lama4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	lama4
Entrez Gene ID	566796
Full Name	laminin, alpha 4
Synonyms	si:busm1-189a20.2,si:dkey-119m7.3
General protein information	Preferred Names laminin, alpha 4 Names laminin subunit alpha-4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001039065.2	NP_001034154.1	laminin subunit alpha-4 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	LAMA4	NP_001098676.2
	LAMA4	XP_002803983.1
Bos taurus (cattle)	LAMA4	NP_001192894.1
Danio rerio (zebrafish)	lama4	NP_001034154.1
Pan troglodytes (chimpanzee)	LAMA4	XP_518696.3
Canis lupus familiaris (dog)	LAMA4	XP_005627847.1
Rattus norvegicus (Norway rat)	Lama4	XP_003752023.1
Gallus gallus (chicken)	LAMA4	XP_004940355.1
Mus musculus (house mouse)	Lama4	NP_034811.2
Xenopus tropicalis (tropical clawed frog)	lama4	XP_002936983.2

Gene Ontology
Bibliography

Related articles in PubMed

Cardiac transcriptome and dilated cardiomyopathy genes in zebrafish.
Shih YH, Zhang Y, Ding Y, Ross CA, Li H, Olson TM, Xu X
Circulation. Cardiovascular genetics8(2)261-9(2015 Apr)

Epistatic dissection of laminin-receptor interactions in dystrophic zebrafish muscle.
Sztal TE, Sonntag C, Hall TE, Currie PD
Human molecular genetics21(21)4718-31(2012 Nov)

Global identification of SMAD2 target genes reveals a role for multiple co-regulatory factors in zebrafish early gastrulas.
Liu Z, Lin X, Cai Z, Zhang Z, Han C, Jia S, Meng A, Wang Q
The Journal of biological chemistry286(32)28520-32(2011 Aug)

Characterization of the laminin gene family and evolution in zebrafish.
Sztal T, Berger S, Currie PD, Hall TE
Developmental dynamics : an official publication of the American Association of Anatomists240(2)422-31(2011 Feb)

Concomitant duplications of opioid peptide and receptor genes before the origin of jawed vertebrates.
Sundstr?m G, Dreborg S, Larhammar D
PloS one5(5)e10512(2010 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Knockdown of Lama4 in zebrafish resulted inthe same kind of cardiac dysfunction & hemorrhages seen in Ilk mutants. There is epistatic regulation between laminin-alpha4 (Lama4), integrin, and Ilk.
Title: Laminin-alpha4 and integrin-linked kinase mutations cause human cardiomyopathy via simultaneous defects in cardiomyocytes and endothelial cells.

The following lama4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lama4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa34033	NM_001039065.2 Latest version!	Danio rerio laminin, alpha 4 (lama4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

lama4 ( NM_001039065.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001034154.1 Danio rerio laminin, alpha 4 (lama4), mRNA.

