mcm6 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	mcm6
Entrez Gene ID	566409
Full Name	minichromosome maintenance complex component 6
Synonyms	MCM6z,cb800,im:7140796,wu:fb30d05,wu:fc26f09
General protein information	Preferred Names minichromosome maintenance complex component 6 Names DNA replication licensing factor MCM6 MCM6 minichromosome maintenance deficient 6, mitotin zygotic Mcm6 protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001082849.1	NP_001076318.1	DNA replication licensing factor MCM6

Pathways from BioSystems

Homologies

Homo sapiens (human)	MCM6	NP_005906.2
Pan troglodytes (chimpanzee)	MCM6	XP_001154732.1
Anopheles gambiae (African malaria mosquito)	MCM6_ANOGA	XP_319406.3
Xenopus tropicalis (tropical clawed frog)	mcm6.2	NP_989393.1
	MCM6	XP_001096068.1
Bos taurus (cattle)	MCM6	NP_001039699.1
Rattus norvegicus (Norway rat)	Mcm6	NP_058983.1
Canis lupus familiaris (dog)	MCM6	XP_533338.3
Mus musculus (house mouse)	Mcm6	NP_032593.1
Gallus gallus (chicken)	MCM6	NP_001006527.1
Danio rerio (zebrafish)	mcm6	NP_001076318.1
Drosophila melanogaster (fruit fly)	Mcm6	NP_511065.1

Gene Ontology
Bibliography

Related articles in PubMed

Cohesin facilitates zygotic genome activation in zebrafish.
Meier M, Grant J, Dowdle A, Thomas A, Gerton J, Collas P, O'Sullivan JM, Horsfield JA
Development (Cambridge, England)145(1)(2018 Jan)

DHX33 Transcriptionally Controls Genes Involved in the Cell Cycle.
Yuan B, Wang X, Fan C, You J, Liu Y, Weber JD, Zhong H, Zhang Y
Molecular and cellular biology36(23)2903-2917(2016 Dec)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Telomerase Is Essential for Zebrafish Heart Regeneration.
Bednarek D, Gonz?lez-Rosa JM, Guzm?n-Mart?nez G, Guti?rrez-Guti?rrez ?, Aguado T, S?nchez-Ferrer C, Marques IJ, Galardi-Castilla M, de Diego I, G?mez MJ, Cort?s A, Zapata A, Jim?nez-Borreguero LJ, Mercader N, Flores I
Cell reports12(10)1691-703(2015 Sep)

Evolutionary diversification of MCM3 genes in Xenopus laevis and Danio rerio.
Shinya M, Machiki D, Henrich T, Kubota Y, Takisawa H, Mimura S
Cell cycle (Georgetown, Tex.)13(20)3271-81(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mcm6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mcm6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa02728	NM_001082849.1 Latest version!	Danio rerio minichromosome maintenance complex component 6 (mcm6), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mcm6 ( NM_001082849.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001076318.1 Danio rerio minichromosome maintenance complex component 6 (mcm6), mRNA.

