MTHFD1A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	MTHFD1A
Entrez Gene ID	567587
Full Name	methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1a, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase
Synonyms	si:dkey-1h6.1
General protein information	Preferred Names methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1a, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase Names C-1-tetrahydrofolate synthase, cytoplasmic MTHFD1 methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1a
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017352710.2	XP_017208199.1	C-1-tetrahydrofolate synthase, cytoplasmic isoform X2
XM_005160613.3	XP_005160670.1	C-1-tetrahydrofolate synthase, cytoplasmic isoform X3
XM_021473374.1	XP_021329049.1	C-1-tetrahydrofolate synthase, cytoplasmic isoform X1
XM_690886.7	XP_695978.4	C-1-tetrahydrofolate synthase, cytoplasmic isoform X1
XM_690886.8	XP_695978.4	C-1-tetrahydrofolate synthase, cytoplasmic isoform X1
XM_021473375.1	XP_021329050.1	C-1-tetrahydrofolate synthase, cytoplasmic isoform X2
XM_017352710.1	XP_017208199.1	C-1-tetrahydrofolate synthase, cytoplasmic isoform X2

Pathways from BioSystems
Gene Ontology

The following MTHFD1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTHFD1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa24899	XM_005160613.3 Latest version!	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1a, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (mthfd1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa24898	XM_690886.8 Latest version!	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1a, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (mthfd1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa24898	XM_021473374.1 Latest version!	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1a, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (mthfd1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa56730	XM_017352710.2 Latest version!	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1a, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (mthfd1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa56730	XM_021473375.1 Latest version!	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1a, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (mthfd1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
ODa24898	XM_690886.7	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1a, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (mthfd1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa56730	XM_017352710.1	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1a, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (mthfd1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MTHFD1A ( XM_005160613.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005160670.1 Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1a, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (mthfd1a), transcript variant X3, mRNA.

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CloneID	ODa24899
Clone ID Related Accession (Same CDS sequence)	XM_005160613.3
Accession Version	XM_005160613.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1995bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	C-1-tetrahydrofolate synthase, cytoplasmic isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160613.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTCTG TCAGTGCTGC TGTGCTACGG CGAGGTGTTT TAAGGTACAG ATGGGACAGA 
TGCTGTGCGC TTGCAACAGT TGTTTCTGGA AACAAGACCT CACAGCAGGT GAAACAGCGG 
CTGAAGGAGG AGGTGGAGCA GATGAAGAGT CAGTTTCCAG GTTTCAGGCC TGGTCTTGTG 
GTGCTGCAGG TTGGAGACAG AGAAGACTCA AACCTCTACA TCAGTATGAA GCTCAAAGCT 
GCGGCTGAGA TTGGAATCAA TGCGAACCAC ATCAGGCTTC CTGAAACAGC CACTGAAAAT 
GAAGTCCTAC GCAACATAGT TGCGGTGAAT GAGAATCCTG CATTTCATGG GCTCATTGTG 
CAGCTGCCGC TCGACTCCAT CAACCCAATA AACACTGAGA AAATCACCAA TGCTGTCGCT 
CCAGAGAAAG ATGTTGATGG CTTGACCAGT ATAAATGCGG GGAAGCTGTC TCGTGGAGAC 
CTGGGCGACT GCTTCATCCC ATGCACTCCT AATGGCTGTA TGGAGCTTAT CAGCCAGACT 
GGTGTGAGTG TGATGGGGAA GAATGCTGTC GTGATCGGCC GCAGCAAGAT TGTTGGAGCC 
CCAATGCACG ATCTGCTCTT GTGGAGCCAC GCAACAGTGA CCACCTGCCA CTCGAAGACC 
CCTGACCTCG CCGAACAGGT GGGCAAAGCA GATATTTTGG TTGTGGGTGC TGGCAGAGCA 
GAGATGGTGA AGGGCGAGTG GGTGAAGGAG GGGGCCGTGG TCATTGACTG CGGCATCAAT 
CATATACCAG ATGAGAGTAA AGCTAGCGGG AAGCGGGTGG TGGGTGATGT GCATTACCCC 
TCAGCTAAAC AGCGAGCTGG ATTCATTACC CCAGTGCCTG GTGGAGTCGG ACCCATGACT 
GTGGCTATGC TGATGAAGAA TACAGTTGAA AGTGCGAAGA GGTTTCTGCA AACACACACT 
CCTGGCAAGT GGAACTTAGT GTACACAAAG AAGAGACCCC ATGAGCCTCA GCCCAGCGAT 
GCTGCAATCT CCTCTTCATG TGAAAGCAAA CCAGTAGTTC AGCTCGCCAG AGAATTGGGA 
TTGTTTTCTG ATGAAGTAGA GTCATACGGC AGGAGCAGGG CTAAGATCCG ACTGGATGCT 
TTGAAGCGCA TGGAAAAACA GCCGAATGGG AAATATGTGG TTGTCACCGG AGTTACTTCT 
TCTCCACTGG GTGAGGGAAA GCGCACCACT GCTCTCGGAC TGGCTCAAGC TTTAGGGGCT 
CACCTGAATA CCAACGCTTT CGCTTGTGTT CAAAAGTCCT CACTGGAAAC CTGTTTTGGT 
GCTAGAGGGC CTGCTGTTGG AGGAGGATAC TCTCAAATTA TCCCACTGGA GGAGGTCAGC 
CTTCAGCCTT CAGGCCAGAA TGAGCTCGTC TCTGCTGCCG GTAGACTGGT GATGGACACT 
GTTAAAGCAT ATGCTCACTA TGAGACCAAA CTCTCGGAAA AGGCTTTGTT TGATTTGTTG 
GTTCCTCTCA GAGAGGGTCA AAGGTGCGTC TCCTTGGCTC AGCTTAATCG ACTGAAGAAA 
CTGGCCATTG AGAAATCTGA CCCCTTGACT CTCACAGAGA GAGAGATAAG ACGTTTTATC 
CGATTGGACA TTGACACAGA CTCTGCAACG GATTCATTCT CCTTGGAAAA TGAGATGATG 
GCTGTCCTGT CACTCAGCAG CAGTGAGGAG GAGATGCAGC ACAGACTGGC CAACATCATA 
GTAGCCACAA ACAAATCTGG AGATCCTATC ACTACCGAGG ATTTAGTCAA ATGTCAGTGG 
TCCTTTGGCT ATGTTATTGA AAGAAGCCCT GAAACCTGTC TTGATGCAAA CTGTCGAGGG 
TACACCTGTG TTTCTTCACA CCAGCCCATT AGTCAATGTT GCCCAAGGCG GTCCTTCCAT 
TCTGGCAGAC AAAATGGCTT TAAAAATTGT TGGATCAGAG GGATTTGTAG TCACAGAGTC 
GGGTCATGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160670.1
CDS	550..2544
Translation	MFSVSAAVLRRGVLRYRWDRCCALATVVSGNKTSQQVKQRLKEEVEQMKSQFPGFRPGLVVLQVGDREDSNLYISMKLKAAAEIGINANHIRLPETATENEVLRNIVAVNENPAFHGLIVQLPLDSINPINTEKITNAVAPEKDVDGLTSINAGKLSRGDLGDCFIPCTPNGCMELISQTGVSVMGKNAVVIGRSKIVGAPMHDLLLWSHATVTTCHSKTPDLAEQVGKADILVVGAGRAEMVKGEWVKEGAVVIDCGINHIPDESKASGKRVVGDVHYPSAKQRAGFITPVPGGVGPMTVAMLMKNTVESAKRFLQTHTPGKWNLVYTKKRPHEPQPSDAAISSSCESKPVVQLARELGLFSDEVESYGRSRAKIRLDALKRMEKQPNGKYVVVTGVTSSPLGEGKRTTALGLAQALGAHLNTNAFACVQKSSLETCFGARGPAVGGGYSQIIPLEEVSLQPSGQNELVSAAGRLVMDTVKAYAHYETKLSEKALFDLLVPLREGQRCVSLAQLNRLKKLAIEKSDPLTLTEREIRRFIRLDIDTDSATDSFSLENEMMAVLSLSSSEEEMQHRLANIIVATNKSGDPITTEDLVKCQWSFGYVIERSPETCLDANCRGYTCVSSHQPISQCCPRRSFHSGRQNGFKNCWIRGICSHRVGSWS

Target ORF information:

RefSeq Version	XM_005160613.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1a, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (mthfd1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160613.3

ORF Insert Sequence:

