mia3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mia3
Entrez Gene ID	567041
Full Name	melanoma inhibitory activity family, member 3
Synonyms	mia2,sb:eu1084,si:ch211-180b22.1,si:ch211-180b22.2,wu:fb05c09
General protein information	Preferred Names melanoma inhibitory activity family, member 3 Names melanoma inhibitory activity protein 3 melanoma inhibitory activity 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005160892.3	XP_005160949.1	melanoma inhibitory activity protein 3 isoform X1
XM_005160892.4	XP_005160949.1	melanoma inhibitory activity protein 3 isoform X1
XM_005160893.4	XP_005160950.1	melanoma inhibitory activity protein 3 isoform X2
XM_005160893.3	XP_005160950.1	melanoma inhibitory activity protein 3 isoform X2
XM_005160894.4	XP_005160951.1	melanoma inhibitory activity protein 3 isoform X3
XM_005160894.3	XP_005160951.1	melanoma inhibitory activity protein 3 isoform X3
XM_003200775.4	XP_003200823.1	melanoma inhibitory activity protein 3 isoform X4
XM_003200775.5	XP_003200823.1	melanoma inhibitory activity protein 3 isoform X4

Pathways from BioSystems
Gene Ontology

The following mia3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mia3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa25320	XM_003200775.5 Latest version!	Danio rerio melanoma inhibitory activity family, member 3 (mia3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa25317	XM_005160892.4 Latest version!	Danio rerio melanoma inhibitory activity family, member 3 (mia3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa25318	XM_005160893.4 Latest version!	Danio rerio melanoma inhibitory activity family, member 3 (mia3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa25319	XM_005160894.4 Latest version!	Danio rerio melanoma inhibitory activity family, member 3 (mia3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
View Old Accession Versions >>
ODa25317	XM_005160892.3	Danio rerio melanoma inhibitory activity family, member 3 (mia3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa25318	XM_005160893.3	Danio rerio melanoma inhibitory activity family, member 3 (mia3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa25319	XM_005160894.3	Danio rerio melanoma inhibitory activity family, member 3 (mia3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa25320	XM_003200775.4	Danio rerio melanoma inhibitory activity family, member 3 (mia3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mia3 ( XM_003200775.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003200823.1 Danio rerio melanoma inhibitory activity family, member 3 (mia3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa25320
Clone ID Related Accession (Same CDS sequence)	XM_003200775.4 , XM_003200775.5
Accession Version	XM_003200775.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	melanoma inhibitory activity protein 3 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003200775.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGG ACTCACAACC AATTCTTACC TCTCAACCAG CTGAGATAGA AAACACTGCC 
TTTTTCGTCT TCATCCTGTC CAGACTTCAC GAACTGATCA CTGCGCTTCC AGAGGAATGG 
CGACCGGGTC CCAATTTTCA CGGAGTCCCC TGGGAGCCAG TGGTGGTCAC AGTGGCCATC 
GGGGCCCTCA CTATACTGAT GTTCTTCTGG AGGACGGTGC TCGCTATTAA AGGCAGAAGC 
TATCAGTTAA CTGAAAAGCA GCTTAGAGAC AAAATCCAGC AGCTTCTCAA TGAGAAATCT 
GATGCTGCGA ACAAGATCAC AGAATTAAAT GACATGATAA AAGAAAGAGA ACAACGCCTG 
CAAAACTCAG AGAAGTCGCT GAGTTCCAGC CAACATGAAA TAAAAGGGCT GAAGAATCAT 
CACCAGAAAA TGCAGAGTCA GTGGGAGCAG ATGTCTGGGA GTATTTCGCA GCTCAATCAG 
ACAATTGTGG ACACACAGGA AGAAAACGCA AACCTTAATG AAAAGATTTC CAAGATGCAC 
CAGAGAATTG AGAAATACCA GAAAACACTG AAGAATTACG ATGATGAGCG TGCAAAGGTT 
CATGTCCTTA TGGATGAAGC TAAACTCCGA GAAGACGCTC TTAAGGCTCA GGTTCTGTCT 
TTTGAGAAGG AAAATGGTGC TTTAAAAGAG CAGAAGAAAT CTCTCCTTGG CGACGCTAAA 
GACTGGCAGG AGAAGCACAA GAAGCTGAGC GAGGAGATCC GAGTTTATCA CAAATCCCAG 
AAAGAGCTGG AAGACTCTCT GGTGCACAAG GAGAACGAGA TCGATGTTTT GTCCAGCTGT 
ATCGCCGAGC TCAACCGACT GGGAGGCTGC GACTCTGCAG AGCTACAGAA AGAAGATGCT 
AGAATGTCTA ACGGGGATGA ATCTGAGAAG AAAATGGACA CTATGAGACT GCGAATCAAA 
CAGATGATGG ACGTCTCCAG GATTAAAGCC ACTCTCAGCG TTGTGGAGGA CGAGAGGAAT 
CGCTATATGG AAAATCTCCT CGCTGAACAG AAATCCAGAC AAGAGCTGGA AGAGCAATAT 
CAGAAGGTCA TGCATGACCA GATGAATCTG AACAATGAGA AAACGCATCT GGAGAACCAG 
ATGAAGAACC TGCAGCAGAG ACTGGAGATC ACCACTGAAC TCTACCAGCA GAAGGAGAAC 
GCACTGCAAC AAAAGTTGAC TCAGGAGGAG CTGGAGAGAC GCGAGAAGGA GACGAAGCTG 
TCGGAGGTGG ACAGTAAAGC GCTGCGCTCG GAGGAGGAGG TTCGAGCTCT CAAGCAGAAG 
ATAAAGGACA TCGAGGAGGA GATGCAGCAG AACGAACGCT CACTCAAATC TGAGGTGGCA 
GTGCAAGAGA AGAAGGCCCA TGAGAACTGG TTAAAAGCAC GCGCCTCAGA GAGAACTCTG 
GTGGAGGAGA GAAGAGAATC AGCCACTCTT CGGCAGAAGC TTGTGGAATA CCGAGATAAA 
ATATCCGACA TGGAACAAAG TCTGTTTAAG TTGAACTCTG GGCCGCCAGA TCGACACATG 
CCGCCGCCAC AGCGCAGAGG GGATTCGTAC GGTCCATCTC CTGTGAGCGG CGGGGCTCCG 
TCTCCTCCTT TGATGATCGA GGGTCCAGGA CGGCCCCCAT CAGCACCTGT AGGGCGACGC 
GGTGAGCCGT TCGGTCCACG ACCCCCGTCT GATCCTCACG GACGCTTCTC TGAGCTTGGA 
CACCCGCTGC CATCCAGACC AGAAATGTTT CAGCCGATGA CATCATCTCC ATGTGCTCAT 
GATGGGCCGA TGCAGACGGC ACCAGTCGTG GAGCCAGCCG AGTCCTCAGA TCAGGCCTCA 
TCTGAGCCTG TAGAGTCTCT GTCCAAATCT CAGAGTCAGG GATCCTTCCT GCCCTCCCCG 
ATCCGAGATT CTCCGGTTCC TCCACCTAAC GTCCCGCTGA AGCCTTACGG TCCCCCAGGG 
ATGCCGCCCA ATGGGCCACC GATGATGATC CGACCACCCA ACGGACACCT TCCCATGATG 
CCCCCCGACC CGCGCTTCAG ACCTCCACAC ATGGACTCTT ACGGCCCGCC TCCACACATC 
GGCCCGTTTG GACCCGTGCC TCCACCTTTT GCTCGAGGGC CTCCACTGGG GCCGCCACTC 
GGACAGCGGG ACGTCCCGCC AGATTTCTTT GCACCCCGCG GCCTCCCTCC TCGGCCCTTT 
CCGCCTGGAC CTCTGCCACC CCCCGGAGCC ATGATCCCTC CTCCATACAG CGGACACCGT 
TTTCCAGCAG CCCCTGCAGT ACTGTCTCAG AGCTCCAGAG ATGGAGACGA GGAGCCGCCG 
CAGACCCACG GGGAGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003200823.1
CDS	287..2647
Translation	MRMDSQPILTSQPAEIENTAFFVFILSRLHELITALPEEWRPGPNFHGVPWEPVVVTVAIGALTILMFFWRTVLAIKGRSYQLTEKQLRDKIQQLLNEKSDAANKITELNDMIKEREQRLQNSEKSLSSSQHEIKGLKNHHQKMQSQWEQMSGSISQLNQTIVDTQEENANLNEKISKMHQRIEKYQKTLKNYDDERAKVHVLMDEAKLREDALKAQVLSFEKENGALKEQKKSLLGDAKDWQEKHKKLSEEIRVYHKSQKELEDSLVHKENEIDVLSSCIAELNRLGGCDSAELQKEDARMSNGDESEKKMDTMRLRIKQMMDVSRIKATLSVVEDERNRYMENLLAEQKSRQELEEQYQKVMHDQMNLNNEKTHLENQMKNLQQRLEITTELYQQKENALQQKLTQEELERREKETKLSEVDSKALRSEEEVRALKQKIKDIEEEMQQNERSLKSEVAVQEKKAHENWLKARASERTLVEERRESATLRQKLVEYRDKISDMEQSLFKLNSGPPDRHMPPPQRRGDSYGPSPVSGGAPSPPLMIEGPGRPPSAPVGRRGEPFGPRPPSDPHGRFSELGHPLPSRPEMFQPMTSSPCAHDGPMQTAPVVEPAESSDQASSEPVESLSKSQSQGSFLPSPIRDSPVPPPNVPLKPYGPPGMPPNGPPMMIRPPNGHLPMMPPDPRFRPPHMDSYGPPPHIGPFGPVPPPFARGPPLGPPLGQRDVPPDFFAPRGLPPRPFPPGPLPPPGAMIPPPYSGHRFPAAPAVLSQSSRDGDEEPPQTHGEP

Target ORF information:

RefSeq Version	XM_003200775.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio melanoma inhibitory activity family, member 3 (mia3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003200775.4

ORF Insert Sequence:

ATGAGGATGG ACTCACAACC AATTCTTACC TCTCAACCAG CTGAGATAGA AAACACTGCC 
TTTTTCGTCT TCATCCTGTC CAGACTTCAC GAACTGATCA CTGCGCTTCC AGAGGAATGG 
CGACCGGGTC CCAATTTTCA CGGAGTCCCC TGGGAGCCAG TGGTGGTCAC AGTGGCCATC 
GGGGCCCTCA CTATACTGAT GTTCTTCTGG AGGACGGTGC TCGCTATTAA AGGCAGAAGC 
TATCAGTTAA CTGAAAAGCA GCTTAGAGAC AAAATCCAGC AGCTTCTCAA TGAGAAATCT 
GATGCTGCGA ACAAGATCAC AGAATTAAAT GACATGATAA AAGAAAGAGA ACAACGCCTG 
CAAAACTCAG AGAAGTCGCT GAGTTCCAGC CAACATGAAA TAAAAGGGCT GAAGAATCAT 
CACCAGAAAA TGCAGAGTCA GTGGGAGCAG ATGTCTGGGA GTATTTCGCA GCTCAATCAG 
ACAATTGTGG ACACACAGGA AGAAAACGCA AACCTTAATG AAAAGATTTC CAAGATGCAC 
CAGAGAATTG AGAAATACCA GAAAACACTG AAGAATTACG ATGATGAGCG TGCAAAGGTT 
CATGTCCTTA TGGATGAAGC TAAACTCCGA GAAGACGCTC TTAAGGCTCA GGTTCTGTCT 
TTTGAGAAGG AAAATGGTGC TTTAAAAGAG CAGAAGAAAT CTCTCCTTGG CGACGCTAAA 
GACTGGCAGG AGAAGCACAA GAAGCTGAGC GAGGAGATCC GAGTTTATCA CAAATCCCAG 
AAAGAGCTGG AAGACTCTCT GGTGCACAAG GAGAACGAGA TCGATGTTTT GTCCAGCTGT 
ATCGCCGAGC TCAACCGACT GGGAGGCTGC GACTCTGCAG AGCTACAGAA AGAAGATGCT 
AGAATGTCTA ACGGGGATGA ATCTGAGAAG AAAATGGACA CTATGAGACT GCGAATCAAA 
CAGATGATGG ACGTCTCCAG GATTAAAGCC ACTCTCAGCG TTGTGGAGGA CGAGAGGAAT 
CGCTATATGG AAAATCTCCT CGCTGAACAG AAATCCAGAC AAGAGCTGGA AGAGCAATAT 
CAGAAGGTCA TGCATGACCA GATGAATCTG AACAATGAGA AAACGCATCT GGAGAACCAG 
ATGAAGAACC TGCAGCAGAG ACTGGAGATC ACCACTGAAC TCTACCAGCA GAAGGAGAAC 
GCACTGCAAC AAAAGTTGAC TCAGGAGGAG CTGGAGAGAC GCGAGAAGGA GACGAAGCTG 
TCGGAGGTGG ACAGTAAAGC GCTGCGCTCG GAGGAGGAGG TTCGAGCTCT CAAGCAGAAG 
ATAAAGGACA TCGAGGAGGA GATGCAGCAG AACGAACGCT CACTCAAATC TGAGGTGGCA 
GTGCAAGAGA AGAAGGCCCA TGAGAACTGG TTAAAAGCAC GCGCCTCAGA GAGAACTCTG 
GTGGAGGAGA GAAGAGAATC AGCCACTCTT CGGCAGAAGC TTGTGGAATA CCGAGATAAA 
ATATCCGACA TGGAACAAAG TCTGTTTAAG TTGAACTCTG GGCCGCCAGA TCGACACATG 
CCGCCGCCAC AGCGCAGAGG GGATTCGTAC GGTCCATCTC CTGTGAGCGG CGGGGCTCCG 
TCTCCTCCTT TGATGATCGA GGGTCCAGGA CGGCCCCCAT CAGCACCTGT AGGGCGACGC 
GGTGAGCCGT TCGGTCCACG ACCCCCGTCT GATCCTCACG GACGCTTCTC TGAGCTTGGA 
CACCCGCTGC CATCCAGACC AGAAATGTTT CAGCCGATGA CATCATCTCC ATGTGCTCAT 
GATGGGCCGA TGCAGACGGC ACCAGTCGTG GAGCCAGCCG AGTCCTCAGA TCAGGCCTCA 
TCTGAGCCTG TAGAGTCTCT GTCCAAATCT CAGAGTCAGG GATCCTTCCT GCCCTCCCCG 
ATCCGAGATT CTCCGGTTCC TCCACCTAAC GTCCCGCTGA AGCCTTACGG TCCCCCAGGG 
ATGCCGCCCA ATGGGCCACC GATGATGATC CGACCACCCA ACGGACACCT TCCCATGATG 
CCCCCCGACC CGCGCTTCAG ACCTCCACAC ATGGACTCTT ACGGCCCGCC TCCACACATC 
GGCCCGTTTG GACCCGTGCC TCCACCTTTT GCTCGAGGGC CTCCACTGGG GCCGCCACTC 
GGACAGCGGG ACGTCCCGCC AGATTTCTTT GCACCCCGCG GCCTCCCTCC TCGGCCCTTT 
CCGCCTGGAC CTCTGCCACC CCCCGGAGCC ATGATCCCTC CTCCATACAG CGGACACCGT 
TTTCCAGCAG CCCCTGCAGT ACTGTCTCAG AGCTCCAGAG ATGGAGACGA GGAGCCGCCG 
CAGACCCACG GGGAGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mia3 ( XM_003200775.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_003200823.1 Danio rerio melanoma inhibitory activity family, member 3 (mia3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa25320
Clone ID Related Accession (Same CDS sequence)	XM_003200775.4 , XM_003200775.5
Accession Version	XM_003200775.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	melanoma inhibitory activity protein 3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003200775.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGATGG ACTCACAACC AATTCTTACC TCTCAACCAG CTGAGATAGA AAACACTGCC 
TTTTTCGTCT TCATCCTGTC CAGACTTCAC GAACTGATCA CTGCGCTTCC AGAGGAATGG 
CGACCGGGTC CCAATTTTCA CGGAGTCCCC TGGGAGCCAG TGGTGGTCAC AGTGGCCATC 
GGGGCCCTCA CTATACTGAT GTTCTTCTGG AGGACGGTGC TCGCTATTAA AGGCAGAAGC 
TATCAGTTAA CTGAAAAGCA GCTTAGAGAC AAAATCCAGC AGCTTCTCAA TGAGAAATCT 
GATGCTGCGA ACAAGATCAC AGAATTAAAT GACATGATAA AAGAAAGAGA ACAACGCCTG 
CAAAACTCAG AGAAGTCGCT GAGTTCCAGC CAACATGAAA TAAAAGGGCT GAAGAATCAT 
CACCAGAAAA TGCAGAGTCA GTGGGAGCAG ATGTCTGGGA GTATTTCGCA GCTCAATCAG 
ACAATTGTGG ACACACAGGA AGAAAACGCA AACCTTAATG AAAAGATTTC CAAGATGCAC 
CAGAGAATTG AGAAATACCA GAAAACACTG AAGAATTACG ATGATGAGCG TGCAAAGGTT 
CATGTCCTTA TGGATGAAGC TAAACTCCGA GAAGACGCTC TTAAGGCTCA GGTTCTGTCT 
TTTGAGAAGG AAAATGGTGC TTTAAAAGAG CAGAAGAAAT CTCTCCTTGG CGACGCTAAA 
GACTGGCAGG AGAAGCACAA GAAGCTGAGC GAGGAGATCC GAGTTTATCA CAAATCCCAG 
AAAGAGCTGG AAGACTCTCT GGTGCACAAG GAGAACGAGA TCGATGTTTT GTCCAGCTGT 
ATCGCCGAGC TCAACCGACT GGGAGGCTGC GACTCTGCAG AGCTACAGAA AGAAGATGCT 
AGAATGTCTA ACGGGGATGA ATCTGAGAAG AAAATGGACA CTATGAGACT GCGAATCAAA 
CAGATGATGG ACGTCTCCAG GATTAAAGCC ACTCTCAGCG TTGTGGAGGA CGAGAGGAAT 
CGCTATATGG AAAATCTCCT CGCTGAACAG AAATCCAGAC AAGAGCTGGA AGAGCAATAT 
CAGAAGGTCA TGCATGACCA GATGAATCTG AACAATGAGA AAACGCATCT GGAGAACCAG 
ATGAAGAACC TGCAGCAGAG ACTGGAGATC ACCACTGAAC TCTACCAGCA GAAGGAGAAC 
GCACTGCAAC AAAAGTTGAC TCAGGAGGAG CTGGAGAGAC GCGAGAAGGA GACGAAGCTG 
TCGGAGGTGG ACAGTAAAGC GCTGCGCTCG GAGGAGGAGG TTCGAGCTCT CAAGCAGAAG 
ATAAAGGACA TCGAGGAGGA GATGCAGCAG AACGAACGCT CACTCAAATC TGAGGTGGCA 
GTGCAAGAGA AGAAGGCCCA TGAGAACTGG TTAAAAGCAC GCGCCTCAGA GAGAACTCTG 
GTGGAGGAGA GAAGAGAATC AGCCACTCTT CGGCAGAAGC TTGTGGAATA CCGAGATAAA 
ATATCCGACA TGGAACAAAG TCTGTTTAAG TTGAACTCTG GGCCGCCAGA TCGACACATG 
CCGCCGCCAC AGCGCAGAGG GGATTCGTAC GGTCCATCTC CTGTGAGCGG CGGGGCTCCG 
TCTCCTCCTT TGATGATCGA GGGTCCAGGA CGGCCCCCAT CAGCACCTGT AGGGCGACGC 
GGTGAGCCGT TCGGTCCACG ACCCCCGTCT GATCCTCACG GACGCTTCTC TGAGCTTGGA 
CACCCGCTGC CATCCAGACC AGAAATGTTT CAGCCGATGA CATCATCTCC ATGTGCTCAT 
GATGGGCCGA TGCAGACGGC ACCAGTCGTG GAGCCAGCCG AGTCCTCAGA TCAGGCCTCA 
TCTGAGCCTG TAGAGTCTCT GTCCAAATCT CAGAGTCAGG GATCCTTCCT GCCCTCCCCG 
ATCCGAGATT CTCCGGTTCC TCCACCTAAC GTCCCGCTGA AGCCTTACGG TCCCCCAGGG 
ATGCCGCCCA ATGGGCCACC GATGATGATC CGACCACCCA ACGGACACCT TCCCATGATG 
CCCCCCGACC CGCGCTTCAG ACCTCCACAC ATGGACTCTT ACGGCCCGCC TCCACACATC 
GGCCCGTTTG GACCCGTGCC TCCACCTTTT GCTCGAGGGC CTCCACTGGG GCCGCCACTC 
GGACAGCGGG ACGTCCCGCC AGATTTCTTT GCACCCCGCG GCCTCCCTCC TCGGCCCTTT 
CCGCCTGGAC CTCTGCCACC CCCCGGAGCC ATGATCCCTC CTCCATACAG CGGACACCGT 
TTTCCAGCAG CCCCTGCAGT ACTGTCTCAG AGCTCCAGAG ATGGAGACGA GGAGCCGCCG 
CAGACCCACG GGGAGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_003200823.1
CDS	292..2652
Translation	MRMDSQPILTSQPAEIENTAFFVFILSRLHELITALPEEWRPGPNFHGVPWEPVVVTVAIGALTILMFFWRTVLAIKGRSYQLTEKQLRDKIQQLLNEKSDAANKITELNDMIKEREQRLQNSEKSLSSSQHEIKGLKNHHQKMQSQWEQMSGSISQLNQTIVDTQEENANLNEKISKMHQRIEKYQKTLKNYDDERAKVHVLMDEAKLREDALKAQVLSFEKENGALKEQKKSLLGDAKDWQEKHKKLSEEIRVYHKSQKELEDSLVHKENEIDVLSSCIAELNRLGGCDSAELQKEDARMSNGDESEKKMDTMRLRIKQMMDVSRIKATLSVVEDERNRYMENLLAEQKSRQELEEQYQKVMHDQMNLNNEKTHLENQMKNLQQRLEITTELYQQKENALQQKLTQEELERREKETKLSEVDSKALRSEEEVRALKQKIKDIEEEMQQNERSLKSEVAVQEKKAHENWLKARASERTLVEERRESATLRQKLVEYRDKISDMEQSLFKLNSGPPDRHMPPPQRRGDSYGPSPVSGGAPSPPLMIEGPGRPPSAPVGRRGEPFGPRPPSDPHGRFSELGHPLPSRPEMFQPMTSSPCAHDGPMQTAPVVEPAESSDQASSEPVESLSKSQSQGSFLPSPIRDSPVPPPNVPLKPYGPPGMPPNGPPMMIRPPNGHLPMMPPDPRFRPPHMDSYGPPPHIGPFGPVPPPFARGPPLGPPLGQRDVPPDFFAPRGLPPRPFPPGPLPPPGAMIPPPYSGHRFPAAPAVLSQSSRDGDEEPPQTHGEP

Target ORF information:

RefSeq Version	XM_003200775.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio melanoma inhibitory activity family, member 3 (mia3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_003200775.5

ORF Insert Sequence:

ATGAGGATGG ACTCACAACC AATTCTTACC TCTCAACCAG CTGAGATAGA AAACACTGCC 
TTTTTCGTCT TCATCCTGTC CAGACTTCAC GAACTGATCA CTGCGCTTCC AGAGGAATGG 
CGACCGGGTC CCAATTTTCA CGGAGTCCCC TGGGAGCCAG TGGTGGTCAC AGTGGCCATC 
GGGGCCCTCA CTATACTGAT GTTCTTCTGG AGGACGGTGC TCGCTATTAA AGGCAGAAGC 
TATCAGTTAA CTGAAAAGCA GCTTAGAGAC AAAATCCAGC AGCTTCTCAA TGAGAAATCT 
GATGCTGCGA ACAAGATCAC AGAATTAAAT GACATGATAA AAGAAAGAGA ACAACGCCTG 
CAAAACTCAG AGAAGTCGCT GAGTTCCAGC CAACATGAAA TAAAAGGGCT GAAGAATCAT 
CACCAGAAAA TGCAGAGTCA GTGGGAGCAG ATGTCTGGGA GTATTTCGCA GCTCAATCAG 
ACAATTGTGG ACACACAGGA AGAAAACGCA AACCTTAATG AAAAGATTTC CAAGATGCAC 
CAGAGAATTG AGAAATACCA GAAAACACTG AAGAATTACG ATGATGAGCG TGCAAAGGTT 
CATGTCCTTA TGGATGAAGC TAAACTCCGA GAAGACGCTC TTAAGGCTCA GGTTCTGTCT 
TTTGAGAAGG AAAATGGTGC TTTAAAAGAG CAGAAGAAAT CTCTCCTTGG CGACGCTAAA 
GACTGGCAGG AGAAGCACAA GAAGCTGAGC GAGGAGATCC GAGTTTATCA CAAATCCCAG 
AAAGAGCTGG AAGACTCTCT GGTGCACAAG GAGAACGAGA TCGATGTTTT GTCCAGCTGT 
ATCGCCGAGC TCAACCGACT GGGAGGCTGC GACTCTGCAG AGCTACAGAA AGAAGATGCT 
AGAATGTCTA ACGGGGATGA ATCTGAGAAG AAAATGGACA CTATGAGACT GCGAATCAAA 
CAGATGATGG ACGTCTCCAG GATTAAAGCC ACTCTCAGCG TTGTGGAGGA CGAGAGGAAT 
CGCTATATGG AAAATCTCCT CGCTGAACAG AAATCCAGAC AAGAGCTGGA AGAGCAATAT 
CAGAAGGTCA TGCATGACCA GATGAATCTG AACAATGAGA AAACGCATCT GGAGAACCAG 
ATGAAGAACC TGCAGCAGAG ACTGGAGATC ACCACTGAAC TCTACCAGCA GAAGGAGAAC 
GCACTGCAAC AAAAGTTGAC TCAGGAGGAG CTGGAGAGAC GCGAGAAGGA GACGAAGCTG 
TCGGAGGTGG ACAGTAAAGC GCTGCGCTCG GAGGAGGAGG TTCGAGCTCT CAAGCAGAAG 
ATAAAGGACA TCGAGGAGGA GATGCAGCAG AACGAACGCT CACTCAAATC TGAGGTGGCA 
GTGCAAGAGA AGAAGGCCCA TGAGAACTGG TTAAAAGCAC GCGCCTCAGA GAGAACTCTG 
GTGGAGGAGA GAAGAGAATC AGCCACTCTT CGGCAGAAGC TTGTGGAATA CCGAGATAAA 
ATATCCGACA TGGAACAAAG TCTGTTTAAG TTGAACTCTG GGCCGCCAGA TCGACACATG 
CCGCCGCCAC AGCGCAGAGG GGATTCGTAC GGTCCATCTC CTGTGAGCGG CGGGGCTCCG 
TCTCCTCCTT TGATGATCGA GGGTCCAGGA CGGCCCCCAT CAGCACCTGT AGGGCGACGC 
GGTGAGCCGT TCGGTCCACG ACCCCCGTCT GATCCTCACG GACGCTTCTC TGAGCTTGGA 
CACCCGCTGC CATCCAGACC AGAAATGTTT CAGCCGATGA CATCATCTCC ATGTGCTCAT 
GATGGGCCGA TGCAGACGGC ACCAGTCGTG GAGCCAGCCG AGTCCTCAGA TCAGGCCTCA 
TCTGAGCCTG TAGAGTCTCT GTCCAAATCT CAGAGTCAGG GATCCTTCCT GCCCTCCCCG 
ATCCGAGATT CTCCGGTTCC TCCACCTAAC GTCCCGCTGA AGCCTTACGG TCCCCCAGGG 
ATGCCGCCCA ATGGGCCACC GATGATGATC CGACCACCCA ACGGACACCT TCCCATGATG 
CCCCCCGACC CGCGCTTCAG ACCTCCACAC ATGGACTCTT ACGGCCCGCC TCCACACATC 
GGCCCGTTTG GACCCGTGCC TCCACCTTTT GCTCGAGGGC CTCCACTGGG GCCGCCACTC 
GGACAGCGGG ACGTCCCGCC AGATTTCTTT GCACCCCGCG GCCTCCCTCC TCGGCCCTTT 
CCGCCTGGAC CTCTGCCACC CCCCGGAGCC ATGATCCCTC CTCCATACAG CGGACACCGT 
TTTCCAGCAG CCCCTGCAGT ACTGTCTCAG AGCTCCAGAG ATGGAGACGA GGAGCCGCCG 
CAGACCCACG GGGAGCCCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mia3 ( XM_005160892.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160949.1 Danio rerio melanoma inhibitory activity family, member 3 (mia3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25317
Clone ID Related Accession (Same CDS sequence)	XM_005160892.3 , XM_005160892.4
Accession Version	XM_005160892.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	melanoma inhibitory activity protein 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160892.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAC CGAATAATCC CCGGTTTTAC TCACTTTTTA CCCTCCTCCT GTGCTCGTGC 
TTCGGCGGGA GCTGCTCGGA CCGGCGGTTC TCGGAGCTGA AGCGCTGCGC GGATGAAGAA 
TGCAGCATGC TTCTCGGCAG AGGAAAGGCC GCAAAGGATT TCACGGGGCC GGACTGTCGG 
TTTCTCTCTT TTAAGAAAGG AGAGACGATA TATGTATATT ATAAACTCTC AGGGCAGAGG 
TCAGACGTGT GGGCTGGTAG TGTTGGAAAC CACTTTGGCT ATTTTCCAAA AGACTATCTT 
AATATAAATC ATATATATAC TGAAAAAGAA TTGGAGGTAC CTACAGAAGA AACAGATTTT 
GTTTGCTTTG AGACTGGGCT TGATAAATTT GAAAGTTATG ACATAGATGT GTTATTAGGA 
AGCAAATTAT TTATGGAAAT TGAGAATTCA ACAGAAGAAT CAAAAGAAGC AGATCCGTAT 
GACATTTCTG AAACAGAAGA ATCAGTGGAG CATCTTGATT CAGAATCTCT GCTTGAATCA 
GAGGCAGTTC ATTCAGAATC AAGATTGCCT CTTCAATCAG AGTTGATCGA TTCAGAATCA 
GTATTGATTC TCCAACAAGA GTCAGTTGAT TCTGAATCAA AATTGCCTCT TGAATTAGAA 
TCAGTCAATT TAGAATCAGC ATCGACTCAT AAGTCAGTTA ATTCAGAAAC AGAATCGACT 
CATGAATCAG AGTTTGTTTA TTCGAAACCT GCATTGACTC CTGAATCAGA GTCAGTTGAT 
TCAGAATCAA GAACAAAGTC AATTGATTCA GAATCAACAA CACAGTTAAC TAATTCAGAA 
TCAACAACAC AGTCAACTGA TTCAGAATCA AGAACACAGT CAACTGATTC AGAATCAACA 
AAACAGTCAA CTAATTCAGA ATCAAGAACA CAGTCAACTG ATTCAGAATC AATGTTAAAG 
TCAACCGATT CAGAATTAAC CACACAGTCA ACCGATTTAG AATCAACTGA TTCAGAATCA 
ACAGCAGAGT CAACTGATTC AGAATCAACA ACACAGTCGA ATGATTTAGA ATCAACATCA 
CAGTCAACGG ATTCAGAATC AACAGCAGAG TCAAATGATT CAGAATCAGC AACACGGTCA 
AATGATTCAG GATCAACATT ACAGTCAAAT GATTCAGAAT CAGCAACACA GTCAAATGAT 
TCAGGATCAA CATTACAGTC AAATGCTTCA GAATCAACGT CGCAGTCAGC AGATTCAGAA 
TCAACAACAC AGCCAATTGA TTCAGAATCA ACATTGGCTC TTGAATCAGA ATCATTTGAG 
TCAGAATTAA AATCATTACC TCTTGAGTCA GAGTCATTTG ATTCAGAATC AACACTGCCT 
CTTAAATCAG AGTCAGTAGA TTCAGAATCT GTAGAAGCTT CTGAATCAGA CACATTGATT 
CCTAAATCTA CTGAAGATGA CTCTGATAGC TCTCAGCCAA AGACCGTCGT CTCCTCAGAG 
TCAACACTGG AGCCTAAAGA AGCAGCGACA GAAGAGACAG ATACTTTAAC AAGCCAAGAT 
GATGTAGAAA CACAACCATC AGATTTTGAT GCACTAAACA TTGATGATGT TGAACTTTTG 
CACATCCAAC ATGACTCTGA TTCTGAGTCA CAGCTTGAAA CACAAACAGC AGGTTTAGAT 
GTAGATATCG AAGAGCCTGC TAGCCATAAA GATTTACCAC TACAAACTTT AGAGAAAACA 
CTTGAGGATG AGGACAGCTG GTCATCAGCA TTAAATGAAA AAAGTGAATC GAAAGAGACT 
GAATCTTTTC ACGAACACGC CAAAGAATCA CTTTTAGTTC ATGACCTCAA AGCACAAAGA 
GATTTTGAAG AAGAGGAAAA TATTGATGAT GTGGAATTTT CTCATTCTCC TCACAATACG 
AAATCTGCTT CAGATGAGAG TGAAGTCGTC CCATCTGAAC CAGCTCTGGA AACATTAGCA 
TTAAATATAA GTGAAATCAC AGAGAATACA AAGGAAAATG CAGATGATTT TGTTATGAAG 
GCCAGCATGA ACACCAATGA AAATGCACAC AAAAACATTG CAGACGATGC TCGGGATGCA 
GCGATAAACA GAAGCCAACA GCCTACAGAG TCCATCGATG AAGATGAAAC TGAGAACGTT 
TCTGAAAGCC CAGAATCTGC TCCTCTACTT TATAAAACAG ACGAACAGAT CGAAAAGGTG 
AAGAACGAAC TGGAGGATCT GCTTAAAAAC ACACTGGAGA CTGAGGAAGA TAGTCCTGGA 
GGTGAAGAGG ACGAGGCTGA AGAGCTTCTG GAAGACGAAA ACGCTCTTCT TTCATCTAAT 
AGTCCTCACC TCACAGACCA AAATCCTGAA GACAATGAGC AACATGATGA TGATGGTTTG 
GTTGAATCTC AACAAAGCGA AAACACAACA CAAGCAGATG AAACTGAAAC TGAAGAGGTT 
GGTCTTCCTC CTGAAGAGCC TGAATACAGC GATAGCGTAC TGAGACTCAC AATATTGCGA 
GATCACTTGA AGGATGAGGA TACCGAGCGC ATGCAGAAGT ACTTCGGCCT GAAGAACATG 
TTCAGAATCG AAGCCATGTT CTCTGATCTG GATCTTGAGA TGAAGTCTGC TCGGGAGATG 
AAGACGGATA CGGAGGAGAT CGAGCAAACG CTGGACCAGA TCACGGAGGC TTCAGAAAAC 
TCCATTCTCG ATGCCACAGA GGACATACTT AACGACAGAG ACCGGAAAGC TCTGGAGGAT 
GGACTGCAGA AGCAACCGGA GATGTATGAT GTTGAGGCCG CCATGTTGGA CGCTTTTCAG 
GAGATCGTTT TCGCTTTGCG ACAGAAATAT TCGACAGCCA GCGACAGCGT CCCATTGGTC 
GAGGAGGAAC AAAGTGCATA TGAAAGAGAT GACCCCCGAG CTGACGCTGA AGAGGTGAAC 
AATCTGGACA TTACAACTGA AATAACTGAG CCAGCTGAAA CTCCTGAAAT GCACAAGGAG 
CAGAATGAAT CTGAACCGAT TCTGAATCCT GAATTGAACG TGAACCCAGA TTTGTCCCAC 
AATAAAGATG TGGATCTTGA GGAGGACGGA GGCCATTTCA ACAGGAATAA AGACGCTCAA 
ATCAGTTTCG AAGACGCTGA GGAGATCCAA AAGGGACCTC ATGCTATTTT GGAGAAGCCA 
GTGGACATTG GCTTTCACTT TGAGATGGAT CCGTCTTCAG GTTCGTTGGA GTCTCCGAGT 
GTCTCCGATT TCCATGATGC AGAAACTGGC AGTGATTCCT CCGACTCATC GACTTCAGAT 
GAACTTCAGG ATTTGGTGGT GCTTGTCAAA GAAAATCTGG GAGTTTATGG AGAGATTCTG 
ATCACTGCGC TTCCAGAGGA ATGGCGACCG GGTCCCAATT TTCACGGAGT CCCCTGGGAG 
CCAGTGGTGG TCACAGTGGC CATCGGGGCC CTCACTATAC TGATGTTCTT CTGGAGGACG 
GTGCTCGCTA TTAAAGGCAG AAGCTATCAG TTAACTGAAA AGCAGCTTAG AGACAAAATC 
CAGCAGCTTC TCAATGAGAA ATCTGATGCT GCGAACAAGA TCACAGAATT AAATGACATG 
ATAAAAGAAA GAGAACAACG CCTGCAAAAC TCAGAGAAGT CGCTGAGTTC CAGCCAACAT 
GAAATAAAAG GGCTGAAGAA TCATCACCAG AAAATGCAGA GTCAGTGGGA GCAGATGTCT 
GGGAGTATTT CGCAGCTCAA TCAGACAATT GTGGACACAC AGGAAGAAAA CGCAAACCTT 
AATGAAAAGA TTTCCAAGAT GCACCAGAGA ATTGAGAAAT ACCAGAAAAC ACTGAAGAAT 
TACGATGATG AGCGTGCAAA GGTTCATGTC CTTATGGATG AAGCTAAACT CCGAGAAGAC 
GCTCTTAAGG CTCAGGTTCT GTCTTTTGAG AAGGAAAATG GTGCTTTAAA AGAGCAGAAG 
AAATCTCTCC TTGGCGACGC TAAAGACTGG CAGGAGAAGC ACAAGAAGCT GAGCGAGGAG 
ATCCGAGTTT ATCACAAATC CCAGAAAGAG CTGGAAGACT CTCTGGTGCA CAAGGAGAAC 
GAGATCGATG TTTTGTCCAG CTGTATCGCC GAGCTCAACC GACTGGGAGG CTGCGACTCT 
GCAGAGCTAC AGAAAGAAGA TGCTAGAATG TCTAACGGGG ATGAATCTGA GAAGAAAATG 
GACACTATGA GACTGCGAAT CAAACAGATG ATGGACGTCT CCAGGATTAA AGCCACTCTC 
AGCGTTGTGG AGGACGAGAG GAATCGCTAT ATGGAAAATC TCCTCGCTGA ACAGAAATCC 
AGACAAGAGC TGGAAGAGCA ATATCAGAAG GTCATGCATG ACCAGATGAA TCTGAACAAT 
GAGAAAACGC ATCTGGAGAA CCAGATGAAG AACCTGCAGC AGAGACTGGA GATCACCACT 
GAACTCTACC AGCAGAAGGA GAACGCACTG CAACAAAAGT TGACTCAGGA GGAGCTGGAG 
AGACGCGAGA AGGAGACGAA GCTGTCGGAG GTGGACAGTA AAGCGCTGCG CTCGGAGGAG 
GAGGTTCGAG CTCTCAAGCA GAAGATAAAG GACATCGAGG AGGAGATGCA GCAGAACGAA 
CGCTCACTCA AATCTGAGGT GGCAGTGCAA GAGAAGAAGG CCCATGAGAA CTGGTTAAAA 
GCACGCGCCT CAGAGAGAAC TCTGGTGGAG GAGAGAAGAG AATCAGCCAC TCTTCGGCAG 
AAGCTTGTGG AATACCGAGA TAAAATATCC GACATGGAAC AAAGTCTGTT TAAGTTGAAC 
TCTGGGCCGC CAGATCGACA CATGCCGCCG CCACAGCGCA GAGGGGATTC GTACGGTCCA 
TCTCCTGTGA GCGGCGGGGC TCCGTCTCCT CCTTTGATGA TCGAGGGTCC AGGACGGCCC 
CCATCAGCAC CTGTAGGGCG ACGCGGTGAG CCGTTCGGTC CACGACCCCC GTCTGATCCT 
CACGGACGCT TCTCTGAGCT TGGACACCCG CTGCCATCCA GACCAGAAAT GTTTCAGCCG 
ATGACATCAT CTCCATGTGC TCATGATGGG CCGATGCAGA CGGCACCAGT CGTGGAGCCA 
GCCGAGTCCT CAGATCAGGC CTCATCTGAG CCTGTAGAGT CTCTGTCCAA ATCTCAGAGT 
CAGGGATCCT TCCTGCCCTC CCCGATCCGA GATTCTCCGG TTCCTCCACC TAACGTCCCG 
CTGAAGCCTT ACGGTCCCCC AGGGATGCCG CCCAATGGGC CACCGATGAT GATCCGACCA 
CCCAACGGAC ACCTTCCCAT GATGCCCCCC GACCCGCGCT TCAGACCTCC ACACATGGAC 
TCTTACGGCC CGCCTCCACA CATCGGCCCG TTTGGACCCG TGCCTCCACC TTTTGCTCGA 
GGGCCTCCAC TGGGGCCGCC ACTCGGACAG CGGGACGTCC CGCCAGATTT CTTTGCACCC 
CGCGGCCTCC CTCCTCGGCC CTTTCCGCCT GGACCTCTGC CACCCCCCGG AGCCATGATC 
CCTCCTCCAT ACAGCGGACA CCGTTTTCCA GCAGCCCCTG CAGTACTGTC TCAGAGCTCC 
AGAGATGGAG ACGAGGAGCC GCCGCAGACC CACGGGGAGC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160949.1
CDS	172..5796
Translation	MAAPNNPRFYSLFTLLLCSCFGGSCSDRRFSELKRCADEECSMLLGRGKAAKDFTGPDCRFLSFKKGETIYVYYKLSGQRSDVWAGSVGNHFGYFPKDYLNINHIYTEKELEVPTEETDFVCFETGLDKFESYDIDVLLGSKLFMEIENSTEESKEADPYDISETEESVEHLDSESLLESEAVHSESRLPLQSELIDSESVLILQQESVDSESKLPLELESVNLESASTHKSVNSETESTHESEFVYSKPALTPESESVDSESRTKSIDSESTTQLTNSESTTQSTDSESRTQSTDSESTKQSTNSESRTQSTDSESMLKSTDSELTTQSTDLESTDSESTAESTDSESTTQSNDLESTSQSTDSESTAESNDSESATRSNDSGSTLQSNDSESATQSNDSGSTLQSNASESTSQSADSESTTQPIDSESTLALESESFESELKSLPLESESFDSESTLPLKSESVDSESVEASESDTLIPKSTEDDSDSSQPKTVVSSESTLEPKEAATEETDTLTSQDDVETQPSDFDALNIDDVELLHIQHDSDSESQLETQTAGLDVDIEEPASHKDLPLQTLEKTLEDEDSWSSALNEKSESKETESFHEHAKESLLVHDLKAQRDFEEEENIDDVEFSHSPHNTKSASDESEVVPSEPALETLALNISEITENTKENADDFVMKASMNTNENAHKNIADDARDAAINRSQQPTESIDEDETENVSESPESAPLLYKTDEQIEKVKNELEDLLKNTLETEEDSPGGEEDEAEELLEDENALLSSNSPHLTDQNPEDNEQHDDDGLVESQQSENTTQADETETEEVGLPPEEPEYSDSVLRLTILRDHLKDEDTERMQKYFGLKNMFRIEAMFSDLDLEMKSAREMKTDTEEIEQTLDQITEASENSILDATEDILNDRDRKALEDGLQKQPEMYDVEAAMLDAFQEIVFALRQKYSTASDSVPLVEEEQSAYERDDPRADAEEVNNLDITTEITEPAETPEMHKEQNESEPILNPELNVNPDLSHNKDVDLEEDGGHFNRNKDAQISFEDAEEIQKGPHAILEKPVDIGFHFEMDPSSGSLESPSVSDFHDAETGSDSSDSSTSDELQDLVVLVKENLGVYGEILITALPEEWRPGPNFHGVPWEPVVVTVAIGALTILMFFWRTVLAIKGRSYQLTEKQLRDKIQQLLNEKSDAANKITELNDMIKEREQRLQNSEKSLSSSQHEIKGLKNHHQKMQSQWEQMSGSISQLNQTIVDTQEENANLNEKISKMHQRIEKYQKTLKNYDDERAKVHVLMDEAKLREDALKAQVLSFEKENGALKEQKKSLLGDAKDWQEKHKKLSEEIRVYHKSQKELEDSLVHKENEIDVLSSCIAELNRLGGCDSAELQKEDARMSNGDESEKKMDTMRLRIKQMMDVSRIKATLSVVEDERNRYMENLLAEQKSRQELEEQYQKVMHDQMNLNNEKTHLENQMKNLQQRLEITTELYQQKENALQQKLTQEELERREKETKLSEVDSKALRSEEEVRALKQKIKDIEEEMQQNERSLKSEVAVQEKKAHENWLKARASERTLVEERRESATLRQKLVEYRDKISDMEQSLFKLNSGPPDRHMPPPQRRGDSYGPSPVSGGAPSPPLMIEGPGRPPSAPVGRRGEPFGPRPPSDPHGRFSELGHPLPSRPEMFQPMTSSPCAHDGPMQTAPVVEPAESSDQASSEPVESLSKSQSQGSFLPSPIRDSPVPPPNVPLKPYGPPGMPPNGPPMMIRPPNGHLPMMPPDPRFRPPHMDSYGPPPHIGPFGPVPPPFARGPPLGPPLGQRDVPPDFFAPRGLPPRPFPPGPLPPPGAMIPPPYSGHRFPAAPAVLSQSSRDGDEEPPQTHGEP

Target ORF information:

RefSeq Version	XM_005160892.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio melanoma inhibitory activity family, member 3 (mia3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160892.3

ORF Insert Sequence:

ATGGCTGCAC CGAATAATCC CCGGTTTTAC TCACTTTTTA CCCTCCTCCT GTGCTCGTGC 
TTCGGCGGGA GCTGCTCGGA CCGGCGGTTC TCGGAGCTGA AGCGCTGCGC GGATGAAGAA 
TGCAGCATGC TTCTCGGCAG AGGAAAGGCC GCAAAGGATT TCACGGGGCC GGACTGTCGG 
TTTCTCTCTT TTAAGAAAGG AGAGACGATA TATGTATATT ATAAACTCTC AGGGCAGAGG 
TCAGACGTGT GGGCTGGTAG TGTTGGAAAC CACTTTGGCT ATTTTCCAAA AGACTATCTT 
AATATAAATC ATATATATAC TGAAAAAGAA TTGGAGGTAC CTACAGAAGA AACAGATTTT 
GTTTGCTTTG AGACTGGGCT TGATAAATTT GAAAGTTATG ACATAGATGT GTTATTAGGA 
AGCAAATTAT TTATGGAAAT TGAGAATTCA ACAGAAGAAT CAAAAGAAGC AGATCCGTAT 
GACATTTCTG AAACAGAAGA ATCAGTGGAG CATCTTGATT CAGAATCTCT GCTTGAATCA 
GAGGCAGTTC ATTCAGAATC AAGATTGCCT CTTCAATCAG AGTTGATCGA TTCAGAATCA 
GTATTGATTC TCCAACAAGA GTCAGTTGAT TCTGAATCAA AATTGCCTCT TGAATTAGAA 
TCAGTCAATT TAGAATCAGC ATCGACTCAT AAGTCAGTTA ATTCAGAAAC AGAATCGACT 
CATGAATCAG AGTTTGTTTA TTCGAAACCT GCATTGACTC CTGAATCAGA GTCAGTTGAT 
TCAGAATCAA GAACAAAGTC AATTGATTCA GAATCAACAA CACAGTTAAC TAATTCAGAA 
TCAACAACAC AGTCAACTGA TTCAGAATCA AGAACACAGT CAACTGATTC AGAATCAACA 
AAACAGTCAA CTAATTCAGA ATCAAGAACA CAGTCAACTG ATTCAGAATC AATGTTAAAG 
TCAACCGATT CAGAATTAAC CACACAGTCA ACCGATTTAG AATCAACTGA TTCAGAATCA 
ACAGCAGAGT CAACTGATTC AGAATCAACA ACACAGTCGA ATGATTTAGA ATCAACATCA 
CAGTCAACGG ATTCAGAATC AACAGCAGAG TCAAATGATT CAGAATCAGC AACACGGTCA 
AATGATTCAG GATCAACATT ACAGTCAAAT GATTCAGAAT CAGCAACACA GTCAAATGAT 
TCAGGATCAA CATTACAGTC AAATGCTTCA GAATCAACGT CGCAGTCAGC AGATTCAGAA 
TCAACAACAC AGCCAATTGA TTCAGAATCA ACATTGGCTC TTGAATCAGA ATCATTTGAG 
TCAGAATTAA AATCATTACC TCTTGAGTCA GAGTCATTTG ATTCAGAATC AACACTGCCT 
CTTAAATCAG AGTCAGTAGA TTCAGAATCT GTAGAAGCTT CTGAATCAGA CACATTGATT 
CCTAAATCTA CTGAAGATGA CTCTGATAGC TCTCAGCCAA AGACCGTCGT CTCCTCAGAG 
TCAACACTGG AGCCTAAAGA AGCAGCGACA GAAGAGACAG ATACTTTAAC AAGCCAAGAT 
GATGTAGAAA CACAACCATC AGATTTTGAT GCACTAAACA TTGATGATGT TGAACTTTTG 
CACATCCAAC ATGACTCTGA TTCTGAGTCA CAGCTTGAAA CACAAACAGC AGGTTTAGAT 
GTAGATATCG AAGAGCCTGC TAGCCATAAA GATTTACCAC TACAAACTTT AGAGAAAACA 
CTTGAGGATG AGGACAGCTG GTCATCAGCA TTAAATGAAA AAAGTGAATC GAAAGAGACT 
GAATCTTTTC ACGAACACGC CAAAGAATCA CTTTTAGTTC ATGACCTCAA AGCACAAAGA 
GATTTTGAAG AAGAGGAAAA TATTGATGAT GTGGAATTTT CTCATTCTCC TCACAATACG 
AAATCTGCTT CAGATGAGAG TGAAGTCGTC CCATCTGAAC CAGCTCTGGA AACATTAGCA 
TTAAATATAA GTGAAATCAC AGAGAATACA AAGGAAAATG CAGATGATTT TGTTATGAAG 
GCCAGCATGA ACACCAATGA AAATGCACAC AAAAACATTG CAGACGATGC TCGGGATGCA 
GCGATAAACA GAAGCCAACA GCCTACAGAG TCCATCGATG AAGATGAAAC TGAGAACGTT 
TCTGAAAGCC CAGAATCTGC TCCTCTACTT TATAAAACAG ACGAACAGAT CGAAAAGGTG 
AAGAACGAAC TGGAGGATCT GCTTAAAAAC ACACTGGAGA CTGAGGAAGA TAGTCCTGGA 
GGTGAAGAGG ACGAGGCTGA AGAGCTTCTG GAAGACGAAA ACGCTCTTCT TTCATCTAAT 
AGTCCTCACC TCACAGACCA AAATCCTGAA GACAATGAGC AACATGATGA TGATGGTTTG 
GTTGAATCTC AACAAAGCGA AAACACAACA CAAGCAGATG AAACTGAAAC TGAAGAGGTT 
GGTCTTCCTC CTGAAGAGCC TGAATACAGC GATAGCGTAC TGAGACTCAC AATATTGCGA 
GATCACTTGA AGGATGAGGA TACCGAGCGC ATGCAGAAGT ACTTCGGCCT GAAGAACATG 
TTCAGAATCG AAGCCATGTT CTCTGATCTG GATCTTGAGA TGAAGTCTGC TCGGGAGATG 
AAGACGGATA CGGAGGAGAT CGAGCAAACG CTGGACCAGA TCACGGAGGC TTCAGAAAAC 
TCCATTCTCG ATGCCACAGA GGACATACTT AACGACAGAG ACCGGAAAGC TCTGGAGGAT 
GGACTGCAGA AGCAACCGGA GATGTATGAT GTTGAGGCCG CCATGTTGGA CGCTTTTCAG 
GAGATCGTTT TCGCTTTGCG ACAGAAATAT TCGACAGCCA GCGACAGCGT CCCATTGGTC 
GAGGAGGAAC AAAGTGCATA TGAAAGAGAT GACCCCCGAG CTGACGCTGA AGAGGTGAAC 
AATCTGGACA TTACAACTGA AATAACTGAG CCAGCTGAAA CTCCTGAAAT GCACAAGGAG 
CAGAATGAAT CTGAACCGAT TCTGAATCCT GAATTGAACG TGAACCCAGA TTTGTCCCAC 
AATAAAGATG TGGATCTTGA GGAGGACGGA GGCCATTTCA ACAGGAATAA AGACGCTCAA 
ATCAGTTTCG AAGACGCTGA GGAGATCCAA AAGGGACCTC ATGCTATTTT GGAGAAGCCA 
GTGGACATTG GCTTTCACTT TGAGATGGAT CCGTCTTCAG GTTCGTTGGA GTCTCCGAGT 
GTCTCCGATT TCCATGATGC AGAAACTGGC AGTGATTCCT CCGACTCATC GACTTCAGAT 
GAACTTCAGG ATTTGGTGGT GCTTGTCAAA GAAAATCTGG GAGTTTATGG AGAGATTCTG 
ATCACTGCGC TTCCAGAGGA ATGGCGACCG GGTCCCAATT TTCACGGAGT CCCCTGGGAG 
CCAGTGGTGG TCACAGTGGC CATCGGGGCC CTCACTATAC TGATGTTCTT CTGGAGGACG 
GTGCTCGCTA TTAAAGGCAG AAGCTATCAG TTAACTGAAA AGCAGCTTAG AGACAAAATC 
CAGCAGCTTC TCAATGAGAA ATCTGATGCT GCGAACAAGA TCACAGAATT AAATGACATG 
ATAAAAGAAA GAGAACAACG CCTGCAAAAC TCAGAGAAGT CGCTGAGTTC CAGCCAACAT 
GAAATAAAAG GGCTGAAGAA TCATCACCAG AAAATGCAGA GTCAGTGGGA GCAGATGTCT 
GGGAGTATTT CGCAGCTCAA TCAGACAATT GTGGACACAC AGGAAGAAAA CGCAAACCTT 
AATGAAAAGA TTTCCAAGAT GCACCAGAGA ATTGAGAAAT ACCAGAAAAC ACTGAAGAAT 
TACGATGATG AGCGTGCAAA GGTTCATGTC CTTATGGATG AAGCTAAACT CCGAGAAGAC 
GCTCTTAAGG CTCAGGTTCT GTCTTTTGAG AAGGAAAATG GTGCTTTAAA AGAGCAGAAG 
AAATCTCTCC TTGGCGACGC TAAAGACTGG CAGGAGAAGC ACAAGAAGCT GAGCGAGGAG 
ATCCGAGTTT ATCACAAATC CCAGAAAGAG CTGGAAGACT CTCTGGTGCA CAAGGAGAAC 
GAGATCGATG TTTTGTCCAG CTGTATCGCC GAGCTCAACC GACTGGGAGG CTGCGACTCT 
GCAGAGCTAC AGAAAGAAGA TGCTAGAATG TCTAACGGGG ATGAATCTGA GAAGAAAATG 
GACACTATGA GACTGCGAAT CAAACAGATG ATGGACGTCT CCAGGATTAA AGCCACTCTC 
AGCGTTGTGG AGGACGAGAG GAATCGCTAT ATGGAAAATC TCCTCGCTGA ACAGAAATCC 
AGACAAGAGC TGGAAGAGCA ATATCAGAAG GTCATGCATG ACCAGATGAA TCTGAACAAT 
GAGAAAACGC ATCTGGAGAA CCAGATGAAG AACCTGCAGC AGAGACTGGA GATCACCACT 
GAACTCTACC AGCAGAAGGA GAACGCACTG CAACAAAAGT TGACTCAGGA GGAGCTGGAG 
AGACGCGAGA AGGAGACGAA GCTGTCGGAG GTGGACAGTA AAGCGCTGCG CTCGGAGGAG 
GAGGTTCGAG CTCTCAAGCA GAAGATAAAG GACATCGAGG AGGAGATGCA GCAGAACGAA 
CGCTCACTCA AATCTGAGGT GGCAGTGCAA GAGAAGAAGG CCCATGAGAA CTGGTTAAAA 
GCACGCGCCT CAGAGAGAAC TCTGGTGGAG GAGAGAAGAG AATCAGCCAC TCTTCGGCAG 
AAGCTTGTGG AATACCGAGA TAAAATATCC GACATGGAAC AAAGTCTGTT TAAGTTGAAC 
TCTGGGCCGC CAGATCGACA CATGCCGCCG CCACAGCGCA GAGGGGATTC GTACGGTCCA 
TCTCCTGTGA GCGGCGGGGC TCCGTCTCCT CCTTTGATGA TCGAGGGTCC AGGACGGCCC 
CCATCAGCAC CTGTAGGGCG ACGCGGTGAG CCGTTCGGTC CACGACCCCC GTCTGATCCT 
CACGGACGCT TCTCTGAGCT TGGACACCCG CTGCCATCCA GACCAGAAAT GTTTCAGCCG 
ATGACATCAT CTCCATGTGC TCATGATGGG CCGATGCAGA CGGCACCAGT CGTGGAGCCA 
GCCGAGTCCT CAGATCAGGC CTCATCTGAG CCTGTAGAGT CTCTGTCCAA ATCTCAGAGT 
CAGGGATCCT TCCTGCCCTC CCCGATCCGA GATTCTCCGG TTCCTCCACC TAACGTCCCG 
CTGAAGCCTT ACGGTCCCCC AGGGATGCCG CCCAATGGGC CACCGATGAT GATCCGACCA 
CCCAACGGAC ACCTTCCCAT GATGCCCCCC GACCCGCGCT TCAGACCTCC ACACATGGAC 
TCTTACGGCC CGCCTCCACA CATCGGCCCG TTTGGACCCG TGCCTCCACC TTTTGCTCGA 
GGGCCTCCAC TGGGGCCGCC ACTCGGACAG CGGGACGTCC CGCCAGATTT CTTTGCACCC 
CGCGGCCTCC CTCCTCGGCC CTTTCCGCCT GGACCTCTGC CACCCCCCGG AGCCATGATC 
CCTCCTCCAT ACAGCGGACA CCGTTTTCCA GCAGCCCCTG CAGTACTGTC TCAGAGCTCC 
AGAGATGGAG ACGAGGAGCC GCCGCAGACC CACGGGGAGC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mia3 ( XM_005160892.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160949.1 Danio rerio melanoma inhibitory activity family, member 3 (mia3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa25317
Clone ID Related Accession (Same CDS sequence)	XM_005160892.3 , XM_005160892.4
Accession Version	XM_005160892.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5625bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	melanoma inhibitory activity protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160892.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAC CGAATAATCC CCGGTTTTAC TCACTTTTTA CCCTCCTCCT GTGCTCGTGC 
TTCGGCGGGA GCTGCTCGGA CCGGCGGTTC TCGGAGCTGA AGCGCTGCGC GGATGAAGAA 
TGCAGCATGC TTCTCGGCAG AGGAAAGGCC GCAAAGGATT TCACGGGGCC GGACTGTCGG 
TTTCTCTCTT TTAAGAAAGG AGAGACGATA TATGTATATT ATAAACTCTC AGGGCAGAGG 
TCAGACGTGT GGGCTGGTAG TGTTGGAAAC CACTTTGGCT ATTTTCCAAA AGACTATCTT 
AATATAAATC ATATATATAC TGAAAAAGAA TTGGAGGTAC CTACAGAAGA AACAGATTTT 
GTTTGCTTTG AGACTGGGCT TGATAAATTT GAAAGTTATG ACATAGATGT GTTATTAGGA 
AGCAAATTAT TTATGGAAAT TGAGAATTCA ACAGAAGAAT CAAAAGAAGC AGATCCGTAT 
GACATTTCTG AAACAGAAGA ATCAGTGGAG CATCTTGATT CAGAATCTCT GCTTGAATCA 
GAGGCAGTTC ATTCAGAATC AAGATTGCCT CTTCAATCAG AGTTGATCGA TTCAGAATCA 
GTATTGATTC TCCAACAAGA GTCAGTTGAT TCTGAATCAA AATTGCCTCT TGAATTAGAA 
TCAGTCAATT TAGAATCAGC ATCGACTCAT AAGTCAGTTA ATTCAGAAAC AGAATCGACT 
CATGAATCAG AGTTTGTTTA TTCGAAACCT GCATTGACTC CTGAATCAGA GTCAGTTGAT 
TCAGAATCAA GAACAAAGTC AATTGATTCA GAATCAACAA CACAGTTAAC TAATTCAGAA 
TCAACAACAC AGTCAACTGA TTCAGAATCA AGAACACAGT CAACTGATTC AGAATCAACA 
AAACAGTCAA CTAATTCAGA ATCAAGAACA CAGTCAACTG ATTCAGAATC AATGTTAAAG 
TCAACCGATT CAGAATTAAC CACACAGTCA ACCGATTTAG AATCAACTGA TTCAGAATCA 
ACAGCAGAGT CAACTGATTC AGAATCAACA ACACAGTCGA ATGATTTAGA ATCAACATCA 
CAGTCAACGG ATTCAGAATC AACAGCAGAG TCAAATGATT CAGAATCAGC AACACGGTCA 
AATGATTCAG GATCAACATT ACAGTCAAAT GATTCAGAAT CAGCAACACA GTCAAATGAT 
TCAGGATCAA CATTACAGTC AAATGCTTCA GAATCAACGT CGCAGTCAGC AGATTCAGAA 
TCAACAACAC AGCCAATTGA TTCAGAATCA ACATTGGCTC TTGAATCAGA ATCATTTGAG 
TCAGAATTAA AATCATTACC TCTTGAGTCA GAGTCATTTG ATTCAGAATC AACACTGCCT 
CTTAAATCAG AGTCAGTAGA TTCAGAATCT GTAGAAGCTT CTGAATCAGA CACATTGATT 
CCTAAATCTA CTGAAGATGA CTCTGATAGC TCTCAGCCAA AGACCGTCGT CTCCTCAGAG 
TCAACACTGG AGCCTAAAGA AGCAGCGACA GAAGAGACAG ATACTTTAAC AAGCCAAGAT 
GATGTAGAAA CACAACCATC AGATTTTGAT GCACTAAACA TTGATGATGT TGAACTTTTG 
CACATCCAAC ATGACTCTGA TTCTGAGTCA CAGCTTGAAA CACAAACAGC AGGTTTAGAT 
GTAGATATCG AAGAGCCTGC TAGCCATAAA GATTTACCAC TACAAACTTT AGAGAAAACA 
CTTGAGGATG AGGACAGCTG GTCATCAGCA TTAAATGAAA AAAGTGAATC GAAAGAGACT 
GAATCTTTTC ACGAACACGC CAAAGAATCA CTTTTAGTTC ATGACCTCAA AGCACAAAGA 
GATTTTGAAG AAGAGGAAAA TATTGATGAT GTGGAATTTT CTCATTCTCC TCACAATACG 
AAATCTGCTT CAGATGAGAG TGAAGTCGTC CCATCTGAAC CAGCTCTGGA AACATTAGCA 
TTAAATATAA GTGAAATCAC AGAGAATACA AAGGAAAATG CAGATGATTT TGTTATGAAG 
GCCAGCATGA ACACCAATGA AAATGCACAC AAAAACATTG CAGACGATGC TCGGGATGCA 
GCGATAAACA GAAGCCAACA GCCTACAGAG TCCATCGATG AAGATGAAAC TGAGAACGTT 
TCTGAAAGCC CAGAATCTGC TCCTCTACTT TATAAAACAG ACGAACAGAT CGAAAAGGTG 
AAGAACGAAC TGGAGGATCT GCTTAAAAAC ACACTGGAGA CTGAGGAAGA TAGTCCTGGA 
GGTGAAGAGG ACGAGGCTGA AGAGCTTCTG GAAGACGAAA ACGCTCTTCT TTCATCTAAT 
AGTCCTCACC TCACAGACCA AAATCCTGAA GACAATGAGC AACATGATGA TGATGGTTTG 
GTTGAATCTC AACAAAGCGA AAACACAACA CAAGCAGATG AAACTGAAAC TGAAGAGGTT 
GGTCTTCCTC CTGAAGAGCC TGAATACAGC GATAGCGTAC TGAGACTCAC AATATTGCGA 
GATCACTTGA AGGATGAGGA TACCGAGCGC ATGCAGAAGT ACTTCGGCCT GAAGAACATG 
TTCAGAATCG AAGCCATGTT CTCTGATCTG GATCTTGAGA TGAAGTCTGC TCGGGAGATG 
AAGACGGATA CGGAGGAGAT CGAGCAAACG CTGGACCAGA TCACGGAGGC TTCAGAAAAC 
TCCATTCTCG ATGCCACAGA GGACATACTT AACGACAGAG ACCGGAAAGC TCTGGAGGAT 
GGACTGCAGA AGCAACCGGA GATGTATGAT GTTGAGGCCG CCATGTTGGA CGCTTTTCAG 
GAGATCGTTT TCGCTTTGCG ACAGAAATAT TCGACAGCCA GCGACAGCGT CCCATTGGTC 
GAGGAGGAAC AAAGTGCATA TGAAAGAGAT GACCCCCGAG CTGACGCTGA AGAGGTGAAC 
AATCTGGACA TTACAACTGA AATAACTGAG CCAGCTGAAA CTCCTGAAAT GCACAAGGAG 
CAGAATGAAT CTGAACCGAT TCTGAATCCT GAATTGAACG TGAACCCAGA TTTGTCCCAC 
AATAAAGATG TGGATCTTGA GGAGGACGGA GGCCATTTCA ACAGGAATAA AGACGCTCAA 
ATCAGTTTCG AAGACGCTGA GGAGATCCAA AAGGGACCTC ATGCTATTTT GGAGAAGCCA 
GTGGACATTG GCTTTCACTT TGAGATGGAT CCGTCTTCAG GTTCGTTGGA GTCTCCGAGT 
GTCTCCGATT TCCATGATGC AGAAACTGGC AGTGATTCCT CCGACTCATC GACTTCAGAT 
GAACTTCAGG ATTTGGTGGT GCTTGTCAAA GAAAATCTGG GAGTTTATGG AGAGATTCTG 
ATCACTGCGC TTCCAGAGGA ATGGCGACCG GGTCCCAATT TTCACGGAGT CCCCTGGGAG 
CCAGTGGTGG TCACAGTGGC CATCGGGGCC CTCACTATAC TGATGTTCTT CTGGAGGACG 
GTGCTCGCTA TTAAAGGCAG AAGCTATCAG TTAACTGAAA AGCAGCTTAG AGACAAAATC 
CAGCAGCTTC TCAATGAGAA ATCTGATGCT GCGAACAAGA TCACAGAATT AAATGACATG 
ATAAAAGAAA GAGAACAACG CCTGCAAAAC TCAGAGAAGT CGCTGAGTTC CAGCCAACAT 
GAAATAAAAG GGCTGAAGAA TCATCACCAG AAAATGCAGA GTCAGTGGGA GCAGATGTCT 
GGGAGTATTT CGCAGCTCAA TCAGACAATT GTGGACACAC AGGAAGAAAA CGCAAACCTT 
AATGAAAAGA TTTCCAAGAT GCACCAGAGA ATTGAGAAAT ACCAGAAAAC ACTGAAGAAT 
TACGATGATG AGCGTGCAAA GGTTCATGTC CTTATGGATG AAGCTAAACT CCGAGAAGAC 
GCTCTTAAGG CTCAGGTTCT GTCTTTTGAG AAGGAAAATG GTGCTTTAAA AGAGCAGAAG 
AAATCTCTCC TTGGCGACGC TAAAGACTGG CAGGAGAAGC ACAAGAAGCT GAGCGAGGAG 
ATCCGAGTTT ATCACAAATC CCAGAAAGAG CTGGAAGACT CTCTGGTGCA CAAGGAGAAC 
GAGATCGATG TTTTGTCCAG CTGTATCGCC GAGCTCAACC GACTGGGAGG CTGCGACTCT 
GCAGAGCTAC AGAAAGAAGA TGCTAGAATG TCTAACGGGG ATGAATCTGA GAAGAAAATG 
GACACTATGA GACTGCGAAT CAAACAGATG ATGGACGTCT CCAGGATTAA AGCCACTCTC 
AGCGTTGTGG AGGACGAGAG GAATCGCTAT ATGGAAAATC TCCTCGCTGA ACAGAAATCC 
AGACAAGAGC TGGAAGAGCA ATATCAGAAG GTCATGCATG ACCAGATGAA TCTGAACAAT 
GAGAAAACGC ATCTGGAGAA CCAGATGAAG AACCTGCAGC AGAGACTGGA GATCACCACT 
GAACTCTACC AGCAGAAGGA GAACGCACTG CAACAAAAGT TGACTCAGGA GGAGCTGGAG 
AGACGCGAGA AGGAGACGAA GCTGTCGGAG GTGGACAGTA AAGCGCTGCG CTCGGAGGAG 
GAGGTTCGAG CTCTCAAGCA GAAGATAAAG GACATCGAGG AGGAGATGCA GCAGAACGAA 
CGCTCACTCA AATCTGAGGT GGCAGTGCAA GAGAAGAAGG CCCATGAGAA CTGGTTAAAA 
GCACGCGCCT CAGAGAGAAC TCTGGTGGAG GAGAGAAGAG AATCAGCCAC TCTTCGGCAG 
AAGCTTGTGG AATACCGAGA TAAAATATCC GACATGGAAC AAAGTCTGTT TAAGTTGAAC 
TCTGGGCCGC CAGATCGACA CATGCCGCCG CCACAGCGCA GAGGGGATTC GTACGGTCCA 
TCTCCTGTGA GCGGCGGGGC TCCGTCTCCT CCTTTGATGA TCGAGGGTCC AGGACGGCCC 
CCATCAGCAC CTGTAGGGCG ACGCGGTGAG CCGTTCGGTC CACGACCCCC GTCTGATCCT 
CACGGACGCT TCTCTGAGCT TGGACACCCG CTGCCATCCA GACCAGAAAT GTTTCAGCCG 
ATGACATCAT CTCCATGTGC TCATGATGGG CCGATGCAGA CGGCACCAGT CGTGGAGCCA 
GCCGAGTCCT CAGATCAGGC CTCATCTGAG CCTGTAGAGT CTCTGTCCAA ATCTCAGAGT 
CAGGGATCCT TCCTGCCCTC CCCGATCCGA GATTCTCCGG TTCCTCCACC TAACGTCCCG 
CTGAAGCCTT ACGGTCCCCC AGGGATGCCG CCCAATGGGC CACCGATGAT GATCCGACCA 
CCCAACGGAC ACCTTCCCAT GATGCCCCCC GACCCGCGCT TCAGACCTCC ACACATGGAC 
TCTTACGGCC CGCCTCCACA CATCGGCCCG TTTGGACCCG TGCCTCCACC TTTTGCTCGA 
GGGCCTCCAC TGGGGCCGCC ACTCGGACAG CGGGACGTCC CGCCAGATTT CTTTGCACCC 
CGCGGCCTCC CTCCTCGGCC CTTTCCGCCT GGACCTCTGC CACCCCCCGG AGCCATGATC 
CCTCCTCCAT ACAGCGGACA CCGTTTTCCA GCAGCCCCTG CAGTACTGTC TCAGAGCTCC 
AGAGATGGAG ACGAGGAGCC GCCGCAGACC CACGGGGAGC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160949.1
CDS	152..5776
Translation	MAAPNNPRFYSLFTLLLCSCFGGSCSDRRFSELKRCADEECSMLLGRGKAAKDFTGPDCRFLSFKKGETIYVYYKLSGQRSDVWAGSVGNHFGYFPKDYLNINHIYTEKELEVPTEETDFVCFETGLDKFESYDIDVLLGSKLFMEIENSTEESKEADPYDISETEESVEHLDSESLLESEAVHSESRLPLQSELIDSESVLILQQESVDSESKLPLELESVNLESASTHKSVNSETESTHESEFVYSKPALTPESESVDSESRTKSIDSESTTQLTNSESTTQSTDSESRTQSTDSESTKQSTNSESRTQSTDSESMLKSTDSELTTQSTDLESTDSESTAESTDSESTTQSNDLESTSQSTDSESTAESNDSESATRSNDSGSTLQSNDSESATQSNDSGSTLQSNASESTSQSADSESTTQPIDSESTLALESESFESELKSLPLESESFDSESTLPLKSESVDSESVEASESDTLIPKSTEDDSDSSQPKTVVSSESTLEPKEAATEETDTLTSQDDVETQPSDFDALNIDDVELLHIQHDSDSESQLETQTAGLDVDIEEPASHKDLPLQTLEKTLEDEDSWSSALNEKSESKETESFHEHAKESLLVHDLKAQRDFEEEENIDDVEFSHSPHNTKSASDESEVVPSEPALETLALNISEITENTKENADDFVMKASMNTNENAHKNIADDARDAAINRSQQPTESIDEDETENVSESPESAPLLYKTDEQIEKVKNELEDLLKNTLETEEDSPGGEEDEAEELLEDENALLSSNSPHLTDQNPEDNEQHDDDGLVESQQSENTTQADETETEEVGLPPEEPEYSDSVLRLTILRDHLKDEDTERMQKYFGLKNMFRIEAMFSDLDLEMKSAREMKTDTEEIEQTLDQITEASENSILDATEDILNDRDRKALEDGLQKQPEMYDVEAAMLDAFQEIVFALRQKYSTASDSVPLVEEEQSAYERDDPRADAEEVNNLDITTEITEPAETPEMHKEQNESEPILNPELNVNPDLSHNKDVDLEEDGGHFNRNKDAQISFEDAEEIQKGPHAILEKPVDIGFHFEMDPSSGSLESPSVSDFHDAETGSDSSDSSTSDELQDLVVLVKENLGVYGEILITALPEEWRPGPNFHGVPWEPVVVTVAIGALTILMFFWRTVLAIKGRSYQLTEKQLRDKIQQLLNEKSDAANKITELNDMIKEREQRLQNSEKSLSSSQHEIKGLKNHHQKMQSQWEQMSGSISQLNQTIVDTQEENANLNEKISKMHQRIEKYQKTLKNYDDERAKVHVLMDEAKLREDALKAQVLSFEKENGALKEQKKSLLGDAKDWQEKHKKLSEEIRVYHKSQKELEDSLVHKENEIDVLSSCIAELNRLGGCDSAELQKEDARMSNGDESEKKMDTMRLRIKQMMDVSRIKATLSVVEDERNRYMENLLAEQKSRQELEEQYQKVMHDQMNLNNEKTHLENQMKNLQQRLEITTELYQQKENALQQKLTQEELERREKETKLSEVDSKALRSEEEVRALKQKIKDIEEEMQQNERSLKSEVAVQEKKAHENWLKARASERTLVEERRESATLRQKLVEYRDKISDMEQSLFKLNSGPPDRHMPPPQRRGDSYGPSPVSGGAPSPPLMIEGPGRPPSAPVGRRGEPFGPRPPSDPHGRFSELGHPLPSRPEMFQPMTSSPCAHDGPMQTAPVVEPAESSDQASSEPVESLSKSQSQGSFLPSPIRDSPVPPPNVPLKPYGPPGMPPNGPPMMIRPPNGHLPMMPPDPRFRPPHMDSYGPPPHIGPFGPVPPPFARGPPLGPPLGQRDVPPDFFAPRGLPPRPFPPGPLPPPGAMIPPPYSGHRFPAAPAVLSQSSRDGDEEPPQTHGEP

Target ORF information:

RefSeq Version	XM_005160892.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio melanoma inhibitory activity family, member 3 (mia3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160892.4

ORF Insert Sequence:

ATGGCTGCAC CGAATAATCC CCGGTTTTAC TCACTTTTTA CCCTCCTCCT GTGCTCGTGC 
TTCGGCGGGA GCTGCTCGGA CCGGCGGTTC TCGGAGCTGA AGCGCTGCGC GGATGAAGAA 
TGCAGCATGC TTCTCGGCAG AGGAAAGGCC GCAAAGGATT TCACGGGGCC GGACTGTCGG 
TTTCTCTCTT TTAAGAAAGG AGAGACGATA TATGTATATT ATAAACTCTC AGGGCAGAGG 
TCAGACGTGT GGGCTGGTAG TGTTGGAAAC CACTTTGGCT ATTTTCCAAA AGACTATCTT 
AATATAAATC ATATATATAC TGAAAAAGAA TTGGAGGTAC CTACAGAAGA AACAGATTTT 
GTTTGCTTTG AGACTGGGCT TGATAAATTT GAAAGTTATG ACATAGATGT GTTATTAGGA 
AGCAAATTAT TTATGGAAAT TGAGAATTCA ACAGAAGAAT CAAAAGAAGC AGATCCGTAT 
GACATTTCTG AAACAGAAGA ATCAGTGGAG CATCTTGATT CAGAATCTCT GCTTGAATCA 
GAGGCAGTTC ATTCAGAATC AAGATTGCCT CTTCAATCAG AGTTGATCGA TTCAGAATCA 
GTATTGATTC TCCAACAAGA GTCAGTTGAT TCTGAATCAA AATTGCCTCT TGAATTAGAA 
TCAGTCAATT TAGAATCAGC ATCGACTCAT AAGTCAGTTA ATTCAGAAAC AGAATCGACT 
CATGAATCAG AGTTTGTTTA TTCGAAACCT GCATTGACTC CTGAATCAGA GTCAGTTGAT 
TCAGAATCAA GAACAAAGTC AATTGATTCA GAATCAACAA CACAGTTAAC TAATTCAGAA 
TCAACAACAC AGTCAACTGA TTCAGAATCA AGAACACAGT CAACTGATTC AGAATCAACA 
AAACAGTCAA CTAATTCAGA ATCAAGAACA CAGTCAACTG ATTCAGAATC AATGTTAAAG 
TCAACCGATT CAGAATTAAC CACACAGTCA ACCGATTTAG AATCAACTGA TTCAGAATCA 
ACAGCAGAGT CAACTGATTC AGAATCAACA ACACAGTCGA ATGATTTAGA ATCAACATCA 
CAGTCAACGG ATTCAGAATC AACAGCAGAG TCAAATGATT CAGAATCAGC AACACGGTCA 
AATGATTCAG GATCAACATT ACAGTCAAAT GATTCAGAAT CAGCAACACA GTCAAATGAT 
TCAGGATCAA CATTACAGTC AAATGCTTCA GAATCAACGT CGCAGTCAGC AGATTCAGAA 
TCAACAACAC AGCCAATTGA TTCAGAATCA ACATTGGCTC TTGAATCAGA ATCATTTGAG 
TCAGAATTAA AATCATTACC TCTTGAGTCA GAGTCATTTG ATTCAGAATC AACACTGCCT 
CTTAAATCAG AGTCAGTAGA TTCAGAATCT GTAGAAGCTT CTGAATCAGA CACATTGATT 
CCTAAATCTA CTGAAGATGA CTCTGATAGC TCTCAGCCAA AGACCGTCGT CTCCTCAGAG 
TCAACACTGG AGCCTAAAGA AGCAGCGACA GAAGAGACAG ATACTTTAAC AAGCCAAGAT 
GATGTAGAAA CACAACCATC AGATTTTGAT GCACTAAACA TTGATGATGT TGAACTTTTG 
CACATCCAAC ATGACTCTGA TTCTGAGTCA CAGCTTGAAA CACAAACAGC AGGTTTAGAT 
GTAGATATCG AAGAGCCTGC TAGCCATAAA GATTTACCAC TACAAACTTT AGAGAAAACA 
CTTGAGGATG AGGACAGCTG GTCATCAGCA TTAAATGAAA AAAGTGAATC GAAAGAGACT 
GAATCTTTTC ACGAACACGC CAAAGAATCA CTTTTAGTTC ATGACCTCAA AGCACAAAGA 
GATTTTGAAG AAGAGGAAAA TATTGATGAT GTGGAATTTT CTCATTCTCC TCACAATACG 
AAATCTGCTT CAGATGAGAG TGAAGTCGTC CCATCTGAAC CAGCTCTGGA AACATTAGCA 
TTAAATATAA GTGAAATCAC AGAGAATACA AAGGAAAATG CAGATGATTT TGTTATGAAG 
GCCAGCATGA ACACCAATGA AAATGCACAC AAAAACATTG CAGACGATGC TCGGGATGCA 
GCGATAAACA GAAGCCAACA GCCTACAGAG TCCATCGATG AAGATGAAAC TGAGAACGTT 
TCTGAAAGCC CAGAATCTGC TCCTCTACTT TATAAAACAG ACGAACAGAT CGAAAAGGTG 
AAGAACGAAC TGGAGGATCT GCTTAAAAAC ACACTGGAGA CTGAGGAAGA TAGTCCTGGA 
GGTGAAGAGG ACGAGGCTGA AGAGCTTCTG GAAGACGAAA ACGCTCTTCT TTCATCTAAT 
AGTCCTCACC TCACAGACCA AAATCCTGAA GACAATGAGC AACATGATGA TGATGGTTTG 
GTTGAATCTC AACAAAGCGA AAACACAACA CAAGCAGATG AAACTGAAAC TGAAGAGGTT 
GGTCTTCCTC CTGAAGAGCC TGAATACAGC GATAGCGTAC TGAGACTCAC AATATTGCGA 
GATCACTTGA AGGATGAGGA TACCGAGCGC ATGCAGAAGT ACTTCGGCCT GAAGAACATG 
TTCAGAATCG AAGCCATGTT CTCTGATCTG GATCTTGAGA TGAAGTCTGC TCGGGAGATG 
AAGACGGATA CGGAGGAGAT CGAGCAAACG CTGGACCAGA TCACGGAGGC TTCAGAAAAC 
TCCATTCTCG ATGCCACAGA GGACATACTT AACGACAGAG ACCGGAAAGC TCTGGAGGAT 
GGACTGCAGA AGCAACCGGA GATGTATGAT GTTGAGGCCG CCATGTTGGA CGCTTTTCAG 
GAGATCGTTT TCGCTTTGCG ACAGAAATAT TCGACAGCCA GCGACAGCGT CCCATTGGTC 
GAGGAGGAAC AAAGTGCATA TGAAAGAGAT GACCCCCGAG CTGACGCTGA AGAGGTGAAC 
AATCTGGACA TTACAACTGA AATAACTGAG CCAGCTGAAA CTCCTGAAAT GCACAAGGAG 
CAGAATGAAT CTGAACCGAT TCTGAATCCT GAATTGAACG TGAACCCAGA TTTGTCCCAC 
AATAAAGATG TGGATCTTGA GGAGGACGGA GGCCATTTCA ACAGGAATAA AGACGCTCAA 
ATCAGTTTCG AAGACGCTGA GGAGATCCAA AAGGGACCTC ATGCTATTTT GGAGAAGCCA 
GTGGACATTG GCTTTCACTT TGAGATGGAT CCGTCTTCAG GTTCGTTGGA GTCTCCGAGT 
GTCTCCGATT TCCATGATGC AGAAACTGGC AGTGATTCCT CCGACTCATC GACTTCAGAT 
GAACTTCAGG ATTTGGTGGT GCTTGTCAAA GAAAATCTGG GAGTTTATGG AGAGATTCTG 
ATCACTGCGC TTCCAGAGGA ATGGCGACCG GGTCCCAATT TTCACGGAGT CCCCTGGGAG 
CCAGTGGTGG TCACAGTGGC CATCGGGGCC CTCACTATAC TGATGTTCTT CTGGAGGACG 
GTGCTCGCTA TTAAAGGCAG AAGCTATCAG TTAACTGAAA AGCAGCTTAG AGACAAAATC 
CAGCAGCTTC TCAATGAGAA ATCTGATGCT GCGAACAAGA TCACAGAATT AAATGACATG 
ATAAAAGAAA GAGAACAACG CCTGCAAAAC TCAGAGAAGT CGCTGAGTTC CAGCCAACAT 
GAAATAAAAG GGCTGAAGAA TCATCACCAG AAAATGCAGA GTCAGTGGGA GCAGATGTCT 
GGGAGTATTT CGCAGCTCAA TCAGACAATT GTGGACACAC AGGAAGAAAA CGCAAACCTT 
AATGAAAAGA TTTCCAAGAT GCACCAGAGA ATTGAGAAAT ACCAGAAAAC ACTGAAGAAT 
TACGATGATG AGCGTGCAAA GGTTCATGTC CTTATGGATG AAGCTAAACT CCGAGAAGAC 
GCTCTTAAGG CTCAGGTTCT GTCTTTTGAG AAGGAAAATG GTGCTTTAAA AGAGCAGAAG 
AAATCTCTCC TTGGCGACGC TAAAGACTGG CAGGAGAAGC ACAAGAAGCT GAGCGAGGAG 
ATCCGAGTTT ATCACAAATC CCAGAAAGAG CTGGAAGACT CTCTGGTGCA CAAGGAGAAC 
GAGATCGATG TTTTGTCCAG CTGTATCGCC GAGCTCAACC GACTGGGAGG CTGCGACTCT 
GCAGAGCTAC AGAAAGAAGA TGCTAGAATG TCTAACGGGG ATGAATCTGA GAAGAAAATG 
GACACTATGA GACTGCGAAT CAAACAGATG ATGGACGTCT CCAGGATTAA AGCCACTCTC 
AGCGTTGTGG AGGACGAGAG GAATCGCTAT ATGGAAAATC TCCTCGCTGA ACAGAAATCC 
AGACAAGAGC TGGAAGAGCA ATATCAGAAG GTCATGCATG ACCAGATGAA TCTGAACAAT 
GAGAAAACGC ATCTGGAGAA CCAGATGAAG AACCTGCAGC AGAGACTGGA GATCACCACT 
GAACTCTACC AGCAGAAGGA GAACGCACTG CAACAAAAGT TGACTCAGGA GGAGCTGGAG 
AGACGCGAGA AGGAGACGAA GCTGTCGGAG GTGGACAGTA AAGCGCTGCG CTCGGAGGAG 
GAGGTTCGAG CTCTCAAGCA GAAGATAAAG GACATCGAGG AGGAGATGCA GCAGAACGAA 
CGCTCACTCA AATCTGAGGT GGCAGTGCAA GAGAAGAAGG CCCATGAGAA CTGGTTAAAA 
GCACGCGCCT CAGAGAGAAC TCTGGTGGAG GAGAGAAGAG AATCAGCCAC TCTTCGGCAG 
AAGCTTGTGG AATACCGAGA TAAAATATCC GACATGGAAC AAAGTCTGTT TAAGTTGAAC 
TCTGGGCCGC CAGATCGACA CATGCCGCCG CCACAGCGCA GAGGGGATTC GTACGGTCCA 
TCTCCTGTGA GCGGCGGGGC TCCGTCTCCT CCTTTGATGA TCGAGGGTCC AGGACGGCCC 
CCATCAGCAC CTGTAGGGCG ACGCGGTGAG CCGTTCGGTC CACGACCCCC GTCTGATCCT 
CACGGACGCT TCTCTGAGCT TGGACACCCG CTGCCATCCA GACCAGAAAT GTTTCAGCCG 
ATGACATCAT CTCCATGTGC TCATGATGGG CCGATGCAGA CGGCACCAGT CGTGGAGCCA 
GCCGAGTCCT CAGATCAGGC CTCATCTGAG CCTGTAGAGT CTCTGTCCAA ATCTCAGAGT 
CAGGGATCCT TCCTGCCCTC CCCGATCCGA GATTCTCCGG TTCCTCCACC TAACGTCCCG 
CTGAAGCCTT ACGGTCCCCC AGGGATGCCG CCCAATGGGC CACCGATGAT GATCCGACCA 
CCCAACGGAC ACCTTCCCAT GATGCCCCCC GACCCGCGCT TCAGACCTCC ACACATGGAC 
TCTTACGGCC CGCCTCCACA CATCGGCCCG TTTGGACCCG TGCCTCCACC TTTTGCTCGA 
GGGCCTCCAC TGGGGCCGCC ACTCGGACAG CGGGACGTCC CGCCAGATTT CTTTGCACCC 
CGCGGCCTCC CTCCTCGGCC CTTTCCGCCT GGACCTCTGC CACCCCCCGG AGCCATGATC 
CCTCCTCCAT ACAGCGGACA CCGTTTTCCA GCAGCCCCTG CAGTACTGTC TCAGAGCTCC 
AGAGATGGAG ACGAGGAGCC GCCGCAGACC CACGGGGAGC CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mia3 ( XM_005160893.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160950.1 Danio rerio melanoma inhibitory activity family, member 3 (mia3), transcript variant X2, mRNA.

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CloneID	ODa25318
Clone ID Related Accession (Same CDS sequence)	XM_005160893.4 , XM_005160893.3
Accession Version	XM_005160893.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5622bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	melanoma inhibitory activity protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160893.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAC CGAATAATCC CCGGTTTTAC TCACTTTTTA CCCTCCTCCT GTGCTCGTGC 
TTCGGCGGGA GCTGCTCGGA CCGGCGGTTC TCGGAGCTGA AGCGCTGCGC GGATGAAGAA 
TGCAGCATGC TTCTCGGCAG AGGAAAGGCC GCAAAGGATT TCACGGGGCC GGACTGTCGG 
TTTCTCTCTT TTAAGAAAGG AGAGACGATA TATGTATATT ATAAACTCTC AGGGCAGAGG 
TCAGACGTGT GGGCTGGTAG TGTTGGAAAC CACTTTGGCT ATTTTCCAAA AGACTATCTT 
AATATAAATC ATATATATAC TGAAAAAGAA TTGGAGGTAC CTACAGAAGA AACAGATTTT 
GTTTGCTTTG AGACTGGGCT TGATAAATTT GAAAGTTATG ACATAGATGT GTTATTAGGA 
AGCAAATTAT TTATGGAAAT TGAGAATTCA ACAGAAGAAT CAAAAGAAGC AGATCCGTAT 
GACATTTCTG AAACAGAAGA ATCAGTGGAG CATCTTGATT CAGAATCTCT GCTTGAATCA 
GAGGCAGTTC ATTCAGAATC AAGATTGCCT CTTCAATCAG AGTTGATCGA TTCAGAATCA 
GTATTGATTC TCCAACAAGA GTCAGTTGAT TCTGAATCAA AATTGCCTCT TGAATTAGAA 
TCAGTCAATT TAGAATCAGC ATCGACTCAT AAGTCAGTTA ATTCAGAAAC AGAATCGACT 
CATGAATCAG AGTTTGTTTA TTCGAAACCT GCATTGACTC CTGAATCAGA GTCAGTTGAT 
TCAGAATCAA GAACAAAGTC AATTGATTCA GAATCAACAA CACAGTTAAC TAATTCAGAA 
TCAACAACAC AGTCAACTGA TTCAGAATCA AGAACACAGT CAACTGATTC AGAATCAACA 
AAACAGTCAA CTAATTCAGA ATCAAGAACA CAGTCAACTG ATTCAGAATC AATGTTAAAG 
TCAACCGATT CAGAATTAAC CACACAGTCA ACCGATTTAG AATCAACTGA TTCAGAATCA 
ACAGCAGAGT CAACTGATTC AGAATCAACA ACACAGTCGA ATGATTTAGA ATCAACATCA 
CAGTCAACGG ATTCAGAATC AACAGCAGAG TCAAATGATT CAGAATCAGC AACACGGTCA 
AATGATTCAG GATCAACATT ACAGTCAAAT GATTCAGAAT CAGCAACACA GTCAAATGAT 
TCAGGATCAA CATTACAGTC AAATGCTTCA GAATCAACGT CGCAGTCAGC AGATTCAGAA 
TCAACAACAC AGCCAATTGA TTCAGAATCA ACATTGGCTC TTGAATCAGA ATCATTTGAG 
TCAGAATTAA AATCATTACC TCTTGAGTCA GAGTCATTTG ATTCAGAATC AACACTGCCT 
CTTAAATCAG AGTCAGTAGA TTCAGAATCT GTAGAAGCTT CTGAATCAGA CACATTGATT 
CCTAAATCTA CTGAAGATGA CTCTGATAGC TCTCAGCCAA AGACCGTCGT CTCCTCAGAG 
TCAACACTGG AGCCTAAAGA AGCAGCGACA GAAGAGACAG ATACTTTAAC AAGCCAAGAT 
GATGTAGAAA CACAACCATC AGATTTTGAT GCACTAAACA TTGATGATGT TGAACTTTTG 
CACATCCAAC ATGACTCTGA TTCTGAGTCA CAGCTTGAAA CACAAACAGC AGGTTTAGAT 
GTAGATATCG AAGAGCCTGC TAGCCATAAA GATTTACCAC TACAAACTTT AGAGAAAACA 
CTTGAGGATG AGGACAGCTG GTCATCAGCA TTAAATGAAA AAAGTGAATC GAAAGAGACT 
GAATCTTTTC ACGAACACGC CAAAGAATCA CTTTTAGTTC ATGACCTCAA AGCACAAAGA 
GATTTTGAAG AAGAGGAAAA TATTGATGAT GTGGAATTTT CTCATTCTCC TCACAATACG 
AAATCTGCTT CAGATGAGAG TGAAGTCGTC CCATCTGAAC CAGCTCTGGA AACATTAGCA 
TTAAATATAA GTGAAATCAC AGAGAATACA AAGGAAAATG CAGATGATTT TGTTATGAAG 
GCCAGCATGA ACACCAATGA AAATGCACAC AAAAACATTG CAGACGATGC TCGGGATGCA 
GCGATAAACA GAAGCCAACA GCCTACAGAG TCCATCGATG AAGATGAAAC TGAGAACGTT 
TCTGAAAGCC CAGAATCTGC TCCTCTACTT TATAAAACAG ACGAACAGAT CGAAAAGGTG 
AAGAACGAAC TGGAGGATCT GCTTAAAAAC ACACTGGAGA CTGAGGAAGA TAGTCCTGGA 
GGTGAAGAGG ACGAGGCTGA AGAGCTTCTG GAAGACGAAA ACGCTCTTCT TTCATCTAAT 
AGTCCTCACC TCACAGACCA AAATCCTGAA GACAATGAGC AACATGATGA TGATGGTTTG 
GTTGAATCTC AACAAAGCGA AAACACAACA CAAGCAGATG AAACTGAAAC TGAAGAGGTT 
GGTCTTCCTC CTGAAGAGCC TGAATACAGC GATAGCGTAC TGAGACTCAC AATATTGCGA 
GATCACTTGA AGGATGAGGA TACCGAGCGC ATGCAGAAGT ACTTCGGCCT GAAGAACATG 
TTCAGAATCG AAGCCATGTT CTCTGATCTG GATCTTGAGA TGAAGTCTGC TCGGGAGATG 
AAGACGGATA CGGAGGAGAT CGAGCAAACG CTGGACCAGA TCACGGAGGC TTCAGAAAAC 
TCCATTCTCG ATGCCACAGA GGACATACTT AACGACAGAG ACCGGAAAGC TCTGGAGGAT 
GGACTGCAGA AGCAACCGGA GATGTATGAT GTTGAGGCCG CCATGTTGGA CGCTTTTCAG 
GAGATCGTTT TCGCTTTGCG ACAGAAATAT TCGACAGCCA GCGACAGCGT CCCATTGGTC 
GAGGAGGAAC AAAGTGCATA TGAAAGAGAT GACCCCCGAG CTGACGCTGA AGAGGTGAAC 
AATCTGGACA TTACAACTGA AATAACTGAG CCAGCTGAAA CTCCTGAAAT GCACAAGGAG 
CAGAATGAAT CTGAACCGAT TCTGAATCCT GAATTGAACG TGAACCCAGA TTTGTCCCAC 
AATAAAGATG TGGATCTTGA GGAGGACGGA GGCCATTTCA ACAGGAATAA AGACGCTCAA 
ATCAGTTTCG AAGACGCTGA GGAGATCCAA AAGGGACCTC ATGCTATTTT GGAGAAGCCA 
GTGGACATTG GCTTTCACTT TGAGATGGAT CCGTCTTCAG GTTCGTTGGA GTCTCCGAGT 
GTCTCCGATT TCCATGATGC AGAAACTGGC AGTGATTCCT CCGACTCATC GACTTCAGAT 
GAACTTCAGG ATTTGGTGGT GCTTGTCAAA GAAAATCTGG GAGTTTATGG AGAGATTCTG 
ATCACTGCGC TTCCAGAGGA ATGGCGACCG GGTCCCAATT TTCACGGAGT CCCCTGGGAG 
CCAGTGGTGG TCACAGTGGC CATCGGGGCC CTCACTATAC TGATGTTCTT CTGGAGGACG 
GTGCTCGCTA TTAAAGGCAG AAGCTATCAG TTAACTGAAA AGCAGCTTAG AGACAAAATC 
CAGCAGCTTC TCAATGAGAA ATCTGATGCT GCGAACAAGA TCACAGAATT AAATGACATG 
ATAAAAGAAA GAGAACAACG CCTGCAAAAC TCAGAGAAGT CGCTGAGTTC CAGCCAACAT 
GAAATAAAAG GGCTGAAGAA TCATCACCAG AAAATGCAGA GTCAGTGGGA GCAGATGTCT 
GGGAGTATTT CGCAGCTCAA TCAGACAATT GTGGACACAC AGGAAGAAAA CGCAAACCTT 
AATGAAAAGA TTTCCAAGAT GCACCAGAGA ATTGAGAAAT ACCAGAAAAC ACTGAAGAAT 
TACGATGATG AGCGTGCAAA GGTTCATGTC CTTATGGATG AAGCTAAACT CCGAGAAGAC 
GCTCTTAAGG CTCAGGTTCT GTCTTTTGAG AAGGAAAATG GTGCTTTAAA AGAGCAGAAG 
AAATCTCTCC TTGGCGACGC TAAAGACTGG CAGGAGAAGC ACAAGAAGCT GAGCGAGGAG 
ATCCGAGTTT ATCACAAATC CCAGAAAGAG CTGGAAGACT CTCTGGTGCA CAAGGAGAAC 
GAGATCGATG TTTTGTCCAG CTGTATCGCC GAGCTCAACC GACTGGGAGG CTGCGACTCT 
GCAGAGCTAC AGAAAGAAGA TGCTAGAATG TCTAACGGGG ATGAATCTGA GAAGAAAATG 
GACACTATGA GACTGCGAAT CAAACAGATG ATGGACGTCT CCAGGATTAA AGCCACTCTC 
AGCGTTGTGG AGGACGAGAG GAATCGCTAT ATGGAAAATC TCCTCGCTGA ACAGAAATCC 
AGACAAGAGC TGGAAGAGCA ATATCAGAAG GTCATGCATG ACCAGATGAA TCTGAACAAT 
GAGAAAACGC ATCTGGAGAA CCAGATGAAG AACCTGCAGC AGAGACTGGA GATCACCACT 
GAACTCTACC AGCAGAAGGA GAACGCACTG CAACAAAAGT TGACTCAGGA GGAGCTGGAG 
AGACGCGAGA AGGAGACGAA GCTGTCGGAG GTGGACAGTA AAGCGCTGCG CTCGGAGGAG 
GAGGTTCGAG CTCTCAAGCA GAAGATAAAG GACATCGAGG AGGAGATGCA GCAGAACGAA 
CGCTCACTCA AATCTGAGGT GGCAGTGCAA GAGAAGAAGG CCCATGAGAA CTGGTTAAAA 
GCACGCGCCT CAGAGAGAAC TCTGGTGGAG GAGAGAAGAG AATCAGCCAC TCTTCGGCAG 
AAGCTTGTGG AATACCGAGA TAAAATATCC GACATGGAAC AAAGTCTGTT TAAGTTGAAC 
TCTGGGCCGC CAGATCGACA CATGCCGCCG CCACAGCGCA GAGGGGATTC GTACGGTCCA 
TCTCCTGTGA GCGGCGGGGC TCCGTCTCCT CCTTTGATGA TCGAGGGTCC AGGACGGCCC 
CCATCAGCAC CTGTAGGGCG ACGCGGTGAG CCGTTCGGTC CACGACCCCC GTCTGATCCT 
CACGGACGCT TCTCTGAGCT TGGACACCCG CTGCCATCCA GACCAGAAAT GTTTCAGCCG 
ATGACATCAT CTCCATGTGC TCATGATGGG CCGATGACGG CACCAGTCGT GGAGCCAGCC 
GAGTCCTCAG ATCAGGCCTC ATCTGAGCCT GTAGAGTCTC TGTCCAAATC TCAGAGTCAG 
GGATCCTTCC TGCCCTCCCC GATCCGAGAT TCTCCGGTTC CTCCACCTAA CGTCCCGCTG 
AAGCCTTACG GTCCCCCAGG GATGCCGCCC AATGGGCCAC CGATGATGAT CCGACCACCC 
AACGGACACC TTCCCATGAT GCCCCCCGAC CCGCGCTTCA GACCTCCACA CATGGACTCT 
TACGGCCCGC CTCCACACAT CGGCCCGTTT GGACCCGTGC CTCCACCTTT TGCTCGAGGG 
CCTCCACTGG GGCCGCCACT CGGACAGCGG GACGTCCCGC CAGATTTCTT TGCACCCCGC 
GGCCTCCCTC CTCGGCCCTT TCCGCCTGGA CCTCTGCCAC CCCCCGGAGC CATGATCCCT 
CCTCCATACA GCGGACACCG TTTTCCAGCA GCCCCTGCAG TACTGTCTCA GAGCTCCAGA 
GATGGAGACG AGGAGCCGCC GCAGACCCAC GGGGAGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160950.1
CDS	152..5773
Translation	MAAPNNPRFYSLFTLLLCSCFGGSCSDRRFSELKRCADEECSMLLGRGKAAKDFTGPDCRFLSFKKGETIYVYYKLSGQRSDVWAGSVGNHFGYFPKDYLNINHIYTEKELEVPTEETDFVCFETGLDKFESYDIDVLLGSKLFMEIENSTEESKEADPYDISETEESVEHLDSESLLESEAVHSESRLPLQSELIDSESVLILQQESVDSESKLPLELESVNLESASTHKSVNSETESTHESEFVYSKPALTPESESVDSESRTKSIDSESTTQLTNSESTTQSTDSESRTQSTDSESTKQSTNSESRTQSTDSESMLKSTDSELTTQSTDLESTDSESTAESTDSESTTQSNDLESTSQSTDSESTAESNDSESATRSNDSGSTLQSNDSESATQSNDSGSTLQSNASESTSQSADSESTTQPIDSESTLALESESFESELKSLPLESESFDSESTLPLKSESVDSESVEASESDTLIPKSTEDDSDSSQPKTVVSSESTLEPKEAATEETDTLTSQDDVETQPSDFDALNIDDVELLHIQHDSDSESQLETQTAGLDVDIEEPASHKDLPLQTLEKTLEDEDSWSSALNEKSESKETESFHEHAKESLLVHDLKAQRDFEEEENIDDVEFSHSPHNTKSASDESEVVPSEPALETLALNISEITENTKENADDFVMKASMNTNENAHKNIADDARDAAINRSQQPTESIDEDETENVSESPESAPLLYKTDEQIEKVKNELEDLLKNTLETEEDSPGGEEDEAEELLEDENALLSSNSPHLTDQNPEDNEQHDDDGLVESQQSENTTQADETETEEVGLPPEEPEYSDSVLRLTILRDHLKDEDTERMQKYFGLKNMFRIEAMFSDLDLEMKSAREMKTDTEEIEQTLDQITEASENSILDATEDILNDRDRKALEDGLQKQPEMYDVEAAMLDAFQEIVFALRQKYSTASDSVPLVEEEQSAYERDDPRADAEEVNNLDITTEITEPAETPEMHKEQNESEPILNPELNVNPDLSHNKDVDLEEDGGHFNRNKDAQISFEDAEEIQKGPHAILEKPVDIGFHFEMDPSSGSLESPSVSDFHDAETGSDSSDSSTSDELQDLVVLVKENLGVYGEILITALPEEWRPGPNFHGVPWEPVVVTVAIGALTILMFFWRTVLAIKGRSYQLTEKQLRDKIQQLLNEKSDAANKITELNDMIKEREQRLQNSEKSLSSSQHEIKGLKNHHQKMQSQWEQMSGSISQLNQTIVDTQEENANLNEKISKMHQRIEKYQKTLKNYDDERAKVHVLMDEAKLREDALKAQVLSFEKENGALKEQKKSLLGDAKDWQEKHKKLSEEIRVYHKSQKELEDSLVHKENEIDVLSSCIAELNRLGGCDSAELQKEDARMSNGDESEKKMDTMRLRIKQMMDVSRIKATLSVVEDERNRYMENLLAEQKSRQELEEQYQKVMHDQMNLNNEKTHLENQMKNLQQRLEITTELYQQKENALQQKLTQEELERREKETKLSEVDSKALRSEEEVRALKQKIKDIEEEMQQNERSLKSEVAVQEKKAHENWLKARASERTLVEERRESATLRQKLVEYRDKISDMEQSLFKLNSGPPDRHMPPPQRRGDSYGPSPVSGGAPSPPLMIEGPGRPPSAPVGRRGEPFGPRPPSDPHGRFSELGHPLPSRPEMFQPMTSSPCAHDGPMTAPVVEPAESSDQASSEPVESLSKSQSQGSFLPSPIRDSPVPPPNVPLKPYGPPGMPPNGPPMMIRPPNGHLPMMPPDPRFRPPHMDSYGPPPHIGPFGPVPPPFARGPPLGPPLGQRDVPPDFFAPRGLPPRPFPPGPLPPPGAMIPPPYSGHRFPAAPAVLSQSSRDGDEEPPQTHGEP

