tjap1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tjap1
Entrez Gene ID	566060
Full Name	tight junction associated protein 1 (peripheral)
General protein information	Preferred Names tight junction associated protein 1 (peripheral) Names tight junction-associated protein 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_001921650.5	XP_001921685.4	tight junction-associated protein 1 isoform X2
XM_009307071.1	XP_009305346.1	tight junction-associated protein 1 isoform X1
XM_021480930.1	XP_021336605.1	tight junction-associated protein 1
NM_001365539.1	NP_001352468.1	tight junction-associated protein 1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	TJAP1	XP_419505.3
Homo sapiens (human)	TJAP1	NP_001139489.1
Rattus norvegicus (Norway rat)	Tjap1	NP_001101673.1
Canis lupus familiaris (dog)	TJAP1	XP_005627447.1
Bos taurus (cattle)	TJAP1	NP_001179348.1
Pan troglodytes (chimpanzee)	TJAP1	XP_001138031.1
	TJAP1	XP_002803803.1
Mus musculus (house mouse)	Tjap1	NP_001239402.1
Danio rerio (zebrafish)	LOC566060	XP_001921685.4

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following tjap1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tjap1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa18386	NM_001365539.1 Latest version!	Danio rerio tight junction associated protein 1 (peripheral) (tjap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa18386	XM_001921650.5 Latest version!	Danio rerio tight junction associated protein 1 (peripheral) (tjap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa44781	XM_009307071.1 Latest version!	Danio rerio tight junction associated protein 1 (peripheral) (tjap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa148685	XM_021480930.1 Latest version!	Danio rerio tight junction associated protein 1 (peripheral) (tjap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tjap1 ( NM_001365539.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001352468.1 Danio rerio tight junction associated protein 1 (peripheral) (tjap1), mRNA.

Price & Availability↑

CloneID	ODa18386
Clone ID Related Accession (Same CDS sequence)	XM_001921650.5 , NM_001365539.1
Accession Version	NM_001365539.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-21
Organism	Danio rerio(zebrafish)
Product	tight junction-associated protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX510320.6, CT029995.8 and CU570792.8. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01023897.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGACGAGTG CTGCACCTGC CCGGAAACCT TACCGAAAGG CACCTCCGCA GCACCGCGAG 
ACGCGTCACA CTGTGCCAGT ATTTCGAGAG GATGTTAGCG GGAGTGCCTT GCCATCCGCC 
AACCCTGCAA CGTCAGCCCA GCCTGACCCC TGCCAGGAGT CCTTATCAGA TGCCGACAGG 
ATCAAAATCC TGCAGCAGCA AAATGAAGAC CTCCGGCGCC GACTGTCCCA GACCACACAT 
AAGATGGAGG CCATGGAGAC GGAGTTCGAA ACCAGTCGAC ATTACATGCA GGCGGAGCTT 
GGTCGAACTA GAGACGACCT GGAGAAAATG AGAGACAAGT TTAGAAGACT TCAGAACAGC 
TACACGGCAT CTCAGAGAGC TAATCAAGAC CTGGAGGAGA AACTTCACGC CCTGCTCCGT 
AAAGTGGAAA GAGACAAGAA AACTATGGAT CAAGAGATAG TGGAGCTCAC CAATAAACTT 
GTGGACGCCA AAAATACCAT CGACAAGCTG GAGGAGCTGA ACGAACGCTA TCGACAGGAT 
TGCAATCTGG CCGTGCAGCT GTTGAAGTGC AATAAATCGC ATTTCAGGAA CCACAAGTTT 
GCTGATTTGC CATATGAGCT ACAGGACATG GTGAACAAGC ATATGAAGAG CAGCTTGCCC 
AACAAGACTC AGGGAGCTCA AGCCCAGGAT CCTGACACTC TAAGCCTGAC TCCTGCAGAT 
GTAGTGCCCA CTTCGGTCAT TGCTCGTGTT CTTGAGAAAC CCGAGCCCCT GGTGCTCAAC 
TCGGCTCAGT CTAGCAGCAG TGCAGGTCGG CCTGTGGCTG AGGATGTCTT TGTACACGTG 
GACATGACGG GACCACAAGA TGATGATGGA GGGCGGGAAA ATGGTGGTCC TGTTCCTGCT 
AGGCCTCCTG CGGTAGCTGA TACTGACCAG CAGCATGTCA ATGGCATGTG TCGAAGCCAG 
GGCAGCCTTG ATGGTCCTAC AGGAGATGAT GGTGCAGCTC CCTCTTTCGA GAAGCTGAAT 
CCTTACCCTA CTCCTTCACC ACCAAACCCG CTCTACCCAG GTCGCAAAGT GATTGAGTTT 
TCTTCTGATG ATAAAGTCAA GATCCCCAAG AACAGCCCAC TGCCCAATTG CACCTACGCT 
ACTCGTCAGG CTATTTCTTT GAGTCTTGTG CAGAATGAAG AAGAGGCAGC CGAGCGCCAG 
CGCACAGTGC CTAATAGCCC TGCCATATCT GATGGGGGTT GGCGATCTGG AACCTCCTCA 
TCTTCCGGTG GAGGGATTGC TTCTCACCAG AGGACACCTC CACAGATGGA TGCTACAGAC 
ACACTCTCCA GTCATTCAAG TCCCTTTAGC AGCCCCCCTC AGCCGCCCAG CGCCTTTGCC 
AGTTCTGGAA GCTCCGAGGA GGACCTTCTA GCCAGCTGGC AGCGCATGTT TGTTGAAAAA 
ATGGCACCTT CTTCAGATGG CTCCCTTGTC AACCGCACAT CCTTCAGCAG TGAGACGGTA 
AAAGATCTCC AAAGAAGTCA TAATGGAAAA TCAGGAGTTG GTGCTCTGAG AGGTGCTTAC 
TCTGATGGTG AGGAGGGATC CTCGGCTCAG AGCTGGACAG CAAGTAGAGA GTCAAGTTTG 
GACACAGACA CCAGCAGCAT GGCAGACCTT CGGGCAAAGA AGACACAATA TGGTGGCGAC 
TTTTCCCATG AAGAAGGTGA ACACCTTCTG ATGGCTCTGG ATCCACTTGA TAATGATGGT 
GCTAGCGGTG ACACTATGGT CACCATTGAG AGTAGTCCTG CCTCTGCGAA GCGTCAAGAG 
TTTGCAGAAG ATGTTGGATC GGCGGGAAGT TCAGCAGAAG AGCGAGATGT CCTTCCCCAG 
GACTTTCCTG TCATTGGCTC AAGGGTTCTT TCTTCCCTTG AGGATTATGC AGATAAGCCC 
CCTCGGACTG GCTCCTCGCG ACCTCTGAAG AGTCCCAAGA GGATGGGGGT TCATCACTTG 
CACCGCAAAG ACAGCCTTAC CAGAGCCCAG GAGCATGGCA ACTTGCTAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001352468.1
CDS	793..2826
Translation	MTSAAPARKPYRKAPPQHRETRHTVPVFREDVSGSALPSANPATSAQPDPCQESLSDADRIKILQQQNEDLRRRLSQTTHKMEAMETEFETSRHYMQAELGRTRDDLEKMRDKFRRLQNSYTASQRANQDLEEKLHALLRKVERDKKTMDQEIVELTNKLVDAKNTIDKLEELNERYRQDCNLAVQLLKCNKSHFRNHKFADLPYELQDMVNKHMKSSLPNKTQGAQAQDPDTLSLTPADVVPTSVIARVLEKPEPLVLNSAQSSSSAGRPVAEDVFVHVDMTGPQDDDGGRENGGPVPARPPAVADTDQQHVNGMCRSQGSLDGPTGDDGAAPSFEKLNPYPTPSPPNPLYPGRKVIEFSSDDKVKIPKNSPLPNCTYATRQAISLSLVQNEEEAAERQRTVPNSPAISDGGWRSGTSSSSGGGIASHQRTPPQMDATDTLSSHSSPFSSPPQPPSAFASSGSSEEDLLASWQRMFVEKMAPSSDGSLVNRTSFSSETVKDLQRSHNGKSGVGALRGAYSDGEEGSSAQSWTASRESSLDTDTSSMADLRAKKTQYGGDFSHEEGEHLLMALDPLDNDGASGDTMVTIESSPASAKRQEFAEDVGSAGSSAEERDVLPQDFPVIGSRVLSSLEDYADKPPRTGSSRPLKSPKRMGVHHLHRKDSLTRAQEHGNLLD

