EIF4G3A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	EIF4G3A
Entrez Gene ID	566131
Full Name	eukaryotic translation initiation factor 4 gamma, 3a
Synonyms	eif4g3
General protein information	Preferred Names eukaryotic translation initiation factor 4 gamma, 3a Names eukaryotic translation initiation factor 4 gamma 3 eukaryotic translation initiation factor 4 gamma, 3
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009306089.2	XP_009304364.1	eukaryotic translation initiation factor 4 gamma 3 isoform X1
XM_009306089.3	XP_009304364.1	eukaryotic translation initiation factor 4 gamma 3 isoform X1
XM_689398.8	XP_694490.5	eukaryotic translation initiation factor 4 gamma 3 isoform X3
XM_689398.7	XP_694490.5	eukaryotic translation initiation factor 4 gamma 3 isoform X3
XM_009306090.3	XP_009304365.1	eukaryotic translation initiation factor 4 gamma 3 isoform X2
XM_009306090.2	XP_009304365.1	eukaryotic translation initiation factor 4 gamma 3 isoform X2
XM_017358339.1	XP_017213828.1	eukaryotic translation initiation factor 4 gamma 3 isoform X4

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	eif4g3	XP_694490.5
Danio rerio (zebrafish)	eif4g3b	XP_001920081.2
Pan troglodytes (chimpanzee)	EIF4G3	XP_003307899.2
Bos taurus (cattle)	EIF4G3	XP_005203398.1
	LOC717555	XP_002802261.1
Xenopus tropicalis (tropical clawed frog)	LOC100216088	NP_001135544.1
Canis lupus familiaris (dog)	EIF4G3	XP_005617902.1
Mus musculus (house mouse)	Eif4g3	NP_766291.2
Rattus norvegicus (Norway rat)	Eif4g3	NP_001100163.1
Homo sapiens (human)	EIF4G3	NP_001185730.1
Gallus gallus (chicken)	EIF4G3	XP_004947597.1

Gene Ontology

The following EIF4G3A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF4G3A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa54356	XM_017358339.1 Latest version!	Danio rerio eukaryotic translation initiation factor 4 gamma, 3a (eif4g3a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa17153	XM_689398.8 Latest version!	Danio rerio eukaryotic translation initiation factor 4 gamma, 3a (eif4g3a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa44791	XM_009306089.3 Latest version!	Danio rerio eukaryotic translation initiation factor 4 gamma, 3a (eif4g3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa44792	XM_009306090.3 Latest version!	Danio rerio eukaryotic translation initiation factor 4 gamma, 3a (eif4g3a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa44791	XM_009306089.2	Danio rerio eukaryotic translation initiation factor 4 gamma, 3a (eif4g3a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa17153	XM_689398.7	Danio rerio eukaryotic translation initiation factor 4 gamma, 3a (eif4g3a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa44792	XM_009306090.2	Danio rerio eukaryotic translation initiation factor 4 gamma, 3a (eif4g3a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EIF4G3A ( XM_017358339.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017213828.1 Danio rerio eukaryotic translation initiation factor 4 gamma, 3a (eif4g3a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa54356
Clone ID Related Accession (Same CDS sequence)	XM_017358339.1
Accession Version	XM_017358339.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	eukaryotic translation initiation factor 4 gamma 3 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTTGC CACCGAAAGT GGTTCCGAAA CCGGCCGCTG TCGCTGTTAG TGGACACGTA 
ACCGGACCGG CACCGCCAAC GCAGCTCCGC GCAGCGCTGA CCTCCGTGTC GTTGCCACCG 
GGAGCCCAAA ACGCTCCGCC GTCAGCCGTG CCGCCTACCC AGATTCCTAG AGCAGCGCTA 
TCACTGGATG AACGCATGTT TCCCGCTCAC TCTGGGGTGA CTGCGGTCTA CAGTGTATCC 
AGACACCCTG GTCCTCCATT TCCAGGTCAT GACCTCAGCA AAACCCACCC AAACCTGGCA 
GGGACTCCTC CTGGCCATGC CACGTCCCCG GCCCTCTCTC AGGTATCAGT TCCTGCTGGC 
CCTTCGTACC GCATCCTCAA GCCCTGGGAG ACAGGCGGAG CGCCTCCATA TAACCCAGCT 
CAGAATGCAG GCTCCGCCCC CCTGGTGTAC TCGCCACAGA CGCAGCCAAT GAACGTACAG 
CCACAGACTC GACCGTTTGT GACGGGACCT CGGCCAACAC ACCATCAGTT CATACACAGG 
AGTCAAATGC AGCCAGCGCG GCCTACCCTT CCGACTAACA ACCCCTCCAT TCGGCCGGGC 
TCACAGACCC CCACGGCGAC AGTTTATCCA CCAAACCAAC CCATCATGAT GACCATGACG 
CCCATGCCAT TTGCTACCCA GACACACCAA TATTACATTC CCCAGTATCG TCATAGTGCA 
CCTTACGTGG GACCCCCGCA GCAGTATGCC GTCCAGCCTC CAGGGTCTGG AACTTTCTAC 
CCTGGACCCA GCCCTGCGGA ATATCCCACC CCTTATGCTG CTGGTCCGCC ATATTACACC 
GGGCAGACGG TGTACCCACC CTCACCACCC ATCATAGTGC CTGCACCAAT GCCACCTCCA 
CCAACCAAGC GAGAAAAGAA ACCATCCTCC CAGATCCGGA TCCGAGACCC CAACCAGGGT 
GGAAAGGACA TCACAGAGGA AATCATGTTT GGGAGCCGCA ATCCTACACC TCCTGCCGGA 
CACCCAGCCT CCACCTTAAC CCCACCTGCA GGACGTCCCT CCTCGACTCC GACCCCACCT 
TCAGGACGCC TGTCCTCCAC ACCCACCCCT CCACAGCGGC CCAGCAACTG TCAAACCCCA 
GAACAAACGG CATATGTCAA CCAAAATCAG AGACTCTCAG AGAGCCCTGC GCCAATGGAT 
GGCAAGCCCA GCTTAGCTAT AGACGACAGG CCAAAAATGG AGTCTGGTCC CATTAAGTCT 
ATATCTCCTG GACCAAGACC TTCAGAATCC TGTTTGGAGA AAAGAGAAAT CTCAAGTCTT 
CCATTACTAG TCTCTTCTTC TCCAGAGGTG GATGTTTCTT CCCATCCTAC AAGTGGATGC 
ATCAAACCCA CTGCCGCAGG AGAACCAGAA TTTATCTCTC CTTCAGCCAC TAAGGCTCAG 
ACGTATCAGG TCATCTCCGG TGAGGAATCT GTTCCTGAAG CTTCTCCCAG ATTGTCTGCT 
TCATTGTCCC TCAGAGTGGT AAATGGTGTC AATGAGCCTC AAACTCCCTC AAGCTATGAA 
GAGCCTGAGG TCCAGGAAGC CCTAAAGATG TCGTCATCCT GCGAGATTCA GGGTACATCA 
TTCATGGAGG AGTCTGGTCA GGAAGTTCCA GTGGCCTTGG AGGAGCTCCA GGCAGAGCAC 
CTCCCTTCTC TAGCTGCACA TGTGCCCTTG ATCCCAGGCG TACAGGCCTC TTCAATCACC 
TCCAGCACCA CTTCTGTTCT TGCGCCACCA CCTGGGTTGG CACCATTAGT GCAGTCGGTT 
GTCCTTGAGG TGTCAGAACA GAACAAGACC TCAGACGATG TCCAGCTGAA AGCTCAAGAT 
GCTTCAGAAT CACAAACTCA AAGCAACTCT AGAAAGTTTA CACCAGCCCA AGCTGTCCCT 
GTTGTACCAA AGCCTTGGAA GAAACCAAAC GACGAAGATC TTATGATGGA AACGCTGAAG 
AAAGACAATG AGGAGGAGAC CTCAAAGGAC TGCTCTGCTG GAGATGAGAT GGTCTTGCAG 
TCAAGCTTAG GGACCTACAG CAGCCCCTTT ACAGTGCCTG AGCACCAGGA GAAGCACTCT 
GAAGAAAACG GTGAGACCGA GACAGAGCCT TTCAGGAACG GGGTTGAGAC GGAGAGCACG 
GACAGTGGTG TGACGCTCGG GGACAGTAGG GGCTCCATGT GTTCCCCAAC TCCACTCATT 
CAGGAAGGTC TTGTGCAATC TAATGGAAAG AGACAGTATG ACCGGGACTT CCTGTTGGGC 
TTTCAGTTTA TGCCGGCCTG TGTGCAGAAG CCCGAGGGAC TGCCTCCAAT CAGTGATGTG 
GTGCTAGACA AGATGAACCA AAACAAGCTG CCTCTGAGGT CTGTGGACCC CAGGTTGATT 
TCCAGAGGGA CGCCTCAGGA TTTCACTCCT GCTTTTGCTG ATTTCAGCAG GCAGATGGCT 
GGAGGACGCG GAGCCCCGCT TTTAAATGTG CGACGACTCC CACCACGCAA GATCATCGTA 
AACCTGTCTG TCAGTGATGA TGTTCAGCTC AAGAAGGCAG AAAATGCTTG GAAACCTGGG 
ATGAAGAGAG AGGGTGTCGC AGAGGACCCA GAAACCATCA GAACCCAGGA GTTGTTCCGG 
AAAGTTCGTA GCATTTTAAA CAAGCTCACG CCGCAGATGT TTCATCAGCT GATGAAGCAA 
GTGACGGATC TTACTATCAA CACAGAGGAG AGGCTTAAAG GAGTCATCGA TCTTGTGTTT 
GAGAAGGCCA TCGATGAGCC CAGCTTCTCT GTGGCCTACG CCAACATGTG TCGCTGCCTG 
GCCACGCTCA AAGTGCCCAC AGCAGACAAA CCTAATACAA CGGTGAACTT CCGGAAGCTC 
CTGTTGAATC GCTGTCAAAA GGAATTCGAG AGGGACAAAG TAGATGACGT TGTCCTGGAG 
AGAAAACAAA AGGAGATTGA CTCTGCCACA TCACCCACAG AAAAGGAAAG ACTGCAGGAG 
GAGCTGGAGG AGGCGAAGGA CAAGGCCAGA CGTCGCTCCA CTGGAAATAT CAAATTCATC 
GGTGAGCTGT TCAAGCTCAA GATGTTGACA GAGCCCATCA TGCATGACTG CGTGGTGAAA 
CTGCTCAAAA ACCACGATGA TGAGAGCTTA GAGTGCCTCT GCAGACTCCT CACCACCATC 
GGCAAAGACC TGGACTTCGA GAAGGCCAAG CCCCGAATGG ACCAGTACTT CAACCAGATG 
GAGAAAATTG TCAAAGAGCG CAAGACCTCG TCTCGGATAC GGTTCATGCT GCAGGATGTG 
ATAGATCTGA GACTGCATAA CTGGGTTTCT CGGAGAGCTG ACCAAGGCCC TAAAACCATC 
GAGCAGATCC ATAAAGAGGC CAAGCTGGAG GAGCAGGAGG AGCAGAGGAA AGTCCACCAG 
CAGCTTTTGT CTAAAGACAT GAAGAGGAGG CCAGATCAGA GAGAACAGCG TTCAATGAGA 
GAGGAGACTT GGAACACTGT GCCCACTACC AAAAACAGCA GGACTATCGA CCCTACAAAG 
ATTCCAAAGA TCTCCAAGCC GCAAATCGAT GAAAAGATTC AGCTGGGTCC GCGAGCTCAG 
ATCAACTGGG TGAAAGGCAG CAGCGGCGGA GCAAAAGCCA GCGACTCTGA GTTGACCCGC 
TCTGCCGGAA ACACTCTGAA CCGCTATTCA GCACTGCAGT CATCCACGTC TCAATCCAGT 
TCAACTCCGG CCCAAACCTC TGAATCTGAG TCCAGACGCT CGCTGGGAAG TCGAGGCAGT 
ATGGGTCGCG AGCGCAGTGA AAAGCCTGTG AGTTTGGGGC CGTCACGACC GGGTCCTTTT 
GGCAGAGGTA GCAGCGCTAA GGAGCTAACA GATAACATGG TCCCAGAGGA AACACGGAGA 
GAAGGACAAG ACTCACACCA CACATCTCGC AAAGCCAGCA TCTCAGAGGA GAAATGTGAG 
ATGGAGAACA GCAGATCCGC AGAGTCAGTG AAATCAGAGA CGCTGCTGGT CAGCGGTGTT 
TCAGAGAAGC CTGTCCTGTC TGAAGAGGAG ATCGAGCGGA AGTCCAAAGC TATCATTGAT 
GAATTTCTGC ACATTAATGA CTACAAGGAG GCAGTGCAGT GTGTGCTGGA GATAGAGCAG 
CCCTCTATGC TGTGTGTGTT TGTGCGCATG GGTCTGGAGA GCACACTGGA GCGCAGTCAG 
AAAGCCAGAG AGCACATGGG ACTGCTCTAC TATCAGCTTA TACAAAAGGG CATCTTACCC 
CATTCTCAAC TCTACAAAGG GTTTTCAGAG ATGTTGGAGC AGGCTGATGA CATGGCCATA 
GACATTCCCT TCATTTGGCT TTATCTGGCT GAGCTGCTCA GTCCTCTGTT GAAAGAGGGA 
GGAATCAACA TGAGAGAGCT GTTCAGTGAG TTAAGTAAAC CGCTACTCCC TGTGGGAAGA 
GCTGCCATCT TATTGTCTGA AATACTGCAC CTACTATGCA AACAAATGAG CCATAGGAAA 
GTTGGAGACT TGTGGAGAGA GTCTGGGCTG AACTGGGCCG ACTTCCTGCC CGAGGGAGAA 
CATCTTCCTG CCTTCATCTC ACAACATAAG TTAGAATTCA CAGTGTCGGA CATCTCTCCT 
GTCTCTCCGG CCTCAGCCAA ACCCAGCCTC AGTCCTGAGG AGCTCTTTAA ACAACTGGAG 
CAGCTCCTGC TAGAGGACAT GTCCAGCGAT GAACAGATTT TTGACTGGAT CGAGGAGAGC 
GACCATCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213828.1
CDS	493..5244
Translation	MSLPPKVVPKPAAVAVSGHVTGPAPPTQLRAALTSVSLPPGAQNAPPSAVPPTQIPRAALSLDERMFPAHSGVTAVYSVSRHPGPPFPGHDLSKTHPNLAGTPPGHATSPALSQVSVPAGPSYRILKPWETGGAPPYNPAQNAGSAPLVYSPQTQPMNVQPQTRPFVTGPRPTHHQFIHRSQMQPARPTLPTNNPSIRPGSQTPTATVYPPNQPIMMTMTPMPFATQTHQYYIPQYRHSAPYVGPPQQYAVQPPGSGTFYPGPSPAEYPTPYAAGPPYYTGQTVYPPSPPIIVPAPMPPPPTKREKKPSSQIRIRDPNQGGKDITEEIMFGSRNPTPPAGHPASTLTPPAGRPSSTPTPPSGRLSSTPTPPQRPSNCQTPEQTAYVNQNQRLSESPAPMDGKPSLAIDDRPKMESGPIKSISPGPRPSESCLEKREISSLPLLVSSSPEVDVSSHPTSGCIKPTAAGEPEFISPSATKAQTYQVISGEESVPEASPRLSASLSLRVVNGVNEPQTPSSYEEPEVQEALKMSSSCEIQGTSFMEESGQEVPVALEELQAEHLPSLAAHVPLIPGVQASSITSSTTSVLAPPPGLAPLVQSVVLEVSEQNKTSDDVQLKAQDASESQTQSNSRKFTPAQAVPVVPKPWKKPNDEDLMMETLKKDNEEETSKDCSAGDEMVLQSSLGTYSSPFTVPEHQEKHSEENGETETEPFRNGVETESTDSGVTLGDSRGSMCSPTPLIQEGLVQSNGKRQYDRDFLLGFQFMPACVQKPEGLPPISDVVLDKMNQNKLPLRSVDPRLISRGTPQDFTPAFADFSRQMAGGRGAPLLNVRRLPPRKIIVNLSVSDDVQLKKAENAWKPGMKREGVAEDPETIRTQELFRKVRSILNKLTPQMFHQLMKQVTDLTINTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLATLKVPTADKPNTTVNFRKLLLNRCQKEFERDKVDDVVLERKQKEIDSATSPTEKERLQEELEEAKDKARRRSTGNIKFIGELFKLKMLTEPIMHDCVVKLLKNHDDESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLHNWVSRRADQGPKTIEQIHKEAKLEEQEEQRKVHQQLLSKDMKRRPDQREQRSMREETWNTVPTTKNSRTIDPTKIPKISKPQIDEKIQLGPRAQINWVKGSSGGAKASDSELTRSAGNTLNRYSALQSSTSQSSSTPAQTSESESRRSLGSRGSMGRERSEKPVSLGPSRPGPFGRGSSAKELTDNMVPEETRREGQDSHHTSRKASISEEKCEMENSRSAESVKSETLLVSGVSEKPVLSEEEIERKSKAIIDEFLHINDYKEAVQCVLEIEQPSMLCVFVRMGLESTLERSQKAREHMGLLYYQLIQKGILPHSQLYKGFSEMLEQADDMAIDIPFIWLYLAELLSPLLKEGGINMRELFSELSKPLLPVGRAAILLSEILHLLCKQMSHRKVGDLWRESGLNWADFLPEGEHLPAFISQHKLEFTVSDISPVSPASAKPSLSPEELFKQLEQLLLEDMSSDEQIFDWIEESDHL

Target ORF information:

RefSeq Version	XM_017358339.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio eukaryotic translation initiation factor 4 gamma, 3a (eif4g3a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358339.1

ORF Insert Sequence:

ATGAGTTTGC CACCGAAAGT GGTTCCGAAA CCGGCCGCTG TCGCTGTTAG TGGACACGTA 
ACCGGACCGG CACCGCCAAC GCAGCTCCGC GCAGCGCTGA CCTCCGTGTC GTTGCCACCG 
GGAGCCCAAA ACGCTCCGCC GTCAGCCGTG CCGCCTACCC AGATTCCTAG AGCAGCGCTA 
TCACTGGATG AACGCATGTT TCCCGCTCAC TCTGGGGTGA CTGCGGTCTA CAGTGTATCC 
AGACACCCTG GTCCTCCATT TCCAGGTCAT GACCTCAGCA AAACCCACCC AAACCTGGCA 
GGGACTCCTC CTGGCCATGC CACGTCCCCG GCCCTCTCTC AGGTATCAGT TCCTGCTGGC 
CCTTCGTACC GCATCCTCAA GCCCTGGGAG ACAGGCGGAG CGCCTCCATA TAACCCAGCT 
CAGAATGCAG GCTCCGCCCC CCTGGTGTAC TCGCCACAGA CGCAGCCAAT GAACGTACAG 
CCACAGACTC GACCGTTTGT GACGGGACCT CGGCCAACAC ACCATCAGTT CATACACAGG 
AGTCAAATGC AGCCAGCGCG GCCTACCCTT CCGACTAACA ACCCCTCCAT TCGGCCGGGC 
TCACAGACCC CCACGGCGAC AGTTTATCCA CCAAACCAAC CCATCATGAT GACCATGACG 
CCCATGCCAT TTGCTACCCA GACACACCAA TATTACATTC CCCAGTATCG TCATAGTGCA 
CCTTACGTGG GACCCCCGCA GCAGTATGCC GTCCAGCCTC CAGGGTCTGG AACTTTCTAC 
CCTGGACCCA GCCCTGCGGA ATATCCCACC CCTTATGCTG CTGGTCCGCC ATATTACACC 
GGGCAGACGG TGTACCCACC CTCACCACCC ATCATAGTGC CTGCACCAAT GCCACCTCCA 
CCAACCAAGC GAGAAAAGAA ACCATCCTCC CAGATCCGGA TCCGAGACCC CAACCAGGGT 
GGAAAGGACA TCACAGAGGA AATCATGTTT GGGAGCCGCA ATCCTACACC TCCTGCCGGA 
CACCCAGCCT CCACCTTAAC CCCACCTGCA GGACGTCCCT CCTCGACTCC GACCCCACCT 
TCAGGACGCC TGTCCTCCAC ACCCACCCCT CCACAGCGGC CCAGCAACTG TCAAACCCCA 
GAACAAACGG CATATGTCAA CCAAAATCAG AGACTCTCAG AGAGCCCTGC GCCAATGGAT 
GGCAAGCCCA GCTTAGCTAT AGACGACAGG CCAAAAATGG AGTCTGGTCC CATTAAGTCT 
ATATCTCCTG GACCAAGACC TTCAGAATCC TGTTTGGAGA AAAGAGAAAT CTCAAGTCTT 
CCATTACTAG TCTCTTCTTC TCCAGAGGTG GATGTTTCTT CCCATCCTAC AAGTGGATGC 
ATCAAACCCA CTGCCGCAGG AGAACCAGAA TTTATCTCTC CTTCAGCCAC TAAGGCTCAG 
ACGTATCAGG TCATCTCCGG TGAGGAATCT GTTCCTGAAG CTTCTCCCAG ATTGTCTGCT 
TCATTGTCCC TCAGAGTGGT AAATGGTGTC AATGAGCCTC AAACTCCCTC AAGCTATGAA 
GAGCCTGAGG TCCAGGAAGC CCTAAAGATG TCGTCATCCT GCGAGATTCA GGGTACATCA 
TTCATGGAGG AGTCTGGTCA GGAAGTTCCA GTGGCCTTGG AGGAGCTCCA GGCAGAGCAC 
CTCCCTTCTC TAGCTGCACA TGTGCCCTTG ATCCCAGGCG TACAGGCCTC TTCAATCACC 
TCCAGCACCA CTTCTGTTCT TGCGCCACCA CCTGGGTTGG CACCATTAGT GCAGTCGGTT 
GTCCTTGAGG TGTCAGAACA GAACAAGACC TCAGACGATG TCCAGCTGAA AGCTCAAGAT 
GCTTCAGAAT CACAAACTCA AAGCAACTCT AGAAAGTTTA CACCAGCCCA AGCTGTCCCT 
GTTGTACCAA AGCCTTGGAA GAAACCAAAC GACGAAGATC TTATGATGGA AACGCTGAAG 
AAAGACAATG AGGAGGAGAC CTCAAAGGAC TGCTCTGCTG GAGATGAGAT GGTCTTGCAG 
TCAAGCTTAG GGACCTACAG CAGCCCCTTT ACAGTGCCTG AGCACCAGGA GAAGCACTCT 
GAAGAAAACG GTGAGACCGA GACAGAGCCT TTCAGGAACG GGGTTGAGAC GGAGAGCACG 
GACAGTGGTG TGACGCTCGG GGACAGTAGG GGCTCCATGT GTTCCCCAAC TCCACTCATT 
CAGGAAGGTC TTGTGCAATC TAATGGAAAG AGACAGTATG ACCGGGACTT CCTGTTGGGC 
TTTCAGTTTA TGCCGGCCTG TGTGCAGAAG CCCGAGGGAC TGCCTCCAAT CAGTGATGTG 
GTGCTAGACA AGATGAACCA AAACAAGCTG CCTCTGAGGT CTGTGGACCC CAGGTTGATT 
TCCAGAGGGA CGCCTCAGGA TTTCACTCCT GCTTTTGCTG ATTTCAGCAG GCAGATGGCT 
GGAGGACGCG GAGCCCCGCT TTTAAATGTG CGACGACTCC CACCACGCAA GATCATCGTA 
AACCTGTCTG TCAGTGATGA TGTTCAGCTC AAGAAGGCAG AAAATGCTTG GAAACCTGGG 
ATGAAGAGAG AGGGTGTCGC AGAGGACCCA GAAACCATCA GAACCCAGGA GTTGTTCCGG 
AAAGTTCGTA GCATTTTAAA CAAGCTCACG CCGCAGATGT TTCATCAGCT GATGAAGCAA 
GTGACGGATC TTACTATCAA CACAGAGGAG AGGCTTAAAG GAGTCATCGA TCTTGTGTTT 
GAGAAGGCCA TCGATGAGCC CAGCTTCTCT GTGGCCTACG CCAACATGTG TCGCTGCCTG 
GCCACGCTCA AAGTGCCCAC AGCAGACAAA CCTAATACAA CGGTGAACTT CCGGAAGCTC 
CTGTTGAATC GCTGTCAAAA GGAATTCGAG AGGGACAAAG TAGATGACGT TGTCCTGGAG 
AGAAAACAAA AGGAGATTGA CTCTGCCACA TCACCCACAG AAAAGGAAAG ACTGCAGGAG 
GAGCTGGAGG AGGCGAAGGA CAAGGCCAGA CGTCGCTCCA CTGGAAATAT CAAATTCATC 
GGTGAGCTGT TCAAGCTCAA GATGTTGACA GAGCCCATCA TGCATGACTG CGTGGTGAAA 
CTGCTCAAAA ACCACGATGA TGAGAGCTTA GAGTGCCTCT GCAGACTCCT CACCACCATC 
GGCAAAGACC TGGACTTCGA GAAGGCCAAG CCCCGAATGG ACCAGTACTT CAACCAGATG 
GAGAAAATTG TCAAAGAGCG CAAGACCTCG TCTCGGATAC GGTTCATGCT GCAGGATGTG 
ATAGATCTGA GACTGCATAA CTGGGTTTCT CGGAGAGCTG ACCAAGGCCC TAAAACCATC 
GAGCAGATCC ATAAAGAGGC CAAGCTGGAG GAGCAGGAGG AGCAGAGGAA AGTCCACCAG 
CAGCTTTTGT CTAAAGACAT GAAGAGGAGG CCAGATCAGA GAGAACAGCG TTCAATGAGA 
GAGGAGACTT GGAACACTGT GCCCACTACC AAAAACAGCA GGACTATCGA CCCTACAAAG 
ATTCCAAAGA TCTCCAAGCC GCAAATCGAT GAAAAGATTC AGCTGGGTCC GCGAGCTCAG 
ATCAACTGGG TGAAAGGCAG CAGCGGCGGA GCAAAAGCCA GCGACTCTGA GTTGACCCGC 
TCTGCCGGAA ACACTCTGAA CCGCTATTCA GCACTGCAGT CATCCACGTC TCAATCCAGT 
TCAACTCCGG CCCAAACCTC TGAATCTGAG TCCAGACGCT CGCTGGGAAG TCGAGGCAGT 
ATGGGTCGCG AGCGCAGTGA AAAGCCTGTG AGTTTGGGGC CGTCACGACC GGGTCCTTTT 
GGCAGAGGTA GCAGCGCTAA GGAGCTAACA GATAACATGG TCCCAGAGGA AACACGGAGA 
GAAGGACAAG ACTCACACCA CACATCTCGC AAAGCCAGCA TCTCAGAGGA GAAATGTGAG 
ATGGAGAACA GCAGATCCGC AGAGTCAGTG AAATCAGAGA CGCTGCTGGT CAGCGGTGTT 
TCAGAGAAGC CTGTCCTGTC TGAAGAGGAG ATCGAGCGGA AGTCCAAAGC TATCATTGAT 
GAATTTCTGC ACATTAATGA CTACAAGGAG GCAGTGCAGT GTGTGCTGGA GATAGAGCAG 
CCCTCTATGC TGTGTGTGTT TGTGCGCATG GGTCTGGAGA GCACACTGGA GCGCAGTCAG 
AAAGCCAGAG AGCACATGGG ACTGCTCTAC TATCAGCTTA TACAAAAGGG CATCTTACCC 
CATTCTCAAC TCTACAAAGG GTTTTCAGAG ATGTTGGAGC AGGCTGATGA CATGGCCATA 
GACATTCCCT TCATTTGGCT TTATCTGGCT GAGCTGCTCA GTCCTCTGTT GAAAGAGGGA 
GGAATCAACA TGAGAGAGCT GTTCAGTGAG TTAAGTAAAC CGCTACTCCC TGTGGGAAGA 
GCTGCCATCT TATTGTCTGA AATACTGCAC CTACTATGCA AACAAATGAG CCATAGGAAA 
GTTGGAGACT TGTGGAGAGA GTCTGGGCTG AACTGGGCCG ACTTCCTGCC CGAGGGAGAA 
CATCTTCCTG CCTTCATCTC ACAACATAAG TTAGAATTCA CAGTGTCGGA CATCTCTCCT 
GTCTCTCCGG CCTCAGCCAA ACCCAGCCTC AGTCCTGAGG AGCTCTTTAA ACAACTGGAG 
CAGCTCCTGC TAGAGGACAT GTCCAGCGAT GAACAGATTT TTGACTGGAT CGAGGAGAGC 
GACCATCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G3A ( XM_689398.8 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_694490.5 Danio rerio eukaryotic translation initiation factor 4 gamma, 3a (eif4g3a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa17153
Clone ID Related Accession (Same CDS sequence)	XM_689398.8 , XM_689398.7
Accession Version	XM_689398.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	eukaryotic translation initiation factor 4 gamma 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_689398.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTTGC CACCGAAAGT GGTTCCGAAA CCGGCCGCTG TCGCTGTTAG TGGACACGTA 
ACCGGACCGG CACCGCCAAC GCAGCTCCGC GCAGCGCTGA CCTCCGTGTC GTTGCCACCG 
GGAGCCCAAA ACGCTCCGCC GTCAGCCGTG CCGCCTACCC AGATTCCTAG AGCAGCGCTA 
TCACTGGATG AACGCATGTT TCCCGCTCAC TCTGGGGTGA CTGCGGTCTA CAGTGTATCC 
AGACACCCTG GTCCTCCATT TCCAGGTCAT GACCTCAGCA AAACCCACCC AAACCTGGCA 
GGGACTCCTC CTGGCCATGC CACGTCCCCG GCCCTCTCTC AGGTATCAGT TCCTGCTGGC 
CCTTCGTACC GCATCCTCAA GCCCTGGGAG ACAGGCGGAG CGCCTCCATA TAACCCAGCT 
CAGAATGCAG GCTCCGCCCC CCTGGTGTAC TCGCCACAGA CGCAGCCAAT GAACGTACAG 
CCACAGACTC GACCGTTTGT GACGGGACCT CGGCCAACAC ACCATCAGTT CATACACAGG 
AGTCAAATGC AGCCAGCGCG GCCTACCCTT CCGACTAACA ACCCCTCCAT TCGGCCGGGC 
TCACAGACCC CCACGGCGAC AGTTTATCCA CCAAACCAAC CCATCATGAT GACCATGACG 
CCCATGCCAT TTGCTACCCA GACACACCAA TATTACATTC CCCAGTATCG TCATAGTGCA 
CCTTACGTGG GACCCCCGCA GCAGTATGCC GTCCAGCCTC CAGGGTCTGG AACTTTCTAC 
CCTGGACCCA GCCCTGCGGA ATATCCCACC CCTTATGCTG CTGGTCCGCC ATATTACACC 
GGGCAGACGG TGTACCCACC CTCACCACCC ATCATAGTGC CTGCACCAAT GCCACCTCCA 
CCAACCAAGC GAGAAAAGAA ACCAATCCGG ATCCGAGACC CCAACCAGGG TGGAAAGGAC 
ATCACAGAGG AAATCATGTT TGGGAGCCGC AATCCTACAC CTCCTGCCGG ACACCCAGCC 
TCCACCTTAA CCCCACCTGC AGGACGTCCC TCCTCGACTC CGACCCCACC TTCAGGACGC 
CTGTCCTCCA CACCCACCCC TCCACAGCGG CCCAGCAACT GTCAAACCCC AGAACAAACG 
GCATATGTCA ACCAAAATCA GAGACTCTCA GAGAGCCCTG CGCCAATGGA TGGCAAGCCC 
AGCTTAGCTA TAGACGACAG GCCAAAAATG GAGTCTGGTC CCATTAAGTC TATATCTCCT 
GGACCAAGAC CTTCAGAATC CTGTTTGGAG AAAAGAGAAA TCTCAAGTCT TCCATTACTA 
GTCTCTTCTT CTCCAGAGGT GGATGTTTCT TCCCATCCTA CAAGTGGATG CATCAAACCC 
ACTGCCGCAG GAGAACCAGA ATTTATCTCT CCTTCAGCCA CTAAGGCTCA GACGTATCAG 
GTCATCTCCG GTGAGGAATC TGTTCCTGAA GCTTCTCCCA GATTGTCTGC TTCATTGTCC 
CTCAGAGTGG TAAATGGTGT CAATGAGCCT CAAACTCCCT CAAGCTATGA AGAGCCTGAG 
GTCCAGGAAG CCCTAAAGAT GTCGTCATCC TGCGAGATTC AGGGTACATC ATTCATGGAG 
GAGTCTGGTC AGGAAGTTCC AGTGGCCTTG GAGGAGCTCC AGGCAGAGCA CCTCCCTTCT 
CTAGCTGCAC ATGTGCCCTT GATCCCAGGC GTACAGGCCT CTTCAATCAC CTCCAGCACC 
ACTTCTGTTC TTGCGCCACC ACCTGGGTTG GCACCATTAG TGCAGTCGGT TGTCCTTGAG 
GTGTCAGAAC AGAACAAGAC CTCAGACGAT GTCCAGCTGA AAGCTCAAGA TGCTTCAGAA 
TCACAAACTC AAAGCAACTC TAGAAAGTTT ACACCAGCCC AAGCTGTCCC TGTTGTACCA 
AAGCCTTGGA AGAAACCAAA CGACGAAGAT CTTATGATGG AAACGCTGAA GAAAGACAAT 
GAGGAGGAGA CCTCAAAGGA CTGCTCTGCT GGAGATGAGA TGGTCTTGCA GTCAAGCTTA 
GGGACCTACA GCAGCCCCTT TACAGTGCCT GAGCACCAGG AGAAGCACTC TGAAGAAAAC 
GGTGAGACCG AGACAGAGCC TTTCAGGAAC GGGGTTGAGA CGGAGAGCAC GGACAGTGGT 
GTGACGCTCG GGGACAGTAG GGGCTCCATG TGTTCCCCAA CTCCACTCAT TCAGGAAGGT 
CTTGTGCAAT CTAATGGAAA GAGACAGTAT GACCGGGACT TCCTGTTGGG CTTTCAGTTT 
ATGCCGGCCT GTGTGCAGAA GCCCGAGGGA CTGCCTCCAA TCAGTGATGT GGTGCTAGAC 
AAGATGAACC AAAACAAGCT GCCTCTGAGG TCTGTGGACC CCAGGTTGAT TTCCAGAGGG 
ACGCCTCAGG ATTTCACTCC TGCTTTTGCT GATTTCAGCA GGCAGATGGC TGGAGGACGC 
GGAGCCCCGC TTTTAAATGT GCGACGACTC CCACCACGCA AGATCATCGT AAACCTGTCT 
GTCAGTGATG ATGTTCAGCT CAAGAAGGCA GAAAATGCTT GGAAACCTGG GATGAAGAGA 
GAGGGTGTCG CAGAGGACCC AGAAACCATC AGAACCCAGG AGTTGTTCCG GAAAGTTCGT 
AGCATTTTAA ACAAGCTCAC GCCGCAGATG TTTCATCAGC TGATGAAGCA AGTGACGGAT 
CTTACTATCA ACACAGAGGA GAGGCTTAAA GGAGTCATCG ATCTTGTGTT TGAGAAGGCC 
ATCGATGAGC CCAGCTTCTC TGTGGCCTAC GCCAACATGT GTCGCTGCCT GGCCACGCTC 
AAAGTGCCCA CAGCAGACAA ACCTAATACA ACGGTGAACT TCCGGAAGCT CCTGTTGAAT 
CGCTGTCAAA AGGAATTCGA GAGGGACAAA GTAGATGACG TTGTCCTGGA GAGAAAACAA 
AAGGAGATTG ACTCTGCCAC ATCACCCACA GAAAAGGAAA GACTGCAGGA GGAGCTGGAG 
GAGGCGAAGG ACAAGGCCAG ACGTCGCTCC ACTGGAAATA TCAAATTCAT CGGTGAGCTG 
TTCAAGCTCA AGATGTTGAC AGAGCCCATC ATGCATGACT GCGTGGTGAA ACTGCTCAAA 
AACCACGATG ATGAGAGCTT AGAGTGCCTC TGCAGACTCC TCACCACCAT CGGCAAAGAC 
CTGGACTTCG AGAAGGCCAA GCCCCGAATG GACCAGTACT TCAACCAGAT GGAGAAAATT 
GTCAAAGAGC GCAAGACCTC GTCTCGGATA CGGTTCATGC TGCAGGATGT GATAGATCTG 
AGACTGCATA ACTGGGTTTC TCGGAGAGCT GACCAAGGCC CTAAAACCAT CGAGCAGATC 
CATAAAGAGG CCAAGCTGGA GGAGCAGGAG GAGCAGAGGA AAGTCCACCA GCAGCTTTTG 
TCTAAAGACA TGAAGAGGAG GCCAGATCAG AGAGAACAGC GTTCAATGAG AGAGGAGACT 
TGGAACACTG TGCCCACTAC CAAAAACAGC AGGACTATCG ACCCTACAAA GATTCCAAAG 
ATCTCCAAGC CGCAAATCGA TGAAAAGATT CAGCTGGGTC CGCGAGCTCA GATCAACTGG 
GTGAAAGGCA GCAGCGGCGG AGCAAAAGCC AGCGACTCTG AGTTGACCCG CTCTGCCGGA 
AACACTCTGA ACCGCTATTC AGCACTGCAG TCATCCACGT CTCAATCCAG TTCAACTCCG 
GCCCAAACCT CTGAATCTGA GTCCAGACGC TCGCTGGGAA GTCGAGGCAG TATGGGTCGC 
GAGCGCAGTG AAAAGCCTGT GAGTTTGGGG CCGTCACGAC CGGGTCCTTT TGGCAGAGGT 
AGCAGCGCTA AGGAGCTAAC AGATAACATG GTCCCAGAGG AAACACGGAG AGAAGGACAA 
GACTCACACC ACACATCTCG CAAAGCCAGC ATCTCAGAGG AGAAATGTGA GATGGAGAAC 
AGCAGATCCG CAGAGTCAGT GAAATCAGAG ACGCTGCTGG TCAGCGGTGT TTCAGAGAAG 
CCTGTCCTGT CTGAAGAGGA GATCGAGCGG AAGTCCAAAG CTATCATTGA TGAATTTCTG 
CACATTAATG ACTACAAGGA GGCAGTGCAG TGTGTGCTGG AGATAGAGCA GCCCTCTATG 
CTGTGTGTGT TTGTGCGCAT GGGTCTGGAG AGCACACTGG AGCGCAGTCA GAAAGCCAGA 
GAGCACATGG GACTGCTCTA CTATCAGCTT ATACAAAAGG GCATCTTACC CCATTCTCAA 
CTCTACAAAG GGTTTTCAGA GATGTTGGAG CAGGCTGATG ACATGGCCAT AGACATTCCC 
TTCATTTGGC TTTATCTGGC TGAGCTGCTC AGTCCTCTGT TGAAAGAGGG AGGAATCAAC 
ATGAGAGAGC TGTTCAGTGA GTTAAGTAAA CCGCTACTCC CTGTGGGAAG AGCTGCCATC 
TTATTGTCTG AAATACTGCA CCTACTATGC AAACAAATGA GCCATAGGAA AGTTGGAGAC 
TTGTGGAGAG AGTCTGGGCT GAACTGGGCC GACTTCCTGC CCGAGGGAGA ACATCTTCCT 
GCCTTCATCT CACAACATAA GTTAGAATTC ACAGTGTCGG ACATCTCTCC TGTCTCTCCG 
GCCTCAGCCA AACCCAGCCT CAGTCCTGAG GAGCTCTTTA AACAACTGGA GCAGCTCCTG 
CTAGAGGACA TGTCCAGCGA TGAACAGATT TTTGACTGGA TCGAGGCTAA TTTGGACGAA 
TCTCAGATGA GTTCTTCTCC TTTTTTGAGA GCTTTAATGA CTGCTATATG TAAAGCAGCA 
GTTAAAGATG AAAGCACGTC CTGTCGAGTG GACACTGCTA TCATCCAGAA ACGCCTGCCT 
ATATTACATA AGTACTTCGA CTCGGACACC GAGCGGCAGC TGCAAGCACT TTATGCTCTT 
CAGTCTCTGA TTGTCGCCCT GGATCAACCA CCCAACCTAC TCCGGATGTT TTTCGATTGT 
CTCTACGATG AGGACGTCAT TTCAGAGGAC GCTTTCTATC AATGGGAGAC GAGTAAAGAC 
CCGACAGAGC AGCTGGGAAA AGGCGTAGCG CTGAAGTCTG TGAACGCTTT CTTCACCTGG 
TTACGTGAAG CCGAGGAAGA ATCTGAGGAT AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_694490.5
CDS	460..5595
Translation	MSLPPKVVPKPAAVAVSGHVTGPAPPTQLRAALTSVSLPPGAQNAPPSAVPPTQIPRAALSLDERMFPAHSGVTAVYSVSRHPGPPFPGHDLSKTHPNLAGTPPGHATSPALSQVSVPAGPSYRILKPWETGGAPPYNPAQNAGSAPLVYSPQTQPMNVQPQTRPFVTGPRPTHHQFIHRSQMQPARPTLPTNNPSIRPGSQTPTATVYPPNQPIMMTMTPMPFATQTHQYYIPQYRHSAPYVGPPQQYAVQPPGSGTFYPGPSPAEYPTPYAAGPPYYTGQTVYPPSPPIIVPAPMPPPPTKREKKPIRIRDPNQGGKDITEEIMFGSRNPTPPAGHPASTLTPPAGRPSSTPTPPSGRLSSTPTPPQRPSNCQTPEQTAYVNQNQRLSESPAPMDGKPSLAIDDRPKMESGPIKSISPGPRPSESCLEKREISSLPLLVSSSPEVDVSSHPTSGCIKPTAAGEPEFISPSATKAQTYQVISGEESVPEASPRLSASLSLRVVNGVNEPQTPSSYEEPEVQEALKMSSSCEIQGTSFMEESGQEVPVALEELQAEHLPSLAAHVPLIPGVQASSITSSTTSVLAPPPGLAPLVQSVVLEVSEQNKTSDDVQLKAQDASESQTQSNSRKFTPAQAVPVVPKPWKKPNDEDLMMETLKKDNEEETSKDCSAGDEMVLQSSLGTYSSPFTVPEHQEKHSEENGETETEPFRNGVETESTDSGVTLGDSRGSMCSPTPLIQEGLVQSNGKRQYDRDFLLGFQFMPACVQKPEGLPPISDVVLDKMNQNKLPLRSVDPRLISRGTPQDFTPAFADFSRQMAGGRGAPLLNVRRLPPRKIIVNLSVSDDVQLKKAENAWKPGMKREGVAEDPETIRTQELFRKVRSILNKLTPQMFHQLMKQVTDLTINTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLATLKVPTADKPNTTVNFRKLLLNRCQKEFERDKVDDVVLERKQKEIDSATSPTEKERLQEELEEAKDKARRRSTGNIKFIGELFKLKMLTEPIMHDCVVKLLKNHDDESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLHNWVSRRADQGPKTIEQIHKEAKLEEQEEQRKVHQQLLSKDMKRRPDQREQRSMREETWNTVPTTKNSRTIDPTKIPKISKPQIDEKIQLGPRAQINWVKGSSGGAKASDSELTRSAGNTLNRYSALQSSTSQSSSTPAQTSESESRRSLGSRGSMGRERSEKPVSLGPSRPGPFGRGSSAKELTDNMVPEETRREGQDSHHTSRKASISEEKCEMENSRSAESVKSETLLVSGVSEKPVLSEEEIERKSKAIIDEFLHINDYKEAVQCVLEIEQPSMLCVFVRMGLESTLERSQKAREHMGLLYYQLIQKGILPHSQLYKGFSEMLEQADDMAIDIPFIWLYLAELLSPLLKEGGINMRELFSELSKPLLPVGRAAILLSEILHLLCKQMSHRKVGDLWRESGLNWADFLPEGEHLPAFISQHKLEFTVSDISPVSPASAKPSLSPEELFKQLEQLLLEDMSSDEQIFDWIEANLDESQMSSSPFLRALMTAICKAAVKDESTSCRVDTAIIQKRLPILHKYFDSDTERQLQALYALQSLIVALDQPPNLLRMFFDCLYDEDVISEDAFYQWETSKDPTEQLGKGVALKSVNAFFTWLREAEEESEDN

Target ORF information:

RefSeq Version	XM_689398.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio eukaryotic translation initiation factor 4 gamma, 3a (eif4g3a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_689398.8

ORF Insert Sequence:

ATGAGTTTGC CACCGAAAGT GGTTCCGAAA CCGGCCGCTG TCGCTGTTAG TGGACACGTA 
ACCGGACCGG CACCGCCAAC GCAGCTCCGC GCAGCGCTGA CCTCCGTGTC GTTGCCACCG 
GGAGCCCAAA ACGCTCCGCC GTCAGCCGTG CCGCCTACCC AGATTCCTAG AGCAGCGCTA 
TCACTGGATG AACGCATGTT TCCCGCTCAC TCTGGGGTGA CTGCGGTCTA CAGTGTATCC 
AGACACCCTG GTCCTCCATT TCCAGGTCAT GACCTCAGCA AAACCCACCC AAACCTGGCA 
GGGACTCCTC CTGGCCATGC CACGTCCCCG GCCCTCTCTC AGGTATCAGT TCCTGCTGGC 
CCTTCGTACC GCATCCTCAA GCCCTGGGAG ACAGGCGGAG CGCCTCCATA TAACCCAGCT 
CAGAATGCAG GCTCCGCCCC CCTGGTGTAC TCGCCACAGA CGCAGCCAAT GAACGTACAG 
CCACAGACTC GACCGTTTGT GACGGGACCT CGGCCAACAC ACCATCAGTT CATACACAGG 
AGTCAAATGC AGCCAGCGCG GCCTACCCTT CCGACTAACA ACCCCTCCAT TCGGCCGGGC 
TCACAGACCC CCACGGCGAC AGTTTATCCA CCAAACCAAC CCATCATGAT GACCATGACG 
CCCATGCCAT TTGCTACCCA GACACACCAA TATTACATTC CCCAGTATCG TCATAGTGCA 
CCTTACGTGG GACCCCCGCA GCAGTATGCC GTCCAGCCTC CAGGGTCTGG AACTTTCTAC 
CCTGGACCCA GCCCTGCGGA ATATCCCACC CCTTATGCTG CTGGTCCGCC ATATTACACC 
GGGCAGACGG TGTACCCACC CTCACCACCC ATCATAGTGC CTGCACCAAT GCCACCTCCA 
CCAACCAAGC GAGAAAAGAA ACCAATCCGG ATCCGAGACC CCAACCAGGG TGGAAAGGAC 
ATCACAGAGG AAATCATGTT TGGGAGCCGC AATCCTACAC CTCCTGCCGG ACACCCAGCC 
TCCACCTTAA CCCCACCTGC AGGACGTCCC TCCTCGACTC CGACCCCACC TTCAGGACGC 
CTGTCCTCCA CACCCACCCC TCCACAGCGG CCCAGCAACT GTCAAACCCC AGAACAAACG 
GCATATGTCA ACCAAAATCA GAGACTCTCA GAGAGCCCTG CGCCAATGGA TGGCAAGCCC 
AGCTTAGCTA TAGACGACAG GCCAAAAATG GAGTCTGGTC CCATTAAGTC TATATCTCCT 
GGACCAAGAC CTTCAGAATC CTGTTTGGAG AAAAGAGAAA TCTCAAGTCT TCCATTACTA 
GTCTCTTCTT CTCCAGAGGT GGATGTTTCT TCCCATCCTA CAAGTGGATG CATCAAACCC 
ACTGCCGCAG GAGAACCAGA ATTTATCTCT CCTTCAGCCA CTAAGGCTCA GACGTATCAG 
GTCATCTCCG GTGAGGAATC TGTTCCTGAA GCTTCTCCCA GATTGTCTGC TTCATTGTCC 
CTCAGAGTGG TAAATGGTGT CAATGAGCCT CAAACTCCCT CAAGCTATGA AGAGCCTGAG 
GTCCAGGAAG CCCTAAAGAT GTCGTCATCC TGCGAGATTC AGGGTACATC ATTCATGGAG 
GAGTCTGGTC AGGAAGTTCC AGTGGCCTTG GAGGAGCTCC AGGCAGAGCA CCTCCCTTCT 
CTAGCTGCAC ATGTGCCCTT GATCCCAGGC GTACAGGCCT CTTCAATCAC CTCCAGCACC 
ACTTCTGTTC TTGCGCCACC ACCTGGGTTG GCACCATTAG TGCAGTCGGT TGTCCTTGAG 
GTGTCAGAAC AGAACAAGAC CTCAGACGAT GTCCAGCTGA AAGCTCAAGA TGCTTCAGAA 
TCACAAACTC AAAGCAACTC TAGAAAGTTT ACACCAGCCC AAGCTGTCCC TGTTGTACCA 
AAGCCTTGGA AGAAACCAAA CGACGAAGAT CTTATGATGG AAACGCTGAA GAAAGACAAT 
GAGGAGGAGA CCTCAAAGGA CTGCTCTGCT GGAGATGAGA TGGTCTTGCA GTCAAGCTTA 
GGGACCTACA GCAGCCCCTT TACAGTGCCT GAGCACCAGG AGAAGCACTC TGAAGAAAAC 
GGTGAGACCG AGACAGAGCC TTTCAGGAAC GGGGTTGAGA CGGAGAGCAC GGACAGTGGT 
GTGACGCTCG GGGACAGTAG GGGCTCCATG TGTTCCCCAA CTCCACTCAT TCAGGAAGGT 
CTTGTGCAAT CTAATGGAAA GAGACAGTAT GACCGGGACT TCCTGTTGGG CTTTCAGTTT 
ATGCCGGCCT GTGTGCAGAA GCCCGAGGGA CTGCCTCCAA TCAGTGATGT GGTGCTAGAC 
AAGATGAACC AAAACAAGCT GCCTCTGAGG TCTGTGGACC CCAGGTTGAT TTCCAGAGGG 
ACGCCTCAGG ATTTCACTCC TGCTTTTGCT GATTTCAGCA GGCAGATGGC TGGAGGACGC 
GGAGCCCCGC TTTTAAATGT GCGACGACTC CCACCACGCA AGATCATCGT AAACCTGTCT 
GTCAGTGATG ATGTTCAGCT CAAGAAGGCA GAAAATGCTT GGAAACCTGG GATGAAGAGA 
GAGGGTGTCG CAGAGGACCC AGAAACCATC AGAACCCAGG AGTTGTTCCG GAAAGTTCGT 
AGCATTTTAA ACAAGCTCAC GCCGCAGATG TTTCATCAGC TGATGAAGCA AGTGACGGAT 
CTTACTATCA ACACAGAGGA GAGGCTTAAA GGAGTCATCG ATCTTGTGTT TGAGAAGGCC 
ATCGATGAGC CCAGCTTCTC TGTGGCCTAC GCCAACATGT GTCGCTGCCT GGCCACGCTC 
AAAGTGCCCA CAGCAGACAA ACCTAATACA ACGGTGAACT TCCGGAAGCT CCTGTTGAAT 
CGCTGTCAAA AGGAATTCGA GAGGGACAAA GTAGATGACG TTGTCCTGGA GAGAAAACAA 
AAGGAGATTG ACTCTGCCAC ATCACCCACA GAAAAGGAAA GACTGCAGGA GGAGCTGGAG 
GAGGCGAAGG ACAAGGCCAG ACGTCGCTCC ACTGGAAATA TCAAATTCAT CGGTGAGCTG 
TTCAAGCTCA AGATGTTGAC AGAGCCCATC ATGCATGACT GCGTGGTGAA ACTGCTCAAA 
AACCACGATG ATGAGAGCTT AGAGTGCCTC TGCAGACTCC TCACCACCAT CGGCAAAGAC 
CTGGACTTCG AGAAGGCCAA GCCCCGAATG GACCAGTACT TCAACCAGAT GGAGAAAATT 
GTCAAAGAGC GCAAGACCTC GTCTCGGATA CGGTTCATGC TGCAGGATGT GATAGATCTG 
AGACTGCATA ACTGGGTTTC TCGGAGAGCT GACCAAGGCC CTAAAACCAT CGAGCAGATC 
CATAAAGAGG CCAAGCTGGA GGAGCAGGAG GAGCAGAGGA AAGTCCACCA GCAGCTTTTG 
TCTAAAGACA TGAAGAGGAG GCCAGATCAG AGAGAACAGC GTTCAATGAG AGAGGAGACT 
TGGAACACTG TGCCCACTAC CAAAAACAGC AGGACTATCG ACCCTACAAA GATTCCAAAG 
ATCTCCAAGC CGCAAATCGA TGAAAAGATT CAGCTGGGTC CGCGAGCTCA GATCAACTGG 
GTGAAAGGCA GCAGCGGCGG AGCAAAAGCC AGCGACTCTG AGTTGACCCG CTCTGCCGGA 
AACACTCTGA ACCGCTATTC AGCACTGCAG TCATCCACGT CTCAATCCAG TTCAACTCCG 
GCCCAAACCT CTGAATCTGA GTCCAGACGC TCGCTGGGAA GTCGAGGCAG TATGGGTCGC 
GAGCGCAGTG AAAAGCCTGT GAGTTTGGGG CCGTCACGAC CGGGTCCTTT TGGCAGAGGT 
AGCAGCGCTA AGGAGCTAAC AGATAACATG GTCCCAGAGG AAACACGGAG AGAAGGACAA 
GACTCACACC ACACATCTCG CAAAGCCAGC ATCTCAGAGG AGAAATGTGA GATGGAGAAC 
AGCAGATCCG CAGAGTCAGT GAAATCAGAG ACGCTGCTGG TCAGCGGTGT TTCAGAGAAG 
CCTGTCCTGT CTGAAGAGGA GATCGAGCGG AAGTCCAAAG CTATCATTGA TGAATTTCTG 
CACATTAATG ACTACAAGGA GGCAGTGCAG TGTGTGCTGG AGATAGAGCA GCCCTCTATG 
CTGTGTGTGT TTGTGCGCAT GGGTCTGGAG AGCACACTGG AGCGCAGTCA GAAAGCCAGA 
GAGCACATGG GACTGCTCTA CTATCAGCTT ATACAAAAGG GCATCTTACC CCATTCTCAA 
CTCTACAAAG GGTTTTCAGA GATGTTGGAG CAGGCTGATG ACATGGCCAT AGACATTCCC 
TTCATTTGGC TTTATCTGGC TGAGCTGCTC AGTCCTCTGT TGAAAGAGGG AGGAATCAAC 
ATGAGAGAGC TGTTCAGTGA GTTAAGTAAA CCGCTACTCC CTGTGGGAAG AGCTGCCATC 
TTATTGTCTG AAATACTGCA CCTACTATGC AAACAAATGA GCCATAGGAA AGTTGGAGAC 
TTGTGGAGAG AGTCTGGGCT GAACTGGGCC GACTTCCTGC CCGAGGGAGA ACATCTTCCT 
GCCTTCATCT CACAACATAA GTTAGAATTC ACAGTGTCGG ACATCTCTCC TGTCTCTCCG 
GCCTCAGCCA AACCCAGCCT CAGTCCTGAG GAGCTCTTTA AACAACTGGA GCAGCTCCTG 
CTAGAGGACA TGTCCAGCGA TGAACAGATT TTTGACTGGA TCGAGGCTAA TTTGGACGAA 
TCTCAGATGA GTTCTTCTCC TTTTTTGAGA GCTTTAATGA CTGCTATATG TAAAGCAGCA 
GTTAAAGATG AAAGCACGTC CTGTCGAGTG GACACTGCTA TCATCCAGAA ACGCCTGCCT 
ATATTACATA AGTACTTCGA CTCGGACACC GAGCGGCAGC TGCAAGCACT TTATGCTCTT 
CAGTCTCTGA TTGTCGCCCT GGATCAACCA CCCAACCTAC TCCGGATGTT TTTCGATTGT 
CTCTACGATG AGGACGTCAT TTCAGAGGAC GCTTTCTATC AATGGGAGAC GAGTAAAGAC 
CCGACAGAGC AGCTGGGAAA AGGCGTAGCG CTGAAGTCTG TGAACGCTTT CTTCACCTGG 
TTACGTGAAG CCGAGGAAGA ATCTGAGGAT AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G3A ( XM_689398.7 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_694490.5 Danio rerio eukaryotic translation initiation factor 4 gamma, 3a (eif4g3a), transcript variant X3, mRNA.

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CloneID	ODa17153
Clone ID Related Accession (Same CDS sequence)	XM_689398.8 , XM_689398.7
Accession Version	XM_689398.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	eukaryotic translation initiation factor 4 gamma 3 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_689398.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTTGC CACCGAAAGT GGTTCCGAAA CCGGCCGCTG TCGCTGTTAG TGGACACGTA 
ACCGGACCGG CACCGCCAAC GCAGCTCCGC GCAGCGCTGA CCTCCGTGTC GTTGCCACCG 
GGAGCCCAAA ACGCTCCGCC GTCAGCCGTG CCGCCTACCC AGATTCCTAG AGCAGCGCTA 
TCACTGGATG AACGCATGTT TCCCGCTCAC TCTGGGGTGA CTGCGGTCTA CAGTGTATCC 
AGACACCCTG GTCCTCCATT TCCAGGTCAT GACCTCAGCA AAACCCACCC AAACCTGGCA 
GGGACTCCTC CTGGCCATGC CACGTCCCCG GCCCTCTCTC AGGTATCAGT TCCTGCTGGC 
CCTTCGTACC GCATCCTCAA GCCCTGGGAG ACAGGCGGAG CGCCTCCATA TAACCCAGCT 
CAGAATGCAG GCTCCGCCCC CCTGGTGTAC TCGCCACAGA CGCAGCCAAT GAACGTACAG 
CCACAGACTC GACCGTTTGT GACGGGACCT CGGCCAACAC ACCATCAGTT CATACACAGG 
AGTCAAATGC AGCCAGCGCG GCCTACCCTT CCGACTAACA ACCCCTCCAT TCGGCCGGGC 
TCACAGACCC CCACGGCGAC AGTTTATCCA CCAAACCAAC CCATCATGAT GACCATGACG 
CCCATGCCAT TTGCTACCCA GACACACCAA TATTACATTC CCCAGTATCG TCATAGTGCA 
CCTTACGTGG GACCCCCGCA GCAGTATGCC GTCCAGCCTC CAGGGTCTGG AACTTTCTAC 
CCTGGACCCA GCCCTGCGGA ATATCCCACC CCTTATGCTG CTGGTCCGCC ATATTACACC 
GGGCAGACGG TGTACCCACC CTCACCACCC ATCATAGTGC CTGCACCAAT GCCACCTCCA 
CCAACCAAGC GAGAAAAGAA ACCAATCCGG ATCCGAGACC CCAACCAGGG TGGAAAGGAC 
ATCACAGAGG AAATCATGTT TGGGAGCCGC AATCCTACAC CTCCTGCCGG ACACCCAGCC 
TCCACCTTAA CCCCACCTGC AGGACGTCCC TCCTCGACTC CGACCCCACC TTCAGGACGC 
CTGTCCTCCA CACCCACCCC TCCACAGCGG CCCAGCAACT GTCAAACCCC AGAACAAACG 
GCATATGTCA ACCAAAATCA GAGACTCTCA GAGAGCCCTG CGCCAATGGA TGGCAAGCCC 
AGCTTAGCTA TAGACGACAG GCCAAAAATG GAGTCTGGTC CCATTAAGTC TATATCTCCT 
GGACCAAGAC CTTCAGAATC CTGTTTGGAG AAAAGAGAAA TCTCAAGTCT TCCATTACTA 
GTCTCTTCTT CTCCAGAGGT GGATGTTTCT TCCCATCCTA CAAGTGGATG CATCAAACCC 
ACTGCCGCAG GAGAACCAGA ATTTATCTCT CCTTCAGCCA CTAAGGCTCA GACGTATCAG 
GTCATCTCCG GTGAGGAATC TGTTCCTGAA GCTTCTCCCA GATTGTCTGC TTCATTGTCC 
CTCAGAGTGG TAAATGGTGT CAATGAGCCT CAAACTCCCT CAAGCTATGA AGAGCCTGAG 
GTCCAGGAAG CCCTAAAGAT GTCGTCATCC TGCGAGATTC AGGGTACATC ATTCATGGAG 
GAGTCTGGTC AGGAAGTTCC AGTGGCCTTG GAGGAGCTCC AGGCAGAGCA CCTCCCTTCT 
CTAGCTGCAC ATGTGCCCTT GATCCCAGGC GTACAGGCCT CTTCAATCAC CTCCAGCACC 
ACTTCTGTTC TTGCGCCACC ACCTGGGTTG GCACCATTAG TGCAGTCGGT TGTCCTTGAG 
GTGTCAGAAC AGAACAAGAC CTCAGACGAT GTCCAGCTGA AAGCTCAAGA TGCTTCAGAA 
TCACAAACTC AAAGCAACTC TAGAAAGTTT ACACCAGCCC AAGCTGTCCC TGTTGTACCA 
AAGCCTTGGA AGAAACCAAA CGACGAAGAT CTTATGATGG AAACGCTGAA GAAAGACAAT 
GAGGAGGAGA CCTCAAAGGA CTGCTCTGCT GGAGATGAGA TGGTCTTGCA GTCAAGCTTA 
GGGACCTACA GCAGCCCCTT TACAGTGCCT GAGCACCAGG AGAAGCACTC TGAAGAAAAC 
GGTGAGACCG AGACAGAGCC TTTCAGGAAC GGGGTTGAGA CGGAGAGCAC GGACAGTGGT 
GTGACGCTCG GGGACAGTAG GGGCTCCATG TGTTCCCCAA CTCCACTCAT TCAGGAAGGT 
CTTGTGCAAT CTAATGGAAA GAGACAGTAT GACCGGGACT TCCTGTTGGG CTTTCAGTTT 
ATGCCGGCCT GTGTGCAGAA GCCCGAGGGA CTGCCTCCAA TCAGTGATGT GGTGCTAGAC 
AAGATGAACC AAAACAAGCT GCCTCTGAGG TCTGTGGACC CCAGGTTGAT TTCCAGAGGG 
ACGCCTCAGG ATTTCACTCC TGCTTTTGCT GATTTCAGCA GGCAGATGGC TGGAGGACGC 
GGAGCCCCGC TTTTAAATGT GCGACGACTC CCACCACGCA AGATCATCGT AAACCTGTCT 
GTCAGTGATG ATGTTCAGCT CAAGAAGGCA GAAAATGCTT GGAAACCTGG GATGAAGAGA 
GAGGGTGTCG CAGAGGACCC AGAAACCATC AGAACCCAGG AGTTGTTCCG GAAAGTTCGT 
AGCATTTTAA ACAAGCTCAC GCCGCAGATG TTTCATCAGC TGATGAAGCA AGTGACGGAT 
CTTACTATCA ACACAGAGGA GAGGCTTAAA GGAGTCATCG ATCTTGTGTT TGAGAAGGCC 
ATCGATGAGC CCAGCTTCTC TGTGGCCTAC GCCAACATGT GTCGCTGCCT GGCCACGCTC 
AAAGTGCCCA CAGCAGACAA ACCTAATACA ACGGTGAACT TCCGGAAGCT CCTGTTGAAT 
CGCTGTCAAA AGGAATTCGA GAGGGACAAA GTAGATGACG TTGTCCTGGA GAGAAAACAA 
AAGGAGATTG ACTCTGCCAC ATCACCCACA GAAAAGGAAA GACTGCAGGA GGAGCTGGAG 
GAGGCGAAGG ACAAGGCCAG ACGTCGCTCC ACTGGAAATA TCAAATTCAT CGGTGAGCTG 
TTCAAGCTCA AGATGTTGAC AGAGCCCATC ATGCATGACT GCGTGGTGAA ACTGCTCAAA 
AACCACGATG ATGAGAGCTT AGAGTGCCTC TGCAGACTCC TCACCACCAT CGGCAAAGAC 
CTGGACTTCG AGAAGGCCAA GCCCCGAATG GACCAGTACT TCAACCAGAT GGAGAAAATT 
GTCAAAGAGC GCAAGACCTC GTCTCGGATA CGGTTCATGC TGCAGGATGT GATAGATCTG 
AGACTGCATA ACTGGGTTTC TCGGAGAGCT GACCAAGGCC CTAAAACCAT CGAGCAGATC 
CATAAAGAGG CCAAGCTGGA GGAGCAGGAG GAGCAGAGGA AAGTCCACCA GCAGCTTTTG 
TCTAAAGACA TGAAGAGGAG GCCAGATCAG AGAGAACAGC GTTCAATGAG AGAGGAGACT 
TGGAACACTG TGCCCACTAC CAAAAACAGC AGGACTATCG ACCCTACAAA GATTCCAAAG 
ATCTCCAAGC CGCAAATCGA TGAAAAGATT CAGCTGGGTC CGCGAGCTCA GATCAACTGG 
GTGAAAGGCA GCAGCGGCGG AGCAAAAGCC AGCGACTCTG AGTTGACCCG CTCTGCCGGA 
AACACTCTGA ACCGCTATTC AGCACTGCAG TCATCCACGT CTCAATCCAG TTCAACTCCG 
GCCCAAACCT CTGAATCTGA GTCCAGACGC TCGCTGGGAA GTCGAGGCAG TATGGGTCGC 
GAGCGCAGTG AAAAGCCTGT GAGTTTGGGG CCGTCACGAC CGGGTCCTTT TGGCAGAGGT 
AGCAGCGCTA AGGAGCTAAC AGATAACATG GTCCCAGAGG AAACACGGAG AGAAGGACAA 
GACTCACACC ACACATCTCG CAAAGCCAGC ATCTCAGAGG AGAAATGTGA GATGGAGAAC 
AGCAGATCCG CAGAGTCAGT GAAATCAGAG ACGCTGCTGG TCAGCGGTGT TTCAGAGAAG 
CCTGTCCTGT CTGAAGAGGA GATCGAGCGG AAGTCCAAAG CTATCATTGA TGAATTTCTG 
CACATTAATG ACTACAAGGA GGCAGTGCAG TGTGTGCTGG AGATAGAGCA GCCCTCTATG 
CTGTGTGTGT TTGTGCGCAT GGGTCTGGAG AGCACACTGG AGCGCAGTCA GAAAGCCAGA 
GAGCACATGG GACTGCTCTA CTATCAGCTT ATACAAAAGG GCATCTTACC CCATTCTCAA 
CTCTACAAAG GGTTTTCAGA GATGTTGGAG CAGGCTGATG ACATGGCCAT AGACATTCCC 
TTCATTTGGC TTTATCTGGC TGAGCTGCTC AGTCCTCTGT TGAAAGAGGG AGGAATCAAC 
ATGAGAGAGC TGTTCAGTGA GTTAAGTAAA CCGCTACTCC CTGTGGGAAG AGCTGCCATC 
TTATTGTCTG AAATACTGCA CCTACTATGC AAACAAATGA GCCATAGGAA AGTTGGAGAC 
TTGTGGAGAG AGTCTGGGCT GAACTGGGCC GACTTCCTGC CCGAGGGAGA ACATCTTCCT 
GCCTTCATCT CACAACATAA GTTAGAATTC ACAGTGTCGG ACATCTCTCC TGTCTCTCCG 
GCCTCAGCCA AACCCAGCCT CAGTCCTGAG GAGCTCTTTA AACAACTGGA GCAGCTCCTG 
CTAGAGGACA TGTCCAGCGA TGAACAGATT TTTGACTGGA TCGAGGCTAA TTTGGACGAA 
TCTCAGATGA GTTCTTCTCC TTTTTTGAGA GCTTTAATGA CTGCTATATG TAAAGCAGCA 
GTTAAAGATG AAAGCACGTC CTGTCGAGTG GACACTGCTA TCATCCAGAA ACGCCTGCCT 
ATATTACATA AGTACTTCGA CTCGGACACC GAGCGGCAGC TGCAAGCACT TTATGCTCTT 
CAGTCTCTGA TTGTCGCCCT GGATCAACCA CCCAACCTAC TCCGGATGTT TTTCGATTGT 
CTCTACGATG AGGACGTCAT TTCAGAGGAC GCTTTCTATC AATGGGAGAC GAGTAAAGAC 
CCGACAGAGC AGCTGGGAAA AGGCGTAGCG CTGAAGTCTG TGAACGCTTT CTTCACCTGG 
TTACGTGAAG CCGAGGAAGA ATCTGAGGAT AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_694490.5
CDS	488..5623
Translation	MSLPPKVVPKPAAVAVSGHVTGPAPPTQLRAALTSVSLPPGAQNAPPSAVPPTQIPRAALSLDERMFPAHSGVTAVYSVSRHPGPPFPGHDLSKTHPNLAGTPPGHATSPALSQVSVPAGPSYRILKPWETGGAPPYNPAQNAGSAPLVYSPQTQPMNVQPQTRPFVTGPRPTHHQFIHRSQMQPARPTLPTNNPSIRPGSQTPTATVYPPNQPIMMTMTPMPFATQTHQYYIPQYRHSAPYVGPPQQYAVQPPGSGTFYPGPSPAEYPTPYAAGPPYYTGQTVYPPSPPIIVPAPMPPPPTKREKKPIRIRDPNQGGKDITEEIMFGSRNPTPPAGHPASTLTPPAGRPSSTPTPPSGRLSSTPTPPQRPSNCQTPEQTAYVNQNQRLSESPAPMDGKPSLAIDDRPKMESGPIKSISPGPRPSESCLEKREISSLPLLVSSSPEVDVSSHPTSGCIKPTAAGEPEFISPSATKAQTYQVISGEESVPEASPRLSASLSLRVVNGVNEPQTPSSYEEPEVQEALKMSSSCEIQGTSFMEESGQEVPVALEELQAEHLPSLAAHVPLIPGVQASSITSSTTSVLAPPPGLAPLVQSVVLEVSEQNKTSDDVQLKAQDASESQTQSNSRKFTPAQAVPVVPKPWKKPNDEDLMMETLKKDNEEETSKDCSAGDEMVLQSSLGTYSSPFTVPEHQEKHSEENGETETEPFRNGVETESTDSGVTLGDSRGSMCSPTPLIQEGLVQSNGKRQYDRDFLLGFQFMPACVQKPEGLPPISDVVLDKMNQNKLPLRSVDPRLISRGTPQDFTPAFADFSRQMAGGRGAPLLNVRRLPPRKIIVNLSVSDDVQLKKAENAWKPGMKREGVAEDPETIRTQELFRKVRSILNKLTPQMFHQLMKQVTDLTINTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLATLKVPTADKPNTTVNFRKLLLNRCQKEFERDKVDDVVLERKQKEIDSATSPTEKERLQEELEEAKDKARRRSTGNIKFIGELFKLKMLTEPIMHDCVVKLLKNHDDESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLHNWVSRRADQGPKTIEQIHKEAKLEEQEEQRKVHQQLLSKDMKRRPDQREQRSMREETWNTVPTTKNSRTIDPTKIPKISKPQIDEKIQLGPRAQINWVKGSSGGAKASDSELTRSAGNTLNRYSALQSSTSQSSSTPAQTSESESRRSLGSRGSMGRERSEKPVSLGPSRPGPFGRGSSAKELTDNMVPEETRREGQDSHHTSRKASISEEKCEMENSRSAESVKSETLLVSGVSEKPVLSEEEIERKSKAIIDEFLHINDYKEAVQCVLEIEQPSMLCVFVRMGLESTLERSQKAREHMGLLYYQLIQKGILPHSQLYKGFSEMLEQADDMAIDIPFIWLYLAELLSPLLKEGGINMRELFSELSKPLLPVGRAAILLSEILHLLCKQMSHRKVGDLWRESGLNWADFLPEGEHLPAFISQHKLEFTVSDISPVSPASAKPSLSPEELFKQLEQLLLEDMSSDEQIFDWIEANLDESQMSSSPFLRALMTAICKAAVKDESTSCRVDTAIIQKRLPILHKYFDSDTERQLQALYALQSLIVALDQPPNLLRMFFDCLYDEDVISEDAFYQWETSKDPTEQLGKGVALKSVNAFFTWLREAEEESEDN

Target ORF information:

RefSeq Version	XM_689398.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio eukaryotic translation initiation factor 4 gamma, 3a (eif4g3a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_689398.7

ORF Insert Sequence:

ATGAGTTTGC CACCGAAAGT GGTTCCGAAA CCGGCCGCTG TCGCTGTTAG TGGACACGTA 
ACCGGACCGG CACCGCCAAC GCAGCTCCGC GCAGCGCTGA CCTCCGTGTC GTTGCCACCG 
GGAGCCCAAA ACGCTCCGCC GTCAGCCGTG CCGCCTACCC AGATTCCTAG AGCAGCGCTA 
TCACTGGATG AACGCATGTT TCCCGCTCAC TCTGGGGTGA CTGCGGTCTA CAGTGTATCC 
AGACACCCTG GTCCTCCATT TCCAGGTCAT GACCTCAGCA AAACCCACCC AAACCTGGCA 
GGGACTCCTC CTGGCCATGC CACGTCCCCG GCCCTCTCTC AGGTATCAGT TCCTGCTGGC 
CCTTCGTACC GCATCCTCAA GCCCTGGGAG ACAGGCGGAG CGCCTCCATA TAACCCAGCT 
CAGAATGCAG GCTCCGCCCC CCTGGTGTAC TCGCCACAGA CGCAGCCAAT GAACGTACAG 
CCACAGACTC GACCGTTTGT GACGGGACCT CGGCCAACAC ACCATCAGTT CATACACAGG 
AGTCAAATGC AGCCAGCGCG GCCTACCCTT CCGACTAACA ACCCCTCCAT TCGGCCGGGC 
TCACAGACCC CCACGGCGAC AGTTTATCCA CCAAACCAAC CCATCATGAT GACCATGACG 
CCCATGCCAT TTGCTACCCA GACACACCAA TATTACATTC CCCAGTATCG TCATAGTGCA 
CCTTACGTGG GACCCCCGCA GCAGTATGCC GTCCAGCCTC CAGGGTCTGG AACTTTCTAC 
CCTGGACCCA GCCCTGCGGA ATATCCCACC CCTTATGCTG CTGGTCCGCC ATATTACACC 
GGGCAGACGG TGTACCCACC CTCACCACCC ATCATAGTGC CTGCACCAAT GCCACCTCCA 
CCAACCAAGC GAGAAAAGAA ACCAATCCGG ATCCGAGACC CCAACCAGGG TGGAAAGGAC 
ATCACAGAGG AAATCATGTT TGGGAGCCGC AATCCTACAC CTCCTGCCGG ACACCCAGCC 
TCCACCTTAA CCCCACCTGC AGGACGTCCC TCCTCGACTC CGACCCCACC TTCAGGACGC 
CTGTCCTCCA CACCCACCCC TCCACAGCGG CCCAGCAACT GTCAAACCCC AGAACAAACG 
GCATATGTCA ACCAAAATCA GAGACTCTCA GAGAGCCCTG CGCCAATGGA TGGCAAGCCC 
AGCTTAGCTA TAGACGACAG GCCAAAAATG GAGTCTGGTC CCATTAAGTC TATATCTCCT 
GGACCAAGAC CTTCAGAATC CTGTTTGGAG AAAAGAGAAA TCTCAAGTCT TCCATTACTA 
GTCTCTTCTT CTCCAGAGGT GGATGTTTCT TCCCATCCTA CAAGTGGATG CATCAAACCC 
ACTGCCGCAG GAGAACCAGA ATTTATCTCT CCTTCAGCCA CTAAGGCTCA GACGTATCAG 
GTCATCTCCG GTGAGGAATC TGTTCCTGAA GCTTCTCCCA GATTGTCTGC TTCATTGTCC 
CTCAGAGTGG TAAATGGTGT CAATGAGCCT CAAACTCCCT CAAGCTATGA AGAGCCTGAG 
GTCCAGGAAG CCCTAAAGAT GTCGTCATCC TGCGAGATTC AGGGTACATC ATTCATGGAG 
GAGTCTGGTC AGGAAGTTCC AGTGGCCTTG GAGGAGCTCC AGGCAGAGCA CCTCCCTTCT 
CTAGCTGCAC ATGTGCCCTT GATCCCAGGC GTACAGGCCT CTTCAATCAC CTCCAGCACC 
ACTTCTGTTC TTGCGCCACC ACCTGGGTTG GCACCATTAG TGCAGTCGGT TGTCCTTGAG 
GTGTCAGAAC AGAACAAGAC CTCAGACGAT GTCCAGCTGA AAGCTCAAGA TGCTTCAGAA 
TCACAAACTC AAAGCAACTC TAGAAAGTTT ACACCAGCCC AAGCTGTCCC TGTTGTACCA 
AAGCCTTGGA AGAAACCAAA CGACGAAGAT CTTATGATGG AAACGCTGAA GAAAGACAAT 
GAGGAGGAGA CCTCAAAGGA CTGCTCTGCT GGAGATGAGA TGGTCTTGCA GTCAAGCTTA 
GGGACCTACA GCAGCCCCTT TACAGTGCCT GAGCACCAGG AGAAGCACTC TGAAGAAAAC 
GGTGAGACCG AGACAGAGCC TTTCAGGAAC GGGGTTGAGA CGGAGAGCAC GGACAGTGGT 
GTGACGCTCG GGGACAGTAG GGGCTCCATG TGTTCCCCAA CTCCACTCAT TCAGGAAGGT 
CTTGTGCAAT CTAATGGAAA GAGACAGTAT GACCGGGACT TCCTGTTGGG CTTTCAGTTT 
ATGCCGGCCT GTGTGCAGAA GCCCGAGGGA CTGCCTCCAA TCAGTGATGT GGTGCTAGAC 
AAGATGAACC AAAACAAGCT GCCTCTGAGG TCTGTGGACC CCAGGTTGAT TTCCAGAGGG 
ACGCCTCAGG ATTTCACTCC TGCTTTTGCT GATTTCAGCA GGCAGATGGC TGGAGGACGC 
GGAGCCCCGC TTTTAAATGT GCGACGACTC CCACCACGCA AGATCATCGT AAACCTGTCT 
GTCAGTGATG ATGTTCAGCT CAAGAAGGCA GAAAATGCTT GGAAACCTGG GATGAAGAGA 
GAGGGTGTCG CAGAGGACCC AGAAACCATC AGAACCCAGG AGTTGTTCCG GAAAGTTCGT 
AGCATTTTAA ACAAGCTCAC GCCGCAGATG TTTCATCAGC TGATGAAGCA AGTGACGGAT 
CTTACTATCA ACACAGAGGA GAGGCTTAAA GGAGTCATCG ATCTTGTGTT TGAGAAGGCC 
ATCGATGAGC CCAGCTTCTC TGTGGCCTAC GCCAACATGT GTCGCTGCCT GGCCACGCTC 
AAAGTGCCCA CAGCAGACAA ACCTAATACA ACGGTGAACT TCCGGAAGCT CCTGTTGAAT 
CGCTGTCAAA AGGAATTCGA GAGGGACAAA GTAGATGACG TTGTCCTGGA GAGAAAACAA 
AAGGAGATTG ACTCTGCCAC ATCACCCACA GAAAAGGAAA GACTGCAGGA GGAGCTGGAG 
GAGGCGAAGG ACAAGGCCAG ACGTCGCTCC ACTGGAAATA TCAAATTCAT CGGTGAGCTG 
TTCAAGCTCA AGATGTTGAC AGAGCCCATC ATGCATGACT GCGTGGTGAA ACTGCTCAAA 
AACCACGATG ATGAGAGCTT AGAGTGCCTC TGCAGACTCC TCACCACCAT CGGCAAAGAC 
CTGGACTTCG AGAAGGCCAA GCCCCGAATG GACCAGTACT TCAACCAGAT GGAGAAAATT 
GTCAAAGAGC GCAAGACCTC GTCTCGGATA CGGTTCATGC TGCAGGATGT GATAGATCTG 
AGACTGCATA ACTGGGTTTC TCGGAGAGCT GACCAAGGCC CTAAAACCAT CGAGCAGATC 
CATAAAGAGG CCAAGCTGGA GGAGCAGGAG GAGCAGAGGA AAGTCCACCA GCAGCTTTTG 
TCTAAAGACA TGAAGAGGAG GCCAGATCAG AGAGAACAGC GTTCAATGAG AGAGGAGACT 
TGGAACACTG TGCCCACTAC CAAAAACAGC AGGACTATCG ACCCTACAAA GATTCCAAAG 
ATCTCCAAGC CGCAAATCGA TGAAAAGATT CAGCTGGGTC CGCGAGCTCA GATCAACTGG 
GTGAAAGGCA GCAGCGGCGG AGCAAAAGCC AGCGACTCTG AGTTGACCCG CTCTGCCGGA 
AACACTCTGA ACCGCTATTC AGCACTGCAG TCATCCACGT CTCAATCCAG TTCAACTCCG 
GCCCAAACCT CTGAATCTGA GTCCAGACGC TCGCTGGGAA GTCGAGGCAG TATGGGTCGC 
GAGCGCAGTG AAAAGCCTGT GAGTTTGGGG CCGTCACGAC CGGGTCCTTT TGGCAGAGGT 
AGCAGCGCTA AGGAGCTAAC AGATAACATG GTCCCAGAGG AAACACGGAG AGAAGGACAA 
GACTCACACC ACACATCTCG CAAAGCCAGC ATCTCAGAGG AGAAATGTGA GATGGAGAAC 
AGCAGATCCG CAGAGTCAGT GAAATCAGAG ACGCTGCTGG TCAGCGGTGT TTCAGAGAAG 
CCTGTCCTGT CTGAAGAGGA GATCGAGCGG AAGTCCAAAG CTATCATTGA TGAATTTCTG 
CACATTAATG ACTACAAGGA GGCAGTGCAG TGTGTGCTGG AGATAGAGCA GCCCTCTATG 
CTGTGTGTGT TTGTGCGCAT GGGTCTGGAG AGCACACTGG AGCGCAGTCA GAAAGCCAGA 
GAGCACATGG GACTGCTCTA CTATCAGCTT ATACAAAAGG GCATCTTACC CCATTCTCAA 
CTCTACAAAG GGTTTTCAGA GATGTTGGAG CAGGCTGATG ACATGGCCAT AGACATTCCC 
TTCATTTGGC TTTATCTGGC TGAGCTGCTC AGTCCTCTGT TGAAAGAGGG AGGAATCAAC 
ATGAGAGAGC TGTTCAGTGA GTTAAGTAAA CCGCTACTCC CTGTGGGAAG AGCTGCCATC 
TTATTGTCTG AAATACTGCA CCTACTATGC AAACAAATGA GCCATAGGAA AGTTGGAGAC 
TTGTGGAGAG AGTCTGGGCT GAACTGGGCC GACTTCCTGC CCGAGGGAGA ACATCTTCCT 
GCCTTCATCT CACAACATAA GTTAGAATTC ACAGTGTCGG ACATCTCTCC TGTCTCTCCG 
GCCTCAGCCA AACCCAGCCT CAGTCCTGAG GAGCTCTTTA AACAACTGGA GCAGCTCCTG 
CTAGAGGACA TGTCCAGCGA TGAACAGATT TTTGACTGGA TCGAGGCTAA TTTGGACGAA 
TCTCAGATGA GTTCTTCTCC TTTTTTGAGA GCTTTAATGA CTGCTATATG TAAAGCAGCA 
GTTAAAGATG AAAGCACGTC CTGTCGAGTG GACACTGCTA TCATCCAGAA ACGCCTGCCT 
ATATTACATA AGTACTTCGA CTCGGACACC GAGCGGCAGC TGCAAGCACT TTATGCTCTT 
CAGTCTCTGA TTGTCGCCCT GGATCAACCA CCCAACCTAC TCCGGATGTT TTTCGATTGT 
CTCTACGATG AGGACGTCAT TTCAGAGGAC GCTTTCTATC AATGGGAGAC GAGTAAAGAC 
CCGACAGAGC AGCTGGGAAA AGGCGTAGCG CTGAAGTCTG TGAACGCTTT CTTCACCTGG 
TTACGTGAAG CCGAGGAAGA ATCTGAGGAT AATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G3A ( XM_009306089.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304364.1 Danio rerio eukaryotic translation initiation factor 4 gamma, 3a (eif4g3a), transcript variant X1, mRNA.

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CloneID	ODa44791
Clone ID Related Accession (Same CDS sequence)	XM_009306089.2 , XM_009306089.3
Accession Version	XM_009306089.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	eukaryotic translation initiation factor 4 gamma 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306089.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTTGC CACCGAAAGT GGTTCCGAAA CCGGCCGCTG TCGCTGTTAG TGGACACGTA 
ACCGGACCGG CACCGCCAAC GCAGCTCCGC GCAGCGCTGA CCTCCGTGTC GTTGCCACCG 
GGAGCCCAAA ACGCTCCGCC GTCAGCCGTG CCGCCTACCC AGATTCCTAG AGCAGCGCTA 
TCACTGGATG AACGCATGTT TCCCGCTCAC TCTGGGGTGA CTGCGGTCTA CAGTGTATCC 
AGACACCCTG GTCCTCCATT TCCAGGTCAT GACCTCAGCA AAACCCACCC AAACCTGGCA 
GGGACTCCTC CTGGCCATGC CACGTCCCCG GCCCTCTCTC AGGTATCAGT TCCTGCTGGC 
CCTTCGTACC GCATCCTCAA GCCCTGGGAG ACAGGCGGAG CGCCTCCATA TAACCCAGCT 
CAGAATGCAG GCTCCGCCCC CCTGGTGTAC TCGCCACAGA CGCAGCCAAT GAACGTACAG 
CCACAGACTC GACCGTTTGT GACGGGACCT CGGCCAACAC ACCATCAGTT CATACACAGG 
AGTCAAATGC AGCCAGCGCG GCCTACCCTT CCGACTAACA ACCCCTCCAT TCGGCCGGGC 
TCACAGACCC CCACGGCGAC AGTTTATCCA CCAAACCAAC CCATCATGAT GACCATGACG 
CCCATGCCAT TTGCTACCCA GACACACCAA TATTACATTC CCCAGTATCG TCATAGTGCA 
CCTTACGTGG GACCCCCGCA GCAGTATGCC GTCCAGCCTC CAGGGTCTGG AACTTTCTAC 
CCTGGACCCA GCCCTGCGGA ATATCCCACC CCTTATGCTG CTGGTCCGCC ATATTACACC 
GGGCAGACGG TGTACCCACC CTCACCACCC ATCATAGTGC CTGCACCAAT GCCACCTCCA 
CCAACCAAGC GAGAAAAGAA ACCATCCTCC CAGATCCGGA TCCGAGACCC CAACCAGGGT 
GGAAAGGACA TCACAGAGGA AATCATGTTT GGGAGCCGCA ATCCTACACC TCCTGCCGGA 
CACCCAGCCT CCACCTTAAC CCCACCTGCA GGACGTCCCT CCTCGACTCC GACCCCACCT 
TCAGGACGCC TGTCCTCCAC ACCCACCCCT CCACAGCGGC CCAGCAACTG TCAAACCCCA 
GAACAAACGG CATATGTCAA CCAAAATCAG AGACTCTCAG AGAGCCCTGC GCCAATGGAT 
GGCAAGCCCA GCTTAGCTAT AGACGACAGG CCAAAAATGG AGTCTGGTCC CATTAAGTCT 
ATATCTCCTG GACCAAGACC TTCAGAATCC TGTTTGGAGA AAAGAGAAAT CTCAAGTCTT 
CCATTACTAG TCTCTTCTTC TCCAGAGGTG GATGTTTCTT CCCATCCTAC AAGTGGATGC 
ATCAAACCCA CTGCCGCAGG AGAACCAGAA TTTATCTCTC CTTCAGCCAC TAAGGCTCAG 
ACGTATCAGG TCATCTCCGG TGAGGAATCT GTTCCTGAAG CTTCTCCCAG ATTGTCTGCT 
TCATTGTCCC TCAGAGTGGT AAATGGTGTC AATGAGCCTC AAACTCCCTC AAGCTATGAA 
GAGCCTGAGG TCCAGGAAGC CCTAAAGATG TCGTCATCCT GCGAGATTCA GGGTACATCA 
TTCATGGAGG AGTCTGGTCA GGAAGTTCCA GTGGCCTTGG AGGAGCTCCA GGCAGAGCAC 
CTCCCTTCTC TAGCTGCACA TGTGCCCTTG ATCCCAGGCG TACAGGCCTC TTCAATCACC 
TCCAGCACCA CTTCTGTTCT TGCGCCACCA CCTGGGTTGG CACCATTAGT GCAGTCGGTT 
GTCCTTGAGG TGTCAGAACA GAACAAGACC TCAGACGATG TCCAGCTGAA AGCTCAAGAT 
GCTTCAGAAT CACAAACTCA AAGCAACTCT AGAAAGTTTA CACCAGCCCA AGCTGTCCCT 
GTTGTACCAA AGCCTTGGAA GAAACCAAAC GACGAAGATC TTATGATGGA AACGCTGAAG 
AAAGACAATG AGGAGGAGAC CTCAAAGGAC TGCTCTGCTG GAGATGAGAT GGTCTTGCAG 
TCAAGCTTAG GGACCTACAG CAGCCCCTTT ACAGTGCCTG AGCACCAGGA GAAGCACTCT 
GAAGAAAACG GTGAGACCGA GACAGAGCCT TTCAGGAACG GGGTTGAGAC GGAGAGCACG 
GACAGTGGTG TGACGCTCGG GGACAGTAGG GGCTCCATGT GTTCCCCAAC TCCACTCATT 
CAGGAAGGTC TTGTGCAATC TAATGGAAAG AGACAGTATG ACCGGGACTT CCTGTTGGGC 
TTTCAGTTTA TGCCGGCCTG TGTGCAGAAG CCCGAGGGAC TGCCTCCAAT CAGTGATGTG 
GTGCTAGACA AGATGAACCA AAACAAGCTG CCTCTGAGGT CTGTGGACCC CAGGTTGATT 
TCCAGAGGGA CGCCTCAGGA TTTCACTCCT GCTTTTGCTG ATTTCAGCAG GCAGATGGCT 
GGAGGACGCG GAGCCCCGCT TTTAAATGTG CGACGACTCC CACCACGCAA GATCATCGTA 
AACCTGTCTG TCAGTGATGA TGTTCAGCTC AAGAAGGCAG AAAATGCTTG GAAACCTGGG 
ATGAAGAGAG AGGGTGTCGC AGAGGACCCA GAAACCATCA GAACCCAGGA GTTGTTCCGG 
AAAGTTCGTA GCATTTTAAA CAAGCTCACG CCGCAGATGT TTCATCAGCT GATGAAGCAA 
GTGACGGATC TTACTATCAA CACAGAGGAG AGGCTTAAAG GAGTCATCGA TCTTGTGTTT 
GAGAAGGCCA TCGATGAGCC CAGCTTCTCT GTGGCCTACG CCAACATGTG TCGCTGCCTG 
GCCACGCTCA AAGTGCCCAC AGCAGACAAA CCTAATACAA CGGTGAACTT CCGGAAGCTC 
CTGTTGAATC GCTGTCAAAA GGAATTCGAG AGGGACAAAG TAGATGACGT TGTCCTGGAG 
AGAAAACAAA AGGAGATTGA CTCTGCCACA TCACCCACAG AAAAGGAAAG ACTGCAGGAG 
GAGCTGGAGG AGGCGAAGGA CAAGGCCAGA CGTCGCTCCA CTGGAAATAT CAAATTCATC 
GGTGAGCTGT TCAAGCTCAA GATGTTGACA GAGCCCATCA TGCATGACTG CGTGGTGAAA 
CTGCTCAAAA ACCACGATGA TGAGAGCTTA GAGTGCCTCT GCAGACTCCT CACCACCATC 
GGCAAAGACC TGGACTTCGA GAAGGCCAAG CCCCGAATGG ACCAGTACTT CAACCAGATG 
GAGAAAATTG TCAAAGAGCG CAAGACCTCG TCTCGGATAC GGTTCATGCT GCAGGATGTG 
ATAGATCTGA GACTGCATAA CTGGGTTTCT CGGAGAGCTG ACCAAGGCCC TAAAACCATC 
GAGCAGATCC ATAAAGAGGC CAAGCTGGAG GAGCAGGAGG AGCAGAGGAA AGTCCACCAG 
CAGCTTTTGT CTAAAGACAT GAAGAGGAGG CCAGATCAGA GAGAACAGCG TTCAATGAGA 
GAGGAGACTT GGAACACTGT GCCCACTACC AAAAACAGCA GGACTATCGA CCCTACAAAG 
ATTCCAAAGA TCTCCAAGCC GCAAATCGAT GAAAAGATTC AGCTGGGTCC GCGAGCTCAG 
ATCAACTGGG TGAAAGGCAG CAGCGGCGGA GCAAAAGCCA GCGACTCTGA GTTGACCCGC 
TCTGCCGGAA ACACTCTGAA CCGCTATTCA GCACTGCAGT CATCCACGTC TCAATCCAGT 
TCAACTCCGG CCCAAACCTC TGAATCTGAG TCCAGACGCT CGCTGGGAAG TCGAGGCAGT 
ATGGGTCGCG AGCGCAGTGA AAAGCCTGTG AGTTTGGGGC CGTCACGACC GGGTCCTTTT 
GGCAGAGGTA GCAGCGCTAA GGAGCTAACA GATAACATGG TCCCAGAGGA AACACGGAGA 
GAAGGACAAG ACTCACACCA CACATCTCGC AAAGCCAGCA TCTCAGAGGA GAAATGTGAG 
ATGGAGAACA GCAGATCCGC AGAGTCAGTG AAATCAGAGA CGCTGCTGGT CAGCGGTGTT 
TCAGAGAAGC CTGTCCTGTC TGAAGAGGAG ATCGAGCGGA AGTCCAAAGC TATCATTGAT 
GAATTTCTGC ACATTAATGA CTACAAGGAG GCAGTGCAGT GTGTGCTGGA GATAGAGCAG 
CCCTCTATGC TGTGTGTGTT TGTGCGCATG GGTCTGGAGA GCACACTGGA GCGCAGTCAG 
AAAGCCAGAG AGCACATGGG ACTGCTCTAC TATCAGCTTA TACAAAAGGG CATCTTACCC 
CATTCTCAAC TCTACAAAGG GTTTTCAGAG ATGTTGGAGC AGGCTGATGA CATGGCCATA 
GACATTCCCT TCATTTGGCT TTATCTGGCT GAGCTGCTCA GTCCTCTGTT GAAAGAGGGA 
GGAATCAACA TGAGAGAGCT GTTCAGTGAG TTAAGTAAAC CGCTACTCCC TGTGGGAAGA 
GCTGCCATCT TATTGTCTGA AATACTGCAC CTACTATGCA AACAAATGAG CCATAGGAAA 
GTTGGAGACT TGTGGAGAGA GTCTGGGCTG AACTGGGCCG ACTTCCTGCC CGAGGGAGAA 
CATCTTCCTG CCTTCATCTC ACAACATAAG TTAGAATTCA CAGTGTCGGA CATCTCTCCT 
GTCTCTCCGG CCTCAGCCAA ACCCAGCCTC AGTCCTGAGG AGCTCTTTAA ACAACTGGAG 
CAGCTCCTGC TAGAGGACAT GTCCAGCGAT GAACAGATTT TTGACTGGAT CGAGGCTAAT 
TTGGACGAAT CTCAGATGAG TTCTTCTCCT TTTTTGAGAG CTTTAATGAC TGCTATATGT 
AAAGCAGCAG TTAAAGATGA AAGCACGTCC TGTCGAGTGG ACACTGCTAT CATCCAGAAA 
CGCCTGCCTA TATTACATAA GTACTTCGAC TCGGACACCG AGCGGCAGCT GCAAGCACTT 
TATGCTCTTC AGTCTCTGAT TGTCGCCCTG GATCAACCAC CCAACCTACT CCGGATGTTT 
TTCGATTGTC TCTACGATGA GGACGTCATT TCAGAGGACG CTTTCTATCA ATGGGAGACG 
AGTAAAGACC CGACAGAGCA GCTGGGAAAA GGCGTAGCGC TGAAGTCTGT GAACGCTTTC 
TTCACCTGGT TACGTGAAGC CGAGGAAGAA TCTGAGGATA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304364.1
CDS	489..5633
Translation	MSLPPKVVPKPAAVAVSGHVTGPAPPTQLRAALTSVSLPPGAQNAPPSAVPPTQIPRAALSLDERMFPAHSGVTAVYSVSRHPGPPFPGHDLSKTHPNLAGTPPGHATSPALSQVSVPAGPSYRILKPWETGGAPPYNPAQNAGSAPLVYSPQTQPMNVQPQTRPFVTGPRPTHHQFIHRSQMQPARPTLPTNNPSIRPGSQTPTATVYPPNQPIMMTMTPMPFATQTHQYYIPQYRHSAPYVGPPQQYAVQPPGSGTFYPGPSPAEYPTPYAAGPPYYTGQTVYPPSPPIIVPAPMPPPPTKREKKPSSQIRIRDPNQGGKDITEEIMFGSRNPTPPAGHPASTLTPPAGRPSSTPTPPSGRLSSTPTPPQRPSNCQTPEQTAYVNQNQRLSESPAPMDGKPSLAIDDRPKMESGPIKSISPGPRPSESCLEKREISSLPLLVSSSPEVDVSSHPTSGCIKPTAAGEPEFISPSATKAQTYQVISGEESVPEASPRLSASLSLRVVNGVNEPQTPSSYEEPEVQEALKMSSSCEIQGTSFMEESGQEVPVALEELQAEHLPSLAAHVPLIPGVQASSITSSTTSVLAPPPGLAPLVQSVVLEVSEQNKTSDDVQLKAQDASESQTQSNSRKFTPAQAVPVVPKPWKKPNDEDLMMETLKKDNEEETSKDCSAGDEMVLQSSLGTYSSPFTVPEHQEKHSEENGETETEPFRNGVETESTDSGVTLGDSRGSMCSPTPLIQEGLVQSNGKRQYDRDFLLGFQFMPACVQKPEGLPPISDVVLDKMNQNKLPLRSVDPRLISRGTPQDFTPAFADFSRQMAGGRGAPLLNVRRLPPRKIIVNLSVSDDVQLKKAENAWKPGMKREGVAEDPETIRTQELFRKVRSILNKLTPQMFHQLMKQVTDLTINTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLATLKVPTADKPNTTVNFRKLLLNRCQKEFERDKVDDVVLERKQKEIDSATSPTEKERLQEELEEAKDKARRRSTGNIKFIGELFKLKMLTEPIMHDCVVKLLKNHDDESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLHNWVSRRADQGPKTIEQIHKEAKLEEQEEQRKVHQQLLSKDMKRRPDQREQRSMREETWNTVPTTKNSRTIDPTKIPKISKPQIDEKIQLGPRAQINWVKGSSGGAKASDSELTRSAGNTLNRYSALQSSTSQSSSTPAQTSESESRRSLGSRGSMGRERSEKPVSLGPSRPGPFGRGSSAKELTDNMVPEETRREGQDSHHTSRKASISEEKCEMENSRSAESVKSETLLVSGVSEKPVLSEEEIERKSKAIIDEFLHINDYKEAVQCVLEIEQPSMLCVFVRMGLESTLERSQKAREHMGLLYYQLIQKGILPHSQLYKGFSEMLEQADDMAIDIPFIWLYLAELLSPLLKEGGINMRELFSELSKPLLPVGRAAILLSEILHLLCKQMSHRKVGDLWRESGLNWADFLPEGEHLPAFISQHKLEFTVSDISPVSPASAKPSLSPEELFKQLEQLLLEDMSSDEQIFDWIEANLDESQMSSSPFLRALMTAICKAAVKDESTSCRVDTAIIQKRLPILHKYFDSDTERQLQALYALQSLIVALDQPPNLLRMFFDCLYDEDVISEDAFYQWETSKDPTEQLGKGVALKSVNAFFTWLREAEEESEDN

