTTTGAG GGGCGGAGAC TCACTTGTGC CAAGAAACTC GTATGTGAGG 
CTCAGGCATC TCTGCACCAA CACGTGGGTC CACAGCACCA ACCAGCCAAT AGATAAAGAG 
GAAGAGAAAC CGGTCATGCT CAGAATAGGA ACGTCTCCTC TGAAGGAAGA TAAAGAGGCG 
TTTGCTATAG TACCAGTCTC TCCAGCTGAG GTTCGAGATC TGGATTTTGC TAATGATGCT 
AGTAAAGTGT TGGCCTCCAT TGCTGCCAAA CTGGAGAAGG GCACCATCAC CCAGAATGAG 
AGGAGGTCAG TGACGAAGCT GTTGGAGGAT CTGGTGTATT TTGTGGTGGA CATACCCAGC 
AGTGCTCAAG ATGTGCTGGA GATCACAGTC AACAAACCCA ACAGAGAGCG ACAGAAACTC 
ATGAGAGAGC AGAACATTCT TAAACAGATC TTCAAGCTGC TGCAGGCGCC ATTTACAGAC 
AGCGGGGACG GCCCTATGTT GCGATTAGAA GAGCTCGCAG ACCAGCGTCA TGCACCCTTT 
CGACACATTT GCAGACTCTG TTACAGAGTC CTACGTTACT CACAACAGGA CTATCGCAAA 
AATCAGGAGT ACATAGCAAA GCAGTTCCGC TTCATGCAGA AGCAGATAGG ATATGATGTG 
CTGGCTGAAG ACACAATTAC AGCCCTTCTG CACAACAACA GGAAGCTCCT AGAGAAACAC 
ATCACAGCAG CTGAAATAGA TACCTTTGTC AGTCTGGTCC GCAAGAACAG AGAGCCCAGA 
TTTCTGGATT ACTTGTCAGA TCTGTGTGTG TCGATGAATA AATCCATCCC GGTCACTCAA 
GAGCTCATCT GTAATGCTGT GCTGGACCCA GCCAATGCAG ACATTCTCAT CGAAACCAAG 
CTTGTGTTGT CACGATTTGA GGTGGCAGGA ACGGTGTTGG GTGAAGGTGC GGAGGAGGAA 
GAGGAGGATG AAGAGGAGGT CTGGCTGTTC TGGAAGGACA GTGGAGGAGA GGTGAAGAGT 
AAAAGCATAC GAGAGCTTGC ACAGGACGCA AAAGATGGAC ATACAGAGGA CCAGGAAGTC 
ATCAATTATT ACAGATATCA GCTGAATTTG TTTGCTCGGA TGTGTCTGGA TCGACAGTAT 
CTTGCGATTA ACAAGATATC AGCACAGCTG GATGTGGATC TGATTCTGCG CTGCATGTCA 
GATGAAGATT TGCCCTTTGA CCTCAGAGCA TCGTTCTGCC GCATGATGCT GCACATGCAT 
GTAGACCGTG ACCCTCAGGA GCAGGTCACC CCGGTCAAAT ATGCTCGACT GTGGTCTGAA 
ATCCCATCAC AAATTTCCAT TGATGATTAT GATAATGATG GGACGTCACG TGATGAAGTG 
AAAGAGCGCT TCTCTCAGAC GATGGAGTTT GTGGAGAATT ACCTGAGGGA TGTTGTCTGC 
CAGAGTTTCC CGTTCTCTGA TAAGGAGAAA AACAAACTCA CTTTTGAGGT GGTGAACCTG 
GCAAGAAACC TGATCTATTT TGGTTTCTAT AACTTCAGTG ATCTGCTAAG GTTAACAAAA 
ATCCTATTGG CAATCCTGGA CTGTGTCCAC ATAAGCACTC CCTTCCACAT CAAGCTGGAT 
AGAGAACCTG GTAAAGGAAG TAACGTGATG CGCTCCATCC ATGGGGTGGG AGAACTGATG 
ACACAGGTAG TGTTGAGGGG TGGGGGATTT CTGCCAGCCT CCAGCCACAA TCCTCCTGAC 
AGAGACGAAG TGAAAGCACA AAGTGAACCT CAGAAACAAG ACATACTGGT GATGGACACT 
AAACTCAAGA TCATCGAGAT CCTGCAGTTT ATTCTGAATG TGCGGTTGGA CTACAGGATC 
TCCTGCCTCC TCTGTATCTT TAAGACAGAG TTTGATGAAA GTAACTCTCA AAGTGAACCA 
TCTTTAAGCC CAGAATCTCC TGCCAGCGTT CAAGGTGCGC TAGACTTTGA GCACATTGAA 
GAGCAGGCGG AGGGTATTTT TGGAGGAAGT AGTGAAGAGA ACACCCCGCT GGATCTTGAT 
GATCATGGTG GACGGACATT TCTGAGGGTC CTGCTTCACC TGACCATGCA TGACTATCCT 
CCGCTGGTAT CTCGAGCTCT TCACCTGCTC TTCAGACACT TTAGCCAACG CCAGGAGGTC 
CTACAGGCCT TCAAACAGGT TCAGCTCCTG GTGACCAGTC AGGATGTGGA AAACTATAAA 
CAGATCAAGT CAGATTTGGA CCAGCTGAGG TCAATAGTAG AGAAATCAGA GTTGTGGGTG 
TATAAGAGAC AAGGCTCAGA CTCAGGACTG GATGCTGGAG AAGTCACCCC TGAAGCCCAC 
CACAAGGGTG TCATCAACTC GAGTAGATCA AACAAACCCA AAGTGGAAAG TACCAGCAGC 
TCGAACTACA GAGTCGTGAA AGAGATCCTG CTGAGGCTGA GCAAACTGTG TGTTTTGGAG 
GGCATCTCTG GCAAGAAGAA TAAGAAACAG CAGCAGCGGC TCCTCAGGAA CATGGGAGCT 
CACAGTGTGG TCCTCGAACT GTTGCAAATC CCTTATGAGA AGGGGGAGGA TGTCCAGATG 
CAGGAGATCA TGACCTTAGC ACATCAGTTC CTTCAAAACT TCTGTGCTGG AAATCAGCAG 
AACCAGGCAC TTCTACACAA ACACATCAAC CTGTTCCTCA ACCCTGGGAT CCTGGAGGCG 
GTGACCATGC AGCATATCTT CATGAATAAT TTCCAGTTGT GTAGTGAGAT TAATGAGCGG 
GTCGTGCAGC ATTTTGTGCA CTGCATCGAG ACACACGGCC GCAGCGTGCA CTACCTCAAG 
TTCCTGCAGA CCATTGTCAA GGCAGAGAAC AAGTTCATCA AGAAATGCCA AGACATCGTC 
ATGGCTGAGC TGGTGACTGC AGGTGAGGAT GTGCTGGTGT TCTATAATGA CCGGGCGTCT 
TTTCAGTCAT TGGTTCAGAT GATGCGTCTT GAGCGGGAAC GACTTGATGA GAGCAGTGCA 
CTCAGGTATC ACATTCACCT GGTGGAGTTG CTGGCTGTGT GCACTGAAGG CAAGAACGTC 
TACACTGAGA TCAAATGCAA CTCGCTGCTC CCTCTCGATG ATATTGTACG TGTGGTCACA 
CATGAGGACT GCATCCCTGA GGTGAAGATG GCCTACGTGA ACTTCCTGAA TCACTGTTAT 
GTTGATACAG AGGTTGAGAT GAAGGAGATC TACACCAGCA ATCACATGTG GAAACTTTTT 
GATGATTTTC TGGTGGACGT CTGCAGAGTG TGCAATAACA CAAGTGACCG TAAACACGCT 
GACACAGTTC TGGAGAGATA CGTTACAGAG ACCATAATGA GCATCGTCAC CACCTTTTTC 
AGCTCACCAT TCTCAGACCA GAGCACAAGT TTGCAGACCC GGCAGCCGGT GTTTGTGCAG 
CTGCTGCAGG GAGTGTTTCG TGTATATCAC TGCAACTGGC TTCTCCCAGG TCAGAAGGGG 
AATGTGGAAG CCTGCATCAA AGTGCTGAGT GACGTGGCCA AAGGCAGAGC CATAGCCATT 
CCTGTGGATC TGGACTGTCA GGTGAATAAT CTGTTCATGA AGTCCAACAA CATTGTGCAG 
AAAACAGCCC TCAGCTGGAG ACTCTCTGTC CGTAACGCGA CTCGGAGAGA CTCTGTTCTG 
ACCACCTCTC GAGACTATCG CAACATCATT GAAAGACTAC AGGATATAGT GTCTGCATTG 
GAGGAGCGGT TGAGGCCTCT GGTTCAGGCT GAGTTGTCAG TGTTGGTGGA TGTTCTCCAT 
CGCCCAGAGC TGCTGTTTCC AGAGCACACT GAAGCCCACC GCAAGTGTGA GAGTGGAGGC 
TTCATCTGCA AGTTGATAAA ACACACTAAG CAGCTTCTAG AAGAAAACGA AGAGAGGCTT 
TGCATCAAAG TCCTTCAGAC ACTCCGAGAG ATGATGACCA AAGACAGAGG ATATGGAGAG 
AAGCTTCTGG GATATGATGA TGAGATGGAT GTCACGGAGG TGGTGGATAT CAGTCTGCCA 
CCCAAACAGC AGGAGGATCG GCAGAGGGGG GAGGCCCTTC GGCAGCTGCT CGTCAATCGT 
TACTATGGCT TCAGGAGTGG TGGGCGGAGA GAAAGTCTGA CCTCGTTTGG TAACTCCACC 
CTAACTCCTG TCGGGCCAAT CAAGAGCCAG CCAGGGGGGT TGAGCAGGGC TGAGATGTCA 
TTAATGGAGG TGCAGTGTCA TCTAGACCGT GAAGGGGCAT CGGATCTTGT CATTGATCTC 
ATCATGAACA CTACCAGCGA CCGCGTCTTC CATGAAAGCA TTCTGTTGGC CATCGCACTG 
TTGGAGGGCG GAAACACCAT CATACAGCAT TCCTTCTTCA AACGTTTAAC AGAAGACAAA 
AATTCAGAGA AGTTTTTCAG GGTGTTTTAT GACAGAATGA AAGTGGCTCA GGTGGAGATC 
AAAGCCACGG TTACTGTCAA CACTAGTGAC CTCGGCAACA AGCGGCGTGA CGACAACACT 
CCAGACAAAG ATACTCCACA ACGCCGCAGA GGTAAAGACT CCGGTGTGGT GGTGACAGAT 
GATGCTCGTG AGCAGCTGTT GGAGGCTTCA GCTGCGACTA AGAAGGCCTT TGGTTCATAT 
CGTCGAGATG CTGATCCCGA GGAGACGTTT GGCACGGCTG ATGGAGATAA AGGAGGTGGA 
GATAAAGGCA CAGAGCAGGG CGAGATGAGC CCTGTCATCC TCATAATGCA GCCAATCCTG 
CGCTTCCTGC AGCTGCTGTG CGAAAACCAC AATCGAGACC TGCAGAATTT TCTGCGCTGT 
CAGAACAATA AAAACAACTA TAATCTGGTG TGTGAGACGC TGCAGTTCCT GGACTGTATC 
TGTGGCAGCA CAACAGGAGG CCTGGGGCTG TTAGGACTCT ACATCAACCA GCATAACGTA 
GCGCTCATTA ATCAGACTGT AGAGAGCCTC ACTGAATACT GTCAAGGTCC CTGCCATGAC 
AATCAGAATT GCATTGCCAC CCATGAGTCT AATGGCATCG ACATCATTAT TGCACTGATT 
CTGAATGATA TTAATCCACT GGGCAGGAAG AGAATGGATC TGGTTCTAGA ACTAAAGAAT 
AATGCATCTA AGCTGCTGCT GGCCATCATG GAGAGCCGTC ATGACAGTGA GAATGCAGAA 
AGAATTCTGT ACAACATGAG ACCAAAAGAA CTAGTGGAGG TGATGAAAAT GGCCTACCAG 
CAGGGGGAGG CAGAGTTTGA GGATGAGGAA CAGGAGAATG GAGAGGATCA TGCAGCATCG 
CCTCGCAATG TTGGACACAA CATCTATATT CTTGCACATC AGCTGTCGCG TCACAATAAG 
GAATTGCAAA TACTACTGAA ACCAGGTGGA GAGGACCAGG CCCTGGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330294.1
CDS	270..6800
Translation	MMTLQGAGVFAMVDKLSSFLHIGDVCSLYAEGSTSGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGTSTTDTVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDAAGNEGSWFYIQPFYKLRSIGDNVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCTTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDEHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEVNPDYEEECLESRSSMDSEHEVIRARARNPQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSPLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAAKLEKGTITQNERRSVTKLLEDLVYFVVDIPSSAQDVLEITVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRYSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDPANADILIETKLVLSRFEVAGTVLGEGAEEEEEDEEEVWLFWKDSGGEVKSKSIRELAQDAKDGHTEDQEVINYYRYQLNLFARMCLDRQYLAINKISAQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSQISIDDYDNDGTSRDEVKERFSQTMEFVENYLRDVVCQSFPFSDKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHISTPFHIKLDREPGKGSNVMRSIHGVGELMTQVVLRGGGFLPASSHNPPDRDEVKAQSEPQKQDILVMDTKLKIIEILQFILNVRLDYRISCLLCIFKTEFDESNSQSEPSLSPESPASVQGALDFEHIEEQAEGIFGGSSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALHLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSDSGLDAGEVTPEAHHKGVINSSRSNKPKVESTSSSNYRVVKEILLRLSKLCVLEGISGKKNKKQQQRLLRNMGAHSVVLELLQIPYEKGEDVQMQEIMTLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAVTMQHIFMNNFQLCSEINERVVQHFVHCIETHGRSVHYLKFLQTIVKAENKFIKKCQDIVMAELVTAGEDVLVFYNDRASFQSLVQMMRLERERLDESSALRYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKMAYVNFLNHCYVDTEVEMKEIYTSNHMWKLFDDFLVDVCRVCNNTSDRKHADTVLERYVTETIMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQGVFRVYHCNWLLPGQKGNVEACIKVLSDVAKGRAIAIPVDLDCQVNNLFMKSNNIVQKTALSWRLSVRNATRRDSVLTTSRDYRNIIERLQDIVSALEERLRPLVQAELSVLVDVLHRPELLFPEHTEAHRKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKLLGYDDEMDVTEVVDISLPPKQQEDRQRGEALRQLLVNRYYGFRSGGRRESLTSFGNSTLTPVGPIKSQPGGLSRAEMSLMEVQCHLDREGASDLVIDLIMNTTSDRVFHESILLAIALLEGGNTIIQHSFFKRLTEDKNSEKFFRVFYDRMKVAQVEIKATVTVNTSDLGNKRRDDNTPDKDTPQRRRGKDSGVVVTDDAREQLLEASAATKKAFGSYRRDADPEETFGTADGDKGGGDKGTEQGEMSPVILIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINQHNVALINQTVESLTEYCQGPCHDNQNCIATHESNGIDIIIALILNDINPLGRKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVMKMAYQQGEAEFEDEEQENGEDHAASPRNVGHNIYILAHQLSRHNKELQILLKPGGEDQALE

Target ORF information:

RefSeq Version	XM_021474619.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474619.1

ORF Insert Sequence:

ATGATGACAT TACAAGGTGC TGGAGTTTTT GCCATGGTGG ACAAATTGTC TAGTTTCCTG 
CACATTGGGG ACGTTTGTTC CCTTTATGCT GAAGGCTCTA CTAGTGGCTT CATCAGCACT 
CTGGGGTTGG TTGATGACCG ATGTGTTGTT CAACCAGATG CCGGAGACCT GAATAACCCA 
CCTAAAAAGT TTCGGGACTG TCTGTTCAGA CTGTGTCCCA TGAACAGATA TTCGGCACAG 
AAACAGTTCT GGAAAGCTGC CAAACCTGGA GGAACTAGCA CCACAGATAC TGTCCTGCTT 
AACAAACTCC ATCATGCTGC TGATTTAGAG AAGAAACAAA ATGATTCTGA GAACAAAAAG 
CTGCTTGGAA CCGTCATTCA GTATGGCAAT GTCATCCAGC TTCTCCATCT GAAGAGTAAT 
AAATATCTGA CGGTGAATAA GCGTTTGCCG GCTCTTCTTG AGAAGAATGC CATGCGTGTG 
ACTCTGGACG CTGCAGGGAA TGAAGGCTCG TGGTTTTACA TCCAGCCCTT CTATAAGCTG 
CGCTCTATAG GGGATAATGT GGTCATTGGA GATAAGGTCG TGTTAAACCC TGTCAATGCC 
GGACAGCCGC TGCACGCCAG CAGCCATCAG CTTGTGGATA ATCCAGGCTG CAATGAAGTG 
AATTCAGTGA ACTGCACCAC CAGTTGGAAA ATCGTTCTCT TCATGAAATG GAGCGACAAC 
CAGGAGATTG TCTTGAAAGG AGGTGACGTG GTGAGGCTGT TTCATGCAGA GCAGGAGAAG 
TTTCTGACAT GTGATGAGCA TCGGAAAAAG CAGTATGTGT TTCTGCGTAC CACCGGACGC 
CAGTCTGCAA CCTCCGCCAC TAGTAGCAAA GCACTCTGGG AGGTAGAGGT GGTTCAGCAT 
GATCCCTGCC GAGGCGGAGC CGGCTACTGG AACAGCCTCT TTAGGTTTAA ACACTTAGCC 
ACCGGCCATT ACCTTGCTGC AGAGGTAAAT CCAGACTATG AAGAAGAATG CCTGGAGTCC 
CGCTCCTCTA TGGATTCGGA GCATGAAGTG ATTCGGGCTC GTGCACGTAA CCCTCAGGAT 
AAGGTGATGT ACACACTGGT GTCTGTTCCT GATGGAAATG ACATATCCTC CATCTTTGAG 
CTTGACCCTA CAACTTTGAG GGGCGGAGAC TCACTTGTGC CAAGAAACTC GTATGTGAGG 
CTCAGGCATC TCTGCACCAA CACGTGGGTC CACAGCACCA ACCAGCCAAT AGATAAAGAG 
GAAGAGAAAC CGGTCATGCT CAGAATAGGA ACGTCTCCTC TGAAGGAAGA TAAAGAGGCG 
TTTGCTATAG TACCAGTCTC TCCAGCTGAG GTTCGAGATC TGGATTTTGC TAATGATGCT 
AGTAAAGTGT TGGCCTCCAT TGCTGCCAAA CTGGAGAAGG GCACCATCAC CCAGAATGAG 
AGGAGGTCAG TGACGAAGCT GTTGGAGGAT CTGGTGTATT TTGTGGTGGA CATACCCAGC 
AGTGCTCAAG ATGTGCTGGA GATCACAGTC AACAAACCCA ACAGAGAGCG ACAGAAACTC 
ATGAGAGAGC AGAACATTCT TAAACAGATC TTCAAGCTGC TGCAGGCGCC ATTTACAGAC 
AGCGGGGACG GCCCTATGTT GCGATTAGAA GAGCTCGCAG ACCAGCGTCA TGCACCCTTT 
CGACACATTT GCAGACTCTG TTACAGAGTC CTACGTTACT CACAACAGGA CTATCGCAAA 
AATCAGGAGT ACATAGCAAA GCAGTTCCGC TTCATGCAGA AGCAGATAGG ATATGATGTG 
CTGGCTGAAG ACACAATTAC AGCCCTTCTG CACAACAACA GGAAGCTCCT AGAGAAACAC 
ATCACAGCAG CTGAAATAGA TACCTTTGTC AGTCTGGTCC GCAAGAACAG AGAGCCCAGA 
TTTCTGGATT ACTTGTCAGA TCTGTGTGTG TCGATGAATA AATCCATCCC GGTCACTCAA 
GAGCTCATCT GTAATGCTGT GCTGGACCCA GCCAATGCAG ACATTCTCAT CGAAACCAAG 
CTTGTGTTGT CACGATTTGA GGTGGCAGGA ACGGTGTTGG GTGAAGGTGC GGAGGAGGAA 
GAGGAGGATG AAGAGGAGGT CTGGCTGTTC TGGAAGGACA GTGGAGGAGA GGTGAAGAGT 
AAAAGCATAC GAGAGCTTGC ACAGGACGCA AAAGATGGAC ATACAGAGGA CCAGGAAGTC 
ATCAATTATT ACAGATATCA GCTGAATTTG TTTGCTCGGA TGTGTCTGGA TCGACAGTAT 
CTTGCGATTA ACAAGATATC AGCACAGCTG GATGTGGATC TGATTCTGCG CTGCATGTCA 
GATGAAGATT TGCCCTTTGA CCTCAGAGCA TCGTTCTGCC GCATGATGCT GCACATGCAT 
GTAGACCGTG ACCCTCAGGA GCAGGTCACC CCGGTCAAAT ATGCTCGACT GTGGTCTGAA 
ATCCCATCAC AAATTTCCAT TGATGATTAT GATAATGATG GGACGTCACG TGATGAAGTG 
AAAGAGCGCT TCTCTCAGAC GATGGAGTTT GTGGAGAATT ACCTGAGGGA TGTTGTCTGC 
CAGAGTTTCC CGTTCTCTGA TAAGGAGAAA AACAAACTCA CTTTTGAGGT GGTGAACCTG 
GCAAGAAACC TGATCTATTT TGGTTTCTAT AACTTCAGTG ATCTGCTAAG GTTAACAAAA 
ATCCTATTGG CAATCCTGGA CTGTGTCCAC ATAAGCACTC CCTTCCACAT CAAGCTGGAT 
AGAGAACCTG GTAAAGGAAG TAACGTGATG CGCTCCATCC ATGGGGTGGG AGAACTGATG 
ACACAGGTAG TGTTGAGGGG TGGGGGATTT CTGCCAGCCT CCAGCCACAA TCCTCCTGAC 
AGAGACGAAG TGAAAGCACA AAGTGAACCT CAGAAACAAG ACATACTGGT GATGGACACT 
AAACTCAAGA TCATCGAGAT CCTGCAGTTT ATTCTGAATG TGCGGTTGGA CTACAGGATC 
TCCTGCCTCC TCTGTATCTT TAAGACAGAG TTTGATGAAA GTAACTCTCA AAGTGAACCA 
TCTTTAAGCC CAGAATCTCC TGCCAGCGTT CAAGGTGCGC TAGACTTTGA GCACATTGAA 
GAGCAGGCGG AGGGTATTTT TGGAGGAAGT AGTGAAGAGA ACACCCCGCT GGATCTTGAT 
GATCATGGTG GACGGACATT TCTGAGGGTC CTGCTTCACC TGACCATGCA TGACTATCCT 
CCGCTGGTAT CTCGAGCTCT TCACCTGCTC TTCAGACACT TTAGCCAACG CCAGGAGGTC 
CTACAGGCCT TCAAACAGGT TCAGCTCCTG GTGACCAGTC AGGATGTGGA AAACTATAAA 
CAGATCAAGT CAGATTTGGA CCAGCTGAGG TCAATAGTAG AGAAATCAGA GTTGTGGGTG 
TATAAGAGAC AAGGCTCAGA CTCAGGACTG GATGCTGGAG AAGTCACCCC TGAAGCCCAC 
CACAAGGGTG TCATCAACTC GAGTAGATCA AACAAACCCA AAGTGGAAAG TACCAGCAGC 
TCGAACTACA GAGTCGTGAA AGAGATCCTG CTGAGGCTGA GCAAACTGTG TGTTTTGGAG 
GGCATCTCTG GCAAGAAGAA TAAGAAACAG CAGCAGCGGC TCCTCAGGAA CATGGGAGCT 
CACAGTGTGG TCCTCGAACT GTTGCAAATC CCTTATGAGA AGGGGGAGGA TGTCCAGATG 
CAGGAGATCA TGACCTTAGC ACATCAGTTC CTTCAAAACT TCTGTGCTGG AAATCAGCAG 
AACCAGGCAC TTCTACACAA ACACATCAAC CTGTTCCTCA ACCCTGGGAT CCTGGAGGCG 
GTGACCATGC AGCATATCTT CATGAATAAT TTCCAGTTGT GTAGTGAGAT TAATGAGCGG 
GTCGTGCAGC ATTTTGTGCA CTGCATCGAG ACACACGGCC GCAGCGTGCA CTACCTCAAG 
TTCCTGCAGA CCATTGTCAA GGCAGAGAAC AAGTTCATCA AGAAATGCCA AGACATCGTC 
ATGGCTGAGC TGGTGACTGC AGGTGAGGAT GTGCTGGTGT TCTATAATGA CCGGGCGTCT 
TTTCAGTCAT TGGTTCAGAT GATGCGTCTT GAGCGGGAAC GACTTGATGA GAGCAGTGCA 
CTCAGGTATC ACATTCACCT GGTGGAGTTG CTGGCTGTGT GCACTGAAGG CAAGAACGTC 
TACACTGAGA TCAAATGCAA CTCGCTGCTC CCTCTCGATG ATATTGTACG TGTGGTCACA 
CATGAGGACT GCATCCCTGA GGTGAAGATG GCCTACGTGA ACTTCCTGAA TCACTGTTAT 
GTTGATACAG AGGTTGAGAT GAAGGAGATC TACACCAGCA ATCACATGTG GAAACTTTTT 
GATGATTTTC TGGTGGACGT CTGCAGAGTG TGCAATAACA CAAGTGACCG TAAACACGCT 
GACACAGTTC TGGAGAGATA CGTTACAGAG ACCATAATGA GCATCGTCAC CACCTTTTTC 
AGCTCACCAT TCTCAGACCA GAGCACAAGT TTGCAGACCC GGCAGCCGGT GTTTGTGCAG 
CTGCTGCAGG GAGTGTTTCG TGTATATCAC TGCAACTGGC TTCTCCCAGG TCAGAAGGGG 
AATGTGGAAG CCTGCATCAA AGTGCTGAGT GACGTGGCCA AAGGCAGAGC CATAGCCATT 
CCTGTGGATC TGGACTGTCA GGTGAATAAT CTGTTCATGA AGTCCAACAA CATTGTGCAG 
AAAACAGCCC TCAGCTGGAG ACTCTCTGTC CGTAACGCGA CTCGGAGAGA CTCTGTTCTG 
ACCACCTCTC GAGACTATCG CAACATCATT GAAAGACTAC AGGATATAGT GTCTGCATTG 
GAGGAGCGGT TGAGGCCTCT GGTTCAGGCT GAGTTGTCAG TGTTGGTGGA TGTTCTCCAT 
CGCCCAGAGC TGCTGTTTCC AGAGCACACT GAAGCCCACC GCAAGTGTGA GAGTGGAGGC 
TTCATCTGCA AGTTGATAAA ACACACTAAG CAGCTTCTAG AAGAAAACGA AGAGAGGCTT 
TGCATCAAAG TCCTTCAGAC ACTCCGAGAG ATGATGACCA AAGACAGAGG ATATGGAGAG 
AAGCTTCTGG GATATGATGA TGAGATGGAT GTCACGGAGG TGGTGGATAT CAGTCTGCCA 
CCCAAACAGC AGGAGGATCG GCAGAGGGGG GAGGCCCTTC GGCAGCTGCT CGTCAATCGT 
TACTATGGCT TCAGGAGTGG TGGGCGGAGA GAAAGTCTGA CCTCGTTTGG TAACTCCACC 
CTAACTCCTG TCGGGCCAAT CAAGAGCCAG CCAGGGGGGT TGAGCAGGGC TGAGATGTCA 
TTAATGGAGG TGCAGTGTCA TCTAGACCGT GAAGGGGCAT CGGATCTTGT CATTGATCTC 
ATCATGAACA CTACCAGCGA CCGCGTCTTC CATGAAAGCA TTCTGTTGGC CATCGCACTG 
TTGGAGGGCG GAAACACCAT CATACAGCAT TCCTTCTTCA AACGTTTAAC AGAAGACAAA 
AATTCAGAGA AGTTTTTCAG GGTGTTTTAT GACAGAATGA AAGTGGCTCA GGTGGAGATC 
AAAGCCACGG TTACTGTCAA CACTAGTGAC CTCGGCAACA AGCGGCGTGA CGACAACACT 
CCAGACAAAG ATACTCCACA ACGCCGCAGA GGTAAAGACT CCGGTGTGGT GGTGACAGAT 
GATGCTCGTG AGCAGCTGTT GGAGGCTTCA GCTGCGACTA AGAAGGCCTT TGGTTCATAT 
CGTCGAGATG CTGATCCCGA GGAGACGTTT GGCACGGCTG ATGGAGATAA AGGAGGTGGA 
GATAAAGGCA CAGAGCAGGG CGAGATGAGC CCTGTCATCC TCATAATGCA GCCAATCCTG 
CGCTTCCTGC AGCTGCTGTG CGAAAACCAC AATCGAGACC TGCAGAATTT TCTGCGCTGT 
CAGAACAATA AAAACAACTA TAATCTGGTG TGTGAGACGC TGCAGTTCCT GGACTGTATC 
TGTGGCAGCA CAACAGGAGG CCTGGGGCTG TTAGGACTCT ACATCAACCA GCATAACGTA 
GCGCTCATTA ATCAGACTGT AGAGAGCCTC ACTGAATACT GTCAAGGTCC CTGCCATGAC 
AATCAGAATT GCATTGCCAC CCATGAGTCT AATGGCATCG ACATCATTAT TGCACTGATT 
CTGAATGATA TTAATCCACT GGGCAGGAAG AGAATGGATC TGGTTCTAGA ACTAAAGAAT 
AATGCATCTA AGCTGCTGCT GGCCATCATG GAGAGCCGTC ATGACAGTGA GAATGCAGAA 
AGAATTCTGT ACAACATGAG ACCAAAAGAA CTAGTGGAGG TGATGAAAAT GGCCTACCAG 
CAGGGGGAGG CAGAGTTTGA GGATGAGGAA CAGGAGAATG GAGAGGATCA TGCAGCATCG 
CCTCGCAATG TTGGACACAA CATCTATATT CTTGCACATC AGCTGTCGCG TCACAATAAG 
GAATTGCAAA TACTACTGAA ACCAGGTGGA GAGGACCAGG CCCTGGAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itpr1a ( XM_005166095.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166152.1 Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X1, mRNA.

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CloneID	ODa12117
Clone ID Related Accession (Same CDS sequence)	XM_005166095.3 , XM_005166095.4
Accession Version	XM_005166095.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166095.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GGTAAATCCA 
GACTATGAAG AAGAATGCCT GGAGTCCCGC TCCTCTATGG ATTCGGAGCA TGAAGTGATT 
CGGGCTCGTG CACGTAACCC TCAGGATAAG GTGATGTACA CACTGGTGTC TGTTCCTGAT 
GGAAATGACA TATCCTCCAT CTTTGAGCTT GACCCTACAA CTTTGAGGGG CGGAGACTCA 
CTTGTGCCAA GAAACTCGTA TGTGAGGCTC AGGCATCTCT GCACCAACAC GTGGGTCCAC 
AGCACCAACC AGCCAATAGA TAAAGAGGAA GAGAAACCGG TCATGCTCAG AATAGGAACG 
TCTCCTCTGA AGGAAGATAA AGAGGCGTTT GCTATAGTAC CAGTCTCTCC AGCTGAGGTT 
CGAGATCTGG ATTTTGCTAA TGATGCTAGT AAAGTGTTGG CTTCCATTGC TGCCAAACTG 
GAGAAGGGCA CCATTACCCA GAATGAGAGG AGGTCAGTGA CGAAGCTGTT GGAGGATCTG 
GTGTATTTTG TGGTGGACAT ACCCAGCAGT GCTCAAGATG TGCTGGAGAT CACAGTCAAC 
AAACCCAACA GAGAGCGACA GAAACTCATG AGAGAGCAGA ACATTCTTAA ACAGATCTTC 
AAGCTGCTGC AGGCGCCATT TACAGACAGC GGGGACGGCC CTATGTTGCG ATTAGAAGAG 
CTCGCAGACC AGCGTCATGC ACCCTTTCGA CACATTTGCA GACTCTGTTA CAGAGTCCTA 
CGTTACTCAC AACAGGACTA TCGCAAAAAT CAGGAGTACA TAGCAAAGCA GTTCCGCTTC 
ATGCAGAAGC AGATAGGATA TGATGTGCTG GCTGAAGACA CAATTACAGC CCTTCTGCAC 
AACAACAGGA AGCTCCTAGA GAAACACATC ACAGCAGCTG AAATAGATAC CTTTGTCAGT 
CTGGTCCGCA AGAACAGAGA GCCCAGATTT CTGGATTACT TGTCAGATCT GTGTGTGTCG 
ATGAATAAAT CCATCCCGGT CACTCAAGAG CTCATCTGTA ATGCTGTGCT GGACCCAGCC 
AATGCAGACA TTCTCATCGA AACCAAGCTT GTGTTGTCAC GGTTTGAGGT GGCAGGAACG 
GTGTTGGGTG AAGGTGCGGA GGAGGAAGAG GAGGATGAAG AGGAGGTCTG GCTGTTCTGG 
AAGGACAGTG GAGGAGAGGT GAAGAGTAAA AGCATACGAG AGCTTGCACA GGACGCAAAA 
GATGGACATA CAGAGGACCA GGAAGTCATC AATTATTACA GATATCAGCT GAATTTGTTT 
GCTCGGATGT GTCTGGATCG ACAGTATCTT GCGATTAACA AGATATCAGC ACAGCTGGAT 
GTGGATCTGA TTCTGCGCTG CATGTCAGAT GAAGATTTGC CCTTTGACCT CAGAGCATCG 
TTCTGCCGCA TGATGCTGCA CATGCATGTA GACCGTGACC CTCAGGAGCA GGTCACCCCG 
GTCAAATATG CTCGACTGTG GTCTGAAATC CCATCACAAA TTTCCATTGA TGATTATGAT 
AATGATGGGA CGTCAAGTGA TGAAGTGAAA GAGCGCTTCT CTCAGACGAT GGAGTTTGTG 
GAGAATTACC TGAGGGATGT TGTCTGCCAG AGTTTCCCGT TCTCTGATAA GGAGAAAAAC 
AAACTCACTT TTGAGGTGGT GAACCTGGCA AGAAACCTGA TCTATTTTGG TTTCTATAAC 
TTCAGTGATC TGCTAAGGTT AACAAAAATC CTATTGGCAA TCCTGGACTG TGTCCACATA 
AGCACTCCCT TCCACATCAA GCTGGATAGA GAACCCGGTA AAGGAAGTAA CGTGATGCGC 
TCCATCCATG GGGTGGGAGA GCTGATGACA CAGGTGGTGT TGAGGGGTGG GGGATTTCTG 
CCAGCCTCCA GCCACAATCC TCCTGACAGA GACGAAGTGA AAGCACAAAG TGAACCTCAG 
AAACAGGACA TACTGGTGAT GGACACTAAA CTCAAGATCA TCGAGATCCT GCAGTTTATT 
CTGAATGTGC GGTTGGACTA CAGGATCTCC TGCCTCCTCT GTATCTTTAA GACAGAGTTT 
GATGAAAGTA ACTCTCAAAG TGAACCATCT TTAAGCCCAG AATCTCCTGC CAGCGTTCAA 
GGTGCGCTAG ACTTTGAGCA CATTGAAGAG CAGGCGGAGG GTATTTTTGG AGGAAGTAGT 
GAAGAGAACA CCCCGCTGGA TCTTGATGAT CATGGTGGAC GGACATTTCT GAGGGTCCTG 
CTTCACCTGA CCATGCATGA CTATCCTCCG CTGGTATCTC GAGCTCTTCA CCTGCTCTTC 
AGACACTTTA GCCAACGCCA GGAGGTCCTA CAGGCCTTCA AACAGGTTCA GCTCCTGGTG 
ACCAGTCAGG ATGTGGAAAA CTATAAACAG ATCAAGTCAG ATTTGGACCA GCTGAGGTCA 
ATAGTAGAGA AATCAGAGTT GTGGGTGTAT AAGAGACAAG GCTCAGACTC AGGACTGGAT 
GCTGGAGAAG TCACCCCTGA AGCCCACCAC AAGGGTGTCA TCAACTCGAG TAGATCAAAC 
AAACCCAAAG TGGAAAGTAC CAGCAGCTCG AACTACAGAG TCGTGAAAGA GATCCTGCTG 
AGGCTGAGCA AACTGTGTGT TTTGGAGGGC ATCTCTGGCA AGAAGAATAA GAAACAGCAG 
CAGCGGCTCC TCAGGAACAT GGGAGCTCAC AGTGTGGTCC TCGAACTGTT GCAAATCCCT 
TATGAGAAGG GGGAGGATGT CCAGATGCAG GAGATCATGA CCTTAGCACA TCAGTTCCTT 
CAAAACTTCT GTGCTGGAAA TCAGCAGAAC CAGGCACTTC TACACAAACA CATCAACCTG 
TTCCTCAACC CTGGGATCCT GGAGGCGGTG ACCATGCAGC ATATCTTCAT GAATAATTTC 
CAGTTGTGTA GTGAGATTAA TGAGCGGGTC GTGCAGCATT TTGTGCACTG CATCGAGACA 
CACGGCCGCA GCGTGCACTA CCTCAAGTTC CTGCAGACCA TTGTCAAGGC AGAGAACAAG 
TTCATCAAGA AATGCCAAGA CATCGTCATG GCTGAGCTGG TGACTGCAGG TGAGGATGTG 
CTGGTGTTCT ATAATGACCG GGCGTCTTTT CAGTCATTGG TTCAGATGAT GCGTCTTGAG 
CGGGAACGAC TTGATGAGAG CAGTGCACTC AGGTATCACA TTCACCTGGT GGAGTTGCTG 
GCTGTGTGCA CTGAAGGCAA GAACGTCTAC ACTGAGATCA AATGCAACTC GCTGCTCCCT 
CTCGATGATA TTGTACGTGT GGTCACACAT GAGGACTGCA TCCCTGAGGT GAAGATGGCC 
TACGTGAACT TCCTGAATCA CTGTTATGTT GATACAGAGG TTGAGATGAA GGAGATCTAC 
ACCAGCAATC ACATGTGGAA ACTTTTTGAT GATTTTCTGG TGGACGTCTG CAGAGTGTGC 
AATAACACAA GTGACCGTAA ACACGCTGAC ACAGTTCTGG AGAGATACGT TACAGAGACC 
ATAATGAGCA TCGTCACCAC CTTTTTCAGC TCACCATTCT CAGACCAGAG CACAAGTTTG 
CAGACCCGGC AGCCGGTGTT TGTGCAGCTG CTGCAGGGAG TGTTTCGTGT ATATCACTGC 
AACTGGCTTC TCCCAGGTCA GAAGGGGAAT GTGGAAGCCT GCATCAAAGT GCTGAGTGAC 
GTGGCCAAAG GCAGAGCCAT AGCCATTCCT GTGGATCTGG ACTGTCAGGT GAATAATCTG 
TTCATGAAGT CCAACAACAT TGTGCAGAAA ACAGCCCTCA GCTGGAGACT CTCTGTCCGT 
AACGCGACTC GGAGAGACTC TGTTCTGACC ACCTCTCGAG ACTATCGCAA CATCATTGAA 
AGACTACAGG ATATAGTGTC TGCATTGGAG GAGCGGTTGA GGCCTCTGGT TCAGGCTGAG 
TTGTCAGTGT TGGTGGATGT TCTCCATCGC CCAGAGCTGC TGTTTCCAGA GCACACTGAA 
GCCCACCGCA AGTGTGAGAG TGGAGGCTTC ATCTGCAAGT TGATAAAACA CACTAAGCAG 
CTTCTAGAAG AAAACGAAGA GAGGCTTTGC ATCAAAGTCC TTCAGACACT CCGAGAGATG 
ATGACCAAAG ACAGAGGATA TGGAGAGAAG CTTCTGGGAT ATGATGATGA GATGGATGTC 
ACGGAGGTGG TGGATATCAG TCTGCCACCC AAACAGCAGG AGGATCGGCA GAGGGGGGAG 
GCCCTTCGGC AGCTGCTCGT CAATCGTTAC TATGGCTTCA GGAGTGGTGG GCGGAGAGAA 
AGTCTGACCT CGTTTGGTAA CTCCACCCTA ACTCCTGTCG GGCCAATCAA GAGCCAGCCA 
GGGGGGTTGA GCAGGGCTGA GATGTCATTA ATGGAGGTGC AGTGTCATCT AGACCGTGAA 
GGGGCATCGG ATCTTGTCAT TGATCTCATC ATGAACACTA CCAGCGACCG CGTCTTCCAT 
GAAAGCATTC TGTTGGCCAT CGCACTGTTG GAGGGCGGAA ACACCATCAT ACAGCATTCC 
TTCTTCAAAC GTTTAACAGA AGACAAAAAT TCAGAGAAGT TTTTCAGGGT GTTTTATGAC 
AGAATGAAAG TGGCTCAGGT GGAGATCAAA GCCACGGTTA CTGTCAACAC TAGTGACCTC 
GGCAACAAGC GGCGTGACGA CAACACTCCA GACAAAGATA CTCCACAACG CCGCAGAGGT 
AAAGACTCCG GTGTGGTGGT GACAGATGAT GCTCGTGAGC AGCTGTTGGA GGCTTCAGCT 
GCGACTAAGA AGGCCTTTGG TTCATATCGT CGAGATGCTG ATCCCGAGGA GACGTTTGGC 
ACGGCTGATG GAGATAAAGG AGGTGGAGAT AAAGGCACAG AGCAGGGCGA GATGAGCCCT 
GTCATCCTCA TAATGCAGCC AATCCTGCGC TTCCTGCAGC TGCTGTGCGA AAACCACAAT 
CGAGACCTGC AGAATTTTCT GCGCTGTCAG AACAATAAAA ACAACTATAA TCTGGTGTGT 
GAGACGCTGC AGTTCCTGGA CTGTATCTGT GGCAGCACAA CAGGAGGCCT GGGGCTGTTA 
GGACTCTACA TCAACCAGCA TAACGTAGCG CTCATTAATC AGACTGTAGA GAGCCTCACT 
GAATACTGTC AAGGTCCCTG CCATGACAAT CAGAATTGCA TTGCCACCCA TGAGTCTAAT 
GGCATCGACA TCATTATTGC ACTGATTCTG AATGATATTA ATCCACTGGG CAGGAAGAGA 
ATGGATCTGG TTCTAGAACT AAAGAATAAT GCATCTAAGC TGCTGCTGGC CATCATGGAG 
AGCCGTCATG ACAGTGAGAA TGCAGAAAGA ATTCTGTACA ACATGAGACC AAAAGAACTA 
GTGGAGGTGA TGAAAATGGC CTACCAGCAG GGGGAGGCAG AGTTTGAGGA TGAGGAACAG 
GAGAATGGAG AGGATCATGC AGCATCGCCT CGCAATGTTG GACACAACAT CTATATTCTT 
GCACATCAGC TGTCGCGTCA CAATAAGGAA TTGCAAATAC TACTGAAACC AGGTGGAGAG 
GACCAGGCCC TGGAGTATTA CACAAAACAC ACCTCACAGA TAGAGATAGT TCGACAGGAT 
CGCACTATGG AACAGATTGT GTTTCCTGTA CCAAATATCT GCTCTTTTCT GACAAATGAG 
TCCAAGCTGC GTGTGTACTA TGGTACAGAG AGAGACGAGC AAGGCAGTAA AATAAATGAC 
TTCTTCTTGC ATGCAGACGA CCTCTTTAAT GAAATGAGGT GGCAGAAAAA ACTAAGAGCT 
CAGCCAGTGC TGTACTGGTG CAGCAGGAAC ATGTCGTTTT GGAGTAACGT TTCCTTTAAT 
CTGGCGGTGC TCATTAATTT GCTGGTGGCA TTTTTCTACC CTCTGGATGG AGTGAGCGAA 
AGCCAGCTGG AACCTTCTCT GTCTCTGTTG CTGTGGGTTT GTTTGTTGGG CTCACTGGGC 
TTCGTGTTGA TGTCCCCGAG GCCAAATGCA GTGCGGGTGC TGGTCATCTC CACTGTCCTG 
CAGTTGGGCT TCTCTGTAGG GCTGCAGCAC ATGCTCACAC TCCTGGGAGC TTTCAATGTT 
TGTAATAAGA TCGTGTTCAT GCTGAGCTTT GTGGGTAACC GTGGGTCATT CACCCGGGGT 
TATCGGGTGA TGGTCATGGA TAGAGAGTTC CTGTTTCATC TGCTGTACCT TCTCATCTGC 
ATACTGGGCC TTTTTGGACA CGTCTTCTTT TACAGTCTTC TGTTGTTTGA CCTGGTGAAC 
CGGGAAGAGA CTCTTCTGAA CGTCATCAAG AGTGTGACTC GAAACGGCCG CTCCATCGTC 
TTGACCGCAG TGCTGGGCCT CATCCTGGTC TACCTCTTTT CTATTGTTGG ATATATGTTT 
TTCAAAGATG ACTTCATCCT GGAGGTGAAC CGCATCTCCA ACGGCACTCT GGAGGAGGGG 
GTGACTTTAC CTGCTGGTTT TTGTGATGCG AGAAACGGGT CGTGTCTTTC TGATGATGTT 
GACGAGGATG ACGTGGAGCG CGCGTGTGAT TCTCTATGGA TGTGTATGAT CACTGTACTC 
AGTCATGGCC TCAGGAGCGG TGGAGGAGTT GGAGATGTCC TGCGCAAACC GTCTAAAGAG 
GAGCGTCTGT TCGCCGCACG GGTGGTGTAC GATCTCCTGT TCTTCTTCCT GGTGATCATC 
ATTGTGTTGA ATCTGATCTT CGGTGTGATC ATTGACACAT TTGCAGATCT GAGAAGCGAG 
AAGCAGAGAA AAGAGGAGAT CTTGAAGACC ACATGCTTCA TCTGTGGTCT GGAGAGAGAT 
AAGTTTGATA ATAAAACGGT GACGTTTGAA GAGCACATCA AGGAGGAGCA CAACCTGTGG 
CATTACCTCT TCTTCATAGT GCTGGTGCGG GTCAAAGACT CCACCGAATA CACGGGACCC 
GAGAGCTACG TCGCTCAGAT GATCAAGGAG CACAATCTGG ACTGGTTTCC TCGTATGAGG 
GCGATGTCTC TGGTCAGCAG TGATGGAGAA GGTGAACAGA ATGAACTGCG CAGCTTACAG 
GAGAAACTCG AGTCCACAAT GAGGCTCGTC TCTAATCTTA CCAATCAGCT CACAGAGCTC 
AAAGAGCAGA TGACTGAGCA AAGGAAACAC AAGCAGAGGC TCGGTCTGCT GGGAAACCCC 
GCCCACCTCA ACATCAACCC ACAGCAGCCA GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166152.1
CDS	148..8283
Translation	MVDKLSSFLHIGDVCSLYAEGSTSGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGTSTTDTVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDAAGNEGSWFYIQPFYKLRSIGDNVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCTTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDEHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEVNPDYEEECLESRSSMDSEHEVIRARARNPQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSPLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAAKLEKGTITQNERRSVTKLLEDLVYFVVDIPSSAQDVLEITVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRYSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDPANADILIETKLVLSRFEVAGTVLGEGAEEEEEDEEEVWLFWKDSGGEVKSKSIRELAQDAKDGHTEDQEVINYYRYQLNLFARMCLDRQYLAINKISAQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSQISIDDYDNDGTSSDEVKERFSQTMEFVENYLRDVVCQSFPFSDKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHISTPFHIKLDREPGKGSNVMRSIHGVGELMTQVVLRGGGFLPASSHNPPDRDEVKAQSEPQKQDILVMDTKLKIIEILQFILNVRLDYRISCLLCIFKTEFDESNSQSEPSLSPESPASVQGALDFEHIEEQAEGIFGGSSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALHLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSDSGLDAGEVTPEAHHKGVINSSRSNKPKVESTSSSNYRVVKEILLRLSKLCVLEGISGKKNKKQQQRLLRNMGAHSVVLELLQIPYEKGEDVQMQEIMTLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAVTMQHIFMNNFQLCSEINERVVQHFVHCIETHGRSVHYLKFLQTIVKAENKFIKKCQDIVMAELVTAGEDVLVFYNDRASFQSLVQMMRLERERLDESSALRYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKMAYVNFLNHCYVDTEVEMKEIYTSNHMWKLFDDFLVDVCRVCNNTSDRKHADTVLERYVTETIMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQGVFRVYHCNWLLPGQKGNVEACIKVLSDVAKGRAIAIPVDLDCQVNNLFMKSNNIVQKTALSWRLSVRNATRRDSVLTTSRDYRNIIERLQDIVSALEERLRPLVQAELSVLVDVLHRPELLFPEHTEAHRKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKLLGYDDEMDVTEVVDISLPPKQQEDRQRGEALRQLLVNRYYGFRSGGRRESLTSFGNSTLTPVGPIKSQPGGLSRAEMSLMEVQCHLDREGASDLVIDLIMNTTSDRVFHESILLAIALLEGGNTIIQHSFFKRLTEDKNSEKFFRVFYDRMKVAQVEIKATVTVNTSDLGNKRRDDNTPDKDTPQRRRGKDSGVVVTDDAREQLLEASAATKKAFGSYRRDADPEETFGTADGDKGGGDKGTEQGEMSPVILIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINQHNVALINQTVESLTEYCQGPCHDNQNCIATHESNGIDIIIALILNDINPLGRKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVMKMAYQQGEAEFEDEEQENGEDHAASPRNVGHNIYILAHQLSRHNKELQILLKPGGEDQALEYYTKHTSQIEIVRQDRTMEQIVFPVPNICSFLTNESKLRVYYGTERDEQGSKINDFFLHADDLFNEMRWQKKLRAQPVLYWCSRNMSFWSNVSFNLAVLINLLVAFFYPLDGVSESQLEPSLSLLLWVCLLGSLGFVLMSPRPNAVRVLVISTVLQLGFSVGLQHMLTLLGAFNVCNKIVFMLSFVGNRGSFTRGYRVMVMDREFLFHLLYLLICILGLFGHVFFYSLLLFDLVNREETLLNVIKSVTRNGRSIVLTAVLGLILVYLFSIVGYMFFKDDFILEVNRISNGTLEEGVTLPAGFCDARNGSCLSDDVDEDDVERACDSLWMCMITVLSHGLRSGGGVGDVLRKPSKEERLFAARVVYDLLFFFLVIIIVLNLIFGVIIDTFADLRSEKQRKEEILKTTCFICGLERDKFDNKTVTFEEHIKEEHNLWHYLFFIVLVRVKDSTEYTGPESYVAQMIKEHNLDWFPRMRAMSLVSSDGEGEQNELRSLQEKLESTMRLVSNLTNQLTELKEQMTEQRKHKQRLGLLGNPAHLNINPQQPA

Target ORF information:

RefSeq Version	XM_005166095.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166095.3

ORF Insert Sequence:

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GGTAAATCCA 
GACTATGAAG AAGAATGCCT GGAGTCCCGC TCCTCTATGG ATTCGGAGCA TGAAGTGATT 
CGGGCTCGTG CACGTAACCC TCAGGATAAG GTGATGTACA CACTGGTGTC TGTTCCTGAT 
GGAAATGACA TATCCTCCAT CTTTGAGCTT GACCCTACAA CTTTGAGGGG CGGAGACTCA 
CTTGTGCCAA GAAACTCGTA TGTGAGGCTC AGGCATCTCT GCACCAACAC GTGGGTCCAC 
AGCACCAACC AGCCAATAGA TAAAGAGGAA GAGAAACCGG TCATGCTCAG AATAGGAACG 
TCTCCTCTGA AGGAAGATAA AGAGGCGTTT GCTATAGTAC CAGTCTCTCC AGCTGAGGTT 
CGAGATCTGG ATTTTGCTAA TGATGCTAGT AAAGTGTTGG CTTCCATTGC TGCCAAACTG 
GAGAAGGGCA CCATTACCCA GAATGAGAGG AGGTCAGTGA CGAAGCTGTT GGAGGATCTG 
GTGTATTTTG TGGTGGACAT ACCCAGCAGT GCTCAAGATG TGCTGGAGAT CACAGTCAAC 
AAACCCAACA GAGAGCGACA GAAACTCATG AGAGAGCAGA ACATTCTTAA ACAGATCTTC 
AAGCTGCTGC AGGCGCCATT TACAGACAGC GGGGACGGCC CTATGTTGCG ATTAGAAGAG 
CTCGCAGACC AGCGTCATGC ACCCTTTCGA CACATTTGCA GACTCTGTTA CAGAGTCCTA 
CGTTACTCAC AACAGGACTA TCGCAAAAAT CAGGAGTACA TAGCAAAGCA GTTCCGCTTC 
ATGCAGAAGC AGATAGGATA TGATGTGCTG GCTGAAGACA CAATTACAGC CCTTCTGCAC 
AACAACAGGA AGCTCCTAGA GAAACACATC ACAGCAGCTG AAATAGATAC CTTTGTCAGT 
CTGGTCCGCA AGAACAGAGA GCCCAGATTT CTGGATTACT TGTCAGATCT GTGTGTGTCG 
ATGAATAAAT CCATCCCGGT CACTCAAGAG CTCATCTGTA ATGCTGTGCT GGACCCAGCC 
AATGCAGACA TTCTCATCGA AACCAAGCTT GTGTTGTCAC GGTTTGAGGT GGCAGGAACG 
GTGTTGGGTG AAGGTGCGGA GGAGGAAGAG GAGGATGAAG AGGAGGTCTG GCTGTTCTGG 
AAGGACAGTG GAGGAGAGGT GAAGAGTAAA AGCATACGAG AGCTTGCACA GGACGCAAAA 
GATGGACATA CAGAGGACCA GGAAGTCATC AATTATTACA GATATCAGCT GAATTTGTTT 
GCTCGGATGT GTCTGGATCG ACAGTATCTT GCGATTAACA AGATATCAGC ACAGCTGGAT 
GTGGATCTGA TTCTGCGCTG CATGTCAGAT GAAGATTTGC CCTTTGACCT CAGAGCATCG 
TTCTGCCGCA TGATGCTGCA CATGCATGTA GACCGTGACC CTCAGGAGCA GGTCACCCCG 
GTCAAATATG CTCGACTGTG GTCTGAAATC CCATCACAAA TTTCCATTGA TGATTATGAT 
AATGATGGGA CGTCAAGTGA TGAAGTGAAA GAGCGCTTCT CTCAGACGAT GGAGTTTGTG 
GAGAATTACC TGAGGGATGT TGTCTGCCAG AGTTTCCCGT TCTCTGATAA GGAGAAAAAC 
AAACTCACTT TTGAGGTGGT GAACCTGGCA AGAAACCTGA TCTATTTTGG TTTCTATAAC 
TTCAGTGATC TGCTAAGGTT AACAAAAATC CTATTGGCAA TCCTGGACTG TGTCCACATA 
AGCACTCCCT TCCACATCAA GCTGGATAGA GAACCCGGTA AAGGAAGTAA CGTGATGCGC 
TCCATCCATG GGGTGGGAGA GCTGATGACA CAGGTGGTGT TGAGGGGTGG GGGATTTCTG 
CCAGCCTCCA GCCACAATCC TCCTGACAGA GACGAAGTGA AAGCACAAAG TGAACCTCAG 
AAACAGGACA TACTGGTGAT GGACACTAAA CTCAAGATCA TCGAGATCCT GCAGTTTATT 
CTGAATGTGC GGTTGGACTA CAGGATCTCC TGCCTCCTCT GTATCTTTAA GACAGAGTTT 
GATGAAAGTA ACTCTCAAAG TGAACCATCT TTAAGCCCAG AATCTCCTGC CAGCGTTCAA 
GGTGCGCTAG ACTTTGAGCA CATTGAAGAG CAGGCGGAGG GTATTTTTGG AGGAAGTAGT 
GAAGAGAACA CCCCGCTGGA TCTTGATGAT CATGGTGGAC GGACATTTCT GAGGGTCCTG 
CTTCACCTGA CCATGCATGA CTATCCTCCG CTGGTATCTC GAGCTCTTCA CCTGCTCTTC 
AGACACTTTA GCCAACGCCA GGAGGTCCTA CAGGCCTTCA AACAGGTTCA GCTCCTGGTG 
ACCAGTCAGG ATGTGGAAAA CTATAAACAG ATCAAGTCAG ATTTGGACCA GCTGAGGTCA 
ATAGTAGAGA AATCAGAGTT GTGGGTGTAT AAGAGACAAG GCTCAGACTC AGGACTGGAT 
GCTGGAGAAG TCACCCCTGA AGCCCACCAC AAGGGTGTCA TCAACTCGAG TAGATCAAAC 
AAACCCAAAG TGGAAAGTAC CAGCAGCTCG AACTACAGAG TCGTGAAAGA GATCCTGCTG 
AGGCTGAGCA AACTGTGTGT TTTGGAGGGC ATCTCTGGCA AGAAGAATAA GAAACAGCAG 
CAGCGGCTCC TCAGGAACAT GGGAGCTCAC AGTGTGGTCC TCGAACTGTT GCAAATCCCT 
TATGAGAAGG GGGAGGATGT CCAGATGCAG GAGATCATGA CCTTAGCACA TCAGTTCCTT 
CAAAACTTCT GTGCTGGAAA TCAGCAGAAC CAGGCACTTC TACACAAACA CATCAACCTG 
TTCCTCAACC CTGGGATCCT GGAGGCGGTG ACCATGCAGC ATATCTTCAT GAATAATTTC 
CAGTTGTGTA GTGAGATTAA TGAGCGGGTC GTGCAGCATT TTGTGCACTG CATCGAGACA 
CACGGCCGCA GCGTGCACTA CCTCAAGTTC CTGCAGACCA TTGTCAAGGC AGAGAACAAG 
TTCATCAAGA AATGCCAAGA CATCGTCATG GCTGAGCTGG TGACTGCAGG TGAGGATGTG 
CTGGTGTTCT ATAATGACCG GGCGTCTTTT CAGTCATTGG TTCAGATGAT GCGTCTTGAG 
CGGGAACGAC TTGATGAGAG CAGTGCACTC AGGTATCACA TTCACCTGGT GGAGTTGCTG 
GCTGTGTGCA CTGAAGGCAA GAACGTCTAC ACTGAGATCA AATGCAACTC GCTGCTCCCT 
CTCGATGATA TTGTACGTGT GGTCACACAT GAGGACTGCA TCCCTGAGGT GAAGATGGCC 
TACGTGAACT TCCTGAATCA CTGTTATGTT GATACAGAGG TTGAGATGAA GGAGATCTAC 
ACCAGCAATC ACATGTGGAA ACTTTTTGAT GATTTTCTGG TGGACGTCTG CAGAGTGTGC 
AATAACACAA GTGACCGTAA ACACGCTGAC ACAGTTCTGG AGAGATACGT TACAGAGACC 
ATAATGAGCA TCGTCACCAC CTTTTTCAGC TCACCATTCT CAGACCAGAG CACAAGTTTG 
CAGACCCGGC AGCCGGTGTT TGTGCAGCTG CTGCAGGGAG TGTTTCGTGT ATATCACTGC 
AACTGGCTTC TCCCAGGTCA GAAGGGGAAT GTGGAAGCCT GCATCAAAGT GCTGAGTGAC 
GTGGCCAAAG GCAGAGCCAT AGCCATTCCT GTGGATCTGG ACTGTCAGGT GAATAATCTG 
TTCATGAAGT CCAACAACAT TGTGCAGAAA ACAGCCCTCA GCTGGAGACT CTCTGTCCGT 
AACGCGACTC GGAGAGACTC TGTTCTGACC ACCTCTCGAG ACTATCGCAA CATCATTGAA 
AGACTACAGG ATATAGTGTC TGCATTGGAG GAGCGGTTGA GGCCTCTGGT TCAGGCTGAG 
TTGTCAGTGT TGGTGGATGT TCTCCATCGC CCAGAGCTGC TGTTTCCAGA GCACACTGAA 
GCCCACCGCA AGTGTGAGAG TGGAGGCTTC ATCTGCAAGT TGATAAAACA CACTAAGCAG 
CTTCTAGAAG AAAACGAAGA GAGGCTTTGC ATCAAAGTCC TTCAGACACT CCGAGAGATG 
ATGACCAAAG ACAGAGGATA TGGAGAGAAG CTTCTGGGAT ATGATGATGA GATGGATGTC 
ACGGAGGTGG TGGATATCAG TCTGCCACCC AAACAGCAGG AGGATCGGCA GAGGGGGGAG 
GCCCTTCGGC AGCTGCTCGT CAATCGTTAC TATGGCTTCA GGAGTGGTGG GCGGAGAGAA 
AGTCTGACCT CGTTTGGTAA CTCCACCCTA ACTCCTGTCG GGCCAATCAA GAGCCAGCCA 
GGGGGGTTGA GCAGGGCTGA GATGTCATTA ATGGAGGTGC AGTGTCATCT AGACCGTGAA 
GGGGCATCGG ATCTTGTCAT TGATCTCATC ATGAACACTA CCAGCGACCG CGTCTTCCAT 
GAAAGCATTC TGTTGGCCAT CGCACTGTTG GAGGGCGGAA ACACCATCAT ACAGCATTCC 
TTCTTCAAAC GTTTAACAGA AGACAAAAAT TCAGAGAAGT TTTTCAGGGT GTTTTATGAC 
AGAATGAAAG TGGCTCAGGT GGAGATCAAA GCCACGGTTA CTGTCAACAC TAGTGACCTC 
GGCAACAAGC GGCGTGACGA CAACACTCCA GACAAAGATA CTCCACAACG CCGCAGAGGT 
AAAGACTCCG GTGTGGTGGT GACAGATGAT GCTCGTGAGC AGCTGTTGGA GGCTTCAGCT 
GCGACTAAGA AGGCCTTTGG TTCATATCGT CGAGATGCTG ATCCCGAGGA GACGTTTGGC 
ACGGCTGATG GAGATAAAGG AGGTGGAGAT AAAGGCACAG AGCAGGGCGA GATGAGCCCT 
GTCATCCTCA TAATGCAGCC AATCCTGCGC TTCCTGCAGC TGCTGTGCGA AAACCACAAT 
CGAGACCTGC AGAATTTTCT GCGCTGTCAG AACAATAAAA ACAACTATAA TCTGGTGTGT 
GAGACGCTGC AGTTCCTGGA CTGTATCTGT GGCAGCACAA CAGGAGGCCT GGGGCTGTTA 
GGACTCTACA TCAACCAGCA TAACGTAGCG CTCATTAATC AGACTGTAGA GAGCCTCACT 
GAATACTGTC AAGGTCCCTG CCATGACAAT CAGAATTGCA TTGCCACCCA TGAGTCTAAT 
GGCATCGACA TCATTATTGC ACTGATTCTG AATGATATTA ATCCACTGGG CAGGAAGAGA 
ATGGATCTGG TTCTAGAACT AAAGAATAAT GCATCTAAGC TGCTGCTGGC CATCATGGAG 
AGCCGTCATG ACAGTGAGAA TGCAGAAAGA ATTCTGTACA ACATGAGACC AAAAGAACTA 
GTGGAGGTGA TGAAAATGGC CTACCAGCAG GGGGAGGCAG AGTTTGAGGA TGAGGAACAG 
GAGAATGGAG AGGATCATGC AGCATCGCCT CGCAATGTTG GACACAACAT CTATATTCTT 
GCACATCAGC TGTCGCGTCA CAATAAGGAA TTGCAAATAC TACTGAAACC AGGTGGAGAG 
GACCAGGCCC TGGAGTATTA CACAAAACAC ACCTCACAGA TAGAGATAGT TCGACAGGAT 
CGCACTATGG AACAGATTGT GTTTCCTGTA CCAAATATCT GCTCTTTTCT GACAAATGAG 
TCCAAGCTGC GTGTGTACTA TGGTACAGAG AGAGACGAGC AAGGCAGTAA AATAAATGAC 
TTCTTCTTGC ATGCAGACGA CCTCTTTAAT GAAATGAGGT GGCAGAAAAA ACTAAGAGCT 
CAGCCAGTGC TGTACTGGTG CAGCAGGAAC ATGTCGTTTT GGAGTAACGT TTCCTTTAAT 
CTGGCGGTGC TCATTAATTT GCTGGTGGCA TTTTTCTACC CTCTGGATGG AGTGAGCGAA 
AGCCAGCTGG AACCTTCTCT GTCTCTGTTG CTGTGGGTTT GTTTGTTGGG CTCACTGGGC 
TTCGTGTTGA TGTCCCCGAG GCCAAATGCA GTGCGGGTGC TGGTCATCTC CACTGTCCTG 
CAGTTGGGCT TCTCTGTAGG GCTGCAGCAC ATGCTCACAC TCCTGGGAGC TTTCAATGTT 
TGTAATAAGA TCGTGTTCAT GCTGAGCTTT GTGGGTAACC GTGGGTCATT CACCCGGGGT 
TATCGGGTGA TGGTCATGGA TAGAGAGTTC CTGTTTCATC TGCTGTACCT TCTCATCTGC 
ATACTGGGCC TTTTTGGACA CGTCTTCTTT TACAGTCTTC TGTTGTTTGA CCTGGTGAAC 
CGGGAAGAGA CTCTTCTGAA CGTCATCAAG AGTGTGACTC GAAACGGCCG CTCCATCGTC 
TTGACCGCAG TGCTGGGCCT CATCCTGGTC TACCTCTTTT CTATTGTTGG ATATATGTTT 
TTCAAAGATG ACTTCATCCT GGAGGTGAAC CGCATCTCCA ACGGCACTCT GGAGGAGGGG 
GTGACTTTAC CTGCTGGTTT TTGTGATGCG AGAAACGGGT CGTGTCTTTC TGATGATGTT 
GACGAGGATG ACGTGGAGCG CGCGTGTGAT TCTCTATGGA TGTGTATGAT CACTGTACTC 
AGTCATGGCC TCAGGAGCGG TGGAGGAGTT GGAGATGTCC TGCGCAAACC GTCTAAAGAG 
GAGCGTCTGT TCGCCGCACG GGTGGTGTAC GATCTCCTGT TCTTCTTCCT GGTGATCATC 
ATTGTGTTGA ATCTGATCTT CGGTGTGATC ATTGACACAT TTGCAGATCT GAGAAGCGAG 
AAGCAGAGAA AAGAGGAGAT CTTGAAGACC ACATGCTTCA TCTGTGGTCT GGAGAGAGAT 
AAGTTTGATA ATAAAACGGT GACGTTTGAA GAGCACATCA AGGAGGAGCA CAACCTGTGG 
CATTACCTCT TCTTCATAGT GCTGGTGCGG GTCAAAGACT CCACCGAATA CACGGGACCC 
GAGAGCTACG TCGCTCAGAT GATCAAGGAG CACAATCTGG ACTGGTTTCC TCGTATGAGG 
GCGATGTCTC TGGTCAGCAG TGATGGAGAA GGTGAACAGA ATGAACTGCG CAGCTTACAG 
GAGAAACTCG AGTCCACAAT GAGGCTCGTC TCTAATCTTA CCAATCAGCT CACAGAGCTC 
AAAGAGCAGA TGACTGAGCA AAGGAAACAC AAGCAGAGGC TCGGTCTGCT GGGAAACCCC 
GCCCACCTCA ACATCAACCC ACAGCAGCCA GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itpr1a ( XM_005166095.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005166152.1 Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X1, mRNA.

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CloneID	ODa12117
Clone ID Related Accession (Same CDS sequence)	XM_005166095.3 , XM_005166095.4
Accession Version	XM_005166095.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166095.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GGTAAATCCA 
GACTATGAAG AAGAATGCCT GGAGTCCCGC TCCTCTATGG ATTCGGAGCA TGAAGTGATT 
CGGGCTCGTG CACGTAACCC TCAGGATAAG GTGATGTACA CACTGGTGTC TGTTCCTGAT 
GGAAATGACA TATCCTCCAT CTTTGAGCTT GACCCTACAA CTTTGAGGGG CGGAGACTCA 
CTTGTGCCAA GAAACTCGTA TGTGAGGCTC AGGCATCTCT GCACCAACAC GTGGGTCCAC 
AGCACCAACC AGCCAATAGA TAAAGAGGAA GAGAAACCGG TCATGCTCAG AATAGGAACG 
TCTCCTCTGA AGGAAGATAA AGAGGCGTTT GCTATAGTAC CAGTCTCTCC AGCTGAGGTT 
CGAGATCTGG ATTTTGCTAA TGATGCTAGT AAAGTGTTGG CTTCCATTGC TGCCAAACTG 
GAGAAGGGCA CCATTACCCA GAATGAGAGG AGGTCAGTGA CGAAGCTGTT GGAGGATCTG 
GTGTATTTTG TGGTGGACAT ACCCAGCAGT GCTCAAGATG TGCTGGAGAT CACAGTCAAC 
AAACCCAACA GAGAGCGACA GAAACTCATG AGAGAGCAGA ACATTCTTAA ACAGATCTTC 
AAGCTGCTGC AGGCGCCATT TACAGACAGC GGGGACGGCC CTATGTTGCG ATTAGAAGAG 
CTCGCAGACC AGCGTCATGC ACCCTTTCGA CACATTTGCA GACTCTGTTA CAGAGTCCTA 
CGTTACTCAC AACAGGACTA TCGCAAAAAT CAGGAGTACA TAGCAAAGCA GTTCCGCTTC 
ATGCAGAAGC AGATAGGATA TGATGTGCTG GCTGAAGACA CAATTACAGC CCTTCTGCAC 
AACAACAGGA AGCTCCTAGA GAAACACATC ACAGCAGCTG AAATAGATAC CTTTGTCAGT 
CTGGTCCGCA AGAACAGAGA GCCCAGATTT CTGGATTACT TGTCAGATCT GTGTGTGTCG 
ATGAATAAAT CCATCCCGGT CACTCAAGAG CTCATCTGTA ATGCTGTGCT GGACCCAGCC 
AATGCAGACA TTCTCATCGA AACCAAGCTT GTGTTGTCAC GGTTTGAGGT GGCAGGAACG 
GTGTTGGGTG AAGGTGCGGA GGAGGAAGAG GAGGATGAAG AGGAGGTCTG GCTGTTCTGG 
AAGGACAGTG GAGGAGAGGT GAAGAGTAAA AGCATACGAG AGCTTGCACA GGACGCAAAA 
GATGGACATA CAGAGGACCA GGAAGTCATC AATTATTACA GATATCAGCT GAATTTGTTT 
GCTCGGATGT GTCTGGATCG ACAGTATCTT GCGATTAACA AGATATCAGC ACAGCTGGAT 
GTGGATCTGA TTCTGCGCTG CATGTCAGAT GAAGATTTGC CCTTTGACCT CAGAGCATCG 
TTCTGCCGCA TGATGCTGCA CATGCATGTA GACCGTGACC CTCAGGAGCA GGTCACCCCG 
GTCAAATATG CTCGACTGTG GTCTGAAATC CCATCACAAA TTTCCATTGA TGATTATGAT 
AATGATGGGA CGTCAAGTGA TGAAGTGAAA GAGCGCTTCT CTCAGACGAT GGAGTTTGTG 
GAGAATTACC TGAGGGATGT TGTCTGCCAG AGTTTCCCGT TCTCTGATAA GGAGAAAAAC 
AAACTCACTT TTGAGGTGGT GAACCTGGCA AGAAACCTGA TCTATTTTGG TTTCTATAAC 
TTCAGTGATC TGCTAAGGTT AACAAAAATC CTATTGGCAA TCCTGGACTG TGTCCACATA 
AGCACTCCCT TCCACATCAA GCTGGATAGA GAACCCGGTA AAGGAAGTAA CGTGATGCGC 
TCCATCCATG GGGTGGGAGA GCTGATGACA CAGGTGGTGT TGAGGGGTGG GGGATTTCTG 
CCAGCCTCCA GCCACAATCC TCCTGACAGA GACGAAGTGA AAGCACAAAG TGAACCTCAG 
AAACAGGACA TACTGGTGAT GGACACTAAA CTCAAGATCA TCGAGATCCT GCAGTTTATT 
CTGAATGTGC GGTTGGACTA CAGGATCTCC TGCCTCCTCT GTATCTTTAA GACAGAGTTT 
GATGAAAGTA ACTCTCAAAG TGAACCATCT TTAAGCCCAG AATCTCCTGC CAGCGTTCAA 
GGTGCGCTAG ACTTTGAGCA CATTGAAGAG CAGGCGGAGG GTATTTTTGG AGGAAGTAGT 
GAAGAGAACA CCCCGCTGGA TCTTGATGAT CATGGTGGAC GGACATTTCT GAGGGTCCTG 
CTTCACCTGA CCATGCATGA CTATCCTCCG CTGGTATCTC GAGCTCTTCA CCTGCTCTTC 
AGACACTTTA GCCAACGCCA GGAGGTCCTA CAGGCCTTCA AACAGGTTCA GCTCCTGGTG 
ACCAGTCAGG ATGTGGAAAA CTATAAACAG ATCAAGTCAG ATTTGGACCA GCTGAGGTCA 
ATAGTAGAGA AATCAGAGTT GTGGGTGTAT AAGAGACAAG GCTCAGACTC AGGACTGGAT 
GCTGGAGAAG TCACCCCTGA AGCCCACCAC AAGGGTGTCA TCAACTCGAG TAGATCAAAC 
AAACCCAAAG TGGAAAGTAC CAGCAGCTCG AACTACAGAG TCGTGAAAGA GATCCTGCTG 
AGGCTGAGCA AACTGTGTGT TTTGGAGGGC ATCTCTGGCA AGAAGAATAA GAAACAGCAG 
CAGCGGCTCC TCAGGAACAT GGGAGCTCAC AGTGTGGTCC TCGAACTGTT GCAAATCCCT 
TATGAGAAGG GGGAGGATGT CCAGATGCAG GAGATCATGA CCTTAGCACA TCAGTTCCTT 
CAAAACTTCT GTGCTGGAAA TCAGCAGAAC CAGGCACTTC TACACAAACA CATCAACCTG 
TTCCTCAACC CTGGGATCCT GGAGGCGGTG ACCATGCAGC ATATCTTCAT GAATAATTTC 
CAGTTGTGTA GTGAGATTAA TGAGCGGGTC GTGCAGCATT TTGTGCACTG CATCGAGACA 
CACGGCCGCA GCGTGCACTA CCTCAAGTTC CTGCAGACCA TTGTCAAGGC AGAGAACAAG 
TTCATCAAGA AATGCCAAGA CATCGTCATG GCTGAGCTGG TGACTGCAGG TGAGGATGTG 
CTGGTGTTCT ATAATGACCG GGCGTCTTTT CAGTCATTGG TTCAGATGAT GCGTCTTGAG 
CGGGAACGAC TTGATGAGAG CAGTGCACTC AGGTATCACA TTCACCTGGT GGAGTTGCTG 
GCTGTGTGCA CTGAAGGCAA GAACGTCTAC ACTGAGATCA AATGCAACTC GCTGCTCCCT 
CTCGATGATA TTGTACGTGT GGTCACACAT GAGGACTGCA TCCCTGAGGT GAAGATGGCC 
TACGTGAACT TCCTGAATCA CTGTTATGTT GATACAGAGG TTGAGATGAA GGAGATCTAC 
ACCAGCAATC ACATGTGGAA ACTTTTTGAT GATTTTCTGG TGGACGTCTG CAGAGTGTGC 
AATAACACAA GTGACCGTAA ACACGCTGAC ACAGTTCTGG AGAGATACGT TACAGAGACC 
ATAATGAGCA TCGTCACCAC CTTTTTCAGC TCACCATTCT CAGACCAGAG CACAAGTTTG 
CAGACCCGGC AGCCGGTGTT TGTGCAGCTG CTGCAGGGAG TGTTTCGTGT ATATCACTGC 
AACTGGCTTC TCCCAGGTCA GAAGGGGAAT GTGGAAGCCT GCATCAAAGT GCTGAGTGAC 
GTGGCCAAAG GCAGAGCCAT AGCCATTCCT GTGGATCTGG ACTGTCAGGT GAATAATCTG 
TTCATGAAGT CCAACAACAT TGTGCAGAAA ACAGCCCTCA GCTGGAGACT CTCTGTCCGT 
AACGCGACTC GGAGAGACTC TGTTCTGACC ACCTCTCGAG ACTATCGCAA CATCATTGAA 
AGACTACAGG ATATAGTGTC TGCATTGGAG GAGCGGTTGA GGCCTCTGGT TCAGGCTGAG 
TTGTCAGTGT TGGTGGATGT TCTCCATCGC CCAGAGCTGC TGTTTCCAGA GCACACTGAA 
GCCCACCGCA AGTGTGAGAG TGGAGGCTTC ATCTGCAAGT TGATAAAACA CACTAAGCAG 
CTTCTAGAAG AAAACGAAGA GAGGCTTTGC ATCAAAGTCC TTCAGACACT CCGAGAGATG 
ATGACCAAAG ACAGAGGATA TGGAGAGAAG CTTCTGGGAT ATGATGATGA GATGGATGTC 
ACGGAGGTGG TGGATATCAG TCTGCCACCC AAACAGCAGG AGGATCGGCA GAGGGGGGAG 
GCCCTTCGGC AGCTGCTCGT CAATCGTTAC TATGGCTTCA GGAGTGGTGG GCGGAGAGAA 
AGTCTGACCT CGTTTGGTAA CTCCACCCTA ACTCCTGTCG GGCCAATCAA GAGCCAGCCA 
GGGGGGTTGA GCAGGGCTGA GATGTCATTA ATGGAGGTGC AGTGTCATCT AGACCGTGAA 
GGGGCATCGG ATCTTGTCAT TGATCTCATC ATGAACACTA CCAGCGACCG CGTCTTCCAT 
GAAAGCATTC TGTTGGCCAT CGCACTGTTG GAGGGCGGAA ACACCATCAT ACAGCATTCC 
TTCTTCAAAC GTTTAACAGA AGACAAAAAT TCAGAGAAGT TTTTCAGGGT GTTTTATGAC 
AGAATGAAAG TGGCTCAGGT GGAGATCAAA GCCACGGTTA CTGTCAACAC TAGTGACCTC 
GGCAACAAGC GGCGTGACGA CAACACTCCA GACAAAGATA CTCCACAACG CCGCAGAGGT 
AAAGACTCCG GTGTGGTGGT GACAGATGAT GCTCGTGAGC AGCTGTTGGA GGCTTCAGCT 
GCGACTAAGA AGGCCTTTGG TTCATATCGT CGAGATGCTG ATCCCGAGGA GACGTTTGGC 
ACGGCTGATG GAGATAAAGG AGGTGGAGAT AAAGGCACAG AGCAGGGCGA GATGAGCCCT 
GTCATCCTCA TAATGCAGCC AATCCTGCGC TTCCTGCAGC TGCTGTGCGA AAACCACAAT 
CGAGACCTGC AGAATTTTCT GCGCTGTCAG AACAATAAAA ACAACTATAA TCTGGTGTGT 
GAGACGCTGC AGTTCCTGGA CTGTATCTGT GGCAGCACAA CAGGAGGCCT GGGGCTGTTA 
GGACTCTACA TCAACCAGCA TAACGTAGCG CTCATTAATC AGACTGTAGA GAGCCTCACT 
GAATACTGTC AAGGTCCCTG CCATGACAAT CAGAATTGCA TTGCCACCCA TGAGTCTAAT 
GGCATCGACA TCATTATTGC ACTGATTCTG AATGATATTA ATCCACTGGG CAGGAAGAGA 
ATGGATCTGG TTCTAGAACT AAAGAATAAT GCATCTAAGC TGCTGCTGGC CATCATGGAG 
AGCCGTCATG ACAGTGAGAA TGCAGAAAGA ATTCTGTACA ACATGAGACC AAAAGAACTA 
GTGGAGGTGA TGAAAATGGC CTACCAGCAG GGGGAGGCAG AGTTTGAGGA TGAGGAACAG 
GAGAATGGAG AGGATCATGC AGCATCGCCT CGCAATGTTG GACACAACAT CTATATTCTT 
GCACATCAGC TGTCGCGTCA CAATAAGGAA TTGCAAATAC TACTGAAACC AGGTGGAGAG 
GACCAGGCCC TGGAGTATTA CACAAAACAC ACCTCACAGA TAGAGATAGT TCGACAGGAT 
CGCACTATGG AACAGATTGT GTTTCCTGTA CCAAATATCT GCTCTTTTCT GACAAATGAG 
TCCAAGCTGC GTGTGTACTA TGGTACAGAG AGAGACGAGC AAGGCAGTAA AATAAATGAC 
TTCTTCTTGC ATGCAGACGA CCTCTTTAAT GAAATGAGGT GGCAGAAAAA ACTAAGAGCT 
CAGCCAGTGC TGTACTGGTG CAGCAGGAAC ATGTCGTTTT GGAGTAACGT TTCCTTTAAT 
CTGGCGGTGC TCATTAATTT GCTGGTGGCA TTTTTCTACC CTCTGGATGG AGTGAGCGAA 
AGCCAGCTGG AACCTTCTCT GTCTCTGTTG CTGTGGGTTT GTTTGTTGGG CTCACTGGGC 
TTCGTGTTGA TGTCCCCGAG GCCAAATGCA GTGCGGGTGC TGGTCATCTC CACTGTCCTG 
CAGTTGGGCT TCTCTGTAGG GCTGCAGCAC ATGCTCACAC TCCTGGGAGC TTTCAATGTT 
TGTAATAAGA TCGTGTTCAT GCTGAGCTTT GTGGGTAACC GTGGGTCATT CACCCGGGGT 
TATCGGGTGA TGGTCATGGA TAGAGAGTTC CTGTTTCATC TGCTGTACCT TCTCATCTGC 
ATACTGGGCC TTTTTGGACA CGTCTTCTTT TACAGTCTTC TGTTGTTTGA CCTGGTGAAC 
CGGGAAGAGA CTCTTCTGAA CGTCATCAAG AGTGTGACTC GAAACGGCCG CTCCATCGTC 
TTGACCGCAG TGCTGGGCCT CATCCTGGTC TACCTCTTTT CTATTGTTGG ATATATGTTT 
TTCAAAGATG ACTTCATCCT GGAGGTGAAC CGCATCTCCA ACGGCACTCT GGAGGAGGGG 
GTGACTTTAC CTGCTGGTTT TTGTGATGCG AGAAACGGGT CGTGTCTTTC TGATGATGTT 
GACGAGGATG ACGTGGAGCG CGCGTGTGAT TCTCTATGGA TGTGTATGAT CACTGTACTC 
AGTCATGGCC TCAGGAGCGG TGGAGGAGTT GGAGATGTCC TGCGCAAACC GTCTAAAGAG 
GAGCGTCTGT TCGCCGCACG GGTGGTGTAC GATCTCCTGT TCTTCTTCCT GGTGATCATC 
ATTGTGTTGA ATCTGATCTT CGGTGTGATC ATTGACACAT TTGCAGATCT GAGAAGCGAG 
AAGCAGAGAA AAGAGGAGAT CTTGAAGACC ACATGCTTCA TCTGTGGTCT GGAGAGAGAT 
AAGTTTGATA ATAAAACGGT GACGTTTGAA GAGCACATCA AGGAGGAGCA CAACCTGTGG 
CATTACCTCT TCTTCATAGT GCTGGTGCGG GTCAAAGACT CCACCGAATA CACGGGACCC 
GAGAGCTACG TCGCTCAGAT GATCAAGGAG CACAATCTGG ACTGGTTTCC TCGTATGAGG 
GCGATGTCTC TGGTCAGCAG TGATGGAGAA GGTGAACAGA ATGAACTGCG CAGCTTACAG 
GAGAAACTCG AGTCCACAAT GAGGCTCGTC TCTAATCTTA CCAATCAGCT CACAGAGCTC 
AAAGAGCAGA TGACTGAGCA AAGGAAACAC AAGCAGAGGC TCGGTCTGCT GGGAAACCCC 
GCCCACCTCA ACATCAACCC ACAGCAGCCA GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005166152.1
CDS	148..8283
Translation	MVDKLSSFLHIGDVCSLYAEGSTSGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGTSTTDTVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDAAGNEGSWFYIQPFYKLRSIGDNVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCTTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDEHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEVNPDYEEECLESRSSMDSEHEVIRARARNPQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSPLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAAKLEKGTITQNERRSVTKLLEDLVYFVVDIPSSAQDVLEITVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRYSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDPANADILIETKLVLSRFEVAGTVLGEGAEEEEEDEEEVWLFWKDSGGEVKSKSIRELAQDAKDGHTEDQEVINYYRYQLNLFARMCLDRQYLAINKISAQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSQISIDDYDNDGTSSDEVKERFSQTMEFVENYLRDVVCQSFPFSDKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHISTPFHIKLDREPGKGSNVMRSIHGVGELMTQVVLRGGGFLPASSHNPPDRDEVKAQSEPQKQDILVMDTKLKIIEILQFILNVRLDYRISCLLCIFKTEFDESNSQSEPSLSPESPASVQGALDFEHIEEQAEGIFGGSSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALHLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSDSGLDAGEVTPEAHHKGVINSSRSNKPKVESTSSSNYRVVKEILLRLSKLCVLEGISGKKNKKQQQRLLRNMGAHSVVLELLQIPYEKGEDVQMQEIMTLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAVTMQHIFMNNFQLCSEINERVVQHFVHCIETHGRSVHYLKFLQTIVKAENKFIKKCQDIVMAELVTAGEDVLVFYNDRASFQSLVQMMRLERERLDESSALRYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKMAYVNFLNHCYVDTEVEMKEIYTSNHMWKLFDDFLVDVCRVCNNTSDRKHADTVLERYVTETIMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQGVFRVYHCNWLLPGQKGNVEACIKVLSDVAKGRAIAIPVDLDCQVNNLFMKSNNIVQKTALSWRLSVRNATRRDSVLTTSRDYRNIIERLQDIVSALEERLRPLVQAELSVLVDVLHRPELLFPEHTEAHRKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKLLGYDDEMDVTEVVDISLPPKQQEDRQRGEALRQLLVNRYYGFRSGGRRESLTSFGNSTLTPVGPIKSQPGGLSRAEMSLMEVQCHLDREGASDLVIDLIMNTTSDRVFHESILLAIALLEGGNTIIQHSFFKRLTEDKNSEKFFRVFYDRMKVAQVEIKATVTVNTSDLGNKRRDDNTPDKDTPQRRRGKDSGVVVTDDAREQLLEASAATKKAFGSYRRDADPEETFGTADGDKGGGDKGTEQGEMSPVILIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINQHNVALINQTVESLTEYCQGPCHDNQNCIATHESNGIDIIIALILNDINPLGRKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVMKMAYQQGEAEFEDEEQENGEDHAASPRNVGHNIYILAHQLSRHNKELQILLKPGGEDQALEYYTKHTSQIEIVRQDRTMEQIVFPVPNICSFLTNESKLRVYYGTERDEQGSKINDFFLHADDLFNEMRWQKKLRAQPVLYWCSRNMSFWSNVSFNLAVLINLLVAFFYPLDGVSESQLEPSLSLLLWVCLLGSLGFVLMSPRPNAVRVLVISTVLQLGFSVGLQHMLTLLGAFNVCNKIVFMLSFVGNRGSFTRGYRVMVMDREFLFHLLYLLICILGLFGHVFFYSLLLFDLVNREETLLNVIKSVTRNGRSIVLTAVLGLILVYLFSIVGYMFFKDDFILEVNRISNGTLEEGVTLPAGFCDARNGSCLSDDVDEDDVERACDSLWMCMITVLSHGLRSGGGVGDVLRKPSKEERLFAARVVYDLLFFFLVIIIVLNLIFGVIIDTFADLRSEKQRKEEILKTTCFICGLERDKFDNKTVTFEEHIKEEHNLWHYLFFIVLVRVKDSTEYTGPESYVAQMIKEHNLDWFPRMRAMSLVSSDGEGEQNELRSLQEKLESTMRLVSNLTNQLTELKEQMTEQRKHKQRLGLLGNPAHLNINPQQPA

Target ORF information:

RefSeq Version	XM_005166095.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005166095.4

ORF Insert Sequence:

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GGTAAATCCA 
GACTATGAAG AAGAATGCCT GGAGTCCCGC TCCTCTATGG ATTCGGAGCA TGAAGTGATT 
CGGGCTCGTG CACGTAACCC TCAGGATAAG GTGATGTACA CACTGGTGTC TGTTCCTGAT 
GGAAATGACA TATCCTCCAT CTTTGAGCTT GACCCTACAA CTTTGAGGGG CGGAGACTCA 
CTTGTGCCAA GAAACTCGTA TGTGAGGCTC AGGCATCTCT GCACCAACAC GTGGGTCCAC 
AGCACCAACC AGCCAATAGA TAAAGAGGAA GAGAAACCGG TCATGCTCAG AATAGGAACG 
TCTCCTCTGA AGGAAGATAA AGAGGCGTTT GCTATAGTAC CAGTCTCTCC AGCTGAGGTT 
CGAGATCTGG ATTTTGCTAA TGATGCTAGT AAAGTGTTGG CTTCCATTGC TGCCAAACTG 
GAGAAGGGCA CCATTACCCA GAATGAGAGG AGGTCAGTGA CGAAGCTGTT GGAGGATCTG 
GTGTATTTTG TGGTGGACAT ACCCAGCAGT GCTCAAGATG TGCTGGAGAT CACAGTCAAC 
AAACCCAACA GAGAGCGACA GAAACTCATG AGAGAGCAGA ACATTCTTAA ACAGATCTTC 
AAGCTGCTGC AGGCGCCATT TACAGACAGC GGGGACGGCC CTATGTTGCG ATTAGAAGAG 
CTCGCAGACC AGCGTCATGC ACCCTTTCGA CACATTTGCA GACTCTGTTA CAGAGTCCTA 
CGTTACTCAC AACAGGACTA TCGCAAAAAT CAGGAGTACA TAGCAAAGCA GTTCCGCTTC 
ATGCAGAAGC AGATAGGATA TGATGTGCTG GCTGAAGACA CAATTACAGC CCTTCTGCAC 
AACAACAGGA AGCTCCTAGA GAAACACATC ACAGCAGCTG AAATAGATAC CTTTGTCAGT 
CTGGTCCGCA AGAACAGAGA GCCCAGATTT CTGGATTACT TGTCAGATCT GTGTGTGTCG 
ATGAATAAAT CCATCCCGGT CACTCAAGAG CTCATCTGTA ATGCTGTGCT GGACCCAGCC 
AATGCAGACA TTCTCATCGA AACCAAGCTT GTGTTGTCAC GGTTTGAGGT GGCAGGAACG 
GTGTTGGGTG AAGGTGCGGA GGAGGAAGAG GAGGATGAAG AGGAGGTCTG GCTGTTCTGG 
AAGGACAGTG GAGGAGAGGT GAAGAGTAAA AGCATACGAG AGCTTGCACA GGACGCAAAA 
GATGGACATA CAGAGGACCA GGAAGTCATC AATTATTACA GATATCAGCT GAATTTGTTT 
GCTCGGATGT GTCTGGATCG ACAGTATCTT GCGATTAACA AGATATCAGC ACAGCTGGAT 
GTGGATCTGA TTCTGCGCTG CATGTCAGAT GAAGATTTGC CCTTTGACCT CAGAGCATCG 
TTCTGCCGCA TGATGCTGCA CATGCATGTA GACCGTGACC CTCAGGAGCA GGTCACCCCG 
GTCAAATATG CTCGACTGTG GTCTGAAATC CCATCACAAA TTTCCATTGA TGATTATGAT 
AATGATGGGA CGTCAAGTGA TGAAGTGAAA GAGCGCTTCT CTCAGACGAT GGAGTTTGTG 
GAGAATTACC TGAGGGATGT TGTCTGCCAG AGTTTCCCGT TCTCTGATAA GGAGAAAAAC 
AAACTCACTT TTGAGGTGGT GAACCTGGCA AGAAACCTGA TCTATTTTGG TTTCTATAAC 
TTCAGTGATC TGCTAAGGTT AACAAAAATC CTATTGGCAA TCCTGGACTG TGTCCACATA 
AGCACTCCCT TCCACATCAA GCTGGATAGA GAACCCGGTA AAGGAAGTAA CGTGATGCGC 
TCCATCCATG GGGTGGGAGA GCTGATGACA CAGGTGGTGT TGAGGGGTGG GGGATTTCTG 
CCAGCCTCCA GCCACAATCC TCCTGACAGA GACGAAGTGA AAGCACAAAG TGAACCTCAG 
AAACAGGACA TACTGGTGAT GGACACTAAA CTCAAGATCA TCGAGATCCT GCAGTTTATT 
CTGAATGTGC GGTTGGACTA CAGGATCTCC TGCCTCCTCT GTATCTTTAA GACAGAGTTT 
GATGAAAGTA ACTCTCAAAG TGAACCATCT TTAAGCCCAG AATCTCCTGC CAGCGTTCAA 
GGTGCGCTAG ACTTTGAGCA CATTGAAGAG CAGGCGGAGG GTATTTTTGG AGGAAGTAGT 
GAAGAGAACA CCCCGCTGGA TCTTGATGAT CATGGTGGAC GGACATTTCT GAGGGTCCTG 
CTTCACCTGA CCATGCATGA CTATCCTCCG CTGGTATCTC GAGCTCTTCA CCTGCTCTTC 
AGACACTTTA GCCAACGCCA GGAGGTCCTA CAGGCCTTCA AACAGGTTCA GCTCCTGGTG 
ACCAGTCAGG ATGTGGAAAA CTATAAACAG ATCAAGTCAG ATTTGGACCA GCTGAGGTCA 
ATAGTAGAGA AATCAGAGTT GTGGGTGTAT AAGAGACAAG GCTCAGACTC AGGACTGGAT 
GCTGGAGAAG TCACCCCTGA AGCCCACCAC AAGGGTGTCA TCAACTCGAG TAGATCAAAC 
AAACCCAAAG TGGAAAGTAC CAGCAGCTCG AACTACAGAG TCGTGAAAGA GATCCTGCTG 
AGGCTGAGCA AACTGTGTGT TTTGGAGGGC ATCTCTGGCA AGAAGAATAA GAAACAGCAG 
CAGCGGCTCC TCAGGAACAT GGGAGCTCAC AGTGTGGTCC TCGAACTGTT GCAAATCCCT 
TATGAGAAGG GGGAGGATGT CCAGATGCAG GAGATCATGA CCTTAGCACA TCAGTTCCTT 
CAAAACTTCT GTGCTGGAAA TCAGCAGAAC CAGGCACTTC TACACAAACA CATCAACCTG 
TTCCTCAACC CTGGGATCCT GGAGGCGGTG ACCATGCAGC ATATCTTCAT GAATAATTTC 
CAGTTGTGTA GTGAGATTAA TGAGCGGGTC GTGCAGCATT TTGTGCACTG CATCGAGACA 
CACGGCCGCA GCGTGCACTA CCTCAAGTTC CTGCAGACCA TTGTCAAGGC AGAGAACAAG 
TTCATCAAGA AATGCCAAGA CATCGTCATG GCTGAGCTGG TGACTGCAGG TGAGGATGTG 
CTGGTGTTCT ATAATGACCG GGCGTCTTTT CAGTCATTGG TTCAGATGAT GCGTCTTGAG 
CGGGAACGAC TTGATGAGAG CAGTGCACTC AGGTATCACA TTCACCTGGT GGAGTTGCTG 
GCTGTGTGCA CTGAAGGCAA GAACGTCTAC ACTGAGATCA AATGCAACTC GCTGCTCCCT 
CTCGATGATA TTGTACGTGT GGTCACACAT GAGGACTGCA TCCCTGAGGT GAAGATGGCC 
TACGTGAACT TCCTGAATCA CTGTTATGTT GATACAGAGG TTGAGATGAA GGAGATCTAC 
ACCAGCAATC ACATGTGGAA ACTTTTTGAT GATTTTCTGG TGGACGTCTG CAGAGTGTGC 
AATAACACAA GTGACCGTAA ACACGCTGAC ACAGTTCTGG AGAGATACGT TACAGAGACC 
ATAATGAGCA TCGTCACCAC CTTTTTCAGC TCACCATTCT CAGACCAGAG CACAAGTTTG 
CAGACCCGGC AGCCGGTGTT TGTGCAGCTG CTGCAGGGAG TGTTTCGTGT ATATCACTGC 
AACTGGCTTC TCCCAGGTCA GAAGGGGAAT GTGGAAGCCT GCATCAAAGT GCTGAGTGAC 
GTGGCCAAAG GCAGAGCCAT AGCCATTCCT GTGGATCTGG ACTGTCAGGT GAATAATCTG 
TTCATGAAGT CCAACAACAT TGTGCAGAAA ACAGCCCTCA GCTGGAGACT CTCTGTCCGT 
AACGCGACTC GGAGAGACTC TGTTCTGACC ACCTCTCGAG ACTATCGCAA CATCATTGAA 
AGACTACAGG ATATAGTGTC TGCATTGGAG GAGCGGTTGA GGCCTCTGGT TCAGGCTGAG 
TTGTCAGTGT TGGTGGATGT TCTCCATCGC CCAGAGCTGC TGTTTCCAGA GCACACTGAA 
GCCCACCGCA AGTGTGAGAG TGGAGGCTTC ATCTGCAAGT TGATAAAACA CACTAAGCAG 
CTTCTAGAAG AAAACGAAGA GAGGCTTTGC ATCAAAGTCC TTCAGACACT CCGAGAGATG 
ATGACCAAAG ACAGAGGATA TGGAGAGAAG CTTCTGGGAT ATGATGATGA GATGGATGTC 
ACGGAGGTGG TGGATATCAG TCTGCCACCC AAACAGCAGG AGGATCGGCA GAGGGGGGAG 
GCCCTTCGGC AGCTGCTCGT CAATCGTTAC TATGGCTTCA GGAGTGGTGG GCGGAGAGAA 
AGTCTGACCT CGTTTGGTAA CTCCACCCTA ACTCCTGTCG GGCCAATCAA GAGCCAGCCA 
GGGGGGTTGA GCAGGGCTGA GATGTCATTA ATGGAGGTGC AGTGTCATCT AGACCGTGAA 
GGGGCATCGG ATCTTGTCAT TGATCTCATC ATGAACACTA CCAGCGACCG CGTCTTCCAT 
GAAAGCATTC TGTTGGCCAT CGCACTGTTG GAGGGCGGAA ACACCATCAT ACAGCATTCC 
TTCTTCAAAC GTTTAACAGA AGACAAAAAT TCAGAGAAGT TTTTCAGGGT GTTTTATGAC 
AGAATGAAAG TGGCTCAGGT GGAGATCAAA GCCACGGTTA CTGTCAACAC TAGTGACCTC 
GGCAACAAGC GGCGTGACGA CAACACTCCA GACAAAGATA CTCCACAACG CCGCAGAGGT 
AAAGACTCCG GTGTGGTGGT GACAGATGAT GCTCGTGAGC AGCTGTTGGA GGCTTCAGCT 
GCGACTAAGA AGGCCTTTGG TTCATATCGT CGAGATGCTG ATCCCGAGGA GACGTTTGGC 
ACGGCTGATG GAGATAAAGG AGGTGGAGAT AAAGGCACAG AGCAGGGCGA GATGAGCCCT 
GTCATCCTCA TAATGCAGCC AATCCTGCGC TTCCTGCAGC TGCTGTGCGA AAACCACAAT 
CGAGACCTGC AGAATTTTCT GCGCTGTCAG AACAATAAAA ACAACTATAA TCTGGTGTGT 
GAGACGCTGC AGTTCCTGGA CTGTATCTGT GGCAGCACAA CAGGAGGCCT GGGGCTGTTA 
GGACTCTACA TCAACCAGCA TAACGTAGCG CTCATTAATC AGACTGTAGA GAGCCTCACT 
GAATACTGTC AAGGTCCCTG CCATGACAAT CAGAATTGCA TTGCCACCCA TGAGTCTAAT 
GGCATCGACA TCATTATTGC ACTGATTCTG AATGATATTA ATCCACTGGG CAGGAAGAGA 
ATGGATCTGG TTCTAGAACT AAAGAATAAT GCATCTAAGC TGCTGCTGGC CATCATGGAG 
AGCCGTCATG ACAGTGAGAA TGCAGAAAGA ATTCTGTACA ACATGAGACC AAAAGAACTA 
GTGGAGGTGA TGAAAATGGC CTACCAGCAG GGGGAGGCAG AGTTTGAGGA TGAGGAACAG 
GAGAATGGAG AGGATCATGC AGCATCGCCT CGCAATGTTG GACACAACAT CTATATTCTT 
GCACATCAGC TGTCGCGTCA CAATAAGGAA TTGCAAATAC TACTGAAACC AGGTGGAGAG 
GACCAGGCCC TGGAGTATTA CACAAAACAC ACCTCACAGA TAGAGATAGT TCGACAGGAT 
CGCACTATGG AACAGATTGT GTTTCCTGTA CCAAATATCT GCTCTTTTCT GACAAATGAG 
TCCAAGCTGC GTGTGTACTA TGGTACAGAG AGAGACGAGC AAGGCAGTAA AATAAATGAC 
TTCTTCTTGC ATGCAGACGA CCTCTTTAAT GAAATGAGGT GGCAGAAAAA ACTAAGAGCT 
CAGCCAGTGC TGTACTGGTG CAGCAGGAAC ATGTCGTTTT GGAGTAACGT TTCCTTTAAT 
CTGGCGGTGC TCATTAATTT GCTGGTGGCA TTTTTCTACC CTCTGGATGG AGTGAGCGAA 
AGCCAGCTGG AACCTTCTCT GTCTCTGTTG CTGTGGGTTT GTTTGTTGGG CTCACTGGGC 
TTCGTGTTGA TGTCCCCGAG GCCAAATGCA GTGCGGGTGC TGGTCATCTC CACTGTCCTG 
CAGTTGGGCT TCTCTGTAGG GCTGCAGCAC ATGCTCACAC TCCTGGGAGC TTTCAATGTT 
TGTAATAAGA TCGTGTTCAT GCTGAGCTTT GTGGGTAACC GTGGGTCATT CACCCGGGGT 
TATCGGGTGA TGGTCATGGA TAGAGAGTTC CTGTTTCATC TGCTGTACCT TCTCATCTGC 
ATACTGGGCC TTTTTGGACA CGTCTTCTTT TACAGTCTTC TGTTGTTTGA CCTGGTGAAC 
CGGGAAGAGA CTCTTCTGAA CGTCATCAAG AGTGTGACTC GAAACGGCCG CTCCATCGTC 
TTGACCGCAG TGCTGGGCCT CATCCTGGTC TACCTCTTTT CTATTGTTGG ATATATGTTT 
TTCAAAGATG ACTTCATCCT GGAGGTGAAC CGCATCTCCA ACGGCACTCT GGAGGAGGGG 
GTGACTTTAC CTGCTGGTTT TTGTGATGCG AGAAACGGGT CGTGTCTTTC TGATGATGTT 
GACGAGGATG ACGTGGAGCG CGCGTGTGAT TCTCTATGGA TGTGTATGAT CACTGTACTC 
AGTCATGGCC TCAGGAGCGG TGGAGGAGTT GGAGATGTCC TGCGCAAACC GTCTAAAGAG 
GAGCGTCTGT TCGCCGCACG GGTGGTGTAC GATCTCCTGT TCTTCTTCCT GGTGATCATC 
ATTGTGTTGA ATCTGATCTT CGGTGTGATC ATTGACACAT TTGCAGATCT GAGAAGCGAG 
AAGCAGAGAA AAGAGGAGAT CTTGAAGACC ACATGCTTCA TCTGTGGTCT GGAGAGAGAT 
AAGTTTGATA ATAAAACGGT GACGTTTGAA GAGCACATCA AGGAGGAGCA CAACCTGTGG 
CATTACCTCT TCTTCATAGT GCTGGTGCGG GTCAAAGACT CCACCGAATA CACGGGACCC 
GAGAGCTACG TCGCTCAGAT GATCAAGGAG CACAATCTGG ACTGGTTTCC TCGTATGAGG 
GCGATGTCTC TGGTCAGCAG TGATGGAGAA GGTGAACAGA ATGAACTGCG CAGCTTACAG 
GAGAAACTCG AGTCCACAAT GAGGCTCGTC TCTAATCTTA CCAATCAGCT CACAGAGCTC 
AAAGAGCAGA TGACTGAGCA AAGGAAACAC AAGCAGAGGC TCGGTCTGCT GGGAAACCCC 
GCCCACCTCA ACATCAACCC ACAGCAGCCA GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itpr1a ( XM_021474612.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330287.1 Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X9, mRNA.

