sfrp2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	sfrp2
Entrez Gene ID	566878
Full Name	secreted frizzled-related protein 2
Synonyms	hm:zeh0225,sb:eu233,zeh0225,zgc:153618
General protein information	Preferred Names secreted frizzled-related protein 2 Names secreted frizzled-related protein 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001077384.2	NP_001070852.2	secreted frizzled-related protein 2 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SFRP2	XP_001155803.1
Mus musculus (house mouse)	Sfrp2	NP_033170.1
Rattus norvegicus (Norway rat)	Sfrp2	NP_001094170.1
Homo sapiens (human)	SFRP2	NP_003004.1
Xenopus tropicalis (tropical clawed frog)	sfrp2	NP_989062.1
Danio rerio (zebrafish)	sfrp2	NP_001070852.2
Bos taurus (cattle)	SFRP2	NP_001029565.1
Caenorhabditis elegans (roundworm)	sfrp-1	NP_500977.2
Canis lupus familiaris (dog)	SFRP2	NP_001002987.1
Gallus gallus (chicken)	SFRP2	NP_990104.1

Gene Ontology
Bibliography

Related articles in PubMed

Engrailed controls epaxial-hypaxial muscle innervation and the establishment of vertebrate three-dimensional mobility.
Ahmed MU, Maurya AK, Cheng L, Jorge EC, Schubert FR, Maire P, Basson MA, Ingham PW, Dietrich S
Developmental biology430(1)90-104(2017 10)

Genome-wide analysis of facial skeletal regionalization in zebrafish.
Askary A, Xu P, Barske L, Bay M, Bump P, Balczerski B, Bonaguidi MA, Crump JG
Development (Cambridge, England)144(16)2994-3005(2017 08)

In Vivo Characterization of an AHR-Dependent Long Noncoding RNA Required for Proper
Garcia GR, Goodale BC, Wiley MW, La Du JK, Hendrix DA, Tanguay RL
Molecular pharmacology91(6)609-619(2017 Jun)

Transcriptomic, proteomic, and metabolomic landscape of positional memory in the caudal fin of zebrafish.
Rabinowitz JS, Robitaille AM, Wang Y, Ray CA, Thummel R, Gu H, Djukovic D, Raftery D, Berndt JD, Moon RT
Proceedings of the National Academy of Sciences of the United States of America114(5)E717-E726(2017 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

cysteine-rich domain of sFRP2 is sufficient for Ror2 activation, and related sFRPs can substitute for this function. Notably, direct interaction of the two receptors via their cysteine-rich domains also promotes Ror2-mediated papc expression but inhibits Fz7 signaling.
Title: Secreted Frizzled-related Protein 2 (sFRP2) Redirects Non-canonical Wnt Signaling from Fz7 to Ror2 during Vertebrate Gastrulation.

The following sfrp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the sfrp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa01504	NM_001077384.2 Latest version!	Danio rerio secreted frizzled-related protein 2 (sfrp2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

sfrp2 ( NM_001077384.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001070852.2 Danio rerio secreted frizzled-related protein 2 (sfrp2), mRNA.

Price & Availability↑

CloneID	ODa01504
Clone ID Related Accession (Same CDS sequence)	NM_001077384.2
Accession Version	NM_001077384.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-07
Organism	Danio rerio(zebrafish)
Product	secreted frizzled-related protein 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX950209.14. On Jun 13, 2009 this sequence version replaced NM_001077384.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC124707.1, GFIL01034987.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGCGTGCCT ATCTGGCTCT GCTGGTTGCG CTTTCCATCC CGGGATATCT GGATGCGCTT 
CACGGATTAT ACGCCTTCGA TCAGCCTGAG CTCTTTCAGA AAAAGAGCAA CTGTAAGCCC 
ATACCTACAA ACCTGCTCCT GTGCCATGAC ATCGAGTACA CAAACATGCG TCTGCCTAAC 
CTCCTCGGAC ACGAGACCAT GAACGAGGTT CTGCAACAAG CATCCTCCTG GACCCCTCTG 
GTGCAGAAAC AGTGTCATCC GGACACCAAG AAGTTTCTCT GCTCTCTCTT CGCTCCGGTG 
TGTTTGGATG ACCTGGACGA GCCCATCCAG CCGTGCAGGT CTCTGTGTGA GAGCGTGAAG 
AGCGGCTGCG CTCCGGTGAT GGCCGCTTTT GGGTTCCCTT GGCCGGACAT GCTGGACTGC 
AGGCGCTTCC CTTTAGATAA TGACCTGTGC ATACCGCCTG CTGGCGCTGA TGCTTTAGTA 
CCAGTCACTA AGGAAGTTCC AAGAGTATGT GATGCCTGCA AGGAAAAGCC TGAAAATGAC 
AACGAAATTG TGGACAGCCT ATGCAAAAAT GACTTTGCTT TAAAGATCAA AGTCAAGGAG 
ATCTCCTACA TGAATGGTGA CACCAAGATC ATTCCAGAAA CCAAGACCAA GATGATCTAC 
AAGACGAATG GTGGCGTCAC TGACCATGAC TTTAGGAAGA CGGTGCTTTG GCTAAAAGAT 
GGTCTTCAGT GTGTGTGCGA TGAACTAAAT GACATCAATG CTGCTTATTT GGTGATGGGC 
CAGAAGATGG ATGGAAATTT GGTCATCACA TCGGTGAAAC GCTGGCAGAA GGGTCAACGA 
GAGTTTAAGA GAATTTCTCG CAGCATTCGC AAACTTCAGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001070852.2
CDS	32..916
Translation	MRAYLALLVALSIPGYLDALHGLYAFDQPELFQKKSNCKPIPTNLLLCHDIEYTNMRLPNLLGHETMNEVLQQASSWTPLVQKQCHPDTKKFLCSLFAPVCLDDLDEPIQPCRSLCESVKSGCAPVMAAFGFPWPDMLDCRRFPLDNDLCIPPAGADALVPVTKEVPRVCDACKEKPENDNEIVDSLCKNDFALKIKVKEISYMNGDTKIIPETKTKMIYKTNGGVTDHDFRKTVLWLKDGLQCVCDELNDINAAYLVMGQKMDGNLVITSVKRWQKGQREFKRISRSIRKLQC

