CDC42BPB cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	CDC42BPB
Entrez Gene ID	567039
Full Name	CDC42 binding protein kinase beta (DMPK-like)
Synonyms	si:dkeyp-93h6.2,wu:fb98c02,wu:fc48b05,wu:fc90d12
General protein information	Preferred Names CDC42 binding protein kinase beta (DMPK-like) Names serine/threonine-protein kinase MRCK beta
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001045125.2	NP_001038590.1	serine/threonine-protein kinase MRCK beta
XM_021468493.1	XP_021324168.1	serine/threonine-protein kinase MRCK beta isoform X2
XM_009294622.3	XP_009292897.1	serine/threonine-protein kinase MRCK beta isoform X3
XM_009294623.2	XP_009292898.1	serine/threonine-protein kinase MRCK beta isoform X2
XM_021468492.1	XP_021324167.1	serine/threonine-protein kinase MRCK beta isoform X1
XM_009294622.2	XP_009292897.1	serine/threonine-protein kinase MRCK beta isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CDC42BPB	XP_005623889.1
Bos taurus (cattle)	CDC42BPB	XP_003587632.2
Danio rerio (zebrafish)	cdc42bpb	NP_001038590.1
Xenopus tropicalis (tropical clawed frog)	cdc42bpb	XP_004917233.1
Homo sapiens (human)	CDC42BPB	NP_006026.3
Pan troglodytes (chimpanzee)	CDC42BPB	XP_510180.2
	LOC695720	XP_002805265.1
Mus musculus (house mouse)	Cdc42bpb	NP_898837.2
Rattus norvegicus (Norway rat)	Cdc42bpb	NP_446072.2
Gallus gallus (chicken)	CDC42BPB	XP_004936409.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The zebrafish gene map defines ancestral vertebrate chromosomes.
Woods IG, Wilson C, Friedlander B, Chang P, Reyes DK, Nix R, Kelly PD, Chu F, Postlethwait JH, Talbot WS
Genome research15(9)1307-14(2005 Sep)

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

Expression profiling and comparative genomics identify a conserved regulatory region controlling midline expression in the zebrafish embryo.
Dickmeis T, Plessy C, Rastegar S, Aanstad P, Herwig R, Chalmel F, Fischer N, Str?hle U
Genome research14(2)228-38(2004 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CDC42BPB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CDC42BPB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa150658	XM_021468492.1 Latest version!	Danio rerio CDC42 binding protein kinase beta (DMPK-like) (cdc42bpb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa150659	XM_021468493.1 Latest version!	Danio rerio CDC42 binding protein kinase beta (DMPK-like) (cdc42bpb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa36872	NM_001045125.2 Latest version!	Danio rerio CDC42 binding protein kinase beta (DMPK-like) (cdc42bpb), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa45054	XM_009294622.3 Latest version!	Danio rerio CDC42 binding protein kinase beta (DMPK-like) (cdc42bpb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa45055	XM_009294623.2 Latest version!	Danio rerio CDC42 binding protein kinase beta (DMPK-like) (cdc42bpb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa45054	XM_009294622.2	Danio rerio CDC42 binding protein kinase beta (DMPK-like) (cdc42bpb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CDC42BPB ( XM_021468492.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021324167.1 Danio rerio CDC42 binding protein kinase beta (DMPK-like) (cdc42bpb), transcript variant X1, mRNA.

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CloneID	ODa150658
Clone ID Related Accession (Same CDS sequence)	XM_021468492.1
Accession Version	XM_021468492.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase MRCK beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCGA AGGTGCGCCT GAAGAAGTTG GAGCAGTTGC TGCTGGATGG CCATCAGAAA 
AACGACAAGT CACTGAGTGT GGAGACACTG CTGGATATTC TCATATGTTT ATACAACGAG 
TGCAGCAACT CGCCCTTAAA ACGGGAAAAA CACGTGACGG ACTTTTTGGA ATGGGTGAAG 
CCGTTTACAA CCACTGTGAA AGAGATGCGC CTTCACAGAG ACGACTTCGA GATGCTGAAA 
GTGATCGGCA GGGGAGCCTT TGGAGAGGTT GCTGTGGTAA AGATGAAGCA CACAGAGCGG 
GTTTACGCCA TGAAGATCCT CAACAAGTGG GAGATGCTGA AAAGGGCTGA GACGGCATGC 
TTTCGGGAAG AGAGGAACGT CCTGGTGAAT GGAGACTGCC AGTGGATCAC TACCCTTCAT 
TATGCTTTTC AGGATGACAA CTATCTGTAC TTGGTGATGG ATTATTACGT TGGCGGTGAC 
CTGCTGACGC TGCTTAGTAA ATTTGAAGAC CGGCTGCCGG AGGACATGGC CAAGTTCTAC 
GTGGCGGAGA TGGTTCTCGC CATCCACTCC ATCCATCAGC AGCGCTACGT ACACAGGGAT 
ATAAAACCCG ACAACGTGCT GCTGGACATG AACGGCCACA TCCGACTCGC TGACTTCGGC 
TCCTGTCTGA AGATGATGCA AGACGGCACG GTCCAATCTT CAGTGGCTGT GGGCACACCA 
GACTACATCT CTCCAGAGAT CCTGCAGGCC ATGGAGGACG GCATGGGGAA ATACGGGCCG 
GAGTGTGACT GGTGGTCGCT GGGCGTTTGC ATGTATGAGA TGCTGTACGG CGAGACACCC 
TTTTACGCCG AATCCCTGGT GGAGACCTAT GGAAAGATCA TGAACCATGA GGAGCGTTTC 
CAGTTCCCTT CCCACATCAC GGATGTATCC GAGGATGCTA AAGACCTGAT CCAGAGGCTG 
ATTTGCAGCC GAGAGCGCCG TTTGGGTCAA CATGGCATCG AGGAGTTCAG GAAGCACGCC 
TTTTTCTCCG GCATCGACTG GGAGAACATC CGGAACATGG AGGCGCCATA CATCCCTGAC 
GTCAGCTCTC CATCAGACAC CTCCAACTTT GATGTAGATG ATGACGTACT GAAGAATCCA 
GACATTGCTC CTCCAGTTTC TCACACGGGT TTCACCGGGC AGCACTTGCC CTTTGTGGGC 
TTCACCTACA CTACAGAGAG CTGTTTCTCA GACGGCGGCA GCATGAGGGG TGTGGCGCTG 
TCTGACGGGG GCACCGGGGA GGATCTCCAG GATGGAGGCA TGCAGGTGGA GGCCTTTGAG 
AAGAGGATTC GCTGCCTGGA GCAAGAGAAA TTGGAGCTTA ACCGTAAGCT GCAAGAATCC 
ACTCAGGCTG TTCAATCTCT TCATGGCTCT GGTCGAGGAG CCGGCACTCT CGGACGAGAT 
AAAGAGATTA AAAAACTTAA TGAGGAAATC GATCGGCTTA AAAAGAAACT GGCAGACTCT 
GATAGGCTGG AGCACCAGTT GGAGGAAGCA GTGACTCTTC GACAGGACTT TGAAAGTTCC 
TCCACCAAAC TAAAAGCCCT GGACAAGCAA GTCAAAGCTC TCAAGCTGGA GAAAGAGGAT 
ATTCATAAGC AACTGGTGGA GTCTTTGGAC CGCCTGAAGT CTCAGACCAA GGAGCTGAAG 
GACGCGCACC AGCAGAGGAA GCTGGCCATG CAGGAGTTCT CGGAGGTGAA CGAGCGTATG 
GCCGAACTGC GCTCCCAGAA GCAGCGTCTG TCGCGCCAGC TGCGGGACAA GGAGGAGGAG 
ATGGAGGTGG TGATGCAGAA AATCGACGCC ATGCGCCAGG ACATCCGCAA AACCGAGAAG 
GCCCGCAAGG AGCTGGAGTC TCAGCTGGAG GATGCGCGTG CCGAAGCTTC TAAGGAGCGT 
AAACTTCGAG AGCACAGTGA GGTTTACTCC AAACAACTGG AGAGTGAACT GGAGACACTC 
AAGGTGAAGC AGGGCGCAGG TCGTGCGTCA GGCCTCGGTG CAGAGACTCA GCAGGAGCTC 
ACCAAACTCA AGTCTGAGCT AGATAAGAAA GGACTGTATT ATGAGGAAGA GCTGGTGAGA 
CGTGAAGCCA GTCACACTAC AGAGATGAAA AACCTGAGGA AGGAGCTCCA GGACTCTGAG 
GGTCAGCAGC TCTCCCTGCA CAAAGAGCTG CTTGCGCTTA AAGAAAAACT GGAAAAGGCC 
AAGAGAGAAC GGCAAATTGA GATGGAAGAG GCCATTAATG CCCTGAAAGA GCAGTATGAA 
CGTGAACGCA ACCTTATGAG TGAAGACAAC CGCAAACTCA CTGCAGAAAC AGACCGGCTC 
TGCAGTTTTG TGGACAAGCT GACCGCTCAG AACAGGCAAC TGGAGGACGA GCTCCAGGAA 
CTGGCATCAA AGAAGGAGAG CGTAGCACAC TGGGAAGCAC AGATCGCAGA GATTATCCAG 
TGGGTGAGCG ATGAGAAAGA CGCACGCGGC TACCTTCAGG CTCTGGCCTC CAAGATGACA 
GAAGAGTTGG AGTCTCTGCG CAGCTCCAGT TTGGGATCCA GAACACTGGA TCCGTTGTGG 
AAGGTGCGTC GCAGTCAAAA GCTGGATATG TCTGCCCGTC TAGAGCTGCA GTCGGCCTTG 
GAAGCTGAAA TCCGTGCCAA GCAACTGGTT CACGATGAAC TGCGCAAGGT CAAAGCTGCC 
AACATCTCTT TCGAGAGCAA ACTGAAGGAC ACCGAGGCCA AGAACAGAGA GTTGGAGGAA 
CAGCTGGAGA ACATGAAGAA GGAGATGGAG GAGAGCCGCT CACGATCAGA CAAAGGCCTG 
AAACTGCCAG ACTTCCAGGA CTCCATCTTT GACTACTTCA ATACCTCTCC TCTTGCCCAT 
GAGCTGACAT TCAGAGACTC TGTCTCCTCC TCATCTGCAT CTTCTCTGCT TGCATTTTGG 
GACGAAACCA GCTCTGTCGG AGATGTGGAC GTCGCACCTC AGAAGACTGA CATTCCCCCT 
TCCTCCCCAT CAGCAGCTAC AGAACAGGAT GAAGCAGTCA AGCCTCAAGC GACCACTCCT 
GCTACTCCTT CTCCAATGTA TCAGCCCACT CTCACTGCAC CAAAGCCCAA AGCCCATCAG 
CTGAGCATCA AGACCTTCTC CAGCCCGACT CAGTGCACTC ACTGTACTTC ACTGATGGTG 
GGCCAGATGC GTCAAGGATA CGCCTGCGAG GTATGCTCTT TCATCTGCCA CGTGTCCTGT 
AAAGACAACG CACCCCAGCT CTGCCCCATT CCTCCCGAGC AGACCAAGAG GCCTCTGGGT 
ATTGATGTGC AGAGGGGCAT TGGCACTGCG TACAAAGGTT TTGTCAGGAT CCCGAAGCCC 
ACCGGAGTGA AGAAGGGCTG GCAGAGAGCA TATGCTGTAG TGTGTGACTG TAAACTCTTT 
CTCTACGAGG TGCCAGAAGG GAAGTCCACT CAACCTGTCG TGATGGCCAG TCAGGTCCTT 
GACTTGAGGG ATGAGGAGTT CTCTGTTAGC TCTGTGTTGG CATCTGATGT GATCCATGCC 
AACCATAAAG ACGTTCCCTG TATATTCAGG GTAACCTCGT CGGCTCTGAA ATCCTCGATC 
CGGCCAGTTC CTTTGCTGAT TTTAGCTGAG AGCGAGGCGG AGAAGAGGAA GTGGGTGGGC 
ATCCTAGAGG GCCTGCAGAG CATCCTAGCC AAAAACCGGC TGAAGAACCG CGTGGTGCAT 
GTGCTGCACG AGGCCTACGA CAGCAGCCTA CCTGCCATCA AAACTACACT TTCTGCTGCC 
ATCGTAGATC GAGAACGAAT TGTCTTGGGG ACAGAAGATG GCCTTTTTGT AGTCGAGATC 
ACCAGAGATG TCATTGTTCG TGCCGCTGAC TGTAAAAAAG TCTGCCAGAT CGAGCTCATC 
CCCAAAGAGA AGATGGTGGC CCTGCTTTCT GGCCGTAATC GCCATGTCCA TTTATACCCC 
TGGGCGGCAC TAGAGGGCGC TGAAGGGGGT TTCGATGCCA AGCTGACAGA TACGAGAGGC 
TGCCAGGCCA TGACCATCGG TAACCTCCGC CCTGGCGGTC CAGCGTGTCT CTTTGCAGGC 
GTGAAGCGGC AGGTTTTCTG TTATGAAATC ACACGAGCGA AACCCCATCA TCGCAAACTC 
TGGGAAGTGC AGGCGCCAGG GGTCGTCCAA TGGCTGGGGA TAATTCGAGA ACGGATCTGC 
GTAGGTTACC CATCAGGCTT TGCTCTGCTG GCCATGCAGG GAGAATCGTC GCCGGTCAGT 
CTGGTGAGCC CAGGTGACCC CTCGCTGGCA TTTTTGGCCC AGCAGCCTCT GGATGCACTT 
CATGCTATGG AAGTGGGGTC CAATGAGCTG CTGCTGTGCT TCAGTCAGCT GGGAGTGTAC 
GTGGACGCGA CTGGAAGACG ATCACGAACT CAGGAGCTCA TGTGGCCCTC CACACCGCTC 
GCCTGCAGCT CAAACTCGAA CTGCCTGACG GTGTACAGCG ATTATGGGGT CGACGTGTTT 
GATGTTCACA CTATGGAATG GGTTCAGACC ATCTCATTAA GAAAAATTCG ACCTCTGAAT 
GTGGAAGGCA GTCTAAACCT TTTGAGCATT GAGCAACCCC ATCTCATCTA TTTCAGCAAC 
AACTCCTCGG ATGGCTGTGA CCTGGCCATC CCCGAGACCT CAGACAACAG CAAGAAGCTG 
ATGGTGAGGA CGCGCAGCAA GAGGAAGTTC CTTTTTAAAG TACCAGAGGA GGAGCGGCTG 
CAGCAGAGAA GGGAGATGCT GAGGGATCCT GAAATGAGAT CCAAGCTAAT CTCTCACCCC 
ACCAACTTCA ATCACGTTGC TCACATGGGC CCAGGCGACG GCATGCAGGT CCTCATGGAT 
CTGCCTCTGC CGAGCGAGAC TCCCTCCCCC TCATCCTCTC GCCACCATGC CCTGATCTCC 
CCTCCGACCA ACTTTGAGCA CGTCTATCAC ATGAGTCCCG TCTCTGCCGG GCTCTATCTG 
CAGAAAGAGC CTTCCTCCCA GCAGAGCCTC CTTCAGTTCT CCTCTTCCTC CTCCTCTCCT 
TCCACATCCT CCCTGGGAAG GAGTGTGATG CCCTCTTCTC AGGATGACCT GTCTAAAGAC 
AAACCTCGAC CTCTGTCTAG CATCTCACGC CAACAGAGGA GCAAGACCCA CATCACCCGT 
ACCGCCTCAG GAGGGGGAGA CTTCGGAGGA GCAGGGTCGT CCCGCAGTAT TTCTGATCAG 
GATCAAGACT ACGAGCGAGA GCCCGACTCA GACTCGACCA AGCATTCCAC CCCCTCGAAC 
AGCTCCAACC CCAGCAGCCC ACCGAGCCCC AACTCTCCCC ACCGCAGTCA GCTGACTCTG 
GACAGCCTGG ATCAGTCCCT GGATGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324167.1
CDS	634..6003
Translation	MSAKVRLKKLEQLLLDGHQKNDKSLSVETLLDILICLYNECSNSPLKREKHVTDFLEWVKPFTTTVKEMRLHRDDFEMLKVIGRGAFGEVAVVKMKHTERVYAMKILNKWEMLKRAETACFREERNVLVNGDCQWITTLHYAFQDDNYLYLVMDYYVGGDLLTLLSKFEDRLPEDMAKFYVAEMVLAIHSIHQQRYVHRDIKPDNVLLDMNGHIRLADFGSCLKMMQDGTVQSSVAVGTPDYISPEILQAMEDGMGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHEERFQFPSHITDVSEDAKDLIQRLICSRERRLGQHGIEEFRKHAFFSGIDWENIRNMEAPYIPDVSSPSDTSNFDVDDDVLKNPDIAPPVSHTGFTGQHLPFVGFTYTTESCFSDGGSMRGVALSDGGTGEDLQDGGMQVEAFEKRIRCLEQEKLELNRKLQESTQAVQSLHGSGRGAGTLGRDKEIKKLNEEIDRLKKKLADSDRLEHQLEEAVTLRQDFESSSTKLKALDKQVKALKLEKEDIHKQLVESLDRLKSQTKELKDAHQQRKLAMQEFSEVNERMAELRSQKQRLSRQLRDKEEEMEVVMQKIDAMRQDIRKTEKARKELESQLEDARAEASKERKLREHSEVYSKQLESELETLKVKQGAGRASGLGAETQQELTKLKSELDKKGLYYEEELVRREASHTTEMKNLRKELQDSEGQQLSLHKELLALKEKLEKAKRERQIEMEEAINALKEQYERERNLMSEDNRKLTAETDRLCSFVDKLTAQNRQLEDELQELASKKESVAHWEAQIAEIIQWVSDEKDARGYLQALASKMTEELESLRSSSLGSRTLDPLWKVRRSQKLDMSARLELQSALEAEIRAKQLVHDELRKVKAANISFESKLKDTEAKNRELEEQLENMKKEMEESRSRSDKGLKLPDFQDSIFDYFNTSPLAHELTFRDSVSSSSASSLLAFWDETSSVGDVDVAPQKTDIPPSSPSAATEQDEAVKPQATTPATPSPMYQPTLTAPKPKAHQLSIKTFSSPTQCTHCTSLMVGQMRQGYACEVCSFICHVSCKDNAPQLCPIPPEQTKRPLGIDVQRGIGTAYKGFVRIPKPTGVKKGWQRAYAVVCDCKLFLYEVPEGKSTQPVVMASQVLDLRDEEFSVSSVLASDVIHANHKDVPCIFRVTSSALKSSIRPVPLLILAESEAEKRKWVGILEGLQSILAKNRLKNRVVHVLHEAYDSSLPAIKTTLSAAIVDRERIVLGTEDGLFVVEITRDVIVRAADCKKVCQIELIPKEKMVALLSGRNRHVHLYPWAALEGAEGGFDAKLTDTRGCQAMTIGNLRPGGPACLFAGVKRQVFCYEITRAKPHHRKLWEVQAPGVVQWLGIIRERICVGYPSGFALLAMQGESSPVSLVSPGDPSLAFLAQQPLDALHAMEVGSNELLLCFSQLGVYVDATGRRSRTQELMWPSTPLACSSNSNCLTVYSDYGVDVFDVHTMEWVQTISLRKIRPLNVEGSLNLLSIEQPHLIYFSNNSSDGCDLAIPETSDNSKKLMVRTRSKRKFLFKVPEEERLQQRREMLRDPEMRSKLISHPTNFNHVAHMGPGDGMQVLMDLPLPSETPSPSSSRHHALISPPTNFEHVYHMSPVSAGLYLQKEPSSQQSLLQFSSSSSSPSTSSLGRSVMPSSQDDLSKDKPRPLSSISRQQRSKTHITRTASGGGDFGGAGSSRSISDQDQDYEREPDSDSTKHSTPSNSSNPSSPPSPNSPHRSQLTLDSLDQSLDG

Target ORF information:

RefSeq Version	XM_021468492.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio CDC42 binding protein kinase beta (DMPK-like) (cdc42bpb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468492.1

ORF Insert Sequence:

ATGTCTGCGA AGGTGCGCCT GAAGAAGTTG GAGCAGTTGC TGCTGGATGG CCATCAGAAA 
AACGACAAGT CACTGAGTGT GGAGACACTG CTGGATATTC TCATATGTTT ATACAACGAG 
TGCAGCAACT CGCCCTTAAA ACGGGAAAAA CACGTGACGG ACTTTTTGGA ATGGGTGAAG 
CCGTTTACAA CCACTGTGAA AGAGATGCGC CTTCACAGAG ACGACTTCGA GATGCTGAAA 
GTGATCGGCA GGGGAGCCTT TGGAGAGGTT GCTGTGGTAA AGATGAAGCA CACAGAGCGG 
GTTTACGCCA TGAAGATCCT CAACAAGTGG GAGATGCTGA AAAGGGCTGA GACGGCATGC 
TTTCGGGAAG AGAGGAACGT CCTGGTGAAT GGAGACTGCC AGTGGATCAC TACCCTTCAT 
TATGCTTTTC AGGATGACAA CTATCTGTAC TTGGTGATGG ATTATTACGT TGGCGGTGAC 
CTGCTGACGC TGCTTAGTAA ATTTGAAGAC CGGCTGCCGG AGGACATGGC CAAGTTCTAC 
GTGGCGGAGA TGGTTCTCGC CATCCACTCC ATCCATCAGC AGCGCTACGT ACACAGGGAT 
ATAAAACCCG ACAACGTGCT GCTGGACATG AACGGCCACA TCCGACTCGC TGACTTCGGC 
TCCTGTCTGA AGATGATGCA AGACGGCACG GTCCAATCTT CAGTGGCTGT GGGCACACCA 
GACTACATCT CTCCAGAGAT CCTGCAGGCC ATGGAGGACG GCATGGGGAA ATACGGGCCG 
GAGTGTGACT GGTGGTCGCT GGGCGTTTGC ATGTATGAGA TGCTGTACGG CGAGACACCC 
TTTTACGCCG AATCCCTGGT GGAGACCTAT GGAAAGATCA TGAACCATGA GGAGCGTTTC 
CAGTTCCCTT CCCACATCAC GGATGTATCC GAGGATGCTA AAGACCTGAT CCAGAGGCTG 
ATTTGCAGCC GAGAGCGCCG TTTGGGTCAA CATGGCATCG AGGAGTTCAG GAAGCACGCC 
TTTTTCTCCG GCATCGACTG GGAGAACATC CGGAACATGG AGGCGCCATA CATCCCTGAC 
GTCAGCTCTC CATCAGACAC CTCCAACTTT GATGTAGATG ATGACGTACT GAAGAATCCA 
GACATTGCTC CTCCAGTTTC TCACACGGGT TTCACCGGGC AGCACTTGCC CTTTGTGGGC 
TTCACCTACA CTACAGAGAG CTGTTTCTCA GACGGCGGCA GCATGAGGGG TGTGGCGCTG 
TCTGACGGGG GCACCGGGGA GGATCTCCAG GATGGAGGCA TGCAGGTGGA GGCCTTTGAG 
AAGAGGATTC GCTGCCTGGA GCAAGAGAAA TTGGAGCTTA ACCGTAAGCT GCAAGAATCC 
ACTCAGGCTG TTCAATCTCT TCATGGCTCT GGTCGAGGAG CCGGCACTCT CGGACGAGAT 
AAAGAGATTA AAAAACTTAA TGAGGAAATC GATCGGCTTA AAAAGAAACT GGCAGACTCT 
GATAGGCTGG AGCACCAGTT GGAGGAAGCA GTGACTCTTC GACAGGACTT TGAAAGTTCC 
TCCACCAAAC TAAAAGCCCT GGACAAGCAA GTCAAAGCTC TCAAGCTGGA GAAAGAGGAT 
ATTCATAAGC AACTGGTGGA GTCTTTGGAC CGCCTGAAGT CTCAGACCAA GGAGCTGAAG 
GACGCGCACC AGCAGAGGAA GCTGGCCATG CAGGAGTTCT CGGAGGTGAA CGAGCGTATG 
GCCGAACTGC GCTCCCAGAA GCAGCGTCTG TCGCGCCAGC TGCGGGACAA GGAGGAGGAG 
ATGGAGGTGG TGATGCAGAA AATCGACGCC ATGCGCCAGG ACATCCGCAA AACCGAGAAG 
GCCCGCAAGG AGCTGGAGTC TCAGCTGGAG GATGCGCGTG CCGAAGCTTC TAAGGAGCGT 
AAACTTCGAG AGCACAGTGA GGTTTACTCC AAACAACTGG AGAGTGAACT GGAGACACTC 
AAGGTGAAGC AGGGCGCAGG TCGTGCGTCA GGCCTCGGTG CAGAGACTCA GCAGGAGCTC 
ACCAAACTCA AGTCTGAGCT AGATAAGAAA GGACTGTATT ATGAGGAAGA GCTGGTGAGA 
CGTGAAGCCA GTCACACTAC AGAGATGAAA AACCTGAGGA AGGAGCTCCA GGACTCTGAG 
GGTCAGCAGC TCTCCCTGCA CAAAGAGCTG CTTGCGCTTA AAGAAAAACT GGAAAAGGCC 
AAGAGAGAAC GGCAAATTGA GATGGAAGAG GCCATTAATG CCCTGAAAGA GCAGTATGAA 
CGTGAACGCA ACCTTATGAG TGAAGACAAC CGCAAACTCA CTGCAGAAAC AGACCGGCTC 
TGCAGTTTTG TGGACAAGCT GACCGCTCAG AACAGGCAAC TGGAGGACGA GCTCCAGGAA 
CTGGCATCAA AGAAGGAGAG CGTAGCACAC TGGGAAGCAC AGATCGCAGA GATTATCCAG 
TGGGTGAGCG ATGAGAAAGA CGCACGCGGC TACCTTCAGG CTCTGGCCTC CAAGATGACA 
GAAGAGTTGG AGTCTCTGCG CAGCTCCAGT TTGGGATCCA GAACACTGGA TCCGTTGTGG 
AAGGTGCGTC GCAGTCAAAA GCTGGATATG TCTGCCCGTC TAGAGCTGCA GTCGGCCTTG 
GAAGCTGAAA TCCGTGCCAA GCAACTGGTT CACGATGAAC TGCGCAAGGT CAAAGCTGCC 
AACATCTCTT TCGAGAGCAA ACTGAAGGAC ACCGAGGCCA AGAACAGAGA GTTGGAGGAA 
CAGCTGGAGA ACATGAAGAA GGAGATGGAG GAGAGCCGCT CACGATCAGA CAAAGGCCTG 
AAACTGCCAG ACTTCCAGGA CTCCATCTTT GACTACTTCA ATACCTCTCC TCTTGCCCAT 
GAGCTGACAT TCAGAGACTC TGTCTCCTCC TCATCTGCAT CTTCTCTGCT TGCATTTTGG 
GACGAAACCA GCTCTGTCGG AGATGTGGAC GTCGCACCTC AGAAGACTGA CATTCCCCCT 
TCCTCCCCAT CAGCAGCTAC AGAACAGGAT GAAGCAGTCA AGCCTCAAGC GACCACTCCT 
GCTACTCCTT CTCCAATGTA TCAGCCCACT CTCACTGCAC CAAAGCCCAA AGCCCATCAG 
CTGAGCATCA AGACCTTCTC CAGCCCGACT CAGTGCACTC ACTGTACTTC ACTGATGGTG 
GGCCAGATGC GTCAAGGATA CGCCTGCGAG GTATGCTCTT TCATCTGCCA CGTGTCCTGT 
AAAGACAACG CACCCCAGCT CTGCCCCATT CCTCCCGAGC AGACCAAGAG GCCTCTGGGT 
ATTGATGTGC AGAGGGGCAT TGGCACTGCG TACAAAGGTT TTGTCAGGAT CCCGAAGCCC 
ACCGGAGTGA AGAAGGGCTG GCAGAGAGCA TATGCTGTAG TGTGTGACTG TAAACTCTTT 
CTCTACGAGG TGCCAGAAGG GAAGTCCACT CAACCTGTCG TGATGGCCAG TCAGGTCCTT 
GACTTGAGGG ATGAGGAGTT CTCTGTTAGC TCTGTGTTGG CATCTGATGT GATCCATGCC 
AACCATAAAG ACGTTCCCTG TATATTCAGG GTAACCTCGT CGGCTCTGAA ATCCTCGATC 
CGGCCAGTTC CTTTGCTGAT TTTAGCTGAG AGCGAGGCGG AGAAGAGGAA GTGGGTGGGC 
ATCCTAGAGG GCCTGCAGAG CATCCTAGCC AAAAACCGGC TGAAGAACCG CGTGGTGCAT 
GTGCTGCACG AGGCCTACGA CAGCAGCCTA CCTGCCATCA AAACTACACT TTCTGCTGCC 
ATCGTAGATC GAGAACGAAT TGTCTTGGGG ACAGAAGATG GCCTTTTTGT AGTCGAGATC 
ACCAGAGATG TCATTGTTCG TGCCGCTGAC TGTAAAAAAG TCTGCCAGAT CGAGCTCATC 
CCCAAAGAGA AGATGGTGGC CCTGCTTTCT GGCCGTAATC GCCATGTCCA TTTATACCCC 
TGGGCGGCAC TAGAGGGCGC TGAAGGGGGT TTCGATGCCA AGCTGACAGA TACGAGAGGC 
TGCCAGGCCA TGACCATCGG TAACCTCCGC CCTGGCGGTC CAGCGTGTCT CTTTGCAGGC 
GTGAAGCGGC AGGTTTTCTG TTATGAAATC ACACGAGCGA AACCCCATCA TCGCAAACTC 
TGGGAAGTGC AGGCGCCAGG GGTCGTCCAA TGGCTGGGGA TAATTCGAGA ACGGATCTGC 
GTAGGTTACC CATCAGGCTT TGCTCTGCTG GCCATGCAGG GAGAATCGTC GCCGGTCAGT 
CTGGTGAGCC CAGGTGACCC CTCGCTGGCA TTTTTGGCCC AGCAGCCTCT GGATGCACTT 
CATGCTATGG AAGTGGGGTC CAATGAGCTG CTGCTGTGCT TCAGTCAGCT GGGAGTGTAC 
GTGGACGCGA CTGGAAGACG ATCACGAACT CAGGAGCTCA TGTGGCCCTC CACACCGCTC 
GCCTGCAGCT CAAACTCGAA CTGCCTGACG GTGTACAGCG ATTATGGGGT CGACGTGTTT 
GATGTTCACA CTATGGAATG GGTTCAGACC ATCTCATTAA GAAAAATTCG ACCTCTGAAT 
GTGGAAGGCA GTCTAAACCT TTTGAGCATT GAGCAACCCC ATCTCATCTA TTTCAGCAAC 
AACTCCTCGG ATGGCTGTGA CCTGGCCATC CCCGAGACCT CAGACAACAG CAAGAAGCTG 
ATGGTGAGGA CGCGCAGCAA GAGGAAGTTC CTTTTTAAAG TACCAGAGGA GGAGCGGCTG 
CAGCAGAGAA GGGAGATGCT GAGGGATCCT GAAATGAGAT CCAAGCTAAT CTCTCACCCC 
ACCAACTTCA ATCACGTTGC TCACATGGGC CCAGGCGACG GCATGCAGGT CCTCATGGAT 
CTGCCTCTGC CGAGCGAGAC TCCCTCCCCC TCATCCTCTC GCCACCATGC CCTGATCTCC 
CCTCCGACCA ACTTTGAGCA CGTCTATCAC ATGAGTCCCG TCTCTGCCGG GCTCTATCTG 
CAGAAAGAGC CTTCCTCCCA GCAGAGCCTC CTTCAGTTCT CCTCTTCCTC CTCCTCTCCT 
TCCACATCCT CCCTGGGAAG GAGTGTGATG CCCTCTTCTC AGGATGACCT GTCTAAAGAC 
AAACCTCGAC CTCTGTCTAG CATCTCACGC CAACAGAGGA GCAAGACCCA CATCACCCGT 
ACCGCCTCAG GAGGGGGAGA CTTCGGAGGA GCAGGGTCGT CCCGCAGTAT TTCTGATCAG 
GATCAAGACT ACGAGCGAGA GCCCGACTCA GACTCGACCA AGCATTCCAC CCCCTCGAAC 
AGCTCCAACC CCAGCAGCCC ACCGAGCCCC AACTCTCCCC ACCGCAGTCA GCTGACTCTG 
GACAGCCTGG ATCAGTCCCT GGATGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPB ( XM_021468493.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021324168.1 Danio rerio CDC42 binding protein kinase beta (DMPK-like) (cdc42bpb), transcript variant X2, mRNA.

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CloneID	ODa150659
Clone ID Related Accession (Same CDS sequence)	XM_021468493.1
Accession Version	XM_021468493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5319bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase MRCK beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCGA AGGTGCGCCT GAAGAAGTTG GAGCAGTTGC TGCTGGATGG CCATCAGAAA 
AACGACAAGT CACTGAGTGT GGAGACACTG CTGGATATTC TCATATGTTT ATACAACGAG 
TGCAGCAACT CGCCCTTAAA ACGGGAAAAA CACGTGACGG ACTTTTTGGA ATGGGTGAAG 
CCGTTTACAA CCACTGTGAA AGAGATGCGC CTTCACAGAG ACGACTTCGA GATGCTGAAA 
GTGATCGGCA GGGGAGCCTT TGGAGAGGTT GCTGTGGTAA AGATGAAGCA CACAGAGCGG 
GTTTACGCCA TGAAGATCCT CAACAAGTGG GAGATGCTGA AAAGGGCTGA GACGGCATGC 
TTTCGGGAAG AGAGGAACGT CCTGGTGAAT GGAGACTGCC AGTGGATCAC TACCCTTCAT 
TATGCTTTTC AGGATGACAA CTATCTGTAC TTGGTGATGG ATTATTACGT TGGCGGTGAC 
CTGCTGACGC TGCTTAGTAA ATTTGAAGAC CGGCTGCCGG AGGACATGGC CAAGTTCTAC 
GTGGCGGAGA TGGTTCTCGC CATCCACTCC ATCCATCAGC AGCGCTACGT ACACAGGGAT 
ATAAAACCCG ACAACGTGCT GCTGGACATG AACGGCCACA TCCGACTCGC TGACTTCGGC 
TCCTGTCTGA AGATGATGCA AGACGGCACG GTCCAATCTT CAGTGGCTGT GGGCACACCA 
GACTACATCT CTCCAGAGAT CCTGCAGGCC ATGGAGGACG GCATGGGGAA ATACGGGCCG 
GAGTGTGACT GGTGGTCGCT GGGCGTTTGC ATGTATGAGA TGCTGTACGG CGAGACACCC 
TTTTACGCCG AATCCCTGGT GGAGACCTAT GGAAAGATCA TGAACCATGA GGAGCGTTTC 
CAGTTCCCTT CCCACATCAC GGATGTATCC GAGGATGCTA AAGACCTGAT CCAGAGGCTG 
ATTTGCAGCC GAGAGCGCCG TTTGGGTCAA CATGGCATCG AGGAGTTCAG GAAGCACGCC 
TTTTTCTCCG GCATCGACTG GGAGAACATC CGGAACATGG AGGCGCCATA CATCCCTGAC 
GTCAGCTCTC CATCAGACAC CTCCAACTTT GATGTAGATG ATGACGTACT GAAGAATCCA 
GACATTGCTC CTCCAGTTTC TCACACGGGT TTCACCGGGC AGCACTTGCC CTTTGTGGGC 
TTCACCTACA CTACAGAGAG CTGTTTCTCA GACGGCGGCA GCATGAGGGG TGTGGCGCTG 
TCTGACGGGG GCACCGGGGA GGATCTCCAG GATGGAGGCA TGCAGGTGGA GGCCTTTGAG 
AAGAGGATTC GCTGCCTGGA GCAAGAGAAA TTGGAGCTTA ACCGTAAGCT GCAAGAATCC 
ACTCAGGCTG TTCAATCTCT TCATGGCTCT GGTCGAGGAG CCGGCACTCT CGGACGAGAT 
AAAGAGATTA AAAAACTTAA TGAGGAAATC GATCGGCTTA AAAAGAAACT GGCAGACTCT 
GATAGGCTGG AGCACCAGTT GGAGGAAGCA GTGACTCTTC GACAGGACTT TGAAAGTTCC 
TCCACCAAAC TAAAAGCCCT GGACAAGCAA GTCAAAGCTC TCAAGCTGGA GAAAGAGGAT 
ATTCATAAGC AACTGGTGGA GTCTTTGGAC CGCCTGAAGT CTCAGACCAA GGAGCTGAAG 
GACGCGCACC AGCAGAGGAA GCTGGCCATG CAGGAGTTCT CGGAGGTGAA CGAGCGTATG 
GCCGAACTGC GCTCCCAGAA GCAGCGTCTG TCGCGCCAGC TGCGGGACAA GGAGGAGGAG 
ATGGAGGTGG TGATGCAGAA AATCGACGCC ATGCGCCAGG ACATCCGCAA AACCGAGAAG 
GCCCGCAAGG AGCTGGAGTC TCAGCTGGAG GATGCGCGTG CCGAAGCTTC TAAGGAGCGT 
AAACTTCGAG AGCACAGTGA GGTTTACTCC AAACAACTGG AGAGTGAACT GGAGACACTC 
AAGGTGAAGC AGGGCGCAGG TCGTGCGTCA GGCCTCGGTG CAGAGACTCA GCAGGAGCTC 
ACCAAACTCA AGTCTGAGCT AGATAAGAAA GGACTGTATT ATGAGGAAGA GCTGGTGAGA 
CGTGAAGCCA GTCACACTAC AGAGATGAAA AACCTGAGGA AGGAGCTCCA GGACTCTGAG 
GGTCAGCAGC TCTCCCTGCA CAAAGAGCTG CTTGCGCTTA AAGAAAAACT GGAAAAGGCC 
AAGAGAGAAC GGCAAATTGA GATGGAAGAG GCCATTAATG CCCTGAAAGA GCAGTATGAA 
CGTGAACGCA ACCTTATGAG TGAAGACAAC CGCAAACTCA CTGCAGAAAC AGACCGGCTC 
TGCAGTTTTG TGGACAAGCT GACCGCTCAG AACAGGCAAC TGGAGGACGA GCTCCAGGAA 
CTGGCATCAA AGAAGGAGAG CGTAGCACAC TGGGAAGCAC AGATCGCAGA GATTATCCAG 
TGGGTGAGCG ATGAGAAAGA CGCACGCGGC TACCTTCAGG CTCTGGCCTC CAAGATGACA 
GAAGAGTTGG AGTCTCTGCG CAGCTCCAGT TTGGGATCCA GAACACTGGA TCCGTTGTGG 
AAGGTGCGTC GCAGTCAAAA GCTGGATATG TCTGCCCGTC TAGAGCTGCA GTCGGCCTTG 
GAAGCTGAAA TCCGTGCCAA GCAACTGGTT CACGATGAAC TGCGCAAGGT CAAAGCTGCC 
AACATCTCTT TCGAGAGCAA ACTGAAGGAC ACCGAGGCCA AGAACAGAGA GTTGGAGGAA 
CAGCTGGAGA ACATGAAGAA GGAGATGGAG GAGAGCCGCT CACGATCAGA CAAAGGCCTG 
AAACTGCCAG ACTTCCAGGA CTCCATCTTT GACTACTTCA ATACCTCTCC TCTTGCCCAT 
GAGCTGACAT TCAGAACCAG CTCTGTCGGA GATGTGGACG TCGCACCTCA GAAGACTGAC 
ATTCCCCCTT CCTCCCCATC AGCAGCTACA GAACAGGATG AAGCAGTCAA GCCTCAAGCG 
ACCACTCCTG CTACTCCTTC TCCAATGTAT CAGCCCACTC TCACTGCACC AAAGCCCAAA 
GCCCATCAGC TGAGCATCAA GACCTTCTCC AGCCCGACTC AGTGCACTCA CTGTACTTCA 
CTGATGGTGG GCCAGATGCG TCAAGGATAC GCCTGCGAGG TATGCTCTTT CATCTGCCAC 
GTGTCCTGTA AAGACAACGC ACCCCAGCTC TGCCCCATTC CTCCCGAGCA GACCAAGAGG 
CCTCTGGGTA TTGATGTGCA GAGGGGCATT GGCACTGCGT ACAAAGGTTT TGTCAGGATC 
CCGAAGCCCA CCGGAGTGAA GAAGGGCTGG CAGAGAGCAT ATGCTGTAGT GTGTGACTGT 
AAACTCTTTC TCTACGAGGT GCCAGAAGGG AAGTCCACTC AACCTGTCGT GATGGCCAGT 
CAGGTCCTTG ACTTGAGGGA TGAGGAGTTC TCTGTTAGCT CTGTGTTGGC ATCTGATGTG 
ATCCATGCCA ACCATAAAGA CGTTCCCTGT ATATTCAGGG TAACCTCGTC GGCTCTGAAA 
TCCTCGATCC GGCCAGTTCC TTTGCTGATT TTAGCTGAGA GCGAGGCGGA GAAGAGGAAG 
TGGGTGGGCA TCCTAGAGGG CCTGCAGAGC ATCCTAGCCA AAAACCGGCT GAAGAACCGC 
GTGGTGCATG TGCTGCACGA GGCCTACGAC AGCAGCCTAC CTGCCATCAA AACTACACTT 
TCTGCTGCCA TCGTAGATCG AGAACGAATT GTCTTGGGGA CAGAAGATGG CCTTTTTGTA 
GTCGAGATCA CCAGAGATGT CATTGTTCGT GCCGCTGACT GTAAAAAAGT CTGCCAGATC 
GAGCTCATCC CCAAAGAGAA GATGGTGGCC CTGCTTTCTG GCCGTAATCG CCATGTCCAT 
TTATACCCCT GGGCGGCACT AGAGGGCGCT GAAGGGGGTT TCGATGCCAA GCTGACAGAT 
ACGAGAGGCT GCCAGGCCAT GACCATCGGT AACCTCCGCC CTGGCGGTCC AGCGTGTCTC 
TTTGCAGGCG TGAAGCGGCA GGTTTTCTGT TATGAAATCA CACGAGCGAA ACCCCATCAT 
CGCAAACTCT GGGAAGTGCA GGCGCCAGGG GTCGTCCAAT GGCTGGGGAT AATTCGAGAA 
CGGATCTGCG TAGGTTACCC ATCAGGCTTT GCTCTGCTGG CCATGCAGGG AGAATCGTCG 
CCGGTCAGTC TGGTGAGCCC AGGTGACCCC TCGCTGGCAT TTTTGGCCCA GCAGCCTCTG 
GATGCACTTC ATGCTATGGA AGTGGGGTCC AATGAGCTGC TGCTGTGCTT CAGTCAGCTG 
GGAGTGTACG TGGACGCGAC TGGAAGACGA TCACGAACTC AGGAGCTCAT GTGGCCCTCC 
ACACCGCTCG CCTGCAGCTC AAACTCGAAC TGCCTGACGG TGTACAGCGA TTATGGGGTC 
GACGTGTTTG ATGTTCACAC TATGGAATGG GTTCAGACCA TCTCATTAAG AAAAATTCGA 
CCTCTGAATG TGGAAGGCAG TCTAAACCTT TTGAGCATTG AGCAACCCCA TCTCATCTAT 
TTCAGCAACA ACTCCTCGGA TGGCTGTGAC CTGGCCATCC CCGAGACCTC AGACAACAGC 
AAGAAGCTGA TGGTGAGGAC GCGCAGCAAG AGGAAGTTCC TTTTTAAAGT ACCAGAGGAG 
GAGCGGCTGC AGCAGAGAAG GGAGATGCTG AGGGATCCTG AAATGAGATC CAAGCTAATC 
TCTCACCCCA CCAACTTCAA TCACGTTGCT CACATGGGCC CAGGCGACGG CATGCAGGTC 
CTCATGGATC TGCCTCTGCC GAGCGAGACT CCCTCCCCCT CATCCTCTCG CCACCATGCC 
CTGATCTCCC CTCCGACCAA CTTTGAGCAC GTCTATCACA TGAGTCCCGT CTCTGCCGGG 
CTCTATCTGC AGAAAGAGCC TTCCTCCCAG CAGAGCCTCC TTCAGTTCTC CTCTTCCTCC 
TCCTCTCCTT CCACATCCTC CCTGGGAAGG AGTGTGATGC CCTCTTCTCA GGATGACCTG 
TCTAAAGACA AACCTCGACC TCTGTCTAGC ATCTCACGCC AACAGAGGAG CAAGACCCAC 
ATCACCCGTA CCGCCTCAGG AGGGGGAGAC TTCGGAGGAG CAGGGTCGTC CCGCAGTATT 
TCTGATCAGG ATCAAGACTA CGAGCGAGAG CCCGACTCAG ACTCGACCAA GCATTCCACC 
CCCTCGAACA GCTCCAACCC CAGCAGCCCA CCGAGCCCCA ACTCTCCCCA CCGCAGTCAG 
CTGACTCTGG ACAGCCTGGA TCAGTCCCTG GATGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324168.1
CDS	634..5952
Translation	MSAKVRLKKLEQLLLDGHQKNDKSLSVETLLDILICLYNECSNSPLKREKHVTDFLEWVKPFTTTVKEMRLHRDDFEMLKVIGRGAFGEVAVVKMKHTERVYAMKILNKWEMLKRAETACFREERNVLVNGDCQWITTLHYAFQDDNYLYLVMDYYVGGDLLTLLSKFEDRLPEDMAKFYVAEMVLAIHSIHQQRYVHRDIKPDNVLLDMNGHIRLADFGSCLKMMQDGTVQSSVAVGTPDYISPEILQAMEDGMGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHEERFQFPSHITDVSEDAKDLIQRLICSRERRLGQHGIEEFRKHAFFSGIDWENIRNMEAPYIPDVSSPSDTSNFDVDDDVLKNPDIAPPVSHTGFTGQHLPFVGFTYTTESCFSDGGSMRGVALSDGGTGEDLQDGGMQVEAFEKRIRCLEQEKLELNRKLQESTQAVQSLHGSGRGAGTLGRDKEIKKLNEEIDRLKKKLADSDRLEHQLEEAVTLRQDFESSSTKLKALDKQVKALKLEKEDIHKQLVESLDRLKSQTKELKDAHQQRKLAMQEFSEVNERMAELRSQKQRLSRQLRDKEEEMEVVMQKIDAMRQDIRKTEKARKELESQLEDARAEASKERKLREHSEVYSKQLESELETLKVKQGAGRASGLGAETQQELTKLKSELDKKGLYYEEELVRREASHTTEMKNLRKELQDSEGQQLSLHKELLALKEKLEKAKRERQIEMEEAINALKEQYERERNLMSEDNRKLTAETDRLCSFVDKLTAQNRQLEDELQELASKKESVAHWEAQIAEIIQWVSDEKDARGYLQALASKMTEELESLRSSSLGSRTLDPLWKVRRSQKLDMSARLELQSALEAEIRAKQLVHDELRKVKAANISFESKLKDTEAKNRELEEQLENMKKEMEESRSRSDKGLKLPDFQDSIFDYFNTSPLAHELTFRTSSVGDVDVAPQKTDIPPSSPSAATEQDEAVKPQATTPATPSPMYQPTLTAPKPKAHQLSIKTFSSPTQCTHCTSLMVGQMRQGYACEVCSFICHVSCKDNAPQLCPIPPEQTKRPLGIDVQRGIGTAYKGFVRIPKPTGVKKGWQRAYAVVCDCKLFLYEVPEGKSTQPVVMASQVLDLRDEEFSVSSVLASDVIHANHKDVPCIFRVTSSALKSSIRPVPLLILAESEAEKRKWVGILEGLQSILAKNRLKNRVVHVLHEAYDSSLPAIKTTLSAAIVDRERIVLGTEDGLFVVEITRDVIVRAADCKKVCQIELIPKEKMVALLSGRNRHVHLYPWAALEGAEGGFDAKLTDTRGCQAMTIGNLRPGGPACLFAGVKRQVFCYEITRAKPHHRKLWEVQAPGVVQWLGIIRERICVGYPSGFALLAMQGESSPVSLVSPGDPSLAFLAQQPLDALHAMEVGSNELLLCFSQLGVYVDATGRRSRTQELMWPSTPLACSSNSNCLTVYSDYGVDVFDVHTMEWVQTISLRKIRPLNVEGSLNLLSIEQPHLIYFSNNSSDGCDLAIPETSDNSKKLMVRTRSKRKFLFKVPEEERLQQRREMLRDPEMRSKLISHPTNFNHVAHMGPGDGMQVLMDLPLPSETPSPSSSRHHALISPPTNFEHVYHMSPVSAGLYLQKEPSSQQSLLQFSSSSSSPSTSSLGRSVMPSSQDDLSKDKPRPLSSISRQQRSKTHITRTASGGGDFGGAGSSRSISDQDQDYEREPDSDSTKHSTPSNSSNPSSPPSPNSPHRSQLTLDSLDQSLDG