Target ORF information:

RefSeq Version	XM_009306089.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio eukaryotic translation initiation factor 4 gamma, 3a (eif4g3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306089.2

ORF Insert Sequence:

ATGAGTTTGC CACCGAAAGT GGTTCCGAAA CCGGCCGCTG TCGCTGTTAG TGGACACGTA 
ACCGGACCGG CACCGCCAAC GCAGCTCCGC GCAGCGCTGA CCTCCGTGTC GTTGCCACCG 
GGAGCCCAAA ACGCTCCGCC GTCAGCCGTG CCGCCTACCC AGATTCCTAG AGCAGCGCTA 
TCACTGGATG AACGCATGTT TCCCGCTCAC TCTGGGGTGA CTGCGGTCTA CAGTGTATCC 
AGACACCCTG GTCCTCCATT TCCAGGTCAT GACCTCAGCA AAACCCACCC AAACCTGGCA 
GGGACTCCTC CTGGCCATGC CACGTCCCCG GCCCTCTCTC AGGTATCAGT TCCTGCTGGC 
CCTTCGTACC GCATCCTCAA GCCCTGGGAG ACAGGCGGAG CGCCTCCATA TAACCCAGCT 
CAGAATGCAG GCTCCGCCCC CCTGGTGTAC TCGCCACAGA CGCAGCCAAT GAACGTACAG 
CCACAGACTC GACCGTTTGT GACGGGACCT CGGCCAACAC ACCATCAGTT CATACACAGG 
AGTCAAATGC AGCCAGCGCG GCCTACCCTT CCGACTAACA ACCCCTCCAT TCGGCCGGGC 
TCACAGACCC CCACGGCGAC AGTTTATCCA CCAAACCAAC CCATCATGAT GACCATGACG 
CCCATGCCAT TTGCTACCCA GACACACCAA TATTACATTC CCCAGTATCG TCATAGTGCA 
CCTTACGTGG GACCCCCGCA GCAGTATGCC GTCCAGCCTC CAGGGTCTGG AACTTTCTAC 
CCTGGACCCA GCCCTGCGGA ATATCCCACC CCTTATGCTG CTGGTCCGCC ATATTACACC 
GGGCAGACGG TGTACCCACC CTCACCACCC ATCATAGTGC CTGCACCAAT GCCACCTCCA 
CCAACCAAGC GAGAAAAGAA ACCATCCTCC CAGATCCGGA TCCGAGACCC CAACCAGGGT 
GGAAAGGACA TCACAGAGGA AATCATGTTT GGGAGCCGCA ATCCTACACC TCCTGCCGGA 
CACCCAGCCT CCACCTTAAC CCCACCTGCA GGACGTCCCT CCTCGACTCC GACCCCACCT 
TCAGGACGCC TGTCCTCCAC ACCCACCCCT CCACAGCGGC CCAGCAACTG TCAAACCCCA 
GAACAAACGG CATATGTCAA CCAAAATCAG AGACTCTCAG AGAGCCCTGC GCCAATGGAT 
GGCAAGCCCA GCTTAGCTAT AGACGACAGG CCAAAAATGG AGTCTGGTCC CATTAAGTCT 
ATATCTCCTG GACCAAGACC TTCAGAATCC TGTTTGGAGA AAAGAGAAAT CTCAAGTCTT 
CCATTACTAG TCTCTTCTTC TCCAGAGGTG GATGTTTCTT CCCATCCTAC AAGTGGATGC 
ATCAAACCCA CTGCCGCAGG AGAACCAGAA TTTATCTCTC CTTCAGCCAC TAAGGCTCAG 
ACGTATCAGG TCATCTCCGG TGAGGAATCT GTTCCTGAAG CTTCTCCCAG ATTGTCTGCT 
TCATTGTCCC TCAGAGTGGT AAATGGTGTC AATGAGCCTC AAACTCCCTC AAGCTATGAA 
GAGCCTGAGG TCCAGGAAGC CCTAAAGATG TCGTCATCCT GCGAGATTCA GGGTACATCA 
TTCATGGAGG AGTCTGGTCA GGAAGTTCCA GTGGCCTTGG AGGAGCTCCA GGCAGAGCAC 
CTCCCTTCTC TAGCTGCACA TGTGCCCTTG ATCCCAGGCG TACAGGCCTC TTCAATCACC 
TCCAGCACCA CTTCTGTTCT TGCGCCACCA CCTGGGTTGG CACCATTAGT GCAGTCGGTT 
GTCCTTGAGG TGTCAGAACA GAACAAGACC TCAGACGATG TCCAGCTGAA AGCTCAAGAT 
GCTTCAGAAT CACAAACTCA AAGCAACTCT AGAAAGTTTA CACCAGCCCA AGCTGTCCCT 
GTTGTACCAA AGCCTTGGAA GAAACCAAAC GACGAAGATC TTATGATGGA AACGCTGAAG 
AAAGACAATG AGGAGGAGAC CTCAAAGGAC TGCTCTGCTG GAGATGAGAT GGTCTTGCAG 
TCAAGCTTAG GGACCTACAG CAGCCCCTTT ACAGTGCCTG AGCACCAGGA GAAGCACTCT 
GAAGAAAACG GTGAGACCGA GACAGAGCCT TTCAGGAACG GGGTTGAGAC GGAGAGCACG 
GACAGTGGTG TGACGCTCGG GGACAGTAGG GGCTCCATGT GTTCCCCAAC TCCACTCATT 
CAGGAAGGTC TTGTGCAATC TAATGGAAAG AGACAGTATG ACCGGGACTT CCTGTTGGGC 
TTTCAGTTTA TGCCGGCCTG TGTGCAGAAG CCCGAGGGAC TGCCTCCAAT CAGTGATGTG 
GTGCTAGACA AGATGAACCA AAACAAGCTG CCTCTGAGGT CTGTGGACCC CAGGTTGATT 
TCCAGAGGGA CGCCTCAGGA TTTCACTCCT GCTTTTGCTG ATTTCAGCAG GCAGATGGCT 
GGAGGACGCG GAGCCCCGCT TTTAAATGTG CGACGACTCC CACCACGCAA GATCATCGTA 
AACCTGTCTG TCAGTGATGA TGTTCAGCTC AAGAAGGCAG AAAATGCTTG GAAACCTGGG 
ATGAAGAGAG AGGGTGTCGC AGAGGACCCA GAAACCATCA GAACCCAGGA GTTGTTCCGG 
AAAGTTCGTA GCATTTTAAA CAAGCTCACG CCGCAGATGT TTCATCAGCT GATGAAGCAA 
GTGACGGATC TTACTATCAA CACAGAGGAG AGGCTTAAAG GAGTCATCGA TCTTGTGTTT 
GAGAAGGCCA TCGATGAGCC CAGCTTCTCT GTGGCCTACG CCAACATGTG TCGCTGCCTG 
GCCACGCTCA AAGTGCCCAC AGCAGACAAA CCTAATACAA CGGTGAACTT CCGGAAGCTC 
CTGTTGAATC GCTGTCAAAA GGAATTCGAG AGGGACAAAG TAGATGACGT TGTCCTGGAG 
AGAAAACAAA AGGAGATTGA CTCTGCCACA TCACCCACAG AAAAGGAAAG ACTGCAGGAG 
GAGCTGGAGG AGGCGAAGGA CAAGGCCAGA CGTCGCTCCA CTGGAAATAT CAAATTCATC 
GGTGAGCTGT TCAAGCTCAA GATGTTGACA GAGCCCATCA TGCATGACTG CGTGGTGAAA 
CTGCTCAAAA ACCACGATGA TGAGAGCTTA GAGTGCCTCT GCAGACTCCT CACCACCATC 
GGCAAAGACC TGGACTTCGA GAAGGCCAAG CCCCGAATGG ACCAGTACTT CAACCAGATG 
GAGAAAATTG TCAAAGAGCG CAAGACCTCG TCTCGGATAC GGTTCATGCT GCAGGATGTG 
ATAGATCTGA GACTGCATAA CTGGGTTTCT CGGAGAGCTG ACCAAGGCCC TAAAACCATC 
GAGCAGATCC ATAAAGAGGC CAAGCTGGAG GAGCAGGAGG AGCAGAGGAA AGTCCACCAG 
CAGCTTTTGT CTAAAGACAT GAAGAGGAGG CCAGATCAGA GAGAACAGCG TTCAATGAGA 
GAGGAGACTT GGAACACTGT GCCCACTACC AAAAACAGCA GGACTATCGA CCCTACAAAG 
ATTCCAAAGA TCTCCAAGCC GCAAATCGAT GAAAAGATTC AGCTGGGTCC GCGAGCTCAG 
ATCAACTGGG TGAAAGGCAG CAGCGGCGGA GCAAAAGCCA GCGACTCTGA GTTGACCCGC 
TCTGCCGGAA ACACTCTGAA CCGCTATTCA GCACTGCAGT CATCCACGTC TCAATCCAGT 
TCAACTCCGG CCCAAACCTC TGAATCTGAG TCCAGACGCT CGCTGGGAAG TCGAGGCAGT 
ATGGGTCGCG AGCGCAGTGA AAAGCCTGTG AGTTTGGGGC CGTCACGACC GGGTCCTTTT 
GGCAGAGGTA GCAGCGCTAA GGAGCTAACA GATAACATGG TCCCAGAGGA AACACGGAGA 
GAAGGACAAG ACTCACACCA CACATCTCGC AAAGCCAGCA TCTCAGAGGA GAAATGTGAG 
ATGGAGAACA GCAGATCCGC AGAGTCAGTG AAATCAGAGA CGCTGCTGGT CAGCGGTGTT 
TCAGAGAAGC CTGTCCTGTC TGAAGAGGAG ATCGAGCGGA AGTCCAAAGC TATCATTGAT 
GAATTTCTGC ACATTAATGA CTACAAGGAG GCAGTGCAGT GTGTGCTGGA GATAGAGCAG 
CCCTCTATGC TGTGTGTGTT TGTGCGCATG GGTCTGGAGA GCACACTGGA GCGCAGTCAG 
AAAGCCAGAG AGCACATGGG ACTGCTCTAC TATCAGCTTA TACAAAAGGG CATCTTACCC 
CATTCTCAAC TCTACAAAGG GTTTTCAGAG ATGTTGGAGC AGGCTGATGA CATGGCCATA 
GACATTCCCT TCATTTGGCT TTATCTGGCT GAGCTGCTCA GTCCTCTGTT GAAAGAGGGA 
GGAATCAACA TGAGAGAGCT GTTCAGTGAG TTAAGTAAAC CGCTACTCCC TGTGGGAAGA 
GCTGCCATCT TATTGTCTGA AATACTGCAC CTACTATGCA AACAAATGAG CCATAGGAAA 
GTTGGAGACT TGTGGAGAGA GTCTGGGCTG AACTGGGCCG ACTTCCTGCC CGAGGGAGAA 
CATCTTCCTG CCTTCATCTC ACAACATAAG TTAGAATTCA CAGTGTCGGA CATCTCTCCT 
GTCTCTCCGG CCTCAGCCAA ACCCAGCCTC AGTCCTGAGG AGCTCTTTAA ACAACTGGAG 
CAGCTCCTGC TAGAGGACAT GTCCAGCGAT GAACAGATTT TTGACTGGAT CGAGGCTAAT 
TTGGACGAAT CTCAGATGAG TTCTTCTCCT TTTTTGAGAG CTTTAATGAC TGCTATATGT 
AAAGCAGCAG TTAAAGATGA AAGCACGTCC TGTCGAGTGG ACACTGCTAT CATCCAGAAA 
CGCCTGCCTA TATTACATAA GTACTTCGAC TCGGACACCG AGCGGCAGCT GCAAGCACTT 
TATGCTCTTC AGTCTCTGAT TGTCGCCCTG GATCAACCAC CCAACCTACT CCGGATGTTT 
TTCGATTGTC TCTACGATGA GGACGTCATT TCAGAGGACG CTTTCTATCA ATGGGAGACG 
AGTAAAGACC CGACAGAGCA GCTGGGAAAA GGCGTAGCGC TGAAGTCTGT GAACGCTTTC 
TTCACCTGGT TACGTGAAGC CGAGGAAGAA TCTGAGGATA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G3A ( XM_009306089.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304364.1 Danio rerio eukaryotic translation initiation factor 4 gamma, 3a (eif4g3a), transcript variant X1, mRNA.

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CloneID	ODa44791
Clone ID Related Accession (Same CDS sequence)	XM_009306089.2 , XM_009306089.3
Accession Version	XM_009306089.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	eukaryotic translation initiation factor 4 gamma 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306089.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTTGC CACCGAAAGT GGTTCCGAAA CCGGCCGCTG TCGCTGTTAG TGGACACGTA 
ACCGGACCGG CACCGCCAAC GCAGCTCCGC GCAGCGCTGA CCTCCGTGTC GTTGCCACCG 
GGAGCCCAAA ACGCTCCGCC GTCAGCCGTG CCGCCTACCC AGATTCCTAG AGCAGCGCTA 
TCACTGGATG AACGCATGTT TCCCGCTCAC TCTGGGGTGA CTGCGGTCTA CAGTGTATCC 
AGACACCCTG GTCCTCCATT TCCAGGTCAT GACCTCAGCA AAACCCACCC AAACCTGGCA 
GGGACTCCTC CTGGCCATGC CACGTCCCCG GCCCTCTCTC AGGTATCAGT TCCTGCTGGC 
CCTTCGTACC GCATCCTCAA GCCCTGGGAG ACAGGCGGAG CGCCTCCATA TAACCCAGCT 
CAGAATGCAG GCTCCGCCCC CCTGGTGTAC TCGCCACAGA CGCAGCCAAT GAACGTACAG 
CCACAGACTC GACCGTTTGT GACGGGACCT CGGCCAACAC ACCATCAGTT CATACACAGG 
AGTCAAATGC AGCCAGCGCG GCCTACCCTT CCGACTAACA ACCCCTCCAT TCGGCCGGGC 
TCACAGACCC CCACGGCGAC AGTTTATCCA CCAAACCAAC CCATCATGAT GACCATGACG 
CCCATGCCAT TTGCTACCCA GACACACCAA TATTACATTC CCCAGTATCG TCATAGTGCA 
CCTTACGTGG GACCCCCGCA GCAGTATGCC GTCCAGCCTC CAGGGTCTGG AACTTTCTAC 
CCTGGACCCA GCCCTGCGGA ATATCCCACC CCTTATGCTG CTGGTCCGCC ATATTACACC 
GGGCAGACGG TGTACCCACC CTCACCACCC ATCATAGTGC CTGCACCAAT GCCACCTCCA 
CCAACCAAGC GAGAAAAGAA ACCATCCTCC CAGATCCGGA TCCGAGACCC CAACCAGGGT 
GGAAAGGACA TCACAGAGGA AATCATGTTT GGGAGCCGCA ATCCTACACC TCCTGCCGGA 
CACCCAGCCT CCACCTTAAC CCCACCTGCA GGACGTCCCT CCTCGACTCC GACCCCACCT 
TCAGGACGCC TGTCCTCCAC ACCCACCCCT CCACAGCGGC CCAGCAACTG TCAAACCCCA 
GAACAAACGG CATATGTCAA CCAAAATCAG AGACTCTCAG AGAGCCCTGC GCCAATGGAT 
GGCAAGCCCA GCTTAGCTAT AGACGACAGG CCAAAAATGG AGTCTGGTCC CATTAAGTCT 
ATATCTCCTG GACCAAGACC TTCAGAATCC TGTTTGGAGA AAAGAGAAAT CTCAAGTCTT 
CCATTACTAG TCTCTTCTTC TCCAGAGGTG GATGTTTCTT CCCATCCTAC AAGTGGATGC 
ATCAAACCCA CTGCCGCAGG AGAACCAGAA TTTATCTCTC CTTCAGCCAC TAAGGCTCAG 
ACGTATCAGG TCATCTCCGG TGAGGAATCT GTTCCTGAAG CTTCTCCCAG ATTGTCTGCT 
TCATTGTCCC TCAGAGTGGT AAATGGTGTC AATGAGCCTC AAACTCCCTC AAGCTATGAA 
GAGCCTGAGG TCCAGGAAGC CCTAAAGATG TCGTCATCCT GCGAGATTCA GGGTACATCA 
TTCATGGAGG AGTCTGGTCA GGAAGTTCCA GTGGCCTTGG AGGAGCTCCA GGCAGAGCAC 
CTCCCTTCTC TAGCTGCACA TGTGCCCTTG ATCCCAGGCG TACAGGCCTC TTCAATCACC 
TCCAGCACCA CTTCTGTTCT TGCGCCACCA CCTGGGTTGG CACCATTAGT GCAGTCGGTT 
GTCCTTGAGG TGTCAGAACA GAACAAGACC TCAGACGATG TCCAGCTGAA AGCTCAAGAT 
GCTTCAGAAT CACAAACTCA AAGCAACTCT AGAAAGTTTA CACCAGCCCA AGCTGTCCCT 
GTTGTACCAA AGCCTTGGAA GAAACCAAAC GACGAAGATC TTATGATGGA AACGCTGAAG 
AAAGACAATG AGGAGGAGAC CTCAAAGGAC TGCTCTGCTG GAGATGAGAT GGTCTTGCAG 
TCAAGCTTAG GGACCTACAG CAGCCCCTTT ACAGTGCCTG AGCACCAGGA GAAGCACTCT 
GAAGAAAACG GTGAGACCGA GACAGAGCCT TTCAGGAACG GGGTTGAGAC GGAGAGCACG 
GACAGTGGTG TGACGCTCGG GGACAGTAGG GGCTCCATGT GTTCCCCAAC TCCACTCATT 
CAGGAAGGTC TTGTGCAATC TAATGGAAAG AGACAGTATG ACCGGGACTT CCTGTTGGGC 
TTTCAGTTTA TGCCGGCCTG TGTGCAGAAG CCCGAGGGAC TGCCTCCAAT CAGTGATGTG 
GTGCTAGACA AGATGAACCA AAACAAGCTG CCTCTGAGGT CTGTGGACCC CAGGTTGATT 
TCCAGAGGGA CGCCTCAGGA TTTCACTCCT GCTTTTGCTG ATTTCAGCAG GCAGATGGCT 
GGAGGACGCG GAGCCCCGCT TTTAAATGTG CGACGACTCC CACCACGCAA GATCATCGTA 
AACCTGTCTG TCAGTGATGA TGTTCAGCTC AAGAAGGCAG AAAATGCTTG GAAACCTGGG 
ATGAAGAGAG AGGGTGTCGC AGAGGACCCA GAAACCATCA GAACCCAGGA GTTGTTCCGG 
AAAGTTCGTA GCATTTTAAA CAAGCTCACG CCGCAGATGT TTCATCAGCT GATGAAGCAA 
GTGACGGATC TTACTATCAA CACAGAGGAG AGGCTTAAAG GAGTCATCGA TCTTGTGTTT 
GAGAAGGCCA TCGATGAGCC CAGCTTCTCT GTGGCCTACG CCAACATGTG TCGCTGCCTG 
GCCACGCTCA AAGTGCCCAC AGCAGACAAA CCTAATACAA CGGTGAACTT CCGGAAGCTC 
CTGTTGAATC GCTGTCAAAA GGAATTCGAG AGGGACAAAG TAGATGACGT TGTCCTGGAG 
AGAAAACAAA AGGAGATTGA CTCTGCCACA TCACCCACAG AAAAGGAAAG ACTGCAGGAG 
GAGCTGGAGG AGGCGAAGGA CAAGGCCAGA CGTCGCTCCA CTGGAAATAT CAAATTCATC 
GGTGAGCTGT TCAAGCTCAA GATGTTGACA GAGCCCATCA TGCATGACTG CGTGGTGAAA 
CTGCTCAAAA ACCACGATGA TGAGAGCTTA GAGTGCCTCT GCAGACTCCT CACCACCATC 
GGCAAAGACC TGGACTTCGA GAAGGCCAAG CCCCGAATGG ACCAGTACTT CAACCAGATG 
GAGAAAATTG TCAAAGAGCG CAAGACCTCG TCTCGGATAC GGTTCATGCT GCAGGATGTG 
ATAGATCTGA GACTGCATAA CTGGGTTTCT CGGAGAGCTG ACCAAGGCCC TAAAACCATC 
GAGCAGATCC ATAAAGAGGC CAAGCTGGAG GAGCAGGAGG AGCAGAGGAA AGTCCACCAG 
CAGCTTTTGT CTAAAGACAT GAAGAGGAGG CCAGATCAGA GAGAACAGCG TTCAATGAGA 
GAGGAGACTT GGAACACTGT GCCCACTACC AAAAACAGCA GGACTATCGA CCCTACAAAG 
ATTCCAAAGA TCTCCAAGCC GCAAATCGAT GAAAAGATTC AGCTGGGTCC GCGAGCTCAG 
ATCAACTGGG TGAAAGGCAG CAGCGGCGGA GCAAAAGCCA GCGACTCTGA GTTGACCCGC 
TCTGCCGGAA ACACTCTGAA CCGCTATTCA GCACTGCAGT CATCCACGTC TCAATCCAGT 
TCAACTCCGG CCCAAACCTC TGAATCTGAG TCCAGACGCT CGCTGGGAAG TCGAGGCAGT 
ATGGGTCGCG AGCGCAGTGA AAAGCCTGTG AGTTTGGGGC CGTCACGACC GGGTCCTTTT 
GGCAGAGGTA GCAGCGCTAA GGAGCTAACA GATAACATGG TCCCAGAGGA AACACGGAGA 
GAAGGACAAG ACTCACACCA CACATCTCGC AAAGCCAGCA TCTCAGAGGA GAAATGTGAG 
ATGGAGAACA GCAGATCCGC AGAGTCAGTG AAATCAGAGA CGCTGCTGGT CAGCGGTGTT 
TCAGAGAAGC CTGTCCTGTC TGAAGAGGAG ATCGAGCGGA AGTCCAAAGC TATCATTGAT 
GAATTTCTGC ACATTAATGA CTACAAGGAG GCAGTGCAGT GTGTGCTGGA GATAGAGCAG 
CCCTCTATGC TGTGTGTGTT TGTGCGCATG GGTCTGGAGA GCACACTGGA GCGCAGTCAG 
AAAGCCAGAG AGCACATGGG ACTGCTCTAC TATCAGCTTA TACAAAAGGG CATCTTACCC 
CATTCTCAAC TCTACAAAGG GTTTTCAGAG ATGTTGGAGC AGGCTGATGA CATGGCCATA 
GACATTCCCT TCATTTGGCT TTATCTGGCT GAGCTGCTCA GTCCTCTGTT GAAAGAGGGA 
GGAATCAACA TGAGAGAGCT GTTCAGTGAG TTAAGTAAAC CGCTACTCCC TGTGGGAAGA 
GCTGCCATCT TATTGTCTGA AATACTGCAC CTACTATGCA AACAAATGAG CCATAGGAAA 
GTTGGAGACT TGTGGAGAGA GTCTGGGCTG AACTGGGCCG ACTTCCTGCC CGAGGGAGAA 
CATCTTCCTG CCTTCATCTC ACAACATAAG TTAGAATTCA CAGTGTCGGA CATCTCTCCT 
GTCTCTCCGG CCTCAGCCAA ACCCAGCCTC AGTCCTGAGG AGCTCTTTAA ACAACTGGAG 
CAGCTCCTGC TAGAGGACAT GTCCAGCGAT GAACAGATTT TTGACTGGAT CGAGGCTAAT 
TTGGACGAAT CTCAGATGAG TTCTTCTCCT TTTTTGAGAG CTTTAATGAC TGCTATATGT 
AAAGCAGCAG TTAAAGATGA AAGCACGTCC TGTCGAGTGG ACACTGCTAT CATCCAGAAA 
CGCCTGCCTA TATTACATAA GTACTTCGAC TCGGACACCG AGCGGCAGCT GCAAGCACTT 
TATGCTCTTC AGTCTCTGAT TGTCGCCCTG GATCAACCAC CCAACCTACT CCGGATGTTT 
TTCGATTGTC TCTACGATGA GGACGTCATT TCAGAGGACG CTTTCTATCA ATGGGAGACG 
AGTAAAGACC CGACAGAGCA GCTGGGAAAA GGCGTAGCGC TGAAGTCTGT GAACGCTTTC 
TTCACCTGGT TACGTGAAGC CGAGGAAGAA TCTGAGGATA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304364.1
CDS	460..5604
Translation	MSLPPKVVPKPAAVAVSGHVTGPAPPTQLRAALTSVSLPPGAQNAPPSAVPPTQIPRAALSLDERMFPAHSGVTAVYSVSRHPGPPFPGHDLSKTHPNLAGTPPGHATSPALSQVSVPAGPSYRILKPWETGGAPPYNPAQNAGSAPLVYSPQTQPMNVQPQTRPFVTGPRPTHHQFIHRSQMQPARPTLPTNNPSIRPGSQTPTATVYPPNQPIMMTMTPMPFATQTHQYYIPQYRHSAPYVGPPQQYAVQPPGSGTFYPGPSPAEYPTPYAAGPPYYTGQTVYPPSPPIIVPAPMPPPPTKREKKPSSQIRIRDPNQGGKDITEEIMFGSRNPTPPAGHPASTLTPPAGRPSSTPTPPSGRLSSTPTPPQRPSNCQTPEQTAYVNQNQRLSESPAPMDGKPSLAIDDRPKMESGPIKSISPGPRPSESCLEKREISSLPLLVSSSPEVDVSSHPTSGCIKPTAAGEPEFISPSATKAQTYQVISGEESVPEASPRLSASLSLRVVNGVNEPQTPSSYEEPEVQEALKMSSSCEIQGTSFMEESGQEVPVALEELQAEHLPSLAAHVPLIPGVQASSITSSTTSVLAPPPGLAPLVQSVVLEVSEQNKTSDDVQLKAQDASESQTQSNSRKFTPAQAVPVVPKPWKKPNDEDLMMETLKKDNEEETSKDCSAGDEMVLQSSLGTYSSPFTVPEHQEKHSEENGETETEPFRNGVETESTDSGVTLGDSRGSMCSPTPLIQEGLVQSNGKRQYDRDFLLGFQFMPACVQKPEGLPPISDVVLDKMNQNKLPLRSVDPRLISRGTPQDFTPAFADFSRQMAGGRGAPLLNVRRLPPRKIIVNLSVSDDVQLKKAENAWKPGMKREGVAEDPETIRTQELFRKVRSILNKLTPQMFHQLMKQVTDLTINTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLATLKVPTADKPNTTVNFRKLLLNRCQKEFERDKVDDVVLERKQKEIDSATSPTEKERLQEELEEAKDKARRRSTGNIKFIGELFKLKMLTEPIMHDCVVKLLKNHDDESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLHNWVSRRADQGPKTIEQIHKEAKLEEQEEQRKVHQQLLSKDMKRRPDQREQRSMREETWNTVPTTKNSRTIDPTKIPKISKPQIDEKIQLGPRAQINWVKGSSGGAKASDSELTRSAGNTLNRYSALQSSTSQSSSTPAQTSESESRRSLGSRGSMGRERSEKPVSLGPSRPGPFGRGSSAKELTDNMVPEETRREGQDSHHTSRKASISEEKCEMENSRSAESVKSETLLVSGVSEKPVLSEEEIERKSKAIIDEFLHINDYKEAVQCVLEIEQPSMLCVFVRMGLESTLERSQKAREHMGLLYYQLIQKGILPHSQLYKGFSEMLEQADDMAIDIPFIWLYLAELLSPLLKEGGINMRELFSELSKPLLPVGRAAILLSEILHLLCKQMSHRKVGDLWRESGLNWADFLPEGEHLPAFISQHKLEFTVSDISPVSPASAKPSLSPEELFKQLEQLLLEDMSSDEQIFDWIEANLDESQMSSSPFLRALMTAICKAAVKDESTSCRVDTAIIQKRLPILHKYFDSDTERQLQALYALQSLIVALDQPPNLLRMFFDCLYDEDVISEDAFYQWETSKDPTEQLGKGVALKSVNAFFTWLREAEEESEDN

