mical3b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mical3b
Entrez Gene ID	566850
Full Name	microtubule associated monooxygenase, calponin and LIM domain containing 3b
Synonyms	MICAL-3B,im:7143388,si:dkey-153k10.11,si:dkeyp-27e10.1
General protein information	Preferred Names microtubule associated monooxygenase, calponin and LIM domain containing 3b Names protein-methionine sulfoxide oxidase mical3b molecule interacting with CasL 3b molecule interacting with CasL protein 3B
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021473992.1	XP_021329667.1	protein-methionine sulfoxide oxidase mical3b isoform X1
XM_009300192.2	XP_009298467.1	protein-methionine sulfoxide oxidase mical3b isoform X6
NM_001353876.1	NP_001340805.1	protein-methionine sulfoxide oxidase mical3b
XM_017355273.1	XP_017210762.1	protein-methionine sulfoxide oxidase mical3b isoform X1
XM_017355277.1	XP_017210766.1	protein-methionine sulfoxide oxidase mical3b isoform X3
XM_017355278.1	XP_017210767.1	protein-methionine sulfoxide oxidase mical3b isoform X4
XM_017355280.1	XP_017210769.1	protein-methionine sulfoxide oxidase mical3b isoform X7
XM_009300195.2	XP_009298470.1	protein-methionine sulfoxide oxidase mical3b isoform X8
XM_017355279.2	XP_017210768.1	protein-methionine sulfoxide oxidase mical3b isoform X1
XM_021475218.1	XP_021330893.1	protein-methionine sulfoxide oxidase mical3b isoform X7
XM_017355279.1	XP_017210768.1	protein-methionine sulfoxide oxidase mical3b isoform X5
XM_021471526.1	XP_021327201.1	protein-methionine sulfoxide oxidase mical3b isoform X9
XM_021473993.1	XP_021329668.1	protein-methionine sulfoxide oxidase mical3b isoform X2
XM_021473997.1	XP_021329672.1	protein-methionine sulfoxide oxidase mical3b isoform X6
XM_021473998.1	XP_021329673.1	protein-methionine sulfoxide oxidase mical3b isoform X7
XM_009300192.3	XP_009298467.1	protein-methionine sulfoxide oxidase mical3b isoform X4
XM_021475217.1	XP_021330892.1	protein-methionine sulfoxide oxidase mical3b isoform X5
XM_017355274.1	XP_017210763.1	protein-methionine sulfoxide oxidase mical3b isoform X1
XM_017355276.1	XP_017210765.1	protein-methionine sulfoxide oxidase mical3b isoform X2
XM_017355281.1	XP_017210770.1	protein-methionine sulfoxide oxidase mical3b isoform X9
XM_021475214.1	XP_021330889.1	protein-methionine sulfoxide oxidase mical3b isoform X1
XM_021475216.1	XP_021330891.1	protein-methionine sulfoxide oxidase mical3b isoform X3
XM_009300195.3	XP_009298470.1	protein-methionine sulfoxide oxidase mical3b isoform X6
XM_021475219.1	XP_021330894.1	protein-methionine sulfoxide oxidase mical3b isoform X8
XM_021473994.1	XP_021329669.1	protein-methionine sulfoxide oxidase mical3b isoform X3
XM_021473995.1	XP_021329670.1	protein-methionine sulfoxide oxidase mical3b isoform X4
NM_001353875.1	NP_001340804.1	protein-methionine sulfoxide oxidase mical3b
XM_021475215.1	XP_021330890.1	protein-methionine sulfoxide oxidase mical3b isoform X2
XM_021473996.1	XP_021329671.1	protein-methionine sulfoxide oxidase mical3b isoform X5

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Eumetazoan cryptochrome phylogeny and evolution.
Haug MF, Gesemann M, Lazovi? V, Neuhauss SC
Genome biology and evolution7(2)601-19(2015 Jan)

Functional validation of GWAS gene candidates for abnormal liver function during zebrafish liver development.
Liu LY, Fox CS, North TE, Goessling W
Disease models & mechanisms6(5)1271-8(2013 Sep)

Identification and expression analysis of mical family genes in zebrafish.
Xue Y, Kuok C, Xiao A, Zhu Z, Lin S, Zhang B
Journal of genetics and genomics = Yi chuan xue bao37(10)685-93(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mical3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mical3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa142127	XM_021475219.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa44979	NM_001353876.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa44979	NM_001353875.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa142123	XM_021475215.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa142124	XM_021475216.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa142125	XM_021475217.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa142126	XM_021475218.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa146493	XM_021473992.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa146494	XM_021473993.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa146495	XM_021473994.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa146496	XM_021473995.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa146497	XM_021473996.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa146498	XM_021473997.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa146499	XM_021473998.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
ODa44975	XM_009300192.3 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa44977	XM_009300195.3 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa58545	XM_017355274.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa58545	XM_017355273.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa58546	XM_017355276.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa58547	XM_017355277.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa58548	XM_017355278.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa58549	XM_021475214.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa58549	XM_017355279.2 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa58550	XM_017355280.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa58551	XM_017355281.1 Latest version!	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
View Old Accession Versions >>
ODa58549	XM_017355279.1	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa44975	XM_009300192.2	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa44977	XM_009300195.2	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mical3b ( XM_021475219.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330894.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X9, mRNA.

Price & Availability↑

CloneID	ODa142127
Clone ID Related Accession (Same CDS sequence)	XM_021475219.1
Accession Version	XM_021475219.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGAGGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAG AAATCATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330894.1
CDS	205..3306
Translation	MWDGQSEMCQAHVLFDSFVQAATCKETLRAFQDLCEELNLNPGGQPQFYHTLRSRLHYWKAKALWAKLDKRACQKEYMRGHACTSTTCLIIGAGPCGLRTAIELGFLGARVVLVEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRKVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTAIRRQNYIHMYTGGVSSLALQIANQIQSQQAQAPKLLHRRESGSQKDLPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSSESDLEEEELVWKKGEELHARTNGERKLDLEEELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDEKSS

Target ORF information:

RefSeq Version	XM_021475219.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475219.1

ORF Insert Sequence:

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGAGGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAG AAATCATCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( NM_001353876.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001340805.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), mRNA.

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CloneID	ODa44979
Clone ID Related Accession (Same CDS sequence)	NM_001353876.1 , NM_001353875.1
Accession Version	NM_001353876.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX649444.12 and BX901962.8. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01026062.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
GGTTCTCAGA AGGATCTTCC AGTAAATGTC GGCAGTAGTG ATGTGTGTTA TTTCTGTGGC 
CGTCGCGTTT ATGTCATGGA GAGGCTGAGC GCAGAGGGAA AGTTCTTCCA CAGGAGCTGC 
TTCCAGTGCG ACCACTGCAG CTCCACGATA CGCCTCTCCA ACTACACTTA TGACCAGCTA 
CATGGCAAGT TTTACTGTAA GCATCACTTC AGCTTCAGAC TGGCAAGTGT GGCTCAGAGA 
AAGAGACCGG CCCCACCGGT GGCCCCTCGG CCTGCTCAGG CTTCCCTGGC TGCATCCTCG 
GCCTCTACCT CTTTGTCCTC TTTGGGCTCA GTGGGCACAG CCACACCAGA CAGCTGGTCC 
TCCAGCACCC ACACAGACAT GGCATCCAGC TTGGCCAAGA GACTGTGCGG CACACCAGAA 
CGCATTGAGC TTGAGAATTA CAAACCCTCC CCACAGAAAC AAGACAGCCC CCTGCAGGAG 
GTACCAGAGG AAACCCTCGC ACAGCACAAT CTCAGCGCTA GCCTGCAGGA GAAGAATGCT 
GAAGAGCAGT CCAGTAGCTC TGAGTCAGAT CTAGAGGAGG AGGAGCTTGT CTGGAAGAAG 
GGGGAGGAGC TACATGCCAG GACCAATGGA GAGAGGAAGC TTGACCTAGA GGAGGAGCTG 
AAGGAAGAAG AGGGAGGGGA GAAGCTGGAA AAACAAGAGG GGGAGGAAGA AGGAGAAGTT 
TCAGAAGAAG AGCAAGACGA GGGAGATTCT AGTGATGAGG GTGAGTACTG CCCTTGGGAG 
AGGGAACGAC TCTCAGGGGT GTGGCTTGAG GAAGAGGAGG CGGGGTTTGT TAAAGAATCT 
TATGAAGGAT GCAGCGACGA CCCTGATATA GACGTCAGCT CACTCAGTGA GTCCAAGCTA 
TGTGACCAAC GGGAACAGGA GGAGTCTGTT CCTTTCCACC AATCCCCTGC CTCTACAGAA 
TCTCCAGTCT CAATGAAACC TTCAGAATCA GACCTCACAC CTGATCCGTC AACAACCCCC 
GAGAGCTCGC CTGCCAAGAG GTCAGAGGTG GTGGAGGAGT TCTGGATGAA GAGTGCAGAG 
ATCCGAAAGA GTTTAGGCCT GACTCCGCTT TCTAGAGAAT TGCAGCCAAA ACATATAGCA 
GCTTCAACCC AGACATCTAA TGTTAAAGAA AGCTTTTACA CCTCAGTCAC ATATAACACC 
GCTGCTTTGG ATTCTCCAAA CCAAAGTGCT AGAATCTGTG ACTCCAGCAC ACAAACTCAT 
TCTGTGACAG ATCTTCAAGA AACCTCCCCT CTTGGACCTA CAGATGGCGA TGCTGGCGTC 
GATTTAGGAC GTTGTTCTGT TGTTCACAGA CTCAGCATCA CTGTAGAAGG TTGTGTGATG 
GCAGATAACC AGGGTTTGGA TTCTACCTCG TTTGCTACTG AAAGTGTTTT AAACCCAGAC 
GCAGGTCTTC CCACCCCTCC TTATAGTCCG TCTCACTCCC CTCTTGTTGG TAAACAGTGT 
CGTGCCCTTC ACCATTCTGA GCCAATTCTA GACAGAGAGG TTATGGCTTT CTCATCTACC 
TGCACTGCTT CTGTACCACA GCGGAGCAGT TTTAAATCAG ACCTCGAGCA AGCTCAAAGT 
CTTCCACCAG ATGAAATCGA GATCCTGTGC GGAGACGAGG CGGAAAAACT CCCGGAAAGA 
TCTTGCAGGA TGGAGAGCGG AGAGGTGGAT AACCGTAGAG CGGAGCACAG CAGGACGCTT 
CCTGACCGGG TGGTCGCACC GCTGCTGGCA GGGGGACCAG AGGTCAGGCT TCGCAGGTCT 
GAGATGAAGT TGTGGGGTCC AGACGCAGAT GGTGATGTGA AGGAGAAAAA GCGCAGCTCG 
TTGTTTTCAC CCCGTAAAAG CAGGAAGAAT GGAAATGCAG CAGCTGAGAG CGGGCGAGAG 
ACAGGGAAGC ACAAGTCCCT CTGGAAGACT GTTTTTTCAG TGTATAAGAA GGATAAAAAG 
AGGAAGGAGG TGGCAGTGGT GGCGGAAACA CTTCCTGCTG CTAATAATGG GAGCAAACGG 
AAAGTGTCGG GAATTAACAG AACCACAGAC CTGTGTTTCC GGAAGAATCC AAGTTTCTCT 
GAAGATACGG ATATGTCCTG TCATGCTCTT CTTGAAAGAT GTCCTCTCCG AGCTCAGAGA 
GCAGGAACAG AGGAGGAACT CAATGCCAGG CTGACACGTC GAGTGCAGAG AGCAGCGCGC 
AGACAGGCCA AGCAGGAGGA GCTGCGGAGG CTGCATAGAG CTCAGATAAT CCAGCGTCAG 
TTGGAGCAGG TGGAAGTGAA GCAGAGGCAG CTGGAGGAGA AAGGTGTGGC TGTGGAGAAA 
GCACTCAGAG GAGAAGCAGA CTTCTGGGAA GACTCTAGTA CCTCTGTCCT CTTGGATGTA 
CATCTGTGTG GAATGGGAAA GAAAGACGAT CCAAGCTTAA TGCATCAGTG GTTCAAACTG 
GTGCAGGAGA AGAATGCTCT CGTGCGTTAT GAATCTGAGC TCATGATATT CGCTCGTGAA 
CTGGAGCTGG AGGACAGACA GAGTCGCCTG CAGCAGGAGC TGAGAGAGCG CATGGCGGTG 
GACGATCATC TAAAAGGAGA AGAGGAGCTG GCGGAGGAGA GGCGTATATT GAGCGAGATG 
CTGGATGTGG TGGAGCAGAG GGATGCACTG GTGGCCCTCC TGGAGGAGCA GAGAGTTCGA 
GAAAAAGAGG AGGACAGTGA CCTGGAGGCT GTGATGCTGT CTAAGGGCTT CAGTCTGCAT 
TGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340805.1
CDS	232..5220
Translation	MWDGQSEMCQAHVLFDSFVQAATCKETLRAFQDLCEELNLNPGGQPQFYHTLRSRLHYWKAKALWAKLDKRACQKEYMRGHACTSTTCLIIGAGPCGLRTAIELGFLGARVVLVEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRKVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTAIRRQGSQKDLPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSSESDLEEEELVWKKGEELHARTNGERKLDLEEELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDEGEYCPWERERLSGVWLEEEEAGFVKESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNGSKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEADFWEDSSTSVLLDVHLCGMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	NM_001353876.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353876.1

ORF Insert Sequence:

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
GGTTCTCAGA AGGATCTTCC AGTAAATGTC GGCAGTAGTG ATGTGTGTTA TTTCTGTGGC 
CGTCGCGTTT ATGTCATGGA GAGGCTGAGC GCAGAGGGAA AGTTCTTCCA CAGGAGCTGC 
TTCCAGTGCG ACCACTGCAG CTCCACGATA CGCCTCTCCA ACTACACTTA TGACCAGCTA 
CATGGCAAGT TTTACTGTAA GCATCACTTC AGCTTCAGAC TGGCAAGTGT GGCTCAGAGA 
AAGAGACCGG CCCCACCGGT GGCCCCTCGG CCTGCTCAGG CTTCCCTGGC TGCATCCTCG 
GCCTCTACCT CTTTGTCCTC TTTGGGCTCA GTGGGCACAG CCACACCAGA CAGCTGGTCC 
TCCAGCACCC ACACAGACAT GGCATCCAGC TTGGCCAAGA GACTGTGCGG CACACCAGAA 
CGCATTGAGC TTGAGAATTA CAAACCCTCC CCACAGAAAC AAGACAGCCC CCTGCAGGAG 
GTACCAGAGG AAACCCTCGC ACAGCACAAT CTCAGCGCTA GCCTGCAGGA GAAGAATGCT 
GAAGAGCAGT CCAGTAGCTC TGAGTCAGAT CTAGAGGAGG AGGAGCTTGT CTGGAAGAAG 
GGGGAGGAGC TACATGCCAG GACCAATGGA GAGAGGAAGC TTGACCTAGA GGAGGAGCTG 
AAGGAAGAAG AGGGAGGGGA GAAGCTGGAA AAACAAGAGG GGGAGGAAGA AGGAGAAGTT 
TCAGAAGAAG AGCAAGACGA GGGAGATTCT AGTGATGAGG GTGAGTACTG CCCTTGGGAG 
AGGGAACGAC TCTCAGGGGT GTGGCTTGAG GAAGAGGAGG CGGGGTTTGT TAAAGAATCT 
TATGAAGGAT GCAGCGACGA CCCTGATATA GACGTCAGCT CACTCAGTGA GTCCAAGCTA 
TGTGACCAAC GGGAACAGGA GGAGTCTGTT CCTTTCCACC AATCCCCTGC CTCTACAGAA 
TCTCCAGTCT CAATGAAACC TTCAGAATCA GACCTCACAC CTGATCCGTC AACAACCCCC 
GAGAGCTCGC CTGCCAAGAG GTCAGAGGTG GTGGAGGAGT TCTGGATGAA GAGTGCAGAG 
ATCCGAAAGA GTTTAGGCCT GACTCCGCTT TCTAGAGAAT TGCAGCCAAA ACATATAGCA 
GCTTCAACCC AGACATCTAA TGTTAAAGAA AGCTTTTACA CCTCAGTCAC ATATAACACC 
GCTGCTTTGG ATTCTCCAAA CCAAAGTGCT AGAATCTGTG ACTCCAGCAC ACAAACTCAT 
TCTGTGACAG ATCTTCAAGA AACCTCCCCT CTTGGACCTA CAGATGGCGA TGCTGGCGTC 
GATTTAGGAC GTTGTTCTGT TGTTCACAGA CTCAGCATCA CTGTAGAAGG TTGTGTGATG 
GCAGATAACC AGGGTTTGGA TTCTACCTCG TTTGCTACTG AAAGTGTTTT AAACCCAGAC 
GCAGGTCTTC CCACCCCTCC TTATAGTCCG TCTCACTCCC CTCTTGTTGG TAAACAGTGT 
CGTGCCCTTC ACCATTCTGA GCCAATTCTA GACAGAGAGG TTATGGCTTT CTCATCTACC 
TGCACTGCTT CTGTACCACA GCGGAGCAGT TTTAAATCAG ACCTCGAGCA AGCTCAAAGT 
CTTCCACCAG ATGAAATCGA GATCCTGTGC GGAGACGAGG CGGAAAAACT CCCGGAAAGA 
TCTTGCAGGA TGGAGAGCGG AGAGGTGGAT AACCGTAGAG CGGAGCACAG CAGGACGCTT 
CCTGACCGGG TGGTCGCACC GCTGCTGGCA GGGGGACCAG AGGTCAGGCT TCGCAGGTCT 
GAGATGAAGT TGTGGGGTCC AGACGCAGAT GGTGATGTGA AGGAGAAAAA GCGCAGCTCG 
TTGTTTTCAC CCCGTAAAAG CAGGAAGAAT GGAAATGCAG CAGCTGAGAG CGGGCGAGAG 
ACAGGGAAGC ACAAGTCCCT CTGGAAGACT GTTTTTTCAG TGTATAAGAA GGATAAAAAG 
AGGAAGGAGG TGGCAGTGGT GGCGGAAACA CTTCCTGCTG CTAATAATGG GAGCAAACGG 
AAAGTGTCGG GAATTAACAG AACCACAGAC CTGTGTTTCC GGAAGAATCC AAGTTTCTCT 
GAAGATACGG ATATGTCCTG TCATGCTCTT CTTGAAAGAT GTCCTCTCCG AGCTCAGAGA 
GCAGGAACAG AGGAGGAACT CAATGCCAGG CTGACACGTC GAGTGCAGAG AGCAGCGCGC 
AGACAGGCCA AGCAGGAGGA GCTGCGGAGG CTGCATAGAG CTCAGATAAT CCAGCGTCAG 
TTGGAGCAGG TGGAAGTGAA GCAGAGGCAG CTGGAGGAGA AAGGTGTGGC TGTGGAGAAA 
GCACTCAGAG GAGAAGCAGA CTTCTGGGAA GACTCTAGTA CCTCTGTCCT CTTGGATGTA 
CATCTGTGTG GAATGGGAAA GAAAGACGAT CCAAGCTTAA TGCATCAGTG GTTCAAACTG 
GTGCAGGAGA AGAATGCTCT CGTGCGTTAT GAATCTGAGC TCATGATATT CGCTCGTGAA 
CTGGAGCTGG AGGACAGACA GAGTCGCCTG CAGCAGGAGC TGAGAGAGCG CATGGCGGTG 
GACGATCATC TAAAAGGAGA AGAGGAGCTG GCGGAGGAGA GGCGTATATT GAGCGAGATG 
CTGGATGTGG TGGAGCAGAG GGATGCACTG GTGGCCCTCC TGGAGGAGCA GAGAGTTCGA 
GAAAAAGAGG AGGACAGTGA CCTGGAGGCT GTGATGCTGT CTAAGGGCTT CAGTCTGCAT 
TGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( NM_001353875.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001340804.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), mRNA.

Price & Availability↑

CloneID	ODa44979
Clone ID Related Accession (Same CDS sequence)	NM_001353876.1 , NM_001353875.1
Accession Version	NM_001353875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4989bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-19
Organism	Danio rerio(zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX649444.12 and BX901962.8. On May 20, 2019 this sequence version replaced NM_001353876.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01026062.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
GGTTCTCAGA AGGATCTTCC AGTAAATGTC GGCAGTAGTG ATGTGTGTTA TTTCTGTGGC 
CGTCGCGTTT ATGTCATGGA GAGGCTGAGC GCAGAGGGAA AGTTCTTCCA CAGGAGCTGC 
TTCCAGTGCG ACCACTGCAG CTCCACGATA CGCCTCTCCA ACTACACTTA TGACCAGCTA 
CATGGCAAGT TTTACTGTAA GCATCACTTC AGCTTCAGAC TGGCAAGTGT GGCTCAGAGA 
AAGAGACCGG CCCCACCGGT GGCCCCTCGG CCTGCTCAGG CTTCCCTGGC TGCATCCTCG 
GCCTCTACCT CTTTGTCCTC TTTGGGCTCA GTGGGCACAG CCACACCAGA CAGCTGGTCC 
TCCAGCACCC ACACAGACAT GGCATCCAGC TTGGCCAAGA GACTGTGCGG CACACCAGAA 
CGCATTGAGC TTGAGAATTA CAAACCCTCC CCACAGAAAC AAGACAGCCC CCTGCAGGAG 
GTACCAGAGG AAACCCTCGC ACAGCACAAT CTCAGCGCTA GCCTGCAGGA GAAGAATGCT 
GAAGAGCAGT CCAGTAGCTC TGAGTCAGAT CTAGAGGAGG AGGAGCTTGT CTGGAAGAAG 
GGGGAGGAGC TACATGCCAG GACCAATGGA GAGAGGAAGC TTGACCTAGA GGAGGAGCTG 
AAGGAAGAAG AGGGAGGGGA GAAGCTGGAA AAACAAGAGG GGGAGGAAGA AGGAGAAGTT 
TCAGAAGAAG AGCAAGACGA GGGAGATTCT AGTGATGAGG GTGAGTACTG CCCTTGGGAG 
AGGGAACGAC TCTCAGGGGT GTGGCTTGAG GAAGAGGAGG CGGGGTTTGT TAAAGAATCT 
TATGAAGGAT GCAGCGACGA CCCTGATATA GACGTCAGCT CACTCAGTGA GTCCAAGCTA 
TGTGACCAAC GGGAACAGGA GGAGTCTGTT CCTTTCCACC AATCCCCTGC CTCTACAGAA 
TCTCCAGTCT CAATGAAACC TTCAGAATCA GACCTCACAC CTGATCCGTC AACAACCCCC 
GAGAGCTCGC CTGCCAAGAG GTCAGAGGTG GTGGAGGAGT TCTGGATGAA GAGTGCAGAG 
ATCCGAAAGA GTTTAGGCCT GACTCCGCTT TCTAGAGAAT TGCAGCCAAA ACATATAGCA 
GCTTCAACCC AGACATCTAA TGTTAAAGAA AGCTTTTACA CCTCAGTCAC ATATAACACC 
GCTGCTTTGG ATTCTCCAAA CCAAAGTGCT AGAATCTGTG ACTCCAGCAC ACAAACTCAT 
TCTGTGACAG ATCTTCAAGA AACCTCCCCT CTTGGACCTA CAGATGGCGA TGCTGGCGTC 
GATTTAGGAC GTTGTTCTGT TGTTCACAGA CTCAGCATCA CTGTAGAAGG TTGTGTGATG 
GCAGATAACC AGGGTTTGGA TTCTACCTCG TTTGCTACTG AAAGTGTTTT AAACCCAGAC 
GCAGGTCTTC CCACCCCTCC TTATAGTCCG TCTCACTCCC CTCTTGTTGG TAAACAGTGT 
CGTGCCCTTC ACCATTCTGA GCCAATTCTA GACAGAGAGG TTATGGCTTT CTCATCTACC 
TGCACTGCTT CTGTACCACA GCGGAGCAGT TTTAAATCAG ACCTCGAGCA AGCTCAAAGT 
CTTCCACCAG ATGAAATCGA GATCCTGTGC GGAGACGAGG CGGAAAAACT CCCGGAAAGA 
TCTTGCAGGA TGGAGAGCGG AGAGGTGGAT AACCGTAGAG CGGAGCACAG CAGGACGCTT 
CCTGACCGGG TGGTCGCACC GCTGCTGGCA GGGGGACCAG AGGTCAGGCT TCGCAGGTCT 
GAGATGAAGT TGTGGGGTCC AGACGCAGAT GGTGATGTGA AGGAGAAAAA GCGCAGCTCG 
TTGTTTTCAC CCCGTAAAAG CAGGAAGAAT GGAAATGCAG CAGCTGAGAG CGGGCGAGAG 
ACAGGGAAGC ACAAGTCCCT CTGGAAGACT GTTTTTTCAG TGTATAAGAA GGATAAAAAG 
AGGAAGGAGG TGGCAGTGGT GGCGGAAACA CTTCCTGCTG CTAATAATGG GAGCAAACGG 
AAAGTGTCGG GAATTAACAG AACCACAGAC CTGTGTTTCC GGAAGAATCC AAGTTTCTCT 
GAAGATACGG ATATGTCCTG TCATGCTCTT CTTGAAAGAT GTCCTCTCCG AGCTCAGAGA 
GCAGGAACAG AGGAGGAACT CAATGCCAGG CTGACACGTC GAGTGCAGAG AGCAGCGCGC 
AGACAGGCCA AGCAGGAGGA GCTGCGGAGG CTGCATAGAG CTCAGATAAT CCAGCGTCAG 
TTGGAGCAGG TGGAAGTGAA GCAGAGGCAG CTGGAGGAGA AAGGTGTGGC TGTGGAGAAA 
GCACTCAGAG GAGAAGCAGA CTTCTGGGAA GACTCTAGTA CCTCTGTCCT CTTGGATGTA 
CATCTGTGTG GAATGGGAAA GAAAGACGAT CCAAGCTTAA TGCATCAGTG GTTCAAACTG 
GTGCAGGAGA AGAATGCTCT CGTGCGTTAT GAATCTGAGC TCATGATATT CGCTCGTGAA 
CTGGAGCTGG AGGACAGACA GAGTCGCCTG CAGCAGGAGC TGAGAGAGCG CATGGCGGTG 
GACGATCATC TAAAAGGAGA AGAGGAGCTG GCGGAGGAGA GGCGTATATT GAGCGAGATG 
CTGGATGTGG TGGAGCAGAG GGATGCACTG GTGGCCCTCC TGGAGGAGCA GAGAGTTCGA 
GAAAAAGAGG AGGACAGTGA CCTGGAGGCT GTGATGCTGT CTAAGGGCTT CAGTCTGCAT 
TGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340804.1
CDS	232..5220
Translation	MWDGQSEMCQAHVLFDSFVQAATCKETLRAFQDLCEELNLNPGGQPQFYHTLRSRLHYWKAKALWAKLDKRACQKEYMRGHACTSTTCLIIGAGPCGLRTAIELGFLGARVVLVEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRKVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTAIRRQGSQKDLPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSSESDLEEEELVWKKGEELHARTNGERKLDLEEELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDEGEYCPWERERLSGVWLEEEEAGFVKESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNGSKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEADFWEDSSTSVLLDVHLCGMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	NM_001353875.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353875.1

ORF Insert Sequence:

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
GGTTCTCAGA AGGATCTTCC AGTAAATGTC GGCAGTAGTG ATGTGTGTTA TTTCTGTGGC 
CGTCGCGTTT ATGTCATGGA GAGGCTGAGC GCAGAGGGAA AGTTCTTCCA CAGGAGCTGC 
TTCCAGTGCG ACCACTGCAG CTCCACGATA CGCCTCTCCA ACTACACTTA TGACCAGCTA 
CATGGCAAGT TTTACTGTAA GCATCACTTC AGCTTCAGAC TGGCAAGTGT GGCTCAGAGA 
AAGAGACCGG CCCCACCGGT GGCCCCTCGG CCTGCTCAGG CTTCCCTGGC TGCATCCTCG 
GCCTCTACCT CTTTGTCCTC TTTGGGCTCA GTGGGCACAG CCACACCAGA CAGCTGGTCC 
TCCAGCACCC ACACAGACAT GGCATCCAGC TTGGCCAAGA GACTGTGCGG CACACCAGAA 
CGCATTGAGC TTGAGAATTA CAAACCCTCC CCACAGAAAC AAGACAGCCC CCTGCAGGAG 
GTACCAGAGG AAACCCTCGC ACAGCACAAT CTCAGCGCTA GCCTGCAGGA GAAGAATGCT 
GAAGAGCAGT CCAGTAGCTC TGAGTCAGAT CTAGAGGAGG AGGAGCTTGT CTGGAAGAAG 
GGGGAGGAGC TACATGCCAG GACCAATGGA GAGAGGAAGC TTGACCTAGA GGAGGAGCTG 
AAGGAAGAAG AGGGAGGGGA GAAGCTGGAA AAACAAGAGG GGGAGGAAGA AGGAGAAGTT 
TCAGAAGAAG AGCAAGACGA GGGAGATTCT AGTGATGAGG GTGAGTACTG CCCTTGGGAG 
AGGGAACGAC TCTCAGGGGT GTGGCTTGAG GAAGAGGAGG CGGGGTTTGT TAAAGAATCT 
TATGAAGGAT GCAGCGACGA CCCTGATATA GACGTCAGCT CACTCAGTGA GTCCAAGCTA 
TGTGACCAAC GGGAACAGGA GGAGTCTGTT CCTTTCCACC AATCCCCTGC CTCTACAGAA 
TCTCCAGTCT CAATGAAACC TTCAGAATCA GACCTCACAC CTGATCCGTC AACAACCCCC 
GAGAGCTCGC CTGCCAAGAG GTCAGAGGTG GTGGAGGAGT TCTGGATGAA GAGTGCAGAG 
ATCCGAAAGA GTTTAGGCCT GACTCCGCTT TCTAGAGAAT TGCAGCCAAA ACATATAGCA 
GCTTCAACCC AGACATCTAA TGTTAAAGAA AGCTTTTACA CCTCAGTCAC ATATAACACC 
GCTGCTTTGG ATTCTCCAAA CCAAAGTGCT AGAATCTGTG ACTCCAGCAC ACAAACTCAT 
TCTGTGACAG ATCTTCAAGA AACCTCCCCT CTTGGACCTA CAGATGGCGA TGCTGGCGTC 
GATTTAGGAC GTTGTTCTGT TGTTCACAGA CTCAGCATCA CTGTAGAAGG TTGTGTGATG 
GCAGATAACC AGGGTTTGGA TTCTACCTCG TTTGCTACTG AAAGTGTTTT AAACCCAGAC 
GCAGGTCTTC CCACCCCTCC TTATAGTCCG TCTCACTCCC CTCTTGTTGG TAAACAGTGT 
CGTGCCCTTC ACCATTCTGA GCCAATTCTA GACAGAGAGG TTATGGCTTT CTCATCTACC 
TGCACTGCTT CTGTACCACA GCGGAGCAGT TTTAAATCAG ACCTCGAGCA AGCTCAAAGT 
CTTCCACCAG ATGAAATCGA GATCCTGTGC GGAGACGAGG CGGAAAAACT CCCGGAAAGA 
TCTTGCAGGA TGGAGAGCGG AGAGGTGGAT AACCGTAGAG CGGAGCACAG CAGGACGCTT 
CCTGACCGGG TGGTCGCACC GCTGCTGGCA GGGGGACCAG AGGTCAGGCT TCGCAGGTCT 
GAGATGAAGT TGTGGGGTCC AGACGCAGAT GGTGATGTGA AGGAGAAAAA GCGCAGCTCG 
TTGTTTTCAC CCCGTAAAAG CAGGAAGAAT GGAAATGCAG CAGCTGAGAG CGGGCGAGAG 
ACAGGGAAGC ACAAGTCCCT CTGGAAGACT GTTTTTTCAG TGTATAAGAA GGATAAAAAG 
AGGAAGGAGG TGGCAGTGGT GGCGGAAACA CTTCCTGCTG CTAATAATGG GAGCAAACGG 
AAAGTGTCGG GAATTAACAG AACCACAGAC CTGTGTTTCC GGAAGAATCC AAGTTTCTCT 
GAAGATACGG ATATGTCCTG TCATGCTCTT CTTGAAAGAT GTCCTCTCCG AGCTCAGAGA 
GCAGGAACAG AGGAGGAACT CAATGCCAGG CTGACACGTC GAGTGCAGAG AGCAGCGCGC 
AGACAGGCCA AGCAGGAGGA GCTGCGGAGG CTGCATAGAG CTCAGATAAT CCAGCGTCAG 
TTGGAGCAGG TGGAAGTGAA GCAGAGGCAG CTGGAGGAGA AAGGTGTGGC TGTGGAGAAA 
GCACTCAGAG GAGAAGCAGA CTTCTGGGAA GACTCTAGTA CCTCTGTCCT CTTGGATGTA 
CATCTGTGTG GAATGGGAAA GAAAGACGAT CCAAGCTTAA TGCATCAGTG GTTCAAACTG 
GTGCAGGAGA AGAATGCTCT CGTGCGTTAT GAATCTGAGC TCATGATATT CGCTCGTGAA 
CTGGAGCTGG AGGACAGACA GAGTCGCCTG CAGCAGGAGC TGAGAGAGCG CATGGCGGTG 
GACGATCATC TAAAAGGAGA AGAGGAGCTG GCGGAGGAGA GGCGTATATT GAGCGAGATG 
CTGGATGTGG TGGAGCAGAG GGATGCACTG GTGGCCCTCC TGGAGGAGCA GAGAGTTCGA 
GAAAAAGAGG AGGACAGTGA CCTGGAGGCT GTGATGCTGT CTAAGGGCTT CAGTCTGCAT 
TGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( XM_021475215.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330890.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X2, mRNA.

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CloneID	ODa142123
Clone ID Related Accession (Same CDS sequence)	XM_021475215.1
Accession Version	XM_021475215.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGCTCTG AGTCAGATCT AGAGGAGGAG GAGCTTGTCT GGAAGAAGGG GGAGGAGCTA 
CATGCCAGGA CCAATGGAGA GAGGAAGCTT GACCTAGAGG AGGAGCTGAA GGAAGAAGAG 
GGAGGGGAGA AGCTGGAAAA ACAAGAGGGG GAGGAAGAAG GAGAAGTTTC AGAAGAAGAG 
CAAGACGAGG GAGATTCTAG TGATGAGGGT GAGTACTGCC CTTGGGAGAG GGAACGACTC 
TCAGGGGTGT GGCTTGAGGA AGAGGAGGCG GGGTTTGTTA AAGAATCTTA TGAAGGATGC 
AGCGACGACC CTGATATAGA CGTCAGCTCA CTCAGTGAGT CCAAGCTATG TGACCAACGG 
GAACAGGAGG AGTCTGTTCC TTTCCACCAA TCCCCTGCCT CTACAGAATC TCCAGTCTCA 
ATGAAACCTT CAGAATCAGA CCTCACACCT GATCCGTCAA CAACCCCCGA GAGCTCGCCT 
GCCAAGAGGT CAGAGGTGGT GGAGGAGTTC TGGATGAAGA GTGCAGAGAT CCGAAAGAGT 
TTAGGCCTGA CTCCGCTTTC TAGAGAATTG CAGCCAAAAC ATATAGCAGC TTCAACCCAG 
ACATCTAATG TTAAAGAAAG CTTTTACACC TCAGTCACAT ATAACACCGC TGCTTTGGAT 
TCTCCAAACC AAAGTGCTAG AATCTGTGAC TCCAGCACAC AAACTCATTC TGTGACAGAT 
CTTCAAGAAA CCTCCCCTCT TGGACCTACA GATGGCGATG CTGGCGTCGA TTTAGGACGT 
TGTTCTGTTG TTCACAGACT CAGCATCACT GTAGAAGGTT GTGTGATGGC AGATAACCAG 
GGTTTGGATT CTACCTCGTT TGCTACTGAA AGTGTTTTAA ACCCAGACGC AGGTCTTCCC 
ACCCCTCCTT ATAGTCCGTC TCACTCCCCT CTTGTTGGTA AACAGTGTCG TGCCCTTCAC 
CATTCTGAGC CAATTCTAGA CAGAGAGGTT ATGGCTTTCT CATCTACCTG CACTGCTTCT 
GTACCACAGC GGAGCAGTTT TAAATCAGAC CTCGAGCAAG CTCAAAGTCT TCCACCAGAT 
GAAATCGAGA TCCTGTGCGG AGACGAGGCG GAAAAACTCC CGGAAAGATC TTGCAGGATG 
GAGAGCGGAG AGGTGGATAA CCGTAGAGCG GAGCACAGCA GGACGCTTCC TGACCGGGTG 
GTCGCACCGC TGCTGGCAGG GGGACCAGAG GTCAGGCTTC GCAGGTCTGA GATGAAGTTG 
TGGGGTCCAG ACGCAGATGG TGATGTGAAG GAGAAAAAGC GCAGCTCGTT GTTTTCACCC 
CGTAAAAGCA GGAAGAATGG AAATGCAGCA GCTGAGAGCG GGCGAGAGAC AGGGAAGCAC 
AAGTCCCTCT GGAAGACTGT TTTTTCAGTG TATAAGAAGG ATAAAAAGAG GAAGGAGGTG 
GCAGTGGTGG CGGAAACACT TCCTGCTGCT AATAATGGGA GCAAACGGAA AGTGTCGGGA 
ATTAACAGAA CCACAGACCT GTGTTTCCGG AAGAATCCAA GTTTCTCTGA AGATACGGAT 
ATGTCCTGTC ATGCTCTTCT TGAAAGATGT CCTCTCCGAG CTCAGAGAGC AGGAACAGAG 
GAGGAACTCA ATGCCAGGCT GACACGTCGA GTGCAGAGAG CAGCGCGCAG ACAGGCCAAG 
CAGGAGGAGC TGCGGAGGCT GCATAGAGCT CAGATAATCC AGCGTCAGTT GGAGCAGGTG 
GAAGTGAAGC AGAGGCAGCT GGAGGAGAAA GGTGTGGCTG TGGAGAAAGC ACTCAGAGGA 
GAAGCAGTTG AGCCTTTTGA CGGTTCTCCC CGTAGAAGGC CTGTATATCT CTGTCCTTGC 
TGTTCCTCTG AAGCCAAAGC TAAAGGAACC GAACATGATC ATTCGGTTTA TGTCTGTACA 
GTCTTTTTCC TGTACGTAGT GCAGGAGCCT CCACTTCACC AACTGAGAAT GGGAAAGAAA 
GACGATCCAA GCTTAATGCA TCAGTGGTTC AAACTGGTGC AGGAGAAGAA TGCTCTCGTG 
CGTTATGAAT CTGAGCTCAT GATATTCGCT CGTGAACTGG AGCTGGAGGA CAGACAGAGT 
CGCCTGCAGC AGGAGCTGAG AGAGCGCATG GCGGTGGACG ATCATCTAAA AGGAGAAGAG 
GAGCTGGCGG AGGAGAGGCG TATATTGAGC GAGATGCTGG ATGTGGTGGA GCAGAGGGAT 
GCACTGGTGG CCCTCCTGGA GGAGCAGAGA GTTCGAGAAA AAGAGGAGGA CAGTGACCTG 
GAGGCTGTGA TGCTGTCTAA GGGCTTCAGT CTGCATTGGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330890.1
CDS	227..5431
Translation	MWDGQSEMCQAHVLFDSFVQAATCKETLRAFQDLCEELNLNPGGQPQFYHTLRSRLHYWKAKALWAKLDKRACQKEYMRGHACTSTTCLIIGAGPCGLRTAIELGFLGARVVLVEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRKVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTAIRRQNYIHMYTGGVSSLALQIANQIQSQQAQAPKLLHRRESGSQKDLPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSESDLEEEELVWKKGEELHARTNGERKLDLEEELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDEGEYCPWERERLSGVWLEEEEAGFVKESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNGSKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEAVEPFDGSPRRRPVYLCPCCSSEAKAKGTEHDHSVYVCTVFFLYVVQEPPLHQLRMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	XM_021475215.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475215.1

ORF Insert Sequence:

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGCTCTG AGTCAGATCT AGAGGAGGAG GAGCTTGTCT GGAAGAAGGG GGAGGAGCTA 
CATGCCAGGA CCAATGGAGA GAGGAAGCTT GACCTAGAGG AGGAGCTGAA GGAAGAAGAG 
GGAGGGGAGA AGCTGGAAAA ACAAGAGGGG GAGGAAGAAG GAGAAGTTTC AGAAGAAGAG 
CAAGACGAGG GAGATTCTAG TGATGAGGGT GAGTACTGCC CTTGGGAGAG GGAACGACTC 
TCAGGGGTGT GGCTTGAGGA AGAGGAGGCG GGGTTTGTTA AAGAATCTTA TGAAGGATGC 
AGCGACGACC CTGATATAGA CGTCAGCTCA CTCAGTGAGT CCAAGCTATG TGACCAACGG 
GAACAGGAGG AGTCTGTTCC TTTCCACCAA TCCCCTGCCT CTACAGAATC TCCAGTCTCA 
ATGAAACCTT CAGAATCAGA CCTCACACCT GATCCGTCAA CAACCCCCGA GAGCTCGCCT 
GCCAAGAGGT CAGAGGTGGT GGAGGAGTTC TGGATGAAGA GTGCAGAGAT CCGAAAGAGT 
TTAGGCCTGA CTCCGCTTTC TAGAGAATTG CAGCCAAAAC ATATAGCAGC TTCAACCCAG 
ACATCTAATG TTAAAGAAAG CTTTTACACC TCAGTCACAT ATAACACCGC TGCTTTGGAT 
TCTCCAAACC AAAGTGCTAG AATCTGTGAC TCCAGCACAC AAACTCATTC TGTGACAGAT 
CTTCAAGAAA CCTCCCCTCT TGGACCTACA GATGGCGATG CTGGCGTCGA TTTAGGACGT 
TGTTCTGTTG TTCACAGACT CAGCATCACT GTAGAAGGTT GTGTGATGGC AGATAACCAG 
GGTTTGGATT CTACCTCGTT TGCTACTGAA AGTGTTTTAA ACCCAGACGC AGGTCTTCCC 
ACCCCTCCTT ATAGTCCGTC TCACTCCCCT CTTGTTGGTA AACAGTGTCG TGCCCTTCAC 
CATTCTGAGC CAATTCTAGA CAGAGAGGTT ATGGCTTTCT CATCTACCTG CACTGCTTCT 
GTACCACAGC GGAGCAGTTT TAAATCAGAC CTCGAGCAAG CTCAAAGTCT TCCACCAGAT 
GAAATCGAGA TCCTGTGCGG AGACGAGGCG GAAAAACTCC CGGAAAGATC TTGCAGGATG 
GAGAGCGGAG AGGTGGATAA CCGTAGAGCG GAGCACAGCA GGACGCTTCC TGACCGGGTG 
GTCGCACCGC TGCTGGCAGG GGGACCAGAG GTCAGGCTTC GCAGGTCTGA GATGAAGTTG 
TGGGGTCCAG ACGCAGATGG TGATGTGAAG GAGAAAAAGC GCAGCTCGTT GTTTTCACCC 
CGTAAAAGCA GGAAGAATGG AAATGCAGCA GCTGAGAGCG GGCGAGAGAC AGGGAAGCAC 
AAGTCCCTCT GGAAGACTGT TTTTTCAGTG TATAAGAAGG ATAAAAAGAG GAAGGAGGTG 
GCAGTGGTGG CGGAAACACT TCCTGCTGCT AATAATGGGA GCAAACGGAA AGTGTCGGGA 
ATTAACAGAA CCACAGACCT GTGTTTCCGG AAGAATCCAA GTTTCTCTGA AGATACGGAT 
ATGTCCTGTC ATGCTCTTCT TGAAAGATGT CCTCTCCGAG CTCAGAGAGC AGGAACAGAG 
GAGGAACTCA ATGCCAGGCT GACACGTCGA GTGCAGAGAG CAGCGCGCAG ACAGGCCAAG 
CAGGAGGAGC TGCGGAGGCT GCATAGAGCT CAGATAATCC AGCGTCAGTT GGAGCAGGTG 
GAAGTGAAGC AGAGGCAGCT GGAGGAGAAA GGTGTGGCTG TGGAGAAAGC ACTCAGAGGA 
GAAGCAGTTG AGCCTTTTGA CGGTTCTCCC CGTAGAAGGC CTGTATATCT CTGTCCTTGC 
TGTTCCTCTG AAGCCAAAGC TAAAGGAACC GAACATGATC ATTCGGTTTA TGTCTGTACA 
GTCTTTTTCC TGTACGTAGT GCAGGAGCCT CCACTTCACC AACTGAGAAT GGGAAAGAAA 
GACGATCCAA GCTTAATGCA TCAGTGGTTC AAACTGGTGC AGGAGAAGAA TGCTCTCGTG 
CGTTATGAAT CTGAGCTCAT GATATTCGCT CGTGAACTGG AGCTGGAGGA CAGACAGAGT 
CGCCTGCAGC AGGAGCTGAG AGAGCGCATG GCGGTGGACG ATCATCTAAA AGGAGAAGAG 
GAGCTGGCGG AGGAGAGGCG TATATTGAGC GAGATGCTGG ATGTGGTGGA GCAGAGGGAT 
GCACTGGTGG CCCTCCTGGA GGAGCAGAGA GTTCGAGAAA AAGAGGAGGA CAGTGACCTG 
GAGGCTGTGA TGCTGTCTAA GGGCTTCAGT CTGCATTGGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( XM_021475216.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330891.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X3, mRNA.