Price & Availability↑

CloneID	ODa34033
Clone ID Related Accession (Same CDS sequence)	NM_001039065.2
Accession Version	NM_001039065.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-25
Organism	Danio rerio(zebrafish)
Product	laminin subunit alpha-4 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY862414.1. On May 24, 2007 this sequence version replaced NM_001039065.1. ##Evidence-Data-START## Transcript exon combination :: AY862414.1, BC163717.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTCTC TGCTGTCCGG ATCTGTCTGC TTTCTGTATG TCTTGTCAGC ACTCGTGCCC 
GCAGATTCAA CACAACAGTT CAACACAGTC TACAGGGTGT GTGCTCGAGG GTACTTCTTG 
AGTGATCAGG AGACATGTCT GCCCTGTAAC TGTAAAGGCC ATGCGGATAG CTGTGAGGAC 
ATCACCGGGA TCTGTATTGA TTGTCGGGAC CACACTCTCG GAGATTTCTG TGAGCTGTGT 
GAAGACAGAT TTTTGCCCGT GTTTCTTCCT GATGGCACCT TTATGTGTAG ACCGTGTGCC 
TGCCCGCTGT CACTGGAGTC TAACAACTTT GCAGCTCACT GTGATAGGAG GGGTGAGTTT 
CTGAGATGTG TCTGCCAGGA CGGGTATGCT GGGCACTACT GCGAGAGATG TGCTCCGGGT 
TATTATGGCA ACCCCATGGA GTTGGGCAAT TCTTGCAAAA AGTGTGATTG TAACGGCAAC 
TCTGACCCCA ACCTGATCTT CAACGAGTGC CATAATGTGA CAGGAAAGTG CCTGAACTGC 
TGGGGTGACA CTGCAGGGGA CAACTGTGAA AGGTGTGCAC CTGGTTTCTA TGGCGACGCC 
ATCAGTGCTA AAGACTGTCA AGAGTGCCAG TGCAATAAGT GTGGTACTGC ATCATGTGAT 
GACAGGTCAG GTGTGTGCCA CTGTAAGCCT GGGGTCACAG GTCAGCTGTG TGACAGGTGT 
GAGGACGGTC ATTCTGGATT TTGCACCTGC ATGGGTTGCC GTCGATGTGA ATGTGCTTCT 
GCTGCCCTGC GTGCCACCTG CCACCCTCTA ACACACAGCT GCCAGTGCCG CCCGGGGGCT 
GGAGGTCGAT ACTGCGAGCG CTGCCTGCCG GGATTCTGGG ATTACAGCCC ATCCGGCTGC 
AGGAAATGCG ACTGTCCAGA AGAGAACTGT GACCTGCACA CAGGAGAGTG TCTGCCTGAG 
CTCTCGCAAA TCAGCGAGTG TAATATCAGC TGTGACGCGT GTATCTGGAA TCTGATCGGA 
GACATTCGCC AGTCCAACAA AACTCTGGAC CAGGTGAGGA ACAGCGTGCT GAACATTTCC 
ACAGGAGCTG CGGCTAATGA CAGACTGAAG TACTACAACT ACACTGCGCA CCGGCTACAG 
GCTCAGTTTA GGGGCTGGAG AAAGAATTAT GCCATGATGA GTGGAGATAC AGATGTCCTG 
GAAGAAGAAA CTCAAAATCT GCTGACTGAC ATGGACCAGT TCGCAGAAGA GGAGAATGAA 
GTGCAGAATT TGGGAAAGCA AGTGGATCAA GAGACACTGA ACAGCTCCAC ACGAGCAAAG 
ACCCTCACTG CTAACCTGTC ATCGCTCAAT GTCCTCATTG AAGATATGAT CAGGGACTGG 
GAATTATACG GTGTCTATCA GGAGTTGGAT CCTCAGGTGA AGTTGCAGAA ACTGGCTGAA 
GCTGAACGGA TCTTGGGCTG GATGAGAAAA CTGAACCTGT CACCCAAAGA ACCGCTTGCT 
