ppfia3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ppfia3
Entrez Gene ID	567230
Full Name	protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3
General protein information	Preferred Names protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3 Names liprin-alpha-3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_003198113.4	XP_003198161.2	liprin-alpha-3
XM_003198113.5	XP_003198161.2	liprin-alpha-3 isoform X1
XM_021474035.1	XP_021329710.1	liprin-alpha-3 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PPFIA3	XP_005219545.1
Pan troglodytes (chimpanzee)	PPFIA3	XP_513009.3
Homo sapiens (human)	PPFIA3	NP_003651.1
Mus musculus (house mouse)	Ppfia3	NP_084017.2
Danio rerio (zebrafish)	ppfia3	XP_005164030.1
Xenopus tropicalis (tropical clawed frog)	ppfia3	XP_002934675.2
Canis lupus familiaris (dog)	PPFIA3	XP_005616919.1
Rattus norvegicus (Norway rat)	Ppfia3	NP_001257914.1

Gene Ontology

The following ppfia3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ppfia3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa141763	XM_021474035.1 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3 (ppfia3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa08389	XM_003198113.5 Latest version!	Danio rerio protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3 (ppfia3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa08389	XM_003198113.4	Danio rerio protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3 (ppfia3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ppfia3 ( XM_021474035.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329710.1 Danio rerio protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3 (ppfia3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa141763
Clone ID Related Accession (Same CDS sequence)	XM_021474035.1
Accession Version	XM_021474035.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	liprin-alpha-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC CACCATCTCC GAGGACGGGC GGGGTGGTGC TGGAGGTGGC 
CCGTCCTCTC CTGCTGGAGG TGGTGGGGGT ATGGGGGGCG GTGCAGGGGG CATGGGTGGT 
GGCTATGGTG GCAGGGAGGC CCGCGGCGGA GATGAAGGAG GGAGCACTGG AAACCTGGAG 
TCTCTGATGG TGAACATGCT GACGGAGAGA GAGCGGCTGC TGGAGAGTCT CCGAGAGACC 
CAGGAGAGTC TCGGCACGGC ACAGCTACGT CTGCGAGAGC TGGGTCACGA GAAGGACTCG 
CTACAGAGGC AACTCTCCAT CGCACTGCCA CAGGAGTTTG CGGTGTTGAC CAAAGAGCTG 
AATGTATGTC GGGAGCAGCT GCTGGAGAGA GAAGAGGAGA TTGCTGAGCT CAAGGCAGAG 
AGGAACAACA CACGGCTCCT GCTGGAGCAT CTGGAGTGTC TGGTGTCCCG TCACGAGCGT 
TCTCTGAGGA TGACTGTGGT GAAGAGACAG GCTCAGTCTC CTGCTGGAGT GTCCAGTGAG 
GTTGAGGTGC TCAAGGCGCT CAAGTCCCTG TTTGAACACC ACAAAGCCCT GGATGAGAAG 
GTTCGTGAGA GATTGCGTGT GGCTTTAGAG AGAGTTGCCG TTCTAGAAGA AGAGCTTGAA 
TCATCTACGA ACGAGCGGCT CCCAAATGGC CCGTCCTCCA TCTTGGATGA TGGCGAGATT 
GACAGACAGA GAGAGGGAGA GATAGAGAGA CAGAGAGCAG AGCTTTCACA GTTAAAAGAA 
AGACTGGCGC TCATGTGCAG ACAGGTTGGA GAGATCGAGG AGCAGCTCAC CACCGCCAGG 
AGGGAATTAG CTAAATCTGA GGAGGCCAAT CAGAAGCTGC AGAGGGATGT GAAAGAGGCG 
CTGTGTCAAA GAGAGGACAT GGAAGAAAGA ATCACCACCT TAGAGCGCAG GTACCTCAGT 
GCCCAGAGGG AGGCAACCTC TCTCCATGAC ATCAAAGACA AACTGGAGAA CGAGCTCGCC 
AGCAAAGAGT CTTTATACAG ACAGAGTGAA GAGAAGAACC GGCAGCTTCA GGAGCGACTG 
GATGATGCCA AACAGAAGCT CCAGCAGACC CTCCAGAGGG CTGAGACGCT GCCCGAGATC 
GAGGCTCAGC TCGCCCAAAG GGTGGCGGCG CTCAATAAGG CGGAGGAGCG GCATGGCAAC 
TTTGAGGAGA GACTGAGACA GCTGGAGGCG CAACTCGAGG AGAAGAACCA GGAGCTGCAG 
AGGGCAAGGC AGAGGGAGCG CATGAATGAT GAACACAATA AACGTCTCTC CGACACGGTG 
GACAAGCTTC TGTCCGAGTC CAATGAGAGG CTTCAGCTGC ACCTGAAGGA GAGAATGGCT 
GCCCTCGAGG AGAAGAACGC TCTTTCTGAG GAACTCGCTA ACATGAAGAA GATCCAAGAC 
GATCTCTTAG CAAACAAGGA TCAACTAATT GCAGAGCTGG AGAGGCTTCA GCTAGAGCTG 
