thsd7ba cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	thsd7ba
Entrez Gene ID	566298
Full Name	thrombospondin, type I, domain containing 7Ba
Synonyms	si:dkey-118m15.1
General protein information	Preferred Names thrombospondin, type I, domain containing 7Ba Names thrombospondin type-1 domain-containing protein 7B thrombospondin, type I, domain containing 7B thsd7b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_689568.8	XP_694660.6	thrombospondin type-1 domain-containing protein 7B isoform X1
XM_009304519.2	XP_009302794.1	thrombospondin type-1 domain-containing protein 7B isoform X2
XM_017357762.1	XP_017213251.1	thrombospondin type-1 domain-containing protein 7B isoform X3
XM_689568.9	XP_694660.6	thrombospondin type-1 domain-containing protein 7B isoform X1
XM_009304519.3	XP_009302794.1	thrombospondin type-1 domain-containing protein 7B isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	THSD7B	XP_515814.4
Gallus gallus (chicken)	THSD7B	XP_422142.4
Danio rerio (zebrafish)	LOC566298	XP_694660.6
Xenopus tropicalis (tropical clawed frog)	thsd7b	XP_002933997.2
Homo sapiens (human)	THSD7B	NP_001073896.1
Canis lupus familiaris (dog)	THSD7B	XP_005632014.1
Bos taurus (cattle)	THSD7B	XP_003585799.2
Mus musculus (house mouse)	Thsd7b	NP_766073.2
Rattus norvegicus (Norway rat)	Thsd7b	NP_001178598.1

Gene Ontology

The following thsd7ba gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the thsd7ba cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa60305	XM_017357762.1 Latest version!	Danio rerio thrombospondin, type I, domain containing 7Ba (thsd7ba), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa14940	XM_689568.9 Latest version!	Danio rerio thrombospondin, type I, domain containing 7Ba (thsd7ba), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa44838	XM_009304519.3 Latest version!	Danio rerio thrombospondin, type I, domain containing 7Ba (thsd7ba), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
ODa14940	XM_689568.8	Danio rerio thrombospondin, type I, domain containing 7Ba (thsd7ba), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa44838	XM_009304519.2	Danio rerio thrombospondin, type I, domain containing 7Ba (thsd7ba), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

thsd7ba ( XM_017357762.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213251.1 Danio rerio thrombospondin, type I, domain containing 7Ba (thsd7ba), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa60305
Clone ID Related Accession (Same CDS sequence)	XM_017357762.1
Accession Version	XM_017357762.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	thrombospondin type-1 domain-containing protein 7B isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAATGG GCTGGACTAT GATTCTAAAA GATGACTCTG GTGTAACGGG CTGGACATGG 
CGGGTTGGGA ACTACCTGCT GCTCTCAATG TCTCTCATCG GAATGCATGT GAAGTCAGAC 
CTGGAATTTC CAAAGGACAG GCACTATACA TGGAAAACGG GTCATTGGGG CAGATGCATA 
GGGGAAGAAT GTGGTACCGG CGGGGTACAA TCCAGGACAG TGTGGTGCAT CCACACAGAA 
GGCTGGACCA CACACCACTC CAACTGTCGG CATTCAGACA AACCCGAGAG CCAGAGGCCT 
TGCTTTAAAG TATGTGAGTG GCACCAGGAC CTCTTTGAAT GGGAAGTGTC AGAATGGGGC 
CCTTGTGTTC TAGCCCCCCT CCTGTCCAAC GAGCTCAAAC TGCGGACCGC ACAGTGCATT 
ACAGCCCAAC ACGGCATCCA GAGACGGAAG GTCCATTGTG TGCGCACTTC CAACCGCACA 
TCGGTTACAG AAAGGATATG CGATTTCTTT TCACCACGAC CAGCTTTCGA GCAGGCCTGC 
TTGATCCCCT GCCCTCATGA CTGCATTGTG TCCGATTTCT CCTCTTGGTC TGGCTGCAGC 
AGAACATGCG GTGCGGGTCT TCAACATCGA ACCCGACATG TCCTAGCAGC GCCAATGTAC 
GGAGGCGCCA ACTGCCCCAA CCTCACCCAA ACAAGGACTT GCTCAAACCT TTTCCCCTGT 
CCTCTTGGGG AAAGCGAGCA TCACTACAGC CTTAAAGTCG GCCTCTGGAG CGAATGCAGG 
CAGCCACAAC ACAAAGGGCT TTGGATGAGC GGAAGGACCG TGCTAGACTT CAGCACAGGA 
GATACCGAAA GAAACACAGT GAAACGACAT ATTCAGAGCT CCCAGCACCA CCATCACCAC 
CTCCACCAAC ACCACCACCA GCACAGTCTC AAGGGTTTAG ATGTGGAGAT CGGCTATCAA 
ACACGGCAAG TTCGTTGCAT GCGCAGCGAT GGAAAAAACG CCATGCTGAG CCTTTGTACC 
CTTGACAACG GCCCTGGCAC TTTCCAGTCT TGTGTTATGC CCAGAGACTG CGAGACCTCC 
GATTGGTCCG TTTGGAGTCC ATGTTCAAAG ACATGCAGAG CATCAGATAT GTCGCCTGGT 
TTTCGGGTCA GGACTCGAAG CATAACGCAC ACTCCCATTG GTCAAGGCAA AGGATGCCCT 
GCTCTTGAAG AAAAGGAGGC CTGCAACATC ATTGGAGATC TGCTTCCAAA CTGCCCAAGG 
TACGTGTGGA AGAGCACCGA TTGGGGGGAC TGTCAAGTGG CCCCTCTTCT TAGCCAGCAG 
GACAGAAGGC TTGACAATGT TAGCGTTCTC TGTGGTGGAG GGATTCAGAC CAGGCAGCTG 
TACTGCGTCC AGGTCCCAGA CAATTCCACC CCTCATCATA GAAAAGAGGT GTCTAGGCCT 
GTAAATGTGC ATCTGTGCAC AGGAGATCCG CCTTCTGCAG TGCAGCCCTG CTCCATTCCA 
TGCCCACAGC TGTGCCTGCT CTCATCCTGG TCCTCCTGGG GAGCCTGTAT CCACGACAAC 
TGCATGGAGA CGCATGGACG AAAAGGCTTC AAGCAGAGGA GGAGGGATGT GCTGGAGGAA 
GGATCTGGAA CCGAGAACTG TGCTCATCTC CTGGAGTCAA TGCCCTGTGA GGATCCACTG 
TGCTTCCGGT GGCAGGTGCA GATGGAGAGT GCTTGTGTGC CCAGCAATGG CACCTGCGGG 
AATGGAACCA GGACACAAAC TGTGGCTTGC ATCAATTCAC AAGGGCAGCT TGTTCCAGAG 
GAGCTTTGTG AAGAAGAGTC TCCCCCTGCT GAGGTGTCCT GTGTGATGGC CTGTTCTGGT 
GACTGTGTGC TCAGCTCCTG GTCTCACTGG TCCTCCTGCT CTCACAGCTG CTCCAGCAAA 
AACTCTGAAG GCCGTCAGAG CCGCATACGC ACCATATTAG GCTTGCCAGG AGAAGATAAG 
ACGTGTCCAG CTGCTACTGC TCTGGAGGAG TGGAGAACTT GTAATGATCA TCCATGCATC 
ACCTTCTTCT GGGAAGCTTC GCCGTGGGGG CCTTGCATTG AAGACTCCTC TATGAATGTA 
AATGGCAGCG GCTACTGGAA CGGAACGGCC ACCTGTGCAG TCGGGCTGCA GATCCGTAAA 
GTGGCCTGCA TGAAAATGGG CGTTGGGCCG GTTATTCCCA AAAGGTGTCC AGAATCTGCC 
CGTCCAGACA CCATTCAGCC CTGTTTACTG CCCTGTGAGA AAGATTGCAT TGTGACTCCT 
TTCAGCGAAT GGACAGCCTG CCCGACCACA TGCCTGCCAG TGAACAGCAG CGTGGCCTCA 
CAGTCACGAT ACAGGATCAT CATCCAGCGG GCGGCCAATG GAGGTCAAGA GTGTCCCGAT 
ACTTTATTTG AAGAGAGGGA ATGTGAAGCT CTTCTGCTTT GTCCCACTTA CAGATGGAAG 
ACACACAGAT GGCATCCGTG TACTCTGGTC CCAGATTCAG TCCAGCAGGG AATGACCGGA 
GCGAGTGAAT CGTGTGGTAA AGGATTAGAG ATGAGAGGGC ACTGCGGGGA ACCAAGAACA 
AGTGTGAGAA AGAAAAGGGA GAAAGTAGAG AACGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213251.1
CDS	491..3109
Translation	MSMGWTMILKDDSGVTGWTWRVGNYLLLSMSLIGMHVKSDLEFPKDRHYTWKTGHWGRCIGEECGTGGVQSRTVWCIHTEGWTTHHSNCRHSDKPESQRPCFKVCEWHQDLFEWEVSEWGPCVLAPLLSNELKLRTAQCITAQHGIQRRKVHCVRTSNRTSVTERICDFFSPRPAFEQACLIPCPHDCIVSDFSSWSGCSRTCGAGLQHRTRHVLAAPMYGGANCPNLTQTRTCSNLFPCPLGESEHHYSLKVGLWSECRQPQHKGLWMSGRTVLDFSTGDTERNTVKRHIQSSQHHHHHLHQHHHQHSLKGLDVEIGYQTRQVRCMRSDGKNAMLSLCTLDNGPGTFQSCVMPRDCETSDWSVWSPCSKTCRASDMSPGFRVRTRSITHTPIGQGKGCPALEEKEACNIIGDLLPNCPRYVWKSTDWGDCQVAPLLSQQDRRLDNVSVLCGGGIQTRQLYCVQVPDNSTPHHRKEVSRPVNVHLCTGDPPSAVQPCSIPCPQLCLLSSWSSWGACIHDNCMETHGRKGFKQRRRDVLEEGSGTENCAHLLESMPCEDPLCFRWQVQMESACVPSNGTCGNGTRTQTVACINSQGQLVPEELCEEESPPAEVSCVMACSGDCVLSSWSHWSSCSHSCSSKNSEGRQSRIRTILGLPGEDKTCPAATALEEWRTCNDHPCITFFWEASPWGPCIEDSSMNVNGSGYWNGTATCAVGLQIRKVACMKMGVGPVIPKRCPESARPDTIQPCLLPCEKDCIVTPFSEWTACPTTCLPVNSSVASQSRYRIIIQRAANGGQECPDTLFEERECEALLLCPTYRWKTHRWHPCTLVPDSVQQGMTGASESCGKGLEMRGHCGEPRTSVRKKREKVENE

Target ORF information:

RefSeq Version	XM_017357762.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio thrombospondin, type I, domain containing 7Ba (thsd7ba), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357762.1

ORF Insert Sequence:

ATGTCAATGG GCTGGACTAT GATTCTAAAA GATGACTCTG GTGTAACGGG CTGGACATGG 
CGGGTTGGGA ACTACCTGCT GCTCTCAATG TCTCTCATCG GAATGCATGT GAAGTCAGAC 
CTGGAATTTC CAAAGGACAG GCACTATACA TGGAAAACGG GTCATTGGGG CAGATGCATA 
GGGGAAGAAT GTGGTACCGG CGGGGTACAA TCCAGGACAG TGTGGTGCAT CCACACAGAA 
GGCTGGACCA CACACCACTC CAACTGTCGG CATTCAGACA AACCCGAGAG CCAGAGGCCT 
TGCTTTAAAG TATGTGAGTG GCACCAGGAC CTCTTTGAAT GGGAAGTGTC AGAATGGGGC 
CCTTGTGTTC TAGCCCCCCT CCTGTCCAAC GAGCTCAAAC TGCGGACCGC ACAGTGCATT 
ACAGCCCAAC ACGGCATCCA GAGACGGAAG GTCCATTGTG TGCGCACTTC CAACCGCACA 
TCGGTTACAG AAAGGATATG CGATTTCTTT TCACCACGAC CAGCTTTCGA GCAGGCCTGC 
TTGATCCCCT GCCCTCATGA CTGCATTGTG TCCGATTTCT CCTCTTGGTC TGGCTGCAGC 
AGAACATGCG GTGCGGGTCT TCAACATCGA ACCCGACATG TCCTAGCAGC GCCAATGTAC 
GGAGGCGCCA ACTGCCCCAA CCTCACCCAA ACAAGGACTT GCTCAAACCT TTTCCCCTGT 
CCTCTTGGGG AAAGCGAGCA TCACTACAGC CTTAAAGTCG GCCTCTGGAG CGAATGCAGG 
CAGCCACAAC ACAAAGGGCT TTGGATGAGC GGAAGGACCG TGCTAGACTT CAGCACAGGA 
GATACCGAAA GAAACACAGT GAAACGACAT ATTCAGAGCT CCCAGCACCA CCATCACCAC 
CTCCACCAAC ACCACCACCA GCACAGTCTC AAGGGTTTAG ATGTGGAGAT CGGCTATCAA 
ACACGGCAAG TTCGTTGCAT GCGCAGCGAT GGAAAAAACG CCATGCTGAG CCTTTGTACC 
CTTGACAACG GCCCTGGCAC TTTCCAGTCT TGTGTTATGC CCAGAGACTG CGAGACCTCC 
GATTGGTCCG TTTGGAGTCC ATGTTCAAAG ACATGCAGAG CATCAGATAT GTCGCCTGGT 
TTTCGGGTCA GGACTCGAAG CATAACGCAC ACTCCCATTG GTCAAGGCAA AGGATGCCCT 
GCTCTTGAAG AAAAGGAGGC CTGCAACATC ATTGGAGATC TGCTTCCAAA CTGCCCAAGG 
TACGTGTGGA AGAGCACCGA TTGGGGGGAC TGTCAAGTGG CCCCTCTTCT TAGCCAGCAG 
GACAGAAGGC TTGACAATGT TAGCGTTCTC TGTGGTGGAG GGATTCAGAC CAGGCAGCTG 
TACTGCGTCC AGGTCCCAGA CAATTCCACC CCTCATCATA GAAAAGAGGT GTCTAGGCCT 
GTAAATGTGC ATCTGTGCAC AGGAGATCCG CCTTCTGCAG TGCAGCCCTG CTCCATTCCA 
TGCCCACAGC TGTGCCTGCT CTCATCCTGG TCCTCCTGGG GAGCCTGTAT CCACGACAAC 
TGCATGGAGA CGCATGGACG AAAAGGCTTC AAGCAGAGGA GGAGGGATGT GCTGGAGGAA 
GGATCTGGAA CCGAGAACTG TGCTCATCTC CTGGAGTCAA TGCCCTGTGA GGATCCACTG 
TGCTTCCGGT GGCAGGTGCA GATGGAGAGT GCTTGTGTGC CCAGCAATGG CACCTGCGGG 
AATGGAACCA GGACACAAAC TGTGGCTTGC ATCAATTCAC AAGGGCAGCT TGTTCCAGAG 
GAGCTTTGTG AAGAAGAGTC TCCCCCTGCT GAGGTGTCCT GTGTGATGGC CTGTTCTGGT 
GACTGTGTGC TCAGCTCCTG GTCTCACTGG TCCTCCTGCT CTCACAGCTG CTCCAGCAAA 
AACTCTGAAG GCCGTCAGAG CCGCATACGC ACCATATTAG GCTTGCCAGG AGAAGATAAG 
ACGTGTCCAG CTGCTACTGC TCTGGAGGAG TGGAGAACTT GTAATGATCA TCCATGCATC 
ACCTTCTTCT GGGAAGCTTC GCCGTGGGGG CCTTGCATTG AAGACTCCTC TATGAATGTA 
AATGGCAGCG GCTACTGGAA CGGAACGGCC ACCTGTGCAG TCGGGCTGCA GATCCGTAAA 
GTGGCCTGCA TGAAAATGGG CGTTGGGCCG GTTATTCCCA AAAGGTGTCC AGAATCTGCC 
CGTCCAGACA CCATTCAGCC CTGTTTACTG CCCTGTGAGA AAGATTGCAT TGTGACTCCT 
TTCAGCGAAT GGACAGCCTG CCCGACCACA TGCCTGCCAG TGAACAGCAG CGTGGCCTCA 
CAGTCACGAT ACAGGATCAT CATCCAGCGG GCGGCCAATG GAGGTCAAGA GTGTCCCGAT 
ACTTTATTTG AAGAGAGGGA ATGTGAAGCT CTTCTGCTTT GTCCCACTTA CAGATGGAAG 
ACACACAGAT GGCATCCGTG TACTCTGGTC CCAGATTCAG TCCAGCAGGG AATGACCGGA 
GCGAGTGAAT CGTGTGGTAA AGGATTAGAG ATGAGAGGGC ACTGCGGGGA ACCAAGAACA 
AGTGTGAGAA AGAAAAGGGA GAAAGTAGAG AACGAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

