smc5 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	smc5
Entrez Gene ID	566749
Full Name	structural maintenance of chromosomes 5
Synonyms	SMC5L1,pufa,pum3,wu:fb70d10,wu:fi47h05,zgc:152845,zgc:66377
General protein information	Preferred Names structural maintenance of chromosomes 5 Names structural maintenance of chromosomes protein 5
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001193541.1	NP_001180470.1	structural maintenance of chromosomes protein 5
XM_005155575.3	XP_005155632.1	structural maintenance of chromosomes protein 5 isoform X1
XM_005155575.4	XP_005155632.1	structural maintenance of chromosomes protein 5 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Smc5	NP_001099827.1
Danio rerio (zebrafish)	smc5	NP_001180470.1
Drosophila melanogaster (fruit fly)	Smc5	NP_996141.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011623	XP_320881.2
Homo sapiens (human)	SMC5	NP_055925.2
Bos taurus (cattle)	SMC5	XP_002689686.1
Arabidopsis thaliana (thale cress)	SMC5	NP_197096.1
Xenopus tropicalis (tropical clawed frog)	smc5	XP_004910829.1
Pan troglodytes (chimpanzee)	SMC5	XP_520066.3
Mus musculus (house mouse)	Smc5	NP_001239613.1
Kluyveromyces lactis	KLLA0F07997g	XP_455442.1
	SMC5	XP_001090492.2
Canis lupus familiaris (dog)	SMC5	XP_533529.3
Saccharomyces cerevisiae (baker's yeast)	SMC5	NP_014608.1
Schizosaccharomyces pombe (fission yeast)	smc5	NP_594907.2
Neurospora crassa	NCU09065	XP_963793.1
Gallus gallus (chicken)	SMC5	NP_001034424.1
Eremothecium gossypii	AGOS_AEL337C	NP_984523.2
Magnaporthe oryzae (rice blast fungus)	MGG_12590	XP_003720930.1
Oryza sativa (rice)	Os05g0596600	NP_001056521.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

hPuf-A/KIAA0020 modulates PARP-1 cleavage upon genotoxic stress.
Chang HY, Fan CC, Chu PC, Hong BE, Lee HJ, Chang MS
Cancer research71(3)1126-34(2011 Feb)

A novel puf-A gene predicted from evolutionary analysis is involved in the development of eyes and primordial germ-cells.
Kuo MW, Wang SH, Chang JC, Chang CH, Huang LJ, Lin HH, Yu AL, Li WH, Yu J
PloS one4(3)e4980(2009)

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Knockdown of puf-A in zebrafish embryos resulted in microphthalmia, a small head, and abnormal primordial germ-cell (PGC) migration
Title: A novel puf-A gene predicted from evolutionary analysis is involved in the development of eyes and primordial germ-cells.

The following smc5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the smc5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa15902	XM_005155575.4 Latest version!	Danio rerio structural maintenance of chromosomes 5 (smc5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa32991	NM_001193541.1 Latest version!	Danio rerio structural maintenance of chromosomes 5 (smc5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa15902	XM_005155575.3	Danio rerio structural maintenance of chromosomes 5 (smc5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

