mfn2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mfn2
Entrez Gene ID	567448
Full Name	mitofusin 2
Synonyms	mg:cb01g09,si:dkeyp-104h9.2,wu:fb79a11
General protein information	Preferred Names mitofusin 2 Names mitofusin-2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005172483.4	XP_005172540.1	mitofusin-2 isoform X1
XM_005172484.3	XP_005172541.1	mitofusin-2 isoform X1
XM_005172484.4	XP_005172541.1	mitofusin-2 isoform X1
XM_005172483.3	XP_005172540.1	mitofusin-2 isoform X1
NM_001128254.2	NP_001121726.1	mitofusin-2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MFN2	XP_003307856.1
Canis lupus familiaris (dog)	MFN2	XP_005618048.1
Bos taurus (cattle)	MFN2	NP_001177198.1
Mus musculus (house mouse)	Mfn2	NP_001272849.1
Rattus norvegicus (Norway rat)	Mfn2	NP_570964.4
Danio rerio (zebrafish)	mfn2	NP_001121726.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001802	XP_321258.5
Caenorhabditis elegans (roundworm)	fzo-1	NP_495161.1
Rattus norvegicus (Norway rat)	LOC100911485	XP_003750134.1
Xenopus tropicalis (tropical clawed frog)	mfn2	NP_001016514.1
	MFN2	XP_002802232.1
Gallus gallus (chicken)	MFN2	XP_004947560.1
Homo sapiens (human)	MFN2	NP_001121132.1
Drosophila melanogaster (fruit fly)	Marf	NP_996357.1

Gene Ontology
Bibliography

Related articles in PubMed

Pla2g6 Deficiency in Zebrafish Leads to Dopaminergic Cell Death, Axonal Degeneration, Increased ?-Synuclein Expression, and Defects in Brain Functions and Pathways.
S?nchez E, Azcona LJ, Pais?n-Ruiz C
Molecular neurobiology(2018 Jan)

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MFN2 mutation status should be investigated in patients presenting with early-onset recessively inherited axonal CMT
Title: Axonal Transport Defects in a Mitofusin 2 Loss of Function Model of Charcot-Marie-Tooth Disease in Zebrafish.

These results highlight the essential role of mitofusin 2 in the motor axon development and demonstrate the potential of zebrafish as a suitable and complementary platform for dissecting pathogenetic mechanisms of MFN2 mutations in vivo.
Title: Developmental defects and neuromuscular alterations due to mitofusin 2 gene (MFN2) silencing in zebrafish: a new model for Charcot-Marie-Tooth type 2A neuropathy.

The following mfn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mfn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa01083	NM_001128254.2 Latest version!	Danio rerio mitofusin 2 (mfn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa01083	XM_005172483.4 Latest version!	Danio rerio mitofusin 2 (mfn2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa01083	XM_005172484.4 Latest version!	Danio rerio mitofusin 2 (mfn2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa01083	XM_005172484.3	Danio rerio mitofusin 2 (mfn2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa01083	XM_005172483.3	Danio rerio mitofusin 2 (mfn2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mfn2 ( NM_001128254.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001121726.1 Danio rerio mitofusin 2 (mfn2), mRNA.

Price & Availability↑

CloneID	ODa01083
Clone ID Related Accession (Same CDS sequence)	XM_005172483.4 , XM_005172484.3 , XM_005172484.4 , XM_005172483.3 , NM_001128254.2
Accession Version	NM_001128254.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-23
Organism	Danio rerio(zebrafish)
Product	mitofusin-2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX548250.8. On Apr 18, 2014 this sequence version replaced NM_001128254.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: GFIL01026300.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTCTCTGG TTTTCCCGCG ACCCAATACC AGTGTGATCG GCAAGAAGGA CAAGCGACTC 
ATGGCTGAGG TGAACGCATC TCCGCTCAAA CACTTCGTCA CTGCCAAGAA GAAGATCAAC 
GGCATCTTTG AACAGCTGGG CGCCTACATC AAAGAAAGCT CAGCGTTTCT AGAAGAAACC 
TACAGCAATG AAGAGCTGGA TCCTGTTACC ACAGAGGAGC AGGTGGCGGA GGTGCGGGGA 
TACCTGTCCA AAGTGGCAGG GATTGGTGAA GTCCTGTCCC GAAGACACAT GAAAGTGGTC 
TTCTTTGGCA GGACCAGTAA CGGAAAGAGC TCCGTCATTA ATGCCATGCT TTGGGATAAA 
GTGTTGCCGT CTGGAATCGG ACACACCACC AACTGCTTCC TGCGAGTGGA AGGCACTGAT 
GGCAATGAGT CTTTCCTGCT CACTGAAGGG TCGGACGAAA GGAAGAGTGT TAAGACGGTG 
AACCAGTTGG CACATGCTTT ACATCAGGAT GAGGATTTGG ATGCTGGCAG CCTGGTGTGT 
GTCATGTGGC CCAAAGCAAA GTGTGCGCTG CTGCGTGATG ACCTGGTACT TGTGGACAGC 
CCTGGTATTG ATGTCACAAC AGAGCTGGAC AGCTGGATCG ACAAATTCTG CCTCGATGCT 
GACGTTTTTG TGCTGGTGGC CAATTCAGAG TCCACTTTAA TGCAAACAGA AAAATCTTTT 
TTTCACAAAG TAAACGAACG CCTCTCCAGC CCTAATATCT TCATCCTGAA CAACCGCTGG 
GACGCATCGG CCAATGAGCC AGAGTACATG GAAGAGGTGA GGCGGCAGCA CATGGACCGC 
TGCACTAGTT TTTTAGTGGA TGAGCTGCGG GTGGTGGATC GCTCTCATGC CGGGGATCGA 
ATCTTCTTTG TCTCTGCTAA AGAAGTTCTT CAGGCGCGTG TACAGAAGGC CCAGGGAATG 
CCTGAAGCAG GTGGTGCATT AGCTGAAGGT TTTCAAGCCA GAATGTTTGA GTTTCAGAAC 
TTTGAAAGGC GTTTTGAGGA GTGTATCTCG CAGTCAGCAG TGAAGACGAA GTTTGAGCAG 
CACACGACGA GAGCTAAACA GATTTCTGAA GCTCTGCGTC TGATAATGGA TTCTGTGCAC 
ATCGCAGCTC AAGAGCAGAG GATTCATTGC TTGGAGATGA AGGAGGAGAG GGAGGACAGG 
CTGGAGTTCA TTGATAAACA GCTGGATCTG CTCACTCTCG ACTGCAAGAG CAAAATCAAG 
AAGATCACAG AGGAGGTGGA GAAACAGGTT TCCAATGCTA TGGCAGAGGA GATCCGCAGG 
CTGGCGGTAC TGGTGGATGA CTTTCACATG GACTTCCATC CTTCACCAGT CGTGCTCAAA 
GTCTACAAGA ACGAGCTGCA CAGGCACATA GAGGAAGGTC TTGGCAGGAA CATGTCGGAG 
AGATGCTCCA GTGCAATAAC CGTAGCTCTG CAAACCACAC AGACTGACAT GATCGACGGT 
CTGAAGCCAC TGCTGCCTAT CACGATTCGG GAGCAGGTGG ATAAGATGGT GCCCAGACAG 
TGTTTCGCCC TCAGCTACGA CCTCAACTGT GACAAACTCT GCAGCGACTT CCAGGAGGAC 
ATCGGCTTTC ACTTCTCCCT CGGCTGGACG ATGCTGGTCA ACCGCTTCCT GGGCCCCAAA 
AACACACGTC GCGCTCTAAT GGGCTACAAC GACCAGGTTC CCAGATCCCT GGCCATTACT 
CCGGTCAGCA CAAGTATGCC TCCATTCCCT CAAGGATCTA TGACCCAGGA AGAGCTTATG 
GTTTCTATGG TGACAGGACT GGCATCACTC ACCTCTCGGA CATCAATGGG CATCATAGTT 
GTTGGAGGAG TGATCTGGAA AGCAGTGGGC TGGAGGCTCA TTGCTCTGTC TGTGGGTCTC 
TACGGGCTGC TGTACGTGTA TGAGAGACTC ACCTGGACCA CCAGAGCCAA AGAGAGAACA 
TTTAAGAGGC AGTTTGTGGA TTACGCCAGC GAGAAACTGC AGCTCATCGT CAGCTACACC 
GGCTCCAACT GCAGCCATCA AGTTCAGCAG GAGCTGGCCA GCACCTTCTC TCAGCTGTGC 
CAGCAGGTGG ACGTCACCAG ACAGCAGCTG GAGGATGAAA TTGGGGATTT GAACAGCAAG 
ATTGAACAGC TGGACACCCT GCAGAGTAAA GCCAAACTGC TCAGGAATAA GGCGGGCTGG 
CTGGACAGTG AGCTCAACAT GTTCATTCAG CAGTATCTTC AACAGGGGCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121726.1
CDS	43..2316
Translation	MSLVFPRPNTSVIGKKDKRLMAEVNASPLKHFVTAKKKINGIFEQLGAYIKESSAFLEETYSNEELDPVTTEEQVAEVRGYLSKVAGIGEVLSRRHMKVVFFGRTSNGKSSVINAMLWDKVLPSGIGHTTNCFLRVEGTDGNESFLLTEGSDERKSVKTVNQLAHALHQDEDLDAGSLVCVMWPKAKCALLRDDLVLVDSPGIDVTTELDSWIDKFCLDADVFVLVANSESTLMQTEKSFFHKVNERLSSPNIFILNNRWDASANEPEYMEEVRRQHMDRCTSFLVDELRVVDRSHAGDRIFFVSAKEVLQARVQKAQGMPEAGGALAEGFQARMFEFQNFERRFEECISQSAVKTKFEQHTTRAKQISEALRLIMDSVHIAAQEQRIHCLEMKEEREDRLEFIDKQLDLLTLDCKSKIKKITEEVEKQVSNAMAEEIRRLAVLVDDFHMDFHPSPVVLKVYKNELHRHIEEGLGRNMSERCSSAITVALQTTQTDMIDGLKPLLPITIREQVDKMVPRQCFALSYDLNCDKLCSDFQEDIGFHFSLGWTMLVNRFLGPKNTRRALMGYNDQVPRSLAITPVSTSMPPFPQGSMTQEELMVSMVTGLASLTSRTSMGIIVVGGVIWKAVGWRLIALSVGLYGLLYVYERLTWTTRAKERTFKRQFVDYASEKLQLIVSYTGSNCSHQVQQELASTFSQLCQQVDVTRQQLEDEIGDLNSKIEQLDTLQSKAKLLRNKAGWLDSELNMFIQQYLQQGR