thsd7ba ( XM_689568.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_694660.6 Danio rerio thrombospondin, type I, domain containing 7Ba (thsd7ba), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa14940
Clone ID Related Accession (Same CDS sequence)	XM_689568.8 , XM_689568.9
Accession Version	XM_689568.8	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	thrombospondin type-1 domain-containing protein 7B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_689568.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAATGG GCTGGACTAT GATTCTAAAA GATGACTCTG GTGTAACGGG CTGGACATGG 
CGGGTTGGGA ACTACCTGCT GCTCTCAATG TCTCTCATCG GAATGCATGT GAAGTCAGAC 
CTGGAATTTC CAAAGGACAG GCACTATACA TGGAAAACGG GTCATTGGGG CAGATGCATA 
GGGGAAGAAT GTGGTACCGG CGGGGTACAA TCCAGGACAG TGTGGTGCAT CCACACAGAA 
GGCTGGACCA CACACCACTC CAACTGTCGG CATTCAGACA AACCCGAGAG CCAGAGGCCT 
TGCTTTAAAG TATGTGAGTG GCACCAGGAC CTCTTTGAAT GGGAAGTGTC AGAATGGGGC 
CCTTGTGTTC TAGCCCCCCT CCTGTCCAAC GAGCTCAAAC TGCGGACCGC ACAGTGCATT 
ACAGCCCAAC ACGGCATCCA GAGACGGAAG GTCCATTGTG TGCGCACTTC CAACCGCACA 
TCGGTTACAG AAAGGATATG CGATTTCTTT TCACCACGAC CAGCTTTCGA GCAGGCCTGC 
TTGATCCCCT GCCCTCATGA CTGCATTGTG TCCGATTTCT CCTCTTGGTC TGGCTGCAGC 
AGAACATGCG GTGCGGGTCT TCAACATCGA ACCCGACATG TCCTAGCAGC GCCAATGTAC 
GGAGGCGCCA ACTGCCCCAA CCTCACCCAA ACAAGGACTT GCTCAAACCT TTTCCCCTGT 
CCTCTTGGGG AAAGCGAGCA TCACTACAGC CTTAAAGTCG GCCTCTGGAG CGAATGCAGG 
CAGCCACAAC ACAAAGGGCT TTGGATGAGC GGAAGGACCG TGCTAGACTT CAGCACAGGA 
GATACCGAAA GAAACACAGT GAAACGACAT ATTCAGAGCT CCCAGCACCA CCATCACCAC 
CTCCACCAAC ACCACCACCA GCACAGTCTC AAGGGTTTAG ATGTGGAGAT CGGCTATCAA 
ACACGGCAAG TTCGTTGCAT GCGCAGCGAT GGAAAAAACG CCATGCTGAG CCTTTGTACC 
CTTGACAACG GCCCTGGCAC TTTCCAGTCT TGTGTTATGC CCAGAGACTG CGAGACCTCC 
GATTGGTCCG TTTGGAGTCC ATGTTCAAAG ACATGCAGAG CATCAGATAT GTCGCCTGGT 
TTTCGGGTCA GGACTCGAAG CATAACGCAC ACTCCCATTG GTCAAGGCAA AGGATGCCCT 
GCTCTTGAAG AAAAGGAGGC CTGCAACATC ATTGGAGATC TGCTTCCAAA CTGCCCAAGG 
TACGTGTGGA AGAGCACCGA TTGGGGGGAC TGTCAAGTGG CCCCTCTTCT TAGCCAGCAG 
GACAGAAGGC TTGACAATGT TAGCGTTCTC TGTGGTGGAG GGATTCAGAC CAGGCAGCTG 
TACTGCGTCC AGGTCCCAGA CAATTCCACC CCTCATCATA GAAAAGAGGT GTCTAGGCCT 
GTAAATGTGC ATCTGTGCAC AGGAGATCCG CCTTCTGCAG TGCAGCCCTG CTCCATTCCA 
TGCCCACAGC TGTGCCTGCT CTCATCCTGG TCCTCCTGGG GAGCCTGTAT CCACGACAAC 
TGCATGGAGA CGCATGGACG AAAAGGCTTC AAGCAGAGGA GGAGGGATGT GCTGGAGGAA 
GGATCTGGAA CCGAGAACTG TGCTCATCTC CTGGAGTCAA TGCCCTGTGA GGATCCACTG 
TGCTTCCGGT GGCAGGTGCA GATGGAGAGT GCTTGTGTGC CCAGCAATGG CACCTGCGGG 
AATGGAACCA GGACACAAAC TGTGGCTTGC ATCAATTCAC AAGGGCAGCT TGTTCCAGAG 
GAGCTTTGTG AAGAAGAGTC TCCCCCTGCT GAGGTGTCCT GTGTGATGGC CTGTTCTGGT 
GACTGTGTGC TCAGCTCCTG GTCTCACTGG TCCTCCTGCT CTCACAGCTG CTCCAGCAAA 
AACTCTGAAG GCCGTCAGAG CCGCATACGC ACCATATTAG GCTTGCCAGG AGAAGATAAG 
ACGTGTCCAG CTGCTACTGC TCTGGAGGAG TGGAGAACTT GTAATGATCA TCCATGCATC 
ACCTTCTTCT GGGAAGCTTC GCCGTGGGGG CCTTGCATTG AAGACTCCTC TATGAATGTA 
AATGGCAGCG GCTACTGGAA CGGAACGGCC ACCTGTGCAG TCGGGCTGCA GATCCGTAAA 
GTGGCCTGCA TGAAAATGGG CGTTGGGCCG GTTATTCCCA AAAGGTGTCC AGAATCTGCC 
CGTCCAGACA CCATTCAGCC CTGTTTACTG CCCTGTGAGA AAGATTGCAT TGTGACTCCT 
TTCAGCGAAT GGACAGCCTG CCCGACCACA TGCCTGCCAG TGAACAGCAG CGTGGCCTCA 
CAGTCACGAT ACAGGATCAT CATCCAGCGG GCGGCCAATG GAGGTCAAGA GTGTCCCGAT 
ACTTTATTTG AAGAGAGGGA ATGTGAAGCT CTTCTGCTTT GTCCCACTTA CAGATGGAAG 
ACACACAGAT GGCATCCGTG TACTCTGGTC CCAGATTCAG TCCAGCAGGG AATGACCGGA 
GCGAGTGAAT CGTGTGGTAA AGGATTAGAG ATGAGAGGCG TGAGCTGTGT GAATGAGTAT 
GATGAGATGG TGGCCGTGTC CGAGTGTCTC CAGTGGGCCG GGCCGATGCC GTCTCGCTTC 
AGGAGCTGTT GGGTGGCATG TAAAGATGAC TGTGCCTTTA GTTCCTGGTC GAAATTTTCT 
GAATGCGCTG GATGTGGGAG CAGACGGACA CGCAAACGCA CAATTACAGG TCGTAGCCGA 
AAACTAGACC GATGTCAGCG GGAAGACTTG TTCCCTCTTG TGGAGTCCGA ACCCTGTCCG 
TGTGAGGAGT TTGTTTCTCA GCCATACGGT AATTGGTCGT CTTGTATCCT GTCGGAGACC 
CCAGCACAGG GTTGGAGGGG TCGTCGAGAG GCACGCGACT GTGGTCAGGG ATTCCGCTAT 
CGAGCAATGT CCTGTCTAGA CCACGAATCC CACCTGGTCA GCCCAGAACT CTGCAGCGAA 
TCAGGTCTGG TGGAAGAGGT CTGCTACATA CCCTGCCCTC TGGACTGCAA GCTGAGCGAC 
TGGTCACCGT GGTCAGCCTG CAGCGCCTCC TGTGGCAGTG GACTGAAAAT CAGGTCCAAA 
TGGCTACGAG AGAAGCCTTT CAACGGGGGG AGGCCGTGTC CAAAACTAGA CCTCAAGAAT 
CAGGCTCAGG TGTATGAGGC AGTTCCCTGT CGAAGTGAGT GTGATCAGTA CGAGTGGCTA 
ACAGACCCAT GGTCAGTCTG TACCATCAAC ACAGTGGATG AGCTGCCAGC CTGTGGAGAG 
GGAGTCCAGA GCCGCAAGAT CTGGTGTGTG AGTCGTGGCA GTGAGGACCT GGTGGCCAGT 
GTGAATGAGA CCCTGTGTGA CCAGGATGAA ATTCCTCCTC AAGCGCAGAC CTGCGTCCTG 
CCGTGTCCTG ACGACTGCGT GATGTCCCAG TGGAGTCAGT GGAGCACCTG TGCCATACCG 
TGTGACGAAA ACAGCTCGAG GACTAGGACA AGGCACGTGC TGCGCCCCCC GGTGGCTGAC 
AGAGTGTGTC CAGAAAATAA TCAGACGGAG CCCTGCATCC TCAACAGCAC CTGCTTCAAT 
TACCACTACA ATGTTTCCAG CTGGAGCACC TGTCAGCTGA GCGAACATGC CATCTGCGGC 
CAGGGCACGA GGACACGACT CTTAGATTGC ATCCGCAGCG ATGGCAAAGT GGTGGATTTG 
AGCGTGTGCG AGGAGTTGGG CCTGCTTCAG CTGTGGAGAC TGACCATGAA ATGTGTGGTG 
GACTGTCCCT TTAACTGCAT CCTGTCTGAC TGGACGTCCT GGACGGAGTG TTCACACACC 
TGTGGAAGTA AAGGTGTTAA AACTCGCTTT CGCACTGTGC TCCAGCCGGC TCATGAGGAG 
GGACGTCCCT GCGCCTCCCA GCTGTCCCAA ACCAAGCCTT GTCCCATCAG CCCCTGCTAC 
ACGTGGCTAC TGAGCGACTG GTCTCCCTGC AGAGTCCAGG GAGCTGAATG TGGAGAGGGC 
ACGAGGGAGA GGAACCTGAC GTGTGTACAG CACTGGTCAG ACTGGCCGGA CAGTCCAAAC 
CCTTCTGAAG CCCTCGAGGA CGATAAGTGT GGAGACCGAC TCCGAAAGCA GAGTGAGCAG 
GAGCTGCAGC AGCCGTGCTT TGTTCCCTGC CCAGGAGACT GTCACCTGAC GGAGTGGTCA 
TTGTGGAGCT CCTGTCAGCT CACGTGTTTG GAGGGTCGCA GTTTCGAGAC AGTGGGGCGT 
CAGGCACGTT CACAAGCTGT TGTCATTCAG GTGCTGGACA ATCAGGACAG CTGCCCTCAG 
CAGGTTTTCG AGACCAGGCC GTGCAAAGGA GGGAAATGTC ACAGTTACGA GTGGAGGACC 
AGTGCCTGGA ATGACAACGA GAGATCAGTG TGGTGCCAGC GGTCTGACGG GGTCAATGTG 
ACAGGGGGCT GCTTTCCACA GAACCGGCCA ACCACTGTGC GCCACTGTCA CCCTCCCTGC 
ACTAAACCCT TCTCCCACTG CAAGCAGAGT GGTGTGTGTG GCTGTGAGAG AGGCTACACT 
GAAGTGATGA CCACACATGG CTTTTTGGAC TACTGCACCA AAACCCCAGG CACCGACAAC 
AAGAAGGCCG ATGTGAAGAC CAGCTCAGGA AGATTAAGAC CAGGCACGAC TCAAATCCAG 
GACTTCTTCG GCGAGTGGTC CCTGCAGCCT CTCGGCCCAG ATGGAAGAAT AAAGCTGTGG 
GTCTACGGGT TAACAGCTGG AGGATTCATT GTCATATTAT TCACAATTGC TGTGGCCTTT 
CTGTTTTGCA AACCATCCAA ACAGAGTAAA AGTGCATCTC CACCACAGAA GCCCTTGACT 
CTGGCCTATG ATGGCGATGT GGACATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_694660.6
CDS	491..5380
Translation	MSMGWTMILKDDSGVTGWTWRVGNYLLLSMSLIGMHVKSDLEFPKDRHYTWKTGHWGRCIGEECGTGGVQSRTVWCIHTEGWTTHHSNCRHSDKPESQRPCFKVCEWHQDLFEWEVSEWGPCVLAPLLSNELKLRTAQCITAQHGIQRRKVHCVRTSNRTSVTERICDFFSPRPAFEQACLIPCPHDCIVSDFSSWSGCSRTCGAGLQHRTRHVLAAPMYGGANCPNLTQTRTCSNLFPCPLGESEHHYSLKVGLWSECRQPQHKGLWMSGRTVLDFSTGDTERNTVKRHIQSSQHHHHHLHQHHHQHSLKGLDVEIGYQTRQVRCMRSDGKNAMLSLCTLDNGPGTFQSCVMPRDCETSDWSVWSPCSKTCRASDMSPGFRVRTRSITHTPIGQGKGCPALEEKEACNIIGDLLPNCPRYVWKSTDWGDCQVAPLLSQQDRRLDNVSVLCGGGIQTRQLYCVQVPDNSTPHHRKEVSRPVNVHLCTGDPPSAVQPCSIPCPQLCLLSSWSSWGACIHDNCMETHGRKGFKQRRRDVLEEGSGTENCAHLLESMPCEDPLCFRWQVQMESACVPSNGTCGNGTRTQTVACINSQGQLVPEELCEEESPPAEVSCVMACSGDCVLSSWSHWSSCSHSCSSKNSEGRQSRIRTILGLPGEDKTCPAATALEEWRTCNDHPCITFFWEASPWGPCIEDSSMNVNGSGYWNGTATCAVGLQIRKVACMKMGVGPVIPKRCPESARPDTIQPCLLPCEKDCIVTPFSEWTACPTTCLPVNSSVASQSRYRIIIQRAANGGQECPDTLFEERECEALLLCPTYRWKTHRWHPCTLVPDSVQQGMTGASESCGKGLEMRGVSCVNEYDEMVAVSECLQWAGPMPSRFRSCWVACKDDCAFSSWSKFSECAGCGSRRTRKRTITGRSRKLDRCQREDLFPLVESEPCPCEEFVSQPYGNWSSCILSETPAQGWRGRREARDCGQGFRYRAMSCLDHESHLVSPELCSESGLVEEVCYIPCPLDCKLSDWSPWSACSASCGSGLKIRSKWLREKPFNGGRPCPKLDLKNQAQVYEAVPCRSECDQYEWLTDPWSVCTINTVDELPACGEGVQSRKIWCVSRGSEDLVASVNETLCDQDEIPPQAQTCVLPCPDDCVMSQWSQWSTCAIPCDENSSRTRTRHVLRPPVADRVCPENNQTEPCILNSTCFNYHYNVSSWSTCQLSEHAICGQGTRTRLLDCIRSDGKVVDLSVCEELGLLQLWRLTMKCVVDCPFNCILSDWTSWTECSHTCGSKGVKTRFRTVLQPAHEEGRPCASQLSQTKPCPISPCYTWLLSDWSPCRVQGAECGEGTRERNLTCVQHWSDWPDSPNPSEALEDDKCGDRLRKQSEQELQQPCFVPCPGDCHLTEWSLWSSCQLTCLEGRSFETVGRQARSQAVVIQVLDNQDSCPQQVFETRPCKGGKCHSYEWRTSAWNDNERSVWCQRSDGVNVTGGCFPQNRPTTVRHCHPPCTKPFSHCKQSGVCGCERGYTEVMTTHGFLDYCTKTPGTDNKKADVKTSSGRLRPGTTQIQDFFGEWSLQPLGPDGRIKLWVYGLTAGGFIVILFTIAVAFLFCKPSKQSKSASPPQKPLTLAYDGDVDM