ACAGATGAGT CCTCCGAGGC GCATGAGTTG CTCAGACGGG TGCGCAGCTT TGACAGAAAT 
CTGATGACTA CACAGGGCCG TCTGCCACCT ATGCAGGAAG TTCTGGGTCG GTTTACTTCT 
AAACTCCTTC AAGCTCAGGA TCTCCTTAGC AAAGCTGAAG ACGCACTGAA ACAGGCCATC 
AACAAATACA AGAGCAACCA GCTCAAACTA CAGAGGAGAG AGGCACAGCA GCAACGTCTG 
AAAGACATCC AGGACGGCGT CGATCAAACA ATTGTTAATG CTGCGGCAGT TCTCTCTGAG 
GCAAATCACA CTGTTCTTGA GGTGGAAGAT GTGATCACGA ATGTGAGAGA ACAGCATGCA 
GAAATTGACG GAGCCCGTGT CTTACTGGAG AGAAGGACAG AGAAGATATC TCAGTCAGAC 
AGAGAACTAG TTCTGCGAGC CGAGGATCAT GCCGATCAAC TGCAGAGACA CGCTGAAGAG 
CCGCAGCATA ATCTGAAAGG AAGCGATGCG AACGGACATG TGCAGAAAGC CCTGAACGCC 
TTCAACGTGT ACGACAATAT TGTCAAGAGC GTAGACGATG CTAACATCAC TAGCCTGACC 
GCCCTCAACA TCTCCAGCAG GACTCACGGT GCGGTCGATA AGCTAAGCTC TCAGCTGAAC 
CTCATGCGCT CTCAGAGTGC AAAAGTGTTG AATGAAGCAT CGTCCCTACA CTTTCAGCAG 
CAAGACAGTG AATCTATCGT ACTCGACAAT AAGAAATACA TTGAAGAGAC CAATGAAATG 
ATGGACGGCT CCTCTAAAAA AATAGCTAAG ATTATCACGG AGATCAATGC AATTCACACA 
GATCGTACCC CGAAGCGTCT GCAGTTCACT CAGGAAGTGG CTGAGGGCAC TCTGAACCGC 
TCCGCTGAGG TGTTGGAGAA AGTGACTCCC ATTCTGAAGA GAGTGGAGCA GTGGGACGGC 
AACATGAAGA GTGAAGCCTA TTCTGCTGCA GCGTTCGACC GCACTGTTCT GTCTGCTGGC 
GAGGCGGTGA AGGACCTGTC AGCCATTGTG CCAGAGCTGC TCAGCAAGCT GCGTGTGGTT 
GAGGAGAAAA AACCTGTGAA TAACGTCACC ACGAGCATCA TGAAAATCAG AGGGCTCATC 
GCACAGGCCC GAAGCGTTGC AAAGAAGGTG CAAGTGTCCA TGAAGTTTGA TGGCCAGTCT 
GCTGTAGAAG TTCATCCCCA CACCAACCTG GATGAGCTGA AGACGGTGAC GTCCATCAGT 
TTCTACATCC GAGTAGACCC AGATAAAGAC CCCATAGAGG ATCGCTTCTT GCTTTACCTC 
GGAGACAAAC AAGGAAAGAG GGACTACATG GGACTCGCCA TAAAGAACGA TAATCTGGTG 
TACGTCTACA AACTGGGTGA AGAGGAAGTG GAGATTCCAC TCAGCTCCAA GCCAGTCAGC 
TCCTGGCCTC CTGTCTTCAA CTTTGTTAAA GTGGAGAGGC TGGGACGCCA TGGCAAAGTG 
TTCCTGACGG TTCCCAGTCA AGACACCACA GCCGAGCAGA AGTTCATCCA GAAAGGAGAG 
CCAGTCGGGA CAGACACGCT GTTTGACCTG GACCCTAAAG ACATGGTGTT CATAGTTGGA 
GGAGTGCCTC CTGGTGTCAC GGTTCCTCCG ATTTTGAGCC TGGCTCCTTT CGTTGGCTGC 
ATTGAGTTAG AGACCCTGAA TAATGATGTC ATCAGTTTGT ACAACTTTAA GCAACTTCAC 
AAAATGGATG TTAAAGCATC GGCGCCTTGT CCCAGGCACA AGCTGGCTTT CTCTCAGAGT 
CGTGTCACCA GTTATTTGTT TGACGGCACC GGCTACGCTT TAGTCAACAA CATTGAGAGG 
CGAGGAAAGA TTGGCGTCGT CACTCGATTT GATATTGAAG TTCGAACTGT TGCTAACAAC 
GGCATCCTCT TCCTCATGGT GAATGAGACC AATTTCTTTG TGCTGGAGCT GAAAAATGGC 
