edrf1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	edrf1
Entrez Gene ID	566404
Full Name	erythroid differentiation regulatory factor 1
Synonyms	gb:gw712215,si:rp71-40n14.4
General protein information	Preferred Names erythroid differentiation regulatory factor 1 Names erythroid differentiation-related factor 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_689672.8	XP_694764.4	erythroid differentiation-related factor 1
XM_021473083.1	XP_021328758.1	erythroid differentiation-related factor 1 isoform X2
XM_689672.9	XP_694764.4	erythroid differentiation-related factor 1 isoform X2
XM_021467293.1	XP_021322968.1	erythroid differentiation-related factor 1 isoform X1
XM_021473082.1	XP_021328757.1	erythroid differentiation-related factor 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	EDRF1	NP_001189367.1
Drosophila melanogaster (fruit fly)	CG6511	NP_648252.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001324	XP_321822.5
	LOC695685	XP_002805895.1
Canis lupus familiaris (dog)	C28H10orf137	XP_003640049.1
Danio rerio (zebrafish)	LOC566404	XP_694764.4
Xenopus tropicalis (tropical clawed frog)	LOC100497935	XP_004912778.1
Pan troglodytes (chimpanzee)	C10H10orf137	XP_001137992.1
Mus musculus (house mouse)	Edrf1	NP_835216.3
Rattus norvegicus (Norway rat)	RGD1306820	NP_001101027.1
Gallus gallus (chicken)	C6H10ORF137	XP_428227.4
Bos taurus (cattle)	C26H10orf137	XP_005225922.1

Gene Ontology

The following edrf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the edrf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa145847	XM_021467293.1 Latest version!	Danio rerio erythroid differentiation regulatory factor 1 (edrf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa145847	XM_021473082.1 Latest version!	Danio rerio erythroid differentiation regulatory factor 1 (edrf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa22247	XM_021473083.1 Latest version!	Danio rerio erythroid differentiation regulatory factor 1 (edrf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa22247	XM_689672.9 Latest version!	Danio rerio erythroid differentiation regulatory factor 1 (edrf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa22247	XM_689672.8	Danio rerio erythroid differentiation regulatory factor 1 (edrf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

edrf1 ( XM_021467293.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021322968.1 Danio rerio erythroid differentiation regulatory factor 1 (edrf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa145847
Clone ID Related Accession (Same CDS sequence)	XM_021467293.1 , XM_021473082.1
Accession Version	XM_021467293.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	erythroid differentiation-related factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGC TGAATATGAG CTCTCAGGAT AAGACAGACA CAGATCTTAG ACAGGAGGAG 
GAACACAGCA GCTCTGAGGA CAACAAACAG GAGGGGGTTA TTTGTCTGGG CAACAATGAA 
GTGAAGAGTC GTGCAGTAGT GAAGTATTCG GCTGCTCCTC CTCCCTCCTC CTATGCATTA 
CTACAAGAGA AGACCGACCT CAAGCTGCCA CCAGCAAACT GGCTGCGCGA AAATGCTCAG 
CTGGGACCCG CAGGGACCAC CATACTCGGG TCCAGCCGGA AAAGCAAACC CTTCTCCAGT 
TTTGGGATGG CGTTTGACTT CATTGACTGT ATAGGTGATG ATGTCGATGT TGTGTCTGAT 
TCAGAGAACA TCAAGAAGCT TCTGAAGATC CCGTACAGTA AATCTCATGT CAGCATGGCT 
GTTCATAGAG TTGGCAGCAC TTTACTGTTG GACGAACTGG ACATTCAGGA GCTCTTCATG 
AGGTCATCAC AGACTAATGA CTGGACGTGG CTGAAGGAGT TTTACCAGCG TCTTATAGAC 
CAAAAGTGGC AGAGAAAGAA GAAAAGTAAA GAGCACTGGA ACGAGAAGGC CATCCTCTCC 
AAATTCCTCT ATTACAGTAT AAACAGTGAT GGGACTGCAG TGTCTGTGCC GTCAGATACA 
GAGCAAACAG GAGACGAGGG GTGTGGGGCA GGAACTGATG GACCCTCTTG GCCTGCGACT 
TTCAGCAGCT CCACCACTGA TTCAGAAGAG TCTGCACTGC CCAAAGAGGA GCAGGTGGAC 
ACATATGCTT TAGGTCACGT GTCGTCGGTT CCCAAAGAAC AGAATCTACC CACGCTGTTT 
AATGAAGGAG AAAACAGTCA GGGTTTGAGA AATGACTTTG TCCGAAATAT TTTATGGACA 
TTTGAGGACA TCCACATGCT TGTGGGATCA AATATGCCAA TTTTCGGAGG TGGACGATAC 
CCAGCAGTCA GTTTACGTCT CAGGGACAAC AACAAACCAA TCAACGTTCT CACAGGAATT 
GACTACTGGC TGGATAATTT GATGTGTAAC GTACCAGAGC TGGTCATGTG CTTTCACGTC 
AATGGCATTG TTCAGAAATA TGAAATGATA AAAACAGAGG ACATCCCCAA TTTAGAGAAC 
TCCACATTCT CCACGCGGGT TGTGAAAGAC ATTGCTCAAA ATATCCTGTC GTTCCTGAAA 
TCAAACTGTA CCAAAGAAGG ACACACGTAC TGGCTCTTCA AGGCCAGCGG GAGTGACATT 
GTGAAGCTGT ACGACCTCAC TACACTGTGT GAAGAAGCAG CAGAGGAGAA ATGCCAGAAT 
CCCTTCACAC TTCCAGTAGC CGTGCTTCTC TACAAAGTGG CCAGCAACAT GATGCTAAAG 
AAGAGCCAAA ACCGGAAGAT CTATGGGACA ATCAGAACTC TACTGCTCAA CTGTATTAAA 
CTGTTGGATG AGGACAGACA TCCACAGATC ATCGCTTCGG CTCACTACAT GCTGGCAGAA 
CTGTTTCAGC TGGATGATGT GAGTGAAGAC GAAGGCAATG GGGAGTCGCT GAGAGGAGGA 
GGATCTGAAG ACAGCTACAG TGATGAAGAT GAGGAGGATG AGGAGGAAGA TGAAGACGAG 
AGATGCTCCT TCAGCACTGC CTCTGAACCG CAGCACGACT CCAAAGCTGT GGCCGTCATC 
AAGTCTGTCG GAGAGCTTTC GGTTCCAGAG AAATACAAGA GCACGCATCA GATCAGACCA 
AACTTTGCAT ATCCTGTTTC ACAAGACAAG GAGGAGAAGT GCCGACTGGT TCTCAGCTAC 
GTTTTAAAAG GTCTGAAAGC AGTGGACAGC AGCATAAAGA AAGAGAGCGA CCTTCCTGCA 
GCCGATCCCA GCACACCCAT CCCACTAAAA TATGAGGAAA GAAGTCCAAA TGGAGCTAGA 
GAGGTGGAGC AGGAGATTGC TTTACTGCTG GATAGAGTTG GCCCATGTAA CGAAGAGTTG 
AAGGTGCCGA CACGCTCCGG GATGTTACCA GGCACATGGC AGCATCGCAT GAAACTCCAG 
CTGTTCCTGA AAGCATCGAA AGCGTATTAC GTTTTGTCAG ATGCTGCGAC CAACCTACTG 
AAGTACGGCC GGGCACTGCG CTACATCAAA CTGGCTCTGC AGTGCCACGA TGCATACTGC 
TCCATTAGTG GCGGTCTCCA CGCACAGGTG CTTCTTTTCC ACTGCCAGTG TCTGTCTCTG 
TGTGGAGATG TTCAGCTCAT GTTGGCCCAG AATGCCTCAA ACAGAGCTGC GTATCTGGAG 
GAGTACAGCT ATCAGACCAA AGATGACCAG GAGATCCTCC ACTGCCTGCA CCGCGAGAGC 
TCCTGCCAAG CCTTCAGCAT GGCCACTGAT CTGGCCCTGG ATCCTGAGTA CCAGCTTATC 
GTCAGCTGCA AGTGTTATGA AGCCGCTCAT GATCTTTTGA CGTCAGGAGT CCTGAAAGAC 
CAGGCACAGG AGCAGCTGGG TCAGGTGCTG AAACGTCTCG GGAACATTCG AAACGAGATG 
GGCGTTTTCT ACATGAACCA GGCTGCAGCA ATGCAGGCAG AGAAAGAAGG AGGTCGTAAA 
AAAGACTGTA CAAATGCTAA GAAAACCAGG AGTAAAAGTA TAATTTTCTT TTCTGGTCCG 
GACCAGAAGA ACCCAGAAAA CCAAGCTACA ATGCGCAGGA GTGTGTGTGT GGCCGAGCAG 
GAGCTGTGGA AGAAGAGCTT CTCGTGTTTT GAGAAGGGCA TGCAGTATTT TAGTGCTATT 
TCAGACTCGG TGAACACGGC CCTGCTGCTG TGTAACATCG GCCGTCTCAT GCGCATCTGT 
GCTCAGGCTC ACTGCGTCAT TACGTCAGAG CAGAGCAGAG GAGAGTTTTC ACCTGAAGAG 
ACTCTCTATT ACAACAAGGC CATAGATTAT TATCAGAGAG CGCTGAAGGC TCTGGGCTGC 
AGAGAGAGTC ACTCCGCCGT CTGGGACTCG GTCAACTGGG AACTGTCCAC AACCTATTTC 
ACACTGGCCA CACTCCTGCA GGATTACGCC CCACTGTCCC GCAAAGCACA GGAGCAGATC 
GAGAGGGAAG TGACCGAGAC CATGATGAAG TCTCTGAGAT ACTGTGACCT GCAGACTGAA 
TCCGCTCGCC AGCCGCTCTA CCAGTACAGA GCAGCCACAA TCCACCACAG ACTGGCTTCA 
ATGTACCACA GCTGCTACCG CAATCAGGTT GGAGATGAGA GTGATCGTAA GCAGCACAAA 
GCTCTGGCAG AGCAGCACTA CAGTAAAGCC GTGCGCCTCT TTGTGAATCT GAGTGATGCT 
CCGTGTGAAC TGCTCCGCAC ACTTCTGGAG AGAGTGGCCT TCGCTGAGTT CACCATGGCA 
GGTCAGAACA GCAGTGCTGT GAAACTGAAG ACTCTGACAG GAGCTTTGGA GATCATGTGT 
GAAACACGGC ATGCGTTCAG ACTCATTCAT AAAGAGCTGC TGGAGCAACA AGAAAATATA 
ACGAAGGCTG AAGAGAAACC TGAAGAAGTC CCTGAGGGTT CAGAGAGTTC CTCTTCCTCT 
GAGCTGAACA CAGAAGAGGT GCTGAAGCTA ATTGGCATCT TCGAGCCCAG TTTCTCCTTC 
CTGTTACTGC AAGTGATTAA ACTCAGCTCT GCAAGCAAAC GAAAACTCAG TGGTAAAGAA 
GAGGAGCAGG TGAAGATCTA CAAGAGCGTC TATTCTCGTC TCTTACGGGC TGAAAAGTCT 
GATCCACTCA TTCAGCGGGT TTCGCTTTTC CTGGAGCTCC TGGATCAGCT GAATCCACCA 
TCTGGAAGTG AAGAACCAAG ATCATCATCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021322968.1
CDS	371..4183
Translation	MSVLNMSSQDKTDTDLRQEEEHSSSEDNKQEGVICLGNNEVKSRAVVKYSAAPPPSSYALLQEKTDLKLPPANWLRENAQLGPAGTTILGSSRKSKPFSSFGMAFDFIDCIGDDVDVVSDSENIKKLLKIPYSKSHVSMAVHRVGSTLLLDELDIQELFMRSSQTNDWTWLKEFYQRLIDQKWQRKKKSKEHWNEKAILSKFLYYSINSDGTAVSVPSDTEQTGDEGCGAGTDGPSWPATFSSSTTDSEESALPKEEQVDTYALGHVSSVPKEQNLPTLFNEGENSQGLRNDFVRNILWTFEDIHMLVGSNMPIFGGGRYPAVSLRLRDNNKPINVLTGIDYWLDNLMCNVPELVMCFHVNGIVQKYEMIKTEDIPNLENSTFSTRVVKDIAQNILSFLKSNCTKEGHTYWLFKASGSDIVKLYDLTTLCEEAAEEKCQNPFTLPVAVLLYKVASNMMLKKSQNRKIYGTIRTLLLNCIKLLDEDRHPQIIASAHYMLAELFQLDDVSEDEGNGESLRGGGSEDSYSDEDEEDEEEDEDERCSFSTASEPQHDSKAVAVIKSVGELSVPEKYKSTHQIRPNFAYPVSQDKEEKCRLVLSYVLKGLKAVDSSIKKESDLPAADPSTPIPLKYEERSPNGAREVEQEIALLLDRVGPCNEELKVPTRSGMLPGTWQHRMKLQLFLKASKAYYVLSDAATNLLKYGRALRYIKLALQCHDAYCSISGGLHAQVLLFHCQCLSLCGDVQLMLAQNASNRAAYLEEYSYQTKDDQEILHCLHRESSCQAFSMATDLALDPEYQLIVSCKCYEAAHDLLTSGVLKDQAQEQLGQVLKRLGNIRNEMGVFYMNQAAAMQAEKEGGRKKDCTNAKKTRSKSIIFFSGPDQKNPENQATMRRSVCVAEQELWKKSFSCFEKGMQYFSAISDSVNTALLLCNIGRLMRICAQAHCVITSEQSRGEFSPEETLYYNKAIDYYQRALKALGCRESHSAVWDSVNWELSTTYFTLATLLQDYAPLSRKAQEQIEREVTETMMKSLRYCDLQTESARQPLYQYRAATIHHRLASMYHSCYRNQVGDESDRKQHKALAEQHYSKAVRLFVNLSDAPCELLRTLLERVAFAEFTMAGQNSSAVKLKTLTGALEIMCETRHAFRLIHKELLEQQENITKAEEKPEEVPEGSESSSSSELNTEEVLKLIGIFEPSFSFLLLQVIKLSSASKRKLSGKEEEQVKIYKSVYSRLLRAEKSDPLIQRVSLFLELLDQLNPPSGSEEPRSSS