Target ORF information:

RefSeq Version	NM_001128254.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitofusin 2 (mfn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128254.2

ORF Insert Sequence:

ATGTCTCTGG TTTTCCCGCG ACCCAATACC AGTGTGATCG GCAAGAAGGA CAAGCGACTC 
ATGGCTGAGG TGAACGCATC TCCGCTCAAA CACTTCGTCA CTGCCAAGAA GAAGATCAAC 
GGCATCTTTG AACAGCTGGG CGCCTACATC AAAGAAAGCT CAGCGTTTCT AGAAGAAACC 
TACAGCAATG AAGAGCTGGA TCCTGTTACC ACAGAGGAGC AGGTGGCGGA GGTGCGGGGA 
TACCTGTCCA AAGTGGCAGG GATTGGTGAA GTCCTGTCCC GAAGACACAT GAAAGTGGTC 
TTCTTTGGCA GGACCAGTAA CGGAAAGAGC TCCGTCATTA ATGCCATGCT TTGGGATAAA 
GTGTTGCCGT CTGGAATCGG ACACACCACC AACTGCTTCC TGCGAGTGGA AGGCACTGAT 
GGCAATGAGT CTTTCCTGCT CACTGAAGGG TCGGACGAAA GGAAGAGTGT TAAGACGGTG 
AACCAGTTGG CACATGCTTT ACATCAGGAT GAGGATTTGG ATGCTGGCAG CCTGGTGTGT 
GTCATGTGGC CCAAAGCAAA GTGTGCGCTG CTGCGTGATG ACCTGGTACT TGTGGACAGC 
CCTGGTATTG ATGTCACAAC AGAGCTGGAC AGCTGGATCG ACAAATTCTG CCTCGATGCT 
GACGTTTTTG TGCTGGTGGC CAATTCAGAG TCCACTTTAA TGCAAACAGA AAAATCTTTT 
TTTCACAAAG TAAACGAACG CCTCTCCAGC CCTAATATCT TCATCCTGAA CAACCGCTGG 
GACGCATCGG CCAATGAGCC AGAGTACATG GAAGAGGTGA GGCGGCAGCA CATGGACCGC 
TGCACTAGTT TTTTAGTGGA TGAGCTGCGG GTGGTGGATC GCTCTCATGC CGGGGATCGA 
ATCTTCTTTG TCTCTGCTAA AGAAGTTCTT CAGGCGCGTG TACAGAAGGC CCAGGGAATG 
CCTGAAGCAG GTGGTGCATT AGCTGAAGGT TTTCAAGCCA GAATGTTTGA GTTTCAGAAC 
TTTGAAAGGC GTTTTGAGGA GTGTATCTCG CAGTCAGCAG TGAAGACGAA GTTTGAGCAG 
CACACGACGA GAGCTAAACA GATTTCTGAA GCTCTGCGTC TGATAATGGA TTCTGTGCAC 
ATCGCAGCTC AAGAGCAGAG GATTCATTGC TTGGAGATGA AGGAGGAGAG GGAGGACAGG 
CTGGAGTTCA TTGATAAACA GCTGGATCTG CTCACTCTCG ACTGCAAGAG CAAAATCAAG 
AAGATCACAG AGGAGGTGGA GAAACAGGTT TCCAATGCTA TGGCAGAGGA GATCCGCAGG 
CTGGCGGTAC TGGTGGATGA CTTTCACATG GACTTCCATC CTTCACCAGT CGTGCTCAAA 
GTCTACAAGA ACGAGCTGCA CAGGCACATA GAGGAAGGTC TTGGCAGGAA CATGTCGGAG 
AGATGCTCCA GTGCAATAAC CGTAGCTCTG CAAACCACAC AGACTGACAT GATCGACGGT 
CTGAAGCCAC TGCTGCCTAT CACGATTCGG GAGCAGGTGG ATAAGATGGT GCCCAGACAG 
TGTTTCGCCC TCAGCTACGA CCTCAACTGT GACAAACTCT GCAGCGACTT CCAGGAGGAC 
ATCGGCTTTC ACTTCTCCCT CGGCTGGACG ATGCTGGTCA ACCGCTTCCT GGGCCCCAAA 
AACACACGTC GCGCTCTAAT GGGCTACAAC GACCAGGTTC CCAGATCCCT GGCCATTACT 
CCGGTCAGCA CAAGTATGCC TCCATTCCCT CAAGGATCTA TGACCCAGGA AGAGCTTATG 
GTTTCTATGG TGACAGGACT GGCATCACTC ACCTCTCGGA CATCAATGGG CATCATAGTT 
GTTGGAGGAG TGATCTGGAA AGCAGTGGGC TGGAGGCTCA TTGCTCTGTC TGTGGGTCTC 
TACGGGCTGC TGTACGTGTA TGAGAGACTC ACCTGGACCA CCAGAGCCAA AGAGAGAACA 
TTTAAGAGGC AGTTTGTGGA TTACGCCAGC GAGAAACTGC AGCTCATCGT CAGCTACACC 
GGCTCCAACT GCAGCCATCA AGTTCAGCAG GAGCTGGCCA GCACCTTCTC TCAGCTGTGC 
CAGCAGGTGG ACGTCACCAG ACAGCAGCTG GAGGATGAAA TTGGGGATTT GAACAGCAAG 
ATTGAACAGC TGGACACCCT GCAGAGTAAA GCCAAACTGC TCAGGAATAA GGCGGGCTGG 
CTGGACAGTG AGCTCAACAT GTTCATTCAG CAGTATCTTC AACAGGGGCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mfn2 ( XM_005172483.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172540.1 Danio rerio mitofusin 2 (mfn2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa01083
Clone ID Related Accession (Same CDS sequence)	XM_005172483.4 , XM_005172484.3 , XM_005172484.4 , XM_005172483.3 , NM_001128254.2
Accession Version	XM_005172483.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mitofusin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172483.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGG TTTTCCCGCG ACCCAATACC AGTGTGATCG GCAAGAAGGA CAAGCGACTC 
ATGGCTGAGG TGAACGCATC TCCGCTCAAA CACTTCGTCA CTGCCAAGAA GAAGATCAAC 