ATGTTTTCTG TCAGTGCTGC TGTGCTACGG CGAGGTGTTT TAAGGTACAG ATGGGACAGA 
TGCTGTGCGC TTGCAACAGT TGTTTCTGGA AACAAGACCT CACAGCAGGT GAAACAGCGG 
CTGAAGGAGG AGGTGGAGCA GATGAAGAGT CAGTTTCCAG GTTTCAGGCC TGGTCTTGTG 
GTGCTGCAGG TTGGAGACAG AGAAGACTCA AACCTCTACA TCAGTATGAA GCTCAAAGCT 
GCGGCTGAGA TTGGAATCAA TGCGAACCAC ATCAGGCTTC CTGAAACAGC CACTGAAAAT 
GAAGTCCTAC GCAACATAGT TGCGGTGAAT GAGAATCCTG CATTTCATGG GCTCATTGTG 
CAGCTGCCGC TCGACTCCAT CAACCCAATA AACACTGAGA AAATCACCAA TGCTGTCGCT 
CCAGAGAAAG ATGTTGATGG CTTGACCAGT ATAAATGCGG GGAAGCTGTC TCGTGGAGAC 
CTGGGCGACT GCTTCATCCC ATGCACTCCT AATGGCTGTA TGGAGCTTAT CAGCCAGACT 
GGTGTGAGTG TGATGGGGAA GAATGCTGTC GTGATCGGCC GCAGCAAGAT TGTTGGAGCC 
CCAATGCACG ATCTGCTCTT GTGGAGCCAC GCAACAGTGA CCACCTGCCA CTCGAAGACC 
CCTGACCTCG CCGAACAGGT GGGCAAAGCA GATATTTTGG TTGTGGGTGC TGGCAGAGCA 
GAGATGGTGA AGGGCGAGTG GGTGAAGGAG GGGGCCGTGG TCATTGACTG CGGCATCAAT 
CATATACCAG ATGAGAGTAA AGCTAGCGGG AAGCGGGTGG TGGGTGATGT GCATTACCCC 
TCAGCTAAAC AGCGAGCTGG ATTCATTACC CCAGTGCCTG GTGGAGTCGG ACCCATGACT 
GTGGCTATGC TGATGAAGAA TACAGTTGAA AGTGCGAAGA GGTTTCTGCA AACACACACT 
CCTGGCAAGT GGAACTTAGT GTACACAAAG AAGAGACCCC ATGAGCCTCA GCCCAGCGAT 
GCTGCAATCT CCTCTTCATG TGAAAGCAAA CCAGTAGTTC AGCTCGCCAG AGAATTGGGA 
TTGTTTTCTG ATGAAGTAGA GTCATACGGC AGGAGCAGGG CTAAGATCCG ACTGGATGCT 
TTGAAGCGCA TGGAAAAACA GCCGAATGGG AAATATGTGG TTGTCACCGG AGTTACTTCT 
TCTCCACTGG GTGAGGGAAA GCGCACCACT GCTCTCGGAC TGGCTCAAGC TTTAGGGGCT 
CACCTGAATA CCAACGCTTT CGCTTGTGTT CAAAAGTCCT CACTGGAAAC CTGTTTTGGT 
GCTAGAGGGC CTGCTGTTGG AGGAGGATAC TCTCAAATTA TCCCACTGGA GGAGGTCAGC 
CTTCAGCCTT CAGGCCAGAA TGAGCTCGTC TCTGCTGCCG GTAGACTGGT GATGGACACT 
GTTAAAGCAT ATGCTCACTA TGAGACCAAA CTCTCGGAAA AGGCTTTGTT TGATTTGTTG 
GTTCCTCTCA GAGAGGGTCA AAGGTGCGTC TCCTTGGCTC AGCTTAATCG ACTGAAGAAA 
CTGGCCATTG AGAAATCTGA CCCCTTGACT CTCACAGAGA GAGAGATAAG ACGTTTTATC 
CGATTGGACA TTGACACAGA CTCTGCAACG GATTCATTCT CCTTGGAAAA TGAGATGATG 
GCTGTCCTGT CACTCAGCAG CAGTGAGGAG GAGATGCAGC ACAGACTGGC CAACATCATA 
GTAGCCACAA ACAAATCTGG AGATCCTATC ACTACCGAGG ATTTAGTCAA ATGTCAGTGG 
TCCTTTGGCT ATGTTATTGA AAGAAGCCCT GAAACCTGTC TTGATGCAAA CTGTCGAGGG 
TACACCTGTG TTTCTTCACA CCAGCCCATT AGTCAATGTT GCCCAAGGCG GTCCTTCCAT 
TCTGGCAGAC AAAATGGCTT TAAAAATTGT TGGATCAGAG GGATTTGTAG TCACAGAGTC 
GGGTCATGGA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFD1A ( XM_021473374.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021329049.1 Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1a, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (mthfd1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa24898
Clone ID Related Accession (Same CDS sequence)	XM_021473374.1 , XM_690886.7 , XM_690886.8
Accession Version	XM_021473374.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	C-1-tetrahydrofolate synthase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTCTG TCAGTGCTGC TGTGCTACGG CGAGGTGTTT TAAGGTACAG ATGGGACAGA 
TGCTGTGCGC TTGCAACAGT TGTTTCTGGA AACAAGACCT CACAGCAGGT GAAACAGCGG 
CTGAAGGAGG AGGTGGAGCA GATGAAGAGT CAGTTTCCAG GTTTCAGGCC TGGTCTTGTG 
GTGCTGCAGG TTGGAGACAG AGAAGACTCA AACCTCTACA TCAGTATGAA GCTCAAAGCT 
GCGGCTGAGA TTGGAATCAA TGCGAACCAC ATCAGGCTTC CTGAAACAGC CACTGAAAAT 
GAAGTCCTAC GCAACATAGT TGCGGTGAAT GAGAATCCTG CATTTCATGG GCTCATTGTG 
CAGCTGCCGC TCGACTCCAT CAACCCAATA AACACTGAGA AAATCACCAA TGCTGTCGCT 
CCAGAGAAAG ATGTTGATGG CTTGACCAGT ATAAATGCGG GGAAGCTGTC TCGTGGAGAC 
CTGGGCGACT GCTTCATCCC ATGCACTCCT AATGGCTGTA TGGAGCTTAT CAGCCAGACT 
GGTGTGAGTG TGATGGGGAA GAATGCTGTC GTGATCGGCC GCAGCAAGAT TGTTGGAGCC 
CCAATGCACG ATCTGCTCTT GTGGAGCCAC GCAACAGTGA CCACCTGCCA CTCGAAGACC 
CCTGACCTCG CCGAACAGGT GGGCAAAGCA GATATTTTGG TTGTGGGTGC TGGCAGAGCA 
GAGATGGTGA AGGGCGAGTG GGTGAAGGAG GGGGCCGTGG TCATTGACTG CGGCATCAAT 
CATATACCAG ATGAGAGTAA AGCTAGCGGG AAGCGGGTGG TGGGTGATGT GCATTACCCC 
TCAGCTAAAC AGCGAGCTGG ATTCATTACC CCAGTGCCTG GTGGAGTCGG ACCCATGACT 
GTGGCTATGC TGATGAAGAA TACAGTTGAA AGTGCGAAGA GGTTTCTGCA AACACACACT 
CCTGGCAAGT GGAACTTAGT GTACACAAAG AAGAGACCCC ATGAGCCTCA GCCCAGCGAT 
GCTGCAATCT CCTCTTCATG TGAAAGCAAA CCAGTAGTTC AGCTCGCCAG AGAATTGGGA 
TTGTTTTCTG ATGAAGTAGA GTCATACGGC AGGAGCAGGG CTAAGATCCG ACTGGATGCT 
TTGAAGCGCA TGGAAAAACA GCCGAATGGG AAATATGTGG TTGTCACCGG AGTTACTTCT 
TCTCCACTGG GTGAGGGAAA GCGCACCACT GCTCTCGGAC TGGCTCAAGC TTTAGGGGCT 
CACCTGAATA CCAACGCTTT CGCTTGTGTT CAAAAGTCCT CACTGGAAAC CTGTTTTGGT 
GCTAGAGGGC CTGCTGTTGG AGGAGGATAC TCTCAAATTA TCCCACTGGA GGAGGTCAGC 
CTTCAGCCTT CAGGCCAGAA TGAGCTCGTC TCTGCTGCCG GTAGACTGGT GATGGACACT 
GTTAAAGCAT ATGCTCACTA TGAGACCAAA CTCTCGGAAA AGGCTTTGTT TGATTTGTTG 
GTTCCTCTCA GAGAGGGTCA AAGGTGCGTC TCCTTGGCTC AGCTTAATCG ACTGAAGAAA 
CTGGCCATTG AGAAATCTGA CCCCTTGACT CTCACAGAGA GAGAGATAAG ACGTTTTATC 
CGATTGGACA TTGACACAGA CTCTGCAACG GATTCATTCT CCTTGGAAAA TGAGATGATG 
GCTGTCCTGT CACTCAGCAG CAGTGAGGAG GAGATGCAGC ACAGACTGGC CAACATCATA 
GTAGCCACAA ACAAATCTGG AGATCCTATC ACTACCGAGG ATTTAAATGT CAGTGGTCCT 
TTGGCTATGT TATTGAAAGA AGCCCTGAAA CCTGTCTTGA TGCAAACTGT CGAGGGTACA 
CCTGTGTTTC TTCACACCAG CCCATTAGTC AATGTTGCCC AAGGCGGTCC TTCCATTCTG 
GCAGACAAAA TGGCTTTAAA AATTGTTGGA TCAGAGGGAT TTGTAGTCAC AGAGTCGGGT 
CATGGAGCTG ATGTTGGCTT GGAGAAGTTT TTCAACATTA AATGCCTCTA CTCAGGTCTT 
CAGCCAGATG TGCTAGTGAT GGTGGCAACC ATTCGTACAT TGAAGATGCA AGGTGGAGCA 
CCTCCTATCC AAGCAGGTTT GCCCCTTCCA AAAGAGTACT TACAAGAGAA CCTCAAGCAG 
CTGGAAAGAG GCTGTTGTCA TATGAGGAGG CAGGTGGAAA AAGCACAAGC ATTCGGCTTG 
CCGGTGATCG TAGCTGTCAA CAAATTCAGC TCTGATACTG ACGCTGAGCT GGAGCTGGTA 
TGTGGCCAGG CGAGGCAGGC TGGTGCCTTT GATGCAGTGC AGTGCAGCAG CTGGTCTGAA 
GGGGGCGCTG GAGCTCTGGC TCTGGCCGAG ACGGTTCAGA GAGCAGCAAA ACTACCTGCC 
TGCTTCAAGT TCCTCTATGA CTTGCAGACT CCAGCCTCTG ATAAGTTAAG GACTGTAGCA 
CAGAAGATGT ACGGTCTGAA AGACGTCAAG CTGTCTCCAA AAGCAAAGGA AAAACTTGCA 
CTTTATACAA AACAGGGCTT TGAAAATTTG CCGGTTTGCA TAGCCAAGAC ACCGCTGTCC 
TTGAGTAATG ACCCCATGAT GAAGGGGTCG TCCACTGACT TCATGCTGCC CATAACCGAT 
ATCAAAGCCA ATGCTGGAGC AGGTTTCCTT TGCTGTCTGA CTGGCTCGAC AAATGCAGAA 
ATGGATGATC CCATGTGGCC CTGTTTCCAT GGCAACAACC TTTACAGTGA CAGCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329049.1
CDS	733..3492
Translation	MFSVSAAVLRRGVLRYRWDRCCALATVVSGNKTSQQVKQRLKEEVEQMKSQFPGFRPGLVVLQVGDREDSNLYISMKLKAAAEIGINANHIRLPETATENEVLRNIVAVNENPAFHGLIVQLPLDSINPINTEKITNAVAPEKDVDGLTSINAGKLSRGDLGDCFIPCTPNGCMELISQTGVSVMGKNAVVIGRSKIVGAPMHDLLLWSHATVTTCHSKTPDLAEQVGKADILVVGAGRAEMVKGEWVKEGAVVIDCGINHIPDESKASGKRVVGDVHYPSAKQRAGFITPVPGGVGPMTVAMLMKNTVESAKRFLQTHTPGKWNLVYTKKRPHEPQPSDAAISSSCESKPVVQLARELGLFSDEVESYGRSRAKIRLDALKRMEKQPNGKYVVVTGVTSSPLGEGKRTTALGLAQALGAHLNTNAFACVQKSSLETCFGARGPAVGGGYSQIIPLEEVSLQPSGQNELVSAAGRLVMDTVKAYAHYETKLSEKALFDLLVPLREGQRCVSLAQLNRLKKLAIEKSDPLTLTEREIRRFIRLDIDTDSATDSFSLENEMMAVLSLSSSEEEMQHRLANIIVATNKSGDPITTEDLNVSGPLAMLLKEALKPVLMQTVEGTPVFLHTSPLVNVAQGGPSILADKMALKIVGSEGFVVTESGHGADVGLEKFFNIKCLYSGLQPDVLVMVATIRTLKMQGGAPPIQAGLPLPKEYLQENLKQLERGCCHMRRQVEKAQAFGLPVIVAVNKFSSDTDAELELVCGQARQAGAFDAVQCSSWSEGGAGALALAETVQRAAKLPACFKFLYDLQTPASDKLRTVAQKMYGLKDVKLSPKAKEKLALYTKQGFENLPVCIAKTPLSLSNDPMMKGSSTDFMLPITDIKANAGAGFLCCLTGSTNAEMDDPMWPCFHGNNLYSDSK

Target ORF information:

RefSeq Version	XM_021473374.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1a, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (mthfd1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473374.1

ORF Insert Sequence:

ATGTTTTCTG TCAGTGCTGC TGTGCTACGG CGAGGTGTTT TAAGGTACAG ATGGGACAGA 
TGCTGTGCGC TTGCAACAGT TGTTTCTGGA AACAAGACCT CACAGCAGGT GAAACAGCGG 
CTGAAGGAGG AGGTGGAGCA GATGAAGAGT CAGTTTCCAG GTTTCAGGCC TGGTCTTGTG 
GTGCTGCAGG TTGGAGACAG AGAAGACTCA AACCTCTACA TCAGTATGAA GCTCAAAGCT 
GCGGCTGAGA TTGGAATCAA TGCGAACCAC ATCAGGCTTC CTGAAACAGC CACTGAAAAT 
GAAGTCCTAC GCAACATAGT TGCGGTGAAT GAGAATCCTG CATTTCATGG GCTCATTGTG 
CAGCTGCCGC TCGACTCCAT CAACCCAATA AACACTGAGA AAATCACCAA TGCTGTCGCT 
CCAGAGAAAG ATGTTGATGG CTTGACCAGT ATAAATGCGG GGAAGCTGTC TCGTGGAGAC 
CTGGGCGACT GCTTCATCCC ATGCACTCCT AATGGCTGTA TGGAGCTTAT CAGCCAGACT 
GGTGTGAGTG TGATGGGGAA GAATGCTGTC GTGATCGGCC GCAGCAAGAT TGTTGGAGCC 
CCAATGCACG ATCTGCTCTT GTGGAGCCAC GCAACAGTGA CCACCTGCCA CTCGAAGACC 
CCTGACCTCG CCGAACAGGT GGGCAAAGCA GATATTTTGG TTGTGGGTGC TGGCAGAGCA 
GAGATGGTGA AGGGCGAGTG GGTGAAGGAG GGGGCCGTGG TCATTGACTG CGGCATCAAT 
CATATACCAG ATGAGAGTAA AGCTAGCGGG AAGCGGGTGG TGGGTGATGT GCATTACCCC 
TCAGCTAAAC AGCGAGCTGG ATTCATTACC CCAGTGCCTG GTGGAGTCGG ACCCATGACT 
GTGGCTATGC TGATGAAGAA TACAGTTGAA AGTGCGAAGA GGTTTCTGCA AACACACACT 
CCTGGCAAGT GGAACTTAGT GTACACAAAG AAGAGACCCC ATGAGCCTCA GCCCAGCGAT 
GCTGCAATCT CCTCTTCATG TGAAAGCAAA CCAGTAGTTC AGCTCGCCAG AGAATTGGGA 
TTGTTTTCTG ATGAAGTAGA GTCATACGGC AGGAGCAGGG CTAAGATCCG ACTGGATGCT 
TTGAAGCGCA TGGAAAAACA GCCGAATGGG AAATATGTGG TTGTCACCGG AGTTACTTCT 
TCTCCACTGG GTGAGGGAAA GCGCACCACT GCTCTCGGAC TGGCTCAAGC TTTAGGGGCT 
CACCTGAATA CCAACGCTTT CGCTTGTGTT CAAAAGTCCT CACTGGAAAC CTGTTTTGGT 
GCTAGAGGGC CTGCTGTTGG AGGAGGATAC TCTCAAATTA TCCCACTGGA GGAGGTCAGC 
CTTCAGCCTT CAGGCCAGAA TGAGCTCGTC TCTGCTGCCG GTAGACTGGT GATGGACACT 
GTTAAAGCAT ATGCTCACTA TGAGACCAAA CTCTCGGAAA AGGCTTTGTT TGATTTGTTG 
GTTCCTCTCA GAGAGGGTCA AAGGTGCGTC TCCTTGGCTC AGCTTAATCG ACTGAAGAAA 
CTGGCCATTG AGAAATCTGA CCCCTTGACT CTCACAGAGA GAGAGATAAG ACGTTTTATC 
CGATTGGACA TTGACACAGA CTCTGCAACG GATTCATTCT CCTTGGAAAA TGAGATGATG 
GCTGTCCTGT CACTCAGCAG CAGTGAGGAG GAGATGCAGC ACAGACTGGC CAACATCATA 
GTAGCCACAA ACAAATCTGG AGATCCTATC ACTACCGAGG ATTTAAATGT CAGTGGTCCT 
TTGGCTATGT TATTGAAAGA AGCCCTGAAA CCTGTCTTGA TGCAAACTGT CGAGGGTACA 
CCTGTGTTTC TTCACACCAG CCCATTAGTC AATGTTGCCC AAGGCGGTCC TTCCATTCTG 
GCAGACAAAA TGGCTTTAAA AATTGTTGGA TCAGAGGGAT TTGTAGTCAC AGAGTCGGGT 
CATGGAGCTG ATGTTGGCTT GGAGAAGTTT TTCAACATTA AATGCCTCTA CTCAGGTCTT 
CAGCCAGATG TGCTAGTGAT GGTGGCAACC ATTCGTACAT TGAAGATGCA AGGTGGAGCA 
CCTCCTATCC AAGCAGGTTT GCCCCTTCCA AAAGAGTACT TACAAGAGAA CCTCAAGCAG 
CTGGAAAGAG GCTGTTGTCA TATGAGGAGG CAGGTGGAAA AAGCACAAGC ATTCGGCTTG 
CCGGTGATCG TAGCTGTCAA CAAATTCAGC TCTGATACTG ACGCTGAGCT GGAGCTGGTA 
TGTGGCCAGG CGAGGCAGGC TGGTGCCTTT GATGCAGTGC AGTGCAGCAG CTGGTCTGAA 
GGGGGCGCTG GAGCTCTGGC TCTGGCCGAG ACGGTTCAGA GAGCAGCAAA ACTACCTGCC 
TGCTTCAAGT TCCTCTATGA CTTGCAGACT CCAGCCTCTG ATAAGTTAAG GACTGTAGCA 
CAGAAGATGT ACGGTCTGAA AGACGTCAAG CTGTCTCCAA AAGCAAAGGA AAAACTTGCA 
CTTTATACAA AACAGGGCTT TGAAAATTTG CCGGTTTGCA TAGCCAAGAC ACCGCTGTCC 
TTGAGTAATG ACCCCATGAT GAAGGGGTCG TCCACTGACT TCATGCTGCC CATAACCGAT 
ATCAAAGCCA ATGCTGGAGC AGGTTTCCTT TGCTGTCTGA CTGGCTCGAC AAATGCAGAA 
ATGGATGATC CCATGTGGCC CTGTTTCCAT GGCAACAACC TTTACAGTGA CAGCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFD1A ( XM_690886.7 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_695978.4 Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1a, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (mthfd1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa24898
Clone ID Related Accession (Same CDS sequence)	XM_021473374.1 , XM_690886.7 , XM_690886.8
Accession Version	XM_690886.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	C-1-tetrahydrofolate synthase, cytoplasmic isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_690886.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTCTG TCAGTGCTGC TGTGCTACGG CGAGGTGTTT TAAGGTACAG ATGGGACAGA 
TGCTGTGCGC TTGCAACAGT TGTTTCTGGA AACAAGACCT CACAGCAGGT GAAACAGCGG 
CTGAAGGAGG AGGTGGAGCA GATGAAGAGT CAGTTTCCAG GTTTCAGGCC TGGTCTTGTG 
GTGCTGCAGG TTGGAGACAG AGAAGACTCA AACCTCTACA TCAGTATGAA GCTCAAAGCT 
GCGGCTGAGA TTGGAATCAA TGCGAACCAC ATCAGGCTTC CTGAAACAGC CACTGAAAAT 
GAAGTCCTAC GCAACATAGT TGCGGTGAAT GAGAATCCTG CATTTCATGG GCTCATTGTG 
CAGCTGCCGC TCGACTCCAT CAACCCAATA AACACTGAGA AAATCACCAA TGCTGTCGCT 
CCAGAGAAAG ATGTTGATGG CTTGACCAGT ATAAATGCGG GGAAGCTGTC TCGTGGAGAC 
CTGGGCGACT GCTTCATCCC ATGCACTCCT AATGGCTGTA TGGAGCTTAT CAGCCAGACT 
GGTGTGAGTG TGATGGGGAA GAATGCTGTC GTGATCGGCC GCAGCAAGAT TGTTGGAGCC 
CCAATGCACG ATCTGCTCTT GTGGAGCCAC GCAACAGTGA CCACCTGCCA CTCGAAGACC 
CCTGACCTCG CCGAACAGGT GGGCAAAGCA GATATTTTGG TTGTGGGTGC TGGCAGAGCA 
GAGATGGTGA AGGGCGAGTG GGTGAAGGAG GGGGCCGTGG TCATTGACTG CGGCATCAAT 
CATATACCAG ATGAGAGTAA AGCTAGCGGG AAGCGGGTGG TGGGTGATGT GCATTACCCC 
TCAGCTAAAC AGCGAGCTGG ATTCATTACC CCAGTGCCTG GTGGAGTCGG ACCCATGACT 
GTGGCTATGC TGATGAAGAA TACAGTTGAA AGTGCGAAGA GGTTTCTGCA AACACACACT 
CCTGGCAAGT GGAACTTAGT GTACACAAAG AAGAGACCCC ATGAGCCTCA GCCCAGCGAT 
GCTGCAATCT CCTCTTCATG TGAAAGCAAA CCAGTAGTTC AGCTCGCCAG AGAATTGGGA 
TTGTTTTCTG ATGAAGTAGA GTCATACGGC AGGAGCAGGG CTAAGATCCG ACTGGATGCT 
TTGAAGCGCA TGGAAAAACA GCCGAATGGG AAATATGTGG TTGTCACCGG AGTTACTTCT 
TCTCCACTGG GTGAGGGAAA GCGCACCACT GCTCTCGGAC TGGCTCAAGC TTTAGGGGCT 
CACCTGAATA CCAACGCTTT CGCTTGTGTT CAAAAGTCCT CACTGGAAAC CTGTTTTGGT 
GCTAGAGGGC CTGCTGTTGG AGGAGGATAC TCTCAAATTA TCCCACTGGA GGAGGTCAGC 
CTTCAGCCTT CAGGCCAGAA TGAGCTCGTC TCTGCTGCCG GTAGACTGGT GATGGACACT 
GTTAAAGCAT ATGCTCACTA TGAGACCAAA CTCTCGGAAA AGGCTTTGTT TGATTTGTTG 
GTTCCTCTCA GAGAGGGTCA AAGGTGCGTC TCCTTGGCTC AGCTTAATCG ACTGAAGAAA 
CTGGCCATTG AGAAATCTGA CCCCTTGACT CTCACAGAGA GAGAGATAAG ACGTTTTATC 
CGATTGGACA TTGACACAGA CTCTGCAACG GATTCATTCT CCTTGGAAAA TGAGATGATG 
GCTGTCCTGT CACTCAGCAG CAGTGAGGAG GAGATGCAGC ACAGACTGGC CAACATCATA 
GTAGCCACAA ACAAATCTGG AGATCCTATC ACTACCGAGG ATTTAAATGT CAGTGGTCCT 
TTGGCTATGT TATTGAAAGA AGCCCTGAAA CCTGTCTTGA TGCAAACTGT CGAGGGTACA 
CCTGTGTTTC TTCACACCAG CCCATTAGTC AATGTTGCCC AAGGCGGTCC TTCCATTCTG 
GCAGACAAAA TGGCTTTAAA AATTGTTGGA TCAGAGGGAT TTGTAGTCAC AGAGTCGGGT 
CATGGAGCTG ATGTTGGCTT GGAGAAGTTT TTCAACATTA AATGCCTCTA CTCAGGTCTT 
CAGCCAGATG TGCTAGTGAT GGTGGCAACC ATTCGTACAT TGAAGATGCA AGGTGGAGCA 
CCTCCTATCC AAGCAGGTTT GCCCCTTCCA AAAGAGTACT TACAAGAGAA CCTCAAGCAG 
CTGGAAAGAG GCTGTTGTCA TATGAGGAGG CAGGTGGAAA AAGCACAAGC ATTCGGCTTG 
CCGGTGATCG TAGCTGTCAA CAAATTCAGC TCTGATACTG ACGCTGAGCT GGAGCTGGTA 
TGTGGCCAGG CGAGGCAGGC TGGTGCCTTT GATGCAGTGC AGTGCAGCAG CTGGTCTGAA 
GGGGGCGCTG GAGCTCTGGC TCTGGCCGAG ACGGTTCAGA GAGCAGCAAA ACTACCTGCC 
TGCTTCAAGT TCCTCTATGA CTTGCAGACT CCAGCCTCTG ATAAGTTAAG GACTGTAGCA 
CAGAAGATGT ACGGTCTGAA AGACGTCAAG CTGTCTCCAA AAGCAAAGGA AAAACTTGCA 
CTTTATACAA AACAGGGCTT TGAAAATTTG CCGGTTTGCA TAGCCAAGAC ACCGCTGTCC 
TTGAGTAATG ACCCCATGAT GAAGGGGTCG TCCACTGACT TCATGCTGCC CATAACCGAT 
ATCAAAGCCA ATGCTGGAGC AGGTTTCCTT TGCTGTCTGA CTGGCTCGAC AAATGCAGAA 
ATGGATGATC CCATGTGGCC CTGTTTCCAT GGCAACAACC TTTACAGTGA CAGCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_695978.4
CDS	550..3309
Translation	MFSVSAAVLRRGVLRYRWDRCCALATVVSGNKTSQQVKQRLKEEVEQMKSQFPGFRPGLVVLQVGDREDSNLYISMKLKAAAEIGINANHIRLPETATENEVLRNIVAVNENPAFHGLIVQLPLDSINPINTEKITNAVAPEKDVDGLTSINAGKLSRGDLGDCFIPCTPNGCMELISQTGVSVMGKNAVVIGRSKIVGAPMHDLLLWSHATVTTCHSKTPDLAEQVGKADILVVGAGRAEMVKGEWVKEGAVVIDCGINHIPDESKASGKRVVGDVHYPSAKQRAGFITPVPGGVGPMTVAMLMKNTVESAKRFLQTHTPGKWNLVYTKKRPHEPQPSDAAISSSCESKPVVQLARELGLFSDEVESYGRSRAKIRLDALKRMEKQPNGKYVVVTGVTSSPLGEGKRTTALGLAQALGAHLNTNAFACVQKSSLETCFGARGPAVGGGYSQIIPLEEVSLQPSGQNELVSAAGRLVMDTVKAYAHYETKLSEKALFDLLVPLREGQRCVSLAQLNRLKKLAIEKSDPLTLTEREIRRFIRLDIDTDSATDSFSLENEMMAVLSLSSSEEEMQHRLANIIVATNKSGDPITTEDLNVSGPLAMLLKEALKPVLMQTVEGTPVFLHTSPLVNVAQGGPSILADKMALKIVGSEGFVVTESGHGADVGLEKFFNIKCLYSGLQPDVLVMVATIRTLKMQGGAPPIQAGLPLPKEYLQENLKQLERGCCHMRRQVEKAQAFGLPVIVAVNKFSSDTDAELELVCGQARQAGAFDAVQCSSWSEGGAGALALAETVQRAAKLPACFKFLYDLQTPASDKLRTVAQKMYGLKDVKLSPKAKEKLALYTKQGFENLPVCIAKTPLSLSNDPMMKGSSTDFMLPITDIKANAGAGFLCCLTGSTNAEMDDPMWPCFHGNNLYSDSK

Target ORF information:

RefSeq Version	XM_690886.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1a, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (mthfd1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_690886.7

ORF Insert Sequence:

ATGTTTTCTG TCAGTGCTGC TGTGCTACGG CGAGGTGTTT TAAGGTACAG ATGGGACAGA 
TGCTGTGCGC TTGCAACAGT TGTTTCTGGA AACAAGACCT CACAGCAGGT GAAACAGCGG 
CTGAAGGAGG AGGTGGAGCA GATGAAGAGT CAGTTTCCAG GTTTCAGGCC TGGTCTTGTG 
GTGCTGCAGG TTGGAGACAG AGAAGACTCA AACCTCTACA TCAGTATGAA GCTCAAAGCT 
GCGGCTGAGA TTGGAATCAA TGCGAACCAC ATCAGGCTTC CTGAAACAGC CACTGAAAAT 
GAAGTCCTAC GCAACATAGT TGCGGTGAAT GAGAATCCTG CATTTCATGG GCTCATTGTG 
CAGCTGCCGC TCGACTCCAT CAACCCAATA AACACTGAGA AAATCACCAA TGCTGTCGCT 
CCAGAGAAAG ATGTTGATGG CTTGACCAGT ATAAATGCGG GGAAGCTGTC TCGTGGAGAC 
CTGGGCGACT GCTTCATCCC ATGCACTCCT AATGGCTGTA TGGAGCTTAT CAGCCAGACT 
GGTGTGAGTG TGATGGGGAA GAATGCTGTC GTGATCGGCC GCAGCAAGAT TGTTGGAGCC 
CCAATGCACG ATCTGCTCTT GTGGAGCCAC GCAACAGTGA CCACCTGCCA CTCGAAGACC 
CCTGACCTCG CCGAACAGGT GGGCAAAGCA GATATTTTGG TTGTGGGTGC TGGCAGAGCA 
GAGATGGTGA AGGGCGAGTG GGTGAAGGAG GGGGCCGTGG TCATTGACTG CGGCATCAAT 
CATATACCAG ATGAGAGTAA AGCTAGCGGG AAGCGGGTGG TGGGTGATGT GCATTACCCC 
TCAGCTAAAC AGCGAGCTGG ATTCATTACC CCAGTGCCTG GTGGAGTCGG ACCCATGACT 
GTGGCTATGC TGATGAAGAA TACAGTTGAA AGTGCGAAGA GGTTTCTGCA AACACACACT 
CCTGGCAAGT GGAACTTAGT GTACACAAAG AAGAGACCCC ATGAGCCTCA GCCCAGCGAT 
GCTGCAATCT CCTCTTCATG TGAAAGCAAA CCAGTAGTTC AGCTCGCCAG AGAATTGGGA 
TTGTTTTCTG ATGAAGTAGA GTCATACGGC AGGAGCAGGG CTAAGATCCG ACTGGATGCT 
TTGAAGCGCA TGGAAAAACA GCCGAATGGG AAATATGTGG TTGTCACCGG AGTTACTTCT 
TCTCCACTGG GTGAGGGAAA GCGCACCACT GCTCTCGGAC TGGCTCAAGC TTTAGGGGCT 
CACCTGAATA CCAACGCTTT CGCTTGTGTT CAAAAGTCCT CACTGGAAAC CTGTTTTGGT 
GCTAGAGGGC CTGCTGTTGG AGGAGGATAC TCTCAAATTA TCCCACTGGA GGAGGTCAGC 
CTTCAGCCTT CAGGCCAGAA TGAGCTCGTC TCTGCTGCCG GTAGACTGGT GATGGACACT 
GTTAAAGCAT ATGCTCACTA TGAGACCAAA CTCTCGGAAA AGGCTTTGTT TGATTTGTTG 
GTTCCTCTCA GAGAGGGTCA AAGGTGCGTC TCCTTGGCTC AGCTTAATCG ACTGAAGAAA 
CTGGCCATTG AGAAATCTGA CCCCTTGACT CTCACAGAGA GAGAGATAAG ACGTTTTATC 
CGATTGGACA TTGACACAGA CTCTGCAACG GATTCATTCT CCTTGGAAAA TGAGATGATG 
GCTGTCCTGT CACTCAGCAG CAGTGAGGAG GAGATGCAGC ACAGACTGGC CAACATCATA 
GTAGCCACAA ACAAATCTGG AGATCCTATC ACTACCGAGG ATTTAAATGT CAGTGGTCCT 
TTGGCTATGT TATTGAAAGA AGCCCTGAAA CCTGTCTTGA TGCAAACTGT CGAGGGTACA 
CCTGTGTTTC TTCACACCAG CCCATTAGTC AATGTTGCCC AAGGCGGTCC TTCCATTCTG 
GCAGACAAAA TGGCTTTAAA AATTGTTGGA TCAGAGGGAT TTGTAGTCAC AGAGTCGGGT 
CATGGAGCTG ATGTTGGCTT GGAGAAGTTT TTCAACATTA AATGCCTCTA CTCAGGTCTT 
CAGCCAGATG TGCTAGTGAT GGTGGCAACC ATTCGTACAT TGAAGATGCA AGGTGGAGCA 
CCTCCTATCC AAGCAGGTTT GCCCCTTCCA AAAGAGTACT TACAAGAGAA CCTCAAGCAG 
CTGGAAAGAG GCTGTTGTCA TATGAGGAGG CAGGTGGAAA AAGCACAAGC ATTCGGCTTG 
CCGGTGATCG TAGCTGTCAA CAAATTCAGC TCTGATACTG ACGCTGAGCT GGAGCTGGTA 
TGTGGCCAGG CGAGGCAGGC TGGTGCCTTT GATGCAGTGC AGTGCAGCAG CTGGTCTGAA 
GGGGGCGCTG GAGCTCTGGC TCTGGCCGAG ACGGTTCAGA GAGCAGCAAA ACTACCTGCC 
TGCTTCAAGT TCCTCTATGA CTTGCAGACT CCAGCCTCTG ATAAGTTAAG GACTGTAGCA 
CAGAAGATGT ACGGTCTGAA AGACGTCAAG CTGTCTCCAA AAGCAAAGGA AAAACTTGCA 
CTTTATACAA AACAGGGCTT TGAAAATTTG CCGGTTTGCA TAGCCAAGAC ACCGCTGTCC 
TTGAGTAATG ACCCCATGAT GAAGGGGTCG TCCACTGACT TCATGCTGCC CATAACCGAT 
ATCAAAGCCA ATGCTGGAGC AGGTTTCCTT TGCTGTCTGA CTGGCTCGAC AAATGCAGAA 
ATGGATGATC CCATGTGGCC CTGTTTCCAT GGCAACAACC TTTACAGTGA CAGCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFD1A ( XM_690886.8 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_695978.4 Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1a, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (mthfd1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa24898
Clone ID Related Accession (Same CDS sequence)	XM_021473374.1 , XM_690886.7 , XM_690886.8
Accession Version	XM_690886.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	C-1-tetrahydrofolate synthase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_690886.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTTCTG TCAGTGCTGC TGTGCTACGG CGAGGTGTTT TAAGGTACAG ATGGGACAGA 
TGCTGTGCGC TTGCAACAGT TGTTTCTGGA AACAAGACCT CACAGCAGGT GAAACAGCGG 
CTGAAGGAGG AGGTGGAGCA GATGAAGAGT CAGTTTCCAG GTTTCAGGCC TGGTCTTGTG 
GTGCTGCAGG TTGGAGACAG AGAAGACTCA AACCTCTACA TCAGTATGAA GCTCAAAGCT 
GCGGCTGAGA TTGGAATCAA TGCGAACCAC ATCAGGCTTC CTGAAACAGC CACTGAAAAT 
GAAGTCCTAC GCAACATAGT TGCGGTGAAT GAGAATCCTG CATTTCATGG GCTCATTGTG 
CAGCTGCCGC TCGACTCCAT CAACCCAATA AACACTGAGA AAATCACCAA TGCTGTCGCT 
CCAGAGAAAG ATGTTGATGG CTTGACCAGT ATAAATGCGG GGAAGCTGTC TCGTGGAGAC 
CTGGGCGACT GCTTCATCCC ATGCACTCCT AATGGCTGTA TGGAGCTTAT CAGCCAGACT 
GGTGTGAGTG TGATGGGGAA GAATGCTGTC GTGATCGGCC GCAGCAAGAT TGTTGGAGCC 
CCAATGCACG ATCTGCTCTT GTGGAGCCAC GCAACAGTGA CCACCTGCCA CTCGAAGACC 
CCTGACCTCG CCGAACAGGT GGGCAAAGCA GATATTTTGG TTGTGGGTGC TGGCAGAGCA 
GAGATGGTGA AGGGCGAGTG GGTGAAGGAG GGGGCCGTGG TCATTGACTG CGGCATCAAT 
CATATACCAG ATGAGAGTAA AGCTAGCGGG AAGCGGGTGG TGGGTGATGT GCATTACCCC 
TCAGCTAAAC AGCGAGCTGG ATTCATTACC CCAGTGCCTG GTGGAGTCGG ACCCATGACT 
GTGGCTATGC TGATGAAGAA TACAGTTGAA AGTGCGAAGA GGTTTCTGCA AACACACACT 
CCTGGCAAGT GGAACTTAGT GTACACAAAG AAGAGACCCC ATGAGCCTCA GCCCAGCGAT 
GCTGCAATCT CCTCTTCATG TGAAAGCAAA CCAGTAGTTC AGCTCGCCAG AGAATTGGGA 
TTGTTTTCTG ATGAAGTAGA GTCATACGGC AGGAGCAGGG CTAAGATCCG ACTGGATGCT 
TTGAAGCGCA TGGAAAAACA GCCGAATGGG AAATATGTGG TTGTCACCGG AGTTACTTCT 
TCTCCACTGG GTGAGGGAAA GCGCACCACT GCTCTCGGAC TGGCTCAAGC TTTAGGGGCT 
CACCTGAATA CCAACGCTTT CGCTTGTGTT CAAAAGTCCT CACTGGAAAC CTGTTTTGGT 
GCTAGAGGGC CTGCTGTTGG AGGAGGATAC TCTCAAATTA TCCCACTGGA GGAGGTCAGC 
CTTCAGCCTT CAGGCCAGAA TGAGCTCGTC TCTGCTGCCG GTAGACTGGT GATGGACACT 
GTTAAAGCAT ATGCTCACTA TGAGACCAAA CTCTCGGAAA AGGCTTTGTT TGATTTGTTG 
GTTCCTCTCA GAGAGGGTCA AAGGTGCGTC TCCTTGGCTC AGCTTAATCG ACTGAAGAAA 
CTGGCCATTG AGAAATCTGA CCCCTTGACT CTCACAGAGA GAGAGATAAG ACGTTTTATC 
CGATTGGACA TTGACACAGA CTCTGCAACG GATTCATTCT CCTTGGAAAA TGAGATGATG 
GCTGTCCTGT CACTCAGCAG CAGTGAGGAG GAGATGCAGC ACAGACTGGC CAACATCATA 
GTAGCCACAA ACAAATCTGG AGATCCTATC ACTACCGAGG ATTTAAATGT CAGTGGTCCT 
TTGGCTATGT TATTGAAAGA AGCCCTGAAA CCTGTCTTGA TGCAAACTGT CGAGGGTACA 
CCTGTGTTTC TTCACACCAG CCCATTAGTC AATGTTGCCC AAGGCGGTCC TTCCATTCTG 
GCAGACAAAA TGGCTTTAAA AATTGTTGGA TCAGAGGGAT TTGTAGTCAC AGAGTCGGGT 
CATGGAGCTG ATGTTGGCTT GGAGAAGTTT TTCAACATTA AATGCCTCTA CTCAGGTCTT 
CAGCCAGATG TGCTAGTGAT GGTGGCAACC ATTCGTACAT TGAAGATGCA AGGTGGAGCA 
CCTCCTATCC AAGCAGGTTT GCCCCTTCCA AAAGAGTACT TACAAGAGAA CCTCAAGCAG 
CTGGAAAGAG GCTGTTGTCA TATGAGGAGG CAGGTGGAAA AAGCACAAGC ATTCGGCTTG 
CCGGTGATCG TAGCTGTCAA CAAATTCAGC TCTGATACTG ACGCTGAGCT GGAGCTGGTA 
TGTGGCCAGG CGAGGCAGGC TGGTGCCTTT GATGCAGTGC AGTGCAGCAG CTGGTCTGAA 
GGGGGCGCTG GAGCTCTGGC TCTGGCCGAG ACGGTTCAGA GAGCAGCAAA ACTACCTGCC 
TGCTTCAAGT TCCTCTATGA CTTGCAGACT CCAGCCTCTG ATAAGTTAAG GACTGTAGCA 
CAGAAGATGT ACGGTCTGAA AGACGTCAAG CTGTCTCCAA AAGCAAAGGA AAAACTTGCA 
CTTTATACAA AACAGGGCTT TGAAAATTTG CCGGTTTGCA TAGCCAAGAC ACCGCTGTCC 
TTGAGTAATG ACCCCATGAT GAAGGGGTCG TCCACTGACT TCATGCTGCC CATAACCGAT 
ATCAAAGCCA ATGCTGGAGC AGGTTTCCTT TGCTGTCTGA CTGGCTCGAC AAATGCAGAA 
ATGGATGATC CCATGTGGCC CTGTTTCCAT GGCAACAACC TTTACAGTGA CAGCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_695978.4
CDS	733..3492
Translation	MFSVSAAVLRRGVLRYRWDRCCALATVVSGNKTSQQVKQRLKEEVEQMKSQFPGFRPGLVVLQVGDREDSNLYISMKLKAAAEIGINANHIRLPETATENEVLRNIVAVNENPAFHGLIVQLPLDSINPINTEKITNAVAPEKDVDGLTSINAGKLSRGDLGDCFIPCTPNGCMELISQTGVSVMGKNAVVIGRSKIVGAPMHDLLLWSHATVTTCHSKTPDLAEQVGKADILVVGAGRAEMVKGEWVKEGAVVIDCGINHIPDESKASGKRVVGDVHYPSAKQRAGFITPVPGGVGPMTVAMLMKNTVESAKRFLQTHTPGKWNLVYTKKRPHEPQPSDAAISSSCESKPVVQLARELGLFSDEVESYGRSRAKIRLDALKRMEKQPNGKYVVVTGVTSSPLGEGKRTTALGLAQALGAHLNTNAFACVQKSSLETCFGARGPAVGGGYSQIIPLEEVSLQPSGQNELVSAAGRLVMDTVKAYAHYETKLSEKALFDLLVPLREGQRCVSLAQLNRLKKLAIEKSDPLTLTEREIRRFIRLDIDTDSATDSFSLENEMMAVLSLSSSEEEMQHRLANIIVATNKSGDPITTEDLNVSGPLAMLLKEALKPVLMQTVEGTPVFLHTSPLVNVAQGGPSILADKMALKIVGSEGFVVTESGHGADVGLEKFFNIKCLYSGLQPDVLVMVATIRTLKMQGGAPPIQAGLPLPKEYLQENLKQLERGCCHMRRQVEKAQAFGLPVIVAVNKFSSDTDAELELVCGQARQAGAFDAVQCSSWSEGGAGALALAETVQRAAKLPACFKFLYDLQTPASDKLRTVAQKMYGLKDVKLSPKAKEKLALYTKQGFENLPVCIAKTPLSLSNDPMMKGSSTDFMLPITDIKANAGAGFLCCLTGSTNAEMDDPMWPCFHGNNLYSDSK

