ITSN2A cDNA ORF clone, Danio rerio(Zebrafish)

The following ITSN2A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ITSN2A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa150656 XM_021468490.1
Latest version!
Danio rerio intersectin 2a (itsn2a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa56579 XM_017352508.1
Latest version!
Danio rerio intersectin 2a (itsn2a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa01683 NM_001144800.1
Latest version!
Danio rerio intersectin 2a (itsn2a), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa150654 XM_021468487.1
Latest version!
Danio rerio intersectin 2a (itsn2a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa150654 XM_021468488.1
Latest version!
Danio rerio intersectin 2a (itsn2a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
ODa150655 XM_021468489.1
Latest version!
Danio rerio intersectin 2a (itsn2a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
ODa44865 XM_009300808.2
Latest version!
Danio rerio intersectin 2a (itsn2a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa44866 XM_009300809.3
Latest version!
Danio rerio intersectin 2a (itsn2a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa44867 XM_009300811.2
Latest version!
Danio rerio intersectin 2a (itsn2a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
View Old Accession Versions >>
ODa44866 XM_009300809.2 Danio rerio intersectin 2a (itsn2a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa150656
    Clone ID Related Accession (Same CDS sequence) XM_021468490.1
    Accession Version XM_021468490.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3321bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product intersectin 2a isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCAGCGGC AGTTGAATCA GCTGGGCCCT GAAAAGCAGC GACTGGCTGA CAGACTTGTA 
    CATTTTACCC AAAACATCTC CAGTCCTTTG ATAAACGATG TGAAACAGAG TGTGTGCGAG
    AAAGATCGGA GCTGTCGGAA GCTTAAGGAG CAGCTGGATG TTTTGGAGAG AGAAACCACG
    GCCAAACTCT CACAAATGGA GCAGTACAAC AAAGAAATCA AGGAATTGAG ACAGAAGCAG
    AGCCAGCAGC AGGACGTCCT GGAGCAGCTC AGGAGGGTCA GATCTGACAA ACTCAAAGAG
    CTCCAGAGAC ACAGGAAAGA GGAAGAGGAG AGAAAGAGGA AGAAAGAAGA GGAGGCCGCC
    AGACAGGCCC AGTTGGAGCA GGAGCGGCTG GAGCAGGAGG AGGCTGAGCG GCAAAGGAAA
    CTTGCACAGG AACGAGAGGC CAAACTCAGG GAAGAGCAGC AGCGGCAGGC TCTGGCCAGA
    CTGCTGGAGG CTCAGGAGCA GGCCAGAGCC AAAGAGGAGA AGAGGAGAGC TGAGGAGAAG
    GAGAGGAATG AGCTAGATGT ACACACTCAA TCCAGTCTGC CTTTCAACAG CACAGAGAAC
    AACATTTTGC AGCCAGTAGT GATCCCTGAA GTCACGTGTA GTTCTCTGAC CACCTACAGA
    GCTCTCTATC CCTTCACTGC ACGCAACTCA GACGAGCTCA CGCTGGAGGC AGACTGTTTC
    ATCCAGGTGG ATGAATCGAC AGTCAGAGAG ACCGGCTGGC TGTATGGCAG TTACAGTGGA
    AACAGAGGCT GGTTTCCAGA GAGCTATGCT GAGAGATGCA GTAAAGACAG TCAGACTCAA
    CCGACCGCAG CTGAAACTGA GTCCACTGCG CCCTCTGCCA ACTACCCTGG AATTCCTCGA
    GTGGACATAG AAGGACCAAC ACCGACACAC ACCCCAGTAT CCACAAACAC ACAGCACTCA
    CAGGCTGTTG CTCTGTGTGA GTGGAGCGCT AAGACTGACA GTCACCTGGG CTTCTGCAAG
    GACGACATTA TTACAGTTCT GGAGAAGCAG GAGAACTGGT GGTACGGAGA ACTCAAGGAG
    AAACGGGGCT GGTTCCCCTG TTCACATGTC TCTGTGGTCA CCACCAATAA TACACAGAGC
    GAGCCTCTGT ATTCTAGCGT GGAGGAGATT GAAGTGTCAG ACTCTGGTTT GCTTGATGAG
    TATGTGGCTC TGTACACGTA TGAGAGTCCG GAGTCAGGAG ACCTGACGTT CAGTGCAGAG
    GATGTGGTTC TGGTTACAGA GAAAGAAGGA GAGTGGTGGA GAGGCTGTAT TGGTGACCAG
    ACCGGCCTTT TCCCCTCCAA CTATGTTAAA CCCAAAGAAC CTGATACTGC CAGTTCTGCA
    GTTCCCAGTA AAAAGCCAGA AATTGCTCAG GTGACTACAG TCAGTCCTGC AACAACACCA
    GAGCAGCTGA GTCTGACACC TGGCCAGCTG ATCGTGGTGC TGCATAAGAA CTCAAACAGC
    TGGTGGCTTG GTGAACTACA GGCACGGGGT AAGAAGCGTA AAAAGGGCTG GTTTCAATCT
    TCTAATGTCC GATTACTGGA GGCCAACAGC GGCAAAATAA CACCTGCACC TCAACCATTG
    TGTCAGGTTA TTGCAATGTA TGACTACAAA GCAGCCAATA AGGACGAGAT GAGCTTCCAG
    AAAGGTCAGC TGATCACCGT ACTGAACAAG GACAACCCTG ACTGGTGGAA AGGAGAGGTC
    GCAGGGGTCA CAGGGCTGTT TCCAACTAAT TATGTGAAGA TGACCACAGA ATGTGATCCA
    AGCCAGCAAT GGTGTGCTGA CCTGCTTAGT TTAGACTCTA TGTGCACTGA GGAGAGGAAG
    AGGCAAGGCT ACATCCATGA GCTCATTCAG ACAGAAGAGT CTTACCTGGA GGACCTGGAG
    CTGGCAGTGG AGGTGTTTTA TAAGCCAATG GCAGAGTCGG GACGGCTGAC CGAGGCTGAG
    ATCAGCATGA TCTTTGTGAA CTGGCGTGAA TTGATCATGT GCAGCACCAA ACTCCTAAAA
    GCTCTGCGTG TGCGTAAGAA GACAGCAGGT GAGCGAATGC CCGTGCAGGT GGTTGGTGAT
    ATCTTGTCCT CTGAGCTCTC TCACATGCAG GCTTACATTC GCTTCTGCAG CTGCCAGCTC
    AACGCAGCTG CTCTGTTGCA GCAGAAAACA GACAAATCAC TCGACTTCAA ACTCTTCCTC
    AAGAAAATTG CCAGTAATTA CCGCTGTAAA GGAATGCCAC TGTCCAGCTT TCTCCTGAAG
    CCCATGCAGA GGATCACACG ATACCCACTG CTGATCAAAA ACATTCTGGA AAACACTCCT
    CCGGCTCACG CTGACCACTC CAATCTGCAG GCGGCGCTGG AGCAGGCGGA GGAGCTTTGC
    TCACAGGTGA ACGAGGCTGT GAGGGAGAAA GAGAACTCCG ACCGACTGGA GTGGATCCAG
    AACCACGTGT TGTGTGACGG AGTCATTGAG CACCTTGTTT TTAACTCTCT GACGAACTGC
    CTGGGCCCTC GAAAACTGCT GCACAGCGGC AAACTGCATA AGACCAAGAG CAGCAAAGAG
    CTCTGGGCCC TGCTGTTCAA TGACTTCCTG CTCCTCACAT ACACCTCCAA ACAGTTCTCA
    TCCAGCCCAG ACAAACTCTT CAATCCCAAC TCCAACACAC AGTACAAGAT GTACAAGACG
    CCAGTGTTTC TGAATGAGGT CTTGGTGAAA ATGCCCTCTG ACCCGTCCAG CGATGACCCA
    GTTTTCCACA TTTCACACAT TGATCGTGTT TACACACTCA AGACTGACAC TATCAATGAG
    AGGACGACGT GGGTGCAGAA GATCAAAGCA GCATCTGATC ACTTTATAGA GACGGAGAAG
    CTGAAGAGAG AGAAGGCGTA TCAAGCTCGC TCTCAGAAGA ACAGTGGCAT CGGTCGACTG
    CTGGTAACAG TGCTGGAAGC CACAGAGCTG AAGCCCTGCA AACCCAACGG GAAGAGTAAC
    CCATACTGTG AACTAACCAT GGGCGCCCAG TGCTACACGT CCCGGCATCA GCCCGACACG
    CTCAACCCAA AATGGAGCTT CAACTGCCAG TTCTTCATCA AAGACTTGTA CCAGGATGTC
    CTGTGCCTCA CTGTCTTTGA GAGGGACCAG TTCTCTCCTG ATGATTTTCT AGGTCGAACT
    GAAGTTCCTG TAGCGACAAT AAAAAAAGAT CAAGATGGTA AAGGGCCACT GGTTCGACGT
    CTGCTGCTTC ATGAAGTCCC GACTGGAGAG GTTAAAGTGA GGCTTGATCT GCAGCTCTAT
    GAACAAACAC CTCACGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324165.1
    CDS284..3604
    Translation

    Target ORF information:

    RefSeq Version XM_021468490.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio intersectin 2a (itsn2a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468490.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCAGCGGC AGTTGAATCA GCTGGGCCCT GAAAAGCAGC GACTGGCTGA CAGACTTGTA 
    CATTTTACCC AAAACATCTC CAGTCCTTTG ATAAACGATG TGAAACAGAG TGTGTGCGAG
    AAAGATCGGA GCTGTCGGAA GCTTAAGGAG CAGCTGGATG TTTTGGAGAG AGAAACCACG
    GCCAAACTCT CACAAATGGA GCAGTACAAC AAAGAAATCA AGGAATTGAG ACAGAAGCAG
    AGCCAGCAGC AGGACGTCCT GGAGCAGCTC AGGAGGGTCA GATCTGACAA ACTCAAAGAG
    CTCCAGAGAC ACAGGAAAGA GGAAGAGGAG AGAAAGAGGA AGAAAGAAGA GGAGGCCGCC
    AGACAGGCCC AGTTGGAGCA GGAGCGGCTG GAGCAGGAGG AGGCTGAGCG GCAAAGGAAA
    CTTGCACAGG AACGAGAGGC CAAACTCAGG GAAGAGCAGC AGCGGCAGGC TCTGGCCAGA
    CTGCTGGAGG CTCAGGAGCA GGCCAGAGCC AAAGAGGAGA AGAGGAGAGC TGAGGAGAAG
    GAGAGGAATG AGCTAGATGT ACACACTCAA TCCAGTCTGC CTTTCAACAG CACAGAGAAC
    AACATTTTGC AGCCAGTAGT GATCCCTGAA GTCACGTGTA GTTCTCTGAC CACCTACAGA
    GCTCTCTATC CCTTCACTGC ACGCAACTCA GACGAGCTCA CGCTGGAGGC AGACTGTTTC
    ATCCAGGTGG ATGAATCGAC AGTCAGAGAG ACCGGCTGGC TGTATGGCAG TTACAGTGGA
    AACAGAGGCT GGTTTCCAGA GAGCTATGCT GAGAGATGCA GTAAAGACAG TCAGACTCAA
    CCGACCGCAG CTGAAACTGA GTCCACTGCG CCCTCTGCCA ACTACCCTGG AATTCCTCGA
    GTGGACATAG AAGGACCAAC ACCGACACAC ACCCCAGTAT CCACAAACAC ACAGCACTCA
    CAGGCTGTTG CTCTGTGTGA GTGGAGCGCT AAGACTGACA GTCACCTGGG CTTCTGCAAG
    GACGACATTA TTACAGTTCT GGAGAAGCAG GAGAACTGGT GGTACGGAGA ACTCAAGGAG
    AAACGGGGCT GGTTCCCCTG TTCACATGTC TCTGTGGTCA CCACCAATAA TACACAGAGC
    GAGCCTCTGT ATTCTAGCGT GGAGGAGATT GAAGTGTCAG ACTCTGGTTT GCTTGATGAG
    TATGTGGCTC TGTACACGTA TGAGAGTCCG GAGTCAGGAG ACCTGACGTT CAGTGCAGAG
    GATGTGGTTC TGGTTACAGA GAAAGAAGGA GAGTGGTGGA GAGGCTGTAT TGGTGACCAG
    ACCGGCCTTT TCCCCTCCAA CTATGTTAAA CCCAAAGAAC CTGATACTGC CAGTTCTGCA
    GTTCCCAGTA AAAAGCCAGA AATTGCTCAG GTGACTACAG TCAGTCCTGC AACAACACCA
    GAGCAGCTGA GTCTGACACC TGGCCAGCTG ATCGTGGTGC TGCATAAGAA CTCAAACAGC
    TGGTGGCTTG GTGAACTACA GGCACGGGGT AAGAAGCGTA AAAAGGGCTG GTTTCAATCT
    TCTAATGTCC GATTACTGGA GGCCAACAGC GGCAAAATAA CACCTGCACC TCAACCATTG
    TGTCAGGTTA TTGCAATGTA TGACTACAAA GCAGCCAATA AGGACGAGAT GAGCTTCCAG
    AAAGGTCAGC TGATCACCGT ACTGAACAAG GACAACCCTG ACTGGTGGAA AGGAGAGGTC
    GCAGGGGTCA CAGGGCTGTT TCCAACTAAT TATGTGAAGA TGACCACAGA ATGTGATCCA
    AGCCAGCAAT GGTGTGCTGA CCTGCTTAGT TTAGACTCTA TGTGCACTGA GGAGAGGAAG
    AGGCAAGGCT ACATCCATGA GCTCATTCAG ACAGAAGAGT CTTACCTGGA GGACCTGGAG
    CTGGCAGTGG AGGTGTTTTA TAAGCCAATG GCAGAGTCGG GACGGCTGAC CGAGGCTGAG
    ATCAGCATGA TCTTTGTGAA CTGGCGTGAA TTGATCATGT GCAGCACCAA ACTCCTAAAA
    GCTCTGCGTG TGCGTAAGAA GACAGCAGGT GAGCGAATGC CCGTGCAGGT GGTTGGTGAT
    ATCTTGTCCT CTGAGCTCTC TCACATGCAG GCTTACATTC GCTTCTGCAG CTGCCAGCTC
    AACGCAGCTG CTCTGTTGCA GCAGAAAACA GACAAATCAC TCGACTTCAA ACTCTTCCTC
    AAGAAAATTG CCAGTAATTA CCGCTGTAAA GGAATGCCAC TGTCCAGCTT TCTCCTGAAG
    CCCATGCAGA GGATCACACG ATACCCACTG CTGATCAAAA ACATTCTGGA AAACACTCCT
    CCGGCTCACG CTGACCACTC CAATCTGCAG GCGGCGCTGG AGCAGGCGGA GGAGCTTTGC
    TCACAGGTGA ACGAGGCTGT GAGGGAGAAA GAGAACTCCG ACCGACTGGA GTGGATCCAG
    AACCACGTGT TGTGTGACGG AGTCATTGAG CACCTTGTTT TTAACTCTCT GACGAACTGC
    CTGGGCCCTC GAAAACTGCT GCACAGCGGC AAACTGCATA AGACCAAGAG CAGCAAAGAG
    CTCTGGGCCC TGCTGTTCAA TGACTTCCTG CTCCTCACAT ACACCTCCAA ACAGTTCTCA
    TCCAGCCCAG ACAAACTCTT CAATCCCAAC TCCAACACAC AGTACAAGAT GTACAAGACG
    CCAGTGTTTC TGAATGAGGT CTTGGTGAAA ATGCCCTCTG ACCCGTCCAG CGATGACCCA
    GTTTTCCACA TTTCACACAT TGATCGTGTT TACACACTCA AGACTGACAC TATCAATGAG
    AGGACGACGT GGGTGCAGAA GATCAAAGCA GCATCTGATC ACTTTATAGA GACGGAGAAG
    CTGAAGAGAG AGAAGGCGTA TCAAGCTCGC TCTCAGAAGA ACAGTGGCAT CGGTCGACTG
    CTGGTAACAG TGCTGGAAGC CACAGAGCTG AAGCCCTGCA AACCCAACGG GAAGAGTAAC
    CCATACTGTG AACTAACCAT GGGCGCCCAG TGCTACACGT CCCGGCATCA GCCCGACACG
    CTCAACCCAA AATGGAGCTT CAACTGCCAG TTCTTCATCA AAGACTTGTA CCAGGATGTC
    CTGTGCCTCA CTGTCTTTGA GAGGGACCAG TTCTCTCCTG ATGATTTTCT AGGTCGAACT
    GAAGTTCCTG TAGCGACAAT AAAAAAAGAT CAAGATGGTA AAGGGCCACT GGTTCGACGT
    CTGCTGCTTC ATGAAGTCCC GACTGGAGAG GTTAAAGTGA GGCTTGATCT GCAGCTCTAT
    GAACAAACAC CTCACGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56579
    Clone ID Related Accession (Same CDS sequence) XM_017352508.1
    Accession Version XM_017352508.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3171bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product intersectin 2a isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGATGACT GTGTGCTACA AGGACTTCTC TCTTTGCTGG GCTGCTTAAC CAATCTCTTC 
    CTTCTAATAA AGGAATTGAG ACAGAAGCAG AGCCAGCAGC AGGACGTCCT GGAGCAGCTC
    AGGAGGGTCA GATCTGACAA ACTCAAAGAG CTCCAGAGAC ACAGGAAAGA GGAAGAGGAG
    AGAAAGAGGA AGAAAGAAGA GGAGGCCGCC AGACAGGCCC AGTTGGAGCA GGAGCGGCTG
    GAGCAGGAGG AGGCTGAGCG GCAAAGGAAA CTTGCACAGG AACGAGAGGC CAAACTCAGG
    GAAGAGCAGC AGCGGCAGGC TCTGGCCAGA CTGCTGGAGG CTCAGGAGCA GGCCAGAGCC
    AAAGAGGAGA AGAGGAGAGC TGAGGAGAAG GAGAGGAATG AGCTAGATGT ACACACTCAA
    TCCAGTCTGC CTTTCAACAG CACAGAGAAC AACATTTTGC AGCCAGTAGT GATCCCTGAA
    GTCACGTGTA GTTCTCTGAC CACCTACAGA GCTCTCTATC CCTTCACTGC ACGCAACTCA
    GACGAGCTCA CGCTGGAGGC AGACTGTTTC ATCCAGGTGG ATGAATCGAC AGTCAGAGAG
    ACCGGCTGGC TGTATGGCAG TTACAGTGGA AACAGAGGCT GGTTTCCAGA GAGCTATGCT
    GAGAGATGCA GTAAAGACAG TCAGACTCAA CCGACCGCAG CTGAAACTGA GTCCACTGCG
    CCCTCTGCCA ACTACCCTGG AATTCCTCGA GTGGACATAG AAGGACCAAC ACCGACACAC
    ACCCCAGTAT CCACAAACAC ACAGCACTCA CAGGCTGTTG CTCTGTGTGA GTGGAGCGCT
    AAGACTGACA GTCACCTGGG CTTCTGCAAG GACGACATTA TTACAGTTCT GGAGAAGCAG
    GAGAACTGGT GGTACGGAGA ACTCAAGGAG AAACGGGGCT GGTTCCCCTG TTCACATGTC
    TCTGTGGTCA CCACCAATAA TACACAGAGC GAGCCTCTGT ATTCTAGCGT GGAGGAGATT
    GAAGTGTCAG ACTCTGGTTT GCTTGATGAG TATGTGGCTC TGTACACGTA TGAGAGTCCG
    GAGTCAGGAG ACCTGACGTT CAGTGCAGAG GATGTGGTTC TGGTTACAGA GAAAGAAGGA
    GAGTGGTGGA GAGGCTGTAT TGGTGACCAG ACCGGCCTTT TCCCCTCCAA CTATGTTAAA
    CCCAAAGAAC CTGATACTGC CAGTTCTGCA GTTCCCAGTA AAAAGCCAGA AATTGCTCAG
    GTGACTACAG TCAGTCCTGC AACAACACCA GAGCAGCTGA GTCTGACACC TGGCCAGCTG
    ATCGTGGTGC TGCATAAGAA CTCAAACAGC TGGTGGCTTG GTGAACTACA GGCACGGGGT
    AAGAAGCGTA AAAAGGGCTG GTTTCAATCT TCTAATGTCC GATTACTGGA GGCCAACAGC
    GGCAAAATAA CACCTGCACC TCAACCATTG TGTCAGGTTA TTGCAATGTA TGACTACAAA
    GCAGCCAATA AGGACGAGAT GAGCTTCCAG AAAGGTCAGC TGATCACCGT ACTGAACAAG
    GACAACCCTG ACTGGTGGAA AGGAGAGGTC GCAGGGGTCA CAGGGCTGTT TCCAACTAAT
    TATGTGAAGA TGACCACAGA ATGTGATCCA AGCCAGCAAT GGTGTGCTGA CCTGCTTAGT
    TTAGACTCTA TGTGCACTGA GGAGAGGAAG AGGCAAGGCT ACATCCATGA GCTCATTCAG
    ACAGAAGAGT CTTACCTGGA GGACCTGGAG CTGGCAGTGG AGGTGTTTTA TAAGCCAATG
    GCAGAGTCGG GACGGCTGAC CGAGGCTGAG ATCAGCATGA TCTTTGTGAA CTGGCGTGAA
    TTGATCATGT GCAGCACCAA ACTCCTAAAA GCTCTGCGTG TGCGTAAGAA GACAGCAGGT
    GAGCGAATGC CCGTGCAGGT GGTTGGTGAT ATCTTGTCCT CTGAGCTCTC TCACATGCAG
    GCTTACATTC GCTTCTGCAG CTGCCAGCTC AACGCAGCTG CTCTGTTGCA GCAGAAAACA
    GACAAATCAC TCGACTTCAA ACTCTTCCTC AAGAAAATTG CCAGTAATTA CCGCTGTAAA
    GGAATGCCAC TGTCCAGCTT TCTCCTGAAG CCCATGCAGA GGATCACACG ATACCCACTG
    CTGATCAAAA ACATTCTGGA AAACACTCCT CCGGCTCACG CTGACCACTC CAATCTGCAG
    GCGGCGCTGG AGCAGGCGGA GGAGCTTTGC TCACAGGTGA ACGAGGCTGT GAGGGAGAAA
    GAGAACTCCG ACCGACTGGA GTGGATCCAG AACCACGTGT TGTGTGACGG AGTCATTGAG
    CACCTTGTTT TTAACTCTCT GACGAACTGC CTGGGCCCTC GAAAACTGCT GCACAGCGGC
    AAACTGCATA AGACCAAGAG CAGCAAAGAG CTCTGGGCCC TGCTGTTCAA TGACTTCCTG
    CTCCTCACAT ACACCTCCAA ACAGTTCTCA TCCAGCCCAG ACAAACTCTT CAATCCCAAC
    TCCAACACAC AGTACAAGAT GTACAAGACG CCAGTGTTTC TGAATGAGGT CTTGGTGAAA
    ATGCCCTCTG ACCCGTCCAG CGATGACCCA GTTTTCCACA TTTCACACAT TGATCGTGTT
    TACACACTCA AGACTGACAC TATCAATGAG AGGACGACGT GGGTGCAGAA GATCAAAGCA
    GCATCTGATC ACTTTATAGA GACGGAGAAG CTGAAGAGAG AGAAGGCGTA TCAAGCTCGC
    TCTCAGAAGA ACAGTGGCAT CGGTCGACTG CTGGTAACAG TGCTGGAAGC CACAGAGCTG
    AAGCCCTGCA AACCCAACGG GAAGAGTAAC CCATACTGTG AACTAACCAT GGGCGCCCAG
    TGCTACACGT CCCGGCATCA GCCCGACACG CTCAACCCAA AATGGAGCTT CAACTGCCAG
    TTCTTCATCA AAGACTTGTA CCAGGATGTC CTGTGCCTCA CTGTCTTTGA GAGGGACCAG
    TTCTCTCCTG ATGATTTTCT AGGTCGAACT GAAGTTCCTG TAGCGACAAT AAAAAAAGAT
    CAAGATGGTA AAGGGCCACT GGTTCGACGT CTGCTGCTTC ATGAAGTCCC GACTGGAGAG
    GTTAAAGTGA GGCTTGATCT GCAGCTCTAT GAACAAACAC CTCACGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017207997.1
    CDS16..3186
    Translation

    Target ORF information:

    RefSeq Version XM_017352508.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio intersectin 2a (itsn2a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017352508.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGATGACT GTGTGCTACA AGGACTTCTC TCTTTGCTGG GCTGCTTAAC CAATCTCTTC 
    CTTCTAATAA AGGAATTGAG ACAGAAGCAG AGCCAGCAGC AGGACGTCCT GGAGCAGCTC
    AGGAGGGTCA GATCTGACAA ACTCAAAGAG CTCCAGAGAC ACAGGAAAGA GGAAGAGGAG
    AGAAAGAGGA AGAAAGAAGA GGAGGCCGCC AGACAGGCCC AGTTGGAGCA GGAGCGGCTG
    GAGCAGGAGG AGGCTGAGCG GCAAAGGAAA CTTGCACAGG AACGAGAGGC CAAACTCAGG
    GAAGAGCAGC AGCGGCAGGC TCTGGCCAGA CTGCTGGAGG CTCAGGAGCA GGCCAGAGCC
    AAAGAGGAGA AGAGGAGAGC TGAGGAGAAG GAGAGGAATG AGCTAGATGT ACACACTCAA
    TCCAGTCTGC CTTTCAACAG CACAGAGAAC AACATTTTGC AGCCAGTAGT GATCCCTGAA
    GTCACGTGTA GTTCTCTGAC CACCTACAGA GCTCTCTATC CCTTCACTGC ACGCAACTCA
    GACGAGCTCA CGCTGGAGGC AGACTGTTTC ATCCAGGTGG ATGAATCGAC AGTCAGAGAG
    ACCGGCTGGC TGTATGGCAG TTACAGTGGA AACAGAGGCT GGTTTCCAGA GAGCTATGCT
    GAGAGATGCA GTAAAGACAG TCAGACTCAA CCGACCGCAG CTGAAACTGA GTCCACTGCG
    CCCTCTGCCA ACTACCCTGG AATTCCTCGA GTGGACATAG AAGGACCAAC ACCGACACAC
    ACCCCAGTAT CCACAAACAC ACAGCACTCA CAGGCTGTTG CTCTGTGTGA GTGGAGCGCT
    AAGACTGACA GTCACCTGGG CTTCTGCAAG GACGACATTA TTACAGTTCT GGAGAAGCAG
    GAGAACTGGT GGTACGGAGA ACTCAAGGAG AAACGGGGCT GGTTCCCCTG TTCACATGTC
    TCTGTGGTCA CCACCAATAA TACACAGAGC GAGCCTCTGT ATTCTAGCGT GGAGGAGATT
    GAAGTGTCAG ACTCTGGTTT GCTTGATGAG TATGTGGCTC TGTACACGTA TGAGAGTCCG
    GAGTCAGGAG ACCTGACGTT CAGTGCAGAG GATGTGGTTC TGGTTACAGA GAAAGAAGGA
    GAGTGGTGGA GAGGCTGTAT TGGTGACCAG ACCGGCCTTT TCCCCTCCAA CTATGTTAAA
    CCCAAAGAAC CTGATACTGC CAGTTCTGCA GTTCCCAGTA AAAAGCCAGA AATTGCTCAG
    GTGACTACAG TCAGTCCTGC AACAACACCA GAGCAGCTGA GTCTGACACC TGGCCAGCTG
    ATCGTGGTGC TGCATAAGAA CTCAAACAGC TGGTGGCTTG GTGAACTACA GGCACGGGGT
    AAGAAGCGTA AAAAGGGCTG GTTTCAATCT TCTAATGTCC GATTACTGGA GGCCAACAGC
    GGCAAAATAA CACCTGCACC TCAACCATTG TGTCAGGTTA TTGCAATGTA TGACTACAAA
    GCAGCCAATA AGGACGAGAT GAGCTTCCAG AAAGGTCAGC TGATCACCGT ACTGAACAAG
    GACAACCCTG ACTGGTGGAA AGGAGAGGTC GCAGGGGTCA CAGGGCTGTT TCCAACTAAT
    TATGTGAAGA TGACCACAGA ATGTGATCCA AGCCAGCAAT GGTGTGCTGA CCTGCTTAGT
    TTAGACTCTA TGTGCACTGA GGAGAGGAAG AGGCAAGGCT ACATCCATGA GCTCATTCAG
    ACAGAAGAGT CTTACCTGGA GGACCTGGAG CTGGCAGTGG AGGTGTTTTA TAAGCCAATG
    GCAGAGTCGG GACGGCTGAC CGAGGCTGAG ATCAGCATGA TCTTTGTGAA CTGGCGTGAA
    TTGATCATGT GCAGCACCAA ACTCCTAAAA GCTCTGCGTG TGCGTAAGAA GACAGCAGGT
    GAGCGAATGC CCGTGCAGGT GGTTGGTGAT ATCTTGTCCT CTGAGCTCTC TCACATGCAG
    GCTTACATTC GCTTCTGCAG CTGCCAGCTC AACGCAGCTG CTCTGTTGCA GCAGAAAACA
    GACAAATCAC TCGACTTCAA ACTCTTCCTC AAGAAAATTG CCAGTAATTA CCGCTGTAAA
    GGAATGCCAC TGTCCAGCTT TCTCCTGAAG CCCATGCAGA GGATCACACG ATACCCACTG
    CTGATCAAAA ACATTCTGGA AAACACTCCT CCGGCTCACG CTGACCACTC CAATCTGCAG
    GCGGCGCTGG AGCAGGCGGA GGAGCTTTGC TCACAGGTGA ACGAGGCTGT GAGGGAGAAA
    GAGAACTCCG ACCGACTGGA GTGGATCCAG AACCACGTGT TGTGTGACGG AGTCATTGAG
    CACCTTGTTT TTAACTCTCT GACGAACTGC CTGGGCCCTC GAAAACTGCT GCACAGCGGC
    AAACTGCATA AGACCAAGAG CAGCAAAGAG CTCTGGGCCC TGCTGTTCAA TGACTTCCTG
    CTCCTCACAT ACACCTCCAA ACAGTTCTCA TCCAGCCCAG ACAAACTCTT CAATCCCAAC
    TCCAACACAC AGTACAAGAT GTACAAGACG CCAGTGTTTC TGAATGAGGT CTTGGTGAAA
    ATGCCCTCTG ACCCGTCCAG CGATGACCCA GTTTTCCACA TTTCACACAT TGATCGTGTT
    TACACACTCA AGACTGACAC TATCAATGAG AGGACGACGT GGGTGCAGAA GATCAAAGCA
    GCATCTGATC ACTTTATAGA GACGGAGAAG CTGAAGAGAG AGAAGGCGTA TCAAGCTCGC
    TCTCAGAAGA ACAGTGGCAT CGGTCGACTG CTGGTAACAG TGCTGGAAGC CACAGAGCTG
    AAGCCCTGCA AACCCAACGG GAAGAGTAAC CCATACTGTG AACTAACCAT GGGCGCCCAG
    TGCTACACGT CCCGGCATCA GCCCGACACG CTCAACCCAA AATGGAGCTT CAACTGCCAG
    TTCTTCATCA AAGACTTGTA CCAGGATGTC CTGTGCCTCA CTGTCTTTGA GAGGGACCAG
    TTCTCTCCTG ATGATTTTCT AGGTCGAACT GAAGTTCCTG TAGCGACAAT AAAAAAAGAT
    CAAGATGGTA AAGGGCCACT GGTTCGACGT CTGCTGCTTC ATGAAGTCCC GACTGGAGAG
    GTTAAAGTGA GGCTTGATCT GCAGCTCTAT GAACAAACAC CTCACGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa01683
    Clone ID Related Accession (Same CDS sequence) NM_001144800.1
    Accession Version NM_001144800.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4998bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-23
    Organism Danio rerio(zebrafish)
    Product intersectin 2a
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR792450.4. On May 20, 2009 this sequence version replaced XR_029418.2. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATGGTG GCTTTTCTAT TTGGGCTATA ACTCCAGAAG AGAGGGGGAA ACATGACAAA 
    CAGTTTGACT CGCTGGCTCC CACCCTGGGA TATTTGTCGG GAGATCAAGC AAGAAAGTTC
    TTCCTCCAAT CTGGTCTTCC TACATCAGTC CTGGCAGACA TCTGGGCTCT GGCGGACATA
    GGAAAGGATG GGAAGATGGA CAGATTGGAG TTCTCTATTG CAATGAAACT AATTAAACTG
    CAGCTGCAGG GTCAGCCACT TCCGTCCAGC CTGCCGATCA TCATGAAGCA GACGCCCACC
    CCTGTGATGA CGTCATCGTC ACGCTTCGGT ATGGGCTCCA TGCCAAATCT CTCCGCCATG
    TCGGTGATGC CCATCCTAAC ACCCATCCCC ACATGCCCTG TCATGGCCCC TTTAGTTCCT
    GTGCCCACCC TGATGTCTGG GCCACTGCCT CAGATGCCCA ACTCTATCAG TGTGCCGGCC
    TTACCTAACG GCAATGCAAC TCTCCTCACA CCAACACCAG CATTCCCAGT CTCCAGTGGT
    TTGACTAAAT CTCACTCGCT CCTGGACCTT GGCTCTAGCA GTTCTAACTC GTCCTCCACC
    ACCTCCCTTG CCAGTAACTC CCCGAAAACG AGTAGTTCTG ATTGGGCAGT GCCTCAGTCA
    TCCCGTCTGA AGTACCGACA ACTGTTTAAC AGCCTGGACA AGCTAATGAG TGGATACCTG
    TCAGGTCCCC AGGTCAGAAA TGCCCTCACA GCTTCAAACC TCACACAAAC ACAGCTTGCC
    ACTATATGGT TTCTGGCTGA TGTGGATCGT GATGGGCAGT TGAGAGCAGA AGAGTTTATT
    TTAGCGATGC ATCTGGTTGA AATGGCCAAG ACAGGGCGGC CTTTACCTCT CACTTTACCT
    CCCGACCTTG TGCCTCCTTC ACTCAGAACA ACTAAGTCAT GTGACCTGCT AAACGGACCT
    GTTCCTCTGA TTAGCACTGA GCTGATTGAG CCAGAACTGC CACAGAAAAA CAAGAGCAAT
    GTTTCATATG AAGACAAACT GAGGGAAAAT TTCCAGAGGG GAAACGCAGA GCTGGAGAAG
    CGTCGCCTGG CTCTTCAGGA GCAGCAGAGG AGAGAGGAGG AGCGCAGGAG AGAAGAGGAG
    AGGCGAGAGG AGGAGAAGAG ACAGCAGAAG GAAAGAGAGG AGCGAGAGAG GAGAGAGAGA
    GAAGCGCGAG AAATGGAGAT CAGGAGACAG AGAGAGGAGG AGCGCAGGAT GGAGAGACAG
    AGAGAGCTGG AGCGACAGAG AGAGGAGGAA CGACTGAAAG AATTAGAGCG GCGAGAGGCA
    AGTAGAGCAA GCAAGGACAT ACAGAGGGAA GCTGAGCGAC AAAGACGGAG GGAATGGGAG
    AAGAGCAAAC GTGTGGAGCT TCAGACTCAG AAAGAAAAGG AGCAGAATGA CATACAGAGA
    CTAAAAGACA GGAAGAGGAG TCTTGAGATT GAGCTGGAGG CCGTGGGTAA CAAGCATAAG
    CAGATCTCCA GTCAGCTGCG GGATACTAAG AGTAAGAGAC AGGGGCAGTG GGCCGAGTTG
    GAGCTGATCA ACCAGAGGAG AGACGTCTGC ATTACTGATA TCAACTCACT GCAGTTACAG
    TTTGATGAGA TGCAGCGGCA GTTGAATCAG CTGGGCCCTG AAAAGCAGCG ACTGGCTGAC
    AGACTTGTAC ATTTTACCCA AAACATCTCC AGTCCTTTGA TAAACGATGT GAAACAGAGT
    GTGTGCGAGA AAGATCGGAG CTGTCGGAAG CTGAAGGAGC AGCTGGATGT TTTGGAGAGA
    GAAACCACGG CCAAACTCTC ACAAATGGAG CAGTACAACA AAGAAATCAA GGAATTGAGA
    CAGAAGCAGA GCCAGCAGCA GGACGTCCTG GAGCAGCTCA GGAGGGTCAG ATCTGACAAA
    CTCAAAGAGC TCCAGAGACA CAGGAAAGAG GAAGAGGAGA GAAAGAGGAA GAAAGAGGAG
    GAGGCCGCCA GACAGGCCCA GTTGGAGCAG GAGCGGCTGG AGCAGGAGGA GGCTGAGCGG
    CAAAGGAAAC TTGCACAGGA ACGAGAGGCC AAACTCAGGG AAGAGCAGCA GCGGCAGGCT
    CTGGCCAGAC TGCTGGAGGC CCAGGAGCAG GCCAGAGCCA AAGAGGAGAA GAGGAGAGCT
    GAGGAGAAGG AGAGGAATGA GCTAGATGTA CACACTCAAT CCAGTCTGCC TTTCAACAGC
    ACAGAAAACA ACATTTTGCA GCCAGTAGTG ATCCCTGAAG TCACGTGTAG TTCTCTGACC
    ACCTACAGAG CTCTCTATCC CTTCACTGCA CGCAACTCAG ACGAGCTCAC GCTGGAGGCA
    GACTGTTTCA TCCAGGTGGA TGAATCGACA GTCAGAGAGA CCGGCTGGCT GTATGGTAGT
    TACAGTGGAA ACAGAGGCTG GTTTCCAGAG AGCTATGCTG AGAGATGCAG TAAAGACAGT
    CAGACTCAAC CGACCGCAGC TGAAACTGAG TCCACTGCGC CCTCTGCCAA CTACCCTGGA
    ATTCCTCGAG TGGACATAGA AGGACCAACA CCGACACACA CCCCAGTATC CACAAACACA
    CAGCACTCAC AGGCTGTTGC TCTGTGTGAG TGGAGCGCTA AGACTGACAG TCACCTGGGC
    TTCTGCAAGG ACGACATTAT TACAGTTCTG GAGAAGCAGG AGAACTGGTG GTACGGAGAA
    CTCAAGGAGA AACGGGGCTG GTTCCCCTGT TCACATGTCT CTGTGGTCAC CACCAATAAC
    ACACAGAGCG ATTTTGACAT GCCTGCTGGA AATCTTTTAA GTGTTTTGGT ATCTCTTTTA
    GGCCTCTGTA TTCTAGCGTG GAGGAGATTG AAGTGTCAGA CTCTGGTTTG CTTGATGTAT
    GTGGCTCTGT ACACGTATGA GAGTCCGGAG TCAGGAGACC TGACGTTCAG TGCAGAGGAT
    GTGGTTCTGG TTACAGAGAA AGAAGGAGAG TGGTGGAGAG GCTGTATTGG TGACCAGACC
    GGCCTTTTCC CCTCCAACTA TGTTAAACCC AAAGAACCTG ATACTGCCAG TTCTGCAGTT
    CCCAGTAAAA AGCCAGAAAT TGCTCAGGTG ACTACAGTCA GTCCTGCAAC AACACCAGAG
    CAGCTGAGTC TGACACCTGG CCAGCTGATC GTGGTGCTGC ATAAGAACTC AAACAGCTGG
    TGGCTTGGTG AACTACAGGC ACGGGGTAAG AAGCGTAAAA AGGGCTGGTT TCAATCTTCT
    AATGTCCGAT TACTGGAGGC CAACAGCGGC AAAATAACAC CTGCACCTCA ACCATTGTGT
    CAGGTTATTG CAATGTATGA CTACAAAGCA GCCAATAAGG ACGAGATGAG CTTCCAGAAA
    GGTCAGCTGA TCACCGTACT GAACAAGGAC AACCCTGACT GGTGGAAAGG AGAGGTCGCA
    GGGCTCACAG GGCTGTTTCC AACTAATTAT GTGAAGATGA CCACAGAATG TGATCCAAGC
    CAGCAATGGT GTGCTGACCT GCTTAGTTTA GACTCTATGT GCACTGAGGA GAGGAAGAGG
    CAAGGCTACA TCCATGAGCT CATTCAGACA GAAGAGTCTT ACCTGGAGGA CCTGGAGCTG
    GCAGTGGAGG TGTTTTATAA GCCAATGGCA GAGTCGGGAC GGCTGACTGA GGCTGAGATG
    AGCATGATCT TTGTGAACTG GCGTGAATTG ATCATGTGCA GCACCAAACT CCTAAAAGCT
    CTGCGTGTGC GTAAGAAGAC AGCAGGTGAG CGAATGCCCG TGCAGGTGGT TGGTGATATC
    TTGTCCTCTG AGCTCTCTCA CATGCAGGCC TACATTCGCT TCTGCAGCTG CCAGCTCAAC
    GCAGCCGCTC TGCTGCAGCA GAAAACAGAC AAATCACTCG ACTTCAAACT CTTCCTCAAG
    AAAATTGCCA GTAATTACCG CTGTAAAGGA ATGCCACTCT CCAGCTTTCT CCTGAAGCCC
    ATGCAGAGGA TCACACGATA CCCACTGCTG ATCAAAAACA TTCTGGAAAA CACCCCTCCG
    GCTCACGCTG ACCACTCCAA TCTGCAGGCG GCGCTGGAGC AGGCGGAGGA GCTTTGCTCA
    CAGGTGAACG AGGCCGTGAG GGAGAAAGAG AACTCCGACC GACTGGAGTG GATCCAGAAC
    CACGTGTTGT GCGACGGAGT CATTGAGCAC CTTGTTTTTA ACTCTCTGAC GAACTGCCTG
    GGCCCTCGAA AACTGCTGCA CAGTGGCAAA CTGCACAAGA CCAAGAGCAG CAAAGAGCTC
    TGGGCCCTGC TGTTCAATGA CTTCCTGCTC CTCACATACA CCTCCAAACA GTTCTCATCC
    AGCCCAGACA AACTCTTCAA TCCAAACTCC AACACACAGT ACAAGATGTA CAAGACGCCA
    GTGTTTCTGA ATGAGGTCTT GGTGAAAATG CCCTCTGACC CGTCCAGCGA TGATCCAGTT
    TTCCACATTT CACACATTGA TCGTGTTTAC ACACTAAAGA CTGACACTAT CAATGAGAGG
    ACGACGTGGG TGCAGAAGAT CAAAGCAGCA TCTGATCACT TTATAGAGAC GGAGAAGCTG
    AAGAGAGAGA AGGCGTATCA AGCTCGCTCT CAGAAGAACA GTGGCATCGG TCGACTGCTG
    GTAACAGTGT TGGAGGCCAC AGAGCTGAAG CCCTGCAAAC CCAACGGGAA GAGTAACCCA
    TACTGTGAAC TAACCATGGG CGCCCAGTGC TACACGTCCC GGCATCAGCC CGACACGCTC
    AACCCAAAAT GGAGCTTCAA CTGCCAGTTC TTCATCAAAG ACCTGTACCA GGATGTCCTG
    TGCCTCACTG TCTTTGAGAG GGACCAGTTC TCTCCTGATG ATTTTCTAGG TCGAACTGAA
    GTTCCTGTAG CGACAATAAA AAAAGATCAA GATGGTAAAG GGCCACTGGT TCGACGTCTG
    CTGCTTCATG AAGTCCCGAC TGGAGAGGTT AAAGTGAGGC TTGATCTGCA GCTCTATGAA
    CAAACACCTC ACGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001138272.1
    CDS55..5052
    Translation

    Target ORF information:

    RefSeq Version NM_001144800.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio intersectin 2a (itsn2a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001144800.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATGGTG GCTTTTCTAT TTGGGCTATA ACTCCAGAAG AGAGGGGGAA ACATGACAAA 
    CAGTTTGACT CGCTGGCTCC CACCCTGGGA TATTTGTCGG GAGATCAAGC AAGAAAGTTC
    TTCCTCCAAT CTGGTCTTCC TACATCAGTC CTGGCAGACA TCTGGGCTCT GGCGGACATA
    GGAAAGGATG GGAAGATGGA CAGATTGGAG TTCTCTATTG CAATGAAACT AATTAAACTG
    CAGCTGCAGG GTCAGCCACT TCCGTCCAGC CTGCCGATCA TCATGAAGCA GACGCCCACC
    CCTGTGATGA CGTCATCGTC ACGCTTCGGT ATGGGCTCCA TGCCAAATCT CTCCGCCATG
    TCGGTGATGC CCATCCTAAC ACCCATCCCC ACATGCCCTG TCATGGCCCC TTTAGTTCCT
    GTGCCCACCC TGATGTCTGG GCCACTGCCT CAGATGCCCA ACTCTATCAG TGTGCCGGCC
    TTACCTAACG GCAATGCAAC TCTCCTCACA CCAACACCAG CATTCCCAGT CTCCAGTGGT
    TTGACTAAAT CTCACTCGCT CCTGGACCTT GGCTCTAGCA GTTCTAACTC GTCCTCCACC
    ACCTCCCTTG CCAGTAACTC CCCGAAAACG AGTAGTTCTG ATTGGGCAGT GCCTCAGTCA
    TCCCGTCTGA AGTACCGACA ACTGTTTAAC AGCCTGGACA AGCTAATGAG TGGATACCTG
    TCAGGTCCCC AGGTCAGAAA TGCCCTCACA GCTTCAAACC TCACACAAAC ACAGCTTGCC
    ACTATATGGT TTCTGGCTGA TGTGGATCGT GATGGGCAGT TGAGAGCAGA AGAGTTTATT
    TTAGCGATGC ATCTGGTTGA AATGGCCAAG ACAGGGCGGC CTTTACCTCT CACTTTACCT
    CCCGACCTTG TGCCTCCTTC ACTCAGAACA ACTAAGTCAT GTGACCTGCT AAACGGACCT
    GTTCCTCTGA TTAGCACTGA GCTGATTGAG CCAGAACTGC CACAGAAAAA CAAGAGCAAT
    GTTTCATATG AAGACAAACT GAGGGAAAAT TTCCAGAGGG GAAACGCAGA GCTGGAGAAG
    CGTCGCCTGG CTCTTCAGGA GCAGCAGAGG AGAGAGGAGG AGCGCAGGAG AGAAGAGGAG
    AGGCGAGAGG AGGAGAAGAG ACAGCAGAAG GAAAGAGAGG AGCGAGAGAG GAGAGAGAGA
    GAAGCGCGAG AAATGGAGAT CAGGAGACAG AGAGAGGAGG AGCGCAGGAT GGAGAGACAG
    AGAGAGCTGG AGCGACAGAG AGAGGAGGAA CGACTGAAAG AATTAGAGCG GCGAGAGGCA
    AGTAGAGCAA GCAAGGACAT ACAGAGGGAA GCTGAGCGAC AAAGACGGAG GGAATGGGAG
    AAGAGCAAAC GTGTGGAGCT TCAGACTCAG AAAGAAAAGG AGCAGAATGA CATACAGAGA
    CTAAAAGACA GGAAGAGGAG TCTTGAGATT GAGCTGGAGG CCGTGGGTAA CAAGCATAAG
    CAGATCTCCA GTCAGCTGCG GGATACTAAG AGTAAGAGAC AGGGGCAGTG GGCCGAGTTG
    GAGCTGATCA ACCAGAGGAG AGACGTCTGC ATTACTGATA TCAACTCACT GCAGTTACAG
    TTTGATGAGA TGCAGCGGCA GTTGAATCAG CTGGGCCCTG AAAAGCAGCG ACTGGCTGAC
    AGACTTGTAC ATTTTACCCA AAACATCTCC AGTCCTTTGA TAAACGATGT GAAACAGAGT
    GTGTGCGAGA AAGATCGGAG CTGTCGGAAG CTGAAGGAGC AGCTGGATGT TTTGGAGAGA
    GAAACCACGG CCAAACTCTC ACAAATGGAG CAGTACAACA AAGAAATCAA GGAATTGAGA
    CAGAAGCAGA GCCAGCAGCA GGACGTCCTG GAGCAGCTCA GGAGGGTCAG ATCTGACAAA
    CTCAAAGAGC TCCAGAGACA CAGGAAAGAG GAAGAGGAGA GAAAGAGGAA GAAAGAGGAG
    GAGGCCGCCA GACAGGCCCA GTTGGAGCAG GAGCGGCTGG AGCAGGAGGA GGCTGAGCGG
    CAAAGGAAAC TTGCACAGGA ACGAGAGGCC AAACTCAGGG AAGAGCAGCA GCGGCAGGCT
    CTGGCCAGAC TGCTGGAGGC CCAGGAGCAG GCCAGAGCCA AAGAGGAGAA GAGGAGAGCT
    GAGGAGAAGG AGAGGAATGA GCTAGATGTA CACACTCAAT CCAGTCTGCC TTTCAACAGC
    ACAGAAAACA ACATTTTGCA GCCAGTAGTG ATCCCTGAAG TCACGTGTAG TTCTCTGACC
    ACCTACAGAG CTCTCTATCC CTTCACTGCA CGCAACTCAG ACGAGCTCAC GCTGGAGGCA
    GACTGTTTCA TCCAGGTGGA TGAATCGACA GTCAGAGAGA CCGGCTGGCT GTATGGTAGT
    TACAGTGGAA ACAGAGGCTG GTTTCCAGAG AGCTATGCTG AGAGATGCAG TAAAGACAGT
    CAGACTCAAC CGACCGCAGC TGAAACTGAG TCCACTGCGC CCTCTGCCAA CTACCCTGGA
    ATTCCTCGAG TGGACATAGA AGGACCAACA CCGACACACA CCCCAGTATC CACAAACACA
    CAGCACTCAC AGGCTGTTGC TCTGTGTGAG TGGAGCGCTA AGACTGACAG TCACCTGGGC
    TTCTGCAAGG ACGACATTAT TACAGTTCTG GAGAAGCAGG AGAACTGGTG GTACGGAGAA
    CTCAAGGAGA AACGGGGCTG GTTCCCCTGT TCACATGTCT CTGTGGTCAC CACCAATAAC
    ACACAGAGCG ATTTTGACAT GCCTGCTGGA AATCTTTTAA GTGTTTTGGT ATCTCTTTTA
    GGCCTCTGTA TTCTAGCGTG GAGGAGATTG AAGTGTCAGA CTCTGGTTTG CTTGATGTAT
    GTGGCTCTGT ACACGTATGA GAGTCCGGAG TCAGGAGACC TGACGTTCAG TGCAGAGGAT
    GTGGTTCTGG TTACAGAGAA AGAAGGAGAG TGGTGGAGAG GCTGTATTGG TGACCAGACC
    GGCCTTTTCC CCTCCAACTA TGTTAAACCC AAAGAACCTG ATACTGCCAG TTCTGCAGTT
    CCCAGTAAAA AGCCAGAAAT TGCTCAGGTG ACTACAGTCA GTCCTGCAAC AACACCAGAG
    CAGCTGAGTC TGACACCTGG CCAGCTGATC GTGGTGCTGC ATAAGAACTC AAACAGCTGG
    TGGCTTGGTG AACTACAGGC ACGGGGTAAG AAGCGTAAAA AGGGCTGGTT TCAATCTTCT
    AATGTCCGAT TACTGGAGGC CAACAGCGGC AAAATAACAC CTGCACCTCA ACCATTGTGT
    CAGGTTATTG CAATGTATGA CTACAAAGCA GCCAATAAGG ACGAGATGAG CTTCCAGAAA
    GGTCAGCTGA TCACCGTACT GAACAAGGAC AACCCTGACT GGTGGAAAGG AGAGGTCGCA
    GGGCTCACAG GGCTGTTTCC AACTAATTAT GTGAAGATGA CCACAGAATG TGATCCAAGC
    CAGCAATGGT GTGCTGACCT GCTTAGTTTA GACTCTATGT GCACTGAGGA GAGGAAGAGG
    CAAGGCTACA TCCATGAGCT CATTCAGACA GAAGAGTCTT ACCTGGAGGA CCTGGAGCTG
    GCAGTGGAGG TGTTTTATAA GCCAATGGCA GAGTCGGGAC GGCTGACTGA GGCTGAGATG
    AGCATGATCT TTGTGAACTG GCGTGAATTG ATCATGTGCA GCACCAAACT CCTAAAAGCT
    CTGCGTGTGC GTAAGAAGAC AGCAGGTGAG CGAATGCCCG TGCAGGTGGT TGGTGATATC
    TTGTCCTCTG AGCTCTCTCA CATGCAGGCC TACATTCGCT TCTGCAGCTG CCAGCTCAAC
    GCAGCCGCTC TGCTGCAGCA GAAAACAGAC AAATCACTCG ACTTCAAACT CTTCCTCAAG
    AAAATTGCCA GTAATTACCG CTGTAAAGGA ATGCCACTCT CCAGCTTTCT CCTGAAGCCC
    ATGCAGAGGA TCACACGATA CCCACTGCTG ATCAAAAACA TTCTGGAAAA CACCCCTCCG
    GCTCACGCTG ACCACTCCAA TCTGCAGGCG GCGCTGGAGC AGGCGGAGGA GCTTTGCTCA
    CAGGTGAACG AGGCCGTGAG GGAGAAAGAG AACTCCGACC GACTGGAGTG GATCCAGAAC
    CACGTGTTGT GCGACGGAGT CATTGAGCAC CTTGTTTTTA ACTCTCTGAC GAACTGCCTG
    GGCCCTCGAA AACTGCTGCA CAGTGGCAAA CTGCACAAGA CCAAGAGCAG CAAAGAGCTC
    TGGGCCCTGC TGTTCAATGA CTTCCTGCTC CTCACATACA CCTCCAAACA GTTCTCATCC
    AGCCCAGACA AACTCTTCAA TCCAAACTCC AACACACAGT ACAAGATGTA CAAGACGCCA
    GTGTTTCTGA ATGAGGTCTT GGTGAAAATG CCCTCTGACC CGTCCAGCGA TGATCCAGTT
    TTCCACATTT CACACATTGA TCGTGTTTAC ACACTAAAGA CTGACACTAT CAATGAGAGG
    ACGACGTGGG TGCAGAAGAT CAAAGCAGCA TCTGATCACT TTATAGAGAC GGAGAAGCTG
    AAGAGAGAGA AGGCGTATCA AGCTCGCTCT CAGAAGAACA GTGGCATCGG TCGACTGCTG
    GTAACAGTGT TGGAGGCCAC AGAGCTGAAG CCCTGCAAAC CCAACGGGAA GAGTAACCCA
    TACTGTGAAC TAACCATGGG CGCCCAGTGC TACACGTCCC GGCATCAGCC CGACACGCTC
    AACCCAAAAT GGAGCTTCAA CTGCCAGTTC TTCATCAAAG ACCTGTACCA GGATGTCCTG
    TGCCTCACTG TCTTTGAGAG GGACCAGTTC TCTCCTGATG ATTTTCTAGG TCGAACTGAA
    GTTCCTGTAG CGACAATAAA AAAAGATCAA GATGGTAAAG GGCCACTGGT TCGACGTCTG
    CTGCTTCATG AAGTCCCGAC TGGAGAGGTT AAAGTGAGGC TTGATCTGCA GCTCTATGAA
    CAAACACCTC ACGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150654
    Clone ID Related Accession (Same CDS sequence) XM_021468488.1 , XM_021468487.1
    Accession Version XM_021468488.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5001bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product intersectin 2a isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATGGTG GCTTTTCTAT TTGGGCTATA ACTCCAGAAG AGAGGGGGAA ACATGACAAA 
    CAGTTTGACT CGCTGGCTCC CACCCTGGGA TATTTGTCGG GAGATCAAGC AAGAAAGTTC
    TTCCTCCAAT CTGGTCTTCC CACATCGGTC CTGGCAGACA TCTGGGCTCT GGCGGACATA
    GGAAAGGATG GGAAGATGGA CAGATTGGAG TTCTCTATTG CAATGAAACT AATTAAACTG
    CAGCTGCAGG GTCAGCCACT TCCGTCCAGC CTGCCGATCA TCATGAAGCA GACGCCCACC
    CCTGTGATGA CGTCATCGTC ACGCTTCGGT ATGGGCTCCA TGCCAAATCT CTCCGCCATG
    TCGGTGATGC CCATCCTAAC ACCCATCCCC ACATGCCCTG TCATGGCCCC TTTAGTTCCT
    GTGCCCACCC TGATATCTGG GCCACTGCCT CAGATGCCCA ACTCTATCAG TGTGCCAGCC
    TTACCTAACG GCAATGCAAC TCTCCTCACA CCAACACCAG CATTCCCAGT CTCCAGTGGT
    TTGACTAAAT CTCACTCTCT CCTGGACCTT GGCTCTAGCA GTTCTAACTC GTCCTCCACC
    ACCTCCCTTG CGAGTAACTC CCCGAAAACG AGTAGTTCTG ATTGGGCAGT GCCTCAGTCA
    TCCCGTCTGA AGTACCGACA ACTGTTTAAC AGCCTGGACA AGCTAATGAG TGGATACCTG
    TCAGGTCCCC AGGTCAGAAA TGCCCTCACA GCTTCAAACC TCACACAAAC ACAGCTTGCC
    ACTATATGGT TTCTGGCTGA TGTGGATCGT GATGGGCAGT TGAGAGCAGA AGAGTTTATT
    TTAGCGATGC ATCTGGTTGA AATGGCCAAG ACAGGGCGGC CTTTACCTCT CACTTTACCT
    CCCGACCTTG TGCCTCCTTC ACTCAGAACA ACTAAGTCAT GTGACCTGCT AAACGGACCT
    GTTCCTCTGA TTAGCACTGA GCTGATTGAG CCAGAACTGC CACAGAAAAA CAAGAGCAAT
    GTTTCATATG AAGACAAACT GAGGGAAAAT TTCCAGAGGG GAAACGCAGA GCTGGAGAAG
    CGTCGCCTGG CTCTTCAGGA GCAGCAGAGG AGAGAGGAGG AGCGCAGGAG AGAAGAGGAG
    AGGCGAGAGG AGGAGAAGAG ACAGCAGAAG GAAAGAGAGG AGCGAGAGAG GAGAGAGAGA
    GAAGCGCGAG AAATGGAGAT CAGGAGACAG AGAGAGGAGG AGCGCAGGAT GGAGAGACAG
    AGAGAGCTGG AGCGACAGAG AGAGGAGGAA CGACTGAAAG AATTAGAGCG GCGAGAGGAA
    GCTGAGCGAC AAAGACGGAG GGAATGGGAG AAGAGCAAAC GTGTGGAGCT TCAGACTCAG
    AAAGAAAAGG AGCAGAATGA CATACAGAGA CTAAAGGACA GGAAGAGGAG TCTTGAGATT
    GAGCTGGAGG CCGTGGGTAA CAAGCATAAG CAGATCTCCA GTCAGCTGCG TGATGCTAAG
    AGTAAGAGAC AGGGGCAGTG GGCCGAGTTG GAGCTGATCA ACCAGAGGAG AGACGTCTGC
    ATTACTGATA TCAACTCACT GCAGTTACAG TTTGATGAGA TGCAGCGGCA GTTGAATCAG
    CTGGGCCCTG AAAAGCAGCG ACTGGCTGAC AGACTTGTAC ATTTTACCCA AAACATCTCC
    AGTCCTTTGA TAAACGATGT GAAACAGAGT GTGTGCGAGA AAGATCGGAG CTGTCGGAAG
    CTTAAGGAGC AGCTGGATGT TTTGGAGAGA GAAACCACGG CCAAACTCTC ACAAATGGAG
    CAGTACAACA AAGAAATCAA GTTTGTTGAA ATGGATGACT GTGTGCTACA AGGACTTCTC
    TCTTTGCTGG GCTGCTTAAC CAATCTCTTC CTTCTAATAA AGGAATTGAG ACAGAAGCAG
    AGCCAGCAGC AGGACGTCCT GGAGCAGCTC AGGAGGGTCA GATCTGACAA ACTCAAAGAG
    CTCCAGAGAC ACAGGAAAGA GGAAGAGGAG AGAAAGAGGA AGAAAGAAGA GGAGGCCGCC
    AGACAGGCCC AGTTGGAGCA GGAGCGGCTG GAGCAGGAGG AGGCTGAGCG GCAAAGGAAA
    CTTGCACAGG AACGAGAGGC CAAACTCAGG GAAGAGCAGC AGCGGCAGGC TCTGGCCAGA
    CTGCTGGAGG CTCAGGAGCA GGCCAGAGCC AAAGAGGAGA AGAGGAGAGC TGAGGAGAAG
    GAGAGGAATG AGCTAGATGT ACACACTCAA TCCAGTCTGC CTTTCAACAG CACAGAGAAC
    AACATTTTGC AGCCAGTAGT GATCCCTGAA GTCACGTGTA GTTCTCTGAC CACCTACAGA
    GCTCTCTATC CCTTCACTGC ACGCAACTCA GACGAGCTCA CGCTGGAGGC AGACTGTTTC
    ATCCAGGTGG ATGAATCGAC AGTCAGAGAG ACCGGCTGGC TGTATGGCAG TTACAGTGGA
    AACAGAGGCT GGTTTCCAGA GAGCTATGCT GAGAGATGCA GTAAAGACAG TCAGACTCAA
    CCGACCGCAG CTGAAACTGA GTCCACTGCG CCCTCTGCCA ACTACCCTGG AATTCCTCGA
    GTGGACATAG AAGGACCAAC ACCGACACAC ACCCCAGTAT CCACAAACAC ACAGCACTCA
    CAGGCTGTTG CTCTGTGTGA GTGGAGCGCT AAGACTGACA GTCACCTGGG CTTCTGCAAG
    GACGACATTA TTACAGTTCT GGAGAAGCAG GAGAACTGGT GGTACGGAGA ACTCAAGGAG
    AAACGGGGCT GGTTCCCCTG TTCACATGTC TCTGTGGTCA CCACCAATAA TACACAGAGC
    GAGCCTCTGT ATTCTAGCGT GGAGGAGATT GAAGTGTCAG ACTCTGGTTT GCTTGATGAG
    TATGTGGCTC TGTACACGTA TGAGAGTCCG GAGTCAGGAG ACCTGACGTT CAGTGCAGAG
    GATGTGGTTC TGGTTACAGA GAAAGAAGGA GAGTGGTGGA GAGGCTGTAT TGGTGACCAG
    ACCGGCCTTT TCCCCTCCAA CTATGTTAAA CCCAAAGAAC CTGATACTGC CAGTTCTGCA
    GTTCCCAGTA AAAAGCCAGA AATTGCTCAG GTGACTACAG TCAGTCCTGC AACAACACCA
    GAGCAGCTGA GTCTGACACC TGGCCAGCTG ATCGTGGTGC TGCATAAGAA CTCAAACAGC
    TGGTGGCTTG GTGAACTACA GGCACGGGGT AAGAAGCGTA AAAAGGGCTG GTTTCAATCT
    TCTAATGTCC GATTACTGGA GGCCAACAGC GGCAAAATAA CACCTGCACC TCAACCATTG
    TGTCAGGTTA TTGCAATGTA TGACTACAAA GCAGCCAATA AGGACGAGAT GAGCTTCCAG
    AAAGGTCAGC TGATCACCGT ACTGAACAAG GACAACCCTG ACTGGTGGAA AGGAGAGGTC
    GCAGGGGTCA CAGGGCTGTT TCCAACTAAT TATGTGAAGA TGACCACAGA ATGTGATCCA
    AGCCAGCAAT GGTGTGCTGA CCTGCTTAGT TTAGACTCTA TGTGCACTGA GGAGAGGAAG
    AGGCAAGGCT ACATCCATGA GCTCATTCAG ACAGAAGAGT CTTACCTGGA GGACCTGGAG
    CTGGCAGTGG AGGTGTTTTA TAAGCCAATG GCAGAGTCGG GACGGCTGAC CGAGGCTGAG
    ATCAGCATGA TCTTTGTGAA CTGGCGTGAA TTGATCATGT GCAGCACCAA ACTCCTAAAA
    GCTCTGCGTG TGCGTAAGAA GACAGCAGGT GAGCGAATGC CCGTGCAGGT GGTTGGTGAT
    ATCTTGTCCT CTGAGCTCTC TCACATGCAG GCTTACATTC GCTTCTGCAG CTGCCAGCTC
    AACGCAGCTG CTCTGTTGCA GCAGAAAACA GACAAATCAC TCGACTTCAA ACTCTTCCTC
    AAGAAAATTG CCAGTAATTA CCGCTGTAAA GGAATGCCAC TGTCCAGCTT TCTCCTGAAG
    CCCATGCAGA GGATCACACG ATACCCACTG CTGATCAAAA ACATTCTGGA AAACACTCCT
    CCGGCTCACG CTGACCACTC CAATCTGCAG GCGGCGCTGG AGCAGGCGGA GGAGCTTTGC
    TCACAGGTGA ACGAGGCTGT GAGGGAGAAA GAGAACTCCG ACCGACTGGA GTGGATCCAG
    AACCACGTGT TGTGTGACGG AGTCATTGAG CACCTTGTTT TTAACTCTCT GACGAACTGC
    CTGGGCCCTC GAAAACTGCT GCACAGCGGC AAACTGCATA AGACCAAGAG CAGCAAAGAG
    CTCTGGGCCC TGCTGTTCAA TGACTTCCTG CTCCTCACAT ACACCTCCAA ACAGTTCTCA
    TCCAGCCCAG ACAAACTCTT CAATCCCAAC TCCAACACAC AGTACAAGAT GTACAAGACG
    CCAGTGTTTC TGAATGAGGT CTTGGTGAAA ATGCCCTCTG ACCCGTCCAG CGATGACCCA
    GTTTTCCACA TTTCACACAT TGATCGTGTT TACACACTCA AGACTGACAC TATCAATGAG
    AGGACGACGT GGGTGCAGAA GATCAAAGCA GCATCTGATC ACTTTATAGA GACGGAGAAG
    CTGAAGAGAG AGAAGGCGTA TCAAGCTCGC TCTCAGAAGA ACAGTGGCAT CGGTCGACTG
    CTGGTAACAG TGCTGGAAGC CACAGAGCTG AAGCCCTGCA AACCCAACGG GAAGAGTAAC
    CCATACTGTG AACTAACCAT GGGCGCCCAG TGCTACACGT CCCGGCATCA GCCCGACACG
    CTCAACCCAA AATGGAGCTT CAACTGCCAG TTCTTCATCA AAGACTTGTA CCAGGATGTC
    CTGTGCCTCA CTGTCTTTGA GAGGGACCAG TTCTCTCCTG ATGATTTTCT AGGTCGAACT
    GAAGTTCCTG TAGCGACAAT AAAAAAAGAT CAAGATGGTA AAGGGCCACT GGTTCGACGT
    CTGCTGCTTC ATGAAGTCCC GACTGGAGAG GTTAAAGTGA GGCTTGATCT GCAGCTCTAT
    GAACAAACAC CTCACGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324163.1
    CDS115..5115
    Translation

    Target ORF information:

    RefSeq Version XM_021468488.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio intersectin 2a (itsn2a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468488.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATGGTG GCTTTTCTAT TTGGGCTATA ACTCCAGAAG AGAGGGGGAA ACATGACAAA 
    CAGTTTGACT CGCTGGCTCC CACCCTGGGA TATTTGTCGG GAGATCAAGC AAGAAAGTTC
    TTCCTCCAAT CTGGTCTTCC CACATCGGTC CTGGCAGACA TCTGGGCTCT GGCGGACATA
    GGAAAGGATG GGAAGATGGA CAGATTGGAG TTCTCTATTG CAATGAAACT AATTAAACTG
    CAGCTGCAGG GTCAGCCACT TCCGTCCAGC CTGCCGATCA TCATGAAGCA GACGCCCACC
    CCTGTGATGA CGTCATCGTC ACGCTTCGGT ATGGGCTCCA TGCCAAATCT CTCCGCCATG
    TCGGTGATGC CCATCCTAAC ACCCATCCCC ACATGCCCTG TCATGGCCCC TTTAGTTCCT
    GTGCCCACCC TGATATCTGG GCCACTGCCT CAGATGCCCA ACTCTATCAG TGTGCCAGCC
    TTACCTAACG GCAATGCAAC TCTCCTCACA CCAACACCAG CATTCCCAGT CTCCAGTGGT
    TTGACTAAAT CTCACTCTCT CCTGGACCTT GGCTCTAGCA GTTCTAACTC GTCCTCCACC
    ACCTCCCTTG CGAGTAACTC CCCGAAAACG AGTAGTTCTG ATTGGGCAGT GCCTCAGTCA
    TCCCGTCTGA AGTACCGACA ACTGTTTAAC AGCCTGGACA AGCTAATGAG TGGATACCTG
    TCAGGTCCCC AGGTCAGAAA TGCCCTCACA GCTTCAAACC TCACACAAAC ACAGCTTGCC
    ACTATATGGT TTCTGGCTGA TGTGGATCGT GATGGGCAGT TGAGAGCAGA AGAGTTTATT
    TTAGCGATGC ATCTGGTTGA AATGGCCAAG ACAGGGCGGC CTTTACCTCT CACTTTACCT
    CCCGACCTTG TGCCTCCTTC ACTCAGAACA ACTAAGTCAT GTGACCTGCT AAACGGACCT
    GTTCCTCTGA TTAGCACTGA GCTGATTGAG CCAGAACTGC CACAGAAAAA CAAGAGCAAT
    GTTTCATATG AAGACAAACT GAGGGAAAAT TTCCAGAGGG GAAACGCAGA GCTGGAGAAG
    CGTCGCCTGG CTCTTCAGGA GCAGCAGAGG AGAGAGGAGG AGCGCAGGAG AGAAGAGGAG
    AGGCGAGAGG AGGAGAAGAG ACAGCAGAAG GAAAGAGAGG AGCGAGAGAG GAGAGAGAGA
    GAAGCGCGAG AAATGGAGAT CAGGAGACAG AGAGAGGAGG AGCGCAGGAT GGAGAGACAG
    AGAGAGCTGG AGCGACAGAG AGAGGAGGAA CGACTGAAAG AATTAGAGCG GCGAGAGGAA
    GCTGAGCGAC AAAGACGGAG GGAATGGGAG AAGAGCAAAC GTGTGGAGCT TCAGACTCAG
    AAAGAAAAGG AGCAGAATGA CATACAGAGA CTAAAGGACA GGAAGAGGAG TCTTGAGATT
    GAGCTGGAGG CCGTGGGTAA CAAGCATAAG CAGATCTCCA GTCAGCTGCG TGATGCTAAG
    AGTAAGAGAC AGGGGCAGTG GGCCGAGTTG GAGCTGATCA ACCAGAGGAG AGACGTCTGC
    ATTACTGATA TCAACTCACT GCAGTTACAG TTTGATGAGA TGCAGCGGCA GTTGAATCAG
    CTGGGCCCTG AAAAGCAGCG ACTGGCTGAC AGACTTGTAC ATTTTACCCA AAACATCTCC
    AGTCCTTTGA TAAACGATGT GAAACAGAGT GTGTGCGAGA AAGATCGGAG CTGTCGGAAG
    CTTAAGGAGC AGCTGGATGT TTTGGAGAGA GAAACCACGG CCAAACTCTC ACAAATGGAG
    CAGTACAACA AAGAAATCAA GTTTGTTGAA ATGGATGACT GTGTGCTACA AGGACTTCTC
    TCTTTGCTGG GCTGCTTAAC CAATCTCTTC CTTCTAATAA AGGAATTGAG ACAGAAGCAG
    AGCCAGCAGC AGGACGTCCT GGAGCAGCTC AGGAGGGTCA GATCTGACAA ACTCAAAGAG
    CTCCAGAGAC ACAGGAAAGA GGAAGAGGAG AGAAAGAGGA AGAAAGAAGA GGAGGCCGCC
    AGACAGGCCC AGTTGGAGCA GGAGCGGCTG GAGCAGGAGG AGGCTGAGCG GCAAAGGAAA
    CTTGCACAGG AACGAGAGGC CAAACTCAGG GAAGAGCAGC AGCGGCAGGC TCTGGCCAGA
    CTGCTGGAGG CTCAGGAGCA GGCCAGAGCC AAAGAGGAGA AGAGGAGAGC TGAGGAGAAG
    GAGAGGAATG AGCTAGATGT ACACACTCAA TCCAGTCTGC CTTTCAACAG CACAGAGAAC
    AACATTTTGC AGCCAGTAGT GATCCCTGAA GTCACGTGTA GTTCTCTGAC CACCTACAGA
    GCTCTCTATC CCTTCACTGC ACGCAACTCA GACGAGCTCA CGCTGGAGGC AGACTGTTTC
    ATCCAGGTGG ATGAATCGAC AGTCAGAGAG ACCGGCTGGC TGTATGGCAG TTACAGTGGA
    AACAGAGGCT GGTTTCCAGA GAGCTATGCT GAGAGATGCA GTAAAGACAG TCAGACTCAA
    CCGACCGCAG CTGAAACTGA GTCCACTGCG CCCTCTGCCA ACTACCCTGG AATTCCTCGA
    GTGGACATAG AAGGACCAAC ACCGACACAC ACCCCAGTAT CCACAAACAC ACAGCACTCA
    CAGGCTGTTG CTCTGTGTGA GTGGAGCGCT AAGACTGACA GTCACCTGGG CTTCTGCAAG
    GACGACATTA TTACAGTTCT GGAGAAGCAG GAGAACTGGT GGTACGGAGA ACTCAAGGAG
    AAACGGGGCT GGTTCCCCTG TTCACATGTC TCTGTGGTCA CCACCAATAA TACACAGAGC
    GAGCCTCTGT ATTCTAGCGT GGAGGAGATT GAAGTGTCAG ACTCTGGTTT GCTTGATGAG
    TATGTGGCTC TGTACACGTA TGAGAGTCCG GAGTCAGGAG ACCTGACGTT CAGTGCAGAG
    GATGTGGTTC TGGTTACAGA GAAAGAAGGA GAGTGGTGGA GAGGCTGTAT TGGTGACCAG
    ACCGGCCTTT TCCCCTCCAA CTATGTTAAA CCCAAAGAAC CTGATACTGC CAGTTCTGCA
    GTTCCCAGTA AAAAGCCAGA AATTGCTCAG GTGACTACAG TCAGTCCTGC AACAACACCA
    GAGCAGCTGA GTCTGACACC TGGCCAGCTG ATCGTGGTGC TGCATAAGAA CTCAAACAGC
    TGGTGGCTTG GTGAACTACA GGCACGGGGT AAGAAGCGTA AAAAGGGCTG GTTTCAATCT
    TCTAATGTCC GATTACTGGA GGCCAACAGC GGCAAAATAA CACCTGCACC TCAACCATTG
    TGTCAGGTTA TTGCAATGTA TGACTACAAA GCAGCCAATA AGGACGAGAT GAGCTTCCAG
    AAAGGTCAGC TGATCACCGT ACTGAACAAG GACAACCCTG ACTGGTGGAA AGGAGAGGTC
    GCAGGGGTCA CAGGGCTGTT TCCAACTAAT TATGTGAAGA TGACCACAGA ATGTGATCCA
    AGCCAGCAAT GGTGTGCTGA CCTGCTTAGT TTAGACTCTA TGTGCACTGA GGAGAGGAAG
    AGGCAAGGCT ACATCCATGA GCTCATTCAG ACAGAAGAGT CTTACCTGGA GGACCTGGAG
    CTGGCAGTGG AGGTGTTTTA TAAGCCAATG GCAGAGTCGG GACGGCTGAC CGAGGCTGAG
    ATCAGCATGA TCTTTGTGAA CTGGCGTGAA TTGATCATGT GCAGCACCAA ACTCCTAAAA
    GCTCTGCGTG TGCGTAAGAA GACAGCAGGT GAGCGAATGC CCGTGCAGGT GGTTGGTGAT
    ATCTTGTCCT CTGAGCTCTC TCACATGCAG GCTTACATTC GCTTCTGCAG CTGCCAGCTC
    AACGCAGCTG CTCTGTTGCA GCAGAAAACA GACAAATCAC TCGACTTCAA ACTCTTCCTC
    AAGAAAATTG CCAGTAATTA CCGCTGTAAA GGAATGCCAC TGTCCAGCTT TCTCCTGAAG
    CCCATGCAGA GGATCACACG ATACCCACTG CTGATCAAAA ACATTCTGGA AAACACTCCT
    CCGGCTCACG CTGACCACTC CAATCTGCAG GCGGCGCTGG AGCAGGCGGA GGAGCTTTGC
    TCACAGGTGA ACGAGGCTGT GAGGGAGAAA GAGAACTCCG ACCGACTGGA GTGGATCCAG
    AACCACGTGT TGTGTGACGG AGTCATTGAG CACCTTGTTT TTAACTCTCT GACGAACTGC
    CTGGGCCCTC GAAAACTGCT GCACAGCGGC AAACTGCATA AGACCAAGAG CAGCAAAGAG
    CTCTGGGCCC TGCTGTTCAA TGACTTCCTG CTCCTCACAT ACACCTCCAA ACAGTTCTCA
    TCCAGCCCAG ACAAACTCTT CAATCCCAAC TCCAACACAC AGTACAAGAT GTACAAGACG
    CCAGTGTTTC TGAATGAGGT CTTGGTGAAA ATGCCCTCTG ACCCGTCCAG CGATGACCCA
    GTTTTCCACA TTTCACACAT TGATCGTGTT TACACACTCA AGACTGACAC TATCAATGAG
    AGGACGACGT GGGTGCAGAA GATCAAAGCA GCATCTGATC ACTTTATAGA GACGGAGAAG
    CTGAAGAGAG AGAAGGCGTA TCAAGCTCGC TCTCAGAAGA ACAGTGGCAT CGGTCGACTG
    CTGGTAACAG TGCTGGAAGC CACAGAGCTG AAGCCCTGCA AACCCAACGG GAAGAGTAAC
    CCATACTGTG AACTAACCAT GGGCGCCCAG TGCTACACGT CCCGGCATCA GCCCGACACG
    CTCAACCCAA AATGGAGCTT CAACTGCCAG TTCTTCATCA AAGACTTGTA CCAGGATGTC
    CTGTGCCTCA CTGTCTTTGA GAGGGACCAG TTCTCTCCTG ATGATTTTCT AGGTCGAACT
    GAAGTTCCTG TAGCGACAAT AAAAAAAGAT CAAGATGGTA AAGGGCCACT GGTTCGACGT
    CTGCTGCTTC ATGAAGTCCC GACTGGAGAG GTTAAAGTGA GGCTTGATCT GCAGCTCTAT
    GAACAAACAC CTCACGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150654
    Clone ID Related Accession (Same CDS sequence) XM_021468488.1 , XM_021468487.1
    Accession Version XM_021468487.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5001bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product intersectin 2a isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATGGTG GCTTTTCTAT TTGGGCTATA ACTCCAGAAG AGAGGGGGAA ACATGACAAA 
    CAGTTTGACT CGCTGGCTCC CACCCTGGGA TATTTGTCGG GAGATCAAGC AAGAAAGTTC
    TTCCTCCAAT CTGGTCTTCC CACATCGGTC CTGGCAGACA TCTGGGCTCT GGCGGACATA
    GGAAAGGATG GGAAGATGGA CAGATTGGAG TTCTCTATTG CAATGAAACT AATTAAACTG
    CAGCTGCAGG GTCAGCCACT TCCGTCCAGC CTGCCGATCA TCATGAAGCA GACGCCCACC
    CCTGTGATGA CGTCATCGTC ACGCTTCGGT ATGGGCTCCA TGCCAAATCT CTCCGCCATG
    TCGGTGATGC CCATCCTAAC ACCCATCCCC ACATGCCCTG TCATGGCCCC TTTAGTTCCT
    GTGCCCACCC TGATATCTGG GCCACTGCCT CAGATGCCCA ACTCTATCAG TGTGCCAGCC
    TTACCTAACG GCAATGCAAC TCTCCTCACA CCAACACCAG CATTCCCAGT CTCCAGTGGT
    TTGACTAAAT CTCACTCTCT CCTGGACCTT GGCTCTAGCA GTTCTAACTC GTCCTCCACC
    ACCTCCCTTG CGAGTAACTC CCCGAAAACG AGTAGTTCTG ATTGGGCAGT GCCTCAGTCA
    TCCCGTCTGA AGTACCGACA ACTGTTTAAC AGCCTGGACA AGCTAATGAG TGGATACCTG
    TCAGGTCCCC AGGTCAGAAA TGCCCTCACA GCTTCAAACC TCACACAAAC ACAGCTTGCC
    ACTATATGGT TTCTGGCTGA TGTGGATCGT GATGGGCAGT TGAGAGCAGA AGAGTTTATT
    TTAGCGATGC ATCTGGTTGA AATGGCCAAG ACAGGGCGGC CTTTACCTCT CACTTTACCT
    CCCGACCTTG TGCCTCCTTC ACTCAGAACA ACTAAGTCAT GTGACCTGCT AAACGGACCT
    GTTCCTCTGA TTAGCACTGA GCTGATTGAG CCAGAACTGC CACAGAAAAA CAAGAGCAAT
    GTTTCATATG AAGACAAACT GAGGGAAAAT TTCCAGAGGG GAAACGCAGA GCTGGAGAAG
    CGTCGCCTGG CTCTTCAGGA GCAGCAGAGG AGAGAGGAGG AGCGCAGGAG AGAAGAGGAG
    AGGCGAGAGG AGGAGAAGAG ACAGCAGAAG GAAAGAGAGG AGCGAGAGAG GAGAGAGAGA
    GAAGCGCGAG AAATGGAGAT CAGGAGACAG AGAGAGGAGG AGCGCAGGAT GGAGAGACAG
    AGAGAGCTGG AGCGACAGAG AGAGGAGGAA CGACTGAAAG AATTAGAGCG GCGAGAGGAA
    GCTGAGCGAC AAAGACGGAG GGAATGGGAG AAGAGCAAAC GTGTGGAGCT TCAGACTCAG
    AAAGAAAAGG AGCAGAATGA CATACAGAGA CTAAAGGACA GGAAGAGGAG TCTTGAGATT
    GAGCTGGAGG CCGTGGGTAA CAAGCATAAG CAGATCTCCA GTCAGCTGCG TGATGCTAAG
    AGTAAGAGAC AGGGGCAGTG GGCCGAGTTG GAGCTGATCA ACCAGAGGAG AGACGTCTGC
    ATTACTGATA TCAACTCACT GCAGTTACAG TTTGATGAGA TGCAGCGGCA GTTGAATCAG
    CTGGGCCCTG AAAAGCAGCG ACTGGCTGAC AGACTTGTAC ATTTTACCCA AAACATCTCC
    AGTCCTTTGA TAAACGATGT GAAACAGAGT GTGTGCGAGA AAGATCGGAG CTGTCGGAAG
    CTTAAGGAGC AGCTGGATGT TTTGGAGAGA GAAACCACGG CCAAACTCTC ACAAATGGAG
    CAGTACAACA AAGAAATCAA GTTTGTTGAA ATGGATGACT GTGTGCTACA AGGACTTCTC
    TCTTTGCTGG GCTGCTTAAC CAATCTCTTC CTTCTAATAA AGGAATTGAG ACAGAAGCAG
    AGCCAGCAGC AGGACGTCCT GGAGCAGCTC AGGAGGGTCA GATCTGACAA ACTCAAAGAG
    CTCCAGAGAC ACAGGAAAGA GGAAGAGGAG AGAAAGAGGA AGAAAGAAGA GGAGGCCGCC
    AGACAGGCCC AGTTGGAGCA GGAGCGGCTG GAGCAGGAGG AGGCTGAGCG GCAAAGGAAA
    CTTGCACAGG AACGAGAGGC CAAACTCAGG GAAGAGCAGC AGCGGCAGGC TCTGGCCAGA
    CTGCTGGAGG CTCAGGAGCA GGCCAGAGCC AAAGAGGAGA AGAGGAGAGC TGAGGAGAAG
    GAGAGGAATG AGCTAGATGT ACACACTCAA TCCAGTCTGC CTTTCAACAG CACAGAGAAC
    AACATTTTGC AGCCAGTAGT GATCCCTGAA GTCACGTGTA GTTCTCTGAC CACCTACAGA
    GCTCTCTATC CCTTCACTGC ACGCAACTCA GACGAGCTCA CGCTGGAGGC AGACTGTTTC
    ATCCAGGTGG ATGAATCGAC AGTCAGAGAG ACCGGCTGGC TGTATGGCAG TTACAGTGGA
    AACAGAGGCT GGTTTCCAGA GAGCTATGCT GAGAGATGCA GTAAAGACAG TCAGACTCAA
    CCGACCGCAG CTGAAACTGA GTCCACTGCG CCCTCTGCCA ACTACCCTGG AATTCCTCGA
    GTGGACATAG AAGGACCAAC ACCGACACAC ACCCCAGTAT CCACAAACAC ACAGCACTCA
    CAGGCTGTTG CTCTGTGTGA GTGGAGCGCT AAGACTGACA GTCACCTGGG CTTCTGCAAG
    GACGACATTA TTACAGTTCT GGAGAAGCAG GAGAACTGGT GGTACGGAGA ACTCAAGGAG
    AAACGGGGCT GGTTCCCCTG TTCACATGTC TCTGTGGTCA CCACCAATAA TACACAGAGC
    GAGCCTCTGT ATTCTAGCGT GGAGGAGATT GAAGTGTCAG ACTCTGGTTT GCTTGATGAG
    TATGTGGCTC TGTACACGTA TGAGAGTCCG GAGTCAGGAG ACCTGACGTT CAGTGCAGAG
    GATGTGGTTC TGGTTACAGA GAAAGAAGGA GAGTGGTGGA GAGGCTGTAT TGGTGACCAG
    ACCGGCCTTT TCCCCTCCAA CTATGTTAAA CCCAAAGAAC CTGATACTGC CAGTTCTGCA
    GTTCCCAGTA AAAAGCCAGA AATTGCTCAG GTGACTACAG TCAGTCCTGC AACAACACCA
    GAGCAGCTGA GTCTGACACC TGGCCAGCTG ATCGTGGTGC TGCATAAGAA CTCAAACAGC
    TGGTGGCTTG GTGAACTACA GGCACGGGGT AAGAAGCGTA AAAAGGGCTG GTTTCAATCT
    TCTAATGTCC GATTACTGGA GGCCAACAGC GGCAAAATAA CACCTGCACC TCAACCATTG
    TGTCAGGTTA TTGCAATGTA TGACTACAAA GCAGCCAATA AGGACGAGAT GAGCTTCCAG
    AAAGGTCAGC TGATCACCGT ACTGAACAAG GACAACCCTG ACTGGTGGAA AGGAGAGGTC
    GCAGGGGTCA CAGGGCTGTT TCCAACTAAT TATGTGAAGA TGACCACAGA ATGTGATCCA
    AGCCAGCAAT GGTGTGCTGA CCTGCTTAGT TTAGACTCTA TGTGCACTGA GGAGAGGAAG
    AGGCAAGGCT ACATCCATGA GCTCATTCAG ACAGAAGAGT CTTACCTGGA GGACCTGGAG
    CTGGCAGTGG AGGTGTTTTA TAAGCCAATG GCAGAGTCGG GACGGCTGAC CGAGGCTGAG
    ATCAGCATGA TCTTTGTGAA CTGGCGTGAA TTGATCATGT GCAGCACCAA ACTCCTAAAA
    GCTCTGCGTG TGCGTAAGAA GACAGCAGGT GAGCGAATGC CCGTGCAGGT GGTTGGTGAT
    ATCTTGTCCT CTGAGCTCTC TCACATGCAG GCTTACATTC GCTTCTGCAG CTGCCAGCTC
    AACGCAGCTG CTCTGTTGCA GCAGAAAACA GACAAATCAC TCGACTTCAA ACTCTTCCTC
    AAGAAAATTG CCAGTAATTA CCGCTGTAAA GGAATGCCAC TGTCCAGCTT TCTCCTGAAG
    CCCATGCAGA GGATCACACG ATACCCACTG CTGATCAAAA ACATTCTGGA AAACACTCCT
    CCGGCTCACG CTGACCACTC CAATCTGCAG GCGGCGCTGG AGCAGGCGGA GGAGCTTTGC
    TCACAGGTGA ACGAGGCTGT GAGGGAGAAA GAGAACTCCG ACCGACTGGA GTGGATCCAG
    AACCACGTGT TGTGTGACGG AGTCATTGAG CACCTTGTTT TTAACTCTCT GACGAACTGC
    CTGGGCCCTC GAAAACTGCT GCACAGCGGC AAACTGCATA AGACCAAGAG CAGCAAAGAG
    CTCTGGGCCC TGCTGTTCAA TGACTTCCTG CTCCTCACAT ACACCTCCAA ACAGTTCTCA
    TCCAGCCCAG ACAAACTCTT CAATCCCAAC TCCAACACAC AGTACAAGAT GTACAAGACG
    CCAGTGTTTC TGAATGAGGT CTTGGTGAAA ATGCCCTCTG ACCCGTCCAG CGATGACCCA
    GTTTTCCACA TTTCACACAT TGATCGTGTT TACACACTCA AGACTGACAC TATCAATGAG
    AGGACGACGT GGGTGCAGAA GATCAAAGCA GCATCTGATC ACTTTATAGA GACGGAGAAG
    CTGAAGAGAG AGAAGGCGTA TCAAGCTCGC TCTCAGAAGA ACAGTGGCAT CGGTCGACTG
    CTGGTAACAG TGCTGGAAGC CACAGAGCTG AAGCCCTGCA AACCCAACGG GAAGAGTAAC
    CCATACTGTG AACTAACCAT GGGCGCCCAG TGCTACACGT CCCGGCATCA GCCCGACACG
    CTCAACCCAA AATGGAGCTT CAACTGCCAG TTCTTCATCA AAGACTTGTA CCAGGATGTC
    CTGTGCCTCA CTGTCTTTGA GAGGGACCAG TTCTCTCCTG ATGATTTTCT AGGTCGAACT
    GAAGTTCCTG TAGCGACAAT AAAAAAAGAT CAAGATGGTA AAGGGCCACT GGTTCGACGT
    CTGCTGCTTC ATGAAGTCCC GACTGGAGAG GTTAAAGTGA GGCTTGATCT GCAGCTCTAT
    GAACAAACAC CTCACGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324162.1
    CDS329..5329
    Translation

    Target ORF information:

    RefSeq Version XM_021468487.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio intersectin 2a (itsn2a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468487.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGAATGGTG GCTTTTCTAT TTGGGCTATA ACTCCAGAAG AGAGGGGGAA ACATGACAAA 
    CAGTTTGACT CGCTGGCTCC CACCCTGGGA TATTTGTCGG GAGATCAAGC AAGAAAGTTC
    TTCCTCCAAT CTGGTCTTCC CACATCGGTC CTGGCAGACA TCTGGGCTCT GGCGGACATA
    GGAAAGGATG GGAAGATGGA CAGATTGGAG TTCTCTATTG CAATGAAACT AATTAAACTG
    CAGCTGCAGG GTCAGCCACT TCCGTCCAGC CTGCCGATCA TCATGAAGCA GACGCCCACC
    CCTGTGATGA CGTCATCGTC ACGCTTCGGT ATGGGCTCCA TGCCAAATCT CTCCGCCATG
    TCGGTGATGC CCATCCTAAC ACCCATCCCC ACATGCCCTG TCATGGCCCC TTTAGTTCCT
    GTGCCCACCC TGATATCTGG GCCACTGCCT CAGATGCCCA ACTCTATCAG TGTGCCAGCC
    TTACCTAACG GCAATGCAAC TCTCCTCACA CCAACACCAG CATTCCCAGT CTCCAGTGGT
    TTGACTAAAT CTCACTCTCT CCTGGACCTT GGCTCTAGCA GTTCTAACTC GTCCTCCACC
    ACCTCCCTTG CGAGTAACTC CCCGAAAACG AGTAGTTCTG ATTGGGCAGT GCCTCAGTCA
    TCCCGTCTGA AGTACCGACA ACTGTTTAAC AGCCTGGACA AGCTAATGAG TGGATACCTG
    TCAGGTCCCC AGGTCAGAAA TGCCCTCACA GCTTCAAACC TCACACAAAC ACAGCTTGCC
    ACTATATGGT TTCTGGCTGA TGTGGATCGT GATGGGCAGT TGAGAGCAGA AGAGTTTATT
    TTAGCGATGC ATCTGGTTGA AATGGCCAAG ACAGGGCGGC CTTTACCTCT CACTTTACCT
    CCCGACCTTG TGCCTCCTTC ACTCAGAACA ACTAAGTCAT GTGACCTGCT AAACGGACCT
    GTTCCTCTGA TTAGCACTGA GCTGATTGAG CCAGAACTGC CACAGAAAAA CAAGAGCAAT
    GTTTCATATG AAGACAAACT GAGGGAAAAT TTCCAGAGGG GAAACGCAGA GCTGGAGAAG
    CGTCGCCTGG CTCTTCAGGA GCAGCAGAGG AGAGAGGAGG AGCGCAGGAG AGAAGAGGAG
    AGGCGAGAGG AGGAGAAGAG ACAGCAGAAG GAAAGAGAGG AGCGAGAGAG GAGAGAGAGA
    GAAGCGCGAG AAATGGAGAT CAGGAGACAG AGAGAGGAGG AGCGCAGGAT GGAGAGACAG
    AGAGAGCTGG AGCGACAGAG AGAGGAGGAA CGACTGAAAG AATTAGAGCG GCGAGAGGAA
    GCTGAGCGAC AAAGACGGAG GGAATGGGAG AAGAGCAAAC GTGTGGAGCT TCAGACTCAG
    AAAGAAAAGG AGCAGAATGA CATACAGAGA CTAAAGGACA GGAAGAGGAG TCTTGAGATT
    GAGCTGGAGG CCGTGGGTAA CAAGCATAAG CAGATCTCCA GTCAGCTGCG TGATGCTAAG
    AGTAAGAGAC AGGGGCAGTG GGCCGAGTTG GAGCTGATCA ACCAGAGGAG AGACGTCTGC
    ATTACTGATA TCAACTCACT GCAGTTACAG TTTGATGAGA TGCAGCGGCA GTTGAATCAG
    CTGGGCCCTG AAAAGCAGCG ACTGGCTGAC AGACTTGTAC ATTTTACCCA AAACATCTCC
    AGTCCTTTGA TAAACGATGT GAAACAGAGT GTGTGCGAGA AAGATCGGAG CTGTCGGAAG
    CTTAAGGAGC AGCTGGATGT TTTGGAGAGA GAAACCACGG CCAAACTCTC ACAAATGGAG
    CAGTACAACA AAGAAATCAA GTTTGTTGAA ATGGATGACT GTGTGCTACA AGGACTTCTC
    TCTTTGCTGG GCTGCTTAAC CAATCTCTTC CTTCTAATAA AGGAATTGAG ACAGAAGCAG
    AGCCAGCAGC AGGACGTCCT GGAGCAGCTC AGGAGGGTCA GATCTGACAA ACTCAAAGAG
    CTCCAGAGAC ACAGGAAAGA GGAAGAGGAG AGAAAGAGGA AGAAAGAAGA GGAGGCCGCC
    AGACAGGCCC AGTTGGAGCA GGAGCGGCTG GAGCAGGAGG AGGCTGAGCG GCAAAGGAAA
    CTTGCACAGG AACGAGAGGC CAAACTCAGG GAAGAGCAGC AGCGGCAGGC TCTGGCCAGA
    CTGCTGGAGG CTCAGGAGCA GGCCAGAGCC AAAGAGGAGA AGAGGAGAGC TGAGGAGAAG
    GAGAGGAATG AGCTAGATGT ACACACTCAA TCCAGTCTGC CTTTCAACAG CACAGAGAAC
    AACATTTTGC AGCCAGTAGT GATCCCTGAA GTCACGTGTA GTTCTCTGAC CACCTACAGA
    GCTCTCTATC CCTTCACTGC ACGCAACTCA GACGAGCTCA CGCTGGAGGC AGACTGTTTC
    ATCCAGGTGG ATGAATCGAC AGTCAGAGAG ACCGGCTGGC TGTATGGCAG TTACAGTGGA
    AACAGAGGCT GGTTTCCAGA GAGCTATGCT GAGAGATGCA GTAAAGACAG TCAGACTCAA
    CCGACCGCAG CTGAAACTGA GTCCACTGCG CCCTCTGCCA ACTACCCTGG AATTCCTCGA
    GTGGACATAG AAGGACCAAC ACCGACACAC ACCCCAGTAT CCACAAACAC ACAGCACTCA
    CAGGCTGTTG CTCTGTGTGA GTGGAGCGCT AAGACTGACA GTCACCTGGG CTTCTGCAAG
    GACGACATTA TTACAGTTCT GGAGAAGCAG GAGAACTGGT GGTACGGAGA ACTCAAGGAG
    AAACGGGGCT GGTTCCCCTG TTCACATGTC TCTGTGGTCA CCACCAATAA TACACAGAGC
    GAGCCTCTGT ATTCTAGCGT GGAGGAGATT GAAGTGTCAG ACTCTGGTTT GCTTGATGAG
    TATGTGGCTC TGTACACGTA TGAGAGTCCG GAGTCAGGAG ACCTGACGTT CAGTGCAGAG
    GATGTGGTTC TGGTTACAGA GAAAGAAGGA GAGTGGTGGA GAGGCTGTAT TGGTGACCAG
    ACCGGCCTTT TCCCCTCCAA CTATGTTAAA CCCAAAGAAC CTGATACTGC CAGTTCTGCA
    GTTCCCAGTA AAAAGCCAGA AATTGCTCAG GTGACTACAG TCAGTCCTGC AACAACACCA
    GAGCAGCTGA GTCTGACACC TGGCCAGCTG ATCGTGGTGC TGCATAAGAA CTCAAACAGC
    TGGTGGCTTG GTGAACTACA GGCACGGGGT AAGAAGCGTA AAAAGGGCTG GTTTCAATCT
    TCTAATGTCC GATTACTGGA GGCCAACAGC GGCAAAATAA CACCTGCACC TCAACCATTG
    TGTCAGGTTA TTGCAATGTA TGACTACAAA GCAGCCAATA AGGACGAGAT GAGCTTCCAG
    AAAGGTCAGC TGATCACCGT ACTGAACAAG GACAACCCTG ACTGGTGGAA AGGAGAGGTC
    GCAGGGGTCA CAGGGCTGTT TCCAACTAAT TATGTGAAGA TGACCACAGA ATGTGATCCA
    AGCCAGCAAT GGTGTGCTGA CCTGCTTAGT TTAGACTCTA TGTGCACTGA GGAGAGGAAG
    AGGCAAGGCT ACATCCATGA GCTCATTCAG ACAGAAGAGT CTTACCTGGA GGACCTGGAG
    CTGGCAGTGG AGGTGTTTTA TAAGCCAATG GCAGAGTCGG GACGGCTGAC CGAGGCTGAG
    ATCAGCATGA TCTTTGTGAA CTGGCGTGAA TTGATCATGT GCAGCACCAA ACTCCTAAAA
    GCTCTGCGTG TGCGTAAGAA GACAGCAGGT GAGCGAATGC CCGTGCAGGT GGTTGGTGAT
    ATCTTGTCCT CTGAGCTCTC TCACATGCAG GCTTACATTC GCTTCTGCAG CTGCCAGCTC
    AACGCAGCTG CTCTGTTGCA GCAGAAAACA GACAAATCAC TCGACTTCAA ACTCTTCCTC
    AAGAAAATTG CCAGTAATTA CCGCTGTAAA GGAATGCCAC TGTCCAGCTT TCTCCTGAAG
    CCCATGCAGA GGATCACACG ATACCCACTG CTGATCAAAA ACATTCTGGA AAACACTCCT
    CCGGCTCACG CTGACCACTC CAATCTGCAG GCGGCGCTGG AGCAGGCGGA GGAGCTTTGC
    TCACAGGTGA ACGAGGCTGT GAGGGAGAAA GAGAACTCCG ACCGACTGGA GTGGATCCAG
    AACCACGTGT TGTGTGACGG AGTCATTGAG CACCTTGTTT TTAACTCTCT GACGAACTGC
    CTGGGCCCTC GAAAACTGCT GCACAGCGGC AAACTGCATA AGACCAAGAG CAGCAAAGAG
    CTCTGGGCCC TGCTGTTCAA TGACTTCCTG CTCCTCACAT ACACCTCCAA ACAGTTCTCA
    TCCAGCCCAG ACAAACTCTT CAATCCCAAC TCCAACACAC AGTACAAGAT GTACAAGACG
    CCAGTGTTTC TGAATGAGGT CTTGGTGAAA ATGCCCTCTG ACCCGTCCAG CGATGACCCA
    GTTTTCCACA TTTCACACAT TGATCGTGTT TACACACTCA AGACTGACAC TATCAATGAG
    AGGACGACGT GGGTGCAGAA GATCAAAGCA GCATCTGATC ACTTTATAGA GACGGAGAAG
    CTGAAGAGAG AGAAGGCGTA TCAAGCTCGC TCTCAGAAGA ACAGTGGCAT CGGTCGACTG
    CTGGTAACAG TGCTGGAAGC CACAGAGCTG AAGCCCTGCA AACCCAACGG GAAGAGTAAC
    CCATACTGTG AACTAACCAT GGGCGCCCAG TGCTACACGT CCCGGCATCA GCCCGACACG
    CTCAACCCAA AATGGAGCTT CAACTGCCAG TTCTTCATCA AAGACTTGTA CCAGGATGTC
    CTGTGCCTCA CTGTCTTTGA GAGGGACCAG TTCTCTCCTG ATGATTTTCT AGGTCGAACT
    GAAGTTCCTG TAGCGACAAT AAAAAAAGAT CAAGATGGTA AAGGGCCACT GGTTCGACGT
    CTGCTGCTTC ATGAAGTCCC GACTGGAGAG GTTAAAGTGA GGCTTGATCT GCAGCTCTAT
    GAACAAACAC CTCACGTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa150655
    Clone ID Related Accession (Same CDS sequence) XM_021468489.1
    Accession Version XM_021468489.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3492bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product intersectin 2a isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAATGGTG GCTTTTCTAT TTGGGCTATA ACTCCAGAAG AGAGGGGGAA ACATGACAAA 
    CAGTTTGACT CGCTGGCTCC CACCCTGGGA TATTTGTCGG GAGATCAAGC AAGAAAGTTC
    TTCCTCCAAT CTGGTCTTCC CACATCGGTC CTGGCAGACA TCTGGGCTCT GGCGGACATA
    GGAAAGGATG GGAAGATGGA CAGATTGGAG TTCTCTATTG CAATGAAACT AATTAAACTG
    CAGCTGCAGG GTCAGCCACT TCCGTCCAGC CTGCCGATCA TCATGAAGCA GACGCCCACC
    CCTGTGATGA CGTCATCGTC ACGCTTCGGT ATGGGCTCCA TGCCAAATCT CTCCGCCATG
    TCGGTGATGC CCATCCTAAC ACCCATCCCC ACATGCCCTG TCATGGCCCC TTTAGTTCCT
    GTGCCCACCC TGATATCTGG GCCACTGCCT CAGATGCCCA ACTCTATCAG TGTGCCAGCC
    TTACCTAACG GCAATGCAAC TCTCCTCACA CCAACACCAG CATTCCCAGT CTCCAGTGGT
    TTGACTAAAT CTCACTCTCT CCTGGACCTT GGCTCTAGCA GTTCTAACTC GTCCTCCACC
    ACCTCCCTTG CGAGTAACTC CCCGAAAACG AGTAGTTCTG ATTGGGCAGT GCCTCAGTCA
    TCCCGTCTGA AGTACCGACA ACTGTTTAAC AGCCTGGACA AGCTAATGAG TGGATACCTG
    TCAGGTCCCC AGGTCAGAAA TGCCCTCACA GCTTCAAACC TCACACAAAC ACAGCTTGCC
    ACTATATGGT TTCTGGCTGA TGTGGATCGT GATGGGCAGT TGAGAGCAGA AGAGTTTATT
    TTAGCGATGC ATCTGGTTGA AATGGCCAAG ACAGGGCGGC CTTTACCTCT CACTTTACCT
    CCCGACCTTG TGCCTCCTTC ACTCAGAACA ACTAAGTCAT GTGACCTGCT AAACGGACCT
    GTTCCTCTGA TTAGCACTGA GCTGATTGAG CCAGAACTGC CACAGAAAAA CAAGAGCAAT
    GTTTCATATG AAGACAAACT GAGGGAAAAT TTCCAGAGGG GAAACGCAGA GCTGGAGAAG
    CGTCGCCTGG CTCTTCAGGA GCAGCAGAGG AGAGAGGAGG AGCGCAGGAG AGAAGAGGAG
    AGGCGAGAGG AGGAGAAGAG ACAGCAGAAG GAAAGAGAGG AGCGAGAGAG GAGAGAGAGA
    GAAGCGCGAG AAATGGAGAT CAGGAGACAG AGAGAGGAGG AGCGCAGGAT GGAGAGACAG
    AGAGAGCTGG AGCGACAGAG AGAGGAGGAA CGACTGAAAG AATTAGAGCG GCGAGAGGAA
    GCTGAGCGAC AAAGACGGAG GGAATGGGAG AAGAGCAAAC GTGTGGAGCT TCAGACTCAG
    AAAGAAAAGG AGCAGAATGA CATACAGAGA CTAAAGGACA GGAAGAGGAG TCTTGAGATT
    GAGCTGGAGG CCGTGGGTAA CAAGCATAAG CAGATCTCCA GTCAGCTGCG TGATGCTAAG
    AGTAAGAGAC AGGGGCAGTG GGCCGAGTTG GAGCTGATCA ACCAGAGGAG AGACGTCTGC
    ATTACTGATA TCAACTCACT GCAGTTACAG TTTGATGAGA TGCAGCGGCA GTTGAATCAG
    CTGGGCCCTG AAAAGCAGCG ACTGGCTGAC AGACTTGTAC ATTTTACCCA AAACATCTCC
    AGTCCTTTGA TAAACGATGT GAAACAGAGT GTGTGCGAGA AAGATCGGAG CTGTCGGAAG
    CTTAAGGAGC AGCTGGATGT TTTGGAGAGA GAAACCACGG CCAAACTCTC ACAAATGGAG
    CAGTACAACA AAGAAATCAA GTTTGTTGAA ATGGATGACT GTGTGCTACA AGGACTTCTC
    TCTTTGCTGG GCTGCTTAAC CAATCTCTTC CTTCTAATAA AGGAATTGAG ACAGAAGCAG
    AGCCAGCAGC AGGACGTCCT GGAGCAGCTC AGGAGGGTCA GATCTGACAA ACTCAAAGAG
    CTCCAGAGAC ACAGGAAAGA GGAAGAGGAG AGAAAGAGGA AGAAAGAAGA GGAGGCCGCC
    AGACAGGCCC AGTTGGAGCA GGAGCGGCTG GAGCAGGAGG AGGCTGAGCG GCAAAGGAAA
    CTTGCACAGG AACGAGAGGC CAAACTCAGG GAAGAGCAGC AGCGGCAGGC TCTGGCCAGA
    CTGCTGGAGG CTCAGGAGCA GGCCAGAGCC AAAGAGGAGA AGAGGAGAGC TGAGGAGAAG
    GAGAGGAATG AGCTAGATGT ACACACTCAA TCCAGTCTGC CTTTCAACAG CACAGAGAAC
    AACATTTTGC AGCCAGTAGT GATCCCTGAA GTCACGTGTA GTTCTCTGAC CACCTACAGA
    GCTCTCTATC CCTTCACTGC ACGCAACTCA GACGAGCTCA CGCTGGAGGC AGACTGTTTC
    ATCCAGGTGG ATGAATCGAC AGTCAGAGAG ACCGGCTGGC TGTATGGCAG TTACAGTGGA
    AACAGAGGCT GGTTTCCAGA GAGCTATGCT GAGAGATGCA GTAAAGACAG TCAGACTCAA
    CCGACCGCAG CTGAAACTGA GTCCACTGCG CCCTCTGCCA ACTACCCTGG AATTCCTCGA
    GTGGACATAG AAGGACCAAC ACCGACACAC ACCCCAGTAT CCACAAACAC ACAGCACTCA
    CAGGCTGTTG CTCTGTGTGA GTGGAGCGCT AAGACTGACA GTCACCTGGG CTTCTGCAAG
    GACGACATTA TTACAGTTCT GGAGAAGCAG GAGAACTGGT GGTACGGAGA ACTCAAGGAG
    AAACGGGGCT GGTTCCCCTG TTCACATGTC TCTGTGGTCA CCACCAATAA TACACAGAGC
    GAGCCTCTGT ATTCTAGCGT GGAGGAGATT GAAGTGTCAG ACTCTGGTTT GCTTGATGAG
    TATGTGGCTC TGTACACGTA TGAGAGTCCG GAGTCAGGAG ACCTGACGTT CAGTGCAGAG
    GATGTGGTTC TGGTTACAGA GAAAGAAGGA GAGTGGTGGA GAGGCTGTAT TGGTGACCAG
    ACCGGCCTTT TCCCCTCCAA CTATGTTAAA CCCAAAGAAC CTGATACTGC CAGTTCTGCA
    GTTCCCAGTA AAAAGCCAGA AATTGCTCAG GTGACTACAG TCAGTCCTGC AACAACACCA
    GAGCAGCTGA GTCTGACACC TGGCCAGCTG ATCGTGGTGC TGCATAAGAA CTCAAACAGC
    TGGTGGCTTG GTGAACTACA GGCACGGGGT AAGAAGCGTA AAAAGGGCTG GTTTCAATCT
    TCTAATGTCC GATTACTGGA GGCCAACAGC GGCAAAATAA CACCTGCACC TCAACCATTG
    TGTCAGGTTA TTGCAATGTA TGACTACAAA GCAGCCAATA AGGACGAGAT GAGCTTCCAG
    AAAGGTCAGC TGATCACCGT ACTGAACAAG GACAACCCTG ACTGGTGGAA AGGAGAGGTC
    GCAGGGGTCA CAGGGCTGTT TCCAACTAAT TATGTGAAGA TGACCACAGA ATGTGATCCA
    AGCCAGCAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021324164.1
    CDS333..3824
    Translation