Target ORF information:

RefSeq Version	XM_689568.8
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio thrombospondin, type I, domain containing 7Ba (thsd7ba), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_689568.8

ORF Insert Sequence:

ATGTCAATGG GCTGGACTAT GATTCTAAAA GATGACTCTG GTGTAACGGG CTGGACATGG 
CGGGTTGGGA ACTACCTGCT GCTCTCAATG TCTCTCATCG GAATGCATGT GAAGTCAGAC 
CTGGAATTTC CAAAGGACAG GCACTATACA TGGAAAACGG GTCATTGGGG CAGATGCATA 
GGGGAAGAAT GTGGTACCGG CGGGGTACAA TCCAGGACAG TGTGGTGCAT CCACACAGAA 
GGCTGGACCA CACACCACTC CAACTGTCGG CATTCAGACA AACCCGAGAG CCAGAGGCCT 
TGCTTTAAAG TATGTGAGTG GCACCAGGAC CTCTTTGAAT GGGAAGTGTC AGAATGGGGC 
CCTTGTGTTC TAGCCCCCCT CCTGTCCAAC GAGCTCAAAC TGCGGACCGC ACAGTGCATT 
ACAGCCCAAC ACGGCATCCA GAGACGGAAG GTCCATTGTG TGCGCACTTC CAACCGCACA 
TCGGTTACAG AAAGGATATG CGATTTCTTT TCACCACGAC CAGCTTTCGA GCAGGCCTGC 
TTGATCCCCT GCCCTCATGA CTGCATTGTG TCCGATTTCT CCTCTTGGTC TGGCTGCAGC 
AGAACATGCG GTGCGGGTCT TCAACATCGA ACCCGACATG TCCTAGCAGC GCCAATGTAC 
GGAGGCGCCA ACTGCCCCAA CCTCACCCAA ACAAGGACTT GCTCAAACCT TTTCCCCTGT 
CCTCTTGGGG AAAGCGAGCA TCACTACAGC CTTAAAGTCG GCCTCTGGAG CGAATGCAGG 
CAGCCACAAC ACAAAGGGCT TTGGATGAGC GGAAGGACCG TGCTAGACTT CAGCACAGGA 
GATACCGAAA GAAACACAGT GAAACGACAT ATTCAGAGCT CCCAGCACCA CCATCACCAC 
CTCCACCAAC ACCACCACCA GCACAGTCTC AAGGGTTTAG ATGTGGAGAT CGGCTATCAA 
ACACGGCAAG TTCGTTGCAT GCGCAGCGAT GGAAAAAACG CCATGCTGAG CCTTTGTACC 
CTTGACAACG GCCCTGGCAC TTTCCAGTCT TGTGTTATGC CCAGAGACTG CGAGACCTCC 
GATTGGTCCG TTTGGAGTCC ATGTTCAAAG ACATGCAGAG CATCAGATAT GTCGCCTGGT 
TTTCGGGTCA GGACTCGAAG CATAACGCAC ACTCCCATTG GTCAAGGCAA AGGATGCCCT 
GCTCTTGAAG AAAAGGAGGC CTGCAACATC ATTGGAGATC TGCTTCCAAA CTGCCCAAGG 
TACGTGTGGA AGAGCACCGA TTGGGGGGAC TGTCAAGTGG CCCCTCTTCT TAGCCAGCAG 
GACAGAAGGC TTGACAATGT TAGCGTTCTC TGTGGTGGAG GGATTCAGAC CAGGCAGCTG 
TACTGCGTCC AGGTCCCAGA CAATTCCACC CCTCATCATA GAAAAGAGGT GTCTAGGCCT 
GTAAATGTGC ATCTGTGCAC AGGAGATCCG CCTTCTGCAG TGCAGCCCTG CTCCATTCCA 
TGCCCACAGC TGTGCCTGCT CTCATCCTGG TCCTCCTGGG GAGCCTGTAT CCACGACAAC 
TGCATGGAGA CGCATGGACG AAAAGGCTTC AAGCAGAGGA GGAGGGATGT GCTGGAGGAA 
GGATCTGGAA CCGAGAACTG TGCTCATCTC CTGGAGTCAA TGCCCTGTGA GGATCCACTG 
TGCTTCCGGT GGCAGGTGCA GATGGAGAGT GCTTGTGTGC CCAGCAATGG CACCTGCGGG 
AATGGAACCA GGACACAAAC TGTGGCTTGC ATCAATTCAC AAGGGCAGCT TGTTCCAGAG 
GAGCTTTGTG AAGAAGAGTC TCCCCCTGCT GAGGTGTCCT GTGTGATGGC CTGTTCTGGT 
GACTGTGTGC TCAGCTCCTG GTCTCACTGG TCCTCCTGCT CTCACAGCTG CTCCAGCAAA 
AACTCTGAAG GCCGTCAGAG CCGCATACGC ACCATATTAG GCTTGCCAGG AGAAGATAAG 
ACGTGTCCAG CTGCTACTGC TCTGGAGGAG TGGAGAACTT GTAATGATCA TCCATGCATC 
ACCTTCTTCT GGGAAGCTTC GCCGTGGGGG CCTTGCATTG AAGACTCCTC TATGAATGTA 
AATGGCAGCG GCTACTGGAA CGGAACGGCC ACCTGTGCAG TCGGGCTGCA GATCCGTAAA 
GTGGCCTGCA TGAAAATGGG CGTTGGGCCG GTTATTCCCA AAAGGTGTCC AGAATCTGCC 
CGTCCAGACA CCATTCAGCC CTGTTTACTG CCCTGTGAGA AAGATTGCAT TGTGACTCCT 
TTCAGCGAAT GGACAGCCTG CCCGACCACA TGCCTGCCAG TGAACAGCAG CGTGGCCTCA 
CAGTCACGAT ACAGGATCAT CATCCAGCGG GCGGCCAATG GAGGTCAAGA GTGTCCCGAT 
ACTTTATTTG AAGAGAGGGA ATGTGAAGCT CTTCTGCTTT GTCCCACTTA CAGATGGAAG 
ACACACAGAT GGCATCCGTG TACTCTGGTC CCAGATTCAG TCCAGCAGGG AATGACCGGA 
GCGAGTGAAT CGTGTGGTAA AGGATTAGAG ATGAGAGGCG TGAGCTGTGT GAATGAGTAT 
GATGAGATGG TGGCCGTGTC CGAGTGTCTC CAGTGGGCCG GGCCGATGCC GTCTCGCTTC 
AGGAGCTGTT GGGTGGCATG TAAAGATGAC TGTGCCTTTA GTTCCTGGTC GAAATTTTCT 
GAATGCGCTG GATGTGGGAG CAGACGGACA CGCAAACGCA CAATTACAGG TCGTAGCCGA 
AAACTAGACC GATGTCAGCG GGAAGACTTG TTCCCTCTTG TGGAGTCCGA ACCCTGTCCG 
TGTGAGGAGT TTGTTTCTCA GCCATACGGT AATTGGTCGT CTTGTATCCT GTCGGAGACC 
CCAGCACAGG GTTGGAGGGG TCGTCGAGAG GCACGCGACT GTGGTCAGGG ATTCCGCTAT 
CGAGCAATGT CCTGTCTAGA CCACGAATCC CACCTGGTCA GCCCAGAACT CTGCAGCGAA 
TCAGGTCTGG TGGAAGAGGT CTGCTACATA CCCTGCCCTC TGGACTGCAA GCTGAGCGAC 
TGGTCACCGT GGTCAGCCTG CAGCGCCTCC TGTGGCAGTG GACTGAAAAT CAGGTCCAAA 
TGGCTACGAG AGAAGCCTTT CAACGGGGGG AGGCCGTGTC CAAAACTAGA CCTCAAGAAT 
CAGGCTCAGG TGTATGAGGC AGTTCCCTGT CGAAGTGAGT GTGATCAGTA CGAGTGGCTA 
ACAGACCCAT GGTCAGTCTG TACCATCAAC ACAGTGGATG AGCTGCCAGC CTGTGGAGAG 
GGAGTCCAGA GCCGCAAGAT CTGGTGTGTG AGTCGTGGCA GTGAGGACCT GGTGGCCAGT 
GTGAATGAGA CCCTGTGTGA CCAGGATGAA ATTCCTCCTC AAGCGCAGAC CTGCGTCCTG 
CCGTGTCCTG ACGACTGCGT GATGTCCCAG TGGAGTCAGT GGAGCACCTG TGCCATACCG 
TGTGACGAAA ACAGCTCGAG GACTAGGACA AGGCACGTGC TGCGCCCCCC GGTGGCTGAC 
AGAGTGTGTC CAGAAAATAA TCAGACGGAG CCCTGCATCC TCAACAGCAC CTGCTTCAAT 
TACCACTACA ATGTTTCCAG CTGGAGCACC TGTCAGCTGA GCGAACATGC CATCTGCGGC 
CAGGGCACGA GGACACGACT CTTAGATTGC ATCCGCAGCG ATGGCAAAGT GGTGGATTTG 
AGCGTGTGCG AGGAGTTGGG CCTGCTTCAG CTGTGGAGAC TGACCATGAA ATGTGTGGTG 
GACTGTCCCT TTAACTGCAT CCTGTCTGAC TGGACGTCCT GGACGGAGTG TTCACACACC 
TGTGGAAGTA AAGGTGTTAA AACTCGCTTT CGCACTGTGC TCCAGCCGGC TCATGAGGAG 
GGACGTCCCT GCGCCTCCCA GCTGTCCCAA ACCAAGCCTT GTCCCATCAG CCCCTGCTAC 
ACGTGGCTAC TGAGCGACTG GTCTCCCTGC AGAGTCCAGG GAGCTGAATG TGGAGAGGGC 
ACGAGGGAGA GGAACCTGAC GTGTGTACAG CACTGGTCAG ACTGGCCGGA CAGTCCAAAC 
CCTTCTGAAG CCCTCGAGGA CGATAAGTGT GGAGACCGAC TCCGAAAGCA GAGTGAGCAG 
GAGCTGCAGC AGCCGTGCTT TGTTCCCTGC CCAGGAGACT GTCACCTGAC GGAGTGGTCA 
TTGTGGAGCT CCTGTCAGCT CACGTGTTTG GAGGGTCGCA GTTTCGAGAC AGTGGGGCGT 
CAGGCACGTT CACAAGCTGT TGTCATTCAG GTGCTGGACA ATCAGGACAG CTGCCCTCAG 
CAGGTTTTCG AGACCAGGCC GTGCAAAGGA GGGAAATGTC ACAGTTACGA GTGGAGGACC 
AGTGCCTGGA ATGACAACGA GAGATCAGTG TGGTGCCAGC GGTCTGACGG GGTCAATGTG 
ACAGGGGGCT GCTTTCCACA GAACCGGCCA ACCACTGTGC GCCACTGTCA CCCTCCCTGC 
ACTAAACCCT TCTCCCACTG CAAGCAGAGT GGTGTGTGTG GCTGTGAGAG AGGCTACACT 
GAAGTGATGA CCACACATGG CTTTTTGGAC TACTGCACCA AAACCCCAGG CACCGACAAC 
AAGAAGGCCG ATGTGAAGAC CAGCTCAGGA AGATTAAGAC CAGGCACGAC TCAAATCCAG 
GACTTCTTCG GCGAGTGGTC CCTGCAGCCT CTCGGCCCAG ATGGAAGAAT AAAGCTGTGG 
GTCTACGGGT TAACAGCTGG AGGATTCATT GTCATATTAT TCACAATTGC TGTGGCCTTT 
CTGTTTTGCA AACCATCCAA ACAGAGTAAA AGTGCATCTC CACCACAGAA GCCCTTGACT 
CTGGCCTATG ATGGCGATGT GGACATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

thsd7ba ( XM_689568.9 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_694660.6 Danio rerio thrombospondin, type I, domain containing 7Ba (thsd7ba), transcript variant X1, mRNA.