smc5 ( XM_005155575.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155632.1 Danio rerio structural maintenance of chromosomes 5 (smc5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15902
Clone ID Related Accession (Same CDS sequence)	XM_005155575.3 , XM_005155575.4
Accession Version	XM_005155575.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	structural maintenance of chromosomes protein 5 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005155575.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGC CGCATAAGAG AAAGCGAAAA AGCCACGAAT TGTCCAACTC ACAGCCCTCC 
GATCGTGAAC CTGCAACGTC CACCAGTGCA AATGCCCGAG AAGGAGATTT CATGGAGGGC 
GCAATCGTCC GCATCACCAT GCATAACTTC CTCACGTACG ATCACTCTGA AGTTTTCCCC 
GGACCCAAAC TGAACATGAT CGTTGGAGCC AACGGCACAG GGAAGTCCAG TATCGTGTGT 
GCCATCTGTC TGGGCCTGGC TGGAAAAACA TCTGTCCTGG GCCGAGGAGA CAAGGTTGGT 
CTGTATGTGA AGAGAGGGTG TCAAAGAGGC TCAGTTGAAA TAGAGCTGTA CAGGACACGT 
GGAAACCTGA TAGTCACCAG AGAGATCCAG GTTGAGAACA ATCAGTCGAC ATGGATGCTT 
AATAAAAAAC ACGCCAGTCA GAAGGCAGTG GAGGAGGCCG TAAGAGAGCT GCACATTCAG 
GTTGGGAACT TGTGTCAGTT CCTACCACAG GAAAAAGTCG GTGAGTTTGC TAAAATGAGT 
AATAGTGAAC TTCTTGAAGC CACTGAGAAA TCTGTCGGCC CACCAGAAAT GTACGAATTT 
CACTGTGAGC TCAAGACATT TCGCACCAAA GAGAGAGATT TAGAGGTGAT CAAAGACCTT 
CAGTTGAGAA CGATTTACAC AGCGGAGGAG AGGTATAATG TGAAGAAGTC TGCTTACTCC 
AATAATGTGG TCTCCAGTAA CTCGGCTCTG CGACCACCAC AGTTCTTGAC TACCACCATA 
GATGTAGAGG AGAGGCGACA GCTGGAGGAG CAGCTGAGGG CAGCAGAAAG GCAGAAGCAG 
AGCATTGATC AGCGGATGGC AGCCATCCGG GAGCAGCAAG CTAACCTGGA CCGTCGTGAT 
AATGAGCTGC GTGCTAATAA GAAGAAACTG TCTGATCTGA AAAGCAAGAA GAGACAACTG 
GAGCAGAAAA TCAGCACAAA ACAGGACAGC TTGAGGCAGA TGGAGCAGAA TGAGATCAAC 
TTGGTGGCAA TCGAAGAGGA AGCGAATGCC AAGATTGCTG CTGTCAACAA CAAGAAAGTG 
ACCATAATGG GGGAGTATTT GAGTCACCTG CAGTCAAAGG CCAGGCTGAA TATGGAGAAG 
GTGTATTTGG CTCTCCAGAG CGCAGGTCTC AGTGCTGAGA AAACCAAACT GGAGACGGAC 
GTTCGAGAAA GTTCAGCTGA ACTCAAGAGA GCAGAGGTAG ACTATACCAA GCTGGACAAG 
ATAAAGACCA ATCTGTTGAT GACTTGTAAG ACCCTGATGA AACGGGCCAG TGAGATCTGT 
AACATGACTC CAGGAGAGAC GGCCGTCCCG GAAGAACTAC ATGCAGCTTT CAGCTTGTTG 
CCTGAGACTC TGGATGAGAT CGATGCCATG CTGAATGAGG AAAGAACCAG AGCTGAGTGT 
TTCACAGGCC TCAGTGATGC TGTGGTGGAT GAATATAACA GGCGTGAGCA GGAGATTAAG 
AACTTGGAGA AGGAGCTGGA CGATAAGACT AATGAACTGA CAACCTATAG AAGAAACATT 
GCAGAGGCTA AAGAGCGCTG GCTGAATCCG CTGAAGAAGC TGGTGGAGCT GATCAATGTG 
CGCTTCAGTG ATTTCTTTCA GTCTATGCAG TGTGCAGGAG AGGTGGATCT GCACTCAGAG 
AATGAGGAGG AGTACGATAA ATATGGCATC CGCATCCAGG TGCAGTTCCG CAGAAACACA 
CGGATGCACG AGCTCACGCC ACACCATCAG AGTGGAGGAG AGCGCAGTGT CACCACCATG 
CTTTACCTCA TGTCCCTGCA GGAGCTCAAC CGCTGCCCCT TCAGAGTGGT GGACGAGATC 
AATCAGGGAA TGGATCCTGT CAACGAGAGA CGCGTGTTTG ACATTGTTGT GAGGGCGGCC 
TGTGGTGTGA ACACGTCTCA GTATTTCTTC ATCACACCTA AGCTTCTCCA GAACCTGCAG 
TACGCCGAGC AGATGACCAT CCTCTGTGTG CACAACGGCC CTCAAATGCT GCCGCCAAAC 
AAGTGGAATG AAAAGGCTTT CATCCAGCGA GCCAGACGCA GAAACCGGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155632.1
CDS	86..2179
Translation	MEQPHKRKRKSHELSNSQPSDREPATSTSANAREGDFMEGAIVRITMHNFLTYDHSEVFPGPKLNMIVGANGTGKSSIVCAICLGLAGKTSVLGRGDKVGLYVKRGCQRGSVEIELYRTRGNLIVTREIQVENNQSTWMLNKKHASQKAVEEAVRELHIQVGNLCQFLPQEKVGEFAKMSNSELLEATEKSVGPPEMYEFHCELKTFRTKERDLEVIKDLQLRTIYTAEERYNVKKSAYSNNVVSSNSALRPPQFLTTTIDVEERRQLEEQLRAAERQKQSIDQRMAAIREQQANLDRRDNELRANKKKLSDLKSKKRQLEQKISTKQDSLRQMEQNEINLVAIEEEANAKIAAVNNKKVTIMGEYLSHLQSKARLNMEKVYLALQSAGLSAEKTKLETDVRESSAELKRAEVDYTKLDKIKTNLLMTCKTLMKRASEICNMTPGETAVPEELHAAFSLLPETLDEIDAMLNEERTRAECFTGLSDAVVDEYNRREQEIKNLEKELDDKTNELTTYRRNIAEAKERWLNPLKKLVELINVRFSDFFQSMQCAGEVDLHSENEEEYDKYGIRIQVQFRRNTRMHELTPHHQSGGERSVTTMLYLMSLQELNRCPFRVVDEINQGMDPVNERRVFDIVVRAACGVNTSQYFFITPKLLQNLQYAEQMTILCVHNGPQMLPPNKWNEKAFIQRARRRNRT

Target ORF information:

RefSeq Version	XM_005155575.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio structural maintenance of chromosomes 5 (smc5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155575.3

ORF Insert Sequence:

ATGGAGCAGC CGCATAAGAG AAAGCGAAAA AGCCACGAAT TGTCCAACTC ACAGCCCTCC 
GATCGTGAAC CTGCAACGTC CACCAGTGCA AATGCCCGAG AAGGAGATTT CATGGAGGGC 
GCAATCGTCC GCATCACCAT GCATAACTTC CTCACGTACG ATCACTCTGA AGTTTTCCCC 
GGACCCAAAC TGAACATGAT CGTTGGAGCC AACGGCACAG GGAAGTCCAG TATCGTGTGT 
GCCATCTGTC TGGGCCTGGC TGGAAAAACA TCTGTCCTGG GCCGAGGAGA CAAGGTTGGT 
CTGTATGTGA AGAGAGGGTG TCAAAGAGGC TCAGTTGAAA TAGAGCTGTA CAGGACACGT 
GGAAACCTGA TAGTCACCAG AGAGATCCAG GTTGAGAACA ATCAGTCGAC ATGGATGCTT 
AATAAAAAAC ACGCCAGTCA GAAGGCAGTG GAGGAGGCCG TAAGAGAGCT GCACATTCAG 
GTTGGGAACT TGTGTCAGTT CCTACCACAG GAAAAAGTCG GTGAGTTTGC TAAAATGAGT 
AATAGTGAAC TTCTTGAAGC CACTGAGAAA TCTGTCGGCC CACCAGAAAT GTACGAATTT 
CACTGTGAGC TCAAGACATT TCGCACCAAA GAGAGAGATT TAGAGGTGAT CAAAGACCTT 
CAGTTGAGAA CGATTTACAC AGCGGAGGAG AGGTATAATG TGAAGAAGTC TGCTTACTCC 
AATAATGTGG TCTCCAGTAA CTCGGCTCTG CGACCACCAC AGTTCTTGAC TACCACCATA 
GATGTAGAGG AGAGGCGACA GCTGGAGGAG CAGCTGAGGG CAGCAGAAAG GCAGAAGCAG 
AGCATTGATC AGCGGATGGC AGCCATCCGG GAGCAGCAAG CTAACCTGGA CCGTCGTGAT 
AATGAGCTGC GTGCTAATAA GAAGAAACTG TCTGATCTGA AAAGCAAGAA GAGACAACTG 
GAGCAGAAAA TCAGCACAAA ACAGGACAGC TTGAGGCAGA TGGAGCAGAA TGAGATCAAC 
TTGGTGGCAA TCGAAGAGGA AGCGAATGCC AAGATTGCTG CTGTCAACAA CAAGAAAGTG 
ACCATAATGG GGGAGTATTT GAGTCACCTG CAGTCAAAGG CCAGGCTGAA TATGGAGAAG 
GTGTATTTGG CTCTCCAGAG CGCAGGTCTC AGTGCTGAGA AAACCAAACT GGAGACGGAC 
GTTCGAGAAA GTTCAGCTGA ACTCAAGAGA GCAGAGGTAG ACTATACCAA GCTGGACAAG 
ATAAAGACCA ATCTGTTGAT GACTTGTAAG ACCCTGATGA AACGGGCCAG TGAGATCTGT 
AACATGACTC CAGGAGAGAC GGCCGTCCCG GAAGAACTAC ATGCAGCTTT CAGCTTGTTG 
CCTGAGACTC TGGATGAGAT CGATGCCATG CTGAATGAGG AAAGAACCAG AGCTGAGTGT 
TTCACAGGCC TCAGTGATGC TGTGGTGGAT GAATATAACA GGCGTGAGCA GGAGATTAAG 
AACTTGGAGA AGGAGCTGGA CGATAAGACT AATGAACTGA CAACCTATAG AAGAAACATT 
GCAGAGGCTA AAGAGCGCTG GCTGAATCCG CTGAAGAAGC TGGTGGAGCT GATCAATGTG 
CGCTTCAGTG ATTTCTTTCA GTCTATGCAG TGTGCAGGAG AGGTGGATCT GCACTCAGAG 
AATGAGGAGG AGTACGATAA ATATGGCATC CGCATCCAGG TGCAGTTCCG CAGAAACACA 
CGGATGCACG AGCTCACGCC ACACCATCAG AGTGGAGGAG AGCGCAGTGT CACCACCATG 
CTTTACCTCA TGTCCCTGCA GGAGCTCAAC CGCTGCCCCT TCAGAGTGGT GGACGAGATC 
AATCAGGGAA TGGATCCTGT CAACGAGAGA CGCGTGTTTG ACATTGTTGT GAGGGCGGCC 
TGTGGTGTGA ACACGTCTCA GTATTTCTTC ATCACACCTA AGCTTCTCCA GAACCTGCAG 
TACGCCGAGC AGATGACCAT CCTCTGTGTG CACAACGGCC CTCAAATGCT GCCGCCAAAC 
AAGTGGAATG AAAAGGCTTT CATCCAGCGA GCCAGACGCA GAAACCGGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smc5 ( XM_005155575.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005155632.1 Danio rerio structural maintenance of chromosomes 5 (smc5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15902
Clone ID Related Accession (Same CDS sequence)	XM_005155575.3 , XM_005155575.4
Accession Version	XM_005155575.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2094bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	structural maintenance of chromosomes protein 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005155575.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAGC CGCATAAGAG AAAGCGAAAA AGCCACGAAT TGTCCAACTC ACAGCCCTCC 