    Target ORF information:

    RefSeq Version XM_021468489.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio intersectin 2a (itsn2a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021468489.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAATGGTG GCTTTTCTAT TTGGGCTATA ACTCCAGAAG AGAGGGGGAA ACATGACAAA 
    CAGTTTGACT CGCTGGCTCC CACCCTGGGA TATTTGTCGG GAGATCAAGC AAGAAAGTTC
    TTCCTCCAAT CTGGTCTTCC CACATCGGTC CTGGCAGACA TCTGGGCTCT GGCGGACATA
    GGAAAGGATG GGAAGATGGA CAGATTGGAG TTCTCTATTG CAATGAAACT AATTAAACTG
    CAGCTGCAGG GTCAGCCACT TCCGTCCAGC CTGCCGATCA TCATGAAGCA GACGCCCACC
    CCTGTGATGA CGTCATCGTC ACGCTTCGGT ATGGGCTCCA TGCCAAATCT CTCCGCCATG
    TCGGTGATGC CCATCCTAAC ACCCATCCCC ACATGCCCTG TCATGGCCCC TTTAGTTCCT
    GTGCCCACCC TGATATCTGG GCCACTGCCT CAGATGCCCA ACTCTATCAG TGTGCCAGCC
    TTACCTAACG GCAATGCAAC TCTCCTCACA CCAACACCAG CATTCCCAGT CTCCAGTGGT
    TTGACTAAAT CTCACTCTCT CCTGGACCTT GGCTCTAGCA GTTCTAACTC GTCCTCCACC
    ACCTCCCTTG CGAGTAACTC CCCGAAAACG AGTAGTTCTG ATTGGGCAGT GCCTCAGTCA
    TCCCGTCTGA AGTACCGACA ACTGTTTAAC AGCCTGGACA AGCTAATGAG TGGATACCTG
    TCAGGTCCCC AGGTCAGAAA TGCCCTCACA GCTTCAAACC TCACACAAAC ACAGCTTGCC
    ACTATATGGT TTCTGGCTGA TGTGGATCGT GATGGGCAGT TGAGAGCAGA AGAGTTTATT
    TTAGCGATGC ATCTGGTTGA AATGGCCAAG ACAGGGCGGC CTTTACCTCT CACTTTACCT
    CCCGACCTTG TGCCTCCTTC ACTCAGAACA ACTAAGTCAT GTGACCTGCT AAACGGACCT
    GTTCCTCTGA TTAGCACTGA GCTGATTGAG CCAGAACTGC CACAGAAAAA CAAGAGCAAT
    GTTTCATATG AAGACAAACT GAGGGAAAAT TTCCAGAGGG GAAACGCAGA GCTGGAGAAG
    CGTCGCCTGG CTCTTCAGGA GCAGCAGAGG AGAGAGGAGG AGCGCAGGAG AGAAGAGGAG
    AGGCGAGAGG AGGAGAAGAG ACAGCAGAAG GAAAGAGAGG AGCGAGAGAG GAGAGAGAGA
    GAAGCGCGAG AAATGGAGAT CAGGAGACAG AGAGAGGAGG AGCGCAGGAT GGAGAGACAG
    AGAGAGCTGG AGCGACAGAG AGAGGAGGAA CGACTGAAAG AATTAGAGCG GCGAGAGGAA
    GCTGAGCGAC AAAGACGGAG GGAATGGGAG AAGAGCAAAC GTGTGGAGCT TCAGACTCAG
    AAAGAAAAGG AGCAGAATGA CATACAGAGA CTAAAGGACA GGAAGAGGAG TCTTGAGATT
    GAGCTGGAGG CCGTGGGTAA CAAGCATAAG CAGATCTCCA GTCAGCTGCG TGATGCTAAG
    AGTAAGAGAC AGGGGCAGTG GGCCGAGTTG GAGCTGATCA ACCAGAGGAG AGACGTCTGC
    ATTACTGATA TCAACTCACT GCAGTTACAG TTTGATGAGA TGCAGCGGCA GTTGAATCAG
    CTGGGCCCTG AAAAGCAGCG ACTGGCTGAC AGACTTGTAC ATTTTACCCA AAACATCTCC
    AGTCCTTTGA TAAACGATGT GAAACAGAGT GTGTGCGAGA AAGATCGGAG CTGTCGGAAG
    CTTAAGGAGC AGCTGGATGT TTTGGAGAGA GAAACCACGG CCAAACTCTC ACAAATGGAG
    CAGTACAACA AAGAAATCAA GTTTGTTGAA ATGGATGACT GTGTGCTACA AGGACTTCTC
    TCTTTGCTGG GCTGCTTAAC CAATCTCTTC CTTCTAATAA AGGAATTGAG ACAGAAGCAG
    AGCCAGCAGC AGGACGTCCT GGAGCAGCTC AGGAGGGTCA GATCTGACAA ACTCAAAGAG
    CTCCAGAGAC ACAGGAAAGA GGAAGAGGAG AGAAAGAGGA AGAAAGAAGA GGAGGCCGCC
    AGACAGGCCC AGTTGGAGCA GGAGCGGCTG GAGCAGGAGG AGGCTGAGCG GCAAAGGAAA
    CTTGCACAGG AACGAGAGGC CAAACTCAGG GAAGAGCAGC AGCGGCAGGC TCTGGCCAGA
    CTGCTGGAGG CTCAGGAGCA GGCCAGAGCC AAAGAGGAGA AGAGGAGAGC TGAGGAGAAG
    GAGAGGAATG AGCTAGATGT ACACACTCAA TCCAGTCTGC CTTTCAACAG CACAGAGAAC
    AACATTTTGC AGCCAGTAGT GATCCCTGAA GTCACGTGTA GTTCTCTGAC CACCTACAGA
    GCTCTCTATC CCTTCACTGC ACGCAACTCA GACGAGCTCA CGCTGGAGGC AGACTGTTTC
    ATCCAGGTGG ATGAATCGAC AGTCAGAGAG ACCGGCTGGC TGTATGGCAG TTACAGTGGA
    AACAGAGGCT GGTTTCCAGA GAGCTATGCT GAGAGATGCA GTAAAGACAG TCAGACTCAA
    CCGACCGCAG CTGAAACTGA GTCCACTGCG CCCTCTGCCA ACTACCCTGG AATTCCTCGA
    GTGGACATAG AAGGACCAAC ACCGACACAC ACCCCAGTAT CCACAAACAC ACAGCACTCA
    CAGGCTGTTG CTCTGTGTGA GTGGAGCGCT AAGACTGACA GTCACCTGGG CTTCTGCAAG
    GACGACATTA TTACAGTTCT GGAGAAGCAG GAGAACTGGT GGTACGGAGA ACTCAAGGAG
    AAACGGGGCT GGTTCCCCTG TTCACATGTC TCTGTGGTCA CCACCAATAA TACACAGAGC
    GAGCCTCTGT ATTCTAGCGT GGAGGAGATT GAAGTGTCAG ACTCTGGTTT GCTTGATGAG
    TATGTGGCTC TGTACACGTA TGAGAGTCCG GAGTCAGGAG ACCTGACGTT CAGTGCAGAG
    GATGTGGTTC TGGTTACAGA GAAAGAAGGA GAGTGGTGGA GAGGCTGTAT TGGTGACCAG
    ACCGGCCTTT TCCCCTCCAA CTATGTTAAA CCCAAAGAAC CTGATACTGC CAGTTCTGCA
    GTTCCCAGTA AAAAGCCAGA AATTGCTCAG GTGACTACAG TCAGTCCTGC AACAACACCA
    GAGCAGCTGA GTCTGACACC TGGCCAGCTG ATCGTGGTGC TGCATAAGAA CTCAAACAGC
    TGGTGGCTTG GTGAACTACA GGCACGGGGT AAGAAGCGTA AAAAGGGCTG GTTTCAATCT
    TCTAATGTCC GATTACTGGA GGCCAACAGC GGCAAAATAA CACCTGCACC TCAACCATTG
    TGTCAGGTTA TTGCAATGTA TGACTACAAA GCAGCCAATA AGGACGAGAT GAGCTTCCAG
    AAAGGTCAGC TGATCACCGT ACTGAACAAG GACAACCCTG ACTGGTGGAA AGGAGAGGTC
    GCAGGGGTCA CAGGGCTGTT TCCAACTAAT TATGTGAAGA TGACCACAGA ATGTGATCCA
    AGCCAGCAAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44865
    Clone ID Related Accession (Same CDS sequence) XM_009300808.2
    Accession Version XM_009300808.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4950bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product intersectin 2a isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300808.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATGGTG GCTTTTCTAT TTGGGCTATA ACTCCAGAAG AGAGGGGGAA ACATGACAAA 
    CAGTTTGACT CGCTGGCTCC CACCCTGGGA TATTTGTCGG GAGATCAAGC AAGAAAGTTC
    TTCCTCCAAT CTGGTCTTCC CACATCGGTC CTGGCAGACA TCTGGGCTCT GGCGGACATA
    GGAAAGGATG GGAAGATGGA CAGATTGGAG TTCTCTATTG CAATGAAACT AATTAAACTG
    CAGCTGCAGG GTCAGCCACT TCCGTCCAGC CTGCCGATCA TCATGAAGCA GACGCCCACC
    CCTGTGATGA CGTCATCGTC ACGCTTCGGT ATGGGCTCCA TGCCAAATCT CTCCGCCATG
    TCGGTGATGC CCATCCTAAC ACCCATCCCC ACATGCCCTG TCATGGCCCC TTTAGTTCCT
    GTGCCCACCC TGATATCTGG GCCACTGCCT CAGATGCCCA ACTCTATCAG TGTGCCAGCC
    TTACCTAACG GCAATGCAAC TCTCCTCACA CCAACACCAG CATTCCCAGT CTCCAGTGGT
    TTGACTAAAT CTCACTCTCT CCTGGACCTT GGCTCTAGCA GTTCTAACTC GTCCTCCACC
    ACCTCCCTTG CGAGTAACTC CCCGAAAACG AGTAGTTCTG ATTGGGCAGT GCCTCAGTCA
    TCCCGTCTGA AGTACCGACA ACTGTTTAAC AGCCTGGACA AGCTAATGAG TGGATACCTG
    TCAGGTCCCC AGGTCAGAAA TGCCCTCACA GCTTCAAACC TCACACAAAC ACAGCTTGCC
    ACTATATGGT TTCTGGCTGA TGTGGATCGT GATGGGCAGT TGAGAGCAGA AGAGTTTATT
    TTAGCGATGC ATCTGGTTGA AATGGCCAAG ACAGGGCGGC CTTTACCTCT CACTTTACCT
    CCCGACCTTG TGCCTCCTTC ACTCAGAACA ACTAAGTCAT GTGACCTGCT AAACGGACCT
    GTTCCTCTGA TTAGCACTGA GCTGATTGAG CCAGAACTGC CACAGAAAAA CAAGAGCAAT
    GTTTCATATG AAGACAAACT GAGGGAAAAT TTCCAGAGGG GAAACGCAGA GCTGGAGAAG
    CGTCGCCTGG CTCTTCAGGA GCAGCAGAGG AGAGAGGAGG AGCGCAGGAG AGAAGAGGAG
    AGGCGAGAGG AGGAGAAGAG ACAGCAGAAG GAAAGAGAGG AGCGAGAGAG GAGAGAGAGA
    GAAGCGCGAG AAATGGAGAT CAGGAGACAG AGAGAGGAGG AGCGCAGGAT GGAGAGACAG
    AGAGAGCTGG AGCGACAGAG AGAGGAGGAA CGACTGAAAG AATTAGAGCG GCGAGAGGCA
    AGTAGAGCAA GCAAGGACAT ACAGAGGGAA GCTGAGCGAC AAAGACGGAG GGAATGGGAG
    AAGAGCAAAC GTGTGGAGCT TCAGACTCAG AAAGAAAAGG AGCAGAATGA CATACAGAGA
    CTAAAGGACA GGAAGAGGAG TCTTGAGATT GAGCTGGAGG CCGTGGGTAA CAAGCATAAG
    CAGATCTCCA GTCAGCTGCG TGATGCTAAG AGTAAGAGAC AGGGGCAGTG GGCCGAGTTG
    GAGCTGATCA ACCAGAGGAG AGACGTCTGC ATTACTGATA TCAACTCACT GCAGTTACAG
    TTTGATGAGA TGCAGCGGCA GTTGAATCAG CTGGGCCCTG AAAAGCAGCG ACTGGCTGAC
    AGACTTGTAC ATTTTACCCA AAACATCTCC AGTCCTTTGA TAAACGATGT GAAACAGAGT
    GTGTGCGAGA AAGATCGGAG CTGTCGGAAG CTTAAGGAGC AGCTGGATGT TTTGGAGAGA
    GAAACCACGG CCAAACTCTC ACAAATGGAG CAGTACAACA AAGAAATCAA GGAATTGAGA
    CAGAAGCAGA GCCAGCAGCA GGACGTCCTG GAGCAGCTCA GGAGGGTCAG ATCTGACAAA
    CTCAAAGAGC TCCAGAGACA CAGGAAAGAG GAAGAGGAGA GAAAGAGGAA GAAAGAAGAG
    GAGGCCGCCA GACAGGCCCA GTTGGAGCAG GAGCGGCTGG AGCAGGAGGA GGCTGAGCGG
    CAAAGGAAAC TTGCACAGGA ACGAGAGGCC AAACTCAGGG AAGAGCAGCA GCGGCAGGCT
    CTGGCCAGAC TGCTGGAGGC TCAGGAGCAG GCCAGAGCCA AAGAGGAGAA GAGGAGAGCT
    GAGGAGAAGG AGAGGAATGA GCTAGATGTA CACACTCAAT CCAGTCTGCC TTTCAACAGC
    ACAGAGAACA ACATTTTGCA GCCAGTAGTG ATCCCTGAAG TCACGTGTAG TTCTCTGACC
    ACCTACAGAG CTCTCTATCC CTTCACTGCA CGCAACTCAG ACGAGCTCAC GCTGGAGGCA
    GACTGTTTCA TCCAGGTGGA TGAATCGACA GTCAGAGAGA CCGGCTGGCT GTATGGCAGT
    TACAGTGGAA ACAGAGGCTG GTTTCCAGAG AGCTATGCTG AGAGATGCAG TAAAGACAGT
    CAGACTCAAC CGACCGCAGC TGAAACTGAG TCCACTGCGC CCTCTGCCAA CTACCCTGGA
    ATTCCTCGAG TGGACATAGA AGGACCAACA CCGACACACA CCCCAGTATC CACAAACACA
    CAGCACTCAC AGGCTGTTGC TCTGTGTGAG TGGAGCGCTA AGACTGACAG TCACCTGGGC
    TTCTGCAAGG ACGACATTAT TACAGTTCTG GAGAAGCAGG AGAACTGGTG GTACGGAGAA
    CTCAAGGAGA AACGGGGCTG GTTCCCCTGT TCACATGTCT CTGTGGTCAC CACCAATAAT
    ACACAGAGCG AGCCTCTGTA TTCTAGCGTG GAGGAGATTG AAGTGTCAGA CTCTGGTTTG
    CTTGATGAGT ATGTGGCTCT GTACACGTAT GAGAGTCCGG AGTCAGGAGA CCTGACGTTC
    AGTGCAGAGG ATGTGGTTCT GGTTACAGAG AAAGAAGGAG AGTGGTGGAG AGGCTGTATT
    GGTGACCAGA CCGGCCTTTT CCCCTCCAAC TATGTTAAAC CCAAAGAACC TGATACTGCC
    AGTTCTGCAG TTCCCAGTAA AAAGCCAGAA ATTGCTCAGG TGACTACAGT CAGTCCTGCA
    ACAACACCAG AGCAGCTGAG TCTGACACCT GGCCAGCTGA TCGTGGTGCT GCATAAGAAC
    TCAAACAGCT GGTGGCTTGG TGAACTACAG GCACGGGGTA AGAAGCGTAA AAAGGGCTGG
    TTTCAATCTT CTAATGTCCG ATTACTGGAG GCCAACAGCG GCAAAATAAC ACCTGCACCT
    CAACCATTGT GTCAGGTTAT TGCAATGTAT GACTACAAAG CAGCCAATAA GGACGAGATG
    AGCTTCCAGA AAGGTCAGCT GATCACCGTA CTGAACAAGG ACAACCCTGA CTGGTGGAAA
    GGAGAGGTCG CAGGGGTCAC AGGGCTGTTT CCAACTAATT ATGTGAAGAT GACCACAGAA
    TGTGATCCAA GCCAGCAATG GTGTGCTGAC CTGCTTAGTT TAGACTCTAT GTGCACTGAG
    GAGAGGAAGA GGCAAGGCTA CATCCATGAG CTCATTCAGA CAGAAGAGTC TTACCTGGAG
    GACCTGGAGC TGGCAGTGGA GGTGTTTTAT AAGCCAATGG CAGAGTCGGG ACGGCTGACC
    GAGGCTGAGA TCAGCATGAT CTTTGTGAAC TGGCGTGAAT TGATCATGTG CAGCACCAAA
    CTCCTAAAAG CTCTGCGTGT GCGTAAGAAG ACAGCAGGTG AGCGAATGCC CGTGCAGGTG
    GTTGGTGATA TCTTGTCCTC TGAGCTCTCT CACATGCAGG CTTACATTCG CTTCTGCAGC
    TGCCAGCTCA ACGCAGCTGC TCTGTTGCAG CAGAAAACAG ACAAATCACT CGACTTCAAA
    CTCTTCCTCA AGAAAATTGC CAGTAATTAC CGCTGTAAAG GAATGCCACT GTCCAGCTTT
    CTCCTGAAGC CCATGCAGAG GATCACACGA TACCCACTGC TGATCAAAAA CATTCTGGAA
    AACACTCCTC CGGCTCACGC TGACCACTCC AATCTGCAGG CGGCGCTGGA GCAGGCGGAG
    GAGCTTTGCT CACAGGTGAA CGAGGCTGTG AGGGAGAAAG AGAACTCCGA CCGACTGGAG
    TGGATCCAGA ACCACGTGTT GTGTGACGGA GTCATTGAGC ACCTTGTTTT TAACTCTCTG
    ACGAACTGCC TGGGCCCTCG AAAACTGCTG CACAGCGGCA AACTGCATAA GACCAAGAGC
    AGCAAAGAGC TCTGGGCCCT GCTGTTCAAT GACTTCCTGC TCCTCACATA CACCTCCAAA
    CAGTTCTCAT CCAGCCCAGA CAAACTCTTC AATCCCAACT CCAACACACA GTACAAGATG
    TACAAGACGC CAGTGTTTCT GAATGAGGTC TTGGTGAAAA TGCCCTCTGA CCCGTCCAGC
    GATGACCCAG TTTTCCACAT TTCACACATT GATCGTGTTT ACACACTCAA GACTGACACT
    ATCAATGAGA GGACGACGTG GGTGCAGAAG ATCAAAGCAG CATCTGATCA CTTTATAGAG
    ACGGAGAAGC TGAAGAGAGA GAAGGCGTAT CAAGCTCGCT CTCAGAAGAA CAGTGGCATC
    GGTCGACTGC TGGTAACAGT GCTGGAAGCC ACAGAGCTGA AGCCCTGCAA ACCCAACGGG
    AAGAGTAACC CATACTGTGA ACTAACCATG GGCGCCCAGT GCTACACGTC CCGGCATCAG
    CCCGACACGC TCAACCCAAA ATGGAGCTTC AACTGCCAGT TCTTCATCAA AGACTTGTAC
    CAGGATGTCC TGTGCCTCAC TGTCTTTGAG AGGGACCAGT TCTCTCCTGA TGATTTTCTA
    GGTCGAACTG AAGTTCCTGT AGCGACAATA AAAAAAGATC AAGATGGTAA AGGGCCACTG
    GTTCGACGTC TGCTGCTTCA TGAAGTCCCG ACTGGAGAGG TTAAAGTGAG GCTTGATCTG
    CAGCTCTATG AACAAACACC TCACGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299083.1
    CDS205..5154
    Translation