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CloneID	ODa142124
Clone ID Related Accession (Same CDS sequence)	XM_021475216.1
Accession Version	XM_021475216.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGAGGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAA TCTTATGAAG GATGCAGCGA CGACCCTGAT 
ATAGACGTCA GCTCACTCAG TGAGTCCAAG CTATGTGACC AACGGGAACA GGAGGAGTCT 
GTTCCTTTCC ACCAATCCCC TGCCTCTACA GAATCTCCAG TCTCAATGAA ACCTTCAGAA 
TCAGACCTCA CACCTGATCC GTCAACAACC CCCGAGAGCT CGCCTGCCAA GAGGTCAGAG 
GTGGTGGAGG AGTTCTGGAT GAAGAGTGCA GAGATCCGAA AGAGTTTAGG CCTGACTCCG 
CTTTCTAGAG AATTGCAGCC AAAACATATA GCAGCTTCAA CCCAGACATC TAATGTTAAA 
GAAAGCTTTT ACACCTCAGT CACATATAAC ACCGCTGCTT TGGATTCTCC AAACCAAAGT 
GCTAGAATCT GTGACTCCAG CACACAAACT CATTCTGTGA CAGATCTTCA AGAAACCTCC 
CCTCTTGGAC CTACAGATGG CGATGCTGGC GTCGATTTAG GACGTTGTTC TGTTGTTCAC 
AGACTCAGCA TCACTGTAGA AGGTTGTGTG ATGGCAGATA ACCAGGGTTT GGATTCTACC 
TCGTTTGCTA CTGAAAGTGT TTTAAACCCA GACGCAGGTC TTCCCACCCC TCCTTATAGT 
CCGTCTCACT CCCCTCTTGT TGGTAAACAG TGTCGTGCCC TTCACCATTC TGAGCCAATT 
CTAGACAGAG AGGTTATGGC TTTCTCATCT ACCTGCACTG CTTCTGTACC ACAGCGGAGC 
AGTTTTAAAT CAGACCTCGA GCAAGCTCAA AGTCTTCCAC CAGATGAAAT CGAGATCCTG 
TGCGGAGACG AGGCGGAAAA ACTCCCGGAA AGATCTTGCA GGATGGAGAG CGGAGAGGTG 
GATAACCGTA GAGCGGAGCA CAGCAGGACG CTTCCTGACC GGGTGGTCGC ACCGCTGCTG 
GCAGGGGGAC CAGAGGTCAG GCTTCGCAGG TCTGAGATGA AGTTGTGGGG TCCAGACGCA 
GATGGTGATG TGAAGGAGAA AAAGCGCAGC TCGTTGTTTT CACCCCGTAA AAGCAGGAAG 
AATGGAAATG CAGCAGCTGA GAGCGGGCGA GAGACAGGGA AGCACAAGTC CCTCTGGAAG 
ACTGTTTTTT CAGTGTATAA GAAGGATAAA AAGAGGAAGG AGGTGGCAGT GGTGGCGGAA 
ACACTTCCTG CTGCTAATAA TGGGAGCAAA CGGAAAGTGT CGGGAATTAA CAGAACCACA 
GACCTGTGTT TCCGGAAGAA TCCAAGTTTC TCTGAAGATA CGGATATGTC CTGTCATGCT 
CTTCTTGAAA GATGTCCTCT CCGAGCTCAG AGAGCAGGAA CAGAGGAGGA ACTCAATGCC 
AGGCTGACAC GTCGAGTGCA GAGAGCAGCG CGCAGACAGG CCAAGCAGGA GGAGCTGCGG 
AGGCTGCATA GAGCTCAGAT AATCCAGCGT CAGTTGGAGC AGGTGGAAGT GAAGCAGAGG 
CAGCTGGAGG AGAAAGGTGT GGCTGTGGAG AAAGCACTCA GAGGAGAAGC AGTTGAGCCT 
TTTGACGGTT CTCCCCGTAG AAGGCCTGTA TATCTCTGTC CTTGCTGTTC CTCTGAAGCC 
AAAGCTAAAG GAACCGAACA TGATCATTCG GTTTATGTCT GTACAGTCTT TTTCCTGTAC 
GTAGTGCAGG AGCCTCCACT TCACCAACTG AGAATGGGAA AGAAAGACGA TCCAAGCTTA 
ATGCATCAGT GGTTCAAACT GGTGCAGGAG AAGAATGCTC TCGTGCGTTA TGAATCTGAG 
CTCATGATAT TCGCTCGTGA ACTGGAGCTG GAGGACAGAC AGAGTCGCCT GCAGCAGGAG 
CTGAGAGAGC GCATGGCGGT GGACGATCAT CTAAAAGGAG AAGAGGAGCT GGCGGAGGAG 
AGGCGTATAT TGAGCGAGAT GCTGGATGTG GTGGAGCAGA GGGATGCACT GGTGGCCCTC 
CTGGAGGAGC AGAGAGTTCG AGAAAAAGAG GAGGACAGTG ACCTGGAGGC TGTGATGCTG 
TCTAAGGGCT TCAGTCTGCA TTGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330891.1
CDS	227..5356
Translation	MWDGQSEMCQAHVLFDSFVQAATCKETLRAFQDLCEELNLNPGGQPQFYHTLRSRLHYWKAKALWAKLDKRACQKEYMRGHACTSTTCLIIGAGPCGLRTAIELGFLGARVVLVEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRKVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTAIRRQNYIHMYTGGVSSLALQIANQIQSQQAQAPKLLHRRESGSQKDLPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSSESDLEEEELVWKKGEELHARTNGERKLDLEEELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNGSKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEAVEPFDGSPRRRPVYLCPCCSSEAKAKGTEHDHSVYVCTVFFLYVVQEPPLHQLRMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	XM_021475216.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475216.1

ORF Insert Sequence:

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGAGGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAA TCTTATGAAG GATGCAGCGA CGACCCTGAT 
ATAGACGTCA GCTCACTCAG TGAGTCCAAG CTATGTGACC AACGGGAACA GGAGGAGTCT 
GTTCCTTTCC ACCAATCCCC TGCCTCTACA GAATCTCCAG TCTCAATGAA ACCTTCAGAA 
TCAGACCTCA CACCTGATCC GTCAACAACC CCCGAGAGCT CGCCTGCCAA GAGGTCAGAG 
GTGGTGGAGG AGTTCTGGAT GAAGAGTGCA GAGATCCGAA AGAGTTTAGG CCTGACTCCG 
CTTTCTAGAG AATTGCAGCC AAAACATATA GCAGCTTCAA CCCAGACATC TAATGTTAAA 
GAAAGCTTTT ACACCTCAGT CACATATAAC ACCGCTGCTT TGGATTCTCC AAACCAAAGT 
GCTAGAATCT GTGACTCCAG CACACAAACT CATTCTGTGA CAGATCTTCA AGAAACCTCC 
CCTCTTGGAC CTACAGATGG CGATGCTGGC GTCGATTTAG GACGTTGTTC TGTTGTTCAC 
AGACTCAGCA TCACTGTAGA AGGTTGTGTG ATGGCAGATA ACCAGGGTTT GGATTCTACC 
TCGTTTGCTA CTGAAAGTGT TTTAAACCCA GACGCAGGTC TTCCCACCCC TCCTTATAGT 
CCGTCTCACT CCCCTCTTGT TGGTAAACAG TGTCGTGCCC TTCACCATTC TGAGCCAATT 
CTAGACAGAG AGGTTATGGC TTTCTCATCT ACCTGCACTG CTTCTGTACC ACAGCGGAGC 
AGTTTTAAAT CAGACCTCGA GCAAGCTCAA AGTCTTCCAC CAGATGAAAT CGAGATCCTG 
TGCGGAGACG AGGCGGAAAA ACTCCCGGAA AGATCTTGCA GGATGGAGAG CGGAGAGGTG 
GATAACCGTA GAGCGGAGCA CAGCAGGACG CTTCCTGACC GGGTGGTCGC ACCGCTGCTG 
GCAGGGGGAC CAGAGGTCAG GCTTCGCAGG TCTGAGATGA AGTTGTGGGG TCCAGACGCA 
GATGGTGATG TGAAGGAGAA AAAGCGCAGC TCGTTGTTTT CACCCCGTAA AAGCAGGAAG 
AATGGAAATG CAGCAGCTGA GAGCGGGCGA GAGACAGGGA AGCACAAGTC CCTCTGGAAG 
ACTGTTTTTT CAGTGTATAA GAAGGATAAA AAGAGGAAGG AGGTGGCAGT GGTGGCGGAA 
ACACTTCCTG CTGCTAATAA TGGGAGCAAA CGGAAAGTGT CGGGAATTAA CAGAACCACA 
GACCTGTGTT TCCGGAAGAA TCCAAGTTTC TCTGAAGATA CGGATATGTC CTGTCATGCT 
CTTCTTGAAA GATGTCCTCT CCGAGCTCAG AGAGCAGGAA CAGAGGAGGA ACTCAATGCC 
AGGCTGACAC GTCGAGTGCA GAGAGCAGCG CGCAGACAGG CCAAGCAGGA GGAGCTGCGG 
AGGCTGCATA GAGCTCAGAT AATCCAGCGT CAGTTGGAGC AGGTGGAAGT GAAGCAGAGG 
CAGCTGGAGG AGAAAGGTGT GGCTGTGGAG AAAGCACTCA GAGGAGAAGC AGTTGAGCCT 
TTTGACGGTT CTCCCCGTAG AAGGCCTGTA TATCTCTGTC CTTGCTGTTC CTCTGAAGCC 
AAAGCTAAAG GAACCGAACA TGATCATTCG GTTTATGTCT GTACAGTCTT TTTCCTGTAC 
GTAGTGCAGG AGCCTCCACT TCACCAACTG AGAATGGGAA AGAAAGACGA TCCAAGCTTA 
ATGCATCAGT GGTTCAAACT GGTGCAGGAG AAGAATGCTC TCGTGCGTTA TGAATCTGAG 
CTCATGATAT TCGCTCGTGA ACTGGAGCTG GAGGACAGAC AGAGTCGCCT GCAGCAGGAG 
CTGAGAGAGC GCATGGCGGT GGACGATCAT CTAAAAGGAG AAGAGGAGCT GGCGGAGGAG 
AGGCGTATAT TGAGCGAGAT GCTGGATGTG GTGGAGCAGA GGGATGCACT GGTGGCCCTC 
CTGGAGGAGC AGAGAGTTCG AGAAAAAGAG GAGGACAGTG ACCTGGAGGC TGTGATGCTG 
TCTAAGGGCT TCAGTCTGCA TTGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( XM_021475217.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330892.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X5, mRNA.

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CloneID	ODa142125
Clone ID Related Accession (Same CDS sequence)	XM_021475217.1
Accession Version	XM_021475217.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
GGTTCTCAGA AGGATCTTCC AGTAAATGTC GGCAGTAGTG ATGTGTGTTA TTTCTGTGGC 
CGTCGCGTTT ATGTCATGGA GAGGCTGAGC GCAGAGGGAA AGTTCTTCCA CAGGAGCTGC 
TTCCAGTGCG ACCACTGCAG CTCCACGATA CGCCTCTCCA ACTACACTTA TGACCAGCTA 
CATGGCAAGT TTTACTGTAA GCATCACTTC AGCTTCAGAC TGGCAAGTGT GGCTCAGAGA 
AAGAGACCGG CCCCACCGGT GGCCCCTCGG CCTGCTCAGG CTTCCCTGGC TGCATCCTCG 
GCCTCTACCT CTTTGTCCTC TTTGGGCTCA GTGGGCACAG CCACACCAGA CAGCTGGTCC 
TCCAGCACCC ACACAGACAT GGCATCCAGC TTGGCCAAGA GACTGTGCGG CACACCAGAA 
CGCATTGAGC TTGAGAATTA CAAACCCTCC CCACAGAAAC AAGACAGCCC CCTGCAGGAG 
GTACCAGAGG AAACCCTCGC ACAGCACAAT CTCAGCGCTA GCCTGCAGGA GAAGAATGCT 
GAAGAGCAGT CCAGTAGCTC TGAGTCAGAT CTAGAGGAGG AGGAGCTTGT CTGGAAGAAG 
GGGGAGGAGC TACATGCCAG GACCAATGGA GAGAGGAAGC TTGACCTAGA GGAGGAGCTG 
AAGGAAGAAG AGGGAGGGGA GAAGCTGGAA AAACAAGAGG GGGAGGAAGA AGGAGAAGTT 
TCAGAAGAAG AGCAAGACGA GGGAGATTCT AGTGATGAGG GTGAGTACTG CCCTTGGGAG 
AGGGAACGAC TCTCAGGGGT GTGGCTTGAG GAAGAGGAGG CGGGGTTTGT TAAAGAATCT 
TATGAAGGAT GCAGCGACGA CCCTGATATA GACGTCAGCT CACTCAGTGA GTCCAAGCTA 
TGTGACCAAC GGGAACAGGA GGAGTCTGTT CCTTTCCACC AATCCCCTGC CTCTACAGAA 
TCTCCAGTCT CAATGAAACC TTCAGAATCA GACCTCACAC CTGATCCGTC AACAACCCCC 
GAGAGCTCGC CTGCCAAGAG GTCAGAGGTG GTGGAGGAGT TCTGGATGAA GAGTGCAGAG 
ATCCGAAAGA GTTTAGGCCT GACTCCGCTT TCTAGAGAAT TGCAGCCAAA ACATATAGCA 
GCTTCAACCC AGACATCTAA TGTTAAAGAA AGCTTTTACA CCTCAGTCAC ATATAACACC 
GCTGCTTTGG ATTCTCCAAA CCAAAGTGCT AGAATCTGTG ACTCCAGCAC ACAAACTCAT 
TCTGTGACAG ATCTTCAAGA AACCTCCCCT CTTGGACCTA CAGATGGCGA TGCTGGCGTC 
GATTTAGGAC GTTGTTCTGT TGTTCACAGA CTCAGCATCA CTGTAGAAGG TTGTGTGATG 
GCAGATAACC AGGGTTTGGA TTCTACCTCG TTTGCTACTG AAAGTGTTTT AAACCCAGAC 
GCAGGTCTTC CCACCCCTCC TTATAGTCCG TCTCACTCCC CTCTTGTTGG TAAACAGTGT 
CGTGCCCTTC ACCATTCTGA GCCAATTCTA GACAGAGAGG TTATGGCTTT CTCATCTACC 
TGCACTGCTT CTGTACCACA GCGGAGCAGT TTTAAATCAG ACCTCGAGCA AGCTCAAAGT 
CTTCCACCAG ATGAAATCGA GATCCTGTGC GGAGACGAGG CGGAAAAACT CCCGGAAAGA 
TCTTGCAGGA TGGAGAGCGG AGAGGTGGAT AACCGTAGAG CGGAGCACAG CAGGACGCTT 
CCTGACCGGG TGGTCGCACC GCTGCTGGCA GGGGGACCAG AGGTCAGGCT TCGCAGGTCT 
GAGATGAAGT TGTGGGGTCC AGACGCAGAT GGTGATGTGA AGGAGAAAAA GCGCAGCTCG 
TTGTTTTCAC CCCGTAAAAG CAGGAAGAAT GGAAATGCAG CAGCTGAGAG CGGGCGAGAG 
ACAGGGAAGC ACAAGTCCCT CTGGAAGACT GTTTTTTCAG TGTATAAGAA GGATAAAAAG 
AGGAAGGAGG TGGCAGTGGT GGCGGAAACA CTTCCTGCTG CTAATAATGG GAGCAAACGG 
AAAGTGTCGG GAATTAACAG AACCACAGAC CTGTGTTTCC GGAAGAATCC AAGTTTCTCT 
GAAGATACGG ATATGTCCTG TCATGCTCTT CTTGAAAGAT GTCCTCTCCG AGCTCAGAGA 
GCAGGAACAG AGGAGGAACT CAATGCCAGG CTGACACGTC GAGTGCAGAG AGCAGCGCGC 
AGACAGGCCA AGCAGGAGGA GCTGCGGAGG CTGCATAGAG CTCAGATAAT CCAGCGTCAG 
TTGGAGCAGG TGGAAGTGAA GCAGAGGCAG CTGGAGGAGA AAGGTGTGGC TGTGGAGAAA 
GCACTCAGAG GAGAAGCAGT TGAGCCTTTT GACGGTTCTC CCCGTAGAAG GCCTGTATAT 
CTCTGTCCTT GCTGTTCCTC TGAAGCCAAA GCTAAAGGAA CCGAACATGA TCATTCGGTT 
TATGTCTGTA CAGTCTTTTT CCTGTACGTA GTGCAGGAGC CTCCACTTCA CCAACTGAGA 
ATGGGAAAGA AAGACGATCC AAGCTTAATG CATCAGTGGT TCAAACTGGT GCAGGAGAAG 
AATGCTCTCG TGCGTTATGA ATCTGAGCTC ATGATATTCG CTCGTGAACT GGAGCTGGAG 
GACAGACAGA GTCGCCTGCA GCAGGAGCTG AGAGAGCGCA TGGCGGTGGA CGATCATCTA 
AAAGGAGAAG AGGAGCTGGC GGAGGAGAGG CGTATATTGA GCGAGATGCT GGATGTGGTG 
GAGCAGAGGG ATGCACTGGT GGCCCTCCTG GAGGAGCAGA GAGTTCGAGA AAAAGAGGAG 
GACAGTGACC TGGAGGCTGT GATGCTGTCT AAGGGCTTCA GTCTGCATTG GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330892.1
CDS	227..5323
Translation	MWDGQSEMCQAHVLFDSFVQAATCKETLRAFQDLCEELNLNPGGQPQFYHTLRSRLHYWKAKALWAKLDKRACQKEYMRGHACTSTTCLIIGAGPCGLRTAIELGFLGARVVLVEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRKVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTAIRRQGSQKDLPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSSESDLEEEELVWKKGEELHARTNGERKLDLEEELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDEGEYCPWERERLSGVWLEEEEAGFVKESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNGSKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEAVEPFDGSPRRRPVYLCPCCSSEAKAKGTEHDHSVYVCTVFFLYVVQEPPLHQLRMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	XM_021475217.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475217.1

ORF Insert Sequence:

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
GGTTCTCAGA AGGATCTTCC AGTAAATGTC GGCAGTAGTG ATGTGTGTTA TTTCTGTGGC 
CGTCGCGTTT ATGTCATGGA GAGGCTGAGC GCAGAGGGAA AGTTCTTCCA CAGGAGCTGC 
TTCCAGTGCG ACCACTGCAG CTCCACGATA CGCCTCTCCA ACTACACTTA TGACCAGCTA 
CATGGCAAGT TTTACTGTAA GCATCACTTC AGCTTCAGAC TGGCAAGTGT GGCTCAGAGA 
AAGAGACCGG CCCCACCGGT GGCCCCTCGG CCTGCTCAGG CTTCCCTGGC TGCATCCTCG 
GCCTCTACCT CTTTGTCCTC TTTGGGCTCA GTGGGCACAG CCACACCAGA CAGCTGGTCC 
TCCAGCACCC ACACAGACAT GGCATCCAGC TTGGCCAAGA GACTGTGCGG CACACCAGAA 
CGCATTGAGC TTGAGAATTA CAAACCCTCC CCACAGAAAC AAGACAGCCC CCTGCAGGAG 
GTACCAGAGG AAACCCTCGC ACAGCACAAT CTCAGCGCTA GCCTGCAGGA GAAGAATGCT 
GAAGAGCAGT CCAGTAGCTC TGAGTCAGAT CTAGAGGAGG AGGAGCTTGT CTGGAAGAAG 
GGGGAGGAGC TACATGCCAG GACCAATGGA GAGAGGAAGC TTGACCTAGA GGAGGAGCTG 
AAGGAAGAAG AGGGAGGGGA GAAGCTGGAA AAACAAGAGG GGGAGGAAGA AGGAGAAGTT 
TCAGAAGAAG AGCAAGACGA GGGAGATTCT AGTGATGAGG GTGAGTACTG CCCTTGGGAG 
AGGGAACGAC TCTCAGGGGT GTGGCTTGAG GAAGAGGAGG CGGGGTTTGT TAAAGAATCT 
TATGAAGGAT GCAGCGACGA CCCTGATATA GACGTCAGCT CACTCAGTGA GTCCAAGCTA 
TGTGACCAAC GGGAACAGGA GGAGTCTGTT CCTTTCCACC AATCCCCTGC CTCTACAGAA 
TCTCCAGTCT CAATGAAACC TTCAGAATCA GACCTCACAC CTGATCCGTC AACAACCCCC 
GAGAGCTCGC CTGCCAAGAG GTCAGAGGTG GTGGAGGAGT TCTGGATGAA GAGTGCAGAG 
ATCCGAAAGA GTTTAGGCCT GACTCCGCTT TCTAGAGAAT TGCAGCCAAA ACATATAGCA 
GCTTCAACCC AGACATCTAA TGTTAAAGAA AGCTTTTACA CCTCAGTCAC ATATAACACC 
GCTGCTTTGG ATTCTCCAAA CCAAAGTGCT AGAATCTGTG ACTCCAGCAC ACAAACTCAT 
TCTGTGACAG ATCTTCAAGA AACCTCCCCT CTTGGACCTA CAGATGGCGA TGCTGGCGTC 
GATTTAGGAC GTTGTTCTGT TGTTCACAGA CTCAGCATCA CTGTAGAAGG TTGTGTGATG 
GCAGATAACC AGGGTTTGGA TTCTACCTCG TTTGCTACTG AAAGTGTTTT AAACCCAGAC 
GCAGGTCTTC CCACCCCTCC TTATAGTCCG TCTCACTCCC CTCTTGTTGG TAAACAGTGT 
CGTGCCCTTC ACCATTCTGA GCCAATTCTA GACAGAGAGG TTATGGCTTT CTCATCTACC 
TGCACTGCTT CTGTACCACA GCGGAGCAGT TTTAAATCAG ACCTCGAGCA AGCTCAAAGT 
CTTCCACCAG ATGAAATCGA GATCCTGTGC GGAGACGAGG CGGAAAAACT CCCGGAAAGA 
TCTTGCAGGA TGGAGAGCGG AGAGGTGGAT AACCGTAGAG CGGAGCACAG CAGGACGCTT 
CCTGACCGGG TGGTCGCACC GCTGCTGGCA GGGGGACCAG AGGTCAGGCT TCGCAGGTCT 
GAGATGAAGT TGTGGGGTCC AGACGCAGAT GGTGATGTGA AGGAGAAAAA GCGCAGCTCG 
TTGTTTTCAC CCCGTAAAAG CAGGAAGAAT GGAAATGCAG CAGCTGAGAG CGGGCGAGAG 
ACAGGGAAGC ACAAGTCCCT CTGGAAGACT GTTTTTTCAG TGTATAAGAA GGATAAAAAG 
AGGAAGGAGG TGGCAGTGGT GGCGGAAACA CTTCCTGCTG CTAATAATGG GAGCAAACGG 
AAAGTGTCGG GAATTAACAG AACCACAGAC CTGTGTTTCC GGAAGAATCC AAGTTTCTCT 
GAAGATACGG ATATGTCCTG TCATGCTCTT CTTGAAAGAT GTCCTCTCCG AGCTCAGAGA 
GCAGGAACAG AGGAGGAACT CAATGCCAGG CTGACACGTC GAGTGCAGAG AGCAGCGCGC 
AGACAGGCCA AGCAGGAGGA GCTGCGGAGG CTGCATAGAG CTCAGATAAT CCAGCGTCAG 
TTGGAGCAGG TGGAAGTGAA GCAGAGGCAG CTGGAGGAGA AAGGTGTGGC TGTGGAGAAA 
GCACTCAGAG GAGAAGCAGT TGAGCCTTTT GACGGTTCTC CCCGTAGAAG GCCTGTATAT 
CTCTGTCCTT GCTGTTCCTC TGAAGCCAAA GCTAAAGGAA CCGAACATGA TCATTCGGTT 
TATGTCTGTA CAGTCTTTTT CCTGTACGTA GTGCAGGAGC CTCCACTTCA CCAACTGAGA 
ATGGGAAAGA AAGACGATCC AAGCTTAATG CATCAGTGGT TCAAACTGGT GCAGGAGAAG 
AATGCTCTCG TGCGTTATGA ATCTGAGCTC ATGATATTCG CTCGTGAACT GGAGCTGGAG 
GACAGACAGA GTCGCCTGCA GCAGGAGCTG AGAGAGCGCA TGGCGGTGGA CGATCATCTA 
AAAGGAGAAG AGGAGCTGGC GGAGGAGAGG CGTATATTGA GCGAGATGCT GGATGTGGTG 
GAGCAGAGGG ATGCACTGGT GGCCCTCCTG GAGGAGCAGA GAGTTCGAGA AAAAGAGGAG 
GACAGTGACC TGGAGGCTGT GATGCTGTCT AAGGGCTTCA GTCTGCATTG GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( XM_021475218.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330893.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X7, mRNA.

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CloneID	ODa142126
Clone ID Related Accession (Same CDS sequence)	XM_021475218.1
Accession Version	XM_021475218.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTTAA AGGTGGCCTT GTTATTGGGT GTCGAAGTTC ATGTGAACGT GGAGTTCAAA 
CATCTACTGG AACCTCCAGA GAACCAGGAG AAACGTGTTG GTTGGCGGGC AGAGGTTCAG 
CCCAGCTCTC ATCCAGTCAG ACAGCTGGAG TTTGATGTGG TTATTGGGGC AGACGGCAGA 
AGAAACACAT TACCAGGGTT TCGCAGGAAG GAGTTTCGTG GGAAACTGGC CATTGCCATC 
ACGGCGAATT TCATCAATCG CAACACCACC GCCGAGGCTA AAGTGGAGGA GATCAGCGGC 
GTCGCCTTCA TTTTCAACCA GAGGTTTTTC CAGGACCTCC GGCAAGCTAC CGGAATCGAT 
CTGGAAAATA TAGTATACTA TAAAGACGAC ACTCACTATT TCGTCATGAC CGCCAAAAAA 
CAAAGTCTTC TGGAGAAAGG AGTCATCCTG CGTGATTATG CTGACACTGA GATGCTGCTG 
TCCAGACATA ATGTGGACCA GAATGCACTG CTCTCGTATG CACGTGAAGC GGCTGATTTC 
TCCACCAATC ATCAGCTTCC AGCGCTGGAT TTTGCCATCA ATCACTATGG GCAGTCAGAT 
GTGGCCATGT TTGACTTTAC CTGTATGTAC GCCTCGGAAA ACGCAGCGAT GGTACGTCAG 
CGTATGGGAC ATCCACTGCT CGTTGCTCTG GTGGGCGACA GTTTGCTGGA GCCGTTCTGG 
CCAATGGGAA CAGGGATAGC TCGGGGATTC CTGGCAGCGA TGGACACAGC GTGGATGGTG 
CGCAGCTGGG GTCAGGGCAA CACTCCTCTG GAGGTTCTGG CTGAGAGAGA GAGTGTGTAT 
AGGTTGCTTC CTCAGACAAC TCCAGAAAAT GTCAGTAAAA ACTACAGCCA GTTCAGTGTG 
GATCCTGCTA GTCGCTATCC CAACATTAAT ATGCAGCTCA TCAACGCCGC TCAGGTGCGT 
CACCTCATAG ACACAGGTGA GGGTCCAGTT TTGGGTCTCG ATGCTGTTAG TTCTCCACAC 
CCAAGACTTA CGCGACAAGA GTCAATGGCT CGGTACAGTA AACTGCTGAG CTGGTGTCAG 
GAACACACTC ATGGTTACAG GAAAGTGTGT GTGACGGACT TCACCTCTTC CTGGAGGAGC 
GGACTGGCCC TGTGTGCTCT CATTCACACC TTCAGGCCTG ACCTCATTGA TTTTGCATCT 
CTGGAGGAGA GCGAGGCAGA GTTCAGTGGT CAGCTGGGTT TGGACGTGGC GGAGCAGGAA 
TTTGGTATTT GTCCTATAAT GACCGGCAAG GAGATGAGTG TGCTGGAAGA GAGCGACTCT 
CTCTGTATGG TCATGTACCT CAGTCAGCTT CATGAGCTCC TCAAAGACAC ATCGCCACCT 
AGTGGAAGTC AGAGCTCAGA AGAGAGAGCG GTTTTGTTCT CCAGCAGCAG ATCACCAATC 
TCACTGCTCA GCAAACTGGG ACAGAGTTTG TCCCGCAAAC GCAATCCTAA GGATAAGAAA 
GAGAAGGAAG CAGACAGTGT TGGAAAGAGG AGGAGAACAA GTCAGGCCGG CCAATCGGAA 
GAGGAGGATG CTCTTCATGA CGGCAATGAA AATAAGTCTC CAGCAGAAAC TCCCGGCTCA 
GAACCGAAGG CCTCTGAGGG TCACAGCAAG GTTCGGTCCA TGGCGTCGCT CCTTCTGGCT 
AAATTTGAGG AGAACTCGCC ATCGCCTTCA ACCACTGCAA TACGCAGACA GAACTACATT 
CACATGTATA CCGGCGGAGT GAGCTCATTG GCTCTGCAGA TAGCCAATCA GATTCAAAGT 
CAGCAGGCTC AGGCTCCCAA GCTCCTTCAC AGGAGGGAGT CGGGTTCTCA GAAGGATCTT 
CCAGTAAATG TCGGCAGTAG TGATGTGTGT TATTTCTGTG GCCGTCGCGT TTATGTCATG 
GAGAGGCTGA GCGCAGAGGG AAAGTTCTTC CACAGGAGCT GCTTCCAGTG CGACCACTGC 
AGCTCCACGA TACGCCTCTC CAACTACACT TATGACCAGC TACATGGCAA GTTTTACTGT 
AAGCATCACT TCAGCTTCAG ACTGGCAAGT GTGGCTCAGA GAAAGAGACC GGCCCCACCG 
GTGGCCCCTC GGCCTGCTCA GGCTTCCCTG GCTGCATCCT CGGCCTCTAC CTCTTTGTCC 
TCTTTGGGCT CAGTGGGCAC AGCCACACCA GACAGCTGGT CCTCCAGCAC CCACACAGAC 
ATGGCATCCA GCTTGGCCAA GAGACTGTGC GGCACACCAG AACGCATTGA GCTTGAGAAT 
TACAAACCCT CCCCACAGAA ACAAGACAGC CCCCTGCAGG AGGTACCAGA GGAAACCCTC 
GCACAGCACA ATCTCAGCGC TAGCCTGCAG GAGAAGAATG CTGAAGAGCA GTCCAGTAGC 
TCTGAGTCAG ATCTAGAGGA GGAGGAGCTT GTCTGGAAGA AGGGGGAGGA GCTACATGCC 
AGGACCAATG GAGAGAGGAA GCTTGACCTA GAGGAGGAGC TGAAGGAAGA AGAGGGAGGG 
GAGAAGCTGG AAAAACAAGA GGGGGAGGAA GAAGGAGAAG TTTCAGAAGA AGAGCAAGAC 
GAGGGAGATT CTAGTGATGA GGGTGAGTAC TGCCCTTGGG AGAGGGAACG ACTCTCAGGG 
GTGTGGCTTG AGGAAGAGGA GGCGGGGTTT GTTAAAGAAT CTTATGAAGG ATGCAGCGAC 
GACCCTGATA TAGACGTCAG CTCACTCAGT GAGTCCAAGC TATGTGACCA ACGGGAACAG 
GAGGAGTCTG TTCCTTTCCA CCAATCCCCT GCCTCTACAG AATCTCCAGT CTCAATGAAA 
CCTTCAGAAT CAGACCTCAC ACCTGATCCG TCAACAACCC CCGAGAGCTC GCCTGCCAAG 
AGGTCAGAGG TGGTGGAGGA GTTCTGGATG AAGAGTGCAG AGATCCGAAA GAGTTTAGGC 
CTGACTCCGC TTTCTAGAGA ATTGCAGCCA AAACATATAG CAGCTTCAAC CCAGACATCT 
AATGTTAAAG AAAGCTTTTA CACCTCAGTC ACATATAACA CCGCTGCTTT GGATTCTCCA 
AACCAAAGTG CTAGAATCTG TGACTCCAGC ACACAAACTC ATTCTGTGAC AGATCTTCAA 
GAAACCTCCC CTCTTGGACC TACAGATGGC GATGCTGGCG TCGATTTAGG ACGTTGTTCT 
GTTGTTCACA GACTCAGCAT CACTGTAGAA GGTTGTGTGA TGGCAGATAA CCAGGGTTTG 
GATTCTACCT CGTTTGCTAC TGAAAGTGTT TTAAACCCAG ACGCAGGTCT TCCCACCCCT 
CCTTATAGTC CGTCTCACTC CCCTCTTGTT GGTAAACAGT GTCGTGCCCT TCACCATTCT 
GAGCCAATTC TAGACAGAGA GGTTATGGCT TTCTCATCTA CCTGCACTGC TTCTGTACCA 
CAGCGGAGCA GTTTTAAATC AGACCTCGAG CAAGCTCAAA GTCTTCCACC AGATGAAATC 
GAGATCCTGT GCGGAGACGA GGCGGAAAAA CTCCCGGAAA GATCTTGCAG GATGGAGAGC 
GGAGAGGTGG ATAACCGTAG AGCGGAGCAC AGCAGGACGC TTCCTGACCG GGTGGTCGCA 
CCGCTGCTGG CAGGGGGACC AGAGGTCAGG CTTCGCAGGT CTGAGATGAA GTTGTGGGGT 
CCAGACGCAG ATGGTGATGT GAAGGAGAAA AAGCGCAGCT CGTTGTTTTC ACCCCGTAAA 
AGCAGGAAGA ATGGAAATGC AGCAGCTGAG AGCGGGCGAG AGACAGGGAA GCACAAGTCC 
CTCTGGAAGA CTGTTTTTTC AGTGTATAAG AAGGATAAAA AGAGGAAGGA GGTGGCAGTG 
GTGGCGGAAA CACTTCCTGC TGCTAATAAT GGGAGCAAAC GGAAAGTGTC GGGAATTAAC 
AGAACCACAG ACCTGTGTTT CCGGAAGAAT CCAAGTTTCT CTGAAGATAC GGATATGTCC 
TGTCATGCTC TTCTTGAAAG ATGTCCTCTC CGAGCTCAGA GAGCAGGAAC AGAGGAGGAA 
CTCAATGCCA GGCTGACACG TCGAGTGCAG AGAGCAGCGC GCAGACAGGC CAAGCAGGAG 
GAGCTGCGGA GGCTGCATAG AGCTCAGATA ATCCAGCGTC AGTTGGAGCA GGTGGAAGTG 
AAGCAGAGGC AGCTGGAGGA GAAAGGTGTG GCTGTGGAGA AAGCACTCAG AGGAGAAGCA 
GTTGAGCCTT TTGACGGTTC TCCCCGTAGA AGGCCTGTAT ATCTCTGTCC TTGCTGTTCC 
TCTGAAGCCA AAGCTAAAGG AACCGAACAT GATCATTCGG TTTATGTCTG TACAGTCTTT 
TTCCTGTACG TAGTGCAGGA GCCTCCACTT CACCAACTGA GAATGGGAAA GAAAGACGAT 
CCAAGCTTAA TGCATCAGTG GTTCAAACTG GTGCAGGAGA AGAATGCTCT CGTGCGTTAT 
GAATCTGAGC TCATGATATT CGCTCGTGAA CTGGAGCTGG AGGACAGACA GAGTCGCCTG 
CAGCAGGAGC TGAGAGAGCG CATGGCGGTG GACGATCATC TAAAAGGAGA AGAGGAGCTG 
GCGGAGGAGA GGCGTATATT GAGCGAGATG CTGGATGTGG TGGAGCAGAG GGATGCACTG 
GTGGCCCTCC TGGAGGAGCA GAGAGTTCGA GAAAAAGAGG AGGACAGTGA CCTGGAGGCT 
GTGATGCTGT CTAAGGGCTT CAGTCTGCAT TGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330893.1
CDS	86..4804
Translation	MLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRKVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTAIRRQNYIHMYTGGVSSLALQIANQIQSQQAQAPKLLHRRESGSQKDLPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSSESDLEEEELVWKKGEELHARTNGERKLDLEEELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDEGEYCPWERERLSGVWLEEEEAGFVKESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNGSKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEAVEPFDGSPRRRPVYLCPCCSSEAKAKGTEHDHSVYVCTVFFLYVVQEPPLHQLRMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	XM_021475218.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475218.1

ORF Insert Sequence:

ATGCTTTTAA AGGTGGCCTT GTTATTGGGT GTCGAAGTTC ATGTGAACGT GGAGTTCAAA 
CATCTACTGG AACCTCCAGA GAACCAGGAG AAACGTGTTG GTTGGCGGGC AGAGGTTCAG 
CCCAGCTCTC ATCCAGTCAG ACAGCTGGAG TTTGATGTGG TTATTGGGGC AGACGGCAGA 
AGAAACACAT TACCAGGGTT TCGCAGGAAG GAGTTTCGTG GGAAACTGGC CATTGCCATC 
ACGGCGAATT TCATCAATCG CAACACCACC GCCGAGGCTA AAGTGGAGGA GATCAGCGGC 
GTCGCCTTCA TTTTCAACCA GAGGTTTTTC CAGGACCTCC GGCAAGCTAC CGGAATCGAT 
CTGGAAAATA TAGTATACTA TAAAGACGAC ACTCACTATT TCGTCATGAC CGCCAAAAAA 
CAAAGTCTTC TGGAGAAAGG AGTCATCCTG CGTGATTATG CTGACACTGA GATGCTGCTG 
TCCAGACATA ATGTGGACCA GAATGCACTG CTCTCGTATG CACGTGAAGC GGCTGATTTC 
TCCACCAATC ATCAGCTTCC AGCGCTGGAT TTTGCCATCA ATCACTATGG GCAGTCAGAT 
GTGGCCATGT TTGACTTTAC CTGTATGTAC GCCTCGGAAA ACGCAGCGAT GGTACGTCAG 
CGTATGGGAC ATCCACTGCT CGTTGCTCTG GTGGGCGACA GTTTGCTGGA GCCGTTCTGG 
CCAATGGGAA CAGGGATAGC TCGGGGATTC CTGGCAGCGA TGGACACAGC GTGGATGGTG 
CGCAGCTGGG GTCAGGGCAA CACTCCTCTG GAGGTTCTGG CTGAGAGAGA GAGTGTGTAT 
AGGTTGCTTC CTCAGACAAC TCCAGAAAAT GTCAGTAAAA ACTACAGCCA GTTCAGTGTG 
GATCCTGCTA GTCGCTATCC CAACATTAAT ATGCAGCTCA TCAACGCCGC TCAGGTGCGT 
CACCTCATAG ACACAGGTGA GGGTCCAGTT TTGGGTCTCG ATGCTGTTAG TTCTCCACAC 
CCAAGACTTA CGCGACAAGA GTCAATGGCT CGGTACAGTA AACTGCTGAG CTGGTGTCAG 
GAACACACTC ATGGTTACAG GAAAGTGTGT GTGACGGACT TCACCTCTTC CTGGAGGAGC 
GGACTGGCCC TGTGTGCTCT CATTCACACC TTCAGGCCTG ACCTCATTGA TTTTGCATCT 
CTGGAGGAGA GCGAGGCAGA GTTCAGTGGT CAGCTGGGTT TGGACGTGGC GGAGCAGGAA 
TTTGGTATTT GTCCTATAAT GACCGGCAAG GAGATGAGTG TGCTGGAAGA GAGCGACTCT 
CTCTGTATGG TCATGTACCT CAGTCAGCTT CATGAGCTCC TCAAAGACAC ATCGCCACCT 
AGTGGAAGTC AGAGCTCAGA AGAGAGAGCG GTTTTGTTCT CCAGCAGCAG ATCACCAATC 
TCACTGCTCA GCAAACTGGG ACAGAGTTTG TCCCGCAAAC GCAATCCTAA GGATAAGAAA 
GAGAAGGAAG CAGACAGTGT TGGAAAGAGG AGGAGAACAA GTCAGGCCGG CCAATCGGAA 
GAGGAGGATG CTCTTCATGA CGGCAATGAA AATAAGTCTC CAGCAGAAAC TCCCGGCTCA 
GAACCGAAGG CCTCTGAGGG TCACAGCAAG GTTCGGTCCA TGGCGTCGCT CCTTCTGGCT 
AAATTTGAGG AGAACTCGCC ATCGCCTTCA ACCACTGCAA TACGCAGACA GAACTACATT 
CACATGTATA CCGGCGGAGT GAGCTCATTG GCTCTGCAGA TAGCCAATCA GATTCAAAGT 
CAGCAGGCTC AGGCTCCCAA GCTCCTTCAC AGGAGGGAGT CGGGTTCTCA GAAGGATCTT 
CCAGTAAATG TCGGCAGTAG TGATGTGTGT TATTTCTGTG GCCGTCGCGT TTATGTCATG 
GAGAGGCTGA GCGCAGAGGG AAAGTTCTTC CACAGGAGCT GCTTCCAGTG CGACCACTGC 
AGCTCCACGA TACGCCTCTC CAACTACACT TATGACCAGC TACATGGCAA GTTTTACTGT 
AAGCATCACT TCAGCTTCAG ACTGGCAAGT GTGGCTCAGA GAAAGAGACC GGCCCCACCG 
GTGGCCCCTC GGCCTGCTCA GGCTTCCCTG GCTGCATCCT CGGCCTCTAC CTCTTTGTCC 
TCTTTGGGCT CAGTGGGCAC AGCCACACCA GACAGCTGGT CCTCCAGCAC CCACACAGAC 
ATGGCATCCA GCTTGGCCAA GAGACTGTGC GGCACACCAG AACGCATTGA GCTTGAGAAT 
TACAAACCCT CCCCACAGAA ACAAGACAGC CCCCTGCAGG AGGTACCAGA GGAAACCCTC 
GCACAGCACA ATCTCAGCGC TAGCCTGCAG GAGAAGAATG CTGAAGAGCA GTCCAGTAGC 
TCTGAGTCAG ATCTAGAGGA GGAGGAGCTT GTCTGGAAGA AGGGGGAGGA GCTACATGCC 
AGGACCAATG GAGAGAGGAA GCTTGACCTA GAGGAGGAGC TGAAGGAAGA AGAGGGAGGG 
GAGAAGCTGG AAAAACAAGA GGGGGAGGAA GAAGGAGAAG TTTCAGAAGA AGAGCAAGAC 
GAGGGAGATT CTAGTGATGA GGGTGAGTAC TGCCCTTGGG AGAGGGAACG ACTCTCAGGG 
GTGTGGCTTG AGGAAGAGGA GGCGGGGTTT GTTAAAGAAT CTTATGAAGG ATGCAGCGAC 
GACCCTGATA TAGACGTCAG CTCACTCAGT GAGTCCAAGC TATGTGACCA ACGGGAACAG 
GAGGAGTCTG TTCCTTTCCA CCAATCCCCT GCCTCTACAG AATCTCCAGT CTCAATGAAA 
CCTTCAGAAT CAGACCTCAC ACCTGATCCG TCAACAACCC CCGAGAGCTC GCCTGCCAAG 
AGGTCAGAGG TGGTGGAGGA GTTCTGGATG AAGAGTGCAG AGATCCGAAA GAGTTTAGGC 
CTGACTCCGC TTTCTAGAGA ATTGCAGCCA AAACATATAG CAGCTTCAAC CCAGACATCT 
AATGTTAAAG AAAGCTTTTA CACCTCAGTC ACATATAACA CCGCTGCTTT GGATTCTCCA 
AACCAAAGTG CTAGAATCTG TGACTCCAGC ACACAAACTC ATTCTGTGAC AGATCTTCAA 
GAAACCTCCC CTCTTGGACC TACAGATGGC GATGCTGGCG TCGATTTAGG ACGTTGTTCT 
GTTGTTCACA GACTCAGCAT CACTGTAGAA GGTTGTGTGA TGGCAGATAA CCAGGGTTTG 
GATTCTACCT CGTTTGCTAC TGAAAGTGTT TTAAACCCAG ACGCAGGTCT TCCCACCCCT 
CCTTATAGTC CGTCTCACTC CCCTCTTGTT GGTAAACAGT GTCGTGCCCT TCACCATTCT 
GAGCCAATTC TAGACAGAGA GGTTATGGCT TTCTCATCTA CCTGCACTGC TTCTGTACCA 
CAGCGGAGCA GTTTTAAATC AGACCTCGAG CAAGCTCAAA GTCTTCCACC AGATGAAATC 
GAGATCCTGT GCGGAGACGA GGCGGAAAAA CTCCCGGAAA GATCTTGCAG GATGGAGAGC 
GGAGAGGTGG ATAACCGTAG AGCGGAGCAC AGCAGGACGC TTCCTGACCG GGTGGTCGCA 
CCGCTGCTGG CAGGGGGACC AGAGGTCAGG CTTCGCAGGT CTGAGATGAA GTTGTGGGGT 
CCAGACGCAG ATGGTGATGT GAAGGAGAAA AAGCGCAGCT CGTTGTTTTC ACCCCGTAAA 
AGCAGGAAGA ATGGAAATGC AGCAGCTGAG AGCGGGCGAG AGACAGGGAA GCACAAGTCC 
CTCTGGAAGA CTGTTTTTTC AGTGTATAAG AAGGATAAAA AGAGGAAGGA GGTGGCAGTG 
GTGGCGGAAA CACTTCCTGC TGCTAATAAT GGGAGCAAAC GGAAAGTGTC GGGAATTAAC 
AGAACCACAG ACCTGTGTTT CCGGAAGAAT CCAAGTTTCT CTGAAGATAC GGATATGTCC 
TGTCATGCTC TTCTTGAAAG ATGTCCTCTC CGAGCTCAGA GAGCAGGAAC AGAGGAGGAA 
CTCAATGCCA GGCTGACACG TCGAGTGCAG AGAGCAGCGC GCAGACAGGC CAAGCAGGAG 
GAGCTGCGGA GGCTGCATAG AGCTCAGATA ATCCAGCGTC AGTTGGAGCA GGTGGAAGTG 
AAGCAGAGGC AGCTGGAGGA GAAAGGTGTG GCTGTGGAGA AAGCACTCAG AGGAGAAGCA 
GTTGAGCCTT TTGACGGTTC TCCCCGTAGA AGGCCTGTAT ATCTCTGTCC TTGCTGTTCC 
TCTGAAGCCA AAGCTAAAGG AACCGAACAT GATCATTCGG TTTATGTCTG TACAGTCTTT 
TTCCTGTACG TAGTGCAGGA GCCTCCACTT CACCAACTGA GAATGGGAAA GAAAGACGAT 
CCAAGCTTAA TGCATCAGTG GTTCAAACTG GTGCAGGAGA AGAATGCTCT CGTGCGTTAT 
GAATCTGAGC TCATGATATT CGCTCGTGAA CTGGAGCTGG AGGACAGACA GAGTCGCCTG 
CAGCAGGAGC TGAGAGAGCG CATGGCGGTG GACGATCATC TAAAAGGAGA AGAGGAGCTG 
GCGGAGGAGA GGCGTATATT GAGCGAGATG CTGGATGTGG TGGAGCAGAG GGATGCACTG 
GTGGCCCTCC TGGAGGAGCA GAGAGTTCGA GAAAAAGAGG AGGACAGTGA CCTGGAGGCT 
GTGATGCTGT CTAAGGGCTT CAGTCTGCAT TGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( XM_021473992.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329667.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X1, mRNA.