GACCAGCTGA GAGGAAGACC TGGATCCTCT TACTCACGAT CTCTGCCAGG AAGTGCTCTA 
GAGCTGAGGT ACTCTCAAGG AGGCGGGTCA CTTCCTGCCG GCTCCACCAC TCACCTAGAG 
CATTATGGAA ACACCGGCAC AGGGGGTGTG GTCAGACGGA CTCACCGTGG ACGCTGGAGT 
GGTGCCAGGG AGGATGCAAG CAAATATGGC GATTGGGAAA GCGGAGCGCT GCTGGGCTCT 
GGTTTCGAGG GCGGTGTGGA GGACAGGTGT TCTGATGATG AAGAGGACCG CGAGACGCTT 
TTCGGCTCGG AGCTGCTCTC ACCGAGCGGC CAGACAGATG TGCAGACCCT CGCTATCATG 
TTGCAGGAGC AGCTGGAGGC CATCAACAAA GAAATAAAGC TGATTCAGGA AGAGAAGGAG 
AGCACAGAGC TTCGTGCTGA AGAAATCGAG AGCAGAGTCA GCAGCGTGGC TCTGGACGGG 
CCTCCCATTC CACCGACCTC TCTGGGACGG GACAGCACAG GACGGGGCTT CATCCCCCCA 
TCCCTCACAT CCTCCACCCT GGCTTCACCC TCTCCGCCCA GCTCTGGGCA CTCCACACCC 
CGCCTGCCCC ATTCCCCCGC ACGCGAGACA GACAGACAGA ACAACAAAGA GGACGACAGG 
TCTCTGGCCT TACTGGACTC CACCCCTCCT CCCACCCCTC GTGCGCTACG ATTGGACAGG 
ATGACACACA CTCACCCTGG TGCATTGCTG GATGACAGTC GCGAGTTTCG CAGCCTCTCA 
GCTGATGGCA GCAGTTCAAA CAGCAGCCAG GACTCCCTCC ACAAGGCCAG TAAAAAGAAA 
AGCATCAAGT CTTCCATTGG TCGGCTCTTT GGAAAGAAGG ACAAGGTCCG AATGGGTCAG 
CCTGGGCGTG AATCAACATC TCTGGCCTCA ACCCCTTCAG ATGACTTGGG CACTGTGGAT 
CCCCTCGGAC TCACTAAGAT GGCCGGTCCG GTAGACAAGG ACCGGCGCAG CAAGAAGAAG 
CATGAGCTGC TGGAGGAAGC ATGTCGACAG GGTCTGCCCT TTGCATCCTG GGATGGACCC 
ACTGTTGTCT CCTGGCTGGA ACTGTGGGTG GGCATGCCGG CGTGGTATGT AGCAGCATGT 
CGTGCAAACG TGAAGAGTGG AGCCATCATG GCAAACCTGT CTGACACAGA GATCCAGAGA 
GAGATCGGCA TCAGTAATCC TCTGCACCGC CTCAAACTGA GACTGGCCAT TCAAGAGATG 
GTGTCCCTGA CCAGCCCCTC AGCCCCGGCT AGCACACGCT CCTCCACCAG TAACGTGTGG 
ATGACCCACG CTGAGATGGA GTCCCTGACT GCAGCAACCA AACCAGAGCT GAAAGAGATT 
AGCTGGGATC AGATCCTGGC CTATGGAGAC ATGAACCATG AGTGGGTGGG CAATGACTGG 
CTGCCCAGTC TGGGTTTGCC CCAGTACAGA AGCTACTTCA TGGAGTCTCT TGTGGATGCC 
CGCATGTTGG ACCATCTGAC CAAGAAAGAG CTGCGTGGCC AGCTGAAAAT GGTGGACAGT 
TTCCACAGGG TGAGCATGCA TTATGGCATC ATGTGCCTGA AGCGCCTCAA TTATGACCGC 
AAGGAGCTGG AGAGGAGGAG AGATGAGAGC CAGCACCAGA ATAAAGATGT GATGGTGTGG 
TCGAACGAGC GTGTGATGTG CTGGGTTCAG TCCATCGGCT TGAAGGAGTA CGCAGATAAC 
CTGACTGAGA GCGGTGTTCA TGGAGCGCTG TTGGCCCTGG ATGACACCTT CGATTACACT 
GACCTCGCAC TGCTCCTGCA GATTCCCAAT CAAAACACAC AGGCACGACA ACTTCTGGAG 
AAGGAATACA ACTCCCTCAT CTCAATGGGA ACAGAAAGGA GGCCAGACGA GGATGGCACT 
AAGACGTTCA CCCGCTCTCC TTCATGGAGG AAGATGTTTC GGGAGAAGGA CCTGCGGGGT 
GTGACGTCTG ACTCCTCCGA GACCCTGCCT GCTAACTTCC GTGCCTCAGC CATCTCTACC 
CCCTCAGTCA CGCTGAGGAA GGTCCAGAGC GATGCATCTC CAAGGGCAGA GTCTGGGTCA 
GTGAGGACCT ACTCCTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329710.1
CDS	884..4504
Translation	MMCEVMPTISEDGRGGAGGGPSSPAGGGGGMGGGAGGMGGGYGGREARGGDEGGSTGNLESLMVNMLTERERLLESLRETQESLGTAQLRLRELGHEKDSLQRQLSIALPQEFAVLTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERVAVLEEELESSTNERLPNGPSSILDDGEIDRQREGEIERQRAELSQLKERLALMCRQVGEIEEQLTTARRELAKSEEANQKLQRDVKEALCQREDMEERITTLERRYLSAQREATSLHDIKDKLENELASKESLYRQSEEKNRQLQERLDDAKQKLQQTLQRAETLPEIEAQLAQRVAALNKAEERHGNFEERLRQLEAQLEEKNQELQRARQRERMNDEHNKRLSDTVDKLLSESNERLQLHLKERMAALEEKNALSEELANMKKIQDDLLANKDQLIAELERLQLELDQLRGRPGSSYSRSLPGSALELRYSQGGGSLPAGSTTHLEHYGNTGTGGVVRRTHRGRWSGAREDASKYGDWESGALLGSGFEGGVEDRCSDDEEDRETLFGSELLSPSGQTDVQTLAIMLQEQLEAINKEIKLIQEEKESTELRAEEIESRVSSVALDGPPIPPTSLGRDSTGRGFIPPSLTSSTLASPSPPSSGHSTPRLPHSPARETDRQNNKEDDRSLALLDSTPPPTPRALRLDRMTHTHPGALLDDSREFRSLSADGSSSNSSQDSLHKASKKKSIKSSIGRLFGKKDKVRMGQPGRESTSLASTPSDDLGTVDPLGLTKMAGPVDKDRRSKKKHELLEEACRQGLPFASWDGPTVVSWLELWVGMPAWYVAACRANVKSGAIMANLSDTEIQREIGISNPLHRLKLRLAIQEMVSLTSPSAPASTRSSTSNVWMTHAEMESLTAATKPELKEISWDQILAYGDMNHEWVGNDWLPSLGLPQYRSYFMESLVDARMLDHLTKKELRGQLKMVDSFHRVSMHYGIMCLKRLNYDRKELERRRDESQHQNKDVMVWSNERVMCWVQSIGLKEYADNLTESGVHGALLALDDTFDYTDLALLLQIPNQNTQARQLLEKEYNSLISMGTERRPDEDGTKTFTRSPSWRKMFREKDLRGVTSDSSETLPANFRASAISTPSVTLRKVQSDASPRAESGSVRTYSC