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CloneID	ODa146871
Clone ID Related Accession (Same CDS sequence)	XM_021474612.1
Accession Version	XM_021474612.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8169bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395382.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACAT TACAAGGTGC TGGAGTTTTT GCCATGGTGG ACAAATTGTC TAGTTTCCTG 
CACATTGGGG ACGTTTGTTC CCTTTATGCT GAAGGCTCTA CTAGTGGCTT CATCAGCACT 
CTGGGGTTGG TTGATGACCG ATGTGTTGTT CAACCAGATG CCGGAGACCT GAATAACCCA 
CCTAAAAAGT TTCGGGACTG TCTGTTCAGA CTGTGTCCCA TGAACAGATA TTCGGCACAG 
AAACAGTTCT GGAAAGCTGC CAAACCTGGA GGAACTAGCA CCACAGATAC TGTCCTGCTT 
AACAAACTCC ATCATGCTGC TGATTTAGAG AAGAAACAAA ATGATTCTGA GAACAAAAAG 
CTGCTTGGAA CCGTCATTCA GTATGGCAAT GTCATCCAGC TTCTCCATCT GAAGAGTAAT 
AAATATCTGA CGGTGAATAA GCGTTTGCCG GCTCTTCTTG AGAAGAATGC CATGCGTGTG 
ACTCTGGACG CTGCAGGGAA TGAAGGCTCG TGGTTTTACA TCCAGCCCTT CTATAAGCTG 
CGCTCTATAG GGGATAATGT GGTCATTGGA GATAAGGTCG TGTTAAACCC TGTCAATGCC 
GGACAGCCGC TGCACGCCAG CAGCCATCAG CTTGTGGATA ATCCAGGCTG CAATGAAGTG 
AATTCAGTGA ACTGCACCAC CAGTTGGAAA ATCGTTCTCT TCATGAAATG GAGCGACAAC 
CAGGAGATTG TCTTGAAAGG AGGTGACGTG GTGAGGCTGT TTCATGCAGA GCAGGAGAAG 
TTTCTGACAT GTGATGAGCA TCGGAAAAAG CAGTATGTGT TTCTGCGTAC CACCGGACGC 
CAGTCTGCAA CCTCCGCCAC TAGTAGCAAA GCACTCTGGG AGGTAGAGGT GGTTCAGCAT 
GATCCCTGCC GAGGCGGAGC CGGCTACTGG AACAGCCTCT TTAGGTTTAA ACACTTAGCC 
ACCGGCCATT ACCTTGCTGC AGAGGTAAAT CCAGACTATG AAGAAGAATG CCTGGAGTCC 
CGCTCCTCTA TGGATTCGGA GCATGAAGTG ATTCGGGCTC GTGCACGTAA CCCTCAGGAT 
AAGGTGATGT ACACACTGGT GTCTGTTCCT GATGGAAATG ACATATCCTC CATCTTTGAG 
CTTGACCCTA CAACTTTGAG GGGCGGAGAC TCACTTGTGC CAAGAAACTC GTATGTGAGG 
CTCAGGCATC TCTGCACCAA CACGTGGGTC CACAGCACCA ACCAGCCAAT AGATAAAGAG 
GAAGAGAAAC CGGTCATGCT CAGAATAGGA ACGTCTCCTC TGAAGGAAGA TAAAGAGGCG 
TTTGCTATAG TACCAGTCTC TCCAGCTGAG GTTCGAGATC TGGATTTTGC TAATGATGCT 
AGTAAAGTGT TGGCCTCCAT TGCTGCCAAA CTGGAGAAGG GCACCATCAC CCAGAATGAG 
AGGAGGTCAG TGACGAAGCT GTTGGAGGAT CTGGTGTATT TTGTGGTGGA CATACCCAGC 
AGTGCTCAAG ATGTGCTGGA GATCACAGTC AACAAACCCA ACAGAGAGCG ACAGAAACTC 
ATGAGAGAGC AGAACATTCT TAAACAGATC TTCAAGCTGC TGCAGGCGCC ATTTACAGAC 
AGCGGGGACG GCCCTATGTT GCGATTAGAA GAGCTCGCAG ACCAGCGTCA TGCACCCTTT 
CGACACATTT GCAGACTCTG TTACAGAGTC CTACGTTACT CACAACAGGA CTATCGCAAA 
AATCAGGAGT ACATAGCAAA GCAGTTCCGC TTCATGCAGA AGCAGATAGG ATATGATGTG 
CTGGCTGAAG ACACAATTAC AGCCCTTCTG CACAACAACA GGAAGCTCCT AGAGAAACAC 
ATCACAGCAG CTGAAATAGA TACCTTTGTC AGTCTGGTCC GCAAGAACAG AGAGCCCAGA 
TTTCTGGATT ACTTGTCAGA TCTGTGTGTG TCGATGAATA AATCCATCCC GGTCACTCAA 
GAGCTCATCT GTAATGCTGT GCTGGACCCA GCCAATGCAG ACATTCTCAT CGAAACCAAG 
CTTGTGTTGT CACGATTTGA GGTGGCAGGA ACGGTGTTGG GTGAAGGTGC GGAGGAGGAA 
GAGGAGGATG AAGAGGAGGT CTGGCTGTTC TGGAAGGACA GTGGAGGAGA GGTGAAGAGT 
AAAAGCATAC GAGAGCTTGC ACAGGACGCA AAAGATGGAC ATACAGAGGA CCAGGAAGTC 
ATCAATTATT ACAGATATCA GCTGAATTTG TTTGCTCGGA TGTGTCTGGA TCGACAGTAT 
CTTGCGATTA ACAAGATATC AGCACAGCTG GATGTGGATC TGATTCTGCG CTGCATGTCA 
GATGAAGATT TGCCCTTTGA CCTCAGAGCA TCGTTCTGCC GCATGATGCT GCACATGCAT 
GTAGACCGTG ACCCTCAGGA GCAGGTCACC CCGGTCAAAT ATGCTCGACT GTGGTCTGAA 
ATCCCATCAC AAATTTCCAT TGATGATTAT GATAATGATG GGACGTCACG TGATGAAGTG 
AAAGAGCGCT TCTCTCAGAC GATGGAGTTT GTGGAGAATT ACCTGAGGGA TGTTGTCTGC 
CAGAGTTTCC CGTTCTCTGA TAAGGAGAAA AACAAACTCA CTTTTGAGGT GGTGAACCTG 
GCAAGAAACC TGATCTATTT TGGTTTCTAT AACTTCAGTG ATCTGCTAAG GTTAACAAAA 
ATCCTATTGG CAATCCTGGA CTGTGTCCAC ATAAGCACTC CCTTCCACAT CAAGCTGGAT 
AGAGAACCTG GTAAAGGAAG TAACGTGATG CGCTCCATCC ATGGGGTGGG AGAACTGATG 
ACACAGGTAG TGTTGAGGGG TGGGGGATTT CTGCCAGCCT CCAGCCACAA TCCTCCTGAC 
AGAGACGAAG TGAAAGCACA AAGTGAACCT CAGAAACAAG ACATACTGGT GATGGACACT 
AAACTCAAGA TCATCGAGAT CCTGCAGTTT ATTCTGAATG TGCGGTTGGA CTACAGGATC 
TCCTGCCTCC TCTGTATCTT TAAGACAGAG TTTGATGAAA GTAACTCTCA AAGTGAACCA 
TCTTTAAGCC CAGAATCTCC TGCCAGCGTT CAAGGTGCGC TAGACTTTGA GCACATTGAA 
GAGCAGGCGG AGGGTATTTT TGGAGGAAGT AGTGAAGAGA ACACCCCGCT GGATCTTGAT 
GATCATGGTG GACGGACATT TCTGAGGGTC CTGCTTCACC TGACCATGCA TGACTATCCT 
CCGCTGGTAT CTCGAGCTCT TCACCTGCTC TTCAGACACT TTAGCCAACG CCAGGAGGTC 
CTACAGGCCT TCAAACAGGT TCAGCTCCTG GTGACCAGTC AGGATGTGGA AAACTATAAA 
CAGATCAAGT CAGATTTGGA CCAGCTGAGG TCAATAGTAG AGAAATCAGA GTTGTGGGTG 
TATAAGAGAC AAGGCTCAGA CTCAGGACTG GATGCTGGAG AAGTCACCCC TGAAGCCCAC 
CACAAGGGTG TCATCAACTC GAGTAGATCA AACAAACCCA AAGTGGAAAG TACCAGCAGC 
TCGAACTACA GAGTCGTGAA AGAGATCCTG CTGAGGCTGA GCAAACTGTG TGTTTTGGAG 
GGCATCTCTG GCAAGAAGAA TAAGAAACAG CAGCAGCGGC TCCTCAGGAA CATGGGAGCT 
CACAGTGTGG TCCTCGAACT GTTGCAAATC CCTTATGAGA AGGGGGAGGA TGTCCAGATG 
CAGGAGATCA TGACCTTAGC ACATCAGTTC CTTCAAAACT TCTGTGCTGG AAATCAGCAG 
AACCAGGCAC TTCTACACAA ACACATCAAC CTGTTCCTCA ACCCTGGGAT CCTGGAGGCG 
GTGACCATGC AGCATATCTT CATGAATAAT TTCCAGTTGT GTAGTGAGAT TAATGAGCGG 
GTCGTGCAGC ATTTTGTGCA CTGCATCGAG ACACACGGCC GCAGCGTGCA CTACCTCAAG 
TTCCTGCAGA CCATTGTCAA GGCAGAGAAC AAGTTCATCA AGAAATGCCA AGACATCGTC 
ATGGCTGAGC TGGTGACTGC AGGTGAGGAT GTGCTGGTGT TCTATAATGA CCGGGCGTCT 
TTTCAGTCAT TGGTTCAGAT GATGCGTCTT GAGCGGGAAC GACTTGATGA GAGCAGTGCA 
CTCAGGTATC ACATTCACCT GGTGGAGTTG CTGGCTGTGT GCACTGAAGG CAAGAACGTC 
TACACTGAGA TCAAATGCAA CTCGCTGCTC CCTCTCGATG ATATTGTACG TGTGGTCACA 
CATGAGGACT GCATCCCTGA GGTGAAGATG GCCTACGTGA ACTTCCTGAA TCACTGTTAT 
GTTGATACAG AGGTTGAGAT GAAGGAGATC TACACCAGCA ATCACATGTG GAAACTTTTT 
GATGATTTTC TGGTGGACGT CTGCAGAGTG TGCAATAACA CAAGTGACCG TAAACACGCT 
GACACAGTTC TGGAGAGATA CGTTACAGAG ACCATAATGA GCATCGTCAC CACCTTTTTC 
AGCTCACCAT TCTCAGACCA GAGCACAAGT TTGCAGACCC GGCAGCCGGT GTTTGTGCAG 
CTGCTGCAGG GAGTGTTTCG TGTATATCAC TGCAACTGGC TTCTCCCAGG TCAGAAGGGG 
AATGTGGAAG CCTGCATCAA AGTGCTGAGT GACGTGGCCA AAGGCAGAGC CATAGCCATT 
CCTGTGGATC TGGACTGTCA GGTGAATAAT CTGTTCATGA AGTCCAACAA CATTGTGCAG 
AAAACAGCCC TCAGCTGGAG ACTCTCTGTC CGTAACGCGA CTCGGAGAGA CTCTGTTCTG 
ACCACCTCTC GAGACTATCG CAACATCATT GAAAGACTAC AGGATATAGT GTCTGCATTG 
GAGGAGCGGT TGAGGCCTCT GGTTCAGGCT GAGTTGTCAG TGTTGGTGGA TGTTCTCCAT 
CGCCCAGAGC TGCTGTTTCC AGAGCACACT GAAGCCCACC GCAAGTGTGA GAGTGGAGGC 
TTCATCTGCA AGTTGATAAA ACACACTAAG CAGCTTCTAG AAGAAAACGA AGAGAGGCTT 
TGCATCAAAG TCCTTCAGAC ACTCCGAGAG ATGATGACCA AAGACAGAGG ATATGGAGAG 
AAGCTTCTGG GATATGATGA TGAGATGGAT GTCACGGAGG TGGTGGATAT CAGTCTGCCA 
CCCAAACAGC AGGAGGATCG GCAGAGGGGG GAGGCCCTTC GGCAGCTGCT CGTCAATCGT 
TACTATGGCT TCAGGAGTGG TGGGCGGAGA GAAAGTCTGA CCTCGTTTGG TAACTCCACC 
CTAACTCCTG TCGGGCCAAT CAAGAGCCAG CCAGGGGGGT TGAGCAGGGC TGAGATGTCA 
TTAATGGAGG TGCAGTGTCA TCTAGACCGT GAAGGGGCAT CGGATCTTGT CATTGATCTC 
ATCATGAACA CTACCAGCGA CCGCGTCTTC CATGAAAGCA TTCTGTTGGC CATCGCACTG 
TTGGAGGGCG GAAACACCAT CATACAGCAT TCCTTCTTCA AACGTTTAAC AGAAGACAAA 
AATTCAGAGA AGTTTTTCAG GGTGTTTTAT GACAGAATGA AAGTGGCTCA GGTGGAGATC 
AAAGCCACGG TTACTGTCAA CACTAGTGAC CTCGGCAACA AGCGGCGTGA CGACAACACT 
CCAGACAAAG ATACTCCACA ACGCCGCAGA GGTAAAGACT CCGGTGTGGT GGTGACAGAT 
GATGCTCGTG AGCAGCTGTT GGAGGCTTCA GCTGCGACTA AGAAGGCCTT TGGTTCATAT 
CGTCGAGATG CTGATCCCGA GGAGACGTTT GGCACGGCTG ATGGAGATAA AGGAGGTGGA 
GATAAAGGCA CAGAGCAGGG CGAGATGAGC CCTGTCATCC TCATAATGCA GCCAATCCTG 
CGCTTCCTGC AGCTGCTGTG CGAAAACCAC AATCGAGACC TGCAGAATTT TCTGCGCTGT 
CAGAACAATA AAAACAACTA TAATCTGGTG TGTGAGACGC TGCAGTTCCT GGACTGTATC 
TGTGGCAGCA CAACAGGAGG CCTGGGGCTG TTAGGACTCT ACATCAACCA GCATAACGTA 
GCGCTCATTA ATCAGACTGT AGAGAGCCTC ACTGAATACT GTCAAGGTCC CTGCCATGAC 
AATCAGAATT GCATTGCCAC CCATGAGTCT AATGGCATCG ACATCATTAT TGCACTGATT 
CTGAATGATA TTAATCCACT GGGCAGGAAG AGAATGGATC TGGTTCTAGA ACTAAAGAAT 
AATGCATCTA AGCTGCTGCT GGCCATCATG GAGAGCCGTC ATGACAGTGA GAATGCAGAA 
AGAATTCTGT ACAACATGAG ACCAAAAGAA CTAGTGGAGG TGATGAAAAT GGCCTACCAG 
CAGGGGGAGG CAGAGTTTGA GGATGAGGAA CAGGAGAATG GAGAGGATCA TGCAGCATCG 
CCTCGCAATG TTGGACACAA CATCTATATT CTTGCACATC AGCTGTCGCG TCACAATAAG 
GAATTGCAAA TACTACTGAA ACCAGGTGGA GAGGACCAGG CCCTGGAGTA TTACACAAAA 
CACACCTCAC AGATAGAGAT AGTTCGACAG GATCGCACTA TGGAACAGAT TGTGTTTCCT 
GTACCAAATA TCTGCTCTTT TCTGACAAAT GAGTCCAAGC TGCGTGTGTA CTATGGTACA 
GAGAGAGACG AGCAAGGCAG TAAAATAAAT GACTTCTTCT TGCATGCAGA CGACCTCTTT 
AATGAAATGA GGTGGCAGAA AAAACTAAGA GCTCAGCCAG TGCTGTACTG GTGCAGCAGG 
AACATGTCGT TTTGGAGTAA CGTTTCCTTT AATCTGGCGG TGCTCATTAA TTTGCTGGTG 
GCATTTTTCT ACCCTCTGGA TGGAGTGAGC GAAAGCCAGC TGGAACCTTC TCTGTCTCTG 
TTGCTGTGGG TTTGTTTGTT GGGCTCACTG GGCTTCGTGT TGATGTCCCC GAGGCCAAAT 
GCAGTGCGGG TGCTGGTCAT CTCCACTGTC CTGCAGTTGG GCTTCTCTGT AGGGCTGCAG 
CACATGCTCA CACTCCTGGG AGCTTTCAAT GTTTGTAATA AGATCGTGTT CATGCTGAGC 
TTTGTGGGTA ACCGTGGGTC ATTCACCCGG GGTTATCGGG TGATGGTCAT GGATAGAGAG 
TTCCTGTTTC ATCTGCTGTA CCTTCTCATC TGCATACTGG GCCTTTTTGG ACACGTCTTC 
TTTTACAGTC TTCTGTTGTT TGACCTGGTG AACCGGGAAG AGACTCTTCT GAACGTCATC 
AAGAGTGTGA CTCGAAACGG CCGCTCCATC GTCTTGACCG CAGTGCTGGG CCTCATCCTG 
GTCTACCTCT TTTCTATTGT TGGATATATG TTTTTCAAAG ATGACTTCAT CCTGGAGGTG 
AACCGCATCT CCAACGGCAC TCTGGAGGAG GGGGTGACTT TACCTGCTGG TTTTTGTGAT 
GCGAGAAACG GGTCGTGTCT TTCTGATGAT GTTGACGAGG ATGACGTGGA GCGCGCGTGT 
GATTCTCTAT GGATGTGTAT GATCACTGTA CTCAGTCATG GCCTCAGGAG CGGTGGAGGA 
GTTGGAGATG TCCTGCGCAA ACCGTCTAAA GAGGAGCGTC TGTTCGCCGC ACGGGTGGTG 
TACGATCTCC TGTTCTTCTT CCTGGTGATC ATCATTGTGT TGAATCTGAT CTTCGGTGTG 
ATCATTGACA CATTTGCAGA TCTGAGAAGC GAGAAGCAGA GAAAAGAGGA GATCTTGAAG 
ACCACATGCT TCATCTGTGG TCTGGAGAGA GATAAGTTTG ATAATAAAAC GGTGACGTTT 
GAAGAGCACA TCAAGGAGGA GCACAACCTG TGGCATTACC TCTTCTTCAT AGTGCTGGTG 
CGGGTCAAAG ACTCCACCGA ATACACGGGA CCCGAGAGCT ACGTCGCTCA GATGATCAAG 
GAGCACAATC TGGACTGGTT TCCTCGTATG AGGGCGATGT CTCTGGTCAG CAGTGATGGA 
GAAGGTGAAC AGAATGAACT GCGCAGCTTA CAGGAGAAAC TCGAGTCCAC AATGAGGCTC 
GTCTCTAATC TTACCAATCA GCTCACAGAG CTCAAAGAGC AGATGACTGA GCAAAGGAAA 
CACAAGCAGA GGCTCGGTCT GCTGGGAAAC CCCGCCCACC TCAACATCAA CCCACAGCAG 
CCAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330287.1
CDS	265..8433
Translation	MMTLQGAGVFAMVDKLSSFLHIGDVCSLYAEGSTSGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGTSTTDTVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDAAGNEGSWFYIQPFYKLRSIGDNVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCTTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDEHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEVNPDYEEECLESRSSMDSEHEVIRARARNPQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSPLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAAKLEKGTITQNERRSVTKLLEDLVYFVVDIPSSAQDVLEITVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRYSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDPANADILIETKLVLSRFEVAGTVLGEGAEEEEEDEEEVWLFWKDSGGEVKSKSIRELAQDAKDGHTEDQEVINYYRYQLNLFARMCLDRQYLAINKISAQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSQISIDDYDNDGTSRDEVKERFSQTMEFVENYLRDVVCQSFPFSDKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHISTPFHIKLDREPGKGSNVMRSIHGVGELMTQVVLRGGGFLPASSHNPPDRDEVKAQSEPQKQDILVMDTKLKIIEILQFILNVRLDYRISCLLCIFKTEFDESNSQSEPSLSPESPASVQGALDFEHIEEQAEGIFGGSSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALHLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSDSGLDAGEVTPEAHHKGVINSSRSNKPKVESTSSSNYRVVKEILLRLSKLCVLEGISGKKNKKQQQRLLRNMGAHSVVLELLQIPYEKGEDVQMQEIMTLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAVTMQHIFMNNFQLCSEINERVVQHFVHCIETHGRSVHYLKFLQTIVKAENKFIKKCQDIVMAELVTAGEDVLVFYNDRASFQSLVQMMRLERERLDESSALRYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKMAYVNFLNHCYVDTEVEMKEIYTSNHMWKLFDDFLVDVCRVCNNTSDRKHADTVLERYVTETIMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQGVFRVYHCNWLLPGQKGNVEACIKVLSDVAKGRAIAIPVDLDCQVNNLFMKSNNIVQKTALSWRLSVRNATRRDSVLTTSRDYRNIIERLQDIVSALEERLRPLVQAELSVLVDVLHRPELLFPEHTEAHRKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKLLGYDDEMDVTEVVDISLPPKQQEDRQRGEALRQLLVNRYYGFRSGGRRESLTSFGNSTLTPVGPIKSQPGGLSRAEMSLMEVQCHLDREGASDLVIDLIMNTTSDRVFHESILLAIALLEGGNTIIQHSFFKRLTEDKNSEKFFRVFYDRMKVAQVEIKATVTVNTSDLGNKRRDDNTPDKDTPQRRRGKDSGVVVTDDAREQLLEASAATKKAFGSYRRDADPEETFGTADGDKGGGDKGTEQGEMSPVILIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINQHNVALINQTVESLTEYCQGPCHDNQNCIATHESNGIDIIIALILNDINPLGRKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVMKMAYQQGEAEFEDEEQENGEDHAASPRNVGHNIYILAHQLSRHNKELQILLKPGGEDQALEYYTKHTSQIEIVRQDRTMEQIVFPVPNICSFLTNESKLRVYYGTERDEQGSKINDFFLHADDLFNEMRWQKKLRAQPVLYWCSRNMSFWSNVSFNLAVLINLLVAFFYPLDGVSESQLEPSLSLLLWVCLLGSLGFVLMSPRPNAVRVLVISTVLQLGFSVGLQHMLTLLGAFNVCNKIVFMLSFVGNRGSFTRGYRVMVMDREFLFHLLYLLICILGLFGHVFFYSLLLFDLVNREETLLNVIKSVTRNGRSIVLTAVLGLILVYLFSIVGYMFFKDDFILEVNRISNGTLEEGVTLPAGFCDARNGSCLSDDVDEDDVERACDSLWMCMITVLSHGLRSGGGVGDVLRKPSKEERLFAARVVYDLLFFFLVIIIVLNLIFGVIIDTFADLRSEKQRKEEILKTTCFICGLERDKFDNKTVTFEEHIKEEHNLWHYLFFIVLVRVKDSTEYTGPESYVAQMIKEHNLDWFPRMRAMSLVSSDGEGEQNELRSLQEKLESTMRLVSNLTNQLTELKEQMTEQRKHKQRLGLLGNPAHLNINPQQPA

Target ORF information:

RefSeq Version	XM_021474612.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474612.1

ORF Insert Sequence:

ATGATGACAT TACAAGGTGC TGGAGTTTTT GCCATGGTGG ACAAATTGTC TAGTTTCCTG 
CACATTGGGG ACGTTTGTTC CCTTTATGCT GAAGGCTCTA CTAGTGGCTT CATCAGCACT 
CTGGGGTTGG TTGATGACCG ATGTGTTGTT CAACCAGATG CCGGAGACCT GAATAACCCA 
CCTAAAAAGT TTCGGGACTG TCTGTTCAGA CTGTGTCCCA TGAACAGATA TTCGGCACAG 
AAACAGTTCT GGAAAGCTGC CAAACCTGGA GGAACTAGCA CCACAGATAC TGTCCTGCTT 
AACAAACTCC ATCATGCTGC TGATTTAGAG AAGAAACAAA ATGATTCTGA GAACAAAAAG 
CTGCTTGGAA CCGTCATTCA GTATGGCAAT GTCATCCAGC TTCTCCATCT GAAGAGTAAT 
AAATATCTGA CGGTGAATAA GCGTTTGCCG GCTCTTCTTG AGAAGAATGC CATGCGTGTG 
ACTCTGGACG CTGCAGGGAA TGAAGGCTCG TGGTTTTACA TCCAGCCCTT CTATAAGCTG 
CGCTCTATAG GGGATAATGT GGTCATTGGA GATAAGGTCG TGTTAAACCC TGTCAATGCC 
GGACAGCCGC TGCACGCCAG CAGCCATCAG CTTGTGGATA ATCCAGGCTG CAATGAAGTG 
AATTCAGTGA ACTGCACCAC CAGTTGGAAA ATCGTTCTCT TCATGAAATG GAGCGACAAC 
CAGGAGATTG TCTTGAAAGG AGGTGACGTG GTGAGGCTGT TTCATGCAGA GCAGGAGAAG 
TTTCTGACAT GTGATGAGCA TCGGAAAAAG CAGTATGTGT TTCTGCGTAC CACCGGACGC 
CAGTCTGCAA CCTCCGCCAC TAGTAGCAAA GCACTCTGGG AGGTAGAGGT GGTTCAGCAT 
GATCCCTGCC GAGGCGGAGC CGGCTACTGG AACAGCCTCT TTAGGTTTAA ACACTTAGCC 
ACCGGCCATT ACCTTGCTGC AGAGGTAAAT CCAGACTATG AAGAAGAATG CCTGGAGTCC 
CGCTCCTCTA TGGATTCGGA GCATGAAGTG ATTCGGGCTC GTGCACGTAA CCCTCAGGAT 
AAGGTGATGT ACACACTGGT GTCTGTTCCT GATGGAAATG ACATATCCTC CATCTTTGAG 
CTTGACCCTA CAACTTTGAG GGGCGGAGAC TCACTTGTGC CAAGAAACTC GTATGTGAGG 
CTCAGGCATC TCTGCACCAA CACGTGGGTC CACAGCACCA ACCAGCCAAT AGATAAAGAG 
GAAGAGAAAC CGGTCATGCT CAGAATAGGA ACGTCTCCTC TGAAGGAAGA TAAAGAGGCG 
TTTGCTATAG TACCAGTCTC TCCAGCTGAG GTTCGAGATC TGGATTTTGC TAATGATGCT 
AGTAAAGTGT TGGCCTCCAT TGCTGCCAAA CTGGAGAAGG GCACCATCAC CCAGAATGAG 
AGGAGGTCAG TGACGAAGCT GTTGGAGGAT CTGGTGTATT TTGTGGTGGA CATACCCAGC 
AGTGCTCAAG ATGTGCTGGA GATCACAGTC AACAAACCCA ACAGAGAGCG ACAGAAACTC 
ATGAGAGAGC AGAACATTCT TAAACAGATC TTCAAGCTGC TGCAGGCGCC ATTTACAGAC 
AGCGGGGACG GCCCTATGTT GCGATTAGAA GAGCTCGCAG ACCAGCGTCA TGCACCCTTT 
CGACACATTT GCAGACTCTG TTACAGAGTC CTACGTTACT CACAACAGGA CTATCGCAAA 
AATCAGGAGT ACATAGCAAA GCAGTTCCGC TTCATGCAGA AGCAGATAGG ATATGATGTG 
CTGGCTGAAG ACACAATTAC AGCCCTTCTG CACAACAACA GGAAGCTCCT AGAGAAACAC 
ATCACAGCAG CTGAAATAGA TACCTTTGTC AGTCTGGTCC GCAAGAACAG AGAGCCCAGA 
TTTCTGGATT ACTTGTCAGA TCTGTGTGTG TCGATGAATA AATCCATCCC GGTCACTCAA 
GAGCTCATCT GTAATGCTGT GCTGGACCCA GCCAATGCAG ACATTCTCAT CGAAACCAAG 
CTTGTGTTGT CACGATTTGA GGTGGCAGGA ACGGTGTTGG GTGAAGGTGC GGAGGAGGAA 
GAGGAGGATG AAGAGGAGGT CTGGCTGTTC TGGAAGGACA GTGGAGGAGA GGTGAAGAGT 
AAAAGCATAC GAGAGCTTGC ACAGGACGCA AAAGATGGAC ATACAGAGGA CCAGGAAGTC 
ATCAATTATT ACAGATATCA GCTGAATTTG TTTGCTCGGA TGTGTCTGGA TCGACAGTAT 
CTTGCGATTA ACAAGATATC AGCACAGCTG GATGTGGATC TGATTCTGCG CTGCATGTCA 
GATGAAGATT TGCCCTTTGA CCTCAGAGCA TCGTTCTGCC GCATGATGCT GCACATGCAT 
GTAGACCGTG ACCCTCAGGA GCAGGTCACC CCGGTCAAAT ATGCTCGACT GTGGTCTGAA 
ATCCCATCAC AAATTTCCAT TGATGATTAT GATAATGATG GGACGTCACG TGATGAAGTG 
AAAGAGCGCT TCTCTCAGAC GATGGAGTTT GTGGAGAATT ACCTGAGGGA TGTTGTCTGC 
CAGAGTTTCC CGTTCTCTGA TAAGGAGAAA AACAAACTCA CTTTTGAGGT GGTGAACCTG 
GCAAGAAACC TGATCTATTT TGGTTTCTAT AACTTCAGTG ATCTGCTAAG GTTAACAAAA 
ATCCTATTGG CAATCCTGGA CTGTGTCCAC ATAAGCACTC CCTTCCACAT CAAGCTGGAT 
AGAGAACCTG GTAAAGGAAG TAACGTGATG CGCTCCATCC ATGGGGTGGG AGAACTGATG 
ACACAGGTAG TGTTGAGGGG TGGGGGATTT CTGCCAGCCT CCAGCCACAA TCCTCCTGAC 
AGAGACGAAG TGAAAGCACA AAGTGAACCT CAGAAACAAG ACATACTGGT GATGGACACT 
AAACTCAAGA TCATCGAGAT CCTGCAGTTT ATTCTGAATG TGCGGTTGGA CTACAGGATC 
TCCTGCCTCC TCTGTATCTT TAAGACAGAG TTTGATGAAA GTAACTCTCA AAGTGAACCA 
TCTTTAAGCC CAGAATCTCC TGCCAGCGTT CAAGGTGCGC TAGACTTTGA GCACATTGAA 
GAGCAGGCGG AGGGTATTTT TGGAGGAAGT AGTGAAGAGA ACACCCCGCT GGATCTTGAT 
GATCATGGTG GACGGACATT TCTGAGGGTC CTGCTTCACC TGACCATGCA TGACTATCCT 
CCGCTGGTAT CTCGAGCTCT TCACCTGCTC TTCAGACACT TTAGCCAACG CCAGGAGGTC 
CTACAGGCCT TCAAACAGGT TCAGCTCCTG GTGACCAGTC AGGATGTGGA AAACTATAAA 
CAGATCAAGT CAGATTTGGA CCAGCTGAGG TCAATAGTAG AGAAATCAGA GTTGTGGGTG 
TATAAGAGAC AAGGCTCAGA CTCAGGACTG GATGCTGGAG AAGTCACCCC TGAAGCCCAC 
CACAAGGGTG TCATCAACTC GAGTAGATCA AACAAACCCA AAGTGGAAAG TACCAGCAGC 
TCGAACTACA GAGTCGTGAA AGAGATCCTG CTGAGGCTGA GCAAACTGTG TGTTTTGGAG 
GGCATCTCTG GCAAGAAGAA TAAGAAACAG CAGCAGCGGC TCCTCAGGAA CATGGGAGCT 
CACAGTGTGG TCCTCGAACT GTTGCAAATC CCTTATGAGA AGGGGGAGGA TGTCCAGATG 
CAGGAGATCA TGACCTTAGC ACATCAGTTC CTTCAAAACT TCTGTGCTGG AAATCAGCAG 
AACCAGGCAC TTCTACACAA ACACATCAAC CTGTTCCTCA ACCCTGGGAT CCTGGAGGCG 
GTGACCATGC AGCATATCTT CATGAATAAT TTCCAGTTGT GTAGTGAGAT TAATGAGCGG 
GTCGTGCAGC ATTTTGTGCA CTGCATCGAG ACACACGGCC GCAGCGTGCA CTACCTCAAG 
TTCCTGCAGA CCATTGTCAA GGCAGAGAAC AAGTTCATCA AGAAATGCCA AGACATCGTC 
ATGGCTGAGC TGGTGACTGC AGGTGAGGAT GTGCTGGTGT TCTATAATGA CCGGGCGTCT 
TTTCAGTCAT TGGTTCAGAT GATGCGTCTT GAGCGGGAAC GACTTGATGA GAGCAGTGCA 
CTCAGGTATC ACATTCACCT GGTGGAGTTG CTGGCTGTGT GCACTGAAGG CAAGAACGTC 
TACACTGAGA TCAAATGCAA CTCGCTGCTC CCTCTCGATG ATATTGTACG TGTGGTCACA 
CATGAGGACT GCATCCCTGA GGTGAAGATG GCCTACGTGA ACTTCCTGAA TCACTGTTAT 
GTTGATACAG AGGTTGAGAT GAAGGAGATC TACACCAGCA ATCACATGTG GAAACTTTTT 
GATGATTTTC TGGTGGACGT CTGCAGAGTG TGCAATAACA CAAGTGACCG TAAACACGCT 
GACACAGTTC TGGAGAGATA CGTTACAGAG ACCATAATGA GCATCGTCAC CACCTTTTTC 
AGCTCACCAT TCTCAGACCA GAGCACAAGT TTGCAGACCC GGCAGCCGGT GTTTGTGCAG 
CTGCTGCAGG GAGTGTTTCG TGTATATCAC TGCAACTGGC TTCTCCCAGG TCAGAAGGGG 
AATGTGGAAG CCTGCATCAA AGTGCTGAGT GACGTGGCCA AAGGCAGAGC CATAGCCATT 
CCTGTGGATC TGGACTGTCA GGTGAATAAT CTGTTCATGA AGTCCAACAA CATTGTGCAG 
AAAACAGCCC TCAGCTGGAG ACTCTCTGTC CGTAACGCGA CTCGGAGAGA CTCTGTTCTG 
ACCACCTCTC GAGACTATCG CAACATCATT GAAAGACTAC AGGATATAGT GTCTGCATTG 
GAGGAGCGGT TGAGGCCTCT GGTTCAGGCT GAGTTGTCAG TGTTGGTGGA TGTTCTCCAT 
CGCCCAGAGC TGCTGTTTCC AGAGCACACT GAAGCCCACC GCAAGTGTGA GAGTGGAGGC 
TTCATCTGCA AGTTGATAAA ACACACTAAG CAGCTTCTAG AAGAAAACGA AGAGAGGCTT 
TGCATCAAAG TCCTTCAGAC ACTCCGAGAG ATGATGACCA AAGACAGAGG ATATGGAGAG 
AAGCTTCTGG GATATGATGA TGAGATGGAT GTCACGGAGG TGGTGGATAT CAGTCTGCCA 
CCCAAACAGC AGGAGGATCG GCAGAGGGGG GAGGCCCTTC GGCAGCTGCT CGTCAATCGT 
TACTATGGCT TCAGGAGTGG TGGGCGGAGA GAAAGTCTGA CCTCGTTTGG TAACTCCACC 
CTAACTCCTG TCGGGCCAAT CAAGAGCCAG CCAGGGGGGT TGAGCAGGGC TGAGATGTCA 
TTAATGGAGG TGCAGTGTCA TCTAGACCGT GAAGGGGCAT CGGATCTTGT CATTGATCTC 
ATCATGAACA CTACCAGCGA CCGCGTCTTC CATGAAAGCA TTCTGTTGGC CATCGCACTG 
TTGGAGGGCG GAAACACCAT CATACAGCAT TCCTTCTTCA AACGTTTAAC AGAAGACAAA 
AATTCAGAGA AGTTTTTCAG GGTGTTTTAT GACAGAATGA AAGTGGCTCA GGTGGAGATC 
AAAGCCACGG TTACTGTCAA CACTAGTGAC CTCGGCAACA AGCGGCGTGA CGACAACACT 
CCAGACAAAG ATACTCCACA ACGCCGCAGA GGTAAAGACT CCGGTGTGGT GGTGACAGAT 
GATGCTCGTG AGCAGCTGTT GGAGGCTTCA GCTGCGACTA AGAAGGCCTT TGGTTCATAT 
CGTCGAGATG CTGATCCCGA GGAGACGTTT GGCACGGCTG ATGGAGATAA AGGAGGTGGA 
GATAAAGGCA CAGAGCAGGG CGAGATGAGC CCTGTCATCC TCATAATGCA GCCAATCCTG 
CGCTTCCTGC AGCTGCTGTG CGAAAACCAC AATCGAGACC TGCAGAATTT TCTGCGCTGT 
CAGAACAATA AAAACAACTA TAATCTGGTG TGTGAGACGC TGCAGTTCCT GGACTGTATC 
TGTGGCAGCA CAACAGGAGG CCTGGGGCTG TTAGGACTCT ACATCAACCA GCATAACGTA 
GCGCTCATTA ATCAGACTGT AGAGAGCCTC ACTGAATACT GTCAAGGTCC CTGCCATGAC 
AATCAGAATT GCATTGCCAC CCATGAGTCT AATGGCATCG ACATCATTAT TGCACTGATT 
CTGAATGATA TTAATCCACT GGGCAGGAAG AGAATGGATC TGGTTCTAGA ACTAAAGAAT 
AATGCATCTA AGCTGCTGCT GGCCATCATG GAGAGCCGTC ATGACAGTGA GAATGCAGAA 
AGAATTCTGT ACAACATGAG ACCAAAAGAA CTAGTGGAGG TGATGAAAAT GGCCTACCAG 
CAGGGGGAGG CAGAGTTTGA GGATGAGGAA CAGGAGAATG GAGAGGATCA TGCAGCATCG 
CCTCGCAATG TTGGACACAA CATCTATATT CTTGCACATC AGCTGTCGCG TCACAATAAG 
GAATTGCAAA TACTACTGAA ACCAGGTGGA GAGGACCAGG CCCTGGAGTA TTACACAAAA 
CACACCTCAC AGATAGAGAT AGTTCGACAG GATCGCACTA TGGAACAGAT TGTGTTTCCT 
GTACCAAATA TCTGCTCTTT TCTGACAAAT GAGTCCAAGC TGCGTGTGTA CTATGGTACA 
GAGAGAGACG AGCAAGGCAG TAAAATAAAT GACTTCTTCT TGCATGCAGA CGACCTCTTT 
AATGAAATGA GGTGGCAGAA AAAACTAAGA GCTCAGCCAG TGCTGTACTG GTGCAGCAGG 
AACATGTCGT TTTGGAGTAA CGTTTCCTTT AATCTGGCGG TGCTCATTAA TTTGCTGGTG 
GCATTTTTCT ACCCTCTGGA TGGAGTGAGC GAAAGCCAGC TGGAACCTTC TCTGTCTCTG 
TTGCTGTGGG TTTGTTTGTT GGGCTCACTG GGCTTCGTGT TGATGTCCCC GAGGCCAAAT 
GCAGTGCGGG TGCTGGTCAT CTCCACTGTC CTGCAGTTGG GCTTCTCTGT AGGGCTGCAG 
CACATGCTCA CACTCCTGGG AGCTTTCAAT GTTTGTAATA AGATCGTGTT CATGCTGAGC 
TTTGTGGGTA ACCGTGGGTC ATTCACCCGG GGTTATCGGG TGATGGTCAT GGATAGAGAG 
TTCCTGTTTC ATCTGCTGTA CCTTCTCATC TGCATACTGG GCCTTTTTGG ACACGTCTTC 
TTTTACAGTC TTCTGTTGTT TGACCTGGTG AACCGGGAAG AGACTCTTCT GAACGTCATC 
AAGAGTGTGA CTCGAAACGG CCGCTCCATC GTCTTGACCG CAGTGCTGGG CCTCATCCTG 
GTCTACCTCT TTTCTATTGT TGGATATATG TTTTTCAAAG ATGACTTCAT CCTGGAGGTG 
AACCGCATCT CCAACGGCAC TCTGGAGGAG GGGGTGACTT TACCTGCTGG TTTTTGTGAT 
GCGAGAAACG GGTCGTGTCT TTCTGATGAT GTTGACGAGG ATGACGTGGA GCGCGCGTGT 
GATTCTCTAT GGATGTGTAT GATCACTGTA CTCAGTCATG GCCTCAGGAG CGGTGGAGGA 
GTTGGAGATG TCCTGCGCAA ACCGTCTAAA GAGGAGCGTC TGTTCGCCGC ACGGGTGGTG 
TACGATCTCC TGTTCTTCTT CCTGGTGATC ATCATTGTGT TGAATCTGAT CTTCGGTGTG 
ATCATTGACA CATTTGCAGA TCTGAGAAGC GAGAAGCAGA GAAAAGAGGA GATCTTGAAG 
ACCACATGCT TCATCTGTGG TCTGGAGAGA GATAAGTTTG ATAATAAAAC GGTGACGTTT 
GAAGAGCACA TCAAGGAGGA GCACAACCTG TGGCATTACC TCTTCTTCAT AGTGCTGGTG 
CGGGTCAAAG ACTCCACCGA ATACACGGGA CCCGAGAGCT ACGTCGCTCA GATGATCAAG 
GAGCACAATC TGGACTGGTT TCCTCGTATG AGGGCGATGT CTCTGGTCAG CAGTGATGGA 
GAAGGTGAAC AGAATGAACT GCGCAGCTTA CAGGAGAAAC TCGAGTCCAC AATGAGGCTC 
GTCTCTAATC TTACCAATCA GCTCACAGAG CTCAAAGAGC AGATGACTGA GCAAAGGAAA 
CACAAGCAGA GGCTCGGTCT GCTGGGAAAC CCCGCCCACC TCAACATCAA CCCACAGCAG 
CCAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itpr1a ( XM_021474613.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330288.1 Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X10, mRNA.

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CloneID	ODa146872
Clone ID Related Accession (Same CDS sequence)	XM_021474613.1
Accession Version	XM_021474613.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395382.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACAT TACAAGGTGC TGGAGTTTTT GCCATGGTGG ACAAATTGTC TAGTTTCCTG 
CACATTGGGG ACGTTTGTTC CCTTTATGCT GAAGGCTCTA CTAGTGGCTT CATCAGCACT 
CTGGGGTTGG TTGATGACCG ATGTGTTGTT CAACCAGATG CCGGAGACCT GAATAACCCA 
CCTAAAAAGT TTCGGGACTG TCTGTTCAGA CTGTGTCCCA TGAACAGATA TTCGGCACAG 
AAACAGTTCT GGAAAGCTGC CAAACCTGGA GGAACTAGCA CCACAGATAC TGTCCTGCTT 
AACAAACTCC ATCATGCTGC TGATTTAGAG AAGAAACAAA ATGATTCTGA GAACAAAAAG 
CTGCTTGGAA CCGTCATTCA GTATGGCAAT GTCATCCAGC TTCTCCATCT GAAGAGTAAT 
AAATATCTGA CGGTGAATAA GCGTTTGCCG GCTCTTCTTG AGAAGAATGC CATGCGTGTG 
ACTCTGGACG CTGCAGGGAA TGAAGGCTCG TGGTTTTACA TCCAGCCCTT CTATAAGCTG 
CGCTCTATAG GGGATAATGT GGTCATTGGA GATAAGGTCG TGTTAAACCC TGTCAATGCC 
GGACAGCCGC TGCACGCCAG CAGCCATCAG CTTGTGGATA ATCCAGGCTG CAATGAAGTG 
AATTCAGTGA ACTGCACCAC CAGTTGGAAA ATCGTTCTCT TCATGAAATG GAGCGACAAC 
CAGGAGATTG TCTTGAAAGG AGGTGACGTG GTGAGGCTGT TTCATGCAGA GCAGGAGAAG 
TTTCTGACAT GTGATGAGCA TCGGAAAAAG CAGTATGTGT TTCTGCGTAC CACCGGACGC 
CAGTCTGCAA CCTCCGCCAC TAGTAGCAAA GCACTCTGGG AGGTAGAGGT GGTTCAGCAT 
GATCCCTGCC GAGGCGGAGC CGGCTACTGG AACAGCCTCT TTAGGTTTAA ACACTTAGCC 
ACCGGCCATT ACCTTGCTGC AGAGGTAAAT CCAGACTATG AAGAAGAATG CCTGGAGTCC 
CGCTCCTCTA TGGATTCGGA GCATGAAGTG ATTCGGGCTC GTGCACGTAA CCCTCAGGAT 
AAGGTGATGT ACACACTGGT GTCTGTTCCT GATGGAAATG ACATATCCTC CATCTTTGAG 
CTTGACCCTA CAACTTTGAG GGGCGGAGAC TCACTTGTGC CAAGAAACTC GTATGTGAGG 
CTCAGGCATC TCTGCACCAA CACGTGGGTC CACAGCACCA ACCAGCCAAT AGATAAAGAG 
GAAGAGAAAC CGGTCATGCT CAGAATAGGA ACGTCTCCTC TGAAGGAAGA TAAAGAGGCG 
TTTGCTATAG TACCAGTCTC TCCAGCTGAG GTTCGAGATC TGGATTTTGC TAATGATGCT 
AGTAAAGTGT TGGCCTCCAT TGCTGCCAAA CTGGAGAAGG GCACCATCAC CCAGAATGAG 
AGGAGGTCAG TGACGAAGCT GTTGGAGGAT CTGGTGTATT TTGTGGTGGA CATACCCAGC 
AGTGCTCAAG ATGTGCTGGA GATCACAGTC AACAAACCCA ACAGAGAGCG ACAGAAACTC 
ATGAGAGAGC AGAACATTCT TAAACAGATC TTCAAGCTGC TGCAGGCGCC ATTTACAGAC 
AGCGGGGACG GCCCTATGTT GCGATTAGAA GAGCTCGCAG ACCAGCGTCA TGCACCCTTT 
CGACACATTT GCAGACTCTG TTACAGAGTC CTACGTTACT CACAACAGGA CTATCGCAAA 
AATCAGGAGT ACATAGCAAA GCAGTTCCGC TTCATGCAGA AGCAGATAGG ATATGATGTG 
CTGGCTGAAG ACACAATTAC AGCCCTTCTG CACAACAACA GGAAGCTCCT AGAGAAACAC 
ATCACAGCAG CTGAAATAGA TACCTTTGTC AGTCTGGTCC GCAAGAACAG AGAGCCCAGA 
TTTCTGGATT ACTTGTCAGA TCTGTGTGTG TCGATGAATA AATCCATCCC GGTCACTCAA 
GAGCTCATCT GTAATGCTGT GCTGGACCCA GCCAATGCAG ACATTCTCAT CGAAACCAAG 
CTTGTGTTGT CACGATTTGA GGTGGCAGGA ACGGTGTTGG GTGAAGGTGC GGAGGAGGAA 
GAGGAGGATG AAGAGGAGGT CTGGCTGTTC TGGAAGGACA GTGGAGGAGA GGTGAAGAGT 
AAAAGCATAC GAGAGCTTGC ACAGGACGCA AAAGATGGAC ATACAGAGGA CCAGGAAGTC 
ATCAATTATT ACAGATATCA GCTGAATTTG TTTGCTCGGA TGTGTCTGGA TCGACAGTAT 
CTTGCGATTA ACAAGATATC AGCACAGCTG GATGTGGATC TGATTCTGCG CTGCATGTCA 
GATGAAGATT TGCCCTTTGA CCTCAGAGCA TCGTTCTGCC GCATGATGCT GCACATGCAT 
GTAGACCGTG ACCCTCAGGA GCAGGTCACC CCGGTCAAAT ATGCTCGACT GTGGTCTGAA 
ATCCCATCAC AAATTTCCAT TGATGATTAT GATAATGATG GGACGTCACG TGATGAAGTG 
AAAGAGCGCT TCTCTCAGAC GATGGAGTTT GTGGAGAATT ACCTGAGGGA TGTTGTCTGC 
CAGAGTTTCC CGTTCTCTGA TAAGGAGAAA AACAAACTCA CTTTTGAGGT GGTGAACCTG 
GCAAGAAACC TGATCTATTT TGGTTTCTAT AACTTCAGTG ATCTGCTAAG GTTAACAAAA 
ATCCTATTGG CAATCCTGGA CTGTGTCCAC ATAAGCACTC CCTTCCACAT CAAGCTGGAT 
AGAGAACCTG GTAAAGGAAG TAACGTGATG CGCTCCATCC ATGGGGTGGG AGAACTGATG 
ACACAGGTAG TGTTGAGGGG TGGGGGATTT CTGCCAGCCT CCAGCCACAA TCCTCCTGAC 
AGAGACGAAG TGAAAGCACA AAGTGAACCT CAGAAACAAG ACATACTGGT GATGGACACT 
AAACTCAAGA TCATCGAGAT CCTGCAGTTT ATTCTGAATG TGCGGTTGGA CTACAGGATC 
TCCTGCCTCC TCTGTATCTT TAAGACAGAG TTTGATGAAA GTAACTCTCA AAGTGAACCA 
TCTTTAAGCC CAGAATCTCC TGCCAGCGTT CAAGGTGCGC TAGACTTTGA GCACATTGAA 
GAGCAGGCGG AGGGTATTTT TGGAGGAAGT GAAGAGAACA CCCCGCTGGA TCTTGATGAT 
CATGGTGGAC GGACATTTCT GAGGGTCCTG CTTCACCTGA CCATGCATGA CTATCCTCCG 
CTGGTATCTC GAGCTCTTCA CCTGCTCTTC AGACACTTTA GCCAACGCCA GGAGGTCCTA 
CAGGCCTTCA AACAGGTTCA GCTCCTGGTG ACCAGTCAGG ATGTGGAAAA CTATAAACAG 
ATCAAGTCAG ATTTGGACCA GCTGAGGTCA ATAGTAGAGA AATCAGAGTT GTGGGTGTAT 
AAGAGACAAG GCTCAGACTC AGGACTGGAT GCTGGAGAAG TCACCCCTGA AGCCCACCAC 
AAGGGTGTCA TCAACTCGAG TAGATCAAAC AAACCCAAAG TGGAAAGTAC CAGCAGCTCG 
AACTACAGAG TCGTGAAAGA GATCCTGCTG AGGCTGAGCA AACTGTGTGT TTTGGAGGGC 
ATCTCTGGCA AGAAGAATAA GAAACAGCAG CAGCGGCTCC TCAGGAACAT GGGAGCTCAC 
AGTGTGGTCC TCGAACTGTT GCAAATCCCT TATGAGAAGG GGGAGGATGT CCAGATGCAG 
GAGATCATGA CCTTAGCACA TCAGTTCCTT CAAAACTTCT GTGCTGGAAA TCAGCAGAAC 
CAGGCACTTC TACACAAACA CATCAACCTG TTCCTCAACC CTGGGATCCT GGAGGCGGTG 
ACCATGCAGC ATATCTTCAT GAATAATTTC CAGTTGTGTA GTGAGATTAA TGAGCGGGTC 
GTGCAGCATT TTGTGCACTG CATCGAGACA CACGGCCGCA GCGTGCACTA CCTCAAGTTC 
CTGCAGACCA TTGTCAAGGC AGAGAACAAG TTCATCAAGA AATGCCAAGA CATCGTCATG 
GCTGAGCTGG TGACTGCAGG TGAGGATGTG CTGGTGTTCT ATAATGACCG GGCGTCTTTT 
CAGTCATTGG TTCAGATGAT GCGTCTTGAG CGGGAACGAC TTGATGAGAG CAGTGCACTC 
AGGTATCACA TTCACCTGGT GGAGTTGCTG GCTGTGTGCA CTGAAGGCAA GAACGTCTAC 
ACTGAGATCA AATGCAACTC GCTGCTCCCT CTCGATGATA TTGTACGTGT GGTCACACAT 
GAGGACTGCA TCCCTGAGGT GAAGATGGCC TACGTGAACT TCCTGAATCA CTGTTATGTT 
GATACAGAGG TTGAGATGAA GGAGATCTAC ACCAGCAATC ACATGTGGAA ACTTTTTGAT 
GATTTTCTGG TGGACGTCTG CAGAGTGTGC AATAACACAA GTGACCGTAA ACACGCTGAC 
ACAGTTCTGG AGAGATACGT TACAGAGACC ATAATGAGCA TCGTCACCAC CTTTTTCAGC 
TCACCATTCT CAGACCAGAG CACAAGTTTG CAGACCCGGC AGCCGGTGTT TGTGCAGCTG 
CTGCAGGGAG TGTTTCGTGT ATATCACTGC AACTGGCTTC TCCCAGGTCA GAAGGGGAAT 
GTGGAAGCCT GCATCAAAGT GCTGAGTGAC GTGGCCAAAG GCAGAGCCAT AGCCATTCCT 
GTGGATCTGG ACTGTCAGGT GAATAATCTG TTCATGAAGT CCAACAACAT TGTGCAGAAA 
ACAGCCCTCA GCTGGAGACT CTCTGTCCGT AACGCGACTC GGAGAGACTC TGTTCTGACC 
ACCTCTCGAG ACTATCGCAA CATCATTGAA AGACTACAGG ATATAGTGTC TGCATTGGAG 
GAGCGGTTGA GGCCTCTGGT TCAGGCTGAG TTGTCAGTGT TGGTGGATGT TCTCCATCGC 
CCAGAGCTGC TGTTTCCAGA GCACACTGAA GCCCACCGCA AGTGTGAGAG TGGAGGCTTC 
ATCTGCAAGT TGATAAAACA CACTAAGCAG CTTCTAGAAG AAAACGAAGA GAGGCTTTGC 
ATCAAAGTCC TTCAGACACT CCGAGAGATG ATGACCAAAG ACAGAGGATA TGGAGAGAAG 
CTTCTGGGAT ATGATGATGA GATGGATGTC ACGGAGGTGG TGGATATCAG TCTGCCACCC 
AAACAGCAGG AGGATCGGCA GAGGGGGGAG GCCCTTCGGC AGCTGCTCGT CAATCGTTAC 
TATGGCTTCA GGAGTGGTGG GCGGAGAGAA AGTCTGACCT CGTTTGGTAA CTCCACCCTA 
ACTCCTGTCG GGCCAATCAA GAGCCAGCCA GGGGGGTTGA GCAGGGCTGA GATGTCATTA 
ATGGAGGTGC AGTGTCATCT AGACCGTGAA GGGGCATCGG ATCTTGTCAT TGATCTCATC 
ATGAACACTA CCAGCGACCG CGTCTTCCAT GAAAGCATTC TGTTGGCCAT CGCACTGTTG 
GAGGGCGGAA ACACCATCAT ACAGCATTCC TTCTTCAAAC GTTTAACAGA AGACAAAAAT 
TCAGAGAAGT TTTTCAGGGT GTTTTATGAC AGAATGAAAG TGGCTCAGGT GGAGATCAAA 
GCCACGGTTA CTGTCAACAC TAGTGACCTC GGCAACAAGC GGCGTGACGA CAACACTCCA 
GACAAAGATA CTCCACAACG CCGCAGAGGT AAAGACTCCG GTGTGGTGGT GACAGATGAT 
GCTCGTGAGC AGCTGTTGGA GGCTTCAGCT GCGACTAAGA AGGCCTTTGG TTCATATCGT 
CGAGATGCTG ATCCCGAGGA GACGTTTGGC ACGGCTGATG GAGATAAAGG AGGTGGAGAT 
AAAGGCACAG AGCAGGGCGA GATGAGCCCT GTCATCCTCA TAATGCAGCC AATCCTGCGC 
TTCCTGCAGC TGCTGTGCGA AAACCACAAT CGAGACCTGC AGAATTTTCT GCGCTGTCAG 
AACAATAAAA ACAACTATAA TCTGGTGTGT GAGACGCTGC AGTTCCTGGA CTGTATCTGT 
GGCAGCACAA CAGGAGGCCT GGGGCTGTTA GGACTCTACA TCAACCAGCA TAACGTAGCG 
CTCATTAATC AGACTGTAGA GAGCCTCACT GAATACTGTC AAGGTCCCTG CCATGACAAT 
CAGAATTGCA TTGCCACCCA TGAGTCTAAT GGCATCGACA TCATTATTGC ACTGATTCTG 
AATGATATTA ATCCACTGGG CAGGAAGAGA ATGGATCTGG TTCTAGAACT AAAGAATAAT 
GCATCTAAGC TGCTGCTGGC CATCATGGAG AGCCGTCATG ACAGTGAGAA TGCAGAAAGA 
ATTCTGTACA ACATGAGACC AAAAGAACTA GTGGAGGTGA TGAAAATGGC CTACCAGCAG 
GGGGAGGCAG AGTTTGAGGA TGAGGAACAG GAGAATGGAG AGGATCATGC AGCATCGCCT 
CGCAATGTTG GACACAACAT CTATATTCTT GCACATCAGC TGTCGCGTCA CAATAAGGAA 
TTGCAAATAC TACTGAAACC AGGTGGAGAG GACCAGGCCC TGGAGTATTA CACAAAACAC 
ACCTCACAGA TAGAGATAGT TCGACAGGAT CGCACTATGG AACAGATTGT GTTTCCTGTA 
CCAAATATCT GCTCTTTTCT GACAAATGAG TCCAAGCTGC GTGTGTACTA TGGTACAGAG 
AGAGACGAGC AAGGCAGTAA AATAAATGAC TTCTTCTTGC ATGCAGACGA CCTCTTTAAT 
GAAATGAGGT GGCAGAAAAA ACTAAGAGCT CAGCCAGTGC TGTACTGGTG CAGCAGGAAC 
ATGTCGTTTT GGAGTAACGT TTCCTTTAAT CTGGCGGTGC TCATTAATTT GCTGGTGGCA 
TTTTTCTACC CTCTGGATGG AGTGAGCGAA AGCCAGCTGG AACCTTCTCT GTCTCTGTTG 
CTGTGGGTTT GTTTGTTGGG CTCACTGGGC TTCGTGTTGA TGTCCCCGAG GCCAAATGCA 
GTGCGGGTGC TGGTCATCTC CACTGTCCTG CAGTTGGGCT TCTCTGTAGG GCTGCAGCAC 
ATGCTCACAC TCCTGGGAGC TTTCAATGTT TGTAATAAGA TCGTGTTCAT GCTGAGCTTT 
GTGGGTAACC GTGGGTCATT CACCCGGGGT TATCGGGTGA TGGTCATGGA TAGAGAGTTC 
CTGTTTCATC TGCTGTACCT TCTCATCTGC ATACTGGGCC TTTTTGGACA CGTCTTCTTT 
TACAGTCTTC TGTTGTTTGA CCTGGTGAAC CGGGAAGAGA CTCTTCTGAA CGTCATCAAG 
AGTGTGACTC GAAACGGCCG CTCCATCGTC TTGACCGCAG TGCTGGGCCT CATCCTGGTC 
TACCTCTTTT CTATTGTTGG ATATATGTTT TTCAAAGATG ACTTCATCCT GGAGGTGAAC 
CGCATCTCCA ACGGCACTCT GGAGGAGGGG GTGACTTTAC CTGCTGGTTT TTGTGATGCG 
AGAAACGGGT CGTGTCTTTC TGATGATGTT GACGAGGATG ACGTGGAGCG CGCGTGTGAT 
TCTCTATGGA TGTGTATGAT CACTGTACTC AGTCATGGCC TCAGGAGCGG TGGAGGAGTT 
GGAGATGTCC TGCGCAAACC GTCTAAAGAG GAGCGTCTGT TCGCCGCACG GGTGGTGTAC 
GATCTCCTGT TCTTCTTCCT GGTGATCATC ATTGTGTTGA ATCTGATCTT CGGTGTGATC 
ATTGACACAT TTGCAGATCT GAGAAGCGAG AAGCAGAGAA AAGAGGAGAT CTTGAAGACC 
ACATGCTTCA TCTGTGGTCT GGAGAGAGAT AAGTTTGATA ATAAAACGGT GACGTTTGAA 
GAGCACATCA AGGAGGAGCA CAACCTGTGG CATTACCTCT TCTTCATAGT GCTGGTGCGG 
GTCAAAGACT CCACCGAATA CACGGGACCC GAGAGCTACG TCGCTCAGAT GATCAAGGAG 
CACAATCTGG ACTGGTTTCC TCGTATGAGG GCGATGTCTC TGGTCAGCAG TGATGGAGAA 
GGTGAACAGA ATGAACTGCG CAGCTTACAG GAGAAACTCG AGTCCACAAT GAGGCTCGTC 
TCTAATCTTA CCAATCAGCT CACAGAGCTC AAAGAGCAGA TGACTGAGCA AAGGAAACAC 
AAGCAGAGGC TCGGTCTGCT GGGAAACCCC GCCCACCTCA ACATCAACCC ACAGCAGCCA 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330288.1
CDS	265..8430
Translation	MMTLQGAGVFAMVDKLSSFLHIGDVCSLYAEGSTSGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGTSTTDTVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDAAGNEGSWFYIQPFYKLRSIGDNVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCTTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDEHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEVNPDYEEECLESRSSMDSEHEVIRARARNPQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSPLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAAKLEKGTITQNERRSVTKLLEDLVYFVVDIPSSAQDVLEITVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRYSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDPANADILIETKLVLSRFEVAGTVLGEGAEEEEEDEEEVWLFWKDSGGEVKSKSIRELAQDAKDGHTEDQEVINYYRYQLNLFARMCLDRQYLAINKISAQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSQISIDDYDNDGTSRDEVKERFSQTMEFVENYLRDVVCQSFPFSDKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHISTPFHIKLDREPGKGSNVMRSIHGVGELMTQVVLRGGGFLPASSHNPPDRDEVKAQSEPQKQDILVMDTKLKIIEILQFILNVRLDYRISCLLCIFKTEFDESNSQSEPSLSPESPASVQGALDFEHIEEQAEGIFGGSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALHLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSDSGLDAGEVTPEAHHKGVINSSRSNKPKVESTSSSNYRVVKEILLRLSKLCVLEGISGKKNKKQQQRLLRNMGAHSVVLELLQIPYEKGEDVQMQEIMTLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAVTMQHIFMNNFQLCSEINERVVQHFVHCIETHGRSVHYLKFLQTIVKAENKFIKKCQDIVMAELVTAGEDVLVFYNDRASFQSLVQMMRLERERLDESSALRYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKMAYVNFLNHCYVDTEVEMKEIYTSNHMWKLFDDFLVDVCRVCNNTSDRKHADTVLERYVTETIMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQGVFRVYHCNWLLPGQKGNVEACIKVLSDVAKGRAIAIPVDLDCQVNNLFMKSNNIVQKTALSWRLSVRNATRRDSVLTTSRDYRNIIERLQDIVSALEERLRPLVQAELSVLVDVLHRPELLFPEHTEAHRKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKLLGYDDEMDVTEVVDISLPPKQQEDRQRGEALRQLLVNRYYGFRSGGRRESLTSFGNSTLTPVGPIKSQPGGLSRAEMSLMEVQCHLDREGASDLVIDLIMNTTSDRVFHESILLAIALLEGGNTIIQHSFFKRLTEDKNSEKFFRVFYDRMKVAQVEIKATVTVNTSDLGNKRRDDNTPDKDTPQRRRGKDSGVVVTDDAREQLLEASAATKKAFGSYRRDADPEETFGTADGDKGGGDKGTEQGEMSPVILIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINQHNVALINQTVESLTEYCQGPCHDNQNCIATHESNGIDIIIALILNDINPLGRKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVMKMAYQQGEAEFEDEEQENGEDHAASPRNVGHNIYILAHQLSRHNKELQILLKPGGEDQALEYYTKHTSQIEIVRQDRTMEQIVFPVPNICSFLTNESKLRVYYGTERDEQGSKINDFFLHADDLFNEMRWQKKLRAQPVLYWCSRNMSFWSNVSFNLAVLINLLVAFFYPLDGVSESQLEPSLSLLLWVCLLGSLGFVLMSPRPNAVRVLVISTVLQLGFSVGLQHMLTLLGAFNVCNKIVFMLSFVGNRGSFTRGYRVMVMDREFLFHLLYLLICILGLFGHVFFYSLLLFDLVNREETLLNVIKSVTRNGRSIVLTAVLGLILVYLFSIVGYMFFKDDFILEVNRISNGTLEEGVTLPAGFCDARNGSCLSDDVDEDDVERACDSLWMCMITVLSHGLRSGGGVGDVLRKPSKEERLFAARVVYDLLFFFLVIIIVLNLIFGVIIDTFADLRSEKQRKEEILKTTCFICGLERDKFDNKTVTFEEHIKEEHNLWHYLFFIVLVRVKDSTEYTGPESYVAQMIKEHNLDWFPRMRAMSLVSSDGEGEQNELRSLQEKLESTMRLVSNLTNQLTELKEQMTEQRKHKQRLGLLGNPAHLNINPQQPA

Target ORF information:

RefSeq Version	XM_021474613.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474613.1

ORF Insert Sequence:

ATGATGACAT TACAAGGTGC TGGAGTTTTT GCCATGGTGG ACAAATTGTC TAGTTTCCTG 
CACATTGGGG ACGTTTGTTC CCTTTATGCT GAAGGCTCTA CTAGTGGCTT CATCAGCACT 
CTGGGGTTGG TTGATGACCG ATGTGTTGTT CAACCAGATG CCGGAGACCT GAATAACCCA 
CCTAAAAAGT TTCGGGACTG TCTGTTCAGA CTGTGTCCCA TGAACAGATA TTCGGCACAG 
AAACAGTTCT GGAAAGCTGC CAAACCTGGA GGAACTAGCA CCACAGATAC TGTCCTGCTT 
AACAAACTCC ATCATGCTGC TGATTTAGAG AAGAAACAAA ATGATTCTGA GAACAAAAAG 
CTGCTTGGAA CCGTCATTCA GTATGGCAAT GTCATCCAGC TTCTCCATCT GAAGAGTAAT 
AAATATCTGA CGGTGAATAA GCGTTTGCCG GCTCTTCTTG AGAAGAATGC CATGCGTGTG 
ACTCTGGACG CTGCAGGGAA TGAAGGCTCG TGGTTTTACA TCCAGCCCTT CTATAAGCTG 
CGCTCTATAG GGGATAATGT GGTCATTGGA GATAAGGTCG TGTTAAACCC TGTCAATGCC 
GGACAGCCGC TGCACGCCAG CAGCCATCAG CTTGTGGATA ATCCAGGCTG CAATGAAGTG 
AATTCAGTGA ACTGCACCAC CAGTTGGAAA ATCGTTCTCT TCATGAAATG GAGCGACAAC 
CAGGAGATTG TCTTGAAAGG AGGTGACGTG GTGAGGCTGT TTCATGCAGA GCAGGAGAAG 
TTTCTGACAT GTGATGAGCA TCGGAAAAAG CAGTATGTGT TTCTGCGTAC CACCGGACGC 
CAGTCTGCAA CCTCCGCCAC TAGTAGCAAA GCACTCTGGG AGGTAGAGGT GGTTCAGCAT 
GATCCCTGCC GAGGCGGAGC CGGCTACTGG AACAGCCTCT TTAGGTTTAA ACACTTAGCC 
ACCGGCCATT ACCTTGCTGC AGAGGTAAAT CCAGACTATG AAGAAGAATG CCTGGAGTCC 
CGCTCCTCTA TGGATTCGGA GCATGAAGTG ATTCGGGCTC GTGCACGTAA CCCTCAGGAT 
AAGGTGATGT ACACACTGGT GTCTGTTCCT GATGGAAATG ACATATCCTC CATCTTTGAG 
CTTGACCCTA CAACTTTGAG GGGCGGAGAC TCACTTGTGC CAAGAAACTC GTATGTGAGG 
CTCAGGCATC TCTGCACCAA CACGTGGGTC CACAGCACCA ACCAGCCAAT AGATAAAGAG 
GAAGAGAAAC CGGTCATGCT CAGAATAGGA ACGTCTCCTC TGAAGGAAGA TAAAGAGGCG 
TTTGCTATAG TACCAGTCTC TCCAGCTGAG GTTCGAGATC TGGATTTTGC TAATGATGCT 
AGTAAAGTGT TGGCCTCCAT TGCTGCCAAA CTGGAGAAGG GCACCATCAC CCAGAATGAG 
AGGAGGTCAG TGACGAAGCT GTTGGAGGAT CTGGTGTATT TTGTGGTGGA CATACCCAGC 
AGTGCTCAAG ATGTGCTGGA GATCACAGTC AACAAACCCA ACAGAGAGCG ACAGAAACTC 
ATGAGAGAGC AGAACATTCT TAAACAGATC TTCAAGCTGC TGCAGGCGCC ATTTACAGAC 
AGCGGGGACG GCCCTATGTT GCGATTAGAA GAGCTCGCAG ACCAGCGTCA TGCACCCTTT 
CGACACATTT GCAGACTCTG TTACAGAGTC CTACGTTACT CACAACAGGA CTATCGCAAA 
AATCAGGAGT ACATAGCAAA GCAGTTCCGC TTCATGCAGA AGCAGATAGG ATATGATGTG 
CTGGCTGAAG ACACAATTAC AGCCCTTCTG CACAACAACA GGAAGCTCCT AGAGAAACAC 
ATCACAGCAG CTGAAATAGA TACCTTTGTC AGTCTGGTCC GCAAGAACAG AGAGCCCAGA 
TTTCTGGATT ACTTGTCAGA TCTGTGTGTG TCGATGAATA AATCCATCCC GGTCACTCAA 
GAGCTCATCT GTAATGCTGT GCTGGACCCA GCCAATGCAG ACATTCTCAT CGAAACCAAG 
CTTGTGTTGT CACGATTTGA GGTGGCAGGA ACGGTGTTGG GTGAAGGTGC GGAGGAGGAA 
GAGGAGGATG AAGAGGAGGT CTGGCTGTTC TGGAAGGACA GTGGAGGAGA GGTGAAGAGT 
AAAAGCATAC GAGAGCTTGC ACAGGACGCA AAAGATGGAC ATACAGAGGA CCAGGAAGTC 
ATCAATTATT ACAGATATCA GCTGAATTTG TTTGCTCGGA TGTGTCTGGA TCGACAGTAT 
CTTGCGATTA ACAAGATATC AGCACAGCTG GATGTGGATC TGATTCTGCG CTGCATGTCA 
GATGAAGATT TGCCCTTTGA CCTCAGAGCA TCGTTCTGCC GCATGATGCT GCACATGCAT 
GTAGACCGTG ACCCTCAGGA GCAGGTCACC CCGGTCAAAT ATGCTCGACT GTGGTCTGAA 
ATCCCATCAC AAATTTCCAT TGATGATTAT GATAATGATG GGACGTCACG TGATGAAGTG 
AAAGAGCGCT TCTCTCAGAC GATGGAGTTT GTGGAGAATT ACCTGAGGGA TGTTGTCTGC 
CAGAGTTTCC CGTTCTCTGA TAAGGAGAAA AACAAACTCA CTTTTGAGGT GGTGAACCTG 
GCAAGAAACC TGATCTATTT TGGTTTCTAT AACTTCAGTG ATCTGCTAAG GTTAACAAAA 
ATCCTATTGG CAATCCTGGA CTGTGTCCAC ATAAGCACTC CCTTCCACAT CAAGCTGGAT 
AGAGAACCTG GTAAAGGAAG TAACGTGATG CGCTCCATCC ATGGGGTGGG AGAACTGATG 
ACACAGGTAG TGTTGAGGGG TGGGGGATTT CTGCCAGCCT CCAGCCACAA TCCTCCTGAC 
AGAGACGAAG TGAAAGCACA AAGTGAACCT CAGAAACAAG ACATACTGGT GATGGACACT 
AAACTCAAGA TCATCGAGAT CCTGCAGTTT ATTCTGAATG TGCGGTTGGA CTACAGGATC 
TCCTGCCTCC TCTGTATCTT TAAGACAGAG TTTGATGAAA GTAACTCTCA AAGTGAACCA 
TCTTTAAGCC CAGAATCTCC TGCCAGCGTT CAAGGTGCGC TAGACTTTGA GCACATTGAA 
GAGCAGGCGG AGGGTATTTT TGGAGGAAGT GAAGAGAACA CCCCGCTGGA TCTTGATGAT 
CATGGTGGAC GGACATTTCT GAGGGTCCTG CTTCACCTGA CCATGCATGA CTATCCTCCG 
CTGGTATCTC GAGCTCTTCA CCTGCTCTTC AGACACTTTA GCCAACGCCA GGAGGTCCTA 
CAGGCCTTCA AACAGGTTCA GCTCCTGGTG ACCAGTCAGG ATGTGGAAAA CTATAAACAG 
ATCAAGTCAG ATTTGGACCA GCTGAGGTCA ATAGTAGAGA AATCAGAGTT GTGGGTGTAT 
AAGAGACAAG GCTCAGACTC AGGACTGGAT GCTGGAGAAG TCACCCCTGA AGCCCACCAC 
AAGGGTGTCA TCAACTCGAG TAGATCAAAC AAACCCAAAG TGGAAAGTAC CAGCAGCTCG 
AACTACAGAG TCGTGAAAGA GATCCTGCTG AGGCTGAGCA AACTGTGTGT TTTGGAGGGC 
ATCTCTGGCA AGAAGAATAA GAAACAGCAG CAGCGGCTCC TCAGGAACAT GGGAGCTCAC 
AGTGTGGTCC TCGAACTGTT GCAAATCCCT TATGAGAAGG GGGAGGATGT CCAGATGCAG 
GAGATCATGA CCTTAGCACA TCAGTTCCTT CAAAACTTCT GTGCTGGAAA TCAGCAGAAC 
CAGGCACTTC TACACAAACA CATCAACCTG TTCCTCAACC CTGGGATCCT GGAGGCGGTG 
ACCATGCAGC ATATCTTCAT GAATAATTTC CAGTTGTGTA GTGAGATTAA TGAGCGGGTC 
GTGCAGCATT TTGTGCACTG CATCGAGACA CACGGCCGCA GCGTGCACTA CCTCAAGTTC 
CTGCAGACCA TTGTCAAGGC AGAGAACAAG TTCATCAAGA AATGCCAAGA CATCGTCATG 
GCTGAGCTGG TGACTGCAGG TGAGGATGTG CTGGTGTTCT ATAATGACCG GGCGTCTTTT 
CAGTCATTGG TTCAGATGAT GCGTCTTGAG CGGGAACGAC TTGATGAGAG CAGTGCACTC 
AGGTATCACA TTCACCTGGT GGAGTTGCTG GCTGTGTGCA CTGAAGGCAA GAACGTCTAC 
ACTGAGATCA AATGCAACTC GCTGCTCCCT CTCGATGATA TTGTACGTGT GGTCACACAT 
GAGGACTGCA TCCCTGAGGT GAAGATGGCC TACGTGAACT TCCTGAATCA CTGTTATGTT 
GATACAGAGG TTGAGATGAA GGAGATCTAC ACCAGCAATC ACATGTGGAA ACTTTTTGAT 
GATTTTCTGG TGGACGTCTG CAGAGTGTGC AATAACACAA GTGACCGTAA ACACGCTGAC 
ACAGTTCTGG AGAGATACGT TACAGAGACC ATAATGAGCA TCGTCACCAC CTTTTTCAGC 
TCACCATTCT CAGACCAGAG CACAAGTTTG CAGACCCGGC AGCCGGTGTT TGTGCAGCTG 
CTGCAGGGAG TGTTTCGTGT ATATCACTGC AACTGGCTTC TCCCAGGTCA GAAGGGGAAT 
GTGGAAGCCT GCATCAAAGT GCTGAGTGAC GTGGCCAAAG GCAGAGCCAT AGCCATTCCT 
GTGGATCTGG ACTGTCAGGT GAATAATCTG TTCATGAAGT CCAACAACAT TGTGCAGAAA 
ACAGCCCTCA GCTGGAGACT CTCTGTCCGT AACGCGACTC GGAGAGACTC TGTTCTGACC 
ACCTCTCGAG ACTATCGCAA CATCATTGAA AGACTACAGG ATATAGTGTC TGCATTGGAG 
GAGCGGTTGA GGCCTCTGGT TCAGGCTGAG TTGTCAGTGT TGGTGGATGT TCTCCATCGC 
CCAGAGCTGC TGTTTCCAGA GCACACTGAA GCCCACCGCA AGTGTGAGAG TGGAGGCTTC 
ATCTGCAAGT TGATAAAACA CACTAAGCAG CTTCTAGAAG AAAACGAAGA GAGGCTTTGC 
ATCAAAGTCC TTCAGACACT CCGAGAGATG ATGACCAAAG ACAGAGGATA TGGAGAGAAG 
CTTCTGGGAT ATGATGATGA GATGGATGTC ACGGAGGTGG TGGATATCAG TCTGCCACCC 
AAACAGCAGG AGGATCGGCA GAGGGGGGAG GCCCTTCGGC AGCTGCTCGT CAATCGTTAC 
TATGGCTTCA GGAGTGGTGG GCGGAGAGAA AGTCTGACCT CGTTTGGTAA CTCCACCCTA 
ACTCCTGTCG GGCCAATCAA GAGCCAGCCA GGGGGGTTGA GCAGGGCTGA GATGTCATTA 
ATGGAGGTGC AGTGTCATCT AGACCGTGAA GGGGCATCGG ATCTTGTCAT TGATCTCATC 
ATGAACACTA CCAGCGACCG CGTCTTCCAT GAAAGCATTC TGTTGGCCAT CGCACTGTTG 
GAGGGCGGAA ACACCATCAT ACAGCATTCC TTCTTCAAAC GTTTAACAGA AGACAAAAAT 
TCAGAGAAGT TTTTCAGGGT GTTTTATGAC AGAATGAAAG TGGCTCAGGT GGAGATCAAA 
GCCACGGTTA CTGTCAACAC TAGTGACCTC GGCAACAAGC GGCGTGACGA CAACACTCCA 
GACAAAGATA CTCCACAACG CCGCAGAGGT AAAGACTCCG GTGTGGTGGT GACAGATGAT 
GCTCGTGAGC AGCTGTTGGA GGCTTCAGCT GCGACTAAGA AGGCCTTTGG TTCATATCGT 
CGAGATGCTG ATCCCGAGGA GACGTTTGGC ACGGCTGATG GAGATAAAGG AGGTGGAGAT 
AAAGGCACAG AGCAGGGCGA GATGAGCCCT GTCATCCTCA TAATGCAGCC AATCCTGCGC 
TTCCTGCAGC TGCTGTGCGA AAACCACAAT CGAGACCTGC AGAATTTTCT GCGCTGTCAG 
AACAATAAAA ACAACTATAA TCTGGTGTGT GAGACGCTGC AGTTCCTGGA CTGTATCTGT 
GGCAGCACAA CAGGAGGCCT GGGGCTGTTA GGACTCTACA TCAACCAGCA TAACGTAGCG 
CTCATTAATC AGACTGTAGA GAGCCTCACT GAATACTGTC AAGGTCCCTG CCATGACAAT 
CAGAATTGCA TTGCCACCCA TGAGTCTAAT GGCATCGACA TCATTATTGC ACTGATTCTG 
AATGATATTA ATCCACTGGG CAGGAAGAGA ATGGATCTGG TTCTAGAACT AAAGAATAAT 
GCATCTAAGC TGCTGCTGGC CATCATGGAG AGCCGTCATG ACAGTGAGAA TGCAGAAAGA 
ATTCTGTACA ACATGAGACC AAAAGAACTA GTGGAGGTGA TGAAAATGGC CTACCAGCAG 
GGGGAGGCAG AGTTTGAGGA TGAGGAACAG GAGAATGGAG AGGATCATGC AGCATCGCCT 
CGCAATGTTG GACACAACAT CTATATTCTT GCACATCAGC TGTCGCGTCA CAATAAGGAA 
TTGCAAATAC TACTGAAACC AGGTGGAGAG GACCAGGCCC TGGAGTATTA CACAAAACAC 
ACCTCACAGA TAGAGATAGT TCGACAGGAT CGCACTATGG AACAGATTGT GTTTCCTGTA 
CCAAATATCT GCTCTTTTCT GACAAATGAG TCCAAGCTGC GTGTGTACTA TGGTACAGAG 
AGAGACGAGC AAGGCAGTAA AATAAATGAC TTCTTCTTGC ATGCAGACGA CCTCTTTAAT 
GAAATGAGGT GGCAGAAAAA ACTAAGAGCT CAGCCAGTGC TGTACTGGTG CAGCAGGAAC 
ATGTCGTTTT GGAGTAACGT TTCCTTTAAT CTGGCGGTGC TCATTAATTT GCTGGTGGCA 
TTTTTCTACC CTCTGGATGG AGTGAGCGAA AGCCAGCTGG AACCTTCTCT GTCTCTGTTG 
CTGTGGGTTT GTTTGTTGGG CTCACTGGGC TTCGTGTTGA TGTCCCCGAG GCCAAATGCA 
GTGCGGGTGC TGGTCATCTC CACTGTCCTG CAGTTGGGCT TCTCTGTAGG GCTGCAGCAC 
ATGCTCACAC TCCTGGGAGC TTTCAATGTT TGTAATAAGA TCGTGTTCAT GCTGAGCTTT 
GTGGGTAACC GTGGGTCATT CACCCGGGGT TATCGGGTGA TGGTCATGGA TAGAGAGTTC 
CTGTTTCATC TGCTGTACCT TCTCATCTGC ATACTGGGCC TTTTTGGACA CGTCTTCTTT 
TACAGTCTTC TGTTGTTTGA CCTGGTGAAC CGGGAAGAGA CTCTTCTGAA CGTCATCAAG 
AGTGTGACTC GAAACGGCCG CTCCATCGTC TTGACCGCAG TGCTGGGCCT CATCCTGGTC 
TACCTCTTTT CTATTGTTGG ATATATGTTT TTCAAAGATG ACTTCATCCT GGAGGTGAAC 
CGCATCTCCA ACGGCACTCT GGAGGAGGGG GTGACTTTAC CTGCTGGTTT TTGTGATGCG 
AGAAACGGGT CGTGTCTTTC TGATGATGTT GACGAGGATG ACGTGGAGCG CGCGTGTGAT 
TCTCTATGGA TGTGTATGAT CACTGTACTC AGTCATGGCC TCAGGAGCGG TGGAGGAGTT 
GGAGATGTCC TGCGCAAACC GTCTAAAGAG GAGCGTCTGT TCGCCGCACG GGTGGTGTAC 
GATCTCCTGT TCTTCTTCCT GGTGATCATC ATTGTGTTGA ATCTGATCTT CGGTGTGATC 
ATTGACACAT TTGCAGATCT GAGAAGCGAG AAGCAGAGAA AAGAGGAGAT CTTGAAGACC 
ACATGCTTCA TCTGTGGTCT GGAGAGAGAT AAGTTTGATA ATAAAACGGT GACGTTTGAA 
GAGCACATCA AGGAGGAGCA CAACCTGTGG CATTACCTCT TCTTCATAGT GCTGGTGCGG 
GTCAAAGACT CCACCGAATA CACGGGACCC GAGAGCTACG TCGCTCAGAT GATCAAGGAG 
CACAATCTGG ACTGGTTTCC TCGTATGAGG GCGATGTCTC TGGTCAGCAG TGATGGAGAA 
GGTGAACAGA ATGAACTGCG CAGCTTACAG GAGAAACTCG AGTCCACAAT GAGGCTCGTC 
TCTAATCTTA CCAATCAGCT CACAGAGCTC AAAGAGCAGA TGACTGAGCA AAGGAAACAC 
AAGCAGAGGC TCGGTCTGCT GGGAAACCCC GCCCACCTCA ACATCAACCC ACAGCAGCCA 
GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itpr1a ( XM_021474614.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330289.1 Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X1, mRNA.

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CloneID	ODa146873
Clone ID Related Accession (Same CDS sequence)	XM_021474614.1
Accession Version	XM_021474614.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395382.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAATAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACT CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TAGAGGTGGT TCAGCATGAT CCCTGCCGAG GCGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GGTAAATCCA 
GACTATGAAG AAGAATGCCT GGAGTCCCGC TCCTCTATGG ATTCGGAGCA TGAAGTGATT 
CGGGCTCGTG CACGTAACCC TCAGGATAAG GTGATGTACA CACTGGTGTC TGTTCCTGAT 
GGAAATGACA TATCCTCCAT CTTTGAGCTT GACCCTACAA CTTTGAGGGG CGGAGACTCA 
CTTGTGCCAA GAAACTCGTA TGTGAGGCTC AGGCATCTCT GCACCAACAC GTGGGTCCAC 
AGCACCAACC AGCCAATAGA TAAAGAGGAA GAGAAACCGG TCATGCTCAG AATAGGAACG 
TCTCCTCTGA AGGAAGATAA AGAGGCGTTT GCTATAGTAC CAGTCTCTCC AGCTGAGGTT 
CGAGATCTGG ATTTTGCTAA TGATGCTAGT AAAGTGTTGG CCTCCATTGC TGCCAAACTG 
GAGAAGGGCA CCATCACCCA GAATGAGAGG AGGTCAGTGA CGAAGCTGTT GGAGGATCTG 
GTGTATTTTG TGGTGGACAT ACCCAGCAGT GCTCAAGATG TGCTGGAGAT CACAGTCAAC 
AAACCCAACA GAGAGCGACA GAAACTCATG AGAGAGCAGA ACATTCTTAA ACAGATCTTC 
AAGCTGCTGC AGGCGCCATT TACAGACAGC GGGGACGGCC CTATGTTGCG ATTAGAAGAG 
CTCGCAGACC AGCGTCATGC ACCCTTTCGA CACATTTGCA GACTCTGTTA CAGAGTCCTA 
CGTTACTCAC AACAGGACTA TCGCAAAAAT CAGGAGTACA TAGCAAAGCA GTTCCGCTTC 
ATGCAGAAGC AGATAGGATA TGATGTGCTG GCTGAAGACA CAATTACAGC CCTTCTGCAC 
AACAACAGGA AGCTCCTAGA GAAACACATC ACAGCAGCTG AAATAGATAC CTTTGTCAGT 
CTGGTCCGCA AGAACAGAGA GCCCAGATTT CTGGATTACT TGTCAGATCT GTGTGTGTCG 
ATGAATAAAT CCATCCCGGT CACTCAAGAG CTCATCTGTA ATGCTGTGCT GGACCCAGCC 
AATGCAGACA TTCTCATCGA AACCAAGCTT GTGTTGTCAC GATTTGAGGT GGCAGGAACG 
GTGTTGGGTG AAGGTGCGGA GGAGGAAGAG GAGGATGAAG AGGAGGTCTG GCTGTTCTGG 
AAGGACAGTG GAGGAGAGGT GAAGAGTAAA AGCATACGAG AGCTTGCACA GGACGCAAAA 
GATGGACATA CAGAGGACCA GGAAGTCATC AATTATTACA GATATCAGCT GAATTTGTTT 
GCTCGGATGT GTCTGGATCG ACAGTATCTT GCGATTAACA AGATATCAGC ACAGCTGGAT 
GTGGATCTGA TTCTGCGCTG CATGTCAGAT GAAGATTTGC CCTTTGACCT CAGAGCATCG 
TTCTGCCGCA TGATGCTGCA CATGCATGTA GACCGTGACC CTCAGGAGCA GGTCACCCCG 
GTCAAATATG CTCGACTGTG GTCTGAAATC CCATCACAAA TTTCCATTGA TGATTATGAT 
AATGATGGGA CGTCACGTGA TGAAGTGAAA GAGCGCTTCT CTCAGACGAT GGAGTTTGTG 
GAGAATTACC TGAGGGATGT TGTCTGCCAG AGTTTCCCGT TCTCTGATAA GGAGAAAAAC 
AAACTCACTT TTGAGGTGGT GAACCTGGCA AGAAACCTGA TCTATTTTGG TTTCTATAAC 
TTCAGTGATC TGCTAAGGTT AACAAAAATC CTATTGGCAA TCCTGGACTG TGTCCACATA 
AGCACTCCCT TCCACATCAA GCTGGATAGA GAACCTGGTA AAGGAAGTAA CGTGATGCGC 
TCCATCCATG GGGTGGGAGA ACTGATGACA CAGGTAGTGT TGAGGGGTGG GGGATTTCTG 
CCAGCCTCCA GCCACAATCC TCCTGACAGA GACGAAGTGA AAGCACAAAG TGAACCTCAG 
AAACAAGACA TACTGGTGAT GGACACTAAA CTCAAGATCA TCGAGATCCT GCAGTTTATT 
CTGAATGTGC GGTTGGACTA CAGGATCTCC TGCCTCCTCT GTATCTTTAA GACAGAGTTT 
GATGAAAGTA ACTCTCAAAG TGAACCATCT TTAAGCCCAG AATCTCCTGC CAGCGTTCAA 
GGTGCGCTAG ACTTTGAGCA CATTGAAGAG CAGGCGGAGG GTATTTTTGG AGGAAGTAGT 
GAAGAGAACA CCCCGCTGGA TCTTGATGAT CATGGTGGAC GGACATTTCT GAGGGTCCTG 
CTTCACCTGA CCATGCATGA CTATCCTCCG CTGGTATCTC GAGCTCTTCA CCTGCTCTTC 
AGACACTTTA GCCAACGCCA GGAGGTCCTA CAGGCCTTCA AACAGGTTCA GCTCCTGGTG 
ACCAGTCAGG ATGTGGAAAA CTATAAACAG ATCAAGTCAG ATTTGGACCA GCTGAGGTCA 
ATAGTAGAGA AATCAGAGTT GTGGGTGTAT AAGAGACAAG GCTCAGACTC AGGACTGGAT 
GCTGGAGAAG TCACCCCTGA AGCCCACCAC AAGGGTGTCA TCAACTCGAG TAGATCAAAC 
AAACCCAAAG TGGAAAGTAC CAGCAGCTCG AACTACAGAG TCGTGAAAGA GATCCTGCTG 
AGGCTGAGCA AACTGTGTGT TTTGGAGGGC ATCTCTGGCA AGAAGAATAA GAAACAGCAG 
CAGCGGCTCC TCAGGAACAT GGGAGCTCAC AGTGTGGTCC TCGAACTGTT GCAAATCCCT 
TATGAGAAGG GGGAGGATGT CCAGATGCAG GAGATCATGA CCTTAGCACA TCAGTTCCTT 
CAAAACTTCT GTGCTGGAAA TCAGCAGAAC CAGGCACTTC TACACAAACA CATCAACCTG 
TTCCTCAACC CTGGGATCCT GGAGGCGGTG ACCATGCAGC ATATCTTCAT GAATAATTTC 
CAGTTGTGTA GTGAGATTAA TGAGCGGGTC GTGCAGCATT TTGTGCACTG CATCGAGACA 
CACGGCCGCA GCGTGCACTA CCTCAAGTTC CTGCAGACCA TTGTCAAGGC AGAGAACAAG 
TTCATCAAGA AATGCCAAGA CATCGTCATG GCTGAGCTGG TGACTGCAGG TGAGGATGTG 
CTGGTGTTCT ATAATGACCG GGCGTCTTTT CAGTCATTGG TTCAGATGAT GCGTCTTGAG 
CGGGAACGAC TTGATGAGAG CAGTGCACTC AGGTATCACA TTCACCTGGT GGAGTTGCTG 
GCTGTGTGCA CTGAAGGCAA GAACGTCTAC ACTGAGATCA AATGCAACTC GCTGCTCCCT 
CTCGATGATA TTGTACGTGT GGTCACACAT GAGGACTGCA TCCCTGAGGT GAAGATGGCC 
TACGTGAACT TCCTGAATCA CTGTTATGTT GATACAGAGG TTGAGATGAA GGAGATCTAC 
ACCAGCAATC ACATGTGGAA ACTTTTTGAT GATTTTCTGG TGGACGTCTG CAGAGTGTGC 
AATAACACAA GTGACCGTAA ACACGCTGAC ACAGTTCTGG AGAGATACGT TACAGAGACC 
ATAATGAGCA TCGTCACCAC CTTTTTCAGC TCACCATTCT CAGACCAGAG CACAAGTTTG 
CAGACCCGGC AGCCGGTGTT TGTGCAGCTG CTGCAGGGAG TGTTTCGTGT ATATCACTGC 
AACTGGCTTC TCCCAGGTCA GAAGGGGAAT GTGGAAGCCT GCATCAAAGT GCTGAGTGAC 
GTGGCCAAAG GCAGAGCCAT AGCCATTCCT GTGGATCTGG ACTGTCAGGT GAATAATCTG 
TTCATGAAGT CCAACAACAT TGTGCAGAAA ACAGCCCTCA GCTGGAGACT CTCTGTCCGT 
AACGCGACTC GGAGAGACTC TGTTCTGACC ACCTCTCGAG ACTATCGCAA CATCATTGAA 
AGACTACAGG ATATAGTGTC TGCATTGGAG GAGCGGTTGA GGCCTCTGGT TCAGGCTGAG 
TTGTCAGTGT TGGTGGATGT TCTCCATCGC CCAGAGCTGC TGTTTCCAGA GCACACTGAA 
GCCCACCGCA AGTGTGAGAG TGGAGGCTTC ATCTGCAAGT TGATAAAACA CACTAAGCAG 
CTTCTAGAAG AAAACGAAGA GAGGCTTTGC ATCAAAGTCC TTCAGACACT CCGAGAGATG 
ATGACCAAAG ACAGAGGATA TGGAGAGAAG CTTCTGGGAT ATGATGATGA GATGGATGTC 
ACGGAGGTGG TGGATATCAG TCTGCCACCC AAACAGCAGG AGGATCGGCA GAGGGGGGAG 
GCCCTTCGGC AGCTGCTCGT CAATCGTTAC TATGGCTTCA GGAGTGGTGG GCGGAGAGAA 
AGTCTGACCT CGTTTGGTAA CTCCACCCTA ACTCCTGTCG GGCCAATCAA GAGCCAGCCA 
GGGGGGTTGA GCAGGGCTGA GATGTCATTA ATGGAGGTGC AGTGTCATCT AGACCGTGAA 
GGGGCATCGG ATCTTGTCAT TGATCTCATC ATGAACACTA CCAGCGACCG CGTCTTCCAT 
GAAAGCATTC TGTTGGCCAT CGCACTGTTG GAGGGCGGAA ACACCATCAT ACAGCATTCC 
TTCTTCAAAC GTTTAACAGA AGACAAAAAT TCAGAGAAGT TTTTCAGGGT GTTTTATGAC 
AGAATGAAAG TGGCTCAGGT GGAGATCAAA GCCACGGTTA CTGTCAACAC TAGTGACCTC 
GGCAACAAGC GGCGTGACGA CAACACTCCA GACAAAGATA CTCCACAACG CCGCAGAGGT 
AAAGACTCCG GTGTGGTGGT GACAGATGAT GCTCGTGAGC AGCTGTTGGA GGCTTCAGCT 
GCGACTAAGA AGGCCTTTGG TTCATATCGT CGAGATGCTG ATCCCGAGGA GACGTTTGGC 
ACGGCTGATG GAGATAAAGG AGGTGGAGAT AAAGGCACAG AGCAGGGCGA GATGAGCCCT 
GTCATCCTCA TAATGCAGCC AATCCTGCGC TTCCTGCAGC TGCTGTGCGA AAACCACAAT 
CGAGACCTGC AGAATTTTCT GCGCTGTCAG AACAATAAAA ACAACTATAA TCTGGTGTGT 
GAGACGCTGC AGTTCCTGGA CTGTATCTGT GGCAGCACAA CAGGAGGCCT GGGGCTGTTA 
GGACTCTACA TCAACCAGCA TAACGTAGCG CTCATTAATC AGACTGTAGA GAGCCTCACT 
GAATACTGTC AAGGTCCCTG CCATGACAAT CAGAATTGCA TTGCCACCCA TGAGTCTAAT 
GGCATCGACA TCATTATTGC ACTGATTCTG AATGATATTA ATCCACTGGG CAGGAAGAGA 
ATGGATCTGG TTCTAGAACT AAAGAATAAT GCATCTAAGC TGCTGCTGGC CATCATGGAG 
AGCCGTCATG ACAGTGAGAA TGCAGAAAGA ATTCTGTACA ACATGAGACC AAAAGAACTA 
GTGGAGGTGA TGAAAATGGC CTACCAGCAG GGGGAGGCAG AGTTTGAGGA TGAGGAACAG 
GAGAATGGAG AGGATCATGC AGCATCGCCT CGCAATGTTG GACACAACAT CTATATTCTT 
GCACATCAGC TGTCGCGTCA CAATAAGGAA TTGCAAATAC TACTGAAACC AGGTGGAGAG 
GACCAGGCCC TGGAGTATTA CACAAAACAC ACCTCACAGA TAGAGATAGT TCGACAGGAT 
CGCACTATGG AACAGATTGT GTTTCCTGTA CCAAATATCT GCTCTTTTCT GACAAATGAG 
TCCAAGCTGC GTGTGTACTA TGGTACAGAG AGAGACGAGC AAGGCAGTAA AATAAATGAC 
TTCTTCTTGC ATGCAGACGA CCTCTTTAAT GAAATGAGGT GGCAGAAAAA ACTAAGAGCT 
CAGCCAGTGC TGTACTGGTG CAGCAGGAAC ATGTCGTTTT GGAGTAACGT TTCCTTTAAT 
CTGGCGGTGC TCATTAATTT GCTGGTGGCA TTTTTCTACC CTCTGGATGG AGTGAGCGAA 
AGCCAGCTGG AACCTTCTCT GTCTCTGTTG CTGTGGGTTT GTTTGTTGGG CTCACTGGGC 
TTCGTGTTGA TGTCCCCGAG GCCAAATGCA GTGCGGGTGC TGGTCATCTC CACTGTCCTG 
CAGTTGGGCT TCTCTGTAGG GCTGCAGCAC ATGCTCACAC TCCTGGGAGC TTTCAATGTT 
TGTAATAAGA TCGTGTTCAT GCTGAGCTTT GTGGGTAACC GTGGGTCATT CACCCGGGGT 
TATCGGGTGA TGGTCATGGA TAGAGAGTTC CTGTTTCATC TGCTGTACCT TCTCATCTGC 
ATACTGGGCC TTTTTGGACA CGTCTTCTTT TACAGTCTTC TGTTGTTTGA CCTGGTGAAC 
CGGGAAGAGA CTCTTCTGAA CGTCATCAAG AGTGTGACTC GAAACGGCCG CTCCATCGTC 
TTGACCGCAG TGCTGGGCCT CATCCTGGTC TACCTCTTTT CTATTGTTGG ATATATGTTT 
TTCAAAGATG ACTTCATCCT GGAGGTGAAC CGCATCTCCA ACGGCACTCT GGAGGAGGGG 
GTGACTTTAC CTGCTGGTTT TTGTGATGCG AGAAACGGGT CGTGTCTTTC TGATGATGTT 
GACGAGGATG ACGTGGAGCG CGCGTGTGAT TCTCTATGGA TGTGTATGAT CACTGTACTC 
AGTCATGGCC TCAGGAGCGG TGGAGGAGTT GGAGATGTCC TGCGCAAACC GTCTAAAGAG 
GAGCGTCTGT TCGCCGCACG GGTGGTGTAC GATCTCCTGT TCTTCTTCCT GGTGATCATC 
ATTGTGTTGA ATCTGATCTT CGGTGTGATC ATTGACACAT TTGCAGATCT GAGAAGCGAG 
AAGCAGAGAA AAGAGGAGAT CTTGAAGACC ACATGCTTCA TCTGTGGTCT GGAGAGAGAT 
AAGTTTGATA ATAAAACGGT GACGTTTGAA GAGCACATCA AGGAGGAGCA CAACCTGTGG 
CATTACCTCT TCTTCATAGT GCTGGTGCGG GTCAAAGACT CCACCGAATA CACGGGACCC 
GAGAGCTACG TCGCTCAGAT GATCAAGGAG CACAATCTGG ACTGGTTTCC TCGTATGAGG 
GCGATGTCTC TGGTCAGCAG TGATGGAGAA GGTGAACAGA ATGAACTGCG CAGCTTACAG 
GAGAAACTCG AGTCCACAAT GAGGCTCGTC TCTAATCTTA CCAATCAGCT CACAGAGCTC 
AAAGAGCAGA TGACTGAGCA AAGGAAACAC AAGCAGAGGC TCGGTCTGCT GGGAAACCCC 
GCCCACCTCA ACATCAACCC ACAGCAGCCA GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330289.1
CDS	163..8298
Translation	MVDKLSSFLHIGDVCSLYAEGSTSGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGTSTTDTVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDAAGNEGSWFYIQPFYKLRSIGDNVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCTTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDEHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEVNPDYEEECLESRSSMDSEHEVIRARARNPQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSPLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAAKLEKGTITQNERRSVTKLLEDLVYFVVDIPSSAQDVLEITVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRYSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDPANADILIETKLVLSRFEVAGTVLGEGAEEEEEDEEEVWLFWKDSGGEVKSKSIRELAQDAKDGHTEDQEVINYYRYQLNLFARMCLDRQYLAINKISAQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSQISIDDYDNDGTSRDEVKERFSQTMEFVENYLRDVVCQSFPFSDKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHISTPFHIKLDREPGKGSNVMRSIHGVGELMTQVVLRGGGFLPASSHNPPDRDEVKAQSEPQKQDILVMDTKLKIIEILQFILNVRLDYRISCLLCIFKTEFDESNSQSEPSLSPESPASVQGALDFEHIEEQAEGIFGGSSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALHLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSDSGLDAGEVTPEAHHKGVINSSRSNKPKVESTSSSNYRVVKEILLRLSKLCVLEGISGKKNKKQQQRLLRNMGAHSVVLELLQIPYEKGEDVQMQEIMTLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAVTMQHIFMNNFQLCSEINERVVQHFVHCIETHGRSVHYLKFLQTIVKAENKFIKKCQDIVMAELVTAGEDVLVFYNDRASFQSLVQMMRLERERLDESSALRYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKMAYVNFLNHCYVDTEVEMKEIYTSNHMWKLFDDFLVDVCRVCNNTSDRKHADTVLERYVTETIMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQGVFRVYHCNWLLPGQKGNVEACIKVLSDVAKGRAIAIPVDLDCQVNNLFMKSNNIVQKTALSWRLSVRNATRRDSVLTTSRDYRNIIERLQDIVSALEERLRPLVQAELSVLVDVLHRPELLFPEHTEAHRKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKLLGYDDEMDVTEVVDISLPPKQQEDRQRGEALRQLLVNRYYGFRSGGRRESLTSFGNSTLTPVGPIKSQPGGLSRAEMSLMEVQCHLDREGASDLVIDLIMNTTSDRVFHESILLAIALLEGGNTIIQHSFFKRLTEDKNSEKFFRVFYDRMKVAQVEIKATVTVNTSDLGNKRRDDNTPDKDTPQRRRGKDSGVVVTDDAREQLLEASAATKKAFGSYRRDADPEETFGTADGDKGGGDKGTEQGEMSPVILIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINQHNVALINQTVESLTEYCQGPCHDNQNCIATHESNGIDIIIALILNDINPLGRKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVMKMAYQQGEAEFEDEEQENGEDHAASPRNVGHNIYILAHQLSRHNKELQILLKPGGEDQALEYYTKHTSQIEIVRQDRTMEQIVFPVPNICSFLTNESKLRVYYGTERDEQGSKINDFFLHADDLFNEMRWQKKLRAQPVLYWCSRNMSFWSNVSFNLAVLINLLVAFFYPLDGVSESQLEPSLSLLLWVCLLGSLGFVLMSPRPNAVRVLVISTVLQLGFSVGLQHMLTLLGAFNVCNKIVFMLSFVGNRGSFTRGYRVMVMDREFLFHLLYLLICILGLFGHVFFYSLLLFDLVNREETLLNVIKSVTRNGRSIVLTAVLGLILVYLFSIVGYMFFKDDFILEVNRISNGTLEEGVTLPAGFCDARNGSCLSDDVDEDDVERACDSLWMCMITVLSHGLRSGGGVGDVLRKPSKEERLFAARVVYDLLFFFLVIIIVLNLIFGVIIDTFADLRSEKQRKEEILKTTCFICGLERDKFDNKTVTFEEHIKEEHNLWHYLFFIVLVRVKDSTEYTGPESYVAQMIKEHNLDWFPRMRAMSLVSSDGEGEQNELRSLQEKLESTMRLVSNLTNQLTELKEQMTEQRKHKQRLGLLGNPAHLNINPQQPA

Target ORF information:

RefSeq Version	XM_021474614.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474614.1

ORF Insert Sequence:

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAATAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACT CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TAGAGGTGGT TCAGCATGAT CCCTGCCGAG GCGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GGTAAATCCA 
GACTATGAAG AAGAATGCCT GGAGTCCCGC TCCTCTATGG ATTCGGAGCA TGAAGTGATT 
CGGGCTCGTG CACGTAACCC TCAGGATAAG GTGATGTACA CACTGGTGTC TGTTCCTGAT 
GGAAATGACA TATCCTCCAT CTTTGAGCTT GACCCTACAA CTTTGAGGGG CGGAGACTCA 
CTTGTGCCAA GAAACTCGTA TGTGAGGCTC AGGCATCTCT GCACCAACAC GTGGGTCCAC 
AGCACCAACC AGCCAATAGA TAAAGAGGAA GAGAAACCGG TCATGCTCAG AATAGGAACG 
TCTCCTCTGA AGGAAGATAA AGAGGCGTTT GCTATAGTAC CAGTCTCTCC AGCTGAGGTT 
CGAGATCTGG ATTTTGCTAA TGATGCTAGT AAAGTGTTGG CCTCCATTGC TGCCAAACTG 
GAGAAGGGCA CCATCACCCA GAATGAGAGG AGGTCAGTGA CGAAGCTGTT GGAGGATCTG 
GTGTATTTTG TGGTGGACAT ACCCAGCAGT GCTCAAGATG TGCTGGAGAT CACAGTCAAC 
AAACCCAACA GAGAGCGACA GAAACTCATG AGAGAGCAGA ACATTCTTAA ACAGATCTTC 
AAGCTGCTGC AGGCGCCATT TACAGACAGC GGGGACGGCC CTATGTTGCG ATTAGAAGAG 
CTCGCAGACC AGCGTCATGC ACCCTTTCGA CACATTTGCA GACTCTGTTA CAGAGTCCTA 
CGTTACTCAC AACAGGACTA TCGCAAAAAT CAGGAGTACA TAGCAAAGCA GTTCCGCTTC 
ATGCAGAAGC AGATAGGATA TGATGTGCTG GCTGAAGACA CAATTACAGC CCTTCTGCAC 
AACAACAGGA AGCTCCTAGA GAAACACATC ACAGCAGCTG AAATAGATAC CTTTGTCAGT 
CTGGTCCGCA AGAACAGAGA GCCCAGATTT CTGGATTACT TGTCAGATCT GTGTGTGTCG 
ATGAATAAAT CCATCCCGGT CACTCAAGAG CTCATCTGTA ATGCTGTGCT GGACCCAGCC 
AATGCAGACA TTCTCATCGA AACCAAGCTT GTGTTGTCAC GATTTGAGGT GGCAGGAACG 
GTGTTGGGTG AAGGTGCGGA GGAGGAAGAG GAGGATGAAG AGGAGGTCTG GCTGTTCTGG 
AAGGACAGTG GAGGAGAGGT GAAGAGTAAA AGCATACGAG AGCTTGCACA GGACGCAAAA 
GATGGACATA CAGAGGACCA GGAAGTCATC AATTATTACA GATATCAGCT GAATTTGTTT 
GCTCGGATGT GTCTGGATCG ACAGTATCTT GCGATTAACA AGATATCAGC ACAGCTGGAT 
GTGGATCTGA TTCTGCGCTG CATGTCAGAT GAAGATTTGC CCTTTGACCT CAGAGCATCG 
TTCTGCCGCA TGATGCTGCA CATGCATGTA GACCGTGACC CTCAGGAGCA GGTCACCCCG 
GTCAAATATG CTCGACTGTG GTCTGAAATC CCATCACAAA TTTCCATTGA TGATTATGAT 
AATGATGGGA CGTCACGTGA TGAAGTGAAA GAGCGCTTCT CTCAGACGAT GGAGTTTGTG 
GAGAATTACC TGAGGGATGT TGTCTGCCAG AGTTTCCCGT TCTCTGATAA GGAGAAAAAC 
AAACTCACTT TTGAGGTGGT GAACCTGGCA AGAAACCTGA TCTATTTTGG TTTCTATAAC 
TTCAGTGATC TGCTAAGGTT AACAAAAATC CTATTGGCAA TCCTGGACTG TGTCCACATA 
AGCACTCCCT TCCACATCAA GCTGGATAGA GAACCTGGTA AAGGAAGTAA CGTGATGCGC 
TCCATCCATG GGGTGGGAGA ACTGATGACA CAGGTAGTGT TGAGGGGTGG GGGATTTCTG 
CCAGCCTCCA GCCACAATCC TCCTGACAGA GACGAAGTGA AAGCACAAAG TGAACCTCAG 
AAACAAGACA TACTGGTGAT GGACACTAAA CTCAAGATCA TCGAGATCCT GCAGTTTATT 
CTGAATGTGC GGTTGGACTA CAGGATCTCC TGCCTCCTCT GTATCTTTAA GACAGAGTTT 
GATGAAAGTA ACTCTCAAAG TGAACCATCT TTAAGCCCAG AATCTCCTGC CAGCGTTCAA 
GGTGCGCTAG ACTTTGAGCA CATTGAAGAG CAGGCGGAGG GTATTTTTGG AGGAAGTAGT 
GAAGAGAACA CCCCGCTGGA TCTTGATGAT CATGGTGGAC GGACATTTCT GAGGGTCCTG 
CTTCACCTGA CCATGCATGA CTATCCTCCG CTGGTATCTC GAGCTCTTCA CCTGCTCTTC 
AGACACTTTA GCCAACGCCA GGAGGTCCTA CAGGCCTTCA AACAGGTTCA GCTCCTGGTG 
ACCAGTCAGG ATGTGGAAAA CTATAAACAG ATCAAGTCAG ATTTGGACCA GCTGAGGTCA 
ATAGTAGAGA AATCAGAGTT GTGGGTGTAT AAGAGACAAG GCTCAGACTC AGGACTGGAT 
GCTGGAGAAG TCACCCCTGA AGCCCACCAC AAGGGTGTCA TCAACTCGAG TAGATCAAAC 
AAACCCAAAG TGGAAAGTAC CAGCAGCTCG AACTACAGAG TCGTGAAAGA GATCCTGCTG 
AGGCTGAGCA AACTGTGTGT TTTGGAGGGC ATCTCTGGCA AGAAGAATAA GAAACAGCAG 
CAGCGGCTCC TCAGGAACAT GGGAGCTCAC AGTGTGGTCC TCGAACTGTT GCAAATCCCT 
TATGAGAAGG GGGAGGATGT CCAGATGCAG GAGATCATGA CCTTAGCACA TCAGTTCCTT 
CAAAACTTCT GTGCTGGAAA TCAGCAGAAC CAGGCACTTC TACACAAACA CATCAACCTG 
TTCCTCAACC CTGGGATCCT GGAGGCGGTG ACCATGCAGC ATATCTTCAT GAATAATTTC 
CAGTTGTGTA GTGAGATTAA TGAGCGGGTC GTGCAGCATT TTGTGCACTG CATCGAGACA 
CACGGCCGCA GCGTGCACTA CCTCAAGTTC CTGCAGACCA TTGTCAAGGC AGAGAACAAG 
TTCATCAAGA AATGCCAAGA CATCGTCATG GCTGAGCTGG TGACTGCAGG TGAGGATGTG 
CTGGTGTTCT ATAATGACCG GGCGTCTTTT CAGTCATTGG TTCAGATGAT GCGTCTTGAG 
CGGGAACGAC TTGATGAGAG CAGTGCACTC AGGTATCACA TTCACCTGGT GGAGTTGCTG 
GCTGTGTGCA CTGAAGGCAA GAACGTCTAC ACTGAGATCA AATGCAACTC GCTGCTCCCT 
CTCGATGATA TTGTACGTGT GGTCACACAT GAGGACTGCA TCCCTGAGGT GAAGATGGCC 
TACGTGAACT TCCTGAATCA CTGTTATGTT GATACAGAGG TTGAGATGAA GGAGATCTAC 
ACCAGCAATC ACATGTGGAA ACTTTTTGAT GATTTTCTGG TGGACGTCTG CAGAGTGTGC 
AATAACACAA GTGACCGTAA ACACGCTGAC ACAGTTCTGG AGAGATACGT TACAGAGACC 
ATAATGAGCA TCGTCACCAC CTTTTTCAGC TCACCATTCT CAGACCAGAG CACAAGTTTG 
CAGACCCGGC AGCCGGTGTT TGTGCAGCTG CTGCAGGGAG TGTTTCGTGT ATATCACTGC 
AACTGGCTTC TCCCAGGTCA GAAGGGGAAT GTGGAAGCCT GCATCAAAGT GCTGAGTGAC 
GTGGCCAAAG GCAGAGCCAT AGCCATTCCT GTGGATCTGG ACTGTCAGGT GAATAATCTG 
TTCATGAAGT CCAACAACAT TGTGCAGAAA ACAGCCCTCA GCTGGAGACT CTCTGTCCGT 
AACGCGACTC GGAGAGACTC TGTTCTGACC ACCTCTCGAG ACTATCGCAA CATCATTGAA 
AGACTACAGG ATATAGTGTC TGCATTGGAG GAGCGGTTGA GGCCTCTGGT TCAGGCTGAG 
TTGTCAGTGT TGGTGGATGT TCTCCATCGC CCAGAGCTGC TGTTTCCAGA GCACACTGAA 
GCCCACCGCA AGTGTGAGAG TGGAGGCTTC ATCTGCAAGT TGATAAAACA CACTAAGCAG 
CTTCTAGAAG AAAACGAAGA GAGGCTTTGC ATCAAAGTCC TTCAGACACT CCGAGAGATG 
ATGACCAAAG ACAGAGGATA TGGAGAGAAG CTTCTGGGAT ATGATGATGA GATGGATGTC 
ACGGAGGTGG TGGATATCAG TCTGCCACCC AAACAGCAGG AGGATCGGCA GAGGGGGGAG 
GCCCTTCGGC AGCTGCTCGT CAATCGTTAC TATGGCTTCA GGAGTGGTGG GCGGAGAGAA 
AGTCTGACCT CGTTTGGTAA CTCCACCCTA ACTCCTGTCG GGCCAATCAA GAGCCAGCCA 
GGGGGGTTGA GCAGGGCTGA GATGTCATTA ATGGAGGTGC AGTGTCATCT AGACCGTGAA 
GGGGCATCGG ATCTTGTCAT TGATCTCATC ATGAACACTA CCAGCGACCG CGTCTTCCAT 
GAAAGCATTC TGTTGGCCAT CGCACTGTTG GAGGGCGGAA ACACCATCAT ACAGCATTCC 
TTCTTCAAAC GTTTAACAGA AGACAAAAAT TCAGAGAAGT TTTTCAGGGT GTTTTATGAC 
AGAATGAAAG TGGCTCAGGT GGAGATCAAA GCCACGGTTA CTGTCAACAC TAGTGACCTC 
GGCAACAAGC GGCGTGACGA CAACACTCCA GACAAAGATA CTCCACAACG CCGCAGAGGT 
AAAGACTCCG GTGTGGTGGT GACAGATGAT GCTCGTGAGC AGCTGTTGGA GGCTTCAGCT 
GCGACTAAGA AGGCCTTTGG TTCATATCGT CGAGATGCTG ATCCCGAGGA GACGTTTGGC 
ACGGCTGATG GAGATAAAGG AGGTGGAGAT AAAGGCACAG AGCAGGGCGA GATGAGCCCT 
GTCATCCTCA TAATGCAGCC AATCCTGCGC TTCCTGCAGC TGCTGTGCGA AAACCACAAT 
CGAGACCTGC AGAATTTTCT GCGCTGTCAG AACAATAAAA ACAACTATAA TCTGGTGTGT 
GAGACGCTGC AGTTCCTGGA CTGTATCTGT GGCAGCACAA CAGGAGGCCT GGGGCTGTTA 
GGACTCTACA TCAACCAGCA TAACGTAGCG CTCATTAATC AGACTGTAGA GAGCCTCACT 
GAATACTGTC AAGGTCCCTG CCATGACAAT CAGAATTGCA TTGCCACCCA TGAGTCTAAT 
GGCATCGACA TCATTATTGC ACTGATTCTG AATGATATTA ATCCACTGGG CAGGAAGAGA 
ATGGATCTGG TTCTAGAACT AAAGAATAAT GCATCTAAGC TGCTGCTGGC CATCATGGAG 
AGCCGTCATG ACAGTGAGAA TGCAGAAAGA ATTCTGTACA ACATGAGACC AAAAGAACTA 
GTGGAGGTGA TGAAAATGGC CTACCAGCAG GGGGAGGCAG AGTTTGAGGA TGAGGAACAG 
GAGAATGGAG AGGATCATGC AGCATCGCCT CGCAATGTTG GACACAACAT CTATATTCTT 
GCACATCAGC TGTCGCGTCA CAATAAGGAA TTGCAAATAC TACTGAAACC AGGTGGAGAG 
GACCAGGCCC TGGAGTATTA CACAAAACAC ACCTCACAGA TAGAGATAGT TCGACAGGAT 
CGCACTATGG AACAGATTGT GTTTCCTGTA CCAAATATCT GCTCTTTTCT GACAAATGAG 
TCCAAGCTGC GTGTGTACTA TGGTACAGAG AGAGACGAGC AAGGCAGTAA AATAAATGAC 
TTCTTCTTGC ATGCAGACGA CCTCTTTAAT GAAATGAGGT GGCAGAAAAA ACTAAGAGCT 
CAGCCAGTGC TGTACTGGTG CAGCAGGAAC ATGTCGTTTT GGAGTAACGT TTCCTTTAAT 
CTGGCGGTGC TCATTAATTT GCTGGTGGCA TTTTTCTACC CTCTGGATGG AGTGAGCGAA 
AGCCAGCTGG AACCTTCTCT GTCTCTGTTG CTGTGGGTTT GTTTGTTGGG CTCACTGGGC 
TTCGTGTTGA TGTCCCCGAG GCCAAATGCA GTGCGGGTGC TGGTCATCTC CACTGTCCTG 
CAGTTGGGCT TCTCTGTAGG GCTGCAGCAC ATGCTCACAC TCCTGGGAGC TTTCAATGTT 
TGTAATAAGA TCGTGTTCAT GCTGAGCTTT GTGGGTAACC GTGGGTCATT CACCCGGGGT 
TATCGGGTGA TGGTCATGGA TAGAGAGTTC CTGTTTCATC TGCTGTACCT TCTCATCTGC 
ATACTGGGCC TTTTTGGACA CGTCTTCTTT TACAGTCTTC TGTTGTTTGA CCTGGTGAAC 
CGGGAAGAGA CTCTTCTGAA CGTCATCAAG AGTGTGACTC GAAACGGCCG CTCCATCGTC 
TTGACCGCAG TGCTGGGCCT CATCCTGGTC TACCTCTTTT CTATTGTTGG ATATATGTTT 
TTCAAAGATG ACTTCATCCT GGAGGTGAAC CGCATCTCCA ACGGCACTCT GGAGGAGGGG 
GTGACTTTAC CTGCTGGTTT TTGTGATGCG AGAAACGGGT CGTGTCTTTC TGATGATGTT 
GACGAGGATG ACGTGGAGCG CGCGTGTGAT TCTCTATGGA TGTGTATGAT CACTGTACTC 
AGTCATGGCC TCAGGAGCGG TGGAGGAGTT GGAGATGTCC TGCGCAAACC GTCTAAAGAG 
GAGCGTCTGT TCGCCGCACG GGTGGTGTAC GATCTCCTGT TCTTCTTCCT GGTGATCATC 
ATTGTGTTGA ATCTGATCTT CGGTGTGATC ATTGACACAT TTGCAGATCT GAGAAGCGAG 
AAGCAGAGAA AAGAGGAGAT CTTGAAGACC ACATGCTTCA TCTGTGGTCT GGAGAGAGAT 
AAGTTTGATA ATAAAACGGT GACGTTTGAA GAGCACATCA AGGAGGAGCA CAACCTGTGG 
CATTACCTCT TCTTCATAGT GCTGGTGCGG GTCAAAGACT CCACCGAATA CACGGGACCC 
GAGAGCTACG TCGCTCAGAT GATCAAGGAG CACAATCTGG ACTGGTTTCC TCGTATGAGG 
GCGATGTCTC TGGTCAGCAG TGATGGAGAA GGTGAACAGA ATGAACTGCG CAGCTTACAG 
GAGAAACTCG AGTCCACAAT GAGGCTCGTC TCTAATCTTA CCAATCAGCT CACAGAGCTC 
AAAGAGCAGA TGACTGAGCA AAGGAAACAC AAGCAGAGGC TCGGTCTGCT GGGAAACCCC 
GCCCACCTCA ACATCAACCC ACAGCAGCCA GCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itpr1a ( XM_021474615.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330290.1 Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X11, mRNA.