    Target ORF information:

    RefSeq Version XM_009300808.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio intersectin 2a (itsn2a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300808.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAATGGTG GCTTTTCTAT TTGGGCTATA ACTCCAGAAG AGAGGGGGAA ACATGACAAA 
    CAGTTTGACT CGCTGGCTCC CACCCTGGGA TATTTGTCGG GAGATCAAGC AAGAAAGTTC
    TTCCTCCAAT CTGGTCTTCC CACATCGGTC CTGGCAGACA TCTGGGCTCT GGCGGACATA
    GGAAAGGATG GGAAGATGGA CAGATTGGAG TTCTCTATTG CAATGAAACT AATTAAACTG
    CAGCTGCAGG GTCAGCCACT TCCGTCCAGC CTGCCGATCA TCATGAAGCA GACGCCCACC
    CCTGTGATGA CGTCATCGTC ACGCTTCGGT ATGGGCTCCA TGCCAAATCT CTCCGCCATG
    TCGGTGATGC CCATCCTAAC ACCCATCCCC ACATGCCCTG TCATGGCCCC TTTAGTTCCT
    GTGCCCACCC TGATATCTGG GCCACTGCCT CAGATGCCCA ACTCTATCAG TGTGCCAGCC
    TTACCTAACG GCAATGCAAC TCTCCTCACA CCAACACCAG CATTCCCAGT CTCCAGTGGT
    TTGACTAAAT CTCACTCTCT CCTGGACCTT GGCTCTAGCA GTTCTAACTC GTCCTCCACC
    ACCTCCCTTG CGAGTAACTC CCCGAAAACG AGTAGTTCTG ATTGGGCAGT GCCTCAGTCA
    TCCCGTCTGA AGTACCGACA ACTGTTTAAC AGCCTGGACA AGCTAATGAG TGGATACCTG
    TCAGGTCCCC AGGTCAGAAA TGCCCTCACA GCTTCAAACC TCACACAAAC ACAGCTTGCC
    ACTATATGGT TTCTGGCTGA TGTGGATCGT GATGGGCAGT TGAGAGCAGA AGAGTTTATT
    TTAGCGATGC ATCTGGTTGA AATGGCCAAG ACAGGGCGGC CTTTACCTCT CACTTTACCT
    CCCGACCTTG TGCCTCCTTC ACTCAGAACA ACTAAGTCAT GTGACCTGCT AAACGGACCT
    GTTCCTCTGA TTAGCACTGA GCTGATTGAG CCAGAACTGC CACAGAAAAA CAAGAGCAAT
    GTTTCATATG AAGACAAACT GAGGGAAAAT TTCCAGAGGG GAAACGCAGA GCTGGAGAAG
    CGTCGCCTGG CTCTTCAGGA GCAGCAGAGG AGAGAGGAGG AGCGCAGGAG AGAAGAGGAG
    AGGCGAGAGG AGGAGAAGAG ACAGCAGAAG GAAAGAGAGG AGCGAGAGAG GAGAGAGAGA
    GAAGCGCGAG AAATGGAGAT CAGGAGACAG AGAGAGGAGG AGCGCAGGAT GGAGAGACAG
    AGAGAGCTGG AGCGACAGAG AGAGGAGGAA CGACTGAAAG AATTAGAGCG GCGAGAGGCA
    AGTAGAGCAA GCAAGGACAT ACAGAGGGAA GCTGAGCGAC AAAGACGGAG GGAATGGGAG
    AAGAGCAAAC GTGTGGAGCT TCAGACTCAG AAAGAAAAGG AGCAGAATGA CATACAGAGA
    CTAAAGGACA GGAAGAGGAG TCTTGAGATT GAGCTGGAGG CCGTGGGTAA CAAGCATAAG
    CAGATCTCCA GTCAGCTGCG TGATGCTAAG AGTAAGAGAC AGGGGCAGTG GGCCGAGTTG
    GAGCTGATCA ACCAGAGGAG AGACGTCTGC ATTACTGATA TCAACTCACT GCAGTTACAG
    TTTGATGAGA TGCAGCGGCA GTTGAATCAG CTGGGCCCTG AAAAGCAGCG ACTGGCTGAC
    AGACTTGTAC ATTTTACCCA AAACATCTCC AGTCCTTTGA TAAACGATGT GAAACAGAGT
    GTGTGCGAGA AAGATCGGAG CTGTCGGAAG CTTAAGGAGC AGCTGGATGT TTTGGAGAGA
    GAAACCACGG CCAAACTCTC ACAAATGGAG CAGTACAACA AAGAAATCAA GGAATTGAGA
    CAGAAGCAGA GCCAGCAGCA GGACGTCCTG GAGCAGCTCA GGAGGGTCAG ATCTGACAAA
    CTCAAAGAGC TCCAGAGACA CAGGAAAGAG GAAGAGGAGA GAAAGAGGAA GAAAGAAGAG
    GAGGCCGCCA GACAGGCCCA GTTGGAGCAG GAGCGGCTGG AGCAGGAGGA GGCTGAGCGG
    CAAAGGAAAC TTGCACAGGA ACGAGAGGCC AAACTCAGGG AAGAGCAGCA GCGGCAGGCT
    CTGGCCAGAC TGCTGGAGGC TCAGGAGCAG GCCAGAGCCA AAGAGGAGAA GAGGAGAGCT
    GAGGAGAAGG AGAGGAATGA GCTAGATGTA CACACTCAAT CCAGTCTGCC TTTCAACAGC
    ACAGAGAACA ACATTTTGCA GCCAGTAGTG ATCCCTGAAG TCACGTGTAG TTCTCTGACC
    ACCTACAGAG CTCTCTATCC CTTCACTGCA CGCAACTCAG ACGAGCTCAC GCTGGAGGCA
    GACTGTTTCA TCCAGGTGGA TGAATCGACA GTCAGAGAGA CCGGCTGGCT GTATGGCAGT
    TACAGTGGAA ACAGAGGCTG GTTTCCAGAG AGCTATGCTG AGAGATGCAG TAAAGACAGT
    CAGACTCAAC CGACCGCAGC TGAAACTGAG TCCACTGCGC CCTCTGCCAA CTACCCTGGA
    ATTCCTCGAG TGGACATAGA AGGACCAACA CCGACACACA CCCCAGTATC CACAAACACA
    CAGCACTCAC AGGCTGTTGC TCTGTGTGAG TGGAGCGCTA AGACTGACAG TCACCTGGGC
    TTCTGCAAGG ACGACATTAT TACAGTTCTG GAGAAGCAGG AGAACTGGTG GTACGGAGAA
    CTCAAGGAGA AACGGGGCTG GTTCCCCTGT TCACATGTCT CTGTGGTCAC CACCAATAAT
    ACACAGAGCG AGCCTCTGTA TTCTAGCGTG GAGGAGATTG AAGTGTCAGA CTCTGGTTTG
    CTTGATGAGT ATGTGGCTCT GTACACGTAT GAGAGTCCGG AGTCAGGAGA CCTGACGTTC
    AGTGCAGAGG ATGTGGTTCT GGTTACAGAG AAAGAAGGAG AGTGGTGGAG AGGCTGTATT
    GGTGACCAGA CCGGCCTTTT CCCCTCCAAC TATGTTAAAC CCAAAGAACC TGATACTGCC
    AGTTCTGCAG TTCCCAGTAA AAAGCCAGAA ATTGCTCAGG TGACTACAGT CAGTCCTGCA
    ACAACACCAG AGCAGCTGAG TCTGACACCT GGCCAGCTGA TCGTGGTGCT GCATAAGAAC
    TCAAACAGCT GGTGGCTTGG TGAACTACAG GCACGGGGTA AGAAGCGTAA AAAGGGCTGG
    TTTCAATCTT CTAATGTCCG ATTACTGGAG GCCAACAGCG GCAAAATAAC ACCTGCACCT
    CAACCATTGT GTCAGGTTAT TGCAATGTAT GACTACAAAG CAGCCAATAA GGACGAGATG
    AGCTTCCAGA AAGGTCAGCT GATCACCGTA CTGAACAAGG ACAACCCTGA CTGGTGGAAA
    GGAGAGGTCG CAGGGGTCAC AGGGCTGTTT CCAACTAATT ATGTGAAGAT GACCACAGAA
    TGTGATCCAA GCCAGCAATG GTGTGCTGAC CTGCTTAGTT TAGACTCTAT GTGCACTGAG
    GAGAGGAAGA GGCAAGGCTA CATCCATGAG CTCATTCAGA CAGAAGAGTC TTACCTGGAG
    GACCTGGAGC TGGCAGTGGA GGTGTTTTAT AAGCCAATGG CAGAGTCGGG ACGGCTGACC
    GAGGCTGAGA TCAGCATGAT CTTTGTGAAC TGGCGTGAAT TGATCATGTG CAGCACCAAA
    CTCCTAAAAG CTCTGCGTGT GCGTAAGAAG ACAGCAGGTG AGCGAATGCC CGTGCAGGTG
    GTTGGTGATA TCTTGTCCTC TGAGCTCTCT CACATGCAGG CTTACATTCG CTTCTGCAGC
    TGCCAGCTCA ACGCAGCTGC TCTGTTGCAG CAGAAAACAG ACAAATCACT CGACTTCAAA
    CTCTTCCTCA AGAAAATTGC CAGTAATTAC CGCTGTAAAG GAATGCCACT GTCCAGCTTT
    CTCCTGAAGC CCATGCAGAG GATCACACGA TACCCACTGC TGATCAAAAA CATTCTGGAA
    AACACTCCTC CGGCTCACGC TGACCACTCC AATCTGCAGG CGGCGCTGGA GCAGGCGGAG
    GAGCTTTGCT CACAGGTGAA CGAGGCTGTG AGGGAGAAAG AGAACTCCGA CCGACTGGAG
    TGGATCCAGA ACCACGTGTT GTGTGACGGA GTCATTGAGC ACCTTGTTTT TAACTCTCTG
    ACGAACTGCC TGGGCCCTCG AAAACTGCTG CACAGCGGCA AACTGCATAA GACCAAGAGC
    AGCAAAGAGC TCTGGGCCCT GCTGTTCAAT GACTTCCTGC TCCTCACATA CACCTCCAAA
    CAGTTCTCAT CCAGCCCAGA CAAACTCTTC AATCCCAACT CCAACACACA GTACAAGATG
    TACAAGACGC CAGTGTTTCT GAATGAGGTC TTGGTGAAAA TGCCCTCTGA CCCGTCCAGC
    GATGACCCAG TTTTCCACAT TTCACACATT GATCGTGTTT ACACACTCAA GACTGACACT
    ATCAATGAGA GGACGACGTG GGTGCAGAAG ATCAAAGCAG CATCTGATCA CTTTATAGAG
    ACGGAGAAGC TGAAGAGAGA GAAGGCGTAT CAAGCTCGCT CTCAGAAGAA CAGTGGCATC
    GGTCGACTGC TGGTAACAGT GCTGGAAGCC ACAGAGCTGA AGCCCTGCAA ACCCAACGGG
    AAGAGTAACC CATACTGTGA ACTAACCATG GGCGCCCAGT GCTACACGTC CCGGCATCAG
    CCCGACACGC TCAACCCAAA ATGGAGCTTC AACTGCCAGT TCTTCATCAA AGACTTGTAC
    CAGGATGTCC TGTGCCTCAC TGTCTTTGAG AGGGACCAGT TCTCTCCTGA TGATTTTCTA
    GGTCGAACTG AAGTTCCTGT AGCGACAATA AAAAAAGATC AAGATGGTAA AGGGCCACTG
    GTTCGACGTC TGCTGCTTCA TGAAGTCCCG ACTGGAGAGG TTAAAGTGAG GCTTGATCTG
    CAGCTCTATG AACAAACACC TCACGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44866
    Clone ID Related Accession (Same CDS sequence) XM_009300809.3 , XM_009300809.2
    Accession Version XM_009300809.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4920bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product intersectin 2a isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300809.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATGGTG GCTTTTCTAT TTGGGCTATA ACTCCAGAAG AGAGGGGGAA ACATGACAAA 
    CAGTTTGACT CGCTGGCTCC CACCCTGGGA TATTTGTCGG GAGATCAAGC AAGAAAGTTC
    TTCCTCCAAT CTGGTCTTCC CACATCGGTC CTGGCAGACA TCTGGGCTCT GGCGGACATA
    GGAAAGGATG GGAAGATGGA CAGATTGGAG TTCTCTATTG CAATGAAACT AATTAAACTG
    CAGCTGCAGG GTCAGCCACT TCCGTCCAGC CTGCCGATCA TCATGAAGCA GACGCCCACC
    CCTGTGATGA CGTCATCGTC ACGCTTCGGT ATGGGCTCCA TGCCAAATCT CTCCGCCATG
    TCGGTGATGC CCATCCTAAC ACCCATCCCC ACATGCCCTG TCATGGCCCC TTTAGTTCCT
    GTGCCCACCC TGATATCTGG GCCACTGCCT CAGATGCCCA ACTCTATCAG TGTGCCAGCC
    TTACCTAACG GCAATGCAAC TCTCCTCACA CCAACACCAG CATTCCCAGT CTCCAGTGGT
    TTGACTAAAT CTCACTCTCT CCTGGACCTT GGCTCTAGCA GTTCTAACTC GTCCTCCACC
    ACCTCCCTTG CGAGTAACTC CCCGAAAACG AGTAGTTCTG ATTGGGCAGT GCCTCAGTCA
    TCCCGTCTGA AGTACCGACA ACTGTTTAAC AGCCTGGACA AGCTAATGAG TGGATACCTG
    TCAGGTCCCC AGGTCAGAAA TGCCCTCACA GCTTCAAACC TCACACAAAC ACAGCTTGCC
    ACTATATGGT TTCTGGCTGA TGTGGATCGT GATGGGCAGT TGAGAGCAGA AGAGTTTATT
    TTAGCGATGC ATCTGGTTGA AATGGCCAAG ACAGGGCGGC CTTTACCTCT CACTTTACCT
    CCCGACCTTG TGCCTCCTTC ACTCAGAACA ACTAAGTCAT GTGACCTGCT AAACGGACCT
    GTTCCTCTGA TTAGCACTGA GCTGATTGAG CCAGAACTGC CACAGAAAAA CAAGAGCAAT
    GTTTCATATG AAGACAAACT GAGGGAAAAT TTCCAGAGGG GAAACGCAGA GCTGGAGAAG
    CGTCGCCTGG CTCTTCAGGA GCAGCAGAGG AGAGAGGAGG AGCGCAGGAG AGAAGAGGAG
    AGGCGAGAGG AGGAGAAGAG ACAGCAGAAG GAAAGAGAGG AGCGAGAGAG GAGAGAGAGA
    GAAGCGCGAG AAATGGAGAT CAGGAGACAG AGAGAGGAGG AGCGCAGGAT GGAGAGACAG
    AGAGAGCTGG AGCGACAGAG AGAGGAGGAA CGACTGAAAG AATTAGAGCG GCGAGAGGAA
    GCTGAGCGAC AAAGACGGAG GGAATGGGAG AAGAGCAAAC GTGTGGAGCT TCAGACTCAG
    AAAGAAAAGG AGCAGAATGA CATACAGAGA CTAAAGGACA GGAAGAGGAG TCTTGAGATT
    GAGCTGGAGG CCGTGGGTAA CAAGCATAAG CAGATCTCCA GTCAGCTGCG TGATGCTAAG
    AGTAAGAGAC AGGGGCAGTG GGCCGAGTTG GAGCTGATCA ACCAGAGGAG AGACGTCTGC
    ATTACTGATA TCAACTCACT GCAGTTACAG TTTGATGAGA TGCAGCGGCA GTTGAATCAG
    CTGGGCCCTG AAAAGCAGCG ACTGGCTGAC AGACTTGTAC ATTTTACCCA AAACATCTCC
    AGTCCTTTGA TAAACGATGT GAAACAGAGT GTGTGCGAGA AAGATCGGAG CTGTCGGAAG
    CTTAAGGAGC AGCTGGATGT TTTGGAGAGA GAAACCACGG CCAAACTCTC ACAAATGGAG
    CAGTACAACA AAGAAATCAA GGAATTGAGA CAGAAGCAGA GCCAGCAGCA GGACGTCCTG
    GAGCAGCTCA GGAGGGTCAG ATCTGACAAA CTCAAAGAGC TCCAGAGACA CAGGAAAGAG
    GAAGAGGAGA GAAAGAGGAA GAAAGAAGAG GAGGCCGCCA GACAGGCCCA GTTGGAGCAG
    GAGCGGCTGG AGCAGGAGGA GGCTGAGCGG CAAAGGAAAC TTGCACAGGA ACGAGAGGCC
    AAACTCAGGG AAGAGCAGCA GCGGCAGGCT CTGGCCAGAC TGCTGGAGGC TCAGGAGCAG
    GCCAGAGCCA AAGAGGAGAA GAGGAGAGCT GAGGAGAAGG AGAGGAATGA GCTAGATGTA
    CACACTCAAT CCAGTCTGCC TTTCAACAGC ACAGAGAACA ACATTTTGCA GCCAGTAGTG
    ATCCCTGAAG TCACGTGTAG TTCTCTGACC ACCTACAGAG CTCTCTATCC CTTCACTGCA
    CGCAACTCAG ACGAGCTCAC GCTGGAGGCA GACTGTTTCA TCCAGGTGGA TGAATCGACA
    GTCAGAGAGA CCGGCTGGCT GTATGGCAGT TACAGTGGAA ACAGAGGCTG GTTTCCAGAG
    AGCTATGCTG AGAGATGCAG TAAAGACAGT CAGACTCAAC CGACCGCAGC TGAAACTGAG
    TCCACTGCGC CCTCTGCCAA CTACCCTGGA ATTCCTCGAG TGGACATAGA AGGACCAACA
    CCGACACACA CCCCAGTATC CACAAACACA CAGCACTCAC AGGCTGTTGC TCTGTGTGAG
    TGGAGCGCTA AGACTGACAG TCACCTGGGC TTCTGCAAGG ACGACATTAT TACAGTTCTG
    GAGAAGCAGG AGAACTGGTG GTACGGAGAA CTCAAGGAGA AACGGGGCTG GTTCCCCTGT
    TCACATGTCT CTGTGGTCAC CACCAATAAT ACACAGAGCG AGCCTCTGTA TTCTAGCGTG
    GAGGAGATTG AAGTGTCAGA CTCTGGTTTG CTTGATGAGT ATGTGGCTCT GTACACGTAT
    GAGAGTCCGG AGTCAGGAGA CCTGACGTTC AGTGCAGAGG ATGTGGTTCT GGTTACAGAG
    AAAGAAGGAG AGTGGTGGAG AGGCTGTATT GGTGACCAGA CCGGCCTTTT CCCCTCCAAC
    TATGTTAAAC CCAAAGAACC TGATACTGCC AGTTCTGCAG TTCCCAGTAA AAAGCCAGAA
    ATTGCTCAGG TGACTACAGT CAGTCCTGCA ACAACACCAG AGCAGCTGAG TCTGACACCT
    GGCCAGCTGA TCGTGGTGCT GCATAAGAAC TCAAACAGCT GGTGGCTTGG TGAACTACAG
    GCACGGGGTA AGAAGCGTAA AAAGGGCTGG TTTCAATCTT CTAATGTCCG ATTACTGGAG
    GCCAACAGCG GCAAAATAAC ACCTGCACCT CAACCATTGT GTCAGGTTAT TGCAATGTAT
    GACTACAAAG CAGCCAATAA GGACGAGATG AGCTTCCAGA AAGGTCAGCT GATCACCGTA
    CTGAACAAGG ACAACCCTGA CTGGTGGAAA GGAGAGGTCG CAGGGGTCAC AGGGCTGTTT
    CCAACTAATT ATGTGAAGAT GACCACAGAA TGTGATCCAA GCCAGCAATG GTGTGCTGAC
    CTGCTTAGTT TAGACTCTAT GTGCACTGAG GAGAGGAAGA GGCAAGGCTA CATCCATGAG
    CTCATTCAGA CAGAAGAGTC TTACCTGGAG GACCTGGAGC TGGCAGTGGA GGTGTTTTAT
    AAGCCAATGG CAGAGTCGGG ACGGCTGACC GAGGCTGAGA TCAGCATGAT CTTTGTGAAC
    TGGCGTGAAT TGATCATGTG CAGCACCAAA CTCCTAAAAG CTCTGCGTGT GCGTAAGAAG
    ACAGCAGGTG AGCGAATGCC CGTGCAGGTG GTTGGTGATA TCTTGTCCTC TGAGCTCTCT
    CACATGCAGG CTTACATTCG CTTCTGCAGC TGCCAGCTCA ACGCAGCTGC TCTGTTGCAG
    CAGAAAACAG ACAAATCACT CGACTTCAAA CTCTTCCTCA AGAAAATTGC CAGTAATTAC
    CGCTGTAAAG GAATGCCACT GTCCAGCTTT CTCCTGAAGC CCATGCAGAG GATCACACGA
    TACCCACTGC TGATCAAAAA CATTCTGGAA AACACTCCTC CGGCTCACGC TGACCACTCC
    AATCTGCAGG CGGCGCTGGA GCAGGCGGAG GAGCTTTGCT CACAGGTGAA CGAGGCTGTG
    AGGGAGAAAG AGAACTCCGA CCGACTGGAG TGGATCCAGA ACCACGTGTT GTGTGACGGA
    GTCATTGAGC ACCTTGTTTT TAACTCTCTG ACGAACTGCC TGGGCCCTCG AAAACTGCTG
    CACAGCGGCA AACTGCATAA GACCAAGAGC AGCAAAGAGC TCTGGGCCCT GCTGTTCAAT
    GACTTCCTGC TCCTCACATA CACCTCCAAA CAGTTCTCAT CCAGCCCAGA CAAACTCTTC
    AATCCCAACT CCAACACACA GTACAAGATG TACAAGACGC CAGTGTTTCT GAATGAGGTC
    TTGGTGAAAA TGCCCTCTGA CCCGTCCAGC GATGACCCAG TTTTCCACAT TTCACACATT
    GATCGTGTTT ACACACTCAA GACTGACACT ATCAATGAGA GGACGACGTG GGTGCAGAAG
    ATCAAAGCAG CATCTGATCA CTTTATAGAG ACGGAGAAGC TGAAGAGAGA GAAGGCGTAT
    CAAGCTCGCT CTCAGAAGAA CAGTGGCATC GGTCGACTGC TGGTAACAGT GCTGGAAGCC
    ACAGAGCTGA AGCCCTGCAA ACCCAACGGG AAGAGTAACC CATACTGTGA ACTAACCATG
    GGCGCCCAGT GCTACACGTC CCGGCATCAG CCCGACACGC TCAACCCAAA ATGGAGCTTC
    AACTGCCAGT TCTTCATCAA AGACTTGTAC CAGGATGTCC TGTGCCTCAC TGTCTTTGAG
    AGGGACCAGT TCTCTCCTGA TGATTTTCTA GGTCGAACTG AAGTTCCTGT AGCGACAATA
    AAAAAAGATC AAGATGGTAA AGGGCCACTG GTTCGACGTC TGCTGCTTCA TGAAGTCCCG
    ACTGGAGAGG TTAAAGTGAG GCTTGATCTG CAGCTCTATG AACAAACACC TCACGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299084.1
    CDS329..5248
    Translation