GATCGTGAAC CTGCAACGTC CACCAGTGCA AATGCCCGAG AAGGAGATTT CATGGAGGGC 
GCAATCGTCC GCATCACCAT GCATAACTTC CTCACGTACG ATCACTCTGA AGTTTTCCCC 
GGACCCAAAC TGAACATGAT CGTTGGAGCC AACGGCACAG GGAAGTCCAG TATCGTGTGT 
GCCATCTGTC TGGGCCTGGC TGGAAAAACA TCTGTCCTGG GCCGAGGAGA CAAGGTTGGT 
CTGTATGTGA AGAGAGGGTG TCAAAGAGGC TCAGTTGAAA TAGAGCTGTA CAGGACACGT 
GGAAACCTGA TAGTCACCAG AGAGATCCAG GTTGAGAACA ATCAGTCGAC ATGGATGCTT 
AATAAAAAAC ACGCCAGTCA GAAGGCAGTG GAGGAGGCCG TAAGAGAGCT GCACATTCAG 
GTTGGGAACT TGTGTCAGTT CCTACCACAG GAAAAAGTCG GTGAGTTTGC TAAAATGAGT 
AATAGTGAAC TTCTTGAAGC CACTGAGAAA TCTGTCGGCC CACCAGAAAT GTACGAATTT 
CACTGTGAGC TCAAGACATT TCGCACCAAA GAGAGAGATT TAGAGGTGAT CAAAGACCTT 
CAGTTGAGAA CGATTTACAC AGCGGAGGAG AGGTATAATG TGAAGAAGTC TGCTTACTCC 
AATAATGTGG TCTCCAGTAA CTCGGCTCTG CGACCACCAC AGTTCTTGAC TACCACCATA 
GATGTAGAGG AGAGGCGACA GCTGGAGGAG CAGCTGAGGG CAGCAGAAAG GCAGAAGCAG 
AGCATTGATC AGCGGATGGC AGCCATCCGG GAGCAGCAAG CTAACCTGGA CCGTCGTGAT 
AATGAGCTGC GTGCTAATAA GAAGAAACTG TCTGATCTGA AAAGCAAGAA GAGACAACTG 
GAGCAGAAAA TCAGCACAAA ACAGGACAGC TTGAGGCAGA TGGAGCAGAA TGAGATCAAC 
TTGGTGGCAA TCGAAGAGGA AGCGAATGCC AAGATTGCTG CTGTCAACAA CAAGAAAGTG 
ACCATAATGG GGGAGTATTT GAGTCACCTG CAGTCAAAGG CCAGGCTGAA TATGGAGAAG 
GTGTATTTGG CTCTCCAGAG CGCAGGTCTC AGTGCTGAGA AAACCAAACT GGAGACGGAC 
GTTCGAGAAA GTTCAGCTGA ACTCAAGAGA GCAGAGGTAG ACTATACCAA GCTGGACAAG 
ATAAAGACCA ATCTGTTGAT GACTTGTAAG ACCCTGATGA AACGGGCCAG TGAGATCTGT 
AACATGACTC CAGGAGAGAC GGCCGTCCCG GAAGAACTAC ATGCAGCTTT CAGCTTGTTG 
CCTGAGACTC TGGATGAGAT CGATGCCATG CTGAATGAGG AAAGAACCAG AGCTGAGTGT 
TTCACAGGCC TCAGTGATGC TGTGGTGGAT GAATATAACA GGCGTGAGCA GGAGATTAAG 
AACTTGGAGA AGGAGCTGGA CGATAAGACT AATGAACTGA CAACCTATAG AAGAAACATT 
GCAGAGGCTA AAGAGCGCTG GCTGAATCCG CTGAAGAAGC TGGTGGAGCT GATCAATGTG 
CGCTTCAGTG ATTTCTTTCA GTCTATGCAG TGTGCAGGAG AGGTGGATCT GCACTCAGAG 
AATGAGGAGG AGTACGATAA ATATGGCATC CGCATCCAGG TGCAGTTCCG CAGAAACACA 
CGGATGCACG AGCTCACGCC ACACCATCAG AGTGGAGGAG AGCGCAGTGT CACCACCATG 
CTTTACCTCA TGTCCCTGCA GGAGCTCAAC CGCTGCCCCT TCAGAGTGGT GGACGAGATC 
AATCAGGGAA TGGATCCTGT CAACGAGAGA CGCGTGTTTG ACATTGTTGT GAGGGCGGCC 
TGTGGTGTGA ACACGTCTCA GTATTTCTTC ATCACACCTA AGCTTCTCCA GAACCTGCAG 
TACGCCGAGC AGATGACCAT CCTCTGTGTG CACAACGGCC CTCAAATGCT GCCGCCAAAC 
AAGTGGAATG AAAAGGCTTT CATCCAGCGA GCCAGACGCA GAAACCGGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005155632.1
CDS	97..2190
Translation	MEQPHKRKRKSHELSNSQPSDREPATSTSANAREGDFMEGAIVRITMHNFLTYDHSEVFPGPKLNMIVGANGTGKSSIVCAICLGLAGKTSVLGRGDKVGLYVKRGCQRGSVEIELYRTRGNLIVTREIQVENNQSTWMLNKKHASQKAVEEAVRELHIQVGNLCQFLPQEKVGEFAKMSNSELLEATEKSVGPPEMYEFHCELKTFRTKERDLEVIKDLQLRTIYTAEERYNVKKSAYSNNVVSSNSALRPPQFLTTTIDVEERRQLEEQLRAAERQKQSIDQRMAAIREQQANLDRRDNELRANKKKLSDLKSKKRQLEQKISTKQDSLRQMEQNEINLVAIEEEANAKIAAVNNKKVTIMGEYLSHLQSKARLNMEKVYLALQSAGLSAEKTKLETDVRESSAELKRAEVDYTKLDKIKTNLLMTCKTLMKRASEICNMTPGETAVPEELHAAFSLLPETLDEIDAMLNEERTRAECFTGLSDAVVDEYNRREQEIKNLEKELDDKTNELTTYRRNIAEAKERWLNPLKKLVELINVRFSDFFQSMQCAGEVDLHSENEEEYDKYGIRIQVQFRRNTRMHELTPHHQSGGERSVTTMLYLMSLQELNRCPFRVVDEINQGMDPVNERRVFDIVVRAACGVNTSQYFFITPKLLQNLQYAEQMTILCVHNGPQMLPPNKWNEKAFIQRARRRNRT