Target ORF information:

RefSeq Version	NM_001365539.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction associated protein 1 (peripheral) (tjap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001365539.1

ORF Insert Sequence:

ATGACGAGTG CTGCACCTGC CCGGAAACCT TACCGAAAGG CACCTCCGCA GCACCGCGAG 
ACGCGTCACA CTGTGCCAGT ATTTCGAGAG GATGTTAGCG GGAGTGCCTT GCCATCCGCC 
AACCCTGCAA CGTCAGCCCA GCCTGACCCC TGCCAGGAGT CCTTATCAGA TGCCGACAGG 
ATCAAAATCC TGCAGCAGCA AAATGAAGAC CTCCGGCGCC GACTGTCCCA GACCACACAT 
AAGATGGAGG CCATGGAGAC GGAGTTCGAA ACCAGTCGAC ATTACATGCA GGCGGAGCTT 
GGTCGAACTA GAGACGACCT GGAGAAAATG AGAGACAAGT TTAGAAGACT TCAGAACAGC 
TACACGGCAT CTCAGAGAGC TAATCAAGAC CTGGAGGAGA AACTTCACGC CCTGCTCCGT 
AAAGTGGAAA GAGACAAGAA AACTATGGAT CAAGAGATAG TGGAGCTCAC CAATAAACTT 
GTGGACGCCA AAAATACCAT CGACAAGCTG GAGGAGCTGA ACGAACGCTA TCGACAGGAT 
TGCAATCTGG CCGTGCAGCT GTTGAAGTGC AATAAATCGC ATTTCAGGAA CCACAAGTTT 
GCTGATTTGC CATATGAGCT ACAGGACATG GTGAACAAGC ATATGAAGAG CAGCTTGCCC 
AACAAGACTC AGGGAGCTCA AGCCCAGGAT CCTGACACTC TAAGCCTGAC TCCTGCAGAT 
GTAGTGCCCA CTTCGGTCAT TGCTCGTGTT CTTGAGAAAC CCGAGCCCCT GGTGCTCAAC 
TCGGCTCAGT CTAGCAGCAG TGCAGGTCGG CCTGTGGCTG AGGATGTCTT TGTACACGTG 
GACATGACGG GACCACAAGA TGATGATGGA GGGCGGGAAA ATGGTGGTCC TGTTCCTGCT 
AGGCCTCCTG CGGTAGCTGA TACTGACCAG CAGCATGTCA ATGGCATGTG TCGAAGCCAG 
GGCAGCCTTG ATGGTCCTAC AGGAGATGAT GGTGCAGCTC CCTCTTTCGA GAAGCTGAAT 
CCTTACCCTA CTCCTTCACC ACCAAACCCG CTCTACCCAG GTCGCAAAGT GATTGAGTTT 
TCTTCTGATG ATAAAGTCAA GATCCCCAAG AACAGCCCAC TGCCCAATTG CACCTACGCT 
ACTCGTCAGG CTATTTCTTT GAGTCTTGTG CAGAATGAAG AAGAGGCAGC CGAGCGCCAG 
CGCACAGTGC CTAATAGCCC TGCCATATCT GATGGGGGTT GGCGATCTGG AACCTCCTCA 
TCTTCCGGTG GAGGGATTGC TTCTCACCAG AGGACACCTC CACAGATGGA TGCTACAGAC 
ACACTCTCCA GTCATTCAAG TCCCTTTAGC AGCCCCCCTC AGCCGCCCAG CGCCTTTGCC 
AGTTCTGGAA GCTCCGAGGA GGACCTTCTA GCCAGCTGGC AGCGCATGTT TGTTGAAAAA 
ATGGCACCTT CTTCAGATGG CTCCCTTGTC AACCGCACAT CCTTCAGCAG TGAGACGGTA 
AAAGATCTCC AAAGAAGTCA TAATGGAAAA TCAGGAGTTG GTGCTCTGAG AGGTGCTTAC 
TCTGATGGTG AGGAGGGATC CTCGGCTCAG AGCTGGACAG CAAGTAGAGA GTCAAGTTTG 
GACACAGACA CCAGCAGCAT GGCAGACCTT CGGGCAAAGA AGACACAATA TGGTGGCGAC 
TTTTCCCATG AAGAAGGTGA ACACCTTCTG ATGGCTCTGG ATCCACTTGA TAATGATGGT 
GCTAGCGGTG ACACTATGGT CACCATTGAG AGTAGTCCTG CCTCTGCGAA GCGTCAAGAG 
TTTGCAGAAG ATGTTGGATC GGCGGGAAGT TCAGCAGAAG AGCGAGATGT CCTTCCCCAG 
GACTTTCCTG TCATTGGCTC AAGGGTTCTT TCTTCCCTTG AGGATTATGC AGATAAGCCC 
CCTCGGACTG GCTCCTCGCG ACCTCTGAAG AGTCCCAAGA GGATGGGGGT TCATCACTTG 
CACCGCAAAG ACAGCCTTAC CAGAGCCCAG GAGCATGGCA ACTTGCTAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjap1 ( XM_001921650.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001921685.4 Danio rerio tight junction associated protein 1 (peripheral) (tjap1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa18386
Clone ID Related Accession (Same CDS sequence)	XM_001921650.5 , NM_001365539.1
Accession Version	XM_001921650.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2034bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction-associated protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_001921650.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGAGTG CTGCACCTGC CCGGAAACCT TACCGAAAGG CACCTCCGCA GCACCGCGAG 