Target ORF information:

RefSeq Version	XM_690886.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1a, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (mthfd1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_690886.8

ORF Insert Sequence:

ATGTTTTCTG TCAGTGCTGC TGTGCTACGG CGAGGTGTTT TAAGGTACAG ATGGGACAGA 
TGCTGTGCGC TTGCAACAGT TGTTTCTGGA AACAAGACCT CACAGCAGGT GAAACAGCGG 
CTGAAGGAGG AGGTGGAGCA GATGAAGAGT CAGTTTCCAG GTTTCAGGCC TGGTCTTGTG 
GTGCTGCAGG TTGGAGACAG AGAAGACTCA AACCTCTACA TCAGTATGAA GCTCAAAGCT 
GCGGCTGAGA TTGGAATCAA TGCGAACCAC ATCAGGCTTC CTGAAACAGC CACTGAAAAT 
GAAGTCCTAC GCAACATAGT TGCGGTGAAT GAGAATCCTG CATTTCATGG GCTCATTGTG 
CAGCTGCCGC TCGACTCCAT CAACCCAATA AACACTGAGA AAATCACCAA TGCTGTCGCT 
CCAGAGAAAG ATGTTGATGG CTTGACCAGT ATAAATGCGG GGAAGCTGTC TCGTGGAGAC 
CTGGGCGACT GCTTCATCCC ATGCACTCCT AATGGCTGTA TGGAGCTTAT CAGCCAGACT 
GGTGTGAGTG TGATGGGGAA GAATGCTGTC GTGATCGGCC GCAGCAAGAT TGTTGGAGCC 
CCAATGCACG ATCTGCTCTT GTGGAGCCAC GCAACAGTGA CCACCTGCCA CTCGAAGACC 
CCTGACCTCG CCGAACAGGT GGGCAAAGCA GATATTTTGG TTGTGGGTGC TGGCAGAGCA 
GAGATGGTGA AGGGCGAGTG GGTGAAGGAG GGGGCCGTGG TCATTGACTG CGGCATCAAT 
CATATACCAG ATGAGAGTAA AGCTAGCGGG AAGCGGGTGG TGGGTGATGT GCATTACCCC 
TCAGCTAAAC AGCGAGCTGG ATTCATTACC CCAGTGCCTG GTGGAGTCGG ACCCATGACT 
GTGGCTATGC TGATGAAGAA TACAGTTGAA AGTGCGAAGA GGTTTCTGCA AACACACACT 
CCTGGCAAGT GGAACTTAGT GTACACAAAG AAGAGACCCC ATGAGCCTCA GCCCAGCGAT 
GCTGCAATCT CCTCTTCATG TGAAAGCAAA CCAGTAGTTC AGCTCGCCAG AGAATTGGGA 
TTGTTTTCTG ATGAAGTAGA GTCATACGGC AGGAGCAGGG CTAAGATCCG ACTGGATGCT 
TTGAAGCGCA TGGAAAAACA GCCGAATGGG AAATATGTGG TTGTCACCGG AGTTACTTCT 
TCTCCACTGG GTGAGGGAAA GCGCACCACT GCTCTCGGAC TGGCTCAAGC TTTAGGGGCT 
CACCTGAATA CCAACGCTTT CGCTTGTGTT CAAAAGTCCT CACTGGAAAC CTGTTTTGGT 
GCTAGAGGGC CTGCTGTTGG AGGAGGATAC TCTCAAATTA TCCCACTGGA GGAGGTCAGC 
CTTCAGCCTT CAGGCCAGAA TGAGCTCGTC TCTGCTGCCG GTAGACTGGT GATGGACACT 
GTTAAAGCAT ATGCTCACTA TGAGACCAAA CTCTCGGAAA AGGCTTTGTT TGATTTGTTG 
GTTCCTCTCA GAGAGGGTCA AAGGTGCGTC TCCTTGGCTC AGCTTAATCG ACTGAAGAAA 
CTGGCCATTG AGAAATCTGA CCCCTTGACT CTCACAGAGA GAGAGATAAG ACGTTTTATC 
CGATTGGACA TTGACACAGA CTCTGCAACG GATTCATTCT CCTTGGAAAA TGAGATGATG 
GCTGTCCTGT CACTCAGCAG CAGTGAGGAG GAGATGCAGC ACAGACTGGC CAACATCATA 
GTAGCCACAA ACAAATCTGG AGATCCTATC ACTACCGAGG ATTTAAATGT CAGTGGTCCT 
TTGGCTATGT TATTGAAAGA AGCCCTGAAA CCTGTCTTGA TGCAAACTGT CGAGGGTACA 
CCTGTGTTTC TTCACACCAG CCCATTAGTC AATGTTGCCC AAGGCGGTCC TTCCATTCTG 
GCAGACAAAA TGGCTTTAAA AATTGTTGGA TCAGAGGGAT TTGTAGTCAC AGAGTCGGGT 
CATGGAGCTG ATGTTGGCTT GGAGAAGTTT TTCAACATTA AATGCCTCTA CTCAGGTCTT 
CAGCCAGATG TGCTAGTGAT GGTGGCAACC ATTCGTACAT TGAAGATGCA AGGTGGAGCA 
CCTCCTATCC AAGCAGGTTT GCCCCTTCCA AAAGAGTACT TACAAGAGAA CCTCAAGCAG 
CTGGAAAGAG GCTGTTGTCA TATGAGGAGG CAGGTGGAAA AAGCACAAGC ATTCGGCTTG 
CCGGTGATCG TAGCTGTCAA CAAATTCAGC TCTGATACTG ACGCTGAGCT GGAGCTGGTA 
TGTGGCCAGG CGAGGCAGGC TGGTGCCTTT GATGCAGTGC AGTGCAGCAG CTGGTCTGAA 
GGGGGCGCTG GAGCTCTGGC TCTGGCCGAG ACGGTTCAGA GAGCAGCAAA ACTACCTGCC 
TGCTTCAAGT TCCTCTATGA CTTGCAGACT CCAGCCTCTG ATAAGTTAAG GACTGTAGCA 
CAGAAGATGT ACGGTCTGAA AGACGTCAAG CTGTCTCCAA AAGCAAAGGA AAAACTTGCA 
CTTTATACAA AACAGGGCTT TGAAAATTTG CCGGTTTGCA TAGCCAAGAC ACCGCTGTCC 
TTGAGTAATG ACCCCATGAT GAAGGGGTCG TCCACTGACT TCATGCTGCC CATAACCGAT 
ATCAAAGCCA ATGCTGGAGC AGGTTTCCTT TGCTGTCTGA CTGGCTCGAC AAATGCAGAA 
ATGGATGATC CCATGTGGCC CTGTTTCCAT GGCAACAACC TTTACAGTGA CAGCAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFD1A ( XM_017352710.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017208199.1 Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1a, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (mthfd1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa56730
Clone ID Related Accession (Same CDS sequence)	XM_017352710.2 , XM_021473375.1 , XM_017352710.1
Accession Version	XM_017352710.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	C-1-tetrahydrofolate synthase, cytoplasmic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352710.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACAGA TGCTGTGCGC TTGCAACAGT TGTTTCTGGA AACAAGACCT CACAGCAGGT 
GAAACAGCGG CTGAAGGAGG AGGTGGAGCA GATGAAGAGT CAGTTTCCAG GTTTCAGGCC 
TGGTCTTGTG GTGCTGCAGG TGGTCATTTG GTTGGAGACA GAGAAGACTC AAACCTCTAC 
ATCAGTATGA AGCTCAAAGC TGCGGCTGAG ATTGGAATCA ATGCGAACCA CATCAGGCTT 
CCTGAAACAG CCACTGAAAA TGAAGTCCTA CGCAACATAG TTGCGGTGAA TGAGAATCCT 
GCATTTCATG GGCTCATTGT GCAGCTGCCG CTCGACTCCA TCAACCCAAT AAACACTGAG 
AAAATCACCA ATGCTGTCGC TCCAGAGAAA GATGTTGATG GCTTGACCAG TATAAATGCG 
GGGAAGCTGT CTCGTGGAGA CCTGGGCGAC TGCTTCATCC CATGCACTCC TAATGGCTGT 
ATGGAGCTTA TCAGCCAGAC TGGTGTGAGT GTGATGGGGA AGAATGCTGT CGTGATCGGC 
CGCAGCAAGA TTGTTGGAGC CCCAATGCAC GATCTGCTCT TGTGGAGCCA CGCAACAGTG 
ACCACCTGCC ACTCGAAGAC CCCTGACCTC GCCGAACAGG TGGGCAAAGC AGATATTTTG 
GTTGTGGGTG CTGGCAGAGC AGAGATGGTG AAGGGCGAGT GGGTGAAGGA GGGGGCCGTG 
GTCATTGACT GCGGCATCAA TCATATACCA GATGAGAGTA AAGCTAGCGG GAAGCGGGTG 
GTGGGTGATG TGCATTACCC CTCAGCTAAA CAGCGAGCTG GATTCATTAC CCCAGTGCCT 
GGTGGAGTCG GACCCATGAC TGTGGCTATG CTGATGAAGA ATACAGTTGA AAGTGCGAAG 
AGGTTTCTGC AAACACACAC TCCTGGCAAG TGGAACTTAG TGTACACAAA GAAGAGACCC 
CATGAGCCTC AGCCCAGCGA TGCTGCAATC TCCTCTTCAT GTGAAAGCAA ACCAGTAGTT 
CAGCTCGCCA GAGAATTGGG ATTGTTTTCT GATGAAGTAG AGTCATACGG CAGGAGCAGG 
GCTAAGATCC GACTGGATGC TTTGAAGCGC ATGGAAAAAC AGCCGAATGG GAAATATGTG 
GTTGTCACCG GAGTTACTTC TTCTCCACTG GGTGAGGGAA AGCGCACCAC TGCTCTCGGA 
CTGGCTCAAG CTTTAGGGGC TCACCTGAAT ACCAACGCTT TCGCTTGTGT TCAAAAGTCC 
TCACTGGAAA CCTGTTTTGG TGCTAGAGGG CCTGCTGTTG GAGGAGGATA CTCTCAAATT 
ATCCCACTGG AGGAGGTCAG CCTTCAGCCT TCAGGCCAGA ATGAGCTCGT CTCTGCTGCC 
GGTAGACTGG TGATGGACAC TGTTAAAGCA TATGCTCACT ATGAGACCAA ACTCTCGGAA 
AAGGCTTTGT TTGATTTGTT GGTTCCTCTC AGAGAGGGTC AAAGGTGCGT CTCCTTGGCT 
CAGCTTAATC GACTGAAGAA ACTGGCCATT GAGAAATCTG ACCCCTTGAC TCTCACAGAG 
AGAGAGATAA GACGTTTTAT CCGATTGGAC ATTGACACAG ACTCTGCAAC GGATTCATTC 
TCCTTGGAAA ATGAGATGAT GGCTGTCCTG TCACTCAGCA GCAGTGAGGA GGAGATGCAG 
CACAGACTGG CCAACATCAT AGTAGCCACA AACAAATCTG GAGATCCTAT CACTACCGAG 
GATTTAAATG TCAGTGGTCC TTTGGCTATG TTATTGAAAG AAGCCCTGAA ACCTGTCTTG 
ATGCAAACTG TCGAGGGTAC ACCTGTGTTT CTTCACACCA GCCCATTAGT CAATGTTGCC 
CAAGGCGGTC CTTCCATTCT GGCAGACAAA ATGGCTTTAA AAATTGTTGG ATCAGAGGGA 
TTTGTAGTCA CAGAGTCGGG TCATGGAGCT GATGTTGGCT TGGAGAAGTT TTTCAACATT 
AAATGCCTCT ACTCAGGTCT TCAGCCAGAT GTGCTAGTGA TGGTGGCAAC CATTCGTACA 
TTGAAGATGC AAGGTGGAGC ACCTCCTATC CAAGCAGGTT TGCCCCTTCC AAAAGAGTAC 
TTACAAGAGA ACCTCAAGCA GCTGGAAAGA GGCTGTTGTC ATATGAGGAG GCAGGTGGAA 
AAAGCACAAG CATTCGGCTT GCCGGTGATC GTAGCTGTCA ACAAATTCAG CTCTGATACT 
GACGCTGAGC TGGAGCTGGT ATGTGGCCAG GCGAGGCAGG CTGGTGCCTT TGATGCAGTG 
CAGTGCAGCA GCTGGTCTGA AGGGGGCGCT GGAGCTCTGG CTCTGGCCGA GACGGTTCAG 
AGAGCAGCAA AACTACCTGC CTGCTTCAAG TTCCTCTATG ACTTGCAGAC TCCAGCCTCT 
GATAAGTTAA GGACTGTAGC ACAGAAGATG TACGGTCTGA AAGACGTCAA GCTGTCTCCA 
AAAGCAAAGG AAAAACTTGC ACTTTATACA AAACAGGGCT TTGAAAATTT GCCGGTTTGC 
ATAGCCAAGA CACCGCTGTC CTTGAGTAAT GACCCCATGA TGAAGGGGTC GTCCACTGAC 
TTCATGCTGC CCATAACCGA TATCAAAGCC AATGCTGGAG CAGGTTTCCT TTGCTGTCTG 
ACTGGCTCGA CAAATGCAGA AATGGATGAT CCCATGTGGC CCTGTTTCCA TGGCAACAAC 
CTTTACAGTG ACAGCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208199.1
CDS	783..3503
Translation	MGQMLCACNSCFWKQDLTAGETAAEGGGGADEESVSRFQAWSCGAAGGHLVGDREDSNLYISMKLKAAAEIGINANHIRLPETATENEVLRNIVAVNENPAFHGLIVQLPLDSINPINTEKITNAVAPEKDVDGLTSINAGKLSRGDLGDCFIPCTPNGCMELISQTGVSVMGKNAVVIGRSKIVGAPMHDLLLWSHATVTTCHSKTPDLAEQVGKADILVVGAGRAEMVKGEWVKEGAVVIDCGINHIPDESKASGKRVVGDVHYPSAKQRAGFITPVPGGVGPMTVAMLMKNTVESAKRFLQTHTPGKWNLVYTKKRPHEPQPSDAAISSSCESKPVVQLARELGLFSDEVESYGRSRAKIRLDALKRMEKQPNGKYVVVTGVTSSPLGEGKRTTALGLAQALGAHLNTNAFACVQKSSLETCFGARGPAVGGGYSQIIPLEEVSLQPSGQNELVSAAGRLVMDTVKAYAHYETKLSEKALFDLLVPLREGQRCVSLAQLNRLKKLAIEKSDPLTLTEREIRRFIRLDIDTDSATDSFSLENEMMAVLSLSSSEEEMQHRLANIIVATNKSGDPITTEDLNVSGPLAMLLKEALKPVLMQTVEGTPVFLHTSPLVNVAQGGPSILADKMALKIVGSEGFVVTESGHGADVGLEKFFNIKCLYSGLQPDVLVMVATIRTLKMQGGAPPIQAGLPLPKEYLQENLKQLERGCCHMRRQVEKAQAFGLPVIVAVNKFSSDTDAELELVCGQARQAGAFDAVQCSSWSEGGAGALALAETVQRAAKLPACFKFLYDLQTPASDKLRTVAQKMYGLKDVKLSPKAKEKLALYTKQGFENLPVCIAKTPLSLSNDPMMKGSSTDFMLPITDIKANAGAGFLCCLTGSTNAEMDDPMWPCFHGNNLYSDSK