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CloneID	ODa146874
Clone ID Related Accession (Same CDS sequence)	XM_021474615.1
Accession Version	XM_021474615.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8124bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395382.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACAT TACAAGGTGC TGGAGTTTTT GCCATGGTGG ACAAATTGTC TAGTTTCCTG 
CACATTGGGG ACGTTTGTTC CCTTTATGCT GAAGGCTCTA CTAGTGGCTT CATCAGCACT 
CTGGGGTTGG TTGATGACCG ATGTGTTGTT CAACCAGATG CCGGAGACCT GAATAACCCA 
CCTAAAAAGT TTCGGGACTG TCTGTTCAGA CTGTGTCCCA TGAACAGATA TTCGGCACAG 
AAACAGTTCT GGAAAGCTGC CAAACCTGGA GGAACTAGCA CCACAGATAC TGTCCTGCTT 
AACAAACTCC ATCATGCTGC TGATTTAGAG AAGAAACAAA ATGATTCTGA GAACAAAAAG 
CTGCTTGGAA CCGTCATTCA GTATGGCAAT GTCATCCAGC TTCTCCATCT GAAGAGTAAT 
AAATATCTGA CGGTGAATAA GCGTTTGCCG GCTCTTCTTG AGAAGAATGC CATGCGTGTG 
ACTCTGGACG CTGCAGGGAA TGAAGGCTCG TGGTTTTACA TCCAGCCCTT CTATAAGCTG 
CGCTCTATAG GGGATAATGT GGTCATTGGA GATAAGGTCG TGTTAAACCC TGTCAATGCC 
GGACAGCCGC TGCACGCCAG CAGCCATCAG CTTGTGGATA ATCCAGGCTG CAATGAAGTG 
AATTCAGTGA ACTGCACCAC CAGTTGGAAA ATCGTTCTCT TCATGAAATG GAGCGACAAC 
CAGGAGATTG TCTTGAAAGG AGGTGACGTG GTGAGGCTGT TTCATGCAGA GCAGGAGAAG 
TTTCTGACAT GTGATGAGCA TCGGAAAAAG CAGTATGTGT TTCTGCGTAC CACCGGACGC 
CAGTCTGCAA CCTCCGCCAC TAGTAGCAAA GCACTCTGGG AGGTAGAGGT GGTTCAGCAT 
GATCCCTGCC GAGGCGGAGC CGGCTACTGG AACAGCCTCT TTAGGTTTAA ACACTTAGCC 
ACCGGCCATT ACCTTGCTGC AGAGATGGAT TCGGAGCATG AAGTGATTCG GGCTCGTGCA 
CGTAACCCTC AGGATAAGGT GATGTACACA CTGGTGTCTG TTCCTGATGG AAATGACATA 
TCCTCCATCT TTGAGCTTGA CCCTACAACT TTGAGGGGCG GAGACTCACT TGTGCCAAGA 
AACTCGTATG TGAGGCTCAG GCATCTCTGC ACCAACACGT GGGTCCACAG CACCAACCAG 
CCAATAGATA AAGAGGAAGA GAAACCGGTC ATGCTCAGAA TAGGAACGTC TCCTCTGAAG 
GAAGATAAAG AGGCGTTTGC TATAGTACCA GTCTCTCCAG CTGAGGTTCG AGATCTGGAT 
TTTGCTAATG ATGCTAGTAA AGTGTTGGCC TCCATTGCTG CCAAACTGGA GAAGGGCACC 
ATCACCCAGA ATGAGAGGAG GTCAGTGACG AAGCTGTTGG AGGATCTGGT GTATTTTGTG 
GTGGACATAC CCAGCAGTGC TCAAGATGTG CTGGAGATCA CAGTCAACAA ACCCAACAGA 
GAGCGACAGA AACTCATGAG AGAGCAGAAC ATTCTTAAAC AGATCTTCAA GCTGCTGCAG 
GCGCCATTTA CAGACAGCGG GGACGGCCCT ATGTTGCGAT TAGAAGAGCT CGCAGACCAG 
CGTCATGCAC CCTTTCGACA CATTTGCAGA CTCTGTTACA GAGTCCTACG TTACTCACAA 
CAGGACTATC GCAAAAATCA GGAGTACATA GCAAAGCAGT TCCGCTTCAT GCAGAAGCAG 
ATAGGATATG ATGTGCTGGC TGAAGACACA ATTACAGCCC TTCTGCACAA CAACAGGAAG 
CTCCTAGAGA AACACATCAC AGCAGCTGAA ATAGATACCT TTGTCAGTCT GGTCCGCAAG 
AACAGAGAGC CCAGATTTCT GGATTACTTG TCAGATCTGT GTGTGTCGAT GAATAAATCC 
ATCCCGGTCA CTCAAGAGCT CATCTGTAAT GCTGTGCTGG ACCCAGCCAA TGCAGACATT 
CTCATCGAAA CCAAGCTTGT GTTGTCACGA TTTGAGGTGG CAGGAACGGT GTTGGGTGAA 
GGTGCGGAGG AGGAAGAGGA GGATGAAGAG GAGGTCTGGC TGTTCTGGAA GGACAGTGGA 
GGAGAGGTGA AGAGTAAAAG CATACGAGAG CTTGCACAGG ACGCAAAAGA TGGACATACA 
GAGGACCAGG AAGTCATCAA TTATTACAGA TATCAGCTGA ATTTGTTTGC TCGGATGTGT 
CTGGATCGAC AGTATCTTGC GATTAACAAG ATATCAGCAC AGCTGGATGT GGATCTGATT 
CTGCGCTGCA TGTCAGATGA AGATTTGCCC TTTGACCTCA GAGCATCGTT CTGCCGCATG 
ATGCTGCACA TGCATGTAGA CCGTGACCCT CAGGAGCAGG TCACCCCGGT CAAATATGCT 
CGACTGTGGT CTGAAATCCC ATCACAAATT TCCATTGATG ATTATGATAA TGATGGGACG 
TCACGTGATG AAGTGAAAGA GCGCTTCTCT CAGACGATGG AGTTTGTGGA GAATTACCTG 
AGGGATGTTG TCTGCCAGAG TTTCCCGTTC TCTGATAAGG AGAAAAACAA ACTCACTTTT 
GAGGTGGTGA ACCTGGCAAG AAACCTGATC TATTTTGGTT TCTATAACTT CAGTGATCTG 
CTAAGGTTAA CAAAAATCCT ATTGGCAATC CTGGACTGTG TCCACATAAG CACTCCCTTC 
CACATCAAGC TGGATAGAGA ACCTGGTAAA GGAAGTAACG TGATGCGCTC CATCCATGGG 
GTGGGAGAAC TGATGACACA GGTAGTGTTG AGGGGTGGGG GATTTCTGCC AGCCTCCAGC 
CACAATCCTC CTGACAGAGA CGAAGTGAAA GCACAAAGTG AACCTCAGAA ACAAGACATA 
CTGGTGATGG ACACTAAACT CAAGATCATC GAGATCCTGC AGTTTATTCT GAATGTGCGG 
TTGGACTACA GGATCTCCTG CCTCCTCTGT ATCTTTAAGA CAGAGTTTGA TGAAAGTAAC 
TCTCAAAGTG AACCATCTTT AAGCCCAGAA TCTCCTGCCA GCGTTCAAGG TGCGCTAGAC 
TTTGAGCACA TTGAAGAGCA GGCGGAGGGT ATTTTTGGAG GAAGTAGTGA AGAGAACACC 
CCGCTGGATC TTGATGATCA TGGTGGACGG ACATTTCTGA GGGTCCTGCT TCACCTGACC 
ATGCATGACT ATCCTCCGCT GGTATCTCGA GCTCTTCACC TGCTCTTCAG ACACTTTAGC 
CAACGCCAGG AGGTCCTACA GGCCTTCAAA CAGGTTCAGC TCCTGGTGAC CAGTCAGGAT 
GTGGAAAACT ATAAACAGAT CAAGTCAGAT TTGGACCAGC TGAGGTCAAT AGTAGAGAAA 
TCAGAGTTGT GGGTGTATAA GAGACAAGGC TCAGACTCAG GACTGGATGC TGGAGAAGTC 
ACCCCTGAAG CCCACCACAA GGGTGTCATC AACTCGAGTA GATCAAACAA ACCCAAAGTG 
GAAAGTACCA GCAGCTCGAA CTACAGAGTC GTGAAAGAGA TCCTGCTGAG GCTGAGCAAA 
CTGTGTGTTT TGGAGGGCAT CTCTGGCAAG AAGAATAAGA AACAGCAGCA GCGGCTCCTC 
AGGAACATGG GAGCTCACAG TGTGGTCCTC GAACTGTTGC AAATCCCTTA TGAGAAGGGG 
GAGGATGTCC AGATGCAGGA GATCATGACC TTAGCACATC AGTTCCTTCA AAACTTCTGT 
GCTGGAAATC AGCAGAACCA GGCACTTCTA CACAAACACA TCAACCTGTT CCTCAACCCT 
GGGATCCTGG AGGCGGTGAC CATGCAGCAT ATCTTCATGA ATAATTTCCA GTTGTGTAGT 
GAGATTAATG AGCGGGTCGT GCAGCATTTT GTGCACTGCA TCGAGACACA CGGCCGCAGC 
GTGCACTACC TCAAGTTCCT GCAGACCATT GTCAAGGCAG AGAACAAGTT CATCAAGAAA 
TGCCAAGACA TCGTCATGGC TGAGCTGGTG ACTGCAGGTG AGGATGTGCT GGTGTTCTAT 
AATGACCGGG CGTCTTTTCA GTCATTGGTT CAGATGATGC GTCTTGAGCG GGAACGACTT 
GATGAGAGCA GTGCACTCAG GTATCACATT CACCTGGTGG AGTTGCTGGC TGTGTGCACT 
GAAGGCAAGA ACGTCTACAC TGAGATCAAA TGCAACTCGC TGCTCCCTCT CGATGATATT 
GTACGTGTGG TCACACATGA GGACTGCATC CCTGAGGTGA AGATGGCCTA CGTGAACTTC 
CTGAATCACT GTTATGTTGA TACAGAGGTT GAGATGAAGG AGATCTACAC CAGCAATCAC 
ATGTGGAAAC TTTTTGATGA TTTTCTGGTG GACGTCTGCA GAGTGTGCAA TAACACAAGT 
GACCGTAAAC ACGCTGACAC AGTTCTGGAG AGATACGTTA CAGAGACCAT AATGAGCATC 
GTCACCACCT TTTTCAGCTC ACCATTCTCA GACCAGAGCA CAAGTTTGCA GACCCGGCAG 
CCGGTGTTTG TGCAGCTGCT GCAGGGAGTG TTTCGTGTAT ATCACTGCAA CTGGCTTCTC 
CCAGGTCAGA AGGGGAATGT GGAAGCCTGC ATCAAAGTGC TGAGTGACGT GGCCAAAGGC 
AGAGCCATAG CCATTCCTGT GGATCTGGAC TGTCAGGTGA ATAATCTGTT CATGAAGTCC 
AACAACATTG TGCAGAAAAC AGCCCTCAGC TGGAGACTCT CTGTCCGTAA CGCGACTCGG 
AGAGACTCTG TTCTGACCAC CTCTCGAGAC TATCGCAACA TCATTGAAAG ACTACAGGAT 
ATAGTGTCTG CATTGGAGGA GCGGTTGAGG CCTCTGGTTC AGGCTGAGTT GTCAGTGTTG 
GTGGATGTTC TCCATCGCCC AGAGCTGCTG TTTCCAGAGC ACACTGAAGC CCACCGCAAG 
TGTGAGAGTG GAGGCTTCAT CTGCAAGTTG ATAAAACACA CTAAGCAGCT TCTAGAAGAA 
AACGAAGAGA GGCTTTGCAT CAAAGTCCTT CAGACACTCC GAGAGATGAT GACCAAAGAC 
AGAGGATATG GAGAGAAGCT TCTGGGATAT GATGATGAGA TGGATGTCAC GGAGGTGGTG 
GATATCAGTC TGCCACCCAA ACAGCAGGAG GATCGGCAGA GGGGGGAGGC CCTTCGGCAG 
CTGCTCGTCA ATCGTTACTA TGGCTTCAGG AGTGGTGGGC GGAGAGAAAG TCTGACCTCG 
TTTGGTAACT CCACCCTAAC TCCTGTCGGG CCAATCAAGA GCCAGCCAGG GGGGTTGAGC 
AGGGCTGAGA TGTCATTAAT GGAGGTGCAG TGTCATCTAG ACCGTGAAGG GGCATCGGAT 
CTTGTCATTG ATCTCATCAT GAACACTACC AGCGACCGCG TCTTCCATGA AAGCATTCTG 
TTGGCCATCG CACTGTTGGA GGGCGGAAAC ACCATCATAC AGCATTCCTT CTTCAAACGT 
TTAACAGAAG ACAAAAATTC AGAGAAGTTT TTCAGGGTGT TTTATGACAG AATGAAAGTG 
GCTCAGGTGG AGATCAAAGC CACGGTTACT GTCAACACTA GTGACCTCGG CAACAAGCGG 
CGTGACGACA ACACTCCAGA CAAAGATACT CCACAACGCC GCAGAGGTAA AGACTCCGGT 
GTGGTGGTGA CAGATGATGC TCGTGAGCAG CTGTTGGAGG CTTCAGCTGC GACTAAGAAG 
GCCTTTGGTT CATATCGTCG AGATGCTGAT CCCGAGGAGA CGTTTGGCAC GGCTGATGGA 
GATAAAGGAG GTGGAGATAA AGGCACAGAG CAGGGCGAGA TGAGCCCTGT CATCCTCATA 
ATGCAGCCAA TCCTGCGCTT CCTGCAGCTG CTGTGCGAAA ACCACAATCG AGACCTGCAG 
AATTTTCTGC GCTGTCAGAA CAATAAAAAC AACTATAATC TGGTGTGTGA GACGCTGCAG 
TTCCTGGACT GTATCTGTGG CAGCACAACA GGAGGCCTGG GGCTGTTAGG ACTCTACATC 
AACCAGCATA ACGTAGCGCT CATTAATCAG ACTGTAGAGA GCCTCACTGA ATACTGTCAA 
GGTCCCTGCC ATGACAATCA GAATTGCATT GCCACCCATG AGTCTAATGG CATCGACATC 
ATTATTGCAC TGATTCTGAA TGATATTAAT CCACTGGGCA GGAAGAGAAT GGATCTGGTT 
CTAGAACTAA AGAATAATGC ATCTAAGCTG CTGCTGGCCA TCATGGAGAG CCGTCATGAC 
AGTGAGAATG CAGAAAGAAT TCTGTACAAC ATGAGACCAA AAGAACTAGT GGAGGTGATG 
AAAATGGCCT ACCAGCAGGG GGAGGCAGAG TTTGAGGATG AGGAACAGGA GAATGGAGAG 
GATCATGCAG CATCGCCTCG CAATGTTGGA CACAACATCT ATATTCTTGC ACATCAGCTG 
TCGCGTCACA ATAAGGAATT GCAAATACTA CTGAAACCAG GTGGAGAGGA CCAGGCCCTG 
GAGTATTACA CAAAACACAC CTCACAGATA GAGATAGTTC GACAGGATCG CACTATGGAA 
CAGATTGTGT TTCCTGTACC AAATATCTGC TCTTTTCTGA CAAATGAGTC CAAGCTGCGT 
GTGTACTATG GTACAGAGAG AGACGAGCAA GGCAGTAAAA TAAATGACTT CTTCTTGCAT 
GCAGACGACC TCTTTAATGA AATGAGGTGG CAGAAAAAAC TAAGAGCTCA GCCAGTGCTG 
TACTGGTGCA GCAGGAACAT GTCGTTTTGG AGTAACGTTT CCTTTAATCT GGCGGTGCTC 
ATTAATTTGC TGGTGGCATT TTTCTACCCT CTGGATGGAG TGAGCGAAAG CCAGCTGGAA 
CCTTCTCTGT CTCTGTTGCT GTGGGTTTGT TTGTTGGGCT CACTGGGCTT CGTGTTGATG 
TCCCCGAGGC CAAATGCAGT GCGGGTGCTG GTCATCTCCA CTGTCCTGCA GTTGGGCTTC 
TCTGTAGGGC TGCAGCACAT GCTCACACTC CTGGGAGCTT TCAATGTTTG TAATAAGATC 
GTGTTCATGC TGAGCTTTGT GGGTAACCGT GGGTCATTCA CCCGGGGTTA TCGGGTGATG 
GTCATGGATA GAGAGTTCCT GTTTCATCTG CTGTACCTTC TCATCTGCAT ACTGGGCCTT 
TTTGGACACG TCTTCTTTTA CAGTCTTCTG TTGTTTGACC TGGTGAACCG GGAAGAGACT 
CTTCTGAACG TCATCAAGAG TGTGACTCGA AACGGCCGCT CCATCGTCTT GACCGCAGTG 
CTGGGCCTCA TCCTGGTCTA CCTCTTTTCT ATTGTTGGAT ATATGTTTTT CAAAGATGAC 
TTCATCCTGG AGGTGAACCG CATCTCCAAC GGCACTCTGG AGGAGGGGGT GACTTTACCT 
GCTGGTTTTT GTGATGCGAG AAACGGGTCG TGTCTTTCTG ATGATGTTGA CGAGGATGAC 
GTGGAGCGCG CGTGTGATTC TCTATGGATG TGTATGATCA CTGTACTCAG TCATGGCCTC 
AGGAGCGGTG GAGGAGTTGG AGATGTCCTG CGCAAACCGT CTAAAGAGGA GCGTCTGTTC 
GCCGCACGGG TGGTGTACGA TCTCCTGTTC TTCTTCCTGG TGATCATCAT TGTGTTGAAT 
CTGATCTTCG GTGTGATCAT TGACACATTT GCAGATCTGA GAAGCGAGAA GCAGAGAAAA 
GAGGAGATCT TGAAGACCAC ATGCTTCATC TGTGGTCTGG AGAGAGATAA GTTTGATAAT 
AAAACGGTGA CGTTTGAAGA GCACATCAAG GAGGAGCACA ACCTGTGGCA TTACCTCTTC 
TTCATAGTGC TGGTGCGGGT CAAAGACTCC ACCGAATACA CGGGACCCGA GAGCTACGTC 
GCTCAGATGA TCAAGGAGCA CAATCTGGAC TGGTTTCCTC GTATGAGGGC GATGTCTCTG 
GTCAGCAGTG ATGGAGAAGG TGAACAGAAT GAACTGCGCA GCTTACAGGA GAAACTCGAG 
TCCACAATGA GGCTCGTCTC TAATCTTACC AATCAGCTCA CAGAGCTCAA AGAGCAGATG 
ACTGAGCAAA GGAAACACAA GCAGAGGCTC GGTCTGCTGG GAAACCCCGC CCACCTCAAC 
ATCAACCCAC AGCAGCCAGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330290.1
CDS	265..8388
Translation	MMTLQGAGVFAMVDKLSSFLHIGDVCSLYAEGSTSGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGTSTTDTVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDAAGNEGSWFYIQPFYKLRSIGDNVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCTTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDEHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEMDSEHEVIRARARNPQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSPLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAAKLEKGTITQNERRSVTKLLEDLVYFVVDIPSSAQDVLEITVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRYSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDPANADILIETKLVLSRFEVAGTVLGEGAEEEEEDEEEVWLFWKDSGGEVKSKSIRELAQDAKDGHTEDQEVINYYRYQLNLFARMCLDRQYLAINKISAQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSQISIDDYDNDGTSRDEVKERFSQTMEFVENYLRDVVCQSFPFSDKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHISTPFHIKLDREPGKGSNVMRSIHGVGELMTQVVLRGGGFLPASSHNPPDRDEVKAQSEPQKQDILVMDTKLKIIEILQFILNVRLDYRISCLLCIFKTEFDESNSQSEPSLSPESPASVQGALDFEHIEEQAEGIFGGSSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALHLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSDSGLDAGEVTPEAHHKGVINSSRSNKPKVESTSSSNYRVVKEILLRLSKLCVLEGISGKKNKKQQQRLLRNMGAHSVVLELLQIPYEKGEDVQMQEIMTLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAVTMQHIFMNNFQLCSEINERVVQHFVHCIETHGRSVHYLKFLQTIVKAENKFIKKCQDIVMAELVTAGEDVLVFYNDRASFQSLVQMMRLERERLDESSALRYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKMAYVNFLNHCYVDTEVEMKEIYTSNHMWKLFDDFLVDVCRVCNNTSDRKHADTVLERYVTETIMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQGVFRVYHCNWLLPGQKGNVEACIKVLSDVAKGRAIAIPVDLDCQVNNLFMKSNNIVQKTALSWRLSVRNATRRDSVLTTSRDYRNIIERLQDIVSALEERLRPLVQAELSVLVDVLHRPELLFPEHTEAHRKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKLLGYDDEMDVTEVVDISLPPKQQEDRQRGEALRQLLVNRYYGFRSGGRRESLTSFGNSTLTPVGPIKSQPGGLSRAEMSLMEVQCHLDREGASDLVIDLIMNTTSDRVFHESILLAIALLEGGNTIIQHSFFKRLTEDKNSEKFFRVFYDRMKVAQVEIKATVTVNTSDLGNKRRDDNTPDKDTPQRRRGKDSGVVVTDDAREQLLEASAATKKAFGSYRRDADPEETFGTADGDKGGGDKGTEQGEMSPVILIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINQHNVALINQTVESLTEYCQGPCHDNQNCIATHESNGIDIIIALILNDINPLGRKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVMKMAYQQGEAEFEDEEQENGEDHAASPRNVGHNIYILAHQLSRHNKELQILLKPGGEDQALEYYTKHTSQIEIVRQDRTMEQIVFPVPNICSFLTNESKLRVYYGTERDEQGSKINDFFLHADDLFNEMRWQKKLRAQPVLYWCSRNMSFWSNVSFNLAVLINLLVAFFYPLDGVSESQLEPSLSLLLWVCLLGSLGFVLMSPRPNAVRVLVISTVLQLGFSVGLQHMLTLLGAFNVCNKIVFMLSFVGNRGSFTRGYRVMVMDREFLFHLLYLLICILGLFGHVFFYSLLLFDLVNREETLLNVIKSVTRNGRSIVLTAVLGLILVYLFSIVGYMFFKDDFILEVNRISNGTLEEGVTLPAGFCDARNGSCLSDDVDEDDVERACDSLWMCMITVLSHGLRSGGGVGDVLRKPSKEERLFAARVVYDLLFFFLVIIIVLNLIFGVIIDTFADLRSEKQRKEEILKTTCFICGLERDKFDNKTVTFEEHIKEEHNLWHYLFFIVLVRVKDSTEYTGPESYVAQMIKEHNLDWFPRMRAMSLVSSDGEGEQNELRSLQEKLESTMRLVSNLTNQLTELKEQMTEQRKHKQRLGLLGNPAHLNINPQQPA

Target ORF information:

RefSeq Version	XM_021474615.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474615.1

ORF Insert Sequence:

ATGATGACAT TACAAGGTGC TGGAGTTTTT GCCATGGTGG ACAAATTGTC TAGTTTCCTG 
CACATTGGGG ACGTTTGTTC CCTTTATGCT GAAGGCTCTA CTAGTGGCTT CATCAGCACT 
CTGGGGTTGG TTGATGACCG ATGTGTTGTT CAACCAGATG CCGGAGACCT GAATAACCCA 
CCTAAAAAGT TTCGGGACTG TCTGTTCAGA CTGTGTCCCA TGAACAGATA TTCGGCACAG 
AAACAGTTCT GGAAAGCTGC CAAACCTGGA GGAACTAGCA CCACAGATAC TGTCCTGCTT 
AACAAACTCC ATCATGCTGC TGATTTAGAG AAGAAACAAA ATGATTCTGA GAACAAAAAG 
CTGCTTGGAA CCGTCATTCA GTATGGCAAT GTCATCCAGC TTCTCCATCT GAAGAGTAAT 
AAATATCTGA CGGTGAATAA GCGTTTGCCG GCTCTTCTTG AGAAGAATGC CATGCGTGTG 
ACTCTGGACG CTGCAGGGAA TGAAGGCTCG TGGTTTTACA TCCAGCCCTT CTATAAGCTG 
CGCTCTATAG GGGATAATGT GGTCATTGGA GATAAGGTCG TGTTAAACCC TGTCAATGCC 
GGACAGCCGC TGCACGCCAG CAGCCATCAG CTTGTGGATA ATCCAGGCTG CAATGAAGTG 
AATTCAGTGA ACTGCACCAC CAGTTGGAAA ATCGTTCTCT TCATGAAATG GAGCGACAAC 
CAGGAGATTG TCTTGAAAGG AGGTGACGTG GTGAGGCTGT TTCATGCAGA GCAGGAGAAG 
TTTCTGACAT GTGATGAGCA TCGGAAAAAG CAGTATGTGT TTCTGCGTAC CACCGGACGC 
CAGTCTGCAA CCTCCGCCAC TAGTAGCAAA GCACTCTGGG AGGTAGAGGT GGTTCAGCAT 
GATCCCTGCC GAGGCGGAGC CGGCTACTGG AACAGCCTCT TTAGGTTTAA ACACTTAGCC 
ACCGGCCATT ACCTTGCTGC AGAGATGGAT TCGGAGCATG AAGTGATTCG GGCTCGTGCA 
CGTAACCCTC AGGATAAGGT GATGTACACA CTGGTGTCTG TTCCTGATGG AAATGACATA 
TCCTCCATCT TTGAGCTTGA CCCTACAACT TTGAGGGGCG GAGACTCACT TGTGCCAAGA 
AACTCGTATG TGAGGCTCAG GCATCTCTGC ACCAACACGT GGGTCCACAG CACCAACCAG 
CCAATAGATA AAGAGGAAGA GAAACCGGTC ATGCTCAGAA TAGGAACGTC TCCTCTGAAG 
GAAGATAAAG AGGCGTTTGC TATAGTACCA GTCTCTCCAG CTGAGGTTCG AGATCTGGAT 
TTTGCTAATG ATGCTAGTAA AGTGTTGGCC TCCATTGCTG CCAAACTGGA GAAGGGCACC 
ATCACCCAGA ATGAGAGGAG GTCAGTGACG AAGCTGTTGG AGGATCTGGT GTATTTTGTG 
GTGGACATAC CCAGCAGTGC TCAAGATGTG CTGGAGATCA CAGTCAACAA ACCCAACAGA 
GAGCGACAGA AACTCATGAG AGAGCAGAAC ATTCTTAAAC AGATCTTCAA GCTGCTGCAG 
GCGCCATTTA CAGACAGCGG GGACGGCCCT ATGTTGCGAT TAGAAGAGCT CGCAGACCAG 
CGTCATGCAC CCTTTCGACA CATTTGCAGA CTCTGTTACA GAGTCCTACG TTACTCACAA 
CAGGACTATC GCAAAAATCA GGAGTACATA GCAAAGCAGT TCCGCTTCAT GCAGAAGCAG 
ATAGGATATG ATGTGCTGGC TGAAGACACA ATTACAGCCC TTCTGCACAA CAACAGGAAG 
CTCCTAGAGA AACACATCAC AGCAGCTGAA ATAGATACCT TTGTCAGTCT GGTCCGCAAG 
AACAGAGAGC CCAGATTTCT GGATTACTTG TCAGATCTGT GTGTGTCGAT GAATAAATCC 
ATCCCGGTCA CTCAAGAGCT CATCTGTAAT GCTGTGCTGG ACCCAGCCAA TGCAGACATT 
CTCATCGAAA CCAAGCTTGT GTTGTCACGA TTTGAGGTGG CAGGAACGGT GTTGGGTGAA 
GGTGCGGAGG AGGAAGAGGA GGATGAAGAG GAGGTCTGGC TGTTCTGGAA GGACAGTGGA 
GGAGAGGTGA AGAGTAAAAG CATACGAGAG CTTGCACAGG ACGCAAAAGA TGGACATACA 
GAGGACCAGG AAGTCATCAA TTATTACAGA TATCAGCTGA ATTTGTTTGC TCGGATGTGT 
CTGGATCGAC AGTATCTTGC GATTAACAAG ATATCAGCAC AGCTGGATGT GGATCTGATT 
CTGCGCTGCA TGTCAGATGA AGATTTGCCC TTTGACCTCA GAGCATCGTT CTGCCGCATG 
ATGCTGCACA TGCATGTAGA CCGTGACCCT CAGGAGCAGG TCACCCCGGT CAAATATGCT 
CGACTGTGGT CTGAAATCCC ATCACAAATT TCCATTGATG ATTATGATAA TGATGGGACG 
TCACGTGATG AAGTGAAAGA GCGCTTCTCT CAGACGATGG AGTTTGTGGA GAATTACCTG 
AGGGATGTTG TCTGCCAGAG TTTCCCGTTC TCTGATAAGG AGAAAAACAA ACTCACTTTT 
GAGGTGGTGA ACCTGGCAAG AAACCTGATC TATTTTGGTT TCTATAACTT CAGTGATCTG 
CTAAGGTTAA CAAAAATCCT ATTGGCAATC CTGGACTGTG TCCACATAAG CACTCCCTTC 
CACATCAAGC TGGATAGAGA ACCTGGTAAA GGAAGTAACG TGATGCGCTC CATCCATGGG 
GTGGGAGAAC TGATGACACA GGTAGTGTTG AGGGGTGGGG GATTTCTGCC AGCCTCCAGC 
CACAATCCTC CTGACAGAGA CGAAGTGAAA GCACAAAGTG AACCTCAGAA ACAAGACATA 
CTGGTGATGG ACACTAAACT CAAGATCATC GAGATCCTGC AGTTTATTCT GAATGTGCGG 
TTGGACTACA GGATCTCCTG CCTCCTCTGT ATCTTTAAGA CAGAGTTTGA TGAAAGTAAC 
TCTCAAAGTG AACCATCTTT AAGCCCAGAA TCTCCTGCCA GCGTTCAAGG TGCGCTAGAC 
TTTGAGCACA TTGAAGAGCA GGCGGAGGGT ATTTTTGGAG GAAGTAGTGA AGAGAACACC 
CCGCTGGATC TTGATGATCA TGGTGGACGG ACATTTCTGA GGGTCCTGCT TCACCTGACC 
ATGCATGACT ATCCTCCGCT GGTATCTCGA GCTCTTCACC TGCTCTTCAG ACACTTTAGC 
CAACGCCAGG AGGTCCTACA GGCCTTCAAA CAGGTTCAGC TCCTGGTGAC CAGTCAGGAT 
GTGGAAAACT ATAAACAGAT CAAGTCAGAT TTGGACCAGC TGAGGTCAAT AGTAGAGAAA 
TCAGAGTTGT GGGTGTATAA GAGACAAGGC TCAGACTCAG GACTGGATGC TGGAGAAGTC 
ACCCCTGAAG CCCACCACAA GGGTGTCATC AACTCGAGTA GATCAAACAA ACCCAAAGTG 
GAAAGTACCA GCAGCTCGAA CTACAGAGTC GTGAAAGAGA TCCTGCTGAG GCTGAGCAAA 
CTGTGTGTTT TGGAGGGCAT CTCTGGCAAG AAGAATAAGA AACAGCAGCA GCGGCTCCTC 
AGGAACATGG GAGCTCACAG TGTGGTCCTC GAACTGTTGC AAATCCCTTA TGAGAAGGGG 
GAGGATGTCC AGATGCAGGA GATCATGACC TTAGCACATC AGTTCCTTCA AAACTTCTGT 
GCTGGAAATC AGCAGAACCA GGCACTTCTA CACAAACACA TCAACCTGTT CCTCAACCCT 
GGGATCCTGG AGGCGGTGAC CATGCAGCAT ATCTTCATGA ATAATTTCCA GTTGTGTAGT 
GAGATTAATG AGCGGGTCGT GCAGCATTTT GTGCACTGCA TCGAGACACA CGGCCGCAGC 
GTGCACTACC TCAAGTTCCT GCAGACCATT GTCAAGGCAG AGAACAAGTT CATCAAGAAA 
TGCCAAGACA TCGTCATGGC TGAGCTGGTG ACTGCAGGTG AGGATGTGCT GGTGTTCTAT 
AATGACCGGG CGTCTTTTCA GTCATTGGTT CAGATGATGC GTCTTGAGCG GGAACGACTT 
GATGAGAGCA GTGCACTCAG GTATCACATT CACCTGGTGG AGTTGCTGGC TGTGTGCACT 
GAAGGCAAGA ACGTCTACAC TGAGATCAAA TGCAACTCGC TGCTCCCTCT CGATGATATT 
GTACGTGTGG TCACACATGA GGACTGCATC CCTGAGGTGA AGATGGCCTA CGTGAACTTC 
CTGAATCACT GTTATGTTGA TACAGAGGTT GAGATGAAGG AGATCTACAC CAGCAATCAC 
ATGTGGAAAC TTTTTGATGA TTTTCTGGTG GACGTCTGCA GAGTGTGCAA TAACACAAGT 
GACCGTAAAC ACGCTGACAC AGTTCTGGAG AGATACGTTA CAGAGACCAT AATGAGCATC 
GTCACCACCT TTTTCAGCTC ACCATTCTCA GACCAGAGCA CAAGTTTGCA GACCCGGCAG 
CCGGTGTTTG TGCAGCTGCT GCAGGGAGTG TTTCGTGTAT ATCACTGCAA CTGGCTTCTC 
CCAGGTCAGA AGGGGAATGT GGAAGCCTGC ATCAAAGTGC TGAGTGACGT GGCCAAAGGC 
AGAGCCATAG CCATTCCTGT GGATCTGGAC TGTCAGGTGA ATAATCTGTT CATGAAGTCC 
AACAACATTG TGCAGAAAAC AGCCCTCAGC TGGAGACTCT CTGTCCGTAA CGCGACTCGG 
AGAGACTCTG TTCTGACCAC CTCTCGAGAC TATCGCAACA TCATTGAAAG ACTACAGGAT 
ATAGTGTCTG CATTGGAGGA GCGGTTGAGG CCTCTGGTTC AGGCTGAGTT GTCAGTGTTG 
GTGGATGTTC TCCATCGCCC AGAGCTGCTG TTTCCAGAGC ACACTGAAGC CCACCGCAAG 
TGTGAGAGTG GAGGCTTCAT CTGCAAGTTG ATAAAACACA CTAAGCAGCT TCTAGAAGAA 
AACGAAGAGA GGCTTTGCAT CAAAGTCCTT CAGACACTCC GAGAGATGAT GACCAAAGAC 
AGAGGATATG GAGAGAAGCT TCTGGGATAT GATGATGAGA TGGATGTCAC GGAGGTGGTG 
GATATCAGTC TGCCACCCAA ACAGCAGGAG GATCGGCAGA GGGGGGAGGC CCTTCGGCAG 
CTGCTCGTCA ATCGTTACTA TGGCTTCAGG AGTGGTGGGC GGAGAGAAAG TCTGACCTCG 
TTTGGTAACT CCACCCTAAC TCCTGTCGGG CCAATCAAGA GCCAGCCAGG GGGGTTGAGC 
AGGGCTGAGA TGTCATTAAT GGAGGTGCAG TGTCATCTAG ACCGTGAAGG GGCATCGGAT 
CTTGTCATTG ATCTCATCAT GAACACTACC AGCGACCGCG TCTTCCATGA AAGCATTCTG 
TTGGCCATCG CACTGTTGGA GGGCGGAAAC ACCATCATAC AGCATTCCTT CTTCAAACGT 
TTAACAGAAG ACAAAAATTC AGAGAAGTTT TTCAGGGTGT TTTATGACAG AATGAAAGTG 
GCTCAGGTGG AGATCAAAGC CACGGTTACT GTCAACACTA GTGACCTCGG CAACAAGCGG 
CGTGACGACA ACACTCCAGA CAAAGATACT CCACAACGCC GCAGAGGTAA AGACTCCGGT 
GTGGTGGTGA CAGATGATGC TCGTGAGCAG CTGTTGGAGG CTTCAGCTGC GACTAAGAAG 
GCCTTTGGTT CATATCGTCG AGATGCTGAT CCCGAGGAGA CGTTTGGCAC GGCTGATGGA 
GATAAAGGAG GTGGAGATAA AGGCACAGAG CAGGGCGAGA TGAGCCCTGT CATCCTCATA 
ATGCAGCCAA TCCTGCGCTT CCTGCAGCTG CTGTGCGAAA ACCACAATCG AGACCTGCAG 
AATTTTCTGC GCTGTCAGAA CAATAAAAAC AACTATAATC TGGTGTGTGA GACGCTGCAG 
TTCCTGGACT GTATCTGTGG CAGCACAACA GGAGGCCTGG GGCTGTTAGG ACTCTACATC 
AACCAGCATA ACGTAGCGCT CATTAATCAG ACTGTAGAGA GCCTCACTGA ATACTGTCAA 
GGTCCCTGCC ATGACAATCA GAATTGCATT GCCACCCATG AGTCTAATGG CATCGACATC 
ATTATTGCAC TGATTCTGAA TGATATTAAT CCACTGGGCA GGAAGAGAAT GGATCTGGTT 
CTAGAACTAA AGAATAATGC ATCTAAGCTG CTGCTGGCCA TCATGGAGAG CCGTCATGAC 
AGTGAGAATG CAGAAAGAAT TCTGTACAAC ATGAGACCAA AAGAACTAGT GGAGGTGATG 
AAAATGGCCT ACCAGCAGGG GGAGGCAGAG TTTGAGGATG AGGAACAGGA GAATGGAGAG 
GATCATGCAG CATCGCCTCG CAATGTTGGA CACAACATCT ATATTCTTGC ACATCAGCTG 
TCGCGTCACA ATAAGGAATT GCAAATACTA CTGAAACCAG GTGGAGAGGA CCAGGCCCTG 
GAGTATTACA CAAAACACAC CTCACAGATA GAGATAGTTC GACAGGATCG CACTATGGAA 
CAGATTGTGT TTCCTGTACC AAATATCTGC TCTTTTCTGA CAAATGAGTC CAAGCTGCGT 
GTGTACTATG GTACAGAGAG AGACGAGCAA GGCAGTAAAA TAAATGACTT CTTCTTGCAT 
GCAGACGACC TCTTTAATGA AATGAGGTGG CAGAAAAAAC TAAGAGCTCA GCCAGTGCTG 
TACTGGTGCA GCAGGAACAT GTCGTTTTGG AGTAACGTTT CCTTTAATCT GGCGGTGCTC 
ATTAATTTGC TGGTGGCATT TTTCTACCCT CTGGATGGAG TGAGCGAAAG CCAGCTGGAA 
CCTTCTCTGT CTCTGTTGCT GTGGGTTTGT TTGTTGGGCT CACTGGGCTT CGTGTTGATG 
TCCCCGAGGC CAAATGCAGT GCGGGTGCTG GTCATCTCCA CTGTCCTGCA GTTGGGCTTC 
TCTGTAGGGC TGCAGCACAT GCTCACACTC CTGGGAGCTT TCAATGTTTG TAATAAGATC 
GTGTTCATGC TGAGCTTTGT GGGTAACCGT GGGTCATTCA CCCGGGGTTA TCGGGTGATG 
GTCATGGATA GAGAGTTCCT GTTTCATCTG CTGTACCTTC TCATCTGCAT ACTGGGCCTT 
TTTGGACACG TCTTCTTTTA CAGTCTTCTG TTGTTTGACC TGGTGAACCG GGAAGAGACT 
CTTCTGAACG TCATCAAGAG TGTGACTCGA AACGGCCGCT CCATCGTCTT GACCGCAGTG 
CTGGGCCTCA TCCTGGTCTA CCTCTTTTCT ATTGTTGGAT ATATGTTTTT CAAAGATGAC 
TTCATCCTGG AGGTGAACCG CATCTCCAAC GGCACTCTGG AGGAGGGGGT GACTTTACCT 
GCTGGTTTTT GTGATGCGAG AAACGGGTCG TGTCTTTCTG ATGATGTTGA CGAGGATGAC 
GTGGAGCGCG CGTGTGATTC TCTATGGATG TGTATGATCA CTGTACTCAG TCATGGCCTC 
AGGAGCGGTG GAGGAGTTGG AGATGTCCTG CGCAAACCGT CTAAAGAGGA GCGTCTGTTC 
GCCGCACGGG TGGTGTACGA TCTCCTGTTC TTCTTCCTGG TGATCATCAT TGTGTTGAAT 
CTGATCTTCG GTGTGATCAT TGACACATTT GCAGATCTGA GAAGCGAGAA GCAGAGAAAA 
GAGGAGATCT TGAAGACCAC ATGCTTCATC TGTGGTCTGG AGAGAGATAA GTTTGATAAT 
AAAACGGTGA CGTTTGAAGA GCACATCAAG GAGGAGCACA ACCTGTGGCA TTACCTCTTC 
TTCATAGTGC TGGTGCGGGT CAAAGACTCC ACCGAATACA CGGGACCCGA GAGCTACGTC 
GCTCAGATGA TCAAGGAGCA CAATCTGGAC TGGTTTCCTC GTATGAGGGC GATGTCTCTG 
GTCAGCAGTG ATGGAGAAGG TGAACAGAAT GAACTGCGCA GCTTACAGGA GAAACTCGAG 
TCCACAATGA GGCTCGTCTC TAATCTTACC AATCAGCTCA CAGAGCTCAA AGAGCAGATG 
ACTGAGCAAA GGAAACACAA GCAGAGGCTC GGTCTGCTGG GAAACCCCGC CCACCTCAAC 
ATCAACCCAC AGCAGCCAGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itpr1a ( XM_021474616.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330291.1 Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X12, mRNA.

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CloneID	ODa146875
Clone ID Related Accession (Same CDS sequence)	XM_021474616.1
Accession Version	XM_021474616.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8121bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395382.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACAT TACAAGGTGC TGGAGTTTTT GCCATGGTGG ACAAATTGTC TAGTTTCCTG 
CACATTGGGG ACGTTTGTTC CCTTTATGCT GAAGGCTCTA CTAGTGGCTT CATCAGCACT 
CTGGGGTTGG TTGATGACCG ATGTGTTGTT CAACCAGATG CCGGAGACCT GAATAACCCA 
CCTAAAAAGT TTCGGGACTG TCTGTTCAGA CTGTGTCCCA TGAACAGATA TTCGGCACAG 
AAACAGTTCT GGAAAGCTGC CAAACCTGGA GGAACTAGCA CCACAGATAC TGTCCTGCTT 
AACAAACTCC ATCATGCTGC TGATTTAGAG AAGAAACAAA ATGATTCTGA GAACAAAAAG 
CTGCTTGGAA CCGTCATTCA GTATGGCAAT GTCATCCAGC TTCTCCATCT GAAGAGTAAT 
AAATATCTGA CGGTGAATAA GCGTTTGCCG GCTCTTCTTG AGAAGAATGC CATGCGTGTG 
ACTCTGGACG CTGCAGGGAA TGAAGGCTCG TGGTTTTACA TCCAGCCCTT CTATAAGCTG 
CGCTCTATAG GGGATAATGT GGTCATTGGA GATAAGGTCG TGTTAAACCC TGTCAATGCC 
GGACAGCCGC TGCACGCCAG CAGCCATCAG CTTGTGGATA ATCCAGGCTG CAATGAAGTG 
AATTCAGTGA ACTGCACCAC CAGTTGGAAA ATCGTTCTCT TCATGAAATG GAGCGACAAC 
CAGGAGATTG TCTTGAAAGG AGGTGACGTG GTGAGGCTGT TTCATGCAGA GCAGGAGAAG 
TTTCTGACAT GTGATGAGCA TCGGAAAAAG CAGTATGTGT TTCTGCGTAC CACCGGACGC 
CAGTCTGCAA CCTCCGCCAC TAGTAGCAAA GCACTCTGGG AGGTAGAGGT GGTTCAGCAT 
GATCCCTGCC GAGGCGGAGC CGGCTACTGG AACAGCCTCT TTAGGTTTAA ACACTTAGCC 
ACCGGCCATT ACCTTGCTGC AGAGATGGAT TCGGAGCATG AAGTGATTCG GGCTCGTGCA 
CGTAACCCTC AGGATAAGGT GATGTACACA CTGGTGTCTG TTCCTGATGG AAATGACATA 
TCCTCCATCT TTGAGCTTGA CCCTACAACT TTGAGGGGCG GAGACTCACT TGTGCCAAGA 
AACTCGTATG TGAGGCTCAG GCATCTCTGC ACCAACACGT GGGTCCACAG CACCAACCAG 
CCAATAGATA AAGAGGAAGA GAAACCGGTC ATGCTCAGAA TAGGAACGTC TCCTCTGAAG 
GAAGATAAAG AGGCGTTTGC TATAGTACCA GTCTCTCCAG CTGAGGTTCG AGATCTGGAT 
TTTGCTAATG ATGCTAGTAA AGTGTTGGCC TCCATTGCTG CCAAACTGGA GAAGGGCACC 
ATCACCCAGA ATGAGAGGAG GTCAGTGACG AAGCTGTTGG AGGATCTGGT GTATTTTGTG 
GTGGACATAC CCAGCAGTGC TCAAGATGTG CTGGAGATCA CAGTCAACAA ACCCAACAGA 
GAGCGACAGA AACTCATGAG AGAGCAGAAC ATTCTTAAAC AGATCTTCAA GCTGCTGCAG 
GCGCCATTTA CAGACAGCGG GGACGGCCCT ATGTTGCGAT TAGAAGAGCT CGCAGACCAG 
CGTCATGCAC CCTTTCGACA CATTTGCAGA CTCTGTTACA GAGTCCTACG TTACTCACAA 
CAGGACTATC GCAAAAATCA GGAGTACATA GCAAAGCAGT TCCGCTTCAT GCAGAAGCAG 
ATAGGATATG ATGTGCTGGC TGAAGACACA ATTACAGCCC TTCTGCACAA CAACAGGAAG 
CTCCTAGAGA AACACATCAC AGCAGCTGAA ATAGATACCT TTGTCAGTCT GGTCCGCAAG 
AACAGAGAGC CCAGATTTCT GGATTACTTG TCAGATCTGT GTGTGTCGAT GAATAAATCC 
ATCCCGGTCA CTCAAGAGCT CATCTGTAAT GCTGTGCTGG ACCCAGCCAA TGCAGACATT 
CTCATCGAAA CCAAGCTTGT GTTGTCACGA TTTGAGGTGG CAGGAACGGT GTTGGGTGAA 
GGTGCGGAGG AGGAAGAGGA GGATGAAGAG GAGGTCTGGC TGTTCTGGAA GGACAGTGGA 
GGAGAGGTGA AGAGTAAAAG CATACGAGAG CTTGCACAGG ACGCAAAAGA TGGACATACA 
GAGGACCAGG AAGTCATCAA TTATTACAGA TATCAGCTGA ATTTGTTTGC TCGGATGTGT 
CTGGATCGAC AGTATCTTGC GATTAACAAG ATATCAGCAC AGCTGGATGT GGATCTGATT 
CTGCGCTGCA TGTCAGATGA AGATTTGCCC TTTGACCTCA GAGCATCGTT CTGCCGCATG 
ATGCTGCACA TGCATGTAGA CCGTGACCCT CAGGAGCAGG TCACCCCGGT CAAATATGCT 
CGACTGTGGT CTGAAATCCC ATCACAAATT TCCATTGATG ATTATGATAA TGATGGGACG 
TCACGTGATG AAGTGAAAGA GCGCTTCTCT CAGACGATGG AGTTTGTGGA GAATTACCTG 
AGGGATGTTG TCTGCCAGAG TTTCCCGTTC TCTGATAAGG AGAAAAACAA ACTCACTTTT 
GAGGTGGTGA ACCTGGCAAG AAACCTGATC TATTTTGGTT TCTATAACTT CAGTGATCTG 
CTAAGGTTAA CAAAAATCCT ATTGGCAATC CTGGACTGTG TCCACATAAG CACTCCCTTC 
CACATCAAGC TGGATAGAGA ACCTGGTAAA GGAAGTAACG TGATGCGCTC CATCCATGGG 
GTGGGAGAAC TGATGACACA GGTAGTGTTG AGGGGTGGGG GATTTCTGCC AGCCTCCAGC 
CACAATCCTC CTGACAGAGA CGAAGTGAAA GCACAAAGTG AACCTCAGAA ACAAGACATA 
CTGGTGATGG ACACTAAACT CAAGATCATC GAGATCCTGC AGTTTATTCT GAATGTGCGG 
TTGGACTACA GGATCTCCTG CCTCCTCTGT ATCTTTAAGA CAGAGTTTGA TGAAAGTAAC 
TCTCAAAGTG AACCATCTTT AAGCCCAGAA TCTCCTGCCA GCGTTCAAGG TGCGCTAGAC 
TTTGAGCACA TTGAAGAGCA GGCGGAGGGT ATTTTTGGAG GAAGTGAAGA GAACACCCCG 
CTGGATCTTG ATGATCATGG TGGACGGACA TTTCTGAGGG TCCTGCTTCA CCTGACCATG 
CATGACTATC CTCCGCTGGT ATCTCGAGCT CTTCACCTGC TCTTCAGACA CTTTAGCCAA 
CGCCAGGAGG TCCTACAGGC CTTCAAACAG GTTCAGCTCC TGGTGACCAG TCAGGATGTG 
GAAAACTATA AACAGATCAA GTCAGATTTG GACCAGCTGA GGTCAATAGT AGAGAAATCA 
GAGTTGTGGG TGTATAAGAG ACAAGGCTCA GACTCAGGAC TGGATGCTGG AGAAGTCACC 
CCTGAAGCCC ACCACAAGGG TGTCATCAAC TCGAGTAGAT CAAACAAACC CAAAGTGGAA 
AGTACCAGCA GCTCGAACTA CAGAGTCGTG AAAGAGATCC TGCTGAGGCT GAGCAAACTG 
TGTGTTTTGG AGGGCATCTC TGGCAAGAAG AATAAGAAAC AGCAGCAGCG GCTCCTCAGG 
AACATGGGAG CTCACAGTGT GGTCCTCGAA CTGTTGCAAA TCCCTTATGA GAAGGGGGAG 
GATGTCCAGA TGCAGGAGAT CATGACCTTA GCACATCAGT TCCTTCAAAA CTTCTGTGCT 
GGAAATCAGC AGAACCAGGC ACTTCTACAC AAACACATCA ACCTGTTCCT CAACCCTGGG 
ATCCTGGAGG CGGTGACCAT GCAGCATATC TTCATGAATA ATTTCCAGTT GTGTAGTGAG 
ATTAATGAGC GGGTCGTGCA GCATTTTGTG CACTGCATCG AGACACACGG CCGCAGCGTG 
CACTACCTCA AGTTCCTGCA GACCATTGTC AAGGCAGAGA ACAAGTTCAT CAAGAAATGC 
CAAGACATCG TCATGGCTGA GCTGGTGACT GCAGGTGAGG ATGTGCTGGT GTTCTATAAT 
GACCGGGCGT CTTTTCAGTC ATTGGTTCAG ATGATGCGTC TTGAGCGGGA ACGACTTGAT 
GAGAGCAGTG CACTCAGGTA TCACATTCAC CTGGTGGAGT TGCTGGCTGT GTGCACTGAA 
GGCAAGAACG TCTACACTGA GATCAAATGC AACTCGCTGC TCCCTCTCGA TGATATTGTA 
CGTGTGGTCA CACATGAGGA CTGCATCCCT GAGGTGAAGA TGGCCTACGT GAACTTCCTG 
AATCACTGTT ATGTTGATAC AGAGGTTGAG ATGAAGGAGA TCTACACCAG CAATCACATG 
TGGAAACTTT TTGATGATTT TCTGGTGGAC GTCTGCAGAG TGTGCAATAA CACAAGTGAC 
CGTAAACACG CTGACACAGT TCTGGAGAGA TACGTTACAG AGACCATAAT GAGCATCGTC 
ACCACCTTTT TCAGCTCACC ATTCTCAGAC CAGAGCACAA GTTTGCAGAC CCGGCAGCCG 
GTGTTTGTGC AGCTGCTGCA GGGAGTGTTT CGTGTATATC ACTGCAACTG GCTTCTCCCA 
GGTCAGAAGG GGAATGTGGA AGCCTGCATC AAAGTGCTGA GTGACGTGGC CAAAGGCAGA 
GCCATAGCCA TTCCTGTGGA TCTGGACTGT CAGGTGAATA ATCTGTTCAT GAAGTCCAAC 
AACATTGTGC AGAAAACAGC CCTCAGCTGG AGACTCTCTG TCCGTAACGC GACTCGGAGA 
GACTCTGTTC TGACCACCTC TCGAGACTAT CGCAACATCA TTGAAAGACT ACAGGATATA 
GTGTCTGCAT TGGAGGAGCG GTTGAGGCCT CTGGTTCAGG CTGAGTTGTC AGTGTTGGTG 
GATGTTCTCC ATCGCCCAGA GCTGCTGTTT CCAGAGCACA CTGAAGCCCA CCGCAAGTGT 
GAGAGTGGAG GCTTCATCTG CAAGTTGATA AAACACACTA AGCAGCTTCT AGAAGAAAAC 
GAAGAGAGGC TTTGCATCAA AGTCCTTCAG ACACTCCGAG AGATGATGAC CAAAGACAGA 
GGATATGGAG AGAAGCTTCT GGGATATGAT GATGAGATGG ATGTCACGGA GGTGGTGGAT 
ATCAGTCTGC CACCCAAACA GCAGGAGGAT CGGCAGAGGG GGGAGGCCCT TCGGCAGCTG 
CTCGTCAATC GTTACTATGG CTTCAGGAGT GGTGGGCGGA GAGAAAGTCT GACCTCGTTT 
GGTAACTCCA CCCTAACTCC TGTCGGGCCA ATCAAGAGCC AGCCAGGGGG GTTGAGCAGG 
GCTGAGATGT CATTAATGGA GGTGCAGTGT CATCTAGACC GTGAAGGGGC ATCGGATCTT 
GTCATTGATC TCATCATGAA CACTACCAGC GACCGCGTCT TCCATGAAAG CATTCTGTTG 
GCCATCGCAC TGTTGGAGGG CGGAAACACC ATCATACAGC ATTCCTTCTT CAAACGTTTA 
ACAGAAGACA AAAATTCAGA GAAGTTTTTC AGGGTGTTTT ATGACAGAAT GAAAGTGGCT 
CAGGTGGAGA TCAAAGCCAC GGTTACTGTC AACACTAGTG ACCTCGGCAA CAAGCGGCGT 
GACGACAACA CTCCAGACAA AGATACTCCA CAACGCCGCA GAGGTAAAGA CTCCGGTGTG 
GTGGTGACAG ATGATGCTCG TGAGCAGCTG TTGGAGGCTT CAGCTGCGAC TAAGAAGGCC 
TTTGGTTCAT ATCGTCGAGA TGCTGATCCC GAGGAGACGT TTGGCACGGC TGATGGAGAT 
AAAGGAGGTG GAGATAAAGG CACAGAGCAG GGCGAGATGA GCCCTGTCAT CCTCATAATG 
CAGCCAATCC TGCGCTTCCT GCAGCTGCTG TGCGAAAACC ACAATCGAGA CCTGCAGAAT 
TTTCTGCGCT GTCAGAACAA TAAAAACAAC TATAATCTGG TGTGTGAGAC GCTGCAGTTC 
CTGGACTGTA TCTGTGGCAG CACAACAGGA GGCCTGGGGC TGTTAGGACT CTACATCAAC 
CAGCATAACG TAGCGCTCAT TAATCAGACT GTAGAGAGCC TCACTGAATA CTGTCAAGGT 
CCCTGCCATG ACAATCAGAA TTGCATTGCC ACCCATGAGT CTAATGGCAT CGACATCATT 
ATTGCACTGA TTCTGAATGA TATTAATCCA CTGGGCAGGA AGAGAATGGA TCTGGTTCTA 
GAACTAAAGA ATAATGCATC TAAGCTGCTG CTGGCCATCA TGGAGAGCCG TCATGACAGT 
GAGAATGCAG AAAGAATTCT GTACAACATG AGACCAAAAG AACTAGTGGA GGTGATGAAA 
ATGGCCTACC AGCAGGGGGA GGCAGAGTTT GAGGATGAGG AACAGGAGAA TGGAGAGGAT 
CATGCAGCAT CGCCTCGCAA TGTTGGACAC AACATCTATA TTCTTGCACA TCAGCTGTCG 
CGTCACAATA AGGAATTGCA AATACTACTG AAACCAGGTG GAGAGGACCA GGCCCTGGAG 
TATTACACAA AACACACCTC ACAGATAGAG ATAGTTCGAC AGGATCGCAC TATGGAACAG 
ATTGTGTTTC CTGTACCAAA TATCTGCTCT TTTCTGACAA ATGAGTCCAA GCTGCGTGTG 
TACTATGGTA CAGAGAGAGA CGAGCAAGGC AGTAAAATAA ATGACTTCTT CTTGCATGCA 
GACGACCTCT TTAATGAAAT GAGGTGGCAG AAAAAACTAA GAGCTCAGCC AGTGCTGTAC 
TGGTGCAGCA GGAACATGTC GTTTTGGAGT AACGTTTCCT TTAATCTGGC GGTGCTCATT 
AATTTGCTGG TGGCATTTTT CTACCCTCTG GATGGAGTGA GCGAAAGCCA GCTGGAACCT 
TCTCTGTCTC TGTTGCTGTG GGTTTGTTTG TTGGGCTCAC TGGGCTTCGT GTTGATGTCC 
CCGAGGCCAA ATGCAGTGCG GGTGCTGGTC ATCTCCACTG TCCTGCAGTT GGGCTTCTCT 
GTAGGGCTGC AGCACATGCT CACACTCCTG GGAGCTTTCA ATGTTTGTAA TAAGATCGTG 
TTCATGCTGA GCTTTGTGGG TAACCGTGGG TCATTCACCC GGGGTTATCG GGTGATGGTC 
ATGGATAGAG AGTTCCTGTT TCATCTGCTG TACCTTCTCA TCTGCATACT GGGCCTTTTT 
GGACACGTCT TCTTTTACAG TCTTCTGTTG TTTGACCTGG TGAACCGGGA AGAGACTCTT 
CTGAACGTCA TCAAGAGTGT GACTCGAAAC GGCCGCTCCA TCGTCTTGAC CGCAGTGCTG 
GGCCTCATCC TGGTCTACCT CTTTTCTATT GTTGGATATA TGTTTTTCAA AGATGACTTC 
ATCCTGGAGG TGAACCGCAT CTCCAACGGC ACTCTGGAGG AGGGGGTGAC TTTACCTGCT 
GGTTTTTGTG ATGCGAGAAA CGGGTCGTGT CTTTCTGATG ATGTTGACGA GGATGACGTG 
GAGCGCGCGT GTGATTCTCT ATGGATGTGT ATGATCACTG TACTCAGTCA TGGCCTCAGG 
AGCGGTGGAG GAGTTGGAGA TGTCCTGCGC AAACCGTCTA AAGAGGAGCG TCTGTTCGCC 
GCACGGGTGG TGTACGATCT CCTGTTCTTC TTCCTGGTGA TCATCATTGT GTTGAATCTG 
ATCTTCGGTG TGATCATTGA CACATTTGCA GATCTGAGAA GCGAGAAGCA GAGAAAAGAG 
GAGATCTTGA AGACCACATG CTTCATCTGT GGTCTGGAGA GAGATAAGTT TGATAATAAA 
ACGGTGACGT TTGAAGAGCA CATCAAGGAG GAGCACAACC TGTGGCATTA CCTCTTCTTC 
ATAGTGCTGG TGCGGGTCAA AGACTCCACC GAATACACGG GACCCGAGAG CTACGTCGCT 
CAGATGATCA AGGAGCACAA TCTGGACTGG TTTCCTCGTA TGAGGGCGAT GTCTCTGGTC 
AGCAGTGATG GAGAAGGTGA ACAGAATGAA CTGCGCAGCT TACAGGAGAA ACTCGAGTCC 
ACAATGAGGC TCGTCTCTAA TCTTACCAAT CAGCTCACAG AGCTCAAAGA GCAGATGACT 
GAGCAAAGGA AACACAAGCA GAGGCTCGGT CTGCTGGGAA ACCCCGCCCA CCTCAACATC 
AACCCACAGC AGCCAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330291.1
CDS	265..8385
Translation	MMTLQGAGVFAMVDKLSSFLHIGDVCSLYAEGSTSGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGTSTTDTVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDAAGNEGSWFYIQPFYKLRSIGDNVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCTTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDEHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEMDSEHEVIRARARNPQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSPLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAAKLEKGTITQNERRSVTKLLEDLVYFVVDIPSSAQDVLEITVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRYSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDPANADILIETKLVLSRFEVAGTVLGEGAEEEEEDEEEVWLFWKDSGGEVKSKSIRELAQDAKDGHTEDQEVINYYRYQLNLFARMCLDRQYLAINKISAQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSQISIDDYDNDGTSRDEVKERFSQTMEFVENYLRDVVCQSFPFSDKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHISTPFHIKLDREPGKGSNVMRSIHGVGELMTQVVLRGGGFLPASSHNPPDRDEVKAQSEPQKQDILVMDTKLKIIEILQFILNVRLDYRISCLLCIFKTEFDESNSQSEPSLSPESPASVQGALDFEHIEEQAEGIFGGSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALHLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSDSGLDAGEVTPEAHHKGVINSSRSNKPKVESTSSSNYRVVKEILLRLSKLCVLEGISGKKNKKQQQRLLRNMGAHSVVLELLQIPYEKGEDVQMQEIMTLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAVTMQHIFMNNFQLCSEINERVVQHFVHCIETHGRSVHYLKFLQTIVKAENKFIKKCQDIVMAELVTAGEDVLVFYNDRASFQSLVQMMRLERERLDESSALRYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKMAYVNFLNHCYVDTEVEMKEIYTSNHMWKLFDDFLVDVCRVCNNTSDRKHADTVLERYVTETIMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQGVFRVYHCNWLLPGQKGNVEACIKVLSDVAKGRAIAIPVDLDCQVNNLFMKSNNIVQKTALSWRLSVRNATRRDSVLTTSRDYRNIIERLQDIVSALEERLRPLVQAELSVLVDVLHRPELLFPEHTEAHRKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKLLGYDDEMDVTEVVDISLPPKQQEDRQRGEALRQLLVNRYYGFRSGGRRESLTSFGNSTLTPVGPIKSQPGGLSRAEMSLMEVQCHLDREGASDLVIDLIMNTTSDRVFHESILLAIALLEGGNTIIQHSFFKRLTEDKNSEKFFRVFYDRMKVAQVEIKATVTVNTSDLGNKRRDDNTPDKDTPQRRRGKDSGVVVTDDAREQLLEASAATKKAFGSYRRDADPEETFGTADGDKGGGDKGTEQGEMSPVILIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINQHNVALINQTVESLTEYCQGPCHDNQNCIATHESNGIDIIIALILNDINPLGRKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVMKMAYQQGEAEFEDEEQENGEDHAASPRNVGHNIYILAHQLSRHNKELQILLKPGGEDQALEYYTKHTSQIEIVRQDRTMEQIVFPVPNICSFLTNESKLRVYYGTERDEQGSKINDFFLHADDLFNEMRWQKKLRAQPVLYWCSRNMSFWSNVSFNLAVLINLLVAFFYPLDGVSESQLEPSLSLLLWVCLLGSLGFVLMSPRPNAVRVLVISTVLQLGFSVGLQHMLTLLGAFNVCNKIVFMLSFVGNRGSFTRGYRVMVMDREFLFHLLYLLICILGLFGHVFFYSLLLFDLVNREETLLNVIKSVTRNGRSIVLTAVLGLILVYLFSIVGYMFFKDDFILEVNRISNGTLEEGVTLPAGFCDARNGSCLSDDVDEDDVERACDSLWMCMITVLSHGLRSGGGVGDVLRKPSKEERLFAARVVYDLLFFFLVIIIVLNLIFGVIIDTFADLRSEKQRKEEILKTTCFICGLERDKFDNKTVTFEEHIKEEHNLWHYLFFIVLVRVKDSTEYTGPESYVAQMIKEHNLDWFPRMRAMSLVSSDGEGEQNELRSLQEKLESTMRLVSNLTNQLTELKEQMTEQRKHKQRLGLLGNPAHLNINPQQPA

Target ORF information:

RefSeq Version	XM_021474616.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474616.1

ORF Insert Sequence:

ATGATGACAT TACAAGGTGC TGGAGTTTTT GCCATGGTGG ACAAATTGTC TAGTTTCCTG 
CACATTGGGG ACGTTTGTTC CCTTTATGCT GAAGGCTCTA CTAGTGGCTT CATCAGCACT 
CTGGGGTTGG TTGATGACCG ATGTGTTGTT CAACCAGATG CCGGAGACCT GAATAACCCA 
CCTAAAAAGT TTCGGGACTG TCTGTTCAGA CTGTGTCCCA TGAACAGATA TTCGGCACAG 
AAACAGTTCT GGAAAGCTGC CAAACCTGGA GGAACTAGCA CCACAGATAC TGTCCTGCTT 
AACAAACTCC ATCATGCTGC TGATTTAGAG AAGAAACAAA ATGATTCTGA GAACAAAAAG 
CTGCTTGGAA CCGTCATTCA GTATGGCAAT GTCATCCAGC TTCTCCATCT GAAGAGTAAT 
AAATATCTGA CGGTGAATAA GCGTTTGCCG GCTCTTCTTG AGAAGAATGC CATGCGTGTG 
ACTCTGGACG CTGCAGGGAA TGAAGGCTCG TGGTTTTACA TCCAGCCCTT CTATAAGCTG 
CGCTCTATAG GGGATAATGT GGTCATTGGA GATAAGGTCG TGTTAAACCC TGTCAATGCC 
GGACAGCCGC TGCACGCCAG CAGCCATCAG CTTGTGGATA ATCCAGGCTG CAATGAAGTG 
AATTCAGTGA ACTGCACCAC CAGTTGGAAA ATCGTTCTCT TCATGAAATG GAGCGACAAC 
CAGGAGATTG TCTTGAAAGG AGGTGACGTG GTGAGGCTGT TTCATGCAGA GCAGGAGAAG 
TTTCTGACAT GTGATGAGCA TCGGAAAAAG CAGTATGTGT TTCTGCGTAC CACCGGACGC 
CAGTCTGCAA CCTCCGCCAC TAGTAGCAAA GCACTCTGGG AGGTAGAGGT GGTTCAGCAT 
GATCCCTGCC GAGGCGGAGC CGGCTACTGG AACAGCCTCT TTAGGTTTAA ACACTTAGCC 
ACCGGCCATT ACCTTGCTGC AGAGATGGAT TCGGAGCATG AAGTGATTCG GGCTCGTGCA 
CGTAACCCTC AGGATAAGGT GATGTACACA CTGGTGTCTG TTCCTGATGG AAATGACATA 
TCCTCCATCT TTGAGCTTGA CCCTACAACT TTGAGGGGCG GAGACTCACT TGTGCCAAGA 
AACTCGTATG TGAGGCTCAG GCATCTCTGC ACCAACACGT GGGTCCACAG CACCAACCAG 
CCAATAGATA AAGAGGAAGA GAAACCGGTC ATGCTCAGAA TAGGAACGTC TCCTCTGAAG 
GAAGATAAAG AGGCGTTTGC TATAGTACCA GTCTCTCCAG CTGAGGTTCG AGATCTGGAT 
TTTGCTAATG ATGCTAGTAA AGTGTTGGCC TCCATTGCTG CCAAACTGGA GAAGGGCACC 
ATCACCCAGA ATGAGAGGAG GTCAGTGACG AAGCTGTTGG AGGATCTGGT GTATTTTGTG 
GTGGACATAC CCAGCAGTGC TCAAGATGTG CTGGAGATCA CAGTCAACAA ACCCAACAGA 
GAGCGACAGA AACTCATGAG AGAGCAGAAC ATTCTTAAAC AGATCTTCAA GCTGCTGCAG 
GCGCCATTTA CAGACAGCGG GGACGGCCCT ATGTTGCGAT TAGAAGAGCT CGCAGACCAG 
CGTCATGCAC CCTTTCGACA CATTTGCAGA CTCTGTTACA GAGTCCTACG TTACTCACAA 
CAGGACTATC GCAAAAATCA GGAGTACATA GCAAAGCAGT TCCGCTTCAT GCAGAAGCAG 
ATAGGATATG ATGTGCTGGC TGAAGACACA ATTACAGCCC TTCTGCACAA CAACAGGAAG 
CTCCTAGAGA AACACATCAC AGCAGCTGAA ATAGATACCT TTGTCAGTCT GGTCCGCAAG 
AACAGAGAGC CCAGATTTCT GGATTACTTG TCAGATCTGT GTGTGTCGAT GAATAAATCC 
ATCCCGGTCA CTCAAGAGCT CATCTGTAAT GCTGTGCTGG ACCCAGCCAA TGCAGACATT 
CTCATCGAAA CCAAGCTTGT GTTGTCACGA TTTGAGGTGG CAGGAACGGT GTTGGGTGAA 
GGTGCGGAGG AGGAAGAGGA GGATGAAGAG GAGGTCTGGC TGTTCTGGAA GGACAGTGGA 
GGAGAGGTGA AGAGTAAAAG CATACGAGAG CTTGCACAGG ACGCAAAAGA TGGACATACA 
GAGGACCAGG AAGTCATCAA TTATTACAGA TATCAGCTGA ATTTGTTTGC TCGGATGTGT 
CTGGATCGAC AGTATCTTGC GATTAACAAG ATATCAGCAC AGCTGGATGT GGATCTGATT 
CTGCGCTGCA TGTCAGATGA AGATTTGCCC TTTGACCTCA GAGCATCGTT CTGCCGCATG 
ATGCTGCACA TGCATGTAGA CCGTGACCCT CAGGAGCAGG TCACCCCGGT CAAATATGCT 
CGACTGTGGT CTGAAATCCC ATCACAAATT TCCATTGATG ATTATGATAA TGATGGGACG 
TCACGTGATG AAGTGAAAGA GCGCTTCTCT CAGACGATGG AGTTTGTGGA GAATTACCTG 
AGGGATGTTG TCTGCCAGAG TTTCCCGTTC TCTGATAAGG AGAAAAACAA ACTCACTTTT 
GAGGTGGTGA ACCTGGCAAG AAACCTGATC TATTTTGGTT TCTATAACTT CAGTGATCTG 
CTAAGGTTAA CAAAAATCCT ATTGGCAATC CTGGACTGTG TCCACATAAG CACTCCCTTC 
CACATCAAGC TGGATAGAGA ACCTGGTAAA GGAAGTAACG TGATGCGCTC CATCCATGGG 
GTGGGAGAAC TGATGACACA GGTAGTGTTG AGGGGTGGGG GATTTCTGCC AGCCTCCAGC 
CACAATCCTC CTGACAGAGA CGAAGTGAAA GCACAAAGTG AACCTCAGAA ACAAGACATA 
CTGGTGATGG ACACTAAACT CAAGATCATC GAGATCCTGC AGTTTATTCT GAATGTGCGG 
TTGGACTACA GGATCTCCTG CCTCCTCTGT ATCTTTAAGA CAGAGTTTGA TGAAAGTAAC 
TCTCAAAGTG AACCATCTTT AAGCCCAGAA TCTCCTGCCA GCGTTCAAGG TGCGCTAGAC 
TTTGAGCACA TTGAAGAGCA GGCGGAGGGT ATTTTTGGAG GAAGTGAAGA GAACACCCCG 
CTGGATCTTG ATGATCATGG TGGACGGACA TTTCTGAGGG TCCTGCTTCA CCTGACCATG 
CATGACTATC CTCCGCTGGT ATCTCGAGCT CTTCACCTGC TCTTCAGACA CTTTAGCCAA 
CGCCAGGAGG TCCTACAGGC CTTCAAACAG GTTCAGCTCC TGGTGACCAG TCAGGATGTG 
GAAAACTATA AACAGATCAA GTCAGATTTG GACCAGCTGA GGTCAATAGT AGAGAAATCA 
GAGTTGTGGG TGTATAAGAG ACAAGGCTCA GACTCAGGAC TGGATGCTGG AGAAGTCACC 
CCTGAAGCCC ACCACAAGGG TGTCATCAAC TCGAGTAGAT CAAACAAACC CAAAGTGGAA 
AGTACCAGCA GCTCGAACTA CAGAGTCGTG AAAGAGATCC TGCTGAGGCT GAGCAAACTG 
TGTGTTTTGG AGGGCATCTC TGGCAAGAAG AATAAGAAAC AGCAGCAGCG GCTCCTCAGG 
AACATGGGAG CTCACAGTGT GGTCCTCGAA CTGTTGCAAA TCCCTTATGA GAAGGGGGAG 
GATGTCCAGA TGCAGGAGAT CATGACCTTA GCACATCAGT TCCTTCAAAA CTTCTGTGCT 
GGAAATCAGC AGAACCAGGC ACTTCTACAC AAACACATCA ACCTGTTCCT CAACCCTGGG 
ATCCTGGAGG CGGTGACCAT GCAGCATATC TTCATGAATA ATTTCCAGTT GTGTAGTGAG 
ATTAATGAGC GGGTCGTGCA GCATTTTGTG CACTGCATCG AGACACACGG CCGCAGCGTG 
CACTACCTCA AGTTCCTGCA GACCATTGTC AAGGCAGAGA ACAAGTTCAT CAAGAAATGC 
CAAGACATCG TCATGGCTGA GCTGGTGACT GCAGGTGAGG ATGTGCTGGT GTTCTATAAT 
GACCGGGCGT CTTTTCAGTC ATTGGTTCAG ATGATGCGTC TTGAGCGGGA ACGACTTGAT 
GAGAGCAGTG CACTCAGGTA TCACATTCAC CTGGTGGAGT TGCTGGCTGT GTGCACTGAA 
GGCAAGAACG TCTACACTGA GATCAAATGC AACTCGCTGC TCCCTCTCGA TGATATTGTA 
CGTGTGGTCA CACATGAGGA CTGCATCCCT GAGGTGAAGA TGGCCTACGT GAACTTCCTG 
AATCACTGTT ATGTTGATAC AGAGGTTGAG ATGAAGGAGA TCTACACCAG CAATCACATG 
TGGAAACTTT TTGATGATTT TCTGGTGGAC GTCTGCAGAG TGTGCAATAA CACAAGTGAC 
CGTAAACACG CTGACACAGT TCTGGAGAGA TACGTTACAG AGACCATAAT GAGCATCGTC 
ACCACCTTTT TCAGCTCACC ATTCTCAGAC CAGAGCACAA GTTTGCAGAC CCGGCAGCCG 
GTGTTTGTGC AGCTGCTGCA GGGAGTGTTT CGTGTATATC ACTGCAACTG GCTTCTCCCA 
GGTCAGAAGG GGAATGTGGA AGCCTGCATC AAAGTGCTGA GTGACGTGGC CAAAGGCAGA 
GCCATAGCCA TTCCTGTGGA TCTGGACTGT CAGGTGAATA ATCTGTTCAT GAAGTCCAAC 
AACATTGTGC AGAAAACAGC CCTCAGCTGG AGACTCTCTG TCCGTAACGC GACTCGGAGA 
GACTCTGTTC TGACCACCTC TCGAGACTAT CGCAACATCA TTGAAAGACT ACAGGATATA 
GTGTCTGCAT TGGAGGAGCG GTTGAGGCCT CTGGTTCAGG CTGAGTTGTC AGTGTTGGTG 
GATGTTCTCC ATCGCCCAGA GCTGCTGTTT CCAGAGCACA CTGAAGCCCA CCGCAAGTGT 
GAGAGTGGAG GCTTCATCTG CAAGTTGATA AAACACACTA AGCAGCTTCT AGAAGAAAAC 
GAAGAGAGGC TTTGCATCAA AGTCCTTCAG ACACTCCGAG AGATGATGAC CAAAGACAGA 
GGATATGGAG AGAAGCTTCT GGGATATGAT GATGAGATGG ATGTCACGGA GGTGGTGGAT 
ATCAGTCTGC CACCCAAACA GCAGGAGGAT CGGCAGAGGG GGGAGGCCCT TCGGCAGCTG 
CTCGTCAATC GTTACTATGG CTTCAGGAGT GGTGGGCGGA GAGAAAGTCT GACCTCGTTT 
GGTAACTCCA CCCTAACTCC TGTCGGGCCA ATCAAGAGCC AGCCAGGGGG GTTGAGCAGG 
GCTGAGATGT CATTAATGGA GGTGCAGTGT CATCTAGACC GTGAAGGGGC ATCGGATCTT 
GTCATTGATC TCATCATGAA CACTACCAGC GACCGCGTCT TCCATGAAAG CATTCTGTTG 
GCCATCGCAC TGTTGGAGGG CGGAAACACC ATCATACAGC ATTCCTTCTT CAAACGTTTA 
ACAGAAGACA AAAATTCAGA GAAGTTTTTC AGGGTGTTTT ATGACAGAAT GAAAGTGGCT 
CAGGTGGAGA TCAAAGCCAC GGTTACTGTC AACACTAGTG ACCTCGGCAA CAAGCGGCGT 
GACGACAACA CTCCAGACAA AGATACTCCA CAACGCCGCA GAGGTAAAGA CTCCGGTGTG 
GTGGTGACAG ATGATGCTCG TGAGCAGCTG TTGGAGGCTT CAGCTGCGAC TAAGAAGGCC 
TTTGGTTCAT ATCGTCGAGA TGCTGATCCC GAGGAGACGT TTGGCACGGC TGATGGAGAT 
AAAGGAGGTG GAGATAAAGG CACAGAGCAG GGCGAGATGA GCCCTGTCAT CCTCATAATG 
CAGCCAATCC TGCGCTTCCT GCAGCTGCTG TGCGAAAACC ACAATCGAGA CCTGCAGAAT 
TTTCTGCGCT GTCAGAACAA TAAAAACAAC TATAATCTGG TGTGTGAGAC GCTGCAGTTC 
CTGGACTGTA TCTGTGGCAG CACAACAGGA GGCCTGGGGC TGTTAGGACT CTACATCAAC 
CAGCATAACG TAGCGCTCAT TAATCAGACT GTAGAGAGCC TCACTGAATA CTGTCAAGGT 
CCCTGCCATG ACAATCAGAA TTGCATTGCC ACCCATGAGT CTAATGGCAT CGACATCATT 
ATTGCACTGA TTCTGAATGA TATTAATCCA CTGGGCAGGA AGAGAATGGA TCTGGTTCTA 
GAACTAAAGA ATAATGCATC TAAGCTGCTG CTGGCCATCA TGGAGAGCCG TCATGACAGT 
GAGAATGCAG AAAGAATTCT GTACAACATG AGACCAAAAG AACTAGTGGA GGTGATGAAA 
ATGGCCTACC AGCAGGGGGA GGCAGAGTTT GAGGATGAGG AACAGGAGAA TGGAGAGGAT 
CATGCAGCAT CGCCTCGCAA TGTTGGACAC AACATCTATA TTCTTGCACA TCAGCTGTCG 
CGTCACAATA AGGAATTGCA AATACTACTG AAACCAGGTG GAGAGGACCA GGCCCTGGAG 
TATTACACAA AACACACCTC ACAGATAGAG ATAGTTCGAC AGGATCGCAC TATGGAACAG 
ATTGTGTTTC CTGTACCAAA TATCTGCTCT TTTCTGACAA ATGAGTCCAA GCTGCGTGTG 
TACTATGGTA CAGAGAGAGA CGAGCAAGGC AGTAAAATAA ATGACTTCTT CTTGCATGCA 
GACGACCTCT TTAATGAAAT GAGGTGGCAG AAAAAACTAA GAGCTCAGCC AGTGCTGTAC 
TGGTGCAGCA GGAACATGTC GTTTTGGAGT AACGTTTCCT TTAATCTGGC GGTGCTCATT 
AATTTGCTGG TGGCATTTTT CTACCCTCTG GATGGAGTGA GCGAAAGCCA GCTGGAACCT 
TCTCTGTCTC TGTTGCTGTG GGTTTGTTTG TTGGGCTCAC TGGGCTTCGT GTTGATGTCC 
CCGAGGCCAA ATGCAGTGCG GGTGCTGGTC ATCTCCACTG TCCTGCAGTT GGGCTTCTCT 
GTAGGGCTGC AGCACATGCT CACACTCCTG GGAGCTTTCA ATGTTTGTAA TAAGATCGTG 
TTCATGCTGA GCTTTGTGGG TAACCGTGGG TCATTCACCC GGGGTTATCG GGTGATGGTC 
ATGGATAGAG AGTTCCTGTT TCATCTGCTG TACCTTCTCA TCTGCATACT GGGCCTTTTT 
GGACACGTCT TCTTTTACAG TCTTCTGTTG TTTGACCTGG TGAACCGGGA AGAGACTCTT 
CTGAACGTCA TCAAGAGTGT GACTCGAAAC GGCCGCTCCA TCGTCTTGAC CGCAGTGCTG 
GGCCTCATCC TGGTCTACCT CTTTTCTATT GTTGGATATA TGTTTTTCAA AGATGACTTC 
ATCCTGGAGG TGAACCGCAT CTCCAACGGC ACTCTGGAGG AGGGGGTGAC TTTACCTGCT 
GGTTTTTGTG ATGCGAGAAA CGGGTCGTGT CTTTCTGATG ATGTTGACGA GGATGACGTG 
GAGCGCGCGT GTGATTCTCT ATGGATGTGT ATGATCACTG TACTCAGTCA TGGCCTCAGG 
AGCGGTGGAG GAGTTGGAGA TGTCCTGCGC AAACCGTCTA AAGAGGAGCG TCTGTTCGCC 
GCACGGGTGG TGTACGATCT CCTGTTCTTC TTCCTGGTGA TCATCATTGT GTTGAATCTG 
ATCTTCGGTG TGATCATTGA CACATTTGCA GATCTGAGAA GCGAGAAGCA GAGAAAAGAG 
GAGATCTTGA AGACCACATG CTTCATCTGT GGTCTGGAGA GAGATAAGTT TGATAATAAA 
ACGGTGACGT TTGAAGAGCA CATCAAGGAG GAGCACAACC TGTGGCATTA CCTCTTCTTC 
ATAGTGCTGG TGCGGGTCAA AGACTCCACC GAATACACGG GACCCGAGAG CTACGTCGCT 
CAGATGATCA AGGAGCACAA TCTGGACTGG TTTCCTCGTA TGAGGGCGAT GTCTCTGGTC 
AGCAGTGATG GAGAAGGTGA ACAGAATGAA CTGCGCAGCT TACAGGAGAA ACTCGAGTCC 
ACAATGAGGC TCGTCTCTAA TCTTACCAAT CAGCTCACAG AGCTCAAAGA GCAGATGACT 
GAGCAAAGGA AACACAAGCA GAGGCTCGGT CTGCTGGGAA ACCCCGCCCA CCTCAACATC 
AACCCACAGC AGCCAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itpr1a ( XM_021474617.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330292.1 Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X13, mRNA.

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CloneID	ODa146876
Clone ID Related Accession (Same CDS sequence)	XM_021474617.1
Accession Version	XM_021474617.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8085bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395382.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACAT TACAAGGTGC TGGAGTTTTT GCCATGGTGG ACAAATTGTC TAGTTTCCTG 
CACATTGGGG ACGTTTGTTC CCTTTATGCT GAAGGCTCTA CTAGTGGCTT CATCAGCACT 
CTGGGGTTGG TTGATGACCG ATGTGTTGTT CAACCAGATG CCGGAGACCT GAATAACCCA 
CCTAAAAAGT TTCGGGACTG TCTGTTCAGA CTGTGTCCCA TGAACAGATA TTCGGCACAG 
AAACAGTTCT GGAAAGCTGC CAAACCTGGA GGAACTAGCA CCACAGATAC TGTCCTGCTT 
AACAAACTCC ATCATGCTGC TGATTTAGAG AAGAAACAAA ATGATTCTGA GAACAAAAAG 
CTGCTTGGAA CCGTCATTCA GTATGGCAAT GTCATCCAGC TTCTCCATCT GAAGAGTAAT 
AAATATCTGA CGGTGAATAA GCGTTTGCCG GCTCTTCTTG AGAAGAATGC CATGCGTGTG 
ACTCTGGACG CTGCAGGGAA TGAAGGCTCG TGGTTTTACA TCCAGCCCTT CTATAAGCTG 
CGCTCTATAG GGGATAATGT GGTCATTGGA GATAAGGTCG TGTTAAACCC TGTCAATGCC 
GGACAGCCGC TGCACGCCAG CAGCCATCAG CTTGTGGATA ATCCAGGCTG CAATGAAGTG 
AATTCAGTGA ACTGCACCAC CAGTTGGAAA ATCGTTCTCT TCATGAAATG GAGCGACAAC 
CAGGAGATTG TCTTGAAAGG AGGTGACGTG GTGAGGCTGT TTCATGCAGA GCAGGAGAAG 
TTTCTGACAT GTGATGAGCA TCGGAAAAAG CAGTATGTGT TTCTGCGTAC CACCGGACGC 
CAGTCTGCAA CCTCCGCCAC TAGTAGCAAA GCACTCTGGG AGGTAGAGGT GGTTCAGCAT 
GATCCCTGCC GAGGCGGAGC CGGCTACTGG AACAGCCTCT TTAGGTTTAA ACACTTAGCC 
ACCGGCCATT ACCTTGCTGC AGAGGTAAAT CCAGACTATG AAGAAGAATG CCTGGAGTCC 
CGCTCCTCTA TGGATTCGGA GCATGAAGTG ATTCGGGCTC GTGCACGTAA CCCTCAGGAT 
AAGGTGATGT ACACACTGGT GTCTGTTCCT GATGGAAATG ACATATCCTC CATCTTTGAG 
CTTGACCCTA CAACTTTGAG GGGCGGAGAC TCACTTGTGC CAAGAAACTC GTATGTGAGG 
CTCAGGCATC TCTGCACCAA CACGTGGGTC CACAGCACCA ACCAGCCAAT AGATAAAGAG 
GAAGAGAAAC CGGTCATGCT CAGAATAGGA ACGTCTCCTC TGAAGGAAGA TAAAGAGGCG 
TTTGCTATAG TACCAGTCTC TCCAGCTGAG GTTCGAGATC TGGATTTTGC TAATGATGCT 
AGTAAAGTGT TGGCCTCCAT TGCTGCCAAA CTGGAGAAGG GCACCATCAC CCAGAATGAG 
AGGAGGTCAG TGACGAAGCT GTTGGAGGAT CTGGTGTATT TTGTGGTGGA CATACCCAGC 
AGTGCTCAAG ATGTGCTGGA GATCACAGTC AACAAACCCA ACAGAGAGCG ACAGAAACTC 
ATGAGAGAGC AGAACATTCT TAAACAGATC TTCAAGCTGC TGCAGGCGCC ATTTACAGAC 
AGCGGGGACG GCCCTATGTT GCGATTAGAA GAGCTCGCAG ACCAGCGTCA TGCACCCTTT 
CGACACATTT GCAGACTCTG TTACAGAGTC CTACGTTACT CACAACAGGA CTATCGCAAA 
AATCAGGAGT ACATAGCAAA GCAGTTCCGC TTCATGCAGA AGCAGATAGG ATATGATGTG 
CTGGCTGAAG ACACAATTAC AGCCCTTCTG CACAACAACA GGAAGCTCCT AGAGAAACAC 
ATCACAGCAG CTGAAATAGA TACCTTTGTC AGTCTGGTCC GCAAGAACAG AGAGCCCAGA 
TTTCTGGATT ACTTGTCAGA TCTGTGTGTG TCGATGAATA AATCCATCCC GGTCACTCAA 
GAGCTCATCT GTAATGCTGT GCTGGACCCA GCCAATGCAG ACATTCTCAT CGAAACCAAG 
CTTGTGTTGT CACGATTTGA GGTGGCAGGA ACGGTGTTGG GTGAAGGTGC GGAGGAGGAA 
GAGGAGGATG AAGAGGAGGT CTGGCTGTTC TGGAAGGACA GTGGAGGAGA GGTGAAGAGT 
AAAAGCATAC GAGAGCTTGC ACAGGACGCA AAAGATGGAC ATACAGAGGA CCAGGAAGTC 
ATCAATTATT ACAGATATCA GCTGAATTTG TTTGCTCGGA TGTGTCTGGA TCGACAGTAT 
CTTGCGATTA ACAAGATATC AGCACAGCTG GATGTGGATC TGATTCTGCG CTGCATGTCA 
GATGAAGATT TGCCCTTTGA CCTCAGAGCA TCGTTCTGCC GCATGATGCT GCACATGCAT 
GTAGACCGTG ACCCTCAGGA GCAGGTCACC CCGGTCAAAT ATGCTCGACT GTGGTCTGAA 
ATCCCATCAC AAATTTCCAT TGATGATTAT GATAATGATG GGACGTCACG TGATGAAGTG 
AAAGAGCGCT TCTCTCAGAC GATGGAGTTT GTGGAGAATT ACCTGAGGGA TGTTGTCTGC 
CAGAGTTTCC CGTTCTCTGA TAAGGAGAAA AACAAACTCA CTTTTGAGGT GGTGAACCTG 
GCAAGAAACC TGATCTATTT TGGTTTCTAT AACTTCAGTG ATCTGCTAAG GTTAACAAAA 
ATCCTATTGG CAATCCTGGA CTGTGTCCAC ATAAGCACTC CCTTCCACAT CAAGCTGGAT 
AGAGAACCTG GTAAAGGAAG TAACGTGATG CGCTCCATCC ATGGGGTGGG AGAACTGATG 
ACACAGGTAG TGTTGAGGGG TGGGGGATTT CTGCCAGCCT CCAGCCACAA TCCTCCTGAC 
AGAGACGAAG TGAAAGCACA AAGTGAACCT CAGAAACAAG ACATACTGGT GATGGACACT 
AAACTCAAGA TCATCGAGAT CCTGCAGTTT ATTCTGAATG TGCGGTTGGA CTACAGGATC 
TCCTGCCTCC TCTGTATCTT TAAGACAGAG TTTGATGAAA GTAACTCTCA AAGTGAACCA 
TCTTTAAGCC CAGAATCTCC TGCCAGCGTT CAAGGTGCGC TAGACTTTGA GCACATTGAA 
GAGCAGGCGG AGGGTATTTT TGGAGGAAGT AGTGAAGAGA ACACCCCGCT GGATCTTGAT 
GATCATGGTG GACGGACATT TCTGAGGGTC CTGCTTCACC TGACCATGCA TGACTATCCT 
CCGCTGGTAT CTCGAGCTCT TCACCTGCTC TTCAGACACT TTAGCCAACG CCAGGAGGTC 
CTACAGGCCT TCAAACAGGT TCAGCTCCTG GTGACCAGTC AGGATGTGGA AAACTATAAA 
CAGATCAAGT CAGATTTGGA CCAGCTGAGG TCAATAGTAG AGAAATCAGA GTTGTGGGTG 
TATAAGAGAC AAGGCTCAGA CTCAGGACTG GATGCTGGAG AAGTCACCCC TGAAGCCCAC 
CACAAGGGTG TCATCAACTC GAGTAGATCA AACAAACCCA AAGTGGAAAG TACCAGCAGC 
TCGAACTACA GAGTCGTGAA AGAGATCCTG CTGAGGCTGA GCAAACTGTG TGTTTTGGAG 
GGCATCTCTG GCAAGAAGAA TAAGAAACAG CAGCAGCGGC TCCTCAGGAA CATGGGAGCT 
CACAGTGTGG TCCTCGAACT GTTGCAAATC CCTTATGAGA AGGGGGAGGA TGTCCAGATG 
CAGGAGATCA TGACCTTAGC ACATCAGTTC CTTCAAAACT TCTGTGCTGG AAATCAGCAG 
AACCAGGCAC TTCTACACAA ACACATCAAC CTGTTCCTCA ACCCTGGGAT CCTGGAGGCG 
GTGACCATGC AGCATATCTT CATGAATAAT TTCCAGTTGT GTAGTGAGAT TAATGAGCGG 
GTCGTGCAGC ATTTTGTGCA CTGCATCGAG ACACACGGCC GCAGCGTGCA CTACCTCAAG 
TTCCTGCAGA CCATTGTCAA GGCAGAGAAC AAGTTCATCA AGAAATGCCA AGACATCGTC 
ATGGCTGAGC TGGTGACTGC AGGTGAGGAT GTGCTGGTGT TCTATAATGA CCGGGCGTCT 
TTTCAGTCAT TGGTTCAGAT GATGCGTCTT GAGCGGGAAC GACTTGATGA GAGCAGTGCA 
CTCAGGTATC ACATTCACCT GGTGGAGTTG CTGGCTGTGT GCACTGAAGG CAAGAACGTC 
TACACTGAGA TCAAATGCAA CTCGCTGCTC CCTCTCGATG ATATTGTACG TGTGGTCACA 
CATGAGGACT GCATCCCTGA GGTGAAGATG GCCTACGTGA ACTTCCTGAA TCACTGTTAT 
GTTGATACAG AGGTTGAGAT GAAGGAGATC TACACCAGCA ATCACATGTG GAAACTTTTT 
GATGATTTTC TGGTGGACGT CTGCAGAGTG TGCAATAACA CAAGTGACCG TAAACACGCT 
GACACAGTTC TGGAGAGATA CGTTACAGAG ACCATAATGA GCATCGTCAC CACCTTTTTC 
AGCTCACCAT TCTCAGACCA GAGCACAAGT TTGCAGACCC GGCAGCCGGT GTTTGTGCAG 
CTGCTGCAGG GAGTGTTTCG TGTATATCAC TGCAACTGGC TTCTCCCAGG TCAGAAGGGG 
AATGTGGAAG CCTGCATCAA AGTGCTGAGT GACGTGGCCA AAGGCAGAGC CATAGCCATT 
CCTGTGGATC TGGACTGTCA GGTGAATAAT CTGTTCATGA AGTCCAACAA CATTGTGCAG 
AAAACAGCCC TCAGCTGGAG ACTCTCTGTC CGTAACGCGA CTCGGAGAGA CTCTGTTCTG 
ACCACCTCTC GAGACTATCG CAACATCATT GAAAGACTAC AGGATATAGT GTCTGCATTG 
GAGGAGCGGT TGAGGCCTCT GGTTCAGGCT GAGTTGTCAG TGTTGGTGGA TGTTCTCCAT 
CGCCCAGAGC TGCTGTTTCC AGAGCACACT GAAGCCCACC GCAAGTGTGA GAGTGGAGGC 
TTCATCTGCA AGTTGATAAA ACACACTAAG CAGCTTCTAG AAGAAAACGA AGAGAGGCTT 
TGCATCAAAG TCCTTCAGAC ACTCCGAGAG ATGATGACCA AAGACAGAGG ATATGGAGAG 
AAGGGGGAGG CCCTTCGGCA GCTGCTCGTC AATCGTTACT ATGGCTTCAG GAGTGGTGGG 
CGGAGAGAAA GTCTGACCTC GTTTGGTAAC TCCACCCTAA CTCCTGTCGG GCCAATCAAG 
AGCCAGCCAG GGGGGTTGAG CAGGGCTGAG ATGTCATTAA TGGAGGTGCA GTGTCATCTA 
GACCGTGAAG GGGCATCGGA TCTTGTCATT GATCTCATCA TGAACACTAC CAGCGACCGC 
GTCTTCCATG AAAGCATTCT GTTGGCCATC GCACTGTTGG AGGGCGGAAA CACCATCATA 
CAGCATTCCT TCTTCAAACG TTTAACAGAA GACAAAAATT CAGAGAAGTT TTTCAGGGTG 
TTTTATGACA GAATGAAAGT GGCTCAGGTG GAGATCAAAG CCACGGTTAC TGTCAACACT 
AGTGACCTCG GCAACAAGCG GCGTGACGAC AACACTCCAG ACAAAGATAC TCCACAACGC 
CGCAGAGGTA AAGACTCCGG TGTGGTGGTG ACAGATGATG CTCGTGAGCA GCTGTTGGAG 
GCTTCAGCTG CGACTAAGAA GGCCTTTGGT TCATATCGTC GAGATGCTGA TCCCGAGGAG 
ACGTTTGGCA CGGCTGATGG AGATAAAGGA GGTGGAGATA AAGGCACAGA GCAGGGCGAG 
ATGAGCCCTG TCATCCTCAT AATGCAGCCA ATCCTGCGCT TCCTGCAGCT GCTGTGCGAA 
AACCACAATC GAGACCTGCA GAATTTTCTG CGCTGTCAGA ACAATAAAAA CAACTATAAT 
CTGGTGTGTG AGACGCTGCA GTTCCTGGAC TGTATCTGTG GCAGCACAAC AGGAGGCCTG 
GGGCTGTTAG GACTCTACAT CAACCAGCAT AACGTAGCGC TCATTAATCA GACTGTAGAG 
AGCCTCACTG AATACTGTCA AGGTCCCTGC CATGACAATC AGAATTGCAT TGCCACCCAT 
GAGTCTAATG GCATCGACAT CATTATTGCA CTGATTCTGA ATGATATTAA TCCACTGGGC 
AGGAAGAGAA TGGATCTGGT TCTAGAACTA AAGAATAATG CATCTAAGCT GCTGCTGGCC 
ATCATGGAGA GCCGTCATGA CAGTGAGAAT GCAGAAAGAA TTCTGTACAA CATGAGACCA 
AAAGAACTAG TGGAGGTGAT GAAAATGGCC TACCAGCAGG GGGAGGCAGA GTTTGAGGAT 
GAGGAACAGG AGAATGGAGA GGATCATGCA GCATCGCCTC GCAATGTTGG ACACAACATC 
TATATTCTTG CACATCAGCT GTCGCGTCAC AATAAGGAAT TGCAAATACT ACTGAAACCA 
GGTGGAGAGG ACCAGGCCCT GGAGTATTAC ACAAAACACA CCTCACAGAT AGAGATAGTT 
CGACAGGATC GCACTATGGA ACAGATTGTG TTTCCTGTAC CAAATATCTG CTCTTTTCTG 
ACAAATGAGT CCAAGCTGCG TGTGTACTAT GGTACAGAGA GAGACGAGCA AGGCAGTAAA 
ATAAATGACT TCTTCTTGCA TGCAGACGAC CTCTTTAATG AAATGAGGTG GCAGAAAAAA 
CTAAGAGCTC AGCCAGTGCT GTACTGGTGC AGCAGGAACA TGTCGTTTTG GAGTAACGTT 
TCCTTTAATC TGGCGGTGCT CATTAATTTG CTGGTGGCAT TTTTCTACCC TCTGGATGGA 
GTGAGCGAAA GCCAGCTGGA ACCTTCTCTG TCTCTGTTGC TGTGGGTTTG TTTGTTGGGC 
TCACTGGGCT TCGTGTTGAT GTCCCCGAGG CCAAATGCAG TGCGGGTGCT GGTCATCTCC 
ACTGTCCTGC AGTTGGGCTT CTCTGTAGGG CTGCAGCACA TGCTCACACT CCTGGGAGCT 
TTCAATGTTT GTAATAAGAT CGTGTTCATG CTGAGCTTTG TGGGTAACCG TGGGTCATTC 
ACCCGGGGTT ATCGGGTGAT GGTCATGGAT AGAGAGTTCC TGTTTCATCT GCTGTACCTT 
CTCATCTGCA TACTGGGCCT TTTTGGACAC GTCTTCTTTT ACAGTCTTCT GTTGTTTGAC 
CTGGTGAACC GGGAAGAGAC TCTTCTGAAC GTCATCAAGA GTGTGACTCG AAACGGCCGC 
TCCATCGTCT TGACCGCAGT GCTGGGCCTC ATCCTGGTCT ACCTCTTTTC TATTGTTGGA 
TATATGTTTT TCAAAGATGA CTTCATCCTG GAGGTGAACC GCATCTCCAA CGGCACTCTG 
GAGGAGGGGG TGACTTTACC TGCTGGTTTT TGTGATGCGA GAAACGGGTC GTGTCTTTCT 
GATGATGTTG ACGAGGATGA CGTGGAGCGC GCGTGTGATT CTCTATGGAT GTGTATGATC 
ACTGTACTCA GTCATGGCCT CAGGAGCGGT GGAGGAGTTG GAGATGTCCT GCGCAAACCG 
TCTAAAGAGG AGCGTCTGTT CGCCGCACGG GTGGTGTACG ATCTCCTGTT CTTCTTCCTG 
GTGATCATCA TTGTGTTGAA TCTGATCTTC GGTGTGATCA TTGACACATT TGCAGATCTG 
AGAAGCGAGA AGCAGAGAAA AGAGGAGATC TTGAAGACCA CATGCTTCAT CTGTGGTCTG 
GAGAGAGATA AGTTTGATAA TAAAACGGTG ACGTTTGAAG AGCACATCAA GGAGGAGCAC 
AACCTGTGGC ATTACCTCTT CTTCATAGTG CTGGTGCGGG TCAAAGACTC CACCGAATAC 
ACGGGACCCG AGAGCTACGT CGCTCAGATG ATCAAGGAGC ACAATCTGGA CTGGTTTCCT 
CGTATGAGGG CGATGTCTCT GGTCAGCAGT GATGGAGAAG GTGAACAGAA TGAACTGCGC 
AGCTTACAGG AGAAACTCGA GTCCACAATG AGGCTCGTCT CTAATCTTAC CAATCAGCTC 
ACAGAGCTCA AAGAGCAGAT GACTGAGCAA AGGAAACACA AGCAGAGGCT CGGTCTGCTG 
GGAAACCCCG CCCACCTCAA CATCAACCCA CAGCAGCCAG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330292.1
CDS	265..8349
Translation	MMTLQGAGVFAMVDKLSSFLHIGDVCSLYAEGSTSGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGTSTTDTVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDAAGNEGSWFYIQPFYKLRSIGDNVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCTTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDEHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEVNPDYEEECLESRSSMDSEHEVIRARARNPQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSPLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAAKLEKGTITQNERRSVTKLLEDLVYFVVDIPSSAQDVLEITVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRYSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDPANADILIETKLVLSRFEVAGTVLGEGAEEEEEDEEEVWLFWKDSGGEVKSKSIRELAQDAKDGHTEDQEVINYYRYQLNLFARMCLDRQYLAINKISAQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSQISIDDYDNDGTSRDEVKERFSQTMEFVENYLRDVVCQSFPFSDKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHISTPFHIKLDREPGKGSNVMRSIHGVGELMTQVVLRGGGFLPASSHNPPDRDEVKAQSEPQKQDILVMDTKLKIIEILQFILNVRLDYRISCLLCIFKTEFDESNSQSEPSLSPESPASVQGALDFEHIEEQAEGIFGGSSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALHLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSDSGLDAGEVTPEAHHKGVINSSRSNKPKVESTSSSNYRVVKEILLRLSKLCVLEGISGKKNKKQQQRLLRNMGAHSVVLELLQIPYEKGEDVQMQEIMTLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAVTMQHIFMNNFQLCSEINERVVQHFVHCIETHGRSVHYLKFLQTIVKAENKFIKKCQDIVMAELVTAGEDVLVFYNDRASFQSLVQMMRLERERLDESSALRYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKMAYVNFLNHCYVDTEVEMKEIYTSNHMWKLFDDFLVDVCRVCNNTSDRKHADTVLERYVTETIMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQGVFRVYHCNWLLPGQKGNVEACIKVLSDVAKGRAIAIPVDLDCQVNNLFMKSNNIVQKTALSWRLSVRNATRRDSVLTTSRDYRNIIERLQDIVSALEERLRPLVQAELSVLVDVLHRPELLFPEHTEAHRKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKGEALRQLLVNRYYGFRSGGRRESLTSFGNSTLTPVGPIKSQPGGLSRAEMSLMEVQCHLDREGASDLVIDLIMNTTSDRVFHESILLAIALLEGGNTIIQHSFFKRLTEDKNSEKFFRVFYDRMKVAQVEIKATVTVNTSDLGNKRRDDNTPDKDTPQRRRGKDSGVVVTDDAREQLLEASAATKKAFGSYRRDADPEETFGTADGDKGGGDKGTEQGEMSPVILIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINQHNVALINQTVESLTEYCQGPCHDNQNCIATHESNGIDIIIALILNDINPLGRKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVMKMAYQQGEAEFEDEEQENGEDHAASPRNVGHNIYILAHQLSRHNKELQILLKPGGEDQALEYYTKHTSQIEIVRQDRTMEQIVFPVPNICSFLTNESKLRVYYGTERDEQGSKINDFFLHADDLFNEMRWQKKLRAQPVLYWCSRNMSFWSNVSFNLAVLINLLVAFFYPLDGVSESQLEPSLSLLLWVCLLGSLGFVLMSPRPNAVRVLVISTVLQLGFSVGLQHMLTLLGAFNVCNKIVFMLSFVGNRGSFTRGYRVMVMDREFLFHLLYLLICILGLFGHVFFYSLLLFDLVNREETLLNVIKSVTRNGRSIVLTAVLGLILVYLFSIVGYMFFKDDFILEVNRISNGTLEEGVTLPAGFCDARNGSCLSDDVDEDDVERACDSLWMCMITVLSHGLRSGGGVGDVLRKPSKEERLFAARVVYDLLFFFLVIIIVLNLIFGVIIDTFADLRSEKQRKEEILKTTCFICGLERDKFDNKTVTFEEHIKEEHNLWHYLFFIVLVRVKDSTEYTGPESYVAQMIKEHNLDWFPRMRAMSLVSSDGEGEQNELRSLQEKLESTMRLVSNLTNQLTELKEQMTEQRKHKQRLGLLGNPAHLNINPQQPA