Target ORF information:

RefSeq Version	XM_021474035.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3 (ppfia3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474035.1

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC CACCATCTCC GAGGACGGGC GGGGTGGTGC TGGAGGTGGC 
CCGTCCTCTC CTGCTGGAGG TGGTGGGGGT ATGGGGGGCG GTGCAGGGGG CATGGGTGGT 
GGCTATGGTG GCAGGGAGGC CCGCGGCGGA GATGAAGGAG GGAGCACTGG AAACCTGGAG 
TCTCTGATGG TGAACATGCT GACGGAGAGA GAGCGGCTGC TGGAGAGTCT CCGAGAGACC 
CAGGAGAGTC TCGGCACGGC ACAGCTACGT CTGCGAGAGC TGGGTCACGA GAAGGACTCG 
CTACAGAGGC AACTCTCCAT CGCACTGCCA CAGGAGTTTG CGGTGTTGAC CAAAGAGCTG 
AATGTATGTC GGGAGCAGCT GCTGGAGAGA GAAGAGGAGA TTGCTGAGCT CAAGGCAGAG 
AGGAACAACA CACGGCTCCT GCTGGAGCAT CTGGAGTGTC TGGTGTCCCG TCACGAGCGT 
TCTCTGAGGA TGACTGTGGT GAAGAGACAG GCTCAGTCTC CTGCTGGAGT GTCCAGTGAG 
GTTGAGGTGC TCAAGGCGCT CAAGTCCCTG TTTGAACACC ACAAAGCCCT GGATGAGAAG 
GTTCGTGAGA GATTGCGTGT GGCTTTAGAG AGAGTTGCCG TTCTAGAAGA AGAGCTTGAA 
TCATCTACGA ACGAGCGGCT CCCAAATGGC CCGTCCTCCA TCTTGGATGA TGGCGAGATT 
GACAGACAGA GAGAGGGAGA GATAGAGAGA CAGAGAGCAG AGCTTTCACA GTTAAAAGAA 
AGACTGGCGC TCATGTGCAG ACAGGTTGGA GAGATCGAGG AGCAGCTCAC CACCGCCAGG 
AGGGAATTAG CTAAATCTGA GGAGGCCAAT CAGAAGCTGC AGAGGGATGT GAAAGAGGCG 
CTGTGTCAAA GAGAGGACAT GGAAGAAAGA ATCACCACCT TAGAGCGCAG GTACCTCAGT 
GCCCAGAGGG AGGCAACCTC TCTCCATGAC ATCAAAGACA AACTGGAGAA CGAGCTCGCC 
AGCAAAGAGT CTTTATACAG ACAGAGTGAA GAGAAGAACC GGCAGCTTCA GGAGCGACTG 
GATGATGCCA AACAGAAGCT CCAGCAGACC CTCCAGAGGG CTGAGACGCT GCCCGAGATC 
GAGGCTCAGC TCGCCCAAAG GGTGGCGGCG CTCAATAAGG CGGAGGAGCG GCATGGCAAC 
TTTGAGGAGA GACTGAGACA GCTGGAGGCG CAACTCGAGG AGAAGAACCA GGAGCTGCAG 
AGGGCAAGGC AGAGGGAGCG CATGAATGAT GAACACAATA AACGTCTCTC CGACACGGTG 
GACAAGCTTC TGTCCGAGTC CAATGAGAGG CTTCAGCTGC ACCTGAAGGA GAGAATGGCT 
GCCCTCGAGG AGAAGAACGC TCTTTCTGAG GAACTCGCTA ACATGAAGAA GATCCAAGAC 
GATCTCTTAG CAAACAAGGA TCAACTAATT GCAGAGCTGG AGAGGCTTCA GCTAGAGCTG 
GACCAGCTGA GAGGAAGACC TGGATCCTCT TACTCACGAT CTCTGCCAGG AAGTGCTCTA 
GAGCTGAGGT ACTCTCAAGG AGGCGGGTCA CTTCCTGCCG GCTCCACCAC TCACCTAGAG 
CATTATGGAA ACACCGGCAC AGGGGGTGTG GTCAGACGGA CTCACCGTGG ACGCTGGAGT 
GGTGCCAGGG AGGATGCAAG CAAATATGGC GATTGGGAAA GCGGAGCGCT GCTGGGCTCT 
GGTTTCGAGG GCGGTGTGGA GGACAGGTGT TCTGATGATG AAGAGGACCG CGAGACGCTT 
TTCGGCTCGG AGCTGCTCTC ACCGAGCGGC CAGACAGATG TGCAGACCCT CGCTATCATG 
TTGCAGGAGC AGCTGGAGGC CATCAACAAA GAAATAAAGC TGATTCAGGA AGAGAAGGAG 
AGCACAGAGC TTCGTGCTGA AGAAATCGAG AGCAGAGTCA GCAGCGTGGC TCTGGACGGG 
CCTCCCATTC CACCGACCTC TCTGGGACGG GACAGCACAG GACGGGGCTT CATCCCCCCA 
TCCCTCACAT CCTCCACCCT GGCTTCACCC TCTCCGCCCA GCTCTGGGCA CTCCACACCC 
CGCCTGCCCC ATTCCCCCGC ACGCGAGACA GACAGACAGA ACAACAAAGA GGACGACAGG 
TCTCTGGCCT TACTGGACTC CACCCCTCCT CCCACCCCTC GTGCGCTACG ATTGGACAGG 
ATGACACACA CTCACCCTGG TGCATTGCTG GATGACAGTC GCGAGTTTCG CAGCCTCTCA 
GCTGATGGCA GCAGTTCAAA CAGCAGCCAG GACTCCCTCC ACAAGGCCAG TAAAAAGAAA 
AGCATCAAGT CTTCCATTGG TCGGCTCTTT GGAAAGAAGG ACAAGGTCCG AATGGGTCAG 
CCTGGGCGTG AATCAACATC TCTGGCCTCA ACCCCTTCAG ATGACTTGGG CACTGTGGAT 
CCCCTCGGAC TCACTAAGAT GGCCGGTCCG GTAGACAAGG ACCGGCGCAG CAAGAAGAAG 
CATGAGCTGC TGGAGGAAGC ATGTCGACAG GGTCTGCCCT TTGCATCCTG GGATGGACCC 
ACTGTTGTCT CCTGGCTGGA ACTGTGGGTG GGCATGCCGG CGTGGTATGT AGCAGCATGT 
CGTGCAAACG TGAAGAGTGG AGCCATCATG GCAAACCTGT CTGACACAGA GATCCAGAGA 
GAGATCGGCA TCAGTAATCC TCTGCACCGC CTCAAACTGA GACTGGCCAT TCAAGAGATG 
GTGTCCCTGA CCAGCCCCTC AGCCCCGGCT AGCACACGCT CCTCCACCAG TAACGTGTGG 
ATGACCCACG CTGAGATGGA GTCCCTGACT GCAGCAACCA AACCAGAGCT GAAAGAGATT 
AGCTGGGATC AGATCCTGGC CTATGGAGAC ATGAACCATG AGTGGGTGGG CAATGACTGG 
CTGCCCAGTC TGGGTTTGCC CCAGTACAGA AGCTACTTCA TGGAGTCTCT TGTGGATGCC 
CGCATGTTGG ACCATCTGAC CAAGAAAGAG CTGCGTGGCC AGCTGAAAAT GGTGGACAGT 
TTCCACAGGG TGAGCATGCA TTATGGCATC ATGTGCCTGA AGCGCCTCAA TTATGACCGC 
AAGGAGCTGG AGAGGAGGAG AGATGAGAGC CAGCACCAGA ATAAAGATGT GATGGTGTGG 
TCGAACGAGC GTGTGATGTG CTGGGTTCAG TCCATCGGCT TGAAGGAGTA CGCAGATAAC 
CTGACTGAGA GCGGTGTTCA TGGAGCGCTG TTGGCCCTGG ATGACACCTT CGATTACACT 
GACCTCGCAC TGCTCCTGCA GATTCCCAAT CAAAACACAC AGGCACGACA ACTTCTGGAG 
AAGGAATACA ACTCCCTCAT CTCAATGGGA ACAGAAAGGA GGCCAGACGA GGATGGCACT 
AAGACGTTCA CCCGCTCTCC TTCATGGAGG AAGATGTTTC GGGAGAAGGA CCTGCGGGGT 
GTGACGTCTG ACTCCTCCGA GACCCTGCCT GCTAACTTCC GTGCCTCAGC CATCTCTACC 
CCCTCAGTCA CGCTGAGGAA GGTCCAGAGC GATGCATCTC CAAGGGCAGA GTCTGGGTCA 
GTGAGGACCT ACTCCTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ppfia3 ( XM_003198113.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003198161.2 Danio rerio protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3 (ppfia3), mRNA.