Target ORF information:

RefSeq Version	XM_017352710.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1a, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (mthfd1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352710.2

ORF Insert Sequence:

ATGGGACAGA TGCTGTGCGC TTGCAACAGT TGTTTCTGGA AACAAGACCT CACAGCAGGT 
GAAACAGCGG CTGAAGGAGG AGGTGGAGCA GATGAAGAGT CAGTTTCCAG GTTTCAGGCC 
TGGTCTTGTG GTGCTGCAGG TGGTCATTTG GTTGGAGACA GAGAAGACTC AAACCTCTAC 
ATCAGTATGA AGCTCAAAGC TGCGGCTGAG ATTGGAATCA ATGCGAACCA CATCAGGCTT 
CCTGAAACAG CCACTGAAAA TGAAGTCCTA CGCAACATAG TTGCGGTGAA TGAGAATCCT 
GCATTTCATG GGCTCATTGT GCAGCTGCCG CTCGACTCCA TCAACCCAAT AAACACTGAG 
AAAATCACCA ATGCTGTCGC TCCAGAGAAA GATGTTGATG GCTTGACCAG TATAAATGCG 
GGGAAGCTGT CTCGTGGAGA CCTGGGCGAC TGCTTCATCC CATGCACTCC TAATGGCTGT 
ATGGAGCTTA TCAGCCAGAC TGGTGTGAGT GTGATGGGGA AGAATGCTGT CGTGATCGGC 
CGCAGCAAGA TTGTTGGAGC CCCAATGCAC GATCTGCTCT TGTGGAGCCA CGCAACAGTG 
ACCACCTGCC ACTCGAAGAC CCCTGACCTC GCCGAACAGG TGGGCAAAGC AGATATTTTG 
GTTGTGGGTG CTGGCAGAGC AGAGATGGTG AAGGGCGAGT GGGTGAAGGA GGGGGCCGTG 
GTCATTGACT GCGGCATCAA TCATATACCA GATGAGAGTA AAGCTAGCGG GAAGCGGGTG 
GTGGGTGATG TGCATTACCC CTCAGCTAAA CAGCGAGCTG GATTCATTAC CCCAGTGCCT 
GGTGGAGTCG GACCCATGAC TGTGGCTATG CTGATGAAGA ATACAGTTGA AAGTGCGAAG 
AGGTTTCTGC AAACACACAC TCCTGGCAAG TGGAACTTAG TGTACACAAA GAAGAGACCC 
CATGAGCCTC AGCCCAGCGA TGCTGCAATC TCCTCTTCAT GTGAAAGCAA ACCAGTAGTT 
CAGCTCGCCA GAGAATTGGG ATTGTTTTCT GATGAAGTAG AGTCATACGG CAGGAGCAGG 
GCTAAGATCC GACTGGATGC TTTGAAGCGC ATGGAAAAAC AGCCGAATGG GAAATATGTG 
GTTGTCACCG GAGTTACTTC TTCTCCACTG GGTGAGGGAA AGCGCACCAC TGCTCTCGGA 
CTGGCTCAAG CTTTAGGGGC TCACCTGAAT ACCAACGCTT TCGCTTGTGT TCAAAAGTCC 
TCACTGGAAA CCTGTTTTGG TGCTAGAGGG CCTGCTGTTG GAGGAGGATA CTCTCAAATT 
ATCCCACTGG AGGAGGTCAG CCTTCAGCCT TCAGGCCAGA ATGAGCTCGT CTCTGCTGCC 
GGTAGACTGG TGATGGACAC TGTTAAAGCA TATGCTCACT ATGAGACCAA ACTCTCGGAA 
AAGGCTTTGT TTGATTTGTT GGTTCCTCTC AGAGAGGGTC AAAGGTGCGT CTCCTTGGCT 
CAGCTTAATC GACTGAAGAA ACTGGCCATT GAGAAATCTG ACCCCTTGAC TCTCACAGAG 
AGAGAGATAA GACGTTTTAT CCGATTGGAC ATTGACACAG ACTCTGCAAC GGATTCATTC 
TCCTTGGAAA ATGAGATGAT GGCTGTCCTG TCACTCAGCA GCAGTGAGGA GGAGATGCAG 
CACAGACTGG CCAACATCAT AGTAGCCACA AACAAATCTG GAGATCCTAT CACTACCGAG 
GATTTAAATG TCAGTGGTCC TTTGGCTATG TTATTGAAAG AAGCCCTGAA ACCTGTCTTG 
ATGCAAACTG TCGAGGGTAC ACCTGTGTTT CTTCACACCA GCCCATTAGT CAATGTTGCC 
CAAGGCGGTC CTTCCATTCT GGCAGACAAA ATGGCTTTAA AAATTGTTGG ATCAGAGGGA 
TTTGTAGTCA CAGAGTCGGG TCATGGAGCT GATGTTGGCT TGGAGAAGTT TTTCAACATT 
AAATGCCTCT ACTCAGGTCT TCAGCCAGAT GTGCTAGTGA TGGTGGCAAC CATTCGTACA 
TTGAAGATGC AAGGTGGAGC ACCTCCTATC CAAGCAGGTT TGCCCCTTCC AAAAGAGTAC 
TTACAAGAGA ACCTCAAGCA GCTGGAAAGA GGCTGTTGTC ATATGAGGAG GCAGGTGGAA 
AAAGCACAAG CATTCGGCTT GCCGGTGATC GTAGCTGTCA ACAAATTCAG CTCTGATACT 
GACGCTGAGC TGGAGCTGGT ATGTGGCCAG GCGAGGCAGG CTGGTGCCTT TGATGCAGTG 
CAGTGCAGCA GCTGGTCTGA AGGGGGCGCT GGAGCTCTGG CTCTGGCCGA GACGGTTCAG 
AGAGCAGCAA AACTACCTGC CTGCTTCAAG TTCCTCTATG ACTTGCAGAC TCCAGCCTCT 
GATAAGTTAA GGACTGTAGC ACAGAAGATG TACGGTCTGA AAGACGTCAA GCTGTCTCCA 
AAAGCAAAGG AAAAACTTGC ACTTTATACA AAACAGGGCT TTGAAAATTT GCCGGTTTGC 
ATAGCCAAGA CACCGCTGTC CTTGAGTAAT GACCCCATGA TGAAGGGGTC GTCCACTGAC 
TTCATGCTGC CCATAACCGA TATCAAAGCC AATGCTGGAG CAGGTTTCCT TTGCTGTCTG 
ACTGGCTCGA CAAATGCAGA AATGGATGAT CCCATGTGGC CCTGTTTCCA TGGCAACAAC 
CTTTACAGTG ACAGCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFD1A ( XM_021473375.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021329050.1 Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1a, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (mthfd1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa56730
Clone ID Related Accession (Same CDS sequence)	XM_017352710.2 , XM_021473375.1 , XM_017352710.1
Accession Version	XM_021473375.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	C-1-tetrahydrofolate synthase, cytoplasmic isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395075.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACAGA TGCTGTGCGC TTGCAACAGT TGTTTCTGGA AACAAGACCT CACAGCAGGT 
GAAACAGCGG CTGAAGGAGG AGGTGGAGCA GATGAAGAGT CAGTTTCCAG GTTTCAGGCC 
TGGTCTTGTG GTGCTGCAGG TGGTCATTTG GTTGGAGACA GAGAAGACTC AAACCTCTAC 
ATCAGTATGA AGCTCAAAGC TGCGGCTGAG ATTGGAATCA ATGCGAACCA CATCAGGCTT 
CCTGAAACAG CCACTGAAAA TGAAGTCCTA CGCAACATAG TTGCGGTGAA TGAGAATCCT 
GCATTTCATG GGCTCATTGT GCAGCTGCCG CTCGACTCCA TCAACCCAAT AAACACTGAG 
AAAATCACCA ATGCTGTCGC TCCAGAGAAA GATGTTGATG GCTTGACCAG TATAAATGCG 
GGGAAGCTGT CTCGTGGAGA CCTGGGCGAC TGCTTCATCC CATGCACTCC TAATGGCTGT 
ATGGAGCTTA TCAGCCAGAC TGGTGTGAGT GTGATGGGGA AGAATGCTGT CGTGATCGGC 
CGCAGCAAGA TTGTTGGAGC CCCAATGCAC GATCTGCTCT TGTGGAGCCA CGCAACAGTG 
ACCACCTGCC ACTCGAAGAC CCCTGACCTC GCCGAACAGG TGGGCAAAGC AGATATTTTG 
GTTGTGGGTG CTGGCAGAGC AGAGATGGTG AAGGGCGAGT GGGTGAAGGA GGGGGCCGTG 
GTCATTGACT GCGGCATCAA TCATATACCA GATGAGAGTA AAGCTAGCGG GAAGCGGGTG 
GTGGGTGATG TGCATTACCC CTCAGCTAAA CAGCGAGCTG GATTCATTAC CCCAGTGCCT 
GGTGGAGTCG GACCCATGAC TGTGGCTATG CTGATGAAGA ATACAGTTGA AAGTGCGAAG 
AGGTTTCTGC AAACACACAC TCCTGGCAAG TGGAACTTAG TGTACACAAA GAAGAGACCC 
CATGAGCCTC AGCCCAGCGA TGCTGCAATC TCCTCTTCAT GTGAAAGCAA ACCAGTAGTT 
CAGCTCGCCA GAGAATTGGG ATTGTTTTCT GATGAAGTAG AGTCATACGG CAGGAGCAGG 
GCTAAGATCC GACTGGATGC TTTGAAGCGC ATGGAAAAAC AGCCGAATGG GAAATATGTG 
GTTGTCACCG GAGTTACTTC TTCTCCACTG GGTGAGGGAA AGCGCACCAC TGCTCTCGGA 
CTGGCTCAAG CTTTAGGGGC TCACCTGAAT ACCAACGCTT TCGCTTGTGT TCAAAAGTCC 
TCACTGGAAA CCTGTTTTGG TGCTAGAGGG CCTGCTGTTG GAGGAGGATA CTCTCAAATT 
ATCCCACTGG AGGAGGTCAG CCTTCAGCCT TCAGGCCAGA ATGAGCTCGT CTCTGCTGCC 
GGTAGACTGG TGATGGACAC TGTTAAAGCA TATGCTCACT ATGAGACCAA ACTCTCGGAA 
AAGGCTTTGT TTGATTTGTT GGTTCCTCTC AGAGAGGGTC AAAGGTGCGT CTCCTTGGCT 
CAGCTTAATC GACTGAAGAA ACTGGCCATT GAGAAATCTG ACCCCTTGAC TCTCACAGAG 
AGAGAGATAA GACGTTTTAT CCGATTGGAC ATTGACACAG ACTCTGCAAC GGATTCATTC 
TCCTTGGAAA ATGAGATGAT GGCTGTCCTG TCACTCAGCA GCAGTGAGGA GGAGATGCAG 
CACAGACTGG CCAACATCAT AGTAGCCACA AACAAATCTG GAGATCCTAT CACTACCGAG 
GATTTAAATG TCAGTGGTCC TTTGGCTATG TTATTGAAAG AAGCCCTGAA ACCTGTCTTG 
ATGCAAACTG TCGAGGGTAC ACCTGTGTTT CTTCACACCA GCCCATTAGT CAATGTTGCC 
CAAGGCGGTC CTTCCATTCT GGCAGACAAA ATGGCTTTAA AAATTGTTGG ATCAGAGGGA 
TTTGTAGTCA CAGAGTCGGG TCATGGAGCT GATGTTGGCT TGGAGAAGTT TTTCAACATT 
AAATGCCTCT ACTCAGGTCT TCAGCCAGAT GTGCTAGTGA TGGTGGCAAC CATTCGTACA 
TTGAAGATGC AAGGTGGAGC ACCTCCTATC CAAGCAGGTT TGCCCCTTCC AAAAGAGTAC 
TTACAAGAGA ACCTCAAGCA GCTGGAAAGA GGCTGTTGTC ATATGAGGAG GCAGGTGGAA 
AAAGCACAAG CATTCGGCTT GCCGGTGATC GTAGCTGTCA ACAAATTCAG CTCTGATACT 
GACGCTGAGC TGGAGCTGGT ATGTGGCCAG GCGAGGCAGG CTGGTGCCTT TGATGCAGTG 
CAGTGCAGCA GCTGGTCTGA AGGGGGCGCT GGAGCTCTGG CTCTGGCCGA GACGGTTCAG 
AGAGCAGCAA AACTACCTGC CTGCTTCAAG TTCCTCTATG ACTTGCAGAC TCCAGCCTCT 
GATAAGTTAA GGACTGTAGC ACAGAAGATG TACGGTCTGA AAGACGTCAA GCTGTCTCCA 
AAAGCAAAGG AAAAACTTGC ACTTTATACA AAACAGGGCT TTGAAAATTT GCCGGTTTGC 
ATAGCCAAGA CACCGCTGTC CTTGAGTAAT GACCCCATGA TGAAGGGGTC GTCCACTGAC 
TTCATGCTGC CCATAACCGA TATCAAAGCC AATGCTGGAG CAGGTTTCCT TTGCTGTCTG 
ACTGGCTCGA CAAATGCAGA AATGGATGAT CCCATGTGGC CCTGTTTCCA TGGCAACAAC 
CTTTACAGTG ACAGCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329050.1
CDS	783..3503
Translation	MGQMLCACNSCFWKQDLTAGETAAEGGGGADEESVSRFQAWSCGAAGGHLVGDREDSNLYISMKLKAAAEIGINANHIRLPETATENEVLRNIVAVNENPAFHGLIVQLPLDSINPINTEKITNAVAPEKDVDGLTSINAGKLSRGDLGDCFIPCTPNGCMELISQTGVSVMGKNAVVIGRSKIVGAPMHDLLLWSHATVTTCHSKTPDLAEQVGKADILVVGAGRAEMVKGEWVKEGAVVIDCGINHIPDESKASGKRVVGDVHYPSAKQRAGFITPVPGGVGPMTVAMLMKNTVESAKRFLQTHTPGKWNLVYTKKRPHEPQPSDAAISSSCESKPVVQLARELGLFSDEVESYGRSRAKIRLDALKRMEKQPNGKYVVVTGVTSSPLGEGKRTTALGLAQALGAHLNTNAFACVQKSSLETCFGARGPAVGGGYSQIIPLEEVSLQPSGQNELVSAAGRLVMDTVKAYAHYETKLSEKALFDLLVPLREGQRCVSLAQLNRLKKLAIEKSDPLTLTEREIRRFIRLDIDTDSATDSFSLENEMMAVLSLSSSEEEMQHRLANIIVATNKSGDPITTEDLNVSGPLAMLLKEALKPVLMQTVEGTPVFLHTSPLVNVAQGGPSILADKMALKIVGSEGFVVTESGHGADVGLEKFFNIKCLYSGLQPDVLVMVATIRTLKMQGGAPPIQAGLPLPKEYLQENLKQLERGCCHMRRQVEKAQAFGLPVIVAVNKFSSDTDAELELVCGQARQAGAFDAVQCSSWSEGGAGALALAETVQRAAKLPACFKFLYDLQTPASDKLRTVAQKMYGLKDVKLSPKAKEKLALYTKQGFENLPVCIAKTPLSLSNDPMMKGSSTDFMLPITDIKANAGAGFLCCLTGSTNAEMDDPMWPCFHGNNLYSDSK