Target ORF information:

RefSeq Version	XM_021474617.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474617.1

ORF Insert Sequence:

ATGATGACAT TACAAGGTGC TGGAGTTTTT GCCATGGTGG ACAAATTGTC TAGTTTCCTG 
CACATTGGGG ACGTTTGTTC CCTTTATGCT GAAGGCTCTA CTAGTGGCTT CATCAGCACT 
CTGGGGTTGG TTGATGACCG ATGTGTTGTT CAACCAGATG CCGGAGACCT GAATAACCCA 
CCTAAAAAGT TTCGGGACTG TCTGTTCAGA CTGTGTCCCA TGAACAGATA TTCGGCACAG 
AAACAGTTCT GGAAAGCTGC CAAACCTGGA GGAACTAGCA CCACAGATAC TGTCCTGCTT 
AACAAACTCC ATCATGCTGC TGATTTAGAG AAGAAACAAA ATGATTCTGA GAACAAAAAG 
CTGCTTGGAA CCGTCATTCA GTATGGCAAT GTCATCCAGC TTCTCCATCT GAAGAGTAAT 
AAATATCTGA CGGTGAATAA GCGTTTGCCG GCTCTTCTTG AGAAGAATGC CATGCGTGTG 
ACTCTGGACG CTGCAGGGAA TGAAGGCTCG TGGTTTTACA TCCAGCCCTT CTATAAGCTG 
CGCTCTATAG GGGATAATGT GGTCATTGGA GATAAGGTCG TGTTAAACCC TGTCAATGCC 
GGACAGCCGC TGCACGCCAG CAGCCATCAG CTTGTGGATA ATCCAGGCTG CAATGAAGTG 
AATTCAGTGA ACTGCACCAC CAGTTGGAAA ATCGTTCTCT TCATGAAATG GAGCGACAAC 
CAGGAGATTG TCTTGAAAGG AGGTGACGTG GTGAGGCTGT TTCATGCAGA GCAGGAGAAG 
TTTCTGACAT GTGATGAGCA TCGGAAAAAG CAGTATGTGT TTCTGCGTAC CACCGGACGC 
CAGTCTGCAA CCTCCGCCAC TAGTAGCAAA GCACTCTGGG AGGTAGAGGT GGTTCAGCAT 
GATCCCTGCC GAGGCGGAGC CGGCTACTGG AACAGCCTCT TTAGGTTTAA ACACTTAGCC 
ACCGGCCATT ACCTTGCTGC AGAGGTAAAT CCAGACTATG AAGAAGAATG CCTGGAGTCC 
CGCTCCTCTA TGGATTCGGA GCATGAAGTG ATTCGGGCTC GTGCACGTAA CCCTCAGGAT 
AAGGTGATGT ACACACTGGT GTCTGTTCCT GATGGAAATG ACATATCCTC CATCTTTGAG 
CTTGACCCTA CAACTTTGAG GGGCGGAGAC TCACTTGTGC CAAGAAACTC GTATGTGAGG 
CTCAGGCATC TCTGCACCAA CACGTGGGTC CACAGCACCA ACCAGCCAAT AGATAAAGAG 
GAAGAGAAAC CGGTCATGCT CAGAATAGGA ACGTCTCCTC TGAAGGAAGA TAAAGAGGCG 
TTTGCTATAG TACCAGTCTC TCCAGCTGAG GTTCGAGATC TGGATTTTGC TAATGATGCT 
AGTAAAGTGT TGGCCTCCAT TGCTGCCAAA CTGGAGAAGG GCACCATCAC CCAGAATGAG 
AGGAGGTCAG TGACGAAGCT GTTGGAGGAT CTGGTGTATT TTGTGGTGGA CATACCCAGC 
AGTGCTCAAG ATGTGCTGGA GATCACAGTC AACAAACCCA ACAGAGAGCG ACAGAAACTC 
ATGAGAGAGC AGAACATTCT TAAACAGATC TTCAAGCTGC TGCAGGCGCC ATTTACAGAC 
AGCGGGGACG GCCCTATGTT GCGATTAGAA GAGCTCGCAG ACCAGCGTCA TGCACCCTTT 
CGACACATTT GCAGACTCTG TTACAGAGTC CTACGTTACT CACAACAGGA CTATCGCAAA 
AATCAGGAGT ACATAGCAAA GCAGTTCCGC TTCATGCAGA AGCAGATAGG ATATGATGTG 
CTGGCTGAAG ACACAATTAC AGCCCTTCTG CACAACAACA GGAAGCTCCT AGAGAAACAC 
ATCACAGCAG CTGAAATAGA TACCTTTGTC AGTCTGGTCC GCAAGAACAG AGAGCCCAGA 
TTTCTGGATT ACTTGTCAGA TCTGTGTGTG TCGATGAATA AATCCATCCC GGTCACTCAA 
GAGCTCATCT GTAATGCTGT GCTGGACCCA GCCAATGCAG ACATTCTCAT CGAAACCAAG 
CTTGTGTTGT CACGATTTGA GGTGGCAGGA ACGGTGTTGG GTGAAGGTGC GGAGGAGGAA 
GAGGAGGATG AAGAGGAGGT CTGGCTGTTC TGGAAGGACA GTGGAGGAGA GGTGAAGAGT 
AAAAGCATAC GAGAGCTTGC ACAGGACGCA AAAGATGGAC ATACAGAGGA CCAGGAAGTC 
ATCAATTATT ACAGATATCA GCTGAATTTG TTTGCTCGGA TGTGTCTGGA TCGACAGTAT 
CTTGCGATTA ACAAGATATC AGCACAGCTG GATGTGGATC TGATTCTGCG CTGCATGTCA 
GATGAAGATT TGCCCTTTGA CCTCAGAGCA TCGTTCTGCC GCATGATGCT GCACATGCAT 
GTAGACCGTG ACCCTCAGGA GCAGGTCACC CCGGTCAAAT ATGCTCGACT GTGGTCTGAA 
ATCCCATCAC AAATTTCCAT TGATGATTAT GATAATGATG GGACGTCACG TGATGAAGTG 
AAAGAGCGCT TCTCTCAGAC GATGGAGTTT GTGGAGAATT ACCTGAGGGA TGTTGTCTGC 
CAGAGTTTCC CGTTCTCTGA TAAGGAGAAA AACAAACTCA CTTTTGAGGT GGTGAACCTG 
GCAAGAAACC TGATCTATTT TGGTTTCTAT AACTTCAGTG ATCTGCTAAG GTTAACAAAA 
ATCCTATTGG CAATCCTGGA CTGTGTCCAC ATAAGCACTC CCTTCCACAT CAAGCTGGAT 
AGAGAACCTG GTAAAGGAAG TAACGTGATG CGCTCCATCC ATGGGGTGGG AGAACTGATG 
ACACAGGTAG TGTTGAGGGG TGGGGGATTT CTGCCAGCCT CCAGCCACAA TCCTCCTGAC 
AGAGACGAAG TGAAAGCACA AAGTGAACCT CAGAAACAAG ACATACTGGT GATGGACACT 
AAACTCAAGA TCATCGAGAT CCTGCAGTTT ATTCTGAATG TGCGGTTGGA CTACAGGATC 
TCCTGCCTCC TCTGTATCTT TAAGACAGAG TTTGATGAAA GTAACTCTCA AAGTGAACCA 
TCTTTAAGCC CAGAATCTCC TGCCAGCGTT CAAGGTGCGC TAGACTTTGA GCACATTGAA 
GAGCAGGCGG AGGGTATTTT TGGAGGAAGT AGTGAAGAGA ACACCCCGCT GGATCTTGAT 
GATCATGGTG GACGGACATT TCTGAGGGTC CTGCTTCACC TGACCATGCA TGACTATCCT 
CCGCTGGTAT CTCGAGCTCT TCACCTGCTC TTCAGACACT TTAGCCAACG CCAGGAGGTC 
CTACAGGCCT TCAAACAGGT TCAGCTCCTG GTGACCAGTC AGGATGTGGA AAACTATAAA 
CAGATCAAGT CAGATTTGGA CCAGCTGAGG TCAATAGTAG AGAAATCAGA GTTGTGGGTG 
TATAAGAGAC AAGGCTCAGA CTCAGGACTG GATGCTGGAG AAGTCACCCC TGAAGCCCAC 
CACAAGGGTG TCATCAACTC GAGTAGATCA AACAAACCCA AAGTGGAAAG TACCAGCAGC 
TCGAACTACA GAGTCGTGAA AGAGATCCTG CTGAGGCTGA GCAAACTGTG TGTTTTGGAG 
GGCATCTCTG GCAAGAAGAA TAAGAAACAG CAGCAGCGGC TCCTCAGGAA CATGGGAGCT 
CACAGTGTGG TCCTCGAACT GTTGCAAATC CCTTATGAGA AGGGGGAGGA TGTCCAGATG 
CAGGAGATCA TGACCTTAGC ACATCAGTTC CTTCAAAACT TCTGTGCTGG AAATCAGCAG 
AACCAGGCAC TTCTACACAA ACACATCAAC CTGTTCCTCA ACCCTGGGAT CCTGGAGGCG 
GTGACCATGC AGCATATCTT CATGAATAAT TTCCAGTTGT GTAGTGAGAT TAATGAGCGG 
GTCGTGCAGC ATTTTGTGCA CTGCATCGAG ACACACGGCC GCAGCGTGCA CTACCTCAAG 
TTCCTGCAGA CCATTGTCAA GGCAGAGAAC AAGTTCATCA AGAAATGCCA AGACATCGTC 
ATGGCTGAGC TGGTGACTGC AGGTGAGGAT GTGCTGGTGT TCTATAATGA CCGGGCGTCT 
TTTCAGTCAT TGGTTCAGAT GATGCGTCTT GAGCGGGAAC GACTTGATGA GAGCAGTGCA 
CTCAGGTATC ACATTCACCT GGTGGAGTTG CTGGCTGTGT GCACTGAAGG CAAGAACGTC 
TACACTGAGA TCAAATGCAA CTCGCTGCTC CCTCTCGATG ATATTGTACG TGTGGTCACA 
CATGAGGACT GCATCCCTGA GGTGAAGATG GCCTACGTGA ACTTCCTGAA TCACTGTTAT 
GTTGATACAG AGGTTGAGAT GAAGGAGATC TACACCAGCA ATCACATGTG GAAACTTTTT 
GATGATTTTC TGGTGGACGT CTGCAGAGTG TGCAATAACA CAAGTGACCG TAAACACGCT 
GACACAGTTC TGGAGAGATA CGTTACAGAG ACCATAATGA GCATCGTCAC CACCTTTTTC 
AGCTCACCAT TCTCAGACCA GAGCACAAGT TTGCAGACCC GGCAGCCGGT GTTTGTGCAG 
CTGCTGCAGG GAGTGTTTCG TGTATATCAC TGCAACTGGC TTCTCCCAGG TCAGAAGGGG 
AATGTGGAAG CCTGCATCAA AGTGCTGAGT GACGTGGCCA AAGGCAGAGC CATAGCCATT 
CCTGTGGATC TGGACTGTCA GGTGAATAAT CTGTTCATGA AGTCCAACAA CATTGTGCAG 
AAAACAGCCC TCAGCTGGAG ACTCTCTGTC CGTAACGCGA CTCGGAGAGA CTCTGTTCTG 
ACCACCTCTC GAGACTATCG CAACATCATT GAAAGACTAC AGGATATAGT GTCTGCATTG 
GAGGAGCGGT TGAGGCCTCT GGTTCAGGCT GAGTTGTCAG TGTTGGTGGA TGTTCTCCAT 
CGCCCAGAGC TGCTGTTTCC AGAGCACACT GAAGCCCACC GCAAGTGTGA GAGTGGAGGC 
TTCATCTGCA AGTTGATAAA ACACACTAAG CAGCTTCTAG AAGAAAACGA AGAGAGGCTT 
TGCATCAAAG TCCTTCAGAC ACTCCGAGAG ATGATGACCA AAGACAGAGG ATATGGAGAG 
AAGGGGGAGG CCCTTCGGCA GCTGCTCGTC AATCGTTACT ATGGCTTCAG GAGTGGTGGG 
CGGAGAGAAA GTCTGACCTC GTTTGGTAAC TCCACCCTAA CTCCTGTCGG GCCAATCAAG 
AGCCAGCCAG GGGGGTTGAG CAGGGCTGAG ATGTCATTAA TGGAGGTGCA GTGTCATCTA 
GACCGTGAAG GGGCATCGGA TCTTGTCATT GATCTCATCA TGAACACTAC CAGCGACCGC 
GTCTTCCATG AAAGCATTCT GTTGGCCATC GCACTGTTGG AGGGCGGAAA CACCATCATA 
CAGCATTCCT TCTTCAAACG TTTAACAGAA GACAAAAATT CAGAGAAGTT TTTCAGGGTG 
TTTTATGACA GAATGAAAGT GGCTCAGGTG GAGATCAAAG CCACGGTTAC TGTCAACACT 
AGTGACCTCG GCAACAAGCG GCGTGACGAC AACACTCCAG ACAAAGATAC TCCACAACGC 
CGCAGAGGTA AAGACTCCGG TGTGGTGGTG ACAGATGATG CTCGTGAGCA GCTGTTGGAG 
GCTTCAGCTG CGACTAAGAA GGCCTTTGGT TCATATCGTC GAGATGCTGA TCCCGAGGAG 
ACGTTTGGCA CGGCTGATGG AGATAAAGGA GGTGGAGATA AAGGCACAGA GCAGGGCGAG 
ATGAGCCCTG TCATCCTCAT AATGCAGCCA ATCCTGCGCT TCCTGCAGCT GCTGTGCGAA 
AACCACAATC GAGACCTGCA GAATTTTCTG CGCTGTCAGA ACAATAAAAA CAACTATAAT 
CTGGTGTGTG AGACGCTGCA GTTCCTGGAC TGTATCTGTG GCAGCACAAC AGGAGGCCTG 
GGGCTGTTAG GACTCTACAT CAACCAGCAT AACGTAGCGC TCATTAATCA GACTGTAGAG 
AGCCTCACTG AATACTGTCA AGGTCCCTGC CATGACAATC AGAATTGCAT TGCCACCCAT 
GAGTCTAATG GCATCGACAT CATTATTGCA CTGATTCTGA ATGATATTAA TCCACTGGGC 
AGGAAGAGAA TGGATCTGGT TCTAGAACTA AAGAATAATG CATCTAAGCT GCTGCTGGCC 
ATCATGGAGA GCCGTCATGA CAGTGAGAAT GCAGAAAGAA TTCTGTACAA CATGAGACCA 
AAAGAACTAG TGGAGGTGAT GAAAATGGCC TACCAGCAGG GGGAGGCAGA GTTTGAGGAT 
GAGGAACAGG AGAATGGAGA GGATCATGCA GCATCGCCTC GCAATGTTGG ACACAACATC 
TATATTCTTG CACATCAGCT GTCGCGTCAC AATAAGGAAT TGCAAATACT ACTGAAACCA 
GGTGGAGAGG ACCAGGCCCT GGAGTATTAC ACAAAACACA CCTCACAGAT AGAGATAGTT 
CGACAGGATC GCACTATGGA ACAGATTGTG TTTCCTGTAC CAAATATCTG CTCTTTTCTG 
ACAAATGAGT CCAAGCTGCG TGTGTACTAT GGTACAGAGA GAGACGAGCA AGGCAGTAAA 
ATAAATGACT TCTTCTTGCA TGCAGACGAC CTCTTTAATG AAATGAGGTG GCAGAAAAAA 
CTAAGAGCTC AGCCAGTGCT GTACTGGTGC AGCAGGAACA TGTCGTTTTG GAGTAACGTT 
TCCTTTAATC TGGCGGTGCT CATTAATTTG CTGGTGGCAT TTTTCTACCC TCTGGATGGA 
GTGAGCGAAA GCCAGCTGGA ACCTTCTCTG TCTCTGTTGC TGTGGGTTTG TTTGTTGGGC 
TCACTGGGCT TCGTGTTGAT GTCCCCGAGG CCAAATGCAG TGCGGGTGCT GGTCATCTCC 
ACTGTCCTGC AGTTGGGCTT CTCTGTAGGG CTGCAGCACA TGCTCACACT CCTGGGAGCT 
TTCAATGTTT GTAATAAGAT CGTGTTCATG CTGAGCTTTG TGGGTAACCG TGGGTCATTC 
ACCCGGGGTT ATCGGGTGAT GGTCATGGAT AGAGAGTTCC TGTTTCATCT GCTGTACCTT 
CTCATCTGCA TACTGGGCCT TTTTGGACAC GTCTTCTTTT ACAGTCTTCT GTTGTTTGAC 
CTGGTGAACC GGGAAGAGAC TCTTCTGAAC GTCATCAAGA GTGTGACTCG AAACGGCCGC 
TCCATCGTCT TGACCGCAGT GCTGGGCCTC ATCCTGGTCT ACCTCTTTTC TATTGTTGGA 
TATATGTTTT TCAAAGATGA CTTCATCCTG GAGGTGAACC GCATCTCCAA CGGCACTCTG 
GAGGAGGGGG TGACTTTACC TGCTGGTTTT TGTGATGCGA GAAACGGGTC GTGTCTTTCT 
GATGATGTTG ACGAGGATGA CGTGGAGCGC GCGTGTGATT CTCTATGGAT GTGTATGATC 
ACTGTACTCA GTCATGGCCT CAGGAGCGGT GGAGGAGTTG GAGATGTCCT GCGCAAACCG 
TCTAAAGAGG AGCGTCTGTT CGCCGCACGG GTGGTGTACG ATCTCCTGTT CTTCTTCCTG 
GTGATCATCA TTGTGTTGAA TCTGATCTTC GGTGTGATCA TTGACACATT TGCAGATCTG 
AGAAGCGAGA AGCAGAGAAA AGAGGAGATC TTGAAGACCA CATGCTTCAT CTGTGGTCTG 
GAGAGAGATA AGTTTGATAA TAAAACGGTG ACGTTTGAAG AGCACATCAA GGAGGAGCAC 
AACCTGTGGC ATTACCTCTT CTTCATAGTG CTGGTGCGGG TCAAAGACTC CACCGAATAC 
ACGGGACCCG AGAGCTACGT CGCTCAGATG ATCAAGGAGC ACAATCTGGA CTGGTTTCCT 
CGTATGAGGG CGATGTCTCT GGTCAGCAGT GATGGAGAAG GTGAACAGAA TGAACTGCGC 
AGCTTACAGG AGAAACTCGA GTCCACAATG AGGCTCGTCT CTAATCTTAC CAATCAGCTC 
ACAGAGCTCA AAGAGCAGAT GACTGAGCAA AGGAAACACA AGCAGAGGCT CGGTCTGCTG 
GGAAACCCCG CCCACCTCAA CATCAACCCA CAGCAGCCAG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itpr1a ( XM_021474618.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330293.1 Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X14, mRNA.

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CloneID	ODa146877
Clone ID Related Accession (Same CDS sequence)	XM_021474618.1
Accession Version	XM_021474618.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395382.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACAT TACAAGGTGC TGGAGTTTTT GCCATGGTGG ACAAATTGTC TAGTTTCCTG 
CACATTGGGG ACGTTTGTTC CCTTTATGCT GAAGGCTCTA CTAGTGGCTT CATCAGCACT 
CTGGGGTTGG TTGATGACCG ATGTGTTGTT CAACCAGATG CCGGAGACCT GAATAACCCA 
CCTAAAAAGT TTCGGGACTG TCTGTTCAGA CTGTGTCCCA TGAACAGATA TTCGGCACAG 
AAACAGTTCT GGAAAGCTGC CAAACCTGGA GGAACTAGCA CCACAGATAC TGTCCTGCTT 
AACAAACTCC ATCATGCTGC TGATTTAGAG AAGAAACAAA ATGATTCTGA GAACAAAAAG 
CTGCTTGGAA CCGTCATTCA GTATGGCAAT GTCATCCAGC TTCTCCATCT GAAGAGTAAT 
AAATATCTGA CGGTGAATAA GCGTTTGCCG GCTCTTCTTG AGAAGAATGC CATGCGTGTG 
ACTCTGGACG CTGCAGGGAA TGAAGGCTCG TGGTTTTACA TCCAGCCCTT CTATAAGCTG 
CGCTCTATAG GGGATAATGT GGTCATTGGA GATAAGGTCG TGTTAAACCC TGTCAATGCC 
GGACAGCCGC TGCACGCCAG CAGCCATCAG CTTGTGGATA ATCCAGGCTG CAATGAAGTG 
AATTCAGTGA ACTGCACCAC CAGTTGGAAA ATCGTTCTCT TCATGAAATG GAGCGACAAC 
CAGGAGATTG TCTTGAAAGG AGGTGACGTG GTGAGGCTGT TTCATGCAGA GCAGGAGAAG 
TTTCTGACAT GTGATGAGCA TCGGAAAAAG CAGTATGTGT TTCTGCGTAC CACCGGACGC 
CAGTCTGCAA CCTCCGCCAC TAGTAGCAAA GCACTCTGGG AGGTAGAGGT GGTTCAGCAT 
GATCCCTGCC GAGGCGGAGC CGGCTACTGG AACAGCCTCT TTAGGTTTAA ACACTTAGCC 
ACCGGCCATT ACCTTGCTGC AGAGGTAAAT CCAGACTATG AAGAAGAATG CCTGGAGTCC 
CGCTCCTCTA TGGATTCGGA GCATGAAGTG ATTCGGGCTC GTGCACGTAA CCCTCAGGAT 
AAGGTGATGT ACACACTGGT GTCTGTTCCT GATGGAAATG ACATATCCTC CATCTTTGAG 
CTTGACCCTA CAACTTTGAG GGGCGGAGAC TCACTTGTGC CAAGAAACTC GTATGTGAGG 
CTCAGGCATC TCTGCACCAA CACGTGGGTC CACAGCACCA ACCAGCCAAT AGATAAAGAG 
GAAGAGAAAC CGGTCATGCT CAGAATAGGA ACGTCTCCTC TGAAGGAAGA TAAAGAGGCG 
TTTGCTATAG TACCAGTCTC TCCAGCTGAG GTTCGAGATC TGGATTTTGC TAATGATGCT 
AGTAAAGTGT TGGCCTCCAT TGCTGCCAAA CTGGAGAAGG GCACCATCAC CCAGAATGAG 
AGGAGGTCAG TGACGAAGCT GTTGGAGGAT CTGGTGTATT TTGTGGTGGA CATACCCAGC 
AGTGCTCAAG ATGTGCTGGA GATCACAGTC AACAAACCCA ACAGAGAGCG ACAGAAACTC 
ATGAGAGAGC AGAACATTCT TAAACAGATC TTCAAGCTGC TGCAGGCGCC ATTTACAGAC 
AGCGGGGACG GCCCTATGTT GCGATTAGAA GAGCTCGCAG ACCAGCGTCA TGCACCCTTT 
CGACACATTT GCAGACTCTG TTACAGAGTC CTACGTTACT CACAACAGGA CTATCGCAAA 
AATCAGGAGT ACATAGCAAA GCAGTTCCGC TTCATGCAGA AGCAGATAGG ATATGATGTG 
CTGGCTGAAG ACACAATTAC AGCCCTTCTG CACAACAACA GGAAGCTCCT AGAGAAACAC 
ATCACAGCAG CTGAAATAGA TACCTTTGTC AGTCTGGTCC GCAAGAACAG AGAGCCCAGA 
TTTCTGGATT ACTTGTCAGA TCTGTGTGTG TCGATGAATA AATCCATCCC GGTCACTCAA 
GAGCTCATCT GTAATGCTGT GCTGGACCCA GCCAATGCAG ACATTCTCAT CGAAACCAAG 
CTTGTGTTGT CACGATTTGA GGTGGCAGGA ACGGTGTTGG GTGAAGGTGC GGAGGAGGAA 
GAGGAGGATG AAGAGGAGGT CTGGCTGTTC TGGAAGGACA GTGGAGGAGA GGTGAAGAGT 
AAAAGCATAC GAGAGCTTGC ACAGGACGCA AAAGATGGAC ATACAGAGGA CCAGGAAGTC 
ATCAATTATT ACAGATATCA GCTGAATTTG TTTGCTCGGA TGTGTCTGGA TCGACAGTAT 
CTTGCGATTA ACAAGATATC AGCACAGCTG GATGTGGATC TGATTCTGCG CTGCATGTCA 
GATGAAGATT TGCCCTTTGA CCTCAGAGCA TCGTTCTGCC GCATGATGCT GCACATGCAT 
GTAGACCGTG ACCCTCAGGA GCAGGTCACC CCGGTCAAAT ATGCTCGACT GTGGTCTGAA 
ATCCCATCAC AAATTTCCAT TGATGATTAT GATAATGATG GGACGTCACG TGATGAAGTG 
AAAGAGCGCT TCTCTCAGAC GATGGAGTTT GTGGAGAATT ACCTGAGGGA TGTTGTCTGC 
CAGAGTTTCC CGTTCTCTGA TAAGGAGAAA AACAAACTCA CTTTTGAGGT GGTGAACCTG 
GCAAGAAACC TGATCTATTT TGGTTTCTAT AACTTCAGTG ATCTGCTAAG GTTAACAAAA 
ATCCTATTGG CAATCCTGGA CTGTGTCCAC ATAAGCACTC CCTTCCACAT CAAGCTGGAT 
AGAGAACCTG GTAAAGGAAG TAACGTGATG CGCTCCATCC ATGGGGTGGG AGAACTGATG 
ACACAGGTAG TGTTGAGGGG TGGGGGATTT CTGCCAGCCT CCAGCCACAA TCCTCCTGAC 
AGAGACGAAG TGAAAGCACA AAGTGAACCT CAGAAACAAG ACATACTGGT GATGGACACT 
AAACTCAAGA TCATCGAGAT CCTGCAGTTT ATTCTGAATG TGCGGTTGGA CTACAGGATC 
TCCTGCCTCC TCTGTATCTT TAAGACAGAG TTTGATGAAA GTAACTCTCA AAGTGAACCA 
TCTTTAAGCC CAGAATCTCC TGCCAGCGTT CAAGGTGCGC TAGACTTTGA GCACATTGAA 
GAGCAGGCGG AGGGTATTTT TGGAGGAAGT GAAGAGAACA CCCCGCTGGA TCTTGATGAT 
CATGGTGGAC GGACATTTCT GAGGGTCCTG CTTCACCTGA CCATGCATGA CTATCCTCCG 
CTGGTATCTC GAGCTCTTCA CCTGCTCTTC AGACACTTTA GCCAACGCCA GGAGGTCCTA 
CAGGCCTTCA AACAGGTTCA GCTCCTGGTG ACCAGTCAGG ATGTGGAAAA CTATAAACAG 
ATCAAGTCAG ATTTGGACCA GCTGAGGTCA ATAGTAGAGA AATCAGAGTT GTGGGTGTAT 
AAGAGACAAG GCTCAGACTC AGGACTGGAT GCTGGAGAAG TCACCCCTGA AGCCCACCAC 
AAGGGTGTCA TCAACTCGAG TAGATCAAAC AAACCCAAAG TGGAAAGTAC CAGCAGCTCG 
AACTACAGAG TCGTGAAAGA GATCCTGCTG AGGCTGAGCA AACTGTGTGT TTTGGAGGGC 
ATCTCTGGCA AGAAGAATAA GAAACAGCAG CAGCGGCTCC TCAGGAACAT GGGAGCTCAC 
AGTGTGGTCC TCGAACTGTT GCAAATCCCT TATGAGAAGG GGGAGGATGT CCAGATGCAG 
GAGATCATGA CCTTAGCACA TCAGTTCCTT CAAAACTTCT GTGCTGGAAA TCAGCAGAAC 
CAGGCACTTC TACACAAACA CATCAACCTG TTCCTCAACC CTGGGATCCT GGAGGCGGTG 
ACCATGCAGC ATATCTTCAT GAATAATTTC CAGTTGTGTA GTGAGATTAA TGAGCGGGTC 
GTGCAGCATT TTGTGCACTG CATCGAGACA CACGGCCGCA GCGTGCACTA CCTCAAGTTC 
CTGCAGACCA TTGTCAAGGC AGAGAACAAG TTCATCAAGA AATGCCAAGA CATCGTCATG 
GCTGAGCTGG TGACTGCAGG TGAGGATGTG CTGGTGTTCT ATAATGACCG GGCGTCTTTT 
CAGTCATTGG TTCAGATGAT GCGTCTTGAG CGGGAACGAC TTGATGAGAG CAGTGCACTC 
AGGTATCACA TTCACCTGGT GGAGTTGCTG GCTGTGTGCA CTGAAGGCAA GAACGTCTAC 
ACTGAGATCA AATGCAACTC GCTGCTCCCT CTCGATGATA TTGTACGTGT GGTCACACAT 
GAGGACTGCA TCCCTGAGGT GAAGATGGCC TACGTGAACT TCCTGAATCA CTGTTATGTT 
GATACAGAGG TTGAGATGAA GGAGATCTAC ACCAGCAATC ACATGTGGAA ACTTTTTGAT 
GATTTTCTGG TGGACGTCTG CAGAGTGTGC AATAACACAA GTGACCGTAA ACACGCTGAC 
ACAGTTCTGG AGAGATACGT TACAGAGACC ATAATGAGCA TCGTCACCAC CTTTTTCAGC 
TCACCATTCT CAGACCAGAG CACAAGTTTG CAGACCCGGC AGCCGGTGTT TGTGCAGCTG 
CTGCAGGGAG TGTTTCGTGT ATATCACTGC AACTGGCTTC TCCCAGGTCA GAAGGGGAAT 
GTGGAAGCCT GCATCAAAGT GCTGAGTGAC GTGGCCAAAG GCAGAGCCAT AGCCATTCCT 
GTGGATCTGG ACTGTCAGGT GAATAATCTG TTCATGAAGT CCAACAACAT TGTGCAGAAA 
ACAGCCCTCA GCTGGAGACT CTCTGTCCGT AACGCGACTC GGAGAGACTC TGTTCTGACC 
ACCTCTCGAG ACTATCGCAA CATCATTGAA AGACTACAGG ATATAGTGTC TGCATTGGAG 
GAGCGGTTGA GGCCTCTGGT TCAGGCTGAG TTGTCAGTGT TGGTGGATGT TCTCCATCGC 
CCAGAGCTGC TGTTTCCAGA GCACACTGAA GCCCACCGCA AGTGTGAGAG TGGAGGCTTC 
ATCTGCAAGT TGATAAAACA CACTAAGCAG CTTCTAGAAG AAAACGAAGA GAGGCTTTGC 
ATCAAAGTCC TTCAGACACT CCGAGAGATG ATGACCAAAG ACAGAGGATA TGGAGAGAAG 
GGGGAGGCCC TTCGGCAGCT GCTCGTCAAT CGTTACTATG GCTTCAGGAG TGGTGGGCGG 
AGAGAAAGTC TGACCTCGTT TGGTAACTCC ACCCTAACTC CTGTCGGGCC AATCAAGAGC 
CAGCCAGGGG GGTTGAGCAG GGCTGAGATG TCATTAATGG AGGTGCAGTG TCATCTAGAC 
CGTGAAGGGG CATCGGATCT TGTCATTGAT CTCATCATGA ACACTACCAG CGACCGCGTC 
TTCCATGAAA GCATTCTGTT GGCCATCGCA CTGTTGGAGG GCGGAAACAC CATCATACAG 
CATTCCTTCT TCAAACGTTT AACAGAAGAC AAAAATTCAG AGAAGTTTTT CAGGGTGTTT 
TATGACAGAA TGAAAGTGGC TCAGGTGGAG ATCAAAGCCA CGGTTACTGT CAACACTAGT 
GACCTCGGCA ACAAGCGGCG TGACGACAAC ACTCCAGACA AAGATACTCC ACAACGCCGC 
AGAGGTAAAG ACTCCGGTGT GGTGGTGACA GATGATGCTC GTGAGCAGCT GTTGGAGGCT 
TCAGCTGCGA CTAAGAAGGC CTTTGGTTCA TATCGTCGAG ATGCTGATCC CGAGGAGACG 
TTTGGCACGG CTGATGGAGA TAAAGGAGGT GGAGATAAAG GCACAGAGCA GGGCGAGATG 
AGCCCTGTCA TCCTCATAAT GCAGCCAATC CTGCGCTTCC TGCAGCTGCT GTGCGAAAAC 
CACAATCGAG ACCTGCAGAA TTTTCTGCGC TGTCAGAACA ATAAAAACAA CTATAATCTG 
GTGTGTGAGA CGCTGCAGTT CCTGGACTGT ATCTGTGGCA GCACAACAGG AGGCCTGGGG 
CTGTTAGGAC TCTACATCAA CCAGCATAAC GTAGCGCTCA TTAATCAGAC TGTAGAGAGC 
CTCACTGAAT ACTGTCAAGG TCCCTGCCAT GACAATCAGA ATTGCATTGC CACCCATGAG 
TCTAATGGCA TCGACATCAT TATTGCACTG ATTCTGAATG ATATTAATCC ACTGGGCAGG 
AAGAGAATGG ATCTGGTTCT AGAACTAAAG AATAATGCAT CTAAGCTGCT GCTGGCCATC 
ATGGAGAGCC GTCATGACAG TGAGAATGCA GAAAGAATTC TGTACAACAT GAGACCAAAA 
GAACTAGTGG AGGTGATGAA AATGGCCTAC CAGCAGGGGG AGGCAGAGTT TGAGGATGAG 
GAACAGGAGA ATGGAGAGGA TCATGCAGCA TCGCCTCGCA ATGTTGGACA CAACATCTAT 
ATTCTTGCAC ATCAGCTGTC GCGTCACAAT AAGGAATTGC AAATACTACT GAAACCAGGT 
GGAGAGGACC AGGCCCTGGA GTATTACACA AAACACACCT CACAGATAGA GATAGTTCGA 
CAGGATCGCA CTATGGAACA GATTGTGTTT CCTGTACCAA ATATCTGCTC TTTTCTGACA 
AATGAGTCCA AGCTGCGTGT GTACTATGGT ACAGAGAGAG ACGAGCAAGG CAGTAAAATA 
AATGACTTCT TCTTGCATGC AGACGACCTC TTTAATGAAA TGAGGTGGCA GAAAAAACTA 
AGAGCTCAGC CAGTGCTGTA CTGGTGCAGC AGGAACATGT CGTTTTGGAG TAACGTTTCC 
TTTAATCTGG CGGTGCTCAT TAATTTGCTG GTGGCATTTT TCTACCCTCT GGATGGAGTG 
AGCGAAAGCC AGCTGGAACC TTCTCTGTCT CTGTTGCTGT GGGTTTGTTT GTTGGGCTCA 
CTGGGCTTCG TGTTGATGTC CCCGAGGCCA AATGCAGTGC GGGTGCTGGT CATCTCCACT 
GTCCTGCAGT TGGGCTTCTC TGTAGGGCTG CAGCACATGC TCACACTCCT GGGAGCTTTC 
AATGTTTGTA ATAAGATCGT GTTCATGCTG AGCTTTGTGG GTAACCGTGG GTCATTCACC 
CGGGGTTATC GGGTGATGGT CATGGATAGA GAGTTCCTGT TTCATCTGCT GTACCTTCTC 
ATCTGCATAC TGGGCCTTTT TGGACACGTC TTCTTTTACA GTCTTCTGTT GTTTGACCTG 
GTGAACCGGG AAGAGACTCT TCTGAACGTC ATCAAGAGTG TGACTCGAAA CGGCCGCTCC 
ATCGTCTTGA CCGCAGTGCT GGGCCTCATC CTGGTCTACC TCTTTTCTAT TGTTGGATAT 
ATGTTTTTCA AAGATGACTT CATCCTGGAG GTGAACCGCA TCTCCAACGG CACTCTGGAG 
GAGGGGGTGA CTTTACCTGC TGGTTTTTGT GATGCGAGAA ACGGGTCGTG TCTTTCTGAT 
GATGTTGACG AGGATGACGT GGAGCGCGCG TGTGATTCTC TATGGATGTG TATGATCACT 
GTACTCAGTC ATGGCCTCAG GAGCGGTGGA GGAGTTGGAG ATGTCCTGCG CAAACCGTCT 
AAAGAGGAGC GTCTGTTCGC CGCACGGGTG GTGTACGATC TCCTGTTCTT CTTCCTGGTG 
ATCATCATTG TGTTGAATCT GATCTTCGGT GTGATCATTG ACACATTTGC AGATCTGAGA 
AGCGAGAAGC AGAGAAAAGA GGAGATCTTG AAGACCACAT GCTTCATCTG TGGTCTGGAG 
AGAGATAAGT TTGATAATAA AACGGTGACG TTTGAAGAGC ACATCAAGGA GGAGCACAAC 
CTGTGGCATT ACCTCTTCTT CATAGTGCTG GTGCGGGTCA AAGACTCCAC CGAATACACG 
GGACCCGAGA GCTACGTCGC TCAGATGATC AAGGAGCACA ATCTGGACTG GTTTCCTCGT 
ATGAGGGCGA TGTCTCTGGT CAGCAGTGAT GGAGAAGGTG AACAGAATGA ACTGCGCAGC 
TTACAGGAGA AACTCGAGTC CACAATGAGG CTCGTCTCTA ATCTTACCAA TCAGCTCACA 
GAGCTCAAAG AGCAGATGAC TGAGCAAAGG AAACACAAGC AGAGGCTCGG TCTGCTGGGA 
AACCCCGCCC ACCTCAACAT CAACCCACAG CAGCCAGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330293.1
CDS	265..8346
Translation	MMTLQGAGVFAMVDKLSSFLHIGDVCSLYAEGSTSGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGTSTTDTVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDAAGNEGSWFYIQPFYKLRSIGDNVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCTTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDEHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEVNPDYEEECLESRSSMDSEHEVIRARARNPQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSPLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAAKLEKGTITQNERRSVTKLLEDLVYFVVDIPSSAQDVLEITVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRYSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDPANADILIETKLVLSRFEVAGTVLGEGAEEEEEDEEEVWLFWKDSGGEVKSKSIRELAQDAKDGHTEDQEVINYYRYQLNLFARMCLDRQYLAINKISAQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSQISIDDYDNDGTSRDEVKERFSQTMEFVENYLRDVVCQSFPFSDKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHISTPFHIKLDREPGKGSNVMRSIHGVGELMTQVVLRGGGFLPASSHNPPDRDEVKAQSEPQKQDILVMDTKLKIIEILQFILNVRLDYRISCLLCIFKTEFDESNSQSEPSLSPESPASVQGALDFEHIEEQAEGIFGGSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALHLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSDSGLDAGEVTPEAHHKGVINSSRSNKPKVESTSSSNYRVVKEILLRLSKLCVLEGISGKKNKKQQQRLLRNMGAHSVVLELLQIPYEKGEDVQMQEIMTLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAVTMQHIFMNNFQLCSEINERVVQHFVHCIETHGRSVHYLKFLQTIVKAENKFIKKCQDIVMAELVTAGEDVLVFYNDRASFQSLVQMMRLERERLDESSALRYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKMAYVNFLNHCYVDTEVEMKEIYTSNHMWKLFDDFLVDVCRVCNNTSDRKHADTVLERYVTETIMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQGVFRVYHCNWLLPGQKGNVEACIKVLSDVAKGRAIAIPVDLDCQVNNLFMKSNNIVQKTALSWRLSVRNATRRDSVLTTSRDYRNIIERLQDIVSALEERLRPLVQAELSVLVDVLHRPELLFPEHTEAHRKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKGEALRQLLVNRYYGFRSGGRRESLTSFGNSTLTPVGPIKSQPGGLSRAEMSLMEVQCHLDREGASDLVIDLIMNTTSDRVFHESILLAIALLEGGNTIIQHSFFKRLTEDKNSEKFFRVFYDRMKVAQVEIKATVTVNTSDLGNKRRDDNTPDKDTPQRRRGKDSGVVVTDDAREQLLEASAATKKAFGSYRRDADPEETFGTADGDKGGGDKGTEQGEMSPVILIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINQHNVALINQTVESLTEYCQGPCHDNQNCIATHESNGIDIIIALILNDINPLGRKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVMKMAYQQGEAEFEDEEQENGEDHAASPRNVGHNIYILAHQLSRHNKELQILLKPGGEDQALEYYTKHTSQIEIVRQDRTMEQIVFPVPNICSFLTNESKLRVYYGTERDEQGSKINDFFLHADDLFNEMRWQKKLRAQPVLYWCSRNMSFWSNVSFNLAVLINLLVAFFYPLDGVSESQLEPSLSLLLWVCLLGSLGFVLMSPRPNAVRVLVISTVLQLGFSVGLQHMLTLLGAFNVCNKIVFMLSFVGNRGSFTRGYRVMVMDREFLFHLLYLLICILGLFGHVFFYSLLLFDLVNREETLLNVIKSVTRNGRSIVLTAVLGLILVYLFSIVGYMFFKDDFILEVNRISNGTLEEGVTLPAGFCDARNGSCLSDDVDEDDVERACDSLWMCMITVLSHGLRSGGGVGDVLRKPSKEERLFAARVVYDLLFFFLVIIIVLNLIFGVIIDTFADLRSEKQRKEEILKTTCFICGLERDKFDNKTVTFEEHIKEEHNLWHYLFFIVLVRVKDSTEYTGPESYVAQMIKEHNLDWFPRMRAMSLVSSDGEGEQNELRSLQEKLESTMRLVSNLTNQLTELKEQMTEQRKHKQRLGLLGNPAHLNINPQQPA

Target ORF information:

RefSeq Version	XM_021474618.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474618.1

ORF Insert Sequence:

ATGATGACAT TACAAGGTGC TGGAGTTTTT GCCATGGTGG ACAAATTGTC TAGTTTCCTG 
CACATTGGGG ACGTTTGTTC CCTTTATGCT GAAGGCTCTA CTAGTGGCTT CATCAGCACT 
CTGGGGTTGG TTGATGACCG ATGTGTTGTT CAACCAGATG CCGGAGACCT GAATAACCCA 
CCTAAAAAGT TTCGGGACTG TCTGTTCAGA CTGTGTCCCA TGAACAGATA TTCGGCACAG 
AAACAGTTCT GGAAAGCTGC CAAACCTGGA GGAACTAGCA CCACAGATAC TGTCCTGCTT 
AACAAACTCC ATCATGCTGC TGATTTAGAG AAGAAACAAA ATGATTCTGA GAACAAAAAG 
CTGCTTGGAA CCGTCATTCA GTATGGCAAT GTCATCCAGC TTCTCCATCT GAAGAGTAAT 
AAATATCTGA CGGTGAATAA GCGTTTGCCG GCTCTTCTTG AGAAGAATGC CATGCGTGTG 
ACTCTGGACG CTGCAGGGAA TGAAGGCTCG TGGTTTTACA TCCAGCCCTT CTATAAGCTG 
CGCTCTATAG GGGATAATGT GGTCATTGGA GATAAGGTCG TGTTAAACCC TGTCAATGCC 
GGACAGCCGC TGCACGCCAG CAGCCATCAG CTTGTGGATA ATCCAGGCTG CAATGAAGTG 
AATTCAGTGA ACTGCACCAC CAGTTGGAAA ATCGTTCTCT TCATGAAATG GAGCGACAAC 
CAGGAGATTG TCTTGAAAGG AGGTGACGTG GTGAGGCTGT TTCATGCAGA GCAGGAGAAG 
TTTCTGACAT GTGATGAGCA TCGGAAAAAG CAGTATGTGT TTCTGCGTAC CACCGGACGC 
CAGTCTGCAA CCTCCGCCAC TAGTAGCAAA GCACTCTGGG AGGTAGAGGT GGTTCAGCAT 
GATCCCTGCC GAGGCGGAGC CGGCTACTGG AACAGCCTCT TTAGGTTTAA ACACTTAGCC 
ACCGGCCATT ACCTTGCTGC AGAGGTAAAT CCAGACTATG AAGAAGAATG CCTGGAGTCC 
CGCTCCTCTA TGGATTCGGA GCATGAAGTG ATTCGGGCTC GTGCACGTAA CCCTCAGGAT 
AAGGTGATGT ACACACTGGT GTCTGTTCCT GATGGAAATG ACATATCCTC CATCTTTGAG 
CTTGACCCTA CAACTTTGAG GGGCGGAGAC TCACTTGTGC CAAGAAACTC GTATGTGAGG 
CTCAGGCATC TCTGCACCAA CACGTGGGTC CACAGCACCA ACCAGCCAAT AGATAAAGAG 
GAAGAGAAAC CGGTCATGCT CAGAATAGGA ACGTCTCCTC TGAAGGAAGA TAAAGAGGCG 
TTTGCTATAG TACCAGTCTC TCCAGCTGAG GTTCGAGATC TGGATTTTGC TAATGATGCT 
AGTAAAGTGT TGGCCTCCAT TGCTGCCAAA CTGGAGAAGG GCACCATCAC CCAGAATGAG 
AGGAGGTCAG TGACGAAGCT GTTGGAGGAT CTGGTGTATT TTGTGGTGGA CATACCCAGC 
AGTGCTCAAG ATGTGCTGGA GATCACAGTC AACAAACCCA ACAGAGAGCG ACAGAAACTC 
ATGAGAGAGC AGAACATTCT TAAACAGATC TTCAAGCTGC TGCAGGCGCC ATTTACAGAC 
AGCGGGGACG GCCCTATGTT GCGATTAGAA GAGCTCGCAG ACCAGCGTCA TGCACCCTTT 
CGACACATTT GCAGACTCTG TTACAGAGTC CTACGTTACT CACAACAGGA CTATCGCAAA 
AATCAGGAGT ACATAGCAAA GCAGTTCCGC TTCATGCAGA AGCAGATAGG ATATGATGTG 
CTGGCTGAAG ACACAATTAC AGCCCTTCTG CACAACAACA GGAAGCTCCT AGAGAAACAC 
ATCACAGCAG CTGAAATAGA TACCTTTGTC AGTCTGGTCC GCAAGAACAG AGAGCCCAGA 
TTTCTGGATT ACTTGTCAGA TCTGTGTGTG TCGATGAATA AATCCATCCC GGTCACTCAA 
GAGCTCATCT GTAATGCTGT GCTGGACCCA GCCAATGCAG ACATTCTCAT CGAAACCAAG 
CTTGTGTTGT CACGATTTGA GGTGGCAGGA ACGGTGTTGG GTGAAGGTGC GGAGGAGGAA 
GAGGAGGATG AAGAGGAGGT CTGGCTGTTC TGGAAGGACA GTGGAGGAGA GGTGAAGAGT 
AAAAGCATAC GAGAGCTTGC ACAGGACGCA AAAGATGGAC ATACAGAGGA CCAGGAAGTC 
ATCAATTATT ACAGATATCA GCTGAATTTG TTTGCTCGGA TGTGTCTGGA TCGACAGTAT 
CTTGCGATTA ACAAGATATC AGCACAGCTG GATGTGGATC TGATTCTGCG CTGCATGTCA 
GATGAAGATT TGCCCTTTGA CCTCAGAGCA TCGTTCTGCC GCATGATGCT GCACATGCAT 
GTAGACCGTG ACCCTCAGGA GCAGGTCACC CCGGTCAAAT ATGCTCGACT GTGGTCTGAA 
ATCCCATCAC AAATTTCCAT TGATGATTAT GATAATGATG GGACGTCACG TGATGAAGTG 
AAAGAGCGCT TCTCTCAGAC GATGGAGTTT GTGGAGAATT ACCTGAGGGA TGTTGTCTGC 
CAGAGTTTCC CGTTCTCTGA TAAGGAGAAA AACAAACTCA CTTTTGAGGT GGTGAACCTG 
GCAAGAAACC TGATCTATTT TGGTTTCTAT AACTTCAGTG ATCTGCTAAG GTTAACAAAA 
ATCCTATTGG CAATCCTGGA CTGTGTCCAC ATAAGCACTC CCTTCCACAT CAAGCTGGAT 
AGAGAACCTG GTAAAGGAAG TAACGTGATG CGCTCCATCC ATGGGGTGGG AGAACTGATG 
ACACAGGTAG TGTTGAGGGG TGGGGGATTT CTGCCAGCCT CCAGCCACAA TCCTCCTGAC 
AGAGACGAAG TGAAAGCACA AAGTGAACCT CAGAAACAAG ACATACTGGT GATGGACACT 
AAACTCAAGA TCATCGAGAT CCTGCAGTTT ATTCTGAATG TGCGGTTGGA CTACAGGATC 
TCCTGCCTCC TCTGTATCTT TAAGACAGAG TTTGATGAAA GTAACTCTCA AAGTGAACCA 
TCTTTAAGCC CAGAATCTCC TGCCAGCGTT CAAGGTGCGC TAGACTTTGA GCACATTGAA 
GAGCAGGCGG AGGGTATTTT TGGAGGAAGT GAAGAGAACA CCCCGCTGGA TCTTGATGAT 
CATGGTGGAC GGACATTTCT GAGGGTCCTG CTTCACCTGA CCATGCATGA CTATCCTCCG 
CTGGTATCTC GAGCTCTTCA CCTGCTCTTC AGACACTTTA GCCAACGCCA GGAGGTCCTA 
CAGGCCTTCA AACAGGTTCA GCTCCTGGTG ACCAGTCAGG ATGTGGAAAA CTATAAACAG 
ATCAAGTCAG ATTTGGACCA GCTGAGGTCA ATAGTAGAGA AATCAGAGTT GTGGGTGTAT 
AAGAGACAAG GCTCAGACTC AGGACTGGAT GCTGGAGAAG TCACCCCTGA AGCCCACCAC 
AAGGGTGTCA TCAACTCGAG TAGATCAAAC AAACCCAAAG TGGAAAGTAC CAGCAGCTCG 
AACTACAGAG TCGTGAAAGA GATCCTGCTG AGGCTGAGCA AACTGTGTGT TTTGGAGGGC 
ATCTCTGGCA AGAAGAATAA GAAACAGCAG CAGCGGCTCC TCAGGAACAT GGGAGCTCAC 
AGTGTGGTCC TCGAACTGTT GCAAATCCCT TATGAGAAGG GGGAGGATGT CCAGATGCAG 
GAGATCATGA CCTTAGCACA TCAGTTCCTT CAAAACTTCT GTGCTGGAAA TCAGCAGAAC 
CAGGCACTTC TACACAAACA CATCAACCTG TTCCTCAACC CTGGGATCCT GGAGGCGGTG 
ACCATGCAGC ATATCTTCAT GAATAATTTC CAGTTGTGTA GTGAGATTAA TGAGCGGGTC 
GTGCAGCATT TTGTGCACTG CATCGAGACA CACGGCCGCA GCGTGCACTA CCTCAAGTTC 
CTGCAGACCA TTGTCAAGGC AGAGAACAAG TTCATCAAGA AATGCCAAGA CATCGTCATG 
GCTGAGCTGG TGACTGCAGG TGAGGATGTG CTGGTGTTCT ATAATGACCG GGCGTCTTTT 
CAGTCATTGG TTCAGATGAT GCGTCTTGAG CGGGAACGAC TTGATGAGAG CAGTGCACTC 
AGGTATCACA TTCACCTGGT GGAGTTGCTG GCTGTGTGCA CTGAAGGCAA GAACGTCTAC 
ACTGAGATCA AATGCAACTC GCTGCTCCCT CTCGATGATA TTGTACGTGT GGTCACACAT 
GAGGACTGCA TCCCTGAGGT GAAGATGGCC TACGTGAACT TCCTGAATCA CTGTTATGTT 
GATACAGAGG TTGAGATGAA GGAGATCTAC ACCAGCAATC ACATGTGGAA ACTTTTTGAT 
GATTTTCTGG TGGACGTCTG CAGAGTGTGC AATAACACAA GTGACCGTAA ACACGCTGAC 
ACAGTTCTGG AGAGATACGT TACAGAGACC ATAATGAGCA TCGTCACCAC CTTTTTCAGC 
TCACCATTCT CAGACCAGAG CACAAGTTTG CAGACCCGGC AGCCGGTGTT TGTGCAGCTG 
CTGCAGGGAG TGTTTCGTGT ATATCACTGC AACTGGCTTC TCCCAGGTCA GAAGGGGAAT 
GTGGAAGCCT GCATCAAAGT GCTGAGTGAC GTGGCCAAAG GCAGAGCCAT AGCCATTCCT 
GTGGATCTGG ACTGTCAGGT GAATAATCTG TTCATGAAGT CCAACAACAT TGTGCAGAAA 
ACAGCCCTCA GCTGGAGACT CTCTGTCCGT AACGCGACTC GGAGAGACTC TGTTCTGACC 
ACCTCTCGAG ACTATCGCAA CATCATTGAA AGACTACAGG ATATAGTGTC TGCATTGGAG 
GAGCGGTTGA GGCCTCTGGT TCAGGCTGAG TTGTCAGTGT TGGTGGATGT TCTCCATCGC 
CCAGAGCTGC TGTTTCCAGA GCACACTGAA GCCCACCGCA AGTGTGAGAG TGGAGGCTTC 
ATCTGCAAGT TGATAAAACA CACTAAGCAG CTTCTAGAAG AAAACGAAGA GAGGCTTTGC 
ATCAAAGTCC TTCAGACACT CCGAGAGATG ATGACCAAAG ACAGAGGATA TGGAGAGAAG 
GGGGAGGCCC TTCGGCAGCT GCTCGTCAAT CGTTACTATG GCTTCAGGAG TGGTGGGCGG 
AGAGAAAGTC TGACCTCGTT TGGTAACTCC ACCCTAACTC CTGTCGGGCC AATCAAGAGC 
CAGCCAGGGG GGTTGAGCAG GGCTGAGATG TCATTAATGG AGGTGCAGTG TCATCTAGAC 
CGTGAAGGGG CATCGGATCT TGTCATTGAT CTCATCATGA ACACTACCAG CGACCGCGTC 
TTCCATGAAA GCATTCTGTT GGCCATCGCA CTGTTGGAGG GCGGAAACAC CATCATACAG 
CATTCCTTCT TCAAACGTTT AACAGAAGAC AAAAATTCAG AGAAGTTTTT CAGGGTGTTT 
TATGACAGAA TGAAAGTGGC TCAGGTGGAG ATCAAAGCCA CGGTTACTGT CAACACTAGT 
GACCTCGGCA ACAAGCGGCG TGACGACAAC ACTCCAGACA AAGATACTCC ACAACGCCGC 
AGAGGTAAAG ACTCCGGTGT GGTGGTGACA GATGATGCTC GTGAGCAGCT GTTGGAGGCT 
TCAGCTGCGA CTAAGAAGGC CTTTGGTTCA TATCGTCGAG ATGCTGATCC CGAGGAGACG 
TTTGGCACGG CTGATGGAGA TAAAGGAGGT GGAGATAAAG GCACAGAGCA GGGCGAGATG 
AGCCCTGTCA TCCTCATAAT GCAGCCAATC CTGCGCTTCC TGCAGCTGCT GTGCGAAAAC 
CACAATCGAG ACCTGCAGAA TTTTCTGCGC TGTCAGAACA ATAAAAACAA CTATAATCTG 
GTGTGTGAGA CGCTGCAGTT CCTGGACTGT ATCTGTGGCA GCACAACAGG AGGCCTGGGG 
CTGTTAGGAC TCTACATCAA CCAGCATAAC GTAGCGCTCA TTAATCAGAC TGTAGAGAGC 
CTCACTGAAT ACTGTCAAGG TCCCTGCCAT GACAATCAGA ATTGCATTGC CACCCATGAG 
TCTAATGGCA TCGACATCAT TATTGCACTG ATTCTGAATG ATATTAATCC ACTGGGCAGG 
AAGAGAATGG ATCTGGTTCT AGAACTAAAG AATAATGCAT CTAAGCTGCT GCTGGCCATC 
ATGGAGAGCC GTCATGACAG TGAGAATGCA GAAAGAATTC TGTACAACAT GAGACCAAAA 
GAACTAGTGG AGGTGATGAA AATGGCCTAC CAGCAGGGGG AGGCAGAGTT TGAGGATGAG 
GAACAGGAGA ATGGAGAGGA TCATGCAGCA TCGCCTCGCA ATGTTGGACA CAACATCTAT 
ATTCTTGCAC ATCAGCTGTC GCGTCACAAT AAGGAATTGC AAATACTACT GAAACCAGGT 
GGAGAGGACC AGGCCCTGGA GTATTACACA AAACACACCT CACAGATAGA GATAGTTCGA 
CAGGATCGCA CTATGGAACA GATTGTGTTT CCTGTACCAA ATATCTGCTC TTTTCTGACA 
AATGAGTCCA AGCTGCGTGT GTACTATGGT ACAGAGAGAG ACGAGCAAGG CAGTAAAATA 
AATGACTTCT TCTTGCATGC AGACGACCTC TTTAATGAAA TGAGGTGGCA GAAAAAACTA 
AGAGCTCAGC CAGTGCTGTA CTGGTGCAGC AGGAACATGT CGTTTTGGAG TAACGTTTCC 
TTTAATCTGG CGGTGCTCAT TAATTTGCTG GTGGCATTTT TCTACCCTCT GGATGGAGTG 
AGCGAAAGCC AGCTGGAACC TTCTCTGTCT CTGTTGCTGT GGGTTTGTTT GTTGGGCTCA 
CTGGGCTTCG TGTTGATGTC CCCGAGGCCA AATGCAGTGC GGGTGCTGGT CATCTCCACT 
GTCCTGCAGT TGGGCTTCTC TGTAGGGCTG CAGCACATGC TCACACTCCT GGGAGCTTTC 
AATGTTTGTA ATAAGATCGT GTTCATGCTG AGCTTTGTGG GTAACCGTGG GTCATTCACC 
CGGGGTTATC GGGTGATGGT CATGGATAGA GAGTTCCTGT TTCATCTGCT GTACCTTCTC 
ATCTGCATAC TGGGCCTTTT TGGACACGTC TTCTTTTACA GTCTTCTGTT GTTTGACCTG 
GTGAACCGGG AAGAGACTCT TCTGAACGTC ATCAAGAGTG TGACTCGAAA CGGCCGCTCC 
ATCGTCTTGA CCGCAGTGCT GGGCCTCATC CTGGTCTACC TCTTTTCTAT TGTTGGATAT 
ATGTTTTTCA AAGATGACTT CATCCTGGAG GTGAACCGCA TCTCCAACGG CACTCTGGAG 
GAGGGGGTGA CTTTACCTGC TGGTTTTTGT GATGCGAGAA ACGGGTCGTG TCTTTCTGAT 
GATGTTGACG AGGATGACGT GGAGCGCGCG TGTGATTCTC TATGGATGTG TATGATCACT 
GTACTCAGTC ATGGCCTCAG GAGCGGTGGA GGAGTTGGAG ATGTCCTGCG CAAACCGTCT 
AAAGAGGAGC GTCTGTTCGC CGCACGGGTG GTGTACGATC TCCTGTTCTT CTTCCTGGTG 
ATCATCATTG TGTTGAATCT GATCTTCGGT GTGATCATTG ACACATTTGC AGATCTGAGA 
AGCGAGAAGC AGAGAAAAGA GGAGATCTTG AAGACCACAT GCTTCATCTG TGGTCTGGAG 
AGAGATAAGT TTGATAATAA AACGGTGACG TTTGAAGAGC ACATCAAGGA GGAGCACAAC 
CTGTGGCATT ACCTCTTCTT CATAGTGCTG GTGCGGGTCA AAGACTCCAC CGAATACACG 
GGACCCGAGA GCTACGTCGC TCAGATGATC AAGGAGCACA ATCTGGACTG GTTTCCTCGT 
ATGAGGGCGA TGTCTCTGGT CAGCAGTGAT GGAGAAGGTG AACAGAATGA ACTGCGCAGC 
TTACAGGAGA AACTCGAGTC CACAATGAGG CTCGTCTCTA ATCTTACCAA TCAGCTCACA 
GAGCTCAAAG AGCAGATGAC TGAGCAAAGG AAACACAAGC AGAGGCTCGG TCTGCTGGGA 
AACCCCGCCC ACCTCAACAT CAACCCACAG CAGCCAGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itpr1a ( XM_021474620.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330295.1 Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X16, mRNA.

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CloneID	ODa146879
Clone ID Related Accession (Same CDS sequence)	XM_021474620.1
Accession Version	XM_021474620.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8037bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395382.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGACAT TACAAGGTGC TGGAGTTTTT GCCATGGTGG ACAAATTGTC TAGTTTCCTG 
CACATTGGGG ACGTTTGTTC CCTTTATGCT GAAGGCTCTA CTAGTGGCTT CATCAGCACT 
CTGGGGTTGG TTGATGACCG ATGTGTTGTT CAACCAGATG CCGGAGACCT GAATAACCCA 
CCTAAAAAGT TTCGGGACTG TCTGTTCAGA CTGTGTCCCA TGAACAGATA TTCGGCACAG 
AAACAGTTCT GGAAAGCTGC CAAACCTGGA GGAACTAGCA CCACAGATAC TGTCCTGCTT 
AACAAACTCC ATCATGCTGC TGATTTAGAG AAGAAACAAA ATGATTCTGA GAACAAAAAG 
CTGCTTGGAA CCGTCATTCA GTATGGCAAT GTCATCCAGC TTCTCCATCT GAAGAGTAAT 
AAATATCTGA CGGTGAATAA GCGTTTGCCG GCTCTTCTTG AGAAGAATGC CATGCGTGTG 
ACTCTGGACG CTGCAGGGAA TGAAGGCTCG TGGTTTTACA TCCAGCCCTT CTATAAGCTG 
CGCTCTATAG GGGATAATGT GGTCATTGGA GATAAGGTCG TGTTAAACCC TGTCAATGCC 
GGACAGCCGC TGCACGCCAG CAGCCATCAG CTTGTGGATA ATCCAGGCTG CAATGAAGTG 
AATTCAGTGA ACTGCACCAC CAGTTGGAAA ATCGTTCTCT TCATGAAATG GAGCGACAAC 
CAGGAGATTG TCTTGAAAGG AGGTGACGTG GTGAGGCTGT TTCATGCAGA GCAGGAGAAG 
TTTCTGACAT GTGATGAGCA TCGGAAAAAG CAGTATGTGT TTCTGCGTAC CACCGGACGC 
CAGTCTGCAA CCTCCGCCAC TAGTAGCAAA GCACTCTGGG AGGTAGAGGT GGTTCAGCAT 
GATCCCTGCC GAGGCGGAGC CGGCTACTGG AACAGCCTCT TTAGGTTTAA ACACTTAGCC 
ACCGGCCATT ACCTTGCTGC AGAGATGGAT TCGGAGCATG AAGTGATTCG GGCTCGTGCA 
CGTAACCCTC AGGATAAGGT GATGTACACA CTGGTGTCTG TTCCTGATGG AAATGACATA 
TCCTCCATCT TTGAGCTTGA CCCTACAACT TTGAGGGGCG GAGACTCACT TGTGCCAAGA 
AACTCGTATG TGAGGCTCAG GCATCTCTGC ACCAACACGT GGGTCCACAG CACCAACCAG 
CCAATAGATA AAGAGGAAGA GAAACCGGTC ATGCTCAGAA TAGGAACGTC TCCTCTGAAG 
GAAGATAAAG AGGCGTTTGC TATAGTACCA GTCTCTCCAG CTGAGGTTCG AGATCTGGAT 
TTTGCTAATG ATGCTAGTAA AGTGTTGGCC TCCATTGCTG CCAAACTGGA GAAGGGCACC 
ATCACCCAGA ATGAGAGGAG GTCAGTGACG AAGCTGTTGG AGGATCTGGT GTATTTTGTG 
GTGGACATAC CCAGCAGTGC TCAAGATGTG CTGGAGATCA CAGTCAACAA ACCCAACAGA 
GAGCGACAGA AACTCATGAG AGAGCAGAAC ATTCTTAAAC AGATCTTCAA GCTGCTGCAG 
GCGCCATTTA CAGACAGCGG GGACGGCCCT ATGTTGCGAT TAGAAGAGCT CGCAGACCAG 
CGTCATGCAC CCTTTCGACA CATTTGCAGA CTCTGTTACA GAGTCCTACG TTACTCACAA 
CAGGACTATC GCAAAAATCA GGAGTACATA GCAAAGCAGT TCCGCTTCAT GCAGAAGCAG 
ATAGGATATG ATGTGCTGGC TGAAGACACA ATTACAGCCC TTCTGCACAA CAACAGGAAG 
CTCCTAGAGA AACACATCAC AGCAGCTGAA ATAGATACCT TTGTCAGTCT GGTCCGCAAG 
AACAGAGAGC CCAGATTTCT GGATTACTTG TCAGATCTGT GTGTGTCGAT GAATAAATCC 
ATCCCGGTCA CTCAAGAGCT CATCTGTAAT GCTGTGCTGG ACCCAGCCAA TGCAGACATT 
CTCATCGAAA CCAAGCTTGT GTTGTCACGA TTTGAGGTGG CAGGAACGGT GTTGGGTGAA 
GGTGCGGAGG AGGAAGAGGA GGATGAAGAG GAGGTCTGGC TGTTCTGGAA GGACAGTGGA 
GGAGAGGTGA AGAGTAAAAG CATACGAGAG CTTGCACAGG ACGCAAAAGA TGGACATACA 
GAGGACCAGG AAGTCATCAA TTATTACAGA TATCAGCTGA ATTTGTTTGC TCGGATGTGT 
CTGGATCGAC AGTATCTTGC GATTAACAAG ATATCAGCAC AGCTGGATGT GGATCTGATT 
CTGCGCTGCA TGTCAGATGA AGATTTGCCC TTTGACCTCA GAGCATCGTT CTGCCGCATG 
ATGCTGCACA TGCATGTAGA CCGTGACCCT CAGGAGCAGG TCACCCCGGT CAAATATGCT 
CGACTGTGGT CTGAAATCCC ATCACAAATT TCCATTGATG ATTATGATAA TGATGGGACG 
TCACGTGATG AAGTGAAAGA GCGCTTCTCT CAGACGATGG AGTTTGTGGA GAATTACCTG 
AGGGATGTTG TCTGCCAGAG TTTCCCGTTC TCTGATAAGG AGAAAAACAA ACTCACTTTT 
GAGGTGGTGA ACCTGGCAAG AAACCTGATC TATTTTGGTT TCTATAACTT CAGTGATCTG 
CTAAGGTTAA CAAAAATCCT ATTGGCAATC CTGGACTGTG TCCACATAAG CACTCCCTTC 
CACATCAAGC TGGATAGAGA ACCTGGTAAA GGAAGTAACG TGATGCGCTC CATCCATGGG 
GTGGGAGAAC TGATGACACA GGTAGTGTTG AGGGGTGGGG GATTTCTGCC AGCCTCCAGC 
CACAATCCTC CTGACAGAGA CGAAGTGAAA GCACAAAGTG AACCTCAGAA ACAAGACATA 
CTGGTGATGG ACACTAAACT CAAGATCATC GAGATCCTGC AGTTTATTCT GAATGTGCGG 
TTGGACTACA GGATCTCCTG CCTCCTCTGT ATCTTTAAGA CAGAGTTTGA TGAAAGTAAC 
TCTCAAAGTG AACCATCTTT AAGCCCAGAA TCTCCTGCCA GCGTTCAAGG TGCGCTAGAC 
TTTGAGCACA TTGAAGAGCA GGCGGAGGGT ATTTTTGGAG GAAGTGAAGA GAACACCCCG 
CTGGATCTTG ATGATCATGG TGGACGGACA TTTCTGAGGG TCCTGCTTCA CCTGACCATG 
CATGACTATC CTCCGCTGGT ATCTCGAGCT CTTCACCTGC TCTTCAGACA CTTTAGCCAA 
CGCCAGGAGG TCCTACAGGC CTTCAAACAG GTTCAGCTCC TGGTGACCAG TCAGGATGTG 
GAAAACTATA AACAGATCAA GTCAGATTTG GACCAGCTGA GGTCAATAGT AGAGAAATCA 
GAGTTGTGGG TGTATAAGAG ACAAGGCTCA GACTCAGGAC TGGATGCTGG AGAAGTCACC 
CCTGAAGCCC ACCACAAGGG TGTCATCAAC TCGAGTAGAT CAAACAAACC CAAAGTGGAA 
AGTACCAGCA GCTCGAACTA CAGAGTCGTG AAAGAGATCC TGCTGAGGCT GAGCAAACTG 
TGTGTTTTGG AGGGCATCTC TGGCAAGAAG AATAAGAAAC AGCAGCAGCG GCTCCTCAGG 
AACATGGGAG CTCACAGTGT GGTCCTCGAA CTGTTGCAAA TCCCTTATGA GAAGGGGGAG 
GATGTCCAGA TGCAGGAGAT CATGACCTTA GCACATCAGT TCCTTCAAAA CTTCTGTGCT 
GGAAATCAGC AGAACCAGGC ACTTCTACAC AAACACATCA ACCTGTTCCT CAACCCTGGG 
ATCCTGGAGG CGGTGACCAT GCAGCATATC TTCATGAATA ATTTCCAGTT GTGTAGTGAG 
ATTAATGAGC GGGTCGTGCA GCATTTTGTG CACTGCATCG AGACACACGG CCGCAGCGTG 
CACTACCTCA AGTTCCTGCA GACCATTGTC AAGGCAGAGA ACAAGTTCAT CAAGAAATGC 
CAAGACATCG TCATGGCTGA GCTGGTGACT GCAGGTGAGG ATGTGCTGGT GTTCTATAAT 
GACCGGGCGT CTTTTCAGTC ATTGGTTCAG ATGATGCGTC TTGAGCGGGA ACGACTTGAT 
GAGAGCAGTG CACTCAGGTA TCACATTCAC CTGGTGGAGT TGCTGGCTGT GTGCACTGAA 
GGCAAGAACG TCTACACTGA GATCAAATGC AACTCGCTGC TCCCTCTCGA TGATATTGTA 
CGTGTGGTCA CACATGAGGA CTGCATCCCT GAGGTGAAGA TGGCCTACGT GAACTTCCTG 
AATCACTGTT ATGTTGATAC AGAGGTTGAG ATGAAGGAGA TCTACACCAG CAATCACATG 
TGGAAACTTT TTGATGATTT TCTGGTGGAC GTCTGCAGAG TGTGCAATAA CACAAGTGAC 
CGTAAACACG CTGACACAGT TCTGGAGAGA TACGTTACAG AGACCATAAT GAGCATCGTC 
ACCACCTTTT TCAGCTCACC ATTCTCAGAC CAGAGCACAA GTTTGCAGAC CCGGCAGCCG 
GTGTTTGTGC AGCTGCTGCA GGGAGTGTTT CGTGTATATC ACTGCAACTG GCTTCTCCCA 
GGTCAGAAGG GGAATGTGGA AGCCTGCATC AAAGTGCTGA GTGACGTGGC CAAAGGCAGA 
GCCATAGCCA TTCCTGTGGA TCTGGACTGT CAGGTGAATA ATCTGTTCAT GAAGTCCAAC 
AACATTGTGC AGAAAACAGC CCTCAGCTGG AGACTCTCTG TCCGTAACGC GACTCGGAGA 
GACTCTGTTC TGACCACCTC TCGAGACTAT CGCAACATCA TTGAAAGACT ACAGGATATA 
GTGTCTGCAT TGGAGGAGCG GTTGAGGCCT CTGGTTCAGG CTGAGTTGTC AGTGTTGGTG 
GATGTTCTCC ATCGCCCAGA GCTGCTGTTT CCAGAGCACA CTGAAGCCCA CCGCAAGTGT 
GAGAGTGGAG GCTTCATCTG CAAGTTGATA AAACACACTA AGCAGCTTCT AGAAGAAAAC 
GAAGAGAGGC TTTGCATCAA AGTCCTTCAG ACACTCCGAG AGATGATGAC CAAAGACAGA 
GGATATGGAG AGAAGGGGGA GGCCCTTCGG CAGCTGCTCG TCAATCGTTA CTATGGCTTC 
AGGAGTGGTG GGCGGAGAGA AAGTCTGACC TCGTTTGGTA ACTCCACCCT AACTCCTGTC 
GGGCCAATCA AGAGCCAGCC AGGGGGGTTG AGCAGGGCTG AGATGTCATT AATGGAGGTG 
CAGTGTCATC TAGACCGTGA AGGGGCATCG GATCTTGTCA TTGATCTCAT CATGAACACT 
ACCAGCGACC GCGTCTTCCA TGAAAGCATT CTGTTGGCCA TCGCACTGTT GGAGGGCGGA 
AACACCATCA TACAGCATTC CTTCTTCAAA CGTTTAACAG AAGACAAAAA TTCAGAGAAG 
TTTTTCAGGG TGTTTTATGA CAGAATGAAA GTGGCTCAGG TGGAGATCAA AGCCACGGTT 
ACTGTCAACA CTAGTGACCT CGGCAACAAG CGGCGTGACG ACAACACTCC AGACAAAGAT 
ACTCCACAAC GCCGCAGAGG TAAAGACTCC GGTGTGGTGG TGACAGATGA TGCTCGTGAG 
CAGCTGTTGG AGGCTTCAGC TGCGACTAAG AAGGCCTTTG GTTCATATCG TCGAGATGCT 
GATCCCGAGG AGACGTTTGG CACGGCTGAT GGAGATAAAG GAGGTGGAGA TAAAGGCACA 
GAGCAGGGCG AGATGAGCCC TGTCATCCTC ATAATGCAGC CAATCCTGCG CTTCCTGCAG 
CTGCTGTGCG AAAACCACAA TCGAGACCTG CAGAATTTTC TGCGCTGTCA GAACAATAAA 
AACAACTATA ATCTGGTGTG TGAGACGCTG CAGTTCCTGG ACTGTATCTG TGGCAGCACA 
ACAGGAGGCC TGGGGCTGTT AGGACTCTAC ATCAACCAGC ATAACGTAGC GCTCATTAAT 
CAGACTGTAG AGAGCCTCAC TGAATACTGT CAAGGTCCCT GCCATGACAA TCAGAATTGC 
ATTGCCACCC ATGAGTCTAA TGGCATCGAC ATCATTATTG CACTGATTCT GAATGATATT 
AATCCACTGG GCAGGAAGAG AATGGATCTG GTTCTAGAAC TAAAGAATAA TGCATCTAAG 
CTGCTGCTGG CCATCATGGA GAGCCGTCAT GACAGTGAGA ATGCAGAAAG AATTCTGTAC 
AACATGAGAC CAAAAGAACT AGTGGAGGTG ATGAAAATGG CCTACCAGCA GGGGGAGGCA 
GAGTTTGAGG ATGAGGAACA GGAGAATGGA GAGGATCATG CAGCATCGCC TCGCAATGTT 
GGACACAACA TCTATATTCT TGCACATCAG CTGTCGCGTC ACAATAAGGA ATTGCAAATA 
CTACTGAAAC CAGGTGGAGA GGACCAGGCC CTGGAGTATT ACACAAAACA CACCTCACAG 
ATAGAGATAG TTCGACAGGA TCGCACTATG GAACAGATTG TGTTTCCTGT ACCAAATATC 
TGCTCTTTTC TGACAAATGA GTCCAAGCTG CGTGTGTACT ATGGTACAGA GAGAGACGAG 
CAAGGCAGTA AAATAAATGA CTTCTTCTTG CATGCAGACG ACCTCTTTAA TGAAATGAGG 
TGGCAGAAAA AACTAAGAGC TCAGCCAGTG CTGTACTGGT GCAGCAGGAA CATGTCGTTT 
TGGAGTAACG TTTCCTTTAA TCTGGCGGTG CTCATTAATT TGCTGGTGGC ATTTTTCTAC 
CCTCTGGATG GAGTGAGCGA AAGCCAGCTG GAACCTTCTC TGTCTCTGTT GCTGTGGGTT 
TGTTTGTTGG GCTCACTGGG CTTCGTGTTG ATGTCCCCGA GGCCAAATGC AGTGCGGGTG 
CTGGTCATCT CCACTGTCCT GCAGTTGGGC TTCTCTGTAG GGCTGCAGCA CATGCTCACA 
CTCCTGGGAG CTTTCAATGT TTGTAATAAG ATCGTGTTCA TGCTGAGCTT TGTGGGTAAC 
CGTGGGTCAT TCACCCGGGG TTATCGGGTG ATGGTCATGG ATAGAGAGTT CCTGTTTCAT 
CTGCTGTACC TTCTCATCTG CATACTGGGC CTTTTTGGAC ACGTCTTCTT TTACAGTCTT 
CTGTTGTTTG ACCTGGTGAA CCGGGAAGAG ACTCTTCTGA ACGTCATCAA GAGTGTGACT 
CGAAACGGCC GCTCCATCGT CTTGACCGCA GTGCTGGGCC TCATCCTGGT CTACCTCTTT 
TCTATTGTTG GATATATGTT TTTCAAAGAT GACTTCATCC TGGAGGTGAA CCGCATCTCC 
AACGGCACTC TGGAGGAGGG GGTGACTTTA CCTGCTGGTT TTTGTGATGC GAGAAACGGG 
TCGTGTCTTT CTGATGATGT TGACGAGGAT GACGTGGAGC GCGCGTGTGA TTCTCTATGG 
ATGTGTATGA TCACTGTACT CAGTCATGGC CTCAGGAGCG GTGGAGGAGT TGGAGATGTC 
CTGCGCAAAC CGTCTAAAGA GGAGCGTCTG TTCGCCGCAC GGGTGGTGTA CGATCTCCTG 
TTCTTCTTCC TGGTGATCAT CATTGTGTTG AATCTGATCT TCGGTGTGAT CATTGACACA 
TTTGCAGATC TGAGAAGCGA GAAGCAGAGA AAAGAGGAGA TCTTGAAGAC CACATGCTTC 
ATCTGTGGTC TGGAGAGAGA TAAGTTTGAT AATAAAACGG TGACGTTTGA AGAGCACATC 
AAGGAGGAGC ACAACCTGTG GCATTACCTC TTCTTCATAG TGCTGGTGCG GGTCAAAGAC 
TCCACCGAAT ACACGGGACC CGAGAGCTAC GTCGCTCAGA TGATCAAGGA GCACAATCTG 
GACTGGTTTC CTCGTATGAG GGCGATGTCT CTGGTCAGCA GTGATGGAGA AGGTGAACAG 
AATGAACTGC GCAGCTTACA GGAGAAACTC GAGTCCACAA TGAGGCTCGT CTCTAATCTT 
ACCAATCAGC TCACAGAGCT CAAAGAGCAG ATGACTGAGC AAAGGAAACA CAAGCAGAGG 
CTCGGTCTGC TGGGAAACCC CGCCCACCTC AACATCAACC CACAGCAGCC AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330295.1
CDS	265..8301
Translation	MMTLQGAGVFAMVDKLSSFLHIGDVCSLYAEGSTSGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGTSTTDTVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDAAGNEGSWFYIQPFYKLRSIGDNVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCTTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDEHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEMDSEHEVIRARARNPQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSPLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAAKLEKGTITQNERRSVTKLLEDLVYFVVDIPSSAQDVLEITVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRYSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDPANADILIETKLVLSRFEVAGTVLGEGAEEEEEDEEEVWLFWKDSGGEVKSKSIRELAQDAKDGHTEDQEVINYYRYQLNLFARMCLDRQYLAINKISAQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSQISIDDYDNDGTSRDEVKERFSQTMEFVENYLRDVVCQSFPFSDKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHISTPFHIKLDREPGKGSNVMRSIHGVGELMTQVVLRGGGFLPASSHNPPDRDEVKAQSEPQKQDILVMDTKLKIIEILQFILNVRLDYRISCLLCIFKTEFDESNSQSEPSLSPESPASVQGALDFEHIEEQAEGIFGGSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALHLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSDSGLDAGEVTPEAHHKGVINSSRSNKPKVESTSSSNYRVVKEILLRLSKLCVLEGISGKKNKKQQQRLLRNMGAHSVVLELLQIPYEKGEDVQMQEIMTLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAVTMQHIFMNNFQLCSEINERVVQHFVHCIETHGRSVHYLKFLQTIVKAENKFIKKCQDIVMAELVTAGEDVLVFYNDRASFQSLVQMMRLERERLDESSALRYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKMAYVNFLNHCYVDTEVEMKEIYTSNHMWKLFDDFLVDVCRVCNNTSDRKHADTVLERYVTETIMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQGVFRVYHCNWLLPGQKGNVEACIKVLSDVAKGRAIAIPVDLDCQVNNLFMKSNNIVQKTALSWRLSVRNATRRDSVLTTSRDYRNIIERLQDIVSALEERLRPLVQAELSVLVDVLHRPELLFPEHTEAHRKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKGEALRQLLVNRYYGFRSGGRRESLTSFGNSTLTPVGPIKSQPGGLSRAEMSLMEVQCHLDREGASDLVIDLIMNTTSDRVFHESILLAIALLEGGNTIIQHSFFKRLTEDKNSEKFFRVFYDRMKVAQVEIKATVTVNTSDLGNKRRDDNTPDKDTPQRRRGKDSGVVVTDDAREQLLEASAATKKAFGSYRRDADPEETFGTADGDKGGGDKGTEQGEMSPVILIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINQHNVALINQTVESLTEYCQGPCHDNQNCIATHESNGIDIIIALILNDINPLGRKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVMKMAYQQGEAEFEDEEQENGEDHAASPRNVGHNIYILAHQLSRHNKELQILLKPGGEDQALEYYTKHTSQIEIVRQDRTMEQIVFPVPNICSFLTNESKLRVYYGTERDEQGSKINDFFLHADDLFNEMRWQKKLRAQPVLYWCSRNMSFWSNVSFNLAVLINLLVAFFYPLDGVSESQLEPSLSLLLWVCLLGSLGFVLMSPRPNAVRVLVISTVLQLGFSVGLQHMLTLLGAFNVCNKIVFMLSFVGNRGSFTRGYRVMVMDREFLFHLLYLLICILGLFGHVFFYSLLLFDLVNREETLLNVIKSVTRNGRSIVLTAVLGLILVYLFSIVGYMFFKDDFILEVNRISNGTLEEGVTLPAGFCDARNGSCLSDDVDEDDVERACDSLWMCMITVLSHGLRSGGGVGDVLRKPSKEERLFAARVVYDLLFFFLVIIIVLNLIFGVIIDTFADLRSEKQRKEEILKTTCFICGLERDKFDNKTVTFEEHIKEEHNLWHYLFFIVLVRVKDSTEYTGPESYVAQMIKEHNLDWFPRMRAMSLVSSDGEGEQNELRSLQEKLESTMRLVSNLTNQLTELKEQMTEQRKHKQRLGLLGNPAHLNINPQQPA

Target ORF information:

RefSeq Version	XM_021474620.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474620.1

ORF Insert Sequence:

ATGATGACAT TACAAGGTGC TGGAGTTTTT GCCATGGTGG ACAAATTGTC TAGTTTCCTG 
CACATTGGGG ACGTTTGTTC CCTTTATGCT GAAGGCTCTA CTAGTGGCTT CATCAGCACT 
CTGGGGTTGG TTGATGACCG ATGTGTTGTT CAACCAGATG CCGGAGACCT GAATAACCCA 
CCTAAAAAGT TTCGGGACTG TCTGTTCAGA CTGTGTCCCA TGAACAGATA TTCGGCACAG 
AAACAGTTCT GGAAAGCTGC CAAACCTGGA GGAACTAGCA CCACAGATAC TGTCCTGCTT 
AACAAACTCC ATCATGCTGC TGATTTAGAG AAGAAACAAA ATGATTCTGA GAACAAAAAG 
CTGCTTGGAA CCGTCATTCA GTATGGCAAT GTCATCCAGC TTCTCCATCT GAAGAGTAAT 
AAATATCTGA CGGTGAATAA GCGTTTGCCG GCTCTTCTTG AGAAGAATGC CATGCGTGTG 
ACTCTGGACG CTGCAGGGAA TGAAGGCTCG TGGTTTTACA TCCAGCCCTT CTATAAGCTG 
CGCTCTATAG GGGATAATGT GGTCATTGGA GATAAGGTCG TGTTAAACCC TGTCAATGCC 
GGACAGCCGC TGCACGCCAG CAGCCATCAG CTTGTGGATA ATCCAGGCTG CAATGAAGTG 
AATTCAGTGA ACTGCACCAC CAGTTGGAAA ATCGTTCTCT TCATGAAATG GAGCGACAAC 
CAGGAGATTG TCTTGAAAGG AGGTGACGTG GTGAGGCTGT TTCATGCAGA GCAGGAGAAG 
TTTCTGACAT GTGATGAGCA TCGGAAAAAG CAGTATGTGT TTCTGCGTAC CACCGGACGC 
CAGTCTGCAA CCTCCGCCAC TAGTAGCAAA GCACTCTGGG AGGTAGAGGT GGTTCAGCAT 
GATCCCTGCC GAGGCGGAGC CGGCTACTGG AACAGCCTCT TTAGGTTTAA ACACTTAGCC 
ACCGGCCATT ACCTTGCTGC AGAGATGGAT TCGGAGCATG AAGTGATTCG GGCTCGTGCA 
CGTAACCCTC AGGATAAGGT GATGTACACA CTGGTGTCTG TTCCTGATGG AAATGACATA 
TCCTCCATCT TTGAGCTTGA CCCTACAACT TTGAGGGGCG GAGACTCACT TGTGCCAAGA 
AACTCGTATG TGAGGCTCAG GCATCTCTGC ACCAACACGT GGGTCCACAG CACCAACCAG 
CCAATAGATA AAGAGGAAGA GAAACCGGTC ATGCTCAGAA TAGGAACGTC TCCTCTGAAG 
GAAGATAAAG AGGCGTTTGC TATAGTACCA GTCTCTCCAG CTGAGGTTCG AGATCTGGAT 
TTTGCTAATG ATGCTAGTAA AGTGTTGGCC TCCATTGCTG CCAAACTGGA GAAGGGCACC 
ATCACCCAGA ATGAGAGGAG GTCAGTGACG AAGCTGTTGG AGGATCTGGT GTATTTTGTG 
GTGGACATAC CCAGCAGTGC TCAAGATGTG CTGGAGATCA CAGTCAACAA ACCCAACAGA 
GAGCGACAGA AACTCATGAG AGAGCAGAAC ATTCTTAAAC AGATCTTCAA GCTGCTGCAG 
GCGCCATTTA CAGACAGCGG GGACGGCCCT ATGTTGCGAT TAGAAGAGCT CGCAGACCAG 
CGTCATGCAC CCTTTCGACA CATTTGCAGA CTCTGTTACA GAGTCCTACG TTACTCACAA 
CAGGACTATC GCAAAAATCA GGAGTACATA GCAAAGCAGT TCCGCTTCAT GCAGAAGCAG 
ATAGGATATG ATGTGCTGGC TGAAGACACA ATTACAGCCC TTCTGCACAA CAACAGGAAG 
CTCCTAGAGA AACACATCAC AGCAGCTGAA ATAGATACCT TTGTCAGTCT GGTCCGCAAG 
AACAGAGAGC CCAGATTTCT GGATTACTTG TCAGATCTGT GTGTGTCGAT GAATAAATCC 
ATCCCGGTCA CTCAAGAGCT CATCTGTAAT GCTGTGCTGG ACCCAGCCAA TGCAGACATT 
CTCATCGAAA CCAAGCTTGT GTTGTCACGA TTTGAGGTGG CAGGAACGGT GTTGGGTGAA 
GGTGCGGAGG AGGAAGAGGA GGATGAAGAG GAGGTCTGGC TGTTCTGGAA GGACAGTGGA 
GGAGAGGTGA AGAGTAAAAG CATACGAGAG CTTGCACAGG ACGCAAAAGA TGGACATACA 
GAGGACCAGG AAGTCATCAA TTATTACAGA TATCAGCTGA ATTTGTTTGC TCGGATGTGT 
CTGGATCGAC AGTATCTTGC GATTAACAAG ATATCAGCAC AGCTGGATGT GGATCTGATT 
CTGCGCTGCA TGTCAGATGA AGATTTGCCC TTTGACCTCA GAGCATCGTT CTGCCGCATG 
ATGCTGCACA TGCATGTAGA CCGTGACCCT CAGGAGCAGG TCACCCCGGT CAAATATGCT 
CGACTGTGGT CTGAAATCCC ATCACAAATT TCCATTGATG ATTATGATAA TGATGGGACG 
TCACGTGATG AAGTGAAAGA GCGCTTCTCT CAGACGATGG AGTTTGTGGA GAATTACCTG 
AGGGATGTTG TCTGCCAGAG TTTCCCGTTC TCTGATAAGG AGAAAAACAA ACTCACTTTT 
GAGGTGGTGA ACCTGGCAAG AAACCTGATC TATTTTGGTT TCTATAACTT CAGTGATCTG 
CTAAGGTTAA CAAAAATCCT ATTGGCAATC CTGGACTGTG TCCACATAAG CACTCCCTTC 
CACATCAAGC TGGATAGAGA ACCTGGTAAA GGAAGTAACG TGATGCGCTC CATCCATGGG 
GTGGGAGAAC TGATGACACA GGTAGTGTTG AGGGGTGGGG GATTTCTGCC AGCCTCCAGC 
CACAATCCTC CTGACAGAGA CGAAGTGAAA GCACAAAGTG AACCTCAGAA ACAAGACATA 
CTGGTGATGG ACACTAAACT CAAGATCATC GAGATCCTGC AGTTTATTCT GAATGTGCGG 
TTGGACTACA GGATCTCCTG CCTCCTCTGT ATCTTTAAGA CAGAGTTTGA TGAAAGTAAC 
TCTCAAAGTG AACCATCTTT AAGCCCAGAA TCTCCTGCCA GCGTTCAAGG TGCGCTAGAC 
TTTGAGCACA TTGAAGAGCA GGCGGAGGGT ATTTTTGGAG GAAGTGAAGA GAACACCCCG 
CTGGATCTTG ATGATCATGG TGGACGGACA TTTCTGAGGG TCCTGCTTCA CCTGACCATG 
CATGACTATC CTCCGCTGGT ATCTCGAGCT CTTCACCTGC TCTTCAGACA CTTTAGCCAA 
CGCCAGGAGG TCCTACAGGC CTTCAAACAG GTTCAGCTCC TGGTGACCAG TCAGGATGTG 
GAAAACTATA AACAGATCAA GTCAGATTTG GACCAGCTGA GGTCAATAGT AGAGAAATCA 
GAGTTGTGGG TGTATAAGAG ACAAGGCTCA GACTCAGGAC TGGATGCTGG AGAAGTCACC 
CCTGAAGCCC ACCACAAGGG TGTCATCAAC TCGAGTAGAT CAAACAAACC CAAAGTGGAA 
AGTACCAGCA GCTCGAACTA CAGAGTCGTG AAAGAGATCC TGCTGAGGCT GAGCAAACTG 
TGTGTTTTGG AGGGCATCTC TGGCAAGAAG AATAAGAAAC AGCAGCAGCG GCTCCTCAGG 
AACATGGGAG CTCACAGTGT GGTCCTCGAA CTGTTGCAAA TCCCTTATGA GAAGGGGGAG 
GATGTCCAGA TGCAGGAGAT CATGACCTTA GCACATCAGT TCCTTCAAAA CTTCTGTGCT 
GGAAATCAGC AGAACCAGGC ACTTCTACAC AAACACATCA ACCTGTTCCT CAACCCTGGG 
ATCCTGGAGG CGGTGACCAT GCAGCATATC TTCATGAATA ATTTCCAGTT GTGTAGTGAG 
ATTAATGAGC GGGTCGTGCA GCATTTTGTG CACTGCATCG AGACACACGG CCGCAGCGTG 
CACTACCTCA AGTTCCTGCA GACCATTGTC AAGGCAGAGA ACAAGTTCAT CAAGAAATGC 
CAAGACATCG TCATGGCTGA GCTGGTGACT GCAGGTGAGG ATGTGCTGGT GTTCTATAAT 
GACCGGGCGT CTTTTCAGTC ATTGGTTCAG ATGATGCGTC TTGAGCGGGA ACGACTTGAT 
GAGAGCAGTG CACTCAGGTA TCACATTCAC CTGGTGGAGT TGCTGGCTGT GTGCACTGAA 
GGCAAGAACG TCTACACTGA GATCAAATGC AACTCGCTGC TCCCTCTCGA TGATATTGTA 
CGTGTGGTCA CACATGAGGA CTGCATCCCT GAGGTGAAGA TGGCCTACGT GAACTTCCTG 
AATCACTGTT ATGTTGATAC AGAGGTTGAG ATGAAGGAGA TCTACACCAG CAATCACATG 
TGGAAACTTT TTGATGATTT TCTGGTGGAC GTCTGCAGAG TGTGCAATAA CACAAGTGAC 
CGTAAACACG CTGACACAGT TCTGGAGAGA TACGTTACAG AGACCATAAT GAGCATCGTC 
ACCACCTTTT TCAGCTCACC ATTCTCAGAC CAGAGCACAA GTTTGCAGAC CCGGCAGCCG 
GTGTTTGTGC AGCTGCTGCA GGGAGTGTTT CGTGTATATC ACTGCAACTG GCTTCTCCCA 
GGTCAGAAGG GGAATGTGGA AGCCTGCATC AAAGTGCTGA GTGACGTGGC CAAAGGCAGA 
GCCATAGCCA TTCCTGTGGA TCTGGACTGT CAGGTGAATA ATCTGTTCAT GAAGTCCAAC 
AACATTGTGC AGAAAACAGC CCTCAGCTGG AGACTCTCTG TCCGTAACGC GACTCGGAGA 
GACTCTGTTC TGACCACCTC TCGAGACTAT CGCAACATCA TTGAAAGACT ACAGGATATA 
GTGTCTGCAT TGGAGGAGCG GTTGAGGCCT CTGGTTCAGG CTGAGTTGTC AGTGTTGGTG 
GATGTTCTCC ATCGCCCAGA GCTGCTGTTT CCAGAGCACA CTGAAGCCCA CCGCAAGTGT 
GAGAGTGGAG GCTTCATCTG CAAGTTGATA AAACACACTA AGCAGCTTCT AGAAGAAAAC 
GAAGAGAGGC TTTGCATCAA AGTCCTTCAG ACACTCCGAG AGATGATGAC CAAAGACAGA 
GGATATGGAG AGAAGGGGGA GGCCCTTCGG CAGCTGCTCG TCAATCGTTA CTATGGCTTC 
AGGAGTGGTG GGCGGAGAGA AAGTCTGACC TCGTTTGGTA ACTCCACCCT AACTCCTGTC 
GGGCCAATCA AGAGCCAGCC AGGGGGGTTG AGCAGGGCTG AGATGTCATT AATGGAGGTG 
CAGTGTCATC TAGACCGTGA AGGGGCATCG GATCTTGTCA TTGATCTCAT CATGAACACT 
ACCAGCGACC GCGTCTTCCA TGAAAGCATT CTGTTGGCCA TCGCACTGTT GGAGGGCGGA 
AACACCATCA TACAGCATTC CTTCTTCAAA CGTTTAACAG AAGACAAAAA TTCAGAGAAG 
TTTTTCAGGG TGTTTTATGA CAGAATGAAA GTGGCTCAGG TGGAGATCAA AGCCACGGTT 
ACTGTCAACA CTAGTGACCT CGGCAACAAG CGGCGTGACG ACAACACTCC AGACAAAGAT 
ACTCCACAAC GCCGCAGAGG TAAAGACTCC GGTGTGGTGG TGACAGATGA TGCTCGTGAG 
CAGCTGTTGG AGGCTTCAGC TGCGACTAAG AAGGCCTTTG GTTCATATCG TCGAGATGCT 
GATCCCGAGG AGACGTTTGG CACGGCTGAT GGAGATAAAG GAGGTGGAGA TAAAGGCACA 
GAGCAGGGCG AGATGAGCCC TGTCATCCTC ATAATGCAGC CAATCCTGCG CTTCCTGCAG 
CTGCTGTGCG AAAACCACAA TCGAGACCTG CAGAATTTTC TGCGCTGTCA GAACAATAAA 
AACAACTATA ATCTGGTGTG TGAGACGCTG CAGTTCCTGG ACTGTATCTG TGGCAGCACA 
ACAGGAGGCC TGGGGCTGTT AGGACTCTAC ATCAACCAGC ATAACGTAGC GCTCATTAAT 
CAGACTGTAG AGAGCCTCAC TGAATACTGT CAAGGTCCCT GCCATGACAA TCAGAATTGC 
ATTGCCACCC ATGAGTCTAA TGGCATCGAC ATCATTATTG CACTGATTCT GAATGATATT 
AATCCACTGG GCAGGAAGAG AATGGATCTG GTTCTAGAAC TAAAGAATAA TGCATCTAAG 
CTGCTGCTGG CCATCATGGA GAGCCGTCAT GACAGTGAGA ATGCAGAAAG AATTCTGTAC 
AACATGAGAC CAAAAGAACT AGTGGAGGTG ATGAAAATGG CCTACCAGCA GGGGGAGGCA 
GAGTTTGAGG ATGAGGAACA GGAGAATGGA GAGGATCATG CAGCATCGCC TCGCAATGTT 
GGACACAACA TCTATATTCT TGCACATCAG CTGTCGCGTC ACAATAAGGA ATTGCAAATA 
CTACTGAAAC CAGGTGGAGA GGACCAGGCC CTGGAGTATT ACACAAAACA CACCTCACAG 
ATAGAGATAG TTCGACAGGA TCGCACTATG GAACAGATTG TGTTTCCTGT ACCAAATATC 
TGCTCTTTTC TGACAAATGA GTCCAAGCTG CGTGTGTACT ATGGTACAGA GAGAGACGAG 
CAAGGCAGTA AAATAAATGA CTTCTTCTTG CATGCAGACG ACCTCTTTAA TGAAATGAGG 
TGGCAGAAAA AACTAAGAGC TCAGCCAGTG CTGTACTGGT GCAGCAGGAA CATGTCGTTT 
TGGAGTAACG TTTCCTTTAA TCTGGCGGTG CTCATTAATT TGCTGGTGGC ATTTTTCTAC 
CCTCTGGATG GAGTGAGCGA AAGCCAGCTG GAACCTTCTC TGTCTCTGTT GCTGTGGGTT 
TGTTTGTTGG GCTCACTGGG CTTCGTGTTG ATGTCCCCGA GGCCAAATGC AGTGCGGGTG 
CTGGTCATCT CCACTGTCCT GCAGTTGGGC TTCTCTGTAG GGCTGCAGCA CATGCTCACA 
CTCCTGGGAG CTTTCAATGT TTGTAATAAG ATCGTGTTCA TGCTGAGCTT TGTGGGTAAC 
CGTGGGTCAT TCACCCGGGG TTATCGGGTG ATGGTCATGG ATAGAGAGTT CCTGTTTCAT 
CTGCTGTACC TTCTCATCTG CATACTGGGC CTTTTTGGAC ACGTCTTCTT TTACAGTCTT 
CTGTTGTTTG ACCTGGTGAA CCGGGAAGAG ACTCTTCTGA ACGTCATCAA GAGTGTGACT 
CGAAACGGCC GCTCCATCGT CTTGACCGCA GTGCTGGGCC TCATCCTGGT CTACCTCTTT 
TCTATTGTTG GATATATGTT TTTCAAAGAT GACTTCATCC TGGAGGTGAA CCGCATCTCC 
AACGGCACTC TGGAGGAGGG GGTGACTTTA CCTGCTGGTT TTTGTGATGC GAGAAACGGG 
TCGTGTCTTT CTGATGATGT TGACGAGGAT GACGTGGAGC GCGCGTGTGA TTCTCTATGG 
ATGTGTATGA TCACTGTACT CAGTCATGGC CTCAGGAGCG GTGGAGGAGT TGGAGATGTC 
CTGCGCAAAC CGTCTAAAGA GGAGCGTCTG TTCGCCGCAC GGGTGGTGTA CGATCTCCTG 
TTCTTCTTCC TGGTGATCAT CATTGTGTTG AATCTGATCT TCGGTGTGAT CATTGACACA 
TTTGCAGATC TGAGAAGCGA GAAGCAGAGA AAAGAGGAGA TCTTGAAGAC CACATGCTTC 
ATCTGTGGTC TGGAGAGAGA TAAGTTTGAT AATAAAACGG TGACGTTTGA AGAGCACATC 
AAGGAGGAGC ACAACCTGTG GCATTACCTC TTCTTCATAG TGCTGGTGCG GGTCAAAGAC 
TCCACCGAAT ACACGGGACC CGAGAGCTAC GTCGCTCAGA TGATCAAGGA GCACAATCTG 
GACTGGTTTC CTCGTATGAG GGCGATGTCT CTGGTCAGCA GTGATGGAGA AGGTGAACAG 
AATGAACTGC GCAGCTTACA GGAGAAACTC GAGTCCACAA TGAGGCTCGT CTCTAATCTT 
ACCAATCAGC TCACAGAGCT CAAAGAGCAG ATGACTGAGC AAAGGAAACA CAAGCAGAGG 
CTCGGTCTGC TGGGAAACCC CGCCCACCTC AACATCAACC CACAGCAGCC AGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itpr1a ( XM_009302585.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300860.1 Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X2, mRNA.

