PDE3A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	PDE3A
Entrez Gene ID	567051
Full Name	phosphodiesterase 3A, cGMP-inhibited
Synonyms	si:dkey-48h7.2
General protein information	Preferred Names phosphodiesterase 3A, cGMP-inhibited Names cGMP-inhibited 3',5'-cyclic phosphodiesterase A
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009299994.3	XP_009298269.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X1
XM_009299995.3	XP_009298270.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X2
XM_009299997.2	XP_009298272.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X4
XM_009299996.3	XP_009298271.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X3
XM_009299997.3	XP_009298272.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X4
XM_009299994.2	XP_009298269.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X1
XM_009299995.2	XP_009298270.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X2
XM_009299996.2	XP_009298271.1	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X3

Pathways from BioSystems

Homologies

	PDE3A	XP_001096963.2
Canis lupus familiaris (dog)	PDE3A	XP_005637137.1
Rattus norvegicus (Norway rat)	Pde3a	NP_059033.1
Danio rerio (zebrafish)	pde3a	XP_695432.4
Pan troglodytes (chimpanzee)	PDE3A	XP_520783.2
Mus musculus (house mouse)	Pde3a	NP_061249.1
Gallus gallus (chicken)	PDE3A	XP_416416.4
Homo sapiens (human)	PDE3A	NP_000912.3
Bos taurus (cattle)	PDE3A	XP_002687798.1

Gene Ontology

The following PDE3A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDE3A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa45058	XM_009299994.3 Latest version!	Danio rerio phosphodiesterase 3A, cGMP-inhibited (pde3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa45059	XM_009299995.3 Latest version!	Danio rerio phosphodiesterase 3A, cGMP-inhibited (pde3a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa45060	XM_009299996.3 Latest version!	Danio rerio phosphodiesterase 3A, cGMP-inhibited (pde3a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa45061	XM_009299997.3 Latest version!	Danio rerio phosphodiesterase 3A, cGMP-inhibited (pde3a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa45061	XM_009299997.2	Danio rerio phosphodiesterase 3A, cGMP-inhibited (pde3a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa45058	XM_009299994.2	Danio rerio phosphodiesterase 3A, cGMP-inhibited (pde3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa45059	XM_009299995.2	Danio rerio phosphodiesterase 3A, cGMP-inhibited (pde3a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa45060	XM_009299996.2	Danio rerio phosphodiesterase 3A, cGMP-inhibited (pde3a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDE3A ( XM_009299994.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009298269.1 Danio rerio phosphodiesterase 3A, cGMP-inhibited (pde3a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa45058
Clone ID Related Accession (Same CDS sequence)	XM_009299994.3 , XM_009299994.2
Accession Version	XM_009299994.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299994.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG AATCCGTGCG CAACGGCTAT GTGCGCACTT GTGTCACTCC CGTGGAGCAG 
GACTGCTGGC ACTCGTGCTT GCGAAGCCGT AGAAGCCTCT CGTCGGCCGT GTGCGTCGCC 
TCGCTGTCCG TGCTCCTCGC GCTGCTCTGG GACGCGGAGC GCACACACGC GCACTCGGGC 
TCTCACTTGG TGACGCTCGT GGAGCTCGTG TGGCAGCTGC TCTCGCCCGT CCTCACGCTC 
GTGTGTGCCT TCTTCTGGGT GGGTGTGTAT CTGCTCCGCT GCGGGGTGCT GATCCGCACG 
GCAGTGTTCC TGCTCACCGT GTGCCATCTG GGCGAAGCGG CGGCGCAGTC GCTGCTGGAT 
GCGCAGCAGC TGTACTCATT CACGGCCACC GCGCTGGTGC TGCTCGCCTG CCTGGCCAGC 
GGGGCGCTCA TGGTGGTGCG GCTGGGTCAG GGCATCTCCG TGCTGCTCTT CATCAGCATC 
ATCAGGACCG TGTCGCTTCT GTCGCTGCAG AAAGTGCGCG CCAGCTGGAG ACCGTACCTG 
GCGTACCTGC TCGGGCTGCT CGGGGTCCTG CTGGCGAGGT ATGCGGACAG GCTGCTGCCG 
GAGCAGGGCG CATCACACCG GGAGGGATCG AGGGGAGATA TTCCGGTATT TAAGAGGCGC 
AGGAGGTCCA GCTCCGTAGT CTCGTCAGAC ATGGCACAGC ACGGCCAGTC CAGAAGCAAA 
TCGCACAGGA GGACATCTCT GCCCTGCCTG CAGAGAGAAC AGATGCTCCC ACAGCAAGAA 
TGGGACAACA AAAGAGGCGC CCGAGGATCT CAGCAGCCAT CCGGCCTCAT GCAATCTTCG 
GGCACCAGTG TGACGGTGGA TATCGCGGTG ATGGGCGAGG CTCACGGACT GATCTCAGAC 
CTGCTGGCTG ACCAGTCGCT CGCACCCAAC ATCTGCAGCA CCCTGCGGGC CGTCAGTAAC 
CTGCTGAACA CCCAGCTCAC CTTCCAGCCC CTGCACCACC GGCCACGCCT GGGCCCCCTG 
CTGGCCTTCA GCGACGGACA CACGTGCTCC GACTCGGAGG AGGCGCCTGA GAAAGGAGAG 
AGGCTGGCTA TTCCAAAGCG TTTGAGGAGG AGTTTGCATC CAGGGCTTTT GAGACGGATT 
TCTTCCACGT GGACCACCAC CACCTCCGCC ACAGGCCTGC CAACCCTCGA ACCCGGGCCG 
GTCCGACGAG ACCGCAGCGC CAGCATCAAA GCACCTCACG ATGGCCCTTC CAGCAGTTTG 
GTGAACAGTG ATTCATGGAA CAACTCCAAT ATGCTCAGCA TTTCCAAGAG CAGGTCCATG 
TCTGCTTCAT ACGCTGCTTC CTCCAACACC ACCAACCACA TCTATAGCAA GAGCCGGAGA 
GGTTGCTCTT CTTCCACCAT CTCTCCTCTG GCGTCCCCCT GTCACTCTCC TCCAGTGCAG 
AGCTCTCCGC TGACCAGCCC CACCAACAAG ACCCCCTCAG TGGAGCTGCC AGACCCCGAG 
CTTCCTCCAC CACGCAGCCC ACATAAAGCC TTAACCCACA GTCAGAGCGC ACCCAGCTCC 
AGCGCAGCGC TCTGGCCACC GCCAACGCCA CTATGTAGCA GCTGTGGACG GCCGTACAGT 
AAACAGAGGC ACAGTGATGG ATCCACAGAG CCAGCAGAGC GAACACAACA GTCAGACGAG 
CCGGCGATGA TGTCATCAGA TTATGACAGC ACGTATGAAA CCAACCACAG TGACAGCAGC 
GACTTTGCAC AGACTGACGA GGACAGAGAG GCTCGCAGGA CGCTAACGCT AGCCACGCTT 
ATACCAGCCG AGGAGAAGCC CATCTTGGCC CCGGAGCCGC TGCTGATGGA GGGACTGGAG 
CCGCTTCTGT GTCAGATCAA CAACTGGAAC TTTCCCATCT TCAGCCTGGT GGAGAAAACC 
AACGGCAGAT GTGGCTGCAT CCTCAGTCAG ATGTCCTACC GACTGTTTGT CGACACGGGA 
CTCTTTGAGA CATTCAAGAT TCCAGTGCGT GAGTTCATGA ACTATTTCCA CGCGCTGGAG 
AATGGATACA GGGACATCCC ATATCACAAC AGAATTCATG CCACAGACGT CCTCCATGCA 
GTCTGGTACC TCACTACACA ACCTGTGCCT GGTTTACCCA CACTGCGGAC ACTCGGCAGC 
TCCGACTCTG ACAGTGGACT CGCTCACGGC CGGATGAAGT ATCTGATGTC CAGGACTAAT 
GCAGTGGAGG AGGAAGGTTA CGGCTGTCTT TCAGGCCTCA TTCCTGCGCT GGAGCTCATG 
GCGCTGTACG TCGCTGCTGC AATGCATGAT TATGACCATC CTGGTCGGAC CAACGCCTTC 
CTGGTGGCCA CCAGCGCTCC ACAGGCTGTG CTATACAATG ACCGTTCAGT GCTGGAGAAT 
CATCACGCTG CCTCCGCCTG GAACCTCTTC ATGTCGCGGC CCGAGTACAA CTTTCTGGCC 
AGTTTGGACC ACATGGAGTT TAAACGCTTT CGCTTCCTGG TCATCGAAGC CATTCTGGCC 
ACTGACCTCA AGAAACACTT TGATTTCCTG GCCGAATTCA ACGCCAAGGT GGGTGATGAC 
GGCTGCTCAG GGATCGACTG GACCAATGAG AACGACCGTC TGCTGGTCTG TCAGATGTGC 
ATTAAGCTAG CAGACATAAA CGGGCCGCTG AAATGCAAGG ATCTTCATTT GCAGTGGACG 
GAGGGCATCG TCAATGAGTT TTACGAGCAG GGTGATGAAG AGTCCAGTCT CGGTCTTCCC 
ATCAGCCCCT TCATGGACCG CTCAGCACCT CAGCTGGCCA AACTGCAAGA GTCCTTCATC 
ACACACATCG TGGGTCCTCT GTGCAGCTCG TATGACTCTG CGGCGCTCAT GCCTGGCCGC 
TGGGTCCACC CTCTGCCCAA AGATGGAGAT GAGGTGGAGG ACGAGAGCGA GGAGGAGGAT 
GAGGACACAG CGGAGGAGGA GGAAGAGGAC GCCTCCACCA GCTCTGAACT CTCCCAAAAA 
CCAGCACCTA AGAAACGAAG GAAAGTCTTC TGCCAGATCT CCCATCACCT GCTGGAGAAC 
CATGAGATGT GGAAGAAGGT CATTGCAGAG GAGTCCCAGA GCCAGACTGA AGGGGAGGAG 
TCAACATGCC TGAGCACCAC CCCTGAGCCA ATCCTGGCCA TCGATGAAGA GGAGGAGGAG 
CCAGGGAGCA AGGAGGAGCC AACGGATGAC CAAGGGGAGG GGTCAACAGA GGAAGAGGAG 
GGACCCGTAC CTGAAGACAC GCATGAAACA CAGGAACCAG ATGGCGAGAC TGTAGAGGAA 
GCAGATGAGA AAAACACAGA GACTGTAGAA GACATTGAGG GAAAACAAGA CGAAGACCAG 
AAAGATCCAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298269.1
CDS	646..4020
Translation	MALESVRNGYVRTCVTPVEQDCWHSCLRSRRSLSSAVCVASLSVLLALLWDAERTHAHSGSHLVTLVELVWQLLSPVLTLVCAFFWVGVYLLRCGVLIRTAVFLLTVCHLGEAAAQSLLDAQQLYSFTATALVLLACLASGALMVVRLGQGISVLLFISIIRTVSLLSLQKVRASWRPYLAYLLGLLGVLLARYADRLLPEQGASHREGSRGDIPVFKRRRRSSSVVSSDMAQHGQSRSKSHRRTSLPCLQREQMLPQQEWDNKRGARGSQQPSGLMQSSGTSVTVDIAVMGEAHGLISDLLADQSLAPNICSTLRAVSNLLNTQLTFQPLHHRPRLGPLLAFSDGHTCSDSEEAPEKGERLAIPKRLRRSLHPGLLRRISSTWTTTTSATGLPTLEPGPVRRDRSASIKAPHDGPSSSLVNSDSWNNSNMLSISKSRSMSASYAASSNTTNHIYSKSRRGCSSSTISPLASPCHSPPVQSSPLTSPTNKTPSVELPDPELPPPRSPHKALTHSQSAPSSSAALWPPPTPLCSSCGRPYSKQRHSDGSTEPAERTQQSDEPAMMSSDYDSTYETNHSDSSDFAQTDEDREARRTLTLATLIPAEEKPILAPEPLLMEGLEPLLCQINNWNFPIFSLVEKTNGRCGCILSQMSYRLFVDTGLFETFKIPVREFMNYFHALENGYRDIPYHNRIHATDVLHAVWYLTTQPVPGLPTLRTLGSSDSDSGLAHGRMKYLMSRTNAVEEEGYGCLSGLIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQAVLYNDRSVLENHHAASAWNLFMSRPEYNFLASLDHMEFKRFRFLVIEAILATDLKKHFDFLAEFNAKVGDDGCSGIDWTNENDRLLVCQMCIKLADINGPLKCKDLHLQWTEGIVNEFYEQGDEESSLGLPISPFMDRSAPQLAKLQESFITHIVGPLCSSYDSAALMPGRWVHPLPKDGDEVEDESEEEDEDTAEEEEEDASTSSELSQKPAPKKRRKVFCQISHHLLENHEMWKKVIAEESQSQTEGEESTCLSTTPEPILAIDEEEEEPGSKEEPTDDQGEGSTEEEEGPVPEDTHETQEPDGETVEEADEKNTETVEDIEGKQDEDQKDPE

Target ORF information:

RefSeq Version	XM_009299994.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphodiesterase 3A, cGMP-inhibited (pde3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299994.3

ORF Insert Sequence:

ATGGCACTGG AATCCGTGCG CAACGGCTAT GTGCGCACTT GTGTCACTCC CGTGGAGCAG 
GACTGCTGGC ACTCGTGCTT GCGAAGCCGT AGAAGCCTCT CGTCGGCCGT GTGCGTCGCC 
TCGCTGTCCG TGCTCCTCGC GCTGCTCTGG GACGCGGAGC GCACACACGC GCACTCGGGC 
TCTCACTTGG TGACGCTCGT GGAGCTCGTG TGGCAGCTGC TCTCGCCCGT CCTCACGCTC 
GTGTGTGCCT TCTTCTGGGT GGGTGTGTAT CTGCTCCGCT GCGGGGTGCT GATCCGCACG 
GCAGTGTTCC TGCTCACCGT GTGCCATCTG GGCGAAGCGG CGGCGCAGTC GCTGCTGGAT 
GCGCAGCAGC TGTACTCATT CACGGCCACC GCGCTGGTGC TGCTCGCCTG CCTGGCCAGC 
GGGGCGCTCA TGGTGGTGCG GCTGGGTCAG GGCATCTCCG TGCTGCTCTT CATCAGCATC 
ATCAGGACCG TGTCGCTTCT GTCGCTGCAG AAAGTGCGCG CCAGCTGGAG ACCGTACCTG 
GCGTACCTGC TCGGGCTGCT CGGGGTCCTG CTGGCGAGGT ATGCGGACAG GCTGCTGCCG 
GAGCAGGGCG CATCACACCG GGAGGGATCG AGGGGAGATA TTCCGGTATT TAAGAGGCGC 
AGGAGGTCCA GCTCCGTAGT CTCGTCAGAC ATGGCACAGC ACGGCCAGTC CAGAAGCAAA 
TCGCACAGGA GGACATCTCT GCCCTGCCTG CAGAGAGAAC AGATGCTCCC ACAGCAAGAA 
TGGGACAACA AAAGAGGCGC CCGAGGATCT CAGCAGCCAT CCGGCCTCAT GCAATCTTCG 
GGCACCAGTG TGACGGTGGA TATCGCGGTG ATGGGCGAGG CTCACGGACT GATCTCAGAC 
CTGCTGGCTG ACCAGTCGCT CGCACCCAAC ATCTGCAGCA CCCTGCGGGC CGTCAGTAAC 
CTGCTGAACA CCCAGCTCAC CTTCCAGCCC CTGCACCACC GGCCACGCCT GGGCCCCCTG 
CTGGCCTTCA GCGACGGACA CACGTGCTCC GACTCGGAGG AGGCGCCTGA GAAAGGAGAG 
AGGCTGGCTA TTCCAAAGCG TTTGAGGAGG AGTTTGCATC CAGGGCTTTT GAGACGGATT 
TCTTCCACGT GGACCACCAC CACCTCCGCC ACAGGCCTGC CAACCCTCGA ACCCGGGCCG 
GTCCGACGAG ACCGCAGCGC CAGCATCAAA GCACCTCACG ATGGCCCTTC CAGCAGTTTG 
GTGAACAGTG ATTCATGGAA CAACTCCAAT ATGCTCAGCA TTTCCAAGAG CAGGTCCATG 
TCTGCTTCAT ACGCTGCTTC CTCCAACACC ACCAACCACA TCTATAGCAA GAGCCGGAGA 
GGTTGCTCTT CTTCCACCAT CTCTCCTCTG GCGTCCCCCT GTCACTCTCC TCCAGTGCAG 
AGCTCTCCGC TGACCAGCCC CACCAACAAG ACCCCCTCAG TGGAGCTGCC AGACCCCGAG 
CTTCCTCCAC CACGCAGCCC ACATAAAGCC TTAACCCACA GTCAGAGCGC ACCCAGCTCC 
AGCGCAGCGC TCTGGCCACC GCCAACGCCA CTATGTAGCA GCTGTGGACG GCCGTACAGT 
AAACAGAGGC ACAGTGATGG ATCCACAGAG CCAGCAGAGC GAACACAACA GTCAGACGAG 
CCGGCGATGA TGTCATCAGA TTATGACAGC ACGTATGAAA CCAACCACAG TGACAGCAGC 
GACTTTGCAC AGACTGACGA GGACAGAGAG GCTCGCAGGA CGCTAACGCT AGCCACGCTT 
ATACCAGCCG AGGAGAAGCC CATCTTGGCC CCGGAGCCGC TGCTGATGGA GGGACTGGAG 
CCGCTTCTGT GTCAGATCAA CAACTGGAAC TTTCCCATCT TCAGCCTGGT GGAGAAAACC 
AACGGCAGAT GTGGCTGCAT CCTCAGTCAG ATGTCCTACC GACTGTTTGT CGACACGGGA 
CTCTTTGAGA CATTCAAGAT TCCAGTGCGT GAGTTCATGA ACTATTTCCA CGCGCTGGAG 
AATGGATACA GGGACATCCC ATATCACAAC AGAATTCATG CCACAGACGT CCTCCATGCA 
GTCTGGTACC TCACTACACA ACCTGTGCCT GGTTTACCCA CACTGCGGAC ACTCGGCAGC 
TCCGACTCTG ACAGTGGACT CGCTCACGGC CGGATGAAGT ATCTGATGTC CAGGACTAAT 
GCAGTGGAGG AGGAAGGTTA CGGCTGTCTT TCAGGCCTCA TTCCTGCGCT GGAGCTCATG 
GCGCTGTACG TCGCTGCTGC AATGCATGAT TATGACCATC CTGGTCGGAC CAACGCCTTC 
CTGGTGGCCA CCAGCGCTCC ACAGGCTGTG CTATACAATG ACCGTTCAGT GCTGGAGAAT 
CATCACGCTG CCTCCGCCTG GAACCTCTTC ATGTCGCGGC CCGAGTACAA CTTTCTGGCC 
AGTTTGGACC ACATGGAGTT TAAACGCTTT CGCTTCCTGG TCATCGAAGC CATTCTGGCC 
ACTGACCTCA AGAAACACTT TGATTTCCTG GCCGAATTCA ACGCCAAGGT GGGTGATGAC 
GGCTGCTCAG GGATCGACTG GACCAATGAG AACGACCGTC TGCTGGTCTG TCAGATGTGC 
ATTAAGCTAG CAGACATAAA CGGGCCGCTG AAATGCAAGG ATCTTCATTT GCAGTGGACG 
GAGGGCATCG TCAATGAGTT TTACGAGCAG GGTGATGAAG AGTCCAGTCT CGGTCTTCCC 
ATCAGCCCCT TCATGGACCG CTCAGCACCT CAGCTGGCCA AACTGCAAGA GTCCTTCATC 
ACACACATCG TGGGTCCTCT GTGCAGCTCG TATGACTCTG CGGCGCTCAT GCCTGGCCGC 
TGGGTCCACC CTCTGCCCAA AGATGGAGAT GAGGTGGAGG ACGAGAGCGA GGAGGAGGAT 
GAGGACACAG CGGAGGAGGA GGAAGAGGAC GCCTCCACCA GCTCTGAACT CTCCCAAAAA 
CCAGCACCTA AGAAACGAAG GAAAGTCTTC TGCCAGATCT CCCATCACCT GCTGGAGAAC 
CATGAGATGT GGAAGAAGGT CATTGCAGAG GAGTCCCAGA GCCAGACTGA AGGGGAGGAG 
TCAACATGCC TGAGCACCAC CCCTGAGCCA ATCCTGGCCA TCGATGAAGA GGAGGAGGAG 
CCAGGGAGCA AGGAGGAGCC AACGGATGAC CAAGGGGAGG GGTCAACAGA GGAAGAGGAG 
GGACCCGTAC CTGAAGACAC GCATGAAACA CAGGAACCAG ATGGCGAGAC TGTAGAGGAA 
GCAGATGAGA AAAACACAGA GACTGTAGAA GACATTGAGG GAAAACAAGA CGAAGACCAG 
AAAGATCCAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE3A ( XM_009299994.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009298269.1 Danio rerio phosphodiesterase 3A, cGMP-inhibited (pde3a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa45058
Clone ID Related Accession (Same CDS sequence)	XM_009299994.3 , XM_009299994.2
Accession Version	XM_009299994.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299994.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG AATCCGTGCG CAACGGCTAT GTGCGCACTT GTGTCACTCC CGTGGAGCAG 
GACTGCTGGC ACTCGTGCTT GCGAAGCCGT AGAAGCCTCT CGTCGGCCGT GTGCGTCGCC 
TCGCTGTCCG TGCTCCTCGC GCTGCTCTGG GACGCGGAGC GCACACACGC GCACTCGGGC 
TCTCACTTGG TGACGCTCGT GGAGCTCGTG TGGCAGCTGC TCTCGCCCGT CCTCACGCTC 
GTGTGTGCCT TCTTCTGGGT GGGTGTGTAT CTGCTCCGCT GCGGGGTGCT GATCCGCACG 
GCAGTGTTCC TGCTCACCGT GTGCCATCTG GGCGAAGCGG CGGCGCAGTC GCTGCTGGAT 
GCGCAGCAGC TGTACTCATT CACGGCCACC GCGCTGGTGC TGCTCGCCTG CCTGGCCAGC 
GGGGCGCTCA TGGTGGTGCG GCTGGGTCAG GGCATCTCCG TGCTGCTCTT CATCAGCATC 
ATCAGGACCG TGTCGCTTCT GTCGCTGCAG AAAGTGCGCG CCAGCTGGAG ACCGTACCTG 
GCGTACCTGC TCGGGCTGCT CGGGGTCCTG CTGGCGAGGT ATGCGGACAG GCTGCTGCCG 
GAGCAGGGCG CATCACACCG GGAGGGATCG AGGGGAGATA TTCCGGTATT TAAGAGGCGC 
AGGAGGTCCA GCTCCGTAGT CTCGTCAGAC ATGGCACAGC ACGGCCAGTC CAGAAGCAAA 
TCGCACAGGA GGACATCTCT GCCCTGCCTG CAGAGAGAAC AGATGCTCCC ACAGCAAGAA 
TGGGACAACA AAAGAGGCGC CCGAGGATCT CAGCAGCCAT CCGGCCTCAT GCAATCTTCG 
GGCACCAGTG TGACGGTGGA TATCGCGGTG ATGGGCGAGG CTCACGGACT GATCTCAGAC 
CTGCTGGCTG ACCAGTCGCT CGCACCCAAC ATCTGCAGCA CCCTGCGGGC CGTCAGTAAC 
CTGCTGAACA CCCAGCTCAC CTTCCAGCCC CTGCACCACC GGCCACGCCT GGGCCCCCTG 
CTGGCCTTCA GCGACGGACA CACGTGCTCC GACTCGGAGG AGGCGCCTGA GAAAGGAGAG 
AGGCTGGCTA TTCCAAAGCG TTTGAGGAGG AGTTTGCATC CAGGGCTTTT GAGACGGATT 
TCTTCCACGT GGACCACCAC CACCTCCGCC ACAGGCCTGC CAACCCTCGA ACCCGGGCCG 
GTCCGACGAG ACCGCAGCGC CAGCATCAAA GCACCTCACG ATGGCCCTTC CAGCAGTTTG 
GTGAACAGTG ATTCATGGAA CAACTCCAAT ATGCTCAGCA TTTCCAAGAG CAGGTCCATG 
TCTGCTTCAT ACGCTGCTTC CTCCAACACC ACCAACCACA TCTATAGCAA GAGCCGGAGA 
GGTTGCTCTT CTTCCACCAT CTCTCCTCTG GCGTCCCCCT GTCACTCTCC TCCAGTGCAG 
AGCTCTCCGC TGACCAGCCC CACCAACAAG ACCCCCTCAG TGGAGCTGCC AGACCCCGAG 
CTTCCTCCAC CACGCAGCCC ACATAAAGCC TTAACCCACA GTCAGAGCGC ACCCAGCTCC 
AGCGCAGCGC TCTGGCCACC GCCAACGCCA CTATGTAGCA GCTGTGGACG GCCGTACAGT 
AAACAGAGGC ACAGTGATGG ATCCACAGAG CCAGCAGAGC GAACACAACA GTCAGACGAG 
CCGGCGATGA TGTCATCAGA TTATGACAGC ACGTATGAAA CCAACCACAG TGACAGCAGC 
GACTTTGCAC AGACTGACGA GGACAGAGAG GCTCGCAGGA CGCTAACGCT AGCCACGCTT 
ATACCAGCCG AGGAGAAGCC CATCTTGGCC CCGGAGCCGC TGCTGATGGA GGGACTGGAG 
CCGCTTCTGT GTCAGATCAA CAACTGGAAC TTTCCCATCT TCAGCCTGGT GGAGAAAACC 
AACGGCAGAT GTGGCTGCAT CCTCAGTCAG ATGTCCTACC GACTGTTTGT CGACACGGGA 
CTCTTTGAGA CATTCAAGAT TCCAGTGCGT GAGTTCATGA ACTATTTCCA CGCGCTGGAG 
AATGGATACA GGGACATCCC ATATCACAAC AGAATTCATG CCACAGACGT CCTCCATGCA 
GTCTGGTACC TCACTACACA ACCTGTGCCT GGTTTACCCA CACTGCGGAC ACTCGGCAGC 
TCCGACTCTG ACAGTGGACT CGCTCACGGC CGGATGAAGT ATCTGATGTC CAGGACTAAT 
GCAGTGGAGG AGGAAGGTTA CGGCTGTCTT TCAGGCCTCA TTCCTGCGCT GGAGCTCATG 
GCGCTGTACG TCGCTGCTGC AATGCATGAT TATGACCATC CTGGTCGGAC CAACGCCTTC 
CTGGTGGCCA CCAGCGCTCC ACAGGCTGTG CTATACAATG ACCGTTCAGT GCTGGAGAAT 
CATCACGCTG CCTCCGCCTG GAACCTCTTC ATGTCGCGGC CCGAGTACAA CTTTCTGGCC 
AGTTTGGACC ACATGGAGTT TAAACGCTTT CGCTTCCTGG TCATCGAAGC CATTCTGGCC 
ACTGACCTCA AGAAACACTT TGATTTCCTG GCCGAATTCA ACGCCAAGGT GGGTGATGAC 
GGCTGCTCAG GGATCGACTG GACCAATGAG AACGACCGTC TGCTGGTCTG TCAGATGTGC 
ATTAAGCTAG CAGACATAAA CGGGCCGCTG AAATGCAAGG ATCTTCATTT GCAGTGGACG 
GAGGGCATCG TCAATGAGTT TTACGAGCAG GGTGATGAAG AGTCCAGTCT CGGTCTTCCC 
ATCAGCCCCT TCATGGACCG CTCAGCACCT CAGCTGGCCA AACTGCAAGA GTCCTTCATC 
ACACACATCG TGGGTCCTCT GTGCAGCTCG TATGACTCTG CGGCGCTCAT GCCTGGCCGC 
TGGGTCCACC CTCTGCCCAA AGATGGAGAT GAGGTGGAGG ACGAGAGCGA GGAGGAGGAT 
GAGGACACAG CGGAGGAGGA GGAAGAGGAC GCCTCCACCA GCTCTGAACT CTCCCAAAAA 
CCAGCACCTA AGAAACGAAG GAAAGTCTTC TGCCAGATCT CCCATCACCT GCTGGAGAAC 
CATGAGATGT GGAAGAAGGT CATTGCAGAG GAGTCCCAGA GCCAGACTGA AGGGGAGGAG 
TCAACATGCC TGAGCACCAC CCCTGAGCCA ATCCTGGCCA TCGATGAAGA GGAGGAGGAG 
CCAGGGAGCA AGGAGGAGCC AACGGATGAC CAAGGGGAGG GGTCAACAGA GGAAGAGGAG 
GGACCCGTAC CTGAAGACAC GCATGAAACA CAGGAACCAG ATGGCGAGAC TGTAGAGGAA 
GCAGATGAGA AAAACACAGA GACTGTAGAA GACATTGAGG GAAAACAAGA CGAAGACCAG 
AAAGATCCAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298269.1
CDS	646..4020
Translation	MALESVRNGYVRTCVTPVEQDCWHSCLRSRRSLSSAVCVASLSVLLALLWDAERTHAHSGSHLVTLVELVWQLLSPVLTLVCAFFWVGVYLLRCGVLIRTAVFLLTVCHLGEAAAQSLLDAQQLYSFTATALVLLACLASGALMVVRLGQGISVLLFISIIRTVSLLSLQKVRASWRPYLAYLLGLLGVLLARYADRLLPEQGASHREGSRGDIPVFKRRRRSSSVVSSDMAQHGQSRSKSHRRTSLPCLQREQMLPQQEWDNKRGARGSQQPSGLMQSSGTSVTVDIAVMGEAHGLISDLLADQSLAPNICSTLRAVSNLLNTQLTFQPLHHRPRLGPLLAFSDGHTCSDSEEAPEKGERLAIPKRLRRSLHPGLLRRISSTWTTTTSATGLPTLEPGPVRRDRSASIKAPHDGPSSSLVNSDSWNNSNMLSISKSRSMSASYAASSNTTNHIYSKSRRGCSSSTISPLASPCHSPPVQSSPLTSPTNKTPSVELPDPELPPPRSPHKALTHSQSAPSSSAALWPPPTPLCSSCGRPYSKQRHSDGSTEPAERTQQSDEPAMMSSDYDSTYETNHSDSSDFAQTDEDREARRTLTLATLIPAEEKPILAPEPLLMEGLEPLLCQINNWNFPIFSLVEKTNGRCGCILSQMSYRLFVDTGLFETFKIPVREFMNYFHALENGYRDIPYHNRIHATDVLHAVWYLTTQPVPGLPTLRTLGSSDSDSGLAHGRMKYLMSRTNAVEEEGYGCLSGLIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQAVLYNDRSVLENHHAASAWNLFMSRPEYNFLASLDHMEFKRFRFLVIEAILATDLKKHFDFLAEFNAKVGDDGCSGIDWTNENDRLLVCQMCIKLADINGPLKCKDLHLQWTEGIVNEFYEQGDEESSLGLPISPFMDRSAPQLAKLQESFITHIVGPLCSSYDSAALMPGRWVHPLPKDGDEVEDESEEEDEDTAEEEEEDASTSSELSQKPAPKKRRKVFCQISHHLLENHEMWKKVIAEESQSQTEGEESTCLSTTPEPILAIDEEEEEPGSKEEPTDDQGEGSTEEEEGPVPEDTHETQEPDGETVEEADEKNTETVEDIEGKQDEDQKDPE

Target ORF information:

RefSeq Version	XM_009299994.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphodiesterase 3A, cGMP-inhibited (pde3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299994.2

ORF Insert Sequence:

ATGGCACTGG AATCCGTGCG CAACGGCTAT GTGCGCACTT GTGTCACTCC CGTGGAGCAG 
GACTGCTGGC ACTCGTGCTT GCGAAGCCGT AGAAGCCTCT CGTCGGCCGT GTGCGTCGCC 
TCGCTGTCCG TGCTCCTCGC GCTGCTCTGG GACGCGGAGC GCACACACGC GCACTCGGGC 
TCTCACTTGG TGACGCTCGT GGAGCTCGTG TGGCAGCTGC TCTCGCCCGT CCTCACGCTC 
GTGTGTGCCT TCTTCTGGGT GGGTGTGTAT CTGCTCCGCT GCGGGGTGCT GATCCGCACG 
GCAGTGTTCC TGCTCACCGT GTGCCATCTG GGCGAAGCGG CGGCGCAGTC GCTGCTGGAT 
GCGCAGCAGC TGTACTCATT CACGGCCACC GCGCTGGTGC TGCTCGCCTG CCTGGCCAGC 
GGGGCGCTCA TGGTGGTGCG GCTGGGTCAG GGCATCTCCG TGCTGCTCTT CATCAGCATC 
ATCAGGACCG TGTCGCTTCT GTCGCTGCAG AAAGTGCGCG CCAGCTGGAG ACCGTACCTG 
GCGTACCTGC TCGGGCTGCT CGGGGTCCTG CTGGCGAGGT ATGCGGACAG GCTGCTGCCG 
GAGCAGGGCG CATCACACCG GGAGGGATCG AGGGGAGATA TTCCGGTATT TAAGAGGCGC 
AGGAGGTCCA GCTCCGTAGT CTCGTCAGAC ATGGCACAGC ACGGCCAGTC CAGAAGCAAA 
TCGCACAGGA GGACATCTCT GCCCTGCCTG CAGAGAGAAC AGATGCTCCC ACAGCAAGAA 
TGGGACAACA AAAGAGGCGC CCGAGGATCT CAGCAGCCAT CCGGCCTCAT GCAATCTTCG 
GGCACCAGTG TGACGGTGGA TATCGCGGTG ATGGGCGAGG CTCACGGACT GATCTCAGAC 
CTGCTGGCTG ACCAGTCGCT CGCACCCAAC ATCTGCAGCA CCCTGCGGGC CGTCAGTAAC 
CTGCTGAACA CCCAGCTCAC CTTCCAGCCC CTGCACCACC GGCCACGCCT GGGCCCCCTG 
CTGGCCTTCA GCGACGGACA CACGTGCTCC GACTCGGAGG AGGCGCCTGA GAAAGGAGAG 
AGGCTGGCTA TTCCAAAGCG TTTGAGGAGG AGTTTGCATC CAGGGCTTTT GAGACGGATT 
TCTTCCACGT GGACCACCAC CACCTCCGCC ACAGGCCTGC CAACCCTCGA ACCCGGGCCG 
GTCCGACGAG ACCGCAGCGC CAGCATCAAA GCACCTCACG ATGGCCCTTC CAGCAGTTTG 
GTGAACAGTG ATTCATGGAA CAACTCCAAT ATGCTCAGCA TTTCCAAGAG CAGGTCCATG 
TCTGCTTCAT ACGCTGCTTC CTCCAACACC ACCAACCACA TCTATAGCAA GAGCCGGAGA 
GGTTGCTCTT CTTCCACCAT CTCTCCTCTG GCGTCCCCCT GTCACTCTCC TCCAGTGCAG 
AGCTCTCCGC TGACCAGCCC CACCAACAAG ACCCCCTCAG TGGAGCTGCC AGACCCCGAG 
CTTCCTCCAC CACGCAGCCC ACATAAAGCC TTAACCCACA GTCAGAGCGC ACCCAGCTCC 
AGCGCAGCGC TCTGGCCACC GCCAACGCCA CTATGTAGCA GCTGTGGACG GCCGTACAGT 
AAACAGAGGC ACAGTGATGG ATCCACAGAG CCAGCAGAGC GAACACAACA GTCAGACGAG 
CCGGCGATGA TGTCATCAGA TTATGACAGC ACGTATGAAA CCAACCACAG TGACAGCAGC 
GACTTTGCAC AGACTGACGA GGACAGAGAG GCTCGCAGGA CGCTAACGCT AGCCACGCTT 
ATACCAGCCG AGGAGAAGCC CATCTTGGCC CCGGAGCCGC TGCTGATGGA GGGACTGGAG 
CCGCTTCTGT GTCAGATCAA CAACTGGAAC TTTCCCATCT TCAGCCTGGT GGAGAAAACC 
AACGGCAGAT GTGGCTGCAT CCTCAGTCAG ATGTCCTACC GACTGTTTGT CGACACGGGA 
CTCTTTGAGA CATTCAAGAT TCCAGTGCGT GAGTTCATGA ACTATTTCCA CGCGCTGGAG 
AATGGATACA GGGACATCCC ATATCACAAC AGAATTCATG CCACAGACGT CCTCCATGCA 
GTCTGGTACC TCACTACACA ACCTGTGCCT GGTTTACCCA CACTGCGGAC ACTCGGCAGC 
TCCGACTCTG ACAGTGGACT CGCTCACGGC CGGATGAAGT ATCTGATGTC CAGGACTAAT 
GCAGTGGAGG AGGAAGGTTA CGGCTGTCTT TCAGGCCTCA TTCCTGCGCT GGAGCTCATG 
GCGCTGTACG TCGCTGCTGC AATGCATGAT TATGACCATC CTGGTCGGAC CAACGCCTTC 
CTGGTGGCCA CCAGCGCTCC ACAGGCTGTG CTATACAATG ACCGTTCAGT GCTGGAGAAT 
CATCACGCTG CCTCCGCCTG GAACCTCTTC ATGTCGCGGC CCGAGTACAA CTTTCTGGCC 
AGTTTGGACC ACATGGAGTT TAAACGCTTT CGCTTCCTGG TCATCGAAGC CATTCTGGCC 
ACTGACCTCA AGAAACACTT TGATTTCCTG GCCGAATTCA ACGCCAAGGT GGGTGATGAC 
GGCTGCTCAG GGATCGACTG GACCAATGAG AACGACCGTC TGCTGGTCTG TCAGATGTGC 
ATTAAGCTAG CAGACATAAA CGGGCCGCTG AAATGCAAGG ATCTTCATTT GCAGTGGACG 
GAGGGCATCG TCAATGAGTT TTACGAGCAG GGTGATGAAG AGTCCAGTCT CGGTCTTCCC 
ATCAGCCCCT TCATGGACCG CTCAGCACCT CAGCTGGCCA AACTGCAAGA GTCCTTCATC 
ACACACATCG TGGGTCCTCT GTGCAGCTCG TATGACTCTG CGGCGCTCAT GCCTGGCCGC 
TGGGTCCACC CTCTGCCCAA AGATGGAGAT GAGGTGGAGG ACGAGAGCGA GGAGGAGGAT 
GAGGACACAG CGGAGGAGGA GGAAGAGGAC GCCTCCACCA GCTCTGAACT CTCCCAAAAA 
CCAGCACCTA AGAAACGAAG GAAAGTCTTC TGCCAGATCT CCCATCACCT GCTGGAGAAC 
CATGAGATGT GGAAGAAGGT CATTGCAGAG GAGTCCCAGA GCCAGACTGA AGGGGAGGAG 
TCAACATGCC TGAGCACCAC CCCTGAGCCA ATCCTGGCCA TCGATGAAGA GGAGGAGGAG 
CCAGGGAGCA AGGAGGAGCC AACGGATGAC CAAGGGGAGG GGTCAACAGA GGAAGAGGAG 
GGACCCGTAC CTGAAGACAC GCATGAAACA CAGGAACCAG ATGGCGAGAC TGTAGAGGAA 
GCAGATGAGA AAAACACAGA GACTGTAGAA GACATTGAGG GAAAACAAGA CGAAGACCAG 
AAAGATCCAG AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE3A ( XM_009299995.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009298270.1 Danio rerio phosphodiesterase 3A, cGMP-inhibited (pde3a), transcript variant X2, mRNA.