Price & Availability↑

CloneID	ODa02728
Clone ID Related Accession (Same CDS sequence)	NM_001082849.1
Accession Version	NM_001082849.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	DNA replication licensing factor MCM6
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC133090.1. On Mar 15, 2007 this sequence version replaced XM_689678.2. ##Evidence-Data-START## Transcript exon combination :: BC133090.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGATCTTA CGGAGACTGG AGTGAATCAG AACGCTGGCC AGGTGATCAA GGACGAGCTG 
GCTGAAAAAT GTCAGAAATT ATTCCAGGCC TTTTTGGAAG AGTTTCAGAA TAGCGATGGT 
GAGGTGAAGT ACCTGCGGGA CGCGGAGGAG CTGATCCGGC CTGAGAGGAA CACACTCGCG 
GTCAGTTTTA CCGACCTTGA GGGATTTAAC CAAGAACTGG CAACCGCCAT CCAGGAGGAA 
TTCTATAGGC TATACCCCTA CTTGTGCCGT GCTGTTCGTA ATTTTGCTCG GGAACATGGA 
GAAGTTCCCA CCTCCAAGGA ATTCTACGTG GCCTTCCAGG ATCTACCAAC CAGACACAAG 
ATTCGTGAGT TGACCACAGT GAAGGTCGGT TCACTGGTGC GTATCAGCGG TCAGGTGGTC 
CGTACTCACC CGGTTCACCC TGAGCTGGTG AGCGGGACCT TTCTGTGTTT GGACTGTCAG 
GGAGTGATAA AAGACGTGGA GCAGCAGTTT AAATACACTC AGCCCAGCAT TTGCCGCAAT 
CCTGTGTGCA ACAACCGCAG ACGCTTTCTC CTAGATACCA ACAAGTCCAA ATTCATTGAC 
TTCCAGAAGT TACGCATCCA AGAGACGCAG GCTGAGCTGC CCCGTGGCTC CATTCCTCGC 
AGTATGGAGG TGATCCTGCG GGCCGAGGCT GTGGAGTCAG CACAGGCCGG GGATAAATGT 
GACTTCATTG GCTCCCTCAT CGTTGTGCCT GATATATCCC AGTTGGCCAC ACCAGGTGTG 
CGCGCTGAGA CCAGCTCTCG TACGGCAGGA GTCCAGGGCT ATGAGAACGA AGGTTTGCGT 
GGGCTTAAAG CGCTGGGCGT CAGGGAACTG TCCTACAAAC TGGCTTTCTT GGCGTGCCAC 
GTTGCCCCCA CCAATCCCAG ATTTGGAGGC AAGGAGATTC GAGATGAGGA GCAAACGGCA 
GAGAGTATCA AGAAACAGAT GTCAGTGCAG GAGTGGGAGA AGGTGTTTGA GATGAGTCAA 
GATAAAAACC TCTACCATAA CCTCTGCACC AGCCTGTTCC CTACTATTCA CGGTAATGAT 
GAGGTAAAGC GAGGAATTCT GTTGATGCTG TTCGGAGGTG TTCCCAAAAC CACCATGGAG 
GGAACGTCTT TGAGAGGAGA CATCAACGTT TGTGTTGTAG GAGATCCAAG CACAGCTAAA 
AGCCAGTTCC TCAAGCATGT GGAGGAGTTC TGTCCTCGGG CCGTCTACAC CAGCGGCAAA 
GCCTCCAGCG CTGCCGGTCT CACAGCTGCT GTGGTGAGAG ATGAGGAGTC ACACGAGTTC 
GTCATTGAAG CCGGAGCTCT CATGCTGGCT GACAACGGTG TTTGCTGCAT TGACGAGTTT 
GATAAGATGG AAACGAGAGA CCAGGTGGCC ATCCATGAAG CCATGGAGCA GCAGACCATC 