Target ORF information:

RefSeq Version	XM_005155575.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio structural maintenance of chromosomes 5 (smc5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005155575.4

ORF Insert Sequence:

ATGGAGCAGC CGCATAAGAG AAAGCGAAAA AGCCACGAAT TGTCCAACTC ACAGCCCTCC 
GATCGTGAAC CTGCAACGTC CACCAGTGCA AATGCCCGAG AAGGAGATTT CATGGAGGGC 
GCAATCGTCC GCATCACCAT GCATAACTTC CTCACGTACG ATCACTCTGA AGTTTTCCCC 
GGACCCAAAC TGAACATGAT CGTTGGAGCC AACGGCACAG GGAAGTCCAG TATCGTGTGT 
GCCATCTGTC TGGGCCTGGC TGGAAAAACA TCTGTCCTGG GCCGAGGAGA CAAGGTTGGT 
CTGTATGTGA AGAGAGGGTG TCAAAGAGGC TCAGTTGAAA TAGAGCTGTA CAGGACACGT 
GGAAACCTGA TAGTCACCAG AGAGATCCAG GTTGAGAACA ATCAGTCGAC ATGGATGCTT 
AATAAAAAAC ACGCCAGTCA GAAGGCAGTG GAGGAGGCCG TAAGAGAGCT GCACATTCAG 
GTTGGGAACT TGTGTCAGTT CCTACCACAG GAAAAAGTCG GTGAGTTTGC TAAAATGAGT 
AATAGTGAAC TTCTTGAAGC CACTGAGAAA TCTGTCGGCC CACCAGAAAT GTACGAATTT 
CACTGTGAGC TCAAGACATT TCGCACCAAA GAGAGAGATT TAGAGGTGAT CAAAGACCTT 
CAGTTGAGAA CGATTTACAC AGCGGAGGAG AGGTATAATG TGAAGAAGTC TGCTTACTCC 
AATAATGTGG TCTCCAGTAA CTCGGCTCTG CGACCACCAC AGTTCTTGAC TACCACCATA 
GATGTAGAGG AGAGGCGACA GCTGGAGGAG CAGCTGAGGG CAGCAGAAAG GCAGAAGCAG 
AGCATTGATC AGCGGATGGC AGCCATCCGG GAGCAGCAAG CTAACCTGGA CCGTCGTGAT 
AATGAGCTGC GTGCTAATAA GAAGAAACTG TCTGATCTGA AAAGCAAGAA GAGACAACTG 
GAGCAGAAAA TCAGCACAAA ACAGGACAGC TTGAGGCAGA TGGAGCAGAA TGAGATCAAC 
TTGGTGGCAA TCGAAGAGGA AGCGAATGCC AAGATTGCTG CTGTCAACAA CAAGAAAGTG 
ACCATAATGG GGGAGTATTT GAGTCACCTG CAGTCAAAGG CCAGGCTGAA TATGGAGAAG 
GTGTATTTGG CTCTCCAGAG CGCAGGTCTC AGTGCTGAGA AAACCAAACT GGAGACGGAC 
GTTCGAGAAA GTTCAGCTGA ACTCAAGAGA GCAGAGGTAG ACTATACCAA GCTGGACAAG 
ATAAAGACCA ATCTGTTGAT GACTTGTAAG ACCCTGATGA AACGGGCCAG TGAGATCTGT 
AACATGACTC CAGGAGAGAC GGCCGTCCCG GAAGAACTAC ATGCAGCTTT CAGCTTGTTG 
CCTGAGACTC TGGATGAGAT CGATGCCATG CTGAATGAGG AAAGAACCAG AGCTGAGTGT 
TTCACAGGCC TCAGTGATGC TGTGGTGGAT GAATATAACA GGCGTGAGCA GGAGATTAAG 
AACTTGGAGA AGGAGCTGGA CGATAAGACT AATGAACTGA CAACCTATAG AAGAAACATT 
GCAGAGGCTA AAGAGCGCTG GCTGAATCCG CTGAAGAAGC TGGTGGAGCT GATCAATGTG 
CGCTTCAGTG ATTTCTTTCA GTCTATGCAG TGTGCAGGAG AGGTGGATCT GCACTCAGAG 
AATGAGGAGG AGTACGATAA ATATGGCATC CGCATCCAGG TGCAGTTCCG CAGAAACACA 
CGGATGCACG AGCTCACGCC ACACCATCAG AGTGGAGGAG AGCGCAGTGT CACCACCATG 
CTTTACCTCA TGTCCCTGCA GGAGCTCAAC CGCTGCCCCT TCAGAGTGGT GGACGAGATC 
AATCAGGGAA TGGATCCTGT CAACGAGAGA CGCGTGTTTG ACATTGTTGT GAGGGCGGCC 
TGTGGTGTGA ACACGTCTCA GTATTTCTTC ATCACACCTA AGCTTCTCCA GAACCTGCAG 
TACGCCGAGC AGATGACCAT CCTCTGTGTG CACAACGGCC CTCAAATGCT GCCGCCAAAC 
AAGTGGAATG AAAAGGCTTT CATCCAGCGA GCCAGACGCA GAAACCGGAC CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

smc5 ( NM_001193541.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001180470.1 Danio rerio structural maintenance of chromosomes 5 (smc5), mRNA.