Price & Availability↑

CloneID	ODa08389
Clone ID Related Accession (Same CDS sequence)	XM_003198113.4 , XM_003198113.5
Accession Version	XM_003198113.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	liprin-alpha-3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003198113.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC CACCATCTCC GAGGACGGGC GGGGTGGTGC TGGAGGTGGC 
CCGTCCTCTC CTGCTGGAGG TGGTGGGGGT ATGGGGGGCG GTGCAGGGGG CATGGGTGGT 
GGCTATGGTG GCAGGGAGGC CCGCGGCGGA GATGAAGGAG GGAGCACTGG AAACCTGGAG 
TCTCTGATGG TGAACATGCT GACGGAGAGA GAGCGGCTGC TGGAGAGTCT CCGAGAGACC 
CAGGAGAGTC TCGGCACGGC ACAGCTACGT CTGCGAGAGC TGGGTCACGA GAAGGACTCG 
CTACAGAGGC AACTCTCCAT CGCACTGCCA CAGGAGTTTG CGGTGTTGAC CAAAGAGCTG 
AATGTATGTC GGGAGCAGCT GCTGGAGAGA GAAGAGGAGA TTGCTGAGCT CAAGGCAGAG 
AGGAACAACA CACGGCTCCT GCTGGAGCAT CTGGAGTGTC TGGTGTCCCG TCACGAGCGT 
TCTCTGAGGA TGACTGTGGT GAAGAGACAG GCTCAGTCTC CTGCTGGAGT GTCCAGTGAG 
GTTGAGGTGC TCAAGGCGCT CAAGTCCCTG TTTGAACACC ACAAAGCCCT GGATGAGAAG 
GTTCGTGAGA GATTGCGTGT GGCTTTAGAG AGAGTTGCCG TTCTAGAAGA AGAGCTTGAA 
TCATCTACGA ACGAGGTACT GTCTTTGCGG GATCAGATTA AAAGGAGAGA GCGGGGTTTG 
GACAATGGAA AGGAGCGGCT CCCAAATGGC CCGTCCTCCA TCTTGGATGA TGGCGAGATT 
GACAGACAGA GAGAGGGAGA GATAGAGAGA CAGAGAGCAG AGCTTTCACA GTTAAAAGAA 
AGACTGGCGC TCATGTGCAG ACAGGTTGGA GAGATCGAGG AGCAGCTCAC CACCGCCAGG 
AGGGAATTAG CTAAATCTGA GGAGGCCAAT CAGAAGCTGC AGAGGGATGT GAAAGAGGCG 
CTGTGTCAAA GAGAGGACAT GGAAGAAAGA ATCACCACCT TAGAGCGCAG GTACCTCAGT 
GCCCAGAGGG AGGCAACCTC TCTCCATGAC ATCAAAGACA AACTGGAGAA CGAGCTCGCC 
AGCAAAGAGT CTTTATACAG ACAGAGTGAA GAGAAGAACC GGCAGCTTCA GGAGCGACTG 
GATGATGCCA AACAGAAGCT CCAGCAGACC CTCCAGAGGG CTGAGACGCT GCCCGAGATC 
GAGGCTCAGC TCGCCCAAAG GGTGGCGGCG CTCAATAAGG CGGAGGAGCG GCATGGCAAC 
TTTGAGGAGA GACTGAGACA GCTGGAGGCG CAACTCGAGG AGAAGAACCA GGAGCTGCAG 
AGGGCAAGGC AGAGGGAGCG CATGAATGAT GAACACAATA AACGTCTCTC CGACACGGTG 
GACAAGCTTC TGTCCGAGTC CAATGAGAGG CTTCAGCTGC ACCTGAAGGA GAGAATGGCT 
GCCCTCGAGG AGAAGAACGC TCTTTCTGAG GAACTCGCTA ACATGAAGAA GATCCAAGAC 
GATCTCTTAG CAAACAAGGA TCAACTAATT GCAGAGCTGG AGAGGCTTCA GCTAGAGCTG 
GACCAGCTGA GAGGAAGACC TGGATCCTCT TACTCACGAT CTCTGCCAGG AAGTGCTCTA 
GAGCTGAGGT ACTCTCAAGG AGGCGGGTCA CTTCCTGCCG GCTCCACCAC TCACCTAGAG 
CATTATGGAA ACACCGGCAC AGGGGGTGTG GTCAGACGGA CTCACCGTGG ACGCTGGAGT 
GGTGCCAGGG AGGATGCAAG CAAATATGGC GATTGGGAAA GCGGAGCGCT GCTGGGCTCT 
GGTTTCGAGG GCGGTGTGGA GGACAGGTGT TCTGATGATG AAGAGGACCG CGAGACGCTT 
TTCGGCTCGG AGCTGCTCTC ACCGAGCGGC CAGACAGATG TGCAGACCCT CGCTATCATG 
TTGCAGGAGC AGCTGGAGGC CATCAACAAA GAAATAAAGC TGATTCAGGA AGAGAAGGAG 
AGCACAGAGC TTCGTGCTGA AGAAATCGAG AGCAGAGTCA GCAGCGTGGC TCTGGACGGG 
CCTCCCATTC CACCGACCTC TCTGGGACGG GACAGCACAG GACGGGGCTT CATCCCCCCA 
TCCCTCACAT CCTCCACCCT GGCTTCACCC TCTCCGCCCA GCTCTGGGCA CTCCACACCC 
CGCCTGCCCC ATTCCCCCGC ACGCGAGACA GACAGACAGA ACAACAAAGA GGACGACAGG 
TCTCTGGCCT TACTGGACTC CACCCCTCCT CCCACCCCTC GTGCGCTACG ATTGGACAGG 
ATGACACACA CTCACCCTGG TGCATTGCTG GATGACAGTC GCGAGTTTCG CAGCCTCTCA 
GCTGATGGCA GCAGTTCAAA CAGCAGCCAG GACTCCCTCC ACAAGGCCAG TAAAAAGAAA 
AGCATCAAGT CTTCCATTGG TCGGCTCTTT GGAAAGAAGG ACAAGGTCCG AATGGGTCAG 
CCTGGGCGTG AATCAACATC TCTGGCCTCA ACCCCTTCAG ATGACTTGGG CACTGTGGAT 
CCCCTCGGAC TCACTAAGAT GGCCGGTCCG GTAGACAAGG ACCGGCGCAG CAAGAAGAAG 
CATGAGCTGC TGGAGGAAGC ATGTCGACAG GGTCTGCCCT TTGCATCCTG GGATGGACCC 
ACTGTTGTCT CCTGGCTGGA ACTGTGGGTG GGCATGCCGG CGTGGTATGT AGCAGCATGT 
CGTGCAAACG TGAAGAGTGG AGCCATCATG GCAAACCTGT CTGACACAGA GATCCAGAGA 
GAGATCGGCA TCAGTAATCC TCTGCACCGC CTCAAACTGA GACTGGCCAT TCAAGAGATG 
GTGTCCCTGA CCAGCCCCTC AGCCCCGGCT AGCACACGCT CCTCCACCAG TAACGTGTGG 
ATGACCCACG CTGAGATGGA GTCCCTGACT GCAGCAACCA AACCAGAGCT GAAAGAGATT 
AGCTGGGATC AGATCCTGGC CTATGGAGAC ATGAACCATG AGTGGGTGGG CAATGACTGG 
CTGCCCAGTC TGGGTTTGCC CCAGTACAGA AGCTACTTCA TGGAGTCTCT TGTGGATGCC 
CGCATGTTGG ACCATCTGAC CAAGAAAGAG CTGCGTGGCC AGCTGAAAAT GGTGGACAGT 
TTCCACAGGG TGAGCATGCA TTATGGCATC ATGTGCCTGA AGCGCCTCAA TTATGACCGC 
AAGGAGCTGG AGAGGAGGAG AGATGAGAGC CAGCACCAGA ATAAAGATGT GATGGTGTGG 
TCGAACGAGC GTGTGATGTG CTGGGTTCAG TCCATCGGCT TGAAGGAGTA CGCAGATAAC 
CTGACTGAGA GCGGTGTTCA TGGAGCGCTG TTGGCCCTGG ATGACACCTT CGATTACACT 
GACCTCGCAC TGCTCCTGCA GATTCCCAAT CAAAACACAC AGGCACGACA ACTTCTGGAG 
AAGGAATACA ACTCCCTCAT CTCAATGGGA ACAGAAAGGA GGCCAGACGA GGATGGCACT 
AAGACGTTCA CCCGCTCTCC TTCATGGAGG AAGATGTTTC GGGAGAAGGA CCTGCGGGGT 
GTGACGTCTG ACTCCTCCGA GACCCTGCCT GCTAACTTCC GTGCCTCAGC CATCTCTACC 
CCCTCAGTCA CGCTGAGGAA GGTCCAGAGC GATGCATCTC CAAGGGCAGA GTCTGGGTCA 
GTGAGGACCT ACTCCTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003198161.2
CDS	892..4572
Translation	MMCEVMPTISEDGRGGAGGGPSSPAGGGGGMGGGAGGMGGGYGGREARGGDEGGSTGNLESLMVNMLTERERLLESLRETQESLGTAQLRLRELGHEKDSLQRQLSIALPQEFAVLTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERVAVLEEELESSTNEVLSLRDQIKRRERGLDNGKERLPNGPSSILDDGEIDRQREGEIERQRAELSQLKERLALMCRQVGEIEEQLTTARRELAKSEEANQKLQRDVKEALCQREDMEERITTLERRYLSAQREATSLHDIKDKLENELASKESLYRQSEEKNRQLQERLDDAKQKLQQTLQRAETLPEIEAQLAQRVAALNKAEERHGNFEERLRQLEAQLEEKNQELQRARQRERMNDEHNKRLSDTVDKLLSESNERLQLHLKERMAALEEKNALSEELANMKKIQDDLLANKDQLIAELERLQLELDQLRGRPGSSYSRSLPGSALELRYSQGGGSLPAGSTTHLEHYGNTGTGGVVRRTHRGRWSGAREDASKYGDWESGALLGSGFEGGVEDRCSDDEEDRETLFGSELLSPSGQTDVQTLAIMLQEQLEAINKEIKLIQEEKESTELRAEEIESRVSSVALDGPPIPPTSLGRDSTGRGFIPPSLTSSTLASPSPPSSGHSTPRLPHSPARETDRQNNKEDDRSLALLDSTPPPTPRALRLDRMTHTHPGALLDDSREFRSLSADGSSSNSSQDSLHKASKKKSIKSSIGRLFGKKDKVRMGQPGRESTSLASTPSDDLGTVDPLGLTKMAGPVDKDRRSKKKHELLEEACRQGLPFASWDGPTVVSWLELWVGMPAWYVAACRANVKSGAIMANLSDTEIQREIGISNPLHRLKLRLAIQEMVSLTSPSAPASTRSSTSNVWMTHAEMESLTAATKPELKEISWDQILAYGDMNHEWVGNDWLPSLGLPQYRSYFMESLVDARMLDHLTKKELRGQLKMVDSFHRVSMHYGIMCLKRLNYDRKELERRRDESQHQNKDVMVWSNERVMCWVQSIGLKEYADNLTESGVHGALLALDDTFDYTDLALLLQIPNQNTQARQLLEKEYNSLISMGTERRPDEDGTKTFTRSPSWRKMFREKDLRGVTSDSSETLPANFRASAISTPSVTLRKVQSDASPRAESGSVRTYSC