ACGCGTCACA CTGTGCCAGT ATTTCGAGAG GATGTTAGCG GGAGTGCCTT GCCATCCGCC 
AACCCTGCAA CGTCAGCCCA GCCTGACCCC TGCCAGGAGT CCTTATCAGA TGCCGACAGG 
ATCAAAATCC TGCAGCAGCA AAATGAAGAC CTCCGGCGCC GACTGTCCCA GACCACACAT 
AAGATGGAGG CCATGGAGAC GGAGTTCGAA ACCAGTCGAC ATTACATGCA GGCGGAGCTT 
GGTCGAACTA GAGACGACCT GGAGAAAATG AGAGACAAGT TTAGAAGACT TCAGAACAGC 
TACACGGCAT CTCAGAGAGC TAATCAAGAC CTGGAGGAGA AACTTCACGC CCTGCTCCGT 
AAAGTGGAAA GAGACAAGAA AACTATGGAT CAAGAGATAG TGGAGCTCAC CAATAAACTT 
GTGGACGCCA AAAATACCAT CGACAAGCTG GAGGAGCTGA ACGAACGCTA TCGACAGGAT 
TGCAATCTGG CCGTGCAGCT GTTGAAGTGC AATAAATCGC ATTTCAGGAA CCACAAGTTT 
GCTGATTTGC CATATGAGCT ACAGGACATG GTGAACAAGC ATATGAAGAG CAGCTTGCCC 
AACAAGACTC AGGGAGCTCA AGCCCAGGAT CCTGACACTC TAAGCCTGAC TCCTGCAGAT 
GTAGTGCCCA CTTCGGTCAT TGCTCGTGTT CTTGAGAAAC CCGAGCCCCT GGTGCTCAAC 
TCGGCTCAGT CTAGCAGCAG TGCAGGTCGG CCTGTGGCTG AGGATGTCTT TGTACACGTG 
GACATGACGG GACCACAAGA TGATGATGGA GGGCGGGAAA ATGGTGGTCC TGTTCCTGCT 
AGGCCTCCTG CGGTAGCTGA TACTGACCAG CAGCATGTCA ATGGCATGTG TCGAAGCCAG 
GGCAGCCTTG ATGGTCCTAC AGGAGATGAT GGTGCAGCTC CCTCTTTCGA GAAGCTGAAT 
CCTTACCCTA CTCCTTCACC ACCAAACCCG CTCTACCCAG GTCGCAAAGT GATTGAGTTT 
TCTTCTGATG ATAAAGTCAA GATCCCCAAG AACAGCCCAC TGCCCAATTG CACCTACGCT 
ACTCGTCAGG CTATTTCTTT GAGTCTTGTG CAGAATGAAG AAGAGGCAGC CGAGCGCCAG 
CGCACAGTGC CTAATAGCCC TGCCATATCT GATGGGGGTT GGCGATCTGG AACCTCCTCA 
TCTTCCGGTG GAGGGATTGC TTCTCACCAG AGGACACCTC CACAGATGGA TGCTACAGAC 
ACACTCTCCA GTCATTCAAG TCCCTTTAGC AGCCCCCCTC AGCCGCCCAG CGCCTTTGCC 
AGTTCTGGAA GCTCCGAGGA GGACCTTCTA GCCAGCTGGC AGCGCATGTT TGTTGAAAAA 
ATGGCACCTT CTTCAGATGG CTCCCTTGTC AACCGCACAT CCTTCAGCAG TGAGACGGTA 
AAAGATCTCC AAAGAAGTCA TAATGGAAAA TCAGGAGTTG GTGCTCTGAG AGGTGCTTAC 
TCTGATGGTG AGGAGGGATC CTCGGCTCAG AGCTGGACAG CAAGTAGAGA GTCAAGTTTG 
GACACAGACA CCAGCAGCAT GGCAGACCTT CGGGCAAAGA AGACACAATA TGGTGGCGAC 
TTTTCCCATG AAGAAGGTGA ACACCTTCTG ATGGCTCTGG ATCCACTTGA TAATGATGGT 
GCTAGCGGTG ACACTATGGT CACCATTGAG AGTAGTCCTG CCTCTGCGAA GCGTCAAGAG 
TTTGCAGAAG ATGTTGGATC GGCGGGAAGT TCAGCAGAAG AGCGAGATGT CCTTCCCCAG 
GACTTTCCTG TCATTGGCTC AAGGGTTCTT TCTTCCCTTG AGGATTATGC AGATAAGCCC 
CCTCGGACTG GCTCCTCGCG ACCTCTGAAG AGTCCCAAGA GGATGGGGGT TCATCACTTG 
CACCGCAAAG ACAGCCTTAC CAGAGCCCAG GAGCATGGCA ACTTGCTAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001921685.4
CDS	342..2375
Translation	MTSAAPARKPYRKAPPQHRETRHTVPVFREDVSGSALPSANPATSAQPDPCQESLSDADRIKILQQQNEDLRRRLSQTTHKMEAMETEFETSRHYMQAELGRTRDDLEKMRDKFRRLQNSYTASQRANQDLEEKLHALLRKVERDKKTMDQEIVELTNKLVDAKNTIDKLEELNERYRQDCNLAVQLLKCNKSHFRNHKFADLPYELQDMVNKHMKSSLPNKTQGAQAQDPDTLSLTPADVVPTSVIARVLEKPEPLVLNSAQSSSSAGRPVAEDVFVHVDMTGPQDDDGGRENGGPVPARPPAVADTDQQHVNGMCRSQGSLDGPTGDDGAAPSFEKLNPYPTPSPPNPLYPGRKVIEFSSDDKVKIPKNSPLPNCTYATRQAISLSLVQNEEEAAERQRTVPNSPAISDGGWRSGTSSSSGGGIASHQRTPPQMDATDTLSSHSSPFSSPPQPPSAFASSGSSEEDLLASWQRMFVEKMAPSSDGSLVNRTSFSSETVKDLQRSHNGKSGVGALRGAYSDGEEGSSAQSWTASRESSLDTDTSSMADLRAKKTQYGGDFSHEEGEHLLMALDPLDNDGASGDTMVTIESSPASAKRQEFAEDVGSAGSSAEERDVLPQDFPVIGSRVLSSLEDYADKPPRTGSSRPLKSPKRMGVHHLHRKDSLTRAQEHGNLLD

Target ORF information:

RefSeq Version	XM_001921650.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction associated protein 1 (peripheral) (tjap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001921650.5

ORF Insert Sequence:

ATGACGAGTG CTGCACCTGC CCGGAAACCT TACCGAAAGG CACCTCCGCA GCACCGCGAG 
ACGCGTCACA CTGTGCCAGT ATTTCGAGAG GATGTTAGCG GGAGTGCCTT GCCATCCGCC 
AACCCTGCAA CGTCAGCCCA GCCTGACCCC TGCCAGGAGT CCTTATCAGA TGCCGACAGG 
ATCAAAATCC TGCAGCAGCA AAATGAAGAC CTCCGGCGCC GACTGTCCCA GACCACACAT 
AAGATGGAGG CCATGGAGAC GGAGTTCGAA ACCAGTCGAC ATTACATGCA GGCGGAGCTT 
GGTCGAACTA GAGACGACCT GGAGAAAATG AGAGACAAGT TTAGAAGACT TCAGAACAGC 
TACACGGCAT CTCAGAGAGC TAATCAAGAC CTGGAGGAGA AACTTCACGC CCTGCTCCGT 
AAAGTGGAAA GAGACAAGAA AACTATGGAT CAAGAGATAG TGGAGCTCAC CAATAAACTT 
GTGGACGCCA AAAATACCAT CGACAAGCTG GAGGAGCTGA ACGAACGCTA TCGACAGGAT 
TGCAATCTGG CCGTGCAGCT GTTGAAGTGC AATAAATCGC ATTTCAGGAA CCACAAGTTT 
GCTGATTTGC CATATGAGCT ACAGGACATG GTGAACAAGC ATATGAAGAG CAGCTTGCCC 
AACAAGACTC AGGGAGCTCA AGCCCAGGAT CCTGACACTC TAAGCCTGAC TCCTGCAGAT 
GTAGTGCCCA CTTCGGTCAT TGCTCGTGTT CTTGAGAAAC CCGAGCCCCT GGTGCTCAAC 
TCGGCTCAGT CTAGCAGCAG TGCAGGTCGG CCTGTGGCTG AGGATGTCTT TGTACACGTG 
GACATGACGG GACCACAAGA TGATGATGGA GGGCGGGAAA ATGGTGGTCC TGTTCCTGCT 
AGGCCTCCTG CGGTAGCTGA TACTGACCAG CAGCATGTCA ATGGCATGTG TCGAAGCCAG 
GGCAGCCTTG ATGGTCCTAC AGGAGATGAT GGTGCAGCTC CCTCTTTCGA GAAGCTGAAT 
CCTTACCCTA CTCCTTCACC ACCAAACCCG CTCTACCCAG GTCGCAAAGT GATTGAGTTT 
TCTTCTGATG ATAAAGTCAA GATCCCCAAG AACAGCCCAC TGCCCAATTG CACCTACGCT 
ACTCGTCAGG CTATTTCTTT GAGTCTTGTG CAGAATGAAG AAGAGGCAGC CGAGCGCCAG 
CGCACAGTGC CTAATAGCCC TGCCATATCT GATGGGGGTT GGCGATCTGG AACCTCCTCA 
TCTTCCGGTG GAGGGATTGC TTCTCACCAG AGGACACCTC CACAGATGGA TGCTACAGAC 
ACACTCTCCA GTCATTCAAG TCCCTTTAGC AGCCCCCCTC AGCCGCCCAG CGCCTTTGCC 
AGTTCTGGAA GCTCCGAGGA GGACCTTCTA GCCAGCTGGC AGCGCATGTT TGTTGAAAAA 
ATGGCACCTT CTTCAGATGG CTCCCTTGTC AACCGCACAT CCTTCAGCAG TGAGACGGTA 
AAAGATCTCC AAAGAAGTCA TAATGGAAAA TCAGGAGTTG GTGCTCTGAG AGGTGCTTAC 
TCTGATGGTG AGGAGGGATC CTCGGCTCAG AGCTGGACAG CAAGTAGAGA GTCAAGTTTG 
GACACAGACA CCAGCAGCAT GGCAGACCTT CGGGCAAAGA AGACACAATA TGGTGGCGAC 
TTTTCCCATG AAGAAGGTGA ACACCTTCTG ATGGCTCTGG ATCCACTTGA TAATGATGGT 
GCTAGCGGTG ACACTATGGT CACCATTGAG AGTAGTCCTG CCTCTGCGAA GCGTCAAGAG 
TTTGCAGAAG ATGTTGGATC GGCGGGAAGT TCAGCAGAAG AGCGAGATGT CCTTCCCCAG 
GACTTTCCTG TCATTGGCTC AAGGGTTCTT TCTTCCCTTG AGGATTATGC AGATAAGCCC 
CCTCGGACTG GCTCCTCGCG ACCTCTGAAG AGTCCCAAGA GGATGGGGGT TCATCACTTG 
CACCGCAAAG ACAGCCTTAC CAGAGCCCAG GAGCATGGCA ACTTGCTAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjap1 ( XM_009307071.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009305346.1 Danio rerio tight junction associated protein 1 (peripheral) (tjap1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa44781
Clone ID Related Accession (Same CDS sequence)	XM_009307071.1
Accession Version	XM_009307071.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tight junction-associated protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACGAGTG CTGCACCTGC CCGGAAACCT TACCGAAAGG CACCTCCGCA GCACCGCGAG 
ACGCGTCACA CTGTGCCAGT ATTTCGAGAG GATGTTAGCG GGAGTGCCTT GCCATCCGCC 
