trpm3 gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_009301557.3	XP_009299832.1	transient receptor potential cation channel subfamily M member 3 isoform X8
XM_005171928.4	XP_005171985.1	transient receptor potential cation channel subfamily M member 3 isoform X12
XM_005171927.3	XP_005171984.1	transient receptor potential cation channel subfamily M member 3 isoform X6
XM_689075.7	XP_694167.5	transient receptor potential cation channel subfamily M member 3 isoform X8
XM_009301563.2	XP_009299838.1	transient receptor potential cation channel subfamily M member 3 isoform X11
XM_009301557.2	XP_009299832.1	transient receptor potential cation channel subfamily M member 3 isoform X1
XM_009301558.2	XP_009299833.1	transient receptor potential cation channel subfamily M member 3 isoform X2
XM_009301562.2	XP_009299837.1	transient receptor potential cation channel subfamily M member 3 isoform X10
XM_021476474.1	XP_021332149.1	transient receptor potential cation channel subfamily M member 3 isoform X9
XM_005171931.4	XP_005171988.1	transient receptor potential cation channel subfamily M member 3 isoform X13
XM_009301561.3	XP_009299836.1	transient receptor potential cation channel subfamily M member 3 isoform X15
XM_009301560.2	XP_009299835.1	transient receptor potential cation channel subfamily M member 3 isoform X4
XM_021476467.1	XP_021332142.1	transient receptor potential cation channel subfamily M member 3 isoform X1
XM_021476468.1	XP_021332143.1	transient receptor potential cation channel subfamily M member 3 isoform X2
XM_021476470.1	XP_021332145.1	transient receptor potential cation channel subfamily M member 3 isoform X4
XM_021476471.1	XP_021332146.1	transient receptor potential cation channel subfamily M member 3 isoform X5
XM_021476472.1	XP_021332147.1	transient receptor potential cation channel subfamily M member 3 isoform X6
XM_021476473.1	XP_021332148.1	transient receptor potential cation channel subfamily M member 3 isoform X7
XM_005171929.3	XP_005171986.1	transient receptor potential cation channel subfamily M member 3 isoform X3
XM_005171928.3	XP_005171985.1	transient receptor potential cation channel subfamily M member 3 isoform X5
XM_005171931.3	XP_005171988.1	transient receptor potential cation channel subfamily M member 3 isoform X7
XM_009301561.2	XP_009299836.1	transient receptor potential cation channel subfamily M member 3 isoform X9
XM_021476469.1	XP_021332144.1	transient receptor potential cation channel subfamily M member 3 isoform X3
XM_009301558.3	XP_009299833.1	transient receptor potential cation channel subfamily M member 3 isoform X10
XM_005171929.4	XP_005171986.1	transient receptor potential cation channel subfamily M member 3 isoform X11
XM_689075.8	XP_694167.5	transient receptor potential cation channel subfamily M member 3 isoform X14

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa44722	XM_009301562.2 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa44723	XM_009301563.2 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa142786	XM_021476467.1 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142787	XM_021476468.1 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142788	XM_021476469.1 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142789	XM_021476470.1 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142790	XM_021476471.1 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142791	XM_021476472.1 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142792	XM_021476473.1 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142793	XM_021476474.1 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa10542	XM_005171927.3 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa10543	XM_005171928.4 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa10544	XM_005171929.4 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa10546	XM_689075.8 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa10547	XM_005171931.4 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa44718	XM_009301557.3 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa44719	XM_009301558.3 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa44720	XM_009301560.2 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa44721	XM_009301561.3 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
ODa44718	XM_009301557.2	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa10543	XM_005171928.3	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa10547	XM_005171931.3	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa44721	XM_009301561.2	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa44719	XM_009301558.2	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa10544	XM_005171929.3	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa10546	XM_689075.7	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

trpm3 ( XM_009301562.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299837.1 Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X10, mRNA.

Price & Availability↑

CloneID	ODa44722
Clone ID Related Accession (Same CDS sequence)	XM_009301562.2
Accession Version	XM_009301562.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transient receptor potential cation channel subfamily M member 3 isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301562.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAACTCA GGATCACAGT GTTTCGGATG GGATCAGAGG GACACCAGGA CATCGATCTA 
GCCATTCTAA CAGCTCTTCT GAAAGGAGCG AATGCATCAG CTCCAGATCA GCTAAGTCTG 
GCTTTGGCCT GGAACAGAGT TGACATCGCC CGGAGCCAGA TCTTCATTTA CGGACAGCAG 
TGGCCTGTTG GCTCTCTGGA GCAGTCTATG CTGGACGCGC TGGTGCTGGA CCGAGTGGAC 
TTTGTAAAGC TGTTGATTGA GAACGGAGTG AGCATGCACC GTTTCCTGAC ACTCTCCAGA 
CTTGAAGAGC TTTATAATAC GAGGCACGGA CCATCCAACA CCTTGTACCA CCTTGTCAGA 
GATGTGAAAA AGCAAGAATA TCCAGGGTTC AGTTGGATCT ACCTCAAGGG TAACCTGCCC 
CCCGACTACC GCATTAGCCT GATTGACATC GGATTGGTTA TTGAATACCT CATGGGTGGA 
GCCTATCGCT GCAATTACAC CAGGAAGAGA TTTCGGACCC TCTATCACAA CCTCTTTGGA 
CCCAAAAGAC CAAAAGCCCT GAAGCTGCTT GGAATGGAGG ATGACATGCC CATTAGACGA 
GGGCGTCAGA AGACTACGCG GAAGCGAGAG GAGGAAGTAG ATATTGACCT GGATGACCCA 
GAAATCAATC ATTTCCCTTT CCCATTCCAT GAGCTGATGG TTTGGGCTGT GCTGATGAAA 
CGGCAGAAGA TGGCACTGTT CTTCTGGCAG CATGGAGAGG AGGCCATGGC TAAAGCGCTG 
GTGGCCTGCA AGCTCTGTAA GGCAATGGCT CACGAAGCTT CTGAGAACGA CATGGTCGAT 
GACATTTCAC AGGAGCTCAA TCAAAACTCA AGGGAGTTTG GTCAGCTGGC TGTTGAGCTG 
CTCGATCAGT CCTATAAGCA GGATGAACAG ATGGCTATGA AGCTGTTGAC CTACGAGCTG 
AAGAACTGGA GTAATGCCAC CTGTCTGCAG CTGGCAGTGG CTGCCAAGCA CCGAGACTTC 
ATCGCCCACA CCTGTAGCCA GATGCTGCTC ACAGACATGT GGATGGGACG TCTGCGCATG 
CGCAAGAACT CTGGCCTGAA GGTTATCTTG GGCCTGCTTC TCCCCCCTTC CATATTGAGT 
CTGGAGTTTA AAAATAAAGA CGAGATGTCA TACATGCCTC AGGATCAGGA CACATATCTG 
CAGGAGAAGG ATGTGGATGA GCCCGAGAAA CAAGCTAAGG AGAAAGAGGA GGAGGACATG 
GAGTTCACAG TAAGATCTTA CTGTGAGACG CAGTACAACT CCGTGGCTAT GTTGGGAAAC 
GTCAGTTCAG AAGCATCGCG CAAGAAACAG GTGGAGGAGG TTCAGAACCG GCATCGGCTC 
ATTCCATTGG GTCGCAAAAT ATATGAGTTT TACAACGCCC CTATCGTCAA GTTCTGGTTT 
CACACGCTGG CGTATGTAGG ATACTTGATG CTCTTCAACT ATATTGTGCT GGTGAAGATG 
GACCTGTGGC CTTCTCCTCA GGAGTGGATT GTTATTGCCT ACATATTCAC CAATGGCATC 
GAGAAGATGA GAGAGATCCT GATGTCCGAA CCTGGGAAGC TGCTTCAGAA GGTGAAAGTG 
TGGTTACAGG AGTACTGGAA CATCACTGAC CTCATGGCCA TCCTCATCTT CTCCATCGGG 
ATGGTGCTTC GTCTGCAGGA CCCGCCGCTG ATGAGCTACG GCAGGGTCAT TTACTGCGTC 
AATATCATTT ATTGGTACAT ACGACTCCTC GACATCTTCG GAGTGAACAA ATACCTGGGT 
CCATACGTCA TGATGATCGG CAAGATGATG ATTGACATGA TGTATTTTGT GATCATCATG 
TTGGTGGTGC TGATGAGTTT CGGTGTGGCG CGTCAGGCCA TCCTTAATCC CAATGAAGAC 
CCATCCTGGA TGCTGGCACG CAATATCTTC TTCATGCCAT ACTGGATGAT CTACGGAGAG 
GTGTTTGCTG ACCAAATCGA CCCTCCTTGT GGTCAGAACA TCACCACAGA AGAAGGAGTT 
GTCATGCCTC TTCCACCCTG CAAAACTGGA GCTTGGATTG TCCCTGCCAT AATGGCCTGC 
TACCTGCTGG TGGCCAACAT CCTGCTAGTT AACTTGCTCA TTGCTGTCTT TAATAACACT 
TTCTTTGAAG TCAAGTCCAT TTCCAACCAG GTGTGGAAGT TTCAGAGGTA TCAGCTGATC 
ATGACCTTCC ATGAACGCCC GGTCCTTCCT CCTCCACTCA TCATCTTCAG CCACATAACT 
ATGGTTCTCA AACACCTGTG CTGTCGCTGG AGGAAGCACG ATGAGGATGA ACGGGACTAT 
GGGCTGAAGC TTTTCATCAC TGAAGATGAA CTGAAGAAAG TGCATGACTT TGAAGAGCAG 
TGCATGGAGG AGTATTTCAG AGAGAAAGAT GACCGCTTCA ATTCCTCCAA TGACGAAAGG 
ATACGTGTGA CATCTGAAAG GGTGGAGAAC ATGGCGATGC GATTAGAGGA GGTGAATGAG 
AGGGAACATT TTATGAAAGC GTCCTTGCAG ACTGTAGACA TCCGGCTCGC CCAGATGGAG 
GAGATGATCG GCCGCATTGC TGTGGCCCTA GAGAGAGTTG CTGGAATGGA CAGAGGCGAA 
GTTAACAAAG CCCGGTCCAG GACATCATCA GACTGCACTG ATACCAATTA CATCCTGCGC 
CAAAGCAGTT TCAACAGCCA AGAAGGCAAT TCCTACAGGC TACAGGAGTC TCTGGAGCAG 
GGGGGCGACG AGTCCATTTC TCCTACTTCC CCAACCGCCC TGGCTCCCCG TGTCCGCAGT 
CACTCCTTCT ACGTGAGCCA CTCTTCCAAA GACAGGAGTG GGGCGGACCG TGGAGAGGGC 
TTCTTTAAAG ACAAGTTGTT CAGCCTCCAC CGGGCCAACA GCTCACAGTC TGTGTCCTCA 
GGAGCAGGTC CCAAAGAGTC CAAGCCAACC CCTTTGAATA CTTTATCCGT GCAGCAGCAG 
CTCCGCCCTT CTTCCTGCAT AGACATCTAT GTTTCTGCCT CAGAGGACGT CCCGCCCACT 
GAGAGCTTCT TGGATTCGGT TCGGACAGTT CCAACTCTGG CTCGAGACTC ATCTCTTCAT 
TCGGAGATCA TGGAGGCGGT GCTCTCAGGG GGACGGGACT GTAGTGGAAG GGCAGGCGGC 
AGCGAAAGAC AGTCGGATGG TACAGTCCTG TTTGAGGACA GCGCCGCCGC TGACCTATCG 
CTCTGCTCTG CCCACCTGCT GCCGGATTCC TTGCCCCCCT GGGACCTGGA CCCCTCACCG 
CCACCATCAG CTGGTGTACT AGAGCGGTCC AAAAGTAGTC GCTTCCTGTC TACAGCAGGG 
CCTCTGTTTC TGGACGAGCC TCATTTGGTT AAGTCACACA GTCTGATGTT CACATCACGT 
GGGTACTATG GTGGCATGGG TGTGCAGGTG AAGGCGGCCG AATACACCAG CATCACAGAT 
TGCATCGACA CCCGTTGCGT CTCAACCCCT TACCCTGTGC CAGAGCGGTC AGACTCACCT 
GGAGGATCTT TCACCTTCGA TAAGCCTCAA GACCTTGGCG TCTCGCACCC TGAGAGAGAC 
GCTGAGCTCA GCCACGCTGA GTCTGACTTA GAGGAGCCAG CAGAGGGTTC GGGAGACACA 
TGTAAGGTGG GTCAGAGTAG CAGTAGTGGG GTAATAGGTG CAGACCTGGG CCTGGGCCTC 
GCACTAGGCC CGTTTTGCTC ACCCATCTCT AGACTAGAGC GAGCAAACAG CTGCTCCTCT 
TCAGAGGAGT CGCACTCCAA CATCTACTCT CGAAAGAGCT TCTCAATAAG CGAGAGAATG 
GACAAGGGCC GGGGGAGCTC CAGGAACCCT TTCCAGAAGG CCAGAACCGG GGCGAGGCTG 
GAGGGTAAGA CAGACTCACT GTCTATGAGG AAAATGGCCA AACCGTCAGC TTTCAGAAGT 
TTTGACAGCA GACACAACTA CACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299837.1
CDS	175..4161
Translation	MQLRITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQSMLDALVLDRVDFVKLLIENGVSMHRFLTLSRLEELYNTRHGPSNTLYHLVRDVKKQEYPGFSWIYLKGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDMPIRRGRQKTTRKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNQNSREFGQLAVELLDQSYKQDEQMAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGLLLPPSILSLEFKNKDEMSYMPQDQDTYLQEKDVDEPEKQAKEKEEEDMEFTVRSYCETQYNSVAMLGNVSSEASRKKQVEEVQNRHRLIPLGRKIYEFYNAPIVKFWFHTLAYVGYLMLFNYIVLVKMDLWPSPQEWIVIAYIFTNGIEKMREILMSEPGKLLQKVKVWLQEYWNITDLMAILIFSIGMVLRLQDPPLMSYGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDPPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHITMVLKHLCCRWRKHDEDERDYGLKLFITEDELKKVHDFEEQCMEEYFREKDDRFNSSNDERIRVTSERVENMAMRLEEVNEREHFMKASLQTVDIRLAQMEEMIGRIAVALERVAGMDRGEVNKARSRTSSDCTDTNYILRQSSFNSQEGNSYRLQESLEQGGDESISPTSPTALAPRVRSHSFYVSHSSKDRSGADRGEGFFKDKLFSLHRANSSQSVSSGAGPKESKPTPLNTLSVQQQLRPSSCIDIYVSASEDVPPTESFLDSVRTVPTLARDSSLHSEIMEAVLSGGRDCSGRAGGSERQSDGTVLFEDSAAADLSLCSAHLLPDSLPPWDLDPSPPPSAGVLERSKSSRFLSTAGPLFLDEPHLVKSHSLMFTSRGYYGGMGVQVKAAEYTSITDCIDTRCVSTPYPVPERSDSPGGSFTFDKPQDLGVSHPERDAELSHAESDLEEPAEGSGDTCKVGQSSSSGVIGADLGLGLALGPFCSPISRLERANSCSSSEESHSNIYSRKSFSISERMDKGRGSSRNPFQKARTGARLEGKTDSLSMRKMAKPSAFRSFDSRHNYT

Target ORF information:

RefSeq Version	XM_009301562.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301562.2

ORF Insert Sequence:

ATGCAACTCA GGATCACAGT GTTTCGGATG GGATCAGAGG GACACCAGGA CATCGATCTA 
GCCATTCTAA CAGCTCTTCT GAAAGGAGCG AATGCATCAG CTCCAGATCA GCTAAGTCTG 
GCTTTGGCCT GGAACAGAGT TGACATCGCC CGGAGCCAGA TCTTCATTTA CGGACAGCAG 
TGGCCTGTTG GCTCTCTGGA GCAGTCTATG CTGGACGCGC TGGTGCTGGA CCGAGTGGAC 
TTTGTAAAGC TGTTGATTGA GAACGGAGTG AGCATGCACC GTTTCCTGAC ACTCTCCAGA 
CTTGAAGAGC TTTATAATAC GAGGCACGGA CCATCCAACA CCTTGTACCA CCTTGTCAGA 
GATGTGAAAA AGCAAGAATA TCCAGGGTTC AGTTGGATCT ACCTCAAGGG TAACCTGCCC 
CCCGACTACC GCATTAGCCT GATTGACATC GGATTGGTTA TTGAATACCT CATGGGTGGA 
GCCTATCGCT GCAATTACAC CAGGAAGAGA TTTCGGACCC TCTATCACAA CCTCTTTGGA 
CCCAAAAGAC CAAAAGCCCT GAAGCTGCTT GGAATGGAGG ATGACATGCC CATTAGACGA 
GGGCGTCAGA AGACTACGCG GAAGCGAGAG GAGGAAGTAG ATATTGACCT GGATGACCCA 
GAAATCAATC ATTTCCCTTT CCCATTCCAT GAGCTGATGG TTTGGGCTGT GCTGATGAAA 
CGGCAGAAGA TGGCACTGTT CTTCTGGCAG CATGGAGAGG AGGCCATGGC TAAAGCGCTG 
GTGGCCTGCA AGCTCTGTAA GGCAATGGCT CACGAAGCTT CTGAGAACGA CATGGTCGAT 
GACATTTCAC AGGAGCTCAA TCAAAACTCA AGGGAGTTTG GTCAGCTGGC TGTTGAGCTG 
CTCGATCAGT CCTATAAGCA GGATGAACAG ATGGCTATGA AGCTGTTGAC CTACGAGCTG 
AAGAACTGGA GTAATGCCAC CTGTCTGCAG CTGGCAGTGG CTGCCAAGCA CCGAGACTTC 
ATCGCCCACA CCTGTAGCCA GATGCTGCTC ACAGACATGT GGATGGGACG TCTGCGCATG 
CGCAAGAACT CTGGCCTGAA GGTTATCTTG GGCCTGCTTC TCCCCCCTTC CATATTGAGT 
CTGGAGTTTA AAAATAAAGA CGAGATGTCA TACATGCCTC AGGATCAGGA CACATATCTG 
CAGGAGAAGG ATGTGGATGA GCCCGAGAAA CAAGCTAAGG AGAAAGAGGA GGAGGACATG 
GAGTTCACAG TAAGATCTTA CTGTGAGACG CAGTACAACT CCGTGGCTAT GTTGGGAAAC 
GTCAGTTCAG AAGCATCGCG CAAGAAACAG GTGGAGGAGG TTCAGAACCG GCATCGGCTC 
ATTCCATTGG GTCGCAAAAT ATATGAGTTT TACAACGCCC CTATCGTCAA GTTCTGGTTT 
CACACGCTGG CGTATGTAGG ATACTTGATG CTCTTCAACT ATATTGTGCT GGTGAAGATG 
GACCTGTGGC CTTCTCCTCA GGAGTGGATT GTTATTGCCT ACATATTCAC CAATGGCATC 
GAGAAGATGA GAGAGATCCT GATGTCCGAA CCTGGGAAGC TGCTTCAGAA GGTGAAAGTG 
TGGTTACAGG AGTACTGGAA CATCACTGAC CTCATGGCCA TCCTCATCTT CTCCATCGGG 
ATGGTGCTTC GTCTGCAGGA CCCGCCGCTG ATGAGCTACG GCAGGGTCAT TTACTGCGTC 
AATATCATTT ATTGGTACAT ACGACTCCTC GACATCTTCG GAGTGAACAA ATACCTGGGT 
CCATACGTCA TGATGATCGG CAAGATGATG ATTGACATGA TGTATTTTGT GATCATCATG 
TTGGTGGTGC TGATGAGTTT CGGTGTGGCG CGTCAGGCCA TCCTTAATCC CAATGAAGAC 
CCATCCTGGA TGCTGGCACG CAATATCTTC TTCATGCCAT ACTGGATGAT CTACGGAGAG 
GTGTTTGCTG ACCAAATCGA CCCTCCTTGT GGTCAGAACA TCACCACAGA AGAAGGAGTT 
GTCATGCCTC TTCCACCCTG CAAAACTGGA GCTTGGATTG TCCCTGCCAT AATGGCCTGC 
TACCTGCTGG TGGCCAACAT CCTGCTAGTT AACTTGCTCA TTGCTGTCTT TAATAACACT 
TTCTTTGAAG TCAAGTCCAT TTCCAACCAG GTGTGGAAGT TTCAGAGGTA TCAGCTGATC 
ATGACCTTCC ATGAACGCCC GGTCCTTCCT CCTCCACTCA TCATCTTCAG CCACATAACT 
ATGGTTCTCA AACACCTGTG CTGTCGCTGG AGGAAGCACG ATGAGGATGA ACGGGACTAT 
GGGCTGAAGC TTTTCATCAC TGAAGATGAA CTGAAGAAAG TGCATGACTT TGAAGAGCAG 
TGCATGGAGG AGTATTTCAG AGAGAAAGAT GACCGCTTCA ATTCCTCCAA TGACGAAAGG 
ATACGTGTGA CATCTGAAAG GGTGGAGAAC ATGGCGATGC GATTAGAGGA GGTGAATGAG 
AGGGAACATT TTATGAAAGC GTCCTTGCAG ACTGTAGACA TCCGGCTCGC CCAGATGGAG 
GAGATGATCG GCCGCATTGC TGTGGCCCTA GAGAGAGTTG CTGGAATGGA CAGAGGCGAA 
GTTAACAAAG CCCGGTCCAG GACATCATCA GACTGCACTG ATACCAATTA CATCCTGCGC 
CAAAGCAGTT TCAACAGCCA AGAAGGCAAT TCCTACAGGC TACAGGAGTC TCTGGAGCAG 
GGGGGCGACG AGTCCATTTC TCCTACTTCC CCAACCGCCC TGGCTCCCCG TGTCCGCAGT 
CACTCCTTCT ACGTGAGCCA CTCTTCCAAA GACAGGAGTG GGGCGGACCG TGGAGAGGGC 
TTCTTTAAAG ACAAGTTGTT CAGCCTCCAC CGGGCCAACA GCTCACAGTC TGTGTCCTCA 
GGAGCAGGTC CCAAAGAGTC CAAGCCAACC CCTTTGAATA CTTTATCCGT GCAGCAGCAG 
CTCCGCCCTT CTTCCTGCAT AGACATCTAT GTTTCTGCCT CAGAGGACGT CCCGCCCACT 
GAGAGCTTCT TGGATTCGGT TCGGACAGTT CCAACTCTGG CTCGAGACTC ATCTCTTCAT 
TCGGAGATCA TGGAGGCGGT GCTCTCAGGG GGACGGGACT GTAGTGGAAG GGCAGGCGGC 
AGCGAAAGAC AGTCGGATGG TACAGTCCTG TTTGAGGACA GCGCCGCCGC TGACCTATCG 
CTCTGCTCTG CCCACCTGCT GCCGGATTCC TTGCCCCCCT GGGACCTGGA CCCCTCACCG 
CCACCATCAG CTGGTGTACT AGAGCGGTCC AAAAGTAGTC GCTTCCTGTC TACAGCAGGG 
CCTCTGTTTC TGGACGAGCC TCATTTGGTT AAGTCACACA GTCTGATGTT CACATCACGT 
GGGTACTATG GTGGCATGGG TGTGCAGGTG AAGGCGGCCG AATACACCAG CATCACAGAT 
TGCATCGACA CCCGTTGCGT CTCAACCCCT TACCCTGTGC CAGAGCGGTC AGACTCACCT 
GGAGGATCTT TCACCTTCGA TAAGCCTCAA GACCTTGGCG TCTCGCACCC TGAGAGAGAC 
GCTGAGCTCA GCCACGCTGA GTCTGACTTA GAGGAGCCAG CAGAGGGTTC GGGAGACACA 
TGTAAGGTGG GTCAGAGTAG CAGTAGTGGG GTAATAGGTG CAGACCTGGG CCTGGGCCTC 
GCACTAGGCC CGTTTTGCTC ACCCATCTCT AGACTAGAGC GAGCAAACAG CTGCTCCTCT 
TCAGAGGAGT CGCACTCCAA CATCTACTCT CGAAAGAGCT TCTCAATAAG CGAGAGAATG 
GACAAGGGCC GGGGGAGCTC CAGGAACCCT TTCCAGAAGG CCAGAACCGG GGCGAGGCTG 
GAGGGTAAGA CAGACTCACT GTCTATGAGG AAAATGGCCA AACCGTCAGC TTTCAGAAGT 
TTTGACAGCA GACACAACTA CACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm3 ( XM_009301563.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299838.1 Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X11, mRNA.