    Target ORF information:

    RefSeq Version XM_009300809.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio intersectin 2a (itsn2a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300809.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATGGTG GCTTTTCTAT TTGGGCTATA ACTCCAGAAG AGAGGGGGAA ACATGACAAA 
    CAGTTTGACT CGCTGGCTCC CACCCTGGGA TATTTGTCGG GAGATCAAGC AAGAAAGTTC
    TTCCTCCAAT CTGGTCTTCC CACATCGGTC CTGGCAGACA TCTGGGCTCT GGCGGACATA
    GGAAAGGATG GGAAGATGGA CAGATTGGAG TTCTCTATTG CAATGAAACT AATTAAACTG
    CAGCTGCAGG GTCAGCCACT TCCGTCCAGC CTGCCGATCA TCATGAAGCA GACGCCCACC
    CCTGTGATGA CGTCATCGTC ACGCTTCGGT ATGGGCTCCA TGCCAAATCT CTCCGCCATG
    TCGGTGATGC CCATCCTAAC ACCCATCCCC ACATGCCCTG TCATGGCCCC TTTAGTTCCT
    GTGCCCACCC TGATATCTGG GCCACTGCCT CAGATGCCCA ACTCTATCAG TGTGCCAGCC
    TTACCTAACG GCAATGCAAC TCTCCTCACA CCAACACCAG CATTCCCAGT CTCCAGTGGT
    TTGACTAAAT CTCACTCTCT CCTGGACCTT GGCTCTAGCA GTTCTAACTC GTCCTCCACC
    ACCTCCCTTG CGAGTAACTC CCCGAAAACG AGTAGTTCTG ATTGGGCAGT GCCTCAGTCA
    TCCCGTCTGA AGTACCGACA ACTGTTTAAC AGCCTGGACA AGCTAATGAG TGGATACCTG
    TCAGGTCCCC AGGTCAGAAA TGCCCTCACA GCTTCAAACC TCACACAAAC ACAGCTTGCC
    ACTATATGGT TTCTGGCTGA TGTGGATCGT GATGGGCAGT TGAGAGCAGA AGAGTTTATT
    TTAGCGATGC ATCTGGTTGA AATGGCCAAG ACAGGGCGGC CTTTACCTCT CACTTTACCT
    CCCGACCTTG TGCCTCCTTC ACTCAGAACA ACTAAGTCAT GTGACCTGCT AAACGGACCT
    GTTCCTCTGA TTAGCACTGA GCTGATTGAG CCAGAACTGC CACAGAAAAA CAAGAGCAAT
    GTTTCATATG AAGACAAACT GAGGGAAAAT TTCCAGAGGG GAAACGCAGA GCTGGAGAAG
    CGTCGCCTGG CTCTTCAGGA GCAGCAGAGG AGAGAGGAGG AGCGCAGGAG AGAAGAGGAG
    AGGCGAGAGG AGGAGAAGAG ACAGCAGAAG GAAAGAGAGG AGCGAGAGAG GAGAGAGAGA
    GAAGCGCGAG AAATGGAGAT CAGGAGACAG AGAGAGGAGG AGCGCAGGAT GGAGAGACAG
    AGAGAGCTGG AGCGACAGAG AGAGGAGGAA CGACTGAAAG AATTAGAGCG GCGAGAGGAA
    GCTGAGCGAC AAAGACGGAG GGAATGGGAG AAGAGCAAAC GTGTGGAGCT TCAGACTCAG
    AAAGAAAAGG AGCAGAATGA CATACAGAGA CTAAAGGACA GGAAGAGGAG TCTTGAGATT
    GAGCTGGAGG CCGTGGGTAA CAAGCATAAG CAGATCTCCA GTCAGCTGCG TGATGCTAAG
    AGTAAGAGAC AGGGGCAGTG GGCCGAGTTG GAGCTGATCA ACCAGAGGAG AGACGTCTGC
    ATTACTGATA TCAACTCACT GCAGTTACAG TTTGATGAGA TGCAGCGGCA GTTGAATCAG
    CTGGGCCCTG AAAAGCAGCG ACTGGCTGAC AGACTTGTAC ATTTTACCCA AAACATCTCC
    AGTCCTTTGA TAAACGATGT GAAACAGAGT GTGTGCGAGA AAGATCGGAG CTGTCGGAAG
    CTTAAGGAGC AGCTGGATGT TTTGGAGAGA GAAACCACGG CCAAACTCTC ACAAATGGAG
    CAGTACAACA AAGAAATCAA GGAATTGAGA CAGAAGCAGA GCCAGCAGCA GGACGTCCTG
    GAGCAGCTCA GGAGGGTCAG ATCTGACAAA CTCAAAGAGC TCCAGAGACA CAGGAAAGAG
    GAAGAGGAGA GAAAGAGGAA GAAAGAAGAG GAGGCCGCCA GACAGGCCCA GTTGGAGCAG
    GAGCGGCTGG AGCAGGAGGA GGCTGAGCGG CAAAGGAAAC TTGCACAGGA ACGAGAGGCC
    AAACTCAGGG AAGAGCAGCA GCGGCAGGCT CTGGCCAGAC TGCTGGAGGC TCAGGAGCAG
    GCCAGAGCCA AAGAGGAGAA GAGGAGAGCT GAGGAGAAGG AGAGGAATGA GCTAGATGTA
    CACACTCAAT CCAGTCTGCC TTTCAACAGC ACAGAGAACA ACATTTTGCA GCCAGTAGTG
    ATCCCTGAAG TCACGTGTAG TTCTCTGACC ACCTACAGAG CTCTCTATCC CTTCACTGCA
    CGCAACTCAG ACGAGCTCAC GCTGGAGGCA GACTGTTTCA TCCAGGTGGA TGAATCGACA
    GTCAGAGAGA CCGGCTGGCT GTATGGCAGT TACAGTGGAA ACAGAGGCTG GTTTCCAGAG
    AGCTATGCTG AGAGATGCAG TAAAGACAGT CAGACTCAAC CGACCGCAGC TGAAACTGAG
    TCCACTGCGC CCTCTGCCAA CTACCCTGGA ATTCCTCGAG TGGACATAGA AGGACCAACA
    CCGACACACA CCCCAGTATC CACAAACACA CAGCACTCAC AGGCTGTTGC TCTGTGTGAG
    TGGAGCGCTA AGACTGACAG TCACCTGGGC TTCTGCAAGG ACGACATTAT TACAGTTCTG
    GAGAAGCAGG AGAACTGGTG GTACGGAGAA CTCAAGGAGA AACGGGGCTG GTTCCCCTGT
    TCACATGTCT CTGTGGTCAC CACCAATAAT ACACAGAGCG AGCCTCTGTA TTCTAGCGTG
    GAGGAGATTG AAGTGTCAGA CTCTGGTTTG CTTGATGAGT ATGTGGCTCT GTACACGTAT
    GAGAGTCCGG AGTCAGGAGA CCTGACGTTC AGTGCAGAGG ATGTGGTTCT GGTTACAGAG
    AAAGAAGGAG AGTGGTGGAG AGGCTGTATT GGTGACCAGA CCGGCCTTTT CCCCTCCAAC
    TATGTTAAAC CCAAAGAACC TGATACTGCC AGTTCTGCAG TTCCCAGTAA AAAGCCAGAA
    ATTGCTCAGG TGACTACAGT CAGTCCTGCA ACAACACCAG AGCAGCTGAG TCTGACACCT
    GGCCAGCTGA TCGTGGTGCT GCATAAGAAC TCAAACAGCT GGTGGCTTGG TGAACTACAG
    GCACGGGGTA AGAAGCGTAA AAAGGGCTGG TTTCAATCTT CTAATGTCCG ATTACTGGAG
    GCCAACAGCG GCAAAATAAC ACCTGCACCT CAACCATTGT GTCAGGTTAT TGCAATGTAT
    GACTACAAAG CAGCCAATAA GGACGAGATG AGCTTCCAGA AAGGTCAGCT GATCACCGTA
    CTGAACAAGG ACAACCCTGA CTGGTGGAAA GGAGAGGTCG CAGGGGTCAC AGGGCTGTTT
    CCAACTAATT ATGTGAAGAT GACCACAGAA TGTGATCCAA GCCAGCAATG GTGTGCTGAC
    CTGCTTAGTT TAGACTCTAT GTGCACTGAG GAGAGGAAGA GGCAAGGCTA CATCCATGAG
    CTCATTCAGA CAGAAGAGTC TTACCTGGAG GACCTGGAGC TGGCAGTGGA GGTGTTTTAT
    AAGCCAATGG CAGAGTCGGG ACGGCTGACC GAGGCTGAGA TCAGCATGAT CTTTGTGAAC
    TGGCGTGAAT TGATCATGTG CAGCACCAAA CTCCTAAAAG CTCTGCGTGT GCGTAAGAAG
    ACAGCAGGTG AGCGAATGCC CGTGCAGGTG GTTGGTGATA TCTTGTCCTC TGAGCTCTCT
    CACATGCAGG CTTACATTCG CTTCTGCAGC TGCCAGCTCA ACGCAGCTGC TCTGTTGCAG
    CAGAAAACAG ACAAATCACT CGACTTCAAA CTCTTCCTCA AGAAAATTGC CAGTAATTAC
    CGCTGTAAAG GAATGCCACT GTCCAGCTTT CTCCTGAAGC CCATGCAGAG GATCACACGA
    TACCCACTGC TGATCAAAAA CATTCTGGAA AACACTCCTC CGGCTCACGC TGACCACTCC
    AATCTGCAGG CGGCGCTGGA GCAGGCGGAG GAGCTTTGCT CACAGGTGAA CGAGGCTGTG
    AGGGAGAAAG AGAACTCCGA CCGACTGGAG TGGATCCAGA ACCACGTGTT GTGTGACGGA
    GTCATTGAGC ACCTTGTTTT TAACTCTCTG ACGAACTGCC TGGGCCCTCG AAAACTGCTG
    CACAGCGGCA AACTGCATAA GACCAAGAGC AGCAAAGAGC TCTGGGCCCT GCTGTTCAAT
    GACTTCCTGC TCCTCACATA CACCTCCAAA CAGTTCTCAT CCAGCCCAGA CAAACTCTTC
    AATCCCAACT CCAACACACA GTACAAGATG TACAAGACGC CAGTGTTTCT GAATGAGGTC
    TTGGTGAAAA TGCCCTCTGA CCCGTCCAGC GATGACCCAG TTTTCCACAT TTCACACATT
    GATCGTGTTT ACACACTCAA GACTGACACT ATCAATGAGA GGACGACGTG GGTGCAGAAG
    ATCAAAGCAG CATCTGATCA CTTTATAGAG ACGGAGAAGC TGAAGAGAGA GAAGGCGTAT
    CAAGCTCGCT CTCAGAAGAA CAGTGGCATC GGTCGACTGC TGGTAACAGT GCTGGAAGCC
    ACAGAGCTGA AGCCCTGCAA ACCCAACGGG AAGAGTAACC CATACTGTGA ACTAACCATG
    GGCGCCCAGT GCTACACGTC CCGGCATCAG CCCGACACGC TCAACCCAAA ATGGAGCTTC
    AACTGCCAGT TCTTCATCAA AGACTTGTAC CAGGATGTCC TGTGCCTCAC TGTCTTTGAG
    AGGGACCAGT TCTCTCCTGA TGATTTTCTA GGTCGAACTG AAGTTCCTGT AGCGACAATA
    AAAAAAGATC AAGATGGTAA AGGGCCACTG GTTCGACGTC TGCTGCTTCA TGAAGTCCCG
    ACTGGAGAGG TTAAAGTGAG GCTTGATCTG CAGCTCTATG AACAAACACC TCACGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44866
    Clone ID Related Accession (Same CDS sequence) XM_009300809.3 , XM_009300809.2
    Accession Version XM_009300809.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4920bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product intersectin 2a isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300809.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATGGTG GCTTTTCTAT TTGGGCTATA ACTCCAGAAG AGAGGGGGAA ACATGACAAA 
    CAGTTTGACT CGCTGGCTCC CACCCTGGGA TATTTGTCGG GAGATCAAGC AAGAAAGTTC
    TTCCTCCAAT CTGGTCTTCC CACATCGGTC CTGGCAGACA TCTGGGCTCT GGCGGACATA
    GGAAAGGATG GGAAGATGGA CAGATTGGAG TTCTCTATTG CAATGAAACT AATTAAACTG
    CAGCTGCAGG GTCAGCCACT TCCGTCCAGC CTGCCGATCA TCATGAAGCA GACGCCCACC
    CCTGTGATGA CGTCATCGTC ACGCTTCGGT ATGGGCTCCA TGCCAAATCT CTCCGCCATG
    TCGGTGATGC CCATCCTAAC ACCCATCCCC ACATGCCCTG TCATGGCCCC TTTAGTTCCT
    GTGCCCACCC TGATATCTGG GCCACTGCCT CAGATGCCCA ACTCTATCAG TGTGCCAGCC
    TTACCTAACG GCAATGCAAC TCTCCTCACA CCAACACCAG CATTCCCAGT CTCCAGTGGT
    TTGACTAAAT CTCACTCTCT CCTGGACCTT GGCTCTAGCA GTTCTAACTC GTCCTCCACC
    ACCTCCCTTG CGAGTAACTC CCCGAAAACG AGTAGTTCTG ATTGGGCAGT GCCTCAGTCA
    TCCCGTCTGA AGTACCGACA ACTGTTTAAC AGCCTGGACA AGCTAATGAG TGGATACCTG
    TCAGGTCCCC AGGTCAGAAA TGCCCTCACA GCTTCAAACC TCACACAAAC ACAGCTTGCC
    ACTATATGGT TTCTGGCTGA TGTGGATCGT GATGGGCAGT TGAGAGCAGA AGAGTTTATT
    TTAGCGATGC ATCTGGTTGA AATGGCCAAG ACAGGGCGGC CTTTACCTCT CACTTTACCT
    CCCGACCTTG TGCCTCCTTC ACTCAGAACA ACTAAGTCAT GTGACCTGCT AAACGGACCT
    GTTCCTCTGA TTAGCACTGA GCTGATTGAG CCAGAACTGC CACAGAAAAA CAAGAGCAAT
    GTTTCATATG AAGACAAACT GAGGGAAAAT TTCCAGAGGG GAAACGCAGA GCTGGAGAAG
    CGTCGCCTGG CTCTTCAGGA GCAGCAGAGG AGAGAGGAGG AGCGCAGGAG AGAAGAGGAG
    AGGCGAGAGG AGGAGAAGAG ACAGCAGAAG GAAAGAGAGG AGCGAGAGAG GAGAGAGAGA
    GAAGCGCGAG AAATGGAGAT CAGGAGACAG AGAGAGGAGG AGCGCAGGAT GGAGAGACAG
    AGAGAGCTGG AGCGACAGAG AGAGGAGGAA CGACTGAAAG AATTAGAGCG GCGAGAGGAA
    GCTGAGCGAC AAAGACGGAG GGAATGGGAG AAGAGCAAAC GTGTGGAGCT TCAGACTCAG
    AAAGAAAAGG AGCAGAATGA CATACAGAGA CTAAAGGACA GGAAGAGGAG TCTTGAGATT
    GAGCTGGAGG CCGTGGGTAA CAAGCATAAG CAGATCTCCA GTCAGCTGCG TGATGCTAAG
    AGTAAGAGAC AGGGGCAGTG GGCCGAGTTG GAGCTGATCA ACCAGAGGAG AGACGTCTGC
    ATTACTGATA TCAACTCACT GCAGTTACAG TTTGATGAGA TGCAGCGGCA GTTGAATCAG
    CTGGGCCCTG AAAAGCAGCG ACTGGCTGAC AGACTTGTAC ATTTTACCCA AAACATCTCC
    AGTCCTTTGA TAAACGATGT GAAACAGAGT GTGTGCGAGA AAGATCGGAG CTGTCGGAAG
    CTTAAGGAGC AGCTGGATGT TTTGGAGAGA GAAACCACGG CCAAACTCTC ACAAATGGAG
    CAGTACAACA AAGAAATCAA GGAATTGAGA CAGAAGCAGA GCCAGCAGCA GGACGTCCTG
    GAGCAGCTCA GGAGGGTCAG ATCTGACAAA CTCAAAGAGC TCCAGAGACA CAGGAAAGAG
    GAAGAGGAGA GAAAGAGGAA GAAAGAAGAG GAGGCCGCCA GACAGGCCCA GTTGGAGCAG
    GAGCGGCTGG AGCAGGAGGA GGCTGAGCGG CAAAGGAAAC TTGCACAGGA ACGAGAGGCC
    AAACTCAGGG AAGAGCAGCA GCGGCAGGCT CTGGCCAGAC TGCTGGAGGC TCAGGAGCAG
    GCCAGAGCCA AAGAGGAGAA GAGGAGAGCT GAGGAGAAGG AGAGGAATGA GCTAGATGTA
    CACACTCAAT CCAGTCTGCC TTTCAACAGC ACAGAGAACA ACATTTTGCA GCCAGTAGTG
    ATCCCTGAAG TCACGTGTAG TTCTCTGACC ACCTACAGAG CTCTCTATCC CTTCACTGCA
    CGCAACTCAG ACGAGCTCAC GCTGGAGGCA GACTGTTTCA TCCAGGTGGA TGAATCGACA
    GTCAGAGAGA CCGGCTGGCT GTATGGCAGT TACAGTGGAA ACAGAGGCTG GTTTCCAGAG
    AGCTATGCTG AGAGATGCAG TAAAGACAGT CAGACTCAAC CGACCGCAGC TGAAACTGAG
    TCCACTGCGC CCTCTGCCAA CTACCCTGGA ATTCCTCGAG TGGACATAGA AGGACCAACA
    CCGACACACA CCCCAGTATC CACAAACACA CAGCACTCAC AGGCTGTTGC TCTGTGTGAG
    TGGAGCGCTA AGACTGACAG TCACCTGGGC TTCTGCAAGG ACGACATTAT TACAGTTCTG
    GAGAAGCAGG AGAACTGGTG GTACGGAGAA CTCAAGGAGA AACGGGGCTG GTTCCCCTGT
    TCACATGTCT CTGTGGTCAC CACCAATAAT ACACAGAGCG AGCCTCTGTA TTCTAGCGTG
    GAGGAGATTG AAGTGTCAGA CTCTGGTTTG CTTGATGAGT ATGTGGCTCT GTACACGTAT
    GAGAGTCCGG AGTCAGGAGA CCTGACGTTC AGTGCAGAGG ATGTGGTTCT GGTTACAGAG
    AAAGAAGGAG AGTGGTGGAG AGGCTGTATT GGTGACCAGA CCGGCCTTTT CCCCTCCAAC
    TATGTTAAAC CCAAAGAACC TGATACTGCC AGTTCTGCAG TTCCCAGTAA AAAGCCAGAA
    ATTGCTCAGG TGACTACAGT CAGTCCTGCA ACAACACCAG AGCAGCTGAG TCTGACACCT
    GGCCAGCTGA TCGTGGTGCT GCATAAGAAC TCAAACAGCT GGTGGCTTGG TGAACTACAG
    GCACGGGGTA AGAAGCGTAA AAAGGGCTGG TTTCAATCTT CTAATGTCCG ATTACTGGAG
    GCCAACAGCG GCAAAATAAC ACCTGCACCT CAACCATTGT GTCAGGTTAT TGCAATGTAT
    GACTACAAAG CAGCCAATAA GGACGAGATG AGCTTCCAGA AAGGTCAGCT GATCACCGTA
    CTGAACAAGG ACAACCCTGA CTGGTGGAAA GGAGAGGTCG CAGGGGTCAC AGGGCTGTTT
    CCAACTAATT ATGTGAAGAT GACCACAGAA TGTGATCCAA GCCAGCAATG GTGTGCTGAC
    CTGCTTAGTT TAGACTCTAT GTGCACTGAG GAGAGGAAGA GGCAAGGCTA CATCCATGAG
    CTCATTCAGA CAGAAGAGTC TTACCTGGAG GACCTGGAGC TGGCAGTGGA GGTGTTTTAT
    AAGCCAATGG CAGAGTCGGG ACGGCTGACC GAGGCTGAGA TCAGCATGAT CTTTGTGAAC
    TGGCGTGAAT TGATCATGTG CAGCACCAAA CTCCTAAAAG CTCTGCGTGT GCGTAAGAAG
    ACAGCAGGTG AGCGAATGCC CGTGCAGGTG GTTGGTGATA TCTTGTCCTC TGAGCTCTCT
    CACATGCAGG CTTACATTCG CTTCTGCAGC TGCCAGCTCA ACGCAGCTGC TCTGTTGCAG
    CAGAAAACAG ACAAATCACT CGACTTCAAA CTCTTCCTCA AGAAAATTGC CAGTAATTAC
    CGCTGTAAAG GAATGCCACT GTCCAGCTTT CTCCTGAAGC CCATGCAGAG GATCACACGA
    TACCCACTGC TGATCAAAAA CATTCTGGAA AACACTCCTC CGGCTCACGC TGACCACTCC
    AATCTGCAGG CGGCGCTGGA GCAGGCGGAG GAGCTTTGCT CACAGGTGAA CGAGGCTGTG
    AGGGAGAAAG AGAACTCCGA CCGACTGGAG TGGATCCAGA ACCACGTGTT GTGTGACGGA
    GTCATTGAGC ACCTTGTTTT TAACTCTCTG ACGAACTGCC TGGGCCCTCG AAAACTGCTG
    CACAGCGGCA AACTGCATAA GACCAAGAGC AGCAAAGAGC TCTGGGCCCT GCTGTTCAAT
    GACTTCCTGC TCCTCACATA CACCTCCAAA CAGTTCTCAT CCAGCCCAGA CAAACTCTTC
    AATCCCAACT CCAACACACA GTACAAGATG TACAAGACGC CAGTGTTTCT GAATGAGGTC
    TTGGTGAAAA TGCCCTCTGA CCCGTCCAGC GATGACCCAG TTTTCCACAT TTCACACATT
    GATCGTGTTT ACACACTCAA GACTGACACT ATCAATGAGA GGACGACGTG GGTGCAGAAG
    ATCAAAGCAG CATCTGATCA CTTTATAGAG ACGGAGAAGC TGAAGAGAGA GAAGGCGTAT
    CAAGCTCGCT CTCAGAAGAA CAGTGGCATC GGTCGACTGC TGGTAACAGT GCTGGAAGCC
    ACAGAGCTGA AGCCCTGCAA ACCCAACGGG AAGAGTAACC CATACTGTGA ACTAACCATG
    GGCGCCCAGT GCTACACGTC CCGGCATCAG CCCGACACGC TCAACCCAAA ATGGAGCTTC
    AACTGCCAGT TCTTCATCAA AGACTTGTAC CAGGATGTCC TGTGCCTCAC TGTCTTTGAG
    AGGGACCAGT TCTCTCCTGA TGATTTTCTA GGTCGAACTG AAGTTCCTGT AGCGACAATA
    AAAAAAGATC AAGATGGTAA AGGGCCACTG GTTCGACGTC TGCTGCTTCA TGAAGTCCCG
    ACTGGAGAGG TTAAAGTGAG GCTTGATCTG CAGCTCTATG AACAAACACC TCACGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299084.1
    CDS205..5124
    Translation

    Target ORF information:

    RefSeq Version XM_009300809.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio intersectin 2a (itsn2a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300809.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGAATGGTG GCTTTTCTAT TTGGGCTATA ACTCCAGAAG AGAGGGGGAA ACATGACAAA 
    CAGTTTGACT CGCTGGCTCC CACCCTGGGA TATTTGTCGG GAGATCAAGC AAGAAAGTTC
    TTCCTCCAAT CTGGTCTTCC CACATCGGTC CTGGCAGACA TCTGGGCTCT GGCGGACATA
    GGAAAGGATG GGAAGATGGA CAGATTGGAG TTCTCTATTG CAATGAAACT AATTAAACTG
    CAGCTGCAGG GTCAGCCACT TCCGTCCAGC CTGCCGATCA TCATGAAGCA GACGCCCACC
    CCTGTGATGA CGTCATCGTC ACGCTTCGGT ATGGGCTCCA TGCCAAATCT CTCCGCCATG
    TCGGTGATGC CCATCCTAAC ACCCATCCCC ACATGCCCTG TCATGGCCCC TTTAGTTCCT
    GTGCCCACCC TGATATCTGG GCCACTGCCT CAGATGCCCA ACTCTATCAG TGTGCCAGCC
    TTACCTAACG GCAATGCAAC TCTCCTCACA CCAACACCAG CATTCCCAGT CTCCAGTGGT
    TTGACTAAAT CTCACTCTCT CCTGGACCTT GGCTCTAGCA GTTCTAACTC GTCCTCCACC
    ACCTCCCTTG CGAGTAACTC CCCGAAAACG AGTAGTTCTG ATTGGGCAGT GCCTCAGTCA
    TCCCGTCTGA AGTACCGACA ACTGTTTAAC AGCCTGGACA AGCTAATGAG TGGATACCTG
    TCAGGTCCCC AGGTCAGAAA TGCCCTCACA GCTTCAAACC TCACACAAAC ACAGCTTGCC
    ACTATATGGT TTCTGGCTGA TGTGGATCGT GATGGGCAGT TGAGAGCAGA AGAGTTTATT
    TTAGCGATGC ATCTGGTTGA AATGGCCAAG ACAGGGCGGC CTTTACCTCT CACTTTACCT
    CCCGACCTTG TGCCTCCTTC ACTCAGAACA ACTAAGTCAT GTGACCTGCT AAACGGACCT
    GTTCCTCTGA TTAGCACTGA GCTGATTGAG CCAGAACTGC CACAGAAAAA CAAGAGCAAT
    GTTTCATATG AAGACAAACT GAGGGAAAAT TTCCAGAGGG GAAACGCAGA GCTGGAGAAG
    CGTCGCCTGG CTCTTCAGGA GCAGCAGAGG AGAGAGGAGG AGCGCAGGAG AGAAGAGGAG
    AGGCGAGAGG AGGAGAAGAG ACAGCAGAAG GAAAGAGAGG AGCGAGAGAG GAGAGAGAGA
    GAAGCGCGAG AAATGGAGAT CAGGAGACAG AGAGAGGAGG AGCGCAGGAT GGAGAGACAG
    AGAGAGCTGG AGCGACAGAG AGAGGAGGAA CGACTGAAAG AATTAGAGCG GCGAGAGGAA
    GCTGAGCGAC AAAGACGGAG GGAATGGGAG AAGAGCAAAC GTGTGGAGCT TCAGACTCAG
    AAAGAAAAGG AGCAGAATGA CATACAGAGA CTAAAGGACA GGAAGAGGAG TCTTGAGATT
    GAGCTGGAGG CCGTGGGTAA CAAGCATAAG CAGATCTCCA GTCAGCTGCG TGATGCTAAG
    AGTAAGAGAC AGGGGCAGTG GGCCGAGTTG GAGCTGATCA ACCAGAGGAG AGACGTCTGC
    ATTACTGATA TCAACTCACT GCAGTTACAG TTTGATGAGA TGCAGCGGCA GTTGAATCAG
    CTGGGCCCTG AAAAGCAGCG ACTGGCTGAC AGACTTGTAC ATTTTACCCA AAACATCTCC
    AGTCCTTTGA TAAACGATGT GAAACAGAGT GTGTGCGAGA AAGATCGGAG CTGTCGGAAG
    CTTAAGGAGC AGCTGGATGT TTTGGAGAGA GAAACCACGG CCAAACTCTC ACAAATGGAG
    CAGTACAACA AAGAAATCAA GGAATTGAGA CAGAAGCAGA GCCAGCAGCA GGACGTCCTG
    GAGCAGCTCA GGAGGGTCAG ATCTGACAAA CTCAAAGAGC TCCAGAGACA CAGGAAAGAG
    GAAGAGGAGA GAAAGAGGAA GAAAGAAGAG GAGGCCGCCA GACAGGCCCA GTTGGAGCAG
    GAGCGGCTGG AGCAGGAGGA GGCTGAGCGG CAAAGGAAAC TTGCACAGGA ACGAGAGGCC
    AAACTCAGGG AAGAGCAGCA GCGGCAGGCT CTGGCCAGAC TGCTGGAGGC TCAGGAGCAG
    GCCAGAGCCA AAGAGGAGAA GAGGAGAGCT GAGGAGAAGG AGAGGAATGA GCTAGATGTA
    CACACTCAAT CCAGTCTGCC TTTCAACAGC ACAGAGAACA ACATTTTGCA GCCAGTAGTG
    ATCCCTGAAG TCACGTGTAG TTCTCTGACC ACCTACAGAG CTCTCTATCC CTTCACTGCA
    CGCAACTCAG ACGAGCTCAC GCTGGAGGCA GACTGTTTCA TCCAGGTGGA TGAATCGACA
    GTCAGAGAGA CCGGCTGGCT GTATGGCAGT TACAGTGGAA ACAGAGGCTG GTTTCCAGAG
    AGCTATGCTG AGAGATGCAG TAAAGACAGT CAGACTCAAC CGACCGCAGC TGAAACTGAG
    TCCACTGCGC CCTCTGCCAA CTACCCTGGA ATTCCTCGAG TGGACATAGA AGGACCAACA
    CCGACACACA CCCCAGTATC CACAAACACA CAGCACTCAC AGGCTGTTGC TCTGTGTGAG
    TGGAGCGCTA AGACTGACAG TCACCTGGGC TTCTGCAAGG ACGACATTAT TACAGTTCTG
    GAGAAGCAGG AGAACTGGTG GTACGGAGAA CTCAAGGAGA AACGGGGCTG GTTCCCCTGT
    TCACATGTCT CTGTGGTCAC CACCAATAAT ACACAGAGCG AGCCTCTGTA TTCTAGCGTG
    GAGGAGATTG AAGTGTCAGA CTCTGGTTTG CTTGATGAGT ATGTGGCTCT GTACACGTAT
    GAGAGTCCGG AGTCAGGAGA CCTGACGTTC AGTGCAGAGG ATGTGGTTCT GGTTACAGAG
    AAAGAAGGAG AGTGGTGGAG AGGCTGTATT GGTGACCAGA CCGGCCTTTT CCCCTCCAAC
    TATGTTAAAC CCAAAGAACC TGATACTGCC AGTTCTGCAG TTCCCAGTAA AAAGCCAGAA
    ATTGCTCAGG TGACTACAGT CAGTCCTGCA ACAACACCAG AGCAGCTGAG TCTGACACCT
    GGCCAGCTGA TCGTGGTGCT GCATAAGAAC TCAAACAGCT GGTGGCTTGG TGAACTACAG
    GCACGGGGTA AGAAGCGTAA AAAGGGCTGG TTTCAATCTT CTAATGTCCG ATTACTGGAG
    GCCAACAGCG GCAAAATAAC ACCTGCACCT CAACCATTGT GTCAGGTTAT TGCAATGTAT
    GACTACAAAG CAGCCAATAA GGACGAGATG AGCTTCCAGA AAGGTCAGCT GATCACCGTA
    CTGAACAAGG ACAACCCTGA CTGGTGGAAA GGAGAGGTCG CAGGGGTCAC AGGGCTGTTT
    CCAACTAATT ATGTGAAGAT GACCACAGAA TGTGATCCAA GCCAGCAATG GTGTGCTGAC
    CTGCTTAGTT TAGACTCTAT GTGCACTGAG GAGAGGAAGA GGCAAGGCTA CATCCATGAG
    CTCATTCAGA CAGAAGAGTC TTACCTGGAG GACCTGGAGC TGGCAGTGGA GGTGTTTTAT
    AAGCCAATGG CAGAGTCGGG ACGGCTGACC GAGGCTGAGA TCAGCATGAT CTTTGTGAAC
    TGGCGTGAAT TGATCATGTG CAGCACCAAA CTCCTAAAAG CTCTGCGTGT GCGTAAGAAG
    ACAGCAGGTG AGCGAATGCC CGTGCAGGTG GTTGGTGATA TCTTGTCCTC TGAGCTCTCT
    CACATGCAGG CTTACATTCG CTTCTGCAGC TGCCAGCTCA ACGCAGCTGC TCTGTTGCAG
    CAGAAAACAG ACAAATCACT CGACTTCAAA CTCTTCCTCA AGAAAATTGC CAGTAATTAC
    CGCTGTAAAG GAATGCCACT GTCCAGCTTT CTCCTGAAGC CCATGCAGAG GATCACACGA
    TACCCACTGC TGATCAAAAA CATTCTGGAA AACACTCCTC CGGCTCACGC TGACCACTCC
    AATCTGCAGG CGGCGCTGGA GCAGGCGGAG GAGCTTTGCT CACAGGTGAA CGAGGCTGTG
    AGGGAGAAAG AGAACTCCGA CCGACTGGAG TGGATCCAGA ACCACGTGTT GTGTGACGGA
    GTCATTGAGC ACCTTGTTTT TAACTCTCTG ACGAACTGCC TGGGCCCTCG AAAACTGCTG
    CACAGCGGCA AACTGCATAA GACCAAGAGC AGCAAAGAGC TCTGGGCCCT GCTGTTCAAT
    GACTTCCTGC TCCTCACATA CACCTCCAAA CAGTTCTCAT CCAGCCCAGA CAAACTCTTC
    AATCCCAACT CCAACACACA GTACAAGATG TACAAGACGC CAGTGTTTCT GAATGAGGTC
    TTGGTGAAAA TGCCCTCTGA CCCGTCCAGC GATGACCCAG TTTTCCACAT TTCACACATT
    GATCGTGTTT ACACACTCAA GACTGACACT ATCAATGAGA GGACGACGTG GGTGCAGAAG
    ATCAAAGCAG CATCTGATCA CTTTATAGAG ACGGAGAAGC TGAAGAGAGA GAAGGCGTAT
    CAAGCTCGCT CTCAGAAGAA CAGTGGCATC GGTCGACTGC TGGTAACAGT GCTGGAAGCC
    ACAGAGCTGA AGCCCTGCAA ACCCAACGGG AAGAGTAACC CATACTGTGA ACTAACCATG
    GGCGCCCAGT GCTACACGTC CCGGCATCAG CCCGACACGC TCAACCCAAA ATGGAGCTTC
    AACTGCCAGT TCTTCATCAA AGACTTGTAC CAGGATGTCC TGTGCCTCAC TGTCTTTGAG
    AGGGACCAGT TCTCTCCTGA TGATTTTCTA GGTCGAACTG AAGTTCCTGT AGCGACAATA
    AAAAAAGATC AAGATGGTAA AGGGCCACTG GTTCGACGTC TGCTGCTTCA TGAAGTCCCG
    ACTGGAGAGG TTAAAGTGAG GCTTGATCTG CAGCTCTATG AACAAACACC TCACGTATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44867
    Clone ID Related Accession (Same CDS sequence) XM_009300811.2
    Accession Version XM_009300811.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3441bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product intersectin 2a isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300811.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAATGGTG GCTTTTCTAT TTGGGCTATA ACTCCAGAAG AGAGGGGGAA ACATGACAAA 
    CAGTTTGACT CGCTGGCTCC CACCCTGGGA TATTTGTCGG GAGATCAAGC AAGAAAGTTC
    TTCCTCCAAT CTGGTCTTCC CACATCGGTC CTGGCAGACA TCTGGGCTCT GGCGGACATA
    GGAAAGGATG GGAAGATGGA CAGATTGGAG TTCTCTATTG CAATGAAACT AATTAAACTG
    CAGCTGCAGG GTCAGCCACT TCCGTCCAGC CTGCCGATCA TCATGAAGCA GACGCCCACC
    CCTGTGATGA CGTCATCGTC ACGCTTCGGT ATGGGCTCCA TGCCAAATCT CTCCGCCATG
    TCGGTGATGC CCATCCTAAC ACCCATCCCC ACATGCCCTG TCATGGCCCC TTTAGTTCCT
    GTGCCCACCC TGATATCTGG GCCACTGCCT CAGATGCCCA ACTCTATCAG TGTGCCAGCC
    TTACCTAACG GCAATGCAAC TCTCCTCACA CCAACACCAG CATTCCCAGT CTCCAGTGGT
    TTGACTAAAT CTCACTCTCT CCTGGACCTT GGCTCTAGCA GTTCTAACTC GTCCTCCACC
    ACCTCCCTTG CGAGTAACTC CCCGAAAACG AGTAGTTCTG ATTGGGCAGT GCCTCAGTCA
    TCCCGTCTGA AGTACCGACA ACTGTTTAAC AGCCTGGACA AGCTAATGAG TGGATACCTG
    TCAGGTCCCC AGGTCAGAAA TGCCCTCACA GCTTCAAACC TCACACAAAC ACAGCTTGCC
    ACTATATGGT TTCTGGCTGA TGTGGATCGT GATGGGCAGT TGAGAGCAGA AGAGTTTATT
    TTAGCGATGC ATCTGGTTGA AATGGCCAAG ACAGGGCGGC CTTTACCTCT CACTTTACCT
    CCCGACCTTG TGCCTCCTTC ACTCAGAACA ACTAAGTCAT GTGACCTGCT AAACGGACCT
    GTTCCTCTGA TTAGCACTGA GCTGATTGAG CCAGAACTGC CACAGAAAAA CAAGAGCAAT
    GTTTCATATG AAGACAAACT GAGGGAAAAT TTCCAGAGGG GAAACGCAGA GCTGGAGAAG
    CGTCGCCTGG CTCTTCAGGA GCAGCAGAGG AGAGAGGAGG AGCGCAGGAG AGAAGAGGAG
    AGGCGAGAGG AGGAGAAGAG ACAGCAGAAG GAAAGAGAGG AGCGAGAGAG GAGAGAGAGA
    GAAGCGCGAG AAATGGAGAT CAGGAGACAG AGAGAGGAGG AGCGCAGGAT GGAGAGACAG
    AGAGAGCTGG AGCGACAGAG AGAGGAGGAA CGACTGAAAG AATTAGAGCG GCGAGAGGCA
    AGTAGAGCAA GCAAGGACAT ACAGAGGGAA GCTGAGCGAC AAAGACGGAG GGAATGGGAG
    AAGAGCAAAC GTGTGGAGCT TCAGACTCAG AAAGAAAAGG AGCAGAATGA CATACAGAGA
    CTAAAGGACA GGAAGAGGAG TCTTGAGATT GAGCTGGAGG CCGTGGGTAA CAAGCATAAG
    CAGATCTCCA GTCAGCTGCG TGATGCTAAG AGTAAGAGAC AGGGGCAGTG GGCCGAGTTG
    GAGCTGATCA ACCAGAGGAG AGACGTCTGC ATTACTGATA TCAACTCACT GCAGTTACAG
    TTTGATGAGA TGCAGCGGCA GTTGAATCAG CTGGGCCCTG AAAAGCAGCG ACTGGCTGAC
    AGACTTGTAC ATTTTACCCA AAACATCTCC AGTCCTTTGA TAAACGATGT GAAACAGAGT
    GTGTGCGAGA AAGATCGGAG CTGTCGGAAG CTTAAGGAGC AGCTGGATGT TTTGGAGAGA
    GAAACCACGG CCAAACTCTC ACAAATGGAG CAGTACAACA AAGAAATCAA GGAATTGAGA
    CAGAAGCAGA GCCAGCAGCA GGACGTCCTG GAGCAGCTCA GGAGGGTCAG ATCTGACAAA
    CTCAAAGAGC TCCAGAGACA CAGGAAAGAG GAAGAGGAGA GAAAGAGGAA GAAAGAAGAG
    GAGGCCGCCA GACAGGCCCA GTTGGAGCAG GAGCGGCTGG AGCAGGAGGA GGCTGAGCGG
    CAAAGGAAAC TTGCACAGGA ACGAGAGGCC AAACTCAGGG AAGAGCAGCA GCGGCAGGCT
    CTGGCCAGAC TGCTGGAGGC TCAGGAGCAG GCCAGAGCCA AAGAGGAGAA GAGGAGAGCT
    GAGGAGAAGG AGAGGAATGA GCTAGATGTA CACACTCAAT CCAGTCTGCC TTTCAACAGC
    ACAGAGAACA ACATTTTGCA GCCAGTAGTG ATCCCTGAAG TCACGTGTAG TTCTCTGACC
    ACCTACAGAG CTCTCTATCC CTTCACTGCA CGCAACTCAG ACGAGCTCAC GCTGGAGGCA
    GACTGTTTCA TCCAGGTGGA TGAATCGACA GTCAGAGAGA CCGGCTGGCT GTATGGCAGT
    TACAGTGGAA ACAGAGGCTG GTTTCCAGAG AGCTATGCTG AGAGATGCAG TAAAGACAGT
    CAGACTCAAC CGACCGCAGC TGAAACTGAG TCCACTGCGC CCTCTGCCAA CTACCCTGGA
    ATTCCTCGAG TGGACATAGA AGGACCAACA CCGACACACA CCCCAGTATC CACAAACACA
    CAGCACTCAC AGGCTGTTGC TCTGTGTGAG TGGAGCGCTA AGACTGACAG TCACCTGGGC
    TTCTGCAAGG ACGACATTAT TACAGTTCTG GAGAAGCAGG AGAACTGGTG GTACGGAGAA
    CTCAAGGAGA AACGGGGCTG GTTCCCCTGT TCACATGTCT CTGTGGTCAC CACCAATAAT
    ACACAGAGCG AGCCTCTGTA TTCTAGCGTG GAGGAGATTG AAGTGTCAGA CTCTGGTTTG
    CTTGATGAGT ATGTGGCTCT GTACACGTAT GAGAGTCCGG AGTCAGGAGA CCTGACGTTC
    AGTGCAGAGG ATGTGGTTCT GGTTACAGAG AAAGAAGGAG AGTGGTGGAG AGGCTGTATT
    GGTGACCAGA CCGGCCTTTT CCCCTCCAAC TATGTTAAAC CCAAAGAACC TGATACTGCC
    AGTTCTGCAG TTCCCAGTAA AAAGCCAGAA ATTGCTCAGG TGACTACAGT CAGTCCTGCA
    ACAACACCAG AGCAGCTGAG TCTGACACCT GGCCAGCTGA TCGTGGTGCT GCATAAGAAC
    TCAAACAGCT GGTGGCTTGG TGAACTACAG GCACGGGGTA AGAAGCGTAA AAAGGGCTGG
    TTTCAATCTT CTAATGTCCG ATTACTGGAG GCCAACAGCG GCAAAATAAC ACCTGCACCT
    CAACCATTGT GTCAGGTTAT TGCAATGTAT GACTACAAAG CAGCCAATAA GGACGAGATG
    AGCTTCCAGA AAGGTCAGCT GATCACCGTA CTGAACAAGG ACAACCCTGA CTGGTGGAAA
    GGAGAGGTCG CAGGGGTCAC AGGGCTGTTT CCAACTAATT ATGTGAAGAT GACCACAGAA
    TGTGATCCAA GCCAGCAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009299086.1
    CDS205..3645
    Translation

    Target ORF information:

    RefSeq Version XM_009300811.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio intersectin 2a (itsn2a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009300811.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAATGGTG GCTTTTCTAT TTGGGCTATA ACTCCAGAAG AGAGGGGGAA ACATGACAAA 
    CAGTTTGACT CGCTGGCTCC CACCCTGGGA TATTTGTCGG GAGATCAAGC AAGAAAGTTC
    TTCCTCCAAT CTGGTCTTCC CACATCGGTC CTGGCAGACA TCTGGGCTCT GGCGGACATA
    GGAAAGGATG GGAAGATGGA CAGATTGGAG TTCTCTATTG CAATGAAACT AATTAAACTG
    CAGCTGCAGG GTCAGCCACT TCCGTCCAGC CTGCCGATCA TCATGAAGCA GACGCCCACC
    CCTGTGATGA CGTCATCGTC ACGCTTCGGT ATGGGCTCCA TGCCAAATCT CTCCGCCATG
    TCGGTGATGC CCATCCTAAC ACCCATCCCC ACATGCCCTG TCATGGCCCC TTTAGTTCCT
    GTGCCCACCC TGATATCTGG GCCACTGCCT CAGATGCCCA ACTCTATCAG TGTGCCAGCC
    TTACCTAACG GCAATGCAAC TCTCCTCACA CCAACACCAG CATTCCCAGT CTCCAGTGGT
    TTGACTAAAT CTCACTCTCT CCTGGACCTT GGCTCTAGCA GTTCTAACTC GTCCTCCACC
    ACCTCCCTTG CGAGTAACTC CCCGAAAACG AGTAGTTCTG ATTGGGCAGT GCCTCAGTCA
    TCCCGTCTGA AGTACCGACA ACTGTTTAAC AGCCTGGACA AGCTAATGAG TGGATACCTG
    TCAGGTCCCC AGGTCAGAAA TGCCCTCACA GCTTCAAACC TCACACAAAC ACAGCTTGCC
    ACTATATGGT TTCTGGCTGA TGTGGATCGT GATGGGCAGT TGAGAGCAGA AGAGTTTATT
    TTAGCGATGC ATCTGGTTGA AATGGCCAAG ACAGGGCGGC CTTTACCTCT CACTTTACCT
    CCCGACCTTG TGCCTCCTTC ACTCAGAACA ACTAAGTCAT GTGACCTGCT AAACGGACCT
    GTTCCTCTGA TTAGCACTGA GCTGATTGAG CCAGAACTGC CACAGAAAAA CAAGAGCAAT
    GTTTCATATG AAGACAAACT GAGGGAAAAT TTCCAGAGGG GAAACGCAGA GCTGGAGAAG
    CGTCGCCTGG CTCTTCAGGA GCAGCAGAGG AGAGAGGAGG AGCGCAGGAG AGAAGAGGAG
    AGGCGAGAGG AGGAGAAGAG ACAGCAGAAG GAAAGAGAGG AGCGAGAGAG GAGAGAGAGA
    GAAGCGCGAG AAATGGAGAT CAGGAGACAG AGAGAGGAGG AGCGCAGGAT GGAGAGACAG
    AGAGAGCTGG AGCGACAGAG AGAGGAGGAA CGACTGAAAG AATTAGAGCG GCGAGAGGCA
    AGTAGAGCAA GCAAGGACAT ACAGAGGGAA GCTGAGCGAC AAAGACGGAG GGAATGGGAG
    AAGAGCAAAC GTGTGGAGCT TCAGACTCAG AAAGAAAAGG AGCAGAATGA CATACAGAGA
    CTAAAGGACA GGAAGAGGAG TCTTGAGATT GAGCTGGAGG CCGTGGGTAA CAAGCATAAG
    CAGATCTCCA GTCAGCTGCG TGATGCTAAG AGTAAGAGAC AGGGGCAGTG GGCCGAGTTG
    GAGCTGATCA ACCAGAGGAG AGACGTCTGC ATTACTGATA TCAACTCACT GCAGTTACAG
    TTTGATGAGA TGCAGCGGCA GTTGAATCAG CTGGGCCCTG AAAAGCAGCG ACTGGCTGAC
    AGACTTGTAC ATTTTACCCA AAACATCTCC AGTCCTTTGA TAAACGATGT GAAACAGAGT
    GTGTGCGAGA AAGATCGGAG CTGTCGGAAG CTTAAGGAGC AGCTGGATGT TTTGGAGAGA
    GAAACCACGG CCAAACTCTC ACAAATGGAG CAGTACAACA AAGAAATCAA GGAATTGAGA
    CAGAAGCAGA GCCAGCAGCA GGACGTCCTG GAGCAGCTCA GGAGGGTCAG ATCTGACAAA
    CTCAAAGAGC TCCAGAGACA CAGGAAAGAG GAAGAGGAGA GAAAGAGGAA GAAAGAAGAG
    GAGGCCGCCA GACAGGCCCA GTTGGAGCAG GAGCGGCTGG AGCAGGAGGA GGCTGAGCGG
    CAAAGGAAAC TTGCACAGGA ACGAGAGGCC AAACTCAGGG AAGAGCAGCA GCGGCAGGCT
    CTGGCCAGAC TGCTGGAGGC TCAGGAGCAG GCCAGAGCCA AAGAGGAGAA GAGGAGAGCT
    GAGGAGAAGG AGAGGAATGA GCTAGATGTA CACACTCAAT CCAGTCTGCC TTTCAACAGC
    ACAGAGAACA ACATTTTGCA GCCAGTAGTG ATCCCTGAAG TCACGTGTAG TTCTCTGACC
    ACCTACAGAG CTCTCTATCC CTTCACTGCA CGCAACTCAG ACGAGCTCAC GCTGGAGGCA
    GACTGTTTCA TCCAGGTGGA TGAATCGACA GTCAGAGAGA CCGGCTGGCT GTATGGCAGT
    TACAGTGGAA ACAGAGGCTG GTTTCCAGAG AGCTATGCTG AGAGATGCAG TAAAGACAGT
    CAGACTCAAC CGACCGCAGC TGAAACTGAG TCCACTGCGC CCTCTGCCAA CTACCCTGGA
    ATTCCTCGAG TGGACATAGA AGGACCAACA CCGACACACA CCCCAGTATC CACAAACACA
    CAGCACTCAC AGGCTGTTGC TCTGTGTGAG TGGAGCGCTA AGACTGACAG TCACCTGGGC
    TTCTGCAAGG ACGACATTAT TACAGTTCTG GAGAAGCAGG AGAACTGGTG GTACGGAGAA
    CTCAAGGAGA AACGGGGCTG GTTCCCCTGT TCACATGTCT CTGTGGTCAC CACCAATAAT
    ACACAGAGCG AGCCTCTGTA TTCTAGCGTG GAGGAGATTG AAGTGTCAGA CTCTGGTTTG
    CTTGATGAGT ATGTGGCTCT GTACACGTAT GAGAGTCCGG AGTCAGGAGA CCTGACGTTC
    AGTGCAGAGG ATGTGGTTCT GGTTACAGAG AAAGAAGGAG AGTGGTGGAG AGGCTGTATT
    GGTGACCAGA CCGGCCTTTT CCCCTCCAAC TATGTTAAAC CCAAAGAACC TGATACTGCC
    AGTTCTGCAG TTCCCAGTAA AAAGCCAGAA ATTGCTCAGG TGACTACAGT CAGTCCTGCA
    ACAACACCAG AGCAGCTGAG TCTGACACCT GGCCAGCTGA TCGTGGTGCT GCATAAGAAC
    TCAAACAGCT GGTGGCTTGG TGAACTACAG GCACGGGGTA AGAAGCGTAA AAAGGGCTGG
    TTTCAATCTT CTAATGTCCG ATTACTGGAG GCCAACAGCG GCAAAATAAC ACCTGCACCT
    CAACCATTGT GTCAGGTTAT TGCAATGTAT GACTACAAAG CAGCCAATAA GGACGAGATG
    AGCTTCCAGA AAGGTCAGCT GATCACCGTA CTGAACAAGG ACAACCCTGA CTGGTGGAAA
    GGAGAGGTCG CAGGGGTCAC AGGGCTGTTT CCAACTAATT ATGTGAAGAT GACCACAGAA
    TGTGATCCAA GCCAGCAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.