TTTCTCCGTC TGATGTACGA CTTTGGTTTC GCTAACGGTC CTGTCATCAT AGAGACTAAC 
TTAGCCAAAC TGCAGATCAA TGACGCTCGC TATCACGAGG TGTCTGTGAT CTATCATCAT 
TCAAAGAAGA TCATTTTGTT GGTGGACAGA AGCCATGTCA AGTCCTTTGA AAGTGAAAAG 
AAACCCCTAC CCTTTTCTGA TATCTATATA GGAGGAGCTC CTTCGAGAAT TCTGCAGTCT 
AGGCCAGAGC TGACATCCTT GATTGGATTG AAGGGTTGTG TAAAAGGCTT CCAGTTCCAG 
AAAGAGGATT TCAACCTCTT GGAGGAGCAA GGAACCATTG GTATCAGCAG CGGCTGTCCT 
GAGGAATCGT TTATGTCCCA TAAGGCGTAT TTCACTGGCA ATGGTTACCT GAGCTCTTCA 
GATAAAATCT CTCCATTCCA ATCATTTGAA GGAGGACTGA GCTTCAAAAC ACTACAGCCC 
AGCGGGCTCC TGTTCTACCA CAGAGATGGG TCGGATGAGT TTTCTCTTTC TCTGGAGAAC 
GGTGCTGTGG TACTCCAGGC CAAAGGAACT AGAGTGAAAT CCCAGAAGAA GCATTACAGT 
GATGGCCGCT CTCATTTCTT AGTCGCCTCT GTCACCAATC AGAAATATGA AATTGTCATT 
GATGACAAAG ACAAGCAGGA TAAGAAGAAG CCGTCTTCCA CGTCTCAGTC AGACAACACT 
CCAAAGGCCT TCTACTATGG AGGCTCTACA TCCAGTTCCA TACTCAATTT AACTGGGTGC 
ATCAGTTATG CCTACATCAG CAGGCAGGAC AGAGATATAG AGCCAGAAGA TTTCCAGCGC 
TACAGTGAGA ATGTGCAGGC TTCACTACAG GACTGCCCGA TTGAAAGACC CCCGTCTGCT 
CTGCTCAAAG ACAGCAGAGA CTCCTCTAGA AGAAGTGCAG GGCACTCCCG CAAGGTGGGC 
AGGGATGAGT CCGGCCTGCC ACATGGCCTG AAGAGCAATC CTCTGAACAT TCCGGAGAAA 
GAGGAGGCGT CCTGTTACCT GTCGTCCCGC CCACGAGCAA CCCCCCATGC AAATCACTAT 
GGTGGATCTG CCCACAGCAG ACATGAGTAC ACGGGCATCG CCAGTGCCAT CAAACATGAA 
TCTCACTTCT CTCTGTCTCT GAAAACCCGT TCAGCGTTTG GTCTGATCTT CTACCTCGCC 
GACGCGTCTG GGGAGAACTC AATGGCTCTG TTCCTTACTC ACGCAAGACT GGTATTCACG 
TTCAGCTCGG GCCAAAGCCA AGTCAGAATC CGGTCCAAGG AGAAGTACAA TGATGGACAG 
TGGCATGATA TTATCTTTAT CAGAGCTGGA AATATGGGCA GAATGCTAAT CGATGGTCTT 
ACGGTATTAG AGGACAGAGC TCCAGGCAGA AACACCTCGC TGCTTGTCCA GGACCCTCTC 
TATGTTGGCG GAGTCCCACC TCATCAGGCC ATGAAAAACA TTCAGAGGAC ATCTGTGTCG 
AGTTTCACTG GCTGTGTGCG GAGTCTCCAG CTGAATGGCC GCTCGCTCAC CTCTGCCTCC 
CACAGTTTTG GGGTCACCCC CTGCTACGAG GGCCCCTCGG AACCGGGGAC GTACTTCGCT 
GAGGAGGGAG GATATGTGCT GCTGGATGAC TCCTTTCATT TCGGCTCAAG CTTTAAGATG 
GCGATGGAGG TTCGCCCCCG TGTGGCTTCA GGGGTGCTAC TGCATGTCTT CATGGAGCAG 
AAGGAATATC TCACCGTGTA CATCTACCAG AGCCAGGTGG TGGTTGTGGT GAATAACGGT 
ATCGCGGAGT TCTCCACCCA CGTCTCTCCC ACACAAGAAA TCTGTGATGG AAACTGGCAC 
AAGATCACAG TGATCAGAGA TGGTAGCGTG CTTCAGCTGG ATGTGGACTC AGAGGTGAAT 