Target ORF information:

RefSeq Version	XM_003198113.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3 (ppfia3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003198113.4

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC CACCATCTCC GAGGACGGGC GGGGTGGTGC TGGAGGTGGC 
CCGTCCTCTC CTGCTGGAGG TGGTGGGGGT ATGGGGGGCG GTGCAGGGGG CATGGGTGGT 
GGCTATGGTG GCAGGGAGGC CCGCGGCGGA GATGAAGGAG GGAGCACTGG AAACCTGGAG 
TCTCTGATGG TGAACATGCT GACGGAGAGA GAGCGGCTGC TGGAGAGTCT CCGAGAGACC 
CAGGAGAGTC TCGGCACGGC ACAGCTACGT CTGCGAGAGC TGGGTCACGA GAAGGACTCG 
CTACAGAGGC AACTCTCCAT CGCACTGCCA CAGGAGTTTG CGGTGTTGAC CAAAGAGCTG 
AATGTATGTC GGGAGCAGCT GCTGGAGAGA GAAGAGGAGA TTGCTGAGCT CAAGGCAGAG 
AGGAACAACA CACGGCTCCT GCTGGAGCAT CTGGAGTGTC TGGTGTCCCG TCACGAGCGT 
TCTCTGAGGA TGACTGTGGT GAAGAGACAG GCTCAGTCTC CTGCTGGAGT GTCCAGTGAG 
GTTGAGGTGC TCAAGGCGCT CAAGTCCCTG TTTGAACACC ACAAAGCCCT GGATGAGAAG 
GTTCGTGAGA GATTGCGTGT GGCTTTAGAG AGAGTTGCCG TTCTAGAAGA AGAGCTTGAA 
TCATCTACGA ACGAGGTACT GTCTTTGCGG GATCAGATTA AAAGGAGAGA GCGGGGTTTG 
GACAATGGAA AGGAGCGGCT CCCAAATGGC CCGTCCTCCA TCTTGGATGA TGGCGAGATT 
GACAGACAGA GAGAGGGAGA GATAGAGAGA CAGAGAGCAG AGCTTTCACA GTTAAAAGAA 
AGACTGGCGC TCATGTGCAG ACAGGTTGGA GAGATCGAGG AGCAGCTCAC CACCGCCAGG 
AGGGAATTAG CTAAATCTGA GGAGGCCAAT CAGAAGCTGC AGAGGGATGT GAAAGAGGCG 
CTGTGTCAAA GAGAGGACAT GGAAGAAAGA ATCACCACCT TAGAGCGCAG GTACCTCAGT 
GCCCAGAGGG AGGCAACCTC TCTCCATGAC ATCAAAGACA AACTGGAGAA CGAGCTCGCC 
AGCAAAGAGT CTTTATACAG ACAGAGTGAA GAGAAGAACC GGCAGCTTCA GGAGCGACTG 
GATGATGCCA AACAGAAGCT CCAGCAGACC CTCCAGAGGG CTGAGACGCT GCCCGAGATC 
GAGGCTCAGC TCGCCCAAAG GGTGGCGGCG CTCAATAAGG CGGAGGAGCG GCATGGCAAC 
TTTGAGGAGA GACTGAGACA GCTGGAGGCG CAACTCGAGG AGAAGAACCA GGAGCTGCAG 
AGGGCAAGGC AGAGGGAGCG CATGAATGAT GAACACAATA AACGTCTCTC CGACACGGTG 
GACAAGCTTC TGTCCGAGTC CAATGAGAGG CTTCAGCTGC ACCTGAAGGA GAGAATGGCT 
GCCCTCGAGG AGAAGAACGC TCTTTCTGAG GAACTCGCTA ACATGAAGAA GATCCAAGAC 
GATCTCTTAG CAAACAAGGA TCAACTAATT GCAGAGCTGG AGAGGCTTCA GCTAGAGCTG 
GACCAGCTGA GAGGAAGACC TGGATCCTCT TACTCACGAT CTCTGCCAGG AAGTGCTCTA 
GAGCTGAGGT ACTCTCAAGG AGGCGGGTCA CTTCCTGCCG GCTCCACCAC TCACCTAGAG 
CATTATGGAA ACACCGGCAC AGGGGGTGTG GTCAGACGGA CTCACCGTGG ACGCTGGAGT 
GGTGCCAGGG AGGATGCAAG CAAATATGGC GATTGGGAAA GCGGAGCGCT GCTGGGCTCT 
GGTTTCGAGG GCGGTGTGGA GGACAGGTGT TCTGATGATG AAGAGGACCG CGAGACGCTT 
TTCGGCTCGG AGCTGCTCTC ACCGAGCGGC CAGACAGATG TGCAGACCCT CGCTATCATG 
TTGCAGGAGC AGCTGGAGGC CATCAACAAA GAAATAAAGC TGATTCAGGA AGAGAAGGAG 
AGCACAGAGC TTCGTGCTGA AGAAATCGAG AGCAGAGTCA GCAGCGTGGC TCTGGACGGG 
CCTCCCATTC CACCGACCTC TCTGGGACGG GACAGCACAG GACGGGGCTT CATCCCCCCA 
TCCCTCACAT CCTCCACCCT GGCTTCACCC TCTCCGCCCA GCTCTGGGCA CTCCACACCC 
CGCCTGCCCC ATTCCCCCGC ACGCGAGACA GACAGACAGA ACAACAAAGA GGACGACAGG 
TCTCTGGCCT TACTGGACTC CACCCCTCCT CCCACCCCTC GTGCGCTACG ATTGGACAGG 
ATGACACACA CTCACCCTGG TGCATTGCTG GATGACAGTC GCGAGTTTCG CAGCCTCTCA 
GCTGATGGCA GCAGTTCAAA CAGCAGCCAG GACTCCCTCC ACAAGGCCAG TAAAAAGAAA 
AGCATCAAGT CTTCCATTGG TCGGCTCTTT GGAAAGAAGG ACAAGGTCCG AATGGGTCAG 
CCTGGGCGTG AATCAACATC TCTGGCCTCA ACCCCTTCAG ATGACTTGGG CACTGTGGAT 
CCCCTCGGAC TCACTAAGAT GGCCGGTCCG GTAGACAAGG ACCGGCGCAG CAAGAAGAAG 
CATGAGCTGC TGGAGGAAGC ATGTCGACAG GGTCTGCCCT TTGCATCCTG GGATGGACCC 
ACTGTTGTCT CCTGGCTGGA ACTGTGGGTG GGCATGCCGG CGTGGTATGT AGCAGCATGT 
CGTGCAAACG TGAAGAGTGG AGCCATCATG GCAAACCTGT CTGACACAGA GATCCAGAGA 
GAGATCGGCA TCAGTAATCC TCTGCACCGC CTCAAACTGA GACTGGCCAT TCAAGAGATG 
GTGTCCCTGA CCAGCCCCTC AGCCCCGGCT AGCACACGCT CCTCCACCAG TAACGTGTGG 
ATGACCCACG CTGAGATGGA GTCCCTGACT GCAGCAACCA AACCAGAGCT GAAAGAGATT 
AGCTGGGATC AGATCCTGGC CTATGGAGAC ATGAACCATG AGTGGGTGGG CAATGACTGG 
CTGCCCAGTC TGGGTTTGCC CCAGTACAGA AGCTACTTCA TGGAGTCTCT TGTGGATGCC 
CGCATGTTGG ACCATCTGAC CAAGAAAGAG CTGCGTGGCC AGCTGAAAAT GGTGGACAGT 
TTCCACAGGG TGAGCATGCA TTATGGCATC ATGTGCCTGA AGCGCCTCAA TTATGACCGC 
AAGGAGCTGG AGAGGAGGAG AGATGAGAGC CAGCACCAGA ATAAAGATGT GATGGTGTGG 
TCGAACGAGC GTGTGATGTG CTGGGTTCAG TCCATCGGCT TGAAGGAGTA CGCAGATAAC 
CTGACTGAGA GCGGTGTTCA TGGAGCGCTG TTGGCCCTGG ATGACACCTT CGATTACACT 
GACCTCGCAC TGCTCCTGCA GATTCCCAAT CAAAACACAC AGGCACGACA ACTTCTGGAG 
AAGGAATACA ACTCCCTCAT CTCAATGGGA ACAGAAAGGA GGCCAGACGA GGATGGCACT 
AAGACGTTCA CCCGCTCTCC TTCATGGAGG AAGATGTTTC GGGAGAAGGA CCTGCGGGGT 
GTGACGTCTG ACTCCTCCGA GACCCTGCCT GCTAACTTCC GTGCCTCAGC CATCTCTACC 
CCCTCAGTCA CGCTGAGGAA GGTCCAGAGC GATGCATCTC CAAGGGCAGA GTCTGGGTCA 
GTGAGGACCT ACTCCTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ppfia3 ( XM_003198113.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003198161.2 Danio rerio protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3 (ppfia3), transcript variant X1, mRNA.