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CloneID	ODa45059
Clone ID Related Accession (Same CDS sequence)	XM_009299995.3 , XM_009299995.2
Accession Version	XM_009299995.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299995.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG AATCCGTGCG CAACGGCTAT GTGCGCACTT GTGTCACTCC CGTGGAGCAG 
GACTGCTGGC ACTCGTGCTT GCGAAGCCGT AGAAGCCTCT CGTCGGCCGT GTGCGTCGCC 
TCGCTGTCCG TGCTCCTCGC GCTGCTCTGG GACGCGGAGC GCACACACGC GCACTCGGGC 
TCTCACTTGG TGACGCTCGT GGAGCTCGTG TGGCAGCTGC TCTCGCCCGT CCTCACGCTC 
GTGTGTGCCT TCTTCTGGGT GGGTGTGTAT CTGCTCCGCT GCGGGGTGCT GATCCGCACG 
GCAGTGTTCC TGCTCACCGT GTGCCATCTG GGCGAAGCGG CGGCGCAGTC GCTGCTGGAT 
GCGCAGCAGC TGTACTCATT CACGGCCACC GCGCTGGTGC TGCTCGCCTG CCTGGCCAGC 
GGGGCGCTCA TGGTGGTGCG GCTGGGTCAG GGCATCTCCG TGCTGCTCTT CATCAGCATC 
ATCAGGACCG TGTCGCTTCT GTCGCTGCAG AAAGTGCGCG CCAGCTGGAG ACCGTACCTG 
GCGTACCTGC TCGGGCTGCT CGGGGTCCTG CTGGCGAGGT ATGCGGACAG GCTGCTGCCG 
GAGCAGGGCG CATCACACCG GGAGGGATCG AGGGGAGATA TTCCGGTATT TAAGAGGCGC 
AGGAGGTCCA GCTCCGTAGT CTCGTCAGAC ATGGCACAGC ACGGCCAGTC CAGAAGCAAA 
TCGCACAGGA GGACATCTCT GCCCTGCCTG CAGAGAGAAC AGATGCTCCC ACAGCAAGAA 
TGGGACAACA AAAGAGGCGC CCGAGGATCT CAGCCATCCG GCCTCATGCA ATCTTCGGGC 
ACCAGTGTGA CGGTGGATAT CGCGGTGATG GGCGAGGCTC ACGGACTGAT CTCAGACCTG 
CTGGCTGACC AGTCGCTCGC ACCCAACATC TGCAGCACCC TGCGGGCCGT CAGTAACCTG 
CTGAACACCC AGCTCACCTT CCAGCCCCTG CACCACCGGC CACGCCTGGG CCCCCTGCTG 
GCCTTCAGCG ACGGACACAC GTGCTCCGAC TCGGAGGAGG CGCCTGAGAA AGGAGAGAGG 
CTGGCTATTC CAAAGCGTTT GAGGAGGAGT TTGCATCCAG GGCTTTTGAG ACGGATTTCT 
TCCACGTGGA CCACCACCAC CTCCGCCACA GGCCTGCCAA CCCTCGAACC CGGGCCGGTC 
CGACGAGACC GCAGCGCCAG CATCAAAGCA CCTCACGATG GCCCTTCCAG CAGTTTGGTG 
AACAGTGATT CATGGAACAA CTCCAATATG CTCAGCATTT CCAAGAGCAG GTCCATGTCT 
GCTTCATACG CTGCTTCCTC CAACACCACC AACCACATCT ATAGCAAGAG CCGGAGAGGT 
TGCTCTTCTT CCACCATCTC TCCTCTGGCG TCCCCCTGTC ACTCTCCTCC AGTGCAGAGC 
TCTCCGCTGA CCAGCCCCAC CAACAAGACC CCCTCAGTGG AGCTGCCAGA CCCCGAGCTT 
CCTCCACCAC GCAGCCCACA TAAAGCCTTA ACCCACAGTC AGAGCGCACC CAGCTCCAGC 
GCAGCGCTCT GGCCACCGCC AACGCCACTA TGTAGCAGCT GTGGACGGCC GTACAGTAAA 
CAGAGGCACA GTGATGGATC CACAGAGCCA GCAGAGCGAA CACAACAGTC AGACGAGCCG 
GCGATGATGT CATCAGATTA TGACAGCACG TATGAAACCA ACCACAGTGA CAGCAGCGAC 
TTTGCACAGA CTGACGAGGA CAGAGAGGCT CGCAGGACGC TAACGCTAGC CACGCTTATA 
CCAGCCGAGG AGAAGCCCAT CTTGGCCCCG GAGCCGCTGC TGATGGAGGG ACTGGAGCCG 
CTTCTGTGTC AGATCAACAA CTGGAACTTT CCCATCTTCA GCCTGGTGGA GAAAACCAAC 
GGCAGATGTG GCTGCATCCT CAGTCAGATG TCCTACCGAC TGTTTGTCGA CACGGGACTC 
TTTGAGACAT TCAAGATTCC AGTGCGTGAG TTCATGAACT ATTTCCACGC GCTGGAGAAT 
GGATACAGGG ACATCCCATA TCACAACAGA ATTCATGCCA CAGACGTCCT CCATGCAGTC 
TGGTACCTCA CTACACAACC TGTGCCTGGT TTACCCACAC TGCGGACACT CGGCAGCTCC 
GACTCTGACA GTGGACTCGC TCACGGCCGG ATGAAGTATC TGATGTCCAG GACTAATGCA 
GTGGAGGAGG AAGGTTACGG CTGTCTTTCA GGCCTCATTC CTGCGCTGGA GCTCATGGCG 
CTGTACGTCG CTGCTGCAAT GCATGATTAT GACCATCCTG GTCGGACCAA CGCCTTCCTG 
GTGGCCACCA GCGCTCCACA GGCTGTGCTA TACAATGACC GTTCAGTGCT GGAGAATCAT 
CACGCTGCCT CCGCCTGGAA CCTCTTCATG TCGCGGCCCG AGTACAACTT TCTGGCCAGT 
TTGGACCACA TGGAGTTTAA ACGCTTTCGC TTCCTGGTCA TCGAAGCCAT TCTGGCCACT 
GACCTCAAGA AACACTTTGA TTTCCTGGCC GAATTCAACG CCAAGGTGGG TGATGACGGC 
TGCTCAGGGA TCGACTGGAC CAATGAGAAC GACCGTCTGC TGGTCTGTCA GATGTGCATT 
AAGCTAGCAG ACATAAACGG GCCGCTGAAA TGCAAGGATC TTCATTTGCA GTGGACGGAG 
GGCATCGTCA ATGAGTTTTA CGAGCAGGGT GATGAAGAGT CCAGTCTCGG TCTTCCCATC 
AGCCCCTTCA TGGACCGCTC AGCACCTCAG CTGGCCAAAC TGCAAGAGTC CTTCATCACA 
CACATCGTGG GTCCTCTGTG CAGCTCGTAT GACTCTGCGG CGCTCATGCC TGGCCGCTGG 
GTCCACCCTC TGCCCAAAGA TGGAGATGAG GTGGAGGACG AGAGCGAGGA GGAGGATGAG 
GACACAGCGG AGGAGGAGGA AGAGGACGCC TCCACCAGCT CTGAACTCTC CCAAAAACCA 
GCACCTAAGA AACGAAGGAA AGTCTTCTGC CAGATCTCCC ATCACCTGCT GGAGAACCAT 
GAGATGTGGA AGAAGGTCAT TGCAGAGGAG TCCCAGAGCC AGACTGAAGG GGAGGAGTCA 
ACATGCCTGA GCACCACCCC TGAGCCAATC CTGGCCATCG ATGAAGAGGA GGAGGAGCCA 
GGGAGCAAGG AGGAGCCAAC GGATGACCAA GGGGAGGGGT CAACAGAGGA AGAGGAGGGA 
CCCGTACCTG AAGACACGCA TGAAACACAG GAACCAGATG GCGAGACTGT AGAGGAAGCA 
GATGAGAAAA ACACAGAGAC TGTAGAAGAC ATTGAGGGAA AACAAGACGA AGACCAGAAA 
GATCCAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298270.1
CDS	646..4017
Translation	MALESVRNGYVRTCVTPVEQDCWHSCLRSRRSLSSAVCVASLSVLLALLWDAERTHAHSGSHLVTLVELVWQLLSPVLTLVCAFFWVGVYLLRCGVLIRTAVFLLTVCHLGEAAAQSLLDAQQLYSFTATALVLLACLASGALMVVRLGQGISVLLFISIIRTVSLLSLQKVRASWRPYLAYLLGLLGVLLARYADRLLPEQGASHREGSRGDIPVFKRRRRSSSVVSSDMAQHGQSRSKSHRRTSLPCLQREQMLPQQEWDNKRGARGSQPSGLMQSSGTSVTVDIAVMGEAHGLISDLLADQSLAPNICSTLRAVSNLLNTQLTFQPLHHRPRLGPLLAFSDGHTCSDSEEAPEKGERLAIPKRLRRSLHPGLLRRISSTWTTTTSATGLPTLEPGPVRRDRSASIKAPHDGPSSSLVNSDSWNNSNMLSISKSRSMSASYAASSNTTNHIYSKSRRGCSSSTISPLASPCHSPPVQSSPLTSPTNKTPSVELPDPELPPPRSPHKALTHSQSAPSSSAALWPPPTPLCSSCGRPYSKQRHSDGSTEPAERTQQSDEPAMMSSDYDSTYETNHSDSSDFAQTDEDREARRTLTLATLIPAEEKPILAPEPLLMEGLEPLLCQINNWNFPIFSLVEKTNGRCGCILSQMSYRLFVDTGLFETFKIPVREFMNYFHALENGYRDIPYHNRIHATDVLHAVWYLTTQPVPGLPTLRTLGSSDSDSGLAHGRMKYLMSRTNAVEEEGYGCLSGLIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQAVLYNDRSVLENHHAASAWNLFMSRPEYNFLASLDHMEFKRFRFLVIEAILATDLKKHFDFLAEFNAKVGDDGCSGIDWTNENDRLLVCQMCIKLADINGPLKCKDLHLQWTEGIVNEFYEQGDEESSLGLPISPFMDRSAPQLAKLQESFITHIVGPLCSSYDSAALMPGRWVHPLPKDGDEVEDESEEEDEDTAEEEEEDASTSSELSQKPAPKKRRKVFCQISHHLLENHEMWKKVIAEESQSQTEGEESTCLSTTPEPILAIDEEEEEPGSKEEPTDDQGEGSTEEEEGPVPEDTHETQEPDGETVEEADEKNTETVEDIEGKQDEDQKDPE

Target ORF information:

RefSeq Version	XM_009299995.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphodiesterase 3A, cGMP-inhibited (pde3a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299995.3

ORF Insert Sequence:

ATGGCACTGG AATCCGTGCG CAACGGCTAT GTGCGCACTT GTGTCACTCC CGTGGAGCAG 
GACTGCTGGC ACTCGTGCTT GCGAAGCCGT AGAAGCCTCT CGTCGGCCGT GTGCGTCGCC 
TCGCTGTCCG TGCTCCTCGC GCTGCTCTGG GACGCGGAGC GCACACACGC GCACTCGGGC 
TCTCACTTGG TGACGCTCGT GGAGCTCGTG TGGCAGCTGC TCTCGCCCGT CCTCACGCTC 
GTGTGTGCCT TCTTCTGGGT GGGTGTGTAT CTGCTCCGCT GCGGGGTGCT GATCCGCACG 
GCAGTGTTCC TGCTCACCGT GTGCCATCTG GGCGAAGCGG CGGCGCAGTC GCTGCTGGAT 
GCGCAGCAGC TGTACTCATT CACGGCCACC GCGCTGGTGC TGCTCGCCTG CCTGGCCAGC 
GGGGCGCTCA TGGTGGTGCG GCTGGGTCAG GGCATCTCCG TGCTGCTCTT CATCAGCATC 
ATCAGGACCG TGTCGCTTCT GTCGCTGCAG AAAGTGCGCG CCAGCTGGAG ACCGTACCTG 
GCGTACCTGC TCGGGCTGCT CGGGGTCCTG CTGGCGAGGT ATGCGGACAG GCTGCTGCCG 
GAGCAGGGCG CATCACACCG GGAGGGATCG AGGGGAGATA TTCCGGTATT TAAGAGGCGC 
AGGAGGTCCA GCTCCGTAGT CTCGTCAGAC ATGGCACAGC ACGGCCAGTC CAGAAGCAAA 
TCGCACAGGA GGACATCTCT GCCCTGCCTG CAGAGAGAAC AGATGCTCCC ACAGCAAGAA 
TGGGACAACA AAAGAGGCGC CCGAGGATCT CAGCCATCCG GCCTCATGCA ATCTTCGGGC 
ACCAGTGTGA CGGTGGATAT CGCGGTGATG GGCGAGGCTC ACGGACTGAT CTCAGACCTG 
CTGGCTGACC AGTCGCTCGC ACCCAACATC TGCAGCACCC TGCGGGCCGT CAGTAACCTG 
CTGAACACCC AGCTCACCTT CCAGCCCCTG CACCACCGGC CACGCCTGGG CCCCCTGCTG 
GCCTTCAGCG ACGGACACAC GTGCTCCGAC TCGGAGGAGG CGCCTGAGAA AGGAGAGAGG 
CTGGCTATTC CAAAGCGTTT GAGGAGGAGT TTGCATCCAG GGCTTTTGAG ACGGATTTCT 
TCCACGTGGA CCACCACCAC CTCCGCCACA GGCCTGCCAA CCCTCGAACC CGGGCCGGTC 
CGACGAGACC GCAGCGCCAG CATCAAAGCA CCTCACGATG GCCCTTCCAG CAGTTTGGTG 
AACAGTGATT CATGGAACAA CTCCAATATG CTCAGCATTT CCAAGAGCAG GTCCATGTCT 
GCTTCATACG CTGCTTCCTC CAACACCACC AACCACATCT ATAGCAAGAG CCGGAGAGGT 
TGCTCTTCTT CCACCATCTC TCCTCTGGCG TCCCCCTGTC ACTCTCCTCC AGTGCAGAGC 
TCTCCGCTGA CCAGCCCCAC CAACAAGACC CCCTCAGTGG AGCTGCCAGA CCCCGAGCTT 
CCTCCACCAC GCAGCCCACA TAAAGCCTTA ACCCACAGTC AGAGCGCACC CAGCTCCAGC 
GCAGCGCTCT GGCCACCGCC AACGCCACTA TGTAGCAGCT GTGGACGGCC GTACAGTAAA 
CAGAGGCACA GTGATGGATC CACAGAGCCA GCAGAGCGAA CACAACAGTC AGACGAGCCG 
GCGATGATGT CATCAGATTA TGACAGCACG TATGAAACCA ACCACAGTGA CAGCAGCGAC 
TTTGCACAGA CTGACGAGGA CAGAGAGGCT CGCAGGACGC TAACGCTAGC CACGCTTATA 
CCAGCCGAGG AGAAGCCCAT CTTGGCCCCG GAGCCGCTGC TGATGGAGGG ACTGGAGCCG 
CTTCTGTGTC AGATCAACAA CTGGAACTTT CCCATCTTCA GCCTGGTGGA GAAAACCAAC 
GGCAGATGTG GCTGCATCCT CAGTCAGATG TCCTACCGAC TGTTTGTCGA CACGGGACTC 
TTTGAGACAT TCAAGATTCC AGTGCGTGAG TTCATGAACT ATTTCCACGC GCTGGAGAAT 
GGATACAGGG ACATCCCATA TCACAACAGA ATTCATGCCA CAGACGTCCT CCATGCAGTC 
TGGTACCTCA CTACACAACC TGTGCCTGGT TTACCCACAC TGCGGACACT CGGCAGCTCC 
GACTCTGACA GTGGACTCGC TCACGGCCGG ATGAAGTATC TGATGTCCAG GACTAATGCA 
GTGGAGGAGG AAGGTTACGG CTGTCTTTCA GGCCTCATTC CTGCGCTGGA GCTCATGGCG 
CTGTACGTCG CTGCTGCAAT GCATGATTAT GACCATCCTG GTCGGACCAA CGCCTTCCTG 
GTGGCCACCA GCGCTCCACA GGCTGTGCTA TACAATGACC GTTCAGTGCT GGAGAATCAT 
CACGCTGCCT CCGCCTGGAA CCTCTTCATG TCGCGGCCCG AGTACAACTT TCTGGCCAGT 
TTGGACCACA TGGAGTTTAA ACGCTTTCGC TTCCTGGTCA TCGAAGCCAT TCTGGCCACT 
GACCTCAAGA AACACTTTGA TTTCCTGGCC GAATTCAACG CCAAGGTGGG TGATGACGGC 
TGCTCAGGGA TCGACTGGAC CAATGAGAAC GACCGTCTGC TGGTCTGTCA GATGTGCATT 
AAGCTAGCAG ACATAAACGG GCCGCTGAAA TGCAAGGATC TTCATTTGCA GTGGACGGAG 
GGCATCGTCA ATGAGTTTTA CGAGCAGGGT GATGAAGAGT CCAGTCTCGG TCTTCCCATC 
AGCCCCTTCA TGGACCGCTC AGCACCTCAG CTGGCCAAAC TGCAAGAGTC CTTCATCACA 
CACATCGTGG GTCCTCTGTG CAGCTCGTAT GACTCTGCGG CGCTCATGCC TGGCCGCTGG 
GTCCACCCTC TGCCCAAAGA TGGAGATGAG GTGGAGGACG AGAGCGAGGA GGAGGATGAG 
GACACAGCGG AGGAGGAGGA AGAGGACGCC TCCACCAGCT CTGAACTCTC CCAAAAACCA 
GCACCTAAGA AACGAAGGAA AGTCTTCTGC CAGATCTCCC ATCACCTGCT GGAGAACCAT 
GAGATGTGGA AGAAGGTCAT TGCAGAGGAG TCCCAGAGCC AGACTGAAGG GGAGGAGTCA 
ACATGCCTGA GCACCACCCC TGAGCCAATC CTGGCCATCG ATGAAGAGGA GGAGGAGCCA 
GGGAGCAAGG AGGAGCCAAC GGATGACCAA GGGGAGGGGT CAACAGAGGA AGAGGAGGGA 
CCCGTACCTG AAGACACGCA TGAAACACAG GAACCAGATG GCGAGACTGT AGAGGAAGCA 
GATGAGAAAA ACACAGAGAC TGTAGAAGAC ATTGAGGGAA AACAAGACGA AGACCAGAAA 
GATCCAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE3A ( XM_009299995.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009298270.1 Danio rerio phosphodiesterase 3A, cGMP-inhibited (pde3a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa45059
Clone ID Related Accession (Same CDS sequence)	XM_009299995.3 , XM_009299995.2
Accession Version	XM_009299995.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299995.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG AATCCGTGCG CAACGGCTAT GTGCGCACTT GTGTCACTCC CGTGGAGCAG 
GACTGCTGGC ACTCGTGCTT GCGAAGCCGT AGAAGCCTCT CGTCGGCCGT GTGCGTCGCC 
TCGCTGTCCG TGCTCCTCGC GCTGCTCTGG GACGCGGAGC GCACACACGC GCACTCGGGC 
TCTCACTTGG TGACGCTCGT GGAGCTCGTG TGGCAGCTGC TCTCGCCCGT CCTCACGCTC 
GTGTGTGCCT TCTTCTGGGT GGGTGTGTAT CTGCTCCGCT GCGGGGTGCT GATCCGCACG 
GCAGTGTTCC TGCTCACCGT GTGCCATCTG GGCGAAGCGG CGGCGCAGTC GCTGCTGGAT 
GCGCAGCAGC TGTACTCATT CACGGCCACC GCGCTGGTGC TGCTCGCCTG CCTGGCCAGC 
GGGGCGCTCA TGGTGGTGCG GCTGGGTCAG GGCATCTCCG TGCTGCTCTT CATCAGCATC 
ATCAGGACCG TGTCGCTTCT GTCGCTGCAG AAAGTGCGCG CCAGCTGGAG ACCGTACCTG 
GCGTACCTGC TCGGGCTGCT CGGGGTCCTG CTGGCGAGGT ATGCGGACAG GCTGCTGCCG 
GAGCAGGGCG CATCACACCG GGAGGGATCG AGGGGAGATA TTCCGGTATT TAAGAGGCGC 
AGGAGGTCCA GCTCCGTAGT CTCGTCAGAC ATGGCACAGC ACGGCCAGTC CAGAAGCAAA 
TCGCACAGGA GGACATCTCT GCCCTGCCTG CAGAGAGAAC AGATGCTCCC ACAGCAAGAA 
TGGGACAACA AAAGAGGCGC CCGAGGATCT CAGCCATCCG GCCTCATGCA ATCTTCGGGC 
ACCAGTGTGA CGGTGGATAT CGCGGTGATG GGCGAGGCTC ACGGACTGAT CTCAGACCTG 
CTGGCTGACC AGTCGCTCGC ACCCAACATC TGCAGCACCC TGCGGGCCGT CAGTAACCTG 
CTGAACACCC AGCTCACCTT CCAGCCCCTG CACCACCGGC CACGCCTGGG CCCCCTGCTG 
GCCTTCAGCG ACGGACACAC GTGCTCCGAC TCGGAGGAGG CGCCTGAGAA AGGAGAGAGG 
CTGGCTATTC CAAAGCGTTT GAGGAGGAGT TTGCATCCAG GGCTTTTGAG ACGGATTTCT 
TCCACGTGGA CCACCACCAC CTCCGCCACA GGCCTGCCAA CCCTCGAACC CGGGCCGGTC 
CGACGAGACC GCAGCGCCAG CATCAAAGCA CCTCACGATG GCCCTTCCAG CAGTTTGGTG 
AACAGTGATT CATGGAACAA CTCCAATATG CTCAGCATTT CCAAGAGCAG GTCCATGTCT 
GCTTCATACG CTGCTTCCTC CAACACCACC AACCACATCT ATAGCAAGAG CCGGAGAGGT 
TGCTCTTCTT CCACCATCTC TCCTCTGGCG TCCCCCTGTC ACTCTCCTCC AGTGCAGAGC 
TCTCCGCTGA CCAGCCCCAC CAACAAGACC CCCTCAGTGG AGCTGCCAGA CCCCGAGCTT 
CCTCCACCAC GCAGCCCACA TAAAGCCTTA ACCCACAGTC AGAGCGCACC CAGCTCCAGC 
GCAGCGCTCT GGCCACCGCC AACGCCACTA TGTAGCAGCT GTGGACGGCC GTACAGTAAA 
CAGAGGCACA GTGATGGATC CACAGAGCCA GCAGAGCGAA CACAACAGTC AGACGAGCCG 
GCGATGATGT CATCAGATTA TGACAGCACG TATGAAACCA ACCACAGTGA CAGCAGCGAC 
TTTGCACAGA CTGACGAGGA CAGAGAGGCT CGCAGGACGC TAACGCTAGC CACGCTTATA 
CCAGCCGAGG AGAAGCCCAT CTTGGCCCCG GAGCCGCTGC TGATGGAGGG ACTGGAGCCG 
CTTCTGTGTC AGATCAACAA CTGGAACTTT CCCATCTTCA GCCTGGTGGA GAAAACCAAC 
GGCAGATGTG GCTGCATCCT CAGTCAGATG TCCTACCGAC TGTTTGTCGA CACGGGACTC 
TTTGAGACAT TCAAGATTCC AGTGCGTGAG TTCATGAACT ATTTCCACGC GCTGGAGAAT 
GGATACAGGG ACATCCCATA TCACAACAGA ATTCATGCCA CAGACGTCCT CCATGCAGTC 
TGGTACCTCA CTACACAACC TGTGCCTGGT TTACCCACAC TGCGGACACT CGGCAGCTCC 
GACTCTGACA GTGGACTCGC TCACGGCCGG ATGAAGTATC TGATGTCCAG GACTAATGCA 
GTGGAGGAGG AAGGTTACGG CTGTCTTTCA GGCCTCATTC CTGCGCTGGA GCTCATGGCG 
CTGTACGTCG CTGCTGCAAT GCATGATTAT GACCATCCTG GTCGGACCAA CGCCTTCCTG 
GTGGCCACCA GCGCTCCACA GGCTGTGCTA TACAATGACC GTTCAGTGCT GGAGAATCAT 
CACGCTGCCT CCGCCTGGAA CCTCTTCATG TCGCGGCCCG AGTACAACTT TCTGGCCAGT 
TTGGACCACA TGGAGTTTAA ACGCTTTCGC TTCCTGGTCA TCGAAGCCAT TCTGGCCACT 
GACCTCAAGA AACACTTTGA TTTCCTGGCC GAATTCAACG CCAAGGTGGG TGATGACGGC 
TGCTCAGGGA TCGACTGGAC CAATGAGAAC GACCGTCTGC TGGTCTGTCA GATGTGCATT 
AAGCTAGCAG ACATAAACGG GCCGCTGAAA TGCAAGGATC TTCATTTGCA GTGGACGGAG 
GGCATCGTCA ATGAGTTTTA CGAGCAGGGT GATGAAGAGT CCAGTCTCGG TCTTCCCATC 
AGCCCCTTCA TGGACCGCTC AGCACCTCAG CTGGCCAAAC TGCAAGAGTC CTTCATCACA 
CACATCGTGG GTCCTCTGTG CAGCTCGTAT GACTCTGCGG CGCTCATGCC TGGCCGCTGG 
GTCCACCCTC TGCCCAAAGA TGGAGATGAG GTGGAGGACG AGAGCGAGGA GGAGGATGAG 
GACACAGCGG AGGAGGAGGA AGAGGACGCC TCCACCAGCT CTGAACTCTC CCAAAAACCA 
GCACCTAAGA AACGAAGGAA AGTCTTCTGC CAGATCTCCC ATCACCTGCT GGAGAACCAT 
GAGATGTGGA AGAAGGTCAT TGCAGAGGAG TCCCAGAGCC AGACTGAAGG GGAGGAGTCA 
ACATGCCTGA GCACCACCCC TGAGCCAATC CTGGCCATCG ATGAAGAGGA GGAGGAGCCA 
GGGAGCAAGG AGGAGCCAAC GGATGACCAA GGGGAGGGGT CAACAGAGGA AGAGGAGGGA 
CCCGTACCTG AAGACACGCA TGAAACACAG GAACCAGATG GCGAGACTGT AGAGGAAGCA 
GATGAGAAAA ACACAGAGAC TGTAGAAGAC ATTGAGGGAA AACAAGACGA AGACCAGAAA 
GATCCAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298270.1
CDS	646..4017
Translation	MALESVRNGYVRTCVTPVEQDCWHSCLRSRRSLSSAVCVASLSVLLALLWDAERTHAHSGSHLVTLVELVWQLLSPVLTLVCAFFWVGVYLLRCGVLIRTAVFLLTVCHLGEAAAQSLLDAQQLYSFTATALVLLACLASGALMVVRLGQGISVLLFISIIRTVSLLSLQKVRASWRPYLAYLLGLLGVLLARYADRLLPEQGASHREGSRGDIPVFKRRRRSSSVVSSDMAQHGQSRSKSHRRTSLPCLQREQMLPQQEWDNKRGARGSQPSGLMQSSGTSVTVDIAVMGEAHGLISDLLADQSLAPNICSTLRAVSNLLNTQLTFQPLHHRPRLGPLLAFSDGHTCSDSEEAPEKGERLAIPKRLRRSLHPGLLRRISSTWTTTTSATGLPTLEPGPVRRDRSASIKAPHDGPSSSLVNSDSWNNSNMLSISKSRSMSASYAASSNTTNHIYSKSRRGCSSSTISPLASPCHSPPVQSSPLTSPTNKTPSVELPDPELPPPRSPHKALTHSQSAPSSSAALWPPPTPLCSSCGRPYSKQRHSDGSTEPAERTQQSDEPAMMSSDYDSTYETNHSDSSDFAQTDEDREARRTLTLATLIPAEEKPILAPEPLLMEGLEPLLCQINNWNFPIFSLVEKTNGRCGCILSQMSYRLFVDTGLFETFKIPVREFMNYFHALENGYRDIPYHNRIHATDVLHAVWYLTTQPVPGLPTLRTLGSSDSDSGLAHGRMKYLMSRTNAVEEEGYGCLSGLIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQAVLYNDRSVLENHHAASAWNLFMSRPEYNFLASLDHMEFKRFRFLVIEAILATDLKKHFDFLAEFNAKVGDDGCSGIDWTNENDRLLVCQMCIKLADINGPLKCKDLHLQWTEGIVNEFYEQGDEESSLGLPISPFMDRSAPQLAKLQESFITHIVGPLCSSYDSAALMPGRWVHPLPKDGDEVEDESEEEDEDTAEEEEEDASTSSELSQKPAPKKRRKVFCQISHHLLENHEMWKKVIAEESQSQTEGEESTCLSTTPEPILAIDEEEEEPGSKEEPTDDQGEGSTEEEEGPVPEDTHETQEPDGETVEEADEKNTETVEDIEGKQDEDQKDPE

Target ORF information:

RefSeq Version	XM_009299995.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphodiesterase 3A, cGMP-inhibited (pde3a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299995.2

ORF Insert Sequence:

ATGGCACTGG AATCCGTGCG CAACGGCTAT GTGCGCACTT GTGTCACTCC CGTGGAGCAG 
GACTGCTGGC ACTCGTGCTT GCGAAGCCGT AGAAGCCTCT CGTCGGCCGT GTGCGTCGCC 
TCGCTGTCCG TGCTCCTCGC GCTGCTCTGG GACGCGGAGC GCACACACGC GCACTCGGGC 
TCTCACTTGG TGACGCTCGT GGAGCTCGTG TGGCAGCTGC TCTCGCCCGT CCTCACGCTC 
GTGTGTGCCT TCTTCTGGGT GGGTGTGTAT CTGCTCCGCT GCGGGGTGCT GATCCGCACG 
GCAGTGTTCC TGCTCACCGT GTGCCATCTG GGCGAAGCGG CGGCGCAGTC GCTGCTGGAT 
GCGCAGCAGC TGTACTCATT CACGGCCACC GCGCTGGTGC TGCTCGCCTG CCTGGCCAGC 
GGGGCGCTCA TGGTGGTGCG GCTGGGTCAG GGCATCTCCG TGCTGCTCTT CATCAGCATC 
ATCAGGACCG TGTCGCTTCT GTCGCTGCAG AAAGTGCGCG CCAGCTGGAG ACCGTACCTG 
GCGTACCTGC TCGGGCTGCT CGGGGTCCTG CTGGCGAGGT ATGCGGACAG GCTGCTGCCG 
GAGCAGGGCG CATCACACCG GGAGGGATCG AGGGGAGATA TTCCGGTATT TAAGAGGCGC 
AGGAGGTCCA GCTCCGTAGT CTCGTCAGAC ATGGCACAGC ACGGCCAGTC CAGAAGCAAA 
TCGCACAGGA GGACATCTCT GCCCTGCCTG CAGAGAGAAC AGATGCTCCC ACAGCAAGAA 
TGGGACAACA AAAGAGGCGC CCGAGGATCT CAGCCATCCG GCCTCATGCA ATCTTCGGGC 
ACCAGTGTGA CGGTGGATAT CGCGGTGATG GGCGAGGCTC ACGGACTGAT CTCAGACCTG 
CTGGCTGACC AGTCGCTCGC ACCCAACATC TGCAGCACCC TGCGGGCCGT CAGTAACCTG 
CTGAACACCC AGCTCACCTT CCAGCCCCTG CACCACCGGC CACGCCTGGG CCCCCTGCTG 
GCCTTCAGCG ACGGACACAC GTGCTCCGAC TCGGAGGAGG CGCCTGAGAA AGGAGAGAGG 
CTGGCTATTC CAAAGCGTTT GAGGAGGAGT TTGCATCCAG GGCTTTTGAG ACGGATTTCT 
TCCACGTGGA CCACCACCAC CTCCGCCACA GGCCTGCCAA CCCTCGAACC CGGGCCGGTC 
CGACGAGACC GCAGCGCCAG CATCAAAGCA CCTCACGATG GCCCTTCCAG CAGTTTGGTG 
AACAGTGATT CATGGAACAA CTCCAATATG CTCAGCATTT CCAAGAGCAG GTCCATGTCT 
GCTTCATACG CTGCTTCCTC CAACACCACC AACCACATCT ATAGCAAGAG CCGGAGAGGT 
TGCTCTTCTT CCACCATCTC TCCTCTGGCG TCCCCCTGTC ACTCTCCTCC AGTGCAGAGC 
TCTCCGCTGA CCAGCCCCAC CAACAAGACC CCCTCAGTGG AGCTGCCAGA CCCCGAGCTT 
CCTCCACCAC GCAGCCCACA TAAAGCCTTA ACCCACAGTC AGAGCGCACC CAGCTCCAGC 
GCAGCGCTCT GGCCACCGCC AACGCCACTA TGTAGCAGCT GTGGACGGCC GTACAGTAAA 
CAGAGGCACA GTGATGGATC CACAGAGCCA GCAGAGCGAA CACAACAGTC AGACGAGCCG 
GCGATGATGT CATCAGATTA TGACAGCACG TATGAAACCA ACCACAGTGA CAGCAGCGAC 
TTTGCACAGA CTGACGAGGA CAGAGAGGCT CGCAGGACGC TAACGCTAGC CACGCTTATA 
CCAGCCGAGG AGAAGCCCAT CTTGGCCCCG GAGCCGCTGC TGATGGAGGG ACTGGAGCCG 
CTTCTGTGTC AGATCAACAA CTGGAACTTT CCCATCTTCA GCCTGGTGGA GAAAACCAAC 
GGCAGATGTG GCTGCATCCT CAGTCAGATG TCCTACCGAC TGTTTGTCGA CACGGGACTC 
TTTGAGACAT TCAAGATTCC AGTGCGTGAG TTCATGAACT ATTTCCACGC GCTGGAGAAT 
GGATACAGGG ACATCCCATA TCACAACAGA ATTCATGCCA CAGACGTCCT CCATGCAGTC 
TGGTACCTCA CTACACAACC TGTGCCTGGT TTACCCACAC TGCGGACACT CGGCAGCTCC 
GACTCTGACA GTGGACTCGC TCACGGCCGG ATGAAGTATC TGATGTCCAG GACTAATGCA 
GTGGAGGAGG AAGGTTACGG CTGTCTTTCA GGCCTCATTC CTGCGCTGGA GCTCATGGCG 
CTGTACGTCG CTGCTGCAAT GCATGATTAT GACCATCCTG GTCGGACCAA CGCCTTCCTG 
GTGGCCACCA GCGCTCCACA GGCTGTGCTA TACAATGACC GTTCAGTGCT GGAGAATCAT 
CACGCTGCCT CCGCCTGGAA CCTCTTCATG TCGCGGCCCG AGTACAACTT TCTGGCCAGT 
TTGGACCACA TGGAGTTTAA ACGCTTTCGC TTCCTGGTCA TCGAAGCCAT TCTGGCCACT 
GACCTCAAGA AACACTTTGA TTTCCTGGCC GAATTCAACG CCAAGGTGGG TGATGACGGC 
TGCTCAGGGA TCGACTGGAC CAATGAGAAC GACCGTCTGC TGGTCTGTCA GATGTGCATT 
AAGCTAGCAG ACATAAACGG GCCGCTGAAA TGCAAGGATC TTCATTTGCA GTGGACGGAG 
GGCATCGTCA ATGAGTTTTA CGAGCAGGGT GATGAAGAGT CCAGTCTCGG TCTTCCCATC 
AGCCCCTTCA TGGACCGCTC AGCACCTCAG CTGGCCAAAC TGCAAGAGTC CTTCATCACA 
CACATCGTGG GTCCTCTGTG CAGCTCGTAT GACTCTGCGG CGCTCATGCC TGGCCGCTGG 
GTCCACCCTC TGCCCAAAGA TGGAGATGAG GTGGAGGACG AGAGCGAGGA GGAGGATGAG 
GACACAGCGG AGGAGGAGGA AGAGGACGCC TCCACCAGCT CTGAACTCTC CCAAAAACCA 
GCACCTAAGA AACGAAGGAA AGTCTTCTGC CAGATCTCCC ATCACCTGCT GGAGAACCAT 
GAGATGTGGA AGAAGGTCAT TGCAGAGGAG TCCCAGAGCC AGACTGAAGG GGAGGAGTCA 
ACATGCCTGA GCACCACCCC TGAGCCAATC CTGGCCATCG ATGAAGAGGA GGAGGAGCCA 
GGGAGCAAGG AGGAGCCAAC GGATGACCAA GGGGAGGGGT CAACAGAGGA AGAGGAGGGA 
CCCGTACCTG AAGACACGCA TGAAACACAG GAACCAGATG GCGAGACTGT AGAGGAAGCA 
GATGAGAAAA ACACAGAGAC TGTAGAAGAC ATTGAGGGAA AACAAGACGA AGACCAGAAA 
GATCCAGAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE3A ( XM_009299996.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009298271.1 Danio rerio phosphodiesterase 3A, cGMP-inhibited (pde3a), transcript variant X3, mRNA.