Target ORF information:

RefSeq Version	XM_021468493.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio CDC42 binding protein kinase beta (DMPK-like) (cdc42bpb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468493.1

ORF Insert Sequence:

ATGTCTGCGA AGGTGCGCCT GAAGAAGTTG GAGCAGTTGC TGCTGGATGG CCATCAGAAA 
AACGACAAGT CACTGAGTGT GGAGACACTG CTGGATATTC TCATATGTTT ATACAACGAG 
TGCAGCAACT CGCCCTTAAA ACGGGAAAAA CACGTGACGG ACTTTTTGGA ATGGGTGAAG 
CCGTTTACAA CCACTGTGAA AGAGATGCGC CTTCACAGAG ACGACTTCGA GATGCTGAAA 
GTGATCGGCA GGGGAGCCTT TGGAGAGGTT GCTGTGGTAA AGATGAAGCA CACAGAGCGG 
GTTTACGCCA TGAAGATCCT CAACAAGTGG GAGATGCTGA AAAGGGCTGA GACGGCATGC 
TTTCGGGAAG AGAGGAACGT CCTGGTGAAT GGAGACTGCC AGTGGATCAC TACCCTTCAT 
TATGCTTTTC AGGATGACAA CTATCTGTAC TTGGTGATGG ATTATTACGT TGGCGGTGAC 
CTGCTGACGC TGCTTAGTAA ATTTGAAGAC CGGCTGCCGG AGGACATGGC CAAGTTCTAC 
GTGGCGGAGA TGGTTCTCGC CATCCACTCC ATCCATCAGC AGCGCTACGT ACACAGGGAT 
ATAAAACCCG ACAACGTGCT GCTGGACATG AACGGCCACA TCCGACTCGC TGACTTCGGC 
TCCTGTCTGA AGATGATGCA AGACGGCACG GTCCAATCTT CAGTGGCTGT GGGCACACCA 
GACTACATCT CTCCAGAGAT CCTGCAGGCC ATGGAGGACG GCATGGGGAA ATACGGGCCG 
GAGTGTGACT GGTGGTCGCT GGGCGTTTGC ATGTATGAGA TGCTGTACGG CGAGACACCC 
TTTTACGCCG AATCCCTGGT GGAGACCTAT GGAAAGATCA TGAACCATGA GGAGCGTTTC 
CAGTTCCCTT CCCACATCAC GGATGTATCC GAGGATGCTA AAGACCTGAT CCAGAGGCTG 
ATTTGCAGCC GAGAGCGCCG TTTGGGTCAA CATGGCATCG AGGAGTTCAG GAAGCACGCC 
TTTTTCTCCG GCATCGACTG GGAGAACATC CGGAACATGG AGGCGCCATA CATCCCTGAC 
GTCAGCTCTC CATCAGACAC CTCCAACTTT GATGTAGATG ATGACGTACT GAAGAATCCA 
GACATTGCTC CTCCAGTTTC TCACACGGGT TTCACCGGGC AGCACTTGCC CTTTGTGGGC 
TTCACCTACA CTACAGAGAG CTGTTTCTCA GACGGCGGCA GCATGAGGGG TGTGGCGCTG 
TCTGACGGGG GCACCGGGGA GGATCTCCAG GATGGAGGCA TGCAGGTGGA GGCCTTTGAG 
AAGAGGATTC GCTGCCTGGA GCAAGAGAAA TTGGAGCTTA ACCGTAAGCT GCAAGAATCC 
ACTCAGGCTG TTCAATCTCT TCATGGCTCT GGTCGAGGAG CCGGCACTCT CGGACGAGAT 
AAAGAGATTA AAAAACTTAA TGAGGAAATC GATCGGCTTA AAAAGAAACT GGCAGACTCT 
GATAGGCTGG AGCACCAGTT GGAGGAAGCA GTGACTCTTC GACAGGACTT TGAAAGTTCC 
TCCACCAAAC TAAAAGCCCT GGACAAGCAA GTCAAAGCTC TCAAGCTGGA GAAAGAGGAT 
ATTCATAAGC AACTGGTGGA GTCTTTGGAC CGCCTGAAGT CTCAGACCAA GGAGCTGAAG 
GACGCGCACC AGCAGAGGAA GCTGGCCATG CAGGAGTTCT CGGAGGTGAA CGAGCGTATG 
GCCGAACTGC GCTCCCAGAA GCAGCGTCTG TCGCGCCAGC TGCGGGACAA GGAGGAGGAG 
ATGGAGGTGG TGATGCAGAA AATCGACGCC ATGCGCCAGG ACATCCGCAA AACCGAGAAG 
GCCCGCAAGG AGCTGGAGTC TCAGCTGGAG GATGCGCGTG CCGAAGCTTC TAAGGAGCGT 
AAACTTCGAG AGCACAGTGA GGTTTACTCC AAACAACTGG AGAGTGAACT GGAGACACTC 
AAGGTGAAGC AGGGCGCAGG TCGTGCGTCA GGCCTCGGTG CAGAGACTCA GCAGGAGCTC 
ACCAAACTCA AGTCTGAGCT AGATAAGAAA GGACTGTATT ATGAGGAAGA GCTGGTGAGA 
CGTGAAGCCA GTCACACTAC AGAGATGAAA AACCTGAGGA AGGAGCTCCA GGACTCTGAG 
GGTCAGCAGC TCTCCCTGCA CAAAGAGCTG CTTGCGCTTA AAGAAAAACT GGAAAAGGCC 
AAGAGAGAAC GGCAAATTGA GATGGAAGAG GCCATTAATG CCCTGAAAGA GCAGTATGAA 
CGTGAACGCA ACCTTATGAG TGAAGACAAC CGCAAACTCA CTGCAGAAAC AGACCGGCTC 
TGCAGTTTTG TGGACAAGCT GACCGCTCAG AACAGGCAAC TGGAGGACGA GCTCCAGGAA 
CTGGCATCAA AGAAGGAGAG CGTAGCACAC TGGGAAGCAC AGATCGCAGA GATTATCCAG 
TGGGTGAGCG ATGAGAAAGA CGCACGCGGC TACCTTCAGG CTCTGGCCTC CAAGATGACA 
GAAGAGTTGG AGTCTCTGCG CAGCTCCAGT TTGGGATCCA GAACACTGGA TCCGTTGTGG 
AAGGTGCGTC GCAGTCAAAA GCTGGATATG TCTGCCCGTC TAGAGCTGCA GTCGGCCTTG 
GAAGCTGAAA TCCGTGCCAA GCAACTGGTT CACGATGAAC TGCGCAAGGT CAAAGCTGCC 
AACATCTCTT TCGAGAGCAA ACTGAAGGAC ACCGAGGCCA AGAACAGAGA GTTGGAGGAA 
CAGCTGGAGA ACATGAAGAA GGAGATGGAG GAGAGCCGCT CACGATCAGA CAAAGGCCTG 
AAACTGCCAG ACTTCCAGGA CTCCATCTTT GACTACTTCA ATACCTCTCC TCTTGCCCAT 
GAGCTGACAT TCAGAACCAG CTCTGTCGGA GATGTGGACG TCGCACCTCA GAAGACTGAC 
ATTCCCCCTT CCTCCCCATC AGCAGCTACA GAACAGGATG AAGCAGTCAA GCCTCAAGCG 
ACCACTCCTG CTACTCCTTC TCCAATGTAT CAGCCCACTC TCACTGCACC AAAGCCCAAA 
GCCCATCAGC TGAGCATCAA GACCTTCTCC AGCCCGACTC AGTGCACTCA CTGTACTTCA 
CTGATGGTGG GCCAGATGCG TCAAGGATAC GCCTGCGAGG TATGCTCTTT CATCTGCCAC 
GTGTCCTGTA AAGACAACGC ACCCCAGCTC TGCCCCATTC CTCCCGAGCA GACCAAGAGG 
CCTCTGGGTA TTGATGTGCA GAGGGGCATT GGCACTGCGT ACAAAGGTTT TGTCAGGATC 
CCGAAGCCCA CCGGAGTGAA GAAGGGCTGG CAGAGAGCAT ATGCTGTAGT GTGTGACTGT 
AAACTCTTTC TCTACGAGGT GCCAGAAGGG AAGTCCACTC AACCTGTCGT GATGGCCAGT 
CAGGTCCTTG ACTTGAGGGA TGAGGAGTTC TCTGTTAGCT CTGTGTTGGC ATCTGATGTG 
ATCCATGCCA ACCATAAAGA CGTTCCCTGT ATATTCAGGG TAACCTCGTC GGCTCTGAAA 
TCCTCGATCC GGCCAGTTCC TTTGCTGATT TTAGCTGAGA GCGAGGCGGA GAAGAGGAAG 
TGGGTGGGCA TCCTAGAGGG CCTGCAGAGC ATCCTAGCCA AAAACCGGCT GAAGAACCGC 
GTGGTGCATG TGCTGCACGA GGCCTACGAC AGCAGCCTAC CTGCCATCAA AACTACACTT 
TCTGCTGCCA TCGTAGATCG AGAACGAATT GTCTTGGGGA CAGAAGATGG CCTTTTTGTA 
GTCGAGATCA CCAGAGATGT CATTGTTCGT GCCGCTGACT GTAAAAAAGT CTGCCAGATC 
GAGCTCATCC CCAAAGAGAA GATGGTGGCC CTGCTTTCTG GCCGTAATCG CCATGTCCAT 
TTATACCCCT GGGCGGCACT AGAGGGCGCT GAAGGGGGTT TCGATGCCAA GCTGACAGAT 
ACGAGAGGCT GCCAGGCCAT GACCATCGGT AACCTCCGCC CTGGCGGTCC AGCGTGTCTC 
TTTGCAGGCG TGAAGCGGCA GGTTTTCTGT TATGAAATCA CACGAGCGAA ACCCCATCAT 
CGCAAACTCT GGGAAGTGCA GGCGCCAGGG GTCGTCCAAT GGCTGGGGAT AATTCGAGAA 
CGGATCTGCG TAGGTTACCC ATCAGGCTTT GCTCTGCTGG CCATGCAGGG AGAATCGTCG 
CCGGTCAGTC TGGTGAGCCC AGGTGACCCC TCGCTGGCAT TTTTGGCCCA GCAGCCTCTG 
GATGCACTTC ATGCTATGGA AGTGGGGTCC AATGAGCTGC TGCTGTGCTT CAGTCAGCTG 
GGAGTGTACG TGGACGCGAC TGGAAGACGA TCACGAACTC AGGAGCTCAT GTGGCCCTCC 
ACACCGCTCG CCTGCAGCTC AAACTCGAAC TGCCTGACGG TGTACAGCGA TTATGGGGTC 
GACGTGTTTG ATGTTCACAC TATGGAATGG GTTCAGACCA TCTCATTAAG AAAAATTCGA 
CCTCTGAATG TGGAAGGCAG TCTAAACCTT TTGAGCATTG AGCAACCCCA TCTCATCTAT 
TTCAGCAACA ACTCCTCGGA TGGCTGTGAC CTGGCCATCC CCGAGACCTC AGACAACAGC 
AAGAAGCTGA TGGTGAGGAC GCGCAGCAAG AGGAAGTTCC TTTTTAAAGT ACCAGAGGAG 
GAGCGGCTGC AGCAGAGAAG GGAGATGCTG AGGGATCCTG AAATGAGATC CAAGCTAATC 
TCTCACCCCA CCAACTTCAA TCACGTTGCT CACATGGGCC CAGGCGACGG CATGCAGGTC 
CTCATGGATC TGCCTCTGCC GAGCGAGACT CCCTCCCCCT CATCCTCTCG CCACCATGCC 
CTGATCTCCC CTCCGACCAA CTTTGAGCAC GTCTATCACA TGAGTCCCGT CTCTGCCGGG 
CTCTATCTGC AGAAAGAGCC TTCCTCCCAG CAGAGCCTCC TTCAGTTCTC CTCTTCCTCC 
TCCTCTCCTT CCACATCCTC CCTGGGAAGG AGTGTGATGC CCTCTTCTCA GGATGACCTG 
TCTAAAGACA AACCTCGACC TCTGTCTAGC ATCTCACGCC AACAGAGGAG CAAGACCCAC 
ATCACCCGTA CCGCCTCAGG AGGGGGAGAC TTCGGAGGAG CAGGGTCGTC CCGCAGTATT 
TCTGATCAGG ATCAAGACTA CGAGCGAGAG CCCGACTCAG ACTCGACCAA GCATTCCACC 
CCCTCGAACA GCTCCAACCC CAGCAGCCCA CCGAGCCCCA ACTCTCCCCA CCGCAGTCAG 
CTGACTCTGG ACAGCCTGGA TCAGTCCCTG GATGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPB ( NM_001045125.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001038590.1 Danio rerio CDC42 binding protein kinase beta (DMPK-like) (cdc42bpb), mRNA.