Target ORF information:

RefSeq Version	XM_021473375.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1a, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (mthfd1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473375.1

ORF Insert Sequence:

ATGGGACAGA TGCTGTGCGC TTGCAACAGT TGTTTCTGGA AACAAGACCT CACAGCAGGT 
GAAACAGCGG CTGAAGGAGG AGGTGGAGCA GATGAAGAGT CAGTTTCCAG GTTTCAGGCC 
TGGTCTTGTG GTGCTGCAGG TGGTCATTTG GTTGGAGACA GAGAAGACTC AAACCTCTAC 
ATCAGTATGA AGCTCAAAGC TGCGGCTGAG ATTGGAATCA ATGCGAACCA CATCAGGCTT 
CCTGAAACAG CCACTGAAAA TGAAGTCCTA CGCAACATAG TTGCGGTGAA TGAGAATCCT 
GCATTTCATG GGCTCATTGT GCAGCTGCCG CTCGACTCCA TCAACCCAAT AAACACTGAG 
AAAATCACCA ATGCTGTCGC TCCAGAGAAA GATGTTGATG GCTTGACCAG TATAAATGCG 
GGGAAGCTGT CTCGTGGAGA CCTGGGCGAC TGCTTCATCC CATGCACTCC TAATGGCTGT 
ATGGAGCTTA TCAGCCAGAC TGGTGTGAGT GTGATGGGGA AGAATGCTGT CGTGATCGGC 
CGCAGCAAGA TTGTTGGAGC CCCAATGCAC GATCTGCTCT TGTGGAGCCA CGCAACAGTG 
ACCACCTGCC ACTCGAAGAC CCCTGACCTC GCCGAACAGG TGGGCAAAGC AGATATTTTG 
GTTGTGGGTG CTGGCAGAGC AGAGATGGTG AAGGGCGAGT GGGTGAAGGA GGGGGCCGTG 
GTCATTGACT GCGGCATCAA TCATATACCA GATGAGAGTA AAGCTAGCGG GAAGCGGGTG 
GTGGGTGATG TGCATTACCC CTCAGCTAAA CAGCGAGCTG GATTCATTAC CCCAGTGCCT 
GGTGGAGTCG GACCCATGAC TGTGGCTATG CTGATGAAGA ATACAGTTGA AAGTGCGAAG 
AGGTTTCTGC AAACACACAC TCCTGGCAAG TGGAACTTAG TGTACACAAA GAAGAGACCC 
CATGAGCCTC AGCCCAGCGA TGCTGCAATC TCCTCTTCAT GTGAAAGCAA ACCAGTAGTT 
CAGCTCGCCA GAGAATTGGG ATTGTTTTCT GATGAAGTAG AGTCATACGG CAGGAGCAGG 
GCTAAGATCC GACTGGATGC TTTGAAGCGC ATGGAAAAAC AGCCGAATGG GAAATATGTG 
GTTGTCACCG GAGTTACTTC TTCTCCACTG GGTGAGGGAA AGCGCACCAC TGCTCTCGGA 
CTGGCTCAAG CTTTAGGGGC TCACCTGAAT ACCAACGCTT TCGCTTGTGT TCAAAAGTCC 
TCACTGGAAA CCTGTTTTGG TGCTAGAGGG CCTGCTGTTG GAGGAGGATA CTCTCAAATT 
ATCCCACTGG AGGAGGTCAG CCTTCAGCCT TCAGGCCAGA ATGAGCTCGT CTCTGCTGCC 
GGTAGACTGG TGATGGACAC TGTTAAAGCA TATGCTCACT ATGAGACCAA ACTCTCGGAA 
AAGGCTTTGT TTGATTTGTT GGTTCCTCTC AGAGAGGGTC AAAGGTGCGT CTCCTTGGCT 
CAGCTTAATC GACTGAAGAA ACTGGCCATT GAGAAATCTG ACCCCTTGAC TCTCACAGAG 
AGAGAGATAA GACGTTTTAT CCGATTGGAC ATTGACACAG ACTCTGCAAC GGATTCATTC 
TCCTTGGAAA ATGAGATGAT GGCTGTCCTG TCACTCAGCA GCAGTGAGGA GGAGATGCAG 
CACAGACTGG CCAACATCAT AGTAGCCACA AACAAATCTG GAGATCCTAT CACTACCGAG 
GATTTAAATG TCAGTGGTCC TTTGGCTATG TTATTGAAAG AAGCCCTGAA ACCTGTCTTG 
ATGCAAACTG TCGAGGGTAC ACCTGTGTTT CTTCACACCA GCCCATTAGT CAATGTTGCC 
CAAGGCGGTC CTTCCATTCT GGCAGACAAA ATGGCTTTAA AAATTGTTGG ATCAGAGGGA 
TTTGTAGTCA CAGAGTCGGG TCATGGAGCT GATGTTGGCT TGGAGAAGTT TTTCAACATT 
AAATGCCTCT ACTCAGGTCT TCAGCCAGAT GTGCTAGTGA TGGTGGCAAC CATTCGTACA 
TTGAAGATGC AAGGTGGAGC ACCTCCTATC CAAGCAGGTT TGCCCCTTCC AAAAGAGTAC 
TTACAAGAGA ACCTCAAGCA GCTGGAAAGA GGCTGTTGTC ATATGAGGAG GCAGGTGGAA 
AAAGCACAAG CATTCGGCTT GCCGGTGATC GTAGCTGTCA ACAAATTCAG CTCTGATACT 
GACGCTGAGC TGGAGCTGGT ATGTGGCCAG GCGAGGCAGG CTGGTGCCTT TGATGCAGTG 
CAGTGCAGCA GCTGGTCTGA AGGGGGCGCT GGAGCTCTGG CTCTGGCCGA GACGGTTCAG 
AGAGCAGCAA AACTACCTGC CTGCTTCAAG TTCCTCTATG ACTTGCAGAC TCCAGCCTCT 
GATAAGTTAA GGACTGTAGC ACAGAAGATG TACGGTCTGA AAGACGTCAA GCTGTCTCCA 
AAAGCAAAGG AAAAACTTGC ACTTTATACA AAACAGGGCT TTGAAAATTT GCCGGTTTGC 
ATAGCCAAGA CACCGCTGTC CTTGAGTAAT GACCCCATGA TGAAGGGGTC GTCCACTGAC 
TTCATGCTGC CCATAACCGA TATCAAAGCC AATGCTGGAG CAGGTTTCCT TTGCTGTCTG 
ACTGGCTCGA CAAATGCAGA AATGGATGAT CCCATGTGGC CCTGTTTCCA TGGCAACAAC 
CTTTACAGTG ACAGCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFD1A ( XM_017352710.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017208199.1 Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1a, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (mthfd1a), transcript variant X2, mRNA.