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CloneID	ODa14940
Clone ID Related Accession (Same CDS sequence)	XM_689568.8 , XM_689568.9
Accession Version	XM_689568.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4890bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	thrombospondin type-1 domain-containing protein 7B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_689568.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAATGG GCTGGACTAT GATTCTAAAA GATGACTCTG GTGTAACGGG CTGGACATGG 
CGGGTTGGGA ACTACCTGCT GCTCTCAATG TCTCTCATCG GAATGCATGT GAAGTCAGAC 
CTGGAATTTC CAAAGGACAG GCACTATACA TGGAAAACGG GTCATTGGGG CAGATGCATA 
GGGGAAGAAT GTGGTACCGG CGGGGTACAA TCCAGGACAG TGTGGTGCAT CCACACAGAA 
GGCTGGACCA CACACCACTC CAACTGTCGG CATTCAGACA AACCCGAGAG CCAGAGGCCT 
TGCTTTAAAG TATGTGAGTG GCACCAGGAC CTCTTTGAAT GGGAAGTGTC AGAATGGGGC 
CCTTGTGTTC TAGCCCCCCT CCTGTCCAAC GAGCTCAAAC TGCGGACCGC ACAGTGCATT 
ACAGCCCAAC ACGGCATCCA GAGACGGAAG GTCCATTGTG TGCGCACTTC CAACCGCACA 
TCGGTTACAG AAAGGATATG CGATTTCTTT TCACCACGAC CAGCTTTCGA GCAGGCCTGC 
TTGATCCCCT GCCCTCATGA CTGCATTGTG TCCGATTTCT CCTCTTGGTC TGGCTGCAGC 
AGAACATGCG GTGCGGGTCT TCAACATCGA ACCCGACATG TCCTAGCAGC GCCAATGTAC 
GGAGGCGCCA ACTGCCCCAA CCTCACCCAA ACAAGGACTT GCTCAAACCT TTTCCCCTGT 
CCTCTTGGGG AAAGCGAGCA TCACTACAGC CTTAAAGTCG GCCTCTGGAG CGAATGCAGG 
CAGCCACAAC ACAAAGGGCT TTGGATGAGC GGAAGGACCG TGCTAGACTT CAGCACAGGA 
GATACCGAAA GAAACACAGT GAAACGACAT ATTCAGAGCT CCCAGCACCA CCATCACCAC 
CTCCACCAAC ACCACCACCA GCACAGTCTC AAGGGTTTAG ATGTGGAGAT CGGCTATCAA 
ACACGGCAAG TTCGTTGCAT GCGCAGCGAT GGAAAAAACG CCATGCTGAG CCTTTGTACC 
CTTGACAACG GCCCTGGCAC TTTCCAGTCT TGTGTTATGC CCAGAGACTG CGAGACCTCC 
GATTGGTCCG TTTGGAGTCC ATGTTCAAAG ACATGCAGAG CATCAGATAT GTCGCCTGGT 
TTTCGGGTCA GGACTCGAAG CATAACGCAC ACTCCCATTG GTCAAGGCAA AGGATGCCCT 
GCTCTTGAAG AAAAGGAGGC CTGCAACATC ATTGGAGATC TGCTTCCAAA CTGCCCAAGG 
TACGTGTGGA AGAGCACCGA TTGGGGGGAC TGTCAAGTGG CCCCTCTTCT TAGCCAGCAG 
GACAGAAGGC TTGACAATGT TAGCGTTCTC TGTGGTGGAG GGATTCAGAC CAGGCAGCTG 
TACTGCGTCC AGGTCCCAGA CAATTCCACC CCTCATCATA GAAAAGAGGT GTCTAGGCCT 
GTAAATGTGC ATCTGTGCAC AGGAGATCCG CCTTCTGCAG TGCAGCCCTG CTCCATTCCA 
TGCCCACAGC TGTGCCTGCT CTCATCCTGG TCCTCCTGGG GAGCCTGTAT CCACGACAAC 
TGCATGGAGA CGCATGGACG AAAAGGCTTC AAGCAGAGGA GGAGGGATGT GCTGGAGGAA 
GGATCTGGAA CCGAGAACTG TGCTCATCTC CTGGAGTCAA TGCCCTGTGA GGATCCACTG 
TGCTTCCGGT GGCAGGTGCA GATGGAGAGT GCTTGTGTGC CCAGCAATGG CACCTGCGGG 
AATGGAACCA GGACACAAAC TGTGGCTTGC ATCAATTCAC AAGGGCAGCT TGTTCCAGAG 
GAGCTTTGTG AAGAAGAGTC TCCCCCTGCT GAGGTGTCCT GTGTGATGGC CTGTTCTGGT 
GACTGTGTGC TCAGCTCCTG GTCTCACTGG TCCTCCTGCT CTCACAGCTG CTCCAGCAAA 
AACTCTGAAG GCCGTCAGAG CCGCATACGC ACCATATTAG GCTTGCCAGG AGAAGATAAG 
ACGTGTCCAG CTGCTACTGC TCTGGAGGAG TGGAGAACTT GTAATGATCA TCCATGCATC 
ACCTTCTTCT GGGAAGCTTC GCCGTGGGGG CCTTGCATTG AAGACTCCTC TATGAATGTA 
AATGGCAGCG GCTACTGGAA CGGAACGGCC ACCTGTGCAG TCGGGCTGCA GATCCGTAAA 
GTGGCCTGCA TGAAAATGGG CGTTGGGCCG GTTATTCCCA AAAGGTGTCC AGAATCTGCC 
CGTCCAGACA CCATTCAGCC CTGTTTACTG CCCTGTGAGA AAGATTGCAT TGTGACTCCT 
TTCAGCGAAT GGACAGCCTG CCCGACCACA TGCCTGCCAG TGAACAGCAG CGTGGCCTCA 
CAGTCACGAT ACAGGATCAT CATCCAGCGG GCGGCCAATG GAGGTCAAGA GTGTCCCGAT 
ACTTTATTTG AAGAGAGGGA ATGTGAAGCT CTTCTGCTTT GTCCCACTTA CAGATGGAAG 
ACACACAGAT GGCATCCGTG TACTCTGGTC CCAGATTCAG TCCAGCAGGG AATGACCGGA 
GCGAGTGAAT CGTGTGGTAA AGGATTAGAG ATGAGAGGCG TGAGCTGTGT GAATGAGTAT 
GATGAGATGG TGGCCGTGTC CGAGTGTCTC CAGTGGGCCG GGCCGATGCC GTCTCGCTTC 
AGGAGCTGTT GGGTGGCATG TAAAGATGAC TGTGCCTTTA GTTCCTGGTC GAAATTTTCT 
GAATGCGCTG GATGTGGGAG CAGACGGACA CGCAAACGCA CAATTACAGG TCGTAGCCGA 
AAACTAGACC GATGTCAGCG GGAAGACTTG TTCCCTCTTG TGGAGTCCGA ACCCTGTCCG 
TGTGAGGAGT TTGTTTCTCA GCCATACGGT AATTGGTCGT CTTGTATCCT GTCGGAGACC 
CCAGCACAGG GTTGGAGGGG TCGTCGAGAG GCACGCGACT GTGGTCAGGG ATTCCGCTAT 
CGAGCAATGT CCTGTCTAGA CCACGAATCC CACCTGGTCA GCCCAGAACT CTGCAGCGAA 
TCAGGTCTGG TGGAAGAGGT CTGCTACATA CCCTGCCCTC TGGACTGCAA GCTGAGCGAC 
TGGTCACCGT GGTCAGCCTG CAGCGCCTCC TGTGGCAGTG GACTGAAAAT CAGGTCCAAA 
TGGCTACGAG AGAAGCCTTT CAACGGGGGG AGGCCGTGTC CAAAACTAGA CCTCAAGAAT 
CAGGCTCAGG TGTATGAGGC AGTTCCCTGT CGAAGTGAGT GTGATCAGTA CGAGTGGCTA 
ACAGACCCAT GGTCAGTCTG TACCATCAAC ACAGTGGATG AGCTGCCAGC CTGTGGAGAG 
GGAGTCCAGA GCCGCAAGAT CTGGTGTGTG AGTCGTGGCA GTGAGGACCT GGTGGCCAGT 
GTGAATGAGA CCCTGTGTGA CCAGGATGAA ATTCCTCCTC AAGCGCAGAC CTGCGTCCTG 
CCGTGTCCTG ACGACTGCGT GATGTCCCAG TGGAGTCAGT GGAGCACCTG TGCCATACCG 
TGTGACGAAA ACAGCTCGAG GACTAGGACA AGGCACGTGC TGCGCCCCCC GGTGGCTGAC 
AGAGTGTGTC CAGAAAATAA TCAGACGGAG CCCTGCATCC TCAACAGCAC CTGCTTCAAT 
TACCACTACA ATGTTTCCAG CTGGAGCACC TGTCAGCTGA GCGAACATGC CATCTGCGGC 
CAGGGCACGA GGACACGACT CTTAGATTGC ATCCGCAGCG ATGGCAAAGT GGTGGATTTG 
AGCGTGTGCG AGGAGTTGGG CCTGCTTCAG CTGTGGAGAC TGACCATGAA ATGTGTGGTG 
GACTGTCCCT TTAACTGCAT CCTGTCTGAC TGGACGTCCT GGACGGAGTG TTCACACACC 
TGTGGAAGTA AAGGTGTTAA AACTCGCTTT CGCACTGTGC TCCAGCCGGC TCATGAGGAG 
GGACGTCCCT GCGCCTCCCA GCTGTCCCAA ACCAAGCCTT GTCCCATCAG CCCCTGCTAC 
ACGTGGCTAC TGAGCGACTG GTCTCCCTGC AGAGTCCAGG GAGCTGAATG TGGAGAGGGC 
ACGAGGGAGA GGAACCTGAC GTGTGTACAG CACTGGTCAG ACTGGCCGGA CAGTCCAAAC 
CCTTCTGAAG CCCTCGAGGA CGATAAGTGT GGAGACCGAC TCCGAAAGCA GAGTGAGCAG 
GAGCTGCAGC AGCCGTGCTT TGTTCCCTGC CCAGGAGACT GTCACCTGAC GGAGTGGTCA 
TTGTGGAGCT CCTGTCAGCT CACGTGTTTG GAGGGTCGCA GTTTCGAGAC AGTGGGGCGT 
CAGGCACGTT CACAAGCTGT TGTCATTCAG GTGCTGGACA ATCAGGACAG CTGCCCTCAG 
CAGGTTTTCG AGACCAGGCC GTGCAAAGGA GGGAAATGTC ACAGTTACGA GTGGAGGACC 
AGTGCCTGGA ATGACAACGA GAGATCAGTG TGGTGCCAGC GGTCTGACGG GGTCAATGTG 
ACAGGGGGCT GCTTTCCACA GAACCGGCCA ACCACTGTGC GCCACTGTCA CCCTCCCTGC 
ACTAAACCCT TCTCCCACTG CAAGCAGAGT GGTGTGTGTG GCTGTGAGAG AGGCTACACT 
GAAGTGATGA CCACACATGG CTTTTTGGAC TACTGCACCA AAACCCCAGG CACCGACAAC 
AAGAAGGCCG ATGTGAAGAC CAGCTCAGGA AGATTAAGAC CAGGCACGAC TCAAATCCAG 
GACTTCTTCG GCGAGTGGTC CCTGCAGCCT CTCGGCCCAG ATGGAAGAAT AAAGCTGTGG 
GTCTACGGGT TAACAGCTGG AGGATTCATT GTCATATTAT TCACAATTGC TGTGGCCTTT 
CTGTTTTGCA AACCATCCAA ACAGAGTAAA AGTGCATCTC CACCACAGAA GCCCTTGACT 
CTGGCCTATG ATGGCGATGT GGACATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_694660.6
CDS	532..5421
Translation	MSMGWTMILKDDSGVTGWTWRVGNYLLLSMSLIGMHVKSDLEFPKDRHYTWKTGHWGRCIGEECGTGGVQSRTVWCIHTEGWTTHHSNCRHSDKPESQRPCFKVCEWHQDLFEWEVSEWGPCVLAPLLSNELKLRTAQCITAQHGIQRRKVHCVRTSNRTSVTERICDFFSPRPAFEQACLIPCPHDCIVSDFSSWSGCSRTCGAGLQHRTRHVLAAPMYGGANCPNLTQTRTCSNLFPCPLGESEHHYSLKVGLWSECRQPQHKGLWMSGRTVLDFSTGDTERNTVKRHIQSSQHHHHHLHQHHHQHSLKGLDVEIGYQTRQVRCMRSDGKNAMLSLCTLDNGPGTFQSCVMPRDCETSDWSVWSPCSKTCRASDMSPGFRVRTRSITHTPIGQGKGCPALEEKEACNIIGDLLPNCPRYVWKSTDWGDCQVAPLLSQQDRRLDNVSVLCGGGIQTRQLYCVQVPDNSTPHHRKEVSRPVNVHLCTGDPPSAVQPCSIPCPQLCLLSSWSSWGACIHDNCMETHGRKGFKQRRRDVLEEGSGTENCAHLLESMPCEDPLCFRWQVQMESACVPSNGTCGNGTRTQTVACINSQGQLVPEELCEEESPPAEVSCVMACSGDCVLSSWSHWSSCSHSCSSKNSEGRQSRIRTILGLPGEDKTCPAATALEEWRTCNDHPCITFFWEASPWGPCIEDSSMNVNGSGYWNGTATCAVGLQIRKVACMKMGVGPVIPKRCPESARPDTIQPCLLPCEKDCIVTPFSEWTACPTTCLPVNSSVASQSRYRIIIQRAANGGQECPDTLFEERECEALLLCPTYRWKTHRWHPCTLVPDSVQQGMTGASESCGKGLEMRGVSCVNEYDEMVAVSECLQWAGPMPSRFRSCWVACKDDCAFSSWSKFSECAGCGSRRTRKRTITGRSRKLDRCQREDLFPLVESEPCPCEEFVSQPYGNWSSCILSETPAQGWRGRREARDCGQGFRYRAMSCLDHESHLVSPELCSESGLVEEVCYIPCPLDCKLSDWSPWSACSASCGSGLKIRSKWLREKPFNGGRPCPKLDLKNQAQVYEAVPCRSECDQYEWLTDPWSVCTINTVDELPACGEGVQSRKIWCVSRGSEDLVASVNETLCDQDEIPPQAQTCVLPCPDDCVMSQWSQWSTCAIPCDENSSRTRTRHVLRPPVADRVCPENNQTEPCILNSTCFNYHYNVSSWSTCQLSEHAICGQGTRTRLLDCIRSDGKVVDLSVCEELGLLQLWRLTMKCVVDCPFNCILSDWTSWTECSHTCGSKGVKTRFRTVLQPAHEEGRPCASQLSQTKPCPISPCYTWLLSDWSPCRVQGAECGEGTRERNLTCVQHWSDWPDSPNPSEALEDDKCGDRLRKQSEQELQQPCFVPCPGDCHLTEWSLWSSCQLTCLEGRSFETVGRQARSQAVVIQVLDNQDSCPQQVFETRPCKGGKCHSYEWRTSAWNDNERSVWCQRSDGVNVTGGCFPQNRPTTVRHCHPPCTKPFSHCKQSGVCGCERGYTEVMTTHGFLDYCTKTPGTDNKKADVKTSSGRLRPGTTQIQDFFGEWSLQPLGPDGRIKLWVYGLTAGGFIVILFTIAVAFLFCKPSKQSKSASPPQKPLTLAYDGDVDM

Target ORF information:

RefSeq Version	XM_689568.9
Organism	Danio rerio(zebrafish)
Definition	Danio rerio thrombospondin, type I, domain containing 7Ba (thsd7ba), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_689568.9

ORF Insert Sequence:

ATGTCAATGG GCTGGACTAT GATTCTAAAA GATGACTCTG GTGTAACGGG CTGGACATGG 
CGGGTTGGGA ACTACCTGCT GCTCTCAATG TCTCTCATCG GAATGCATGT GAAGTCAGAC 
CTGGAATTTC CAAAGGACAG GCACTATACA TGGAAAACGG GTCATTGGGG CAGATGCATA 
GGGGAAGAAT GTGGTACCGG CGGGGTACAA TCCAGGACAG TGTGGTGCAT CCACACAGAA 
GGCTGGACCA CACACCACTC CAACTGTCGG CATTCAGACA AACCCGAGAG CCAGAGGCCT 
TGCTTTAAAG TATGTGAGTG GCACCAGGAC CTCTTTGAAT GGGAAGTGTC AGAATGGGGC 
CCTTGTGTTC TAGCCCCCCT CCTGTCCAAC GAGCTCAAAC TGCGGACCGC ACAGTGCATT 
ACAGCCCAAC ACGGCATCCA GAGACGGAAG GTCCATTGTG TGCGCACTTC CAACCGCACA 
TCGGTTACAG AAAGGATATG CGATTTCTTT TCACCACGAC CAGCTTTCGA GCAGGCCTGC 
TTGATCCCCT GCCCTCATGA CTGCATTGTG TCCGATTTCT CCTCTTGGTC TGGCTGCAGC 
AGAACATGCG GTGCGGGTCT TCAACATCGA ACCCGACATG TCCTAGCAGC GCCAATGTAC 
GGAGGCGCCA ACTGCCCCAA CCTCACCCAA ACAAGGACTT GCTCAAACCT TTTCCCCTGT 
CCTCTTGGGG AAAGCGAGCA TCACTACAGC CTTAAAGTCG GCCTCTGGAG CGAATGCAGG 
CAGCCACAAC ACAAAGGGCT TTGGATGAGC GGAAGGACCG TGCTAGACTT CAGCACAGGA 
GATACCGAAA GAAACACAGT GAAACGACAT ATTCAGAGCT CCCAGCACCA CCATCACCAC 
CTCCACCAAC ACCACCACCA GCACAGTCTC AAGGGTTTAG ATGTGGAGAT CGGCTATCAA 
ACACGGCAAG TTCGTTGCAT GCGCAGCGAT GGAAAAAACG CCATGCTGAG CCTTTGTACC 
CTTGACAACG GCCCTGGCAC TTTCCAGTCT TGTGTTATGC CCAGAGACTG CGAGACCTCC 
GATTGGTCCG TTTGGAGTCC ATGTTCAAAG ACATGCAGAG CATCAGATAT GTCGCCTGGT 
TTTCGGGTCA GGACTCGAAG CATAACGCAC ACTCCCATTG GTCAAGGCAA AGGATGCCCT 
GCTCTTGAAG AAAAGGAGGC CTGCAACATC ATTGGAGATC TGCTTCCAAA CTGCCCAAGG 
TACGTGTGGA AGAGCACCGA TTGGGGGGAC TGTCAAGTGG CCCCTCTTCT TAGCCAGCAG 
GACAGAAGGC TTGACAATGT TAGCGTTCTC TGTGGTGGAG GGATTCAGAC CAGGCAGCTG 
TACTGCGTCC AGGTCCCAGA CAATTCCACC CCTCATCATA GAAAAGAGGT GTCTAGGCCT 
GTAAATGTGC ATCTGTGCAC AGGAGATCCG CCTTCTGCAG TGCAGCCCTG CTCCATTCCA 
TGCCCACAGC TGTGCCTGCT CTCATCCTGG TCCTCCTGGG GAGCCTGTAT CCACGACAAC 
TGCATGGAGA CGCATGGACG AAAAGGCTTC AAGCAGAGGA GGAGGGATGT GCTGGAGGAA 
GGATCTGGAA CCGAGAACTG TGCTCATCTC CTGGAGTCAA TGCCCTGTGA GGATCCACTG 
TGCTTCCGGT GGCAGGTGCA GATGGAGAGT GCTTGTGTGC CCAGCAATGG CACCTGCGGG 
AATGGAACCA GGACACAAAC TGTGGCTTGC ATCAATTCAC AAGGGCAGCT TGTTCCAGAG 
GAGCTTTGTG AAGAAGAGTC TCCCCCTGCT GAGGTGTCCT GTGTGATGGC CTGTTCTGGT 
GACTGTGTGC TCAGCTCCTG GTCTCACTGG TCCTCCTGCT CTCACAGCTG CTCCAGCAAA 
AACTCTGAAG GCCGTCAGAG CCGCATACGC ACCATATTAG GCTTGCCAGG AGAAGATAAG 
ACGTGTCCAG CTGCTACTGC TCTGGAGGAG TGGAGAACTT GTAATGATCA TCCATGCATC 
ACCTTCTTCT GGGAAGCTTC GCCGTGGGGG CCTTGCATTG AAGACTCCTC TATGAATGTA 
AATGGCAGCG GCTACTGGAA CGGAACGGCC ACCTGTGCAG TCGGGCTGCA GATCCGTAAA 
GTGGCCTGCA TGAAAATGGG CGTTGGGCCG GTTATTCCCA AAAGGTGTCC AGAATCTGCC 
CGTCCAGACA CCATTCAGCC CTGTTTACTG CCCTGTGAGA AAGATTGCAT TGTGACTCCT 
TTCAGCGAAT GGACAGCCTG CCCGACCACA TGCCTGCCAG TGAACAGCAG CGTGGCCTCA 
CAGTCACGAT ACAGGATCAT CATCCAGCGG GCGGCCAATG GAGGTCAAGA GTGTCCCGAT 
ACTTTATTTG AAGAGAGGGA ATGTGAAGCT CTTCTGCTTT GTCCCACTTA CAGATGGAAG 
ACACACAGAT GGCATCCGTG TACTCTGGTC CCAGATTCAG TCCAGCAGGG AATGACCGGA 
GCGAGTGAAT CGTGTGGTAA AGGATTAGAG ATGAGAGGCG TGAGCTGTGT GAATGAGTAT 
GATGAGATGG TGGCCGTGTC CGAGTGTCTC CAGTGGGCCG GGCCGATGCC GTCTCGCTTC 
AGGAGCTGTT GGGTGGCATG TAAAGATGAC TGTGCCTTTA GTTCCTGGTC GAAATTTTCT 
GAATGCGCTG GATGTGGGAG CAGACGGACA CGCAAACGCA CAATTACAGG TCGTAGCCGA 
AAACTAGACC GATGTCAGCG GGAAGACTTG TTCCCTCTTG TGGAGTCCGA ACCCTGTCCG 
TGTGAGGAGT TTGTTTCTCA GCCATACGGT AATTGGTCGT CTTGTATCCT GTCGGAGACC 
CCAGCACAGG GTTGGAGGGG TCGTCGAGAG GCACGCGACT GTGGTCAGGG ATTCCGCTAT 
CGAGCAATGT CCTGTCTAGA CCACGAATCC CACCTGGTCA GCCCAGAACT CTGCAGCGAA 
TCAGGTCTGG TGGAAGAGGT CTGCTACATA CCCTGCCCTC TGGACTGCAA GCTGAGCGAC 
TGGTCACCGT GGTCAGCCTG CAGCGCCTCC TGTGGCAGTG GACTGAAAAT CAGGTCCAAA 
TGGCTACGAG AGAAGCCTTT CAACGGGGGG AGGCCGTGTC CAAAACTAGA CCTCAAGAAT 
CAGGCTCAGG TGTATGAGGC AGTTCCCTGT CGAAGTGAGT GTGATCAGTA CGAGTGGCTA 
ACAGACCCAT GGTCAGTCTG TACCATCAAC ACAGTGGATG AGCTGCCAGC CTGTGGAGAG 
GGAGTCCAGA GCCGCAAGAT CTGGTGTGTG AGTCGTGGCA GTGAGGACCT GGTGGCCAGT 
GTGAATGAGA CCCTGTGTGA CCAGGATGAA ATTCCTCCTC AAGCGCAGAC CTGCGTCCTG 
CCGTGTCCTG ACGACTGCGT GATGTCCCAG TGGAGTCAGT GGAGCACCTG TGCCATACCG 
TGTGACGAAA ACAGCTCGAG GACTAGGACA AGGCACGTGC TGCGCCCCCC GGTGGCTGAC 
AGAGTGTGTC CAGAAAATAA TCAGACGGAG CCCTGCATCC TCAACAGCAC CTGCTTCAAT 
TACCACTACA ATGTTTCCAG CTGGAGCACC TGTCAGCTGA GCGAACATGC CATCTGCGGC 
CAGGGCACGA GGACACGACT CTTAGATTGC ATCCGCAGCG ATGGCAAAGT GGTGGATTTG 
AGCGTGTGCG AGGAGTTGGG CCTGCTTCAG CTGTGGAGAC TGACCATGAA ATGTGTGGTG 
GACTGTCCCT TTAACTGCAT CCTGTCTGAC TGGACGTCCT GGACGGAGTG TTCACACACC 
TGTGGAAGTA AAGGTGTTAA AACTCGCTTT CGCACTGTGC TCCAGCCGGC TCATGAGGAG 
GGACGTCCCT GCGCCTCCCA GCTGTCCCAA ACCAAGCCTT GTCCCATCAG CCCCTGCTAC 
ACGTGGCTAC TGAGCGACTG GTCTCCCTGC AGAGTCCAGG GAGCTGAATG TGGAGAGGGC 
ACGAGGGAGA GGAACCTGAC GTGTGTACAG CACTGGTCAG ACTGGCCGGA CAGTCCAAAC 
CCTTCTGAAG CCCTCGAGGA CGATAAGTGT GGAGACCGAC TCCGAAAGCA GAGTGAGCAG 
GAGCTGCAGC AGCCGTGCTT TGTTCCCTGC CCAGGAGACT GTCACCTGAC GGAGTGGTCA 
TTGTGGAGCT CCTGTCAGCT CACGTGTTTG GAGGGTCGCA GTTTCGAGAC AGTGGGGCGT 
CAGGCACGTT CACAAGCTGT TGTCATTCAG GTGCTGGACA ATCAGGACAG CTGCCCTCAG 
CAGGTTTTCG AGACCAGGCC GTGCAAAGGA GGGAAATGTC ACAGTTACGA GTGGAGGACC 
AGTGCCTGGA ATGACAACGA GAGATCAGTG TGGTGCCAGC GGTCTGACGG GGTCAATGTG 
ACAGGGGGCT GCTTTCCACA GAACCGGCCA ACCACTGTGC GCCACTGTCA CCCTCCCTGC 
ACTAAACCCT TCTCCCACTG CAAGCAGAGT GGTGTGTGTG GCTGTGAGAG AGGCTACACT 
GAAGTGATGA CCACACATGG CTTTTTGGAC TACTGCACCA AAACCCCAGG CACCGACAAC 
AAGAAGGCCG ATGTGAAGAC CAGCTCAGGA AGATTAAGAC CAGGCACGAC TCAAATCCAG 
GACTTCTTCG GCGAGTGGTC CCTGCAGCCT CTCGGCCCAG ATGGAAGAAT AAAGCTGTGG 
GTCTACGGGT TAACAGCTGG AGGATTCATT GTCATATTAT TCACAATTGC TGTGGCCTTT 
CTGTTTTGCA AACCATCCAA ACAGAGTAAA AGTGCATCTC CACCACAGAA GCCCTTGACT 
CTGGCCTATG ATGGCGATGT GGACATGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

thsd7ba ( XM_009304519.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302794.1 Danio rerio thrombospondin, type I, domain containing 7Ba (thsd7ba), transcript variant X2, mRNA.