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CloneID	ODa44723
Clone ID Related Accession (Same CDS sequence)	XM_009301563.2
Accession Version	XM_009301563.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transient receptor potential cation channel subfamily M member 3 isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301563.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAAAA GAAAATGGGA GGGGAGGAGA GAAGGCTTCA AGGTGAGCTC CACGAGGGAG 
GAGGCACAGG GGCCTGGACG CCCCTCCACC AGAGGGCGCT TCTCCGAATC TTGGAAGAGA 
CTGAGCTCCA GACAGGGGTC TACCAAGAGG TCAGGACTGG CCTCCCAGCA AGCTGCGGCA 
CAGAAATCAT GGATAGAAAG AGCGTTCAGC AAGAGAGAAT GTGTGCACAT TATTGTAAGC 
ACCAAAGACC ACCATAGGTG CTGTTGTGGC CGTCTAATTG GTCAACATGT TGGCCTGCCC 
CCCAGCATCT CATCCAATCA GAATGAGAAG TCAGAACGCG TGCCAAAGAA TGACAGCTTG 
TCAGAGAAGT GGTCCATCAG CAAGCACACC CAGCTCAGTC CCACCGACGC TTTTGGGACC 
ATCGAGTTCC AGGGAGGAGG ACACTCTAAT AAAGCAATGT ATGTACGAGT GTCTTACGAC 
ACAAAACCAG ATTTACTGCT GCATCTGATG ACAAAGGAGT GGCAACTGGA GCTTCCCAAG 
CTGCTCATCT CTGTTCACGG AGGACTTCAA AACTTTGAGT TGCAGCCCAA ACTAAAGCAA 
GTCTTCGGCA AAGGTCTTAT AAAGGCTGCA ATGACTACCG GAGCCTGGAT CTTCACTGGA 
GGAGTTAATA CTGGAGTCAT CAGACATGTT GGTGATGCTC TGAAAGATCA TGCTTCCAAA 
TCAAGAGGGA AGATCTGCAC TATCGGCATT GCCCCCTGGG GGATTGTGGA GAACCAGGAA 
GACCTGGTGG GCAAAGATGT GGTCCGGCCA TACCAGACGA TGTCTAACCC TCTTAGCAAG 
CTAACAGTAC TCAACAGCCT ACACTCACAC TTCATTTTGG CTGATAATGG CACCACCGGA 
AAGTACGGAG CAGAAGTCAA ACTGCGCAGG CAGCTGGAGA AGCACATCTC TCTGCAAAAG 
ATTAACACAC GGATTGGACA GGGTGTTCCA GTAGTGGCTC TGATAGTGGA GGGGGGTCCG 
AATGTCATCT CTATCGTCCT GGAGTACCTA AGAGACACCC CTCCGGTGCC TGTTGTGGTG 
TGTGACGGCA GCGGTCGAGC CTCTGACATC CTGGCCTTTG GGCACAAGTA CTCAGAGGAG 
GGCGGAATCA TAAACGAATC TTTGAGAGAC CAGTTATTAG TCACCATTCA GAAGACGTTC 
ACATACAGCC GGACGCAGGC CCAGCATCTC TTCATCATTC TGATGGAGTG CATGAAGAAG 
AAAGAACTGA TCACAGTGTT TCGGATGGGA TCAGAGGGAC ACCAGGACAT CGATCTAGCC 
ATTCTAACAG CTCTTCTGAA AGGAGCGAAT GCATCAGCTC CAGATCAGCT AAGTCTGGCT 
TTGGCCTGGA ACAGAGTTGA CATCGCCCGG AGCCAGATCT TCATTTACGG ACAGCAGTGG 
CCTGTTGGCT CTCTGGAGCA GTCTATGCTG GACGCGCTGG TGCTGGACCG AGTGGACTTT 
GTAAAGCTGT TGATTGAGAA CGGAGTGAGC ATGCACCGTT TCCTGACACT CTCCAGACTT 
GAAGAGCTTT ATAATACGAG GCACGGACCA TCCAACACCT TGTACCACCT TGTCAGAGAT 
GTGAAAAAGC AAGAATATCC AGGGTTCAGT TGGATCTACC TCAAGGGTAA CCTGCCCCCC 
GACTACCGCA TTAGCCTGAT TGACATCGGA TTGGTTATTG AATACCTCAT GGGTGGAGCC 
TATCGCTGCA ATTACACCAG GAAGAGATTT CGGACCCTCT ATCACAACCT CTTTGGACCC 
AAAAGACCAA AAGCCCTGAA GCTGCTTGGA ATGGAGGATG ACATGCCCAT TAGACGAGGG 
CGTCAGAAGA CTACGCGGAA GCGAGAGGAG GAAGTAGATA TTGACCTGGA TGACCCAGAA 
ATCAATCATT TCCCTTTCCC ATTCCATGAG CTGATGGTTT GGGCTGTGCT GATGAAACGG 
CAGAAGATGG CACTGTTCTT CTGGCAGCAT GGAGAGGAGG CCATGGCTAA AGCGCTGGTG 
GCCTGCAAGC TCTGTAAGGC AATGGCTCAC GAAGCTTCTG AGAACGACAT GGTCGATGAC 
ATTTCACAGG AGCTCAATCA AAACTCAAGG GAGTTTGGTC AGCTGGCTGT TGAGCTGCTC 
GATCAGTCCT ATAAGCAGGA TGAACAGATG GCTATGAAGC TGTTGACCTA CGAGCTGAAG 
AACTGGAGTA ATGCCACCTG TCTGCAGCTG GCAGTGGCTG CCAAGCACCG AGACTTCATC 
GCCCACACCT GTAGCCAGAT GCTGCTCACA GACATGTGGA TGGGACGTCT GCGCATGCGC 
AAGAACTCTG GCCTGAAGGT TATCTTGGGC CTGCTTCTCC CCCCTTCCAT ATTGAGTCTG 
GAGTTTAAAA ATAAAGACGA GATGTCATAC ATGCCTCAGG ATCAGGACAC ATATCTGCAG 
GAGAAGGATG TGGATGAGCC CGAGAAACAA GCTAAGGAGA AAGAGGAGGA GGACATGGAG 
TTCACAGTAA GATCTTACTG TGAGACGCAG TACAACTCCG TGGCTATGTT GGGAAACGTC 
AGTTCAGAAG CATCGCGCAA GAAACAGGTG GAGGAGGTTC AGAACCGGCA TCGGCTCATT 
CCATTGGGTC GCAAAATATA TGAGTTTTAC AACGCCCCTA TCGTCAAGTT CTGGTTTCAC 
ACGCTGGCGT ATGTAGGATA CTTGATGCTC TTCAACTATA TTGTGCTGGT GAAGATGGAC 
CTGTGGCCTT CTCCTCAGGA GTGGATTGTT ATTGCCTACA TATTCACCAA TGGCATCGAG 
AAGATGAGAG AGATCCTGAT GTCCGAACCT GGGAAGCTGC TTCAGAAGGT GAAAGTGTGG 
TTACAGGAGT ACTGGAACAT CACTGACCTC ATGGCCATCC TCATCTTCTC CATCGGGATG 
GTGCTTCGTC TGCAGGACCC GCCGCTGATG AGCTACGGCA GGGTCATTTA CTGCGTCAAT 
ATCATTTATT GGTACATACG ACTCCTCGAC ATCTTCGGAG TGAACAAATA CCTGGGTCCA 
TACGTCATGA TGATCGGCAA GATGATGATT GACATGATGT ATTTTGTGAT CATCATGTTG 
GTGGTGCTGA TGAGTTTCGG TGTGGCGCGT CAGGCCATCC TTAATCCCAA TGAAGACCCA 
TCCTGGATGC TGGCACGCAA TATCTTCTTC ATGCCATACT GGATGATCTA CGGAGAGGTG 
TTTGCTGACC AAATCGACCA TGTGAATAGT AGGAAGCTAC ATCCCAAAGT GAATGACAAA 
CTATGGCTGG TCGAAAATTA CATGCGCACT CAAGGAACTG GGCGTTACAG TGGTCCATCC 
CATATAGGCA ATATCCAATA TTTAAACTCT GCTAGATTTA TCCAGAGCCA TTGTAATTTC 
ACAGTCACGT CATCACCTCA TCTCTGCCCC CTGATGGTCA TTGAGTGTAT GGCACCCCTG 
CACGCCACCG CAAGGCTAAT GGCCGGGTTT CCGTGGTGGG GCAAATGCTT AAGGTGTTAT 
GACAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299838.1
CDS	338..3886
Translation	MTKRKWEGRREGFKVSSTREEAQGPGRPSTRGRFSESWKRLSSRQGSTKRSGLASQQAAAQKSWIERAFSKRECVHIIVSTKDHHRCCCGRLIGQHVGLPPSISSNQNEKSERVPKNDSLSEKWSISKHTQLSPTDAFGTIEFQGGGHSNKAMYVRVSYDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLVGKDVVRPYQTMSNPLSKLTVLNSLHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGIINESLRDQLLVTIQKTFTYSRTQAQHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQSMLDALVLDRVDFVKLLIENGVSMHRFLTLSRLEELYNTRHGPSNTLYHLVRDVKKQEYPGFSWIYLKGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDMPIRRGRQKTTRKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNQNSREFGQLAVELLDQSYKQDEQMAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGLLLPPSILSLEFKNKDEMSYMPQDQDTYLQEKDVDEPEKQAKEKEEEDMEFTVRSYCETQYNSVAMLGNVSSEASRKKQVEEVQNRHRLIPLGRKIYEFYNAPIVKFWFHTLAYVGYLMLFNYIVLVKMDLWPSPQEWIVIAYIFTNGIEKMREILMSEPGKLLQKVKVWLQEYWNITDLMAILIFSIGMVLRLQDPPLMSYGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDHVNSRKLHPKVNDKLWLVENYMRTQGTGRYSGPSHIGNIQYLNSARFIQSHCNFTVTSSPHLCPLMVIECMAPLHATARLMAGFPWWGKCLRCYDN

Target ORF information:

RefSeq Version	XM_009301563.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301563.2

ORF Insert Sequence:

ATGACAAAAA GAAAATGGGA GGGGAGGAGA GAAGGCTTCA AGGTGAGCTC CACGAGGGAG 
GAGGCACAGG GGCCTGGACG CCCCTCCACC AGAGGGCGCT TCTCCGAATC TTGGAAGAGA 
CTGAGCTCCA GACAGGGGTC TACCAAGAGG TCAGGACTGG CCTCCCAGCA AGCTGCGGCA 
CAGAAATCAT GGATAGAAAG AGCGTTCAGC AAGAGAGAAT GTGTGCACAT TATTGTAAGC 
ACCAAAGACC ACCATAGGTG CTGTTGTGGC CGTCTAATTG GTCAACATGT TGGCCTGCCC 
CCCAGCATCT CATCCAATCA GAATGAGAAG TCAGAACGCG TGCCAAAGAA TGACAGCTTG 
TCAGAGAAGT GGTCCATCAG CAAGCACACC CAGCTCAGTC CCACCGACGC TTTTGGGACC 
ATCGAGTTCC AGGGAGGAGG ACACTCTAAT AAAGCAATGT ATGTACGAGT GTCTTACGAC 
ACAAAACCAG ATTTACTGCT GCATCTGATG ACAAAGGAGT GGCAACTGGA GCTTCCCAAG 
CTGCTCATCT CTGTTCACGG AGGACTTCAA AACTTTGAGT TGCAGCCCAA ACTAAAGCAA 
GTCTTCGGCA AAGGTCTTAT AAAGGCTGCA ATGACTACCG GAGCCTGGAT CTTCACTGGA 
GGAGTTAATA CTGGAGTCAT CAGACATGTT GGTGATGCTC TGAAAGATCA TGCTTCCAAA 
TCAAGAGGGA AGATCTGCAC TATCGGCATT GCCCCCTGGG GGATTGTGGA GAACCAGGAA 
GACCTGGTGG GCAAAGATGT GGTCCGGCCA TACCAGACGA TGTCTAACCC TCTTAGCAAG 
CTAACAGTAC TCAACAGCCT ACACTCACAC TTCATTTTGG CTGATAATGG CACCACCGGA 
AAGTACGGAG CAGAAGTCAA ACTGCGCAGG CAGCTGGAGA AGCACATCTC TCTGCAAAAG 
ATTAACACAC GGATTGGACA GGGTGTTCCA GTAGTGGCTC TGATAGTGGA GGGGGGTCCG 
AATGTCATCT CTATCGTCCT GGAGTACCTA AGAGACACCC CTCCGGTGCC TGTTGTGGTG 
TGTGACGGCA GCGGTCGAGC CTCTGACATC CTGGCCTTTG GGCACAAGTA CTCAGAGGAG 
GGCGGAATCA TAAACGAATC TTTGAGAGAC CAGTTATTAG TCACCATTCA GAAGACGTTC 
ACATACAGCC GGACGCAGGC CCAGCATCTC TTCATCATTC TGATGGAGTG CATGAAGAAG 
AAAGAACTGA TCACAGTGTT TCGGATGGGA TCAGAGGGAC ACCAGGACAT CGATCTAGCC 
ATTCTAACAG CTCTTCTGAA AGGAGCGAAT GCATCAGCTC CAGATCAGCT AAGTCTGGCT 
TTGGCCTGGA ACAGAGTTGA CATCGCCCGG AGCCAGATCT TCATTTACGG ACAGCAGTGG 
CCTGTTGGCT CTCTGGAGCA GTCTATGCTG GACGCGCTGG TGCTGGACCG AGTGGACTTT 
GTAAAGCTGT TGATTGAGAA CGGAGTGAGC ATGCACCGTT TCCTGACACT CTCCAGACTT 
GAAGAGCTTT ATAATACGAG GCACGGACCA TCCAACACCT TGTACCACCT TGTCAGAGAT 
GTGAAAAAGC AAGAATATCC AGGGTTCAGT TGGATCTACC TCAAGGGTAA CCTGCCCCCC 
GACTACCGCA TTAGCCTGAT TGACATCGGA TTGGTTATTG AATACCTCAT GGGTGGAGCC 
TATCGCTGCA ATTACACCAG GAAGAGATTT CGGACCCTCT ATCACAACCT CTTTGGACCC 
AAAAGACCAA AAGCCCTGAA GCTGCTTGGA ATGGAGGATG ACATGCCCAT TAGACGAGGG 
CGTCAGAAGA CTACGCGGAA GCGAGAGGAG GAAGTAGATA TTGACCTGGA TGACCCAGAA 
ATCAATCATT TCCCTTTCCC ATTCCATGAG CTGATGGTTT GGGCTGTGCT GATGAAACGG 
CAGAAGATGG CACTGTTCTT CTGGCAGCAT GGAGAGGAGG CCATGGCTAA AGCGCTGGTG 
GCCTGCAAGC TCTGTAAGGC AATGGCTCAC GAAGCTTCTG AGAACGACAT GGTCGATGAC 
ATTTCACAGG AGCTCAATCA AAACTCAAGG GAGTTTGGTC AGCTGGCTGT TGAGCTGCTC 
GATCAGTCCT ATAAGCAGGA TGAACAGATG GCTATGAAGC TGTTGACCTA CGAGCTGAAG 
AACTGGAGTA ATGCCACCTG TCTGCAGCTG GCAGTGGCTG CCAAGCACCG AGACTTCATC 
GCCCACACCT GTAGCCAGAT GCTGCTCACA GACATGTGGA TGGGACGTCT GCGCATGCGC 
AAGAACTCTG GCCTGAAGGT TATCTTGGGC CTGCTTCTCC CCCCTTCCAT ATTGAGTCTG 
GAGTTTAAAA ATAAAGACGA GATGTCATAC ATGCCTCAGG ATCAGGACAC ATATCTGCAG 
GAGAAGGATG TGGATGAGCC CGAGAAACAA GCTAAGGAGA AAGAGGAGGA GGACATGGAG 
TTCACAGTAA GATCTTACTG TGAGACGCAG TACAACTCCG TGGCTATGTT GGGAAACGTC 
AGTTCAGAAG CATCGCGCAA GAAACAGGTG GAGGAGGTTC AGAACCGGCA TCGGCTCATT 
CCATTGGGTC GCAAAATATA TGAGTTTTAC AACGCCCCTA TCGTCAAGTT CTGGTTTCAC 
ACGCTGGCGT ATGTAGGATA CTTGATGCTC TTCAACTATA TTGTGCTGGT GAAGATGGAC 
CTGTGGCCTT CTCCTCAGGA GTGGATTGTT ATTGCCTACA TATTCACCAA TGGCATCGAG 
AAGATGAGAG AGATCCTGAT GTCCGAACCT GGGAAGCTGC TTCAGAAGGT GAAAGTGTGG 
TTACAGGAGT ACTGGAACAT CACTGACCTC ATGGCCATCC TCATCTTCTC CATCGGGATG 
GTGCTTCGTC TGCAGGACCC GCCGCTGATG AGCTACGGCA GGGTCATTTA CTGCGTCAAT 
ATCATTTATT GGTACATACG ACTCCTCGAC ATCTTCGGAG TGAACAAATA CCTGGGTCCA 
TACGTCATGA TGATCGGCAA GATGATGATT GACATGATGT ATTTTGTGAT CATCATGTTG 
GTGGTGCTGA TGAGTTTCGG TGTGGCGCGT CAGGCCATCC TTAATCCCAA TGAAGACCCA 
TCCTGGATGC TGGCACGCAA TATCTTCTTC ATGCCATACT GGATGATCTA CGGAGAGGTG 
TTTGCTGACC AAATCGACCA TGTGAATAGT AGGAAGCTAC ATCCCAAAGT GAATGACAAA 
CTATGGCTGG TCGAAAATTA CATGCGCACT CAAGGAACTG GGCGTTACAG TGGTCCATCC 
CATATAGGCA ATATCCAATA TTTAAACTCT GCTAGATTTA TCCAGAGCCA TTGTAATTTC 
ACAGTCACGT CATCACCTCA TCTCTGCCCC CTGATGGTCA TTGAGTGTAT GGCACCCCTG 
CACGCCACCG CAAGGCTAAT GGCCGGGTTT CCGTGGTGGG GCAAATGCTT AAGGTGTTAT 
GACAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm3 ( XM_021476467.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332142.1 Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa142786
Clone ID Related Accession (Same CDS sequence)	XM_021476467.1
Accession Version	XM_021476467.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily M member 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAAAA GAAAATGGGA GGGGAGGAGA GAAGGCTTCA AGGTGAGCTC CACGAGGGAG 
GAGGCACAGG GGCCTGGACG CCCCTCCACC AGAGGGCGCT TCTCCGAATC TTGGAAGAGA 
CTGAGCTCCA GACAGGGGTC TACCAAGAGG TCAGGACTGG CCTCCCAGCA AGCTGCGGCA 
CAGAAATCAT GGATAGAAAG AGCGTTCAGC AAGAGAGAAT GTGTGCACAT TATTGTAAGC 
ACCAAAGACC ACCATAGGTG CTGTTGTGGC CGTCTAATTG GTCAACATGT TGGCCTGCCC 
CCCAGCATCT CATCCAATCA GAATGAGAAG TCAGAACGCG TGCCAAAGAA TGACAGCTTG 
TCAGAGAAGT GGTCCATCAG CAAGCACACC CAGCTCAGTC CCACCGACGC TTTTGGGACC 
ATCGAGTTCC AGGGAGGAGG ACACTCTAAT AAAGCAATGT ATGTACGAGT GTCTTACGAC 
ACAAAACCAG ATTTACTGCT GCATCTGATG ACAAAGGAGT GGCAACTGGA GCTTCCCAAG 
CTGCTCATCT CTGTTCACGG AGGACTTCAA AACTTTGAGT TGCAGCCCAA ACTAAAGCAA 
GTCTTCGGCA AAGGTCTTAT AAAGGCTGCA ATGACTACCG GAGCCTGGAT CTTCACTGGA 
GGAGTTAATA CTGGAGTCAT CAGACATGTT GGTGATGCTC TGAAAGATCA TGCTTCCAAA 
TCAAGAGGGA AGATCTGCAC TATCGGCATT GCCCCCTGGG GGATTGTGGA GAACCAGGAA 
GACCTGGTGG GCAAAGATGT GGTCCGGCCA TACCAGACGA TGTCTAACCC TCTTAGCAAG 
CTAACAGTAC TCAACAGCCT ACACTCACAC TTCATTTTGG CTGATAATGG CACCACCGGA 
AAGTACGGAG CAGAAGTCAA ACTGCGCAGG CAGCTGGAGA AGCACATCTC TCTGCAAAAG 
ATTAACACAC GGATTGGACA GGGTGTTCCA GTAGTGGCTC TGATAGTGGA GGGGGGTCCG 
AATGTCATCT CTATCGTCCT GGAGTACCTA AGAGACACCC CTCCGGTGCC TGTTGTGGTG 
TGTGACGGCA GCGGTCGAGC CTCTGACATC CTGGCCTTTG GGCACAAGTA CTCAGAGGAG 
GGCGGAATCA TAAACGAATC TTTGAGAGAC CAGTTATTAG TCACCATTCA GAAGACGTTC 
ACATACAGCC GGACGCAGGC CCAGCATCTC TTCATCATTC TGATGGAGTG CATGAAGAAG 
AAAGAACTGA TCACAGTGTT TCGGATGGGA TCAGAGGGAC ACCAGGACAT CGATCTAGCC 
ATTCTAACAG CTCTTCTGAA AGGAGCGAAT GCATCAGCTC CAGATCAGCT AAGTCTGGCT 
TTGGCCTGGA ACAGAGTTGA CATCGCCCGG AGCCAGATCT TCATTTACGG ACAGCAGTGG 
CCTGTTGGCT CTCTGGAGCA GTCTATGCTG GACGCGCTGG TGCTGGACCG AGTGGACTTT 
GTAAAGCTGT TGATTGAGAA CGGAGTGAGC ATGCACCGTT TCCTGACACT CTCCAGACTT 
GAAGAGCTTT ATAATACGAG GCACGGACCA TCCAACACCT TGTACCACCT TGTCAGAGAT 
GTGAAAAAGC AAGAATATCC AGGGTTCAGT TGGATCTACC TCAAGGGTAA CCTGCCCCCC 
GACTACCGCA TTAGCCTGAT TGACATCGGA TTGGTTATTG AATACCTCAT GGGTGGAGCC 
TATCGCTGCA ATTACACCAG GAAGAGATTT CGGACCCTCT ATCACAACCT CTTTGGACCC 
AAAAGACCAA AAGCCCTGAA GCTGCTTGGA ATGGAGGATG ACATGCCCAT TAGACGAGGG 
CGTCAGAAGA CTACGCGGAA GCGAGAGGAG GAAGTAGATA TTGACCTGGA TGACCCAGAA 
ATCAATCATT TCCCTTTCCC ATTCCATGAG CTGATGGTTT GGGCTGTGCT GATGAAACGG 
CAGAAGATGG CACTGTTCTT CTGGCAGCAT GGAGAGGAGG CCATGGCTAA AGCGCTGGTG 
GCCTGCAAGC TCTGTAAGGC AATGGCTCAC GAAGCTTCTG AGAACGACAT GGTCGATGAC 
ATTTCACAGG AGCTCAATCA AAACTCAAGG GAGTTTGGTC AGCTGGCTGT TGAGCTGCTC 
GATCAGTCCT ATAAGCAGGA TGAACAGATG GCTATGAAGC TGTTGACCTA CGAGCTGAAG 
AACTGGAGTA ATGCCACCTG TCTGCAGCTG GCAGTGGCTG CCAAGCACCG AGACTTCATC 
GCCCACACCT GTAGCCAGAT GCTGCTCACA GACATGTGGA TGGGACGTCT GCGCATGCGC 
AAGAACTCTG GCCTGAAGGT TATCTTGGGC CTGCTTCTCC CCCCTTCCAT ATTGAGTCTG 
GAGTTTAAAA ATAAAGACGA GATGTCATAC ATGCCTCAGG ATCAGGACAC ATATCTGCAG 
GAGAAGGATG TGGATGAGCC CGAGAAACAA GCTAAGGAGA AAGAGGAGGA GGACATGGAG 
TTCACAGTAA GATCTTACTG TGAGACGCAG TACAACTCCG TGGCTATGTT GGGAAACGTC 
AGTTCAGAAG CATCGCGCAA GAAACAGGTG GAGGAGGTTC AGAACCGGCA TCGGCTCATT 
CCATTGGGTC GCAAAATATA TGAGTTTTAC AACGCCCCTA TCGTCAAGTT CTGGTTTCAC 
ACGCTGGCGT ATGTAGGATA CTTGATGCTC TTCAACTATA TTGTGCTGGT GAAGATGGAC 
CTGTGGCCTT CTCCTCAGGA GTGGATTGTT ATTGCCTACA TATTCACCAA TGGCATCGAG 
AAGATGAGAG AGATCCTGAT GTCCGAACCT GGGAAGCTGC TTCAGAAGGT GAAAGTGTGG 
TTACAGGAGT ACTGGAACAT CACTGACCTC ATGGCCATCC TCATCTTCTC CATCGGGATG 
GTGCTTCGTC TGCAGGACCC GCCGCTGATG AGCTACGGCA GGGTCATTTA CTGCGTCAAT 
ATCATTTATT GGTACATACG ACTCCTCGAC ATCTTCGGAG TGAACAAATA CCTGGGTCCA 
TACGTCATGA TGATCGGCAA GATGATGATT GACATGATGT ATTTTGTGAT CATCATGTTG 
GTGGTGCTGA TGAGTTTCGG TGTGGCGCGT CAGGCCATCC TTAATCCCAA TGAAGACCCA 
TCCTGGATGC TGGCACGCAA TATCTTCTTC ATGCCATACT GGATGATCTA CGGAGAGGTG 
TTTGCTGACC AAATCGACCA TGTGAATAGT AGGAAGCTAC ATCCCAAAGT GAATGACAAA 
CTATGGCTGG TCGAAAATTA CATGCGCACT CAAGGAACTG GGCGTTACAG TGGTCCATCC 
CATATAGCTC CTTGTGGTCA GAACATCACC ACAGAAGAAG GAGTTGTCAT GCCTCTTCCA 
CCCTGCAAAA CTGGAGCTTG GATTGTCCCT GCCATAATGG CCTGCTACCT GCTGGTGGCC 
AACATCCTGC TAGTTAACTT GCTCATTGCT GTCTTTAATA ACACTTTCTT TGAAGTCAAG 
TCCATTTCCA ACCAGGTGTG GAAGTTTCAG AGGTATCAGC TGATCATGAC CTTCCATGAA 
CGCCCGGTCC TTCCTCCTCC ACTCATCATC TTCAGCCACA TAACTATGGT TCTCAAACAC 
CTGTGCTGTC GCTGGAGGAA GCACGATGAG GATGAACGGG ACTATGGGCT GAAGCTTTTC 
ATCACTGAAG ATGAACTGAA GAAAGTGCAT GACTTTGAAG AGCAGTGCAT GGAGGAGTAT 
TTCAGAGAGA AAGATGACCG CTTCAATTCC TCCAATGACG AAAGGATACG TGTGACATCT 
GAAAGGGTGG AGAACATGGC GATGCGATTA GAGGAGGTGA ATGAGAGGGA ACATTTTATG 
AAAGCGTCCT TGCAGACTGT AGACATCCGG CTCGCCCAGA TGGAGGAGAT GATCGGCCGC 
ATTGCTGTGG CCCTAGAGAG AGTTGCTGGA ATGGACAGAG GCGAAGTTAA CAAAGCCCGG 
TCCAGGACAT CATCAGACTG CACTGATACC AATTACATCC TGCGCCAAAG CAGTTTCAAC 
AGCCAAGAAG GCAATTCCTA CAGGCTACAG GAGTCTCTGG AGCAGGGGGG CGACGAGTCC 
ATTTCTCCTA CTTCCCCAAC CGCCCTGGCT CCCCGTGTCC GCAGTCACTC CTTCTACGTG 
AGCCACTCTT CCAAAGACAG GAGTGGGGCG GACCGTGGAG AGGGCTTCTT TAAAGACAAG 
TTGTTCAGCC TCCACCGGGC CAACAGCTCA CAGTCTGTGT CCTCAGGAGC AGGTCCCAAA 
GAGTCCAAGC CAACCCCTTT GAATACTTTA TCCGTGCAGC AGCAGCTCCG CCCTTCTTCC 
TGCATAGACA TCTATGTTTC TGCCTCAGAG GACGTCCCGC CCACTGAGAG CTTCTTGGAT 
TCGGTTCGGA CAGTTCCAAC TCTGGCTCGA GACTCATCTC TTCATTCGGA GATCATGGAG 
GCGGTGCTCT CAGGGGGACG GGACTGTAGT GGAAGGGCAG GCGGCAGCGA AAGACAGTCG 
GATGGTACAG TCCTGTTTGA GGACAGCGCC GCCGCTGACC TATCGCTCTG CTCTGCCCAC 
CTGCTGCCGG ATTCCTTGCC CCCCTGGGAC CTGGACCCCT CACCGCCACC ATCAGCTGGT 
GTACTAGAGC GGTCCAAAAG TAGTCGCTTC CTGTCTACAG CAGGGCCTCT GTTTCTGGAC 
GAGCCTCATT TGGTTAAGTC ACACAGTCTG ATGTTCACAT CACGTGGGTA CTATGGTGGC 
ATGGGTGTGC AGGTGAAGGC GGCCGAATAC ACCAGCATCA CAGATTGCAT CGACACCCGT 
TGCGTCTCAA CCCCTTACCC TGTGCCAGAG CGGTCAGACT CACCTGGAGG ATCTTTCACC 
TTCGATAAGC CTCAAGACCT TGGCGTCTCG CACCCTGAGA GAGACGCTGA GCTCAGCCAC 
GCTGAGTCTG ACTTAGAGGA GCCAGCAGAG GGTTCGGGAG ACACATGTAA GGTGGGTCAG 
AGTAGCAGTA GTGGGGTAAT AGGTGCAGAC CTGGGCCTGG GCCTCGCACT AGGCCCGTTT 
TGCTCACCCA TCTCTAGACT AGAGCGAGCA AACAGCTGCT CCTCTTCAGA GGAGTCGCAC 
TCCAACATCT ACTCTCGAAA GAGCTTCTCA ATAAGCGAGA GAATGGACAA GGGCCGGGGG 
AGCTCCAGGA ACCCTTTCCA GAAGGCCAGA ACCGGGGCGA GGCTGGAGGG TAAGACAGAC 
TCACTGTCTA TGAGGAAAAT GGCCAAACCG TCAGCTTTCA GAAGTTTTGA CAGCAGACAC 
AACTACACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332142.1
CDS	378..5729
Translation	MTKRKWEGRREGFKVSSTREEAQGPGRPSTRGRFSESWKRLSSRQGSTKRSGLASQQAAAQKSWIERAFSKRECVHIIVSTKDHHRCCCGRLIGQHVGLPPSISSNQNEKSERVPKNDSLSEKWSISKHTQLSPTDAFGTIEFQGGGHSNKAMYVRVSYDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLVGKDVVRPYQTMSNPLSKLTVLNSLHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGIINESLRDQLLVTIQKTFTYSRTQAQHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQSMLDALVLDRVDFVKLLIENGVSMHRFLTLSRLEELYNTRHGPSNTLYHLVRDVKKQEYPGFSWIYLKGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDMPIRRGRQKTTRKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNQNSREFGQLAVELLDQSYKQDEQMAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGLLLPPSILSLEFKNKDEMSYMPQDQDTYLQEKDVDEPEKQAKEKEEEDMEFTVRSYCETQYNSVAMLGNVSSEASRKKQVEEVQNRHRLIPLGRKIYEFYNAPIVKFWFHTLAYVGYLMLFNYIVLVKMDLWPSPQEWIVIAYIFTNGIEKMREILMSEPGKLLQKVKVWLQEYWNITDLMAILIFSIGMVLRLQDPPLMSYGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDHVNSRKLHPKVNDKLWLVENYMRTQGTGRYSGPSHIAPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHITMVLKHLCCRWRKHDEDERDYGLKLFITEDELKKVHDFEEQCMEEYFREKDDRFNSSNDERIRVTSERVENMAMRLEEVNEREHFMKASLQTVDIRLAQMEEMIGRIAVALERVAGMDRGEVNKARSRTSSDCTDTNYILRQSSFNSQEGNSYRLQESLEQGGDESISPTSPTALAPRVRSHSFYVSHSSKDRSGADRGEGFFKDKLFSLHRANSSQSVSSGAGPKESKPTPLNTLSVQQQLRPSSCIDIYVSASEDVPPTESFLDSVRTVPTLARDSSLHSEIMEAVLSGGRDCSGRAGGSERQSDGTVLFEDSAAADLSLCSAHLLPDSLPPWDLDPSPPPSAGVLERSKSSRFLSTAGPLFLDEPHLVKSHSLMFTSRGYYGGMGVQVKAAEYTSITDCIDTRCVSTPYPVPERSDSPGGSFTFDKPQDLGVSHPERDAELSHAESDLEEPAEGSGDTCKVGQSSSSGVIGADLGLGLALGPFCSPISRLERANSCSSSEESHSNIYSRKSFSISERMDKGRGSSRNPFQKARTGARLEGKTDSLSMRKMAKPSAFRSFDSRHNYT

Target ORF information:

RefSeq Version	XM_021476467.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476467.1

ORF Insert Sequence:

ATGACAAAAA GAAAATGGGA GGGGAGGAGA GAAGGCTTCA AGGTGAGCTC CACGAGGGAG 
GAGGCACAGG GGCCTGGACG CCCCTCCACC AGAGGGCGCT TCTCCGAATC TTGGAAGAGA 
CTGAGCTCCA GACAGGGGTC TACCAAGAGG TCAGGACTGG CCTCCCAGCA AGCTGCGGCA 
CAGAAATCAT GGATAGAAAG AGCGTTCAGC AAGAGAGAAT GTGTGCACAT TATTGTAAGC 
ACCAAAGACC ACCATAGGTG CTGTTGTGGC CGTCTAATTG GTCAACATGT TGGCCTGCCC 
CCCAGCATCT CATCCAATCA GAATGAGAAG TCAGAACGCG TGCCAAAGAA TGACAGCTTG 
TCAGAGAAGT GGTCCATCAG CAAGCACACC CAGCTCAGTC CCACCGACGC TTTTGGGACC 
ATCGAGTTCC AGGGAGGAGG ACACTCTAAT AAAGCAATGT ATGTACGAGT GTCTTACGAC 
ACAAAACCAG ATTTACTGCT GCATCTGATG ACAAAGGAGT GGCAACTGGA GCTTCCCAAG 
CTGCTCATCT CTGTTCACGG AGGACTTCAA AACTTTGAGT TGCAGCCCAA ACTAAAGCAA 
GTCTTCGGCA AAGGTCTTAT AAAGGCTGCA ATGACTACCG GAGCCTGGAT CTTCACTGGA 
GGAGTTAATA CTGGAGTCAT CAGACATGTT GGTGATGCTC TGAAAGATCA TGCTTCCAAA 
TCAAGAGGGA AGATCTGCAC TATCGGCATT GCCCCCTGGG GGATTGTGGA GAACCAGGAA 
GACCTGGTGG GCAAAGATGT GGTCCGGCCA TACCAGACGA TGTCTAACCC TCTTAGCAAG 
CTAACAGTAC TCAACAGCCT ACACTCACAC TTCATTTTGG CTGATAATGG CACCACCGGA 
AAGTACGGAG CAGAAGTCAA ACTGCGCAGG CAGCTGGAGA AGCACATCTC TCTGCAAAAG 
ATTAACACAC GGATTGGACA GGGTGTTCCA GTAGTGGCTC TGATAGTGGA GGGGGGTCCG 
AATGTCATCT CTATCGTCCT GGAGTACCTA AGAGACACCC CTCCGGTGCC TGTTGTGGTG 
TGTGACGGCA GCGGTCGAGC CTCTGACATC CTGGCCTTTG GGCACAAGTA CTCAGAGGAG 
GGCGGAATCA TAAACGAATC TTTGAGAGAC CAGTTATTAG TCACCATTCA GAAGACGTTC 
ACATACAGCC GGACGCAGGC CCAGCATCTC TTCATCATTC TGATGGAGTG CATGAAGAAG 
AAAGAACTGA TCACAGTGTT TCGGATGGGA TCAGAGGGAC ACCAGGACAT CGATCTAGCC 
ATTCTAACAG CTCTTCTGAA AGGAGCGAAT GCATCAGCTC CAGATCAGCT AAGTCTGGCT 
TTGGCCTGGA ACAGAGTTGA CATCGCCCGG AGCCAGATCT TCATTTACGG ACAGCAGTGG 
CCTGTTGGCT CTCTGGAGCA GTCTATGCTG GACGCGCTGG TGCTGGACCG AGTGGACTTT 
GTAAAGCTGT TGATTGAGAA CGGAGTGAGC ATGCACCGTT TCCTGACACT CTCCAGACTT 
GAAGAGCTTT ATAATACGAG GCACGGACCA TCCAACACCT TGTACCACCT TGTCAGAGAT 
GTGAAAAAGC AAGAATATCC AGGGTTCAGT TGGATCTACC TCAAGGGTAA CCTGCCCCCC 
GACTACCGCA TTAGCCTGAT TGACATCGGA TTGGTTATTG AATACCTCAT GGGTGGAGCC 
TATCGCTGCA ATTACACCAG GAAGAGATTT CGGACCCTCT ATCACAACCT CTTTGGACCC 
AAAAGACCAA AAGCCCTGAA GCTGCTTGGA ATGGAGGATG ACATGCCCAT TAGACGAGGG 
CGTCAGAAGA CTACGCGGAA GCGAGAGGAG GAAGTAGATA TTGACCTGGA TGACCCAGAA 
ATCAATCATT TCCCTTTCCC ATTCCATGAG CTGATGGTTT GGGCTGTGCT GATGAAACGG 
CAGAAGATGG CACTGTTCTT CTGGCAGCAT GGAGAGGAGG CCATGGCTAA AGCGCTGGTG 
GCCTGCAAGC TCTGTAAGGC AATGGCTCAC GAAGCTTCTG AGAACGACAT GGTCGATGAC 
ATTTCACAGG AGCTCAATCA AAACTCAAGG GAGTTTGGTC AGCTGGCTGT TGAGCTGCTC 
GATCAGTCCT ATAAGCAGGA TGAACAGATG GCTATGAAGC TGTTGACCTA CGAGCTGAAG 
AACTGGAGTA ATGCCACCTG TCTGCAGCTG GCAGTGGCTG CCAAGCACCG AGACTTCATC 
GCCCACACCT GTAGCCAGAT GCTGCTCACA GACATGTGGA TGGGACGTCT GCGCATGCGC 
AAGAACTCTG GCCTGAAGGT TATCTTGGGC CTGCTTCTCC CCCCTTCCAT ATTGAGTCTG 
GAGTTTAAAA ATAAAGACGA GATGTCATAC ATGCCTCAGG ATCAGGACAC ATATCTGCAG 
GAGAAGGATG TGGATGAGCC CGAGAAACAA GCTAAGGAGA AAGAGGAGGA GGACATGGAG 
TTCACAGTAA GATCTTACTG TGAGACGCAG TACAACTCCG TGGCTATGTT GGGAAACGTC 
AGTTCAGAAG CATCGCGCAA GAAACAGGTG GAGGAGGTTC AGAACCGGCA TCGGCTCATT 
CCATTGGGTC GCAAAATATA TGAGTTTTAC AACGCCCCTA TCGTCAAGTT CTGGTTTCAC 
ACGCTGGCGT ATGTAGGATA CTTGATGCTC TTCAACTATA TTGTGCTGGT GAAGATGGAC 
CTGTGGCCTT CTCCTCAGGA GTGGATTGTT ATTGCCTACA TATTCACCAA TGGCATCGAG 
AAGATGAGAG AGATCCTGAT GTCCGAACCT GGGAAGCTGC TTCAGAAGGT GAAAGTGTGG 
TTACAGGAGT ACTGGAACAT CACTGACCTC ATGGCCATCC TCATCTTCTC CATCGGGATG 
GTGCTTCGTC TGCAGGACCC GCCGCTGATG AGCTACGGCA GGGTCATTTA CTGCGTCAAT 
ATCATTTATT GGTACATACG ACTCCTCGAC ATCTTCGGAG TGAACAAATA CCTGGGTCCA 
TACGTCATGA TGATCGGCAA GATGATGATT GACATGATGT ATTTTGTGAT CATCATGTTG 
GTGGTGCTGA TGAGTTTCGG TGTGGCGCGT CAGGCCATCC TTAATCCCAA TGAAGACCCA 
TCCTGGATGC TGGCACGCAA TATCTTCTTC ATGCCATACT GGATGATCTA CGGAGAGGTG 
TTTGCTGACC AAATCGACCA TGTGAATAGT AGGAAGCTAC ATCCCAAAGT GAATGACAAA 
CTATGGCTGG TCGAAAATTA CATGCGCACT CAAGGAACTG GGCGTTACAG TGGTCCATCC 
CATATAGCTC CTTGTGGTCA GAACATCACC ACAGAAGAAG GAGTTGTCAT GCCTCTTCCA 
CCCTGCAAAA CTGGAGCTTG GATTGTCCCT GCCATAATGG CCTGCTACCT GCTGGTGGCC 
AACATCCTGC TAGTTAACTT GCTCATTGCT GTCTTTAATA ACACTTTCTT TGAAGTCAAG 
TCCATTTCCA ACCAGGTGTG GAAGTTTCAG AGGTATCAGC TGATCATGAC CTTCCATGAA 
CGCCCGGTCC TTCCTCCTCC ACTCATCATC TTCAGCCACA TAACTATGGT TCTCAAACAC 
CTGTGCTGTC GCTGGAGGAA GCACGATGAG GATGAACGGG ACTATGGGCT GAAGCTTTTC 
ATCACTGAAG ATGAACTGAA GAAAGTGCAT GACTTTGAAG AGCAGTGCAT GGAGGAGTAT 
TTCAGAGAGA AAGATGACCG CTTCAATTCC TCCAATGACG AAAGGATACG TGTGACATCT 
GAAAGGGTGG AGAACATGGC GATGCGATTA GAGGAGGTGA ATGAGAGGGA ACATTTTATG 
AAAGCGTCCT TGCAGACTGT AGACATCCGG CTCGCCCAGA TGGAGGAGAT GATCGGCCGC 
ATTGCTGTGG CCCTAGAGAG AGTTGCTGGA ATGGACAGAG GCGAAGTTAA CAAAGCCCGG 
TCCAGGACAT CATCAGACTG CACTGATACC AATTACATCC TGCGCCAAAG CAGTTTCAAC 
AGCCAAGAAG GCAATTCCTA CAGGCTACAG GAGTCTCTGG AGCAGGGGGG CGACGAGTCC 
ATTTCTCCTA CTTCCCCAAC CGCCCTGGCT CCCCGTGTCC GCAGTCACTC CTTCTACGTG 
AGCCACTCTT CCAAAGACAG GAGTGGGGCG GACCGTGGAG AGGGCTTCTT TAAAGACAAG 
TTGTTCAGCC TCCACCGGGC CAACAGCTCA CAGTCTGTGT CCTCAGGAGC AGGTCCCAAA 
GAGTCCAAGC CAACCCCTTT GAATACTTTA TCCGTGCAGC AGCAGCTCCG CCCTTCTTCC 
TGCATAGACA TCTATGTTTC TGCCTCAGAG GACGTCCCGC CCACTGAGAG CTTCTTGGAT 
TCGGTTCGGA CAGTTCCAAC TCTGGCTCGA GACTCATCTC TTCATTCGGA GATCATGGAG 
GCGGTGCTCT CAGGGGGACG GGACTGTAGT GGAAGGGCAG GCGGCAGCGA AAGACAGTCG 
GATGGTACAG TCCTGTTTGA GGACAGCGCC GCCGCTGACC TATCGCTCTG CTCTGCCCAC 
CTGCTGCCGG ATTCCTTGCC CCCCTGGGAC CTGGACCCCT CACCGCCACC ATCAGCTGGT 
GTACTAGAGC GGTCCAAAAG TAGTCGCTTC CTGTCTACAG CAGGGCCTCT GTTTCTGGAC 
GAGCCTCATT TGGTTAAGTC ACACAGTCTG ATGTTCACAT CACGTGGGTA CTATGGTGGC 
ATGGGTGTGC AGGTGAAGGC GGCCGAATAC ACCAGCATCA CAGATTGCAT CGACACCCGT 
TGCGTCTCAA CCCCTTACCC TGTGCCAGAG CGGTCAGACT CACCTGGAGG ATCTTTCACC 
TTCGATAAGC CTCAAGACCT TGGCGTCTCG CACCCTGAGA GAGACGCTGA GCTCAGCCAC 
GCTGAGTCTG ACTTAGAGGA GCCAGCAGAG GGTTCGGGAG ACACATGTAA GGTGGGTCAG 
AGTAGCAGTA GTGGGGTAAT AGGTGCAGAC CTGGGCCTGG GCCTCGCACT AGGCCCGTTT 
TGCTCACCCA TCTCTAGACT AGAGCGAGCA AACAGCTGCT CCTCTTCAGA GGAGTCGCAC 
TCCAACATCT ACTCTCGAAA GAGCTTCTCA ATAAGCGAGA GAATGGACAA GGGCCGGGGG 
AGCTCCAGGA ACCCTTTCCA GAAGGCCAGA ACCGGGGCGA GGCTGGAGGG TAAGACAGAC 
TCACTGTCTA TGAGGAAAAT GGCCAAACCG TCAGCTTTCA GAAGTTTTGA CAGCAGACAC 
AACTACACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm3 ( XM_021476468.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332143.1 Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X2, mRNA.

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CloneID	ODa142787
Clone ID Related Accession (Same CDS sequence)	XM_021476468.1
Accession Version	XM_021476468.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily M member 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAAAA GAAAATGGGA GGGGAGGAGA GAAGGCTTCA AGGTGAGCTC CACGAGGGAG 
GAGGCACAGG GGCCTGGACG CCCCTCCACC AGAGGGCGCT TCTCCGAATC TTGGAAGAGA 
CTGAGCTCCA GACAGGGGTC TACCAAGAGG TCAGGACTGG CCTCCCAGCA AGCTGCGGCA 
CAGAAATCAT GGATAGAAAG AGCGTTCAGC AAGAGAGAAT GTGTGCACAT TATTGTAAGC 
ACCAAAGACC ACCATAGGTG CTGTTGTGGC CGTCTAATTG GTCAACATGT TGGCCTGCCC 
CCCAGCATCT CATCCAATCA GAATGAGAAG TCAGAACGCG TGCCAAAGAA TGACAGCTTG 
TCAGAGAAGT GGTCCATCAG CAAGCACACC CAGCTCAGTC CCACCGACGC TTTTGGGACC 
ATCGAGTTCC AGGGAGGAGG ACACTCTAAT AAAGCAATGT ATGTACGAGT GTCTTACGAC 
ACAAAACCAG ATTTACTGCT GCATCTGATG ACAAAGGAGT GGCAACTGGA GCTTCCCAAG 
CTGCTCATCT CTGTTCACGG AGGACTTCAA AACTTTGAGT TGCAGCCCAA ACTAAAGCAA 
GTCTTCGGCA AAGGTCTTAT AAAGGCTGCA ATGACTACCG GAGCCTGGAT CTTCACTGGA 
GGAGTTAATA CTGGAGTCAT CAGACATGTT GGTGATGCTC TGAAAGATCA TGCTTCCAAA 
TCAAGAGGGA AGATCTGCAC TATCGGCATT GCCCCCTGGG GGATTGTGGA GAACCAGGAA 
GACCTGGTGG GCAAAGATGT GGTCCGGCCA TACCAGACGA TGTCTAACCC TCTTAGCAAG 
CTAACAGTAC TCAACAGCCT ACACTCACAC TTCATTTTGG CTGATAATGG CACCACCGGA 
AAGTACGGAG CAGAAGTCAA ACTGCGCAGG CAGCTGGAGA AGCACATCTC TCTGCAAAAG 
ATTAACACAC GGATTGGACA GGGTGTTCCA GTAGTGGCTC TGATAGTGGA GGGGGGTCCG 
AATGTCATCT CTATCGTCCT GGAGTACCTA AGAGACACCC CTCCGGTGCC TGTTGTGGTG 
TGTGACGGCA GCGGTCGAGC CTCTGACATC CTGGCCTTTG GGCACAAGTA CTCAGAGGAG 
GGCGGAATCA TAAACGAATC TTTGAGAGAC CAGTTATTAG TCACCATTCA GAAGACGTTC 
ACATACAGCC GGACGCAGGC CCAGCATCTC TTCATCATTC TGATGGAGTG CATGAAGAAG 
AAAGAACTGA TCACAGTGTT TCGGATGGGA TCAGAGGGAC ACCAGGACAT CGATCTAGCC 
ATTCTAACAG CTCTTCTGAA AGGAGCGAAT GCATCAGCTC CAGATCAGCT AAGTCTGGCT 
TTGGCCTGGA ACAGAGTTGA CATCGCCCGG AGCCAGATCT TCATTTACGG ACAGCAGTGG 
CCTGTTGGCT CTCTGGAGCA GTCTATGCTG GACGCGCTGG TGCTGGACCG AGTGGACTTT 
GTAAAGCTGT TGATTGAGAA CGGAGTGAGC ATGCACCGTT TCCTGACACT CTCCAGACTT 
GAAGAGCTTT ATAATACGAG GCACGGACCA TCCAACACCT TGTACCACCT TGTCAGAGAT 
GTGAAAAAGG GTAACCTGCC CCCCGACTAC CGCATTAGCC TGATTGACAT CGGATTGGTT 
ATTGAATACC TCATGGGTGG AGCCTATCGC TGCAATTACA CCAGGAAGAG ATTTCGGACC 
CTCTATCACA ACCTCTTTGG ACCCAAAAGA CCAAAAGCCC TGAAGCTGCT TGGAATGGAG 
GATGACATGC CCATTAGACG AGGGCGTCAG AAGACTACGC GGAAGCGAGA GGAGGAAGTA 
GATATTGACC TGGATGACCC AGAAATCAAT CATTTCCCTT TCCCATTCCA TGAGCTGATG 
GTTTGGGCTG TGCTGATGAA ACGGCAGAAG ATGGCACTGT TCTTCTGGCA GCATGGAGAG 
GAGGCCATGG CTAAAGCGCT GGTGGCCTGC AAGCTCTGTA AGGCAATGGC TCACGAAGCT 
TCTGAGAACG ACATGGTCGA TGACATTTCA CAGGAGCTCA ATCAAAACTC AAGGGAGTTT 
GGTCAGCTGG CTGTTGAGCT GCTCGATCAG TCCTATAAGC AGGATGAACA GATGGCTATG 
AAGCTGTTGA CCTACGAGCT GAAGAACTGG AGTAATGCCA CCTGTCTGCA GCTGGCAGTG 
GCTGCCAAGC ACCGAGACTT CATCGCCCAC ACCTGTAGCC AGATGCTGCT CACAGACATG 
TGGATGGGAC GTCTGCGCAT GCGCAAGAAC TCTGGCCTGA AGGTTATCTT GGGCCTGCTT 
CTCCCCCCTT CCATATTGAG TCTGGAGTTT AAAAATAAAG ACGAGATGTC ATACATGCCT 
CAGGATCAGG ACACATATCT GCAGGAGAAG GATGTGGATG AGCCCGAGAA ACAAGCTAAG 
GAGAAAGAGG AGGAGGACAT GGAGTTCACA GTAAGATCTT ACTGTGAGAC GCAGTACAAC 
TCCGTGGCTA TGTTGGGAAA CGTCAGTTCA GAAGCATCGC GCAAGAAACA GGTGGAGGAG 
GTTCAGAACC GGCATCGGCT CATTCCATTG GGTCGCAAAA TATATGAGTT TTACAACGCC 
CCTATCGTCA AGTTCTGGTT TCACACGCTG GCGTATGTAG GATACTTGAT GCTCTTCAAC 
TATATTGTGC TGGTGAAGAT GGACCTGTGG CCTTCTCCTC AGGAGTGGAT TGTTATTGCC 
TACATATTCA CCAATGGCAT CGAGAAGATG AGAGAGATCC TGATGTCCGA ACCTGGGAAG 
CTGCTTCAGA AGGTGAAAGT GTGGTTACAG GAGTACTGGA ACATCACTGA CCTCATGGCC 
ATCCTCATCT TCTCCATCGG GATGGTGCTT CGTCTGCAGG ACCCGCCGCT GATGAGCTAC 
GGCAGGGTCA TTTACTGCGT CAATATCATT TATTGGTACA TACGACTCCT CGACATCTTC 
GGAGTGAACA AATACCTGGG TCCATACGTC ATGATGATCG GCAAGATGAT GATTGACATG 
ATGTATTTTG TGATCATCAT GTTGGTGGTG CTGATGAGTT TCGGTGTGGC GCGTCAGGCC 
ATCCTTAATC CCAATGAAGA CCCATCCTGG ATGCTGGCAC GCAATATCTT CTTCATGCCA 
TACTGGATGA TCTACGGAGA GGTGTTTGCT GACCAAATCG ACCATGTGAA TAGTAGGAAG 
CTACATCCCA AAGTGAATGA CAAACTATGG CTGGTCGAAA ATTACATGCG CACTCAAGGA 
ACTGGGCGTT ACAGTGGTCC ATCCCATATA GCTCCTTGTG GTCAGAACAT CACCACAGAA 
GAAGGAGTTG TCATGCCTCT TCCACCCTGC AAAACTGGAG CTTGGATTGT CCCTGCCATA 
ATGGCCTGCT ACCTGCTGGT GGCCAACATC CTGCTAGTTA ACTTGCTCAT TGCTGTCTTT 
AATAACACTT TCTTTGAAGT CAAGTCCATT TCCAACCAGG TGTGGAAGTT TCAGAGGTAT 
CAGCTGATCA TGACCTTCCA TGAACGCCCG GTCCTTCCTC CTCCACTCAT CATCTTCAGC 
CACATAACTA TGGTTCTCAA ACACCTGTGC TGTCGCTGGA GGAAGCACGA TGAGGATGAA 
CGGGACTATG GGCTGAAGCT TTTCATCACT GAAGATGAAC TGAAGAAAGT GCATGACTTT 
GAAGAGCAGT GCATGGAGGA GTATTTCAGA GAGAAAGATG ACCGCTTCAA TTCCTCCAAT 
GACGAAAGGA TACGTGTGAC ATCTGAAAGG GTGGAGAACA TGGCGATGCG ATTAGAGGAG 
GTGAATGAGA GGGAACATTT TATGAAAGCG TCCTTGCAGA CTGTAGACAT CCGGCTCGCC 
CAGATGGAGG AGATGATCGG CCGCATTGCT GTGGCCCTAG AGAGAGTTGC TGGAATGGAC 
AGAGGCGAAG TTAACAAAGC CCGGTCCAGG ACATCATCAG ACTGCACTGA TACCAATTAC 
ATCCTGCGCC AAAGCAGTTT CAACAGCCAA GAAGGCAATT CCTACAGGCT ACAGGAGTCT 
CTGGAGCAGG GGGGCGACGA GTCCATTTCT CCTACTTCCC CAACCGCCCT GGCTCCCCGT 
GTCCGCAGTC ACTCCTTCTA CGTGAGCCAC TCTTCCAAAG ACAGGAGTGG GGCGGACCGT 
GGAGAGGGCT TCTTTAAAGA CAAGTTGTTC AGCCTCCACC GGGCCAACAG CTCACAGTCT 
GTGTCCTCAG GAGCAGGTCC CAAAGAGTCC AAGCCAACCC CTTTGAATAC TTTATCCGTG 
CAGCAGCAGC TCCGCCCTTC TTCCTGCATA GACATCTATG TTTCTGCCTC AGAGGACGTC 
CCGCCCACTG AGAGCTTCTT GGATTCGGTT CGGACAGTTC CAACTCTGGC TCGAGACTCA 
TCTCTTCATT CGGAGATCAT GGAGGCGGTG CTCTCAGGGG GACGGGACTG TAGTGGAAGG 
GCAGGCGGCA GCGAAAGACA GTCGGATGGT ACAGTCCTGT TTGAGGACAG CGCCGCCGCT 
GACCTATCGC TCTGCTCTGC CCACCTGCTG CCGGATTCCT TGCCCCCCTG GGACCTGGAC 
CCCTCACCGC CACCATCAGC TGGTGTACTA GAGCGGTCCA AAAGTAGTCG CTTCCTGTCT 
ACAGCAGGGC CTCTGTTTCT GGACGAGCCT CATTTGGTTA AGTCACACAG TCTGATGTTC 
ACATCACGTG GGTACTATGG TGGCATGGGT GTGCAGGTGA AGGCGGCCGA ATACACCAGC 
ATCACAGATT GCATCGACAC CCGTTGCGTC TCAACCCCTT ACCCTGTGCC AGAGCGGTCA 
GACTCACCTG GAGGATCTTT CACCTTCGAT AAGCCTCAAG ACCTTGGCGT CTCGCACCCT 
GAGAGAGACG CTGAGCTCAG CCACGCTGAG TCTGACTTAG AGGAGCCAGC AGAGGGTTCG 
GGAGACACAT GTAAGGTGGG TCAGAGTAGC AGTAGTGGGG TAATAGGTGC AGACCTGGGC 
CTGGGCCTCG CACTAGGCCC GTTTTGCTCA CCCATCTCTA GACTAGAGCG AGCAAACAGC 
TGCTCCTCTT CAGAGGAGTC GCACTCCAAC ATCTACTCTC GAAAGAGCTT CTCAATAAGC 
GAGAGAATGG ACAAGGGCCG GGGGAGCTCC AGGAACCCTT TCCAGAAGGC CAGAACCGGG 
GCGAGGCTGG AGGGTAAGAC AGACTCACTG TCTATGAGGA AAATGGCCAA ACCGTCAGCT 
TTCAGAAGTT TTGACAGCAG ACACAACTAC ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332143.1
CDS	378..5693
Translation	MTKRKWEGRREGFKVSSTREEAQGPGRPSTRGRFSESWKRLSSRQGSTKRSGLASQQAAAQKSWIERAFSKRECVHIIVSTKDHHRCCCGRLIGQHVGLPPSISSNQNEKSERVPKNDSLSEKWSISKHTQLSPTDAFGTIEFQGGGHSNKAMYVRVSYDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLVGKDVVRPYQTMSNPLSKLTVLNSLHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGIINESLRDQLLVTIQKTFTYSRTQAQHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQSMLDALVLDRVDFVKLLIENGVSMHRFLTLSRLEELYNTRHGPSNTLYHLVRDVKKGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDMPIRRGRQKTTRKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNQNSREFGQLAVELLDQSYKQDEQMAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGLLLPPSILSLEFKNKDEMSYMPQDQDTYLQEKDVDEPEKQAKEKEEEDMEFTVRSYCETQYNSVAMLGNVSSEASRKKQVEEVQNRHRLIPLGRKIYEFYNAPIVKFWFHTLAYVGYLMLFNYIVLVKMDLWPSPQEWIVIAYIFTNGIEKMREILMSEPGKLLQKVKVWLQEYWNITDLMAILIFSIGMVLRLQDPPLMSYGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDHVNSRKLHPKVNDKLWLVENYMRTQGTGRYSGPSHIAPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHITMVLKHLCCRWRKHDEDERDYGLKLFITEDELKKVHDFEEQCMEEYFREKDDRFNSSNDERIRVTSERVENMAMRLEEVNEREHFMKASLQTVDIRLAQMEEMIGRIAVALERVAGMDRGEVNKARSRTSSDCTDTNYILRQSSFNSQEGNSYRLQESLEQGGDESISPTSPTALAPRVRSHSFYVSHSSKDRSGADRGEGFFKDKLFSLHRANSSQSVSSGAGPKESKPTPLNTLSVQQQLRPSSCIDIYVSASEDVPPTESFLDSVRTVPTLARDSSLHSEIMEAVLSGGRDCSGRAGGSERQSDGTVLFEDSAAADLSLCSAHLLPDSLPPWDLDPSPPPSAGVLERSKSSRFLSTAGPLFLDEPHLVKSHSLMFTSRGYYGGMGVQVKAAEYTSITDCIDTRCVSTPYPVPERSDSPGGSFTFDKPQDLGVSHPERDAELSHAESDLEEPAEGSGDTCKVGQSSSSGVIGADLGLGLALGPFCSPISRLERANSCSSSEESHSNIYSRKSFSISERMDKGRGSSRNPFQKARTGARLEGKTDSLSMRKMAKPSAFRSFDSRHNYT