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CloneID	ODa36872
Clone ID Related Accession (Same CDS sequence)	NM_001045125.2
Accession Version	NM_001045125.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5127bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase MRCK beta
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC163554.1. On Jun 22, 2008 this sequence version replaced NM_001045125.1. ##Evidence-Data-START## Transcript exon combination :: BC163554.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCTGCGA AGGTGCGCCT GAAGAAGTTG GAGCAGTTGC TGCTGGATGG CCATCAGAAA 
AACGACAAGT CACTGAGTGT GGAGACACTG CTGGATATTC TCATATGTTT ATACAACGAG 
TGCAGCAACT CGCCCTTAAA ACGGGAAAAA CATGTGACGG ACTTTTTGGA ATGGGTGAAG 
CCGTTTACAA CCACTGTGAA AGAGATGCGC CTTCACAGAG ACGACTTCGA GATGCTGAAA 
GTGATCGGCA GGGGAGCCTT TGGAGAGGTT GCTGTGGTAA AGATGAAGCA CACAGAGCGG 
GTTTACGCCA TGAAGATCCT CAACAAGTGG GAGATGCTGA AAAGGGCTGA GACGGCATGC 
TTTCGGGAAG AGAGGAACGT CCTGGTGAAT GGAGACTGCC AGTGGATCAC TACCCTTCAT 
TATGCTTTTC AGGATGACAA CTATCTGTAC TTGGTGATGG ATTATTACGT TGGCGGTGAC 
CTGCTGACGC TGCTTAGTAA ATTTGAAGAC CGGCTGCCGG AGGACATGGC CAAGTTCTAC 
GTGGCGGAGA TGGTTCTCGC CATCCACTCC ATCCATCAGC AGCGCTACGT ACACAGGGAT 
ATAAAACCAG ACAACGTGCT GCTGGACATG AACGGCCACA TCCGACTCGC TGACTTCGGC 
TCCTGTCTGA AGATGATGCA AGACGGCACG GTCCAATCTT CAGTGGCTGT GGGCACACCA 
GACTACATCT CTCCAGAGAT CCTGCAGGCC ATGGAGGACG GCATGGGGAA ATACGGGCCG 
GAGTGTGACT GGTGGTCGCT GGGCGTTTGC ATGTATGAGA TGCTGTACGG CGAAACACCC 
TTTTACGCTG AATCCCTGGT GGAGACCTAT GGAAAGATCA TGAACCATGA GGAGCGTTTC 
CAGTTCCCTT CCCACATCAC GGATGTATCC GAGGATGCTA AAGACCTGAT CCAGAGGCTG 
ATTTGCAGCC GAGAGCGCCG TTTGGGTCAA CATGGCATCG AGGAGTTCAG GAAGCACGCC 
TTTTTCTCCG GCATCGACTG GGAGAACATC CGGAACATGG AGGCGCCATA CATCCCTGAC 
GTCAGCTCTC CATCAGACAC CTCCAACTTT GATGTAGATG ATGACGTGCT GAAAAATCCA 
GACATTGCTC CTCCAGTTTC TCACACGGGT TTCACCGGGC AGCACTTGCC CTTTGTGGGC 
TTCACCTACA CTACAGAGAG CTGTTTCTCA GACGGCGGCA GCATGAGGGG TGTGGCGCTG 
TCTGACGGGG GCACCGGGGA GGATCTCCAG GATGGAGGCA TGCAGGTTGA GGCCTTTGAG 
AAGAGGATTC GCTGCCTGGA GCAAGAGAAA TTGGAGCTTA ACCGTAAGCT GCAAGAATCC 
ACTCAGGCTG TTCAATCTCT TCATGGCTCT GGTCGAGGAG CTGGCACTCT CGGACGAGAT 
AAAGAGATTA AAAAACTTAA TGAGGAAATC GATCGGCTTA AAAAGAAACT GGCAGACTCT 
GATAGGCTGG AGCACCAGTT GGAGGAAGCA GTGACTCTTC GACAGGACTT TGAAAGTTCC 
TCCACCAAAC TAAAAGCCCT GGACAAGCAA GTCAAAGCTC TCAAGCTGGA GAAAGAGGAT 
ATTCATAAGC AACTGGTGGA GTCTTTGGAC CGCCTGAAGT CTCAGACCAA GGAGCTGAAG 
GACGCGCACC AGCAGAGGAA GCTGGCCATG CAGGAGTTCT CGGAGGTGAA CGAGCGTATG 
GCCGAACTGC GCTCCCAGAA GCAGCGTCTG TCGCGCCAGC TGCGGGACAA GGAGGAGGAG 
ATGGAGGTGG TGATGCAGAA AATCGACGCC ATGCGCCAGG ACATCCGCAA AACCGAGAAG 
GCCCGCAAGG AGCTGGAGTC TCAGCTGGAG GATGCGCGTG CCGAAGCTTC TAAGGAGCGT 
AAACTTCGAG AGCACAGTGA GGTTTACTCC AAACAACTGG AGAGTGAACT GGAGACACTC 
AAGGTGAAGC AGGGCGCAGG TCGTGCGTCA GGCCTCGGTG CAGAGACTCA GCAGGAGCTC 
ACCAAACTCA AGTCTGAGCT AGATAAGAAA GGACTGTATT ATGAGGAAGA GCTGGTGAGA 
CGTGAAGCCA GTCACACTAC AGAGATGAAA AACCTGAGGA AGGAGCTCCA GGACTCTGAG 
GGTCAGCAGC TCTCCCTGCA CAAAGAGCTG CTTGCGCTTA AAGAAAAACT GGAAAAGGCC 
AAGAGAGAAC GGCAAATTGA GATGGAAGAG GCCATTAATG CCCTGAAAGA GCAGTATGAA 
CGTGAACGCA ACCTTATGAG TGAAGACAAC CGCAAACTCA CTGCAGAAAC AGACCGGCTC 
TGCAGTTTTG TGGACAAGCT GACCGCTCAG AACAGGCAAC TGGAGGACGA GCTCCAGGAA 
CTGGCATCAA AGAAGGAGAG CGTAGCACAC TGGGAAGCAC AGATCGCAGA GATTATCCAG 
TGGGTGAGCG ATGAGAAAGA CGCACGCGGC TACCTTCAGG CTCTGGCCTC CAAGATGACA 
GAAGAGTTGG AGTCTCTGCG CAGCTCCAGT TTGGGATCCA GAACACTGGA TCCGTTGTGG 
AAGGTGCGTC GCAGTCAAAA GCTGGATATG TCTGCCCGTC TAGAGCTGCA GTCGGCCTTG 
GAAGCTGAAA TCCGTGCCAA GCAACTGGTT CACGATGAAC TGCGCAAGGT CAAAGCTGCC 
AACATCTCTT TCGAGAGCAA ACTGAAGGAC ACCGAGGCCA AGAACAGAGA GTTGGAGGAA 
CAGCTGGAGA ACATGAAGAA GGAGATGGAG GAGAGCCGCT CACGATCAGA CAAAGGCCTG 
AAACTGCCAG ACTTCCAGGA CTCCATCTTT GACTACTTCA ATACCTCTCC TCTTGCCCAT 
GAGCTGACAT TCAGAACCAG CTCTGTCGGA GATGTGGACG TCGCACCTCA GAAGACTGAC 
ATTCCCCCTT CCTCCCCATC AGCAGCTACA GAACAGGATG AAGCAGTCAA GCCTCAAGCG 
ACCACTCCTG CTACTCCTTC TCCAATGTAT CAGCCCACTC TCACTGCACC AAAGCCCAAA 
GCCCATCAGC TGAGCATCAA GACCTTCTCC AGCCCGACTC AGTGCACTCA CTGTACTTCA 
CTGATGGTGG GCCAGATGCG TCAAGGATAC GCCTGCGAGG TATGCTCTTT CATCTGCCAC 
GTGTCCTGTA AAGACAACGC ACCCCAGCTC TGCCCCATTC CTCCCGAGCA GACCAAGAGG 
CCTCTGGGTA TTGATGTGCA GAGGGGCATT GGCACTGCGT ACAAAGGTTT TGTCAGGATC 
CCGAAGCCCA CCGGAGTGAA GAAGGGCTGG CAGAGAGCAT ATGCTGTAGT GTGTGACTGT 
AAACTCTTTC TCTACGAGGT GCCAGAAGGG AAGTCCACTC AACCTGTCGT GATGGCCAGT 
CAGGTCCTTG ACTTGAGGGA TGAGGAGTTC TCTGTTAGCT CTGTGTTGGC ATCTGATGTG 
ATCCATGCCA ACCATAAAGA CGTTCCCTGT ATATTCAGGG TAACCTCGTC GGCTCTGAAA 
TCCTCGATCC GGCCAGTTCC TTTGCTGATT TTAGCTGAGA GCGAGGCGGA GAAGAGGAAG 
TGGGTGGGCA TCCTAGAGGG CCTGCAGAGC ATCCTAGCCA AAAACCGGCT GAAGAACCGC 
GTGGTGCATG TGCTGCACGA GGCCTACGAC AGCAGCCTAC CTGCCATCAA AACTACACTT 
TCTGCTGCCA TCGTAGATCG AGAACGAATT GTCTTGGGGA CAGAAGATGG CCTTTTTGTA 
GTCGAGATCA CCAGAGATGT CATTGTTCGT GCCGCTGACT GTAAAAAAGT CTGCCAGATC 
GAGCTCATCC CCAAAGAGAA GATGGTGGCC CTGCTTTCTG GCCGTAATCG CCATGTCCAT 
TTATACCCCT GGGCGGCACT AGAGGGCGCT GAAGGGGGTT TCGATGCCAA GCTGACAGAT 
ACGAGAGGCT GCCAGGCCAT GACCATCGGT AACCTCCGCC CTGGCGGTCC AGCGTGTCTC 
TTTGCAGGCG TGAAGCGGCA GGTTTTCTGT TATGAAATCA CACGAGCGAA ACCCCATCAT 
CGCAAACTCT GGGAAGTGCA GGCGCCAGGG GTCGTCCAAT GGCTGGGGAT AATTCGAGAA 
CGGATCTGCG TAGGTTACCC ATCAGGCTTT GCTCTGCTGG CCATGCAGGG AGAATCGTCG 
CCGGTCAGTC TGGTGAGCCC AGGTGACCCC TCGCTGGCAT TTTTGGCCCA GCAGCCTCTG 
GATGCACTTC ATGCTATGGA AGTGGGGTCC AATGAGCTGC TGCTGTGCTT CAGTCAGCTG 
GGAGTGTACG TGGACGCGAC TGGAAGACGA TCACGAACTC AGGAGCTCAT GTGGCCCTCC 
ACACCGCTCG CCTGCAGCTC AAACTCGAAC TGCCTGACGG TGTACAGCGA TTATGGGGTC 
GACGTGTTTG ATGTTCACAC TATGGAATGG GTTCAGACCA TCTCATTAAG AAAAATTCGA 
CCTCTGAATG TGGAAGGCAG TCTAAACCTT TTGAGCATTG AGCAACCCCA TCTCATCTAT 
TTCAGCAACA ACTCCTCGGA TGGCTGTGAC CTGGCCATCC CCGAGACCTC AGACAACAGC 
AAGAAGCTGA TGGTGAGGAC GCGCAGCAAG AGGAAGTTCC TTTTTAAAGT ACCAGAGGAG 
GAGCGGCTGC AGCAGAGAAG GGAGATGCTG AGGGATCCTG AAATGAGATC CAAGCTAATC 
TCTCACCCCA CCAACTTCAA TCACGTTGCT CACATGGGCC CAGGCGACGG CATGCAGGTC 
CTCATGGATC TGCCTCTGAG TGTGATGCCC TCTTCTCAGG ATGACCTGTC TAAAGACAAA 
CCTCGACCTC TGTCTAGCAT CTCACGCCAA CAGAGGAGCA AGACCCACAT CACCCGTACC 
GCCTCAGGAG GGGGAGACTT CGGAGGAGCA GGGTCGTCCC GCAGTATTTC TGATCAGGAT 
CAAGACTACG AGCGAGAGCC CGACTCAGAC TCGACCAAGC ATTCCACCCC CTCGAACAGC 
TCCAACCCCA GCAGCCCACC GAGCCCCAAC TCTCCCCACC GCAGTCAGCT GACTCTGGAC 
AGCCTGGATC AGTCCCTGGA TGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038590.1
CDS	23..5149
Translation	MSAKVRLKKLEQLLLDGHQKNDKSLSVETLLDILICLYNECSNSPLKREKHVTDFLEWVKPFTTTVKEMRLHRDDFEMLKVIGRGAFGEVAVVKMKHTERVYAMKILNKWEMLKRAETACFREERNVLVNGDCQWITTLHYAFQDDNYLYLVMDYYVGGDLLTLLSKFEDRLPEDMAKFYVAEMVLAIHSIHQQRYVHRDIKPDNVLLDMNGHIRLADFGSCLKMMQDGTVQSSVAVGTPDYISPEILQAMEDGMGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHEERFQFPSHITDVSEDAKDLIQRLICSRERRLGQHGIEEFRKHAFFSGIDWENIRNMEAPYIPDVSSPSDTSNFDVDDDVLKNPDIAPPVSHTGFTGQHLPFVGFTYTTESCFSDGGSMRGVALSDGGTGEDLQDGGMQVEAFEKRIRCLEQEKLELNRKLQESTQAVQSLHGSGRGAGTLGRDKEIKKLNEEIDRLKKKLADSDRLEHQLEEAVTLRQDFESSSTKLKALDKQVKALKLEKEDIHKQLVESLDRLKSQTKELKDAHQQRKLAMQEFSEVNERMAELRSQKQRLSRQLRDKEEEMEVVMQKIDAMRQDIRKTEKARKELESQLEDARAEASKERKLREHSEVYSKQLESELETLKVKQGAGRASGLGAETQQELTKLKSELDKKGLYYEEELVRREASHTTEMKNLRKELQDSEGQQLSLHKELLALKEKLEKAKRERQIEMEEAINALKEQYERERNLMSEDNRKLTAETDRLCSFVDKLTAQNRQLEDELQELASKKESVAHWEAQIAEIIQWVSDEKDARGYLQALASKMTEELESLRSSSLGSRTLDPLWKVRRSQKLDMSARLELQSALEAEIRAKQLVHDELRKVKAANISFESKLKDTEAKNRELEEQLENMKKEMEESRSRSDKGLKLPDFQDSIFDYFNTSPLAHELTFRTSSVGDVDVAPQKTDIPPSSPSAATEQDEAVKPQATTPATPSPMYQPTLTAPKPKAHQLSIKTFSSPTQCTHCTSLMVGQMRQGYACEVCSFICHVSCKDNAPQLCPIPPEQTKRPLGIDVQRGIGTAYKGFVRIPKPTGVKKGWQRAYAVVCDCKLFLYEVPEGKSTQPVVMASQVLDLRDEEFSVSSVLASDVIHANHKDVPCIFRVTSSALKSSIRPVPLLILAESEAEKRKWVGILEGLQSILAKNRLKNRVVHVLHEAYDSSLPAIKTTLSAAIVDRERIVLGTEDGLFVVEITRDVIVRAADCKKVCQIELIPKEKMVALLSGRNRHVHLYPWAALEGAEGGFDAKLTDTRGCQAMTIGNLRPGGPACLFAGVKRQVFCYEITRAKPHHRKLWEVQAPGVVQWLGIIRERICVGYPSGFALLAMQGESSPVSLVSPGDPSLAFLAQQPLDALHAMEVGSNELLLCFSQLGVYVDATGRRSRTQELMWPSTPLACSSNSNCLTVYSDYGVDVFDVHTMEWVQTISLRKIRPLNVEGSLNLLSIEQPHLIYFSNNSSDGCDLAIPETSDNSKKLMVRTRSKRKFLFKVPEEERLQQRREMLRDPEMRSKLISHPTNFNHVAHMGPGDGMQVLMDLPLSVMPSSQDDLSKDKPRPLSSISRQQRSKTHITRTASGGGDFGGAGSSRSISDQDQDYEREPDSDSTKHSTPSNSSNPSSPPSPNSPHRSQLTLDSLDQSLDG

Target ORF information:

RefSeq Version	NM_001045125.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio CDC42 binding protein kinase beta (DMPK-like) (cdc42bpb), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045125.2

ORF Insert Sequence:

ATGTCTGCGA AGGTGCGCCT GAAGAAGTTG GAGCAGTTGC TGCTGGATGG CCATCAGAAA 
AACGACAAGT CACTGAGTGT GGAGACACTG CTGGATATTC TCATATGTTT ATACAACGAG 
TGCAGCAACT CGCCCTTAAA ACGGGAAAAA CATGTGACGG ACTTTTTGGA ATGGGTGAAG 
CCGTTTACAA CCACTGTGAA AGAGATGCGC CTTCACAGAG ACGACTTCGA GATGCTGAAA 
GTGATCGGCA GGGGAGCCTT TGGAGAGGTT GCTGTGGTAA AGATGAAGCA CACAGAGCGG 
GTTTACGCCA TGAAGATCCT CAACAAGTGG GAGATGCTGA AAAGGGCTGA GACGGCATGC 
TTTCGGGAAG AGAGGAACGT CCTGGTGAAT GGAGACTGCC AGTGGATCAC TACCCTTCAT 
TATGCTTTTC AGGATGACAA CTATCTGTAC TTGGTGATGG ATTATTACGT TGGCGGTGAC 
CTGCTGACGC TGCTTAGTAA ATTTGAAGAC CGGCTGCCGG AGGACATGGC CAAGTTCTAC 
GTGGCGGAGA TGGTTCTCGC CATCCACTCC ATCCATCAGC AGCGCTACGT ACACAGGGAT 
ATAAAACCAG ACAACGTGCT GCTGGACATG AACGGCCACA TCCGACTCGC TGACTTCGGC 
TCCTGTCTGA AGATGATGCA AGACGGCACG GTCCAATCTT CAGTGGCTGT GGGCACACCA 
GACTACATCT CTCCAGAGAT CCTGCAGGCC ATGGAGGACG GCATGGGGAA ATACGGGCCG 
GAGTGTGACT GGTGGTCGCT GGGCGTTTGC ATGTATGAGA TGCTGTACGG CGAAACACCC 
TTTTACGCTG AATCCCTGGT GGAGACCTAT GGAAAGATCA TGAACCATGA GGAGCGTTTC 
CAGTTCCCTT CCCACATCAC GGATGTATCC GAGGATGCTA AAGACCTGAT CCAGAGGCTG 
ATTTGCAGCC GAGAGCGCCG TTTGGGTCAA CATGGCATCG AGGAGTTCAG GAAGCACGCC 
TTTTTCTCCG GCATCGACTG GGAGAACATC CGGAACATGG AGGCGCCATA CATCCCTGAC 
GTCAGCTCTC CATCAGACAC CTCCAACTTT GATGTAGATG ATGACGTGCT GAAAAATCCA 
GACATTGCTC CTCCAGTTTC TCACACGGGT TTCACCGGGC AGCACTTGCC CTTTGTGGGC 
TTCACCTACA CTACAGAGAG CTGTTTCTCA GACGGCGGCA GCATGAGGGG TGTGGCGCTG 
TCTGACGGGG GCACCGGGGA GGATCTCCAG GATGGAGGCA TGCAGGTTGA GGCCTTTGAG 
AAGAGGATTC GCTGCCTGGA GCAAGAGAAA TTGGAGCTTA ACCGTAAGCT GCAAGAATCC 
ACTCAGGCTG TTCAATCTCT TCATGGCTCT GGTCGAGGAG CTGGCACTCT CGGACGAGAT 
AAAGAGATTA AAAAACTTAA TGAGGAAATC GATCGGCTTA AAAAGAAACT GGCAGACTCT 
GATAGGCTGG AGCACCAGTT GGAGGAAGCA GTGACTCTTC GACAGGACTT TGAAAGTTCC 
TCCACCAAAC TAAAAGCCCT GGACAAGCAA GTCAAAGCTC TCAAGCTGGA GAAAGAGGAT 
ATTCATAAGC AACTGGTGGA GTCTTTGGAC CGCCTGAAGT CTCAGACCAA GGAGCTGAAG 
GACGCGCACC AGCAGAGGAA GCTGGCCATG CAGGAGTTCT CGGAGGTGAA CGAGCGTATG 
GCCGAACTGC GCTCCCAGAA GCAGCGTCTG TCGCGCCAGC TGCGGGACAA GGAGGAGGAG 
ATGGAGGTGG TGATGCAGAA AATCGACGCC ATGCGCCAGG ACATCCGCAA AACCGAGAAG 
GCCCGCAAGG AGCTGGAGTC TCAGCTGGAG GATGCGCGTG CCGAAGCTTC TAAGGAGCGT 
AAACTTCGAG AGCACAGTGA GGTTTACTCC AAACAACTGG AGAGTGAACT GGAGACACTC 
AAGGTGAAGC AGGGCGCAGG TCGTGCGTCA GGCCTCGGTG CAGAGACTCA GCAGGAGCTC 
ACCAAACTCA AGTCTGAGCT AGATAAGAAA GGACTGTATT ATGAGGAAGA GCTGGTGAGA 
CGTGAAGCCA GTCACACTAC AGAGATGAAA AACCTGAGGA AGGAGCTCCA GGACTCTGAG 
GGTCAGCAGC TCTCCCTGCA CAAAGAGCTG CTTGCGCTTA AAGAAAAACT GGAAAAGGCC 
AAGAGAGAAC GGCAAATTGA GATGGAAGAG GCCATTAATG CCCTGAAAGA GCAGTATGAA 
CGTGAACGCA ACCTTATGAG TGAAGACAAC CGCAAACTCA CTGCAGAAAC AGACCGGCTC 
TGCAGTTTTG TGGACAAGCT GACCGCTCAG AACAGGCAAC TGGAGGACGA GCTCCAGGAA 
CTGGCATCAA AGAAGGAGAG CGTAGCACAC TGGGAAGCAC AGATCGCAGA GATTATCCAG 
TGGGTGAGCG ATGAGAAAGA CGCACGCGGC TACCTTCAGG CTCTGGCCTC CAAGATGACA 
GAAGAGTTGG AGTCTCTGCG CAGCTCCAGT TTGGGATCCA GAACACTGGA TCCGTTGTGG 
AAGGTGCGTC GCAGTCAAAA GCTGGATATG TCTGCCCGTC TAGAGCTGCA GTCGGCCTTG 
GAAGCTGAAA TCCGTGCCAA GCAACTGGTT CACGATGAAC TGCGCAAGGT CAAAGCTGCC 
AACATCTCTT TCGAGAGCAA ACTGAAGGAC ACCGAGGCCA AGAACAGAGA GTTGGAGGAA 
CAGCTGGAGA ACATGAAGAA GGAGATGGAG GAGAGCCGCT CACGATCAGA CAAAGGCCTG 
AAACTGCCAG ACTTCCAGGA CTCCATCTTT GACTACTTCA ATACCTCTCC TCTTGCCCAT 
GAGCTGACAT TCAGAACCAG CTCTGTCGGA GATGTGGACG TCGCACCTCA GAAGACTGAC 
ATTCCCCCTT CCTCCCCATC AGCAGCTACA GAACAGGATG AAGCAGTCAA GCCTCAAGCG 
ACCACTCCTG CTACTCCTTC TCCAATGTAT CAGCCCACTC TCACTGCACC AAAGCCCAAA 
GCCCATCAGC TGAGCATCAA GACCTTCTCC AGCCCGACTC AGTGCACTCA CTGTACTTCA 
CTGATGGTGG GCCAGATGCG TCAAGGATAC GCCTGCGAGG TATGCTCTTT CATCTGCCAC 
GTGTCCTGTA AAGACAACGC ACCCCAGCTC TGCCCCATTC CTCCCGAGCA GACCAAGAGG 
CCTCTGGGTA TTGATGTGCA GAGGGGCATT GGCACTGCGT ACAAAGGTTT TGTCAGGATC 
CCGAAGCCCA CCGGAGTGAA GAAGGGCTGG CAGAGAGCAT ATGCTGTAGT GTGTGACTGT 
AAACTCTTTC TCTACGAGGT GCCAGAAGGG AAGTCCACTC AACCTGTCGT GATGGCCAGT 
CAGGTCCTTG ACTTGAGGGA TGAGGAGTTC TCTGTTAGCT CTGTGTTGGC ATCTGATGTG 
ATCCATGCCA ACCATAAAGA CGTTCCCTGT ATATTCAGGG TAACCTCGTC GGCTCTGAAA 
TCCTCGATCC GGCCAGTTCC TTTGCTGATT TTAGCTGAGA GCGAGGCGGA GAAGAGGAAG 
TGGGTGGGCA TCCTAGAGGG CCTGCAGAGC ATCCTAGCCA AAAACCGGCT GAAGAACCGC 
GTGGTGCATG TGCTGCACGA GGCCTACGAC AGCAGCCTAC CTGCCATCAA AACTACACTT 
TCTGCTGCCA TCGTAGATCG AGAACGAATT GTCTTGGGGA CAGAAGATGG CCTTTTTGTA 
GTCGAGATCA CCAGAGATGT CATTGTTCGT GCCGCTGACT GTAAAAAAGT CTGCCAGATC 
GAGCTCATCC CCAAAGAGAA GATGGTGGCC CTGCTTTCTG GCCGTAATCG CCATGTCCAT 
TTATACCCCT GGGCGGCACT AGAGGGCGCT GAAGGGGGTT TCGATGCCAA GCTGACAGAT 
ACGAGAGGCT GCCAGGCCAT GACCATCGGT AACCTCCGCC CTGGCGGTCC AGCGTGTCTC 
TTTGCAGGCG TGAAGCGGCA GGTTTTCTGT TATGAAATCA CACGAGCGAA ACCCCATCAT 
CGCAAACTCT GGGAAGTGCA GGCGCCAGGG GTCGTCCAAT GGCTGGGGAT AATTCGAGAA 
CGGATCTGCG TAGGTTACCC ATCAGGCTTT GCTCTGCTGG CCATGCAGGG AGAATCGTCG 
CCGGTCAGTC TGGTGAGCCC AGGTGACCCC TCGCTGGCAT TTTTGGCCCA GCAGCCTCTG 
GATGCACTTC ATGCTATGGA AGTGGGGTCC AATGAGCTGC TGCTGTGCTT CAGTCAGCTG 
GGAGTGTACG TGGACGCGAC TGGAAGACGA TCACGAACTC AGGAGCTCAT GTGGCCCTCC 
ACACCGCTCG CCTGCAGCTC AAACTCGAAC TGCCTGACGG TGTACAGCGA TTATGGGGTC 
GACGTGTTTG ATGTTCACAC TATGGAATGG GTTCAGACCA TCTCATTAAG AAAAATTCGA 
CCTCTGAATG TGGAAGGCAG TCTAAACCTT TTGAGCATTG AGCAACCCCA TCTCATCTAT 
TTCAGCAACA ACTCCTCGGA TGGCTGTGAC CTGGCCATCC CCGAGACCTC AGACAACAGC 
AAGAAGCTGA TGGTGAGGAC GCGCAGCAAG AGGAAGTTCC TTTTTAAAGT ACCAGAGGAG 
GAGCGGCTGC AGCAGAGAAG GGAGATGCTG AGGGATCCTG AAATGAGATC CAAGCTAATC 
TCTCACCCCA CCAACTTCAA TCACGTTGCT CACATGGGCC CAGGCGACGG CATGCAGGTC 
CTCATGGATC TGCCTCTGAG TGTGATGCCC TCTTCTCAGG ATGACCTGTC TAAAGACAAA 
CCTCGACCTC TGTCTAGCAT CTCACGCCAA CAGAGGAGCA AGACCCACAT CACCCGTACC 
GCCTCAGGAG GGGGAGACTT CGGAGGAGCA GGGTCGTCCC GCAGTATTTC TGATCAGGAT 
CAAGACTACG AGCGAGAGCC CGACTCAGAC TCGACCAAGC ATTCCACCCC CTCGAACAGC 
TCCAACCCCA GCAGCCCACC GAGCCCCAAC TCTCCCCACC GCAGTCAGCT GACTCTGGAC 
AGCCTGGATC AGTCCCTGGA TGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPB ( XM_009294622.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009292897.1 Danio rerio CDC42 binding protein kinase beta (DMPK-like) (cdc42bpb), transcript variant X3, mRNA.