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CloneID	ODa146493
Clone ID Related Accession (Same CDS sequence)	XM_021473992.1
Accession Version	XM_021473992.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395230.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCTGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTAAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATTAATCGC AATACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AACGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTCGCTCTGG TGGGAGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGCTTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG CCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AACGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCACT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT ACTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCCGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC TTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTTGCTA AATTTGAAGA GAACTCGCCA TCGCCTTCAA CCACTGCAAC ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCAGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGAGTTTC CAGTAAATGT CGGCAGCAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTAAG CGCAGAGGGC AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGGCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGTTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGATGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAG GGTGAGTACT GCCCTTGGGA GAGGGAACGA 
CTCTCAGGGG TGTGGCTTGA GGAAGAGGAG GCGGGGTTTG TTAAAGAATC TTATGAAGGA 
TGCAGCGACG ACCCTGATAT AGACGTCAGC TCACTCAGTG AGTCCAAGCT ATGTGACCAA 
CGGGAACAGG AGGAGTCGGT TCCTTTCCAC CAATCCCCTG CCTCTACAGA ATCTCCAGTC 
TCAATGAAAC CTTCAGAATC AGACCTCACA CCTGATCCGT CAACAACCCC CGAGAGCTCG 
CCTGCCAAGA GGTCAGAGGT GGTGGAGGAG TTCTGGATGA AGAGTGCAGA GATCAGAAAG 
AGTTTAGGCC TGACTCCGCT TTCTAGAGAA TTGCAGCCAA AACATATAGC AGCTTCAACC 
CAGACATCTA ATGTTAAAGA AAGCTTTTAC ACCTCAGTCA CATATAACAC CGCTGCTTTG 
GATTCTCCAA ACCAAAGTGC TAGAATCTGT GACTCCAGCA CACAAACTCA TTCTGTGACA 
GATCTTCAAG AAACCTCCCC TCTTGGACCT ACAGATGGCG ATGCTGGCGT CGATTTAGGA 
CGTTGTTCTG TTGTTCACAG ACTCAGCATC ACTGTAGAAG GTTGTGTGAT GGCAGATAAC 
CAGGGTTTGG ATTCTACCTC GTTTGCTACT GAAAGTGTTT TAAACCCAGA CGCAGGTCTT 
CCCACCCCTC CTTATAGTCC GTCTCACTCC CCTCTTGTTG GTAAACAGTG TCGTGCCCTT 
CACCATTCTG AGCCAATTCT AGACAGAGAG GTTATGGCTT TCTCATCTAC CTGCACTGCT 
TCTGTACCAC AGCGGAGCAG TTTTAAATCA GACCTCGAGC AAGCTCAAAG TCTTCCACCA 
GATGAAATCG AGATCCTGTG CGGAGACGAG GCGGAAAAAC TCCCGGAAAG ATCTTGCAGG 
ATGGAGAGCG GAGAGGTGGA TAACCGTAGA GCGGAGCACA GCAGGACGCT TCCTGACCGG 
GTGGTCGCAC CGCTGCTGGC AGGGGGACCA GAGGTCAGGC TTCGCAGGTC TGAGATGAAG 
TTGTGGGGTC CAGACGCAGA TGGTGATGTG AAGGAGAAAA AGCGCAGCTC GTTGTTTTCA 
CCCCGTAAAA GCAGGAAGAA TGGAAATGCA GCAGCTGAGA GCGGGCGAGA GACAGGGAAG 
CACAAGTCCC TCTGGAAGAC TGTTTTTTCA GTGTATAAGA AGGATAAAAA GAGGAAGGAG 
GTGGCAGTGG TGGCGGAAAC ACTTCCTGCT GCTAATAATG AGAGCAAACG GAAAGTGTCG 
GGAATCAACA GAACCACAGA CCTGTGTTTC CGGAAGAATC CAAGTTTCTC TGAAGATACG 
GATATGTCCT GTCATGCTCT TCTTGAAAGA TGTCCTCTCC GAGCTCAGAG AGCAGGAACA 
GAGGAGGAAC TCAATGCCAG GCTGACACGT CGAGTGCAGA GAGCAGCGCG CAGACAGGCC 
AAGCAGGAGG AGCTGCGGAG GCTGCATAGA GCTCAGATAA TCCAGCGTCA GTTGGAGCAG 
GTGGAAGTGA AGCAGAGGCA GCTGGAGGAG AAAGGTGTGG CTGTGGAGAA AGCACTCAGA 
GGAGAAGCAG TTGAGCCTTT TGACGGTTCT CCCCGTAGAA GGCCTGTATA TCTCTGTCCT 
TGCTGTTCCT CTGAAGCCAA AGCTAAAGGA ACCGAACATG ATCATTCGGT TTATGTCTGT 
ACAGTCTTTT TCCTGTACGT AGTGCAGGAG CCTCCACTTC ACCAACTGAG AATGGGAAAG 
AAAGACGATC CAAGCTTAAT GCATCAGTGG TTCAAACTGG TGCAGGAGAA GAATGCTCTC 
GTGCGTTATG AATCTGAGCT CATGATATTC GCTCGTGAAC TGGAGCTGGA GGACAGACAG 
AGTCGCCTGC AGCAGGAGCT GAGAGAGCGC ATGGCGGTGG ACGATCATCT AAAAGGAGAA 
GAGGAGCTGG CGGAGGAGAG GCGTATATTG AGCGAGATGC TGGATGTGGT GGAGCAGAGG 
GATGCACTGG TGGCCCTCCT GGAGGAGCAG AGAGTTCGAG AAAAAGAGGA GGACAGTGAC 
CTGGAGGCTG TGATGCTGTC TAAGGGCTTC AGTCTGCATT GGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329667.1
CDS	598..5805
Translation	MWDGQSEMCQAHVLFDSFVQAATCKETLRAFQDLCEELNLNPCGQPQFYHTLRSRLHYWKAKALWAKLDKRACQKEYMRGHACTSTTCLIIGAGPCGLRTAIELGFLGARVVLVEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRNVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTATRRQNYIHMYTGGVSSLAQQIANQIQSQQAQAPKLLHRRESGSQKEFPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSSESDLEEEELVWKKGEELHARTNGERKLDLEDELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDEGEYCPWERERLSGVWLEEEEAGFVKESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNESKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEAVEPFDGSPRRRPVYLCPCCSSEAKAKGTEHDHSVYVCTVFFLYVVQEPPLHQLRMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	XM_021473992.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473992.1

ORF Insert Sequence:

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCTGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTAAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATTAATCGC AATACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AACGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTCGCTCTGG TGGGAGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGCTTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG CCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AACGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCACT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT ACTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCCGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC TTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTTGCTA AATTTGAAGA GAACTCGCCA TCGCCTTCAA CCACTGCAAC ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCAGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGAGTTTC CAGTAAATGT CGGCAGCAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTAAG CGCAGAGGGC AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGGCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGTTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGATGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAG GGTGAGTACT GCCCTTGGGA GAGGGAACGA 
CTCTCAGGGG TGTGGCTTGA GGAAGAGGAG GCGGGGTTTG TTAAAGAATC TTATGAAGGA 
TGCAGCGACG ACCCTGATAT AGACGTCAGC TCACTCAGTG AGTCCAAGCT ATGTGACCAA 
CGGGAACAGG AGGAGTCGGT TCCTTTCCAC CAATCCCCTG CCTCTACAGA ATCTCCAGTC 
TCAATGAAAC CTTCAGAATC AGACCTCACA CCTGATCCGT CAACAACCCC CGAGAGCTCG 
CCTGCCAAGA GGTCAGAGGT GGTGGAGGAG TTCTGGATGA AGAGTGCAGA GATCAGAAAG 
AGTTTAGGCC TGACTCCGCT TTCTAGAGAA TTGCAGCCAA AACATATAGC AGCTTCAACC 
CAGACATCTA ATGTTAAAGA AAGCTTTTAC ACCTCAGTCA CATATAACAC CGCTGCTTTG 
GATTCTCCAA ACCAAAGTGC TAGAATCTGT GACTCCAGCA CACAAACTCA TTCTGTGACA 
GATCTTCAAG AAACCTCCCC TCTTGGACCT ACAGATGGCG ATGCTGGCGT CGATTTAGGA 
CGTTGTTCTG TTGTTCACAG ACTCAGCATC ACTGTAGAAG GTTGTGTGAT GGCAGATAAC 
CAGGGTTTGG ATTCTACCTC GTTTGCTACT GAAAGTGTTT TAAACCCAGA CGCAGGTCTT 
CCCACCCCTC CTTATAGTCC GTCTCACTCC CCTCTTGTTG GTAAACAGTG TCGTGCCCTT 
CACCATTCTG AGCCAATTCT AGACAGAGAG GTTATGGCTT TCTCATCTAC CTGCACTGCT 
TCTGTACCAC AGCGGAGCAG TTTTAAATCA GACCTCGAGC AAGCTCAAAG TCTTCCACCA 
GATGAAATCG AGATCCTGTG CGGAGACGAG GCGGAAAAAC TCCCGGAAAG ATCTTGCAGG 
ATGGAGAGCG GAGAGGTGGA TAACCGTAGA GCGGAGCACA GCAGGACGCT TCCTGACCGG 
GTGGTCGCAC CGCTGCTGGC AGGGGGACCA GAGGTCAGGC TTCGCAGGTC TGAGATGAAG 
TTGTGGGGTC CAGACGCAGA TGGTGATGTG AAGGAGAAAA AGCGCAGCTC GTTGTTTTCA 
CCCCGTAAAA GCAGGAAGAA TGGAAATGCA GCAGCTGAGA GCGGGCGAGA GACAGGGAAG 
CACAAGTCCC TCTGGAAGAC TGTTTTTTCA GTGTATAAGA AGGATAAAAA GAGGAAGGAG 
GTGGCAGTGG TGGCGGAAAC ACTTCCTGCT GCTAATAATG AGAGCAAACG GAAAGTGTCG 
GGAATCAACA GAACCACAGA CCTGTGTTTC CGGAAGAATC CAAGTTTCTC TGAAGATACG 
GATATGTCCT GTCATGCTCT TCTTGAAAGA TGTCCTCTCC GAGCTCAGAG AGCAGGAACA 
GAGGAGGAAC TCAATGCCAG GCTGACACGT CGAGTGCAGA GAGCAGCGCG CAGACAGGCC 
AAGCAGGAGG AGCTGCGGAG GCTGCATAGA GCTCAGATAA TCCAGCGTCA GTTGGAGCAG 
GTGGAAGTGA AGCAGAGGCA GCTGGAGGAG AAAGGTGTGG CTGTGGAGAA AGCACTCAGA 
GGAGAAGCAG TTGAGCCTTT TGACGGTTCT CCCCGTAGAA GGCCTGTATA TCTCTGTCCT 
TGCTGTTCCT CTGAAGCCAA AGCTAAAGGA ACCGAACATG ATCATTCGGT TTATGTCTGT 
ACAGTCTTTT TCCTGTACGT AGTGCAGGAG CCTCCACTTC ACCAACTGAG AATGGGAAAG 
AAAGACGATC CAAGCTTAAT GCATCAGTGG TTCAAACTGG TGCAGGAGAA GAATGCTCTC 
GTGCGTTATG AATCTGAGCT CATGATATTC GCTCGTGAAC TGGAGCTGGA GGACAGACAG 
AGTCGCCTGC AGCAGGAGCT GAGAGAGCGC ATGGCGGTGG ACGATCATCT AAAAGGAGAA 
GAGGAGCTGG CGGAGGAGAG GCGTATATTG AGCGAGATGC TGGATGTGGT GGAGCAGAGG 
GATGCACTGG TGGCCCTCCT GGAGGAGCAG AGAGTTCGAG AAAAAGAGGA GGACAGTGAC 
CTGGAGGCTG TGATGCTGTC TAAGGGCTTC AGTCTGCATT GGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( XM_021473993.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329668.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X2, mRNA.

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CloneID	ODa146494
Clone ID Related Accession (Same CDS sequence)	XM_021473993.1
Accession Version	XM_021473993.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395230.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCTGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTAAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATTAATCGC AATACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AACGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTCGCTCTGG TGGGAGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGCTTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG CCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AACGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCACT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT ACTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCCGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC TTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTTGCTA AATTTGAAGA GAACTCGCCA TCGCCTTCAA CCACTGCAAC ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCAGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGAGTTTC CAGTAAATGT CGGCAGCAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTAAG CGCAGAGGGC AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGGCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGTTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGCTCTG AGTCAGATCT AGAGGAGGAG GAGCTTGTCT GGAAGAAGGG GGAGGAGCTA 
CATGCCAGGA CCAATGGAGA GAGGAAGCTT GACCTAGAGG ATGAGCTGAA GGAAGAAGAG 
GGAGGGGAGA AGCTGGAAAA ACAAGAGGGG GAGGAAGAAG GAGAAGTTTC AGAAGAAGAG 
CAAGACGAGG GAGATTCTAG TGATGAGGGT GAGTACTGCC CTTGGGAGAG GGAACGACTC 
TCAGGGGTGT GGCTTGAGGA AGAGGAGGCG GGGTTTGTTA AAGAATCTTA TGAAGGATGC 
AGCGACGACC CTGATATAGA CGTCAGCTCA CTCAGTGAGT CCAAGCTATG TGACCAACGG 
GAACAGGAGG AGTCGGTTCC TTTCCACCAA TCCCCTGCCT CTACAGAATC TCCAGTCTCA 
ATGAAACCTT CAGAATCAGA CCTCACACCT GATCCGTCAA CAACCCCCGA GAGCTCGCCT 
GCCAAGAGGT CAGAGGTGGT GGAGGAGTTC TGGATGAAGA GTGCAGAGAT CAGAAAGAGT 
TTAGGCCTGA CTCCGCTTTC TAGAGAATTG CAGCCAAAAC ATATAGCAGC TTCAACCCAG 
ACATCTAATG TTAAAGAAAG CTTTTACACC TCAGTCACAT ATAACACCGC TGCTTTGGAT 
TCTCCAAACC AAAGTGCTAG AATCTGTGAC TCCAGCACAC AAACTCATTC TGTGACAGAT 
CTTCAAGAAA CCTCCCCTCT TGGACCTACA GATGGCGATG CTGGCGTCGA TTTAGGACGT 
TGTTCTGTTG TTCACAGACT CAGCATCACT GTAGAAGGTT GTGTGATGGC AGATAACCAG 
GGTTTGGATT CTACCTCGTT TGCTACTGAA AGTGTTTTAA ACCCAGACGC AGGTCTTCCC 
ACCCCTCCTT ATAGTCCGTC TCACTCCCCT CTTGTTGGTA AACAGTGTCG TGCCCTTCAC 
CATTCTGAGC CAATTCTAGA CAGAGAGGTT ATGGCTTTCT CATCTACCTG CACTGCTTCT 
GTACCACAGC GGAGCAGTTT TAAATCAGAC CTCGAGCAAG CTCAAAGTCT TCCACCAGAT 
GAAATCGAGA TCCTGTGCGG AGACGAGGCG GAAAAACTCC CGGAAAGATC TTGCAGGATG 
GAGAGCGGAG AGGTGGATAA CCGTAGAGCG GAGCACAGCA GGACGCTTCC TGACCGGGTG 
GTCGCACCGC TGCTGGCAGG GGGACCAGAG GTCAGGCTTC GCAGGTCTGA GATGAAGTTG 
TGGGGTCCAG ACGCAGATGG TGATGTGAAG GAGAAAAAGC GCAGCTCGTT GTTTTCACCC 
CGTAAAAGCA GGAAGAATGG AAATGCAGCA GCTGAGAGCG GGCGAGAGAC AGGGAAGCAC 
AAGTCCCTCT GGAAGACTGT TTTTTCAGTG TATAAGAAGG ATAAAAAGAG GAAGGAGGTG 
GCAGTGGTGG CGGAAACACT TCCTGCTGCT AATAATGAGA GCAAACGGAA AGTGTCGGGA 
ATCAACAGAA CCACAGACCT GTGTTTCCGG AAGAATCCAA GTTTCTCTGA AGATACGGAT 
ATGTCCTGTC ATGCTCTTCT TGAAAGATGT CCTCTCCGAG CTCAGAGAGC AGGAACAGAG 
GAGGAACTCA ATGCCAGGCT GACACGTCGA GTGCAGAGAG CAGCGCGCAG ACAGGCCAAG 
CAGGAGGAGC TGCGGAGGCT GCATAGAGCT CAGATAATCC AGCGTCAGTT GGAGCAGGTG 
GAAGTGAAGC AGAGGCAGCT GGAGGAGAAA GGTGTGGCTG TGGAGAAAGC ACTCAGAGGA 
GAAGCAGTTG AGCCTTTTGA CGGTTCTCCC CGTAGAAGGC CTGTATATCT CTGTCCTTGC 
TGTTCCTCTG AAGCCAAAGC TAAAGGAACC GAACATGATC ATTCGGTTTA TGTCTGTACA 
GTCTTTTTCC TGTACGTAGT GCAGGAGCCT CCACTTCACC AACTGAGAAT GGGAAAGAAA 
GACGATCCAA GCTTAATGCA TCAGTGGTTC AAACTGGTGC AGGAGAAGAA TGCTCTCGTG 
CGTTATGAAT CTGAGCTCAT GATATTCGCT CGTGAACTGG AGCTGGAGGA CAGACAGAGT 
CGCCTGCAGC AGGAGCTGAG AGAGCGCATG GCGGTGGACG ATCATCTAAA AGGAGAAGAG 
GAGCTGGCGG AGGAGAGGCG TATATTGAGC GAGATGCTGG ATGTGGTGGA GCAGAGGGAT 
GCACTGGTGG CCCTCCTGGA GGAGCAGAGA GTTCGAGAAA AAGAGGAGGA CAGTGACCTG 
GAGGCTGTGA TGCTGTCTAA GGGCTTCAGT CTGCATTGGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329668.1
CDS	598..5802
Translation	MWDGQSEMCQAHVLFDSFVQAATCKETLRAFQDLCEELNLNPCGQPQFYHTLRSRLHYWKAKALWAKLDKRACQKEYMRGHACTSTTCLIIGAGPCGLRTAIELGFLGARVVLVEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRNVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTATRRQNYIHMYTGGVSSLAQQIANQIQSQQAQAPKLLHRRESGSQKEFPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSESDLEEEELVWKKGEELHARTNGERKLDLEDELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDEGEYCPWERERLSGVWLEEEEAGFVKESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNESKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEAVEPFDGSPRRRPVYLCPCCSSEAKAKGTEHDHSVYVCTVFFLYVVQEPPLHQLRMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	XM_021473993.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473993.1

ORF Insert Sequence:

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCTGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTAAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATTAATCGC AATACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AACGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTCGCTCTGG TGGGAGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGCTTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG CCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AACGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCACT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT ACTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCCGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC TTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTTGCTA AATTTGAAGA GAACTCGCCA TCGCCTTCAA CCACTGCAAC ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCAGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGAGTTTC CAGTAAATGT CGGCAGCAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTAAG CGCAGAGGGC AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGGCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGTTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGCTCTG AGTCAGATCT AGAGGAGGAG GAGCTTGTCT GGAAGAAGGG GGAGGAGCTA 
CATGCCAGGA CCAATGGAGA GAGGAAGCTT GACCTAGAGG ATGAGCTGAA GGAAGAAGAG 
GGAGGGGAGA AGCTGGAAAA ACAAGAGGGG GAGGAAGAAG GAGAAGTTTC AGAAGAAGAG 
CAAGACGAGG GAGATTCTAG TGATGAGGGT GAGTACTGCC CTTGGGAGAG GGAACGACTC 
TCAGGGGTGT GGCTTGAGGA AGAGGAGGCG GGGTTTGTTA AAGAATCTTA TGAAGGATGC 
AGCGACGACC CTGATATAGA CGTCAGCTCA CTCAGTGAGT CCAAGCTATG TGACCAACGG 
GAACAGGAGG AGTCGGTTCC TTTCCACCAA TCCCCTGCCT CTACAGAATC TCCAGTCTCA 
ATGAAACCTT CAGAATCAGA CCTCACACCT GATCCGTCAA CAACCCCCGA GAGCTCGCCT 
GCCAAGAGGT CAGAGGTGGT GGAGGAGTTC TGGATGAAGA GTGCAGAGAT CAGAAAGAGT 
TTAGGCCTGA CTCCGCTTTC TAGAGAATTG CAGCCAAAAC ATATAGCAGC TTCAACCCAG 
ACATCTAATG TTAAAGAAAG CTTTTACACC TCAGTCACAT ATAACACCGC TGCTTTGGAT 
TCTCCAAACC AAAGTGCTAG AATCTGTGAC TCCAGCACAC AAACTCATTC TGTGACAGAT 
CTTCAAGAAA CCTCCCCTCT TGGACCTACA GATGGCGATG CTGGCGTCGA TTTAGGACGT 
TGTTCTGTTG TTCACAGACT CAGCATCACT GTAGAAGGTT GTGTGATGGC AGATAACCAG 
GGTTTGGATT CTACCTCGTT TGCTACTGAA AGTGTTTTAA ACCCAGACGC AGGTCTTCCC 
ACCCCTCCTT ATAGTCCGTC TCACTCCCCT CTTGTTGGTA AACAGTGTCG TGCCCTTCAC 
CATTCTGAGC CAATTCTAGA CAGAGAGGTT ATGGCTTTCT CATCTACCTG CACTGCTTCT 
GTACCACAGC GGAGCAGTTT TAAATCAGAC CTCGAGCAAG CTCAAAGTCT TCCACCAGAT 
GAAATCGAGA TCCTGTGCGG AGACGAGGCG GAAAAACTCC CGGAAAGATC TTGCAGGATG 
GAGAGCGGAG AGGTGGATAA CCGTAGAGCG GAGCACAGCA GGACGCTTCC TGACCGGGTG 
GTCGCACCGC TGCTGGCAGG GGGACCAGAG GTCAGGCTTC GCAGGTCTGA GATGAAGTTG 
TGGGGTCCAG ACGCAGATGG TGATGTGAAG GAGAAAAAGC GCAGCTCGTT GTTTTCACCC 
CGTAAAAGCA GGAAGAATGG AAATGCAGCA GCTGAGAGCG GGCGAGAGAC AGGGAAGCAC 
AAGTCCCTCT GGAAGACTGT TTTTTCAGTG TATAAGAAGG ATAAAAAGAG GAAGGAGGTG 
GCAGTGGTGG CGGAAACACT TCCTGCTGCT AATAATGAGA GCAAACGGAA AGTGTCGGGA 
ATCAACAGAA CCACAGACCT GTGTTTCCGG AAGAATCCAA GTTTCTCTGA AGATACGGAT 
ATGTCCTGTC ATGCTCTTCT TGAAAGATGT CCTCTCCGAG CTCAGAGAGC AGGAACAGAG 
GAGGAACTCA ATGCCAGGCT GACACGTCGA GTGCAGAGAG CAGCGCGCAG ACAGGCCAAG 
CAGGAGGAGC TGCGGAGGCT GCATAGAGCT CAGATAATCC AGCGTCAGTT GGAGCAGGTG 
GAAGTGAAGC AGAGGCAGCT GGAGGAGAAA GGTGTGGCTG TGGAGAAAGC ACTCAGAGGA 
GAAGCAGTTG AGCCTTTTGA CGGTTCTCCC CGTAGAAGGC CTGTATATCT CTGTCCTTGC 
TGTTCCTCTG AAGCCAAAGC TAAAGGAACC GAACATGATC ATTCGGTTTA TGTCTGTACA 
GTCTTTTTCC TGTACGTAGT GCAGGAGCCT CCACTTCACC AACTGAGAAT GGGAAAGAAA 
GACGATCCAA GCTTAATGCA TCAGTGGTTC AAACTGGTGC AGGAGAAGAA TGCTCTCGTG 
CGTTATGAAT CTGAGCTCAT GATATTCGCT CGTGAACTGG AGCTGGAGGA CAGACAGAGT 
CGCCTGCAGC AGGAGCTGAG AGAGCGCATG GCGGTGGACG ATCATCTAAA AGGAGAAGAG 
GAGCTGGCGG AGGAGAGGCG TATATTGAGC GAGATGCTGG ATGTGGTGGA GCAGAGGGAT 
GCACTGGTGG CCCTCCTGGA GGAGCAGAGA GTTCGAGAAA AAGAGGAGGA CAGTGACCTG 
GAGGCTGTGA TGCTGTCTAA GGGCTTCAGT CTGCATTGGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( XM_021473994.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329669.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa146495
Clone ID Related Accession (Same CDS sequence)	XM_021473994.1
Accession Version	XM_021473994.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395230.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCTGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTAAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATTAATCGC AATACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AACGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTCGCTCTGG TGGGAGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGCTTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG CCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AACGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCACT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT ACTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCCGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC TTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTTGCTA AATTTGAAGA GAACTCGCCA TCGCCTTCAA CCACTGCAAC ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCAGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGAGTTTC CAGTAAATGT CGGCAGCAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTAAG CGCAGAGGGC AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGGCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGTTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGATGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAA TCTTATGAAG GATGCAGCGA CGACCCTGAT 
ATAGACGTCA GCTCACTCAG TGAGTCCAAG CTATGTGACC AACGGGAACA GGAGGAGTCG 
GTTCCTTTCC ACCAATCCCC TGCCTCTACA GAATCTCCAG TCTCAATGAA ACCTTCAGAA 
TCAGACCTCA CACCTGATCC GTCAACAACC CCCGAGAGCT CGCCTGCCAA GAGGTCAGAG 
GTGGTGGAGG AGTTCTGGAT GAAGAGTGCA GAGATCAGAA AGAGTTTAGG CCTGACTCCG 
CTTTCTAGAG AATTGCAGCC AAAACATATA GCAGCTTCAA CCCAGACATC TAATGTTAAA 
GAAAGCTTTT ACACCTCAGT CACATATAAC ACCGCTGCTT TGGATTCTCC AAACCAAAGT 
GCTAGAATCT GTGACTCCAG CACACAAACT CATTCTGTGA CAGATCTTCA AGAAACCTCC 
CCTCTTGGAC CTACAGATGG CGATGCTGGC GTCGATTTAG GACGTTGTTC TGTTGTTCAC 
AGACTCAGCA TCACTGTAGA AGGTTGTGTG ATGGCAGATA ACCAGGGTTT GGATTCTACC 
TCGTTTGCTA CTGAAAGTGT TTTAAACCCA GACGCAGGTC TTCCCACCCC TCCTTATAGT 
CCGTCTCACT CCCCTCTTGT TGGTAAACAG TGTCGTGCCC TTCACCATTC TGAGCCAATT 
CTAGACAGAG AGGTTATGGC TTTCTCATCT ACCTGCACTG CTTCTGTACC ACAGCGGAGC 
AGTTTTAAAT CAGACCTCGA GCAAGCTCAA AGTCTTCCAC CAGATGAAAT CGAGATCCTG 
TGCGGAGACG AGGCGGAAAA ACTCCCGGAA AGATCTTGCA GGATGGAGAG CGGAGAGGTG 
GATAACCGTA GAGCGGAGCA CAGCAGGACG CTTCCTGACC GGGTGGTCGC ACCGCTGCTG 
GCAGGGGGAC CAGAGGTCAG GCTTCGCAGG TCTGAGATGA AGTTGTGGGG TCCAGACGCA 
GATGGTGATG TGAAGGAGAA AAAGCGCAGC TCGTTGTTTT CACCCCGTAA AAGCAGGAAG 
AATGGAAATG CAGCAGCTGA GAGCGGGCGA GAGACAGGGA AGCACAAGTC CCTCTGGAAG 
ACTGTTTTTT CAGTGTATAA GAAGGATAAA AAGAGGAAGG AGGTGGCAGT GGTGGCGGAA 
ACACTTCCTG CTGCTAATAA TGAGAGCAAA CGGAAAGTGT CGGGAATCAA CAGAACCACA 
GACCTGTGTT TCCGGAAGAA TCCAAGTTTC TCTGAAGATA CGGATATGTC CTGTCATGCT 
CTTCTTGAAA GATGTCCTCT CCGAGCTCAG AGAGCAGGAA CAGAGGAGGA ACTCAATGCC 
AGGCTGACAC GTCGAGTGCA GAGAGCAGCG CGCAGACAGG CCAAGCAGGA GGAGCTGCGG 
AGGCTGCATA GAGCTCAGAT AATCCAGCGT CAGTTGGAGC AGGTGGAAGT GAAGCAGAGG 
CAGCTGGAGG AGAAAGGTGT GGCTGTGGAG AAAGCACTCA GAGGAGAAGC AGTTGAGCCT 
TTTGACGGTT CTCCCCGTAG AAGGCCTGTA TATCTCTGTC CTTGCTGTTC CTCTGAAGCC 
AAAGCTAAAG GAACCGAACA TGATCATTCG GTTTATGTCT GTACAGTCTT TTTCCTGTAC 
GTAGTGCAGG AGCCTCCACT TCACCAACTG AGAATGGGAA AGAAAGACGA TCCAAGCTTA 
ATGCATCAGT GGTTCAAACT GGTGCAGGAG AAGAATGCTC TCGTGCGTTA TGAATCTGAG 
CTCATGATAT TCGCTCGTGA ACTGGAGCTG GAGGACAGAC AGAGTCGCCT GCAGCAGGAG 
CTGAGAGAGC GCATGGCGGT GGACGATCAT CTAAAAGGAG AAGAGGAGCT GGCGGAGGAG 
AGGCGTATAT TGAGCGAGAT GCTGGATGTG GTGGAGCAGA GGGATGCACT GGTGGCCCTC 
CTGGAGGAGC AGAGAGTTCG AGAAAAAGAG GAGGACAGTG ACCTGGAGGC TGTGATGCTG 
TCTAAGGGCT TCAGTCTGCA TTGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329669.1
CDS	598..5727
Translation	MWDGQSEMCQAHVLFDSFVQAATCKETLRAFQDLCEELNLNPCGQPQFYHTLRSRLHYWKAKALWAKLDKRACQKEYMRGHACTSTTCLIIGAGPCGLRTAIELGFLGARVVLVEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRNVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTATRRQNYIHMYTGGVSSLAQQIANQIQSQQAQAPKLLHRRESGSQKEFPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSSESDLEEEELVWKKGEELHARTNGERKLDLEDELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNESKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEAVEPFDGSPRRRPVYLCPCCSSEAKAKGTEHDHSVYVCTVFFLYVVQEPPLHQLRMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	XM_021473994.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473994.1

ORF Insert Sequence:

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCTGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTAAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATTAATCGC AATACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AACGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTCGCTCTGG TGGGAGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGCTTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG CCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AACGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCACT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT ACTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCCGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC TTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTTGCTA AATTTGAAGA GAACTCGCCA TCGCCTTCAA CCACTGCAAC ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCAGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGAGTTTC CAGTAAATGT CGGCAGCAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTAAG CGCAGAGGGC AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGGCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGTTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGATGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAA TCTTATGAAG GATGCAGCGA CGACCCTGAT 
ATAGACGTCA GCTCACTCAG TGAGTCCAAG CTATGTGACC AACGGGAACA GGAGGAGTCG 
GTTCCTTTCC ACCAATCCCC TGCCTCTACA GAATCTCCAG TCTCAATGAA ACCTTCAGAA 
TCAGACCTCA CACCTGATCC GTCAACAACC CCCGAGAGCT CGCCTGCCAA GAGGTCAGAG 
GTGGTGGAGG AGTTCTGGAT GAAGAGTGCA GAGATCAGAA AGAGTTTAGG CCTGACTCCG 
CTTTCTAGAG AATTGCAGCC AAAACATATA GCAGCTTCAA CCCAGACATC TAATGTTAAA 
GAAAGCTTTT ACACCTCAGT CACATATAAC ACCGCTGCTT TGGATTCTCC AAACCAAAGT 
GCTAGAATCT GTGACTCCAG CACACAAACT CATTCTGTGA CAGATCTTCA AGAAACCTCC 
CCTCTTGGAC CTACAGATGG CGATGCTGGC GTCGATTTAG GACGTTGTTC TGTTGTTCAC 
AGACTCAGCA TCACTGTAGA AGGTTGTGTG ATGGCAGATA ACCAGGGTTT GGATTCTACC 
TCGTTTGCTA CTGAAAGTGT TTTAAACCCA GACGCAGGTC TTCCCACCCC TCCTTATAGT 
CCGTCTCACT CCCCTCTTGT TGGTAAACAG TGTCGTGCCC TTCACCATTC TGAGCCAATT 
CTAGACAGAG AGGTTATGGC TTTCTCATCT ACCTGCACTG CTTCTGTACC ACAGCGGAGC 
AGTTTTAAAT CAGACCTCGA GCAAGCTCAA AGTCTTCCAC CAGATGAAAT CGAGATCCTG 
TGCGGAGACG AGGCGGAAAA ACTCCCGGAA AGATCTTGCA GGATGGAGAG CGGAGAGGTG 
GATAACCGTA GAGCGGAGCA CAGCAGGACG CTTCCTGACC GGGTGGTCGC ACCGCTGCTG 
GCAGGGGGAC CAGAGGTCAG GCTTCGCAGG TCTGAGATGA AGTTGTGGGG TCCAGACGCA 
GATGGTGATG TGAAGGAGAA AAAGCGCAGC TCGTTGTTTT CACCCCGTAA AAGCAGGAAG 
AATGGAAATG CAGCAGCTGA GAGCGGGCGA GAGACAGGGA AGCACAAGTC CCTCTGGAAG 
ACTGTTTTTT CAGTGTATAA GAAGGATAAA AAGAGGAAGG AGGTGGCAGT GGTGGCGGAA 
ACACTTCCTG CTGCTAATAA TGAGAGCAAA CGGAAAGTGT CGGGAATCAA CAGAACCACA 
GACCTGTGTT TCCGGAAGAA TCCAAGTTTC TCTGAAGATA CGGATATGTC CTGTCATGCT 
CTTCTTGAAA GATGTCCTCT CCGAGCTCAG AGAGCAGGAA CAGAGGAGGA ACTCAATGCC 
AGGCTGACAC GTCGAGTGCA GAGAGCAGCG CGCAGACAGG CCAAGCAGGA GGAGCTGCGG 
AGGCTGCATA GAGCTCAGAT AATCCAGCGT CAGTTGGAGC AGGTGGAAGT GAAGCAGAGG 
CAGCTGGAGG AGAAAGGTGT GGCTGTGGAG AAAGCACTCA GAGGAGAAGC AGTTGAGCCT 
TTTGACGGTT CTCCCCGTAG AAGGCCTGTA TATCTCTGTC CTTGCTGTTC CTCTGAAGCC 
AAAGCTAAAG GAACCGAACA TGATCATTCG GTTTATGTCT GTACAGTCTT TTTCCTGTAC 
GTAGTGCAGG AGCCTCCACT TCACCAACTG AGAATGGGAA AGAAAGACGA TCCAAGCTTA 
ATGCATCAGT GGTTCAAACT GGTGCAGGAG AAGAATGCTC TCGTGCGTTA TGAATCTGAG 
CTCATGATAT TCGCTCGTGA ACTGGAGCTG GAGGACAGAC AGAGTCGCCT GCAGCAGGAG 
CTGAGAGAGC GCATGGCGGT GGACGATCAT CTAAAAGGAG AAGAGGAGCT GGCGGAGGAG 
AGGCGTATAT TGAGCGAGAT GCTGGATGTG GTGGAGCAGA GGGATGCACT GGTGGCCCTC 
CTGGAGGAGC AGAGAGTTCG AGAAAAAGAG GAGGACAGTG ACCTGGAGGC TGTGATGCTG 
TCTAAGGGCT TCAGTCTGCA TTGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( XM_021473995.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329670.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X4, mRNA.

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CloneID	ODa146496
Clone ID Related Accession (Same CDS sequence)	XM_021473995.1
Accession Version	XM_021473995.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395230.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCTGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTAAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATTAATCGC AATACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AACGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTCGCTCTGG TGGGAGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGCTTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG CCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AACGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCACT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT ACTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCCGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC TTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTTGCTA AATTTGAAGA GAACTCGCCA TCGCCTTCAA CCACTGCAAC ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCAGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGAGTTTC CAGTAAATGT CGGCAGCAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTAAG CGCAGAGGGC AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGGCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGTTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGATGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAG GGTGAGTACT GCCCTTGGGA GAGGGAACGA 
CTCTCAGGGG TGTGGCTTGA GGAAGAGGAG GCGGGGTTTG TTAAAGAATC TTATGAAGGA 
TGCAGCGACG ACCCTGATAT AGACGTCAGC TCACTCAGTG AGTCCAAGCT ATGTGACCAA 
CGGGAACAGG AGGAGTCGGT TCCTTTCCAC CAATCCCCTG CCTCTACAGA ATCTCCAGTC 
TCAATGAAAC CTTCAGAATC AGACCTCACA CCTGATCCGT CAACAACCCC CGAGAGCTCG 
CCTGCCAAGA GGTCAGAGGT GGTGGAGGAG TTCTGGATGA AGAGTGCAGA GATCAGAAAG 
AGTTTAGGCC TGACTCCGCT TTCTAGAGAA TTGCAGCCAA AACATATAGC AGCTTCAACC 
CAGACATCTA ATGTTAAAGA AAGCTTTTAC ACCTCAGTCA CATATAACAC CGCTGCTTTG 
GATTCTCCAA ACCAAAGTGC TAGAATCTGT GACTCCAGCA CACAAACTCA TTCTGTGACA 
GATCTTCAAG AAACCTCCCC TCTTGGACCT ACAGATGGCG ATGCTGGCGT CGATTTAGGA 
CGTTGTTCTG TTGTTCACAG ACTCAGCATC ACTGTAGAAG GTTGTGTGAT GGCAGATAAC 
CAGGGTTTGG ATTCTACCTC GTTTGCTACT GAAAGTGTTT TAAACCCAGA CGCAGGTCTT 
CCCACCCCTC CTTATAGTCC GTCTCACTCC CCTCTTGTTG GTAAACAGTG TCGTGCCCTT 
CACCATTCTG AGCCAATTCT AGACAGAGAG GTTATGGCTT TCTCATCTAC CTGCACTGCT 
TCTGTACCAC AGCGGAGCAG TTTTAAATCA GACCTCGAGC AAGCTCAAAG TCTTCCACCA 
GATGAAATCG AGATCCTGTG CGGAGACGAG GCGGAAAAAC TCCCGGAAAG ATCTTGCAGG 
ATGGAGAGCG GAGAGGTGGA TAACCGTAGA GCGGAGCACA GCAGGACGCT TCCTGACCGG 
GTGGTCGCAC CGCTGCTGGC AGGGGGACCA GAGGTCAGGC TTCGCAGGTC TGAGATGAAG 
TTGTGGGGTC CAGACGCAGA TGGTGATGTG AAGGAGAAAA AGCGCAGCTC GTTGTTTTCA 
CCCCGTAAAA GCAGGAAGAA TGGAAATGCA GCAGCTGAGA GCGGGCGAGA GACAGGGAAG 
CACAAGTCCC TCTGGAAGAC TGTTTTTTCA GTGTATAAGA AGGATAAAAA GAGGAAGGAG 
GTGGCAGTGG TGGCGGAAAC ACTTCCTGCT GCTAATAATG AGAGCAAACG GAAAGTGTCG 
GGAATCAACA GAACCACAGA CCTGTGTTTC CGGAAGAATC CAAGTTTCTC TGAAGATACG 
GATATGTCCT GTCATGCTCT TCTTGAAAGA TGTCCTCTCC GAGCTCAGAG AGCAGGAACA 
GAGGAGGAAC TCAATGCCAG GCTGACACGT CGAGTGCAGA GAGCAGCGCG CAGACAGGCC 
AAGCAGGAGG AGCTGCGGAG GCTGCATAGA GCTCAGATAA TCCAGCGTCA GTTGGAGCAG 
GTGGAAGTGA AGCAGAGGCA GCTGGAGGAG AAAGGTGTGG CTGTGGAGAA AGCACTCAGA 
GGAGAAGCAG ACTTCTGGGA AGACTCTAGT ACCTCTGTCC TCTTGGATGT ACATCTGTGT 
GGAATGGGAA AGAAAGACGA TCCAAGCTTA ATGCATCAGT GGTTCAAACT GGTGCAGGAG 
AAGAATGCTC TCGTGCGTTA TGAATCTGAG CTCATGATAT TCGCTCGTGA ACTGGAGCTG 
GAGGACAGAC AGAGTCGCCT GCAGCAGGAG CTGAGAGAGC GCATGGCGGT GGACGATCAT 
CTAAAAGGAG AAGAGGAGCT GGCGGAGGAG AGGCGTATAT TGAGCGAGAT GCTGGATGTG 
GTGGAGCAGA GGGATGCACT GGTGGCCCTC CTGGAGGAGC AGAGAGTTCG AGAAAAAGAG 
GAGGACAGTG ACCTGGAGGC TGTGATGCTG TCTAAGGGCT TCAGTCTGCA TTGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329670.1
CDS	598..5697
Translation	MWDGQSEMCQAHVLFDSFVQAATCKETLRAFQDLCEELNLNPCGQPQFYHTLRSRLHYWKAKALWAKLDKRACQKEYMRGHACTSTTCLIIGAGPCGLRTAIELGFLGARVVLVEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRNVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTATRRQNYIHMYTGGVSSLAQQIANQIQSQQAQAPKLLHRRESGSQKEFPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSSESDLEEEELVWKKGEELHARTNGERKLDLEDELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDEGEYCPWERERLSGVWLEEEEAGFVKESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNESKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEADFWEDSSTSVLLDVHLCGMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	XM_021473995.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473995.1

ORF Insert Sequence:

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCTGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTAAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATTAATCGC AATACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AACGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTCGCTCTGG TGGGAGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGCTTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG CCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AACGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCACT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT ACTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCCGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC TTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTTGCTA AATTTGAAGA GAACTCGCCA TCGCCTTCAA CCACTGCAAC ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCAGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGAGTTTC CAGTAAATGT CGGCAGCAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTAAG CGCAGAGGGC AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGGCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGTTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGATGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAG GGTGAGTACT GCCCTTGGGA GAGGGAACGA 
CTCTCAGGGG TGTGGCTTGA GGAAGAGGAG GCGGGGTTTG TTAAAGAATC TTATGAAGGA 
TGCAGCGACG ACCCTGATAT AGACGTCAGC TCACTCAGTG AGTCCAAGCT ATGTGACCAA 
CGGGAACAGG AGGAGTCGGT TCCTTTCCAC CAATCCCCTG CCTCTACAGA ATCTCCAGTC 
TCAATGAAAC CTTCAGAATC AGACCTCACA CCTGATCCGT CAACAACCCC CGAGAGCTCG 
CCTGCCAAGA GGTCAGAGGT GGTGGAGGAG TTCTGGATGA AGAGTGCAGA GATCAGAAAG 
AGTTTAGGCC TGACTCCGCT TTCTAGAGAA TTGCAGCCAA AACATATAGC AGCTTCAACC 
CAGACATCTA ATGTTAAAGA AAGCTTTTAC ACCTCAGTCA CATATAACAC CGCTGCTTTG 
GATTCTCCAA ACCAAAGTGC TAGAATCTGT GACTCCAGCA CACAAACTCA TTCTGTGACA 
GATCTTCAAG AAACCTCCCC TCTTGGACCT ACAGATGGCG ATGCTGGCGT CGATTTAGGA 
CGTTGTTCTG TTGTTCACAG ACTCAGCATC ACTGTAGAAG GTTGTGTGAT GGCAGATAAC 
CAGGGTTTGG ATTCTACCTC GTTTGCTACT GAAAGTGTTT TAAACCCAGA CGCAGGTCTT 
CCCACCCCTC CTTATAGTCC GTCTCACTCC CCTCTTGTTG GTAAACAGTG TCGTGCCCTT 
CACCATTCTG AGCCAATTCT AGACAGAGAG GTTATGGCTT TCTCATCTAC CTGCACTGCT 
TCTGTACCAC AGCGGAGCAG TTTTAAATCA GACCTCGAGC AAGCTCAAAG TCTTCCACCA 
GATGAAATCG AGATCCTGTG CGGAGACGAG GCGGAAAAAC TCCCGGAAAG ATCTTGCAGG 
ATGGAGAGCG GAGAGGTGGA TAACCGTAGA GCGGAGCACA GCAGGACGCT TCCTGACCGG 
GTGGTCGCAC CGCTGCTGGC AGGGGGACCA GAGGTCAGGC TTCGCAGGTC TGAGATGAAG 
TTGTGGGGTC CAGACGCAGA TGGTGATGTG AAGGAGAAAA AGCGCAGCTC GTTGTTTTCA 
CCCCGTAAAA GCAGGAAGAA TGGAAATGCA GCAGCTGAGA GCGGGCGAGA GACAGGGAAG 
CACAAGTCCC TCTGGAAGAC TGTTTTTTCA GTGTATAAGA AGGATAAAAA GAGGAAGGAG 
GTGGCAGTGG TGGCGGAAAC ACTTCCTGCT GCTAATAATG AGAGCAAACG GAAAGTGTCG 
GGAATCAACA GAACCACAGA CCTGTGTTTC CGGAAGAATC CAAGTTTCTC TGAAGATACG 
GATATGTCCT GTCATGCTCT TCTTGAAAGA TGTCCTCTCC GAGCTCAGAG AGCAGGAACA 
GAGGAGGAAC TCAATGCCAG GCTGACACGT CGAGTGCAGA GAGCAGCGCG CAGACAGGCC 
AAGCAGGAGG AGCTGCGGAG GCTGCATAGA GCTCAGATAA TCCAGCGTCA GTTGGAGCAG 
GTGGAAGTGA AGCAGAGGCA GCTGGAGGAG AAAGGTGTGG CTGTGGAGAA AGCACTCAGA 
GGAGAAGCAG ACTTCTGGGA AGACTCTAGT ACCTCTGTCC TCTTGGATGT ACATCTGTGT 
GGAATGGGAA AGAAAGACGA TCCAAGCTTA ATGCATCAGT GGTTCAAACT GGTGCAGGAG 
AAGAATGCTC TCGTGCGTTA TGAATCTGAG CTCATGATAT TCGCTCGTGA ACTGGAGCTG 
GAGGACAGAC AGAGTCGCCT GCAGCAGGAG CTGAGAGAGC GCATGGCGGT GGACGATCAT 
CTAAAAGGAG AAGAGGAGCT GGCGGAGGAG AGGCGTATAT TGAGCGAGAT GCTGGATGTG 
GTGGAGCAGA GGGATGCACT GGTGGCCCTC CTGGAGGAGC AGAGAGTTCG AGAAAAAGAG 
GAGGACAGTG ACCTGGAGGC TGTGATGCTG TCTAAGGGCT TCAGTCTGCA TTGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( XM_021473996.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329671.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X5, mRNA.

