tmc1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tmc1
Entrez Gene ID	567237
Full Name	transmembrane channel-like 1
Synonyms	si:dkey-229d2.1
General protein information	Preferred Names transmembrane channel-like 1 Names transmembrane channel-like protein 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021476287.1	XP_021331962.1	transmembrane channel-like protein 1 isoform X1
NM_001312681.1	NP_001299610.1	transmembrane channel-like protein 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Tmc1	NP_083229.1
Canis lupus familiaris (dog)	TMC1	XP_005616877.1
Bos taurus (cattle)	TMC1	XP_002689695.2
Danio rerio (zebrafish)	tmc1	XP_695621.4
Rattus norvegicus (Norway rat)	Tmc1	NP_001101991.1
Xenopus tropicalis (tropical clawed frog)	tmc1	XP_002935639.1
Homo sapiens (human)	TMC1	NP_619636.2
Pan troglodytes (chimpanzee)	TMC1	XP_528322.2
	TMC1	XP_001093188.2
Gallus gallus (chicken)	TMC1	NP_001006580.1

Gene Ontology
Bibliography

Related articles in PubMed

A molecular basis for water motion detection by the mechanosensory lateral line of zebrafish.
Chou SW, Chen Z, Zhu S, Davis RW, Hu J, Liu L, Fernando CA, Kindig K, Brown WC, Stepanyan R, McDermott BM Jr
Nature communications8(1)2234(2017 Dec)

Integration of Tmc1/2 into the mechanotransduction complex in zebrafish hair cells is regulated by Transmembrane O-methyltransferase (Tomt).
Erickson T, Morgan CP, Olt J, Hardy K, Busch-Nentwich E, Maeda R, Clemens R, Krey JF, Nechiporuk A, Barr-Gillespie PG, Marcotti W, Nicolson T
eLife6(2017 May)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results identify an evolutionarily conserved association between the fish and mouse orthologs of PCDH15 and TMC1 and TMC2, supporting the notion that TMCs are key components of the transduction complex in hair cells.
Title: Tip-link protein protocadherin 15 interacts with transmembrane channel-like proteins TMC1 and TMC2.

The following tmc1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tmc1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa45091	NM_001312681.1 Latest version!	Danio rerio transmembrane channel-like 1 (tmc1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa45092	XM_021476287.1 Latest version!	Danio rerio transmembrane channel-like 1 (tmc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tmc1 ( NM_001312681.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001299610.1 Danio rerio transmembrane channel-like 1 (tmc1), mRNA.