CACGTTGTGG GGTCTGTCAG TGACGCAGCC CAGAGCAGCA GTTCGCCAGT GTTTATCGGT 
GGAGCTCCAG ACTCCATGCT GTCCCACAGT CTGCTCAGCA GGAGAGGCTT CACGGGCTGC 
ATGAGGAGCG TGTCTGTAAA CGAGACTCCT GTGAGCTTCA GCAAAGCTGT GCTGGTCAGT 
GGATCAGTCG GGGTGGGCGC CTGTCCTTCT GCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034154.1
CDS	313..5928
Translation	MASLLSGSVCFLYVLSALVPADSTQQFNTVYRVCARGYFLSDQETCLPCNCKGHADSCEDITGICIDCRDHTLGDFCELCEDRFLPVFLPDGTFMCRPCACPLSLESNNFAAHCDRRGEFLRCVCQDGYAGHYCERCAPGYYGNPMELGNSCKKCDCNGNSDPNLIFNECHNVTGKCLNCWGDTAGDNCERCAPGFYGDAISAKDCQECQCNKCGTASCDDRSGVCHCKPGVTGQLCDRCEDGHSGFCTCMGCRRCECASAALRATCHPLTHSCQCRPGAGGRYCERCLPGFWDYSPSGCRKCDCPEENCDLHTGECLPELSQISECNISCDACIWNLIGDIRQSNKTLDQVRNSVLNISTGAAANDRLKYYNYTAHRLQAQFRGWRKNYAMMSGDTDVLEEETQNLLTDMDQFAEEENEVQNLGKQVDQETLNSSTRAKTLTANLSSLNVLIEDMIRDWELYGVYQELDPQVKLQKLAEAERILGWMRKLNLSPKEPLATDESSEAHELLRRVRSFDRNLMTTQGRLPPMQEVLGRFTSKLLQAQDLLSKAEDALKQAINKYKSNQLKLQRREAQQQRLKDIQDGVDQTIVNAAAVLSEANHTVLEVEDVITNVREQHAEIDGARVLLERRTEKISQSDRELVLRAEDHADQLQRHAEEPQHNLKGSDANGHVQKALNAFNVYDNIVKSVDDANITSLTALNISSRTHGAVDKLSSQLNLMRSQSAKVLNEASSLHFQQQDSESIVLDNKKYIEETNEMMDGSSKKIAKIITEINAIHTDRTPKRLQFTQEVAEGTLNRSAEVLEKVTPILKRVEQWDGNMKSEAYSAAAFDRTVLSAGEAVKDLSAIVPELLSKLRVVEEKKPVNNVTTSIMKIRGLIAQARSVAKKVQVSMKFDGQSAVEVHPHTNLDELKTVTSISFYIRVDPDKDPIEDRFLLYLGDKQGKRDYMGLAIKNDNLVYVYKLGEEEVEIPLSSKPVSSWPPVFNFVKVERLGRHGKVFLTVPSQDTTAEQKFIQKGEPVGTDTLFDLDPKDMVFIVGGVPPGVTVPPILSLAPFVGCIELETLNNDVISLYNFKQLHKMDVKASAPCPRHKLAFSQSRVTSYLFDGTGYALVNNIERRGKIGVVTRFDIEVRTVANNGILFLMVNETNFFVLELKNGFLRLMYDFGFANGPVIIETNLAKLQINDARYHEVSVIYHHSKKIILLVDRSHVKSFESEKKPLPFSDIYIGGAPSRILQSRPELTSLIGLKGCVKGFQFQKEDFNLLEEQGTIGISSGCPEESFMSHKAYFTGNGYLSSSDKISPFQSFEGGLSFKTLQPSGLLFYHRDGSDEFSLSLENGAVVLQAKGTRVKSQKKHYSDGRSHFLVASVTNQKYEIVIDDKDKQDKKKPSSTSQSDNTPKAFYYGGSTSSSILNLTGCISYAYISRQDRDIEPEDFQRYSENVQASLQDCPIERPPSALLKDSRDSSRRSAGHSRKVGRDESGLPHGLKSNPLNIPEKEEASCYLSSRPRATPHANHYGGSAHSRHEYTGIASAIKHESHFSLSLKTRSAFGLIFYLADASGENSMALFLTHARLVFTFSSGQSQVRIRSKEKYNDGQWHDIIFIRAGNMGRMLIDGLTVLEDRAPGRNTSLLVQDPLYVGGVPPHQAMKNIQRTSVSSFTGCVRSLQLNGRSLTSASHSFGVTPCYEGPSEPGTYFAEEGGYVLLDDSFHFGSSFKMAMEVRPRVASGVLLHVFMEQKEYLTVYIYQSQVVVVVNNGIAEFSTHVSPTQEICDGNWHKITVIRDGSVLQLDVDSEVNHVVGSVSDAAQSSSSPVFIGGAPDSMLSHSLLSRRGFTGCMRSVSVNETPVSFSKAVLVSGSVGVGACPSA