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CloneID	ODa45054
Clone ID Related Accession (Same CDS sequence)	XM_009294622.3 , XM_009294622.2
Accession Version	XM_009294622.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase MRCK beta isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294622.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCGA AGGTGCGCCT GAAGAAGTTG GAGCAGTTGC TGCTGGATGG CCATCAGAAA 
AACGACAAGT CACTGAGTGT GGAGACACTG CTGGATATTC TCATATGTTT ATACAACGAG 
TGCAGCAACT CGCCCTTAAA ACGGGAAAAA CACGTGACGG ACTTTTTGGA ATGGGTGAAG 
CCGTTTACAA CCACTGTGAA AGAGATGCGC CTTCACAGAG ACGACTTCGA GATGCTGAAA 
GTGATCGGCA GGGGAGCCTT TGGAGAGGTT GCTGTGGTAA AGATGAAGCA CACAGAGCGG 
GTTTACGCCA TGAAGATCCT CAACAAGTGG GAGATGCTGA AAAGGGCTGA GACGGCATGC 
TTTCGGGAAG AGAGGAACGT CCTGGTGAAT GGAGACTGCC AGTGGATCAC TACCCTTCAT 
TATGCTTTTC AGGATGACAA CTATCTGTAC TTGGTGATGG ATTATTACGT TGGCGGTGAC 
CTGCTGACGC TGCTTAGTAA ATTTGAAGAC CGGCTGCCGG AGGACATGGC CAAGTTCTAC 
GTGGCGGAGA TGGTTCTCGC CATCCACTCC ATCCATCAGC AGCGCTACGT ACACAGGGAT 
ATAAAACCCG ACAACGTGCT GCTGGACATG AACGGCCACA TCCGACTCGC TGACTTCGGC 
TCCTGTCTGA AGATGATGCA AGACGGCACG GTCCAATCTT CAGTGGCTGT GGGCACACCA 
GACTACATCT CTCCAGAGAT CCTGCAGGCC ATGGAGGACG GCATGGGGAA ATACGGGCCG 
GAGTGTGACT GGTGGTCGCT GGGCGTTTGC ATGTATGAGA TGCTGTACGG CGAGACACCC 
TTTTACGCCG AATCCCTGGT GGAGACCTAT GGAAAGATCA TGAACCATGA GGAGCGTTTC 
CAGTTCCCTT CCCACATCAC GGATGTATCC GAGGATGCTA AAGACCTGAT CCAGAGGCTG 
ATTTGCAGCC GAGAGCGCCG TTTGGGTCAA CATGGCATCG AGGAGTTCAG GAAGCACGCC 
TTTTTCTCCG GCATCGACTG GGAGAACATC CGGAACATGG AGGCGCCATA CATCCCTGAC 
GTCAGCTCTC CATCAGACAC CTCCAACTTT GATGTAGATG ATGACGTACT GAAGAATCCA 
GACATTGCTC CTCCAGTTTC TCACACGGGT TTCACCGGGC AGCACTTGCC CTTTGTGGGC 
TTCACCTACA CTACAGAGAG CTGTTTCTCA GACGGCGGCA GCATGAGGGG TGTGGCGCTG 
TCTGACGGGG GCACCGGGGA GGATCTCCAG GATGGAGGCA TGCAGGTGGA GGCCTTTGAG 
AAGAGGATTC GCTGCCTGGA GCAAGAGAAA TTGGAGCTTA ACCGTAAGCT GCAAGAATCC 
ACTCAGGCTG TTCAATCTCT TCATGGCTCT GGTCGAGGAG CCGGCACTCT CGGACGAGAT 
AAAGAGATTA AAAAACTTAA TGAGGAAATC GATCGGCTTA AAAAGAAACT GGCAGACTCT 
GATAGGCTGG AGCACCAGTT GGAGGAAGCA GTGACTCTTC GACAGGACTT TGAAAGTTCC 
TCCACCAAAC TAAAAGCCCT GGACAAGCAA GTCAAAGCTC TCAAGCTGGA GAAAGAGGAT 
ATTCATAAGC AACTGGTGGA GTCTTTGGAC CGCCTGAAGT CTCAGACCAA GGAGCTGAAG 
GACGCGCACC AGCAGAGGAA GCTGGCCATG CAGGAGTTCT CGGAGGTGAA CGAGCGTATG 
GCCGAACTGC GCTCCCAGAA GCAGCGTCTG TCGCGCCAGC TGCGGGACAA GGAGGAGGAG 
ATGGAGGTGG TGATGCAGAA AATCGACGCC ATGCGCCAGG ACATCCGCAA AACCGAGAAG 
GCCCGCAAGG AGCTGGAGTC TCAGCTGGAG GATGCGCGTG CCGAAGCTTC TAAGGAGCGT 
AAACTTCGAG AGCACAGTGA GGTTTACTCC AAACAACTGG AGAGTGAACT GGAGACACTC 
AAGGTGAAGC AGGGCGCAGG TCGTGCGTCA GGCCTCGGTG CAGAGACTCA GCAGGAGCTC 
ACCAAACTCA AGTCTGAGCT AGATAAGAAA GGACTGTATT ATGAGGAAGA GCTGGTGAGA 
CGTGAAGCCA GTCACACTAC AGAGATGAAA AACCTGAGGA AGGAGCTCCA GGACTCTGAG 
GGTCAGCAGC TCTCCCTGCA CAAAGAGCTG CTTGCGCTTA AAGAAAAACT GGAAAAGGCC 
AAGAGAGAAC GGCAAATTGA GATGGAAGAG GCCATTAATG CCCTGAAAGA GCAGTATGAA 
CGTGAACGCA ACCTTATGAG TGAAGACAAC CGCAAACTCA CTGCAGAAAC AGACCGGCTC 
TGCAGTTTTG TGGACAAGCT GACCGCTCAG AACAGGCAAC TGGAGGACGA GCTCCAGGAA 
CTGGCATCAA AGAAGGAGAG CGTAGCACAC TGGGAAGCAC AGATCGCAGA GATTATCCAG 
TGGGTGAGCG ATGAGAAAGA CGCACGCGGC TACCTTCAGG CTCTGGCCTC CAAGATGACA 
GAAGAGTTGG AGTCTCTGCG CAGCTCCAGT TTGGGATCCA GAACACTGGA TCCGTTGTGG 
AAGGTGCGTC GCAGTCAAAA GCTGGATATG TCTGCCCGTC TAGAGCTGCA GTCGGCCTTG 
GAAGCTGAAA TCCGTGCCAA GCAACTGGTT CACGATGAAC TGCGCAAGGT CAAAGCTGCC 
AACATCTCTT TCGAGAGCAA ACTGAAGGAC ACCGAGGCCA AGAACAGAGA GTTGGAGGAA 
CAGCTGGAGA ACATGAAGAA GGAGATGGAG GAGAGCCGCT CACGATCAGA CAAAGGCCTG 
AAACTGCCAG ACTTCCAGGA CTCCATCTTT GACTACTTCA ATACCTCTCC TCTTGCCCAT 
GAGCTGACAT TCAGAGACTC TGTCTCCTCC TCATCTGCAT CTTCTCTGCT TGCATTTTGG 
GACGAAACCA GCTCTGTCGG AGATGTGGAC GTCGCACCTC AGAAGACTGA CATTCCCCCT 
TCCTCCCCAT CAGCAGCTAC AGAACAGGAT GAAGCAGTCA AGCCTCAAGC GACCACTCCT 
GCTACTCCTT CTCCAATGTA TCAGCCCACT CTCACTGCAC CAAAGCCCAA AGCCCATCAG 
CTGAGCATCA AGACCTTCTC CAGCCCGACT CAGTGCACTC ACTGTACTTC ACTGATGGTG 
GGCCAGATGC GTCAAGGATA CGCCTGCGAG GTATGCTCTT TCATCTGCCA CGTGTCCTGT 
AAAGACAACG CACCCCAGCT CTGCCCCATT CCTCCCGAGC AGACCAAGAG GCCTCTGGGT 
ATTGATGTGC AGAGGGGCAT TGGCACTGCG TACAAAGGTT TTGTCAGGAT CCCGAAGCCC 
ACCGGAGTGA AGAAGGGCTG GCAGAGAGCA TATGCTGTAG TGTGTGACTG TAAACTCTTT 
CTCTACGAGG TGCCAGAAGG GAAGTCCACT CAACCTGTCG TGATGGCCAG TCAGGTCCTT 
GACTTGAGGG ATGAGGAGTT CTCTGTTAGC TCTGTGTTGG CATCTGATGT GATCCATGCC 
AACCATAAAG ACGTTCCCTG TATATTCAGG GTAACCTCGT CGGCTCTGAA ATCCTCGATC 
CGGCCAGTTC CTTTGCTGAT TTTAGCTGAG AGCGAGGCGG AGAAGAGGAA GTGGGTGGGC 
ATCCTAGAGG GCCTGCAGAG CATCCTAGCC AAAAACCGGC TGAAGAACCG CGTGGTGCAT 
GTGCTGCACG AGGCCTACGA CAGCAGCCTA CCTGCCATCA AAACTACACT TTCTGCTGCC 
ATCGTAGATC GAGAACGAAT TGTCTTGGGG ACAGAAGATG GCCTTTTTGT AGTCGAGATC 
ACCAGAGATG TCATTGTTCG TGCCGCTGAC TGTAAAAAAG TCTGCCAGAT CGAGCTCATC 
CCCAAAGAGA AGATGGTGGC CCTGCTTTCT GGCCGTAATC GCCATGTCCA TTTATACCCC 
TGGGCGGCAC TAGAGGGCGC TGAAGGGGGT TTCGATGCCA AGCTGACAGA TACGAGAGGC 
TGCCAGGCCA TGACCATCGG TAACCTCCGC CCTGGCGGTC CAGCGTGTCT CTTTGCAGGC 
GTGAAGCGGC AGGTTTTCTG TTATGAAATC ACACGAGCGA AACCCCATCA TCGCAAACTC 
TGGGAAGTGC AGGCGCCAGG GGTCGTCCAA TGGCTGGGGA TAATTCGAGA ACGGATCTGC 
GTAGGTTACC CATCAGGCTT TGCTCTGCTG GCCATGCAGG GAGAATCGTC GCCGGTCAGT 
CTGGTGAGCC CAGGTGACCC CTCGCTGGCA TTTTTGGCCC AGCAGCCTCT GGATGCACTT 
CATGCTATGG AAGTGGGGTC CAATGAGCTG CTGCTGTGCT TCAGTCAGCT GGGAGTGTAC 
GTGGACGCGA CTGGAAGACG ATCACGAACT CAGGAGCTCA TGTGGCCCTC CACACCGCTC 
GCCTGCAGCT CAAACTCGAA CTGCCTGACG GTGTACAGCG ATTATGGGGT CGACGTGTTT 
GATGTTCACA CTATGGAATG GGTTCAGACC ATCTCATTAA GAAAAATTCG ACCTCTGAAT 
GTGGAAGGCA GTCTAAACCT TTTGAGCATT GAGCAACCCC ATCTCATCTA TTTCAGCAAC 
AACTCCTCGG ATGGCTGTGA CCTGGCCATC CCCGAGACCT CAGACAACAG CAAGAAGCTG 
ATGGTGAGGA CGCGCAGCAA GAGGAAGTTC CTTTTTAAAG TACCAGAGGA GGAGCGGCTG 
CAGCAGAGAA GGGAGATGCT GAGGGATCCT GAAATGAGAT CCAAGCTAAT CTCTCACCCC 
ACCAACTTCA ATCACGTTGC TCACATGGGC CCAGGCGACG GCATGCAGGT CCTCATGGAT 
CTGCCTCTGA GTGTGATGCC CTCTTCTCAG GATGACCTGT CTAAAGACAA ACCTCGACCT 
CTGTCTAGCA TCTCACGCCA ACAGAGGAGC AAGACCCACA TCACCCGTAC CGCCTCAGGA 
GGGGGAGACT TCGGAGGAGC AGGGTCGTCC CGCAGTATTT CTGATCAGGA TCAAGACTAC 
GAGCGAGAGC CCGACTCAGA CTCGACCAAG CATTCCACCC CCTCGAACAG CTCCAACCCC 
AGCAGCCCAC CGAGCCCCAA CTCTCCCCAC CGCAGTCAGC TGACTCTGGA CAGCCTGGAT 
CAGTCCCTGG ATGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292897.1
CDS	634..5811
Translation	MSAKVRLKKLEQLLLDGHQKNDKSLSVETLLDILICLYNECSNSPLKREKHVTDFLEWVKPFTTTVKEMRLHRDDFEMLKVIGRGAFGEVAVVKMKHTERVYAMKILNKWEMLKRAETACFREERNVLVNGDCQWITTLHYAFQDDNYLYLVMDYYVGGDLLTLLSKFEDRLPEDMAKFYVAEMVLAIHSIHQQRYVHRDIKPDNVLLDMNGHIRLADFGSCLKMMQDGTVQSSVAVGTPDYISPEILQAMEDGMGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHEERFQFPSHITDVSEDAKDLIQRLICSRERRLGQHGIEEFRKHAFFSGIDWENIRNMEAPYIPDVSSPSDTSNFDVDDDVLKNPDIAPPVSHTGFTGQHLPFVGFTYTTESCFSDGGSMRGVALSDGGTGEDLQDGGMQVEAFEKRIRCLEQEKLELNRKLQESTQAVQSLHGSGRGAGTLGRDKEIKKLNEEIDRLKKKLADSDRLEHQLEEAVTLRQDFESSSTKLKALDKQVKALKLEKEDIHKQLVESLDRLKSQTKELKDAHQQRKLAMQEFSEVNERMAELRSQKQRLSRQLRDKEEEMEVVMQKIDAMRQDIRKTEKARKELESQLEDARAEASKERKLREHSEVYSKQLESELETLKVKQGAGRASGLGAETQQELTKLKSELDKKGLYYEEELVRREASHTTEMKNLRKELQDSEGQQLSLHKELLALKEKLEKAKRERQIEMEEAINALKEQYERERNLMSEDNRKLTAETDRLCSFVDKLTAQNRQLEDELQELASKKESVAHWEAQIAEIIQWVSDEKDARGYLQALASKMTEELESLRSSSLGSRTLDPLWKVRRSQKLDMSARLELQSALEAEIRAKQLVHDELRKVKAANISFESKLKDTEAKNRELEEQLENMKKEMEESRSRSDKGLKLPDFQDSIFDYFNTSPLAHELTFRDSVSSSSASSLLAFWDETSSVGDVDVAPQKTDIPPSSPSAATEQDEAVKPQATTPATPSPMYQPTLTAPKPKAHQLSIKTFSSPTQCTHCTSLMVGQMRQGYACEVCSFICHVSCKDNAPQLCPIPPEQTKRPLGIDVQRGIGTAYKGFVRIPKPTGVKKGWQRAYAVVCDCKLFLYEVPEGKSTQPVVMASQVLDLRDEEFSVSSVLASDVIHANHKDVPCIFRVTSSALKSSIRPVPLLILAESEAEKRKWVGILEGLQSILAKNRLKNRVVHVLHEAYDSSLPAIKTTLSAAIVDRERIVLGTEDGLFVVEITRDVIVRAADCKKVCQIELIPKEKMVALLSGRNRHVHLYPWAALEGAEGGFDAKLTDTRGCQAMTIGNLRPGGPACLFAGVKRQVFCYEITRAKPHHRKLWEVQAPGVVQWLGIIRERICVGYPSGFALLAMQGESSPVSLVSPGDPSLAFLAQQPLDALHAMEVGSNELLLCFSQLGVYVDATGRRSRTQELMWPSTPLACSSNSNCLTVYSDYGVDVFDVHTMEWVQTISLRKIRPLNVEGSLNLLSIEQPHLIYFSNNSSDGCDLAIPETSDNSKKLMVRTRSKRKFLFKVPEEERLQQRREMLRDPEMRSKLISHPTNFNHVAHMGPGDGMQVLMDLPLSVMPSSQDDLSKDKPRPLSSISRQQRSKTHITRTASGGGDFGGAGSSRSISDQDQDYEREPDSDSTKHSTPSNSSNPSSPPSPNSPHRSQLTLDSLDQSLDG

Target ORF information:

RefSeq Version	XM_009294622.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio CDC42 binding protein kinase beta (DMPK-like) (cdc42bpb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294622.3

ORF Insert Sequence:

ATGTCTGCGA AGGTGCGCCT GAAGAAGTTG GAGCAGTTGC TGCTGGATGG CCATCAGAAA 
AACGACAAGT CACTGAGTGT GGAGACACTG CTGGATATTC TCATATGTTT ATACAACGAG 
TGCAGCAACT CGCCCTTAAA ACGGGAAAAA CACGTGACGG ACTTTTTGGA ATGGGTGAAG 
CCGTTTACAA CCACTGTGAA AGAGATGCGC CTTCACAGAG ACGACTTCGA GATGCTGAAA 
GTGATCGGCA GGGGAGCCTT TGGAGAGGTT GCTGTGGTAA AGATGAAGCA CACAGAGCGG 
GTTTACGCCA TGAAGATCCT CAACAAGTGG GAGATGCTGA AAAGGGCTGA GACGGCATGC 
TTTCGGGAAG AGAGGAACGT CCTGGTGAAT GGAGACTGCC AGTGGATCAC TACCCTTCAT 
TATGCTTTTC AGGATGACAA CTATCTGTAC TTGGTGATGG ATTATTACGT TGGCGGTGAC 
CTGCTGACGC TGCTTAGTAA ATTTGAAGAC CGGCTGCCGG AGGACATGGC CAAGTTCTAC 
GTGGCGGAGA TGGTTCTCGC CATCCACTCC ATCCATCAGC AGCGCTACGT ACACAGGGAT 
ATAAAACCCG ACAACGTGCT GCTGGACATG AACGGCCACA TCCGACTCGC TGACTTCGGC 
TCCTGTCTGA AGATGATGCA AGACGGCACG GTCCAATCTT CAGTGGCTGT GGGCACACCA 
GACTACATCT CTCCAGAGAT CCTGCAGGCC ATGGAGGACG GCATGGGGAA ATACGGGCCG 
GAGTGTGACT GGTGGTCGCT GGGCGTTTGC ATGTATGAGA TGCTGTACGG CGAGACACCC 
TTTTACGCCG AATCCCTGGT GGAGACCTAT GGAAAGATCA TGAACCATGA GGAGCGTTTC 
CAGTTCCCTT CCCACATCAC GGATGTATCC GAGGATGCTA AAGACCTGAT CCAGAGGCTG 
ATTTGCAGCC GAGAGCGCCG TTTGGGTCAA CATGGCATCG AGGAGTTCAG GAAGCACGCC 
TTTTTCTCCG GCATCGACTG GGAGAACATC CGGAACATGG AGGCGCCATA CATCCCTGAC 
GTCAGCTCTC CATCAGACAC CTCCAACTTT GATGTAGATG ATGACGTACT GAAGAATCCA 
GACATTGCTC CTCCAGTTTC TCACACGGGT TTCACCGGGC AGCACTTGCC CTTTGTGGGC 
TTCACCTACA CTACAGAGAG CTGTTTCTCA GACGGCGGCA GCATGAGGGG TGTGGCGCTG 
TCTGACGGGG GCACCGGGGA GGATCTCCAG GATGGAGGCA TGCAGGTGGA GGCCTTTGAG 
AAGAGGATTC GCTGCCTGGA GCAAGAGAAA TTGGAGCTTA ACCGTAAGCT GCAAGAATCC 
ACTCAGGCTG TTCAATCTCT TCATGGCTCT GGTCGAGGAG CCGGCACTCT CGGACGAGAT 
AAAGAGATTA AAAAACTTAA TGAGGAAATC GATCGGCTTA AAAAGAAACT GGCAGACTCT 
GATAGGCTGG AGCACCAGTT GGAGGAAGCA GTGACTCTTC GACAGGACTT TGAAAGTTCC 
TCCACCAAAC TAAAAGCCCT GGACAAGCAA GTCAAAGCTC TCAAGCTGGA GAAAGAGGAT 
ATTCATAAGC AACTGGTGGA GTCTTTGGAC CGCCTGAAGT CTCAGACCAA GGAGCTGAAG 
GACGCGCACC AGCAGAGGAA GCTGGCCATG CAGGAGTTCT CGGAGGTGAA CGAGCGTATG 
GCCGAACTGC GCTCCCAGAA GCAGCGTCTG TCGCGCCAGC TGCGGGACAA GGAGGAGGAG 
ATGGAGGTGG TGATGCAGAA AATCGACGCC ATGCGCCAGG ACATCCGCAA AACCGAGAAG 
GCCCGCAAGG AGCTGGAGTC TCAGCTGGAG GATGCGCGTG CCGAAGCTTC TAAGGAGCGT 
AAACTTCGAG AGCACAGTGA GGTTTACTCC AAACAACTGG AGAGTGAACT GGAGACACTC 
AAGGTGAAGC AGGGCGCAGG TCGTGCGTCA GGCCTCGGTG CAGAGACTCA GCAGGAGCTC 
ACCAAACTCA AGTCTGAGCT AGATAAGAAA GGACTGTATT ATGAGGAAGA GCTGGTGAGA 
CGTGAAGCCA GTCACACTAC AGAGATGAAA AACCTGAGGA AGGAGCTCCA GGACTCTGAG 
GGTCAGCAGC TCTCCCTGCA CAAAGAGCTG CTTGCGCTTA AAGAAAAACT GGAAAAGGCC 
AAGAGAGAAC GGCAAATTGA GATGGAAGAG GCCATTAATG CCCTGAAAGA GCAGTATGAA 
CGTGAACGCA ACCTTATGAG TGAAGACAAC CGCAAACTCA CTGCAGAAAC AGACCGGCTC 
TGCAGTTTTG TGGACAAGCT GACCGCTCAG AACAGGCAAC TGGAGGACGA GCTCCAGGAA 
CTGGCATCAA AGAAGGAGAG CGTAGCACAC TGGGAAGCAC AGATCGCAGA GATTATCCAG 
TGGGTGAGCG ATGAGAAAGA CGCACGCGGC TACCTTCAGG CTCTGGCCTC CAAGATGACA 
GAAGAGTTGG AGTCTCTGCG CAGCTCCAGT TTGGGATCCA GAACACTGGA TCCGTTGTGG 
AAGGTGCGTC GCAGTCAAAA GCTGGATATG TCTGCCCGTC TAGAGCTGCA GTCGGCCTTG 
GAAGCTGAAA TCCGTGCCAA GCAACTGGTT CACGATGAAC TGCGCAAGGT CAAAGCTGCC 
AACATCTCTT TCGAGAGCAA ACTGAAGGAC ACCGAGGCCA AGAACAGAGA GTTGGAGGAA 
CAGCTGGAGA ACATGAAGAA GGAGATGGAG GAGAGCCGCT CACGATCAGA CAAAGGCCTG 
AAACTGCCAG ACTTCCAGGA CTCCATCTTT GACTACTTCA ATACCTCTCC TCTTGCCCAT 
GAGCTGACAT TCAGAGACTC TGTCTCCTCC TCATCTGCAT CTTCTCTGCT TGCATTTTGG 
GACGAAACCA GCTCTGTCGG AGATGTGGAC GTCGCACCTC AGAAGACTGA CATTCCCCCT 
TCCTCCCCAT CAGCAGCTAC AGAACAGGAT GAAGCAGTCA AGCCTCAAGC GACCACTCCT 
GCTACTCCTT CTCCAATGTA TCAGCCCACT CTCACTGCAC CAAAGCCCAA AGCCCATCAG 
CTGAGCATCA AGACCTTCTC CAGCCCGACT CAGTGCACTC ACTGTACTTC ACTGATGGTG 
GGCCAGATGC GTCAAGGATA CGCCTGCGAG GTATGCTCTT TCATCTGCCA CGTGTCCTGT 
AAAGACAACG CACCCCAGCT CTGCCCCATT CCTCCCGAGC AGACCAAGAG GCCTCTGGGT 
ATTGATGTGC AGAGGGGCAT TGGCACTGCG TACAAAGGTT TTGTCAGGAT CCCGAAGCCC 
ACCGGAGTGA AGAAGGGCTG GCAGAGAGCA TATGCTGTAG TGTGTGACTG TAAACTCTTT 
CTCTACGAGG TGCCAGAAGG GAAGTCCACT CAACCTGTCG TGATGGCCAG TCAGGTCCTT 
GACTTGAGGG ATGAGGAGTT CTCTGTTAGC TCTGTGTTGG CATCTGATGT GATCCATGCC 
AACCATAAAG ACGTTCCCTG TATATTCAGG GTAACCTCGT CGGCTCTGAA ATCCTCGATC 
CGGCCAGTTC CTTTGCTGAT TTTAGCTGAG AGCGAGGCGG AGAAGAGGAA GTGGGTGGGC 
ATCCTAGAGG GCCTGCAGAG CATCCTAGCC AAAAACCGGC TGAAGAACCG CGTGGTGCAT 
GTGCTGCACG AGGCCTACGA CAGCAGCCTA CCTGCCATCA AAACTACACT TTCTGCTGCC 
ATCGTAGATC GAGAACGAAT TGTCTTGGGG ACAGAAGATG GCCTTTTTGT AGTCGAGATC 
ACCAGAGATG TCATTGTTCG TGCCGCTGAC TGTAAAAAAG TCTGCCAGAT CGAGCTCATC 
CCCAAAGAGA AGATGGTGGC CCTGCTTTCT GGCCGTAATC GCCATGTCCA TTTATACCCC 
TGGGCGGCAC TAGAGGGCGC TGAAGGGGGT TTCGATGCCA AGCTGACAGA TACGAGAGGC 
TGCCAGGCCA TGACCATCGG TAACCTCCGC CCTGGCGGTC CAGCGTGTCT CTTTGCAGGC 
GTGAAGCGGC AGGTTTTCTG TTATGAAATC ACACGAGCGA AACCCCATCA TCGCAAACTC 
TGGGAAGTGC AGGCGCCAGG GGTCGTCCAA TGGCTGGGGA TAATTCGAGA ACGGATCTGC 
GTAGGTTACC CATCAGGCTT TGCTCTGCTG GCCATGCAGG GAGAATCGTC GCCGGTCAGT 
CTGGTGAGCC CAGGTGACCC CTCGCTGGCA TTTTTGGCCC AGCAGCCTCT GGATGCACTT 
CATGCTATGG AAGTGGGGTC CAATGAGCTG CTGCTGTGCT TCAGTCAGCT GGGAGTGTAC 
GTGGACGCGA CTGGAAGACG ATCACGAACT CAGGAGCTCA TGTGGCCCTC CACACCGCTC 
GCCTGCAGCT CAAACTCGAA CTGCCTGACG GTGTACAGCG ATTATGGGGT CGACGTGTTT 
GATGTTCACA CTATGGAATG GGTTCAGACC ATCTCATTAA GAAAAATTCG ACCTCTGAAT 
GTGGAAGGCA GTCTAAACCT TTTGAGCATT GAGCAACCCC ATCTCATCTA TTTCAGCAAC 
AACTCCTCGG ATGGCTGTGA CCTGGCCATC CCCGAGACCT CAGACAACAG CAAGAAGCTG 
ATGGTGAGGA CGCGCAGCAA GAGGAAGTTC CTTTTTAAAG TACCAGAGGA GGAGCGGCTG 
CAGCAGAGAA GGGAGATGCT GAGGGATCCT GAAATGAGAT CCAAGCTAAT CTCTCACCCC 
ACCAACTTCA ATCACGTTGC TCACATGGGC CCAGGCGACG GCATGCAGGT CCTCATGGAT 
CTGCCTCTGA GTGTGATGCC CTCTTCTCAG GATGACCTGT CTAAAGACAA ACCTCGACCT 
CTGTCTAGCA TCTCACGCCA ACAGAGGAGC AAGACCCACA TCACCCGTAC CGCCTCAGGA 
GGGGGAGACT TCGGAGGAGC AGGGTCGTCC CGCAGTATTT CTGATCAGGA TCAAGACTAC 
GAGCGAGAGC CCGACTCAGA CTCGACCAAG CATTCCACCC CCTCGAACAG CTCCAACCCC 
AGCAGCCCAC CGAGCCCCAA CTCTCCCCAC CGCAGTCAGC TGACTCTGGA CAGCCTGGAT 
CAGTCCCTGG ATGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPB ( XM_009294622.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009292897.1 Danio rerio CDC42 binding protein kinase beta (DMPK-like) (cdc42bpb), transcript variant X1, mRNA.

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CloneID	ODa45054
Clone ID Related Accession (Same CDS sequence)	XM_009294622.3 , XM_009294622.2
Accession Version	XM_009294622.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5178bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase MRCK beta isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294622.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCGA AGGTGCGCCT GAAGAAGTTG GAGCAGTTGC TGCTGGATGG CCATCAGAAA 
AACGACAAGT CACTGAGTGT GGAGACACTG CTGGATATTC TCATATGTTT ATACAACGAG 
TGCAGCAACT CGCCCTTAAA ACGGGAAAAA CACGTGACGG ACTTTTTGGA ATGGGTGAAG 
CCGTTTACAA CCACTGTGAA AGAGATGCGC CTTCACAGAG ACGACTTCGA GATGCTGAAA 
GTGATCGGCA GGGGAGCCTT TGGAGAGGTT GCTGTGGTAA AGATGAAGCA CACAGAGCGG 
GTTTACGCCA TGAAGATCCT CAACAAGTGG GAGATGCTGA AAAGGGCTGA GACGGCATGC 
TTTCGGGAAG AGAGGAACGT CCTGGTGAAT GGAGACTGCC AGTGGATCAC TACCCTTCAT 
TATGCTTTTC AGGATGACAA CTATCTGTAC TTGGTGATGG ATTATTACGT TGGCGGTGAC 
CTGCTGACGC TGCTTAGTAA ATTTGAAGAC CGGCTGCCGG AGGACATGGC CAAGTTCTAC 
GTGGCGGAGA TGGTTCTCGC CATCCACTCC ATCCATCAGC AGCGCTACGT ACACAGGGAT 
ATAAAACCCG ACAACGTGCT GCTGGACATG AACGGCCACA TCCGACTCGC TGACTTCGGC 
TCCTGTCTGA AGATGATGCA AGACGGCACG GTCCAATCTT CAGTGGCTGT GGGCACACCA 
GACTACATCT CTCCAGAGAT CCTGCAGGCC ATGGAGGACG GCATGGGGAA ATACGGGCCG 
GAGTGTGACT GGTGGTCGCT GGGCGTTTGC ATGTATGAGA TGCTGTACGG CGAGACACCC 
TTTTACGCCG AATCCCTGGT GGAGACCTAT GGAAAGATCA TGAACCATGA GGAGCGTTTC 
CAGTTCCCTT CCCACATCAC GGATGTATCC GAGGATGCTA AAGACCTGAT CCAGAGGCTG 
ATTTGCAGCC GAGAGCGCCG TTTGGGTCAA CATGGCATCG AGGAGTTCAG GAAGCACGCC 
TTTTTCTCCG GCATCGACTG GGAGAACATC CGGAACATGG AGGCGCCATA CATCCCTGAC 
GTCAGCTCTC CATCAGACAC CTCCAACTTT GATGTAGATG ATGACGTACT GAAGAATCCA 
GACATTGCTC CTCCAGTTTC TCACACGGGT TTCACCGGGC AGCACTTGCC CTTTGTGGGC 
TTCACCTACA CTACAGAGAG CTGTTTCTCA GACGGCGGCA GCATGAGGGG TGTGGCGCTG 
TCTGACGGGG GCACCGGGGA GGATCTCCAG GATGGAGGCA TGCAGGTGGA GGCCTTTGAG 
AAGAGGATTC GCTGCCTGGA GCAAGAGAAA TTGGAGCTTA ACCGTAAGCT GCAAGAATCC 
ACTCAGGCTG TTCAATCTCT TCATGGCTCT GGTCGAGGAG CCGGCACTCT CGGACGAGAT 
AAAGAGATTA AAAAACTTAA TGAGGAAATC GATCGGCTTA AAAAGAAACT GGCAGACTCT 
GATAGGCTGG AGCACCAGTT GGAGGAAGCA GTGACTCTTC GACAGGACTT TGAAAGTTCC 
TCCACCAAAC TAAAAGCCCT GGACAAGCAA GTCAAAGCTC TCAAGCTGGA GAAAGAGGAT 
ATTCATAAGC AACTGGTGGA GTCTTTGGAC CGCCTGAAGT CTCAGACCAA GGAGCTGAAG 
GACGCGCACC AGCAGAGGAA GCTGGCCATG CAGGAGTTCT CGGAGGTGAA CGAGCGTATG 
GCCGAACTGC GCTCCCAGAA GCAGCGTCTG TCGCGCCAGC TGCGGGACAA GGAGGAGGAG 
ATGGAGGTGG TGATGCAGAA AATCGACGCC ATGCGCCAGG ACATCCGCAA AACCGAGAAG 
GCCCGCAAGG AGCTGGAGTC TCAGCTGGAG GATGCGCGTG CCGAAGCTTC TAAGGAGCGT 
AAACTTCGAG AGCACAGTGA GGTTTACTCC AAACAACTGG AGAGTGAACT GGAGACACTC 
AAGGTGAAGC AGGGCGCAGG TCGTGCGTCA GGCCTCGGTG CAGAGACTCA GCAGGAGCTC 
ACCAAACTCA AGTCTGAGCT AGATAAGAAA GGACTGTATT ATGAGGAAGA GCTGGTGAGA 
CGTGAAGCCA GTCACACTAC AGAGATGAAA AACCTGAGGA AGGAGCTCCA GGACTCTGAG 
GGTCAGCAGC TCTCCCTGCA CAAAGAGCTG CTTGCGCTTA AAGAAAAACT GGAAAAGGCC 
AAGAGAGAAC GGCAAATTGA GATGGAAGAG GCCATTAATG CCCTGAAAGA GCAGTATGAA 
CGTGAACGCA ACCTTATGAG TGAAGACAAC CGCAAACTCA CTGCAGAAAC AGACCGGCTC 
TGCAGTTTTG TGGACAAGCT GACCGCTCAG AACAGGCAAC TGGAGGACGA GCTCCAGGAA 
CTGGCATCAA AGAAGGAGAG CGTAGCACAC TGGGAAGCAC AGATCGCAGA GATTATCCAG 
TGGGTGAGCG ATGAGAAAGA CGCACGCGGC TACCTTCAGG CTCTGGCCTC CAAGATGACA 
GAAGAGTTGG AGTCTCTGCG CAGCTCCAGT TTGGGATCCA GAACACTGGA TCCGTTGTGG 
AAGGTGCGTC GCAGTCAAAA GCTGGATATG TCTGCCCGTC TAGAGCTGCA GTCGGCCTTG 
GAAGCTGAAA TCCGTGCCAA GCAACTGGTT CACGATGAAC TGCGCAAGGT CAAAGCTGCC 
AACATCTCTT TCGAGAGCAA ACTGAAGGAC ACCGAGGCCA AGAACAGAGA GTTGGAGGAA 
CAGCTGGAGA ACATGAAGAA GGAGATGGAG GAGAGCCGCT CACGATCAGA CAAAGGCCTG 
AAACTGCCAG ACTTCCAGGA CTCCATCTTT GACTACTTCA ATACCTCTCC TCTTGCCCAT 
GAGCTGACAT TCAGAGACTC TGTCTCCTCC TCATCTGCAT CTTCTCTGCT TGCATTTTGG 
GACGAAACCA GCTCTGTCGG AGATGTGGAC GTCGCACCTC AGAAGACTGA CATTCCCCCT 
TCCTCCCCAT CAGCAGCTAC AGAACAGGAT GAAGCAGTCA AGCCTCAAGC GACCACTCCT 
GCTACTCCTT CTCCAATGTA TCAGCCCACT CTCACTGCAC CAAAGCCCAA AGCCCATCAG 
CTGAGCATCA AGACCTTCTC CAGCCCGACT CAGTGCACTC ACTGTACTTC ACTGATGGTG 
GGCCAGATGC GTCAAGGATA CGCCTGCGAG GTATGCTCTT TCATCTGCCA CGTGTCCTGT 
AAAGACAACG CACCCCAGCT CTGCCCCATT CCTCCCGAGC AGACCAAGAG GCCTCTGGGT 
ATTGATGTGC AGAGGGGCAT TGGCACTGCG TACAAAGGTT TTGTCAGGAT CCCGAAGCCC 
ACCGGAGTGA AGAAGGGCTG GCAGAGAGCA TATGCTGTAG TGTGTGACTG TAAACTCTTT 
CTCTACGAGG TGCCAGAAGG GAAGTCCACT CAACCTGTCG TGATGGCCAG TCAGGTCCTT 
GACTTGAGGG ATGAGGAGTT CTCTGTTAGC TCTGTGTTGG CATCTGATGT GATCCATGCC 
AACCATAAAG ACGTTCCCTG TATATTCAGG GTAACCTCGT CGGCTCTGAA ATCCTCGATC 
CGGCCAGTTC CTTTGCTGAT TTTAGCTGAG AGCGAGGCGG AGAAGAGGAA GTGGGTGGGC 
ATCCTAGAGG GCCTGCAGAG CATCCTAGCC AAAAACCGGC TGAAGAACCG CGTGGTGCAT 
GTGCTGCACG AGGCCTACGA CAGCAGCCTA CCTGCCATCA AAACTACACT TTCTGCTGCC 
ATCGTAGATC GAGAACGAAT TGTCTTGGGG ACAGAAGATG GCCTTTTTGT AGTCGAGATC 
ACCAGAGATG TCATTGTTCG TGCCGCTGAC TGTAAAAAAG TCTGCCAGAT CGAGCTCATC 
CCCAAAGAGA AGATGGTGGC CCTGCTTTCT GGCCGTAATC GCCATGTCCA TTTATACCCC 
TGGGCGGCAC TAGAGGGCGC TGAAGGGGGT TTCGATGCCA AGCTGACAGA TACGAGAGGC 
TGCCAGGCCA TGACCATCGG TAACCTCCGC CCTGGCGGTC CAGCGTGTCT CTTTGCAGGC 
GTGAAGCGGC AGGTTTTCTG TTATGAAATC ACACGAGCGA AACCCCATCA TCGCAAACTC 
TGGGAAGTGC AGGCGCCAGG GGTCGTCCAA TGGCTGGGGA TAATTCGAGA ACGGATCTGC 
GTAGGTTACC CATCAGGCTT TGCTCTGCTG GCCATGCAGG GAGAATCGTC GCCGGTCAGT 
CTGGTGAGCC CAGGTGACCC CTCGCTGGCA TTTTTGGCCC AGCAGCCTCT GGATGCACTT 
CATGCTATGG AAGTGGGGTC CAATGAGCTG CTGCTGTGCT TCAGTCAGCT GGGAGTGTAC 
GTGGACGCGA CTGGAAGACG ATCACGAACT CAGGAGCTCA TGTGGCCCTC CACACCGCTC 
GCCTGCAGCT CAAACTCGAA CTGCCTGACG GTGTACAGCG ATTATGGGGT CGACGTGTTT 
GATGTTCACA CTATGGAATG GGTTCAGACC ATCTCATTAA GAAAAATTCG ACCTCTGAAT 
GTGGAAGGCA GTCTAAACCT TTTGAGCATT GAGCAACCCC ATCTCATCTA TTTCAGCAAC 
AACTCCTCGG ATGGCTGTGA CCTGGCCATC CCCGAGACCT CAGACAACAG CAAGAAGCTG 
ATGGTGAGGA CGCGCAGCAA GAGGAAGTTC CTTTTTAAAG TACCAGAGGA GGAGCGGCTG 
CAGCAGAGAA GGGAGATGCT GAGGGATCCT GAAATGAGAT CCAAGCTAAT CTCTCACCCC 
ACCAACTTCA ATCACGTTGC TCACATGGGC CCAGGCGACG GCATGCAGGT CCTCATGGAT 
CTGCCTCTGA GTGTGATGCC CTCTTCTCAG GATGACCTGT CTAAAGACAA ACCTCGACCT 
CTGTCTAGCA TCTCACGCCA ACAGAGGAGC AAGACCCACA TCACCCGTAC CGCCTCAGGA 
GGGGGAGACT TCGGAGGAGC AGGGTCGTCC CGCAGTATTT CTGATCAGGA TCAAGACTAC 
GAGCGAGAGC CCGACTCAGA CTCGACCAAG CATTCCACCC CCTCGAACAG CTCCAACCCC 
AGCAGCCCAC CGAGCCCCAA CTCTCCCCAC CGCAGTCAGC TGACTCTGGA CAGCCTGGAT 
CAGTCCCTGG ATGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292897.1
CDS	853..6030
Translation	MSAKVRLKKLEQLLLDGHQKNDKSLSVETLLDILICLYNECSNSPLKREKHVTDFLEWVKPFTTTVKEMRLHRDDFEMLKVIGRGAFGEVAVVKMKHTERVYAMKILNKWEMLKRAETACFREERNVLVNGDCQWITTLHYAFQDDNYLYLVMDYYVGGDLLTLLSKFEDRLPEDMAKFYVAEMVLAIHSIHQQRYVHRDIKPDNVLLDMNGHIRLADFGSCLKMMQDGTVQSSVAVGTPDYISPEILQAMEDGMGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHEERFQFPSHITDVSEDAKDLIQRLICSRERRLGQHGIEEFRKHAFFSGIDWENIRNMEAPYIPDVSSPSDTSNFDVDDDVLKNPDIAPPVSHTGFTGQHLPFVGFTYTTESCFSDGGSMRGVALSDGGTGEDLQDGGMQVEAFEKRIRCLEQEKLELNRKLQESTQAVQSLHGSGRGAGTLGRDKEIKKLNEEIDRLKKKLADSDRLEHQLEEAVTLRQDFESSSTKLKALDKQVKALKLEKEDIHKQLVESLDRLKSQTKELKDAHQQRKLAMQEFSEVNERMAELRSQKQRLSRQLRDKEEEMEVVMQKIDAMRQDIRKTEKARKELESQLEDARAEASKERKLREHSEVYSKQLESELETLKVKQGAGRASGLGAETQQELTKLKSELDKKGLYYEEELVRREASHTTEMKNLRKELQDSEGQQLSLHKELLALKEKLEKAKRERQIEMEEAINALKEQYERERNLMSEDNRKLTAETDRLCSFVDKLTAQNRQLEDELQELASKKESVAHWEAQIAEIIQWVSDEKDARGYLQALASKMTEELESLRSSSLGSRTLDPLWKVRRSQKLDMSARLELQSALEAEIRAKQLVHDELRKVKAANISFESKLKDTEAKNRELEEQLENMKKEMEESRSRSDKGLKLPDFQDSIFDYFNTSPLAHELTFRDSVSSSSASSLLAFWDETSSVGDVDVAPQKTDIPPSSPSAATEQDEAVKPQATTPATPSPMYQPTLTAPKPKAHQLSIKTFSSPTQCTHCTSLMVGQMRQGYACEVCSFICHVSCKDNAPQLCPIPPEQTKRPLGIDVQRGIGTAYKGFVRIPKPTGVKKGWQRAYAVVCDCKLFLYEVPEGKSTQPVVMASQVLDLRDEEFSVSSVLASDVIHANHKDVPCIFRVTSSALKSSIRPVPLLILAESEAEKRKWVGILEGLQSILAKNRLKNRVVHVLHEAYDSSLPAIKTTLSAAIVDRERIVLGTEDGLFVVEITRDVIVRAADCKKVCQIELIPKEKMVALLSGRNRHVHLYPWAALEGAEGGFDAKLTDTRGCQAMTIGNLRPGGPACLFAGVKRQVFCYEITRAKPHHRKLWEVQAPGVVQWLGIIRERICVGYPSGFALLAMQGESSPVSLVSPGDPSLAFLAQQPLDALHAMEVGSNELLLCFSQLGVYVDATGRRSRTQELMWPSTPLACSSNSNCLTVYSDYGVDVFDVHTMEWVQTISLRKIRPLNVEGSLNLLSIEQPHLIYFSNNSSDGCDLAIPETSDNSKKLMVRTRSKRKFLFKVPEEERLQQRREMLRDPEMRSKLISHPTNFNHVAHMGPGDGMQVLMDLPLSVMPSSQDDLSKDKPRPLSSISRQQRSKTHITRTASGGGDFGGAGSSRSISDQDQDYEREPDSDSTKHSTPSNSSNPSSPPSPNSPHRSQLTLDSLDQSLDG

