SI:DKEY-110K5.6 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SI:DKEY-110K5.6
Entrez Gene ID	566657
Full Name	si:dkey-110k5.6
Synonyms	tbc1d8b,wu:fc28a09
General protein information	Preferred Names si:dkey-110k5.6 Names TBC1 domain family member 8B fc28a09
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009301355.2	XP_009299630.1	TBC1 domain family member 8B isoform X4
XM_005165180.4	XP_005165237.1	TBC1 domain family member 8B isoform X2
XM_009301355.3	XP_009299630.1	TBC1 domain family member 8B isoform X3
XM_009301354.2	XP_009299629.1	TBC1 domain family member 8B isoform X3
NM_001127515.1	NP_001120987.1	TBC1 domain family member 8B
XM_005165180.3	XP_005165237.1	TBC1 domain family member 8B isoform X2
XM_009301353.3	XP_009299628.1	TBC1 domain family member 8B isoform X1
XM_009301353.2	XP_009299628.1	TBC1 domain family member 8B isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TBC1D8B	XP_521207.4
Homo sapiens (human)	TBC1D8B	NP_060222.2
Rattus norvegicus (Norway rat)	Tbc1d8b	XP_003749528.1
Gallus gallus (chicken)	TBC1D8B	XP_420352.3
Danio rerio (zebrafish)	si:dkey-110k5.6	NP_001120987.1
Canis lupus familiaris (dog)	TBC1D8B	XP_549172.3
Mus musculus (house mouse)	Tbc1d8b	NP_001074968.1
	TBC1D8B	XP_002806409.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SI:DKEY-110K5.6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SI:DKEY-110K5.6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa01567	NM_001127515.1 Latest version!	Danio rerio TBC1 domain family member 8B (tbc1d8b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa10298	XM_005165180.4 Latest version!	Danio rerio si:dkey-110k5.6 (si:dkey-110k5.6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa44934	XM_009301353.3 Latest version!	Danio rerio si:dkey-110k5.6 (si:dkey-110k5.6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa44935	XM_009301354.2 Latest version!	Danio rerio si:dkey-110k5.6 (si:dkey-110k5.6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa44936	XM_009301355.3 Latest version!	Danio rerio si:dkey-110k5.6 (si:dkey-110k5.6), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa44936	XM_009301355.2	Danio rerio si:dkey-110k5.6 (si:dkey-110k5.6), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa10298	XM_005165180.3	Danio rerio si:dkey-110k5.6 (si:dkey-110k5.6), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa44934	XM_009301353.2	Danio rerio si:dkey-110k5.6 (si:dkey-110k5.6), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SI:DKEY-110K5.6 ( NM_001127515.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001120987.1 Danio rerio TBC1 domain family member 8B (tbc1d8b), mRNA.

Price & Availability↑

CloneID	ODa01567
Clone ID Related Accession (Same CDS sequence)	NM_001127515.1
Accession Version	NM_001127515.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3327bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-23
Organism	Danio rerio(zebrafish)
Product	TBC1 domain family member 8B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AL929305.4. On Jul 31, 2008 this sequence version replaced XM_689936.3. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTGGCTCA AACCCGAAGA GGTGCTTCTC AAAAACGCGC TGAAATTATG GGTGACGGAG 
AAATCCAACG ATTACTTCGT GCTGCAGCGC AGACGCGGAT ACGGAGAGGA CAGCGGGGGA 
TTGACAGGCT TGCTGGTCGG AACCTTGGAC ACAGTTCTTG ACTCCACAGC CAAAGTGGCC 
CCGTTTCGCA TTTTACACCA GACACCCGAC TCTCAAGTGT ACTGGACTAT CGCCTGTGGA 
GCATCTTTAG AGGAGATCTC GCAGCACTGG GACTGGCTGC AGCAGAACAT TGTCAGAACT 
CTCTCTGTGT TCGACTCTGG CGAAGACATC ACCAGTTTTG TTCAGGGAAA GATAAGGGGT 
CTGATAGCAG AGGAAGGCAC ATCTGCAGGT GATGAGGAGG ATCCTGAGCG CTTCCGGGAG 
GCAGTGCTTC GCTTTGAACG CCTGTTTGGT TTGCCGCAGA GAGAAAAGCT TGTGACATAT 
TTCTCATGCA GCTACTGGAG AGGACGGGTG CCGAACCAGG GCTGGATCTA CCTCAGCACC 
AACTTCCTGT GTTTCTACTC CTATATGCTT GGAAATGAAG TGAAACTGGT GTATCCGTGG 
GATGAAGTGA GCCGTCTGGA ACGCACGTCG AGTGTTTTAT TGGCAGAGAG TATCCGTGTG 
CGAGTGCGTG GAGAGGATCA TTTCTTCTCG ATGCTTCTGA GGCTTCAGCA GACATACCTG 
ATCATGCAGC AGCTGGCCGA CTACGCCATT GTGCGCTTCT TCGATAAAGA AACATTTCAT 
GCTGAACATC CACTAGCCAA CCCCCTTCAC ATCACGCAGA GGGCTTTGGA AATACATGCA 
CGGAATCAGT CTTTCCGATC GTTTTTCCGG TTGCCGCAGG AGGAGAACCT TTGTGAAGTG 
TATGAGAGTT TCCTCTGGGT GCCGTTTAGT CACGTCAACA CTCTTGGAAA AATCTGTGTC 
TCGGAAAACT ATCTGTGCTT TGCAAGCCAA GATGGGTCTC AGTGCCATCT CATAATACCT 
CTATGGGAGG TGTTCAGTGT GGAGCTGCCA GACCGCAGCA GTCGAGCTCT GACTGTGTGC 
CTTCGAGGCA AGAGAGCTTT GCGTTTCTCT GAGGTTCGGG ATTTTGAGCG ACTTGCTGCC 
ACCATCCGCA GAAAGTGTGG GACATTGGGG AGCCCTCAGC ACTGCATCAC TAATCCGGAT 
GAGGAAGGTG TGATGGTGGG ACAAAGTCAA GCTGTCAGCA CTGAAGCTCT AATGAACGTC 
TTTCATCCAC ATGATGCAGA AAACCTTGAC CCCAAAATGC TGAAAGAGCG GATGAAGGAG 
CAGTCGTGGC AGATCCACTT TGCTGAATAT GGGCGTGGAA CTGGAATGTT TTGCACCAAA 
AAAACACGTG ACCTCATTGT GCGAGGAGTT CCAGAGACTC TCAGAGGAGA ACTGTGGATG 
CTCTTCTCAG GTGCAGTACA TGACATGATC TCTCACCCTG GGTATTACGG GCGGCTGCTG 
GAAGATTGTA TGGGCAGCAG CTCACTCGCA TGTGATGAGA TTGAGAGAGA TCTGCATCGC 
TCTCTCCCAG AACACCCAGC ATTCCAGAGC GACACGGGAA TATCAGCTCT GCGCCGGGTC 
CTCACTGCCT ATGCTCACCG CAACCCCAAA ATCGGCTACT GCCAGGCGAT GAATATACTG 
ACGTCAGTGT TGCTGCTGTA TGCTAAAGAA GAGGAAGCCT TCTGGCTGCT GGTGGCCGTG 
TGTGAAAGGA TGCTTCCGGA TTACTTCAAT CGCAGAATTA TCGGTGCTCT GGTGGACCAG 
GCTGTGTTCG AGGAGCTGAT CCGTGAACAT CTCACCCAGC TGACTGAACA CATGACAGAC 
CTGTCCTTCT TCTCGTCAGT GTCTTTATCG TGGTTTCTGA CGCTCTTCAT CAGTGTACTG 
CCAATAGAGA GCGCTGTGAA CGTGGTCGAC TGTTTCTTCT ACGATGGAAT CAAAGCCATC 
CTGCAGCTGG GCCTCGCTGT CCTCGACTAC AACATGGACA ATCTGCTCTG CTGCAATGAT 
GACGCAGAAG CCGTCACTGT CCTTAACAGG TTTTTTGACA GTGTCACAAA TAAAGACAGT 
CCCTTGCCAG CAACTGTTCA GCAGGCATCT GCAACAGCCA ATGACAAATC AATACAAAAG 
GTTGACATCA GCGACCTCAT CAAAGAAGCA TATGAGAAAT ATGGAGACAT TCGAACAGAG 
GAAGTGGAAA ACATGCGAAA GAGGAACAAG CTTTATGTCA TTCAAACACT TGAAGATACA 
ACAAAACAAA ATGTATTGCG TGTGGTTGCT CAGGATGTAA AGTTCAGTGC TTCTCAGCTG 
GACGAGCTTT ACTTGCTTTT TAAGAACCAT GACCCCAGCC TGCCATATCT GGACCAGTAT 
CAGCTGGACC AGTCCCAGTT TAGCAGCCTG TTTAACCTCT TGCAGCCGTG GACCACACAC 
ACACACTCGC GCTCTCTGGC ACGGTCAGCC TTCCATCTTC TGGATGAGAA TGGAGATGGA 
CTTGTGAACT TTAAGGAATT CATTTGCGGC CTGGACATAT TATACAACAG ATCATTCACA 
GAAAAACTGA AACTTCTCTT CAAACTGCAC CTTCAACCAG ACTCTGCAGA GGACGGAGTG 
ATCAGAAAAT GTCCAGAGAG AGGCCGTGCC AAAGTAGATC TGCAGGAGTA TCTGAAACAG 
TGGCAGGAGG ACTTACAGCG GAGAGAGGAG AATATTAAAG ATCTGCCCAG AATTAATCAG 
GTGCAGTTCA TTAGTTTGAC CAAGACTCTG TACAGCGTTT TCCACGGCGA TGAGGAGGAG 
GAGAGCCTGT ACCGCGCTGT GGCACGAGTG ACCAGTCTTC TGTTGCGCAT GGAGGAAGTG 
GGACGGAAAC TACAAGACAG CAGCCCGCAG AAAACACCCC AAACTACACC CACCAGCACA 
TCTCAGCCAG AATCCTCCCC AACAAAACCC ACCAGCCCTG AATCCGAGAC CCCCGCTGAG 
AGTCGAAGCA CCCACGACCA ACCCGAGTCT CCTGTCAGTC AACACGAAAC TGCTCCATCT 
CACTCTGACA TCACCCCGAA CTCAACCTCG CACCCTTCCA CACCCACCAG CAGTCCCACT 
GAAACCAGCA GCCCAGTGCT GGACACGCCA ACAGACACCC CCAGCTCGCC CTGCACGGTC 
AGAGACGGAG ACTGGAGTTT CTCCTTCGAG CAGATTTTGG CTTCTCTCCT GAATGAACCA 
TCTGTCGTTC GCTTCTTTGA ACGGGTCGTT AACACGGACA CTTTGATCAC TCGCGCACGC 
AAAAACCAGC TCAAAGACGC ACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120987.1
CDS	6..3332
Translation	MWLKPEEVLLKNALKLWVTEKSNDYFVLQRRRGYGEDSGGLTGLLVGTLDTVLDSTAKVAPFRILHQTPDSQVYWTIACGASLEEISQHWDWLQQNIVRTLSVFDSGEDITSFVQGKIRGLIAEEGTSAGDEEDPERFREAVLRFERLFGLPQREKLVTYFSCSYWRGRVPNQGWIYLSTNFLCFYSYMLGNEVKLVYPWDEVSRLERTSSVLLAESIRVRVRGEDHFFSMLLRLQQTYLIMQQLADYAIVRFFDKETFHAEHPLANPLHITQRALEIHARNQSFRSFFRLPQEENLCEVYESFLWVPFSHVNTLGKICVSENYLCFASQDGSQCHLIIPLWEVFSVELPDRSSRALTVCLRGKRALRFSEVRDFERLAATIRRKCGTLGSPQHCITNPDEEGVMVGQSQAVSTEALMNVFHPHDAENLDPKMLKERMKEQSWQIHFAEYGRGTGMFCTKKTRDLIVRGVPETLRGELWMLFSGAVHDMISHPGYYGRLLEDCMGSSSLACDEIERDLHRSLPEHPAFQSDTGISALRRVLTAYAHRNPKIGYCQAMNILTSVLLLYAKEEEAFWLLVAVCERMLPDYFNRRIIGALVDQAVFEELIREHLTQLTEHMTDLSFFSSVSLSWFLTLFISVLPIESAVNVVDCFFYDGIKAILQLGLAVLDYNMDNLLCCNDDAEAVTVLNRFFDSVTNKDSPLPATVQQASATANDKSIQKVDISDLIKEAYEKYGDIRTEEVENMRKRNKLYVIQTLEDTTKQNVLRVVAQDVKFSASQLDELYLLFKNHDPSLPYLDQYQLDQSQFSSLFNLLQPWTTHTHSRSLARSAFHLLDENGDGLVNFKEFICGLDILYNRSFTEKLKLLFKLHLQPDSAEDGVIRKCPERGRAKVDLQEYLKQWQEDLQRREENIKDLPRINQVQFISLTKTLYSVFHGDEEEESLYRAVARVTSLLLRMEEVGRKLQDSSPQKTPQTTPTSTSQPESSPTKPTSPESETPAESRSTHDQPESPVSQHETAPSHSDITPNSTSHPSTPTSSPTETSSPVLDTPTDTPSSPCTVRDGDWSFSFEQILASLLNEPSVVRFFERVVNTDTLITRARKNQLKDAH

Target ORF information:

RefSeq Version	NM_001127515.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio TBC1 domain family member 8B (tbc1d8b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127515.1

ORF Insert Sequence:

ATGTGGCTCA AACCCGAAGA GGTGCTTCTC AAAAACGCGC TGAAATTATG GGTGACGGAG 
AAATCCAACG ATTACTTCGT GCTGCAGCGC AGACGCGGAT ACGGAGAGGA CAGCGGGGGA 
TTGACAGGCT TGCTGGTCGG AACCTTGGAC ACAGTTCTTG ACTCCACAGC CAAAGTGGCC 
CCGTTTCGCA TTTTACACCA GACACCCGAC TCTCAAGTGT ACTGGACTAT CGCCTGTGGA 
GCATCTTTAG AGGAGATCTC GCAGCACTGG GACTGGCTGC AGCAGAACAT TGTCAGAACT 
CTCTCTGTGT TCGACTCTGG CGAAGACATC ACCAGTTTTG TTCAGGGAAA GATAAGGGGT 
CTGATAGCAG AGGAAGGCAC ATCTGCAGGT GATGAGGAGG ATCCTGAGCG CTTCCGGGAG 
GCAGTGCTTC GCTTTGAACG CCTGTTTGGT TTGCCGCAGA GAGAAAAGCT TGTGACATAT 
TTCTCATGCA GCTACTGGAG AGGACGGGTG CCGAACCAGG GCTGGATCTA CCTCAGCACC 
AACTTCCTGT GTTTCTACTC CTATATGCTT GGAAATGAAG TGAAACTGGT GTATCCGTGG 
GATGAAGTGA GCCGTCTGGA ACGCACGTCG AGTGTTTTAT TGGCAGAGAG TATCCGTGTG 
CGAGTGCGTG GAGAGGATCA TTTCTTCTCG ATGCTTCTGA GGCTTCAGCA GACATACCTG 
ATCATGCAGC AGCTGGCCGA CTACGCCATT GTGCGCTTCT TCGATAAAGA AACATTTCAT 
GCTGAACATC CACTAGCCAA CCCCCTTCAC ATCACGCAGA GGGCTTTGGA AATACATGCA 
CGGAATCAGT CTTTCCGATC GTTTTTCCGG TTGCCGCAGG AGGAGAACCT TTGTGAAGTG 
TATGAGAGTT TCCTCTGGGT GCCGTTTAGT CACGTCAACA CTCTTGGAAA AATCTGTGTC 
TCGGAAAACT ATCTGTGCTT TGCAAGCCAA GATGGGTCTC AGTGCCATCT CATAATACCT 
CTATGGGAGG TGTTCAGTGT GGAGCTGCCA GACCGCAGCA GTCGAGCTCT GACTGTGTGC 
CTTCGAGGCA AGAGAGCTTT GCGTTTCTCT GAGGTTCGGG ATTTTGAGCG ACTTGCTGCC 
ACCATCCGCA GAAAGTGTGG GACATTGGGG AGCCCTCAGC ACTGCATCAC TAATCCGGAT 
GAGGAAGGTG TGATGGTGGG ACAAAGTCAA GCTGTCAGCA CTGAAGCTCT AATGAACGTC 
TTTCATCCAC ATGATGCAGA AAACCTTGAC CCCAAAATGC TGAAAGAGCG GATGAAGGAG 
CAGTCGTGGC AGATCCACTT TGCTGAATAT GGGCGTGGAA CTGGAATGTT TTGCACCAAA 
AAAACACGTG ACCTCATTGT GCGAGGAGTT CCAGAGACTC TCAGAGGAGA ACTGTGGATG 
CTCTTCTCAG GTGCAGTACA TGACATGATC TCTCACCCTG GGTATTACGG GCGGCTGCTG 
GAAGATTGTA TGGGCAGCAG CTCACTCGCA TGTGATGAGA TTGAGAGAGA TCTGCATCGC 
TCTCTCCCAG AACACCCAGC ATTCCAGAGC GACACGGGAA TATCAGCTCT GCGCCGGGTC 
CTCACTGCCT ATGCTCACCG CAACCCCAAA ATCGGCTACT GCCAGGCGAT GAATATACTG 
ACGTCAGTGT TGCTGCTGTA TGCTAAAGAA GAGGAAGCCT TCTGGCTGCT GGTGGCCGTG 
TGTGAAAGGA TGCTTCCGGA TTACTTCAAT CGCAGAATTA TCGGTGCTCT GGTGGACCAG 
GCTGTGTTCG AGGAGCTGAT CCGTGAACAT CTCACCCAGC TGACTGAACA CATGACAGAC 
CTGTCCTTCT TCTCGTCAGT GTCTTTATCG TGGTTTCTGA CGCTCTTCAT CAGTGTACTG 
CCAATAGAGA GCGCTGTGAA CGTGGTCGAC TGTTTCTTCT ACGATGGAAT CAAAGCCATC 
CTGCAGCTGG GCCTCGCTGT CCTCGACTAC AACATGGACA ATCTGCTCTG CTGCAATGAT 
GACGCAGAAG CCGTCACTGT CCTTAACAGG TTTTTTGACA GTGTCACAAA TAAAGACAGT 
CCCTTGCCAG CAACTGTTCA GCAGGCATCT GCAACAGCCA ATGACAAATC AATACAAAAG 
GTTGACATCA GCGACCTCAT CAAAGAAGCA TATGAGAAAT ATGGAGACAT TCGAACAGAG 
GAAGTGGAAA ACATGCGAAA GAGGAACAAG CTTTATGTCA TTCAAACACT TGAAGATACA 
ACAAAACAAA ATGTATTGCG TGTGGTTGCT CAGGATGTAA AGTTCAGTGC TTCTCAGCTG 
GACGAGCTTT ACTTGCTTTT TAAGAACCAT GACCCCAGCC TGCCATATCT GGACCAGTAT 
CAGCTGGACC AGTCCCAGTT TAGCAGCCTG TTTAACCTCT TGCAGCCGTG GACCACACAC 
ACACACTCGC GCTCTCTGGC ACGGTCAGCC TTCCATCTTC TGGATGAGAA TGGAGATGGA 
CTTGTGAACT TTAAGGAATT CATTTGCGGC CTGGACATAT TATACAACAG ATCATTCACA 
GAAAAACTGA AACTTCTCTT CAAACTGCAC CTTCAACCAG ACTCTGCAGA GGACGGAGTG 
ATCAGAAAAT GTCCAGAGAG AGGCCGTGCC AAAGTAGATC TGCAGGAGTA TCTGAAACAG 
TGGCAGGAGG ACTTACAGCG GAGAGAGGAG AATATTAAAG ATCTGCCCAG AATTAATCAG 
GTGCAGTTCA TTAGTTTGAC CAAGACTCTG TACAGCGTTT TCCACGGCGA TGAGGAGGAG 
GAGAGCCTGT ACCGCGCTGT GGCACGAGTG ACCAGTCTTC TGTTGCGCAT GGAGGAAGTG 
GGACGGAAAC TACAAGACAG CAGCCCGCAG AAAACACCCC AAACTACACC CACCAGCACA 
TCTCAGCCAG AATCCTCCCC AACAAAACCC ACCAGCCCTG AATCCGAGAC CCCCGCTGAG 
AGTCGAAGCA CCCACGACCA ACCCGAGTCT CCTGTCAGTC AACACGAAAC TGCTCCATCT 
CACTCTGACA TCACCCCGAA CTCAACCTCG CACCCTTCCA CACCCACCAG CAGTCCCACT 
GAAACCAGCA GCCCAGTGCT GGACACGCCA ACAGACACCC CCAGCTCGCC CTGCACGGTC 
AGAGACGGAG ACTGGAGTTT CTCCTTCGAG CAGATTTTGG CTTCTCTCCT GAATGAACCA 
TCTGTCGTTC GCTTCTTTGA ACGGGTCGTT AACACGGACA CTTTGATCAC TCGCGCACGC 
AAAAACCAGC TCAAAGACGC ACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:DKEY-110K5.6 ( XM_005165180.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005165237.1 Danio rerio si:dkey-110k5.6 (si:dkey-110k5.6), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa10298
Clone ID Related Accession (Same CDS sequence)	XM_005165180.4 , XM_005165180.3
Accession Version	XM_005165180.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	TBC1 domain family member 8B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165180.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTCA AACCCGAAGA GGTGCTTCTC AAAAACGCGC TGAAATTATG GGTGACGGAG 
AAATCCAACG ATTACTTCGT GCTGCAGCGC AGACGCGGAT ACGGAGAGGA CAGCGGGGGA 
TTGACAGGCT TGCTGGTCGG AACCTTGGAC ACAGTTCTTG ACTCCACAGC CAAAGTGGCC 
CCGTTTCGCA TTTTACACCA GACACCCGAC TCTCAAGTGT ACTGGACTAT CGCCTGTGGA 
GCATCTTTAG AGGAGATCTC GCAGCACTGG GACTGGCTGC AGCAGAACAT TGTCAGAACT 
CTCTCTGTGT TCGACTCTGG CGAAGACATC ACCAGTTTTG TTCAGGGAAA GATAAGGGGT 
CTGATAGCAG AGGAAGGCAC ATCTGCAGGT GATGAGGAGG ATCCTGAGCG CTTCCGGGAG 
GCAGTGCTTC GCTTTGAACG CCTGTTTGGT TTGCCGCAGA GAGAAAAGCT TGTGACATAT 
TTCTCATGCA GCTACTGGAG AGGACGGGTG CCGAACCAGG GCTGGATCTA CCTCAGCACC 
AACTTCCTGT GTTTCTACTC CTATATGCTT GGAAATGAAG TGAAACTGGT GTATCCGTGG 
GATGAAGTGA GCCGTCTGGA ACGCACGTCG AGTGTTTTAT TGGCAGAGAG TATCCGTGTG 
CGAGTGCGTG GAGAGGATCA TTTCTTCTCG ATGCTTCTGA GGCTTCAGCA GACATACCTG 
ATCATGCAGC AGCTGGCCGA CTACGCCATT GTGCGCTTCT TCGATAAAGA AACATTTCAT 
GCTGAACATC CACTAGCCAA CCCCCTTCAC ATCACGCAGA GGGCTTTGGA AATACATGCA 
CGGAATCAGT CTTTCCGATC GTTTTTCCGG TTGCCGCAGG AGGAGAACCT TTGTGAAGTG 
TATGAGAGTT TCCTCTGGGT GCCGTTTAGT CACGTCAACA CTCTTGGAAA AATCTGTGTC 
TCGGAAAACT ATCTGTGCTT TGCAAGCCAA GATGGGTCTC AGTGCCATCT CATAATACCT 
CTATGGGAGG TGTTCAGTGT GGAGCTGCCA GACCGCAGCA GTCGAGCTCT GACTGTGTGC 
CTTCGAGGCA AGAGAGCTTT GCGTTTCTCT GAGGTTCGGG ATTTTGAGCG ACTTGCTGCC 
ACCATCCGCA GAAAGTGTGG GACATTGGGG AGCCCTCAGC ACTGCATCAC TAATCCGGAT 
GAGGAAGGTG TGATGGTGGG ACAAAGTCAA GCTGTCAGCA CTGAAGCTCT AATGAACGTC 
TTTCATCCAC ATGATGCAGA AAACCTTGAC CCCAAAATGC TGAAAGAGCG GATGAAGGAG 
CAGTCGTGGC AGATCCACTT TGCTGAATAT GGGCGTGGAA CTGGAATGTT TTGCACCAAA 
AAAACACGTG ACCTCATTGT GCGAGGAGTT CCAGAGACTC TCAGAGGAGA ACTGTGGATG 
CTCTTCTCAG GTGCAGTACA TGACATGATC TCTCACCCTG GGTATTACGG GCGGCTGCTG 
GAAGATTGTA TGGGCAGCAG CTCACTCGCA TGTGATGAGA TTGAGAGAGA TCTGCATCGC 
TCTCTCCCAG AACACCCAGC ATTCCAGAGC GACACGGGAA TATCAGCTCT GCGCCGGGTC 
CTCACTGCCT ATGCTCACCG CAACCCCAAA ATCGGCTACT GCCAGGCGAT GAATATACTG 
ACGTCAGTGT TGCTGCTGTA TGCTAAAGAA GAGGAAGCCT TCTGGCTGCT GGTGGCCGTG 
TGTGAAAGGA TGCTTCCGGA TTACTTCAAT CGCAGAATTA TCGGTGCTCT GGTGGACCAG 
GCTGTGTTCG AGGAGCTGAT CCGTGAACAT CTCACCCAGC TGACTGAACA CATGACAGAC 
CTGTCCTTCT TCTCGTCAGT GTCTTTATCG TGGTTTCTGA CGCTCTTCAT CAGTGTACTG 
CCAATAGAGA GCGCTGTGAA CGTGGTCGAC TGTTTCTTCT ACGATGGAAT CAAAGCCATC 
CTGCAGCTGG GCCTCGCTGT CCTCGACTAC AACATGGACA ATCTGCTCTG CTGCAATGAT 
GACGCAGAAG CCGTCACTGT CCTTAACAGG TTTTTTGACA GTGTCACAAA TAAAGACAGT 
CCCTTGCCAG CAACTGTTCA GCAGGCATCT GCAACAGCCA ATGACAAATC AATACAAAAG 
GTTGACATCA GCGACCTCAT CAAAGAAGCA TATGAGAAAT ATGGAGACAT TCGAACAGAG 
GAAGTGGAAA ACATGCGAAA GAGGAACAAG CTTTATGTCA TTCAAACACT TGAAGATACA 
ACAAAACAAA ATGTATTGCG TGTGGTTGCT CAGGATGTAA AGTTCAGTGC TTCTCAGCTG 
GACGAGCTTT ACTTGCTTTT TAAGAGGCAG CATTTTGTGT GCTGTTACTG GTCAGTGTGT 
AGTCCTGCCC TGCAGAACCA TGACCCCAGC CTGCCATATC TGGACCAGTA TCAGCTGGAC 
CAGTCCCAGT TTAGCAGCCT GTTTAACCTC TTGCAGCCGT GGACCACACA CACACACTCG 
CGCTCTCTGG CACGGTCAGC CTTCCATCTT CTGGATGAGA ATGGAGATGG ACTTGTGAAC 
TTTAAGGAAT TCATTTGCGG CCTGGACATA TTATACAACA GATCATTCAC AGAAAAACTG 
AAACTTCTCT TCAAACTGCA CCTTCAACCA GGGAACCAGG AGTGTAAGAT TTTACCGTCG 
TCTAGATTTT TTCCTCCGGG TGAAGATCAC TCTTTCTCCA CTCGTCTTTC TGGCCGTGCC 
AAAGTAGATC TGCAGGAGTA TCTGAAACAG TGGCAGGAGG ACTTACAGCG GAGAGAGGAG 
AATATTAAAG ATCTGCCCAG AATTAATCAG GTGCAGTTCA TTAGTTTGAC CAAGACTCTG 
TACAGCGTTT TCCACGGCGA TGAGGAGGAG GAGAGCCTGT ACCGCGCTGT GGCACGAGTG 
ACCAGTCTTC TGTTGCGCAT GGAGGAAGTG GGACGGAAAC TACAAGACAG CAGCCCGCAG 
AAAACACCCC AAACTACACC CACCAGCACA TCTCAGCCAG AATCCTCCCC AACAAAACCC 
ACCAGCCCTG AATCCGAGAC CCCCGCTGAG AGTCGAAGCA CCCACGACCA ACCCGAGTCT 
CCTGTCAGTC AACACGAAAC TGCTCCATCT CACTCTGACA TCACCCCGAA CTCAACCTCG 
CACCCTTCCA CACCCACCAG CAGTCCCACT GAAACCAGCA GCCCAGTGCT GGACACGCCA 
ACAGACACCC CCAGCTCGCC CTGCACGGTC AGAGACGGAG ACTGGAGTTT CTCCTTCGAG 
CAGATTTTGG CTTCTCTCCT GAATGAACCA TCTGTCGTTC GCTTCTTTGA ACGGGTCGTT 
AACACGGACA CTTTGATCAC TCGCGCACGC AAAAACCAGC TCAAAGACGC ACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165237.1
CDS	133..3549
Translation	MWLKPEEVLLKNALKLWVTEKSNDYFVLQRRRGYGEDSGGLTGLLVGTLDTVLDSTAKVAPFRILHQTPDSQVYWTIACGASLEEISQHWDWLQQNIVRTLSVFDSGEDITSFVQGKIRGLIAEEGTSAGDEEDPERFREAVLRFERLFGLPQREKLVTYFSCSYWRGRVPNQGWIYLSTNFLCFYSYMLGNEVKLVYPWDEVSRLERTSSVLLAESIRVRVRGEDHFFSMLLRLQQTYLIMQQLADYAIVRFFDKETFHAEHPLANPLHITQRALEIHARNQSFRSFFRLPQEENLCEVYESFLWVPFSHVNTLGKICVSENYLCFASQDGSQCHLIIPLWEVFSVELPDRSSRALTVCLRGKRALRFSEVRDFERLAATIRRKCGTLGSPQHCITNPDEEGVMVGQSQAVSTEALMNVFHPHDAENLDPKMLKERMKEQSWQIHFAEYGRGTGMFCTKKTRDLIVRGVPETLRGELWMLFSGAVHDMISHPGYYGRLLEDCMGSSSLACDEIERDLHRSLPEHPAFQSDTGISALRRVLTAYAHRNPKIGYCQAMNILTSVLLLYAKEEEAFWLLVAVCERMLPDYFNRRIIGALVDQAVFEELIREHLTQLTEHMTDLSFFSSVSLSWFLTLFISVLPIESAVNVVDCFFYDGIKAILQLGLAVLDYNMDNLLCCNDDAEAVTVLNRFFDSVTNKDSPLPATVQQASATANDKSIQKVDISDLIKEAYEKYGDIRTEEVENMRKRNKLYVIQTLEDTTKQNVLRVVAQDVKFSASQLDELYLLFKRQHFVCCYWSVCSPALQNHDPSLPYLDQYQLDQSQFSSLFNLLQPWTTHTHSRSLARSAFHLLDENGDGLVNFKEFICGLDILYNRSFTEKLKLLFKLHLQPGNQECKILPSSRFFPPGEDHSFSTRLSGRAKVDLQEYLKQWQEDLQRREENIKDLPRINQVQFISLTKTLYSVFHGDEEEESLYRAVARVTSLLLRMEEVGRKLQDSSPQKTPQTTPTSTSQPESSPTKPTSPESETPAESRSTHDQPESPVSQHETAPSHSDITPNSTSHPSTPTSSPTETSSPVLDTPTDTPSSPCTVRDGDWSFSFEQILASLLNEPSVVRFFERVVNTDTLITRARKNQLKDAH

Target ORF information:

RefSeq Version	XM_005165180.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-110k5.6 (si:dkey-110k5.6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165180.4

ORF Insert Sequence:

ATGTGGCTCA AACCCGAAGA GGTGCTTCTC AAAAACGCGC TGAAATTATG GGTGACGGAG 
AAATCCAACG ATTACTTCGT GCTGCAGCGC AGACGCGGAT ACGGAGAGGA CAGCGGGGGA 
TTGACAGGCT TGCTGGTCGG AACCTTGGAC ACAGTTCTTG ACTCCACAGC CAAAGTGGCC 
CCGTTTCGCA TTTTACACCA GACACCCGAC TCTCAAGTGT ACTGGACTAT CGCCTGTGGA 
GCATCTTTAG AGGAGATCTC GCAGCACTGG GACTGGCTGC AGCAGAACAT TGTCAGAACT 
CTCTCTGTGT TCGACTCTGG CGAAGACATC ACCAGTTTTG TTCAGGGAAA GATAAGGGGT 
CTGATAGCAG AGGAAGGCAC ATCTGCAGGT GATGAGGAGG ATCCTGAGCG CTTCCGGGAG 
GCAGTGCTTC GCTTTGAACG CCTGTTTGGT TTGCCGCAGA GAGAAAAGCT TGTGACATAT 
TTCTCATGCA GCTACTGGAG AGGACGGGTG CCGAACCAGG GCTGGATCTA CCTCAGCACC 
AACTTCCTGT GTTTCTACTC CTATATGCTT GGAAATGAAG TGAAACTGGT GTATCCGTGG 
GATGAAGTGA GCCGTCTGGA ACGCACGTCG AGTGTTTTAT TGGCAGAGAG TATCCGTGTG 
CGAGTGCGTG GAGAGGATCA TTTCTTCTCG ATGCTTCTGA GGCTTCAGCA GACATACCTG 
ATCATGCAGC AGCTGGCCGA CTACGCCATT GTGCGCTTCT TCGATAAAGA AACATTTCAT 
GCTGAACATC CACTAGCCAA CCCCCTTCAC ATCACGCAGA GGGCTTTGGA AATACATGCA 
CGGAATCAGT CTTTCCGATC GTTTTTCCGG TTGCCGCAGG AGGAGAACCT TTGTGAAGTG 
TATGAGAGTT TCCTCTGGGT GCCGTTTAGT CACGTCAACA CTCTTGGAAA AATCTGTGTC 
TCGGAAAACT ATCTGTGCTT TGCAAGCCAA GATGGGTCTC AGTGCCATCT CATAATACCT 
CTATGGGAGG TGTTCAGTGT GGAGCTGCCA GACCGCAGCA GTCGAGCTCT GACTGTGTGC 
CTTCGAGGCA AGAGAGCTTT GCGTTTCTCT GAGGTTCGGG ATTTTGAGCG ACTTGCTGCC 
ACCATCCGCA GAAAGTGTGG GACATTGGGG AGCCCTCAGC ACTGCATCAC TAATCCGGAT 
GAGGAAGGTG TGATGGTGGG ACAAAGTCAA GCTGTCAGCA CTGAAGCTCT AATGAACGTC 
TTTCATCCAC ATGATGCAGA AAACCTTGAC CCCAAAATGC TGAAAGAGCG GATGAAGGAG 
CAGTCGTGGC AGATCCACTT TGCTGAATAT GGGCGTGGAA CTGGAATGTT TTGCACCAAA 
AAAACACGTG ACCTCATTGT GCGAGGAGTT CCAGAGACTC TCAGAGGAGA ACTGTGGATG 
CTCTTCTCAG GTGCAGTACA TGACATGATC TCTCACCCTG GGTATTACGG GCGGCTGCTG 
GAAGATTGTA TGGGCAGCAG CTCACTCGCA TGTGATGAGA TTGAGAGAGA TCTGCATCGC 
TCTCTCCCAG AACACCCAGC ATTCCAGAGC GACACGGGAA TATCAGCTCT GCGCCGGGTC 
CTCACTGCCT ATGCTCACCG CAACCCCAAA ATCGGCTACT GCCAGGCGAT GAATATACTG 
ACGTCAGTGT TGCTGCTGTA TGCTAAAGAA GAGGAAGCCT TCTGGCTGCT GGTGGCCGTG 
TGTGAAAGGA TGCTTCCGGA TTACTTCAAT CGCAGAATTA TCGGTGCTCT GGTGGACCAG 
GCTGTGTTCG AGGAGCTGAT CCGTGAACAT CTCACCCAGC TGACTGAACA CATGACAGAC 
CTGTCCTTCT TCTCGTCAGT GTCTTTATCG TGGTTTCTGA CGCTCTTCAT CAGTGTACTG 
CCAATAGAGA GCGCTGTGAA CGTGGTCGAC TGTTTCTTCT ACGATGGAAT CAAAGCCATC 
CTGCAGCTGG GCCTCGCTGT CCTCGACTAC AACATGGACA ATCTGCTCTG CTGCAATGAT 
GACGCAGAAG CCGTCACTGT CCTTAACAGG TTTTTTGACA GTGTCACAAA TAAAGACAGT 
CCCTTGCCAG CAACTGTTCA GCAGGCATCT GCAACAGCCA ATGACAAATC AATACAAAAG 
GTTGACATCA GCGACCTCAT CAAAGAAGCA TATGAGAAAT ATGGAGACAT TCGAACAGAG 
GAAGTGGAAA ACATGCGAAA GAGGAACAAG CTTTATGTCA TTCAAACACT TGAAGATACA 
ACAAAACAAA ATGTATTGCG TGTGGTTGCT CAGGATGTAA AGTTCAGTGC TTCTCAGCTG 
GACGAGCTTT ACTTGCTTTT TAAGAGGCAG CATTTTGTGT GCTGTTACTG GTCAGTGTGT 
AGTCCTGCCC TGCAGAACCA TGACCCCAGC CTGCCATATC TGGACCAGTA TCAGCTGGAC 
CAGTCCCAGT TTAGCAGCCT GTTTAACCTC TTGCAGCCGT GGACCACACA CACACACTCG 
CGCTCTCTGG CACGGTCAGC CTTCCATCTT CTGGATGAGA ATGGAGATGG ACTTGTGAAC 
TTTAAGGAAT TCATTTGCGG CCTGGACATA TTATACAACA GATCATTCAC AGAAAAACTG 
AAACTTCTCT TCAAACTGCA CCTTCAACCA GGGAACCAGG AGTGTAAGAT TTTACCGTCG 
TCTAGATTTT TTCCTCCGGG TGAAGATCAC TCTTTCTCCA CTCGTCTTTC TGGCCGTGCC 
AAAGTAGATC TGCAGGAGTA TCTGAAACAG TGGCAGGAGG ACTTACAGCG GAGAGAGGAG 
AATATTAAAG ATCTGCCCAG AATTAATCAG GTGCAGTTCA TTAGTTTGAC CAAGACTCTG 
TACAGCGTTT TCCACGGCGA TGAGGAGGAG GAGAGCCTGT ACCGCGCTGT GGCACGAGTG 
ACCAGTCTTC TGTTGCGCAT GGAGGAAGTG GGACGGAAAC TACAAGACAG CAGCCCGCAG 
AAAACACCCC AAACTACACC CACCAGCACA TCTCAGCCAG AATCCTCCCC AACAAAACCC 
ACCAGCCCTG AATCCGAGAC CCCCGCTGAG AGTCGAAGCA CCCACGACCA ACCCGAGTCT 
CCTGTCAGTC AACACGAAAC TGCTCCATCT CACTCTGACA TCACCCCGAA CTCAACCTCG 
CACCCTTCCA CACCCACCAG CAGTCCCACT GAAACCAGCA GCCCAGTGCT GGACACGCCA 
ACAGACACCC CCAGCTCGCC CTGCACGGTC AGAGACGGAG ACTGGAGTTT CTCCTTCGAG 
CAGATTTTGG CTTCTCTCCT GAATGAACCA TCTGTCGTTC GCTTCTTTGA ACGGGTCGTT 
AACACGGACA CTTTGATCAC TCGCGCACGC AAAAACCAGC TCAAAGACGC ACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:DKEY-110K5.6 ( XM_005165180.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005165237.1 Danio rerio si:dkey-110k5.6 (si:dkey-110k5.6), transcript variant X2, mRNA.