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CloneID	ODa44838
Clone ID Related Accession (Same CDS sequence)	XM_009304519.2 , XM_009304519.3
Accession Version	XM_009304519.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	thrombospondin type-1 domain-containing protein 7B isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304519.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAATGG GCTGGACTAT GATTCTAAAA GATGACTCTG GTGTAACGGG CTGGACATGG 
CGGGTTGGGA ACTACCTGCT GCTCTCAATG TCTCTCATCG GAATGCATGT GAAGTCAGAC 
CTGGAATTTC CAAAGGACAG GCACTATACA TGGAAAACGG GTCATTGGGG CAGATGCATA 
GGGGAAGAAT GTGGTACCGG CGGGGTACAA TCCAGGACAG TGTGGTGCAT CCACACAGAA 
GGCTGGACCA CACACCACTC CAACTGTCGG CATTCAGACA AACCCGAGAG CCAGAGGCCT 
TGCTTTAAAG TATGTGAGTG GCACCAGGAC CTCTTTGAAT GGGAAGTGTC AGAATGGGGC 
CCTTGTGTTC TAGCCCCCCT CCTGTCCAAC GAGCTCAAAC TGCGGACCGC ACAGTGCATT 
ACAGCCCAAC ACGGCATCCA GAGACGGAAG GTCCATTGTG TGCGCACTTC CAACCGCACA 
TCGGTTACAG AAAGGATATG CGATTTCTTT TCACCACGAC CAGCTTTCGA GCAGGCCTGC 
TTGATCCCCT GCCCTCATGA CTGCATTGTG TCCGATTTCT CCTCTTGGTC TGGCTGCAGC 
AGAACATGCG GTGCGGGTCT TCAACATCGA ACCCGACATG TCCTAGCAGC GCCAATGTAC 
GGAGGCGCCA ACTGCCCCAA CCTCACCCAA ACAAGGACTT GCTCAAACCT TTTCCCCTGT 
CCTCTTGGGG AAAGCGAGCA TCACTACAGC CTTAAAGTCG GCCTCTGGAG CGAATGCAGG 
CAGCCACAAC ACAAAGGGCT TTGGATGAGC GGAAGGACCG TGCTAGACTT CAGCACAGGA 
GATACCGAAA GAAACACAGT GAAACGACAT ATTCAGAGCT CCCAGCACCA CCATCACCAC 
CTCCACCAAC ACCACCACCA GCACAGTCTC AAGGGTTTAG ATGTGGAGAT CGGCTATCAA 
ACACGGCAAG TTCGTTGCAT GCGCAGCGAT GGAAAAAACG CCATGCTGAG CCTTTGTACC 
CTTGACAACG GCCCTGGCAC TTTCCAGTCT TGTGTTATGC CCAGAGACTG CGAGACCTCC 
GATTGGTCCG TTTGGAGTCC ATGTTCAAAG ACATGCAGAG CATCAGATAT GTCGCCTGGT 
TTTCGGGTCA GGACTCGAAG CATAACGCAC ACTCCCATTG GTCAAGGCAA AGGATGCCCT 
GCTCTTGAAG AAAAGGAGGC CTGCAACATC ATTGGAGATC TGCTTCCAAA CTGCCCAAGG 
TACGTGTGGA AGAGCACCGA TTGGGGGGAC TGTCAAGTGG CCCCTCTTCT TAGCCAGCAG 
GACAGAAGGC TTGACAATGT TAGCGTTCTC TGTGGTGGAG GGATTCAGAC CAGGCAGCTG 
TACTGCGTCC AGGTCCCAGA CAATTCCACC CCTCATCATA GAAAAGAGGT GTCTAGGCCT 
GTAAATGTGC ATCTGTGCAC AGGAGATCCG CCTTCTGCAG TGCAGCCCTG CTCCATTCCA 
TGCCCACAGC TGTGCCTGCT CTCATCCTGG TCCTCCTGGG GAGCCTGTAT CCACGACAAC 
TGCATGGAGA CGCATGGACG AAAAGGCTTC AAGCAGAGGA GGAGGGATGT GCTGGAGGAA 
GGATCTGGAA CCGAGAACTG TGCTCATCTC CTGGAGTCAA TGCCCTGTGA GGATCCACTG 
TGCTTCCGGT GGCAGGTGCA GATGGAGAGT GCTTGTGTGC CCAGCAATGG CACCTGCGGG 
AATGGAACCA GGACACAAAC TGTGGCTTGC ATCAATTCAC AAGGGCAGCT TGTTCCAGAG 
GAGCTTTGTG AAGAAGAGTC TCCCCCTGCT GAGGTGTCCT GTGTGATGGC CTGTTCTGGT 
GACTGTGTGC TCAGCTCCTG GTCTCACTGG TCCTCCTGCT CTCACAGCTG CTCCAGCAAA 
AACTCTGAAG GCCGTCAGAG CCGCATACGC ACCATATTAG GCTTGCCAGG AGAAGATAAG 
ACGTGTCCAG CTGCTACTGC TCTGGAGGAG TGGAGAACTT GTAATGATCA TCCATGCATC 
ACCTTCTTCT GGGAAGCTTC GCCGTGGGGG CCTTGCATTG AAGACTCCTC TATGAATGTA 
AATGGCAGCG GCTACTGGAA CGGAACGGCC ACCTGTGCAG TCGGGCTGCA GATCCGTAAA 
GTGGCCTGCA TGAAAATGGG CGTTGGGCCG GTTATTCCCA AAAGGTGTCC AGAATCTGCC 
CGTCCAGACA CCATTCAGCC CTGTTTACTG CCCTGTGAGA AAGATTGCAT TGTGACTCCT 
TTCAGCGAAT GGACAGCCTG CCCGACCACA TGCCTGCCAG TGAACAGCAG CGTGGCCTCA 
CAGTCACGAT ACAGGATCAT CATCCAGCGG GCGGCCAATG GAGGTCAAGA GTGTCCCGAT 
ACTTTATTTG AAGAGAGGGA ATGTGAAGCT CTTCTGCTTT GTCCCACTTA CAGATGGAAG 
ACACACAGAT GGCATCCGTG TACTCTGGTC CCAGATTCAG TCCAGCAGGG AATGACCGGA 
GCGAGTGAAT CGTGTGGTAA AGGATTAGAG ATGAGAGGCG TGAGCTGTGT GAATGAGTAT 
GATGAGATGG TGGCCGTGTC CGAGTGTCTC CAGTGGGCCG GGCCGATGCC GTCTCGCTTC 
AGGAGCTGTT GGGTGGCATG TAAAGATGAC TGTGCCTTTA GTTCCTGGTC GAAATTTTCT 
GAATGCGCTG GATGTGGGAG CAGACGGACA CGCAAACGCA CAATTACAGG TCGTAGCCGA 
AAACTAGACC GATGTCAGCG GGAAGACTTG TTCCCTCTTG TGGAGTCCGA ACCCTGTCCG 
TGTGAGGAGT TTGTTTCTCA GCCATACGGT AATTGGTCGT CTTGTATCCT GTCGGAGACC 
CCAGCACAGG GTTGGAGGGG TCGTCGAGAG GCACGCGACT GTGGTCAGGG ATTCCGCTAT 
CGAGCAATGT CCTGTCTAGA CCACGAATCC CACCTGGTCA GCCCAGAACT CTGCAGCGAA 
TCAGGTCTGG TGGAAGAGGT CTGCTACATA CCCTGCCCTC TGGACTGCAA GCTGAGCGAC 
TGGTCACCGT GGTCAGCCTG CAGCGCCTCC TGTGGCAGTG GACTGAAAAT CAGGTCCAAA 
TGGCTACGAG AGAAGCCTTT CAACGGGGGG AGGCCGTGTC CAAAACTAGA CCTCAAGAAT 
CAGGTGTATG AGGCAGTTCC CTGTCGAAGT GAGTGTGATC AGTACGAGTG GCTAACAGAC 
CCATGGTCAG TCTGTACCAT CAACACAGTG GATGAGCTGC CAGCCTGTGG AGAGGGAGTC 
CAGAGCCGCA AGATCTGGTG TGTGAGTCGT GGCAGTGAGG ACCTGGTGGC CAGTGTGAAT 
GAGACCCTGT GTGACCAGGA TGAAATTCCT CCTCAAGCGC AGACCTGCGT CCTGCCGTGT 
CCTGACGACT GCGTGATGTC CCAGTGGAGT CAGTGGAGCA CCTGTGCCAT ACCGTGTGAC 
GAAAACAGCT CGAGGACTAG GACAAGGCAC GTGCTGCGCC CCCCGGTGGC TGACAGAGTG 
TGTCCAGAAA ATAATCAGAC GGAGCCCTGC ATCCTCAACA GCACCTGCTT CAATTACCAC 
TACAATGTTT CCAGCTGGAG CACCTGTCAG CTGAGCGAAC ATGCCATCTG CGGCCAGGGC 
ACGAGGACAC GACTCTTAGA TTGCATCCGC AGCGATGGCA AAGTGGTGGA TTTGAGCGTG 
TGCGAGGAGT TGGGCCTGCT TCAGCTGTGG AGACTGACCA TGAAATGTGT GGTGGACTGT 
CCCTTTAACT GCATCCTGTC TGACTGGACG TCCTGGACGG AGTGTTCACA CACCTGTGGA 
AGTAAAGGTG TTAAAACTCG CTTTCGCACT GTGCTCCAGC CGGCTCATGA GGAGGGACGT 
CCCTGCGCCT CCCAGCTGTC CCAAACCAAG CCTTGTCCCA TCAGCCCCTG CTACACGTGG 
CTACTGAGCG ACTGGTCTCC CTGCAGAGTC CAGGGAGCTG AATGTGGAGA GGGCACGAGG 
GAGAGGAACC TGACGTGTGT ACAGCACTGG TCAGACTGGC CGGACAGTCC AAACCCTTCT 
GAAGCCCTCG AGGACGATAA GTGTGGAGAC CGACTCCGAA AGCAGAGTGA GCAGGAGCTG 
CAGCAGCCGT GCTTTGTTCC CTGCCCAGGA GACTGTCACC TGACGGAGTG GTCATTGTGG 
AGCTCCTGTC AGCTCACGTG TTTGGAGGGT CGCAGTTTCG AGACAGTGGG GCGTCAGGCA 
CGTTCACAAG CTGTTGTCAT TCAGGTGCTG GACAATCAGG ACAGCTGCCC TCAGCAGGTT 
TTCGAGACCA GGCCGTGCAA AGGAGGGAAA TGTCACAGTT ACGAGTGGAG GACCAGTGCC 
TGGAATGACA ACGAGAGATC AGTGTGGTGC CAGCGGTCTG ACGGGGTCAA TGTGACAGGG 
GGCTGCTTTC CACAGAACCG GCCAACCACT GTGCGCCACT GTCACCCTCC CTGCACTAAA 
CCCTTCTCCC ACTGCAAGCA GAGTGGTGTG TGTGGCTGTG AGAGAGGCTA CACTGAAGTG 
ATGACCACAC ATGGCTTTTT GGACTACTGC ACCAAAACCC CAGGCACCGA CAACAAGAAG 
GCCGATGTGA AGACCAGCTC AGGAAGATTA AGACCAGGCA CGACTCAAAT CCAGGACTTC 
TTCGGCGAGT GGTCCCTGCA GCCTCTCGGC CCAGATGGAA GAATAAAGCT GTGGGTCTAC 
GGGTTAACAG CTGGAGGATT CATTGTCATA TTATTCACAA TTGCTGTGGC CTTTCTGTTT 
TGCAAACCAT CCAAACAGAG TAAAAGTGCA TCTCCACCAC AGAAGCCCTT GACTCTGGCC 
TATGATGGCG ATGTGGACAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302794.1
CDS	491..5374
Translation	MSMGWTMILKDDSGVTGWTWRVGNYLLLSMSLIGMHVKSDLEFPKDRHYTWKTGHWGRCIGEECGTGGVQSRTVWCIHTEGWTTHHSNCRHSDKPESQRPCFKVCEWHQDLFEWEVSEWGPCVLAPLLSNELKLRTAQCITAQHGIQRRKVHCVRTSNRTSVTERICDFFSPRPAFEQACLIPCPHDCIVSDFSSWSGCSRTCGAGLQHRTRHVLAAPMYGGANCPNLTQTRTCSNLFPCPLGESEHHYSLKVGLWSECRQPQHKGLWMSGRTVLDFSTGDTERNTVKRHIQSSQHHHHHLHQHHHQHSLKGLDVEIGYQTRQVRCMRSDGKNAMLSLCTLDNGPGTFQSCVMPRDCETSDWSVWSPCSKTCRASDMSPGFRVRTRSITHTPIGQGKGCPALEEKEACNIIGDLLPNCPRYVWKSTDWGDCQVAPLLSQQDRRLDNVSVLCGGGIQTRQLYCVQVPDNSTPHHRKEVSRPVNVHLCTGDPPSAVQPCSIPCPQLCLLSSWSSWGACIHDNCMETHGRKGFKQRRRDVLEEGSGTENCAHLLESMPCEDPLCFRWQVQMESACVPSNGTCGNGTRTQTVACINSQGQLVPEELCEEESPPAEVSCVMACSGDCVLSSWSHWSSCSHSCSSKNSEGRQSRIRTILGLPGEDKTCPAATALEEWRTCNDHPCITFFWEASPWGPCIEDSSMNVNGSGYWNGTATCAVGLQIRKVACMKMGVGPVIPKRCPESARPDTIQPCLLPCEKDCIVTPFSEWTACPTTCLPVNSSVASQSRYRIIIQRAANGGQECPDTLFEERECEALLLCPTYRWKTHRWHPCTLVPDSVQQGMTGASESCGKGLEMRGVSCVNEYDEMVAVSECLQWAGPMPSRFRSCWVACKDDCAFSSWSKFSECAGCGSRRTRKRTITGRSRKLDRCQREDLFPLVESEPCPCEEFVSQPYGNWSSCILSETPAQGWRGRREARDCGQGFRYRAMSCLDHESHLVSPELCSESGLVEEVCYIPCPLDCKLSDWSPWSACSASCGSGLKIRSKWLREKPFNGGRPCPKLDLKNQVYEAVPCRSECDQYEWLTDPWSVCTINTVDELPACGEGVQSRKIWCVSRGSEDLVASVNETLCDQDEIPPQAQTCVLPCPDDCVMSQWSQWSTCAIPCDENSSRTRTRHVLRPPVADRVCPENNQTEPCILNSTCFNYHYNVSSWSTCQLSEHAICGQGTRTRLLDCIRSDGKVVDLSVCEELGLLQLWRLTMKCVVDCPFNCILSDWTSWTECSHTCGSKGVKTRFRTVLQPAHEEGRPCASQLSQTKPCPISPCYTWLLSDWSPCRVQGAECGEGTRERNLTCVQHWSDWPDSPNPSEALEDDKCGDRLRKQSEQELQQPCFVPCPGDCHLTEWSLWSSCQLTCLEGRSFETVGRQARSQAVVIQVLDNQDSCPQQVFETRPCKGGKCHSYEWRTSAWNDNERSVWCQRSDGVNVTGGCFPQNRPTTVRHCHPPCTKPFSHCKQSGVCGCERGYTEVMTTHGFLDYCTKTPGTDNKKADVKTSSGRLRPGTTQIQDFFGEWSLQPLGPDGRIKLWVYGLTAGGFIVILFTIAVAFLFCKPSKQSKSASPPQKPLTLAYDGDVDM

Target ORF information:

RefSeq Version	XM_009304519.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio thrombospondin, type I, domain containing 7Ba (thsd7ba), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304519.2

ORF Insert Sequence:

ATGTCAATGG GCTGGACTAT GATTCTAAAA GATGACTCTG GTGTAACGGG CTGGACATGG 
CGGGTTGGGA ACTACCTGCT GCTCTCAATG TCTCTCATCG GAATGCATGT GAAGTCAGAC 
CTGGAATTTC CAAAGGACAG GCACTATACA TGGAAAACGG GTCATTGGGG CAGATGCATA 
GGGGAAGAAT GTGGTACCGG CGGGGTACAA TCCAGGACAG TGTGGTGCAT CCACACAGAA 
GGCTGGACCA CACACCACTC CAACTGTCGG CATTCAGACA AACCCGAGAG CCAGAGGCCT 
TGCTTTAAAG TATGTGAGTG GCACCAGGAC CTCTTTGAAT GGGAAGTGTC AGAATGGGGC 
CCTTGTGTTC TAGCCCCCCT CCTGTCCAAC GAGCTCAAAC TGCGGACCGC ACAGTGCATT 
ACAGCCCAAC ACGGCATCCA GAGACGGAAG GTCCATTGTG TGCGCACTTC CAACCGCACA 
TCGGTTACAG AAAGGATATG CGATTTCTTT TCACCACGAC CAGCTTTCGA GCAGGCCTGC 
TTGATCCCCT GCCCTCATGA CTGCATTGTG TCCGATTTCT CCTCTTGGTC TGGCTGCAGC 
AGAACATGCG GTGCGGGTCT TCAACATCGA ACCCGACATG TCCTAGCAGC GCCAATGTAC 
GGAGGCGCCA ACTGCCCCAA CCTCACCCAA ACAAGGACTT GCTCAAACCT TTTCCCCTGT 
CCTCTTGGGG AAAGCGAGCA TCACTACAGC CTTAAAGTCG GCCTCTGGAG CGAATGCAGG 
CAGCCACAAC ACAAAGGGCT TTGGATGAGC GGAAGGACCG TGCTAGACTT CAGCACAGGA 
GATACCGAAA GAAACACAGT GAAACGACAT ATTCAGAGCT CCCAGCACCA CCATCACCAC 
CTCCACCAAC ACCACCACCA GCACAGTCTC AAGGGTTTAG ATGTGGAGAT CGGCTATCAA 
ACACGGCAAG TTCGTTGCAT GCGCAGCGAT GGAAAAAACG CCATGCTGAG CCTTTGTACC 
CTTGACAACG GCCCTGGCAC TTTCCAGTCT TGTGTTATGC CCAGAGACTG CGAGACCTCC 
GATTGGTCCG TTTGGAGTCC ATGTTCAAAG ACATGCAGAG CATCAGATAT GTCGCCTGGT 
TTTCGGGTCA GGACTCGAAG CATAACGCAC ACTCCCATTG GTCAAGGCAA AGGATGCCCT 
GCTCTTGAAG AAAAGGAGGC CTGCAACATC ATTGGAGATC TGCTTCCAAA CTGCCCAAGG 
TACGTGTGGA AGAGCACCGA TTGGGGGGAC TGTCAAGTGG CCCCTCTTCT TAGCCAGCAG 
GACAGAAGGC TTGACAATGT TAGCGTTCTC TGTGGTGGAG GGATTCAGAC CAGGCAGCTG 
TACTGCGTCC AGGTCCCAGA CAATTCCACC CCTCATCATA GAAAAGAGGT GTCTAGGCCT 
GTAAATGTGC ATCTGTGCAC AGGAGATCCG CCTTCTGCAG TGCAGCCCTG CTCCATTCCA 
TGCCCACAGC TGTGCCTGCT CTCATCCTGG TCCTCCTGGG GAGCCTGTAT CCACGACAAC 
TGCATGGAGA CGCATGGACG AAAAGGCTTC AAGCAGAGGA GGAGGGATGT GCTGGAGGAA 
GGATCTGGAA CCGAGAACTG TGCTCATCTC CTGGAGTCAA TGCCCTGTGA GGATCCACTG 
TGCTTCCGGT GGCAGGTGCA GATGGAGAGT GCTTGTGTGC CCAGCAATGG CACCTGCGGG 
AATGGAACCA GGACACAAAC TGTGGCTTGC ATCAATTCAC AAGGGCAGCT TGTTCCAGAG 
GAGCTTTGTG AAGAAGAGTC TCCCCCTGCT GAGGTGTCCT GTGTGATGGC CTGTTCTGGT 
GACTGTGTGC TCAGCTCCTG GTCTCACTGG TCCTCCTGCT CTCACAGCTG CTCCAGCAAA 
AACTCTGAAG GCCGTCAGAG CCGCATACGC ACCATATTAG GCTTGCCAGG AGAAGATAAG 
ACGTGTCCAG CTGCTACTGC TCTGGAGGAG TGGAGAACTT GTAATGATCA TCCATGCATC 
ACCTTCTTCT GGGAAGCTTC GCCGTGGGGG CCTTGCATTG AAGACTCCTC TATGAATGTA 
AATGGCAGCG GCTACTGGAA CGGAACGGCC ACCTGTGCAG TCGGGCTGCA GATCCGTAAA 
GTGGCCTGCA TGAAAATGGG CGTTGGGCCG GTTATTCCCA AAAGGTGTCC AGAATCTGCC 
CGTCCAGACA CCATTCAGCC CTGTTTACTG CCCTGTGAGA AAGATTGCAT TGTGACTCCT 
TTCAGCGAAT GGACAGCCTG CCCGACCACA TGCCTGCCAG TGAACAGCAG CGTGGCCTCA 
CAGTCACGAT ACAGGATCAT CATCCAGCGG GCGGCCAATG GAGGTCAAGA GTGTCCCGAT 
ACTTTATTTG AAGAGAGGGA ATGTGAAGCT CTTCTGCTTT GTCCCACTTA CAGATGGAAG 
ACACACAGAT GGCATCCGTG TACTCTGGTC CCAGATTCAG TCCAGCAGGG AATGACCGGA 
GCGAGTGAAT CGTGTGGTAA AGGATTAGAG ATGAGAGGCG TGAGCTGTGT GAATGAGTAT 
GATGAGATGG TGGCCGTGTC CGAGTGTCTC CAGTGGGCCG GGCCGATGCC GTCTCGCTTC 
AGGAGCTGTT GGGTGGCATG TAAAGATGAC TGTGCCTTTA GTTCCTGGTC GAAATTTTCT 
GAATGCGCTG GATGTGGGAG CAGACGGACA CGCAAACGCA CAATTACAGG TCGTAGCCGA 
AAACTAGACC GATGTCAGCG GGAAGACTTG TTCCCTCTTG TGGAGTCCGA ACCCTGTCCG 
TGTGAGGAGT TTGTTTCTCA GCCATACGGT AATTGGTCGT CTTGTATCCT GTCGGAGACC 
CCAGCACAGG GTTGGAGGGG TCGTCGAGAG GCACGCGACT GTGGTCAGGG ATTCCGCTAT 
CGAGCAATGT CCTGTCTAGA CCACGAATCC CACCTGGTCA GCCCAGAACT CTGCAGCGAA 
TCAGGTCTGG TGGAAGAGGT CTGCTACATA CCCTGCCCTC TGGACTGCAA GCTGAGCGAC 
TGGTCACCGT GGTCAGCCTG CAGCGCCTCC TGTGGCAGTG GACTGAAAAT CAGGTCCAAA 
TGGCTACGAG AGAAGCCTTT CAACGGGGGG AGGCCGTGTC CAAAACTAGA CCTCAAGAAT 
CAGGTGTATG AGGCAGTTCC CTGTCGAAGT GAGTGTGATC AGTACGAGTG GCTAACAGAC 
CCATGGTCAG TCTGTACCAT CAACACAGTG GATGAGCTGC CAGCCTGTGG AGAGGGAGTC 
CAGAGCCGCA AGATCTGGTG TGTGAGTCGT GGCAGTGAGG ACCTGGTGGC CAGTGTGAAT 
GAGACCCTGT GTGACCAGGA TGAAATTCCT CCTCAAGCGC AGACCTGCGT CCTGCCGTGT 
CCTGACGACT GCGTGATGTC CCAGTGGAGT CAGTGGAGCA CCTGTGCCAT ACCGTGTGAC 
GAAAACAGCT CGAGGACTAG GACAAGGCAC GTGCTGCGCC CCCCGGTGGC TGACAGAGTG 
TGTCCAGAAA ATAATCAGAC GGAGCCCTGC ATCCTCAACA GCACCTGCTT CAATTACCAC 
TACAATGTTT CCAGCTGGAG CACCTGTCAG CTGAGCGAAC ATGCCATCTG CGGCCAGGGC 
ACGAGGACAC GACTCTTAGA TTGCATCCGC AGCGATGGCA AAGTGGTGGA TTTGAGCGTG 
TGCGAGGAGT TGGGCCTGCT TCAGCTGTGG AGACTGACCA TGAAATGTGT GGTGGACTGT 
CCCTTTAACT GCATCCTGTC TGACTGGACG TCCTGGACGG AGTGTTCACA CACCTGTGGA 
AGTAAAGGTG TTAAAACTCG CTTTCGCACT GTGCTCCAGC CGGCTCATGA GGAGGGACGT 
CCCTGCGCCT CCCAGCTGTC CCAAACCAAG CCTTGTCCCA TCAGCCCCTG CTACACGTGG 
CTACTGAGCG ACTGGTCTCC CTGCAGAGTC CAGGGAGCTG AATGTGGAGA GGGCACGAGG 
GAGAGGAACC TGACGTGTGT ACAGCACTGG TCAGACTGGC CGGACAGTCC AAACCCTTCT 
GAAGCCCTCG AGGACGATAA GTGTGGAGAC CGACTCCGAA AGCAGAGTGA GCAGGAGCTG 
CAGCAGCCGT GCTTTGTTCC CTGCCCAGGA GACTGTCACC TGACGGAGTG GTCATTGTGG 
AGCTCCTGTC AGCTCACGTG TTTGGAGGGT CGCAGTTTCG AGACAGTGGG GCGTCAGGCA 
CGTTCACAAG CTGTTGTCAT TCAGGTGCTG GACAATCAGG ACAGCTGCCC TCAGCAGGTT 
TTCGAGACCA GGCCGTGCAA AGGAGGGAAA TGTCACAGTT ACGAGTGGAG GACCAGTGCC 
TGGAATGACA ACGAGAGATC AGTGTGGTGC CAGCGGTCTG ACGGGGTCAA TGTGACAGGG 
GGCTGCTTTC CACAGAACCG GCCAACCACT GTGCGCCACT GTCACCCTCC CTGCACTAAA 
CCCTTCTCCC ACTGCAAGCA GAGTGGTGTG TGTGGCTGTG AGAGAGGCTA CACTGAAGTG 
ATGACCACAC ATGGCTTTTT GGACTACTGC ACCAAAACCC CAGGCACCGA CAACAAGAAG 
GCCGATGTGA AGACCAGCTC AGGAAGATTA AGACCAGGCA CGACTCAAAT CCAGGACTTC 
TTCGGCGAGT GGTCCCTGCA GCCTCTCGGC CCAGATGGAA GAATAAAGCT GTGGGTCTAC 
GGGTTAACAG CTGGAGGATT CATTGTCATA TTATTCACAA TTGCTGTGGC CTTTCTGTTT 
TGCAAACCAT CCAAACAGAG TAAAAGTGCA TCTCCACCAC AGAAGCCCTT GACTCTGGCC 
TATGATGGCG ATGTGGACAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