Target ORF information:

RefSeq Version	NM_001039065.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio laminin, alpha 4 (lama4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039065.2

ORF Insert Sequence:

ATGGCCTCTC TGCTGTCCGG ATCTGTCTGC TTTCTGTATG TCTTGTCAGC ACTCGTGCCC 
GCAGATTCAA CACAACAGTT CAACACAGTC TACAGGGTGT GTGCTCGAGG GTACTTCTTG 
AGTGATCAGG AGACATGTCT GCCCTGTAAC TGTAAAGGCC ATGCGGATAG CTGTGAGGAC 
ATCACCGGGA TCTGTATTGA TTGTCGGGAC CACACTCTCG GAGATTTCTG TGAGCTGTGT 
GAAGACAGAT TTTTGCCCGT GTTTCTTCCT GATGGCACCT TTATGTGTAG ACCGTGTGCC 
TGCCCGCTGT CACTGGAGTC TAACAACTTT GCAGCTCACT GTGATAGGAG GGGTGAGTTT 
CTGAGATGTG TCTGCCAGGA CGGGTATGCT GGGCACTACT GCGAGAGATG TGCTCCGGGT 
TATTATGGCA ACCCCATGGA GTTGGGCAAT TCTTGCAAAA AGTGTGATTG TAACGGCAAC 
TCTGACCCCA ACCTGATCTT CAACGAGTGC CATAATGTGA CAGGAAAGTG CCTGAACTGC 
TGGGGTGACA CTGCAGGGGA CAACTGTGAA AGGTGTGCAC CTGGTTTCTA TGGCGACGCC 
ATCAGTGCTA AAGACTGTCA AGAGTGCCAG TGCAATAAGT GTGGTACTGC ATCATGTGAT 
GACAGGTCAG GTGTGTGCCA CTGTAAGCCT GGGGTCACAG GTCAGCTGTG TGACAGGTGT 
GAGGACGGTC ATTCTGGATT TTGCACCTGC ATGGGTTGCC GTCGATGTGA ATGTGCTTCT 
GCTGCCCTGC GTGCCACCTG CCACCCTCTA ACACACAGCT GCCAGTGCCG CCCGGGGGCT 
GGAGGTCGAT ACTGCGAGCG CTGCCTGCCG GGATTCTGGG ATTACAGCCC ATCCGGCTGC 
AGGAAATGCG ACTGTCCAGA AGAGAACTGT GACCTGCACA CAGGAGAGTG TCTGCCTGAG 
CTCTCGCAAA TCAGCGAGTG TAATATCAGC TGTGACGCGT GTATCTGGAA TCTGATCGGA 
GACATTCGCC AGTCCAACAA AACTCTGGAC CAGGTGAGGA ACAGCGTGCT GAACATTTCC 
ACAGGAGCTG CGGCTAATGA CAGACTGAAG TACTACAACT ACACTGCGCA CCGGCTACAG 
GCTCAGTTTA GGGGCTGGAG AAAGAATTAT GCCATGATGA GTGGAGATAC AGATGTCCTG 
GAAGAAGAAA CTCAAAATCT GCTGACTGAC ATGGACCAGT TCGCAGAAGA GGAGAATGAA 
GTGCAGAATT TGGGAAAGCA AGTGGATCAA GAGACACTGA ACAGCTCCAC ACGAGCAAAG 
ACCCTCACTG CTAACCTGTC ATCGCTCAAT GTCCTCATTG AAGATATGAT CAGGGACTGG 
GAATTATACG GTGTCTATCA GGAGTTGGAT CCTCAGGTGA AGTTGCAGAA ACTGGCTGAA 
GCTGAACGGA TCTTGGGCTG GATGAGAAAA CTGAACCTGT CACCCAAAGA ACCGCTTGCT 
ACAGATGAGT CCTCCGAGGC GCATGAGTTG CTCAGACGGG TGCGCAGCTT TGACAGAAAT 
CTGATGACTA CACAGGGCCG TCTGCCACCT ATGCAGGAAG TTCTGGGTCG GTTTACTTCT 
AAACTCCTTC AAGCTCAGGA TCTCCTTAGC AAAGCTGAAG ACGCACTGAA ACAGGCCATC 
AACAAATACA AGAGCAACCA GCTCAAACTA CAGAGGAGAG AGGCACAGCA GCAACGTCTG 
AAAGACATCC AGGACGGCGT CGATCAAACA ATTGTTAATG CTGCGGCAGT TCTCTCTGAG 
GCAAATCACA CTGTTCTTGA GGTGGAAGAT GTGATCACGA ATGTGAGAGA ACAGCATGCA 
GAAATTGACG GAGCCCGTGT CTTACTGGAG AGAAGGACAG AGAAGATATC TCAGTCAGAC 
AGAGAACTAG TTCTGCGAGC CGAGGATCAT GCCGATCAAC TGCAGAGACA CGCTGAAGAG 
CCGCAGCATA ATCTGAAAGG AAGCGATGCG AACGGACATG TGCAGAAAGC CCTGAACGCC 
TTCAACGTGT ACGACAATAT TGTCAAGAGC GTAGACGATG CTAACATCAC TAGCCTGACC 