Target ORF information:

RefSeq Version	XM_005160893.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio melanoma inhibitory activity family, member 3 (mia3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160893.4

ORF Insert Sequence:

ATGGCTGCAC CGAATAATCC CCGGTTTTAC TCACTTTTTA CCCTCCTCCT GTGCTCGTGC 
TTCGGCGGGA GCTGCTCGGA CCGGCGGTTC TCGGAGCTGA AGCGCTGCGC GGATGAAGAA 
TGCAGCATGC TTCTCGGCAG AGGAAAGGCC GCAAAGGATT TCACGGGGCC GGACTGTCGG 
TTTCTCTCTT TTAAGAAAGG AGAGACGATA TATGTATATT ATAAACTCTC AGGGCAGAGG 
TCAGACGTGT GGGCTGGTAG TGTTGGAAAC CACTTTGGCT ATTTTCCAAA AGACTATCTT 
AATATAAATC ATATATATAC TGAAAAAGAA TTGGAGGTAC CTACAGAAGA AACAGATTTT 
GTTTGCTTTG AGACTGGGCT TGATAAATTT GAAAGTTATG ACATAGATGT GTTATTAGGA 
AGCAAATTAT TTATGGAAAT TGAGAATTCA ACAGAAGAAT CAAAAGAAGC AGATCCGTAT 
GACATTTCTG AAACAGAAGA ATCAGTGGAG CATCTTGATT CAGAATCTCT GCTTGAATCA 
GAGGCAGTTC ATTCAGAATC AAGATTGCCT CTTCAATCAG AGTTGATCGA TTCAGAATCA 
GTATTGATTC TCCAACAAGA GTCAGTTGAT TCTGAATCAA AATTGCCTCT TGAATTAGAA 
TCAGTCAATT TAGAATCAGC ATCGACTCAT AAGTCAGTTA ATTCAGAAAC AGAATCGACT 
CATGAATCAG AGTTTGTTTA TTCGAAACCT GCATTGACTC CTGAATCAGA GTCAGTTGAT 
TCAGAATCAA GAACAAAGTC AATTGATTCA GAATCAACAA CACAGTTAAC TAATTCAGAA 
TCAACAACAC AGTCAACTGA TTCAGAATCA AGAACACAGT CAACTGATTC AGAATCAACA 
AAACAGTCAA CTAATTCAGA ATCAAGAACA CAGTCAACTG ATTCAGAATC AATGTTAAAG 
TCAACCGATT CAGAATTAAC CACACAGTCA ACCGATTTAG AATCAACTGA TTCAGAATCA 
ACAGCAGAGT CAACTGATTC AGAATCAACA ACACAGTCGA ATGATTTAGA ATCAACATCA 
CAGTCAACGG ATTCAGAATC AACAGCAGAG TCAAATGATT CAGAATCAGC AACACGGTCA 
AATGATTCAG GATCAACATT ACAGTCAAAT GATTCAGAAT CAGCAACACA GTCAAATGAT 
TCAGGATCAA CATTACAGTC AAATGCTTCA GAATCAACGT CGCAGTCAGC AGATTCAGAA 
TCAACAACAC AGCCAATTGA TTCAGAATCA ACATTGGCTC TTGAATCAGA ATCATTTGAG 
TCAGAATTAA AATCATTACC TCTTGAGTCA GAGTCATTTG ATTCAGAATC AACACTGCCT 
CTTAAATCAG AGTCAGTAGA TTCAGAATCT GTAGAAGCTT CTGAATCAGA CACATTGATT 
CCTAAATCTA CTGAAGATGA CTCTGATAGC TCTCAGCCAA AGACCGTCGT CTCCTCAGAG 
TCAACACTGG AGCCTAAAGA AGCAGCGACA GAAGAGACAG ATACTTTAAC AAGCCAAGAT 
GATGTAGAAA CACAACCATC AGATTTTGAT GCACTAAACA TTGATGATGT TGAACTTTTG 
CACATCCAAC ATGACTCTGA TTCTGAGTCA CAGCTTGAAA CACAAACAGC AGGTTTAGAT 
GTAGATATCG AAGAGCCTGC TAGCCATAAA GATTTACCAC TACAAACTTT AGAGAAAACA 
CTTGAGGATG AGGACAGCTG GTCATCAGCA TTAAATGAAA AAAGTGAATC GAAAGAGACT 
GAATCTTTTC ACGAACACGC CAAAGAATCA CTTTTAGTTC ATGACCTCAA AGCACAAAGA 
GATTTTGAAG AAGAGGAAAA TATTGATGAT GTGGAATTTT CTCATTCTCC TCACAATACG 
AAATCTGCTT CAGATGAGAG TGAAGTCGTC CCATCTGAAC CAGCTCTGGA AACATTAGCA 
TTAAATATAA GTGAAATCAC AGAGAATACA AAGGAAAATG CAGATGATTT TGTTATGAAG 
GCCAGCATGA ACACCAATGA AAATGCACAC AAAAACATTG CAGACGATGC TCGGGATGCA 
GCGATAAACA GAAGCCAACA GCCTACAGAG TCCATCGATG AAGATGAAAC TGAGAACGTT 
TCTGAAAGCC CAGAATCTGC TCCTCTACTT TATAAAACAG ACGAACAGAT CGAAAAGGTG 
AAGAACGAAC TGGAGGATCT GCTTAAAAAC ACACTGGAGA CTGAGGAAGA TAGTCCTGGA 
GGTGAAGAGG ACGAGGCTGA AGAGCTTCTG GAAGACGAAA ACGCTCTTCT TTCATCTAAT 
AGTCCTCACC TCACAGACCA AAATCCTGAA GACAATGAGC AACATGATGA TGATGGTTTG 
GTTGAATCTC AACAAAGCGA AAACACAACA CAAGCAGATG AAACTGAAAC TGAAGAGGTT 
GGTCTTCCTC CTGAAGAGCC TGAATACAGC GATAGCGTAC TGAGACTCAC AATATTGCGA 
GATCACTTGA AGGATGAGGA TACCGAGCGC ATGCAGAAGT ACTTCGGCCT GAAGAACATG 
TTCAGAATCG AAGCCATGTT CTCTGATCTG GATCTTGAGA TGAAGTCTGC TCGGGAGATG 
AAGACGGATA CGGAGGAGAT CGAGCAAACG CTGGACCAGA TCACGGAGGC TTCAGAAAAC 
TCCATTCTCG ATGCCACAGA GGACATACTT AACGACAGAG ACCGGAAAGC TCTGGAGGAT 
GGACTGCAGA AGCAACCGGA GATGTATGAT GTTGAGGCCG CCATGTTGGA CGCTTTTCAG 
GAGATCGTTT TCGCTTTGCG ACAGAAATAT TCGACAGCCA GCGACAGCGT CCCATTGGTC 
GAGGAGGAAC AAAGTGCATA TGAAAGAGAT GACCCCCGAG CTGACGCTGA AGAGGTGAAC 
AATCTGGACA TTACAACTGA AATAACTGAG CCAGCTGAAA CTCCTGAAAT GCACAAGGAG 
CAGAATGAAT CTGAACCGAT TCTGAATCCT GAATTGAACG TGAACCCAGA TTTGTCCCAC 
AATAAAGATG TGGATCTTGA GGAGGACGGA GGCCATTTCA ACAGGAATAA AGACGCTCAA 
ATCAGTTTCG AAGACGCTGA GGAGATCCAA AAGGGACCTC ATGCTATTTT GGAGAAGCCA 
GTGGACATTG GCTTTCACTT TGAGATGGAT CCGTCTTCAG GTTCGTTGGA GTCTCCGAGT 
GTCTCCGATT TCCATGATGC AGAAACTGGC AGTGATTCCT CCGACTCATC GACTTCAGAT 
GAACTTCAGG ATTTGGTGGT GCTTGTCAAA GAAAATCTGG GAGTTTATGG AGAGATTCTG 
ATCACTGCGC TTCCAGAGGA ATGGCGACCG GGTCCCAATT TTCACGGAGT CCCCTGGGAG 
CCAGTGGTGG TCACAGTGGC CATCGGGGCC CTCACTATAC TGATGTTCTT CTGGAGGACG 
GTGCTCGCTA TTAAAGGCAG AAGCTATCAG TTAACTGAAA AGCAGCTTAG AGACAAAATC 
CAGCAGCTTC TCAATGAGAA ATCTGATGCT GCGAACAAGA TCACAGAATT AAATGACATG 
ATAAAAGAAA GAGAACAACG CCTGCAAAAC TCAGAGAAGT CGCTGAGTTC CAGCCAACAT 
GAAATAAAAG GGCTGAAGAA TCATCACCAG AAAATGCAGA GTCAGTGGGA GCAGATGTCT 
GGGAGTATTT CGCAGCTCAA TCAGACAATT GTGGACACAC AGGAAGAAAA CGCAAACCTT 
AATGAAAAGA TTTCCAAGAT GCACCAGAGA ATTGAGAAAT ACCAGAAAAC ACTGAAGAAT 
TACGATGATG AGCGTGCAAA GGTTCATGTC CTTATGGATG AAGCTAAACT CCGAGAAGAC 
GCTCTTAAGG CTCAGGTTCT GTCTTTTGAG AAGGAAAATG GTGCTTTAAA AGAGCAGAAG 
AAATCTCTCC TTGGCGACGC TAAAGACTGG CAGGAGAAGC ACAAGAAGCT GAGCGAGGAG 
ATCCGAGTTT ATCACAAATC CCAGAAAGAG CTGGAAGACT CTCTGGTGCA CAAGGAGAAC 
GAGATCGATG TTTTGTCCAG CTGTATCGCC GAGCTCAACC GACTGGGAGG CTGCGACTCT 
GCAGAGCTAC AGAAAGAAGA TGCTAGAATG TCTAACGGGG ATGAATCTGA GAAGAAAATG 
GACACTATGA GACTGCGAAT CAAACAGATG ATGGACGTCT CCAGGATTAA AGCCACTCTC 
AGCGTTGTGG AGGACGAGAG GAATCGCTAT ATGGAAAATC TCCTCGCTGA ACAGAAATCC 
AGACAAGAGC TGGAAGAGCA ATATCAGAAG GTCATGCATG ACCAGATGAA TCTGAACAAT 
GAGAAAACGC ATCTGGAGAA CCAGATGAAG AACCTGCAGC AGAGACTGGA GATCACCACT 
GAACTCTACC AGCAGAAGGA GAACGCACTG CAACAAAAGT TGACTCAGGA GGAGCTGGAG 
AGACGCGAGA AGGAGACGAA GCTGTCGGAG GTGGACAGTA AAGCGCTGCG CTCGGAGGAG 
GAGGTTCGAG CTCTCAAGCA GAAGATAAAG GACATCGAGG AGGAGATGCA GCAGAACGAA 
CGCTCACTCA AATCTGAGGT GGCAGTGCAA GAGAAGAAGG CCCATGAGAA CTGGTTAAAA 
GCACGCGCCT CAGAGAGAAC TCTGGTGGAG GAGAGAAGAG AATCAGCCAC TCTTCGGCAG 
AAGCTTGTGG AATACCGAGA TAAAATATCC GACATGGAAC AAAGTCTGTT TAAGTTGAAC 
TCTGGGCCGC CAGATCGACA CATGCCGCCG CCACAGCGCA GAGGGGATTC GTACGGTCCA 
TCTCCTGTGA GCGGCGGGGC TCCGTCTCCT CCTTTGATGA TCGAGGGTCC AGGACGGCCC 
CCATCAGCAC CTGTAGGGCG ACGCGGTGAG CCGTTCGGTC CACGACCCCC GTCTGATCCT 
CACGGACGCT TCTCTGAGCT TGGACACCCG CTGCCATCCA GACCAGAAAT GTTTCAGCCG 
ATGACATCAT CTCCATGTGC TCATGATGGG CCGATGACGG CACCAGTCGT GGAGCCAGCC 
GAGTCCTCAG ATCAGGCCTC ATCTGAGCCT GTAGAGTCTC TGTCCAAATC TCAGAGTCAG 
GGATCCTTCC TGCCCTCCCC GATCCGAGAT TCTCCGGTTC CTCCACCTAA CGTCCCGCTG 
AAGCCTTACG GTCCCCCAGG GATGCCGCCC AATGGGCCAC CGATGATGAT CCGACCACCC 
AACGGACACC TTCCCATGAT GCCCCCCGAC CCGCGCTTCA GACCTCCACA CATGGACTCT 
TACGGCCCGC CTCCACACAT CGGCCCGTTT GGACCCGTGC CTCCACCTTT TGCTCGAGGG 
CCTCCACTGG GGCCGCCACT CGGACAGCGG GACGTCCCGC CAGATTTCTT TGCACCCCGC 
GGCCTCCCTC CTCGGCCCTT TCCGCCTGGA CCTCTGCCAC CCCCCGGAGC CATGATCCCT 
CCTCCATACA GCGGACACCG TTTTCCAGCA GCCCCTGCAG TACTGTCTCA GAGCTCCAGA 
GATGGAGACG AGGAGCCGCC GCAGACCCAC GGGGAGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mia3 ( XM_005160893.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160950.1 Danio rerio melanoma inhibitory activity family, member 3 (mia3), transcript variant X2, mRNA.

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CloneID	ODa25318
Clone ID Related Accession (Same CDS sequence)	XM_005160893.4 , XM_005160893.3
Accession Version	XM_005160893.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5622bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	melanoma inhibitory activity protein 3 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160893.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAC CGAATAATCC CCGGTTTTAC TCACTTTTTA CCCTCCTCCT GTGCTCGTGC 
TTCGGCGGGA GCTGCTCGGA CCGGCGGTTC TCGGAGCTGA AGCGCTGCGC GGATGAAGAA 
TGCAGCATGC TTCTCGGCAG AGGAAAGGCC GCAAAGGATT TCACGGGGCC GGACTGTCGG 
TTTCTCTCTT TTAAGAAAGG AGAGACGATA TATGTATATT ATAAACTCTC AGGGCAGAGG 
TCAGACGTGT GGGCTGGTAG TGTTGGAAAC CACTTTGGCT ATTTTCCAAA AGACTATCTT 
AATATAAATC ATATATATAC TGAAAAAGAA TTGGAGGTAC CTACAGAAGA AACAGATTTT 
GTTTGCTTTG AGACTGGGCT TGATAAATTT GAAAGTTATG ACATAGATGT GTTATTAGGA 
AGCAAATTAT TTATGGAAAT TGAGAATTCA ACAGAAGAAT CAAAAGAAGC AGATCCGTAT 
GACATTTCTG AAACAGAAGA ATCAGTGGAG CATCTTGATT CAGAATCTCT GCTTGAATCA 
GAGGCAGTTC ATTCAGAATC AAGATTGCCT CTTCAATCAG AGTTGATCGA TTCAGAATCA 
GTATTGATTC TCCAACAAGA GTCAGTTGAT TCTGAATCAA AATTGCCTCT TGAATTAGAA 
TCAGTCAATT TAGAATCAGC ATCGACTCAT AAGTCAGTTA ATTCAGAAAC AGAATCGACT 
CATGAATCAG AGTTTGTTTA TTCGAAACCT GCATTGACTC CTGAATCAGA GTCAGTTGAT 
TCAGAATCAA GAACAAAGTC AATTGATTCA GAATCAACAA CACAGTTAAC TAATTCAGAA 
TCAACAACAC AGTCAACTGA TTCAGAATCA AGAACACAGT CAACTGATTC AGAATCAACA 
AAACAGTCAA CTAATTCAGA ATCAAGAACA CAGTCAACTG ATTCAGAATC AATGTTAAAG 
TCAACCGATT CAGAATTAAC CACACAGTCA ACCGATTTAG AATCAACTGA TTCAGAATCA 
ACAGCAGAGT CAACTGATTC AGAATCAACA ACACAGTCGA ATGATTTAGA ATCAACATCA 
CAGTCAACGG ATTCAGAATC AACAGCAGAG TCAAATGATT CAGAATCAGC AACACGGTCA 
AATGATTCAG GATCAACATT ACAGTCAAAT GATTCAGAAT CAGCAACACA GTCAAATGAT 
TCAGGATCAA CATTACAGTC AAATGCTTCA GAATCAACGT CGCAGTCAGC AGATTCAGAA 
TCAACAACAC AGCCAATTGA TTCAGAATCA ACATTGGCTC TTGAATCAGA ATCATTTGAG 
TCAGAATTAA AATCATTACC TCTTGAGTCA GAGTCATTTG ATTCAGAATC AACACTGCCT 
CTTAAATCAG AGTCAGTAGA TTCAGAATCT GTAGAAGCTT CTGAATCAGA CACATTGATT 
CCTAAATCTA CTGAAGATGA CTCTGATAGC TCTCAGCCAA AGACCGTCGT CTCCTCAGAG 
TCAACACTGG AGCCTAAAGA AGCAGCGACA GAAGAGACAG ATACTTTAAC AAGCCAAGAT 
GATGTAGAAA CACAACCATC AGATTTTGAT GCACTAAACA TTGATGATGT TGAACTTTTG 
CACATCCAAC ATGACTCTGA TTCTGAGTCA CAGCTTGAAA CACAAACAGC AGGTTTAGAT 
GTAGATATCG AAGAGCCTGC TAGCCATAAA GATTTACCAC TACAAACTTT AGAGAAAACA 
CTTGAGGATG AGGACAGCTG GTCATCAGCA TTAAATGAAA AAAGTGAATC GAAAGAGACT 
GAATCTTTTC ACGAACACGC CAAAGAATCA CTTTTAGTTC ATGACCTCAA AGCACAAAGA 
GATTTTGAAG AAGAGGAAAA TATTGATGAT GTGGAATTTT CTCATTCTCC TCACAATACG 
AAATCTGCTT CAGATGAGAG TGAAGTCGTC CCATCTGAAC CAGCTCTGGA AACATTAGCA 
TTAAATATAA GTGAAATCAC AGAGAATACA AAGGAAAATG CAGATGATTT TGTTATGAAG 
GCCAGCATGA ACACCAATGA AAATGCACAC AAAAACATTG CAGACGATGC TCGGGATGCA 
GCGATAAACA GAAGCCAACA GCCTACAGAG TCCATCGATG AAGATGAAAC TGAGAACGTT 
TCTGAAAGCC CAGAATCTGC TCCTCTACTT TATAAAACAG ACGAACAGAT CGAAAAGGTG 
AAGAACGAAC TGGAGGATCT GCTTAAAAAC ACACTGGAGA CTGAGGAAGA TAGTCCTGGA 
GGTGAAGAGG ACGAGGCTGA AGAGCTTCTG GAAGACGAAA ACGCTCTTCT TTCATCTAAT 
AGTCCTCACC TCACAGACCA AAATCCTGAA GACAATGAGC AACATGATGA TGATGGTTTG 
GTTGAATCTC AACAAAGCGA AAACACAACA CAAGCAGATG AAACTGAAAC TGAAGAGGTT 
GGTCTTCCTC CTGAAGAGCC TGAATACAGC GATAGCGTAC TGAGACTCAC AATATTGCGA 
GATCACTTGA AGGATGAGGA TACCGAGCGC ATGCAGAAGT ACTTCGGCCT GAAGAACATG 
TTCAGAATCG AAGCCATGTT CTCTGATCTG GATCTTGAGA TGAAGTCTGC TCGGGAGATG 
AAGACGGATA CGGAGGAGAT CGAGCAAACG CTGGACCAGA TCACGGAGGC TTCAGAAAAC 
TCCATTCTCG ATGCCACAGA GGACATACTT AACGACAGAG ACCGGAAAGC TCTGGAGGAT 
GGACTGCAGA AGCAACCGGA GATGTATGAT GTTGAGGCCG CCATGTTGGA CGCTTTTCAG 
GAGATCGTTT TCGCTTTGCG ACAGAAATAT TCGACAGCCA GCGACAGCGT CCCATTGGTC 
GAGGAGGAAC AAAGTGCATA TGAAAGAGAT GACCCCCGAG CTGACGCTGA AGAGGTGAAC 
AATCTGGACA TTACAACTGA AATAACTGAG CCAGCTGAAA CTCCTGAAAT GCACAAGGAG 
CAGAATGAAT CTGAACCGAT TCTGAATCCT GAATTGAACG TGAACCCAGA TTTGTCCCAC 
AATAAAGATG TGGATCTTGA GGAGGACGGA GGCCATTTCA ACAGGAATAA AGACGCTCAA 
ATCAGTTTCG AAGACGCTGA GGAGATCCAA AAGGGACCTC ATGCTATTTT GGAGAAGCCA 
GTGGACATTG GCTTTCACTT TGAGATGGAT CCGTCTTCAG GTTCGTTGGA GTCTCCGAGT 
GTCTCCGATT TCCATGATGC AGAAACTGGC AGTGATTCCT CCGACTCATC GACTTCAGAT 
GAACTTCAGG ATTTGGTGGT GCTTGTCAAA GAAAATCTGG GAGTTTATGG AGAGATTCTG 
ATCACTGCGC TTCCAGAGGA ATGGCGACCG GGTCCCAATT TTCACGGAGT CCCCTGGGAG 
CCAGTGGTGG TCACAGTGGC CATCGGGGCC CTCACTATAC TGATGTTCTT CTGGAGGACG 
GTGCTCGCTA TTAAAGGCAG AAGCTATCAG TTAACTGAAA AGCAGCTTAG AGACAAAATC 
CAGCAGCTTC TCAATGAGAA ATCTGATGCT GCGAACAAGA TCACAGAATT AAATGACATG 
ATAAAAGAAA GAGAACAACG CCTGCAAAAC TCAGAGAAGT CGCTGAGTTC CAGCCAACAT 
GAAATAAAAG GGCTGAAGAA TCATCACCAG AAAATGCAGA GTCAGTGGGA GCAGATGTCT 
GGGAGTATTT CGCAGCTCAA TCAGACAATT GTGGACACAC AGGAAGAAAA CGCAAACCTT 
AATGAAAAGA TTTCCAAGAT GCACCAGAGA ATTGAGAAAT ACCAGAAAAC ACTGAAGAAT 
TACGATGATG AGCGTGCAAA GGTTCATGTC CTTATGGATG AAGCTAAACT CCGAGAAGAC 
GCTCTTAAGG CTCAGGTTCT GTCTTTTGAG AAGGAAAATG GTGCTTTAAA AGAGCAGAAG 
AAATCTCTCC TTGGCGACGC TAAAGACTGG CAGGAGAAGC ACAAGAAGCT GAGCGAGGAG 
ATCCGAGTTT ATCACAAATC CCAGAAAGAG CTGGAAGACT CTCTGGTGCA CAAGGAGAAC 
GAGATCGATG TTTTGTCCAG CTGTATCGCC GAGCTCAACC GACTGGGAGG CTGCGACTCT 
GCAGAGCTAC AGAAAGAAGA TGCTAGAATG TCTAACGGGG ATGAATCTGA GAAGAAAATG 
GACACTATGA GACTGCGAAT CAAACAGATG ATGGACGTCT CCAGGATTAA AGCCACTCTC 
AGCGTTGTGG AGGACGAGAG GAATCGCTAT ATGGAAAATC TCCTCGCTGA ACAGAAATCC 
AGACAAGAGC TGGAAGAGCA ATATCAGAAG GTCATGCATG ACCAGATGAA TCTGAACAAT 
GAGAAAACGC ATCTGGAGAA CCAGATGAAG AACCTGCAGC AGAGACTGGA GATCACCACT 
GAACTCTACC AGCAGAAGGA GAACGCACTG CAACAAAAGT TGACTCAGGA GGAGCTGGAG 
AGACGCGAGA AGGAGACGAA GCTGTCGGAG GTGGACAGTA AAGCGCTGCG CTCGGAGGAG 
GAGGTTCGAG CTCTCAAGCA GAAGATAAAG GACATCGAGG AGGAGATGCA GCAGAACGAA 
CGCTCACTCA AATCTGAGGT GGCAGTGCAA GAGAAGAAGG CCCATGAGAA CTGGTTAAAA 
GCACGCGCCT CAGAGAGAAC TCTGGTGGAG GAGAGAAGAG AATCAGCCAC TCTTCGGCAG 
AAGCTTGTGG AATACCGAGA TAAAATATCC GACATGGAAC AAAGTCTGTT TAAGTTGAAC 
TCTGGGCCGC CAGATCGACA CATGCCGCCG CCACAGCGCA GAGGGGATTC GTACGGTCCA 
TCTCCTGTGA GCGGCGGGGC TCCGTCTCCT CCTTTGATGA TCGAGGGTCC AGGACGGCCC 
CCATCAGCAC CTGTAGGGCG ACGCGGTGAG CCGTTCGGTC CACGACCCCC GTCTGATCCT 
CACGGACGCT TCTCTGAGCT TGGACACCCG CTGCCATCCA GACCAGAAAT GTTTCAGCCG 
ATGACATCAT CTCCATGTGC TCATGATGGG CCGATGACGG CACCAGTCGT GGAGCCAGCC 
GAGTCCTCAG ATCAGGCCTC ATCTGAGCCT GTAGAGTCTC TGTCCAAATC TCAGAGTCAG 
GGATCCTTCC TGCCCTCCCC GATCCGAGAT TCTCCGGTTC CTCCACCTAA CGTCCCGCTG 
AAGCCTTACG GTCCCCCAGG GATGCCGCCC AATGGGCCAC CGATGATGAT CCGACCACCC 
AACGGACACC TTCCCATGAT GCCCCCCGAC CCGCGCTTCA GACCTCCACA CATGGACTCT 
TACGGCCCGC CTCCACACAT CGGCCCGTTT GGACCCGTGC CTCCACCTTT TGCTCGAGGG 
CCTCCACTGG GGCCGCCACT CGGACAGCGG GACGTCCCGC CAGATTTCTT TGCACCCCGC 
GGCCTCCCTC CTCGGCCCTT TCCGCCTGGA CCTCTGCCAC CCCCCGGAGC CATGATCCCT 
CCTCCATACA GCGGACACCG TTTTCCAGCA GCCCCTGCAG TACTGTCTCA GAGCTCCAGA 
GATGGAGACG AGGAGCCGCC GCAGACCCAC GGGGAGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160950.1
CDS	172..5793
Translation	MAAPNNPRFYSLFTLLLCSCFGGSCSDRRFSELKRCADEECSMLLGRGKAAKDFTGPDCRFLSFKKGETIYVYYKLSGQRSDVWAGSVGNHFGYFPKDYLNINHIYTEKELEVPTEETDFVCFETGLDKFESYDIDVLLGSKLFMEIENSTEESKEADPYDISETEESVEHLDSESLLESEAVHSESRLPLQSELIDSESVLILQQESVDSESKLPLELESVNLESASTHKSVNSETESTHESEFVYSKPALTPESESVDSESRTKSIDSESTTQLTNSESTTQSTDSESRTQSTDSESTKQSTNSESRTQSTDSESMLKSTDSELTTQSTDLESTDSESTAESTDSESTTQSNDLESTSQSTDSESTAESNDSESATRSNDSGSTLQSNDSESATQSNDSGSTLQSNASESTSQSADSESTTQPIDSESTLALESESFESELKSLPLESESFDSESTLPLKSESVDSESVEASESDTLIPKSTEDDSDSSQPKTVVSSESTLEPKEAATEETDTLTSQDDVETQPSDFDALNIDDVELLHIQHDSDSESQLETQTAGLDVDIEEPASHKDLPLQTLEKTLEDEDSWSSALNEKSESKETESFHEHAKESLLVHDLKAQRDFEEEENIDDVEFSHSPHNTKSASDESEVVPSEPALETLALNISEITENTKENADDFVMKASMNTNENAHKNIADDARDAAINRSQQPTESIDEDETENVSESPESAPLLYKTDEQIEKVKNELEDLLKNTLETEEDSPGGEEDEAEELLEDENALLSSNSPHLTDQNPEDNEQHDDDGLVESQQSENTTQADETETEEVGLPPEEPEYSDSVLRLTILRDHLKDEDTERMQKYFGLKNMFRIEAMFSDLDLEMKSAREMKTDTEEIEQTLDQITEASENSILDATEDILNDRDRKALEDGLQKQPEMYDVEAAMLDAFQEIVFALRQKYSTASDSVPLVEEEQSAYERDDPRADAEEVNNLDITTEITEPAETPEMHKEQNESEPILNPELNVNPDLSHNKDVDLEEDGGHFNRNKDAQISFEDAEEIQKGPHAILEKPVDIGFHFEMDPSSGSLESPSVSDFHDAETGSDSSDSSTSDELQDLVVLVKENLGVYGEILITALPEEWRPGPNFHGVPWEPVVVTVAIGALTILMFFWRTVLAIKGRSYQLTEKQLRDKIQQLLNEKSDAANKITELNDMIKEREQRLQNSEKSLSSSQHEIKGLKNHHQKMQSQWEQMSGSISQLNQTIVDTQEENANLNEKISKMHQRIEKYQKTLKNYDDERAKVHVLMDEAKLREDALKAQVLSFEKENGALKEQKKSLLGDAKDWQEKHKKLSEEIRVYHKSQKELEDSLVHKENEIDVLSSCIAELNRLGGCDSAELQKEDARMSNGDESEKKMDTMRLRIKQMMDVSRIKATLSVVEDERNRYMENLLAEQKSRQELEEQYQKVMHDQMNLNNEKTHLENQMKNLQQRLEITTELYQQKENALQQKLTQEELERREKETKLSEVDSKALRSEEEVRALKQKIKDIEEEMQQNERSLKSEVAVQEKKAHENWLKARASERTLVEERRESATLRQKLVEYRDKISDMEQSLFKLNSGPPDRHMPPPQRRGDSYGPSPVSGGAPSPPLMIEGPGRPPSAPVGRRGEPFGPRPPSDPHGRFSELGHPLPSRPEMFQPMTSSPCAHDGPMTAPVVEPAESSDQASSEPVESLSKSQSQGSFLPSPIRDSPVPPPNVPLKPYGPPGMPPNGPPMMIRPPNGHLPMMPPDPRFRPPHMDSYGPPPHIGPFGPVPPPFARGPPLGPPLGQRDVPPDFFAPRGLPPRPFPPGPLPPPGAMIPPPYSGHRFPAAPAVLSQSSRDGDEEPPQTHGEP