Price & Availability↑

CloneID	ODa45091
Clone ID Related Accession (Same CDS sequence)	NM_001312681.1
Accession Version	NM_001312681.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-10
Organism	Danio rerio(zebrafish)
Product	transmembrane channel-like protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX296526.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: KM115406.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCTAGTAA AAAGTGAACT CTTCATGCAC GCTACTCATC ACCGGCAGGC TCTGTTCAGT 
CAGGCCATGG TCTACTGCTG CGACATCATC ATCATCATCA TCATCATCAT CATCATCATC 
ACGCACACAC ACGGTCAACC ACATGCAAGG CTACTCAGGA GAAGGATGCC ACGGCATAAA 
CTCATCGCAT CTGAAAGTGA CGTGAGCATT GAGGTGGATG AAGGAAAGGA CAAAGAATCC 
TGTGTTTATT ATGTGGAAGT GGAGGAAAAT TGTGAGAGAG GCAAGATAAA GCAGGCTAGT 
CGAGATGGCA AACGAAGGAG GGAGAGAAAT GGTGAAACTA GGAGAAAAGC TTCAGAGAAA 
AGAACAAACG AGGGGGAAAG TAAAAAAGCA GAAAAGAAAC ATGAGAAAGG CCACAGAACT 
GCAAGGAAAG CAGGAGAGAA ACATGGAAAA AGGCAAAGAC GAAAGAATGC AGGAGAAGAG 
GATGCTGAGG ATAAATCCAG CAAAGAGAAA AAGAACATGA AAAATGAAAA AAACAAGACT 
TTAAAACTGG AGGAGGAGAA GGAGAAGGAT GTGAGGAAAA AGAAAAGGAA ACATGTTAAA 
AATGAAGAGG ACGAGACAAA TCATGAAAAG ACAAAACAGC ATTTGAAGGA AGAAAAGAGA 
AGAAAGAAAA GAAAGAAGCC AGAGACTACC TCAGAGTCGG AGTCTAAATC TGAGTCTGAA 
TCTGCTTCTG AATCTGAGTC AAAGAACAGT CCGGCAGTGG GAGTTTTGGG TTCTCTGACT 
CCAGAGGAGC TGGAAAATTT AAAAGAAGCT GTGGAGGAAA GAAAAAAGCT CATCACACAA 
CTAAAAGGAA AACCCTGGCC CATGAGAAGG AAACTAGTGG TCCTCAGGGA GTCGCAGGAG 
TTTGTGGAGA AATATGAAGG TGCTTTGGGG AAAGGCAAAG GCAGGAAACT TTATGCCTAC 
AAGGTCATGA TGATGAAGAA ATGGATGAAG TTTCAGCGTG ATTTTGAGAA CTTCAAGACT 
GCCTGCATCC CGTGGGAGAT GAAGATAAAA GAAATCGAAA GCCATTTTGG CTCTTCGGTG 
GCTTCATACT TCATATTCTT GAGGTGGATG TATGGCATCA ATATGATTTT GTTTGGACTG 
ACATTTGGGC TGGTCATGGT TCCAGAGGCT CTTATGGGAA AACCATATGG CAGTTTACCA 
AGGAAAACAG TGCCCAGAGA GGAAGAGGCC AGTGCTATGA ATTTTGCAGT CTTATGGGAT 
TTTGGGGGCT ATGCTAAGTA CTCTGTCCTC TTCTATGGCT ACTATAACAG CCAGCGGGCC 
ATTGGGTGGC TGAAGTTTCG CATGCCACTG TCCTACTTCC TGGTTGGAGT GGGCACAGTG 
GCCTACAGCT ACATGGTGGT GATCAGGACC ATGGCTCGCA ACGCAAATGA AGAAGGTGGC 
GGAGATGATA CCAGCTTTAA TTTCAGCTGG AAGACATTCA CAAGCTGGGA TTACCTTATT 
GGAAACCCTG AGACAGCAGA CAACAAGTTT GCTTCTATCA CCACCAGCTT TAAGGAGGCT 
ATTGTGGAGG AGCAAGAGAG CAGAAAAGAT GACAACATCC ACCTGACAAG ATTCCTCAGA 
GTGCTGGCCA ACTTCCTGGT GCTCTGCTGT CTGGCAGGGA GCGGGTACTT GATCTACTTT 
GTAGTACGCA GGTCTCAGAA GTTTGCACTG GAGGGCCTGG AGAACTACGG CTGGTGGGAG 
AGGAATGAGG TCAACATGGT GATGTCTCTG TTGGGAATGT TCTGCCCGAT GCTGTTCGAT 