GCCCTCAACA TCTCCAGCAG GACTCACGGT GCGGTCGATA AGCTAAGCTC TCAGCTGAAC 
CTCATGCGCT CTCAGAGTGC AAAAGTGTTG AATGAAGCAT CGTCCCTACA CTTTCAGCAG 
CAAGACAGTG AATCTATCGT ACTCGACAAT AAGAAATACA TTGAAGAGAC CAATGAAATG 
ATGGACGGCT CCTCTAAAAA AATAGCTAAG ATTATCACGG AGATCAATGC AATTCACACA 
GATCGTACCC CGAAGCGTCT GCAGTTCACT CAGGAAGTGG CTGAGGGCAC TCTGAACCGC 
TCCGCTGAGG TGTTGGAGAA AGTGACTCCC ATTCTGAAGA GAGTGGAGCA GTGGGACGGC 
AACATGAAGA GTGAAGCCTA TTCTGCTGCA GCGTTCGACC GCACTGTTCT GTCTGCTGGC 
GAGGCGGTGA AGGACCTGTC AGCCATTGTG CCAGAGCTGC TCAGCAAGCT GCGTGTGGTT 
GAGGAGAAAA AACCTGTGAA TAACGTCACC ACGAGCATCA TGAAAATCAG AGGGCTCATC 
GCACAGGCCC GAAGCGTTGC AAAGAAGGTG CAAGTGTCCA TGAAGTTTGA TGGCCAGTCT 
GCTGTAGAAG TTCATCCCCA CACCAACCTG GATGAGCTGA AGACGGTGAC GTCCATCAGT 
TTCTACATCC GAGTAGACCC AGATAAAGAC CCCATAGAGG ATCGCTTCTT GCTTTACCTC 
GGAGACAAAC AAGGAAAGAG GGACTACATG GGACTCGCCA TAAAGAACGA TAATCTGGTG 
TACGTCTACA AACTGGGTGA AGAGGAAGTG GAGATTCCAC TCAGCTCCAA GCCAGTCAGC 
TCCTGGCCTC CTGTCTTCAA CTTTGTTAAA GTGGAGAGGC TGGGACGCCA TGGCAAAGTG 
TTCCTGACGG TTCCCAGTCA AGACACCACA GCCGAGCAGA AGTTCATCCA GAAAGGAGAG 
CCAGTCGGGA CAGACACGCT GTTTGACCTG GACCCTAAAG ACATGGTGTT CATAGTTGGA 
GGAGTGCCTC CTGGTGTCAC GGTTCCTCCG ATTTTGAGCC TGGCTCCTTT CGTTGGCTGC 
ATTGAGTTAG AGACCCTGAA TAATGATGTC ATCAGTTTGT ACAACTTTAA GCAACTTCAC 
AAAATGGATG TTAAAGCATC GGCGCCTTGT CCCAGGCACA AGCTGGCTTT CTCTCAGAGT 
CGTGTCACCA GTTATTTGTT TGACGGCACC GGCTACGCTT TAGTCAACAA CATTGAGAGG 
CGAGGAAAGA TTGGCGTCGT CACTCGATTT GATATTGAAG TTCGAACTGT TGCTAACAAC 
GGCATCCTCT TCCTCATGGT GAATGAGACC AATTTCTTTG TGCTGGAGCT GAAAAATGGC 
TTTCTCCGTC TGATGTACGA CTTTGGTTTC GCTAACGGTC CTGTCATCAT AGAGACTAAC 
TTAGCCAAAC TGCAGATCAA TGACGCTCGC TATCACGAGG TGTCTGTGAT CTATCATCAT 
TCAAAGAAGA TCATTTTGTT GGTGGACAGA AGCCATGTCA AGTCCTTTGA AAGTGAAAAG 
AAACCCCTAC CCTTTTCTGA TATCTATATA GGAGGAGCTC CTTCGAGAAT TCTGCAGTCT 
AGGCCAGAGC TGACATCCTT GATTGGATTG AAGGGTTGTG TAAAAGGCTT CCAGTTCCAG 
AAAGAGGATT TCAACCTCTT GGAGGAGCAA GGAACCATTG GTATCAGCAG CGGCTGTCCT 
GAGGAATCGT TTATGTCCCA TAAGGCGTAT TTCACTGGCA ATGGTTACCT GAGCTCTTCA 
GATAAAATCT CTCCATTCCA ATCATTTGAA GGAGGACTGA GCTTCAAAAC ACTACAGCCC 
AGCGGGCTCC TGTTCTACCA CAGAGATGGG TCGGATGAGT TTTCTCTTTC TCTGGAGAAC 
GGTGCTGTGG TACTCCAGGC CAAAGGAACT AGAGTGAAAT CCCAGAAGAA GCATTACAGT 
GATGGCCGCT CTCATTTCTT AGTCGCCTCT GTCACCAATC AGAAATATGA AATTGTCATT 
GATGACAAAG ACAAGCAGGA TAAGAAGAAG CCGTCTTCCA CGTCTCAGTC AGACAACACT 
CCAAAGGCCT TCTACTATGG AGGCTCTACA TCCAGTTCCA TACTCAATTT AACTGGGTGC 
ATCAGTTATG CCTACATCAG CAGGCAGGAC AGAGATATAG AGCCAGAAGA TTTCCAGCGC 
TACAGTGAGA ATGTGCAGGC TTCACTACAG GACTGCCCGA TTGAAAGACC CCCGTCTGCT 