Target ORF information:

RefSeq Version	XM_005160893.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio melanoma inhibitory activity family, member 3 (mia3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160893.3

ORF Insert Sequence:

ATGGCTGCAC CGAATAATCC CCGGTTTTAC TCACTTTTTA CCCTCCTCCT GTGCTCGTGC 
TTCGGCGGGA GCTGCTCGGA CCGGCGGTTC TCGGAGCTGA AGCGCTGCGC GGATGAAGAA 
TGCAGCATGC TTCTCGGCAG AGGAAAGGCC GCAAAGGATT TCACGGGGCC GGACTGTCGG 
TTTCTCTCTT TTAAGAAAGG AGAGACGATA TATGTATATT ATAAACTCTC AGGGCAGAGG 
TCAGACGTGT GGGCTGGTAG TGTTGGAAAC CACTTTGGCT ATTTTCCAAA AGACTATCTT 
AATATAAATC ATATATATAC TGAAAAAGAA TTGGAGGTAC CTACAGAAGA AACAGATTTT 
GTTTGCTTTG AGACTGGGCT TGATAAATTT GAAAGTTATG ACATAGATGT GTTATTAGGA 
AGCAAATTAT TTATGGAAAT TGAGAATTCA ACAGAAGAAT CAAAAGAAGC AGATCCGTAT 
GACATTTCTG AAACAGAAGA ATCAGTGGAG CATCTTGATT CAGAATCTCT GCTTGAATCA 
GAGGCAGTTC ATTCAGAATC AAGATTGCCT CTTCAATCAG AGTTGATCGA TTCAGAATCA 
GTATTGATTC TCCAACAAGA GTCAGTTGAT TCTGAATCAA AATTGCCTCT TGAATTAGAA 
TCAGTCAATT TAGAATCAGC ATCGACTCAT AAGTCAGTTA ATTCAGAAAC AGAATCGACT 
CATGAATCAG AGTTTGTTTA TTCGAAACCT GCATTGACTC CTGAATCAGA GTCAGTTGAT 
TCAGAATCAA GAACAAAGTC AATTGATTCA GAATCAACAA CACAGTTAAC TAATTCAGAA 
TCAACAACAC AGTCAACTGA TTCAGAATCA AGAACACAGT CAACTGATTC AGAATCAACA 
AAACAGTCAA CTAATTCAGA ATCAAGAACA CAGTCAACTG ATTCAGAATC AATGTTAAAG 
TCAACCGATT CAGAATTAAC CACACAGTCA ACCGATTTAG AATCAACTGA TTCAGAATCA 
ACAGCAGAGT CAACTGATTC AGAATCAACA ACACAGTCGA ATGATTTAGA ATCAACATCA 
CAGTCAACGG ATTCAGAATC AACAGCAGAG TCAAATGATT CAGAATCAGC AACACGGTCA 
AATGATTCAG GATCAACATT ACAGTCAAAT GATTCAGAAT CAGCAACACA GTCAAATGAT 
TCAGGATCAA CATTACAGTC AAATGCTTCA GAATCAACGT CGCAGTCAGC AGATTCAGAA 
TCAACAACAC AGCCAATTGA TTCAGAATCA ACATTGGCTC TTGAATCAGA ATCATTTGAG 
TCAGAATTAA AATCATTACC TCTTGAGTCA GAGTCATTTG ATTCAGAATC AACACTGCCT 
CTTAAATCAG AGTCAGTAGA TTCAGAATCT GTAGAAGCTT CTGAATCAGA CACATTGATT 
CCTAAATCTA CTGAAGATGA CTCTGATAGC TCTCAGCCAA AGACCGTCGT CTCCTCAGAG 
TCAACACTGG AGCCTAAAGA AGCAGCGACA GAAGAGACAG ATACTTTAAC AAGCCAAGAT 
GATGTAGAAA CACAACCATC AGATTTTGAT GCACTAAACA TTGATGATGT TGAACTTTTG 
CACATCCAAC ATGACTCTGA TTCTGAGTCA CAGCTTGAAA CACAAACAGC AGGTTTAGAT 
GTAGATATCG AAGAGCCTGC TAGCCATAAA GATTTACCAC TACAAACTTT AGAGAAAACA 
CTTGAGGATG AGGACAGCTG GTCATCAGCA TTAAATGAAA AAAGTGAATC GAAAGAGACT 
GAATCTTTTC ACGAACACGC CAAAGAATCA CTTTTAGTTC ATGACCTCAA AGCACAAAGA 
GATTTTGAAG AAGAGGAAAA TATTGATGAT GTGGAATTTT CTCATTCTCC TCACAATACG 
AAATCTGCTT CAGATGAGAG TGAAGTCGTC CCATCTGAAC CAGCTCTGGA AACATTAGCA 
TTAAATATAA GTGAAATCAC AGAGAATACA AAGGAAAATG CAGATGATTT TGTTATGAAG 
GCCAGCATGA ACACCAATGA AAATGCACAC AAAAACATTG CAGACGATGC TCGGGATGCA 
GCGATAAACA GAAGCCAACA GCCTACAGAG TCCATCGATG AAGATGAAAC TGAGAACGTT 
TCTGAAAGCC CAGAATCTGC TCCTCTACTT TATAAAACAG ACGAACAGAT CGAAAAGGTG 
AAGAACGAAC TGGAGGATCT GCTTAAAAAC ACACTGGAGA CTGAGGAAGA TAGTCCTGGA 
GGTGAAGAGG ACGAGGCTGA AGAGCTTCTG GAAGACGAAA ACGCTCTTCT TTCATCTAAT 
AGTCCTCACC TCACAGACCA AAATCCTGAA GACAATGAGC AACATGATGA TGATGGTTTG 
GTTGAATCTC AACAAAGCGA AAACACAACA CAAGCAGATG AAACTGAAAC TGAAGAGGTT 
GGTCTTCCTC CTGAAGAGCC TGAATACAGC GATAGCGTAC TGAGACTCAC AATATTGCGA 
GATCACTTGA AGGATGAGGA TACCGAGCGC ATGCAGAAGT ACTTCGGCCT GAAGAACATG 
TTCAGAATCG AAGCCATGTT CTCTGATCTG GATCTTGAGA TGAAGTCTGC TCGGGAGATG 
AAGACGGATA CGGAGGAGAT CGAGCAAACG CTGGACCAGA TCACGGAGGC TTCAGAAAAC 
TCCATTCTCG ATGCCACAGA GGACATACTT AACGACAGAG ACCGGAAAGC TCTGGAGGAT 
GGACTGCAGA AGCAACCGGA GATGTATGAT GTTGAGGCCG CCATGTTGGA CGCTTTTCAG 
GAGATCGTTT TCGCTTTGCG ACAGAAATAT TCGACAGCCA GCGACAGCGT CCCATTGGTC 
GAGGAGGAAC AAAGTGCATA TGAAAGAGAT GACCCCCGAG CTGACGCTGA AGAGGTGAAC 
AATCTGGACA TTACAACTGA AATAACTGAG CCAGCTGAAA CTCCTGAAAT GCACAAGGAG 
CAGAATGAAT CTGAACCGAT TCTGAATCCT GAATTGAACG TGAACCCAGA TTTGTCCCAC 
AATAAAGATG TGGATCTTGA GGAGGACGGA GGCCATTTCA ACAGGAATAA AGACGCTCAA 
ATCAGTTTCG AAGACGCTGA GGAGATCCAA AAGGGACCTC ATGCTATTTT GGAGAAGCCA 
GTGGACATTG GCTTTCACTT TGAGATGGAT CCGTCTTCAG GTTCGTTGGA GTCTCCGAGT 
GTCTCCGATT TCCATGATGC AGAAACTGGC AGTGATTCCT CCGACTCATC GACTTCAGAT 
GAACTTCAGG ATTTGGTGGT GCTTGTCAAA GAAAATCTGG GAGTTTATGG AGAGATTCTG 
ATCACTGCGC TTCCAGAGGA ATGGCGACCG GGTCCCAATT TTCACGGAGT CCCCTGGGAG 
CCAGTGGTGG TCACAGTGGC CATCGGGGCC CTCACTATAC TGATGTTCTT CTGGAGGACG 
GTGCTCGCTA TTAAAGGCAG AAGCTATCAG TTAACTGAAA AGCAGCTTAG AGACAAAATC 
CAGCAGCTTC TCAATGAGAA ATCTGATGCT GCGAACAAGA TCACAGAATT AAATGACATG 
ATAAAAGAAA GAGAACAACG CCTGCAAAAC TCAGAGAAGT CGCTGAGTTC CAGCCAACAT 
GAAATAAAAG GGCTGAAGAA TCATCACCAG AAAATGCAGA GTCAGTGGGA GCAGATGTCT 
GGGAGTATTT CGCAGCTCAA TCAGACAATT GTGGACACAC AGGAAGAAAA CGCAAACCTT 
AATGAAAAGA TTTCCAAGAT GCACCAGAGA ATTGAGAAAT ACCAGAAAAC ACTGAAGAAT 
TACGATGATG AGCGTGCAAA GGTTCATGTC CTTATGGATG AAGCTAAACT CCGAGAAGAC 
GCTCTTAAGG CTCAGGTTCT GTCTTTTGAG AAGGAAAATG GTGCTTTAAA AGAGCAGAAG 
AAATCTCTCC TTGGCGACGC TAAAGACTGG CAGGAGAAGC ACAAGAAGCT GAGCGAGGAG 
ATCCGAGTTT ATCACAAATC CCAGAAAGAG CTGGAAGACT CTCTGGTGCA CAAGGAGAAC 
GAGATCGATG TTTTGTCCAG CTGTATCGCC GAGCTCAACC GACTGGGAGG CTGCGACTCT 
GCAGAGCTAC AGAAAGAAGA TGCTAGAATG TCTAACGGGG ATGAATCTGA GAAGAAAATG 
GACACTATGA GACTGCGAAT CAAACAGATG ATGGACGTCT CCAGGATTAA AGCCACTCTC 
AGCGTTGTGG AGGACGAGAG GAATCGCTAT ATGGAAAATC TCCTCGCTGA ACAGAAATCC 
AGACAAGAGC TGGAAGAGCA ATATCAGAAG GTCATGCATG ACCAGATGAA TCTGAACAAT 
GAGAAAACGC ATCTGGAGAA CCAGATGAAG AACCTGCAGC AGAGACTGGA GATCACCACT 
GAACTCTACC AGCAGAAGGA GAACGCACTG CAACAAAAGT TGACTCAGGA GGAGCTGGAG 
AGACGCGAGA AGGAGACGAA GCTGTCGGAG GTGGACAGTA AAGCGCTGCG CTCGGAGGAG 
GAGGTTCGAG CTCTCAAGCA GAAGATAAAG GACATCGAGG AGGAGATGCA GCAGAACGAA 
CGCTCACTCA AATCTGAGGT GGCAGTGCAA GAGAAGAAGG CCCATGAGAA CTGGTTAAAA 
GCACGCGCCT CAGAGAGAAC TCTGGTGGAG GAGAGAAGAG AATCAGCCAC TCTTCGGCAG 
AAGCTTGTGG AATACCGAGA TAAAATATCC GACATGGAAC AAAGTCTGTT TAAGTTGAAC 
TCTGGGCCGC CAGATCGACA CATGCCGCCG CCACAGCGCA GAGGGGATTC GTACGGTCCA 
TCTCCTGTGA GCGGCGGGGC TCCGTCTCCT CCTTTGATGA TCGAGGGTCC AGGACGGCCC 
CCATCAGCAC CTGTAGGGCG ACGCGGTGAG CCGTTCGGTC CACGACCCCC GTCTGATCCT 
CACGGACGCT TCTCTGAGCT TGGACACCCG CTGCCATCCA GACCAGAAAT GTTTCAGCCG 
ATGACATCAT CTCCATGTGC TCATGATGGG CCGATGACGG CACCAGTCGT GGAGCCAGCC 
GAGTCCTCAG ATCAGGCCTC ATCTGAGCCT GTAGAGTCTC TGTCCAAATC TCAGAGTCAG 
GGATCCTTCC TGCCCTCCCC GATCCGAGAT TCTCCGGTTC CTCCACCTAA CGTCCCGCTG 
AAGCCTTACG GTCCCCCAGG GATGCCGCCC AATGGGCCAC CGATGATGAT CCGACCACCC 
AACGGACACC TTCCCATGAT GCCCCCCGAC CCGCGCTTCA GACCTCCACA CATGGACTCT 
TACGGCCCGC CTCCACACAT CGGCCCGTTT GGACCCGTGC CTCCACCTTT TGCTCGAGGG 
CCTCCACTGG GGCCGCCACT CGGACAGCGG GACGTCCCGC CAGATTTCTT TGCACCCCGC 
GGCCTCCCTC CTCGGCCCTT TCCGCCTGGA CCTCTGCCAC CCCCCGGAGC CATGATCCCT 
CCTCCATACA GCGGACACCG TTTTCCAGCA GCCCCTGCAG TACTGTCTCA GAGCTCCAGA 
GATGGAGACG AGGAGCCGCC GCAGACCCAC GGGGAGCCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mia3 ( XM_005160894.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160951.1 Danio rerio melanoma inhibitory activity family, member 3 (mia3), transcript variant X3, mRNA.