AACCCTGCAA CGTCAGCCCA GCCTGACCCC TGCCAGGAGT CCTTATCAGA TGCCGACAGG 
ATCAAAATCC TGCAGCAGCA AAATGAAGAC CTCCGGCGCC GACTGTCCCA GACCACACAT 
AAGATGGAGG CCATGGAGAC GGAGTTCGAA ACCAGTCGAC ATTACATGCA GGCGGAGCTT 
GGTCGAACTA GAGACGACCT GGAGAAAATG AGAGACAAGT TTAGAAGACT TCAGAACAGC 
TACACGGCAT CTCAGAGAGC TAATCAAGAC CTGGAGGAGA AACTTCACGC CCTGGCTGCC 
ATCAGTCAAA CTTGGGTCTA TGCACTCCGT AAAGTGGAAA GAGACAAGAA AACTATGGAT 
CAAGAGATAG TGGAGCTCAC CAATAAACTT GTGGACGCCA AAAATACCAT CGACAAGCTG 
GAGGAGCTGA ACGAACGCTA TCGACAGGAT TGCAATCTGG CCGTGCAGCT GTTGAAGTGC 
AATAAATCGC ATTTCAGGAA CCACAAGTTT GCTGATTTGC CATATGAGCT ACAGGACATG 
GTGAACAAGC ATATGAAGAG CAGCTTGCCC AACAAGACTC AGGGAGCTCA AGCCCAGGAT 
CCTGACACTC TAAGCCTGAC TCCTGCAGAT GTAGTGCCCA CTTCGGTCAT TGCTCGTGTT 
CTTGAGAAAC CCGAGCCCCT GGTGCTCAAC TCGGCTCAGT CTAGCAGCAG TGCAGGTCGG 
CCTGTGGCTG AGGATGTCTT TGTACACGTG GACATGACGG GACCACAAGA TGATGATGGA 
GGGCGGGAAA ATGGTGGTCC TGTTCCTGCT AGGCCTCCTG CGGTAGCTGA TACTGACCAG 
CAGCATGTCA ATGGCATGTG TCGAAGCCAG GGCAGCCTTG ATGGTCCTAC AGGAGATGAT 
GGTGCAGCTC CCTCTTTCGA GAAGCTGAAT CCTTACCCTA CTCCTTCACC ACCAAACCCG 
CTCTACCCAG GTCGCAAAGT GATTGAGTTT TCTTCTGATG ATAAAGTCAA GATCCCCAAG 
AACAGCCCAC TGCCCAATTG CACCTACGCT ACTCGTCAGG CTATTTCTTT GAGTCTTGTG 
CAGAATGAAG AAGAGGCAGC CGAGCGCCAG CGCACAGTGC CTAATAGCCC TGCCATATCT 
GATGGGGGTT GGCGATCTGG AACCTCCTCA TCTTCCGGTG GAGGGATTGC TTCTCACCAG 
AGGACACCTC CACAGATGGA TGCTACAGAC ACACTCTCCA GTCATTCAAG TCCCTTTAGC 
AGCCCCCCTC AGCCGCCCAG CGCCTTTGCC AGTTCTGGAA GCTCCGAGGA GGACCTTCTA 
GCCAGCTGGC AGCGCATGTT TGTTGAAAAA ATGGCACCTT CTTCAGATGG CTCCCTTGTC 
AACCGCACAT CCTTCAGCAG TGAGACGGTA AAAGATCTCC AAAGAAGTCA TAATGGAAAA 
TCAGGAGTTG GTGCTCTGAG AGGTGCTTAC TCTGATGGTG AGGAGGGATC CTCGGCTCAG 
AGCTGGACAG CAAGTAGAGA GTCAAGTTTG GACACAGACA CCAGCAGCAT GGCAGACCTT 
CGGGCAAAGA AGACACAATA TGGTGGCGAC TTTTCCCATG AAGAAGGTGA ACACCTTCTG 
ATGGCTCTGG ATCCACTTGA TAATGATGGT GCTAGCGGTG ACACTATGGT CACCATTGAG 
AGTAGTCCTG CCTCTGCGAA GCGTCAAGAG TTTGCAGAAG ATGTTGGATC GGCGGGAAGT 
TCAGCAGAAG AGCGAGATGT CCTTCCCCAG GACTTTCCTG TCATTGGCTC AAGGGTTCTT 
TCTTCCCTTG AGGATTATGC AGATAAGCCC CCTCGGACTG GCTCCTCGCG ACCTCTGAAG 
AGTCCCAAGA GGATGGGGGT TCATCACTTG CACCGCAAAG ACAGCCTTAC CAGAGCCCAG 
GAGCATGGCA ACTTGCTAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305346.1
CDS	342..2405
Translation	MTSAAPARKPYRKAPPQHRETRHTVPVFREDVSGSALPSANPATSAQPDPCQESLSDADRIKILQQQNEDLRRRLSQTTHKMEAMETEFETSRHYMQAELGRTRDDLEKMRDKFRRLQNSYTASQRANQDLEEKLHALAAISQTWVYALRKVERDKKTMDQEIVELTNKLVDAKNTIDKLEELNERYRQDCNLAVQLLKCNKSHFRNHKFADLPYELQDMVNKHMKSSLPNKTQGAQAQDPDTLSLTPADVVPTSVIARVLEKPEPLVLNSAQSSSSAGRPVAEDVFVHVDMTGPQDDDGGRENGGPVPARPPAVADTDQQHVNGMCRSQGSLDGPTGDDGAAPSFEKLNPYPTPSPPNPLYPGRKVIEFSSDDKVKIPKNSPLPNCTYATRQAISLSLVQNEEEAAERQRTVPNSPAISDGGWRSGTSSSSGGGIASHQRTPPQMDATDTLSSHSSPFSSPPQPPSAFASSGSSEEDLLASWQRMFVEKMAPSSDGSLVNRTSFSSETVKDLQRSHNGKSGVGALRGAYSDGEEGSSAQSWTASRESSLDTDTSSMADLRAKKTQYGGDFSHEEGEHLLMALDPLDNDGASGDTMVTIESSPASAKRQEFAEDVGSAGSSAEERDVLPQDFPVIGSRVLSSLEDYADKPPRTGSSRPLKSPKRMGVHHLHRKDSLTRAQEHGNLLD