Target ORF information:

RefSeq Version	XM_021476468.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476468.1

ORF Insert Sequence:

ATGACAAAAA GAAAATGGGA GGGGAGGAGA GAAGGCTTCA AGGTGAGCTC CACGAGGGAG 
GAGGCACAGG GGCCTGGACG CCCCTCCACC AGAGGGCGCT TCTCCGAATC TTGGAAGAGA 
CTGAGCTCCA GACAGGGGTC TACCAAGAGG TCAGGACTGG CCTCCCAGCA AGCTGCGGCA 
CAGAAATCAT GGATAGAAAG AGCGTTCAGC AAGAGAGAAT GTGTGCACAT TATTGTAAGC 
ACCAAAGACC ACCATAGGTG CTGTTGTGGC CGTCTAATTG GTCAACATGT TGGCCTGCCC 
CCCAGCATCT CATCCAATCA GAATGAGAAG TCAGAACGCG TGCCAAAGAA TGACAGCTTG 
TCAGAGAAGT GGTCCATCAG CAAGCACACC CAGCTCAGTC CCACCGACGC TTTTGGGACC 
ATCGAGTTCC AGGGAGGAGG ACACTCTAAT AAAGCAATGT ATGTACGAGT GTCTTACGAC 
ACAAAACCAG ATTTACTGCT GCATCTGATG ACAAAGGAGT GGCAACTGGA GCTTCCCAAG 
CTGCTCATCT CTGTTCACGG AGGACTTCAA AACTTTGAGT TGCAGCCCAA ACTAAAGCAA 
GTCTTCGGCA AAGGTCTTAT AAAGGCTGCA ATGACTACCG GAGCCTGGAT CTTCACTGGA 
GGAGTTAATA CTGGAGTCAT CAGACATGTT GGTGATGCTC TGAAAGATCA TGCTTCCAAA 
TCAAGAGGGA AGATCTGCAC TATCGGCATT GCCCCCTGGG GGATTGTGGA GAACCAGGAA 
GACCTGGTGG GCAAAGATGT GGTCCGGCCA TACCAGACGA TGTCTAACCC TCTTAGCAAG 
CTAACAGTAC TCAACAGCCT ACACTCACAC TTCATTTTGG CTGATAATGG CACCACCGGA 
AAGTACGGAG CAGAAGTCAA ACTGCGCAGG CAGCTGGAGA AGCACATCTC TCTGCAAAAG 
ATTAACACAC GGATTGGACA GGGTGTTCCA GTAGTGGCTC TGATAGTGGA GGGGGGTCCG 
AATGTCATCT CTATCGTCCT GGAGTACCTA AGAGACACCC CTCCGGTGCC TGTTGTGGTG 
TGTGACGGCA GCGGTCGAGC CTCTGACATC CTGGCCTTTG GGCACAAGTA CTCAGAGGAG 
GGCGGAATCA TAAACGAATC TTTGAGAGAC CAGTTATTAG TCACCATTCA GAAGACGTTC 
ACATACAGCC GGACGCAGGC CCAGCATCTC TTCATCATTC TGATGGAGTG CATGAAGAAG 
AAAGAACTGA TCACAGTGTT TCGGATGGGA TCAGAGGGAC ACCAGGACAT CGATCTAGCC 
ATTCTAACAG CTCTTCTGAA AGGAGCGAAT GCATCAGCTC CAGATCAGCT AAGTCTGGCT 
TTGGCCTGGA ACAGAGTTGA CATCGCCCGG AGCCAGATCT TCATTTACGG ACAGCAGTGG 
CCTGTTGGCT CTCTGGAGCA GTCTATGCTG GACGCGCTGG TGCTGGACCG AGTGGACTTT 
GTAAAGCTGT TGATTGAGAA CGGAGTGAGC ATGCACCGTT TCCTGACACT CTCCAGACTT 
GAAGAGCTTT ATAATACGAG GCACGGACCA TCCAACACCT TGTACCACCT TGTCAGAGAT 
GTGAAAAAGG GTAACCTGCC CCCCGACTAC CGCATTAGCC TGATTGACAT CGGATTGGTT 
ATTGAATACC TCATGGGTGG AGCCTATCGC TGCAATTACA CCAGGAAGAG ATTTCGGACC 
CTCTATCACA ACCTCTTTGG ACCCAAAAGA CCAAAAGCCC TGAAGCTGCT TGGAATGGAG 
GATGACATGC CCATTAGACG AGGGCGTCAG AAGACTACGC GGAAGCGAGA GGAGGAAGTA 
GATATTGACC TGGATGACCC AGAAATCAAT CATTTCCCTT TCCCATTCCA TGAGCTGATG 
GTTTGGGCTG TGCTGATGAA ACGGCAGAAG ATGGCACTGT TCTTCTGGCA GCATGGAGAG 
GAGGCCATGG CTAAAGCGCT GGTGGCCTGC AAGCTCTGTA AGGCAATGGC TCACGAAGCT 
TCTGAGAACG ACATGGTCGA TGACATTTCA CAGGAGCTCA ATCAAAACTC AAGGGAGTTT 
GGTCAGCTGG CTGTTGAGCT GCTCGATCAG TCCTATAAGC AGGATGAACA GATGGCTATG 
AAGCTGTTGA CCTACGAGCT GAAGAACTGG AGTAATGCCA CCTGTCTGCA GCTGGCAGTG 
GCTGCCAAGC ACCGAGACTT CATCGCCCAC ACCTGTAGCC AGATGCTGCT CACAGACATG 
TGGATGGGAC GTCTGCGCAT GCGCAAGAAC TCTGGCCTGA AGGTTATCTT GGGCCTGCTT 
CTCCCCCCTT CCATATTGAG TCTGGAGTTT AAAAATAAAG ACGAGATGTC ATACATGCCT 
CAGGATCAGG ACACATATCT GCAGGAGAAG GATGTGGATG AGCCCGAGAA ACAAGCTAAG 
GAGAAAGAGG AGGAGGACAT GGAGTTCACA GTAAGATCTT ACTGTGAGAC GCAGTACAAC 
TCCGTGGCTA TGTTGGGAAA CGTCAGTTCA GAAGCATCGC GCAAGAAACA GGTGGAGGAG 
GTTCAGAACC GGCATCGGCT CATTCCATTG GGTCGCAAAA TATATGAGTT TTACAACGCC 
CCTATCGTCA AGTTCTGGTT TCACACGCTG GCGTATGTAG GATACTTGAT GCTCTTCAAC 
TATATTGTGC TGGTGAAGAT GGACCTGTGG CCTTCTCCTC AGGAGTGGAT TGTTATTGCC 
TACATATTCA CCAATGGCAT CGAGAAGATG AGAGAGATCC TGATGTCCGA ACCTGGGAAG 
CTGCTTCAGA AGGTGAAAGT GTGGTTACAG GAGTACTGGA ACATCACTGA CCTCATGGCC 
ATCCTCATCT TCTCCATCGG GATGGTGCTT CGTCTGCAGG ACCCGCCGCT GATGAGCTAC 
GGCAGGGTCA TTTACTGCGT CAATATCATT TATTGGTACA TACGACTCCT CGACATCTTC 
GGAGTGAACA AATACCTGGG TCCATACGTC ATGATGATCG GCAAGATGAT GATTGACATG 
ATGTATTTTG TGATCATCAT GTTGGTGGTG CTGATGAGTT TCGGTGTGGC GCGTCAGGCC 
ATCCTTAATC CCAATGAAGA CCCATCCTGG ATGCTGGCAC GCAATATCTT CTTCATGCCA 
TACTGGATGA TCTACGGAGA GGTGTTTGCT GACCAAATCG ACCATGTGAA TAGTAGGAAG 
CTACATCCCA AAGTGAATGA CAAACTATGG CTGGTCGAAA ATTACATGCG CACTCAAGGA 
ACTGGGCGTT ACAGTGGTCC ATCCCATATA GCTCCTTGTG GTCAGAACAT CACCACAGAA 
GAAGGAGTTG TCATGCCTCT TCCACCCTGC AAAACTGGAG CTTGGATTGT CCCTGCCATA 
ATGGCCTGCT ACCTGCTGGT GGCCAACATC CTGCTAGTTA ACTTGCTCAT TGCTGTCTTT 
AATAACACTT TCTTTGAAGT CAAGTCCATT TCCAACCAGG TGTGGAAGTT TCAGAGGTAT 
CAGCTGATCA TGACCTTCCA TGAACGCCCG GTCCTTCCTC CTCCACTCAT CATCTTCAGC 
CACATAACTA TGGTTCTCAA ACACCTGTGC TGTCGCTGGA GGAAGCACGA TGAGGATGAA 
CGGGACTATG GGCTGAAGCT TTTCATCACT GAAGATGAAC TGAAGAAAGT GCATGACTTT 
GAAGAGCAGT GCATGGAGGA GTATTTCAGA GAGAAAGATG ACCGCTTCAA TTCCTCCAAT 
GACGAAAGGA TACGTGTGAC ATCTGAAAGG GTGGAGAACA TGGCGATGCG ATTAGAGGAG 
GTGAATGAGA GGGAACATTT TATGAAAGCG TCCTTGCAGA CTGTAGACAT CCGGCTCGCC 
CAGATGGAGG AGATGATCGG CCGCATTGCT GTGGCCCTAG AGAGAGTTGC TGGAATGGAC 
AGAGGCGAAG TTAACAAAGC CCGGTCCAGG ACATCATCAG ACTGCACTGA TACCAATTAC 
ATCCTGCGCC AAAGCAGTTT CAACAGCCAA GAAGGCAATT CCTACAGGCT ACAGGAGTCT 
CTGGAGCAGG GGGGCGACGA GTCCATTTCT CCTACTTCCC CAACCGCCCT GGCTCCCCGT 
GTCCGCAGTC ACTCCTTCTA CGTGAGCCAC TCTTCCAAAG ACAGGAGTGG GGCGGACCGT 
GGAGAGGGCT TCTTTAAAGA CAAGTTGTTC AGCCTCCACC GGGCCAACAG CTCACAGTCT 
GTGTCCTCAG GAGCAGGTCC CAAAGAGTCC AAGCCAACCC CTTTGAATAC TTTATCCGTG 
CAGCAGCAGC TCCGCCCTTC TTCCTGCATA GACATCTATG TTTCTGCCTC AGAGGACGTC 
CCGCCCACTG AGAGCTTCTT GGATTCGGTT CGGACAGTTC CAACTCTGGC TCGAGACTCA 
TCTCTTCATT CGGAGATCAT GGAGGCGGTG CTCTCAGGGG GACGGGACTG TAGTGGAAGG 
GCAGGCGGCA GCGAAAGACA GTCGGATGGT ACAGTCCTGT TTGAGGACAG CGCCGCCGCT 
GACCTATCGC TCTGCTCTGC CCACCTGCTG CCGGATTCCT TGCCCCCCTG GGACCTGGAC 
CCCTCACCGC CACCATCAGC TGGTGTACTA GAGCGGTCCA AAAGTAGTCG CTTCCTGTCT 
ACAGCAGGGC CTCTGTTTCT GGACGAGCCT CATTTGGTTA AGTCACACAG TCTGATGTTC 
ACATCACGTG GGTACTATGG TGGCATGGGT GTGCAGGTGA AGGCGGCCGA ATACACCAGC 
ATCACAGATT GCATCGACAC CCGTTGCGTC TCAACCCCTT ACCCTGTGCC AGAGCGGTCA 
GACTCACCTG GAGGATCTTT CACCTTCGAT AAGCCTCAAG ACCTTGGCGT CTCGCACCCT 
GAGAGAGACG CTGAGCTCAG CCACGCTGAG TCTGACTTAG AGGAGCCAGC AGAGGGTTCG 
GGAGACACAT GTAAGGTGGG TCAGAGTAGC AGTAGTGGGG TAATAGGTGC AGACCTGGGC 
CTGGGCCTCG CACTAGGCCC GTTTTGCTCA CCCATCTCTA GACTAGAGCG AGCAAACAGC 
TGCTCCTCTT CAGAGGAGTC GCACTCCAAC ATCTACTCTC GAAAGAGCTT CTCAATAAGC 
GAGAGAATGG ACAAGGGCCG GGGGAGCTCC AGGAACCCTT TCCAGAAGGC CAGAACCGGG 
GCGAGGCTGG AGGGTAAGAC AGACTCACTG TCTATGAGGA AAATGGCCAA ACCGTCAGCT 
TTCAGAAGTT TTGACAGCAG ACACAACTAC ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm3 ( XM_021476469.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332144.1 Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X3, mRNA.