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CloneID	ODa56730
Clone ID Related Accession (Same CDS sequence)	XM_017352710.2 , XM_021473375.1 , XM_017352710.1
Accession Version	XM_017352710.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	C-1-tetrahydrofolate synthase, cytoplasmic isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGACAGA TGCTGTGCGC TTGCAACAGT TGTTTCTGGA AACAAGACCT CACAGCAGGT 
GAAACAGCGG CTGAAGGAGG AGGTGGAGCA GATGAAGAGT CAGTTTCCAG GTTTCAGGCC 
TGGTCTTGTG GTGCTGCAGG TGGTCATTTG GTTGGAGACA GAGAAGACTC AAACCTCTAC 
ATCAGTATGA AGCTCAAAGC TGCGGCTGAG ATTGGAATCA ATGCGAACCA CATCAGGCTT 
CCTGAAACAG CCACTGAAAA TGAAGTCCTA CGCAACATAG TTGCGGTGAA TGAGAATCCT 
GCATTTCATG GGCTCATTGT GCAGCTGCCG CTCGACTCCA TCAACCCAAT AAACACTGAG 
AAAATCACCA ATGCTGTCGC TCCAGAGAAA GATGTTGATG GCTTGACCAG TATAAATGCG 
GGGAAGCTGT CTCGTGGAGA CCTGGGCGAC TGCTTCATCC CATGCACTCC TAATGGCTGT 
ATGGAGCTTA TCAGCCAGAC TGGTGTGAGT GTGATGGGGA AGAATGCTGT CGTGATCGGC 
CGCAGCAAGA TTGTTGGAGC CCCAATGCAC GATCTGCTCT TGTGGAGCCA CGCAACAGTG 
ACCACCTGCC ACTCGAAGAC CCCTGACCTC GCCGAACAGG TGGGCAAAGC AGATATTTTG 
GTTGTGGGTG CTGGCAGAGC AGAGATGGTG AAGGGCGAGT GGGTGAAGGA GGGGGCCGTG 
GTCATTGACT GCGGCATCAA TCATATACCA GATGAGAGTA AAGCTAGCGG GAAGCGGGTG 
GTGGGTGATG TGCATTACCC CTCAGCTAAA CAGCGAGCTG GATTCATTAC CCCAGTGCCT 
GGTGGAGTCG GACCCATGAC TGTGGCTATG CTGATGAAGA ATACAGTTGA AAGTGCGAAG 
AGGTTTCTGC AAACACACAC TCCTGGCAAG TGGAACTTAG TGTACACAAA GAAGAGACCC 
CATGAGCCTC AGCCCAGCGA TGCTGCAATC TCCTCTTCAT GTGAAAGCAA ACCAGTAGTT 
CAGCTCGCCA GAGAATTGGG ATTGTTTTCT GATGAAGTAG AGTCATACGG CAGGAGCAGG 
GCTAAGATCC GACTGGATGC TTTGAAGCGC ATGGAAAAAC AGCCGAATGG GAAATATGTG 
GTTGTCACCG GAGTTACTTC TTCTCCACTG GGTGAGGGAA AGCGCACCAC TGCTCTCGGA 
CTGGCTCAAG CTTTAGGGGC TCACCTGAAT ACCAACGCTT TCGCTTGTGT TCAAAAGTCC 
TCACTGGAAA CCTGTTTTGG TGCTAGAGGG CCTGCTGTTG GAGGAGGATA CTCTCAAATT 
ATCCCACTGG AGGAGGTCAG CCTTCAGCCT TCAGGCCAGA ATGAGCTCGT CTCTGCTGCC 
GGTAGACTGG TGATGGACAC TGTTAAAGCA TATGCTCACT ATGAGACCAA ACTCTCGGAA 
AAGGCTTTGT TTGATTTGTT GGTTCCTCTC AGAGAGGGTC AAAGGTGCGT CTCCTTGGCT 
CAGCTTAATC GACTGAAGAA ACTGGCCATT GAGAAATCTG ACCCCTTGAC TCTCACAGAG 
AGAGAGATAA GACGTTTTAT CCGATTGGAC ATTGACACAG ACTCTGCAAC GGATTCATTC 
TCCTTGGAAA ATGAGATGAT GGCTGTCCTG TCACTCAGCA GCAGTGAGGA GGAGATGCAG 
CACAGACTGG CCAACATCAT AGTAGCCACA AACAAATCTG GAGATCCTAT CACTACCGAG 
GATTTAAATG TCAGTGGTCC TTTGGCTATG TTATTGAAAG AAGCCCTGAA ACCTGTCTTG 
ATGCAAACTG TCGAGGGTAC ACCTGTGTTT CTTCACACCA GCCCATTAGT CAATGTTGCC 
CAAGGCGGTC CTTCCATTCT GGCAGACAAA ATGGCTTTAA AAATTGTTGG ATCAGAGGGA 
TTTGTAGTCA CAGAGTCGGG TCATGGAGCT GATGTTGGCT TGGAGAAGTT TTTCAACATT 
AAATGCCTCT ACTCAGGTCT TCAGCCAGAT GTGCTAGTGA TGGTGGCAAC CATTCGTACA 
TTGAAGATGC AAGGTGGAGC ACCTCCTATC CAAGCAGGTT TGCCCCTTCC AAAAGAGTAC 
TTACAAGAGA ACCTCAAGCA GCTGGAAAGA GGCTGTTGTC ATATGAGGAG GCAGGTGGAA 
AAAGCACAAG CATTCGGCTT GCCGGTGATC GTAGCTGTCA ACAAATTCAG CTCTGATACT 
GACGCTGAGC TGGAGCTGGT ATGTGGCCAG GCGAGGCAGG CTGGTGCCTT TGATGCAGTG 
CAGTGCAGCA GCTGGTCTGA AGGGGGCGCT GGAGCTCTGG CTCTGGCCGA GACGGTTCAG 
AGAGCAGCAA AACTACCTGC CTGCTTCAAG TTCCTCTATG ACTTGCAGAC TCCAGCCTCT 
GATAAGTTAA GGACTGTAGC ACAGAAGATG TACGGTCTGA AAGACGTCAA GCTGTCTCCA 
AAAGCAAAGG AAAAACTTGC ACTTTATACA AAACAGGGCT TTGAAAATTT GCCGGTTTGC 
ATAGCCAAGA CACCGCTGTC CTTGAGTAAT GACCCCATGA TGAAGGGGTC GTCCACTGAC 
TTCATGCTGC CCATAACCGA TATCAAAGCC AATGCTGGAG CAGGTTTCCT TTGCTGTCTG 
ACTGGCTCGA CAAATGCAGA AATGGATGAT CCCATGTGGC CCTGTTTCCA TGGCAACAAC 
CTTTACAGTG ACAGCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017208199.1
CDS	600..3320
Translation	MGQMLCACNSCFWKQDLTAGETAAEGGGGADEESVSRFQAWSCGAAGGHLVGDREDSNLYISMKLKAAAEIGINANHIRLPETATENEVLRNIVAVNENPAFHGLIVQLPLDSINPINTEKITNAVAPEKDVDGLTSINAGKLSRGDLGDCFIPCTPNGCMELISQTGVSVMGKNAVVIGRSKIVGAPMHDLLLWSHATVTTCHSKTPDLAEQVGKADILVVGAGRAEMVKGEWVKEGAVVIDCGINHIPDESKASGKRVVGDVHYPSAKQRAGFITPVPGGVGPMTVAMLMKNTVESAKRFLQTHTPGKWNLVYTKKRPHEPQPSDAAISSSCESKPVVQLARELGLFSDEVESYGRSRAKIRLDALKRMEKQPNGKYVVVTGVTSSPLGEGKRTTALGLAQALGAHLNTNAFACVQKSSLETCFGARGPAVGGGYSQIIPLEEVSLQPSGQNELVSAAGRLVMDTVKAYAHYETKLSEKALFDLLVPLREGQRCVSLAQLNRLKKLAIEKSDPLTLTEREIRRFIRLDIDTDSATDSFSLENEMMAVLSLSSSEEEMQHRLANIIVATNKSGDPITTEDLNVSGPLAMLLKEALKPVLMQTVEGTPVFLHTSPLVNVAQGGPSILADKMALKIVGSEGFVVTESGHGADVGLEKFFNIKCLYSGLQPDVLVMVATIRTLKMQGGAPPIQAGLPLPKEYLQENLKQLERGCCHMRRQVEKAQAFGLPVIVAVNKFSSDTDAELELVCGQARQAGAFDAVQCSSWSEGGAGALALAETVQRAAKLPACFKFLYDLQTPASDKLRTVAQKMYGLKDVKLSPKAKEKLALYTKQGFENLPVCIAKTPLSLSNDPMMKGSSTDFMLPITDIKANAGAGFLCCLTGSTNAEMDDPMWPCFHGNNLYSDSK

Target ORF information:

RefSeq Version	XM_017352710.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 1a, methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (mthfd1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352710.1

ORF Insert Sequence:

ATGGGACAGA TGCTGTGCGC TTGCAACAGT TGTTTCTGGA AACAAGACCT CACAGCAGGT 
GAAACAGCGG CTGAAGGAGG AGGTGGAGCA GATGAAGAGT CAGTTTCCAG GTTTCAGGCC 
TGGTCTTGTG GTGCTGCAGG TGGTCATTTG GTTGGAGACA GAGAAGACTC AAACCTCTAC 
ATCAGTATGA AGCTCAAAGC TGCGGCTGAG ATTGGAATCA ATGCGAACCA CATCAGGCTT 
CCTGAAACAG CCACTGAAAA TGAAGTCCTA CGCAACATAG TTGCGGTGAA TGAGAATCCT 
GCATTTCATG GGCTCATTGT GCAGCTGCCG CTCGACTCCA TCAACCCAAT AAACACTGAG 
AAAATCACCA ATGCTGTCGC TCCAGAGAAA GATGTTGATG GCTTGACCAG TATAAATGCG 
GGGAAGCTGT CTCGTGGAGA CCTGGGCGAC TGCTTCATCC CATGCACTCC TAATGGCTGT 
ATGGAGCTTA TCAGCCAGAC TGGTGTGAGT GTGATGGGGA AGAATGCTGT CGTGATCGGC 
CGCAGCAAGA TTGTTGGAGC CCCAATGCAC GATCTGCTCT TGTGGAGCCA CGCAACAGTG 
ACCACCTGCC ACTCGAAGAC CCCTGACCTC GCCGAACAGG TGGGCAAAGC AGATATTTTG 
GTTGTGGGTG CTGGCAGAGC AGAGATGGTG AAGGGCGAGT GGGTGAAGGA GGGGGCCGTG 
GTCATTGACT GCGGCATCAA TCATATACCA GATGAGAGTA AAGCTAGCGG GAAGCGGGTG 
GTGGGTGATG TGCATTACCC CTCAGCTAAA CAGCGAGCTG GATTCATTAC CCCAGTGCCT 
GGTGGAGTCG GACCCATGAC TGTGGCTATG CTGATGAAGA ATACAGTTGA AAGTGCGAAG 
AGGTTTCTGC AAACACACAC TCCTGGCAAG TGGAACTTAG TGTACACAAA GAAGAGACCC 
CATGAGCCTC AGCCCAGCGA TGCTGCAATC TCCTCTTCAT GTGAAAGCAA ACCAGTAGTT 
CAGCTCGCCA GAGAATTGGG ATTGTTTTCT GATGAAGTAG AGTCATACGG CAGGAGCAGG 
GCTAAGATCC GACTGGATGC TTTGAAGCGC ATGGAAAAAC AGCCGAATGG GAAATATGTG 
GTTGTCACCG GAGTTACTTC TTCTCCACTG GGTGAGGGAA AGCGCACCAC TGCTCTCGGA 
CTGGCTCAAG CTTTAGGGGC TCACCTGAAT ACCAACGCTT TCGCTTGTGT TCAAAAGTCC 
TCACTGGAAA CCTGTTTTGG TGCTAGAGGG CCTGCTGTTG GAGGAGGATA CTCTCAAATT 
ATCCCACTGG AGGAGGTCAG CCTTCAGCCT TCAGGCCAGA ATGAGCTCGT CTCTGCTGCC 
GGTAGACTGG TGATGGACAC TGTTAAAGCA TATGCTCACT ATGAGACCAA ACTCTCGGAA 
AAGGCTTTGT TTGATTTGTT GGTTCCTCTC AGAGAGGGTC AAAGGTGCGT CTCCTTGGCT 
CAGCTTAATC GACTGAAGAA ACTGGCCATT GAGAAATCTG ACCCCTTGAC TCTCACAGAG 
AGAGAGATAA GACGTTTTAT CCGATTGGAC ATTGACACAG ACTCTGCAAC GGATTCATTC 
TCCTTGGAAA ATGAGATGAT GGCTGTCCTG TCACTCAGCA GCAGTGAGGA GGAGATGCAG 
CACAGACTGG CCAACATCAT AGTAGCCACA AACAAATCTG GAGATCCTAT CACTACCGAG 
GATTTAAATG TCAGTGGTCC TTTGGCTATG TTATTGAAAG AAGCCCTGAA ACCTGTCTTG 
ATGCAAACTG TCGAGGGTAC ACCTGTGTTT CTTCACACCA GCCCATTAGT CAATGTTGCC 
CAAGGCGGTC CTTCCATTCT GGCAGACAAA ATGGCTTTAA AAATTGTTGG ATCAGAGGGA 
TTTGTAGTCA CAGAGTCGGG TCATGGAGCT GATGTTGGCT TGGAGAAGTT TTTCAACATT 
AAATGCCTCT ACTCAGGTCT TCAGCCAGAT GTGCTAGTGA TGGTGGCAAC CATTCGTACA 
TTGAAGATGC AAGGTGGAGC ACCTCCTATC CAAGCAGGTT TGCCCCTTCC AAAAGAGTAC 
TTACAAGAGA ACCTCAAGCA GCTGGAAAGA GGCTGTTGTC ATATGAGGAG GCAGGTGGAA 
AAAGCACAAG CATTCGGCTT GCCGGTGATC GTAGCTGTCA ACAAATTCAG CTCTGATACT 
GACGCTGAGC TGGAGCTGGT ATGTGGCCAG GCGAGGCAGG CTGGTGCCTT TGATGCAGTG 
CAGTGCAGCA GCTGGTCTGA AGGGGGCGCT GGAGCTCTGG CTCTGGCCGA GACGGTTCAG 
AGAGCAGCAA AACTACCTGC CTGCTTCAAG TTCCTCTATG ACTTGCAGAC TCCAGCCTCT 
GATAAGTTAA GGACTGTAGC ACAGAAGATG TACGGTCTGA AAGACGTCAA GCTGTCTCCA 
AAAGCAAAGG AAAAACTTGC ACTTTATACA AAACAGGGCT TTGAAAATTT GCCGGTTTGC 
ATAGCCAAGA CACCGCTGTC CTTGAGTAAT GACCCCATGA TGAAGGGGTC GTCCACTGAC 
TTCATGCTGC CCATAACCGA TATCAAAGCC AATGCTGGAG CAGGTTTCCT TTGCTGTCTG 
ACTGGCTCGA CAAATGCAGA AATGGATGAT CCCATGTGGC CCTGTTTCCA TGGCAACAAC 
CTTTACAGTG ACAGCAAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MTHFD1A cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

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