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CloneID	ODa146497
Clone ID Related Accession (Same CDS sequence)	XM_021473996.1
Accession Version	XM_021473996.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5097bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395230.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCTGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTAAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATTAATCGC AATACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AACGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTCGCTCTGG TGGGAGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGCTTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG CCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AACGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCACT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT ACTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCCGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC TTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTTGCTA AATTTGAAGA GAACTCGCCA TCGCCTTCAA CCACTGCAAC ACGCAGACAG 
GGTTCTCAGA AGGAGTTTCC AGTAAATGTC GGCAGCAGTG ATGTGTGTTA TTTCTGTGGC 
CGTCGCGTTT ATGTCATGGA GAGGCTAAGC GCAGAGGGCA AGTTCTTCCA CAGGAGCTGC 
TTCCAGTGCG ACCACTGCAG CTCCACGATA CGGCTCTCCA ACTACACTTA TGACCAGCTA 
CATGGCAAGT TTTACTGTAA GCATCACTTC AGCTTCAGAC TGGCAAGTGT GGCTCAGAGA 
AAGAGACCGG CCCCACCGGT GGCCCCTCGG CCTGCTCAGG CTTCCCTGGC TGCATCCTCG 
GCCTCTACCT CTTTGTCCTC TTTGGGTTCA GTGGGCACAG CCACACCAGA CAGCTGGTCC 
TCCAGCACCC ACACAGACAT GGCATCCAGC TTGGCCAAGA GACTGTGCGG CACACCAGAA 
CGCATTGAGC TTGAGAATTA CAAACCCTCC CCACAGAAAC AAGACAGCCC CCTGCAGGAG 
GTACCAGAGG AAACCCTCGC ACAGCACAAT CTCAGCGCTA GCCTGCAGGA GAAGAATGCT 
GAAGAGCAGT CCAGTAGCTC TGAGTCAGAT CTAGAGGAGG AGGAGCTTGT CTGGAAGAAG 
GGGGAGGAGC TACATGCCAG GACCAATGGA GAGAGGAAGC TTGACCTAGA GGATGAGCTG 
AAGGAAGAAG AGGGAGGGGA GAAGCTGGAA AAACAAGAGG GGGAGGAAGA AGGAGAAGTT 
TCAGAAGAAG AGCAAGACGA GGGAGATTCT AGTGATGAGG GTGAGTACTG CCCTTGGGAG 
AGGGAACGAC TCTCAGGGGT GTGGCTTGAG GAAGAGGAGG CGGGGTTTGT TAAAGAATCT 
TATGAAGGAT GCAGCGACGA CCCTGATATA GACGTCAGCT CACTCAGTGA GTCCAAGCTA 
TGTGACCAAC GGGAACAGGA GGAGTCGGTT CCTTTCCACC AATCCCCTGC CTCTACAGAA 
TCTCCAGTCT CAATGAAACC TTCAGAATCA GACCTCACAC CTGATCCGTC AACAACCCCC 
GAGAGCTCGC CTGCCAAGAG GTCAGAGGTG GTGGAGGAGT TCTGGATGAA GAGTGCAGAG 
ATCAGAAAGA GTTTAGGCCT GACTCCGCTT TCTAGAGAAT TGCAGCCAAA ACATATAGCA 
GCTTCAACCC AGACATCTAA TGTTAAAGAA AGCTTTTACA CCTCAGTCAC ATATAACACC 
GCTGCTTTGG ATTCTCCAAA CCAAAGTGCT AGAATCTGTG ACTCCAGCAC ACAAACTCAT 
TCTGTGACAG ATCTTCAAGA AACCTCCCCT CTTGGACCTA CAGATGGCGA TGCTGGCGTC 
GATTTAGGAC GTTGTTCTGT TGTTCACAGA CTCAGCATCA CTGTAGAAGG TTGTGTGATG 
GCAGATAACC AGGGTTTGGA TTCTACCTCG TTTGCTACTG AAAGTGTTTT AAACCCAGAC 
GCAGGTCTTC CCACCCCTCC TTATAGTCCG TCTCACTCCC CTCTTGTTGG TAAACAGTGT 
CGTGCCCTTC ACCATTCTGA GCCAATTCTA GACAGAGAGG TTATGGCTTT CTCATCTACC 
TGCACTGCTT CTGTACCACA GCGGAGCAGT TTTAAATCAG ACCTCGAGCA AGCTCAAAGT 
CTTCCACCAG ATGAAATCGA GATCCTGTGC GGAGACGAGG CGGAAAAACT CCCGGAAAGA 
TCTTGCAGGA TGGAGAGCGG AGAGGTGGAT AACCGTAGAG CGGAGCACAG CAGGACGCTT 
CCTGACCGGG TGGTCGCACC GCTGCTGGCA GGGGGACCAG AGGTCAGGCT TCGCAGGTCT 
GAGATGAAGT TGTGGGGTCC AGACGCAGAT GGTGATGTGA AGGAGAAAAA GCGCAGCTCG 
TTGTTTTCAC CCCGTAAAAG CAGGAAGAAT GGAAATGCAG CAGCTGAGAG CGGGCGAGAG 
ACAGGGAAGC ACAAGTCCCT CTGGAAGACT GTTTTTTCAG TGTATAAGAA GGATAAAAAG 
AGGAAGGAGG TGGCAGTGGT GGCGGAAACA CTTCCTGCTG CTAATAATGA GAGCAAACGG 
AAAGTGTCGG GAATCAACAG AACCACAGAC CTGTGTTTCC GGAAGAATCC AAGTTTCTCT 
GAAGATACGG ATATGTCCTG TCATGCTCTT CTTGAAAGAT GTCCTCTCCG AGCTCAGAGA 
GCAGGAACAG AGGAGGAACT CAATGCCAGG CTGACACGTC GAGTGCAGAG AGCAGCGCGC 
AGACAGGCCA AGCAGGAGGA GCTGCGGAGG CTGCATAGAG CTCAGATAAT CCAGCGTCAG 
TTGGAGCAGG TGGAAGTGAA GCAGAGGCAG CTGGAGGAGA AAGGTGTGGC TGTGGAGAAA 
GCACTCAGAG GAGAAGCAGT TGAGCCTTTT GACGGTTCTC CCCGTAGAAG GCCTGTATAT 
CTCTGTCCTT GCTGTTCCTC TGAAGCCAAA GCTAAAGGAA CCGAACATGA TCATTCGGTT 
TATGTCTGTA CAGTCTTTTT CCTGTACGTA GTGCAGGAGC CTCCACTTCA CCAACTGAGA 
ATGGGAAAGA AAGACGATCC AAGCTTAATG CATCAGTGGT TCAAACTGGT GCAGGAGAAG 
AATGCTCTCG TGCGTTATGA ATCTGAGCTC ATGATATTCG CTCGTGAACT GGAGCTGGAG 
GACAGACAGA GTCGCCTGCA GCAGGAGCTG AGAGAGCGCA TGGCGGTGGA CGATCATCTA 
AAAGGAGAAG AGGAGCTGGC GGAGGAGAGG CGTATATTGA GCGAGATGCT GGATGTGGTG 
GAGCAGAGGG ATGCACTGGT GGCCCTCCTG GAGGAGCAGA GAGTTCGAGA AAAAGAGGAG 
GACAGTGACC TGGAGGCTGT GATGCTGTCT AAGGGCTTCA GTCTGCATTG GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329671.1
CDS	598..5694
Translation	MWDGQSEMCQAHVLFDSFVQAATCKETLRAFQDLCEELNLNPCGQPQFYHTLRSRLHYWKAKALWAKLDKRACQKEYMRGHACTSTTCLIIGAGPCGLRTAIELGFLGARVVLVEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRNVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTATRRQGSQKEFPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSSESDLEEEELVWKKGEELHARTNGERKLDLEDELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDEGEYCPWERERLSGVWLEEEEAGFVKESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNESKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEAVEPFDGSPRRRPVYLCPCCSSEAKAKGTEHDHSVYVCTVFFLYVVQEPPLHQLRMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	XM_021473996.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473996.1

ORF Insert Sequence:

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCTGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTAAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATTAATCGC AATACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AACGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTCGCTCTGG TGGGAGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGCTTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG CCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AACGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCACT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT ACTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCCGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC TTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTTGCTA AATTTGAAGA GAACTCGCCA TCGCCTTCAA CCACTGCAAC ACGCAGACAG 
GGTTCTCAGA AGGAGTTTCC AGTAAATGTC GGCAGCAGTG ATGTGTGTTA TTTCTGTGGC 
CGTCGCGTTT ATGTCATGGA GAGGCTAAGC GCAGAGGGCA AGTTCTTCCA CAGGAGCTGC 
TTCCAGTGCG ACCACTGCAG CTCCACGATA CGGCTCTCCA ACTACACTTA TGACCAGCTA 
CATGGCAAGT TTTACTGTAA GCATCACTTC AGCTTCAGAC TGGCAAGTGT GGCTCAGAGA 
AAGAGACCGG CCCCACCGGT GGCCCCTCGG CCTGCTCAGG CTTCCCTGGC TGCATCCTCG 
GCCTCTACCT CTTTGTCCTC TTTGGGTTCA GTGGGCACAG CCACACCAGA CAGCTGGTCC 
TCCAGCACCC ACACAGACAT GGCATCCAGC TTGGCCAAGA GACTGTGCGG CACACCAGAA 
CGCATTGAGC TTGAGAATTA CAAACCCTCC CCACAGAAAC AAGACAGCCC CCTGCAGGAG 
GTACCAGAGG AAACCCTCGC ACAGCACAAT CTCAGCGCTA GCCTGCAGGA GAAGAATGCT 
GAAGAGCAGT CCAGTAGCTC TGAGTCAGAT CTAGAGGAGG AGGAGCTTGT CTGGAAGAAG 
GGGGAGGAGC TACATGCCAG GACCAATGGA GAGAGGAAGC TTGACCTAGA GGATGAGCTG 
AAGGAAGAAG AGGGAGGGGA GAAGCTGGAA AAACAAGAGG GGGAGGAAGA AGGAGAAGTT 
TCAGAAGAAG AGCAAGACGA GGGAGATTCT AGTGATGAGG GTGAGTACTG CCCTTGGGAG 
AGGGAACGAC TCTCAGGGGT GTGGCTTGAG GAAGAGGAGG CGGGGTTTGT TAAAGAATCT 
TATGAAGGAT GCAGCGACGA CCCTGATATA GACGTCAGCT CACTCAGTGA GTCCAAGCTA 
TGTGACCAAC GGGAACAGGA GGAGTCGGTT CCTTTCCACC AATCCCCTGC CTCTACAGAA 
TCTCCAGTCT CAATGAAACC TTCAGAATCA GACCTCACAC CTGATCCGTC AACAACCCCC 
GAGAGCTCGC CTGCCAAGAG GTCAGAGGTG GTGGAGGAGT TCTGGATGAA GAGTGCAGAG 
ATCAGAAAGA GTTTAGGCCT GACTCCGCTT TCTAGAGAAT TGCAGCCAAA ACATATAGCA 
GCTTCAACCC AGACATCTAA TGTTAAAGAA AGCTTTTACA CCTCAGTCAC ATATAACACC 
GCTGCTTTGG ATTCTCCAAA CCAAAGTGCT AGAATCTGTG ACTCCAGCAC ACAAACTCAT 
TCTGTGACAG ATCTTCAAGA AACCTCCCCT CTTGGACCTA CAGATGGCGA TGCTGGCGTC 
GATTTAGGAC GTTGTTCTGT TGTTCACAGA CTCAGCATCA CTGTAGAAGG TTGTGTGATG 
GCAGATAACC AGGGTTTGGA TTCTACCTCG TTTGCTACTG AAAGTGTTTT AAACCCAGAC 
GCAGGTCTTC CCACCCCTCC TTATAGTCCG TCTCACTCCC CTCTTGTTGG TAAACAGTGT 
CGTGCCCTTC ACCATTCTGA GCCAATTCTA GACAGAGAGG TTATGGCTTT CTCATCTACC 
TGCACTGCTT CTGTACCACA GCGGAGCAGT TTTAAATCAG ACCTCGAGCA AGCTCAAAGT 
CTTCCACCAG ATGAAATCGA GATCCTGTGC GGAGACGAGG CGGAAAAACT CCCGGAAAGA 
TCTTGCAGGA TGGAGAGCGG AGAGGTGGAT AACCGTAGAG CGGAGCACAG CAGGACGCTT 
CCTGACCGGG TGGTCGCACC GCTGCTGGCA GGGGGACCAG AGGTCAGGCT TCGCAGGTCT 
GAGATGAAGT TGTGGGGTCC AGACGCAGAT GGTGATGTGA AGGAGAAAAA GCGCAGCTCG 
TTGTTTTCAC CCCGTAAAAG CAGGAAGAAT GGAAATGCAG CAGCTGAGAG CGGGCGAGAG 
ACAGGGAAGC ACAAGTCCCT CTGGAAGACT GTTTTTTCAG TGTATAAGAA GGATAAAAAG 
AGGAAGGAGG TGGCAGTGGT GGCGGAAACA CTTCCTGCTG CTAATAATGA GAGCAAACGG 
AAAGTGTCGG GAATCAACAG AACCACAGAC CTGTGTTTCC GGAAGAATCC AAGTTTCTCT 
GAAGATACGG ATATGTCCTG TCATGCTCTT CTTGAAAGAT GTCCTCTCCG AGCTCAGAGA 
GCAGGAACAG AGGAGGAACT CAATGCCAGG CTGACACGTC GAGTGCAGAG AGCAGCGCGC 
AGACAGGCCA AGCAGGAGGA GCTGCGGAGG CTGCATAGAG CTCAGATAAT CCAGCGTCAG 
TTGGAGCAGG TGGAAGTGAA GCAGAGGCAG CTGGAGGAGA AAGGTGTGGC TGTGGAGAAA 
GCACTCAGAG GAGAAGCAGT TGAGCCTTTT GACGGTTCTC CCCGTAGAAG GCCTGTATAT 
CTCTGTCCTT GCTGTTCCTC TGAAGCCAAA GCTAAAGGAA CCGAACATGA TCATTCGGTT 
TATGTCTGTA CAGTCTTTTT CCTGTACGTA GTGCAGGAGC CTCCACTTCA CCAACTGAGA 
ATGGGAAAGA AAGACGATCC AAGCTTAATG CATCAGTGGT TCAAACTGGT GCAGGAGAAG 
AATGCTCTCG TGCGTTATGA ATCTGAGCTC ATGATATTCG CTCGTGAACT GGAGCTGGAG 
GACAGACAGA GTCGCCTGCA GCAGGAGCTG AGAGAGCGCA TGGCGGTGGA CGATCATCTA 
AAAGGAGAAG AGGAGCTGGC GGAGGAGAGG CGTATATTGA GCGAGATGCT GGATGTGGTG 
GAGCAGAGGG ATGCACTGGT GGCCCTCCTG GAGGAGCAGA GAGTTCGAGA AAAAGAGGAG 
GACAGTGACC TGGAGGCTGT GATGCTGTCT AAGGGCTTCA GTCTGCATTG GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( XM_021473997.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329672.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X6, mRNA.

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CloneID	ODa146498
Clone ID Related Accession (Same CDS sequence)	XM_021473997.1
Accession Version	XM_021473997.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395230.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCTGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTAAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATTAATCGC AATACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AACGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTCGCTCTGG TGGGAGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGCTTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG CCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AACGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCACT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT ACTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCCGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC TTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTTGCTA AATTTGAAGA GAACTCGCCA TCGCCTTCAA CCACTGCAAC ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCAGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGAGTTTC CAGTAAATGT CGGCAGCAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTAAG CGCAGAGGGC AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGGCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGTTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGATGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAG GGTGAGTACT GCCCTTGGGA GAGGGAACGA 
CTCTCAGGGG TGTGGCTTGA GGAAGAGGAG GCGGGGTTTG TTAAAGAATC TTATGAAGGA 
TGCAGCGACG ACCCTGATAT AGACGTCAGC TCACTCAGTG AGTCCAAGCT ATGTGACCAA 
CGGGAACAGG AGGAGTCGGT TCCTTTCCAC CAATCCCCTG CCTCTACAGA ATCTCCAGTC 
TCAATGAAAC CTTCAGAATC AGACCTCACA CCTGATCCGT CAACAACCCC CGAGAGCTCG 
CCTGCCAAGA GGTCAGAGGT GGTGGAGGAG TTCTGGATGA AGAGTGCAGA GATCAGAAAG 
AGTTTAGGCC TGACTCCGCT TTCTAGAGAA TTGCAGCCAA AACATATAGC AGCTTCAACC 
CAGACATCTA ATGTTAAAGA AAGCTTTTAC ACCTCAGTCA CATATAACAC CGCTGCTTTG 
GATTCTCCAA ACCAAAGTGC TAGAATCTGT GACTCCAGCA CACAAACTCA TTCTGTGACA 
GATCTTCAAG AAACCTCCCC TCTTGGACCT ACAGATGGCG ATGCTGGCGT CGATTTAGGA 
CGTTGTTCTG TTGTTCACAG ACTCAGCATC ACTGTAGAAG GTTGTGTGAT GGCAGATAAC 
CAGGGTTTGG ATTCTACCTC GTTTGCTACT GAAAGTGTTT TAAACCCAGA CGCAGGTCTT 
CCCACCCCTC CTTATAGTCC GTCTCACTCC CCTCTTGTTG GTAAACAGTG TCGTGCCCTT 
CACCATTCTG AGCCAATTCT AGACAGAGAG GTTATGGCTT TCTCATCTAC CTGCACTGCT 
TCTGTACCAC AGCGGAGCAG TTTTAAATCA GACCTCGAGC AAGCTCAAAG TCTTCCACCA 
GATGAAATCG AGATCCTGTG CGGAGACGAG GCGGAAAAAC TCCCGGAAAG ATCTTGCAGG 
ATGGAGAGCG GAGAGGTGGA TAACCGTAGA GCGGAGCACA GCAGGACGCT TCCTGACCGG 
GTGGTCGCAC CGCTGCTGGC AGGGGGACCA GAGGTCAGGC TTCGCAGGTC TGAGATGAAG 
TTGTGGGGTC CAGACGCAGA TGGTGATGTG AAGGAGAAAA AGCGCAGCTC GTTGTTTTCA 
CCCCGTAAAA GCAGGAAGAA TGGAAATGCA GCAGCTGAGA GCGGGCGAGA GACAGGGAAG 
CACAAGTCCC TCTGGAAGAC TGTTTTTTCA GTGTATAAGA AGGATAAAAA GAGGAAGGAG 
GTGGCAGTGG TGGCGGAAAC ACTTCCTGCT GCTAATAATG AGAGCAAACG GAAAGTGTCG 
GGAATCAACA GAACCACAGA CCTGTGTTTC CGGAAGAATC CAAGTTTCTC TGAAGATACG 
GATATGTCCT GTCATGCTCT TCTTGAAAGA TGTCCTCTCC GAGCTCAGAG AGCAGGAACA 
GAGGAGGAAC TCAATGCCAG GCTGACACGT CGAGTGCAGA GAGCAGCGCG CAGACAGGCC 
AAGCAGGAGG AGCTGCGGAG GCTGCATAGA GCTCAGATAA TCCAGCGTCA GTTGGAGCAG 
GTGGAAGTGA AGCAGAGGCA GCTGGAGGAG AAAGGTGTGG CTGTGGAGAA AGCACTCAGA 
GGAGAAGCAG GAATGGGAAA GAAAGACGAT CCAAGCTTAA TGCATCAGTG GTTCAAACTG 
GTGCAGGAGA AGAATGCTCT CGTGCGTTAT GAATCTGAGC TCATGATATT CGCTCGTGAA 
CTGGAGCTGG AGGACAGACA GAGTCGCCTG CAGCAGGAGC TGAGAGAGCG CATGGCGGTG 
GACGATCATC TAAAAGGAGA AGAGGAGCTG GCGGAGGAGA GGCGTATATT GAGCGAGATG 
CTGGATGTGG TGGAGCAGAG GGATGCACTG GTGGCCCTCC TGGAGGAGCA GAGAGTTCGA 
GAAAAAGAGG AGGACAGTGA CCTGGAGGCT GTGATGCTGT CTAAGGGCTT CAGTCTGCAT 
TGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329672.1
CDS	598..5646
Translation	MWDGQSEMCQAHVLFDSFVQAATCKETLRAFQDLCEELNLNPCGQPQFYHTLRSRLHYWKAKALWAKLDKRACQKEYMRGHACTSTTCLIIGAGPCGLRTAIELGFLGARVVLVEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRNVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTATRRQNYIHMYTGGVSSLAQQIANQIQSQQAQAPKLLHRRESGSQKEFPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSSESDLEEEELVWKKGEELHARTNGERKLDLEDELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDEGEYCPWERERLSGVWLEEEEAGFVKESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNESKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEAGMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	XM_021473997.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473997.1

ORF Insert Sequence:

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCTGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTAAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATTAATCGC AATACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AACGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTCGCTCTGG TGGGAGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGCTTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG CCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AACGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCACT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT ACTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCCGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC TTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTTGCTA AATTTGAAGA GAACTCGCCA TCGCCTTCAA CCACTGCAAC ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCAGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGAGTTTC CAGTAAATGT CGGCAGCAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTAAG CGCAGAGGGC AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGGCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGTTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGATGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAG GGTGAGTACT GCCCTTGGGA GAGGGAACGA 
CTCTCAGGGG TGTGGCTTGA GGAAGAGGAG GCGGGGTTTG TTAAAGAATC TTATGAAGGA 
TGCAGCGACG ACCCTGATAT AGACGTCAGC TCACTCAGTG AGTCCAAGCT ATGTGACCAA 
CGGGAACAGG AGGAGTCGGT TCCTTTCCAC CAATCCCCTG CCTCTACAGA ATCTCCAGTC 
TCAATGAAAC CTTCAGAATC AGACCTCACA CCTGATCCGT CAACAACCCC CGAGAGCTCG 
CCTGCCAAGA GGTCAGAGGT GGTGGAGGAG TTCTGGATGA AGAGTGCAGA GATCAGAAAG 
AGTTTAGGCC TGACTCCGCT TTCTAGAGAA TTGCAGCCAA AACATATAGC AGCTTCAACC 
CAGACATCTA ATGTTAAAGA AAGCTTTTAC ACCTCAGTCA CATATAACAC CGCTGCTTTG 
GATTCTCCAA ACCAAAGTGC TAGAATCTGT GACTCCAGCA CACAAACTCA TTCTGTGACA 
GATCTTCAAG AAACCTCCCC TCTTGGACCT ACAGATGGCG ATGCTGGCGT CGATTTAGGA 
CGTTGTTCTG TTGTTCACAG ACTCAGCATC ACTGTAGAAG GTTGTGTGAT GGCAGATAAC 
CAGGGTTTGG ATTCTACCTC GTTTGCTACT GAAAGTGTTT TAAACCCAGA CGCAGGTCTT 
CCCACCCCTC CTTATAGTCC GTCTCACTCC CCTCTTGTTG GTAAACAGTG TCGTGCCCTT 
CACCATTCTG AGCCAATTCT AGACAGAGAG GTTATGGCTT TCTCATCTAC CTGCACTGCT 
TCTGTACCAC AGCGGAGCAG TTTTAAATCA GACCTCGAGC AAGCTCAAAG TCTTCCACCA 
GATGAAATCG AGATCCTGTG CGGAGACGAG GCGGAAAAAC TCCCGGAAAG ATCTTGCAGG 
ATGGAGAGCG GAGAGGTGGA TAACCGTAGA GCGGAGCACA GCAGGACGCT TCCTGACCGG 
GTGGTCGCAC CGCTGCTGGC AGGGGGACCA GAGGTCAGGC TTCGCAGGTC TGAGATGAAG 
TTGTGGGGTC CAGACGCAGA TGGTGATGTG AAGGAGAAAA AGCGCAGCTC GTTGTTTTCA 
CCCCGTAAAA GCAGGAAGAA TGGAAATGCA GCAGCTGAGA GCGGGCGAGA GACAGGGAAG 
CACAAGTCCC TCTGGAAGAC TGTTTTTTCA GTGTATAAGA AGGATAAAAA GAGGAAGGAG 
GTGGCAGTGG TGGCGGAAAC ACTTCCTGCT GCTAATAATG AGAGCAAACG GAAAGTGTCG 
GGAATCAACA GAACCACAGA CCTGTGTTTC CGGAAGAATC CAAGTTTCTC TGAAGATACG 
GATATGTCCT GTCATGCTCT TCTTGAAAGA TGTCCTCTCC GAGCTCAGAG AGCAGGAACA 
GAGGAGGAAC TCAATGCCAG GCTGACACGT CGAGTGCAGA GAGCAGCGCG CAGACAGGCC 
AAGCAGGAGG AGCTGCGGAG GCTGCATAGA GCTCAGATAA TCCAGCGTCA GTTGGAGCAG 
GTGGAAGTGA AGCAGAGGCA GCTGGAGGAG AAAGGTGTGG CTGTGGAGAA AGCACTCAGA 
GGAGAAGCAG GAATGGGAAA GAAAGACGAT CCAAGCTTAA TGCATCAGTG GTTCAAACTG 
GTGCAGGAGA AGAATGCTCT CGTGCGTTAT GAATCTGAGC TCATGATATT CGCTCGTGAA 
CTGGAGCTGG AGGACAGACA GAGTCGCCTG CAGCAGGAGC TGAGAGAGCG CATGGCGGTG 
GACGATCATC TAAAAGGAGA AGAGGAGCTG GCGGAGGAGA GGCGTATATT GAGCGAGATG 
CTGGATGTGG TGGAGCAGAG GGATGCACTG GTGGCCCTCC TGGAGGAGCA GAGAGTTCGA 
GAAAAAGAGG AGGACAGTGA CCTGGAGGCT GTGATGCTGT CTAAGGGCTT CAGTCTGCAT 
TGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( XM_021473998.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329673.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X7, mRNA.

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CloneID	ODa146499
Clone ID Related Accession (Same CDS sequence)	XM_021473998.1
Accession Version	XM_021473998.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395230.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTTAA AGGTGGCCTT GTTATTGGGT GTCGAAGTTC ATGTGAACGT GGAGTTCAAA 
CATCTACTGG AACCTCCAGA GAACCAGGAG AAACGTGTTG GTTGGCGGGC AGAGGTTCAG 
CCCAGCTCTC ATCCAGTAAG ACAGCTGGAG TTTGATGTGG TTATTGGGGC AGACGGCAGA 
AGAAACACAT TACCAGGGTT TCGCAGGAAG GAGTTTCGTG GGAAACTGGC CATTGCCATC 
ACGGCGAATT TCATTAATCG CAATACCACC GCCGAGGCTA AAGTGGAGGA GATCAGCGGC 
GTCGCCTTCA TTTTCAACCA GAGGTTTTTC CAGGACCTCC GGCAAGCTAC CGGAATCGAT 
CTGGAAAATA TAGTATACTA TAAAGACGAC ACTCACTATT TCGTCATGAC CGCCAAAAAA 
CAAAGTCTTC TGGAGAAAGG AGTCATCCTG CGTGATTATG CTGACACTGA GATGCTGCTG 
TCCAGACATA ATGTGGACCA GAACGCACTG CTCTCGTATG CACGTGAAGC GGCTGATTTC 
TCCACCAATC ATCAGCTTCC AGCGCTGGAT TTTGCCATCA ATCACTATGG GCAGTCAGAT 
GTGGCCATGT TTGACTTTAC CTGTATGTAC GCCTCGGAAA ACGCAGCGAT GGTACGTCAG 
CGTATGGGAC ATCCACTGCT CGTCGCTCTG GTGGGAGACA GTTTGCTGGA GCCGTTCTGG 
CCAATGGGAA CAGGGATAGC TCGGGGCTTC CTGGCAGCGA TGGACACAGC GTGGATGGTG 
CGCAGCTGGG GTCAGGGCAA CACTCCTCTG GAGGTTCTGG CTGAGAGAGA GAGTGTGTAT 
AGGTTGCTTC CTCAGACAAC TCCAGAAAAT GTCAGTAAAA ACTACAGCCA GTTCAGTGTG 
GATCCTGCTA GCCGCTATCC CAACATTAAT ATGCAGCTCA TCAACGCCGC TCAGGTGCGT 
CACCTCATAG ACACAGGTGA GGGTCCAGTT TTGGGTCTCG ATGCTGTTAG TTCTCCACAC 
CCAAGACTTA CGCGACAAGA GTCAATGGCT CGGTACAGTA AACTGCTGAG CTGGTGTCAG 
GAACACACTC ATGGTTACAG GAACGTGTGT GTGACGGACT TCACCTCTTC CTGGAGGAGC 
GGACTGGCAC TGTGTGCTCT CATTCACACC TTCAGGCCTG ACCTCATTGA TTTTGCATCT 
CTGGAGGAGA GCGAGGCAGA GTTCAGTGGT CAGCTGGGTT TGGACGTGGC GGAGCAGGAA 
TTTGGTATTT GTCCTATAAT GACCGGCAAG GAGATGAGTG TACTGGAAGA GAGCGACTCT 
CTCTGTATGG TCATGTACCT CAGTCAGCTT CATGAGCTCC TCAAAGACAC ATCGCCACCT 
AGTGGAAGTC AGAGCTCAGA AGAGAGAGCG GTTTTGTTCT CCAGCAGCAG ATCACCAATC 
TCACTGCTCA GCAAACTGGG ACAGAGTTTG TCCCGCAAAC GCAATCCTAA GGATAAGAAA 
GAGAAGGAAG CAGACAGTGT TGGAAAGAGG AGGAGAACAA GTCAGGCCGG CCAATCCGAA 
GAGGAGGATG CTCTTCATGA CGGCAATGAA AATAAGTCTC CAGCAGAAAC TCCCGGCTCA 
GAACCGAAGG CTTCTGAGGG TCACAGCAAG GTTCGGTCCA TGGCGTCGCT CCTTCTTGCT 
AAATTTGAAG AGAACTCGCC ATCGCCTTCA ACCACTGCAA CACGCAGACA GAACTACATT 
CACATGTATA CCGGCGGAGT GAGCTCATTG GCTCAGCAGA TAGCCAATCA GATTCAAAGT 
CAGCAGGCTC AGGCTCCCAA GCTCCTTCAC AGGAGGGAGT CGGGTTCTCA GAAGGAGTTT 
CCAGTAAATG TCGGCAGCAG TGATGTGTGT TATTTCTGTG GCCGTCGCGT TTATGTCATG 
GAGAGGCTAA GCGCAGAGGG CAAGTTCTTC CACAGGAGCT GCTTCCAGTG CGACCACTGC 
AGCTCCACGA TACGGCTCTC CAACTACACT TATGACCAGC TACATGGCAA GTTTTACTGT 
AAGCATCACT TCAGCTTCAG ACTGGCAAGT GTGGCTCAGA GAAAGAGACC GGCCCCACCG 
GTGGCCCCTC GGCCTGCTCA GGCTTCCCTG GCTGCATCCT CGGCCTCTAC CTCTTTGTCC 
TCTTTGGGTT CAGTGGGCAC AGCCACACCA GACAGCTGGT CCTCCAGCAC CCACACAGAC 
ATGGCATCCA GCTTGGCCAA GAGACTGTGC GGCACACCAG AACGCATTGA GCTTGAGAAT 
TACAAACCCT CCCCACAGAA ACAAGACAGC CCCCTGCAGG AGGTACCAGA GGAAACCCTC 
GCACAGCACA ATCTCAGCGC TAGCCTGCAG GAGAAGAATG CTGAAGAGCA GTCCAGTAGC 
TCTGAGTCAG ATCTAGAGGA GGAGGAGCTT GTCTGGAAGA AGGGGGAGGA GCTACATGCC 
AGGACCAATG GAGAGAGGAA GCTTGACCTA GAGGATGAGC TGAAGGAAGA AGAGGGAGGG 
GAGAAGCTGG AAAAACAAGA GGGGGAGGAA GAAGGAGAAG TTTCAGAAGA AGAGCAAGAC 
GAGGGAGATT CTAGTGATGA GGGTGAGTAC TGCCCTTGGG AGAGGGAACG ACTCTCAGGG 
GTGTGGCTTG AGGAAGAGGA GGCGGGGTTT GTTAAAGAAT CTTATGAAGG ATGCAGCGAC 
GACCCTGATA TAGACGTCAG CTCACTCAGT GAGTCCAAGC TATGTGACCA ACGGGAACAG 
GAGGAGTCGG TTCCTTTCCA CCAATCCCCT GCCTCTACAG AATCTCCAGT CTCAATGAAA 
CCTTCAGAAT CAGACCTCAC ACCTGATCCG TCAACAACCC CCGAGAGCTC GCCTGCCAAG 
AGGTCAGAGG TGGTGGAGGA GTTCTGGATG AAGAGTGCAG AGATCAGAAA GAGTTTAGGC 
CTGACTCCGC TTTCTAGAGA ATTGCAGCCA AAACATATAG CAGCTTCAAC CCAGACATCT 
AATGTTAAAG AAAGCTTTTA CACCTCAGTC ACATATAACA CCGCTGCTTT GGATTCTCCA 
AACCAAAGTG CTAGAATCTG TGACTCCAGC ACACAAACTC ATTCTGTGAC AGATCTTCAA 
GAAACCTCCC CTCTTGGACC TACAGATGGC GATGCTGGCG TCGATTTAGG ACGTTGTTCT 
GTTGTTCACA GACTCAGCAT CACTGTAGAA GGTTGTGTGA TGGCAGATAA CCAGGGTTTG 
GATTCTACCT CGTTTGCTAC TGAAAGTGTT TTAAACCCAG ACGCAGGTCT TCCCACCCCT 
CCTTATAGTC CGTCTCACTC CCCTCTTGTT GGTAAACAGT GTCGTGCCCT TCACCATTCT 
GAGCCAATTC TAGACAGAGA GGTTATGGCT TTCTCATCTA CCTGCACTGC TTCTGTACCA 
CAGCGGAGCA GTTTTAAATC AGACCTCGAG CAAGCTCAAA GTCTTCCACC AGATGAAATC 
GAGATCCTGT GCGGAGACGA GGCGGAAAAA CTCCCGGAAA GATCTTGCAG GATGGAGAGC 
GGAGAGGTGG ATAACCGTAG AGCGGAGCAC AGCAGGACGC TTCCTGACCG GGTGGTCGCA 
CCGCTGCTGG CAGGGGGACC AGAGGTCAGG CTTCGCAGGT CTGAGATGAA GTTGTGGGGT 
CCAGACGCAG ATGGTGATGT GAAGGAGAAA AAGCGCAGCT CGTTGTTTTC ACCCCGTAAA 
AGCAGGAAGA ATGGAAATGC AGCAGCTGAG AGCGGGCGAG AGACAGGGAA GCACAAGTCC 
CTCTGGAAGA CTGTTTTTTC AGTGTATAAG AAGGATAAAA AGAGGAAGGA GGTGGCAGTG 
GTGGCGGAAA CACTTCCTGC TGCTAATAAT GAGAGCAAAC GGAAAGTGTC GGGAATCAAC 
AGAACCACAG ACCTGTGTTT CCGGAAGAAT CCAAGTTTCT CTGAAGATAC GGATATGTCC 
TGTCATGCTC TTCTTGAAAG ATGTCCTCTC CGAGCTCAGA GAGCAGGAAC AGAGGAGGAA 
CTCAATGCCA GGCTGACACG TCGAGTGCAG AGAGCAGCGC GCAGACAGGC CAAGCAGGAG 
GAGCTGCGGA GGCTGCATAG AGCTCAGATA ATCCAGCGTC AGTTGGAGCA GGTGGAAGTG 
AAGCAGAGGC AGCTGGAGGA GAAAGGTGTG GCTGTGGAGA AAGCACTCAG AGGAGAAGCA 
GTTGAGCCTT TTGACGGTTC TCCCCGTAGA AGGCCTGTAT ATCTCTGTCC TTGCTGTTCC 
TCTGAAGCCA AAGCTAAAGG AACCGAACAT GATCATTCGG TTTATGTCTG TACAGTCTTT 
TTCCTGTACG TAGTGCAGGA GCCTCCACTT CACCAACTGA GAATGGGAAA GAAAGACGAT 
CCAAGCTTAA TGCATCAGTG GTTCAAACTG GTGCAGGAGA AGAATGCTCT CGTGCGTTAT 
GAATCTGAGC TCATGATATT CGCTCGTGAA CTGGAGCTGG AGGACAGACA GAGTCGCCTG 
CAGCAGGAGC TGAGAGAGCG CATGGCGGTG GACGATCATC TAAAAGGAGA AGAGGAGCTG 
GCGGAGGAGA GGCGTATATT GAGCGAGATG CTGGATGTGG TGGAGCAGAG GGATGCACTG 
GTGGCCCTCC TGGAGGAGCA GAGAGTTCGA GAAAAAGAGG AGGACAGTGA CCTGGAGGCT 
GTGATGCTGT CTAAGGGCTT CAGTCTGCAT TGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329673.1
CDS	86..4804
Translation	MLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRNVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTATRRQNYIHMYTGGVSSLAQQIANQIQSQQAQAPKLLHRRESGSQKEFPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSSESDLEEEELVWKKGEELHARTNGERKLDLEDELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDEGEYCPWERERLSGVWLEEEEAGFVKESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNESKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEAVEPFDGSPRRRPVYLCPCCSSEAKAKGTEHDHSVYVCTVFFLYVVQEPPLHQLRMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	XM_021473998.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473998.1

ORF Insert Sequence:

ATGCTTTTAA AGGTGGCCTT GTTATTGGGT GTCGAAGTTC ATGTGAACGT GGAGTTCAAA 
CATCTACTGG AACCTCCAGA GAACCAGGAG AAACGTGTTG GTTGGCGGGC AGAGGTTCAG 
CCCAGCTCTC ATCCAGTAAG ACAGCTGGAG TTTGATGTGG TTATTGGGGC AGACGGCAGA 
AGAAACACAT TACCAGGGTT TCGCAGGAAG GAGTTTCGTG GGAAACTGGC CATTGCCATC 
ACGGCGAATT TCATTAATCG CAATACCACC GCCGAGGCTA AAGTGGAGGA GATCAGCGGC 
GTCGCCTTCA TTTTCAACCA GAGGTTTTTC CAGGACCTCC GGCAAGCTAC CGGAATCGAT 
CTGGAAAATA TAGTATACTA TAAAGACGAC ACTCACTATT TCGTCATGAC CGCCAAAAAA 
CAAAGTCTTC TGGAGAAAGG AGTCATCCTG CGTGATTATG CTGACACTGA GATGCTGCTG 
TCCAGACATA ATGTGGACCA GAACGCACTG CTCTCGTATG CACGTGAAGC GGCTGATTTC 
TCCACCAATC ATCAGCTTCC AGCGCTGGAT TTTGCCATCA ATCACTATGG GCAGTCAGAT 
GTGGCCATGT TTGACTTTAC CTGTATGTAC GCCTCGGAAA ACGCAGCGAT GGTACGTCAG 
CGTATGGGAC ATCCACTGCT CGTCGCTCTG GTGGGAGACA GTTTGCTGGA GCCGTTCTGG 
CCAATGGGAA CAGGGATAGC TCGGGGCTTC CTGGCAGCGA TGGACACAGC GTGGATGGTG 
CGCAGCTGGG GTCAGGGCAA CACTCCTCTG GAGGTTCTGG CTGAGAGAGA GAGTGTGTAT 
AGGTTGCTTC CTCAGACAAC TCCAGAAAAT GTCAGTAAAA ACTACAGCCA GTTCAGTGTG 
GATCCTGCTA GCCGCTATCC CAACATTAAT ATGCAGCTCA TCAACGCCGC TCAGGTGCGT 
CACCTCATAG ACACAGGTGA GGGTCCAGTT TTGGGTCTCG ATGCTGTTAG TTCTCCACAC 
CCAAGACTTA CGCGACAAGA GTCAATGGCT CGGTACAGTA AACTGCTGAG CTGGTGTCAG 
GAACACACTC ATGGTTACAG GAACGTGTGT GTGACGGACT TCACCTCTTC CTGGAGGAGC 
GGACTGGCAC TGTGTGCTCT CATTCACACC TTCAGGCCTG ACCTCATTGA TTTTGCATCT 
CTGGAGGAGA GCGAGGCAGA GTTCAGTGGT CAGCTGGGTT TGGACGTGGC GGAGCAGGAA 
TTTGGTATTT GTCCTATAAT GACCGGCAAG GAGATGAGTG TACTGGAAGA GAGCGACTCT 
CTCTGTATGG TCATGTACCT CAGTCAGCTT CATGAGCTCC TCAAAGACAC ATCGCCACCT 
AGTGGAAGTC AGAGCTCAGA AGAGAGAGCG GTTTTGTTCT CCAGCAGCAG ATCACCAATC 
TCACTGCTCA GCAAACTGGG ACAGAGTTTG TCCCGCAAAC GCAATCCTAA GGATAAGAAA 
GAGAAGGAAG CAGACAGTGT TGGAAAGAGG AGGAGAACAA GTCAGGCCGG CCAATCCGAA 
GAGGAGGATG CTCTTCATGA CGGCAATGAA AATAAGTCTC CAGCAGAAAC TCCCGGCTCA 
GAACCGAAGG CTTCTGAGGG TCACAGCAAG GTTCGGTCCA TGGCGTCGCT CCTTCTTGCT 
AAATTTGAAG AGAACTCGCC ATCGCCTTCA ACCACTGCAA CACGCAGACA GAACTACATT 
CACATGTATA CCGGCGGAGT GAGCTCATTG GCTCAGCAGA TAGCCAATCA GATTCAAAGT 
CAGCAGGCTC AGGCTCCCAA GCTCCTTCAC AGGAGGGAGT CGGGTTCTCA GAAGGAGTTT 
CCAGTAAATG TCGGCAGCAG TGATGTGTGT TATTTCTGTG GCCGTCGCGT TTATGTCATG 
GAGAGGCTAA GCGCAGAGGG CAAGTTCTTC CACAGGAGCT GCTTCCAGTG CGACCACTGC 
AGCTCCACGA TACGGCTCTC CAACTACACT TATGACCAGC TACATGGCAA GTTTTACTGT 
AAGCATCACT TCAGCTTCAG ACTGGCAAGT GTGGCTCAGA GAAAGAGACC GGCCCCACCG 
GTGGCCCCTC GGCCTGCTCA GGCTTCCCTG GCTGCATCCT CGGCCTCTAC CTCTTTGTCC 
TCTTTGGGTT CAGTGGGCAC AGCCACACCA GACAGCTGGT CCTCCAGCAC CCACACAGAC 
ATGGCATCCA GCTTGGCCAA GAGACTGTGC GGCACACCAG AACGCATTGA GCTTGAGAAT 
TACAAACCCT CCCCACAGAA ACAAGACAGC CCCCTGCAGG AGGTACCAGA GGAAACCCTC 
GCACAGCACA ATCTCAGCGC TAGCCTGCAG GAGAAGAATG CTGAAGAGCA GTCCAGTAGC 
TCTGAGTCAG ATCTAGAGGA GGAGGAGCTT GTCTGGAAGA AGGGGGAGGA GCTACATGCC 
AGGACCAATG GAGAGAGGAA GCTTGACCTA GAGGATGAGC TGAAGGAAGA AGAGGGAGGG 
GAGAAGCTGG AAAAACAAGA GGGGGAGGAA GAAGGAGAAG TTTCAGAAGA AGAGCAAGAC 
GAGGGAGATT CTAGTGATGA GGGTGAGTAC TGCCCTTGGG AGAGGGAACG ACTCTCAGGG 
GTGTGGCTTG AGGAAGAGGA GGCGGGGTTT GTTAAAGAAT CTTATGAAGG ATGCAGCGAC 
GACCCTGATA TAGACGTCAG CTCACTCAGT GAGTCCAAGC TATGTGACCA ACGGGAACAG 
GAGGAGTCGG TTCCTTTCCA CCAATCCCCT GCCTCTACAG AATCTCCAGT CTCAATGAAA 
CCTTCAGAAT CAGACCTCAC ACCTGATCCG TCAACAACCC CCGAGAGCTC GCCTGCCAAG 
AGGTCAGAGG TGGTGGAGGA GTTCTGGATG AAGAGTGCAG AGATCAGAAA GAGTTTAGGC 
CTGACTCCGC TTTCTAGAGA ATTGCAGCCA AAACATATAG CAGCTTCAAC CCAGACATCT 
AATGTTAAAG AAAGCTTTTA CACCTCAGTC ACATATAACA CCGCTGCTTT GGATTCTCCA 
AACCAAAGTG CTAGAATCTG TGACTCCAGC ACACAAACTC ATTCTGTGAC AGATCTTCAA 
GAAACCTCCC CTCTTGGACC TACAGATGGC GATGCTGGCG TCGATTTAGG ACGTTGTTCT 
GTTGTTCACA GACTCAGCAT CACTGTAGAA GGTTGTGTGA TGGCAGATAA CCAGGGTTTG 
GATTCTACCT CGTTTGCTAC TGAAAGTGTT TTAAACCCAG ACGCAGGTCT TCCCACCCCT 
CCTTATAGTC CGTCTCACTC CCCTCTTGTT GGTAAACAGT GTCGTGCCCT TCACCATTCT 
GAGCCAATTC TAGACAGAGA GGTTATGGCT TTCTCATCTA CCTGCACTGC TTCTGTACCA 
CAGCGGAGCA GTTTTAAATC AGACCTCGAG CAAGCTCAAA GTCTTCCACC AGATGAAATC 
GAGATCCTGT GCGGAGACGA GGCGGAAAAA CTCCCGGAAA GATCTTGCAG GATGGAGAGC 
GGAGAGGTGG ATAACCGTAG AGCGGAGCAC AGCAGGACGC TTCCTGACCG GGTGGTCGCA 
CCGCTGCTGG CAGGGGGACC AGAGGTCAGG CTTCGCAGGT CTGAGATGAA GTTGTGGGGT 
CCAGACGCAG ATGGTGATGT GAAGGAGAAA AAGCGCAGCT CGTTGTTTTC ACCCCGTAAA 
AGCAGGAAGA ATGGAAATGC AGCAGCTGAG AGCGGGCGAG AGACAGGGAA GCACAAGTCC 
CTCTGGAAGA CTGTTTTTTC AGTGTATAAG AAGGATAAAA AGAGGAAGGA GGTGGCAGTG 
GTGGCGGAAA CACTTCCTGC TGCTAATAAT GAGAGCAAAC GGAAAGTGTC GGGAATCAAC 
AGAACCACAG ACCTGTGTTT CCGGAAGAAT CCAAGTTTCT CTGAAGATAC GGATATGTCC 
TGTCATGCTC TTCTTGAAAG ATGTCCTCTC CGAGCTCAGA GAGCAGGAAC AGAGGAGGAA 
CTCAATGCCA GGCTGACACG TCGAGTGCAG AGAGCAGCGC GCAGACAGGC CAAGCAGGAG 
GAGCTGCGGA GGCTGCATAG AGCTCAGATA ATCCAGCGTC AGTTGGAGCA GGTGGAAGTG 
AAGCAGAGGC AGCTGGAGGA GAAAGGTGTG GCTGTGGAGA AAGCACTCAG AGGAGAAGCA 
GTTGAGCCTT TTGACGGTTC TCCCCGTAGA AGGCCTGTAT ATCTCTGTCC TTGCTGTTCC 
TCTGAAGCCA AAGCTAAAGG AACCGAACAT GATCATTCGG TTTATGTCTG TACAGTCTTT 
TTCCTGTACG TAGTGCAGGA GCCTCCACTT CACCAACTGA GAATGGGAAA GAAAGACGAT 
CCAAGCTTAA TGCATCAGTG GTTCAAACTG GTGCAGGAGA AGAATGCTCT CGTGCGTTAT 
GAATCTGAGC TCATGATATT CGCTCGTGAA CTGGAGCTGG AGGACAGACA GAGTCGCCTG 
CAGCAGGAGC TGAGAGAGCG CATGGCGGTG GACGATCATC TAAAAGGAGA AGAGGAGCTG 
GCGGAGGAGA GGCGTATATT GAGCGAGATG CTGGATGTGG TGGAGCAGAG GGATGCACTG 
GTGGCCCTCC TGGAGGAGCA GAGAGTTCGA GAAAAAGAGG AGGACAGTGA CCTGGAGGCT 
GTGATGCTGT CTAAGGGCTT CAGTCTGCAT TGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( XM_009300192.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298467.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa44975
Clone ID Related Accession (Same CDS sequence)	XM_009300192.2 , XM_009300192.3
Accession Version	XM_009300192.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300192.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGAGGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAG GGTGAGTACT GCCCTTGGGA GAGGGAACGA 
CTCTCAGGGG TGTGGCTTGA GGAAGAGGAG GCGGGGTTTG TTAAAGAATC TTATGAAGGA 
TGCAGCGACG ACCCTGATAT AGACGTCAGC TCACTCAGTG AGTCCAAGCT ATGTGACCAA 
CGGGAACAGG AGGAGTCTGT TCCTTTCCAC CAATCCCCTG CCTCTACAGA ATCTCCAGTC 
TCAATGAAAC CTTCAGAATC AGACCTCACA CCTGATCCGT CAACAACCCC CGAGAGCTCG 
CCTGCCAAGA GGTCAGAGGT GGTGGAGGAG TTCTGGATGA AGAGTGCAGA GATCCGAAAG 
AGTTTAGGCC TGACTCCGCT TTCTAGAGAA TTGCAGCCAA AACATATAGC AGCTTCAACC 
CAGACATCTA ATGTTAAAGA AAGCTTTTAC ACCTCAGTCA CATATAACAC CGCTGCTTTG 
GATTCTCCAA ACCAAAGTGC TAGAATCTGT GACTCCAGCA CACAAACTCA TTCTGTGACA 
GATCTTCAAG AAACCTCCCC TCTTGGACCT ACAGATGGCG ATGCTGGCGT CGATTTAGGA 
CGTTGTTCTG TTGTTCACAG ACTCAGCATC ACTGTAGAAG GTTGTGTGAT GGCAGATAAC 
CAGGGTTTGG ATTCTACCTC GTTTGCTACT GAAAGTGTTT TAAACCCAGA CGCAGGTCTT 
CCCACCCCTC CTTATAGTCC GTCTCACTCC CCTCTTGTTG GTAAACAGTG TCGTGCCCTT 
CACCATTCTG AGCCAATTCT AGACAGAGAG GTTATGGCTT TCTCATCTAC CTGCACTGCT 
TCTGTACCAC AGCGGAGCAG TTTTAAATCA GACCTCGAGC AAGCTCAAAG TCTTCCACCA 
GATGAAATCG AGATCCTGTG CGGAGACGAG GCGGAAAAAC TCCCGGAAAG ATCTTGCAGG 
ATGGAGAGCG GAGAGGTGGA TAACCGTAGA GCGGAGCACA GCAGGACGCT TCCTGACCGG 
GTGGTCGCAC CGCTGCTGGC AGGGGGACCA GAGGTCAGGC TTCGCAGGTC TGAGATGAAG 
TTGTGGGGTC CAGACGCAGA TGGTGATGTG AAGGAGAAAA AGCGCAGCTC GTTGTTTTCA 
CCCCGTAAAA GCAGGAAGAA TGGAAATGCA GCAGCTGAGA GCGGGCGAGA GACAGGGAAG 
CACAAGTCCC TCTGGAAGAC TGTTTTTTCA GTGTATAAGA AGGATAAAAA GAGGAAGGAG 
GTGGCAGTGG TGGCGGAAAC ACTTCCTGCT GCTAATAATG GGAGCAAACG GAAAGTGTCG 
GGAATTAACA GAACCACAGA CCTGTGTTTC CGGAAGAATC CAAGTTTCTC TGAAGATACG 
GATATGTCCT GTCATGCTCT TCTTGAAAGA TGTCCTCTCC GAGCTCAGAG AGCAGGAACA 
GAGGAGGAAC TCAATGCCAG GCTGACACGT CGAGTGCAGA GAGCAGCGCG CAGACAGGCC 
AAGCAGGAGG AGCTGCGGAG GCTGCATAGA GCTCAGATAA TCCAGCGTCA GTTGGAGCAG 
GTGGAAGTGA AGCAGAGGCA GCTGGAGGAG AAAGGTGTGG CTGTGGAGAA AGCACTCAGA 
GGAGAAGCAG ACTTCTGGGA AGACTCTAGT ACCTCTGTCC TCTTGGATGT ACATCTGTGT 
GGAATGGGAA AGAAAGACGA TCCAAGCTTA ATGCATCAGT GGTTCAAACT GGTGCAGGAG 
AAGAATGCTC TCGTGCGTTA TGAATCTGAG CTCATGATAT TCGCTCGTGA ACTGGAGCTG 
GAGGACAGAC AGAGTCGCCT GCAGCAGGAG CTGAGAGAGC GCATGGCGGT GGACGATCAT 
CTAAAAGGAG AAGAGGAGCT GGCGGAGGAG AGGCGTATAT TGAGCGAGAT GCTGGATGTG 
GTGGAGCAGA GGGATGCACT GGTGGCCCTC CTGGAGGAGC AGAGAGTTCG AGAAAAAGAG 
GAGGACAGTG ACCTGGAGGC TGTGATGCTG TCTAAGGGCT TCAGTCTGCA TTGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298467.1
CDS	295..5394
Translation	MWDGQSEMCQAHVLFDSFVQAATCKETLRAFQDLCEELNLNPGGQPQFYHTLRSRLHYWKAKALWAKLDKRACQKEYMRGHACTSTTCLIIGAGPCGLRTAIELGFLGARVVLVEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRKVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTAIRRQNYIHMYTGGVSSLALQIANQIQSQQAQAPKLLHRRESGSQKDLPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSSESDLEEEELVWKKGEELHARTNGERKLDLEEELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDEGEYCPWERERLSGVWLEEEEAGFVKESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNGSKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEADFWEDSSTSVLLDVHLCGMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	XM_009300192.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300192.2