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CloneID	ODa10298
Clone ID Related Accession (Same CDS sequence)	XM_005165180.4 , XM_005165180.3
Accession Version	XM_005165180.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3417bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	TBC1 domain family member 8B isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165180.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTCA AACCCGAAGA GGTGCTTCTC AAAAACGCGC TGAAATTATG GGTGACGGAG 
AAATCCAACG ATTACTTCGT GCTGCAGCGC AGACGCGGAT ACGGAGAGGA CAGCGGGGGA 
TTGACAGGCT TGCTGGTCGG AACCTTGGAC ACAGTTCTTG ACTCCACAGC CAAAGTGGCC 
CCGTTTCGCA TTTTACACCA GACACCCGAC TCTCAAGTGT ACTGGACTAT CGCCTGTGGA 
GCATCTTTAG AGGAGATCTC GCAGCACTGG GACTGGCTGC AGCAGAACAT TGTCAGAACT 
CTCTCTGTGT TCGACTCTGG CGAAGACATC ACCAGTTTTG TTCAGGGAAA GATAAGGGGT 
CTGATAGCAG AGGAAGGCAC ATCTGCAGGT GATGAGGAGG ATCCTGAGCG CTTCCGGGAG 
GCAGTGCTTC GCTTTGAACG CCTGTTTGGT TTGCCGCAGA GAGAAAAGCT TGTGACATAT 
TTCTCATGCA GCTACTGGAG AGGACGGGTG CCGAACCAGG GCTGGATCTA CCTCAGCACC 
AACTTCCTGT GTTTCTACTC CTATATGCTT GGAAATGAAG TGAAACTGGT GTATCCGTGG 
GATGAAGTGA GCCGTCTGGA ACGCACGTCG AGTGTTTTAT TGGCAGAGAG TATCCGTGTG 
CGAGTGCGTG GAGAGGATCA TTTCTTCTCG ATGCTTCTGA GGCTTCAGCA GACATACCTG 
ATCATGCAGC AGCTGGCCGA CTACGCCATT GTGCGCTTCT TCGATAAAGA AACATTTCAT 
GCTGAACATC CACTAGCCAA CCCCCTTCAC ATCACGCAGA GGGCTTTGGA AATACATGCA 
CGGAATCAGT CTTTCCGATC GTTTTTCCGG TTGCCGCAGG AGGAGAACCT TTGTGAAGTG 
TATGAGAGTT TCCTCTGGGT GCCGTTTAGT CACGTCAACA CTCTTGGAAA AATCTGTGTC 
TCGGAAAACT ATCTGTGCTT TGCAAGCCAA GATGGGTCTC AGTGCCATCT CATAATACCT 
CTATGGGAGG TGTTCAGTGT GGAGCTGCCA GACCGCAGCA GTCGAGCTCT GACTGTGTGC 
CTTCGAGGCA AGAGAGCTTT GCGTTTCTCT GAGGTTCGGG ATTTTGAGCG ACTTGCTGCC 
ACCATCCGCA GAAAGTGTGG GACATTGGGG AGCCCTCAGC ACTGCATCAC TAATCCGGAT 
GAGGAAGGTG TGATGGTGGG ACAAAGTCAA GCTGTCAGCA CTGAAGCTCT AATGAACGTC 
TTTCATCCAC ATGATGCAGA AAACCTTGAC CCCAAAATGC TGAAAGAGCG GATGAAGGAG 
CAGTCGTGGC AGATCCACTT TGCTGAATAT GGGCGTGGAA CTGGAATGTT TTGCACCAAA 
AAAACACGTG ACCTCATTGT GCGAGGAGTT CCAGAGACTC TCAGAGGAGA ACTGTGGATG 
CTCTTCTCAG GTGCAGTACA TGACATGATC TCTCACCCTG GGTATTACGG GCGGCTGCTG 
GAAGATTGTA TGGGCAGCAG CTCACTCGCA TGTGATGAGA TTGAGAGAGA TCTGCATCGC 
TCTCTCCCAG AACACCCAGC ATTCCAGAGC GACACGGGAA TATCAGCTCT GCGCCGGGTC 
CTCACTGCCT ATGCTCACCG CAACCCCAAA ATCGGCTACT GCCAGGCGAT GAATATACTG 
ACGTCAGTGT TGCTGCTGTA TGCTAAAGAA GAGGAAGCCT TCTGGCTGCT GGTGGCCGTG 
TGTGAAAGGA TGCTTCCGGA TTACTTCAAT CGCAGAATTA TCGGTGCTCT GGTGGACCAG 
GCTGTGTTCG AGGAGCTGAT CCGTGAACAT CTCACCCAGC TGACTGAACA CATGACAGAC 
CTGTCCTTCT TCTCGTCAGT GTCTTTATCG TGGTTTCTGA CGCTCTTCAT CAGTGTACTG 
CCAATAGAGA GCGCTGTGAA CGTGGTCGAC TGTTTCTTCT ACGATGGAAT CAAAGCCATC 
CTGCAGCTGG GCCTCGCTGT CCTCGACTAC AACATGGACA ATCTGCTCTG CTGCAATGAT 
GACGCAGAAG CCGTCACTGT CCTTAACAGG TTTTTTGACA GTGTCACAAA TAAAGACAGT 
CCCTTGCCAG CAACTGTTCA GCAGGCATCT GCAACAGCCA ATGACAAATC AATACAAAAG 
GTTGACATCA GCGACCTCAT CAAAGAAGCA TATGAGAAAT ATGGAGACAT TCGAACAGAG 
GAAGTGGAAA ACATGCGAAA GAGGAACAAG CTTTATGTCA TTCAAACACT TGAAGATACA 
ACAAAACAAA ATGTATTGCG TGTGGTTGCT CAGGATGTAA AGTTCAGTGC TTCTCAGCTG 
GACGAGCTTT ACTTGCTTTT TAAGAGGCAG CATTTTGTGT GCTGTTACTG GTCAGTGTGT 
AGTCCTGCCC TGCAGAACCA TGACCCCAGC CTGCCATATC TGGACCAGTA TCAGCTGGAC 
CAGTCCCAGT TTAGCAGCCT GTTTAACCTC TTGCAGCCGT GGACCACACA CACACACTCG 
CGCTCTCTGG CACGGTCAGC CTTCCATCTT CTGGATGAGA ATGGAGATGG ACTTGTGAAC 
TTTAAGGAAT TCATTTGCGG CCTGGACATA TTATACAACA GATCATTCAC AGAAAAACTG 
AAACTTCTCT TCAAACTGCA CCTTCAACCA GGGAACCAGG AGTGTAAGAT TTTACCGTCG 
TCTAGATTTT TTCCTCCGGG TGAAGATCAC TCTTTCTCCA CTCGTCTTTC TGGCCGTGCC 
AAAGTAGATC TGCAGGAGTA TCTGAAACAG TGGCAGGAGG ACTTACAGCG GAGAGAGGAG 
AATATTAAAG ATCTGCCCAG AATTAATCAG GTGCAGTTCA TTAGTTTGAC CAAGACTCTG 
TACAGCGTTT TCCACGGCGA TGAGGAGGAG GAGAGCCTGT ACCGCGCTGT GGCACGAGTG 
ACCAGTCTTC TGTTGCGCAT GGAGGAAGTG GGACGGAAAC TACAAGACAG CAGCCCGCAG 
AAAACACCCC AAACTACACC CACCAGCACA TCTCAGCCAG AATCCTCCCC AACAAAACCC 
ACCAGCCCTG AATCCGAGAC CCCCGCTGAG AGTCGAAGCA CCCACGACCA ACCCGAGTCT 
CCTGTCAGTC AACACGAAAC TGCTCCATCT CACTCTGACA TCACCCCGAA CTCAACCTCG 
CACCCTTCCA CACCCACCAG CAGTCCCACT GAAACCAGCA GCCCAGTGCT GGACACGCCA 
ACAGACACCC CCAGCTCGCC CTGCACGGTC AGAGACGGAG ACTGGAGTTT CTCCTTCGAG 
CAGATTTTGG CTTCTCTCCT GAATGAACCA TCTGTCGTTC GCTTCTTTGA ACGGGTCGTT 
AACACGGACA CTTTGATCAC TCGCGCACGC AAAAACCAGC TCAAAGACGC ACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165237.1
CDS	142..3558
Translation	MWLKPEEVLLKNALKLWVTEKSNDYFVLQRRRGYGEDSGGLTGLLVGTLDTVLDSTAKVAPFRILHQTPDSQVYWTIACGASLEEISQHWDWLQQNIVRTLSVFDSGEDITSFVQGKIRGLIAEEGTSAGDEEDPERFREAVLRFERLFGLPQREKLVTYFSCSYWRGRVPNQGWIYLSTNFLCFYSYMLGNEVKLVYPWDEVSRLERTSSVLLAESIRVRVRGEDHFFSMLLRLQQTYLIMQQLADYAIVRFFDKETFHAEHPLANPLHITQRALEIHARNQSFRSFFRLPQEENLCEVYESFLWVPFSHVNTLGKICVSENYLCFASQDGSQCHLIIPLWEVFSVELPDRSSRALTVCLRGKRALRFSEVRDFERLAATIRRKCGTLGSPQHCITNPDEEGVMVGQSQAVSTEALMNVFHPHDAENLDPKMLKERMKEQSWQIHFAEYGRGTGMFCTKKTRDLIVRGVPETLRGELWMLFSGAVHDMISHPGYYGRLLEDCMGSSSLACDEIERDLHRSLPEHPAFQSDTGISALRRVLTAYAHRNPKIGYCQAMNILTSVLLLYAKEEEAFWLLVAVCERMLPDYFNRRIIGALVDQAVFEELIREHLTQLTEHMTDLSFFSSVSLSWFLTLFISVLPIESAVNVVDCFFYDGIKAILQLGLAVLDYNMDNLLCCNDDAEAVTVLNRFFDSVTNKDSPLPATVQQASATANDKSIQKVDISDLIKEAYEKYGDIRTEEVENMRKRNKLYVIQTLEDTTKQNVLRVVAQDVKFSASQLDELYLLFKRQHFVCCYWSVCSPALQNHDPSLPYLDQYQLDQSQFSSLFNLLQPWTTHTHSRSLARSAFHLLDENGDGLVNFKEFICGLDILYNRSFTEKLKLLFKLHLQPGNQECKILPSSRFFPPGEDHSFSTRLSGRAKVDLQEYLKQWQEDLQRREENIKDLPRINQVQFISLTKTLYSVFHGDEEEESLYRAVARVTSLLLRMEEVGRKLQDSSPQKTPQTTPTSTSQPESSPTKPTSPESETPAESRSTHDQPESPVSQHETAPSHSDITPNSTSHPSTPTSSPTETSSPVLDTPTDTPSSPCTVRDGDWSFSFEQILASLLNEPSVVRFFERVVNTDTLITRARKNQLKDAH

Target ORF information:

RefSeq Version	XM_005165180.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:dkey-110k5.6 (si:dkey-110k5.6), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165180.3

ORF Insert Sequence:

ATGTGGCTCA AACCCGAAGA GGTGCTTCTC AAAAACGCGC TGAAATTATG GGTGACGGAG 
AAATCCAACG ATTACTTCGT GCTGCAGCGC AGACGCGGAT ACGGAGAGGA CAGCGGGGGA 
TTGACAGGCT TGCTGGTCGG AACCTTGGAC ACAGTTCTTG ACTCCACAGC CAAAGTGGCC 
CCGTTTCGCA TTTTACACCA GACACCCGAC TCTCAAGTGT ACTGGACTAT CGCCTGTGGA 
GCATCTTTAG AGGAGATCTC GCAGCACTGG GACTGGCTGC AGCAGAACAT TGTCAGAACT 
CTCTCTGTGT TCGACTCTGG CGAAGACATC ACCAGTTTTG TTCAGGGAAA GATAAGGGGT 
CTGATAGCAG AGGAAGGCAC ATCTGCAGGT GATGAGGAGG ATCCTGAGCG CTTCCGGGAG 
GCAGTGCTTC GCTTTGAACG CCTGTTTGGT TTGCCGCAGA GAGAAAAGCT TGTGACATAT 
TTCTCATGCA GCTACTGGAG AGGACGGGTG CCGAACCAGG GCTGGATCTA CCTCAGCACC 
AACTTCCTGT GTTTCTACTC CTATATGCTT GGAAATGAAG TGAAACTGGT GTATCCGTGG 
GATGAAGTGA GCCGTCTGGA ACGCACGTCG AGTGTTTTAT TGGCAGAGAG TATCCGTGTG 
CGAGTGCGTG GAGAGGATCA TTTCTTCTCG ATGCTTCTGA GGCTTCAGCA GACATACCTG 
ATCATGCAGC AGCTGGCCGA CTACGCCATT GTGCGCTTCT TCGATAAAGA AACATTTCAT 
GCTGAACATC CACTAGCCAA CCCCCTTCAC ATCACGCAGA GGGCTTTGGA AATACATGCA 
CGGAATCAGT CTTTCCGATC GTTTTTCCGG TTGCCGCAGG AGGAGAACCT TTGTGAAGTG 
TATGAGAGTT TCCTCTGGGT GCCGTTTAGT CACGTCAACA CTCTTGGAAA AATCTGTGTC 
TCGGAAAACT ATCTGTGCTT TGCAAGCCAA GATGGGTCTC AGTGCCATCT CATAATACCT 
CTATGGGAGG TGTTCAGTGT GGAGCTGCCA GACCGCAGCA GTCGAGCTCT GACTGTGTGC 
CTTCGAGGCA AGAGAGCTTT GCGTTTCTCT GAGGTTCGGG ATTTTGAGCG ACTTGCTGCC 
ACCATCCGCA GAAAGTGTGG GACATTGGGG AGCCCTCAGC ACTGCATCAC TAATCCGGAT 
GAGGAAGGTG TGATGGTGGG ACAAAGTCAA GCTGTCAGCA CTGAAGCTCT AATGAACGTC 
TTTCATCCAC ATGATGCAGA AAACCTTGAC CCCAAAATGC TGAAAGAGCG GATGAAGGAG 
CAGTCGTGGC AGATCCACTT TGCTGAATAT GGGCGTGGAA CTGGAATGTT TTGCACCAAA 
AAAACACGTG ACCTCATTGT GCGAGGAGTT CCAGAGACTC TCAGAGGAGA ACTGTGGATG 
CTCTTCTCAG GTGCAGTACA TGACATGATC TCTCACCCTG GGTATTACGG GCGGCTGCTG 
GAAGATTGTA TGGGCAGCAG CTCACTCGCA TGTGATGAGA TTGAGAGAGA TCTGCATCGC 
TCTCTCCCAG AACACCCAGC ATTCCAGAGC GACACGGGAA TATCAGCTCT GCGCCGGGTC 
CTCACTGCCT ATGCTCACCG CAACCCCAAA ATCGGCTACT GCCAGGCGAT GAATATACTG 
ACGTCAGTGT TGCTGCTGTA TGCTAAAGAA GAGGAAGCCT TCTGGCTGCT GGTGGCCGTG 
TGTGAAAGGA TGCTTCCGGA TTACTTCAAT CGCAGAATTA TCGGTGCTCT GGTGGACCAG 
GCTGTGTTCG AGGAGCTGAT CCGTGAACAT CTCACCCAGC TGACTGAACA CATGACAGAC 
CTGTCCTTCT TCTCGTCAGT GTCTTTATCG TGGTTTCTGA CGCTCTTCAT CAGTGTACTG 
CCAATAGAGA GCGCTGTGAA CGTGGTCGAC TGTTTCTTCT ACGATGGAAT CAAAGCCATC 
CTGCAGCTGG GCCTCGCTGT CCTCGACTAC AACATGGACA ATCTGCTCTG CTGCAATGAT 
GACGCAGAAG CCGTCACTGT CCTTAACAGG TTTTTTGACA GTGTCACAAA TAAAGACAGT 
CCCTTGCCAG CAACTGTTCA GCAGGCATCT GCAACAGCCA ATGACAAATC AATACAAAAG 
GTTGACATCA GCGACCTCAT CAAAGAAGCA TATGAGAAAT ATGGAGACAT TCGAACAGAG 
GAAGTGGAAA ACATGCGAAA GAGGAACAAG CTTTATGTCA TTCAAACACT TGAAGATACA 
ACAAAACAAA ATGTATTGCG TGTGGTTGCT CAGGATGTAA AGTTCAGTGC TTCTCAGCTG 
GACGAGCTTT ACTTGCTTTT TAAGAGGCAG CATTTTGTGT GCTGTTACTG GTCAGTGTGT 
AGTCCTGCCC TGCAGAACCA TGACCCCAGC CTGCCATATC TGGACCAGTA TCAGCTGGAC 
CAGTCCCAGT TTAGCAGCCT GTTTAACCTC TTGCAGCCGT GGACCACACA CACACACTCG 
CGCTCTCTGG CACGGTCAGC CTTCCATCTT CTGGATGAGA ATGGAGATGG ACTTGTGAAC 
TTTAAGGAAT TCATTTGCGG CCTGGACATA TTATACAACA GATCATTCAC AGAAAAACTG 
AAACTTCTCT TCAAACTGCA CCTTCAACCA GGGAACCAGG AGTGTAAGAT TTTACCGTCG 
TCTAGATTTT TTCCTCCGGG TGAAGATCAC TCTTTCTCCA CTCGTCTTTC TGGCCGTGCC 
AAAGTAGATC TGCAGGAGTA TCTGAAACAG TGGCAGGAGG ACTTACAGCG GAGAGAGGAG 
AATATTAAAG ATCTGCCCAG AATTAATCAG GTGCAGTTCA TTAGTTTGAC CAAGACTCTG 
TACAGCGTTT TCCACGGCGA TGAGGAGGAG GAGAGCCTGT ACCGCGCTGT GGCACGAGTG 
ACCAGTCTTC TGTTGCGCAT GGAGGAAGTG GGACGGAAAC TACAAGACAG CAGCCCGCAG 
AAAACACCCC AAACTACACC CACCAGCACA TCTCAGCCAG AATCCTCCCC AACAAAACCC 
ACCAGCCCTG AATCCGAGAC CCCCGCTGAG AGTCGAAGCA CCCACGACCA ACCCGAGTCT 
CCTGTCAGTC AACACGAAAC TGCTCCATCT CACTCTGACA TCACCCCGAA CTCAACCTCG 
CACCCTTCCA CACCCACCAG CAGTCCCACT GAAACCAGCA GCCCAGTGCT GGACACGCCA 
ACAGACACCC CCAGCTCGCC CTGCACGGTC AGAGACGGAG ACTGGAGTTT CTCCTTCGAG 
CAGATTTTGG CTTCTCTCCT GAATGAACCA TCTGTCGTTC GCTTCTTTGA ACGGGTCGTT 
AACACGGACA CTTTGATCAC TCGCGCACGC AAAAACCAGC TCAAAGACGC ACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:DKEY-110K5.6 ( XM_009301353.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009299628.1 Danio rerio si:dkey-110k5.6 (si:dkey-110k5.6), transcript variant X1, mRNA.