Target ORF information:

RefSeq Version	XM_009307071.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tight junction associated protein 1 (peripheral) (tjap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009307071.1

ORF Insert Sequence:

ATGACGAGTG CTGCACCTGC CCGGAAACCT TACCGAAAGG CACCTCCGCA GCACCGCGAG 
ACGCGTCACA CTGTGCCAGT ATTTCGAGAG GATGTTAGCG GGAGTGCCTT GCCATCCGCC 
AACCCTGCAA CGTCAGCCCA GCCTGACCCC TGCCAGGAGT CCTTATCAGA TGCCGACAGG 
ATCAAAATCC TGCAGCAGCA AAATGAAGAC CTCCGGCGCC GACTGTCCCA GACCACACAT 
AAGATGGAGG CCATGGAGAC GGAGTTCGAA ACCAGTCGAC ATTACATGCA GGCGGAGCTT 
GGTCGAACTA GAGACGACCT GGAGAAAATG AGAGACAAGT TTAGAAGACT TCAGAACAGC 
TACACGGCAT CTCAGAGAGC TAATCAAGAC CTGGAGGAGA AACTTCACGC CCTGGCTGCC 
ATCAGTCAAA CTTGGGTCTA TGCACTCCGT AAAGTGGAAA GAGACAAGAA AACTATGGAT 
CAAGAGATAG TGGAGCTCAC CAATAAACTT GTGGACGCCA AAAATACCAT CGACAAGCTG 
GAGGAGCTGA ACGAACGCTA TCGACAGGAT TGCAATCTGG CCGTGCAGCT GTTGAAGTGC 
AATAAATCGC ATTTCAGGAA CCACAAGTTT GCTGATTTGC CATATGAGCT ACAGGACATG 
GTGAACAAGC ATATGAAGAG CAGCTTGCCC AACAAGACTC AGGGAGCTCA AGCCCAGGAT 
CCTGACACTC TAAGCCTGAC TCCTGCAGAT GTAGTGCCCA CTTCGGTCAT TGCTCGTGTT 
CTTGAGAAAC CCGAGCCCCT GGTGCTCAAC TCGGCTCAGT CTAGCAGCAG TGCAGGTCGG 
CCTGTGGCTG AGGATGTCTT TGTACACGTG GACATGACGG GACCACAAGA TGATGATGGA 
GGGCGGGAAA ATGGTGGTCC TGTTCCTGCT AGGCCTCCTG CGGTAGCTGA TACTGACCAG 
CAGCATGTCA ATGGCATGTG TCGAAGCCAG GGCAGCCTTG ATGGTCCTAC AGGAGATGAT 
GGTGCAGCTC CCTCTTTCGA GAAGCTGAAT CCTTACCCTA CTCCTTCACC ACCAAACCCG 
CTCTACCCAG GTCGCAAAGT GATTGAGTTT TCTTCTGATG ATAAAGTCAA GATCCCCAAG 
AACAGCCCAC TGCCCAATTG CACCTACGCT ACTCGTCAGG CTATTTCTTT GAGTCTTGTG 
CAGAATGAAG AAGAGGCAGC CGAGCGCCAG CGCACAGTGC CTAATAGCCC TGCCATATCT 
GATGGGGGTT GGCGATCTGG AACCTCCTCA TCTTCCGGTG GAGGGATTGC TTCTCACCAG 
AGGACACCTC CACAGATGGA TGCTACAGAC ACACTCTCCA GTCATTCAAG TCCCTTTAGC 
AGCCCCCCTC AGCCGCCCAG CGCCTTTGCC AGTTCTGGAA GCTCCGAGGA GGACCTTCTA 
GCCAGCTGGC AGCGCATGTT TGTTGAAAAA ATGGCACCTT CTTCAGATGG CTCCCTTGTC 
AACCGCACAT CCTTCAGCAG TGAGACGGTA AAAGATCTCC AAAGAAGTCA TAATGGAAAA 
TCAGGAGTTG GTGCTCTGAG AGGTGCTTAC TCTGATGGTG AGGAGGGATC CTCGGCTCAG 
AGCTGGACAG CAAGTAGAGA GTCAAGTTTG GACACAGACA CCAGCAGCAT GGCAGACCTT 
CGGGCAAAGA AGACACAATA TGGTGGCGAC TTTTCCCATG AAGAAGGTGA ACACCTTCTG 
ATGGCTCTGG ATCCACTTGA TAATGATGGT GCTAGCGGTG ACACTATGGT CACCATTGAG 
AGTAGTCCTG CCTCTGCGAA GCGTCAAGAG TTTGCAGAAG ATGTTGGATC GGCGGGAAGT 
TCAGCAGAAG AGCGAGATGT CCTTCCCCAG GACTTTCCTG TCATTGGCTC AAGGGTTCTT 
TCTTCCCTTG AGGATTATGC AGATAAGCCC CCTCGGACTG GCTCCTCGCG ACCTCTGAAG 
AGTCCCAAGA GGATGGGGGT TCATCACTTG CACCGCAAAG ACAGCCTTAC CAGAGCCCAG 
GAGCATGGCA ACTTGCTAGA CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tjap1 ( XM_021480930.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021336605.1 Danio rerio tight junction associated protein 1 (peripheral) (tjap1), mRNA.

