fam135a gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_005157008.3	XP_005157065.1	protein FAM135A isoform X7
XM_017358775.2	XP_017214264.1	protein FAM135A isoform X2
XM_017358777.2	XP_017214266.1	protein FAM135A isoform X6
XM_017358774.1	XP_017214263.1	protein FAM135A isoform X1
XM_009307481.2	XP_009305756.1	protein FAM135A isoform X4
XM_017358774.2	XP_017214263.1	protein FAM135A isoform X1
XM_005157006.4	XP_005157063.1	protein FAM135A isoform X3
XM_009307481.3	XP_009305756.1	protein FAM135A isoform X4
XM_005157008.4	XP_005157065.1	protein FAM135A isoform X7
NM_001145594.1	NP_001139066.1	protein FAM135A
XM_005157006.3	XP_005157063.1	protein FAM135A isoform X3
XM_017358776.1	XP_017214265.1	protein FAM135A isoform X5
XM_017358775.1	XP_017214264.1	protein FAM135A isoform X2
XM_017358777.1	XP_017214266.1	protein FAM135A isoform X6
XM_017358776.2	XP_017214265.1	protein FAM135A isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa54720	XM_017358774.2 Latest version!	Danio rerio family with sequence similarity 135, member A (fam135a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa54721	XM_017358775.2 Latest version!	Danio rerio family with sequence similarity 135, member A (fam135a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa54722	XM_017358776.2 Latest version!	Danio rerio family with sequence similarity 135, member A (fam135a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa54723	XM_017358777.2 Latest version!	Danio rerio family with sequence similarity 135, member A (fam135a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa18995	XM_005157008.4 Latest version!	Danio rerio family with sequence similarity 135, member A (fam135a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa18993	NM_001145594.1 Latest version!	Danio rerio family with sequence similarity 135 member A (fam135a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa18993	XM_005157006.4 Latest version!	Danio rerio family with sequence similarity 135, member A (fam135a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44872	XM_009307481.3 Latest version!	Danio rerio family with sequence similarity 135, member A (fam135a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa54720	XM_017358774.1	Danio rerio family with sequence similarity 135 member A (fam135a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa44872	XM_009307481.2	Danio rerio family with sequence similarity 135 member A (fam135a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa18995	XM_005157008.3	Danio rerio family with sequence similarity 135 member A (fam135a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa18993	XM_005157006.3	Danio rerio family with sequence similarity 135 member A (fam135a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa54721	XM_017358775.1	Danio rerio family with sequence similarity 135 member A (fam135a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa54723	XM_017358777.1	Danio rerio family with sequence similarity 135 member A (fam135a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa54722	XM_017358776.1	Danio rerio family with sequence similarity 135 member A (fam135a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

fam135a ( XM_017358774.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214263.1 Danio rerio family with sequence similarity 135 member A (fam135a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa54720
Clone ID Related Accession (Same CDS sequence)	XM_017358774.1 , XM_017358774.2
Accession Version	XM_017358774.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein FAM135A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGGT 
GTTTTCCTTC TGCAGGTAGA AGAGTCTTTA AATGAAGTTG ACTTTCAGCT GTGTTTGGAC 
CTGTTCTTCA CTGATGGAGA CTACTCGCCT GAGGAGCCCA GTTCTCTGCA GAGCATCAGC 
AGCCGTACGC TGCGTTTGCA CTTTAGCCTC CACAGAGGCA TCCACCACCA CGTCAACGTC 
ATGTTTGACT ACTTCCACCT GTCTGTCATC TCTGCCACAA TCCATGCTTC ACTGGTGGCT 
CTGCATCAGC CTCTAATAAG TTTCCCCCGG CCAGTAAAAA GCACCTGGTT GAATCGTAAT 
GCTCCGGCAC AGAGCAAAGA CTCCCTCATC CCACCGCTGG AGACTGTGGT GTTCGGCAGC 
ACCTACGTCA AGCAGCTGTC CCCTGATGGC AAAAGCTTCC TGGTATCGGA TCAGTGCCTC 
CAGCATGCCT TCAGCCTGCA CCAGAACATG TGTGCCAACC TGCTGCGGGC ATATCAGAGC 
CTGTTCTGTT ACTTAACCTC CCTGACCAAG AATCTGCCCG CATCCCAGCG CATGGAGCTT 
GCCAAGCCCA GCATGCAGGT CCTTTATGAA CGAGTTCTCA GAAGACCTTA CCCTCCAGGC 
CAAAGGCACG TCTACATAGA GGAACTTGAT GTGGAGGTCC GACTTCATGA GCTTTGTGAA 
CAAGTCAAGC AGAAAGCAGA GAGCCCAGAT GAGTTGGCTG AATTGGTGAA CATGAATTTG 
GCGCAACTAT GTTCCCTACT CATGGCTTTG TGGGGGAATT TCCTGGAAGT TGTGTCACTA 
AATGAGCGTG TTTTTGTGCT TTTGGCCCAG GAGCACCACA CACTACGGGT GCAAAGATTT 
GCTGAAGCTT TCTTTTGTCT TGAACACCCA CGACAGAGTG CACTGGCCTA CCAGGAACTG 
CATGCTCACA GTCACCAACA AATGACCACA GCTATCAAAA GCTCTTCCTA CTTCTTGTCC 
TTACCACCTC TTCCTGTTGA ATGTGCTGAT CTTGACGGGG ATGTGAACTC CCTACCAATC 
ATCTTTGAGG ACAGATATTT GGAGTCTGTT ACTGAAGATC GTGATGGTCC ATGGCTTGGA 
CTCCAAAACA ACAGGAGTGG CTCTGTGTCC GCCAAAGCAG ATAGATCCAA CTCAAAGGAC 
TGCATTGCCA GTGGAGCCCT CGGTCCGTAT CCAGATAAGC AGCAAGCTTC CTTTGCCACT 
GCTTGGCCTG AACAAAACGA GCCTTCCCTT AAATCCAAAG CCAAGTCCAT CAAGCTCAAG 
AAGAACACCA AGACCGAAAA CTCAAAGAAG CTGATCAGAC AGAGCTCGAA AGATTCTGTG 
GTGCTCACAG GCTACAAGAA CTTGAAGGTT CCTCACAGTG AAAAGACCAT TAAGTCCAAA 
GACATCGACC ATCAATGCAA TCAGAGAGAT GAGCTAGAGG ATGGAGGTAA ATTCCTTCTT 
CAGAATGAAA CCACGACCAC GCATTGTGCT CAGAATGCTA AAGACTGCAC ATCTGGCAAC 
CTTCAAGGTA ACGCCACAAT CCTAAGTGAC GGCTTTCCCA ATCTTATGAG TTTGCAAATG 
CAGGCCAGGC CCCAGGCAGG TACTGGAGCA GAGGCCGCCG CTGCCAGAGT AGATCCCACA 
CATCCACCTG CTGGCCTCAG ACATATTGAT GTCAAACCCA GTGACAAGGA CCCCTACCAG 
GGCGATAAGG TCACCGTTCT CTTACCGCAC CACCCAGAGC GCACCACCAA GAGGGATGTT 
CCAGAAATCA CACAGACTGA TTTCTCTGAG TTCAAGAGTC GAGTTGGAGA TGGTGCTACT 
TTCGTCGGAC CTTGTGGAGC GGCTTTGAGG AACGATAATA TTCCTCAAGC CTCCTCCAGA 
CTCTCGGATT CCGGAATTGA AAGCGAACCC AGCTCTGCGA CACAGCTAGC CCCGGTTTCT 
CCATCTCCTT TTGCTCCTAT TGGGATCGAA GCACCTACAG AGCTGATTCC TCACAGACCA 
CCTCCGCTCA GTCCTGCTTC CAGAGGAGCC ACCGCATTGG AAGGCCTCAA TCCTGAAAGC 
ACCAGCGGCG TAAGCGGCGT CCAATCTTCC CTCACCTCCA TCAACTCACT CCCTTCGGAT 
GATGAGGTGG AGACAGAGGC CACCAGCAGG AAGTCCAGCA TCCTGGTCCA GGAACAGAGT 
CTCATCTTCT CCGGAGAATC TCAAAGCCTC TTGCTGACAA ACTCTAGAGT CTTGGCTCAA 
CAAAAAGCCA GTCATGGAGA TGGGATGGAC TCTGCTTTGC AGTCCCGCTG TGAAACTTCT 
GCTTCTGACA GACTTGTCGG ATCTAACGAG ACAGATCTTC CAGGTGGTCC GGAAGCTTGT 
AGTGAACCAC AGACAGCAGC CCAGTCCAGT ACATCAGTCA CCAGCAGCAC AGACATGGTG 
AAGAAAGGCT TTGTAGAAAA CTACTTTGGC TCTCGGTCCA GTACTGATAT ATCTGAAATC 
AGCCCTTTAG AGACGGCAGC CATAACATTG GGTATTCAGA CATGCCCCAA TGCTAATGAT 
GAAGAGGATG AGGAGGAAGA GCAGGAGCAT GAGATGATTG AGAATGGTTA TTATGAGGAG 
ACCGATGGTG TGGTTTATGT TAATGGGCTC CCGGAGGATG AAGGGAGGTG TGAAGATGGA 
GGAGCGGAGG GAAGAACACT GTTTTATGAT CATCTTGGTT TAGAGCAGCT CCAGGAAACT 
TACATTCCCT CTGCCGGCAT TCGGACACGG CCTACTGGAC CAGCACCAAC CAGCAGCGAC 
TTATGCTGGT ACAAGAGCGC CCCCACTGCT CAGATGACGG CTTTTGTCCA AGCGAAAGAG 
GAGTTGAAAC AACTAAAGCT GCCAGGCTTC CTGTACAGTG AGGTGGCAGA TCTGGCATCC 
ACTGTGCCAT ATTTCAGCAT GGATGAGGAT GAAAACTGTG ATGATGGCAT ACACCTTATA 
GTGTGTGTAC ACGGCTTGGA TGGTAACAGT GCAGATTTGC GCCTGGTGAA GACTTATCTG 
GAGCTTGGGT TGCCTGGAGC TAGGATAGAT TTTCTGATGT CTGAGCGAAA TCAGAATGAC 
ACATTTGCTG ATTTTGAGTC CATGACCGAC AGACTTTTGG ATGAAATTGT GCAGTACATA 
CAAATCTACA ACCTCACAGT TTCCAAGATC AGTTTTGTTG GTCATTCTCT GGGAAATCTG 
ATCGTGCGCT CTGTGCTGAC GAGGCCCAGG TTTAAGTGTT ATCTCAGCCG ACTGCACACC 
TTTCTGTCTC TGTCTGGACC TCACCTCGGC ACACTGTACA ACAGCTCAGC ACTCGTCAAT 
ACTGGACTGT GGTTCATGCA GAAGTGGAAG AAGTCTGGCT CTCTGCTGCA GCTGACCTGT 
CGAGACCATT CAGACCCGCG TCAGACCTTT CTCTATAAGC TGAGCAAGAA AGCAGGCCTT 
CAGTTCTTCA AGAATGTGGT GTTGGTGGGC TCTCTTCAGG ACCGATACGT TCCTTATCAC 
TCCGCTCGAA TCGAGATGTG CAAAACAGCC TTAAAGGACA AACAGACTGG TCCGGTGTAT 
GCTGAGATGA TCGAGAACCT TCTCCTTCCT GTGCTTCAGA ACAAAGACTG TAACCTGGTG 
CGTTACGACG TCATCCACGC GCTGCCCAAC ACCGCCAACT CTCTGATCGG CCGCGCAGCT 
CACATCGCCG TCCTGGACTC TGAGATTTTC CTGGAAAAGT TCTTCCTGGT GGCAGGGCTC 
AAGTTTTTTC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214263.1
CDS	388..4422
Translation	MTEVQATVEFSVELHKFYNVDLFQRGFYQVRASMKVPPRIPQRVEASLLHPIGSDLAFPASVQDDVVCSKTFQILYKNEEIVVNDVFVFKVMMLLDAKKGVFLLQVEESLNEVDFQLCLDLFFTDGDYSPEEPSSLQSISSRTLRLHFSLHRGIHHHVNVMFDYFHLSVISATIHASLVALHQPLISFPRPVKSTWLNRNAPAQSKDSLIPPLETVVFGSTYVKQLSPDGKSFLVSDQCLQHAFSLHQNMCANLLRAYQSLFCYLTSLTKNLPASQRMELAKPSMQVLYERVLRRPYPPGQRHVYIEELDVEVRLHELCEQVKQKAESPDELAELVNMNLAQLCSLLMALWGNFLEVVSLNERVFVLLAQEHHTLRVQRFAEAFFCLEHPRQSALAYQELHAHSHQQMTTAIKSSSYFLSLPPLPVECADLDGDVNSLPIIFEDRYLESVTEDRDGPWLGLQNNRSGSVSAKADRSNSKDCIASGALGPYPDKQQASFATAWPEQNEPSLKSKAKSIKLKKNTKTENSKKLIRQSSKDSVVLTGYKNLKVPHSEKTIKSKDIDHQCNQRDELEDGGKFLLQNETTTTHCAQNAKDCTSGNLQGNATILSDGFPNLMSLQMQARPQAGTGAEAAAARVDPTHPPAGLRHIDVKPSDKDPYQGDKVTVLLPHHPERTTKRDVPEITQTDFSEFKSRVGDGATFVGPCGAALRNDNIPQASSRLSDSGIESEPSSATQLAPVSPSPFAPIGIEAPTELIPHRPPPLSPASRGATALEGLNPESTSGVSGVQSSLTSINSLPSDDEVETEATSRKSSILVQEQSLIFSGESQSLLLTNSRVLAQQKASHGDGMDSALQSRCETSASDRLVGSNETDLPGGPEACSEPQTAAQSSTSVTSSTDMVKKGFVENYFGSRSSTDISEISPLETAAITLGIQTCPNANDEEDEEEEQEHEMIENGYYEETDGVVYVNGLPEDEGRCEDGGAEGRTLFYDHLGLEQLQETYIPSAGIRTRPTGPAPTSSDLCWYKSAPTAQMTAFVQAKEELKQLKLPGFLYSEVADLASTVPYFSMDEDENCDDGIHLIVCVHGLDGNSADLRLVKTYLELGLPGARIDFLMSERNQNDTFADFESMTDRLLDEIVQYIQIYNLTVSKISFVGHSLGNLIVRSVLTRPRFKCYLSRLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSKKAGLQFFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQTGPVYAEMIENLLLPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEIFLEKFFLVAGLKFFQ

Target ORF information:

RefSeq Version	XM_017358774.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio family with sequence similarity 135 member A (fam135a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358774.1

ORF Insert Sequence:

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGGT 
GTTTTCCTTC TGCAGGTAGA AGAGTCTTTA AATGAAGTTG ACTTTCAGCT GTGTTTGGAC 
CTGTTCTTCA CTGATGGAGA CTACTCGCCT GAGGAGCCCA GTTCTCTGCA GAGCATCAGC 
AGCCGTACGC TGCGTTTGCA CTTTAGCCTC CACAGAGGCA TCCACCACCA CGTCAACGTC 
ATGTTTGACT ACTTCCACCT GTCTGTCATC TCTGCCACAA TCCATGCTTC ACTGGTGGCT 
CTGCATCAGC CTCTAATAAG TTTCCCCCGG CCAGTAAAAA GCACCTGGTT GAATCGTAAT 
GCTCCGGCAC AGAGCAAAGA CTCCCTCATC CCACCGCTGG AGACTGTGGT GTTCGGCAGC 
ACCTACGTCA AGCAGCTGTC CCCTGATGGC AAAAGCTTCC TGGTATCGGA TCAGTGCCTC 
CAGCATGCCT TCAGCCTGCA CCAGAACATG TGTGCCAACC TGCTGCGGGC ATATCAGAGC 
CTGTTCTGTT ACTTAACCTC CCTGACCAAG AATCTGCCCG CATCCCAGCG CATGGAGCTT 
GCCAAGCCCA GCATGCAGGT CCTTTATGAA CGAGTTCTCA GAAGACCTTA CCCTCCAGGC 
CAAAGGCACG TCTACATAGA GGAACTTGAT GTGGAGGTCC GACTTCATGA GCTTTGTGAA 
CAAGTCAAGC AGAAAGCAGA GAGCCCAGAT GAGTTGGCTG AATTGGTGAA CATGAATTTG 
GCGCAACTAT GTTCCCTACT CATGGCTTTG TGGGGGAATT TCCTGGAAGT TGTGTCACTA 
AATGAGCGTG TTTTTGTGCT TTTGGCCCAG GAGCACCACA CACTACGGGT GCAAAGATTT 
GCTGAAGCTT TCTTTTGTCT TGAACACCCA CGACAGAGTG CACTGGCCTA CCAGGAACTG 
CATGCTCACA GTCACCAACA AATGACCACA GCTATCAAAA GCTCTTCCTA CTTCTTGTCC 
TTACCACCTC TTCCTGTTGA ATGTGCTGAT CTTGACGGGG ATGTGAACTC CCTACCAATC 
ATCTTTGAGG ACAGATATTT GGAGTCTGTT ACTGAAGATC GTGATGGTCC ATGGCTTGGA 
CTCCAAAACA ACAGGAGTGG CTCTGTGTCC GCCAAAGCAG ATAGATCCAA CTCAAAGGAC 
TGCATTGCCA GTGGAGCCCT CGGTCCGTAT CCAGATAAGC AGCAAGCTTC CTTTGCCACT 
GCTTGGCCTG AACAAAACGA GCCTTCCCTT AAATCCAAAG CCAAGTCCAT CAAGCTCAAG 
AAGAACACCA AGACCGAAAA CTCAAAGAAG CTGATCAGAC AGAGCTCGAA AGATTCTGTG 
GTGCTCACAG GCTACAAGAA CTTGAAGGTT CCTCACAGTG AAAAGACCAT TAAGTCCAAA 
GACATCGACC ATCAATGCAA TCAGAGAGAT GAGCTAGAGG ATGGAGGTAA ATTCCTTCTT 
CAGAATGAAA CCACGACCAC GCATTGTGCT CAGAATGCTA AAGACTGCAC ATCTGGCAAC 
CTTCAAGGTA ACGCCACAAT CCTAAGTGAC GGCTTTCCCA ATCTTATGAG TTTGCAAATG 
CAGGCCAGGC CCCAGGCAGG TACTGGAGCA GAGGCCGCCG CTGCCAGAGT AGATCCCACA 
CATCCACCTG CTGGCCTCAG ACATATTGAT GTCAAACCCA GTGACAAGGA CCCCTACCAG 
GGCGATAAGG TCACCGTTCT CTTACCGCAC CACCCAGAGC GCACCACCAA GAGGGATGTT 
CCAGAAATCA CACAGACTGA TTTCTCTGAG TTCAAGAGTC GAGTTGGAGA TGGTGCTACT 
TTCGTCGGAC CTTGTGGAGC GGCTTTGAGG AACGATAATA TTCCTCAAGC CTCCTCCAGA 
CTCTCGGATT CCGGAATTGA AAGCGAACCC AGCTCTGCGA CACAGCTAGC CCCGGTTTCT 
CCATCTCCTT TTGCTCCTAT TGGGATCGAA GCACCTACAG AGCTGATTCC TCACAGACCA 
CCTCCGCTCA GTCCTGCTTC CAGAGGAGCC ACCGCATTGG AAGGCCTCAA TCCTGAAAGC 
ACCAGCGGCG TAAGCGGCGT CCAATCTTCC CTCACCTCCA TCAACTCACT CCCTTCGGAT 
GATGAGGTGG AGACAGAGGC CACCAGCAGG AAGTCCAGCA TCCTGGTCCA GGAACAGAGT 
CTCATCTTCT CCGGAGAATC TCAAAGCCTC TTGCTGACAA ACTCTAGAGT CTTGGCTCAA 
CAAAAAGCCA GTCATGGAGA TGGGATGGAC TCTGCTTTGC AGTCCCGCTG TGAAACTTCT 
GCTTCTGACA GACTTGTCGG ATCTAACGAG ACAGATCTTC CAGGTGGTCC GGAAGCTTGT 
AGTGAACCAC AGACAGCAGC CCAGTCCAGT ACATCAGTCA CCAGCAGCAC AGACATGGTG 
AAGAAAGGCT TTGTAGAAAA CTACTTTGGC TCTCGGTCCA GTACTGATAT ATCTGAAATC 
AGCCCTTTAG AGACGGCAGC CATAACATTG GGTATTCAGA CATGCCCCAA TGCTAATGAT 
GAAGAGGATG AGGAGGAAGA GCAGGAGCAT GAGATGATTG AGAATGGTTA TTATGAGGAG 
ACCGATGGTG TGGTTTATGT TAATGGGCTC CCGGAGGATG AAGGGAGGTG TGAAGATGGA 
GGAGCGGAGG GAAGAACACT GTTTTATGAT CATCTTGGTT TAGAGCAGCT CCAGGAAACT 
TACATTCCCT CTGCCGGCAT TCGGACACGG CCTACTGGAC CAGCACCAAC CAGCAGCGAC 
TTATGCTGGT ACAAGAGCGC CCCCACTGCT CAGATGACGG CTTTTGTCCA AGCGAAAGAG 
GAGTTGAAAC AACTAAAGCT GCCAGGCTTC CTGTACAGTG AGGTGGCAGA TCTGGCATCC 
ACTGTGCCAT ATTTCAGCAT GGATGAGGAT GAAAACTGTG ATGATGGCAT ACACCTTATA 
GTGTGTGTAC ACGGCTTGGA TGGTAACAGT GCAGATTTGC GCCTGGTGAA GACTTATCTG 
GAGCTTGGGT TGCCTGGAGC TAGGATAGAT TTTCTGATGT CTGAGCGAAA TCAGAATGAC 
ACATTTGCTG ATTTTGAGTC CATGACCGAC AGACTTTTGG ATGAAATTGT GCAGTACATA 
CAAATCTACA ACCTCACAGT TTCCAAGATC AGTTTTGTTG GTCATTCTCT GGGAAATCTG 
ATCGTGCGCT CTGTGCTGAC GAGGCCCAGG TTTAAGTGTT ATCTCAGCCG ACTGCACACC 
TTTCTGTCTC TGTCTGGACC TCACCTCGGC ACACTGTACA ACAGCTCAGC ACTCGTCAAT 
ACTGGACTGT GGTTCATGCA GAAGTGGAAG AAGTCTGGCT CTCTGCTGCA GCTGACCTGT 
CGAGACCATT CAGACCCGCG TCAGACCTTT CTCTATAAGC TGAGCAAGAA AGCAGGCCTT 
CAGTTCTTCA AGAATGTGGT GTTGGTGGGC TCTCTTCAGG ACCGATACGT TCCTTATCAC 
TCCGCTCGAA TCGAGATGTG CAAAACAGCC TTAAAGGACA AACAGACTGG TCCGGTGTAT 
GCTGAGATGA TCGAGAACCT TCTCCTTCCT GTGCTTCAGA ACAAAGACTG TAACCTGGTG 
CGTTACGACG TCATCCACGC GCTGCCCAAC ACCGCCAACT CTCTGATCGG CCGCGCAGCT 
CACATCGCCG TCCTGGACTC TGAGATTTTC CTGGAAAAGT TCTTCCTGGT GGCAGGGCTC 
AAGTTTTTTC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam135a ( XM_017358774.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214263.1 Danio rerio family with sequence similarity 135, member A (fam135a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa54720
Clone ID Related Accession (Same CDS sequence)	XM_017358774.1 , XM_017358774.2
Accession Version	XM_017358774.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4035bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein FAM135A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358774.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGGT 
GTTTTCCTTC TGCAGGTAGA AGAGTCTTTA AATGAAGTTG ACTTTCAGCT GTGTTTGGAC 
CTGTTCTTCA CTGATGGAGA CTACTCGCCT GAGGAGCCCA GTTCTCTGCA GAGCATCAGC 
AGCCGTACGC TGCGTTTGCA CTTTAGCCTC CACAGAGGCA TCCACCACCA CGTCAACGTC 
ATGTTTGACT ACTTCCACCT GTCTGTCATC TCTGCCACAA TCCATGCTTC ACTGGTGGCT 
CTGCATCAGC CTCTAATAAG TTTCCCCCGG CCAGTAAAAA GCACCTGGTT GAATCGTAAT 
GCTCCGGCAC AGAGCAAAGA CTCCCTCATC CCACCGCTGG AGACTGTGGT GTTCGGCAGC 
ACCTACGTCA AGCAGCTGTC CCCTGATGGC AAAAGCTTCC TGGTATCGGA TCAGTGCCTC 
CAGCATGCCT TCAGCCTGCA CCAGAACATG TGTGCCAACC TGCTGCGGGC ATATCAGAGC 
CTGTTCTGTT ACTTAACCTC CCTGACCAAG AATCTGCCCG CATCCCAGCG CATGGAGCTT 
GCCAAGCCCA GCATGCAGGT CCTTTATGAA CGAGTTCTCA GAAGACCTTA CCCTCCAGGC 
CAAAGGCACG TCTACATAGA GGAACTTGAT GTGGAGGTCC GACTTCATGA GCTTTGTGAA 
CAAGTCAAGC AGAAAGCAGA GAGCCCAGAT GAGTTGGCTG AATTGGTGAA CATGAATTTG 
GCGCAACTAT GTTCCCTACT CATGGCTTTG TGGGGGAATT TCCTGGAAGT TGTGTCACTA 
AATGAGCGTG TTTTTGTGCT TTTGGCCCAG GAGCACCACA CACTACGGGT GCAAAGATTT 
GCTGAAGCTT TCTTTTGTCT TGAACACCCA CGACAGAGTG CACTGGCCTA CCAGGAACTG 
CATGCTCACA GTCACCAACA AATGACCACA GCTATCAAAA GCTCTTCCTA CTTCTTGTCC 
TTACCACCTC TTCCTGTTGA ATGTGCTGAT CTTGACGGGG ATGTGAACTC CCTACCAATC 
ATCTTTGAGG ACAGATATTT GGAGTCTGTT ACTGAAGATC GTGATGGTCC ATGGCTTGGA 
CTCCAAAACA ACAGGAGTGG CTCTGTGTCC GCCAAAGCAG ATAGATCCAA CTCAAAGGAC 
TGCATTGCCA GTGGAGCCCT CGGTCCGTAT CCAGATAAGC AGCAAGCTTC CTTTGCCACT 
GCTTGGCCTG AACAAAACGA GCCTTCCCTT AAATCCAAAG CCAAGTCCAT CAAGCTCAAG 
AAGAACACCA AGACCGAAAA CTCAAAGAAG CTGATCAGAC AGAGCTCGAA AGATTCTGTG 
GTGCTCACAG GCTACAAGAA CTTGAAGGTT CCTCACAGTG AAAAGACCAT TAAGTCCAAA 
GACATCGACC ATCAATGCAA TCAGAGAGAT GAGCTAGAGG ATGGAGGTAA ATTCCTTCTT 
CAGAATGAAA CCACGACCAC GCATTGTGCT CAGAATGCTA AAGACTGCAC ATCTGGCAAC 
CTTCAAGGTA ACGCCACAAT CCTAAGTGAC GGCTTTCCCA ATCTTATGAG TTTGCAAATG 
CAGGCCAGGC CCCAGGCAGG TACTGGAGCA GAGGCCGCCG CTGCCAGAGT AGATCCCACA 
CATCCACCTG CTGGCCTCAG ACATATTGAT GTCAAACCCA GTGACAAGGA CCCCTACCAG 
GGCGATAAGG TCACCGTTCT CTTACCGCAC CACCCAGAGC GCACCACCAA GAGGGATGTT 
CCAGAAATCA CACAGACTGA TTTCTCTGAG TTCAAGAGTC GAGTTGGAGA TGGTGCTACT 
TTCGTCGGAC CTTGTGGAGC GGCTTTGAGG AACGATAATA TTCCTCAAGC CTCCTCCAGA 
CTCTCGGATT CCGGAATTGA AAGCGAACCC AGCTCTGCGA CACAGCTAGC CCCGGTTTCT 
CCATCTCCTT TTGCTCCTAT TGGGATCGAA GCACCTACAG AGCTGATTCC TCACAGACCA 
CCTCCGCTCA GTCCTGCTTC CAGAGGAGCC ACCGCATTGG AAGGCCTCAA TCCTGAAAGC 
ACCAGCGGCG TAAGCGGCGT CCAATCTTCC CTCACCTCCA TCAACTCACT CCCTTCGGAT 
GATGAGGTGG AGACAGAGGC CACCAGCAGG AAGTCCAGCA TCCTGGTCCA GGAACAGAGT 
CTCATCTTCT CCGGAGAATC TCAAAGCCTC TTGCTGACAA ACTCTAGAGT CTTGGCTCAA 
CAAAAAGCCA GTCATGGAGA TGGGATGGAC TCTGCTTTGC AGTCCCGCTG TGAAACTTCT 
GCTTCTGACA GACTTGTCGG ATCTAACGAG ACAGATCTTC CAGGTGGTCC GGAAGCTTGT 
AGTGAACCAC AGACAGCAGC CCAGTCCAGT ACATCAGTCA CCAGCAGCAC AGACATGGTG 
AAGAAAGGCT TTGTAGAAAA CTACTTTGGC TCTCGGTCCA GTACTGATAT ATCTGAAATC 
AGCCCTTTAG AGACGGCAGC CATAACATTG GGTATTCAGA CATGCCCCAA TGCTAATGAT 
GAAGAGGATG AGGAGGAAGA GCAGGAGCAT GAGATGATTG AGAATGGTTA TTATGAGGAG 
ACCGATGGTG TGGTTTATGT TAATGGGCTC CCGGAGGATG AAGGGAGGTG TGAAGATGGA 
GGAGCGGAGG GAAGAACACT GTTTTATGAT CATCTTGGTT TAGAGCAGCT CCAGGAAACT 
TACATTCCCT CTGCCGGCAT TCGGACACGG CCTACTGGAC CAGCACCAAC CAGCAGCGAC 
TTATGCTGGT ACAAGAGCGC CCCCACTGCT CAGATGACGG CTTTTGTCCA AGCGAAAGAG 
GAGTTGAAAC AACTAAAGCT GCCAGGCTTC CTGTACAGTG AGGTGGCAGA TCTGGCATCC 
ACTGTGCCAT ATTTCAGCAT GGATGAGGAT GAAAACTGTG ATGATGGCAT ACACCTTATA 
GTGTGTGTAC ACGGCTTGGA TGGTAACAGT GCAGATTTGC GCCTGGTGAA GACTTATCTG 
GAGCTTGGGT TGCCTGGAGC TAGGATAGAT TTTCTGATGT CTGAGCGAAA TCAGAATGAC 
ACATTTGCTG ATTTTGAGTC CATGACCGAC AGACTTTTGG ATGAAATTGT GCAGTACATA 
CAAATCTACA ACCTCACAGT TTCCAAGATC AGTTTTGTTG GTCATTCTCT GGGAAATCTG 
ATCGTGCGCT CTGTGCTGAC GAGGCCCAGG TTTAAGTGTT ATCTCAGCCG ACTGCACACC 
TTTCTGTCTC TGTCTGGACC TCACCTCGGC ACACTGTACA ACAGCTCAGC ACTCGTCAAT 
ACTGGACTGT GGTTCATGCA GAAGTGGAAG AAGTCTGGCT CTCTGCTGCA GCTGACCTGT 
CGAGACCATT CAGACCCGCG TCAGACCTTT CTCTATAAGC TGAGCAAGAA AGCAGGCCTT 
CAGTTCTTCA AGAATGTGGT GTTGGTGGGC TCTCTTCAGG ACCGATACGT TCCTTATCAC 
TCCGCTCGAA TCGAGATGTG CAAAACAGCC TTAAAGGACA AACAGACTGG TCCGGTGTAT 
GCTGAGATGA TCGAGAACCT TCTCCTTCCT GTGCTTCAGA ACAAAGACTG TAACCTGGTG 
CGTTACGACG TCATCCACGC GCTGCCCAAC ACCGCCAACT CTCTGATCGG CCGCGCAGCT 
CACATCGCCG TCCTGGACTC TGAGATTTTC CTGGAAAAGT TCTTCCTGGT GGCAGGGCTC 
AAGTTTTTTC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214263.1
CDS	405..4439
Translation	MTEVQATVEFSVELHKFYNVDLFQRGFYQVRASMKVPPRIPQRVEASLLHPIGSDLAFPASVQDDVVCSKTFQILYKNEEIVVNDVFVFKVMMLLDAKKGVFLLQVEESLNEVDFQLCLDLFFTDGDYSPEEPSSLQSISSRTLRLHFSLHRGIHHHVNVMFDYFHLSVISATIHASLVALHQPLISFPRPVKSTWLNRNAPAQSKDSLIPPLETVVFGSTYVKQLSPDGKSFLVSDQCLQHAFSLHQNMCANLLRAYQSLFCYLTSLTKNLPASQRMELAKPSMQVLYERVLRRPYPPGQRHVYIEELDVEVRLHELCEQVKQKAESPDELAELVNMNLAQLCSLLMALWGNFLEVVSLNERVFVLLAQEHHTLRVQRFAEAFFCLEHPRQSALAYQELHAHSHQQMTTAIKSSSYFLSLPPLPVECADLDGDVNSLPIIFEDRYLESVTEDRDGPWLGLQNNRSGSVSAKADRSNSKDCIASGALGPYPDKQQASFATAWPEQNEPSLKSKAKSIKLKKNTKTENSKKLIRQSSKDSVVLTGYKNLKVPHSEKTIKSKDIDHQCNQRDELEDGGKFLLQNETTTTHCAQNAKDCTSGNLQGNATILSDGFPNLMSLQMQARPQAGTGAEAAAARVDPTHPPAGLRHIDVKPSDKDPYQGDKVTVLLPHHPERTTKRDVPEITQTDFSEFKSRVGDGATFVGPCGAALRNDNIPQASSRLSDSGIESEPSSATQLAPVSPSPFAPIGIEAPTELIPHRPPPLSPASRGATALEGLNPESTSGVSGVQSSLTSINSLPSDDEVETEATSRKSSILVQEQSLIFSGESQSLLLTNSRVLAQQKASHGDGMDSALQSRCETSASDRLVGSNETDLPGGPEACSEPQTAAQSSTSVTSSTDMVKKGFVENYFGSRSSTDISEISPLETAAITLGIQTCPNANDEEDEEEEQEHEMIENGYYEETDGVVYVNGLPEDEGRCEDGGAEGRTLFYDHLGLEQLQETYIPSAGIRTRPTGPAPTSSDLCWYKSAPTAQMTAFVQAKEELKQLKLPGFLYSEVADLASTVPYFSMDEDENCDDGIHLIVCVHGLDGNSADLRLVKTYLELGLPGARIDFLMSERNQNDTFADFESMTDRLLDEIVQYIQIYNLTVSKISFVGHSLGNLIVRSVLTRPRFKCYLSRLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSKKAGLQFFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQTGPVYAEMIENLLLPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEIFLEKFFLVAGLKFFQ

Target ORF information:

RefSeq Version	XM_017358774.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio family with sequence similarity 135, member A (fam135a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358774.2

ORF Insert Sequence:

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGGT 
GTTTTCCTTC TGCAGGTAGA AGAGTCTTTA AATGAAGTTG ACTTTCAGCT GTGTTTGGAC 
CTGTTCTTCA CTGATGGAGA CTACTCGCCT GAGGAGCCCA GTTCTCTGCA GAGCATCAGC 
AGCCGTACGC TGCGTTTGCA CTTTAGCCTC CACAGAGGCA TCCACCACCA CGTCAACGTC 
ATGTTTGACT ACTTCCACCT GTCTGTCATC TCTGCCACAA TCCATGCTTC ACTGGTGGCT 
CTGCATCAGC CTCTAATAAG TTTCCCCCGG CCAGTAAAAA GCACCTGGTT GAATCGTAAT 
GCTCCGGCAC AGAGCAAAGA CTCCCTCATC CCACCGCTGG AGACTGTGGT GTTCGGCAGC 
ACCTACGTCA AGCAGCTGTC CCCTGATGGC AAAAGCTTCC TGGTATCGGA TCAGTGCCTC 
CAGCATGCCT TCAGCCTGCA CCAGAACATG TGTGCCAACC TGCTGCGGGC ATATCAGAGC 
CTGTTCTGTT ACTTAACCTC CCTGACCAAG AATCTGCCCG CATCCCAGCG CATGGAGCTT 
GCCAAGCCCA GCATGCAGGT CCTTTATGAA CGAGTTCTCA GAAGACCTTA CCCTCCAGGC 
CAAAGGCACG TCTACATAGA GGAACTTGAT GTGGAGGTCC GACTTCATGA GCTTTGTGAA 
CAAGTCAAGC AGAAAGCAGA GAGCCCAGAT GAGTTGGCTG AATTGGTGAA CATGAATTTG 
GCGCAACTAT GTTCCCTACT CATGGCTTTG TGGGGGAATT TCCTGGAAGT TGTGTCACTA 
AATGAGCGTG TTTTTGTGCT TTTGGCCCAG GAGCACCACA CACTACGGGT GCAAAGATTT 
GCTGAAGCTT TCTTTTGTCT TGAACACCCA CGACAGAGTG CACTGGCCTA CCAGGAACTG 
CATGCTCACA GTCACCAACA AATGACCACA GCTATCAAAA GCTCTTCCTA CTTCTTGTCC 
TTACCACCTC TTCCTGTTGA ATGTGCTGAT CTTGACGGGG ATGTGAACTC CCTACCAATC 
ATCTTTGAGG ACAGATATTT GGAGTCTGTT ACTGAAGATC GTGATGGTCC ATGGCTTGGA 
CTCCAAAACA ACAGGAGTGG CTCTGTGTCC GCCAAAGCAG ATAGATCCAA CTCAAAGGAC 
TGCATTGCCA GTGGAGCCCT CGGTCCGTAT CCAGATAAGC AGCAAGCTTC CTTTGCCACT 
GCTTGGCCTG AACAAAACGA GCCTTCCCTT AAATCCAAAG CCAAGTCCAT CAAGCTCAAG 
AAGAACACCA AGACCGAAAA CTCAAAGAAG CTGATCAGAC AGAGCTCGAA AGATTCTGTG 
GTGCTCACAG GCTACAAGAA CTTGAAGGTT CCTCACAGTG AAAAGACCAT TAAGTCCAAA 
GACATCGACC ATCAATGCAA TCAGAGAGAT GAGCTAGAGG ATGGAGGTAA ATTCCTTCTT 
CAGAATGAAA CCACGACCAC GCATTGTGCT CAGAATGCTA AAGACTGCAC ATCTGGCAAC 
CTTCAAGGTA ACGCCACAAT CCTAAGTGAC GGCTTTCCCA ATCTTATGAG TTTGCAAATG 
CAGGCCAGGC CCCAGGCAGG TACTGGAGCA GAGGCCGCCG CTGCCAGAGT AGATCCCACA 
CATCCACCTG CTGGCCTCAG ACATATTGAT GTCAAACCCA GTGACAAGGA CCCCTACCAG 
GGCGATAAGG TCACCGTTCT CTTACCGCAC CACCCAGAGC GCACCACCAA GAGGGATGTT 
CCAGAAATCA CACAGACTGA TTTCTCTGAG TTCAAGAGTC GAGTTGGAGA TGGTGCTACT 
TTCGTCGGAC CTTGTGGAGC GGCTTTGAGG AACGATAATA TTCCTCAAGC CTCCTCCAGA 
CTCTCGGATT CCGGAATTGA AAGCGAACCC AGCTCTGCGA CACAGCTAGC CCCGGTTTCT 
CCATCTCCTT TTGCTCCTAT TGGGATCGAA GCACCTACAG AGCTGATTCC TCACAGACCA 
CCTCCGCTCA GTCCTGCTTC CAGAGGAGCC ACCGCATTGG AAGGCCTCAA TCCTGAAAGC 
ACCAGCGGCG TAAGCGGCGT CCAATCTTCC CTCACCTCCA TCAACTCACT CCCTTCGGAT 
GATGAGGTGG AGACAGAGGC CACCAGCAGG AAGTCCAGCA TCCTGGTCCA GGAACAGAGT 
CTCATCTTCT CCGGAGAATC TCAAAGCCTC TTGCTGACAA ACTCTAGAGT CTTGGCTCAA 
CAAAAAGCCA GTCATGGAGA TGGGATGGAC TCTGCTTTGC AGTCCCGCTG TGAAACTTCT 
GCTTCTGACA GACTTGTCGG ATCTAACGAG ACAGATCTTC CAGGTGGTCC GGAAGCTTGT 
AGTGAACCAC AGACAGCAGC CCAGTCCAGT ACATCAGTCA CCAGCAGCAC AGACATGGTG 
AAGAAAGGCT TTGTAGAAAA CTACTTTGGC TCTCGGTCCA GTACTGATAT ATCTGAAATC 
AGCCCTTTAG AGACGGCAGC CATAACATTG GGTATTCAGA CATGCCCCAA TGCTAATGAT 
GAAGAGGATG AGGAGGAAGA GCAGGAGCAT GAGATGATTG AGAATGGTTA TTATGAGGAG 
ACCGATGGTG TGGTTTATGT TAATGGGCTC CCGGAGGATG AAGGGAGGTG TGAAGATGGA 
GGAGCGGAGG GAAGAACACT GTTTTATGAT CATCTTGGTT TAGAGCAGCT CCAGGAAACT 
TACATTCCCT CTGCCGGCAT TCGGACACGG CCTACTGGAC CAGCACCAAC CAGCAGCGAC 
TTATGCTGGT ACAAGAGCGC CCCCACTGCT CAGATGACGG CTTTTGTCCA AGCGAAAGAG 
GAGTTGAAAC AACTAAAGCT GCCAGGCTTC CTGTACAGTG AGGTGGCAGA TCTGGCATCC 
ACTGTGCCAT ATTTCAGCAT GGATGAGGAT GAAAACTGTG ATGATGGCAT ACACCTTATA 
GTGTGTGTAC ACGGCTTGGA TGGTAACAGT GCAGATTTGC GCCTGGTGAA GACTTATCTG 
GAGCTTGGGT TGCCTGGAGC TAGGATAGAT TTTCTGATGT CTGAGCGAAA TCAGAATGAC 
ACATTTGCTG ATTTTGAGTC CATGACCGAC AGACTTTTGG ATGAAATTGT GCAGTACATA 
CAAATCTACA ACCTCACAGT TTCCAAGATC AGTTTTGTTG GTCATTCTCT GGGAAATCTG 
ATCGTGCGCT CTGTGCTGAC GAGGCCCAGG TTTAAGTGTT ATCTCAGCCG ACTGCACACC 
TTTCTGTCTC TGTCTGGACC TCACCTCGGC ACACTGTACA ACAGCTCAGC ACTCGTCAAT 
ACTGGACTGT GGTTCATGCA GAAGTGGAAG AAGTCTGGCT CTCTGCTGCA GCTGACCTGT 
CGAGACCATT CAGACCCGCG TCAGACCTTT CTCTATAAGC TGAGCAAGAA AGCAGGCCTT 
CAGTTCTTCA AGAATGTGGT GTTGGTGGGC TCTCTTCAGG ACCGATACGT TCCTTATCAC 
TCCGCTCGAA TCGAGATGTG CAAAACAGCC TTAAAGGACA AACAGACTGG TCCGGTGTAT 
GCTGAGATGA TCGAGAACCT TCTCCTTCCT GTGCTTCAGA ACAAAGACTG TAACCTGGTG 
CGTTACGACG TCATCCACGC GCTGCCCAAC ACCGCCAACT CTCTGATCGG CCGCGCAGCT 
CACATCGCCG TCCTGGACTC TGAGATTTTC CTGGAAAAGT TCTTCCTGGT GGCAGGGCTC 
AAGTTTTTTC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam135a ( XM_017358775.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214264.1 Danio rerio family with sequence similarity 135, member A (fam135a), transcript variant X2, mRNA.