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CloneID	ODa44934
Clone ID Related Accession (Same CDS sequence)	XM_009301353.3 , XM_009301353.2
Accession Version	XM_009301353.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	TBC1 domain family member 8B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301353.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTCA AACCCGAAGA GGTGCTTCTC AAAAACGCGC TGAAATTATG GGTGACGGAG 
AAATCCAACG ATTACTTCGT GCTGCAGCGC AGACGCGGAT ACGGAGAGGA CAGCGGGGGA 
TTGACAGGCT TGCTGGTCGG AACCTTGGAC ACAGTTCTTG ACTCCACAGC CAAAGTGGCC 
CCGTTTCGCA TTTTACACCA GACACCCGAC TCTCAAGTGT ACTGGACTAT CGCCTGTGGA 
GCATCTTTAG AGGAGATCTC GCAGCACTGG GACTGGCTGC AGCAGAACAT TGTCAGAACT 
CTCTCTGTGT TCGACTCTGG CGAAGACATC ACCAGTTTTG TTCAGGGAAA GATAAGGGGT 
CTGATAGCAG AGGAAGGCAC ATCTGCAGGT GATGAGGAGG ATCCTGAGCG CTTCCGGGAG 
GCAGTGCTTC GCTTTGAACG CCTGTTTGGT TTGCCGCAGA GAGAAAAGCT TGTGACATAT 
TTCTCATGCA GCTACTGGAG AGGACGGGTG CCGAACCAGG GCTGGATCTA CCTCAGCACC 
AACTTCCTGT GTTTCTACTC CTATATGCTT GGAAATGAAG TGAAACTGGT GTATCCGTGG 
GATGAAGTGA GCCGTCTGGA ACGCACGTCG AGTGTTTTAT TGGCAGAGAG TATCCGTGTG 
CGAGTGCGTG GAGAGGATCA TTTCTTCTCG ATGCTTCTGA GGCTTCAGCA GACATACCTG 
ATCATGCAGC AGCTGGCCGA CTACGCCATT GTGCGCTTCT TCGATAAAGA AACATTTCAT 
GCTGAACATC CACTAGCCAA CCCCCTTCAC ATCACGCAGA GGGCTTTGGA AATACATGCA 
CGGAATCAGT CTTTCCGATC GTTTTTCCGG TTGCCGCAGG AGGAGAACCT TTGTGAAGTG 
TATGAGAGTT TCCTCTGGGT GCCGTTTAGT CACGTCAACA CTCTTGGAAA AATCTGTGTC 
TCGGAAAACT ATCTGTGCTT TGCAAGCCAA GATGGGTCTC AGTGCCATCT CATAATACCT 
CTATGGGAGG TGTTCAGTGT GGAGCTGCCA GACCGCAGCA GTCGAGCTCT GACTGTGTGC 
CTTCGAGGCA AGAGAGCTTT GCGTTTCTCT GAGGTTCGGG ATTTTGAGCG ACTTGCTGCC 
ACCATCCGCA GAAAGTGTGG GACATTGGGG AGCCCTCAGC ACTGCATCAC TAATCCGGAT 
GAGGAAGGTG TGATGGTGGG ACAAAGTCAA GCTGTCAGCA CTGAAGCTCT AATGAACGTC 
TTTCATCCAC ATGATGCAGA AAACCTTGAC CCCAAAATGC TGAAAGAGCG GATGAAGGAG 
CAGTCGTGGC AGATCCACTT TGCTGAATAT GGGCGTGGAA CTGGAATGTT TTGCACCAAA 
AAAACACGTG ACCTCATTGT GCGAGGAGTT CCAGAGACTC TCAGAGGAGA ACTGTGGATG 
CTCTTCTCAG GTGCAGTACA TGACATGATC TCTCACCCTG GGTATTACGG GCGGCTGCTG 
GAAGATTGTA TGGGCAGCAG CTCACTCGCA TGTGATGAGA TTGAGAGAGA TCTGCATCGC 
TCTCTCCCAG AACACCCAGC ATTCCAGAGC GACACGGGAA TATCAGCTCT GCGCCGGGTC 
CTCACTGCCT ATGCTCACCG CAACCCCAAA ATCGGCTACT GCCAGGCGAT GAATATACTG 
ACGTCAGTGT TGCTGCTGTA TGCTAAAGAA GAGGAAGCCT TCTGGCTGCT GGTGGCCGTG 
TGTGAAAGGA TGCTTCCGGA TTACTTCAAT CGCAGAATTA TCGGTGCTCT GGTGGACCAG 
GCTGTGTTCG AGGAGCTGAT CCGTGAACAT CTCACCCAGC TGACTGAACA CATGACAGAC 
CTGTCCTTCT TCTCGTCAGT GTCTTTATCG TGGTTTCTGA CGCTCTTCAT CAGTGTACTG 
CCAATAGAGA GCGCTGTGAA CGTGGTCGAC TGTTTCTTCT ACGATGGAAT CAAAGCCATC 
CTGCAGCTGG GCCTCGCTGT CCTCGACTAC AACATGGACA ATCTGCTCTG CTGCAATGAT 
GACGCAGAAG CCGTCACTGT CCTTAACAGG TTTTTTGACA GTGTCACAAA TAAAGACAGT 
CCCTTGCCAG CAACTGTTCA GCAGGCATCT GCAACAGCCA ATGACAAATC AATACAAAAG 
GTTGACATCA GCGACCTCAT CAAAGAAGCA TATGAGAAAT ATGGAGACAT TCGAACAGAG 
GAAGTGGAAA ACATGCGAAA GAGGAACAAG CTTTATGTCA TTCAAACACT TGAAGATACA 
ACAAAACAAA ATGTATTGCG TGTGGTTGCT CAGGATGTAA AGTTCAGTGC TTCTCAGCTG 
GACGAGCTTT ACTTGCTTTT TAAGAGGCAG CATTTTGTGT GCTGTTACTG GTCAGTGTGT 
AGTCCTGCCC TGCAGAACCA TGACCCCAGC CTGCCATATC TGGACCAGTA TCAGCTGGAC 
CAGTCCCAGT TTAGCAGCCT GTTTAACCTC TTGCAGCCGT GGACCACACA CACACACTCG 
CGCTCTCTGG CACGGTCAGC CTTCCATCTT CTGGATGAGA ATGGAGATGG ACTTGTGAAC 
TTTAAGGAAT TCATTTGCGG CCTGGACATA TTATACAACA GATCATTCAC AGAAAAACTG 
AAACTTCTCT TCAAACTGCA CCTTCAACCA GGGAACCAGG AGTGTAAGAT TTTACCGTCG 
TCTAGATTTT TTCCTCCGGG TGAAGATCAC TCTTTCTCCA CTCGTCTTTC TGACTCTGCA 
GAGGACGGAG TGATCAGAAA ATGTCCAGAG AGAGGCCGTG CCAAAGTAGA TCTGCAGGAG 
TATCTGAAAC AGTGGCAGGA GGACTTACAG CGGAGAGAGG AGAATATTAA AGATCTGCCC 
AGAATTAATC AGGTGCAGTT CATTAGTTTG ACCAAGACTC TGTACAGCGT TTTCCACGGC 
GATGAGGAGG AGGAGAGCCT GTACCGCGCT GTGGCACGAG TGACCAGTCT TCTGTTGCGC 
ATGGAGGAAG TGGGACGGAA ACTACAAGAC AGCAGCCCGC AGAAAACACC CCAAACTACA 
CCCACCAGCA CATCTCAGCC AGAATCCTCC CCAACAAAAC CCACCAGCCC TGAATCCGAG 
ACCCCCGCTG AGAGTCGAAG CACCCACGAC CAACCCGAGT CTCCTGTCAG TCAACACGAA 
ACTGCTCCAT CTCACTCTGA CATCACCCCG AACTCAACCT CGCACCCTTC CACACCCACC 
AGCAGTCCCA CTGAAACCAG CAGCCCAGTG CTGGACACGC CAACAGACAC CCCCAGCTCG 
CCCTGCACGG TCAGAGACGG AGACTGGAGT TTCTCCTTCG AGCAGATTTT GGCTTCTCTC 
CTGAATGAAC CATCTGTCGT TCGCTTCTTT GAACGGGTCG TTAACACGGA CACTTTGATC 
ACTCGCGCAC GCAAAAACCA GCTCAAAGAC GCACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299628.1
CDS	133..3591
Translation	MWLKPEEVLLKNALKLWVTEKSNDYFVLQRRRGYGEDSGGLTGLLVGTLDTVLDSTAKVAPFRILHQTPDSQVYWTIACGASLEEISQHWDWLQQNIVRTLSVFDSGEDITSFVQGKIRGLIAEEGTSAGDEEDPERFREAVLRFERLFGLPQREKLVTYFSCSYWRGRVPNQGWIYLSTNFLCFYSYMLGNEVKLVYPWDEVSRLERTSSVLLAESIRVRVRGEDHFFSMLLRLQQTYLIMQQLADYAIVRFFDKETFHAEHPLANPLHITQRALEIHARNQSFRSFFRLPQEENLCEVYESFLWVPFSHVNTLGKICVSENYLCFASQDGSQCHLIIPLWEVFSVELPDRSSRALTVCLRGKRALRFSEVRDFERLAATIRRKCGTLGSPQHCITNPDEEGVMVGQSQAVSTEALMNVFHPHDAENLDPKMLKERMKEQSWQIHFAEYGRGTGMFCTKKTRDLIVRGVPETLRGELWMLFSGAVHDMISHPGYYGRLLEDCMGSSSLACDEIERDLHRSLPEHPAFQSDTGISALRRVLTAYAHRNPKIGYCQAMNILTSVLLLYAKEEEAFWLLVAVCERMLPDYFNRRIIGALVDQAVFEELIREHLTQLTEHMTDLSFFSSVSLSWFLTLFISVLPIESAVNVVDCFFYDGIKAILQLGLAVLDYNMDNLLCCNDDAEAVTVLNRFFDSVTNKDSPLPATVQQASATANDKSIQKVDISDLIKEAYEKYGDIRTEEVENMRKRNKLYVIQTLEDTTKQNVLRVVAQDVKFSASQLDELYLLFKRQHFVCCYWSVCSPALQNHDPSLPYLDQYQLDQSQFSSLFNLLQPWTTHTHSRSLARSAFHLLDENGDGLVNFKEFICGLDILYNRSFTEKLKLLFKLHLQPGNQECKILPSSRFFPPGEDHSFSTRLSDSAEDGVIRKCPERGRAKVDLQEYLKQWQEDLQRREENIKDLPRINQVQFISLTKTLYSVFHGDEEEESLYRAVARVTSLLLRMEEVGRKLQDSSPQKTPQTTPTSTSQPESSPTKPTSPESETPAESRSTHDQPESPVSQHETAPSHSDITPNSTSHPSTPTSSPTETSSPVLDTPTDTPSSPCTVRDGDWSFSFEQILASLLNEPSVVRFFERVVNTDTLITRARKNQLKDAH

Target ORF information:

RefSeq Version	XM_009301353.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-110k5.6 (si:dkey-110k5.6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301353.3

ORF Insert Sequence:

ATGTGGCTCA AACCCGAAGA GGTGCTTCTC AAAAACGCGC TGAAATTATG GGTGACGGAG 
AAATCCAACG ATTACTTCGT GCTGCAGCGC AGACGCGGAT ACGGAGAGGA CAGCGGGGGA 
TTGACAGGCT TGCTGGTCGG AACCTTGGAC ACAGTTCTTG ACTCCACAGC CAAAGTGGCC 
CCGTTTCGCA TTTTACACCA GACACCCGAC TCTCAAGTGT ACTGGACTAT CGCCTGTGGA 
GCATCTTTAG AGGAGATCTC GCAGCACTGG GACTGGCTGC AGCAGAACAT TGTCAGAACT 
CTCTCTGTGT TCGACTCTGG CGAAGACATC ACCAGTTTTG TTCAGGGAAA GATAAGGGGT 
CTGATAGCAG AGGAAGGCAC ATCTGCAGGT GATGAGGAGG ATCCTGAGCG CTTCCGGGAG 
GCAGTGCTTC GCTTTGAACG CCTGTTTGGT TTGCCGCAGA GAGAAAAGCT TGTGACATAT 
TTCTCATGCA GCTACTGGAG AGGACGGGTG CCGAACCAGG GCTGGATCTA CCTCAGCACC 
AACTTCCTGT GTTTCTACTC CTATATGCTT GGAAATGAAG TGAAACTGGT GTATCCGTGG 
GATGAAGTGA GCCGTCTGGA ACGCACGTCG AGTGTTTTAT TGGCAGAGAG TATCCGTGTG 
CGAGTGCGTG GAGAGGATCA TTTCTTCTCG ATGCTTCTGA GGCTTCAGCA GACATACCTG 
ATCATGCAGC AGCTGGCCGA CTACGCCATT GTGCGCTTCT TCGATAAAGA AACATTTCAT 
GCTGAACATC CACTAGCCAA CCCCCTTCAC ATCACGCAGA GGGCTTTGGA AATACATGCA 
CGGAATCAGT CTTTCCGATC GTTTTTCCGG TTGCCGCAGG AGGAGAACCT TTGTGAAGTG 
TATGAGAGTT TCCTCTGGGT GCCGTTTAGT CACGTCAACA CTCTTGGAAA AATCTGTGTC 
TCGGAAAACT ATCTGTGCTT TGCAAGCCAA GATGGGTCTC AGTGCCATCT CATAATACCT 
CTATGGGAGG TGTTCAGTGT GGAGCTGCCA GACCGCAGCA GTCGAGCTCT GACTGTGTGC 
CTTCGAGGCA AGAGAGCTTT GCGTTTCTCT GAGGTTCGGG ATTTTGAGCG ACTTGCTGCC 
ACCATCCGCA GAAAGTGTGG GACATTGGGG AGCCCTCAGC ACTGCATCAC TAATCCGGAT 
GAGGAAGGTG TGATGGTGGG ACAAAGTCAA GCTGTCAGCA CTGAAGCTCT AATGAACGTC 
TTTCATCCAC ATGATGCAGA AAACCTTGAC CCCAAAATGC TGAAAGAGCG GATGAAGGAG 
CAGTCGTGGC AGATCCACTT TGCTGAATAT GGGCGTGGAA CTGGAATGTT TTGCACCAAA 
AAAACACGTG ACCTCATTGT GCGAGGAGTT CCAGAGACTC TCAGAGGAGA ACTGTGGATG 
CTCTTCTCAG GTGCAGTACA TGACATGATC TCTCACCCTG GGTATTACGG GCGGCTGCTG 
GAAGATTGTA TGGGCAGCAG CTCACTCGCA TGTGATGAGA TTGAGAGAGA TCTGCATCGC 
TCTCTCCCAG AACACCCAGC ATTCCAGAGC GACACGGGAA TATCAGCTCT GCGCCGGGTC 
CTCACTGCCT ATGCTCACCG CAACCCCAAA ATCGGCTACT GCCAGGCGAT GAATATACTG 
ACGTCAGTGT TGCTGCTGTA TGCTAAAGAA GAGGAAGCCT TCTGGCTGCT GGTGGCCGTG 
TGTGAAAGGA TGCTTCCGGA TTACTTCAAT CGCAGAATTA TCGGTGCTCT GGTGGACCAG 
GCTGTGTTCG AGGAGCTGAT CCGTGAACAT CTCACCCAGC TGACTGAACA CATGACAGAC 
CTGTCCTTCT TCTCGTCAGT GTCTTTATCG TGGTTTCTGA CGCTCTTCAT CAGTGTACTG 
CCAATAGAGA GCGCTGTGAA CGTGGTCGAC TGTTTCTTCT ACGATGGAAT CAAAGCCATC 
CTGCAGCTGG GCCTCGCTGT CCTCGACTAC AACATGGACA ATCTGCTCTG CTGCAATGAT 
GACGCAGAAG CCGTCACTGT CCTTAACAGG TTTTTTGACA GTGTCACAAA TAAAGACAGT 
CCCTTGCCAG CAACTGTTCA GCAGGCATCT GCAACAGCCA ATGACAAATC AATACAAAAG 
GTTGACATCA GCGACCTCAT CAAAGAAGCA TATGAGAAAT ATGGAGACAT TCGAACAGAG 
GAAGTGGAAA ACATGCGAAA GAGGAACAAG CTTTATGTCA TTCAAACACT TGAAGATACA 
ACAAAACAAA ATGTATTGCG TGTGGTTGCT CAGGATGTAA AGTTCAGTGC TTCTCAGCTG 
GACGAGCTTT ACTTGCTTTT TAAGAGGCAG CATTTTGTGT GCTGTTACTG GTCAGTGTGT 
AGTCCTGCCC TGCAGAACCA TGACCCCAGC CTGCCATATC TGGACCAGTA TCAGCTGGAC 
CAGTCCCAGT TTAGCAGCCT GTTTAACCTC TTGCAGCCGT GGACCACACA CACACACTCG 
CGCTCTCTGG CACGGTCAGC CTTCCATCTT CTGGATGAGA ATGGAGATGG ACTTGTGAAC 
TTTAAGGAAT TCATTTGCGG CCTGGACATA TTATACAACA GATCATTCAC AGAAAAACTG 
AAACTTCTCT TCAAACTGCA CCTTCAACCA GGGAACCAGG AGTGTAAGAT TTTACCGTCG 
TCTAGATTTT TTCCTCCGGG TGAAGATCAC TCTTTCTCCA CTCGTCTTTC TGACTCTGCA 
GAGGACGGAG TGATCAGAAA ATGTCCAGAG AGAGGCCGTG CCAAAGTAGA TCTGCAGGAG 
TATCTGAAAC AGTGGCAGGA GGACTTACAG CGGAGAGAGG AGAATATTAA AGATCTGCCC 
AGAATTAATC AGGTGCAGTT CATTAGTTTG ACCAAGACTC TGTACAGCGT TTTCCACGGC 
GATGAGGAGG AGGAGAGCCT GTACCGCGCT GTGGCACGAG TGACCAGTCT TCTGTTGCGC 
ATGGAGGAAG TGGGACGGAA ACTACAAGAC AGCAGCCCGC AGAAAACACC CCAAACTACA 
CCCACCAGCA CATCTCAGCC AGAATCCTCC CCAACAAAAC CCACCAGCCC TGAATCCGAG 
ACCCCCGCTG AGAGTCGAAG CACCCACGAC CAACCCGAGT CTCCTGTCAG TCAACACGAA 
ACTGCTCCAT CTCACTCTGA CATCACCCCG AACTCAACCT CGCACCCTTC CACACCCACC 
AGCAGTCCCA CTGAAACCAG CAGCCCAGTG CTGGACACGC CAACAGACAC CCCCAGCTCG 
CCCTGCACGG TCAGAGACGG AGACTGGAGT TTCTCCTTCG AGCAGATTTT GGCTTCTCTC 
CTGAATGAAC CATCTGTCGT TCGCTTCTTT GAACGGGTCG TTAACACGGA CACTTTGATC 
ACTCGCGCAC GCAAAAACCA GCTCAAAGAC GCACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:DKEY-110K5.6 ( XM_009301353.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009299628.1 Danio rerio si:dkey-110k5.6 (si:dkey-110k5.6), transcript variant X1, mRNA.