thsd7ba ( XM_009304519.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302794.1 Danio rerio thrombospondin, type I, domain containing 7Ba (thsd7ba), transcript variant X2, mRNA.

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CloneID	ODa44838
Clone ID Related Accession (Same CDS sequence)	XM_009304519.2 , XM_009304519.3
Accession Version	XM_009304519.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4884bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	thrombospondin type-1 domain-containing protein 7B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304519.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAATGG GCTGGACTAT GATTCTAAAA GATGACTCTG GTGTAACGGG CTGGACATGG 
CGGGTTGGGA ACTACCTGCT GCTCTCAATG TCTCTCATCG GAATGCATGT GAAGTCAGAC 
CTGGAATTTC CAAAGGACAG GCACTATACA TGGAAAACGG GTCATTGGGG CAGATGCATA 
GGGGAAGAAT GTGGTACCGG CGGGGTACAA TCCAGGACAG TGTGGTGCAT CCACACAGAA 
GGCTGGACCA CACACCACTC CAACTGTCGG CATTCAGACA AACCCGAGAG CCAGAGGCCT 
TGCTTTAAAG TATGTGAGTG GCACCAGGAC CTCTTTGAAT GGGAAGTGTC AGAATGGGGC 
CCTTGTGTTC TAGCCCCCCT CCTGTCCAAC GAGCTCAAAC TGCGGACCGC ACAGTGCATT 
ACAGCCCAAC ACGGCATCCA GAGACGGAAG GTCCATTGTG TGCGCACTTC CAACCGCACA 
TCGGTTACAG AAAGGATATG CGATTTCTTT TCACCACGAC CAGCTTTCGA GCAGGCCTGC 
TTGATCCCCT GCCCTCATGA CTGCATTGTG TCCGATTTCT CCTCTTGGTC TGGCTGCAGC 
AGAACATGCG GTGCGGGTCT TCAACATCGA ACCCGACATG TCCTAGCAGC GCCAATGTAC 
GGAGGCGCCA ACTGCCCCAA CCTCACCCAA ACAAGGACTT GCTCAAACCT TTTCCCCTGT 
CCTCTTGGGG AAAGCGAGCA TCACTACAGC CTTAAAGTCG GCCTCTGGAG CGAATGCAGG 
CAGCCACAAC ACAAAGGGCT TTGGATGAGC GGAAGGACCG TGCTAGACTT CAGCACAGGA 
GATACCGAAA GAAACACAGT GAAACGACAT ATTCAGAGCT CCCAGCACCA CCATCACCAC 
CTCCACCAAC ACCACCACCA GCACAGTCTC AAGGGTTTAG ATGTGGAGAT CGGCTATCAA 
ACACGGCAAG TTCGTTGCAT GCGCAGCGAT GGAAAAAACG CCATGCTGAG CCTTTGTACC 
CTTGACAACG GCCCTGGCAC TTTCCAGTCT TGTGTTATGC CCAGAGACTG CGAGACCTCC 
GATTGGTCCG TTTGGAGTCC ATGTTCAAAG ACATGCAGAG CATCAGATAT GTCGCCTGGT 
TTTCGGGTCA GGACTCGAAG CATAACGCAC ACTCCCATTG GTCAAGGCAA AGGATGCCCT 
GCTCTTGAAG AAAAGGAGGC CTGCAACATC ATTGGAGATC TGCTTCCAAA CTGCCCAAGG 
TACGTGTGGA AGAGCACCGA TTGGGGGGAC TGTCAAGTGG CCCCTCTTCT TAGCCAGCAG 
GACAGAAGGC TTGACAATGT TAGCGTTCTC TGTGGTGGAG GGATTCAGAC CAGGCAGCTG 
TACTGCGTCC AGGTCCCAGA CAATTCCACC CCTCATCATA GAAAAGAGGT GTCTAGGCCT 
GTAAATGTGC ATCTGTGCAC AGGAGATCCG CCTTCTGCAG TGCAGCCCTG CTCCATTCCA 
TGCCCACAGC TGTGCCTGCT CTCATCCTGG TCCTCCTGGG GAGCCTGTAT CCACGACAAC 
TGCATGGAGA CGCATGGACG AAAAGGCTTC AAGCAGAGGA GGAGGGATGT GCTGGAGGAA 
GGATCTGGAA CCGAGAACTG TGCTCATCTC CTGGAGTCAA TGCCCTGTGA GGATCCACTG 
TGCTTCCGGT GGCAGGTGCA GATGGAGAGT GCTTGTGTGC CCAGCAATGG CACCTGCGGG 
AATGGAACCA GGACACAAAC TGTGGCTTGC ATCAATTCAC AAGGGCAGCT TGTTCCAGAG 
GAGCTTTGTG AAGAAGAGTC TCCCCCTGCT GAGGTGTCCT GTGTGATGGC CTGTTCTGGT 
GACTGTGTGC TCAGCTCCTG GTCTCACTGG TCCTCCTGCT CTCACAGCTG CTCCAGCAAA 
AACTCTGAAG GCCGTCAGAG CCGCATACGC ACCATATTAG GCTTGCCAGG AGAAGATAAG 
ACGTGTCCAG CTGCTACTGC TCTGGAGGAG TGGAGAACTT GTAATGATCA TCCATGCATC 
ACCTTCTTCT GGGAAGCTTC GCCGTGGGGG CCTTGCATTG AAGACTCCTC TATGAATGTA 
AATGGCAGCG GCTACTGGAA CGGAACGGCC ACCTGTGCAG TCGGGCTGCA GATCCGTAAA 
GTGGCCTGCA TGAAAATGGG CGTTGGGCCG GTTATTCCCA AAAGGTGTCC AGAATCTGCC 
CGTCCAGACA CCATTCAGCC CTGTTTACTG CCCTGTGAGA AAGATTGCAT TGTGACTCCT 
TTCAGCGAAT GGACAGCCTG CCCGACCACA TGCCTGCCAG TGAACAGCAG CGTGGCCTCA 
CAGTCACGAT ACAGGATCAT CATCCAGCGG GCGGCCAATG GAGGTCAAGA GTGTCCCGAT 
ACTTTATTTG AAGAGAGGGA ATGTGAAGCT CTTCTGCTTT GTCCCACTTA CAGATGGAAG 
ACACACAGAT GGCATCCGTG TACTCTGGTC CCAGATTCAG TCCAGCAGGG AATGACCGGA 
GCGAGTGAAT CGTGTGGTAA AGGATTAGAG ATGAGAGGCG TGAGCTGTGT GAATGAGTAT 
GATGAGATGG TGGCCGTGTC CGAGTGTCTC CAGTGGGCCG GGCCGATGCC GTCTCGCTTC 
AGGAGCTGTT GGGTGGCATG TAAAGATGAC TGTGCCTTTA GTTCCTGGTC GAAATTTTCT 
GAATGCGCTG GATGTGGGAG CAGACGGACA CGCAAACGCA CAATTACAGG TCGTAGCCGA 
AAACTAGACC GATGTCAGCG GGAAGACTTG TTCCCTCTTG TGGAGTCCGA ACCCTGTCCG 
TGTGAGGAGT TTGTTTCTCA GCCATACGGT AATTGGTCGT CTTGTATCCT GTCGGAGACC 
CCAGCACAGG GTTGGAGGGG TCGTCGAGAG GCACGCGACT GTGGTCAGGG ATTCCGCTAT 
CGAGCAATGT CCTGTCTAGA CCACGAATCC CACCTGGTCA GCCCAGAACT CTGCAGCGAA 
TCAGGTCTGG TGGAAGAGGT CTGCTACATA CCCTGCCCTC TGGACTGCAA GCTGAGCGAC 
TGGTCACCGT GGTCAGCCTG CAGCGCCTCC TGTGGCAGTG GACTGAAAAT CAGGTCCAAA 
TGGCTACGAG AGAAGCCTTT CAACGGGGGG AGGCCGTGTC CAAAACTAGA CCTCAAGAAT 
CAGGTGTATG AGGCAGTTCC CTGTCGAAGT GAGTGTGATC AGTACGAGTG GCTAACAGAC 
CCATGGTCAG TCTGTACCAT CAACACAGTG GATGAGCTGC CAGCCTGTGG AGAGGGAGTC 
CAGAGCCGCA AGATCTGGTG TGTGAGTCGT GGCAGTGAGG ACCTGGTGGC CAGTGTGAAT 
GAGACCCTGT GTGACCAGGA TGAAATTCCT CCTCAAGCGC AGACCTGCGT CCTGCCGTGT 
CCTGACGACT GCGTGATGTC CCAGTGGAGT CAGTGGAGCA CCTGTGCCAT ACCGTGTGAC 
GAAAACAGCT CGAGGACTAG GACAAGGCAC GTGCTGCGCC CCCCGGTGGC TGACAGAGTG 
TGTCCAGAAA ATAATCAGAC GGAGCCCTGC ATCCTCAACA GCACCTGCTT CAATTACCAC 
TACAATGTTT CCAGCTGGAG CACCTGTCAG CTGAGCGAAC ATGCCATCTG CGGCCAGGGC 
ACGAGGACAC GACTCTTAGA TTGCATCCGC AGCGATGGCA AAGTGGTGGA TTTGAGCGTG 
TGCGAGGAGT TGGGCCTGCT TCAGCTGTGG AGACTGACCA TGAAATGTGT GGTGGACTGT 
CCCTTTAACT GCATCCTGTC TGACTGGACG TCCTGGACGG AGTGTTCACA CACCTGTGGA 
AGTAAAGGTG TTAAAACTCG CTTTCGCACT GTGCTCCAGC CGGCTCATGA GGAGGGACGT 
CCCTGCGCCT CCCAGCTGTC CCAAACCAAG CCTTGTCCCA TCAGCCCCTG CTACACGTGG 
CTACTGAGCG ACTGGTCTCC CTGCAGAGTC CAGGGAGCTG AATGTGGAGA GGGCACGAGG 
GAGAGGAACC TGACGTGTGT ACAGCACTGG TCAGACTGGC CGGACAGTCC AAACCCTTCT 
GAAGCCCTCG AGGACGATAA GTGTGGAGAC CGACTCCGAA AGCAGAGTGA GCAGGAGCTG 
CAGCAGCCGT GCTTTGTTCC CTGCCCAGGA GACTGTCACC TGACGGAGTG GTCATTGTGG 
AGCTCCTGTC AGCTCACGTG TTTGGAGGGT CGCAGTTTCG AGACAGTGGG GCGTCAGGCA 
CGTTCACAAG CTGTTGTCAT TCAGGTGCTG GACAATCAGG ACAGCTGCCC TCAGCAGGTT 
TTCGAGACCA GGCCGTGCAA AGGAGGGAAA TGTCACAGTT ACGAGTGGAG GACCAGTGCC 
TGGAATGACA ACGAGAGATC AGTGTGGTGC CAGCGGTCTG ACGGGGTCAA TGTGACAGGG 
GGCTGCTTTC CACAGAACCG GCCAACCACT GTGCGCCACT GTCACCCTCC CTGCACTAAA 
CCCTTCTCCC ACTGCAAGCA GAGTGGTGTG TGTGGCTGTG AGAGAGGCTA CACTGAAGTG 
ATGACCACAC ATGGCTTTTT GGACTACTGC ACCAAAACCC CAGGCACCGA CAACAAGAAG 
GCCGATGTGA AGACCAGCTC AGGAAGATTA AGACCAGGCA CGACTCAAAT CCAGGACTTC 
TTCGGCGAGT GGTCCCTGCA GCCTCTCGGC CCAGATGGAA GAATAAAGCT GTGGGTCTAC 
GGGTTAACAG CTGGAGGATT CATTGTCATA TTATTCACAA TTGCTGTGGC CTTTCTGTTT 
TGCAAACCAT CCAAACAGAG TAAAAGTGCA TCTCCACCAC AGAAGCCCTT GACTCTGGCC 
TATGATGGCG ATGTGGACAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302794.1
CDS	532..5415
Translation	MSMGWTMILKDDSGVTGWTWRVGNYLLLSMSLIGMHVKSDLEFPKDRHYTWKTGHWGRCIGEECGTGGVQSRTVWCIHTEGWTTHHSNCRHSDKPESQRPCFKVCEWHQDLFEWEVSEWGPCVLAPLLSNELKLRTAQCITAQHGIQRRKVHCVRTSNRTSVTERICDFFSPRPAFEQACLIPCPHDCIVSDFSSWSGCSRTCGAGLQHRTRHVLAAPMYGGANCPNLTQTRTCSNLFPCPLGESEHHYSLKVGLWSECRQPQHKGLWMSGRTVLDFSTGDTERNTVKRHIQSSQHHHHHLHQHHHQHSLKGLDVEIGYQTRQVRCMRSDGKNAMLSLCTLDNGPGTFQSCVMPRDCETSDWSVWSPCSKTCRASDMSPGFRVRTRSITHTPIGQGKGCPALEEKEACNIIGDLLPNCPRYVWKSTDWGDCQVAPLLSQQDRRLDNVSVLCGGGIQTRQLYCVQVPDNSTPHHRKEVSRPVNVHLCTGDPPSAVQPCSIPCPQLCLLSSWSSWGACIHDNCMETHGRKGFKQRRRDVLEEGSGTENCAHLLESMPCEDPLCFRWQVQMESACVPSNGTCGNGTRTQTVACINSQGQLVPEELCEEESPPAEVSCVMACSGDCVLSSWSHWSSCSHSCSSKNSEGRQSRIRTILGLPGEDKTCPAATALEEWRTCNDHPCITFFWEASPWGPCIEDSSMNVNGSGYWNGTATCAVGLQIRKVACMKMGVGPVIPKRCPESARPDTIQPCLLPCEKDCIVTPFSEWTACPTTCLPVNSSVASQSRYRIIIQRAANGGQECPDTLFEERECEALLLCPTYRWKTHRWHPCTLVPDSVQQGMTGASESCGKGLEMRGVSCVNEYDEMVAVSECLQWAGPMPSRFRSCWVACKDDCAFSSWSKFSECAGCGSRRTRKRTITGRSRKLDRCQREDLFPLVESEPCPCEEFVSQPYGNWSSCILSETPAQGWRGRREARDCGQGFRYRAMSCLDHESHLVSPELCSESGLVEEVCYIPCPLDCKLSDWSPWSACSASCGSGLKIRSKWLREKPFNGGRPCPKLDLKNQVYEAVPCRSECDQYEWLTDPWSVCTINTVDELPACGEGVQSRKIWCVSRGSEDLVASVNETLCDQDEIPPQAQTCVLPCPDDCVMSQWSQWSTCAIPCDENSSRTRTRHVLRPPVADRVCPENNQTEPCILNSTCFNYHYNVSSWSTCQLSEHAICGQGTRTRLLDCIRSDGKVVDLSVCEELGLLQLWRLTMKCVVDCPFNCILSDWTSWTECSHTCGSKGVKTRFRTVLQPAHEEGRPCASQLSQTKPCPISPCYTWLLSDWSPCRVQGAECGEGTRERNLTCVQHWSDWPDSPNPSEALEDDKCGDRLRKQSEQELQQPCFVPCPGDCHLTEWSLWSSCQLTCLEGRSFETVGRQARSQAVVIQVLDNQDSCPQQVFETRPCKGGKCHSYEWRTSAWNDNERSVWCQRSDGVNVTGGCFPQNRPTTVRHCHPPCTKPFSHCKQSGVCGCERGYTEVMTTHGFLDYCTKTPGTDNKKADVKTSSGRLRPGTTQIQDFFGEWSLQPLGPDGRIKLWVYGLTAGGFIVILFTIAVAFLFCKPSKQSKSASPPQKPLTLAYDGDVDM