Target ORF information:

RefSeq Version	XM_021467293.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio erythroid differentiation regulatory factor 1 (edrf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021467293.1

ORF Insert Sequence:

ATGAGTGTGC TGAATATGAG CTCTCAGGAT AAGACAGACA CAGATCTTAG ACAGGAGGAG 
GAACACAGCA GCTCTGAGGA CAACAAACAG GAGGGGGTTA TTTGTCTGGG CAACAATGAA 
GTGAAGAGTC GTGCAGTAGT GAAGTATTCG GCTGCTCCTC CTCCCTCCTC CTATGCATTA 
CTACAAGAGA AGACCGACCT CAAGCTGCCA CCAGCAAACT GGCTGCGCGA AAATGCTCAG 
CTGGGACCCG CAGGGACCAC CATACTCGGG TCCAGCCGGA AAAGCAAACC CTTCTCCAGT 
TTTGGGATGG CGTTTGACTT CATTGACTGT ATAGGTGATG ATGTCGATGT TGTGTCTGAT 
TCAGAGAACA TCAAGAAGCT TCTGAAGATC CCGTACAGTA AATCTCATGT CAGCATGGCT 
GTTCATAGAG TTGGCAGCAC TTTACTGTTG GACGAACTGG ACATTCAGGA GCTCTTCATG 
AGGTCATCAC AGACTAATGA CTGGACGTGG CTGAAGGAGT TTTACCAGCG TCTTATAGAC 
CAAAAGTGGC AGAGAAAGAA GAAAAGTAAA GAGCACTGGA ACGAGAAGGC CATCCTCTCC 
AAATTCCTCT ATTACAGTAT AAACAGTGAT GGGACTGCAG TGTCTGTGCC GTCAGATACA 
GAGCAAACAG GAGACGAGGG GTGTGGGGCA GGAACTGATG GACCCTCTTG GCCTGCGACT 
TTCAGCAGCT CCACCACTGA TTCAGAAGAG TCTGCACTGC CCAAAGAGGA GCAGGTGGAC 
ACATATGCTT TAGGTCACGT GTCGTCGGTT CCCAAAGAAC AGAATCTACC CACGCTGTTT 
AATGAAGGAG AAAACAGTCA GGGTTTGAGA AATGACTTTG TCCGAAATAT TTTATGGACA 
TTTGAGGACA TCCACATGCT TGTGGGATCA AATATGCCAA TTTTCGGAGG TGGACGATAC 
CCAGCAGTCA GTTTACGTCT CAGGGACAAC AACAAACCAA TCAACGTTCT CACAGGAATT 
GACTACTGGC TGGATAATTT GATGTGTAAC GTACCAGAGC TGGTCATGTG CTTTCACGTC 
AATGGCATTG TTCAGAAATA TGAAATGATA AAAACAGAGG ACATCCCCAA TTTAGAGAAC 
TCCACATTCT CCACGCGGGT TGTGAAAGAC ATTGCTCAAA ATATCCTGTC GTTCCTGAAA 
TCAAACTGTA CCAAAGAAGG ACACACGTAC TGGCTCTTCA AGGCCAGCGG GAGTGACATT 
GTGAAGCTGT ACGACCTCAC TACACTGTGT GAAGAAGCAG CAGAGGAGAA ATGCCAGAAT 
CCCTTCACAC TTCCAGTAGC CGTGCTTCTC TACAAAGTGG CCAGCAACAT GATGCTAAAG 
AAGAGCCAAA ACCGGAAGAT CTATGGGACA ATCAGAACTC TACTGCTCAA CTGTATTAAA 
CTGTTGGATG AGGACAGACA TCCACAGATC ATCGCTTCGG CTCACTACAT GCTGGCAGAA 
CTGTTTCAGC TGGATGATGT GAGTGAAGAC GAAGGCAATG GGGAGTCGCT GAGAGGAGGA 
GGATCTGAAG ACAGCTACAG TGATGAAGAT GAGGAGGATG AGGAGGAAGA TGAAGACGAG 
AGATGCTCCT TCAGCACTGC CTCTGAACCG CAGCACGACT CCAAAGCTGT GGCCGTCATC 
AAGTCTGTCG GAGAGCTTTC GGTTCCAGAG AAATACAAGA GCACGCATCA GATCAGACCA 
AACTTTGCAT ATCCTGTTTC ACAAGACAAG GAGGAGAAGT GCCGACTGGT TCTCAGCTAC 
GTTTTAAAAG GTCTGAAAGC AGTGGACAGC AGCATAAAGA AAGAGAGCGA CCTTCCTGCA 
GCCGATCCCA GCACACCCAT CCCACTAAAA TATGAGGAAA GAAGTCCAAA TGGAGCTAGA 
GAGGTGGAGC AGGAGATTGC TTTACTGCTG GATAGAGTTG GCCCATGTAA CGAAGAGTTG 
AAGGTGCCGA CACGCTCCGG GATGTTACCA GGCACATGGC AGCATCGCAT GAAACTCCAG 
CTGTTCCTGA AAGCATCGAA AGCGTATTAC GTTTTGTCAG ATGCTGCGAC CAACCTACTG 
AAGTACGGCC GGGCACTGCG CTACATCAAA CTGGCTCTGC AGTGCCACGA TGCATACTGC 
TCCATTAGTG GCGGTCTCCA CGCACAGGTG CTTCTTTTCC ACTGCCAGTG TCTGTCTCTG 
TGTGGAGATG TTCAGCTCAT GTTGGCCCAG AATGCCTCAA ACAGAGCTGC GTATCTGGAG 
GAGTACAGCT ATCAGACCAA AGATGACCAG GAGATCCTCC ACTGCCTGCA CCGCGAGAGC 
TCCTGCCAAG CCTTCAGCAT GGCCACTGAT CTGGCCCTGG ATCCTGAGTA CCAGCTTATC 
GTCAGCTGCA AGTGTTATGA AGCCGCTCAT GATCTTTTGA CGTCAGGAGT CCTGAAAGAC 
CAGGCACAGG AGCAGCTGGG TCAGGTGCTG AAACGTCTCG GGAACATTCG AAACGAGATG 
GGCGTTTTCT ACATGAACCA GGCTGCAGCA ATGCAGGCAG AGAAAGAAGG AGGTCGTAAA 
AAAGACTGTA CAAATGCTAA GAAAACCAGG AGTAAAAGTA TAATTTTCTT TTCTGGTCCG 
GACCAGAAGA ACCCAGAAAA CCAAGCTACA ATGCGCAGGA GTGTGTGTGT GGCCGAGCAG 
GAGCTGTGGA AGAAGAGCTT CTCGTGTTTT GAGAAGGGCA TGCAGTATTT TAGTGCTATT 
TCAGACTCGG TGAACACGGC CCTGCTGCTG TGTAACATCG GCCGTCTCAT GCGCATCTGT 
GCTCAGGCTC ACTGCGTCAT TACGTCAGAG CAGAGCAGAG GAGAGTTTTC ACCTGAAGAG 
ACTCTCTATT ACAACAAGGC CATAGATTAT TATCAGAGAG CGCTGAAGGC TCTGGGCTGC 
AGAGAGAGTC ACTCCGCCGT CTGGGACTCG GTCAACTGGG AACTGTCCAC AACCTATTTC 
ACACTGGCCA CACTCCTGCA GGATTACGCC CCACTGTCCC GCAAAGCACA GGAGCAGATC 
GAGAGGGAAG TGACCGAGAC CATGATGAAG TCTCTGAGAT ACTGTGACCT GCAGACTGAA 
TCCGCTCGCC AGCCGCTCTA CCAGTACAGA GCAGCCACAA TCCACCACAG ACTGGCTTCA 
ATGTACCACA GCTGCTACCG CAATCAGGTT GGAGATGAGA GTGATCGTAA GCAGCACAAA 
GCTCTGGCAG AGCAGCACTA CAGTAAAGCC GTGCGCCTCT TTGTGAATCT GAGTGATGCT 
CCGTGTGAAC TGCTCCGCAC ACTTCTGGAG AGAGTGGCCT TCGCTGAGTT CACCATGGCA 
GGTCAGAACA GCAGTGCTGT GAAACTGAAG ACTCTGACAG GAGCTTTGGA GATCATGTGT 
GAAACACGGC ATGCGTTCAG ACTCATTCAT AAAGAGCTGC TGGAGCAACA AGAAAATATA 
ACGAAGGCTG AAGAGAAACC TGAAGAAGTC CCTGAGGGTT CAGAGAGTTC CTCTTCCTCT 
GAGCTGAACA CAGAAGAGGT GCTGAAGCTA ATTGGCATCT TCGAGCCCAG TTTCTCCTTC 
CTGTTACTGC AAGTGATTAA ACTCAGCTCT GCAAGCAAAC GAAAACTCAG TGGTAAAGAA 
GAGGAGCAGG TGAAGATCTA CAAGAGCGTC TATTCTCGTC TCTTACGGGC TGAAAAGTCT 
GATCCACTCA TTCAGCGGGT TTCGCTTTTC CTGGAGCTCC TGGATCAGCT GAATCCACCA 
TCTGGAAGTG AAGAACCAAG ATCATCATCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