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CloneID	ODa25319
Clone ID Related Accession (Same CDS sequence)	XM_005160894.4 , XM_005160894.3
Accession Version	XM_005160894.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	melanoma inhibitory activity protein 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005160894.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAC CGAATAATCC CCGGTTTTAC TCACTTTTTA CCCTCCTCCT GTGCTCGTGC 
TTCGGCGGGA GCTGCTCGGA CCGGCGGTTC TCGGAGCTGA AGCGCTGCGC GGATGAAGAA 
TGCAGCATGC TTCTCGGCAG AGGAAAGGCC GCAAAGGATT TCACGGGGCC GGACTGTCGG 
TTTCTCTCTT TTAAGAAAGG AGAGACGATA TATGTATATT ATAAACTCTC AGGGCAGAGG 
TCAGACGTGT GGGCTGGTAG TGTTGGAAAC CACTTTGGCT ATTTTCCAAA AGACTATCTT 
AATATAAATC ATATATATAC TGAAAAAGAA TTGGAGGTAC CTACAGAAGA AACAGATTTT 
GTTTGCTTTG AGACTGGGCT TGATAAATTT GAAAGTTATG ACATAGATGT GTTATTAGGA 
AGCAAATTAT TTATGGAAAT TGAGAATTCA ACAGAAGAAT CAAAAGAAGC AGATCCGTAT 
GACATTTCTG AAACAGAAGA ATCAGTGGAG CATCTTGATT CAGAATCTCT GCTTGAATCA 
GAGGCAGTTC ATTCAGAATC AAGATTGCCT CTTCAATCAG AGTTGATCGA TTCAGAATCA 
GTATTGATTC TCCAACAAGA GTCAGTTGAT TCTGAATCAA AATTGCCTCT TGAATTAGAA 
TCAGTCAATT TAGAATCAGC ATCGACTCAT AAGTCAGTTA ATTCAGAAAC AGAATCGACT 
CATGAATCAG AGTTTGTTTA TTCGAAACCT GCATTGACTC CTGAATCAGA GTCAGTTGAT 
TCAGAATCAA GAACAAAGTC AATTGATTCA GAATCAACAA CACAGTTAAC TAATTCAGAA 
TCAACAACAC AGTCAACTGA TTCAGAATCA AGAACACAGT CAACTGATTC AGAATCAACA 
AAACAGTCAA CTAATTCAGA ATCAAGAACA CAGTCAACTG ATTCAGAATC AATGTTAAAG 
TCAACCGATT CAGAATTAAC CACACAGTCA ACCGATTTAG AATCAACTGA TTCAGAATCA 
ACAGCAGAGT CAACTGATTC AGAATCAACA ACACAGTCGA ATGATTTAGA ATCAACATCA 
CAGTCAACGG ATTCAGAATC AACAGCAGAG TCAAATGATT CAGAATCAGC AACACGGTCA 
AATGATTCAG GATCAACATT ACAGTCAAAT GATTCAGAAT CAGCAACACA GTCAAATGAT 
TCAGGATCAA CATTACAGTC AAATGCTTCA GAATCAACGT CGCAGTCAGC AGATTCAGAA 
TCAACAACAC AGCCAATTGA TTCAGAATCA ACATTGGCTC TTGAATCAGA ATCATTTGAG 
TCAGAATTAA AATCATTACC TCTTGAGTCA GAGTCATTTG ATTCAGAATC AACACTGCCT 
CTTAAATCAG AGTCAGTAGA TTCAGAATCT GTAGAAGCTT CTGAATCAGA CACATTGATT 
CCTAAATCTA CTGAAGATGA CTCTGATAGC TCTCAGCCAA AGACCGTCGT CTCCTCAGAG 
TCAACACTGG AGCCTAAAGA AGCAGCGACA GAAGAGACAG ATACTTTAAC AAGCCAAGAT 
GATGTAGAAA CACAACCATC AGATTTTGAT GCACTAAACA TTGATGATGT TGAACTTTTG 
CACATCCAAC ATGACTCTGA TTCTGAGTCA CAGCTTGAAA CACAAACAGC AGGTTTAGAT 
GTAGATATCG AAGAGCCTGC TAGCCATAAA GATTTACCAC TACAAACTTT AGAGAAAACA 
CTTGAGGATG AGGACAGCTG GTCATCAGCA TTAAATGAAA AAAGTGAATC GAAAGAGACT 
GAATCTTTTC ACGAACACGC CAAAGAATCA CTTTTAGTTC ATGACCTCAA AGCACAAAGA 
GATTTTGAAG AAGAGGAAAA TATTGATGAT GTGGAATTTT CTCATTCTCC TCACAATACG 
AAATCTGCTT CAGATGAGAG TGAAGTCGTC CCATCTGAAC CAGCTCTGGA AACATTAGCA 
TTAAATATAA GTGAAATCAC AGAGAATACA AAGGAAAATG CAGATGATTT TGTTATGAAG 
GCCAGCATGA ACACCAATGA AAATGCACAC AAAAACATTG CAGACGATGC TCGGGATGCA 
GCGATAAACA GAAGCCAACA GCCTACAGAG TCCATCGATG AAGATGAAAC TGAGAACGTT 
TCTGAAAGCC CAGAATCTGC TCCTCTACTT TATAAAACAG ACGAACAGAT CGAAAAGGTG 
AAGAACGAAC TGGAGGATCT GCTTAAAAAC ACACTGGAGA CTGAGGAAGA TAGTCCTGGA 
GGTGAAGAGG ACGAGGCTGA AGAGCTTCTG GAAGACGAAA ACGCTCTTCT TTCATCTAAT 
AGTCCTCACC TCACAGACCA AAATCCTGAA GACAATGAGC AACATGATGA TGATGGTTTG 
GTTGAATCTC AACAAAGCGA AAACACAACA CAAGCAGATG AAACTGAAAC TGAAGAGGTT 
GGTCTTCCTC CTGAAGAGCC TGAATACAGC GATAGCGTAC TGAGACTCAC AATATTGCGA 
GATCACTTGA AGGATGAGGA TACCGAGCGC ATGCAGAAGT ACTTCGGCCT GAAGAACATG 
TTCAGAATCG AAGCCATGTT CTCTGATCTG GATCTTGAGA TGAAGTCTGC TCGGGAGATG 
AAGACGGATA CGGAGGAGAT CGAGCAAACG CTGGACCAGA TCACGGAGGC TTCAGAAAAC 
TCCATTCTCG ATGCCACAGA GGACATACTT AACGACAGAG ACCGGAAAGC TCTGGAGGAT 
GGACTGCAGA AGCAACCGGA GATGTATGAT GTTGAGGCCG CCATGTTGGA CGCTTTTCAG 
GAGATCGTTT TCGCTTTGCG ACAGAAATAT TCGACAGCCA GCGACAGCGT CCCATTGGTC 
GAGGAGGAAC AAAGTGCATA TGAAAGAGAT GACCCCCGAG CTGACGCTGA AGAGGTGAAC 
AATCTGGACA TTACAACTGA AATAACTGAG CCAGCTGAAA CTCCTGAAAT GCACAAGGAG 
CAGAATGAAT CTGAACCGAT TCTGAATCCT GAATTGAACG TGAACCCAGA TTTGTCCCAC 
AATAAAGATG TGGATCTTGA GGAGGACGGA GGCCATTTCA ACAGGAATAA AGACGCTCAA 
ATCAGTTTCG AAGACGCTGA GGAGATCCAA AAGGGACCTC ATGCTATTTT GGAGAAGCCA 
GTGGACATTG GCTTTCACTT TGAGATGGAT CCGTCTTCAG GTTCGTTGGA GTCTCCGAGT 
GTCTCCGATT TCCATGATGC AGAAACTGGC AGTGATTCCT CCGACTCATC GACTTCAGAT 
GAACTTCAGG ATTTGGTGGT GCTTGTCAAA GAAAATCTGG GAGTTTATGG AGAGATTCTG 
ATCACTGCGC TTCCAGAGGA ATGGCGACCG GGTCCCAATT TTCACGGAGT CCCCTGGGAG 
CCAGTGGTGG TCACAGTGGC CATCGGGGCC CTCACTATAC TGATGTTCTT CTGGAGGACG 
GTGCTCGCTA TTAAAGGCAG AAGCTATCAG TTAACTGAAA AGCAGCTTAG AGACAAAATC 
CAGCAGCTTC TCAATGAGAA ATCTGATGCT GCGAACAAGA TCACAGAATT AAATGACATG 
ATAAAAGAAA GAGAACAACG CCTGCAAAAC TCAGAGAAGT CGCTGAGTTC CAGCCAACAT 
GAAATAAAAG GGCTGAAGAA TCATCACCAG AAAATGCAGA GTCAGTGGGA GCAGATGTCT 
GGGAGTATTT CGCAGCTCAA TCAGACAATT GTGGACACAC AGGAAGAAAA CGCAAACCTT 
AATGAAAAGA TTTCCAAGAT GCACCAGAGA ATTGAGAAAT ACCAGAAAAC ACTGAAGAAT 
TACGATGATG AGCGTGCAAA GGTTCATGTC CTTATGGATG AAGCTAAACT CCGAGAAGAC 
GCTCTTAAGG CTCAGGTTCT GTCTTTTGAG AAGGAAAATG GTGCTTTAAA AGAGCAGAAG 
AAATCTCTCC TTGGCGACGC TAAAGACTGG CAGGAGAAGC ACAAGAAGCT GAGCGAGGAG 
ATCCGAGTTT ATCACAAATC CCAGAAAGAG CTGGAAGACT CTCTGGTGCA CAAGGAGAAC 
GAGATCGATG TTTTGTCCAG CTGTATCGCC GAGCTCAACC GACTGGGAGG CTGCGACTCT 
GCAGAGCTAC AGAAAGAAGA TGCTAGAATG TCTAACGGGG ATGAATCTGA GAAGAAAATG 
GACACTATGA GACTGCGAAT CAAACAGATG ATGGACGTCT CCAGGATTAA AGCCACTCTC 
AGCGTTGTGG AGGACGAGAG GAATCGCTAT ATGGAAAATC TCCTCGCTGA ACAGAAATCC 
AGACAAGAGC TGGAAGAGCA ATATCAGAAG GTCATGCATG ACCAGATGAA TCTGAACAAT 
GAGAAAACGC ATCTGGAGAA CCAGATGAAG AACCTGCAGC AGAGACTGGA GATCACCACT 
GAACTCTACC AGCAGAAGGA GAACGCACTG CAACAAAAGT TGACTCAGGA GGAGCTGGAG 
AGACGCGAGA AGGAGACGAA GCTGTCGGAG GTGGACAGTA AAGCGCTGCG CTCGGAGGAG 
GAGGTTCGAG CTCTCAAGCA GAAGATAAAG GACATCGAGG AGGAGATGCA GCAGAACGAA 
CGCTCACTCA AATCTGAGGT GGCAGTGCAA GAGAAGAAGG CCCATGAGAA CTGGTTAAAA 
GCACGCGCCT CAGAGAGAAC TCTGGTGGAG GAGAGAAGAG AATCAGCCAC TCTTCGGCAG 
AAGCTTGTGG AATACCGAGA TAAAATATCC GACATGGAAC AAAGTCTGTT TAAGTTGAAC 
TCTGGGCCGC CAGATCGACA CATGCCGCCG CCACAGCGCA GAGGGGATTC GTACGGTCCA 
TCTCCTGTGA GCGGCGGGGC TCCGTCTCCT CCTTTGATGA TCGAGGGTCC AGGACGGCCC 
CCATCAGCAC CTGTAGGGCG ACGCGGTGAG CCGTTCGGTC CACGACCCCC GTCTGATCCT 
CACGGACGCT TCTCTGAGCT TGGACACCCG CTGCCATCCA GACCAGAAAT GTTTCAGCCG 
ATGACATCAT CTCCATGTGC TCATGATGGG CCGATGCTGT CCAAATCTCA GAGTCAGGGA 
TCCTTCCTGC CCTCCCCGAT CCGAGATTCT CCGGTTCCTC CACCTAACGT CCCGCTGAAG 
CCTTACGGTC CCCCAGGGAT GCCGCCCAAT GGGCCACCGA TGATGATCCG ACCACCCAAC 
GGACACCTTC CCATGATGCC CCCCGACCCG CGCTTCAGAC CTCCACACAT GGACTCTTAC 
GGCCCGCCTC CACACATCGG CCCGTTTGGA CCCGTGCCTC CACCTTTTGC TCGAGGGCCT 
CCACTGGGGC CGCCACTCGG ACAGCGGGAC GTCCCGCCAG ATTTCTTTGC ACCCCGCGGC 
CTCCCTCCTC GGCCCTTTCC GCCTGGACCT CTGCCACCCC CCGGAGCCAT GATCCCTCCT 
CCATACAGCG GACACCGTTT TCCAGCAGCC CCTGCAGTAC TGTCTCAGAG CTCCAGAGAT 
GGAGACGAGG AGCCGCCGCA GACCCACGGG GAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160951.1
CDS	152..5710
Translation	MAAPNNPRFYSLFTLLLCSCFGGSCSDRRFSELKRCADEECSMLLGRGKAAKDFTGPDCRFLSFKKGETIYVYYKLSGQRSDVWAGSVGNHFGYFPKDYLNINHIYTEKELEVPTEETDFVCFETGLDKFESYDIDVLLGSKLFMEIENSTEESKEADPYDISETEESVEHLDSESLLESEAVHSESRLPLQSELIDSESVLILQQESVDSESKLPLELESVNLESASTHKSVNSETESTHESEFVYSKPALTPESESVDSESRTKSIDSESTTQLTNSESTTQSTDSESRTQSTDSESTKQSTNSESRTQSTDSESMLKSTDSELTTQSTDLESTDSESTAESTDSESTTQSNDLESTSQSTDSESTAESNDSESATRSNDSGSTLQSNDSESATQSNDSGSTLQSNASESTSQSADSESTTQPIDSESTLALESESFESELKSLPLESESFDSESTLPLKSESVDSESVEASESDTLIPKSTEDDSDSSQPKTVVSSESTLEPKEAATEETDTLTSQDDVETQPSDFDALNIDDVELLHIQHDSDSESQLETQTAGLDVDIEEPASHKDLPLQTLEKTLEDEDSWSSALNEKSESKETESFHEHAKESLLVHDLKAQRDFEEEENIDDVEFSHSPHNTKSASDESEVVPSEPALETLALNISEITENTKENADDFVMKASMNTNENAHKNIADDARDAAINRSQQPTESIDEDETENVSESPESAPLLYKTDEQIEKVKNELEDLLKNTLETEEDSPGGEEDEAEELLEDENALLSSNSPHLTDQNPEDNEQHDDDGLVESQQSENTTQADETETEEVGLPPEEPEYSDSVLRLTILRDHLKDEDTERMQKYFGLKNMFRIEAMFSDLDLEMKSAREMKTDTEEIEQTLDQITEASENSILDATEDILNDRDRKALEDGLQKQPEMYDVEAAMLDAFQEIVFALRQKYSTASDSVPLVEEEQSAYERDDPRADAEEVNNLDITTEITEPAETPEMHKEQNESEPILNPELNVNPDLSHNKDVDLEEDGGHFNRNKDAQISFEDAEEIQKGPHAILEKPVDIGFHFEMDPSSGSLESPSVSDFHDAETGSDSSDSSTSDELQDLVVLVKENLGVYGEILITALPEEWRPGPNFHGVPWEPVVVTVAIGALTILMFFWRTVLAIKGRSYQLTEKQLRDKIQQLLNEKSDAANKITELNDMIKEREQRLQNSEKSLSSSQHEIKGLKNHHQKMQSQWEQMSGSISQLNQTIVDTQEENANLNEKISKMHQRIEKYQKTLKNYDDERAKVHVLMDEAKLREDALKAQVLSFEKENGALKEQKKSLLGDAKDWQEKHKKLSEEIRVYHKSQKELEDSLVHKENEIDVLSSCIAELNRLGGCDSAELQKEDARMSNGDESEKKMDTMRLRIKQMMDVSRIKATLSVVEDERNRYMENLLAEQKSRQELEEQYQKVMHDQMNLNNEKTHLENQMKNLQQRLEITTELYQQKENALQQKLTQEELERREKETKLSEVDSKALRSEEEVRALKQKIKDIEEEMQQNERSLKSEVAVQEKKAHENWLKARASERTLVEERRESATLRQKLVEYRDKISDMEQSLFKLNSGPPDRHMPPPQRRGDSYGPSPVSGGAPSPPLMIEGPGRPPSAPVGRRGEPFGPRPPSDPHGRFSELGHPLPSRPEMFQPMTSSPCAHDGPMLSKSQSQGSFLPSPIRDSPVPPPNVPLKPYGPPGMPPNGPPMMIRPPNGHLPMMPPDPRFRPPHMDSYGPPPHIGPFGPVPPPFARGPPLGPPLGQRDVPPDFFAPRGLPPRPFPPGPLPPPGAMIPPPYSGHRFPAAPAVLSQSSRDGDEEPPQTHGEP

Target ORF information:

RefSeq Version	XM_005160894.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio melanoma inhibitory activity family, member 3 (mia3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160894.4

ORF Insert Sequence:

ATGGCTGCAC CGAATAATCC CCGGTTTTAC TCACTTTTTA CCCTCCTCCT GTGCTCGTGC 
TTCGGCGGGA GCTGCTCGGA CCGGCGGTTC TCGGAGCTGA AGCGCTGCGC GGATGAAGAA 
TGCAGCATGC TTCTCGGCAG AGGAAAGGCC GCAAAGGATT TCACGGGGCC GGACTGTCGG 
TTTCTCTCTT TTAAGAAAGG AGAGACGATA TATGTATATT ATAAACTCTC AGGGCAGAGG 
TCAGACGTGT GGGCTGGTAG TGTTGGAAAC CACTTTGGCT ATTTTCCAAA AGACTATCTT 
AATATAAATC ATATATATAC TGAAAAAGAA TTGGAGGTAC CTACAGAAGA AACAGATTTT 
GTTTGCTTTG AGACTGGGCT TGATAAATTT GAAAGTTATG ACATAGATGT GTTATTAGGA 
AGCAAATTAT TTATGGAAAT TGAGAATTCA ACAGAAGAAT CAAAAGAAGC AGATCCGTAT 
GACATTTCTG AAACAGAAGA ATCAGTGGAG CATCTTGATT CAGAATCTCT GCTTGAATCA 
GAGGCAGTTC ATTCAGAATC AAGATTGCCT CTTCAATCAG AGTTGATCGA TTCAGAATCA 
GTATTGATTC TCCAACAAGA GTCAGTTGAT TCTGAATCAA AATTGCCTCT TGAATTAGAA 
TCAGTCAATT TAGAATCAGC ATCGACTCAT AAGTCAGTTA ATTCAGAAAC AGAATCGACT 
CATGAATCAG AGTTTGTTTA TTCGAAACCT GCATTGACTC CTGAATCAGA GTCAGTTGAT 
TCAGAATCAA GAACAAAGTC AATTGATTCA GAATCAACAA CACAGTTAAC TAATTCAGAA 
TCAACAACAC AGTCAACTGA TTCAGAATCA AGAACACAGT CAACTGATTC AGAATCAACA 
AAACAGTCAA CTAATTCAGA ATCAAGAACA CAGTCAACTG ATTCAGAATC AATGTTAAAG 
TCAACCGATT CAGAATTAAC CACACAGTCA ACCGATTTAG AATCAACTGA TTCAGAATCA 
ACAGCAGAGT CAACTGATTC AGAATCAACA ACACAGTCGA ATGATTTAGA ATCAACATCA 
CAGTCAACGG ATTCAGAATC AACAGCAGAG TCAAATGATT CAGAATCAGC AACACGGTCA 
AATGATTCAG GATCAACATT ACAGTCAAAT GATTCAGAAT CAGCAACACA GTCAAATGAT 
TCAGGATCAA CATTACAGTC AAATGCTTCA GAATCAACGT CGCAGTCAGC AGATTCAGAA 
TCAACAACAC AGCCAATTGA TTCAGAATCA ACATTGGCTC TTGAATCAGA ATCATTTGAG 
TCAGAATTAA AATCATTACC TCTTGAGTCA GAGTCATTTG ATTCAGAATC AACACTGCCT 
CTTAAATCAG AGTCAGTAGA TTCAGAATCT GTAGAAGCTT CTGAATCAGA CACATTGATT 
CCTAAATCTA CTGAAGATGA CTCTGATAGC TCTCAGCCAA AGACCGTCGT CTCCTCAGAG 
TCAACACTGG AGCCTAAAGA AGCAGCGACA GAAGAGACAG ATACTTTAAC AAGCCAAGAT 
GATGTAGAAA CACAACCATC AGATTTTGAT GCACTAAACA TTGATGATGT TGAACTTTTG 
CACATCCAAC ATGACTCTGA TTCTGAGTCA CAGCTTGAAA CACAAACAGC AGGTTTAGAT 
GTAGATATCG AAGAGCCTGC TAGCCATAAA GATTTACCAC TACAAACTTT AGAGAAAACA 
CTTGAGGATG AGGACAGCTG GTCATCAGCA TTAAATGAAA AAAGTGAATC GAAAGAGACT 
GAATCTTTTC ACGAACACGC CAAAGAATCA CTTTTAGTTC ATGACCTCAA AGCACAAAGA 
GATTTTGAAG AAGAGGAAAA TATTGATGAT GTGGAATTTT CTCATTCTCC TCACAATACG 
AAATCTGCTT CAGATGAGAG TGAAGTCGTC CCATCTGAAC CAGCTCTGGA AACATTAGCA 
TTAAATATAA GTGAAATCAC AGAGAATACA AAGGAAAATG CAGATGATTT TGTTATGAAG 
GCCAGCATGA ACACCAATGA AAATGCACAC AAAAACATTG CAGACGATGC TCGGGATGCA 
GCGATAAACA GAAGCCAACA GCCTACAGAG TCCATCGATG AAGATGAAAC TGAGAACGTT 
TCTGAAAGCC CAGAATCTGC TCCTCTACTT TATAAAACAG ACGAACAGAT CGAAAAGGTG 
AAGAACGAAC TGGAGGATCT GCTTAAAAAC ACACTGGAGA CTGAGGAAGA TAGTCCTGGA 
GGTGAAGAGG ACGAGGCTGA AGAGCTTCTG GAAGACGAAA ACGCTCTTCT TTCATCTAAT 
AGTCCTCACC TCACAGACCA AAATCCTGAA GACAATGAGC AACATGATGA TGATGGTTTG 
GTTGAATCTC AACAAAGCGA AAACACAACA CAAGCAGATG AAACTGAAAC TGAAGAGGTT 
GGTCTTCCTC CTGAAGAGCC TGAATACAGC GATAGCGTAC TGAGACTCAC AATATTGCGA 
GATCACTTGA AGGATGAGGA TACCGAGCGC ATGCAGAAGT ACTTCGGCCT GAAGAACATG 
TTCAGAATCG AAGCCATGTT CTCTGATCTG GATCTTGAGA TGAAGTCTGC TCGGGAGATG 
AAGACGGATA CGGAGGAGAT CGAGCAAACG CTGGACCAGA TCACGGAGGC TTCAGAAAAC 
TCCATTCTCG ATGCCACAGA GGACATACTT AACGACAGAG ACCGGAAAGC TCTGGAGGAT 
GGACTGCAGA AGCAACCGGA GATGTATGAT GTTGAGGCCG CCATGTTGGA CGCTTTTCAG 
GAGATCGTTT TCGCTTTGCG ACAGAAATAT TCGACAGCCA GCGACAGCGT CCCATTGGTC 
GAGGAGGAAC AAAGTGCATA TGAAAGAGAT GACCCCCGAG CTGACGCTGA AGAGGTGAAC 
AATCTGGACA TTACAACTGA AATAACTGAG CCAGCTGAAA CTCCTGAAAT GCACAAGGAG 
CAGAATGAAT CTGAACCGAT TCTGAATCCT GAATTGAACG TGAACCCAGA TTTGTCCCAC 
AATAAAGATG TGGATCTTGA GGAGGACGGA GGCCATTTCA ACAGGAATAA AGACGCTCAA 
ATCAGTTTCG AAGACGCTGA GGAGATCCAA AAGGGACCTC ATGCTATTTT GGAGAAGCCA 
GTGGACATTG GCTTTCACTT TGAGATGGAT CCGTCTTCAG GTTCGTTGGA GTCTCCGAGT 
GTCTCCGATT TCCATGATGC AGAAACTGGC AGTGATTCCT CCGACTCATC GACTTCAGAT 
GAACTTCAGG ATTTGGTGGT GCTTGTCAAA GAAAATCTGG GAGTTTATGG AGAGATTCTG 
ATCACTGCGC TTCCAGAGGA ATGGCGACCG GGTCCCAATT TTCACGGAGT CCCCTGGGAG 
CCAGTGGTGG TCACAGTGGC CATCGGGGCC CTCACTATAC TGATGTTCTT CTGGAGGACG 
GTGCTCGCTA TTAAAGGCAG AAGCTATCAG TTAACTGAAA AGCAGCTTAG AGACAAAATC 
CAGCAGCTTC TCAATGAGAA ATCTGATGCT GCGAACAAGA TCACAGAATT AAATGACATG 
ATAAAAGAAA GAGAACAACG CCTGCAAAAC TCAGAGAAGT CGCTGAGTTC CAGCCAACAT 
GAAATAAAAG GGCTGAAGAA TCATCACCAG AAAATGCAGA GTCAGTGGGA GCAGATGTCT 
GGGAGTATTT CGCAGCTCAA TCAGACAATT GTGGACACAC AGGAAGAAAA CGCAAACCTT 
AATGAAAAGA TTTCCAAGAT GCACCAGAGA ATTGAGAAAT ACCAGAAAAC ACTGAAGAAT 
TACGATGATG AGCGTGCAAA GGTTCATGTC CTTATGGATG AAGCTAAACT CCGAGAAGAC 
GCTCTTAAGG CTCAGGTTCT GTCTTTTGAG AAGGAAAATG GTGCTTTAAA AGAGCAGAAG 
AAATCTCTCC TTGGCGACGC TAAAGACTGG CAGGAGAAGC ACAAGAAGCT GAGCGAGGAG 
ATCCGAGTTT ATCACAAATC CCAGAAAGAG CTGGAAGACT CTCTGGTGCA CAAGGAGAAC 
GAGATCGATG TTTTGTCCAG CTGTATCGCC GAGCTCAACC GACTGGGAGG CTGCGACTCT 
GCAGAGCTAC AGAAAGAAGA TGCTAGAATG TCTAACGGGG ATGAATCTGA GAAGAAAATG 
GACACTATGA GACTGCGAAT CAAACAGATG ATGGACGTCT CCAGGATTAA AGCCACTCTC 
AGCGTTGTGG AGGACGAGAG GAATCGCTAT ATGGAAAATC TCCTCGCTGA ACAGAAATCC 
AGACAAGAGC TGGAAGAGCA ATATCAGAAG GTCATGCATG ACCAGATGAA TCTGAACAAT 
GAGAAAACGC ATCTGGAGAA CCAGATGAAG AACCTGCAGC AGAGACTGGA GATCACCACT 
GAACTCTACC AGCAGAAGGA GAACGCACTG CAACAAAAGT TGACTCAGGA GGAGCTGGAG 
AGACGCGAGA AGGAGACGAA GCTGTCGGAG GTGGACAGTA AAGCGCTGCG CTCGGAGGAG 
GAGGTTCGAG CTCTCAAGCA GAAGATAAAG GACATCGAGG AGGAGATGCA GCAGAACGAA 
CGCTCACTCA AATCTGAGGT GGCAGTGCAA GAGAAGAAGG CCCATGAGAA CTGGTTAAAA 
GCACGCGCCT CAGAGAGAAC TCTGGTGGAG GAGAGAAGAG AATCAGCCAC TCTTCGGCAG 
AAGCTTGTGG AATACCGAGA TAAAATATCC GACATGGAAC AAAGTCTGTT TAAGTTGAAC 
TCTGGGCCGC CAGATCGACA CATGCCGCCG CCACAGCGCA GAGGGGATTC GTACGGTCCA 
TCTCCTGTGA GCGGCGGGGC TCCGTCTCCT CCTTTGATGA TCGAGGGTCC AGGACGGCCC 
CCATCAGCAC CTGTAGGGCG ACGCGGTGAG CCGTTCGGTC CACGACCCCC GTCTGATCCT 
CACGGACGCT TCTCTGAGCT TGGACACCCG CTGCCATCCA GACCAGAAAT GTTTCAGCCG 
ATGACATCAT CTCCATGTGC TCATGATGGG CCGATGCTGT CCAAATCTCA GAGTCAGGGA 
TCCTTCCTGC CCTCCCCGAT CCGAGATTCT CCGGTTCCTC CACCTAACGT CCCGCTGAAG 
CCTTACGGTC CCCCAGGGAT GCCGCCCAAT GGGCCACCGA TGATGATCCG ACCACCCAAC 
GGACACCTTC CCATGATGCC CCCCGACCCG CGCTTCAGAC CTCCACACAT GGACTCTTAC 
GGCCCGCCTC CACACATCGG CCCGTTTGGA CCCGTGCCTC CACCTTTTGC TCGAGGGCCT 
CCACTGGGGC CGCCACTCGG ACAGCGGGAC GTCCCGCCAG ATTTCTTTGC ACCCCGCGGC 
CTCCCTCCTC GGCCCTTTCC GCCTGGACCT CTGCCACCCC CCGGAGCCAT GATCCCTCCT 
CCATACAGCG GACACCGTTT TCCAGCAGCC CCTGCAGTAC TGTCTCAGAG CTCCAGAGAT 
GGAGACGAGG AGCCGCCGCA GACCCACGGG GAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mia3 ( XM_005160894.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160951.1 Danio rerio melanoma inhibitory activity family, member 3 (mia3), transcript variant X3, mRNA.