GTGATCAGCA CTTTGGAGAA CTATCACCCA CGCATTGCCC TCCAGTGGCA GCTGGGACGC 
ATCTTTGCTC TTTTTTTGGG GAACCTGTAC ACATTTATCA TCGCCCTCAT GGATGCCATC 
CAACTAAAGA GGGCAGAAGA GGAGATTGTG AAGAAGAATA TGACAATCTG GCAGGCCAAC 
TTGTACAATG GGACTGTGCC AGACAACTCG ACTGCTCCTC CGCTCACTGT GCACCCTGCT 
GATGTTCCCA GAGGGCCTTG CTGGGAGACC ATGGTGGGAC AGGAGTTTGT TCGTCTTATC 
ATTTCTGACA CAATGACGAC TTACATCACT CTGCTGATTG GGGATTTTAT GCGTGCCGTA 
CTGGTGCGAT TCCTGAACAA CTGCTGGTGC TGGGATCTGG AGTATGGCTT TCCATCCTAC 
TCAGAGTTTG ATGTTAGTGG GAATGTTCTG GGGCTGATCT TTAACCAGGG AATGATCTGG 
ATGGGTGCAT TCTACGCTCC ATGTCTTCCA GCACTAAATC TTCTTCGTCT TCACGTGTCT 
ATGTACCTGC AGTGTTGGGC AGTGATGTGC TGTAATGTGC CTCAGGAACG CGTCTTCAAA 
GCTTCAGGCT CCAATAACTT CTACATGGCC ATGCTGCTGG TCATTCTTTT TCTCTCTACA 
TTACCAGCCA TATACACCAT TGTCTCCATT CCTCCCTCCT TCGACTGCGG GCCCTTCAGT 
GGGAAACCCC GCATGTTTGA TGTGATCCAG GAGACTCTGG AGACAGATTT CCCTGCCTGG 
TTCAGTAAAG TGTTCAGTTA TGCCTCAAAC CCCGGTCTGG TGCTGCCGTT CCTTCTTCTT 
TTGGTTCTCG CAATATATTA CCTGCAGTCC ACTTCGAAAA CGTATAAAAG AGTAAACATG 
GAGCTGAAAA AGAAACTTCA AGCACAAAAT GAGGAAAACA AGAAAAAGAA CAAACTTGCA 
GCCTTAAAAG CAGCCAGTGA CTTGGAGCAG GCAAGAAAAG CTGGAGAGCA ACGCAGAAAC 
AGCATCTCAG ATTTGGGAGT GAATGAAGAG AATCCTGAAT CTCACGTTTC CTCATCCCAC 
ACATCCAGAC CCCCTGCGTC CCGTGGCCAC ACATCATCTG GACATCTTCC AGGTCACCCG 
CAGCAGCCAC AGAAAAATTC GAAGAAGAGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001299610.1
CDS	46..3018
Translation	MLVKSELFMHATHHRQALFSQAMVYCCDIIIIIIIIIIIITHTHGQPHARLLRRRMPRHKLIASESDVSIEVDEGKDKESCVYYVEVEENCERGKIKQASRDGKRRRERNGETRRKASEKRTNEGESKKAEKKHEKGHRTARKAGEKHGKRQRRKNAGEEDAEDKSSKEKKNMKNEKNKTLKLEEEKEKDVRKKKRKHVKNEEDETNHEKTKQHLKEEKRRKKRKKPETTSESESKSESESASESESKNSPAVGVLGSLTPEELENLKEAVEERKKLITQLKGKPWPMRRKLVVLRESQEFVEKYEGALGKGKGRKLYAYKVMMMKKWMKFQRDFENFKTACIPWEMKIKEIESHFGSSVASYFIFLRWMYGINMILFGLTFGLVMVPEALMGKPYGSLPRKTVPREEEASAMNFAVLWDFGGYAKYSVLFYGYYNSQRAIGWLKFRMPLSYFLVGVGTVAYSYMVVIRTMARNANEEGGGDDTSFNFSWKTFTSWDYLIGNPETADNKFASITTSFKEAIVEEQESRKDDNIHLTRFLRVLANFLVLCCLAGSGYLIYFVVRRSQKFALEGLENYGWWERNEVNMVMSLLGMFCPMLFDVISTLENYHPRIALQWQLGRIFALFLGNLYTFIIALMDAIQLKRAEEEIVKKNMTIWQANLYNGTVPDNSTAPPLTVHPADVPRGPCWETMVGQEFVRLIISDTMTTYITLLIGDFMRAVLVRFLNNCWCWDLEYGFPSYSEFDVSGNVLGLIFNQGMIWMGAFYAPCLPALNLLRLHVSMYLQCWAVMCCNVPQERVFKASGSNNFYMAMLLVILFLSTLPAIYTIVSIPPSFDCGPFSGKPRMFDVIQETLETDFPAWFSKVFSYASNPGLVLPFLLLLVLAIYYLQSTSKTYKRVNMELKKKLQAQNEENKKKNKLAALKAASDLEQARKAGEQRRNSISDLGVNEENPESHVSSSHTSRPPASRGHTSSGHLPGHPQQPQKNSKKR