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CloneID	ODa45192
Clone ID Related Accession (Same CDS sequence)	XM_009302585.2 , XM_009302585.3
Accession Version	XM_009302585.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302585.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GGTAAATCCA 
GACTATGAAG AAGAATGCCT GGAGTCCCGC TCCTCTATGG ATTCGGAGCA TGAAGTGATT 
CGGGCTCGTG CACGTAACCC TCAGGATAAG GTGATGTACA CACTGGTGTC TGTTCCTGAT 
GGAAATGACA TATCCTCCAT CTTTGAGCTT GACCCTACAA CTTTGAGGGG CGGAGACTCA 
CTTGTGCCAA GAAACTCGTA TGTGAGGCTC AGGCATCTCT GCACCAACAC GTGGGTCCAC 
AGCACCAACC AGCCAATAGA TAAAGAGGAA GAGAAACCGG TCATGCTCAG AATAGGAACG 
TCTCCTCTGA AGGAAGATAA AGAGGCGTTT GCTATAGTAC CAGTCTCTCC AGCTGAGGTT 
CGAGATCTGG ATTTTGCTAA TGATGCTAGT AAAGTGTTGG CTTCCATTGC TGCCAAACTG 
GAGAAGGGCA CCATTACCCA GAATGAGAGG AGGTCAGTGA CGAAGCTGTT GGAGGATCTG 
GTGTATTTTG TGGTGGACAT ACCCAGCAGT GCTCAAGATG TGCTGGAGAT CACAGTCAAC 
AAACCCAACA GAGAGCGACA GAAACTCATG AGAGAGCAGA ACATTCTTAA ACAGATCTTC 
AAGCTGCTGC AGGCGCCATT TACAGACAGC GGGGACGGCC CTATGTTGCG ATTAGAAGAG 
CTCGCAGACC AGCGTCATGC ACCCTTTCGA CACATTTGCA GACTCTGTTA CAGAGTCCTA 
CGTTACTCAC AACAGGACTA TCGCAAAAAT CAGGAGTACA TAGCAAAGCA GTTCCGCTTC 
ATGCAGAAGC AGATAGGATA TGATGTGCTG GCTGAAGACA CAATTACAGC CCTTCTGCAC 
AACAACAGGA AGCTCCTAGA GAAACACATC ACAGCAGCTG AAATAGATAC CTTTGTCAGT 
CTGGTCCGCA AGAACAGAGA GCCCAGATTT CTGGATTACT TGTCAGATCT GTGTGTGTCG 
ATGAATAAAT CCATCCCGGT CACTCAAGAG CTCATCTGTA ATGCTGTGCT GGACCCAGCC 
AATGCAGACA TTCTCATCGA AACCAAGCTT GTGTTGTCAC GGTTTGAGGT GGCAGGAACG 
GTGTTGGGTG AAGGTGCGGA GGAGGAAGAG GAGGATGAAG AGGAGGTCTG GCTGTTCTGG 
AAGGACAGTG GAGGAGAGGT GAAGAGTAAA AGCATACGAG AGCTTGCACA GGACGCAAAA 
GATGGACATA CAGAGGACCA GGAAGTCATC AATTATTACA GATATCAGCT GAATTTGTTT 
GCTCGGATGT GTCTGGATCG ACAGTATCTT GCGATTAACA AGATATCAGC ACAGCTGGAT 
GTGGATCTGA TTCTGCGCTG CATGTCAGAT GAAGATTTGC CCTTTGACCT CAGAGCATCG 
TTCTGCCGCA TGATGCTGCA CATGCATGTA GACCGTGACC CTCAGGAGCA GGTCACCCCG 
GTCAAATATG CTCGACTGTG GTCTGAAATC CCATCACAAA TTTCCATTGA TGATTATGAT 
AATGATGGGA CGTCAAGTGA TGAAGTGAAA GAGCGCTTCT CTCAGACGAT GGAGTTTGTG 
GAGAATTACC TGAGGGATGT TGTCTGCCAG AGTTTCCCGT TCTCTGATAA GGAGAAAAAC 
AAACTCACTT TTGAGGTGGT GAACCTGGCA AGAAACCTGA TCTATTTTGG TTTCTATAAC 
TTCAGTGATC TGCTAAGGTT AACAAAAATC CTATTGGCAA TCCTGGACTG TGTCCACATA 
AGCACTCCCT TCCACATCAA GCTGGATAGA GAACCCGGTA AAGGAAGTAA CGTGATGCGC 
TCCATCCATG GGGTGGGAGA GCTGATGACA CAGGTGGTGT TGAGGGGTGG GGGATTTCTG 
CCAGCCTCCA GCCACAATCC TCCTGACAGA GACGAAGTGA AAGCACAAAG TGAACCTCAG 
AAACAGGACA TACTGGTGAT GGACACTAAA CTCAAGATCA TCGAGATCCT GCAGTTTATT 
CTGAATGTGC GGTTGGACTA CAGGATCTCC TGCCTCCTCT GTATCTTTAA GACAGAGTTT 
GATGAAAGTA ACTCTCAAAG TGAACCATCT TTAAGCCCAG AATCTCCTGC CAGCGTTCAA 
GGTGCGCTAG ACTTTGAGCA CATTGAAGAG CAGGCGGAGG GTATTTTTGG AGGAAGTGAA 
GAGAACACCC CGCTGGATCT TGATGATCAT GGTGGACGGA CATTTCTGAG GGTCCTGCTT 
CACCTGACCA TGCATGACTA TCCTCCGCTG GTATCTCGAG CTCTTCACCT GCTCTTCAGA 
CACTTTAGCC AACGCCAGGA GGTCCTACAG GCCTTCAAAC AGGTTCAGCT CCTGGTGACC 
AGTCAGGATG TGGAAAACTA TAAACAGATC AAGTCAGATT TGGACCAGCT GAGGTCAATA 
GTAGAGAAAT CAGAGTTGTG GGTGTATAAG AGACAAGGCT CAGACTCAGG ACTGGATGCT 
GGAGAAGTCA CCCCTGAAGC CCACCACAAG GGTGTCATCA ACTCGAGTAG ATCAAACAAA 
CCCAAAGTGG AAAGTACCAG CAGCTCGAAC TACAGAGTCG TGAAAGAGAT CCTGCTGAGG 
CTGAGCAAAC TGTGTGTTTT GGAGGGCATC TCTGGCAAGA AGAATAAGAA ACAGCAGCAG 
CGGCTCCTCA GGAACATGGG AGCTCACAGT GTGGTCCTCG AACTGTTGCA AATCCCTTAT 
GAGAAGGGGG AGGATGTCCA GATGCAGGAG ATCATGACCT TAGCACATCA GTTCCTTCAA 
AACTTCTGTG CTGGAAATCA GCAGAACCAG GCACTTCTAC ACAAACACAT CAACCTGTTC 
CTCAACCCTG GGATCCTGGA GGCGGTGACC ATGCAGCATA TCTTCATGAA TAATTTCCAG 
TTGTGTAGTG AGATTAATGA GCGGGTCGTG CAGCATTTTG TGCACTGCAT CGAGACACAC 
GGCCGCAGCG TGCACTACCT CAAGTTCCTG CAGACCATTG TCAAGGCAGA GAACAAGTTC 
ATCAAGAAAT GCCAAGACAT CGTCATGGCT GAGCTGGTGA CTGCAGGTGA GGATGTGCTG 
GTGTTCTATA ATGACCGGGC GTCTTTTCAG TCATTGGTTC AGATGATGCG TCTTGAGCGG 
GAACGACTTG ATGAGAGCAG TGCACTCAGG TATCACATTC ACCTGGTGGA GTTGCTGGCT 
GTGTGCACTG AAGGCAAGAA CGTCTACACT GAGATCAAAT GCAACTCGCT GCTCCCTCTC 
GATGATATTG TACGTGTGGT CACACATGAG GACTGCATCC CTGAGGTGAA GATGGCCTAC 
GTGAACTTCC TGAATCACTG TTATGTTGAT ACAGAGGTTG AGATGAAGGA GATCTACACC 
AGCAATCACA TGTGGAAACT TTTTGATGAT TTTCTGGTGG ACGTCTGCAG AGTGTGCAAT 
AACACAAGTG ACCGTAAACA CGCTGACACA GTTCTGGAGA GATACGTTAC AGAGACCATA 
ATGAGCATCG TCACCACCTT TTTCAGCTCA CCATTCTCAG ACCAGAGCAC AAGTTTGCAG 
ACCCGGCAGC CGGTGTTTGT GCAGCTGCTG CAGGGAGTGT TTCGTGTATA TCACTGCAAC 
TGGCTTCTCC CAGGTCAGAA GGGGAATGTG GAAGCCTGCA TCAAAGTGCT GAGTGACGTG 
GCCAAAGGCA GAGCCATAGC CATTCCTGTG GATCTGGACT GTCAGGTGAA TAATCTGTTC 
ATGAAGTCCA ACAACATTGT GCAGAAAACA GCCCTCAGCT GGAGACTCTC TGTCCGTAAC 
GCGACTCGGA GAGACTCTGT TCTGACCACC TCTCGAGACT ATCGCAACAT CATTGAAAGA 
CTACAGGATA TAGTGTCTGC ATTGGAGGAG CGGTTGAGGC CTCTGGTTCA GGCTGAGTTG 
TCAGTGTTGG TGGATGTTCT CCATCGCCCA GAGCTGCTGT TTCCAGAGCA CACTGAAGCC 
CACCGCAAGT GTGAGAGTGG AGGCTTCATC TGCAAGTTGA TAAAACACAC TAAGCAGCTT 
CTAGAAGAAA ACGAAGAGAG GCTTTGCATC AAAGTCCTTC AGACACTCCG AGAGATGATG 
ACCAAAGACA GAGGATATGG AGAGAAGCTT CTGGGATATG ATGATGAGAT GGATGTCACG 
GAGGTGGTGG ATATCAGTCT GCCACCCAAA CAGCAGGAGG ATCGGCAGAG GGGGGAGGCC 
CTTCGGCAGC TGCTCGTCAA TCGTTACTAT GGCTTCAGGA GTGGTGGGCG GAGAGAAAGT 
CTGACCTCGT TTGGTAACTC CACCCTAACT CCTGTCGGGC CAATCAAGAG CCAGCCAGGG 
GGGTTGAGCA GGGCTGAGAT GTCATTAATG GAGGTGCAGT GTCATCTAGA CCGTGAAGGG 
GCATCGGATC TTGTCATTGA TCTCATCATG AACACTACCA GCGACCGCGT CTTCCATGAA 
AGCATTCTGT TGGCCATCGC ACTGTTGGAG GGCGGAAACA CCATCATACA GCATTCCTTC 
TTCAAACGTT TAACAGAAGA CAAAAATTCA GAGAAGTTTT TCAGGGTGTT TTATGACAGA 
ATGAAAGTGG CTCAGGTGGA GATCAAAGCC ACGGTTACTG TCAACACTAG TGACCTCGGC 
AACAAGCGGC GTGACGACAA CACTCCAGAC AAAGATACTC CACAACGCCG CAGAGGTAAA 
GACTCCGGTG TGGTGGTGAC AGATGATGCT CGTGAGCAGC TGTTGGAGGC TTCAGCTGCG 
ACTAAGAAGG CCTTTGGTTC ATATCGTCGA GATGCTGATC CCGAGGAGAC GTTTGGCACG 
GCTGATGGAG ATAAAGGAGG TGGAGATAAA GGCACAGAGC AGGGCGAGAT GAGCCCTGTC 
ATCCTCATAA TGCAGCCAAT CCTGCGCTTC CTGCAGCTGC TGTGCGAAAA CCACAATCGA 
GACCTGCAGA ATTTTCTGCG CTGTCAGAAC AATAAAAACA ACTATAATCT GGTGTGTGAG 
ACGCTGCAGT TCCTGGACTG TATCTGTGGC AGCACAACAG GAGGCCTGGG GCTGTTAGGA 
CTCTACATCA ACCAGCATAA CGTAGCGCTC ATTAATCAGA CTGTAGAGAG CCTCACTGAA 
TACTGTCAAG GTCCCTGCCA TGACAATCAG AATTGCATTG CCACCCATGA GTCTAATGGC 
ATCGACATCA TTATTGCACT GATTCTGAAT GATATTAATC CACTGGGCAG GAAGAGAATG 
GATCTGGTTC TAGAACTAAA GAATAATGCA TCTAAGCTGC TGCTGGCCAT CATGGAGAGC 
CGTCATGACA GTGAGAATGC AGAAAGAATT CTGTACAACA TGAGACCAAA AGAACTAGTG 
GAGGTGATGA AAATGGCCTA CCAGCAGGGG GAGGCAGAGT TTGAGGATGA GGAACAGGAG 
AATGGAGAGG ATCATGCAGC ATCGCCTCGC AATGTTGGAC ACAACATCTA TATTCTTGCA 
CATCAGCTGT CGCGTCACAA TAAGGAATTG CAAATACTAC TGAAACCAGG TGGAGAGGAC 
CAGGCCCTGG AGTATTACAC AAAACACACC TCACAGATAG AGATAGTTCG ACAGGATCGC 
ACTATGGAAC AGATTGTGTT TCCTGTACCA AATATCTGCT CTTTTCTGAC AAATGAGTCC 
AAGCTGCGTG TGTACTATGG TACAGAGAGA GACGAGCAAG GCAGTAAAAT AAATGACTTC 
TTCTTGCATG CAGACGACCT CTTTAATGAA ATGAGGTGGC AGAAAAAACT AAGAGCTCAG 
CCAGTGCTGT ACTGGTGCAG CAGGAACATG TCGTTTTGGA GTAACGTTTC CTTTAATCTG 
GCGGTGCTCA TTAATTTGCT GGTGGCATTT TTCTACCCTC TGGATGGAGT GAGCGAAAGC 
CAGCTGGAAC CTTCTCTGTC TCTGTTGCTG TGGGTTTGTT TGTTGGGCTC ACTGGGCTTC 
GTGTTGATGT CCCCGAGGCC AAATGCAGTG CGGGTGCTGG TCATCTCCAC TGTCCTGCAG 
TTGGGCTTCT CTGTAGGGCT GCAGCACATG CTCACACTCC TGGGAGCTTT CAATGTTTGT 
AATAAGATCG TGTTCATGCT GAGCTTTGTG GGTAACCGTG GGTCATTCAC CCGGGGTTAT 
CGGGTGATGG TCATGGATAG AGAGTTCCTG TTTCATCTGC TGTACCTTCT CATCTGCATA 
CTGGGCCTTT TTGGACACGT CTTCTTTTAC AGTCTTCTGT TGTTTGACCT GGTGAACCGG 
GAAGAGACTC TTCTGAACGT CATCAAGAGT GTGACTCGAA ACGGCCGCTC CATCGTCTTG 
ACCGCAGTGC TGGGCCTCAT CCTGGTCTAC CTCTTTTCTA TTGTTGGATA TATGTTTTTC 
AAAGATGACT TCATCCTGGA GGTGAACCGC ATCTCCAACG GCACTCTGGA GGAGGGGGTG 
ACTTTACCTG CTGGTTTTTG TGATGCGAGA AACGGGTCGT GTCTTTCTGA TGATGTTGAC 
GAGGATGACG TGGAGCGCGC GTGTGATTCT CTATGGATGT GTATGATCAC TGTACTCAGT 
CATGGCCTCA GGAGCGGTGG AGGAGTTGGA GATGTCCTGC GCAAACCGTC TAAAGAGGAG 
CGTCTGTTCG CCGCACGGGT GGTGTACGAT CTCCTGTTCT TCTTCCTGGT GATCATCATT 
GTGTTGAATC TGATCTTCGG TGTGATCATT GACACATTTG CAGATCTGAG AAGCGAGAAG 
CAGAGAAAAG AGGAGATCTT GAAGACCACA TGCTTCATCT GTGGTCTGGA GAGAGATAAG 
TTTGATAATA AAACGGTGAC GTTTGAAGAG CACATCAAGG AGGAGCACAA CCTGTGGCAT 
TACCTCTTCT TCATAGTGCT GGTGCGGGTC AAAGACTCCA CCGAATACAC GGGACCCGAG 
AGCTACGTCG CTCAGATGAT CAAGGAGCAC AATCTGGACT GGTTTCCTCG TATGAGGGCG 
ATGTCTCTGG TCAGCAGTGA TGGAGAAGGT GAACAGAATG AACTGCGCAG CTTACAGGAG 
AAACTCGAGT CCACAATGAG GCTCGTCTCT AATCTTACCA ATCAGCTCAC AGAGCTCAAA 
GAGCAGATGA CTGAGCAAAG GAAACACAAG CAGAGGCTCG GTCTGCTGGG AAACCCCGCC 
CACCTCAACA TCAACCCACA GCAGCCAGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300860.1
CDS	148..8280
Translation	MVDKLSSFLHIGDVCSLYAEGSTSGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGTSTTDTVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDAAGNEGSWFYIQPFYKLRSIGDNVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCTTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDEHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEVNPDYEEECLESRSSMDSEHEVIRARARNPQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSPLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAAKLEKGTITQNERRSVTKLLEDLVYFVVDIPSSAQDVLEITVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRYSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDPANADILIETKLVLSRFEVAGTVLGEGAEEEEEDEEEVWLFWKDSGGEVKSKSIRELAQDAKDGHTEDQEVINYYRYQLNLFARMCLDRQYLAINKISAQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSQISIDDYDNDGTSSDEVKERFSQTMEFVENYLRDVVCQSFPFSDKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHISTPFHIKLDREPGKGSNVMRSIHGVGELMTQVVLRGGGFLPASSHNPPDRDEVKAQSEPQKQDILVMDTKLKIIEILQFILNVRLDYRISCLLCIFKTEFDESNSQSEPSLSPESPASVQGALDFEHIEEQAEGIFGGSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALHLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSDSGLDAGEVTPEAHHKGVINSSRSNKPKVESTSSSNYRVVKEILLRLSKLCVLEGISGKKNKKQQQRLLRNMGAHSVVLELLQIPYEKGEDVQMQEIMTLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAVTMQHIFMNNFQLCSEINERVVQHFVHCIETHGRSVHYLKFLQTIVKAENKFIKKCQDIVMAELVTAGEDVLVFYNDRASFQSLVQMMRLERERLDESSALRYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKMAYVNFLNHCYVDTEVEMKEIYTSNHMWKLFDDFLVDVCRVCNNTSDRKHADTVLERYVTETIMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQGVFRVYHCNWLLPGQKGNVEACIKVLSDVAKGRAIAIPVDLDCQVNNLFMKSNNIVQKTALSWRLSVRNATRRDSVLTTSRDYRNIIERLQDIVSALEERLRPLVQAELSVLVDVLHRPELLFPEHTEAHRKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKLLGYDDEMDVTEVVDISLPPKQQEDRQRGEALRQLLVNRYYGFRSGGRRESLTSFGNSTLTPVGPIKSQPGGLSRAEMSLMEVQCHLDREGASDLVIDLIMNTTSDRVFHESILLAIALLEGGNTIIQHSFFKRLTEDKNSEKFFRVFYDRMKVAQVEIKATVTVNTSDLGNKRRDDNTPDKDTPQRRRGKDSGVVVTDDAREQLLEASAATKKAFGSYRRDADPEETFGTADGDKGGGDKGTEQGEMSPVILIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINQHNVALINQTVESLTEYCQGPCHDNQNCIATHESNGIDIIIALILNDINPLGRKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVMKMAYQQGEAEFEDEEQENGEDHAASPRNVGHNIYILAHQLSRHNKELQILLKPGGEDQALEYYTKHTSQIEIVRQDRTMEQIVFPVPNICSFLTNESKLRVYYGTERDEQGSKINDFFLHADDLFNEMRWQKKLRAQPVLYWCSRNMSFWSNVSFNLAVLINLLVAFFYPLDGVSESQLEPSLSLLLWVCLLGSLGFVLMSPRPNAVRVLVISTVLQLGFSVGLQHMLTLLGAFNVCNKIVFMLSFVGNRGSFTRGYRVMVMDREFLFHLLYLLICILGLFGHVFFYSLLLFDLVNREETLLNVIKSVTRNGRSIVLTAVLGLILVYLFSIVGYMFFKDDFILEVNRISNGTLEEGVTLPAGFCDARNGSCLSDDVDEDDVERACDSLWMCMITVLSHGLRSGGGVGDVLRKPSKEERLFAARVVYDLLFFFLVIIIVLNLIFGVIIDTFADLRSEKQRKEEILKTTCFICGLERDKFDNKTVTFEEHIKEEHNLWHYLFFIVLVRVKDSTEYTGPESYVAQMIKEHNLDWFPRMRAMSLVSSDGEGEQNELRSLQEKLESTMRLVSNLTNQLTELKEQMTEQRKHKQRLGLLGNPAHLNINPQQPA

Target ORF information:

RefSeq Version	XM_009302585.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302585.2

ORF Insert Sequence:

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GGTAAATCCA 
GACTATGAAG AAGAATGCCT GGAGTCCCGC TCCTCTATGG ATTCGGAGCA TGAAGTGATT 
CGGGCTCGTG CACGTAACCC TCAGGATAAG GTGATGTACA CACTGGTGTC TGTTCCTGAT 
GGAAATGACA TATCCTCCAT CTTTGAGCTT GACCCTACAA CTTTGAGGGG CGGAGACTCA 
CTTGTGCCAA GAAACTCGTA TGTGAGGCTC AGGCATCTCT GCACCAACAC GTGGGTCCAC 
AGCACCAACC AGCCAATAGA TAAAGAGGAA GAGAAACCGG TCATGCTCAG AATAGGAACG 
TCTCCTCTGA AGGAAGATAA AGAGGCGTTT GCTATAGTAC CAGTCTCTCC AGCTGAGGTT 
CGAGATCTGG ATTTTGCTAA TGATGCTAGT AAAGTGTTGG CTTCCATTGC TGCCAAACTG 
GAGAAGGGCA CCATTACCCA GAATGAGAGG AGGTCAGTGA CGAAGCTGTT GGAGGATCTG 
GTGTATTTTG TGGTGGACAT ACCCAGCAGT GCTCAAGATG TGCTGGAGAT CACAGTCAAC 
AAACCCAACA GAGAGCGACA GAAACTCATG AGAGAGCAGA ACATTCTTAA ACAGATCTTC 
AAGCTGCTGC AGGCGCCATT TACAGACAGC GGGGACGGCC CTATGTTGCG ATTAGAAGAG 
CTCGCAGACC AGCGTCATGC ACCCTTTCGA CACATTTGCA GACTCTGTTA CAGAGTCCTA 
CGTTACTCAC AACAGGACTA TCGCAAAAAT CAGGAGTACA TAGCAAAGCA GTTCCGCTTC 
ATGCAGAAGC AGATAGGATA TGATGTGCTG GCTGAAGACA CAATTACAGC CCTTCTGCAC 
AACAACAGGA AGCTCCTAGA GAAACACATC ACAGCAGCTG AAATAGATAC CTTTGTCAGT 
CTGGTCCGCA AGAACAGAGA GCCCAGATTT CTGGATTACT TGTCAGATCT GTGTGTGTCG 
ATGAATAAAT CCATCCCGGT CACTCAAGAG CTCATCTGTA ATGCTGTGCT GGACCCAGCC 
AATGCAGACA TTCTCATCGA AACCAAGCTT GTGTTGTCAC GGTTTGAGGT GGCAGGAACG 
GTGTTGGGTG AAGGTGCGGA GGAGGAAGAG GAGGATGAAG AGGAGGTCTG GCTGTTCTGG 
AAGGACAGTG GAGGAGAGGT GAAGAGTAAA AGCATACGAG AGCTTGCACA GGACGCAAAA 
GATGGACATA CAGAGGACCA GGAAGTCATC AATTATTACA GATATCAGCT GAATTTGTTT 
GCTCGGATGT GTCTGGATCG ACAGTATCTT GCGATTAACA AGATATCAGC ACAGCTGGAT 
GTGGATCTGA TTCTGCGCTG CATGTCAGAT GAAGATTTGC CCTTTGACCT CAGAGCATCG 
TTCTGCCGCA TGATGCTGCA CATGCATGTA GACCGTGACC CTCAGGAGCA GGTCACCCCG 
GTCAAATATG CTCGACTGTG GTCTGAAATC CCATCACAAA TTTCCATTGA TGATTATGAT 
AATGATGGGA CGTCAAGTGA TGAAGTGAAA GAGCGCTTCT CTCAGACGAT GGAGTTTGTG 
GAGAATTACC TGAGGGATGT TGTCTGCCAG AGTTTCCCGT TCTCTGATAA GGAGAAAAAC 
AAACTCACTT TTGAGGTGGT GAACCTGGCA AGAAACCTGA TCTATTTTGG TTTCTATAAC 
TTCAGTGATC TGCTAAGGTT AACAAAAATC CTATTGGCAA TCCTGGACTG TGTCCACATA 
AGCACTCCCT TCCACATCAA GCTGGATAGA GAACCCGGTA AAGGAAGTAA CGTGATGCGC 
TCCATCCATG GGGTGGGAGA GCTGATGACA CAGGTGGTGT TGAGGGGTGG GGGATTTCTG 
CCAGCCTCCA GCCACAATCC TCCTGACAGA GACGAAGTGA AAGCACAAAG TGAACCTCAG 
AAACAGGACA TACTGGTGAT GGACACTAAA CTCAAGATCA TCGAGATCCT GCAGTTTATT 
CTGAATGTGC GGTTGGACTA CAGGATCTCC TGCCTCCTCT GTATCTTTAA GACAGAGTTT 
GATGAAAGTA ACTCTCAAAG TGAACCATCT TTAAGCCCAG AATCTCCTGC CAGCGTTCAA 
GGTGCGCTAG ACTTTGAGCA CATTGAAGAG CAGGCGGAGG GTATTTTTGG AGGAAGTGAA 
GAGAACACCC CGCTGGATCT TGATGATCAT GGTGGACGGA CATTTCTGAG GGTCCTGCTT 
CACCTGACCA TGCATGACTA TCCTCCGCTG GTATCTCGAG CTCTTCACCT GCTCTTCAGA 
CACTTTAGCC AACGCCAGGA GGTCCTACAG GCCTTCAAAC AGGTTCAGCT CCTGGTGACC 
AGTCAGGATG TGGAAAACTA TAAACAGATC AAGTCAGATT TGGACCAGCT GAGGTCAATA 
GTAGAGAAAT CAGAGTTGTG GGTGTATAAG AGACAAGGCT CAGACTCAGG ACTGGATGCT 
GGAGAAGTCA CCCCTGAAGC CCACCACAAG GGTGTCATCA ACTCGAGTAG ATCAAACAAA 
CCCAAAGTGG AAAGTACCAG CAGCTCGAAC TACAGAGTCG TGAAAGAGAT CCTGCTGAGG 
CTGAGCAAAC TGTGTGTTTT GGAGGGCATC TCTGGCAAGA AGAATAAGAA ACAGCAGCAG 
CGGCTCCTCA GGAACATGGG AGCTCACAGT GTGGTCCTCG AACTGTTGCA AATCCCTTAT 
GAGAAGGGGG AGGATGTCCA GATGCAGGAG ATCATGACCT TAGCACATCA GTTCCTTCAA 
AACTTCTGTG CTGGAAATCA GCAGAACCAG GCACTTCTAC ACAAACACAT CAACCTGTTC 
CTCAACCCTG GGATCCTGGA GGCGGTGACC ATGCAGCATA TCTTCATGAA TAATTTCCAG 
TTGTGTAGTG AGATTAATGA GCGGGTCGTG CAGCATTTTG TGCACTGCAT CGAGACACAC 
GGCCGCAGCG TGCACTACCT CAAGTTCCTG CAGACCATTG TCAAGGCAGA GAACAAGTTC 
ATCAAGAAAT GCCAAGACAT CGTCATGGCT GAGCTGGTGA CTGCAGGTGA GGATGTGCTG 
GTGTTCTATA ATGACCGGGC GTCTTTTCAG TCATTGGTTC AGATGATGCG TCTTGAGCGG 
GAACGACTTG ATGAGAGCAG TGCACTCAGG TATCACATTC ACCTGGTGGA GTTGCTGGCT 
GTGTGCACTG AAGGCAAGAA CGTCTACACT GAGATCAAAT GCAACTCGCT GCTCCCTCTC 
GATGATATTG TACGTGTGGT CACACATGAG GACTGCATCC CTGAGGTGAA GATGGCCTAC 
GTGAACTTCC TGAATCACTG TTATGTTGAT ACAGAGGTTG AGATGAAGGA GATCTACACC 
AGCAATCACA TGTGGAAACT TTTTGATGAT TTTCTGGTGG ACGTCTGCAG AGTGTGCAAT 
AACACAAGTG ACCGTAAACA CGCTGACACA GTTCTGGAGA GATACGTTAC AGAGACCATA 
ATGAGCATCG TCACCACCTT TTTCAGCTCA CCATTCTCAG ACCAGAGCAC AAGTTTGCAG 
ACCCGGCAGC CGGTGTTTGT GCAGCTGCTG CAGGGAGTGT TTCGTGTATA TCACTGCAAC 
TGGCTTCTCC CAGGTCAGAA GGGGAATGTG GAAGCCTGCA TCAAAGTGCT GAGTGACGTG 
GCCAAAGGCA GAGCCATAGC CATTCCTGTG GATCTGGACT GTCAGGTGAA TAATCTGTTC 
ATGAAGTCCA ACAACATTGT GCAGAAAACA GCCCTCAGCT GGAGACTCTC TGTCCGTAAC 
GCGACTCGGA GAGACTCTGT TCTGACCACC TCTCGAGACT ATCGCAACAT CATTGAAAGA 
CTACAGGATA TAGTGTCTGC ATTGGAGGAG CGGTTGAGGC CTCTGGTTCA GGCTGAGTTG 
TCAGTGTTGG TGGATGTTCT CCATCGCCCA GAGCTGCTGT TTCCAGAGCA CACTGAAGCC 
CACCGCAAGT GTGAGAGTGG AGGCTTCATC TGCAAGTTGA TAAAACACAC TAAGCAGCTT 
CTAGAAGAAA ACGAAGAGAG GCTTTGCATC AAAGTCCTTC AGACACTCCG AGAGATGATG 
ACCAAAGACA GAGGATATGG AGAGAAGCTT CTGGGATATG ATGATGAGAT GGATGTCACG 
GAGGTGGTGG ATATCAGTCT GCCACCCAAA CAGCAGGAGG ATCGGCAGAG GGGGGAGGCC 
CTTCGGCAGC TGCTCGTCAA TCGTTACTAT GGCTTCAGGA GTGGTGGGCG GAGAGAAAGT 
CTGACCTCGT TTGGTAACTC CACCCTAACT CCTGTCGGGC CAATCAAGAG CCAGCCAGGG 
GGGTTGAGCA GGGCTGAGAT GTCATTAATG GAGGTGCAGT GTCATCTAGA CCGTGAAGGG 
GCATCGGATC TTGTCATTGA TCTCATCATG AACACTACCA GCGACCGCGT CTTCCATGAA 
AGCATTCTGT TGGCCATCGC ACTGTTGGAG GGCGGAAACA CCATCATACA GCATTCCTTC 
TTCAAACGTT TAACAGAAGA CAAAAATTCA GAGAAGTTTT TCAGGGTGTT TTATGACAGA 
ATGAAAGTGG CTCAGGTGGA GATCAAAGCC ACGGTTACTG TCAACACTAG TGACCTCGGC 
AACAAGCGGC GTGACGACAA CACTCCAGAC AAAGATACTC CACAACGCCG CAGAGGTAAA 
GACTCCGGTG TGGTGGTGAC AGATGATGCT CGTGAGCAGC TGTTGGAGGC TTCAGCTGCG 
ACTAAGAAGG CCTTTGGTTC ATATCGTCGA GATGCTGATC CCGAGGAGAC GTTTGGCACG 
GCTGATGGAG ATAAAGGAGG TGGAGATAAA GGCACAGAGC AGGGCGAGAT GAGCCCTGTC 
ATCCTCATAA TGCAGCCAAT CCTGCGCTTC CTGCAGCTGC TGTGCGAAAA CCACAATCGA 
GACCTGCAGA ATTTTCTGCG CTGTCAGAAC AATAAAAACA ACTATAATCT GGTGTGTGAG 
ACGCTGCAGT TCCTGGACTG TATCTGTGGC AGCACAACAG GAGGCCTGGG GCTGTTAGGA 
CTCTACATCA ACCAGCATAA CGTAGCGCTC ATTAATCAGA CTGTAGAGAG CCTCACTGAA 
TACTGTCAAG GTCCCTGCCA TGACAATCAG AATTGCATTG CCACCCATGA GTCTAATGGC 
ATCGACATCA TTATTGCACT GATTCTGAAT GATATTAATC CACTGGGCAG GAAGAGAATG 
GATCTGGTTC TAGAACTAAA GAATAATGCA TCTAAGCTGC TGCTGGCCAT CATGGAGAGC 
CGTCATGACA GTGAGAATGC AGAAAGAATT CTGTACAACA TGAGACCAAA AGAACTAGTG 
GAGGTGATGA AAATGGCCTA CCAGCAGGGG GAGGCAGAGT TTGAGGATGA GGAACAGGAG 
AATGGAGAGG ATCATGCAGC ATCGCCTCGC AATGTTGGAC ACAACATCTA TATTCTTGCA 
CATCAGCTGT CGCGTCACAA TAAGGAATTG CAAATACTAC TGAAACCAGG TGGAGAGGAC 
CAGGCCCTGG AGTATTACAC AAAACACACC TCACAGATAG AGATAGTTCG ACAGGATCGC 
ACTATGGAAC AGATTGTGTT TCCTGTACCA AATATCTGCT CTTTTCTGAC AAATGAGTCC 
AAGCTGCGTG TGTACTATGG TACAGAGAGA GACGAGCAAG GCAGTAAAAT AAATGACTTC 
TTCTTGCATG CAGACGACCT CTTTAATGAA ATGAGGTGGC AGAAAAAACT AAGAGCTCAG 
CCAGTGCTGT ACTGGTGCAG CAGGAACATG TCGTTTTGGA GTAACGTTTC CTTTAATCTG 
GCGGTGCTCA TTAATTTGCT GGTGGCATTT TTCTACCCTC TGGATGGAGT GAGCGAAAGC 
CAGCTGGAAC CTTCTCTGTC TCTGTTGCTG TGGGTTTGTT TGTTGGGCTC ACTGGGCTTC 
GTGTTGATGT CCCCGAGGCC AAATGCAGTG CGGGTGCTGG TCATCTCCAC TGTCCTGCAG 
TTGGGCTTCT CTGTAGGGCT GCAGCACATG CTCACACTCC TGGGAGCTTT CAATGTTTGT 
AATAAGATCG TGTTCATGCT GAGCTTTGTG GGTAACCGTG GGTCATTCAC CCGGGGTTAT 
CGGGTGATGG TCATGGATAG AGAGTTCCTG TTTCATCTGC TGTACCTTCT CATCTGCATA 
CTGGGCCTTT TTGGACACGT CTTCTTTTAC AGTCTTCTGT TGTTTGACCT GGTGAACCGG 
GAAGAGACTC TTCTGAACGT CATCAAGAGT GTGACTCGAA ACGGCCGCTC CATCGTCTTG 
ACCGCAGTGC TGGGCCTCAT CCTGGTCTAC CTCTTTTCTA TTGTTGGATA TATGTTTTTC 
AAAGATGACT TCATCCTGGA GGTGAACCGC ATCTCCAACG GCACTCTGGA GGAGGGGGTG 
ACTTTACCTG CTGGTTTTTG TGATGCGAGA AACGGGTCGT GTCTTTCTGA TGATGTTGAC 
GAGGATGACG TGGAGCGCGC GTGTGATTCT CTATGGATGT GTATGATCAC TGTACTCAGT 
CATGGCCTCA GGAGCGGTGG AGGAGTTGGA GATGTCCTGC GCAAACCGTC TAAAGAGGAG 
CGTCTGTTCG CCGCACGGGT GGTGTACGAT CTCCTGTTCT TCTTCCTGGT GATCATCATT 
GTGTTGAATC TGATCTTCGG TGTGATCATT GACACATTTG CAGATCTGAG AAGCGAGAAG 
CAGAGAAAAG AGGAGATCTT GAAGACCACA TGCTTCATCT GTGGTCTGGA GAGAGATAAG 
TTTGATAATA AAACGGTGAC GTTTGAAGAG CACATCAAGG AGGAGCACAA CCTGTGGCAT 
TACCTCTTCT TCATAGTGCT GGTGCGGGTC AAAGACTCCA CCGAATACAC GGGACCCGAG 
AGCTACGTCG CTCAGATGAT CAAGGAGCAC AATCTGGACT GGTTTCCTCG TATGAGGGCG 
ATGTCTCTGG TCAGCAGTGA TGGAGAAGGT GAACAGAATG AACTGCGCAG CTTACAGGAG 
AAACTCGAGT CCACAATGAG GCTCGTCTCT AATCTTACCA ATCAGCTCAC AGAGCTCAAA 
GAGCAGATGA CTGAGCAAAG GAAACACAAG CAGAGGCTCG GTCTGCTGGG AAACCCCGCC 
CACCTCAACA TCAACCCACA GCAGCCAGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itpr1a ( XM_009302585.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300860.1 Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X2, mRNA.

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CloneID	ODa45192
Clone ID Related Accession (Same CDS sequence)	XM_009302585.2 , XM_009302585.3
Accession Version	XM_009302585.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8133bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009302585.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GGTAAATCCA 
GACTATGAAG AAGAATGCCT GGAGTCCCGC TCCTCTATGG ATTCGGAGCA TGAAGTGATT 
CGGGCTCGTG CACGTAACCC TCAGGATAAG GTGATGTACA CACTGGTGTC TGTTCCTGAT 
GGAAATGACA TATCCTCCAT CTTTGAGCTT GACCCTACAA CTTTGAGGGG CGGAGACTCA 
CTTGTGCCAA GAAACTCGTA TGTGAGGCTC AGGCATCTCT GCACCAACAC GTGGGTCCAC 
AGCACCAACC AGCCAATAGA TAAAGAGGAA GAGAAACCGG TCATGCTCAG AATAGGAACG 
TCTCCTCTGA AGGAAGATAA AGAGGCGTTT GCTATAGTAC CAGTCTCTCC AGCTGAGGTT 
CGAGATCTGG ATTTTGCTAA TGATGCTAGT AAAGTGTTGG CTTCCATTGC TGCCAAACTG 
GAGAAGGGCA CCATTACCCA GAATGAGAGG AGGTCAGTGA CGAAGCTGTT GGAGGATCTG 
GTGTATTTTG TGGTGGACAT ACCCAGCAGT GCTCAAGATG TGCTGGAGAT CACAGTCAAC 
AAACCCAACA GAGAGCGACA GAAACTCATG AGAGAGCAGA ACATTCTTAA ACAGATCTTC 
AAGCTGCTGC AGGCGCCATT TACAGACAGC GGGGACGGCC CTATGTTGCG ATTAGAAGAG 
CTCGCAGACC AGCGTCATGC ACCCTTTCGA CACATTTGCA GACTCTGTTA CAGAGTCCTA 
CGTTACTCAC AACAGGACTA TCGCAAAAAT CAGGAGTACA TAGCAAAGCA GTTCCGCTTC 
ATGCAGAAGC AGATAGGATA TGATGTGCTG GCTGAAGACA CAATTACAGC CCTTCTGCAC 
AACAACAGGA AGCTCCTAGA GAAACACATC ACAGCAGCTG AAATAGATAC CTTTGTCAGT 
CTGGTCCGCA AGAACAGAGA GCCCAGATTT CTGGATTACT TGTCAGATCT GTGTGTGTCG 
ATGAATAAAT CCATCCCGGT CACTCAAGAG CTCATCTGTA ATGCTGTGCT GGACCCAGCC 
AATGCAGACA TTCTCATCGA AACCAAGCTT GTGTTGTCAC GGTTTGAGGT GGCAGGAACG 
GTGTTGGGTG AAGGTGCGGA GGAGGAAGAG GAGGATGAAG AGGAGGTCTG GCTGTTCTGG 
AAGGACAGTG GAGGAGAGGT GAAGAGTAAA AGCATACGAG AGCTTGCACA GGACGCAAAA 
GATGGACATA CAGAGGACCA GGAAGTCATC AATTATTACA GATATCAGCT GAATTTGTTT 
GCTCGGATGT GTCTGGATCG ACAGTATCTT GCGATTAACA AGATATCAGC ACAGCTGGAT 
GTGGATCTGA TTCTGCGCTG CATGTCAGAT GAAGATTTGC CCTTTGACCT CAGAGCATCG 
TTCTGCCGCA TGATGCTGCA CATGCATGTA GACCGTGACC CTCAGGAGCA GGTCACCCCG 
GTCAAATATG CTCGACTGTG GTCTGAAATC CCATCACAAA TTTCCATTGA TGATTATGAT 
AATGATGGGA CGTCAAGTGA TGAAGTGAAA GAGCGCTTCT CTCAGACGAT GGAGTTTGTG 
GAGAATTACC TGAGGGATGT TGTCTGCCAG AGTTTCCCGT TCTCTGATAA GGAGAAAAAC 
AAACTCACTT TTGAGGTGGT GAACCTGGCA AGAAACCTGA TCTATTTTGG TTTCTATAAC 
TTCAGTGATC TGCTAAGGTT AACAAAAATC CTATTGGCAA TCCTGGACTG TGTCCACATA 
AGCACTCCCT TCCACATCAA GCTGGATAGA GAACCCGGTA AAGGAAGTAA CGTGATGCGC 
TCCATCCATG GGGTGGGAGA GCTGATGACA CAGGTGGTGT TGAGGGGTGG GGGATTTCTG 
CCAGCCTCCA GCCACAATCC TCCTGACAGA GACGAAGTGA AAGCACAAAG TGAACCTCAG 
AAACAGGACA TACTGGTGAT GGACACTAAA CTCAAGATCA TCGAGATCCT GCAGTTTATT 
CTGAATGTGC GGTTGGACTA CAGGATCTCC TGCCTCCTCT GTATCTTTAA GACAGAGTTT 
GATGAAAGTA ACTCTCAAAG TGAACCATCT TTAAGCCCAG AATCTCCTGC CAGCGTTCAA 
GGTGCGCTAG ACTTTGAGCA CATTGAAGAG CAGGCGGAGG GTATTTTTGG AGGAAGTGAA 
GAGAACACCC CGCTGGATCT TGATGATCAT GGTGGACGGA CATTTCTGAG GGTCCTGCTT 
CACCTGACCA TGCATGACTA TCCTCCGCTG GTATCTCGAG CTCTTCACCT GCTCTTCAGA 
CACTTTAGCC AACGCCAGGA GGTCCTACAG GCCTTCAAAC AGGTTCAGCT CCTGGTGACC 
AGTCAGGATG TGGAAAACTA TAAACAGATC AAGTCAGATT TGGACCAGCT GAGGTCAATA 
GTAGAGAAAT CAGAGTTGTG GGTGTATAAG AGACAAGGCT CAGACTCAGG ACTGGATGCT 
GGAGAAGTCA CCCCTGAAGC CCACCACAAG GGTGTCATCA ACTCGAGTAG ATCAAACAAA 
CCCAAAGTGG AAAGTACCAG CAGCTCGAAC TACAGAGTCG TGAAAGAGAT CCTGCTGAGG 
CTGAGCAAAC TGTGTGTTTT GGAGGGCATC TCTGGCAAGA AGAATAAGAA ACAGCAGCAG 
CGGCTCCTCA GGAACATGGG AGCTCACAGT GTGGTCCTCG AACTGTTGCA AATCCCTTAT 
GAGAAGGGGG AGGATGTCCA GATGCAGGAG ATCATGACCT TAGCACATCA GTTCCTTCAA 
AACTTCTGTG CTGGAAATCA GCAGAACCAG GCACTTCTAC ACAAACACAT CAACCTGTTC 
CTCAACCCTG GGATCCTGGA GGCGGTGACC ATGCAGCATA TCTTCATGAA TAATTTCCAG 
TTGTGTAGTG AGATTAATGA GCGGGTCGTG CAGCATTTTG TGCACTGCAT CGAGACACAC 
GGCCGCAGCG TGCACTACCT CAAGTTCCTG CAGACCATTG TCAAGGCAGA GAACAAGTTC 
ATCAAGAAAT GCCAAGACAT CGTCATGGCT GAGCTGGTGA CTGCAGGTGA GGATGTGCTG 
GTGTTCTATA ATGACCGGGC GTCTTTTCAG TCATTGGTTC AGATGATGCG TCTTGAGCGG 
GAACGACTTG ATGAGAGCAG TGCACTCAGG TATCACATTC ACCTGGTGGA GTTGCTGGCT 
GTGTGCACTG AAGGCAAGAA CGTCTACACT GAGATCAAAT GCAACTCGCT GCTCCCTCTC 
GATGATATTG TACGTGTGGT CACACATGAG GACTGCATCC CTGAGGTGAA GATGGCCTAC 
GTGAACTTCC TGAATCACTG TTATGTTGAT ACAGAGGTTG AGATGAAGGA GATCTACACC 
AGCAATCACA TGTGGAAACT TTTTGATGAT TTTCTGGTGG ACGTCTGCAG AGTGTGCAAT 
AACACAAGTG ACCGTAAACA CGCTGACACA GTTCTGGAGA GATACGTTAC AGAGACCATA 
ATGAGCATCG TCACCACCTT TTTCAGCTCA CCATTCTCAG ACCAGAGCAC AAGTTTGCAG 
ACCCGGCAGC CGGTGTTTGT GCAGCTGCTG CAGGGAGTGT TTCGTGTATA TCACTGCAAC 
TGGCTTCTCC CAGGTCAGAA GGGGAATGTG GAAGCCTGCA TCAAAGTGCT GAGTGACGTG 
GCCAAAGGCA GAGCCATAGC CATTCCTGTG GATCTGGACT GTCAGGTGAA TAATCTGTTC 
ATGAAGTCCA ACAACATTGT GCAGAAAACA GCCCTCAGCT GGAGACTCTC TGTCCGTAAC 
GCGACTCGGA GAGACTCTGT TCTGACCACC TCTCGAGACT ATCGCAACAT CATTGAAAGA 
CTACAGGATA TAGTGTCTGC ATTGGAGGAG CGGTTGAGGC CTCTGGTTCA GGCTGAGTTG 
TCAGTGTTGG TGGATGTTCT CCATCGCCCA GAGCTGCTGT TTCCAGAGCA CACTGAAGCC 
CACCGCAAGT GTGAGAGTGG AGGCTTCATC TGCAAGTTGA TAAAACACAC TAAGCAGCTT 
CTAGAAGAAA ACGAAGAGAG GCTTTGCATC AAAGTCCTTC AGACACTCCG AGAGATGATG 
ACCAAAGACA GAGGATATGG AGAGAAGCTT CTGGGATATG ATGATGAGAT GGATGTCACG 
GAGGTGGTGG ATATCAGTCT GCCACCCAAA CAGCAGGAGG ATCGGCAGAG GGGGGAGGCC 
CTTCGGCAGC TGCTCGTCAA TCGTTACTAT GGCTTCAGGA GTGGTGGGCG GAGAGAAAGT 
CTGACCTCGT TTGGTAACTC CACCCTAACT CCTGTCGGGC CAATCAAGAG CCAGCCAGGG 
GGGTTGAGCA GGGCTGAGAT GTCATTAATG GAGGTGCAGT GTCATCTAGA CCGTGAAGGG 
GCATCGGATC TTGTCATTGA TCTCATCATG AACACTACCA GCGACCGCGT CTTCCATGAA 
AGCATTCTGT TGGCCATCGC ACTGTTGGAG GGCGGAAACA CCATCATACA GCATTCCTTC 
TTCAAACGTT TAACAGAAGA CAAAAATTCA GAGAAGTTTT TCAGGGTGTT TTATGACAGA 
ATGAAAGTGG CTCAGGTGGA GATCAAAGCC ACGGTTACTG TCAACACTAG TGACCTCGGC 
AACAAGCGGC GTGACGACAA CACTCCAGAC AAAGATACTC CACAACGCCG CAGAGGTAAA 
GACTCCGGTG TGGTGGTGAC AGATGATGCT CGTGAGCAGC TGTTGGAGGC TTCAGCTGCG 
ACTAAGAAGG CCTTTGGTTC ATATCGTCGA GATGCTGATC CCGAGGAGAC GTTTGGCACG 
GCTGATGGAG ATAAAGGAGG TGGAGATAAA GGCACAGAGC AGGGCGAGAT GAGCCCTGTC 
ATCCTCATAA TGCAGCCAAT CCTGCGCTTC CTGCAGCTGC TGTGCGAAAA CCACAATCGA 
GACCTGCAGA ATTTTCTGCG CTGTCAGAAC AATAAAAACA ACTATAATCT GGTGTGTGAG 
ACGCTGCAGT TCCTGGACTG TATCTGTGGC AGCACAACAG GAGGCCTGGG GCTGTTAGGA 
CTCTACATCA ACCAGCATAA CGTAGCGCTC ATTAATCAGA CTGTAGAGAG CCTCACTGAA 
TACTGTCAAG GTCCCTGCCA TGACAATCAG AATTGCATTG CCACCCATGA GTCTAATGGC 
ATCGACATCA TTATTGCACT GATTCTGAAT GATATTAATC CACTGGGCAG GAAGAGAATG 
GATCTGGTTC TAGAACTAAA GAATAATGCA TCTAAGCTGC TGCTGGCCAT CATGGAGAGC 
CGTCATGACA GTGAGAATGC AGAAAGAATT CTGTACAACA TGAGACCAAA AGAACTAGTG 
GAGGTGATGA AAATGGCCTA CCAGCAGGGG GAGGCAGAGT TTGAGGATGA GGAACAGGAG 
AATGGAGAGG ATCATGCAGC ATCGCCTCGC AATGTTGGAC ACAACATCTA TATTCTTGCA 
CATCAGCTGT CGCGTCACAA TAAGGAATTG CAAATACTAC TGAAACCAGG TGGAGAGGAC 
CAGGCCCTGG AGTATTACAC AAAACACACC TCACAGATAG AGATAGTTCG ACAGGATCGC 
ACTATGGAAC AGATTGTGTT TCCTGTACCA AATATCTGCT CTTTTCTGAC AAATGAGTCC 
AAGCTGCGTG TGTACTATGG TACAGAGAGA GACGAGCAAG GCAGTAAAAT AAATGACTTC 
TTCTTGCATG CAGACGACCT CTTTAATGAA ATGAGGTGGC AGAAAAAACT AAGAGCTCAG 
CCAGTGCTGT ACTGGTGCAG CAGGAACATG TCGTTTTGGA GTAACGTTTC CTTTAATCTG 
GCGGTGCTCA TTAATTTGCT GGTGGCATTT TTCTACCCTC TGGATGGAGT GAGCGAAAGC 
CAGCTGGAAC CTTCTCTGTC TCTGTTGCTG TGGGTTTGTT TGTTGGGCTC ACTGGGCTTC 
GTGTTGATGT CCCCGAGGCC AAATGCAGTG CGGGTGCTGG TCATCTCCAC TGTCCTGCAG 
TTGGGCTTCT CTGTAGGGCT GCAGCACATG CTCACACTCC TGGGAGCTTT CAATGTTTGT 
AATAAGATCG TGTTCATGCT GAGCTTTGTG GGTAACCGTG GGTCATTCAC CCGGGGTTAT 
CGGGTGATGG TCATGGATAG AGAGTTCCTG TTTCATCTGC TGTACCTTCT CATCTGCATA 
CTGGGCCTTT TTGGACACGT CTTCTTTTAC AGTCTTCTGT TGTTTGACCT GGTGAACCGG 
GAAGAGACTC TTCTGAACGT CATCAAGAGT GTGACTCGAA ACGGCCGCTC CATCGTCTTG 
ACCGCAGTGC TGGGCCTCAT CCTGGTCTAC CTCTTTTCTA TTGTTGGATA TATGTTTTTC 
AAAGATGACT TCATCCTGGA GGTGAACCGC ATCTCCAACG GCACTCTGGA GGAGGGGGTG 
ACTTTACCTG CTGGTTTTTG TGATGCGAGA AACGGGTCGT GTCTTTCTGA TGATGTTGAC 
GAGGATGACG TGGAGCGCGC GTGTGATTCT CTATGGATGT GTATGATCAC TGTACTCAGT 
CATGGCCTCA GGAGCGGTGG AGGAGTTGGA GATGTCCTGC GCAAACCGTC TAAAGAGGAG 
CGTCTGTTCG CCGCACGGGT GGTGTACGAT CTCCTGTTCT TCTTCCTGGT GATCATCATT 
GTGTTGAATC TGATCTTCGG TGTGATCATT GACACATTTG CAGATCTGAG AAGCGAGAAG 
CAGAGAAAAG AGGAGATCTT GAAGACCACA TGCTTCATCT GTGGTCTGGA GAGAGATAAG 
TTTGATAATA AAACGGTGAC GTTTGAAGAG CACATCAAGG AGGAGCACAA CCTGTGGCAT 
TACCTCTTCT TCATAGTGCT GGTGCGGGTC AAAGACTCCA CCGAATACAC GGGACCCGAG 
AGCTACGTCG CTCAGATGAT CAAGGAGCAC AATCTGGACT GGTTTCCTCG TATGAGGGCG 
ATGTCTCTGG TCAGCAGTGA TGGAGAAGGT GAACAGAATG AACTGCGCAG CTTACAGGAG 
AAACTCGAGT CCACAATGAG GCTCGTCTCT AATCTTACCA ATCAGCTCAC AGAGCTCAAA 
GAGCAGATGA CTGAGCAAAG GAAACACAAG CAGAGGCTCG GTCTGCTGGG AAACCCCGCC 
CACCTCAACA TCAACCCACA GCAGCCAGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300860.1
CDS	148..8280
Translation	MVDKLSSFLHIGDVCSLYAEGSTSGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGTSTTDTVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDAAGNEGSWFYIQPFYKLRSIGDNVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCTTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDEHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEVNPDYEEECLESRSSMDSEHEVIRARARNPQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSPLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAAKLEKGTITQNERRSVTKLLEDLVYFVVDIPSSAQDVLEITVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRYSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDPANADILIETKLVLSRFEVAGTVLGEGAEEEEEDEEEVWLFWKDSGGEVKSKSIRELAQDAKDGHTEDQEVINYYRYQLNLFARMCLDRQYLAINKISAQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSQISIDDYDNDGTSSDEVKERFSQTMEFVENYLRDVVCQSFPFSDKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHISTPFHIKLDREPGKGSNVMRSIHGVGELMTQVVLRGGGFLPASSHNPPDRDEVKAQSEPQKQDILVMDTKLKIIEILQFILNVRLDYRISCLLCIFKTEFDESNSQSEPSLSPESPASVQGALDFEHIEEQAEGIFGGSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALHLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSDSGLDAGEVTPEAHHKGVINSSRSNKPKVESTSSSNYRVVKEILLRLSKLCVLEGISGKKNKKQQQRLLRNMGAHSVVLELLQIPYEKGEDVQMQEIMTLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAVTMQHIFMNNFQLCSEINERVVQHFVHCIETHGRSVHYLKFLQTIVKAENKFIKKCQDIVMAELVTAGEDVLVFYNDRASFQSLVQMMRLERERLDESSALRYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKMAYVNFLNHCYVDTEVEMKEIYTSNHMWKLFDDFLVDVCRVCNNTSDRKHADTVLERYVTETIMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQGVFRVYHCNWLLPGQKGNVEACIKVLSDVAKGRAIAIPVDLDCQVNNLFMKSNNIVQKTALSWRLSVRNATRRDSVLTTSRDYRNIIERLQDIVSALEERLRPLVQAELSVLVDVLHRPELLFPEHTEAHRKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKLLGYDDEMDVTEVVDISLPPKQQEDRQRGEALRQLLVNRYYGFRSGGRRESLTSFGNSTLTPVGPIKSQPGGLSRAEMSLMEVQCHLDREGASDLVIDLIMNTTSDRVFHESILLAIALLEGGNTIIQHSFFKRLTEDKNSEKFFRVFYDRMKVAQVEIKATVTVNTSDLGNKRRDDNTPDKDTPQRRRGKDSGVVVTDDAREQLLEASAATKKAFGSYRRDADPEETFGTADGDKGGGDKGTEQGEMSPVILIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINQHNVALINQTVESLTEYCQGPCHDNQNCIATHESNGIDIIIALILNDINPLGRKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVMKMAYQQGEAEFEDEEQENGEDHAASPRNVGHNIYILAHQLSRHNKELQILLKPGGEDQALEYYTKHTSQIEIVRQDRTMEQIVFPVPNICSFLTNESKLRVYYGTERDEQGSKINDFFLHADDLFNEMRWQKKLRAQPVLYWCSRNMSFWSNVSFNLAVLINLLVAFFYPLDGVSESQLEPSLSLLLWVCLLGSLGFVLMSPRPNAVRVLVISTVLQLGFSVGLQHMLTLLGAFNVCNKIVFMLSFVGNRGSFTRGYRVMVMDREFLFHLLYLLICILGLFGHVFFYSLLLFDLVNREETLLNVIKSVTRNGRSIVLTAVLGLILVYLFSIVGYMFFKDDFILEVNRISNGTLEEGVTLPAGFCDARNGSCLSDDVDEDDVERACDSLWMCMITVLSHGLRSGGGVGDVLRKPSKEERLFAARVVYDLLFFFLVIIIVLNLIFGVIIDTFADLRSEKQRKEEILKTTCFICGLERDKFDNKTVTFEEHIKEEHNLWHYLFFIVLVRVKDSTEYTGPESYVAQMIKEHNLDWFPRMRAMSLVSSDGEGEQNELRSLQEKLESTMRLVSNLTNQLTELKEQMTEQRKHKQRLGLLGNPAHLNINPQQPA

Target ORF information:

RefSeq Version	XM_009302585.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302585.3

ORF Insert Sequence:

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GGTAAATCCA 
GACTATGAAG AAGAATGCCT GGAGTCCCGC TCCTCTATGG ATTCGGAGCA TGAAGTGATT 
CGGGCTCGTG CACGTAACCC TCAGGATAAG GTGATGTACA CACTGGTGTC TGTTCCTGAT 
GGAAATGACA TATCCTCCAT CTTTGAGCTT GACCCTACAA CTTTGAGGGG CGGAGACTCA 
CTTGTGCCAA GAAACTCGTA TGTGAGGCTC AGGCATCTCT GCACCAACAC GTGGGTCCAC 
AGCACCAACC AGCCAATAGA TAAAGAGGAA GAGAAACCGG TCATGCTCAG AATAGGAACG 
TCTCCTCTGA AGGAAGATAA AGAGGCGTTT GCTATAGTAC CAGTCTCTCC AGCTGAGGTT 
CGAGATCTGG ATTTTGCTAA TGATGCTAGT AAAGTGTTGG CTTCCATTGC TGCCAAACTG 
GAGAAGGGCA CCATTACCCA GAATGAGAGG AGGTCAGTGA CGAAGCTGTT GGAGGATCTG 
GTGTATTTTG TGGTGGACAT ACCCAGCAGT GCTCAAGATG TGCTGGAGAT CACAGTCAAC 
AAACCCAACA GAGAGCGACA GAAACTCATG AGAGAGCAGA ACATTCTTAA ACAGATCTTC 
AAGCTGCTGC AGGCGCCATT TACAGACAGC GGGGACGGCC CTATGTTGCG ATTAGAAGAG 
CTCGCAGACC AGCGTCATGC ACCCTTTCGA CACATTTGCA GACTCTGTTA CAGAGTCCTA 
CGTTACTCAC AACAGGACTA TCGCAAAAAT CAGGAGTACA TAGCAAAGCA GTTCCGCTTC 
ATGCAGAAGC AGATAGGATA TGATGTGCTG GCTGAAGACA CAATTACAGC CCTTCTGCAC 
AACAACAGGA AGCTCCTAGA GAAACACATC ACAGCAGCTG AAATAGATAC CTTTGTCAGT 
CTGGTCCGCA AGAACAGAGA GCCCAGATTT CTGGATTACT TGTCAGATCT GTGTGTGTCG 
ATGAATAAAT CCATCCCGGT CACTCAAGAG CTCATCTGTA ATGCTGTGCT GGACCCAGCC 
AATGCAGACA TTCTCATCGA AACCAAGCTT GTGTTGTCAC GGTTTGAGGT GGCAGGAACG 
GTGTTGGGTG AAGGTGCGGA GGAGGAAGAG GAGGATGAAG AGGAGGTCTG GCTGTTCTGG 
AAGGACAGTG GAGGAGAGGT GAAGAGTAAA AGCATACGAG AGCTTGCACA GGACGCAAAA 
GATGGACATA CAGAGGACCA GGAAGTCATC AATTATTACA GATATCAGCT GAATTTGTTT 
GCTCGGATGT GTCTGGATCG ACAGTATCTT GCGATTAACA AGATATCAGC ACAGCTGGAT 
GTGGATCTGA TTCTGCGCTG CATGTCAGAT GAAGATTTGC CCTTTGACCT CAGAGCATCG 
TTCTGCCGCA TGATGCTGCA CATGCATGTA GACCGTGACC CTCAGGAGCA GGTCACCCCG 
GTCAAATATG CTCGACTGTG GTCTGAAATC CCATCACAAA TTTCCATTGA TGATTATGAT 
AATGATGGGA CGTCAAGTGA TGAAGTGAAA GAGCGCTTCT CTCAGACGAT GGAGTTTGTG 
GAGAATTACC TGAGGGATGT TGTCTGCCAG AGTTTCCCGT TCTCTGATAA GGAGAAAAAC 
AAACTCACTT TTGAGGTGGT GAACCTGGCA AGAAACCTGA TCTATTTTGG TTTCTATAAC 
TTCAGTGATC TGCTAAGGTT AACAAAAATC CTATTGGCAA TCCTGGACTG TGTCCACATA 
AGCACTCCCT TCCACATCAA GCTGGATAGA GAACCCGGTA AAGGAAGTAA CGTGATGCGC 
TCCATCCATG GGGTGGGAGA GCTGATGACA CAGGTGGTGT TGAGGGGTGG GGGATTTCTG 
CCAGCCTCCA GCCACAATCC TCCTGACAGA GACGAAGTGA AAGCACAAAG TGAACCTCAG 
AAACAGGACA TACTGGTGAT GGACACTAAA CTCAAGATCA TCGAGATCCT GCAGTTTATT 
CTGAATGTGC GGTTGGACTA CAGGATCTCC TGCCTCCTCT GTATCTTTAA GACAGAGTTT 
GATGAAAGTA ACTCTCAAAG TGAACCATCT TTAAGCCCAG AATCTCCTGC CAGCGTTCAA 
GGTGCGCTAG ACTTTGAGCA CATTGAAGAG CAGGCGGAGG GTATTTTTGG AGGAAGTGAA 
GAGAACACCC CGCTGGATCT TGATGATCAT GGTGGACGGA CATTTCTGAG GGTCCTGCTT 
CACCTGACCA TGCATGACTA TCCTCCGCTG GTATCTCGAG CTCTTCACCT GCTCTTCAGA 
CACTTTAGCC AACGCCAGGA GGTCCTACAG GCCTTCAAAC AGGTTCAGCT CCTGGTGACC 
AGTCAGGATG TGGAAAACTA TAAACAGATC AAGTCAGATT TGGACCAGCT GAGGTCAATA 
GTAGAGAAAT CAGAGTTGTG GGTGTATAAG AGACAAGGCT CAGACTCAGG ACTGGATGCT 
GGAGAAGTCA CCCCTGAAGC CCACCACAAG GGTGTCATCA ACTCGAGTAG ATCAAACAAA 
CCCAAAGTGG AAAGTACCAG CAGCTCGAAC TACAGAGTCG TGAAAGAGAT CCTGCTGAGG 
CTGAGCAAAC TGTGTGTTTT GGAGGGCATC TCTGGCAAGA AGAATAAGAA ACAGCAGCAG 
CGGCTCCTCA GGAACATGGG AGCTCACAGT GTGGTCCTCG AACTGTTGCA AATCCCTTAT 
GAGAAGGGGG AGGATGTCCA GATGCAGGAG ATCATGACCT TAGCACATCA GTTCCTTCAA 
AACTTCTGTG CTGGAAATCA GCAGAACCAG GCACTTCTAC ACAAACACAT CAACCTGTTC 
CTCAACCCTG GGATCCTGGA GGCGGTGACC ATGCAGCATA TCTTCATGAA TAATTTCCAG 
TTGTGTAGTG AGATTAATGA GCGGGTCGTG CAGCATTTTG TGCACTGCAT CGAGACACAC 
GGCCGCAGCG TGCACTACCT CAAGTTCCTG CAGACCATTG TCAAGGCAGA GAACAAGTTC 
ATCAAGAAAT GCCAAGACAT CGTCATGGCT GAGCTGGTGA CTGCAGGTGA GGATGTGCTG 
GTGTTCTATA ATGACCGGGC GTCTTTTCAG TCATTGGTTC AGATGATGCG TCTTGAGCGG 
GAACGACTTG ATGAGAGCAG TGCACTCAGG TATCACATTC ACCTGGTGGA GTTGCTGGCT 
GTGTGCACTG AAGGCAAGAA CGTCTACACT GAGATCAAAT GCAACTCGCT GCTCCCTCTC 
GATGATATTG TACGTGTGGT CACACATGAG GACTGCATCC CTGAGGTGAA GATGGCCTAC 
GTGAACTTCC TGAATCACTG TTATGTTGAT ACAGAGGTTG AGATGAAGGA GATCTACACC 
AGCAATCACA TGTGGAAACT TTTTGATGAT TTTCTGGTGG ACGTCTGCAG AGTGTGCAAT 
AACACAAGTG ACCGTAAACA CGCTGACACA GTTCTGGAGA GATACGTTAC AGAGACCATA 
ATGAGCATCG TCACCACCTT TTTCAGCTCA CCATTCTCAG ACCAGAGCAC AAGTTTGCAG 
ACCCGGCAGC CGGTGTTTGT GCAGCTGCTG CAGGGAGTGT TTCGTGTATA TCACTGCAAC 
TGGCTTCTCC CAGGTCAGAA GGGGAATGTG GAAGCCTGCA TCAAAGTGCT GAGTGACGTG 
GCCAAAGGCA GAGCCATAGC CATTCCTGTG GATCTGGACT GTCAGGTGAA TAATCTGTTC 
ATGAAGTCCA ACAACATTGT GCAGAAAACA GCCCTCAGCT GGAGACTCTC TGTCCGTAAC 
GCGACTCGGA GAGACTCTGT TCTGACCACC TCTCGAGACT ATCGCAACAT CATTGAAAGA 
CTACAGGATA TAGTGTCTGC ATTGGAGGAG CGGTTGAGGC CTCTGGTTCA GGCTGAGTTG 
TCAGTGTTGG TGGATGTTCT CCATCGCCCA GAGCTGCTGT TTCCAGAGCA CACTGAAGCC 
CACCGCAAGT GTGAGAGTGG AGGCTTCATC TGCAAGTTGA TAAAACACAC TAAGCAGCTT 
CTAGAAGAAA ACGAAGAGAG GCTTTGCATC AAAGTCCTTC AGACACTCCG AGAGATGATG 
ACCAAAGACA GAGGATATGG AGAGAAGCTT CTGGGATATG ATGATGAGAT GGATGTCACG 
GAGGTGGTGG ATATCAGTCT GCCACCCAAA CAGCAGGAGG ATCGGCAGAG GGGGGAGGCC 
CTTCGGCAGC TGCTCGTCAA TCGTTACTAT GGCTTCAGGA GTGGTGGGCG GAGAGAAAGT 
CTGACCTCGT TTGGTAACTC CACCCTAACT CCTGTCGGGC CAATCAAGAG CCAGCCAGGG 
GGGTTGAGCA GGGCTGAGAT GTCATTAATG GAGGTGCAGT GTCATCTAGA CCGTGAAGGG 
GCATCGGATC TTGTCATTGA TCTCATCATG AACACTACCA GCGACCGCGT CTTCCATGAA 
AGCATTCTGT TGGCCATCGC ACTGTTGGAG GGCGGAAACA CCATCATACA GCATTCCTTC 
TTCAAACGTT TAACAGAAGA CAAAAATTCA GAGAAGTTTT TCAGGGTGTT TTATGACAGA 
ATGAAAGTGG CTCAGGTGGA GATCAAAGCC ACGGTTACTG TCAACACTAG TGACCTCGGC 
AACAAGCGGC GTGACGACAA CACTCCAGAC AAAGATACTC CACAACGCCG CAGAGGTAAA 
GACTCCGGTG TGGTGGTGAC AGATGATGCT CGTGAGCAGC TGTTGGAGGC TTCAGCTGCG 
ACTAAGAAGG CCTTTGGTTC ATATCGTCGA GATGCTGATC CCGAGGAGAC GTTTGGCACG 
GCTGATGGAG ATAAAGGAGG TGGAGATAAA GGCACAGAGC AGGGCGAGAT GAGCCCTGTC 
ATCCTCATAA TGCAGCCAAT CCTGCGCTTC CTGCAGCTGC TGTGCGAAAA CCACAATCGA 
GACCTGCAGA ATTTTCTGCG CTGTCAGAAC AATAAAAACA ACTATAATCT GGTGTGTGAG 
ACGCTGCAGT TCCTGGACTG TATCTGTGGC AGCACAACAG GAGGCCTGGG GCTGTTAGGA 
CTCTACATCA ACCAGCATAA CGTAGCGCTC ATTAATCAGA CTGTAGAGAG CCTCACTGAA 
TACTGTCAAG GTCCCTGCCA TGACAATCAG AATTGCATTG CCACCCATGA GTCTAATGGC 
ATCGACATCA TTATTGCACT GATTCTGAAT GATATTAATC CACTGGGCAG GAAGAGAATG 
GATCTGGTTC TAGAACTAAA GAATAATGCA TCTAAGCTGC TGCTGGCCAT CATGGAGAGC 
CGTCATGACA GTGAGAATGC AGAAAGAATT CTGTACAACA TGAGACCAAA AGAACTAGTG 
GAGGTGATGA AAATGGCCTA CCAGCAGGGG GAGGCAGAGT TTGAGGATGA GGAACAGGAG 
AATGGAGAGG ATCATGCAGC ATCGCCTCGC AATGTTGGAC ACAACATCTA TATTCTTGCA 
CATCAGCTGT CGCGTCACAA TAAGGAATTG CAAATACTAC TGAAACCAGG TGGAGAGGAC 
CAGGCCCTGG AGTATTACAC AAAACACACC TCACAGATAG AGATAGTTCG ACAGGATCGC 
ACTATGGAAC AGATTGTGTT TCCTGTACCA AATATCTGCT CTTTTCTGAC AAATGAGTCC 
AAGCTGCGTG TGTACTATGG TACAGAGAGA GACGAGCAAG GCAGTAAAAT AAATGACTTC 
TTCTTGCATG CAGACGACCT CTTTAATGAA ATGAGGTGGC AGAAAAAACT AAGAGCTCAG 
CCAGTGCTGT ACTGGTGCAG CAGGAACATG TCGTTTTGGA GTAACGTTTC CTTTAATCTG 
GCGGTGCTCA TTAATTTGCT GGTGGCATTT TTCTACCCTC TGGATGGAGT GAGCGAAAGC 
CAGCTGGAAC CTTCTCTGTC TCTGTTGCTG TGGGTTTGTT TGTTGGGCTC ACTGGGCTTC 
GTGTTGATGT CCCCGAGGCC AAATGCAGTG CGGGTGCTGG TCATCTCCAC TGTCCTGCAG 
TTGGGCTTCT CTGTAGGGCT GCAGCACATG CTCACACTCC TGGGAGCTTT CAATGTTTGT 
AATAAGATCG TGTTCATGCT GAGCTTTGTG GGTAACCGTG GGTCATTCAC CCGGGGTTAT 
CGGGTGATGG TCATGGATAG AGAGTTCCTG TTTCATCTGC TGTACCTTCT CATCTGCATA 
CTGGGCCTTT TTGGACACGT CTTCTTTTAC AGTCTTCTGT TGTTTGACCT GGTGAACCGG 
GAAGAGACTC TTCTGAACGT CATCAAGAGT GTGACTCGAA ACGGCCGCTC CATCGTCTTG 
ACCGCAGTGC TGGGCCTCAT CCTGGTCTAC CTCTTTTCTA TTGTTGGATA TATGTTTTTC 
AAAGATGACT TCATCCTGGA GGTGAACCGC ATCTCCAACG GCACTCTGGA GGAGGGGGTG 
ACTTTACCTG CTGGTTTTTG TGATGCGAGA AACGGGTCGT GTCTTTCTGA TGATGTTGAC 
GAGGATGACG TGGAGCGCGC GTGTGATTCT CTATGGATGT GTATGATCAC TGTACTCAGT 
CATGGCCTCA GGAGCGGTGG AGGAGTTGGA GATGTCCTGC GCAAACCGTC TAAAGAGGAG 
CGTCTGTTCG CCGCACGGGT GGTGTACGAT CTCCTGTTCT TCTTCCTGGT GATCATCATT 
GTGTTGAATC TGATCTTCGG TGTGATCATT GACACATTTG CAGATCTGAG AAGCGAGAAG 
CAGAGAAAAG AGGAGATCTT GAAGACCACA TGCTTCATCT GTGGTCTGGA GAGAGATAAG 
TTTGATAATA AAACGGTGAC GTTTGAAGAG CACATCAAGG AGGAGCACAA CCTGTGGCAT 
TACCTCTTCT TCATAGTGCT GGTGCGGGTC AAAGACTCCA CCGAATACAC GGGACCCGAG 
AGCTACGTCG CTCAGATGAT CAAGGAGCAC AATCTGGACT GGTTTCCTCG TATGAGGGCG 
ATGTCTCTGG TCAGCAGTGA TGGAGAAGGT GAACAGAATG AACTGCGCAG CTTACAGGAG 
AAACTCGAGT CCACAATGAG GCTCGTCTCT AATCTTACCA ATCAGCTCAC AGAGCTCAAA 
GAGCAGATGA CTGAGCAAAG GAAACACAAG CAGAGGCTCG GTCTGCTGGG AAACCCCGCC 
CACCTCAACA TCAACCCACA GCAGCCAGCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itpr1a ( XM_009302587.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300862.1 Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X3, mRNA.

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CloneID	ODa45194
Clone ID Related Accession (Same CDS sequence)	XM_009302587.3 , XM_009302587.2
Accession Version	XM_009302587.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009302587.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GATGGATTCG 
GAGCATGAAG TGATTCGGGC TCGTGCACGT AACCCTCAGG ATAAGGTGAT GTACACACTG 
GTGTCTGTTC CTGATGGAAA TGACATATCC TCCATCTTTG AGCTTGACCC TACAACTTTG 
AGGGGCGGAG ACTCACTTGT GCCAAGAAAC TCGTATGTGA GGCTCAGGCA TCTCTGCACC 
AACACGTGGG TCCACAGCAC CAACCAGCCA ATAGATAAAG AGGAAGAGAA ACCGGTCATG 
CTCAGAATAG GAACGTCTCC TCTGAAGGAA GATAAAGAGG CGTTTGCTAT AGTACCAGTC 
TCTCCAGCTG AGGTTCGAGA TCTGGATTTT GCTAATGATG CTAGTAAAGT GTTGGCTTCC 
ATTGCTGCCA AACTGGAGAA GGGCACCATT ACCCAGAATG AGAGGAGGTC AGTGACGAAG 
CTGTTGGAGG ATCTGGTGTA TTTTGTGGTG GACATACCCA GCAGTGCTCA AGATGTGCTG 
GAGATCACAG TCAACAAACC CAACAGAGAG CGACAGAAAC TCATGAGAGA GCAGAACATT 
CTTAAACAGA TCTTCAAGCT GCTGCAGGCG CCATTTACAG ACAGCGGGGA CGGCCCTATG 
TTGCGATTAG AAGAGCTCGC AGACCAGCGT CATGCACCCT TTCGACACAT TTGCAGACTC 
TGTTACAGAG TCCTACGTTA CTCACAACAG GACTATCGCA AAAATCAGGA GTACATAGCA 
AAGCAGTTCC GCTTCATGCA GAAGCAGATA GGATATGATG TGCTGGCTGA AGACACAATT 
ACAGCCCTTC TGCACAACAA CAGGAAGCTC CTAGAGAAAC ACATCACAGC AGCTGAAATA 
GATACCTTTG TCAGTCTGGT CCGCAAGAAC AGAGAGCCCA GATTTCTGGA TTACTTGTCA 
GATCTGTGTG TGTCGATGAA TAAATCCATC CCGGTCACTC AAGAGCTCAT CTGTAATGCT 
GTGCTGGACC CAGCCAATGC AGACATTCTC ATCGAAACCA AGCTTGTGTT GTCACGGTTT 
GAGGTGGCAG GAACGGTGTT GGGTGAAGGT GCGGAGGAGG AAGAGGAGGA TGAAGAGGAG 
GTCTGGCTGT TCTGGAAGGA CAGTGGAGGA GAGGTGAAGA GTAAAAGCAT ACGAGAGCTT 
GCACAGGACG CAAAAGATGG ACATACAGAG GACCAGGAAG TCATCAATTA TTACAGATAT 
CAGCTGAATT TGTTTGCTCG GATGTGTCTG GATCGACAGT ATCTTGCGAT TAACAAGATA 
TCAGCACAGC TGGATGTGGA TCTGATTCTG CGCTGCATGT CAGATGAAGA TTTGCCCTTT 
GACCTCAGAG CATCGTTCTG CCGCATGATG CTGCACATGC ATGTAGACCG TGACCCTCAG 
GAGCAGGTCA CCCCGGTCAA ATATGCTCGA CTGTGGTCTG AAATCCCATC ACAAATTTCC 
ATTGATGATT ATGATAATGA TGGGACGTCA AGTGATGAAG TGAAAGAGCG CTTCTCTCAG 
ACGATGGAGT TTGTGGAGAA TTACCTGAGG GATGTTGTCT GCCAGAGTTT CCCGTTCTCT 
GATAAGGAGA AAAACAAACT CACTTTTGAG GTGGTGAACC TGGCAAGAAA CCTGATCTAT 
TTTGGTTTCT ATAACTTCAG TGATCTGCTA AGGTTAACAA AAATCCTATT GGCAATCCTG 
GACTGTGTCC ACATAAGCAC TCCCTTCCAC ATCAAGCTGG ATAGAGAACC CGGTAAAGGA 
AGTAACGTGA TGCGCTCCAT CCATGGGGTG GGAGAGCTGA TGACACAGGT GGTGTTGAGG 
GGTGGGGGAT TTCTGCCAGC CTCCAGCCAC AATCCTCCTG ACAGAGACGA AGTGAAAGCA 
CAAAGTGAAC CTCAGAAACA GGACATACTG GTGATGGACA CTAAACTCAA GATCATCGAG 
ATCCTGCAGT TTATTCTGAA TGTGCGGTTG GACTACAGGA TCTCCTGCCT CCTCTGTATC 
TTTAAGACAG AGTTTGATGA AAGTAACTCT CAAAGTGAAC CATCTTTAAG CCCAGAATCT 
CCTGCCAGCG TTCAAGGTGC GCTAGACTTT GAGCACATTG AAGAGCAGGC GGAGGGTATT 
TTTGGAGGAA GTAGTGAAGA GAACACCCCG CTGGATCTTG ATGATCATGG TGGACGGACA 
TTTCTGAGGG TCCTGCTTCA CCTGACCATG CATGACTATC CTCCGCTGGT ATCTCGAGCT 
CTTCACCTGC TCTTCAGACA CTTTAGCCAA CGCCAGGAGG TCCTACAGGC CTTCAAACAG 
GTTCAGCTCC TGGTGACCAG TCAGGATGTG GAAAACTATA AACAGATCAA GTCAGATTTG 
GACCAGCTGA GGTCAATAGT AGAGAAATCA GAGTTGTGGG TGTATAAGAG ACAAGGCTCA 
GACTCAGGAC TGGATGCTGG AGAAGTCACC CCTGAAGCCC ACCACAAGGG TGTCATCAAC 
TCGAGTAGAT CAAACAAACC CAAAGTGGAA AGTACCAGCA GCTCGAACTA CAGAGTCGTG 
AAAGAGATCC TGCTGAGGCT GAGCAAACTG TGTGTTTTGG AGGGCATCTC TGGCAAGAAG 
AATAAGAAAC AGCAGCAGCG GCTCCTCAGG AACATGGGAG CTCACAGTGT GGTCCTCGAA 
CTGTTGCAAA TCCCTTATGA GAAGGGGGAG GATGTCCAGA TGCAGGAGAT CATGACCTTA 
GCACATCAGT TCCTTCAAAA CTTCTGTGCT GGAAATCAGC AGAACCAGGC ACTTCTACAC 
AAACACATCA ACCTGTTCCT CAACCCTGGG ATCCTGGAGG CGGTGACCAT GCAGCATATC 
TTCATGAATA ATTTCCAGTT GTGTAGTGAG ATTAATGAGC GGGTCGTGCA GCATTTTGTG 
CACTGCATCG AGACACACGG CCGCAGCGTG CACTACCTCA AGTTCCTGCA GACCATTGTC 
AAGGCAGAGA ACAAGTTCAT CAAGAAATGC CAAGACATCG TCATGGCTGA GCTGGTGACT 
GCAGGTGAGG ATGTGCTGGT GTTCTATAAT GACCGGGCGT CTTTTCAGTC ATTGGTTCAG 
ATGATGCGTC TTGAGCGGGA ACGACTTGAT GAGAGCAGTG CACTCAGGTA TCACATTCAC 
CTGGTGGAGT TGCTGGCTGT GTGCACTGAA GGCAAGAACG TCTACACTGA GATCAAATGC 
AACTCGCTGC TCCCTCTCGA TGATATTGTA CGTGTGGTCA CACATGAGGA CTGCATCCCT 
GAGGTGAAGA TGGCCTACGT GAACTTCCTG AATCACTGTT ATGTTGATAC AGAGGTTGAG 
ATGAAGGAGA TCTACACCAG CAATCACATG TGGAAACTTT TTGATGATTT TCTGGTGGAC 
GTCTGCAGAG TGTGCAATAA CACAAGTGAC CGTAAACACG CTGACACAGT TCTGGAGAGA 
TACGTTACAG AGACCATAAT GAGCATCGTC ACCACCTTTT TCAGCTCACC ATTCTCAGAC 
CAGAGCACAA GTTTGCAGAC CCGGCAGCCG GTGTTTGTGC AGCTGCTGCA GGGAGTGTTT 
CGTGTATATC ACTGCAACTG GCTTCTCCCA GGTCAGAAGG GGAATGTGGA AGCCTGCATC 
AAAGTGCTGA GTGACGTGGC CAAAGGCAGA GCCATAGCCA TTCCTGTGGA TCTGGACTGT 
CAGGTGAATA ATCTGTTCAT GAAGTCCAAC AACATTGTGC AGAAAACAGC CCTCAGCTGG 
AGACTCTCTG TCCGTAACGC GACTCGGAGA GACTCTGTTC TGACCACCTC TCGAGACTAT 
CGCAACATCA TTGAAAGACT ACAGGATATA GTGTCTGCAT TGGAGGAGCG GTTGAGGCCT 
CTGGTTCAGG CTGAGTTGTC AGTGTTGGTG GATGTTCTCC ATCGCCCAGA GCTGCTGTTT 
CCAGAGCACA CTGAAGCCCA CCGCAAGTGT GAGAGTGGAG GCTTCATCTG CAAGTTGATA 
AAACACACTA AGCAGCTTCT AGAAGAAAAC GAAGAGAGGC TTTGCATCAA AGTCCTTCAG 
ACACTCCGAG AGATGATGAC CAAAGACAGA GGATATGGAG AGAAGCTTCT GGGATATGAT 
GATGAGATGG ATGTCACGGA GGTGGTGGAT ATCAGTCTGC CACCCAAACA GCAGGAGGAT 
CGGCAGAGGG GGGAGGCCCT TCGGCAGCTG CTCGTCAATC GTTACTATGG CTTCAGGAGT 
GGTGGGCGGA GAGAAAGTCT GACCTCGTTT GGTAACTCCA CCCTAACTCC TGTCGGGCCA 
ATCAAGAGCC AGCCAGGGGG GTTGAGCAGG GCTGAGATGT CATTAATGGA GGTGCAGTGT 
CATCTAGACC GTGAAGGGGC ATCGGATCTT GTCATTGATC TCATCATGAA CACTACCAGC 
GACCGCGTCT TCCATGAAAG CATTCTGTTG GCCATCGCAC TGTTGGAGGG CGGAAACACC 
ATCATACAGC ATTCCTTCTT CAAACGTTTA ACAGAAGACA AAAATTCAGA GAAGTTTTTC 
AGGGTGTTTT ATGACAGAAT GAAAGTGGCT CAGGTGGAGA TCAAAGCCAC GGTTACTGTC 
AACACTAGTG ACCTCGGCAA CAAGCGGCGT GACGACAACA CTCCAGACAA AGATACTCCA 
CAACGCCGCA GAGGTAAAGA CTCCGGTGTG GTGGTGACAG ATGATGCTCG TGAGCAGCTG 
TTGGAGGCTT CAGCTGCGAC TAAGAAGGCC TTTGGTTCAT ATCGTCGAGA TGCTGATCCC 
GAGGAGACGT TTGGCACGGC TGATGGAGAT AAAGGAGGTG GAGATAAAGG CACAGAGCAG 
GGCGAGATGA GCCCTGTCAT CCTCATAATG CAGCCAATCC TGCGCTTCCT GCAGCTGCTG 
TGCGAAAACC ACAATCGAGA CCTGCAGAAT TTTCTGCGCT GTCAGAACAA TAAAAACAAC 
TATAATCTGG TGTGTGAGAC GCTGCAGTTC CTGGACTGTA TCTGTGGCAG CACAACAGGA 
GGCCTGGGGC TGTTAGGACT CTACATCAAC CAGCATAACG TAGCGCTCAT TAATCAGACT 
GTAGAGAGCC TCACTGAATA CTGTCAAGGT CCCTGCCATG ACAATCAGAA TTGCATTGCC 
ACCCATGAGT CTAATGGCAT CGACATCATT ATTGCACTGA TTCTGAATGA TATTAATCCA 
CTGGGCAGGA AGAGAATGGA TCTGGTTCTA GAACTAAAGA ATAATGCATC TAAGCTGCTG 
CTGGCCATCA TGGAGAGCCG TCATGACAGT GAGAATGCAG AAAGAATTCT GTACAACATG 
AGACCAAAAG AACTAGTGGA GGTGATGAAA ATGGCCTACC AGCAGGGGGA GGCAGAGTTT 
GAGGATGAGG AACAGGAGAA TGGAGAGGAT CATGCAGCAT CGCCTCGCAA TGTTGGACAC 
AACATCTATA TTCTTGCACA TCAGCTGTCG CGTCACAATA AGGAATTGCA AATACTACTG 
AAACCAGGTG GAGAGGACCA GGCCCTGGAG TATTACACAA AACACACCTC ACAGATAGAG 
ATAGTTCGAC AGGATCGCAC TATGGAACAG ATTGTGTTTC CTGTACCAAA TATCTGCTCT 
TTTCTGACAA ATGAGTCCAA GCTGCGTGTG TACTATGGTA CAGAGAGAGA CGAGCAAGGC 
AGTAAAATAA ATGACTTCTT CTTGCATGCA GACGACCTCT TTAATGAAAT GAGGTGGCAG 
AAAAAACTAA GAGCTCAGCC AGTGCTGTAC TGGTGCAGCA GGAACATGTC GTTTTGGAGT 
AACGTTTCCT TTAATCTGGC GGTGCTCATT AATTTGCTGG TGGCATTTTT CTACCCTCTG 
GATGGAGTGA GCGAAAGCCA GCTGGAACCT TCTCTGTCTC TGTTGCTGTG GGTTTGTTTG 
TTGGGCTCAC TGGGCTTCGT GTTGATGTCC CCGAGGCCAA ATGCAGTGCG GGTGCTGGTC 
ATCTCCACTG TCCTGCAGTT GGGCTTCTCT GTAGGGCTGC AGCACATGCT CACACTCCTG 
GGAGCTTTCA ATGTTTGTAA TAAGATCGTG TTCATGCTGA GCTTTGTGGG TAACCGTGGG 
TCATTCACCC GGGGTTATCG GGTGATGGTC ATGGATAGAG AGTTCCTGTT TCATCTGCTG 
TACCTTCTCA TCTGCATACT GGGCCTTTTT GGACACGTCT TCTTTTACAG TCTTCTGTTG 
TTTGACCTGG TGAACCGGGA AGAGACTCTT CTGAACGTCA TCAAGAGTGT GACTCGAAAC 
GGCCGCTCCA TCGTCTTGAC CGCAGTGCTG GGCCTCATCC TGGTCTACCT CTTTTCTATT 
GTTGGATATA TGTTTTTCAA AGATGACTTC ATCCTGGAGG TGAACCGCAT CTCCAACGGC 
ACTCTGGAGG AGGGGGTGAC TTTACCTGCT GGTTTTTGTG ATGCGAGAAA CGGGTCGTGT 
CTTTCTGATG ATGTTGACGA GGATGACGTG GAGCGCGCGT GTGATTCTCT ATGGATGTGT 
ATGATCACTG TACTCAGTCA TGGCCTCAGG AGCGGTGGAG GAGTTGGAGA TGTCCTGCGC 
AAACCGTCTA AAGAGGAGCG TCTGTTCGCC GCACGGGTGG TGTACGATCT CCTGTTCTTC 
TTCCTGGTGA TCATCATTGT GTTGAATCTG ATCTTCGGTG TGATCATTGA CACATTTGCA 
GATCTGAGAA GCGAGAAGCA GAGAAAAGAG GAGATCTTGA AGACCACATG CTTCATCTGT 
GGTCTGGAGA GAGATAAGTT TGATAATAAA ACGGTGACGT TTGAAGAGCA CATCAAGGAG 
GAGCACAACC TGTGGCATTA CCTCTTCTTC ATAGTGCTGG TGCGGGTCAA AGACTCCACC 
GAATACACGG GACCCGAGAG CTACGTCGCT CAGATGATCA AGGAGCACAA TCTGGACTGG 
TTTCCTCGTA TGAGGGCGAT GTCTCTGGTC AGCAGTGATG GAGAAGGTGA ACAGAATGAA 
CTGCGCAGCT TACAGGAGAA ACTCGAGTCC ACAATGAGGC TCGTCTCTAA TCTTACCAAT 
CAGCTCACAG AGCTCAAAGA GCAGATGACT GAGCAAAGGA AACACAAGCA GAGGCTCGGT 
CTGCTGGGAA ACCCCGCCCA CCTCAACATC AACCCACAGC AGCCAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300862.1
CDS	148..8238
Translation	MVDKLSSFLHIGDVCSLYAEGSTSGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGTSTTDTVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDAAGNEGSWFYIQPFYKLRSIGDNVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCTTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDEHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEMDSEHEVIRARARNPQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSPLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAAKLEKGTITQNERRSVTKLLEDLVYFVVDIPSSAQDVLEITVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRYSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDPANADILIETKLVLSRFEVAGTVLGEGAEEEEEDEEEVWLFWKDSGGEVKSKSIRELAQDAKDGHTEDQEVINYYRYQLNLFARMCLDRQYLAINKISAQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSQISIDDYDNDGTSSDEVKERFSQTMEFVENYLRDVVCQSFPFSDKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHISTPFHIKLDREPGKGSNVMRSIHGVGELMTQVVLRGGGFLPASSHNPPDRDEVKAQSEPQKQDILVMDTKLKIIEILQFILNVRLDYRISCLLCIFKTEFDESNSQSEPSLSPESPASVQGALDFEHIEEQAEGIFGGSSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALHLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSDSGLDAGEVTPEAHHKGVINSSRSNKPKVESTSSSNYRVVKEILLRLSKLCVLEGISGKKNKKQQQRLLRNMGAHSVVLELLQIPYEKGEDVQMQEIMTLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAVTMQHIFMNNFQLCSEINERVVQHFVHCIETHGRSVHYLKFLQTIVKAENKFIKKCQDIVMAELVTAGEDVLVFYNDRASFQSLVQMMRLERERLDESSALRYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKMAYVNFLNHCYVDTEVEMKEIYTSNHMWKLFDDFLVDVCRVCNNTSDRKHADTVLERYVTETIMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQGVFRVYHCNWLLPGQKGNVEACIKVLSDVAKGRAIAIPVDLDCQVNNLFMKSNNIVQKTALSWRLSVRNATRRDSVLTTSRDYRNIIERLQDIVSALEERLRPLVQAELSVLVDVLHRPELLFPEHTEAHRKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKLLGYDDEMDVTEVVDISLPPKQQEDRQRGEALRQLLVNRYYGFRSGGRRESLTSFGNSTLTPVGPIKSQPGGLSRAEMSLMEVQCHLDREGASDLVIDLIMNTTSDRVFHESILLAIALLEGGNTIIQHSFFKRLTEDKNSEKFFRVFYDRMKVAQVEIKATVTVNTSDLGNKRRDDNTPDKDTPQRRRGKDSGVVVTDDAREQLLEASAATKKAFGSYRRDADPEETFGTADGDKGGGDKGTEQGEMSPVILIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINQHNVALINQTVESLTEYCQGPCHDNQNCIATHESNGIDIIIALILNDINPLGRKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVMKMAYQQGEAEFEDEEQENGEDHAASPRNVGHNIYILAHQLSRHNKELQILLKPGGEDQALEYYTKHTSQIEIVRQDRTMEQIVFPVPNICSFLTNESKLRVYYGTERDEQGSKINDFFLHADDLFNEMRWQKKLRAQPVLYWCSRNMSFWSNVSFNLAVLINLLVAFFYPLDGVSESQLEPSLSLLLWVCLLGSLGFVLMSPRPNAVRVLVISTVLQLGFSVGLQHMLTLLGAFNVCNKIVFMLSFVGNRGSFTRGYRVMVMDREFLFHLLYLLICILGLFGHVFFYSLLLFDLVNREETLLNVIKSVTRNGRSIVLTAVLGLILVYLFSIVGYMFFKDDFILEVNRISNGTLEEGVTLPAGFCDARNGSCLSDDVDEDDVERACDSLWMCMITVLSHGLRSGGGVGDVLRKPSKEERLFAARVVYDLLFFFLVIIIVLNLIFGVIIDTFADLRSEKQRKEEILKTTCFICGLERDKFDNKTVTFEEHIKEEHNLWHYLFFIVLVRVKDSTEYTGPESYVAQMIKEHNLDWFPRMRAMSLVSSDGEGEQNELRSLQEKLESTMRLVSNLTNQLTELKEQMTEQRKHKQRLGLLGNPAHLNINPQQPA

Target ORF information:

RefSeq Version	XM_009302587.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302587.3

ORF Insert Sequence:

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GATGGATTCG 
GAGCATGAAG TGATTCGGGC TCGTGCACGT AACCCTCAGG ATAAGGTGAT GTACACACTG 
GTGTCTGTTC CTGATGGAAA TGACATATCC TCCATCTTTG AGCTTGACCC TACAACTTTG 
AGGGGCGGAG ACTCACTTGT GCCAAGAAAC TCGTATGTGA GGCTCAGGCA TCTCTGCACC 
AACACGTGGG TCCACAGCAC CAACCAGCCA ATAGATAAAG AGGAAGAGAA ACCGGTCATG 
CTCAGAATAG GAACGTCTCC TCTGAAGGAA GATAAAGAGG CGTTTGCTAT AGTACCAGTC 
TCTCCAGCTG AGGTTCGAGA TCTGGATTTT GCTAATGATG CTAGTAAAGT GTTGGCTTCC 
ATTGCTGCCA AACTGGAGAA GGGCACCATT ACCCAGAATG AGAGGAGGTC AGTGACGAAG 
CTGTTGGAGG ATCTGGTGTA TTTTGTGGTG GACATACCCA GCAGTGCTCA AGATGTGCTG 
GAGATCACAG TCAACAAACC CAACAGAGAG CGACAGAAAC TCATGAGAGA GCAGAACATT 
CTTAAACAGA TCTTCAAGCT GCTGCAGGCG CCATTTACAG ACAGCGGGGA CGGCCCTATG 
TTGCGATTAG AAGAGCTCGC AGACCAGCGT CATGCACCCT TTCGACACAT TTGCAGACTC 
TGTTACAGAG TCCTACGTTA CTCACAACAG GACTATCGCA AAAATCAGGA GTACATAGCA 
AAGCAGTTCC GCTTCATGCA GAAGCAGATA GGATATGATG TGCTGGCTGA AGACACAATT 
ACAGCCCTTC TGCACAACAA CAGGAAGCTC CTAGAGAAAC ACATCACAGC AGCTGAAATA 
GATACCTTTG TCAGTCTGGT CCGCAAGAAC AGAGAGCCCA GATTTCTGGA TTACTTGTCA 
GATCTGTGTG TGTCGATGAA TAAATCCATC CCGGTCACTC AAGAGCTCAT CTGTAATGCT 
GTGCTGGACC CAGCCAATGC AGACATTCTC ATCGAAACCA AGCTTGTGTT GTCACGGTTT 
GAGGTGGCAG GAACGGTGTT GGGTGAAGGT GCGGAGGAGG AAGAGGAGGA TGAAGAGGAG 
GTCTGGCTGT TCTGGAAGGA CAGTGGAGGA GAGGTGAAGA GTAAAAGCAT ACGAGAGCTT 
GCACAGGACG CAAAAGATGG ACATACAGAG GACCAGGAAG TCATCAATTA TTACAGATAT 
CAGCTGAATT TGTTTGCTCG GATGTGTCTG GATCGACAGT ATCTTGCGAT TAACAAGATA 
TCAGCACAGC TGGATGTGGA TCTGATTCTG CGCTGCATGT CAGATGAAGA TTTGCCCTTT 
GACCTCAGAG CATCGTTCTG CCGCATGATG CTGCACATGC ATGTAGACCG TGACCCTCAG 
GAGCAGGTCA CCCCGGTCAA ATATGCTCGA CTGTGGTCTG AAATCCCATC ACAAATTTCC 
ATTGATGATT ATGATAATGA TGGGACGTCA AGTGATGAAG TGAAAGAGCG CTTCTCTCAG 
ACGATGGAGT TTGTGGAGAA TTACCTGAGG GATGTTGTCT GCCAGAGTTT CCCGTTCTCT 
GATAAGGAGA AAAACAAACT CACTTTTGAG GTGGTGAACC TGGCAAGAAA CCTGATCTAT 
TTTGGTTTCT ATAACTTCAG TGATCTGCTA AGGTTAACAA AAATCCTATT GGCAATCCTG 
GACTGTGTCC ACATAAGCAC TCCCTTCCAC ATCAAGCTGG ATAGAGAACC CGGTAAAGGA 
AGTAACGTGA TGCGCTCCAT CCATGGGGTG GGAGAGCTGA TGACACAGGT GGTGTTGAGG 
GGTGGGGGAT TTCTGCCAGC CTCCAGCCAC AATCCTCCTG ACAGAGACGA AGTGAAAGCA 
CAAAGTGAAC CTCAGAAACA GGACATACTG GTGATGGACA CTAAACTCAA GATCATCGAG 
ATCCTGCAGT TTATTCTGAA TGTGCGGTTG GACTACAGGA TCTCCTGCCT CCTCTGTATC 
TTTAAGACAG AGTTTGATGA AAGTAACTCT CAAAGTGAAC CATCTTTAAG CCCAGAATCT 
CCTGCCAGCG TTCAAGGTGC GCTAGACTTT GAGCACATTG AAGAGCAGGC GGAGGGTATT 
TTTGGAGGAA GTAGTGAAGA GAACACCCCG CTGGATCTTG ATGATCATGG TGGACGGACA 
TTTCTGAGGG TCCTGCTTCA CCTGACCATG CATGACTATC CTCCGCTGGT ATCTCGAGCT 
CTTCACCTGC TCTTCAGACA CTTTAGCCAA CGCCAGGAGG TCCTACAGGC CTTCAAACAG 
GTTCAGCTCC TGGTGACCAG TCAGGATGTG GAAAACTATA AACAGATCAA GTCAGATTTG 
GACCAGCTGA GGTCAATAGT AGAGAAATCA GAGTTGTGGG TGTATAAGAG ACAAGGCTCA 
GACTCAGGAC TGGATGCTGG AGAAGTCACC CCTGAAGCCC ACCACAAGGG TGTCATCAAC 
TCGAGTAGAT CAAACAAACC CAAAGTGGAA AGTACCAGCA GCTCGAACTA CAGAGTCGTG 
AAAGAGATCC TGCTGAGGCT GAGCAAACTG TGTGTTTTGG AGGGCATCTC TGGCAAGAAG 
AATAAGAAAC AGCAGCAGCG GCTCCTCAGG AACATGGGAG CTCACAGTGT GGTCCTCGAA 
CTGTTGCAAA TCCCTTATGA GAAGGGGGAG GATGTCCAGA TGCAGGAGAT CATGACCTTA 
GCACATCAGT TCCTTCAAAA CTTCTGTGCT GGAAATCAGC AGAACCAGGC ACTTCTACAC 
AAACACATCA ACCTGTTCCT CAACCCTGGG ATCCTGGAGG CGGTGACCAT GCAGCATATC 
TTCATGAATA ATTTCCAGTT GTGTAGTGAG ATTAATGAGC GGGTCGTGCA GCATTTTGTG 
CACTGCATCG AGACACACGG CCGCAGCGTG CACTACCTCA AGTTCCTGCA GACCATTGTC 
AAGGCAGAGA ACAAGTTCAT CAAGAAATGC CAAGACATCG TCATGGCTGA GCTGGTGACT 
GCAGGTGAGG ATGTGCTGGT GTTCTATAAT GACCGGGCGT CTTTTCAGTC ATTGGTTCAG 
ATGATGCGTC TTGAGCGGGA ACGACTTGAT GAGAGCAGTG CACTCAGGTA TCACATTCAC 
CTGGTGGAGT TGCTGGCTGT GTGCACTGAA GGCAAGAACG TCTACACTGA GATCAAATGC 
AACTCGCTGC TCCCTCTCGA TGATATTGTA CGTGTGGTCA CACATGAGGA CTGCATCCCT 
GAGGTGAAGA TGGCCTACGT GAACTTCCTG AATCACTGTT ATGTTGATAC AGAGGTTGAG 
ATGAAGGAGA TCTACACCAG CAATCACATG TGGAAACTTT TTGATGATTT TCTGGTGGAC 
GTCTGCAGAG TGTGCAATAA CACAAGTGAC CGTAAACACG CTGACACAGT TCTGGAGAGA 
TACGTTACAG AGACCATAAT GAGCATCGTC ACCACCTTTT TCAGCTCACC ATTCTCAGAC 
CAGAGCACAA GTTTGCAGAC CCGGCAGCCG GTGTTTGTGC AGCTGCTGCA GGGAGTGTTT 
CGTGTATATC ACTGCAACTG GCTTCTCCCA GGTCAGAAGG GGAATGTGGA AGCCTGCATC 
AAAGTGCTGA GTGACGTGGC CAAAGGCAGA GCCATAGCCA TTCCTGTGGA TCTGGACTGT 
CAGGTGAATA ATCTGTTCAT GAAGTCCAAC AACATTGTGC AGAAAACAGC CCTCAGCTGG 
AGACTCTCTG TCCGTAACGC GACTCGGAGA GACTCTGTTC TGACCACCTC TCGAGACTAT 
CGCAACATCA TTGAAAGACT ACAGGATATA GTGTCTGCAT TGGAGGAGCG GTTGAGGCCT 
CTGGTTCAGG CTGAGTTGTC AGTGTTGGTG GATGTTCTCC ATCGCCCAGA GCTGCTGTTT 
CCAGAGCACA CTGAAGCCCA CCGCAAGTGT GAGAGTGGAG GCTTCATCTG CAAGTTGATA 
AAACACACTA AGCAGCTTCT AGAAGAAAAC GAAGAGAGGC TTTGCATCAA AGTCCTTCAG 
ACACTCCGAG AGATGATGAC CAAAGACAGA GGATATGGAG AGAAGCTTCT GGGATATGAT 
GATGAGATGG ATGTCACGGA GGTGGTGGAT ATCAGTCTGC CACCCAAACA GCAGGAGGAT 
CGGCAGAGGG GGGAGGCCCT TCGGCAGCTG CTCGTCAATC GTTACTATGG CTTCAGGAGT 
GGTGGGCGGA GAGAAAGTCT GACCTCGTTT GGTAACTCCA CCCTAACTCC TGTCGGGCCA 
ATCAAGAGCC AGCCAGGGGG GTTGAGCAGG GCTGAGATGT CATTAATGGA GGTGCAGTGT 
CATCTAGACC GTGAAGGGGC ATCGGATCTT GTCATTGATC TCATCATGAA CACTACCAGC 
GACCGCGTCT TCCATGAAAG CATTCTGTTG GCCATCGCAC TGTTGGAGGG CGGAAACACC 
ATCATACAGC ATTCCTTCTT CAAACGTTTA ACAGAAGACA AAAATTCAGA GAAGTTTTTC 
AGGGTGTTTT ATGACAGAAT GAAAGTGGCT CAGGTGGAGA TCAAAGCCAC GGTTACTGTC 
AACACTAGTG ACCTCGGCAA CAAGCGGCGT GACGACAACA CTCCAGACAA AGATACTCCA 
CAACGCCGCA GAGGTAAAGA CTCCGGTGTG GTGGTGACAG ATGATGCTCG TGAGCAGCTG 
TTGGAGGCTT CAGCTGCGAC TAAGAAGGCC TTTGGTTCAT ATCGTCGAGA TGCTGATCCC 
GAGGAGACGT TTGGCACGGC TGATGGAGAT AAAGGAGGTG GAGATAAAGG CACAGAGCAG 
GGCGAGATGA GCCCTGTCAT CCTCATAATG CAGCCAATCC TGCGCTTCCT GCAGCTGCTG 
TGCGAAAACC ACAATCGAGA CCTGCAGAAT TTTCTGCGCT GTCAGAACAA TAAAAACAAC 
TATAATCTGG TGTGTGAGAC GCTGCAGTTC CTGGACTGTA TCTGTGGCAG CACAACAGGA 
GGCCTGGGGC TGTTAGGACT CTACATCAAC CAGCATAACG TAGCGCTCAT TAATCAGACT 
GTAGAGAGCC TCACTGAATA CTGTCAAGGT CCCTGCCATG ACAATCAGAA TTGCATTGCC 
ACCCATGAGT CTAATGGCAT CGACATCATT ATTGCACTGA TTCTGAATGA TATTAATCCA 
CTGGGCAGGA AGAGAATGGA TCTGGTTCTA GAACTAAAGA ATAATGCATC TAAGCTGCTG 
CTGGCCATCA TGGAGAGCCG TCATGACAGT GAGAATGCAG AAAGAATTCT GTACAACATG 
AGACCAAAAG AACTAGTGGA GGTGATGAAA ATGGCCTACC AGCAGGGGGA GGCAGAGTTT 
GAGGATGAGG AACAGGAGAA TGGAGAGGAT CATGCAGCAT CGCCTCGCAA TGTTGGACAC 
AACATCTATA TTCTTGCACA TCAGCTGTCG CGTCACAATA AGGAATTGCA AATACTACTG 
AAACCAGGTG GAGAGGACCA GGCCCTGGAG TATTACACAA AACACACCTC ACAGATAGAG 
ATAGTTCGAC AGGATCGCAC TATGGAACAG ATTGTGTTTC CTGTACCAAA TATCTGCTCT 
TTTCTGACAA ATGAGTCCAA GCTGCGTGTG TACTATGGTA CAGAGAGAGA CGAGCAAGGC 
AGTAAAATAA ATGACTTCTT CTTGCATGCA GACGACCTCT TTAATGAAAT GAGGTGGCAG 
AAAAAACTAA GAGCTCAGCC AGTGCTGTAC TGGTGCAGCA GGAACATGTC GTTTTGGAGT 
AACGTTTCCT TTAATCTGGC GGTGCTCATT AATTTGCTGG TGGCATTTTT CTACCCTCTG 
GATGGAGTGA GCGAAAGCCA GCTGGAACCT TCTCTGTCTC TGTTGCTGTG GGTTTGTTTG 
TTGGGCTCAC TGGGCTTCGT GTTGATGTCC CCGAGGCCAA ATGCAGTGCG GGTGCTGGTC 
ATCTCCACTG TCCTGCAGTT GGGCTTCTCT GTAGGGCTGC AGCACATGCT CACACTCCTG 
GGAGCTTTCA ATGTTTGTAA TAAGATCGTG TTCATGCTGA GCTTTGTGGG TAACCGTGGG 
TCATTCACCC GGGGTTATCG GGTGATGGTC ATGGATAGAG AGTTCCTGTT TCATCTGCTG 
TACCTTCTCA TCTGCATACT GGGCCTTTTT GGACACGTCT TCTTTTACAG TCTTCTGTTG 
TTTGACCTGG TGAACCGGGA AGAGACTCTT CTGAACGTCA TCAAGAGTGT GACTCGAAAC 
GGCCGCTCCA TCGTCTTGAC CGCAGTGCTG GGCCTCATCC TGGTCTACCT CTTTTCTATT 
GTTGGATATA TGTTTTTCAA AGATGACTTC ATCCTGGAGG TGAACCGCAT CTCCAACGGC 
ACTCTGGAGG AGGGGGTGAC TTTACCTGCT GGTTTTTGTG ATGCGAGAAA CGGGTCGTGT 
CTTTCTGATG ATGTTGACGA GGATGACGTG GAGCGCGCGT GTGATTCTCT ATGGATGTGT 
ATGATCACTG TACTCAGTCA TGGCCTCAGG AGCGGTGGAG GAGTTGGAGA TGTCCTGCGC 
AAACCGTCTA AAGAGGAGCG TCTGTTCGCC GCACGGGTGG TGTACGATCT CCTGTTCTTC 
TTCCTGGTGA TCATCATTGT GTTGAATCTG ATCTTCGGTG TGATCATTGA CACATTTGCA 
GATCTGAGAA GCGAGAAGCA GAGAAAAGAG GAGATCTTGA AGACCACATG CTTCATCTGT 
GGTCTGGAGA GAGATAAGTT TGATAATAAA ACGGTGACGT TTGAAGAGCA CATCAAGGAG 
GAGCACAACC TGTGGCATTA CCTCTTCTTC ATAGTGCTGG TGCGGGTCAA AGACTCCACC 
GAATACACGG GACCCGAGAG CTACGTCGCT CAGATGATCA AGGAGCACAA TCTGGACTGG 
TTTCCTCGTA TGAGGGCGAT GTCTCTGGTC AGCAGTGATG GAGAAGGTGA ACAGAATGAA 
CTGCGCAGCT TACAGGAGAA ACTCGAGTCC ACAATGAGGC TCGTCTCTAA TCTTACCAAT 
CAGCTCACAG AGCTCAAAGA GCAGATGACT GAGCAAAGGA AACACAAGCA GAGGCTCGGT 
CTGCTGGGAA ACCCCGCCCA CCTCAACATC AACCCACAGC AGCCAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itpr1a ( XM_009302587.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300862.1 Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X3, mRNA.