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CloneID	ODa44934
Clone ID Related Accession (Same CDS sequence)	XM_009301353.3 , XM_009301353.2
Accession Version	XM_009301353.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	TBC1 domain family member 8B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301353.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTCA AACCCGAAGA GGTGCTTCTC AAAAACGCGC TGAAATTATG GGTGACGGAG 
AAATCCAACG ATTACTTCGT GCTGCAGCGC AGACGCGGAT ACGGAGAGGA CAGCGGGGGA 
TTGACAGGCT TGCTGGTCGG AACCTTGGAC ACAGTTCTTG ACTCCACAGC CAAAGTGGCC 
CCGTTTCGCA TTTTACACCA GACACCCGAC TCTCAAGTGT ACTGGACTAT CGCCTGTGGA 
GCATCTTTAG AGGAGATCTC GCAGCACTGG GACTGGCTGC AGCAGAACAT TGTCAGAACT 
CTCTCTGTGT TCGACTCTGG CGAAGACATC ACCAGTTTTG TTCAGGGAAA GATAAGGGGT 
CTGATAGCAG AGGAAGGCAC ATCTGCAGGT GATGAGGAGG ATCCTGAGCG CTTCCGGGAG 
GCAGTGCTTC GCTTTGAACG CCTGTTTGGT TTGCCGCAGA GAGAAAAGCT TGTGACATAT 
TTCTCATGCA GCTACTGGAG AGGACGGGTG CCGAACCAGG GCTGGATCTA CCTCAGCACC 
AACTTCCTGT GTTTCTACTC CTATATGCTT GGAAATGAAG TGAAACTGGT GTATCCGTGG 
GATGAAGTGA GCCGTCTGGA ACGCACGTCG AGTGTTTTAT TGGCAGAGAG TATCCGTGTG 
CGAGTGCGTG GAGAGGATCA TTTCTTCTCG ATGCTTCTGA GGCTTCAGCA GACATACCTG 
ATCATGCAGC AGCTGGCCGA CTACGCCATT GTGCGCTTCT TCGATAAAGA AACATTTCAT 
GCTGAACATC CACTAGCCAA CCCCCTTCAC ATCACGCAGA GGGCTTTGGA AATACATGCA 
CGGAATCAGT CTTTCCGATC GTTTTTCCGG TTGCCGCAGG AGGAGAACCT TTGTGAAGTG 
TATGAGAGTT TCCTCTGGGT GCCGTTTAGT CACGTCAACA CTCTTGGAAA AATCTGTGTC 
TCGGAAAACT ATCTGTGCTT TGCAAGCCAA GATGGGTCTC AGTGCCATCT CATAATACCT 
CTATGGGAGG TGTTCAGTGT GGAGCTGCCA GACCGCAGCA GTCGAGCTCT GACTGTGTGC 
CTTCGAGGCA AGAGAGCTTT GCGTTTCTCT GAGGTTCGGG ATTTTGAGCG ACTTGCTGCC 
ACCATCCGCA GAAAGTGTGG GACATTGGGG AGCCCTCAGC ACTGCATCAC TAATCCGGAT 
GAGGAAGGTG TGATGGTGGG ACAAAGTCAA GCTGTCAGCA CTGAAGCTCT AATGAACGTC 
TTTCATCCAC ATGATGCAGA AAACCTTGAC CCCAAAATGC TGAAAGAGCG GATGAAGGAG 
CAGTCGTGGC AGATCCACTT TGCTGAATAT GGGCGTGGAA CTGGAATGTT TTGCACCAAA 
AAAACACGTG ACCTCATTGT GCGAGGAGTT CCAGAGACTC TCAGAGGAGA ACTGTGGATG 
CTCTTCTCAG GTGCAGTACA TGACATGATC TCTCACCCTG GGTATTACGG GCGGCTGCTG 
GAAGATTGTA TGGGCAGCAG CTCACTCGCA TGTGATGAGA TTGAGAGAGA TCTGCATCGC 
TCTCTCCCAG AACACCCAGC ATTCCAGAGC GACACGGGAA TATCAGCTCT GCGCCGGGTC 
CTCACTGCCT ATGCTCACCG CAACCCCAAA ATCGGCTACT GCCAGGCGAT GAATATACTG 
ACGTCAGTGT TGCTGCTGTA TGCTAAAGAA GAGGAAGCCT TCTGGCTGCT GGTGGCCGTG 
TGTGAAAGGA TGCTTCCGGA TTACTTCAAT CGCAGAATTA TCGGTGCTCT GGTGGACCAG 
GCTGTGTTCG AGGAGCTGAT CCGTGAACAT CTCACCCAGC TGACTGAACA CATGACAGAC 
CTGTCCTTCT TCTCGTCAGT GTCTTTATCG TGGTTTCTGA CGCTCTTCAT CAGTGTACTG 
CCAATAGAGA GCGCTGTGAA CGTGGTCGAC TGTTTCTTCT ACGATGGAAT CAAAGCCATC 
CTGCAGCTGG GCCTCGCTGT CCTCGACTAC AACATGGACA ATCTGCTCTG CTGCAATGAT 
GACGCAGAAG CCGTCACTGT CCTTAACAGG TTTTTTGACA GTGTCACAAA TAAAGACAGT 
CCCTTGCCAG CAACTGTTCA GCAGGCATCT GCAACAGCCA ATGACAAATC AATACAAAAG 
GTTGACATCA GCGACCTCAT CAAAGAAGCA TATGAGAAAT ATGGAGACAT TCGAACAGAG 
GAAGTGGAAA ACATGCGAAA GAGGAACAAG CTTTATGTCA TTCAAACACT TGAAGATACA 
ACAAAACAAA ATGTATTGCG TGTGGTTGCT CAGGATGTAA AGTTCAGTGC TTCTCAGCTG 
GACGAGCTTT ACTTGCTTTT TAAGAGGCAG CATTTTGTGT GCTGTTACTG GTCAGTGTGT 
AGTCCTGCCC TGCAGAACCA TGACCCCAGC CTGCCATATC TGGACCAGTA TCAGCTGGAC 
CAGTCCCAGT TTAGCAGCCT GTTTAACCTC TTGCAGCCGT GGACCACACA CACACACTCG 
CGCTCTCTGG CACGGTCAGC CTTCCATCTT CTGGATGAGA ATGGAGATGG ACTTGTGAAC 
TTTAAGGAAT TCATTTGCGG CCTGGACATA TTATACAACA GATCATTCAC AGAAAAACTG 
AAACTTCTCT TCAAACTGCA CCTTCAACCA GGGAACCAGG AGTGTAAGAT TTTACCGTCG 
TCTAGATTTT TTCCTCCGGG TGAAGATCAC TCTTTCTCCA CTCGTCTTTC TGACTCTGCA 
GAGGACGGAG TGATCAGAAA ATGTCCAGAG AGAGGCCGTG CCAAAGTAGA TCTGCAGGAG 
TATCTGAAAC AGTGGCAGGA GGACTTACAG CGGAGAGAGG AGAATATTAA AGATCTGCCC 
AGAATTAATC AGGTGCAGTT CATTAGTTTG ACCAAGACTC TGTACAGCGT TTTCCACGGC 
GATGAGGAGG AGGAGAGCCT GTACCGCGCT GTGGCACGAG TGACCAGTCT TCTGTTGCGC 
ATGGAGGAAG TGGGACGGAA ACTACAAGAC AGCAGCCCGC AGAAAACACC CCAAACTACA 
CCCACCAGCA CATCTCAGCC AGAATCCTCC CCAACAAAAC CCACCAGCCC TGAATCCGAG 
ACCCCCGCTG AGAGTCGAAG CACCCACGAC CAACCCGAGT CTCCTGTCAG TCAACACGAA 
ACTGCTCCAT CTCACTCTGA CATCACCCCG AACTCAACCT CGCACCCTTC CACACCCACC 
AGCAGTCCCA CTGAAACCAG CAGCCCAGTG CTGGACACGC CAACAGACAC CCCCAGCTCG 
CCCTGCACGG TCAGAGACGG AGACTGGAGT TTCTCCTTCG AGCAGATTTT GGCTTCTCTC 
CTGAATGAAC CATCTGTCGT TCGCTTCTTT GAACGGGTCG TTAACACGGA CACTTTGATC 
ACTCGCGCAC GCAAAAACCA GCTCAAAGAC GCACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299628.1
CDS	141..3599
Translation	MWLKPEEVLLKNALKLWVTEKSNDYFVLQRRRGYGEDSGGLTGLLVGTLDTVLDSTAKVAPFRILHQTPDSQVYWTIACGASLEEISQHWDWLQQNIVRTLSVFDSGEDITSFVQGKIRGLIAEEGTSAGDEEDPERFREAVLRFERLFGLPQREKLVTYFSCSYWRGRVPNQGWIYLSTNFLCFYSYMLGNEVKLVYPWDEVSRLERTSSVLLAESIRVRVRGEDHFFSMLLRLQQTYLIMQQLADYAIVRFFDKETFHAEHPLANPLHITQRALEIHARNQSFRSFFRLPQEENLCEVYESFLWVPFSHVNTLGKICVSENYLCFASQDGSQCHLIIPLWEVFSVELPDRSSRALTVCLRGKRALRFSEVRDFERLAATIRRKCGTLGSPQHCITNPDEEGVMVGQSQAVSTEALMNVFHPHDAENLDPKMLKERMKEQSWQIHFAEYGRGTGMFCTKKTRDLIVRGVPETLRGELWMLFSGAVHDMISHPGYYGRLLEDCMGSSSLACDEIERDLHRSLPEHPAFQSDTGISALRRVLTAYAHRNPKIGYCQAMNILTSVLLLYAKEEEAFWLLVAVCERMLPDYFNRRIIGALVDQAVFEELIREHLTQLTEHMTDLSFFSSVSLSWFLTLFISVLPIESAVNVVDCFFYDGIKAILQLGLAVLDYNMDNLLCCNDDAEAVTVLNRFFDSVTNKDSPLPATVQQASATANDKSIQKVDISDLIKEAYEKYGDIRTEEVENMRKRNKLYVIQTLEDTTKQNVLRVVAQDVKFSASQLDELYLLFKRQHFVCCYWSVCSPALQNHDPSLPYLDQYQLDQSQFSSLFNLLQPWTTHTHSRSLARSAFHLLDENGDGLVNFKEFICGLDILYNRSFTEKLKLLFKLHLQPGNQECKILPSSRFFPPGEDHSFSTRLSDSAEDGVIRKCPERGRAKVDLQEYLKQWQEDLQRREENIKDLPRINQVQFISLTKTLYSVFHGDEEEESLYRAVARVTSLLLRMEEVGRKLQDSSPQKTPQTTPTSTSQPESSPTKPTSPESETPAESRSTHDQPESPVSQHETAPSHSDITPNSTSHPSTPTSSPTETSSPVLDTPTDTPSSPCTVRDGDWSFSFEQILASLLNEPSVVRFFERVVNTDTLITRARKNQLKDAH

Target ORF information:

RefSeq Version	XM_009301353.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:dkey-110k5.6 (si:dkey-110k5.6), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301353.2

ORF Insert Sequence:

ATGTGGCTCA AACCCGAAGA GGTGCTTCTC AAAAACGCGC TGAAATTATG GGTGACGGAG 
AAATCCAACG ATTACTTCGT GCTGCAGCGC AGACGCGGAT ACGGAGAGGA CAGCGGGGGA 
TTGACAGGCT TGCTGGTCGG AACCTTGGAC ACAGTTCTTG ACTCCACAGC CAAAGTGGCC 
CCGTTTCGCA TTTTACACCA GACACCCGAC TCTCAAGTGT ACTGGACTAT CGCCTGTGGA 
GCATCTTTAG AGGAGATCTC GCAGCACTGG GACTGGCTGC AGCAGAACAT TGTCAGAACT 
CTCTCTGTGT TCGACTCTGG CGAAGACATC ACCAGTTTTG TTCAGGGAAA GATAAGGGGT 
CTGATAGCAG AGGAAGGCAC ATCTGCAGGT GATGAGGAGG ATCCTGAGCG CTTCCGGGAG 
GCAGTGCTTC GCTTTGAACG CCTGTTTGGT TTGCCGCAGA GAGAAAAGCT TGTGACATAT 
TTCTCATGCA GCTACTGGAG AGGACGGGTG CCGAACCAGG GCTGGATCTA CCTCAGCACC 
AACTTCCTGT GTTTCTACTC CTATATGCTT GGAAATGAAG TGAAACTGGT GTATCCGTGG 
GATGAAGTGA GCCGTCTGGA ACGCACGTCG AGTGTTTTAT TGGCAGAGAG TATCCGTGTG 
CGAGTGCGTG GAGAGGATCA TTTCTTCTCG ATGCTTCTGA GGCTTCAGCA GACATACCTG 
ATCATGCAGC AGCTGGCCGA CTACGCCATT GTGCGCTTCT TCGATAAAGA AACATTTCAT 
GCTGAACATC CACTAGCCAA CCCCCTTCAC ATCACGCAGA GGGCTTTGGA AATACATGCA 
CGGAATCAGT CTTTCCGATC GTTTTTCCGG TTGCCGCAGG AGGAGAACCT TTGTGAAGTG 
TATGAGAGTT TCCTCTGGGT GCCGTTTAGT CACGTCAACA CTCTTGGAAA AATCTGTGTC 
TCGGAAAACT ATCTGTGCTT TGCAAGCCAA GATGGGTCTC AGTGCCATCT CATAATACCT 
CTATGGGAGG TGTTCAGTGT GGAGCTGCCA GACCGCAGCA GTCGAGCTCT GACTGTGTGC 
CTTCGAGGCA AGAGAGCTTT GCGTTTCTCT GAGGTTCGGG ATTTTGAGCG ACTTGCTGCC 
ACCATCCGCA GAAAGTGTGG GACATTGGGG AGCCCTCAGC ACTGCATCAC TAATCCGGAT 
GAGGAAGGTG TGATGGTGGG ACAAAGTCAA GCTGTCAGCA CTGAAGCTCT AATGAACGTC 
TTTCATCCAC ATGATGCAGA AAACCTTGAC CCCAAAATGC TGAAAGAGCG GATGAAGGAG 
CAGTCGTGGC AGATCCACTT TGCTGAATAT GGGCGTGGAA CTGGAATGTT TTGCACCAAA 
AAAACACGTG ACCTCATTGT GCGAGGAGTT CCAGAGACTC TCAGAGGAGA ACTGTGGATG 
CTCTTCTCAG GTGCAGTACA TGACATGATC TCTCACCCTG GGTATTACGG GCGGCTGCTG 
GAAGATTGTA TGGGCAGCAG CTCACTCGCA TGTGATGAGA TTGAGAGAGA TCTGCATCGC 
TCTCTCCCAG AACACCCAGC ATTCCAGAGC GACACGGGAA TATCAGCTCT GCGCCGGGTC 
CTCACTGCCT ATGCTCACCG CAACCCCAAA ATCGGCTACT GCCAGGCGAT GAATATACTG 
ACGTCAGTGT TGCTGCTGTA TGCTAAAGAA GAGGAAGCCT TCTGGCTGCT GGTGGCCGTG 
TGTGAAAGGA TGCTTCCGGA TTACTTCAAT CGCAGAATTA TCGGTGCTCT GGTGGACCAG 
GCTGTGTTCG AGGAGCTGAT CCGTGAACAT CTCACCCAGC TGACTGAACA CATGACAGAC 
CTGTCCTTCT TCTCGTCAGT GTCTTTATCG TGGTTTCTGA CGCTCTTCAT CAGTGTACTG 
CCAATAGAGA GCGCTGTGAA CGTGGTCGAC TGTTTCTTCT ACGATGGAAT CAAAGCCATC 
CTGCAGCTGG GCCTCGCTGT CCTCGACTAC AACATGGACA ATCTGCTCTG CTGCAATGAT 
GACGCAGAAG CCGTCACTGT CCTTAACAGG TTTTTTGACA GTGTCACAAA TAAAGACAGT 
CCCTTGCCAG CAACTGTTCA GCAGGCATCT GCAACAGCCA ATGACAAATC AATACAAAAG 
GTTGACATCA GCGACCTCAT CAAAGAAGCA TATGAGAAAT ATGGAGACAT TCGAACAGAG 
GAAGTGGAAA ACATGCGAAA GAGGAACAAG CTTTATGTCA TTCAAACACT TGAAGATACA 
ACAAAACAAA ATGTATTGCG TGTGGTTGCT CAGGATGTAA AGTTCAGTGC TTCTCAGCTG 
GACGAGCTTT ACTTGCTTTT TAAGAGGCAG CATTTTGTGT GCTGTTACTG GTCAGTGTGT 
AGTCCTGCCC TGCAGAACCA TGACCCCAGC CTGCCATATC TGGACCAGTA TCAGCTGGAC 
CAGTCCCAGT TTAGCAGCCT GTTTAACCTC TTGCAGCCGT GGACCACACA CACACACTCG 
CGCTCTCTGG CACGGTCAGC CTTCCATCTT CTGGATGAGA ATGGAGATGG ACTTGTGAAC 
TTTAAGGAAT TCATTTGCGG CCTGGACATA TTATACAACA GATCATTCAC AGAAAAACTG 
AAACTTCTCT TCAAACTGCA CCTTCAACCA GGGAACCAGG AGTGTAAGAT TTTACCGTCG 
TCTAGATTTT TTCCTCCGGG TGAAGATCAC TCTTTCTCCA CTCGTCTTTC TGACTCTGCA 
GAGGACGGAG TGATCAGAAA ATGTCCAGAG AGAGGCCGTG CCAAAGTAGA TCTGCAGGAG 
TATCTGAAAC AGTGGCAGGA GGACTTACAG CGGAGAGAGG AGAATATTAA AGATCTGCCC 
AGAATTAATC AGGTGCAGTT CATTAGTTTG ACCAAGACTC TGTACAGCGT TTTCCACGGC 
GATGAGGAGG AGGAGAGCCT GTACCGCGCT GTGGCACGAG TGACCAGTCT TCTGTTGCGC 
ATGGAGGAAG TGGGACGGAA ACTACAAGAC AGCAGCCCGC AGAAAACACC CCAAACTACA 
CCCACCAGCA CATCTCAGCC AGAATCCTCC CCAACAAAAC CCACCAGCCC TGAATCCGAG 
ACCCCCGCTG AGAGTCGAAG CACCCACGAC CAACCCGAGT CTCCTGTCAG TCAACACGAA 
ACTGCTCCAT CTCACTCTGA CATCACCCCG AACTCAACCT CGCACCCTTC CACACCCACC 
AGCAGTCCCA CTGAAACCAG CAGCCCAGTG CTGGACACGC CAACAGACAC CCCCAGCTCG 
CCCTGCACGG TCAGAGACGG AGACTGGAGT TTCTCCTTCG AGCAGATTTT GGCTTCTCTC 
CTGAATGAAC CATCTGTCGT TCGCTTCTTT GAACGGGTCG TTAACACGGA CACTTTGATC 
ACTCGCGCAC GCAAAAACCA GCTCAAAGAC GCACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:DKEY-110K5.6 ( XM_009301354.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009299629.1 Danio rerio si:dkey-110k5.6 (si:dkey-110k5.6), transcript variant X3, mRNA.