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CloneID	ODa54721
Clone ID Related Accession (Same CDS sequence)	XM_017358775.2 , XM_017358775.1
Accession Version	XM_017358775.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein FAM135A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358775.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGGT 
GTTTTCCTTC TGCAGGTAGA AGAGTCTTTA AATGAAGTTG ACTTTCAGCT GTGTTTGGAC 
CTGTTCTTCA CTGATGGAGA CTACTCGCCT GAGGAGCCCA GTTCTCTGCA GAGCATCAGC 
AGCCGTACGC TGCGTTTGCA CTTTAGCCTC CACAGAGGCA TCCACCACCA CGTCAACGTC 
ATGTTTGACT ACTTCCACCT GTCTGTCATC TCTGCCACAA TCCATGCTTC ACTGGTGGCT 
CTGCATCAGC CTCTAATAAG TTTCCCCCGG CCAGTAAAAA GCACCTGGTT GAATCGTAAT 
GCTCCGGCAC AGAGCAAAGA CTCCCTCATC CCACCGCTGG AGACTGTGGT GTTCGGCAGC 
ACCTACGTCA AGCAGCTGTC CCCTGATGGC AAAAGCTTCC TGGTATCGGA TCAGTGCCTC 
CAGCATGCCT TCAGCCTGCA CCAGAACATG TGTGCCAACC TGCTGCGGGC ATATCAGAGC 
CTGTTCTGTT ACTTAACCTC CCTGACCAAG AATCTGCCCG CATCCCAGCG CATGGAGCTT 
GCCAAGCCCA GCATGCAGGT CCTTTATGAA CGAGTTCTCA GAAGACCTTA CCCTCCAGGC 
CAAAGGCACG TCTACATAGA GGAACTTGAT GTGGAGGTCC GACTTCATGA GCTTTGTGAA 
CAAGTCAAGA AAGCAGAGAG CCCAGATGAG TTGGCTGAAT TGGTGAACAT GAATTTGGCG 
CAACTATGTT CCCTACTCAT GGCTTTGTGG GGGAATTTCC TGGAAGTTGT GTCACTAAAT 
GAGCGTGTTT TTGTGCTTTT GGCCCAGGAG CACCACACAC TACGGGTGCA AAGATTTGCT 
GAAGCTTTCT TTTGTCTTGA ACACCCACGA CAGAGTGCAC TGGCCTACCA GGAACTGCAT 
GCTCACAGTC ACCAACAAAT GACCACAGCT ATCAAAAGCT CTTCCTACTT CTTGTCCTTA 
CCACCTCTTC CTGTTGAATG TGCTGATCTT GACGGGGATG TGAACTCCCT ACCAATCATC 
TTTGAGGACA GATATTTGGA GTCTGTTACT GAAGATCGTG ATGGTCCATG GCTTGGACTC 
CAAAACAACA GGAGTGGCTC TGTGTCCGCC AAAGCAGATA GATCCAACTC AAAGGACTGC 
ATTGCCAGTG GAGCCCTCGG TCCGTATCCA GATAAGCAGC AAGCTTCCTT TGCCACTGCT 
TGGCCTGAAC AAAACGAGCC TTCCCTTAAA TCCAAAGCCA AGTCCATCAA GCTCAAGAAG 
AACACCAAGA CCGAAAACTC AAAGAAGCTG ATCAGACAGA GCTCGAAAGA TTCTGTGGTG 
CTCACAGGCT ACAAGAACTT GAAGGTTCCT CACAGTGAAA AGACCATTAA GTCCAAAGAC 
ATCGACCATC AATGCAATCA GAGAGATGAG CTAGAGGATG GAGGTAAATT CCTTCTTCAG 
AATGAAACCA CGACCACGCA TTGTGCTCAG AATGCTAAAG ACTGCACATC TGGCAACCTT 
CAAGGTAACG CCACAATCCT AAGTGACGGC TTTCCCAATC TTATGAGTTT GCAAATGCAG 
GCCAGGCCCC AGGCAGGTAC TGGAGCAGAG GCCGCCGCTG CCAGAGTAGA TCCCACACAT 
CCACCTGCTG GCCTCAGACA TATTGATGTC AAACCCAGTG ACAAGGACCC CTACCAGGGC 
GATAAGGTCA CCGTTCTCTT ACCGCACCAC CCAGAGCGCA CCACCAAGAG GGATGTTCCA 
GAAATCACAC AGACTGATTT CTCTGAGTTC AAGAGTCGAG TTGGAGATGG TGCTACTTTC 
GTCGGACCTT GTGGAGCGGC TTTGAGGAAC GATAATATTC CTCAAGCCTC CTCCAGACTC 
TCGGATTCCG GAATTGAAAG CGAACCCAGC TCTGCGACAC AGCTAGCCCC GGTTTCTCCA 
TCTCCTTTTG CTCCTATTGG GATCGAAGCA CCTACAGAGC TGATTCCTCA CAGACCACCT 
CCGCTCAGTC CTGCTTCCAG AGGAGCCACC GCATTGGAAG GCCTCAATCC TGAAAGCACC 
AGCGGCGTAA GCGGCGTCCA ATCTTCCCTC ACCTCCATCA ACTCACTCCC TTCGGATGAT 
GAGGTGGAGA CAGAGGCCAC CAGCAGGAAG TCCAGCATCC TGGTCCAGGA ACAGAGTCTC 
ATCTTCTCCG GAGAATCTCA AAGCCTCTTG CTGACAAACT CTAGAGTCTT GGCTCAACAA 
AAAGCCAGTC ATGGAGATGG GATGGACTCT GCTTTGCAGT CCCGCTGTGA AACTTCTGCT 
TCTGACAGAC TTGTCGGATC TAACGAGACA GATCTTCCAG GTGGTCCGGA AGCTTGTAGT 
GAACCACAGA CAGCAGCCCA GTCCAGTACA TCAGTCACCA GCAGCACAGA CATGGTGAAG 
AAAGGCTTTG TAGAAAACTA CTTTGGCTCT CGGTCCAGTA CTGATATATC TGAAATCAGC 
CCTTTAGAGA CGGCAGCCAT AACATTGGGT ATTCAGACAT GCCCCAATGC TAATGATGAA 
GAGGATGAGG AGGAAGAGCA GGAGCATGAG ATGATTGAGA ATGGTTATTA TGAGGAGACC 
GATGGTGTGG TTTATGTTAA TGGGCTCCCG GAGGATGAAG GGAGGTGTGA AGATGGAGGA 
GCGGAGGGAA GAACACTGTT TTATGATCAT CTTGGTTTAG AGCAGCTCCA GGAAACTTAC 
ATTCCCTCTG CCGGCATTCG GACACGGCCT ACTGGACCAG CACCAACCAG CAGCGACTTA 
TGCTGGTACA AGAGCGCCCC CACTGCTCAG ATGACGGCTT TTGTCCAAGC GAAAGAGGAG 
TTGAAACAAC TAAAGCTGCC AGGCTTCCTG TACAGTGAGG TGGCAGATCT GGCATCCACT 
GTGCCATATT TCAGCATGGA TGAGGATGAA AACTGTGATG ATGGCATACA CCTTATAGTG 
TGTGTACACG GCTTGGATGG TAACAGTGCA GATTTGCGCC TGGTGAAGAC TTATCTGGAG 
CTTGGGTTGC CTGGAGCTAG GATAGATTTT CTGATGTCTG AGCGAAATCA GAATGACACA 
TTTGCTGATT TTGAGTCCAT GACCGACAGA CTTTTGGATG AAATTGTGCA GTACATACAA 
ATCTACAACC TCACAGTTTC CAAGATCAGT TTTGTTGGTC ATTCTCTGGG AAATCTGATC 
GTGCGCTCTG TGCTGACGAG GCCCAGGTTT AAGTGTTATC TCAGCCGACT GCACACCTTT 
CTGTCTCTGT CTGGACCTCA CCTCGGCACA CTGTACAACA GCTCAGCACT CGTCAATACT 
GGACTGTGGT TCATGCAGAA GTGGAAGAAG TCTGGCTCTC TGCTGCAGCT GACCTGTCGA 
GACCATTCAG ACCCGCGTCA GACCTTTCTC TATAAGCTGA GCAAGAAAGC AGGCCTTCAG 
TTCTTCAAGA ATGTGGTGTT GGTGGGCTCT CTTCAGGACC GATACGTTCC TTATCACTCC 
GCTCGAATCG AGATGTGCAA AACAGCCTTA AAGGACAAAC AGACTGGTCC GGTGTATGCT 
GAGATGATCG AGAACCTTCT CCTTCCTGTG CTTCAGAACA AAGACTGTAA CCTGGTGCGT 
TACGACGTCA TCCACGCGCT GCCCAACACC GCCAACTCTC TGATCGGCCG CGCAGCTCAC 
ATCGCCGTCC TGGACTCTGA GATTTTCCTG GAAAAGTTCT TCCTGGTGGC AGGGCTCAAG 
TTTTTTCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214264.1
CDS	405..4436
Translation	MTEVQATVEFSVELHKFYNVDLFQRGFYQVRASMKVPPRIPQRVEASLLHPIGSDLAFPASVQDDVVCSKTFQILYKNEEIVVNDVFVFKVMMLLDAKKGVFLLQVEESLNEVDFQLCLDLFFTDGDYSPEEPSSLQSISSRTLRLHFSLHRGIHHHVNVMFDYFHLSVISATIHASLVALHQPLISFPRPVKSTWLNRNAPAQSKDSLIPPLETVVFGSTYVKQLSPDGKSFLVSDQCLQHAFSLHQNMCANLLRAYQSLFCYLTSLTKNLPASQRMELAKPSMQVLYERVLRRPYPPGQRHVYIEELDVEVRLHELCEQVKKAESPDELAELVNMNLAQLCSLLMALWGNFLEVVSLNERVFVLLAQEHHTLRVQRFAEAFFCLEHPRQSALAYQELHAHSHQQMTTAIKSSSYFLSLPPLPVECADLDGDVNSLPIIFEDRYLESVTEDRDGPWLGLQNNRSGSVSAKADRSNSKDCIASGALGPYPDKQQASFATAWPEQNEPSLKSKAKSIKLKKNTKTENSKKLIRQSSKDSVVLTGYKNLKVPHSEKTIKSKDIDHQCNQRDELEDGGKFLLQNETTTTHCAQNAKDCTSGNLQGNATILSDGFPNLMSLQMQARPQAGTGAEAAAARVDPTHPPAGLRHIDVKPSDKDPYQGDKVTVLLPHHPERTTKRDVPEITQTDFSEFKSRVGDGATFVGPCGAALRNDNIPQASSRLSDSGIESEPSSATQLAPVSPSPFAPIGIEAPTELIPHRPPPLSPASRGATALEGLNPESTSGVSGVQSSLTSINSLPSDDEVETEATSRKSSILVQEQSLIFSGESQSLLLTNSRVLAQQKASHGDGMDSALQSRCETSASDRLVGSNETDLPGGPEACSEPQTAAQSSTSVTSSTDMVKKGFVENYFGSRSSTDISEISPLETAAITLGIQTCPNANDEEDEEEEQEHEMIENGYYEETDGVVYVNGLPEDEGRCEDGGAEGRTLFYDHLGLEQLQETYIPSAGIRTRPTGPAPTSSDLCWYKSAPTAQMTAFVQAKEELKQLKLPGFLYSEVADLASTVPYFSMDEDENCDDGIHLIVCVHGLDGNSADLRLVKTYLELGLPGARIDFLMSERNQNDTFADFESMTDRLLDEIVQYIQIYNLTVSKISFVGHSLGNLIVRSVLTRPRFKCYLSRLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSKKAGLQFFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQTGPVYAEMIENLLLPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEIFLEKFFLVAGLKFFQ

Target ORF information:

RefSeq Version	XM_017358775.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio family with sequence similarity 135, member A (fam135a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358775.2

ORF Insert Sequence:

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGGT 
GTTTTCCTTC TGCAGGTAGA AGAGTCTTTA AATGAAGTTG ACTTTCAGCT GTGTTTGGAC 
CTGTTCTTCA CTGATGGAGA CTACTCGCCT GAGGAGCCCA GTTCTCTGCA GAGCATCAGC 
AGCCGTACGC TGCGTTTGCA CTTTAGCCTC CACAGAGGCA TCCACCACCA CGTCAACGTC 
ATGTTTGACT ACTTCCACCT GTCTGTCATC TCTGCCACAA TCCATGCTTC ACTGGTGGCT 
CTGCATCAGC CTCTAATAAG TTTCCCCCGG CCAGTAAAAA GCACCTGGTT GAATCGTAAT 
GCTCCGGCAC AGAGCAAAGA CTCCCTCATC CCACCGCTGG AGACTGTGGT GTTCGGCAGC 
ACCTACGTCA AGCAGCTGTC CCCTGATGGC AAAAGCTTCC TGGTATCGGA TCAGTGCCTC 
CAGCATGCCT TCAGCCTGCA CCAGAACATG TGTGCCAACC TGCTGCGGGC ATATCAGAGC 
CTGTTCTGTT ACTTAACCTC CCTGACCAAG AATCTGCCCG CATCCCAGCG CATGGAGCTT 
GCCAAGCCCA GCATGCAGGT CCTTTATGAA CGAGTTCTCA GAAGACCTTA CCCTCCAGGC 
CAAAGGCACG TCTACATAGA GGAACTTGAT GTGGAGGTCC GACTTCATGA GCTTTGTGAA 
CAAGTCAAGA AAGCAGAGAG CCCAGATGAG TTGGCTGAAT TGGTGAACAT GAATTTGGCG 
CAACTATGTT CCCTACTCAT GGCTTTGTGG GGGAATTTCC TGGAAGTTGT GTCACTAAAT 
GAGCGTGTTT TTGTGCTTTT GGCCCAGGAG CACCACACAC TACGGGTGCA AAGATTTGCT 
GAAGCTTTCT TTTGTCTTGA ACACCCACGA CAGAGTGCAC TGGCCTACCA GGAACTGCAT 
GCTCACAGTC ACCAACAAAT GACCACAGCT ATCAAAAGCT CTTCCTACTT CTTGTCCTTA 
CCACCTCTTC CTGTTGAATG TGCTGATCTT GACGGGGATG TGAACTCCCT ACCAATCATC 
TTTGAGGACA GATATTTGGA GTCTGTTACT GAAGATCGTG ATGGTCCATG GCTTGGACTC 
CAAAACAACA GGAGTGGCTC TGTGTCCGCC AAAGCAGATA GATCCAACTC AAAGGACTGC 
ATTGCCAGTG GAGCCCTCGG TCCGTATCCA GATAAGCAGC AAGCTTCCTT TGCCACTGCT 
TGGCCTGAAC AAAACGAGCC TTCCCTTAAA TCCAAAGCCA AGTCCATCAA GCTCAAGAAG 
AACACCAAGA CCGAAAACTC AAAGAAGCTG ATCAGACAGA GCTCGAAAGA TTCTGTGGTG 
CTCACAGGCT ACAAGAACTT GAAGGTTCCT CACAGTGAAA AGACCATTAA GTCCAAAGAC 
ATCGACCATC AATGCAATCA GAGAGATGAG CTAGAGGATG GAGGTAAATT CCTTCTTCAG 
AATGAAACCA CGACCACGCA TTGTGCTCAG AATGCTAAAG ACTGCACATC TGGCAACCTT 
CAAGGTAACG CCACAATCCT AAGTGACGGC TTTCCCAATC TTATGAGTTT GCAAATGCAG 
GCCAGGCCCC AGGCAGGTAC TGGAGCAGAG GCCGCCGCTG CCAGAGTAGA TCCCACACAT 
CCACCTGCTG GCCTCAGACA TATTGATGTC AAACCCAGTG ACAAGGACCC CTACCAGGGC 
GATAAGGTCA CCGTTCTCTT ACCGCACCAC CCAGAGCGCA CCACCAAGAG GGATGTTCCA 
GAAATCACAC AGACTGATTT CTCTGAGTTC AAGAGTCGAG TTGGAGATGG TGCTACTTTC 
GTCGGACCTT GTGGAGCGGC TTTGAGGAAC GATAATATTC CTCAAGCCTC CTCCAGACTC 
TCGGATTCCG GAATTGAAAG CGAACCCAGC TCTGCGACAC AGCTAGCCCC GGTTTCTCCA 
TCTCCTTTTG CTCCTATTGG GATCGAAGCA CCTACAGAGC TGATTCCTCA CAGACCACCT 
CCGCTCAGTC CTGCTTCCAG AGGAGCCACC GCATTGGAAG GCCTCAATCC TGAAAGCACC 
AGCGGCGTAA GCGGCGTCCA ATCTTCCCTC ACCTCCATCA ACTCACTCCC TTCGGATGAT 
GAGGTGGAGA CAGAGGCCAC CAGCAGGAAG TCCAGCATCC TGGTCCAGGA ACAGAGTCTC 
ATCTTCTCCG GAGAATCTCA AAGCCTCTTG CTGACAAACT CTAGAGTCTT GGCTCAACAA 
AAAGCCAGTC ATGGAGATGG GATGGACTCT GCTTTGCAGT CCCGCTGTGA AACTTCTGCT 
TCTGACAGAC TTGTCGGATC TAACGAGACA GATCTTCCAG GTGGTCCGGA AGCTTGTAGT 
GAACCACAGA CAGCAGCCCA GTCCAGTACA TCAGTCACCA GCAGCACAGA CATGGTGAAG 
AAAGGCTTTG TAGAAAACTA CTTTGGCTCT CGGTCCAGTA CTGATATATC TGAAATCAGC 
CCTTTAGAGA CGGCAGCCAT AACATTGGGT ATTCAGACAT GCCCCAATGC TAATGATGAA 
GAGGATGAGG AGGAAGAGCA GGAGCATGAG ATGATTGAGA ATGGTTATTA TGAGGAGACC 
GATGGTGTGG TTTATGTTAA TGGGCTCCCG GAGGATGAAG GGAGGTGTGA AGATGGAGGA 
GCGGAGGGAA GAACACTGTT TTATGATCAT CTTGGTTTAG AGCAGCTCCA GGAAACTTAC 
ATTCCCTCTG CCGGCATTCG GACACGGCCT ACTGGACCAG CACCAACCAG CAGCGACTTA 
TGCTGGTACA AGAGCGCCCC CACTGCTCAG ATGACGGCTT TTGTCCAAGC GAAAGAGGAG 
TTGAAACAAC TAAAGCTGCC AGGCTTCCTG TACAGTGAGG TGGCAGATCT GGCATCCACT 
GTGCCATATT TCAGCATGGA TGAGGATGAA AACTGTGATG ATGGCATACA CCTTATAGTG 
TGTGTACACG GCTTGGATGG TAACAGTGCA GATTTGCGCC TGGTGAAGAC TTATCTGGAG 
CTTGGGTTGC CTGGAGCTAG GATAGATTTT CTGATGTCTG AGCGAAATCA GAATGACACA 
TTTGCTGATT TTGAGTCCAT GACCGACAGA CTTTTGGATG AAATTGTGCA GTACATACAA 
ATCTACAACC TCACAGTTTC CAAGATCAGT TTTGTTGGTC ATTCTCTGGG AAATCTGATC 
GTGCGCTCTG TGCTGACGAG GCCCAGGTTT AAGTGTTATC TCAGCCGACT GCACACCTTT 
CTGTCTCTGT CTGGACCTCA CCTCGGCACA CTGTACAACA GCTCAGCACT CGTCAATACT 
GGACTGTGGT TCATGCAGAA GTGGAAGAAG TCTGGCTCTC TGCTGCAGCT GACCTGTCGA 
GACCATTCAG ACCCGCGTCA GACCTTTCTC TATAAGCTGA GCAAGAAAGC AGGCCTTCAG 
TTCTTCAAGA ATGTGGTGTT GGTGGGCTCT CTTCAGGACC GATACGTTCC TTATCACTCC 
GCTCGAATCG AGATGTGCAA AACAGCCTTA AAGGACAAAC AGACTGGTCC GGTGTATGCT 
GAGATGATCG AGAACCTTCT CCTTCCTGTG CTTCAGAACA AAGACTGTAA CCTGGTGCGT 
TACGACGTCA TCCACGCGCT GCCCAACACC GCCAACTCTC TGATCGGCCG CGCAGCTCAC 
ATCGCCGTCC TGGACTCTGA GATTTTCCTG GAAAAGTTCT TCCTGGTGGC AGGGCTCAAG 
TTTTTTCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam135a ( XM_017358775.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214264.1 Danio rerio family with sequence similarity 135 member A (fam135a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa54721
Clone ID Related Accession (Same CDS sequence)	XM_017358775.2 , XM_017358775.1
Accession Version	XM_017358775.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein FAM135A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGGT 
GTTTTCCTTC TGCAGGTAGA AGAGTCTTTA AATGAAGTTG ACTTTCAGCT GTGTTTGGAC 
CTGTTCTTCA CTGATGGAGA CTACTCGCCT GAGGAGCCCA GTTCTCTGCA GAGCATCAGC 
AGCCGTACGC TGCGTTTGCA CTTTAGCCTC CACAGAGGCA TCCACCACCA CGTCAACGTC 
ATGTTTGACT ACTTCCACCT GTCTGTCATC TCTGCCACAA TCCATGCTTC ACTGGTGGCT 
CTGCATCAGC CTCTAATAAG TTTCCCCCGG CCAGTAAAAA GCACCTGGTT GAATCGTAAT 
GCTCCGGCAC AGAGCAAAGA CTCCCTCATC CCACCGCTGG AGACTGTGGT GTTCGGCAGC 
ACCTACGTCA AGCAGCTGTC CCCTGATGGC AAAAGCTTCC TGGTATCGGA TCAGTGCCTC 
CAGCATGCCT TCAGCCTGCA CCAGAACATG TGTGCCAACC TGCTGCGGGC ATATCAGAGC 
CTGTTCTGTT ACTTAACCTC CCTGACCAAG AATCTGCCCG CATCCCAGCG CATGGAGCTT 
GCCAAGCCCA GCATGCAGGT CCTTTATGAA CGAGTTCTCA GAAGACCTTA CCCTCCAGGC 
CAAAGGCACG TCTACATAGA GGAACTTGAT GTGGAGGTCC GACTTCATGA GCTTTGTGAA 
CAAGTCAAGA AAGCAGAGAG CCCAGATGAG TTGGCTGAAT TGGTGAACAT GAATTTGGCG 
CAACTATGTT CCCTACTCAT GGCTTTGTGG GGGAATTTCC TGGAAGTTGT GTCACTAAAT 
GAGCGTGTTT TTGTGCTTTT GGCCCAGGAG CACCACACAC TACGGGTGCA AAGATTTGCT 
GAAGCTTTCT TTTGTCTTGA ACACCCACGA CAGAGTGCAC TGGCCTACCA GGAACTGCAT 
GCTCACAGTC ACCAACAAAT GACCACAGCT ATCAAAAGCT CTTCCTACTT CTTGTCCTTA 
CCACCTCTTC CTGTTGAATG TGCTGATCTT GACGGGGATG TGAACTCCCT ACCAATCATC 
TTTGAGGACA GATATTTGGA GTCTGTTACT GAAGATCGTG ATGGTCCATG GCTTGGACTC 
CAAAACAACA GGAGTGGCTC TGTGTCCGCC AAAGCAGATA GATCCAACTC AAAGGACTGC 
ATTGCCAGTG GAGCCCTCGG TCCGTATCCA GATAAGCAGC AAGCTTCCTT TGCCACTGCT 
TGGCCTGAAC AAAACGAGCC TTCCCTTAAA TCCAAAGCCA AGTCCATCAA GCTCAAGAAG 
AACACCAAGA CCGAAAACTC AAAGAAGCTG ATCAGACAGA GCTCGAAAGA TTCTGTGGTG 
CTCACAGGCT ACAAGAACTT GAAGGTTCCT CACAGTGAAA AGACCATTAA GTCCAAAGAC 
ATCGACCATC AATGCAATCA GAGAGATGAG CTAGAGGATG GAGGTAAATT CCTTCTTCAG 
AATGAAACCA CGACCACGCA TTGTGCTCAG AATGCTAAAG ACTGCACATC TGGCAACCTT 
CAAGGTAACG CCACAATCCT AAGTGACGGC TTTCCCAATC TTATGAGTTT GCAAATGCAG 
GCCAGGCCCC AGGCAGGTAC TGGAGCAGAG GCCGCCGCTG CCAGAGTAGA TCCCACACAT 
CCACCTGCTG GCCTCAGACA TATTGATGTC AAACCCAGTG ACAAGGACCC CTACCAGGGC 
GATAAGGTCA CCGTTCTCTT ACCGCACCAC CCAGAGCGCA CCACCAAGAG GGATGTTCCA 
GAAATCACAC AGACTGATTT CTCTGAGTTC AAGAGTCGAG TTGGAGATGG TGCTACTTTC 
GTCGGACCTT GTGGAGCGGC TTTGAGGAAC GATAATATTC CTCAAGCCTC CTCCAGACTC 
TCGGATTCCG GAATTGAAAG CGAACCCAGC TCTGCGACAC AGCTAGCCCC GGTTTCTCCA 
TCTCCTTTTG CTCCTATTGG GATCGAAGCA CCTACAGAGC TGATTCCTCA CAGACCACCT 
CCGCTCAGTC CTGCTTCCAG AGGAGCCACC GCATTGGAAG GCCTCAATCC TGAAAGCACC 
AGCGGCGTAA GCGGCGTCCA ATCTTCCCTC ACCTCCATCA ACTCACTCCC TTCGGATGAT 
GAGGTGGAGA CAGAGGCCAC CAGCAGGAAG TCCAGCATCC TGGTCCAGGA ACAGAGTCTC 
ATCTTCTCCG GAGAATCTCA AAGCCTCTTG CTGACAAACT CTAGAGTCTT GGCTCAACAA 
AAAGCCAGTC ATGGAGATGG GATGGACTCT GCTTTGCAGT CCCGCTGTGA AACTTCTGCT 
TCTGACAGAC TTGTCGGATC TAACGAGACA GATCTTCCAG GTGGTCCGGA AGCTTGTAGT 
GAACCACAGA CAGCAGCCCA GTCCAGTACA TCAGTCACCA GCAGCACAGA CATGGTGAAG 
AAAGGCTTTG TAGAAAACTA CTTTGGCTCT CGGTCCAGTA CTGATATATC TGAAATCAGC 
CCTTTAGAGA CGGCAGCCAT AACATTGGGT ATTCAGACAT GCCCCAATGC TAATGATGAA 
GAGGATGAGG AGGAAGAGCA GGAGCATGAG ATGATTGAGA ATGGTTATTA TGAGGAGACC 
GATGGTGTGG TTTATGTTAA TGGGCTCCCG GAGGATGAAG GGAGGTGTGA AGATGGAGGA 
GCGGAGGGAA GAACACTGTT TTATGATCAT CTTGGTTTAG AGCAGCTCCA GGAAACTTAC 
ATTCCCTCTG CCGGCATTCG GACACGGCCT ACTGGACCAG CACCAACCAG CAGCGACTTA 
TGCTGGTACA AGAGCGCCCC CACTGCTCAG ATGACGGCTT TTGTCCAAGC GAAAGAGGAG 
TTGAAACAAC TAAAGCTGCC AGGCTTCCTG TACAGTGAGG TGGCAGATCT GGCATCCACT 
GTGCCATATT TCAGCATGGA TGAGGATGAA AACTGTGATG ATGGCATACA CCTTATAGTG 
TGTGTACACG GCTTGGATGG TAACAGTGCA GATTTGCGCC TGGTGAAGAC TTATCTGGAG 
CTTGGGTTGC CTGGAGCTAG GATAGATTTT CTGATGTCTG AGCGAAATCA GAATGACACA 
TTTGCTGATT TTGAGTCCAT GACCGACAGA CTTTTGGATG AAATTGTGCA GTACATACAA 
ATCTACAACC TCACAGTTTC CAAGATCAGT TTTGTTGGTC ATTCTCTGGG AAATCTGATC 
GTGCGCTCTG TGCTGACGAG GCCCAGGTTT AAGTGTTATC TCAGCCGACT GCACACCTTT 
CTGTCTCTGT CTGGACCTCA CCTCGGCACA CTGTACAACA GCTCAGCACT CGTCAATACT 
GGACTGTGGT TCATGCAGAA GTGGAAGAAG TCTGGCTCTC TGCTGCAGCT GACCTGTCGA 
GACCATTCAG ACCCGCGTCA GACCTTTCTC TATAAGCTGA GCAAGAAAGC AGGCCTTCAG 
TTCTTCAAGA ATGTGGTGTT GGTGGGCTCT CTTCAGGACC GATACGTTCC TTATCACTCC 
GCTCGAATCG AGATGTGCAA AACAGCCTTA AAGGACAAAC AGACTGGTCC GGTGTATGCT 
GAGATGATCG AGAACCTTCT CCTTCCTGTG CTTCAGAACA AAGACTGTAA CCTGGTGCGT 
TACGACGTCA TCCACGCGCT GCCCAACACC GCCAACTCTC TGATCGGCCG CGCAGCTCAC 
ATCGCCGTCC TGGACTCTGA GATTTTCCTG GAAAAGTTCT TCCTGGTGGC AGGGCTCAAG 
TTTTTTCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214264.1
CDS	388..4419
Translation	MTEVQATVEFSVELHKFYNVDLFQRGFYQVRASMKVPPRIPQRVEASLLHPIGSDLAFPASVQDDVVCSKTFQILYKNEEIVVNDVFVFKVMMLLDAKKGVFLLQVEESLNEVDFQLCLDLFFTDGDYSPEEPSSLQSISSRTLRLHFSLHRGIHHHVNVMFDYFHLSVISATIHASLVALHQPLISFPRPVKSTWLNRNAPAQSKDSLIPPLETVVFGSTYVKQLSPDGKSFLVSDQCLQHAFSLHQNMCANLLRAYQSLFCYLTSLTKNLPASQRMELAKPSMQVLYERVLRRPYPPGQRHVYIEELDVEVRLHELCEQVKKAESPDELAELVNMNLAQLCSLLMALWGNFLEVVSLNERVFVLLAQEHHTLRVQRFAEAFFCLEHPRQSALAYQELHAHSHQQMTTAIKSSSYFLSLPPLPVECADLDGDVNSLPIIFEDRYLESVTEDRDGPWLGLQNNRSGSVSAKADRSNSKDCIASGALGPYPDKQQASFATAWPEQNEPSLKSKAKSIKLKKNTKTENSKKLIRQSSKDSVVLTGYKNLKVPHSEKTIKSKDIDHQCNQRDELEDGGKFLLQNETTTTHCAQNAKDCTSGNLQGNATILSDGFPNLMSLQMQARPQAGTGAEAAAARVDPTHPPAGLRHIDVKPSDKDPYQGDKVTVLLPHHPERTTKRDVPEITQTDFSEFKSRVGDGATFVGPCGAALRNDNIPQASSRLSDSGIESEPSSATQLAPVSPSPFAPIGIEAPTELIPHRPPPLSPASRGATALEGLNPESTSGVSGVQSSLTSINSLPSDDEVETEATSRKSSILVQEQSLIFSGESQSLLLTNSRVLAQQKASHGDGMDSALQSRCETSASDRLVGSNETDLPGGPEACSEPQTAAQSSTSVTSSTDMVKKGFVENYFGSRSSTDISEISPLETAAITLGIQTCPNANDEEDEEEEQEHEMIENGYYEETDGVVYVNGLPEDEGRCEDGGAEGRTLFYDHLGLEQLQETYIPSAGIRTRPTGPAPTSSDLCWYKSAPTAQMTAFVQAKEELKQLKLPGFLYSEVADLASTVPYFSMDEDENCDDGIHLIVCVHGLDGNSADLRLVKTYLELGLPGARIDFLMSERNQNDTFADFESMTDRLLDEIVQYIQIYNLTVSKISFVGHSLGNLIVRSVLTRPRFKCYLSRLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSKKAGLQFFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQTGPVYAEMIENLLLPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEIFLEKFFLVAGLKFFQ

Target ORF information:

RefSeq Version	XM_017358775.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio family with sequence similarity 135 member A (fam135a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358775.1

ORF Insert Sequence:

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGGT 
GTTTTCCTTC TGCAGGTAGA AGAGTCTTTA AATGAAGTTG ACTTTCAGCT GTGTTTGGAC 
CTGTTCTTCA CTGATGGAGA CTACTCGCCT GAGGAGCCCA GTTCTCTGCA GAGCATCAGC 
AGCCGTACGC TGCGTTTGCA CTTTAGCCTC CACAGAGGCA TCCACCACCA CGTCAACGTC 
ATGTTTGACT ACTTCCACCT GTCTGTCATC TCTGCCACAA TCCATGCTTC ACTGGTGGCT 
CTGCATCAGC CTCTAATAAG TTTCCCCCGG CCAGTAAAAA GCACCTGGTT GAATCGTAAT 
GCTCCGGCAC AGAGCAAAGA CTCCCTCATC CCACCGCTGG AGACTGTGGT GTTCGGCAGC 
ACCTACGTCA AGCAGCTGTC CCCTGATGGC AAAAGCTTCC TGGTATCGGA TCAGTGCCTC 
CAGCATGCCT TCAGCCTGCA CCAGAACATG TGTGCCAACC TGCTGCGGGC ATATCAGAGC 
CTGTTCTGTT ACTTAACCTC CCTGACCAAG AATCTGCCCG CATCCCAGCG CATGGAGCTT 
GCCAAGCCCA GCATGCAGGT CCTTTATGAA CGAGTTCTCA GAAGACCTTA CCCTCCAGGC 
CAAAGGCACG TCTACATAGA GGAACTTGAT GTGGAGGTCC GACTTCATGA GCTTTGTGAA 
CAAGTCAAGA AAGCAGAGAG CCCAGATGAG TTGGCTGAAT TGGTGAACAT GAATTTGGCG 
CAACTATGTT CCCTACTCAT GGCTTTGTGG GGGAATTTCC TGGAAGTTGT GTCACTAAAT 
GAGCGTGTTT TTGTGCTTTT GGCCCAGGAG CACCACACAC TACGGGTGCA AAGATTTGCT 
GAAGCTTTCT TTTGTCTTGA ACACCCACGA CAGAGTGCAC TGGCCTACCA GGAACTGCAT 
GCTCACAGTC ACCAACAAAT GACCACAGCT ATCAAAAGCT CTTCCTACTT CTTGTCCTTA 
CCACCTCTTC CTGTTGAATG TGCTGATCTT GACGGGGATG TGAACTCCCT ACCAATCATC 
TTTGAGGACA GATATTTGGA GTCTGTTACT GAAGATCGTG ATGGTCCATG GCTTGGACTC 
CAAAACAACA GGAGTGGCTC TGTGTCCGCC AAAGCAGATA GATCCAACTC AAAGGACTGC 
ATTGCCAGTG GAGCCCTCGG TCCGTATCCA GATAAGCAGC AAGCTTCCTT TGCCACTGCT 
TGGCCTGAAC AAAACGAGCC TTCCCTTAAA TCCAAAGCCA AGTCCATCAA GCTCAAGAAG 
AACACCAAGA CCGAAAACTC AAAGAAGCTG ATCAGACAGA GCTCGAAAGA TTCTGTGGTG 
CTCACAGGCT ACAAGAACTT GAAGGTTCCT CACAGTGAAA AGACCATTAA GTCCAAAGAC 
ATCGACCATC AATGCAATCA GAGAGATGAG CTAGAGGATG GAGGTAAATT CCTTCTTCAG 
AATGAAACCA CGACCACGCA TTGTGCTCAG AATGCTAAAG ACTGCACATC TGGCAACCTT 
CAAGGTAACG CCACAATCCT AAGTGACGGC TTTCCCAATC TTATGAGTTT GCAAATGCAG 
GCCAGGCCCC AGGCAGGTAC TGGAGCAGAG GCCGCCGCTG CCAGAGTAGA TCCCACACAT 
CCACCTGCTG GCCTCAGACA TATTGATGTC AAACCCAGTG ACAAGGACCC CTACCAGGGC 
GATAAGGTCA CCGTTCTCTT ACCGCACCAC CCAGAGCGCA CCACCAAGAG GGATGTTCCA 
GAAATCACAC AGACTGATTT CTCTGAGTTC AAGAGTCGAG TTGGAGATGG TGCTACTTTC 
GTCGGACCTT GTGGAGCGGC TTTGAGGAAC GATAATATTC CTCAAGCCTC CTCCAGACTC 
TCGGATTCCG GAATTGAAAG CGAACCCAGC TCTGCGACAC AGCTAGCCCC GGTTTCTCCA 
TCTCCTTTTG CTCCTATTGG GATCGAAGCA CCTACAGAGC TGATTCCTCA CAGACCACCT 
CCGCTCAGTC CTGCTTCCAG AGGAGCCACC GCATTGGAAG GCCTCAATCC TGAAAGCACC 
AGCGGCGTAA GCGGCGTCCA ATCTTCCCTC ACCTCCATCA ACTCACTCCC TTCGGATGAT 
GAGGTGGAGA CAGAGGCCAC CAGCAGGAAG TCCAGCATCC TGGTCCAGGA ACAGAGTCTC 
ATCTTCTCCG GAGAATCTCA AAGCCTCTTG CTGACAAACT CTAGAGTCTT GGCTCAACAA 
AAAGCCAGTC ATGGAGATGG GATGGACTCT GCTTTGCAGT CCCGCTGTGA AACTTCTGCT 
TCTGACAGAC TTGTCGGATC TAACGAGACA GATCTTCCAG GTGGTCCGGA AGCTTGTAGT 
GAACCACAGA CAGCAGCCCA GTCCAGTACA TCAGTCACCA GCAGCACAGA CATGGTGAAG 
AAAGGCTTTG TAGAAAACTA CTTTGGCTCT CGGTCCAGTA CTGATATATC TGAAATCAGC 
CCTTTAGAGA CGGCAGCCAT AACATTGGGT ATTCAGACAT GCCCCAATGC TAATGATGAA 
GAGGATGAGG AGGAAGAGCA GGAGCATGAG ATGATTGAGA ATGGTTATTA TGAGGAGACC 
GATGGTGTGG TTTATGTTAA TGGGCTCCCG GAGGATGAAG GGAGGTGTGA AGATGGAGGA 
GCGGAGGGAA GAACACTGTT TTATGATCAT CTTGGTTTAG AGCAGCTCCA GGAAACTTAC 
ATTCCCTCTG CCGGCATTCG GACACGGCCT ACTGGACCAG CACCAACCAG CAGCGACTTA 
TGCTGGTACA AGAGCGCCCC CACTGCTCAG ATGACGGCTT TTGTCCAAGC GAAAGAGGAG 
TTGAAACAAC TAAAGCTGCC AGGCTTCCTG TACAGTGAGG TGGCAGATCT GGCATCCACT 
GTGCCATATT TCAGCATGGA TGAGGATGAA AACTGTGATG ATGGCATACA CCTTATAGTG 
TGTGTACACG GCTTGGATGG TAACAGTGCA GATTTGCGCC TGGTGAAGAC TTATCTGGAG 
CTTGGGTTGC CTGGAGCTAG GATAGATTTT CTGATGTCTG AGCGAAATCA GAATGACACA 
TTTGCTGATT TTGAGTCCAT GACCGACAGA CTTTTGGATG AAATTGTGCA GTACATACAA 
ATCTACAACC TCACAGTTTC CAAGATCAGT TTTGTTGGTC ATTCTCTGGG AAATCTGATC 
GTGCGCTCTG TGCTGACGAG GCCCAGGTTT AAGTGTTATC TCAGCCGACT GCACACCTTT 
CTGTCTCTGT CTGGACCTCA CCTCGGCACA CTGTACAACA GCTCAGCACT CGTCAATACT 
GGACTGTGGT TCATGCAGAA GTGGAAGAAG TCTGGCTCTC TGCTGCAGCT GACCTGTCGA 
GACCATTCAG ACCCGCGTCA GACCTTTCTC TATAAGCTGA GCAAGAAAGC AGGCCTTCAG 
TTCTTCAAGA ATGTGGTGTT GGTGGGCTCT CTTCAGGACC GATACGTTCC TTATCACTCC 
GCTCGAATCG AGATGTGCAA AACAGCCTTA AAGGACAAAC AGACTGGTCC GGTGTATGCT 
GAGATGATCG AGAACCTTCT CCTTCCTGTG CTTCAGAACA AAGACTGTAA CCTGGTGCGT 
TACGACGTCA TCCACGCGCT GCCCAACACC GCCAACTCTC TGATCGGCCG CGCAGCTCAC 
ATCGCCGTCC TGGACTCTGA GATTTTCCTG GAAAAGTTCT TCCTGGTGGC AGGGCTCAAG 
TTTTTTCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam135a ( XM_017358776.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214265.1 Danio rerio family with sequence similarity 135 member A (fam135a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa54722
Clone ID Related Accession (Same CDS sequence)	XM_017358776.1 , XM_017358776.2
Accession Version	XM_017358776.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein FAM135A isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGGT 
GTTTTCCTTC TGCAGGTAGA AGAGTCTTTA AATGAAGTTG ACTTTCAGCT GTGTTTGGAC 
CTGTTCTTCA CTGATGGAGA CTACTCGCCT GAGGAGCCCA GTTCTCTGCA GAGCATCAGC 
AGCCGTACGC TGCGTTTGCA CTTTAGCCTC CACAGAGGCA TCCACCACCA CGTCAACGTC 
ATGTTTGACT ACTTCCACCT GTCTGTCATC TCTGCCACAA TCCATGCTTC ACTGGTGGCT 
CTGCATCAGC CTCTAATAAG TTTCCCCCGG CCAGTAAAAA GCACCTGGTT GAATCGTAAT 
GCTCCGGCAC AGAGCAAAGA CTCCCTCATC CCACCGCTGG AGACTGTGGT GTTCGGCAGC 
ACCTACGTCA AGCAGCTGTC CCCTGATGGC AAAAGCTTCC TGGTATCGGA TCAGTGCCTC 
CAGCATGCCT TCAGCCTGCA CCAGAACATG TGTGCCAACC TGCTGCGGGC ATATCAGAGC 
CTGTTCTGTT ACTTAACCTC CCTGACCAAG AATCTGCCCG CATCCCAGCG CATGGAGCTT 
GAGGAACTTG ATGTGGAGGT CCGACTTCAT GAGCTTTGTG AACAAGTCAA GCAGAAAGCA 
GAGAGCCCAG ATGAGTTGGC TGAATTGGTG AACATGAATT TGGCGCAACT ATGTTCCCTA 
CTCATGGCTT TGTGGGGGAA TTTCCTGGAA GTTGTGTCAC TAAATGAGCG TGTTTTTGTG 
CTTTTGGCCC AGGAGCACCA CACACTACGG GTGCAAAGAT TTGCTGAAGC TTTCTTTTGT 
CTTGAACACC CACGACAGAG TGCACTGGCC TACCAGGAAC TGCATGCTCA CAGTCACCAA 
CAAATGACCA CAGCTATCAA AAGCTCTTCC TACTTCTTGT CCTTACCACC TCTTCCTGTT 
GAATGTGCTG ATCTTGACGG GGATGTGAAC TCCCTACCAA TCATCTTTGA GGACAGATAT 
TTGGAGTCTG TTACTGAAGA TCGTGATGGT CCATGGCTTG GACTCCAAAA CAACAGGAGT 
GGCTCTGTGT CCGCCAAAGC AGATAGATCC AACTCAAAGG ACTGCATTGC CAGTGGAGCC 
CTCGGTCCGT ATCCAGATAA GCAGCAAGCT TCCTTTGCCA CTGCTTGGCC TGAACAAAAC 
GAGCCTTCCC TTAAATCCAA AGCCAAGTCC ATCAAGCTCA AGAAGAACAC CAAGACCGAA 
AACTCAAAGA AGCTGATCAG ACAGAGCTCG AAAGATTCTG TGGTGCTCAC AGGCTACAAG 
AACTTGAAGG TTCCTCACAG TGAAAAGACC ATTAAGTCCA AAGACATCGA CCATCAATGC 
AATCAGAGAG ATGAGCTAGA GGATGGAGGT AAATTCCTTC TTCAGAATGA AACCACGACC 
ACGCATTGTG CTCAGAATGC TAAAGACTGC ACATCTGGCA ACCTTCAAGG TAACGCCACA 
ATCCTAAGTG ACGGCTTTCC CAATCTTATG AGTTTGCAAA TGCAGGCCAG GCCCCAGGCA 
GGTACTGGAG CAGAGGCCGC CGCTGCCAGA GTAGATCCCA CACATCCACC TGCTGGCCTC 
AGACATATTG ATGTCAAACC CAGTGACAAG GACCCCTACC AGGGCGATAA GGTCACCGTT 
CTCTTACCGC ACCACCCAGA GCGCACCACC AAGAGGGATG TTCCAGAAAT CACACAGACT 
GATTTCTCTG AGTTCAAGAG TCGAGTTGGA GATGGTGCTA CTTTCGTCGG ACCTTGTGGA 
GCGGCTTTGA GGAACGATAA TATTCCTCAA GCCTCCTCCA GACTCTCGGA TTCCGGAATT 
GAAAGCGAAC CCAGCTCTGC GACACAGCTA GCCCCGGTTT CTCCATCTCC TTTTGCTCCT 
ATTGGGATCG AAGCACCTAC AGAGCTGATT CCTCACAGAC CACCTCCGCT CAGTCCTGCT 
TCCAGAGGAG CCACCGCATT GGAAGGCCTC AATCCTGAAA GCACCAGCGG CGTAAGCGGC 
GTCCAATCTT CCCTCACCTC CATCAACTCA CTCCCTTCGG ATGATGAGGT GGAGACAGAG 
GCCACCAGCA GGAAGTCCAG CATCCTGGTC CAGGAACAGA GTCTCATCTT CTCCGGAGAA 
TCTCAAAGCC TCTTGCTGAC AAACTCTAGA GTCTTGGCTC AACAAAAAGC CAGTCATGGA 
GATGGGATGG ACTCTGCTTT GCAGTCCCGC TGTGAAACTT CTGCTTCTGA CAGACTTGTC 
GGATCTAACG AGACAGATCT TCCAGGTGGT CCGGAAGCTT GTAGTGAACC ACAGACAGCA 
GCCCAGTCCA GTACATCAGT CACCAGCAGC ACAGACATGG TGAAGAAAGG CTTTGTAGAA 
AACTACTTTG GCTCTCGGTC CAGTACTGAT ATATCTGAAA TCAGCCCTTT AGAGACGGCA 
GCCATAACAT TGGGTATTCA GACATGCCCC AATGCTAATG ATGAAGAGGA TGAGGAGGAA 
GAGCAGGAGC ATGAGATGAT TGAGAATGGT TATTATGAGG AGACCGATGG TGTGGTTTAT 
GTTAATGGGC TCCCGGAGGA TGAAGGGAGG TGTGAAGATG GAGGAGCGGA GGGAAGAACA 
CTGTTTTATG ATCATCTTGG TTTAGAGCAG CTCCAGGAAA CTTACATTCC CTCTGCCGGC 
ATTCGGACAC GGCCTACTGG ACCAGCACCA ACCAGCAGCG ACTTATGCTG GTACAAGAGC 
GCCCCCACTG CTCAGATGAC GGCTTTTGTC CAAGCGAAAG AGGAGTTGAA ACAACTAAAG 
CTGCCAGGCT TCCTGTACAG TGAGGTGGCA GATCTGGCAT CCACTGTGCC ATATTTCAGC 
ATGGATGAGG ATGAAAACTG TGATGATGGC ATACACCTTA TAGTGTGTGT ACACGGCTTG 
GATGGTAACA GTGCAGATTT GCGCCTGGTG AAGACTTATC TGGAGCTTGG GTTGCCTGGA 
GCTAGGATAG ATTTTCTGAT GTCTGAGCGA AATCAGAATG ACACATTTGC TGATTTTGAG 
TCCATGACCG ACAGACTTTT GGATGAAATT GTGCAGTACA TACAAATCTA CAACCTCACA 
GTTTCCAAGA TCAGTTTTGT TGGTCATTCT CTGGGAAATC TGATCGTGCG CTCTGTGCTG 
ACGAGGCCCA GGTTTAAGTG TTATCTCAGC CGACTGCACA CCTTTCTGTC TCTGTCTGGA 
CCTCACCTCG GCACACTGTA CAACAGCTCA GCACTCGTCA ATACTGGACT GTGGTTCATG 
CAGAAGTGGA AGAAGTCTGG CTCTCTGCTG CAGCTGACCT GTCGAGACCA TTCAGACCCG 
CGTCAGACCT TTCTCTATAA GCTGAGCAAG AAAGCAGGCC TTCAGTTCTT CAAGAATGTG 
GTGTTGGTGG GCTCTCTTCA GGACCGATAC GTTCCTTATC ACTCCGCTCG AATCGAGATG 
TGCAAAACAG CCTTAAAGGA CAAACAGACT GGTCCGGTGT ATGCTGAGAT GATCGAGAAC 
CTTCTCCTTC CTGTGCTTCA GAACAAAGAC TGTAACCTGG TGCGTTACGA CGTCATCCAC 
GCGCTGCCCA ACACCGCCAA CTCTCTGATC GGCCGCGCAG CTCACATCGC CGTCCTGGAC 
TCTGAGATTT TCCTGGAAAA GTTCTTCCTG GTGGCAGGGC TCAAGTTTTT TCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214265.1
CDS	388..4344
Translation	MTEVQATVEFSVELHKFYNVDLFQRGFYQVRASMKVPPRIPQRVEASLLHPIGSDLAFPASVQDDVVCSKTFQILYKNEEIVVNDVFVFKVMMLLDAKKGVFLLQVEESLNEVDFQLCLDLFFTDGDYSPEEPSSLQSISSRTLRLHFSLHRGIHHHVNVMFDYFHLSVISATIHASLVALHQPLISFPRPVKSTWLNRNAPAQSKDSLIPPLETVVFGSTYVKQLSPDGKSFLVSDQCLQHAFSLHQNMCANLLRAYQSLFCYLTSLTKNLPASQRMELEELDVEVRLHELCEQVKQKAESPDELAELVNMNLAQLCSLLMALWGNFLEVVSLNERVFVLLAQEHHTLRVQRFAEAFFCLEHPRQSALAYQELHAHSHQQMTTAIKSSSYFLSLPPLPVECADLDGDVNSLPIIFEDRYLESVTEDRDGPWLGLQNNRSGSVSAKADRSNSKDCIASGALGPYPDKQQASFATAWPEQNEPSLKSKAKSIKLKKNTKTENSKKLIRQSSKDSVVLTGYKNLKVPHSEKTIKSKDIDHQCNQRDELEDGGKFLLQNETTTTHCAQNAKDCTSGNLQGNATILSDGFPNLMSLQMQARPQAGTGAEAAAARVDPTHPPAGLRHIDVKPSDKDPYQGDKVTVLLPHHPERTTKRDVPEITQTDFSEFKSRVGDGATFVGPCGAALRNDNIPQASSRLSDSGIESEPSSATQLAPVSPSPFAPIGIEAPTELIPHRPPPLSPASRGATALEGLNPESTSGVSGVQSSLTSINSLPSDDEVETEATSRKSSILVQEQSLIFSGESQSLLLTNSRVLAQQKASHGDGMDSALQSRCETSASDRLVGSNETDLPGGPEACSEPQTAAQSSTSVTSSTDMVKKGFVENYFGSRSSTDISEISPLETAAITLGIQTCPNANDEEDEEEEQEHEMIENGYYEETDGVVYVNGLPEDEGRCEDGGAEGRTLFYDHLGLEQLQETYIPSAGIRTRPTGPAPTSSDLCWYKSAPTAQMTAFVQAKEELKQLKLPGFLYSEVADLASTVPYFSMDEDENCDDGIHLIVCVHGLDGNSADLRLVKTYLELGLPGARIDFLMSERNQNDTFADFESMTDRLLDEIVQYIQIYNLTVSKISFVGHSLGNLIVRSVLTRPRFKCYLSRLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSKKAGLQFFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQTGPVYAEMIENLLLPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEIFLEKFFLVAGLKFFQ

Target ORF information:

RefSeq Version	XM_017358776.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio family with sequence similarity 135 member A (fam135a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358776.1

ORF Insert Sequence:

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGGT 
GTTTTCCTTC TGCAGGTAGA AGAGTCTTTA AATGAAGTTG ACTTTCAGCT GTGTTTGGAC 
CTGTTCTTCA CTGATGGAGA CTACTCGCCT GAGGAGCCCA GTTCTCTGCA GAGCATCAGC 
AGCCGTACGC TGCGTTTGCA CTTTAGCCTC CACAGAGGCA TCCACCACCA CGTCAACGTC 
ATGTTTGACT ACTTCCACCT GTCTGTCATC TCTGCCACAA TCCATGCTTC ACTGGTGGCT 
CTGCATCAGC CTCTAATAAG TTTCCCCCGG CCAGTAAAAA GCACCTGGTT GAATCGTAAT 
GCTCCGGCAC AGAGCAAAGA CTCCCTCATC CCACCGCTGG AGACTGTGGT GTTCGGCAGC 
ACCTACGTCA AGCAGCTGTC CCCTGATGGC AAAAGCTTCC TGGTATCGGA TCAGTGCCTC 
CAGCATGCCT TCAGCCTGCA CCAGAACATG TGTGCCAACC TGCTGCGGGC ATATCAGAGC 
CTGTTCTGTT ACTTAACCTC CCTGACCAAG AATCTGCCCG CATCCCAGCG CATGGAGCTT 
GAGGAACTTG ATGTGGAGGT CCGACTTCAT GAGCTTTGTG AACAAGTCAA GCAGAAAGCA 
GAGAGCCCAG ATGAGTTGGC TGAATTGGTG AACATGAATT TGGCGCAACT ATGTTCCCTA 
CTCATGGCTT TGTGGGGGAA TTTCCTGGAA GTTGTGTCAC TAAATGAGCG TGTTTTTGTG 
CTTTTGGCCC AGGAGCACCA CACACTACGG GTGCAAAGAT TTGCTGAAGC TTTCTTTTGT 
CTTGAACACC CACGACAGAG TGCACTGGCC TACCAGGAAC TGCATGCTCA CAGTCACCAA 
CAAATGACCA CAGCTATCAA AAGCTCTTCC TACTTCTTGT CCTTACCACC TCTTCCTGTT 
GAATGTGCTG ATCTTGACGG GGATGTGAAC TCCCTACCAA TCATCTTTGA GGACAGATAT 
TTGGAGTCTG TTACTGAAGA TCGTGATGGT CCATGGCTTG GACTCCAAAA CAACAGGAGT 
GGCTCTGTGT CCGCCAAAGC AGATAGATCC AACTCAAAGG ACTGCATTGC CAGTGGAGCC 
CTCGGTCCGT ATCCAGATAA GCAGCAAGCT TCCTTTGCCA CTGCTTGGCC TGAACAAAAC 
GAGCCTTCCC TTAAATCCAA AGCCAAGTCC ATCAAGCTCA AGAAGAACAC CAAGACCGAA 
AACTCAAAGA AGCTGATCAG ACAGAGCTCG AAAGATTCTG TGGTGCTCAC AGGCTACAAG 
AACTTGAAGG TTCCTCACAG TGAAAAGACC ATTAAGTCCA AAGACATCGA CCATCAATGC 
AATCAGAGAG ATGAGCTAGA GGATGGAGGT AAATTCCTTC TTCAGAATGA AACCACGACC 
ACGCATTGTG CTCAGAATGC TAAAGACTGC ACATCTGGCA ACCTTCAAGG TAACGCCACA 
ATCCTAAGTG ACGGCTTTCC CAATCTTATG AGTTTGCAAA TGCAGGCCAG GCCCCAGGCA 
GGTACTGGAG CAGAGGCCGC CGCTGCCAGA GTAGATCCCA CACATCCACC TGCTGGCCTC 
AGACATATTG ATGTCAAACC CAGTGACAAG GACCCCTACC AGGGCGATAA GGTCACCGTT 
CTCTTACCGC ACCACCCAGA GCGCACCACC AAGAGGGATG TTCCAGAAAT CACACAGACT 
GATTTCTCTG AGTTCAAGAG TCGAGTTGGA GATGGTGCTA CTTTCGTCGG ACCTTGTGGA 
GCGGCTTTGA GGAACGATAA TATTCCTCAA GCCTCCTCCA GACTCTCGGA TTCCGGAATT 
GAAAGCGAAC CCAGCTCTGC GACACAGCTA GCCCCGGTTT CTCCATCTCC TTTTGCTCCT 
ATTGGGATCG AAGCACCTAC AGAGCTGATT CCTCACAGAC CACCTCCGCT CAGTCCTGCT 
TCCAGAGGAG CCACCGCATT GGAAGGCCTC AATCCTGAAA GCACCAGCGG CGTAAGCGGC 
GTCCAATCTT CCCTCACCTC CATCAACTCA CTCCCTTCGG ATGATGAGGT GGAGACAGAG 
GCCACCAGCA GGAAGTCCAG CATCCTGGTC CAGGAACAGA GTCTCATCTT CTCCGGAGAA 
TCTCAAAGCC TCTTGCTGAC AAACTCTAGA GTCTTGGCTC AACAAAAAGC CAGTCATGGA 
GATGGGATGG ACTCTGCTTT GCAGTCCCGC TGTGAAACTT CTGCTTCTGA CAGACTTGTC 
GGATCTAACG AGACAGATCT TCCAGGTGGT CCGGAAGCTT GTAGTGAACC ACAGACAGCA 
GCCCAGTCCA GTACATCAGT CACCAGCAGC ACAGACATGG TGAAGAAAGG CTTTGTAGAA 
AACTACTTTG GCTCTCGGTC CAGTACTGAT ATATCTGAAA TCAGCCCTTT AGAGACGGCA 
GCCATAACAT TGGGTATTCA GACATGCCCC AATGCTAATG ATGAAGAGGA TGAGGAGGAA 
GAGCAGGAGC ATGAGATGAT TGAGAATGGT TATTATGAGG AGACCGATGG TGTGGTTTAT 
GTTAATGGGC TCCCGGAGGA TGAAGGGAGG TGTGAAGATG GAGGAGCGGA GGGAAGAACA 
CTGTTTTATG ATCATCTTGG TTTAGAGCAG CTCCAGGAAA CTTACATTCC CTCTGCCGGC 
ATTCGGACAC GGCCTACTGG ACCAGCACCA ACCAGCAGCG ACTTATGCTG GTACAAGAGC 
GCCCCCACTG CTCAGATGAC GGCTTTTGTC CAAGCGAAAG AGGAGTTGAA ACAACTAAAG 
CTGCCAGGCT TCCTGTACAG TGAGGTGGCA GATCTGGCAT CCACTGTGCC ATATTTCAGC 
ATGGATGAGG ATGAAAACTG TGATGATGGC ATACACCTTA TAGTGTGTGT ACACGGCTTG 
GATGGTAACA GTGCAGATTT GCGCCTGGTG AAGACTTATC TGGAGCTTGG GTTGCCTGGA 
GCTAGGATAG ATTTTCTGAT GTCTGAGCGA AATCAGAATG ACACATTTGC TGATTTTGAG 
TCCATGACCG ACAGACTTTT GGATGAAATT GTGCAGTACA TACAAATCTA CAACCTCACA 
GTTTCCAAGA TCAGTTTTGT TGGTCATTCT CTGGGAAATC TGATCGTGCG CTCTGTGCTG 
ACGAGGCCCA GGTTTAAGTG TTATCTCAGC CGACTGCACA CCTTTCTGTC TCTGTCTGGA 
CCTCACCTCG GCACACTGTA CAACAGCTCA GCACTCGTCA ATACTGGACT GTGGTTCATG 
CAGAAGTGGA AGAAGTCTGG CTCTCTGCTG CAGCTGACCT GTCGAGACCA TTCAGACCCG 
CGTCAGACCT TTCTCTATAA GCTGAGCAAG AAAGCAGGCC TTCAGTTCTT CAAGAATGTG 
GTGTTGGTGG GCTCTCTTCA GGACCGATAC GTTCCTTATC ACTCCGCTCG AATCGAGATG 
TGCAAAACAG CCTTAAAGGA CAAACAGACT GGTCCGGTGT ATGCTGAGAT GATCGAGAAC 
CTTCTCCTTC CTGTGCTTCA GAACAAAGAC TGTAACCTGG TGCGTTACGA CGTCATCCAC 
GCGCTGCCCA ACACCGCCAA CTCTCTGATC GGCCGCGCAG CTCACATCGC CGTCCTGGAC 
TCTGAGATTT TCCTGGAAAA GTTCTTCCTG GTGGCAGGGC TCAAGTTTTT TCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam135a ( XM_017358776.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214265.1 Danio rerio family with sequence similarity 135, member A (fam135a), transcript variant X5, mRNA.

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CloneID	ODa54722
Clone ID Related Accession (Same CDS sequence)	XM_017358776.1 , XM_017358776.2
Accession Version	XM_017358776.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3957bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein FAM135A isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358776.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGGT 
GTTTTCCTTC TGCAGGTAGA AGAGTCTTTA AATGAAGTTG ACTTTCAGCT GTGTTTGGAC 
CTGTTCTTCA CTGATGGAGA CTACTCGCCT GAGGAGCCCA GTTCTCTGCA GAGCATCAGC 
AGCCGTACGC TGCGTTTGCA CTTTAGCCTC CACAGAGGCA TCCACCACCA CGTCAACGTC 
ATGTTTGACT ACTTCCACCT GTCTGTCATC TCTGCCACAA TCCATGCTTC ACTGGTGGCT 
CTGCATCAGC CTCTAATAAG TTTCCCCCGG CCAGTAAAAA GCACCTGGTT GAATCGTAAT 
GCTCCGGCAC AGAGCAAAGA CTCCCTCATC CCACCGCTGG AGACTGTGGT GTTCGGCAGC 
ACCTACGTCA AGCAGCTGTC CCCTGATGGC AAAAGCTTCC TGGTATCGGA TCAGTGCCTC 
CAGCATGCCT TCAGCCTGCA CCAGAACATG TGTGCCAACC TGCTGCGGGC ATATCAGAGC 
CTGTTCTGTT ACTTAACCTC CCTGACCAAG AATCTGCCCG CATCCCAGCG CATGGAGCTT 
GAGGAACTTG ATGTGGAGGT CCGACTTCAT GAGCTTTGTG AACAAGTCAA GCAGAAAGCA 
GAGAGCCCAG ATGAGTTGGC TGAATTGGTG AACATGAATT TGGCGCAACT ATGTTCCCTA 
CTCATGGCTT TGTGGGGGAA TTTCCTGGAA GTTGTGTCAC TAAATGAGCG TGTTTTTGTG 
CTTTTGGCCC AGGAGCACCA CACACTACGG GTGCAAAGAT TTGCTGAAGC TTTCTTTTGT 
CTTGAACACC CACGACAGAG TGCACTGGCC TACCAGGAAC TGCATGCTCA CAGTCACCAA 
CAAATGACCA CAGCTATCAA AAGCTCTTCC TACTTCTTGT CCTTACCACC TCTTCCTGTT 
GAATGTGCTG ATCTTGACGG GGATGTGAAC TCCCTACCAA TCATCTTTGA GGACAGATAT 
TTGGAGTCTG TTACTGAAGA TCGTGATGGT CCATGGCTTG GACTCCAAAA CAACAGGAGT 
GGCTCTGTGT CCGCCAAAGC AGATAGATCC AACTCAAAGG ACTGCATTGC CAGTGGAGCC 
CTCGGTCCGT ATCCAGATAA GCAGCAAGCT TCCTTTGCCA CTGCTTGGCC TGAACAAAAC 
GAGCCTTCCC TTAAATCCAA AGCCAAGTCC ATCAAGCTCA AGAAGAACAC CAAGACCGAA 
AACTCAAAGA AGCTGATCAG ACAGAGCTCG AAAGATTCTG TGGTGCTCAC AGGCTACAAG 
AACTTGAAGG TTCCTCACAG TGAAAAGACC ATTAAGTCCA AAGACATCGA CCATCAATGC 
AATCAGAGAG ATGAGCTAGA GGATGGAGGT AAATTCCTTC TTCAGAATGA AACCACGACC 
ACGCATTGTG CTCAGAATGC TAAAGACTGC ACATCTGGCA ACCTTCAAGG TAACGCCACA 
ATCCTAAGTG ACGGCTTTCC CAATCTTATG AGTTTGCAAA TGCAGGCCAG GCCCCAGGCA 
GGTACTGGAG CAGAGGCCGC CGCTGCCAGA GTAGATCCCA CACATCCACC TGCTGGCCTC 
AGACATATTG ATGTCAAACC CAGTGACAAG GACCCCTACC AGGGCGATAA GGTCACCGTT 
CTCTTACCGC ACCACCCAGA GCGCACCACC AAGAGGGATG TTCCAGAAAT CACACAGACT 
GATTTCTCTG AGTTCAAGAG TCGAGTTGGA GATGGTGCTA CTTTCGTCGG ACCTTGTGGA 
GCGGCTTTGA GGAACGATAA TATTCCTCAA GCCTCCTCCA GACTCTCGGA TTCCGGAATT 
GAAAGCGAAC CCAGCTCTGC GACACAGCTA GCCCCGGTTT CTCCATCTCC TTTTGCTCCT 
ATTGGGATCG AAGCACCTAC AGAGCTGATT CCTCACAGAC CACCTCCGCT CAGTCCTGCT 
TCCAGAGGAG CCACCGCATT GGAAGGCCTC AATCCTGAAA GCACCAGCGG CGTAAGCGGC 
GTCCAATCTT CCCTCACCTC CATCAACTCA CTCCCTTCGG ATGATGAGGT GGAGACAGAG 
GCCACCAGCA GGAAGTCCAG CATCCTGGTC CAGGAACAGA GTCTCATCTT CTCCGGAGAA 
TCTCAAAGCC TCTTGCTGAC AAACTCTAGA GTCTTGGCTC AACAAAAAGC CAGTCATGGA 
GATGGGATGG ACTCTGCTTT GCAGTCCCGC TGTGAAACTT CTGCTTCTGA CAGACTTGTC 
GGATCTAACG AGACAGATCT TCCAGGTGGT CCGGAAGCTT GTAGTGAACC ACAGACAGCA 
GCCCAGTCCA GTACATCAGT CACCAGCAGC ACAGACATGG TGAAGAAAGG CTTTGTAGAA 
AACTACTTTG GCTCTCGGTC CAGTACTGAT ATATCTGAAA TCAGCCCTTT AGAGACGGCA 
GCCATAACAT TGGGTATTCA GACATGCCCC AATGCTAATG ATGAAGAGGA TGAGGAGGAA 
GAGCAGGAGC ATGAGATGAT TGAGAATGGT TATTATGAGG AGACCGATGG TGTGGTTTAT 
GTTAATGGGC TCCCGGAGGA TGAAGGGAGG TGTGAAGATG GAGGAGCGGA GGGAAGAACA 
CTGTTTTATG ATCATCTTGG TTTAGAGCAG CTCCAGGAAA CTTACATTCC CTCTGCCGGC 
ATTCGGACAC GGCCTACTGG ACCAGCACCA ACCAGCAGCG ACTTATGCTG GTACAAGAGC 
GCCCCCACTG CTCAGATGAC GGCTTTTGTC CAAGCGAAAG AGGAGTTGAA ACAACTAAAG 
CTGCCAGGCT TCCTGTACAG TGAGGTGGCA GATCTGGCAT CCACTGTGCC ATATTTCAGC 
ATGGATGAGG ATGAAAACTG TGATGATGGC ATACACCTTA TAGTGTGTGT ACACGGCTTG 
GATGGTAACA GTGCAGATTT GCGCCTGGTG AAGACTTATC TGGAGCTTGG GTTGCCTGGA 
GCTAGGATAG ATTTTCTGAT GTCTGAGCGA AATCAGAATG ACACATTTGC TGATTTTGAG 
TCCATGACCG ACAGACTTTT GGATGAAATT GTGCAGTACA TACAAATCTA CAACCTCACA 
GTTTCCAAGA TCAGTTTTGT TGGTCATTCT CTGGGAAATC TGATCGTGCG CTCTGTGCTG 
ACGAGGCCCA GGTTTAAGTG TTATCTCAGC CGACTGCACA CCTTTCTGTC TCTGTCTGGA 
CCTCACCTCG GCACACTGTA CAACAGCTCA GCACTCGTCA ATACTGGACT GTGGTTCATG 
CAGAAGTGGA AGAAGTCTGG CTCTCTGCTG CAGCTGACCT GTCGAGACCA TTCAGACCCG 
CGTCAGACCT TTCTCTATAA GCTGAGCAAG AAAGCAGGCC TTCAGTTCTT CAAGAATGTG 
GTGTTGGTGG GCTCTCTTCA GGACCGATAC GTTCCTTATC ACTCCGCTCG AATCGAGATG 
TGCAAAACAG CCTTAAAGGA CAAACAGACT GGTCCGGTGT ATGCTGAGAT GATCGAGAAC 
CTTCTCCTTC CTGTGCTTCA GAACAAAGAC TGTAACCTGG TGCGTTACGA CGTCATCCAC 
GCGCTGCCCA ACACCGCCAA CTCTCTGATC GGCCGCGCAG CTCACATCGC CGTCCTGGAC 
TCTGAGATTT TCCTGGAAAA GTTCTTCCTG GTGGCAGGGC TCAAGTTTTT TCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214265.1
CDS	405..4361
Translation	MTEVQATVEFSVELHKFYNVDLFQRGFYQVRASMKVPPRIPQRVEASLLHPIGSDLAFPASVQDDVVCSKTFQILYKNEEIVVNDVFVFKVMMLLDAKKGVFLLQVEESLNEVDFQLCLDLFFTDGDYSPEEPSSLQSISSRTLRLHFSLHRGIHHHVNVMFDYFHLSVISATIHASLVALHQPLISFPRPVKSTWLNRNAPAQSKDSLIPPLETVVFGSTYVKQLSPDGKSFLVSDQCLQHAFSLHQNMCANLLRAYQSLFCYLTSLTKNLPASQRMELEELDVEVRLHELCEQVKQKAESPDELAELVNMNLAQLCSLLMALWGNFLEVVSLNERVFVLLAQEHHTLRVQRFAEAFFCLEHPRQSALAYQELHAHSHQQMTTAIKSSSYFLSLPPLPVECADLDGDVNSLPIIFEDRYLESVTEDRDGPWLGLQNNRSGSVSAKADRSNSKDCIASGALGPYPDKQQASFATAWPEQNEPSLKSKAKSIKLKKNTKTENSKKLIRQSSKDSVVLTGYKNLKVPHSEKTIKSKDIDHQCNQRDELEDGGKFLLQNETTTTHCAQNAKDCTSGNLQGNATILSDGFPNLMSLQMQARPQAGTGAEAAAARVDPTHPPAGLRHIDVKPSDKDPYQGDKVTVLLPHHPERTTKRDVPEITQTDFSEFKSRVGDGATFVGPCGAALRNDNIPQASSRLSDSGIESEPSSATQLAPVSPSPFAPIGIEAPTELIPHRPPPLSPASRGATALEGLNPESTSGVSGVQSSLTSINSLPSDDEVETEATSRKSSILVQEQSLIFSGESQSLLLTNSRVLAQQKASHGDGMDSALQSRCETSASDRLVGSNETDLPGGPEACSEPQTAAQSSTSVTSSTDMVKKGFVENYFGSRSSTDISEISPLETAAITLGIQTCPNANDEEDEEEEQEHEMIENGYYEETDGVVYVNGLPEDEGRCEDGGAEGRTLFYDHLGLEQLQETYIPSAGIRTRPTGPAPTSSDLCWYKSAPTAQMTAFVQAKEELKQLKLPGFLYSEVADLASTVPYFSMDEDENCDDGIHLIVCVHGLDGNSADLRLVKTYLELGLPGARIDFLMSERNQNDTFADFESMTDRLLDEIVQYIQIYNLTVSKISFVGHSLGNLIVRSVLTRPRFKCYLSRLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSKKAGLQFFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQTGPVYAEMIENLLLPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEIFLEKFFLVAGLKFFQ

Target ORF information:

RefSeq Version	XM_017358776.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio family with sequence similarity 135, member A (fam135a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358776.2

ORF Insert Sequence:

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGGT 
GTTTTCCTTC TGCAGGTAGA AGAGTCTTTA AATGAAGTTG ACTTTCAGCT GTGTTTGGAC 
CTGTTCTTCA CTGATGGAGA CTACTCGCCT GAGGAGCCCA GTTCTCTGCA GAGCATCAGC 
AGCCGTACGC TGCGTTTGCA CTTTAGCCTC CACAGAGGCA TCCACCACCA CGTCAACGTC 
ATGTTTGACT ACTTCCACCT GTCTGTCATC TCTGCCACAA TCCATGCTTC ACTGGTGGCT 
CTGCATCAGC CTCTAATAAG TTTCCCCCGG CCAGTAAAAA GCACCTGGTT GAATCGTAAT 
GCTCCGGCAC AGAGCAAAGA CTCCCTCATC CCACCGCTGG AGACTGTGGT GTTCGGCAGC 
ACCTACGTCA AGCAGCTGTC CCCTGATGGC AAAAGCTTCC TGGTATCGGA TCAGTGCCTC 
CAGCATGCCT TCAGCCTGCA CCAGAACATG TGTGCCAACC TGCTGCGGGC ATATCAGAGC 
CTGTTCTGTT ACTTAACCTC CCTGACCAAG AATCTGCCCG CATCCCAGCG CATGGAGCTT 
GAGGAACTTG ATGTGGAGGT CCGACTTCAT GAGCTTTGTG AACAAGTCAA GCAGAAAGCA 
GAGAGCCCAG ATGAGTTGGC TGAATTGGTG AACATGAATT TGGCGCAACT ATGTTCCCTA 
CTCATGGCTT TGTGGGGGAA TTTCCTGGAA GTTGTGTCAC TAAATGAGCG TGTTTTTGTG 
CTTTTGGCCC AGGAGCACCA CACACTACGG GTGCAAAGAT TTGCTGAAGC TTTCTTTTGT 
CTTGAACACC CACGACAGAG TGCACTGGCC TACCAGGAAC TGCATGCTCA CAGTCACCAA 
CAAATGACCA CAGCTATCAA AAGCTCTTCC TACTTCTTGT CCTTACCACC TCTTCCTGTT 
GAATGTGCTG ATCTTGACGG GGATGTGAAC TCCCTACCAA TCATCTTTGA GGACAGATAT 
TTGGAGTCTG TTACTGAAGA TCGTGATGGT CCATGGCTTG GACTCCAAAA CAACAGGAGT 
GGCTCTGTGT CCGCCAAAGC AGATAGATCC AACTCAAAGG ACTGCATTGC CAGTGGAGCC 
CTCGGTCCGT ATCCAGATAA GCAGCAAGCT TCCTTTGCCA CTGCTTGGCC TGAACAAAAC 
GAGCCTTCCC TTAAATCCAA AGCCAAGTCC ATCAAGCTCA AGAAGAACAC CAAGACCGAA 
AACTCAAAGA AGCTGATCAG ACAGAGCTCG AAAGATTCTG TGGTGCTCAC AGGCTACAAG 
AACTTGAAGG TTCCTCACAG TGAAAAGACC ATTAAGTCCA AAGACATCGA CCATCAATGC 
AATCAGAGAG ATGAGCTAGA GGATGGAGGT AAATTCCTTC TTCAGAATGA AACCACGACC 
ACGCATTGTG CTCAGAATGC TAAAGACTGC ACATCTGGCA ACCTTCAAGG TAACGCCACA 
ATCCTAAGTG ACGGCTTTCC CAATCTTATG AGTTTGCAAA TGCAGGCCAG GCCCCAGGCA 
GGTACTGGAG CAGAGGCCGC CGCTGCCAGA GTAGATCCCA CACATCCACC TGCTGGCCTC 
AGACATATTG ATGTCAAACC CAGTGACAAG GACCCCTACC AGGGCGATAA GGTCACCGTT 
CTCTTACCGC ACCACCCAGA GCGCACCACC AAGAGGGATG TTCCAGAAAT CACACAGACT 
GATTTCTCTG AGTTCAAGAG TCGAGTTGGA GATGGTGCTA CTTTCGTCGG ACCTTGTGGA 
GCGGCTTTGA GGAACGATAA TATTCCTCAA GCCTCCTCCA GACTCTCGGA TTCCGGAATT 
GAAAGCGAAC CCAGCTCTGC GACACAGCTA GCCCCGGTTT CTCCATCTCC TTTTGCTCCT 
ATTGGGATCG AAGCACCTAC AGAGCTGATT CCTCACAGAC CACCTCCGCT CAGTCCTGCT 
TCCAGAGGAG CCACCGCATT GGAAGGCCTC AATCCTGAAA GCACCAGCGG CGTAAGCGGC 
GTCCAATCTT CCCTCACCTC CATCAACTCA CTCCCTTCGG ATGATGAGGT GGAGACAGAG 
GCCACCAGCA GGAAGTCCAG CATCCTGGTC CAGGAACAGA GTCTCATCTT CTCCGGAGAA 
TCTCAAAGCC TCTTGCTGAC AAACTCTAGA GTCTTGGCTC AACAAAAAGC CAGTCATGGA 
GATGGGATGG ACTCTGCTTT GCAGTCCCGC TGTGAAACTT CTGCTTCTGA CAGACTTGTC 
GGATCTAACG AGACAGATCT TCCAGGTGGT CCGGAAGCTT GTAGTGAACC ACAGACAGCA 
GCCCAGTCCA GTACATCAGT CACCAGCAGC ACAGACATGG TGAAGAAAGG CTTTGTAGAA 
AACTACTTTG GCTCTCGGTC CAGTACTGAT ATATCTGAAA TCAGCCCTTT AGAGACGGCA 
GCCATAACAT TGGGTATTCA GACATGCCCC AATGCTAATG ATGAAGAGGA TGAGGAGGAA 
GAGCAGGAGC ATGAGATGAT TGAGAATGGT TATTATGAGG AGACCGATGG TGTGGTTTAT 
GTTAATGGGC TCCCGGAGGA TGAAGGGAGG TGTGAAGATG GAGGAGCGGA GGGAAGAACA 
CTGTTTTATG ATCATCTTGG TTTAGAGCAG CTCCAGGAAA CTTACATTCC CTCTGCCGGC 
ATTCGGACAC GGCCTACTGG ACCAGCACCA ACCAGCAGCG ACTTATGCTG GTACAAGAGC 
GCCCCCACTG CTCAGATGAC GGCTTTTGTC CAAGCGAAAG AGGAGTTGAA ACAACTAAAG 
CTGCCAGGCT TCCTGTACAG TGAGGTGGCA GATCTGGCAT CCACTGTGCC ATATTTCAGC 
ATGGATGAGG ATGAAAACTG TGATGATGGC ATACACCTTA TAGTGTGTGT ACACGGCTTG 
GATGGTAACA GTGCAGATTT GCGCCTGGTG AAGACTTATC TGGAGCTTGG GTTGCCTGGA 
GCTAGGATAG ATTTTCTGAT GTCTGAGCGA AATCAGAATG ACACATTTGC TGATTTTGAG 
TCCATGACCG ACAGACTTTT GGATGAAATT GTGCAGTACA TACAAATCTA CAACCTCACA 
GTTTCCAAGA TCAGTTTTGT TGGTCATTCT CTGGGAAATC TGATCGTGCG CTCTGTGCTG 
ACGAGGCCCA GGTTTAAGTG TTATCTCAGC CGACTGCACA CCTTTCTGTC TCTGTCTGGA 
CCTCACCTCG GCACACTGTA CAACAGCTCA GCACTCGTCA ATACTGGACT GTGGTTCATG 
CAGAAGTGGA AGAAGTCTGG CTCTCTGCTG CAGCTGACCT GTCGAGACCA TTCAGACCCG 
CGTCAGACCT TTCTCTATAA GCTGAGCAAG AAAGCAGGCC TTCAGTTCTT CAAGAATGTG 
GTGTTGGTGG GCTCTCTTCA GGACCGATAC GTTCCTTATC ACTCCGCTCG AATCGAGATG 
TGCAAAACAG CCTTAAAGGA CAAACAGACT GGTCCGGTGT ATGCTGAGAT GATCGAGAAC 
CTTCTCCTTC CTGTGCTTCA GAACAAAGAC TGTAACCTGG TGCGTTACGA CGTCATCCAC 
GCGCTGCCCA ACACCGCCAA CTCTCTGATC GGCCGCGCAG CTCACATCGC CGTCCTGGAC 
TCTGAGATTT TCCTGGAAAA GTTCTTCCTG GTGGCAGGGC TCAAGTTTTT TCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam135a ( XM_017358777.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214266.1 Danio rerio family with sequence similarity 135, member A (fam135a), transcript variant X6, mRNA.

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CloneID	ODa54723
Clone ID Related Accession (Same CDS sequence)	XM_017358777.2 , XM_017358777.1
Accession Version	XM_017358777.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein FAM135A isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358777.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGGT 
GTTTTCCTTC TGCAGGTAGA AGAGTCTTTA AATGAAGTTG ACTTTCAGCT GTGTTTGGAC 
CTGTTCTTCA CTGATGGAGA CTACTCGCCT GAGGAGCCCA GTTCTCTGCA GAGCATCAGC 
AGCCGTACGC TGCGTTTGCA CTTTAGCCTC CACAGAGGCA TCCACCACCA CGTCAACGTC 
ATGTTTGACT ACTTCCACCT GTCTGTCATC TCTGCCACAA TCCATGCTTC ACTGGTGGCT 
CTGCATCAGC CTCTAATAAG TTTCCCCCGG CCAGTAAAAA GCACCTGGTT GAATCGTAAT 
GCTCCGGCAC AGAGCAAAGA CTCCCTCATC CCACCGCTGG AGACTGTGGT GTTCGGCAGC 
ACCTACGTCA AGCAGCTGTC CCCTGATGGC AAAAGCTTCC TGGTATCGGA TCAGTGCCTC 
CAGCATGCCT TCAGCCTGCA CCAGAACATG TGTGCCAACC TGCTGCGGGC ATATCAGAGC 
CTGTTCTGTT ACTTAACCTC CCTGACCAAG AATCTGCCCG CATCCCAGCG CATGGAGCTT 
GAGGAACTTG ATGTGGAGGT CCGACTTCAT GAGCTTTGTG AACAAGTCAA GAAAGCAGAG 
AGCCCAGATG AGTTGGCTGA ATTGGTGAAC ATGAATTTGG CGCAACTATG TTCCCTACTC 
ATGGCTTTGT GGGGGAATTT CCTGGAAGTT GTGTCACTAA ATGAGCGTGT TTTTGTGCTT 
TTGGCCCAGG AGCACCACAC ACTACGGGTG CAAAGATTTG CTGAAGCTTT CTTTTGTCTT 
GAACACCCAC GACAGAGTGC ACTGGCCTAC CAGGAACTGC ATGCTCACAG TCACCAACAA 
ATGACCACAG CTATCAAAAG CTCTTCCTAC TTCTTGTCCT TACCACCTCT TCCTGTTGAA 
TGTGCTGATC TTGACGGGGA TGTGAACTCC CTACCAATCA TCTTTGAGGA CAGATATTTG 
GAGTCTGTTA CTGAAGATCG TGATGGTCCA TGGCTTGGAC TCCAAAACAA CAGGAGTGGC 
TCTGTGTCCG CCAAAGCAGA TAGATCCAAC TCAAAGGACT GCATTGCCAG TGGAGCCCTC 
GGTCCGTATC CAGATAAGCA GCAAGCTTCC TTTGCCACTG CTTGGCCTGA ACAAAACGAG 
CCTTCCCTTA AATCCAAAGC CAAGTCCATC AAGCTCAAGA AGAACACCAA GACCGAAAAC 
TCAAAGAAGC TGATCAGACA GAGCTCGAAA GATTCTGTGG TGCTCACAGG CTACAAGAAC 
TTGAAGGTTC CTCACAGTGA AAAGACCATT AAGTCCAAAG ACATCGACCA TCAATGCAAT 
CAGAGAGATG AGCTAGAGGA TGGAGGTAAA TTCCTTCTTC AGAATGAAAC CACGACCACG 
CATTGTGCTC AGAATGCTAA AGACTGCACA TCTGGCAACC TTCAAGGTAA CGCCACAATC 
CTAAGTGACG GCTTTCCCAA TCTTATGAGT TTGCAAATGC AGGCCAGGCC CCAGGCAGGT 
ACTGGAGCAG AGGCCGCCGC TGCCAGAGTA GATCCCACAC ATCCACCTGC TGGCCTCAGA 
CATATTGATG TCAAACCCAG TGACAAGGAC CCCTACCAGG GCGATAAGGT CACCGTTCTC 
TTACCGCACC ACCCAGAGCG CACCACCAAG AGGGATGTTC CAGAAATCAC ACAGACTGAT 
TTCTCTGAGT TCAAGAGTCG AGTTGGAGAT GGTGCTACTT TCGTCGGACC TTGTGGAGCG 
GCTTTGAGGA ACGATAATAT TCCTCAAGCC TCCTCCAGAC TCTCGGATTC CGGAATTGAA 
AGCGAACCCA GCTCTGCGAC ACAGCTAGCC CCGGTTTCTC CATCTCCTTT TGCTCCTATT 
GGGATCGAAG CACCTACAGA GCTGATTCCT CACAGACCAC CTCCGCTCAG TCCTGCTTCC 
AGAGGAGCCA CCGCATTGGA AGGCCTCAAT CCTGAAAGCA CCAGCGGCGT AAGCGGCGTC 
CAATCTTCCC TCACCTCCAT CAACTCACTC CCTTCGGATG ATGAGGTGGA GACAGAGGCC 
ACCAGCAGGA AGTCCAGCAT CCTGGTCCAG GAACAGAGTC TCATCTTCTC CGGAGAATCT 
CAAAGCCTCT TGCTGACAAA CTCTAGAGTC TTGGCTCAAC AAAAAGCCAG TCATGGAGAT 
GGGATGGACT CTGCTTTGCA GTCCCGCTGT GAAACTTCTG CTTCTGACAG ACTTGTCGGA 
TCTAACGAGA CAGATCTTCC AGGTGGTCCG GAAGCTTGTA GTGAACCACA GACAGCAGCC 
CAGTCCAGTA CATCAGTCAC CAGCAGCACA GACATGGTGA AGAAAGGCTT TGTAGAAAAC 
TACTTTGGCT CTCGGTCCAG TACTGATATA TCTGAAATCA GCCCTTTAGA GACGGCAGCC 
ATAACATTGG GTATTCAGAC ATGCCCCAAT GCTAATGATG AAGAGGATGA GGAGGAAGAG 
CAGGAGCATG AGATGATTGA GAATGGTTAT TATGAGGAGA CCGATGGTGT GGTTTATGTT 
AATGGGCTCC CGGAGGATGA AGGGAGGTGT GAAGATGGAG GAGCGGAGGG AAGAACACTG 
TTTTATGATC ATCTTGGTTT AGAGCAGCTC CAGGAAACTT ACATTCCCTC TGCCGGCATT 
CGGACACGGC CTACTGGACC AGCACCAACC AGCAGCGACT TATGCTGGTA CAAGAGCGCC 
CCCACTGCTC AGATGACGGC TTTTGTCCAA GCGAAAGAGG AGTTGAAACA ACTAAAGCTG 
CCAGGCTTCC TGTACAGTGA GGTGGCAGAT CTGGCATCCA CTGTGCCATA TTTCAGCATG 
GATGAGGATG AAAACTGTGA TGATGGCATA CACCTTATAG TGTGTGTACA CGGCTTGGAT 
GGTAACAGTG CAGATTTGCG CCTGGTGAAG ACTTATCTGG AGCTTGGGTT GCCTGGAGCT 
AGGATAGATT TTCTGATGTC TGAGCGAAAT CAGAATGACA CATTTGCTGA TTTTGAGTCC 
ATGACCGACA GACTTTTGGA TGAAATTGTG CAGTACATAC AAATCTACAA CCTCACAGTT 
TCCAAGATCA GTTTTGTTGG TCATTCTCTG GGAAATCTGA TCGTGCGCTC TGTGCTGACG 
AGGCCCAGGT TTAAGTGTTA TCTCAGCCGA CTGCACACCT TTCTGTCTCT GTCTGGACCT 
CACCTCGGCA CACTGTACAA CAGCTCAGCA CTCGTCAATA CTGGACTGTG GTTCATGCAG 
AAGTGGAAGA AGTCTGGCTC TCTGCTGCAG CTGACCTGTC GAGACCATTC AGACCCGCGT 
CAGACCTTTC TCTATAAGCT GAGCAAGAAA GCAGGCCTTC AGTTCTTCAA GAATGTGGTG 
TTGGTGGGCT CTCTTCAGGA CCGATACGTT CCTTATCACT CCGCTCGAAT CGAGATGTGC 
AAAACAGCCT TAAAGGACAA ACAGACTGGT CCGGTGTATG CTGAGATGAT CGAGAACCTT 
CTCCTTCCTG TGCTTCAGAA CAAAGACTGT AACCTGGTGC GTTACGACGT CATCCACGCG 
CTGCCCAACA CCGCCAACTC TCTGATCGGC CGCGCAGCTC ACATCGCCGT CCTGGACTCT 
GAGATTTTCC TGGAAAAGTT CTTCCTGGTG GCAGGGCTCA AGTTTTTTCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214266.1
CDS	405..4358
Translation	MTEVQATVEFSVELHKFYNVDLFQRGFYQVRASMKVPPRIPQRVEASLLHPIGSDLAFPASVQDDVVCSKTFQILYKNEEIVVNDVFVFKVMMLLDAKKGVFLLQVEESLNEVDFQLCLDLFFTDGDYSPEEPSSLQSISSRTLRLHFSLHRGIHHHVNVMFDYFHLSVISATIHASLVALHQPLISFPRPVKSTWLNRNAPAQSKDSLIPPLETVVFGSTYVKQLSPDGKSFLVSDQCLQHAFSLHQNMCANLLRAYQSLFCYLTSLTKNLPASQRMELEELDVEVRLHELCEQVKKAESPDELAELVNMNLAQLCSLLMALWGNFLEVVSLNERVFVLLAQEHHTLRVQRFAEAFFCLEHPRQSALAYQELHAHSHQQMTTAIKSSSYFLSLPPLPVECADLDGDVNSLPIIFEDRYLESVTEDRDGPWLGLQNNRSGSVSAKADRSNSKDCIASGALGPYPDKQQASFATAWPEQNEPSLKSKAKSIKLKKNTKTENSKKLIRQSSKDSVVLTGYKNLKVPHSEKTIKSKDIDHQCNQRDELEDGGKFLLQNETTTTHCAQNAKDCTSGNLQGNATILSDGFPNLMSLQMQARPQAGTGAEAAAARVDPTHPPAGLRHIDVKPSDKDPYQGDKVTVLLPHHPERTTKRDVPEITQTDFSEFKSRVGDGATFVGPCGAALRNDNIPQASSRLSDSGIESEPSSATQLAPVSPSPFAPIGIEAPTELIPHRPPPLSPASRGATALEGLNPESTSGVSGVQSSLTSINSLPSDDEVETEATSRKSSILVQEQSLIFSGESQSLLLTNSRVLAQQKASHGDGMDSALQSRCETSASDRLVGSNETDLPGGPEACSEPQTAAQSSTSVTSSTDMVKKGFVENYFGSRSSTDISEISPLETAAITLGIQTCPNANDEEDEEEEQEHEMIENGYYEETDGVVYVNGLPEDEGRCEDGGAEGRTLFYDHLGLEQLQETYIPSAGIRTRPTGPAPTSSDLCWYKSAPTAQMTAFVQAKEELKQLKLPGFLYSEVADLASTVPYFSMDEDENCDDGIHLIVCVHGLDGNSADLRLVKTYLELGLPGARIDFLMSERNQNDTFADFESMTDRLLDEIVQYIQIYNLTVSKISFVGHSLGNLIVRSVLTRPRFKCYLSRLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSKKAGLQFFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQTGPVYAEMIENLLLPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEIFLEKFFLVAGLKFFQ

Target ORF information:

RefSeq Version	XM_017358777.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio family with sequence similarity 135, member A (fam135a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358777.2

ORF Insert Sequence:

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGGT 
GTTTTCCTTC TGCAGGTAGA AGAGTCTTTA AATGAAGTTG ACTTTCAGCT GTGTTTGGAC 
CTGTTCTTCA CTGATGGAGA CTACTCGCCT GAGGAGCCCA GTTCTCTGCA GAGCATCAGC 
AGCCGTACGC TGCGTTTGCA CTTTAGCCTC CACAGAGGCA TCCACCACCA CGTCAACGTC 
ATGTTTGACT ACTTCCACCT GTCTGTCATC TCTGCCACAA TCCATGCTTC ACTGGTGGCT 
CTGCATCAGC CTCTAATAAG TTTCCCCCGG CCAGTAAAAA GCACCTGGTT GAATCGTAAT 
GCTCCGGCAC AGAGCAAAGA CTCCCTCATC CCACCGCTGG AGACTGTGGT GTTCGGCAGC 
ACCTACGTCA AGCAGCTGTC CCCTGATGGC AAAAGCTTCC TGGTATCGGA TCAGTGCCTC 
CAGCATGCCT TCAGCCTGCA CCAGAACATG TGTGCCAACC TGCTGCGGGC ATATCAGAGC 
CTGTTCTGTT ACTTAACCTC CCTGACCAAG AATCTGCCCG CATCCCAGCG CATGGAGCTT 
GAGGAACTTG ATGTGGAGGT CCGACTTCAT GAGCTTTGTG AACAAGTCAA GAAAGCAGAG 
AGCCCAGATG AGTTGGCTGA ATTGGTGAAC ATGAATTTGG CGCAACTATG TTCCCTACTC 
ATGGCTTTGT GGGGGAATTT CCTGGAAGTT GTGTCACTAA ATGAGCGTGT TTTTGTGCTT 
TTGGCCCAGG AGCACCACAC ACTACGGGTG CAAAGATTTG CTGAAGCTTT CTTTTGTCTT 
GAACACCCAC GACAGAGTGC ACTGGCCTAC CAGGAACTGC ATGCTCACAG TCACCAACAA 
ATGACCACAG CTATCAAAAG CTCTTCCTAC TTCTTGTCCT TACCACCTCT TCCTGTTGAA 
TGTGCTGATC TTGACGGGGA TGTGAACTCC CTACCAATCA TCTTTGAGGA CAGATATTTG 
GAGTCTGTTA CTGAAGATCG TGATGGTCCA TGGCTTGGAC TCCAAAACAA CAGGAGTGGC 
TCTGTGTCCG CCAAAGCAGA TAGATCCAAC TCAAAGGACT GCATTGCCAG TGGAGCCCTC 
GGTCCGTATC CAGATAAGCA GCAAGCTTCC TTTGCCACTG CTTGGCCTGA ACAAAACGAG 
CCTTCCCTTA AATCCAAAGC CAAGTCCATC AAGCTCAAGA AGAACACCAA GACCGAAAAC 
TCAAAGAAGC TGATCAGACA GAGCTCGAAA GATTCTGTGG TGCTCACAGG CTACAAGAAC 
TTGAAGGTTC CTCACAGTGA AAAGACCATT AAGTCCAAAG ACATCGACCA TCAATGCAAT 
CAGAGAGATG AGCTAGAGGA TGGAGGTAAA TTCCTTCTTC AGAATGAAAC CACGACCACG 
CATTGTGCTC AGAATGCTAA AGACTGCACA TCTGGCAACC TTCAAGGTAA CGCCACAATC 
CTAAGTGACG GCTTTCCCAA TCTTATGAGT TTGCAAATGC AGGCCAGGCC CCAGGCAGGT 
ACTGGAGCAG AGGCCGCCGC TGCCAGAGTA GATCCCACAC ATCCACCTGC TGGCCTCAGA 
CATATTGATG TCAAACCCAG TGACAAGGAC CCCTACCAGG GCGATAAGGT CACCGTTCTC 
TTACCGCACC ACCCAGAGCG CACCACCAAG AGGGATGTTC CAGAAATCAC ACAGACTGAT 
TTCTCTGAGT TCAAGAGTCG AGTTGGAGAT GGTGCTACTT TCGTCGGACC TTGTGGAGCG 
GCTTTGAGGA ACGATAATAT TCCTCAAGCC TCCTCCAGAC TCTCGGATTC CGGAATTGAA 
AGCGAACCCA GCTCTGCGAC ACAGCTAGCC CCGGTTTCTC CATCTCCTTT TGCTCCTATT 
GGGATCGAAG CACCTACAGA GCTGATTCCT CACAGACCAC CTCCGCTCAG TCCTGCTTCC 
AGAGGAGCCA CCGCATTGGA AGGCCTCAAT CCTGAAAGCA CCAGCGGCGT AAGCGGCGTC 
CAATCTTCCC TCACCTCCAT CAACTCACTC CCTTCGGATG ATGAGGTGGA GACAGAGGCC 
ACCAGCAGGA AGTCCAGCAT CCTGGTCCAG GAACAGAGTC TCATCTTCTC CGGAGAATCT 
CAAAGCCTCT TGCTGACAAA CTCTAGAGTC TTGGCTCAAC AAAAAGCCAG TCATGGAGAT 
GGGATGGACT CTGCTTTGCA GTCCCGCTGT GAAACTTCTG CTTCTGACAG ACTTGTCGGA 
TCTAACGAGA CAGATCTTCC AGGTGGTCCG GAAGCTTGTA GTGAACCACA GACAGCAGCC 
CAGTCCAGTA CATCAGTCAC CAGCAGCACA GACATGGTGA AGAAAGGCTT TGTAGAAAAC 
TACTTTGGCT CTCGGTCCAG TACTGATATA TCTGAAATCA GCCCTTTAGA GACGGCAGCC 
ATAACATTGG GTATTCAGAC ATGCCCCAAT GCTAATGATG AAGAGGATGA GGAGGAAGAG 
CAGGAGCATG AGATGATTGA GAATGGTTAT TATGAGGAGA CCGATGGTGT GGTTTATGTT 
AATGGGCTCC CGGAGGATGA AGGGAGGTGT GAAGATGGAG GAGCGGAGGG AAGAACACTG 
TTTTATGATC ATCTTGGTTT AGAGCAGCTC CAGGAAACTT ACATTCCCTC TGCCGGCATT 
CGGACACGGC CTACTGGACC AGCACCAACC AGCAGCGACT TATGCTGGTA CAAGAGCGCC 
CCCACTGCTC AGATGACGGC TTTTGTCCAA GCGAAAGAGG AGTTGAAACA ACTAAAGCTG 
CCAGGCTTCC TGTACAGTGA GGTGGCAGAT CTGGCATCCA CTGTGCCATA TTTCAGCATG 
GATGAGGATG AAAACTGTGA TGATGGCATA CACCTTATAG TGTGTGTACA CGGCTTGGAT 
GGTAACAGTG CAGATTTGCG CCTGGTGAAG ACTTATCTGG AGCTTGGGTT GCCTGGAGCT 
AGGATAGATT TTCTGATGTC TGAGCGAAAT CAGAATGACA CATTTGCTGA TTTTGAGTCC 
ATGACCGACA GACTTTTGGA TGAAATTGTG CAGTACATAC AAATCTACAA CCTCACAGTT 
TCCAAGATCA GTTTTGTTGG TCATTCTCTG GGAAATCTGA TCGTGCGCTC TGTGCTGACG 
AGGCCCAGGT TTAAGTGTTA TCTCAGCCGA CTGCACACCT TTCTGTCTCT GTCTGGACCT 
CACCTCGGCA CACTGTACAA CAGCTCAGCA CTCGTCAATA CTGGACTGTG GTTCATGCAG 
AAGTGGAAGA AGTCTGGCTC TCTGCTGCAG CTGACCTGTC GAGACCATTC AGACCCGCGT 
CAGACCTTTC TCTATAAGCT GAGCAAGAAA GCAGGCCTTC AGTTCTTCAA GAATGTGGTG 
TTGGTGGGCT CTCTTCAGGA CCGATACGTT CCTTATCACT CCGCTCGAAT CGAGATGTGC 
AAAACAGCCT TAAAGGACAA ACAGACTGGT CCGGTGTATG CTGAGATGAT CGAGAACCTT 
CTCCTTCCTG TGCTTCAGAA CAAAGACTGT AACCTGGTGC GTTACGACGT CATCCACGCG 
CTGCCCAACA CCGCCAACTC TCTGATCGGC CGCGCAGCTC ACATCGCCGT CCTGGACTCT 
GAGATTTTCC TGGAAAAGTT CTTCCTGGTG GCAGGGCTCA AGTTTTTTCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam135a ( XM_017358777.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017214266.1 Danio rerio family with sequence similarity 135 member A (fam135a), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa54723
Clone ID Related Accession (Same CDS sequence)	XM_017358777.2 , XM_017358777.1
Accession Version	XM_017358777.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein FAM135A isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGGT 
GTTTTCCTTC TGCAGGTAGA AGAGTCTTTA AATGAAGTTG ACTTTCAGCT GTGTTTGGAC 
CTGTTCTTCA CTGATGGAGA CTACTCGCCT GAGGAGCCCA GTTCTCTGCA GAGCATCAGC 
AGCCGTACGC TGCGTTTGCA CTTTAGCCTC CACAGAGGCA TCCACCACCA CGTCAACGTC 
ATGTTTGACT ACTTCCACCT GTCTGTCATC TCTGCCACAA TCCATGCTTC ACTGGTGGCT 
CTGCATCAGC CTCTAATAAG TTTCCCCCGG CCAGTAAAAA GCACCTGGTT GAATCGTAAT 
GCTCCGGCAC AGAGCAAAGA CTCCCTCATC CCACCGCTGG AGACTGTGGT GTTCGGCAGC 
ACCTACGTCA AGCAGCTGTC CCCTGATGGC AAAAGCTTCC TGGTATCGGA TCAGTGCCTC 
CAGCATGCCT TCAGCCTGCA CCAGAACATG TGTGCCAACC TGCTGCGGGC ATATCAGAGC 
CTGTTCTGTT ACTTAACCTC CCTGACCAAG AATCTGCCCG CATCCCAGCG CATGGAGCTT 
GAGGAACTTG ATGTGGAGGT CCGACTTCAT GAGCTTTGTG AACAAGTCAA GAAAGCAGAG 
AGCCCAGATG AGTTGGCTGA ATTGGTGAAC ATGAATTTGG CGCAACTATG TTCCCTACTC 
ATGGCTTTGT GGGGGAATTT CCTGGAAGTT GTGTCACTAA ATGAGCGTGT TTTTGTGCTT 
TTGGCCCAGG AGCACCACAC ACTACGGGTG CAAAGATTTG CTGAAGCTTT CTTTTGTCTT 
GAACACCCAC GACAGAGTGC ACTGGCCTAC CAGGAACTGC ATGCTCACAG TCACCAACAA 
ATGACCACAG CTATCAAAAG CTCTTCCTAC TTCTTGTCCT TACCACCTCT TCCTGTTGAA 
TGTGCTGATC TTGACGGGGA TGTGAACTCC CTACCAATCA TCTTTGAGGA CAGATATTTG 
GAGTCTGTTA CTGAAGATCG TGATGGTCCA TGGCTTGGAC TCCAAAACAA CAGGAGTGGC 
TCTGTGTCCG CCAAAGCAGA TAGATCCAAC TCAAAGGACT GCATTGCCAG TGGAGCCCTC 
GGTCCGTATC CAGATAAGCA GCAAGCTTCC TTTGCCACTG CTTGGCCTGA ACAAAACGAG 
CCTTCCCTTA AATCCAAAGC CAAGTCCATC AAGCTCAAGA AGAACACCAA GACCGAAAAC 
TCAAAGAAGC TGATCAGACA GAGCTCGAAA GATTCTGTGG TGCTCACAGG CTACAAGAAC 
TTGAAGGTTC CTCACAGTGA AAAGACCATT AAGTCCAAAG ACATCGACCA TCAATGCAAT 
CAGAGAGATG AGCTAGAGGA TGGAGGTAAA TTCCTTCTTC AGAATGAAAC CACGACCACG 
CATTGTGCTC AGAATGCTAA AGACTGCACA TCTGGCAACC TTCAAGGTAA CGCCACAATC 
CTAAGTGACG GCTTTCCCAA TCTTATGAGT TTGCAAATGC AGGCCAGGCC CCAGGCAGGT 
ACTGGAGCAG AGGCCGCCGC TGCCAGAGTA GATCCCACAC ATCCACCTGC TGGCCTCAGA 
CATATTGATG TCAAACCCAG TGACAAGGAC CCCTACCAGG GCGATAAGGT CACCGTTCTC 
TTACCGCACC ACCCAGAGCG CACCACCAAG AGGGATGTTC CAGAAATCAC ACAGACTGAT 
TTCTCTGAGT TCAAGAGTCG AGTTGGAGAT GGTGCTACTT TCGTCGGACC TTGTGGAGCG 
GCTTTGAGGA ACGATAATAT TCCTCAAGCC TCCTCCAGAC TCTCGGATTC CGGAATTGAA 
AGCGAACCCA GCTCTGCGAC ACAGCTAGCC CCGGTTTCTC CATCTCCTTT TGCTCCTATT 
GGGATCGAAG CACCTACAGA GCTGATTCCT CACAGACCAC CTCCGCTCAG TCCTGCTTCC 
AGAGGAGCCA CCGCATTGGA AGGCCTCAAT CCTGAAAGCA CCAGCGGCGT AAGCGGCGTC 
CAATCTTCCC TCACCTCCAT CAACTCACTC CCTTCGGATG ATGAGGTGGA GACAGAGGCC 
ACCAGCAGGA AGTCCAGCAT CCTGGTCCAG GAACAGAGTC TCATCTTCTC CGGAGAATCT 
CAAAGCCTCT TGCTGACAAA CTCTAGAGTC TTGGCTCAAC AAAAAGCCAG TCATGGAGAT 
GGGATGGACT CTGCTTTGCA GTCCCGCTGT GAAACTTCTG CTTCTGACAG ACTTGTCGGA 
TCTAACGAGA CAGATCTTCC AGGTGGTCCG GAAGCTTGTA GTGAACCACA GACAGCAGCC 
CAGTCCAGTA CATCAGTCAC CAGCAGCACA GACATGGTGA AGAAAGGCTT TGTAGAAAAC 
TACTTTGGCT CTCGGTCCAG TACTGATATA TCTGAAATCA GCCCTTTAGA GACGGCAGCC 
ATAACATTGG GTATTCAGAC ATGCCCCAAT GCTAATGATG AAGAGGATGA GGAGGAAGAG 
CAGGAGCATG AGATGATTGA GAATGGTTAT TATGAGGAGA CCGATGGTGT GGTTTATGTT 
AATGGGCTCC CGGAGGATGA AGGGAGGTGT GAAGATGGAG GAGCGGAGGG AAGAACACTG 
TTTTATGATC ATCTTGGTTT AGAGCAGCTC CAGGAAACTT ACATTCCCTC TGCCGGCATT 
CGGACACGGC CTACTGGACC AGCACCAACC AGCAGCGACT TATGCTGGTA CAAGAGCGCC 
CCCACTGCTC AGATGACGGC TTTTGTCCAA GCGAAAGAGG AGTTGAAACA ACTAAAGCTG 
CCAGGCTTCC TGTACAGTGA GGTGGCAGAT CTGGCATCCA CTGTGCCATA TTTCAGCATG 
GATGAGGATG AAAACTGTGA TGATGGCATA CACCTTATAG TGTGTGTACA CGGCTTGGAT 
GGTAACAGTG CAGATTTGCG CCTGGTGAAG ACTTATCTGG AGCTTGGGTT GCCTGGAGCT 
AGGATAGATT TTCTGATGTC TGAGCGAAAT CAGAATGACA CATTTGCTGA TTTTGAGTCC 
ATGACCGACA GACTTTTGGA TGAAATTGTG CAGTACATAC AAATCTACAA CCTCACAGTT 
TCCAAGATCA GTTTTGTTGG TCATTCTCTG GGAAATCTGA TCGTGCGCTC TGTGCTGACG 
AGGCCCAGGT TTAAGTGTTA TCTCAGCCGA CTGCACACCT TTCTGTCTCT GTCTGGACCT 
CACCTCGGCA CACTGTACAA CAGCTCAGCA CTCGTCAATA CTGGACTGTG GTTCATGCAG 
AAGTGGAAGA AGTCTGGCTC TCTGCTGCAG CTGACCTGTC GAGACCATTC AGACCCGCGT 
CAGACCTTTC TCTATAAGCT GAGCAAGAAA GCAGGCCTTC AGTTCTTCAA GAATGTGGTG 
TTGGTGGGCT CTCTTCAGGA CCGATACGTT CCTTATCACT CCGCTCGAAT CGAGATGTGC 
AAAACAGCCT TAAAGGACAA ACAGACTGGT CCGGTGTATG CTGAGATGAT CGAGAACCTT 
CTCCTTCCTG TGCTTCAGAA CAAAGACTGT AACCTGGTGC GTTACGACGT CATCCACGCG 
CTGCCCAACA CCGCCAACTC TCTGATCGGC CGCGCAGCTC ACATCGCCGT CCTGGACTCT 
GAGATTTTCC TGGAAAAGTT CTTCCTGGTG GCAGGGCTCA AGTTTTTTCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017214266.1
CDS	388..4341
Translation	MTEVQATVEFSVELHKFYNVDLFQRGFYQVRASMKVPPRIPQRVEASLLHPIGSDLAFPASVQDDVVCSKTFQILYKNEEIVVNDVFVFKVMMLLDAKKGVFLLQVEESLNEVDFQLCLDLFFTDGDYSPEEPSSLQSISSRTLRLHFSLHRGIHHHVNVMFDYFHLSVISATIHASLVALHQPLISFPRPVKSTWLNRNAPAQSKDSLIPPLETVVFGSTYVKQLSPDGKSFLVSDQCLQHAFSLHQNMCANLLRAYQSLFCYLTSLTKNLPASQRMELEELDVEVRLHELCEQVKKAESPDELAELVNMNLAQLCSLLMALWGNFLEVVSLNERVFVLLAQEHHTLRVQRFAEAFFCLEHPRQSALAYQELHAHSHQQMTTAIKSSSYFLSLPPLPVECADLDGDVNSLPIIFEDRYLESVTEDRDGPWLGLQNNRSGSVSAKADRSNSKDCIASGALGPYPDKQQASFATAWPEQNEPSLKSKAKSIKLKKNTKTENSKKLIRQSSKDSVVLTGYKNLKVPHSEKTIKSKDIDHQCNQRDELEDGGKFLLQNETTTTHCAQNAKDCTSGNLQGNATILSDGFPNLMSLQMQARPQAGTGAEAAAARVDPTHPPAGLRHIDVKPSDKDPYQGDKVTVLLPHHPERTTKRDVPEITQTDFSEFKSRVGDGATFVGPCGAALRNDNIPQASSRLSDSGIESEPSSATQLAPVSPSPFAPIGIEAPTELIPHRPPPLSPASRGATALEGLNPESTSGVSGVQSSLTSINSLPSDDEVETEATSRKSSILVQEQSLIFSGESQSLLLTNSRVLAQQKASHGDGMDSALQSRCETSASDRLVGSNETDLPGGPEACSEPQTAAQSSTSVTSSTDMVKKGFVENYFGSRSSTDISEISPLETAAITLGIQTCPNANDEEDEEEEQEHEMIENGYYEETDGVVYVNGLPEDEGRCEDGGAEGRTLFYDHLGLEQLQETYIPSAGIRTRPTGPAPTSSDLCWYKSAPTAQMTAFVQAKEELKQLKLPGFLYSEVADLASTVPYFSMDEDENCDDGIHLIVCVHGLDGNSADLRLVKTYLELGLPGARIDFLMSERNQNDTFADFESMTDRLLDEIVQYIQIYNLTVSKISFVGHSLGNLIVRSVLTRPRFKCYLSRLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSKKAGLQFFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQTGPVYAEMIENLLLPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEIFLEKFFLVAGLKFFQ

Target ORF information:

RefSeq Version	XM_017358777.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio family with sequence similarity 135 member A (fam135a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358777.1

ORF Insert Sequence:

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGGT 
GTTTTCCTTC TGCAGGTAGA AGAGTCTTTA AATGAAGTTG ACTTTCAGCT GTGTTTGGAC 
CTGTTCTTCA CTGATGGAGA CTACTCGCCT GAGGAGCCCA GTTCTCTGCA GAGCATCAGC 
AGCCGTACGC TGCGTTTGCA CTTTAGCCTC CACAGAGGCA TCCACCACCA CGTCAACGTC 
ATGTTTGACT ACTTCCACCT GTCTGTCATC TCTGCCACAA TCCATGCTTC ACTGGTGGCT 
CTGCATCAGC CTCTAATAAG TTTCCCCCGG CCAGTAAAAA GCACCTGGTT GAATCGTAAT 
GCTCCGGCAC AGAGCAAAGA CTCCCTCATC CCACCGCTGG AGACTGTGGT GTTCGGCAGC 
ACCTACGTCA AGCAGCTGTC CCCTGATGGC AAAAGCTTCC TGGTATCGGA TCAGTGCCTC 
CAGCATGCCT TCAGCCTGCA CCAGAACATG TGTGCCAACC TGCTGCGGGC ATATCAGAGC 
CTGTTCTGTT ACTTAACCTC CCTGACCAAG AATCTGCCCG CATCCCAGCG CATGGAGCTT 
GAGGAACTTG ATGTGGAGGT CCGACTTCAT GAGCTTTGTG AACAAGTCAA GAAAGCAGAG 
AGCCCAGATG AGTTGGCTGA ATTGGTGAAC ATGAATTTGG CGCAACTATG TTCCCTACTC 
ATGGCTTTGT GGGGGAATTT CCTGGAAGTT GTGTCACTAA ATGAGCGTGT TTTTGTGCTT 
TTGGCCCAGG AGCACCACAC ACTACGGGTG CAAAGATTTG CTGAAGCTTT CTTTTGTCTT 
GAACACCCAC GACAGAGTGC ACTGGCCTAC CAGGAACTGC ATGCTCACAG TCACCAACAA 
ATGACCACAG CTATCAAAAG CTCTTCCTAC TTCTTGTCCT TACCACCTCT TCCTGTTGAA 
TGTGCTGATC TTGACGGGGA TGTGAACTCC CTACCAATCA TCTTTGAGGA CAGATATTTG 
GAGTCTGTTA CTGAAGATCG TGATGGTCCA TGGCTTGGAC TCCAAAACAA CAGGAGTGGC 
TCTGTGTCCG CCAAAGCAGA TAGATCCAAC TCAAAGGACT GCATTGCCAG TGGAGCCCTC 
GGTCCGTATC CAGATAAGCA GCAAGCTTCC TTTGCCACTG CTTGGCCTGA ACAAAACGAG 
CCTTCCCTTA AATCCAAAGC CAAGTCCATC AAGCTCAAGA AGAACACCAA GACCGAAAAC 
TCAAAGAAGC TGATCAGACA GAGCTCGAAA GATTCTGTGG TGCTCACAGG CTACAAGAAC 
TTGAAGGTTC CTCACAGTGA AAAGACCATT AAGTCCAAAG ACATCGACCA TCAATGCAAT 
CAGAGAGATG AGCTAGAGGA TGGAGGTAAA TTCCTTCTTC AGAATGAAAC CACGACCACG 
CATTGTGCTC AGAATGCTAA AGACTGCACA TCTGGCAACC TTCAAGGTAA CGCCACAATC 
CTAAGTGACG GCTTTCCCAA TCTTATGAGT TTGCAAATGC AGGCCAGGCC CCAGGCAGGT 
ACTGGAGCAG AGGCCGCCGC TGCCAGAGTA GATCCCACAC ATCCACCTGC TGGCCTCAGA 
CATATTGATG TCAAACCCAG TGACAAGGAC CCCTACCAGG GCGATAAGGT CACCGTTCTC 
TTACCGCACC ACCCAGAGCG CACCACCAAG AGGGATGTTC CAGAAATCAC ACAGACTGAT 
TTCTCTGAGT TCAAGAGTCG AGTTGGAGAT GGTGCTACTT TCGTCGGACC TTGTGGAGCG 
GCTTTGAGGA ACGATAATAT TCCTCAAGCC TCCTCCAGAC TCTCGGATTC CGGAATTGAA 
AGCGAACCCA GCTCTGCGAC ACAGCTAGCC CCGGTTTCTC CATCTCCTTT TGCTCCTATT 
GGGATCGAAG CACCTACAGA GCTGATTCCT CACAGACCAC CTCCGCTCAG TCCTGCTTCC 
AGAGGAGCCA CCGCATTGGA AGGCCTCAAT CCTGAAAGCA CCAGCGGCGT AAGCGGCGTC 
CAATCTTCCC TCACCTCCAT CAACTCACTC CCTTCGGATG ATGAGGTGGA GACAGAGGCC 
ACCAGCAGGA AGTCCAGCAT CCTGGTCCAG GAACAGAGTC TCATCTTCTC CGGAGAATCT 
CAAAGCCTCT TGCTGACAAA CTCTAGAGTC TTGGCTCAAC AAAAAGCCAG TCATGGAGAT 
GGGATGGACT CTGCTTTGCA GTCCCGCTGT GAAACTTCTG CTTCTGACAG ACTTGTCGGA 
TCTAACGAGA CAGATCTTCC AGGTGGTCCG GAAGCTTGTA GTGAACCACA GACAGCAGCC 
CAGTCCAGTA CATCAGTCAC CAGCAGCACA GACATGGTGA AGAAAGGCTT TGTAGAAAAC 
TACTTTGGCT CTCGGTCCAG TACTGATATA TCTGAAATCA GCCCTTTAGA GACGGCAGCC 
ATAACATTGG GTATTCAGAC ATGCCCCAAT GCTAATGATG AAGAGGATGA GGAGGAAGAG 
CAGGAGCATG AGATGATTGA GAATGGTTAT TATGAGGAGA CCGATGGTGT GGTTTATGTT 
AATGGGCTCC CGGAGGATGA AGGGAGGTGT GAAGATGGAG GAGCGGAGGG AAGAACACTG 
TTTTATGATC ATCTTGGTTT AGAGCAGCTC CAGGAAACTT ACATTCCCTC TGCCGGCATT 
CGGACACGGC CTACTGGACC AGCACCAACC AGCAGCGACT TATGCTGGTA CAAGAGCGCC 
CCCACTGCTC AGATGACGGC TTTTGTCCAA GCGAAAGAGG AGTTGAAACA ACTAAAGCTG 
CCAGGCTTCC TGTACAGTGA GGTGGCAGAT CTGGCATCCA CTGTGCCATA TTTCAGCATG 
GATGAGGATG AAAACTGTGA TGATGGCATA CACCTTATAG TGTGTGTACA CGGCTTGGAT 
GGTAACAGTG CAGATTTGCG CCTGGTGAAG ACTTATCTGG AGCTTGGGTT GCCTGGAGCT 
AGGATAGATT TTCTGATGTC TGAGCGAAAT CAGAATGACA CATTTGCTGA TTTTGAGTCC 
ATGACCGACA GACTTTTGGA TGAAATTGTG CAGTACATAC AAATCTACAA CCTCACAGTT 
TCCAAGATCA GTTTTGTTGG TCATTCTCTG GGAAATCTGA TCGTGCGCTC TGTGCTGACG 
AGGCCCAGGT TTAAGTGTTA TCTCAGCCGA CTGCACACCT TTCTGTCTCT GTCTGGACCT 
CACCTCGGCA CACTGTACAA CAGCTCAGCA CTCGTCAATA CTGGACTGTG GTTCATGCAG 
AAGTGGAAGA AGTCTGGCTC TCTGCTGCAG CTGACCTGTC GAGACCATTC AGACCCGCGT 
CAGACCTTTC TCTATAAGCT GAGCAAGAAA GCAGGCCTTC AGTTCTTCAA GAATGTGGTG 
TTGGTGGGCT CTCTTCAGGA CCGATACGTT CCTTATCACT CCGCTCGAAT CGAGATGTGC 
AAAACAGCCT TAAAGGACAA ACAGACTGGT CCGGTGTATG CTGAGATGAT CGAGAACCTT 
CTCCTTCCTG TGCTTCAGAA CAAAGACTGT AACCTGGTGC GTTACGACGT CATCCACGCG 
CTGCCCAACA CCGCCAACTC TCTGATCGGC CGCGCAGCTC ACATCGCCGT CCTGGACTCT 
GAGATTTTCC TGGAAAAGTT CTTCCTGGTG GCAGGGCTCA AGTTTTTTCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam135a ( XM_005157008.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157065.1 Danio rerio family with sequence similarity 135 member A (fam135a), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa18995
Clone ID Related Accession (Same CDS sequence)	XM_005157008.3 , XM_005157008.4
Accession Version	XM_005157008.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein FAM135A isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157008.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGTA 
GAAGAGTCTT TAAATGAAGT TGACTTTCAG CTGTGTTTGG ACCTGTTCTT CACTGATGGA 
GACTACTCGC CTGAGGAGCC CAGTTCTCTG CAGAGCATCA GCAGCCGTAC GCTGCGTTTG 
CACTTTAGCC TCCACAGAGG CATCCACCAC CACGTCAACG TCATGTTTGA CTACTTCCAC 
CTGTCTGTCA TCTCTGCCAC AATCCATGCT TCACTGGTGG CTCTGCATCA GCCTCTAATA 
AGTTTCCCCC GGCCAGTAAA AAGCACCTGG TTGAATCGTA ATGCTCCGGC ACAGAGCAAA 
GACTCCCTCA TCCCACCGCT GGAGACTGTG GTGTTCGGCA GCACCTACGT CAAGCAGCTG 
TCCCCTGATG GCAAAAGCTT CCTGGTATCG GATCAGTGCC TCCAGCATGC CTTCAGCCTG 
CACCAGAACA TGTGTGCCAA CCTGCTGCGG GCATATCAGA GCCTGTTCTG TTACTTAACC 
TCCCTGACCA AGAATCTGCC CGCATCCCAG CGCATGGAGC TTGAGGAACT TGATGTGGAG 
GTCCGACTTC ATGAGCTTTG TGAACAAGTC AAGAAAGCAG AGAGCCCAGA TGAGTTGGCT 
GAATTGGTGA ACATGAATTT GGCGCAACTA TGTTCCCTAC TCATGGCTTT GTGGGGGAAT 
TTCCTGGAAG TTGTGTCACT AAATGAGCGT GTTTTTGTGC TTTTGGCCCA GGAGCACCAC 
ACACTACGGG TGCAAAGATT TGCTGAAGCT TTCTTTTGTC TTGAACACCC ACGACAGAGT 
GCACTGGCCT ACCAGGAACT GCATGCTCAC AGTCACCAAC AAATGACCAC AGCTATCAAA 
AGCTCTTCCT ACTTCTTGTC CTTACCACCT CTTCCTGTTG AATGTGCTGA TCTTGACGGG 
GATGTGAACT CCCTACCAAT CATCTTTGAG GACAGATATT TGGAGTCTGT TACTGAAGAT 
CGTGATGGTC CATGGCTTGG ACTCCAAAAC AACAGGAGTG GCTCTGTGTC CGCCAAAGCA 
GATAGATCCA ACTCAAAGGA CTGCATTGCC AGTGGAGCCC TCGGTCCGTA TCCAGATAAG 
CAGCAAGCTT CCTTTGCCAC TGCTTGGCCT GAACAAAACG AGCCTTCCCT TAAATCCAAA 
GCCAAGTCCA TCAAGCTCAA GAAGAACACC AAGACCGAAA ACTCAAAGAA GCTGATCAGA 
CAGAGCTCGA AAGATTCTGT GGTGCTCACA GGCTACAAGA ACTTGAAGGT TCCTCACAGT 
GAAAAGACCA TTAAGTCCAA AGACATCGAC CATCAATGCA ATCAGAGAGA TGAGCTAGAG 
GATGGAGGTA AATTCCTTCT TCAGAATGAA ACCACGACCA CGCATTGTGC TCAGAATGCT 
AAAGACTGCA CATCTGGCAA CCTTCAAGGT AACGCCACAA TCCTAAGTGA CGGCTTTCCC 
AATCTTATGA GTTTGCAAAT GCAGGCCAGG CCCCAGGCAG GTACTGGAGC AGAGGCCGCC 
GCTGCCAGAG TAGATCCCAC ACATCCACCT GCTGGCCTCA GACATATTGA TGTCAAACCC 
AGTGACAAGG ACCCCTACCA GGGCGATAAG GTCACCGTTC TCTTACCGCA CCACCCAGAG 
CGCACCACCA AGAGGGATGT TCCAGAAATC ACACAGACTG ATTTCTCTGA GTTCAAGAGT 
CGAGTTGGAG ATGGTGCTAC TTTCGTCGGA CCTTGTGGAG CGGCTTTGAG GAACGATAAT 
ATTCCTCAAG CCTCCTCCAG ACTCTCGGAT TCCGGAATTG AAAGCGAACC CAGCTCTGCG 
ACACAGCTAG CCCCGGTTTC TCCATCTCCT TTTGCTCCTA TTGGGATCGA AGCACCTACA 
GAGCTGATTC CTCACAGACC ACCTCCGCTC AGTCCTGCTT CCAGAGGAGC CACCGCATTG 
GAAGGCCTCA ATCCTGAAAG CACCAGCGGC GTAAGCGGCG TCCAATCTTC CCTCACCTCC 
ATCAACTCAC TCCCTTCGGA TGATGAGGTG GAGACAGAGG CCACCAGCAG GAAGTCCAGC 
ATCCTGGTCC AGGAACAGAG TCTCATCTTC TCCGGAGAAT CTCAAAGCCT CTTGCTGACA 
AACTCTAGAG TCTTGGCTCA ACAAAAAGCC AGTCATGGAG ATGGGATGGA CTCTGCTTTG 
CAGTCCCGCT GTGAAACTTC TGCTTCTGAC AGACTTGTCG GATCTAACGA GACAGATCTT 
CCAGGTGGTC CGGAAGCTTG TAGTGAACCA CAGACAGCAG CCCAGTCCAG TACATCAGTC 
ACCAGCAGCA CAGACATGGT GAAGAAAGGC TTTGTAGAAA ACTACTTTGG CTCTCGGTCC 
AGTACTGATA TATCTGAAAT CAGCCCTTTA GAGACGGCAG CCATAACATT GGGTATTCAG 
ACATGCCCCA ATGCTAATGA TGAAGAGGAT GAGGAGGAAG AGCAGGAGCA TGAGATGATT 
GAGAATGGTT ATTATGAGGA GACCGATGGT GTGGTTTATG TTAATGGGCT CCCGGAGGAT 
GAAGGGAGGT GTGAAGATGG AGGAGCGGAG GGAAGAACAC TGTTTTATGA TCATCTTGGT 
TTAGAGCAGC TCCAGGAAAC TTACATTCCC TCTGCCGGCA TTCGGACACG GCCTACTGGA 
CCAGCACCAA CCAGCAGCGA CTTATGCTGG TACAAGAGCG CCCCCACTGC TCAGATGACG 
GCTTTTGTCC AAGCGAAAGA GGAGTTGAAA CAACTAAAGC TGCCAGGCTT CCTGTACAGT 
GAGGTGGCAG ATCTGGCATC CACTGTGCCA TATTTCAGCA TGGATGAGGA TGAAAACTGT 
GATGATGGCA TACACCTTAT AGTGTGTGTA CACGGCTTGG ATGGTAACAG TGCAGATTTG 
CGCCTGGTGA AGACTTATCT GGAGCTTGGG TTGCCTGGAG CTAGGATAGA TTTTCTGATG 
TCTGAGCGAA ATCAGAATGA CACATTTGCT GATTTTGAGT CCATGACCGA CAGACTTTTG 
GATGAAATTG TGCAGTACAT ACAAATCTAC AACCTCACAG TTTCCAAGAT CAGTTTTGTT 
GGTCATTCTC TGGGAAATCT GATCGTGCGC TCTGTGCTGA CGAGGCCCAG GTTTAAGTGT 
TATCTCAGCC GACTGCACAC CTTTCTGTCT CTGTCTGGAC CTCACCTCGG CACACTGTAC 
AACAGCTCAG CACTCGTCAA TACTGGACTG TGGTTCATGC AGAAGTGGAA GAAGTCTGGC 
TCTCTGCTGC AGCTGACCTG TCGAGACCAT TCAGACCCGC GTCAGACCTT TCTCTATAAG 
CTGAGCAAGA AAGCAGGCCT TCAGTTCTTC AAGAATGTGG TGTTGGTGGG CTCTCTTCAG 
GACCGATACG TTCCTTATCA CTCCGCTCGA ATCGAGATGT GCAAAACAGC CTTAAAGGAC 
AAACAGACTG GTCCGGTGTA TGCTGAGATG ATCGAGAACC TTCTCCTTCC TGTGCTTCAG 
AACAAAGACT GTAACCTGGT GCGTTACGAC GTCATCCACG CGCTGCCCAA CACCGCCAAC 
TCTCTGATCG GCCGCGCAGC TCACATCGCC GTCCTGGACT CTGAGATTTT CCTGGAAAAG 
TTCTTCCTGG TGGCAGGGCT CAAGTTTTTT CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157065.1
CDS	388..4323
Translation	MTEVQATVEFSVELHKFYNVDLFQRGFYQVRASMKVPPRIPQRVEASLLHPIGSDLAFPASVQDDVVCSKTFQILYKNEEIVVNDVFVFKVMMLLDAKKVEESLNEVDFQLCLDLFFTDGDYSPEEPSSLQSISSRTLRLHFSLHRGIHHHVNVMFDYFHLSVISATIHASLVALHQPLISFPRPVKSTWLNRNAPAQSKDSLIPPLETVVFGSTYVKQLSPDGKSFLVSDQCLQHAFSLHQNMCANLLRAYQSLFCYLTSLTKNLPASQRMELEELDVEVRLHELCEQVKKAESPDELAELVNMNLAQLCSLLMALWGNFLEVVSLNERVFVLLAQEHHTLRVQRFAEAFFCLEHPRQSALAYQELHAHSHQQMTTAIKSSSYFLSLPPLPVECADLDGDVNSLPIIFEDRYLESVTEDRDGPWLGLQNNRSGSVSAKADRSNSKDCIASGALGPYPDKQQASFATAWPEQNEPSLKSKAKSIKLKKNTKTENSKKLIRQSSKDSVVLTGYKNLKVPHSEKTIKSKDIDHQCNQRDELEDGGKFLLQNETTTTHCAQNAKDCTSGNLQGNATILSDGFPNLMSLQMQARPQAGTGAEAAAARVDPTHPPAGLRHIDVKPSDKDPYQGDKVTVLLPHHPERTTKRDVPEITQTDFSEFKSRVGDGATFVGPCGAALRNDNIPQASSRLSDSGIESEPSSATQLAPVSPSPFAPIGIEAPTELIPHRPPPLSPASRGATALEGLNPESTSGVSGVQSSLTSINSLPSDDEVETEATSRKSSILVQEQSLIFSGESQSLLLTNSRVLAQQKASHGDGMDSALQSRCETSASDRLVGSNETDLPGGPEACSEPQTAAQSSTSVTSSTDMVKKGFVENYFGSRSSTDISEISPLETAAITLGIQTCPNANDEEDEEEEQEHEMIENGYYEETDGVVYVNGLPEDEGRCEDGGAEGRTLFYDHLGLEQLQETYIPSAGIRTRPTGPAPTSSDLCWYKSAPTAQMTAFVQAKEELKQLKLPGFLYSEVADLASTVPYFSMDEDENCDDGIHLIVCVHGLDGNSADLRLVKTYLELGLPGARIDFLMSERNQNDTFADFESMTDRLLDEIVQYIQIYNLTVSKISFVGHSLGNLIVRSVLTRPRFKCYLSRLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSKKAGLQFFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQTGPVYAEMIENLLLPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEIFLEKFFLVAGLKFFQ

Target ORF information:

RefSeq Version	XM_005157008.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio family with sequence similarity 135 member A (fam135a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157008.3

ORF Insert Sequence:

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGTA 
GAAGAGTCTT TAAATGAAGT TGACTTTCAG CTGTGTTTGG ACCTGTTCTT CACTGATGGA 
GACTACTCGC CTGAGGAGCC CAGTTCTCTG CAGAGCATCA GCAGCCGTAC GCTGCGTTTG 
CACTTTAGCC TCCACAGAGG CATCCACCAC CACGTCAACG TCATGTTTGA CTACTTCCAC 
CTGTCTGTCA TCTCTGCCAC AATCCATGCT TCACTGGTGG CTCTGCATCA GCCTCTAATA 
AGTTTCCCCC GGCCAGTAAA AAGCACCTGG TTGAATCGTA ATGCTCCGGC ACAGAGCAAA 
GACTCCCTCA TCCCACCGCT GGAGACTGTG GTGTTCGGCA GCACCTACGT CAAGCAGCTG 
TCCCCTGATG GCAAAAGCTT CCTGGTATCG GATCAGTGCC TCCAGCATGC CTTCAGCCTG 
CACCAGAACA TGTGTGCCAA CCTGCTGCGG GCATATCAGA GCCTGTTCTG TTACTTAACC 
TCCCTGACCA AGAATCTGCC CGCATCCCAG CGCATGGAGC TTGAGGAACT TGATGTGGAG 
GTCCGACTTC ATGAGCTTTG TGAACAAGTC AAGAAAGCAG AGAGCCCAGA TGAGTTGGCT 
GAATTGGTGA ACATGAATTT GGCGCAACTA TGTTCCCTAC TCATGGCTTT GTGGGGGAAT 
TTCCTGGAAG TTGTGTCACT AAATGAGCGT GTTTTTGTGC TTTTGGCCCA GGAGCACCAC 
ACACTACGGG TGCAAAGATT TGCTGAAGCT TTCTTTTGTC TTGAACACCC ACGACAGAGT 
GCACTGGCCT ACCAGGAACT GCATGCTCAC AGTCACCAAC AAATGACCAC AGCTATCAAA 
AGCTCTTCCT ACTTCTTGTC CTTACCACCT CTTCCTGTTG AATGTGCTGA TCTTGACGGG 
GATGTGAACT CCCTACCAAT CATCTTTGAG GACAGATATT TGGAGTCTGT TACTGAAGAT 
CGTGATGGTC CATGGCTTGG ACTCCAAAAC AACAGGAGTG GCTCTGTGTC CGCCAAAGCA 
GATAGATCCA ACTCAAAGGA CTGCATTGCC AGTGGAGCCC TCGGTCCGTA TCCAGATAAG 
CAGCAAGCTT CCTTTGCCAC TGCTTGGCCT GAACAAAACG AGCCTTCCCT TAAATCCAAA 
GCCAAGTCCA TCAAGCTCAA GAAGAACACC AAGACCGAAA ACTCAAAGAA GCTGATCAGA 
CAGAGCTCGA AAGATTCTGT GGTGCTCACA GGCTACAAGA ACTTGAAGGT TCCTCACAGT 
GAAAAGACCA TTAAGTCCAA AGACATCGAC CATCAATGCA ATCAGAGAGA TGAGCTAGAG 
GATGGAGGTA AATTCCTTCT TCAGAATGAA ACCACGACCA CGCATTGTGC TCAGAATGCT 
AAAGACTGCA CATCTGGCAA CCTTCAAGGT AACGCCACAA TCCTAAGTGA CGGCTTTCCC 
AATCTTATGA GTTTGCAAAT GCAGGCCAGG CCCCAGGCAG GTACTGGAGC AGAGGCCGCC 
GCTGCCAGAG TAGATCCCAC ACATCCACCT GCTGGCCTCA GACATATTGA TGTCAAACCC 
AGTGACAAGG ACCCCTACCA GGGCGATAAG GTCACCGTTC TCTTACCGCA CCACCCAGAG 
CGCACCACCA AGAGGGATGT TCCAGAAATC ACACAGACTG ATTTCTCTGA GTTCAAGAGT 
CGAGTTGGAG ATGGTGCTAC TTTCGTCGGA CCTTGTGGAG CGGCTTTGAG GAACGATAAT 
ATTCCTCAAG CCTCCTCCAG ACTCTCGGAT TCCGGAATTG AAAGCGAACC CAGCTCTGCG 
ACACAGCTAG CCCCGGTTTC TCCATCTCCT TTTGCTCCTA TTGGGATCGA AGCACCTACA 
GAGCTGATTC CTCACAGACC ACCTCCGCTC AGTCCTGCTT CCAGAGGAGC CACCGCATTG 
GAAGGCCTCA ATCCTGAAAG CACCAGCGGC GTAAGCGGCG TCCAATCTTC CCTCACCTCC 
ATCAACTCAC TCCCTTCGGA TGATGAGGTG GAGACAGAGG CCACCAGCAG GAAGTCCAGC 
ATCCTGGTCC AGGAACAGAG TCTCATCTTC TCCGGAGAAT CTCAAAGCCT CTTGCTGACA 
AACTCTAGAG TCTTGGCTCA ACAAAAAGCC AGTCATGGAG ATGGGATGGA CTCTGCTTTG 
CAGTCCCGCT GTGAAACTTC TGCTTCTGAC AGACTTGTCG GATCTAACGA GACAGATCTT 
CCAGGTGGTC CGGAAGCTTG TAGTGAACCA CAGACAGCAG CCCAGTCCAG TACATCAGTC 
ACCAGCAGCA CAGACATGGT GAAGAAAGGC TTTGTAGAAA ACTACTTTGG CTCTCGGTCC 
AGTACTGATA TATCTGAAAT CAGCCCTTTA GAGACGGCAG CCATAACATT GGGTATTCAG 
ACATGCCCCA ATGCTAATGA TGAAGAGGAT GAGGAGGAAG AGCAGGAGCA TGAGATGATT 
GAGAATGGTT ATTATGAGGA GACCGATGGT GTGGTTTATG TTAATGGGCT CCCGGAGGAT 
GAAGGGAGGT GTGAAGATGG AGGAGCGGAG GGAAGAACAC TGTTTTATGA TCATCTTGGT 
TTAGAGCAGC TCCAGGAAAC TTACATTCCC TCTGCCGGCA TTCGGACACG GCCTACTGGA 
CCAGCACCAA CCAGCAGCGA CTTATGCTGG TACAAGAGCG CCCCCACTGC TCAGATGACG 
GCTTTTGTCC AAGCGAAAGA GGAGTTGAAA CAACTAAAGC TGCCAGGCTT CCTGTACAGT 
GAGGTGGCAG ATCTGGCATC CACTGTGCCA TATTTCAGCA TGGATGAGGA TGAAAACTGT 
GATGATGGCA TACACCTTAT AGTGTGTGTA CACGGCTTGG ATGGTAACAG TGCAGATTTG 
CGCCTGGTGA AGACTTATCT GGAGCTTGGG TTGCCTGGAG CTAGGATAGA TTTTCTGATG 
TCTGAGCGAA ATCAGAATGA CACATTTGCT GATTTTGAGT CCATGACCGA CAGACTTTTG 
GATGAAATTG TGCAGTACAT ACAAATCTAC AACCTCACAG TTTCCAAGAT CAGTTTTGTT 
GGTCATTCTC TGGGAAATCT GATCGTGCGC TCTGTGCTGA CGAGGCCCAG GTTTAAGTGT 
TATCTCAGCC GACTGCACAC CTTTCTGTCT CTGTCTGGAC CTCACCTCGG CACACTGTAC 
AACAGCTCAG CACTCGTCAA TACTGGACTG TGGTTCATGC AGAAGTGGAA GAAGTCTGGC 
TCTCTGCTGC AGCTGACCTG TCGAGACCAT TCAGACCCGC GTCAGACCTT TCTCTATAAG 
CTGAGCAAGA AAGCAGGCCT TCAGTTCTTC AAGAATGTGG TGTTGGTGGG CTCTCTTCAG 
GACCGATACG TTCCTTATCA CTCCGCTCGA ATCGAGATGT GCAAAACAGC CTTAAAGGAC 
AAACAGACTG GTCCGGTGTA TGCTGAGATG ATCGAGAACC TTCTCCTTCC TGTGCTTCAG 
AACAAAGACT GTAACCTGGT GCGTTACGAC GTCATCCACG CGCTGCCCAA CACCGCCAAC 
TCTCTGATCG GCCGCGCAGC TCACATCGCC GTCCTGGACT CTGAGATTTT CCTGGAAAAG 
TTCTTCCTGG TGGCAGGGCT CAAGTTTTTT CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam135a ( XM_005157008.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157065.1 Danio rerio family with sequence similarity 135, member A (fam135a), transcript variant X7, mRNA.

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CloneID	ODa18995
Clone ID Related Accession (Same CDS sequence)	XM_005157008.3 , XM_005157008.4
Accession Version	XM_005157008.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein FAM135A isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157008.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGTA 
GAAGAGTCTT TAAATGAAGT TGACTTTCAG CTGTGTTTGG ACCTGTTCTT CACTGATGGA 
GACTACTCGC CTGAGGAGCC CAGTTCTCTG CAGAGCATCA GCAGCCGTAC GCTGCGTTTG 
CACTTTAGCC TCCACAGAGG CATCCACCAC CACGTCAACG TCATGTTTGA CTACTTCCAC 
CTGTCTGTCA TCTCTGCCAC AATCCATGCT TCACTGGTGG CTCTGCATCA GCCTCTAATA 
AGTTTCCCCC GGCCAGTAAA AAGCACCTGG TTGAATCGTA ATGCTCCGGC ACAGAGCAAA 
GACTCCCTCA TCCCACCGCT GGAGACTGTG GTGTTCGGCA GCACCTACGT CAAGCAGCTG 
TCCCCTGATG GCAAAAGCTT CCTGGTATCG GATCAGTGCC TCCAGCATGC CTTCAGCCTG 
CACCAGAACA TGTGTGCCAA CCTGCTGCGG GCATATCAGA GCCTGTTCTG TTACTTAACC 
TCCCTGACCA AGAATCTGCC CGCATCCCAG CGCATGGAGC TTGAGGAACT TGATGTGGAG 
GTCCGACTTC ATGAGCTTTG TGAACAAGTC AAGAAAGCAG AGAGCCCAGA TGAGTTGGCT 
GAATTGGTGA ACATGAATTT GGCGCAACTA TGTTCCCTAC TCATGGCTTT GTGGGGGAAT 
TTCCTGGAAG TTGTGTCACT AAATGAGCGT GTTTTTGTGC TTTTGGCCCA GGAGCACCAC 
ACACTACGGG TGCAAAGATT TGCTGAAGCT TTCTTTTGTC TTGAACACCC ACGACAGAGT 
GCACTGGCCT ACCAGGAACT GCATGCTCAC AGTCACCAAC AAATGACCAC AGCTATCAAA 
AGCTCTTCCT ACTTCTTGTC CTTACCACCT CTTCCTGTTG AATGTGCTGA TCTTGACGGG 
GATGTGAACT CCCTACCAAT CATCTTTGAG GACAGATATT TGGAGTCTGT TACTGAAGAT 
CGTGATGGTC CATGGCTTGG ACTCCAAAAC AACAGGAGTG GCTCTGTGTC CGCCAAAGCA 
GATAGATCCA ACTCAAAGGA CTGCATTGCC AGTGGAGCCC TCGGTCCGTA TCCAGATAAG 
CAGCAAGCTT CCTTTGCCAC TGCTTGGCCT GAACAAAACG AGCCTTCCCT TAAATCCAAA 
GCCAAGTCCA TCAAGCTCAA GAAGAACACC AAGACCGAAA ACTCAAAGAA GCTGATCAGA 
CAGAGCTCGA AAGATTCTGT GGTGCTCACA GGCTACAAGA ACTTGAAGGT TCCTCACAGT 
GAAAAGACCA TTAAGTCCAA AGACATCGAC CATCAATGCA ATCAGAGAGA TGAGCTAGAG 
GATGGAGGTA AATTCCTTCT TCAGAATGAA ACCACGACCA CGCATTGTGC TCAGAATGCT 
AAAGACTGCA CATCTGGCAA CCTTCAAGGT AACGCCACAA TCCTAAGTGA CGGCTTTCCC 
AATCTTATGA GTTTGCAAAT GCAGGCCAGG CCCCAGGCAG GTACTGGAGC AGAGGCCGCC 
GCTGCCAGAG TAGATCCCAC ACATCCACCT GCTGGCCTCA GACATATTGA TGTCAAACCC 
AGTGACAAGG ACCCCTACCA GGGCGATAAG GTCACCGTTC TCTTACCGCA CCACCCAGAG 
CGCACCACCA AGAGGGATGT TCCAGAAATC ACACAGACTG ATTTCTCTGA GTTCAAGAGT 
CGAGTTGGAG ATGGTGCTAC TTTCGTCGGA CCTTGTGGAG CGGCTTTGAG GAACGATAAT 
ATTCCTCAAG CCTCCTCCAG ACTCTCGGAT TCCGGAATTG AAAGCGAACC CAGCTCTGCG 
ACACAGCTAG CCCCGGTTTC TCCATCTCCT TTTGCTCCTA TTGGGATCGA AGCACCTACA 
GAGCTGATTC CTCACAGACC ACCTCCGCTC AGTCCTGCTT CCAGAGGAGC CACCGCATTG 
GAAGGCCTCA ATCCTGAAAG CACCAGCGGC GTAAGCGGCG TCCAATCTTC CCTCACCTCC 
ATCAACTCAC TCCCTTCGGA TGATGAGGTG GAGACAGAGG CCACCAGCAG GAAGTCCAGC 
ATCCTGGTCC AGGAACAGAG TCTCATCTTC TCCGGAGAAT CTCAAAGCCT CTTGCTGACA 
AACTCTAGAG TCTTGGCTCA ACAAAAAGCC AGTCATGGAG ATGGGATGGA CTCTGCTTTG 
CAGTCCCGCT GTGAAACTTC TGCTTCTGAC AGACTTGTCG GATCTAACGA GACAGATCTT 
CCAGGTGGTC CGGAAGCTTG TAGTGAACCA CAGACAGCAG CCCAGTCCAG TACATCAGTC 
ACCAGCAGCA CAGACATGGT GAAGAAAGGC TTTGTAGAAA ACTACTTTGG CTCTCGGTCC 
AGTACTGATA TATCTGAAAT CAGCCCTTTA GAGACGGCAG CCATAACATT GGGTATTCAG 
ACATGCCCCA ATGCTAATGA TGAAGAGGAT GAGGAGGAAG AGCAGGAGCA TGAGATGATT 
GAGAATGGTT ATTATGAGGA GACCGATGGT GTGGTTTATG TTAATGGGCT CCCGGAGGAT 
GAAGGGAGGT GTGAAGATGG AGGAGCGGAG GGAAGAACAC TGTTTTATGA TCATCTTGGT 
TTAGAGCAGC TCCAGGAAAC TTACATTCCC TCTGCCGGCA TTCGGACACG GCCTACTGGA 
CCAGCACCAA CCAGCAGCGA CTTATGCTGG TACAAGAGCG CCCCCACTGC TCAGATGACG 
GCTTTTGTCC AAGCGAAAGA GGAGTTGAAA CAACTAAAGC TGCCAGGCTT CCTGTACAGT 
GAGGTGGCAG ATCTGGCATC CACTGTGCCA TATTTCAGCA TGGATGAGGA TGAAAACTGT 
GATGATGGCA TACACCTTAT AGTGTGTGTA CACGGCTTGG ATGGTAACAG TGCAGATTTG 
CGCCTGGTGA AGACTTATCT GGAGCTTGGG TTGCCTGGAG CTAGGATAGA TTTTCTGATG 
TCTGAGCGAA ATCAGAATGA CACATTTGCT GATTTTGAGT CCATGACCGA CAGACTTTTG 
GATGAAATTG TGCAGTACAT ACAAATCTAC AACCTCACAG TTTCCAAGAT CAGTTTTGTT 
GGTCATTCTC TGGGAAATCT GATCGTGCGC TCTGTGCTGA CGAGGCCCAG GTTTAAGTGT 
TATCTCAGCC GACTGCACAC CTTTCTGTCT CTGTCTGGAC CTCACCTCGG CACACTGTAC 
AACAGCTCAG CACTCGTCAA TACTGGACTG TGGTTCATGC AGAAGTGGAA GAAGTCTGGC 
TCTCTGCTGC AGCTGACCTG TCGAGACCAT TCAGACCCGC GTCAGACCTT TCTCTATAAG 
CTGAGCAAGA AAGCAGGCCT TCAGTTCTTC AAGAATGTGG TGTTGGTGGG CTCTCTTCAG 
GACCGATACG TTCCTTATCA CTCCGCTCGA ATCGAGATGT GCAAAACAGC CTTAAAGGAC 
AAACAGACTG GTCCGGTGTA TGCTGAGATG ATCGAGAACC TTCTCCTTCC TGTGCTTCAG 
AACAAAGACT GTAACCTGGT GCGTTACGAC GTCATCCACG CGCTGCCCAA CACCGCCAAC 
TCTCTGATCG GCCGCGCAGC TCACATCGCC GTCCTGGACT CTGAGATTTT CCTGGAAAAG 
TTCTTCCTGG TGGCAGGGCT CAAGTTTTTT CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157065.1
CDS	405..4340
Translation	MTEVQATVEFSVELHKFYNVDLFQRGFYQVRASMKVPPRIPQRVEASLLHPIGSDLAFPASVQDDVVCSKTFQILYKNEEIVVNDVFVFKVMMLLDAKKVEESLNEVDFQLCLDLFFTDGDYSPEEPSSLQSISSRTLRLHFSLHRGIHHHVNVMFDYFHLSVISATIHASLVALHQPLISFPRPVKSTWLNRNAPAQSKDSLIPPLETVVFGSTYVKQLSPDGKSFLVSDQCLQHAFSLHQNMCANLLRAYQSLFCYLTSLTKNLPASQRMELEELDVEVRLHELCEQVKKAESPDELAELVNMNLAQLCSLLMALWGNFLEVVSLNERVFVLLAQEHHTLRVQRFAEAFFCLEHPRQSALAYQELHAHSHQQMTTAIKSSSYFLSLPPLPVECADLDGDVNSLPIIFEDRYLESVTEDRDGPWLGLQNNRSGSVSAKADRSNSKDCIASGALGPYPDKQQASFATAWPEQNEPSLKSKAKSIKLKKNTKTENSKKLIRQSSKDSVVLTGYKNLKVPHSEKTIKSKDIDHQCNQRDELEDGGKFLLQNETTTTHCAQNAKDCTSGNLQGNATILSDGFPNLMSLQMQARPQAGTGAEAAAARVDPTHPPAGLRHIDVKPSDKDPYQGDKVTVLLPHHPERTTKRDVPEITQTDFSEFKSRVGDGATFVGPCGAALRNDNIPQASSRLSDSGIESEPSSATQLAPVSPSPFAPIGIEAPTELIPHRPPPLSPASRGATALEGLNPESTSGVSGVQSSLTSINSLPSDDEVETEATSRKSSILVQEQSLIFSGESQSLLLTNSRVLAQQKASHGDGMDSALQSRCETSASDRLVGSNETDLPGGPEACSEPQTAAQSSTSVTSSTDMVKKGFVENYFGSRSSTDISEISPLETAAITLGIQTCPNANDEEDEEEEQEHEMIENGYYEETDGVVYVNGLPEDEGRCEDGGAEGRTLFYDHLGLEQLQETYIPSAGIRTRPTGPAPTSSDLCWYKSAPTAQMTAFVQAKEELKQLKLPGFLYSEVADLASTVPYFSMDEDENCDDGIHLIVCVHGLDGNSADLRLVKTYLELGLPGARIDFLMSERNQNDTFADFESMTDRLLDEIVQYIQIYNLTVSKISFVGHSLGNLIVRSVLTRPRFKCYLSRLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSKKAGLQFFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQTGPVYAEMIENLLLPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEIFLEKFFLVAGLKFFQ

Target ORF information:

RefSeq Version	XM_005157008.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio family with sequence similarity 135, member A (fam135a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157008.4

ORF Insert Sequence:

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGTA 
GAAGAGTCTT TAAATGAAGT TGACTTTCAG CTGTGTTTGG ACCTGTTCTT CACTGATGGA 
GACTACTCGC CTGAGGAGCC CAGTTCTCTG CAGAGCATCA GCAGCCGTAC GCTGCGTTTG 
CACTTTAGCC TCCACAGAGG CATCCACCAC CACGTCAACG TCATGTTTGA CTACTTCCAC 
CTGTCTGTCA TCTCTGCCAC AATCCATGCT TCACTGGTGG CTCTGCATCA GCCTCTAATA 
AGTTTCCCCC GGCCAGTAAA AAGCACCTGG TTGAATCGTA ATGCTCCGGC ACAGAGCAAA 
GACTCCCTCA TCCCACCGCT GGAGACTGTG GTGTTCGGCA GCACCTACGT CAAGCAGCTG 
TCCCCTGATG GCAAAAGCTT CCTGGTATCG GATCAGTGCC TCCAGCATGC CTTCAGCCTG 
CACCAGAACA TGTGTGCCAA CCTGCTGCGG GCATATCAGA GCCTGTTCTG TTACTTAACC 
TCCCTGACCA AGAATCTGCC CGCATCCCAG CGCATGGAGC TTGAGGAACT TGATGTGGAG 
GTCCGACTTC ATGAGCTTTG TGAACAAGTC AAGAAAGCAG AGAGCCCAGA TGAGTTGGCT 
GAATTGGTGA ACATGAATTT GGCGCAACTA TGTTCCCTAC TCATGGCTTT GTGGGGGAAT 
TTCCTGGAAG TTGTGTCACT AAATGAGCGT GTTTTTGTGC TTTTGGCCCA GGAGCACCAC 
ACACTACGGG TGCAAAGATT TGCTGAAGCT TTCTTTTGTC TTGAACACCC ACGACAGAGT 
GCACTGGCCT ACCAGGAACT GCATGCTCAC AGTCACCAAC AAATGACCAC AGCTATCAAA 
AGCTCTTCCT ACTTCTTGTC CTTACCACCT CTTCCTGTTG AATGTGCTGA TCTTGACGGG 
GATGTGAACT CCCTACCAAT CATCTTTGAG GACAGATATT TGGAGTCTGT TACTGAAGAT 
CGTGATGGTC CATGGCTTGG ACTCCAAAAC AACAGGAGTG GCTCTGTGTC CGCCAAAGCA 
GATAGATCCA ACTCAAAGGA CTGCATTGCC AGTGGAGCCC TCGGTCCGTA TCCAGATAAG 
CAGCAAGCTT CCTTTGCCAC TGCTTGGCCT GAACAAAACG AGCCTTCCCT TAAATCCAAA 
GCCAAGTCCA TCAAGCTCAA GAAGAACACC AAGACCGAAA ACTCAAAGAA GCTGATCAGA 
CAGAGCTCGA AAGATTCTGT GGTGCTCACA GGCTACAAGA ACTTGAAGGT TCCTCACAGT 
GAAAAGACCA TTAAGTCCAA AGACATCGAC CATCAATGCA ATCAGAGAGA TGAGCTAGAG 
GATGGAGGTA AATTCCTTCT TCAGAATGAA ACCACGACCA CGCATTGTGC TCAGAATGCT 
AAAGACTGCA CATCTGGCAA CCTTCAAGGT AACGCCACAA TCCTAAGTGA CGGCTTTCCC 
AATCTTATGA GTTTGCAAAT GCAGGCCAGG CCCCAGGCAG GTACTGGAGC AGAGGCCGCC 
GCTGCCAGAG TAGATCCCAC ACATCCACCT GCTGGCCTCA GACATATTGA TGTCAAACCC 
AGTGACAAGG ACCCCTACCA GGGCGATAAG GTCACCGTTC TCTTACCGCA CCACCCAGAG 
CGCACCACCA AGAGGGATGT TCCAGAAATC ACACAGACTG ATTTCTCTGA GTTCAAGAGT 
CGAGTTGGAG ATGGTGCTAC TTTCGTCGGA CCTTGTGGAG CGGCTTTGAG GAACGATAAT 
ATTCCTCAAG CCTCCTCCAG ACTCTCGGAT TCCGGAATTG AAAGCGAACC CAGCTCTGCG 
ACACAGCTAG CCCCGGTTTC TCCATCTCCT TTTGCTCCTA TTGGGATCGA AGCACCTACA 
GAGCTGATTC CTCACAGACC ACCTCCGCTC AGTCCTGCTT CCAGAGGAGC CACCGCATTG 
GAAGGCCTCA ATCCTGAAAG CACCAGCGGC GTAAGCGGCG TCCAATCTTC CCTCACCTCC 
ATCAACTCAC TCCCTTCGGA TGATGAGGTG GAGACAGAGG CCACCAGCAG GAAGTCCAGC 
ATCCTGGTCC AGGAACAGAG TCTCATCTTC TCCGGAGAAT CTCAAAGCCT CTTGCTGACA 
AACTCTAGAG TCTTGGCTCA ACAAAAAGCC AGTCATGGAG ATGGGATGGA CTCTGCTTTG 
CAGTCCCGCT GTGAAACTTC TGCTTCTGAC AGACTTGTCG GATCTAACGA GACAGATCTT 
CCAGGTGGTC CGGAAGCTTG TAGTGAACCA CAGACAGCAG CCCAGTCCAG TACATCAGTC 
ACCAGCAGCA CAGACATGGT GAAGAAAGGC TTTGTAGAAA ACTACTTTGG CTCTCGGTCC 
AGTACTGATA TATCTGAAAT CAGCCCTTTA GAGACGGCAG CCATAACATT GGGTATTCAG 
ACATGCCCCA ATGCTAATGA TGAAGAGGAT GAGGAGGAAG AGCAGGAGCA TGAGATGATT 
GAGAATGGTT ATTATGAGGA GACCGATGGT GTGGTTTATG TTAATGGGCT CCCGGAGGAT 
GAAGGGAGGT GTGAAGATGG AGGAGCGGAG GGAAGAACAC TGTTTTATGA TCATCTTGGT 
TTAGAGCAGC TCCAGGAAAC TTACATTCCC TCTGCCGGCA TTCGGACACG GCCTACTGGA 
CCAGCACCAA CCAGCAGCGA CTTATGCTGG TACAAGAGCG CCCCCACTGC TCAGATGACG 
GCTTTTGTCC AAGCGAAAGA GGAGTTGAAA CAACTAAAGC TGCCAGGCTT CCTGTACAGT 
GAGGTGGCAG ATCTGGCATC CACTGTGCCA TATTTCAGCA TGGATGAGGA TGAAAACTGT 
GATGATGGCA TACACCTTAT AGTGTGTGTA CACGGCTTGG ATGGTAACAG TGCAGATTTG 
CGCCTGGTGA AGACTTATCT GGAGCTTGGG TTGCCTGGAG CTAGGATAGA TTTTCTGATG 
TCTGAGCGAA ATCAGAATGA CACATTTGCT GATTTTGAGT CCATGACCGA CAGACTTTTG 
GATGAAATTG TGCAGTACAT ACAAATCTAC AACCTCACAG TTTCCAAGAT CAGTTTTGTT 
GGTCATTCTC TGGGAAATCT GATCGTGCGC TCTGTGCTGA CGAGGCCCAG GTTTAAGTGT 
TATCTCAGCC GACTGCACAC CTTTCTGTCT CTGTCTGGAC CTCACCTCGG CACACTGTAC 
AACAGCTCAG CACTCGTCAA TACTGGACTG TGGTTCATGC AGAAGTGGAA GAAGTCTGGC 
TCTCTGCTGC AGCTGACCTG TCGAGACCAT TCAGACCCGC GTCAGACCTT TCTCTATAAG 
CTGAGCAAGA AAGCAGGCCT TCAGTTCTTC AAGAATGTGG TGTTGGTGGG CTCTCTTCAG 
GACCGATACG TTCCTTATCA CTCCGCTCGA ATCGAGATGT GCAAAACAGC CTTAAAGGAC 
AAACAGACTG GTCCGGTGTA TGCTGAGATG ATCGAGAACC TTCTCCTTCC TGTGCTTCAG 
AACAAAGACT GTAACCTGGT GCGTTACGAC GTCATCCACG CGCTGCCCAA CACCGCCAAC 
TCTCTGATCG GCCGCGCAGC TCACATCGCC GTCCTGGACT CTGAGATTTT CCTGGAAAAG 
TTCTTCCTGG TGGCAGGGCT CAAGTTTTTT CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam135a ( NM_001145594.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001139066.1 Danio rerio family with sequence similarity 135 member A (fam135a), mRNA.

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CloneID	ODa18993
Clone ID Related Accession (Same CDS sequence)	XM_005157006.4 , NM_001145594.1 , XM_005157006.3
Accession Version	NM_001145594.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-07
Organism	Danio rerio(zebrafish)
Product	protein FAM135A
Comment	Comment: PREDICTED REFSEQ: This record has not been reviewed and the function is unknown. The reference sequence was derived from CT737228.5 and BX324188.7. On Mar 5, 2009 this sequence version replaced XM_001921847.1. ##Evidence-Data-START## CDS exon combination :: GDQH01004434.1, GFIL01023942.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGTA 
GAAGAGTCTT TAAATGAAGT TGACTTTCAG CTGTGTTTGG ACCTGTTCTT CACTGATGGA 
GACTACTCGC CTGAGGAGCC CAGTTCTCTG CAGAGCATCA GCAGCCGTAC GCTGCGTTTG 
CACTTTAGCC TCCACAGAGG CATCCACCAC CACGTCAACG TCATGTTTGA CTACTTCCAC 
CTGTCTGTCA TCTCTGCCAC AATCCATGCT TCACTGGTGG CTCTGCATCA GCCTCTAATA 
AGTTTCCCCC GGCCAGTAAA AAGCACCTGG TTGAATCGTA ATGCTCCGGC ACAGAGCAAA 
GACTCCCTCA TCCCACCGCT GGAGACTGTG GTGTTCGGCA GCACCTACGT CAAGCAGCTG 
TCCCCTGATG GCAAAAGCTT CCTGGTATCG GATCAGTGCC TCCAGCATGC CTTCAGCCTG 
CACCAGAACA TGTGTGCCAA CCTGCTGCGG GCATATCAGA GCCTGTTCTG TTACTTAACC 
TCCCTGACCA AGAATCTGCC CGCATCCCAG CGCATGGAGC TTGCCAAGCC CAGCATGCAG 
GTCCTTTATG AACGAGTTCT CAGAAGACCT TACCCTCCAG GCCAAAGGCA CGTCTACATA 
GAGGAACTTG ATGTGGAGGT CCGACTTCAT GAGCTTTGTG AACAAGTCAA GCAGAAAGCA 
GAGAGCCCAG ATGAGTTGGC TGAATTGGTG AACATGAATT TGGCGCAACT ATGTTCCCTA 
CTCATGGCTT TGTGGGGGAA TTTCCTGGAA GTTGTGTCAC TAAATGAGCG TGTTTTTGTG 
CTTTTGGCCC AGGAGCACCA CACACTACGG GTGCAAAGAT TTGCTGAAGC TTTCTTTTGT 
CTTGAACACC CACGACAGAG TGCACTGGCC TACCAGGAAC TGCATGCTCA CAGTCACCAA 
CAAATGACCA CAGCTATCAA AAGCTCTTCC TACTTCTTGT CCTTACCACC TCTTCCTGTT 
GAATGTGCTG ATCTTGACGG GGATGTGAAC TCCCTACCAA TCATCTTTGA GGACAGATAT 
TTGGAGTCTG TTACTGAAGA TCGTGATGGT CCATGGCTTG GACTCCAAAA CAACAGGAGT 
GGCTCTGTGT CCGCCAAAGC AGATAGATCC AACTCAAAGG ACTGCATTGC CAGTGGAGCC 
CTCGGTCCGT ATCCAGATAA GCAGCAAGCT TCCTTTGCCA CTGCTTGGCC TGAACAAAAC 
GAGCCTTCCC TTAAATCCAA AGCCAAGTCC ATCAAGCTCA AGAAGAACAC CAAGACCGAA 
AACTCAAAGA AGCTGATCAG ACAGAGCTCG AAAGATTCTG TGGTGCTCAC AGGCTACAAG 
AACTTGAAGG TTCCTCACAG TGAAAAGACC ATTAAGTCCA AAGACATCGA CCATCAATGC 
AATCAGAGAG ATGAGCTAGA GGATGGAGGT AAATTCCTTC TTCAGAATGA AACCACGACC 
ACGCATTGTG CTCAGAATGC TAAAGACTGC ACATCTGGCA ACCTTCAAGG TAACGCCACA 
ATCCTAAGTG ACGGCTTTCC CAATCTTATG AGTTTGCAAA TGCAGGCCAG GCCCCAGGCA 
GGTACTGGAG CAGAGGCCGC CGCTGCCAGA GTAGATCCCA CACATCCACC TGCTGGCCTC 
AGACATATTG ATGTCAAACC CAGTGACAAG GACCCCTACC AGGGCGATAA GGTCACCGTT 
CTCTTACCGC ACCACCCAGA GCGCACCACC AAGAGGGATG TTCCAGAAAT CACACAGACT 
GATTTCTCTG AGTTCAAGAG TCGAGTTGGA GATGGTGCTA CTTTCGTCGG ACCTTGTGGA 
GCGGCTTTGA GGAACGATAA TATTCCTCAA GCCTCCTCCA GACTCTCGGA TTCCGGAATT 
GAAAGCGAAC CCAGCTCTGC GACACAGCTA GCCCCGGTTT CTCCATCTCC TTTTGCTCCT 
ATTGGGATCG AAGCACCTAC AGAGCTGATT CCTCACAGAC CACCTCCGCT CAGTCCTGCT 
TCCAGAGGAG CCACCGCATT GGAAGGCCTC AATCCTGAAA GCACCAGCGG CGTAAGCGGC 
GTCCAATCTT CCCTCACCTC CATCAACTCA CTCCCTTCGG ATGATGAGGT GGAGACAGAG 
GCCACCAGCA GGAAGTCCAG CATCCTGGTC CAGGAACAGA GTCTCATCTT CTCCGGAGAA 
TCTCAAAGCC TCTTGCTGAC AAACTCTAGA GTCTTGGCTC AACAAAAAGC CAGTCATGGA 
GATGGGATGG ACTCTGCTTT GCAGTCCCGC TGTGAAACTT CTGCTTCTGA CAGACTTGTC 
GGATCTAACG AGACAGATCT TCCAGGTGGT CCGGAAGCTT GTAGTGAACC ACAGACAGCA 
GCCCAGTCCA GTACATCAGT CACCAGCAGC ACAGACATGG TGAAGAAAGG CTTTGTAGAA 
AACTACTTTG GCTCTCGGTC CAGTACTGAT ATATCTGAAA TCAGCCCTTT AGAGACGGCA 
GCCATAACAT TGGGTATTCA GACATGCCCC AATGCTAATG ATGAAGAGGA TGAGGAGGAA 
GAGCAGGAGC ATGAGATGAT TGAGAATGGT TATTATGAGG AGACCGATGG TGTGGTTTAT 
GTTAATGGGC TCCCGGAGGA TGAAGGGAGG TGTGAAGATG GAGGAGCGGA GGGAAGAACA 
CTGTTTTATG ATCATCTTGG TTTAGAGCAG CTCCAGGAAA CTTACATTCC CTCTGCCGGC 
ATTCGGACAC GGCCTACTGG ACCAGCACCA ACCAGCAGCG ACTTATGCTG GTACAAGAGC 
GCCCCCACTG CTCAGATGAC GGCTTTTGTC CAAGCGAAAG AGGAGTTGAA ACAACTAAAG 
CTGCCAGGCT TCCTGTACAG TGAGGTGGCA GATCTGGCAT CCACTGTGCC ATATTTCAGC 
ATGGATGAGG ATGAAAACTG TGATGATGGC ATACACCTTA TAGTGTGTGT ACACGGCTTG 
GATGGTAACA GTGCAGATTT GCGCCTGGTG AAGACTTATC TGGAGCTTGG GTTGCCTGGA 
GCTAGGATAG ATTTTCTGAT GTCTGAGCGA AATCAGAATG ACACATTTGC TGATTTTGAG 
TCCATGACCG ACAGACTTTT GGATGAAATT GTGCAGTACA TACAAATCTA CAACCTCACA 
GTTTCCAAGA TCAGTTTTGT TGGTCATTCT CTGGGAAATC TGATCGTGCG CTCTGTGCTG 
ACGAGGCCCA GGTTTAAGTG TTATCTCAGC CGACTGCACA CCTTTCTGTC TCTGTCTGGA 
CCTCACCTCG GCACACTGTA CAACAGCTCA GCACTCGTCA ATACTGGACT GTGGTTCATG 
CAGAAGTGGA AGAAGTCTGG CTCTCTGCTG CAGCTGACCT GTCGAGACCA TTCAGACCCG 
CGTCAGACCT TTCTCTATAA GCTGAGCAAG AAAGCAGGCC TTCAGTTCTT CAAGAATGTG 
GTGTTGGTGG GCTCTCTTCA GGACCGATAC GTTCCTTATC ACTCCGCTCG AATCGAGATG 
TGCAAAACAG CCTTAAAGGA CAAACAGACT GGTCCGGTGT ATGCTGAGAT GATCGAGAAC 
CTTCTCCTTC CTGTGCTTCA GAACAAAGAC TGTAACCTGG TGCGTTACGA CGTCATCCAC 
GCGCTGCCCA ACACCGCCAA CTCTCTGATC GGCCGCGCAG CTCACATCGC CGTCCTGGAC 
TCTGAGATTT TCCTGGAAAA GTTCTTCCTG GTGGCAGGGC TCAAGTTTTT TCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139066.1
CDS	1..4017
Translation	MTEVQATVEFSVELHKFYNVDLFQRGFYQVRASMKVPPRIPQRVEASLLHPIGSDLAFPASVQDDVVCSKTFQILYKNEEIVVNDVFVFKVMMLLDAKKVEESLNEVDFQLCLDLFFTDGDYSPEEPSSLQSISSRTLRLHFSLHRGIHHHVNVMFDYFHLSVISATIHASLVALHQPLISFPRPVKSTWLNRNAPAQSKDSLIPPLETVVFGSTYVKQLSPDGKSFLVSDQCLQHAFSLHQNMCANLLRAYQSLFCYLTSLTKNLPASQRMELAKPSMQVLYERVLRRPYPPGQRHVYIEELDVEVRLHELCEQVKQKAESPDELAELVNMNLAQLCSLLMALWGNFLEVVSLNERVFVLLAQEHHTLRVQRFAEAFFCLEHPRQSALAYQELHAHSHQQMTTAIKSSSYFLSLPPLPVECADLDGDVNSLPIIFEDRYLESVTEDRDGPWLGLQNNRSGSVSAKADRSNSKDCIASGALGPYPDKQQASFATAWPEQNEPSLKSKAKSIKLKKNTKTENSKKLIRQSSKDSVVLTGYKNLKVPHSEKTIKSKDIDHQCNQRDELEDGGKFLLQNETTTTHCAQNAKDCTSGNLQGNATILSDGFPNLMSLQMQARPQAGTGAEAAAARVDPTHPPAGLRHIDVKPSDKDPYQGDKVTVLLPHHPERTTKRDVPEITQTDFSEFKSRVGDGATFVGPCGAALRNDNIPQASSRLSDSGIESEPSSATQLAPVSPSPFAPIGIEAPTELIPHRPPPLSPASRGATALEGLNPESTSGVSGVQSSLTSINSLPSDDEVETEATSRKSSILVQEQSLIFSGESQSLLLTNSRVLAQQKASHGDGMDSALQSRCETSASDRLVGSNETDLPGGPEACSEPQTAAQSSTSVTSSTDMVKKGFVENYFGSRSSTDISEISPLETAAITLGIQTCPNANDEEDEEEEQEHEMIENGYYEETDGVVYVNGLPEDEGRCEDGGAEGRTLFYDHLGLEQLQETYIPSAGIRTRPTGPAPTSSDLCWYKSAPTAQMTAFVQAKEELKQLKLPGFLYSEVADLASTVPYFSMDEDENCDDGIHLIVCVHGLDGNSADLRLVKTYLELGLPGARIDFLMSERNQNDTFADFESMTDRLLDEIVQYIQIYNLTVSKISFVGHSLGNLIVRSVLTRPRFKCYLSRLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSKKAGLQFFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQTGPVYAEMIENLLLPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEIFLEKFFLVAGLKFFQ

Target ORF information:

RefSeq Version	NM_001145594.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio family with sequence similarity 135 member A (fam135a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145594.1

ORF Insert Sequence:

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGTA 
GAAGAGTCTT TAAATGAAGT TGACTTTCAG CTGTGTTTGG ACCTGTTCTT CACTGATGGA 
GACTACTCGC CTGAGGAGCC CAGTTCTCTG CAGAGCATCA GCAGCCGTAC GCTGCGTTTG 
CACTTTAGCC TCCACAGAGG CATCCACCAC CACGTCAACG TCATGTTTGA CTACTTCCAC 
CTGTCTGTCA TCTCTGCCAC AATCCATGCT TCACTGGTGG CTCTGCATCA GCCTCTAATA 
AGTTTCCCCC GGCCAGTAAA AAGCACCTGG TTGAATCGTA ATGCTCCGGC ACAGAGCAAA 
GACTCCCTCA TCCCACCGCT GGAGACTGTG GTGTTCGGCA GCACCTACGT CAAGCAGCTG 
TCCCCTGATG GCAAAAGCTT CCTGGTATCG GATCAGTGCC TCCAGCATGC CTTCAGCCTG 
CACCAGAACA TGTGTGCCAA CCTGCTGCGG GCATATCAGA GCCTGTTCTG TTACTTAACC 
TCCCTGACCA AGAATCTGCC CGCATCCCAG CGCATGGAGC TTGCCAAGCC CAGCATGCAG 
GTCCTTTATG AACGAGTTCT CAGAAGACCT TACCCTCCAG GCCAAAGGCA CGTCTACATA 
GAGGAACTTG ATGTGGAGGT CCGACTTCAT GAGCTTTGTG AACAAGTCAA GCAGAAAGCA 
GAGAGCCCAG ATGAGTTGGC TGAATTGGTG AACATGAATT TGGCGCAACT ATGTTCCCTA 
CTCATGGCTT TGTGGGGGAA TTTCCTGGAA GTTGTGTCAC TAAATGAGCG TGTTTTTGTG 
CTTTTGGCCC AGGAGCACCA CACACTACGG GTGCAAAGAT TTGCTGAAGC TTTCTTTTGT 
CTTGAACACC CACGACAGAG TGCACTGGCC TACCAGGAAC TGCATGCTCA CAGTCACCAA 
CAAATGACCA CAGCTATCAA AAGCTCTTCC TACTTCTTGT CCTTACCACC TCTTCCTGTT 
GAATGTGCTG ATCTTGACGG GGATGTGAAC TCCCTACCAA TCATCTTTGA GGACAGATAT 
TTGGAGTCTG TTACTGAAGA TCGTGATGGT CCATGGCTTG GACTCCAAAA CAACAGGAGT 
GGCTCTGTGT CCGCCAAAGC AGATAGATCC AACTCAAAGG ACTGCATTGC CAGTGGAGCC 
CTCGGTCCGT ATCCAGATAA GCAGCAAGCT TCCTTTGCCA CTGCTTGGCC TGAACAAAAC 
GAGCCTTCCC TTAAATCCAA AGCCAAGTCC ATCAAGCTCA AGAAGAACAC CAAGACCGAA 
AACTCAAAGA AGCTGATCAG ACAGAGCTCG AAAGATTCTG TGGTGCTCAC AGGCTACAAG 
AACTTGAAGG TTCCTCACAG TGAAAAGACC ATTAAGTCCA AAGACATCGA CCATCAATGC 
AATCAGAGAG ATGAGCTAGA GGATGGAGGT AAATTCCTTC TTCAGAATGA AACCACGACC 
ACGCATTGTG CTCAGAATGC TAAAGACTGC ACATCTGGCA ACCTTCAAGG TAACGCCACA 
ATCCTAAGTG ACGGCTTTCC CAATCTTATG AGTTTGCAAA TGCAGGCCAG GCCCCAGGCA 
GGTACTGGAG CAGAGGCCGC CGCTGCCAGA GTAGATCCCA CACATCCACC TGCTGGCCTC 
AGACATATTG ATGTCAAACC CAGTGACAAG GACCCCTACC AGGGCGATAA GGTCACCGTT 
CTCTTACCGC ACCACCCAGA GCGCACCACC AAGAGGGATG TTCCAGAAAT CACACAGACT 
GATTTCTCTG AGTTCAAGAG TCGAGTTGGA GATGGTGCTA CTTTCGTCGG ACCTTGTGGA 
GCGGCTTTGA GGAACGATAA TATTCCTCAA GCCTCCTCCA GACTCTCGGA TTCCGGAATT 
GAAAGCGAAC CCAGCTCTGC GACACAGCTA GCCCCGGTTT CTCCATCTCC TTTTGCTCCT 
ATTGGGATCG AAGCACCTAC AGAGCTGATT CCTCACAGAC CACCTCCGCT CAGTCCTGCT 
TCCAGAGGAG CCACCGCATT GGAAGGCCTC AATCCTGAAA GCACCAGCGG CGTAAGCGGC 
GTCCAATCTT CCCTCACCTC CATCAACTCA CTCCCTTCGG ATGATGAGGT GGAGACAGAG 
GCCACCAGCA GGAAGTCCAG CATCCTGGTC CAGGAACAGA GTCTCATCTT CTCCGGAGAA 
TCTCAAAGCC TCTTGCTGAC AAACTCTAGA GTCTTGGCTC AACAAAAAGC CAGTCATGGA 
GATGGGATGG ACTCTGCTTT GCAGTCCCGC TGTGAAACTT CTGCTTCTGA CAGACTTGTC 
GGATCTAACG AGACAGATCT TCCAGGTGGT CCGGAAGCTT GTAGTGAACC ACAGACAGCA 
GCCCAGTCCA GTACATCAGT CACCAGCAGC ACAGACATGG TGAAGAAAGG CTTTGTAGAA 
AACTACTTTG GCTCTCGGTC CAGTACTGAT ATATCTGAAA TCAGCCCTTT AGAGACGGCA 
GCCATAACAT TGGGTATTCA GACATGCCCC AATGCTAATG ATGAAGAGGA TGAGGAGGAA 
GAGCAGGAGC ATGAGATGAT TGAGAATGGT TATTATGAGG AGACCGATGG TGTGGTTTAT 
GTTAATGGGC TCCCGGAGGA TGAAGGGAGG TGTGAAGATG GAGGAGCGGA GGGAAGAACA 
CTGTTTTATG ATCATCTTGG TTTAGAGCAG CTCCAGGAAA CTTACATTCC CTCTGCCGGC 
ATTCGGACAC GGCCTACTGG ACCAGCACCA ACCAGCAGCG ACTTATGCTG GTACAAGAGC 
GCCCCCACTG CTCAGATGAC GGCTTTTGTC CAAGCGAAAG AGGAGTTGAA ACAACTAAAG 
CTGCCAGGCT TCCTGTACAG TGAGGTGGCA GATCTGGCAT CCACTGTGCC ATATTTCAGC 
ATGGATGAGG ATGAAAACTG TGATGATGGC ATACACCTTA TAGTGTGTGT ACACGGCTTG 
GATGGTAACA GTGCAGATTT GCGCCTGGTG AAGACTTATC TGGAGCTTGG GTTGCCTGGA 
GCTAGGATAG ATTTTCTGAT GTCTGAGCGA AATCAGAATG ACACATTTGC TGATTTTGAG 
TCCATGACCG ACAGACTTTT GGATGAAATT GTGCAGTACA TACAAATCTA CAACCTCACA 
GTTTCCAAGA TCAGTTTTGT TGGTCATTCT CTGGGAAATC TGATCGTGCG CTCTGTGCTG 
ACGAGGCCCA GGTTTAAGTG TTATCTCAGC CGACTGCACA CCTTTCTGTC TCTGTCTGGA 
CCTCACCTCG GCACACTGTA CAACAGCTCA GCACTCGTCA ATACTGGACT GTGGTTCATG 
CAGAAGTGGA AGAAGTCTGG CTCTCTGCTG CAGCTGACCT GTCGAGACCA TTCAGACCCG 
CGTCAGACCT TTCTCTATAA GCTGAGCAAG AAAGCAGGCC TTCAGTTCTT CAAGAATGTG 
GTGTTGGTGG GCTCTCTTCA GGACCGATAC GTTCCTTATC ACTCCGCTCG AATCGAGATG 
TGCAAAACAG CCTTAAAGGA CAAACAGACT GGTCCGGTGT ATGCTGAGAT GATCGAGAAC 
CTTCTCCTTC CTGTGCTTCA GAACAAAGAC TGTAACCTGG TGCGTTACGA CGTCATCCAC 
GCGCTGCCCA ACACCGCCAA CTCTCTGATC GGCCGCGCAG CTCACATCGC CGTCCTGGAC 
TCTGAGATTT TCCTGGAAAA GTTCTTCCTG GTGGCAGGGC TCAAGTTTTT TCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam135a ( XM_005157006.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157063.1 Danio rerio family with sequence similarity 135, member A (fam135a), transcript variant X3, mRNA.

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CloneID	ODa18993
Clone ID Related Accession (Same CDS sequence)	XM_005157006.4 , NM_001145594.1 , XM_005157006.3
Accession Version	XM_005157006.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein FAM135A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157006.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGTA 
GAAGAGTCTT TAAATGAAGT TGACTTTCAG CTGTGTTTGG ACCTGTTCTT CACTGATGGA 
GACTACTCGC CTGAGGAGCC CAGTTCTCTG CAGAGCATCA GCAGCCGTAC GCTGCGTTTG 
CACTTTAGCC TCCACAGAGG CATCCACCAC CACGTCAACG TCATGTTTGA CTACTTCCAC 
CTGTCTGTCA TCTCTGCCAC AATCCATGCT TCACTGGTGG CTCTGCATCA GCCTCTAATA 
AGTTTCCCCC GGCCAGTAAA AAGCACCTGG TTGAATCGTA ATGCTCCGGC ACAGAGCAAA 
GACTCCCTCA TCCCACCGCT GGAGACTGTG GTGTTCGGCA GCACCTACGT CAAGCAGCTG 
TCCCCTGATG GCAAAAGCTT CCTGGTATCG GATCAGTGCC TCCAGCATGC CTTCAGCCTG 
CACCAGAACA TGTGTGCCAA CCTGCTGCGG GCATATCAGA GCCTGTTCTG TTACTTAACC 
TCCCTGACCA AGAATCTGCC CGCATCCCAG CGCATGGAGC TTGCCAAGCC CAGCATGCAG 
GTCCTTTATG AACGAGTTCT CAGAAGACCT TACCCTCCAG GCCAAAGGCA CGTCTACATA 
GAGGAACTTG ATGTGGAGGT CCGACTTCAT GAGCTTTGTG AACAAGTCAA GCAGAAAGCA 
GAGAGCCCAG ATGAGTTGGC TGAATTGGTG AACATGAATT TGGCGCAACT ATGTTCCCTA 
CTCATGGCTT TGTGGGGGAA TTTCCTGGAA GTTGTGTCAC TAAATGAGCG TGTTTTTGTG 
CTTTTGGCCC AGGAGCACCA CACACTACGG GTGCAAAGAT TTGCTGAAGC TTTCTTTTGT 
CTTGAACACC CACGACAGAG TGCACTGGCC TACCAGGAAC TGCATGCTCA CAGTCACCAA 
CAAATGACCA CAGCTATCAA AAGCTCTTCC TACTTCTTGT CCTTACCACC TCTTCCTGTT 
GAATGTGCTG ATCTTGACGG GGATGTGAAC TCCCTACCAA TCATCTTTGA GGACAGATAT 
TTGGAGTCTG TTACTGAAGA TCGTGATGGT CCATGGCTTG GACTCCAAAA CAACAGGAGT 
GGCTCTGTGT CCGCCAAAGC AGATAGATCC AACTCAAAGG ACTGCATTGC CAGTGGAGCC 
CTCGGTCCGT ATCCAGATAA GCAGCAAGCT TCCTTTGCCA CTGCTTGGCC TGAACAAAAC 
GAGCCTTCCC TTAAATCCAA AGCCAAGTCC ATCAAGCTCA AGAAGAACAC CAAGACCGAA 
AACTCAAAGA AGCTGATCAG ACAGAGCTCG AAAGATTCTG TGGTGCTCAC AGGCTACAAG 
AACTTGAAGG TTCCTCACAG TGAAAAGACC ATTAAGTCCA AAGACATCGA CCATCAATGC 
AATCAGAGAG ATGAGCTAGA GGATGGAGGT AAATTCCTTC TTCAGAATGA AACCACGACC 
ACGCATTGTG CTCAGAATGC TAAAGACTGC ACATCTGGCA ACCTTCAAGG TAACGCCACA 
ATCCTAAGTG ACGGCTTTCC CAATCTTATG AGTTTGCAAA TGCAGGCCAG GCCCCAGGCA 
GGTACTGGAG CAGAGGCCGC CGCTGCCAGA GTAGATCCCA CACATCCACC TGCTGGCCTC 
AGACATATTG ATGTCAAACC CAGTGACAAG GACCCCTACC AGGGCGATAA GGTCACCGTT 
CTCTTACCGC ACCACCCAGA GCGCACCACC AAGAGGGATG TTCCAGAAAT CACACAGACT 
GATTTCTCTG AGTTCAAGAG TCGAGTTGGA GATGGTGCTA CTTTCGTCGG ACCTTGTGGA 
GCGGCTTTGA GGAACGATAA TATTCCTCAA GCCTCCTCCA GACTCTCGGA TTCCGGAATT 
GAAAGCGAAC CCAGCTCTGC GACACAGCTA GCCCCGGTTT CTCCATCTCC TTTTGCTCCT 
ATTGGGATCG AAGCACCTAC AGAGCTGATT CCTCACAGAC CACCTCCGCT CAGTCCTGCT 
TCCAGAGGAG CCACCGCATT GGAAGGCCTC AATCCTGAAA GCACCAGCGG CGTAAGCGGC 
GTCCAATCTT CCCTCACCTC CATCAACTCA CTCCCTTCGG ATGATGAGGT GGAGACAGAG 
GCCACCAGCA GGAAGTCCAG CATCCTGGTC CAGGAACAGA GTCTCATCTT CTCCGGAGAA 
TCTCAAAGCC TCTTGCTGAC AAACTCTAGA GTCTTGGCTC AACAAAAAGC CAGTCATGGA 
GATGGGATGG ACTCTGCTTT GCAGTCCCGC TGTGAAACTT CTGCTTCTGA CAGACTTGTC 
GGATCTAACG AGACAGATCT TCCAGGTGGT CCGGAAGCTT GTAGTGAACC ACAGACAGCA 
GCCCAGTCCA GTACATCAGT CACCAGCAGC ACAGACATGG TGAAGAAAGG CTTTGTAGAA 
AACTACTTTG GCTCTCGGTC CAGTACTGAT ATATCTGAAA TCAGCCCTTT AGAGACGGCA 
GCCATAACAT TGGGTATTCA GACATGCCCC AATGCTAATG ATGAAGAGGA TGAGGAGGAA 
GAGCAGGAGC ATGAGATGAT TGAGAATGGT TATTATGAGG AGACCGATGG TGTGGTTTAT 
GTTAATGGGC TCCCGGAGGA TGAAGGGAGG TGTGAAGATG GAGGAGCGGA GGGAAGAACA 
CTGTTTTATG ATCATCTTGG TTTAGAGCAG CTCCAGGAAA CTTACATTCC CTCTGCCGGC 
ATTCGGACAC GGCCTACTGG ACCAGCACCA ACCAGCAGCG ACTTATGCTG GTACAAGAGC 
GCCCCCACTG CTCAGATGAC GGCTTTTGTC CAAGCGAAAG AGGAGTTGAA ACAACTAAAG 
CTGCCAGGCT TCCTGTACAG TGAGGTGGCA GATCTGGCAT CCACTGTGCC ATATTTCAGC 
ATGGATGAGG ATGAAAACTG TGATGATGGC ATACACCTTA TAGTGTGTGT ACACGGCTTG 
GATGGTAACA GTGCAGATTT GCGCCTGGTG AAGACTTATC TGGAGCTTGG GTTGCCTGGA 
GCTAGGATAG ATTTTCTGAT GTCTGAGCGA AATCAGAATG ACACATTTGC TGATTTTGAG 
TCCATGACCG ACAGACTTTT GGATGAAATT GTGCAGTACA TACAAATCTA CAACCTCACA 
GTTTCCAAGA TCAGTTTTGT TGGTCATTCT CTGGGAAATC TGATCGTGCG CTCTGTGCTG 
ACGAGGCCCA GGTTTAAGTG TTATCTCAGC CGACTGCACA CCTTTCTGTC TCTGTCTGGA 
CCTCACCTCG GCACACTGTA CAACAGCTCA GCACTCGTCA ATACTGGACT GTGGTTCATG 
CAGAAGTGGA AGAAGTCTGG CTCTCTGCTG CAGCTGACCT GTCGAGACCA TTCAGACCCG 
CGTCAGACCT TTCTCTATAA GCTGAGCAAG AAAGCAGGCC TTCAGTTCTT CAAGAATGTG 
GTGTTGGTGG GCTCTCTTCA GGACCGATAC GTTCCTTATC ACTCCGCTCG AATCGAGATG 
TGCAAAACAG CCTTAAAGGA CAAACAGACT GGTCCGGTGT ATGCTGAGAT GATCGAGAAC 
CTTCTCCTTC CTGTGCTTCA GAACAAAGAC TGTAACCTGG TGCGTTACGA CGTCATCCAC 
GCGCTGCCCA ACACCGCCAA CTCTCTGATC GGCCGCGCAG CTCACATCGC CGTCCTGGAC 
TCTGAGATTT TCCTGGAAAA GTTCTTCCTG GTGGCAGGGC TCAAGTTTTT TCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157063.1
CDS	405..4421
Translation	MTEVQATVEFSVELHKFYNVDLFQRGFYQVRASMKVPPRIPQRVEASLLHPIGSDLAFPASVQDDVVCSKTFQILYKNEEIVVNDVFVFKVMMLLDAKKVEESLNEVDFQLCLDLFFTDGDYSPEEPSSLQSISSRTLRLHFSLHRGIHHHVNVMFDYFHLSVISATIHASLVALHQPLISFPRPVKSTWLNRNAPAQSKDSLIPPLETVVFGSTYVKQLSPDGKSFLVSDQCLQHAFSLHQNMCANLLRAYQSLFCYLTSLTKNLPASQRMELAKPSMQVLYERVLRRPYPPGQRHVYIEELDVEVRLHELCEQVKQKAESPDELAELVNMNLAQLCSLLMALWGNFLEVVSLNERVFVLLAQEHHTLRVQRFAEAFFCLEHPRQSALAYQELHAHSHQQMTTAIKSSSYFLSLPPLPVECADLDGDVNSLPIIFEDRYLESVTEDRDGPWLGLQNNRSGSVSAKADRSNSKDCIASGALGPYPDKQQASFATAWPEQNEPSLKSKAKSIKLKKNTKTENSKKLIRQSSKDSVVLTGYKNLKVPHSEKTIKSKDIDHQCNQRDELEDGGKFLLQNETTTTHCAQNAKDCTSGNLQGNATILSDGFPNLMSLQMQARPQAGTGAEAAAARVDPTHPPAGLRHIDVKPSDKDPYQGDKVTVLLPHHPERTTKRDVPEITQTDFSEFKSRVGDGATFVGPCGAALRNDNIPQASSRLSDSGIESEPSSATQLAPVSPSPFAPIGIEAPTELIPHRPPPLSPASRGATALEGLNPESTSGVSGVQSSLTSINSLPSDDEVETEATSRKSSILVQEQSLIFSGESQSLLLTNSRVLAQQKASHGDGMDSALQSRCETSASDRLVGSNETDLPGGPEACSEPQTAAQSSTSVTSSTDMVKKGFVENYFGSRSSTDISEISPLETAAITLGIQTCPNANDEEDEEEEQEHEMIENGYYEETDGVVYVNGLPEDEGRCEDGGAEGRTLFYDHLGLEQLQETYIPSAGIRTRPTGPAPTSSDLCWYKSAPTAQMTAFVQAKEELKQLKLPGFLYSEVADLASTVPYFSMDEDENCDDGIHLIVCVHGLDGNSADLRLVKTYLELGLPGARIDFLMSERNQNDTFADFESMTDRLLDEIVQYIQIYNLTVSKISFVGHSLGNLIVRSVLTRPRFKCYLSRLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSKKAGLQFFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQTGPVYAEMIENLLLPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEIFLEKFFLVAGLKFFQ

Target ORF information:

RefSeq Version	XM_005157006.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio family with sequence similarity 135, member A (fam135a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157006.4

ORF Insert Sequence:

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGTA 
GAAGAGTCTT TAAATGAAGT TGACTTTCAG CTGTGTTTGG ACCTGTTCTT CACTGATGGA 
GACTACTCGC CTGAGGAGCC CAGTTCTCTG CAGAGCATCA GCAGCCGTAC GCTGCGTTTG 
CACTTTAGCC TCCACAGAGG CATCCACCAC CACGTCAACG TCATGTTTGA CTACTTCCAC 
CTGTCTGTCA TCTCTGCCAC AATCCATGCT TCACTGGTGG CTCTGCATCA GCCTCTAATA 
AGTTTCCCCC GGCCAGTAAA AAGCACCTGG TTGAATCGTA ATGCTCCGGC ACAGAGCAAA 
GACTCCCTCA TCCCACCGCT GGAGACTGTG GTGTTCGGCA GCACCTACGT CAAGCAGCTG 
TCCCCTGATG GCAAAAGCTT CCTGGTATCG GATCAGTGCC TCCAGCATGC CTTCAGCCTG 
CACCAGAACA TGTGTGCCAA CCTGCTGCGG GCATATCAGA GCCTGTTCTG TTACTTAACC 
TCCCTGACCA AGAATCTGCC CGCATCCCAG CGCATGGAGC TTGCCAAGCC CAGCATGCAG 
GTCCTTTATG AACGAGTTCT CAGAAGACCT TACCCTCCAG GCCAAAGGCA CGTCTACATA 
GAGGAACTTG ATGTGGAGGT CCGACTTCAT GAGCTTTGTG AACAAGTCAA GCAGAAAGCA 
GAGAGCCCAG ATGAGTTGGC TGAATTGGTG AACATGAATT TGGCGCAACT ATGTTCCCTA 
CTCATGGCTT TGTGGGGGAA TTTCCTGGAA GTTGTGTCAC TAAATGAGCG TGTTTTTGTG 
CTTTTGGCCC AGGAGCACCA CACACTACGG GTGCAAAGAT TTGCTGAAGC TTTCTTTTGT 
CTTGAACACC CACGACAGAG TGCACTGGCC TACCAGGAAC TGCATGCTCA CAGTCACCAA 
CAAATGACCA CAGCTATCAA AAGCTCTTCC TACTTCTTGT CCTTACCACC TCTTCCTGTT 
GAATGTGCTG ATCTTGACGG GGATGTGAAC TCCCTACCAA TCATCTTTGA GGACAGATAT 
TTGGAGTCTG TTACTGAAGA TCGTGATGGT CCATGGCTTG GACTCCAAAA CAACAGGAGT 
GGCTCTGTGT CCGCCAAAGC AGATAGATCC AACTCAAAGG ACTGCATTGC CAGTGGAGCC 
CTCGGTCCGT ATCCAGATAA GCAGCAAGCT TCCTTTGCCA CTGCTTGGCC TGAACAAAAC 
GAGCCTTCCC TTAAATCCAA AGCCAAGTCC ATCAAGCTCA AGAAGAACAC CAAGACCGAA 
AACTCAAAGA AGCTGATCAG ACAGAGCTCG AAAGATTCTG TGGTGCTCAC AGGCTACAAG 
AACTTGAAGG TTCCTCACAG TGAAAAGACC ATTAAGTCCA AAGACATCGA CCATCAATGC 
AATCAGAGAG ATGAGCTAGA GGATGGAGGT AAATTCCTTC TTCAGAATGA AACCACGACC 
ACGCATTGTG CTCAGAATGC TAAAGACTGC ACATCTGGCA ACCTTCAAGG TAACGCCACA 
ATCCTAAGTG ACGGCTTTCC CAATCTTATG AGTTTGCAAA TGCAGGCCAG GCCCCAGGCA 
GGTACTGGAG CAGAGGCCGC CGCTGCCAGA GTAGATCCCA CACATCCACC TGCTGGCCTC 
AGACATATTG ATGTCAAACC CAGTGACAAG GACCCCTACC AGGGCGATAA GGTCACCGTT 
CTCTTACCGC ACCACCCAGA GCGCACCACC AAGAGGGATG TTCCAGAAAT CACACAGACT 
GATTTCTCTG AGTTCAAGAG TCGAGTTGGA GATGGTGCTA CTTTCGTCGG ACCTTGTGGA 
GCGGCTTTGA GGAACGATAA TATTCCTCAA GCCTCCTCCA GACTCTCGGA TTCCGGAATT 
GAAAGCGAAC CCAGCTCTGC GACACAGCTA GCCCCGGTTT CTCCATCTCC TTTTGCTCCT 
ATTGGGATCG AAGCACCTAC AGAGCTGATT CCTCACAGAC CACCTCCGCT CAGTCCTGCT 
TCCAGAGGAG CCACCGCATT GGAAGGCCTC AATCCTGAAA GCACCAGCGG CGTAAGCGGC 
GTCCAATCTT CCCTCACCTC CATCAACTCA CTCCCTTCGG ATGATGAGGT GGAGACAGAG 
GCCACCAGCA GGAAGTCCAG CATCCTGGTC CAGGAACAGA GTCTCATCTT CTCCGGAGAA 
TCTCAAAGCC TCTTGCTGAC AAACTCTAGA GTCTTGGCTC AACAAAAAGC CAGTCATGGA 
GATGGGATGG ACTCTGCTTT GCAGTCCCGC TGTGAAACTT CTGCTTCTGA CAGACTTGTC 
GGATCTAACG AGACAGATCT TCCAGGTGGT CCGGAAGCTT GTAGTGAACC ACAGACAGCA 
GCCCAGTCCA GTACATCAGT CACCAGCAGC ACAGACATGG TGAAGAAAGG CTTTGTAGAA 
AACTACTTTG GCTCTCGGTC CAGTACTGAT ATATCTGAAA TCAGCCCTTT AGAGACGGCA 
GCCATAACAT TGGGTATTCA GACATGCCCC AATGCTAATG ATGAAGAGGA TGAGGAGGAA 
GAGCAGGAGC ATGAGATGAT TGAGAATGGT TATTATGAGG AGACCGATGG TGTGGTTTAT 
GTTAATGGGC TCCCGGAGGA TGAAGGGAGG TGTGAAGATG GAGGAGCGGA GGGAAGAACA 
CTGTTTTATG ATCATCTTGG TTTAGAGCAG CTCCAGGAAA CTTACATTCC CTCTGCCGGC 
ATTCGGACAC GGCCTACTGG ACCAGCACCA ACCAGCAGCG ACTTATGCTG GTACAAGAGC 
GCCCCCACTG CTCAGATGAC GGCTTTTGTC CAAGCGAAAG AGGAGTTGAA ACAACTAAAG 
CTGCCAGGCT TCCTGTACAG TGAGGTGGCA GATCTGGCAT CCACTGTGCC ATATTTCAGC 
ATGGATGAGG ATGAAAACTG TGATGATGGC ATACACCTTA TAGTGTGTGT ACACGGCTTG 
GATGGTAACA GTGCAGATTT GCGCCTGGTG AAGACTTATC TGGAGCTTGG GTTGCCTGGA 
GCTAGGATAG ATTTTCTGAT GTCTGAGCGA AATCAGAATG ACACATTTGC TGATTTTGAG 
TCCATGACCG ACAGACTTTT GGATGAAATT GTGCAGTACA TACAAATCTA CAACCTCACA 
GTTTCCAAGA TCAGTTTTGT TGGTCATTCT CTGGGAAATC TGATCGTGCG CTCTGTGCTG 
ACGAGGCCCA GGTTTAAGTG TTATCTCAGC CGACTGCACA CCTTTCTGTC TCTGTCTGGA 
CCTCACCTCG GCACACTGTA CAACAGCTCA GCACTCGTCA ATACTGGACT GTGGTTCATG 
CAGAAGTGGA AGAAGTCTGG CTCTCTGCTG CAGCTGACCT GTCGAGACCA TTCAGACCCG 
CGTCAGACCT TTCTCTATAA GCTGAGCAAG AAAGCAGGCC TTCAGTTCTT CAAGAATGTG 
GTGTTGGTGG GCTCTCTTCA GGACCGATAC GTTCCTTATC ACTCCGCTCG AATCGAGATG 
TGCAAAACAG CCTTAAAGGA CAAACAGACT GGTCCGGTGT ATGCTGAGAT GATCGAGAAC 
CTTCTCCTTC CTGTGCTTCA GAACAAAGAC TGTAACCTGG TGCGTTACGA CGTCATCCAC 
GCGCTGCCCA ACACCGCCAA CTCTCTGATC GGCCGCGCAG CTCACATCGC CGTCCTGGAC 
TCTGAGATTT TCCTGGAAAA GTTCTTCCTG GTGGCAGGGC TCAAGTTTTT TCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam135a ( XM_005157006.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157063.1 Danio rerio family with sequence similarity 135 member A (fam135a), transcript variant X3, mRNA.

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CloneID	ODa18993
Clone ID Related Accession (Same CDS sequence)	XM_005157006.4 , NM_001145594.1 , XM_005157006.3
Accession Version	XM_005157006.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4017bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein FAM135A isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157006.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGTA 
GAAGAGTCTT TAAATGAAGT TGACTTTCAG CTGTGTTTGG ACCTGTTCTT CACTGATGGA 
GACTACTCGC CTGAGGAGCC CAGTTCTCTG CAGAGCATCA GCAGCCGTAC GCTGCGTTTG 
CACTTTAGCC TCCACAGAGG CATCCACCAC CACGTCAACG TCATGTTTGA CTACTTCCAC 
CTGTCTGTCA TCTCTGCCAC AATCCATGCT TCACTGGTGG CTCTGCATCA GCCTCTAATA 
AGTTTCCCCC GGCCAGTAAA AAGCACCTGG TTGAATCGTA ATGCTCCGGC ACAGAGCAAA 
GACTCCCTCA TCCCACCGCT GGAGACTGTG GTGTTCGGCA GCACCTACGT CAAGCAGCTG 
TCCCCTGATG GCAAAAGCTT CCTGGTATCG GATCAGTGCC TCCAGCATGC CTTCAGCCTG 
CACCAGAACA TGTGTGCCAA CCTGCTGCGG GCATATCAGA GCCTGTTCTG TTACTTAACC 
TCCCTGACCA AGAATCTGCC CGCATCCCAG CGCATGGAGC TTGCCAAGCC CAGCATGCAG 
GTCCTTTATG AACGAGTTCT CAGAAGACCT TACCCTCCAG GCCAAAGGCA CGTCTACATA 
GAGGAACTTG ATGTGGAGGT CCGACTTCAT GAGCTTTGTG AACAAGTCAA GCAGAAAGCA 
GAGAGCCCAG ATGAGTTGGC TGAATTGGTG AACATGAATT TGGCGCAACT ATGTTCCCTA 
CTCATGGCTT TGTGGGGGAA TTTCCTGGAA GTTGTGTCAC TAAATGAGCG TGTTTTTGTG 
CTTTTGGCCC AGGAGCACCA CACACTACGG GTGCAAAGAT TTGCTGAAGC TTTCTTTTGT 
CTTGAACACC CACGACAGAG TGCACTGGCC TACCAGGAAC TGCATGCTCA CAGTCACCAA 
CAAATGACCA CAGCTATCAA AAGCTCTTCC TACTTCTTGT CCTTACCACC TCTTCCTGTT 
GAATGTGCTG ATCTTGACGG GGATGTGAAC TCCCTACCAA TCATCTTTGA GGACAGATAT 
TTGGAGTCTG TTACTGAAGA TCGTGATGGT CCATGGCTTG GACTCCAAAA CAACAGGAGT 
GGCTCTGTGT CCGCCAAAGC AGATAGATCC AACTCAAAGG ACTGCATTGC CAGTGGAGCC 
CTCGGTCCGT ATCCAGATAA GCAGCAAGCT TCCTTTGCCA CTGCTTGGCC TGAACAAAAC 
GAGCCTTCCC TTAAATCCAA AGCCAAGTCC ATCAAGCTCA AGAAGAACAC CAAGACCGAA 
AACTCAAAGA AGCTGATCAG ACAGAGCTCG AAAGATTCTG TGGTGCTCAC AGGCTACAAG 
AACTTGAAGG TTCCTCACAG TGAAAAGACC ATTAAGTCCA AAGACATCGA CCATCAATGC 
AATCAGAGAG ATGAGCTAGA GGATGGAGGT AAATTCCTTC TTCAGAATGA AACCACGACC 
ACGCATTGTG CTCAGAATGC TAAAGACTGC ACATCTGGCA ACCTTCAAGG TAACGCCACA 
ATCCTAAGTG ACGGCTTTCC CAATCTTATG AGTTTGCAAA TGCAGGCCAG GCCCCAGGCA 
GGTACTGGAG CAGAGGCCGC CGCTGCCAGA GTAGATCCCA CACATCCACC TGCTGGCCTC 
AGACATATTG ATGTCAAACC CAGTGACAAG GACCCCTACC AGGGCGATAA GGTCACCGTT 
CTCTTACCGC ACCACCCAGA GCGCACCACC AAGAGGGATG TTCCAGAAAT CACACAGACT 
GATTTCTCTG AGTTCAAGAG TCGAGTTGGA GATGGTGCTA CTTTCGTCGG ACCTTGTGGA 
GCGGCTTTGA GGAACGATAA TATTCCTCAA GCCTCCTCCA GACTCTCGGA TTCCGGAATT 
GAAAGCGAAC CCAGCTCTGC GACACAGCTA GCCCCGGTTT CTCCATCTCC TTTTGCTCCT 
ATTGGGATCG AAGCACCTAC AGAGCTGATT CCTCACAGAC CACCTCCGCT CAGTCCTGCT 
TCCAGAGGAG CCACCGCATT GGAAGGCCTC AATCCTGAAA GCACCAGCGG CGTAAGCGGC 
GTCCAATCTT CCCTCACCTC CATCAACTCA CTCCCTTCGG ATGATGAGGT GGAGACAGAG 
GCCACCAGCA GGAAGTCCAG CATCCTGGTC CAGGAACAGA GTCTCATCTT CTCCGGAGAA 
TCTCAAAGCC TCTTGCTGAC AAACTCTAGA GTCTTGGCTC AACAAAAAGC CAGTCATGGA 
GATGGGATGG ACTCTGCTTT GCAGTCCCGC TGTGAAACTT CTGCTTCTGA CAGACTTGTC 
GGATCTAACG AGACAGATCT TCCAGGTGGT CCGGAAGCTT GTAGTGAACC ACAGACAGCA 
GCCCAGTCCA GTACATCAGT CACCAGCAGC ACAGACATGG TGAAGAAAGG CTTTGTAGAA 
AACTACTTTG GCTCTCGGTC CAGTACTGAT ATATCTGAAA TCAGCCCTTT AGAGACGGCA 
GCCATAACAT TGGGTATTCA GACATGCCCC AATGCTAATG ATGAAGAGGA TGAGGAGGAA 
GAGCAGGAGC ATGAGATGAT TGAGAATGGT TATTATGAGG AGACCGATGG TGTGGTTTAT 
GTTAATGGGC TCCCGGAGGA TGAAGGGAGG TGTGAAGATG GAGGAGCGGA GGGAAGAACA 
CTGTTTTATG ATCATCTTGG TTTAGAGCAG CTCCAGGAAA CTTACATTCC CTCTGCCGGC 
ATTCGGACAC GGCCTACTGG ACCAGCACCA ACCAGCAGCG ACTTATGCTG GTACAAGAGC 
GCCCCCACTG CTCAGATGAC GGCTTTTGTC CAAGCGAAAG AGGAGTTGAA ACAACTAAAG 
CTGCCAGGCT TCCTGTACAG TGAGGTGGCA GATCTGGCAT CCACTGTGCC ATATTTCAGC 
ATGGATGAGG ATGAAAACTG TGATGATGGC ATACACCTTA TAGTGTGTGT ACACGGCTTG 
GATGGTAACA GTGCAGATTT GCGCCTGGTG AAGACTTATC TGGAGCTTGG GTTGCCTGGA 
GCTAGGATAG ATTTTCTGAT GTCTGAGCGA AATCAGAATG ACACATTTGC TGATTTTGAG 
TCCATGACCG ACAGACTTTT GGATGAAATT GTGCAGTACA TACAAATCTA CAACCTCACA 
GTTTCCAAGA TCAGTTTTGT TGGTCATTCT CTGGGAAATC TGATCGTGCG CTCTGTGCTG 
ACGAGGCCCA GGTTTAAGTG TTATCTCAGC CGACTGCACA CCTTTCTGTC TCTGTCTGGA 
CCTCACCTCG GCACACTGTA CAACAGCTCA GCACTCGTCA ATACTGGACT GTGGTTCATG 
CAGAAGTGGA AGAAGTCTGG CTCTCTGCTG CAGCTGACCT GTCGAGACCA TTCAGACCCG 
CGTCAGACCT TTCTCTATAA GCTGAGCAAG AAAGCAGGCC TTCAGTTCTT CAAGAATGTG 
GTGTTGGTGG GCTCTCTTCA GGACCGATAC GTTCCTTATC ACTCCGCTCG AATCGAGATG 
TGCAAAACAG CCTTAAAGGA CAAACAGACT GGTCCGGTGT ATGCTGAGAT GATCGAGAAC 
CTTCTCCTTC CTGTGCTTCA GAACAAAGAC TGTAACCTGG TGCGTTACGA CGTCATCCAC 
GCGCTGCCCA ACACCGCCAA CTCTCTGATC GGCCGCGCAG CTCACATCGC CGTCCTGGAC 
TCTGAGATTT TCCTGGAAAA GTTCTTCCTG GTGGCAGGGC TCAAGTTTTT TCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157063.1
CDS	388..4404
Translation	MTEVQATVEFSVELHKFYNVDLFQRGFYQVRASMKVPPRIPQRVEASLLHPIGSDLAFPASVQDDVVCSKTFQILYKNEEIVVNDVFVFKVMMLLDAKKVEESLNEVDFQLCLDLFFTDGDYSPEEPSSLQSISSRTLRLHFSLHRGIHHHVNVMFDYFHLSVISATIHASLVALHQPLISFPRPVKSTWLNRNAPAQSKDSLIPPLETVVFGSTYVKQLSPDGKSFLVSDQCLQHAFSLHQNMCANLLRAYQSLFCYLTSLTKNLPASQRMELAKPSMQVLYERVLRRPYPPGQRHVYIEELDVEVRLHELCEQVKQKAESPDELAELVNMNLAQLCSLLMALWGNFLEVVSLNERVFVLLAQEHHTLRVQRFAEAFFCLEHPRQSALAYQELHAHSHQQMTTAIKSSSYFLSLPPLPVECADLDGDVNSLPIIFEDRYLESVTEDRDGPWLGLQNNRSGSVSAKADRSNSKDCIASGALGPYPDKQQASFATAWPEQNEPSLKSKAKSIKLKKNTKTENSKKLIRQSSKDSVVLTGYKNLKVPHSEKTIKSKDIDHQCNQRDELEDGGKFLLQNETTTTHCAQNAKDCTSGNLQGNATILSDGFPNLMSLQMQARPQAGTGAEAAAARVDPTHPPAGLRHIDVKPSDKDPYQGDKVTVLLPHHPERTTKRDVPEITQTDFSEFKSRVGDGATFVGPCGAALRNDNIPQASSRLSDSGIESEPSSATQLAPVSPSPFAPIGIEAPTELIPHRPPPLSPASRGATALEGLNPESTSGVSGVQSSLTSINSLPSDDEVETEATSRKSSILVQEQSLIFSGESQSLLLTNSRVLAQQKASHGDGMDSALQSRCETSASDRLVGSNETDLPGGPEACSEPQTAAQSSTSVTSSTDMVKKGFVENYFGSRSSTDISEISPLETAAITLGIQTCPNANDEEDEEEEQEHEMIENGYYEETDGVVYVNGLPEDEGRCEDGGAEGRTLFYDHLGLEQLQETYIPSAGIRTRPTGPAPTSSDLCWYKSAPTAQMTAFVQAKEELKQLKLPGFLYSEVADLASTVPYFSMDEDENCDDGIHLIVCVHGLDGNSADLRLVKTYLELGLPGARIDFLMSERNQNDTFADFESMTDRLLDEIVQYIQIYNLTVSKISFVGHSLGNLIVRSVLTRPRFKCYLSRLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSKKAGLQFFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQTGPVYAEMIENLLLPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEIFLEKFFLVAGLKFFQ

Target ORF information:

RefSeq Version	XM_005157006.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio family with sequence similarity 135 member A (fam135a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157006.3

ORF Insert Sequence:

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGTA 
GAAGAGTCTT TAAATGAAGT TGACTTTCAG CTGTGTTTGG ACCTGTTCTT CACTGATGGA 
GACTACTCGC CTGAGGAGCC CAGTTCTCTG CAGAGCATCA GCAGCCGTAC GCTGCGTTTG 
CACTTTAGCC TCCACAGAGG CATCCACCAC CACGTCAACG TCATGTTTGA CTACTTCCAC 
CTGTCTGTCA TCTCTGCCAC AATCCATGCT TCACTGGTGG CTCTGCATCA GCCTCTAATA 
AGTTTCCCCC GGCCAGTAAA AAGCACCTGG TTGAATCGTA ATGCTCCGGC ACAGAGCAAA 
GACTCCCTCA TCCCACCGCT GGAGACTGTG GTGTTCGGCA GCACCTACGT CAAGCAGCTG 
TCCCCTGATG GCAAAAGCTT CCTGGTATCG GATCAGTGCC TCCAGCATGC CTTCAGCCTG 
CACCAGAACA TGTGTGCCAA CCTGCTGCGG GCATATCAGA GCCTGTTCTG TTACTTAACC 
TCCCTGACCA AGAATCTGCC CGCATCCCAG CGCATGGAGC TTGCCAAGCC CAGCATGCAG 
GTCCTTTATG AACGAGTTCT CAGAAGACCT TACCCTCCAG GCCAAAGGCA CGTCTACATA 
GAGGAACTTG ATGTGGAGGT CCGACTTCAT GAGCTTTGTG AACAAGTCAA GCAGAAAGCA 
GAGAGCCCAG ATGAGTTGGC TGAATTGGTG AACATGAATT TGGCGCAACT ATGTTCCCTA 
CTCATGGCTT TGTGGGGGAA TTTCCTGGAA GTTGTGTCAC TAAATGAGCG TGTTTTTGTG 
CTTTTGGCCC AGGAGCACCA CACACTACGG GTGCAAAGAT TTGCTGAAGC TTTCTTTTGT 
CTTGAACACC CACGACAGAG TGCACTGGCC TACCAGGAAC TGCATGCTCA CAGTCACCAA 
CAAATGACCA CAGCTATCAA AAGCTCTTCC TACTTCTTGT CCTTACCACC TCTTCCTGTT 
GAATGTGCTG ATCTTGACGG GGATGTGAAC TCCCTACCAA TCATCTTTGA GGACAGATAT 
TTGGAGTCTG TTACTGAAGA TCGTGATGGT CCATGGCTTG GACTCCAAAA CAACAGGAGT 
GGCTCTGTGT CCGCCAAAGC AGATAGATCC AACTCAAAGG ACTGCATTGC CAGTGGAGCC 
CTCGGTCCGT ATCCAGATAA GCAGCAAGCT TCCTTTGCCA CTGCTTGGCC TGAACAAAAC 
GAGCCTTCCC TTAAATCCAA AGCCAAGTCC ATCAAGCTCA AGAAGAACAC CAAGACCGAA 
AACTCAAAGA AGCTGATCAG ACAGAGCTCG AAAGATTCTG TGGTGCTCAC AGGCTACAAG 
AACTTGAAGG TTCCTCACAG TGAAAAGACC ATTAAGTCCA AAGACATCGA CCATCAATGC 
AATCAGAGAG ATGAGCTAGA GGATGGAGGT AAATTCCTTC TTCAGAATGA AACCACGACC 
ACGCATTGTG CTCAGAATGC TAAAGACTGC ACATCTGGCA ACCTTCAAGG TAACGCCACA 
ATCCTAAGTG ACGGCTTTCC CAATCTTATG AGTTTGCAAA TGCAGGCCAG GCCCCAGGCA 
GGTACTGGAG CAGAGGCCGC CGCTGCCAGA GTAGATCCCA CACATCCACC TGCTGGCCTC 
AGACATATTG ATGTCAAACC CAGTGACAAG GACCCCTACC AGGGCGATAA GGTCACCGTT 
CTCTTACCGC ACCACCCAGA GCGCACCACC AAGAGGGATG TTCCAGAAAT CACACAGACT 
GATTTCTCTG AGTTCAAGAG TCGAGTTGGA GATGGTGCTA CTTTCGTCGG ACCTTGTGGA 
GCGGCTTTGA GGAACGATAA TATTCCTCAA GCCTCCTCCA GACTCTCGGA TTCCGGAATT 
GAAAGCGAAC CCAGCTCTGC GACACAGCTA GCCCCGGTTT CTCCATCTCC TTTTGCTCCT 
ATTGGGATCG AAGCACCTAC AGAGCTGATT CCTCACAGAC CACCTCCGCT CAGTCCTGCT 
TCCAGAGGAG CCACCGCATT GGAAGGCCTC AATCCTGAAA GCACCAGCGG CGTAAGCGGC 
GTCCAATCTT CCCTCACCTC CATCAACTCA CTCCCTTCGG ATGATGAGGT GGAGACAGAG 
GCCACCAGCA GGAAGTCCAG CATCCTGGTC CAGGAACAGA GTCTCATCTT CTCCGGAGAA 
TCTCAAAGCC TCTTGCTGAC AAACTCTAGA GTCTTGGCTC AACAAAAAGC CAGTCATGGA 
GATGGGATGG ACTCTGCTTT GCAGTCCCGC TGTGAAACTT CTGCTTCTGA CAGACTTGTC 
GGATCTAACG AGACAGATCT TCCAGGTGGT CCGGAAGCTT GTAGTGAACC ACAGACAGCA 
GCCCAGTCCA GTACATCAGT CACCAGCAGC ACAGACATGG TGAAGAAAGG CTTTGTAGAA 
AACTACTTTG GCTCTCGGTC CAGTACTGAT ATATCTGAAA TCAGCCCTTT AGAGACGGCA 
GCCATAACAT TGGGTATTCA GACATGCCCC AATGCTAATG ATGAAGAGGA TGAGGAGGAA 
GAGCAGGAGC ATGAGATGAT TGAGAATGGT TATTATGAGG AGACCGATGG TGTGGTTTAT 
GTTAATGGGC TCCCGGAGGA TGAAGGGAGG TGTGAAGATG GAGGAGCGGA GGGAAGAACA 
CTGTTTTATG ATCATCTTGG TTTAGAGCAG CTCCAGGAAA CTTACATTCC CTCTGCCGGC 
ATTCGGACAC GGCCTACTGG ACCAGCACCA ACCAGCAGCG ACTTATGCTG GTACAAGAGC 
GCCCCCACTG CTCAGATGAC GGCTTTTGTC CAAGCGAAAG AGGAGTTGAA ACAACTAAAG 
CTGCCAGGCT TCCTGTACAG TGAGGTGGCA GATCTGGCAT CCACTGTGCC ATATTTCAGC 
ATGGATGAGG ATGAAAACTG TGATGATGGC ATACACCTTA TAGTGTGTGT ACACGGCTTG 
GATGGTAACA GTGCAGATTT GCGCCTGGTG AAGACTTATC TGGAGCTTGG GTTGCCTGGA 
GCTAGGATAG ATTTTCTGAT GTCTGAGCGA AATCAGAATG ACACATTTGC TGATTTTGAG 
TCCATGACCG ACAGACTTTT GGATGAAATT GTGCAGTACA TACAAATCTA CAACCTCACA 
GTTTCCAAGA TCAGTTTTGT TGGTCATTCT CTGGGAAATC TGATCGTGCG CTCTGTGCTG 
ACGAGGCCCA GGTTTAAGTG TTATCTCAGC CGACTGCACA CCTTTCTGTC TCTGTCTGGA 
CCTCACCTCG GCACACTGTA CAACAGCTCA GCACTCGTCA ATACTGGACT GTGGTTCATG 
CAGAAGTGGA AGAAGTCTGG CTCTCTGCTG CAGCTGACCT GTCGAGACCA TTCAGACCCG 
CGTCAGACCT TTCTCTATAA GCTGAGCAAG AAAGCAGGCC TTCAGTTCTT CAAGAATGTG 
GTGTTGGTGG GCTCTCTTCA GGACCGATAC GTTCCTTATC ACTCCGCTCG AATCGAGATG 
TGCAAAACAG CCTTAAAGGA CAAACAGACT GGTCCGGTGT ATGCTGAGAT GATCGAGAAC 
CTTCTCCTTC CTGTGCTTCA GAACAAAGAC TGTAACCTGG TGCGTTACGA CGTCATCCAC 
GCGCTGCCCA ACACCGCCAA CTCTCTGATC GGCCGCGCAG CTCACATCGC CGTCCTGGAC 
TCTGAGATTT TCCTGGAAAA GTTCTTCCTG GTGGCAGGGC TCAAGTTTTT TCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam135a ( XM_009307481.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305756.1 Danio rerio family with sequence similarity 135 member A (fam135a), transcript variant X4, mRNA.