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CloneID	ODa142788
Clone ID Related Accession (Same CDS sequence)	XM_021476469.1
Accession Version	XM_021476469.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5316bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily M member 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAAAA GAAAATGGGA GGGGAGGAGA GAAGGCTTCA AGGTGAGCTC CACGAGGGAG 
GAGGCACAGG GGCCTGGACG CCCCTCCACC AGAGGGCGCT TCTCCGAATC TTGGAAGAGA 
CTGAGCTCCA GACAGGGGTC TACCAAGAGG TCAGGACTGG CCTCCCAGCA AGCTGCGGCA 
CAGAAATCAT GGATAGAAAG AGCGTTCAGC AAGAGAGAAT GTGTGCACAT TATTGTAAGC 
ACCAAAGACC ACCATAGGTG CTGTTGTGGC CGTCTAATTG GTCAACATGT TGGCCTGCCC 
CCCAGCATCT CATCCAATCA GAATGAGAAG TCAGAACGCG TGCCAAAGAA TGACAGCTTG 
TCAGAGAAGT GGTCCATCAG CAAGCACACC CAGCTCAGTC CCACCGACGC TTTTGGGACC 
ATCGAGTTCC AGGGAGGAGG ACACTCTAAT AAAGCAATGT ATGTACGAGT GTCTTACGAC 
ACAAAACCAG ATTTACTGCT GCATCTGATG ACAAAGGAGT GGCAACTGGA GCTTCCCAAG 
CTGCTCATCT CTGTTCACGG AGGACTTCAA AACTTTGAGT TGCAGCCCAA ACTAAAGCAA 
GTCTTCGGCA AAGGTCTTAT AAAGGCTGCA ATGACTACCG GAGCCTGGAT CTTCACTGGA 
GGAGTTAATA CTGGAGTCAT CAGACATGTT GGTGATGCTC TGAAAGATCA TGCTTCCAAA 
TCAAGAGGGA AGATCTGCAC TATCGGCATT GCCCCCTGGG GGATTGTGGA GAACCAGGAA 
GACCTGGTGG GCAAAGATGT GGTCCGGCCA TACCAGACGA TGTCTAACCC TCTTAGCAAG 
CTAACAGTAC TCAACAGCCT ACACTCACAC TTCATTTTGG CTGATAATGG CACCACCGGA 
AAGTACGGAG CAGAAGTCAA ACTGCGCAGG CAGCTGGAGA AGCACATCTC TCTGCAAAAG 
ATTAACACAC GGATTGGACA GGGTGTTCCA GTAGTGGCTC TGATAGTGGA GGGGGGTCCG 
AATGTCATCT CTATCGTCCT GGAGTACCTA AGAGACACCC CTCCGGTGCC TGTTGTGGTG 
TGTGACGGCA GCGGTCGAGC CTCTGACATC CTGGCCTTTG GGCACAAGTA CTCAGAGGAG 
GGCGGAATCA TAAACGAATC TTTGAGAGAC CAGTTATTAG TCACCATTCA GAAGACGTTC 
ACATACAGCC GGACGCAGGC CCAGCATCTC TTCATCATTC TGATGGAGTG CATGAAGAAG 
AAAGAACTGA TCACAGTGTT TCGGATGGGA TCAGAGGGAC ACCAGGACAT CGATCTAGCC 
ATTCTAACAG CTCTTCTGAA AGGAGCGAAT GCATCAGCTC CAGATCAGCT AAGTCTGGCT 
TTGGCCTGGA ACAGAGTTGA CATCGCCCGG AGCCAGATCT TCATTTACGG ACAGCAGTGG 
CCTGTTGGCT CTCTGGAGCA GTCTATGCTG GACGCGCTGG TGCTGGACCG AGTGGACTTT 
GTAAAGCTGT TGATTGAGAA CGGAGTGAGC ATGCACCGTT TCCTGACACT CTCCAGACTT 
GAAGAGCTTT ATAATACGAG GCACGGACCA TCCAACACCT TGTACCACCT TGTCAGAGAT 
GTGAAAAAGC AAGAATATCC AGGGTTCAGT TGGATCTACC TCAAGGGTAA CCTGCCCCCC 
GACTACCGCA TTAGCCTGAT TGACATCGGA TTGGTTATTG AATACCTCAT GGGTGGAGCC 
TATCGCTGCA ATTACACCAG GAAGAGATTT CGGACCCTCT ATCACAACCT CTTTGGACCC 
AAAAGACCAA AAGCCCTGAA GCTGCTTGGA ATGGAGGATG ACATGCCCAT TAGACGAGGG 
CGTCAGAAGA CTACGCGGAA GCGAGAGGAG GAAGTAGATA TTGACCTGGA TGACCCAGAA 
ATCAATCATT TCCCTTTCCC ATTCCATGAG CTGATGGTTT GGGCTGTGCT GATGAAACGG 
CAGAAGATGG CACTGTTCTT CTGGCAGCAT GGAGAGGAGG CCATGGCTAA AGCGCTGGTG 
GCCTGCAAGC TCTGTAAGGC AATGGCTCAC GAAGCTTCTG AGAACGACAT GGTCGATGAC 
ATTTCACAGG AGCTCAATCA AAACTCAAGG GAGTTTGGTC AGCTGGCTGT TGAGCTGCTC 
GATCAGTCCT ATAAGCAGGA TGAACAGATG GCTATGAAGC TGTTGACCTA CGAGCTGAAG 
AACTGGAGTA ATGCCACCTG TCTGCAGCTG GCAGTGGCTG CCAAGCACCG AGACTTCATC 
GCCCACACCT GTAGCCAGAT GCTGCTCACA GACATGTGGA TGGGACGTCT GCGCATGCGC 
AAGAACTCTG GCCTGAAGGT TATCTTGGGC CTGCTTCTCC CCCCTTCCAT ATTGAGTCTG 
GAGTTTAAAA ATAAAGACGA GATGTCATAC ATGCCTCAGG ATCAGGACAC ATATCTGCAG 
GAGAAGGATG TGGATGAGCC CGAGAAACAA GCTAAGGAGA AAGAGGAGGA GGACATGGAG 
TTCACAGCTA TGTTGGGAAA CGTCAGTTCA GAAGCATCGC GCAAGAAACA GGTGGAGGAG 
GTTCAGAACC GGCATCGGCT CATTCCATTG GGTCGCAAAA TATATGAGTT TTACAACGCC 
CCTATCGTCA AGTTCTGGTT TCACACGCTG GCGTATGTAG GATACTTGAT GCTCTTCAAC 
TATATTGTGC TGGTGAAGAT GGACCTGTGG CCTTCTCCTC AGGAGTGGAT TGTTATTGCC 
TACATATTCA CCAATGGCAT CGAGAAGATG AGAGAGATCC TGATGTCCGA ACCTGGGAAG 
CTGCTTCAGA AGGTGAAAGT GTGGTTACAG GAGTACTGGA ACATCACTGA CCTCATGGCC 
ATCCTCATCT TCTCCATCGG GATGGTGCTT CGTCTGCAGG ACCCGCCGCT GATGAGCTAC 
GGCAGGGTCA TTTACTGCGT CAATATCATT TATTGGTACA TACGACTCCT CGACATCTTC 
GGAGTGAACA AATACCTGGG TCCATACGTC ATGATGATCG GCAAGATGAT GATTGACATG 
ATGTATTTTG TGATCATCAT GTTGGTGGTG CTGATGAGTT TCGGTGTGGC GCGTCAGGCC 
ATCCTTAATC CCAATGAAGA CCCATCCTGG ATGCTGGCAC GCAATATCTT CTTCATGCCA 
TACTGGATGA TCTACGGAGA GGTGTTTGCT GACCAAATCG ACCATGTGAA TAGTAGGAAG 
CTACATCCCA AAGTGAATGA CAAACTATGG CTGGTCGAAA ATTACATGCG CACTCAAGGA 
ACTGGGCGTT ACAGTGGTCC ATCCCATATA GCTCCTTGTG GTCAGAACAT CACCACAGAA 
GAAGGAGTTG TCATGCCTCT TCCACCCTGC AAAACTGGAG CTTGGATTGT CCCTGCCATA 
ATGGCCTGCT ACCTGCTGGT GGCCAACATC CTGCTAGTTA ACTTGCTCAT TGCTGTCTTT 
AATAACACTT TCTTTGAAGT CAAGTCCATT TCCAACCAGG TGTGGAAGTT TCAGAGGTAT 
CAGCTGATCA TGACCTTCCA TGAACGCCCG GTCCTTCCTC CTCCACTCAT CATCTTCAGC 
CACATAACTA TGGTTCTCAA ACACCTGTGC TGTCGCTGGA GGAAGCACGA TGAGGATGAA 
CGGGACTATG GGCTGAAGCT TTTCATCACT GAAGATGAAC TGAAGAAAGT GCATGACTTT 
GAAGAGCAGT GCATGGAGGA GTATTTCAGA GAGAAAGATG ACCGCTTCAA TTCCTCCAAT 
GACGAAAGGA TACGTGTGAC ATCTGAAAGG GTGGAGAACA TGGCGATGCG ATTAGAGGAG 
GTGAATGAGA GGGAACATTT TATGAAAGCG TCCTTGCAGA CTGTAGACAT CCGGCTCGCC 
CAGATGGAGG AGATGATCGG CCGCATTGCT GTGGCCCTAG AGAGAGTTGC TGGAATGGAC 
AGAGGCGAAG TTAACAAAGC CCGGTCCAGG ACATCATCAG ACTGCACTGA TACCAATTAC 
ATCCTGCGCC AAAGCAGTTT CAACAGCCAA GAAGGCAATT CCTACAGGCT ACAGGAGTCT 
CTGGAGCAGG GGGGCGACGA GTCCATTTCT CCTACTTCCC CAACCGCCCT GGCTCCCCGT 
GTCCGCAGTC ACTCCTTCTA CGTGAGCCAC TCTTCCAAAG ACAGGAGTGG GGCGGACCGT 
GGAGAGGGCT TCTTTAAAGA CAAGTTGTTC AGCCTCCACC GGGCCAACAG CTCACAGTCT 
GTGTCCTCAG GAGCAGGTCC CAAAGAGTCC AAGCCAACCC CTTTGAATAC TTTATCCGTG 
CAGCAGCAGC TCCGCCCTTC TTCCTGCATA GACATCTATG TTTCTGCCTC AGAGGACGTC 
CCGCCCACTG AGAGCTTCTT GGATTCGGTT CGGACAGTTC CAACTCTGGC TCGAGACTCA 
TCTCTTCATT CGGAGATCAT GGAGGCGGTG CTCTCAGGGG GACGGGACTG TAGTGGAAGG 
GCAGGCGGCA GCGAAAGACA GTCGGATGGT ACAGTCCTGT TTGAGGACAG CGCCGCCGCT 
GACCTATCGC TCTGCTCTGC CCACCTGCTG CCGGATTCCT TGCCCCCCTG GGACCTGGAC 
CCCTCACCGC CACCATCAGC TGGTGTACTA GAGCGGTCCA AAAGTAGTCG CTTCCTGTCT 
ACAGCAGGGC CTCTGTTTCT GGACGAGCCT CATTTGGTTA AGTCACACAG TCTGATGTTC 
ACATCACGTG GGTACTATGG TGGCATGGGT GTGCAGGTGA AGGCGGCCGA ATACACCAGC 
ATCACAGATT GCATCGACAC CCGTTGCGTC TCAACCCCTT ACCCTGTGCC AGAGCGGTCA 
GACTCACCTG GAGGATCTTT CACCTTCGAT AAGCCTCAAG ACCTTGGCGT CTCGCACCCT 
GAGAGAGACG CTGAGCTCAG CCACGCTGAG TCTGACTTAG AGGAGCCAGC AGAGGGTTCG 
GGAGACACAT GTAAGGTGGG TCAGAGTAGC AGTAGTGGGG TAATAGGTGC AGACCTGGGC 
CTGGGCCTCG CACTAGGCCC GTTTTGCTCA CCCATCTCTA GACTAGAGCG AGCAAACAGC 
TGCTCCTCTT CAGAGGAGTC GCACTCCAAC ATCTACTCTC GAAAGAGCTT CTCAATAAGC 
GAGAGAATGG ACAAGGGCCG GGGGAGCTCC AGGAACCCTT TCCAGAAGGC CAGAACCGGG 
GCGAGGCTGG AGGGTAAGAC AGACTCACTG TCTATGAGGA AAATGGCCAA ACCGTCAGCT 
TTCAGAAGTT TTGACAGCAG ACACAACTAC ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332144.1
CDS	378..5693
Translation	MTKRKWEGRREGFKVSSTREEAQGPGRPSTRGRFSESWKRLSSRQGSTKRSGLASQQAAAQKSWIERAFSKRECVHIIVSTKDHHRCCCGRLIGQHVGLPPSISSNQNEKSERVPKNDSLSEKWSISKHTQLSPTDAFGTIEFQGGGHSNKAMYVRVSYDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLVGKDVVRPYQTMSNPLSKLTVLNSLHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGIINESLRDQLLVTIQKTFTYSRTQAQHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQSMLDALVLDRVDFVKLLIENGVSMHRFLTLSRLEELYNTRHGPSNTLYHLVRDVKKQEYPGFSWIYLKGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDMPIRRGRQKTTRKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNQNSREFGQLAVELLDQSYKQDEQMAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGLLLPPSILSLEFKNKDEMSYMPQDQDTYLQEKDVDEPEKQAKEKEEEDMEFTAMLGNVSSEASRKKQVEEVQNRHRLIPLGRKIYEFYNAPIVKFWFHTLAYVGYLMLFNYIVLVKMDLWPSPQEWIVIAYIFTNGIEKMREILMSEPGKLLQKVKVWLQEYWNITDLMAILIFSIGMVLRLQDPPLMSYGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDHVNSRKLHPKVNDKLWLVENYMRTQGTGRYSGPSHIAPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHITMVLKHLCCRWRKHDEDERDYGLKLFITEDELKKVHDFEEQCMEEYFREKDDRFNSSNDERIRVTSERVENMAMRLEEVNEREHFMKASLQTVDIRLAQMEEMIGRIAVALERVAGMDRGEVNKARSRTSSDCTDTNYILRQSSFNSQEGNSYRLQESLEQGGDESISPTSPTALAPRVRSHSFYVSHSSKDRSGADRGEGFFKDKLFSLHRANSSQSVSSGAGPKESKPTPLNTLSVQQQLRPSSCIDIYVSASEDVPPTESFLDSVRTVPTLARDSSLHSEIMEAVLSGGRDCSGRAGGSERQSDGTVLFEDSAAADLSLCSAHLLPDSLPPWDLDPSPPPSAGVLERSKSSRFLSTAGPLFLDEPHLVKSHSLMFTSRGYYGGMGVQVKAAEYTSITDCIDTRCVSTPYPVPERSDSPGGSFTFDKPQDLGVSHPERDAELSHAESDLEEPAEGSGDTCKVGQSSSSGVIGADLGLGLALGPFCSPISRLERANSCSSSEESHSNIYSRKSFSISERMDKGRGSSRNPFQKARTGARLEGKTDSLSMRKMAKPSAFRSFDSRHNYT

Target ORF information:

RefSeq Version	XM_021476469.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476469.1

ORF Insert Sequence:

ATGACAAAAA GAAAATGGGA GGGGAGGAGA GAAGGCTTCA AGGTGAGCTC CACGAGGGAG 
GAGGCACAGG GGCCTGGACG CCCCTCCACC AGAGGGCGCT TCTCCGAATC TTGGAAGAGA 
CTGAGCTCCA GACAGGGGTC TACCAAGAGG TCAGGACTGG CCTCCCAGCA AGCTGCGGCA 
CAGAAATCAT GGATAGAAAG AGCGTTCAGC AAGAGAGAAT GTGTGCACAT TATTGTAAGC 
ACCAAAGACC ACCATAGGTG CTGTTGTGGC CGTCTAATTG GTCAACATGT TGGCCTGCCC 
CCCAGCATCT CATCCAATCA GAATGAGAAG TCAGAACGCG TGCCAAAGAA TGACAGCTTG 
TCAGAGAAGT GGTCCATCAG CAAGCACACC CAGCTCAGTC CCACCGACGC TTTTGGGACC 
ATCGAGTTCC AGGGAGGAGG ACACTCTAAT AAAGCAATGT ATGTACGAGT GTCTTACGAC 
ACAAAACCAG ATTTACTGCT GCATCTGATG ACAAAGGAGT GGCAACTGGA GCTTCCCAAG 
CTGCTCATCT CTGTTCACGG AGGACTTCAA AACTTTGAGT TGCAGCCCAA ACTAAAGCAA 
GTCTTCGGCA AAGGTCTTAT AAAGGCTGCA ATGACTACCG GAGCCTGGAT CTTCACTGGA 
GGAGTTAATA CTGGAGTCAT CAGACATGTT GGTGATGCTC TGAAAGATCA TGCTTCCAAA 
TCAAGAGGGA AGATCTGCAC TATCGGCATT GCCCCCTGGG GGATTGTGGA GAACCAGGAA 
GACCTGGTGG GCAAAGATGT GGTCCGGCCA TACCAGACGA TGTCTAACCC TCTTAGCAAG 
CTAACAGTAC TCAACAGCCT ACACTCACAC TTCATTTTGG CTGATAATGG CACCACCGGA 
AAGTACGGAG CAGAAGTCAA ACTGCGCAGG CAGCTGGAGA AGCACATCTC TCTGCAAAAG 
ATTAACACAC GGATTGGACA GGGTGTTCCA GTAGTGGCTC TGATAGTGGA GGGGGGTCCG 
AATGTCATCT CTATCGTCCT GGAGTACCTA AGAGACACCC CTCCGGTGCC TGTTGTGGTG 
TGTGACGGCA GCGGTCGAGC CTCTGACATC CTGGCCTTTG GGCACAAGTA CTCAGAGGAG 
GGCGGAATCA TAAACGAATC TTTGAGAGAC CAGTTATTAG TCACCATTCA GAAGACGTTC 
ACATACAGCC GGACGCAGGC CCAGCATCTC TTCATCATTC TGATGGAGTG CATGAAGAAG 
AAAGAACTGA TCACAGTGTT TCGGATGGGA TCAGAGGGAC ACCAGGACAT CGATCTAGCC 
ATTCTAACAG CTCTTCTGAA AGGAGCGAAT GCATCAGCTC CAGATCAGCT AAGTCTGGCT 
TTGGCCTGGA ACAGAGTTGA CATCGCCCGG AGCCAGATCT TCATTTACGG ACAGCAGTGG 
CCTGTTGGCT CTCTGGAGCA GTCTATGCTG GACGCGCTGG TGCTGGACCG AGTGGACTTT 
GTAAAGCTGT TGATTGAGAA CGGAGTGAGC ATGCACCGTT TCCTGACACT CTCCAGACTT 
GAAGAGCTTT ATAATACGAG GCACGGACCA TCCAACACCT TGTACCACCT TGTCAGAGAT 
GTGAAAAAGC AAGAATATCC AGGGTTCAGT TGGATCTACC TCAAGGGTAA CCTGCCCCCC 
GACTACCGCA TTAGCCTGAT TGACATCGGA TTGGTTATTG AATACCTCAT GGGTGGAGCC 
TATCGCTGCA ATTACACCAG GAAGAGATTT CGGACCCTCT ATCACAACCT CTTTGGACCC 
AAAAGACCAA AAGCCCTGAA GCTGCTTGGA ATGGAGGATG ACATGCCCAT TAGACGAGGG 
CGTCAGAAGA CTACGCGGAA GCGAGAGGAG GAAGTAGATA TTGACCTGGA TGACCCAGAA 
ATCAATCATT TCCCTTTCCC ATTCCATGAG CTGATGGTTT GGGCTGTGCT GATGAAACGG 
CAGAAGATGG CACTGTTCTT CTGGCAGCAT GGAGAGGAGG CCATGGCTAA AGCGCTGGTG 
GCCTGCAAGC TCTGTAAGGC AATGGCTCAC GAAGCTTCTG AGAACGACAT GGTCGATGAC 
ATTTCACAGG AGCTCAATCA AAACTCAAGG GAGTTTGGTC AGCTGGCTGT TGAGCTGCTC 
GATCAGTCCT ATAAGCAGGA TGAACAGATG GCTATGAAGC TGTTGACCTA CGAGCTGAAG 
AACTGGAGTA ATGCCACCTG TCTGCAGCTG GCAGTGGCTG CCAAGCACCG AGACTTCATC 
GCCCACACCT GTAGCCAGAT GCTGCTCACA GACATGTGGA TGGGACGTCT GCGCATGCGC 
AAGAACTCTG GCCTGAAGGT TATCTTGGGC CTGCTTCTCC CCCCTTCCAT ATTGAGTCTG 
GAGTTTAAAA ATAAAGACGA GATGTCATAC ATGCCTCAGG ATCAGGACAC ATATCTGCAG 
GAGAAGGATG TGGATGAGCC CGAGAAACAA GCTAAGGAGA AAGAGGAGGA GGACATGGAG 
TTCACAGCTA TGTTGGGAAA CGTCAGTTCA GAAGCATCGC GCAAGAAACA GGTGGAGGAG 
GTTCAGAACC GGCATCGGCT CATTCCATTG GGTCGCAAAA TATATGAGTT TTACAACGCC 
CCTATCGTCA AGTTCTGGTT TCACACGCTG GCGTATGTAG GATACTTGAT GCTCTTCAAC 
TATATTGTGC TGGTGAAGAT GGACCTGTGG CCTTCTCCTC AGGAGTGGAT TGTTATTGCC 
TACATATTCA CCAATGGCAT CGAGAAGATG AGAGAGATCC TGATGTCCGA ACCTGGGAAG 
CTGCTTCAGA AGGTGAAAGT GTGGTTACAG GAGTACTGGA ACATCACTGA CCTCATGGCC 
ATCCTCATCT TCTCCATCGG GATGGTGCTT CGTCTGCAGG ACCCGCCGCT GATGAGCTAC 
GGCAGGGTCA TTTACTGCGT CAATATCATT TATTGGTACA TACGACTCCT CGACATCTTC 
GGAGTGAACA AATACCTGGG TCCATACGTC ATGATGATCG GCAAGATGAT GATTGACATG 
ATGTATTTTG TGATCATCAT GTTGGTGGTG CTGATGAGTT TCGGTGTGGC GCGTCAGGCC 
ATCCTTAATC CCAATGAAGA CCCATCCTGG ATGCTGGCAC GCAATATCTT CTTCATGCCA 
TACTGGATGA TCTACGGAGA GGTGTTTGCT GACCAAATCG ACCATGTGAA TAGTAGGAAG 
CTACATCCCA AAGTGAATGA CAAACTATGG CTGGTCGAAA ATTACATGCG CACTCAAGGA 
ACTGGGCGTT ACAGTGGTCC ATCCCATATA GCTCCTTGTG GTCAGAACAT CACCACAGAA 
GAAGGAGTTG TCATGCCTCT TCCACCCTGC AAAACTGGAG CTTGGATTGT CCCTGCCATA 
ATGGCCTGCT ACCTGCTGGT GGCCAACATC CTGCTAGTTA ACTTGCTCAT TGCTGTCTTT 
AATAACACTT TCTTTGAAGT CAAGTCCATT TCCAACCAGG TGTGGAAGTT TCAGAGGTAT 
CAGCTGATCA TGACCTTCCA TGAACGCCCG GTCCTTCCTC CTCCACTCAT CATCTTCAGC 
CACATAACTA TGGTTCTCAA ACACCTGTGC TGTCGCTGGA GGAAGCACGA TGAGGATGAA 
CGGGACTATG GGCTGAAGCT TTTCATCACT GAAGATGAAC TGAAGAAAGT GCATGACTTT 
GAAGAGCAGT GCATGGAGGA GTATTTCAGA GAGAAAGATG ACCGCTTCAA TTCCTCCAAT 
GACGAAAGGA TACGTGTGAC ATCTGAAAGG GTGGAGAACA TGGCGATGCG ATTAGAGGAG 
GTGAATGAGA GGGAACATTT TATGAAAGCG TCCTTGCAGA CTGTAGACAT CCGGCTCGCC 
CAGATGGAGG AGATGATCGG CCGCATTGCT GTGGCCCTAG AGAGAGTTGC TGGAATGGAC 
AGAGGCGAAG TTAACAAAGC CCGGTCCAGG ACATCATCAG ACTGCACTGA TACCAATTAC 
ATCCTGCGCC AAAGCAGTTT CAACAGCCAA GAAGGCAATT CCTACAGGCT ACAGGAGTCT 
CTGGAGCAGG GGGGCGACGA GTCCATTTCT CCTACTTCCC CAACCGCCCT GGCTCCCCGT 
GTCCGCAGTC ACTCCTTCTA CGTGAGCCAC TCTTCCAAAG ACAGGAGTGG GGCGGACCGT 
GGAGAGGGCT TCTTTAAAGA CAAGTTGTTC AGCCTCCACC GGGCCAACAG CTCACAGTCT 
GTGTCCTCAG GAGCAGGTCC CAAAGAGTCC AAGCCAACCC CTTTGAATAC TTTATCCGTG 
CAGCAGCAGC TCCGCCCTTC TTCCTGCATA GACATCTATG TTTCTGCCTC AGAGGACGTC 
CCGCCCACTG AGAGCTTCTT GGATTCGGTT CGGACAGTTC CAACTCTGGC TCGAGACTCA 
TCTCTTCATT CGGAGATCAT GGAGGCGGTG CTCTCAGGGG GACGGGACTG TAGTGGAAGG 
GCAGGCGGCA GCGAAAGACA GTCGGATGGT ACAGTCCTGT TTGAGGACAG CGCCGCCGCT 
GACCTATCGC TCTGCTCTGC CCACCTGCTG CCGGATTCCT TGCCCCCCTG GGACCTGGAC 
CCCTCACCGC CACCATCAGC TGGTGTACTA GAGCGGTCCA AAAGTAGTCG CTTCCTGTCT 
ACAGCAGGGC CTCTGTTTCT GGACGAGCCT CATTTGGTTA AGTCACACAG TCTGATGTTC 
ACATCACGTG GGTACTATGG TGGCATGGGT GTGCAGGTGA AGGCGGCCGA ATACACCAGC 
ATCACAGATT GCATCGACAC CCGTTGCGTC TCAACCCCTT ACCCTGTGCC AGAGCGGTCA 
GACTCACCTG GAGGATCTTT CACCTTCGAT AAGCCTCAAG ACCTTGGCGT CTCGCACCCT 
GAGAGAGACG CTGAGCTCAG CCACGCTGAG TCTGACTTAG AGGAGCCAGC AGAGGGTTCG 
GGAGACACAT GTAAGGTGGG TCAGAGTAGC AGTAGTGGGG TAATAGGTGC AGACCTGGGC 
CTGGGCCTCG CACTAGGCCC GTTTTGCTCA CCCATCTCTA GACTAGAGCG AGCAAACAGC 
TGCTCCTCTT CAGAGGAGTC GCACTCCAAC ATCTACTCTC GAAAGAGCTT CTCAATAAGC 
GAGAGAATGG ACAAGGGCCG GGGGAGCTCC AGGAACCCTT TCCAGAAGGC CAGAACCGGG 
GCGAGGCTGG AGGGTAAGAC AGACTCACTG TCTATGAGGA AAATGGCCAA ACCGTCAGCT 
TTCAGAAGTT TTGACAGCAG ACACAACTAC ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm3 ( XM_021476470.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332145.1 Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X4, mRNA.