Target ORF information:

RefSeq Version	NM_001312681.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane channel-like 1 (tmc1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001312681.1

ORF Insert Sequence:

ATGCTAGTAA AAAGTGAACT CTTCATGCAC GCTACTCATC ACCGGCAGGC TCTGTTCAGT 
CAGGCCATGG TCTACTGCTG CGACATCATC ATCATCATCA TCATCATCAT CATCATCATC 
ACGCACACAC ACGGTCAACC ACATGCAAGG CTACTCAGGA GAAGGATGCC ACGGCATAAA 
CTCATCGCAT CTGAAAGTGA CGTGAGCATT GAGGTGGATG AAGGAAAGGA CAAAGAATCC 
TGTGTTTATT ATGTGGAAGT GGAGGAAAAT TGTGAGAGAG GCAAGATAAA GCAGGCTAGT 
CGAGATGGCA AACGAAGGAG GGAGAGAAAT GGTGAAACTA GGAGAAAAGC TTCAGAGAAA 
AGAACAAACG AGGGGGAAAG TAAAAAAGCA GAAAAGAAAC ATGAGAAAGG CCACAGAACT 
GCAAGGAAAG CAGGAGAGAA ACATGGAAAA AGGCAAAGAC GAAAGAATGC AGGAGAAGAG 
GATGCTGAGG ATAAATCCAG CAAAGAGAAA AAGAACATGA AAAATGAAAA AAACAAGACT 
TTAAAACTGG AGGAGGAGAA GGAGAAGGAT GTGAGGAAAA AGAAAAGGAA ACATGTTAAA 
AATGAAGAGG ACGAGACAAA TCATGAAAAG ACAAAACAGC ATTTGAAGGA AGAAAAGAGA 
AGAAAGAAAA GAAAGAAGCC AGAGACTACC TCAGAGTCGG AGTCTAAATC TGAGTCTGAA 
TCTGCTTCTG AATCTGAGTC AAAGAACAGT CCGGCAGTGG GAGTTTTGGG TTCTCTGACT 
CCAGAGGAGC TGGAAAATTT AAAAGAAGCT GTGGAGGAAA GAAAAAAGCT CATCACACAA 
CTAAAAGGAA AACCCTGGCC CATGAGAAGG AAACTAGTGG TCCTCAGGGA GTCGCAGGAG 
TTTGTGGAGA AATATGAAGG TGCTTTGGGG AAAGGCAAAG GCAGGAAACT TTATGCCTAC 
AAGGTCATGA TGATGAAGAA ATGGATGAAG TTTCAGCGTG ATTTTGAGAA CTTCAAGACT 
GCCTGCATCC CGTGGGAGAT GAAGATAAAA GAAATCGAAA GCCATTTTGG CTCTTCGGTG 
GCTTCATACT TCATATTCTT GAGGTGGATG TATGGCATCA ATATGATTTT GTTTGGACTG 
ACATTTGGGC TGGTCATGGT TCCAGAGGCT CTTATGGGAA AACCATATGG CAGTTTACCA 
AGGAAAACAG TGCCCAGAGA GGAAGAGGCC AGTGCTATGA ATTTTGCAGT CTTATGGGAT 
TTTGGGGGCT ATGCTAAGTA CTCTGTCCTC TTCTATGGCT ACTATAACAG CCAGCGGGCC 
ATTGGGTGGC TGAAGTTTCG CATGCCACTG TCCTACTTCC TGGTTGGAGT GGGCACAGTG 
GCCTACAGCT ACATGGTGGT GATCAGGACC ATGGCTCGCA ACGCAAATGA AGAAGGTGGC 
GGAGATGATA CCAGCTTTAA TTTCAGCTGG AAGACATTCA CAAGCTGGGA TTACCTTATT 
GGAAACCCTG AGACAGCAGA CAACAAGTTT GCTTCTATCA CCACCAGCTT TAAGGAGGCT 
ATTGTGGAGG AGCAAGAGAG CAGAAAAGAT GACAACATCC ACCTGACAAG ATTCCTCAGA 
GTGCTGGCCA ACTTCCTGGT GCTCTGCTGT CTGGCAGGGA GCGGGTACTT GATCTACTTT 
GTAGTACGCA GGTCTCAGAA GTTTGCACTG GAGGGCCTGG AGAACTACGG CTGGTGGGAG 
AGGAATGAGG TCAACATGGT GATGTCTCTG TTGGGAATGT TCTGCCCGAT GCTGTTCGAT 
GTGATCAGCA CTTTGGAGAA CTATCACCCA CGCATTGCCC TCCAGTGGCA GCTGGGACGC 
ATCTTTGCTC TTTTTTTGGG GAACCTGTAC ACATTTATCA TCGCCCTCAT GGATGCCATC 
CAACTAAAGA GGGCAGAAGA GGAGATTGTG AAGAAGAATA TGACAATCTG GCAGGCCAAC 
TTGTACAATG GGACTGTGCC AGACAACTCG ACTGCTCCTC CGCTCACTGT GCACCCTGCT 
GATGTTCCCA GAGGGCCTTG CTGGGAGACC ATGGTGGGAC AGGAGTTTGT TCGTCTTATC 
ATTTCTGACA CAATGACGAC TTACATCACT CTGCTGATTG GGGATTTTAT GCGTGCCGTA 
CTGGTGCGAT TCCTGAACAA CTGCTGGTGC TGGGATCTGG AGTATGGCTT TCCATCCTAC 
TCAGAGTTTG ATGTTAGTGG GAATGTTCTG GGGCTGATCT TTAACCAGGG AATGATCTGG 
ATGGGTGCAT TCTACGCTCC ATGTCTTCCA GCACTAAATC TTCTTCGTCT TCACGTGTCT 