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CloneID	ODa25319
Clone ID Related Accession (Same CDS sequence)	XM_005160894.4 , XM_005160894.3
Accession Version	XM_005160894.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	melanoma inhibitory activity protein 3 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005160894.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAC CGAATAATCC CCGGTTTTAC TCACTTTTTA CCCTCCTCCT GTGCTCGTGC 
TTCGGCGGGA GCTGCTCGGA CCGGCGGTTC TCGGAGCTGA AGCGCTGCGC GGATGAAGAA 
TGCAGCATGC TTCTCGGCAG AGGAAAGGCC GCAAAGGATT TCACGGGGCC GGACTGTCGG 
TTTCTCTCTT TTAAGAAAGG AGAGACGATA TATGTATATT ATAAACTCTC AGGGCAGAGG 
TCAGACGTGT GGGCTGGTAG TGTTGGAAAC CACTTTGGCT ATTTTCCAAA AGACTATCTT 
AATATAAATC ATATATATAC TGAAAAAGAA TTGGAGGTAC CTACAGAAGA AACAGATTTT 
GTTTGCTTTG AGACTGGGCT TGATAAATTT GAAAGTTATG ACATAGATGT GTTATTAGGA 
AGCAAATTAT TTATGGAAAT TGAGAATTCA ACAGAAGAAT CAAAAGAAGC AGATCCGTAT 
GACATTTCTG AAACAGAAGA ATCAGTGGAG CATCTTGATT CAGAATCTCT GCTTGAATCA 
GAGGCAGTTC ATTCAGAATC AAGATTGCCT CTTCAATCAG AGTTGATCGA TTCAGAATCA 
GTATTGATTC TCCAACAAGA GTCAGTTGAT TCTGAATCAA AATTGCCTCT TGAATTAGAA 
TCAGTCAATT TAGAATCAGC ATCGACTCAT AAGTCAGTTA ATTCAGAAAC AGAATCGACT 
CATGAATCAG AGTTTGTTTA TTCGAAACCT GCATTGACTC CTGAATCAGA GTCAGTTGAT 
TCAGAATCAA GAACAAAGTC AATTGATTCA GAATCAACAA CACAGTTAAC TAATTCAGAA 
TCAACAACAC AGTCAACTGA TTCAGAATCA AGAACACAGT CAACTGATTC AGAATCAACA 
AAACAGTCAA CTAATTCAGA ATCAAGAACA CAGTCAACTG ATTCAGAATC AATGTTAAAG 
TCAACCGATT CAGAATTAAC CACACAGTCA ACCGATTTAG AATCAACTGA TTCAGAATCA 
ACAGCAGAGT CAACTGATTC AGAATCAACA ACACAGTCGA ATGATTTAGA ATCAACATCA 
CAGTCAACGG ATTCAGAATC AACAGCAGAG TCAAATGATT CAGAATCAGC AACACGGTCA 
AATGATTCAG GATCAACATT ACAGTCAAAT GATTCAGAAT CAGCAACACA GTCAAATGAT 
TCAGGATCAA CATTACAGTC AAATGCTTCA GAATCAACGT CGCAGTCAGC AGATTCAGAA 
TCAACAACAC AGCCAATTGA TTCAGAATCA ACATTGGCTC TTGAATCAGA ATCATTTGAG 
TCAGAATTAA AATCATTACC TCTTGAGTCA GAGTCATTTG ATTCAGAATC AACACTGCCT 
CTTAAATCAG AGTCAGTAGA TTCAGAATCT GTAGAAGCTT CTGAATCAGA CACATTGATT 
CCTAAATCTA CTGAAGATGA CTCTGATAGC TCTCAGCCAA AGACCGTCGT CTCCTCAGAG 
TCAACACTGG AGCCTAAAGA AGCAGCGACA GAAGAGACAG ATACTTTAAC AAGCCAAGAT 
GATGTAGAAA CACAACCATC AGATTTTGAT GCACTAAACA TTGATGATGT TGAACTTTTG 
CACATCCAAC ATGACTCTGA TTCTGAGTCA CAGCTTGAAA CACAAACAGC AGGTTTAGAT 
GTAGATATCG AAGAGCCTGC TAGCCATAAA GATTTACCAC TACAAACTTT AGAGAAAACA 
CTTGAGGATG AGGACAGCTG GTCATCAGCA TTAAATGAAA AAAGTGAATC GAAAGAGACT 
GAATCTTTTC ACGAACACGC CAAAGAATCA CTTTTAGTTC ATGACCTCAA AGCACAAAGA 
GATTTTGAAG AAGAGGAAAA TATTGATGAT GTGGAATTTT CTCATTCTCC TCACAATACG 
AAATCTGCTT CAGATGAGAG TGAAGTCGTC CCATCTGAAC CAGCTCTGGA AACATTAGCA 
TTAAATATAA GTGAAATCAC AGAGAATACA AAGGAAAATG CAGATGATTT TGTTATGAAG 
GCCAGCATGA ACACCAATGA AAATGCACAC AAAAACATTG CAGACGATGC TCGGGATGCA 
GCGATAAACA GAAGCCAACA GCCTACAGAG TCCATCGATG AAGATGAAAC TGAGAACGTT 
TCTGAAAGCC CAGAATCTGC TCCTCTACTT TATAAAACAG ACGAACAGAT CGAAAAGGTG 
AAGAACGAAC TGGAGGATCT GCTTAAAAAC ACACTGGAGA CTGAGGAAGA TAGTCCTGGA 
GGTGAAGAGG ACGAGGCTGA AGAGCTTCTG GAAGACGAAA ACGCTCTTCT TTCATCTAAT 
AGTCCTCACC TCACAGACCA AAATCCTGAA GACAATGAGC AACATGATGA TGATGGTTTG 
GTTGAATCTC AACAAAGCGA AAACACAACA CAAGCAGATG AAACTGAAAC TGAAGAGGTT 
GGTCTTCCTC CTGAAGAGCC TGAATACAGC GATAGCGTAC TGAGACTCAC AATATTGCGA 
GATCACTTGA AGGATGAGGA TACCGAGCGC ATGCAGAAGT ACTTCGGCCT GAAGAACATG 
TTCAGAATCG AAGCCATGTT CTCTGATCTG GATCTTGAGA TGAAGTCTGC TCGGGAGATG 
AAGACGGATA CGGAGGAGAT CGAGCAAACG CTGGACCAGA TCACGGAGGC TTCAGAAAAC 
TCCATTCTCG ATGCCACAGA GGACATACTT AACGACAGAG ACCGGAAAGC TCTGGAGGAT 
GGACTGCAGA AGCAACCGGA GATGTATGAT GTTGAGGCCG CCATGTTGGA CGCTTTTCAG 
GAGATCGTTT TCGCTTTGCG ACAGAAATAT TCGACAGCCA GCGACAGCGT CCCATTGGTC 
GAGGAGGAAC AAAGTGCATA TGAAAGAGAT GACCCCCGAG CTGACGCTGA AGAGGTGAAC 
AATCTGGACA TTACAACTGA AATAACTGAG CCAGCTGAAA CTCCTGAAAT GCACAAGGAG 
CAGAATGAAT CTGAACCGAT TCTGAATCCT GAATTGAACG TGAACCCAGA TTTGTCCCAC 
AATAAAGATG TGGATCTTGA GGAGGACGGA GGCCATTTCA ACAGGAATAA AGACGCTCAA 
ATCAGTTTCG AAGACGCTGA GGAGATCCAA AAGGGACCTC ATGCTATTTT GGAGAAGCCA 
GTGGACATTG GCTTTCACTT TGAGATGGAT CCGTCTTCAG GTTCGTTGGA GTCTCCGAGT 
GTCTCCGATT TCCATGATGC AGAAACTGGC AGTGATTCCT CCGACTCATC GACTTCAGAT 
GAACTTCAGG ATTTGGTGGT GCTTGTCAAA GAAAATCTGG GAGTTTATGG AGAGATTCTG 
ATCACTGCGC TTCCAGAGGA ATGGCGACCG GGTCCCAATT TTCACGGAGT CCCCTGGGAG 
CCAGTGGTGG TCACAGTGGC CATCGGGGCC CTCACTATAC TGATGTTCTT CTGGAGGACG 
GTGCTCGCTA TTAAAGGCAG AAGCTATCAG TTAACTGAAA AGCAGCTTAG AGACAAAATC 
CAGCAGCTTC TCAATGAGAA ATCTGATGCT GCGAACAAGA TCACAGAATT AAATGACATG 
ATAAAAGAAA GAGAACAACG CCTGCAAAAC TCAGAGAAGT CGCTGAGTTC CAGCCAACAT 
GAAATAAAAG GGCTGAAGAA TCATCACCAG AAAATGCAGA GTCAGTGGGA GCAGATGTCT 
GGGAGTATTT CGCAGCTCAA TCAGACAATT GTGGACACAC AGGAAGAAAA CGCAAACCTT 
AATGAAAAGA TTTCCAAGAT GCACCAGAGA ATTGAGAAAT ACCAGAAAAC ACTGAAGAAT 
TACGATGATG AGCGTGCAAA GGTTCATGTC CTTATGGATG AAGCTAAACT CCGAGAAGAC 
GCTCTTAAGG CTCAGGTTCT GTCTTTTGAG AAGGAAAATG GTGCTTTAAA AGAGCAGAAG 
AAATCTCTCC TTGGCGACGC TAAAGACTGG CAGGAGAAGC ACAAGAAGCT GAGCGAGGAG 
ATCCGAGTTT ATCACAAATC CCAGAAAGAG CTGGAAGACT CTCTGGTGCA CAAGGAGAAC 
GAGATCGATG TTTTGTCCAG CTGTATCGCC GAGCTCAACC GACTGGGAGG CTGCGACTCT 
GCAGAGCTAC AGAAAGAAGA TGCTAGAATG TCTAACGGGG ATGAATCTGA GAAGAAAATG 
GACACTATGA GACTGCGAAT CAAACAGATG ATGGACGTCT CCAGGATTAA AGCCACTCTC 
AGCGTTGTGG AGGACGAGAG GAATCGCTAT ATGGAAAATC TCCTCGCTGA ACAGAAATCC 
AGACAAGAGC TGGAAGAGCA ATATCAGAAG GTCATGCATG ACCAGATGAA TCTGAACAAT 
GAGAAAACGC ATCTGGAGAA CCAGATGAAG AACCTGCAGC AGAGACTGGA GATCACCACT 
GAACTCTACC AGCAGAAGGA GAACGCACTG CAACAAAAGT TGACTCAGGA GGAGCTGGAG 
AGACGCGAGA AGGAGACGAA GCTGTCGGAG GTGGACAGTA AAGCGCTGCG CTCGGAGGAG 
GAGGTTCGAG CTCTCAAGCA GAAGATAAAG GACATCGAGG AGGAGATGCA GCAGAACGAA 
CGCTCACTCA AATCTGAGGT GGCAGTGCAA GAGAAGAAGG CCCATGAGAA CTGGTTAAAA 
GCACGCGCCT CAGAGAGAAC TCTGGTGGAG GAGAGAAGAG AATCAGCCAC TCTTCGGCAG 
AAGCTTGTGG AATACCGAGA TAAAATATCC GACATGGAAC AAAGTCTGTT TAAGTTGAAC 
TCTGGGCCGC CAGATCGACA CATGCCGCCG CCACAGCGCA GAGGGGATTC GTACGGTCCA 
TCTCCTGTGA GCGGCGGGGC TCCGTCTCCT CCTTTGATGA TCGAGGGTCC AGGACGGCCC 
CCATCAGCAC CTGTAGGGCG ACGCGGTGAG CCGTTCGGTC CACGACCCCC GTCTGATCCT 
CACGGACGCT TCTCTGAGCT TGGACACCCG CTGCCATCCA GACCAGAAAT GTTTCAGCCG 
ATGACATCAT CTCCATGTGC TCATGATGGG CCGATGCTGT CCAAATCTCA GAGTCAGGGA 
TCCTTCCTGC CCTCCCCGAT CCGAGATTCT CCGGTTCCTC CACCTAACGT CCCGCTGAAG 
CCTTACGGTC CCCCAGGGAT GCCGCCCAAT GGGCCACCGA TGATGATCCG ACCACCCAAC 
GGACACCTTC CCATGATGCC CCCCGACCCG CGCTTCAGAC CTCCACACAT GGACTCTTAC 
GGCCCGCCTC CACACATCGG CCCGTTTGGA CCCGTGCCTC CACCTTTTGC TCGAGGGCCT 
CCACTGGGGC CGCCACTCGG ACAGCGGGAC GTCCCGCCAG ATTTCTTTGC ACCCCGCGGC 
CTCCCTCCTC GGCCCTTTCC GCCTGGACCT CTGCCACCCC CCGGAGCCAT GATCCCTCCT 
CCATACAGCG GACACCGTTT TCCAGCAGCC CCTGCAGTAC TGTCTCAGAG CTCCAGAGAT 
GGAGACGAGG AGCCGCCGCA GACCCACGGG GAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160951.1
CDS	172..5730
Translation	MAAPNNPRFYSLFTLLLCSCFGGSCSDRRFSELKRCADEECSMLLGRGKAAKDFTGPDCRFLSFKKGETIYVYYKLSGQRSDVWAGSVGNHFGYFPKDYLNINHIYTEKELEVPTEETDFVCFETGLDKFESYDIDVLLGSKLFMEIENSTEESKEADPYDISETEESVEHLDSESLLESEAVHSESRLPLQSELIDSESVLILQQESVDSESKLPLELESVNLESASTHKSVNSETESTHESEFVYSKPALTPESESVDSESRTKSIDSESTTQLTNSESTTQSTDSESRTQSTDSESTKQSTNSESRTQSTDSESMLKSTDSELTTQSTDLESTDSESTAESTDSESTTQSNDLESTSQSTDSESTAESNDSESATRSNDSGSTLQSNDSESATQSNDSGSTLQSNASESTSQSADSESTTQPIDSESTLALESESFESELKSLPLESESFDSESTLPLKSESVDSESVEASESDTLIPKSTEDDSDSSQPKTVVSSESTLEPKEAATEETDTLTSQDDVETQPSDFDALNIDDVELLHIQHDSDSESQLETQTAGLDVDIEEPASHKDLPLQTLEKTLEDEDSWSSALNEKSESKETESFHEHAKESLLVHDLKAQRDFEEEENIDDVEFSHSPHNTKSASDESEVVPSEPALETLALNISEITENTKENADDFVMKASMNTNENAHKNIADDARDAAINRSQQPTESIDEDETENVSESPESAPLLYKTDEQIEKVKNELEDLLKNTLETEEDSPGGEEDEAEELLEDENALLSSNSPHLTDQNPEDNEQHDDDGLVESQQSENTTQADETETEEVGLPPEEPEYSDSVLRLTILRDHLKDEDTERMQKYFGLKNMFRIEAMFSDLDLEMKSAREMKTDTEEIEQTLDQITEASENSILDATEDILNDRDRKALEDGLQKQPEMYDVEAAMLDAFQEIVFALRQKYSTASDSVPLVEEEQSAYERDDPRADAEEVNNLDITTEITEPAETPEMHKEQNESEPILNPELNVNPDLSHNKDVDLEEDGGHFNRNKDAQISFEDAEEIQKGPHAILEKPVDIGFHFEMDPSSGSLESPSVSDFHDAETGSDSSDSSTSDELQDLVVLVKENLGVYGEILITALPEEWRPGPNFHGVPWEPVVVTVAIGALTILMFFWRTVLAIKGRSYQLTEKQLRDKIQQLLNEKSDAANKITELNDMIKEREQRLQNSEKSLSSSQHEIKGLKNHHQKMQSQWEQMSGSISQLNQTIVDTQEENANLNEKISKMHQRIEKYQKTLKNYDDERAKVHVLMDEAKLREDALKAQVLSFEKENGALKEQKKSLLGDAKDWQEKHKKLSEEIRVYHKSQKELEDSLVHKENEIDVLSSCIAELNRLGGCDSAELQKEDARMSNGDESEKKMDTMRLRIKQMMDVSRIKATLSVVEDERNRYMENLLAEQKSRQELEEQYQKVMHDQMNLNNEKTHLENQMKNLQQRLEITTELYQQKENALQQKLTQEELERREKETKLSEVDSKALRSEEEVRALKQKIKDIEEEMQQNERSLKSEVAVQEKKAHENWLKARASERTLVEERRESATLRQKLVEYRDKISDMEQSLFKLNSGPPDRHMPPPQRRGDSYGPSPVSGGAPSPPLMIEGPGRPPSAPVGRRGEPFGPRPPSDPHGRFSELGHPLPSRPEMFQPMTSSPCAHDGPMLSKSQSQGSFLPSPIRDSPVPPPNVPLKPYGPPGMPPNGPPMMIRPPNGHLPMMPPDPRFRPPHMDSYGPPPHIGPFGPVPPPFARGPPLGPPLGQRDVPPDFFAPRGLPPRPFPPGPLPPPGAMIPPPYSGHRFPAAPAVLSQSSRDGDEEPPQTHGEP

Target ORF information:

RefSeq Version	XM_005160894.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio melanoma inhibitory activity family, member 3 (mia3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005160894.3

ORF Insert Sequence:

ATGGCTGCAC CGAATAATCC CCGGTTTTAC TCACTTTTTA CCCTCCTCCT GTGCTCGTGC 
TTCGGCGGGA GCTGCTCGGA CCGGCGGTTC TCGGAGCTGA AGCGCTGCGC GGATGAAGAA 
TGCAGCATGC TTCTCGGCAG AGGAAAGGCC GCAAAGGATT TCACGGGGCC GGACTGTCGG 
TTTCTCTCTT TTAAGAAAGG AGAGACGATA TATGTATATT ATAAACTCTC AGGGCAGAGG 
TCAGACGTGT GGGCTGGTAG TGTTGGAAAC CACTTTGGCT ATTTTCCAAA AGACTATCTT 
AATATAAATC ATATATATAC TGAAAAAGAA TTGGAGGTAC CTACAGAAGA AACAGATTTT 
GTTTGCTTTG AGACTGGGCT TGATAAATTT GAAAGTTATG ACATAGATGT GTTATTAGGA 
AGCAAATTAT TTATGGAAAT TGAGAATTCA ACAGAAGAAT CAAAAGAAGC AGATCCGTAT 
GACATTTCTG AAACAGAAGA ATCAGTGGAG CATCTTGATT CAGAATCTCT GCTTGAATCA 
GAGGCAGTTC ATTCAGAATC AAGATTGCCT CTTCAATCAG AGTTGATCGA TTCAGAATCA 
GTATTGATTC TCCAACAAGA GTCAGTTGAT TCTGAATCAA AATTGCCTCT TGAATTAGAA 
TCAGTCAATT TAGAATCAGC ATCGACTCAT AAGTCAGTTA ATTCAGAAAC AGAATCGACT 
CATGAATCAG AGTTTGTTTA TTCGAAACCT GCATTGACTC CTGAATCAGA GTCAGTTGAT 
TCAGAATCAA GAACAAAGTC AATTGATTCA GAATCAACAA CACAGTTAAC TAATTCAGAA 
TCAACAACAC AGTCAACTGA TTCAGAATCA AGAACACAGT CAACTGATTC AGAATCAACA 
AAACAGTCAA CTAATTCAGA ATCAAGAACA CAGTCAACTG ATTCAGAATC AATGTTAAAG 
TCAACCGATT CAGAATTAAC CACACAGTCA ACCGATTTAG AATCAACTGA TTCAGAATCA 
ACAGCAGAGT CAACTGATTC AGAATCAACA ACACAGTCGA ATGATTTAGA ATCAACATCA 
CAGTCAACGG ATTCAGAATC AACAGCAGAG TCAAATGATT CAGAATCAGC AACACGGTCA 
AATGATTCAG GATCAACATT ACAGTCAAAT GATTCAGAAT CAGCAACACA GTCAAATGAT 
TCAGGATCAA CATTACAGTC AAATGCTTCA GAATCAACGT CGCAGTCAGC AGATTCAGAA 
TCAACAACAC AGCCAATTGA TTCAGAATCA ACATTGGCTC TTGAATCAGA ATCATTTGAG 
TCAGAATTAA AATCATTACC TCTTGAGTCA GAGTCATTTG ATTCAGAATC AACACTGCCT 
CTTAAATCAG AGTCAGTAGA TTCAGAATCT GTAGAAGCTT CTGAATCAGA CACATTGATT 
CCTAAATCTA CTGAAGATGA CTCTGATAGC TCTCAGCCAA AGACCGTCGT CTCCTCAGAG 
TCAACACTGG AGCCTAAAGA AGCAGCGACA GAAGAGACAG ATACTTTAAC AAGCCAAGAT 
GATGTAGAAA CACAACCATC AGATTTTGAT GCACTAAACA TTGATGATGT TGAACTTTTG 
CACATCCAAC ATGACTCTGA TTCTGAGTCA CAGCTTGAAA CACAAACAGC AGGTTTAGAT 
GTAGATATCG AAGAGCCTGC TAGCCATAAA GATTTACCAC TACAAACTTT AGAGAAAACA 
CTTGAGGATG AGGACAGCTG GTCATCAGCA TTAAATGAAA AAAGTGAATC GAAAGAGACT 
GAATCTTTTC ACGAACACGC CAAAGAATCA CTTTTAGTTC ATGACCTCAA AGCACAAAGA 
GATTTTGAAG AAGAGGAAAA TATTGATGAT GTGGAATTTT CTCATTCTCC TCACAATACG 
AAATCTGCTT CAGATGAGAG TGAAGTCGTC CCATCTGAAC CAGCTCTGGA AACATTAGCA 
TTAAATATAA GTGAAATCAC AGAGAATACA AAGGAAAATG CAGATGATTT TGTTATGAAG 
GCCAGCATGA ACACCAATGA AAATGCACAC AAAAACATTG CAGACGATGC TCGGGATGCA 
GCGATAAACA GAAGCCAACA GCCTACAGAG TCCATCGATG AAGATGAAAC TGAGAACGTT 
TCTGAAAGCC CAGAATCTGC TCCTCTACTT TATAAAACAG ACGAACAGAT CGAAAAGGTG 
AAGAACGAAC TGGAGGATCT GCTTAAAAAC ACACTGGAGA CTGAGGAAGA TAGTCCTGGA 
GGTGAAGAGG ACGAGGCTGA AGAGCTTCTG GAAGACGAAA ACGCTCTTCT TTCATCTAAT 
AGTCCTCACC TCACAGACCA AAATCCTGAA GACAATGAGC AACATGATGA TGATGGTTTG 
GTTGAATCTC AACAAAGCGA AAACACAACA CAAGCAGATG AAACTGAAAC TGAAGAGGTT 
GGTCTTCCTC CTGAAGAGCC TGAATACAGC GATAGCGTAC TGAGACTCAC AATATTGCGA 
GATCACTTGA AGGATGAGGA TACCGAGCGC ATGCAGAAGT ACTTCGGCCT GAAGAACATG 
TTCAGAATCG AAGCCATGTT CTCTGATCTG GATCTTGAGA TGAAGTCTGC TCGGGAGATG 
AAGACGGATA CGGAGGAGAT CGAGCAAACG CTGGACCAGA TCACGGAGGC TTCAGAAAAC 
TCCATTCTCG ATGCCACAGA GGACATACTT AACGACAGAG ACCGGAAAGC TCTGGAGGAT 
GGACTGCAGA AGCAACCGGA GATGTATGAT GTTGAGGCCG CCATGTTGGA CGCTTTTCAG 
GAGATCGTTT TCGCTTTGCG ACAGAAATAT TCGACAGCCA GCGACAGCGT CCCATTGGTC 
GAGGAGGAAC AAAGTGCATA TGAAAGAGAT GACCCCCGAG CTGACGCTGA AGAGGTGAAC 
AATCTGGACA TTACAACTGA AATAACTGAG CCAGCTGAAA CTCCTGAAAT GCACAAGGAG 
CAGAATGAAT CTGAACCGAT TCTGAATCCT GAATTGAACG TGAACCCAGA TTTGTCCCAC 
AATAAAGATG TGGATCTTGA GGAGGACGGA GGCCATTTCA ACAGGAATAA AGACGCTCAA 
ATCAGTTTCG AAGACGCTGA GGAGATCCAA AAGGGACCTC ATGCTATTTT GGAGAAGCCA 
GTGGACATTG GCTTTCACTT TGAGATGGAT CCGTCTTCAG GTTCGTTGGA GTCTCCGAGT 
GTCTCCGATT TCCATGATGC AGAAACTGGC AGTGATTCCT CCGACTCATC GACTTCAGAT 
GAACTTCAGG ATTTGGTGGT GCTTGTCAAA GAAAATCTGG GAGTTTATGG AGAGATTCTG 
ATCACTGCGC TTCCAGAGGA ATGGCGACCG GGTCCCAATT TTCACGGAGT CCCCTGGGAG 
CCAGTGGTGG TCACAGTGGC CATCGGGGCC CTCACTATAC TGATGTTCTT CTGGAGGACG 
GTGCTCGCTA TTAAAGGCAG AAGCTATCAG TTAACTGAAA AGCAGCTTAG AGACAAAATC 
CAGCAGCTTC TCAATGAGAA ATCTGATGCT GCGAACAAGA TCACAGAATT AAATGACATG 
ATAAAAGAAA GAGAACAACG CCTGCAAAAC TCAGAGAAGT CGCTGAGTTC CAGCCAACAT 
GAAATAAAAG GGCTGAAGAA TCATCACCAG AAAATGCAGA GTCAGTGGGA GCAGATGTCT 
GGGAGTATTT CGCAGCTCAA TCAGACAATT GTGGACACAC AGGAAGAAAA CGCAAACCTT 
AATGAAAAGA TTTCCAAGAT GCACCAGAGA ATTGAGAAAT ACCAGAAAAC ACTGAAGAAT 
TACGATGATG AGCGTGCAAA GGTTCATGTC CTTATGGATG AAGCTAAACT CCGAGAAGAC 
GCTCTTAAGG CTCAGGTTCT GTCTTTTGAG AAGGAAAATG GTGCTTTAAA AGAGCAGAAG 
AAATCTCTCC TTGGCGACGC TAAAGACTGG CAGGAGAAGC ACAAGAAGCT GAGCGAGGAG 
ATCCGAGTTT ATCACAAATC CCAGAAAGAG CTGGAAGACT CTCTGGTGCA CAAGGAGAAC 
GAGATCGATG TTTTGTCCAG CTGTATCGCC GAGCTCAACC GACTGGGAGG CTGCGACTCT 
GCAGAGCTAC AGAAAGAAGA TGCTAGAATG TCTAACGGGG ATGAATCTGA GAAGAAAATG 
GACACTATGA GACTGCGAAT CAAACAGATG ATGGACGTCT CCAGGATTAA AGCCACTCTC 
AGCGTTGTGG AGGACGAGAG GAATCGCTAT ATGGAAAATC TCCTCGCTGA ACAGAAATCC 
AGACAAGAGC TGGAAGAGCA ATATCAGAAG GTCATGCATG ACCAGATGAA TCTGAACAAT 
GAGAAAACGC ATCTGGAGAA CCAGATGAAG AACCTGCAGC AGAGACTGGA GATCACCACT 
GAACTCTACC AGCAGAAGGA GAACGCACTG CAACAAAAGT TGACTCAGGA GGAGCTGGAG 
AGACGCGAGA AGGAGACGAA GCTGTCGGAG GTGGACAGTA AAGCGCTGCG CTCGGAGGAG 
GAGGTTCGAG CTCTCAAGCA GAAGATAAAG GACATCGAGG AGGAGATGCA GCAGAACGAA 
CGCTCACTCA AATCTGAGGT GGCAGTGCAA GAGAAGAAGG CCCATGAGAA CTGGTTAAAA 
GCACGCGCCT CAGAGAGAAC TCTGGTGGAG GAGAGAAGAG AATCAGCCAC TCTTCGGCAG 
AAGCTTGTGG AATACCGAGA TAAAATATCC GACATGGAAC AAAGTCTGTT TAAGTTGAAC 
TCTGGGCCGC CAGATCGACA CATGCCGCCG CCACAGCGCA GAGGGGATTC GTACGGTCCA 
TCTCCTGTGA GCGGCGGGGC TCCGTCTCCT CCTTTGATGA TCGAGGGTCC AGGACGGCCC 
CCATCAGCAC CTGTAGGGCG ACGCGGTGAG CCGTTCGGTC CACGACCCCC GTCTGATCCT 
CACGGACGCT TCTCTGAGCT TGGACACCCG CTGCCATCCA GACCAGAAAT GTTTCAGCCG 
ATGACATCAT CTCCATGTGC TCATGATGGG CCGATGCTGT CCAAATCTCA GAGTCAGGGA 
TCCTTCCTGC CCTCCCCGAT CCGAGATTCT CCGGTTCCTC CACCTAACGT CCCGCTGAAG 
CCTTACGGTC CCCCAGGGAT GCCGCCCAAT GGGCCACCGA TGATGATCCG ACCACCCAAC 
GGACACCTTC CCATGATGCC CCCCGACCCG CGCTTCAGAC CTCCACACAT GGACTCTTAC 
GGCCCGCCTC CACACATCGG CCCGTTTGGA CCCGTGCCTC CACCTTTTGC TCGAGGGCCT 
CCACTGGGGC CGCCACTCGG ACAGCGGGAC GTCCCGCCAG ATTTCTTTGC ACCCCGCGGC 
CTCCCTCCTC GGCCCTTTCC GCCTGGACCT CTGCCACCCC CCGGAGCCAT GATCCCTCCT 
CCATACAGCG GACACCGTTT TCCAGCAGCC CCTGCAGTAC TGTCTCAGAG CTCCAGAGAT 
GGAGACGAGG AGCCGCCGCA GACCCACGGG GAGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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mia3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

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