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CloneID	ODa44935
Clone ID Related Accession (Same CDS sequence)	XM_009301354.2
Accession Version	XM_009301354.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	TBC1 domain family member 8B isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301354.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTCA AACCCGAAGA GGTGCTTCTC AAAAACGCGC TGAAATTATG GGTGACGGAG 
AAATCCAACG ATTACTTCGT GCTGCAGCGC AGACGCGGAT ACGGAGAGGA CAGCGGGGGA 
TTGACAGGCT TGCTGGTCGG AACCTTGGAC ACAGTTCTTG ACTCCACAGC CAAAGTGGCC 
CCGTTTCGCA TTTTACACCA GACACCCGAC TCTCAAGTGT ACTGGACTAT CGCCTGTGGA 
GCATCTTTAG AGGAGATCTC GCAGCACTGG GACTGGCTGC AGCAGAACAT TGTCAGAACT 
CTCTCTGTGT TCGACTCTGG CGAAGACATC ACCAGTTTTG TTCAGGGAAA GATAAGGGGT 
CTGATAGCAG AGGAAGGCAC ATCTGCAGGT GATGAGGAGG ATCCTGAGCG CTTCCGGGAG 
GCAGTGCTTC GCTTTGAACG CCTGTTTGGT TTGCCGCAGA GAGAAAAGCT TGTGACATAT 
TTCTCATGCA GCTACTGGAG AGGACGGGTG CCGAACCAGG GCTGGATCTA CCTCAGCACC 
AACTTCCTGT GTTTCTACTC CTATATGCTT GGAAATGAAG TGAAACTGGT GTATCCGTGG 
GATGAAGTGA GCCGTCTGGA ACGCACGTCG AGTGTTTTAT TGGCAGAGAG TATCCGTGTG 
CGAGTGCGTG GAGAGGATCA TTTCTTCTCG ATGCTTCTGA GGCTTCAGCA GACATACCTG 
ATCATGCAGC AGCTGGCCGA CTACGCCATT GTGCGCTTCT TCGATAAAGA AACATTTCAT 
GCTGAACATC CACTAGCCAA CCCCCTTCAC ATCACGCAGA GGGCTTTGGA AATACATGCA 
CGGAATCAGT CTTTCCGATC GTTTTTCCGG TTGCCGCAGG AGGAGAACCT TTGTGAAGTG 
TATGAGAGTT TCCTCTGGGT GCCGTTTAGT CACGTCAACA CTCTTGGAAA AATCTGTGTC 
TCGGAAAACT ATCTGTGCTT TGCAAGCCAA GATGGGTCTC AGTGCCATCT CATAATACCT 
CTATGGGAGG TGTTCAGTGT GGAGCTGCCA GACCGCAGCA GTCGAGCTCT GACTGTGTGC 
CTTCGAGGCA AGAGAGCTTT GCGTTTCTCT GAGGTTCGGG ATTTTGAGCG ACTTGCTGCC 
ACCATCCGCA GAAAGTGTGG GACATTGGGG AGCCCTCAGC ACTGCATCAC TAATCCGGAT 
GAGGAAGGTG TGATGGTGGG ACAAAGTCAA GCTGTCAGCA CTGAAGCTCT AATGAACGTC 
TTTCATCCAC ATGATGCAGA AAACCTTGAC CCCAAAATGC TGAAAGAGCG GATGAAGGAG 
CAGTCGTGGC AGATCCACTT TGCTGAATAT GGGCGTGGAA CTGGAATGTT TTGCACCAAA 
AAAACACGTG ACCTCATTGT GCGAGGAGTT CCAGAGACTC TCAGAGGAGA ACTGTGGATG 
CTCTTCTCAG GTGCAGTACA TGACATGATC TCTCACCCTG GGTATTACGG GCGGCTGCTG 
GAAGATTGTA TGGGCAGCAG CTCACTCGCA TGTGATGAGA TTGAGAGAGA TCTGCATCGC 
TCTCTCCCAG AACACCCAGC ATTCCAGAGC GACACGGGAA TATCAGCTCT GCGCCGGGTC 
CTCACTGCCT ATGCTCACCG CAACCCCAAA ATCGGCTACT GCCAGGCGAT GAATATACTG 
ACGTCAGTGT TGCTGCTGTA TGCTAAAGAA GAGGAAGCCT TCTGGCTGCT GGTGGCCGTG 
TGTGAAAGGA TGCTTCCGGA TTACTTCAAT CGCAGAATTA TCGGTGCTCT GGTGGACCAG 
GCTGTGTTCG AGGAGCTGAT CCGTGAACAT CTCACCCAGC TGACTGAACA CATGACAGAC 
CTGTCCTTCT TCTCGTCAGT GTCTTTATCG TGGTTTCTGA CGCTCTTCAT CAGTGTACTG 
CCAATAGAGA GCGCTGTGAA CGTGGTCGAC TGTTTCTTCT ACGATGGAAT CAAAGCCATC 
CTGCAGCTGG GCCTCGCTGT CCTCGACTAC AACATGGACA ATCTGCTCTG CTGCAATGAT 
GACGCAGAAG CCGTCACTGT CCTTAACAGG TTTTTTGACA GTGTCACAAA TAAAGACAGT 
CCCTTGCCAG CAACTGTTCA GCAGGCATCT GCAACAGCCA ATGACAAATC AATACAAAAG 
GTTGACATCA GCGACCTCAT CAAAGAAGCA TATGAGAAAT ATGGAGACAT TCGAACAGAG 
GAAGTGGAAA ACATGCGAAA GAGGAACAAG CTTTATGTCA TTCAAACACT TGAAGATACA 
ACAAAACAAA ATGTATTGCG TGTGGTTGCT CAGGATGTAA AGTTCAGTGC TTCTCAGCTG 
GACGAGCTTT ACTTGCTTTT TAAGAGGCAG CATTTTGTGT GCTGTTACTG GTCAGTGTGT 
AGTCCTGCCC TGCAGAACCA TGACCCCAGC CTGCCATATC TGGACCAGTA TCAGCTGGAC 
CAGTCCCAGT TTAGCAGCCT GTTTAACCTC TTGCAGCCGT GGACCACACA CACACACTCG 
CGCTCTCTGG CACGGTCAGC CTTCCATCTT CTGGATGAGA ATGGAGATGG ACTTGTGAAC 
TTTAAGGAAT TCATTTGCGG CCTGGACATA TTATACAACA GATCATTCAC AGAAAAACTG 
AAACTTCTCT TCAAACTGCA CCTTCAACCA GGGAACCAGG AGTACTCTGC AGAGGACGGA 
GTGATCAGAA AATGTCCAGA GAGAGGCCGT GCCAAAGTAG ATCTGCAGGA GTATCTGAAA 
CAGTGGCAGG AGGACTTACA GCGGAGAGAG GAGAATATTA AAGATCTGCC CAGAATTAAT 
CAGGTGCAGT TCATTAGTTT GACCAAGACT CTGTACAGCG TTTTCCACGG CGATGAGGAG 
GAGGAGAGCC TGTACCGCGC TGTGGCACGA GTGACCAGTC TTCTGTTGCG CATGGAGGAA 
GTGGGACGGA AACTACAAGA CAGCAGCCCG CAGAAAACAC CCCAAACTAC ACCCACCAGC 
ACATCTCAGC CAGAATCCTC CCCAACAAAA CCCACCAGCC CTGAATCCGA GACCCCCGCT 
GAGAGTCGAA GCACCCACGA CCAACCCGAG TCTCCTGTCA GTCAACACGA AACTGCTCCA 
TCTCACTCTG ACATCACCCC GAACTCAACC TCGCACCCTT CCACACCCAC CAGCAGTCCC 
ACTGAAACCA GCAGCCCAGT GCTGGACACG CCAACAGACA CCCCCAGCTC GCCCTGCACG 
GTCAGAGACG GAGACTGGAG TTTCTCCTTC GAGCAGATTT TGGCTTCTCT CCTGAATGAA 
CCATCTGTCG TTCGCTTCTT TGAACGGGTC GTTAACACGG ACACTTTGAT CACTCGCGCA 
CGCAAAAACC AGCTCAAAGA CGCACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299629.1
CDS	141..3530
Translation	MWLKPEEVLLKNALKLWVTEKSNDYFVLQRRRGYGEDSGGLTGLLVGTLDTVLDSTAKVAPFRILHQTPDSQVYWTIACGASLEEISQHWDWLQQNIVRTLSVFDSGEDITSFVQGKIRGLIAEEGTSAGDEEDPERFREAVLRFERLFGLPQREKLVTYFSCSYWRGRVPNQGWIYLSTNFLCFYSYMLGNEVKLVYPWDEVSRLERTSSVLLAESIRVRVRGEDHFFSMLLRLQQTYLIMQQLADYAIVRFFDKETFHAEHPLANPLHITQRALEIHARNQSFRSFFRLPQEENLCEVYESFLWVPFSHVNTLGKICVSENYLCFASQDGSQCHLIIPLWEVFSVELPDRSSRALTVCLRGKRALRFSEVRDFERLAATIRRKCGTLGSPQHCITNPDEEGVMVGQSQAVSTEALMNVFHPHDAENLDPKMLKERMKEQSWQIHFAEYGRGTGMFCTKKTRDLIVRGVPETLRGELWMLFSGAVHDMISHPGYYGRLLEDCMGSSSLACDEIERDLHRSLPEHPAFQSDTGISALRRVLTAYAHRNPKIGYCQAMNILTSVLLLYAKEEEAFWLLVAVCERMLPDYFNRRIIGALVDQAVFEELIREHLTQLTEHMTDLSFFSSVSLSWFLTLFISVLPIESAVNVVDCFFYDGIKAILQLGLAVLDYNMDNLLCCNDDAEAVTVLNRFFDSVTNKDSPLPATVQQASATANDKSIQKVDISDLIKEAYEKYGDIRTEEVENMRKRNKLYVIQTLEDTTKQNVLRVVAQDVKFSASQLDELYLLFKRQHFVCCYWSVCSPALQNHDPSLPYLDQYQLDQSQFSSLFNLLQPWTTHTHSRSLARSAFHLLDENGDGLVNFKEFICGLDILYNRSFTEKLKLLFKLHLQPGNQEYSAEDGVIRKCPERGRAKVDLQEYLKQWQEDLQRREENIKDLPRINQVQFISLTKTLYSVFHGDEEEESLYRAVARVTSLLLRMEEVGRKLQDSSPQKTPQTTPTSTSQPESSPTKPTSPESETPAESRSTHDQPESPVSQHETAPSHSDITPNSTSHPSTPTSSPTETSSPVLDTPTDTPSSPCTVRDGDWSFSFEQILASLLNEPSVVRFFERVVNTDTLITRARKNQLKDAH

Target ORF information:

RefSeq Version	XM_009301354.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:dkey-110k5.6 (si:dkey-110k5.6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301354.2

ORF Insert Sequence:

ATGTGGCTCA AACCCGAAGA GGTGCTTCTC AAAAACGCGC TGAAATTATG GGTGACGGAG 
AAATCCAACG ATTACTTCGT GCTGCAGCGC AGACGCGGAT ACGGAGAGGA CAGCGGGGGA 
TTGACAGGCT TGCTGGTCGG AACCTTGGAC ACAGTTCTTG ACTCCACAGC CAAAGTGGCC 
CCGTTTCGCA TTTTACACCA GACACCCGAC TCTCAAGTGT ACTGGACTAT CGCCTGTGGA 
GCATCTTTAG AGGAGATCTC GCAGCACTGG GACTGGCTGC AGCAGAACAT TGTCAGAACT 
CTCTCTGTGT TCGACTCTGG CGAAGACATC ACCAGTTTTG TTCAGGGAAA GATAAGGGGT 
CTGATAGCAG AGGAAGGCAC ATCTGCAGGT GATGAGGAGG ATCCTGAGCG CTTCCGGGAG 
GCAGTGCTTC GCTTTGAACG CCTGTTTGGT TTGCCGCAGA GAGAAAAGCT TGTGACATAT 
TTCTCATGCA GCTACTGGAG AGGACGGGTG CCGAACCAGG GCTGGATCTA CCTCAGCACC 
AACTTCCTGT GTTTCTACTC CTATATGCTT GGAAATGAAG TGAAACTGGT GTATCCGTGG 
GATGAAGTGA GCCGTCTGGA ACGCACGTCG AGTGTTTTAT TGGCAGAGAG TATCCGTGTG 
CGAGTGCGTG GAGAGGATCA TTTCTTCTCG ATGCTTCTGA GGCTTCAGCA GACATACCTG 
ATCATGCAGC AGCTGGCCGA CTACGCCATT GTGCGCTTCT TCGATAAAGA AACATTTCAT 
GCTGAACATC CACTAGCCAA CCCCCTTCAC ATCACGCAGA GGGCTTTGGA AATACATGCA 
CGGAATCAGT CTTTCCGATC GTTTTTCCGG TTGCCGCAGG AGGAGAACCT TTGTGAAGTG 
TATGAGAGTT TCCTCTGGGT GCCGTTTAGT CACGTCAACA CTCTTGGAAA AATCTGTGTC 
TCGGAAAACT ATCTGTGCTT TGCAAGCCAA GATGGGTCTC AGTGCCATCT CATAATACCT 
CTATGGGAGG TGTTCAGTGT GGAGCTGCCA GACCGCAGCA GTCGAGCTCT GACTGTGTGC 
CTTCGAGGCA AGAGAGCTTT GCGTTTCTCT GAGGTTCGGG ATTTTGAGCG ACTTGCTGCC 
ACCATCCGCA GAAAGTGTGG GACATTGGGG AGCCCTCAGC ACTGCATCAC TAATCCGGAT 
GAGGAAGGTG TGATGGTGGG ACAAAGTCAA GCTGTCAGCA CTGAAGCTCT AATGAACGTC 
TTTCATCCAC ATGATGCAGA AAACCTTGAC CCCAAAATGC TGAAAGAGCG GATGAAGGAG 
CAGTCGTGGC AGATCCACTT TGCTGAATAT GGGCGTGGAA CTGGAATGTT TTGCACCAAA 
AAAACACGTG ACCTCATTGT GCGAGGAGTT CCAGAGACTC TCAGAGGAGA ACTGTGGATG 
CTCTTCTCAG GTGCAGTACA TGACATGATC TCTCACCCTG GGTATTACGG GCGGCTGCTG 
GAAGATTGTA TGGGCAGCAG CTCACTCGCA TGTGATGAGA TTGAGAGAGA TCTGCATCGC 
TCTCTCCCAG AACACCCAGC ATTCCAGAGC GACACGGGAA TATCAGCTCT GCGCCGGGTC 
CTCACTGCCT ATGCTCACCG CAACCCCAAA ATCGGCTACT GCCAGGCGAT GAATATACTG 
ACGTCAGTGT TGCTGCTGTA TGCTAAAGAA GAGGAAGCCT TCTGGCTGCT GGTGGCCGTG 
TGTGAAAGGA TGCTTCCGGA TTACTTCAAT CGCAGAATTA TCGGTGCTCT GGTGGACCAG 
GCTGTGTTCG AGGAGCTGAT CCGTGAACAT CTCACCCAGC TGACTGAACA CATGACAGAC 
CTGTCCTTCT TCTCGTCAGT GTCTTTATCG TGGTTTCTGA CGCTCTTCAT CAGTGTACTG 
CCAATAGAGA GCGCTGTGAA CGTGGTCGAC TGTTTCTTCT ACGATGGAAT CAAAGCCATC 
CTGCAGCTGG GCCTCGCTGT CCTCGACTAC AACATGGACA ATCTGCTCTG CTGCAATGAT 
GACGCAGAAG CCGTCACTGT CCTTAACAGG TTTTTTGACA GTGTCACAAA TAAAGACAGT 
CCCTTGCCAG CAACTGTTCA GCAGGCATCT GCAACAGCCA ATGACAAATC AATACAAAAG 
GTTGACATCA GCGACCTCAT CAAAGAAGCA TATGAGAAAT ATGGAGACAT TCGAACAGAG 
GAAGTGGAAA ACATGCGAAA GAGGAACAAG CTTTATGTCA TTCAAACACT TGAAGATACA 
ACAAAACAAA ATGTATTGCG TGTGGTTGCT CAGGATGTAA AGTTCAGTGC TTCTCAGCTG 
GACGAGCTTT ACTTGCTTTT TAAGAGGCAG CATTTTGTGT GCTGTTACTG GTCAGTGTGT 
AGTCCTGCCC TGCAGAACCA TGACCCCAGC CTGCCATATC TGGACCAGTA TCAGCTGGAC 
CAGTCCCAGT TTAGCAGCCT GTTTAACCTC TTGCAGCCGT GGACCACACA CACACACTCG 
CGCTCTCTGG CACGGTCAGC CTTCCATCTT CTGGATGAGA ATGGAGATGG ACTTGTGAAC 
TTTAAGGAAT TCATTTGCGG CCTGGACATA TTATACAACA GATCATTCAC AGAAAAACTG 
AAACTTCTCT TCAAACTGCA CCTTCAACCA GGGAACCAGG AGTACTCTGC AGAGGACGGA 
GTGATCAGAA AATGTCCAGA GAGAGGCCGT GCCAAAGTAG ATCTGCAGGA GTATCTGAAA 
CAGTGGCAGG AGGACTTACA GCGGAGAGAG GAGAATATTA AAGATCTGCC CAGAATTAAT 
CAGGTGCAGT TCATTAGTTT GACCAAGACT CTGTACAGCG TTTTCCACGG CGATGAGGAG 
GAGGAGAGCC TGTACCGCGC TGTGGCACGA GTGACCAGTC TTCTGTTGCG CATGGAGGAA 
GTGGGACGGA AACTACAAGA CAGCAGCCCG CAGAAAACAC CCCAAACTAC ACCCACCAGC 
ACATCTCAGC CAGAATCCTC CCCAACAAAA CCCACCAGCC CTGAATCCGA GACCCCCGCT 
GAGAGTCGAA GCACCCACGA CCAACCCGAG TCTCCTGTCA GTCAACACGA AACTGCTCCA 
TCTCACTCTG ACATCACCCC GAACTCAACC TCGCACCCTT CCACACCCAC CAGCAGTCCC 
ACTGAAACCA GCAGCCCAGT GCTGGACACG CCAACAGACA CCCCCAGCTC GCCCTGCACG 
GTCAGAGACG GAGACTGGAG TTTCTCCTTC GAGCAGATTT TGGCTTCTCT CCTGAATGAA 
CCATCTGTCG TTCGCTTCTT TGAACGGGTC GTTAACACGG ACACTTTGAT CACTCGCGCA 
CGCAAAAACC AGCTCAAAGA CGCACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:DKEY-110K5.6 ( XM_009301355.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009299630.1 Danio rerio si:dkey-110k5.6 (si:dkey-110k5.6), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa44936
Clone ID Related Accession (Same CDS sequence)	XM_009301355.2 , XM_009301355.3
Accession Version	XM_009301355.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	TBC1 domain family member 8B isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301355.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTCA AACCCGAAGA GGTGCTTCTC AAAAACGCGC TGAAATTATG GGTGACGGAG 
AAATCCAACG ATTACTTCGT GCTGCAGCGC AGACGCGGAT ACGGAGAGGA CAGCGGGGGA 
TTGACAGGCT TGCTGGTCGG AACCTTGGAC ACAGTTCTTG ACTCCACAGC CAAAGTGGCC 
CCGTTTCGCA TTTTACACCA GACACCCGAC TCTCAAGTGT ACTGGACTAT CGCCTGTGGA 
GCATCTTTAG AGGAGATCTC GCAGCACTGG GACTGGCTGC AGCAGAACAT TGTCAGAACT 
CTCTCTGTGT TCGACTCTGG CGAAGACATC ACCAGTTTTG TTCAGGGAAA GATAAGGGGT 
CTGATAGCAG AGGAAGGCAC ATCTGCAGGT GATGAGGAGG ATCCTGAGCG CTTCCGGGAG 
GCAGTGCTTC GCTTTGAACG CCTGTTTGGT TTGCCGCAGA GAGAAAAGCT TGTGACATAT 
TTCTCATGCA GCTACTGGAG AGGACGGGTG CCGAACCAGG GCTGGATCTA CCTCAGCACC 
AACTTCCTGT GTTTCTACTC CTATATGCTT GGAAATGAAG TGAAACTGGT GTATCCGTGG 
GATGAAGTGA GCCGTCTGGA ACGCACGTCG AGTGTTTTAT TGGCAGAGAG TATCCGTGTG 
CGAGTGCGTG GAGAGGATCA TTTCTTCTCG ATGCTTCTGA GGCTTCAGCA GACATACCTG 
ATCATGCAGC AGCTGGCCGA CTACGCCATT GTGCGCTTCT TCGATAAAGA AACATTTCAT 
GCTGAACATC CACTAGCCAA CCCCCTTCAC ATCACGCAGA GGGCTTTGGA AATACATGCA 
CGGAATCAGT CTTTCCGATC GTTTTTCCGG TTGCCGCAGG AGGAGAACCT TTGTGAAGTG 
TATGAGAGTT TCCTCTGGGT GCCGTTTAGT CACGTCAACA CTCTTGGAAA AATCTGTGTC 
TCGGAAAACT ATCTGTGCTT TGCAAGCCAA GATGGGTCTC AGTGCCATCT CATAATACCT 
CTATGGGAGG TGTTCAGTGT GGAGCTGCCA GACCGCAGCA GTCGAGCTCT GACTGTGTGC 
CTTCGAGGCA AGAGAGCTTT GCGTTTCTCT GAGGTTCGGG ATTTTGAGCG ACTTGCTGCC 
ACCATCCGCA GAAAGTGTGG GACATTGGGG AGCCCTCAGC ACTGCATCAC TAATCCGGAT 
GAGGAAGGTG TGATGGTGGG ACAAAGTCAA GCTGTCAGCA CTGAAGCTCT AATGAACGTC 
TTTCATCCAC ATGATGCAGA AAACCTTGAC CCCAAAATGC TGAAAGAGCG GATGAAGGAG 
CAGTCGTGGC AGATCCACTT TGCTGAATAT GGGCGTGGAA CTGGAATGTT TTGCACCAAA 
AAAACACGTG ACCTCATTGT GCGAGGAGTT CCAGAGACTC TCAGAGGAGA ACTGTGGATG 
CTCTTCTCAG GTGCAGTACA TGACATGATC TCTCACCCTG GGTATTACGG GCGGCTGCTG 
GAAGATTGTA TGGGCAGCAG CTCACTCGCA TGTGATGAGA TTGAGAGAGA TCTGCATCGC 
TCTCTCCCAG AACACCCAGC ATTCCAGAGC GACACGGGAA TATCAGCTCT GCGCCGGGTC 
CTCACTGCCT ATGCTCACCG CAACCCCAAA ATCGGCTACT GCCAGGCGAT GAATATACTG 
ACGTCAGTGT TGCTGCTGTA TGCTAAAGAA GAGGAAGCCT TCTGGCTGCT GGTGGCCGTG 
TGTGAAAGGA TGCTTCCGGA TTACTTCAAT CGCAGAATTA TCGGTGCTCT GGTGGACCAG 
GCTGTGTTCG AGGAGCTGAT CCGTGAACAT CTCACCCAGC TGACTGAACA CATGACAGAC 
CTGTCCTTCT TCTCGTCAGT GTCTTTATCG TGGTTTCTGA CGCTCTTCAT CAGTGTACTG 
CCAATAGAGA GCGCTGTGAA CGTGGTCGAC TGTTTCTTCT ACGATGGAAT CAAAGCCATC 
CTGCAGCTGG GCCTCGCTGT CCTCGACTAC AACATGGACA ATCTGCTCTG CTGCAATGAT 
GACGCAGAAG CCGTCACTGT CCTTAACAGG TTTTTTGACA GTGTCACAAA TAAAGACAGT 
CCCTTGCCAG CAACTGTTCA GCAGGCATCT GCAACAGCCA ATGACAAATC AATACAAAAG 
GTTGACATCA GCGACCTCAT CAAAGAAGCA TATGAGAAAT ATGGAGACAT TCGAACAGAG 
GAAGTGGAAA ACATGCGAAA GAGGAACAAG CTTTATGTCA TTCAAACACT TGAAGATACA 
ACAAAACAAA ATGTATTGCG TGTGGTTGCT CAGGATGTAA AGTTCAGTGC TTCTCAGCTG 
GACGAGCTTT ACTTGCTTTT TAAGAGGCAG CATTTTGTGT GCTGTTACTG GTCAGTGTGT 
AGTCCTGCCC TGCAGAACCA TGACCCCAGC CTGCCATATC TGGACCAGTA TCAGCTGGAC 
CAGTCCCAGT TTAGCAGCCT GTTTAACCTC TTGCAGCCGT GGACCACACA CACACACTCG 
CGCTCTCTGG CACGGTCAGC CTTCCATCTT CTGGATGAGA ATGGAGATGG ACTTGTGAAC 
TTTAAGGAAT TCATTTGCGG CCTGGACATA TTATACAACA GATCATTCAC AGAAAAACTG 
AAACTTCTCT TCAAACTGCA CCTTCAACCA GACTCTGCAG AGGACGGAGT GATCAGAAAA 
TGTCCAGAGA GAGGCCGTGC CAAAGTAGAT CTGCAGGAGT ATCTGAAACA GTGGCAGGAG 
GACTTACAGC GGAGAGAGGA GAATATTAAA GATCTGCCCA GAATTAATCA GGTGCAGTTC 
ATTAGTTTGA CCAAGACTCT GTACAGCGTT TTCCACGGCG ATGAGGAGGA GGAGAGCCTG 
TACCGCGCTG TGGCACGAGT GACCAGTCTT CTGTTGCGCA TGGAGGAAGT GGGACGGAAA 
CTACAAGACA GCAGCCCGCA GAAAACACCC CAAACTACAC CCACCAGCAC ATCTCAGCCA 
GAATCCTCCC CAACAAAACC CACCAGCCCT GAATCCGAGA CCCCCGCTGA GAGTCGAAGC 
ACCCACGACC AACCCGAGTC TCCTGTCAGT CAACACGAAA CTGCTCCATC TCACTCTGAC 
ATCACCCCGA ACTCAACCTC GCACCCTTCC ACACCCACCA GCAGTCCCAC TGAAACCAGC 
AGCCCAGTGC TGGACACGCC AACAGACACC CCCAGCTCGC CCTGCACGGT CAGAGACGGA 
GACTGGAGTT TCTCCTTCGA GCAGATTTTG GCTTCTCTCC TGAATGAACC ATCTGTCGTT 
CGCTTCTTTG AACGGGTCGT TAACACGGAC ACTTTGATCA CTCGCGCACG CAAAAACCAG 
CTCAAAGACG CACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299630.1
CDS	141..3518
Translation	MWLKPEEVLLKNALKLWVTEKSNDYFVLQRRRGYGEDSGGLTGLLVGTLDTVLDSTAKVAPFRILHQTPDSQVYWTIACGASLEEISQHWDWLQQNIVRTLSVFDSGEDITSFVQGKIRGLIAEEGTSAGDEEDPERFREAVLRFERLFGLPQREKLVTYFSCSYWRGRVPNQGWIYLSTNFLCFYSYMLGNEVKLVYPWDEVSRLERTSSVLLAESIRVRVRGEDHFFSMLLRLQQTYLIMQQLADYAIVRFFDKETFHAEHPLANPLHITQRALEIHARNQSFRSFFRLPQEENLCEVYESFLWVPFSHVNTLGKICVSENYLCFASQDGSQCHLIIPLWEVFSVELPDRSSRALTVCLRGKRALRFSEVRDFERLAATIRRKCGTLGSPQHCITNPDEEGVMVGQSQAVSTEALMNVFHPHDAENLDPKMLKERMKEQSWQIHFAEYGRGTGMFCTKKTRDLIVRGVPETLRGELWMLFSGAVHDMISHPGYYGRLLEDCMGSSSLACDEIERDLHRSLPEHPAFQSDTGISALRRVLTAYAHRNPKIGYCQAMNILTSVLLLYAKEEEAFWLLVAVCERMLPDYFNRRIIGALVDQAVFEELIREHLTQLTEHMTDLSFFSSVSLSWFLTLFISVLPIESAVNVVDCFFYDGIKAILQLGLAVLDYNMDNLLCCNDDAEAVTVLNRFFDSVTNKDSPLPATVQQASATANDKSIQKVDISDLIKEAYEKYGDIRTEEVENMRKRNKLYVIQTLEDTTKQNVLRVVAQDVKFSASQLDELYLLFKRQHFVCCYWSVCSPALQNHDPSLPYLDQYQLDQSQFSSLFNLLQPWTTHTHSRSLARSAFHLLDENGDGLVNFKEFICGLDILYNRSFTEKLKLLFKLHLQPDSAEDGVIRKCPERGRAKVDLQEYLKQWQEDLQRREENIKDLPRINQVQFISLTKTLYSVFHGDEEEESLYRAVARVTSLLLRMEEVGRKLQDSSPQKTPQTTPTSTSQPESSPTKPTSPESETPAESRSTHDQPESPVSQHETAPSHSDITPNSTSHPSTPTSSPTETSSPVLDTPTDTPSSPCTVRDGDWSFSFEQILASLLNEPSVVRFFERVVNTDTLITRARKNQLKDAH

Target ORF information:

RefSeq Version	XM_009301355.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:dkey-110k5.6 (si:dkey-110k5.6), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301355.2

ORF Insert Sequence:

ATGTGGCTCA AACCCGAAGA GGTGCTTCTC AAAAACGCGC TGAAATTATG GGTGACGGAG 
AAATCCAACG ATTACTTCGT GCTGCAGCGC AGACGCGGAT ACGGAGAGGA CAGCGGGGGA 
TTGACAGGCT TGCTGGTCGG AACCTTGGAC ACAGTTCTTG ACTCCACAGC CAAAGTGGCC 
CCGTTTCGCA TTTTACACCA GACACCCGAC TCTCAAGTGT ACTGGACTAT CGCCTGTGGA 
GCATCTTTAG AGGAGATCTC GCAGCACTGG GACTGGCTGC AGCAGAACAT TGTCAGAACT 
CTCTCTGTGT TCGACTCTGG CGAAGACATC ACCAGTTTTG TTCAGGGAAA GATAAGGGGT 
CTGATAGCAG AGGAAGGCAC ATCTGCAGGT GATGAGGAGG ATCCTGAGCG CTTCCGGGAG 
GCAGTGCTTC GCTTTGAACG CCTGTTTGGT TTGCCGCAGA GAGAAAAGCT TGTGACATAT 
TTCTCATGCA GCTACTGGAG AGGACGGGTG CCGAACCAGG GCTGGATCTA CCTCAGCACC 
AACTTCCTGT GTTTCTACTC CTATATGCTT GGAAATGAAG TGAAACTGGT GTATCCGTGG 
GATGAAGTGA GCCGTCTGGA ACGCACGTCG AGTGTTTTAT TGGCAGAGAG TATCCGTGTG 
CGAGTGCGTG GAGAGGATCA TTTCTTCTCG ATGCTTCTGA GGCTTCAGCA GACATACCTG 
ATCATGCAGC AGCTGGCCGA CTACGCCATT GTGCGCTTCT TCGATAAAGA AACATTTCAT 
GCTGAACATC CACTAGCCAA CCCCCTTCAC ATCACGCAGA GGGCTTTGGA AATACATGCA 
CGGAATCAGT CTTTCCGATC GTTTTTCCGG TTGCCGCAGG AGGAGAACCT TTGTGAAGTG 
TATGAGAGTT TCCTCTGGGT GCCGTTTAGT CACGTCAACA CTCTTGGAAA AATCTGTGTC 
TCGGAAAACT ATCTGTGCTT TGCAAGCCAA GATGGGTCTC AGTGCCATCT CATAATACCT 
CTATGGGAGG TGTTCAGTGT GGAGCTGCCA GACCGCAGCA GTCGAGCTCT GACTGTGTGC 
CTTCGAGGCA AGAGAGCTTT GCGTTTCTCT GAGGTTCGGG ATTTTGAGCG ACTTGCTGCC 
ACCATCCGCA GAAAGTGTGG GACATTGGGG AGCCCTCAGC ACTGCATCAC TAATCCGGAT 
GAGGAAGGTG TGATGGTGGG ACAAAGTCAA GCTGTCAGCA CTGAAGCTCT AATGAACGTC 
TTTCATCCAC ATGATGCAGA AAACCTTGAC CCCAAAATGC TGAAAGAGCG GATGAAGGAG 
CAGTCGTGGC AGATCCACTT TGCTGAATAT GGGCGTGGAA CTGGAATGTT TTGCACCAAA 
AAAACACGTG ACCTCATTGT GCGAGGAGTT CCAGAGACTC TCAGAGGAGA ACTGTGGATG 
CTCTTCTCAG GTGCAGTACA TGACATGATC TCTCACCCTG GGTATTACGG GCGGCTGCTG 
GAAGATTGTA TGGGCAGCAG CTCACTCGCA TGTGATGAGA TTGAGAGAGA TCTGCATCGC 
TCTCTCCCAG AACACCCAGC ATTCCAGAGC GACACGGGAA TATCAGCTCT GCGCCGGGTC 
CTCACTGCCT ATGCTCACCG CAACCCCAAA ATCGGCTACT GCCAGGCGAT GAATATACTG 
ACGTCAGTGT TGCTGCTGTA TGCTAAAGAA GAGGAAGCCT TCTGGCTGCT GGTGGCCGTG 
TGTGAAAGGA TGCTTCCGGA TTACTTCAAT CGCAGAATTA TCGGTGCTCT GGTGGACCAG 
GCTGTGTTCG AGGAGCTGAT CCGTGAACAT CTCACCCAGC TGACTGAACA CATGACAGAC 
CTGTCCTTCT TCTCGTCAGT GTCTTTATCG TGGTTTCTGA CGCTCTTCAT CAGTGTACTG 
CCAATAGAGA GCGCTGTGAA CGTGGTCGAC TGTTTCTTCT ACGATGGAAT CAAAGCCATC 
CTGCAGCTGG GCCTCGCTGT CCTCGACTAC AACATGGACA ATCTGCTCTG CTGCAATGAT 
GACGCAGAAG CCGTCACTGT CCTTAACAGG TTTTTTGACA GTGTCACAAA TAAAGACAGT 
CCCTTGCCAG CAACTGTTCA GCAGGCATCT GCAACAGCCA ATGACAAATC AATACAAAAG 
GTTGACATCA GCGACCTCAT CAAAGAAGCA TATGAGAAAT ATGGAGACAT TCGAACAGAG 
GAAGTGGAAA ACATGCGAAA GAGGAACAAG CTTTATGTCA TTCAAACACT TGAAGATACA 
ACAAAACAAA ATGTATTGCG TGTGGTTGCT CAGGATGTAA AGTTCAGTGC TTCTCAGCTG 
GACGAGCTTT ACTTGCTTTT TAAGAGGCAG CATTTTGTGT GCTGTTACTG GTCAGTGTGT 
AGTCCTGCCC TGCAGAACCA TGACCCCAGC CTGCCATATC TGGACCAGTA TCAGCTGGAC 
CAGTCCCAGT TTAGCAGCCT GTTTAACCTC TTGCAGCCGT GGACCACACA CACACACTCG 
CGCTCTCTGG CACGGTCAGC CTTCCATCTT CTGGATGAGA ATGGAGATGG ACTTGTGAAC 
TTTAAGGAAT TCATTTGCGG CCTGGACATA TTATACAACA GATCATTCAC AGAAAAACTG 
AAACTTCTCT TCAAACTGCA CCTTCAACCA GACTCTGCAG AGGACGGAGT GATCAGAAAA 
TGTCCAGAGA GAGGCCGTGC CAAAGTAGAT CTGCAGGAGT ATCTGAAACA GTGGCAGGAG 
GACTTACAGC GGAGAGAGGA GAATATTAAA GATCTGCCCA GAATTAATCA GGTGCAGTTC 
ATTAGTTTGA CCAAGACTCT GTACAGCGTT TTCCACGGCG ATGAGGAGGA GGAGAGCCTG 
TACCGCGCTG TGGCACGAGT GACCAGTCTT CTGTTGCGCA TGGAGGAAGT GGGACGGAAA 
CTACAAGACA GCAGCCCGCA GAAAACACCC CAAACTACAC CCACCAGCAC ATCTCAGCCA 
GAATCCTCCC CAACAAAACC CACCAGCCCT GAATCCGAGA CCCCCGCTGA GAGTCGAAGC 
ACCCACGACC AACCCGAGTC TCCTGTCAGT CAACACGAAA CTGCTCCATC TCACTCTGAC 
ATCACCCCGA ACTCAACCTC GCACCCTTCC ACACCCACCA GCAGTCCCAC TGAAACCAGC 
AGCCCAGTGC TGGACACGCC AACAGACACC CCCAGCTCGC CCTGCACGGT CAGAGACGGA 
GACTGGAGTT TCTCCTTCGA GCAGATTTTG GCTTCTCTCC TGAATGAACC ATCTGTCGTT 
CGCTTCTTTG AACGGGTCGT TAACACGGAC ACTTTGATCA CTCGCGCACG CAAAAACCAG 
CTCAAAGACG CACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SI:DKEY-110K5.6 ( XM_009301355.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009299630.1 Danio rerio si:dkey-110k5.6 (si:dkey-110k5.6), transcript variant X3, mRNA.