Target ORF information:

RefSeq Version	XM_009306089.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio eukaryotic translation initiation factor 4 gamma, 3a (eif4g3a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306089.3

ORF Insert Sequence:

ATGAGTTTGC CACCGAAAGT GGTTCCGAAA CCGGCCGCTG TCGCTGTTAG TGGACACGTA 
ACCGGACCGG CACCGCCAAC GCAGCTCCGC GCAGCGCTGA CCTCCGTGTC GTTGCCACCG 
GGAGCCCAAA ACGCTCCGCC GTCAGCCGTG CCGCCTACCC AGATTCCTAG AGCAGCGCTA 
TCACTGGATG AACGCATGTT TCCCGCTCAC TCTGGGGTGA CTGCGGTCTA CAGTGTATCC 
AGACACCCTG GTCCTCCATT TCCAGGTCAT GACCTCAGCA AAACCCACCC AAACCTGGCA 
GGGACTCCTC CTGGCCATGC CACGTCCCCG GCCCTCTCTC AGGTATCAGT TCCTGCTGGC 
CCTTCGTACC GCATCCTCAA GCCCTGGGAG ACAGGCGGAG CGCCTCCATA TAACCCAGCT 
CAGAATGCAG GCTCCGCCCC CCTGGTGTAC TCGCCACAGA CGCAGCCAAT GAACGTACAG 
CCACAGACTC GACCGTTTGT GACGGGACCT CGGCCAACAC ACCATCAGTT CATACACAGG 
AGTCAAATGC AGCCAGCGCG GCCTACCCTT CCGACTAACA ACCCCTCCAT TCGGCCGGGC 
TCACAGACCC CCACGGCGAC AGTTTATCCA CCAAACCAAC CCATCATGAT GACCATGACG 
CCCATGCCAT TTGCTACCCA GACACACCAA TATTACATTC CCCAGTATCG TCATAGTGCA 
CCTTACGTGG GACCCCCGCA GCAGTATGCC GTCCAGCCTC CAGGGTCTGG AACTTTCTAC 
CCTGGACCCA GCCCTGCGGA ATATCCCACC CCTTATGCTG CTGGTCCGCC ATATTACACC 
GGGCAGACGG TGTACCCACC CTCACCACCC ATCATAGTGC CTGCACCAAT GCCACCTCCA 
CCAACCAAGC GAGAAAAGAA ACCATCCTCC CAGATCCGGA TCCGAGACCC CAACCAGGGT 
GGAAAGGACA TCACAGAGGA AATCATGTTT GGGAGCCGCA ATCCTACACC TCCTGCCGGA 
CACCCAGCCT CCACCTTAAC CCCACCTGCA GGACGTCCCT CCTCGACTCC GACCCCACCT 
TCAGGACGCC TGTCCTCCAC ACCCACCCCT CCACAGCGGC CCAGCAACTG TCAAACCCCA 
GAACAAACGG CATATGTCAA CCAAAATCAG AGACTCTCAG AGAGCCCTGC GCCAATGGAT 
GGCAAGCCCA GCTTAGCTAT AGACGACAGG CCAAAAATGG AGTCTGGTCC CATTAAGTCT 
ATATCTCCTG GACCAAGACC TTCAGAATCC TGTTTGGAGA AAAGAGAAAT CTCAAGTCTT 
CCATTACTAG TCTCTTCTTC TCCAGAGGTG GATGTTTCTT CCCATCCTAC AAGTGGATGC 
ATCAAACCCA CTGCCGCAGG AGAACCAGAA TTTATCTCTC CTTCAGCCAC TAAGGCTCAG 
ACGTATCAGG TCATCTCCGG TGAGGAATCT GTTCCTGAAG CTTCTCCCAG ATTGTCTGCT 
TCATTGTCCC TCAGAGTGGT AAATGGTGTC AATGAGCCTC AAACTCCCTC AAGCTATGAA 
GAGCCTGAGG TCCAGGAAGC CCTAAAGATG TCGTCATCCT GCGAGATTCA GGGTACATCA 
TTCATGGAGG AGTCTGGTCA GGAAGTTCCA GTGGCCTTGG AGGAGCTCCA GGCAGAGCAC 
CTCCCTTCTC TAGCTGCACA TGTGCCCTTG ATCCCAGGCG TACAGGCCTC TTCAATCACC 
TCCAGCACCA CTTCTGTTCT TGCGCCACCA CCTGGGTTGG CACCATTAGT GCAGTCGGTT 
GTCCTTGAGG TGTCAGAACA GAACAAGACC TCAGACGATG TCCAGCTGAA AGCTCAAGAT 
GCTTCAGAAT CACAAACTCA AAGCAACTCT AGAAAGTTTA CACCAGCCCA AGCTGTCCCT 
GTTGTACCAA AGCCTTGGAA GAAACCAAAC GACGAAGATC TTATGATGGA AACGCTGAAG 
AAAGACAATG AGGAGGAGAC CTCAAAGGAC TGCTCTGCTG GAGATGAGAT GGTCTTGCAG 
TCAAGCTTAG GGACCTACAG CAGCCCCTTT ACAGTGCCTG AGCACCAGGA GAAGCACTCT 
GAAGAAAACG GTGAGACCGA GACAGAGCCT TTCAGGAACG GGGTTGAGAC GGAGAGCACG 
GACAGTGGTG TGACGCTCGG GGACAGTAGG GGCTCCATGT GTTCCCCAAC TCCACTCATT 
CAGGAAGGTC TTGTGCAATC TAATGGAAAG AGACAGTATG ACCGGGACTT CCTGTTGGGC 
TTTCAGTTTA TGCCGGCCTG TGTGCAGAAG CCCGAGGGAC TGCCTCCAAT CAGTGATGTG 
GTGCTAGACA AGATGAACCA AAACAAGCTG CCTCTGAGGT CTGTGGACCC CAGGTTGATT 
TCCAGAGGGA CGCCTCAGGA TTTCACTCCT GCTTTTGCTG ATTTCAGCAG GCAGATGGCT 
GGAGGACGCG GAGCCCCGCT TTTAAATGTG CGACGACTCC CACCACGCAA GATCATCGTA 
AACCTGTCTG TCAGTGATGA TGTTCAGCTC AAGAAGGCAG AAAATGCTTG GAAACCTGGG 
ATGAAGAGAG AGGGTGTCGC AGAGGACCCA GAAACCATCA GAACCCAGGA GTTGTTCCGG 
AAAGTTCGTA GCATTTTAAA CAAGCTCACG CCGCAGATGT TTCATCAGCT GATGAAGCAA 
GTGACGGATC TTACTATCAA CACAGAGGAG AGGCTTAAAG GAGTCATCGA TCTTGTGTTT 
GAGAAGGCCA TCGATGAGCC CAGCTTCTCT GTGGCCTACG CCAACATGTG TCGCTGCCTG 
GCCACGCTCA AAGTGCCCAC AGCAGACAAA CCTAATACAA CGGTGAACTT CCGGAAGCTC 
CTGTTGAATC GCTGTCAAAA GGAATTCGAG AGGGACAAAG TAGATGACGT TGTCCTGGAG 
AGAAAACAAA AGGAGATTGA CTCTGCCACA TCACCCACAG AAAAGGAAAG ACTGCAGGAG 
GAGCTGGAGG AGGCGAAGGA CAAGGCCAGA CGTCGCTCCA CTGGAAATAT CAAATTCATC 
GGTGAGCTGT TCAAGCTCAA GATGTTGACA GAGCCCATCA TGCATGACTG CGTGGTGAAA 
CTGCTCAAAA ACCACGATGA TGAGAGCTTA GAGTGCCTCT GCAGACTCCT CACCACCATC 
GGCAAAGACC TGGACTTCGA GAAGGCCAAG CCCCGAATGG ACCAGTACTT CAACCAGATG 
GAGAAAATTG TCAAAGAGCG CAAGACCTCG TCTCGGATAC GGTTCATGCT GCAGGATGTG 
ATAGATCTGA GACTGCATAA CTGGGTTTCT CGGAGAGCTG ACCAAGGCCC TAAAACCATC 
GAGCAGATCC ATAAAGAGGC CAAGCTGGAG GAGCAGGAGG AGCAGAGGAA AGTCCACCAG 
CAGCTTTTGT CTAAAGACAT GAAGAGGAGG CCAGATCAGA GAGAACAGCG TTCAATGAGA 
GAGGAGACTT GGAACACTGT GCCCACTACC AAAAACAGCA GGACTATCGA CCCTACAAAG 
ATTCCAAAGA TCTCCAAGCC GCAAATCGAT GAAAAGATTC AGCTGGGTCC GCGAGCTCAG 
ATCAACTGGG TGAAAGGCAG CAGCGGCGGA GCAAAAGCCA GCGACTCTGA GTTGACCCGC 
TCTGCCGGAA ACACTCTGAA CCGCTATTCA GCACTGCAGT CATCCACGTC TCAATCCAGT 
TCAACTCCGG CCCAAACCTC TGAATCTGAG TCCAGACGCT CGCTGGGAAG TCGAGGCAGT 
ATGGGTCGCG AGCGCAGTGA AAAGCCTGTG AGTTTGGGGC CGTCACGACC GGGTCCTTTT 
GGCAGAGGTA GCAGCGCTAA GGAGCTAACA GATAACATGG TCCCAGAGGA AACACGGAGA 
GAAGGACAAG ACTCACACCA CACATCTCGC AAAGCCAGCA TCTCAGAGGA GAAATGTGAG 
ATGGAGAACA GCAGATCCGC AGAGTCAGTG AAATCAGAGA CGCTGCTGGT CAGCGGTGTT 
TCAGAGAAGC CTGTCCTGTC TGAAGAGGAG ATCGAGCGGA AGTCCAAAGC TATCATTGAT 
GAATTTCTGC ACATTAATGA CTACAAGGAG GCAGTGCAGT GTGTGCTGGA GATAGAGCAG 
CCCTCTATGC TGTGTGTGTT TGTGCGCATG GGTCTGGAGA GCACACTGGA GCGCAGTCAG 
AAAGCCAGAG AGCACATGGG ACTGCTCTAC TATCAGCTTA TACAAAAGGG CATCTTACCC 
CATTCTCAAC TCTACAAAGG GTTTTCAGAG ATGTTGGAGC AGGCTGATGA CATGGCCATA 
GACATTCCCT TCATTTGGCT TTATCTGGCT GAGCTGCTCA GTCCTCTGTT GAAAGAGGGA 
GGAATCAACA TGAGAGAGCT GTTCAGTGAG TTAAGTAAAC CGCTACTCCC TGTGGGAAGA 
GCTGCCATCT TATTGTCTGA AATACTGCAC CTACTATGCA AACAAATGAG CCATAGGAAA 
GTTGGAGACT TGTGGAGAGA GTCTGGGCTG AACTGGGCCG ACTTCCTGCC CGAGGGAGAA 
CATCTTCCTG CCTTCATCTC ACAACATAAG TTAGAATTCA CAGTGTCGGA CATCTCTCCT 
GTCTCTCCGG CCTCAGCCAA ACCCAGCCTC AGTCCTGAGG AGCTCTTTAA ACAACTGGAG 
CAGCTCCTGC TAGAGGACAT GTCCAGCGAT GAACAGATTT TTGACTGGAT CGAGGCTAAT 
TTGGACGAAT CTCAGATGAG TTCTTCTCCT TTTTTGAGAG CTTTAATGAC TGCTATATGT 
AAAGCAGCAG TTAAAGATGA AAGCACGTCC TGTCGAGTGG ACACTGCTAT CATCCAGAAA 
CGCCTGCCTA TATTACATAA GTACTTCGAC TCGGACACCG AGCGGCAGCT GCAAGCACTT 
TATGCTCTTC AGTCTCTGAT TGTCGCCCTG GATCAACCAC CCAACCTACT CCGGATGTTT 
TTCGATTGTC TCTACGATGA GGACGTCATT TCAGAGGACG CTTTCTATCA ATGGGAGACG 
AGTAAAGACC CGACAGAGCA GCTGGGAAAA GGCGTAGCGC TGAAGTCTGT GAACGCTTTC 
TTCACCTGGT TACGTGAAGC CGAGGAAGAA TCTGAGGATA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G3A ( XM_009306090.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304365.1 Danio rerio eukaryotic translation initiation factor 4 gamma, 3a (eif4g3a), transcript variant X2, mRNA.