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CloneID	ODa45194
Clone ID Related Accession (Same CDS sequence)	XM_009302587.3 , XM_009302587.2
Accession Version	XM_009302587.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8091bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302587.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GATGGATTCG 
GAGCATGAAG TGATTCGGGC TCGTGCACGT AACCCTCAGG ATAAGGTGAT GTACACACTG 
GTGTCTGTTC CTGATGGAAA TGACATATCC TCCATCTTTG AGCTTGACCC TACAACTTTG 
AGGGGCGGAG ACTCACTTGT GCCAAGAAAC TCGTATGTGA GGCTCAGGCA TCTCTGCACC 
AACACGTGGG TCCACAGCAC CAACCAGCCA ATAGATAAAG AGGAAGAGAA ACCGGTCATG 
CTCAGAATAG GAACGTCTCC TCTGAAGGAA GATAAAGAGG CGTTTGCTAT AGTACCAGTC 
TCTCCAGCTG AGGTTCGAGA TCTGGATTTT GCTAATGATG CTAGTAAAGT GTTGGCTTCC 
ATTGCTGCCA AACTGGAGAA GGGCACCATT ACCCAGAATG AGAGGAGGTC AGTGACGAAG 
CTGTTGGAGG ATCTGGTGTA TTTTGTGGTG GACATACCCA GCAGTGCTCA AGATGTGCTG 
GAGATCACAG TCAACAAACC CAACAGAGAG CGACAGAAAC TCATGAGAGA GCAGAACATT 
CTTAAACAGA TCTTCAAGCT GCTGCAGGCG CCATTTACAG ACAGCGGGGA CGGCCCTATG 
TTGCGATTAG AAGAGCTCGC AGACCAGCGT CATGCACCCT TTCGACACAT TTGCAGACTC 
TGTTACAGAG TCCTACGTTA CTCACAACAG GACTATCGCA AAAATCAGGA GTACATAGCA 
AAGCAGTTCC GCTTCATGCA GAAGCAGATA GGATATGATG TGCTGGCTGA AGACACAATT 
ACAGCCCTTC TGCACAACAA CAGGAAGCTC CTAGAGAAAC ACATCACAGC AGCTGAAATA 
GATACCTTTG TCAGTCTGGT CCGCAAGAAC AGAGAGCCCA GATTTCTGGA TTACTTGTCA 
GATCTGTGTG TGTCGATGAA TAAATCCATC CCGGTCACTC AAGAGCTCAT CTGTAATGCT 
GTGCTGGACC CAGCCAATGC AGACATTCTC ATCGAAACCA AGCTTGTGTT GTCACGGTTT 
GAGGTGGCAG GAACGGTGTT GGGTGAAGGT GCGGAGGAGG AAGAGGAGGA TGAAGAGGAG 
GTCTGGCTGT TCTGGAAGGA CAGTGGAGGA GAGGTGAAGA GTAAAAGCAT ACGAGAGCTT 
GCACAGGACG CAAAAGATGG ACATACAGAG GACCAGGAAG TCATCAATTA TTACAGATAT 
CAGCTGAATT TGTTTGCTCG GATGTGTCTG GATCGACAGT ATCTTGCGAT TAACAAGATA 
TCAGCACAGC TGGATGTGGA TCTGATTCTG CGCTGCATGT CAGATGAAGA TTTGCCCTTT 
GACCTCAGAG CATCGTTCTG CCGCATGATG CTGCACATGC ATGTAGACCG TGACCCTCAG 
GAGCAGGTCA CCCCGGTCAA ATATGCTCGA CTGTGGTCTG AAATCCCATC ACAAATTTCC 
ATTGATGATT ATGATAATGA TGGGACGTCA AGTGATGAAG TGAAAGAGCG CTTCTCTCAG 
ACGATGGAGT TTGTGGAGAA TTACCTGAGG GATGTTGTCT GCCAGAGTTT CCCGTTCTCT 
GATAAGGAGA AAAACAAACT CACTTTTGAG GTGGTGAACC TGGCAAGAAA CCTGATCTAT 
TTTGGTTTCT ATAACTTCAG TGATCTGCTA AGGTTAACAA AAATCCTATT GGCAATCCTG 
GACTGTGTCC ACATAAGCAC TCCCTTCCAC ATCAAGCTGG ATAGAGAACC CGGTAAAGGA 
AGTAACGTGA TGCGCTCCAT CCATGGGGTG GGAGAGCTGA TGACACAGGT GGTGTTGAGG 
GGTGGGGGAT TTCTGCCAGC CTCCAGCCAC AATCCTCCTG ACAGAGACGA AGTGAAAGCA 
CAAAGTGAAC CTCAGAAACA GGACATACTG GTGATGGACA CTAAACTCAA GATCATCGAG 
ATCCTGCAGT TTATTCTGAA TGTGCGGTTG GACTACAGGA TCTCCTGCCT CCTCTGTATC 
TTTAAGACAG AGTTTGATGA AAGTAACTCT CAAAGTGAAC CATCTTTAAG CCCAGAATCT 
CCTGCCAGCG TTCAAGGTGC GCTAGACTTT GAGCACATTG AAGAGCAGGC GGAGGGTATT 
TTTGGAGGAA GTAGTGAAGA GAACACCCCG CTGGATCTTG ATGATCATGG TGGACGGACA 
TTTCTGAGGG TCCTGCTTCA CCTGACCATG CATGACTATC CTCCGCTGGT ATCTCGAGCT 
CTTCACCTGC TCTTCAGACA CTTTAGCCAA CGCCAGGAGG TCCTACAGGC CTTCAAACAG 
GTTCAGCTCC TGGTGACCAG TCAGGATGTG GAAAACTATA AACAGATCAA GTCAGATTTG 
GACCAGCTGA GGTCAATAGT AGAGAAATCA GAGTTGTGGG TGTATAAGAG ACAAGGCTCA 
GACTCAGGAC TGGATGCTGG AGAAGTCACC CCTGAAGCCC ACCACAAGGG TGTCATCAAC 
TCGAGTAGAT CAAACAAACC CAAAGTGGAA AGTACCAGCA GCTCGAACTA CAGAGTCGTG 
AAAGAGATCC TGCTGAGGCT GAGCAAACTG TGTGTTTTGG AGGGCATCTC TGGCAAGAAG 
AATAAGAAAC AGCAGCAGCG GCTCCTCAGG AACATGGGAG CTCACAGTGT GGTCCTCGAA 
CTGTTGCAAA TCCCTTATGA GAAGGGGGAG GATGTCCAGA TGCAGGAGAT CATGACCTTA 
GCACATCAGT TCCTTCAAAA CTTCTGTGCT GGAAATCAGC AGAACCAGGC ACTTCTACAC 
AAACACATCA ACCTGTTCCT CAACCCTGGG ATCCTGGAGG CGGTGACCAT GCAGCATATC 
TTCATGAATA ATTTCCAGTT GTGTAGTGAG ATTAATGAGC GGGTCGTGCA GCATTTTGTG 
CACTGCATCG AGACACACGG CCGCAGCGTG CACTACCTCA AGTTCCTGCA GACCATTGTC 
AAGGCAGAGA ACAAGTTCAT CAAGAAATGC CAAGACATCG TCATGGCTGA GCTGGTGACT 
GCAGGTGAGG ATGTGCTGGT GTTCTATAAT GACCGGGCGT CTTTTCAGTC ATTGGTTCAG 
ATGATGCGTC TTGAGCGGGA ACGACTTGAT GAGAGCAGTG CACTCAGGTA TCACATTCAC 
CTGGTGGAGT TGCTGGCTGT GTGCACTGAA GGCAAGAACG TCTACACTGA GATCAAATGC 
AACTCGCTGC TCCCTCTCGA TGATATTGTA CGTGTGGTCA CACATGAGGA CTGCATCCCT 
GAGGTGAAGA TGGCCTACGT GAACTTCCTG AATCACTGTT ATGTTGATAC AGAGGTTGAG 
ATGAAGGAGA TCTACACCAG CAATCACATG TGGAAACTTT TTGATGATTT TCTGGTGGAC 
GTCTGCAGAG TGTGCAATAA CACAAGTGAC CGTAAACACG CTGACACAGT TCTGGAGAGA 
TACGTTACAG AGACCATAAT GAGCATCGTC ACCACCTTTT TCAGCTCACC ATTCTCAGAC 
CAGAGCACAA GTTTGCAGAC CCGGCAGCCG GTGTTTGTGC AGCTGCTGCA GGGAGTGTTT 
CGTGTATATC ACTGCAACTG GCTTCTCCCA GGTCAGAAGG GGAATGTGGA AGCCTGCATC 
AAAGTGCTGA GTGACGTGGC CAAAGGCAGA GCCATAGCCA TTCCTGTGGA TCTGGACTGT 
CAGGTGAATA ATCTGTTCAT GAAGTCCAAC AACATTGTGC AGAAAACAGC CCTCAGCTGG 
AGACTCTCTG TCCGTAACGC GACTCGGAGA GACTCTGTTC TGACCACCTC TCGAGACTAT 
CGCAACATCA TTGAAAGACT ACAGGATATA GTGTCTGCAT TGGAGGAGCG GTTGAGGCCT 
CTGGTTCAGG CTGAGTTGTC AGTGTTGGTG GATGTTCTCC ATCGCCCAGA GCTGCTGTTT 
CCAGAGCACA CTGAAGCCCA CCGCAAGTGT GAGAGTGGAG GCTTCATCTG CAAGTTGATA 
AAACACACTA AGCAGCTTCT AGAAGAAAAC GAAGAGAGGC TTTGCATCAA AGTCCTTCAG 
ACACTCCGAG AGATGATGAC CAAAGACAGA GGATATGGAG AGAAGCTTCT GGGATATGAT 
GATGAGATGG ATGTCACGGA GGTGGTGGAT ATCAGTCTGC CACCCAAACA GCAGGAGGAT 
CGGCAGAGGG GGGAGGCCCT TCGGCAGCTG CTCGTCAATC GTTACTATGG CTTCAGGAGT 
GGTGGGCGGA GAGAAAGTCT GACCTCGTTT GGTAACTCCA CCCTAACTCC TGTCGGGCCA 
ATCAAGAGCC AGCCAGGGGG GTTGAGCAGG GCTGAGATGT CATTAATGGA GGTGCAGTGT 
CATCTAGACC GTGAAGGGGC ATCGGATCTT GTCATTGATC TCATCATGAA CACTACCAGC 
GACCGCGTCT TCCATGAAAG CATTCTGTTG GCCATCGCAC TGTTGGAGGG CGGAAACACC 
ATCATACAGC ATTCCTTCTT CAAACGTTTA ACAGAAGACA AAAATTCAGA GAAGTTTTTC 
AGGGTGTTTT ATGACAGAAT GAAAGTGGCT CAGGTGGAGA TCAAAGCCAC GGTTACTGTC 
AACACTAGTG ACCTCGGCAA CAAGCGGCGT GACGACAACA CTCCAGACAA AGATACTCCA 
CAACGCCGCA GAGGTAAAGA CTCCGGTGTG GTGGTGACAG ATGATGCTCG TGAGCAGCTG 
TTGGAGGCTT CAGCTGCGAC TAAGAAGGCC TTTGGTTCAT ATCGTCGAGA TGCTGATCCC 
GAGGAGACGT TTGGCACGGC TGATGGAGAT AAAGGAGGTG GAGATAAAGG CACAGAGCAG 
GGCGAGATGA GCCCTGTCAT CCTCATAATG CAGCCAATCC TGCGCTTCCT GCAGCTGCTG 
TGCGAAAACC ACAATCGAGA CCTGCAGAAT TTTCTGCGCT GTCAGAACAA TAAAAACAAC 
TATAATCTGG TGTGTGAGAC GCTGCAGTTC CTGGACTGTA TCTGTGGCAG CACAACAGGA 
GGCCTGGGGC TGTTAGGACT CTACATCAAC CAGCATAACG TAGCGCTCAT TAATCAGACT 
GTAGAGAGCC TCACTGAATA CTGTCAAGGT CCCTGCCATG ACAATCAGAA TTGCATTGCC 
ACCCATGAGT CTAATGGCAT CGACATCATT ATTGCACTGA TTCTGAATGA TATTAATCCA 
CTGGGCAGGA AGAGAATGGA TCTGGTTCTA GAACTAAAGA ATAATGCATC TAAGCTGCTG 
CTGGCCATCA TGGAGAGCCG TCATGACAGT GAGAATGCAG AAAGAATTCT GTACAACATG 
AGACCAAAAG AACTAGTGGA GGTGATGAAA ATGGCCTACC AGCAGGGGGA GGCAGAGTTT 
GAGGATGAGG AACAGGAGAA TGGAGAGGAT CATGCAGCAT CGCCTCGCAA TGTTGGACAC 
AACATCTATA TTCTTGCACA TCAGCTGTCG CGTCACAATA AGGAATTGCA AATACTACTG 
AAACCAGGTG GAGAGGACCA GGCCCTGGAG TATTACACAA AACACACCTC ACAGATAGAG 
ATAGTTCGAC AGGATCGCAC TATGGAACAG ATTGTGTTTC CTGTACCAAA TATCTGCTCT 
TTTCTGACAA ATGAGTCCAA GCTGCGTGTG TACTATGGTA CAGAGAGAGA CGAGCAAGGC 
AGTAAAATAA ATGACTTCTT CTTGCATGCA GACGACCTCT TTAATGAAAT GAGGTGGCAG 
AAAAAACTAA GAGCTCAGCC AGTGCTGTAC TGGTGCAGCA GGAACATGTC GTTTTGGAGT 
AACGTTTCCT TTAATCTGGC GGTGCTCATT AATTTGCTGG TGGCATTTTT CTACCCTCTG 
GATGGAGTGA GCGAAAGCCA GCTGGAACCT TCTCTGTCTC TGTTGCTGTG GGTTTGTTTG 
TTGGGCTCAC TGGGCTTCGT GTTGATGTCC CCGAGGCCAA ATGCAGTGCG GGTGCTGGTC 
ATCTCCACTG TCCTGCAGTT GGGCTTCTCT GTAGGGCTGC AGCACATGCT CACACTCCTG 
GGAGCTTTCA ATGTTTGTAA TAAGATCGTG TTCATGCTGA GCTTTGTGGG TAACCGTGGG 
TCATTCACCC GGGGTTATCG GGTGATGGTC ATGGATAGAG AGTTCCTGTT TCATCTGCTG 
TACCTTCTCA TCTGCATACT GGGCCTTTTT GGACACGTCT TCTTTTACAG TCTTCTGTTG 
TTTGACCTGG TGAACCGGGA AGAGACTCTT CTGAACGTCA TCAAGAGTGT GACTCGAAAC 
GGCCGCTCCA TCGTCTTGAC CGCAGTGCTG GGCCTCATCC TGGTCTACCT CTTTTCTATT 
GTTGGATATA TGTTTTTCAA AGATGACTTC ATCCTGGAGG TGAACCGCAT CTCCAACGGC 
ACTCTGGAGG AGGGGGTGAC TTTACCTGCT GGTTTTTGTG ATGCGAGAAA CGGGTCGTGT 
CTTTCTGATG ATGTTGACGA GGATGACGTG GAGCGCGCGT GTGATTCTCT ATGGATGTGT 
ATGATCACTG TACTCAGTCA TGGCCTCAGG AGCGGTGGAG GAGTTGGAGA TGTCCTGCGC 
AAACCGTCTA AAGAGGAGCG TCTGTTCGCC GCACGGGTGG TGTACGATCT CCTGTTCTTC 
TTCCTGGTGA TCATCATTGT GTTGAATCTG ATCTTCGGTG TGATCATTGA CACATTTGCA 
GATCTGAGAA GCGAGAAGCA GAGAAAAGAG GAGATCTTGA AGACCACATG CTTCATCTGT 
GGTCTGGAGA GAGATAAGTT TGATAATAAA ACGGTGACGT TTGAAGAGCA CATCAAGGAG 
GAGCACAACC TGTGGCATTA CCTCTTCTTC ATAGTGCTGG TGCGGGTCAA AGACTCCACC 
GAATACACGG GACCCGAGAG CTACGTCGCT CAGATGATCA AGGAGCACAA TCTGGACTGG 
TTTCCTCGTA TGAGGGCGAT GTCTCTGGTC AGCAGTGATG GAGAAGGTGA ACAGAATGAA 
CTGCGCAGCT TACAGGAGAA ACTCGAGTCC ACAATGAGGC TCGTCTCTAA TCTTACCAAT 
CAGCTCACAG AGCTCAAAGA GCAGATGACT GAGCAAAGGA AACACAAGCA GAGGCTCGGT 
CTGCTGGGAA ACCCCGCCCA CCTCAACATC AACCCACAGC AGCCAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300862.1
CDS	148..8238
Translation	MVDKLSSFLHIGDVCSLYAEGSTSGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGTSTTDTVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDAAGNEGSWFYIQPFYKLRSIGDNVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCTTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDEHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEMDSEHEVIRARARNPQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSPLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAAKLEKGTITQNERRSVTKLLEDLVYFVVDIPSSAQDVLEITVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRYSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDPANADILIETKLVLSRFEVAGTVLGEGAEEEEEDEEEVWLFWKDSGGEVKSKSIRELAQDAKDGHTEDQEVINYYRYQLNLFARMCLDRQYLAINKISAQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSQISIDDYDNDGTSSDEVKERFSQTMEFVENYLRDVVCQSFPFSDKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHISTPFHIKLDREPGKGSNVMRSIHGVGELMTQVVLRGGGFLPASSHNPPDRDEVKAQSEPQKQDILVMDTKLKIIEILQFILNVRLDYRISCLLCIFKTEFDESNSQSEPSLSPESPASVQGALDFEHIEEQAEGIFGGSSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALHLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSDSGLDAGEVTPEAHHKGVINSSRSNKPKVESTSSSNYRVVKEILLRLSKLCVLEGISGKKNKKQQQRLLRNMGAHSVVLELLQIPYEKGEDVQMQEIMTLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAVTMQHIFMNNFQLCSEINERVVQHFVHCIETHGRSVHYLKFLQTIVKAENKFIKKCQDIVMAELVTAGEDVLVFYNDRASFQSLVQMMRLERERLDESSALRYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKMAYVNFLNHCYVDTEVEMKEIYTSNHMWKLFDDFLVDVCRVCNNTSDRKHADTVLERYVTETIMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQGVFRVYHCNWLLPGQKGNVEACIKVLSDVAKGRAIAIPVDLDCQVNNLFMKSNNIVQKTALSWRLSVRNATRRDSVLTTSRDYRNIIERLQDIVSALEERLRPLVQAELSVLVDVLHRPELLFPEHTEAHRKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKLLGYDDEMDVTEVVDISLPPKQQEDRQRGEALRQLLVNRYYGFRSGGRRESLTSFGNSTLTPVGPIKSQPGGLSRAEMSLMEVQCHLDREGASDLVIDLIMNTTSDRVFHESILLAIALLEGGNTIIQHSFFKRLTEDKNSEKFFRVFYDRMKVAQVEIKATVTVNTSDLGNKRRDDNTPDKDTPQRRRGKDSGVVVTDDAREQLLEASAATKKAFGSYRRDADPEETFGTADGDKGGGDKGTEQGEMSPVILIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINQHNVALINQTVESLTEYCQGPCHDNQNCIATHESNGIDIIIALILNDINPLGRKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVMKMAYQQGEAEFEDEEQENGEDHAASPRNVGHNIYILAHQLSRHNKELQILLKPGGEDQALEYYTKHTSQIEIVRQDRTMEQIVFPVPNICSFLTNESKLRVYYGTERDEQGSKINDFFLHADDLFNEMRWQKKLRAQPVLYWCSRNMSFWSNVSFNLAVLINLLVAFFYPLDGVSESQLEPSLSLLLWVCLLGSLGFVLMSPRPNAVRVLVISTVLQLGFSVGLQHMLTLLGAFNVCNKIVFMLSFVGNRGSFTRGYRVMVMDREFLFHLLYLLICILGLFGHVFFYSLLLFDLVNREETLLNVIKSVTRNGRSIVLTAVLGLILVYLFSIVGYMFFKDDFILEVNRISNGTLEEGVTLPAGFCDARNGSCLSDDVDEDDVERACDSLWMCMITVLSHGLRSGGGVGDVLRKPSKEERLFAARVVYDLLFFFLVIIIVLNLIFGVIIDTFADLRSEKQRKEEILKTTCFICGLERDKFDNKTVTFEEHIKEEHNLWHYLFFIVLVRVKDSTEYTGPESYVAQMIKEHNLDWFPRMRAMSLVSSDGEGEQNELRSLQEKLESTMRLVSNLTNQLTELKEQMTEQRKHKQRLGLLGNPAHLNINPQQPA

Target ORF information:

RefSeq Version	XM_009302587.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302587.2

ORF Insert Sequence:

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GATGGATTCG 
GAGCATGAAG TGATTCGGGC TCGTGCACGT AACCCTCAGG ATAAGGTGAT GTACACACTG 
GTGTCTGTTC CTGATGGAAA TGACATATCC TCCATCTTTG AGCTTGACCC TACAACTTTG 
AGGGGCGGAG ACTCACTTGT GCCAAGAAAC TCGTATGTGA GGCTCAGGCA TCTCTGCACC 
AACACGTGGG TCCACAGCAC CAACCAGCCA ATAGATAAAG AGGAAGAGAA ACCGGTCATG 
CTCAGAATAG GAACGTCTCC TCTGAAGGAA GATAAAGAGG CGTTTGCTAT AGTACCAGTC 
TCTCCAGCTG AGGTTCGAGA TCTGGATTTT GCTAATGATG CTAGTAAAGT GTTGGCTTCC 
ATTGCTGCCA AACTGGAGAA GGGCACCATT ACCCAGAATG AGAGGAGGTC AGTGACGAAG 
CTGTTGGAGG ATCTGGTGTA TTTTGTGGTG GACATACCCA GCAGTGCTCA AGATGTGCTG 
GAGATCACAG TCAACAAACC CAACAGAGAG CGACAGAAAC TCATGAGAGA GCAGAACATT 
CTTAAACAGA TCTTCAAGCT GCTGCAGGCG CCATTTACAG ACAGCGGGGA CGGCCCTATG 
TTGCGATTAG AAGAGCTCGC AGACCAGCGT CATGCACCCT TTCGACACAT TTGCAGACTC 
TGTTACAGAG TCCTACGTTA CTCACAACAG GACTATCGCA AAAATCAGGA GTACATAGCA 
AAGCAGTTCC GCTTCATGCA GAAGCAGATA GGATATGATG TGCTGGCTGA AGACACAATT 
ACAGCCCTTC TGCACAACAA CAGGAAGCTC CTAGAGAAAC ACATCACAGC AGCTGAAATA 
GATACCTTTG TCAGTCTGGT CCGCAAGAAC AGAGAGCCCA GATTTCTGGA TTACTTGTCA 
GATCTGTGTG TGTCGATGAA TAAATCCATC CCGGTCACTC AAGAGCTCAT CTGTAATGCT 
GTGCTGGACC CAGCCAATGC AGACATTCTC ATCGAAACCA AGCTTGTGTT GTCACGGTTT 
GAGGTGGCAG GAACGGTGTT GGGTGAAGGT GCGGAGGAGG AAGAGGAGGA TGAAGAGGAG 
GTCTGGCTGT TCTGGAAGGA CAGTGGAGGA GAGGTGAAGA GTAAAAGCAT ACGAGAGCTT 
GCACAGGACG CAAAAGATGG ACATACAGAG GACCAGGAAG TCATCAATTA TTACAGATAT 
CAGCTGAATT TGTTTGCTCG GATGTGTCTG GATCGACAGT ATCTTGCGAT TAACAAGATA 
TCAGCACAGC TGGATGTGGA TCTGATTCTG CGCTGCATGT CAGATGAAGA TTTGCCCTTT 
GACCTCAGAG CATCGTTCTG CCGCATGATG CTGCACATGC ATGTAGACCG TGACCCTCAG 
GAGCAGGTCA CCCCGGTCAA ATATGCTCGA CTGTGGTCTG AAATCCCATC ACAAATTTCC 
ATTGATGATT ATGATAATGA TGGGACGTCA AGTGATGAAG TGAAAGAGCG CTTCTCTCAG 
ACGATGGAGT TTGTGGAGAA TTACCTGAGG GATGTTGTCT GCCAGAGTTT CCCGTTCTCT 
GATAAGGAGA AAAACAAACT CACTTTTGAG GTGGTGAACC TGGCAAGAAA CCTGATCTAT 
TTTGGTTTCT ATAACTTCAG TGATCTGCTA AGGTTAACAA AAATCCTATT GGCAATCCTG 
GACTGTGTCC ACATAAGCAC TCCCTTCCAC ATCAAGCTGG ATAGAGAACC CGGTAAAGGA 
AGTAACGTGA TGCGCTCCAT CCATGGGGTG GGAGAGCTGA TGACACAGGT GGTGTTGAGG 
GGTGGGGGAT TTCTGCCAGC CTCCAGCCAC AATCCTCCTG ACAGAGACGA AGTGAAAGCA 
CAAAGTGAAC CTCAGAAACA GGACATACTG GTGATGGACA CTAAACTCAA GATCATCGAG 
ATCCTGCAGT TTATTCTGAA TGTGCGGTTG GACTACAGGA TCTCCTGCCT CCTCTGTATC 
TTTAAGACAG AGTTTGATGA AAGTAACTCT CAAAGTGAAC CATCTTTAAG CCCAGAATCT 
CCTGCCAGCG TTCAAGGTGC GCTAGACTTT GAGCACATTG AAGAGCAGGC GGAGGGTATT 
TTTGGAGGAA GTAGTGAAGA GAACACCCCG CTGGATCTTG ATGATCATGG TGGACGGACA 
TTTCTGAGGG TCCTGCTTCA CCTGACCATG CATGACTATC CTCCGCTGGT ATCTCGAGCT 
CTTCACCTGC TCTTCAGACA CTTTAGCCAA CGCCAGGAGG TCCTACAGGC CTTCAAACAG 
GTTCAGCTCC TGGTGACCAG TCAGGATGTG GAAAACTATA AACAGATCAA GTCAGATTTG 
GACCAGCTGA GGTCAATAGT AGAGAAATCA GAGTTGTGGG TGTATAAGAG ACAAGGCTCA 
GACTCAGGAC TGGATGCTGG AGAAGTCACC CCTGAAGCCC ACCACAAGGG TGTCATCAAC 
TCGAGTAGAT CAAACAAACC CAAAGTGGAA AGTACCAGCA GCTCGAACTA CAGAGTCGTG 
AAAGAGATCC TGCTGAGGCT GAGCAAACTG TGTGTTTTGG AGGGCATCTC TGGCAAGAAG 
AATAAGAAAC AGCAGCAGCG GCTCCTCAGG AACATGGGAG CTCACAGTGT GGTCCTCGAA 
CTGTTGCAAA TCCCTTATGA GAAGGGGGAG GATGTCCAGA TGCAGGAGAT CATGACCTTA 
GCACATCAGT TCCTTCAAAA CTTCTGTGCT GGAAATCAGC AGAACCAGGC ACTTCTACAC 
AAACACATCA ACCTGTTCCT CAACCCTGGG ATCCTGGAGG CGGTGACCAT GCAGCATATC 
TTCATGAATA ATTTCCAGTT GTGTAGTGAG ATTAATGAGC GGGTCGTGCA GCATTTTGTG 
CACTGCATCG AGACACACGG CCGCAGCGTG CACTACCTCA AGTTCCTGCA GACCATTGTC 
AAGGCAGAGA ACAAGTTCAT CAAGAAATGC CAAGACATCG TCATGGCTGA GCTGGTGACT 
GCAGGTGAGG ATGTGCTGGT GTTCTATAAT GACCGGGCGT CTTTTCAGTC ATTGGTTCAG 
ATGATGCGTC TTGAGCGGGA ACGACTTGAT GAGAGCAGTG CACTCAGGTA TCACATTCAC 
CTGGTGGAGT TGCTGGCTGT GTGCACTGAA GGCAAGAACG TCTACACTGA GATCAAATGC 
AACTCGCTGC TCCCTCTCGA TGATATTGTA CGTGTGGTCA CACATGAGGA CTGCATCCCT 
GAGGTGAAGA TGGCCTACGT GAACTTCCTG AATCACTGTT ATGTTGATAC AGAGGTTGAG 
ATGAAGGAGA TCTACACCAG CAATCACATG TGGAAACTTT TTGATGATTT TCTGGTGGAC 
GTCTGCAGAG TGTGCAATAA CACAAGTGAC CGTAAACACG CTGACACAGT TCTGGAGAGA 
TACGTTACAG AGACCATAAT GAGCATCGTC ACCACCTTTT TCAGCTCACC ATTCTCAGAC 
CAGAGCACAA GTTTGCAGAC CCGGCAGCCG GTGTTTGTGC AGCTGCTGCA GGGAGTGTTT 
CGTGTATATC ACTGCAACTG GCTTCTCCCA GGTCAGAAGG GGAATGTGGA AGCCTGCATC 
AAAGTGCTGA GTGACGTGGC CAAAGGCAGA GCCATAGCCA TTCCTGTGGA TCTGGACTGT 
CAGGTGAATA ATCTGTTCAT GAAGTCCAAC AACATTGTGC AGAAAACAGC CCTCAGCTGG 
AGACTCTCTG TCCGTAACGC GACTCGGAGA GACTCTGTTC TGACCACCTC TCGAGACTAT 
CGCAACATCA TTGAAAGACT ACAGGATATA GTGTCTGCAT TGGAGGAGCG GTTGAGGCCT 
CTGGTTCAGG CTGAGTTGTC AGTGTTGGTG GATGTTCTCC ATCGCCCAGA GCTGCTGTTT 
CCAGAGCACA CTGAAGCCCA CCGCAAGTGT GAGAGTGGAG GCTTCATCTG CAAGTTGATA 
AAACACACTA AGCAGCTTCT AGAAGAAAAC GAAGAGAGGC TTTGCATCAA AGTCCTTCAG 
ACACTCCGAG AGATGATGAC CAAAGACAGA GGATATGGAG AGAAGCTTCT GGGATATGAT 
GATGAGATGG ATGTCACGGA GGTGGTGGAT ATCAGTCTGC CACCCAAACA GCAGGAGGAT 
CGGCAGAGGG GGGAGGCCCT TCGGCAGCTG CTCGTCAATC GTTACTATGG CTTCAGGAGT 
GGTGGGCGGA GAGAAAGTCT GACCTCGTTT GGTAACTCCA CCCTAACTCC TGTCGGGCCA 
ATCAAGAGCC AGCCAGGGGG GTTGAGCAGG GCTGAGATGT CATTAATGGA GGTGCAGTGT 
CATCTAGACC GTGAAGGGGC ATCGGATCTT GTCATTGATC TCATCATGAA CACTACCAGC 
GACCGCGTCT TCCATGAAAG CATTCTGTTG GCCATCGCAC TGTTGGAGGG CGGAAACACC 
ATCATACAGC ATTCCTTCTT CAAACGTTTA ACAGAAGACA AAAATTCAGA GAAGTTTTTC 
AGGGTGTTTT ATGACAGAAT GAAAGTGGCT CAGGTGGAGA TCAAAGCCAC GGTTACTGTC 
AACACTAGTG ACCTCGGCAA CAAGCGGCGT GACGACAACA CTCCAGACAA AGATACTCCA 
CAACGCCGCA GAGGTAAAGA CTCCGGTGTG GTGGTGACAG ATGATGCTCG TGAGCAGCTG 
TTGGAGGCTT CAGCTGCGAC TAAGAAGGCC TTTGGTTCAT ATCGTCGAGA TGCTGATCCC 
GAGGAGACGT TTGGCACGGC TGATGGAGAT AAAGGAGGTG GAGATAAAGG CACAGAGCAG 
GGCGAGATGA GCCCTGTCAT CCTCATAATG CAGCCAATCC TGCGCTTCCT GCAGCTGCTG 
TGCGAAAACC ACAATCGAGA CCTGCAGAAT TTTCTGCGCT GTCAGAACAA TAAAAACAAC 
TATAATCTGG TGTGTGAGAC GCTGCAGTTC CTGGACTGTA TCTGTGGCAG CACAACAGGA 
GGCCTGGGGC TGTTAGGACT CTACATCAAC CAGCATAACG TAGCGCTCAT TAATCAGACT 
GTAGAGAGCC TCACTGAATA CTGTCAAGGT CCCTGCCATG ACAATCAGAA TTGCATTGCC 
ACCCATGAGT CTAATGGCAT CGACATCATT ATTGCACTGA TTCTGAATGA TATTAATCCA 
CTGGGCAGGA AGAGAATGGA TCTGGTTCTA GAACTAAAGA ATAATGCATC TAAGCTGCTG 
CTGGCCATCA TGGAGAGCCG TCATGACAGT GAGAATGCAG AAAGAATTCT GTACAACATG 
AGACCAAAAG AACTAGTGGA GGTGATGAAA ATGGCCTACC AGCAGGGGGA GGCAGAGTTT 
GAGGATGAGG AACAGGAGAA TGGAGAGGAT CATGCAGCAT CGCCTCGCAA TGTTGGACAC 
AACATCTATA TTCTTGCACA TCAGCTGTCG CGTCACAATA AGGAATTGCA AATACTACTG 
AAACCAGGTG GAGAGGACCA GGCCCTGGAG TATTACACAA AACACACCTC ACAGATAGAG 
ATAGTTCGAC AGGATCGCAC TATGGAACAG ATTGTGTTTC CTGTACCAAA TATCTGCTCT 
TTTCTGACAA ATGAGTCCAA GCTGCGTGTG TACTATGGTA CAGAGAGAGA CGAGCAAGGC 
AGTAAAATAA ATGACTTCTT CTTGCATGCA GACGACCTCT TTAATGAAAT GAGGTGGCAG 
AAAAAACTAA GAGCTCAGCC AGTGCTGTAC TGGTGCAGCA GGAACATGTC GTTTTGGAGT 
AACGTTTCCT TTAATCTGGC GGTGCTCATT AATTTGCTGG TGGCATTTTT CTACCCTCTG 
GATGGAGTGA GCGAAAGCCA GCTGGAACCT TCTCTGTCTC TGTTGCTGTG GGTTTGTTTG 
TTGGGCTCAC TGGGCTTCGT GTTGATGTCC CCGAGGCCAA ATGCAGTGCG GGTGCTGGTC 
ATCTCCACTG TCCTGCAGTT GGGCTTCTCT GTAGGGCTGC AGCACATGCT CACACTCCTG 
GGAGCTTTCA ATGTTTGTAA TAAGATCGTG TTCATGCTGA GCTTTGTGGG TAACCGTGGG 
TCATTCACCC GGGGTTATCG GGTGATGGTC ATGGATAGAG AGTTCCTGTT TCATCTGCTG 
TACCTTCTCA TCTGCATACT GGGCCTTTTT GGACACGTCT TCTTTTACAG TCTTCTGTTG 
TTTGACCTGG TGAACCGGGA AGAGACTCTT CTGAACGTCA TCAAGAGTGT GACTCGAAAC 
GGCCGCTCCA TCGTCTTGAC CGCAGTGCTG GGCCTCATCC TGGTCTACCT CTTTTCTATT 
GTTGGATATA TGTTTTTCAA AGATGACTTC ATCCTGGAGG TGAACCGCAT CTCCAACGGC 
ACTCTGGAGG AGGGGGTGAC TTTACCTGCT GGTTTTTGTG ATGCGAGAAA CGGGTCGTGT 
CTTTCTGATG ATGTTGACGA GGATGACGTG GAGCGCGCGT GTGATTCTCT ATGGATGTGT 
ATGATCACTG TACTCAGTCA TGGCCTCAGG AGCGGTGGAG GAGTTGGAGA TGTCCTGCGC 
AAACCGTCTA AAGAGGAGCG TCTGTTCGCC GCACGGGTGG TGTACGATCT CCTGTTCTTC 
TTCCTGGTGA TCATCATTGT GTTGAATCTG ATCTTCGGTG TGATCATTGA CACATTTGCA 
GATCTGAGAA GCGAGAAGCA GAGAAAAGAG GAGATCTTGA AGACCACATG CTTCATCTGT 
GGTCTGGAGA GAGATAAGTT TGATAATAAA ACGGTGACGT TTGAAGAGCA CATCAAGGAG 
GAGCACAACC TGTGGCATTA CCTCTTCTTC ATAGTGCTGG TGCGGGTCAA AGACTCCACC 
GAATACACGG GACCCGAGAG CTACGTCGCT CAGATGATCA AGGAGCACAA TCTGGACTGG 
TTTCCTCGTA TGAGGGCGAT GTCTCTGGTC AGCAGTGATG GAGAAGGTGA ACAGAATGAA 
CTGCGCAGCT TACAGGAGAA ACTCGAGTCC ACAATGAGGC TCGTCTCTAA TCTTACCAAT 
CAGCTCACAG AGCTCAAAGA GCAGATGACT GAGCAAAGGA AACACAAGCA GAGGCTCGGT 
CTGCTGGGAA ACCCCGCCCA CCTCAACATC AACCCACAGC AGCCAGCATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itpr1a ( XM_009302588.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300863.1 Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X5, mRNA.

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CloneID	ODa45195
Clone ID Related Accession (Same CDS sequence)	XM_009302588.3 , XM_009302588.2
Accession Version	XM_009302588.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009302588.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GGTAAATCCA 
GACTATGAAG AAGAATGCCT GGAGTCCCGC TCCTCTATGG ATTCGGAGCA TGAAGTGATT 
CGGGCTCGTG CACGTAACCC TCAGGATAAG GTGATGTACA CACTGGTGTC TGTTCCTGAT 
GGAAATGACA TATCCTCCAT CTTTGAGCTT GACCCTACAA CTTTGAGGGG CGGAGACTCA 
CTTGTGCCAA GAAACTCGTA TGTGAGGCTC AGGCATCTCT GCACCAACAC GTGGGTCCAC 
AGCACCAACC AGCCAATAGA TAAAGAGGAA GAGAAACCGG TCATGCTCAG AATAGGAACG 
TCTCCTCTGA AGGAAGATAA AGAGGCGTTT GCTATAGTAC CAGTCTCTCC AGCTGAGGTT 
CGAGATCTGG ATTTTGCTAA TGATGCTAGT AAAGTGTTGG CTTCCATTGC TGCCAAACTG 
GAGAAGGGCA CCATTACCCA GAATGAGAGG AGGTCAGTGA CGAAGCTGTT GGAGGATCTG 
GTGTATTTTG TGGTGGACAT ACCCAGCAGT GCTCAAGATG TGCTGGAGAT CACAGTCAAC 
AAACCCAACA GAGAGCGACA GAAACTCATG AGAGAGCAGA ACATTCTTAA ACAGATCTTC 
AAGCTGCTGC AGGCGCCATT TACAGACAGC GGGGACGGCC CTATGTTGCG ATTAGAAGAG 
CTCGCAGACC AGCGTCATGC ACCCTTTCGA CACATTTGCA GACTCTGTTA CAGAGTCCTA 
CGTTACTCAC AACAGGACTA TCGCAAAAAT CAGGAGTACA TAGCAAAGCA GTTCCGCTTC 
ATGCAGAAGC AGATAGGATA TGATGTGCTG GCTGAAGACA CAATTACAGC CCTTCTGCAC 
AACAACAGGA AGCTCCTAGA GAAACACATC ACAGCAGCTG AAATAGATAC CTTTGTCAGT 
CTGGTCCGCA AGAACAGAGA GCCCAGATTT CTGGATTACT TGTCAGATCT GTGTGTGTCG 
ATGAATAAAT CCATCCCGGT CACTCAAGAG CTCATCTGTA ATGCTGTGCT GGACCCAGCC 
AATGCAGACA TTCTCATCGA AACCAAGCTT GTGTTGTCAC GGTTTGAGGT GGCAGGAACG 
GTGTTGGGTG AAGGTGCGGA GGAGGAAGAG GAGGATGAAG AGGAGGTCTG GCTGTTCTGG 
AAGGACAGTG GAGGAGAGGT GAAGAGTAAA AGCATACGAG AGCTTGCACA GGACGCAAAA 
GATGGACATA CAGAGGACCA GGAAGTCATC AATTATTACA GATATCAGCT GAATTTGTTT 
GCTCGGATGT GTCTGGATCG ACAGTATCTT GCGATTAACA AGATATCAGC ACAGCTGGAT 
GTGGATCTGA TTCTGCGCTG CATGTCAGAT GAAGATTTGC CCTTTGACCT CAGAGCATCG 
TTCTGCCGCA TGATGCTGCA CATGCATGTA GACCGTGACC CTCAGGAGCA GGTCACCCCG 
GTCAAATATG CTCGACTGTG GTCTGAAATC CCATCACAAA TTTCCATTGA TGATTATGAT 
AATGATGGGA CGTCAAGTGA TGAAGTGAAA GAGCGCTTCT CTCAGACGAT GGAGTTTGTG 
GAGAATTACC TGAGGGATGT TGTCTGCCAG AGTTTCCCGT TCTCTGATAA GGAGAAAAAC 
AAACTCACTT TTGAGGTGGT GAACCTGGCA AGAAACCTGA TCTATTTTGG TTTCTATAAC 
TTCAGTGATC TGCTAAGGTT AACAAAAATC CTATTGGCAA TCCTGGACTG TGTCCACATA 
AGCACTCCCT TCCACATCAA GCTGGATAGA GAACCCGGTA AAGGAAGTAA CGTGATGCGC 
TCCATCCATG GGGTGGGAGA GCTGATGACA CAGGTGGTGT TGAGGGGTGG GGGATTTCTG 
CCAGCCTCCA GCCACAATCC TCCTGACAGA GACGAAGTGA AAGCACAAAG TGAACCTCAG 
AAACAGGACA TACTGGTGAT GGACACTAAA CTCAAGATCA TCGAGATCCT GCAGTTTATT 
CTGAATGTGC GGTTGGACTA CAGGATCTCC TGCCTCCTCT GTATCTTTAA GACAGAGTTT 
GATGAAAGTA ACTCTCAAAG TGAACCATCT TTAAGCCCAG AATCTCCTGC CAGCGTTCAA 
GGTGCGCTAG ACTTTGAGCA CATTGAAGAG CAGGCGGAGG GTATTTTTGG AGGAAGTAGT 
GAAGAGAACA CCCCGCTGGA TCTTGATGAT CATGGTGGAC GGACATTTCT GAGGGTCCTG 
CTTCACCTGA CCATGCATGA CTATCCTCCG CTGGTATCTC GAGCTCTTCA CCTGCTCTTC 
AGACACTTTA GCCAACGCCA GGAGGTCCTA CAGGCCTTCA AACAGGTTCA GCTCCTGGTG 
ACCAGTCAGG ATGTGGAAAA CTATAAACAG ATCAAGTCAG ATTTGGACCA GCTGAGGTCA 
ATAGTAGAGA AATCAGAGTT GTGGGTGTAT AAGAGACAAG GCTCAGACTC AGGACTGGAT 
GCTGGAGAAG TCACCCCTGA AGCCCACCAC AAGGGTGTCA TCAACTCGAG TAGATCAAAC 
AAACCCAAAG TGGAAAGTAC CAGCAGCTCG AACTACAGAG TCGTGAAAGA GATCCTGCTG 
AGGCTGAGCA AACTGTGTGT TTTGGAGGGC ATCTCTGGCA AGAAGAATAA GAAACAGCAG 
CAGCGGCTCC TCAGGAACAT GGGAGCTCAC AGTGTGGTCC TCGAACTGTT GCAAATCCCT 
TATGAGAAGG GGGAGGATGT CCAGATGCAG GAGATCATGA CCTTAGCACA TCAGTTCCTT 
CAAAACTTCT GTGCTGGAAA TCAGCAGAAC CAGGCACTTC TACACAAACA CATCAACCTG 
TTCCTCAACC CTGGGATCCT GGAGGCGGTG ACCATGCAGC ATATCTTCAT GAATAATTTC 
CAGTTGTGTA GTGAGATTAA TGAGCGGGTC GTGCAGCATT TTGTGCACTG CATCGAGACA 
CACGGCCGCA GCGTGCACTA CCTCAAGTTC CTGCAGACCA TTGTCAAGGC AGAGAACAAG 
TTCATCAAGA AATGCCAAGA CATCGTCATG GCTGAGCTGG TGACTGCAGG TGAGGATGTG 
CTGGTGTTCT ATAATGACCG GGCGTCTTTT CAGTCATTGG TTCAGATGAT GCGTCTTGAG 
CGGGAACGAC TTGATGAGAG CAGTGCACTC AGGTATCACA TTCACCTGGT GGAGTTGCTG 
GCTGTGTGCA CTGAAGGCAA GAACGTCTAC ACTGAGATCA AATGCAACTC GCTGCTCCCT 
CTCGATGATA TTGTACGTGT GGTCACACAT GAGGACTGCA TCCCTGAGGT GAAGATGGCC 
TACGTGAACT TCCTGAATCA CTGTTATGTT GATACAGAGG TTGAGATGAA GGAGATCTAC 
ACCAGCAATC ACATGTGGAA ACTTTTTGAT GATTTTCTGG TGGACGTCTG CAGAGTGTGC 
AATAACACAA GTGACCGTAA ACACGCTGAC ACAGTTCTGG AGAGATACGT TACAGAGACC 
ATAATGAGCA TCGTCACCAC CTTTTTCAGC TCACCATTCT CAGACCAGAG CACAAGTTTG 
CAGACCCGGC AGCCGGTGTT TGTGCAGCTG CTGCAGGGAG TGTTTCGTGT ATATCACTGC 
AACTGGCTTC TCCCAGGTCA GAAGGGGAAT GTGGAAGCCT GCATCAAAGT GCTGAGTGAC 
GTGGCCAAAG GCAGAGCCAT AGCCATTCCT GTGGATCTGG ACTGTCAGGT GAATAATCTG 
TTCATGAAGT CCAACAACAT TGTGCAGAAA ACAGCCCTCA GCTGGAGACT CTCTGTCCGT 
AACGCGACTC GGAGAGACTC TGTTCTGACC ACCTCTCGAG ACTATCGCAA CATCATTGAA 
AGACTACAGG ATATAGTGTC TGCATTGGAG GAGCGGTTGA GGCCTCTGGT TCAGGCTGAG 
TTGTCAGTGT TGGTGGATGT TCTCCATCGC CCAGAGCTGC TGTTTCCAGA GCACACTGAA 
GCCCACCGCA AGTGTGAGAG TGGAGGCTTC ATCTGCAAGT TGATAAAACA CACTAAGCAG 
CTTCTAGAAG AAAACGAAGA GAGGCTTTGC ATCAAAGTCC TTCAGACACT CCGAGAGATG 
ATGACCAAAG ACAGAGGATA TGGAGAGAAG GGGGAGGCCC TTCGGCAGCT GCTCGTCAAT 
CGTTACTATG GCTTCAGGAG TGGTGGGCGG AGAGAAAGTC TGACCTCGTT TGGTAACTCC 
ACCCTAACTC CTGTCGGGCC AATCAAGAGC CAGCCAGGGG GGTTGAGCAG GGCTGAGATG 
TCATTAATGG AGGTGCAGTG TCATCTAGAC CGTGAAGGGG CATCGGATCT TGTCATTGAT 
CTCATCATGA ACACTACCAG CGACCGCGTC TTCCATGAAA GCATTCTGTT GGCCATCGCA 
CTGTTGGAGG GCGGAAACAC CATCATACAG CATTCCTTCT TCAAACGTTT AACAGAAGAC 
AAAAATTCAG AGAAGTTTTT CAGGGTGTTT TATGACAGAA TGAAAGTGGC TCAGGTGGAG 
ATCAAAGCCA CGGTTACTGT CAACACTAGT GACCTCGGCA ACAAGCGGCG TGACGACAAC 
ACTCCAGACA AAGATACTCC ACAACGCCGC AGAGGTAAAG ACTCCGGTGT GGTGGTGACA 
GATGATGCTC GTGAGCAGCT GTTGGAGGCT TCAGCTGCGA CTAAGAAGGC CTTTGGTTCA 
TATCGTCGAG ATGCTGATCC CGAGGAGACG TTTGGCACGG CTGATGGAGA TAAAGGAGGT 
GGAGATAAAG GCACAGAGCA GGGCGAGATG AGCCCTGTCA TCCTCATAAT GCAGCCAATC 
CTGCGCTTCC TGCAGCTGCT GTGCGAAAAC CACAATCGAG ACCTGCAGAA TTTTCTGCGC 
TGTCAGAACA ATAAAAACAA CTATAATCTG GTGTGTGAGA CGCTGCAGTT CCTGGACTGT 
ATCTGTGGCA GCACAACAGG AGGCCTGGGG CTGTTAGGAC TCTACATCAA CCAGCATAAC 
GTAGCGCTCA TTAATCAGAC TGTAGAGAGC CTCACTGAAT ACTGTCAAGG TCCCTGCCAT 
GACAATCAGA ATTGCATTGC CACCCATGAG TCTAATGGCA TCGACATCAT TATTGCACTG 
ATTCTGAATG ATATTAATCC ACTGGGCAGG AAGAGAATGG ATCTGGTTCT AGAACTAAAG 
AATAATGCAT CTAAGCTGCT GCTGGCCATC ATGGAGAGCC GTCATGACAG TGAGAATGCA 
GAAAGAATTC TGTACAACAT GAGACCAAAA GAACTAGTGG AGGTGATGAA AATGGCCTAC 
CAGCAGGGGG AGGCAGAGTT TGAGGATGAG GAACAGGAGA ATGGAGAGGA TCATGCAGCA 
TCGCCTCGCA ATGTTGGACA CAACATCTAT ATTCTTGCAC ATCAGCTGTC GCGTCACAAT 
AAGGAATTGC AAATACTACT GAAACCAGGT GGAGAGGACC AGGCCCTGGA GTATTACACA 
AAACACACCT CACAGATAGA GATAGTTCGA CAGGATCGCA CTATGGAACA GATTGTGTTT 
CCTGTACCAA ATATCTGCTC TTTTCTGACA AATGAGTCCA AGCTGCGTGT GTACTATGGT 
ACAGAGAGAG ACGAGCAAGG CAGTAAAATA AATGACTTCT TCTTGCATGC AGACGACCTC 
TTTAATGAAA TGAGGTGGCA GAAAAAACTA AGAGCTCAGC CAGTGCTGTA CTGGTGCAGC 
AGGAACATGT CGTTTTGGAG TAACGTTTCC TTTAATCTGG CGGTGCTCAT TAATTTGCTG 
GTGGCATTTT TCTACCCTCT GGATGGAGTG AGCGAAAGCC AGCTGGAACC TTCTCTGTCT 
CTGTTGCTGT GGGTTTGTTT GTTGGGCTCA CTGGGCTTCG TGTTGATGTC CCCGAGGCCA 
AATGCAGTGC GGGTGCTGGT CATCTCCACT GTCCTGCAGT TGGGCTTCTC TGTAGGGCTG 
CAGCACATGC TCACACTCCT GGGAGCTTTC AATGTTTGTA ATAAGATCGT GTTCATGCTG 
AGCTTTGTGG GTAACCGTGG GTCATTCACC CGGGGTTATC GGGTGATGGT CATGGATAGA 
GAGTTCCTGT TTCATCTGCT GTACCTTCTC ATCTGCATAC TGGGCCTTTT TGGACACGTC 
TTCTTTTACA GTCTTCTGTT GTTTGACCTG GTGAACCGGG AAGAGACTCT TCTGAACGTC 
ATCAAGAGTG TGACTCGAAA CGGCCGCTCC ATCGTCTTGA CCGCAGTGCT GGGCCTCATC 
CTGGTCTACC TCTTTTCTAT TGTTGGATAT ATGTTTTTCA AAGATGACTT CATCCTGGAG 
GTGAACCGCA TCTCCAACGG CACTCTGGAG GAGGGGGTGA CTTTACCTGC TGGTTTTTGT 
GATGCGAGAA ACGGGTCGTG TCTTTCTGAT GATGTTGACG AGGATGACGT GGAGCGCGCG 
TGTGATTCTC TATGGATGTG TATGATCACT GTACTCAGTC ATGGCCTCAG GAGCGGTGGA 
GGAGTTGGAG ATGTCCTGCG CAAACCGTCT AAAGAGGAGC GTCTGTTCGC CGCACGGGTG 
GTGTACGATC TCCTGTTCTT CTTCCTGGTG ATCATCATTG TGTTGAATCT GATCTTCGGT 
GTGATCATTG ACACATTTGC AGATCTGAGA AGCGAGAAGC AGAGAAAAGA GGAGATCTTG 
AAGACCACAT GCTTCATCTG TGGTCTGGAG AGAGATAAGT TTGATAATAA AACGGTGACG 
TTTGAAGAGC ACATCAAGGA GGAGCACAAC CTGTGGCATT ACCTCTTCTT CATAGTGCTG 
GTGCGGGTCA AAGACTCCAC CGAATACACG GGACCCGAGA GCTACGTCGC TCAGATGATC 
AAGGAGCACA ATCTGGACTG GTTTCCTCGT ATGAGGGCGA TGTCTCTGGT CAGCAGTGAT 
GGAGAAGGTG AACAGAATGA ACTGCGCAGC TTACAGGAGA AACTCGAGTC CACAATGAGG 
CTCGTCTCTA ATCTTACCAA TCAGCTCACA GAGCTCAAAG AGCAGATGAC TGAGCAAAGG 
AAACACAAGC AGAGGCTCGG TCTGCTGGGA AACCCCGCCC ACCTCAACAT CAACCCACAG 
CAGCCAGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300863.1
CDS	148..8199
Translation	MVDKLSSFLHIGDVCSLYAEGSTSGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGTSTTDTVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDAAGNEGSWFYIQPFYKLRSIGDNVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCTTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDEHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEVNPDYEEECLESRSSMDSEHEVIRARARNPQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSPLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAAKLEKGTITQNERRSVTKLLEDLVYFVVDIPSSAQDVLEITVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRYSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDPANADILIETKLVLSRFEVAGTVLGEGAEEEEEDEEEVWLFWKDSGGEVKSKSIRELAQDAKDGHTEDQEVINYYRYQLNLFARMCLDRQYLAINKISAQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSQISIDDYDNDGTSSDEVKERFSQTMEFVENYLRDVVCQSFPFSDKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHISTPFHIKLDREPGKGSNVMRSIHGVGELMTQVVLRGGGFLPASSHNPPDRDEVKAQSEPQKQDILVMDTKLKIIEILQFILNVRLDYRISCLLCIFKTEFDESNSQSEPSLSPESPASVQGALDFEHIEEQAEGIFGGSSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALHLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSDSGLDAGEVTPEAHHKGVINSSRSNKPKVESTSSSNYRVVKEILLRLSKLCVLEGISGKKNKKQQQRLLRNMGAHSVVLELLQIPYEKGEDVQMQEIMTLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAVTMQHIFMNNFQLCSEINERVVQHFVHCIETHGRSVHYLKFLQTIVKAENKFIKKCQDIVMAELVTAGEDVLVFYNDRASFQSLVQMMRLERERLDESSALRYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKMAYVNFLNHCYVDTEVEMKEIYTSNHMWKLFDDFLVDVCRVCNNTSDRKHADTVLERYVTETIMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQGVFRVYHCNWLLPGQKGNVEACIKVLSDVAKGRAIAIPVDLDCQVNNLFMKSNNIVQKTALSWRLSVRNATRRDSVLTTSRDYRNIIERLQDIVSALEERLRPLVQAELSVLVDVLHRPELLFPEHTEAHRKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKGEALRQLLVNRYYGFRSGGRRESLTSFGNSTLTPVGPIKSQPGGLSRAEMSLMEVQCHLDREGASDLVIDLIMNTTSDRVFHESILLAIALLEGGNTIIQHSFFKRLTEDKNSEKFFRVFYDRMKVAQVEIKATVTVNTSDLGNKRRDDNTPDKDTPQRRRGKDSGVVVTDDAREQLLEASAATKKAFGSYRRDADPEETFGTADGDKGGGDKGTEQGEMSPVILIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINQHNVALINQTVESLTEYCQGPCHDNQNCIATHESNGIDIIIALILNDINPLGRKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVMKMAYQQGEAEFEDEEQENGEDHAASPRNVGHNIYILAHQLSRHNKELQILLKPGGEDQALEYYTKHTSQIEIVRQDRTMEQIVFPVPNICSFLTNESKLRVYYGTERDEQGSKINDFFLHADDLFNEMRWQKKLRAQPVLYWCSRNMSFWSNVSFNLAVLINLLVAFFYPLDGVSESQLEPSLSLLLWVCLLGSLGFVLMSPRPNAVRVLVISTVLQLGFSVGLQHMLTLLGAFNVCNKIVFMLSFVGNRGSFTRGYRVMVMDREFLFHLLYLLICILGLFGHVFFYSLLLFDLVNREETLLNVIKSVTRNGRSIVLTAVLGLILVYLFSIVGYMFFKDDFILEVNRISNGTLEEGVTLPAGFCDARNGSCLSDDVDEDDVERACDSLWMCMITVLSHGLRSGGGVGDVLRKPSKEERLFAARVVYDLLFFFLVIIIVLNLIFGVIIDTFADLRSEKQRKEEILKTTCFICGLERDKFDNKTVTFEEHIKEEHNLWHYLFFIVLVRVKDSTEYTGPESYVAQMIKEHNLDWFPRMRAMSLVSSDGEGEQNELRSLQEKLESTMRLVSNLTNQLTELKEQMTEQRKHKQRLGLLGNPAHLNINPQQPA

Target ORF information:

RefSeq Version	XM_009302588.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302588.3

ORF Insert Sequence:

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GGTAAATCCA 
GACTATGAAG AAGAATGCCT GGAGTCCCGC TCCTCTATGG ATTCGGAGCA TGAAGTGATT 
CGGGCTCGTG CACGTAACCC TCAGGATAAG GTGATGTACA CACTGGTGTC TGTTCCTGAT 
GGAAATGACA TATCCTCCAT CTTTGAGCTT GACCCTACAA CTTTGAGGGG CGGAGACTCA 
CTTGTGCCAA GAAACTCGTA TGTGAGGCTC AGGCATCTCT GCACCAACAC GTGGGTCCAC 
AGCACCAACC AGCCAATAGA TAAAGAGGAA GAGAAACCGG TCATGCTCAG AATAGGAACG 
TCTCCTCTGA AGGAAGATAA AGAGGCGTTT GCTATAGTAC CAGTCTCTCC AGCTGAGGTT 
CGAGATCTGG ATTTTGCTAA TGATGCTAGT AAAGTGTTGG CTTCCATTGC TGCCAAACTG 
GAGAAGGGCA CCATTACCCA GAATGAGAGG AGGTCAGTGA CGAAGCTGTT GGAGGATCTG 
GTGTATTTTG TGGTGGACAT ACCCAGCAGT GCTCAAGATG TGCTGGAGAT CACAGTCAAC 
AAACCCAACA GAGAGCGACA GAAACTCATG AGAGAGCAGA ACATTCTTAA ACAGATCTTC 
AAGCTGCTGC AGGCGCCATT TACAGACAGC GGGGACGGCC CTATGTTGCG ATTAGAAGAG 
CTCGCAGACC AGCGTCATGC ACCCTTTCGA CACATTTGCA GACTCTGTTA CAGAGTCCTA 
CGTTACTCAC AACAGGACTA TCGCAAAAAT CAGGAGTACA TAGCAAAGCA GTTCCGCTTC 
ATGCAGAAGC AGATAGGATA TGATGTGCTG GCTGAAGACA CAATTACAGC CCTTCTGCAC 
AACAACAGGA AGCTCCTAGA GAAACACATC ACAGCAGCTG AAATAGATAC CTTTGTCAGT 
CTGGTCCGCA AGAACAGAGA GCCCAGATTT CTGGATTACT TGTCAGATCT GTGTGTGTCG 
ATGAATAAAT CCATCCCGGT CACTCAAGAG CTCATCTGTA ATGCTGTGCT GGACCCAGCC 
AATGCAGACA TTCTCATCGA AACCAAGCTT GTGTTGTCAC GGTTTGAGGT GGCAGGAACG 
GTGTTGGGTG AAGGTGCGGA GGAGGAAGAG GAGGATGAAG AGGAGGTCTG GCTGTTCTGG 
AAGGACAGTG GAGGAGAGGT GAAGAGTAAA AGCATACGAG AGCTTGCACA GGACGCAAAA 
GATGGACATA CAGAGGACCA GGAAGTCATC AATTATTACA GATATCAGCT GAATTTGTTT 
GCTCGGATGT GTCTGGATCG ACAGTATCTT GCGATTAACA AGATATCAGC ACAGCTGGAT 
GTGGATCTGA TTCTGCGCTG CATGTCAGAT GAAGATTTGC CCTTTGACCT CAGAGCATCG 
TTCTGCCGCA TGATGCTGCA CATGCATGTA GACCGTGACC CTCAGGAGCA GGTCACCCCG 
GTCAAATATG CTCGACTGTG GTCTGAAATC CCATCACAAA TTTCCATTGA TGATTATGAT 
AATGATGGGA CGTCAAGTGA TGAAGTGAAA GAGCGCTTCT CTCAGACGAT GGAGTTTGTG 
GAGAATTACC TGAGGGATGT TGTCTGCCAG AGTTTCCCGT TCTCTGATAA GGAGAAAAAC 
AAACTCACTT TTGAGGTGGT GAACCTGGCA AGAAACCTGA TCTATTTTGG TTTCTATAAC 
TTCAGTGATC TGCTAAGGTT AACAAAAATC CTATTGGCAA TCCTGGACTG TGTCCACATA 
AGCACTCCCT TCCACATCAA GCTGGATAGA GAACCCGGTA AAGGAAGTAA CGTGATGCGC 
TCCATCCATG GGGTGGGAGA GCTGATGACA CAGGTGGTGT TGAGGGGTGG GGGATTTCTG 
CCAGCCTCCA GCCACAATCC TCCTGACAGA GACGAAGTGA AAGCACAAAG TGAACCTCAG 
AAACAGGACA TACTGGTGAT GGACACTAAA CTCAAGATCA TCGAGATCCT GCAGTTTATT 
CTGAATGTGC GGTTGGACTA CAGGATCTCC TGCCTCCTCT GTATCTTTAA GACAGAGTTT 
GATGAAAGTA ACTCTCAAAG TGAACCATCT TTAAGCCCAG AATCTCCTGC CAGCGTTCAA 
GGTGCGCTAG ACTTTGAGCA CATTGAAGAG CAGGCGGAGG GTATTTTTGG AGGAAGTAGT 
GAAGAGAACA CCCCGCTGGA TCTTGATGAT CATGGTGGAC GGACATTTCT GAGGGTCCTG 
CTTCACCTGA CCATGCATGA CTATCCTCCG CTGGTATCTC GAGCTCTTCA CCTGCTCTTC 
AGACACTTTA GCCAACGCCA GGAGGTCCTA CAGGCCTTCA AACAGGTTCA GCTCCTGGTG 
ACCAGTCAGG ATGTGGAAAA CTATAAACAG ATCAAGTCAG ATTTGGACCA GCTGAGGTCA 
ATAGTAGAGA AATCAGAGTT GTGGGTGTAT AAGAGACAAG GCTCAGACTC AGGACTGGAT 
GCTGGAGAAG TCACCCCTGA AGCCCACCAC AAGGGTGTCA TCAACTCGAG TAGATCAAAC 
AAACCCAAAG TGGAAAGTAC CAGCAGCTCG AACTACAGAG TCGTGAAAGA GATCCTGCTG 
AGGCTGAGCA AACTGTGTGT TTTGGAGGGC ATCTCTGGCA AGAAGAATAA GAAACAGCAG 
CAGCGGCTCC TCAGGAACAT GGGAGCTCAC AGTGTGGTCC TCGAACTGTT GCAAATCCCT 
TATGAGAAGG GGGAGGATGT CCAGATGCAG GAGATCATGA CCTTAGCACA TCAGTTCCTT 
CAAAACTTCT GTGCTGGAAA TCAGCAGAAC CAGGCACTTC TACACAAACA CATCAACCTG 
TTCCTCAACC CTGGGATCCT GGAGGCGGTG ACCATGCAGC ATATCTTCAT GAATAATTTC 
CAGTTGTGTA GTGAGATTAA TGAGCGGGTC GTGCAGCATT TTGTGCACTG CATCGAGACA 
CACGGCCGCA GCGTGCACTA CCTCAAGTTC CTGCAGACCA TTGTCAAGGC AGAGAACAAG 
TTCATCAAGA AATGCCAAGA CATCGTCATG GCTGAGCTGG TGACTGCAGG TGAGGATGTG 
CTGGTGTTCT ATAATGACCG GGCGTCTTTT CAGTCATTGG TTCAGATGAT GCGTCTTGAG 
CGGGAACGAC TTGATGAGAG CAGTGCACTC AGGTATCACA TTCACCTGGT GGAGTTGCTG 
GCTGTGTGCA CTGAAGGCAA GAACGTCTAC ACTGAGATCA AATGCAACTC GCTGCTCCCT 
CTCGATGATA TTGTACGTGT GGTCACACAT GAGGACTGCA TCCCTGAGGT GAAGATGGCC 
TACGTGAACT TCCTGAATCA CTGTTATGTT GATACAGAGG TTGAGATGAA GGAGATCTAC 
ACCAGCAATC ACATGTGGAA ACTTTTTGAT GATTTTCTGG TGGACGTCTG CAGAGTGTGC 
AATAACACAA GTGACCGTAA ACACGCTGAC ACAGTTCTGG AGAGATACGT TACAGAGACC 
ATAATGAGCA TCGTCACCAC CTTTTTCAGC TCACCATTCT CAGACCAGAG CACAAGTTTG 
CAGACCCGGC AGCCGGTGTT TGTGCAGCTG CTGCAGGGAG TGTTTCGTGT ATATCACTGC 
AACTGGCTTC TCCCAGGTCA GAAGGGGAAT GTGGAAGCCT GCATCAAAGT GCTGAGTGAC 
GTGGCCAAAG GCAGAGCCAT AGCCATTCCT GTGGATCTGG ACTGTCAGGT GAATAATCTG 
TTCATGAAGT CCAACAACAT TGTGCAGAAA ACAGCCCTCA GCTGGAGACT CTCTGTCCGT 
AACGCGACTC GGAGAGACTC TGTTCTGACC ACCTCTCGAG ACTATCGCAA CATCATTGAA 
AGACTACAGG ATATAGTGTC TGCATTGGAG GAGCGGTTGA GGCCTCTGGT TCAGGCTGAG 
TTGTCAGTGT TGGTGGATGT TCTCCATCGC CCAGAGCTGC TGTTTCCAGA GCACACTGAA 
GCCCACCGCA AGTGTGAGAG TGGAGGCTTC ATCTGCAAGT TGATAAAACA CACTAAGCAG 
CTTCTAGAAG AAAACGAAGA GAGGCTTTGC ATCAAAGTCC TTCAGACACT CCGAGAGATG 
ATGACCAAAG ACAGAGGATA TGGAGAGAAG GGGGAGGCCC TTCGGCAGCT GCTCGTCAAT 
CGTTACTATG GCTTCAGGAG TGGTGGGCGG AGAGAAAGTC TGACCTCGTT TGGTAACTCC 
ACCCTAACTC CTGTCGGGCC AATCAAGAGC CAGCCAGGGG GGTTGAGCAG GGCTGAGATG 
TCATTAATGG AGGTGCAGTG TCATCTAGAC CGTGAAGGGG CATCGGATCT TGTCATTGAT 
CTCATCATGA ACACTACCAG CGACCGCGTC TTCCATGAAA GCATTCTGTT GGCCATCGCA 
CTGTTGGAGG GCGGAAACAC CATCATACAG CATTCCTTCT TCAAACGTTT AACAGAAGAC 
AAAAATTCAG AGAAGTTTTT CAGGGTGTTT TATGACAGAA TGAAAGTGGC TCAGGTGGAG 
ATCAAAGCCA CGGTTACTGT CAACACTAGT GACCTCGGCA ACAAGCGGCG TGACGACAAC 
ACTCCAGACA AAGATACTCC ACAACGCCGC AGAGGTAAAG ACTCCGGTGT GGTGGTGACA 
GATGATGCTC GTGAGCAGCT GTTGGAGGCT TCAGCTGCGA CTAAGAAGGC CTTTGGTTCA 
TATCGTCGAG ATGCTGATCC CGAGGAGACG TTTGGCACGG CTGATGGAGA TAAAGGAGGT 
GGAGATAAAG GCACAGAGCA GGGCGAGATG AGCCCTGTCA TCCTCATAAT GCAGCCAATC 
CTGCGCTTCC TGCAGCTGCT GTGCGAAAAC CACAATCGAG ACCTGCAGAA TTTTCTGCGC 
TGTCAGAACA ATAAAAACAA CTATAATCTG GTGTGTGAGA CGCTGCAGTT CCTGGACTGT 
ATCTGTGGCA GCACAACAGG AGGCCTGGGG CTGTTAGGAC TCTACATCAA CCAGCATAAC 
GTAGCGCTCA TTAATCAGAC TGTAGAGAGC CTCACTGAAT ACTGTCAAGG TCCCTGCCAT 
GACAATCAGA ATTGCATTGC CACCCATGAG TCTAATGGCA TCGACATCAT TATTGCACTG 
ATTCTGAATG ATATTAATCC ACTGGGCAGG AAGAGAATGG ATCTGGTTCT AGAACTAAAG 
AATAATGCAT CTAAGCTGCT GCTGGCCATC ATGGAGAGCC GTCATGACAG TGAGAATGCA 
GAAAGAATTC TGTACAACAT GAGACCAAAA GAACTAGTGG AGGTGATGAA AATGGCCTAC 
CAGCAGGGGG AGGCAGAGTT TGAGGATGAG GAACAGGAGA ATGGAGAGGA TCATGCAGCA 
TCGCCTCGCA ATGTTGGACA CAACATCTAT ATTCTTGCAC ATCAGCTGTC GCGTCACAAT 
AAGGAATTGC AAATACTACT GAAACCAGGT GGAGAGGACC AGGCCCTGGA GTATTACACA 
AAACACACCT CACAGATAGA GATAGTTCGA CAGGATCGCA CTATGGAACA GATTGTGTTT 
CCTGTACCAA ATATCTGCTC TTTTCTGACA AATGAGTCCA AGCTGCGTGT GTACTATGGT 
ACAGAGAGAG ACGAGCAAGG CAGTAAAATA AATGACTTCT TCTTGCATGC AGACGACCTC 
TTTAATGAAA TGAGGTGGCA GAAAAAACTA AGAGCTCAGC CAGTGCTGTA CTGGTGCAGC 
AGGAACATGT CGTTTTGGAG TAACGTTTCC TTTAATCTGG CGGTGCTCAT TAATTTGCTG 
GTGGCATTTT TCTACCCTCT GGATGGAGTG AGCGAAAGCC AGCTGGAACC TTCTCTGTCT 
CTGTTGCTGT GGGTTTGTTT GTTGGGCTCA CTGGGCTTCG TGTTGATGTC CCCGAGGCCA 
AATGCAGTGC GGGTGCTGGT CATCTCCACT GTCCTGCAGT TGGGCTTCTC TGTAGGGCTG 
CAGCACATGC TCACACTCCT GGGAGCTTTC AATGTTTGTA ATAAGATCGT GTTCATGCTG 
AGCTTTGTGG GTAACCGTGG GTCATTCACC CGGGGTTATC GGGTGATGGT CATGGATAGA 
GAGTTCCTGT TTCATCTGCT GTACCTTCTC ATCTGCATAC TGGGCCTTTT TGGACACGTC 
TTCTTTTACA GTCTTCTGTT GTTTGACCTG GTGAACCGGG AAGAGACTCT TCTGAACGTC 
ATCAAGAGTG TGACTCGAAA CGGCCGCTCC ATCGTCTTGA CCGCAGTGCT GGGCCTCATC 
CTGGTCTACC TCTTTTCTAT TGTTGGATAT ATGTTTTTCA AAGATGACTT CATCCTGGAG 
GTGAACCGCA TCTCCAACGG CACTCTGGAG GAGGGGGTGA CTTTACCTGC TGGTTTTTGT 
GATGCGAGAA ACGGGTCGTG TCTTTCTGAT GATGTTGACG AGGATGACGT GGAGCGCGCG 
TGTGATTCTC TATGGATGTG TATGATCACT GTACTCAGTC ATGGCCTCAG GAGCGGTGGA 
GGAGTTGGAG ATGTCCTGCG CAAACCGTCT AAAGAGGAGC GTCTGTTCGC CGCACGGGTG 
GTGTACGATC TCCTGTTCTT CTTCCTGGTG ATCATCATTG TGTTGAATCT GATCTTCGGT 
GTGATCATTG ACACATTTGC AGATCTGAGA AGCGAGAAGC AGAGAAAAGA GGAGATCTTG 
AAGACCACAT GCTTCATCTG TGGTCTGGAG AGAGATAAGT TTGATAATAA AACGGTGACG 
TTTGAAGAGC ACATCAAGGA GGAGCACAAC CTGTGGCATT ACCTCTTCTT CATAGTGCTG 
GTGCGGGTCA AAGACTCCAC CGAATACACG GGACCCGAGA GCTACGTCGC TCAGATGATC 
AAGGAGCACA ATCTGGACTG GTTTCCTCGT ATGAGGGCGA TGTCTCTGGT CAGCAGTGAT 
GGAGAAGGTG AACAGAATGA ACTGCGCAGC TTACAGGAGA AACTCGAGTC CACAATGAGG 
CTCGTCTCTA ATCTTACCAA TCAGCTCACA GAGCTCAAAG AGCAGATGAC TGAGCAAAGG 
AAACACAAGC AGAGGCTCGG TCTGCTGGGA AACCCCGCCC ACCTCAACAT CAACCCACAG 
CAGCCAGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itpr1a ( XM_009302588.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300863.1 Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X5, mRNA.

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CloneID	ODa45195
Clone ID Related Accession (Same CDS sequence)	XM_009302588.3 , XM_009302588.2
Accession Version	XM_009302588.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8052bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302588.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GGTAAATCCA 
GACTATGAAG AAGAATGCCT GGAGTCCCGC TCCTCTATGG ATTCGGAGCA TGAAGTGATT 
CGGGCTCGTG CACGTAACCC TCAGGATAAG GTGATGTACA CACTGGTGTC TGTTCCTGAT 
GGAAATGACA TATCCTCCAT CTTTGAGCTT GACCCTACAA CTTTGAGGGG CGGAGACTCA 
CTTGTGCCAA GAAACTCGTA TGTGAGGCTC AGGCATCTCT GCACCAACAC GTGGGTCCAC 
AGCACCAACC AGCCAATAGA TAAAGAGGAA GAGAAACCGG TCATGCTCAG AATAGGAACG 
TCTCCTCTGA AGGAAGATAA AGAGGCGTTT GCTATAGTAC CAGTCTCTCC AGCTGAGGTT 
CGAGATCTGG ATTTTGCTAA TGATGCTAGT AAAGTGTTGG CTTCCATTGC TGCCAAACTG 
GAGAAGGGCA CCATTACCCA GAATGAGAGG AGGTCAGTGA CGAAGCTGTT GGAGGATCTG 
GTGTATTTTG TGGTGGACAT ACCCAGCAGT GCTCAAGATG TGCTGGAGAT CACAGTCAAC 
AAACCCAACA GAGAGCGACA GAAACTCATG AGAGAGCAGA ACATTCTTAA ACAGATCTTC 
AAGCTGCTGC AGGCGCCATT TACAGACAGC GGGGACGGCC CTATGTTGCG ATTAGAAGAG 
CTCGCAGACC AGCGTCATGC ACCCTTTCGA CACATTTGCA GACTCTGTTA CAGAGTCCTA 
CGTTACTCAC AACAGGACTA TCGCAAAAAT CAGGAGTACA TAGCAAAGCA GTTCCGCTTC 
ATGCAGAAGC AGATAGGATA TGATGTGCTG GCTGAAGACA CAATTACAGC CCTTCTGCAC 
AACAACAGGA AGCTCCTAGA GAAACACATC ACAGCAGCTG AAATAGATAC CTTTGTCAGT 
CTGGTCCGCA AGAACAGAGA GCCCAGATTT CTGGATTACT TGTCAGATCT GTGTGTGTCG 
ATGAATAAAT CCATCCCGGT CACTCAAGAG CTCATCTGTA ATGCTGTGCT GGACCCAGCC 
AATGCAGACA TTCTCATCGA AACCAAGCTT GTGTTGTCAC GGTTTGAGGT GGCAGGAACG 
GTGTTGGGTG AAGGTGCGGA GGAGGAAGAG GAGGATGAAG AGGAGGTCTG GCTGTTCTGG 
AAGGACAGTG GAGGAGAGGT GAAGAGTAAA AGCATACGAG AGCTTGCACA GGACGCAAAA 
GATGGACATA CAGAGGACCA GGAAGTCATC AATTATTACA GATATCAGCT GAATTTGTTT 
GCTCGGATGT GTCTGGATCG ACAGTATCTT GCGATTAACA AGATATCAGC ACAGCTGGAT 
GTGGATCTGA TTCTGCGCTG CATGTCAGAT GAAGATTTGC CCTTTGACCT CAGAGCATCG 
TTCTGCCGCA TGATGCTGCA CATGCATGTA GACCGTGACC CTCAGGAGCA GGTCACCCCG 
GTCAAATATG CTCGACTGTG GTCTGAAATC CCATCACAAA TTTCCATTGA TGATTATGAT 
AATGATGGGA CGTCAAGTGA TGAAGTGAAA GAGCGCTTCT CTCAGACGAT GGAGTTTGTG 
GAGAATTACC TGAGGGATGT TGTCTGCCAG AGTTTCCCGT TCTCTGATAA GGAGAAAAAC 
AAACTCACTT TTGAGGTGGT GAACCTGGCA AGAAACCTGA TCTATTTTGG TTTCTATAAC 
TTCAGTGATC TGCTAAGGTT AACAAAAATC CTATTGGCAA TCCTGGACTG TGTCCACATA 
AGCACTCCCT TCCACATCAA GCTGGATAGA GAACCCGGTA AAGGAAGTAA CGTGATGCGC 
TCCATCCATG GGGTGGGAGA GCTGATGACA CAGGTGGTGT TGAGGGGTGG GGGATTTCTG 
CCAGCCTCCA GCCACAATCC TCCTGACAGA GACGAAGTGA AAGCACAAAG TGAACCTCAG 
AAACAGGACA TACTGGTGAT GGACACTAAA CTCAAGATCA TCGAGATCCT GCAGTTTATT 
CTGAATGTGC GGTTGGACTA CAGGATCTCC TGCCTCCTCT GTATCTTTAA GACAGAGTTT 
GATGAAAGTA ACTCTCAAAG TGAACCATCT TTAAGCCCAG AATCTCCTGC CAGCGTTCAA 
GGTGCGCTAG ACTTTGAGCA CATTGAAGAG CAGGCGGAGG GTATTTTTGG AGGAAGTAGT 
GAAGAGAACA CCCCGCTGGA TCTTGATGAT CATGGTGGAC GGACATTTCT GAGGGTCCTG 
CTTCACCTGA CCATGCATGA CTATCCTCCG CTGGTATCTC GAGCTCTTCA CCTGCTCTTC 
AGACACTTTA GCCAACGCCA GGAGGTCCTA CAGGCCTTCA AACAGGTTCA GCTCCTGGTG 
ACCAGTCAGG ATGTGGAAAA CTATAAACAG ATCAAGTCAG ATTTGGACCA GCTGAGGTCA 
ATAGTAGAGA AATCAGAGTT GTGGGTGTAT AAGAGACAAG GCTCAGACTC AGGACTGGAT 
GCTGGAGAAG TCACCCCTGA AGCCCACCAC AAGGGTGTCA TCAACTCGAG TAGATCAAAC 
AAACCCAAAG TGGAAAGTAC CAGCAGCTCG AACTACAGAG TCGTGAAAGA GATCCTGCTG 
AGGCTGAGCA AACTGTGTGT TTTGGAGGGC ATCTCTGGCA AGAAGAATAA GAAACAGCAG 
CAGCGGCTCC TCAGGAACAT GGGAGCTCAC AGTGTGGTCC TCGAACTGTT GCAAATCCCT 
TATGAGAAGG GGGAGGATGT CCAGATGCAG GAGATCATGA CCTTAGCACA TCAGTTCCTT 
CAAAACTTCT GTGCTGGAAA TCAGCAGAAC CAGGCACTTC TACACAAACA CATCAACCTG 
TTCCTCAACC CTGGGATCCT GGAGGCGGTG ACCATGCAGC ATATCTTCAT GAATAATTTC 
CAGTTGTGTA GTGAGATTAA TGAGCGGGTC GTGCAGCATT TTGTGCACTG CATCGAGACA 
CACGGCCGCA GCGTGCACTA CCTCAAGTTC CTGCAGACCA TTGTCAAGGC AGAGAACAAG 
TTCATCAAGA AATGCCAAGA CATCGTCATG GCTGAGCTGG TGACTGCAGG TGAGGATGTG 
CTGGTGTTCT ATAATGACCG GGCGTCTTTT CAGTCATTGG TTCAGATGAT GCGTCTTGAG 
CGGGAACGAC TTGATGAGAG CAGTGCACTC AGGTATCACA TTCACCTGGT GGAGTTGCTG 
GCTGTGTGCA CTGAAGGCAA GAACGTCTAC ACTGAGATCA AATGCAACTC GCTGCTCCCT 
CTCGATGATA TTGTACGTGT GGTCACACAT GAGGACTGCA TCCCTGAGGT GAAGATGGCC 
TACGTGAACT TCCTGAATCA CTGTTATGTT GATACAGAGG TTGAGATGAA GGAGATCTAC 
ACCAGCAATC ACATGTGGAA ACTTTTTGAT GATTTTCTGG TGGACGTCTG CAGAGTGTGC 
AATAACACAA GTGACCGTAA ACACGCTGAC ACAGTTCTGG AGAGATACGT TACAGAGACC 
ATAATGAGCA TCGTCACCAC CTTTTTCAGC TCACCATTCT CAGACCAGAG CACAAGTTTG 
CAGACCCGGC AGCCGGTGTT TGTGCAGCTG CTGCAGGGAG TGTTTCGTGT ATATCACTGC 
AACTGGCTTC TCCCAGGTCA GAAGGGGAAT GTGGAAGCCT GCATCAAAGT GCTGAGTGAC 
GTGGCCAAAG GCAGAGCCAT AGCCATTCCT GTGGATCTGG ACTGTCAGGT GAATAATCTG 
TTCATGAAGT CCAACAACAT TGTGCAGAAA ACAGCCCTCA GCTGGAGACT CTCTGTCCGT 
AACGCGACTC GGAGAGACTC TGTTCTGACC ACCTCTCGAG ACTATCGCAA CATCATTGAA 
AGACTACAGG ATATAGTGTC TGCATTGGAG GAGCGGTTGA GGCCTCTGGT TCAGGCTGAG 
TTGTCAGTGT TGGTGGATGT TCTCCATCGC CCAGAGCTGC TGTTTCCAGA GCACACTGAA 
GCCCACCGCA AGTGTGAGAG TGGAGGCTTC ATCTGCAAGT TGATAAAACA CACTAAGCAG 
CTTCTAGAAG AAAACGAAGA GAGGCTTTGC ATCAAAGTCC TTCAGACACT CCGAGAGATG 
ATGACCAAAG ACAGAGGATA TGGAGAGAAG GGGGAGGCCC TTCGGCAGCT GCTCGTCAAT 
CGTTACTATG GCTTCAGGAG TGGTGGGCGG AGAGAAAGTC TGACCTCGTT TGGTAACTCC 
ACCCTAACTC CTGTCGGGCC AATCAAGAGC CAGCCAGGGG GGTTGAGCAG GGCTGAGATG 
TCATTAATGG AGGTGCAGTG TCATCTAGAC CGTGAAGGGG CATCGGATCT TGTCATTGAT 
CTCATCATGA ACACTACCAG CGACCGCGTC TTCCATGAAA GCATTCTGTT GGCCATCGCA 
CTGTTGGAGG GCGGAAACAC CATCATACAG CATTCCTTCT TCAAACGTTT AACAGAAGAC 
AAAAATTCAG AGAAGTTTTT CAGGGTGTTT TATGACAGAA TGAAAGTGGC TCAGGTGGAG 
ATCAAAGCCA CGGTTACTGT CAACACTAGT GACCTCGGCA ACAAGCGGCG TGACGACAAC 
ACTCCAGACA AAGATACTCC ACAACGCCGC AGAGGTAAAG ACTCCGGTGT GGTGGTGACA 
GATGATGCTC GTGAGCAGCT GTTGGAGGCT TCAGCTGCGA CTAAGAAGGC CTTTGGTTCA 
TATCGTCGAG ATGCTGATCC CGAGGAGACG TTTGGCACGG CTGATGGAGA TAAAGGAGGT 
GGAGATAAAG GCACAGAGCA GGGCGAGATG AGCCCTGTCA TCCTCATAAT GCAGCCAATC 
CTGCGCTTCC TGCAGCTGCT GTGCGAAAAC CACAATCGAG ACCTGCAGAA TTTTCTGCGC 
TGTCAGAACA ATAAAAACAA CTATAATCTG GTGTGTGAGA CGCTGCAGTT CCTGGACTGT 
ATCTGTGGCA GCACAACAGG AGGCCTGGGG CTGTTAGGAC TCTACATCAA CCAGCATAAC 
GTAGCGCTCA TTAATCAGAC TGTAGAGAGC CTCACTGAAT ACTGTCAAGG TCCCTGCCAT 
GACAATCAGA ATTGCATTGC CACCCATGAG TCTAATGGCA TCGACATCAT TATTGCACTG 
ATTCTGAATG ATATTAATCC ACTGGGCAGG AAGAGAATGG ATCTGGTTCT AGAACTAAAG 
AATAATGCAT CTAAGCTGCT GCTGGCCATC ATGGAGAGCC GTCATGACAG TGAGAATGCA 
GAAAGAATTC TGTACAACAT GAGACCAAAA GAACTAGTGG AGGTGATGAA AATGGCCTAC 
CAGCAGGGGG AGGCAGAGTT TGAGGATGAG GAACAGGAGA ATGGAGAGGA TCATGCAGCA 
TCGCCTCGCA ATGTTGGACA CAACATCTAT ATTCTTGCAC ATCAGCTGTC GCGTCACAAT 
AAGGAATTGC AAATACTACT GAAACCAGGT GGAGAGGACC AGGCCCTGGA GTATTACACA 
AAACACACCT CACAGATAGA GATAGTTCGA CAGGATCGCA CTATGGAACA GATTGTGTTT 
CCTGTACCAA ATATCTGCTC TTTTCTGACA AATGAGTCCA AGCTGCGTGT GTACTATGGT 
ACAGAGAGAG ACGAGCAAGG CAGTAAAATA AATGACTTCT TCTTGCATGC AGACGACCTC 
TTTAATGAAA TGAGGTGGCA GAAAAAACTA AGAGCTCAGC CAGTGCTGTA CTGGTGCAGC 
AGGAACATGT CGTTTTGGAG TAACGTTTCC TTTAATCTGG CGGTGCTCAT TAATTTGCTG 
GTGGCATTTT TCTACCCTCT GGATGGAGTG AGCGAAAGCC AGCTGGAACC TTCTCTGTCT 
CTGTTGCTGT GGGTTTGTTT GTTGGGCTCA CTGGGCTTCG TGTTGATGTC CCCGAGGCCA 
AATGCAGTGC GGGTGCTGGT CATCTCCACT GTCCTGCAGT TGGGCTTCTC TGTAGGGCTG 
CAGCACATGC TCACACTCCT GGGAGCTTTC AATGTTTGTA ATAAGATCGT GTTCATGCTG 
AGCTTTGTGG GTAACCGTGG GTCATTCACC CGGGGTTATC GGGTGATGGT CATGGATAGA 
GAGTTCCTGT TTCATCTGCT GTACCTTCTC ATCTGCATAC TGGGCCTTTT TGGACACGTC 
TTCTTTTACA GTCTTCTGTT GTTTGACCTG GTGAACCGGG AAGAGACTCT TCTGAACGTC 
ATCAAGAGTG TGACTCGAAA CGGCCGCTCC ATCGTCTTGA CCGCAGTGCT GGGCCTCATC 
CTGGTCTACC TCTTTTCTAT TGTTGGATAT ATGTTTTTCA AAGATGACTT CATCCTGGAG 
GTGAACCGCA TCTCCAACGG CACTCTGGAG GAGGGGGTGA CTTTACCTGC TGGTTTTTGT 
GATGCGAGAA ACGGGTCGTG TCTTTCTGAT GATGTTGACG AGGATGACGT GGAGCGCGCG 
TGTGATTCTC TATGGATGTG TATGATCACT GTACTCAGTC ATGGCCTCAG GAGCGGTGGA 
GGAGTTGGAG ATGTCCTGCG CAAACCGTCT AAAGAGGAGC GTCTGTTCGC CGCACGGGTG 
GTGTACGATC TCCTGTTCTT CTTCCTGGTG ATCATCATTG TGTTGAATCT GATCTTCGGT 
GTGATCATTG ACACATTTGC AGATCTGAGA AGCGAGAAGC AGAGAAAAGA GGAGATCTTG 
AAGACCACAT GCTTCATCTG TGGTCTGGAG AGAGATAAGT TTGATAATAA AACGGTGACG 
TTTGAAGAGC ACATCAAGGA GGAGCACAAC CTGTGGCATT ACCTCTTCTT CATAGTGCTG 
GTGCGGGTCA AAGACTCCAC CGAATACACG GGACCCGAGA GCTACGTCGC TCAGATGATC 
AAGGAGCACA ATCTGGACTG GTTTCCTCGT ATGAGGGCGA TGTCTCTGGT CAGCAGTGAT 
GGAGAAGGTG AACAGAATGA ACTGCGCAGC TTACAGGAGA AACTCGAGTC CACAATGAGG 
CTCGTCTCTA ATCTTACCAA TCAGCTCACA GAGCTCAAAG AGCAGATGAC TGAGCAAAGG 
AAACACAAGC AGAGGCTCGG TCTGCTGGGA AACCCCGCCC ACCTCAACAT CAACCCACAG 
CAGCCAGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300863.1
CDS	148..8199
Translation	MVDKLSSFLHIGDVCSLYAEGSTSGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGTSTTDTVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDAAGNEGSWFYIQPFYKLRSIGDNVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCTTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDEHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEVNPDYEEECLESRSSMDSEHEVIRARARNPQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSPLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAAKLEKGTITQNERRSVTKLLEDLVYFVVDIPSSAQDVLEITVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRYSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDPANADILIETKLVLSRFEVAGTVLGEGAEEEEEDEEEVWLFWKDSGGEVKSKSIRELAQDAKDGHTEDQEVINYYRYQLNLFARMCLDRQYLAINKISAQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSQISIDDYDNDGTSSDEVKERFSQTMEFVENYLRDVVCQSFPFSDKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHISTPFHIKLDREPGKGSNVMRSIHGVGELMTQVVLRGGGFLPASSHNPPDRDEVKAQSEPQKQDILVMDTKLKIIEILQFILNVRLDYRISCLLCIFKTEFDESNSQSEPSLSPESPASVQGALDFEHIEEQAEGIFGGSSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALHLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSDSGLDAGEVTPEAHHKGVINSSRSNKPKVESTSSSNYRVVKEILLRLSKLCVLEGISGKKNKKQQQRLLRNMGAHSVVLELLQIPYEKGEDVQMQEIMTLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAVTMQHIFMNNFQLCSEINERVVQHFVHCIETHGRSVHYLKFLQTIVKAENKFIKKCQDIVMAELVTAGEDVLVFYNDRASFQSLVQMMRLERERLDESSALRYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKMAYVNFLNHCYVDTEVEMKEIYTSNHMWKLFDDFLVDVCRVCNNTSDRKHADTVLERYVTETIMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQGVFRVYHCNWLLPGQKGNVEACIKVLSDVAKGRAIAIPVDLDCQVNNLFMKSNNIVQKTALSWRLSVRNATRRDSVLTTSRDYRNIIERLQDIVSALEERLRPLVQAELSVLVDVLHRPELLFPEHTEAHRKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKGEALRQLLVNRYYGFRSGGRRESLTSFGNSTLTPVGPIKSQPGGLSRAEMSLMEVQCHLDREGASDLVIDLIMNTTSDRVFHESILLAIALLEGGNTIIQHSFFKRLTEDKNSEKFFRVFYDRMKVAQVEIKATVTVNTSDLGNKRRDDNTPDKDTPQRRRGKDSGVVVTDDAREQLLEASAATKKAFGSYRRDADPEETFGTADGDKGGGDKGTEQGEMSPVILIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINQHNVALINQTVESLTEYCQGPCHDNQNCIATHESNGIDIIIALILNDINPLGRKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVMKMAYQQGEAEFEDEEQENGEDHAASPRNVGHNIYILAHQLSRHNKELQILLKPGGEDQALEYYTKHTSQIEIVRQDRTMEQIVFPVPNICSFLTNESKLRVYYGTERDEQGSKINDFFLHADDLFNEMRWQKKLRAQPVLYWCSRNMSFWSNVSFNLAVLINLLVAFFYPLDGVSESQLEPSLSLLLWVCLLGSLGFVLMSPRPNAVRVLVISTVLQLGFSVGLQHMLTLLGAFNVCNKIVFMLSFVGNRGSFTRGYRVMVMDREFLFHLLYLLICILGLFGHVFFYSLLLFDLVNREETLLNVIKSVTRNGRSIVLTAVLGLILVYLFSIVGYMFFKDDFILEVNRISNGTLEEGVTLPAGFCDARNGSCLSDDVDEDDVERACDSLWMCMITVLSHGLRSGGGVGDVLRKPSKEERLFAARVVYDLLFFFLVIIIVLNLIFGVIIDTFADLRSEKQRKEEILKTTCFICGLERDKFDNKTVTFEEHIKEEHNLWHYLFFIVLVRVKDSTEYTGPESYVAQMIKEHNLDWFPRMRAMSLVSSDGEGEQNELRSLQEKLESTMRLVSNLTNQLTELKEQMTEQRKHKQRLGLLGNPAHLNINPQQPA

Target ORF information:

RefSeq Version	XM_009302588.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302588.2

ORF Insert Sequence:

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GGTAAATCCA 
GACTATGAAG AAGAATGCCT GGAGTCCCGC TCCTCTATGG ATTCGGAGCA TGAAGTGATT 
CGGGCTCGTG CACGTAACCC TCAGGATAAG GTGATGTACA CACTGGTGTC TGTTCCTGAT 
GGAAATGACA TATCCTCCAT CTTTGAGCTT GACCCTACAA CTTTGAGGGG CGGAGACTCA 
CTTGTGCCAA GAAACTCGTA TGTGAGGCTC AGGCATCTCT GCACCAACAC GTGGGTCCAC 
AGCACCAACC AGCCAATAGA TAAAGAGGAA GAGAAACCGG TCATGCTCAG AATAGGAACG 
TCTCCTCTGA AGGAAGATAA AGAGGCGTTT GCTATAGTAC CAGTCTCTCC AGCTGAGGTT 
CGAGATCTGG ATTTTGCTAA TGATGCTAGT AAAGTGTTGG CTTCCATTGC TGCCAAACTG 
GAGAAGGGCA CCATTACCCA GAATGAGAGG AGGTCAGTGA CGAAGCTGTT GGAGGATCTG 
GTGTATTTTG TGGTGGACAT ACCCAGCAGT GCTCAAGATG TGCTGGAGAT CACAGTCAAC 
AAACCCAACA GAGAGCGACA GAAACTCATG AGAGAGCAGA ACATTCTTAA ACAGATCTTC 
AAGCTGCTGC AGGCGCCATT TACAGACAGC GGGGACGGCC CTATGTTGCG ATTAGAAGAG 
CTCGCAGACC AGCGTCATGC ACCCTTTCGA CACATTTGCA GACTCTGTTA CAGAGTCCTA 
CGTTACTCAC AACAGGACTA TCGCAAAAAT CAGGAGTACA TAGCAAAGCA GTTCCGCTTC 
ATGCAGAAGC AGATAGGATA TGATGTGCTG GCTGAAGACA CAATTACAGC CCTTCTGCAC 
AACAACAGGA AGCTCCTAGA GAAACACATC ACAGCAGCTG AAATAGATAC CTTTGTCAGT 
CTGGTCCGCA AGAACAGAGA GCCCAGATTT CTGGATTACT TGTCAGATCT GTGTGTGTCG 
ATGAATAAAT CCATCCCGGT CACTCAAGAG CTCATCTGTA ATGCTGTGCT GGACCCAGCC 
AATGCAGACA TTCTCATCGA AACCAAGCTT GTGTTGTCAC GGTTTGAGGT GGCAGGAACG 
GTGTTGGGTG AAGGTGCGGA GGAGGAAGAG GAGGATGAAG AGGAGGTCTG GCTGTTCTGG 
AAGGACAGTG GAGGAGAGGT GAAGAGTAAA AGCATACGAG AGCTTGCACA GGACGCAAAA 
GATGGACATA CAGAGGACCA GGAAGTCATC AATTATTACA GATATCAGCT GAATTTGTTT 
GCTCGGATGT GTCTGGATCG ACAGTATCTT GCGATTAACA AGATATCAGC ACAGCTGGAT 
GTGGATCTGA TTCTGCGCTG CATGTCAGAT GAAGATTTGC CCTTTGACCT CAGAGCATCG 
TTCTGCCGCA TGATGCTGCA CATGCATGTA GACCGTGACC CTCAGGAGCA GGTCACCCCG 
GTCAAATATG CTCGACTGTG GTCTGAAATC CCATCACAAA TTTCCATTGA TGATTATGAT 
AATGATGGGA CGTCAAGTGA TGAAGTGAAA GAGCGCTTCT CTCAGACGAT GGAGTTTGTG 
GAGAATTACC TGAGGGATGT TGTCTGCCAG AGTTTCCCGT TCTCTGATAA GGAGAAAAAC 
AAACTCACTT TTGAGGTGGT GAACCTGGCA AGAAACCTGA TCTATTTTGG TTTCTATAAC 
TTCAGTGATC TGCTAAGGTT AACAAAAATC CTATTGGCAA TCCTGGACTG TGTCCACATA 
AGCACTCCCT TCCACATCAA GCTGGATAGA GAACCCGGTA AAGGAAGTAA CGTGATGCGC 
TCCATCCATG GGGTGGGAGA GCTGATGACA CAGGTGGTGT TGAGGGGTGG GGGATTTCTG 
CCAGCCTCCA GCCACAATCC TCCTGACAGA GACGAAGTGA AAGCACAAAG TGAACCTCAG 
AAACAGGACA TACTGGTGAT GGACACTAAA CTCAAGATCA TCGAGATCCT GCAGTTTATT 
CTGAATGTGC GGTTGGACTA CAGGATCTCC TGCCTCCTCT GTATCTTTAA GACAGAGTTT 
GATGAAAGTA ACTCTCAAAG TGAACCATCT TTAAGCCCAG AATCTCCTGC CAGCGTTCAA 
GGTGCGCTAG ACTTTGAGCA CATTGAAGAG CAGGCGGAGG GTATTTTTGG AGGAAGTAGT 
GAAGAGAACA CCCCGCTGGA TCTTGATGAT CATGGTGGAC GGACATTTCT GAGGGTCCTG 
CTTCACCTGA CCATGCATGA CTATCCTCCG CTGGTATCTC GAGCTCTTCA CCTGCTCTTC 
AGACACTTTA GCCAACGCCA GGAGGTCCTA CAGGCCTTCA AACAGGTTCA GCTCCTGGTG 
ACCAGTCAGG ATGTGGAAAA CTATAAACAG ATCAAGTCAG ATTTGGACCA GCTGAGGTCA 
ATAGTAGAGA AATCAGAGTT GTGGGTGTAT AAGAGACAAG GCTCAGACTC AGGACTGGAT 
GCTGGAGAAG TCACCCCTGA AGCCCACCAC AAGGGTGTCA TCAACTCGAG TAGATCAAAC 
AAACCCAAAG TGGAAAGTAC CAGCAGCTCG AACTACAGAG TCGTGAAAGA GATCCTGCTG 
AGGCTGAGCA AACTGTGTGT TTTGGAGGGC ATCTCTGGCA AGAAGAATAA GAAACAGCAG 
CAGCGGCTCC TCAGGAACAT GGGAGCTCAC AGTGTGGTCC TCGAACTGTT GCAAATCCCT 
TATGAGAAGG GGGAGGATGT CCAGATGCAG GAGATCATGA CCTTAGCACA TCAGTTCCTT 
CAAAACTTCT GTGCTGGAAA TCAGCAGAAC CAGGCACTTC TACACAAACA CATCAACCTG 
TTCCTCAACC CTGGGATCCT GGAGGCGGTG ACCATGCAGC ATATCTTCAT GAATAATTTC 
CAGTTGTGTA GTGAGATTAA TGAGCGGGTC GTGCAGCATT TTGTGCACTG CATCGAGACA 
CACGGCCGCA GCGTGCACTA CCTCAAGTTC CTGCAGACCA TTGTCAAGGC AGAGAACAAG 
TTCATCAAGA AATGCCAAGA CATCGTCATG GCTGAGCTGG TGACTGCAGG TGAGGATGTG 
CTGGTGTTCT ATAATGACCG GGCGTCTTTT CAGTCATTGG TTCAGATGAT GCGTCTTGAG 
CGGGAACGAC TTGATGAGAG CAGTGCACTC AGGTATCACA TTCACCTGGT GGAGTTGCTG 
GCTGTGTGCA CTGAAGGCAA GAACGTCTAC ACTGAGATCA AATGCAACTC GCTGCTCCCT 
CTCGATGATA TTGTACGTGT GGTCACACAT GAGGACTGCA TCCCTGAGGT GAAGATGGCC 
TACGTGAACT TCCTGAATCA CTGTTATGTT GATACAGAGG TTGAGATGAA GGAGATCTAC 
ACCAGCAATC ACATGTGGAA ACTTTTTGAT GATTTTCTGG TGGACGTCTG CAGAGTGTGC 
AATAACACAA GTGACCGTAA ACACGCTGAC ACAGTTCTGG AGAGATACGT TACAGAGACC 
ATAATGAGCA TCGTCACCAC CTTTTTCAGC TCACCATTCT CAGACCAGAG CACAAGTTTG 
CAGACCCGGC AGCCGGTGTT TGTGCAGCTG CTGCAGGGAG TGTTTCGTGT ATATCACTGC 
AACTGGCTTC TCCCAGGTCA GAAGGGGAAT GTGGAAGCCT GCATCAAAGT GCTGAGTGAC 
GTGGCCAAAG GCAGAGCCAT AGCCATTCCT GTGGATCTGG ACTGTCAGGT GAATAATCTG 
TTCATGAAGT CCAACAACAT TGTGCAGAAA ACAGCCCTCA GCTGGAGACT CTCTGTCCGT 
AACGCGACTC GGAGAGACTC TGTTCTGACC ACCTCTCGAG ACTATCGCAA CATCATTGAA 
AGACTACAGG ATATAGTGTC TGCATTGGAG GAGCGGTTGA GGCCTCTGGT TCAGGCTGAG 
TTGTCAGTGT TGGTGGATGT TCTCCATCGC CCAGAGCTGC TGTTTCCAGA GCACACTGAA 
GCCCACCGCA AGTGTGAGAG TGGAGGCTTC ATCTGCAAGT TGATAAAACA CACTAAGCAG 
CTTCTAGAAG AAAACGAAGA GAGGCTTTGC ATCAAAGTCC TTCAGACACT CCGAGAGATG 
ATGACCAAAG ACAGAGGATA TGGAGAGAAG GGGGAGGCCC TTCGGCAGCT GCTCGTCAAT 
CGTTACTATG GCTTCAGGAG TGGTGGGCGG AGAGAAAGTC TGACCTCGTT TGGTAACTCC 
ACCCTAACTC CTGTCGGGCC AATCAAGAGC CAGCCAGGGG GGTTGAGCAG GGCTGAGATG 
TCATTAATGG AGGTGCAGTG TCATCTAGAC CGTGAAGGGG CATCGGATCT TGTCATTGAT 
CTCATCATGA ACACTACCAG CGACCGCGTC TTCCATGAAA GCATTCTGTT GGCCATCGCA 
CTGTTGGAGG GCGGAAACAC CATCATACAG CATTCCTTCT TCAAACGTTT AACAGAAGAC 
AAAAATTCAG AGAAGTTTTT CAGGGTGTTT TATGACAGAA TGAAAGTGGC TCAGGTGGAG 
ATCAAAGCCA CGGTTACTGT CAACACTAGT GACCTCGGCA ACAAGCGGCG TGACGACAAC 
ACTCCAGACA AAGATACTCC ACAACGCCGC AGAGGTAAAG ACTCCGGTGT GGTGGTGACA 
GATGATGCTC GTGAGCAGCT GTTGGAGGCT TCAGCTGCGA CTAAGAAGGC CTTTGGTTCA 
TATCGTCGAG ATGCTGATCC CGAGGAGACG TTTGGCACGG CTGATGGAGA TAAAGGAGGT 
GGAGATAAAG GCACAGAGCA GGGCGAGATG AGCCCTGTCA TCCTCATAAT GCAGCCAATC 
CTGCGCTTCC TGCAGCTGCT GTGCGAAAAC CACAATCGAG ACCTGCAGAA TTTTCTGCGC 
TGTCAGAACA ATAAAAACAA CTATAATCTG GTGTGTGAGA CGCTGCAGTT CCTGGACTGT 
ATCTGTGGCA GCACAACAGG AGGCCTGGGG CTGTTAGGAC TCTACATCAA CCAGCATAAC 
GTAGCGCTCA TTAATCAGAC TGTAGAGAGC CTCACTGAAT ACTGTCAAGG TCCCTGCCAT 
GACAATCAGA ATTGCATTGC CACCCATGAG TCTAATGGCA TCGACATCAT TATTGCACTG 
ATTCTGAATG ATATTAATCC ACTGGGCAGG AAGAGAATGG ATCTGGTTCT AGAACTAAAG 
AATAATGCAT CTAAGCTGCT GCTGGCCATC ATGGAGAGCC GTCATGACAG TGAGAATGCA 
GAAAGAATTC TGTACAACAT GAGACCAAAA GAACTAGTGG AGGTGATGAA AATGGCCTAC 
CAGCAGGGGG AGGCAGAGTT TGAGGATGAG GAACAGGAGA ATGGAGAGGA TCATGCAGCA 
TCGCCTCGCA ATGTTGGACA CAACATCTAT ATTCTTGCAC ATCAGCTGTC GCGTCACAAT 
AAGGAATTGC AAATACTACT GAAACCAGGT GGAGAGGACC AGGCCCTGGA GTATTACACA 
AAACACACCT CACAGATAGA GATAGTTCGA CAGGATCGCA CTATGGAACA GATTGTGTTT 
CCTGTACCAA ATATCTGCTC TTTTCTGACA AATGAGTCCA AGCTGCGTGT GTACTATGGT 
ACAGAGAGAG ACGAGCAAGG CAGTAAAATA AATGACTTCT TCTTGCATGC AGACGACCTC 
TTTAATGAAA TGAGGTGGCA GAAAAAACTA AGAGCTCAGC CAGTGCTGTA CTGGTGCAGC 
AGGAACATGT CGTTTTGGAG TAACGTTTCC TTTAATCTGG CGGTGCTCAT TAATTTGCTG 
GTGGCATTTT TCTACCCTCT GGATGGAGTG AGCGAAAGCC AGCTGGAACC TTCTCTGTCT 
CTGTTGCTGT GGGTTTGTTT GTTGGGCTCA CTGGGCTTCG TGTTGATGTC CCCGAGGCCA 
AATGCAGTGC GGGTGCTGGT CATCTCCACT GTCCTGCAGT TGGGCTTCTC TGTAGGGCTG 
CAGCACATGC TCACACTCCT GGGAGCTTTC AATGTTTGTA ATAAGATCGT GTTCATGCTG 
AGCTTTGTGG GTAACCGTGG GTCATTCACC CGGGGTTATC GGGTGATGGT CATGGATAGA 
GAGTTCCTGT TTCATCTGCT GTACCTTCTC ATCTGCATAC TGGGCCTTTT TGGACACGTC 
TTCTTTTACA GTCTTCTGTT GTTTGACCTG GTGAACCGGG AAGAGACTCT TCTGAACGTC 
ATCAAGAGTG TGACTCGAAA CGGCCGCTCC ATCGTCTTGA CCGCAGTGCT GGGCCTCATC 
CTGGTCTACC TCTTTTCTAT TGTTGGATAT ATGTTTTTCA AAGATGACTT CATCCTGGAG 
GTGAACCGCA TCTCCAACGG CACTCTGGAG GAGGGGGTGA CTTTACCTGC TGGTTTTTGT 
GATGCGAGAA ACGGGTCGTG TCTTTCTGAT GATGTTGACG AGGATGACGT GGAGCGCGCG 
TGTGATTCTC TATGGATGTG TATGATCACT GTACTCAGTC ATGGCCTCAG GAGCGGTGGA 
GGAGTTGGAG ATGTCCTGCG CAAACCGTCT AAAGAGGAGC GTCTGTTCGC CGCACGGGTG 
GTGTACGATC TCCTGTTCTT CTTCCTGGTG ATCATCATTG TGTTGAATCT GATCTTCGGT 
GTGATCATTG ACACATTTGC AGATCTGAGA AGCGAGAAGC AGAGAAAAGA GGAGATCTTG 
AAGACCACAT GCTTCATCTG TGGTCTGGAG AGAGATAAGT TTGATAATAA AACGGTGACG 
TTTGAAGAGC ACATCAAGGA GGAGCACAAC CTGTGGCATT ACCTCTTCTT CATAGTGCTG 
GTGCGGGTCA AAGACTCCAC CGAATACACG GGACCCGAGA GCTACGTCGC TCAGATGATC 
AAGGAGCACA ATCTGGACTG GTTTCCTCGT ATGAGGGCGA TGTCTCTGGT CAGCAGTGAT 
GGAGAAGGTG AACAGAATGA ACTGCGCAGC TTACAGGAGA AACTCGAGTC CACAATGAGG 
CTCGTCTCTA ATCTTACCAA TCAGCTCACA GAGCTCAAAG AGCAGATGAC TGAGCAAAGG 
AAACACAAGC AGAGGCTCGG TCTGCTGGGA AACCCCGCCC ACCTCAACAT CAACCCACAG 
CAGCCAGCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itpr1a ( XM_017356664.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212153.1 Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X4, mRNA.