ATGTACCTGC AGTGTTGGGC AGTGATGTGC TGTAATGTGC CTCAGGAACG CGTCTTCAAA 
GCTTCAGGCT CCAATAACTT CTACATGGCC ATGCTGCTGG TCATTCTTTT TCTCTCTACA 
TTACCAGCCA TATACACCAT TGTCTCCATT CCTCCCTCCT TCGACTGCGG GCCCTTCAGT 
GGGAAACCCC GCATGTTTGA TGTGATCCAG GAGACTCTGG AGACAGATTT CCCTGCCTGG 
TTCAGTAAAG TGTTCAGTTA TGCCTCAAAC CCCGGTCTGG TGCTGCCGTT CCTTCTTCTT 
TTGGTTCTCG CAATATATTA CCTGCAGTCC ACTTCGAAAA CGTATAAAAG AGTAAACATG 
GAGCTGAAAA AGAAACTTCA AGCACAAAAT GAGGAAAACA AGAAAAAGAA CAAACTTGCA 
GCCTTAAAAG CAGCCAGTGA CTTGGAGCAG GCAAGAAAAG CTGGAGAGCA ACGCAGAAAC 
AGCATCTCAG ATTTGGGAGT GAATGAAGAG AATCCTGAAT CTCACGTTTC CTCATCCCAC 
ACATCCAGAC CCCCTGCGTC CCGTGGCCAC ACATCATCTG GACATCTTCC AGGTCACCCG 
CAGCAGCCAC AGAAAAATTC GAAGAAGAGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tmc1 ( XM_021476287.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331962.1 Danio rerio transmembrane channel-like 1 (tmc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa45092
Clone ID Related Accession (Same CDS sequence)	XM_021476287.1
Accession Version	XM_021476287.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2973bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transmembrane channel-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACGT TTAATAGGAA AGAGAATCAT CAAGAAGACT TGTTCCTGGC TCTGTTCAGT 
CAGGCCATGG TCTACTGCTG CGACATCATC ATCATCATCA TCATCATCAT CATCATCATC 
ACGCACACAC ACGGTCAACC ACATGCAAGG CTACTCAGGA GAAGGATGCC ACGGCATAAA 
CTCATCGCAT CTGAAAGTGA CGTGAGCATT GAGGTGGATG AAGGAAAGGA CAAAGAATCC 
TGTGTTTATT ATGTGGAAGT GGAGGAAAAT TGTGAGAGAG GCAAGATAAA GCAGGCTAGT 
CGAGATGGCA AACGAAGGAG GGAGAGAAAT GGTGAAACTA GGAGAAAAGC TTCAGAGAAA 
AGAACAAACG AGGGGGAAAG TAAAAAAGCA GAAAAGAAAC ATGAGAAAGG CCACAGAACT 
GCAAGGAAAG CAGGAGAGAA ACATGGAAAA AGGCAAAGAC GAAAGAATGC AGGAGAAGAG 
GATGCTGAGG ATAAATCCAG CAAAGAGAAA AAGAACATGA AAAATGAAAA AAACAAGACT 
TTAAAACTGG AGGAGGAGAA GGAGAAGGAT GTGAGGAAAA AGAAAAGGAA ACATGTTAAA 
AATGAAGAGG ACGAGACAAA TCATGAAAAG ACAAAACAGC ATTTGAAGGA AGAAAAGAGA 
AGAAAGAAAA GAAAGAAGCC AGAGACTACC TCAGAGTCGG AGTCTAAATC TGAGTCTGAA 
TCTGCTTCTG AATCTGAGTC AAAGAACAGT CCGGCAGTGG GAGTTTTGGG TTCTCTGACT 
CCAGAGGAGC TGGAAAATTT AAAAGAAGCT GTGGAGGAAA GAAAAAAGCT CATCACACAA 
CTAAAAGGAA AACCCTGGCC CATGAGAAGG AAACTAGTGG TCCTCAGGGA GTCGCAGGAG 
TTTGTGGAGA AATATGAAGG TGCTTTGGGG AAAGGCAAAG GCAGGAAACT TTATGCCTAC 
AAGGTCATGA TGATGAAGAA ATGGATGAAG TTTCAGCGTG ATTTTGAGAA CTTCAAGACT 
GCCTGCATCC CGTGGGAGAT GAAGATAAAA GAAATCGAAA GCCATTTTGG CTCTTCGGTG 
GCTTCATACT TCATATTCTT GAGGTGGATG TATGGCATCA ATATGATTTT GTTTGGACTG 
ACATTTGGGC TGGTCATGGT TCCAGAGGCT CTTATGGGAA AACCATATGG CAGTTTACCA 
AGGAAAACAG TGCCCAGAGA GGAAGAGGCC AGTGCTATGA ATTTTGCAGT CTTATGGGAT 
TTTGGGGGCT ATGCTAAGTA CTCTGTCCTC TTCTATGGCT ACTATAACAG CCAGCGGGCC 
ATTGGGTGGC TGAAGTTTCG CATGCCACTG TCCTACTTCC TGGTTGGAGT GGGCACAGTG 
GCCTACAGCT ACATGGTGGT GATCAGGACC ATGGCTCGCA ACGCAAATGA AGAAGGTGGC 