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CloneID	ODa44792
Clone ID Related Accession (Same CDS sequence)	XM_009306090.3 , XM_009306090.2
Accession Version	XM_009306090.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	eukaryotic translation initiation factor 4 gamma 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306090.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTTGC CACCGAAAGT GGTTCCGAAA CCGGCCGCTG TCGCTGTTAG TGGACACGTA 
ACCGGACCGG CACCGCCAAC GCAGCTCCGC GCAGCGCTGA CCTCCGTGTC GTTGCCACCG 
GGAGCCCAAA ACGCTCCGCC GTCAGCCGTG CCGCCTACCC AGATTCCTAG AGCAGCGCTA 
TCACTGGATG AACGCATGTT TCCCGCTCAC TCTGGGGTGA CTGCGGTCTA CAGTGTATCC 
AGACACCCTG GTCCTCCATT TCCAGGTCAT GACCTCAGCA AAACCCACCC AAACCTGGCA 
GGGACTCCTC CTGGCCATGC CACGTCCCCG GCCCTCTCTC AGGTATCAGT TCCTGCTGGC 
CCTTCGTACC GCATCCTCAA GCCCTGGGAG ACAGGCGGAG CGCCTCCATA TAACCCAGCT 
CAGAATGCAG GCTCCGCCCC CCTGGTGTAC TCGCCACAGA CGCAGCCAAT GAACGTACAG 
CCACAGACTC GACCGTTTGT GACGGGACCT CGGCCAACAC ACCATCAGTT CATACACAGG 
AGTCAAATGC AGCCAGCGCG GCCTACCCTT CCGACTAACA ACCCCTCCAT TCGGCCGGGC 
TCACAGACCC CCACGGCGAC AGTTTATCCA CCAAACCAAC CCATCATGAT GACCATGACG 
CCCATGCCAT TTGCTACCCA GACACACCAA TATTACATTC CCCAGTATCG TCATAGTGCA 
CCTTACGTGG GACCCCCGCA GCAGTATGCC GTCCAGCCTC CAGGGTCTGG AACTTTCTAC 
CCTGGACCCA GCCCTGCGGA ATATCCCACC CCTTATGCTG CTGGTCCGCC ATATTACACC 
GGGCAGACGG TGTACCCACC CTCACCACCC ATCATAGTGC CTGCACCAAT GCCACCTCCA 
CCAACCAAGC GAGAAAAGAA ACCATCCTCC CAGATCCGGA TCCGAGACCC CAACCAGGGT 
GGAAAGGACA TCACAGAGGA AATCATGTTT GGGAGCCGCA ATCCTACACC TCCTGCCGGA 
CACCCAGCCT CCACCTTAAC CCCACCTGCA GGACGTCCCT CCTCGACTCC GACCCCACCT 
TCAGGACGCC TGTCCTCCAC ACCCACCCCT CCACAGCGGC CCAGCAACTG TCAAACCCCA 
GAACAAACGG CATATGTCAA CCAAAATCAG AGACTCTCAG AGAGCCCTGC GCCAATGGAT 
GGCAAGCCCA GCTTAGACGA CAGGCCAAAA ATGGAGTCTG GTCCCATTAA GTCTATATCT 
CCTGGACCAA GACCTTCAGA ATCCTGTTTG GAGAAAAGAG AAATCTCAAG TCTTCCATTA 
CTAGTCTCTT CTTCTCCAGA GGTGGATGTT TCTTCCCATC CTACAAGTGG ATGCATCAAA 
CCCACTGCCG CAGGAGAACC AGAATTTATC TCTCCTTCAG CCACTAAGGC TCAGACGTAT 
CAGGTCATCT CCGGTGAGGA ATCTGTTCCT GAAGCTTCTC CCAGATTGTC TGCTTCATTG 
TCCCTCAGAG TGGTAAATGG TGTCAATGAG CCTCAAACTC CCTCAAGCTA TGAAGAGCCT 
GAGGTCCAGG AAGCCCTAAA GATGTCGTCA TCCTGCGAGA TTCAGGGTAC ATCATTCATG 
GAGGAGTCTG GTCAGGAAGT TCCAGTGGCC TTGGAGGAGC TCCAGGCAGA GCACCTCCCT 
TCTCTAGCTG CACATGTGCC CTTGATCCCA GGCGTACAGG CCTCTTCAAT CACCTCCAGC 
ACCACTTCTG TTCTTGCGCC ACCACCTGGG TTGGCACCAT TAGTGCAGTC GGTTGTCCTT 
GAGGTGTCAG AACAGAACAA GACCTCAGAC GATGTCCAGC TGAAAGCTCA AGATGCTTCA 
GAATCACAAA CTCAAAGCAA CTCTAGAAAG TTTACACCAG CCCAAGCTGT CCCTGTTGTA 
CCAAAGCCTT GGAAGAAACC AAACGACGAA GATCTTATGA TGGAAACGCT GAAGAAAGAC 
AATGAGGAGG AGACCTCAAA GGACTGCTCT GCTGGAGATG AGATGGTCTT GCAGTCAAGC 
TTAGGGACCT ACAGCAGCCC CTTTACAGTG CCTGAGCACC AGGAGAAGCA CTCTGAAGAA 
AACGGTGAGA CCGAGACAGA GCCTTTCAGG AACGGGGTTG AGACGGAGAG CACGGACAGT 
GGTGTGACGC TCGGGGACAG TAGGGGCTCC ATGTGTTCCC CAACTCCACT CATTCAGGAA 
GGTCTTGTGC AATCTAATGG AAAGAGACAG TATGACCGGG ACTTCCTGTT GGGCTTTCAG 
TTTATGCCGG CCTGTGTGCA GAAGCCCGAG GGACTGCCTC CAATCAGTGA TGTGGTGCTA 
GACAAGATGA ACCAAAACAA GCTGCCTCTG AGGTCTGTGG ACCCCAGGTT GATTTCCAGA 
GGGACGCCTC AGGATTTCAC TCCTGCTTTT GCTGATTTCA GCAGGCAGAT GGCTGGAGGA 
CGCGGAGCCC CGCTTTTAAA TGTGCGACGA CTCCCACCAC GCAAGATCAT CGTAAACCTG 
TCTGTCAGTG ATGATGTTCA GCTCAAGAAG GCAGAAAATG CTTGGAAACC TGGGATGAAG 
AGAGAGGGTG TCGCAGAGGA CCCAGAAACC ATCAGAACCC AGGAGTTGTT CCGGAAAGTT 
CGTAGCATTT TAAACAAGCT CACGCCGCAG ATGTTTCATC AGCTGATGAA GCAAGTGACG 
GATCTTACTA TCAACACAGA GGAGAGGCTT AAAGGAGTCA TCGATCTTGT GTTTGAGAAG 
GCCATCGATG AGCCCAGCTT CTCTGTGGCC TACGCCAACA TGTGTCGCTG CCTGGCCACG 
CTCAAAGTGC CCACAGCAGA CAAACCTAAT ACAACGGTGA ACTTCCGGAA GCTCCTGTTG 
AATCGCTGTC AAAAGGAATT CGAGAGGGAC AAAGTAGATG ACGTTGTCCT GGAGAGAAAA 
CAAAAGGAGA TTGACTCTGC CACATCACCC ACAGAAAAGG AAAGACTGCA GGAGGAGCTG 
GAGGAGGCGA AGGACAAGGC CAGACGTCGC TCCACTGGAA ATATCAAATT CATCGGTGAG 
CTGTTCAAGC TCAAGATGTT GACAGAGCCC ATCATGCATG ACTGCGTGGT GAAACTGCTC 
AAAAACCACG ATGATGAGAG CTTAGAGTGC CTCTGCAGAC TCCTCACCAC CATCGGCAAA 
GACCTGGACT TCGAGAAGGC CAAGCCCCGA ATGGACCAGT ACTTCAACCA GATGGAGAAA 
ATTGTCAAAG AGCGCAAGAC CTCGTCTCGG ATACGGTTCA TGCTGCAGGA TGTGATAGAT 
CTGAGACTGC ATAACTGGGT TTCTCGGAGA GCTGACCAAG GCCCTAAAAC CATCGAGCAG 
ATCCATAAAG AGGCCAAGCT GGAGGAGCAG GAGGAGCAGA GGAAAGTCCA CCAGCAGCTT 
TTGTCTAAAG ACATGAAGAG GAGGCCAGAT CAGAGAGAAC AGCGTTCAAT GAGAGAGGAG 
ACTTGGAACA CTGTGCCCAC TACCAAAAAC AGCAGGACTA TCGACCCTAC AAAGATTCCA 
AAGATCTCCA AGCCGCAAAT CGATGAAAAG ATTCAGCTGG GTCCGCGAGC TCAGATCAAC 
TGGGTGAAAG GCAGCAGCGG CGGAGCAAAA GCCAGCGACT CTGAGTTGAC CCGCTCTGCC 
GGAAACACTC TGAACCGCTA TTCAGCACTG CAGTCATCCA CGTCTCAATC CAGTTCAACT 
CCGGCCCAAA CCTCTGAATC TGAGTCCAGA CGCTCGCTGG GAAGTCGAGG CAGTATGGGT 
CGCGAGCGCA GTGAAAAGCC TGTGAGTTTG GGGCCGTCAC GACCGGGTCC TTTTGGCAGA 
GGTAGCAGCG CTAAGGAGCT AACAGATAAC ATGGTCCCAG AGGAAACACG GAGAGAAGGA 
CAAGACTCAC ACCACACATC TCGCAAAGCC AGCATCTCAG AGGAGAAATG TGAGATGGAG 
AACAGCAGAT CCGCAGAGTC AGTGAAATCA GAGACGCTGC TGGTCAGCGG TGTTTCAGAG 
AAGCCTGTCC TGTCTGAAGA GGAGATCGAG CGGAAGTCCA AAGCTATCAT TGATGAATTT 
CTGCACATTA ATGACTACAA GGAGGCAGTG CAGTGTGTGC TGGAGATAGA GCAGCCCTCT 
ATGCTGTGTG TGTTTGTGCG CATGGGTCTG GAGAGCACAC TGGAGCGCAG TCAGAAAGCC 
AGAGAGCACA TGGGACTGCT CTACTATCAG CTTATACAAA AGGGCATCTT ACCCCATTCT 
CAACTCTACA AAGGGTTTTC AGAGATGTTG GAGCAGGCTG ATGACATGGC CATAGACATT 
CCCTTCATTT GGCTTTATCT GGCTGAGCTG CTCAGTCCTC TGTTGAAAGA GGGAGGAATC 
AACATGAGAG AGCTGTTCAG TGAGTTAAGT AAACCGCTAC TCCCTGTGGG AAGAGCTGCC 
ATCTTATTGT CTGAAATACT GCACCTACTA TGCAAACAAA TGAGCCATAG GAAAGTTGGA 
GACTTGTGGA GAGAGTCTGG GCTGAACTGG GCCGACTTCC TGCCCGAGGG AGAACATCTT 
CCTGCCTTCA TCTCACAACA TAAGTTAGAA TTCACAGTGT CGGACATCTC TCCTGTCTCT 
CCGGCCTCAG CCAAACCCAG CCTCAGTCCT GAGGAGCTCT TTAAACAACT GGAGCAGCTC 
CTGCTAGAGG ACATGTCCAG CGATGAACAG ATTTTTGACT GGATCGAGGC TAATTTGGAC 
GAATCTCAGA TGAGTTCTTC TCCTTTTTTG AGAGCTTTAA TGACTGCTAT ATGTAAAGCA 
GCAGTTAAAG ATGAAAGCAC GTCCTGTCGA GTGGACACTG CTATCATCCA GAAACGCCTG 
CCTATATTAC ATAAGTACTT CGACTCGGAC ACCGAGCGGC AGCTGCAAGC ACTTTATGCT 
CTTCAGTCTC TGATTGTCGC CCTGGATCAA CCACCCAACC TACTCCGGAT GTTTTTCGAT 
TGTCTCTACG ATGAGGACGT CATTTCAGAG GACGCTTTCT ATCAATGGGA GACGAGTAAA 
GACCCGACAG AGCAGCTGGG AAAAGGCGTA GCGCTGAAGT CTGTGAACGC TTTCTTCACC 
TGGTTACGTG AAGCCGAGGA AGAATCTGAG GATAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304365.1
CDS	460..5598
Translation	MSLPPKVVPKPAAVAVSGHVTGPAPPTQLRAALTSVSLPPGAQNAPPSAVPPTQIPRAALSLDERMFPAHSGVTAVYSVSRHPGPPFPGHDLSKTHPNLAGTPPGHATSPALSQVSVPAGPSYRILKPWETGGAPPYNPAQNAGSAPLVYSPQTQPMNVQPQTRPFVTGPRPTHHQFIHRSQMQPARPTLPTNNPSIRPGSQTPTATVYPPNQPIMMTMTPMPFATQTHQYYIPQYRHSAPYVGPPQQYAVQPPGSGTFYPGPSPAEYPTPYAAGPPYYTGQTVYPPSPPIIVPAPMPPPPTKREKKPSSQIRIRDPNQGGKDITEEIMFGSRNPTPPAGHPASTLTPPAGRPSSTPTPPSGRLSSTPTPPQRPSNCQTPEQTAYVNQNQRLSESPAPMDGKPSLDDRPKMESGPIKSISPGPRPSESCLEKREISSLPLLVSSSPEVDVSSHPTSGCIKPTAAGEPEFISPSATKAQTYQVISGEESVPEASPRLSASLSLRVVNGVNEPQTPSSYEEPEVQEALKMSSSCEIQGTSFMEESGQEVPVALEELQAEHLPSLAAHVPLIPGVQASSITSSTTSVLAPPPGLAPLVQSVVLEVSEQNKTSDDVQLKAQDASESQTQSNSRKFTPAQAVPVVPKPWKKPNDEDLMMETLKKDNEEETSKDCSAGDEMVLQSSLGTYSSPFTVPEHQEKHSEENGETETEPFRNGVETESTDSGVTLGDSRGSMCSPTPLIQEGLVQSNGKRQYDRDFLLGFQFMPACVQKPEGLPPISDVVLDKMNQNKLPLRSVDPRLISRGTPQDFTPAFADFSRQMAGGRGAPLLNVRRLPPRKIIVNLSVSDDVQLKKAENAWKPGMKREGVAEDPETIRTQELFRKVRSILNKLTPQMFHQLMKQVTDLTINTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLATLKVPTADKPNTTVNFRKLLLNRCQKEFERDKVDDVVLERKQKEIDSATSPTEKERLQEELEEAKDKARRRSTGNIKFIGELFKLKMLTEPIMHDCVVKLLKNHDDESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLHNWVSRRADQGPKTIEQIHKEAKLEEQEEQRKVHQQLLSKDMKRRPDQREQRSMREETWNTVPTTKNSRTIDPTKIPKISKPQIDEKIQLGPRAQINWVKGSSGGAKASDSELTRSAGNTLNRYSALQSSTSQSSSTPAQTSESESRRSLGSRGSMGRERSEKPVSLGPSRPGPFGRGSSAKELTDNMVPEETRREGQDSHHTSRKASISEEKCEMENSRSAESVKSETLLVSGVSEKPVLSEEEIERKSKAIIDEFLHINDYKEAVQCVLEIEQPSMLCVFVRMGLESTLERSQKAREHMGLLYYQLIQKGILPHSQLYKGFSEMLEQADDMAIDIPFIWLYLAELLSPLLKEGGINMRELFSELSKPLLPVGRAAILLSEILHLLCKQMSHRKVGDLWRESGLNWADFLPEGEHLPAFISQHKLEFTVSDISPVSPASAKPSLSPEELFKQLEQLLLEDMSSDEQIFDWIEANLDESQMSSSPFLRALMTAICKAAVKDESTSCRVDTAIIQKRLPILHKYFDSDTERQLQALYALQSLIVALDQPPNLLRMFFDCLYDEDVISEDAFYQWETSKDPTEQLGKGVALKSVNAFFTWLREAEEESEDN

Target ORF information:

RefSeq Version	XM_009306090.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio eukaryotic translation initiation factor 4 gamma, 3a (eif4g3a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306090.3

ORF Insert Sequence:

ATGAGTTTGC CACCGAAAGT GGTTCCGAAA CCGGCCGCTG TCGCTGTTAG TGGACACGTA 
ACCGGACCGG CACCGCCAAC GCAGCTCCGC GCAGCGCTGA CCTCCGTGTC GTTGCCACCG 
GGAGCCCAAA ACGCTCCGCC GTCAGCCGTG CCGCCTACCC AGATTCCTAG AGCAGCGCTA 
TCACTGGATG AACGCATGTT TCCCGCTCAC TCTGGGGTGA CTGCGGTCTA CAGTGTATCC 
AGACACCCTG GTCCTCCATT TCCAGGTCAT GACCTCAGCA AAACCCACCC AAACCTGGCA 
GGGACTCCTC CTGGCCATGC CACGTCCCCG GCCCTCTCTC AGGTATCAGT TCCTGCTGGC 
CCTTCGTACC GCATCCTCAA GCCCTGGGAG ACAGGCGGAG CGCCTCCATA TAACCCAGCT 
CAGAATGCAG GCTCCGCCCC CCTGGTGTAC TCGCCACAGA CGCAGCCAAT GAACGTACAG 
CCACAGACTC GACCGTTTGT GACGGGACCT CGGCCAACAC ACCATCAGTT CATACACAGG 
AGTCAAATGC AGCCAGCGCG GCCTACCCTT CCGACTAACA ACCCCTCCAT TCGGCCGGGC 
TCACAGACCC CCACGGCGAC AGTTTATCCA CCAAACCAAC CCATCATGAT GACCATGACG 
CCCATGCCAT TTGCTACCCA GACACACCAA TATTACATTC CCCAGTATCG TCATAGTGCA 
CCTTACGTGG GACCCCCGCA GCAGTATGCC GTCCAGCCTC CAGGGTCTGG AACTTTCTAC 
CCTGGACCCA GCCCTGCGGA ATATCCCACC CCTTATGCTG CTGGTCCGCC ATATTACACC 
GGGCAGACGG TGTACCCACC CTCACCACCC ATCATAGTGC CTGCACCAAT GCCACCTCCA 
CCAACCAAGC GAGAAAAGAA ACCATCCTCC CAGATCCGGA TCCGAGACCC CAACCAGGGT 
GGAAAGGACA TCACAGAGGA AATCATGTTT GGGAGCCGCA ATCCTACACC TCCTGCCGGA 
CACCCAGCCT CCACCTTAAC CCCACCTGCA GGACGTCCCT CCTCGACTCC GACCCCACCT 
TCAGGACGCC TGTCCTCCAC ACCCACCCCT CCACAGCGGC CCAGCAACTG TCAAACCCCA 
GAACAAACGG CATATGTCAA CCAAAATCAG AGACTCTCAG AGAGCCCTGC GCCAATGGAT 
GGCAAGCCCA GCTTAGACGA CAGGCCAAAA ATGGAGTCTG GTCCCATTAA GTCTATATCT 
CCTGGACCAA GACCTTCAGA ATCCTGTTTG GAGAAAAGAG AAATCTCAAG TCTTCCATTA 
CTAGTCTCTT CTTCTCCAGA GGTGGATGTT TCTTCCCATC CTACAAGTGG ATGCATCAAA 
CCCACTGCCG CAGGAGAACC AGAATTTATC TCTCCTTCAG CCACTAAGGC TCAGACGTAT 
CAGGTCATCT CCGGTGAGGA ATCTGTTCCT GAAGCTTCTC CCAGATTGTC TGCTTCATTG 
TCCCTCAGAG TGGTAAATGG TGTCAATGAG CCTCAAACTC CCTCAAGCTA TGAAGAGCCT 
GAGGTCCAGG AAGCCCTAAA GATGTCGTCA TCCTGCGAGA TTCAGGGTAC ATCATTCATG 
GAGGAGTCTG GTCAGGAAGT TCCAGTGGCC TTGGAGGAGC TCCAGGCAGA GCACCTCCCT 
TCTCTAGCTG CACATGTGCC CTTGATCCCA GGCGTACAGG CCTCTTCAAT CACCTCCAGC 
ACCACTTCTG TTCTTGCGCC ACCACCTGGG TTGGCACCAT TAGTGCAGTC GGTTGTCCTT 
GAGGTGTCAG AACAGAACAA GACCTCAGAC GATGTCCAGC TGAAAGCTCA AGATGCTTCA 
GAATCACAAA CTCAAAGCAA CTCTAGAAAG TTTACACCAG CCCAAGCTGT CCCTGTTGTA 
CCAAAGCCTT GGAAGAAACC AAACGACGAA GATCTTATGA TGGAAACGCT GAAGAAAGAC 
AATGAGGAGG AGACCTCAAA GGACTGCTCT GCTGGAGATG AGATGGTCTT GCAGTCAAGC 
TTAGGGACCT ACAGCAGCCC CTTTACAGTG CCTGAGCACC AGGAGAAGCA CTCTGAAGAA 
AACGGTGAGA CCGAGACAGA GCCTTTCAGG AACGGGGTTG AGACGGAGAG CACGGACAGT 
GGTGTGACGC TCGGGGACAG TAGGGGCTCC ATGTGTTCCC CAACTCCACT CATTCAGGAA 
GGTCTTGTGC AATCTAATGG AAAGAGACAG TATGACCGGG ACTTCCTGTT GGGCTTTCAG 
TTTATGCCGG CCTGTGTGCA GAAGCCCGAG GGACTGCCTC CAATCAGTGA TGTGGTGCTA 
GACAAGATGA ACCAAAACAA GCTGCCTCTG AGGTCTGTGG ACCCCAGGTT GATTTCCAGA 
GGGACGCCTC AGGATTTCAC TCCTGCTTTT GCTGATTTCA GCAGGCAGAT GGCTGGAGGA 
CGCGGAGCCC CGCTTTTAAA TGTGCGACGA CTCCCACCAC GCAAGATCAT CGTAAACCTG 
TCTGTCAGTG ATGATGTTCA GCTCAAGAAG GCAGAAAATG CTTGGAAACC TGGGATGAAG 
AGAGAGGGTG TCGCAGAGGA CCCAGAAACC ATCAGAACCC AGGAGTTGTT CCGGAAAGTT 
CGTAGCATTT TAAACAAGCT CACGCCGCAG ATGTTTCATC AGCTGATGAA GCAAGTGACG 
GATCTTACTA TCAACACAGA GGAGAGGCTT AAAGGAGTCA TCGATCTTGT GTTTGAGAAG 
GCCATCGATG AGCCCAGCTT CTCTGTGGCC TACGCCAACA TGTGTCGCTG CCTGGCCACG 
CTCAAAGTGC CCACAGCAGA CAAACCTAAT ACAACGGTGA ACTTCCGGAA GCTCCTGTTG 
AATCGCTGTC AAAAGGAATT CGAGAGGGAC AAAGTAGATG ACGTTGTCCT GGAGAGAAAA 
CAAAAGGAGA TTGACTCTGC CACATCACCC ACAGAAAAGG AAAGACTGCA GGAGGAGCTG 
GAGGAGGCGA AGGACAAGGC CAGACGTCGC TCCACTGGAA ATATCAAATT CATCGGTGAG 
CTGTTCAAGC TCAAGATGTT GACAGAGCCC ATCATGCATG ACTGCGTGGT GAAACTGCTC 
AAAAACCACG ATGATGAGAG CTTAGAGTGC CTCTGCAGAC TCCTCACCAC CATCGGCAAA 
GACCTGGACT TCGAGAAGGC CAAGCCCCGA ATGGACCAGT ACTTCAACCA GATGGAGAAA 
ATTGTCAAAG AGCGCAAGAC CTCGTCTCGG ATACGGTTCA TGCTGCAGGA TGTGATAGAT 
CTGAGACTGC ATAACTGGGT TTCTCGGAGA GCTGACCAAG GCCCTAAAAC CATCGAGCAG 
ATCCATAAAG AGGCCAAGCT GGAGGAGCAG GAGGAGCAGA GGAAAGTCCA CCAGCAGCTT 
TTGTCTAAAG ACATGAAGAG GAGGCCAGAT CAGAGAGAAC AGCGTTCAAT GAGAGAGGAG 
ACTTGGAACA CTGTGCCCAC TACCAAAAAC AGCAGGACTA TCGACCCTAC AAAGATTCCA 
AAGATCTCCA AGCCGCAAAT CGATGAAAAG ATTCAGCTGG GTCCGCGAGC TCAGATCAAC 
TGGGTGAAAG GCAGCAGCGG CGGAGCAAAA GCCAGCGACT CTGAGTTGAC CCGCTCTGCC 
GGAAACACTC TGAACCGCTA TTCAGCACTG CAGTCATCCA CGTCTCAATC CAGTTCAACT 
CCGGCCCAAA CCTCTGAATC TGAGTCCAGA CGCTCGCTGG GAAGTCGAGG CAGTATGGGT 
CGCGAGCGCA GTGAAAAGCC TGTGAGTTTG GGGCCGTCAC GACCGGGTCC TTTTGGCAGA 
GGTAGCAGCG CTAAGGAGCT AACAGATAAC ATGGTCCCAG AGGAAACACG GAGAGAAGGA 
CAAGACTCAC ACCACACATC TCGCAAAGCC AGCATCTCAG AGGAGAAATG TGAGATGGAG 
AACAGCAGAT CCGCAGAGTC AGTGAAATCA GAGACGCTGC TGGTCAGCGG TGTTTCAGAG 
AAGCCTGTCC TGTCTGAAGA GGAGATCGAG CGGAAGTCCA AAGCTATCAT TGATGAATTT 
CTGCACATTA ATGACTACAA GGAGGCAGTG CAGTGTGTGC TGGAGATAGA GCAGCCCTCT 
ATGCTGTGTG TGTTTGTGCG CATGGGTCTG GAGAGCACAC TGGAGCGCAG TCAGAAAGCC 
AGAGAGCACA TGGGACTGCT CTACTATCAG CTTATACAAA AGGGCATCTT ACCCCATTCT 
CAACTCTACA AAGGGTTTTC AGAGATGTTG GAGCAGGCTG ATGACATGGC CATAGACATT 
CCCTTCATTT GGCTTTATCT GGCTGAGCTG CTCAGTCCTC TGTTGAAAGA GGGAGGAATC 
AACATGAGAG AGCTGTTCAG TGAGTTAAGT AAACCGCTAC TCCCTGTGGG AAGAGCTGCC 
ATCTTATTGT CTGAAATACT GCACCTACTA TGCAAACAAA TGAGCCATAG GAAAGTTGGA 
GACTTGTGGA GAGAGTCTGG GCTGAACTGG GCCGACTTCC TGCCCGAGGG AGAACATCTT 
CCTGCCTTCA TCTCACAACA TAAGTTAGAA TTCACAGTGT CGGACATCTC TCCTGTCTCT 
CCGGCCTCAG CCAAACCCAG CCTCAGTCCT GAGGAGCTCT TTAAACAACT GGAGCAGCTC 
CTGCTAGAGG ACATGTCCAG CGATGAACAG ATTTTTGACT GGATCGAGGC TAATTTGGAC 
GAATCTCAGA TGAGTTCTTC TCCTTTTTTG AGAGCTTTAA TGACTGCTAT ATGTAAAGCA 
GCAGTTAAAG ATGAAAGCAC GTCCTGTCGA GTGGACACTG CTATCATCCA GAAACGCCTG 
CCTATATTAC ATAAGTACTT CGACTCGGAC ACCGAGCGGC AGCTGCAAGC ACTTTATGCT 
CTTCAGTCTC TGATTGTCGC CCTGGATCAA CCACCCAACC TACTCCGGAT GTTTTTCGAT 
TGTCTCTACG ATGAGGACGT CATTTCAGAG GACGCTTTCT ATCAATGGGA GACGAGTAAA 
GACCCGACAG AGCAGCTGGG AAAAGGCGTA GCGCTGAAGT CTGTGAACGC TTTCTTCACC 
TGGTTACGTG AAGCCGAGGA AGAATCTGAG GATAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF4G3A ( XM_009306090.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009304365.1 Danio rerio eukaryotic translation initiation factor 4 gamma, 3a (eif4g3a), transcript variant X2, mRNA.