ORF Insert Sequence:

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGAGGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAG GGTGAGTACT GCCCTTGGGA GAGGGAACGA 
CTCTCAGGGG TGTGGCTTGA GGAAGAGGAG GCGGGGTTTG TTAAAGAATC TTATGAAGGA 
TGCAGCGACG ACCCTGATAT AGACGTCAGC TCACTCAGTG AGTCCAAGCT ATGTGACCAA 
CGGGAACAGG AGGAGTCTGT TCCTTTCCAC CAATCCCCTG CCTCTACAGA ATCTCCAGTC 
TCAATGAAAC CTTCAGAATC AGACCTCACA CCTGATCCGT CAACAACCCC CGAGAGCTCG 
CCTGCCAAGA GGTCAGAGGT GGTGGAGGAG TTCTGGATGA AGAGTGCAGA GATCCGAAAG 
AGTTTAGGCC TGACTCCGCT TTCTAGAGAA TTGCAGCCAA AACATATAGC AGCTTCAACC 
CAGACATCTA ATGTTAAAGA AAGCTTTTAC ACCTCAGTCA CATATAACAC CGCTGCTTTG 
GATTCTCCAA ACCAAAGTGC TAGAATCTGT GACTCCAGCA CACAAACTCA TTCTGTGACA 
GATCTTCAAG AAACCTCCCC TCTTGGACCT ACAGATGGCG ATGCTGGCGT CGATTTAGGA 
CGTTGTTCTG TTGTTCACAG ACTCAGCATC ACTGTAGAAG GTTGTGTGAT GGCAGATAAC 
CAGGGTTTGG ATTCTACCTC GTTTGCTACT GAAAGTGTTT TAAACCCAGA CGCAGGTCTT 
CCCACCCCTC CTTATAGTCC GTCTCACTCC CCTCTTGTTG GTAAACAGTG TCGTGCCCTT 
CACCATTCTG AGCCAATTCT AGACAGAGAG GTTATGGCTT TCTCATCTAC CTGCACTGCT 
TCTGTACCAC AGCGGAGCAG TTTTAAATCA GACCTCGAGC AAGCTCAAAG TCTTCCACCA 
GATGAAATCG AGATCCTGTG CGGAGACGAG GCGGAAAAAC TCCCGGAAAG ATCTTGCAGG 
ATGGAGAGCG GAGAGGTGGA TAACCGTAGA GCGGAGCACA GCAGGACGCT TCCTGACCGG 
GTGGTCGCAC CGCTGCTGGC AGGGGGACCA GAGGTCAGGC TTCGCAGGTC TGAGATGAAG 
TTGTGGGGTC CAGACGCAGA TGGTGATGTG AAGGAGAAAA AGCGCAGCTC GTTGTTTTCA 
CCCCGTAAAA GCAGGAAGAA TGGAAATGCA GCAGCTGAGA GCGGGCGAGA GACAGGGAAG 
CACAAGTCCC TCTGGAAGAC TGTTTTTTCA GTGTATAAGA AGGATAAAAA GAGGAAGGAG 
GTGGCAGTGG TGGCGGAAAC ACTTCCTGCT GCTAATAATG GGAGCAAACG GAAAGTGTCG 
GGAATTAACA GAACCACAGA CCTGTGTTTC CGGAAGAATC CAAGTTTCTC TGAAGATACG 
GATATGTCCT GTCATGCTCT TCTTGAAAGA TGTCCTCTCC GAGCTCAGAG AGCAGGAACA 
GAGGAGGAAC TCAATGCCAG GCTGACACGT CGAGTGCAGA GAGCAGCGCG CAGACAGGCC 
AAGCAGGAGG AGCTGCGGAG GCTGCATAGA GCTCAGATAA TCCAGCGTCA GTTGGAGCAG 
GTGGAAGTGA AGCAGAGGCA GCTGGAGGAG AAAGGTGTGG CTGTGGAGAA AGCACTCAGA 
GGAGAAGCAG ACTTCTGGGA AGACTCTAGT ACCTCTGTCC TCTTGGATGT ACATCTGTGT 
GGAATGGGAA AGAAAGACGA TCCAAGCTTA ATGCATCAGT GGTTCAAACT GGTGCAGGAG 
AAGAATGCTC TCGTGCGTTA TGAATCTGAG CTCATGATAT TCGCTCGTGA ACTGGAGCTG 
GAGGACAGAC AGAGTCGCCT GCAGCAGGAG CTGAGAGAGC GCATGGCGGT GGACGATCAT 
CTAAAAGGAG AAGAGGAGCT GGCGGAGGAG AGGCGTATAT TGAGCGAGAT GCTGGATGTG 
GTGGAGCAGA GGGATGCACT GGTGGCCCTC CTGGAGGAGC AGAGAGTTCG AGAAAAAGAG 
GAGGACAGTG ACCTGGAGGC TGTGATGCTG TCTAAGGGCT TCAGTCTGCA TTGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( XM_009300192.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298467.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa44975
Clone ID Related Accession (Same CDS sequence)	XM_009300192.2 , XM_009300192.3
Accession Version	XM_009300192.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5100bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300192.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGAGGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAG GGTGAGTACT GCCCTTGGGA GAGGGAACGA 
CTCTCAGGGG TGTGGCTTGA GGAAGAGGAG GCGGGGTTTG TTAAAGAATC TTATGAAGGA 
TGCAGCGACG ACCCTGATAT AGACGTCAGC TCACTCAGTG AGTCCAAGCT ATGTGACCAA 
CGGGAACAGG AGGAGTCTGT TCCTTTCCAC CAATCCCCTG CCTCTACAGA ATCTCCAGTC 
TCAATGAAAC CTTCAGAATC AGACCTCACA CCTGATCCGT CAACAACCCC CGAGAGCTCG 
CCTGCCAAGA GGTCAGAGGT GGTGGAGGAG TTCTGGATGA AGAGTGCAGA GATCCGAAAG 
AGTTTAGGCC TGACTCCGCT TTCTAGAGAA TTGCAGCCAA AACATATAGC AGCTTCAACC 
CAGACATCTA ATGTTAAAGA AAGCTTTTAC ACCTCAGTCA CATATAACAC CGCTGCTTTG 
GATTCTCCAA ACCAAAGTGC TAGAATCTGT GACTCCAGCA CACAAACTCA TTCTGTGACA 
GATCTTCAAG AAACCTCCCC TCTTGGACCT ACAGATGGCG ATGCTGGCGT CGATTTAGGA 
CGTTGTTCTG TTGTTCACAG ACTCAGCATC ACTGTAGAAG GTTGTGTGAT GGCAGATAAC 
CAGGGTTTGG ATTCTACCTC GTTTGCTACT GAAAGTGTTT TAAACCCAGA CGCAGGTCTT 
CCCACCCCTC CTTATAGTCC GTCTCACTCC CCTCTTGTTG GTAAACAGTG TCGTGCCCTT 
CACCATTCTG AGCCAATTCT AGACAGAGAG GTTATGGCTT TCTCATCTAC CTGCACTGCT 
TCTGTACCAC AGCGGAGCAG TTTTAAATCA GACCTCGAGC AAGCTCAAAG TCTTCCACCA 
GATGAAATCG AGATCCTGTG CGGAGACGAG GCGGAAAAAC TCCCGGAAAG ATCTTGCAGG 
ATGGAGAGCG GAGAGGTGGA TAACCGTAGA GCGGAGCACA GCAGGACGCT TCCTGACCGG 
GTGGTCGCAC CGCTGCTGGC AGGGGGACCA GAGGTCAGGC TTCGCAGGTC TGAGATGAAG 
TTGTGGGGTC CAGACGCAGA TGGTGATGTG AAGGAGAAAA AGCGCAGCTC GTTGTTTTCA 
CCCCGTAAAA GCAGGAAGAA TGGAAATGCA GCAGCTGAGA GCGGGCGAGA GACAGGGAAG 
CACAAGTCCC TCTGGAAGAC TGTTTTTTCA GTGTATAAGA AGGATAAAAA GAGGAAGGAG 
GTGGCAGTGG TGGCGGAAAC ACTTCCTGCT GCTAATAATG GGAGCAAACG GAAAGTGTCG 
GGAATTAACA GAACCACAGA CCTGTGTTTC CGGAAGAATC CAAGTTTCTC TGAAGATACG 
GATATGTCCT GTCATGCTCT TCTTGAAAGA TGTCCTCTCC GAGCTCAGAG AGCAGGAACA 
GAGGAGGAAC TCAATGCCAG GCTGACACGT CGAGTGCAGA GAGCAGCGCG CAGACAGGCC 
AAGCAGGAGG AGCTGCGGAG GCTGCATAGA GCTCAGATAA TCCAGCGTCA GTTGGAGCAG 
GTGGAAGTGA AGCAGAGGCA GCTGGAGGAG AAAGGTGTGG CTGTGGAGAA AGCACTCAGA 
GGAGAAGCAG ACTTCTGGGA AGACTCTAGT ACCTCTGTCC TCTTGGATGT ACATCTGTGT 
GGAATGGGAA AGAAAGACGA TCCAAGCTTA ATGCATCAGT GGTTCAAACT GGTGCAGGAG 
AAGAATGCTC TCGTGCGTTA TGAATCTGAG CTCATGATAT TCGCTCGTGA ACTGGAGCTG 
GAGGACAGAC AGAGTCGCCT GCAGCAGGAG CTGAGAGAGC GCATGGCGGT GGACGATCAT 
CTAAAAGGAG AAGAGGAGCT GGCGGAGGAG AGGCGTATAT TGAGCGAGAT GCTGGATGTG 
GTGGAGCAGA GGGATGCACT GGTGGCCCTC CTGGAGGAGC AGAGAGTTCG AGAAAAAGAG 
GAGGACAGTG ACCTGGAGGC TGTGATGCTG TCTAAGGGCT TCAGTCTGCA TTGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298467.1
CDS	227..5326
Translation	MWDGQSEMCQAHVLFDSFVQAATCKETLRAFQDLCEELNLNPGGQPQFYHTLRSRLHYWKAKALWAKLDKRACQKEYMRGHACTSTTCLIIGAGPCGLRTAIELGFLGARVVLVEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRKVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTAIRRQNYIHMYTGGVSSLALQIANQIQSQQAQAPKLLHRRESGSQKDLPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSSESDLEEEELVWKKGEELHARTNGERKLDLEEELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDEGEYCPWERERLSGVWLEEEEAGFVKESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNGSKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEADFWEDSSTSVLLDVHLCGMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	XM_009300192.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300192.3

ORF Insert Sequence:

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGAGGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAG GGTGAGTACT GCCCTTGGGA GAGGGAACGA 
CTCTCAGGGG TGTGGCTTGA GGAAGAGGAG GCGGGGTTTG TTAAAGAATC TTATGAAGGA 
TGCAGCGACG ACCCTGATAT AGACGTCAGC TCACTCAGTG AGTCCAAGCT ATGTGACCAA 
CGGGAACAGG AGGAGTCTGT TCCTTTCCAC CAATCCCCTG CCTCTACAGA ATCTCCAGTC 
TCAATGAAAC CTTCAGAATC AGACCTCACA CCTGATCCGT CAACAACCCC CGAGAGCTCG 
CCTGCCAAGA GGTCAGAGGT GGTGGAGGAG TTCTGGATGA AGAGTGCAGA GATCCGAAAG 
AGTTTAGGCC TGACTCCGCT TTCTAGAGAA TTGCAGCCAA AACATATAGC AGCTTCAACC 
CAGACATCTA ATGTTAAAGA AAGCTTTTAC ACCTCAGTCA CATATAACAC CGCTGCTTTG 
GATTCTCCAA ACCAAAGTGC TAGAATCTGT GACTCCAGCA CACAAACTCA TTCTGTGACA 
GATCTTCAAG AAACCTCCCC TCTTGGACCT ACAGATGGCG ATGCTGGCGT CGATTTAGGA 
CGTTGTTCTG TTGTTCACAG ACTCAGCATC ACTGTAGAAG GTTGTGTGAT GGCAGATAAC 
CAGGGTTTGG ATTCTACCTC GTTTGCTACT GAAAGTGTTT TAAACCCAGA CGCAGGTCTT 
CCCACCCCTC CTTATAGTCC GTCTCACTCC CCTCTTGTTG GTAAACAGTG TCGTGCCCTT 
CACCATTCTG AGCCAATTCT AGACAGAGAG GTTATGGCTT TCTCATCTAC CTGCACTGCT 
TCTGTACCAC AGCGGAGCAG TTTTAAATCA GACCTCGAGC AAGCTCAAAG TCTTCCACCA 
GATGAAATCG AGATCCTGTG CGGAGACGAG GCGGAAAAAC TCCCGGAAAG ATCTTGCAGG 
ATGGAGAGCG GAGAGGTGGA TAACCGTAGA GCGGAGCACA GCAGGACGCT TCCTGACCGG 
GTGGTCGCAC CGCTGCTGGC AGGGGGACCA GAGGTCAGGC TTCGCAGGTC TGAGATGAAG 
TTGTGGGGTC CAGACGCAGA TGGTGATGTG AAGGAGAAAA AGCGCAGCTC GTTGTTTTCA 
CCCCGTAAAA GCAGGAAGAA TGGAAATGCA GCAGCTGAGA GCGGGCGAGA GACAGGGAAG 
CACAAGTCCC TCTGGAAGAC TGTTTTTTCA GTGTATAAGA AGGATAAAAA GAGGAAGGAG 
GTGGCAGTGG TGGCGGAAAC ACTTCCTGCT GCTAATAATG GGAGCAAACG GAAAGTGTCG 
GGAATTAACA GAACCACAGA CCTGTGTTTC CGGAAGAATC CAAGTTTCTC TGAAGATACG 
GATATGTCCT GTCATGCTCT TCTTGAAAGA TGTCCTCTCC GAGCTCAGAG AGCAGGAACA 
GAGGAGGAAC TCAATGCCAG GCTGACACGT CGAGTGCAGA GAGCAGCGCG CAGACAGGCC 
AAGCAGGAGG AGCTGCGGAG GCTGCATAGA GCTCAGATAA TCCAGCGTCA GTTGGAGCAG 
GTGGAAGTGA AGCAGAGGCA GCTGGAGGAG AAAGGTGTGG CTGTGGAGAA AGCACTCAGA 
GGAGAAGCAG ACTTCTGGGA AGACTCTAGT ACCTCTGTCC TCTTGGATGT ACATCTGTGT 
GGAATGGGAA AGAAAGACGA TCCAAGCTTA ATGCATCAGT GGTTCAAACT GGTGCAGGAG 
AAGAATGCTC TCGTGCGTTA TGAATCTGAG CTCATGATAT TCGCTCGTGA ACTGGAGCTG 
GAGGACAGAC AGAGTCGCCT GCAGCAGGAG CTGAGAGAGC GCATGGCGGT GGACGATCAT 
CTAAAAGGAG AAGAGGAGCT GGCGGAGGAG AGGCGTATAT TGAGCGAGAT GCTGGATGTG 
GTGGAGCAGA GGGATGCACT GGTGGCCCTC CTGGAGGAGC AGAGAGTTCG AGAAAAAGAG 
GAGGACAGTG ACCTGGAGGC TGTGATGCTG TCTAAGGGCT TCAGTCTGCA TTGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( XM_009300195.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298470.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X9, mRNA.

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CloneID	ODa44977
Clone ID Related Accession (Same CDS sequence)	XM_009300195.2 , XM_009300195.3
Accession Version	XM_009300195.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300195.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGAGGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAG GGTGAGTACT GCCCTTGGGA GAGGGAACGA 
CTCTCAGGGG TGTGGCTTGA GGAAGAGGAG GCGGGGTTTG TTAAAGAATC TTATGAAGGA 
TGCAGCGACG ACCCTGATAT AGACGTCAGC TCACTCAGTG AGTCCAAGCT ATGTGACCAA 
CGGGAACAGG AGGAGTCTGT TCCTTTCCAC CAATCCCCTG CCTCTACAGA ATCTCCAGTC 
TCAATGAAAC CTTCAGAATC AGACCTCACA CCTGATCCGT CAACAACCCC CGAGAGCTCG 
CCTGCCAAGA GGTCAGAGGT GGTGGAGGAG TTCTGGATGA AGAGTGCAGA GATCCGAAAG 
AGTTTAGGCC TGACTCCGCT TTCTAGAGAA TTGCAGCCAA AACATATAGC AGCTTCAACC 
CAGACATCTA ATGTTAAAGA AAGCTTTTAC ACCTCAGTCA CATATAACAC CGCTGCTTTG 
GATTCTCCAA ACCAAAGTGC TAGAATCTGT GACTCCAGCA CACAAACTCA TTCTGTGACA 
GATCTTCAAG AAACCTCCCC TCTTGGACCT ACAGATGGCG ATGCTGGCGT CGATTTAGGA 
CGTTGTTCTG TTGTTCACAG ACTCAGCATC ACTGTAGAAG GTTGTGTGAT GGCAGATAAC 
CAGGGTTTGG ATTCTACCTC GTTTGCTACT GAAAGTGTTT TAAACCCAGA CGCAGGTCTT 
CCCACCCCTC CTTATAGTCC GTCTCACTCC CCTCTTGTTG GTAAACAGTG TCGTGCCCTT 
CACCATTCTG AGCCAATTCT AGACAGAGAG GTTATGGCTT TCTCATCTAC CTGCACTGCT 
TCTGTACCAC AGCGGAGCAG TTTTAAATCA GACCTCGAGC AAGCTCAAAG TCTTCCACCA 
GATGAAATCG AGATCCTGTG CGGAGACGAG GCGGAAAAAC TCCCGGAAAG ATCTTGCAGG 
ATGGAGAGCG GAGAGGTGGA TAACCGTAGA GCGGAGCACA GCAGGACGCT TCCTGACCGG 
GTGGTCGCAC CGCTGCTGGC AGGGGGACCA GAGGTCAGGC TTCGCAGGTC TGAGATGAAG 
TTGTGGGGTC CAGACGCAGA TGGTGATGTG AAGGAGAAAA AGCGCAGCTC GTTGTTTTCA 
CCCCGTAAAA GCAGGAAGAA TGGAAATGCA GCAGCTGAGA GCGGGCGAGA GACAGGGAAG 
CACAAGTCCC TCTGGAAGAC TGTTTTTTCA GTGTATAAGA AGGATAAAAA GAGGAAGGAG 
GTGGCAGTGG TGGCGGAAAC ACTTCCTGCT GCTAATAATG GGAGCAAACG GAAAGTGTCG 
GGAATTAACA GAACCACAGA CCTGTGTTTC CGGAAGAATC CAAGTTTCTC TGAAGATACG 
GATATGTCCT GTCATGCTCT TCTTGAAAGA TGTCCTCTCC GAGCTCAGAG AGCAGGAACA 
GAGGAGGAAC TCAATGCCAG GCTGACACGT CGAGTGCAGA GAGCAGCGCG CAGACAGGCC 
AAGCAGGAGG AGCTGCGGAG GCTGCATAGA GCTCAGATAA TCCAGCGTCA GTTGGAGCAG 
GTGGAAGTGA AGCAGAGGCA GCTGGAGGAG AAAGGTGTGG CTGTGGAGAA AGCACTCAGA 
GGAGAAGCAG GAATGGGAAA GAAAGACGAT CCAAGCTTAA TGCATCAGTG GTTCAAACTG 
GTGCAGGAGA AGAATGCTCT CGTGCGTTAT GAATCTGAGC TCATGATATT CGCTCGTGAA 
CTGGAGCTGG AGGACAGACA GAGTCGCCTG CAGCAGGAGC TGAGAGAGCG CATGGCGGTG 
GACGATCATC TAAAAGGAGA AGAGGAGCTG GCGGAGGAGA GGCGTATATT GAGCGAGATG 
CTGGATGTGG TGGAGCAGAG GGATGCACTG GTGGCCCTCC TGGAGGAGCA GAGAGTTCGA 
GAAAAAGAGG AGGACAGTGA CCTGGAGGCT GTGATGCTGT CTAAGGGCTT CAGTCTGCAT 
TGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298470.1
CDS	295..5343
Translation	MWDGQSEMCQAHVLFDSFVQAATCKETLRAFQDLCEELNLNPGGQPQFYHTLRSRLHYWKAKALWAKLDKRACQKEYMRGHACTSTTCLIIGAGPCGLRTAIELGFLGARVVLVEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRKVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTAIRRQNYIHMYTGGVSSLALQIANQIQSQQAQAPKLLHRRESGSQKDLPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSSESDLEEEELVWKKGEELHARTNGERKLDLEEELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDEGEYCPWERERLSGVWLEEEEAGFVKESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNGSKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEAGMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	XM_009300195.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300195.2

ORF Insert Sequence:

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGAGGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAG GGTGAGTACT GCCCTTGGGA GAGGGAACGA 
CTCTCAGGGG TGTGGCTTGA GGAAGAGGAG GCGGGGTTTG TTAAAGAATC TTATGAAGGA 
TGCAGCGACG ACCCTGATAT AGACGTCAGC TCACTCAGTG AGTCCAAGCT ATGTGACCAA 
CGGGAACAGG AGGAGTCTGT TCCTTTCCAC CAATCCCCTG CCTCTACAGA ATCTCCAGTC 
TCAATGAAAC CTTCAGAATC AGACCTCACA CCTGATCCGT CAACAACCCC CGAGAGCTCG 
CCTGCCAAGA GGTCAGAGGT GGTGGAGGAG TTCTGGATGA AGAGTGCAGA GATCCGAAAG 
AGTTTAGGCC TGACTCCGCT TTCTAGAGAA TTGCAGCCAA AACATATAGC AGCTTCAACC 
CAGACATCTA ATGTTAAAGA AAGCTTTTAC ACCTCAGTCA CATATAACAC CGCTGCTTTG 
GATTCTCCAA ACCAAAGTGC TAGAATCTGT GACTCCAGCA CACAAACTCA TTCTGTGACA 
GATCTTCAAG AAACCTCCCC TCTTGGACCT ACAGATGGCG ATGCTGGCGT CGATTTAGGA 
CGTTGTTCTG TTGTTCACAG ACTCAGCATC ACTGTAGAAG GTTGTGTGAT GGCAGATAAC 
CAGGGTTTGG ATTCTACCTC GTTTGCTACT GAAAGTGTTT TAAACCCAGA CGCAGGTCTT 
CCCACCCCTC CTTATAGTCC GTCTCACTCC CCTCTTGTTG GTAAACAGTG TCGTGCCCTT 
CACCATTCTG AGCCAATTCT AGACAGAGAG GTTATGGCTT TCTCATCTAC CTGCACTGCT 
TCTGTACCAC AGCGGAGCAG TTTTAAATCA GACCTCGAGC AAGCTCAAAG TCTTCCACCA 
GATGAAATCG AGATCCTGTG CGGAGACGAG GCGGAAAAAC TCCCGGAAAG ATCTTGCAGG 
ATGGAGAGCG GAGAGGTGGA TAACCGTAGA GCGGAGCACA GCAGGACGCT TCCTGACCGG 
GTGGTCGCAC CGCTGCTGGC AGGGGGACCA GAGGTCAGGC TTCGCAGGTC TGAGATGAAG 
TTGTGGGGTC CAGACGCAGA TGGTGATGTG AAGGAGAAAA AGCGCAGCTC GTTGTTTTCA 
CCCCGTAAAA GCAGGAAGAA TGGAAATGCA GCAGCTGAGA GCGGGCGAGA GACAGGGAAG 
CACAAGTCCC TCTGGAAGAC TGTTTTTTCA GTGTATAAGA AGGATAAAAA GAGGAAGGAG 
GTGGCAGTGG TGGCGGAAAC ACTTCCTGCT GCTAATAATG GGAGCAAACG GAAAGTGTCG 
GGAATTAACA GAACCACAGA CCTGTGTTTC CGGAAGAATC CAAGTTTCTC TGAAGATACG 
GATATGTCCT GTCATGCTCT TCTTGAAAGA TGTCCTCTCC GAGCTCAGAG AGCAGGAACA 
GAGGAGGAAC TCAATGCCAG GCTGACACGT CGAGTGCAGA GAGCAGCGCG CAGACAGGCC 
AAGCAGGAGG AGCTGCGGAG GCTGCATAGA GCTCAGATAA TCCAGCGTCA GTTGGAGCAG 
GTGGAAGTGA AGCAGAGGCA GCTGGAGGAG AAAGGTGTGG CTGTGGAGAA AGCACTCAGA 
GGAGAAGCAG GAATGGGAAA GAAAGACGAT CCAAGCTTAA TGCATCAGTG GTTCAAACTG 
GTGCAGGAGA AGAATGCTCT CGTGCGTTAT GAATCTGAGC TCATGATATT CGCTCGTGAA 
CTGGAGCTGG AGGACAGACA GAGTCGCCTG CAGCAGGAGC TGAGAGAGCG CATGGCGGTG 
GACGATCATC TAAAAGGAGA AGAGGAGCTG GCGGAGGAGA GGCGTATATT GAGCGAGATG 
CTGGATGTGG TGGAGCAGAG GGATGCACTG GTGGCCCTCC TGGAGGAGCA GAGAGTTCGA 
GAAAAAGAGG AGGACAGTGA CCTGGAGGCT GTGATGCTGT CTAAGGGCTT CAGTCTGCAT 
TGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( XM_009300195.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298470.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X6, mRNA.

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CloneID	ODa44977
Clone ID Related Accession (Same CDS sequence)	XM_009300195.2 , XM_009300195.3
Accession Version	XM_009300195.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5049bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300195.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGAGGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAG GGTGAGTACT GCCCTTGGGA GAGGGAACGA 
CTCTCAGGGG TGTGGCTTGA GGAAGAGGAG GCGGGGTTTG TTAAAGAATC TTATGAAGGA 
TGCAGCGACG ACCCTGATAT AGACGTCAGC TCACTCAGTG AGTCCAAGCT ATGTGACCAA 
CGGGAACAGG AGGAGTCTGT TCCTTTCCAC CAATCCCCTG CCTCTACAGA ATCTCCAGTC 
TCAATGAAAC CTTCAGAATC AGACCTCACA CCTGATCCGT CAACAACCCC CGAGAGCTCG 
CCTGCCAAGA GGTCAGAGGT GGTGGAGGAG TTCTGGATGA AGAGTGCAGA GATCCGAAAG 
AGTTTAGGCC TGACTCCGCT TTCTAGAGAA TTGCAGCCAA AACATATAGC AGCTTCAACC 
CAGACATCTA ATGTTAAAGA AAGCTTTTAC ACCTCAGTCA CATATAACAC CGCTGCTTTG 
GATTCTCCAA ACCAAAGTGC TAGAATCTGT GACTCCAGCA CACAAACTCA TTCTGTGACA 
GATCTTCAAG AAACCTCCCC TCTTGGACCT ACAGATGGCG ATGCTGGCGT CGATTTAGGA 
CGTTGTTCTG TTGTTCACAG ACTCAGCATC ACTGTAGAAG GTTGTGTGAT GGCAGATAAC 
CAGGGTTTGG ATTCTACCTC GTTTGCTACT GAAAGTGTTT TAAACCCAGA CGCAGGTCTT 
CCCACCCCTC CTTATAGTCC GTCTCACTCC CCTCTTGTTG GTAAACAGTG TCGTGCCCTT 
CACCATTCTG AGCCAATTCT AGACAGAGAG GTTATGGCTT TCTCATCTAC CTGCACTGCT 
TCTGTACCAC AGCGGAGCAG TTTTAAATCA GACCTCGAGC AAGCTCAAAG TCTTCCACCA 
GATGAAATCG AGATCCTGTG CGGAGACGAG GCGGAAAAAC TCCCGGAAAG ATCTTGCAGG 
ATGGAGAGCG GAGAGGTGGA TAACCGTAGA GCGGAGCACA GCAGGACGCT TCCTGACCGG 
GTGGTCGCAC CGCTGCTGGC AGGGGGACCA GAGGTCAGGC TTCGCAGGTC TGAGATGAAG 
TTGTGGGGTC CAGACGCAGA TGGTGATGTG AAGGAGAAAA AGCGCAGCTC GTTGTTTTCA 
CCCCGTAAAA GCAGGAAGAA TGGAAATGCA GCAGCTGAGA GCGGGCGAGA GACAGGGAAG 
CACAAGTCCC TCTGGAAGAC TGTTTTTTCA GTGTATAAGA AGGATAAAAA GAGGAAGGAG 
GTGGCAGTGG TGGCGGAAAC ACTTCCTGCT GCTAATAATG GGAGCAAACG GAAAGTGTCG 
GGAATTAACA GAACCACAGA CCTGTGTTTC CGGAAGAATC CAAGTTTCTC TGAAGATACG 
GATATGTCCT GTCATGCTCT TCTTGAAAGA TGTCCTCTCC GAGCTCAGAG AGCAGGAACA 
GAGGAGGAAC TCAATGCCAG GCTGACACGT CGAGTGCAGA GAGCAGCGCG CAGACAGGCC 
AAGCAGGAGG AGCTGCGGAG GCTGCATAGA GCTCAGATAA TCCAGCGTCA GTTGGAGCAG 
GTGGAAGTGA AGCAGAGGCA GCTGGAGGAG AAAGGTGTGG CTGTGGAGAA AGCACTCAGA 
GGAGAAGCAG GAATGGGAAA GAAAGACGAT CCAAGCTTAA TGCATCAGTG GTTCAAACTG 
GTGCAGGAGA AGAATGCTCT CGTGCGTTAT GAATCTGAGC TCATGATATT CGCTCGTGAA 
CTGGAGCTGG AGGACAGACA GAGTCGCCTG CAGCAGGAGC TGAGAGAGCG CATGGCGGTG 
GACGATCATC TAAAAGGAGA AGAGGAGCTG GCGGAGGAGA GGCGTATATT GAGCGAGATG 
CTGGATGTGG TGGAGCAGAG GGATGCACTG GTGGCCCTCC TGGAGGAGCA GAGAGTTCGA 
GAAAAAGAGG AGGACAGTGA CCTGGAGGCT GTGATGCTGT CTAAGGGCTT CAGTCTGCAT 
TGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298470.1
CDS	227..5275
Translation	MWDGQSEMCQAHVLFDSFVQAATCKETLRAFQDLCEELNLNPGGQPQFYHTLRSRLHYWKAKALWAKLDKRACQKEYMRGHACTSTTCLIIGAGPCGLRTAIELGFLGARVVLVEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRKVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTAIRRQNYIHMYTGGVSSLALQIANQIQSQQAQAPKLLHRRESGSQKDLPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSSESDLEEEELVWKKGEELHARTNGERKLDLEEELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDEGEYCPWERERLSGVWLEEEEAGFVKESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNGSKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEAGMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	XM_009300195.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300195.3

ORF Insert Sequence:

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGAGGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAG GGTGAGTACT GCCCTTGGGA GAGGGAACGA 
CTCTCAGGGG TGTGGCTTGA GGAAGAGGAG GCGGGGTTTG TTAAAGAATC TTATGAAGGA 
TGCAGCGACG ACCCTGATAT AGACGTCAGC TCACTCAGTG AGTCCAAGCT ATGTGACCAA 
CGGGAACAGG AGGAGTCTGT TCCTTTCCAC CAATCCCCTG CCTCTACAGA ATCTCCAGTC 
TCAATGAAAC CTTCAGAATC AGACCTCACA CCTGATCCGT CAACAACCCC CGAGAGCTCG 
CCTGCCAAGA GGTCAGAGGT GGTGGAGGAG TTCTGGATGA AGAGTGCAGA GATCCGAAAG 
AGTTTAGGCC TGACTCCGCT TTCTAGAGAA TTGCAGCCAA AACATATAGC AGCTTCAACC 
CAGACATCTA ATGTTAAAGA AAGCTTTTAC ACCTCAGTCA CATATAACAC CGCTGCTTTG 
GATTCTCCAA ACCAAAGTGC TAGAATCTGT GACTCCAGCA CACAAACTCA TTCTGTGACA 
GATCTTCAAG AAACCTCCCC TCTTGGACCT ACAGATGGCG ATGCTGGCGT CGATTTAGGA 
CGTTGTTCTG TTGTTCACAG ACTCAGCATC ACTGTAGAAG GTTGTGTGAT GGCAGATAAC 
CAGGGTTTGG ATTCTACCTC GTTTGCTACT GAAAGTGTTT TAAACCCAGA CGCAGGTCTT 
CCCACCCCTC CTTATAGTCC GTCTCACTCC CCTCTTGTTG GTAAACAGTG TCGTGCCCTT 
CACCATTCTG AGCCAATTCT AGACAGAGAG GTTATGGCTT TCTCATCTAC CTGCACTGCT 
TCTGTACCAC AGCGGAGCAG TTTTAAATCA GACCTCGAGC AAGCTCAAAG TCTTCCACCA 
GATGAAATCG AGATCCTGTG CGGAGACGAG GCGGAAAAAC TCCCGGAAAG ATCTTGCAGG 
ATGGAGAGCG GAGAGGTGGA TAACCGTAGA GCGGAGCACA GCAGGACGCT TCCTGACCGG 
GTGGTCGCAC CGCTGCTGGC AGGGGGACCA GAGGTCAGGC TTCGCAGGTC TGAGATGAAG 
TTGTGGGGTC CAGACGCAGA TGGTGATGTG AAGGAGAAAA AGCGCAGCTC GTTGTTTTCA 
CCCCGTAAAA GCAGGAAGAA TGGAAATGCA GCAGCTGAGA GCGGGCGAGA GACAGGGAAG 
CACAAGTCCC TCTGGAAGAC TGTTTTTTCA GTGTATAAGA AGGATAAAAA GAGGAAGGAG 
GTGGCAGTGG TGGCGGAAAC ACTTCCTGCT GCTAATAATG GGAGCAAACG GAAAGTGTCG 
GGAATTAACA GAACCACAGA CCTGTGTTTC CGGAAGAATC CAAGTTTCTC TGAAGATACG 
GATATGTCCT GTCATGCTCT TCTTGAAAGA TGTCCTCTCC GAGCTCAGAG AGCAGGAACA 
GAGGAGGAAC TCAATGCCAG GCTGACACGT CGAGTGCAGA GAGCAGCGCG CAGACAGGCC 
AAGCAGGAGG AGCTGCGGAG GCTGCATAGA GCTCAGATAA TCCAGCGTCA GTTGGAGCAG 
GTGGAAGTGA AGCAGAGGCA GCTGGAGGAG AAAGGTGTGG CTGTGGAGAA AGCACTCAGA 
GGAGAAGCAG GAATGGGAAA GAAAGACGAT CCAAGCTTAA TGCATCAGTG GTTCAAACTG 
GTGCAGGAGA AGAATGCTCT CGTGCGTTAT GAATCTGAGC TCATGATATT CGCTCGTGAA 
CTGGAGCTGG AGGACAGACA GAGTCGCCTG CAGCAGGAGC TGAGAGAGCG CATGGCGGTG 
GACGATCATC TAAAAGGAGA AGAGGAGCTG GCGGAGGAGA GGCGTATATT GAGCGAGATG 
CTGGATGTGG TGGAGCAGAG GGATGCACTG GTGGCCCTCC TGGAGGAGCA GAGAGTTCGA 
GAAAAAGAGG AGGACAGTGA CCTGGAGGCT GTGATGCTGT CTAAGGGCTT CAGTCTGCAT 
TGGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( XM_017355273.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210762.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X1, mRNA.

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CloneID	ODa58545
Clone ID Related Accession (Same CDS sequence)	XM_017355273.1 , XM_017355274.1
Accession Version	XM_017355273.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGAGGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAG GGTGAGTACT GCCCTTGGGA GAGGGAACGA 
CTCTCAGGGG TGTGGCTTGA GGAAGAGGAG GCGGGGTTTG TTAAAGAATC TTATGAAGGA 
TGCAGCGACG ACCCTGATAT AGACGTCAGC TCACTCAGTG AGTCCAAGCT ATGTGACCAA 
CGGGAACAGG AGGAGTCTGT TCCTTTCCAC CAATCCCCTG CCTCTACAGA ATCTCCAGTC 
TCAATGAAAC CTTCAGAATC AGACCTCACA CCTGATCCGT CAACAACCCC CGAGAGCTCG 
CCTGCCAAGA GGTCAGAGGT GGTGGAGGAG TTCTGGATGA AGAGTGCAGA GATCCGAAAG 
AGTTTAGGCC TGACTCCGCT TTCTAGAGAA TTGCAGCCAA AACATATAGC AGCTTCAACC 
CAGACATCTA ATGTTAAAGA AAGCTTTTAC ACCTCAGTCA CATATAACAC CGCTGCTTTG 
GATTCTCCAA ACCAAAGTGC TAGAATCTGT GACTCCAGCA CACAAACTCA TTCTGTGACA 
GATCTTCAAG AAACCTCCCC TCTTGGACCT ACAGATGGCG ATGCTGGCGT CGATTTAGGA 
CGTTGTTCTG TTGTTCACAG ACTCAGCATC ACTGTAGAAG GTTGTGTGAT GGCAGATAAC 
CAGGGTTTGG ATTCTACCTC GTTTGCTACT GAAAGTGTTT TAAACCCAGA CGCAGGTCTT 
CCCACCCCTC CTTATAGTCC GTCTCACTCC CCTCTTGTTG GTAAACAGTG TCGTGCCCTT 
CACCATTCTG AGCCAATTCT AGACAGAGAG GTTATGGCTT TCTCATCTAC CTGCACTGCT 
TCTGTACCAC AGCGGAGCAG TTTTAAATCA GACCTCGAGC AAGCTCAAAG TCTTCCACCA 
GATGAAATCG AGATCCTGTG CGGAGACGAG GCGGAAAAAC TCCCGGAAAG ATCTTGCAGG 
ATGGAGAGCG GAGAGGTGGA TAACCGTAGA GCGGAGCACA GCAGGACGCT TCCTGACCGG 
GTGGTCGCAC CGCTGCTGGC AGGGGGACCA GAGGTCAGGC TTCGCAGGTC TGAGATGAAG 
TTGTGGGGTC CAGACGCAGA TGGTGATGTG AAGGAGAAAA AGCGCAGCTC GTTGTTTTCA 
CCCCGTAAAA GCAGGAAGAA TGGAAATGCA GCAGCTGAGA GCGGGCGAGA GACAGGGAAG 
CACAAGTCCC TCTGGAAGAC TGTTTTTTCA GTGTATAAGA AGGATAAAAA GAGGAAGGAG 
GTGGCAGTGG TGGCGGAAAC ACTTCCTGCT GCTAATAATG GGAGCAAACG GAAAGTGTCG 
GGAATTAACA GAACCACAGA CCTGTGTTTC CGGAAGAATC CAAGTTTCTC TGAAGATACG 
GATATGTCCT GTCATGCTCT TCTTGAAAGA TGTCCTCTCC GAGCTCAGAG AGCAGGAACA 
GAGGAGGAAC TCAATGCCAG GCTGACACGT CGAGTGCAGA GAGCAGCGCG CAGACAGGCC 
AAGCAGGAGG AGCTGCGGAG GCTGCATAGA GCTCAGATAA TCCAGCGTCA GTTGGAGCAG 
GTGGAAGTGA AGCAGAGGCA GCTGGAGGAG AAAGGTGTGG CTGTGGAGAA AGCACTCAGA 
GGAGAAGCAG TTGAGCCTTT TGACGGTTCT CCCCGTAGAA GGCCTGTATA TCTCTGTCCT 
TGCTGTTCCT CTGAAGGTAA AACCGGCCCT GCTTCCTTTC TCACTCCTCT AGTGCAGATT 
TTAGCAATAC TTTACAATCC TTTTTACCTT TTCTTCCTCT CTGCTCATTC GTCCTGCCTC 
CTGATGTGTG CAGCCAAAGC TAAAGGAACC GAACATGATC ATTCGGTTTA TGTCTGTACA 
GTCTTTTTCC TGTACGTAGT GCAGGAGCCT CCACTTCACC AACTGAGAAT GGGAAAGAAA 
GACGATCCAA GCTTAATGCA TCAGTGGTTC AAACTGGTGC AGGAGAAGAA TGCTCTCGTG 
CGTTATGAAT CTGAGCTCAT GATATTCGCT CGTGAACTGG AGCTGGAGGA CAGACAGAGT 
CGCCTGCAGC AGGAGCTGAG AGAGCGCATG GCGGTGGACG ATCATCTAAA AGGAGAAGAG 
GAGCTGGCGG AGGAGAGGCG TATATTGAGC GAGATGCTGG ATGTGGTGGA GCAGAGGGAT 
GCACTGGTGG CCCTCCTGGA GGAGCAGAGA GTTCGAGAAA AAGAGGAGGA CAGTGACCTG 
GAGGCTGTGA TGCTGTCTAA GGGCTTCAGT CTGCATTGGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210762.1
CDS	296..5620
Translation	MWDGQSEMCQAHVLFDSFVQAATCKETLRAFQDLCEELNLNPGGQPQFYHTLRSRLHYWKAKALWAKLDKRACQKEYMRGHACTSTTCLIIGAGPCGLRTAIELGFLGARVVLVEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRKVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTAIRRQNYIHMYTGGVSSLALQIANQIQSQQAQAPKLLHRRESGSQKDLPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSSESDLEEEELVWKKGEELHARTNGERKLDLEEELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDEGEYCPWERERLSGVWLEEEEAGFVKESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNGSKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEAVEPFDGSPRRRPVYLCPCCSSEGKTGPASFLTPLVQILAILYNPFYLFFLSAHSSCLLMCAAKAKGTEHDHSVYVCTVFFLYVVQEPPLHQLRMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	XM_017355273.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355273.1

ORF Insert Sequence:

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGAGGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAG GGTGAGTACT GCCCTTGGGA GAGGGAACGA 
CTCTCAGGGG TGTGGCTTGA GGAAGAGGAG GCGGGGTTTG TTAAAGAATC TTATGAAGGA 
TGCAGCGACG ACCCTGATAT AGACGTCAGC TCACTCAGTG AGTCCAAGCT ATGTGACCAA 
CGGGAACAGG AGGAGTCTGT TCCTTTCCAC CAATCCCCTG CCTCTACAGA ATCTCCAGTC 
TCAATGAAAC CTTCAGAATC AGACCTCACA CCTGATCCGT CAACAACCCC CGAGAGCTCG 
CCTGCCAAGA GGTCAGAGGT GGTGGAGGAG TTCTGGATGA AGAGTGCAGA GATCCGAAAG 
AGTTTAGGCC TGACTCCGCT TTCTAGAGAA TTGCAGCCAA AACATATAGC AGCTTCAACC 
CAGACATCTA ATGTTAAAGA AAGCTTTTAC ACCTCAGTCA CATATAACAC CGCTGCTTTG 
GATTCTCCAA ACCAAAGTGC TAGAATCTGT GACTCCAGCA CACAAACTCA TTCTGTGACA 
GATCTTCAAG AAACCTCCCC TCTTGGACCT ACAGATGGCG ATGCTGGCGT CGATTTAGGA 
CGTTGTTCTG TTGTTCACAG ACTCAGCATC ACTGTAGAAG GTTGTGTGAT GGCAGATAAC 
CAGGGTTTGG ATTCTACCTC GTTTGCTACT GAAAGTGTTT TAAACCCAGA CGCAGGTCTT 
CCCACCCCTC CTTATAGTCC GTCTCACTCC CCTCTTGTTG GTAAACAGTG TCGTGCCCTT 
CACCATTCTG AGCCAATTCT AGACAGAGAG GTTATGGCTT TCTCATCTAC CTGCACTGCT 
TCTGTACCAC AGCGGAGCAG TTTTAAATCA GACCTCGAGC AAGCTCAAAG TCTTCCACCA 
GATGAAATCG AGATCCTGTG CGGAGACGAG GCGGAAAAAC TCCCGGAAAG ATCTTGCAGG 
ATGGAGAGCG GAGAGGTGGA TAACCGTAGA GCGGAGCACA GCAGGACGCT TCCTGACCGG 
GTGGTCGCAC CGCTGCTGGC AGGGGGACCA GAGGTCAGGC TTCGCAGGTC TGAGATGAAG 
TTGTGGGGTC CAGACGCAGA TGGTGATGTG AAGGAGAAAA AGCGCAGCTC GTTGTTTTCA 
CCCCGTAAAA GCAGGAAGAA TGGAAATGCA GCAGCTGAGA GCGGGCGAGA GACAGGGAAG 
CACAAGTCCC TCTGGAAGAC TGTTTTTTCA GTGTATAAGA AGGATAAAAA GAGGAAGGAG 
GTGGCAGTGG TGGCGGAAAC ACTTCCTGCT GCTAATAATG GGAGCAAACG GAAAGTGTCG 
GGAATTAACA GAACCACAGA CCTGTGTTTC CGGAAGAATC CAAGTTTCTC TGAAGATACG 
GATATGTCCT GTCATGCTCT TCTTGAAAGA TGTCCTCTCC GAGCTCAGAG AGCAGGAACA 
GAGGAGGAAC TCAATGCCAG GCTGACACGT CGAGTGCAGA GAGCAGCGCG CAGACAGGCC 
AAGCAGGAGG AGCTGCGGAG GCTGCATAGA GCTCAGATAA TCCAGCGTCA GTTGGAGCAG 
GTGGAAGTGA AGCAGAGGCA GCTGGAGGAG AAAGGTGTGG CTGTGGAGAA AGCACTCAGA 
GGAGAAGCAG TTGAGCCTTT TGACGGTTCT CCCCGTAGAA GGCCTGTATA TCTCTGTCCT 
TGCTGTTCCT CTGAAGGTAA AACCGGCCCT GCTTCCTTTC TCACTCCTCT AGTGCAGATT 
TTAGCAATAC TTTACAATCC TTTTTACCTT TTCTTCCTCT CTGCTCATTC GTCCTGCCTC 
CTGATGTGTG CAGCCAAAGC TAAAGGAACC GAACATGATC ATTCGGTTTA TGTCTGTACA 
GTCTTTTTCC TGTACGTAGT GCAGGAGCCT CCACTTCACC AACTGAGAAT GGGAAAGAAA 
GACGATCCAA GCTTAATGCA TCAGTGGTTC AAACTGGTGC AGGAGAAGAA TGCTCTCGTG 
CGTTATGAAT CTGAGCTCAT GATATTCGCT CGTGAACTGG AGCTGGAGGA CAGACAGAGT 
CGCCTGCAGC AGGAGCTGAG AGAGCGCATG GCGGTGGACG ATCATCTAAA AGGAGAAGAG 
GAGCTGGCGG AGGAGAGGCG TATATTGAGC GAGATGCTGG ATGTGGTGGA GCAGAGGGAT 
GCACTGGTGG CCCTCCTGGA GGAGCAGAGA GTTCGAGAAA AAGAGGAGGA CAGTGACCTG 
GAGGCTGTGA TGCTGTCTAA GGGCTTCAGT CTGCATTGGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( XM_017355274.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210763.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X2, mRNA.