GGAGATGATA CCAGCTTTAA TTTCAGCTGG AAGACATTCA CAAGCTGGGA TTACCTTATT 
GGAAACCCTG AGACAGCAGA CAACAAGTTT GCTTCTATCA CCACCAGCTT TAAGGAGGCT 
ATTGTGGAGG AGCAAGAGAG CAGAAAAGAT GACAACATCC ACCTGACAAG ATTCCTCAGA 
GTGCTGGCCA ACTTCCTGGT GCTCTGCTGT CTGGCAGGGA GCGGGTACTT GATCTACTTT 
GTAGTACGCA GGTCTCAGAA GTTTGCACTG GAGGGCCTGG AGAACTACGG CTGGTGGGAG 
AGGAATGAGG TCAACATGGT GATGTCTCTG TTGGGAATGT TCTGCCCGAT GCTGTTCGAT 
GTGATCAGCA CTTTGGAGAA CTATCACCCA CGCATTGCCC TCCAGTGGCA GCTGGGACGC 
ATCTTTGCTC TTTTTTTGGG GAACCTGTAC ACATTTATCA TCGCCCTCAT GGATGCCATC 
CAACTAAAGA GGGCAGAAGA GGAGATTGTG AAGAAGAATA TGACAATCTG GCAGGCCAAC 
TTGTACAATG GGACTGTGCC AGACAACTCG ACTGCTCCTC CGCTCACTGT GCACCCTGCT 
GATGTTCCCA GAGGGCCTTG CTGGGAGACC ATGGTGGGAC AGGAGTTTGT TCGTCTTATC 
ATTTCTGACA CAATGACGAC TTACATCACT CTGCTGATTG GGGATTTTAT GCGTGCCGTA 
CTGGTGCGAT TCCTGAACAA CTGCTGGTGC TGGGATCTGG AGTATGGCTT TCCATCCTAC 
TCAGAGTTTG ATGTTAGTGG GAATGTTCTG GGGCTGATCT TTAACCAGGG AATGATCTGG 
ATGGGTGCAT TCTACGCTCC ATGTCTTCCA GCACTAAATC TTCTTCGTCT TCACGTGTCT 
ATGTACCTGC AGTGTTGGGC AGTGATGTGC TGTAATGTGC CTCAGGAACG CGTCTTCAAA 
GCTTCAGGCT CCAATAACTT CTACATGGCC ATGCTGCTGG TCATTCTTTT TCTCTCTACA 
TTACCAGCCA TATACACCAT TGTCTCCATT CCTCCCTCCT TCGACTGCGG GCCCTTCAGT 
GGGAAACCCC GCATGTTTGA TGTGATCCAG GAGACTCTGG AGACAGATTT CCCTGCCTGG 
TTCAGTAAAG TGTTCAGTTA TGCCTCAAAC CCCGGTCTGG TGCTGCCGTT CCTTCTTCTT 
TTGGTTCTCG CAATATATTA CCTGCAGTCC ACTTCGAAAA CGTATAAAAG AGTAAACATG 
GAGCTGAAAA AGAAACTTCA AGCACAAAAT GAGGAAAACA AGAAAAAGAA CAAACTTGCA 
GCCTTAAAAG CAGCCAGTGA CTTGGAGCAG GCAAGAAAAG CTGGAGAGCA ACGCAGAAAC 
AGCATCTCAG ATTTGGGAGT GAATGAAGAG AATCCTGAAT CTCACGTTTC CTCATCCCAC 
ACATCCAGAC CCCCTGCGTC CCGTGGCCAC ACATCATCTG GACATCTTCC AGGTCACCCG 
CAGCAGCCAC AGAAAAATTC GAAGAAGAGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331962.1
CDS	163..3135
Translation	METFNRKENHQEDLFLALFSQAMVYCCDIIIIIIIIIIIITHTHGQPHARLLRRRMPRHKLIASESDVSIEVDEGKDKESCVYYVEVEENCERGKIKQASRDGKRRRERNGETRRKASEKRTNEGESKKAEKKHEKGHRTARKAGEKHGKRQRRKNAGEEDAEDKSSKEKKNMKNEKNKTLKLEEEKEKDVRKKKRKHVKNEEDETNHEKTKQHLKEEKRRKKRKKPETTSESESKSESESASESESKNSPAVGVLGSLTPEELENLKEAVEERKKLITQLKGKPWPMRRKLVVLRESQEFVEKYEGALGKGKGRKLYAYKVMMMKKWMKFQRDFENFKTACIPWEMKIKEIESHFGSSVASYFIFLRWMYGINMILFGLTFGLVMVPEALMGKPYGSLPRKTVPREEEASAMNFAVLWDFGGYAKYSVLFYGYYNSQRAIGWLKFRMPLSYFLVGVGTVAYSYMVVIRTMARNANEEGGGDDTSFNFSWKTFTSWDYLIGNPETADNKFASITTSFKEAIVEEQESRKDDNIHLTRFLRVLANFLVLCCLAGSGYLIYFVVRRSQKFALEGLENYGWWERNEVNMVMSLLGMFCPMLFDVISTLENYHPRIALQWQLGRIFALFLGNLYTFIIALMDAIQLKRAEEEIVKKNMTIWQANLYNGTVPDNSTAPPLTVHPADVPRGPCWETMVGQEFVRLIISDTMTTYITLLIGDFMRAVLVRFLNNCWCWDLEYGFPSYSEFDVSGNVLGLIFNQGMIWMGAFYAPCLPALNLLRLHVSMYLQCWAVMCCNVPQERVFKASGSNNFYMAMLLVILFLSTLPAIYTIVSIPPSFDCGPFSGKPRMFDVIQETLETDFPAWFSKVFSYASNPGLVLPFLLLLVLAIYYLQSTSKTYKRVNMELKKKLQAQNEENKKKNKLAALKAASDLEQARKAGEQRRNSISDLGVNEENPESHVSSSHTSRPPASRGHTSSGHLPGHPQQPQKNSKKR