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CloneID	ODa32991
Clone ID Related Accession (Same CDS sequence)	NM_001193541.1
Accession Version	NM_001193541.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3222bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	structural maintenance of chromosomes protein 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX640501.15. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01011378.1, GFIL01039006.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCAGC CGCATAAGAG AAAGCGAAAA AGCCACGAAT TGTCCAACTC ACAGCCCTCC 
GATCGTGAAC CTGCAACGTC CACCAGTGCA AATGCCCGAG AAGGAGATTT CATGGAGGGC 
GCAATCGTCC GCATCACCAT GCATAACTTC CTCACGTACG ATCACTCTGA AGTTTTCCCC 
GGACCCAAAC TGAACATGAT CGTTGGAGCC AACGGCACAG GGAAGTCCAG TATCGTGTGT 
GCCATCTGTC TGGGCCTGGC TGGAAAAACA TCTGTCCTGG GCCGAGGAGA CAAGGTTGGT 
CTGTATGTGA AGAGAGGGTG TCAAAGAGGC TCAGTTGAAA TAGAGCTGTA CAGGACACGT 
GGAAACCTGA TAGTCACCAG AGAGATCCAG GTTGAGAACA ATCAGTCGAC ATGGATGCTT 
AATAAAAAAC ACGCCAGTCA GAAGGCAGTG GAGGAGGCCG TAAGAGAGCT GCACATTCAG 
GTTGGGAACT TGTGTCAGTT CCTACCACAG GAAAAAGTCG GTGAGTTTGC TAAAATGAGT 
AATAGTGAAC TTCTTGAAGC CACTGAGAAA TCTGTCGGCC CACCAGAAAT GTACGAATTT 
CACTGTGAGC TCAAGACATT TCGCACCAAA GAGAGAGATT TAGAGAATGT GTGTAAGGAA 
AAAGGAAACT TCCTAGAGAA GGCCAGACAG AGGAATGAAA GAAACAAGCT GGATGTGGAG 
CGCTACTACA TGAAGAAGCG GCATTTAGAC AGGATCCAGA TGCTTGAGAA GAAGAAACCC 
TGGGTGGAGT ATGAGACGGC ACGTAAAGAG CTGGAGGGGG TGAAGAAGGA AAGAGATGAA 
ATGAAAAGGA AGCTTAGGTT CCTGAAGGAA GCTCAGGAGC CGCTGCTCAG AAAGATCCGC 
TCTGTGGAGA GTGAGCTGCA GCCCATCGAG CAGCAGATGA AGGAGATGAC CAACCGTATC 
AAAGAGGCAA CGCAGAAATG TAAGCAGAAA CACGACCAGC TTGAACTGAA GAATAAAGAG 
GTTGATGACA TCAAACAGGA CATGAGTCTA AAGCAGACGG AGGAGGCGGA CAGGCAGAAG 
CGGATTGGAC ACACGCAACT GATGATCAGA GATCTGCAAA AGGAGCTTCA GAACATGGGC 
ACCATTGAGG ACGTTACTCC TCAGATTGAG GCTATCAATG CAGAGCTGAG GAACATTCAG 
GAAGAAAGAG CCAGACTGGA GAGTGAGAGT CTGGACCTGC GCAGAGATAA AGATGAAATC 
ACTGGAGAAT TTGCGCGTTT GCAGAATCGC CTGAGGAGTC TGGATGACAT GATGAAGATT 
AAAGAGGAGA AATTGCGCAG CCGCTTTCGT GACACATACA CAGCCCTTGA GTGGCTGAGG 
AAAAATCGTG ATCGTTATGA AGGAGTTGTC CATGAGCCCA TGATGCTGGT GATAAATGTT 
CGTGATGCTC GGCATGCAAA ATACATTGAG ACTCACATTT CTGTCAATGA CTTGAGAGCT 
TTCGTCTTCC AGAGACAGGA TGATAACGAC AAATTTATGA ATGAGATGAG AGATACGCAG 
AGGCTGCGAG TTAACAGCAT CATCGCTCCT ACAGAGTCTT GCTCTAAACG ACCACCGTCA 
CGACCCATCG AGACCCTCAA GCCTTACGGC TTCATCTCGT ACTTGCGAGA GATGTTTGAT 
GCTCCTGAGG AGGTTATGAG TTATTTATGC CATCAGTACA GAGTTAACGA TGTACCAATA 
GGAACAGAGA AGACCAAGGG CATGATTGAA TCGGTGATCA AAGACCTTCA GTTGAGAACG 
ATTTACACAG CGGAGGAGAG GTATAATGTG AAGAAGTCTG CTTACTCCAA TAATGTGGTC 
TCCAGTAACT CGGCTCTGCG ACCACCACAG TTCTTGACTA CCACCATAGA TGTAGAGGAG 