edrf1 ( XM_021473082.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328757.1 Danio rerio erythroid differentiation regulatory factor 1 (edrf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa145847
Clone ID Related Accession (Same CDS sequence)	XM_021467293.1 , XM_021473082.1
Accession Version	XM_021473082.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3813bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	erythroid differentiation-related factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394977.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGC TGAATATGAG CTCTCAGGAT AAGACAGACA CAGATCTTAG ACAGGAGGAG 
GAACACAGCA GCTCTGAGGA CAACAAACAG GAGGGGGTTA TTTGTCTGGG CAACAATGAA 
GTGAAGAGTC GTGCAGTAGT GAAGTATTCG GCTGCTCCTC CTCCCTCCTC CTATGCATTA 
CTACAAGAGA AGACCGACCT CAAGCTGCCA CCAGCAAACT GGCTGCGCGA AAATGCTCAG 
CTGGGACCCG CAGGGACCAC CATACTCGGG TCCAGCCGGA AAAGCAAACC CTTCTCCAGT 
TTTGGGATGG CGTTTGACTT CATTGACTGT ATAGGTGATG ATGTCGATGT TGTGTCTGAT 
TCAGAGAACA TCAAGAAGCT TCTGAAGATC CCGTACAGTA AATCTCATGT CAGCATGGCT 
GTTCATAGAG TTGGCAGCAC TTTACTGTTG GACGAACTGG ACATTCAGGA GCTCTTCATG 
AGGTCATCAC AGACTAATGA CTGGACGTGG CTGAAGGAGT TTTACCAGCG TCTTATAGAC 
CAAAAGTGGC AGAGAAAGAA GAAAAGTAAA GAGCACTGGA ACGAGAAGGC CATCCTCTCC 
AAATTCCTCT ATTACAGTAT AAACAGTGAT GGGACTGCAG TGTCTGTGCC GTCAGATACA 
GAGCAAACAG GAGACGAGGG GTGTGGGGCA GGAACTGATG GACCCTCTTG GCCTGCGACT 
TTCAGCAGCT CCACCACTGA TTCAGAAGAG TCTGCACTGC CCAAAGAGGA GCAGGTGGAC 
ACATATGCTT TAGGTCACGT GTCGTCGGTT CCCAAAGAAC AGAATCTACC CACGCTGTTT 
AATGAAGGAG AAAACAGTCA GGGTTTGAGA AATGACTTTG TCCGAAATAT TTTATGGACA 
TTTGAGGACA TCCACATGCT TGTGGGATCA AATATGCCAA TTTTCGGAGG TGGACGATAC 
CCAGCAGTCA GTTTACGTCT CAGGGACAAC AACAAACCAA TCAACGTTCT CACAGGAATT 
GACTACTGGC TGGATAATTT GATGTGTAAC GTACCAGAGC TGGTCATGTG CTTTCACGTC 
AATGGCATTG TTCAGAAATA TGAAATGATA AAAACAGAGG ACATCCCCAA TTTAGAGAAC 
TCCACATTCT CCACGCGGGT TGTGAAAGAC ATTGCTCAAA ATATCCTGTC GTTCCTGAAA 
TCAAACTGTA CCAAAGAAGG ACACACGTAC TGGCTCTTCA AGGCCAGCGG GAGTGACATT 
GTGAAGCTGT ACGACCTCAC TACACTGTGT GAAGAAGCAG CAGAGGAGAA ATGCCAGAAT 
CCCTTCACAC TTCCAGTAGC CGTGCTTCTC TACAAAGTGG CCAGCAACAT GATGCTAAAG 
AAGAGCCAAA ACCGGAAGAT CTATGGGACA ATCAGAACTC TACTGCTCAA CTGTATTAAA 
CTGTTGGATG AGGACAGACA TCCACAGATC ATCGCTTCGG CTCACTACAT GCTGGCAGAA 
CTGTTTCAGC TGGATGATGT GAGTGAAGAC GAAGGCAATG GGGAGTCGCT GAGAGGAGGA 
GGATCTGAAG ACAGCTACAG TGATGAAGAT GAGGAGGATG AGGAGGAAGA TGAAGACGAG 
AGATGCTCCT TCAGCACTGC CTCTGAACCG CAGCACGACT CCAAAGCTGT GGCCGTCATC 
AAGTCTGTCG GAGAGCTTTC GGTTCCAGAG AAATACAAGA GCACGCATCA GATCAGACCA 
AACTTTGCAT ATCCTGTTTC ACAAGACAAG GAGGAGAAGT GCCGACTGGT TCTCAGCTAC 
GTTTTAAAAG GTCTGAAAGC AGTGGACAGC AGCATAAAGA AAGAGAGCGA CCTTCCTGCA 
GCCGATCCCA GCACACCCAT CCCACTAAAA TATGAGGAAA GAAGTCCAAA TGGAGCTAGA 
GAGGTGGAGC AGGAGATTGC TTTACTGCTG GATAGAGTTG GCCCATGTAA CGAAGAGTTG 
AAGGTGCCGA CACGCTCCGG GATGTTACCA GGCACATGGC AGCATCGCAT GAAACTCCAG 
CTGTTCCTGA AAGCATCGAA AGCGTATTAC GTTTTGTCAG ATGCTGCGAC CAACCTACTG 
AAGTACGGCC GGGCACTGCG CTACATCAAA CTGGCTCTGC AGTGCCACGA TGCATACTGC 
TCCATTAGTG GCGGTCTCCA CGCACAGGTG CTTCTTTTCC ACTGCCAGTG TCTGTCTCTG 
TGTGGAGATG TTCAGCTCAT GTTGGCCCAG AATGCCTCAA ACAGAGCTGC GTATCTGGAG 
GAGTACAGCT ATCAGACCAA AGATGACCAG GAGATCCTCC ACTGCCTGCA CCGCGAGAGC 
TCCTGCCAAG CCTTCAGCAT GGCCACTGAT CTGGCCCTGG ATCCTGAGTA CCAGCTTATC 
GTCAGCTGCA AGTGTTATGA AGCCGCTCAT GATCTTTTGA CGTCAGGAGT CCTGAAAGAC 
CAGGCACAGG AGCAGCTGGG TCAGGTGCTG AAACGTCTCG GGAACATTCG AAACGAGATG 
GGCGTTTTCT ACATGAACCA GGCTGCAGCA ATGCAGGCAG AGAAAGAAGG AGGTCGTAAA 
AAAGACTGTA CAAATGCTAA GAAAACCAGG AGTAAAAGTA TAATTTTCTT TTCTGGTCCG 
GACCAGAAGA ACCCAGAAAA CCAAGCTACA ATGCGCAGGA GTGTGTGTGT GGCCGAGCAG 
GAGCTGTGGA AGAAGAGCTT CTCGTGTTTT GAGAAGGGCA TGCAGTATTT TAGTGCTATT 
TCAGACTCGG TGAACACGGC CCTGCTGCTG TGTAACATCG GCCGTCTCAT GCGCATCTGT 
GCTCAGGCTC ACTGCGTCAT TACGTCAGAG CAGAGCAGAG GAGAGTTTTC ACCTGAAGAG 
ACTCTCTATT ACAACAAGGC CATAGATTAT TATCAGAGAG CGCTGAAGGC TCTGGGCTGC 
AGAGAGAGTC ACTCCGCCGT CTGGGACTCG GTCAACTGGG AACTGTCCAC AACCTATTTC 
ACACTGGCCA CACTCCTGCA GGATTACGCC CCACTGTCCC GCAAAGCACA GGAGCAGATC 
GAGAGGGAAG TGACCGAGAC CATGATGAAG TCTCTGAGAT ACTGTGACCT GCAGACTGAA 
TCCGCTCGCC AGCCGCTCTA CCAGTACAGA GCAGCCACAA TCCACCACAG ACTGGCTTCA 
ATGTACCACA GCTGCTACCG CAATCAGGTT GGAGATGAGA GTGATCGTAA GCAGCACAAA 
GCTCTGGCAG AGCAGCACTA CAGTAAAGCC GTGCGCCTCT TTGTGAATCT GAGTGATGCT 
CCGTGTGAAC TGCTCCGCAC ACTTCTGGAG AGAGTGGCCT TCGCTGAGTT CACCATGGCA 
GGTCAGAACA GCAGTGCTGT GAAACTGAAG ACTCTGACAG GAGCTTTGGA GATCATGTGT 
GAAACACGGC ATGCGTTCAG ACTCATTCAT AAAGAGCTGC TGGAGCAACA AGAAAATATA 
ACGAAGGCTG AAGAGAAACC TGAAGAAGTC CCTGAGGGTT CAGAGAGTTC CTCTTCCTCT 
GAGCTGAACA CAGAAGAGGT GCTGAAGCTA ATTGGCATCT TCGAGCCCAG TTTCTCCTTC 
CTGTTACTGC AAGTGATTAA ACTCAGCTCT GCAAGCAAAC GAAAACTCAG TGGTAAAGAA 
GAGGAGCAGG TGAAGATCTA CAAGAGCGTC TATTCTCGTC TCTTACGGGC TGAAAAGTCT 
GATCCACTCA TTCAGCGGGT TTCGCTTTTC CTGGAGCTCC TGGATCAGCT GAATCCACCA 
TCTGGAAGTG AAGAACCAAG ATCATCATCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328757.1
CDS	371..4183
Translation	MSVLNMSSQDKTDTDLRQEEEHSSSEDNKQEGVICLGNNEVKSRAVVKYSAAPPPSSYALLQEKTDLKLPPANWLRENAQLGPAGTTILGSSRKSKPFSSFGMAFDFIDCIGDDVDVVSDSENIKKLLKIPYSKSHVSMAVHRVGSTLLLDELDIQELFMRSSQTNDWTWLKEFYQRLIDQKWQRKKKSKEHWNEKAILSKFLYYSINSDGTAVSVPSDTEQTGDEGCGAGTDGPSWPATFSSSTTDSEESALPKEEQVDTYALGHVSSVPKEQNLPTLFNEGENSQGLRNDFVRNILWTFEDIHMLVGSNMPIFGGGRYPAVSLRLRDNNKPINVLTGIDYWLDNLMCNVPELVMCFHVNGIVQKYEMIKTEDIPNLENSTFSTRVVKDIAQNILSFLKSNCTKEGHTYWLFKASGSDIVKLYDLTTLCEEAAEEKCQNPFTLPVAVLLYKVASNMMLKKSQNRKIYGTIRTLLLNCIKLLDEDRHPQIIASAHYMLAELFQLDDVSEDEGNGESLRGGGSEDSYSDEDEEDEEEDEDERCSFSTASEPQHDSKAVAVIKSVGELSVPEKYKSTHQIRPNFAYPVSQDKEEKCRLVLSYVLKGLKAVDSSIKKESDLPAADPSTPIPLKYEERSPNGAREVEQEIALLLDRVGPCNEELKVPTRSGMLPGTWQHRMKLQLFLKASKAYYVLSDAATNLLKYGRALRYIKLALQCHDAYCSISGGLHAQVLLFHCQCLSLCGDVQLMLAQNASNRAAYLEEYSYQTKDDQEILHCLHRESSCQAFSMATDLALDPEYQLIVSCKCYEAAHDLLTSGVLKDQAQEQLGQVLKRLGNIRNEMGVFYMNQAAAMQAEKEGGRKKDCTNAKKTRSKSIIFFSGPDQKNPENQATMRRSVCVAEQELWKKSFSCFEKGMQYFSAISDSVNTALLLCNIGRLMRICAQAHCVITSEQSRGEFSPEETLYYNKAIDYYQRALKALGCRESHSAVWDSVNWELSTTYFTLATLLQDYAPLSRKAQEQIEREVTETMMKSLRYCDLQTESARQPLYQYRAATIHHRLASMYHSCYRNQVGDESDRKQHKALAEQHYSKAVRLFVNLSDAPCELLRTLLERVAFAEFTMAGQNSSAVKLKTLTGALEIMCETRHAFRLIHKELLEQQENITKAEEKPEEVPEGSESSSSSELNTEEVLKLIGIFEPSFSFLLLQVIKLSSASKRKLSGKEEEQVKIYKSVYSRLLRAEKSDPLIQRVSLFLELLDQLNPPSGSEEPRSSS

Target ORF information:

RefSeq Version	XM_021473082.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio erythroid differentiation regulatory factor 1 (edrf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473082.1

ORF Insert Sequence:

ATGAGTGTGC TGAATATGAG CTCTCAGGAT AAGACAGACA CAGATCTTAG ACAGGAGGAG 
GAACACAGCA GCTCTGAGGA CAACAAACAG GAGGGGGTTA TTTGTCTGGG CAACAATGAA 
GTGAAGAGTC GTGCAGTAGT GAAGTATTCG GCTGCTCCTC CTCCCTCCTC CTATGCATTA 
CTACAAGAGA AGACCGACCT CAAGCTGCCA CCAGCAAACT GGCTGCGCGA AAATGCTCAG 
CTGGGACCCG CAGGGACCAC CATACTCGGG TCCAGCCGGA AAAGCAAACC CTTCTCCAGT 
TTTGGGATGG CGTTTGACTT CATTGACTGT ATAGGTGATG ATGTCGATGT TGTGTCTGAT 
TCAGAGAACA TCAAGAAGCT TCTGAAGATC CCGTACAGTA AATCTCATGT CAGCATGGCT 
GTTCATAGAG TTGGCAGCAC TTTACTGTTG GACGAACTGG ACATTCAGGA GCTCTTCATG 
AGGTCATCAC AGACTAATGA CTGGACGTGG CTGAAGGAGT TTTACCAGCG TCTTATAGAC 
CAAAAGTGGC AGAGAAAGAA GAAAAGTAAA GAGCACTGGA ACGAGAAGGC CATCCTCTCC 
AAATTCCTCT ATTACAGTAT AAACAGTGAT GGGACTGCAG TGTCTGTGCC GTCAGATACA 
GAGCAAACAG GAGACGAGGG GTGTGGGGCA GGAACTGATG GACCCTCTTG GCCTGCGACT 
TTCAGCAGCT CCACCACTGA TTCAGAAGAG TCTGCACTGC CCAAAGAGGA GCAGGTGGAC 
ACATATGCTT TAGGTCACGT GTCGTCGGTT CCCAAAGAAC AGAATCTACC CACGCTGTTT 
AATGAAGGAG AAAACAGTCA GGGTTTGAGA AATGACTTTG TCCGAAATAT TTTATGGACA 
TTTGAGGACA TCCACATGCT TGTGGGATCA AATATGCCAA TTTTCGGAGG TGGACGATAC 
CCAGCAGTCA GTTTACGTCT CAGGGACAAC AACAAACCAA TCAACGTTCT CACAGGAATT 
GACTACTGGC TGGATAATTT GATGTGTAAC GTACCAGAGC TGGTCATGTG CTTTCACGTC 
AATGGCATTG TTCAGAAATA TGAAATGATA AAAACAGAGG ACATCCCCAA TTTAGAGAAC 
TCCACATTCT CCACGCGGGT TGTGAAAGAC ATTGCTCAAA ATATCCTGTC GTTCCTGAAA 
TCAAACTGTA CCAAAGAAGG ACACACGTAC TGGCTCTTCA AGGCCAGCGG GAGTGACATT 
GTGAAGCTGT ACGACCTCAC TACACTGTGT GAAGAAGCAG CAGAGGAGAA ATGCCAGAAT 
CCCTTCACAC TTCCAGTAGC CGTGCTTCTC TACAAAGTGG CCAGCAACAT GATGCTAAAG 
AAGAGCCAAA ACCGGAAGAT CTATGGGACA ATCAGAACTC TACTGCTCAA CTGTATTAAA 
CTGTTGGATG AGGACAGACA TCCACAGATC ATCGCTTCGG CTCACTACAT GCTGGCAGAA 
CTGTTTCAGC TGGATGATGT GAGTGAAGAC GAAGGCAATG GGGAGTCGCT GAGAGGAGGA 
GGATCTGAAG ACAGCTACAG TGATGAAGAT GAGGAGGATG AGGAGGAAGA TGAAGACGAG 
AGATGCTCCT TCAGCACTGC CTCTGAACCG CAGCACGACT CCAAAGCTGT GGCCGTCATC 
AAGTCTGTCG GAGAGCTTTC GGTTCCAGAG AAATACAAGA GCACGCATCA GATCAGACCA 
AACTTTGCAT ATCCTGTTTC ACAAGACAAG GAGGAGAAGT GCCGACTGGT TCTCAGCTAC 
GTTTTAAAAG GTCTGAAAGC AGTGGACAGC AGCATAAAGA AAGAGAGCGA CCTTCCTGCA 
GCCGATCCCA GCACACCCAT CCCACTAAAA TATGAGGAAA GAAGTCCAAA TGGAGCTAGA 
GAGGTGGAGC AGGAGATTGC TTTACTGCTG GATAGAGTTG GCCCATGTAA CGAAGAGTTG 
AAGGTGCCGA CACGCTCCGG GATGTTACCA GGCACATGGC AGCATCGCAT GAAACTCCAG 
CTGTTCCTGA AAGCATCGAA AGCGTATTAC GTTTTGTCAG ATGCTGCGAC CAACCTACTG 
AAGTACGGCC GGGCACTGCG CTACATCAAA CTGGCTCTGC AGTGCCACGA TGCATACTGC 
TCCATTAGTG GCGGTCTCCA CGCACAGGTG CTTCTTTTCC ACTGCCAGTG TCTGTCTCTG 
TGTGGAGATG TTCAGCTCAT GTTGGCCCAG AATGCCTCAA ACAGAGCTGC GTATCTGGAG 
GAGTACAGCT ATCAGACCAA AGATGACCAG GAGATCCTCC ACTGCCTGCA CCGCGAGAGC 
TCCTGCCAAG CCTTCAGCAT GGCCACTGAT CTGGCCCTGG ATCCTGAGTA CCAGCTTATC 
GTCAGCTGCA AGTGTTATGA AGCCGCTCAT GATCTTTTGA CGTCAGGAGT CCTGAAAGAC 
CAGGCACAGG AGCAGCTGGG TCAGGTGCTG AAACGTCTCG GGAACATTCG AAACGAGATG 
GGCGTTTTCT ACATGAACCA GGCTGCAGCA ATGCAGGCAG AGAAAGAAGG AGGTCGTAAA 
AAAGACTGTA CAAATGCTAA GAAAACCAGG AGTAAAAGTA TAATTTTCTT TTCTGGTCCG 
GACCAGAAGA ACCCAGAAAA CCAAGCTACA ATGCGCAGGA GTGTGTGTGT GGCCGAGCAG 
GAGCTGTGGA AGAAGAGCTT CTCGTGTTTT GAGAAGGGCA TGCAGTATTT TAGTGCTATT 
TCAGACTCGG TGAACACGGC CCTGCTGCTG TGTAACATCG GCCGTCTCAT GCGCATCTGT 
GCTCAGGCTC ACTGCGTCAT TACGTCAGAG CAGAGCAGAG GAGAGTTTTC ACCTGAAGAG 
ACTCTCTATT ACAACAAGGC CATAGATTAT TATCAGAGAG CGCTGAAGGC TCTGGGCTGC 
AGAGAGAGTC ACTCCGCCGT CTGGGACTCG GTCAACTGGG AACTGTCCAC AACCTATTTC 
ACACTGGCCA CACTCCTGCA GGATTACGCC CCACTGTCCC GCAAAGCACA GGAGCAGATC 
GAGAGGGAAG TGACCGAGAC CATGATGAAG TCTCTGAGAT ACTGTGACCT GCAGACTGAA 
TCCGCTCGCC AGCCGCTCTA CCAGTACAGA GCAGCCACAA TCCACCACAG ACTGGCTTCA 
ATGTACCACA GCTGCTACCG CAATCAGGTT GGAGATGAGA GTGATCGTAA GCAGCACAAA 
GCTCTGGCAG AGCAGCACTA CAGTAAAGCC GTGCGCCTCT TTGTGAATCT GAGTGATGCT 
CCGTGTGAAC TGCTCCGCAC ACTTCTGGAG AGAGTGGCCT TCGCTGAGTT CACCATGGCA 
GGTCAGAACA GCAGTGCTGT GAAACTGAAG ACTCTGACAG GAGCTTTGGA GATCATGTGT 
GAAACACGGC ATGCGTTCAG ACTCATTCAT AAAGAGCTGC TGGAGCAACA AGAAAATATA 
ACGAAGGCTG AAGAGAAACC TGAAGAAGTC CCTGAGGGTT CAGAGAGTTC CTCTTCCTCT 
GAGCTGAACA CAGAAGAGGT GCTGAAGCTA ATTGGCATCT TCGAGCCCAG TTTCTCCTTC 
CTGTTACTGC AAGTGATTAA ACTCAGCTCT GCAAGCAAAC GAAAACTCAG TGGTAAAGAA 
GAGGAGCAGG TGAAGATCTA CAAGAGCGTC TATTCTCGTC TCTTACGGGC TGAAAAGTCT 
GATCCACTCA TTCAGCGGGT TTCGCTTTTC CTGGAGCTCC TGGATCAGCT GAATCCACCA 
TCTGGAAGTG AAGAACCAAG ATCATCATCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

edrf1 ( XM_689672.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_694764.4 Danio rerio erythroid differentiation regulatory factor 1 (edrf1), mRNA.

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CloneID	ODa22247
Clone ID Related Accession (Same CDS sequence)	XM_689672.8 , XM_021473083.1 , XM_689672.9
Accession Version	XM_689672.8	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	erythroid differentiation-related factor 1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_689672.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGC TGAATATGAG CTCTCAGGAT AAGACAGACA CAGATCTTAG ACAGGAGGAG 
GAACACAGCA GCTCTGAGGA CAACAAACAG GAGGGGGTTA TTTGTCTGGG CAACAATGAA 
GTGAAGAGTC GTGCAGTAGT GAAGTATTCG GCTGCTCCTC CTCCCTCCTC CTATGCATTA 
CTACAAGAGA AGACCGACCT CAAGCTGCCA CCAGCAAACT GGCTGCGCGA AAATGCTCAG 
CTGGGACCCG CAGGGACCAC CATACTCGGG TCCAGCCGGA AAAGCAAACC CTTCTCCAGT 
TTTGGGATGG CGTTTGACTT CATTGACTGT ATAGGTGATG ATGTCGATGT TGTGTCTGAT 
TCAGAGAACA TCAAGAAGCT TCTGAAGATC CCGTACAGTA AATCTCATGT CAGCATGGCT 
GTTCATAGAG TTGGCAGCAC TTTACTGTTG GACGAACTGG ACATTCAGGA GCTCTTCATG 
AGGTCATCAC AGACTAATGA CTGGACGTGG CTGAAGGAGT TTTACCAGCG TCTTATAGAC 
CAAAAGTGGC AGAGAAAGAA GAAAAGTAAA GAGCACTGGA ACGAGAAGGC CATCCTCTCC 
AAATTCCTCT ATTACAGTAT AAACAGTGAT GGGACTGCAG TGTCTGTGCC GTCAGATACA 
GAGCAAACAG GAGACGAGGG GTGTGGGGCA GGAACTGATG GACCCTCTTG GCCTGCGACT 
TTCAGCAGCT CCACCACTGA TTCAGAAGAG TCTGCACTGC CCAAAGAGGA GCAGGTGGAC 
ACATATGCTT TAGGTCACGT GTCGTCGGTT CCCAAAGAAC AGAATCTACC CACGCTGTTT 
AATGAAGGAG AAAACAGTCA GGGTTTGAGA AATGACTTTG TCCGAAATAT TTTATGGACA 
TTTGAGGACA TCCACATGCT TGTGGGATCA AATATGCCAA TTTTCGGAGG TGGACGATAC 
CCAGCAGTCA GTTTACGTCT CAGGGACAAC AACAAACCAA TCAACGTTCT CACAGGAATT 
GACTACTGGC TGGATAATTT GATGTGTAAC GTACCAGAGC TGGTCATGTG CTTTCACGTC 
AATGGCATTG TTCAGAAATA TGAAATGATA AAAACAGAGG ACATCCCCAA TTTAGAGAAC 
TCCACATTCT CCACGCGGGT TGTGAAAGAC ATTGCTCAAA ATATCCTGTC GTTCCTGAAA 
TCAAACTGTA CCAAAGAAGG ACACACGTAC TGGCTCTTCA AGGCCAGCGG GAGTGACATT 
GTGAAGCTGT ACGACCTCAC TACACTGTGT GAAGAAGCAG CAGAGGAGAA ATGCCAGAAT 
CCCTTCACAC TTCCAGTAGC CGTGCTTCTC TACAAAGTGG CCAGCAACAT GATGCTAAAG 
AAGAGCCAAA ACCGGAAGAT CTATGGGACA ATCAGAACTC TACTGCTCAA CTGTATTAAA 
CTGTTGGATG AGGACAGACA TCCACAGATC ATCGCTTCGG CTCACTACAT GCTGGCAGAA 
CTGTTTCAGC TGGATGATGT GAGTGAAGAC GAAGGCAATG GGGAGTCGCT GAGAGGAGGA 
GGATCTGAAG ACAGCTACAG TGATGAAGAT GAGGAGGATG AGGAGGAAGA TGAAGACGAG 
AGATGCTCCT TCAGCACTGC CTCTGAACCG CAGCACGACT CCAAAGCTGT GGCCGTCATC 
AAGTCTGTCG GAGAGCTTTC GGTTCCAGAG AAATACAAGA GCACGCATCA GATCAGACCA 
AACTTTGCAT ATCCTGTTTC ACAAGACAAG GAGGAGAAGT GCCGACTGGT TCTCAGCTAC 
GTTTTAAAAG GTCTGAAAGC AGTGGACAGC AGCATAAAGA AAGAGAGCGA CCTTCCTGCA 
GCCGATCCCA GCACACCCAT CCCACTAAAA TATGAGGAAA GAAGTCCAAA TGGAGCTAGA 
GAGGTGGAGC AGGAGATTGC TTTACTGCTG GATAGAGTTG GCCCATGTAA CGAAGAGTTG 
AAGGTGCCGA CACGCTCCGG GATGTTACCA GGCACATGGC AGCATCGCAT GAAACTCCAG 
CTGTTCCTGA AAGCATCGAA AGCGTATTAC GTTTTGTCAG ATGCTGCGAC CAACCTACTG 
AAGTACGGCC GGGCACTGCG CTACATCAAA CTGGCTCTGC AGTGCCACGA TGCATACTGC 
TCCATTAGTG GCGGTCTCCA CGCACAGGTG CTTCTTTTCC ACTGCCAGTG TCTGTCTCTG 
TGTGGAGATG TTCAGCTCAT GTTGGCCCAG AATGCCTCAA ACAGAGCTGC GTATCTGGAG 
GAGTACAGCT ATCAGACCAA AGATGACCAG GAGATCCTCC ACTGCCTGCA CCGCGAGAGC 
TCCTGCCAAG CCTTCAGCAT GGCCACTGAT CTGGCCCTGG ATCCTGAGTA CCAGCTTATC 
GTCAGCTGCA AGTGTTATGA AGCCGCTCAT GATCTTTTGA CGTCAGGAGT CCTGAAAGAC 
CAGGCACAGG AGCAGCTGGG TCAGGTGCTG AAACGTCTCG GGAACATTCG AAACGAGATG 
GGCGTTTTCT ACATGAACCA GGCTGCAGCA ATGCAGGCAG AGAAAGAAGG AGTGCGCAGG 
AGTGTGTGTG TGGCCGAGCA GGAGCTGTGG AAGAAGAGCT TCTCGTGTTT TGAGAAGGGC 
ATGCAGTATT TTAGTGCTAT TTCAGACTCG GTGAACACGG CCCTGCTGCT GTGTAACATC 
GGCCGTCTCA TGCGCATCTG TGCTCAGGCT CACTGCGTCA TTACGTCAGA GCAGAGCAGA 
GGAGAGTTTT CACCTGAAGA GACTCTCTAT TACAACAAGG CCATAGATTA TTATCAGAGA 
GCGCTGAAGG CTCTGGGCTG CAGAGAGAGT CACTCCGCCG TCTGGGACTC GGTCAACTGG 
GAACTGTCCA CAACCTATTT CACACTGGCC ACACTCCTGC AGGATTACGC CCCACTGTCC 
CGCAAAGCAC AGGAGCAGAT CGAGAGGGAA GTGACCGAGA CCATGATGAA GTCTCTGAGA 
TACTGTGACC TGCAGACTGA ATCCGCTCGC CAGCCGCTCT ACCAGTACAG AGCAGCCACA 
ATCCACCACA GACTGGCTTC AATGTACCAC AGCTGCTACC GCAATCAGGT TGGAGATGAG 
AGTGATCGTA AGCAGCACAA AGCTCTGGCA GAGCAGCACT ACAGTAAAGC CGTGCGCCTC 
TTTGTGAATC TGAGTGATGC TCCGTGTGAA CTGCTCCGCA CACTTCTGGA GAGAGTGGCC 
TTCGCTGAGT TCACCATGGC AGGTCAGAAC AGCAGTGCTG TGAAACTGAA GACTCTGACA 
GGAGCTTTGG AGATCATGTG TGAAACACGG CATGCGTTCA GACTCATTCA TAAAGAGCTG 
CTGGAGCAAC AAGAAAATAT AACGAAGGCT GAAGAGAAAC CTGAAGAAGT CCCTGAGGGT 
TCAGAGAGTT CCTCTTCCTC TGAGCTGAAC ACAGAAGAGG TGCTGAAGCT AATTGGCATC 
TTCGAGCCCA GTTTCTCCTT CCTGTTACTG CAAGTGATTA AACTCAGCTC TGCAAGCAAA 
CGAAAACTCA GTGGTAAAGA AGAGGAGCAG GTGAAGATCT ACAAGAGCGT CTATTCTCGT 
CTCTTACGGG CTGAAAAGTC TGATCCACTC ATTCAGCGGG TTTCGCTTTT CCTGGAGCTC 
CTGGATCAGC TGAATCCACC ATCTGGAAGT GAAGAACCAA GATCATCATC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_694764.4
CDS	364..4077
Translation	MSVLNMSSQDKTDTDLRQEEEHSSSEDNKQEGVICLGNNEVKSRAVVKYSAAPPPSSYALLQEKTDLKLPPANWLRENAQLGPAGTTILGSSRKSKPFSSFGMAFDFIDCIGDDVDVVSDSENIKKLLKIPYSKSHVSMAVHRVGSTLLLDELDIQELFMRSSQTNDWTWLKEFYQRLIDQKWQRKKKSKEHWNEKAILSKFLYYSINSDGTAVSVPSDTEQTGDEGCGAGTDGPSWPATFSSSTTDSEESALPKEEQVDTYALGHVSSVPKEQNLPTLFNEGENSQGLRNDFVRNILWTFEDIHMLVGSNMPIFGGGRYPAVSLRLRDNNKPINVLTGIDYWLDNLMCNVPELVMCFHVNGIVQKYEMIKTEDIPNLENSTFSTRVVKDIAQNILSFLKSNCTKEGHTYWLFKASGSDIVKLYDLTTLCEEAAEEKCQNPFTLPVAVLLYKVASNMMLKKSQNRKIYGTIRTLLLNCIKLLDEDRHPQIIASAHYMLAELFQLDDVSEDEGNGESLRGGGSEDSYSDEDEEDEEEDEDERCSFSTASEPQHDSKAVAVIKSVGELSVPEKYKSTHQIRPNFAYPVSQDKEEKCRLVLSYVLKGLKAVDSSIKKESDLPAADPSTPIPLKYEERSPNGAREVEQEIALLLDRVGPCNEELKVPTRSGMLPGTWQHRMKLQLFLKASKAYYVLSDAATNLLKYGRALRYIKLALQCHDAYCSISGGLHAQVLLFHCQCLSLCGDVQLMLAQNASNRAAYLEEYSYQTKDDQEILHCLHRESSCQAFSMATDLALDPEYQLIVSCKCYEAAHDLLTSGVLKDQAQEQLGQVLKRLGNIRNEMGVFYMNQAAAMQAEKEGVRRSVCVAEQELWKKSFSCFEKGMQYFSAISDSVNTALLLCNIGRLMRICAQAHCVITSEQSRGEFSPEETLYYNKAIDYYQRALKALGCRESHSAVWDSVNWELSTTYFTLATLLQDYAPLSRKAQEQIEREVTETMMKSLRYCDLQTESARQPLYQYRAATIHHRLASMYHSCYRNQVGDESDRKQHKALAEQHYSKAVRLFVNLSDAPCELLRTLLERVAFAEFTMAGQNSSAVKLKTLTGALEIMCETRHAFRLIHKELLEQQENITKAEEKPEEVPEGSESSSSSELNTEEVLKLIGIFEPSFSFLLLQVIKLSSASKRKLSGKEEEQVKIYKSVYSRLLRAEKSDPLIQRVSLFLELLDQLNPPSGSEEPRSSS