Target ORF information:

RefSeq Version	XM_021476287.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transmembrane channel-like 1 (tmc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476287.1

ORF Insert Sequence:

ATGGAGACGT TTAATAGGAA AGAGAATCAT CAAGAAGACT TGTTCCTGGC TCTGTTCAGT 
CAGGCCATGG TCTACTGCTG CGACATCATC ATCATCATCA TCATCATCAT CATCATCATC 
ACGCACACAC ACGGTCAACC ACATGCAAGG CTACTCAGGA GAAGGATGCC ACGGCATAAA 
CTCATCGCAT CTGAAAGTGA CGTGAGCATT GAGGTGGATG AAGGAAAGGA CAAAGAATCC 
TGTGTTTATT ATGTGGAAGT GGAGGAAAAT TGTGAGAGAG GCAAGATAAA GCAGGCTAGT 
CGAGATGGCA AACGAAGGAG GGAGAGAAAT GGTGAAACTA GGAGAAAAGC TTCAGAGAAA 
AGAACAAACG AGGGGGAAAG TAAAAAAGCA GAAAAGAAAC ATGAGAAAGG CCACAGAACT 
GCAAGGAAAG CAGGAGAGAA ACATGGAAAA AGGCAAAGAC GAAAGAATGC AGGAGAAGAG 
GATGCTGAGG ATAAATCCAG CAAAGAGAAA AAGAACATGA AAAATGAAAA AAACAAGACT 
TTAAAACTGG AGGAGGAGAA GGAGAAGGAT GTGAGGAAAA AGAAAAGGAA ACATGTTAAA 
AATGAAGAGG ACGAGACAAA TCATGAAAAG ACAAAACAGC ATTTGAAGGA AGAAAAGAGA 
AGAAAGAAAA GAAAGAAGCC AGAGACTACC TCAGAGTCGG AGTCTAAATC TGAGTCTGAA 
TCTGCTTCTG AATCTGAGTC AAAGAACAGT CCGGCAGTGG GAGTTTTGGG TTCTCTGACT 
CCAGAGGAGC TGGAAAATTT AAAAGAAGCT GTGGAGGAAA GAAAAAAGCT CATCACACAA 
CTAAAAGGAA AACCCTGGCC CATGAGAAGG AAACTAGTGG TCCTCAGGGA GTCGCAGGAG 
TTTGTGGAGA AATATGAAGG TGCTTTGGGG AAAGGCAAAG GCAGGAAACT TTATGCCTAC 
AAGGTCATGA TGATGAAGAA ATGGATGAAG TTTCAGCGTG ATTTTGAGAA CTTCAAGACT 
GCCTGCATCC CGTGGGAGAT GAAGATAAAA GAAATCGAAA GCCATTTTGG CTCTTCGGTG 
GCTTCATACT TCATATTCTT GAGGTGGATG TATGGCATCA ATATGATTTT GTTTGGACTG 
ACATTTGGGC TGGTCATGGT TCCAGAGGCT CTTATGGGAA AACCATATGG CAGTTTACCA 
AGGAAAACAG TGCCCAGAGA GGAAGAGGCC AGTGCTATGA ATTTTGCAGT CTTATGGGAT 
TTTGGGGGCT ATGCTAAGTA CTCTGTCCTC TTCTATGGCT ACTATAACAG CCAGCGGGCC 
ATTGGGTGGC TGAAGTTTCG CATGCCACTG TCCTACTTCC TGGTTGGAGT GGGCACAGTG 
GCCTACAGCT ACATGGTGGT GATCAGGACC ATGGCTCGCA ACGCAAATGA AGAAGGTGGC 
GGAGATGATA CCAGCTTTAA TTTCAGCTGG AAGACATTCA CAAGCTGGGA TTACCTTATT 
GGAAACCCTG AGACAGCAGA CAACAAGTTT GCTTCTATCA CCACCAGCTT TAAGGAGGCT 
ATTGTGGAGG AGCAAGAGAG CAGAAAAGAT GACAACATCC ACCTGACAAG ATTCCTCAGA 
GTGCTGGCCA ACTTCCTGGT GCTCTGCTGT CTGGCAGGGA GCGGGTACTT GATCTACTTT 
GTAGTACGCA GGTCTCAGAA GTTTGCACTG GAGGGCCTGG AGAACTACGG CTGGTGGGAG 