CTGCTCAAAG ACAGCAGAGA CTCCTCTAGA AGAAGTGCAG GGCACTCCCG CAAGGTGGGC 
AGGGATGAGT CCGGCCTGCC ACATGGCCTG AAGAGCAATC CTCTGAACAT TCCGGAGAAA 
GAGGAGGCGT CCTGTTACCT GTCGTCCCGC CCACGAGCAA CCCCCCATGC AAATCACTAT 
GGTGGATCTG CCCACAGCAG ACATGAGTAC ACGGGCATCG CCAGTGCCAT CAAACATGAA 
TCTCACTTCT CTCTGTCTCT GAAAACCCGT TCAGCGTTTG GTCTGATCTT CTACCTCGCC 
GACGCGTCTG GGGAGAACTC AATGGCTCTG TTCCTTACTC ACGCAAGACT GGTATTCACG 
TTCAGCTCGG GCCAAAGCCA AGTCAGAATC CGGTCCAAGG AGAAGTACAA TGATGGACAG 
TGGCATGATA TTATCTTTAT CAGAGCTGGA AATATGGGCA GAATGCTAAT CGATGGTCTT 
ACGGTATTAG AGGACAGAGC TCCAGGCAGA AACACCTCGC TGCTTGTCCA GGACCCTCTC 
TATGTTGGCG GAGTCCCACC TCATCAGGCC ATGAAAAACA TTCAGAGGAC ATCTGTGTCG 
AGTTTCACTG GCTGTGTGCG GAGTCTCCAG CTGAATGGCC GCTCGCTCAC CTCTGCCTCC 
CACAGTTTTG GGGTCACCCC CTGCTACGAG GGCCCCTCGG AACCGGGGAC GTACTTCGCT 
GAGGAGGGAG GATATGTGCT GCTGGATGAC TCCTTTCATT TCGGCTCAAG CTTTAAGATG 
GCGATGGAGG TTCGCCCCCG TGTGGCTTCA GGGGTGCTAC TGCATGTCTT CATGGAGCAG 
AAGGAATATC TCACCGTGTA CATCTACCAG AGCCAGGTGG TGGTTGTGGT GAATAACGGT 
ATCGCGGAGT TCTCCACCCA CGTCTCTCCC ACACAAGAAA TCTGTGATGG AAACTGGCAC 
AAGATCACAG TGATCAGAGA TGGTAGCGTG CTTCAGCTGG ATGTGGACTC AGAGGTGAAT 
CACGTTGTGG GGTCTGTCAG TGACGCAGCC CAGAGCAGCA GTTCGCCAGT GTTTATCGGT 
GGAGCTCCAG ACTCCATGCT GTCCCACAGT CTGCTCAGCA GGAGAGGCTT CACGGGCTGC 
ATGAGGAGCG TGTCTGTAAA CGAGACTCCT GTGAGCTTCA GCAAAGCTGT GCTGGTCAGT 
GGATCAGTCG GGGTGGGCGC CTGTCCTTCT GCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cardiac transcriptome and dilated cardiomyopathy genes in zebrafish.
Circulation. Cardiovascular genetics8(2)261-9(2015 Apr)
Shih YH,Zhang Y,Ding Y,Ross CA,Li H,Olson TM,Xu X

Epistatic dissection of laminin-receptor interactions in dystrophic zebrafish muscle.
Human molecular genetics21(21)4718-31(2012 Nov)
Sztal TE,Sonntag C,Hall TE,Currie PD

Global identification of SMAD2 target genes reveals a role for multiple co-regulatory factors in zebrafish early gastrulas.
The Journal of biological chemistry286(32)28520-32(2011 Aug)
Liu Z,Lin X,Cai Z,Zhang Z,Han C,Jia S,Meng A,Wang Q

Characterization of the laminin gene family and evolution in zebrafish.
Developmental dynamics : an official publication of the American Association of Anatomists240(2)422-31(2011 Feb)
Sztal T,Berger S,Currie PD,Hall TE

Concomitant duplications of opioid peptide and receptor genes before the origin of jawed vertebrates.
PloS one5(5)e10512(2010 May)
Sundstr?m G,Dreborg S,Larhammar D

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lama4 cDNA ORF clone, Danio rerio(zebrafish)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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