Target ORF information:

RefSeq Version	XM_009294622.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio CDC42 binding protein kinase beta (DMPK-like) (cdc42bpb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294622.2

ORF Insert Sequence:

ATGTCTGCGA AGGTGCGCCT GAAGAAGTTG GAGCAGTTGC TGCTGGATGG CCATCAGAAA 
AACGACAAGT CACTGAGTGT GGAGACACTG CTGGATATTC TCATATGTTT ATACAACGAG 
TGCAGCAACT CGCCCTTAAA ACGGGAAAAA CACGTGACGG ACTTTTTGGA ATGGGTGAAG 
CCGTTTACAA CCACTGTGAA AGAGATGCGC CTTCACAGAG ACGACTTCGA GATGCTGAAA 
GTGATCGGCA GGGGAGCCTT TGGAGAGGTT GCTGTGGTAA AGATGAAGCA CACAGAGCGG 
GTTTACGCCA TGAAGATCCT CAACAAGTGG GAGATGCTGA AAAGGGCTGA GACGGCATGC 
TTTCGGGAAG AGAGGAACGT CCTGGTGAAT GGAGACTGCC AGTGGATCAC TACCCTTCAT 
TATGCTTTTC AGGATGACAA CTATCTGTAC TTGGTGATGG ATTATTACGT TGGCGGTGAC 
CTGCTGACGC TGCTTAGTAA ATTTGAAGAC CGGCTGCCGG AGGACATGGC CAAGTTCTAC 
GTGGCGGAGA TGGTTCTCGC CATCCACTCC ATCCATCAGC AGCGCTACGT ACACAGGGAT 
ATAAAACCCG ACAACGTGCT GCTGGACATG AACGGCCACA TCCGACTCGC TGACTTCGGC 
TCCTGTCTGA AGATGATGCA AGACGGCACG GTCCAATCTT CAGTGGCTGT GGGCACACCA 
GACTACATCT CTCCAGAGAT CCTGCAGGCC ATGGAGGACG GCATGGGGAA ATACGGGCCG 
GAGTGTGACT GGTGGTCGCT GGGCGTTTGC ATGTATGAGA TGCTGTACGG CGAGACACCC 
TTTTACGCCG AATCCCTGGT GGAGACCTAT GGAAAGATCA TGAACCATGA GGAGCGTTTC 
CAGTTCCCTT CCCACATCAC GGATGTATCC GAGGATGCTA AAGACCTGAT CCAGAGGCTG 
ATTTGCAGCC GAGAGCGCCG TTTGGGTCAA CATGGCATCG AGGAGTTCAG GAAGCACGCC 
TTTTTCTCCG GCATCGACTG GGAGAACATC CGGAACATGG AGGCGCCATA CATCCCTGAC 
GTCAGCTCTC CATCAGACAC CTCCAACTTT GATGTAGATG ATGACGTACT GAAGAATCCA 
GACATTGCTC CTCCAGTTTC TCACACGGGT TTCACCGGGC AGCACTTGCC CTTTGTGGGC 
TTCACCTACA CTACAGAGAG CTGTTTCTCA GACGGCGGCA GCATGAGGGG TGTGGCGCTG 
TCTGACGGGG GCACCGGGGA GGATCTCCAG GATGGAGGCA TGCAGGTGGA GGCCTTTGAG 
AAGAGGATTC GCTGCCTGGA GCAAGAGAAA TTGGAGCTTA ACCGTAAGCT GCAAGAATCC 
ACTCAGGCTG TTCAATCTCT TCATGGCTCT GGTCGAGGAG CCGGCACTCT CGGACGAGAT 
AAAGAGATTA AAAAACTTAA TGAGGAAATC GATCGGCTTA AAAAGAAACT GGCAGACTCT 
GATAGGCTGG AGCACCAGTT GGAGGAAGCA GTGACTCTTC GACAGGACTT TGAAAGTTCC 
TCCACCAAAC TAAAAGCCCT GGACAAGCAA GTCAAAGCTC TCAAGCTGGA GAAAGAGGAT 
ATTCATAAGC AACTGGTGGA GTCTTTGGAC CGCCTGAAGT CTCAGACCAA GGAGCTGAAG 
GACGCGCACC AGCAGAGGAA GCTGGCCATG CAGGAGTTCT CGGAGGTGAA CGAGCGTATG 
GCCGAACTGC GCTCCCAGAA GCAGCGTCTG TCGCGCCAGC TGCGGGACAA GGAGGAGGAG 
ATGGAGGTGG TGATGCAGAA AATCGACGCC ATGCGCCAGG ACATCCGCAA AACCGAGAAG 
GCCCGCAAGG AGCTGGAGTC TCAGCTGGAG GATGCGCGTG CCGAAGCTTC TAAGGAGCGT 
AAACTTCGAG AGCACAGTGA GGTTTACTCC AAACAACTGG AGAGTGAACT GGAGACACTC 
AAGGTGAAGC AGGGCGCAGG TCGTGCGTCA GGCCTCGGTG CAGAGACTCA GCAGGAGCTC 
ACCAAACTCA AGTCTGAGCT AGATAAGAAA GGACTGTATT ATGAGGAAGA GCTGGTGAGA 
CGTGAAGCCA GTCACACTAC AGAGATGAAA AACCTGAGGA AGGAGCTCCA GGACTCTGAG 
GGTCAGCAGC TCTCCCTGCA CAAAGAGCTG CTTGCGCTTA AAGAAAAACT GGAAAAGGCC 
AAGAGAGAAC GGCAAATTGA GATGGAAGAG GCCATTAATG CCCTGAAAGA GCAGTATGAA 
CGTGAACGCA ACCTTATGAG TGAAGACAAC CGCAAACTCA CTGCAGAAAC AGACCGGCTC 
TGCAGTTTTG TGGACAAGCT GACCGCTCAG AACAGGCAAC TGGAGGACGA GCTCCAGGAA 
CTGGCATCAA AGAAGGAGAG CGTAGCACAC TGGGAAGCAC AGATCGCAGA GATTATCCAG 
TGGGTGAGCG ATGAGAAAGA CGCACGCGGC TACCTTCAGG CTCTGGCCTC CAAGATGACA 
GAAGAGTTGG AGTCTCTGCG CAGCTCCAGT TTGGGATCCA GAACACTGGA TCCGTTGTGG 
AAGGTGCGTC GCAGTCAAAA GCTGGATATG TCTGCCCGTC TAGAGCTGCA GTCGGCCTTG 
GAAGCTGAAA TCCGTGCCAA GCAACTGGTT CACGATGAAC TGCGCAAGGT CAAAGCTGCC 
AACATCTCTT TCGAGAGCAA ACTGAAGGAC ACCGAGGCCA AGAACAGAGA GTTGGAGGAA 
CAGCTGGAGA ACATGAAGAA GGAGATGGAG GAGAGCCGCT CACGATCAGA CAAAGGCCTG 
AAACTGCCAG ACTTCCAGGA CTCCATCTTT GACTACTTCA ATACCTCTCC TCTTGCCCAT 
GAGCTGACAT TCAGAGACTC TGTCTCCTCC TCATCTGCAT CTTCTCTGCT TGCATTTTGG 
GACGAAACCA GCTCTGTCGG AGATGTGGAC GTCGCACCTC AGAAGACTGA CATTCCCCCT 
TCCTCCCCAT CAGCAGCTAC AGAACAGGAT GAAGCAGTCA AGCCTCAAGC GACCACTCCT 
GCTACTCCTT CTCCAATGTA TCAGCCCACT CTCACTGCAC CAAAGCCCAA AGCCCATCAG 
CTGAGCATCA AGACCTTCTC CAGCCCGACT CAGTGCACTC ACTGTACTTC ACTGATGGTG 
GGCCAGATGC GTCAAGGATA CGCCTGCGAG GTATGCTCTT TCATCTGCCA CGTGTCCTGT 
AAAGACAACG CACCCCAGCT CTGCCCCATT CCTCCCGAGC AGACCAAGAG GCCTCTGGGT 
ATTGATGTGC AGAGGGGCAT TGGCACTGCG TACAAAGGTT TTGTCAGGAT CCCGAAGCCC 
ACCGGAGTGA AGAAGGGCTG GCAGAGAGCA TATGCTGTAG TGTGTGACTG TAAACTCTTT 
CTCTACGAGG TGCCAGAAGG GAAGTCCACT CAACCTGTCG TGATGGCCAG TCAGGTCCTT 
GACTTGAGGG ATGAGGAGTT CTCTGTTAGC TCTGTGTTGG CATCTGATGT GATCCATGCC 
AACCATAAAG ACGTTCCCTG TATATTCAGG GTAACCTCGT CGGCTCTGAA ATCCTCGATC 
CGGCCAGTTC CTTTGCTGAT TTTAGCTGAG AGCGAGGCGG AGAAGAGGAA GTGGGTGGGC 
ATCCTAGAGG GCCTGCAGAG CATCCTAGCC AAAAACCGGC TGAAGAACCG CGTGGTGCAT 
GTGCTGCACG AGGCCTACGA CAGCAGCCTA CCTGCCATCA AAACTACACT TTCTGCTGCC 
ATCGTAGATC GAGAACGAAT TGTCTTGGGG ACAGAAGATG GCCTTTTTGT AGTCGAGATC 
ACCAGAGATG TCATTGTTCG TGCCGCTGAC TGTAAAAAAG TCTGCCAGAT CGAGCTCATC 
CCCAAAGAGA AGATGGTGGC CCTGCTTTCT GGCCGTAATC GCCATGTCCA TTTATACCCC 
TGGGCGGCAC TAGAGGGCGC TGAAGGGGGT TTCGATGCCA AGCTGACAGA TACGAGAGGC 
TGCCAGGCCA TGACCATCGG TAACCTCCGC CCTGGCGGTC CAGCGTGTCT CTTTGCAGGC 
GTGAAGCGGC AGGTTTTCTG TTATGAAATC ACACGAGCGA AACCCCATCA TCGCAAACTC 
TGGGAAGTGC AGGCGCCAGG GGTCGTCCAA TGGCTGGGGA TAATTCGAGA ACGGATCTGC 
GTAGGTTACC CATCAGGCTT TGCTCTGCTG GCCATGCAGG GAGAATCGTC GCCGGTCAGT 
CTGGTGAGCC CAGGTGACCC CTCGCTGGCA TTTTTGGCCC AGCAGCCTCT GGATGCACTT 
CATGCTATGG AAGTGGGGTC CAATGAGCTG CTGCTGTGCT TCAGTCAGCT GGGAGTGTAC 
GTGGACGCGA CTGGAAGACG ATCACGAACT CAGGAGCTCA TGTGGCCCTC CACACCGCTC 
GCCTGCAGCT CAAACTCGAA CTGCCTGACG GTGTACAGCG ATTATGGGGT CGACGTGTTT 
GATGTTCACA CTATGGAATG GGTTCAGACC ATCTCATTAA GAAAAATTCG ACCTCTGAAT 
GTGGAAGGCA GTCTAAACCT TTTGAGCATT GAGCAACCCC ATCTCATCTA TTTCAGCAAC 
AACTCCTCGG ATGGCTGTGA CCTGGCCATC CCCGAGACCT CAGACAACAG CAAGAAGCTG 
ATGGTGAGGA CGCGCAGCAA GAGGAAGTTC CTTTTTAAAG TACCAGAGGA GGAGCGGCTG 
CAGCAGAGAA GGGAGATGCT GAGGGATCCT GAAATGAGAT CCAAGCTAAT CTCTCACCCC 
ACCAACTTCA ATCACGTTGC TCACATGGGC CCAGGCGACG GCATGCAGGT CCTCATGGAT 
CTGCCTCTGA GTGTGATGCC CTCTTCTCAG GATGACCTGT CTAAAGACAA ACCTCGACCT 
CTGTCTAGCA TCTCACGCCA ACAGAGGAGC AAGACCCACA TCACCCGTAC CGCCTCAGGA 
GGGGGAGACT TCGGAGGAGC AGGGTCGTCC CGCAGTATTT CTGATCAGGA TCAAGACTAC 
GAGCGAGAGC CCGACTCAGA CTCGACCAAG CATTCCACCC CCTCGAACAG CTCCAACCCC 
AGCAGCCCAC CGAGCCCCAA CTCTCCCCAC CGCAGTCAGC TGACTCTGGA CAGCCTGGAT 
CAGTCCCTGG ATGGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CDC42BPB ( XM_009294623.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009292898.1 Danio rerio CDC42 binding protein kinase beta (DMPK-like) (cdc42bpb), transcript variant X2, mRNA.