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CloneID	ODa44872
Clone ID Related Accession (Same CDS sequence)	XM_009307481.2 , XM_009307481.3
Accession Version	XM_009307481.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein FAM135A isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307481.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGTA 
GAAGAGTCTT TAAATGAAGT TGACTTTCAG CTGTGTTTGG ACCTGTTCTT CACTGATGGA 
GACTACTCGC CTGAGGAGCC CAGTTCTCTG CAGAGCATCA GCAGCCGTAC GCTGCGTTTG 
CACTTTAGCC TCCACAGAGG CATCCACCAC CACGTCAACG TCATGTTTGA CTACTTCCAC 
CTGTCTGTCA TCTCTGCCAC AATCCATGCT TCACTGGTGG CTCTGCATCA GCCTCTAATA 
AGTTTCCCCC GGCCAGTAAA AAGCACCTGG TTGAATCGTA ATGCTCCGGC ACAGAGCAAA 
GACTCCCTCA TCCCACCGCT GGAGACTGTG GTGTTCGGCA GCACCTACGT CAAGCAGCTG 
TCCCCTGATG GCAAAAGCTT CCTGGTATCG GATCAGTGCC TCCAGCATGC CTTCAGCCTG 
CACCAGAACA TGTGTGCCAA CCTGCTGCGG GCATATCAGA GCCTGTTCTG TTACTTAACC 
TCCCTGACCA AGAATCTGCC CGCATCCCAG CGCATGGAGC TTGCCAAGCC CAGCATGCAG 
GTCCTTTATG AACGAGTTCT CAGAAGACCT TACCCTCCAG GCCAAAGGCA CGTCTACATA 
GAGGAACTTG ATGTGGAGGT CCGACTTCAT GAGCTTTGTG AACAAGTCAA GAAAGCAGAG 
AGCCCAGATG AGTTGGCTGA ATTGGTGAAC ATGAATTTGG CGCAACTATG TTCCCTACTC 
ATGGCTTTGT GGGGGAATTT CCTGGAAGTT GTGTCACTAA ATGAGCGTGT TTTTGTGCTT 
TTGGCCCAGG AGCACCACAC ACTACGGGTG CAAAGATTTG CTGAAGCTTT CTTTTGTCTT 
GAACACCCAC GACAGAGTGC ACTGGCCTAC CAGGAACTGC ATGCTCACAG TCACCAACAA 
ATGACCACAG CTATCAAAAG CTCTTCCTAC TTCTTGTCCT TACCACCTCT TCCTGTTGAA 
TGTGCTGATC TTGACGGGGA TGTGAACTCC CTACCAATCA TCTTTGAGGA CAGATATTTG 
GAGTCTGTTA CTGAAGATCG TGATGGTCCA TGGCTTGGAC TCCAAAACAA CAGGAGTGGC 
TCTGTGTCCG CCAAAGCAGA TAGATCCAAC TCAAAGGACT GCATTGCCAG TGGAGCCCTC 
GGTCCGTATC CAGATAAGCA GCAAGCTTCC TTTGCCACTG CTTGGCCTGA ACAAAACGAG 
CCTTCCCTTA AATCCAAAGC CAAGTCCATC AAGCTCAAGA AGAACACCAA GACCGAAAAC 
TCAAAGAAGC TGATCAGACA GAGCTCGAAA GATTCTGTGG TGCTCACAGG CTACAAGAAC 
TTGAAGGTTC CTCACAGTGA AAAGACCATT AAGTCCAAAG ACATCGACCA TCAATGCAAT 
CAGAGAGATG AGCTAGAGGA TGGAGGTAAA TTCCTTCTTC AGAATGAAAC CACGACCACG 
CATTGTGCTC AGAATGCTAA AGACTGCACA TCTGGCAACC TTCAAGGTAA CGCCACAATC 
CTAAGTGACG GCTTTCCCAA TCTTATGAGT TTGCAAATGC AGGCCAGGCC CCAGGCAGGT 
ACTGGAGCAG AGGCCGCCGC TGCCAGAGTA GATCCCACAC ATCCACCTGC TGGCCTCAGA 
CATATTGATG TCAAACCCAG TGACAAGGAC CCCTACCAGG GCGATAAGGT CACCGTTCTC 
TTACCGCACC ACCCAGAGCG CACCACCAAG AGGGATGTTC CAGAAATCAC ACAGACTGAT 
TTCTCTGAGT TCAAGAGTCG AGTTGGAGAT GGTGCTACTT TCGTCGGACC TTGTGGAGCG 
GCTTTGAGGA ACGATAATAT TCCTCAAGCC TCCTCCAGAC TCTCGGATTC CGGAATTGAA 
AGCGAACCCA GCTCTGCGAC ACAGCTAGCC CCGGTTTCTC CATCTCCTTT TGCTCCTATT 
GGGATCGAAG CACCTACAGA GCTGATTCCT CACAGACCAC CTCCGCTCAG TCCTGCTTCC 
AGAGGAGCCA CCGCATTGGA AGGCCTCAAT CCTGAAAGCA CCAGCGGCGT AAGCGGCGTC 
CAATCTTCCC TCACCTCCAT CAACTCACTC CCTTCGGATG ATGAGGTGGA GACAGAGGCC 
ACCAGCAGGA AGTCCAGCAT CCTGGTCCAG GAACAGAGTC TCATCTTCTC CGGAGAATCT 
CAAAGCCTCT TGCTGACAAA CTCTAGAGTC TTGGCTCAAC AAAAAGCCAG TCATGGAGAT 
GGGATGGACT CTGCTTTGCA GTCCCGCTGT GAAACTTCTG CTTCTGACAG ACTTGTCGGA 
TCTAACGAGA CAGATCTTCC AGGTGGTCCG GAAGCTTGTA GTGAACCACA GACAGCAGCC 
CAGTCCAGTA CATCAGTCAC CAGCAGCACA GACATGGTGA AGAAAGGCTT TGTAGAAAAC 
TACTTTGGCT CTCGGTCCAG TACTGATATA TCTGAAATCA GCCCTTTAGA GACGGCAGCC 
ATAACATTGG GTATTCAGAC ATGCCCCAAT GCTAATGATG AAGAGGATGA GGAGGAAGAG 
CAGGAGCATG AGATGATTGA GAATGGTTAT TATGAGGAGA CCGATGGTGT GGTTTATGTT 
AATGGGCTCC CGGAGGATGA AGGGAGGTGT GAAGATGGAG GAGCGGAGGG AAGAACACTG 
TTTTATGATC ATCTTGGTTT AGAGCAGCTC CAGGAAACTT ACATTCCCTC TGCCGGCATT 
CGGACACGGC CTACTGGACC AGCACCAACC AGCAGCGACT TATGCTGGTA CAAGAGCGCC 
CCCACTGCTC AGATGACGGC TTTTGTCCAA GCGAAAGAGG AGTTGAAACA ACTAAAGCTG 
CCAGGCTTCC TGTACAGTGA GGTGGCAGAT CTGGCATCCA CTGTGCCATA TTTCAGCATG 
GATGAGGATG AAAACTGTGA TGATGGCATA CACCTTATAG TGTGTGTACA CGGCTTGGAT 
GGTAACAGTG CAGATTTGCG CCTGGTGAAG ACTTATCTGG AGCTTGGGTT GCCTGGAGCT 
AGGATAGATT TTCTGATGTC TGAGCGAAAT CAGAATGACA CATTTGCTGA TTTTGAGTCC 
ATGACCGACA GACTTTTGGA TGAAATTGTG CAGTACATAC AAATCTACAA CCTCACAGTT 
TCCAAGATCA GTTTTGTTGG TCATTCTCTG GGAAATCTGA TCGTGCGCTC TGTGCTGACG 
AGGCCCAGGT TTAAGTGTTA TCTCAGCCGA CTGCACACCT TTCTGTCTCT GTCTGGACCT 
CACCTCGGCA CACTGTACAA CAGCTCAGCA CTCGTCAATA CTGGACTGTG GTTCATGCAG 
AAGTGGAAGA AGTCTGGCTC TCTGCTGCAG CTGACCTGTC GAGACCATTC AGACCCGCGT 
CAGACCTTTC TCTATAAGCT GAGCAAGAAA GCAGGCCTTC AGTTCTTCAA GAATGTGGTG 
TTGGTGGGCT CTCTTCAGGA CCGATACGTT CCTTATCACT CCGCTCGAAT CGAGATGTGC 
AAAACAGCCT TAAAGGACAA ACAGACTGGT CCGGTGTATG CTGAGATGAT CGAGAACCTT 
CTCCTTCCTG TGCTTCAGAA CAAAGACTGT AACCTGGTGC GTTACGACGT CATCCACGCG 
CTGCCCAACA CCGCCAACTC TCTGATCGGC CGCGCAGCTC ACATCGCCGT CCTGGACTCT 
GAGATTTTCC TGGAAAAGTT CTTCCTGGTG GCAGGGCTCA AGTTTTTTCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305756.1
CDS	388..4401
Translation	MTEVQATVEFSVELHKFYNVDLFQRGFYQVRASMKVPPRIPQRVEASLLHPIGSDLAFPASVQDDVVCSKTFQILYKNEEIVVNDVFVFKVMMLLDAKKVEESLNEVDFQLCLDLFFTDGDYSPEEPSSLQSISSRTLRLHFSLHRGIHHHVNVMFDYFHLSVISATIHASLVALHQPLISFPRPVKSTWLNRNAPAQSKDSLIPPLETVVFGSTYVKQLSPDGKSFLVSDQCLQHAFSLHQNMCANLLRAYQSLFCYLTSLTKNLPASQRMELAKPSMQVLYERVLRRPYPPGQRHVYIEELDVEVRLHELCEQVKKAESPDELAELVNMNLAQLCSLLMALWGNFLEVVSLNERVFVLLAQEHHTLRVQRFAEAFFCLEHPRQSALAYQELHAHSHQQMTTAIKSSSYFLSLPPLPVECADLDGDVNSLPIIFEDRYLESVTEDRDGPWLGLQNNRSGSVSAKADRSNSKDCIASGALGPYPDKQQASFATAWPEQNEPSLKSKAKSIKLKKNTKTENSKKLIRQSSKDSVVLTGYKNLKVPHSEKTIKSKDIDHQCNQRDELEDGGKFLLQNETTTTHCAQNAKDCTSGNLQGNATILSDGFPNLMSLQMQARPQAGTGAEAAAARVDPTHPPAGLRHIDVKPSDKDPYQGDKVTVLLPHHPERTTKRDVPEITQTDFSEFKSRVGDGATFVGPCGAALRNDNIPQASSRLSDSGIESEPSSATQLAPVSPSPFAPIGIEAPTELIPHRPPPLSPASRGATALEGLNPESTSGVSGVQSSLTSINSLPSDDEVETEATSRKSSILVQEQSLIFSGESQSLLLTNSRVLAQQKASHGDGMDSALQSRCETSASDRLVGSNETDLPGGPEACSEPQTAAQSSTSVTSSTDMVKKGFVENYFGSRSSTDISEISPLETAAITLGIQTCPNANDEEDEEEEQEHEMIENGYYEETDGVVYVNGLPEDEGRCEDGGAEGRTLFYDHLGLEQLQETYIPSAGIRTRPTGPAPTSSDLCWYKSAPTAQMTAFVQAKEELKQLKLPGFLYSEVADLASTVPYFSMDEDENCDDGIHLIVCVHGLDGNSADLRLVKTYLELGLPGARIDFLMSERNQNDTFADFESMTDRLLDEIVQYIQIYNLTVSKISFVGHSLGNLIVRSVLTRPRFKCYLSRLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSKKAGLQFFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQTGPVYAEMIENLLLPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEIFLEKFFLVAGLKFFQ

Target ORF information:

RefSeq Version	XM_009307481.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio family with sequence similarity 135 member A (fam135a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307481.2

ORF Insert Sequence:

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGTA 
GAAGAGTCTT TAAATGAAGT TGACTTTCAG CTGTGTTTGG ACCTGTTCTT CACTGATGGA 
GACTACTCGC CTGAGGAGCC CAGTTCTCTG CAGAGCATCA GCAGCCGTAC GCTGCGTTTG 
CACTTTAGCC TCCACAGAGG CATCCACCAC CACGTCAACG TCATGTTTGA CTACTTCCAC 
CTGTCTGTCA TCTCTGCCAC AATCCATGCT TCACTGGTGG CTCTGCATCA GCCTCTAATA 
AGTTTCCCCC GGCCAGTAAA AAGCACCTGG TTGAATCGTA ATGCTCCGGC ACAGAGCAAA 
GACTCCCTCA TCCCACCGCT GGAGACTGTG GTGTTCGGCA GCACCTACGT CAAGCAGCTG 
TCCCCTGATG GCAAAAGCTT CCTGGTATCG GATCAGTGCC TCCAGCATGC CTTCAGCCTG 
CACCAGAACA TGTGTGCCAA CCTGCTGCGG GCATATCAGA GCCTGTTCTG TTACTTAACC 
TCCCTGACCA AGAATCTGCC CGCATCCCAG CGCATGGAGC TTGCCAAGCC CAGCATGCAG 
GTCCTTTATG AACGAGTTCT CAGAAGACCT TACCCTCCAG GCCAAAGGCA CGTCTACATA 
GAGGAACTTG ATGTGGAGGT CCGACTTCAT GAGCTTTGTG AACAAGTCAA GAAAGCAGAG 
AGCCCAGATG AGTTGGCTGA ATTGGTGAAC ATGAATTTGG CGCAACTATG TTCCCTACTC 
ATGGCTTTGT GGGGGAATTT CCTGGAAGTT GTGTCACTAA ATGAGCGTGT TTTTGTGCTT 
TTGGCCCAGG AGCACCACAC ACTACGGGTG CAAAGATTTG CTGAAGCTTT CTTTTGTCTT 
GAACACCCAC GACAGAGTGC ACTGGCCTAC CAGGAACTGC ATGCTCACAG TCACCAACAA 
ATGACCACAG CTATCAAAAG CTCTTCCTAC TTCTTGTCCT TACCACCTCT TCCTGTTGAA 
TGTGCTGATC TTGACGGGGA TGTGAACTCC CTACCAATCA TCTTTGAGGA CAGATATTTG 
GAGTCTGTTA CTGAAGATCG TGATGGTCCA TGGCTTGGAC TCCAAAACAA CAGGAGTGGC 
TCTGTGTCCG CCAAAGCAGA TAGATCCAAC TCAAAGGACT GCATTGCCAG TGGAGCCCTC 
GGTCCGTATC CAGATAAGCA GCAAGCTTCC TTTGCCACTG CTTGGCCTGA ACAAAACGAG 
CCTTCCCTTA AATCCAAAGC CAAGTCCATC AAGCTCAAGA AGAACACCAA GACCGAAAAC 
TCAAAGAAGC TGATCAGACA GAGCTCGAAA GATTCTGTGG TGCTCACAGG CTACAAGAAC 
TTGAAGGTTC CTCACAGTGA AAAGACCATT AAGTCCAAAG ACATCGACCA TCAATGCAAT 
CAGAGAGATG AGCTAGAGGA TGGAGGTAAA TTCCTTCTTC AGAATGAAAC CACGACCACG 
CATTGTGCTC AGAATGCTAA AGACTGCACA TCTGGCAACC TTCAAGGTAA CGCCACAATC 
CTAAGTGACG GCTTTCCCAA TCTTATGAGT TTGCAAATGC AGGCCAGGCC CCAGGCAGGT 
ACTGGAGCAG AGGCCGCCGC TGCCAGAGTA GATCCCACAC ATCCACCTGC TGGCCTCAGA 
CATATTGATG TCAAACCCAG TGACAAGGAC CCCTACCAGG GCGATAAGGT CACCGTTCTC 
TTACCGCACC ACCCAGAGCG CACCACCAAG AGGGATGTTC CAGAAATCAC ACAGACTGAT 
TTCTCTGAGT TCAAGAGTCG AGTTGGAGAT GGTGCTACTT TCGTCGGACC TTGTGGAGCG 
GCTTTGAGGA ACGATAATAT TCCTCAAGCC TCCTCCAGAC TCTCGGATTC CGGAATTGAA 
AGCGAACCCA GCTCTGCGAC ACAGCTAGCC CCGGTTTCTC CATCTCCTTT TGCTCCTATT 
GGGATCGAAG CACCTACAGA GCTGATTCCT CACAGACCAC CTCCGCTCAG TCCTGCTTCC 
AGAGGAGCCA CCGCATTGGA AGGCCTCAAT CCTGAAAGCA CCAGCGGCGT AAGCGGCGTC 
CAATCTTCCC TCACCTCCAT CAACTCACTC CCTTCGGATG ATGAGGTGGA GACAGAGGCC 
ACCAGCAGGA AGTCCAGCAT CCTGGTCCAG GAACAGAGTC TCATCTTCTC CGGAGAATCT 
CAAAGCCTCT TGCTGACAAA CTCTAGAGTC TTGGCTCAAC AAAAAGCCAG TCATGGAGAT 
GGGATGGACT CTGCTTTGCA GTCCCGCTGT GAAACTTCTG CTTCTGACAG ACTTGTCGGA 
TCTAACGAGA CAGATCTTCC AGGTGGTCCG GAAGCTTGTA GTGAACCACA GACAGCAGCC 
CAGTCCAGTA CATCAGTCAC CAGCAGCACA GACATGGTGA AGAAAGGCTT TGTAGAAAAC 
TACTTTGGCT CTCGGTCCAG TACTGATATA TCTGAAATCA GCCCTTTAGA GACGGCAGCC 
ATAACATTGG GTATTCAGAC ATGCCCCAAT GCTAATGATG AAGAGGATGA GGAGGAAGAG 
CAGGAGCATG AGATGATTGA GAATGGTTAT TATGAGGAGA CCGATGGTGT GGTTTATGTT 
AATGGGCTCC CGGAGGATGA AGGGAGGTGT GAAGATGGAG GAGCGGAGGG AAGAACACTG 
TTTTATGATC ATCTTGGTTT AGAGCAGCTC CAGGAAACTT ACATTCCCTC TGCCGGCATT 
CGGACACGGC CTACTGGACC AGCACCAACC AGCAGCGACT TATGCTGGTA CAAGAGCGCC 
CCCACTGCTC AGATGACGGC TTTTGTCCAA GCGAAAGAGG AGTTGAAACA ACTAAAGCTG 
CCAGGCTTCC TGTACAGTGA GGTGGCAGAT CTGGCATCCA CTGTGCCATA TTTCAGCATG 
GATGAGGATG AAAACTGTGA TGATGGCATA CACCTTATAG TGTGTGTACA CGGCTTGGAT 
GGTAACAGTG CAGATTTGCG CCTGGTGAAG ACTTATCTGG AGCTTGGGTT GCCTGGAGCT 
AGGATAGATT TTCTGATGTC TGAGCGAAAT CAGAATGACA CATTTGCTGA TTTTGAGTCC 
ATGACCGACA GACTTTTGGA TGAAATTGTG CAGTACATAC AAATCTACAA CCTCACAGTT 
TCCAAGATCA GTTTTGTTGG TCATTCTCTG GGAAATCTGA TCGTGCGCTC TGTGCTGACG 
AGGCCCAGGT TTAAGTGTTA TCTCAGCCGA CTGCACACCT TTCTGTCTCT GTCTGGACCT 
CACCTCGGCA CACTGTACAA CAGCTCAGCA CTCGTCAATA CTGGACTGTG GTTCATGCAG 
AAGTGGAAGA AGTCTGGCTC TCTGCTGCAG CTGACCTGTC GAGACCATTC AGACCCGCGT 
CAGACCTTTC TCTATAAGCT GAGCAAGAAA GCAGGCCTTC AGTTCTTCAA GAATGTGGTG 
TTGGTGGGCT CTCTTCAGGA CCGATACGTT CCTTATCACT CCGCTCGAAT CGAGATGTGC 
AAAACAGCCT TAAAGGACAA ACAGACTGGT CCGGTGTATG CTGAGATGAT CGAGAACCTT 
CTCCTTCCTG TGCTTCAGAA CAAAGACTGT AACCTGGTGC GTTACGACGT CATCCACGCG 
CTGCCCAACA CCGCCAACTC TCTGATCGGC CGCGCAGCTC ACATCGCCGT CCTGGACTCT 
GAGATTTTCC TGGAAAAGTT CTTCCTGGTG GCAGGGCTCA AGTTTTTTCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

fam135a ( XM_009307481.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305756.1 Danio rerio family with sequence similarity 135, member A (fam135a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa44872
Clone ID Related Accession (Same CDS sequence)	XM_009307481.2 , XM_009307481.3
Accession Version	XM_009307481.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein FAM135A isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307481.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGTA 
GAAGAGTCTT TAAATGAAGT TGACTTTCAG CTGTGTTTGG ACCTGTTCTT CACTGATGGA 
GACTACTCGC CTGAGGAGCC CAGTTCTCTG CAGAGCATCA GCAGCCGTAC GCTGCGTTTG 
CACTTTAGCC TCCACAGAGG CATCCACCAC CACGTCAACG TCATGTTTGA CTACTTCCAC 
CTGTCTGTCA TCTCTGCCAC AATCCATGCT TCACTGGTGG CTCTGCATCA GCCTCTAATA 
AGTTTCCCCC GGCCAGTAAA AAGCACCTGG TTGAATCGTA ATGCTCCGGC ACAGAGCAAA 
GACTCCCTCA TCCCACCGCT GGAGACTGTG GTGTTCGGCA GCACCTACGT CAAGCAGCTG 
TCCCCTGATG GCAAAAGCTT CCTGGTATCG GATCAGTGCC TCCAGCATGC CTTCAGCCTG 
CACCAGAACA TGTGTGCCAA CCTGCTGCGG GCATATCAGA GCCTGTTCTG TTACTTAACC 
TCCCTGACCA AGAATCTGCC CGCATCCCAG CGCATGGAGC TTGCCAAGCC CAGCATGCAG 
GTCCTTTATG AACGAGTTCT CAGAAGACCT TACCCTCCAG GCCAAAGGCA CGTCTACATA 
GAGGAACTTG ATGTGGAGGT CCGACTTCAT GAGCTTTGTG AACAAGTCAA GAAAGCAGAG 
AGCCCAGATG AGTTGGCTGA ATTGGTGAAC ATGAATTTGG CGCAACTATG TTCCCTACTC 
ATGGCTTTGT GGGGGAATTT CCTGGAAGTT GTGTCACTAA ATGAGCGTGT TTTTGTGCTT 
TTGGCCCAGG AGCACCACAC ACTACGGGTG CAAAGATTTG CTGAAGCTTT CTTTTGTCTT 
GAACACCCAC GACAGAGTGC ACTGGCCTAC CAGGAACTGC ATGCTCACAG TCACCAACAA 
ATGACCACAG CTATCAAAAG CTCTTCCTAC TTCTTGTCCT TACCACCTCT TCCTGTTGAA 
TGTGCTGATC TTGACGGGGA TGTGAACTCC CTACCAATCA TCTTTGAGGA CAGATATTTG 
GAGTCTGTTA CTGAAGATCG TGATGGTCCA TGGCTTGGAC TCCAAAACAA CAGGAGTGGC 
TCTGTGTCCG CCAAAGCAGA TAGATCCAAC TCAAAGGACT GCATTGCCAG TGGAGCCCTC 
GGTCCGTATC CAGATAAGCA GCAAGCTTCC TTTGCCACTG CTTGGCCTGA ACAAAACGAG 
CCTTCCCTTA AATCCAAAGC CAAGTCCATC AAGCTCAAGA AGAACACCAA GACCGAAAAC 
TCAAAGAAGC TGATCAGACA GAGCTCGAAA GATTCTGTGG TGCTCACAGG CTACAAGAAC 
TTGAAGGTTC CTCACAGTGA AAAGACCATT AAGTCCAAAG ACATCGACCA TCAATGCAAT 
CAGAGAGATG AGCTAGAGGA TGGAGGTAAA TTCCTTCTTC AGAATGAAAC CACGACCACG 
CATTGTGCTC AGAATGCTAA AGACTGCACA TCTGGCAACC TTCAAGGTAA CGCCACAATC 
CTAAGTGACG GCTTTCCCAA TCTTATGAGT TTGCAAATGC AGGCCAGGCC CCAGGCAGGT 
ACTGGAGCAG AGGCCGCCGC TGCCAGAGTA GATCCCACAC ATCCACCTGC TGGCCTCAGA 
CATATTGATG TCAAACCCAG TGACAAGGAC CCCTACCAGG GCGATAAGGT CACCGTTCTC 
TTACCGCACC ACCCAGAGCG CACCACCAAG AGGGATGTTC CAGAAATCAC ACAGACTGAT 
TTCTCTGAGT TCAAGAGTCG AGTTGGAGAT GGTGCTACTT TCGTCGGACC TTGTGGAGCG 
GCTTTGAGGA ACGATAATAT TCCTCAAGCC TCCTCCAGAC TCTCGGATTC CGGAATTGAA 
AGCGAACCCA GCTCTGCGAC ACAGCTAGCC CCGGTTTCTC CATCTCCTTT TGCTCCTATT 
GGGATCGAAG CACCTACAGA GCTGATTCCT CACAGACCAC CTCCGCTCAG TCCTGCTTCC 
AGAGGAGCCA CCGCATTGGA AGGCCTCAAT CCTGAAAGCA CCAGCGGCGT AAGCGGCGTC 
CAATCTTCCC TCACCTCCAT CAACTCACTC CCTTCGGATG ATGAGGTGGA GACAGAGGCC 
ACCAGCAGGA AGTCCAGCAT CCTGGTCCAG GAACAGAGTC TCATCTTCTC CGGAGAATCT 
CAAAGCCTCT TGCTGACAAA CTCTAGAGTC TTGGCTCAAC AAAAAGCCAG TCATGGAGAT 
GGGATGGACT CTGCTTTGCA GTCCCGCTGT GAAACTTCTG CTTCTGACAG ACTTGTCGGA 
TCTAACGAGA CAGATCTTCC AGGTGGTCCG GAAGCTTGTA GTGAACCACA GACAGCAGCC 
CAGTCCAGTA CATCAGTCAC CAGCAGCACA GACATGGTGA AGAAAGGCTT TGTAGAAAAC 
TACTTTGGCT CTCGGTCCAG TACTGATATA TCTGAAATCA GCCCTTTAGA GACGGCAGCC 
ATAACATTGG GTATTCAGAC ATGCCCCAAT GCTAATGATG AAGAGGATGA GGAGGAAGAG 
CAGGAGCATG AGATGATTGA GAATGGTTAT TATGAGGAGA CCGATGGTGT GGTTTATGTT 
AATGGGCTCC CGGAGGATGA AGGGAGGTGT GAAGATGGAG GAGCGGAGGG AAGAACACTG 
TTTTATGATC ATCTTGGTTT AGAGCAGCTC CAGGAAACTT ACATTCCCTC TGCCGGCATT 
CGGACACGGC CTACTGGACC AGCACCAACC AGCAGCGACT TATGCTGGTA CAAGAGCGCC 
CCCACTGCTC AGATGACGGC TTTTGTCCAA GCGAAAGAGG AGTTGAAACA ACTAAAGCTG 
CCAGGCTTCC TGTACAGTGA GGTGGCAGAT CTGGCATCCA CTGTGCCATA TTTCAGCATG 
GATGAGGATG AAAACTGTGA TGATGGCATA CACCTTATAG TGTGTGTACA CGGCTTGGAT 
GGTAACAGTG CAGATTTGCG CCTGGTGAAG ACTTATCTGG AGCTTGGGTT GCCTGGAGCT 
AGGATAGATT TTCTGATGTC TGAGCGAAAT CAGAATGACA CATTTGCTGA TTTTGAGTCC 
ATGACCGACA GACTTTTGGA TGAAATTGTG CAGTACATAC AAATCTACAA CCTCACAGTT 
TCCAAGATCA GTTTTGTTGG TCATTCTCTG GGAAATCTGA TCGTGCGCTC TGTGCTGACG 
AGGCCCAGGT TTAAGTGTTA TCTCAGCCGA CTGCACACCT TTCTGTCTCT GTCTGGACCT 
CACCTCGGCA CACTGTACAA CAGCTCAGCA CTCGTCAATA CTGGACTGTG GTTCATGCAG 
AAGTGGAAGA AGTCTGGCTC TCTGCTGCAG CTGACCTGTC GAGACCATTC AGACCCGCGT 
CAGACCTTTC TCTATAAGCT GAGCAAGAAA GCAGGCCTTC AGTTCTTCAA GAATGTGGTG 
TTGGTGGGCT CTCTTCAGGA CCGATACGTT CCTTATCACT CCGCTCGAAT CGAGATGTGC 
AAAACAGCCT TAAAGGACAA ACAGACTGGT CCGGTGTATG CTGAGATGAT CGAGAACCTT 
CTCCTTCCTG TGCTTCAGAA CAAAGACTGT AACCTGGTGC GTTACGACGT CATCCACGCG 
CTGCCCAACA CCGCCAACTC TCTGATCGGC CGCGCAGCTC ACATCGCCGT CCTGGACTCT 
GAGATTTTCC TGGAAAAGTT CTTCCTGGTG GCAGGGCTCA AGTTTTTTCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305756.1
CDS	405..4418
Translation	MTEVQATVEFSVELHKFYNVDLFQRGFYQVRASMKVPPRIPQRVEASLLHPIGSDLAFPASVQDDVVCSKTFQILYKNEEIVVNDVFVFKVMMLLDAKKVEESLNEVDFQLCLDLFFTDGDYSPEEPSSLQSISSRTLRLHFSLHRGIHHHVNVMFDYFHLSVISATIHASLVALHQPLISFPRPVKSTWLNRNAPAQSKDSLIPPLETVVFGSTYVKQLSPDGKSFLVSDQCLQHAFSLHQNMCANLLRAYQSLFCYLTSLTKNLPASQRMELAKPSMQVLYERVLRRPYPPGQRHVYIEELDVEVRLHELCEQVKKAESPDELAELVNMNLAQLCSLLMALWGNFLEVVSLNERVFVLLAQEHHTLRVQRFAEAFFCLEHPRQSALAYQELHAHSHQQMTTAIKSSSYFLSLPPLPVECADLDGDVNSLPIIFEDRYLESVTEDRDGPWLGLQNNRSGSVSAKADRSNSKDCIASGALGPYPDKQQASFATAWPEQNEPSLKSKAKSIKLKKNTKTENSKKLIRQSSKDSVVLTGYKNLKVPHSEKTIKSKDIDHQCNQRDELEDGGKFLLQNETTTTHCAQNAKDCTSGNLQGNATILSDGFPNLMSLQMQARPQAGTGAEAAAARVDPTHPPAGLRHIDVKPSDKDPYQGDKVTVLLPHHPERTTKRDVPEITQTDFSEFKSRVGDGATFVGPCGAALRNDNIPQASSRLSDSGIESEPSSATQLAPVSPSPFAPIGIEAPTELIPHRPPPLSPASRGATALEGLNPESTSGVSGVQSSLTSINSLPSDDEVETEATSRKSSILVQEQSLIFSGESQSLLLTNSRVLAQQKASHGDGMDSALQSRCETSASDRLVGSNETDLPGGPEACSEPQTAAQSSTSVTSSTDMVKKGFVENYFGSRSSTDISEISPLETAAITLGIQTCPNANDEEDEEEEQEHEMIENGYYEETDGVVYVNGLPEDEGRCEDGGAEGRTLFYDHLGLEQLQETYIPSAGIRTRPTGPAPTSSDLCWYKSAPTAQMTAFVQAKEELKQLKLPGFLYSEVADLASTVPYFSMDEDENCDDGIHLIVCVHGLDGNSADLRLVKTYLELGLPGARIDFLMSERNQNDTFADFESMTDRLLDEIVQYIQIYNLTVSKISFVGHSLGNLIVRSVLTRPRFKCYLSRLHTFLSLSGPHLGTLYNSSALVNTGLWFMQKWKKSGSLLQLTCRDHSDPRQTFLYKLSKKAGLQFFKNVVLVGSLQDRYVPYHSARIEMCKTALKDKQTGPVYAEMIENLLLPVLQNKDCNLVRYDVIHALPNTANSLIGRAAHIAVLDSEIFLEKFFLVAGLKFFQ

Target ORF information:

RefSeq Version	XM_009307481.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio family with sequence similarity 135, member A (fam135a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307481.3

ORF Insert Sequence:

ATGACGGAGG TCCAGGCCAC AGTGGAGTTC TCTGTGGAGC TCCACAAATT CTACAATGTG 
GATCTCTTCC AAAGAGGATT CTATCAGGTC CGAGCCAGTA TGAAAGTGCC GCCACGGATC 
CCGCAGAGGG TGGAAGCCAG TCTGCTGCAT CCCATAGGTT CGGATCTGGC TTTTCCAGCA 
TCAGTTCAAG ATGATGTGGT CTGCAGCAAA ACCTTTCAGA TCCTTTACAA AAACGAGGAG 
ATTGTGGTCA ACGACGTCTT TGTATTCAAA GTCATGATGC TGCTCGATGC AAAGAAGGTA 
GAAGAGTCTT TAAATGAAGT TGACTTTCAG CTGTGTTTGG ACCTGTTCTT CACTGATGGA 
GACTACTCGC CTGAGGAGCC CAGTTCTCTG CAGAGCATCA GCAGCCGTAC GCTGCGTTTG 
CACTTTAGCC TCCACAGAGG CATCCACCAC CACGTCAACG TCATGTTTGA CTACTTCCAC 
CTGTCTGTCA TCTCTGCCAC AATCCATGCT TCACTGGTGG CTCTGCATCA GCCTCTAATA 
AGTTTCCCCC GGCCAGTAAA AAGCACCTGG TTGAATCGTA ATGCTCCGGC ACAGAGCAAA 
GACTCCCTCA TCCCACCGCT GGAGACTGTG GTGTTCGGCA GCACCTACGT CAAGCAGCTG 
TCCCCTGATG GCAAAAGCTT CCTGGTATCG GATCAGTGCC TCCAGCATGC CTTCAGCCTG 
CACCAGAACA TGTGTGCCAA CCTGCTGCGG GCATATCAGA GCCTGTTCTG TTACTTAACC 
TCCCTGACCA AGAATCTGCC CGCATCCCAG CGCATGGAGC TTGCCAAGCC CAGCATGCAG 
GTCCTTTATG AACGAGTTCT CAGAAGACCT TACCCTCCAG GCCAAAGGCA CGTCTACATA 
GAGGAACTTG ATGTGGAGGT CCGACTTCAT GAGCTTTGTG AACAAGTCAA GAAAGCAGAG 
AGCCCAGATG AGTTGGCTGA ATTGGTGAAC ATGAATTTGG CGCAACTATG TTCCCTACTC 
ATGGCTTTGT GGGGGAATTT CCTGGAAGTT GTGTCACTAA ATGAGCGTGT TTTTGTGCTT 
TTGGCCCAGG AGCACCACAC ACTACGGGTG CAAAGATTTG CTGAAGCTTT CTTTTGTCTT 
GAACACCCAC GACAGAGTGC ACTGGCCTAC CAGGAACTGC ATGCTCACAG TCACCAACAA 
ATGACCACAG CTATCAAAAG CTCTTCCTAC TTCTTGTCCT TACCACCTCT TCCTGTTGAA 
TGTGCTGATC TTGACGGGGA TGTGAACTCC CTACCAATCA TCTTTGAGGA CAGATATTTG 
GAGTCTGTTA CTGAAGATCG TGATGGTCCA TGGCTTGGAC TCCAAAACAA CAGGAGTGGC 
TCTGTGTCCG CCAAAGCAGA TAGATCCAAC TCAAAGGACT GCATTGCCAG TGGAGCCCTC 
GGTCCGTATC CAGATAAGCA GCAAGCTTCC TTTGCCACTG CTTGGCCTGA ACAAAACGAG 
CCTTCCCTTA AATCCAAAGC CAAGTCCATC AAGCTCAAGA AGAACACCAA GACCGAAAAC 
TCAAAGAAGC TGATCAGACA GAGCTCGAAA GATTCTGTGG TGCTCACAGG CTACAAGAAC 
TTGAAGGTTC CTCACAGTGA AAAGACCATT AAGTCCAAAG ACATCGACCA TCAATGCAAT 
CAGAGAGATG AGCTAGAGGA TGGAGGTAAA TTCCTTCTTC AGAATGAAAC CACGACCACG 
CATTGTGCTC AGAATGCTAA AGACTGCACA TCTGGCAACC TTCAAGGTAA CGCCACAATC 
CTAAGTGACG GCTTTCCCAA TCTTATGAGT TTGCAAATGC AGGCCAGGCC CCAGGCAGGT 
ACTGGAGCAG AGGCCGCCGC TGCCAGAGTA GATCCCACAC ATCCACCTGC TGGCCTCAGA 
CATATTGATG TCAAACCCAG TGACAAGGAC CCCTACCAGG GCGATAAGGT CACCGTTCTC 
TTACCGCACC ACCCAGAGCG CACCACCAAG AGGGATGTTC CAGAAATCAC ACAGACTGAT 
TTCTCTGAGT TCAAGAGTCG AGTTGGAGAT GGTGCTACTT TCGTCGGACC TTGTGGAGCG 
GCTTTGAGGA ACGATAATAT TCCTCAAGCC TCCTCCAGAC TCTCGGATTC CGGAATTGAA 
AGCGAACCCA GCTCTGCGAC ACAGCTAGCC CCGGTTTCTC CATCTCCTTT TGCTCCTATT 
GGGATCGAAG CACCTACAGA GCTGATTCCT CACAGACCAC CTCCGCTCAG TCCTGCTTCC 
AGAGGAGCCA CCGCATTGGA AGGCCTCAAT CCTGAAAGCA CCAGCGGCGT AAGCGGCGTC 
CAATCTTCCC TCACCTCCAT CAACTCACTC CCTTCGGATG ATGAGGTGGA GACAGAGGCC 
ACCAGCAGGA AGTCCAGCAT CCTGGTCCAG GAACAGAGTC TCATCTTCTC CGGAGAATCT 
CAAAGCCTCT TGCTGACAAA CTCTAGAGTC TTGGCTCAAC AAAAAGCCAG TCATGGAGAT 
GGGATGGACT CTGCTTTGCA GTCCCGCTGT GAAACTTCTG CTTCTGACAG ACTTGTCGGA 
TCTAACGAGA CAGATCTTCC AGGTGGTCCG GAAGCTTGTA GTGAACCACA GACAGCAGCC 
CAGTCCAGTA CATCAGTCAC CAGCAGCACA GACATGGTGA AGAAAGGCTT TGTAGAAAAC 
TACTTTGGCT CTCGGTCCAG TACTGATATA TCTGAAATCA GCCCTTTAGA GACGGCAGCC 
ATAACATTGG GTATTCAGAC ATGCCCCAAT GCTAATGATG AAGAGGATGA GGAGGAAGAG 
CAGGAGCATG AGATGATTGA GAATGGTTAT TATGAGGAGA CCGATGGTGT GGTTTATGTT 
AATGGGCTCC CGGAGGATGA AGGGAGGTGT GAAGATGGAG GAGCGGAGGG AAGAACACTG 
TTTTATGATC ATCTTGGTTT AGAGCAGCTC CAGGAAACTT ACATTCCCTC TGCCGGCATT 
CGGACACGGC CTACTGGACC AGCACCAACC AGCAGCGACT TATGCTGGTA CAAGAGCGCC 
CCCACTGCTC AGATGACGGC TTTTGTCCAA GCGAAAGAGG AGTTGAAACA ACTAAAGCTG 
CCAGGCTTCC TGTACAGTGA GGTGGCAGAT CTGGCATCCA CTGTGCCATA TTTCAGCATG 
GATGAGGATG AAAACTGTGA TGATGGCATA CACCTTATAG TGTGTGTACA CGGCTTGGAT 
GGTAACAGTG CAGATTTGCG CCTGGTGAAG ACTTATCTGG AGCTTGGGTT GCCTGGAGCT 
AGGATAGATT TTCTGATGTC TGAGCGAAAT CAGAATGACA CATTTGCTGA TTTTGAGTCC 
ATGACCGACA GACTTTTGGA TGAAATTGTG CAGTACATAC AAATCTACAA CCTCACAGTT 
TCCAAGATCA GTTTTGTTGG TCATTCTCTG GGAAATCTGA TCGTGCGCTC TGTGCTGACG 
AGGCCCAGGT TTAAGTGTTA TCTCAGCCGA CTGCACACCT TTCTGTCTCT GTCTGGACCT 
CACCTCGGCA CACTGTACAA CAGCTCAGCA CTCGTCAATA CTGGACTGTG GTTCATGCAG 
AAGTGGAAGA AGTCTGGCTC TCTGCTGCAG CTGACCTGTC GAGACCATTC AGACCCGCGT 
CAGACCTTTC TCTATAAGCT GAGCAAGAAA GCAGGCCTTC AGTTCTTCAA GAATGTGGTG 
TTGGTGGGCT CTCTTCAGGA CCGATACGTT CCTTATCACT CCGCTCGAAT CGAGATGTGC 
AAAACAGCCT TAAAGGACAA ACAGACTGGT CCGGTGTATG CTGAGATGAT CGAGAACCTT 
CTCCTTCCTG TGCTTCAGAA CAAAGACTGT AACCTGGTGC GTTACGACGT CATCCACGCG 
CTGCCCAACA CCGCCAACTC TCTGATCGGC CGCGCAGCTC ACATCGCCGT CCTGGACTCT 
GAGATTTTCC TGGAAAAGTT CTTCCTGGTG GCAGGGCTCA AGTTTTTTCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	fam135a
Entrez Gene ID	566435
Full Name	family with sequence similarity 135, member A
Synonyms	im:7140207
General protein information	Preferred Names family with sequence similarity 135, member A Names protein FAM135A
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Homo sapiens (human)	FAM135A	NP_001156001.1
	LOC714862	XP_002803850.1
Gallus gallus (chicken)	FAM135A	XP_419888.2
Danio rerio (zebrafish)	LOC566435	NP_001139066.1
Caenorhabditis elegans (roundworm)	C09D4.4	NP_491607.2
Pan troglodytes (chimpanzee)	FAM135A	XP_001138286.1
Bos taurus (cattle)	FAM135A	XP_002690050.1
Xenopus tropicalis (tropical clawed frog)	fam135a	XP_002939445.1
Canis lupus familiaris (dog)	FAM135A	XP_005627583.1
Mus musculus (house mouse)	Fam135a	NP_080880.4
Rattus norvegicus (Norway rat)	Fam135a	NP_001128069.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

fam135a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

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