GGCATCTTTG AACAGCTGGG CGCCTACATC AAAGAAAGCT CAGCGTTTCT AGAAGAAACC 
TACAGCAATG AAGAGCTGGA TCCTGTTACC ACAGAGGAGC AGGTGGCGGA GGTGCGGGGA 
TACCTGTCCA AAGTGGCAGG GATTGGTGAA GTCCTGTCCC GAAGACACAT GAAAGTGGTC 
TTCTTTGGCA GGACCAGTAA CGGAAAGAGC TCCGTCATTA ATGCCATGCT TTGGGATAAA 
GTGTTGCCGT CTGGAATCGG ACACACCACC AACTGCTTCC TGCGAGTGGA AGGCACTGAT 
GGCAATGAGT CTTTCCTGCT CACTGAAGGG TCGGACGAAA GGAAGAGTGT TAAGACGGTG 
AACCAGTTGG CACATGCTTT ACATCAGGAT GAGGATTTGG ATGCTGGCAG CCTGGTGTGT 
GTCATGTGGC CCAAAGCAAA GTGTGCGCTG CTGCGTGATG ACCTGGTACT TGTGGACAGC 
CCTGGTATTG ATGTCACAAC AGAGCTGGAC AGCTGGATCG ACAAATTCTG CCTCGATGCT 
GACGTTTTTG TGCTGGTGGC CAATTCAGAG TCCACTTTAA TGCAAACAGA AAAATCTTTT 
TTTCACAAAG TAAACGAACG CCTCTCCAGC CCTAATATCT TCATCCTGAA CAACCGCTGG 
GACGCATCGG CCAATGAGCC AGAGTACATG GAAGAGGTGA GGCGGCAGCA CATGGACCGC 
TGCACTAGTT TTTTAGTGGA TGAGCTGCGG GTGGTGGATC GCTCTCATGC CGGGGATCGA 
ATCTTCTTTG TCTCTGCTAA AGAAGTTCTT CAGGCGCGTG TACAGAAGGC CCAGGGAATG 
CCTGAAGCAG GTGGTGCATT AGCTGAAGGT TTTCAAGCCA GAATGTTTGA GTTTCAGAAC 
TTTGAAAGGC GTTTTGAGGA GTGTATCTCG CAGTCAGCAG TGAAGACGAA GTTTGAGCAG 
CACACGACGA GAGCTAAACA GATTTCTGAA GCTCTGCGTC TGATAATGGA TTCTGTGCAC 
ATCGCAGCTC AAGAGCAGAG GATTCATTGC TTGGAGATGA AGGAGGAGAG GGAGGACAGG 
CTGGAGTTCA TTGATAAACA GCTGGATCTG CTCACTCTCG ACTGCAAGAG CAAAATCAAG 
AAGATCACAG AGGAGGTGGA GAAACAGGTT TCCAATGCTA TGGCAGAGGA GATCCGCAGG 
CTGGCGGTAC TGGTGGATGA CTTTCACATG GACTTCCATC CTTCACCAGT CGTGCTCAAA 
GTCTACAAGA ACGAGCTGCA CAGGCACATA GAGGAAGGTC TTGGCAGGAA CATGTCGGAG 
AGATGCTCCA GTGCAATAAC CGTAGCTCTG CAAACCACAC AGACTGACAT GATCGACGGT 
CTGAAGCCAC TGCTGCCTAT CACGATTCGG GAGCAGGTGG ATAAGATGGT GCCCAGACAG 
TGTTTCGCCC TCAGCTACGA CCTCAACTGT GACAAACTCT GCAGCGACTT CCAGGAGGAC 
ATCGGCTTTC ACTTCTCCCT CGGCTGGACG ATGCTGGTCA ACCGCTTCCT GGGCCCCAAA 
AACACACGTC GCGCTCTAAT GGGCTACAAC GACCAGGTTC CCAGATCCCT GGCCATTACT 
CCGGTCAGCA CAAGTATGCC TCCATTCCCT CAAGGATCTA TGACCCAGGA AGAGCTTATG 
GTTTCTATGG TGACAGGACT GGCATCACTC ACCTCTCGGA CATCAATGGG CATCATAGTT 
GTTGGAGGAG TGATCTGGAA AGCAGTGGGC TGGAGGCTCA TTGCTCTGTC TGTGGGTCTC 
TACGGGCTGC TGTACGTGTA TGAGAGACTC ACCTGGACCA CCAGAGCCAA AGAGAGAACA 
TTTAAGAGGC AGTTTGTGGA TTACGCCAGC GAGAAACTGC AGCTCATCGT CAGCTACACC 
GGCTCCAACT GCAGCCATCA AGTTCAGCAG GAGCTGGCCA GCACCTTCTC TCAGCTGTGC 
CAGCAGGTGG ACGTCACCAG ACAGCAGCTG GAGGATGAAA TTGGGGATTT GAACAGCAAG 
ATTGAACAGC TGGACACCCT GCAGAGTAAA GCCAAACTGC TCAGGAATAA GGCGGGCTGG 
CTGGACAGTG AGCTCAACAT GTTCATTCAG CAGTATCTTC AACAGGGGCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172540.1
CDS	422..2695
Translation	MSLVFPRPNTSVIGKKDKRLMAEVNASPLKHFVTAKKKINGIFEQLGAYIKESSAFLEETYSNEELDPVTTEEQVAEVRGYLSKVAGIGEVLSRRHMKVVFFGRTSNGKSSVINAMLWDKVLPSGIGHTTNCFLRVEGTDGNESFLLTEGSDERKSVKTVNQLAHALHQDEDLDAGSLVCVMWPKAKCALLRDDLVLVDSPGIDVTTELDSWIDKFCLDADVFVLVANSESTLMQTEKSFFHKVNERLSSPNIFILNNRWDASANEPEYMEEVRRQHMDRCTSFLVDELRVVDRSHAGDRIFFVSAKEVLQARVQKAQGMPEAGGALAEGFQARMFEFQNFERRFEECISQSAVKTKFEQHTTRAKQISEALRLIMDSVHIAAQEQRIHCLEMKEEREDRLEFIDKQLDLLTLDCKSKIKKITEEVEKQVSNAMAEEIRRLAVLVDDFHMDFHPSPVVLKVYKNELHRHIEEGLGRNMSERCSSAITVALQTTQTDMIDGLKPLLPITIREQVDKMVPRQCFALSYDLNCDKLCSDFQEDIGFHFSLGWTMLVNRFLGPKNTRRALMGYNDQVPRSLAITPVSTSMPPFPQGSMTQEELMVSMVTGLASLTSRTSMGIIVVGGVIWKAVGWRLIALSVGLYGLLYVYERLTWTTRAKERTFKRQFVDYASEKLQLIVSYTGSNCSHQVQQELASTFSQLCQQVDVTRQQLEDEIGDLNSKIEQLDTLQSKAKLLRNKAGWLDSELNMFIQQYLQQGR

Target ORF information:

RefSeq Version	XM_005172483.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitofusin 2 (mfn2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172483.4

ORF Insert Sequence:

ATGTCTCTGG TTTTCCCGCG ACCCAATACC AGTGTGATCG GCAAGAAGGA CAAGCGACTC 
ATGGCTGAGG TGAACGCATC TCCGCTCAAA CACTTCGTCA CTGCCAAGAA GAAGATCAAC 
GGCATCTTTG AACAGCTGGG CGCCTACATC AAAGAAAGCT CAGCGTTTCT AGAAGAAACC 
TACAGCAATG AAGAGCTGGA TCCTGTTACC ACAGAGGAGC AGGTGGCGGA GGTGCGGGGA 
TACCTGTCCA AAGTGGCAGG GATTGGTGAA GTCCTGTCCC GAAGACACAT GAAAGTGGTC 
TTCTTTGGCA GGACCAGTAA CGGAAAGAGC TCCGTCATTA ATGCCATGCT TTGGGATAAA 
GTGTTGCCGT CTGGAATCGG ACACACCACC AACTGCTTCC TGCGAGTGGA AGGCACTGAT 
GGCAATGAGT CTTTCCTGCT CACTGAAGGG TCGGACGAAA GGAAGAGTGT TAAGACGGTG 
AACCAGTTGG CACATGCTTT ACATCAGGAT GAGGATTTGG ATGCTGGCAG CCTGGTGTGT 
GTCATGTGGC CCAAAGCAAA GTGTGCGCTG CTGCGTGATG ACCTGGTACT TGTGGACAGC 
CCTGGTATTG ATGTCACAAC AGAGCTGGAC AGCTGGATCG ACAAATTCTG CCTCGATGCT 
GACGTTTTTG TGCTGGTGGC CAATTCAGAG TCCACTTTAA TGCAAACAGA AAAATCTTTT 
TTTCACAAAG TAAACGAACG CCTCTCCAGC CCTAATATCT TCATCCTGAA CAACCGCTGG 
GACGCATCGG CCAATGAGCC AGAGTACATG GAAGAGGTGA GGCGGCAGCA CATGGACCGC 
TGCACTAGTT TTTTAGTGGA TGAGCTGCGG GTGGTGGATC GCTCTCATGC CGGGGATCGA 
ATCTTCTTTG TCTCTGCTAA AGAAGTTCTT CAGGCGCGTG TACAGAAGGC CCAGGGAATG 
CCTGAAGCAG GTGGTGCATT AGCTGAAGGT TTTCAAGCCA GAATGTTTGA GTTTCAGAAC 
TTTGAAAGGC GTTTTGAGGA GTGTATCTCG CAGTCAGCAG TGAAGACGAA GTTTGAGCAG 
CACACGACGA GAGCTAAACA GATTTCTGAA GCTCTGCGTC TGATAATGGA TTCTGTGCAC 
ATCGCAGCTC AAGAGCAGAG GATTCATTGC TTGGAGATGA AGGAGGAGAG GGAGGACAGG 
CTGGAGTTCA TTGATAAACA GCTGGATCTG CTCACTCTCG ACTGCAAGAG CAAAATCAAG 
AAGATCACAG AGGAGGTGGA GAAACAGGTT TCCAATGCTA TGGCAGAGGA GATCCGCAGG 
CTGGCGGTAC TGGTGGATGA CTTTCACATG GACTTCCATC CTTCACCAGT CGTGCTCAAA 
GTCTACAAGA ACGAGCTGCA CAGGCACATA GAGGAAGGTC TTGGCAGGAA CATGTCGGAG 
AGATGCTCCA GTGCAATAAC CGTAGCTCTG CAAACCACAC AGACTGACAT GATCGACGGT 
CTGAAGCCAC TGCTGCCTAT CACGATTCGG GAGCAGGTGG ATAAGATGGT GCCCAGACAG 
TGTTTCGCCC TCAGCTACGA CCTCAACTGT GACAAACTCT GCAGCGACTT CCAGGAGGAC 
ATCGGCTTTC ACTTCTCCCT CGGCTGGACG ATGCTGGTCA ACCGCTTCCT GGGCCCCAAA 
AACACACGTC GCGCTCTAAT GGGCTACAAC GACCAGGTTC CCAGATCCCT GGCCATTACT 
CCGGTCAGCA CAAGTATGCC TCCATTCCCT CAAGGATCTA TGACCCAGGA AGAGCTTATG 
GTTTCTATGG TGACAGGACT GGCATCACTC ACCTCTCGGA CATCAATGGG CATCATAGTT 
GTTGGAGGAG TGATCTGGAA AGCAGTGGGC TGGAGGCTCA TTGCTCTGTC TGTGGGTCTC 
TACGGGCTGC TGTACGTGTA TGAGAGACTC ACCTGGACCA CCAGAGCCAA AGAGAGAACA 
TTTAAGAGGC AGTTTGTGGA TTACGCCAGC GAGAAACTGC AGCTCATCGT CAGCTACACC 
GGCTCCAACT GCAGCCATCA AGTTCAGCAG GAGCTGGCCA GCACCTTCTC TCAGCTGTGC 
CAGCAGGTGG ACGTCACCAG ACAGCAGCTG GAGGATGAAA TTGGGGATTT GAACAGCAAG 
ATTGAACAGC TGGACACCCT GCAGAGTAAA GCCAAACTGC TCAGGAATAA GGCGGGCTGG 
CTGGACAGTG AGCTCAACAT GTTCATTCAG CAGTATCTTC AACAGGGGCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mfn2 ( XM_005172484.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172541.1 Danio rerio mitofusin 2 (mfn2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa01083
Clone ID Related Accession (Same CDS sequence)	XM_005172483.4 , XM_005172484.3 , XM_005172484.4 , XM_005172483.3 , NM_001128254.2
Accession Version	XM_005172484.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	mitofusin-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172484.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGG TTTTCCCGCG ACCCAATACC AGTGTGATCG GCAAGAAGGA CAAGCGACTC 
ATGGCTGAGG TGAACGCATC TCCGCTCAAA CACTTCGTCA CTGCCAAGAA GAAGATCAAC 
GGCATCTTTG AACAGCTGGG CGCCTACATC AAAGAAAGCT CAGCGTTTCT AGAAGAAACC 
TACAGCAATG AAGAGCTGGA TCCTGTTACC ACAGAGGAGC AGGTGGCGGA GGTGCGGGGA 
TACCTGTCCA AAGTGGCAGG GATTGGTGAA GTCCTGTCCC GAAGACACAT GAAAGTGGTC 
TTCTTTGGCA GGACCAGTAA CGGAAAGAGC TCCGTCATTA ATGCCATGCT TTGGGATAAA 
GTGTTGCCGT CTGGAATCGG ACACACCACC AACTGCTTCC TGCGAGTGGA AGGCACTGAT 
GGCAATGAGT CTTTCCTGCT CACTGAAGGG TCGGACGAAA GGAAGAGTGT TAAGACGGTG 
AACCAGTTGG CACATGCTTT ACATCAGGAT GAGGATTTGG ATGCTGGCAG CCTGGTGTGT 
GTCATGTGGC CCAAAGCAAA GTGTGCGCTG CTGCGTGATG ACCTGGTACT TGTGGACAGC 
CCTGGTATTG ATGTCACAAC AGAGCTGGAC AGCTGGATCG ACAAATTCTG CCTCGATGCT 
GACGTTTTTG TGCTGGTGGC CAATTCAGAG TCCACTTTAA TGCAAACAGA AAAATCTTTT 
TTTCACAAAG TAAACGAACG CCTCTCCAGC CCTAATATCT TCATCCTGAA CAACCGCTGG 
GACGCATCGG CCAATGAGCC AGAGTACATG GAAGAGGTGA GGCGGCAGCA CATGGACCGC 
TGCACTAGTT TTTTAGTGGA TGAGCTGCGG GTGGTGGATC GCTCTCATGC CGGGGATCGA 
ATCTTCTTTG TCTCTGCTAA AGAAGTTCTT CAGGCGCGTG TACAGAAGGC CCAGGGAATG 
CCTGAAGCAG GTGGTGCATT AGCTGAAGGT TTTCAAGCCA GAATGTTTGA GTTTCAGAAC 
TTTGAAAGGC GTTTTGAGGA GTGTATCTCG CAGTCAGCAG TGAAGACGAA GTTTGAGCAG 
CACACGACGA GAGCTAAACA GATTTCTGAA GCTCTGCGTC TGATAATGGA TTCTGTGCAC 
ATCGCAGCTC AAGAGCAGAG GATTCATTGC TTGGAGATGA AGGAGGAGAG GGAGGACAGG 
CTGGAGTTCA TTGATAAACA GCTGGATCTG CTCACTCTCG ACTGCAAGAG CAAAATCAAG 
AAGATCACAG AGGAGGTGGA GAAACAGGTT TCCAATGCTA TGGCAGAGGA GATCCGCAGG 
CTGGCGGTAC TGGTGGATGA CTTTCACATG GACTTCCATC CTTCACCAGT CGTGCTCAAA 
GTCTACAAGA ACGAGCTGCA CAGGCACATA GAGGAAGGTC TTGGCAGGAA CATGTCGGAG 
AGATGCTCCA GTGCAATAAC CGTAGCTCTG CAAACCACAC AGACTGACAT GATCGACGGT 
CTGAAGCCAC TGCTGCCTAT CACGATTCGG GAGCAGGTGG ATAAGATGGT GCCCAGACAG 
TGTTTCGCCC TCAGCTACGA CCTCAACTGT GACAAACTCT GCAGCGACTT CCAGGAGGAC 
ATCGGCTTTC ACTTCTCCCT CGGCTGGACG ATGCTGGTCA ACCGCTTCCT GGGCCCCAAA 
AACACACGTC GCGCTCTAAT GGGCTACAAC GACCAGGTTC CCAGATCCCT GGCCATTACT 
CCGGTCAGCA CAAGTATGCC TCCATTCCCT CAAGGATCTA TGACCCAGGA AGAGCTTATG 
GTTTCTATGG TGACAGGACT GGCATCACTC ACCTCTCGGA CATCAATGGG CATCATAGTT 
GTTGGAGGAG TGATCTGGAA AGCAGTGGGC TGGAGGCTCA TTGCTCTGTC TGTGGGTCTC 
TACGGGCTGC TGTACGTGTA TGAGAGACTC ACCTGGACCA CCAGAGCCAA AGAGAGAACA 
TTTAAGAGGC AGTTTGTGGA TTACGCCAGC GAGAAACTGC AGCTCATCGT CAGCTACACC 
GGCTCCAACT GCAGCCATCA AGTTCAGCAG GAGCTGGCCA GCACCTTCTC TCAGCTGTGC 
CAGCAGGTGG ACGTCACCAG ACAGCAGCTG GAGGATGAAA TTGGGGATTT GAACAGCAAG 
ATTGAACAGC TGGACACCCT GCAGAGTAAA GCCAAACTGC TCAGGAATAA GGCGGGCTGG 
CTGGACAGTG AGCTCAACAT GTTCATTCAG CAGTATCTTC AACAGGGGCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172541.1
CDS	279..2552
Translation	MSLVFPRPNTSVIGKKDKRLMAEVNASPLKHFVTAKKKINGIFEQLGAYIKESSAFLEETYSNEELDPVTTEEQVAEVRGYLSKVAGIGEVLSRRHMKVVFFGRTSNGKSSVINAMLWDKVLPSGIGHTTNCFLRVEGTDGNESFLLTEGSDERKSVKTVNQLAHALHQDEDLDAGSLVCVMWPKAKCALLRDDLVLVDSPGIDVTTELDSWIDKFCLDADVFVLVANSESTLMQTEKSFFHKVNERLSSPNIFILNNRWDASANEPEYMEEVRRQHMDRCTSFLVDELRVVDRSHAGDRIFFVSAKEVLQARVQKAQGMPEAGGALAEGFQARMFEFQNFERRFEECISQSAVKTKFEQHTTRAKQISEALRLIMDSVHIAAQEQRIHCLEMKEEREDRLEFIDKQLDLLTLDCKSKIKKITEEVEKQVSNAMAEEIRRLAVLVDDFHMDFHPSPVVLKVYKNELHRHIEEGLGRNMSERCSSAITVALQTTQTDMIDGLKPLLPITIREQVDKMVPRQCFALSYDLNCDKLCSDFQEDIGFHFSLGWTMLVNRFLGPKNTRRALMGYNDQVPRSLAITPVSTSMPPFPQGSMTQEELMVSMVTGLASLTSRTSMGIIVVGGVIWKAVGWRLIALSVGLYGLLYVYERLTWTTRAKERTFKRQFVDYASEKLQLIVSYTGSNCSHQVQQELASTFSQLCQQVDVTRQQLEDEIGDLNSKIEQLDTLQSKAKLLRNKAGWLDSELNMFIQQYLQQGR