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CloneID	ODa44936
Clone ID Related Accession (Same CDS sequence)	XM_009301355.2 , XM_009301355.3
Accession Version	XM_009301355.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	TBC1 domain family member 8B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301355.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCTCA AACCCGAAGA GGTGCTTCTC AAAAACGCGC TGAAATTATG GGTGACGGAG 
AAATCCAACG ATTACTTCGT GCTGCAGCGC AGACGCGGAT ACGGAGAGGA CAGCGGGGGA 
TTGACAGGCT TGCTGGTCGG AACCTTGGAC ACAGTTCTTG ACTCCACAGC CAAAGTGGCC 
CCGTTTCGCA TTTTACACCA GACACCCGAC TCTCAAGTGT ACTGGACTAT CGCCTGTGGA 
GCATCTTTAG AGGAGATCTC GCAGCACTGG GACTGGCTGC AGCAGAACAT TGTCAGAACT 
CTCTCTGTGT TCGACTCTGG CGAAGACATC ACCAGTTTTG TTCAGGGAAA GATAAGGGGT 
CTGATAGCAG AGGAAGGCAC ATCTGCAGGT GATGAGGAGG ATCCTGAGCG CTTCCGGGAG 
GCAGTGCTTC GCTTTGAACG CCTGTTTGGT TTGCCGCAGA GAGAAAAGCT TGTGACATAT 
TTCTCATGCA GCTACTGGAG AGGACGGGTG CCGAACCAGG GCTGGATCTA CCTCAGCACC 
AACTTCCTGT GTTTCTACTC CTATATGCTT GGAAATGAAG TGAAACTGGT GTATCCGTGG 
GATGAAGTGA GCCGTCTGGA ACGCACGTCG AGTGTTTTAT TGGCAGAGAG TATCCGTGTG 
CGAGTGCGTG GAGAGGATCA TTTCTTCTCG ATGCTTCTGA GGCTTCAGCA GACATACCTG 
ATCATGCAGC AGCTGGCCGA CTACGCCATT GTGCGCTTCT TCGATAAAGA AACATTTCAT 
GCTGAACATC CACTAGCCAA CCCCCTTCAC ATCACGCAGA GGGCTTTGGA AATACATGCA 
CGGAATCAGT CTTTCCGATC GTTTTTCCGG TTGCCGCAGG AGGAGAACCT TTGTGAAGTG 
TATGAGAGTT TCCTCTGGGT GCCGTTTAGT CACGTCAACA CTCTTGGAAA AATCTGTGTC 
TCGGAAAACT ATCTGTGCTT TGCAAGCCAA GATGGGTCTC AGTGCCATCT CATAATACCT 
CTATGGGAGG TGTTCAGTGT GGAGCTGCCA GACCGCAGCA GTCGAGCTCT GACTGTGTGC 
CTTCGAGGCA AGAGAGCTTT GCGTTTCTCT GAGGTTCGGG ATTTTGAGCG ACTTGCTGCC 
ACCATCCGCA GAAAGTGTGG GACATTGGGG AGCCCTCAGC ACTGCATCAC TAATCCGGAT 
GAGGAAGGTG TGATGGTGGG ACAAAGTCAA GCTGTCAGCA CTGAAGCTCT AATGAACGTC 
TTTCATCCAC ATGATGCAGA AAACCTTGAC CCCAAAATGC TGAAAGAGCG GATGAAGGAG 
CAGTCGTGGC AGATCCACTT TGCTGAATAT GGGCGTGGAA CTGGAATGTT TTGCACCAAA 
AAAACACGTG ACCTCATTGT GCGAGGAGTT CCAGAGACTC TCAGAGGAGA ACTGTGGATG 
CTCTTCTCAG GTGCAGTACA TGACATGATC TCTCACCCTG GGTATTACGG GCGGCTGCTG 
GAAGATTGTA TGGGCAGCAG CTCACTCGCA TGTGATGAGA TTGAGAGAGA TCTGCATCGC 
TCTCTCCCAG AACACCCAGC ATTCCAGAGC GACACGGGAA TATCAGCTCT GCGCCGGGTC 
CTCACTGCCT ATGCTCACCG CAACCCCAAA ATCGGCTACT GCCAGGCGAT GAATATACTG 
ACGTCAGTGT TGCTGCTGTA TGCTAAAGAA GAGGAAGCCT TCTGGCTGCT GGTGGCCGTG 
TGTGAAAGGA TGCTTCCGGA TTACTTCAAT CGCAGAATTA TCGGTGCTCT GGTGGACCAG 
GCTGTGTTCG AGGAGCTGAT CCGTGAACAT CTCACCCAGC TGACTGAACA CATGACAGAC 
CTGTCCTTCT TCTCGTCAGT GTCTTTATCG TGGTTTCTGA CGCTCTTCAT CAGTGTACTG 
CCAATAGAGA GCGCTGTGAA CGTGGTCGAC TGTTTCTTCT ACGATGGAAT CAAAGCCATC 
CTGCAGCTGG GCCTCGCTGT CCTCGACTAC AACATGGACA ATCTGCTCTG CTGCAATGAT 
GACGCAGAAG CCGTCACTGT CCTTAACAGG TTTTTTGACA GTGTCACAAA TAAAGACAGT 
CCCTTGCCAG CAACTGTTCA GCAGGCATCT GCAACAGCCA ATGACAAATC AATACAAAAG 
GTTGACATCA GCGACCTCAT CAAAGAAGCA TATGAGAAAT ATGGAGACAT TCGAACAGAG 
GAAGTGGAAA ACATGCGAAA GAGGAACAAG CTTTATGTCA TTCAAACACT TGAAGATACA 
ACAAAACAAA ATGTATTGCG TGTGGTTGCT CAGGATGTAA AGTTCAGTGC TTCTCAGCTG 
GACGAGCTTT ACTTGCTTTT TAAGAGGCAG CATTTTGTGT GCTGTTACTG GTCAGTGTGT 
AGTCCTGCCC TGCAGAACCA TGACCCCAGC CTGCCATATC TGGACCAGTA TCAGCTGGAC 
CAGTCCCAGT TTAGCAGCCT GTTTAACCTC TTGCAGCCGT GGACCACACA CACACACTCG 
CGCTCTCTGG CACGGTCAGC CTTCCATCTT CTGGATGAGA ATGGAGATGG ACTTGTGAAC 
TTTAAGGAAT TCATTTGCGG CCTGGACATA TTATACAACA GATCATTCAC AGAAAAACTG 
AAACTTCTCT TCAAACTGCA CCTTCAACCA GACTCTGCAG AGGACGGAGT GATCAGAAAA 
TGTCCAGAGA GAGGCCGTGC CAAAGTAGAT CTGCAGGAGT ATCTGAAACA GTGGCAGGAG 
GACTTACAGC GGAGAGAGGA GAATATTAAA GATCTGCCCA GAATTAATCA GGTGCAGTTC 
ATTAGTTTGA CCAAGACTCT GTACAGCGTT TTCCACGGCG ATGAGGAGGA GGAGAGCCTG 
TACCGCGCTG TGGCACGAGT GACCAGTCTT CTGTTGCGCA TGGAGGAAGT GGGACGGAAA 
CTACAAGACA GCAGCCCGCA GAAAACACCC CAAACTACAC CCACCAGCAC ATCTCAGCCA 
GAATCCTCCC CAACAAAACC CACCAGCCCT GAATCCGAGA CCCCCGCTGA GAGTCGAAGC 
ACCCACGACC AACCCGAGTC TCCTGTCAGT CAACACGAAA CTGCTCCATC TCACTCTGAC 
ATCACCCCGA ACTCAACCTC GCACCCTTCC ACACCCACCA GCAGTCCCAC TGAAACCAGC 
AGCCCAGTGC TGGACACGCC AACAGACACC CCCAGCTCGC CCTGCACGGT CAGAGACGGA 
GACTGGAGTT TCTCCTTCGA GCAGATTTTG GCTTCTCTCC TGAATGAACC ATCTGTCGTT 
CGCTTCTTTG AACGGGTCGT TAACACGGAC ACTTTGATCA CTCGCGCACG CAAAAACCAG 
CTCAAAGACG CACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299630.1
CDS	133..3510
Translation	MWLKPEEVLLKNALKLWVTEKSNDYFVLQRRRGYGEDSGGLTGLLVGTLDTVLDSTAKVAPFRILHQTPDSQVYWTIACGASLEEISQHWDWLQQNIVRTLSVFDSGEDITSFVQGKIRGLIAEEGTSAGDEEDPERFREAVLRFERLFGLPQREKLVTYFSCSYWRGRVPNQGWIYLSTNFLCFYSYMLGNEVKLVYPWDEVSRLERTSSVLLAESIRVRVRGEDHFFSMLLRLQQTYLIMQQLADYAIVRFFDKETFHAEHPLANPLHITQRALEIHARNQSFRSFFRLPQEENLCEVYESFLWVPFSHVNTLGKICVSENYLCFASQDGSQCHLIIPLWEVFSVELPDRSSRALTVCLRGKRALRFSEVRDFERLAATIRRKCGTLGSPQHCITNPDEEGVMVGQSQAVSTEALMNVFHPHDAENLDPKMLKERMKEQSWQIHFAEYGRGTGMFCTKKTRDLIVRGVPETLRGELWMLFSGAVHDMISHPGYYGRLLEDCMGSSSLACDEIERDLHRSLPEHPAFQSDTGISALRRVLTAYAHRNPKIGYCQAMNILTSVLLLYAKEEEAFWLLVAVCERMLPDYFNRRIIGALVDQAVFEELIREHLTQLTEHMTDLSFFSSVSLSWFLTLFISVLPIESAVNVVDCFFYDGIKAILQLGLAVLDYNMDNLLCCNDDAEAVTVLNRFFDSVTNKDSPLPATVQQASATANDKSIQKVDISDLIKEAYEKYGDIRTEEVENMRKRNKLYVIQTLEDTTKQNVLRVVAQDVKFSASQLDELYLLFKRQHFVCCYWSVCSPALQNHDPSLPYLDQYQLDQSQFSSLFNLLQPWTTHTHSRSLARSAFHLLDENGDGLVNFKEFICGLDILYNRSFTEKLKLLFKLHLQPDSAEDGVIRKCPERGRAKVDLQEYLKQWQEDLQRREENIKDLPRINQVQFISLTKTLYSVFHGDEEEESLYRAVARVTSLLLRMEEVGRKLQDSSPQKTPQTTPTSTSQPESSPTKPTSPESETPAESRSTHDQPESPVSQHETAPSHSDITPNSTSHPSTPTSSPTETSSPVLDTPTDTPSSPCTVRDGDWSFSFEQILASLLNEPSVVRFFERVVNTDTLITRARKNQLKDAH

Target ORF information:

RefSeq Version	XM_009301355.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-110k5.6 (si:dkey-110k5.6), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301355.3

ORF Insert Sequence:

ATGTGGCTCA AACCCGAAGA GGTGCTTCTC AAAAACGCGC TGAAATTATG GGTGACGGAG 
AAATCCAACG ATTACTTCGT GCTGCAGCGC AGACGCGGAT ACGGAGAGGA CAGCGGGGGA 
TTGACAGGCT TGCTGGTCGG AACCTTGGAC ACAGTTCTTG ACTCCACAGC CAAAGTGGCC 
CCGTTTCGCA TTTTACACCA GACACCCGAC TCTCAAGTGT ACTGGACTAT CGCCTGTGGA 
GCATCTTTAG AGGAGATCTC GCAGCACTGG GACTGGCTGC AGCAGAACAT TGTCAGAACT 
CTCTCTGTGT TCGACTCTGG CGAAGACATC ACCAGTTTTG TTCAGGGAAA GATAAGGGGT 
CTGATAGCAG AGGAAGGCAC ATCTGCAGGT GATGAGGAGG ATCCTGAGCG CTTCCGGGAG 
GCAGTGCTTC GCTTTGAACG CCTGTTTGGT TTGCCGCAGA GAGAAAAGCT TGTGACATAT 
TTCTCATGCA GCTACTGGAG AGGACGGGTG CCGAACCAGG GCTGGATCTA CCTCAGCACC 
AACTTCCTGT GTTTCTACTC CTATATGCTT GGAAATGAAG TGAAACTGGT GTATCCGTGG 
GATGAAGTGA GCCGTCTGGA ACGCACGTCG AGTGTTTTAT TGGCAGAGAG TATCCGTGTG 
CGAGTGCGTG GAGAGGATCA TTTCTTCTCG ATGCTTCTGA GGCTTCAGCA GACATACCTG 
ATCATGCAGC AGCTGGCCGA CTACGCCATT GTGCGCTTCT TCGATAAAGA AACATTTCAT 
GCTGAACATC CACTAGCCAA CCCCCTTCAC ATCACGCAGA GGGCTTTGGA AATACATGCA 
CGGAATCAGT CTTTCCGATC GTTTTTCCGG TTGCCGCAGG AGGAGAACCT TTGTGAAGTG 
TATGAGAGTT TCCTCTGGGT GCCGTTTAGT CACGTCAACA CTCTTGGAAA AATCTGTGTC 
TCGGAAAACT ATCTGTGCTT TGCAAGCCAA GATGGGTCTC AGTGCCATCT CATAATACCT 
CTATGGGAGG TGTTCAGTGT GGAGCTGCCA GACCGCAGCA GTCGAGCTCT GACTGTGTGC 
CTTCGAGGCA AGAGAGCTTT GCGTTTCTCT GAGGTTCGGG ATTTTGAGCG ACTTGCTGCC 
ACCATCCGCA GAAAGTGTGG GACATTGGGG AGCCCTCAGC ACTGCATCAC TAATCCGGAT 
GAGGAAGGTG TGATGGTGGG ACAAAGTCAA GCTGTCAGCA CTGAAGCTCT AATGAACGTC 
TTTCATCCAC ATGATGCAGA AAACCTTGAC CCCAAAATGC TGAAAGAGCG GATGAAGGAG 
CAGTCGTGGC AGATCCACTT TGCTGAATAT GGGCGTGGAA CTGGAATGTT TTGCACCAAA 
AAAACACGTG ACCTCATTGT GCGAGGAGTT CCAGAGACTC TCAGAGGAGA ACTGTGGATG 
CTCTTCTCAG GTGCAGTACA TGACATGATC TCTCACCCTG GGTATTACGG GCGGCTGCTG 
GAAGATTGTA TGGGCAGCAG CTCACTCGCA TGTGATGAGA TTGAGAGAGA TCTGCATCGC 
TCTCTCCCAG AACACCCAGC ATTCCAGAGC GACACGGGAA TATCAGCTCT GCGCCGGGTC 
CTCACTGCCT ATGCTCACCG CAACCCCAAA ATCGGCTACT GCCAGGCGAT GAATATACTG 
ACGTCAGTGT TGCTGCTGTA TGCTAAAGAA GAGGAAGCCT TCTGGCTGCT GGTGGCCGTG 
TGTGAAAGGA TGCTTCCGGA TTACTTCAAT CGCAGAATTA TCGGTGCTCT GGTGGACCAG 
GCTGTGTTCG AGGAGCTGAT CCGTGAACAT CTCACCCAGC TGACTGAACA CATGACAGAC 
CTGTCCTTCT TCTCGTCAGT GTCTTTATCG TGGTTTCTGA CGCTCTTCAT CAGTGTACTG 
CCAATAGAGA GCGCTGTGAA CGTGGTCGAC TGTTTCTTCT ACGATGGAAT CAAAGCCATC 
CTGCAGCTGG GCCTCGCTGT CCTCGACTAC AACATGGACA ATCTGCTCTG CTGCAATGAT 
GACGCAGAAG CCGTCACTGT CCTTAACAGG TTTTTTGACA GTGTCACAAA TAAAGACAGT 
CCCTTGCCAG CAACTGTTCA GCAGGCATCT GCAACAGCCA ATGACAAATC AATACAAAAG 
GTTGACATCA GCGACCTCAT CAAAGAAGCA TATGAGAAAT ATGGAGACAT TCGAACAGAG 
GAAGTGGAAA ACATGCGAAA GAGGAACAAG CTTTATGTCA TTCAAACACT TGAAGATACA 
ACAAAACAAA ATGTATTGCG TGTGGTTGCT CAGGATGTAA AGTTCAGTGC TTCTCAGCTG 
GACGAGCTTT ACTTGCTTTT TAAGAGGCAG CATTTTGTGT GCTGTTACTG GTCAGTGTGT 
AGTCCTGCCC TGCAGAACCA TGACCCCAGC CTGCCATATC TGGACCAGTA TCAGCTGGAC 
CAGTCCCAGT TTAGCAGCCT GTTTAACCTC TTGCAGCCGT GGACCACACA CACACACTCG 
CGCTCTCTGG CACGGTCAGC CTTCCATCTT CTGGATGAGA ATGGAGATGG ACTTGTGAAC 
TTTAAGGAAT TCATTTGCGG CCTGGACATA TTATACAACA GATCATTCAC AGAAAAACTG 
AAACTTCTCT TCAAACTGCA CCTTCAACCA GACTCTGCAG AGGACGGAGT GATCAGAAAA 
TGTCCAGAGA GAGGCCGTGC CAAAGTAGAT CTGCAGGAGT ATCTGAAACA GTGGCAGGAG 
GACTTACAGC GGAGAGAGGA GAATATTAAA GATCTGCCCA GAATTAATCA GGTGCAGTTC 
ATTAGTTTGA CCAAGACTCT GTACAGCGTT TTCCACGGCG ATGAGGAGGA GGAGAGCCTG 
TACCGCGCTG TGGCACGAGT GACCAGTCTT CTGTTGCGCA TGGAGGAAGT GGGACGGAAA 
CTACAAGACA GCAGCCCGCA GAAAACACCC CAAACTACAC CCACCAGCAC ATCTCAGCCA 
GAATCCTCCC CAACAAAACC CACCAGCCCT GAATCCGAGA CCCCCGCTGA GAGTCGAAGC 
ACCCACGACC AACCCGAGTC TCCTGTCAGT CAACACGAAA CTGCTCCATC TCACTCTGAC 
ATCACCCCGA ACTCAACCTC GCACCCTTCC ACACCCACCA GCAGTCCCAC TGAAACCAGC 
AGCCCAGTGC TGGACACGCC AACAGACACC CCCAGCTCGC CCTGCACGGT CAGAGACGGA 
GACTGGAGTT TCTCCTTCGA GCAGATTTTG GCTTCTCTCC TGAATGAACC ATCTGTCGTT 
CGCTTCTTTG AACGGGTCGT TAACACGGAC ACTTTGATCA CTCGCGCACG CAAAAACCAG 
CTCAAAGACG CACACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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SI:DKEY-110K5.6 cDNA ORF clone, Danio rerio(Zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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