Target ORF information:

RefSeq Version	XM_009304519.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio thrombospondin, type I, domain containing 7Ba (thsd7ba), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304519.3

ORF Insert Sequence:

ATGTCAATGG GCTGGACTAT GATTCTAAAA GATGACTCTG GTGTAACGGG CTGGACATGG 
CGGGTTGGGA ACTACCTGCT GCTCTCAATG TCTCTCATCG GAATGCATGT GAAGTCAGAC 
CTGGAATTTC CAAAGGACAG GCACTATACA TGGAAAACGG GTCATTGGGG CAGATGCATA 
GGGGAAGAAT GTGGTACCGG CGGGGTACAA TCCAGGACAG TGTGGTGCAT CCACACAGAA 
GGCTGGACCA CACACCACTC CAACTGTCGG CATTCAGACA AACCCGAGAG CCAGAGGCCT 
TGCTTTAAAG TATGTGAGTG GCACCAGGAC CTCTTTGAAT GGGAAGTGTC AGAATGGGGC 
CCTTGTGTTC TAGCCCCCCT CCTGTCCAAC GAGCTCAAAC TGCGGACCGC ACAGTGCATT 
ACAGCCCAAC ACGGCATCCA GAGACGGAAG GTCCATTGTG TGCGCACTTC CAACCGCACA 
TCGGTTACAG AAAGGATATG CGATTTCTTT TCACCACGAC CAGCTTTCGA GCAGGCCTGC 
TTGATCCCCT GCCCTCATGA CTGCATTGTG TCCGATTTCT CCTCTTGGTC TGGCTGCAGC 
AGAACATGCG GTGCGGGTCT TCAACATCGA ACCCGACATG TCCTAGCAGC GCCAATGTAC 
GGAGGCGCCA ACTGCCCCAA CCTCACCCAA ACAAGGACTT GCTCAAACCT TTTCCCCTGT 
CCTCTTGGGG AAAGCGAGCA TCACTACAGC CTTAAAGTCG GCCTCTGGAG CGAATGCAGG 
CAGCCACAAC ACAAAGGGCT TTGGATGAGC GGAAGGACCG TGCTAGACTT CAGCACAGGA 
GATACCGAAA GAAACACAGT GAAACGACAT ATTCAGAGCT CCCAGCACCA CCATCACCAC 
CTCCACCAAC ACCACCACCA GCACAGTCTC AAGGGTTTAG ATGTGGAGAT CGGCTATCAA 
ACACGGCAAG TTCGTTGCAT GCGCAGCGAT GGAAAAAACG CCATGCTGAG CCTTTGTACC 
CTTGACAACG GCCCTGGCAC TTTCCAGTCT TGTGTTATGC CCAGAGACTG CGAGACCTCC 
GATTGGTCCG TTTGGAGTCC ATGTTCAAAG ACATGCAGAG CATCAGATAT GTCGCCTGGT 
TTTCGGGTCA GGACTCGAAG CATAACGCAC ACTCCCATTG GTCAAGGCAA AGGATGCCCT 
GCTCTTGAAG AAAAGGAGGC CTGCAACATC ATTGGAGATC TGCTTCCAAA CTGCCCAAGG 
TACGTGTGGA AGAGCACCGA TTGGGGGGAC TGTCAAGTGG CCCCTCTTCT TAGCCAGCAG 
GACAGAAGGC TTGACAATGT TAGCGTTCTC TGTGGTGGAG GGATTCAGAC CAGGCAGCTG 
TACTGCGTCC AGGTCCCAGA CAATTCCACC CCTCATCATA GAAAAGAGGT GTCTAGGCCT 
GTAAATGTGC ATCTGTGCAC AGGAGATCCG CCTTCTGCAG TGCAGCCCTG CTCCATTCCA 
TGCCCACAGC TGTGCCTGCT CTCATCCTGG TCCTCCTGGG GAGCCTGTAT CCACGACAAC 
TGCATGGAGA CGCATGGACG AAAAGGCTTC AAGCAGAGGA GGAGGGATGT GCTGGAGGAA 
GGATCTGGAA CCGAGAACTG TGCTCATCTC CTGGAGTCAA TGCCCTGTGA GGATCCACTG 
TGCTTCCGGT GGCAGGTGCA GATGGAGAGT GCTTGTGTGC CCAGCAATGG CACCTGCGGG 
AATGGAACCA GGACACAAAC TGTGGCTTGC ATCAATTCAC AAGGGCAGCT TGTTCCAGAG 
GAGCTTTGTG AAGAAGAGTC TCCCCCTGCT GAGGTGTCCT GTGTGATGGC CTGTTCTGGT 
GACTGTGTGC TCAGCTCCTG GTCTCACTGG TCCTCCTGCT CTCACAGCTG CTCCAGCAAA 
AACTCTGAAG GCCGTCAGAG CCGCATACGC ACCATATTAG GCTTGCCAGG AGAAGATAAG 
ACGTGTCCAG CTGCTACTGC TCTGGAGGAG TGGAGAACTT GTAATGATCA TCCATGCATC 
ACCTTCTTCT GGGAAGCTTC GCCGTGGGGG CCTTGCATTG AAGACTCCTC TATGAATGTA 
AATGGCAGCG GCTACTGGAA CGGAACGGCC ACCTGTGCAG TCGGGCTGCA GATCCGTAAA 
GTGGCCTGCA TGAAAATGGG CGTTGGGCCG GTTATTCCCA AAAGGTGTCC AGAATCTGCC 
CGTCCAGACA CCATTCAGCC CTGTTTACTG CCCTGTGAGA AAGATTGCAT TGTGACTCCT 
TTCAGCGAAT GGACAGCCTG CCCGACCACA TGCCTGCCAG TGAACAGCAG CGTGGCCTCA 
CAGTCACGAT ACAGGATCAT CATCCAGCGG GCGGCCAATG GAGGTCAAGA GTGTCCCGAT 
ACTTTATTTG AAGAGAGGGA ATGTGAAGCT CTTCTGCTTT GTCCCACTTA CAGATGGAAG 
ACACACAGAT GGCATCCGTG TACTCTGGTC CCAGATTCAG TCCAGCAGGG AATGACCGGA 
GCGAGTGAAT CGTGTGGTAA AGGATTAGAG ATGAGAGGCG TGAGCTGTGT GAATGAGTAT 
GATGAGATGG TGGCCGTGTC CGAGTGTCTC CAGTGGGCCG GGCCGATGCC GTCTCGCTTC 
AGGAGCTGTT GGGTGGCATG TAAAGATGAC TGTGCCTTTA GTTCCTGGTC GAAATTTTCT 
GAATGCGCTG GATGTGGGAG CAGACGGACA CGCAAACGCA CAATTACAGG TCGTAGCCGA 
AAACTAGACC GATGTCAGCG GGAAGACTTG TTCCCTCTTG TGGAGTCCGA ACCCTGTCCG 
TGTGAGGAGT TTGTTTCTCA GCCATACGGT AATTGGTCGT CTTGTATCCT GTCGGAGACC 
CCAGCACAGG GTTGGAGGGG TCGTCGAGAG GCACGCGACT GTGGTCAGGG ATTCCGCTAT 
CGAGCAATGT CCTGTCTAGA CCACGAATCC CACCTGGTCA GCCCAGAACT CTGCAGCGAA 
TCAGGTCTGG TGGAAGAGGT CTGCTACATA CCCTGCCCTC TGGACTGCAA GCTGAGCGAC 
TGGTCACCGT GGTCAGCCTG CAGCGCCTCC TGTGGCAGTG GACTGAAAAT CAGGTCCAAA 
TGGCTACGAG AGAAGCCTTT CAACGGGGGG AGGCCGTGTC CAAAACTAGA CCTCAAGAAT 
CAGGTGTATG AGGCAGTTCC CTGTCGAAGT GAGTGTGATC AGTACGAGTG GCTAACAGAC 
CCATGGTCAG TCTGTACCAT CAACACAGTG GATGAGCTGC CAGCCTGTGG AGAGGGAGTC 
CAGAGCCGCA AGATCTGGTG TGTGAGTCGT GGCAGTGAGG ACCTGGTGGC CAGTGTGAAT 
GAGACCCTGT GTGACCAGGA TGAAATTCCT CCTCAAGCGC AGACCTGCGT CCTGCCGTGT 
CCTGACGACT GCGTGATGTC CCAGTGGAGT CAGTGGAGCA CCTGTGCCAT ACCGTGTGAC 
GAAAACAGCT CGAGGACTAG GACAAGGCAC GTGCTGCGCC CCCCGGTGGC TGACAGAGTG 
TGTCCAGAAA ATAATCAGAC GGAGCCCTGC ATCCTCAACA GCACCTGCTT CAATTACCAC 
TACAATGTTT CCAGCTGGAG CACCTGTCAG CTGAGCGAAC ATGCCATCTG CGGCCAGGGC 
ACGAGGACAC GACTCTTAGA TTGCATCCGC AGCGATGGCA AAGTGGTGGA TTTGAGCGTG 
TGCGAGGAGT TGGGCCTGCT TCAGCTGTGG AGACTGACCA TGAAATGTGT GGTGGACTGT 
CCCTTTAACT GCATCCTGTC TGACTGGACG TCCTGGACGG AGTGTTCACA CACCTGTGGA 
AGTAAAGGTG TTAAAACTCG CTTTCGCACT GTGCTCCAGC CGGCTCATGA GGAGGGACGT 
CCCTGCGCCT CCCAGCTGTC CCAAACCAAG CCTTGTCCCA TCAGCCCCTG CTACACGTGG 
CTACTGAGCG ACTGGTCTCC CTGCAGAGTC CAGGGAGCTG AATGTGGAGA GGGCACGAGG 
GAGAGGAACC TGACGTGTGT ACAGCACTGG TCAGACTGGC CGGACAGTCC AAACCCTTCT 
GAAGCCCTCG AGGACGATAA GTGTGGAGAC CGACTCCGAA AGCAGAGTGA GCAGGAGCTG 
CAGCAGCCGT GCTTTGTTCC CTGCCCAGGA GACTGTCACC TGACGGAGTG GTCATTGTGG 
AGCTCCTGTC AGCTCACGTG TTTGGAGGGT CGCAGTTTCG AGACAGTGGG GCGTCAGGCA 
CGTTCACAAG CTGTTGTCAT TCAGGTGCTG GACAATCAGG ACAGCTGCCC TCAGCAGGTT 
TTCGAGACCA GGCCGTGCAA AGGAGGGAAA TGTCACAGTT ACGAGTGGAG GACCAGTGCC 
TGGAATGACA ACGAGAGATC AGTGTGGTGC CAGCGGTCTG ACGGGGTCAA TGTGACAGGG 
GGCTGCTTTC CACAGAACCG GCCAACCACT GTGCGCCACT GTCACCCTCC CTGCACTAAA 
CCCTTCTCCC ACTGCAAGCA GAGTGGTGTG TGTGGCTGTG AGAGAGGCTA CACTGAAGTG 
ATGACCACAC ATGGCTTTTT GGACTACTGC ACCAAAACCC CAGGCACCGA CAACAAGAAG 
GCCGATGTGA AGACCAGCTC AGGAAGATTA AGACCAGGCA CGACTCAAAT CCAGGACTTC 
TTCGGCGAGT GGTCCCTGCA GCCTCTCGGC CCAGATGGAA GAATAAAGCT GTGGGTCTAC 
GGGTTAACAG CTGGAGGATT CATTGTCATA TTATTCACAA TTGCTGTGGC CTTTCTGTTT 
TGCAAACCAT CCAAACAGAG TAAAAGTGCA TCTCCACCAC AGAAGCCCTT GACTCTGGCC 
TATGATGGCG ATGTGGACAT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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thsd7ba cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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