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CloneID	ODa45055
Clone ID Related Accession (Same CDS sequence)	XM_009294623.2
Accession Version	XM_009294623.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5070bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase MRCK beta isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294623.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGCGA AGGTGCGCCT GAAGAAGTTG GAGCAGTTGC TGCTGGATGG CCATCAGAAA 
AACGACAAGT CACTGAGTGT GGAGACACTG CTGGATATTC TCATATGTTT ATACAACGAG 
TGCAGCAACT CGCCCTTAAA ACGGGAAAAA CACGTGACGG ACTTTTTGGA ATGGGTGAAG 
CCGTTTACAA CCACTGTGAA AGAGATGCGC CTTCACAGAG ACGACTTCGA GATGCTGAAA 
GTGATCGGCA GGGGAGCCTT TGGAGAGGTT GCTGTGGTAA AGATGAAGCA CACAGAGCGG 
GTTTACGCCA TGAAGATCCT CAACAAGTGG GAGATGCTGA AAAGGGCTGA GACGGCATGC 
TTTCGGGAAG AGAGGAACGT CCTGGTGAAT GGAGACTGCC AGTGGATCAC TACCCTTCAT 
TATGCTTTTC AGGATGACAA CTATCTGTAC TTGGTGATGG ATTATTACGT TGGCGGTGAC 
CTGCTGACGC TGCTTAGTAA ATTTGAAGAC CGGCTGCCGG AGGACATGGC CAAGTTCTAC 
GTGGCGGAGA TGGTTCTCGC CATCCACTCC ATCCATCAGC AGCGCTACGT ACACAGGGAT 
ATAAAACCCG ACAACGTGCT GCTGGACATG AACGGCCACA TCCGACTCGC TGACTTCGGC 
TCCTGTCTGA AGATGATGCA AGACGGCACG GTCCAATCTT CAGTGGCTGT GGGCACACCA 
GACTACATCT CTCCAGAGAT CCTGCAGGCC ATGGAGGACG GCATGGGGAA ATACGGGCCG 
GAGTGTGACT GGTGGTCGCT GGGCGTTTGC ATGTATGAGA TGCTGTACGG CGAGACACCC 
TTTTACGCCG AATCCCTGGT GGAGACCTAT GGAAAGATCA TGAACCATGA GGAGCGTTTC 
CAGTTCCCTT CCCACATCAC GGATGTATCC GAGGATGCTA AAGACCTGAT CCAGAGGCTG 
ATTTGCAGCC GAGAGCGCCG TTTGGGTCAA CATGGCATCG AGGAGTTCAG GAAGCACGCC 
TTTTTCTCCG GCATCGACTG GGAGAACATC CGGAACATGG AGGCGCCATA CATCCCTGAC 
GTCAGCTCTC CATCAGACAC CTCCAACTTT GATGTAGATG ATGACGTACT GAAGAATCCA 
GACATTGCTC CTCCAGTTTC TCACACGGGT TTCACCGGGC AGCACTTGCC CTTTGTGGGC 
TTCACCTACA CTACAGAGAG CTGTTTCTCA GACGGCGGCA GCATGAGGGG TGTGGCGCTG 
TCTGACGGGG GCACCGGGGA GGATCTCCAG GATGGAGGCA TGCAGGTGGA GGCCTTTGAG 
AAGAGGATTC GCTGCCTGGA GCAAGAGAAA TTGGAGCTTA ACCGTAAGCT GCAAGAATCC 
ACTCAGGCTG TTCAATCTCT TCATGGCTCT GGTCGAGGAG CCGGCACTCT CGGACGAGAT 
AAAGAGATTA AAAAACTTAA TGAGGAAATC GATCGGCTTA AAAAGAAACT GGCAGACTCT 
GATAGGCTGG AGCACCAGTT GGAGGAAGCA GTGACTCTTC GACAGGACTT TGAAAGTTCC 
TCCACCAAAC TAAAAGCCCT GGACAAGCAA GTCAAAGCTC TCAAGCTGGA GAAAGAGGAT 
ATTCATAAGC AACTGGTGGA GTCTTTGGAC CGCCTGAAGT CTCAGACCAA GGAGCTGAAG 
GACGCGCACC AGCAGAGGAA GCTGGCCATG CAGGAGTTCT CGGAGGTGAA CGAGCGTATG 
GCCGAACTGC GCTCCCAGAA GCAGCGTCTG TCGCGCCAGC TGCGGGACAA GGAGGAGGAG 
ATGGAGGTGG TGATGCAGAA AATCGACGCC ATGCGCCAGG ACATCCGCAA AACCGAGAAG 
GCCCGCAAGG AGCTGGAGTC TCAGCTGGAG GATGCGCGTG CCGAAGCTTC TAAGGAGCGT 
AAACTTCGAG AGCACAGTGA GGTTTACTCC AAACAACTGG AGAGTGAACT GGAGACACTC 
AAGGTGAAGC AGGGCGCAGG TCGTGCGTCA GGCCTCGGTG CAGAGACTCA GCAGGAGCTC 
ACCAAACTCA AGTCTGAGCT AGATAAGAAA GGACTGTATT ATGAGGAAGA GCTGGTGAGA 
CGTGAAGCCA GTCACACTAC AGAGATGAAA AACCTGAGGA AGGAGCTCCA GGACTCTGAG 
GGTCAGCAGC TCTCCCTGCA CAAAGAGCTG CTTGCGCTTA AAGAAAAACT GGAAAAGGCC 
AAGAGAGAAC GGCAAATTGA GATGGAAGAG GCCATTAATG CCCTGAAAGA GCAGTATGAA 
CGTGAACGCA ACCTTATGAG TGAAGACAAC CGCAAACTCA CTGCAGAAAC AGACCGGCTC 
TGCAGTTTTG TGGACAAGCT GACCGCTCAG AACAGGCAAC TGGAGGACGA GCTCCAGGAA 
CTGGCATCAA AGAAGGAGAG CGTAGCACAC TGGGAAGCAC AGATCGCAGA GATTATCCAG 
TGGGTGAGCG ATGAGAAAGA CGCACGCGGC TACCTTCAGG CTCTGGCCTC CAAGATGACA 
GAAGAGTTGG AGTCTCTGCG CAGCTCCAGT TTGGGATCCA GAACACTGGA TCCGTTGTGG 
AAGGTGCGTC GCAGTCAAAA GCTGGATATG TCTGCCCGTC TAGAGCTGCA GTCGGCCTTG 
GAAGCTGAAA TCCGTGCCAA GCAACTGGTT CACGATGAAC TGCGCAAGGT CAAAGCTGCC 
AACATCTCTT TCGAGAGCAA ACTGAAGGAC ACCGAGGCCA AGAACAGAGA GTTGGAGGAA 
CAGCTGGAGA ACATGAAGAA GGAGATGGAG GAGAGCCGCT CACGATCAGA CAAAGGCCTG 
AAACTGCCAG ACTTCCAGGA CTCCATCTTT GACTACTTCA ATACCTCTCC TCTTGCCCAT 
GAGCTGACAT TCAGAGACTC TGTCTCCTCC TCATCTGCAT CTTCTCTGCT TGCATTTTGG 
GACGAAACCA GCTCTGTCGG AGATGTGGAC GTCGCACCTC AGAAGACTGA CATTCCCCCT 
TCCTCCCCAT CAGCAGCTAC AGAACAGGAT GAAGCAGTCA AGCCTCAAGC GACCACTCCT 
GCTACTCCTT CTCCAATGTA TCAGCCCACT CTCACTGCAC CAAAGCCCAA AGCCCATCAG 
CTGAGCATCA AGACCTTCTC CAGCCCGACT CAGTGCACTC ACTGTACTTC ACTGATGGTG 
GGCCAGATGC GTCAAGGATA CGCCTGCGAG GTATGCTCTT TCATCTGCCA CGTGTCCTGT 
AAAGACAACG CACCCCAGCT CTGCCCCATT CCTCCCGAGC AGACCAAGAG GCCTCTGGGT 
ATTGATGTGC AGAGGGGCAT TGGCACTGCG TACAAAGGTT TTGTCAGGAT CCCGAAGCCC 
ACCGGAGTGA AGAAGGGCTG GCAGAGAGCA TATGCTGTAG TGTGTGACTG TAAACTCTTT 
CTCTACGAGG TGCCAGAAGG GAAGTCCACT CAACCTGTCG TGATGGCCAG TCAGGTCCTT 
GACTTGAGGG ATGAGGAGTT CTCTGTTAGC TCTGTGTTGG CATCTGATGT GATCCATGCC 
AACCATAAAG ACGTTCCCTG TATATTCAGG GTAACCTCGT CGGCTCTGAA ATCCTCGATC 
CGGCCAGTTC CTTTGCTGAT TTTAGCTGAG AGCGAGGCGG AGAAGAGGAA GTGGGTGGGC 
ATCCTAGAGG GCCTGCAGAG CATCCTAGCC AAAAACCGGC TGAAGAACCG CGTGGTGCAT 
GTGCTGCACG AGGCCTACGA CAGCAGCCTA CCTGCCATCA AAACTACACT TTCTGCTGCC 
ATCGTAGATC GAGAACGAAT TGTCTTGGGG ACAGAAGATG GCCTTTTTGT AGTCGAGATC 
ACCAGAGATG TCATTGTTCG TGCCGCTGAC TGTAAAAAAG TCTGCCAGAT CGAGCTCATC 
CCCAAAGAGA AGATGGTGGC CCTGCTTTCT GGCCGTAATC GCCATGTCCA TTTATACCCC 
TGGGCGGCAC TAGAGGGCGC TGAAGGGGGT TTCGATGCCA AGCTGACAGA TACGAGAGGC 
TGCCAGGCCA TGACCATCGG TAACCTCCGC CCTGGCGGTC CAGCGTGTCT CTTTGCAGGC 
GTGAAGCGGC AGGTTTTCTG TTATGAAATC ACACGAGCGA AACCCCATCA TCGCAAACTC 
TGGGAAGTGC AGGCGCCAGG GGTCGTCCAA TGGCTGGGGA TAATTCGAGA ACGGATCTGC 
GTAGGTTACC CATCAGGCTT TGCTCTGCTG GCCATGCAGG GAGAATCGTC GCCGGTCAGT 
CTGGTGAGCC CAGGTGACCC CTCGCTGGCA TTTTTGGCCC AGCAGCCTCT GGATGCACTT 
CATGCTATGG AAGTGGGGTC CAATGAGCTG CTGCTGTGCT TCAGTCAGCT GGGAGTGTAC 
GTGGACGCGA CTGGAAGACG ATCACGAACT CAGGAGCTCA TGTGGCCCTC CACACCGCTC 
GCCTGCAGCT CAAACTCGAA CTGCCTGACG GTGTACAGCG ATTATGGGGT CGACGTGTTT 
GATGTTCACA CTATGGAATG GGTTCAGACC ATCTCATTAA GAAAAATTCG ACCTCTGAAT 
GTGGAAGGCA GTCTAAACCT TTTGAGCATT GAGCAACCCC ATCTCATCTA TTTCAGCAAC 
AACTCCTCGG ATGGCTGTGA CCTGGCCATC CCCGAGACCT CAGACAACAG CAAGAAGCTG 
ATGGTGAGGA CGCGCAGCAA GAGGAAGTTC CTTTTTAAAG TACCAGAGGA GGAGCGGCTG 
CAGCAGAGAA GGGAGATGCT GAGGGATCCT GAAATGAGAT CCAAGCTAAT CTCTCACCCC 
ACCAACTTCA ATCACGTTGC TCACATGGGC CCAGGCGACG GCATGCAGGT CCTCATGGAT 
CTGCCTCTGC CGAGCGAGAC TCCCTCCCCC TCATCCTCTC GCCACCATGC CCTGATCTCC 
CCTCCGACCA ACTTTGAGCA CGTCTATCAC ATGAGTCCCG TCTCTGCCGG GCTCTATCTG 
CAGAAAGAGC CTTCCTCCCA GCAGAGCCTC CTTCAGTTCT CCTCTTCCTC CTCCTCTCCT 
TCCACATCCT CCCTGGGAAG GGTAGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292898.1
CDS	858..5927
Translation	MSAKVRLKKLEQLLLDGHQKNDKSLSVETLLDILICLYNECSNSPLKREKHVTDFLEWVKPFTTTVKEMRLHRDDFEMLKVIGRGAFGEVAVVKMKHTERVYAMKILNKWEMLKRAETACFREERNVLVNGDCQWITTLHYAFQDDNYLYLVMDYYVGGDLLTLLSKFEDRLPEDMAKFYVAEMVLAIHSIHQQRYVHRDIKPDNVLLDMNGHIRLADFGSCLKMMQDGTVQSSVAVGTPDYISPEILQAMEDGMGKYGPECDWWSLGVCMYEMLYGETPFYAESLVETYGKIMNHEERFQFPSHITDVSEDAKDLIQRLICSRERRLGQHGIEEFRKHAFFSGIDWENIRNMEAPYIPDVSSPSDTSNFDVDDDVLKNPDIAPPVSHTGFTGQHLPFVGFTYTTESCFSDGGSMRGVALSDGGTGEDLQDGGMQVEAFEKRIRCLEQEKLELNRKLQESTQAVQSLHGSGRGAGTLGRDKEIKKLNEEIDRLKKKLADSDRLEHQLEEAVTLRQDFESSSTKLKALDKQVKALKLEKEDIHKQLVESLDRLKSQTKELKDAHQQRKLAMQEFSEVNERMAELRSQKQRLSRQLRDKEEEMEVVMQKIDAMRQDIRKTEKARKELESQLEDARAEASKERKLREHSEVYSKQLESELETLKVKQGAGRASGLGAETQQELTKLKSELDKKGLYYEEELVRREASHTTEMKNLRKELQDSEGQQLSLHKELLALKEKLEKAKRERQIEMEEAINALKEQYERERNLMSEDNRKLTAETDRLCSFVDKLTAQNRQLEDELQELASKKESVAHWEAQIAEIIQWVSDEKDARGYLQALASKMTEELESLRSSSLGSRTLDPLWKVRRSQKLDMSARLELQSALEAEIRAKQLVHDELRKVKAANISFESKLKDTEAKNRELEEQLENMKKEMEESRSRSDKGLKLPDFQDSIFDYFNTSPLAHELTFRDSVSSSSASSLLAFWDETSSVGDVDVAPQKTDIPPSSPSAATEQDEAVKPQATTPATPSPMYQPTLTAPKPKAHQLSIKTFSSPTQCTHCTSLMVGQMRQGYACEVCSFICHVSCKDNAPQLCPIPPEQTKRPLGIDVQRGIGTAYKGFVRIPKPTGVKKGWQRAYAVVCDCKLFLYEVPEGKSTQPVVMASQVLDLRDEEFSVSSVLASDVIHANHKDVPCIFRVTSSALKSSIRPVPLLILAESEAEKRKWVGILEGLQSILAKNRLKNRVVHVLHEAYDSSLPAIKTTLSAAIVDRERIVLGTEDGLFVVEITRDVIVRAADCKKVCQIELIPKEKMVALLSGRNRHVHLYPWAALEGAEGGFDAKLTDTRGCQAMTIGNLRPGGPACLFAGVKRQVFCYEITRAKPHHRKLWEVQAPGVVQWLGIIRERICVGYPSGFALLAMQGESSPVSLVSPGDPSLAFLAQQPLDALHAMEVGSNELLLCFSQLGVYVDATGRRSRTQELMWPSTPLACSSNSNCLTVYSDYGVDVFDVHTMEWVQTISLRKIRPLNVEGSLNLLSIEQPHLIYFSNNSSDGCDLAIPETSDNSKKLMVRTRSKRKFLFKVPEEERLQQRREMLRDPEMRSKLISHPTNFNHVAHMGPGDGMQVLMDLPLPSETPSPSSSRHHALISPPTNFEHVYHMSPVSAGLYLQKEPSSQQSLLQFSSSSSSPSTSSLGRVG

Target ORF information:

RefSeq Version	XM_009294623.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio CDC42 binding protein kinase beta (DMPK-like) (cdc42bpb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294623.2

ORF Insert Sequence:

ATGTCTGCGA AGGTGCGCCT GAAGAAGTTG GAGCAGTTGC TGCTGGATGG CCATCAGAAA 
AACGACAAGT CACTGAGTGT GGAGACACTG CTGGATATTC TCATATGTTT ATACAACGAG 
TGCAGCAACT CGCCCTTAAA ACGGGAAAAA CACGTGACGG ACTTTTTGGA ATGGGTGAAG 
CCGTTTACAA CCACTGTGAA AGAGATGCGC CTTCACAGAG ACGACTTCGA GATGCTGAAA 
GTGATCGGCA GGGGAGCCTT TGGAGAGGTT GCTGTGGTAA AGATGAAGCA CACAGAGCGG 
GTTTACGCCA TGAAGATCCT CAACAAGTGG GAGATGCTGA AAAGGGCTGA GACGGCATGC 
TTTCGGGAAG AGAGGAACGT CCTGGTGAAT GGAGACTGCC AGTGGATCAC TACCCTTCAT 
TATGCTTTTC AGGATGACAA CTATCTGTAC TTGGTGATGG ATTATTACGT TGGCGGTGAC 
CTGCTGACGC TGCTTAGTAA ATTTGAAGAC CGGCTGCCGG AGGACATGGC CAAGTTCTAC 
GTGGCGGAGA TGGTTCTCGC CATCCACTCC ATCCATCAGC AGCGCTACGT ACACAGGGAT 
ATAAAACCCG ACAACGTGCT GCTGGACATG AACGGCCACA TCCGACTCGC TGACTTCGGC 
TCCTGTCTGA AGATGATGCA AGACGGCACG GTCCAATCTT CAGTGGCTGT GGGCACACCA 
GACTACATCT CTCCAGAGAT CCTGCAGGCC ATGGAGGACG GCATGGGGAA ATACGGGCCG 
GAGTGTGACT GGTGGTCGCT GGGCGTTTGC ATGTATGAGA TGCTGTACGG CGAGACACCC 
TTTTACGCCG AATCCCTGGT GGAGACCTAT GGAAAGATCA TGAACCATGA GGAGCGTTTC 
CAGTTCCCTT CCCACATCAC GGATGTATCC GAGGATGCTA AAGACCTGAT CCAGAGGCTG 
ATTTGCAGCC GAGAGCGCCG TTTGGGTCAA CATGGCATCG AGGAGTTCAG GAAGCACGCC 
TTTTTCTCCG GCATCGACTG GGAGAACATC CGGAACATGG AGGCGCCATA CATCCCTGAC 
GTCAGCTCTC CATCAGACAC CTCCAACTTT GATGTAGATG ATGACGTACT GAAGAATCCA 
GACATTGCTC CTCCAGTTTC TCACACGGGT TTCACCGGGC AGCACTTGCC CTTTGTGGGC 
TTCACCTACA CTACAGAGAG CTGTTTCTCA GACGGCGGCA GCATGAGGGG TGTGGCGCTG 
TCTGACGGGG GCACCGGGGA GGATCTCCAG GATGGAGGCA TGCAGGTGGA GGCCTTTGAG 
AAGAGGATTC GCTGCCTGGA GCAAGAGAAA TTGGAGCTTA ACCGTAAGCT GCAAGAATCC 
ACTCAGGCTG TTCAATCTCT TCATGGCTCT GGTCGAGGAG CCGGCACTCT CGGACGAGAT 
AAAGAGATTA AAAAACTTAA TGAGGAAATC GATCGGCTTA AAAAGAAACT GGCAGACTCT 
GATAGGCTGG AGCACCAGTT GGAGGAAGCA GTGACTCTTC GACAGGACTT TGAAAGTTCC 
TCCACCAAAC TAAAAGCCCT GGACAAGCAA GTCAAAGCTC TCAAGCTGGA GAAAGAGGAT 
ATTCATAAGC AACTGGTGGA GTCTTTGGAC CGCCTGAAGT CTCAGACCAA GGAGCTGAAG 
GACGCGCACC AGCAGAGGAA GCTGGCCATG CAGGAGTTCT CGGAGGTGAA CGAGCGTATG 
GCCGAACTGC GCTCCCAGAA GCAGCGTCTG TCGCGCCAGC TGCGGGACAA GGAGGAGGAG 
ATGGAGGTGG TGATGCAGAA AATCGACGCC ATGCGCCAGG ACATCCGCAA AACCGAGAAG 
GCCCGCAAGG AGCTGGAGTC TCAGCTGGAG GATGCGCGTG CCGAAGCTTC TAAGGAGCGT 
AAACTTCGAG AGCACAGTGA GGTTTACTCC AAACAACTGG AGAGTGAACT GGAGACACTC 
AAGGTGAAGC AGGGCGCAGG TCGTGCGTCA GGCCTCGGTG CAGAGACTCA GCAGGAGCTC 
ACCAAACTCA AGTCTGAGCT AGATAAGAAA GGACTGTATT ATGAGGAAGA GCTGGTGAGA 
CGTGAAGCCA GTCACACTAC AGAGATGAAA AACCTGAGGA AGGAGCTCCA GGACTCTGAG 
GGTCAGCAGC TCTCCCTGCA CAAAGAGCTG CTTGCGCTTA AAGAAAAACT GGAAAAGGCC 
AAGAGAGAAC GGCAAATTGA GATGGAAGAG GCCATTAATG CCCTGAAAGA GCAGTATGAA 
CGTGAACGCA ACCTTATGAG TGAAGACAAC CGCAAACTCA CTGCAGAAAC AGACCGGCTC 
TGCAGTTTTG TGGACAAGCT GACCGCTCAG AACAGGCAAC TGGAGGACGA GCTCCAGGAA 
CTGGCATCAA AGAAGGAGAG CGTAGCACAC TGGGAAGCAC AGATCGCAGA GATTATCCAG 
TGGGTGAGCG ATGAGAAAGA CGCACGCGGC TACCTTCAGG CTCTGGCCTC CAAGATGACA 
GAAGAGTTGG AGTCTCTGCG CAGCTCCAGT TTGGGATCCA GAACACTGGA TCCGTTGTGG 
AAGGTGCGTC GCAGTCAAAA GCTGGATATG TCTGCCCGTC TAGAGCTGCA GTCGGCCTTG 
GAAGCTGAAA TCCGTGCCAA GCAACTGGTT CACGATGAAC TGCGCAAGGT CAAAGCTGCC 
AACATCTCTT TCGAGAGCAA ACTGAAGGAC ACCGAGGCCA AGAACAGAGA GTTGGAGGAA 
CAGCTGGAGA ACATGAAGAA GGAGATGGAG GAGAGCCGCT CACGATCAGA CAAAGGCCTG 
AAACTGCCAG ACTTCCAGGA CTCCATCTTT GACTACTTCA ATACCTCTCC TCTTGCCCAT 
GAGCTGACAT TCAGAGACTC TGTCTCCTCC TCATCTGCAT CTTCTCTGCT TGCATTTTGG 
GACGAAACCA GCTCTGTCGG AGATGTGGAC GTCGCACCTC AGAAGACTGA CATTCCCCCT 
TCCTCCCCAT CAGCAGCTAC AGAACAGGAT GAAGCAGTCA AGCCTCAAGC GACCACTCCT 
GCTACTCCTT CTCCAATGTA TCAGCCCACT CTCACTGCAC CAAAGCCCAA AGCCCATCAG 
CTGAGCATCA AGACCTTCTC CAGCCCGACT CAGTGCACTC ACTGTACTTC ACTGATGGTG 
GGCCAGATGC GTCAAGGATA CGCCTGCGAG GTATGCTCTT TCATCTGCCA CGTGTCCTGT 
AAAGACAACG CACCCCAGCT CTGCCCCATT CCTCCCGAGC AGACCAAGAG GCCTCTGGGT 
ATTGATGTGC AGAGGGGCAT TGGCACTGCG TACAAAGGTT TTGTCAGGAT CCCGAAGCCC 
ACCGGAGTGA AGAAGGGCTG GCAGAGAGCA TATGCTGTAG TGTGTGACTG TAAACTCTTT 
CTCTACGAGG TGCCAGAAGG GAAGTCCACT CAACCTGTCG TGATGGCCAG TCAGGTCCTT 
GACTTGAGGG ATGAGGAGTT CTCTGTTAGC TCTGTGTTGG CATCTGATGT GATCCATGCC 
AACCATAAAG ACGTTCCCTG TATATTCAGG GTAACCTCGT CGGCTCTGAA ATCCTCGATC 
CGGCCAGTTC CTTTGCTGAT TTTAGCTGAG AGCGAGGCGG AGAAGAGGAA GTGGGTGGGC 
ATCCTAGAGG GCCTGCAGAG CATCCTAGCC AAAAACCGGC TGAAGAACCG CGTGGTGCAT 
GTGCTGCACG AGGCCTACGA CAGCAGCCTA CCTGCCATCA AAACTACACT TTCTGCTGCC 
ATCGTAGATC GAGAACGAAT TGTCTTGGGG ACAGAAGATG GCCTTTTTGT AGTCGAGATC 
ACCAGAGATG TCATTGTTCG TGCCGCTGAC TGTAAAAAAG TCTGCCAGAT CGAGCTCATC 
CCCAAAGAGA AGATGGTGGC CCTGCTTTCT GGCCGTAATC GCCATGTCCA TTTATACCCC 
TGGGCGGCAC TAGAGGGCGC TGAAGGGGGT TTCGATGCCA AGCTGACAGA TACGAGAGGC 
TGCCAGGCCA TGACCATCGG TAACCTCCGC CCTGGCGGTC CAGCGTGTCT CTTTGCAGGC 
GTGAAGCGGC AGGTTTTCTG TTATGAAATC ACACGAGCGA AACCCCATCA TCGCAAACTC 
TGGGAAGTGC AGGCGCCAGG GGTCGTCCAA TGGCTGGGGA TAATTCGAGA ACGGATCTGC 
GTAGGTTACC CATCAGGCTT TGCTCTGCTG GCCATGCAGG GAGAATCGTC GCCGGTCAGT 
CTGGTGAGCC CAGGTGACCC CTCGCTGGCA TTTTTGGCCC AGCAGCCTCT GGATGCACTT 
CATGCTATGG AAGTGGGGTC CAATGAGCTG CTGCTGTGCT TCAGTCAGCT GGGAGTGTAC 
GTGGACGCGA CTGGAAGACG ATCACGAACT CAGGAGCTCA TGTGGCCCTC CACACCGCTC 
GCCTGCAGCT CAAACTCGAA CTGCCTGACG GTGTACAGCG ATTATGGGGT CGACGTGTTT 
GATGTTCACA CTATGGAATG GGTTCAGACC ATCTCATTAA GAAAAATTCG ACCTCTGAAT 
GTGGAAGGCA GTCTAAACCT TTTGAGCATT GAGCAACCCC ATCTCATCTA TTTCAGCAAC 
AACTCCTCGG ATGGCTGTGA CCTGGCCATC CCCGAGACCT CAGACAACAG CAAGAAGCTG 
ATGGTGAGGA CGCGCAGCAA GAGGAAGTTC CTTTTTAAAG TACCAGAGGA GGAGCGGCTG 
CAGCAGAGAA GGGAGATGCT GAGGGATCCT GAAATGAGAT CCAAGCTAAT CTCTCACCCC 
ACCAACTTCA ATCACGTTGC TCACATGGGC CCAGGCGACG GCATGCAGGT CCTCATGGAT 
CTGCCTCTGC CGAGCGAGAC TCCCTCCCCC TCATCCTCTC GCCACCATGC CCTGATCTCC 
CCTCCGACCA ACTTTGAGCA CGTCTATCAC ATGAGTCCCG TCTCTGCCGG GCTCTATCTG 
CAGAAAGAGC CTTCCTCCCA GCAGAGCCTC CTTCAGTTCT CCTCTTCCTC CTCCTCTCCT 
TCCACATCCT CCCTGGGAAG GGTAGGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The zebrafish gene map defines ancestral vertebrate chromosomes.
Genome research15(9)1307-14(2005 Sep)
Woods IG,Wilson C,Friedlander B,Chang P,Reyes DK,Nix R,Kelly PD,Chu F,Postlethwait JH,Talbot WS

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

Expression profiling and comparative genomics identify a conserved regulatory region controlling midline expression in the zebrafish embryo.
Genome research14(2)228-38(2004 Feb)
Dickmeis T,Plessy C,Rastegar S,Aanstad P,Herwig R,Chalmel F,Fischer N,Str?hle U

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CDC42BPB cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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