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CloneID	ODa08389
Clone ID Related Accession (Same CDS sequence)	XM_003198113.4 , XM_003198113.5
Accession Version	XM_003198113.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	liprin-alpha-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003198113.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTGCG AGGTGATGCC CACCATCTCC GAGGACGGGC GGGGTGGTGC TGGAGGTGGC 
CCGTCCTCTC CTGCTGGAGG TGGTGGGGGT ATGGGGGGCG GTGCAGGGGG CATGGGTGGT 
GGCTATGGTG GCAGGGAGGC CCGCGGCGGA GATGAAGGAG GGAGCACTGG AAACCTGGAG 
TCTCTGATGG TGAACATGCT GACGGAGAGA GAGCGGCTGC TGGAGAGTCT CCGAGAGACC 
CAGGAGAGTC TCGGCACGGC ACAGCTACGT CTGCGAGAGC TGGGTCACGA GAAGGACTCG 
CTACAGAGGC AACTCTCCAT CGCACTGCCA CAGGAGTTTG CGGTGTTGAC CAAAGAGCTG 
AATGTATGTC GGGAGCAGCT GCTGGAGAGA GAAGAGGAGA TTGCTGAGCT CAAGGCAGAG 
AGGAACAACA CACGGCTCCT GCTGGAGCAT CTGGAGTGTC TGGTGTCCCG TCACGAGCGT 
TCTCTGAGGA TGACTGTGGT GAAGAGACAG GCTCAGTCTC CTGCTGGAGT GTCCAGTGAG 
GTTGAGGTGC TCAAGGCGCT CAAGTCCCTG TTTGAACACC ACAAAGCCCT GGATGAGAAG 
GTTCGTGAGA GATTGCGTGT GGCTTTAGAG AGAGTTGCCG TTCTAGAAGA AGAGCTTGAA 
TCATCTACGA ACGAGGTACT GTCTTTGCGG GATCAGATTA AAAGGAGAGA GCGGGGTTTG 
GACAATGGAA AGGAGCGGCT CCCAAATGGC CCGTCCTCCA TCTTGGATGA TGGCGAGATT 
GACAGACAGA GAGAGGGAGA GATAGAGAGA CAGAGAGCAG AGCTTTCACA GTTAAAAGAA 
AGACTGGCGC TCATGTGCAG ACAGGTTGGA GAGATCGAGG AGCAGCTCAC CACCGCCAGG 
AGGGAATTAG CTAAATCTGA GGAGGCCAAT CAGAAGCTGC AGAGGGATGT GAAAGAGGCG 
CTGTGTCAAA GAGAGGACAT GGAAGAAAGA ATCACCACCT TAGAGCGCAG GTACCTCAGT 
GCCCAGAGGG AGGCAACCTC TCTCCATGAC ATCAAAGACA AACTGGAGAA CGAGCTCGCC 
AGCAAAGAGT CTTTATACAG ACAGAGTGAA GAGAAGAACC GGCAGCTTCA GGAGCGACTG 
GATGATGCCA AACAGAAGCT CCAGCAGACC CTCCAGAGGG CTGAGACGCT GCCCGAGATC 
GAGGCTCAGC TCGCCCAAAG GGTGGCGGCG CTCAATAAGG CGGAGGAGCG GCATGGCAAC 
TTTGAGGAGA GACTGAGACA GCTGGAGGCG CAACTCGAGG AGAAGAACCA GGAGCTGCAG 
AGGGCAAGGC AGAGGGAGCG CATGAATGAT GAACACAATA AACGTCTCTC CGACACGGTG 
GACAAGCTTC TGTCCGAGTC CAATGAGAGG CTTCAGCTGC ACCTGAAGGA GAGAATGGCT 
GCCCTCGAGG AGAAGAACGC TCTTTCTGAG GAACTCGCTA ACATGAAGAA GATCCAAGAC 
GATCTCTTAG CAAACAAGGA TCAACTAATT GCAGAGCTGG AGAGGCTTCA GCTAGAGCTG 
GACCAGCTGA GAGGAAGACC TGGATCCTCT TACTCACGAT CTCTGCCAGG AAGTGCTCTA 
GAGCTGAGGT ACTCTCAAGG AGGCGGGTCA CTTCCTGCCG GCTCCACCAC TCACCTAGAG 
CATTATGGAA ACACCGGCAC AGGGGGTGTG GTCAGACGGA CTCACCGTGG ACGCTGGAGT 
GGTGCCAGGG AGGATGCAAG CAAATATGGC GATTGGGAAA GCGGAGCGCT GCTGGGCTCT 
GGTTTCGAGG GCGGTGTGGA GGACAGGTGT TCTGATGATG AAGAGGACCG CGAGACGCTT 
TTCGGCTCGG AGCTGCTCTC ACCGAGCGGC CAGACAGATG TGCAGACCCT CGCTATCATG 
TTGCAGGAGC AGCTGGAGGC CATCAACAAA GAAATAAAGC TGATTCAGGA AGAGAAGGAG 
AGCACAGAGC TTCGTGCTGA AGAAATCGAG AGCAGAGTCA GCAGCGTGGC TCTGGACGGG 
CCTCCCATTC CACCGACCTC TCTGGGACGG GACAGCACAG GACGGGGCTT CATCCCCCCA 
TCCCTCACAT CCTCCACCCT GGCTTCACCC TCTCCGCCCA GCTCTGGGCA CTCCACACCC 
CGCCTGCCCC ATTCCCCCGC ACGCGAGACA GACAGACAGA ACAACAAAGA GGACGACAGG 
TCTCTGGCCT TACTGGACTC CACCCCTCCT CCCACCCCTC GTGCGCTACG ATTGGACAGG 