Target ORF information:

RefSeq Version	NM_001077384.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio secreted frizzled-related protein 2 (sfrp2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001077384.2

ORF Insert Sequence:

ATGCGTGCCT ATCTGGCTCT GCTGGTTGCG CTTTCCATCC CGGGATATCT GGATGCGCTT 
CACGGATTAT ACGCCTTCGA TCAGCCTGAG CTCTTTCAGA AAAAGAGCAA CTGTAAGCCC 
ATACCTACAA ACCTGCTCCT GTGCCATGAC ATCGAGTACA CAAACATGCG TCTGCCTAAC 
CTCCTCGGAC ACGAGACCAT GAACGAGGTT CTGCAACAAG CATCCTCCTG GACCCCTCTG 
GTGCAGAAAC AGTGTCATCC GGACACCAAG AAGTTTCTCT GCTCTCTCTT CGCTCCGGTG 
TGTTTGGATG ACCTGGACGA GCCCATCCAG CCGTGCAGGT CTCTGTGTGA GAGCGTGAAG 
AGCGGCTGCG CTCCGGTGAT GGCCGCTTTT GGGTTCCCTT GGCCGGACAT GCTGGACTGC 
AGGCGCTTCC CTTTAGATAA TGACCTGTGC ATACCGCCTG CTGGCGCTGA TGCTTTAGTA 
CCAGTCACTA AGGAAGTTCC AAGAGTATGT GATGCCTGCA AGGAAAAGCC TGAAAATGAC 
AACGAAATTG TGGACAGCCT ATGCAAAAAT GACTTTGCTT TAAAGATCAA AGTCAAGGAG 
ATCTCCTACA TGAATGGTGA CACCAAGATC ATTCCAGAAA CCAAGACCAA GATGATCTAC 
AAGACGAATG GTGGCGTCAC TGACCATGAC TTTAGGAAGA CGGTGCTTTG GCTAAAAGAT 
GGTCTTCAGT GTGTGTGCGA TGAACTAAAT GACATCAATG CTGCTTATTT GGTGATGGGC 
CAGAAGATGG ATGGAAATTT GGTCATCACA TCGGTGAAAC GCTGGCAGAA GGGTCAACGA 
GAGTTTAAGA GAATTTCTCG CAGCATTCGC AAACTTCAGT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Engrailed controls epaxial-hypaxial muscle innervation and the establishment of vertebrate three-dimensional mobility.
Developmental biology430(1)90-104(2017 10)
Ahmed MU,Maurya AK,Cheng L,Jorge EC,Schubert FR,Maire P,Basson MA,Ingham PW,Dietrich S

Genome-wide analysis of facial skeletal regionalization in zebrafish.
Development (Cambridge, England)144(16)2994-3005(2017 08)
Askary A,Xu P,Barske L,Bay M,Bump P,Balczerski B,Bonaguidi MA,Crump JG

In Vivo Characterization of an AHR-Dependent Long Noncoding RNA Required for Proper
Molecular pharmacology91(6)609-619(2017 Jun)
Garcia GR,Goodale BC,Wiley MW,La Du JK,Hendrix DA,Tanguay RL

Transcriptomic, proteomic, and metabolomic landscape of positional memory in the caudal fin of zebrafish.
Proceedings of the National Academy of Sciences of the United States of America114(5)E717-E726(2017 Jan)
Rabinowitz JS,Robitaille AM,Wang Y,Ray CA,Thummel R,Gu H,Djukovic D,Raftery D,Berndt JD,Moon RT

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sfrp2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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