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CloneID	ODa59493
Clone ID Related Accession (Same CDS sequence)	XM_017356664.2 , XM_017356664.1
Accession Version	XM_017356664.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356664.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GATGGATTCG 
GAGCATGAAG TGATTCGGGC TCGTGCACGT AACCCTCAGG ATAAGGTGAT GTACACACTG 
GTGTCTGTTC CTGATGGAAA TGACATATCC TCCATCTTTG AGCTTGACCC TACAACTTTG 
AGGGGCGGAG ACTCACTTGT GCCAAGAAAC TCGTATGTGA GGCTCAGGCA TCTCTGCACC 
AACACGTGGG TCCACAGCAC CAACCAGCCA ATAGATAAAG AGGAAGAGAA ACCGGTCATG 
CTCAGAATAG GAACGTCTCC TCTGAAGGAA GATAAAGAGG CGTTTGCTAT AGTACCAGTC 
TCTCCAGCTG AGGTTCGAGA TCTGGATTTT GCTAATGATG CTAGTAAAGT GTTGGCTTCC 
ATTGCTGCCA AACTGGAGAA GGGCACCATT ACCCAGAATG AGAGGAGGTC AGTGACGAAG 
CTGTTGGAGG ATCTGGTGTA TTTTGTGGTG GACATACCCA GCAGTGCTCA AGATGTGCTG 
GAGATCACAG TCAACAAACC CAACAGAGAG CGACAGAAAC TCATGAGAGA GCAGAACATT 
CTTAAACAGA TCTTCAAGCT GCTGCAGGCG CCATTTACAG ACAGCGGGGA CGGCCCTATG 
TTGCGATTAG AAGAGCTCGC AGACCAGCGT CATGCACCCT TTCGACACAT TTGCAGACTC 
TGTTACAGAG TCCTACGTTA CTCACAACAG GACTATCGCA AAAATCAGGA GTACATAGCA 
AAGCAGTTCC GCTTCATGCA GAAGCAGATA GGATATGATG TGCTGGCTGA AGACACAATT 
ACAGCCCTTC TGCACAACAA CAGGAAGCTC CTAGAGAAAC ACATCACAGC AGCTGAAATA 
GATACCTTTG TCAGTCTGGT CCGCAAGAAC AGAGAGCCCA GATTTCTGGA TTACTTGTCA 
GATCTGTGTG TGTCGATGAA TAAATCCATC CCGGTCACTC AAGAGCTCAT CTGTAATGCT 
GTGCTGGACC CAGCCAATGC AGACATTCTC ATCGAAACCA AGCTTGTGTT GTCACGGTTT 
GAGGTGGCAG GAACGGTGTT GGGTGAAGGT GCGGAGGAGG AAGAGGAGGA TGAAGAGGAG 
GTCTGGCTGT TCTGGAAGGA CAGTGGAGGA GAGGTGAAGA GTAAAAGCAT ACGAGAGCTT 
GCACAGGACG CAAAAGATGG ACATACAGAG GACCAGGAAG TCATCAATTA TTACAGATAT 
CAGCTGAATT TGTTTGCTCG GATGTGTCTG GATCGACAGT ATCTTGCGAT TAACAAGATA 
TCAGCACAGC TGGATGTGGA TCTGATTCTG CGCTGCATGT CAGATGAAGA TTTGCCCTTT 
GACCTCAGAG CATCGTTCTG CCGCATGATG CTGCACATGC ATGTAGACCG TGACCCTCAG 
GAGCAGGTCA CCCCGGTCAA ATATGCTCGA CTGTGGTCTG AAATCCCATC ACAAATTTCC 
ATTGATGATT ATGATAATGA TGGGACGTCA AGTGATGAAG TGAAAGAGCG CTTCTCTCAG 
ACGATGGAGT TTGTGGAGAA TTACCTGAGG GATGTTGTCT GCCAGAGTTT CCCGTTCTCT 
GATAAGGAGA AAAACAAACT CACTTTTGAG GTGGTGAACC TGGCAAGAAA CCTGATCTAT 
TTTGGTTTCT ATAACTTCAG TGATCTGCTA AGGTTAACAA AAATCCTATT GGCAATCCTG 
GACTGTGTCC ACATAAGCAC TCCCTTCCAC ATCAAGCTGG ATAGAGAACC CGGTAAAGGA 
AGTAACGTGA TGCGCTCCAT CCATGGGGTG GGAGAGCTGA TGACACAGGT GGTGTTGAGG 
GGTGGGGGAT TTCTGCCAGC CTCCAGCCAC AATCCTCCTG ACAGAGACGA AGTGAAAGCA 
CAAAGTGAAC CTCAGAAACA GGACATACTG GTGATGGACA CTAAACTCAA GATCATCGAG 
ATCCTGCAGT TTATTCTGAA TGTGCGGTTG GACTACAGGA TCTCCTGCCT CCTCTGTATC 
TTTAAGACAG AGTTTGATGA AAGTAACTCT CAAAGTGAAC CATCTTTAAG CCCAGAATCT 
CCTGCCAGCG TTCAAGGTGC GCTAGACTTT GAGCACATTG AAGAGCAGGC GGAGGGTATT 
TTTGGAGGAA GTGAAGAGAA CACCCCGCTG GATCTTGATG ATCATGGTGG ACGGACATTT 
CTGAGGGTCC TGCTTCACCT GACCATGCAT GACTATCCTC CGCTGGTATC TCGAGCTCTT 
CACCTGCTCT TCAGACACTT TAGCCAACGC CAGGAGGTCC TACAGGCCTT CAAACAGGTT 
CAGCTCCTGG TGACCAGTCA GGATGTGGAA AACTATAAAC AGATCAAGTC AGATTTGGAC 
CAGCTGAGGT CAATAGTAGA GAAATCAGAG TTGTGGGTGT ATAAGAGACA AGGCTCAGAC 
TCAGGACTGG ATGCTGGAGA AGTCACCCCT GAAGCCCACC ACAAGGGTGT CATCAACTCG 
AGTAGATCAA ACAAACCCAA AGTGGAAAGT ACCAGCAGCT CGAACTACAG AGTCGTGAAA 
GAGATCCTGC TGAGGCTGAG CAAACTGTGT GTTTTGGAGG GCATCTCTGG CAAGAAGAAT 
AAGAAACAGC AGCAGCGGCT CCTCAGGAAC ATGGGAGCTC ACAGTGTGGT CCTCGAACTG 
TTGCAAATCC CTTATGAGAA GGGGGAGGAT GTCCAGATGC AGGAGATCAT GACCTTAGCA 
CATCAGTTCC TTCAAAACTT CTGTGCTGGA AATCAGCAGA ACCAGGCACT TCTACACAAA 
CACATCAACC TGTTCCTCAA CCCTGGGATC CTGGAGGCGG TGACCATGCA GCATATCTTC 
ATGAATAATT TCCAGTTGTG TAGTGAGATT AATGAGCGGG TCGTGCAGCA TTTTGTGCAC 
TGCATCGAGA CACACGGCCG CAGCGTGCAC TACCTCAAGT TCCTGCAGAC CATTGTCAAG 
GCAGAGAACA AGTTCATCAA GAAATGCCAA GACATCGTCA TGGCTGAGCT GGTGACTGCA 
GGTGAGGATG TGCTGGTGTT CTATAATGAC CGGGCGTCTT TTCAGTCATT GGTTCAGATG 
ATGCGTCTTG AGCGGGAACG ACTTGATGAG AGCAGTGCAC TCAGGTATCA CATTCACCTG 
GTGGAGTTGC TGGCTGTGTG CACTGAAGGC AAGAACGTCT ACACTGAGAT CAAATGCAAC 
TCGCTGCTCC CTCTCGATGA TATTGTACGT GTGGTCACAC ATGAGGACTG CATCCCTGAG 
GTGAAGATGG CCTACGTGAA CTTCCTGAAT CACTGTTATG TTGATACAGA GGTTGAGATG 
AAGGAGATCT ACACCAGCAA TCACATGTGG AAACTTTTTG ATGATTTTCT GGTGGACGTC 
TGCAGAGTGT GCAATAACAC AAGTGACCGT AAACACGCTG ACACAGTTCT GGAGAGATAC 
GTTACAGAGA CCATAATGAG CATCGTCACC ACCTTTTTCA GCTCACCATT CTCAGACCAG 
AGCACAAGTT TGCAGACCCG GCAGCCGGTG TTTGTGCAGC TGCTGCAGGG AGTGTTTCGT 
GTATATCACT GCAACTGGCT TCTCCCAGGT CAGAAGGGGA ATGTGGAAGC CTGCATCAAA 
GTGCTGAGTG ACGTGGCCAA AGGCAGAGCC ATAGCCATTC CTGTGGATCT GGACTGTCAG 
GTGAATAATC TGTTCATGAA GTCCAACAAC ATTGTGCAGA AAACAGCCCT CAGCTGGAGA 
CTCTCTGTCC GTAACGCGAC TCGGAGAGAC TCTGTTCTGA CCACCTCTCG AGACTATCGC 
AACATCATTG AAAGACTACA GGATATAGTG TCTGCATTGG AGGAGCGGTT GAGGCCTCTG 
GTTCAGGCTG AGTTGTCAGT GTTGGTGGAT GTTCTCCATC GCCCAGAGCT GCTGTTTCCA 
GAGCACACTG AAGCCCACCG CAAGTGTGAG AGTGGAGGCT TCATCTGCAA GTTGATAAAA 
CACACTAAGC AGCTTCTAGA AGAAAACGAA GAGAGGCTTT GCATCAAAGT CCTTCAGACA 
CTCCGAGAGA TGATGACCAA AGACAGAGGA TATGGAGAGA AGCTTCTGGG ATATGATGAT 
GAGATGGATG TCACGGAGGT GGTGGATATC AGTCTGCCAC CCAAACAGCA GGAGGATCGG 
CAGAGGGGGG AGGCCCTTCG GCAGCTGCTC GTCAATCGTT ACTATGGCTT CAGGAGTGGT 
GGGCGGAGAG AAAGTCTGAC CTCGTTTGGT AACTCCACCC TAACTCCTGT CGGGCCAATC 
AAGAGCCAGC CAGGGGGGTT GAGCAGGGCT GAGATGTCAT TAATGGAGGT GCAGTGTCAT 
CTAGACCGTG AAGGGGCATC GGATCTTGTC ATTGATCTCA TCATGAACAC TACCAGCGAC 
CGCGTCTTCC ATGAAAGCAT TCTGTTGGCC ATCGCACTGT TGGAGGGCGG AAACACCATC 
ATACAGCATT CCTTCTTCAA ACGTTTAACA GAAGACAAAA ATTCAGAGAA GTTTTTCAGG 
GTGTTTTATG ACAGAATGAA AGTGGCTCAG GTGGAGATCA AAGCCACGGT TACTGTCAAC 
ACTAGTGACC TCGGCAACAA GCGGCGTGAC GACAACACTC CAGACAAAGA TACTCCACAA 
CGCCGCAGAG GTAAAGACTC CGGTGTGGTG GTGACAGATG ATGCTCGTGA GCAGCTGTTG 
GAGGCTTCAG CTGCGACTAA GAAGGCCTTT GGTTCATATC GTCGAGATGC TGATCCCGAG 
GAGACGTTTG GCACGGCTGA TGGAGATAAA GGAGGTGGAG ATAAAGGCAC AGAGCAGGGC 
GAGATGAGCC CTGTCATCCT CATAATGCAG CCAATCCTGC GCTTCCTGCA GCTGCTGTGC 
GAAAACCACA ATCGAGACCT GCAGAATTTT CTGCGCTGTC AGAACAATAA AAACAACTAT 
AATCTGGTGT GTGAGACGCT GCAGTTCCTG GACTGTATCT GTGGCAGCAC AACAGGAGGC 
CTGGGGCTGT TAGGACTCTA CATCAACCAG CATAACGTAG CGCTCATTAA TCAGACTGTA 
GAGAGCCTCA CTGAATACTG TCAAGGTCCC TGCCATGACA ATCAGAATTG CATTGCCACC 
CATGAGTCTA ATGGCATCGA CATCATTATT GCACTGATTC TGAATGATAT TAATCCACTG 
GGCAGGAAGA GAATGGATCT GGTTCTAGAA CTAAAGAATA ATGCATCTAA GCTGCTGCTG 
GCCATCATGG AGAGCCGTCA TGACAGTGAG AATGCAGAAA GAATTCTGTA CAACATGAGA 
CCAAAAGAAC TAGTGGAGGT GATGAAAATG GCCTACCAGC AGGGGGAGGC AGAGTTTGAG 
GATGAGGAAC AGGAGAATGG AGAGGATCAT GCAGCATCGC CTCGCAATGT TGGACACAAC 
ATCTATATTC TTGCACATCA GCTGTCGCGT CACAATAAGG AATTGCAAAT ACTACTGAAA 
CCAGGTGGAG AGGACCAGGC CCTGGAGTAT TACACAAAAC ACACCTCACA GATAGAGATA 
GTTCGACAGG ATCGCACTAT GGAACAGATT GTGTTTCCTG TACCAAATAT CTGCTCTTTT 
CTGACAAATG AGTCCAAGCT GCGTGTGTAC TATGGTACAG AGAGAGACGA GCAAGGCAGT 
AAAATAAATG ACTTCTTCTT GCATGCAGAC GACCTCTTTA ATGAAATGAG GTGGCAGAAA 
AAACTAAGAG CTCAGCCAGT GCTGTACTGG TGCAGCAGGA ACATGTCGTT TTGGAGTAAC 
GTTTCCTTTA ATCTGGCGGT GCTCATTAAT TTGCTGGTGG CATTTTTCTA CCCTCTGGAT 
GGAGTGAGCG AAAGCCAGCT GGAACCTTCT CTGTCTCTGT TGCTGTGGGT TTGTTTGTTG 
GGCTCACTGG GCTTCGTGTT GATGTCCCCG AGGCCAAATG CAGTGCGGGT GCTGGTCATC 
TCCACTGTCC TGCAGTTGGG CTTCTCTGTA GGGCTGCAGC ACATGCTCAC ACTCCTGGGA 
GCTTTCAATG TTTGTAATAA GATCGTGTTC ATGCTGAGCT TTGTGGGTAA CCGTGGGTCA 
TTCACCCGGG GTTATCGGGT GATGGTCATG GATAGAGAGT TCCTGTTTCA TCTGCTGTAC 
CTTCTCATCT GCATACTGGG CCTTTTTGGA CACGTCTTCT TTTACAGTCT TCTGTTGTTT 
GACCTGGTGA ACCGGGAAGA GACTCTTCTG AACGTCATCA AGAGTGTGAC TCGAAACGGC 
CGCTCCATCG TCTTGACCGC AGTGCTGGGC CTCATCCTGG TCTACCTCTT TTCTATTGTT 
GGATATATGT TTTTCAAAGA TGACTTCATC CTGGAGGTGA ACCGCATCTC CAACGGCACT 
CTGGAGGAGG GGGTGACTTT ACCTGCTGGT TTTTGTGATG CGAGAAACGG GTCGTGTCTT 
TCTGATGATG TTGACGAGGA TGACGTGGAG CGCGCGTGTG ATTCTCTATG GATGTGTATG 
ATCACTGTAC TCAGTCATGG CCTCAGGAGC GGTGGAGGAG TTGGAGATGT CCTGCGCAAA 
CCGTCTAAAG AGGAGCGTCT GTTCGCCGCA CGGGTGGTGT ACGATCTCCT GTTCTTCTTC 
CTGGTGATCA TCATTGTGTT GAATCTGATC TTCGGTGTGA TCATTGACAC ATTTGCAGAT 
CTGAGAAGCG AGAAGCAGAG AAAAGAGGAG ATCTTGAAGA CCACATGCTT CATCTGTGGT 
CTGGAGAGAG ATAAGTTTGA TAATAAAACG GTGACGTTTG AAGAGCACAT CAAGGAGGAG 
CACAACCTGT GGCATTACCT CTTCTTCATA GTGCTGGTGC GGGTCAAAGA CTCCACCGAA 
TACACGGGAC CCGAGAGCTA CGTCGCTCAG ATGATCAAGG AGCACAATCT GGACTGGTTT 
CCTCGTATGA GGGCGATGTC TCTGGTCAGC AGTGATGGAG AAGGTGAACA GAATGAACTG 
CGCAGCTTAC AGGAGAAACT CGAGTCCACA ATGAGGCTCG TCTCTAATCT TACCAATCAG 
CTCACAGAGC TCAAAGAGCA GATGACTGAG CAAAGGAAAC ACAAGCAGAG GCTCGGTCTG 
CTGGGAAACC CCGCCCACCT CAACATCAAC CCACAGCAGC CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212153.1
CDS	148..8235
Translation	MVDKLSSFLHIGDVCSLYAEGSTSGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGTSTTDTVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDAAGNEGSWFYIQPFYKLRSIGDNVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCTTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDEHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEMDSEHEVIRARARNPQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSPLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAAKLEKGTITQNERRSVTKLLEDLVYFVVDIPSSAQDVLEITVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRYSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDPANADILIETKLVLSRFEVAGTVLGEGAEEEEEDEEEVWLFWKDSGGEVKSKSIRELAQDAKDGHTEDQEVINYYRYQLNLFARMCLDRQYLAINKISAQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSQISIDDYDNDGTSSDEVKERFSQTMEFVENYLRDVVCQSFPFSDKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHISTPFHIKLDREPGKGSNVMRSIHGVGELMTQVVLRGGGFLPASSHNPPDRDEVKAQSEPQKQDILVMDTKLKIIEILQFILNVRLDYRISCLLCIFKTEFDESNSQSEPSLSPESPASVQGALDFEHIEEQAEGIFGGSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALHLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSDSGLDAGEVTPEAHHKGVINSSRSNKPKVESTSSSNYRVVKEILLRLSKLCVLEGISGKKNKKQQQRLLRNMGAHSVVLELLQIPYEKGEDVQMQEIMTLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAVTMQHIFMNNFQLCSEINERVVQHFVHCIETHGRSVHYLKFLQTIVKAENKFIKKCQDIVMAELVTAGEDVLVFYNDRASFQSLVQMMRLERERLDESSALRYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKMAYVNFLNHCYVDTEVEMKEIYTSNHMWKLFDDFLVDVCRVCNNTSDRKHADTVLERYVTETIMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQGVFRVYHCNWLLPGQKGNVEACIKVLSDVAKGRAIAIPVDLDCQVNNLFMKSNNIVQKTALSWRLSVRNATRRDSVLTTSRDYRNIIERLQDIVSALEERLRPLVQAELSVLVDVLHRPELLFPEHTEAHRKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKLLGYDDEMDVTEVVDISLPPKQQEDRQRGEALRQLLVNRYYGFRSGGRRESLTSFGNSTLTPVGPIKSQPGGLSRAEMSLMEVQCHLDREGASDLVIDLIMNTTSDRVFHESILLAIALLEGGNTIIQHSFFKRLTEDKNSEKFFRVFYDRMKVAQVEIKATVTVNTSDLGNKRRDDNTPDKDTPQRRRGKDSGVVVTDDAREQLLEASAATKKAFGSYRRDADPEETFGTADGDKGGGDKGTEQGEMSPVILIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINQHNVALINQTVESLTEYCQGPCHDNQNCIATHESNGIDIIIALILNDINPLGRKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVMKMAYQQGEAEFEDEEQENGEDHAASPRNVGHNIYILAHQLSRHNKELQILLKPGGEDQALEYYTKHTSQIEIVRQDRTMEQIVFPVPNICSFLTNESKLRVYYGTERDEQGSKINDFFLHADDLFNEMRWQKKLRAQPVLYWCSRNMSFWSNVSFNLAVLINLLVAFFYPLDGVSESQLEPSLSLLLWVCLLGSLGFVLMSPRPNAVRVLVISTVLQLGFSVGLQHMLTLLGAFNVCNKIVFMLSFVGNRGSFTRGYRVMVMDREFLFHLLYLLICILGLFGHVFFYSLLLFDLVNREETLLNVIKSVTRNGRSIVLTAVLGLILVYLFSIVGYMFFKDDFILEVNRISNGTLEEGVTLPAGFCDARNGSCLSDDVDEDDVERACDSLWMCMITVLSHGLRSGGGVGDVLRKPSKEERLFAARVVYDLLFFFLVIIIVLNLIFGVIIDTFADLRSEKQRKEEILKTTCFICGLERDKFDNKTVTFEEHIKEEHNLWHYLFFIVLVRVKDSTEYTGPESYVAQMIKEHNLDWFPRMRAMSLVSSDGEGEQNELRSLQEKLESTMRLVSNLTNQLTELKEQMTEQRKHKQRLGLLGNPAHLNINPQQPA

Target ORF information:

RefSeq Version	XM_017356664.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356664.2

ORF Insert Sequence:

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GATGGATTCG 
GAGCATGAAG TGATTCGGGC TCGTGCACGT AACCCTCAGG ATAAGGTGAT GTACACACTG 
GTGTCTGTTC CTGATGGAAA TGACATATCC TCCATCTTTG AGCTTGACCC TACAACTTTG 
AGGGGCGGAG ACTCACTTGT GCCAAGAAAC TCGTATGTGA GGCTCAGGCA TCTCTGCACC 
AACACGTGGG TCCACAGCAC CAACCAGCCA ATAGATAAAG AGGAAGAGAA ACCGGTCATG 
CTCAGAATAG GAACGTCTCC TCTGAAGGAA GATAAAGAGG CGTTTGCTAT AGTACCAGTC 
TCTCCAGCTG AGGTTCGAGA TCTGGATTTT GCTAATGATG CTAGTAAAGT GTTGGCTTCC 
ATTGCTGCCA AACTGGAGAA GGGCACCATT ACCCAGAATG AGAGGAGGTC AGTGACGAAG 
CTGTTGGAGG ATCTGGTGTA TTTTGTGGTG GACATACCCA GCAGTGCTCA AGATGTGCTG 
GAGATCACAG TCAACAAACC CAACAGAGAG CGACAGAAAC TCATGAGAGA GCAGAACATT 
CTTAAACAGA TCTTCAAGCT GCTGCAGGCG CCATTTACAG ACAGCGGGGA CGGCCCTATG 
TTGCGATTAG AAGAGCTCGC AGACCAGCGT CATGCACCCT TTCGACACAT TTGCAGACTC 
TGTTACAGAG TCCTACGTTA CTCACAACAG GACTATCGCA AAAATCAGGA GTACATAGCA 
AAGCAGTTCC GCTTCATGCA GAAGCAGATA GGATATGATG TGCTGGCTGA AGACACAATT 
ACAGCCCTTC TGCACAACAA CAGGAAGCTC CTAGAGAAAC ACATCACAGC AGCTGAAATA 
GATACCTTTG TCAGTCTGGT CCGCAAGAAC AGAGAGCCCA GATTTCTGGA TTACTTGTCA 
GATCTGTGTG TGTCGATGAA TAAATCCATC CCGGTCACTC AAGAGCTCAT CTGTAATGCT 
GTGCTGGACC CAGCCAATGC AGACATTCTC ATCGAAACCA AGCTTGTGTT GTCACGGTTT 
GAGGTGGCAG GAACGGTGTT GGGTGAAGGT GCGGAGGAGG AAGAGGAGGA TGAAGAGGAG 
GTCTGGCTGT TCTGGAAGGA CAGTGGAGGA GAGGTGAAGA GTAAAAGCAT ACGAGAGCTT 
GCACAGGACG CAAAAGATGG ACATACAGAG GACCAGGAAG TCATCAATTA TTACAGATAT 
CAGCTGAATT TGTTTGCTCG GATGTGTCTG GATCGACAGT ATCTTGCGAT TAACAAGATA 
TCAGCACAGC TGGATGTGGA TCTGATTCTG CGCTGCATGT CAGATGAAGA TTTGCCCTTT 
GACCTCAGAG CATCGTTCTG CCGCATGATG CTGCACATGC ATGTAGACCG TGACCCTCAG 
GAGCAGGTCA CCCCGGTCAA ATATGCTCGA CTGTGGTCTG AAATCCCATC ACAAATTTCC 
ATTGATGATT ATGATAATGA TGGGACGTCA AGTGATGAAG TGAAAGAGCG CTTCTCTCAG 
ACGATGGAGT TTGTGGAGAA TTACCTGAGG GATGTTGTCT GCCAGAGTTT CCCGTTCTCT 
GATAAGGAGA AAAACAAACT CACTTTTGAG GTGGTGAACC TGGCAAGAAA CCTGATCTAT 
TTTGGTTTCT ATAACTTCAG TGATCTGCTA AGGTTAACAA AAATCCTATT GGCAATCCTG 
GACTGTGTCC ACATAAGCAC TCCCTTCCAC ATCAAGCTGG ATAGAGAACC CGGTAAAGGA 
AGTAACGTGA TGCGCTCCAT CCATGGGGTG GGAGAGCTGA TGACACAGGT GGTGTTGAGG 
GGTGGGGGAT TTCTGCCAGC CTCCAGCCAC AATCCTCCTG ACAGAGACGA AGTGAAAGCA 
CAAAGTGAAC CTCAGAAACA GGACATACTG GTGATGGACA CTAAACTCAA GATCATCGAG 
ATCCTGCAGT TTATTCTGAA TGTGCGGTTG GACTACAGGA TCTCCTGCCT CCTCTGTATC 
TTTAAGACAG AGTTTGATGA AAGTAACTCT CAAAGTGAAC CATCTTTAAG CCCAGAATCT 
CCTGCCAGCG TTCAAGGTGC GCTAGACTTT GAGCACATTG AAGAGCAGGC GGAGGGTATT 
TTTGGAGGAA GTGAAGAGAA CACCCCGCTG GATCTTGATG ATCATGGTGG ACGGACATTT 
CTGAGGGTCC TGCTTCACCT GACCATGCAT GACTATCCTC CGCTGGTATC TCGAGCTCTT 
CACCTGCTCT TCAGACACTT TAGCCAACGC CAGGAGGTCC TACAGGCCTT CAAACAGGTT 
CAGCTCCTGG TGACCAGTCA GGATGTGGAA AACTATAAAC AGATCAAGTC AGATTTGGAC 
CAGCTGAGGT CAATAGTAGA GAAATCAGAG TTGTGGGTGT ATAAGAGACA AGGCTCAGAC 
TCAGGACTGG ATGCTGGAGA AGTCACCCCT GAAGCCCACC ACAAGGGTGT CATCAACTCG 
AGTAGATCAA ACAAACCCAA AGTGGAAAGT ACCAGCAGCT CGAACTACAG AGTCGTGAAA 
GAGATCCTGC TGAGGCTGAG CAAACTGTGT GTTTTGGAGG GCATCTCTGG CAAGAAGAAT 
AAGAAACAGC AGCAGCGGCT CCTCAGGAAC ATGGGAGCTC ACAGTGTGGT CCTCGAACTG 
TTGCAAATCC CTTATGAGAA GGGGGAGGAT GTCCAGATGC AGGAGATCAT GACCTTAGCA 
CATCAGTTCC TTCAAAACTT CTGTGCTGGA AATCAGCAGA ACCAGGCACT TCTACACAAA 
CACATCAACC TGTTCCTCAA CCCTGGGATC CTGGAGGCGG TGACCATGCA GCATATCTTC 
ATGAATAATT TCCAGTTGTG TAGTGAGATT AATGAGCGGG TCGTGCAGCA TTTTGTGCAC 
TGCATCGAGA CACACGGCCG CAGCGTGCAC TACCTCAAGT TCCTGCAGAC CATTGTCAAG 
GCAGAGAACA AGTTCATCAA GAAATGCCAA GACATCGTCA TGGCTGAGCT GGTGACTGCA 
GGTGAGGATG TGCTGGTGTT CTATAATGAC CGGGCGTCTT TTCAGTCATT GGTTCAGATG 
ATGCGTCTTG AGCGGGAACG ACTTGATGAG AGCAGTGCAC TCAGGTATCA CATTCACCTG 
GTGGAGTTGC TGGCTGTGTG CACTGAAGGC AAGAACGTCT ACACTGAGAT CAAATGCAAC 
TCGCTGCTCC CTCTCGATGA TATTGTACGT GTGGTCACAC ATGAGGACTG CATCCCTGAG 
GTGAAGATGG CCTACGTGAA CTTCCTGAAT CACTGTTATG TTGATACAGA GGTTGAGATG 
AAGGAGATCT ACACCAGCAA TCACATGTGG AAACTTTTTG ATGATTTTCT GGTGGACGTC 
TGCAGAGTGT GCAATAACAC AAGTGACCGT AAACACGCTG ACACAGTTCT GGAGAGATAC 
GTTACAGAGA CCATAATGAG CATCGTCACC ACCTTTTTCA GCTCACCATT CTCAGACCAG 
AGCACAAGTT TGCAGACCCG GCAGCCGGTG TTTGTGCAGC TGCTGCAGGG AGTGTTTCGT 
GTATATCACT GCAACTGGCT TCTCCCAGGT CAGAAGGGGA ATGTGGAAGC CTGCATCAAA 
GTGCTGAGTG ACGTGGCCAA AGGCAGAGCC ATAGCCATTC CTGTGGATCT GGACTGTCAG 
GTGAATAATC TGTTCATGAA GTCCAACAAC ATTGTGCAGA AAACAGCCCT CAGCTGGAGA 
CTCTCTGTCC GTAACGCGAC TCGGAGAGAC TCTGTTCTGA CCACCTCTCG AGACTATCGC 
AACATCATTG AAAGACTACA GGATATAGTG TCTGCATTGG AGGAGCGGTT GAGGCCTCTG 
GTTCAGGCTG AGTTGTCAGT GTTGGTGGAT GTTCTCCATC GCCCAGAGCT GCTGTTTCCA 
GAGCACACTG AAGCCCACCG CAAGTGTGAG AGTGGAGGCT TCATCTGCAA GTTGATAAAA 
CACACTAAGC AGCTTCTAGA AGAAAACGAA GAGAGGCTTT GCATCAAAGT CCTTCAGACA 
CTCCGAGAGA TGATGACCAA AGACAGAGGA TATGGAGAGA AGCTTCTGGG ATATGATGAT 
GAGATGGATG TCACGGAGGT GGTGGATATC AGTCTGCCAC CCAAACAGCA GGAGGATCGG 
CAGAGGGGGG AGGCCCTTCG GCAGCTGCTC GTCAATCGTT ACTATGGCTT CAGGAGTGGT 
GGGCGGAGAG AAAGTCTGAC CTCGTTTGGT AACTCCACCC TAACTCCTGT CGGGCCAATC 
AAGAGCCAGC CAGGGGGGTT GAGCAGGGCT GAGATGTCAT TAATGGAGGT GCAGTGTCAT 
CTAGACCGTG AAGGGGCATC GGATCTTGTC ATTGATCTCA TCATGAACAC TACCAGCGAC 
CGCGTCTTCC ATGAAAGCAT TCTGTTGGCC ATCGCACTGT TGGAGGGCGG AAACACCATC 
ATACAGCATT CCTTCTTCAA ACGTTTAACA GAAGACAAAA ATTCAGAGAA GTTTTTCAGG 
GTGTTTTATG ACAGAATGAA AGTGGCTCAG GTGGAGATCA AAGCCACGGT TACTGTCAAC 
ACTAGTGACC TCGGCAACAA GCGGCGTGAC GACAACACTC CAGACAAAGA TACTCCACAA 
CGCCGCAGAG GTAAAGACTC CGGTGTGGTG GTGACAGATG ATGCTCGTGA GCAGCTGTTG 
GAGGCTTCAG CTGCGACTAA GAAGGCCTTT GGTTCATATC GTCGAGATGC TGATCCCGAG 
GAGACGTTTG GCACGGCTGA TGGAGATAAA GGAGGTGGAG ATAAAGGCAC AGAGCAGGGC 
GAGATGAGCC CTGTCATCCT CATAATGCAG CCAATCCTGC GCTTCCTGCA GCTGCTGTGC 
GAAAACCACA ATCGAGACCT GCAGAATTTT CTGCGCTGTC AGAACAATAA AAACAACTAT 
AATCTGGTGT GTGAGACGCT GCAGTTCCTG GACTGTATCT GTGGCAGCAC AACAGGAGGC 
CTGGGGCTGT TAGGACTCTA CATCAACCAG CATAACGTAG CGCTCATTAA TCAGACTGTA 
GAGAGCCTCA CTGAATACTG TCAAGGTCCC TGCCATGACA ATCAGAATTG CATTGCCACC 
CATGAGTCTA ATGGCATCGA CATCATTATT GCACTGATTC TGAATGATAT TAATCCACTG 
GGCAGGAAGA GAATGGATCT GGTTCTAGAA CTAAAGAATA ATGCATCTAA GCTGCTGCTG 
GCCATCATGG AGAGCCGTCA TGACAGTGAG AATGCAGAAA GAATTCTGTA CAACATGAGA 
CCAAAAGAAC TAGTGGAGGT GATGAAAATG GCCTACCAGC AGGGGGAGGC AGAGTTTGAG 
GATGAGGAAC AGGAGAATGG AGAGGATCAT GCAGCATCGC CTCGCAATGT TGGACACAAC 
ATCTATATTC TTGCACATCA GCTGTCGCGT CACAATAAGG AATTGCAAAT ACTACTGAAA 
CCAGGTGGAG AGGACCAGGC CCTGGAGTAT TACACAAAAC ACACCTCACA GATAGAGATA 
GTTCGACAGG ATCGCACTAT GGAACAGATT GTGTTTCCTG TACCAAATAT CTGCTCTTTT 
CTGACAAATG AGTCCAAGCT GCGTGTGTAC TATGGTACAG AGAGAGACGA GCAAGGCAGT 
AAAATAAATG ACTTCTTCTT GCATGCAGAC GACCTCTTTA ATGAAATGAG GTGGCAGAAA 
AAACTAAGAG CTCAGCCAGT GCTGTACTGG TGCAGCAGGA ACATGTCGTT TTGGAGTAAC 
GTTTCCTTTA ATCTGGCGGT GCTCATTAAT TTGCTGGTGG CATTTTTCTA CCCTCTGGAT 
GGAGTGAGCG AAAGCCAGCT GGAACCTTCT CTGTCTCTGT TGCTGTGGGT TTGTTTGTTG 
GGCTCACTGG GCTTCGTGTT GATGTCCCCG AGGCCAAATG CAGTGCGGGT GCTGGTCATC 
TCCACTGTCC TGCAGTTGGG CTTCTCTGTA GGGCTGCAGC ACATGCTCAC ACTCCTGGGA 
GCTTTCAATG TTTGTAATAA GATCGTGTTC ATGCTGAGCT TTGTGGGTAA CCGTGGGTCA 
TTCACCCGGG GTTATCGGGT GATGGTCATG GATAGAGAGT TCCTGTTTCA TCTGCTGTAC 
CTTCTCATCT GCATACTGGG CCTTTTTGGA CACGTCTTCT TTTACAGTCT TCTGTTGTTT 
GACCTGGTGA ACCGGGAAGA GACTCTTCTG AACGTCATCA AGAGTGTGAC TCGAAACGGC 
CGCTCCATCG TCTTGACCGC AGTGCTGGGC CTCATCCTGG TCTACCTCTT TTCTATTGTT 
GGATATATGT TTTTCAAAGA TGACTTCATC CTGGAGGTGA ACCGCATCTC CAACGGCACT 
CTGGAGGAGG GGGTGACTTT ACCTGCTGGT TTTTGTGATG CGAGAAACGG GTCGTGTCTT 
TCTGATGATG TTGACGAGGA TGACGTGGAG CGCGCGTGTG ATTCTCTATG GATGTGTATG 
ATCACTGTAC TCAGTCATGG CCTCAGGAGC GGTGGAGGAG TTGGAGATGT CCTGCGCAAA 
CCGTCTAAAG AGGAGCGTCT GTTCGCCGCA CGGGTGGTGT ACGATCTCCT GTTCTTCTTC 
CTGGTGATCA TCATTGTGTT GAATCTGATC TTCGGTGTGA TCATTGACAC ATTTGCAGAT 
CTGAGAAGCG AGAAGCAGAG AAAAGAGGAG ATCTTGAAGA CCACATGCTT CATCTGTGGT 
CTGGAGAGAG ATAAGTTTGA TAATAAAACG GTGACGTTTG AAGAGCACAT CAAGGAGGAG 
CACAACCTGT GGCATTACCT CTTCTTCATA GTGCTGGTGC GGGTCAAAGA CTCCACCGAA 
TACACGGGAC CCGAGAGCTA CGTCGCTCAG ATGATCAAGG AGCACAATCT GGACTGGTTT 
CCTCGTATGA GGGCGATGTC TCTGGTCAGC AGTGATGGAG AAGGTGAACA GAATGAACTG 
CGCAGCTTAC AGGAGAAACT CGAGTCCACA ATGAGGCTCG TCTCTAATCT TACCAATCAG 
CTCACAGAGC TCAAAGAGCA GATGACTGAG CAAAGGAAAC ACAAGCAGAG GCTCGGTCTG 
CTGGGAAACC CCGCCCACCT CAACATCAAC CCACAGCAGC CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itpr1a ( XM_017356664.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212153.1 Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X4, mRNA.

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CloneID	ODa59493
Clone ID Related Accession (Same CDS sequence)	XM_017356664.2 , XM_017356664.1
Accession Version	XM_017356664.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GATGGATTCG 
GAGCATGAAG TGATTCGGGC TCGTGCACGT AACCCTCAGG ATAAGGTGAT GTACACACTG 
GTGTCTGTTC CTGATGGAAA TGACATATCC TCCATCTTTG AGCTTGACCC TACAACTTTG 
AGGGGCGGAG ACTCACTTGT GCCAAGAAAC TCGTATGTGA GGCTCAGGCA TCTCTGCACC 
AACACGTGGG TCCACAGCAC CAACCAGCCA ATAGATAAAG AGGAAGAGAA ACCGGTCATG 
CTCAGAATAG GAACGTCTCC TCTGAAGGAA GATAAAGAGG CGTTTGCTAT AGTACCAGTC 
TCTCCAGCTG AGGTTCGAGA TCTGGATTTT GCTAATGATG CTAGTAAAGT GTTGGCTTCC 
ATTGCTGCCA AACTGGAGAA GGGCACCATT ACCCAGAATG AGAGGAGGTC AGTGACGAAG 
CTGTTGGAGG ATCTGGTGTA TTTTGTGGTG GACATACCCA GCAGTGCTCA AGATGTGCTG 
GAGATCACAG TCAACAAACC CAACAGAGAG CGACAGAAAC TCATGAGAGA GCAGAACATT 
CTTAAACAGA TCTTCAAGCT GCTGCAGGCG CCATTTACAG ACAGCGGGGA CGGCCCTATG 
TTGCGATTAG AAGAGCTCGC AGACCAGCGT CATGCACCCT TTCGACACAT TTGCAGACTC 
TGTTACAGAG TCCTACGTTA CTCACAACAG GACTATCGCA AAAATCAGGA GTACATAGCA 
AAGCAGTTCC GCTTCATGCA GAAGCAGATA GGATATGATG TGCTGGCTGA AGACACAATT 
ACAGCCCTTC TGCACAACAA CAGGAAGCTC CTAGAGAAAC ACATCACAGC AGCTGAAATA 
GATACCTTTG TCAGTCTGGT CCGCAAGAAC AGAGAGCCCA GATTTCTGGA TTACTTGTCA 
GATCTGTGTG TGTCGATGAA TAAATCCATC CCGGTCACTC AAGAGCTCAT CTGTAATGCT 
GTGCTGGACC CAGCCAATGC AGACATTCTC ATCGAAACCA AGCTTGTGTT GTCACGGTTT 
GAGGTGGCAG GAACGGTGTT GGGTGAAGGT GCGGAGGAGG AAGAGGAGGA TGAAGAGGAG 
GTCTGGCTGT TCTGGAAGGA CAGTGGAGGA GAGGTGAAGA GTAAAAGCAT ACGAGAGCTT 
GCACAGGACG CAAAAGATGG ACATACAGAG GACCAGGAAG TCATCAATTA TTACAGATAT 
CAGCTGAATT TGTTTGCTCG GATGTGTCTG GATCGACAGT ATCTTGCGAT TAACAAGATA 
TCAGCACAGC TGGATGTGGA TCTGATTCTG CGCTGCATGT CAGATGAAGA TTTGCCCTTT 
GACCTCAGAG CATCGTTCTG CCGCATGATG CTGCACATGC ATGTAGACCG TGACCCTCAG 
GAGCAGGTCA CCCCGGTCAA ATATGCTCGA CTGTGGTCTG AAATCCCATC ACAAATTTCC 
ATTGATGATT ATGATAATGA TGGGACGTCA AGTGATGAAG TGAAAGAGCG CTTCTCTCAG 
ACGATGGAGT TTGTGGAGAA TTACCTGAGG GATGTTGTCT GCCAGAGTTT CCCGTTCTCT 
GATAAGGAGA AAAACAAACT CACTTTTGAG GTGGTGAACC TGGCAAGAAA CCTGATCTAT 
TTTGGTTTCT ATAACTTCAG TGATCTGCTA AGGTTAACAA AAATCCTATT GGCAATCCTG 
GACTGTGTCC ACATAAGCAC TCCCTTCCAC ATCAAGCTGG ATAGAGAACC CGGTAAAGGA 
AGTAACGTGA TGCGCTCCAT CCATGGGGTG GGAGAGCTGA TGACACAGGT GGTGTTGAGG 
GGTGGGGGAT TTCTGCCAGC CTCCAGCCAC AATCCTCCTG ACAGAGACGA AGTGAAAGCA 
CAAAGTGAAC CTCAGAAACA GGACATACTG GTGATGGACA CTAAACTCAA GATCATCGAG 
ATCCTGCAGT TTATTCTGAA TGTGCGGTTG GACTACAGGA TCTCCTGCCT CCTCTGTATC 
TTTAAGACAG AGTTTGATGA AAGTAACTCT CAAAGTGAAC CATCTTTAAG CCCAGAATCT 
CCTGCCAGCG TTCAAGGTGC GCTAGACTTT GAGCACATTG AAGAGCAGGC GGAGGGTATT 
TTTGGAGGAA GTGAAGAGAA CACCCCGCTG GATCTTGATG ATCATGGTGG ACGGACATTT 
CTGAGGGTCC TGCTTCACCT GACCATGCAT GACTATCCTC CGCTGGTATC TCGAGCTCTT 
CACCTGCTCT TCAGACACTT TAGCCAACGC CAGGAGGTCC TACAGGCCTT CAAACAGGTT 
CAGCTCCTGG TGACCAGTCA GGATGTGGAA AACTATAAAC AGATCAAGTC AGATTTGGAC 
CAGCTGAGGT CAATAGTAGA GAAATCAGAG TTGTGGGTGT ATAAGAGACA AGGCTCAGAC 
TCAGGACTGG ATGCTGGAGA AGTCACCCCT GAAGCCCACC ACAAGGGTGT CATCAACTCG 
AGTAGATCAA ACAAACCCAA AGTGGAAAGT ACCAGCAGCT CGAACTACAG AGTCGTGAAA 
GAGATCCTGC TGAGGCTGAG CAAACTGTGT GTTTTGGAGG GCATCTCTGG CAAGAAGAAT 
AAGAAACAGC AGCAGCGGCT CCTCAGGAAC ATGGGAGCTC ACAGTGTGGT CCTCGAACTG 
TTGCAAATCC CTTATGAGAA GGGGGAGGAT GTCCAGATGC AGGAGATCAT GACCTTAGCA 
CATCAGTTCC TTCAAAACTT CTGTGCTGGA AATCAGCAGA ACCAGGCACT TCTACACAAA 
CACATCAACC TGTTCCTCAA CCCTGGGATC CTGGAGGCGG TGACCATGCA GCATATCTTC 
ATGAATAATT TCCAGTTGTG TAGTGAGATT AATGAGCGGG TCGTGCAGCA TTTTGTGCAC 
TGCATCGAGA CACACGGCCG CAGCGTGCAC TACCTCAAGT TCCTGCAGAC CATTGTCAAG 
GCAGAGAACA AGTTCATCAA GAAATGCCAA GACATCGTCA TGGCTGAGCT GGTGACTGCA 
GGTGAGGATG TGCTGGTGTT CTATAATGAC CGGGCGTCTT TTCAGTCATT GGTTCAGATG 
ATGCGTCTTG AGCGGGAACG ACTTGATGAG AGCAGTGCAC TCAGGTATCA CATTCACCTG 
GTGGAGTTGC TGGCTGTGTG CACTGAAGGC AAGAACGTCT ACACTGAGAT CAAATGCAAC 
TCGCTGCTCC CTCTCGATGA TATTGTACGT GTGGTCACAC ATGAGGACTG CATCCCTGAG 
GTGAAGATGG CCTACGTGAA CTTCCTGAAT CACTGTTATG TTGATACAGA GGTTGAGATG 
AAGGAGATCT ACACCAGCAA TCACATGTGG AAACTTTTTG ATGATTTTCT GGTGGACGTC 
TGCAGAGTGT GCAATAACAC AAGTGACCGT AAACACGCTG ACACAGTTCT GGAGAGATAC 
GTTACAGAGA CCATAATGAG CATCGTCACC ACCTTTTTCA GCTCACCATT CTCAGACCAG 
AGCACAAGTT TGCAGACCCG GCAGCCGGTG TTTGTGCAGC TGCTGCAGGG AGTGTTTCGT 
GTATATCACT GCAACTGGCT TCTCCCAGGT CAGAAGGGGA ATGTGGAAGC CTGCATCAAA 
GTGCTGAGTG ACGTGGCCAA AGGCAGAGCC ATAGCCATTC CTGTGGATCT GGACTGTCAG 
GTGAATAATC TGTTCATGAA GTCCAACAAC ATTGTGCAGA AAACAGCCCT CAGCTGGAGA 
CTCTCTGTCC GTAACGCGAC TCGGAGAGAC TCTGTTCTGA CCACCTCTCG AGACTATCGC 
AACATCATTG AAAGACTACA GGATATAGTG TCTGCATTGG AGGAGCGGTT GAGGCCTCTG 
GTTCAGGCTG AGTTGTCAGT GTTGGTGGAT GTTCTCCATC GCCCAGAGCT GCTGTTTCCA 
GAGCACACTG AAGCCCACCG CAAGTGTGAG AGTGGAGGCT TCATCTGCAA GTTGATAAAA 
CACACTAAGC AGCTTCTAGA AGAAAACGAA GAGAGGCTTT GCATCAAAGT CCTTCAGACA 
CTCCGAGAGA TGATGACCAA AGACAGAGGA TATGGAGAGA AGCTTCTGGG ATATGATGAT 
GAGATGGATG TCACGGAGGT GGTGGATATC AGTCTGCCAC CCAAACAGCA GGAGGATCGG 
CAGAGGGGGG AGGCCCTTCG GCAGCTGCTC GTCAATCGTT ACTATGGCTT CAGGAGTGGT 
GGGCGGAGAG AAAGTCTGAC CTCGTTTGGT AACTCCACCC TAACTCCTGT CGGGCCAATC 
AAGAGCCAGC CAGGGGGGTT GAGCAGGGCT GAGATGTCAT TAATGGAGGT GCAGTGTCAT 
CTAGACCGTG AAGGGGCATC GGATCTTGTC ATTGATCTCA TCATGAACAC TACCAGCGAC 
CGCGTCTTCC ATGAAAGCAT TCTGTTGGCC ATCGCACTGT TGGAGGGCGG AAACACCATC 
ATACAGCATT CCTTCTTCAA ACGTTTAACA GAAGACAAAA ATTCAGAGAA GTTTTTCAGG 
GTGTTTTATG ACAGAATGAA AGTGGCTCAG GTGGAGATCA AAGCCACGGT TACTGTCAAC 
ACTAGTGACC TCGGCAACAA GCGGCGTGAC GACAACACTC CAGACAAAGA TACTCCACAA 
CGCCGCAGAG GTAAAGACTC CGGTGTGGTG GTGACAGATG ATGCTCGTGA GCAGCTGTTG 
GAGGCTTCAG CTGCGACTAA GAAGGCCTTT GGTTCATATC GTCGAGATGC TGATCCCGAG 
GAGACGTTTG GCACGGCTGA TGGAGATAAA GGAGGTGGAG ATAAAGGCAC AGAGCAGGGC 
GAGATGAGCC CTGTCATCCT CATAATGCAG CCAATCCTGC GCTTCCTGCA GCTGCTGTGC 
GAAAACCACA ATCGAGACCT GCAGAATTTT CTGCGCTGTC AGAACAATAA AAACAACTAT 
AATCTGGTGT GTGAGACGCT GCAGTTCCTG GACTGTATCT GTGGCAGCAC AACAGGAGGC 
CTGGGGCTGT TAGGACTCTA CATCAACCAG CATAACGTAG CGCTCATTAA TCAGACTGTA 
GAGAGCCTCA CTGAATACTG TCAAGGTCCC TGCCATGACA ATCAGAATTG CATTGCCACC 
CATGAGTCTA ATGGCATCGA CATCATTATT GCACTGATTC TGAATGATAT TAATCCACTG 
GGCAGGAAGA GAATGGATCT GGTTCTAGAA CTAAAGAATA ATGCATCTAA GCTGCTGCTG 
GCCATCATGG AGAGCCGTCA TGACAGTGAG AATGCAGAAA GAATTCTGTA CAACATGAGA 
CCAAAAGAAC TAGTGGAGGT GATGAAAATG GCCTACCAGC AGGGGGAGGC AGAGTTTGAG 
GATGAGGAAC AGGAGAATGG AGAGGATCAT GCAGCATCGC CTCGCAATGT TGGACACAAC 
ATCTATATTC TTGCACATCA GCTGTCGCGT CACAATAAGG AATTGCAAAT ACTACTGAAA 
CCAGGTGGAG AGGACCAGGC CCTGGAGTAT TACACAAAAC ACACCTCACA GATAGAGATA 
GTTCGACAGG ATCGCACTAT GGAACAGATT GTGTTTCCTG TACCAAATAT CTGCTCTTTT 
CTGACAAATG AGTCCAAGCT GCGTGTGTAC TATGGTACAG AGAGAGACGA GCAAGGCAGT 
AAAATAAATG ACTTCTTCTT GCATGCAGAC GACCTCTTTA ATGAAATGAG GTGGCAGAAA 
AAACTAAGAG CTCAGCCAGT GCTGTACTGG TGCAGCAGGA ACATGTCGTT TTGGAGTAAC 
GTTTCCTTTA ATCTGGCGGT GCTCATTAAT TTGCTGGTGG CATTTTTCTA CCCTCTGGAT 
GGAGTGAGCG AAAGCCAGCT GGAACCTTCT CTGTCTCTGT TGCTGTGGGT TTGTTTGTTG 
GGCTCACTGG GCTTCGTGTT GATGTCCCCG AGGCCAAATG CAGTGCGGGT GCTGGTCATC 
TCCACTGTCC TGCAGTTGGG CTTCTCTGTA GGGCTGCAGC ACATGCTCAC ACTCCTGGGA 
GCTTTCAATG TTTGTAATAA GATCGTGTTC ATGCTGAGCT TTGTGGGTAA CCGTGGGTCA 
TTCACCCGGG GTTATCGGGT GATGGTCATG GATAGAGAGT TCCTGTTTCA TCTGCTGTAC 
CTTCTCATCT GCATACTGGG CCTTTTTGGA CACGTCTTCT TTTACAGTCT TCTGTTGTTT 
GACCTGGTGA ACCGGGAAGA GACTCTTCTG AACGTCATCA AGAGTGTGAC TCGAAACGGC 
CGCTCCATCG TCTTGACCGC AGTGCTGGGC CTCATCCTGG TCTACCTCTT TTCTATTGTT 
GGATATATGT TTTTCAAAGA TGACTTCATC CTGGAGGTGA ACCGCATCTC CAACGGCACT 
CTGGAGGAGG GGGTGACTTT ACCTGCTGGT TTTTGTGATG CGAGAAACGG GTCGTGTCTT 
TCTGATGATG TTGACGAGGA TGACGTGGAG CGCGCGTGTG ATTCTCTATG GATGTGTATG 
ATCACTGTAC TCAGTCATGG CCTCAGGAGC GGTGGAGGAG TTGGAGATGT CCTGCGCAAA 
CCGTCTAAAG AGGAGCGTCT GTTCGCCGCA CGGGTGGTGT ACGATCTCCT GTTCTTCTTC 
CTGGTGATCA TCATTGTGTT GAATCTGATC TTCGGTGTGA TCATTGACAC ATTTGCAGAT 
CTGAGAAGCG AGAAGCAGAG AAAAGAGGAG ATCTTGAAGA CCACATGCTT CATCTGTGGT 
CTGGAGAGAG ATAAGTTTGA TAATAAAACG GTGACGTTTG AAGAGCACAT CAAGGAGGAG 
CACAACCTGT GGCATTACCT CTTCTTCATA GTGCTGGTGC GGGTCAAAGA CTCCACCGAA 
TACACGGGAC CCGAGAGCTA CGTCGCTCAG ATGATCAAGG AGCACAATCT GGACTGGTTT 
CCTCGTATGA GGGCGATGTC TCTGGTCAGC AGTGATGGAG AAGGTGAACA GAATGAACTG 
CGCAGCTTAC AGGAGAAACT CGAGTCCACA ATGAGGCTCG TCTCTAATCT TACCAATCAG 
CTCACAGAGC TCAAAGAGCA GATGACTGAG CAAAGGAAAC ACAAGCAGAG GCTCGGTCTG 
CTGGGAAACC CCGCCCACCT CAACATCAAC CCACAGCAGC CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212153.1
CDS	148..8235
Translation	MVDKLSSFLHIGDVCSLYAEGSTSGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGTSTTDTVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDAAGNEGSWFYIQPFYKLRSIGDNVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCTTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDEHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEMDSEHEVIRARARNPQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSPLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAAKLEKGTITQNERRSVTKLLEDLVYFVVDIPSSAQDVLEITVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRYSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDPANADILIETKLVLSRFEVAGTVLGEGAEEEEEDEEEVWLFWKDSGGEVKSKSIRELAQDAKDGHTEDQEVINYYRYQLNLFARMCLDRQYLAINKISAQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSQISIDDYDNDGTSSDEVKERFSQTMEFVENYLRDVVCQSFPFSDKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHISTPFHIKLDREPGKGSNVMRSIHGVGELMTQVVLRGGGFLPASSHNPPDRDEVKAQSEPQKQDILVMDTKLKIIEILQFILNVRLDYRISCLLCIFKTEFDESNSQSEPSLSPESPASVQGALDFEHIEEQAEGIFGGSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALHLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSDSGLDAGEVTPEAHHKGVINSSRSNKPKVESTSSSNYRVVKEILLRLSKLCVLEGISGKKNKKQQQRLLRNMGAHSVVLELLQIPYEKGEDVQMQEIMTLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAVTMQHIFMNNFQLCSEINERVVQHFVHCIETHGRSVHYLKFLQTIVKAENKFIKKCQDIVMAELVTAGEDVLVFYNDRASFQSLVQMMRLERERLDESSALRYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKMAYVNFLNHCYVDTEVEMKEIYTSNHMWKLFDDFLVDVCRVCNNTSDRKHADTVLERYVTETIMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQGVFRVYHCNWLLPGQKGNVEACIKVLSDVAKGRAIAIPVDLDCQVNNLFMKSNNIVQKTALSWRLSVRNATRRDSVLTTSRDYRNIIERLQDIVSALEERLRPLVQAELSVLVDVLHRPELLFPEHTEAHRKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKLLGYDDEMDVTEVVDISLPPKQQEDRQRGEALRQLLVNRYYGFRSGGRRESLTSFGNSTLTPVGPIKSQPGGLSRAEMSLMEVQCHLDREGASDLVIDLIMNTTSDRVFHESILLAIALLEGGNTIIQHSFFKRLTEDKNSEKFFRVFYDRMKVAQVEIKATVTVNTSDLGNKRRDDNTPDKDTPQRRRGKDSGVVVTDDAREQLLEASAATKKAFGSYRRDADPEETFGTADGDKGGGDKGTEQGEMSPVILIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINQHNVALINQTVESLTEYCQGPCHDNQNCIATHESNGIDIIIALILNDINPLGRKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVMKMAYQQGEAEFEDEEQENGEDHAASPRNVGHNIYILAHQLSRHNKELQILLKPGGEDQALEYYTKHTSQIEIVRQDRTMEQIVFPVPNICSFLTNESKLRVYYGTERDEQGSKINDFFLHADDLFNEMRWQKKLRAQPVLYWCSRNMSFWSNVSFNLAVLINLLVAFFYPLDGVSESQLEPSLSLLLWVCLLGSLGFVLMSPRPNAVRVLVISTVLQLGFSVGLQHMLTLLGAFNVCNKIVFMLSFVGNRGSFTRGYRVMVMDREFLFHLLYLLICILGLFGHVFFYSLLLFDLVNREETLLNVIKSVTRNGRSIVLTAVLGLILVYLFSIVGYMFFKDDFILEVNRISNGTLEEGVTLPAGFCDARNGSCLSDDVDEDDVERACDSLWMCMITVLSHGLRSGGGVGDVLRKPSKEERLFAARVVYDLLFFFLVIIIVLNLIFGVIIDTFADLRSEKQRKEEILKTTCFICGLERDKFDNKTVTFEEHIKEEHNLWHYLFFIVLVRVKDSTEYTGPESYVAQMIKEHNLDWFPRMRAMSLVSSDGEGEQNELRSLQEKLESTMRLVSNLTNQLTELKEQMTEQRKHKQRLGLLGNPAHLNINPQQPA

Target ORF information:

RefSeq Version	XM_017356664.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356664.1

ORF Insert Sequence:

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GATGGATTCG 
GAGCATGAAG TGATTCGGGC TCGTGCACGT AACCCTCAGG ATAAGGTGAT GTACACACTG 
GTGTCTGTTC CTGATGGAAA TGACATATCC TCCATCTTTG AGCTTGACCC TACAACTTTG 
AGGGGCGGAG ACTCACTTGT GCCAAGAAAC TCGTATGTGA GGCTCAGGCA TCTCTGCACC 
AACACGTGGG TCCACAGCAC CAACCAGCCA ATAGATAAAG AGGAAGAGAA ACCGGTCATG 
CTCAGAATAG GAACGTCTCC TCTGAAGGAA GATAAAGAGG CGTTTGCTAT AGTACCAGTC 
TCTCCAGCTG AGGTTCGAGA TCTGGATTTT GCTAATGATG CTAGTAAAGT GTTGGCTTCC 
ATTGCTGCCA AACTGGAGAA GGGCACCATT ACCCAGAATG AGAGGAGGTC AGTGACGAAG 
CTGTTGGAGG ATCTGGTGTA TTTTGTGGTG GACATACCCA GCAGTGCTCA AGATGTGCTG 
GAGATCACAG TCAACAAACC CAACAGAGAG CGACAGAAAC TCATGAGAGA GCAGAACATT 
CTTAAACAGA TCTTCAAGCT GCTGCAGGCG CCATTTACAG ACAGCGGGGA CGGCCCTATG 
TTGCGATTAG AAGAGCTCGC AGACCAGCGT CATGCACCCT TTCGACACAT TTGCAGACTC 
TGTTACAGAG TCCTACGTTA CTCACAACAG GACTATCGCA AAAATCAGGA GTACATAGCA 
AAGCAGTTCC GCTTCATGCA GAAGCAGATA GGATATGATG TGCTGGCTGA AGACACAATT 
ACAGCCCTTC TGCACAACAA CAGGAAGCTC CTAGAGAAAC ACATCACAGC AGCTGAAATA 
GATACCTTTG TCAGTCTGGT CCGCAAGAAC AGAGAGCCCA GATTTCTGGA TTACTTGTCA 
GATCTGTGTG TGTCGATGAA TAAATCCATC CCGGTCACTC AAGAGCTCAT CTGTAATGCT 
GTGCTGGACC CAGCCAATGC AGACATTCTC ATCGAAACCA AGCTTGTGTT GTCACGGTTT 
GAGGTGGCAG GAACGGTGTT GGGTGAAGGT GCGGAGGAGG AAGAGGAGGA TGAAGAGGAG 
GTCTGGCTGT TCTGGAAGGA CAGTGGAGGA GAGGTGAAGA GTAAAAGCAT ACGAGAGCTT 
GCACAGGACG CAAAAGATGG ACATACAGAG GACCAGGAAG TCATCAATTA TTACAGATAT 
CAGCTGAATT TGTTTGCTCG GATGTGTCTG GATCGACAGT ATCTTGCGAT TAACAAGATA 
TCAGCACAGC TGGATGTGGA TCTGATTCTG CGCTGCATGT CAGATGAAGA TTTGCCCTTT 
GACCTCAGAG CATCGTTCTG CCGCATGATG CTGCACATGC ATGTAGACCG TGACCCTCAG 
GAGCAGGTCA CCCCGGTCAA ATATGCTCGA CTGTGGTCTG AAATCCCATC ACAAATTTCC 
ATTGATGATT ATGATAATGA TGGGACGTCA AGTGATGAAG TGAAAGAGCG CTTCTCTCAG 
ACGATGGAGT TTGTGGAGAA TTACCTGAGG GATGTTGTCT GCCAGAGTTT CCCGTTCTCT 
GATAAGGAGA AAAACAAACT CACTTTTGAG GTGGTGAACC TGGCAAGAAA CCTGATCTAT 
TTTGGTTTCT ATAACTTCAG TGATCTGCTA AGGTTAACAA AAATCCTATT GGCAATCCTG 
GACTGTGTCC ACATAAGCAC TCCCTTCCAC ATCAAGCTGG ATAGAGAACC CGGTAAAGGA 
AGTAACGTGA TGCGCTCCAT CCATGGGGTG GGAGAGCTGA TGACACAGGT GGTGTTGAGG 
GGTGGGGGAT TTCTGCCAGC CTCCAGCCAC AATCCTCCTG ACAGAGACGA AGTGAAAGCA 
CAAAGTGAAC CTCAGAAACA GGACATACTG GTGATGGACA CTAAACTCAA GATCATCGAG 
ATCCTGCAGT TTATTCTGAA TGTGCGGTTG GACTACAGGA TCTCCTGCCT CCTCTGTATC 
TTTAAGACAG AGTTTGATGA AAGTAACTCT CAAAGTGAAC CATCTTTAAG CCCAGAATCT 
CCTGCCAGCG TTCAAGGTGC GCTAGACTTT GAGCACATTG AAGAGCAGGC GGAGGGTATT 
TTTGGAGGAA GTGAAGAGAA CACCCCGCTG GATCTTGATG ATCATGGTGG ACGGACATTT 
CTGAGGGTCC TGCTTCACCT GACCATGCAT GACTATCCTC CGCTGGTATC TCGAGCTCTT 
CACCTGCTCT TCAGACACTT TAGCCAACGC CAGGAGGTCC TACAGGCCTT CAAACAGGTT 
CAGCTCCTGG TGACCAGTCA GGATGTGGAA AACTATAAAC AGATCAAGTC AGATTTGGAC 
CAGCTGAGGT CAATAGTAGA GAAATCAGAG TTGTGGGTGT ATAAGAGACA AGGCTCAGAC 
TCAGGACTGG ATGCTGGAGA AGTCACCCCT GAAGCCCACC ACAAGGGTGT CATCAACTCG 
AGTAGATCAA ACAAACCCAA AGTGGAAAGT ACCAGCAGCT CGAACTACAG AGTCGTGAAA 
GAGATCCTGC TGAGGCTGAG CAAACTGTGT GTTTTGGAGG GCATCTCTGG CAAGAAGAAT 
AAGAAACAGC AGCAGCGGCT CCTCAGGAAC ATGGGAGCTC ACAGTGTGGT CCTCGAACTG 
TTGCAAATCC CTTATGAGAA GGGGGAGGAT GTCCAGATGC AGGAGATCAT GACCTTAGCA 
CATCAGTTCC TTCAAAACTT CTGTGCTGGA AATCAGCAGA ACCAGGCACT TCTACACAAA 
CACATCAACC TGTTCCTCAA CCCTGGGATC CTGGAGGCGG TGACCATGCA GCATATCTTC 
ATGAATAATT TCCAGTTGTG TAGTGAGATT AATGAGCGGG TCGTGCAGCA TTTTGTGCAC 
TGCATCGAGA CACACGGCCG CAGCGTGCAC TACCTCAAGT TCCTGCAGAC CATTGTCAAG 
GCAGAGAACA AGTTCATCAA GAAATGCCAA GACATCGTCA TGGCTGAGCT GGTGACTGCA 
GGTGAGGATG TGCTGGTGTT CTATAATGAC CGGGCGTCTT TTCAGTCATT GGTTCAGATG 
ATGCGTCTTG AGCGGGAACG ACTTGATGAG AGCAGTGCAC TCAGGTATCA CATTCACCTG 
GTGGAGTTGC TGGCTGTGTG CACTGAAGGC AAGAACGTCT ACACTGAGAT CAAATGCAAC 
TCGCTGCTCC CTCTCGATGA TATTGTACGT GTGGTCACAC ATGAGGACTG CATCCCTGAG 
GTGAAGATGG CCTACGTGAA CTTCCTGAAT CACTGTTATG TTGATACAGA GGTTGAGATG 
AAGGAGATCT ACACCAGCAA TCACATGTGG AAACTTTTTG ATGATTTTCT GGTGGACGTC 
TGCAGAGTGT GCAATAACAC AAGTGACCGT AAACACGCTG ACACAGTTCT GGAGAGATAC 
GTTACAGAGA CCATAATGAG CATCGTCACC ACCTTTTTCA GCTCACCATT CTCAGACCAG 
AGCACAAGTT TGCAGACCCG GCAGCCGGTG TTTGTGCAGC TGCTGCAGGG AGTGTTTCGT 
GTATATCACT GCAACTGGCT TCTCCCAGGT CAGAAGGGGA ATGTGGAAGC CTGCATCAAA 
GTGCTGAGTG ACGTGGCCAA AGGCAGAGCC ATAGCCATTC CTGTGGATCT GGACTGTCAG 
GTGAATAATC TGTTCATGAA GTCCAACAAC ATTGTGCAGA AAACAGCCCT CAGCTGGAGA 
CTCTCTGTCC GTAACGCGAC TCGGAGAGAC TCTGTTCTGA CCACCTCTCG AGACTATCGC 
AACATCATTG AAAGACTACA GGATATAGTG TCTGCATTGG AGGAGCGGTT GAGGCCTCTG 
GTTCAGGCTG AGTTGTCAGT GTTGGTGGAT GTTCTCCATC GCCCAGAGCT GCTGTTTCCA 
GAGCACACTG AAGCCCACCG CAAGTGTGAG AGTGGAGGCT TCATCTGCAA GTTGATAAAA 
CACACTAAGC AGCTTCTAGA AGAAAACGAA GAGAGGCTTT GCATCAAAGT CCTTCAGACA 
CTCCGAGAGA TGATGACCAA AGACAGAGGA TATGGAGAGA AGCTTCTGGG ATATGATGAT 
GAGATGGATG TCACGGAGGT GGTGGATATC AGTCTGCCAC CCAAACAGCA GGAGGATCGG 
CAGAGGGGGG AGGCCCTTCG GCAGCTGCTC GTCAATCGTT ACTATGGCTT CAGGAGTGGT 
GGGCGGAGAG AAAGTCTGAC CTCGTTTGGT AACTCCACCC TAACTCCTGT CGGGCCAATC 
AAGAGCCAGC CAGGGGGGTT GAGCAGGGCT GAGATGTCAT TAATGGAGGT GCAGTGTCAT 
CTAGACCGTG AAGGGGCATC GGATCTTGTC ATTGATCTCA TCATGAACAC TACCAGCGAC 
CGCGTCTTCC ATGAAAGCAT TCTGTTGGCC ATCGCACTGT TGGAGGGCGG AAACACCATC 
ATACAGCATT CCTTCTTCAA ACGTTTAACA GAAGACAAAA ATTCAGAGAA GTTTTTCAGG 
GTGTTTTATG ACAGAATGAA AGTGGCTCAG GTGGAGATCA AAGCCACGGT TACTGTCAAC 
ACTAGTGACC TCGGCAACAA GCGGCGTGAC GACAACACTC CAGACAAAGA TACTCCACAA 
CGCCGCAGAG GTAAAGACTC CGGTGTGGTG GTGACAGATG ATGCTCGTGA GCAGCTGTTG 
GAGGCTTCAG CTGCGACTAA GAAGGCCTTT GGTTCATATC GTCGAGATGC TGATCCCGAG 
GAGACGTTTG GCACGGCTGA TGGAGATAAA GGAGGTGGAG ATAAAGGCAC AGAGCAGGGC 
GAGATGAGCC CTGTCATCCT CATAATGCAG CCAATCCTGC GCTTCCTGCA GCTGCTGTGC 
GAAAACCACA ATCGAGACCT GCAGAATTTT CTGCGCTGTC AGAACAATAA AAACAACTAT 
AATCTGGTGT GTGAGACGCT GCAGTTCCTG GACTGTATCT GTGGCAGCAC AACAGGAGGC 
CTGGGGCTGT TAGGACTCTA CATCAACCAG CATAACGTAG CGCTCATTAA TCAGACTGTA 
GAGAGCCTCA CTGAATACTG TCAAGGTCCC TGCCATGACA ATCAGAATTG CATTGCCACC 
CATGAGTCTA ATGGCATCGA CATCATTATT GCACTGATTC TGAATGATAT TAATCCACTG 
GGCAGGAAGA GAATGGATCT GGTTCTAGAA CTAAAGAATA ATGCATCTAA GCTGCTGCTG 
GCCATCATGG AGAGCCGTCA TGACAGTGAG AATGCAGAAA GAATTCTGTA CAACATGAGA 
CCAAAAGAAC TAGTGGAGGT GATGAAAATG GCCTACCAGC AGGGGGAGGC AGAGTTTGAG 
GATGAGGAAC AGGAGAATGG AGAGGATCAT GCAGCATCGC CTCGCAATGT TGGACACAAC 
ATCTATATTC TTGCACATCA GCTGTCGCGT CACAATAAGG AATTGCAAAT ACTACTGAAA 
CCAGGTGGAG AGGACCAGGC CCTGGAGTAT TACACAAAAC ACACCTCACA GATAGAGATA 
GTTCGACAGG ATCGCACTAT GGAACAGATT GTGTTTCCTG TACCAAATAT CTGCTCTTTT 
CTGACAAATG AGTCCAAGCT GCGTGTGTAC TATGGTACAG AGAGAGACGA GCAAGGCAGT 
AAAATAAATG ACTTCTTCTT GCATGCAGAC GACCTCTTTA ATGAAATGAG GTGGCAGAAA 
AAACTAAGAG CTCAGCCAGT GCTGTACTGG TGCAGCAGGA ACATGTCGTT TTGGAGTAAC 
GTTTCCTTTA ATCTGGCGGT GCTCATTAAT TTGCTGGTGG CATTTTTCTA CCCTCTGGAT 
GGAGTGAGCG AAAGCCAGCT GGAACCTTCT CTGTCTCTGT TGCTGTGGGT TTGTTTGTTG 
GGCTCACTGG GCTTCGTGTT GATGTCCCCG AGGCCAAATG CAGTGCGGGT GCTGGTCATC 
TCCACTGTCC TGCAGTTGGG CTTCTCTGTA GGGCTGCAGC ACATGCTCAC ACTCCTGGGA 
GCTTTCAATG TTTGTAATAA GATCGTGTTC ATGCTGAGCT TTGTGGGTAA CCGTGGGTCA 
TTCACCCGGG GTTATCGGGT GATGGTCATG GATAGAGAGT TCCTGTTTCA TCTGCTGTAC 
CTTCTCATCT GCATACTGGG CCTTTTTGGA CACGTCTTCT TTTACAGTCT TCTGTTGTTT 
GACCTGGTGA ACCGGGAAGA GACTCTTCTG AACGTCATCA AGAGTGTGAC TCGAAACGGC 
CGCTCCATCG TCTTGACCGC AGTGCTGGGC CTCATCCTGG TCTACCTCTT TTCTATTGTT 
GGATATATGT TTTTCAAAGA TGACTTCATC CTGGAGGTGA ACCGCATCTC CAACGGCACT 
CTGGAGGAGG GGGTGACTTT ACCTGCTGGT TTTTGTGATG CGAGAAACGG GTCGTGTCTT 
TCTGATGATG TTGACGAGGA TGACGTGGAG CGCGCGTGTG ATTCTCTATG GATGTGTATG 
ATCACTGTAC TCAGTCATGG CCTCAGGAGC GGTGGAGGAG TTGGAGATGT CCTGCGCAAA 
CCGTCTAAAG AGGAGCGTCT GTTCGCCGCA CGGGTGGTGT ACGATCTCCT GTTCTTCTTC 
CTGGTGATCA TCATTGTGTT GAATCTGATC TTCGGTGTGA TCATTGACAC ATTTGCAGAT 
CTGAGAAGCG AGAAGCAGAG AAAAGAGGAG ATCTTGAAGA CCACATGCTT CATCTGTGGT 
CTGGAGAGAG ATAAGTTTGA TAATAAAACG GTGACGTTTG AAGAGCACAT CAAGGAGGAG 
CACAACCTGT GGCATTACCT CTTCTTCATA GTGCTGGTGC GGGTCAAAGA CTCCACCGAA 
TACACGGGAC CCGAGAGCTA CGTCGCTCAG ATGATCAAGG AGCACAATCT GGACTGGTTT 
CCTCGTATGA GGGCGATGTC TCTGGTCAGC AGTGATGGAG AAGGTGAACA GAATGAACTG 
CGCAGCTTAC AGGAGAAACT CGAGTCCACA ATGAGGCTCG TCTCTAATCT TACCAATCAG 
CTCACAGAGC TCAAAGAGCA GATGACTGAG CAAAGGAAAC ACAAGCAGAG GCTCGGTCTG 
CTGGGAAACC CCGCCCACCT CAACATCAAC CCACAGCAGC CAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itpr1a ( XM_017356665.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212154.1 Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X6, mRNA.

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CloneID	ODa59494
Clone ID Related Accession (Same CDS sequence)	XM_017356665.2 , XM_017356665.1
Accession Version	XM_017356665.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356665.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GGTAAATCCA 
GACTATGAAG AAGAATGCCT GGAGTCCCGC TCCTCTATGG ATTCGGAGCA TGAAGTGATT 
CGGGCTCGTG CACGTAACCC TCAGGATAAG GTGATGTACA CACTGGTGTC TGTTCCTGAT 
GGAAATGACA TATCCTCCAT CTTTGAGCTT GACCCTACAA CTTTGAGGGG CGGAGACTCA 
CTTGTGCCAA GAAACTCGTA TGTGAGGCTC AGGCATCTCT GCACCAACAC GTGGGTCCAC 
AGCACCAACC AGCCAATAGA TAAAGAGGAA GAGAAACCGG TCATGCTCAG AATAGGAACG 
TCTCCTCTGA AGGAAGATAA AGAGGCGTTT GCTATAGTAC CAGTCTCTCC AGCTGAGGTT 
CGAGATCTGG ATTTTGCTAA TGATGCTAGT AAAGTGTTGG CTTCCATTGC TGCCAAACTG 
GAGAAGGGCA CCATTACCCA GAATGAGAGG AGGTCAGTGA CGAAGCTGTT GGAGGATCTG 
GTGTATTTTG TGGTGGACAT ACCCAGCAGT GCTCAAGATG TGCTGGAGAT CACAGTCAAC 
AAACCCAACA GAGAGCGACA GAAACTCATG AGAGAGCAGA ACATTCTTAA ACAGATCTTC 
AAGCTGCTGC AGGCGCCATT TACAGACAGC GGGGACGGCC CTATGTTGCG ATTAGAAGAG 
CTCGCAGACC AGCGTCATGC ACCCTTTCGA CACATTTGCA GACTCTGTTA CAGAGTCCTA 
CGTTACTCAC AACAGGACTA TCGCAAAAAT CAGGAGTACA TAGCAAAGCA GTTCCGCTTC 
ATGCAGAAGC AGATAGGATA TGATGTGCTG GCTGAAGACA CAATTACAGC CCTTCTGCAC 
AACAACAGGA AGCTCCTAGA GAAACACATC ACAGCAGCTG AAATAGATAC CTTTGTCAGT 
CTGGTCCGCA AGAACAGAGA GCCCAGATTT CTGGATTACT TGTCAGATCT GTGTGTGTCG 
ATGAATAAAT CCATCCCGGT CACTCAAGAG CTCATCTGTA ATGCTGTGCT GGACCCAGCC 
AATGCAGACA TTCTCATCGA AACCAAGCTT GTGTTGTCAC GGTTTGAGGT GGCAGGAACG 
GTGTTGGGTG AAGGTGCGGA GGAGGAAGAG GAGGATGAAG AGGAGGTCTG GCTGTTCTGG 
AAGGACAGTG GAGGAGAGGT GAAGAGTAAA AGCATACGAG AGCTTGCACA GGACGCAAAA 
GATGGACATA CAGAGGACCA GGAAGTCATC AATTATTACA GATATCAGCT GAATTTGTTT 
GCTCGGATGT GTCTGGATCG ACAGTATCTT GCGATTAACA AGATATCAGC ACAGCTGGAT 
GTGGATCTGA TTCTGCGCTG CATGTCAGAT GAAGATTTGC CCTTTGACCT CAGAGCATCG 
TTCTGCCGCA TGATGCTGCA CATGCATGTA GACCGTGACC CTCAGGAGCA GGTCACCCCG 
GTCAAATATG CTCGACTGTG GTCTGAAATC CCATCACAAA TTTCCATTGA TGATTATGAT 
AATGATGGGA CGTCAAGTGA TGAAGTGAAA GAGCGCTTCT CTCAGACGAT GGAGTTTGTG 
GAGAATTACC TGAGGGATGT TGTCTGCCAG AGTTTCCCGT TCTCTGATAA GGAGAAAAAC 
AAACTCACTT TTGAGGTGGT GAACCTGGCA AGAAACCTGA TCTATTTTGG TTTCTATAAC 
TTCAGTGATC TGCTAAGGTT AACAAAAATC CTATTGGCAA TCCTGGACTG TGTCCACATA 
AGCACTCCCT TCCACATCAA GCTGGATAGA GAACCCGGTA AAGGAAGTAA CGTGATGCGC 
TCCATCCATG GGGTGGGAGA GCTGATGACA CAGGTGGTGT TGAGGGGTGG GGGATTTCTG 
CCAGCCTCCA GCCACAATCC TCCTGACAGA GACGAAGTGA AAGCACAAAG TGAACCTCAG 
AAACAGGACA TACTGGTGAT GGACACTAAA CTCAAGATCA TCGAGATCCT GCAGTTTATT 
CTGAATGTGC GGTTGGACTA CAGGATCTCC TGCCTCCTCT GTATCTTTAA GACAGAGTTT 
GATGAAAGTA ACTCTCAAAG TGAACCATCT TTAAGCCCAG AATCTCCTGC CAGCGTTCAA 
GGTGCGCTAG ACTTTGAGCA CATTGAAGAG CAGGCGGAGG GTATTTTTGG AGGAAGTGAA 
GAGAACACCC CGCTGGATCT TGATGATCAT GGTGGACGGA CATTTCTGAG GGTCCTGCTT 
CACCTGACCA TGCATGACTA TCCTCCGCTG GTATCTCGAG CTCTTCACCT GCTCTTCAGA 
CACTTTAGCC AACGCCAGGA GGTCCTACAG GCCTTCAAAC AGGTTCAGCT CCTGGTGACC 
AGTCAGGATG TGGAAAACTA TAAACAGATC AAGTCAGATT TGGACCAGCT GAGGTCAATA 
GTAGAGAAAT CAGAGTTGTG GGTGTATAAG AGACAAGGCT CAGACTCAGG ACTGGATGCT 
GGAGAAGTCA CCCCTGAAGC CCACCACAAG GGTGTCATCA ACTCGAGTAG ATCAAACAAA 
CCCAAAGTGG AAAGTACCAG CAGCTCGAAC TACAGAGTCG TGAAAGAGAT CCTGCTGAGG 
CTGAGCAAAC TGTGTGTTTT GGAGGGCATC TCTGGCAAGA AGAATAAGAA ACAGCAGCAG 
CGGCTCCTCA GGAACATGGG AGCTCACAGT GTGGTCCTCG AACTGTTGCA AATCCCTTAT 
GAGAAGGGGG AGGATGTCCA GATGCAGGAG ATCATGACCT TAGCACATCA GTTCCTTCAA 
AACTTCTGTG CTGGAAATCA GCAGAACCAG GCACTTCTAC ACAAACACAT CAACCTGTTC 
CTCAACCCTG GGATCCTGGA GGCGGTGACC ATGCAGCATA TCTTCATGAA TAATTTCCAG 
TTGTGTAGTG AGATTAATGA GCGGGTCGTG CAGCATTTTG TGCACTGCAT CGAGACACAC 
GGCCGCAGCG TGCACTACCT CAAGTTCCTG CAGACCATTG TCAAGGCAGA GAACAAGTTC 
ATCAAGAAAT GCCAAGACAT CGTCATGGCT GAGCTGGTGA CTGCAGGTGA GGATGTGCTG 
GTGTTCTATA ATGACCGGGC GTCTTTTCAG TCATTGGTTC AGATGATGCG TCTTGAGCGG 
GAACGACTTG ATGAGAGCAG TGCACTCAGG TATCACATTC ACCTGGTGGA GTTGCTGGCT 
GTGTGCACTG AAGGCAAGAA CGTCTACACT GAGATCAAAT GCAACTCGCT GCTCCCTCTC 
GATGATATTG TACGTGTGGT CACACATGAG GACTGCATCC CTGAGGTGAA GATGGCCTAC 
GTGAACTTCC TGAATCACTG TTATGTTGAT ACAGAGGTTG AGATGAAGGA GATCTACACC 
AGCAATCACA TGTGGAAACT TTTTGATGAT TTTCTGGTGG ACGTCTGCAG AGTGTGCAAT 
AACACAAGTG ACCGTAAACA CGCTGACACA GTTCTGGAGA GATACGTTAC AGAGACCATA 
ATGAGCATCG TCACCACCTT TTTCAGCTCA CCATTCTCAG ACCAGAGCAC AAGTTTGCAG 
ACCCGGCAGC CGGTGTTTGT GCAGCTGCTG CAGGGAGTGT TTCGTGTATA TCACTGCAAC 
TGGCTTCTCC CAGGTCAGAA GGGGAATGTG GAAGCCTGCA TCAAAGTGCT GAGTGACGTG 
GCCAAAGGCA GAGCCATAGC CATTCCTGTG GATCTGGACT GTCAGGTGAA TAATCTGTTC 
ATGAAGTCCA ACAACATTGT GCAGAAAACA GCCCTCAGCT GGAGACTCTC TGTCCGTAAC 
GCGACTCGGA GAGACTCTGT TCTGACCACC TCTCGAGACT ATCGCAACAT CATTGAAAGA 
CTACAGGATA TAGTGTCTGC ATTGGAGGAG CGGTTGAGGC CTCTGGTTCA GGCTGAGTTG 
TCAGTGTTGG TGGATGTTCT CCATCGCCCA GAGCTGCTGT TTCCAGAGCA CACTGAAGCC 
CACCGCAAGT GTGAGAGTGG AGGCTTCATC TGCAAGTTGA TAAAACACAC TAAGCAGCTT 
CTAGAAGAAA ACGAAGAGAG GCTTTGCATC AAAGTCCTTC AGACACTCCG AGAGATGATG 
ACCAAAGACA GAGGATATGG AGAGAAGGGG GAGGCCCTTC GGCAGCTGCT CGTCAATCGT 
TACTATGGCT TCAGGAGTGG TGGGCGGAGA GAAAGTCTGA CCTCGTTTGG TAACTCCACC 
CTAACTCCTG TCGGGCCAAT CAAGAGCCAG CCAGGGGGGT TGAGCAGGGC TGAGATGTCA 
TTAATGGAGG TGCAGTGTCA TCTAGACCGT GAAGGGGCAT CGGATCTTGT CATTGATCTC 
ATCATGAACA CTACCAGCGA CCGCGTCTTC CATGAAAGCA TTCTGTTGGC CATCGCACTG 
TTGGAGGGCG GAAACACCAT CATACAGCAT TCCTTCTTCA AACGTTTAAC AGAAGACAAA 
AATTCAGAGA AGTTTTTCAG GGTGTTTTAT GACAGAATGA AAGTGGCTCA GGTGGAGATC 
AAAGCCACGG TTACTGTCAA CACTAGTGAC CTCGGCAACA AGCGGCGTGA CGACAACACT 
CCAGACAAAG ATACTCCACA ACGCCGCAGA GGTAAAGACT CCGGTGTGGT GGTGACAGAT 
GATGCTCGTG AGCAGCTGTT GGAGGCTTCA GCTGCGACTA AGAAGGCCTT TGGTTCATAT 
CGTCGAGATG CTGATCCCGA GGAGACGTTT GGCACGGCTG ATGGAGATAA AGGAGGTGGA 
GATAAAGGCA CAGAGCAGGG CGAGATGAGC CCTGTCATCC TCATAATGCA GCCAATCCTG 
CGCTTCCTGC AGCTGCTGTG CGAAAACCAC AATCGAGACC TGCAGAATTT TCTGCGCTGT 
CAGAACAATA AAAACAACTA TAATCTGGTG TGTGAGACGC TGCAGTTCCT GGACTGTATC 
TGTGGCAGCA CAACAGGAGG CCTGGGGCTG TTAGGACTCT ACATCAACCA GCATAACGTA 
GCGCTCATTA ATCAGACTGT AGAGAGCCTC ACTGAATACT GTCAAGGTCC CTGCCATGAC 
AATCAGAATT GCATTGCCAC CCATGAGTCT AATGGCATCG ACATCATTAT TGCACTGATT 
CTGAATGATA TTAATCCACT GGGCAGGAAG AGAATGGATC TGGTTCTAGA ACTAAAGAAT 
AATGCATCTA AGCTGCTGCT GGCCATCATG GAGAGCCGTC ATGACAGTGA GAATGCAGAA 
AGAATTCTGT ACAACATGAG ACCAAAAGAA CTAGTGGAGG TGATGAAAAT GGCCTACCAG 
CAGGGGGAGG CAGAGTTTGA GGATGAGGAA CAGGAGAATG GAGAGGATCA TGCAGCATCG 
CCTCGCAATG TTGGACACAA CATCTATATT CTTGCACATC AGCTGTCGCG TCACAATAAG 
GAATTGCAAA TACTACTGAA ACCAGGTGGA GAGGACCAGG CCCTGGAGTA TTACACAAAA 
CACACCTCAC AGATAGAGAT AGTTCGACAG GATCGCACTA TGGAACAGAT TGTGTTTCCT 
GTACCAAATA TCTGCTCTTT TCTGACAAAT GAGTCCAAGC TGCGTGTGTA CTATGGTACA 
GAGAGAGACG AGCAAGGCAG TAAAATAAAT GACTTCTTCT TGCATGCAGA CGACCTCTTT 
AATGAAATGA GGTGGCAGAA AAAACTAAGA GCTCAGCCAG TGCTGTACTG GTGCAGCAGG 
AACATGTCGT TTTGGAGTAA CGTTTCCTTT AATCTGGCGG TGCTCATTAA TTTGCTGGTG 
GCATTTTTCT ACCCTCTGGA TGGAGTGAGC GAAAGCCAGC TGGAACCTTC TCTGTCTCTG 
TTGCTGTGGG TTTGTTTGTT GGGCTCACTG GGCTTCGTGT TGATGTCCCC GAGGCCAAAT 
GCAGTGCGGG TGCTGGTCAT CTCCACTGTC CTGCAGTTGG GCTTCTCTGT AGGGCTGCAG 
CACATGCTCA CACTCCTGGG AGCTTTCAAT GTTTGTAATA AGATCGTGTT CATGCTGAGC 
TTTGTGGGTA ACCGTGGGTC ATTCACCCGG GGTTATCGGG TGATGGTCAT GGATAGAGAG 
TTCCTGTTTC ATCTGCTGTA CCTTCTCATC TGCATACTGG GCCTTTTTGG ACACGTCTTC 
TTTTACAGTC TTCTGTTGTT TGACCTGGTG AACCGGGAAG AGACTCTTCT GAACGTCATC 
AAGAGTGTGA CTCGAAACGG CCGCTCCATC GTCTTGACCG CAGTGCTGGG CCTCATCCTG 
GTCTACCTCT TTTCTATTGT TGGATATATG TTTTTCAAAG ATGACTTCAT CCTGGAGGTG 
AACCGCATCT CCAACGGCAC TCTGGAGGAG GGGGTGACTT TACCTGCTGG TTTTTGTGAT 
GCGAGAAACG GGTCGTGTCT TTCTGATGAT GTTGACGAGG ATGACGTGGA GCGCGCGTGT 
GATTCTCTAT GGATGTGTAT GATCACTGTA CTCAGTCATG GCCTCAGGAG CGGTGGAGGA 
GTTGGAGATG TCCTGCGCAA ACCGTCTAAA GAGGAGCGTC TGTTCGCCGC ACGGGTGGTG 
TACGATCTCC TGTTCTTCTT CCTGGTGATC ATCATTGTGT TGAATCTGAT CTTCGGTGTG 
ATCATTGACA CATTTGCAGA TCTGAGAAGC GAGAAGCAGA GAAAAGAGGA GATCTTGAAG 
ACCACATGCT TCATCTGTGG TCTGGAGAGA GATAAGTTTG ATAATAAAAC GGTGACGTTT 
GAAGAGCACA TCAAGGAGGA GCACAACCTG TGGCATTACC TCTTCTTCAT AGTGCTGGTG 
CGGGTCAAAG ACTCCACCGA ATACACGGGA CCCGAGAGCT ACGTCGCTCA GATGATCAAG 
GAGCACAATC TGGACTGGTT TCCTCGTATG AGGGCGATGT CTCTGGTCAG CAGTGATGGA 
GAAGGTGAAC AGAATGAACT GCGCAGCTTA CAGGAGAAAC TCGAGTCCAC AATGAGGCTC 
GTCTCTAATC TTACCAATCA GCTCACAGAG CTCAAAGAGC AGATGACTGA GCAAAGGAAA 
CACAAGCAGA GGCTCGGTCT GCTGGGAAAC CCCGCCCACC TCAACATCAA CCCACAGCAG 
CCAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212154.1
CDS	148..8196
Translation	MVDKLSSFLHIGDVCSLYAEGSTSGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGTSTTDTVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDAAGNEGSWFYIQPFYKLRSIGDNVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCTTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDEHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEVNPDYEEECLESRSSMDSEHEVIRARARNPQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSPLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAAKLEKGTITQNERRSVTKLLEDLVYFVVDIPSSAQDVLEITVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRYSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDPANADILIETKLVLSRFEVAGTVLGEGAEEEEEDEEEVWLFWKDSGGEVKSKSIRELAQDAKDGHTEDQEVINYYRYQLNLFARMCLDRQYLAINKISAQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSQISIDDYDNDGTSSDEVKERFSQTMEFVENYLRDVVCQSFPFSDKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHISTPFHIKLDREPGKGSNVMRSIHGVGELMTQVVLRGGGFLPASSHNPPDRDEVKAQSEPQKQDILVMDTKLKIIEILQFILNVRLDYRISCLLCIFKTEFDESNSQSEPSLSPESPASVQGALDFEHIEEQAEGIFGGSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALHLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSDSGLDAGEVTPEAHHKGVINSSRSNKPKVESTSSSNYRVVKEILLRLSKLCVLEGISGKKNKKQQQRLLRNMGAHSVVLELLQIPYEKGEDVQMQEIMTLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAVTMQHIFMNNFQLCSEINERVVQHFVHCIETHGRSVHYLKFLQTIVKAENKFIKKCQDIVMAELVTAGEDVLVFYNDRASFQSLVQMMRLERERLDESSALRYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKMAYVNFLNHCYVDTEVEMKEIYTSNHMWKLFDDFLVDVCRVCNNTSDRKHADTVLERYVTETIMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQGVFRVYHCNWLLPGQKGNVEACIKVLSDVAKGRAIAIPVDLDCQVNNLFMKSNNIVQKTALSWRLSVRNATRRDSVLTTSRDYRNIIERLQDIVSALEERLRPLVQAELSVLVDVLHRPELLFPEHTEAHRKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKGEALRQLLVNRYYGFRSGGRRESLTSFGNSTLTPVGPIKSQPGGLSRAEMSLMEVQCHLDREGASDLVIDLIMNTTSDRVFHESILLAIALLEGGNTIIQHSFFKRLTEDKNSEKFFRVFYDRMKVAQVEIKATVTVNTSDLGNKRRDDNTPDKDTPQRRRGKDSGVVVTDDAREQLLEASAATKKAFGSYRRDADPEETFGTADGDKGGGDKGTEQGEMSPVILIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINQHNVALINQTVESLTEYCQGPCHDNQNCIATHESNGIDIIIALILNDINPLGRKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVMKMAYQQGEAEFEDEEQENGEDHAASPRNVGHNIYILAHQLSRHNKELQILLKPGGEDQALEYYTKHTSQIEIVRQDRTMEQIVFPVPNICSFLTNESKLRVYYGTERDEQGSKINDFFLHADDLFNEMRWQKKLRAQPVLYWCSRNMSFWSNVSFNLAVLINLLVAFFYPLDGVSESQLEPSLSLLLWVCLLGSLGFVLMSPRPNAVRVLVISTVLQLGFSVGLQHMLTLLGAFNVCNKIVFMLSFVGNRGSFTRGYRVMVMDREFLFHLLYLLICILGLFGHVFFYSLLLFDLVNREETLLNVIKSVTRNGRSIVLTAVLGLILVYLFSIVGYMFFKDDFILEVNRISNGTLEEGVTLPAGFCDARNGSCLSDDVDEDDVERACDSLWMCMITVLSHGLRSGGGVGDVLRKPSKEERLFAARVVYDLLFFFLVIIIVLNLIFGVIIDTFADLRSEKQRKEEILKTTCFICGLERDKFDNKTVTFEEHIKEEHNLWHYLFFIVLVRVKDSTEYTGPESYVAQMIKEHNLDWFPRMRAMSLVSSDGEGEQNELRSLQEKLESTMRLVSNLTNQLTELKEQMTEQRKHKQRLGLLGNPAHLNINPQQPA

Target ORF information:

RefSeq Version	XM_017356665.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356665.2

ORF Insert Sequence:

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GGTAAATCCA 
GACTATGAAG AAGAATGCCT GGAGTCCCGC TCCTCTATGG ATTCGGAGCA TGAAGTGATT 
CGGGCTCGTG CACGTAACCC TCAGGATAAG GTGATGTACA CACTGGTGTC TGTTCCTGAT 
GGAAATGACA TATCCTCCAT CTTTGAGCTT GACCCTACAA CTTTGAGGGG CGGAGACTCA 
CTTGTGCCAA GAAACTCGTA TGTGAGGCTC AGGCATCTCT GCACCAACAC GTGGGTCCAC 
AGCACCAACC AGCCAATAGA TAAAGAGGAA GAGAAACCGG TCATGCTCAG AATAGGAACG 
TCTCCTCTGA AGGAAGATAA AGAGGCGTTT GCTATAGTAC CAGTCTCTCC AGCTGAGGTT 
CGAGATCTGG ATTTTGCTAA TGATGCTAGT AAAGTGTTGG CTTCCATTGC TGCCAAACTG 
GAGAAGGGCA CCATTACCCA GAATGAGAGG AGGTCAGTGA CGAAGCTGTT GGAGGATCTG 
GTGTATTTTG TGGTGGACAT ACCCAGCAGT GCTCAAGATG TGCTGGAGAT CACAGTCAAC 
AAACCCAACA GAGAGCGACA GAAACTCATG AGAGAGCAGA ACATTCTTAA ACAGATCTTC 
AAGCTGCTGC AGGCGCCATT TACAGACAGC GGGGACGGCC CTATGTTGCG ATTAGAAGAG 
CTCGCAGACC AGCGTCATGC ACCCTTTCGA CACATTTGCA GACTCTGTTA CAGAGTCCTA 
CGTTACTCAC AACAGGACTA TCGCAAAAAT CAGGAGTACA TAGCAAAGCA GTTCCGCTTC 
ATGCAGAAGC AGATAGGATA TGATGTGCTG GCTGAAGACA CAATTACAGC CCTTCTGCAC 
AACAACAGGA AGCTCCTAGA GAAACACATC ACAGCAGCTG AAATAGATAC CTTTGTCAGT 
CTGGTCCGCA AGAACAGAGA GCCCAGATTT CTGGATTACT TGTCAGATCT GTGTGTGTCG 
ATGAATAAAT CCATCCCGGT CACTCAAGAG CTCATCTGTA ATGCTGTGCT GGACCCAGCC 
AATGCAGACA TTCTCATCGA AACCAAGCTT GTGTTGTCAC GGTTTGAGGT GGCAGGAACG 
GTGTTGGGTG AAGGTGCGGA GGAGGAAGAG GAGGATGAAG AGGAGGTCTG GCTGTTCTGG 
AAGGACAGTG GAGGAGAGGT GAAGAGTAAA AGCATACGAG AGCTTGCACA GGACGCAAAA 
GATGGACATA CAGAGGACCA GGAAGTCATC AATTATTACA GATATCAGCT GAATTTGTTT 
GCTCGGATGT GTCTGGATCG ACAGTATCTT GCGATTAACA AGATATCAGC ACAGCTGGAT 
GTGGATCTGA TTCTGCGCTG CATGTCAGAT GAAGATTTGC CCTTTGACCT CAGAGCATCG 
TTCTGCCGCA TGATGCTGCA CATGCATGTA GACCGTGACC CTCAGGAGCA GGTCACCCCG 
GTCAAATATG CTCGACTGTG GTCTGAAATC CCATCACAAA TTTCCATTGA TGATTATGAT 
AATGATGGGA CGTCAAGTGA TGAAGTGAAA GAGCGCTTCT CTCAGACGAT GGAGTTTGTG 
GAGAATTACC TGAGGGATGT TGTCTGCCAG AGTTTCCCGT TCTCTGATAA GGAGAAAAAC 
AAACTCACTT TTGAGGTGGT GAACCTGGCA AGAAACCTGA TCTATTTTGG TTTCTATAAC 
TTCAGTGATC TGCTAAGGTT AACAAAAATC CTATTGGCAA TCCTGGACTG TGTCCACATA 
AGCACTCCCT TCCACATCAA GCTGGATAGA GAACCCGGTA AAGGAAGTAA CGTGATGCGC 
TCCATCCATG GGGTGGGAGA GCTGATGACA CAGGTGGTGT TGAGGGGTGG GGGATTTCTG 
CCAGCCTCCA GCCACAATCC TCCTGACAGA GACGAAGTGA AAGCACAAAG TGAACCTCAG 
AAACAGGACA TACTGGTGAT GGACACTAAA CTCAAGATCA TCGAGATCCT GCAGTTTATT 
CTGAATGTGC GGTTGGACTA CAGGATCTCC TGCCTCCTCT GTATCTTTAA GACAGAGTTT 
GATGAAAGTA ACTCTCAAAG TGAACCATCT TTAAGCCCAG AATCTCCTGC CAGCGTTCAA 
GGTGCGCTAG ACTTTGAGCA CATTGAAGAG CAGGCGGAGG GTATTTTTGG AGGAAGTGAA 
GAGAACACCC CGCTGGATCT TGATGATCAT GGTGGACGGA CATTTCTGAG GGTCCTGCTT 
CACCTGACCA TGCATGACTA TCCTCCGCTG GTATCTCGAG CTCTTCACCT GCTCTTCAGA 
CACTTTAGCC AACGCCAGGA GGTCCTACAG GCCTTCAAAC AGGTTCAGCT CCTGGTGACC 
AGTCAGGATG TGGAAAACTA TAAACAGATC AAGTCAGATT TGGACCAGCT GAGGTCAATA 
GTAGAGAAAT CAGAGTTGTG GGTGTATAAG AGACAAGGCT CAGACTCAGG ACTGGATGCT 
GGAGAAGTCA CCCCTGAAGC CCACCACAAG GGTGTCATCA ACTCGAGTAG ATCAAACAAA 
CCCAAAGTGG AAAGTACCAG CAGCTCGAAC TACAGAGTCG TGAAAGAGAT CCTGCTGAGG 
CTGAGCAAAC TGTGTGTTTT GGAGGGCATC TCTGGCAAGA AGAATAAGAA ACAGCAGCAG 
CGGCTCCTCA GGAACATGGG AGCTCACAGT GTGGTCCTCG AACTGTTGCA AATCCCTTAT 
GAGAAGGGGG AGGATGTCCA GATGCAGGAG ATCATGACCT TAGCACATCA GTTCCTTCAA 
AACTTCTGTG CTGGAAATCA GCAGAACCAG GCACTTCTAC ACAAACACAT CAACCTGTTC 
CTCAACCCTG GGATCCTGGA GGCGGTGACC ATGCAGCATA TCTTCATGAA TAATTTCCAG 
TTGTGTAGTG AGATTAATGA GCGGGTCGTG CAGCATTTTG TGCACTGCAT CGAGACACAC 
GGCCGCAGCG TGCACTACCT CAAGTTCCTG CAGACCATTG TCAAGGCAGA GAACAAGTTC 
ATCAAGAAAT GCCAAGACAT CGTCATGGCT GAGCTGGTGA CTGCAGGTGA GGATGTGCTG 
GTGTTCTATA ATGACCGGGC GTCTTTTCAG TCATTGGTTC AGATGATGCG TCTTGAGCGG 
GAACGACTTG ATGAGAGCAG TGCACTCAGG TATCACATTC ACCTGGTGGA GTTGCTGGCT 
GTGTGCACTG AAGGCAAGAA CGTCTACACT GAGATCAAAT GCAACTCGCT GCTCCCTCTC 
GATGATATTG TACGTGTGGT CACACATGAG GACTGCATCC CTGAGGTGAA GATGGCCTAC 
GTGAACTTCC TGAATCACTG TTATGTTGAT ACAGAGGTTG AGATGAAGGA GATCTACACC 
AGCAATCACA TGTGGAAACT TTTTGATGAT TTTCTGGTGG ACGTCTGCAG AGTGTGCAAT 
AACACAAGTG ACCGTAAACA CGCTGACACA GTTCTGGAGA GATACGTTAC AGAGACCATA 
ATGAGCATCG TCACCACCTT TTTCAGCTCA CCATTCTCAG ACCAGAGCAC AAGTTTGCAG 
ACCCGGCAGC CGGTGTTTGT GCAGCTGCTG CAGGGAGTGT TTCGTGTATA TCACTGCAAC 
TGGCTTCTCC CAGGTCAGAA GGGGAATGTG GAAGCCTGCA TCAAAGTGCT GAGTGACGTG 
GCCAAAGGCA GAGCCATAGC CATTCCTGTG GATCTGGACT GTCAGGTGAA TAATCTGTTC 
ATGAAGTCCA ACAACATTGT GCAGAAAACA GCCCTCAGCT GGAGACTCTC TGTCCGTAAC 
GCGACTCGGA GAGACTCTGT TCTGACCACC TCTCGAGACT ATCGCAACAT CATTGAAAGA 
CTACAGGATA TAGTGTCTGC ATTGGAGGAG CGGTTGAGGC CTCTGGTTCA GGCTGAGTTG 
TCAGTGTTGG TGGATGTTCT CCATCGCCCA GAGCTGCTGT TTCCAGAGCA CACTGAAGCC 
CACCGCAAGT GTGAGAGTGG AGGCTTCATC TGCAAGTTGA TAAAACACAC TAAGCAGCTT 
CTAGAAGAAA ACGAAGAGAG GCTTTGCATC AAAGTCCTTC AGACACTCCG AGAGATGATG 
ACCAAAGACA GAGGATATGG AGAGAAGGGG GAGGCCCTTC GGCAGCTGCT CGTCAATCGT 
TACTATGGCT TCAGGAGTGG TGGGCGGAGA GAAAGTCTGA CCTCGTTTGG TAACTCCACC 
CTAACTCCTG TCGGGCCAAT CAAGAGCCAG CCAGGGGGGT TGAGCAGGGC TGAGATGTCA 
TTAATGGAGG TGCAGTGTCA TCTAGACCGT GAAGGGGCAT CGGATCTTGT CATTGATCTC 
ATCATGAACA CTACCAGCGA CCGCGTCTTC CATGAAAGCA TTCTGTTGGC CATCGCACTG 
TTGGAGGGCG GAAACACCAT CATACAGCAT TCCTTCTTCA AACGTTTAAC AGAAGACAAA 
AATTCAGAGA AGTTTTTCAG GGTGTTTTAT GACAGAATGA AAGTGGCTCA GGTGGAGATC 
AAAGCCACGG TTACTGTCAA CACTAGTGAC CTCGGCAACA AGCGGCGTGA CGACAACACT 
CCAGACAAAG ATACTCCACA ACGCCGCAGA GGTAAAGACT CCGGTGTGGT GGTGACAGAT 
GATGCTCGTG AGCAGCTGTT GGAGGCTTCA GCTGCGACTA AGAAGGCCTT TGGTTCATAT 
CGTCGAGATG CTGATCCCGA GGAGACGTTT GGCACGGCTG ATGGAGATAA AGGAGGTGGA 
GATAAAGGCA CAGAGCAGGG CGAGATGAGC CCTGTCATCC TCATAATGCA GCCAATCCTG 
CGCTTCCTGC AGCTGCTGTG CGAAAACCAC AATCGAGACC TGCAGAATTT TCTGCGCTGT 
CAGAACAATA AAAACAACTA TAATCTGGTG TGTGAGACGC TGCAGTTCCT GGACTGTATC 
TGTGGCAGCA CAACAGGAGG CCTGGGGCTG TTAGGACTCT ACATCAACCA GCATAACGTA 
GCGCTCATTA ATCAGACTGT AGAGAGCCTC ACTGAATACT GTCAAGGTCC CTGCCATGAC 
AATCAGAATT GCATTGCCAC CCATGAGTCT AATGGCATCG ACATCATTAT TGCACTGATT 
CTGAATGATA TTAATCCACT GGGCAGGAAG AGAATGGATC TGGTTCTAGA ACTAAAGAAT 
AATGCATCTA AGCTGCTGCT GGCCATCATG GAGAGCCGTC ATGACAGTGA GAATGCAGAA 
AGAATTCTGT ACAACATGAG ACCAAAAGAA CTAGTGGAGG TGATGAAAAT GGCCTACCAG 
CAGGGGGAGG CAGAGTTTGA GGATGAGGAA CAGGAGAATG GAGAGGATCA TGCAGCATCG 
CCTCGCAATG TTGGACACAA CATCTATATT CTTGCACATC AGCTGTCGCG TCACAATAAG 
GAATTGCAAA TACTACTGAA ACCAGGTGGA GAGGACCAGG CCCTGGAGTA TTACACAAAA 
CACACCTCAC AGATAGAGAT AGTTCGACAG GATCGCACTA TGGAACAGAT TGTGTTTCCT 
GTACCAAATA TCTGCTCTTT TCTGACAAAT GAGTCCAAGC TGCGTGTGTA CTATGGTACA 
GAGAGAGACG AGCAAGGCAG TAAAATAAAT GACTTCTTCT TGCATGCAGA CGACCTCTTT 
AATGAAATGA GGTGGCAGAA AAAACTAAGA GCTCAGCCAG TGCTGTACTG GTGCAGCAGG 
AACATGTCGT TTTGGAGTAA CGTTTCCTTT AATCTGGCGG TGCTCATTAA TTTGCTGGTG 
GCATTTTTCT ACCCTCTGGA TGGAGTGAGC GAAAGCCAGC TGGAACCTTC TCTGTCTCTG 
TTGCTGTGGG TTTGTTTGTT GGGCTCACTG GGCTTCGTGT TGATGTCCCC GAGGCCAAAT 
GCAGTGCGGG TGCTGGTCAT CTCCACTGTC CTGCAGTTGG GCTTCTCTGT AGGGCTGCAG 
CACATGCTCA CACTCCTGGG AGCTTTCAAT GTTTGTAATA AGATCGTGTT CATGCTGAGC 
TTTGTGGGTA ACCGTGGGTC ATTCACCCGG GGTTATCGGG TGATGGTCAT GGATAGAGAG 
TTCCTGTTTC ATCTGCTGTA CCTTCTCATC TGCATACTGG GCCTTTTTGG ACACGTCTTC 
TTTTACAGTC TTCTGTTGTT TGACCTGGTG AACCGGGAAG AGACTCTTCT GAACGTCATC 
AAGAGTGTGA CTCGAAACGG CCGCTCCATC GTCTTGACCG CAGTGCTGGG CCTCATCCTG 
GTCTACCTCT TTTCTATTGT TGGATATATG TTTTTCAAAG ATGACTTCAT CCTGGAGGTG 
AACCGCATCT CCAACGGCAC TCTGGAGGAG GGGGTGACTT TACCTGCTGG TTTTTGTGAT 
GCGAGAAACG GGTCGTGTCT TTCTGATGAT GTTGACGAGG ATGACGTGGA GCGCGCGTGT 
GATTCTCTAT GGATGTGTAT GATCACTGTA CTCAGTCATG GCCTCAGGAG CGGTGGAGGA 
GTTGGAGATG TCCTGCGCAA ACCGTCTAAA GAGGAGCGTC TGTTCGCCGC ACGGGTGGTG 
TACGATCTCC TGTTCTTCTT CCTGGTGATC ATCATTGTGT TGAATCTGAT CTTCGGTGTG 
ATCATTGACA CATTTGCAGA TCTGAGAAGC GAGAAGCAGA GAAAAGAGGA GATCTTGAAG 
ACCACATGCT TCATCTGTGG TCTGGAGAGA GATAAGTTTG ATAATAAAAC GGTGACGTTT 
GAAGAGCACA TCAAGGAGGA GCACAACCTG TGGCATTACC TCTTCTTCAT AGTGCTGGTG 
CGGGTCAAAG ACTCCACCGA ATACACGGGA CCCGAGAGCT ACGTCGCTCA GATGATCAAG 
GAGCACAATC TGGACTGGTT TCCTCGTATG AGGGCGATGT CTCTGGTCAG CAGTGATGGA 
GAAGGTGAAC AGAATGAACT GCGCAGCTTA CAGGAGAAAC TCGAGTCCAC AATGAGGCTC 
GTCTCTAATC TTACCAATCA GCTCACAGAG CTCAAAGAGC AGATGACTGA GCAAAGGAAA 
CACAAGCAGA GGCTCGGTCT GCTGGGAAAC CCCGCCCACC TCAACATCAA CCCACAGCAG 
CCAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itpr1a ( XM_017356665.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212154.1 Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X6, mRNA.