Target ORF information:

RefSeq Version	XM_005172484.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mitofusin 2 (mfn2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172484.3

ORF Insert Sequence:

ATGTCTCTGG TTTTCCCGCG ACCCAATACC AGTGTGATCG GCAAGAAGGA CAAGCGACTC 
ATGGCTGAGG TGAACGCATC TCCGCTCAAA CACTTCGTCA CTGCCAAGAA GAAGATCAAC 
GGCATCTTTG AACAGCTGGG CGCCTACATC AAAGAAAGCT CAGCGTTTCT AGAAGAAACC 
TACAGCAATG AAGAGCTGGA TCCTGTTACC ACAGAGGAGC AGGTGGCGGA GGTGCGGGGA 
TACCTGTCCA AAGTGGCAGG GATTGGTGAA GTCCTGTCCC GAAGACACAT GAAAGTGGTC 
TTCTTTGGCA GGACCAGTAA CGGAAAGAGC TCCGTCATTA ATGCCATGCT TTGGGATAAA 
GTGTTGCCGT CTGGAATCGG ACACACCACC AACTGCTTCC TGCGAGTGGA AGGCACTGAT 
GGCAATGAGT CTTTCCTGCT CACTGAAGGG TCGGACGAAA GGAAGAGTGT TAAGACGGTG 
AACCAGTTGG CACATGCTTT ACATCAGGAT GAGGATTTGG ATGCTGGCAG CCTGGTGTGT 
GTCATGTGGC CCAAAGCAAA GTGTGCGCTG CTGCGTGATG ACCTGGTACT TGTGGACAGC 
CCTGGTATTG ATGTCACAAC AGAGCTGGAC AGCTGGATCG ACAAATTCTG CCTCGATGCT 
GACGTTTTTG TGCTGGTGGC CAATTCAGAG TCCACTTTAA TGCAAACAGA AAAATCTTTT 
TTTCACAAAG TAAACGAACG CCTCTCCAGC CCTAATATCT TCATCCTGAA CAACCGCTGG 
GACGCATCGG CCAATGAGCC AGAGTACATG GAAGAGGTGA GGCGGCAGCA CATGGACCGC 
TGCACTAGTT TTTTAGTGGA TGAGCTGCGG GTGGTGGATC GCTCTCATGC CGGGGATCGA 
ATCTTCTTTG TCTCTGCTAA AGAAGTTCTT CAGGCGCGTG TACAGAAGGC CCAGGGAATG 
CCTGAAGCAG GTGGTGCATT AGCTGAAGGT TTTCAAGCCA GAATGTTTGA GTTTCAGAAC 
TTTGAAAGGC GTTTTGAGGA GTGTATCTCG CAGTCAGCAG TGAAGACGAA GTTTGAGCAG 
CACACGACGA GAGCTAAACA GATTTCTGAA GCTCTGCGTC TGATAATGGA TTCTGTGCAC 
ATCGCAGCTC AAGAGCAGAG GATTCATTGC TTGGAGATGA AGGAGGAGAG GGAGGACAGG 
CTGGAGTTCA TTGATAAACA GCTGGATCTG CTCACTCTCG ACTGCAAGAG CAAAATCAAG 
AAGATCACAG AGGAGGTGGA GAAACAGGTT TCCAATGCTA TGGCAGAGGA GATCCGCAGG 
CTGGCGGTAC TGGTGGATGA CTTTCACATG GACTTCCATC CTTCACCAGT CGTGCTCAAA 
GTCTACAAGA ACGAGCTGCA CAGGCACATA GAGGAAGGTC TTGGCAGGAA CATGTCGGAG 
AGATGCTCCA GTGCAATAAC CGTAGCTCTG CAAACCACAC AGACTGACAT GATCGACGGT 
CTGAAGCCAC TGCTGCCTAT CACGATTCGG GAGCAGGTGG ATAAGATGGT GCCCAGACAG 
TGTTTCGCCC TCAGCTACGA CCTCAACTGT GACAAACTCT GCAGCGACTT CCAGGAGGAC 
ATCGGCTTTC ACTTCTCCCT CGGCTGGACG ATGCTGGTCA ACCGCTTCCT GGGCCCCAAA 
AACACACGTC GCGCTCTAAT GGGCTACAAC GACCAGGTTC CCAGATCCCT GGCCATTACT 
CCGGTCAGCA CAAGTATGCC TCCATTCCCT CAAGGATCTA TGACCCAGGA AGAGCTTATG 
GTTTCTATGG TGACAGGACT GGCATCACTC ACCTCTCGGA CATCAATGGG CATCATAGTT 
GTTGGAGGAG TGATCTGGAA AGCAGTGGGC TGGAGGCTCA TTGCTCTGTC TGTGGGTCTC 
TACGGGCTGC TGTACGTGTA TGAGAGACTC ACCTGGACCA CCAGAGCCAA AGAGAGAACA 
TTTAAGAGGC AGTTTGTGGA TTACGCCAGC GAGAAACTGC AGCTCATCGT CAGCTACACC 
GGCTCCAACT GCAGCCATCA AGTTCAGCAG GAGCTGGCCA GCACCTTCTC TCAGCTGTGC 
CAGCAGGTGG ACGTCACCAG ACAGCAGCTG GAGGATGAAA TTGGGGATTT GAACAGCAAG 
ATTGAACAGC TGGACACCCT GCAGAGTAAA GCCAAACTGC TCAGGAATAA GGCGGGCTGG 
CTGGACAGTG AGCTCAACAT GTTCATTCAG CAGTATCTTC AACAGGGGCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mfn2 ( XM_005172484.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172541.1 Danio rerio mitofusin 2 (mfn2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa01083
Clone ID Related Accession (Same CDS sequence)	XM_005172483.4 , XM_005172484.3 , XM_005172484.4 , XM_005172483.3 , NM_001128254.2
Accession Version	XM_005172484.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mitofusin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172484.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGG TTTTCCCGCG ACCCAATACC AGTGTGATCG GCAAGAAGGA CAAGCGACTC 
ATGGCTGAGG TGAACGCATC TCCGCTCAAA CACTTCGTCA CTGCCAAGAA GAAGATCAAC 