Target ORF information:

RefSeq Version	XM_689672.8
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio erythroid differentiation regulatory factor 1 (edrf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_689672.8

ORF Insert Sequence:

ATGAGTGTGC TGAATATGAG CTCTCAGGAT AAGACAGACA CAGATCTTAG ACAGGAGGAG 
GAACACAGCA GCTCTGAGGA CAACAAACAG GAGGGGGTTA TTTGTCTGGG CAACAATGAA 
GTGAAGAGTC GTGCAGTAGT GAAGTATTCG GCTGCTCCTC CTCCCTCCTC CTATGCATTA 
CTACAAGAGA AGACCGACCT CAAGCTGCCA CCAGCAAACT GGCTGCGCGA AAATGCTCAG 
CTGGGACCCG CAGGGACCAC CATACTCGGG TCCAGCCGGA AAAGCAAACC CTTCTCCAGT 
TTTGGGATGG CGTTTGACTT CATTGACTGT ATAGGTGATG ATGTCGATGT TGTGTCTGAT 
TCAGAGAACA TCAAGAAGCT TCTGAAGATC CCGTACAGTA AATCTCATGT CAGCATGGCT 
GTTCATAGAG TTGGCAGCAC TTTACTGTTG GACGAACTGG ACATTCAGGA GCTCTTCATG 
AGGTCATCAC AGACTAATGA CTGGACGTGG CTGAAGGAGT TTTACCAGCG TCTTATAGAC 
CAAAAGTGGC AGAGAAAGAA GAAAAGTAAA GAGCACTGGA ACGAGAAGGC CATCCTCTCC 
AAATTCCTCT ATTACAGTAT AAACAGTGAT GGGACTGCAG TGTCTGTGCC GTCAGATACA 
GAGCAAACAG GAGACGAGGG GTGTGGGGCA GGAACTGATG GACCCTCTTG GCCTGCGACT 
TTCAGCAGCT CCACCACTGA TTCAGAAGAG TCTGCACTGC CCAAAGAGGA GCAGGTGGAC 
ACATATGCTT TAGGTCACGT GTCGTCGGTT CCCAAAGAAC AGAATCTACC CACGCTGTTT 
AATGAAGGAG AAAACAGTCA GGGTTTGAGA AATGACTTTG TCCGAAATAT TTTATGGACA 
TTTGAGGACA TCCACATGCT TGTGGGATCA AATATGCCAA TTTTCGGAGG TGGACGATAC 
CCAGCAGTCA GTTTACGTCT CAGGGACAAC AACAAACCAA TCAACGTTCT CACAGGAATT 
GACTACTGGC TGGATAATTT GATGTGTAAC GTACCAGAGC TGGTCATGTG CTTTCACGTC 
AATGGCATTG TTCAGAAATA TGAAATGATA AAAACAGAGG ACATCCCCAA TTTAGAGAAC 
TCCACATTCT CCACGCGGGT TGTGAAAGAC ATTGCTCAAA ATATCCTGTC GTTCCTGAAA 
TCAAACTGTA CCAAAGAAGG ACACACGTAC TGGCTCTTCA AGGCCAGCGG GAGTGACATT 
GTGAAGCTGT ACGACCTCAC TACACTGTGT GAAGAAGCAG CAGAGGAGAA ATGCCAGAAT 
CCCTTCACAC TTCCAGTAGC CGTGCTTCTC TACAAAGTGG CCAGCAACAT GATGCTAAAG 
AAGAGCCAAA ACCGGAAGAT CTATGGGACA ATCAGAACTC TACTGCTCAA CTGTATTAAA 
CTGTTGGATG AGGACAGACA TCCACAGATC ATCGCTTCGG CTCACTACAT GCTGGCAGAA 
CTGTTTCAGC TGGATGATGT GAGTGAAGAC GAAGGCAATG GGGAGTCGCT GAGAGGAGGA 
GGATCTGAAG ACAGCTACAG TGATGAAGAT GAGGAGGATG AGGAGGAAGA TGAAGACGAG 
AGATGCTCCT TCAGCACTGC CTCTGAACCG CAGCACGACT CCAAAGCTGT GGCCGTCATC 
AAGTCTGTCG GAGAGCTTTC GGTTCCAGAG AAATACAAGA GCACGCATCA GATCAGACCA 
AACTTTGCAT ATCCTGTTTC ACAAGACAAG GAGGAGAAGT GCCGACTGGT TCTCAGCTAC 
GTTTTAAAAG GTCTGAAAGC AGTGGACAGC AGCATAAAGA AAGAGAGCGA CCTTCCTGCA 
GCCGATCCCA GCACACCCAT CCCACTAAAA TATGAGGAAA GAAGTCCAAA TGGAGCTAGA 
GAGGTGGAGC AGGAGATTGC TTTACTGCTG GATAGAGTTG GCCCATGTAA CGAAGAGTTG 
AAGGTGCCGA CACGCTCCGG GATGTTACCA GGCACATGGC AGCATCGCAT GAAACTCCAG 
CTGTTCCTGA AAGCATCGAA AGCGTATTAC GTTTTGTCAG ATGCTGCGAC CAACCTACTG 
AAGTACGGCC GGGCACTGCG CTACATCAAA CTGGCTCTGC AGTGCCACGA TGCATACTGC 
TCCATTAGTG GCGGTCTCCA CGCACAGGTG CTTCTTTTCC ACTGCCAGTG TCTGTCTCTG 
TGTGGAGATG TTCAGCTCAT GTTGGCCCAG AATGCCTCAA ACAGAGCTGC GTATCTGGAG 
GAGTACAGCT ATCAGACCAA AGATGACCAG GAGATCCTCC ACTGCCTGCA CCGCGAGAGC 
TCCTGCCAAG CCTTCAGCAT GGCCACTGAT CTGGCCCTGG ATCCTGAGTA CCAGCTTATC 
GTCAGCTGCA AGTGTTATGA AGCCGCTCAT GATCTTTTGA CGTCAGGAGT CCTGAAAGAC 
CAGGCACAGG AGCAGCTGGG TCAGGTGCTG AAACGTCTCG GGAACATTCG AAACGAGATG 
GGCGTTTTCT ACATGAACCA GGCTGCAGCA ATGCAGGCAG AGAAAGAAGG AGTGCGCAGG 
AGTGTGTGTG TGGCCGAGCA GGAGCTGTGG AAGAAGAGCT TCTCGTGTTT TGAGAAGGGC 
ATGCAGTATT TTAGTGCTAT TTCAGACTCG GTGAACACGG CCCTGCTGCT GTGTAACATC 
GGCCGTCTCA TGCGCATCTG TGCTCAGGCT CACTGCGTCA TTACGTCAGA GCAGAGCAGA 
GGAGAGTTTT CACCTGAAGA GACTCTCTAT TACAACAAGG CCATAGATTA TTATCAGAGA 
GCGCTGAAGG CTCTGGGCTG CAGAGAGAGT CACTCCGCCG TCTGGGACTC GGTCAACTGG 
GAACTGTCCA CAACCTATTT CACACTGGCC ACACTCCTGC AGGATTACGC CCCACTGTCC 
CGCAAAGCAC AGGAGCAGAT CGAGAGGGAA GTGACCGAGA CCATGATGAA GTCTCTGAGA 
TACTGTGACC TGCAGACTGA ATCCGCTCGC CAGCCGCTCT ACCAGTACAG AGCAGCCACA 
ATCCACCACA GACTGGCTTC AATGTACCAC AGCTGCTACC GCAATCAGGT TGGAGATGAG 
AGTGATCGTA AGCAGCACAA AGCTCTGGCA GAGCAGCACT ACAGTAAAGC CGTGCGCCTC 
TTTGTGAATC TGAGTGATGC TCCGTGTGAA CTGCTCCGCA CACTTCTGGA GAGAGTGGCC 
TTCGCTGAGT TCACCATGGC AGGTCAGAAC AGCAGTGCTG TGAAACTGAA GACTCTGACA 
GGAGCTTTGG AGATCATGTG TGAAACACGG CATGCGTTCA GACTCATTCA TAAAGAGCTG 
CTGGAGCAAC AAGAAAATAT AACGAAGGCT GAAGAGAAAC CTGAAGAAGT CCCTGAGGGT 
TCAGAGAGTT CCTCTTCCTC TGAGCTGAAC ACAGAAGAGG TGCTGAAGCT AATTGGCATC 
TTCGAGCCCA GTTTCTCCTT CCTGTTACTG CAAGTGATTA AACTCAGCTC TGCAAGCAAA 
CGAAAACTCA GTGGTAAAGA AGAGGAGCAG GTGAAGATCT ACAAGAGCGT CTATTCTCGT 
CTCTTACGGG CTGAAAAGTC TGATCCACTC ATTCAGCGGG TTTCGCTTTT CCTGGAGCTC 
CTGGATCAGC TGAATCCACC ATCTGGAAGT GAAGAACCAA GATCATCATC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