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CloneID	ODa59494
Clone ID Related Accession (Same CDS sequence)	XM_017356665.2 , XM_017356665.1
Accession Version	XM_017356665.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GGTAAATCCA 
GACTATGAAG AAGAATGCCT GGAGTCCCGC TCCTCTATGG ATTCGGAGCA TGAAGTGATT 
CGGGCTCGTG CACGTAACCC TCAGGATAAG GTGATGTACA CACTGGTGTC TGTTCCTGAT 
GGAAATGACA TATCCTCCAT CTTTGAGCTT GACCCTACAA CTTTGAGGGG CGGAGACTCA 
CTTGTGCCAA GAAACTCGTA TGTGAGGCTC AGGCATCTCT GCACCAACAC GTGGGTCCAC 
AGCACCAACC AGCCAATAGA TAAAGAGGAA GAGAAACCGG TCATGCTCAG AATAGGAACG 
TCTCCTCTGA AGGAAGATAA AGAGGCGTTT GCTATAGTAC CAGTCTCTCC AGCTGAGGTT 
CGAGATCTGG ATTTTGCTAA TGATGCTAGT AAAGTGTTGG CTTCCATTGC TGCCAAACTG 
GAGAAGGGCA CCATTACCCA GAATGAGAGG AGGTCAGTGA CGAAGCTGTT GGAGGATCTG 
GTGTATTTTG TGGTGGACAT ACCCAGCAGT GCTCAAGATG TGCTGGAGAT CACAGTCAAC 
AAACCCAACA GAGAGCGACA GAAACTCATG AGAGAGCAGA ACATTCTTAA ACAGATCTTC 
AAGCTGCTGC AGGCGCCATT TACAGACAGC GGGGACGGCC CTATGTTGCG ATTAGAAGAG 
CTCGCAGACC AGCGTCATGC ACCCTTTCGA CACATTTGCA GACTCTGTTA CAGAGTCCTA 
CGTTACTCAC AACAGGACTA TCGCAAAAAT CAGGAGTACA TAGCAAAGCA GTTCCGCTTC 
ATGCAGAAGC AGATAGGATA TGATGTGCTG GCTGAAGACA CAATTACAGC CCTTCTGCAC 
AACAACAGGA AGCTCCTAGA GAAACACATC ACAGCAGCTG AAATAGATAC CTTTGTCAGT 
CTGGTCCGCA AGAACAGAGA GCCCAGATTT CTGGATTACT TGTCAGATCT GTGTGTGTCG 
ATGAATAAAT CCATCCCGGT CACTCAAGAG CTCATCTGTA ATGCTGTGCT GGACCCAGCC 
AATGCAGACA TTCTCATCGA AACCAAGCTT GTGTTGTCAC GGTTTGAGGT GGCAGGAACG 
GTGTTGGGTG AAGGTGCGGA GGAGGAAGAG GAGGATGAAG AGGAGGTCTG GCTGTTCTGG 
AAGGACAGTG GAGGAGAGGT GAAGAGTAAA AGCATACGAG AGCTTGCACA GGACGCAAAA 
GATGGACATA CAGAGGACCA GGAAGTCATC AATTATTACA GATATCAGCT GAATTTGTTT 
GCTCGGATGT GTCTGGATCG ACAGTATCTT GCGATTAACA AGATATCAGC ACAGCTGGAT 
GTGGATCTGA TTCTGCGCTG CATGTCAGAT GAAGATTTGC CCTTTGACCT CAGAGCATCG 
TTCTGCCGCA TGATGCTGCA CATGCATGTA GACCGTGACC CTCAGGAGCA GGTCACCCCG 
GTCAAATATG CTCGACTGTG GTCTGAAATC CCATCACAAA TTTCCATTGA TGATTATGAT 
AATGATGGGA CGTCAAGTGA TGAAGTGAAA GAGCGCTTCT CTCAGACGAT GGAGTTTGTG 
GAGAATTACC TGAGGGATGT TGTCTGCCAG AGTTTCCCGT TCTCTGATAA GGAGAAAAAC 
AAACTCACTT TTGAGGTGGT GAACCTGGCA AGAAACCTGA TCTATTTTGG TTTCTATAAC 
TTCAGTGATC TGCTAAGGTT AACAAAAATC CTATTGGCAA TCCTGGACTG TGTCCACATA 
AGCACTCCCT TCCACATCAA GCTGGATAGA GAACCCGGTA AAGGAAGTAA CGTGATGCGC 
TCCATCCATG GGGTGGGAGA GCTGATGACA CAGGTGGTGT TGAGGGGTGG GGGATTTCTG 
CCAGCCTCCA GCCACAATCC TCCTGACAGA GACGAAGTGA AAGCACAAAG TGAACCTCAG 
AAACAGGACA TACTGGTGAT GGACACTAAA CTCAAGATCA TCGAGATCCT GCAGTTTATT 
CTGAATGTGC GGTTGGACTA CAGGATCTCC TGCCTCCTCT GTATCTTTAA GACAGAGTTT 
GATGAAAGTA ACTCTCAAAG TGAACCATCT TTAAGCCCAG AATCTCCTGC CAGCGTTCAA 
GGTGCGCTAG ACTTTGAGCA CATTGAAGAG CAGGCGGAGG GTATTTTTGG AGGAAGTGAA 
GAGAACACCC CGCTGGATCT TGATGATCAT GGTGGACGGA CATTTCTGAG GGTCCTGCTT 
CACCTGACCA TGCATGACTA TCCTCCGCTG GTATCTCGAG CTCTTCACCT GCTCTTCAGA 
CACTTTAGCC AACGCCAGGA GGTCCTACAG GCCTTCAAAC AGGTTCAGCT CCTGGTGACC 
AGTCAGGATG TGGAAAACTA TAAACAGATC AAGTCAGATT TGGACCAGCT GAGGTCAATA 
GTAGAGAAAT CAGAGTTGTG GGTGTATAAG AGACAAGGCT CAGACTCAGG ACTGGATGCT 
GGAGAAGTCA CCCCTGAAGC CCACCACAAG GGTGTCATCA ACTCGAGTAG ATCAAACAAA 
CCCAAAGTGG AAAGTACCAG CAGCTCGAAC TACAGAGTCG TGAAAGAGAT CCTGCTGAGG 
CTGAGCAAAC TGTGTGTTTT GGAGGGCATC TCTGGCAAGA AGAATAAGAA ACAGCAGCAG 
CGGCTCCTCA GGAACATGGG AGCTCACAGT GTGGTCCTCG AACTGTTGCA AATCCCTTAT 
GAGAAGGGGG AGGATGTCCA GATGCAGGAG ATCATGACCT TAGCACATCA GTTCCTTCAA 
AACTTCTGTG CTGGAAATCA GCAGAACCAG GCACTTCTAC ACAAACACAT CAACCTGTTC 
CTCAACCCTG GGATCCTGGA GGCGGTGACC ATGCAGCATA TCTTCATGAA TAATTTCCAG 
TTGTGTAGTG AGATTAATGA GCGGGTCGTG CAGCATTTTG TGCACTGCAT CGAGACACAC 
GGCCGCAGCG TGCACTACCT CAAGTTCCTG CAGACCATTG TCAAGGCAGA GAACAAGTTC 
ATCAAGAAAT GCCAAGACAT CGTCATGGCT GAGCTGGTGA CTGCAGGTGA GGATGTGCTG 
GTGTTCTATA ATGACCGGGC GTCTTTTCAG TCATTGGTTC AGATGATGCG TCTTGAGCGG 
GAACGACTTG ATGAGAGCAG TGCACTCAGG TATCACATTC ACCTGGTGGA GTTGCTGGCT 
GTGTGCACTG AAGGCAAGAA CGTCTACACT GAGATCAAAT GCAACTCGCT GCTCCCTCTC 
GATGATATTG TACGTGTGGT CACACATGAG GACTGCATCC CTGAGGTGAA GATGGCCTAC 
GTGAACTTCC TGAATCACTG TTATGTTGAT ACAGAGGTTG AGATGAAGGA GATCTACACC 
AGCAATCACA TGTGGAAACT TTTTGATGAT TTTCTGGTGG ACGTCTGCAG AGTGTGCAAT 
AACACAAGTG ACCGTAAACA CGCTGACACA GTTCTGGAGA GATACGTTAC AGAGACCATA 
ATGAGCATCG TCACCACCTT TTTCAGCTCA CCATTCTCAG ACCAGAGCAC AAGTTTGCAG 
ACCCGGCAGC CGGTGTTTGT GCAGCTGCTG CAGGGAGTGT TTCGTGTATA TCACTGCAAC 
TGGCTTCTCC CAGGTCAGAA GGGGAATGTG GAAGCCTGCA TCAAAGTGCT GAGTGACGTG 
GCCAAAGGCA GAGCCATAGC CATTCCTGTG GATCTGGACT GTCAGGTGAA TAATCTGTTC 
ATGAAGTCCA ACAACATTGT GCAGAAAACA GCCCTCAGCT GGAGACTCTC TGTCCGTAAC 
GCGACTCGGA GAGACTCTGT TCTGACCACC TCTCGAGACT ATCGCAACAT CATTGAAAGA 
CTACAGGATA TAGTGTCTGC ATTGGAGGAG CGGTTGAGGC CTCTGGTTCA GGCTGAGTTG 
TCAGTGTTGG TGGATGTTCT CCATCGCCCA GAGCTGCTGT TTCCAGAGCA CACTGAAGCC 
CACCGCAAGT GTGAGAGTGG AGGCTTCATC TGCAAGTTGA TAAAACACAC TAAGCAGCTT 
CTAGAAGAAA ACGAAGAGAG GCTTTGCATC AAAGTCCTTC AGACACTCCG AGAGATGATG 
ACCAAAGACA GAGGATATGG AGAGAAGGGG GAGGCCCTTC GGCAGCTGCT CGTCAATCGT 
TACTATGGCT TCAGGAGTGG TGGGCGGAGA GAAAGTCTGA CCTCGTTTGG TAACTCCACC 
CTAACTCCTG TCGGGCCAAT CAAGAGCCAG CCAGGGGGGT TGAGCAGGGC TGAGATGTCA 
TTAATGGAGG TGCAGTGTCA TCTAGACCGT GAAGGGGCAT CGGATCTTGT CATTGATCTC 
ATCATGAACA CTACCAGCGA CCGCGTCTTC CATGAAAGCA TTCTGTTGGC CATCGCACTG 
TTGGAGGGCG GAAACACCAT CATACAGCAT TCCTTCTTCA AACGTTTAAC AGAAGACAAA 
AATTCAGAGA AGTTTTTCAG GGTGTTTTAT GACAGAATGA AAGTGGCTCA GGTGGAGATC 
AAAGCCACGG TTACTGTCAA CACTAGTGAC CTCGGCAACA AGCGGCGTGA CGACAACACT 
CCAGACAAAG ATACTCCACA ACGCCGCAGA GGTAAAGACT CCGGTGTGGT GGTGACAGAT 
GATGCTCGTG AGCAGCTGTT GGAGGCTTCA GCTGCGACTA AGAAGGCCTT TGGTTCATAT 
CGTCGAGATG CTGATCCCGA GGAGACGTTT GGCACGGCTG ATGGAGATAA AGGAGGTGGA 
GATAAAGGCA CAGAGCAGGG CGAGATGAGC CCTGTCATCC TCATAATGCA GCCAATCCTG 
CGCTTCCTGC AGCTGCTGTG CGAAAACCAC AATCGAGACC TGCAGAATTT TCTGCGCTGT 
CAGAACAATA AAAACAACTA TAATCTGGTG TGTGAGACGC TGCAGTTCCT GGACTGTATC 
TGTGGCAGCA CAACAGGAGG CCTGGGGCTG TTAGGACTCT ACATCAACCA GCATAACGTA 
GCGCTCATTA ATCAGACTGT AGAGAGCCTC ACTGAATACT GTCAAGGTCC CTGCCATGAC 
AATCAGAATT GCATTGCCAC CCATGAGTCT AATGGCATCG ACATCATTAT TGCACTGATT 
CTGAATGATA TTAATCCACT GGGCAGGAAG AGAATGGATC TGGTTCTAGA ACTAAAGAAT 
AATGCATCTA AGCTGCTGCT GGCCATCATG GAGAGCCGTC ATGACAGTGA GAATGCAGAA 
AGAATTCTGT ACAACATGAG ACCAAAAGAA CTAGTGGAGG TGATGAAAAT GGCCTACCAG 
CAGGGGGAGG CAGAGTTTGA GGATGAGGAA CAGGAGAATG GAGAGGATCA TGCAGCATCG 
CCTCGCAATG TTGGACACAA CATCTATATT CTTGCACATC AGCTGTCGCG TCACAATAAG 
GAATTGCAAA TACTACTGAA ACCAGGTGGA GAGGACCAGG CCCTGGAGTA TTACACAAAA 
CACACCTCAC AGATAGAGAT AGTTCGACAG GATCGCACTA TGGAACAGAT TGTGTTTCCT 
GTACCAAATA TCTGCTCTTT TCTGACAAAT GAGTCCAAGC TGCGTGTGTA CTATGGTACA 
GAGAGAGACG AGCAAGGCAG TAAAATAAAT GACTTCTTCT TGCATGCAGA CGACCTCTTT 
AATGAAATGA GGTGGCAGAA AAAACTAAGA GCTCAGCCAG TGCTGTACTG GTGCAGCAGG 
AACATGTCGT TTTGGAGTAA CGTTTCCTTT AATCTGGCGG TGCTCATTAA TTTGCTGGTG 
GCATTTTTCT ACCCTCTGGA TGGAGTGAGC GAAAGCCAGC TGGAACCTTC TCTGTCTCTG 
TTGCTGTGGG TTTGTTTGTT GGGCTCACTG GGCTTCGTGT TGATGTCCCC GAGGCCAAAT 
GCAGTGCGGG TGCTGGTCAT CTCCACTGTC CTGCAGTTGG GCTTCTCTGT AGGGCTGCAG 
CACATGCTCA CACTCCTGGG AGCTTTCAAT GTTTGTAATA AGATCGTGTT CATGCTGAGC 
TTTGTGGGTA ACCGTGGGTC ATTCACCCGG GGTTATCGGG TGATGGTCAT GGATAGAGAG 
TTCCTGTTTC ATCTGCTGTA CCTTCTCATC TGCATACTGG GCCTTTTTGG ACACGTCTTC 
TTTTACAGTC TTCTGTTGTT TGACCTGGTG AACCGGGAAG AGACTCTTCT GAACGTCATC 
AAGAGTGTGA CTCGAAACGG CCGCTCCATC GTCTTGACCG CAGTGCTGGG CCTCATCCTG 
GTCTACCTCT TTTCTATTGT TGGATATATG TTTTTCAAAG ATGACTTCAT CCTGGAGGTG 
AACCGCATCT CCAACGGCAC TCTGGAGGAG GGGGTGACTT TACCTGCTGG TTTTTGTGAT 
GCGAGAAACG GGTCGTGTCT TTCTGATGAT GTTGACGAGG ATGACGTGGA GCGCGCGTGT 
GATTCTCTAT GGATGTGTAT GATCACTGTA CTCAGTCATG GCCTCAGGAG CGGTGGAGGA 
GTTGGAGATG TCCTGCGCAA ACCGTCTAAA GAGGAGCGTC TGTTCGCCGC ACGGGTGGTG 
TACGATCTCC TGTTCTTCTT CCTGGTGATC ATCATTGTGT TGAATCTGAT CTTCGGTGTG 
ATCATTGACA CATTTGCAGA TCTGAGAAGC GAGAAGCAGA GAAAAGAGGA GATCTTGAAG 
ACCACATGCT TCATCTGTGG TCTGGAGAGA GATAAGTTTG ATAATAAAAC GGTGACGTTT 
GAAGAGCACA TCAAGGAGGA GCACAACCTG TGGCATTACC TCTTCTTCAT AGTGCTGGTG 
CGGGTCAAAG ACTCCACCGA ATACACGGGA CCCGAGAGCT ACGTCGCTCA GATGATCAAG 
GAGCACAATC TGGACTGGTT TCCTCGTATG AGGGCGATGT CTCTGGTCAG CAGTGATGGA 
GAAGGTGAAC AGAATGAACT GCGCAGCTTA CAGGAGAAAC TCGAGTCCAC AATGAGGCTC 
GTCTCTAATC TTACCAATCA GCTCACAGAG CTCAAAGAGC AGATGACTGA GCAAAGGAAA 
CACAAGCAGA GGCTCGGTCT GCTGGGAAAC CCCGCCCACC TCAACATCAA CCCACAGCAG 
CCAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212154.1
CDS	148..8196
Translation	MVDKLSSFLHIGDVCSLYAEGSTSGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGTSTTDTVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDAAGNEGSWFYIQPFYKLRSIGDNVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCTTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDEHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEVNPDYEEECLESRSSMDSEHEVIRARARNPQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSPLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAAKLEKGTITQNERRSVTKLLEDLVYFVVDIPSSAQDVLEITVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRYSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDPANADILIETKLVLSRFEVAGTVLGEGAEEEEEDEEEVWLFWKDSGGEVKSKSIRELAQDAKDGHTEDQEVINYYRYQLNLFARMCLDRQYLAINKISAQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSQISIDDYDNDGTSSDEVKERFSQTMEFVENYLRDVVCQSFPFSDKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHISTPFHIKLDREPGKGSNVMRSIHGVGELMTQVVLRGGGFLPASSHNPPDRDEVKAQSEPQKQDILVMDTKLKIIEILQFILNVRLDYRISCLLCIFKTEFDESNSQSEPSLSPESPASVQGALDFEHIEEQAEGIFGGSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALHLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSDSGLDAGEVTPEAHHKGVINSSRSNKPKVESTSSSNYRVVKEILLRLSKLCVLEGISGKKNKKQQQRLLRNMGAHSVVLELLQIPYEKGEDVQMQEIMTLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAVTMQHIFMNNFQLCSEINERVVQHFVHCIETHGRSVHYLKFLQTIVKAENKFIKKCQDIVMAELVTAGEDVLVFYNDRASFQSLVQMMRLERERLDESSALRYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKMAYVNFLNHCYVDTEVEMKEIYTSNHMWKLFDDFLVDVCRVCNNTSDRKHADTVLERYVTETIMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQGVFRVYHCNWLLPGQKGNVEACIKVLSDVAKGRAIAIPVDLDCQVNNLFMKSNNIVQKTALSWRLSVRNATRRDSVLTTSRDYRNIIERLQDIVSALEERLRPLVQAELSVLVDVLHRPELLFPEHTEAHRKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKGEALRQLLVNRYYGFRSGGRRESLTSFGNSTLTPVGPIKSQPGGLSRAEMSLMEVQCHLDREGASDLVIDLIMNTTSDRVFHESILLAIALLEGGNTIIQHSFFKRLTEDKNSEKFFRVFYDRMKVAQVEIKATVTVNTSDLGNKRRDDNTPDKDTPQRRRGKDSGVVVTDDAREQLLEASAATKKAFGSYRRDADPEETFGTADGDKGGGDKGTEQGEMSPVILIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINQHNVALINQTVESLTEYCQGPCHDNQNCIATHESNGIDIIIALILNDINPLGRKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVMKMAYQQGEAEFEDEEQENGEDHAASPRNVGHNIYILAHQLSRHNKELQILLKPGGEDQALEYYTKHTSQIEIVRQDRTMEQIVFPVPNICSFLTNESKLRVYYGTERDEQGSKINDFFLHADDLFNEMRWQKKLRAQPVLYWCSRNMSFWSNVSFNLAVLINLLVAFFYPLDGVSESQLEPSLSLLLWVCLLGSLGFVLMSPRPNAVRVLVISTVLQLGFSVGLQHMLTLLGAFNVCNKIVFMLSFVGNRGSFTRGYRVMVMDREFLFHLLYLLICILGLFGHVFFYSLLLFDLVNREETLLNVIKSVTRNGRSIVLTAVLGLILVYLFSIVGYMFFKDDFILEVNRISNGTLEEGVTLPAGFCDARNGSCLSDDVDEDDVERACDSLWMCMITVLSHGLRSGGGVGDVLRKPSKEERLFAARVVYDLLFFFLVIIIVLNLIFGVIIDTFADLRSEKQRKEEILKTTCFICGLERDKFDNKTVTFEEHIKEEHNLWHYLFFIVLVRVKDSTEYTGPESYVAQMIKEHNLDWFPRMRAMSLVSSDGEGEQNELRSLQEKLESTMRLVSNLTNQLTELKEQMTEQRKHKQRLGLLGNPAHLNINPQQPA

Target ORF information:

RefSeq Version	XM_017356665.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356665.1

ORF Insert Sequence:

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GGTAAATCCA 
GACTATGAAG AAGAATGCCT GGAGTCCCGC TCCTCTATGG ATTCGGAGCA TGAAGTGATT 
CGGGCTCGTG CACGTAACCC TCAGGATAAG GTGATGTACA CACTGGTGTC TGTTCCTGAT 
GGAAATGACA TATCCTCCAT CTTTGAGCTT GACCCTACAA CTTTGAGGGG CGGAGACTCA 
CTTGTGCCAA GAAACTCGTA TGTGAGGCTC AGGCATCTCT GCACCAACAC GTGGGTCCAC 
AGCACCAACC AGCCAATAGA TAAAGAGGAA GAGAAACCGG TCATGCTCAG AATAGGAACG 
TCTCCTCTGA AGGAAGATAA AGAGGCGTTT GCTATAGTAC CAGTCTCTCC AGCTGAGGTT 
CGAGATCTGG ATTTTGCTAA TGATGCTAGT AAAGTGTTGG CTTCCATTGC TGCCAAACTG 
GAGAAGGGCA CCATTACCCA GAATGAGAGG AGGTCAGTGA CGAAGCTGTT GGAGGATCTG 
GTGTATTTTG TGGTGGACAT ACCCAGCAGT GCTCAAGATG TGCTGGAGAT CACAGTCAAC 
AAACCCAACA GAGAGCGACA GAAACTCATG AGAGAGCAGA ACATTCTTAA ACAGATCTTC 
AAGCTGCTGC AGGCGCCATT TACAGACAGC GGGGACGGCC CTATGTTGCG ATTAGAAGAG 
CTCGCAGACC AGCGTCATGC ACCCTTTCGA CACATTTGCA GACTCTGTTA CAGAGTCCTA 
CGTTACTCAC AACAGGACTA TCGCAAAAAT CAGGAGTACA TAGCAAAGCA GTTCCGCTTC 
ATGCAGAAGC AGATAGGATA TGATGTGCTG GCTGAAGACA CAATTACAGC CCTTCTGCAC 
AACAACAGGA AGCTCCTAGA GAAACACATC ACAGCAGCTG AAATAGATAC CTTTGTCAGT 
CTGGTCCGCA AGAACAGAGA GCCCAGATTT CTGGATTACT TGTCAGATCT GTGTGTGTCG 
ATGAATAAAT CCATCCCGGT CACTCAAGAG CTCATCTGTA ATGCTGTGCT GGACCCAGCC 
AATGCAGACA TTCTCATCGA AACCAAGCTT GTGTTGTCAC GGTTTGAGGT GGCAGGAACG 
GTGTTGGGTG AAGGTGCGGA GGAGGAAGAG GAGGATGAAG AGGAGGTCTG GCTGTTCTGG 
AAGGACAGTG GAGGAGAGGT GAAGAGTAAA AGCATACGAG AGCTTGCACA GGACGCAAAA 
GATGGACATA CAGAGGACCA GGAAGTCATC AATTATTACA GATATCAGCT GAATTTGTTT 
GCTCGGATGT GTCTGGATCG ACAGTATCTT GCGATTAACA AGATATCAGC ACAGCTGGAT 
GTGGATCTGA TTCTGCGCTG CATGTCAGAT GAAGATTTGC CCTTTGACCT CAGAGCATCG 
TTCTGCCGCA TGATGCTGCA CATGCATGTA GACCGTGACC CTCAGGAGCA GGTCACCCCG 
GTCAAATATG CTCGACTGTG GTCTGAAATC CCATCACAAA TTTCCATTGA TGATTATGAT 
AATGATGGGA CGTCAAGTGA TGAAGTGAAA GAGCGCTTCT CTCAGACGAT GGAGTTTGTG 
GAGAATTACC TGAGGGATGT TGTCTGCCAG AGTTTCCCGT TCTCTGATAA GGAGAAAAAC 
AAACTCACTT TTGAGGTGGT GAACCTGGCA AGAAACCTGA TCTATTTTGG TTTCTATAAC 
TTCAGTGATC TGCTAAGGTT AACAAAAATC CTATTGGCAA TCCTGGACTG TGTCCACATA 
AGCACTCCCT TCCACATCAA GCTGGATAGA GAACCCGGTA AAGGAAGTAA CGTGATGCGC 
TCCATCCATG GGGTGGGAGA GCTGATGACA CAGGTGGTGT TGAGGGGTGG GGGATTTCTG 
CCAGCCTCCA GCCACAATCC TCCTGACAGA GACGAAGTGA AAGCACAAAG TGAACCTCAG 
AAACAGGACA TACTGGTGAT GGACACTAAA CTCAAGATCA TCGAGATCCT GCAGTTTATT 
CTGAATGTGC GGTTGGACTA CAGGATCTCC TGCCTCCTCT GTATCTTTAA GACAGAGTTT 
GATGAAAGTA ACTCTCAAAG TGAACCATCT TTAAGCCCAG AATCTCCTGC CAGCGTTCAA 
GGTGCGCTAG ACTTTGAGCA CATTGAAGAG CAGGCGGAGG GTATTTTTGG AGGAAGTGAA 
GAGAACACCC CGCTGGATCT TGATGATCAT GGTGGACGGA CATTTCTGAG GGTCCTGCTT 
CACCTGACCA TGCATGACTA TCCTCCGCTG GTATCTCGAG CTCTTCACCT GCTCTTCAGA 
CACTTTAGCC AACGCCAGGA GGTCCTACAG GCCTTCAAAC AGGTTCAGCT CCTGGTGACC 
AGTCAGGATG TGGAAAACTA TAAACAGATC AAGTCAGATT TGGACCAGCT GAGGTCAATA 
GTAGAGAAAT CAGAGTTGTG GGTGTATAAG AGACAAGGCT CAGACTCAGG ACTGGATGCT 
GGAGAAGTCA CCCCTGAAGC CCACCACAAG GGTGTCATCA ACTCGAGTAG ATCAAACAAA 
CCCAAAGTGG AAAGTACCAG CAGCTCGAAC TACAGAGTCG TGAAAGAGAT CCTGCTGAGG 
CTGAGCAAAC TGTGTGTTTT GGAGGGCATC TCTGGCAAGA AGAATAAGAA ACAGCAGCAG 
CGGCTCCTCA GGAACATGGG AGCTCACAGT GTGGTCCTCG AACTGTTGCA AATCCCTTAT 
GAGAAGGGGG AGGATGTCCA GATGCAGGAG ATCATGACCT TAGCACATCA GTTCCTTCAA 
AACTTCTGTG CTGGAAATCA GCAGAACCAG GCACTTCTAC ACAAACACAT CAACCTGTTC 
CTCAACCCTG GGATCCTGGA GGCGGTGACC ATGCAGCATA TCTTCATGAA TAATTTCCAG 
TTGTGTAGTG AGATTAATGA GCGGGTCGTG CAGCATTTTG TGCACTGCAT CGAGACACAC 
GGCCGCAGCG TGCACTACCT CAAGTTCCTG CAGACCATTG TCAAGGCAGA GAACAAGTTC 
ATCAAGAAAT GCCAAGACAT CGTCATGGCT GAGCTGGTGA CTGCAGGTGA GGATGTGCTG 
GTGTTCTATA ATGACCGGGC GTCTTTTCAG TCATTGGTTC AGATGATGCG TCTTGAGCGG 
GAACGACTTG ATGAGAGCAG TGCACTCAGG TATCACATTC ACCTGGTGGA GTTGCTGGCT 
GTGTGCACTG AAGGCAAGAA CGTCTACACT GAGATCAAAT GCAACTCGCT GCTCCCTCTC 
GATGATATTG TACGTGTGGT CACACATGAG GACTGCATCC CTGAGGTGAA GATGGCCTAC 
GTGAACTTCC TGAATCACTG TTATGTTGAT ACAGAGGTTG AGATGAAGGA GATCTACACC 
AGCAATCACA TGTGGAAACT TTTTGATGAT TTTCTGGTGG ACGTCTGCAG AGTGTGCAAT 
AACACAAGTG ACCGTAAACA CGCTGACACA GTTCTGGAGA GATACGTTAC AGAGACCATA 
ATGAGCATCG TCACCACCTT TTTCAGCTCA CCATTCTCAG ACCAGAGCAC AAGTTTGCAG 
ACCCGGCAGC CGGTGTTTGT GCAGCTGCTG CAGGGAGTGT TTCGTGTATA TCACTGCAAC 
TGGCTTCTCC CAGGTCAGAA GGGGAATGTG GAAGCCTGCA TCAAAGTGCT GAGTGACGTG 
GCCAAAGGCA GAGCCATAGC CATTCCTGTG GATCTGGACT GTCAGGTGAA TAATCTGTTC 
ATGAAGTCCA ACAACATTGT GCAGAAAACA GCCCTCAGCT GGAGACTCTC TGTCCGTAAC 
GCGACTCGGA GAGACTCTGT TCTGACCACC TCTCGAGACT ATCGCAACAT CATTGAAAGA 
CTACAGGATA TAGTGTCTGC ATTGGAGGAG CGGTTGAGGC CTCTGGTTCA GGCTGAGTTG 
TCAGTGTTGG TGGATGTTCT CCATCGCCCA GAGCTGCTGT TTCCAGAGCA CACTGAAGCC 
CACCGCAAGT GTGAGAGTGG AGGCTTCATC TGCAAGTTGA TAAAACACAC TAAGCAGCTT 
CTAGAAGAAA ACGAAGAGAG GCTTTGCATC AAAGTCCTTC AGACACTCCG AGAGATGATG 
ACCAAAGACA GAGGATATGG AGAGAAGGGG GAGGCCCTTC GGCAGCTGCT CGTCAATCGT 
TACTATGGCT TCAGGAGTGG TGGGCGGAGA GAAAGTCTGA CCTCGTTTGG TAACTCCACC 
CTAACTCCTG TCGGGCCAAT CAAGAGCCAG CCAGGGGGGT TGAGCAGGGC TGAGATGTCA 
TTAATGGAGG TGCAGTGTCA TCTAGACCGT GAAGGGGCAT CGGATCTTGT CATTGATCTC 
ATCATGAACA CTACCAGCGA CCGCGTCTTC CATGAAAGCA TTCTGTTGGC CATCGCACTG 
TTGGAGGGCG GAAACACCAT CATACAGCAT TCCTTCTTCA AACGTTTAAC AGAAGACAAA 
AATTCAGAGA AGTTTTTCAG GGTGTTTTAT GACAGAATGA AAGTGGCTCA GGTGGAGATC 
AAAGCCACGG TTACTGTCAA CACTAGTGAC CTCGGCAACA AGCGGCGTGA CGACAACACT 
CCAGACAAAG ATACTCCACA ACGCCGCAGA GGTAAAGACT CCGGTGTGGT GGTGACAGAT 
GATGCTCGTG AGCAGCTGTT GGAGGCTTCA GCTGCGACTA AGAAGGCCTT TGGTTCATAT 
CGTCGAGATG CTGATCCCGA GGAGACGTTT GGCACGGCTG ATGGAGATAA AGGAGGTGGA 
GATAAAGGCA CAGAGCAGGG CGAGATGAGC CCTGTCATCC TCATAATGCA GCCAATCCTG 
CGCTTCCTGC AGCTGCTGTG CGAAAACCAC AATCGAGACC TGCAGAATTT TCTGCGCTGT 
CAGAACAATA AAAACAACTA TAATCTGGTG TGTGAGACGC TGCAGTTCCT GGACTGTATC 
TGTGGCAGCA CAACAGGAGG CCTGGGGCTG TTAGGACTCT ACATCAACCA GCATAACGTA 
GCGCTCATTA ATCAGACTGT AGAGAGCCTC ACTGAATACT GTCAAGGTCC CTGCCATGAC 
AATCAGAATT GCATTGCCAC CCATGAGTCT AATGGCATCG ACATCATTAT TGCACTGATT 
CTGAATGATA TTAATCCACT GGGCAGGAAG AGAATGGATC TGGTTCTAGA ACTAAAGAAT 
AATGCATCTA AGCTGCTGCT GGCCATCATG GAGAGCCGTC ATGACAGTGA GAATGCAGAA 
AGAATTCTGT ACAACATGAG ACCAAAAGAA CTAGTGGAGG TGATGAAAAT GGCCTACCAG 
CAGGGGGAGG CAGAGTTTGA GGATGAGGAA CAGGAGAATG GAGAGGATCA TGCAGCATCG 
CCTCGCAATG TTGGACACAA CATCTATATT CTTGCACATC AGCTGTCGCG TCACAATAAG 
GAATTGCAAA TACTACTGAA ACCAGGTGGA GAGGACCAGG CCCTGGAGTA TTACACAAAA 
CACACCTCAC AGATAGAGAT AGTTCGACAG GATCGCACTA TGGAACAGAT TGTGTTTCCT 
GTACCAAATA TCTGCTCTTT TCTGACAAAT GAGTCCAAGC TGCGTGTGTA CTATGGTACA 
GAGAGAGACG AGCAAGGCAG TAAAATAAAT GACTTCTTCT TGCATGCAGA CGACCTCTTT 
AATGAAATGA GGTGGCAGAA AAAACTAAGA GCTCAGCCAG TGCTGTACTG GTGCAGCAGG 
AACATGTCGT TTTGGAGTAA CGTTTCCTTT AATCTGGCGG TGCTCATTAA TTTGCTGGTG 
GCATTTTTCT ACCCTCTGGA TGGAGTGAGC GAAAGCCAGC TGGAACCTTC TCTGTCTCTG 
TTGCTGTGGG TTTGTTTGTT GGGCTCACTG GGCTTCGTGT TGATGTCCCC GAGGCCAAAT 
GCAGTGCGGG TGCTGGTCAT CTCCACTGTC CTGCAGTTGG GCTTCTCTGT AGGGCTGCAG 
CACATGCTCA CACTCCTGGG AGCTTTCAAT GTTTGTAATA AGATCGTGTT CATGCTGAGC 
TTTGTGGGTA ACCGTGGGTC ATTCACCCGG GGTTATCGGG TGATGGTCAT GGATAGAGAG 
TTCCTGTTTC ATCTGCTGTA CCTTCTCATC TGCATACTGG GCCTTTTTGG ACACGTCTTC 
TTTTACAGTC TTCTGTTGTT TGACCTGGTG AACCGGGAAG AGACTCTTCT GAACGTCATC 
AAGAGTGTGA CTCGAAACGG CCGCTCCATC GTCTTGACCG CAGTGCTGGG CCTCATCCTG 
GTCTACCTCT TTTCTATTGT TGGATATATG TTTTTCAAAG ATGACTTCAT CCTGGAGGTG 
AACCGCATCT CCAACGGCAC TCTGGAGGAG GGGGTGACTT TACCTGCTGG TTTTTGTGAT 
GCGAGAAACG GGTCGTGTCT TTCTGATGAT GTTGACGAGG ATGACGTGGA GCGCGCGTGT 
GATTCTCTAT GGATGTGTAT GATCACTGTA CTCAGTCATG GCCTCAGGAG CGGTGGAGGA 
GTTGGAGATG TCCTGCGCAA ACCGTCTAAA GAGGAGCGTC TGTTCGCCGC ACGGGTGGTG 
TACGATCTCC TGTTCTTCTT CCTGGTGATC ATCATTGTGT TGAATCTGAT CTTCGGTGTG 
ATCATTGACA CATTTGCAGA TCTGAGAAGC GAGAAGCAGA GAAAAGAGGA GATCTTGAAG 
ACCACATGCT TCATCTGTGG TCTGGAGAGA GATAAGTTTG ATAATAAAAC GGTGACGTTT 
GAAGAGCACA TCAAGGAGGA GCACAACCTG TGGCATTACC TCTTCTTCAT AGTGCTGGTG 
CGGGTCAAAG ACTCCACCGA ATACACGGGA CCCGAGAGCT ACGTCGCTCA GATGATCAAG 
GAGCACAATC TGGACTGGTT TCCTCGTATG AGGGCGATGT CTCTGGTCAG CAGTGATGGA 
GAAGGTGAAC AGAATGAACT GCGCAGCTTA CAGGAGAAAC TCGAGTCCAC AATGAGGCTC 
GTCTCTAATC TTACCAATCA GCTCACAGAG CTCAAAGAGC AGATGACTGA GCAAAGGAAA 
CACAAGCAGA GGCTCGGTCT GCTGGGAAAC CCCGCCCACC TCAACATCAA CCCACAGCAG 
CCAGCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itpr1a ( XM_017356666.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212155.1 Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X8, mRNA.

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CloneID	ODa59495
Clone ID Related Accession (Same CDS sequence)	XM_017356666.1 , XM_017356666.2
Accession Version	XM_017356666.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GATGGATTCG 
GAGCATGAAG TGATTCGGGC TCGTGCACGT AACCCTCAGG ATAAGGTGAT GTACACACTG 
GTGTCTGTTC CTGATGGAAA TGACATATCC TCCATCTTTG AGCTTGACCC TACAACTTTG 
AGGGGCGGAG ACTCACTTGT GCCAAGAAAC TCGTATGTGA GGCTCAGGCA TCTCTGCACC 
AACACGTGGG TCCACAGCAC CAACCAGCCA ATAGATAAAG AGGAAGAGAA ACCGGTCATG 
CTCAGAATAG GAACGTCTCC TCTGAAGGAA GATAAAGAGG CGTTTGCTAT AGTACCAGTC 
TCTCCAGCTG AGGTTCGAGA TCTGGATTTT GCTAATGATG CTAGTAAAGT GTTGGCTTCC 
ATTGCTGCCA AACTGGAGAA GGGCACCATT ACCCAGAATG AGAGGAGGTC AGTGACGAAG 
CTGTTGGAGG ATCTGGTGTA TTTTGTGGTG GACATACCCA GCAGTGCTCA AGATGTGCTG 
GAGATCACAG TCAACAAACC CAACAGAGAG CGACAGAAAC TCATGAGAGA GCAGAACATT 
CTTAAACAGA TCTTCAAGCT GCTGCAGGCG CCATTTACAG ACAGCGGGGA CGGCCCTATG 
TTGCGATTAG AAGAGCTCGC AGACCAGCGT CATGCACCCT TTCGACACAT TTGCAGACTC 
TGTTACAGAG TCCTACGTTA CTCACAACAG GACTATCGCA AAAATCAGGA GTACATAGCA 
AAGCAGTTCC GCTTCATGCA GAAGCAGATA GGATATGATG TGCTGGCTGA AGACACAATT 
ACAGCCCTTC TGCACAACAA CAGGAAGCTC CTAGAGAAAC ACATCACAGC AGCTGAAATA 
GATACCTTTG TCAGTCTGGT CCGCAAGAAC AGAGAGCCCA GATTTCTGGA TTACTTGTCA 
GATCTGTGTG TGTCGATGAA TAAATCCATC CCGGTCACTC AAGAGCTCAT CTGTAATGCT 
GTGCTGGACC CAGCCAATGC AGACATTCTC ATCGAAACCA AGCTTGTGTT GTCACGGTTT 
GAGGTGGCAG GAACGGTGTT GGGTGAAGGT GCGGAGGAGG AAGAGGAGGA TGAAGAGGAG 
GTCTGGCTGT TCTGGAAGGA CAGTGGAGGA GAGGTGAAGA GTAAAAGCAT ACGAGAGCTT 
GCACAGGACG CAAAAGATGG ACATACAGAG GACCAGGAAG TCATCAATTA TTACAGATAT 
CAGCTGAATT TGTTTGCTCG GATGTGTCTG GATCGACAGT ATCTTGCGAT TAACAAGATA 
TCAGCACAGC TGGATGTGGA TCTGATTCTG CGCTGCATGT CAGATGAAGA TTTGCCCTTT 
GACCTCAGAG CATCGTTCTG CCGCATGATG CTGCACATGC ATGTAGACCG TGACCCTCAG 
GAGCAGGTCA CCCCGGTCAA ATATGCTCGA CTGTGGTCTG AAATCCCATC ACAAATTTCC 
ATTGATGATT ATGATAATGA TGGGACGTCA AGTGATGAAG TGAAAGAGCG CTTCTCTCAG 
ACGATGGAGT TTGTGGAGAA TTACCTGAGG GATGTTGTCT GCCAGAGTTT CCCGTTCTCT 
GATAAGGAGA AAAACAAACT CACTTTTGAG GTGGTGAACC TGGCAAGAAA CCTGATCTAT 
TTTGGTTTCT ATAACTTCAG TGATCTGCTA AGGTTAACAA AAATCCTATT GGCAATCCTG 
GACTGTGTCC ACATAAGCAC TCCCTTCCAC ATCAAGCTGG ATAGAGAACC CGGTAAAGGA 
AGTAACGTGA TGCGCTCCAT CCATGGGGTG GGAGAGCTGA TGACACAGGT GGTGTTGAGG 
GGTGGGGGAT TTCTGCCAGC CTCCAGCCAC AATCCTCCTG ACAGAGACGA AGTGAAAGCA 
CAAAGTGAAC CTCAGAAACA GGACATACTG GTGATGGACA CTAAACTCAA GATCATCGAG 
ATCCTGCAGT TTATTCTGAA TGTGCGGTTG GACTACAGGA TCTCCTGCCT CCTCTGTATC 
TTTAAGACAG AGTTTGATGA AAGTAACTCT CAAAGTGAAC CATCTTTAAG CCCAGAATCT 
CCTGCCAGCG TTCAAGGTGC GCTAGACTTT GAGCACATTG AAGAGCAGGC GGAGGGTATT 
TTTGGAGGAA GTGAAGAGAA CACCCCGCTG GATCTTGATG ATCATGGTGG ACGGACATTT 
CTGAGGGTCC TGCTTCACCT GACCATGCAT GACTATCCTC CGCTGGTATC TCGAGCTCTT 
CACCTGCTCT TCAGACACTT TAGCCAACGC CAGGAGGTCC TACAGGCCTT CAAACAGGTT 
CAGCTCCTGG TGACCAGTCA GGATGTGGAA AACTATAAAC AGATCAAGTC AGATTTGGAC 
CAGCTGAGGT CAATAGTAGA GAAATCAGAG TTGTGGGTGT ATAAGAGACA AGGCTCAGAC 
TCAGGACTGG ATGCTGGAGA AGTCACCCCT GAAGCCCACC ACAAGGGTGT CATCAACTCG 
AGTAGATCAA ACAAACCCAA AGTGGAAAGT ACCAGCAGCT CGAACTACAG AGTCGTGAAA 
GAGATCCTGC TGAGGCTGAG CAAACTGTGT GTTTTGGAGG GCATCTCTGG CAAGAAGAAT 
AAGAAACAGC AGCAGCGGCT CCTCAGGAAC ATGGGAGCTC ACAGTGTGGT CCTCGAACTG 
TTGCAAATCC CTTATGAGAA GGGGGAGGAT GTCCAGATGC AGGAGATCAT GACCTTAGCA 
CATCAGTTCC TTCAAAACTT CTGTGCTGGA AATCAGCAGA ACCAGGCACT TCTACACAAA 
CACATCAACC TGTTCCTCAA CCCTGGGATC CTGGAGGCGG TGACCATGCA GCATATCTTC 
ATGAATAATT TCCAGTTGTG TAGTGAGATT AATGAGCGGG TCGTGCAGCA TTTTGTGCAC 
TGCATCGAGA CACACGGCCG CAGCGTGCAC TACCTCAAGT TCCTGCAGAC CATTGTCAAG 
GCAGAGAACA AGTTCATCAA GAAATGCCAA GACATCGTCA TGGCTGAGCT GGTGACTGCA 
GGTGAGGATG TGCTGGTGTT CTATAATGAC CGGGCGTCTT TTCAGTCATT GGTTCAGATG 
ATGCGTCTTG AGCGGGAACG ACTTGATGAG AGCAGTGCAC TCAGGTATCA CATTCACCTG 
GTGGAGTTGC TGGCTGTGTG CACTGAAGGC AAGAACGTCT ACACTGAGAT CAAATGCAAC 
TCGCTGCTCC CTCTCGATGA TATTGTACGT GTGGTCACAC ATGAGGACTG CATCCCTGAG 
GTGAAGATGG CCTACGTGAA CTTCCTGAAT CACTGTTATG TTGATACAGA GGTTGAGATG 
AAGGAGATCT ACACCAGCAA TCACATGTGG AAACTTTTTG ATGATTTTCT GGTGGACGTC 
TGCAGAGTGT GCAATAACAC AAGTGACCGT AAACACGCTG ACACAGTTCT GGAGAGATAC 
GTTACAGAGA CCATAATGAG CATCGTCACC ACCTTTTTCA GCTCACCATT CTCAGACCAG 
AGCACAAGTT TGCAGACCCG GCAGCCGGTG TTTGTGCAGC TGCTGCAGGG AGTGTTTCGT 
GTATATCACT GCAACTGGCT TCTCCCAGGT CAGAAGGGGA ATGTGGAAGC CTGCATCAAA 
GTGCTGAGTG ACGTGGCCAA AGGCAGAGCC ATAGCCATTC CTGTGGATCT GGACTGTCAG 
GTGAATAATC TGTTCATGAA GTCCAACAAC ATTGTGCAGA AAACAGCCCT CAGCTGGAGA 
CTCTCTGTCC GTAACGCGAC TCGGAGAGAC TCTGTTCTGA CCACCTCTCG AGACTATCGC 
AACATCATTG AAAGACTACA GGATATAGTG TCTGCATTGG AGGAGCGGTT GAGGCCTCTG 
GTTCAGGCTG AGTTGTCAGT GTTGGTGGAT GTTCTCCATC GCCCAGAGCT GCTGTTTCCA 
GAGCACACTG AAGCCCACCG CAAGTGTGAG AGTGGAGGCT TCATCTGCAA GTTGATAAAA 
CACACTAAGC AGCTTCTAGA AGAAAACGAA GAGAGGCTTT GCATCAAAGT CCTTCAGACA 
CTCCGAGAGA TGATGACCAA AGACAGAGGA TATGGAGAGA AGGGGGAGGC CCTTCGGCAG 
CTGCTCGTCA ATCGTTACTA TGGCTTCAGG AGTGGTGGGC GGAGAGAAAG TCTGACCTCG 
TTTGGTAACT CCACCCTAAC TCCTGTCGGG CCAATCAAGA GCCAGCCAGG GGGGTTGAGC 
AGGGCTGAGA TGTCATTAAT GGAGGTGCAG TGTCATCTAG ACCGTGAAGG GGCATCGGAT 
CTTGTCATTG ATCTCATCAT GAACACTACC AGCGACCGCG TCTTCCATGA AAGCATTCTG 
TTGGCCATCG CACTGTTGGA GGGCGGAAAC ACCATCATAC AGCATTCCTT CTTCAAACGT 
TTAACAGAAG ACAAAAATTC AGAGAAGTTT TTCAGGGTGT TTTATGACAG AATGAAAGTG 
GCTCAGGTGG AGATCAAAGC CACGGTTACT GTCAACACTA GTGACCTCGG CAACAAGCGG 
CGTGACGACA ACACTCCAGA CAAAGATACT CCACAACGCC GCAGAGGTAA AGACTCCGGT 
GTGGTGGTGA CAGATGATGC TCGTGAGCAG CTGTTGGAGG CTTCAGCTGC GACTAAGAAG 
GCCTTTGGTT CATATCGTCG AGATGCTGAT CCCGAGGAGA CGTTTGGCAC GGCTGATGGA 
GATAAAGGAG GTGGAGATAA AGGCACAGAG CAGGGCGAGA TGAGCCCTGT CATCCTCATA 
ATGCAGCCAA TCCTGCGCTT CCTGCAGCTG CTGTGCGAAA ACCACAATCG AGACCTGCAG 
AATTTTCTGC GCTGTCAGAA CAATAAAAAC AACTATAATC TGGTGTGTGA GACGCTGCAG 
TTCCTGGACT GTATCTGTGG CAGCACAACA GGAGGCCTGG GGCTGTTAGG ACTCTACATC 
AACCAGCATA ACGTAGCGCT CATTAATCAG ACTGTAGAGA GCCTCACTGA ATACTGTCAA 
GGTCCCTGCC ATGACAATCA GAATTGCATT GCCACCCATG AGTCTAATGG CATCGACATC 
ATTATTGCAC TGATTCTGAA TGATATTAAT CCACTGGGCA GGAAGAGAAT GGATCTGGTT 
CTAGAACTAA AGAATAATGC ATCTAAGCTG CTGCTGGCCA TCATGGAGAG CCGTCATGAC 
AGTGAGAATG CAGAAAGAAT TCTGTACAAC ATGAGACCAA AAGAACTAGT GGAGGTGATG 
AAAATGGCCT ACCAGCAGGG GGAGGCAGAG TTTGAGGATG AGGAACAGGA GAATGGAGAG 
GATCATGCAG CATCGCCTCG CAATGTTGGA CACAACATCT ATATTCTTGC ACATCAGCTG 
TCGCGTCACA ATAAGGAATT GCAAATACTA CTGAAACCAG GTGGAGAGGA CCAGGCCCTG 
GAGTATTACA CAAAACACAC CTCACAGATA GAGATAGTTC GACAGGATCG CACTATGGAA 
CAGATTGTGT TTCCTGTACC AAATATCTGC TCTTTTCTGA CAAATGAGTC CAAGCTGCGT 
GTGTACTATG GTACAGAGAG AGACGAGCAA GGCAGTAAAA TAAATGACTT CTTCTTGCAT 
GCAGACGACC TCTTTAATGA AATGAGGTGG CAGAAAAAAC TAAGAGCTCA GCCAGTGCTG 
TACTGGTGCA GCAGGAACAT GTCGTTTTGG AGTAACGTTT CCTTTAATCT GGCGGTGCTC 
ATTAATTTGC TGGTGGCATT TTTCTACCCT CTGGATGGAG TGAGCGAAAG CCAGCTGGAA 
CCTTCTCTGT CTCTGTTGCT GTGGGTTTGT TTGTTGGGCT CACTGGGCTT CGTGTTGATG 
TCCCCGAGGC CAAATGCAGT GCGGGTGCTG GTCATCTCCA CTGTCCTGCA GTTGGGCTTC 
TCTGTAGGGC TGCAGCACAT GCTCACACTC CTGGGAGCTT TCAATGTTTG TAATAAGATC 
GTGTTCATGC TGAGCTTTGT GGGTAACCGT GGGTCATTCA CCCGGGGTTA TCGGGTGATG 
GTCATGGATA GAGAGTTCCT GTTTCATCTG CTGTACCTTC TCATCTGCAT ACTGGGCCTT 
TTTGGACACG TCTTCTTTTA CAGTCTTCTG TTGTTTGACC TGGTGAACCG GGAAGAGACT 
CTTCTGAACG TCATCAAGAG TGTGACTCGA AACGGCCGCT CCATCGTCTT GACCGCAGTG 
CTGGGCCTCA TCCTGGTCTA CCTCTTTTCT ATTGTTGGAT ATATGTTTTT CAAAGATGAC 
TTCATCCTGG AGGTGAACCG CATCTCCAAC GGCACTCTGG AGGAGGGGGT GACTTTACCT 
GCTGGTTTTT GTGATGCGAG AAACGGGTCG TGTCTTTCTG ATGATGTTGA CGAGGATGAC 
GTGGAGCGCG CGTGTGATTC TCTATGGATG TGTATGATCA CTGTACTCAG TCATGGCCTC 
AGGAGCGGTG GAGGAGTTGG AGATGTCCTG CGCAAACCGT CTAAAGAGGA GCGTCTGTTC 
GCCGCACGGG TGGTGTACGA TCTCCTGTTC TTCTTCCTGG TGATCATCAT TGTGTTGAAT 
CTGATCTTCG GTGTGATCAT TGACACATTT GCAGATCTGA GAAGCGAGAA GCAGAGAAAA 
GAGGAGATCT TGAAGACCAC ATGCTTCATC TGTGGTCTGG AGAGAGATAA GTTTGATAAT 
AAAACGGTGA CGTTTGAAGA GCACATCAAG GAGGAGCACA ACCTGTGGCA TTACCTCTTC 
TTCATAGTGC TGGTGCGGGT CAAAGACTCC ACCGAATACA CGGGACCCGA GAGCTACGTC 
GCTCAGATGA TCAAGGAGCA CAATCTGGAC TGGTTTCCTC GTATGAGGGC GATGTCTCTG 
GTCAGCAGTG ATGGAGAAGG TGAACAGAAT GAACTGCGCA GCTTACAGGA GAAACTCGAG 
TCCACAATGA GGCTCGTCTC TAATCTTACC AATCAGCTCA CAGAGCTCAA AGAGCAGATG 
ACTGAGCAAA GGAAACACAA GCAGAGGCTC GGTCTGCTGG GAAACCCCGC CCACCTCAAC 
ATCAACCCAC AGCAGCCAGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212155.1
CDS	148..8151
Translation	MVDKLSSFLHIGDVCSLYAEGSTSGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGTSTTDTVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDAAGNEGSWFYIQPFYKLRSIGDNVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCTTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDEHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEMDSEHEVIRARARNPQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSPLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAAKLEKGTITQNERRSVTKLLEDLVYFVVDIPSSAQDVLEITVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRYSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDPANADILIETKLVLSRFEVAGTVLGEGAEEEEEDEEEVWLFWKDSGGEVKSKSIRELAQDAKDGHTEDQEVINYYRYQLNLFARMCLDRQYLAINKISAQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSQISIDDYDNDGTSSDEVKERFSQTMEFVENYLRDVVCQSFPFSDKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHISTPFHIKLDREPGKGSNVMRSIHGVGELMTQVVLRGGGFLPASSHNPPDRDEVKAQSEPQKQDILVMDTKLKIIEILQFILNVRLDYRISCLLCIFKTEFDESNSQSEPSLSPESPASVQGALDFEHIEEQAEGIFGGSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALHLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSDSGLDAGEVTPEAHHKGVINSSRSNKPKVESTSSSNYRVVKEILLRLSKLCVLEGISGKKNKKQQQRLLRNMGAHSVVLELLQIPYEKGEDVQMQEIMTLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAVTMQHIFMNNFQLCSEINERVVQHFVHCIETHGRSVHYLKFLQTIVKAENKFIKKCQDIVMAELVTAGEDVLVFYNDRASFQSLVQMMRLERERLDESSALRYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKMAYVNFLNHCYVDTEVEMKEIYTSNHMWKLFDDFLVDVCRVCNNTSDRKHADTVLERYVTETIMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQGVFRVYHCNWLLPGQKGNVEACIKVLSDVAKGRAIAIPVDLDCQVNNLFMKSNNIVQKTALSWRLSVRNATRRDSVLTTSRDYRNIIERLQDIVSALEERLRPLVQAELSVLVDVLHRPELLFPEHTEAHRKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKGEALRQLLVNRYYGFRSGGRRESLTSFGNSTLTPVGPIKSQPGGLSRAEMSLMEVQCHLDREGASDLVIDLIMNTTSDRVFHESILLAIALLEGGNTIIQHSFFKRLTEDKNSEKFFRVFYDRMKVAQVEIKATVTVNTSDLGNKRRDDNTPDKDTPQRRRGKDSGVVVTDDAREQLLEASAATKKAFGSYRRDADPEETFGTADGDKGGGDKGTEQGEMSPVILIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINQHNVALINQTVESLTEYCQGPCHDNQNCIATHESNGIDIIIALILNDINPLGRKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVMKMAYQQGEAEFEDEEQENGEDHAASPRNVGHNIYILAHQLSRHNKELQILLKPGGEDQALEYYTKHTSQIEIVRQDRTMEQIVFPVPNICSFLTNESKLRVYYGTERDEQGSKINDFFLHADDLFNEMRWQKKLRAQPVLYWCSRNMSFWSNVSFNLAVLINLLVAFFYPLDGVSESQLEPSLSLLLWVCLLGSLGFVLMSPRPNAVRVLVISTVLQLGFSVGLQHMLTLLGAFNVCNKIVFMLSFVGNRGSFTRGYRVMVMDREFLFHLLYLLICILGLFGHVFFYSLLLFDLVNREETLLNVIKSVTRNGRSIVLTAVLGLILVYLFSIVGYMFFKDDFILEVNRISNGTLEEGVTLPAGFCDARNGSCLSDDVDEDDVERACDSLWMCMITVLSHGLRSGGGVGDVLRKPSKEERLFAARVVYDLLFFFLVIIIVLNLIFGVIIDTFADLRSEKQRKEEILKTTCFICGLERDKFDNKTVTFEEHIKEEHNLWHYLFFIVLVRVKDSTEYTGPESYVAQMIKEHNLDWFPRMRAMSLVSSDGEGEQNELRSLQEKLESTMRLVSNLTNQLTELKEQMTEQRKHKQRLGLLGNPAHLNINPQQPA

Target ORF information:

RefSeq Version	XM_017356666.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356666.1

ORF Insert Sequence:

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GATGGATTCG 
GAGCATGAAG TGATTCGGGC TCGTGCACGT AACCCTCAGG ATAAGGTGAT GTACACACTG 
GTGTCTGTTC CTGATGGAAA TGACATATCC TCCATCTTTG AGCTTGACCC TACAACTTTG 
AGGGGCGGAG ACTCACTTGT GCCAAGAAAC TCGTATGTGA GGCTCAGGCA TCTCTGCACC 
AACACGTGGG TCCACAGCAC CAACCAGCCA ATAGATAAAG AGGAAGAGAA ACCGGTCATG 
CTCAGAATAG GAACGTCTCC TCTGAAGGAA GATAAAGAGG CGTTTGCTAT AGTACCAGTC 
TCTCCAGCTG AGGTTCGAGA TCTGGATTTT GCTAATGATG CTAGTAAAGT GTTGGCTTCC 
ATTGCTGCCA AACTGGAGAA GGGCACCATT ACCCAGAATG AGAGGAGGTC AGTGACGAAG 
CTGTTGGAGG ATCTGGTGTA TTTTGTGGTG GACATACCCA GCAGTGCTCA AGATGTGCTG 
GAGATCACAG TCAACAAACC CAACAGAGAG CGACAGAAAC TCATGAGAGA GCAGAACATT 
CTTAAACAGA TCTTCAAGCT GCTGCAGGCG CCATTTACAG ACAGCGGGGA CGGCCCTATG 
TTGCGATTAG AAGAGCTCGC AGACCAGCGT CATGCACCCT TTCGACACAT TTGCAGACTC 
TGTTACAGAG TCCTACGTTA CTCACAACAG GACTATCGCA AAAATCAGGA GTACATAGCA 
AAGCAGTTCC GCTTCATGCA GAAGCAGATA GGATATGATG TGCTGGCTGA AGACACAATT 
ACAGCCCTTC TGCACAACAA CAGGAAGCTC CTAGAGAAAC ACATCACAGC AGCTGAAATA 
GATACCTTTG TCAGTCTGGT CCGCAAGAAC AGAGAGCCCA GATTTCTGGA TTACTTGTCA 
GATCTGTGTG TGTCGATGAA TAAATCCATC CCGGTCACTC AAGAGCTCAT CTGTAATGCT 
GTGCTGGACC CAGCCAATGC AGACATTCTC ATCGAAACCA AGCTTGTGTT GTCACGGTTT 
GAGGTGGCAG GAACGGTGTT GGGTGAAGGT GCGGAGGAGG AAGAGGAGGA TGAAGAGGAG 
GTCTGGCTGT TCTGGAAGGA CAGTGGAGGA GAGGTGAAGA GTAAAAGCAT ACGAGAGCTT 
GCACAGGACG CAAAAGATGG ACATACAGAG GACCAGGAAG TCATCAATTA TTACAGATAT 
CAGCTGAATT TGTTTGCTCG GATGTGTCTG GATCGACAGT ATCTTGCGAT TAACAAGATA 
TCAGCACAGC TGGATGTGGA TCTGATTCTG CGCTGCATGT CAGATGAAGA TTTGCCCTTT 
GACCTCAGAG CATCGTTCTG CCGCATGATG CTGCACATGC ATGTAGACCG TGACCCTCAG 
GAGCAGGTCA CCCCGGTCAA ATATGCTCGA CTGTGGTCTG AAATCCCATC ACAAATTTCC 
ATTGATGATT ATGATAATGA TGGGACGTCA AGTGATGAAG TGAAAGAGCG CTTCTCTCAG 
ACGATGGAGT TTGTGGAGAA TTACCTGAGG GATGTTGTCT GCCAGAGTTT CCCGTTCTCT 
GATAAGGAGA AAAACAAACT CACTTTTGAG GTGGTGAACC TGGCAAGAAA CCTGATCTAT 
TTTGGTTTCT ATAACTTCAG TGATCTGCTA AGGTTAACAA AAATCCTATT GGCAATCCTG 
GACTGTGTCC ACATAAGCAC TCCCTTCCAC ATCAAGCTGG ATAGAGAACC CGGTAAAGGA 
AGTAACGTGA TGCGCTCCAT CCATGGGGTG GGAGAGCTGA TGACACAGGT GGTGTTGAGG 
GGTGGGGGAT TTCTGCCAGC CTCCAGCCAC AATCCTCCTG ACAGAGACGA AGTGAAAGCA 
CAAAGTGAAC CTCAGAAACA GGACATACTG GTGATGGACA CTAAACTCAA GATCATCGAG 
ATCCTGCAGT TTATTCTGAA TGTGCGGTTG GACTACAGGA TCTCCTGCCT CCTCTGTATC 
TTTAAGACAG AGTTTGATGA AAGTAACTCT CAAAGTGAAC CATCTTTAAG CCCAGAATCT 
CCTGCCAGCG TTCAAGGTGC GCTAGACTTT GAGCACATTG AAGAGCAGGC GGAGGGTATT 
TTTGGAGGAA GTGAAGAGAA CACCCCGCTG GATCTTGATG ATCATGGTGG ACGGACATTT 
CTGAGGGTCC TGCTTCACCT GACCATGCAT GACTATCCTC CGCTGGTATC TCGAGCTCTT 
CACCTGCTCT TCAGACACTT TAGCCAACGC CAGGAGGTCC TACAGGCCTT CAAACAGGTT 
CAGCTCCTGG TGACCAGTCA GGATGTGGAA AACTATAAAC AGATCAAGTC AGATTTGGAC 
CAGCTGAGGT CAATAGTAGA GAAATCAGAG TTGTGGGTGT ATAAGAGACA AGGCTCAGAC 
TCAGGACTGG ATGCTGGAGA AGTCACCCCT GAAGCCCACC ACAAGGGTGT CATCAACTCG 
AGTAGATCAA ACAAACCCAA AGTGGAAAGT ACCAGCAGCT CGAACTACAG AGTCGTGAAA 
GAGATCCTGC TGAGGCTGAG CAAACTGTGT GTTTTGGAGG GCATCTCTGG CAAGAAGAAT 
AAGAAACAGC AGCAGCGGCT CCTCAGGAAC ATGGGAGCTC ACAGTGTGGT CCTCGAACTG 
TTGCAAATCC CTTATGAGAA GGGGGAGGAT GTCCAGATGC AGGAGATCAT GACCTTAGCA 
CATCAGTTCC TTCAAAACTT CTGTGCTGGA AATCAGCAGA ACCAGGCACT TCTACACAAA 
CACATCAACC TGTTCCTCAA CCCTGGGATC CTGGAGGCGG TGACCATGCA GCATATCTTC 
ATGAATAATT TCCAGTTGTG TAGTGAGATT AATGAGCGGG TCGTGCAGCA TTTTGTGCAC 
TGCATCGAGA CACACGGCCG CAGCGTGCAC TACCTCAAGT TCCTGCAGAC CATTGTCAAG 
GCAGAGAACA AGTTCATCAA GAAATGCCAA GACATCGTCA TGGCTGAGCT GGTGACTGCA 
GGTGAGGATG TGCTGGTGTT CTATAATGAC CGGGCGTCTT TTCAGTCATT GGTTCAGATG 
ATGCGTCTTG AGCGGGAACG ACTTGATGAG AGCAGTGCAC TCAGGTATCA CATTCACCTG 
GTGGAGTTGC TGGCTGTGTG CACTGAAGGC AAGAACGTCT ACACTGAGAT CAAATGCAAC 
TCGCTGCTCC CTCTCGATGA TATTGTACGT GTGGTCACAC ATGAGGACTG CATCCCTGAG 
GTGAAGATGG CCTACGTGAA CTTCCTGAAT CACTGTTATG TTGATACAGA GGTTGAGATG 
AAGGAGATCT ACACCAGCAA TCACATGTGG AAACTTTTTG ATGATTTTCT GGTGGACGTC 
TGCAGAGTGT GCAATAACAC AAGTGACCGT AAACACGCTG ACACAGTTCT GGAGAGATAC 
GTTACAGAGA CCATAATGAG CATCGTCACC ACCTTTTTCA GCTCACCATT CTCAGACCAG 
AGCACAAGTT TGCAGACCCG GCAGCCGGTG TTTGTGCAGC TGCTGCAGGG AGTGTTTCGT 
GTATATCACT GCAACTGGCT TCTCCCAGGT CAGAAGGGGA ATGTGGAAGC CTGCATCAAA 
GTGCTGAGTG ACGTGGCCAA AGGCAGAGCC ATAGCCATTC CTGTGGATCT GGACTGTCAG 
GTGAATAATC TGTTCATGAA GTCCAACAAC ATTGTGCAGA AAACAGCCCT CAGCTGGAGA 
CTCTCTGTCC GTAACGCGAC TCGGAGAGAC TCTGTTCTGA CCACCTCTCG AGACTATCGC 
AACATCATTG AAAGACTACA GGATATAGTG TCTGCATTGG AGGAGCGGTT GAGGCCTCTG 
GTTCAGGCTG AGTTGTCAGT GTTGGTGGAT GTTCTCCATC GCCCAGAGCT GCTGTTTCCA 
GAGCACACTG AAGCCCACCG CAAGTGTGAG AGTGGAGGCT TCATCTGCAA GTTGATAAAA 
CACACTAAGC AGCTTCTAGA AGAAAACGAA GAGAGGCTTT GCATCAAAGT CCTTCAGACA 
CTCCGAGAGA TGATGACCAA AGACAGAGGA TATGGAGAGA AGGGGGAGGC CCTTCGGCAG 
CTGCTCGTCA ATCGTTACTA TGGCTTCAGG AGTGGTGGGC GGAGAGAAAG TCTGACCTCG 
TTTGGTAACT CCACCCTAAC TCCTGTCGGG CCAATCAAGA GCCAGCCAGG GGGGTTGAGC 
AGGGCTGAGA TGTCATTAAT GGAGGTGCAG TGTCATCTAG ACCGTGAAGG GGCATCGGAT 
CTTGTCATTG ATCTCATCAT GAACACTACC AGCGACCGCG TCTTCCATGA AAGCATTCTG 
TTGGCCATCG CACTGTTGGA GGGCGGAAAC ACCATCATAC AGCATTCCTT CTTCAAACGT 
TTAACAGAAG ACAAAAATTC AGAGAAGTTT TTCAGGGTGT TTTATGACAG AATGAAAGTG 
GCTCAGGTGG AGATCAAAGC CACGGTTACT GTCAACACTA GTGACCTCGG CAACAAGCGG 
CGTGACGACA ACACTCCAGA CAAAGATACT CCACAACGCC GCAGAGGTAA AGACTCCGGT 
GTGGTGGTGA CAGATGATGC TCGTGAGCAG CTGTTGGAGG CTTCAGCTGC GACTAAGAAG 
GCCTTTGGTT CATATCGTCG AGATGCTGAT CCCGAGGAGA CGTTTGGCAC GGCTGATGGA 
GATAAAGGAG GTGGAGATAA AGGCACAGAG CAGGGCGAGA TGAGCCCTGT CATCCTCATA 
ATGCAGCCAA TCCTGCGCTT CCTGCAGCTG CTGTGCGAAA ACCACAATCG AGACCTGCAG 
AATTTTCTGC GCTGTCAGAA CAATAAAAAC AACTATAATC TGGTGTGTGA GACGCTGCAG 
TTCCTGGACT GTATCTGTGG CAGCACAACA GGAGGCCTGG GGCTGTTAGG ACTCTACATC 
AACCAGCATA ACGTAGCGCT CATTAATCAG ACTGTAGAGA GCCTCACTGA ATACTGTCAA 
GGTCCCTGCC ATGACAATCA GAATTGCATT GCCACCCATG AGTCTAATGG CATCGACATC 
ATTATTGCAC TGATTCTGAA TGATATTAAT CCACTGGGCA GGAAGAGAAT GGATCTGGTT 
CTAGAACTAA AGAATAATGC ATCTAAGCTG CTGCTGGCCA TCATGGAGAG CCGTCATGAC 
AGTGAGAATG CAGAAAGAAT TCTGTACAAC ATGAGACCAA AAGAACTAGT GGAGGTGATG 
AAAATGGCCT ACCAGCAGGG GGAGGCAGAG TTTGAGGATG AGGAACAGGA GAATGGAGAG 
GATCATGCAG CATCGCCTCG CAATGTTGGA CACAACATCT ATATTCTTGC ACATCAGCTG 
TCGCGTCACA ATAAGGAATT GCAAATACTA CTGAAACCAG GTGGAGAGGA CCAGGCCCTG 
GAGTATTACA CAAAACACAC CTCACAGATA GAGATAGTTC GACAGGATCG CACTATGGAA 
CAGATTGTGT TTCCTGTACC AAATATCTGC TCTTTTCTGA CAAATGAGTC CAAGCTGCGT 
GTGTACTATG GTACAGAGAG AGACGAGCAA GGCAGTAAAA TAAATGACTT CTTCTTGCAT 
GCAGACGACC TCTTTAATGA AATGAGGTGG CAGAAAAAAC TAAGAGCTCA GCCAGTGCTG 
TACTGGTGCA GCAGGAACAT GTCGTTTTGG AGTAACGTTT CCTTTAATCT GGCGGTGCTC 
ATTAATTTGC TGGTGGCATT TTTCTACCCT CTGGATGGAG TGAGCGAAAG CCAGCTGGAA 
CCTTCTCTGT CTCTGTTGCT GTGGGTTTGT TTGTTGGGCT CACTGGGCTT CGTGTTGATG 
TCCCCGAGGC CAAATGCAGT GCGGGTGCTG GTCATCTCCA CTGTCCTGCA GTTGGGCTTC 
TCTGTAGGGC TGCAGCACAT GCTCACACTC CTGGGAGCTT TCAATGTTTG TAATAAGATC 
GTGTTCATGC TGAGCTTTGT GGGTAACCGT GGGTCATTCA CCCGGGGTTA TCGGGTGATG 
GTCATGGATA GAGAGTTCCT GTTTCATCTG CTGTACCTTC TCATCTGCAT ACTGGGCCTT 
TTTGGACACG TCTTCTTTTA CAGTCTTCTG TTGTTTGACC TGGTGAACCG GGAAGAGACT 
CTTCTGAACG TCATCAAGAG TGTGACTCGA AACGGCCGCT CCATCGTCTT GACCGCAGTG 
CTGGGCCTCA TCCTGGTCTA CCTCTTTTCT ATTGTTGGAT ATATGTTTTT CAAAGATGAC 
TTCATCCTGG AGGTGAACCG CATCTCCAAC GGCACTCTGG AGGAGGGGGT GACTTTACCT 
GCTGGTTTTT GTGATGCGAG AAACGGGTCG TGTCTTTCTG ATGATGTTGA CGAGGATGAC 
GTGGAGCGCG CGTGTGATTC TCTATGGATG TGTATGATCA CTGTACTCAG TCATGGCCTC 
AGGAGCGGTG GAGGAGTTGG AGATGTCCTG CGCAAACCGT CTAAAGAGGA GCGTCTGTTC 
GCCGCACGGG TGGTGTACGA TCTCCTGTTC TTCTTCCTGG TGATCATCAT TGTGTTGAAT 
CTGATCTTCG GTGTGATCAT TGACACATTT GCAGATCTGA GAAGCGAGAA GCAGAGAAAA 
GAGGAGATCT TGAAGACCAC ATGCTTCATC TGTGGTCTGG AGAGAGATAA GTTTGATAAT 
AAAACGGTGA CGTTTGAAGA GCACATCAAG GAGGAGCACA ACCTGTGGCA TTACCTCTTC 
TTCATAGTGC TGGTGCGGGT CAAAGACTCC ACCGAATACA CGGGACCCGA GAGCTACGTC 
GCTCAGATGA TCAAGGAGCA CAATCTGGAC TGGTTTCCTC GTATGAGGGC GATGTCTCTG 
GTCAGCAGTG ATGGAGAAGG TGAACAGAAT GAACTGCGCA GCTTACAGGA GAAACTCGAG 
TCCACAATGA GGCTCGTCTC TAATCTTACC AATCAGCTCA CAGAGCTCAA AGAGCAGATG 
ACTGAGCAAA GGAAACACAA GCAGAGGCTC GGTCTGCTGG GAAACCCCGC CCACCTCAAC 
ATCAACCCAC AGCAGCCAGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

itpr1a ( XM_017356666.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017212155.1 Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X8, mRNA.

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CloneID	ODa59495
Clone ID Related Accession (Same CDS sequence)	XM_017356666.1 , XM_017356666.2
Accession Version	XM_017356666.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	inositol 1,4,5-trisphosphate receptor type 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356666.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GATGGATTCG 
GAGCATGAAG TGATTCGGGC TCGTGCACGT AACCCTCAGG ATAAGGTGAT GTACACACTG 
GTGTCTGTTC CTGATGGAAA TGACATATCC TCCATCTTTG AGCTTGACCC TACAACTTTG 
AGGGGCGGAG ACTCACTTGT GCCAAGAAAC TCGTATGTGA GGCTCAGGCA TCTCTGCACC 
AACACGTGGG TCCACAGCAC CAACCAGCCA ATAGATAAAG AGGAAGAGAA ACCGGTCATG 
CTCAGAATAG GAACGTCTCC TCTGAAGGAA GATAAAGAGG CGTTTGCTAT AGTACCAGTC 
TCTCCAGCTG AGGTTCGAGA TCTGGATTTT GCTAATGATG CTAGTAAAGT GTTGGCTTCC 
ATTGCTGCCA AACTGGAGAA GGGCACCATT ACCCAGAATG AGAGGAGGTC AGTGACGAAG 
CTGTTGGAGG ATCTGGTGTA TTTTGTGGTG GACATACCCA GCAGTGCTCA AGATGTGCTG 
GAGATCACAG TCAACAAACC CAACAGAGAG CGACAGAAAC TCATGAGAGA GCAGAACATT 
CTTAAACAGA TCTTCAAGCT GCTGCAGGCG CCATTTACAG ACAGCGGGGA CGGCCCTATG 
TTGCGATTAG AAGAGCTCGC AGACCAGCGT CATGCACCCT TTCGACACAT TTGCAGACTC 
TGTTACAGAG TCCTACGTTA CTCACAACAG GACTATCGCA AAAATCAGGA GTACATAGCA 
AAGCAGTTCC GCTTCATGCA GAAGCAGATA GGATATGATG TGCTGGCTGA AGACACAATT 
ACAGCCCTTC TGCACAACAA CAGGAAGCTC CTAGAGAAAC ACATCACAGC AGCTGAAATA 
GATACCTTTG TCAGTCTGGT CCGCAAGAAC AGAGAGCCCA GATTTCTGGA TTACTTGTCA 
GATCTGTGTG TGTCGATGAA TAAATCCATC CCGGTCACTC AAGAGCTCAT CTGTAATGCT 
GTGCTGGACC CAGCCAATGC AGACATTCTC ATCGAAACCA AGCTTGTGTT GTCACGGTTT 
GAGGTGGCAG GAACGGTGTT GGGTGAAGGT GCGGAGGAGG AAGAGGAGGA TGAAGAGGAG 
GTCTGGCTGT TCTGGAAGGA CAGTGGAGGA GAGGTGAAGA GTAAAAGCAT ACGAGAGCTT 
GCACAGGACG CAAAAGATGG ACATACAGAG GACCAGGAAG TCATCAATTA TTACAGATAT 
CAGCTGAATT TGTTTGCTCG GATGTGTCTG GATCGACAGT ATCTTGCGAT TAACAAGATA 
TCAGCACAGC TGGATGTGGA TCTGATTCTG CGCTGCATGT CAGATGAAGA TTTGCCCTTT 
GACCTCAGAG CATCGTTCTG CCGCATGATG CTGCACATGC ATGTAGACCG TGACCCTCAG 
GAGCAGGTCA CCCCGGTCAA ATATGCTCGA CTGTGGTCTG AAATCCCATC ACAAATTTCC 
ATTGATGATT ATGATAATGA TGGGACGTCA AGTGATGAAG TGAAAGAGCG CTTCTCTCAG 
ACGATGGAGT TTGTGGAGAA TTACCTGAGG GATGTTGTCT GCCAGAGTTT CCCGTTCTCT 
GATAAGGAGA AAAACAAACT CACTTTTGAG GTGGTGAACC TGGCAAGAAA CCTGATCTAT 
TTTGGTTTCT ATAACTTCAG TGATCTGCTA AGGTTAACAA AAATCCTATT GGCAATCCTG 
GACTGTGTCC ACATAAGCAC TCCCTTCCAC ATCAAGCTGG ATAGAGAACC CGGTAAAGGA 
AGTAACGTGA TGCGCTCCAT CCATGGGGTG GGAGAGCTGA TGACACAGGT GGTGTTGAGG 
GGTGGGGGAT TTCTGCCAGC CTCCAGCCAC AATCCTCCTG ACAGAGACGA AGTGAAAGCA 
CAAAGTGAAC CTCAGAAACA GGACATACTG GTGATGGACA CTAAACTCAA GATCATCGAG 
ATCCTGCAGT TTATTCTGAA TGTGCGGTTG GACTACAGGA TCTCCTGCCT CCTCTGTATC 
TTTAAGACAG AGTTTGATGA AAGTAACTCT CAAAGTGAAC CATCTTTAAG CCCAGAATCT 
CCTGCCAGCG TTCAAGGTGC GCTAGACTTT GAGCACATTG AAGAGCAGGC GGAGGGTATT 
TTTGGAGGAA GTGAAGAGAA CACCCCGCTG GATCTTGATG ATCATGGTGG ACGGACATTT 
CTGAGGGTCC TGCTTCACCT GACCATGCAT GACTATCCTC CGCTGGTATC TCGAGCTCTT 
CACCTGCTCT TCAGACACTT TAGCCAACGC CAGGAGGTCC TACAGGCCTT CAAACAGGTT 
CAGCTCCTGG TGACCAGTCA GGATGTGGAA AACTATAAAC AGATCAAGTC AGATTTGGAC 
CAGCTGAGGT CAATAGTAGA GAAATCAGAG TTGTGGGTGT ATAAGAGACA AGGCTCAGAC 
TCAGGACTGG ATGCTGGAGA AGTCACCCCT GAAGCCCACC ACAAGGGTGT CATCAACTCG 
AGTAGATCAA ACAAACCCAA AGTGGAAAGT ACCAGCAGCT CGAACTACAG AGTCGTGAAA 
GAGATCCTGC TGAGGCTGAG CAAACTGTGT GTTTTGGAGG GCATCTCTGG CAAGAAGAAT 
AAGAAACAGC AGCAGCGGCT CCTCAGGAAC ATGGGAGCTC ACAGTGTGGT CCTCGAACTG 
TTGCAAATCC CTTATGAGAA GGGGGAGGAT GTCCAGATGC AGGAGATCAT GACCTTAGCA 
CATCAGTTCC TTCAAAACTT CTGTGCTGGA AATCAGCAGA ACCAGGCACT TCTACACAAA 
CACATCAACC TGTTCCTCAA CCCTGGGATC CTGGAGGCGG TGACCATGCA GCATATCTTC 
ATGAATAATT TCCAGTTGTG TAGTGAGATT AATGAGCGGG TCGTGCAGCA TTTTGTGCAC 
TGCATCGAGA CACACGGCCG CAGCGTGCAC TACCTCAAGT TCCTGCAGAC CATTGTCAAG 
GCAGAGAACA AGTTCATCAA GAAATGCCAA GACATCGTCA TGGCTGAGCT GGTGACTGCA 
GGTGAGGATG TGCTGGTGTT CTATAATGAC CGGGCGTCTT TTCAGTCATT GGTTCAGATG 
ATGCGTCTTG AGCGGGAACG ACTTGATGAG AGCAGTGCAC TCAGGTATCA CATTCACCTG 
GTGGAGTTGC TGGCTGTGTG CACTGAAGGC AAGAACGTCT ACACTGAGAT CAAATGCAAC 
TCGCTGCTCC CTCTCGATGA TATTGTACGT GTGGTCACAC ATGAGGACTG CATCCCTGAG 
GTGAAGATGG CCTACGTGAA CTTCCTGAAT CACTGTTATG TTGATACAGA GGTTGAGATG 
AAGGAGATCT ACACCAGCAA TCACATGTGG AAACTTTTTG ATGATTTTCT GGTGGACGTC 
TGCAGAGTGT GCAATAACAC AAGTGACCGT AAACACGCTG ACACAGTTCT GGAGAGATAC 
GTTACAGAGA CCATAATGAG CATCGTCACC ACCTTTTTCA GCTCACCATT CTCAGACCAG 
AGCACAAGTT TGCAGACCCG GCAGCCGGTG TTTGTGCAGC TGCTGCAGGG AGTGTTTCGT 
GTATATCACT GCAACTGGCT TCTCCCAGGT CAGAAGGGGA ATGTGGAAGC CTGCATCAAA 
GTGCTGAGTG ACGTGGCCAA AGGCAGAGCC ATAGCCATTC CTGTGGATCT GGACTGTCAG 
GTGAATAATC TGTTCATGAA GTCCAACAAC ATTGTGCAGA AAACAGCCCT CAGCTGGAGA 
CTCTCTGTCC GTAACGCGAC TCGGAGAGAC TCTGTTCTGA CCACCTCTCG AGACTATCGC 
AACATCATTG AAAGACTACA GGATATAGTG TCTGCATTGG AGGAGCGGTT GAGGCCTCTG 
GTTCAGGCTG AGTTGTCAGT GTTGGTGGAT GTTCTCCATC GCCCAGAGCT GCTGTTTCCA 
GAGCACACTG AAGCCCACCG CAAGTGTGAG AGTGGAGGCT TCATCTGCAA GTTGATAAAA 
CACACTAAGC AGCTTCTAGA AGAAAACGAA GAGAGGCTTT GCATCAAAGT CCTTCAGACA 
CTCCGAGAGA TGATGACCAA AGACAGAGGA TATGGAGAGA AGGGGGAGGC CCTTCGGCAG 
CTGCTCGTCA ATCGTTACTA TGGCTTCAGG AGTGGTGGGC GGAGAGAAAG TCTGACCTCG 
TTTGGTAACT CCACCCTAAC TCCTGTCGGG CCAATCAAGA GCCAGCCAGG GGGGTTGAGC 
AGGGCTGAGA TGTCATTAAT GGAGGTGCAG TGTCATCTAG ACCGTGAAGG GGCATCGGAT 
CTTGTCATTG ATCTCATCAT GAACACTACC AGCGACCGCG TCTTCCATGA AAGCATTCTG 
TTGGCCATCG CACTGTTGGA GGGCGGAAAC ACCATCATAC AGCATTCCTT CTTCAAACGT 
TTAACAGAAG ACAAAAATTC AGAGAAGTTT TTCAGGGTGT TTTATGACAG AATGAAAGTG 
GCTCAGGTGG AGATCAAAGC CACGGTTACT GTCAACACTA GTGACCTCGG CAACAAGCGG 
CGTGACGACA ACACTCCAGA CAAAGATACT CCACAACGCC GCAGAGGTAA AGACTCCGGT 
GTGGTGGTGA CAGATGATGC TCGTGAGCAG CTGTTGGAGG CTTCAGCTGC GACTAAGAAG 
GCCTTTGGTT CATATCGTCG AGATGCTGAT CCCGAGGAGA CGTTTGGCAC GGCTGATGGA 
GATAAAGGAG GTGGAGATAA AGGCACAGAG CAGGGCGAGA TGAGCCCTGT CATCCTCATA 
ATGCAGCCAA TCCTGCGCTT CCTGCAGCTG CTGTGCGAAA ACCACAATCG AGACCTGCAG 
AATTTTCTGC GCTGTCAGAA CAATAAAAAC AACTATAATC TGGTGTGTGA GACGCTGCAG 
TTCCTGGACT GTATCTGTGG CAGCACAACA GGAGGCCTGG GGCTGTTAGG ACTCTACATC 
AACCAGCATA ACGTAGCGCT CATTAATCAG ACTGTAGAGA GCCTCACTGA ATACTGTCAA 
GGTCCCTGCC ATGACAATCA GAATTGCATT GCCACCCATG AGTCTAATGG CATCGACATC 
ATTATTGCAC TGATTCTGAA TGATATTAAT CCACTGGGCA GGAAGAGAAT GGATCTGGTT 
CTAGAACTAA AGAATAATGC ATCTAAGCTG CTGCTGGCCA TCATGGAGAG CCGTCATGAC 
AGTGAGAATG CAGAAAGAAT TCTGTACAAC ATGAGACCAA AAGAACTAGT GGAGGTGATG 
AAAATGGCCT ACCAGCAGGG GGAGGCAGAG TTTGAGGATG AGGAACAGGA GAATGGAGAG 
GATCATGCAG CATCGCCTCG CAATGTTGGA CACAACATCT ATATTCTTGC ACATCAGCTG 
TCGCGTCACA ATAAGGAATT GCAAATACTA CTGAAACCAG GTGGAGAGGA CCAGGCCCTG 
GAGTATTACA CAAAACACAC CTCACAGATA GAGATAGTTC GACAGGATCG CACTATGGAA 
CAGATTGTGT TTCCTGTACC AAATATCTGC TCTTTTCTGA CAAATGAGTC CAAGCTGCGT 
GTGTACTATG GTACAGAGAG AGACGAGCAA GGCAGTAAAA TAAATGACTT CTTCTTGCAT 
GCAGACGACC TCTTTAATGA AATGAGGTGG CAGAAAAAAC TAAGAGCTCA GCCAGTGCTG 
TACTGGTGCA GCAGGAACAT GTCGTTTTGG AGTAACGTTT CCTTTAATCT GGCGGTGCTC 
ATTAATTTGC TGGTGGCATT TTTCTACCCT CTGGATGGAG TGAGCGAAAG CCAGCTGGAA 
CCTTCTCTGT CTCTGTTGCT GTGGGTTTGT TTGTTGGGCT CACTGGGCTT CGTGTTGATG 
TCCCCGAGGC CAAATGCAGT GCGGGTGCTG GTCATCTCCA CTGTCCTGCA GTTGGGCTTC 
TCTGTAGGGC TGCAGCACAT GCTCACACTC CTGGGAGCTT TCAATGTTTG TAATAAGATC 
GTGTTCATGC TGAGCTTTGT GGGTAACCGT GGGTCATTCA CCCGGGGTTA TCGGGTGATG 
GTCATGGATA GAGAGTTCCT GTTTCATCTG CTGTACCTTC TCATCTGCAT ACTGGGCCTT 
TTTGGACACG TCTTCTTTTA CAGTCTTCTG TTGTTTGACC TGGTGAACCG GGAAGAGACT 
CTTCTGAACG TCATCAAGAG TGTGACTCGA AACGGCCGCT CCATCGTCTT GACCGCAGTG 
CTGGGCCTCA TCCTGGTCTA CCTCTTTTCT ATTGTTGGAT ATATGTTTTT CAAAGATGAC 
TTCATCCTGG AGGTGAACCG CATCTCCAAC GGCACTCTGG AGGAGGGGGT GACTTTACCT 
GCTGGTTTTT GTGATGCGAG AAACGGGTCG TGTCTTTCTG ATGATGTTGA CGAGGATGAC 
GTGGAGCGCG CGTGTGATTC TCTATGGATG TGTATGATCA CTGTACTCAG TCATGGCCTC 
AGGAGCGGTG GAGGAGTTGG AGATGTCCTG CGCAAACCGT CTAAAGAGGA GCGTCTGTTC 
GCCGCACGGG TGGTGTACGA TCTCCTGTTC TTCTTCCTGG TGATCATCAT TGTGTTGAAT 
CTGATCTTCG GTGTGATCAT TGACACATTT GCAGATCTGA GAAGCGAGAA GCAGAGAAAA 
GAGGAGATCT TGAAGACCAC ATGCTTCATC TGTGGTCTGG AGAGAGATAA GTTTGATAAT 
AAAACGGTGA CGTTTGAAGA GCACATCAAG GAGGAGCACA ACCTGTGGCA TTACCTCTTC 
TTCATAGTGC TGGTGCGGGT CAAAGACTCC ACCGAATACA CGGGACCCGA GAGCTACGTC 
GCTCAGATGA TCAAGGAGCA CAATCTGGAC TGGTTTCCTC GTATGAGGGC GATGTCTCTG 
GTCAGCAGTG ATGGAGAAGG TGAACAGAAT GAACTGCGCA GCTTACAGGA GAAACTCGAG 
TCCACAATGA GGCTCGTCTC TAATCTTACC AATCAGCTCA CAGAGCTCAA AGAGCAGATG 
ACTGAGCAAA GGAAACACAA GCAGAGGCTC GGTCTGCTGG GAAACCCCGC CCACCTCAAC 
ATCAACCCAC AGCAGCCAGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212155.1
CDS	148..8151
Translation	MVDKLSSFLHIGDVCSLYAEGSTSGFISTLGLVDDRCVVQPDAGDLNNPPKKFRDCLFRLCPMNRYSAQKQFWKAAKPGGTSTTDTVLLNKLHHAADLEKKQNDSENKKLLGTVIQYGNVIQLLHLKSNKYLTVNKRLPALLEKNAMRVTLDAAGNEGSWFYIQPFYKLRSIGDNVVIGDKVVLNPVNAGQPLHASSHQLVDNPGCNEVNSVNCTTSWKIVLFMKWSDNQEIVLKGGDVVRLFHAEQEKFLTCDEHRKKQYVFLRTTGRQSATSATSSKALWEVEVVQHDPCRGGAGYWNSLFRFKHLATGHYLAAEMDSEHEVIRARARNPQDKVMYTLVSVPDGNDISSIFELDPTTLRGGDSLVPRNSYVRLRHLCTNTWVHSTNQPIDKEEEKPVMLRIGTSPLKEDKEAFAIVPVSPAEVRDLDFANDASKVLASIAAKLEKGTITQNERRSVTKLLEDLVYFVVDIPSSAQDVLEITVNKPNRERQKLMREQNILKQIFKLLQAPFTDSGDGPMLRLEELADQRHAPFRHICRLCYRVLRYSQQDYRKNQEYIAKQFRFMQKQIGYDVLAEDTITALLHNNRKLLEKHITAAEIDTFVSLVRKNREPRFLDYLSDLCVSMNKSIPVTQELICNAVLDPANADILIETKLVLSRFEVAGTVLGEGAEEEEEDEEEVWLFWKDSGGEVKSKSIRELAQDAKDGHTEDQEVINYYRYQLNLFARMCLDRQYLAINKISAQLDVDLILRCMSDEDLPFDLRASFCRMMLHMHVDRDPQEQVTPVKYARLWSEIPSQISIDDYDNDGTSSDEVKERFSQTMEFVENYLRDVVCQSFPFSDKEKNKLTFEVVNLARNLIYFGFYNFSDLLRLTKILLAILDCVHISTPFHIKLDREPGKGSNVMRSIHGVGELMTQVVLRGGGFLPASSHNPPDRDEVKAQSEPQKQDILVMDTKLKIIEILQFILNVRLDYRISCLLCIFKTEFDESNSQSEPSLSPESPASVQGALDFEHIEEQAEGIFGGSEENTPLDLDDHGGRTFLRVLLHLTMHDYPPLVSRALHLLFRHFSQRQEVLQAFKQVQLLVTSQDVENYKQIKSDLDQLRSIVEKSELWVYKRQGSDSGLDAGEVTPEAHHKGVINSSRSNKPKVESTSSSNYRVVKEILLRLSKLCVLEGISGKKNKKQQQRLLRNMGAHSVVLELLQIPYEKGEDVQMQEIMTLAHQFLQNFCAGNQQNQALLHKHINLFLNPGILEAVTMQHIFMNNFQLCSEINERVVQHFVHCIETHGRSVHYLKFLQTIVKAENKFIKKCQDIVMAELVTAGEDVLVFYNDRASFQSLVQMMRLERERLDESSALRYHIHLVELLAVCTEGKNVYTEIKCNSLLPLDDIVRVVTHEDCIPEVKMAYVNFLNHCYVDTEVEMKEIYTSNHMWKLFDDFLVDVCRVCNNTSDRKHADTVLERYVTETIMSIVTTFFSSPFSDQSTSLQTRQPVFVQLLQGVFRVYHCNWLLPGQKGNVEACIKVLSDVAKGRAIAIPVDLDCQVNNLFMKSNNIVQKTALSWRLSVRNATRRDSVLTTSRDYRNIIERLQDIVSALEERLRPLVQAELSVLVDVLHRPELLFPEHTEAHRKCESGGFICKLIKHTKQLLEENEERLCIKVLQTLREMMTKDRGYGEKGEALRQLLVNRYYGFRSGGRRESLTSFGNSTLTPVGPIKSQPGGLSRAEMSLMEVQCHLDREGASDLVIDLIMNTTSDRVFHESILLAIALLEGGNTIIQHSFFKRLTEDKNSEKFFRVFYDRMKVAQVEIKATVTVNTSDLGNKRRDDNTPDKDTPQRRRGKDSGVVVTDDAREQLLEASAATKKAFGSYRRDADPEETFGTADGDKGGGDKGTEQGEMSPVILIMQPILRFLQLLCENHNRDLQNFLRCQNNKNNYNLVCETLQFLDCICGSTTGGLGLLGLYINQHNVALINQTVESLTEYCQGPCHDNQNCIATHESNGIDIIIALILNDINPLGRKRMDLVLELKNNASKLLLAIMESRHDSENAERILYNMRPKELVEVMKMAYQQGEAEFEDEEQENGEDHAASPRNVGHNIYILAHQLSRHNKELQILLKPGGEDQALEYYTKHTSQIEIVRQDRTMEQIVFPVPNICSFLTNESKLRVYYGTERDEQGSKINDFFLHADDLFNEMRWQKKLRAQPVLYWCSRNMSFWSNVSFNLAVLINLLVAFFYPLDGVSESQLEPSLSLLLWVCLLGSLGFVLMSPRPNAVRVLVISTVLQLGFSVGLQHMLTLLGAFNVCNKIVFMLSFVGNRGSFTRGYRVMVMDREFLFHLLYLLICILGLFGHVFFYSLLLFDLVNREETLLNVIKSVTRNGRSIVLTAVLGLILVYLFSIVGYMFFKDDFILEVNRISNGTLEEGVTLPAGFCDARNGSCLSDDVDEDDVERACDSLWMCMITVLSHGLRSGGGVGDVLRKPSKEERLFAARVVYDLLFFFLVIIIVLNLIFGVIIDTFADLRSEKQRKEEILKTTCFICGLERDKFDNKTVTFEEHIKEEHNLWHYLFFIVLVRVKDSTEYTGPESYVAQMIKEHNLDWFPRMRAMSLVSSDGEGEQNELRSLQEKLESTMRLVSNLTNQLTELKEQMTEQRKHKQRLGLLGNPAHLNINPQQPA

Target ORF information:

RefSeq Version	XM_017356666.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio inositol 1,4,5-trisphosphate receptor, type 1a (itpr1a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356666.2

ORF Insert Sequence:

ATGGTGGACA AATTGTCTAG TTTCCTGCAC ATTGGGGACG TTTGTTCCCT TTATGCTGAA 
GGCTCTACTA GTGGCTTCAT CAGCACTCTG GGGTTGGTTG ATGACCGATG TGTTGTTCAA 
CCAGATGCCG GAGACCTGAA TAACCCACCT AAAAAGTTTC GGGACTGTCT GTTCAGACTG 
TGTCCCATGA ACAGATATTC GGCACAGAAA CAGTTCTGGA AAGCTGCCAA ACCTGGAGGA 
ACTAGCACCA CAGATACTGT CCTGCTTAAC AAACTCCATC ATGCTGCTGA TTTAGAGAAG 
AAACAAAATG ATTCTGAGAA CAAAAAGCTG CTTGGAACCG TCATTCAGTA TGGCAATGTC 
ATCCAGCTTC TCCATCTGAA GAGTAATAAA TATCTGACGG TGAACAAGCG TTTGCCGGCT 
CTTCTTGAGA AGAATGCCAT GCGTGTGACG CTGGACGCTG CAGGGAATGA AGGCTCGTGG 
TTTTACATCC AGCCCTTCTA TAAGCTGCGC TCTATAGGGG ATAATGTGGT CATTGGAGAT 
AAGGTCGTGT TAAACCCTGT CAATGCCGGA CAGCCGCTGC ACGCCAGCAG CCATCAGCTT 
GTGGATAATC CAGGCTGCAA TGAAGTGAAT TCAGTGAACT GCACCACCAG TTGGAAAATC 
GTTCTCTTCA TGAAATGGAG CGACAACCAG GAGATTGTCT TGAAAGGAGG TGACGTGGTG 
AGGCTGTTTC ATGCAGAGCA GGAGAAGTTT CTGACATGTG ATGAGCATCG GAAAAAGCAG 
TATGTGTTTC TGCGTACCAC CGGACGCCAG TCTGCAACCT CCGCCACTAG TAGCAAAGCA 
CTCTGGGAGG TGGAGGTGGT TCAGCATGAT CCCTGCCGAG GTGGAGCCGG CTACTGGAAC 
AGCCTCTTTA GGTTTAAACA CTTAGCCACC GGCCATTACC TTGCTGCAGA GATGGATTCG 
GAGCATGAAG TGATTCGGGC TCGTGCACGT AACCCTCAGG ATAAGGTGAT GTACACACTG 
GTGTCTGTTC CTGATGGAAA TGACATATCC TCCATCTTTG AGCTTGACCC TACAACTTTG 
AGGGGCGGAG ACTCACTTGT GCCAAGAAAC TCGTATGTGA GGCTCAGGCA TCTCTGCACC 
AACACGTGGG TCCACAGCAC CAACCAGCCA ATAGATAAAG AGGAAGAGAA ACCGGTCATG 
CTCAGAATAG GAACGTCTCC TCTGAAGGAA GATAAAGAGG CGTTTGCTAT AGTACCAGTC 
TCTCCAGCTG AGGTTCGAGA TCTGGATTTT GCTAATGATG CTAGTAAAGT GTTGGCTTCC 
ATTGCTGCCA AACTGGAGAA GGGCACCATT ACCCAGAATG AGAGGAGGTC AGTGACGAAG 
CTGTTGGAGG ATCTGGTGTA TTTTGTGGTG GACATACCCA GCAGTGCTCA AGATGTGCTG 
GAGATCACAG TCAACAAACC CAACAGAGAG CGACAGAAAC TCATGAGAGA GCAGAACATT 
CTTAAACAGA TCTTCAAGCT GCTGCAGGCG CCATTTACAG ACAGCGGGGA CGGCCCTATG 
TTGCGATTAG AAGAGCTCGC AGACCAGCGT CATGCACCCT TTCGACACAT TTGCAGACTC 
TGTTACAGAG TCCTACGTTA CTCACAACAG GACTATCGCA AAAATCAGGA GTACATAGCA 
AAGCAGTTCC GCTTCATGCA GAAGCAGATA GGATATGATG TGCTGGCTGA AGACACAATT 
ACAGCCCTTC TGCACAACAA CAGGAAGCTC CTAGAGAAAC ACATCACAGC AGCTGAAATA 
GATACCTTTG TCAGTCTGGT CCGCAAGAAC AGAGAGCCCA GATTTCTGGA TTACTTGTCA 
GATCTGTGTG TGTCGATGAA TAAATCCATC CCGGTCACTC AAGAGCTCAT CTGTAATGCT 
GTGCTGGACC CAGCCAATGC AGACATTCTC ATCGAAACCA AGCTTGTGTT GTCACGGTTT 
GAGGTGGCAG GAACGGTGTT GGGTGAAGGT GCGGAGGAGG AAGAGGAGGA TGAAGAGGAG 
GTCTGGCTGT TCTGGAAGGA CAGTGGAGGA GAGGTGAAGA GTAAAAGCAT ACGAGAGCTT 
GCACAGGACG CAAAAGATGG ACATACAGAG GACCAGGAAG TCATCAATTA TTACAGATAT 
CAGCTGAATT TGTTTGCTCG GATGTGTCTG GATCGACAGT ATCTTGCGAT TAACAAGATA 
TCAGCACAGC TGGATGTGGA TCTGATTCTG CGCTGCATGT CAGATGAAGA TTTGCCCTTT 
GACCTCAGAG CATCGTTCTG CCGCATGATG CTGCACATGC ATGTAGACCG TGACCCTCAG 
GAGCAGGTCA CCCCGGTCAA ATATGCTCGA CTGTGGTCTG AAATCCCATC ACAAATTTCC 
ATTGATGATT ATGATAATGA TGGGACGTCA AGTGATGAAG TGAAAGAGCG CTTCTCTCAG 
ACGATGGAGT TTGTGGAGAA TTACCTGAGG GATGTTGTCT GCCAGAGTTT CCCGTTCTCT 
GATAAGGAGA AAAACAAACT CACTTTTGAG GTGGTGAACC TGGCAAGAAA CCTGATCTAT 
TTTGGTTTCT ATAACTTCAG TGATCTGCTA AGGTTAACAA AAATCCTATT GGCAATCCTG 
GACTGTGTCC ACATAAGCAC TCCCTTCCAC ATCAAGCTGG ATAGAGAACC CGGTAAAGGA 
AGTAACGTGA TGCGCTCCAT CCATGGGGTG GGAGAGCTGA TGACACAGGT GGTGTTGAGG 
GGTGGGGGAT TTCTGCCAGC CTCCAGCCAC AATCCTCCTG ACAGAGACGA AGTGAAAGCA 
CAAAGTGAAC CTCAGAAACA GGACATACTG GTGATGGACA CTAAACTCAA GATCATCGAG 
ATCCTGCAGT TTATTCTGAA TGTGCGGTTG GACTACAGGA TCTCCTGCCT CCTCTGTATC 
TTTAAGACAG AGTTTGATGA AAGTAACTCT CAAAGTGAAC CATCTTTAAG CCCAGAATCT 
CCTGCCAGCG TTCAAGGTGC GCTAGACTTT GAGCACATTG AAGAGCAGGC GGAGGGTATT 
TTTGGAGGAA GTGAAGAGAA CACCCCGCTG GATCTTGATG ATCATGGTGG ACGGACATTT 
CTGAGGGTCC TGCTTCACCT GACCATGCAT GACTATCCTC CGCTGGTATC TCGAGCTCTT 
CACCTGCTCT TCAGACACTT TAGCCAACGC CAGGAGGTCC TACAGGCCTT CAAACAGGTT 
CAGCTCCTGG TGACCAGTCA GGATGTGGAA AACTATAAAC AGATCAAGTC AGATTTGGAC 
CAGCTGAGGT CAATAGTAGA GAAATCAGAG TTGTGGGTGT ATAAGAGACA AGGCTCAGAC 
TCAGGACTGG ATGCTGGAGA AGTCACCCCT GAAGCCCACC ACAAGGGTGT CATCAACTCG 
AGTAGATCAA ACAAACCCAA AGTGGAAAGT ACCAGCAGCT CGAACTACAG AGTCGTGAAA 
GAGATCCTGC TGAGGCTGAG CAAACTGTGT GTTTTGGAGG GCATCTCTGG CAAGAAGAAT 
AAGAAACAGC AGCAGCGGCT CCTCAGGAAC ATGGGAGCTC ACAGTGTGGT CCTCGAACTG 
TTGCAAATCC CTTATGAGAA GGGGGAGGAT GTCCAGATGC AGGAGATCAT GACCTTAGCA 
CATCAGTTCC TTCAAAACTT CTGTGCTGGA AATCAGCAGA ACCAGGCACT TCTACACAAA 
CACATCAACC TGTTCCTCAA CCCTGGGATC CTGGAGGCGG TGACCATGCA GCATATCTTC 
ATGAATAATT TCCAGTTGTG TAGTGAGATT AATGAGCGGG TCGTGCAGCA TTTTGTGCAC 
TGCATCGAGA CACACGGCCG CAGCGTGCAC TACCTCAAGT TCCTGCAGAC CATTGTCAAG 
GCAGAGAACA AGTTCATCAA GAAATGCCAA GACATCGTCA TGGCTGAGCT GGTGACTGCA 
GGTGAGGATG TGCTGGTGTT CTATAATGAC CGGGCGTCTT TTCAGTCATT GGTTCAGATG 
ATGCGTCTTG AGCGGGAACG ACTTGATGAG AGCAGTGCAC TCAGGTATCA CATTCACCTG 
GTGGAGTTGC TGGCTGTGTG CACTGAAGGC AAGAACGTCT ACACTGAGAT CAAATGCAAC 
TCGCTGCTCC CTCTCGATGA TATTGTACGT GTGGTCACAC ATGAGGACTG CATCCCTGAG 
GTGAAGATGG CCTACGTGAA CTTCCTGAAT CACTGTTATG TTGATACAGA GGTTGAGATG 
AAGGAGATCT ACACCAGCAA TCACATGTGG AAACTTTTTG ATGATTTTCT GGTGGACGTC 
TGCAGAGTGT GCAATAACAC AAGTGACCGT AAACACGCTG ACACAGTTCT GGAGAGATAC 
GTTACAGAGA CCATAATGAG CATCGTCACC ACCTTTTTCA GCTCACCATT CTCAGACCAG 
AGCACAAGTT TGCAGACCCG GCAGCCGGTG TTTGTGCAGC TGCTGCAGGG AGTGTTTCGT 
GTATATCACT GCAACTGGCT TCTCCCAGGT CAGAAGGGGA ATGTGGAAGC CTGCATCAAA 
GTGCTGAGTG ACGTGGCCAA AGGCAGAGCC ATAGCCATTC CTGTGGATCT GGACTGTCAG 
GTGAATAATC TGTTCATGAA GTCCAACAAC ATTGTGCAGA AAACAGCCCT CAGCTGGAGA 
CTCTCTGTCC GTAACGCGAC TCGGAGAGAC TCTGTTCTGA CCACCTCTCG AGACTATCGC 
AACATCATTG AAAGACTACA GGATATAGTG TCTGCATTGG AGGAGCGGTT GAGGCCTCTG 
GTTCAGGCTG AGTTGTCAGT GTTGGTGGAT GTTCTCCATC GCCCAGAGCT GCTGTTTCCA 
GAGCACACTG AAGCCCACCG CAAGTGTGAG AGTGGAGGCT TCATCTGCAA GTTGATAAAA 
CACACTAAGC AGCTTCTAGA AGAAAACGAA GAGAGGCTTT GCATCAAAGT CCTTCAGACA 
CTCCGAGAGA TGATGACCAA AGACAGAGGA TATGGAGAGA AGGGGGAGGC CCTTCGGCAG 
CTGCTCGTCA ATCGTTACTA TGGCTTCAGG AGTGGTGGGC GGAGAGAAAG TCTGACCTCG 
TTTGGTAACT CCACCCTAAC TCCTGTCGGG CCAATCAAGA GCCAGCCAGG GGGGTTGAGC 
AGGGCTGAGA TGTCATTAAT GGAGGTGCAG TGTCATCTAG ACCGTGAAGG GGCATCGGAT 
CTTGTCATTG ATCTCATCAT GAACACTACC AGCGACCGCG TCTTCCATGA AAGCATTCTG 
TTGGCCATCG CACTGTTGGA GGGCGGAAAC ACCATCATAC AGCATTCCTT CTTCAAACGT 
TTAACAGAAG ACAAAAATTC AGAGAAGTTT TTCAGGGTGT TTTATGACAG AATGAAAGTG 
GCTCAGGTGG AGATCAAAGC CACGGTTACT GTCAACACTA GTGACCTCGG CAACAAGCGG 
CGTGACGACA ACACTCCAGA CAAAGATACT CCACAACGCC GCAGAGGTAA AGACTCCGGT 
GTGGTGGTGA CAGATGATGC TCGTGAGCAG CTGTTGGAGG CTTCAGCTGC GACTAAGAAG 
GCCTTTGGTT CATATCGTCG AGATGCTGAT CCCGAGGAGA CGTTTGGCAC GGCTGATGGA 
GATAAAGGAG GTGGAGATAA AGGCACAGAG CAGGGCGAGA TGAGCCCTGT CATCCTCATA 
ATGCAGCCAA TCCTGCGCTT CCTGCAGCTG CTGTGCGAAA ACCACAATCG AGACCTGCAG 
AATTTTCTGC GCTGTCAGAA CAATAAAAAC AACTATAATC TGGTGTGTGA GACGCTGCAG 
TTCCTGGACT GTATCTGTGG CAGCACAACA GGAGGCCTGG GGCTGTTAGG ACTCTACATC 
AACCAGCATA ACGTAGCGCT CATTAATCAG ACTGTAGAGA GCCTCACTGA ATACTGTCAA 
GGTCCCTGCC ATGACAATCA GAATTGCATT GCCACCCATG AGTCTAATGG CATCGACATC 
ATTATTGCAC TGATTCTGAA TGATATTAAT CCACTGGGCA GGAAGAGAAT GGATCTGGTT 
CTAGAACTAA AGAATAATGC ATCTAAGCTG CTGCTGGCCA TCATGGAGAG CCGTCATGAC 
AGTGAGAATG CAGAAAGAAT TCTGTACAAC ATGAGACCAA AAGAACTAGT GGAGGTGATG 
AAAATGGCCT ACCAGCAGGG GGAGGCAGAG TTTGAGGATG AGGAACAGGA GAATGGAGAG 
GATCATGCAG CATCGCCTCG CAATGTTGGA CACAACATCT ATATTCTTGC ACATCAGCTG 
TCGCGTCACA ATAAGGAATT GCAAATACTA CTGAAACCAG GTGGAGAGGA CCAGGCCCTG 
GAGTATTACA CAAAACACAC CTCACAGATA GAGATAGTTC GACAGGATCG CACTATGGAA 
CAGATTGTGT TTCCTGTACC AAATATCTGC TCTTTTCTGA CAAATGAGTC CAAGCTGCGT 
GTGTACTATG GTACAGAGAG AGACGAGCAA GGCAGTAAAA TAAATGACTT CTTCTTGCAT 
GCAGACGACC TCTTTAATGA AATGAGGTGG CAGAAAAAAC TAAGAGCTCA GCCAGTGCTG 
TACTGGTGCA GCAGGAACAT GTCGTTTTGG AGTAACGTTT CCTTTAATCT GGCGGTGCTC 
ATTAATTTGC TGGTGGCATT TTTCTACCCT CTGGATGGAG TGAGCGAAAG CCAGCTGGAA 
CCTTCTCTGT CTCTGTTGCT GTGGGTTTGT TTGTTGGGCT CACTGGGCTT CGTGTTGATG 
TCCCCGAGGC CAAATGCAGT GCGGGTGCTG GTCATCTCCA CTGTCCTGCA GTTGGGCTTC 
TCTGTAGGGC TGCAGCACAT GCTCACACTC CTGGGAGCTT TCAATGTTTG TAATAAGATC 
GTGTTCATGC TGAGCTTTGT GGGTAACCGT GGGTCATTCA CCCGGGGTTA TCGGGTGATG 
GTCATGGATA GAGAGTTCCT GTTTCATCTG CTGTACCTTC TCATCTGCAT ACTGGGCCTT 
TTTGGACACG TCTTCTTTTA CAGTCTTCTG TTGTTTGACC TGGTGAACCG GGAAGAGACT 
CTTCTGAACG TCATCAAGAG TGTGACTCGA AACGGCCGCT CCATCGTCTT GACCGCAGTG 
CTGGGCCTCA TCCTGGTCTA CCTCTTTTCT ATTGTTGGAT ATATGTTTTT CAAAGATGAC 
TTCATCCTGG AGGTGAACCG CATCTCCAAC GGCACTCTGG AGGAGGGGGT GACTTTACCT 
GCTGGTTTTT GTGATGCGAG AAACGGGTCG TGTCTTTCTG ATGATGTTGA CGAGGATGAC 
GTGGAGCGCG CGTGTGATTC TCTATGGATG TGTATGATCA CTGTACTCAG TCATGGCCTC 
AGGAGCGGTG GAGGAGTTGG AGATGTCCTG CGCAAACCGT CTAAAGAGGA GCGTCTGTTC 
GCCGCACGGG TGGTGTACGA TCTCCTGTTC TTCTTCCTGG TGATCATCAT TGTGTTGAAT 
CTGATCTTCG GTGTGATCAT TGACACATTT GCAGATCTGA GAAGCGAGAA GCAGAGAAAA 
GAGGAGATCT TGAAGACCAC ATGCTTCATC TGTGGTCTGG AGAGAGATAA GTTTGATAAT 
AAAACGGTGA CGTTTGAAGA GCACATCAAG GAGGAGCACA ACCTGTGGCA TTACCTCTTC 
TTCATAGTGC TGGTGCGGGT CAAAGACTCC ACCGAATACA CGGGACCCGA GAGCTACGTC 
GCTCAGATGA TCAAGGAGCA CAATCTGGAC TGGTTTCCTC GTATGAGGGC GATGTCTCTG 
GTCAGCAGTG ATGGAGAAGG TGAACAGAAT GAACTGCGCA GCTTACAGGA GAAACTCGAG 
TCCACAATGA GGCTCGTCTC TAATCTTACC AATCAGCTCA CAGAGCTCAA AGAGCAGATG 
ACTGAGCAAA GGAAACACAA GCAGAGGCTC GGTCTGCTGG GAAACCCCGC CCACCTCAAC 
ATCAACCCAC AGCAGCCAGC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	itpr1a
Entrez Gene ID	567611
Full Name	inositol 1,4,5-trisphosphate receptor, type 1a
General protein information	Preferred Names inositol 1,4,5-trisphosphate receptor, type 1a Names inositol 1,4,5-trisphosphate receptor type 1 ITPR1b1 ITPR1b3 inositol 1,4,5-triphosphate receptor, type 1a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
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5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

itpr1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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