GGCATCTTTG AACAGCTGGG CGCCTACATC AAAGAAAGCT CAGCGTTTCT AGAAGAAACC 
TACAGCAATG AAGAGCTGGA TCCTGTTACC ACAGAGGAGC AGGTGGCGGA GGTGCGGGGA 
TACCTGTCCA AAGTGGCAGG GATTGGTGAA GTCCTGTCCC GAAGACACAT GAAAGTGGTC 
TTCTTTGGCA GGACCAGTAA CGGAAAGAGC TCCGTCATTA ATGCCATGCT TTGGGATAAA 
GTGTTGCCGT CTGGAATCGG ACACACCACC AACTGCTTCC TGCGAGTGGA AGGCACTGAT 
GGCAATGAGT CTTTCCTGCT CACTGAAGGG TCGGACGAAA GGAAGAGTGT TAAGACGGTG 
AACCAGTTGG CACATGCTTT ACATCAGGAT GAGGATTTGG ATGCTGGCAG CCTGGTGTGT 
GTCATGTGGC CCAAAGCAAA GTGTGCGCTG CTGCGTGATG ACCTGGTACT TGTGGACAGC 
CCTGGTATTG ATGTCACAAC AGAGCTGGAC AGCTGGATCG ACAAATTCTG CCTCGATGCT 
GACGTTTTTG TGCTGGTGGC CAATTCAGAG TCCACTTTAA TGCAAACAGA AAAATCTTTT 
TTTCACAAAG TAAACGAACG CCTCTCCAGC CCTAATATCT TCATCCTGAA CAACCGCTGG 
GACGCATCGG CCAATGAGCC AGAGTACATG GAAGAGGTGA GGCGGCAGCA CATGGACCGC 
TGCACTAGTT TTTTAGTGGA TGAGCTGCGG GTGGTGGATC GCTCTCATGC CGGGGATCGA 
ATCTTCTTTG TCTCTGCTAA AGAAGTTCTT CAGGCGCGTG TACAGAAGGC CCAGGGAATG 
CCTGAAGCAG GTGGTGCATT AGCTGAAGGT TTTCAAGCCA GAATGTTTGA GTTTCAGAAC 
TTTGAAAGGC GTTTTGAGGA GTGTATCTCG CAGTCAGCAG TGAAGACGAA GTTTGAGCAG 
CACACGACGA GAGCTAAACA GATTTCTGAA GCTCTGCGTC TGATAATGGA TTCTGTGCAC 
ATCGCAGCTC AAGAGCAGAG GATTCATTGC TTGGAGATGA AGGAGGAGAG GGAGGACAGG 
CTGGAGTTCA TTGATAAACA GCTGGATCTG CTCACTCTCG ACTGCAAGAG CAAAATCAAG 
AAGATCACAG AGGAGGTGGA GAAACAGGTT TCCAATGCTA TGGCAGAGGA GATCCGCAGG 
CTGGCGGTAC TGGTGGATGA CTTTCACATG GACTTCCATC CTTCACCAGT CGTGCTCAAA 
GTCTACAAGA ACGAGCTGCA CAGGCACATA GAGGAAGGTC TTGGCAGGAA CATGTCGGAG 
AGATGCTCCA GTGCAATAAC CGTAGCTCTG CAAACCACAC AGACTGACAT GATCGACGGT 
CTGAAGCCAC TGCTGCCTAT CACGATTCGG GAGCAGGTGG ATAAGATGGT GCCCAGACAG 
TGTTTCGCCC TCAGCTACGA CCTCAACTGT GACAAACTCT GCAGCGACTT CCAGGAGGAC 
ATCGGCTTTC ACTTCTCCCT CGGCTGGACG ATGCTGGTCA ACCGCTTCCT GGGCCCCAAA 
AACACACGTC GCGCTCTAAT GGGCTACAAC GACCAGGTTC CCAGATCCCT GGCCATTACT 
CCGGTCAGCA CAAGTATGCC TCCATTCCCT CAAGGATCTA TGACCCAGGA AGAGCTTATG 
GTTTCTATGG TGACAGGACT GGCATCACTC ACCTCTCGGA CATCAATGGG CATCATAGTT 
GTTGGAGGAG TGATCTGGAA AGCAGTGGGC TGGAGGCTCA TTGCTCTGTC TGTGGGTCTC 
TACGGGCTGC TGTACGTGTA TGAGAGACTC ACCTGGACCA CCAGAGCCAA AGAGAGAACA 
TTTAAGAGGC AGTTTGTGGA TTACGCCAGC GAGAAACTGC AGCTCATCGT CAGCTACACC 
GGCTCCAACT GCAGCCATCA AGTTCAGCAG GAGCTGGCCA GCACCTTCTC TCAGCTGTGC 
CAGCAGGTGG ACGTCACCAG ACAGCAGCTG GAGGATGAAA TTGGGGATTT GAACAGCAAG 
ATTGAACAGC TGGACACCCT GCAGAGTAAA GCCAAACTGC TCAGGAATAA GGCGGGCTGG 
CTGGACAGTG AGCTCAACAT GTTCATTCAG CAGTATCTTC AACAGGGGCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172541.1
CDS	288..2561
Translation	MSLVFPRPNTSVIGKKDKRLMAEVNASPLKHFVTAKKKINGIFEQLGAYIKESSAFLEETYSNEELDPVTTEEQVAEVRGYLSKVAGIGEVLSRRHMKVVFFGRTSNGKSSVINAMLWDKVLPSGIGHTTNCFLRVEGTDGNESFLLTEGSDERKSVKTVNQLAHALHQDEDLDAGSLVCVMWPKAKCALLRDDLVLVDSPGIDVTTELDSWIDKFCLDADVFVLVANSESTLMQTEKSFFHKVNERLSSPNIFILNNRWDASANEPEYMEEVRRQHMDRCTSFLVDELRVVDRSHAGDRIFFVSAKEVLQARVQKAQGMPEAGGALAEGFQARMFEFQNFERRFEECISQSAVKTKFEQHTTRAKQISEALRLIMDSVHIAAQEQRIHCLEMKEEREDRLEFIDKQLDLLTLDCKSKIKKITEEVEKQVSNAMAEEIRRLAVLVDDFHMDFHPSPVVLKVYKNELHRHIEEGLGRNMSERCSSAITVALQTTQTDMIDGLKPLLPITIREQVDKMVPRQCFALSYDLNCDKLCSDFQEDIGFHFSLGWTMLVNRFLGPKNTRRALMGYNDQVPRSLAITPVSTSMPPFPQGSMTQEELMVSMVTGLASLTSRTSMGIIVVGGVIWKAVGWRLIALSVGLYGLLYVYERLTWTTRAKERTFKRQFVDYASEKLQLIVSYTGSNCSHQVQQELASTFSQLCQQVDVTRQQLEDEIGDLNSKIEQLDTLQSKAKLLRNKAGWLDSELNMFIQQYLQQGR

Target ORF information:

RefSeq Version	XM_005172484.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mitofusin 2 (mfn2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172484.4

ORF Insert Sequence:

ATGTCTCTGG TTTTCCCGCG ACCCAATACC AGTGTGATCG GCAAGAAGGA CAAGCGACTC 
ATGGCTGAGG TGAACGCATC TCCGCTCAAA CACTTCGTCA CTGCCAAGAA GAAGATCAAC 
GGCATCTTTG AACAGCTGGG CGCCTACATC AAAGAAAGCT CAGCGTTTCT AGAAGAAACC 
TACAGCAATG AAGAGCTGGA TCCTGTTACC ACAGAGGAGC AGGTGGCGGA GGTGCGGGGA 
TACCTGTCCA AAGTGGCAGG GATTGGTGAA GTCCTGTCCC GAAGACACAT GAAAGTGGTC 
TTCTTTGGCA GGACCAGTAA CGGAAAGAGC TCCGTCATTA ATGCCATGCT TTGGGATAAA 
GTGTTGCCGT CTGGAATCGG ACACACCACC AACTGCTTCC TGCGAGTGGA AGGCACTGAT 
GGCAATGAGT CTTTCCTGCT CACTGAAGGG TCGGACGAAA GGAAGAGTGT TAAGACGGTG 
AACCAGTTGG CACATGCTTT ACATCAGGAT GAGGATTTGG ATGCTGGCAG CCTGGTGTGT 
GTCATGTGGC CCAAAGCAAA GTGTGCGCTG CTGCGTGATG ACCTGGTACT TGTGGACAGC 
CCTGGTATTG ATGTCACAAC AGAGCTGGAC AGCTGGATCG ACAAATTCTG CCTCGATGCT 
GACGTTTTTG TGCTGGTGGC CAATTCAGAG TCCACTTTAA TGCAAACAGA AAAATCTTTT 
TTTCACAAAG TAAACGAACG CCTCTCCAGC CCTAATATCT TCATCCTGAA CAACCGCTGG 
GACGCATCGG CCAATGAGCC AGAGTACATG GAAGAGGTGA GGCGGCAGCA CATGGACCGC 
TGCACTAGTT TTTTAGTGGA TGAGCTGCGG GTGGTGGATC GCTCTCATGC CGGGGATCGA 
ATCTTCTTTG TCTCTGCTAA AGAAGTTCTT CAGGCGCGTG TACAGAAGGC CCAGGGAATG 
CCTGAAGCAG GTGGTGCATT AGCTGAAGGT TTTCAAGCCA GAATGTTTGA GTTTCAGAAC 
TTTGAAAGGC GTTTTGAGGA GTGTATCTCG CAGTCAGCAG TGAAGACGAA GTTTGAGCAG 
CACACGACGA GAGCTAAACA GATTTCTGAA GCTCTGCGTC TGATAATGGA TTCTGTGCAC 
ATCGCAGCTC AAGAGCAGAG GATTCATTGC TTGGAGATGA AGGAGGAGAG GGAGGACAGG 
CTGGAGTTCA TTGATAAACA GCTGGATCTG CTCACTCTCG ACTGCAAGAG CAAAATCAAG 
AAGATCACAG AGGAGGTGGA GAAACAGGTT TCCAATGCTA TGGCAGAGGA GATCCGCAGG 
CTGGCGGTAC TGGTGGATGA CTTTCACATG GACTTCCATC CTTCACCAGT CGTGCTCAAA 
GTCTACAAGA ACGAGCTGCA CAGGCACATA GAGGAAGGTC TTGGCAGGAA CATGTCGGAG 
AGATGCTCCA GTGCAATAAC CGTAGCTCTG CAAACCACAC AGACTGACAT GATCGACGGT 
CTGAAGCCAC TGCTGCCTAT CACGATTCGG GAGCAGGTGG ATAAGATGGT GCCCAGACAG 
TGTTTCGCCC TCAGCTACGA CCTCAACTGT GACAAACTCT GCAGCGACTT CCAGGAGGAC 
ATCGGCTTTC ACTTCTCCCT CGGCTGGACG ATGCTGGTCA ACCGCTTCCT GGGCCCCAAA 
AACACACGTC GCGCTCTAAT GGGCTACAAC GACCAGGTTC CCAGATCCCT GGCCATTACT 
CCGGTCAGCA CAAGTATGCC TCCATTCCCT CAAGGATCTA TGACCCAGGA AGAGCTTATG 
GTTTCTATGG TGACAGGACT GGCATCACTC ACCTCTCGGA CATCAATGGG CATCATAGTT 
GTTGGAGGAG TGATCTGGAA AGCAGTGGGC TGGAGGCTCA TTGCTCTGTC TGTGGGTCTC 
TACGGGCTGC TGTACGTGTA TGAGAGACTC ACCTGGACCA CCAGAGCCAA AGAGAGAACA 
TTTAAGAGGC AGTTTGTGGA TTACGCCAGC GAGAAACTGC AGCTCATCGT CAGCTACACC 
GGCTCCAACT GCAGCCATCA AGTTCAGCAG GAGCTGGCCA GCACCTTCTC TCAGCTGTGC 
CAGCAGGTGG ACGTCACCAG ACAGCAGCTG GAGGATGAAA TTGGGGATTT GAACAGCAAG 
ATTGAACAGC TGGACACCCT GCAGAGTAAA GCCAAACTGC TCAGGAATAA GGCGGGCTGG 
CTGGACAGTG AGCTCAACAT GTTCATTCAG CAGTATCTTC AACAGGGGCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mfn2 ( XM_005172483.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172540.1 Danio rerio mitofusin 2 (mfn2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa01083
Clone ID Related Accession (Same CDS sequence)	XM_005172483.4 , XM_005172484.3 , XM_005172484.4 , XM_005172483.3 , NM_001128254.2
Accession Version	XM_005172483.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	mitofusin-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172483.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCTGG TTTTCCCGCG ACCCAATACC AGTGTGATCG GCAAGAAGGA CAAGCGACTC 
ATGGCTGAGG TGAACGCATC TCCGCTCAAA CACTTCGTCA CTGCCAAGAA GAAGATCAAC 
GGCATCTTTG AACAGCTGGG CGCCTACATC AAAGAAAGCT CAGCGTTTCT AGAAGAAACC 
TACAGCAATG AAGAGCTGGA TCCTGTTACC ACAGAGGAGC AGGTGGCGGA GGTGCGGGGA 
TACCTGTCCA AAGTGGCAGG GATTGGTGAA GTCCTGTCCC GAAGACACAT GAAAGTGGTC 
TTCTTTGGCA GGACCAGTAA CGGAAAGAGC TCCGTCATTA ATGCCATGCT TTGGGATAAA 
GTGTTGCCGT CTGGAATCGG ACACACCACC AACTGCTTCC TGCGAGTGGA AGGCACTGAT 
GGCAATGAGT CTTTCCTGCT CACTGAAGGG TCGGACGAAA GGAAGAGTGT TAAGACGGTG 
AACCAGTTGG CACATGCTTT ACATCAGGAT GAGGATTTGG ATGCTGGCAG CCTGGTGTGT 
GTCATGTGGC CCAAAGCAAA GTGTGCGCTG CTGCGTGATG ACCTGGTACT TGTGGACAGC 
CCTGGTATTG ATGTCACAAC AGAGCTGGAC AGCTGGATCG ACAAATTCTG CCTCGATGCT 
GACGTTTTTG TGCTGGTGGC CAATTCAGAG TCCACTTTAA TGCAAACAGA AAAATCTTTT 
TTTCACAAAG TAAACGAACG CCTCTCCAGC CCTAATATCT TCATCCTGAA CAACCGCTGG 
GACGCATCGG CCAATGAGCC AGAGTACATG GAAGAGGTGA GGCGGCAGCA CATGGACCGC 
TGCACTAGTT TTTTAGTGGA TGAGCTGCGG GTGGTGGATC GCTCTCATGC CGGGGATCGA 
ATCTTCTTTG TCTCTGCTAA AGAAGTTCTT CAGGCGCGTG TACAGAAGGC CCAGGGAATG 
CCTGAAGCAG GTGGTGCATT AGCTGAAGGT TTTCAAGCCA GAATGTTTGA GTTTCAGAAC 
TTTGAAAGGC GTTTTGAGGA GTGTATCTCG CAGTCAGCAG TGAAGACGAA GTTTGAGCAG 
CACACGACGA GAGCTAAACA GATTTCTGAA GCTCTGCGTC TGATAATGGA TTCTGTGCAC 
ATCGCAGCTC AAGAGCAGAG GATTCATTGC TTGGAGATGA AGGAGGAGAG GGAGGACAGG 
CTGGAGTTCA TTGATAAACA GCTGGATCTG CTCACTCTCG ACTGCAAGAG CAAAATCAAG 
AAGATCACAG AGGAGGTGGA GAAACAGGTT TCCAATGCTA TGGCAGAGGA GATCCGCAGG 
CTGGCGGTAC TGGTGGATGA CTTTCACATG GACTTCCATC CTTCACCAGT CGTGCTCAAA 
GTCTACAAGA ACGAGCTGCA CAGGCACATA GAGGAAGGTC TTGGCAGGAA CATGTCGGAG 
AGATGCTCCA GTGCAATAAC CGTAGCTCTG CAAACCACAC AGACTGACAT GATCGACGGT 
CTGAAGCCAC TGCTGCCTAT CACGATTCGG GAGCAGGTGG ATAAGATGGT GCCCAGACAG 
TGTTTCGCCC TCAGCTACGA CCTCAACTGT GACAAACTCT GCAGCGACTT CCAGGAGGAC 
ATCGGCTTTC ACTTCTCCCT CGGCTGGACG ATGCTGGTCA ACCGCTTCCT GGGCCCCAAA 
AACACACGTC GCGCTCTAAT GGGCTACAAC GACCAGGTTC CCAGATCCCT GGCCATTACT 
CCGGTCAGCA CAAGTATGCC TCCATTCCCT CAAGGATCTA TGACCCAGGA AGAGCTTATG 
GTTTCTATGG TGACAGGACT GGCATCACTC ACCTCTCGGA CATCAATGGG CATCATAGTT 
GTTGGAGGAG TGATCTGGAA AGCAGTGGGC TGGAGGCTCA TTGCTCTGTC TGTGGGTCTC 
TACGGGCTGC TGTACGTGTA TGAGAGACTC ACCTGGACCA CCAGAGCCAA AGAGAGAACA 
TTTAAGAGGC AGTTTGTGGA TTACGCCAGC GAGAAACTGC AGCTCATCGT CAGCTACACC 
GGCTCCAACT GCAGCCATCA AGTTCAGCAG GAGCTGGCCA GCACCTTCTC TCAGCTGTGC 
CAGCAGGTGG ACGTCACCAG ACAGCAGCTG GAGGATGAAA TTGGGGATTT GAACAGCAAG 
ATTGAACAGC TGGACACCCT GCAGAGTAAA GCCAAACTGC TCAGGAATAA GGCGGGCTGG 
CTGGACAGTG AGCTCAACAT GTTCATTCAG CAGTATCTTC AACAGGGGCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172540.1
CDS	464..2737
Translation	MSLVFPRPNTSVIGKKDKRLMAEVNASPLKHFVTAKKKINGIFEQLGAYIKESSAFLEETYSNEELDPVTTEEQVAEVRGYLSKVAGIGEVLSRRHMKVVFFGRTSNGKSSVINAMLWDKVLPSGIGHTTNCFLRVEGTDGNESFLLTEGSDERKSVKTVNQLAHALHQDEDLDAGSLVCVMWPKAKCALLRDDLVLVDSPGIDVTTELDSWIDKFCLDADVFVLVANSESTLMQTEKSFFHKVNERLSSPNIFILNNRWDASANEPEYMEEVRRQHMDRCTSFLVDELRVVDRSHAGDRIFFVSAKEVLQARVQKAQGMPEAGGALAEGFQARMFEFQNFERRFEECISQSAVKTKFEQHTTRAKQISEALRLIMDSVHIAAQEQRIHCLEMKEEREDRLEFIDKQLDLLTLDCKSKIKKITEEVEKQVSNAMAEEIRRLAVLVDDFHMDFHPSPVVLKVYKNELHRHIEEGLGRNMSERCSSAITVALQTTQTDMIDGLKPLLPITIREQVDKMVPRQCFALSYDLNCDKLCSDFQEDIGFHFSLGWTMLVNRFLGPKNTRRALMGYNDQVPRSLAITPVSTSMPPFPQGSMTQEELMVSMVTGLASLTSRTSMGIIVVGGVIWKAVGWRLIALSVGLYGLLYVYERLTWTTRAKERTFKRQFVDYASEKLQLIVSYTGSNCSHQVQQELASTFSQLCQQVDVTRQQLEDEIGDLNSKIEQLDTLQSKAKLLRNKAGWLDSELNMFIQQYLQQGR