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CloneID	ODa58545
Clone ID Related Accession (Same CDS sequence)	XM_017355273.1 , XM_017355274.1
Accession Version	XM_017355274.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5325bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGAGGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAG GGTGAGTACT GCCCTTGGGA GAGGGAACGA 
CTCTCAGGGG TGTGGCTTGA GGAAGAGGAG GCGGGGTTTG TTAAAGAATC TTATGAAGGA 
TGCAGCGACG ACCCTGATAT AGACGTCAGC TCACTCAGTG AGTCCAAGCT ATGTGACCAA 
CGGGAACAGG AGGAGTCTGT TCCTTTCCAC CAATCCCCTG CCTCTACAGA ATCTCCAGTC 
TCAATGAAAC CTTCAGAATC AGACCTCACA CCTGATCCGT CAACAACCCC CGAGAGCTCG 
CCTGCCAAGA GGTCAGAGGT GGTGGAGGAG TTCTGGATGA AGAGTGCAGA GATCCGAAAG 
AGTTTAGGCC TGACTCCGCT TTCTAGAGAA TTGCAGCCAA AACATATAGC AGCTTCAACC 
CAGACATCTA ATGTTAAAGA AAGCTTTTAC ACCTCAGTCA CATATAACAC CGCTGCTTTG 
GATTCTCCAA ACCAAAGTGC TAGAATCTGT GACTCCAGCA CACAAACTCA TTCTGTGACA 
GATCTTCAAG AAACCTCCCC TCTTGGACCT ACAGATGGCG ATGCTGGCGT CGATTTAGGA 
CGTTGTTCTG TTGTTCACAG ACTCAGCATC ACTGTAGAAG GTTGTGTGAT GGCAGATAAC 
CAGGGTTTGG ATTCTACCTC GTTTGCTACT GAAAGTGTTT TAAACCCAGA CGCAGGTCTT 
CCCACCCCTC CTTATAGTCC GTCTCACTCC CCTCTTGTTG GTAAACAGTG TCGTGCCCTT 
CACCATTCTG AGCCAATTCT AGACAGAGAG GTTATGGCTT TCTCATCTAC CTGCACTGCT 
TCTGTACCAC AGCGGAGCAG TTTTAAATCA GACCTCGAGC AAGCTCAAAG TCTTCCACCA 
GATGAAATCG AGATCCTGTG CGGAGACGAG GCGGAAAAAC TCCCGGAAAG ATCTTGCAGG 
ATGGAGAGCG GAGAGGTGGA TAACCGTAGA GCGGAGCACA GCAGGACGCT TCCTGACCGG 
GTGGTCGCAC CGCTGCTGGC AGGGGGACCA GAGGTCAGGC TTCGCAGGTC TGAGATGAAG 
TTGTGGGGTC CAGACGCAGA TGGTGATGTG AAGGAGAAAA AGCGCAGCTC GTTGTTTTCA 
CCCCGTAAAA GCAGGAAGAA TGGAAATGCA GCAGCTGAGA GCGGGCGAGA GACAGGGAAG 
CACAAGTCCC TCTGGAAGAC TGTTTTTTCA GTGTATAAGA AGGATAAAAA GAGGAAGGAG 
GTGGCAGTGG TGGCGGAAAC ACTTCCTGCT GCTAATAATG GGAGCAAACG GAAAGTGTCG 
GGAATTAACA GAACCACAGA CCTGTGTTTC CGGAAGAATC CAAGTTTCTC TGAAGATACG 
GATATGTCCT GTCATGCTCT TCTTGAAAGA TGTCCTCTCC GAGCTCAGAG AGCAGGAACA 
GAGGAGGAAC TCAATGCCAG GCTGACACGT CGAGTGCAGA GAGCAGCGCG CAGACAGGCC 
AAGCAGGAGG AGCTGCGGAG GCTGCATAGA GCTCAGATAA TCCAGCGTCA GTTGGAGCAG 
GTGGAAGTGA AGCAGAGGCA GCTGGAGGAG AAAGGTGTGG CTGTGGAGAA AGCACTCAGA 
GGAGAAGCAG TTGAGCCTTT TGACGGTTCT CCCCGTAGAA GGCCTGTATA TCTCTGTCCT 
TGCTGTTCCT CTGAAGGTAA AACCGGCCCT GCTTCCTTTC TCACTCCTCT AGTGCAGATT 
TTAGCAATAC TTTACAATCC TTTTTACCTT TTCTTCCTCT CTGCTCATTC GTCCTGCCTC 
CTGATGTGTG CAGCCAAAGC TAAAGGAACC GAACATGATC ATTCGGTTTA TGTCTGTACA 
GTCTTTTTCC TGTACGTAGT GCAGGAGCCT CCACTTCACC AACTGAGAAT GGGAAAGAAA 
GACGATCCAA GCTTAATGCA TCAGTGGTTC AAACTGGTGC AGGAGAAGAA TGCTCTCGTG 
CGTTATGAAT CTGAGCTCAT GATATTCGCT CGTGAACTGG AGCTGGAGGA CAGACAGAGT 
CGCCTGCAGC AGGAGCTGAG AGAGCGCATG GCGGTGGACG ATCATCTAAA AGGAGAAGAG 
GAGCTGGCGG AGGAGAGGCG TATATTGAGC GAGATGCTGG ATGTGGTGGA GCAGAGGGAT 
GCACTGGTGG CCCTCCTGGA GGAGCAGAGA GTTCGAGAAA AAGAGGAGGA CAGTGACCTG 
GAGGCTGTGA TGCTGTCTAA GGGCTTCAGT CTGCATTGGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210763.1
CDS	299..5623
Translation	MWDGQSEMCQAHVLFDSFVQAATCKETLRAFQDLCEELNLNPGGQPQFYHTLRSRLHYWKAKALWAKLDKRACQKEYMRGHACTSTTCLIIGAGPCGLRTAIELGFLGARVVLVEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRKVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTAIRRQNYIHMYTGGVSSLALQIANQIQSQQAQAPKLLHRRESGSQKDLPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSSESDLEEEELVWKKGEELHARTNGERKLDLEEELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDEGEYCPWERERLSGVWLEEEEAGFVKESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNGSKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEAVEPFDGSPRRRPVYLCPCCSSEGKTGPASFLTPLVQILAILYNPFYLFFLSAHSSCLLMCAAKAKGTEHDHSVYVCTVFFLYVVQEPPLHQLRMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	XM_017355274.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355274.1

ORF Insert Sequence:

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGAGGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAG GGTGAGTACT GCCCTTGGGA GAGGGAACGA 
CTCTCAGGGG TGTGGCTTGA GGAAGAGGAG GCGGGGTTTG TTAAAGAATC TTATGAAGGA 
TGCAGCGACG ACCCTGATAT AGACGTCAGC TCACTCAGTG AGTCCAAGCT ATGTGACCAA 
CGGGAACAGG AGGAGTCTGT TCCTTTCCAC CAATCCCCTG CCTCTACAGA ATCTCCAGTC 
TCAATGAAAC CTTCAGAATC AGACCTCACA CCTGATCCGT CAACAACCCC CGAGAGCTCG 
CCTGCCAAGA GGTCAGAGGT GGTGGAGGAG TTCTGGATGA AGAGTGCAGA GATCCGAAAG 
AGTTTAGGCC TGACTCCGCT TTCTAGAGAA TTGCAGCCAA AACATATAGC AGCTTCAACC 
CAGACATCTA ATGTTAAAGA AAGCTTTTAC ACCTCAGTCA CATATAACAC CGCTGCTTTG 
GATTCTCCAA ACCAAAGTGC TAGAATCTGT GACTCCAGCA CACAAACTCA TTCTGTGACA 
GATCTTCAAG AAACCTCCCC TCTTGGACCT ACAGATGGCG ATGCTGGCGT CGATTTAGGA 
CGTTGTTCTG TTGTTCACAG ACTCAGCATC ACTGTAGAAG GTTGTGTGAT GGCAGATAAC 
CAGGGTTTGG ATTCTACCTC GTTTGCTACT GAAAGTGTTT TAAACCCAGA CGCAGGTCTT 
CCCACCCCTC CTTATAGTCC GTCTCACTCC CCTCTTGTTG GTAAACAGTG TCGTGCCCTT 
CACCATTCTG AGCCAATTCT AGACAGAGAG GTTATGGCTT TCTCATCTAC CTGCACTGCT 
TCTGTACCAC AGCGGAGCAG TTTTAAATCA GACCTCGAGC AAGCTCAAAG TCTTCCACCA 
GATGAAATCG AGATCCTGTG CGGAGACGAG GCGGAAAAAC TCCCGGAAAG ATCTTGCAGG 
ATGGAGAGCG GAGAGGTGGA TAACCGTAGA GCGGAGCACA GCAGGACGCT TCCTGACCGG 
GTGGTCGCAC CGCTGCTGGC AGGGGGACCA GAGGTCAGGC TTCGCAGGTC TGAGATGAAG 
TTGTGGGGTC CAGACGCAGA TGGTGATGTG AAGGAGAAAA AGCGCAGCTC GTTGTTTTCA 
CCCCGTAAAA GCAGGAAGAA TGGAAATGCA GCAGCTGAGA GCGGGCGAGA GACAGGGAAG 
CACAAGTCCC TCTGGAAGAC TGTTTTTTCA GTGTATAAGA AGGATAAAAA GAGGAAGGAG 
GTGGCAGTGG TGGCGGAAAC ACTTCCTGCT GCTAATAATG GGAGCAAACG GAAAGTGTCG 
GGAATTAACA GAACCACAGA CCTGTGTTTC CGGAAGAATC CAAGTTTCTC TGAAGATACG 
GATATGTCCT GTCATGCTCT TCTTGAAAGA TGTCCTCTCC GAGCTCAGAG AGCAGGAACA 
GAGGAGGAAC TCAATGCCAG GCTGACACGT CGAGTGCAGA GAGCAGCGCG CAGACAGGCC 
AAGCAGGAGG AGCTGCGGAG GCTGCATAGA GCTCAGATAA TCCAGCGTCA GTTGGAGCAG 
GTGGAAGTGA AGCAGAGGCA GCTGGAGGAG AAAGGTGTGG CTGTGGAGAA AGCACTCAGA 
GGAGAAGCAG TTGAGCCTTT TGACGGTTCT CCCCGTAGAA GGCCTGTATA TCTCTGTCCT 
TGCTGTTCCT CTGAAGGTAA AACCGGCCCT GCTTCCTTTC TCACTCCTCT AGTGCAGATT 
TTAGCAATAC TTTACAATCC TTTTTACCTT TTCTTCCTCT CTGCTCATTC GTCCTGCCTC 
CTGATGTGTG CAGCCAAAGC TAAAGGAACC GAACATGATC ATTCGGTTTA TGTCTGTACA 
GTCTTTTTCC TGTACGTAGT GCAGGAGCCT CCACTTCACC AACTGAGAAT GGGAAAGAAA 
GACGATCCAA GCTTAATGCA TCAGTGGTTC AAACTGGTGC AGGAGAAGAA TGCTCTCGTG 
CGTTATGAAT CTGAGCTCAT GATATTCGCT CGTGAACTGG AGCTGGAGGA CAGACAGAGT 
CGCCTGCAGC AGGAGCTGAG AGAGCGCATG GCGGTGGACG ATCATCTAAA AGGAGAAGAG 
GAGCTGGCGG AGGAGAGGCG TATATTGAGC GAGATGCTGG ATGTGGTGGA GCAGAGGGAT 
GCACTGGTGG CCCTCCTGGA GGAGCAGAGA GTTCGAGAAA AAGAGGAGGA CAGTGACCTG 
GAGGCTGTGA TGCTGTCTAA GGGCTTCAGT CTGCATTGGG ACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( XM_017355276.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210765.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X3, mRNA.

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CloneID	ODa58546
Clone ID Related Accession (Same CDS sequence)	XM_017355276.1
Accession Version	XM_017355276.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5322bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGCTCTG AGTCAGATCT AGAGGAGGAG GAGCTTGTCT GGAAGAAGGG GGAGGAGCTA 
CATGCCAGGA CCAATGGAGA GAGGAAGCTT GACCTAGAGG AGGAGCTGAA GGAAGAAGAG 
GGAGGGGAGA AGCTGGAAAA ACAAGAGGGG GAGGAAGAAG GAGAAGTTTC AGAAGAAGAG 
CAAGACGAGG GAGATTCTAG TGATGAGGGT GAGTACTGCC CTTGGGAGAG GGAACGACTC 
TCAGGGGTGT GGCTTGAGGA AGAGGAGGCG GGGTTTGTTA AAGAATCTTA TGAAGGATGC 
AGCGACGACC CTGATATAGA CGTCAGCTCA CTCAGTGAGT CCAAGCTATG TGACCAACGG 
GAACAGGAGG AGTCTGTTCC TTTCCACCAA TCCCCTGCCT CTACAGAATC TCCAGTCTCA 
ATGAAACCTT CAGAATCAGA CCTCACACCT GATCCGTCAA CAACCCCCGA GAGCTCGCCT 
GCCAAGAGGT CAGAGGTGGT GGAGGAGTTC TGGATGAAGA GTGCAGAGAT CCGAAAGAGT 
TTAGGCCTGA CTCCGCTTTC TAGAGAATTG CAGCCAAAAC ATATAGCAGC TTCAACCCAG 
ACATCTAATG TTAAAGAAAG CTTTTACACC TCAGTCACAT ATAACACCGC TGCTTTGGAT 
TCTCCAAACC AAAGTGCTAG AATCTGTGAC TCCAGCACAC AAACTCATTC TGTGACAGAT 
CTTCAAGAAA CCTCCCCTCT TGGACCTACA GATGGCGATG CTGGCGTCGA TTTAGGACGT 
TGTTCTGTTG TTCACAGACT CAGCATCACT GTAGAAGGTT GTGTGATGGC AGATAACCAG 
GGTTTGGATT CTACCTCGTT TGCTACTGAA AGTGTTTTAA ACCCAGACGC AGGTCTTCCC 
ACCCCTCCTT ATAGTCCGTC TCACTCCCCT CTTGTTGGTA AACAGTGTCG TGCCCTTCAC 
CATTCTGAGC CAATTCTAGA CAGAGAGGTT ATGGCTTTCT CATCTACCTG CACTGCTTCT 
GTACCACAGC GGAGCAGTTT TAAATCAGAC CTCGAGCAAG CTCAAAGTCT TCCACCAGAT 
GAAATCGAGA TCCTGTGCGG AGACGAGGCG GAAAAACTCC CGGAAAGATC TTGCAGGATG 
GAGAGCGGAG AGGTGGATAA CCGTAGAGCG GAGCACAGCA GGACGCTTCC TGACCGGGTG 
GTCGCACCGC TGCTGGCAGG GGGACCAGAG GTCAGGCTTC GCAGGTCTGA GATGAAGTTG 
TGGGGTCCAG ACGCAGATGG TGATGTGAAG GAGAAAAAGC GCAGCTCGTT GTTTTCACCC 
CGTAAAAGCA GGAAGAATGG AAATGCAGCA GCTGAGAGCG GGCGAGAGAC AGGGAAGCAC 
AAGTCCCTCT GGAAGACTGT TTTTTCAGTG TATAAGAAGG ATAAAAAGAG GAAGGAGGTG 
GCAGTGGTGG CGGAAACACT TCCTGCTGCT AATAATGGGA GCAAACGGAA AGTGTCGGGA 
ATTAACAGAA CCACAGACCT GTGTTTCCGG AAGAATCCAA GTTTCTCTGA AGATACGGAT 
ATGTCCTGTC ATGCTCTTCT TGAAAGATGT CCTCTCCGAG CTCAGAGAGC AGGAACAGAG 
GAGGAACTCA ATGCCAGGCT GACACGTCGA GTGCAGAGAG CAGCGCGCAG ACAGGCCAAG 
CAGGAGGAGC TGCGGAGGCT GCATAGAGCT CAGATAATCC AGCGTCAGTT GGAGCAGGTG 
GAAGTGAAGC AGAGGCAGCT GGAGGAGAAA GGTGTGGCTG TGGAGAAAGC ACTCAGAGGA 
GAAGCAGTTG AGCCTTTTGA CGGTTCTCCC CGTAGAAGGC CTGTATATCT CTGTCCTTGC 
TGTTCCTCTG AAGGTAAAAC CGGCCCTGCT TCCTTTCTCA CTCCTCTAGT GCAGATTTTA 
GCAATACTTT ACAATCCTTT TTACCTTTTC TTCCTCTCTG CTCATTCGTC CTGCCTCCTG 
ATGTGTGCAG CCAAAGCTAA AGGAACCGAA CATGATCATT CGGTTTATGT CTGTACAGTC 
TTTTTCCTGT ACGTAGTGCA GGAGCCTCCA CTTCACCAAC TGAGAATGGG AAAGAAAGAC 
GATCCAAGCT TAATGCATCA GTGGTTCAAA CTGGTGCAGG AGAAGAATGC TCTCGTGCGT 
TATGAATCTG AGCTCATGAT ATTCGCTCGT GAACTGGAGC TGGAGGACAG ACAGAGTCGC 
CTGCAGCAGG AGCTGAGAGA GCGCATGGCG GTGGACGATC ATCTAAAAGG AGAAGAGGAG 
CTGGCGGAGG AGAGGCGTAT ATTGAGCGAG ATGCTGGATG TGGTGGAGCA GAGGGATGCA 
CTGGTGGCCC TCCTGGAGGA GCAGAGAGTT CGAGAAAAAG AGGAGGACAG TGACCTGGAG 
GCTGTGATGC TGTCTAAGGG CTTCAGTCTG CATTGGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210765.1
CDS	296..5617
Translation	MWDGQSEMCQAHVLFDSFVQAATCKETLRAFQDLCEELNLNPGGQPQFYHTLRSRLHYWKAKALWAKLDKRACQKEYMRGHACTSTTCLIIGAGPCGLRTAIELGFLGARVVLVEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRKVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTAIRRQNYIHMYTGGVSSLALQIANQIQSQQAQAPKLLHRRESGSQKDLPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSESDLEEEELVWKKGEELHARTNGERKLDLEEELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDEGEYCPWERERLSGVWLEEEEAGFVKESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNGSKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEAVEPFDGSPRRRPVYLCPCCSSEGKTGPASFLTPLVQILAILYNPFYLFFLSAHSSCLLMCAAKAKGTEHDHSVYVCTVFFLYVVQEPPLHQLRMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	XM_017355276.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355276.1

ORF Insert Sequence:

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGCTCTG AGTCAGATCT AGAGGAGGAG GAGCTTGTCT GGAAGAAGGG GGAGGAGCTA 
CATGCCAGGA CCAATGGAGA GAGGAAGCTT GACCTAGAGG AGGAGCTGAA GGAAGAAGAG 
GGAGGGGAGA AGCTGGAAAA ACAAGAGGGG GAGGAAGAAG GAGAAGTTTC AGAAGAAGAG 
CAAGACGAGG GAGATTCTAG TGATGAGGGT GAGTACTGCC CTTGGGAGAG GGAACGACTC 
TCAGGGGTGT GGCTTGAGGA AGAGGAGGCG GGGTTTGTTA AAGAATCTTA TGAAGGATGC 
AGCGACGACC CTGATATAGA CGTCAGCTCA CTCAGTGAGT CCAAGCTATG TGACCAACGG 
GAACAGGAGG AGTCTGTTCC TTTCCACCAA TCCCCTGCCT CTACAGAATC TCCAGTCTCA 
ATGAAACCTT CAGAATCAGA CCTCACACCT GATCCGTCAA CAACCCCCGA GAGCTCGCCT 
GCCAAGAGGT CAGAGGTGGT GGAGGAGTTC TGGATGAAGA GTGCAGAGAT CCGAAAGAGT 
TTAGGCCTGA CTCCGCTTTC TAGAGAATTG CAGCCAAAAC ATATAGCAGC TTCAACCCAG 
ACATCTAATG TTAAAGAAAG CTTTTACACC TCAGTCACAT ATAACACCGC TGCTTTGGAT 
TCTCCAAACC AAAGTGCTAG AATCTGTGAC TCCAGCACAC AAACTCATTC TGTGACAGAT 
CTTCAAGAAA CCTCCCCTCT TGGACCTACA GATGGCGATG CTGGCGTCGA TTTAGGACGT 
TGTTCTGTTG TTCACAGACT CAGCATCACT GTAGAAGGTT GTGTGATGGC AGATAACCAG 
GGTTTGGATT CTACCTCGTT TGCTACTGAA AGTGTTTTAA ACCCAGACGC AGGTCTTCCC 
ACCCCTCCTT ATAGTCCGTC TCACTCCCCT CTTGTTGGTA AACAGTGTCG TGCCCTTCAC 
CATTCTGAGC CAATTCTAGA CAGAGAGGTT ATGGCTTTCT CATCTACCTG CACTGCTTCT 
GTACCACAGC GGAGCAGTTT TAAATCAGAC CTCGAGCAAG CTCAAAGTCT TCCACCAGAT 
GAAATCGAGA TCCTGTGCGG AGACGAGGCG GAAAAACTCC CGGAAAGATC TTGCAGGATG 
GAGAGCGGAG AGGTGGATAA CCGTAGAGCG GAGCACAGCA GGACGCTTCC TGACCGGGTG 
GTCGCACCGC TGCTGGCAGG GGGACCAGAG GTCAGGCTTC GCAGGTCTGA GATGAAGTTG 
TGGGGTCCAG ACGCAGATGG TGATGTGAAG GAGAAAAAGC GCAGCTCGTT GTTTTCACCC 
CGTAAAAGCA GGAAGAATGG AAATGCAGCA GCTGAGAGCG GGCGAGAGAC AGGGAAGCAC 
AAGTCCCTCT GGAAGACTGT TTTTTCAGTG TATAAGAAGG ATAAAAAGAG GAAGGAGGTG 
GCAGTGGTGG CGGAAACACT TCCTGCTGCT AATAATGGGA GCAAACGGAA AGTGTCGGGA 
ATTAACAGAA CCACAGACCT GTGTTTCCGG AAGAATCCAA GTTTCTCTGA AGATACGGAT 
ATGTCCTGTC ATGCTCTTCT TGAAAGATGT CCTCTCCGAG CTCAGAGAGC AGGAACAGAG 
GAGGAACTCA ATGCCAGGCT GACACGTCGA GTGCAGAGAG CAGCGCGCAG ACAGGCCAAG 
CAGGAGGAGC TGCGGAGGCT GCATAGAGCT CAGATAATCC AGCGTCAGTT GGAGCAGGTG 
GAAGTGAAGC AGAGGCAGCT GGAGGAGAAA GGTGTGGCTG TGGAGAAAGC ACTCAGAGGA 
GAAGCAGTTG AGCCTTTTGA CGGTTCTCCC CGTAGAAGGC CTGTATATCT CTGTCCTTGC 
TGTTCCTCTG AAGGTAAAAC CGGCCCTGCT TCCTTTCTCA CTCCTCTAGT GCAGATTTTA 
GCAATACTTT ACAATCCTTT TTACCTTTTC TTCCTCTCTG CTCATTCGTC CTGCCTCCTG 
ATGTGTGCAG CCAAAGCTAA AGGAACCGAA CATGATCATT CGGTTTATGT CTGTACAGTC 
TTTTTCCTGT ACGTAGTGCA GGAGCCTCCA CTTCACCAAC TGAGAATGGG AAAGAAAGAC 
GATCCAAGCT TAATGCATCA GTGGTTCAAA CTGGTGCAGG AGAAGAATGC TCTCGTGCGT 
TATGAATCTG AGCTCATGAT ATTCGCTCGT GAACTGGAGC TGGAGGACAG ACAGAGTCGC 
CTGCAGCAGG AGCTGAGAGA GCGCATGGCG GTGGACGATC ATCTAAAAGG AGAAGAGGAG 
CTGGCGGAGG AGAGGCGTAT ATTGAGCGAG ATGCTGGATG TGGTGGAGCA GAGGGATGCA 
CTGGTGGCCC TCCTGGAGGA GCAGAGAGTT CGAGAAAAAG AGGAGGACAG TGACCTGGAG 
GCTGTGATGC TGTCTAAGGG CTTCAGTCTG CATTGGGACT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( XM_017355277.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210766.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X4, mRNA.

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CloneID	ODa58547
Clone ID Related Accession (Same CDS sequence)	XM_017355277.1
Accession Version	XM_017355277.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGAGGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAA TCTTATGAAG GATGCAGCGA CGACCCTGAT 
ATAGACGTCA GCTCACTCAG TGAGTCCAAG CTATGTGACC AACGGGAACA GGAGGAGTCT 
GTTCCTTTCC ACCAATCCCC TGCCTCTACA GAATCTCCAG TCTCAATGAA ACCTTCAGAA 
TCAGACCTCA CACCTGATCC GTCAACAACC CCCGAGAGCT CGCCTGCCAA GAGGTCAGAG 
GTGGTGGAGG AGTTCTGGAT GAAGAGTGCA GAGATCCGAA AGAGTTTAGG CCTGACTCCG 
CTTTCTAGAG AATTGCAGCC AAAACATATA GCAGCTTCAA CCCAGACATC TAATGTTAAA 
GAAAGCTTTT ACACCTCAGT CACATATAAC ACCGCTGCTT TGGATTCTCC AAACCAAAGT 
GCTAGAATCT GTGACTCCAG CACACAAACT CATTCTGTGA CAGATCTTCA AGAAACCTCC 
CCTCTTGGAC CTACAGATGG CGATGCTGGC GTCGATTTAG GACGTTGTTC TGTTGTTCAC 
AGACTCAGCA TCACTGTAGA AGGTTGTGTG ATGGCAGATA ACCAGGGTTT GGATTCTACC 
TCGTTTGCTA CTGAAAGTGT TTTAAACCCA GACGCAGGTC TTCCCACCCC TCCTTATAGT 
CCGTCTCACT CCCCTCTTGT TGGTAAACAG TGTCGTGCCC TTCACCATTC TGAGCCAATT 
CTAGACAGAG AGGTTATGGC TTTCTCATCT ACCTGCACTG CTTCTGTACC ACAGCGGAGC 
AGTTTTAAAT CAGACCTCGA GCAAGCTCAA AGTCTTCCAC CAGATGAAAT CGAGATCCTG 
TGCGGAGACG AGGCGGAAAA ACTCCCGGAA AGATCTTGCA GGATGGAGAG CGGAGAGGTG 
GATAACCGTA GAGCGGAGCA CAGCAGGACG CTTCCTGACC GGGTGGTCGC ACCGCTGCTG 
GCAGGGGGAC CAGAGGTCAG GCTTCGCAGG TCTGAGATGA AGTTGTGGGG TCCAGACGCA 
GATGGTGATG TGAAGGAGAA AAAGCGCAGC TCGTTGTTTT CACCCCGTAA AAGCAGGAAG 
AATGGAAATG CAGCAGCTGA GAGCGGGCGA GAGACAGGGA AGCACAAGTC CCTCTGGAAG 
ACTGTTTTTT CAGTGTATAA GAAGGATAAA AAGAGGAAGG AGGTGGCAGT GGTGGCGGAA 
ACACTTCCTG CTGCTAATAA TGGGAGCAAA CGGAAAGTGT CGGGAATTAA CAGAACCACA 
GACCTGTGTT TCCGGAAGAA TCCAAGTTTC TCTGAAGATA CGGATATGTC CTGTCATGCT 
CTTCTTGAAA GATGTCCTCT CCGAGCTCAG AGAGCAGGAA CAGAGGAGGA ACTCAATGCC 
AGGCTGACAC GTCGAGTGCA GAGAGCAGCG CGCAGACAGG CCAAGCAGGA GGAGCTGCGG 
AGGCTGCATA GAGCTCAGAT AATCCAGCGT CAGTTGGAGC AGGTGGAAGT GAAGCAGAGG 
CAGCTGGAGG AGAAAGGTGT GGCTGTGGAG AAAGCACTCA GAGGAGAAGC AGTTGAGCCT 
TTTGACGGTT CTCCCCGTAG AAGGCCTGTA TATCTCTGTC CTTGCTGTTC CTCTGAAGGT 
AAAACCGGCC CTGCTTCCTT TCTCACTCCT CTAGTGCAGA TTTTAGCAAT ACTTTACAAT 
CCTTTTTACC TTTTCTTCCT CTCTGCTCAT TCGTCCTGCC TCCTGATGTG TGCAGCCAAA 
GCTAAAGGAA CCGAACATGA TCATTCGGTT TATGTCTGTA CAGTCTTTTT CCTGTACGTA 
GTGCAGGAGC CTCCACTTCA CCAACTGAGA ATGGGAAAGA AAGACGATCC AAGCTTAATG 
CATCAGTGGT TCAAACTGGT GCAGGAGAAG AATGCTCTCG TGCGTTATGA ATCTGAGCTC 
ATGATATTCG CTCGTGAACT GGAGCTGGAG GACAGACAGA GTCGCCTGCA GCAGGAGCTG 
AGAGAGCGCA TGGCGGTGGA CGATCATCTA AAAGGAGAAG AGGAGCTGGC GGAGGAGAGG 
CGTATATTGA GCGAGATGCT GGATGTGGTG GAGCAGAGGG ATGCACTGGT GGCCCTCCTG 
GAGGAGCAGA GAGTTCGAGA AAAAGAGGAG GACAGTGACC TGGAGGCTGT GATGCTGTCT 
AAGGGCTTCA GTCTGCATTG GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210766.1
CDS	296..5542
Translation	MWDGQSEMCQAHVLFDSFVQAATCKETLRAFQDLCEELNLNPGGQPQFYHTLRSRLHYWKAKALWAKLDKRACQKEYMRGHACTSTTCLIIGAGPCGLRTAIELGFLGARVVLVEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRKVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTAIRRQNYIHMYTGGVSSLALQIANQIQSQQAQAPKLLHRRESGSQKDLPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSSESDLEEEELVWKKGEELHARTNGERKLDLEEELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNGSKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEAVEPFDGSPRRRPVYLCPCCSSEGKTGPASFLTPLVQILAILYNPFYLFFLSAHSSCLLMCAAKAKGTEHDHSVYVCTVFFLYVVQEPPLHQLRMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	XM_017355277.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355277.1

ORF Insert Sequence:

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGAGGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAA TCTTATGAAG GATGCAGCGA CGACCCTGAT 
ATAGACGTCA GCTCACTCAG TGAGTCCAAG CTATGTGACC AACGGGAACA GGAGGAGTCT 
GTTCCTTTCC ACCAATCCCC TGCCTCTACA GAATCTCCAG TCTCAATGAA ACCTTCAGAA 
TCAGACCTCA CACCTGATCC GTCAACAACC CCCGAGAGCT CGCCTGCCAA GAGGTCAGAG 
GTGGTGGAGG AGTTCTGGAT GAAGAGTGCA GAGATCCGAA AGAGTTTAGG CCTGACTCCG 
CTTTCTAGAG AATTGCAGCC AAAACATATA GCAGCTTCAA CCCAGACATC TAATGTTAAA 
GAAAGCTTTT ACACCTCAGT CACATATAAC ACCGCTGCTT TGGATTCTCC AAACCAAAGT 
GCTAGAATCT GTGACTCCAG CACACAAACT CATTCTGTGA CAGATCTTCA AGAAACCTCC 
CCTCTTGGAC CTACAGATGG CGATGCTGGC GTCGATTTAG GACGTTGTTC TGTTGTTCAC 
AGACTCAGCA TCACTGTAGA AGGTTGTGTG ATGGCAGATA ACCAGGGTTT GGATTCTACC 
TCGTTTGCTA CTGAAAGTGT TTTAAACCCA GACGCAGGTC TTCCCACCCC TCCTTATAGT 
CCGTCTCACT CCCCTCTTGT TGGTAAACAG TGTCGTGCCC TTCACCATTC TGAGCCAATT 
CTAGACAGAG AGGTTATGGC TTTCTCATCT ACCTGCACTG CTTCTGTACC ACAGCGGAGC 
AGTTTTAAAT CAGACCTCGA GCAAGCTCAA AGTCTTCCAC CAGATGAAAT CGAGATCCTG 
TGCGGAGACG AGGCGGAAAA ACTCCCGGAA AGATCTTGCA GGATGGAGAG CGGAGAGGTG 
GATAACCGTA GAGCGGAGCA CAGCAGGACG CTTCCTGACC GGGTGGTCGC ACCGCTGCTG 
GCAGGGGGAC CAGAGGTCAG GCTTCGCAGG TCTGAGATGA AGTTGTGGGG TCCAGACGCA 
GATGGTGATG TGAAGGAGAA AAAGCGCAGC TCGTTGTTTT CACCCCGTAA AAGCAGGAAG 
AATGGAAATG CAGCAGCTGA GAGCGGGCGA GAGACAGGGA AGCACAAGTC CCTCTGGAAG 
ACTGTTTTTT CAGTGTATAA GAAGGATAAA AAGAGGAAGG AGGTGGCAGT GGTGGCGGAA 
ACACTTCCTG CTGCTAATAA TGGGAGCAAA CGGAAAGTGT CGGGAATTAA CAGAACCACA 
GACCTGTGTT TCCGGAAGAA TCCAAGTTTC TCTGAAGATA CGGATATGTC CTGTCATGCT 
CTTCTTGAAA GATGTCCTCT CCGAGCTCAG AGAGCAGGAA CAGAGGAGGA ACTCAATGCC 
AGGCTGACAC GTCGAGTGCA GAGAGCAGCG CGCAGACAGG CCAAGCAGGA GGAGCTGCGG 
AGGCTGCATA GAGCTCAGAT AATCCAGCGT CAGTTGGAGC AGGTGGAAGT GAAGCAGAGG 
CAGCTGGAGG AGAAAGGTGT GGCTGTGGAG AAAGCACTCA GAGGAGAAGC AGTTGAGCCT 
TTTGACGGTT CTCCCCGTAG AAGGCCTGTA TATCTCTGTC CTTGCTGTTC CTCTGAAGGT 
AAAACCGGCC CTGCTTCCTT TCTCACTCCT CTAGTGCAGA TTTTAGCAAT ACTTTACAAT 
CCTTTTTACC TTTTCTTCCT CTCTGCTCAT TCGTCCTGCC TCCTGATGTG TGCAGCCAAA 
GCTAAAGGAA CCGAACATGA TCATTCGGTT TATGTCTGTA CAGTCTTTTT CCTGTACGTA 
GTGCAGGAGC CTCCACTTCA CCAACTGAGA ATGGGAAAGA AAGACGATCC AAGCTTAATG 
CATCAGTGGT TCAAACTGGT GCAGGAGAAG AATGCTCTCG TGCGTTATGA ATCTGAGCTC 
ATGATATTCG CTCGTGAACT GGAGCTGGAG GACAGACAGA GTCGCCTGCA GCAGGAGCTG 
AGAGAGCGCA TGGCGGTGGA CGATCATCTA AAAGGAGAAG AGGAGCTGGC GGAGGAGAGG 
CGTATATTGA GCGAGATGCT GGATGTGGTG GAGCAGAGGG ATGCACTGGT GGCCCTCCTG 
GAGGAGCAGA GAGTTCGAGA AAAAGAGGAG GACAGTGACC TGGAGGCTGT GATGCTGTCT 
AAGGGCTTCA GTCTGCATTG GGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( XM_017355278.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210767.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X5, mRNA.

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CloneID	ODa58548
Clone ID Related Accession (Same CDS sequence)	XM_017355278.1
Accession Version	XM_017355278.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
GGTTCTCAGA AGGATCTTCC AGTAAATGTC GGCAGTAGTG ATGTGTGTTA TTTCTGTGGC 
CGTCGCGTTT ATGTCATGGA GAGGCTGAGC GCAGAGGGAA AGTTCTTCCA CAGGAGCTGC 
TTCCAGTGCG ACCACTGCAG CTCCACGATA CGCCTCTCCA ACTACACTTA TGACCAGCTA 
CATGGCAAGT TTTACTGTAA GCATCACTTC AGCTTCAGAC TGGCAAGTGT GGCTCAGAGA 
AAGAGACCGG CCCCACCGGT GGCCCCTCGG CCTGCTCAGG CTTCCCTGGC TGCATCCTCG 
GCCTCTACCT CTTTGTCCTC TTTGGGCTCA GTGGGCACAG CCACACCAGA CAGCTGGTCC 
TCCAGCACCC ACACAGACAT GGCATCCAGC TTGGCCAAGA GACTGTGCGG CACACCAGAA 
CGCATTGAGC TTGAGAATTA CAAACCCTCC CCACAGAAAC AAGACAGCCC CCTGCAGGAG 
GTACCAGAGG AAACCCTCGC ACAGCACAAT CTCAGCGCTA GCCTGCAGGA GAAGAATGCT 
GAAGAGCAGT CCAGTAGCTC TGAGTCAGAT CTAGAGGAGG AGGAGCTTGT CTGGAAGAAG 
GGGGAGGAGC TACATGCCAG GACCAATGGA GAGAGGAAGC TTGACCTAGA GGAGGAGCTG 
AAGGAAGAAG AGGGAGGGGA GAAGCTGGAA AAACAAGAGG GGGAGGAAGA AGGAGAAGTT 
TCAGAAGAAG AGCAAGACGA GGGAGATTCT AGTGATGAGG GTGAGTACTG CCCTTGGGAG 
AGGGAACGAC TCTCAGGGGT GTGGCTTGAG GAAGAGGAGG CGGGGTTTGT TAAAGAATCT 
TATGAAGGAT GCAGCGACGA CCCTGATATA GACGTCAGCT CACTCAGTGA GTCCAAGCTA 
TGTGACCAAC GGGAACAGGA GGAGTCTGTT CCTTTCCACC AATCCCCTGC CTCTACAGAA 
TCTCCAGTCT CAATGAAACC TTCAGAATCA GACCTCACAC CTGATCCGTC AACAACCCCC 
GAGAGCTCGC CTGCCAAGAG GTCAGAGGTG GTGGAGGAGT TCTGGATGAA GAGTGCAGAG 
ATCCGAAAGA GTTTAGGCCT GACTCCGCTT TCTAGAGAAT TGCAGCCAAA ACATATAGCA 
GCTTCAACCC AGACATCTAA TGTTAAAGAA AGCTTTTACA CCTCAGTCAC ATATAACACC 
GCTGCTTTGG ATTCTCCAAA CCAAAGTGCT AGAATCTGTG ACTCCAGCAC ACAAACTCAT 
TCTGTGACAG ATCTTCAAGA AACCTCCCCT CTTGGACCTA CAGATGGCGA TGCTGGCGTC 
GATTTAGGAC GTTGTTCTGT TGTTCACAGA CTCAGCATCA CTGTAGAAGG TTGTGTGATG 
GCAGATAACC AGGGTTTGGA TTCTACCTCG TTTGCTACTG AAAGTGTTTT AAACCCAGAC 
GCAGGTCTTC CCACCCCTCC TTATAGTCCG TCTCACTCCC CTCTTGTTGG TAAACAGTGT 
CGTGCCCTTC ACCATTCTGA GCCAATTCTA GACAGAGAGG TTATGGCTTT CTCATCTACC 
TGCACTGCTT CTGTACCACA GCGGAGCAGT TTTAAATCAG ACCTCGAGCA AGCTCAAAGT 
CTTCCACCAG ATGAAATCGA GATCCTGTGC GGAGACGAGG CGGAAAAACT CCCGGAAAGA 
TCTTGCAGGA TGGAGAGCGG AGAGGTGGAT AACCGTAGAG CGGAGCACAG CAGGACGCTT 
CCTGACCGGG TGGTCGCACC GCTGCTGGCA GGGGGACCAG AGGTCAGGCT TCGCAGGTCT 
GAGATGAAGT TGTGGGGTCC AGACGCAGAT GGTGATGTGA AGGAGAAAAA GCGCAGCTCG 
TTGTTTTCAC CCCGTAAAAG CAGGAAGAAT GGAAATGCAG CAGCTGAGAG CGGGCGAGAG 
ACAGGGAAGC ACAAGTCCCT CTGGAAGACT GTTTTTTCAG TGTATAAGAA GGATAAAAAG 
AGGAAGGAGG TGGCAGTGGT GGCGGAAACA CTTCCTGCTG CTAATAATGG GAGCAAACGG 
AAAGTGTCGG GAATTAACAG AACCACAGAC CTGTGTTTCC GGAAGAATCC AAGTTTCTCT 
GAAGATACGG ATATGTCCTG TCATGCTCTT CTTGAAAGAT GTCCTCTCCG AGCTCAGAGA 
GCAGGAACAG AGGAGGAACT CAATGCCAGG CTGACACGTC GAGTGCAGAG AGCAGCGCGC 
AGACAGGCCA AGCAGGAGGA GCTGCGGAGG CTGCATAGAG CTCAGATAAT CCAGCGTCAG 
TTGGAGCAGG TGGAAGTGAA GCAGAGGCAG CTGGAGGAGA AAGGTGTGGC TGTGGAGAAA 
GCACTCAGAG GAGAAGCAGT TGAGCCTTTT GACGGTTCTC CCCGTAGAAG GCCTGTATAT 
CTCTGTCCTT GCTGTTCCTC TGAAGGTAAA ACCGGCCCTG CTTCCTTTCT CACTCCTCTA 
GTGCAGATTT TAGCAATACT TTACAATCCT TTTTACCTTT TCTTCCTCTC TGCTCATTCG 
TCCTGCCTCC TGATGTGTGC AGCCAAAGCT AAAGGAACCG AACATGATCA TTCGGTTTAT 
GTCTGTACAG TCTTTTTCCT GTACGTAGTG CAGGAGCCTC CACTTCACCA ACTGAGAATG 
GGAAAGAAAG ACGATCCAAG CTTAATGCAT CAGTGGTTCA AACTGGTGCA GGAGAAGAAT 
GCTCTCGTGC GTTATGAATC TGAGCTCATG ATATTCGCTC GTGAACTGGA GCTGGAGGAC 
AGACAGAGTC GCCTGCAGCA GGAGCTGAGA GAGCGCATGG CGGTGGACGA TCATCTAAAA 
GGAGAAGAGG AGCTGGCGGA GGAGAGGCGT ATATTGAGCG AGATGCTGGA TGTGGTGGAG 
CAGAGGGATG CACTGGTGGC CCTCCTGGAG GAGCAGAGAG TTCGAGAAAA AGAGGAGGAC 
AGTGACCTGG AGGCTGTGAT GCTGTCTAAG GGCTTCAGTC TGCATTGGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210767.1
CDS	296..5509
Translation	MWDGQSEMCQAHVLFDSFVQAATCKETLRAFQDLCEELNLNPGGQPQFYHTLRSRLHYWKAKALWAKLDKRACQKEYMRGHACTSTTCLIIGAGPCGLRTAIELGFLGARVVLVEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRKVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTAIRRQGSQKDLPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSSESDLEEEELVWKKGEELHARTNGERKLDLEEELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDEGEYCPWERERLSGVWLEEEEAGFVKESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNGSKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEAVEPFDGSPRRRPVYLCPCCSSEGKTGPASFLTPLVQILAILYNPFYLFFLSAHSSCLLMCAAKAKGTEHDHSVYVCTVFFLYVVQEPPLHQLRMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	XM_017355278.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355278.1

ORF Insert Sequence:

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
GGTTCTCAGA AGGATCTTCC AGTAAATGTC GGCAGTAGTG ATGTGTGTTA TTTCTGTGGC 
CGTCGCGTTT ATGTCATGGA GAGGCTGAGC GCAGAGGGAA AGTTCTTCCA CAGGAGCTGC 
TTCCAGTGCG ACCACTGCAG CTCCACGATA CGCCTCTCCA ACTACACTTA TGACCAGCTA 
CATGGCAAGT TTTACTGTAA GCATCACTTC AGCTTCAGAC TGGCAAGTGT GGCTCAGAGA 
AAGAGACCGG CCCCACCGGT GGCCCCTCGG CCTGCTCAGG CTTCCCTGGC TGCATCCTCG 
GCCTCTACCT CTTTGTCCTC TTTGGGCTCA GTGGGCACAG CCACACCAGA CAGCTGGTCC 
TCCAGCACCC ACACAGACAT GGCATCCAGC TTGGCCAAGA GACTGTGCGG CACACCAGAA 
CGCATTGAGC TTGAGAATTA CAAACCCTCC CCACAGAAAC AAGACAGCCC CCTGCAGGAG 
GTACCAGAGG AAACCCTCGC ACAGCACAAT CTCAGCGCTA GCCTGCAGGA GAAGAATGCT 
GAAGAGCAGT CCAGTAGCTC TGAGTCAGAT CTAGAGGAGG AGGAGCTTGT CTGGAAGAAG 
GGGGAGGAGC TACATGCCAG GACCAATGGA GAGAGGAAGC TTGACCTAGA GGAGGAGCTG 
AAGGAAGAAG AGGGAGGGGA GAAGCTGGAA AAACAAGAGG GGGAGGAAGA AGGAGAAGTT 
TCAGAAGAAG AGCAAGACGA GGGAGATTCT AGTGATGAGG GTGAGTACTG CCCTTGGGAG 
AGGGAACGAC TCTCAGGGGT GTGGCTTGAG GAAGAGGAGG CGGGGTTTGT TAAAGAATCT 
TATGAAGGAT GCAGCGACGA CCCTGATATA GACGTCAGCT CACTCAGTGA GTCCAAGCTA 
TGTGACCAAC GGGAACAGGA GGAGTCTGTT CCTTTCCACC AATCCCCTGC CTCTACAGAA 
TCTCCAGTCT CAATGAAACC TTCAGAATCA GACCTCACAC CTGATCCGTC AACAACCCCC 
GAGAGCTCGC CTGCCAAGAG GTCAGAGGTG GTGGAGGAGT TCTGGATGAA GAGTGCAGAG 
ATCCGAAAGA GTTTAGGCCT GACTCCGCTT TCTAGAGAAT TGCAGCCAAA ACATATAGCA 
GCTTCAACCC AGACATCTAA TGTTAAAGAA AGCTTTTACA CCTCAGTCAC ATATAACACC 
GCTGCTTTGG ATTCTCCAAA CCAAAGTGCT AGAATCTGTG ACTCCAGCAC ACAAACTCAT 
TCTGTGACAG ATCTTCAAGA AACCTCCCCT CTTGGACCTA CAGATGGCGA TGCTGGCGTC 
GATTTAGGAC GTTGTTCTGT TGTTCACAGA CTCAGCATCA CTGTAGAAGG TTGTGTGATG 
GCAGATAACC AGGGTTTGGA TTCTACCTCG TTTGCTACTG AAAGTGTTTT AAACCCAGAC 
GCAGGTCTTC CCACCCCTCC TTATAGTCCG TCTCACTCCC CTCTTGTTGG TAAACAGTGT 
CGTGCCCTTC ACCATTCTGA GCCAATTCTA GACAGAGAGG TTATGGCTTT CTCATCTACC 
TGCACTGCTT CTGTACCACA GCGGAGCAGT TTTAAATCAG ACCTCGAGCA AGCTCAAAGT 
CTTCCACCAG ATGAAATCGA GATCCTGTGC GGAGACGAGG CGGAAAAACT CCCGGAAAGA 
TCTTGCAGGA TGGAGAGCGG AGAGGTGGAT AACCGTAGAG CGGAGCACAG CAGGACGCTT 
CCTGACCGGG TGGTCGCACC GCTGCTGGCA GGGGGACCAG AGGTCAGGCT TCGCAGGTCT 
GAGATGAAGT TGTGGGGTCC AGACGCAGAT GGTGATGTGA AGGAGAAAAA GCGCAGCTCG 
TTGTTTTCAC CCCGTAAAAG CAGGAAGAAT GGAAATGCAG CAGCTGAGAG CGGGCGAGAG 
ACAGGGAAGC ACAAGTCCCT CTGGAAGACT GTTTTTTCAG TGTATAAGAA GGATAAAAAG 
AGGAAGGAGG TGGCAGTGGT GGCGGAAACA CTTCCTGCTG CTAATAATGG GAGCAAACGG 
AAAGTGTCGG GAATTAACAG AACCACAGAC CTGTGTTTCC GGAAGAATCC AAGTTTCTCT 
GAAGATACGG ATATGTCCTG TCATGCTCTT CTTGAAAGAT GTCCTCTCCG AGCTCAGAGA 
GCAGGAACAG AGGAGGAACT CAATGCCAGG CTGACACGTC GAGTGCAGAG AGCAGCGCGC 
AGACAGGCCA AGCAGGAGGA GCTGCGGAGG CTGCATAGAG CTCAGATAAT CCAGCGTCAG 
TTGGAGCAGG TGGAAGTGAA GCAGAGGCAG CTGGAGGAGA AAGGTGTGGC TGTGGAGAAA 
GCACTCAGAG GAGAAGCAGT TGAGCCTTTT GACGGTTCTC CCCGTAGAAG GCCTGTATAT 
CTCTGTCCTT GCTGTTCCTC TGAAGGTAAA ACCGGCCCTG CTTCCTTTCT CACTCCTCTA 
GTGCAGATTT TAGCAATACT TTACAATCCT TTTTACCTTT TCTTCCTCTC TGCTCATTCG 
TCCTGCCTCC TGATGTGTGC AGCCAAAGCT AAAGGAACCG AACATGATCA TTCGGTTTAT 
GTCTGTACAG TCTTTTTCCT GTACGTAGTG CAGGAGCCTC CACTTCACCA ACTGAGAATG 
GGAAAGAAAG ACGATCCAAG CTTAATGCAT CAGTGGTTCA AACTGGTGCA GGAGAAGAAT 
GCTCTCGTGC GTTATGAATC TGAGCTCATG ATATTCGCTC GTGAACTGGA GCTGGAGGAC 
AGACAGAGTC GCCTGCAGCA GGAGCTGAGA GAGCGCATGG CGGTGGACGA TCATCTAAAA 
GGAGAAGAGG AGCTGGCGGA GGAGAGGCGT ATATTGAGCG AGATGCTGGA TGTGGTGGAG 
CAGAGGGATG CACTGGTGGC CCTCCTGGAG GAGCAGAGAG TTCGAGAAAA AGAGGAGGAC 
AGTGACCTGG AGGCTGTGAT GCTGTCTAAG GGCTTCAGTC TGCATTGGGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( XM_017355279.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210768.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X1, mRNA.