AGGAATGAGG TCAACATGGT GATGTCTCTG TTGGGAATGT TCTGCCCGAT GCTGTTCGAT 
GTGATCAGCA CTTTGGAGAA CTATCACCCA CGCATTGCCC TCCAGTGGCA GCTGGGACGC 
ATCTTTGCTC TTTTTTTGGG GAACCTGTAC ACATTTATCA TCGCCCTCAT GGATGCCATC 
CAACTAAAGA GGGCAGAAGA GGAGATTGTG AAGAAGAATA TGACAATCTG GCAGGCCAAC 
TTGTACAATG GGACTGTGCC AGACAACTCG ACTGCTCCTC CGCTCACTGT GCACCCTGCT 
GATGTTCCCA GAGGGCCTTG CTGGGAGACC ATGGTGGGAC AGGAGTTTGT TCGTCTTATC 
ATTTCTGACA CAATGACGAC TTACATCACT CTGCTGATTG GGGATTTTAT GCGTGCCGTA 
CTGGTGCGAT TCCTGAACAA CTGCTGGTGC TGGGATCTGG AGTATGGCTT TCCATCCTAC 
TCAGAGTTTG ATGTTAGTGG GAATGTTCTG GGGCTGATCT TTAACCAGGG AATGATCTGG 
ATGGGTGCAT TCTACGCTCC ATGTCTTCCA GCACTAAATC TTCTTCGTCT TCACGTGTCT 
ATGTACCTGC AGTGTTGGGC AGTGATGTGC TGTAATGTGC CTCAGGAACG CGTCTTCAAA 
GCTTCAGGCT CCAATAACTT CTACATGGCC ATGCTGCTGG TCATTCTTTT TCTCTCTACA 
TTACCAGCCA TATACACCAT TGTCTCCATT CCTCCCTCCT TCGACTGCGG GCCCTTCAGT 
GGGAAACCCC GCATGTTTGA TGTGATCCAG GAGACTCTGG AGACAGATTT CCCTGCCTGG 
TTCAGTAAAG TGTTCAGTTA TGCCTCAAAC CCCGGTCTGG TGCTGCCGTT CCTTCTTCTT 
TTGGTTCTCG CAATATATTA CCTGCAGTCC ACTTCGAAAA CGTATAAAAG AGTAAACATG 
GAGCTGAAAA AGAAACTTCA AGCACAAAAT GAGGAAAACA AGAAAAAGAA CAAACTTGCA 
GCCTTAAAAG CAGCCAGTGA CTTGGAGCAG GCAAGAAAAG CTGGAGAGCA ACGCAGAAAC 
AGCATCTCAG ATTTGGGAGT GAATGAAGAG AATCCTGAAT CTCACGTTTC CTCATCCCAC 
ACATCCAGAC CCCCTGCGTC CCGTGGCCAC ACATCATCTG GACATCTTCC AGGTCACCCG 
CAGCAGCCAC AGAAAAATTC GAAGAAGAGA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A molecular basis for water motion detection by the mechanosensory lateral line of zebrafish.
Nature communications8(1)2234(2017 Dec)
Chou SW,Chen Z,Zhu S,Davis RW,Hu J,Liu L,Fernando CA,Kindig K,Brown WC,Stepanyan R,McDermott BM Jr

Integration of Tmc1/2 into the mechanotransduction complex in zebrafish hair cells is regulated by Transmembrane O-methyltransferase (Tomt).
eLife6(2017 May)
Erickson T,Morgan CP,Olt J,Hardy K,Busch-Nentwich E,Maeda R,Clemens R,Krey JF,Nechiporuk A,Barr-Gillespie PG,Marcotti W,Nicolson T

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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tmc1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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