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CloneID	ODa148685
Clone ID Related Accession (Same CDS sequence)	XM_021480930.1
Accession Version	XM_021480930.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3300bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tight junction-associated protein 1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

ATGACGAGTG CTGCACCTGC CCGGAAACCT TACCGAAAGG CACCTCCGCA GCACCGCGAG 
ACGCGTCACA CTGTGCCAGT ATTTCGAGAG GATGTTAGCG GGAGTGCCTT GCCATCCGCC 
AACCCTGCAA CGTCAGCCCA GCCTGACCCC TGCCAGGTAA TTTTCCCCCG AAATCATGCA 
CCTGTTTTGC AGCGTACACG GTTTACAGGC CCTCATCCGC AGAGTCCTGA GAGATCTTCA 
TGGTCGCAAG CATCCGACAG CGAACTGGAA GTCTCAAGCC TTTCTAGCCT AGAACTCAGT 
GTTCTCCCTC CACCACGGAT CTTCAGCCAC AGTTCTCTTG TTATGAGACT TCCTCCCAAG 
CATCTTCGAC AACAAAAAAT GAACCGTTTC ATCAGTCATA GTGATGACCT GCTGGTGTTT 
TCTGCTGATG ATGATCTTGC CTCCTCGAGC AGTTCCCAGC AGTGCATCCG AGGCAGCTCT 
CCATCACATT CCAGCAGAAG TGCGGAGCGT TCCCTGGTCT TCAAAGAGAA AGCTGAAATA 
CTTGTTCCGA GAGAAGAGAA GCGTCCACCG CTTCCGGCAT CTCAGATGCA TGTCCATCCA 
CCCAAGGGCA TTCTTAAGCA GTCTCACCAG TTGGGAGTTT GCCTTTCTGA CAGTCTGCGC 
AAGTCTAAGT CGGCAGAAAT GTTAGACAAG GCACAAAGTC GTGGAAGAAG CAGGAAGCCC 
AAAAGCATGT CCTTGGACAG GGAGAAGGGG GTACGAGTCT CTCCCATGGG ACATGGAACT 
TCCACATCAT CTATATCTCC AAGCTCCATG CTACCAGACT GGAAAATGCA GCGGCTTGAG 
GAAAAGGTGA AATTTTCACA GTTTGTAGAT GAAATCACCT ACCGGGTCCT AAGCCCTGCC 
AATCTAAAGA TGTTGCGCGG GAACCTTCCA AGGTCCACAC AGACCAAGCA GCACAATCCT 
CAACAGTATG AATTCAAAGA CCATCATGAG AAAAAACAAG AAAGGTGCAG ACCTTGGGAT 
GAGTGGGCCA CTCAGCATAG CAGAACAAGC AGACAATCAA AGGAAGGAAG ACCCAAGACA 
TGGCACATCC CGGGTGATAG TCCAGAGAGA GTGCCAGAAA AGCAGCATGT TGATCCCCAT 
CGGGAAGGAG CCCGACCTAA AGCTGAAGGC CCAATTTACA GTCGGGTGGG GGGCAGCGAT 
GATTCCGAAT GGGATGACAG GACTCCACTG GGGGATCAGC TCCAGGTTCC CCATATAGAG 
AACCGAACCC TGCAGAAGGA CAGTGTAGAC AAATCAGGGT TGCCAAAGGG TGAGATTCCC 
ACTATTCGTG TGGAGAGTGA GAAAACGTCT TTATCACAAG TGACTGCCTT TACTATATTA 
TCACAAATCA AGGAGTCCTT ATCAGATGCC GACAGGATCA AAATCCTGCA GCAGCAAAAT 
GAAGACCTCC GGCGCCGACT GTCCCAGACC ACACATAAGA TGGAGGCCAT GGAGACGGAG 
TTCGAAACCA GTCGACATTA CATGCAGGCG GAGCTTGGTC GAACTAGAGA CGACCTGGAG 
AAAATGAGAG ACAAGTTTAG AAGACTTCAG AACAGCTACA CGGCATCTCA GAGAGCTAAT 
CAAGACCTGG AGGAGAAACT TCACGCCCTG GCTGCCATCA GTCAAACTTG GGTCTATGCA 
CTCCGTAAAG TGGAAAGAGA CAAGAAAACT ATGGATCAAG AGATAGTGGA GCTCACCAAT 
AAACTTGTGG ACGCCAAAAA TACCATCGAC AAGCTGGAGG AGCTGAACGA ACGCTATCGA 
CAGGATTGCA ATCTGGCCGT GCAGCTGTTG AAGTGCAATA AATCGCATTT CAGGAACCAC 
AAGTTTGCTG ATTTGCCATA TGAGCTACAG GACATGGTGA ACAAGCATAT GAAGAGCAGC 
TTGCCCAACA AGACTCAGGG AGCTCAAGCC CAGGATCCTG ACACTCTAAG CCTGACTCCT 
GCAGATGTAG TGCCCACTTC GGTCATTGCT CGTGTTCTTG AGAAACCCGA GCCCCTGGTG 
CTCAACTCGG CTCAGTCTAG CAGCAGTGCA GGTCGGCCTG TGGCTGAGGA TGTCTTTGTA 
CACGTGGACA TGACGGGACC ACAAGATGAT GATGGAGGGC GGGAAAATGG TGGTCCTGTT 
CCTGCTAGGC CTCCTGCGGT AGCTGATACT GACCAGCAGC ATGTCAATGG CATGTGTCGA 
AGCCAGGGCA GCCTTGATGG TCCTACAGGA GATGATGGTG CAGCTCCCTC TTTCGAGAAG 
CTGAATCCTT ACCCTACTCC TTCACCACCA AACCCGCTCT ACCCAGGTCG CAAAGTGATT 
GAGTTTTCTT CTGATGATAA AGTCAAGATC CCCAAGAACA GCCCACTGCC CAATTGCACC 
TACGCTACTC GTCAGGCTAT TTCTTTGAGT CTTGTGCAGA ATGAAGAAGA GGCAGCCGAG 
CGCCAGCGCA CAGTGCCTAA TAGCCCTGCC ATATCTGATG GGGGTTGGCG ATCTGGAACC 
TCCTCATCTT CCGGTGGAGG GATTGCTTCT CACCAGAGGA CACCTCCACA GATGGATGCT 
ACAGACACAC TCTCCAGTCA TTCAAGTCCC TTTAGCAGCC CCCCTCAGCC GCCCAGCGCC 
TTTGCCAGTT CTGGAAGCTC CGAGGAGGAC CTTCTAGCCA GCTGGCAGCG CATGTTTGTT 
GAAAAAATGG CACCTTCTTC AGATGGCTCC CTTGTCAACC GCACATCCTT CAGCAGTGAG 
ACGGTAAAAG ATCTCCAAAG AAGTCATAAT GGAAAATCAG GAGTTGGTGC TCTGAGAGGT 
GCTTACTCTG ATGGTGAGGA GGGATCCTCG GCTCAGAGCT GGACAGCAAG TAGAGAGTCA 
AGTTTGGACA CAGACACCAG CAGCATGGCA GACCTTCGGG CAAAGAAGAC ACAATATGGT 
GGCGACTTTT CCCATGAAGA AGGTGAACAC CTTCTGATGG CTCTGGATCC ACTTGATAAT 
GATGGTGCTA GCGGTGACAC TATGGTCACC ATTGAGAGTA GTCCTGCCTC TGCGAAGCGT 
CAAGAGTTTG CAGAAGATGT TGGATCGGCG GGAAGTTCAG CAGAAGAGCG AGATGTCCTT 
CCCCAGGACT TTCCTGTCAT TGGCTCAAGG GTTCTTTCTT CCCTTGAGGA TTATGCAGAT 
AAGCCCCCTC GGACTGGCTC CTCGCGACCT CTGAAGAGTC CCAAGAGGAT GGGGGTTCAT 
CACTTGCACC GCAAAGACAG CCTTACCAGA GCCCAGGAGC ATGGCAACTT GCTAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021336605.1
CDS	1..3300
Translation	MTSAAPARKPYRKAPPQHRETRHTVPVFREDVSGSALPSANPATSAQPDPCQVIFPRNHAPVLQRTRFTGPHPQSPERSSWSQASDSELEVSSLSSLELSVLPPPRIFSHSSLVMRLPPKHLRQQKMNRFISHSDDLLVFSADDDLASSSSSQQCIRGSSPSHSSRSAERSLVFKEKAEILVPREEKRPPLPASQMHVHPPKGILKQSHQLGVCLSDSLRKSKSAEMLDKAQSRGRSRKPKSMSLDREKGVRVSPMGHGTSTSSISPSSMLPDWKMQRLEEKVKFSQFVDEITYRVLSPANLKMLRGNLPRSTQTKQHNPQQYEFKDHHEKKQERCRPWDEWATQHSRTSRQSKEGRPKTWHIPGDSPERVPEKQHVDPHREGARPKAEGPIYSRVGGSDDSEWDDRTPLGDQLQVPHIENRTLQKDSVDKSGLPKGEIPTIRVESEKTSLSQVTAFTILSQIKESLSDADRIKILQQQNEDLRRRLSQTTHKMEAMETEFETSRHYMQAELGRTRDDLEKMRDKFRRLQNSYTASQRANQDLEEKLHALAAISQTWVYALRKVERDKKTMDQEIVELTNKLVDAKNTIDKLEELNERYRQDCNLAVQLLKCNKSHFRNHKFADLPYELQDMVNKHMKSSLPNKTQGAQAQDPDTLSLTPADVVPTSVIARVLEKPEPLVLNSAQSSSSAGRPVAEDVFVHVDMTGPQDDDGGRENGGPVPARPPAVADTDQQHVNGMCRSQGSLDGPTGDDGAAPSFEKLNPYPTPSPPNPLYPGRKVIEFSSDDKVKIPKNSPLPNCTYATRQAISLSLVQNEEEAAERQRTVPNSPAISDGGWRSGTSSSSGGGIASHQRTPPQMDATDTLSSHSSPFSSPPQPPSAFASSGSSEEDLLASWQRMFVEKMAPSSDGSLVNRTSFSSETVKDLQRSHNGKSGVGALRGAYSDGEEGSSAQSWTASRESSLDTDTSSMADLRAKKTQYGGDFSHEEGEHLLMALDPLDNDGASGDTMVTIESSPASAKRQEFAEDVGSAGSSAEERDVLPQDFPVIGSRVLSSLEDYADKPPRTGSSRPLKSPKRMGVHHLHRKDSLTRAQEHGNLLD

Target ORF information:

RefSeq Version	XM_021480930.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tight junction associated protein 1 (peripheral) (tjap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480930.1

ORF Insert Sequence:

ATGACGAGTG CTGCACCTGC CCGGAAACCT TACCGAAAGG CACCTCCGCA GCACCGCGAG 
ACGCGTCACA CTGTGCCAGT ATTTCGAGAG GATGTTAGCG GGAGTGCCTT GCCATCCGCC 
AACCCTGCAA CGTCAGCCCA GCCTGACCCC TGCCAGGTAA TTTTCCCCCG AAATCATGCA 
CCTGTTTTGC AGCGTACACG GTTTACAGGC CCTCATCCGC AGAGTCCTGA GAGATCTTCA 
TGGTCGCAAG CATCCGACAG CGAACTGGAA GTCTCAAGCC TTTCTAGCCT AGAACTCAGT 
GTTCTCCCTC CACCACGGAT CTTCAGCCAC AGTTCTCTTG TTATGAGACT TCCTCCCAAG 
CATCTTCGAC AACAAAAAAT GAACCGTTTC ATCAGTCATA GTGATGACCT GCTGGTGTTT 
TCTGCTGATG ATGATCTTGC CTCCTCGAGC AGTTCCCAGC AGTGCATCCG AGGCAGCTCT 
CCATCACATT CCAGCAGAAG TGCGGAGCGT TCCCTGGTCT TCAAAGAGAA AGCTGAAATA 
CTTGTTCCGA GAGAAGAGAA GCGTCCACCG CTTCCGGCAT CTCAGATGCA TGTCCATCCA 
CCCAAGGGCA TTCTTAAGCA GTCTCACCAG TTGGGAGTTT GCCTTTCTGA CAGTCTGCGC 
AAGTCTAAGT CGGCAGAAAT GTTAGACAAG GCACAAAGTC GTGGAAGAAG CAGGAAGCCC 
AAAAGCATGT CCTTGGACAG GGAGAAGGGG GTACGAGTCT CTCCCATGGG ACATGGAACT 
TCCACATCAT CTATATCTCC AAGCTCCATG CTACCAGACT GGAAAATGCA GCGGCTTGAG 
GAAAAGGTGA AATTTTCACA GTTTGTAGAT GAAATCACCT ACCGGGTCCT AAGCCCTGCC 
AATCTAAAGA TGTTGCGCGG GAACCTTCCA AGGTCCACAC AGACCAAGCA GCACAATCCT 
CAACAGTATG AATTCAAAGA CCATCATGAG AAAAAACAAG AAAGGTGCAG ACCTTGGGAT 
GAGTGGGCCA CTCAGCATAG CAGAACAAGC AGACAATCAA AGGAAGGAAG ACCCAAGACA 
TGGCACATCC CGGGTGATAG TCCAGAGAGA GTGCCAGAAA AGCAGCATGT TGATCCCCAT 
CGGGAAGGAG CCCGACCTAA AGCTGAAGGC CCAATTTACA GTCGGGTGGG GGGCAGCGAT 
GATTCCGAAT GGGATGACAG GACTCCACTG GGGGATCAGC TCCAGGTTCC CCATATAGAG 
AACCGAACCC TGCAGAAGGA CAGTGTAGAC AAATCAGGGT TGCCAAAGGG TGAGATTCCC 
ACTATTCGTG TGGAGAGTGA GAAAACGTCT TTATCACAAG TGACTGCCTT TACTATATTA 
TCACAAATCA AGGAGTCCTT ATCAGATGCC GACAGGATCA AAATCCTGCA GCAGCAAAAT 
GAAGACCTCC GGCGCCGACT GTCCCAGACC ACACATAAGA TGGAGGCCAT GGAGACGGAG 
TTCGAAACCA GTCGACATTA CATGCAGGCG GAGCTTGGTC GAACTAGAGA CGACCTGGAG 
AAAATGAGAG ACAAGTTTAG AAGACTTCAG AACAGCTACA CGGCATCTCA GAGAGCTAAT 
CAAGACCTGG AGGAGAAACT TCACGCCCTG GCTGCCATCA GTCAAACTTG GGTCTATGCA 
CTCCGTAAAG TGGAAAGAGA CAAGAAAACT ATGGATCAAG AGATAGTGGA GCTCACCAAT 
AAACTTGTGG ACGCCAAAAA TACCATCGAC AAGCTGGAGG AGCTGAACGA ACGCTATCGA 
CAGGATTGCA ATCTGGCCGT GCAGCTGTTG AAGTGCAATA AATCGCATTT CAGGAACCAC 
AAGTTTGCTG ATTTGCCATA TGAGCTACAG GACATGGTGA ACAAGCATAT GAAGAGCAGC 
TTGCCCAACA AGACTCAGGG AGCTCAAGCC CAGGATCCTG ACACTCTAAG CCTGACTCCT 
GCAGATGTAG TGCCCACTTC GGTCATTGCT CGTGTTCTTG AGAAACCCGA GCCCCTGGTG 
CTCAACTCGG CTCAGTCTAG CAGCAGTGCA GGTCGGCCTG TGGCTGAGGA TGTCTTTGTA 
CACGTGGACA TGACGGGACC ACAAGATGAT GATGGAGGGC GGGAAAATGG TGGTCCTGTT 
CCTGCTAGGC CTCCTGCGGT AGCTGATACT GACCAGCAGC ATGTCAATGG CATGTGTCGA 
AGCCAGGGCA GCCTTGATGG TCCTACAGGA GATGATGGTG CAGCTCCCTC TTTCGAGAAG 
CTGAATCCTT ACCCTACTCC TTCACCACCA AACCCGCTCT ACCCAGGTCG CAAAGTGATT 
GAGTTTTCTT CTGATGATAA AGTCAAGATC CCCAAGAACA GCCCACTGCC CAATTGCACC 
TACGCTACTC GTCAGGCTAT TTCTTTGAGT CTTGTGCAGA ATGAAGAAGA GGCAGCCGAG 
CGCCAGCGCA CAGTGCCTAA TAGCCCTGCC ATATCTGATG GGGGTTGGCG ATCTGGAACC 
TCCTCATCTT CCGGTGGAGG GATTGCTTCT CACCAGAGGA CACCTCCACA GATGGATGCT 
ACAGACACAC TCTCCAGTCA TTCAAGTCCC TTTAGCAGCC CCCCTCAGCC GCCCAGCGCC 
TTTGCCAGTT CTGGAAGCTC CGAGGAGGAC CTTCTAGCCA GCTGGCAGCG CATGTTTGTT 
GAAAAAATGG CACCTTCTTC AGATGGCTCC CTTGTCAACC GCACATCCTT CAGCAGTGAG 
ACGGTAAAAG ATCTCCAAAG AAGTCATAAT GGAAAATCAG GAGTTGGTGC TCTGAGAGGT 
GCTTACTCTG ATGGTGAGGA GGGATCCTCG GCTCAGAGCT GGACAGCAAG TAGAGAGTCA 
AGTTTGGACA CAGACACCAG CAGCATGGCA GACCTTCGGG CAAAGAAGAC ACAATATGGT 
GGCGACTTTT CCCATGAAGA AGGTGAACAC CTTCTGATGG CTCTGGATCC ACTTGATAAT 
GATGGTGCTA GCGGTGACAC TATGGTCACC ATTGAGAGTA GTCCTGCCTC TGCGAAGCGT 
CAAGAGTTTG CAGAAGATGT TGGATCGGCG GGAAGTTCAG CAGAAGAGCG AGATGTCCTT 
CCCCAGGACT TTCCTGTCAT TGGCTCAAGG GTTCTTTCTT CCCTTGAGGA TTATGCAGAT 
AAGCCCCCTC GGACTGGCTC CTCGCGACCT CTGAAGAGTC CCAAGAGGAT GGGGGTTCAT 
CACTTGCACC GCAAAGACAG CCTTACCAGA GCCCAGGAGC ATGGCAACTT GCTAGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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tjap1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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