edrf1 ( XM_021473083.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328758.1 Danio rerio erythroid differentiation regulatory factor 1 (edrf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa22247
Clone ID Related Accession (Same CDS sequence)	XM_689672.8 , XM_021473083.1 , XM_689672.9
Accession Version	XM_021473083.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	erythroid differentiation-related factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394977.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGC TGAATATGAG CTCTCAGGAT AAGACAGACA CAGATCTTAG ACAGGAGGAG 
GAACACAGCA GCTCTGAGGA CAACAAACAG GAGGGGGTTA TTTGTCTGGG CAACAATGAA 
GTGAAGAGTC GTGCAGTAGT GAAGTATTCG GCTGCTCCTC CTCCCTCCTC CTATGCATTA 
CTACAAGAGA AGACCGACCT CAAGCTGCCA CCAGCAAACT GGCTGCGCGA AAATGCTCAG 
CTGGGACCCG CAGGGACCAC CATACTCGGG TCCAGCCGGA AAAGCAAACC CTTCTCCAGT 
TTTGGGATGG CGTTTGACTT CATTGACTGT ATAGGTGATG ATGTCGATGT TGTGTCTGAT 
TCAGAGAACA TCAAGAAGCT TCTGAAGATC CCGTACAGTA AATCTCATGT CAGCATGGCT 
GTTCATAGAG TTGGCAGCAC TTTACTGTTG GACGAACTGG ACATTCAGGA GCTCTTCATG 
AGGTCATCAC AGACTAATGA CTGGACGTGG CTGAAGGAGT TTTACCAGCG TCTTATAGAC 
CAAAAGTGGC AGAGAAAGAA GAAAAGTAAA GAGCACTGGA ACGAGAAGGC CATCCTCTCC 
AAATTCCTCT ATTACAGTAT AAACAGTGAT GGGACTGCAG TGTCTGTGCC GTCAGATACA 
GAGCAAACAG GAGACGAGGG GTGTGGGGCA GGAACTGATG GACCCTCTTG GCCTGCGACT 
TTCAGCAGCT CCACCACTGA TTCAGAAGAG TCTGCACTGC CCAAAGAGGA GCAGGTGGAC 
ACATATGCTT TAGGTCACGT GTCGTCGGTT CCCAAAGAAC AGAATCTACC CACGCTGTTT 
AATGAAGGAG AAAACAGTCA GGGTTTGAGA AATGACTTTG TCCGAAATAT TTTATGGACA 
TTTGAGGACA TCCACATGCT TGTGGGATCA AATATGCCAA TTTTCGGAGG TGGACGATAC 
CCAGCAGTCA GTTTACGTCT CAGGGACAAC AACAAACCAA TCAACGTTCT CACAGGAATT 
GACTACTGGC TGGATAATTT GATGTGTAAC GTACCAGAGC TGGTCATGTG CTTTCACGTC 
AATGGCATTG TTCAGAAATA TGAAATGATA AAAACAGAGG ACATCCCCAA TTTAGAGAAC 
TCCACATTCT CCACGCGGGT TGTGAAAGAC ATTGCTCAAA ATATCCTGTC GTTCCTGAAA 
TCAAACTGTA CCAAAGAAGG ACACACGTAC TGGCTCTTCA AGGCCAGCGG GAGTGACATT 
GTGAAGCTGT ACGACCTCAC TACACTGTGT GAAGAAGCAG CAGAGGAGAA ATGCCAGAAT 
CCCTTCACAC TTCCAGTAGC CGTGCTTCTC TACAAAGTGG CCAGCAACAT GATGCTAAAG 
AAGAGCCAAA ACCGGAAGAT CTATGGGACA ATCAGAACTC TACTGCTCAA CTGTATTAAA 
CTGTTGGATG AGGACAGACA TCCACAGATC ATCGCTTCGG CTCACTACAT GCTGGCAGAA 
CTGTTTCAGC TGGATGATGT GAGTGAAGAC GAAGGCAATG GGGAGTCGCT GAGAGGAGGA 
GGATCTGAAG ACAGCTACAG TGATGAAGAT GAGGAGGATG AGGAGGAAGA TGAAGACGAG 
AGATGCTCCT TCAGCACTGC CTCTGAACCG CAGCACGACT CCAAAGCTGT GGCCGTCATC 
AAGTCTGTCG GAGAGCTTTC GGTTCCAGAG AAATACAAGA GCACGCATCA GATCAGACCA 
AACTTTGCAT ATCCTGTTTC ACAAGACAAG GAGGAGAAGT GCCGACTGGT TCTCAGCTAC 
GTTTTAAAAG GTCTGAAAGC AGTGGACAGC AGCATAAAGA AAGAGAGCGA CCTTCCTGCA 
GCCGATCCCA GCACACCCAT CCCACTAAAA TATGAGGAAA GAAGTCCAAA TGGAGCTAGA 
GAGGTGGAGC AGGAGATTGC TTTACTGCTG GATAGAGTTG GCCCATGTAA CGAAGAGTTG 
AAGGTGCCGA CACGCTCCGG GATGTTACCA GGCACATGGC AGCATCGCAT GAAACTCCAG 
CTGTTCCTGA AAGCATCGAA AGCGTATTAC GTTTTGTCAG ATGCTGCGAC CAACCTACTG 
AAGTACGGCC GGGCACTGCG CTACATCAAA CTGGCTCTGC AGTGCCACGA TGCATACTGC 
TCCATTAGTG GCGGTCTCCA CGCACAGGTG CTTCTTTTCC ACTGCCAGTG TCTGTCTCTG 
TGTGGAGATG TTCAGCTCAT GTTGGCCCAG AATGCCTCAA ACAGAGCTGC GTATCTGGAG 
GAGTACAGCT ATCAGACCAA AGATGACCAG GAGATCCTCC ACTGCCTGCA CCGCGAGAGC 
TCCTGCCAAG CCTTCAGCAT GGCCACTGAT CTGGCCCTGG ATCCTGAGTA CCAGCTTATC 
GTCAGCTGCA AGTGTTATGA AGCCGCTCAT GATCTTTTGA CGTCAGGAGT CCTGAAAGAC 
CAGGCACAGG AGCAGCTGGG TCAGGTGCTG AAACGTCTCG GGAACATTCG AAACGAGATG 
GGCGTTTTCT ACATGAACCA GGCTGCAGCA ATGCAGGCAG AGAAAGAAGG AGTGCGCAGG 
AGTGTGTGTG TGGCCGAGCA GGAGCTGTGG AAGAAGAGCT TCTCGTGTTT TGAGAAGGGC 
ATGCAGTATT TTAGTGCTAT TTCAGACTCG GTGAACACGG CCCTGCTGCT GTGTAACATC 
GGCCGTCTCA TGCGCATCTG TGCTCAGGCT CACTGCGTCA TTACGTCAGA GCAGAGCAGA 
GGAGAGTTTT CACCTGAAGA GACTCTCTAT TACAACAAGG CCATAGATTA TTATCAGAGA 
GCGCTGAAGG CTCTGGGCTG CAGAGAGAGT CACTCCGCCG TCTGGGACTC GGTCAACTGG 
GAACTGTCCA CAACCTATTT CACACTGGCC ACACTCCTGC AGGATTACGC CCCACTGTCC 
CGCAAAGCAC AGGAGCAGAT CGAGAGGGAA GTGACCGAGA CCATGATGAA GTCTCTGAGA 
TACTGTGACC TGCAGACTGA ATCCGCTCGC CAGCCGCTCT ACCAGTACAG AGCAGCCACA 
ATCCACCACA GACTGGCTTC AATGTACCAC AGCTGCTACC GCAATCAGGT TGGAGATGAG 
AGTGATCGTA AGCAGCACAA AGCTCTGGCA GAGCAGCACT ACAGTAAAGC CGTGCGCCTC 
TTTGTGAATC TGAGTGATGC TCCGTGTGAA CTGCTCCGCA CACTTCTGGA GAGAGTGGCC 
TTCGCTGAGT TCACCATGGC AGGTCAGAAC AGCAGTGCTG TGAAACTGAA GACTCTGACA 
GGAGCTTTGG AGATCATGTG TGAAACACGG CATGCGTTCA GACTCATTCA TAAAGAGCTG 
CTGGAGCAAC AAGAAAATAT AACGAAGGCT GAAGAGAAAC CTGAAGAAGT CCCTGAGGGT 
TCAGAGAGTT CCTCTTCCTC TGAGCTGAAC ACAGAAGAGG TGCTGAAGCT AATTGGCATC 
TTCGAGCCCA GTTTCTCCTT CCTGTTACTG CAAGTGATTA AACTCAGCTC TGCAAGCAAA 
CGAAAACTCA GTGGTAAAGA AGAGGAGCAG GTGAAGATCT ACAAGAGCGT CTATTCTCGT 
CTCTTACGGG CTGAAAAGTC TGATCCACTC ATTCAGCGGG TTTCGCTTTT CCTGGAGCTC 
CTGGATCAGC TGAATCCACC ATCTGGAAGT GAAGAACCAA GATCATCATC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328758.1
CDS	370..4083
Translation	MSVLNMSSQDKTDTDLRQEEEHSSSEDNKQEGVICLGNNEVKSRAVVKYSAAPPPSSYALLQEKTDLKLPPANWLRENAQLGPAGTTILGSSRKSKPFSSFGMAFDFIDCIGDDVDVVSDSENIKKLLKIPYSKSHVSMAVHRVGSTLLLDELDIQELFMRSSQTNDWTWLKEFYQRLIDQKWQRKKKSKEHWNEKAILSKFLYYSINSDGTAVSVPSDTEQTGDEGCGAGTDGPSWPATFSSSTTDSEESALPKEEQVDTYALGHVSSVPKEQNLPTLFNEGENSQGLRNDFVRNILWTFEDIHMLVGSNMPIFGGGRYPAVSLRLRDNNKPINVLTGIDYWLDNLMCNVPELVMCFHVNGIVQKYEMIKTEDIPNLENSTFSTRVVKDIAQNILSFLKSNCTKEGHTYWLFKASGSDIVKLYDLTTLCEEAAEEKCQNPFTLPVAVLLYKVASNMMLKKSQNRKIYGTIRTLLLNCIKLLDEDRHPQIIASAHYMLAELFQLDDVSEDEGNGESLRGGGSEDSYSDEDEEDEEEDEDERCSFSTASEPQHDSKAVAVIKSVGELSVPEKYKSTHQIRPNFAYPVSQDKEEKCRLVLSYVLKGLKAVDSSIKKESDLPAADPSTPIPLKYEERSPNGAREVEQEIALLLDRVGPCNEELKVPTRSGMLPGTWQHRMKLQLFLKASKAYYVLSDAATNLLKYGRALRYIKLALQCHDAYCSISGGLHAQVLLFHCQCLSLCGDVQLMLAQNASNRAAYLEEYSYQTKDDQEILHCLHRESSCQAFSMATDLALDPEYQLIVSCKCYEAAHDLLTSGVLKDQAQEQLGQVLKRLGNIRNEMGVFYMNQAAAMQAEKEGVRRSVCVAEQELWKKSFSCFEKGMQYFSAISDSVNTALLLCNIGRLMRICAQAHCVITSEQSRGEFSPEETLYYNKAIDYYQRALKALGCRESHSAVWDSVNWELSTTYFTLATLLQDYAPLSRKAQEQIEREVTETMMKSLRYCDLQTESARQPLYQYRAATIHHRLASMYHSCYRNQVGDESDRKQHKALAEQHYSKAVRLFVNLSDAPCELLRTLLERVAFAEFTMAGQNSSAVKLKTLTGALEIMCETRHAFRLIHKELLEQQENITKAEEKPEEVPEGSESSSSSELNTEEVLKLIGIFEPSFSFLLLQVIKLSSASKRKLSGKEEEQVKIYKSVYSRLLRAEKSDPLIQRVSLFLELLDQLNPPSGSEEPRSSS

Target ORF information:

RefSeq Version	XM_021473083.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio erythroid differentiation regulatory factor 1 (edrf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473083.1

ORF Insert Sequence:

ATGAGTGTGC TGAATATGAG CTCTCAGGAT AAGACAGACA CAGATCTTAG ACAGGAGGAG 
GAACACAGCA GCTCTGAGGA CAACAAACAG GAGGGGGTTA TTTGTCTGGG CAACAATGAA 
GTGAAGAGTC GTGCAGTAGT GAAGTATTCG GCTGCTCCTC CTCCCTCCTC CTATGCATTA 
CTACAAGAGA AGACCGACCT CAAGCTGCCA CCAGCAAACT GGCTGCGCGA AAATGCTCAG 
CTGGGACCCG CAGGGACCAC CATACTCGGG TCCAGCCGGA AAAGCAAACC CTTCTCCAGT 
TTTGGGATGG CGTTTGACTT CATTGACTGT ATAGGTGATG ATGTCGATGT TGTGTCTGAT 
TCAGAGAACA TCAAGAAGCT TCTGAAGATC CCGTACAGTA AATCTCATGT CAGCATGGCT 
GTTCATAGAG TTGGCAGCAC TTTACTGTTG GACGAACTGG ACATTCAGGA GCTCTTCATG 
AGGTCATCAC AGACTAATGA CTGGACGTGG CTGAAGGAGT TTTACCAGCG TCTTATAGAC 
CAAAAGTGGC AGAGAAAGAA GAAAAGTAAA GAGCACTGGA ACGAGAAGGC CATCCTCTCC 
AAATTCCTCT ATTACAGTAT AAACAGTGAT GGGACTGCAG TGTCTGTGCC GTCAGATACA 
GAGCAAACAG GAGACGAGGG GTGTGGGGCA GGAACTGATG GACCCTCTTG GCCTGCGACT 
TTCAGCAGCT CCACCACTGA TTCAGAAGAG TCTGCACTGC CCAAAGAGGA GCAGGTGGAC 
ACATATGCTT TAGGTCACGT GTCGTCGGTT CCCAAAGAAC AGAATCTACC CACGCTGTTT 
AATGAAGGAG AAAACAGTCA GGGTTTGAGA AATGACTTTG TCCGAAATAT TTTATGGACA 
TTTGAGGACA TCCACATGCT TGTGGGATCA AATATGCCAA TTTTCGGAGG TGGACGATAC 
CCAGCAGTCA GTTTACGTCT CAGGGACAAC AACAAACCAA TCAACGTTCT CACAGGAATT 
GACTACTGGC TGGATAATTT GATGTGTAAC GTACCAGAGC TGGTCATGTG CTTTCACGTC 
AATGGCATTG TTCAGAAATA TGAAATGATA AAAACAGAGG ACATCCCCAA TTTAGAGAAC 
TCCACATTCT CCACGCGGGT TGTGAAAGAC ATTGCTCAAA ATATCCTGTC GTTCCTGAAA 
TCAAACTGTA CCAAAGAAGG ACACACGTAC TGGCTCTTCA AGGCCAGCGG GAGTGACATT 
GTGAAGCTGT ACGACCTCAC TACACTGTGT GAAGAAGCAG CAGAGGAGAA ATGCCAGAAT 
CCCTTCACAC TTCCAGTAGC CGTGCTTCTC TACAAAGTGG CCAGCAACAT GATGCTAAAG 
AAGAGCCAAA ACCGGAAGAT CTATGGGACA ATCAGAACTC TACTGCTCAA CTGTATTAAA 
CTGTTGGATG AGGACAGACA TCCACAGATC ATCGCTTCGG CTCACTACAT GCTGGCAGAA 
CTGTTTCAGC TGGATGATGT GAGTGAAGAC GAAGGCAATG GGGAGTCGCT GAGAGGAGGA 
GGATCTGAAG ACAGCTACAG TGATGAAGAT GAGGAGGATG AGGAGGAAGA TGAAGACGAG 
AGATGCTCCT TCAGCACTGC CTCTGAACCG CAGCACGACT CCAAAGCTGT GGCCGTCATC 
AAGTCTGTCG GAGAGCTTTC GGTTCCAGAG AAATACAAGA GCACGCATCA GATCAGACCA 
AACTTTGCAT ATCCTGTTTC ACAAGACAAG GAGGAGAAGT GCCGACTGGT TCTCAGCTAC 
GTTTTAAAAG GTCTGAAAGC AGTGGACAGC AGCATAAAGA AAGAGAGCGA CCTTCCTGCA 
GCCGATCCCA GCACACCCAT CCCACTAAAA TATGAGGAAA GAAGTCCAAA TGGAGCTAGA 
GAGGTGGAGC AGGAGATTGC TTTACTGCTG GATAGAGTTG GCCCATGTAA CGAAGAGTTG 
AAGGTGCCGA CACGCTCCGG GATGTTACCA GGCACATGGC AGCATCGCAT GAAACTCCAG 
CTGTTCCTGA AAGCATCGAA AGCGTATTAC GTTTTGTCAG ATGCTGCGAC CAACCTACTG 
AAGTACGGCC GGGCACTGCG CTACATCAAA CTGGCTCTGC AGTGCCACGA TGCATACTGC 
TCCATTAGTG GCGGTCTCCA CGCACAGGTG CTTCTTTTCC ACTGCCAGTG TCTGTCTCTG 
TGTGGAGATG TTCAGCTCAT GTTGGCCCAG AATGCCTCAA ACAGAGCTGC GTATCTGGAG 
GAGTACAGCT ATCAGACCAA AGATGACCAG GAGATCCTCC ACTGCCTGCA CCGCGAGAGC 
TCCTGCCAAG CCTTCAGCAT GGCCACTGAT CTGGCCCTGG ATCCTGAGTA CCAGCTTATC 
GTCAGCTGCA AGTGTTATGA AGCCGCTCAT GATCTTTTGA CGTCAGGAGT CCTGAAAGAC 
CAGGCACAGG AGCAGCTGGG TCAGGTGCTG AAACGTCTCG GGAACATTCG AAACGAGATG 
GGCGTTTTCT ACATGAACCA GGCTGCAGCA ATGCAGGCAG AGAAAGAAGG AGTGCGCAGG 
AGTGTGTGTG TGGCCGAGCA GGAGCTGTGG AAGAAGAGCT TCTCGTGTTT TGAGAAGGGC 
ATGCAGTATT TTAGTGCTAT TTCAGACTCG GTGAACACGG CCCTGCTGCT GTGTAACATC 
GGCCGTCTCA TGCGCATCTG TGCTCAGGCT CACTGCGTCA TTACGTCAGA GCAGAGCAGA 
GGAGAGTTTT CACCTGAAGA GACTCTCTAT TACAACAAGG CCATAGATTA TTATCAGAGA 
GCGCTGAAGG CTCTGGGCTG CAGAGAGAGT CACTCCGCCG TCTGGGACTC GGTCAACTGG 
GAACTGTCCA CAACCTATTT CACACTGGCC ACACTCCTGC AGGATTACGC CCCACTGTCC 
CGCAAAGCAC AGGAGCAGAT CGAGAGGGAA GTGACCGAGA CCATGATGAA GTCTCTGAGA 
TACTGTGACC TGCAGACTGA ATCCGCTCGC CAGCCGCTCT ACCAGTACAG AGCAGCCACA 
ATCCACCACA GACTGGCTTC AATGTACCAC AGCTGCTACC GCAATCAGGT TGGAGATGAG 
AGTGATCGTA AGCAGCACAA AGCTCTGGCA GAGCAGCACT ACAGTAAAGC CGTGCGCCTC 
TTTGTGAATC TGAGTGATGC TCCGTGTGAA CTGCTCCGCA CACTTCTGGA GAGAGTGGCC 
TTCGCTGAGT TCACCATGGC AGGTCAGAAC AGCAGTGCTG TGAAACTGAA GACTCTGACA 
GGAGCTTTGG AGATCATGTG TGAAACACGG CATGCGTTCA GACTCATTCA TAAAGAGCTG 
CTGGAGCAAC AAGAAAATAT AACGAAGGCT GAAGAGAAAC CTGAAGAAGT CCCTGAGGGT 
TCAGAGAGTT CCTCTTCCTC TGAGCTGAAC ACAGAAGAGG TGCTGAAGCT AATTGGCATC 
TTCGAGCCCA GTTTCTCCTT CCTGTTACTG CAAGTGATTA AACTCAGCTC TGCAAGCAAA 
CGAAAACTCA GTGGTAAAGA AGAGGAGCAG GTGAAGATCT ACAAGAGCGT CTATTCTCGT 
CTCTTACGGG CTGAAAAGTC TGATCCACTC ATTCAGCGGG TTTCGCTTTT CCTGGAGCTC 
CTGGATCAGC TGAATCCACC ATCTGGAAGT GAAGAACCAA GATCATCATC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

edrf1 ( XM_689672.9 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_694764.4 Danio rerio erythroid differentiation regulatory factor 1 (edrf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa22247
Clone ID Related Accession (Same CDS sequence)	XM_689672.8 , XM_021473083.1 , XM_689672.9
Accession Version	XM_689672.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3714bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	erythroid differentiation-related factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_689672.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGTGC TGAATATGAG CTCTCAGGAT AAGACAGACA CAGATCTTAG ACAGGAGGAG 
GAACACAGCA GCTCTGAGGA CAACAAACAG GAGGGGGTTA TTTGTCTGGG CAACAATGAA 
GTGAAGAGTC GTGCAGTAGT GAAGTATTCG GCTGCTCCTC CTCCCTCCTC CTATGCATTA 
CTACAAGAGA AGACCGACCT CAAGCTGCCA CCAGCAAACT GGCTGCGCGA AAATGCTCAG 
CTGGGACCCG CAGGGACCAC CATACTCGGG TCCAGCCGGA AAAGCAAACC CTTCTCCAGT 
TTTGGGATGG CGTTTGACTT CATTGACTGT ATAGGTGATG ATGTCGATGT TGTGTCTGAT 
TCAGAGAACA TCAAGAAGCT TCTGAAGATC CCGTACAGTA AATCTCATGT CAGCATGGCT 
GTTCATAGAG TTGGCAGCAC TTTACTGTTG GACGAACTGG ACATTCAGGA GCTCTTCATG 
AGGTCATCAC AGACTAATGA CTGGACGTGG CTGAAGGAGT TTTACCAGCG TCTTATAGAC 
CAAAAGTGGC AGAGAAAGAA GAAAAGTAAA GAGCACTGGA ACGAGAAGGC CATCCTCTCC 
AAATTCCTCT ATTACAGTAT AAACAGTGAT GGGACTGCAG TGTCTGTGCC GTCAGATACA 
GAGCAAACAG GAGACGAGGG GTGTGGGGCA GGAACTGATG GACCCTCTTG GCCTGCGACT 
TTCAGCAGCT CCACCACTGA TTCAGAAGAG TCTGCACTGC CCAAAGAGGA GCAGGTGGAC 
ACATATGCTT TAGGTCACGT GTCGTCGGTT CCCAAAGAAC AGAATCTACC CACGCTGTTT 
AATGAAGGAG AAAACAGTCA GGGTTTGAGA AATGACTTTG TCCGAAATAT TTTATGGACA 
TTTGAGGACA TCCACATGCT TGTGGGATCA AATATGCCAA TTTTCGGAGG TGGACGATAC 
CCAGCAGTCA GTTTACGTCT CAGGGACAAC AACAAACCAA TCAACGTTCT CACAGGAATT 
GACTACTGGC TGGATAATTT GATGTGTAAC GTACCAGAGC TGGTCATGTG CTTTCACGTC 
AATGGCATTG TTCAGAAATA TGAAATGATA AAAACAGAGG ACATCCCCAA TTTAGAGAAC 
TCCACATTCT CCACGCGGGT TGTGAAAGAC ATTGCTCAAA ATATCCTGTC GTTCCTGAAA 
TCAAACTGTA CCAAAGAAGG ACACACGTAC TGGCTCTTCA AGGCCAGCGG GAGTGACATT 
GTGAAGCTGT ACGACCTCAC TACACTGTGT GAAGAAGCAG CAGAGGAGAA ATGCCAGAAT 
CCCTTCACAC TTCCAGTAGC CGTGCTTCTC TACAAAGTGG CCAGCAACAT GATGCTAAAG 
AAGAGCCAAA ACCGGAAGAT CTATGGGACA ATCAGAACTC TACTGCTCAA CTGTATTAAA 
CTGTTGGATG AGGACAGACA TCCACAGATC ATCGCTTCGG CTCACTACAT GCTGGCAGAA 
CTGTTTCAGC TGGATGATGT GAGTGAAGAC GAAGGCAATG GGGAGTCGCT GAGAGGAGGA 
GGATCTGAAG ACAGCTACAG TGATGAAGAT GAGGAGGATG AGGAGGAAGA TGAAGACGAG 
AGATGCTCCT TCAGCACTGC CTCTGAACCG CAGCACGACT CCAAAGCTGT GGCCGTCATC 
AAGTCTGTCG GAGAGCTTTC GGTTCCAGAG AAATACAAGA GCACGCATCA GATCAGACCA 
AACTTTGCAT ATCCTGTTTC ACAAGACAAG GAGGAGAAGT GCCGACTGGT TCTCAGCTAC 
GTTTTAAAAG GTCTGAAAGC AGTGGACAGC AGCATAAAGA AAGAGAGCGA CCTTCCTGCA 
GCCGATCCCA GCACACCCAT CCCACTAAAA TATGAGGAAA GAAGTCCAAA TGGAGCTAGA 
GAGGTGGAGC AGGAGATTGC TTTACTGCTG GATAGAGTTG GCCCATGTAA CGAAGAGTTG 
AAGGTGCCGA CACGCTCCGG GATGTTACCA GGCACATGGC AGCATCGCAT GAAACTCCAG 
CTGTTCCTGA AAGCATCGAA AGCGTATTAC GTTTTGTCAG ATGCTGCGAC CAACCTACTG 
AAGTACGGCC GGGCACTGCG CTACATCAAA CTGGCTCTGC AGTGCCACGA TGCATACTGC 
TCCATTAGTG GCGGTCTCCA CGCACAGGTG CTTCTTTTCC ACTGCCAGTG TCTGTCTCTG 
TGTGGAGATG TTCAGCTCAT GTTGGCCCAG AATGCCTCAA ACAGAGCTGC GTATCTGGAG 
GAGTACAGCT ATCAGACCAA AGATGACCAG GAGATCCTCC ACTGCCTGCA CCGCGAGAGC 
TCCTGCCAAG CCTTCAGCAT GGCCACTGAT CTGGCCCTGG ATCCTGAGTA CCAGCTTATC 
GTCAGCTGCA AGTGTTATGA AGCCGCTCAT GATCTTTTGA CGTCAGGAGT CCTGAAAGAC 
CAGGCACAGG AGCAGCTGGG TCAGGTGCTG AAACGTCTCG GGAACATTCG AAACGAGATG 
GGCGTTTTCT ACATGAACCA GGCTGCAGCA ATGCAGGCAG AGAAAGAAGG AGTGCGCAGG 
AGTGTGTGTG TGGCCGAGCA GGAGCTGTGG AAGAAGAGCT TCTCGTGTTT TGAGAAGGGC 
ATGCAGTATT TTAGTGCTAT TTCAGACTCG GTGAACACGG CCCTGCTGCT GTGTAACATC 
GGCCGTCTCA TGCGCATCTG TGCTCAGGCT CACTGCGTCA TTACGTCAGA GCAGAGCAGA 
GGAGAGTTTT CACCTGAAGA GACTCTCTAT TACAACAAGG CCATAGATTA TTATCAGAGA 
GCGCTGAAGG CTCTGGGCTG CAGAGAGAGT CACTCCGCCG TCTGGGACTC GGTCAACTGG 
GAACTGTCCA CAACCTATTT CACACTGGCC ACACTCCTGC AGGATTACGC CCCACTGTCC 
CGCAAAGCAC AGGAGCAGAT CGAGAGGGAA GTGACCGAGA CCATGATGAA GTCTCTGAGA 
TACTGTGACC TGCAGACTGA ATCCGCTCGC CAGCCGCTCT ACCAGTACAG AGCAGCCACA 
ATCCACCACA GACTGGCTTC AATGTACCAC AGCTGCTACC GCAATCAGGT TGGAGATGAG 
AGTGATCGTA AGCAGCACAA AGCTCTGGCA GAGCAGCACT ACAGTAAAGC CGTGCGCCTC 
TTTGTGAATC TGAGTGATGC TCCGTGTGAA CTGCTCCGCA CACTTCTGGA GAGAGTGGCC 
TTCGCTGAGT TCACCATGGC AGGTCAGAAC AGCAGTGCTG TGAAACTGAA GACTCTGACA 
GGAGCTTTGG AGATCATGTG TGAAACACGG CATGCGTTCA GACTCATTCA TAAAGAGCTG 
CTGGAGCAAC AAGAAAATAT AACGAAGGCT GAAGAGAAAC CTGAAGAAGT CCCTGAGGGT 
TCAGAGAGTT CCTCTTCCTC TGAGCTGAAC ACAGAAGAGG TGCTGAAGCT AATTGGCATC 
TTCGAGCCCA GTTTCTCCTT CCTGTTACTG CAAGTGATTA AACTCAGCTC TGCAAGCAAA 
CGAAAACTCA GTGGTAAAGA AGAGGAGCAG GTGAAGATCT ACAAGAGCGT CTATTCTCGT 
CTCTTACGGG CTGAAAAGTC TGATCCACTC ATTCAGCGGG TTTCGCTTTT CCTGGAGCTC 
CTGGATCAGC TGAATCCACC ATCTGGAAGT GAAGAACCAA GATCATCATC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_694764.4
CDS	370..4083
Translation	MSVLNMSSQDKTDTDLRQEEEHSSSEDNKQEGVICLGNNEVKSRAVVKYSAAPPPSSYALLQEKTDLKLPPANWLRENAQLGPAGTTILGSSRKSKPFSSFGMAFDFIDCIGDDVDVVSDSENIKKLLKIPYSKSHVSMAVHRVGSTLLLDELDIQELFMRSSQTNDWTWLKEFYQRLIDQKWQRKKKSKEHWNEKAILSKFLYYSINSDGTAVSVPSDTEQTGDEGCGAGTDGPSWPATFSSSTTDSEESALPKEEQVDTYALGHVSSVPKEQNLPTLFNEGENSQGLRNDFVRNILWTFEDIHMLVGSNMPIFGGGRYPAVSLRLRDNNKPINVLTGIDYWLDNLMCNVPELVMCFHVNGIVQKYEMIKTEDIPNLENSTFSTRVVKDIAQNILSFLKSNCTKEGHTYWLFKASGSDIVKLYDLTTLCEEAAEEKCQNPFTLPVAVLLYKVASNMMLKKSQNRKIYGTIRTLLLNCIKLLDEDRHPQIIASAHYMLAELFQLDDVSEDEGNGESLRGGGSEDSYSDEDEEDEEEDEDERCSFSTASEPQHDSKAVAVIKSVGELSVPEKYKSTHQIRPNFAYPVSQDKEEKCRLVLSYVLKGLKAVDSSIKKESDLPAADPSTPIPLKYEERSPNGAREVEQEIALLLDRVGPCNEELKVPTRSGMLPGTWQHRMKLQLFLKASKAYYVLSDAATNLLKYGRALRYIKLALQCHDAYCSISGGLHAQVLLFHCQCLSLCGDVQLMLAQNASNRAAYLEEYSYQTKDDQEILHCLHRESSCQAFSMATDLALDPEYQLIVSCKCYEAAHDLLTSGVLKDQAQEQLGQVLKRLGNIRNEMGVFYMNQAAAMQAEKEGVRRSVCVAEQELWKKSFSCFEKGMQYFSAISDSVNTALLLCNIGRLMRICAQAHCVITSEQSRGEFSPEETLYYNKAIDYYQRALKALGCRESHSAVWDSVNWELSTTYFTLATLLQDYAPLSRKAQEQIEREVTETMMKSLRYCDLQTESARQPLYQYRAATIHHRLASMYHSCYRNQVGDESDRKQHKALAEQHYSKAVRLFVNLSDAPCELLRTLLERVAFAEFTMAGQNSSAVKLKTLTGALEIMCETRHAFRLIHKELLEQQENITKAEEKPEEVPEGSESSSSSELNTEEVLKLIGIFEPSFSFLLLQVIKLSSASKRKLSGKEEEQVKIYKSVYSRLLRAEKSDPLIQRVSLFLELLDQLNPPSGSEEPRSSS