TCCATCACCA AAGCAGGAGT CAAGGCCACT CTGAATGCTC GCACATCCAT CTTGGCTGCA 
GCAAATCCAG TGAGCGGCCG TTACGACCGA TCCAAATCTC TCAAACAGAA CGTCAACCTG 
ACGGCACCCA TCATGTCTCG ATTTGACCTA TTCTTCATCC TTGTGGATGA CTGCAATGAG 
GTCACTGATT ATGCTATAGC CAGACGTATA GTGGATCTCC ACTCCAGGAT TGAGAATTCT 
GTCGACCGCG TCTACAGTCT CGATGAGATT CGCAGATACC TGCTGTTTGC TCGCCAGTTC 
AAGCCAAAGA TCTCAAAAGA GTCTGAAGAG TTTATCGTGG AGCAGTACAA GCGACTGAGA 
CAGCGGGACG GGTCCGGAGT CACAAAGTCA GCCTGGAGGA TCACAGTCCG CCAGCTGGAG 
AGCTTGATCC GGCTCTCTGA GAGCATGGCC AGGATGCACT GCTGCGATGA GGTTCAACCA 
AAGCATGTCA AGGAGGCTTT CAGGCTGCTG AACAAGTCTA TTATTCGGGT TGAGACGCCT 
GATGTTAATC TGGATCAAGA GGAAGAGGAG GCTATGGAGG AGGAAGAGGA CAATCAGATG 
AATGGGCACG ATGTACCTAA CGGTGTAAAT GGAGCTGTTA ATGGAGAAGT GAATGGCCAC 
GAGCATACAA ATGGTATCAA CGGTCACGGG GTCAATGGAG AAACTGCAAA ACCCTCACTG 
AGACTGTCGT TTGCTGAGTA TAAACGCATT TCAAACCTGC TGGTGCTGCA TCTGCGTCGT 
GCTGAGGAAG ATGAGGACGA GGCGGCGCTG AAGAAGAGTG AAGTTGTGAA CTGGTACCTG 
AAGGAGATCG AATCAGAGAT TGATTCTGAA ATGGAGCTCA TCAGTAAGAA GACCATGATA 
GAGAAAGTGA TCTACAGACT GGTTCACTAT GATTATATTC TGATTGAGTT AAAACAGTCC 
GGGCTGAAAG GGTCTGCTGA GTCCAGTGAA ACAGAATCGC AGGAGGAAGA CGTTACGCTG 
GTGGTCAATC CCAACTACAC TTTCGAAGAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076318.1
CDS	42..2534
Translation	MDLTETGVNQNAGQVIKDELAEKCQKLFQAFLEEFQNSDGEVKYLRDAEELIRPERNTLAVSFTDLEGFNQELATAIQEEFYRLYPYLCRAVRNFAREHGEVPTSKEFYVAFQDLPTRHKIRELTTVKVGSLVRISGQVVRTHPVHPELVSGTFLCLDCQGVIKDVEQQFKYTQPSICRNPVCNNRRRFLLDTNKSKFIDFQKLRIQETQAELPRGSIPRSMEVILRAEAVESAQAGDKCDFIGSLIVVPDISQLATPGVRAETSSRTAGVQGYENEGLRGLKALGVRELSYKLAFLACHVAPTNPRFGGKEIRDEEQTAESIKKQMSVQEWEKVFEMSQDKNLYHNLCTSLFPTIHGNDEVKRGILLMLFGGVPKTTMEGTSLRGDINVCVVGDPSTAKSQFLKHVEEFCPRAVYTSGKASSAAGLTAAVVRDEESHEFVIEAGALMLADNGVCCIDEFDKMETRDQVAIHEAMEQQTISITKAGVKATLNARTSILAAANPVSGRYDRSKSLKQNVNLTAPIMSRFDLFFILVDDCNEVTDYAIARRIVDLHSRIENSVDRVYSLDEIRRYLLFARQFKPKISKESEEFIVEQYKRLRQRDGSGVTKSAWRITVRQLESLIRLSESMARMHCCDEVQPKHVKEAFRLLNKSIIRVETPDVNLDQEEEEAMEEEEDNQMNGHDVPNGVNGAVNGEVNGHEHTNGINGHGVNGETAKPSLRLSFAEYKRISNLLVLHLRRAEEDEDEAALKKSEVVNWYLKEIESEIDSEMELISKKTMIEKVIYRLVHYDYILIELKQSGLKGSAESSETESQEEDVTLVVNPNYTFED