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CloneID	ODa58549
Clone ID Related Accession (Same CDS sequence)	XM_017355279.2 , XM_017355279.1 , XM_021475214.1
Accession Version	XM_017355279.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017355279.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGAGGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAG GGTGAGTACT GCCCTTGGGA GAGGGAACGA 
CTCTCAGGGG TGTGGCTTGA GGAAGAGGAG GCGGGGTTTG TTAAAGAATC TTATGAAGGA 
TGCAGCGACG ACCCTGATAT AGACGTCAGC TCACTCAGTG AGTCCAAGCT ATGTGACCAA 
CGGGAACAGG AGGAGTCTGT TCCTTTCCAC CAATCCCCTG CCTCTACAGA ATCTCCAGTC 
TCAATGAAAC CTTCAGAATC AGACCTCACA CCTGATCCGT CAACAACCCC CGAGAGCTCG 
CCTGCCAAGA GGTCAGAGGT GGTGGAGGAG TTCTGGATGA AGAGTGCAGA GATCCGAAAG 
AGTTTAGGCC TGACTCCGCT TTCTAGAGAA TTGCAGCCAA AACATATAGC AGCTTCAACC 
CAGACATCTA ATGTTAAAGA AAGCTTTTAC ACCTCAGTCA CATATAACAC CGCTGCTTTG 
GATTCTCCAA ACCAAAGTGC TAGAATCTGT GACTCCAGCA CACAAACTCA TTCTGTGACA 
GATCTTCAAG AAACCTCCCC TCTTGGACCT ACAGATGGCG ATGCTGGCGT CGATTTAGGA 
CGTTGTTCTG TTGTTCACAG ACTCAGCATC ACTGTAGAAG GTTGTGTGAT GGCAGATAAC 
CAGGGTTTGG ATTCTACCTC GTTTGCTACT GAAAGTGTTT TAAACCCAGA CGCAGGTCTT 
CCCACCCCTC CTTATAGTCC GTCTCACTCC CCTCTTGTTG GTAAACAGTG TCGTGCCCTT 
CACCATTCTG AGCCAATTCT AGACAGAGAG GTTATGGCTT TCTCATCTAC CTGCACTGCT 
TCTGTACCAC AGCGGAGCAG TTTTAAATCA GACCTCGAGC AAGCTCAAAG TCTTCCACCA 
GATGAAATCG AGATCCTGTG CGGAGACGAG GCGGAAAAAC TCCCGGAAAG ATCTTGCAGG 
ATGGAGAGCG GAGAGGTGGA TAACCGTAGA GCGGAGCACA GCAGGACGCT TCCTGACCGG 
GTGGTCGCAC CGCTGCTGGC AGGGGGACCA GAGGTCAGGC TTCGCAGGTC TGAGATGAAG 
TTGTGGGGTC CAGACGCAGA TGGTGATGTG AAGGAGAAAA AGCGCAGCTC GTTGTTTTCA 
CCCCGTAAAA GCAGGAAGAA TGGAAATGCA GCAGCTGAGA GCGGGCGAGA GACAGGGAAG 
CACAAGTCCC TCTGGAAGAC TGTTTTTTCA GTGTATAAGA AGGATAAAAA GAGGAAGGAG 
GTGGCAGTGG TGGCGGAAAC ACTTCCTGCT GCTAATAATG GGAGCAAACG GAAAGTGTCG 
GGAATTAACA GAACCACAGA CCTGTGTTTC CGGAAGAATC CAAGTTTCTC TGAAGATACG 
GATATGTCCT GTCATGCTCT TCTTGAAAGA TGTCCTCTCC GAGCTCAGAG AGCAGGAACA 
GAGGAGGAAC TCAATGCCAG GCTGACACGT CGAGTGCAGA GAGCAGCGCG CAGACAGGCC 
AAGCAGGAGG AGCTGCGGAG GCTGCATAGA GCTCAGATAA TCCAGCGTCA GTTGGAGCAG 
GTGGAAGTGA AGCAGAGGCA GCTGGAGGAG AAAGGTGTGG CTGTGGAGAA AGCACTCAGA 
GGAGAAGCAG TTGAGCCTTT TGACGGTTCT CCCCGTAGAA GGCCTGTATA TCTCTGTCCT 
TGCTGTTCCT CTGAAGCCAA AGCTAAAGGA ACCGAACATG ATCATTCGGT TTATGTCTGT 
ACAGTCTTTT TCCTGTACGT AGTGCAGGAG CCTCCACTTC ACCAACTGAG AATGGGAAAG 
AAAGACGATC CAAGCTTAAT GCATCAGTGG TTCAAACTGG TGCAGGAGAA GAATGCTCTC 
GTGCGTTATG AATCTGAGCT CATGATATTC GCTCGTGAAC TGGAGCTGGA GGACAGACAG 
AGTCGCCTGC AGCAGGAGCT GAGAGAGCGC ATGGCGGTGG ACGATCATCT AAAAGGAGAA 
GAGGAGCTGG CGGAGGAGAG GCGTATATTG AGCGAGATGC TGGATGTGGT GGAGCAGAGG 
GATGCACTGG TGGCCCTCCT GGAGGAGCAG AGAGTTCGAG AAAAAGAGGA GGACAGTGAC 
CTGGAGGCTG TGATGCTGTC TAAGGGCTTC AGTCTGCATT GGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210768.1
CDS	227..5434
Translation	MWDGQSEMCQAHVLFDSFVQAATCKETLRAFQDLCEELNLNPGGQPQFYHTLRSRLHYWKAKALWAKLDKRACQKEYMRGHACTSTTCLIIGAGPCGLRTAIELGFLGARVVLVEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRKVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTAIRRQNYIHMYTGGVSSLALQIANQIQSQQAQAPKLLHRRESGSQKDLPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSSESDLEEEELVWKKGEELHARTNGERKLDLEEELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDEGEYCPWERERLSGVWLEEEEAGFVKESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNGSKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEAVEPFDGSPRRRPVYLCPCCSSEAKAKGTEHDHSVYVCTVFFLYVVQEPPLHQLRMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	XM_017355279.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355279.2

ORF Insert Sequence:

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGAGGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAG GGTGAGTACT GCCCTTGGGA GAGGGAACGA 
CTCTCAGGGG TGTGGCTTGA GGAAGAGGAG GCGGGGTTTG TTAAAGAATC TTATGAAGGA 
TGCAGCGACG ACCCTGATAT AGACGTCAGC TCACTCAGTG AGTCCAAGCT ATGTGACCAA 
CGGGAACAGG AGGAGTCTGT TCCTTTCCAC CAATCCCCTG CCTCTACAGA ATCTCCAGTC 
TCAATGAAAC CTTCAGAATC AGACCTCACA CCTGATCCGT CAACAACCCC CGAGAGCTCG 
CCTGCCAAGA GGTCAGAGGT GGTGGAGGAG TTCTGGATGA AGAGTGCAGA GATCCGAAAG 
AGTTTAGGCC TGACTCCGCT TTCTAGAGAA TTGCAGCCAA AACATATAGC AGCTTCAACC 
CAGACATCTA ATGTTAAAGA AAGCTTTTAC ACCTCAGTCA CATATAACAC CGCTGCTTTG 
GATTCTCCAA ACCAAAGTGC TAGAATCTGT GACTCCAGCA CACAAACTCA TTCTGTGACA 
GATCTTCAAG AAACCTCCCC TCTTGGACCT ACAGATGGCG ATGCTGGCGT CGATTTAGGA 
CGTTGTTCTG TTGTTCACAG ACTCAGCATC ACTGTAGAAG GTTGTGTGAT GGCAGATAAC 
CAGGGTTTGG ATTCTACCTC GTTTGCTACT GAAAGTGTTT TAAACCCAGA CGCAGGTCTT 
CCCACCCCTC CTTATAGTCC GTCTCACTCC CCTCTTGTTG GTAAACAGTG TCGTGCCCTT 
CACCATTCTG AGCCAATTCT AGACAGAGAG GTTATGGCTT TCTCATCTAC CTGCACTGCT 
TCTGTACCAC AGCGGAGCAG TTTTAAATCA GACCTCGAGC AAGCTCAAAG TCTTCCACCA 
GATGAAATCG AGATCCTGTG CGGAGACGAG GCGGAAAAAC TCCCGGAAAG ATCTTGCAGG 
ATGGAGAGCG GAGAGGTGGA TAACCGTAGA GCGGAGCACA GCAGGACGCT TCCTGACCGG 
GTGGTCGCAC CGCTGCTGGC AGGGGGACCA GAGGTCAGGC TTCGCAGGTC TGAGATGAAG 
TTGTGGGGTC CAGACGCAGA TGGTGATGTG AAGGAGAAAA AGCGCAGCTC GTTGTTTTCA 
CCCCGTAAAA GCAGGAAGAA TGGAAATGCA GCAGCTGAGA GCGGGCGAGA GACAGGGAAG 
CACAAGTCCC TCTGGAAGAC TGTTTTTTCA GTGTATAAGA AGGATAAAAA GAGGAAGGAG 
GTGGCAGTGG TGGCGGAAAC ACTTCCTGCT GCTAATAATG GGAGCAAACG GAAAGTGTCG 
GGAATTAACA GAACCACAGA CCTGTGTTTC CGGAAGAATC CAAGTTTCTC TGAAGATACG 
GATATGTCCT GTCATGCTCT TCTTGAAAGA TGTCCTCTCC GAGCTCAGAG AGCAGGAACA 
GAGGAGGAAC TCAATGCCAG GCTGACACGT CGAGTGCAGA GAGCAGCGCG CAGACAGGCC 
AAGCAGGAGG AGCTGCGGAG GCTGCATAGA GCTCAGATAA TCCAGCGTCA GTTGGAGCAG 
GTGGAAGTGA AGCAGAGGCA GCTGGAGGAG AAAGGTGTGG CTGTGGAGAA AGCACTCAGA 
GGAGAAGCAG TTGAGCCTTT TGACGGTTCT CCCCGTAGAA GGCCTGTATA TCTCTGTCCT 
TGCTGTTCCT CTGAAGCCAA AGCTAAAGGA ACCGAACATG ATCATTCGGT TTATGTCTGT 
ACAGTCTTTT TCCTGTACGT AGTGCAGGAG CCTCCACTTC ACCAACTGAG AATGGGAAAG 
AAAGACGATC CAAGCTTAAT GCATCAGTGG TTCAAACTGG TGCAGGAGAA GAATGCTCTC 
GTGCGTTATG AATCTGAGCT CATGATATTC GCTCGTGAAC TGGAGCTGGA GGACAGACAG 
AGTCGCCTGC AGCAGGAGCT GAGAGAGCGC ATGGCGGTGG ACGATCATCT AAAAGGAGAA 
GAGGAGCTGG CGGAGGAGAG GCGTATATTG AGCGAGATGC TGGATGTGGT GGAGCAGAGG 
GATGCACTGG TGGCCCTCCT GGAGGAGCAG AGAGTTCGAG AAAAAGAGGA GGACAGTGAC 
CTGGAGGCTG TGATGCTGTC TAAGGGCTTC AGTCTGCATT GGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( XM_017355279.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210768.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa58549
Clone ID Related Accession (Same CDS sequence)	XM_017355279.2 , XM_017355279.1 , XM_021475214.1
Accession Version	XM_017355279.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGAGGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAG GGTGAGTACT GCCCTTGGGA GAGGGAACGA 
CTCTCAGGGG TGTGGCTTGA GGAAGAGGAG GCGGGGTTTG TTAAAGAATC TTATGAAGGA 
TGCAGCGACG ACCCTGATAT AGACGTCAGC TCACTCAGTG AGTCCAAGCT ATGTGACCAA 
CGGGAACAGG AGGAGTCTGT TCCTTTCCAC CAATCCCCTG CCTCTACAGA ATCTCCAGTC 
TCAATGAAAC CTTCAGAATC AGACCTCACA CCTGATCCGT CAACAACCCC CGAGAGCTCG 
CCTGCCAAGA GGTCAGAGGT GGTGGAGGAG TTCTGGATGA AGAGTGCAGA GATCCGAAAG 
AGTTTAGGCC TGACTCCGCT TTCTAGAGAA TTGCAGCCAA AACATATAGC AGCTTCAACC 
CAGACATCTA ATGTTAAAGA AAGCTTTTAC ACCTCAGTCA CATATAACAC CGCTGCTTTG 
GATTCTCCAA ACCAAAGTGC TAGAATCTGT GACTCCAGCA CACAAACTCA TTCTGTGACA 
GATCTTCAAG AAACCTCCCC TCTTGGACCT ACAGATGGCG ATGCTGGCGT CGATTTAGGA 
CGTTGTTCTG TTGTTCACAG ACTCAGCATC ACTGTAGAAG GTTGTGTGAT GGCAGATAAC 
CAGGGTTTGG ATTCTACCTC GTTTGCTACT GAAAGTGTTT TAAACCCAGA CGCAGGTCTT 
CCCACCCCTC CTTATAGTCC GTCTCACTCC CCTCTTGTTG GTAAACAGTG TCGTGCCCTT 
CACCATTCTG AGCCAATTCT AGACAGAGAG GTTATGGCTT TCTCATCTAC CTGCACTGCT 
TCTGTACCAC AGCGGAGCAG TTTTAAATCA GACCTCGAGC AAGCTCAAAG TCTTCCACCA 
GATGAAATCG AGATCCTGTG CGGAGACGAG GCGGAAAAAC TCCCGGAAAG ATCTTGCAGG 
ATGGAGAGCG GAGAGGTGGA TAACCGTAGA GCGGAGCACA GCAGGACGCT TCCTGACCGG 
GTGGTCGCAC CGCTGCTGGC AGGGGGACCA GAGGTCAGGC TTCGCAGGTC TGAGATGAAG 
TTGTGGGGTC CAGACGCAGA TGGTGATGTG AAGGAGAAAA AGCGCAGCTC GTTGTTTTCA 
CCCCGTAAAA GCAGGAAGAA TGGAAATGCA GCAGCTGAGA GCGGGCGAGA GACAGGGAAG 
CACAAGTCCC TCTGGAAGAC TGTTTTTTCA GTGTATAAGA AGGATAAAAA GAGGAAGGAG 
GTGGCAGTGG TGGCGGAAAC ACTTCCTGCT GCTAATAATG GGAGCAAACG GAAAGTGTCG 
GGAATTAACA GAACCACAGA CCTGTGTTTC CGGAAGAATC CAAGTTTCTC TGAAGATACG 
GATATGTCCT GTCATGCTCT TCTTGAAAGA TGTCCTCTCC GAGCTCAGAG AGCAGGAACA 
GAGGAGGAAC TCAATGCCAG GCTGACACGT CGAGTGCAGA GAGCAGCGCG CAGACAGGCC 
AAGCAGGAGG AGCTGCGGAG GCTGCATAGA GCTCAGATAA TCCAGCGTCA GTTGGAGCAG 
GTGGAAGTGA AGCAGAGGCA GCTGGAGGAG AAAGGTGTGG CTGTGGAGAA AGCACTCAGA 
GGAGAAGCAG TTGAGCCTTT TGACGGTTCT CCCCGTAGAA GGCCTGTATA TCTCTGTCCT 
TGCTGTTCCT CTGAAGCCAA AGCTAAAGGA ACCGAACATG ATCATTCGGT TTATGTCTGT 
ACAGTCTTTT TCCTGTACGT AGTGCAGGAG CCTCCACTTC ACCAACTGAG AATGGGAAAG 
AAAGACGATC CAAGCTTAAT GCATCAGTGG TTCAAACTGG TGCAGGAGAA GAATGCTCTC 
GTGCGTTATG AATCTGAGCT CATGATATTC GCTCGTGAAC TGGAGCTGGA GGACAGACAG 
AGTCGCCTGC AGCAGGAGCT GAGAGAGCGC ATGGCGGTGG ACGATCATCT AAAAGGAGAA 
GAGGAGCTGG CGGAGGAGAG GCGTATATTG AGCGAGATGC TGGATGTGGT GGAGCAGAGG 
GATGCACTGG TGGCCCTCCT GGAGGAGCAG AGAGTTCGAG AAAAAGAGGA GGACAGTGAC 
CTGGAGGCTG TGATGCTGTC TAAGGGCTTC AGTCTGCATT GGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210768.1
CDS	296..5503
Translation	MWDGQSEMCQAHVLFDSFVQAATCKETLRAFQDLCEELNLNPGGQPQFYHTLRSRLHYWKAKALWAKLDKRACQKEYMRGHACTSTTCLIIGAGPCGLRTAIELGFLGARVVLVEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRKVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTAIRRQNYIHMYTGGVSSLALQIANQIQSQQAQAPKLLHRRESGSQKDLPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSSESDLEEEELVWKKGEELHARTNGERKLDLEEELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDEGEYCPWERERLSGVWLEEEEAGFVKESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNGSKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEAVEPFDGSPRRRPVYLCPCCSSEAKAKGTEHDHSVYVCTVFFLYVVQEPPLHQLRMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	XM_017355279.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355279.1

ORF Insert Sequence:

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGAGGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAG GGTGAGTACT GCCCTTGGGA GAGGGAACGA 
CTCTCAGGGG TGTGGCTTGA GGAAGAGGAG GCGGGGTTTG TTAAAGAATC TTATGAAGGA 
TGCAGCGACG ACCCTGATAT AGACGTCAGC TCACTCAGTG AGTCCAAGCT ATGTGACCAA 
CGGGAACAGG AGGAGTCTGT TCCTTTCCAC CAATCCCCTG CCTCTACAGA ATCTCCAGTC 
TCAATGAAAC CTTCAGAATC AGACCTCACA CCTGATCCGT CAACAACCCC CGAGAGCTCG 
CCTGCCAAGA GGTCAGAGGT GGTGGAGGAG TTCTGGATGA AGAGTGCAGA GATCCGAAAG 
AGTTTAGGCC TGACTCCGCT TTCTAGAGAA TTGCAGCCAA AACATATAGC AGCTTCAACC 
CAGACATCTA ATGTTAAAGA AAGCTTTTAC ACCTCAGTCA CATATAACAC CGCTGCTTTG 
GATTCTCCAA ACCAAAGTGC TAGAATCTGT GACTCCAGCA CACAAACTCA TTCTGTGACA 
GATCTTCAAG AAACCTCCCC TCTTGGACCT ACAGATGGCG ATGCTGGCGT CGATTTAGGA 
CGTTGTTCTG TTGTTCACAG ACTCAGCATC ACTGTAGAAG GTTGTGTGAT GGCAGATAAC 
CAGGGTTTGG ATTCTACCTC GTTTGCTACT GAAAGTGTTT TAAACCCAGA CGCAGGTCTT 
CCCACCCCTC CTTATAGTCC GTCTCACTCC CCTCTTGTTG GTAAACAGTG TCGTGCCCTT 
CACCATTCTG AGCCAATTCT AGACAGAGAG GTTATGGCTT TCTCATCTAC CTGCACTGCT 
TCTGTACCAC AGCGGAGCAG TTTTAAATCA GACCTCGAGC AAGCTCAAAG TCTTCCACCA 
GATGAAATCG AGATCCTGTG CGGAGACGAG GCGGAAAAAC TCCCGGAAAG ATCTTGCAGG 
ATGGAGAGCG GAGAGGTGGA TAACCGTAGA GCGGAGCACA GCAGGACGCT TCCTGACCGG 
GTGGTCGCAC CGCTGCTGGC AGGGGGACCA GAGGTCAGGC TTCGCAGGTC TGAGATGAAG 
TTGTGGGGTC CAGACGCAGA TGGTGATGTG AAGGAGAAAA AGCGCAGCTC GTTGTTTTCA 
CCCCGTAAAA GCAGGAAGAA TGGAAATGCA GCAGCTGAGA GCGGGCGAGA GACAGGGAAG 
CACAAGTCCC TCTGGAAGAC TGTTTTTTCA GTGTATAAGA AGGATAAAAA GAGGAAGGAG 
GTGGCAGTGG TGGCGGAAAC ACTTCCTGCT GCTAATAATG GGAGCAAACG GAAAGTGTCG 
GGAATTAACA GAACCACAGA CCTGTGTTTC CGGAAGAATC CAAGTTTCTC TGAAGATACG 
GATATGTCCT GTCATGCTCT TCTTGAAAGA TGTCCTCTCC GAGCTCAGAG AGCAGGAACA 
GAGGAGGAAC TCAATGCCAG GCTGACACGT CGAGTGCAGA GAGCAGCGCG CAGACAGGCC 
AAGCAGGAGG AGCTGCGGAG GCTGCATAGA GCTCAGATAA TCCAGCGTCA GTTGGAGCAG 
GTGGAAGTGA AGCAGAGGCA GCTGGAGGAG AAAGGTGTGG CTGTGGAGAA AGCACTCAGA 
GGAGAAGCAG TTGAGCCTTT TGACGGTTCT CCCCGTAGAA GGCCTGTATA TCTCTGTCCT 
TGCTGTTCCT CTGAAGCCAA AGCTAAAGGA ACCGAACATG ATCATTCGGT TTATGTCTGT 
ACAGTCTTTT TCCTGTACGT AGTGCAGGAG CCTCCACTTC ACCAACTGAG AATGGGAAAG 
AAAGACGATC CAAGCTTAAT GCATCAGTGG TTCAAACTGG TGCAGGAGAA GAATGCTCTC 
GTGCGTTATG AATCTGAGCT CATGATATTC GCTCGTGAAC TGGAGCTGGA GGACAGACAG 
AGTCGCCTGC AGCAGGAGCT GAGAGAGCGC ATGGCGGTGG ACGATCATCT AAAAGGAGAA 
GAGGAGCTGG CGGAGGAGAG GCGTATATTG AGCGAGATGC TGGATGTGGT GGAGCAGAGG 
GATGCACTGG TGGCCCTCCT GGAGGAGCAG AGAGTTCGAG AAAAAGAGGA GGACAGTGAC 
CTGGAGGCTG TGATGCTGTC TAAGGGCTTC AGTCTGCATT GGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( XM_021475214.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330889.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ODa58549
Clone ID Related Accession (Same CDS sequence)	XM_017355279.2 , XM_017355279.1 , XM_021475214.1
Accession Version	XM_021475214.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGAGGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAG GGTGAGTACT GCCCTTGGGA GAGGGAACGA 
CTCTCAGGGG TGTGGCTTGA GGAAGAGGAG GCGGGGTTTG TTAAAGAATC TTATGAAGGA 
TGCAGCGACG ACCCTGATAT AGACGTCAGC TCACTCAGTG AGTCCAAGCT ATGTGACCAA 
CGGGAACAGG AGGAGTCTGT TCCTTTCCAC CAATCCCCTG CCTCTACAGA ATCTCCAGTC 
TCAATGAAAC CTTCAGAATC AGACCTCACA CCTGATCCGT CAACAACCCC CGAGAGCTCG 
CCTGCCAAGA GGTCAGAGGT GGTGGAGGAG TTCTGGATGA AGAGTGCAGA GATCCGAAAG 
AGTTTAGGCC TGACTCCGCT TTCTAGAGAA TTGCAGCCAA AACATATAGC AGCTTCAACC 
CAGACATCTA ATGTTAAAGA AAGCTTTTAC ACCTCAGTCA CATATAACAC CGCTGCTTTG 
GATTCTCCAA ACCAAAGTGC TAGAATCTGT GACTCCAGCA CACAAACTCA TTCTGTGACA 
GATCTTCAAG AAACCTCCCC TCTTGGACCT ACAGATGGCG ATGCTGGCGT CGATTTAGGA 
CGTTGTTCTG TTGTTCACAG ACTCAGCATC ACTGTAGAAG GTTGTGTGAT GGCAGATAAC 
CAGGGTTTGG ATTCTACCTC GTTTGCTACT GAAAGTGTTT TAAACCCAGA CGCAGGTCTT 
CCCACCCCTC CTTATAGTCC GTCTCACTCC CCTCTTGTTG GTAAACAGTG TCGTGCCCTT 
CACCATTCTG AGCCAATTCT AGACAGAGAG GTTATGGCTT TCTCATCTAC CTGCACTGCT 
TCTGTACCAC AGCGGAGCAG TTTTAAATCA GACCTCGAGC AAGCTCAAAG TCTTCCACCA 
GATGAAATCG AGATCCTGTG CGGAGACGAG GCGGAAAAAC TCCCGGAAAG ATCTTGCAGG 
ATGGAGAGCG GAGAGGTGGA TAACCGTAGA GCGGAGCACA GCAGGACGCT TCCTGACCGG 
GTGGTCGCAC CGCTGCTGGC AGGGGGACCA GAGGTCAGGC TTCGCAGGTC TGAGATGAAG 
TTGTGGGGTC CAGACGCAGA TGGTGATGTG AAGGAGAAAA AGCGCAGCTC GTTGTTTTCA 
CCCCGTAAAA GCAGGAAGAA TGGAAATGCA GCAGCTGAGA GCGGGCGAGA GACAGGGAAG 
CACAAGTCCC TCTGGAAGAC TGTTTTTTCA GTGTATAAGA AGGATAAAAA GAGGAAGGAG 
GTGGCAGTGG TGGCGGAAAC ACTTCCTGCT GCTAATAATG GGAGCAAACG GAAAGTGTCG 
GGAATTAACA GAACCACAGA CCTGTGTTTC CGGAAGAATC CAAGTTTCTC TGAAGATACG 
GATATGTCCT GTCATGCTCT TCTTGAAAGA TGTCCTCTCC GAGCTCAGAG AGCAGGAACA 
GAGGAGGAAC TCAATGCCAG GCTGACACGT CGAGTGCAGA GAGCAGCGCG CAGACAGGCC 
AAGCAGGAGG AGCTGCGGAG GCTGCATAGA GCTCAGATAA TCCAGCGTCA GTTGGAGCAG 
GTGGAAGTGA AGCAGAGGCA GCTGGAGGAG AAAGGTGTGG CTGTGGAGAA AGCACTCAGA 
GGAGAAGCAG TTGAGCCTTT TGACGGTTCT CCCCGTAGAA GGCCTGTATA TCTCTGTCCT 
TGCTGTTCCT CTGAAGCCAA AGCTAAAGGA ACCGAACATG ATCATTCGGT TTATGTCTGT 
ACAGTCTTTT TCCTGTACGT AGTGCAGGAG CCTCCACTTC ACCAACTGAG AATGGGAAAG 
AAAGACGATC CAAGCTTAAT GCATCAGTGG TTCAAACTGG TGCAGGAGAA GAATGCTCTC 
GTGCGTTATG AATCTGAGCT CATGATATTC GCTCGTGAAC TGGAGCTGGA GGACAGACAG 
AGTCGCCTGC AGCAGGAGCT GAGAGAGCGC ATGGCGGTGG ACGATCATCT AAAAGGAGAA 
GAGGAGCTGG CGGAGGAGAG GCGTATATTG AGCGAGATGC TGGATGTGGT GGAGCAGAGG 
GATGCACTGG TGGCCCTCCT GGAGGAGCAG AGAGTTCGAG AAAAAGAGGA GGACAGTGAC 
CTGGAGGCTG TGATGCTGTC TAAGGGCTTC AGTCTGCATT GGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330889.1
CDS	231..5438
Translation	MWDGQSEMCQAHVLFDSFVQAATCKETLRAFQDLCEELNLNPGGQPQFYHTLRSRLHYWKAKALWAKLDKRACQKEYMRGHACTSTTCLIIGAGPCGLRTAIELGFLGARVVLVEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRKVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTAIRRQNYIHMYTGGVSSLALQIANQIQSQQAQAPKLLHRRESGSQKDLPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSSESDLEEEELVWKKGEELHARTNGERKLDLEEELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDEGEYCPWERERLSGVWLEEEEAGFVKESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNGSKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEAVEPFDGSPRRRPVYLCPCCSSEAKAKGTEHDHSVYVCTVFFLYVVQEPPLHQLRMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	XM_021475214.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475214.1

ORF Insert Sequence:

ATGTGGGACG GTCAGTCTGA GATGTGTCAG GCGCACGTCC TGTTCGACTC CTTCGTTCAG 
GCCGCCACAT GCAAGGAGAC CCTCCGAGCC TTTCAGGATC TGTGTGAAGA GTTAAATCTG 
AATCCCGGCG GTCAACCGCA GTTCTACCAC ACCCTGAGGA GCCGACTGCA CTACTGGAAG 
GCCAAAGCAC TGTGGGCCAA ACTGGACAAA AGAGCCTGCC AGAAAGAATA CATGAGAGGC 
CACGCGTGCA CCAGCACCAC GTGTCTGATC ATAGGTGCAG GTCCGTGTGG GCTGAGGACG 
GCTATAGAGC TGGGATTTCT GGGCGCCAGG GTAGTTCTGG TGGAAAAGCG GGATGCTTTC 
TCTAGGAACA ATGTCCTGCA TCTCTGGCCC TTCACCATCC AGGACCTACG GGGTCTGGGG 
GCCAAAAAGT TTTACGGGAA ATTCTGTGCT GGTGCTATAG ACCATATCAG TATTCGTCAG 
TTGCAGTTGA TGCTTTTAAA GGTGGCCTTG TTATTGGGTG TCGAAGTTCA TGTGAACGTG 
GAGTTCAAAC ATCTACTGGA ACCTCCAGAG AACCAGGAGA AACGTGTTGG TTGGCGGGCA 
GAGGTTCAGC CCAGCTCTCA TCCAGTCAGA CAGCTGGAGT TTGATGTGGT TATTGGGGCA 
GACGGCAGAA GAAACACATT ACCAGGGTTT CGCAGGAAGG AGTTTCGTGG GAAACTGGCC 
ATTGCCATCA CGGCGAATTT CATCAATCGC AACACCACCG CCGAGGCTAA AGTGGAGGAG 
ATCAGCGGCG TCGCCTTCAT TTTCAACCAG AGGTTTTTCC AGGACCTCCG GCAAGCTACC 
GGAATCGATC TGGAAAATAT AGTATACTAT AAAGACGACA CTCACTATTT CGTCATGACC 
GCCAAAAAAC AAAGTCTTCT GGAGAAAGGA GTCATCCTGC GTGATTATGC TGACACTGAG 
ATGCTGCTGT CCAGACATAA TGTGGACCAG AATGCACTGC TCTCGTATGC ACGTGAAGCG 
GCTGATTTCT CCACCAATCA TCAGCTTCCA GCGCTGGATT TTGCCATCAA TCACTATGGG 
CAGTCAGATG TGGCCATGTT TGACTTTACC TGTATGTACG CCTCGGAAAA CGCAGCGATG 
GTACGTCAGC GTATGGGACA TCCACTGCTC GTTGCTCTGG TGGGCGACAG TTTGCTGGAG 
CCGTTCTGGC CAATGGGAAC AGGGATAGCT CGGGGATTCC TGGCAGCGAT GGACACAGCG 
TGGATGGTGC GCAGCTGGGG TCAGGGCAAC ACTCCTCTGG AGGTTCTGGC TGAGAGAGAG 
AGTGTGTATA GGTTGCTTCC TCAGACAACT CCAGAAAATG TCAGTAAAAA CTACAGCCAG 
TTCAGTGTGG ATCCTGCTAG TCGCTATCCC AACATTAATA TGCAGCTCAT CAACGCCGCT 
CAGGTGCGTC ACCTCATAGA CACAGGTGAG GGTCCAGTTT TGGGTCTCGA TGCTGTTAGT 
TCTCCACACC CAAGACTTAC GCGACAAGAG TCAATGGCTC GGTACAGTAA ACTGCTGAGC 
TGGTGTCAGG AACACACTCA TGGTTACAGG AAAGTGTGTG TGACGGACTT CACCTCTTCC 
TGGAGGAGCG GACTGGCCCT GTGTGCTCTC ATTCACACCT TCAGGCCTGA CCTCATTGAT 
TTTGCATCTC TGGAGGAGAG CGAGGCAGAG TTCAGTGGTC AGCTGGGTTT GGACGTGGCG 
GAGCAGGAAT TTGGTATTTG TCCTATAATG ACCGGCAAGG AGATGAGTGT GCTGGAAGAG 
AGCGACTCTC TCTGTATGGT CATGTACCTC AGTCAGCTTC ATGAGCTCCT CAAAGACACA 
TCGCCACCTA GTGGAAGTCA GAGCTCAGAA GAGAGAGCGG TTTTGTTCTC CAGCAGCAGA 
TCACCAATCT CACTGCTCAG CAAACTGGGA CAGAGTTTGT CCCGCAAACG CAATCCTAAG 
GATAAGAAAG AGAAGGAAGC AGACAGTGTT GGAAAGAGGA GGAGAACAAG TCAGGCCGGC 
CAATCGGAAG AGGAGGATGC TCTTCATGAC GGCAATGAAA ATAAGTCTCC AGCAGAAACT 
CCCGGCTCAG AACCGAAGGC CTCTGAGGGT CACAGCAAGG TTCGGTCCAT GGCGTCGCTC 
CTTCTGGCTA AATTTGAGGA GAACTCGCCA TCGCCTTCAA CCACTGCAAT ACGCAGACAG 
AACTACATTC ACATGTATAC CGGCGGAGTG AGCTCATTGG CTCTGCAGAT AGCCAATCAG 
ATTCAAAGTC AGCAGGCTCA GGCTCCCAAG CTCCTTCACA GGAGGGAGTC GGGTTCTCAG 
AAGGATCTTC CAGTAAATGT CGGCAGTAGT GATGTGTGTT ATTTCTGTGG CCGTCGCGTT 
TATGTCATGG AGAGGCTGAG CGCAGAGGGA AAGTTCTTCC ACAGGAGCTG CTTCCAGTGC 
GACCACTGCA GCTCCACGAT ACGCCTCTCC AACTACACTT ATGACCAGCT ACATGGCAAG 
TTTTACTGTA AGCATCACTT CAGCTTCAGA CTGGCAAGTG TGGCTCAGAG AAAGAGACCG 
GCCCCACCGG TGGCCCCTCG GCCTGCTCAG GCTTCCCTGG CTGCATCCTC GGCCTCTACC 
TCTTTGTCCT CTTTGGGCTC AGTGGGCACA GCCACACCAG ACAGCTGGTC CTCCAGCACC 
CACACAGACA TGGCATCCAG CTTGGCCAAG AGACTGTGCG GCACACCAGA ACGCATTGAG 
CTTGAGAATT ACAAACCCTC CCCACAGAAA CAAGACAGCC CCCTGCAGGA GGTACCAGAG 
GAAACCCTCG CACAGCACAA TCTCAGCGCT AGCCTGCAGG AGAAGAATGC TGAAGAGCAG 
TCCAGTAGCT CTGAGTCAGA TCTAGAGGAG GAGGAGCTTG TCTGGAAGAA GGGGGAGGAG 
CTACATGCCA GGACCAATGG AGAGAGGAAG CTTGACCTAG AGGAGGAGCT GAAGGAAGAA 
GAGGGAGGGG AGAAGCTGGA AAAACAAGAG GGGGAGGAAG AAGGAGAAGT TTCAGAAGAA 
GAGCAAGACG AGGGAGATTC TAGTGATGAG GGTGAGTACT GCCCTTGGGA GAGGGAACGA 
CTCTCAGGGG TGTGGCTTGA GGAAGAGGAG GCGGGGTTTG TTAAAGAATC TTATGAAGGA 
TGCAGCGACG ACCCTGATAT AGACGTCAGC TCACTCAGTG AGTCCAAGCT ATGTGACCAA 
CGGGAACAGG AGGAGTCTGT TCCTTTCCAC CAATCCCCTG CCTCTACAGA ATCTCCAGTC 
TCAATGAAAC CTTCAGAATC AGACCTCACA CCTGATCCGT CAACAACCCC CGAGAGCTCG 
CCTGCCAAGA GGTCAGAGGT GGTGGAGGAG TTCTGGATGA AGAGTGCAGA GATCCGAAAG 
AGTTTAGGCC TGACTCCGCT TTCTAGAGAA TTGCAGCCAA AACATATAGC AGCTTCAACC 
CAGACATCTA ATGTTAAAGA AAGCTTTTAC ACCTCAGTCA CATATAACAC CGCTGCTTTG 
GATTCTCCAA ACCAAAGTGC TAGAATCTGT GACTCCAGCA CACAAACTCA TTCTGTGACA 
GATCTTCAAG AAACCTCCCC TCTTGGACCT ACAGATGGCG ATGCTGGCGT CGATTTAGGA 
CGTTGTTCTG TTGTTCACAG ACTCAGCATC ACTGTAGAAG GTTGTGTGAT GGCAGATAAC 
CAGGGTTTGG ATTCTACCTC GTTTGCTACT GAAAGTGTTT TAAACCCAGA CGCAGGTCTT 
CCCACCCCTC CTTATAGTCC GTCTCACTCC CCTCTTGTTG GTAAACAGTG TCGTGCCCTT 
CACCATTCTG AGCCAATTCT AGACAGAGAG GTTATGGCTT TCTCATCTAC CTGCACTGCT 
TCTGTACCAC AGCGGAGCAG TTTTAAATCA GACCTCGAGC AAGCTCAAAG TCTTCCACCA 
GATGAAATCG AGATCCTGTG CGGAGACGAG GCGGAAAAAC TCCCGGAAAG ATCTTGCAGG 
ATGGAGAGCG GAGAGGTGGA TAACCGTAGA GCGGAGCACA GCAGGACGCT TCCTGACCGG 
GTGGTCGCAC CGCTGCTGGC AGGGGGACCA GAGGTCAGGC TTCGCAGGTC TGAGATGAAG 
TTGTGGGGTC CAGACGCAGA TGGTGATGTG AAGGAGAAAA AGCGCAGCTC GTTGTTTTCA 
CCCCGTAAAA GCAGGAAGAA TGGAAATGCA GCAGCTGAGA GCGGGCGAGA GACAGGGAAG 
CACAAGTCCC TCTGGAAGAC TGTTTTTTCA GTGTATAAGA AGGATAAAAA GAGGAAGGAG 
GTGGCAGTGG TGGCGGAAAC ACTTCCTGCT GCTAATAATG GGAGCAAACG GAAAGTGTCG 
GGAATTAACA GAACCACAGA CCTGTGTTTC CGGAAGAATC CAAGTTTCTC TGAAGATACG 
GATATGTCCT GTCATGCTCT TCTTGAAAGA TGTCCTCTCC GAGCTCAGAG AGCAGGAACA 
GAGGAGGAAC TCAATGCCAG GCTGACACGT CGAGTGCAGA GAGCAGCGCG CAGACAGGCC 
AAGCAGGAGG AGCTGCGGAG GCTGCATAGA GCTCAGATAA TCCAGCGTCA GTTGGAGCAG 
GTGGAAGTGA AGCAGAGGCA GCTGGAGGAG AAAGGTGTGG CTGTGGAGAA AGCACTCAGA 
GGAGAAGCAG TTGAGCCTTT TGACGGTTCT CCCCGTAGAA GGCCTGTATA TCTCTGTCCT 
TGCTGTTCCT CTGAAGCCAA AGCTAAAGGA ACCGAACATG ATCATTCGGT TTATGTCTGT 
ACAGTCTTTT TCCTGTACGT AGTGCAGGAG CCTCCACTTC ACCAACTGAG AATGGGAAAG 
AAAGACGATC CAAGCTTAAT GCATCAGTGG TTCAAACTGG TGCAGGAGAA GAATGCTCTC 
GTGCGTTATG AATCTGAGCT CATGATATTC GCTCGTGAAC TGGAGCTGGA GGACAGACAG 
AGTCGCCTGC AGCAGGAGCT GAGAGAGCGC ATGGCGGTGG ACGATCATCT AAAAGGAGAA 
GAGGAGCTGG CGGAGGAGAG GCGTATATTG AGCGAGATGC TGGATGTGGT GGAGCAGAGG 
GATGCACTGG TGGCCCTCCT GGAGGAGCAG AGAGTTCGAG AAAAAGAGGA GGACAGTGAC 
CTGGAGGCTG TGATGCTGTC TAAGGGCTTC AGTCTGCATT GGGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( XM_017355280.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210769.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X8, mRNA.