AGGCGACAGC TGGAGGAGCA GCTGAGGGCA GCAGAAAGGC AGAAGCAGAG CATTGATCAG 
CGGATGGCAG CCATCCGGGA GCAGCAAGCT AACCTGGACC GTCGTGATAA TGAGCTGCGT 
GCTAATAAGA AGAAACTGTC TGATCTGAAA AGCAAGAAGA GACAACTGGA GCAGAAAATC 
AGCACAAAAC AGGACAGCTT GAGGCAGATG GAGCAGAATG AGATCAACTT GGTGGCAATC 
GAAGAGGAAG CGAATGCCAA GATTGCTGCT GTCAACAACA AGAAAGTGAC CATAATGGGG 
GAGTATTTGA GTCACCTGCA GTCAAAGGCC AGGCTGAATA TGGAGAAGGT GTATTTGGCT 
CTCCAGAGCG CAGGTCTCAG TGCTGAGAAA ACCAAACTGG AGACGGACGT TCGAGAAAGT 
TCAGCTGAAC TCAAGAGAGC AGAGGTAGAC TATACCAAGC TGGACAAGAT AAAGACCAAT 
CTGTTGATGA CTTGTAAGAC CCTGATGAAA CGGGCCAGTG AGATCTGTAA CATGACTCCA 
GGAGAGACGG CCGTCCCGGA AGAACTACAT GCAGCTTTCA GCTTGTTGCC TGAGACTCTG 
GATGAGATCG ATGCCATGCT GAATGAGGAA AGAACCAGAG CTGAGTGTTT CACAGGCCTC 
AGTGATGCTG TGGTGGATGA ATATAACAGG CGTGAGCAGG AGATTAAGAA CTTGGAGAAG 
GAGCTGGACG ATAAGACTAA TGAACTGACA ACCTATAGAA GAAACATTGC AGAGGCTAAA 
GAGCGCTGGC TGAATCCGCT GAAGAAGCTG GTGGAGCTGA TCAATGTGCG CTTCAGTGAT 
TTCTTTCAGT CTATGCAGTG TGCAGGAGAG GTGGATCTGC ACTCAGAGAA TGAGGAGGAG 
TACGATAAAT ATGGCATCCG CATCCAGGTG CAGTTCCGCA GAAACACACG GATGCACGAG 
CTCACGCCAC ACCATCAGAG TGGAGGAGAG CGCAGTGTCA CCACCATGCT TTACCTCATG 
TCCCTGCAGG AGCTCAACCG CTGCCCCTTC AGAGTGGTGG ACGAGATCAA TCAGGGAATG 
GATCCTGTCA ACGAGAGACG CGTGTTTGAC ATTGTTGTGA GGGCGGCCTG TGGTGTGAAC 
ACGTCTCAGT ATTTCTTCAT CACACCTAAG CTTCTCCAGA ACCTGCAGTA CGCCGAGCAG 
ATGACCATCC TCTGTGTGCA CAACGGCCCT CAAATGCTGC CGCCAAACAA GTGGAATGAA 
AAGGCTTTCA TCCAGCGAGC CAGACGCAGA AACCGGACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180470.1
CDS	86..3307
Translation	MEQPHKRKRKSHELSNSQPSDREPATSTSANAREGDFMEGAIVRITMHNFLTYDHSEVFPGPKLNMIVGANGTGKSSIVCAICLGLAGKTSVLGRGDKVGLYVKRGCQRGSVEIELYRTRGNLIVTREIQVENNQSTWMLNKKHASQKAVEEAVRELHIQVGNLCQFLPQEKVGEFAKMSNSELLEATEKSVGPPEMYEFHCELKTFRTKERDLENVCKEKGNFLEKARQRNERNKLDVERYYMKKRHLDRIQMLEKKKPWVEYETARKELEGVKKERDEMKRKLRFLKEAQEPLLRKIRSVESELQPIEQQMKEMTNRIKEATQKCKQKHDQLELKNKEVDDIKQDMSLKQTEEADRQKRIGHTQLMIRDLQKELQNMGTIEDVTPQIEAINAELRNIQEERARLESESLDLRRDKDEITGEFARLQNRLRSLDDMMKIKEEKLRSRFRDTYTALEWLRKNRDRYEGVVHEPMMLVINVRDARHAKYIETHISVNDLRAFVFQRQDDNDKFMNEMRDTQRLRVNSIIAPTESCSKRPPSRPIETLKPYGFISYLREMFDAPEEVMSYLCHQYRVNDVPIGTEKTKGMIESVIKDLQLRTIYTAEERYNVKKSAYSNNVVSSNSALRPPQFLTTTIDVEERRQLEEQLRAAERQKQSIDQRMAAIREQQANLDRRDNELRANKKKLSDLKSKKRQLEQKISTKQDSLRQMEQNEINLVAIEEEANAKIAAVNNKKVTIMGEYLSHLQSKARLNMEKVYLALQSAGLSAEKTKLETDVRESSAELKRAEVDYTKLDKIKTNLLMTCKTLMKRASEICNMTPGETAVPEELHAAFSLLPETLDEIDAMLNEERTRAECFTGLSDAVVDEYNRREQEIKNLEKELDDKTNELTTYRRNIAEAKERWLNPLKKLVELINVRFSDFFQSMQCAGEVDLHSENEEEYDKYGIRIQVQFRRNTRMHELTPHHQSGGERSVTTMLYLMSLQELNRCPFRVVDEINQGMDPVNERRVFDIVVRAACGVNTSQYFFITPKLLQNLQYAEQMTILCVHNGPQMLPPNKWNEKAFIQRARRRNRT