ATGACACACA CTCACCCTGG TGCATTGCTG GATGACAGTC GCGAGTTTCG CAGCCTCTCA 
GCTGATGGCA GCAGTTCAAA CAGCAGCCAG GACTCCCTCC ACAAGGCCAG TAAAAAGAAA 
AGCATCAAGT CTTCCATTGG TCGGCTCTTT GGAAAGAAGG ACAAGGTCCG AATGGGTCAG 
CCTGGGCGTG AATCAACATC TCTGGCCTCA ACCCCTTCAG ATGACTTGGG CACTGTGGAT 
CCCCTCGGAC TCACTAAGAT GGCCGGTCCG GTAGACAAGG ACCGGCGCAG CAAGAAGAAG 
CATGAGCTGC TGGAGGAAGC ATGTCGACAG GGTCTGCCCT TTGCATCCTG GGATGGACCC 
ACTGTTGTCT CCTGGCTGGA ACTGTGGGTG GGCATGCCGG CGTGGTATGT AGCAGCATGT 
CGTGCAAACG TGAAGAGTGG AGCCATCATG GCAAACCTGT CTGACACAGA GATCCAGAGA 
GAGATCGGCA TCAGTAATCC TCTGCACCGC CTCAAACTGA GACTGGCCAT TCAAGAGATG 
GTGTCCCTGA CCAGCCCCTC AGCCCCGGCT AGCACACGCT CCTCCACCAG TAACGTGTGG 
ATGACCCACG CTGAGATGGA GTCCCTGACT GCAGCAACCA AACCAGAGCT GAAAGAGATT 
AGCTGGGATC AGATCCTGGC CTATGGAGAC ATGAACCATG AGTGGGTGGG CAATGACTGG 
CTGCCCAGTC TGGGTTTGCC CCAGTACAGA AGCTACTTCA TGGAGTCTCT TGTGGATGCC 
CGCATGTTGG ACCATCTGAC CAAGAAAGAG CTGCGTGGCC AGCTGAAAAT GGTGGACAGT 
TTCCACAGGG TGAGCATGCA TTATGGCATC ATGTGCCTGA AGCGCCTCAA TTATGACCGC 
AAGGAGCTGG AGAGGAGGAG AGATGAGAGC CAGCACCAGA ATAAAGATGT GATGGTGTGG 
TCGAACGAGC GTGTGATGTG CTGGGTTCAG TCCATCGGCT TGAAGGAGTA CGCAGATAAC 
CTGACTGAGA GCGGTGTTCA TGGAGCGCTG TTGGCCCTGG ATGACACCTT CGATTACACT 
GACCTCGCAC TGCTCCTGCA GATTCCCAAT CAAAACACAC AGGCACGACA ACTTCTGGAG 
AAGGAATACA ACTCCCTCAT CTCAATGGGA ACAGAAAGGA GGCCAGACGA GGATGGCACT 
AAGACGTTCA CCCGCTCTCC TTCATGGAGG AAGATGTTTC GGGAGAAGGA CCTGCGGGGT 
GTGACGTCTG ACTCCTCCGA GACCCTGCCT GCTAACTTCC GTGCCTCAGC CATCTCTACC 
CCCTCAGTCA CGCTGAGGAA GGTCCAGAGC GATGCATCTC CAAGGGCAGA GTCTGGGTCA 
GTGAGGACCT ACTCCTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003198161.2
CDS	883..4563
Translation	MMCEVMPTISEDGRGGAGGGPSSPAGGGGGMGGGAGGMGGGYGGREARGGDEGGSTGNLESLMVNMLTERERLLESLRETQESLGTAQLRLRELGHEKDSLQRQLSIALPQEFAVLTKELNVCREQLLEREEEIAELKAERNNTRLLLEHLECLVSRHERSLRMTVVKRQAQSPAGVSSEVEVLKALKSLFEHHKALDEKVRERLRVALERVAVLEEELESSTNEVLSLRDQIKRRERGLDNGKERLPNGPSSILDDGEIDRQREGEIERQRAELSQLKERLALMCRQVGEIEEQLTTARRELAKSEEANQKLQRDVKEALCQREDMEERITTLERRYLSAQREATSLHDIKDKLENELASKESLYRQSEEKNRQLQERLDDAKQKLQQTLQRAETLPEIEAQLAQRVAALNKAEERHGNFEERLRQLEAQLEEKNQELQRARQRERMNDEHNKRLSDTVDKLLSESNERLQLHLKERMAALEEKNALSEELANMKKIQDDLLANKDQLIAELERLQLELDQLRGRPGSSYSRSLPGSALELRYSQGGGSLPAGSTTHLEHYGNTGTGGVVRRTHRGRWSGAREDASKYGDWESGALLGSGFEGGVEDRCSDDEEDRETLFGSELLSPSGQTDVQTLAIMLQEQLEAINKEIKLIQEEKESTELRAEEIESRVSSVALDGPPIPPTSLGRDSTGRGFIPPSLTSSTLASPSPPSSGHSTPRLPHSPARETDRQNNKEDDRSLALLDSTPPPTPRALRLDRMTHTHPGALLDDSREFRSLSADGSSSNSSQDSLHKASKKKSIKSSIGRLFGKKDKVRMGQPGRESTSLASTPSDDLGTVDPLGLTKMAGPVDKDRRSKKKHELLEEACRQGLPFASWDGPTVVSWLELWVGMPAWYVAACRANVKSGAIMANLSDTEIQREIGISNPLHRLKLRLAIQEMVSLTSPSAPASTRSSTSNVWMTHAEMESLTAATKPELKEISWDQILAYGDMNHEWVGNDWLPSLGLPQYRSYFMESLVDARMLDHLTKKELRGQLKMVDSFHRVSMHYGIMCLKRLNYDRKELERRRDESQHQNKDVMVWSNERVMCWVQSIGLKEYADNLTESGVHGALLALDDTFDYTDLALLLQIPNQNTQARQLLEKEYNSLISMGTERRPDEDGTKTFTRSPSWRKMFREKDLRGVTSDSSETLPANFRASAISTPSVTLRKVQSDASPRAESGSVRTYSC