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CloneID	ODa44792
Clone ID Related Accession (Same CDS sequence)	XM_009306090.3 , XM_009306090.2
Accession Version	XM_009306090.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5139bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	eukaryotic translation initiation factor 4 gamma 3 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306090.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTTGC CACCGAAAGT GGTTCCGAAA CCGGCCGCTG TCGCTGTTAG TGGACACGTA 
ACCGGACCGG CACCGCCAAC GCAGCTCCGC GCAGCGCTGA CCTCCGTGTC GTTGCCACCG 
GGAGCCCAAA ACGCTCCGCC GTCAGCCGTG CCGCCTACCC AGATTCCTAG AGCAGCGCTA 
TCACTGGATG AACGCATGTT TCCCGCTCAC TCTGGGGTGA CTGCGGTCTA CAGTGTATCC 
AGACACCCTG GTCCTCCATT TCCAGGTCAT GACCTCAGCA AAACCCACCC AAACCTGGCA 
GGGACTCCTC CTGGCCATGC CACGTCCCCG GCCCTCTCTC AGGTATCAGT TCCTGCTGGC 
CCTTCGTACC GCATCCTCAA GCCCTGGGAG ACAGGCGGAG CGCCTCCATA TAACCCAGCT 
CAGAATGCAG GCTCCGCCCC CCTGGTGTAC TCGCCACAGA CGCAGCCAAT GAACGTACAG 
CCACAGACTC GACCGTTTGT GACGGGACCT CGGCCAACAC ACCATCAGTT CATACACAGG 
AGTCAAATGC AGCCAGCGCG GCCTACCCTT CCGACTAACA ACCCCTCCAT TCGGCCGGGC 
TCACAGACCC CCACGGCGAC AGTTTATCCA CCAAACCAAC CCATCATGAT GACCATGACG 
CCCATGCCAT TTGCTACCCA GACACACCAA TATTACATTC CCCAGTATCG TCATAGTGCA 
CCTTACGTGG GACCCCCGCA GCAGTATGCC GTCCAGCCTC CAGGGTCTGG AACTTTCTAC 
CCTGGACCCA GCCCTGCGGA ATATCCCACC CCTTATGCTG CTGGTCCGCC ATATTACACC 
GGGCAGACGG TGTACCCACC CTCACCACCC ATCATAGTGC CTGCACCAAT GCCACCTCCA 
CCAACCAAGC GAGAAAAGAA ACCATCCTCC CAGATCCGGA TCCGAGACCC CAACCAGGGT 
GGAAAGGACA TCACAGAGGA AATCATGTTT GGGAGCCGCA ATCCTACACC TCCTGCCGGA 
CACCCAGCCT CCACCTTAAC CCCACCTGCA GGACGTCCCT CCTCGACTCC GACCCCACCT 
TCAGGACGCC TGTCCTCCAC ACCCACCCCT CCACAGCGGC CCAGCAACTG TCAAACCCCA 
GAACAAACGG CATATGTCAA CCAAAATCAG AGACTCTCAG AGAGCCCTGC GCCAATGGAT 
GGCAAGCCCA GCTTAGACGA CAGGCCAAAA ATGGAGTCTG GTCCCATTAA GTCTATATCT 
CCTGGACCAA GACCTTCAGA ATCCTGTTTG GAGAAAAGAG AAATCTCAAG TCTTCCATTA 
CTAGTCTCTT CTTCTCCAGA GGTGGATGTT TCTTCCCATC CTACAAGTGG ATGCATCAAA 
CCCACTGCCG CAGGAGAACC AGAATTTATC TCTCCTTCAG CCACTAAGGC TCAGACGTAT 
CAGGTCATCT CCGGTGAGGA ATCTGTTCCT GAAGCTTCTC CCAGATTGTC TGCTTCATTG 
TCCCTCAGAG TGGTAAATGG TGTCAATGAG CCTCAAACTC CCTCAAGCTA TGAAGAGCCT 
GAGGTCCAGG AAGCCCTAAA GATGTCGTCA TCCTGCGAGA TTCAGGGTAC ATCATTCATG 
GAGGAGTCTG GTCAGGAAGT TCCAGTGGCC TTGGAGGAGC TCCAGGCAGA GCACCTCCCT 
TCTCTAGCTG CACATGTGCC CTTGATCCCA GGCGTACAGG CCTCTTCAAT CACCTCCAGC 
ACCACTTCTG TTCTTGCGCC ACCACCTGGG TTGGCACCAT TAGTGCAGTC GGTTGTCCTT 
GAGGTGTCAG AACAGAACAA GACCTCAGAC GATGTCCAGC TGAAAGCTCA AGATGCTTCA 
GAATCACAAA CTCAAAGCAA CTCTAGAAAG TTTACACCAG CCCAAGCTGT CCCTGTTGTA 
CCAAAGCCTT GGAAGAAACC AAACGACGAA GATCTTATGA TGGAAACGCT GAAGAAAGAC 
AATGAGGAGG AGACCTCAAA GGACTGCTCT GCTGGAGATG AGATGGTCTT GCAGTCAAGC 
TTAGGGACCT ACAGCAGCCC CTTTACAGTG CCTGAGCACC AGGAGAAGCA CTCTGAAGAA 
AACGGTGAGA CCGAGACAGA GCCTTTCAGG AACGGGGTTG AGACGGAGAG CACGGACAGT 
GGTGTGACGC TCGGGGACAG TAGGGGCTCC ATGTGTTCCC CAACTCCACT CATTCAGGAA 
GGTCTTGTGC AATCTAATGG AAAGAGACAG TATGACCGGG ACTTCCTGTT GGGCTTTCAG 
TTTATGCCGG CCTGTGTGCA GAAGCCCGAG GGACTGCCTC CAATCAGTGA TGTGGTGCTA 
GACAAGATGA ACCAAAACAA GCTGCCTCTG AGGTCTGTGG ACCCCAGGTT GATTTCCAGA 
GGGACGCCTC AGGATTTCAC TCCTGCTTTT GCTGATTTCA GCAGGCAGAT GGCTGGAGGA 
CGCGGAGCCC CGCTTTTAAA TGTGCGACGA CTCCCACCAC GCAAGATCAT CGTAAACCTG 
TCTGTCAGTG ATGATGTTCA GCTCAAGAAG GCAGAAAATG CTTGGAAACC TGGGATGAAG 
AGAGAGGGTG TCGCAGAGGA CCCAGAAACC ATCAGAACCC AGGAGTTGTT CCGGAAAGTT 
CGTAGCATTT TAAACAAGCT CACGCCGCAG ATGTTTCATC AGCTGATGAA GCAAGTGACG 
GATCTTACTA TCAACACAGA GGAGAGGCTT AAAGGAGTCA TCGATCTTGT GTTTGAGAAG 
GCCATCGATG AGCCCAGCTT CTCTGTGGCC TACGCCAACA TGTGTCGCTG CCTGGCCACG 
CTCAAAGTGC CCACAGCAGA CAAACCTAAT ACAACGGTGA ACTTCCGGAA GCTCCTGTTG 
AATCGCTGTC AAAAGGAATT CGAGAGGGAC AAAGTAGATG ACGTTGTCCT GGAGAGAAAA 
CAAAAGGAGA TTGACTCTGC CACATCACCC ACAGAAAAGG AAAGACTGCA GGAGGAGCTG 
GAGGAGGCGA AGGACAAGGC CAGACGTCGC TCCACTGGAA ATATCAAATT CATCGGTGAG 
CTGTTCAAGC TCAAGATGTT GACAGAGCCC ATCATGCATG ACTGCGTGGT GAAACTGCTC 
AAAAACCACG ATGATGAGAG CTTAGAGTGC CTCTGCAGAC TCCTCACCAC CATCGGCAAA 
GACCTGGACT TCGAGAAGGC CAAGCCCCGA ATGGACCAGT ACTTCAACCA GATGGAGAAA 
ATTGTCAAAG AGCGCAAGAC CTCGTCTCGG ATACGGTTCA TGCTGCAGGA TGTGATAGAT 
CTGAGACTGC ATAACTGGGT TTCTCGGAGA GCTGACCAAG GCCCTAAAAC CATCGAGCAG 
ATCCATAAAG AGGCCAAGCT GGAGGAGCAG GAGGAGCAGA GGAAAGTCCA CCAGCAGCTT 
TTGTCTAAAG ACATGAAGAG GAGGCCAGAT CAGAGAGAAC AGCGTTCAAT GAGAGAGGAG 
ACTTGGAACA CTGTGCCCAC TACCAAAAAC AGCAGGACTA TCGACCCTAC AAAGATTCCA 
AAGATCTCCA AGCCGCAAAT CGATGAAAAG ATTCAGCTGG GTCCGCGAGC TCAGATCAAC 
TGGGTGAAAG GCAGCAGCGG CGGAGCAAAA GCCAGCGACT CTGAGTTGAC CCGCTCTGCC 
GGAAACACTC TGAACCGCTA TTCAGCACTG CAGTCATCCA CGTCTCAATC CAGTTCAACT 
CCGGCCCAAA CCTCTGAATC TGAGTCCAGA CGCTCGCTGG GAAGTCGAGG CAGTATGGGT 
CGCGAGCGCA GTGAAAAGCC TGTGAGTTTG GGGCCGTCAC GACCGGGTCC TTTTGGCAGA 
GGTAGCAGCG CTAAGGAGCT AACAGATAAC ATGGTCCCAG AGGAAACACG GAGAGAAGGA 
CAAGACTCAC ACCACACATC TCGCAAAGCC AGCATCTCAG AGGAGAAATG TGAGATGGAG 
AACAGCAGAT CCGCAGAGTC AGTGAAATCA GAGACGCTGC TGGTCAGCGG TGTTTCAGAG 
AAGCCTGTCC TGTCTGAAGA GGAGATCGAG CGGAAGTCCA AAGCTATCAT TGATGAATTT 
CTGCACATTA ATGACTACAA GGAGGCAGTG CAGTGTGTGC TGGAGATAGA GCAGCCCTCT 
ATGCTGTGTG TGTTTGTGCG CATGGGTCTG GAGAGCACAC TGGAGCGCAG TCAGAAAGCC 
AGAGAGCACA TGGGACTGCT CTACTATCAG CTTATACAAA AGGGCATCTT ACCCCATTCT 
CAACTCTACA AAGGGTTTTC AGAGATGTTG GAGCAGGCTG ATGACATGGC CATAGACATT 
CCCTTCATTT GGCTTTATCT GGCTGAGCTG CTCAGTCCTC TGTTGAAAGA GGGAGGAATC 
AACATGAGAG AGCTGTTCAG TGAGTTAAGT AAACCGCTAC TCCCTGTGGG AAGAGCTGCC 
ATCTTATTGT CTGAAATACT GCACCTACTA TGCAAACAAA TGAGCCATAG GAAAGTTGGA 
GACTTGTGGA GAGAGTCTGG GCTGAACTGG GCCGACTTCC TGCCCGAGGG AGAACATCTT 
CCTGCCTTCA TCTCACAACA TAAGTTAGAA TTCACAGTGT CGGACATCTC TCCTGTCTCT 
CCGGCCTCAG CCAAACCCAG CCTCAGTCCT GAGGAGCTCT TTAAACAACT GGAGCAGCTC 
CTGCTAGAGG ACATGTCCAG CGATGAACAG ATTTTTGACT GGATCGAGGC TAATTTGGAC 
GAATCTCAGA TGAGTTCTTC TCCTTTTTTG AGAGCTTTAA TGACTGCTAT ATGTAAAGCA 
GCAGTTAAAG ATGAAAGCAC GTCCTGTCGA GTGGACACTG CTATCATCCA GAAACGCCTG 
CCTATATTAC ATAAGTACTT CGACTCGGAC ACCGAGCGGC AGCTGCAAGC ACTTTATGCT 
CTTCAGTCTC TGATTGTCGC CCTGGATCAA CCACCCAACC TACTCCGGAT GTTTTTCGAT 
TGTCTCTACG ATGAGGACGT CATTTCAGAG GACGCTTTCT ATCAATGGGA GACGAGTAAA 
GACCCGACAG AGCAGCTGGG AAAAGGCGTA GCGCTGAAGT CTGTGAACGC TTTCTTCACC 
TGGTTACGTG AAGCCGAGGA AGAATCTGAG GATAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304365.1
CDS	489..5627
Translation	MSLPPKVVPKPAAVAVSGHVTGPAPPTQLRAALTSVSLPPGAQNAPPSAVPPTQIPRAALSLDERMFPAHSGVTAVYSVSRHPGPPFPGHDLSKTHPNLAGTPPGHATSPALSQVSVPAGPSYRILKPWETGGAPPYNPAQNAGSAPLVYSPQTQPMNVQPQTRPFVTGPRPTHHQFIHRSQMQPARPTLPTNNPSIRPGSQTPTATVYPPNQPIMMTMTPMPFATQTHQYYIPQYRHSAPYVGPPQQYAVQPPGSGTFYPGPSPAEYPTPYAAGPPYYTGQTVYPPSPPIIVPAPMPPPPTKREKKPSSQIRIRDPNQGGKDITEEIMFGSRNPTPPAGHPASTLTPPAGRPSSTPTPPSGRLSSTPTPPQRPSNCQTPEQTAYVNQNQRLSESPAPMDGKPSLDDRPKMESGPIKSISPGPRPSESCLEKREISSLPLLVSSSPEVDVSSHPTSGCIKPTAAGEPEFISPSATKAQTYQVISGEESVPEASPRLSASLSLRVVNGVNEPQTPSSYEEPEVQEALKMSSSCEIQGTSFMEESGQEVPVALEELQAEHLPSLAAHVPLIPGVQASSITSSTTSVLAPPPGLAPLVQSVVLEVSEQNKTSDDVQLKAQDASESQTQSNSRKFTPAQAVPVVPKPWKKPNDEDLMMETLKKDNEEETSKDCSAGDEMVLQSSLGTYSSPFTVPEHQEKHSEENGETETEPFRNGVETESTDSGVTLGDSRGSMCSPTPLIQEGLVQSNGKRQYDRDFLLGFQFMPACVQKPEGLPPISDVVLDKMNQNKLPLRSVDPRLISRGTPQDFTPAFADFSRQMAGGRGAPLLNVRRLPPRKIIVNLSVSDDVQLKKAENAWKPGMKREGVAEDPETIRTQELFRKVRSILNKLTPQMFHQLMKQVTDLTINTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLATLKVPTADKPNTTVNFRKLLLNRCQKEFERDKVDDVVLERKQKEIDSATSPTEKERLQEELEEAKDKARRRSTGNIKFIGELFKLKMLTEPIMHDCVVKLLKNHDDESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQDVIDLRLHNWVSRRADQGPKTIEQIHKEAKLEEQEEQRKVHQQLLSKDMKRRPDQREQRSMREETWNTVPTTKNSRTIDPTKIPKISKPQIDEKIQLGPRAQINWVKGSSGGAKASDSELTRSAGNTLNRYSALQSSTSQSSSTPAQTSESESRRSLGSRGSMGRERSEKPVSLGPSRPGPFGRGSSAKELTDNMVPEETRREGQDSHHTSRKASISEEKCEMENSRSAESVKSETLLVSGVSEKPVLSEEEIERKSKAIIDEFLHINDYKEAVQCVLEIEQPSMLCVFVRMGLESTLERSQKAREHMGLLYYQLIQKGILPHSQLYKGFSEMLEQADDMAIDIPFIWLYLAELLSPLLKEGGINMRELFSELSKPLLPVGRAAILLSEILHLLCKQMSHRKVGDLWRESGLNWADFLPEGEHLPAFISQHKLEFTVSDISPVSPASAKPSLSPEELFKQLEQLLLEDMSSDEQIFDWIEANLDESQMSSSPFLRALMTAICKAAVKDESTSCRVDTAIIQKRLPILHKYFDSDTERQLQALYALQSLIVALDQPPNLLRMFFDCLYDEDVISEDAFYQWETSKDPTEQLGKGVALKSVNAFFTWLREAEEESEDN

Target ORF information:

RefSeq Version	XM_009306090.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio eukaryotic translation initiation factor 4 gamma, 3a (eif4g3a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306090.2

ORF Insert Sequence:

ATGAGTTTGC CACCGAAAGT GGTTCCGAAA CCGGCCGCTG TCGCTGTTAG TGGACACGTA 
ACCGGACCGG CACCGCCAAC GCAGCTCCGC GCAGCGCTGA CCTCCGTGTC GTTGCCACCG 
GGAGCCCAAA ACGCTCCGCC GTCAGCCGTG CCGCCTACCC AGATTCCTAG AGCAGCGCTA 
TCACTGGATG AACGCATGTT TCCCGCTCAC TCTGGGGTGA CTGCGGTCTA CAGTGTATCC 
AGACACCCTG GTCCTCCATT TCCAGGTCAT GACCTCAGCA AAACCCACCC AAACCTGGCA 
GGGACTCCTC CTGGCCATGC CACGTCCCCG GCCCTCTCTC AGGTATCAGT TCCTGCTGGC 
CCTTCGTACC GCATCCTCAA GCCCTGGGAG ACAGGCGGAG CGCCTCCATA TAACCCAGCT 
CAGAATGCAG GCTCCGCCCC CCTGGTGTAC TCGCCACAGA CGCAGCCAAT GAACGTACAG 
CCACAGACTC GACCGTTTGT GACGGGACCT CGGCCAACAC ACCATCAGTT CATACACAGG 
AGTCAAATGC AGCCAGCGCG GCCTACCCTT CCGACTAACA ACCCCTCCAT TCGGCCGGGC 
TCACAGACCC CCACGGCGAC AGTTTATCCA CCAAACCAAC CCATCATGAT GACCATGACG 
CCCATGCCAT TTGCTACCCA GACACACCAA TATTACATTC CCCAGTATCG TCATAGTGCA 
CCTTACGTGG GACCCCCGCA GCAGTATGCC GTCCAGCCTC CAGGGTCTGG AACTTTCTAC 
CCTGGACCCA GCCCTGCGGA ATATCCCACC CCTTATGCTG CTGGTCCGCC ATATTACACC 
GGGCAGACGG TGTACCCACC CTCACCACCC ATCATAGTGC CTGCACCAAT GCCACCTCCA 
CCAACCAAGC GAGAAAAGAA ACCATCCTCC CAGATCCGGA TCCGAGACCC CAACCAGGGT 
GGAAAGGACA TCACAGAGGA AATCATGTTT GGGAGCCGCA ATCCTACACC TCCTGCCGGA 
CACCCAGCCT CCACCTTAAC CCCACCTGCA GGACGTCCCT CCTCGACTCC GACCCCACCT 
TCAGGACGCC TGTCCTCCAC ACCCACCCCT CCACAGCGGC CCAGCAACTG TCAAACCCCA 
GAACAAACGG CATATGTCAA CCAAAATCAG AGACTCTCAG AGAGCCCTGC GCCAATGGAT 
GGCAAGCCCA GCTTAGACGA CAGGCCAAAA ATGGAGTCTG GTCCCATTAA GTCTATATCT 
CCTGGACCAA GACCTTCAGA ATCCTGTTTG GAGAAAAGAG AAATCTCAAG TCTTCCATTA 
CTAGTCTCTT CTTCTCCAGA GGTGGATGTT TCTTCCCATC CTACAAGTGG ATGCATCAAA 
CCCACTGCCG CAGGAGAACC AGAATTTATC TCTCCTTCAG CCACTAAGGC TCAGACGTAT 
CAGGTCATCT CCGGTGAGGA ATCTGTTCCT GAAGCTTCTC CCAGATTGTC TGCTTCATTG 
TCCCTCAGAG TGGTAAATGG TGTCAATGAG CCTCAAACTC CCTCAAGCTA TGAAGAGCCT 
GAGGTCCAGG AAGCCCTAAA GATGTCGTCA TCCTGCGAGA TTCAGGGTAC ATCATTCATG 
GAGGAGTCTG GTCAGGAAGT TCCAGTGGCC TTGGAGGAGC TCCAGGCAGA GCACCTCCCT 
TCTCTAGCTG CACATGTGCC CTTGATCCCA GGCGTACAGG CCTCTTCAAT CACCTCCAGC 
ACCACTTCTG TTCTTGCGCC ACCACCTGGG TTGGCACCAT TAGTGCAGTC GGTTGTCCTT 
GAGGTGTCAG AACAGAACAA GACCTCAGAC GATGTCCAGC TGAAAGCTCA AGATGCTTCA 
GAATCACAAA CTCAAAGCAA CTCTAGAAAG TTTACACCAG CCCAAGCTGT CCCTGTTGTA 
CCAAAGCCTT GGAAGAAACC AAACGACGAA GATCTTATGA TGGAAACGCT GAAGAAAGAC 
AATGAGGAGG AGACCTCAAA GGACTGCTCT GCTGGAGATG AGATGGTCTT GCAGTCAAGC 
TTAGGGACCT ACAGCAGCCC CTTTACAGTG CCTGAGCACC AGGAGAAGCA CTCTGAAGAA 
AACGGTGAGA CCGAGACAGA GCCTTTCAGG AACGGGGTTG AGACGGAGAG CACGGACAGT 
GGTGTGACGC TCGGGGACAG TAGGGGCTCC ATGTGTTCCC CAACTCCACT CATTCAGGAA 
GGTCTTGTGC AATCTAATGG AAAGAGACAG TATGACCGGG ACTTCCTGTT GGGCTTTCAG 
TTTATGCCGG CCTGTGTGCA GAAGCCCGAG GGACTGCCTC CAATCAGTGA TGTGGTGCTA 
GACAAGATGA ACCAAAACAA GCTGCCTCTG AGGTCTGTGG ACCCCAGGTT GATTTCCAGA 
GGGACGCCTC AGGATTTCAC TCCTGCTTTT GCTGATTTCA GCAGGCAGAT GGCTGGAGGA 
CGCGGAGCCC CGCTTTTAAA TGTGCGACGA CTCCCACCAC GCAAGATCAT CGTAAACCTG 
TCTGTCAGTG ATGATGTTCA GCTCAAGAAG GCAGAAAATG CTTGGAAACC TGGGATGAAG 
AGAGAGGGTG TCGCAGAGGA CCCAGAAACC ATCAGAACCC AGGAGTTGTT CCGGAAAGTT 
CGTAGCATTT TAAACAAGCT CACGCCGCAG ATGTTTCATC AGCTGATGAA GCAAGTGACG 
GATCTTACTA TCAACACAGA GGAGAGGCTT AAAGGAGTCA TCGATCTTGT GTTTGAGAAG 
GCCATCGATG AGCCCAGCTT CTCTGTGGCC TACGCCAACA TGTGTCGCTG CCTGGCCACG 
CTCAAAGTGC CCACAGCAGA CAAACCTAAT ACAACGGTGA ACTTCCGGAA GCTCCTGTTG 
AATCGCTGTC AAAAGGAATT CGAGAGGGAC AAAGTAGATG ACGTTGTCCT GGAGAGAAAA 
CAAAAGGAGA TTGACTCTGC CACATCACCC ACAGAAAAGG AAAGACTGCA GGAGGAGCTG 
GAGGAGGCGA AGGACAAGGC CAGACGTCGC TCCACTGGAA ATATCAAATT CATCGGTGAG 
CTGTTCAAGC TCAAGATGTT GACAGAGCCC ATCATGCATG ACTGCGTGGT GAAACTGCTC 
AAAAACCACG ATGATGAGAG CTTAGAGTGC CTCTGCAGAC TCCTCACCAC CATCGGCAAA 
GACCTGGACT TCGAGAAGGC CAAGCCCCGA ATGGACCAGT ACTTCAACCA GATGGAGAAA 
ATTGTCAAAG AGCGCAAGAC CTCGTCTCGG ATACGGTTCA TGCTGCAGGA TGTGATAGAT 
CTGAGACTGC ATAACTGGGT TTCTCGGAGA GCTGACCAAG GCCCTAAAAC CATCGAGCAG 
ATCCATAAAG AGGCCAAGCT GGAGGAGCAG GAGGAGCAGA GGAAAGTCCA CCAGCAGCTT 
TTGTCTAAAG ACATGAAGAG GAGGCCAGAT CAGAGAGAAC AGCGTTCAAT GAGAGAGGAG 
ACTTGGAACA CTGTGCCCAC TACCAAAAAC AGCAGGACTA TCGACCCTAC AAAGATTCCA 
AAGATCTCCA AGCCGCAAAT CGATGAAAAG ATTCAGCTGG GTCCGCGAGC TCAGATCAAC 
TGGGTGAAAG GCAGCAGCGG CGGAGCAAAA GCCAGCGACT CTGAGTTGAC CCGCTCTGCC 
GGAAACACTC TGAACCGCTA TTCAGCACTG CAGTCATCCA CGTCTCAATC CAGTTCAACT 
CCGGCCCAAA CCTCTGAATC TGAGTCCAGA CGCTCGCTGG GAAGTCGAGG CAGTATGGGT 
CGCGAGCGCA GTGAAAAGCC TGTGAGTTTG GGGCCGTCAC GACCGGGTCC TTTTGGCAGA 
GGTAGCAGCG CTAAGGAGCT AACAGATAAC ATGGTCCCAG AGGAAACACG GAGAGAAGGA 
CAAGACTCAC ACCACACATC TCGCAAAGCC AGCATCTCAG AGGAGAAATG TGAGATGGAG 
AACAGCAGAT CCGCAGAGTC AGTGAAATCA GAGACGCTGC TGGTCAGCGG TGTTTCAGAG 
AAGCCTGTCC TGTCTGAAGA GGAGATCGAG CGGAAGTCCA AAGCTATCAT TGATGAATTT 
CTGCACATTA ATGACTACAA GGAGGCAGTG CAGTGTGTGC TGGAGATAGA GCAGCCCTCT 
ATGCTGTGTG TGTTTGTGCG CATGGGTCTG GAGAGCACAC TGGAGCGCAG TCAGAAAGCC 
AGAGAGCACA TGGGACTGCT CTACTATCAG CTTATACAAA AGGGCATCTT ACCCCATTCT 
CAACTCTACA AAGGGTTTTC AGAGATGTTG GAGCAGGCTG ATGACATGGC CATAGACATT 
CCCTTCATTT GGCTTTATCT GGCTGAGCTG CTCAGTCCTC TGTTGAAAGA GGGAGGAATC 
AACATGAGAG AGCTGTTCAG TGAGTTAAGT AAACCGCTAC TCCCTGTGGG AAGAGCTGCC 
ATCTTATTGT CTGAAATACT GCACCTACTA TGCAAACAAA TGAGCCATAG GAAAGTTGGA 
GACTTGTGGA GAGAGTCTGG GCTGAACTGG GCCGACTTCC TGCCCGAGGG AGAACATCTT 
CCTGCCTTCA TCTCACAACA TAAGTTAGAA TTCACAGTGT CGGACATCTC TCCTGTCTCT 
CCGGCCTCAG CCAAACCCAG CCTCAGTCCT GAGGAGCTCT TTAAACAACT GGAGCAGCTC 
CTGCTAGAGG ACATGTCCAG CGATGAACAG ATTTTTGACT GGATCGAGGC TAATTTGGAC 
GAATCTCAGA TGAGTTCTTC TCCTTTTTTG AGAGCTTTAA TGACTGCTAT ATGTAAAGCA 
GCAGTTAAAG ATGAAAGCAC GTCCTGTCGA GTGGACACTG CTATCATCCA GAAACGCCTG 
CCTATATTAC ATAAGTACTT CGACTCGGAC ACCGAGCGGC AGCTGCAAGC ACTTTATGCT 
CTTCAGTCTC TGATTGTCGC CCTGGATCAA CCACCCAACC TACTCCGGAT GTTTTTCGAT 
TGTCTCTACG ATGAGGACGT CATTTCAGAG GACGCTTTCT ATCAATGGGA GACGAGTAAA 
GACCCGACAG AGCAGCTGGG AAAAGGCGTA GCGCTGAAGT CTGTGAACGC TTTCTTCACC 
TGGTTACGTG AAGCCGAGGA AGAATCTGAG GATAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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EIF4G3A cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

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