Target ORF information:

RefSeq Version	XM_005172483.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mitofusin 2 (mfn2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172483.3

ORF Insert Sequence:

ATGTCTCTGG TTTTCCCGCG ACCCAATACC AGTGTGATCG GCAAGAAGGA CAAGCGACTC 
ATGGCTGAGG TGAACGCATC TCCGCTCAAA CACTTCGTCA CTGCCAAGAA GAAGATCAAC 
GGCATCTTTG AACAGCTGGG CGCCTACATC AAAGAAAGCT CAGCGTTTCT AGAAGAAACC 
TACAGCAATG AAGAGCTGGA TCCTGTTACC ACAGAGGAGC AGGTGGCGGA GGTGCGGGGA 
TACCTGTCCA AAGTGGCAGG GATTGGTGAA GTCCTGTCCC GAAGACACAT GAAAGTGGTC 
TTCTTTGGCA GGACCAGTAA CGGAAAGAGC TCCGTCATTA ATGCCATGCT TTGGGATAAA 
GTGTTGCCGT CTGGAATCGG ACACACCACC AACTGCTTCC TGCGAGTGGA AGGCACTGAT 
GGCAATGAGT CTTTCCTGCT CACTGAAGGG TCGGACGAAA GGAAGAGTGT TAAGACGGTG 
AACCAGTTGG CACATGCTTT ACATCAGGAT GAGGATTTGG ATGCTGGCAG CCTGGTGTGT 
GTCATGTGGC CCAAAGCAAA GTGTGCGCTG CTGCGTGATG ACCTGGTACT TGTGGACAGC 
CCTGGTATTG ATGTCACAAC AGAGCTGGAC AGCTGGATCG ACAAATTCTG CCTCGATGCT 
GACGTTTTTG TGCTGGTGGC CAATTCAGAG TCCACTTTAA TGCAAACAGA AAAATCTTTT 
TTTCACAAAG TAAACGAACG CCTCTCCAGC CCTAATATCT TCATCCTGAA CAACCGCTGG 
GACGCATCGG CCAATGAGCC AGAGTACATG GAAGAGGTGA GGCGGCAGCA CATGGACCGC 
TGCACTAGTT TTTTAGTGGA TGAGCTGCGG GTGGTGGATC GCTCTCATGC CGGGGATCGA 
ATCTTCTTTG TCTCTGCTAA AGAAGTTCTT CAGGCGCGTG TACAGAAGGC CCAGGGAATG 
CCTGAAGCAG GTGGTGCATT AGCTGAAGGT TTTCAAGCCA GAATGTTTGA GTTTCAGAAC 
TTTGAAAGGC GTTTTGAGGA GTGTATCTCG CAGTCAGCAG TGAAGACGAA GTTTGAGCAG 
CACACGACGA GAGCTAAACA GATTTCTGAA GCTCTGCGTC TGATAATGGA TTCTGTGCAC 
ATCGCAGCTC AAGAGCAGAG GATTCATTGC TTGGAGATGA AGGAGGAGAG GGAGGACAGG 
CTGGAGTTCA TTGATAAACA GCTGGATCTG CTCACTCTCG ACTGCAAGAG CAAAATCAAG 
AAGATCACAG AGGAGGTGGA GAAACAGGTT TCCAATGCTA TGGCAGAGGA GATCCGCAGG 
CTGGCGGTAC TGGTGGATGA CTTTCACATG GACTTCCATC CTTCACCAGT CGTGCTCAAA 
GTCTACAAGA ACGAGCTGCA CAGGCACATA GAGGAAGGTC TTGGCAGGAA CATGTCGGAG 
AGATGCTCCA GTGCAATAAC CGTAGCTCTG CAAACCACAC AGACTGACAT GATCGACGGT 
CTGAAGCCAC TGCTGCCTAT CACGATTCGG GAGCAGGTGG ATAAGATGGT GCCCAGACAG 
TGTTTCGCCC TCAGCTACGA CCTCAACTGT GACAAACTCT GCAGCGACTT CCAGGAGGAC 
ATCGGCTTTC ACTTCTCCCT CGGCTGGACG ATGCTGGTCA ACCGCTTCCT GGGCCCCAAA 
AACACACGTC GCGCTCTAAT GGGCTACAAC GACCAGGTTC CCAGATCCCT GGCCATTACT 
CCGGTCAGCA CAAGTATGCC TCCATTCCCT CAAGGATCTA TGACCCAGGA AGAGCTTATG 
GTTTCTATGG TGACAGGACT GGCATCACTC ACCTCTCGGA CATCAATGGG CATCATAGTT 
GTTGGAGGAG TGATCTGGAA AGCAGTGGGC TGGAGGCTCA TTGCTCTGTC TGTGGGTCTC 
TACGGGCTGC TGTACGTGTA TGAGAGACTC ACCTGGACCA CCAGAGCCAA AGAGAGAACA 
TTTAAGAGGC AGTTTGTGGA TTACGCCAGC GAGAAACTGC AGCTCATCGT CAGCTACACC 
GGCTCCAACT GCAGCCATCA AGTTCAGCAG GAGCTGGCCA GCACCTTCTC TCAGCTGTGC 
CAGCAGGTGG ACGTCACCAG ACAGCAGCTG GAGGATGAAA TTGGGGATTT GAACAGCAAG 
ATTGAACAGC TGGACACCCT GCAGAGTAAA GCCAAACTGC TCAGGAATAA GGCGGGCTGG 
CTGGACAGTG AGCTCAACAT GTTCATTCAG CAGTATCTTC AACAGGGGCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Pla2g6 Deficiency in Zebrafish Leads to Dopaminergic Cell Death, Axonal Degeneration, Increased ?-Synuclein Expression, and Defects in Brain Functions and Pathways.
Molecular neurobiology(2018 Jan)
S?nchez E,Azcona LJ,Pais?n-Ruiz C

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

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mfn2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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