Target ORF information:

RefSeq Version	XM_689672.9
Organism	Danio rerio(zebrafish)
Definition	Danio rerio erythroid differentiation regulatory factor 1 (edrf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_689672.9

ORF Insert Sequence:

ATGAGTGTGC TGAATATGAG CTCTCAGGAT AAGACAGACA CAGATCTTAG ACAGGAGGAG 
GAACACAGCA GCTCTGAGGA CAACAAACAG GAGGGGGTTA TTTGTCTGGG CAACAATGAA 
GTGAAGAGTC GTGCAGTAGT GAAGTATTCG GCTGCTCCTC CTCCCTCCTC CTATGCATTA 
CTACAAGAGA AGACCGACCT CAAGCTGCCA CCAGCAAACT GGCTGCGCGA AAATGCTCAG 
CTGGGACCCG CAGGGACCAC CATACTCGGG TCCAGCCGGA AAAGCAAACC CTTCTCCAGT 
TTTGGGATGG CGTTTGACTT CATTGACTGT ATAGGTGATG ATGTCGATGT TGTGTCTGAT 
TCAGAGAACA TCAAGAAGCT TCTGAAGATC CCGTACAGTA AATCTCATGT CAGCATGGCT 
GTTCATAGAG TTGGCAGCAC TTTACTGTTG GACGAACTGG ACATTCAGGA GCTCTTCATG 
AGGTCATCAC AGACTAATGA CTGGACGTGG CTGAAGGAGT TTTACCAGCG TCTTATAGAC 
CAAAAGTGGC AGAGAAAGAA GAAAAGTAAA GAGCACTGGA ACGAGAAGGC CATCCTCTCC 
AAATTCCTCT ATTACAGTAT AAACAGTGAT GGGACTGCAG TGTCTGTGCC GTCAGATACA 
GAGCAAACAG GAGACGAGGG GTGTGGGGCA GGAACTGATG GACCCTCTTG GCCTGCGACT 
TTCAGCAGCT CCACCACTGA TTCAGAAGAG TCTGCACTGC CCAAAGAGGA GCAGGTGGAC 
ACATATGCTT TAGGTCACGT GTCGTCGGTT CCCAAAGAAC AGAATCTACC CACGCTGTTT 
AATGAAGGAG AAAACAGTCA GGGTTTGAGA AATGACTTTG TCCGAAATAT TTTATGGACA 
TTTGAGGACA TCCACATGCT TGTGGGATCA AATATGCCAA TTTTCGGAGG TGGACGATAC 
CCAGCAGTCA GTTTACGTCT CAGGGACAAC AACAAACCAA TCAACGTTCT CACAGGAATT 
GACTACTGGC TGGATAATTT GATGTGTAAC GTACCAGAGC TGGTCATGTG CTTTCACGTC 
AATGGCATTG TTCAGAAATA TGAAATGATA AAAACAGAGG ACATCCCCAA TTTAGAGAAC 
TCCACATTCT CCACGCGGGT TGTGAAAGAC ATTGCTCAAA ATATCCTGTC GTTCCTGAAA 
TCAAACTGTA CCAAAGAAGG ACACACGTAC TGGCTCTTCA AGGCCAGCGG GAGTGACATT 
GTGAAGCTGT ACGACCTCAC TACACTGTGT GAAGAAGCAG CAGAGGAGAA ATGCCAGAAT 
CCCTTCACAC TTCCAGTAGC CGTGCTTCTC TACAAAGTGG CCAGCAACAT GATGCTAAAG 
AAGAGCCAAA ACCGGAAGAT CTATGGGACA ATCAGAACTC TACTGCTCAA CTGTATTAAA 
CTGTTGGATG AGGACAGACA TCCACAGATC ATCGCTTCGG CTCACTACAT GCTGGCAGAA 
CTGTTTCAGC TGGATGATGT GAGTGAAGAC GAAGGCAATG GGGAGTCGCT GAGAGGAGGA 
GGATCTGAAG ACAGCTACAG TGATGAAGAT GAGGAGGATG AGGAGGAAGA TGAAGACGAG 
AGATGCTCCT TCAGCACTGC CTCTGAACCG CAGCACGACT CCAAAGCTGT GGCCGTCATC 
AAGTCTGTCG GAGAGCTTTC GGTTCCAGAG AAATACAAGA GCACGCATCA GATCAGACCA 
AACTTTGCAT ATCCTGTTTC ACAAGACAAG GAGGAGAAGT GCCGACTGGT TCTCAGCTAC 
GTTTTAAAAG GTCTGAAAGC AGTGGACAGC AGCATAAAGA AAGAGAGCGA CCTTCCTGCA 
GCCGATCCCA GCACACCCAT CCCACTAAAA TATGAGGAAA GAAGTCCAAA TGGAGCTAGA 
GAGGTGGAGC AGGAGATTGC TTTACTGCTG GATAGAGTTG GCCCATGTAA CGAAGAGTTG 
AAGGTGCCGA CACGCTCCGG GATGTTACCA GGCACATGGC AGCATCGCAT GAAACTCCAG 
CTGTTCCTGA AAGCATCGAA AGCGTATTAC GTTTTGTCAG ATGCTGCGAC CAACCTACTG 
AAGTACGGCC GGGCACTGCG CTACATCAAA CTGGCTCTGC AGTGCCACGA TGCATACTGC 
TCCATTAGTG GCGGTCTCCA CGCACAGGTG CTTCTTTTCC ACTGCCAGTG TCTGTCTCTG 
TGTGGAGATG TTCAGCTCAT GTTGGCCCAG AATGCCTCAA ACAGAGCTGC GTATCTGGAG 
GAGTACAGCT ATCAGACCAA AGATGACCAG GAGATCCTCC ACTGCCTGCA CCGCGAGAGC 
TCCTGCCAAG CCTTCAGCAT GGCCACTGAT CTGGCCCTGG ATCCTGAGTA CCAGCTTATC 
GTCAGCTGCA AGTGTTATGA AGCCGCTCAT GATCTTTTGA CGTCAGGAGT CCTGAAAGAC 
CAGGCACAGG AGCAGCTGGG TCAGGTGCTG AAACGTCTCG GGAACATTCG AAACGAGATG 
GGCGTTTTCT ACATGAACCA GGCTGCAGCA ATGCAGGCAG AGAAAGAAGG AGTGCGCAGG 
AGTGTGTGTG TGGCCGAGCA GGAGCTGTGG AAGAAGAGCT TCTCGTGTTT TGAGAAGGGC 
ATGCAGTATT TTAGTGCTAT TTCAGACTCG GTGAACACGG CCCTGCTGCT GTGTAACATC 
GGCCGTCTCA TGCGCATCTG TGCTCAGGCT CACTGCGTCA TTACGTCAGA GCAGAGCAGA 
GGAGAGTTTT CACCTGAAGA GACTCTCTAT TACAACAAGG CCATAGATTA TTATCAGAGA 
GCGCTGAAGG CTCTGGGCTG CAGAGAGAGT CACTCCGCCG TCTGGGACTC GGTCAACTGG 
GAACTGTCCA CAACCTATTT CACACTGGCC ACACTCCTGC AGGATTACGC CCCACTGTCC 
CGCAAAGCAC AGGAGCAGAT CGAGAGGGAA GTGACCGAGA CCATGATGAA GTCTCTGAGA 
TACTGTGACC TGCAGACTGA ATCCGCTCGC CAGCCGCTCT ACCAGTACAG AGCAGCCACA 
ATCCACCACA GACTGGCTTC AATGTACCAC AGCTGCTACC GCAATCAGGT TGGAGATGAG 
AGTGATCGTA AGCAGCACAA AGCTCTGGCA GAGCAGCACT ACAGTAAAGC CGTGCGCCTC 
TTTGTGAATC TGAGTGATGC TCCGTGTGAA CTGCTCCGCA CACTTCTGGA GAGAGTGGCC 
TTCGCTGAGT TCACCATGGC AGGTCAGAAC AGCAGTGCTG TGAAACTGAA GACTCTGACA 
GGAGCTTTGG AGATCATGTG TGAAACACGG CATGCGTTCA GACTCATTCA TAAAGAGCTG 
CTGGAGCAAC AAGAAAATAT AACGAAGGCT GAAGAGAAAC CTGAAGAAGT CCCTGAGGGT 
TCAGAGAGTT CCTCTTCCTC TGAGCTGAAC ACAGAAGAGG TGCTGAAGCT AATTGGCATC 
TTCGAGCCCA GTTTCTCCTT CCTGTTACTG CAAGTGATTA AACTCAGCTC TGCAAGCAAA 
CGAAAACTCA GTGGTAAAGA AGAGGAGCAG GTGAAGATCT ACAAGAGCGT CTATTCTCGT 
CTCTTACGGG CTGAAAAGTC TGATCCACTC ATTCAGCGGG TTTCGCTTTT CCTGGAGCTC 
CTGGATCAGC TGAATCCACC ATCTGGAAGT GAAGAACCAA GATCATCATC ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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edrf1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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