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CloneID	ODa45060
Clone ID Related Accession (Same CDS sequence)	XM_009299996.3 , XM_009299996.2
Accession Version	XM_009299996.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299996.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG AATCCGTGCG CAACGGCTAT GTGCGCACTT GTGTCACTCC CGTGGAGCAG 
GACTGCTGGC ACTCGTGCTT GCGAAGCCGT AGAAGCCTCT CGTCGGCCGT GTGCGTCGCC 
TCGCTGTCCG TGCTCCTCGC GCTGCTCTGG GACGCGGAGC GCACACACGC GCACTCGGGC 
TCTCACTTGG TGACGCTCGT GGAGCTCGTG TGGCAGCTGC TCTCGCCCGT CCTCACGCTC 
GTGTGTGCCT TCTTCTGGGT GGGTGTGTAT CTGCTCCGCT GCGGGGTGCT GATCCGCACG 
GCAGTGTTCC TGCTCACCGT GTGCCATCTG GGCGAAGCGG CGGCGCAGTC GCTGCTGGAT 
GCGCAGCAGC TGTACTCATT CACGGCCACC GCGCTGGTGC TGCTCGCCTG CCTGGCCAGC 
GGGGCGCTCA TGGTGGTGCG GCTGGGTCAG GGCATCTCCG TGCTGCTCTT CATCAGCATC 
ATCAGGACCG TGTCGCTTCT GTCGCTGCAG AAAGTGCGCG CCAGCTGGAG ACCGTACCTG 
GCGTACCTGC TCGGGCTGCT CGGGGTCCTG CTGGCGAGGT ATGCGGACAG GCTGCTGCCG 
GAGCAGGGCG CATCACACCG GGAGGGATCG AGGGGAGATA TTCCGGTATT TAAGAGGCGC 
AGGAGGTCCA GCTCCGTAGT CTCGTCAGAC ATGGCACAGC ACGGCCAGTC CAGAAGCAAA 
TCGCACAGGA GGACATCTCT GCCCTGCCTG CAGAGAGAAC AGATGCTCCC ACAGCAAGAA 
TGGGACAACA AAAGAGGCGC CCGAGGATCT CAGTCTTCGG GCACCAGTGT GACGGTGGAT 
ATCGCGGTGA TGGGCGAGGC TCACGGACTG ATCTCAGACC TGCTGGCTGA CCAGTCGCTC 
GCACCCAACA TCTGCAGCAC CCTGCGGGCC GTCAGTAACC TGCTGAACAC CCAGCTCACC 
TTCCAGCCCC TGCACCACCG GCCACGCCTG GGCCCCCTGC TGGCCTTCAG CGACGGACAC 
ACGTGCTCCG ACTCGGAGGA GGCGCCTGAG AAAGGAGAGA GGCTGGCTAT TCCAAAGCGT 
TTGAGGAGGA GTTTGCATCC AGGGCTTTTG AGACGGATTT CTTCCACGTG GACCACCACC 
ACCTCCGCCA CAGGCCTGCC AACCCTCGAA CCCGGGCCGG TCCGACGAGA CCGCAGCGCC 
AGCATCAAAG CACCTCACGA TGGCCCTTCC AGCAGTTTGG TGAACAGTGA TTCATGGAAC 
AACTCCAATA TGCTCAGCAT TTCCAAGAGC AGGTCCATGT CTGCTTCATA CGCTGCTTCC 
TCCAACACCA CCAACCACAT CTATAGCAAG AGCCGGAGAG GTTGCTCTTC TTCCACCATC 
TCTCCTCTGG CGTCCCCCTG TCACTCTCCT CCAGTGCAGA GCTCTCCGCT GACCAGCCCC 
ACCAACAAGA CCCCCTCAGT GGAGCTGCCA GACCCCGAGC TTCCTCCACC ACGCAGCCCA 
CATAAAGCCT TAACCCACAG TCAGAGCGCA CCCAGCTCCA GCGCAGCGCT CTGGCCACCG 
CCAACGCCAC TATGTAGCAG CTGTGGACGG CCGTACAGTA AACAGAGGCA CAGTGATGGA 
TCCACAGAGC CAGCAGAGCG AACACAACAG TCAGACGAGC CGGCGATGAT GTCATCAGAT 
TATGACAGCA CGTATGAAAC CAACCACAGT GACAGCAGCG ACTTTGCACA GACTGACGAG 
GACAGAGAGG CTCGCAGGAC GCTAACGCTA GCCACGCTTA TACCAGCCGA GGAGAAGCCC 
ATCTTGGCCC CGGAGCCGCT GCTGATGGAG GGACTGGAGC CGCTTCTGTG TCAGATCAAC 
AACTGGAACT TTCCCATCTT CAGCCTGGTG GAGAAAACCA ACGGCAGATG TGGCTGCATC 
CTCAGTCAGA TGTCCTACCG ACTGTTTGTC GACACGGGAC TCTTTGAGAC ATTCAAGATT 
CCAGTGCGTG AGTTCATGAA CTATTTCCAC GCGCTGGAGA ATGGATACAG GGACATCCCA 
TATCACAACA GAATTCATGC CACAGACGTC CTCCATGCAG TCTGGTACCT CACTACACAA 
CCTGTGCCTG GTTTACCCAC ACTGCGGACA CTCGGCAGCT CCGACTCTGA CAGTGGACTC 
GCTCACGGCC GGATGAAGTA TCTGATGTCC AGGACTAATG CAGTGGAGGA GGAAGGTTAC 
GGCTGTCTTT CAGGCCTCAT TCCTGCGCTG GAGCTCATGG CGCTGTACGT CGCTGCTGCA 
ATGCATGATT ATGACCATCC TGGTCGGACC AACGCCTTCC TGGTGGCCAC CAGCGCTCCA 
CAGGCTGTGC TATACAATGA CCGTTCAGTG CTGGAGAATC ATCACGCTGC CTCCGCCTGG 
AACCTCTTCA TGTCGCGGCC CGAGTACAAC TTTCTGGCCA GTTTGGACCA CATGGAGTTT 
AAACGCTTTC GCTTCCTGGT CATCGAAGCC ATTCTGGCCA CTGACCTCAA GAAACACTTT 
GATTTCCTGG CCGAATTCAA CGCCAAGGTG GGTGATGACG GCTGCTCAGG GATCGACTGG 
ACCAATGAGA ACGACCGTCT GCTGGTCTGT CAGATGTGCA TTAAGCTAGC AGACATAAAC 
GGGCCGCTGA AATGCAAGGA TCTTCATTTG CAGTGGACGG AGGGCATCGT CAATGAGTTT 
TACGAGCAGG GTGATGAAGA GTCCAGTCTC GGTCTTCCCA TCAGCCCCTT CATGGACCGC 
TCAGCACCTC AGCTGGCCAA ACTGCAAGAG TCCTTCATCA CACACATCGT GGGTCCTCTG 
TGCAGCTCGT ATGACTCTGC GGCGCTCATG CCTGGCCGCT GGGTCCACCC TCTGCCCAAA 
GATGGAGATG AGGTGGAGGA CGAGAGCGAG GAGGAGGATG AGGACACAGC GGAGGAGGAG 
GAAGAGGACG CCTCCACCAG CTCTGAACTC TCCCAAAAAC CAGCACCTAA GAAACGAAGG 
AAAGTCTTCT GCCAGATCTC CCATCACCTG CTGGAGAACC ATGAGATGTG GAAGAAGGTC 
ATTGCAGAGG AGTCCCAGAG CCAGACTGAA GGGGAGGAGT CAACATGCCT GAGCACCACC 
CCTGAGCCAA TCCTGGCCAT CGATGAAGAG GAGGAGGAGC CAGGGAGCAA GGAGGAGCCA 
ACGGATGACC AAGGGGAGGG GTCAACAGAG GAAGAGGAGG GACCCGTACC TGAAGACACG 
CATGAAACAC AGGAACCAGA TGGCGAGACT GTAGAGGAAG CAGATGAGAA AAACACAGAG 
ACTGTAGAAG ACATTGAGGG AAAACAAGAC GAAGACCAGA AAGATCCAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298271.1
CDS	646..3999
Translation	MALESVRNGYVRTCVTPVEQDCWHSCLRSRRSLSSAVCVASLSVLLALLWDAERTHAHSGSHLVTLVELVWQLLSPVLTLVCAFFWVGVYLLRCGVLIRTAVFLLTVCHLGEAAAQSLLDAQQLYSFTATALVLLACLASGALMVVRLGQGISVLLFISIIRTVSLLSLQKVRASWRPYLAYLLGLLGVLLARYADRLLPEQGASHREGSRGDIPVFKRRRRSSSVVSSDMAQHGQSRSKSHRRTSLPCLQREQMLPQQEWDNKRGARGSQSSGTSVTVDIAVMGEAHGLISDLLADQSLAPNICSTLRAVSNLLNTQLTFQPLHHRPRLGPLLAFSDGHTCSDSEEAPEKGERLAIPKRLRRSLHPGLLRRISSTWTTTTSATGLPTLEPGPVRRDRSASIKAPHDGPSSSLVNSDSWNNSNMLSISKSRSMSASYAASSNTTNHIYSKSRRGCSSSTISPLASPCHSPPVQSSPLTSPTNKTPSVELPDPELPPPRSPHKALTHSQSAPSSSAALWPPPTPLCSSCGRPYSKQRHSDGSTEPAERTQQSDEPAMMSSDYDSTYETNHSDSSDFAQTDEDREARRTLTLATLIPAEEKPILAPEPLLMEGLEPLLCQINNWNFPIFSLVEKTNGRCGCILSQMSYRLFVDTGLFETFKIPVREFMNYFHALENGYRDIPYHNRIHATDVLHAVWYLTTQPVPGLPTLRTLGSSDSDSGLAHGRMKYLMSRTNAVEEEGYGCLSGLIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQAVLYNDRSVLENHHAASAWNLFMSRPEYNFLASLDHMEFKRFRFLVIEAILATDLKKHFDFLAEFNAKVGDDGCSGIDWTNENDRLLVCQMCIKLADINGPLKCKDLHLQWTEGIVNEFYEQGDEESSLGLPISPFMDRSAPQLAKLQESFITHIVGPLCSSYDSAALMPGRWVHPLPKDGDEVEDESEEEDEDTAEEEEEDASTSSELSQKPAPKKRRKVFCQISHHLLENHEMWKKVIAEESQSQTEGEESTCLSTTPEPILAIDEEEEEPGSKEEPTDDQGEGSTEEEEGPVPEDTHETQEPDGETVEEADEKNTETVEDIEGKQDEDQKDPE

Target ORF information:

RefSeq Version	XM_009299996.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphodiesterase 3A, cGMP-inhibited (pde3a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299996.3

ORF Insert Sequence:

ATGGCACTGG AATCCGTGCG CAACGGCTAT GTGCGCACTT GTGTCACTCC CGTGGAGCAG 
GACTGCTGGC ACTCGTGCTT GCGAAGCCGT AGAAGCCTCT CGTCGGCCGT GTGCGTCGCC 
TCGCTGTCCG TGCTCCTCGC GCTGCTCTGG GACGCGGAGC GCACACACGC GCACTCGGGC 
TCTCACTTGG TGACGCTCGT GGAGCTCGTG TGGCAGCTGC TCTCGCCCGT CCTCACGCTC 
GTGTGTGCCT TCTTCTGGGT GGGTGTGTAT CTGCTCCGCT GCGGGGTGCT GATCCGCACG 
GCAGTGTTCC TGCTCACCGT GTGCCATCTG GGCGAAGCGG CGGCGCAGTC GCTGCTGGAT 
GCGCAGCAGC TGTACTCATT CACGGCCACC GCGCTGGTGC TGCTCGCCTG CCTGGCCAGC 
GGGGCGCTCA TGGTGGTGCG GCTGGGTCAG GGCATCTCCG TGCTGCTCTT CATCAGCATC 
ATCAGGACCG TGTCGCTTCT GTCGCTGCAG AAAGTGCGCG CCAGCTGGAG ACCGTACCTG 
GCGTACCTGC TCGGGCTGCT CGGGGTCCTG CTGGCGAGGT ATGCGGACAG GCTGCTGCCG 
GAGCAGGGCG CATCACACCG GGAGGGATCG AGGGGAGATA TTCCGGTATT TAAGAGGCGC 
AGGAGGTCCA GCTCCGTAGT CTCGTCAGAC ATGGCACAGC ACGGCCAGTC CAGAAGCAAA 
TCGCACAGGA GGACATCTCT GCCCTGCCTG CAGAGAGAAC AGATGCTCCC ACAGCAAGAA 
TGGGACAACA AAAGAGGCGC CCGAGGATCT CAGTCTTCGG GCACCAGTGT GACGGTGGAT 
ATCGCGGTGA TGGGCGAGGC TCACGGACTG ATCTCAGACC TGCTGGCTGA CCAGTCGCTC 
GCACCCAACA TCTGCAGCAC CCTGCGGGCC GTCAGTAACC TGCTGAACAC CCAGCTCACC 
TTCCAGCCCC TGCACCACCG GCCACGCCTG GGCCCCCTGC TGGCCTTCAG CGACGGACAC 
ACGTGCTCCG ACTCGGAGGA GGCGCCTGAG AAAGGAGAGA GGCTGGCTAT TCCAAAGCGT 
TTGAGGAGGA GTTTGCATCC AGGGCTTTTG AGACGGATTT CTTCCACGTG GACCACCACC 
ACCTCCGCCA CAGGCCTGCC AACCCTCGAA CCCGGGCCGG TCCGACGAGA CCGCAGCGCC 
AGCATCAAAG CACCTCACGA TGGCCCTTCC AGCAGTTTGG TGAACAGTGA TTCATGGAAC 
AACTCCAATA TGCTCAGCAT TTCCAAGAGC AGGTCCATGT CTGCTTCATA CGCTGCTTCC 
TCCAACACCA CCAACCACAT CTATAGCAAG AGCCGGAGAG GTTGCTCTTC TTCCACCATC 
TCTCCTCTGG CGTCCCCCTG TCACTCTCCT CCAGTGCAGA GCTCTCCGCT GACCAGCCCC 
ACCAACAAGA CCCCCTCAGT GGAGCTGCCA GACCCCGAGC TTCCTCCACC ACGCAGCCCA 
CATAAAGCCT TAACCCACAG TCAGAGCGCA CCCAGCTCCA GCGCAGCGCT CTGGCCACCG 
CCAACGCCAC TATGTAGCAG CTGTGGACGG CCGTACAGTA AACAGAGGCA CAGTGATGGA 
TCCACAGAGC CAGCAGAGCG AACACAACAG TCAGACGAGC CGGCGATGAT GTCATCAGAT 
TATGACAGCA CGTATGAAAC CAACCACAGT GACAGCAGCG ACTTTGCACA GACTGACGAG 
GACAGAGAGG CTCGCAGGAC GCTAACGCTA GCCACGCTTA TACCAGCCGA GGAGAAGCCC 
ATCTTGGCCC CGGAGCCGCT GCTGATGGAG GGACTGGAGC CGCTTCTGTG TCAGATCAAC 
AACTGGAACT TTCCCATCTT CAGCCTGGTG GAGAAAACCA ACGGCAGATG TGGCTGCATC 
CTCAGTCAGA TGTCCTACCG ACTGTTTGTC GACACGGGAC TCTTTGAGAC ATTCAAGATT 
CCAGTGCGTG AGTTCATGAA CTATTTCCAC GCGCTGGAGA ATGGATACAG GGACATCCCA 
TATCACAACA GAATTCATGC CACAGACGTC CTCCATGCAG TCTGGTACCT CACTACACAA 
CCTGTGCCTG GTTTACCCAC ACTGCGGACA CTCGGCAGCT CCGACTCTGA CAGTGGACTC 
GCTCACGGCC GGATGAAGTA TCTGATGTCC AGGACTAATG CAGTGGAGGA GGAAGGTTAC 
GGCTGTCTTT CAGGCCTCAT TCCTGCGCTG GAGCTCATGG CGCTGTACGT CGCTGCTGCA 
ATGCATGATT ATGACCATCC TGGTCGGACC AACGCCTTCC TGGTGGCCAC CAGCGCTCCA 
CAGGCTGTGC TATACAATGA CCGTTCAGTG CTGGAGAATC ATCACGCTGC CTCCGCCTGG 
AACCTCTTCA TGTCGCGGCC CGAGTACAAC TTTCTGGCCA GTTTGGACCA CATGGAGTTT 
AAACGCTTTC GCTTCCTGGT CATCGAAGCC ATTCTGGCCA CTGACCTCAA GAAACACTTT 
GATTTCCTGG CCGAATTCAA CGCCAAGGTG GGTGATGACG GCTGCTCAGG GATCGACTGG 
ACCAATGAGA ACGACCGTCT GCTGGTCTGT CAGATGTGCA TTAAGCTAGC AGACATAAAC 
GGGCCGCTGA AATGCAAGGA TCTTCATTTG CAGTGGACGG AGGGCATCGT CAATGAGTTT 
TACGAGCAGG GTGATGAAGA GTCCAGTCTC GGTCTTCCCA TCAGCCCCTT CATGGACCGC 
TCAGCACCTC AGCTGGCCAA ACTGCAAGAG TCCTTCATCA CACACATCGT GGGTCCTCTG 
TGCAGCTCGT ATGACTCTGC GGCGCTCATG CCTGGCCGCT GGGTCCACCC TCTGCCCAAA 
GATGGAGATG AGGTGGAGGA CGAGAGCGAG GAGGAGGATG AGGACACAGC GGAGGAGGAG 
GAAGAGGACG CCTCCACCAG CTCTGAACTC TCCCAAAAAC CAGCACCTAA GAAACGAAGG 
AAAGTCTTCT GCCAGATCTC CCATCACCTG CTGGAGAACC ATGAGATGTG GAAGAAGGTC 
ATTGCAGAGG AGTCCCAGAG CCAGACTGAA GGGGAGGAGT CAACATGCCT GAGCACCACC 
CCTGAGCCAA TCCTGGCCAT CGATGAAGAG GAGGAGGAGC CAGGGAGCAA GGAGGAGCCA 
ACGGATGACC AAGGGGAGGG GTCAACAGAG GAAGAGGAGG GACCCGTACC TGAAGACACG 
CATGAAACAC AGGAACCAGA TGGCGAGACT GTAGAGGAAG CAGATGAGAA AAACACAGAG 
ACTGTAGAAG ACATTGAGGG AAAACAAGAC GAAGACCAGA AAGATCCAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE3A ( XM_009299996.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009298271.1 Danio rerio phosphodiesterase 3A, cGMP-inhibited (pde3a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa45060
Clone ID Related Accession (Same CDS sequence)	XM_009299996.3 , XM_009299996.2
Accession Version	XM_009299996.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3354bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299996.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG AATCCGTGCG CAACGGCTAT GTGCGCACTT GTGTCACTCC CGTGGAGCAG 
GACTGCTGGC ACTCGTGCTT GCGAAGCCGT AGAAGCCTCT CGTCGGCCGT GTGCGTCGCC 
TCGCTGTCCG TGCTCCTCGC GCTGCTCTGG GACGCGGAGC GCACACACGC GCACTCGGGC 
TCTCACTTGG TGACGCTCGT GGAGCTCGTG TGGCAGCTGC TCTCGCCCGT CCTCACGCTC 
GTGTGTGCCT TCTTCTGGGT GGGTGTGTAT CTGCTCCGCT GCGGGGTGCT GATCCGCACG 
GCAGTGTTCC TGCTCACCGT GTGCCATCTG GGCGAAGCGG CGGCGCAGTC GCTGCTGGAT 
GCGCAGCAGC TGTACTCATT CACGGCCACC GCGCTGGTGC TGCTCGCCTG CCTGGCCAGC 
GGGGCGCTCA TGGTGGTGCG GCTGGGTCAG GGCATCTCCG TGCTGCTCTT CATCAGCATC 
ATCAGGACCG TGTCGCTTCT GTCGCTGCAG AAAGTGCGCG CCAGCTGGAG ACCGTACCTG 
GCGTACCTGC TCGGGCTGCT CGGGGTCCTG CTGGCGAGGT ATGCGGACAG GCTGCTGCCG 
GAGCAGGGCG CATCACACCG GGAGGGATCG AGGGGAGATA TTCCGGTATT TAAGAGGCGC 
AGGAGGTCCA GCTCCGTAGT CTCGTCAGAC ATGGCACAGC ACGGCCAGTC CAGAAGCAAA 
TCGCACAGGA GGACATCTCT GCCCTGCCTG CAGAGAGAAC AGATGCTCCC ACAGCAAGAA 
TGGGACAACA AAAGAGGCGC CCGAGGATCT CAGTCTTCGG GCACCAGTGT GACGGTGGAT 
ATCGCGGTGA TGGGCGAGGC TCACGGACTG ATCTCAGACC TGCTGGCTGA CCAGTCGCTC 
GCACCCAACA TCTGCAGCAC CCTGCGGGCC GTCAGTAACC TGCTGAACAC CCAGCTCACC 
TTCCAGCCCC TGCACCACCG GCCACGCCTG GGCCCCCTGC TGGCCTTCAG CGACGGACAC 
ACGTGCTCCG ACTCGGAGGA GGCGCCTGAG AAAGGAGAGA GGCTGGCTAT TCCAAAGCGT 
TTGAGGAGGA GTTTGCATCC AGGGCTTTTG AGACGGATTT CTTCCACGTG GACCACCACC 
ACCTCCGCCA CAGGCCTGCC AACCCTCGAA CCCGGGCCGG TCCGACGAGA CCGCAGCGCC 
AGCATCAAAG CACCTCACGA TGGCCCTTCC AGCAGTTTGG TGAACAGTGA TTCATGGAAC 
AACTCCAATA TGCTCAGCAT TTCCAAGAGC AGGTCCATGT CTGCTTCATA CGCTGCTTCC 
TCCAACACCA CCAACCACAT CTATAGCAAG AGCCGGAGAG GTTGCTCTTC TTCCACCATC 
TCTCCTCTGG CGTCCCCCTG TCACTCTCCT CCAGTGCAGA GCTCTCCGCT GACCAGCCCC 
ACCAACAAGA CCCCCTCAGT GGAGCTGCCA GACCCCGAGC TTCCTCCACC ACGCAGCCCA 
CATAAAGCCT TAACCCACAG TCAGAGCGCA CCCAGCTCCA GCGCAGCGCT CTGGCCACCG 
CCAACGCCAC TATGTAGCAG CTGTGGACGG CCGTACAGTA AACAGAGGCA CAGTGATGGA 
TCCACAGAGC CAGCAGAGCG AACACAACAG TCAGACGAGC CGGCGATGAT GTCATCAGAT 
TATGACAGCA CGTATGAAAC CAACCACAGT GACAGCAGCG ACTTTGCACA GACTGACGAG 
GACAGAGAGG CTCGCAGGAC GCTAACGCTA GCCACGCTTA TACCAGCCGA GGAGAAGCCC 
ATCTTGGCCC CGGAGCCGCT GCTGATGGAG GGACTGGAGC CGCTTCTGTG TCAGATCAAC 
AACTGGAACT TTCCCATCTT CAGCCTGGTG GAGAAAACCA ACGGCAGATG TGGCTGCATC 
CTCAGTCAGA TGTCCTACCG ACTGTTTGTC GACACGGGAC TCTTTGAGAC ATTCAAGATT 
CCAGTGCGTG AGTTCATGAA CTATTTCCAC GCGCTGGAGA ATGGATACAG GGACATCCCA 
TATCACAACA GAATTCATGC CACAGACGTC CTCCATGCAG TCTGGTACCT CACTACACAA 
CCTGTGCCTG GTTTACCCAC ACTGCGGACA CTCGGCAGCT CCGACTCTGA CAGTGGACTC 
GCTCACGGCC GGATGAAGTA TCTGATGTCC AGGACTAATG CAGTGGAGGA GGAAGGTTAC 
GGCTGTCTTT CAGGCCTCAT TCCTGCGCTG GAGCTCATGG CGCTGTACGT CGCTGCTGCA 
ATGCATGATT ATGACCATCC TGGTCGGACC AACGCCTTCC TGGTGGCCAC CAGCGCTCCA 
CAGGCTGTGC TATACAATGA CCGTTCAGTG CTGGAGAATC ATCACGCTGC CTCCGCCTGG 
AACCTCTTCA TGTCGCGGCC CGAGTACAAC TTTCTGGCCA GTTTGGACCA CATGGAGTTT 
AAACGCTTTC GCTTCCTGGT CATCGAAGCC ATTCTGGCCA CTGACCTCAA GAAACACTTT 
GATTTCCTGG CCGAATTCAA CGCCAAGGTG GGTGATGACG GCTGCTCAGG GATCGACTGG 
ACCAATGAGA ACGACCGTCT GCTGGTCTGT CAGATGTGCA TTAAGCTAGC AGACATAAAC 
GGGCCGCTGA AATGCAAGGA TCTTCATTTG CAGTGGACGG AGGGCATCGT CAATGAGTTT 
TACGAGCAGG GTGATGAAGA GTCCAGTCTC GGTCTTCCCA TCAGCCCCTT CATGGACCGC 
TCAGCACCTC AGCTGGCCAA ACTGCAAGAG TCCTTCATCA CACACATCGT GGGTCCTCTG 
TGCAGCTCGT ATGACTCTGC GGCGCTCATG CCTGGCCGCT GGGTCCACCC TCTGCCCAAA 
GATGGAGATG AGGTGGAGGA CGAGAGCGAG GAGGAGGATG AGGACACAGC GGAGGAGGAG 
GAAGAGGACG CCTCCACCAG CTCTGAACTC TCCCAAAAAC CAGCACCTAA GAAACGAAGG 
AAAGTCTTCT GCCAGATCTC CCATCACCTG CTGGAGAACC ATGAGATGTG GAAGAAGGTC 
ATTGCAGAGG AGTCCCAGAG CCAGACTGAA GGGGAGGAGT CAACATGCCT GAGCACCACC 
CCTGAGCCAA TCCTGGCCAT CGATGAAGAG GAGGAGGAGC CAGGGAGCAA GGAGGAGCCA 
ACGGATGACC AAGGGGAGGG GTCAACAGAG GAAGAGGAGG GACCCGTACC TGAAGACACG 
CATGAAACAC AGGAACCAGA TGGCGAGACT GTAGAGGAAG CAGATGAGAA AAACACAGAG 
ACTGTAGAAG ACATTGAGGG AAAACAAGAC GAAGACCAGA AAGATCCAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298271.1
CDS	646..3999
Translation	MALESVRNGYVRTCVTPVEQDCWHSCLRSRRSLSSAVCVASLSVLLALLWDAERTHAHSGSHLVTLVELVWQLLSPVLTLVCAFFWVGVYLLRCGVLIRTAVFLLTVCHLGEAAAQSLLDAQQLYSFTATALVLLACLASGALMVVRLGQGISVLLFISIIRTVSLLSLQKVRASWRPYLAYLLGLLGVLLARYADRLLPEQGASHREGSRGDIPVFKRRRRSSSVVSSDMAQHGQSRSKSHRRTSLPCLQREQMLPQQEWDNKRGARGSQSSGTSVTVDIAVMGEAHGLISDLLADQSLAPNICSTLRAVSNLLNTQLTFQPLHHRPRLGPLLAFSDGHTCSDSEEAPEKGERLAIPKRLRRSLHPGLLRRISSTWTTTTSATGLPTLEPGPVRRDRSASIKAPHDGPSSSLVNSDSWNNSNMLSISKSRSMSASYAASSNTTNHIYSKSRRGCSSSTISPLASPCHSPPVQSSPLTSPTNKTPSVELPDPELPPPRSPHKALTHSQSAPSSSAALWPPPTPLCSSCGRPYSKQRHSDGSTEPAERTQQSDEPAMMSSDYDSTYETNHSDSSDFAQTDEDREARRTLTLATLIPAEEKPILAPEPLLMEGLEPLLCQINNWNFPIFSLVEKTNGRCGCILSQMSYRLFVDTGLFETFKIPVREFMNYFHALENGYRDIPYHNRIHATDVLHAVWYLTTQPVPGLPTLRTLGSSDSDSGLAHGRMKYLMSRTNAVEEEGYGCLSGLIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQAVLYNDRSVLENHHAASAWNLFMSRPEYNFLASLDHMEFKRFRFLVIEAILATDLKKHFDFLAEFNAKVGDDGCSGIDWTNENDRLLVCQMCIKLADINGPLKCKDLHLQWTEGIVNEFYEQGDEESSLGLPISPFMDRSAPQLAKLQESFITHIVGPLCSSYDSAALMPGRWVHPLPKDGDEVEDESEEEDEDTAEEEEEDASTSSELSQKPAPKKRRKVFCQISHHLLENHEMWKKVIAEESQSQTEGEESTCLSTTPEPILAIDEEEEEPGSKEEPTDDQGEGSTEEEEGPVPEDTHETQEPDGETVEEADEKNTETVEDIEGKQDEDQKDPE

Target ORF information:

RefSeq Version	XM_009299996.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphodiesterase 3A, cGMP-inhibited (pde3a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299996.2

ORF Insert Sequence:

ATGGCACTGG AATCCGTGCG CAACGGCTAT GTGCGCACTT GTGTCACTCC CGTGGAGCAG 
GACTGCTGGC ACTCGTGCTT GCGAAGCCGT AGAAGCCTCT CGTCGGCCGT GTGCGTCGCC 
TCGCTGTCCG TGCTCCTCGC GCTGCTCTGG GACGCGGAGC GCACACACGC GCACTCGGGC 
TCTCACTTGG TGACGCTCGT GGAGCTCGTG TGGCAGCTGC TCTCGCCCGT CCTCACGCTC 
GTGTGTGCCT TCTTCTGGGT GGGTGTGTAT CTGCTCCGCT GCGGGGTGCT GATCCGCACG 
GCAGTGTTCC TGCTCACCGT GTGCCATCTG GGCGAAGCGG CGGCGCAGTC GCTGCTGGAT 
GCGCAGCAGC TGTACTCATT CACGGCCACC GCGCTGGTGC TGCTCGCCTG CCTGGCCAGC 
GGGGCGCTCA TGGTGGTGCG GCTGGGTCAG GGCATCTCCG TGCTGCTCTT CATCAGCATC 
ATCAGGACCG TGTCGCTTCT GTCGCTGCAG AAAGTGCGCG CCAGCTGGAG ACCGTACCTG 
GCGTACCTGC TCGGGCTGCT CGGGGTCCTG CTGGCGAGGT ATGCGGACAG GCTGCTGCCG 
GAGCAGGGCG CATCACACCG GGAGGGATCG AGGGGAGATA TTCCGGTATT TAAGAGGCGC 
AGGAGGTCCA GCTCCGTAGT CTCGTCAGAC ATGGCACAGC ACGGCCAGTC CAGAAGCAAA 
TCGCACAGGA GGACATCTCT GCCCTGCCTG CAGAGAGAAC AGATGCTCCC ACAGCAAGAA 
TGGGACAACA AAAGAGGCGC CCGAGGATCT CAGTCTTCGG GCACCAGTGT GACGGTGGAT 
ATCGCGGTGA TGGGCGAGGC TCACGGACTG ATCTCAGACC TGCTGGCTGA CCAGTCGCTC 
GCACCCAACA TCTGCAGCAC CCTGCGGGCC GTCAGTAACC TGCTGAACAC CCAGCTCACC 
TTCCAGCCCC TGCACCACCG GCCACGCCTG GGCCCCCTGC TGGCCTTCAG CGACGGACAC 
ACGTGCTCCG ACTCGGAGGA GGCGCCTGAG AAAGGAGAGA GGCTGGCTAT TCCAAAGCGT 
TTGAGGAGGA GTTTGCATCC AGGGCTTTTG AGACGGATTT CTTCCACGTG GACCACCACC 
ACCTCCGCCA CAGGCCTGCC AACCCTCGAA CCCGGGCCGG TCCGACGAGA CCGCAGCGCC 
AGCATCAAAG CACCTCACGA TGGCCCTTCC AGCAGTTTGG TGAACAGTGA TTCATGGAAC 
AACTCCAATA TGCTCAGCAT TTCCAAGAGC AGGTCCATGT CTGCTTCATA CGCTGCTTCC 
TCCAACACCA CCAACCACAT CTATAGCAAG AGCCGGAGAG GTTGCTCTTC TTCCACCATC 
TCTCCTCTGG CGTCCCCCTG TCACTCTCCT CCAGTGCAGA GCTCTCCGCT GACCAGCCCC 
ACCAACAAGA CCCCCTCAGT GGAGCTGCCA GACCCCGAGC TTCCTCCACC ACGCAGCCCA 
CATAAAGCCT TAACCCACAG TCAGAGCGCA CCCAGCTCCA GCGCAGCGCT CTGGCCACCG 
CCAACGCCAC TATGTAGCAG CTGTGGACGG CCGTACAGTA AACAGAGGCA CAGTGATGGA 
TCCACAGAGC CAGCAGAGCG AACACAACAG TCAGACGAGC CGGCGATGAT GTCATCAGAT 
TATGACAGCA CGTATGAAAC CAACCACAGT GACAGCAGCG ACTTTGCACA GACTGACGAG 
GACAGAGAGG CTCGCAGGAC GCTAACGCTA GCCACGCTTA TACCAGCCGA GGAGAAGCCC 
ATCTTGGCCC CGGAGCCGCT GCTGATGGAG GGACTGGAGC CGCTTCTGTG TCAGATCAAC 
AACTGGAACT TTCCCATCTT CAGCCTGGTG GAGAAAACCA ACGGCAGATG TGGCTGCATC 
CTCAGTCAGA TGTCCTACCG ACTGTTTGTC GACACGGGAC TCTTTGAGAC ATTCAAGATT 
CCAGTGCGTG AGTTCATGAA CTATTTCCAC GCGCTGGAGA ATGGATACAG GGACATCCCA 
TATCACAACA GAATTCATGC CACAGACGTC CTCCATGCAG TCTGGTACCT CACTACACAA 
CCTGTGCCTG GTTTACCCAC ACTGCGGACA CTCGGCAGCT CCGACTCTGA CAGTGGACTC 
GCTCACGGCC GGATGAAGTA TCTGATGTCC AGGACTAATG CAGTGGAGGA GGAAGGTTAC 
GGCTGTCTTT CAGGCCTCAT TCCTGCGCTG GAGCTCATGG CGCTGTACGT CGCTGCTGCA 
ATGCATGATT ATGACCATCC TGGTCGGACC AACGCCTTCC TGGTGGCCAC CAGCGCTCCA 
CAGGCTGTGC TATACAATGA CCGTTCAGTG CTGGAGAATC ATCACGCTGC CTCCGCCTGG 
AACCTCTTCA TGTCGCGGCC CGAGTACAAC TTTCTGGCCA GTTTGGACCA CATGGAGTTT 
AAACGCTTTC GCTTCCTGGT CATCGAAGCC ATTCTGGCCA CTGACCTCAA GAAACACTTT 
GATTTCCTGG CCGAATTCAA CGCCAAGGTG GGTGATGACG GCTGCTCAGG GATCGACTGG 
ACCAATGAGA ACGACCGTCT GCTGGTCTGT CAGATGTGCA TTAAGCTAGC AGACATAAAC 
GGGCCGCTGA AATGCAAGGA TCTTCATTTG CAGTGGACGG AGGGCATCGT CAATGAGTTT 
TACGAGCAGG GTGATGAAGA GTCCAGTCTC GGTCTTCCCA TCAGCCCCTT CATGGACCGC 
TCAGCACCTC AGCTGGCCAA ACTGCAAGAG TCCTTCATCA CACACATCGT GGGTCCTCTG 
TGCAGCTCGT ATGACTCTGC GGCGCTCATG CCTGGCCGCT GGGTCCACCC TCTGCCCAAA 
GATGGAGATG AGGTGGAGGA CGAGAGCGAG GAGGAGGATG AGGACACAGC GGAGGAGGAG 
GAAGAGGACG CCTCCACCAG CTCTGAACTC TCCCAAAAAC CAGCACCTAA GAAACGAAGG 
AAAGTCTTCT GCCAGATCTC CCATCACCTG CTGGAGAACC ATGAGATGTG GAAGAAGGTC 
ATTGCAGAGG AGTCCCAGAG CCAGACTGAA GGGGAGGAGT CAACATGCCT GAGCACCACC 
CCTGAGCCAA TCCTGGCCAT CGATGAAGAG GAGGAGGAGC CAGGGAGCAA GGAGGAGCCA 
ACGGATGACC AAGGGGAGGG GTCAACAGAG GAAGAGGAGG GACCCGTACC TGAAGACACG 
CATGAAACAC AGGAACCAGA TGGCGAGACT GTAGAGGAAG CAGATGAGAA AAACACAGAG 
ACTGTAGAAG ACATTGAGGG AAAACAAGAC GAAGACCAGA AAGATCCAGA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE3A ( XM_009299997.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009298272.1 Danio rerio phosphodiesterase 3A, cGMP-inhibited (pde3a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa45061
Clone ID Related Accession (Same CDS sequence)	XM_009299997.2 , XM_009299997.3
Accession Version	XM_009299997.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009299997.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG AATCCGTGCG CAACGGCTAT GTGCGCACTT GTGTCACTCC CGTGGAGCAG 
GACTGCTGGC ACTCGTGCTT GCGAAGCCGT AGAAGCCTCT CGTCGGCCGT GTGCGTCGCC 
TCGCTGTCCG TGCTCCTCGC GCTGCTCTGG GACGCGGAGC GCACACACGC GCACTCGGGC 
TCTCACTTGG TGACGCTCGT GGAGCTCGTG TGGCAGCTGC TCTCGCCCGT CCTCACGCTC 
GTGTGTGCCT TCTTCTGGGT GGGTGTGTAT CTGCTCCGCT GCGGGGTGCT GATCCGCACG 
GCAGTGTTCC TGCTCACCGT GTGCCATCTG GGCGAAGCGG CGGCGCAGTC GCTGCTGGAT 
GCGCAGCAGC TGTACTCATT CACGGCCACC GCGCTGGTGC TGCTCGCCTG CCTGGCCAGC 
GGGGCGCTCA TGGTGGTGCG GCTGGGTCAG GGCATCTCCG TGCTGCTCTT CATCAGCATC 
ATCAGGACCG TGTCGCTTCT GTCGCTGCAG AAAGTGCGCG CCAGCTGGAG ACCGTACCTG 
GCGTACCTGC TCGGGCTGCT CGGGGTCCTG CTGGCGAGGT ATGCGGACAG GCTGCTGCCG 
GAGCAGGGCG CATCACACCG GGAGGGATCG AGGGGAGATA TTCCGGTATT TAAGAGGCGC 
AGGAGGTCCA GCTCCGTAGT CTCGTCAGAC ATGGCACAGC ACGGCCAGTC CAGAAGCAAA 
TCGCACAGGA GGACATCTCT GCCCTGCCTG CAGAGAGAAC AGATGCTCCC ACAGCAAGAA 
TGGGACAACA AAAGAGGCGC CCGAGGATCT CAGCAGCCAT CCGGCCTCAT GCAATCTTCG 
GGCACCAGTG TGACGGTGGA TATCGCGGTG ATGGGCGAGG CTCACGGACT GATCTCAGAC 
CTGCTGGCTG ACCAGTCGCT CGCACCCAAC ATCTGCAGCA CCCTGCGGGC CGTCAGTAAC 
CTGCTGAACA CCCAGCTCAC CTTCCAGCCC CTGCACCACC GGCCACGCCT GGGCCCCCTG 
CTGGCCTTCA GCGACGGACA CACGTGCTCC GACTCGGAGG AGGCGCCTGA GAAAGGAGAG 
AGGCTGGCTA TTCCAAAGCG TTTGAGGAGG AGTTTGCATC CAGGGCTTTT GAGACGGATT 
TCTTCCACGT GGACCACCAC CACCTCCGCC ACAGGCCTGC CAACCCTCGA ACCCGGGCCG 
GTCCGACGAG ACCGCAGCGC CAGCATCAAA GCACCTCACG ATGGCCCTTC CAGCAGTTTG 
GTGAACAGTG ATTCATGGAA CAACTCCAAT ATGCTCAGCA TTTCCAAGAG CAGGTCCATG 
TCTGCTTCAT ACGCTGCTTC CTCCAACACC ACCAACCACA TCTATAGCAA GAGCCGGAGA 
GTGCAGAGCT CTCCGCTGAC CAGCCCCACC AACAAGACCC CCTCAGTGGA GCTGCCAGAC 
CCCGAGCTTC CTCCACCACG CAGCCCACAT AAAGCCTTAA CCCACAGTCA GAGCGCACCC 
AGCTCCAGCG CAGCGCTCTG GCCACCGCCA ACGCCACTAT GTAGCAGCTG TGGACGGCCG 
TACAGTAAAC AGAGGCACAG TGATGGATCC ACAGAGCCAG CAGAGCGAAC ACAACAGTCA 
GACGAGCCGG CGATGATGTC ATCAGATTAT GACAGCACGT ATGAAACCAA CCACAGTGAC 
AGCAGCGACT TTGCACAGAC TGACGAGGAC AGAGAGGCTC GCAGGACGCT AACGCTAGCC 
ACGCTTATAC CAGCCGAGGA GAAGCCCATC TTGGCCCCGG AGCCGCTGCT GATGGAGGGA 
CTGGAGCCGC TTCTGTGTCA GATCAACAAC TGGAACTTTC CCATCTTCAG CCTGGTGGAG 
AAAACCAACG GCAGATGTGG CTGCATCCTC AGTCAGATGT CCTACCGACT GTTTGTCGAC 
ACGGGACTCT TTGAGACATT CAAGATTCCA GTGCGTGAGT TCATGAACTA TTTCCACGCG 
CTGGAGAATG GATACAGGGA CATCCCATAT CACAACAGAA TTCATGCCAC AGACGTCCTC 
CATGCAGTCT GGTACCTCAC TACACAACCT GTGCCTGGTT TACCCACACT GCGGACACTC 
GGCAGCTCCG ACTCTGACAG TGGACTCGCT CACGGCCGGA TGAAGTATCT GATGTCCAGG 
ACTAATGCAG TGGAGGAGGA AGGTTACGGC TGTCTTTCAG GCCTCATTCC TGCGCTGGAG 
CTCATGGCGC TGTACGTCGC TGCTGCAATG CATGATTATG ACCATCCTGG TCGGACCAAC 
GCCTTCCTGG TGGCCACCAG CGCTCCACAG GCTGTGCTAT ACAATGACCG TTCAGTGCTG 
GAGAATCATC ACGCTGCCTC CGCCTGGAAC CTCTTCATGT CGCGGCCCGA GTACAACTTT 
CTGGCCAGTT TGGACCACAT GGAGTTTAAA CGCTTTCGCT TCCTGGTCAT CGAAGCCATT 
CTGGCCACTG ACCTCAAGAA ACACTTTGAT TTCCTGGCCG AATTCAACGC CAAGGTGGGT 
GATGACGGCT GCTCAGGGAT CGACTGGACC AATGAGAACG ACCGTCTGCT GGTCTGTCAG 
ATGTGCATTA AGCTAGCAGA CATAAACGGG CCGCTGAAAT GCAAGGATCT TCATTTGCAG 
TGGACGGAGG GCATCGTCAA TGAGTTTTAC GAGCAGGGTG ATGAAGAGTC CAGTCTCGGT 
CTTCCCATCA GCCCCTTCAT GGACCGCTCA GCACCTCAGC TGGCCAAACT GCAAGAGTCC 
TTCATCACAC ACATCGTGGG TCCTCTGTGC AGCTCGTATG ACTCTGCGGC GCTCATGCCT 
GGCCGCTGGG TCCACCCTCT GCCCAAAGAT GGAGATGAGG TGGAGGACGA GAGCGAGGAG 
GAGGATGAGG ACACAGCGGA GGAGGAGGAA GAGGACGCCT CCACCAGCTC TGAACTCTCC 
CAAAAACCAG CACCTAAGAA ACGAAGGAAA GTCTTCTGCC AGATCTCCCA TCACCTGCTG 
GAGAACCATG AGATGTGGAA GAAGGTCATT GCAGAGGAGT CCCAGAGCCA GACTGAAGGG 
GAGGAGTCAA CATGCCTGAG CACCACCCCT GAGCCAATCC TGGCCATCGA TGAAGAGGAG 
GAGGAGCCAG GGAGCAAGGA GGAGCCAACG GATGACCAAG GGGAGGGGTC AACAGAGGAA 
GAGGAGGGAC CCGTACCTGA AGACACGCAT GAAACACAGG AACCAGATGG CGAGACTGTA 
GAGGAAGCAG ATGAGAAAAA CACAGAGACT GTAGAAGACA TTGAGGGAAA ACAAGACGAA 
GACCAGAAAG ATCCAGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298272.1
CDS	646..3966
Translation	MALESVRNGYVRTCVTPVEQDCWHSCLRSRRSLSSAVCVASLSVLLALLWDAERTHAHSGSHLVTLVELVWQLLSPVLTLVCAFFWVGVYLLRCGVLIRTAVFLLTVCHLGEAAAQSLLDAQQLYSFTATALVLLACLASGALMVVRLGQGISVLLFISIIRTVSLLSLQKVRASWRPYLAYLLGLLGVLLARYADRLLPEQGASHREGSRGDIPVFKRRRRSSSVVSSDMAQHGQSRSKSHRRTSLPCLQREQMLPQQEWDNKRGARGSQQPSGLMQSSGTSVTVDIAVMGEAHGLISDLLADQSLAPNICSTLRAVSNLLNTQLTFQPLHHRPRLGPLLAFSDGHTCSDSEEAPEKGERLAIPKRLRRSLHPGLLRRISSTWTTTTSATGLPTLEPGPVRRDRSASIKAPHDGPSSSLVNSDSWNNSNMLSISKSRSMSASYAASSNTTNHIYSKSRRVQSSPLTSPTNKTPSVELPDPELPPPRSPHKALTHSQSAPSSSAALWPPPTPLCSSCGRPYSKQRHSDGSTEPAERTQQSDEPAMMSSDYDSTYETNHSDSSDFAQTDEDREARRTLTLATLIPAEEKPILAPEPLLMEGLEPLLCQINNWNFPIFSLVEKTNGRCGCILSQMSYRLFVDTGLFETFKIPVREFMNYFHALENGYRDIPYHNRIHATDVLHAVWYLTTQPVPGLPTLRTLGSSDSDSGLAHGRMKYLMSRTNAVEEEGYGCLSGLIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQAVLYNDRSVLENHHAASAWNLFMSRPEYNFLASLDHMEFKRFRFLVIEAILATDLKKHFDFLAEFNAKVGDDGCSGIDWTNENDRLLVCQMCIKLADINGPLKCKDLHLQWTEGIVNEFYEQGDEESSLGLPISPFMDRSAPQLAKLQESFITHIVGPLCSSYDSAALMPGRWVHPLPKDGDEVEDESEEEDEDTAEEEEEDASTSSELSQKPAPKKRRKVFCQISHHLLENHEMWKKVIAEESQSQTEGEESTCLSTTPEPILAIDEEEEEPGSKEEPTDDQGEGSTEEEEGPVPEDTHETQEPDGETVEEADEKNTETVEDIEGKQDEDQKDPE

Target ORF information:

RefSeq Version	XM_009299997.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphodiesterase 3A, cGMP-inhibited (pde3a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299997.2

ORF Insert Sequence:

ATGGCACTGG AATCCGTGCG CAACGGCTAT GTGCGCACTT GTGTCACTCC CGTGGAGCAG 
GACTGCTGGC ACTCGTGCTT GCGAAGCCGT AGAAGCCTCT CGTCGGCCGT GTGCGTCGCC 
TCGCTGTCCG TGCTCCTCGC GCTGCTCTGG GACGCGGAGC GCACACACGC GCACTCGGGC 
TCTCACTTGG TGACGCTCGT GGAGCTCGTG TGGCAGCTGC TCTCGCCCGT CCTCACGCTC 
GTGTGTGCCT TCTTCTGGGT GGGTGTGTAT CTGCTCCGCT GCGGGGTGCT GATCCGCACG 
GCAGTGTTCC TGCTCACCGT GTGCCATCTG GGCGAAGCGG CGGCGCAGTC GCTGCTGGAT 
GCGCAGCAGC TGTACTCATT CACGGCCACC GCGCTGGTGC TGCTCGCCTG CCTGGCCAGC 
GGGGCGCTCA TGGTGGTGCG GCTGGGTCAG GGCATCTCCG TGCTGCTCTT CATCAGCATC 
ATCAGGACCG TGTCGCTTCT GTCGCTGCAG AAAGTGCGCG CCAGCTGGAG ACCGTACCTG 
GCGTACCTGC TCGGGCTGCT CGGGGTCCTG CTGGCGAGGT ATGCGGACAG GCTGCTGCCG 
GAGCAGGGCG CATCACACCG GGAGGGATCG AGGGGAGATA TTCCGGTATT TAAGAGGCGC 
AGGAGGTCCA GCTCCGTAGT CTCGTCAGAC ATGGCACAGC ACGGCCAGTC CAGAAGCAAA 
TCGCACAGGA GGACATCTCT GCCCTGCCTG CAGAGAGAAC AGATGCTCCC ACAGCAAGAA 
TGGGACAACA AAAGAGGCGC CCGAGGATCT CAGCAGCCAT CCGGCCTCAT GCAATCTTCG 
GGCACCAGTG TGACGGTGGA TATCGCGGTG ATGGGCGAGG CTCACGGACT GATCTCAGAC 
CTGCTGGCTG ACCAGTCGCT CGCACCCAAC ATCTGCAGCA CCCTGCGGGC CGTCAGTAAC 
CTGCTGAACA CCCAGCTCAC CTTCCAGCCC CTGCACCACC GGCCACGCCT GGGCCCCCTG 
CTGGCCTTCA GCGACGGACA CACGTGCTCC GACTCGGAGG AGGCGCCTGA GAAAGGAGAG 
AGGCTGGCTA TTCCAAAGCG TTTGAGGAGG AGTTTGCATC CAGGGCTTTT GAGACGGATT 
TCTTCCACGT GGACCACCAC CACCTCCGCC ACAGGCCTGC CAACCCTCGA ACCCGGGCCG 
GTCCGACGAG ACCGCAGCGC CAGCATCAAA GCACCTCACG ATGGCCCTTC CAGCAGTTTG 
GTGAACAGTG ATTCATGGAA CAACTCCAAT ATGCTCAGCA TTTCCAAGAG CAGGTCCATG 
TCTGCTTCAT ACGCTGCTTC CTCCAACACC ACCAACCACA TCTATAGCAA GAGCCGGAGA 
GTGCAGAGCT CTCCGCTGAC CAGCCCCACC AACAAGACCC CCTCAGTGGA GCTGCCAGAC 
CCCGAGCTTC CTCCACCACG CAGCCCACAT AAAGCCTTAA CCCACAGTCA GAGCGCACCC 
AGCTCCAGCG CAGCGCTCTG GCCACCGCCA ACGCCACTAT GTAGCAGCTG TGGACGGCCG 
TACAGTAAAC AGAGGCACAG TGATGGATCC ACAGAGCCAG CAGAGCGAAC ACAACAGTCA 
GACGAGCCGG CGATGATGTC ATCAGATTAT GACAGCACGT ATGAAACCAA CCACAGTGAC 
AGCAGCGACT TTGCACAGAC TGACGAGGAC AGAGAGGCTC GCAGGACGCT AACGCTAGCC 
ACGCTTATAC CAGCCGAGGA GAAGCCCATC TTGGCCCCGG AGCCGCTGCT GATGGAGGGA 
CTGGAGCCGC TTCTGTGTCA GATCAACAAC TGGAACTTTC CCATCTTCAG CCTGGTGGAG 
AAAACCAACG GCAGATGTGG CTGCATCCTC AGTCAGATGT CCTACCGACT GTTTGTCGAC 
ACGGGACTCT TTGAGACATT CAAGATTCCA GTGCGTGAGT TCATGAACTA TTTCCACGCG 
CTGGAGAATG GATACAGGGA CATCCCATAT CACAACAGAA TTCATGCCAC AGACGTCCTC 
CATGCAGTCT GGTACCTCAC TACACAACCT GTGCCTGGTT TACCCACACT GCGGACACTC 
GGCAGCTCCG ACTCTGACAG TGGACTCGCT CACGGCCGGA TGAAGTATCT GATGTCCAGG 
ACTAATGCAG TGGAGGAGGA AGGTTACGGC TGTCTTTCAG GCCTCATTCC TGCGCTGGAG 
CTCATGGCGC TGTACGTCGC TGCTGCAATG CATGATTATG ACCATCCTGG TCGGACCAAC 
GCCTTCCTGG TGGCCACCAG CGCTCCACAG GCTGTGCTAT ACAATGACCG TTCAGTGCTG 
GAGAATCATC ACGCTGCCTC CGCCTGGAAC CTCTTCATGT CGCGGCCCGA GTACAACTTT 
CTGGCCAGTT TGGACCACAT GGAGTTTAAA CGCTTTCGCT TCCTGGTCAT CGAAGCCATT 
CTGGCCACTG ACCTCAAGAA ACACTTTGAT TTCCTGGCCG AATTCAACGC CAAGGTGGGT 
GATGACGGCT GCTCAGGGAT CGACTGGACC AATGAGAACG ACCGTCTGCT GGTCTGTCAG 
ATGTGCATTA AGCTAGCAGA CATAAACGGG CCGCTGAAAT GCAAGGATCT TCATTTGCAG 
TGGACGGAGG GCATCGTCAA TGAGTTTTAC GAGCAGGGTG ATGAAGAGTC CAGTCTCGGT 
CTTCCCATCA GCCCCTTCAT GGACCGCTCA GCACCTCAGC TGGCCAAACT GCAAGAGTCC 
TTCATCACAC ACATCGTGGG TCCTCTGTGC AGCTCGTATG ACTCTGCGGC GCTCATGCCT 
GGCCGCTGGG TCCACCCTCT GCCCAAAGAT GGAGATGAGG TGGAGGACGA GAGCGAGGAG 
GAGGATGAGG ACACAGCGGA GGAGGAGGAA GAGGACGCCT CCACCAGCTC TGAACTCTCC 
CAAAAACCAG CACCTAAGAA ACGAAGGAAA GTCTTCTGCC AGATCTCCCA TCACCTGCTG 
GAGAACCATG AGATGTGGAA GAAGGTCATT GCAGAGGAGT CCCAGAGCCA GACTGAAGGG 
GAGGAGTCAA CATGCCTGAG CACCACCCCT GAGCCAATCC TGGCCATCGA TGAAGAGGAG 
GAGGAGCCAG GGAGCAAGGA GGAGCCAACG GATGACCAAG GGGAGGGGTC AACAGAGGAA 
GAGGAGGGAC CCGTACCTGA AGACACGCAT GAAACACAGG AACCAGATGG CGAGACTGTA 
GAGGAAGCAG ATGAGAAAAA CACAGAGACT GTAGAAGACA TTGAGGGAAA ACAAGACGAA 
GACCAGAAAG ATCCAGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDE3A ( XM_009299997.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009298272.1 Danio rerio phosphodiesterase 3A, cGMP-inhibited (pde3a), transcript variant X4, mRNA.