Target ORF information:

RefSeq Version	XM_003198113.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3 (ppfia3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003198113.5

ORF Insert Sequence:

ATGATGTGCG AGGTGATGCC CACCATCTCC GAGGACGGGC GGGGTGGTGC TGGAGGTGGC 
CCGTCCTCTC CTGCTGGAGG TGGTGGGGGT ATGGGGGGCG GTGCAGGGGG CATGGGTGGT 
GGCTATGGTG GCAGGGAGGC CCGCGGCGGA GATGAAGGAG GGAGCACTGG AAACCTGGAG 
TCTCTGATGG TGAACATGCT GACGGAGAGA GAGCGGCTGC TGGAGAGTCT CCGAGAGACC 
CAGGAGAGTC TCGGCACGGC ACAGCTACGT CTGCGAGAGC TGGGTCACGA GAAGGACTCG 
CTACAGAGGC AACTCTCCAT CGCACTGCCA CAGGAGTTTG CGGTGTTGAC CAAAGAGCTG 
AATGTATGTC GGGAGCAGCT GCTGGAGAGA GAAGAGGAGA TTGCTGAGCT CAAGGCAGAG 
AGGAACAACA CACGGCTCCT GCTGGAGCAT CTGGAGTGTC TGGTGTCCCG TCACGAGCGT 
TCTCTGAGGA TGACTGTGGT GAAGAGACAG GCTCAGTCTC CTGCTGGAGT GTCCAGTGAG 
GTTGAGGTGC TCAAGGCGCT CAAGTCCCTG TTTGAACACC ACAAAGCCCT GGATGAGAAG 
GTTCGTGAGA GATTGCGTGT GGCTTTAGAG AGAGTTGCCG TTCTAGAAGA AGAGCTTGAA 
TCATCTACGA ACGAGGTACT GTCTTTGCGG GATCAGATTA AAAGGAGAGA GCGGGGTTTG 
GACAATGGAA AGGAGCGGCT CCCAAATGGC CCGTCCTCCA TCTTGGATGA TGGCGAGATT 
GACAGACAGA GAGAGGGAGA GATAGAGAGA CAGAGAGCAG AGCTTTCACA GTTAAAAGAA 
AGACTGGCGC TCATGTGCAG ACAGGTTGGA GAGATCGAGG AGCAGCTCAC CACCGCCAGG 
AGGGAATTAG CTAAATCTGA GGAGGCCAAT CAGAAGCTGC AGAGGGATGT GAAAGAGGCG 
CTGTGTCAAA GAGAGGACAT GGAAGAAAGA ATCACCACCT TAGAGCGCAG GTACCTCAGT 
GCCCAGAGGG AGGCAACCTC TCTCCATGAC ATCAAAGACA AACTGGAGAA CGAGCTCGCC 
AGCAAAGAGT CTTTATACAG ACAGAGTGAA GAGAAGAACC GGCAGCTTCA GGAGCGACTG 
GATGATGCCA AACAGAAGCT CCAGCAGACC CTCCAGAGGG CTGAGACGCT GCCCGAGATC 
GAGGCTCAGC TCGCCCAAAG GGTGGCGGCG CTCAATAAGG CGGAGGAGCG GCATGGCAAC 
TTTGAGGAGA GACTGAGACA GCTGGAGGCG CAACTCGAGG AGAAGAACCA GGAGCTGCAG 
AGGGCAAGGC AGAGGGAGCG CATGAATGAT GAACACAATA AACGTCTCTC CGACACGGTG 
GACAAGCTTC TGTCCGAGTC CAATGAGAGG CTTCAGCTGC ACCTGAAGGA GAGAATGGCT 
GCCCTCGAGG AGAAGAACGC TCTTTCTGAG GAACTCGCTA ACATGAAGAA GATCCAAGAC 
GATCTCTTAG CAAACAAGGA TCAACTAATT GCAGAGCTGG AGAGGCTTCA GCTAGAGCTG 
GACCAGCTGA GAGGAAGACC TGGATCCTCT TACTCACGAT CTCTGCCAGG AAGTGCTCTA 
GAGCTGAGGT ACTCTCAAGG AGGCGGGTCA CTTCCTGCCG GCTCCACCAC TCACCTAGAG 
CATTATGGAA ACACCGGCAC AGGGGGTGTG GTCAGACGGA CTCACCGTGG ACGCTGGAGT 
GGTGCCAGGG AGGATGCAAG CAAATATGGC GATTGGGAAA GCGGAGCGCT GCTGGGCTCT 
GGTTTCGAGG GCGGTGTGGA GGACAGGTGT TCTGATGATG AAGAGGACCG CGAGACGCTT 
TTCGGCTCGG AGCTGCTCTC ACCGAGCGGC CAGACAGATG TGCAGACCCT CGCTATCATG 
TTGCAGGAGC AGCTGGAGGC CATCAACAAA GAAATAAAGC TGATTCAGGA AGAGAAGGAG 
AGCACAGAGC TTCGTGCTGA AGAAATCGAG AGCAGAGTCA GCAGCGTGGC TCTGGACGGG 
CCTCCCATTC CACCGACCTC TCTGGGACGG GACAGCACAG GACGGGGCTT CATCCCCCCA 
TCCCTCACAT CCTCCACCCT GGCTTCACCC TCTCCGCCCA GCTCTGGGCA CTCCACACCC 
CGCCTGCCCC ATTCCCCCGC ACGCGAGACA GACAGACAGA ACAACAAAGA GGACGACAGG 
TCTCTGGCCT TACTGGACTC CACCCCTCCT CCCACCCCTC GTGCGCTACG ATTGGACAGG 
ATGACACACA CTCACCCTGG TGCATTGCTG GATGACAGTC GCGAGTTTCG CAGCCTCTCA 
GCTGATGGCA GCAGTTCAAA CAGCAGCCAG GACTCCCTCC ACAAGGCCAG TAAAAAGAAA 
AGCATCAAGT CTTCCATTGG TCGGCTCTTT GGAAAGAAGG ACAAGGTCCG AATGGGTCAG 
CCTGGGCGTG AATCAACATC TCTGGCCTCA ACCCCTTCAG ATGACTTGGG CACTGTGGAT 
CCCCTCGGAC TCACTAAGAT GGCCGGTCCG GTAGACAAGG ACCGGCGCAG CAAGAAGAAG 
CATGAGCTGC TGGAGGAAGC ATGTCGACAG GGTCTGCCCT TTGCATCCTG GGATGGACCC 
ACTGTTGTCT CCTGGCTGGA ACTGTGGGTG GGCATGCCGG CGTGGTATGT AGCAGCATGT 
CGTGCAAACG TGAAGAGTGG AGCCATCATG GCAAACCTGT CTGACACAGA GATCCAGAGA 
GAGATCGGCA TCAGTAATCC TCTGCACCGC CTCAAACTGA GACTGGCCAT TCAAGAGATG 
GTGTCCCTGA CCAGCCCCTC AGCCCCGGCT AGCACACGCT CCTCCACCAG TAACGTGTGG 
ATGACCCACG CTGAGATGGA GTCCCTGACT GCAGCAACCA AACCAGAGCT GAAAGAGATT 
AGCTGGGATC AGATCCTGGC CTATGGAGAC ATGAACCATG AGTGGGTGGG CAATGACTGG 
CTGCCCAGTC TGGGTTTGCC CCAGTACAGA AGCTACTTCA TGGAGTCTCT TGTGGATGCC 
CGCATGTTGG ACCATCTGAC CAAGAAAGAG CTGCGTGGCC AGCTGAAAAT GGTGGACAGT 
TTCCACAGGG TGAGCATGCA TTATGGCATC ATGTGCCTGA AGCGCCTCAA TTATGACCGC 
AAGGAGCTGG AGAGGAGGAG AGATGAGAGC CAGCACCAGA ATAAAGATGT GATGGTGTGG 
TCGAACGAGC GTGTGATGTG CTGGGTTCAG TCCATCGGCT TGAAGGAGTA CGCAGATAAC 
CTGACTGAGA GCGGTGTTCA TGGAGCGCTG TTGGCCCTGG ATGACACCTT CGATTACACT 
GACCTCGCAC TGCTCCTGCA GATTCCCAAT CAAAACACAC AGGCACGACA ACTTCTGGAG 
AAGGAATACA ACTCCCTCAT CTCAATGGGA ACAGAAAGGA GGCCAGACGA GGATGGCACT 
AAGACGTTCA CCCGCTCTCC TTCATGGAGG AAGATGTTTC GGGAGAAGGA CCTGCGGGGT 
GTGACGTCTG ACTCCTCCGA GACCCTGCCT GCTAACTTCC GTGCCTCAGC CATCTCTACC 
CCCTCAGTCA CGCTGAGGAA GGTCCAGAGC GATGCATCTC CAAGGGCAGA GTCTGGGTCA 
GTGAGGACCT ACTCCTGTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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ppfia3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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