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CloneID	ODa58550
Clone ID Related Accession (Same CDS sequence)	XM_017355280.1
Accession Version	XM_017355280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAATT GTCTGATCAT AGGTGCAGGT CCGTGTGGGC TGAGGACGGC TATAGAGCTG 
GGATTTCTGG GCGCCAGGGT AGTTCTGGTG GAAAAGCGGG ATGCTTTCTC TAGGAACAAT 
GTCCTGCATC TCTGGCCCTT CACCATCCAG GACCTACGGG GTCTGGGGGC CAAAAAGTTT 
TACGGGAAAT TCTGTGCTGG TGCTATAGAC CATATCAGTA TTCGTCAGTT GCAGTTGATG 
CTTTTAAAGG TGGCCTTGTT ATTGGGTGTC GAAGTTCATG TGAACGTGGA GTTCAAACAT 
CTACTGGAAC CTCCAGAGAA CCAGGAGAAA CGTGTTGGTT GGCGGGCAGA GGTTCAGCCC 
AGCTCTCATC CAGTCAGACA GCTGGAGTTT GATGTGGTTA TTGGGGCAGA CGGCAGAAGA 
AACACATTAC CAGGGTTTCG CAGGAAGGAG TTTCGTGGGA AACTGGCCAT TGCCATCACG 
GCGAATTTCA TCAATCGCAA CACCACCGCC GAGGCTAAAG TGGAGGAGAT CAGCGGCGTC 
GCCTTCATTT TCAACCAGAG GTTTTTCCAG GACCTCCGGC AAGCTACCGG AATCGATCTG 
GAAAATATAG TATACTATAA AGACGACACT CACTATTTCG TCATGACCGC CAAAAAACAA 
AGTCTTCTGG AGAAAGGAGT CATCCTGCGT GATTATGCTG ACACTGAGAT GCTGCTGTCC 
AGACATAATG TGGACCAGAA TGCACTGCTC TCGTATGCAC GTGAAGCGGC TGATTTCTCC 
ACCAATCATC AGCTTCCAGC GCTGGATTTT GCCATCAATC ACTATGGGCA GTCAGATGTG 
GCCATGTTTG ACTTTACCTG TATGTACGCC TCGGAAAACG CAGCGATGGT ACGTCAGCGT 
ATGGGACATC CACTGCTCGT TGCTCTGGTG GGCGACAGTT TGCTGGAGCC GTTCTGGCCA 
ATGGGAACAG GGATAGCTCG GGGATTCCTG GCAGCGATGG ACACAGCGTG GATGGTGCGC 
AGCTGGGGTC AGGGCAACAC TCCTCTGGAG GTTCTGGCTG AGAGAGAGAG TGTGTATAGG 
TTGCTTCCTC AGACAACTCC AGAAAATGTC AGTAAAAACT ACAGCCAGTT CAGTGTGGAT 
CCTGCTAGTC GCTATCCCAA CATTAATATG CAGCTCATCA ACGCCGCTCA GGTGCGTCAC 
CTCATAGACA CAGGTGAGGG TCCAGTTTTG GGTCTCGATG CTGTTAGTTC TCCACACCCA 
AGACTTACGC GACAAGAGTC AATGGCTCGG TACAGTAAAC TGCTGAGCTG GTGTCAGGAA 
CACACTCATG GTTACAGGAA AGTGTGTGTG ACGGACTTCA CCTCTTCCTG GAGGAGCGGA 
CTGGCCCTGT GTGCTCTCAT TCACACCTTC AGGCCTGACC TCATTGATTT TGCATCTCTG 
GAGGAGAGCG AGGCAGAGTT CAGTGGTCAG CTGGGTTTGG ACGTGGCGGA GCAGGAATTT 
GGTATTTGTC CTATAATGAC CGGCAAGGAG ATGAGTGTGC TGGAAGAGAG CGACTCTCTC 
TGTATGGTCA TGTACCTCAG TCAGCTTCAT GAGCTCCTCA AAGACACATC GCCACCTAGT 
GGAAGTCAGA GCTCAGAAGA GAGAGCGGTT TTGTTCTCCA GCAGCAGATC ACCAATCTCA 
CTGCTCAGCA AACTGGGACA GAGTTTGTCC CGCAAACGCA ATCCTAAGGA TAAGAAAGAG 
AAGGAAGCAG ACAGTGTTGG AAAGAGGAGG AGAACAAGTC AGGCCGGCCA ATCGGAAGAG 
GAGGATGCTC TTCATGACGG CAATGAAAAT AAGTCTCCAG CAGAAACTCC CGGCTCAGAA 
CCGAAGGCCT CTGAGGGTCA CAGCAAGGTT CGGTCCATGG CGTCGCTCCT TCTGGCTAAA 
TTTGAGGAGA ACTCGCCATC GCCTTCAACC ACTGCAATAC GCAGACAGAA CTACATTCAC 
ATGTATACCG GCGGAGTGAG CTCATTGGCT CTGCAGATAG CCAATCAGAT TCAAAGTCAG 
CAGGCTCAGG CTCCCAAGCT CCTTCACAGG AGGGAGTCGG GTTCTCAGAA GGATCTTCCA 
GTAAATGTCG GCAGTAGTGA TGTGTGTTAT TTCTGTGGCC GTCGCGTTTA TGTCATGGAG 
AGGCTGAGCG CAGAGGGAAA GTTCTTCCAC AGGAGCTGCT TCCAGTGCGA CCACTGCAGC 
TCCACGATAC GCCTCTCCAA CTACACTTAT GACCAGCTAC ATGGCAAGTT TTACTGTAAG 
CATCACTTCA GCTTCAGACT GGCAAGTGTG GCTCAGAGAA AGAGACCGGC CCCACCGGTG 
GCCCCTCGGC CTGCTCAGGC TTCCCTGGCT GCATCCTCGG CCTCTACCTC TTTGTCCTCT 
TTGGGCTCAG TGGGCACAGC CACACCAGAC AGCTGGTCCT CCAGCACCCA CACAGACATG 
GCATCCAGCT TGGCCAAGAG ACTGTGCGGC ACACCAGAAC GCATTGAGCT TGAGAATTAC 
AAACCCTCCC CACAGAAACA AGACAGCCCC CTGCAGGAGG TACCAGAGGA AACCCTCGCA 
CAGCACAATC TCAGCGCTAG CCTGCAGGAG AAGAATGCTG AAGAGCAGTC CAGTAGCTCT 
GAGTCAGATC TAGAGGAGGA GGAGCTTGTC TGGAAGAAGG GGGAGGAGCT ACATGCCAGG 
ACCAATGGAG AGAGGAAGCT TGACCTAGAG GAGGAGCTGA AGGAAGAAGA GGGAGGGGAG 
AAGCTGGAAA AACAAGAGGG GGAGGAAGAA GGAGAAGTTT CAGAAGAAGA GCAAGACGAG 
GGAGATTCTA GTGATGAGGG TGAGTACTGC CCTTGGGAGA GGGAACGACT CTCAGGGGTG 
TGGCTTGAGG AAGAGGAGGC GGGGTTTGTT AAAGAATCTT ATGAAGGATG CAGCGACGAC 
CCTGATATAG ACGTCAGCTC ACTCAGTGAG TCCAAGCTAT GTGACCAACG GGAACAGGAG 
GAGTCTGTTC CTTTCCACCA ATCCCCTGCC TCTACAGAAT CTCCAGTCTC AATGAAACCT 
TCAGAATCAG ACCTCACACC TGATCCGTCA ACAACCCCCG AGAGCTCGCC TGCCAAGAGG 
TCAGAGGTGG TGGAGGAGTT CTGGATGAAG AGTGCAGAGA TCCGAAAGAG TTTAGGCCTG 
ACTCCGCTTT CTAGAGAATT GCAGCCAAAA CATATAGCAG CTTCAACCCA GACATCTAAT 
GTTAAAGAAA GCTTTTACAC CTCAGTCACA TATAACACCG CTGCTTTGGA TTCTCCAAAC 
CAAAGTGCTA GAATCTGTGA CTCCAGCACA CAAACTCATT CTGTGACAGA TCTTCAAGAA 
ACCTCCCCTC TTGGACCTAC AGATGGCGAT GCTGGCGTCG ATTTAGGACG TTGTTCTGTT 
GTTCACAGAC TCAGCATCAC TGTAGAAGGT TGTGTGATGG CAGATAACCA GGGTTTGGAT 
TCTACCTCGT TTGCTACTGA AAGTGTTTTA AACCCAGACG CAGGTCTTCC CACCCCTCCT 
TATAGTCCGT CTCACTCCCC TCTTGTTGGT AAACAGTGTC GTGCCCTTCA CCATTCTGAG 
CCAATTCTAG ACAGAGAGGT TATGGCTTTC TCATCTACCT GCACTGCTTC TGTACCACAG 
CGGAGCAGTT TTAAATCAGA CCTCGAGCAA GCTCAAAGTC TTCCACCAGA TGAAATCGAG 
ATCCTGTGCG GAGACGAGGC GGAAAAACTC CCGGAAAGAT CTTGCAGGAT GGAGAGCGGA 
GAGGTGGATA ACCGTAGAGC GGAGCACAGC AGGACGCTTC CTGACCGGGT GGTCGCACCG 
CTGCTGGCAG GGGGACCAGA GGTCAGGCTT CGCAGGTCTG AGATGAAGTT GTGGGGTCCA 
GACGCAGATG GTGATGTGAA GGAGAAAAAG CGCAGCTCGT TGTTTTCACC CCGTAAAAGC 
AGGAAGAATG GAAATGCAGC AGCTGAGAGC GGGCGAGAGA CAGGGAAGCA CAAGTCCCTC 
TGGAAGACTG TTTTTTCAGT GTATAAGAAG GATAAAAAGA GGAAGGAGGT GGCAGTGGTG 
GCGGAAACAC TTCCTGCTGC TAATAATGGG AGCAAACGGA AAGTGTCGGG AATTAACAGA 
ACCACAGACC TGTGTTTCCG GAAGAATCCA AGTTTCTCTG AAGATACGGA TATGTCCTGT 
CATGCTCTTC TTGAAAGATG TCCTCTCCGA GCTCAGAGAG CAGGAACAGA GGAGGAACTC 
AATGCCAGGC TGACACGTCG AGTGCAGAGA GCAGCGCGCA GACAGGCCAA GCAGGAGGAG 
CTGCGGAGGC TGCATAGAGC TCAGATAATC CAGCGTCAGT TGGAGCAGGT GGAAGTGAAG 
CAGAGGCAGC TGGAGGAGAA AGGTGTGGCT GTGGAGAAAG CACTCAGAGG AGAAGCAGTT 
GAGCCTTTTG ACGGTTCTCC CCGTAGAAGG CCTGTATATC TCTGTCCTTG CTGTTCCTCT 
GAAGGTAAAA CCGGCCCTGC TTCCTTTCTC ACTCCTCTAG TGCAGATTTT AGCAATACTT 
TACAATCCTT TTTACCTTTT CTTCCTCTCT GCTCATTCGT CCTGCCTCCT GATGTGTGCA 
GCCAAAGCTA AAGGAACCGA ACATGATCAT TCGGTTTATG TCTGTACAGT CTTTTTCCTG 
TACGTAGTGC AGGAGCCTCC ACTTCACCAA CTGAGAATGG GAAAGAAAGA CGATCCAAGC 
TTAATGCATC AGTGGTTCAA ACTGGTGCAG GAGAAGAATG CTCTCGTGCG TTATGAATCT 
GAGCTCATGA TATTCGCTCG TGAACTGGAG CTGGAGGACA GACAGAGTCG CCTGCAGCAG 
GAGCTGAGAG AGCGCATGGC GGTGGACGAT CATCTAAAAG GAGAAGAGGA GCTGGCGGAG 
GAGAGGCGTA TATTGAGCGA GATGCTGGAT GTGGTGGAGC AGAGGGATGC ACTGGTGGCC 
CTCCTGGAGG AGCAGAGAGT TCGAGAAAAA GAGGAGGACA GTGACCTGGA GGCTGTGATG 
CTGTCTAAGG GCTTCAGTCT GCATTGGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210769.1
CDS	24..5096
Translation	MQNCLIIGAGPCGLRTAIELGFLGARVVLVEKRDAFSRNNVLHLWPFTIQDLRGLGAKKFYGKFCAGAIDHISIRQLQLMLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRKVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTAIRRQNYIHMYTGGVSSLALQIANQIQSQQAQAPKLLHRRESGSQKDLPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSSESDLEEEELVWKKGEELHARTNGERKLDLEEELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDEGEYCPWERERLSGVWLEEEEAGFVKESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNGSKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEAVEPFDGSPRRRPVYLCPCCSSEGKTGPASFLTPLVQILAILYNPFYLFFLSAHSSCLLMCAAKAKGTEHDHSVYVCTVFFLYVVQEPPLHQLRMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	XM_017355280.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355280.1

ORF Insert Sequence:

ATGCAAAATT GTCTGATCAT AGGTGCAGGT CCGTGTGGGC TGAGGACGGC TATAGAGCTG 
GGATTTCTGG GCGCCAGGGT AGTTCTGGTG GAAAAGCGGG ATGCTTTCTC TAGGAACAAT 
GTCCTGCATC TCTGGCCCTT CACCATCCAG GACCTACGGG GTCTGGGGGC CAAAAAGTTT 
TACGGGAAAT TCTGTGCTGG TGCTATAGAC CATATCAGTA TTCGTCAGTT GCAGTTGATG 
CTTTTAAAGG TGGCCTTGTT ATTGGGTGTC GAAGTTCATG TGAACGTGGA GTTCAAACAT 
CTACTGGAAC CTCCAGAGAA CCAGGAGAAA CGTGTTGGTT GGCGGGCAGA GGTTCAGCCC 
AGCTCTCATC CAGTCAGACA GCTGGAGTTT GATGTGGTTA TTGGGGCAGA CGGCAGAAGA 
AACACATTAC CAGGGTTTCG CAGGAAGGAG TTTCGTGGGA AACTGGCCAT TGCCATCACG 
GCGAATTTCA TCAATCGCAA CACCACCGCC GAGGCTAAAG TGGAGGAGAT CAGCGGCGTC 
GCCTTCATTT TCAACCAGAG GTTTTTCCAG GACCTCCGGC AAGCTACCGG AATCGATCTG 
GAAAATATAG TATACTATAA AGACGACACT CACTATTTCG TCATGACCGC CAAAAAACAA 
AGTCTTCTGG AGAAAGGAGT CATCCTGCGT GATTATGCTG ACACTGAGAT GCTGCTGTCC 
AGACATAATG TGGACCAGAA TGCACTGCTC TCGTATGCAC GTGAAGCGGC TGATTTCTCC 
ACCAATCATC AGCTTCCAGC GCTGGATTTT GCCATCAATC ACTATGGGCA GTCAGATGTG 
GCCATGTTTG ACTTTACCTG TATGTACGCC TCGGAAAACG CAGCGATGGT ACGTCAGCGT 
ATGGGACATC CACTGCTCGT TGCTCTGGTG GGCGACAGTT TGCTGGAGCC GTTCTGGCCA 
ATGGGAACAG GGATAGCTCG GGGATTCCTG GCAGCGATGG ACACAGCGTG GATGGTGCGC 
AGCTGGGGTC AGGGCAACAC TCCTCTGGAG GTTCTGGCTG AGAGAGAGAG TGTGTATAGG 
TTGCTTCCTC AGACAACTCC AGAAAATGTC AGTAAAAACT ACAGCCAGTT CAGTGTGGAT 
CCTGCTAGTC GCTATCCCAA CATTAATATG CAGCTCATCA ACGCCGCTCA GGTGCGTCAC 
CTCATAGACA CAGGTGAGGG TCCAGTTTTG GGTCTCGATG CTGTTAGTTC TCCACACCCA 
AGACTTACGC GACAAGAGTC AATGGCTCGG TACAGTAAAC TGCTGAGCTG GTGTCAGGAA 
CACACTCATG GTTACAGGAA AGTGTGTGTG ACGGACTTCA CCTCTTCCTG GAGGAGCGGA 
CTGGCCCTGT GTGCTCTCAT TCACACCTTC AGGCCTGACC TCATTGATTT TGCATCTCTG 
GAGGAGAGCG AGGCAGAGTT CAGTGGTCAG CTGGGTTTGG ACGTGGCGGA GCAGGAATTT 
GGTATTTGTC CTATAATGAC CGGCAAGGAG ATGAGTGTGC TGGAAGAGAG CGACTCTCTC 
TGTATGGTCA TGTACCTCAG TCAGCTTCAT GAGCTCCTCA AAGACACATC GCCACCTAGT 
GGAAGTCAGA GCTCAGAAGA GAGAGCGGTT TTGTTCTCCA GCAGCAGATC ACCAATCTCA 
CTGCTCAGCA AACTGGGACA GAGTTTGTCC CGCAAACGCA ATCCTAAGGA TAAGAAAGAG 
AAGGAAGCAG ACAGTGTTGG AAAGAGGAGG AGAACAAGTC AGGCCGGCCA ATCGGAAGAG 
GAGGATGCTC TTCATGACGG CAATGAAAAT AAGTCTCCAG CAGAAACTCC CGGCTCAGAA 
CCGAAGGCCT CTGAGGGTCA CAGCAAGGTT CGGTCCATGG CGTCGCTCCT TCTGGCTAAA 
TTTGAGGAGA ACTCGCCATC GCCTTCAACC ACTGCAATAC GCAGACAGAA CTACATTCAC 
ATGTATACCG GCGGAGTGAG CTCATTGGCT CTGCAGATAG CCAATCAGAT TCAAAGTCAG 
CAGGCTCAGG CTCCCAAGCT CCTTCACAGG AGGGAGTCGG GTTCTCAGAA GGATCTTCCA 
GTAAATGTCG GCAGTAGTGA TGTGTGTTAT TTCTGTGGCC GTCGCGTTTA TGTCATGGAG 
AGGCTGAGCG CAGAGGGAAA GTTCTTCCAC AGGAGCTGCT TCCAGTGCGA CCACTGCAGC 
TCCACGATAC GCCTCTCCAA CTACACTTAT GACCAGCTAC ATGGCAAGTT TTACTGTAAG 
CATCACTTCA GCTTCAGACT GGCAAGTGTG GCTCAGAGAA AGAGACCGGC CCCACCGGTG 
GCCCCTCGGC CTGCTCAGGC TTCCCTGGCT GCATCCTCGG CCTCTACCTC TTTGTCCTCT 
TTGGGCTCAG TGGGCACAGC CACACCAGAC AGCTGGTCCT CCAGCACCCA CACAGACATG 
GCATCCAGCT TGGCCAAGAG ACTGTGCGGC ACACCAGAAC GCATTGAGCT TGAGAATTAC 
AAACCCTCCC CACAGAAACA AGACAGCCCC CTGCAGGAGG TACCAGAGGA AACCCTCGCA 
CAGCACAATC TCAGCGCTAG CCTGCAGGAG AAGAATGCTG AAGAGCAGTC CAGTAGCTCT 
GAGTCAGATC TAGAGGAGGA GGAGCTTGTC TGGAAGAAGG GGGAGGAGCT ACATGCCAGG 
ACCAATGGAG AGAGGAAGCT TGACCTAGAG GAGGAGCTGA AGGAAGAAGA GGGAGGGGAG 
AAGCTGGAAA AACAAGAGGG GGAGGAAGAA GGAGAAGTTT CAGAAGAAGA GCAAGACGAG 
GGAGATTCTA GTGATGAGGG TGAGTACTGC CCTTGGGAGA GGGAACGACT CTCAGGGGTG 
TGGCTTGAGG AAGAGGAGGC GGGGTTTGTT AAAGAATCTT ATGAAGGATG CAGCGACGAC 
CCTGATATAG ACGTCAGCTC ACTCAGTGAG TCCAAGCTAT GTGACCAACG GGAACAGGAG 
GAGTCTGTTC CTTTCCACCA ATCCCCTGCC TCTACAGAAT CTCCAGTCTC AATGAAACCT 
TCAGAATCAG ACCTCACACC TGATCCGTCA ACAACCCCCG AGAGCTCGCC TGCCAAGAGG 
TCAGAGGTGG TGGAGGAGTT CTGGATGAAG AGTGCAGAGA TCCGAAAGAG TTTAGGCCTG 
ACTCCGCTTT CTAGAGAATT GCAGCCAAAA CATATAGCAG CTTCAACCCA GACATCTAAT 
GTTAAAGAAA GCTTTTACAC CTCAGTCACA TATAACACCG CTGCTTTGGA TTCTCCAAAC 
CAAAGTGCTA GAATCTGTGA CTCCAGCACA CAAACTCATT CTGTGACAGA TCTTCAAGAA 
ACCTCCCCTC TTGGACCTAC AGATGGCGAT GCTGGCGTCG ATTTAGGACG TTGTTCTGTT 
GTTCACAGAC TCAGCATCAC TGTAGAAGGT TGTGTGATGG CAGATAACCA GGGTTTGGAT 
TCTACCTCGT TTGCTACTGA AAGTGTTTTA AACCCAGACG CAGGTCTTCC CACCCCTCCT 
TATAGTCCGT CTCACTCCCC TCTTGTTGGT AAACAGTGTC GTGCCCTTCA CCATTCTGAG 
CCAATTCTAG ACAGAGAGGT TATGGCTTTC TCATCTACCT GCACTGCTTC TGTACCACAG 
CGGAGCAGTT TTAAATCAGA CCTCGAGCAA GCTCAAAGTC TTCCACCAGA TGAAATCGAG 
ATCCTGTGCG GAGACGAGGC GGAAAAACTC CCGGAAAGAT CTTGCAGGAT GGAGAGCGGA 
GAGGTGGATA ACCGTAGAGC GGAGCACAGC AGGACGCTTC CTGACCGGGT GGTCGCACCG 
CTGCTGGCAG GGGGACCAGA GGTCAGGCTT CGCAGGTCTG AGATGAAGTT GTGGGGTCCA 
GACGCAGATG GTGATGTGAA GGAGAAAAAG CGCAGCTCGT TGTTTTCACC CCGTAAAAGC 
AGGAAGAATG GAAATGCAGC AGCTGAGAGC GGGCGAGAGA CAGGGAAGCA CAAGTCCCTC 
TGGAAGACTG TTTTTTCAGT GTATAAGAAG GATAAAAAGA GGAAGGAGGT GGCAGTGGTG 
GCGGAAACAC TTCCTGCTGC TAATAATGGG AGCAAACGGA AAGTGTCGGG AATTAACAGA 
ACCACAGACC TGTGTTTCCG GAAGAATCCA AGTTTCTCTG AAGATACGGA TATGTCCTGT 
CATGCTCTTC TTGAAAGATG TCCTCTCCGA GCTCAGAGAG CAGGAACAGA GGAGGAACTC 
AATGCCAGGC TGACACGTCG AGTGCAGAGA GCAGCGCGCA GACAGGCCAA GCAGGAGGAG 
CTGCGGAGGC TGCATAGAGC TCAGATAATC CAGCGTCAGT TGGAGCAGGT GGAAGTGAAG 
CAGAGGCAGC TGGAGGAGAA AGGTGTGGCT GTGGAGAAAG CACTCAGAGG AGAAGCAGTT 
GAGCCTTTTG ACGGTTCTCC CCGTAGAAGG CCTGTATATC TCTGTCCTTG CTGTTCCTCT 
GAAGGTAAAA CCGGCCCTGC TTCCTTTCTC ACTCCTCTAG TGCAGATTTT AGCAATACTT 
TACAATCCTT TTTACCTTTT CTTCCTCTCT GCTCATTCGT CCTGCCTCCT GATGTGTGCA 
GCCAAAGCTA AAGGAACCGA ACATGATCAT TCGGTTTATG TCTGTACAGT CTTTTTCCTG 
TACGTAGTGC AGGAGCCTCC ACTTCACCAA CTGAGAATGG GAAAGAAAGA CGATCCAAGC 
TTAATGCATC AGTGGTTCAA ACTGGTGCAG GAGAAGAATG CTCTCGTGCG TTATGAATCT 
GAGCTCATGA TATTCGCTCG TGAACTGGAG CTGGAGGACA GACAGAGTCG CCTGCAGCAG 
GAGCTGAGAG AGCGCATGGC GGTGGACGAT CATCTAAAAG GAGAAGAGGA GCTGGCGGAG 
GAGAGGCGTA TATTGAGCGA GATGCTGGAT GTGGTGGAGC AGAGGGATGC ACTGGTGGCC 
CTCCTGGAGG AGCAGAGAGT TCGAGAAAAA GAGGAGGACA GTGACCTGGA GGCTGTGATG 
CTGTCTAAGG GCTTCAGTCT GCATTGGGAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mical3b ( XM_017355281.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210770.1 Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X10, mRNA.

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CloneID	ODa58551
Clone ID Related Accession (Same CDS sequence)	XM_017355281.1
Accession Version	XM_017355281.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein-methionine sulfoxide oxidase mical3b isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTTTAA AGGTGGCCTT GTTATTGGGT GTCGAAGTTC ATGTGAACGT GGAGTTCAAA 
CATCTACTGG AACCTCCAGA GAACCAGGAG AAACGTGTTG GTTGGCGGGC AGAGGTTCAG 
CCCAGCTCTC ATCCAGTCAG ACAGCTGGAG TTTGATGTGG TTATTGGGGC AGACGGCAGA 
AGAAACACAT TACCAGGGTT TCGCAGGAAG GAGTTTCGTG GGAAACTGGC CATTGCCATC 
ACGGCGAATT TCATCAATCG CAACACCACC GCCGAGGCTA AAGTGGAGGA GATCAGCGGC 
GTCGCCTTCA TTTTCAACCA GAGGTTTTTC CAGGACCTCC GGCAAGCTAC CGGAATCGAT 
CTGGAAAATA TAGTATACTA TAAAGACGAC ACTCACTATT TCGTCATGAC CGCCAAAAAA 
CAAAGTCTTC TGGAGAAAGG AGTCATCCTG CGTGATTATG CTGACACTGA GATGCTGCTG 
TCCAGACATA ATGTGGACCA GAATGCACTG CTCTCGTATG CACGTGAAGC GGCTGATTTC 
TCCACCAATC ATCAGCTTCC AGCGCTGGAT TTTGCCATCA ATCACTATGG GCAGTCAGAT 
GTGGCCATGT TTGACTTTAC CTGTATGTAC GCCTCGGAAA ACGCAGCGAT GGTACGTCAG 
CGTATGGGAC ATCCACTGCT CGTTGCTCTG GTGGGCGACA GTTTGCTGGA GCCGTTCTGG 
CCAATGGGAA CAGGGATAGC TCGGGGATTC CTGGCAGCGA TGGACACAGC GTGGATGGTG 
CGCAGCTGGG GTCAGGGCAA CACTCCTCTG GAGGTTCTGG CTGAGAGAGA GAGTGTGTAT 
AGGTTGCTTC CTCAGACAAC TCCAGAAAAT GTCAGTAAAA ACTACAGCCA GTTCAGTGTG 
GATCCTGCTA GTCGCTATCC CAACATTAAT ATGCAGCTCA TCAACGCCGC TCAGGTGCGT 
CACCTCATAG ACACAGGTGA GGGTCCAGTT TTGGGTCTCG ATGCTGTTAG TTCTCCACAC 
CCAAGACTTA CGCGACAAGA GTCAATGGCT CGGTACAGTA AACTGCTGAG CTGGTGTCAG 
GAACACACTC ATGGTTACAG GAAAGTGTGT GTGACGGACT TCACCTCTTC CTGGAGGAGC 
GGACTGGCCC TGTGTGCTCT CATTCACACC TTCAGGCCTG ACCTCATTGA TTTTGCATCT 
CTGGAGGAGA GCGAGGCAGA GTTCAGTGGT CAGCTGGGTT TGGACGTGGC GGAGCAGGAA 
TTTGGTATTT GTCCTATAAT GACCGGCAAG GAGATGAGTG TGCTGGAAGA GAGCGACTCT 
CTCTGTATGG TCATGTACCT CAGTCAGCTT CATGAGCTCC TCAAAGACAC ATCGCCACCT 
AGTGGAAGTC AGAGCTCAGA AGAGAGAGCG GTTTTGTTCT CCAGCAGCAG ATCACCAATC 
TCACTGCTCA GCAAACTGGG ACAGAGTTTG TCCCGCAAAC GCAATCCTAA GGATAAGAAA 
GAGAAGGAAG CAGACAGTGT TGGAAAGAGG AGGAGAACAA GTCAGGCCGG CCAATCGGAA 
GAGGAGGATG CTCTTCATGA CGGCAATGAA AATAAGTCTC CAGCAGAAAC TCCCGGCTCA 
GAACCGAAGG CCTCTGAGGG TCACAGCAAG GTTCGGTCCA TGGCGTCGCT CCTTCTGGCT 
AAATTTGAGG AGAACTCGCC ATCGCCTTCA ACCACTGCAA TACGCAGACA GAACTACATT 
CACATGTATA CCGGCGGAGT GAGCTCATTG GCTCTGCAGA TAGCCAATCA GATTCAAAGT 
CAGCAGGCTC AGGCTCCCAA GCTCCTTCAC AGGAGGGAGT CGGGTTCTCA GAAGGATCTT 
CCAGTAAATG TCGGCAGTAG TGATGTGTGT TATTTCTGTG GCCGTCGCGT TTATGTCATG 
GAGAGGCTGA GCGCAGAGGG AAAGTTCTTC CACAGGAGCT GCTTCCAGTG CGACCACTGC 
AGCTCCACGA TACGCCTCTC CAACTACACT TATGACCAGC TACATGGCAA GTTTTACTGT 
AAGCATCACT TCAGCTTCAG ACTGGCAAGT GTGGCTCAGA GAAAGAGACC GGCCCCACCG 
GTGGCCCCTC GGCCTGCTCA GGCTTCCCTG GCTGCATCCT CGGCCTCTAC CTCTTTGTCC 
TCTTTGGGCT CAGTGGGCAC AGCCACACCA GACAGCTGGT CCTCCAGCAC CCACACAGAC 
ATGGCATCCA GCTTGGCCAA GAGACTGTGC GGCACACCAG AACGCATTGA GCTTGAGAAT 
TACAAACCCT CCCCACAGAA ACAAGACAGC CCCCTGCAGG AGGTACCAGA GGAAACCCTC 
GCACAGCACA ATCTCAGCGC TAGCCTGCAG GAGAAGAATG CTGAAGAGCA GTCCAGTAGC 
TCTGAGTCAG ATCTAGAGGA GGAGGAGCTT GTCTGGAAGA AGGGGGAGGA GCTACATGCC 
AGGACCAATG GAGAGAGGAA GCTTGACCTA GAGGAGGAGC TGAAGGAAGA AGAGGGAGGG 
GAGAAGCTGG AAAAACAAGA GGGGGAGGAA GAAGGAGAAG TTTCAGAAGA AGAGCAAGAC 
GAGGGAGATT CTAGTGATGA GGGTGAGTAC TGCCCTTGGG AGAGGGAACG ACTCTCAGGG 
GTGTGGCTTG AGGAAGAGGA GGCGGGGTTT GTTAAAGAAT CTTATGAAGG ATGCAGCGAC 
GACCCTGATA TAGACGTCAG CTCACTCAGT GAGTCCAAGC TATGTGACCA ACGGGAACAG 
GAGGAGTCTG TTCCTTTCCA CCAATCCCCT GCCTCTACAG AATCTCCAGT CTCAATGAAA 
CCTTCAGAAT CAGACCTCAC ACCTGATCCG TCAACAACCC CCGAGAGCTC GCCTGCCAAG 
AGGTCAGAGG TGGTGGAGGA GTTCTGGATG AAGAGTGCAG AGATCCGAAA GAGTTTAGGC 
CTGACTCCGC TTTCTAGAGA ATTGCAGCCA AAACATATAG CAGCTTCAAC CCAGACATCT 
AATGTTAAAG AAAGCTTTTA CACCTCAGTC ACATATAACA CCGCTGCTTT GGATTCTCCA 
AACCAAAGTG CTAGAATCTG TGACTCCAGC ACACAAACTC ATTCTGTGAC AGATCTTCAA 
GAAACCTCCC CTCTTGGACC TACAGATGGC GATGCTGGCG TCGATTTAGG ACGTTGTTCT 
GTTGTTCACA GACTCAGCAT CACTGTAGAA GGTTGTGTGA TGGCAGATAA CCAGGGTTTG 
GATTCTACCT CGTTTGCTAC TGAAAGTGTT TTAAACCCAG ACGCAGGTCT TCCCACCCCT 
CCTTATAGTC CGTCTCACTC CCCTCTTGTT GGTAAACAGT GTCGTGCCCT TCACCATTCT 
GAGCCAATTC TAGACAGAGA GGTTATGGCT TTCTCATCTA CCTGCACTGC TTCTGTACCA 
CAGCGGAGCA GTTTTAAATC AGACCTCGAG CAAGCTCAAA GTCTTCCACC AGATGAAATC 
GAGATCCTGT GCGGAGACGA GGCGGAAAAA CTCCCGGAAA GATCTTGCAG GATGGAGAGC 
GGAGAGGTGG ATAACCGTAG AGCGGAGCAC AGCAGGACGC TTCCTGACCG GGTGGTCGCA 
CCGCTGCTGG CAGGGGGACC AGAGGTCAGG CTTCGCAGGT CTGAGATGAA GTTGTGGGGT 
CCAGACGCAG ATGGTGATGT GAAGGAGAAA AAGCGCAGCT CGTTGTTTTC ACCCCGTAAA 
AGCAGGAAGA ATGGAAATGC AGCAGCTGAG AGCGGGCGAG AGACAGGGAA GCACAAGTCC 
CTCTGGAAGA CTGTTTTTTC AGTGTATAAG AAGGATAAAA AGAGGAAGGA GGTGGCAGTG 
GTGGCGGAAA CACTTCCTGC TGCTAATAAT GGGAGCAAAC GGAAAGTGTC GGGAATTAAC 
AGAACCACAG ACCTGTGTTT CCGGAAGAAT CCAAGTTTCT CTGAAGATAC GGATATGTCC 
TGTCATGCTC TTCTTGAAAG ATGTCCTCTC CGAGCTCAGA GAGCAGGAAC AGAGGAGGAA 
CTCAATGCCA GGCTGACACG TCGAGTGCAG AGAGCAGCGC GCAGACAGGC CAAGCAGGAG 
GAGCTGCGGA GGCTGCATAG AGCTCAGATA ATCCAGCGTC AGTTGGAGCA GGTGGAAGTG 
AAGCAGAGGC AGCTGGAGGA GAAAGGTGTG GCTGTGGAGA AAGCACTCAG AGGAGAAGCA 
GTTGAGCCTT TTGACGGTTC TCCCCGTAGA AGGCCTGTAT ATCTCTGTCC TTGCTGTTCC 
TCTGAAGGTA AAACCGGCCC TGCTTCCTTT CTCACTCCTC TAGTGCAGAT TTTAGCAATA 
CTTTACAATC CTTTTTACCT TTTCTTCCTC TCTGCTCATT CGTCCTGCCT CCTGATGTGT 
GCAGCCAAAG CTAAAGGAAC CGAACATGAT CATTCGGTTT ATGTCTGTAC AGTCTTTTTC 
CTGTACGTAG TGCAGGAGCC TCCACTTCAC CAACTGAGAA TGGGAAAGAA AGACGATCCA 
AGCTTAATGC ATCAGTGGTT CAAACTGGTG CAGGAGAAGA ATGCTCTCGT GCGTTATGAA 
TCTGAGCTCA TGATATTCGC TCGTGAACTG GAGCTGGAGG ACAGACAGAG TCGCCTGCAG 
CAGGAGCTGA GAGAGCGCAT GGCGGTGGAC GATCATCTAA AAGGAGAAGA GGAGCTGGCG 
GAGGAGAGGC GTATATTGAG CGAGATGCTG GATGTGGTGG AGCAGAGGGA TGCACTGGTG 
GCCCTCCTGG AGGAGCAGAG AGTTCGAGAA AAAGAGGAGG ACAGTGACCT GGAGGCTGTG 
ATGCTGTCTA AGGGCTTCAG TCTGCATTGG GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210770.1
CDS	78..4913
Translation	MLLKVALLLGVEVHVNVEFKHLLEPPENQEKRVGWRAEVQPSSHPVRQLEFDVVIGADGRRNTLPGFRRKEFRGKLAIAITANFINRNTTAEAKVEEISGVAFIFNQRFFQDLRQATGIDLENIVYYKDDTHYFVMTAKKQSLLEKGVILRDYADTEMLLSRHNVDQNALLSYAREAADFSTNHQLPALDFAINHYGQSDVAMFDFTCMYASENAAMVRQRMGHPLLVALVGDSLLEPFWPMGTGIARGFLAAMDTAWMVRSWGQGNTPLEVLAERESVYRLLPQTTPENVSKNYSQFSVDPASRYPNINMQLINAAQVRHLIDTGEGPVLGLDAVSSPHPRLTRQESMARYSKLLSWCQEHTHGYRKVCVTDFTSSWRSGLALCALIHTFRPDLIDFASLEESEAEFSGQLGLDVAEQEFGICPIMTGKEMSVLEESDSLCMVMYLSQLHELLKDTSPPSGSQSSEERAVLFSSSRSPISLLSKLGQSLSRKRNPKDKKEKEADSVGKRRRTSQAGQSEEEDALHDGNENKSPAETPGSEPKASEGHSKVRSMASLLLAKFEENSPSPSTTAIRRQNYIHMYTGGVSSLALQIANQIQSQQAQAPKLLHRRESGSQKDLPVNVGSSDVCYFCGRRVYVMERLSAEGKFFHRSCFQCDHCSSTIRLSNYTYDQLHGKFYCKHHFSFRLASVAQRKRPAPPVAPRPAQASLAASSASTSLSSLGSVGTATPDSWSSSTHTDMASSLAKRLCGTPERIELENYKPSPQKQDSPLQEVPEETLAQHNLSASLQEKNAEEQSSSSESDLEEEELVWKKGEELHARTNGERKLDLEEELKEEEGGEKLEKQEGEEEGEVSEEEQDEGDSSDEGEYCPWERERLSGVWLEEEEAGFVKESYEGCSDDPDIDVSSLSESKLCDQREQEESVPFHQSPASTESPVSMKPSESDLTPDPSTTPESSPAKRSEVVEEFWMKSAEIRKSLGLTPLSRELQPKHIAASTQTSNVKESFYTSVTYNTAALDSPNQSARICDSSTQTHSVTDLQETSPLGPTDGDAGVDLGRCSVVHRLSITVEGCVMADNQGLDSTSFATESVLNPDAGLPTPPYSPSHSPLVGKQCRALHHSEPILDREVMAFSSTCTASVPQRSSFKSDLEQAQSLPPDEIEILCGDEAEKLPERSCRMESGEVDNRRAEHSRTLPDRVVAPLLAGGPEVRLRRSEMKLWGPDADGDVKEKKRSSLFSPRKSRKNGNAAAESGRETGKHKSLWKTVFSVYKKDKKRKEVAVVAETLPAANNGSKRKVSGINRTTDLCFRKNPSFSEDTDMSCHALLERCPLRAQRAGTEEELNARLTRRVQRAARRQAKQEELRRLHRAQIIQRQLEQVEVKQRQLEEKGVAVEKALRGEAVEPFDGSPRRRPVYLCPCCSSEGKTGPASFLTPLVQILAILYNPFYLFFLSAHSSCLLMCAAKAKGTEHDHSVYVCTVFFLYVVQEPPLHQLRMGKKDDPSLMHQWFKLVQEKNALVRYESELMIFARELELEDRQSRLQQELRERMAVDDHLKGEEELAEERRILSEMLDVVEQRDALVALLEEQRVREKEEDSDLEAVMLSKGFSLHWD

Target ORF information:

RefSeq Version	XM_017355281.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio microtubule associated monooxygenase, calponin and LIM domain containing 3b (mical3b), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017355281.1

ORF Insert Sequence:

ATGCTTTTAA AGGTGGCCTT GTTATTGGGT GTCGAAGTTC ATGTGAACGT GGAGTTCAAA 
CATCTACTGG AACCTCCAGA GAACCAGGAG AAACGTGTTG GTTGGCGGGC AGAGGTTCAG 
CCCAGCTCTC ATCCAGTCAG ACAGCTGGAG TTTGATGTGG TTATTGGGGC AGACGGCAGA 
AGAAACACAT TACCAGGGTT TCGCAGGAAG GAGTTTCGTG GGAAACTGGC CATTGCCATC 
ACGGCGAATT TCATCAATCG CAACACCACC GCCGAGGCTA AAGTGGAGGA GATCAGCGGC 
GTCGCCTTCA TTTTCAACCA GAGGTTTTTC CAGGACCTCC GGCAAGCTAC CGGAATCGAT 
CTGGAAAATA TAGTATACTA TAAAGACGAC ACTCACTATT TCGTCATGAC CGCCAAAAAA 
CAAAGTCTTC TGGAGAAAGG AGTCATCCTG CGTGATTATG CTGACACTGA GATGCTGCTG 
TCCAGACATA ATGTGGACCA GAATGCACTG CTCTCGTATG CACGTGAAGC GGCTGATTTC 
TCCACCAATC ATCAGCTTCC AGCGCTGGAT TTTGCCATCA ATCACTATGG GCAGTCAGAT 
GTGGCCATGT TTGACTTTAC CTGTATGTAC GCCTCGGAAA ACGCAGCGAT GGTACGTCAG 
CGTATGGGAC ATCCACTGCT CGTTGCTCTG GTGGGCGACA GTTTGCTGGA GCCGTTCTGG 
CCAATGGGAA CAGGGATAGC TCGGGGATTC CTGGCAGCGA TGGACACAGC GTGGATGGTG 
CGCAGCTGGG GTCAGGGCAA CACTCCTCTG GAGGTTCTGG CTGAGAGAGA GAGTGTGTAT 
AGGTTGCTTC CTCAGACAAC TCCAGAAAAT GTCAGTAAAA ACTACAGCCA GTTCAGTGTG 
GATCCTGCTA GTCGCTATCC CAACATTAAT ATGCAGCTCA TCAACGCCGC TCAGGTGCGT 
CACCTCATAG ACACAGGTGA GGGTCCAGTT TTGGGTCTCG ATGCTGTTAG TTCTCCACAC 
CCAAGACTTA CGCGACAAGA GTCAATGGCT CGGTACAGTA AACTGCTGAG CTGGTGTCAG 
GAACACACTC ATGGTTACAG GAAAGTGTGT GTGACGGACT TCACCTCTTC CTGGAGGAGC 
GGACTGGCCC TGTGTGCTCT CATTCACACC TTCAGGCCTG ACCTCATTGA TTTTGCATCT 
CTGGAGGAGA GCGAGGCAGA GTTCAGTGGT CAGCTGGGTT TGGACGTGGC GGAGCAGGAA 
TTTGGTATTT GTCCTATAAT GACCGGCAAG GAGATGAGTG TGCTGGAAGA GAGCGACTCT 
CTCTGTATGG TCATGTACCT CAGTCAGCTT CATGAGCTCC TCAAAGACAC ATCGCCACCT 
AGTGGAAGTC AGAGCTCAGA AGAGAGAGCG GTTTTGTTCT CCAGCAGCAG ATCACCAATC 
TCACTGCTCA GCAAACTGGG ACAGAGTTTG TCCCGCAAAC GCAATCCTAA GGATAAGAAA 
GAGAAGGAAG CAGACAGTGT TGGAAAGAGG AGGAGAACAA GTCAGGCCGG CCAATCGGAA 
GAGGAGGATG CTCTTCATGA CGGCAATGAA AATAAGTCTC CAGCAGAAAC TCCCGGCTCA 
GAACCGAAGG CCTCTGAGGG TCACAGCAAG GTTCGGTCCA TGGCGTCGCT CCTTCTGGCT 
AAATTTGAGG AGAACTCGCC ATCGCCTTCA ACCACTGCAA TACGCAGACA GAACTACATT 
CACATGTATA CCGGCGGAGT GAGCTCATTG GCTCTGCAGA TAGCCAATCA GATTCAAAGT 
CAGCAGGCTC AGGCTCCCAA GCTCCTTCAC AGGAGGGAGT CGGGTTCTCA GAAGGATCTT 
CCAGTAAATG TCGGCAGTAG TGATGTGTGT TATTTCTGTG GCCGTCGCGT TTATGTCATG 
GAGAGGCTGA GCGCAGAGGG AAAGTTCTTC CACAGGAGCT GCTTCCAGTG CGACCACTGC 
AGCTCCACGA TACGCCTCTC CAACTACACT TATGACCAGC TACATGGCAA GTTTTACTGT 
AAGCATCACT TCAGCTTCAG ACTGGCAAGT GTGGCTCAGA GAAAGAGACC GGCCCCACCG 
GTGGCCCCTC GGCCTGCTCA GGCTTCCCTG GCTGCATCCT CGGCCTCTAC CTCTTTGTCC 
TCTTTGGGCT CAGTGGGCAC AGCCACACCA GACAGCTGGT CCTCCAGCAC CCACACAGAC 
ATGGCATCCA GCTTGGCCAA GAGACTGTGC GGCACACCAG AACGCATTGA GCTTGAGAAT 
TACAAACCCT CCCCACAGAA ACAAGACAGC CCCCTGCAGG AGGTACCAGA GGAAACCCTC 
GCACAGCACA ATCTCAGCGC TAGCCTGCAG GAGAAGAATG CTGAAGAGCA GTCCAGTAGC 
TCTGAGTCAG ATCTAGAGGA GGAGGAGCTT GTCTGGAAGA AGGGGGAGGA GCTACATGCC 
AGGACCAATG GAGAGAGGAA GCTTGACCTA GAGGAGGAGC TGAAGGAAGA AGAGGGAGGG 
GAGAAGCTGG AAAAACAAGA GGGGGAGGAA GAAGGAGAAG TTTCAGAAGA AGAGCAAGAC 
GAGGGAGATT CTAGTGATGA GGGTGAGTAC TGCCCTTGGG AGAGGGAACG ACTCTCAGGG 
GTGTGGCTTG AGGAAGAGGA GGCGGGGTTT GTTAAAGAAT CTTATGAAGG ATGCAGCGAC 
GACCCTGATA TAGACGTCAG CTCACTCAGT GAGTCCAAGC TATGTGACCA ACGGGAACAG 
GAGGAGTCTG TTCCTTTCCA CCAATCCCCT GCCTCTACAG AATCTCCAGT CTCAATGAAA 
CCTTCAGAAT CAGACCTCAC ACCTGATCCG TCAACAACCC CCGAGAGCTC GCCTGCCAAG 
AGGTCAGAGG TGGTGGAGGA GTTCTGGATG AAGAGTGCAG AGATCCGAAA GAGTTTAGGC 
CTGACTCCGC TTTCTAGAGA ATTGCAGCCA AAACATATAG CAGCTTCAAC CCAGACATCT 
AATGTTAAAG AAAGCTTTTA CACCTCAGTC ACATATAACA CCGCTGCTTT GGATTCTCCA 
AACCAAAGTG CTAGAATCTG TGACTCCAGC ACACAAACTC ATTCTGTGAC AGATCTTCAA 
GAAACCTCCC CTCTTGGACC TACAGATGGC GATGCTGGCG TCGATTTAGG ACGTTGTTCT 
GTTGTTCACA GACTCAGCAT CACTGTAGAA GGTTGTGTGA TGGCAGATAA CCAGGGTTTG 
GATTCTACCT CGTTTGCTAC TGAAAGTGTT TTAAACCCAG ACGCAGGTCT TCCCACCCCT 
CCTTATAGTC CGTCTCACTC CCCTCTTGTT GGTAAACAGT GTCGTGCCCT TCACCATTCT 
GAGCCAATTC TAGACAGAGA GGTTATGGCT TTCTCATCTA CCTGCACTGC TTCTGTACCA 
CAGCGGAGCA GTTTTAAATC AGACCTCGAG CAAGCTCAAA GTCTTCCACC AGATGAAATC 
GAGATCCTGT GCGGAGACGA GGCGGAAAAA CTCCCGGAAA GATCTTGCAG GATGGAGAGC 
GGAGAGGTGG ATAACCGTAG AGCGGAGCAC AGCAGGACGC TTCCTGACCG GGTGGTCGCA 
CCGCTGCTGG CAGGGGGACC AGAGGTCAGG CTTCGCAGGT CTGAGATGAA GTTGTGGGGT 
CCAGACGCAG ATGGTGATGT GAAGGAGAAA AAGCGCAGCT CGTTGTTTTC ACCCCGTAAA 
AGCAGGAAGA ATGGAAATGC AGCAGCTGAG AGCGGGCGAG AGACAGGGAA GCACAAGTCC 
CTCTGGAAGA CTGTTTTTTC AGTGTATAAG AAGGATAAAA AGAGGAAGGA GGTGGCAGTG 
GTGGCGGAAA CACTTCCTGC TGCTAATAAT GGGAGCAAAC GGAAAGTGTC GGGAATTAAC 
AGAACCACAG ACCTGTGTTT CCGGAAGAAT CCAAGTTTCT CTGAAGATAC GGATATGTCC 
TGTCATGCTC TTCTTGAAAG ATGTCCTCTC CGAGCTCAGA GAGCAGGAAC AGAGGAGGAA 
CTCAATGCCA GGCTGACACG TCGAGTGCAG AGAGCAGCGC GCAGACAGGC CAAGCAGGAG 
GAGCTGCGGA GGCTGCATAG AGCTCAGATA ATCCAGCGTC AGTTGGAGCA GGTGGAAGTG 
AAGCAGAGGC AGCTGGAGGA GAAAGGTGTG GCTGTGGAGA AAGCACTCAG AGGAGAAGCA 
GTTGAGCCTT TTGACGGTTC TCCCCGTAGA AGGCCTGTAT ATCTCTGTCC TTGCTGTTCC 
TCTGAAGGTA AAACCGGCCC TGCTTCCTTT CTCACTCCTC TAGTGCAGAT TTTAGCAATA 
CTTTACAATC CTTTTTACCT TTTCTTCCTC TCTGCTCATT CGTCCTGCCT CCTGATGTGT 
GCAGCCAAAG CTAAAGGAAC CGAACATGAT CATTCGGTTT ATGTCTGTAC AGTCTTTTTC 
CTGTACGTAG TGCAGGAGCC TCCACTTCAC CAACTGAGAA TGGGAAAGAA AGACGATCCA 
AGCTTAATGC ATCAGTGGTT CAAACTGGTG CAGGAGAAGA ATGCTCTCGT GCGTTATGAA 
TCTGAGCTCA TGATATTCGC TCGTGAACTG GAGCTGGAGG ACAGACAGAG TCGCCTGCAG 
CAGGAGCTGA GAGAGCGCAT GGCGGTGGAC GATCATCTAA AAGGAGAAGA GGAGCTGGCG 
GAGGAGAGGC GTATATTGAG CGAGATGCTG GATGTGGTGG AGCAGAGGGA TGCACTGGTG 
GCCCTCCTGG AGGAGCAGAG AGTTCGAGAA AAAGAGGAGG ACAGTGACCT GGAGGCTGTG 
ATGCTGTCTA AGGGCTTCAG TCTGCATTGG GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Eumetazoan cryptochrome phylogeny and evolution.
Genome biology and evolution7(2)601-19(2015 Jan)
Haug MF,Gesemann M,Lazovi? V,Neuhauss SC

Functional validation of GWAS gene candidates for abnormal liver function during zebrafish liver development.
Disease models & mechanisms6(5)1271-8(2013 Sep)
Liu LY,Fox CS,North TE,Goessling W

Identification and expression analysis of mical family genes in zebrafish.
Journal of genetics and genomics = Yi chuan xue bao37(10)685-93(2010 Oct)
Xue Y,Kuok C,Xiao A,Zhu Z,Lin S,Zhang B

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mical3b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

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