Target ORF information:

RefSeq Version	NM_001082849.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio minichromosome maintenance complex component 6 (mcm6), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001082849.1

ORF Insert Sequence:

ATGGATCTTA CGGAGACTGG AGTGAATCAG AACGCTGGCC AGGTGATCAA GGACGAGCTG 
GCTGAAAAAT GTCAGAAATT ATTCCAGGCC TTTTTGGAAG AGTTTCAGAA TAGCGATGGT 
GAGGTGAAGT ACCTGCGGGA CGCGGAGGAG CTGATCCGGC CTGAGAGGAA CACACTCGCG 
GTCAGTTTTA CCGACCTTGA GGGATTTAAC CAAGAACTGG CAACCGCCAT CCAGGAGGAA 
TTCTATAGGC TATACCCCTA CTTGTGCCGT GCTGTTCGTA ATTTTGCTCG GGAACATGGA 
GAAGTTCCCA CCTCCAAGGA ATTCTACGTG GCCTTCCAGG ATCTACCAAC CAGACACAAG 
ATTCGTGAGT TGACCACAGT GAAGGTCGGT TCACTGGTGC GTATCAGCGG TCAGGTGGTC 
CGTACTCACC CGGTTCACCC TGAGCTGGTG AGCGGGACCT TTCTGTGTTT GGACTGTCAG 
GGAGTGATAA AAGACGTGGA GCAGCAGTTT AAATACACTC AGCCCAGCAT TTGCCGCAAT 
CCTGTGTGCA ACAACCGCAG ACGCTTTCTC CTAGATACCA ACAAGTCCAA ATTCATTGAC 
TTCCAGAAGT TACGCATCCA AGAGACGCAG GCTGAGCTGC CCCGTGGCTC CATTCCTCGC 
AGTATGGAGG TGATCCTGCG GGCCGAGGCT GTGGAGTCAG CACAGGCCGG GGATAAATGT 
GACTTCATTG GCTCCCTCAT CGTTGTGCCT GATATATCCC AGTTGGCCAC ACCAGGTGTG 
CGCGCTGAGA CCAGCTCTCG TACGGCAGGA GTCCAGGGCT ATGAGAACGA AGGTTTGCGT 
GGGCTTAAAG CGCTGGGCGT CAGGGAACTG TCCTACAAAC TGGCTTTCTT GGCGTGCCAC 
GTTGCCCCCA CCAATCCCAG ATTTGGAGGC AAGGAGATTC GAGATGAGGA GCAAACGGCA 
GAGAGTATCA AGAAACAGAT GTCAGTGCAG GAGTGGGAGA AGGTGTTTGA GATGAGTCAA 
GATAAAAACC TCTACCATAA CCTCTGCACC AGCCTGTTCC CTACTATTCA CGGTAATGAT 
GAGGTAAAGC GAGGAATTCT GTTGATGCTG TTCGGAGGTG TTCCCAAAAC CACCATGGAG 
GGAACGTCTT TGAGAGGAGA CATCAACGTT TGTGTTGTAG GAGATCCAAG CACAGCTAAA 
AGCCAGTTCC TCAAGCATGT GGAGGAGTTC TGTCCTCGGG CCGTCTACAC CAGCGGCAAA 
GCCTCCAGCG CTGCCGGTCT CACAGCTGCT GTGGTGAGAG ATGAGGAGTC ACACGAGTTC 
GTCATTGAAG CCGGAGCTCT CATGCTGGCT GACAACGGTG TTTGCTGCAT TGACGAGTTT 
GATAAGATGG AAACGAGAGA CCAGGTGGCC ATCCATGAAG CCATGGAGCA GCAGACCATC 
TCCATCACCA AAGCAGGAGT CAAGGCCACT CTGAATGCTC GCACATCCAT CTTGGCTGCA 
GCAAATCCAG TGAGCGGCCG TTACGACCGA TCCAAATCTC TCAAACAGAA CGTCAACCTG 
ACGGCACCCA TCATGTCTCG ATTTGACCTA TTCTTCATCC TTGTGGATGA CTGCAATGAG 
GTCACTGATT ATGCTATAGC CAGACGTATA GTGGATCTCC ACTCCAGGAT TGAGAATTCT 
GTCGACCGCG TCTACAGTCT CGATGAGATT CGCAGATACC TGCTGTTTGC TCGCCAGTTC 
AAGCCAAAGA TCTCAAAAGA GTCTGAAGAG TTTATCGTGG AGCAGTACAA GCGACTGAGA 
CAGCGGGACG GGTCCGGAGT CACAAAGTCA GCCTGGAGGA TCACAGTCCG CCAGCTGGAG 
AGCTTGATCC GGCTCTCTGA GAGCATGGCC AGGATGCACT GCTGCGATGA GGTTCAACCA 
AAGCATGTCA AGGAGGCTTT CAGGCTGCTG AACAAGTCTA TTATTCGGGT TGAGACGCCT 
GATGTTAATC TGGATCAAGA GGAAGAGGAG GCTATGGAGG AGGAAGAGGA CAATCAGATG 
AATGGGCACG ATGTACCTAA CGGTGTAAAT GGAGCTGTTA ATGGAGAAGT GAATGGCCAC 
GAGCATACAA ATGGTATCAA CGGTCACGGG GTCAATGGAG AAACTGCAAA ACCCTCACTG 
AGACTGTCGT TTGCTGAGTA TAAACGCATT TCAAACCTGC TGGTGCTGCA TCTGCGTCGT 
GCTGAGGAAG ATGAGGACGA GGCGGCGCTG AAGAAGAGTG AAGTTGTGAA CTGGTACCTG 
AAGGAGATCG AATCAGAGAT TGATTCTGAA ATGGAGCTCA TCAGTAAGAA GACCATGATA 
GAGAAAGTGA TCTACAGACT GGTTCACTAT GATTATATTC TGATTGAGTT AAAACAGTCC 
GGGCTGAAAG GGTCTGCTGA GTCCAGTGAA ACAGAATCGC AGGAGGAAGA CGTTACGCTG 
GTGGTCAATC CCAACTACAC TTTCGAAGAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cohesin facilitates zygotic genome activation in zebrafish.
Development (Cambridge, England)145(1)(2018 Jan)
Meier M,Grant J,Dowdle A,Thomas A,Gerton J,Collas P,O'Sullivan JM,Horsfield JA

DHX33 Transcriptionally Controls Genes Involved in the Cell Cycle.
Molecular and cellular biology36(23)2903-2917(2016 Dec)
Yuan B,Wang X,Fan C,You J,Liu Y,Weber JD,Zhong H,Zhang Y

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Telomerase Is Essential for Zebrafish Heart Regeneration.
Cell reports12(10)1691-703(2015 Sep)
Bednarek D,Gonz?lez-Rosa JM,Guzm?n-Mart?nez G,Guti?rrez-Guti?rrez ?,Aguado T,S?nchez-Ferrer C,Marques IJ,Galardi-Castilla M,de Diego I,G?mez MJ,Cort?s A,Zapata A,Jim?nez-Borreguero LJ,Mercader N,Flores I

Evolutionary diversification of MCM3 genes in Xenopus laevis and Danio rerio.
Cell cycle (Georgetown, Tex.)13(20)3271-81(2014)
Shinya M,Machiki D,Henrich T,Kubota Y,Takisawa H,Mimura S

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mcm6 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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