Target ORF information:

RefSeq Version	NM_001193541.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio structural maintenance of chromosomes 5 (smc5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193541.1

ORF Insert Sequence:

ATGGAGCAGC CGCATAAGAG AAAGCGAAAA AGCCACGAAT TGTCCAACTC ACAGCCCTCC 
GATCGTGAAC CTGCAACGTC CACCAGTGCA AATGCCCGAG AAGGAGATTT CATGGAGGGC 
GCAATCGTCC GCATCACCAT GCATAACTTC CTCACGTACG ATCACTCTGA AGTTTTCCCC 
GGACCCAAAC TGAACATGAT CGTTGGAGCC AACGGCACAG GGAAGTCCAG TATCGTGTGT 
GCCATCTGTC TGGGCCTGGC TGGAAAAACA TCTGTCCTGG GCCGAGGAGA CAAGGTTGGT 
CTGTATGTGA AGAGAGGGTG TCAAAGAGGC TCAGTTGAAA TAGAGCTGTA CAGGACACGT 
GGAAACCTGA TAGTCACCAG AGAGATCCAG GTTGAGAACA ATCAGTCGAC ATGGATGCTT 
AATAAAAAAC ACGCCAGTCA GAAGGCAGTG GAGGAGGCCG TAAGAGAGCT GCACATTCAG 
GTTGGGAACT TGTGTCAGTT CCTACCACAG GAAAAAGTCG GTGAGTTTGC TAAAATGAGT 
AATAGTGAAC TTCTTGAAGC CACTGAGAAA TCTGTCGGCC CACCAGAAAT GTACGAATTT 
CACTGTGAGC TCAAGACATT TCGCACCAAA GAGAGAGATT TAGAGAATGT GTGTAAGGAA 
AAAGGAAACT TCCTAGAGAA GGCCAGACAG AGGAATGAAA GAAACAAGCT GGATGTGGAG 
CGCTACTACA TGAAGAAGCG GCATTTAGAC AGGATCCAGA TGCTTGAGAA GAAGAAACCC 
TGGGTGGAGT ATGAGACGGC ACGTAAAGAG CTGGAGGGGG TGAAGAAGGA AAGAGATGAA 
ATGAAAAGGA AGCTTAGGTT CCTGAAGGAA GCTCAGGAGC CGCTGCTCAG AAAGATCCGC 
TCTGTGGAGA GTGAGCTGCA GCCCATCGAG CAGCAGATGA AGGAGATGAC CAACCGTATC 
AAAGAGGCAA CGCAGAAATG TAAGCAGAAA CACGACCAGC TTGAACTGAA GAATAAAGAG 
GTTGATGACA TCAAACAGGA CATGAGTCTA AAGCAGACGG AGGAGGCGGA CAGGCAGAAG 
CGGATTGGAC ACACGCAACT GATGATCAGA GATCTGCAAA AGGAGCTTCA GAACATGGGC 
ACCATTGAGG ACGTTACTCC TCAGATTGAG GCTATCAATG CAGAGCTGAG GAACATTCAG 
GAAGAAAGAG CCAGACTGGA GAGTGAGAGT CTGGACCTGC GCAGAGATAA AGATGAAATC 
ACTGGAGAAT TTGCGCGTTT GCAGAATCGC CTGAGGAGTC TGGATGACAT GATGAAGATT 
AAAGAGGAGA AATTGCGCAG CCGCTTTCGT GACACATACA CAGCCCTTGA GTGGCTGAGG 
AAAAATCGTG ATCGTTATGA AGGAGTTGTC CATGAGCCCA TGATGCTGGT GATAAATGTT 
CGTGATGCTC GGCATGCAAA ATACATTGAG ACTCACATTT CTGTCAATGA CTTGAGAGCT 
TTCGTCTTCC AGAGACAGGA TGATAACGAC AAATTTATGA ATGAGATGAG AGATACGCAG 
AGGCTGCGAG TTAACAGCAT CATCGCTCCT ACAGAGTCTT GCTCTAAACG ACCACCGTCA 
CGACCCATCG AGACCCTCAA GCCTTACGGC TTCATCTCGT ACTTGCGAGA GATGTTTGAT 
GCTCCTGAGG AGGTTATGAG TTATTTATGC CATCAGTACA GAGTTAACGA TGTACCAATA 
GGAACAGAGA AGACCAAGGG CATGATTGAA TCGGTGATCA AAGACCTTCA GTTGAGAACG 
ATTTACACAG CGGAGGAGAG GTATAATGTG AAGAAGTCTG CTTACTCCAA TAATGTGGTC 
TCCAGTAACT CGGCTCTGCG ACCACCACAG TTCTTGACTA CCACCATAGA TGTAGAGGAG 
AGGCGACAGC TGGAGGAGCA GCTGAGGGCA GCAGAAAGGC AGAAGCAGAG CATTGATCAG 
CGGATGGCAG CCATCCGGGA GCAGCAAGCT AACCTGGACC GTCGTGATAA TGAGCTGCGT 
GCTAATAAGA AGAAACTGTC TGATCTGAAA AGCAAGAAGA GACAACTGGA GCAGAAAATC 
AGCACAAAAC AGGACAGCTT GAGGCAGATG GAGCAGAATG AGATCAACTT GGTGGCAATC 
GAAGAGGAAG CGAATGCCAA GATTGCTGCT GTCAACAACA AGAAAGTGAC CATAATGGGG 
GAGTATTTGA GTCACCTGCA GTCAAAGGCC AGGCTGAATA TGGAGAAGGT GTATTTGGCT 
CTCCAGAGCG CAGGTCTCAG TGCTGAGAAA ACCAAACTGG AGACGGACGT TCGAGAAAGT 
TCAGCTGAAC TCAAGAGAGC AGAGGTAGAC TATACCAAGC TGGACAAGAT AAAGACCAAT 
CTGTTGATGA CTTGTAAGAC CCTGATGAAA CGGGCCAGTG AGATCTGTAA CATGACTCCA 
GGAGAGACGG CCGTCCCGGA AGAACTACAT GCAGCTTTCA GCTTGTTGCC TGAGACTCTG 
GATGAGATCG ATGCCATGCT GAATGAGGAA AGAACCAGAG CTGAGTGTTT CACAGGCCTC 
AGTGATGCTG TGGTGGATGA ATATAACAGG CGTGAGCAGG AGATTAAGAA CTTGGAGAAG 
GAGCTGGACG ATAAGACTAA TGAACTGACA ACCTATAGAA GAAACATTGC AGAGGCTAAA 
GAGCGCTGGC TGAATCCGCT GAAGAAGCTG GTGGAGCTGA TCAATGTGCG CTTCAGTGAT 
TTCTTTCAGT CTATGCAGTG TGCAGGAGAG GTGGATCTGC ACTCAGAGAA TGAGGAGGAG 
TACGATAAAT ATGGCATCCG CATCCAGGTG CAGTTCCGCA GAAACACACG GATGCACGAG 
CTCACGCCAC ACCATCAGAG TGGAGGAGAG CGCAGTGTCA CCACCATGCT TTACCTCATG 
TCCCTGCAGG AGCTCAACCG CTGCCCCTTC AGAGTGGTGG ACGAGATCAA TCAGGGAATG 
GATCCTGTCA ACGAGAGACG CGTGTTTGAC ATTGTTGTGA GGGCGGCCTG TGGTGTGAAC 
ACGTCTCAGT ATTTCTTCAT CACACCTAAG CTTCTCCAGA ACCTGCAGTA CGCCGAGCAG 
ATGACCATCC TCTGTGTGCA CAACGGCCCT CAAATGCTGC CGCCAAACAA GTGGAATGAA 
AAGGCTTTCA TCCAGCGAGC CAGACGCAGA AACCGGACCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

hPuf-A/KIAA0020 modulates PARP-1 cleavage upon genotoxic stress.
Cancer research71(3)1126-34(2011 Feb)
Chang HY,Fan CC,Chu PC,Hong BE,Lee HJ,Chang MS

A novel puf-A gene predicted from evolutionary analysis is involved in the development of eyes and primordial germ-cells.
PloS one4(3)e4980(2009)
Kuo MW,Wang SH,Chang JC,Chang CH,Huang LJ,Lin HH,Yu AL,Li WH,Yu J

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

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smc5 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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