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CloneID	ODa45061
Clone ID Related Accession (Same CDS sequence)	XM_009299997.2 , XM_009299997.3
Accession Version	XM_009299997.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3321bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	cGMP-inhibited 3',5'-cyclic phosphodiesterase A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009299997.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACTGG AATCCGTGCG CAACGGCTAT GTGCGCACTT GTGTCACTCC CGTGGAGCAG 
GACTGCTGGC ACTCGTGCTT GCGAAGCCGT AGAAGCCTCT CGTCGGCCGT GTGCGTCGCC 
TCGCTGTCCG TGCTCCTCGC GCTGCTCTGG GACGCGGAGC GCACACACGC GCACTCGGGC 
TCTCACTTGG TGACGCTCGT GGAGCTCGTG TGGCAGCTGC TCTCGCCCGT CCTCACGCTC 
GTGTGTGCCT TCTTCTGGGT GGGTGTGTAT CTGCTCCGCT GCGGGGTGCT GATCCGCACG 
GCAGTGTTCC TGCTCACCGT GTGCCATCTG GGCGAAGCGG CGGCGCAGTC GCTGCTGGAT 
GCGCAGCAGC TGTACTCATT CACGGCCACC GCGCTGGTGC TGCTCGCCTG CCTGGCCAGC 
GGGGCGCTCA TGGTGGTGCG GCTGGGTCAG GGCATCTCCG TGCTGCTCTT CATCAGCATC 
ATCAGGACCG TGTCGCTTCT GTCGCTGCAG AAAGTGCGCG CCAGCTGGAG ACCGTACCTG 
GCGTACCTGC TCGGGCTGCT CGGGGTCCTG CTGGCGAGGT ATGCGGACAG GCTGCTGCCG 
GAGCAGGGCG CATCACACCG GGAGGGATCG AGGGGAGATA TTCCGGTATT TAAGAGGCGC 
AGGAGGTCCA GCTCCGTAGT CTCGTCAGAC ATGGCACAGC ACGGCCAGTC CAGAAGCAAA 
TCGCACAGGA GGACATCTCT GCCCTGCCTG CAGAGAGAAC AGATGCTCCC ACAGCAAGAA 
TGGGACAACA AAAGAGGCGC CCGAGGATCT CAGCAGCCAT CCGGCCTCAT GCAATCTTCG 
GGCACCAGTG TGACGGTGGA TATCGCGGTG ATGGGCGAGG CTCACGGACT GATCTCAGAC 
CTGCTGGCTG ACCAGTCGCT CGCACCCAAC ATCTGCAGCA CCCTGCGGGC CGTCAGTAAC 
CTGCTGAACA CCCAGCTCAC CTTCCAGCCC CTGCACCACC GGCCACGCCT GGGCCCCCTG 
CTGGCCTTCA GCGACGGACA CACGTGCTCC GACTCGGAGG AGGCGCCTGA GAAAGGAGAG 
AGGCTGGCTA TTCCAAAGCG TTTGAGGAGG AGTTTGCATC CAGGGCTTTT GAGACGGATT 
TCTTCCACGT GGACCACCAC CACCTCCGCC ACAGGCCTGC CAACCCTCGA ACCCGGGCCG 
GTCCGACGAG ACCGCAGCGC CAGCATCAAA GCACCTCACG ATGGCCCTTC CAGCAGTTTG 
GTGAACAGTG ATTCATGGAA CAACTCCAAT ATGCTCAGCA TTTCCAAGAG CAGGTCCATG 
TCTGCTTCAT ACGCTGCTTC CTCCAACACC ACCAACCACA TCTATAGCAA GAGCCGGAGA 
GTGCAGAGCT CTCCGCTGAC CAGCCCCACC AACAAGACCC CCTCAGTGGA GCTGCCAGAC 
CCCGAGCTTC CTCCACCACG CAGCCCACAT AAAGCCTTAA CCCACAGTCA GAGCGCACCC 
AGCTCCAGCG CAGCGCTCTG GCCACCGCCA ACGCCACTAT GTAGCAGCTG TGGACGGCCG 
TACAGTAAAC AGAGGCACAG TGATGGATCC ACAGAGCCAG CAGAGCGAAC ACAACAGTCA 
GACGAGCCGG CGATGATGTC ATCAGATTAT GACAGCACGT ATGAAACCAA CCACAGTGAC 
AGCAGCGACT TTGCACAGAC TGACGAGGAC AGAGAGGCTC GCAGGACGCT AACGCTAGCC 
ACGCTTATAC CAGCCGAGGA GAAGCCCATC TTGGCCCCGG AGCCGCTGCT GATGGAGGGA 
CTGGAGCCGC TTCTGTGTCA GATCAACAAC TGGAACTTTC CCATCTTCAG CCTGGTGGAG 
AAAACCAACG GCAGATGTGG CTGCATCCTC AGTCAGATGT CCTACCGACT GTTTGTCGAC 
ACGGGACTCT TTGAGACATT CAAGATTCCA GTGCGTGAGT TCATGAACTA TTTCCACGCG 
CTGGAGAATG GATACAGGGA CATCCCATAT CACAACAGAA TTCATGCCAC AGACGTCCTC 
CATGCAGTCT GGTACCTCAC TACACAACCT GTGCCTGGTT TACCCACACT GCGGACACTC 
GGCAGCTCCG ACTCTGACAG TGGACTCGCT CACGGCCGGA TGAAGTATCT GATGTCCAGG 
ACTAATGCAG TGGAGGAGGA AGGTTACGGC TGTCTTTCAG GCCTCATTCC TGCGCTGGAG 
CTCATGGCGC TGTACGTCGC TGCTGCAATG CATGATTATG ACCATCCTGG TCGGACCAAC 
GCCTTCCTGG TGGCCACCAG CGCTCCACAG GCTGTGCTAT ACAATGACCG TTCAGTGCTG 
GAGAATCATC ACGCTGCCTC CGCCTGGAAC CTCTTCATGT CGCGGCCCGA GTACAACTTT 
CTGGCCAGTT TGGACCACAT GGAGTTTAAA CGCTTTCGCT TCCTGGTCAT CGAAGCCATT 
CTGGCCACTG ACCTCAAGAA ACACTTTGAT TTCCTGGCCG AATTCAACGC CAAGGTGGGT 
GATGACGGCT GCTCAGGGAT CGACTGGACC AATGAGAACG ACCGTCTGCT GGTCTGTCAG 
ATGTGCATTA AGCTAGCAGA CATAAACGGG CCGCTGAAAT GCAAGGATCT TCATTTGCAG 
TGGACGGAGG GCATCGTCAA TGAGTTTTAC GAGCAGGGTG ATGAAGAGTC CAGTCTCGGT 
CTTCCCATCA GCCCCTTCAT GGACCGCTCA GCACCTCAGC TGGCCAAACT GCAAGAGTCC 
TTCATCACAC ACATCGTGGG TCCTCTGTGC AGCTCGTATG ACTCTGCGGC GCTCATGCCT 
GGCCGCTGGG TCCACCCTCT GCCCAAAGAT GGAGATGAGG TGGAGGACGA GAGCGAGGAG 
GAGGATGAGG ACACAGCGGA GGAGGAGGAA GAGGACGCCT CCACCAGCTC TGAACTCTCC 
CAAAAACCAG CACCTAAGAA ACGAAGGAAA GTCTTCTGCC AGATCTCCCA TCACCTGCTG 
GAGAACCATG AGATGTGGAA GAAGGTCATT GCAGAGGAGT CCCAGAGCCA GACTGAAGGG 
GAGGAGTCAA CATGCCTGAG CACCACCCCT GAGCCAATCC TGGCCATCGA TGAAGAGGAG 
GAGGAGCCAG GGAGCAAGGA GGAGCCAACG GATGACCAAG GGGAGGGGTC AACAGAGGAA 
GAGGAGGGAC CCGTACCTGA AGACACGCAT GAAACACAGG AACCAGATGG CGAGACTGTA 
GAGGAAGCAG ATGAGAAAAA CACAGAGACT GTAGAAGACA TTGAGGGAAA ACAAGACGAA 
GACCAGAAAG ATCCAGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298272.1
CDS	646..3966
Translation	MALESVRNGYVRTCVTPVEQDCWHSCLRSRRSLSSAVCVASLSVLLALLWDAERTHAHSGSHLVTLVELVWQLLSPVLTLVCAFFWVGVYLLRCGVLIRTAVFLLTVCHLGEAAAQSLLDAQQLYSFTATALVLLACLASGALMVVRLGQGISVLLFISIIRTVSLLSLQKVRASWRPYLAYLLGLLGVLLARYADRLLPEQGASHREGSRGDIPVFKRRRRSSSVVSSDMAQHGQSRSKSHRRTSLPCLQREQMLPQQEWDNKRGARGSQQPSGLMQSSGTSVTVDIAVMGEAHGLISDLLADQSLAPNICSTLRAVSNLLNTQLTFQPLHHRPRLGPLLAFSDGHTCSDSEEAPEKGERLAIPKRLRRSLHPGLLRRISSTWTTTTSATGLPTLEPGPVRRDRSASIKAPHDGPSSSLVNSDSWNNSNMLSISKSRSMSASYAASSNTTNHIYSKSRRVQSSPLTSPTNKTPSVELPDPELPPPRSPHKALTHSQSAPSSSAALWPPPTPLCSSCGRPYSKQRHSDGSTEPAERTQQSDEPAMMSSDYDSTYETNHSDSSDFAQTDEDREARRTLTLATLIPAEEKPILAPEPLLMEGLEPLLCQINNWNFPIFSLVEKTNGRCGCILSQMSYRLFVDTGLFETFKIPVREFMNYFHALENGYRDIPYHNRIHATDVLHAVWYLTTQPVPGLPTLRTLGSSDSDSGLAHGRMKYLMSRTNAVEEEGYGCLSGLIPALELMALYVAAAMHDYDHPGRTNAFLVATSAPQAVLYNDRSVLENHHAASAWNLFMSRPEYNFLASLDHMEFKRFRFLVIEAILATDLKKHFDFLAEFNAKVGDDGCSGIDWTNENDRLLVCQMCIKLADINGPLKCKDLHLQWTEGIVNEFYEQGDEESSLGLPISPFMDRSAPQLAKLQESFITHIVGPLCSSYDSAALMPGRWVHPLPKDGDEVEDESEEEDEDTAEEEEEDASTSSELSQKPAPKKRRKVFCQISHHLLENHEMWKKVIAEESQSQTEGEESTCLSTTPEPILAIDEEEEEPGSKEEPTDDQGEGSTEEEEGPVPEDTHETQEPDGETVEEADEKNTETVEDIEGKQDEDQKDPE

Target ORF information:

RefSeq Version	XM_009299997.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphodiesterase 3A, cGMP-inhibited (pde3a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009299997.3

ORF Insert Sequence:

ATGGCACTGG AATCCGTGCG CAACGGCTAT GTGCGCACTT GTGTCACTCC CGTGGAGCAG 
GACTGCTGGC ACTCGTGCTT GCGAAGCCGT AGAAGCCTCT CGTCGGCCGT GTGCGTCGCC 
TCGCTGTCCG TGCTCCTCGC GCTGCTCTGG GACGCGGAGC GCACACACGC GCACTCGGGC 
TCTCACTTGG TGACGCTCGT GGAGCTCGTG TGGCAGCTGC TCTCGCCCGT CCTCACGCTC 
GTGTGTGCCT TCTTCTGGGT GGGTGTGTAT CTGCTCCGCT GCGGGGTGCT GATCCGCACG 
GCAGTGTTCC TGCTCACCGT GTGCCATCTG GGCGAAGCGG CGGCGCAGTC GCTGCTGGAT 
GCGCAGCAGC TGTACTCATT CACGGCCACC GCGCTGGTGC TGCTCGCCTG CCTGGCCAGC 
GGGGCGCTCA TGGTGGTGCG GCTGGGTCAG GGCATCTCCG TGCTGCTCTT CATCAGCATC 
ATCAGGACCG TGTCGCTTCT GTCGCTGCAG AAAGTGCGCG CCAGCTGGAG ACCGTACCTG 
GCGTACCTGC TCGGGCTGCT CGGGGTCCTG CTGGCGAGGT ATGCGGACAG GCTGCTGCCG 
GAGCAGGGCG CATCACACCG GGAGGGATCG AGGGGAGATA TTCCGGTATT TAAGAGGCGC 
AGGAGGTCCA GCTCCGTAGT CTCGTCAGAC ATGGCACAGC ACGGCCAGTC CAGAAGCAAA 
TCGCACAGGA GGACATCTCT GCCCTGCCTG CAGAGAGAAC AGATGCTCCC ACAGCAAGAA 
TGGGACAACA AAAGAGGCGC CCGAGGATCT CAGCAGCCAT CCGGCCTCAT GCAATCTTCG 
GGCACCAGTG TGACGGTGGA TATCGCGGTG ATGGGCGAGG CTCACGGACT GATCTCAGAC 
CTGCTGGCTG ACCAGTCGCT CGCACCCAAC ATCTGCAGCA CCCTGCGGGC CGTCAGTAAC 
CTGCTGAACA CCCAGCTCAC CTTCCAGCCC CTGCACCACC GGCCACGCCT GGGCCCCCTG 
CTGGCCTTCA GCGACGGACA CACGTGCTCC GACTCGGAGG AGGCGCCTGA GAAAGGAGAG 
AGGCTGGCTA TTCCAAAGCG TTTGAGGAGG AGTTTGCATC CAGGGCTTTT GAGACGGATT 
TCTTCCACGT GGACCACCAC CACCTCCGCC ACAGGCCTGC CAACCCTCGA ACCCGGGCCG 
GTCCGACGAG ACCGCAGCGC CAGCATCAAA GCACCTCACG ATGGCCCTTC CAGCAGTTTG 
GTGAACAGTG ATTCATGGAA CAACTCCAAT ATGCTCAGCA TTTCCAAGAG CAGGTCCATG 
TCTGCTTCAT ACGCTGCTTC CTCCAACACC ACCAACCACA TCTATAGCAA GAGCCGGAGA 
GTGCAGAGCT CTCCGCTGAC CAGCCCCACC AACAAGACCC CCTCAGTGGA GCTGCCAGAC 
CCCGAGCTTC CTCCACCACG CAGCCCACAT AAAGCCTTAA CCCACAGTCA GAGCGCACCC 
AGCTCCAGCG CAGCGCTCTG GCCACCGCCA ACGCCACTAT GTAGCAGCTG TGGACGGCCG 
TACAGTAAAC AGAGGCACAG TGATGGATCC ACAGAGCCAG CAGAGCGAAC ACAACAGTCA 
GACGAGCCGG CGATGATGTC ATCAGATTAT GACAGCACGT ATGAAACCAA CCACAGTGAC 
AGCAGCGACT TTGCACAGAC TGACGAGGAC AGAGAGGCTC GCAGGACGCT AACGCTAGCC 
ACGCTTATAC CAGCCGAGGA GAAGCCCATC TTGGCCCCGG AGCCGCTGCT GATGGAGGGA 
CTGGAGCCGC TTCTGTGTCA GATCAACAAC TGGAACTTTC CCATCTTCAG CCTGGTGGAG 
AAAACCAACG GCAGATGTGG CTGCATCCTC AGTCAGATGT CCTACCGACT GTTTGTCGAC 
ACGGGACTCT TTGAGACATT CAAGATTCCA GTGCGTGAGT TCATGAACTA TTTCCACGCG 
CTGGAGAATG GATACAGGGA CATCCCATAT CACAACAGAA TTCATGCCAC AGACGTCCTC 
CATGCAGTCT GGTACCTCAC TACACAACCT GTGCCTGGTT TACCCACACT GCGGACACTC 
GGCAGCTCCG ACTCTGACAG TGGACTCGCT CACGGCCGGA TGAAGTATCT GATGTCCAGG 
ACTAATGCAG TGGAGGAGGA AGGTTACGGC TGTCTTTCAG GCCTCATTCC TGCGCTGGAG 
CTCATGGCGC TGTACGTCGC TGCTGCAATG CATGATTATG ACCATCCTGG TCGGACCAAC 
GCCTTCCTGG TGGCCACCAG CGCTCCACAG GCTGTGCTAT ACAATGACCG TTCAGTGCTG 
GAGAATCATC ACGCTGCCTC CGCCTGGAAC CTCTTCATGT CGCGGCCCGA GTACAACTTT 
CTGGCCAGTT TGGACCACAT GGAGTTTAAA CGCTTTCGCT TCCTGGTCAT CGAAGCCATT 
CTGGCCACTG ACCTCAAGAA ACACTTTGAT TTCCTGGCCG AATTCAACGC CAAGGTGGGT 
GATGACGGCT GCTCAGGGAT CGACTGGACC AATGAGAACG ACCGTCTGCT GGTCTGTCAG 
ATGTGCATTA AGCTAGCAGA CATAAACGGG CCGCTGAAAT GCAAGGATCT TCATTTGCAG 
TGGACGGAGG GCATCGTCAA TGAGTTTTAC GAGCAGGGTG ATGAAGAGTC CAGTCTCGGT 
CTTCCCATCA GCCCCTTCAT GGACCGCTCA GCACCTCAGC TGGCCAAACT GCAAGAGTCC 
TTCATCACAC ACATCGTGGG TCCTCTGTGC AGCTCGTATG ACTCTGCGGC GCTCATGCCT 
GGCCGCTGGG TCCACCCTCT GCCCAAAGAT GGAGATGAGG TGGAGGACGA GAGCGAGGAG 
GAGGATGAGG ACACAGCGGA GGAGGAGGAA GAGGACGCCT CCACCAGCTC TGAACTCTCC 
CAAAAACCAG CACCTAAGAA ACGAAGGAAA GTCTTCTGCC AGATCTCCCA TCACCTGCTG 
GAGAACCATG AGATGTGGAA GAAGGTCATT GCAGAGGAGT CCCAGAGCCA GACTGAAGGG 
GAGGAGTCAA CATGCCTGAG CACCACCCCT GAGCCAATCC TGGCCATCGA TGAAGAGGAG 
GAGGAGCCAG GGAGCAAGGA GGAGCCAACG GATGACCAAG GGGAGGGGTC AACAGAGGAA 
GAGGAGGGAC CCGTACCTGA AGACACGCAT GAAACACAGG AACCAGATGG CGAGACTGTA 
GAGGAAGCAG ATGAGAAAAA CACAGAGACT GTAGAAGACA TTGAGGGAAA ACAAGACGAA 
GACCAGAAAG ATCCAGAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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PDE3A cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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