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CloneID	ODa142789
Clone ID Related Accession (Same CDS sequence)	XM_021476470.1
Accession Version	XM_021476470.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily M member 3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGCC CTTTTCCGGA TGGCGGGAAC GTCCGGCCGG GAGCGATGAA CCAGCTGGAT 
GCTCCTCGGC CACTCAACTG GACCATACGG AAACTGTGCC ATGCAGCCTT CCTCCCGTCT 
GTGCGCCTGC TTAAGGCACA GAAATCATGG ATAGAAAGAG CGTTCAGCAA GAGAGAATGT 
GTGCACATTA TTGTAAGCAC CAAAGACCAC CATAGGTGCT GTTGTGGCCG TCTAATTGGT 
CAACATGTTG GCCTGCCCCC CAGCATCTCA TCCAATCAGA ATGAGAAGTC AGAACGCGTG 
CCAAAGAATG ACAGCTTGTC AGAGAAGTGG TCCATCAGCA AGCACACCCA GCTCAGTCCC 
ACCGACGCTT TTGGGACCAT CGAGTTCCAG GGAGGAGGAC ACTCTAATAA AGCAATGTAT 
GTACGAGTGT CTTACGACAC AAAACCAGAT TTACTGCTGC ATCTGATGAC AAAGGAGTGG 
CAACTGGAGC TTCCCAAGCT GCTCATCTCT GTTCACGGAG GACTTCAAAA CTTTGAGTTG 
CAGCCCAAAC TAAAGCAAGT CTTCGGCAAA GGTCTTATAA AGGCTGCAAT GACTACCGGA 
GCCTGGATCT TCACTGGAGG AGTTAATACT GGAGTCATCA GACATGTTGG TGATGCTCTG 
AAAGATCATG CTTCCAAATC AAGAGGGAAG ATCTGCACTA TCGGCATTGC CCCCTGGGGG 
ATTGTGGAGA ACCAGGAAGA CCTGGTGGGC AAAGATGTGG TCCGGCCATA CCAGACGATG 
TCTAACCCTC TTAGCAAGCT AACAGTACTC AACAGCCTAC ACTCACACTT CATTTTGGCT 
GATAATGGCA CCACCGGAAA GTACGGAGCA GAAGTCAAAC TGCGCAGGCA GCTGGAGAAG 
CACATCTCTC TGCAAAAGAT TAACACACGG ATTGGACAGG GTGTTCCAGT AGTGGCTCTG 
ATAGTGGAGG GGGGTCCGAA TGTCATCTCT ATCGTCCTGG AGTACCTAAG AGACACCCCT 
CCGGTGCCTG TTGTGGTGTG TGACGGCAGC GGTCGAGCCT CTGACATCCT GGCCTTTGGG 
CACAAGTACT CAGAGGAGGG CGGAATCATA AACGAATCTT TGAGAGACCA GTTATTAGTC 
ACCATTCAGA AGACGTTCAC ATACAGCCGG ACGCAGGCCC AGCATCTCTT CATCATTCTG 
ATGGAGTGCA TGAAGAAGAA AGAACTGATC ACAGTGTTTC GGATGGGATC AGAGGGACAC 
CAGGACATCG ATCTAGCCAT TCTAACAGCT CTTCTGAAAG GAGCGAATGC ATCAGCTCCA 
GATCAGCTAA GTCTGGCTTT GGCCTGGAAC AGAGTTGACA TCGCCCGGAG CCAGATCTTC 
ATTTACGGAC AGCAGTGGCC TGTTGGCTCT CTGGAGCAGT CTATGCTGGA CGCGCTGGTG 
CTGGACCGAG TGGACTTTGT AAAGCTGTTG ATTGAGAACG GAGTGAGCAT GCACCGTTTC 
CTGACACTCT CCAGACTTGA AGAGCTTTAT AATACGAGGC ACGGACCATC CAACACCTTG 
TACCACCTTG TCAGAGATGT GAAAAAGCAA GAATATCCAG GGTTCAGTTG GATCTACCTC 
AAGGGTAACC TGCCCCCCGA CTACCGCATT AGCCTGATTG ACATCGGATT GGTTATTGAA 
TACCTCATGG GTGGAGCCTA TCGCTGCAAT TACACCAGGA AGAGATTTCG GACCCTCTAT 
CACAACCTCT TTGGACCCAA AAGACCAAAA GCCCTGAAGC TGCTTGGAAT GGAGGATGAC 
ATGCCCATTA GACGAGGGCG TCAGAAGACT ACGCGGAAGC GAGAGGAGGA AGTAGATATT 
GACCTGGATG ACCCAGAAAT CAATCATTTC CCTTTCCCAT TCCATGAGCT GATGGTTTGG 
GCTGTGCTGA TGAAACGGCA GAAGATGGCA CTGTTCTTCT GGCAGCATGG AGAGGAGGCC 
ATGGCTAAAG CGCTGGTGGC CTGCAAGCTC TGTAAGGCAA TGGCTCACGA AGCTTCTGAG 
AACGACATGG TCGATGACAT TTCACAGGAG CTCAATCAAA ACTCAAGGGA GTTTGGTCAG 
CTGGCTGTTG AGCTGCTCGA TCAGTCCTAT AAGCAGGATG AACAGATGGC TATGAAGCTG 
TTGACCTACG AGCTGAAGAA CTGGAGTAAT GCCACCTGTC TGCAGCTGGC AGTGGCTGCC 
AAGCACCGAG ACTTCATCGC CCACACCTGT AGCCAGATGC TGCTCACAGA CATGTGGATG 
GGACGTCTGC GCATGCGCAA GAACTCTGGC CTGAAGGTTA TCTTGGGCCT GCTTCTCCCC 
CCTTCCATAT TGAGTCTGGA GTTTAAAAAT AAAGACGAGA TGTCATACAT GCCTCAGGAT 
CAGGACACAT ATCTGCAGGA GAAGGATGTG GATGAGCCCG AGAAACAAGC TAAGGAGAAA 
GAGGAGGAGG ACATGGAGTT CACAGTAAGA TCTTACTGTG AGACGCAGTA CAACTCCGTG 
GCTATGTTGG GAAACGTCAG TTCAGAAGCA TCGCGCAAGA AACAGGTGGA GGAGGTTCAG 
AACCGGCATC GGCTCATTCC ATTGGGTCGC AAAATATATG AGTTTTACAA CGCCCCTATC 
GTCAAGTTCT GGTTTCACAC GCTGGCGTAT GTAGGATACT TGATGCTCTT CAACTATATT 
GTGCTGGTGA AGATGGACCT GTGGCCTTCT CCTCAGGAGT GGATTGTTAT TGCCTACATA 
TTCACCAATG GCATCGAGAA GATGAGAGAG ATCCTGATGT CCGAACCTGG GAAGCTGCTT 
CAGAAGGTGA AAGTGTGGTT ACAGGAGTAC TGGAACATCA CTGACCTCAT GGCCATCCTC 
ATCTTCTCCA TCGGGATGGT GCTTCGTCTG CAGGACCCGC CGCTGATGAG CTACGGCAGG 
GTCATTTACT GCGTCAATAT CATTTATTGG TACATACGAC TCCTCGACAT CTTCGGAGTG 
AACAAATACC TGGGTCCATA CGTCATGATG ATCGGCAAGA TGATGATTGA CATGATGTAT 
TTTGTGATCA TCATGTTGGT GGTGCTGATG AGTTTCGGTG TGGCGCGTCA GGCCATCCTT 
AATCCCAATG AAGACCCATC CTGGATGCTG GCACGCAATA TCTTCTTCAT GCCATACTGG 
ATGATCTACG GAGAGGTGTT TGCTGACCAA ATCGACCATG TGAATAGTAG GAAGCTACAT 
CCCAAAGTGA ATGACAAACT ATGGCTGGTC GAAAATTACA TGCGCACTCA AGGAACTGGG 
CGTTACAGTG GTCCATCCCA TATAGCTCCT TGTGGTCAGA ACATCACCAC AGAAGAAGGA 
GTTGTCATGC CTCTTCCACC CTGCAAAACT GGAGCTTGGA TTGTCCCTGC CATAATGGCC 
TGCTACCTGC TGGTGGCCAA CATCCTGCTA GTTAACTTGC TCATTGCTGT CTTTAATAAC 
ACTTTCTTTG AAGTCAAGTC CATTTCCAAC CAGGTGTGGA AGTTTCAGAG GTATCAGCTG 
ATCATGACCT TCCATGAACG CCCGGTCCTT CCTCCTCCAC TCATCATCTT CAGCCACATA 
ACTATGGTTC TCAAACACCT GTGCTGTCGC TGGAGGAAGC ACGATGAGGA TGAACGGGAC 
TATGGGCTGA AGCTTTTCAT CACTGAAGAT GAACTGAAGA AAGTGCATGA CTTTGAAGAG 
CAGTGCATGG AGGAGTATTT CAGAGAGAAA GATGACCGCT TCAATTCCTC CAATGACGAA 
AGGATACGTG TGACATCTGA AAGGGTGGAG AACATGGCGA TGCGATTAGA GGAGGTGAAT 
GAGAGGGAAC ATTTTATGAA AGCGTCCTTG CAGACTGTAG ACATCCGGCT CGCCCAGATG 
GAGGAGATGA TCGGCCGCAT TGCTGTGGCC CTAGAGAGAG TTGCTGGAAT GGACAGAGGC 
GAAGTTAACA AAGCCCGGTC CAGGACATCA TCAGACTGCA CTGATACCAA TTACATCCTG 
CGCCAAAGCA GTTTCAACAG CCAAGAAGGC AATTCCTACA GGCTACAGGA GTCTCTGGAG 
CAGGGGGGCG ACGAGTCCAT TTCTCCTACT TCCCCAACCG CCCTGGCTCC CCGTGTCCGC 
AGTCACTCCT TCTACGTGAG CCACTCTTCC AAAGACAGGA GTGGGGCGGA CCGTGGAGAG 
GGCTTCTTTA AAGACAAGTT GTTCAGCCTC CACCGGGCCA ACAGCTCACA GTCTGTGTCC 
TCAGGAGCAG GTCCCAAAGA GTCCAAGCCA ACCCCTTTGA ATACTTTATC CGTGCAGCAG 
CAGCTCCGCC CTTCTTCCTG CATAGACATC TATGTTTCTG CCTCAGAGGA CGTCCCGCCC 
ACTGAGAGCT TCTTGGATTC GGTTCGGACA GTTCCAACTC TGGCTCGAGA CTCATCTCTT 
CATTCGGAGA TCATGGAGGC GGTGCTCTCA GGGGGACGGG ACTGTAGTGG AAGGGCAGGC 
GGCAGCGAAA GACAGTCGGA TGGTACAGTC CTGTTTGAGG ACAGCGCCGC CGCTGACCTA 
TCGCTCTGCT CTGCCCACCT GCTGCCGGAT TCCTTGCCCC CCTGGGACCT GGACCCCTCA 
CCGCCACCAT CAGCTGGTGT ACTAGAGCGG TCCAAAAGTA GTCGCTTCCT GTCTACAGCA 
GGGCCTCTGT TTCTGGACGA GCCTCATTTG GTTAAGTCAC ACAGTCTGAT GTTCACATCA 
CGTGGGTACT ATGGTGGCAT GGGTGTGCAG GTGAAGGCGG CCGAATACAC CAGCATCACA 
GATTGCATCG ACACCCGTTG CGTCTCAACC CCTTACCCTG TGCCAGAGCG GTCAGACTCA 
CCTGGAGGAT CTTTCACCTT CGATAAGCCT CAAGACCTTG GCGTCTCGCA CCCTGAGAGA 
GACGCTGAGC TCAGCCACGC TGAGTCTGAC TTAGAGGAGC CAGCAGAGGG TTCGGGAGAC 
ACATGTAAGG TGGGTCAGAG TAGCAGTAGT GGGGTAATAG GTGCAGACCT GGGCCTGGGC 
CTCGCACTAG GCCCGTTTTG CTCACCCATC TCTAGACTAG AGCGAGCAAA CAGCTGCTCC 
TCTTCAGAGG AGTCGCACTC CAACATCTAC TCTCGAAAGA GCTTCTCAAT AAGCGAGAGA 
ATGGACAAGG GCCGGGGGAG CTCCAGGAAC CCTTTCCAGA AGGCCAGAAC CGGGGCGAGG 
CTGGAGGGTA AGACAGACTC ACTGTCTATG AGGAAAATGG CCAAACCGTC AGCTTTCAGA 
AGTTTTGACA GCAGACACAA CTACACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332145.1
CDS	397..5706
Translation	MWGPFPDGGNVRPGAMNQLDAPRPLNWTIRKLCHAAFLPSVRLLKAQKSWIERAFSKRECVHIIVSTKDHHRCCCGRLIGQHVGLPPSISSNQNEKSERVPKNDSLSEKWSISKHTQLSPTDAFGTIEFQGGGHSNKAMYVRVSYDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLVGKDVVRPYQTMSNPLSKLTVLNSLHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGIINESLRDQLLVTIQKTFTYSRTQAQHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQSMLDALVLDRVDFVKLLIENGVSMHRFLTLSRLEELYNTRHGPSNTLYHLVRDVKKQEYPGFSWIYLKGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDMPIRRGRQKTTRKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNQNSREFGQLAVELLDQSYKQDEQMAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGLLLPPSILSLEFKNKDEMSYMPQDQDTYLQEKDVDEPEKQAKEKEEEDMEFTVRSYCETQYNSVAMLGNVSSEASRKKQVEEVQNRHRLIPLGRKIYEFYNAPIVKFWFHTLAYVGYLMLFNYIVLVKMDLWPSPQEWIVIAYIFTNGIEKMREILMSEPGKLLQKVKVWLQEYWNITDLMAILIFSIGMVLRLQDPPLMSYGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDHVNSRKLHPKVNDKLWLVENYMRTQGTGRYSGPSHIAPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHITMVLKHLCCRWRKHDEDERDYGLKLFITEDELKKVHDFEEQCMEEYFREKDDRFNSSNDERIRVTSERVENMAMRLEEVNEREHFMKASLQTVDIRLAQMEEMIGRIAVALERVAGMDRGEVNKARSRTSSDCTDTNYILRQSSFNSQEGNSYRLQESLEQGGDESISPTSPTALAPRVRSHSFYVSHSSKDRSGADRGEGFFKDKLFSLHRANSSQSVSSGAGPKESKPTPLNTLSVQQQLRPSSCIDIYVSASEDVPPTESFLDSVRTVPTLARDSSLHSEIMEAVLSGGRDCSGRAGGSERQSDGTVLFEDSAAADLSLCSAHLLPDSLPPWDLDPSPPPSAGVLERSKSSRFLSTAGPLFLDEPHLVKSHSLMFTSRGYYGGMGVQVKAAEYTSITDCIDTRCVSTPYPVPERSDSPGGSFTFDKPQDLGVSHPERDAELSHAESDLEEPAEGSGDTCKVGQSSSSGVIGADLGLGLALGPFCSPISRLERANSCSSSEESHSNIYSRKSFSISERMDKGRGSSRNPFQKARTGARLEGKTDSLSMRKMAKPSAFRSFDSRHNYT

Target ORF information:

RefSeq Version	XM_021476470.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476470.1

ORF Insert Sequence:

ATGTGGGGCC CTTTTCCGGA TGGCGGGAAC GTCCGGCCGG GAGCGATGAA CCAGCTGGAT 
GCTCCTCGGC CACTCAACTG GACCATACGG AAACTGTGCC ATGCAGCCTT CCTCCCGTCT 
GTGCGCCTGC TTAAGGCACA GAAATCATGG ATAGAAAGAG CGTTCAGCAA GAGAGAATGT 
GTGCACATTA TTGTAAGCAC CAAAGACCAC CATAGGTGCT GTTGTGGCCG TCTAATTGGT 
CAACATGTTG GCCTGCCCCC CAGCATCTCA TCCAATCAGA ATGAGAAGTC AGAACGCGTG 
CCAAAGAATG ACAGCTTGTC AGAGAAGTGG TCCATCAGCA AGCACACCCA GCTCAGTCCC 
ACCGACGCTT TTGGGACCAT CGAGTTCCAG GGAGGAGGAC ACTCTAATAA AGCAATGTAT 
GTACGAGTGT CTTACGACAC AAAACCAGAT TTACTGCTGC ATCTGATGAC AAAGGAGTGG 
CAACTGGAGC TTCCCAAGCT GCTCATCTCT GTTCACGGAG GACTTCAAAA CTTTGAGTTG 
CAGCCCAAAC TAAAGCAAGT CTTCGGCAAA GGTCTTATAA AGGCTGCAAT GACTACCGGA 
GCCTGGATCT TCACTGGAGG AGTTAATACT GGAGTCATCA GACATGTTGG TGATGCTCTG 
AAAGATCATG CTTCCAAATC AAGAGGGAAG ATCTGCACTA TCGGCATTGC CCCCTGGGGG 
ATTGTGGAGA ACCAGGAAGA CCTGGTGGGC AAAGATGTGG TCCGGCCATA CCAGACGATG 
TCTAACCCTC TTAGCAAGCT AACAGTACTC AACAGCCTAC ACTCACACTT CATTTTGGCT 
GATAATGGCA CCACCGGAAA GTACGGAGCA GAAGTCAAAC TGCGCAGGCA GCTGGAGAAG 
CACATCTCTC TGCAAAAGAT TAACACACGG ATTGGACAGG GTGTTCCAGT AGTGGCTCTG 
ATAGTGGAGG GGGGTCCGAA TGTCATCTCT ATCGTCCTGG AGTACCTAAG AGACACCCCT 
CCGGTGCCTG TTGTGGTGTG TGACGGCAGC GGTCGAGCCT CTGACATCCT GGCCTTTGGG 
CACAAGTACT CAGAGGAGGG CGGAATCATA AACGAATCTT TGAGAGACCA GTTATTAGTC 
ACCATTCAGA AGACGTTCAC ATACAGCCGG ACGCAGGCCC AGCATCTCTT CATCATTCTG 
ATGGAGTGCA TGAAGAAGAA AGAACTGATC ACAGTGTTTC GGATGGGATC AGAGGGACAC 
CAGGACATCG ATCTAGCCAT TCTAACAGCT CTTCTGAAAG GAGCGAATGC ATCAGCTCCA 
GATCAGCTAA GTCTGGCTTT GGCCTGGAAC AGAGTTGACA TCGCCCGGAG CCAGATCTTC 
ATTTACGGAC AGCAGTGGCC TGTTGGCTCT CTGGAGCAGT CTATGCTGGA CGCGCTGGTG 
CTGGACCGAG TGGACTTTGT AAAGCTGTTG ATTGAGAACG GAGTGAGCAT GCACCGTTTC 
CTGACACTCT CCAGACTTGA AGAGCTTTAT AATACGAGGC ACGGACCATC CAACACCTTG 
TACCACCTTG TCAGAGATGT GAAAAAGCAA GAATATCCAG GGTTCAGTTG GATCTACCTC 
AAGGGTAACC TGCCCCCCGA CTACCGCATT AGCCTGATTG ACATCGGATT GGTTATTGAA 
TACCTCATGG GTGGAGCCTA TCGCTGCAAT TACACCAGGA AGAGATTTCG GACCCTCTAT 
CACAACCTCT TTGGACCCAA AAGACCAAAA GCCCTGAAGC TGCTTGGAAT GGAGGATGAC 
ATGCCCATTA GACGAGGGCG TCAGAAGACT ACGCGGAAGC GAGAGGAGGA AGTAGATATT 
GACCTGGATG ACCCAGAAAT CAATCATTTC CCTTTCCCAT TCCATGAGCT GATGGTTTGG 
GCTGTGCTGA TGAAACGGCA GAAGATGGCA CTGTTCTTCT GGCAGCATGG AGAGGAGGCC 
ATGGCTAAAG CGCTGGTGGC CTGCAAGCTC TGTAAGGCAA TGGCTCACGA AGCTTCTGAG 
AACGACATGG TCGATGACAT TTCACAGGAG CTCAATCAAA ACTCAAGGGA GTTTGGTCAG 
CTGGCTGTTG AGCTGCTCGA TCAGTCCTAT AAGCAGGATG AACAGATGGC TATGAAGCTG 
TTGACCTACG AGCTGAAGAA CTGGAGTAAT GCCACCTGTC TGCAGCTGGC AGTGGCTGCC 
AAGCACCGAG ACTTCATCGC CCACACCTGT AGCCAGATGC TGCTCACAGA CATGTGGATG 
GGACGTCTGC GCATGCGCAA GAACTCTGGC CTGAAGGTTA TCTTGGGCCT GCTTCTCCCC 
CCTTCCATAT TGAGTCTGGA GTTTAAAAAT AAAGACGAGA TGTCATACAT GCCTCAGGAT 
CAGGACACAT ATCTGCAGGA GAAGGATGTG GATGAGCCCG AGAAACAAGC TAAGGAGAAA 
GAGGAGGAGG ACATGGAGTT CACAGTAAGA TCTTACTGTG AGACGCAGTA CAACTCCGTG 
GCTATGTTGG GAAACGTCAG TTCAGAAGCA TCGCGCAAGA AACAGGTGGA GGAGGTTCAG 
AACCGGCATC GGCTCATTCC ATTGGGTCGC AAAATATATG AGTTTTACAA CGCCCCTATC 
GTCAAGTTCT GGTTTCACAC GCTGGCGTAT GTAGGATACT TGATGCTCTT CAACTATATT 
GTGCTGGTGA AGATGGACCT GTGGCCTTCT CCTCAGGAGT GGATTGTTAT TGCCTACATA 
TTCACCAATG GCATCGAGAA GATGAGAGAG ATCCTGATGT CCGAACCTGG GAAGCTGCTT 
CAGAAGGTGA AAGTGTGGTT ACAGGAGTAC TGGAACATCA CTGACCTCAT GGCCATCCTC 
ATCTTCTCCA TCGGGATGGT GCTTCGTCTG CAGGACCCGC CGCTGATGAG CTACGGCAGG 
GTCATTTACT GCGTCAATAT CATTTATTGG TACATACGAC TCCTCGACAT CTTCGGAGTG 
AACAAATACC TGGGTCCATA CGTCATGATG ATCGGCAAGA TGATGATTGA CATGATGTAT 
TTTGTGATCA TCATGTTGGT GGTGCTGATG AGTTTCGGTG TGGCGCGTCA GGCCATCCTT 
AATCCCAATG AAGACCCATC CTGGATGCTG GCACGCAATA TCTTCTTCAT GCCATACTGG 
ATGATCTACG GAGAGGTGTT TGCTGACCAA ATCGACCATG TGAATAGTAG GAAGCTACAT 
CCCAAAGTGA ATGACAAACT ATGGCTGGTC GAAAATTACA TGCGCACTCA AGGAACTGGG 
CGTTACAGTG GTCCATCCCA TATAGCTCCT TGTGGTCAGA ACATCACCAC AGAAGAAGGA 
GTTGTCATGC CTCTTCCACC CTGCAAAACT GGAGCTTGGA TTGTCCCTGC CATAATGGCC 
TGCTACCTGC TGGTGGCCAA CATCCTGCTA GTTAACTTGC TCATTGCTGT CTTTAATAAC 
ACTTTCTTTG AAGTCAAGTC CATTTCCAAC CAGGTGTGGA AGTTTCAGAG GTATCAGCTG 
ATCATGACCT TCCATGAACG CCCGGTCCTT CCTCCTCCAC TCATCATCTT CAGCCACATA 
ACTATGGTTC TCAAACACCT GTGCTGTCGC TGGAGGAAGC ACGATGAGGA TGAACGGGAC 
TATGGGCTGA AGCTTTTCAT CACTGAAGAT GAACTGAAGA AAGTGCATGA CTTTGAAGAG 
CAGTGCATGG AGGAGTATTT CAGAGAGAAA GATGACCGCT TCAATTCCTC CAATGACGAA 
AGGATACGTG TGACATCTGA AAGGGTGGAG AACATGGCGA TGCGATTAGA GGAGGTGAAT 
GAGAGGGAAC ATTTTATGAA AGCGTCCTTG CAGACTGTAG ACATCCGGCT CGCCCAGATG 
GAGGAGATGA TCGGCCGCAT TGCTGTGGCC CTAGAGAGAG TTGCTGGAAT GGACAGAGGC 
GAAGTTAACA AAGCCCGGTC CAGGACATCA TCAGACTGCA CTGATACCAA TTACATCCTG 
CGCCAAAGCA GTTTCAACAG CCAAGAAGGC AATTCCTACA GGCTACAGGA GTCTCTGGAG 
CAGGGGGGCG ACGAGTCCAT TTCTCCTACT TCCCCAACCG CCCTGGCTCC CCGTGTCCGC 
AGTCACTCCT TCTACGTGAG CCACTCTTCC AAAGACAGGA GTGGGGCGGA CCGTGGAGAG 
GGCTTCTTTA AAGACAAGTT GTTCAGCCTC CACCGGGCCA ACAGCTCACA GTCTGTGTCC 
TCAGGAGCAG GTCCCAAAGA GTCCAAGCCA ACCCCTTTGA ATACTTTATC CGTGCAGCAG 
CAGCTCCGCC CTTCTTCCTG CATAGACATC TATGTTTCTG CCTCAGAGGA CGTCCCGCCC 
ACTGAGAGCT TCTTGGATTC GGTTCGGACA GTTCCAACTC TGGCTCGAGA CTCATCTCTT 
CATTCGGAGA TCATGGAGGC GGTGCTCTCA GGGGGACGGG ACTGTAGTGG AAGGGCAGGC 
GGCAGCGAAA GACAGTCGGA TGGTACAGTC CTGTTTGAGG ACAGCGCCGC CGCTGACCTA 
TCGCTCTGCT CTGCCCACCT GCTGCCGGAT TCCTTGCCCC CCTGGGACCT GGACCCCTCA 
CCGCCACCAT CAGCTGGTGT ACTAGAGCGG TCCAAAAGTA GTCGCTTCCT GTCTACAGCA 
GGGCCTCTGT TTCTGGACGA GCCTCATTTG GTTAAGTCAC ACAGTCTGAT GTTCACATCA 
CGTGGGTACT ATGGTGGCAT GGGTGTGCAG GTGAAGGCGG CCGAATACAC CAGCATCACA 
GATTGCATCG ACACCCGTTG CGTCTCAACC CCTTACCCTG TGCCAGAGCG GTCAGACTCA 
CCTGGAGGAT CTTTCACCTT CGATAAGCCT CAAGACCTTG GCGTCTCGCA CCCTGAGAGA 
GACGCTGAGC TCAGCCACGC TGAGTCTGAC TTAGAGGAGC CAGCAGAGGG TTCGGGAGAC 
ACATGTAAGG TGGGTCAGAG TAGCAGTAGT GGGGTAATAG GTGCAGACCT GGGCCTGGGC 
CTCGCACTAG GCCCGTTTTG CTCACCCATC TCTAGACTAG AGCGAGCAAA CAGCTGCTCC 
TCTTCAGAGG AGTCGCACTC CAACATCTAC TCTCGAAAGA GCTTCTCAAT AAGCGAGAGA 
ATGGACAAGG GCCGGGGGAG CTCCAGGAAC CCTTTCCAGA AGGCCAGAAC CGGGGCGAGG 
CTGGAGGGTA AGACAGACTC ACTGTCTATG AGGAAAATGG CCAAACCGTC AGCTTTCAGA 
AGTTTTGACA GCAGACACAA CTACACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm3 ( XM_021476471.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332146.1 Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X5, mRNA.

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CloneID	ODa142790
Clone ID Related Accession (Same CDS sequence)	XM_021476471.1
Accession Version	XM_021476471.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily M member 3 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAAAA GAAAATGGGA GGGGAGGAGA GAAGGCTTCA AGGTGAGCTC CACGAGGGAG 
GAGGCACAGG GGCCTGGACG CCCCTCCACC AGAGGGCGCT TCTCCGAATC TTGGAAGAGA 
CTGAGCTCCA GACAGGGGTC TACCAAGAGG TCAGGACTGG CCTCCCAGCA AGCTGCGGCA 
CAGAAATCAT GGATAGAAAG AGCGTTCAGC AAGAGAGAAT GTGTGCACAT TATTGTAAGC 
ACCAAAGACC ACCATAGGTG CTGTTGTGGC CGTCTAATTG GTCAACATGT TGGCCTGCCC 
CCCAGCATCT CATCCAATCA GAATGAGAAG TCAGAACGCG TGCCAAAGAA TGACAGCTTG 
TCAGAGAAGT GGTCCATCAG CAAGCACACC CAGCTCAGTC CCACCGACGC TTTTGGGACC 
ATCGAGTTCC AGGGAGGAGG ACACTCTAAT AAAGCAATGT ATGTACGAGT GTCTTACGAC 
ACAAAACCAG ATTTACTGCT GCATCTGATG ACAAAGGAGT GGCAACTGGA GCTTCCCAAG 
CTGCTCATCT CTGTTCACGG AGGACTTCAA AACTTTGAGT TGCAGCCCAA ACTAAAGCAA 
GTCTTCGGCA AAGGTCTTAT AAAGGCTGCA ATGACTACCG GAGCCTGGAT CTTCACTGGA 
GGAGTTAATA CTGGAGTCAT CAGACATGTT GGTGATGCTC TGAAAGATCA TGCTTCCAAA 
TCAAGAGGGA AGATCTGCAC TATCGGCATT GCCCCCTGGG GGATTGTGGA GAACCAGGAA 
GACCTGGTGG GCAAAGATGT GGTCCGGCCA TACCAGACGA TGTCTAACCC TCTTAGCAAG 
CTAACAGTAC TCAACAGCCT ACACTCACAC TTCATTTTGG CTGATAATGG CACCACCGGA 
AAGTACGGAG CAGAAGTCAA ACTGCGCAGG CAGCTGGAGA AGCACATCTC TCTGCAAAAG 
ATTAACACAC GGATTGGACA GGGTGTTCCA GTAGTGGCTC TGATAGTGGA GGGGGGTCCG 
AATGTCATCT CTATCGTCCT GGAGTACCTA AGAGACACCC CTCCGGTGCC TGTTGTGGTG 
TGTGACGGCA GCGGTCGAGC CTCTGACATC CTGGCCTTTG GGCACAAGTA CTCAGAGGAG 
GGCGGAATCA TAAACGAATC TTTGAGAGAC CAGTTATTAG TCACCATTCA GAAGACGTTC 
ACATACAGCC GGACGCAGGC CCAGCATCTC TTCATCATTC TGATGGAGTG CATGAAGAAG 
AAAGAACTGA TCACAGTGTT TCGGATGGGA TCAGAGGGAC ACCAGGACAT CGATCTAGCC 
ATTCTAACAG CTCTTCTGAA AGGAGCGAAT GCATCAGCTC CAGATCAGCT AAGTCTGGCT 
TTGGCCTGGA ACAGAGTTGA CATCGCCCGG AGCCAGATCT TCATTTACGG ACAGCAGTGG 
CCTGTTGGCT CTCTGGAGCA GTCTATGCTG GACGCGCTGG TGCTGGACCG AGTGGACTTT 
GTAAAGCTGT TGATTGAGAA CGGAGTGAGC ATGCACCGTT TCCTGACACT CTCCAGACTT 
GAAGAGCTTT ATAATACGAG GCACGGACCA TCCAACACCT TGTACCACCT TGTCAGAGAT 
GTGAAAAAGG GTAACCTGCC CCCCGACTAC CGCATTAGCC TGATTGACAT CGGATTGGTT 
ATTGAATACC TCATGGGTGG AGCCTATCGC TGCAATTACA CCAGGAAGAG ATTTCGGACC 
CTCTATCACA ACCTCTTTGG ACCCAAAAGA CCAAAAGCCC TGAAGCTGCT TGGAATGGAG 
GATGACATGC CCATTAGACG AGGGCGTCAG AAGACTACGC GGAAGCGAGA GGAGGAAGTA 
GATATTGACC TGGATGACCC AGAAATCAAT CATTTCCCTT TCCCATTCCA TGAGCTGATG 
GTTTGGGCTG TGCTGATGAA ACGGCAGAAG ATGGCACTGT TCTTCTGGCA GCATGGAGAG 
GAGGCCATGG CTAAAGCGCT GGTGGCCTGC AAGCTCTGTA AGGCAATGGC TCACGAAGCT 
TCTGAGAACG ACATGGTCGA TGACATTTCA CAGGAGCTCA ATCAAAACTC AAGGGAGTTT 
GGTCAGCTGG CTGTTGAGCT GCTCGATCAG TCCTATAAGC AGGATGAACA GATGGCTATG 
AAGCTGTTGA CCTACGAGCT GAAGAACTGG AGTAATGCCA CCTGTCTGCA GCTGGCAGTG 
GCTGCCAAGC ACCGAGACTT CATCGCCCAC ACCTGTAGCC AGATGCTGCT CACAGACATG 
TGGATGGGAC GTCTGCGCAT GCGCAAGAAC TCTGGCCTGA AGGTTATCTT GGGCCTGCTT 
CTCCCCCCTT CCATATTGAG TCTGGAGTTT AAAAATAAAG ACGAGATGTC ATACATGCCT 
CAGGATCAGG ACACATATCT GCAGGAGAAG GATGTGGATG AGCCCGAGAA ACAAGCTAAG 
GAGAAAGAGG AGGAGGACAT GGAGTTCACA GCTATGTTGG GAAACGTCAG TTCAGAAGCA 
TCGCGCAAGA AACAGGTGGA GGAGGTTCAG AACCGGCATC GGCTCATTCC ATTGGGTCGC 
AAAATATATG AGTTTTACAA CGCCCCTATC GTCAAGTTCT GGTTTCACAC GCTGGCGTAT 
GTAGGATACT TGATGCTCTT CAACTATATT GTGCTGGTGA AGATGGACCT GTGGCCTTCT 
CCTCAGGAGT GGATTGTTAT TGCCTACATA TTCACCAATG GCATCGAGAA GATGAGAGAG 
ATCCTGATGT CCGAACCTGG GAAGCTGCTT CAGAAGGTGA AAGTGTGGTT ACAGGAGTAC 
TGGAACATCA CTGACCTCAT GGCCATCCTC ATCTTCTCCA TCGGGATGGT GCTTCGTCTG 
CAGGACCCGC CGCTGATGAG CTACGGCAGG GTCATTTACT GCGTCAATAT CATTTATTGG 
TACATACGAC TCCTCGACAT CTTCGGAGTG AACAAATACC TGGGTCCATA CGTCATGATG 
ATCGGCAAGA TGATGATTGA CATGATGTAT TTTGTGATCA TCATGTTGGT GGTGCTGATG 
AGTTTCGGTG TGGCGCGTCA GGCCATCCTT AATCCCAATG AAGACCCATC CTGGATGCTG 
GCACGCAATA TCTTCTTCAT GCCATACTGG ATGATCTACG GAGAGGTGTT TGCTGACCAA 
ATCGACCATG TGAATAGTAG GAAGCTACAT CCCAAAGTGA ATGACAAACT ATGGCTGGTC 
GAAAATTACA TGCGCACTCA AGGAACTGGG CGTTACAGTG GTCCATCCCA TATAGCTCCT 
TGTGGTCAGA ACATCACCAC AGAAGAAGGA GTTGTCATGC CTCTTCCACC CTGCAAAACT 
GGAGCTTGGA TTGTCCCTGC CATAATGGCC TGCTACCTGC TGGTGGCCAA CATCCTGCTA 
GTTAACTTGC TCATTGCTGT CTTTAATAAC ACTTTCTTTG AAGTCAAGTC CATTTCCAAC 
CAGGTGTGGA AGTTTCAGAG GTATCAGCTG ATCATGACCT TCCATGAACG CCCGGTCCTT 
CCTCCTCCAC TCATCATCTT CAGCCACATA ACTATGGTTC TCAAACACCT GTGCTGTCGC 
TGGAGGAAGC ACGATGAGGA TGAACGGGAC TATGGGCTGA AGCTTTTCAT CACTGAAGAT 
GAACTGAAGA AAGTGCATGA CTTTGAAGAG CAGTGCATGG AGGAGTATTT CAGAGAGAAA 
GATGACCGCT TCAATTCCTC CAATGACGAA AGGATACGTG TGACATCTGA AAGGGTGGAG 
AACATGGCGA TGCGATTAGA GGAGGTGAAT GAGAGGGAAC ATTTTATGAA AGCGTCCTTG 
CAGACTGTAG ACATCCGGCT CGCCCAGATG GAGGAGATGA TCGGCCGCAT TGCTGTGGCC 
CTAGAGAGAG TTGCTGGAAT GGACAGAGGC GAAGTTAACA AAGCCCGGTC CAGGACATCA 
TCAGACTGCA CTGATACCAA TTACATCCTG CGCCAAAGCA GTTTCAACAG CCAAGAAGGC 
AATTCCTACA GGCTACAGGA GTCTCTGGAG CAGGGGGGCG ACGAGTCCAT TTCTCCTACT 
TCCCCAACCG CCCTGGCTCC CCGTGTCCGC AGTCACTCCT TCTACGTGAG CCACTCTTCC 
AAAGACAGGA GTGGGGCGGA CCGTGGAGAG GGCTTCTTTA AAGACAAGTT GTTCAGCCTC 
CACCGGGCCA ACAGCTCACA GTCTGTGTCC TCAGGAGCAG GTCCCAAAGA GTCCAAGCCA 
ACCCCTTTGA ATACTTTATC CGTGCAGCAG CAGCTCCGCC CTTCTTCCTG CATAGACATC 
TATGTTTCTG CCTCAGAGGA CGTCCCGCCC ACTGAGAGCT TCTTGGATTC GGTTCGGACA 
GTTCCAACTC TGGCTCGAGA CTCATCTCTT CATTCGGAGA TCATGGAGGC GGTGCTCTCA 
GGGGGACGGG ACTGTAGTGG AAGGGCAGGC GGCAGCGAAA GACAGTCGGA TGGTACAGTC 
CTGTTTGAGG ACAGCGCCGC CGCTGACCTA TCGCTCTGCT CTGCCCACCT GCTGCCGGAT 
TCCTTGCCCC CCTGGGACCT GGACCCCTCA CCGCCACCAT CAGCTGGTGT ACTAGAGCGG 
TCCAAAAGTA GTCGCTTCCT GTCTACAGCA GGGCCTCTGT TTCTGGACGA GCCTCATTTG 
GTTAAGTCAC ACAGTCTGAT GTTCACATCA CGTGGGTACT ATGGTGGCAT GGGTGTGCAG 
GTGAAGGCGG CCGAATACAC CAGCATCACA GATTGCATCG ACACCCGTTG CGTCTCAACC 
CCTTACCCTG TGCCAGAGCG GTCAGACTCA CCTGGAGGAT CTTTCACCTT CGATAAGCCT 
CAAGACCTTG GCGTCTCGCA CCCTGAGAGA GACGCTGAGC TCAGCCACGC TGAGTCTGAC 
TTAGAGGAGC CAGCAGAGGG TTCGGGAGAC ACATGTAAGG TGGGTCAGAG TAGCAGTAGT 
GGGGTAATAG GTGCAGACCT GGGCCTGGGC CTCGCACTAG GCCCGTTTTG CTCACCCATC 
TCTAGACTAG AGCGAGCAAA CAGCTGCTCC TCTTCAGAGG AGTCGCACTC CAACATCTAC 
TCTCGAAAGA GCTTCTCAAT AAGCGAGAGA ATGGACAAGG GCCGGGGGAG CTCCAGGAAC 
CCTTTCCAGA AGGCCAGAAC CGGGGCGAGG CTGGAGGGTA AGACAGACTC ACTGTCTATG 
AGGAAAATGG CCAAACCGTC AGCTTTCAGA AGTTTTGACA GCAGACACAA CTACACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332146.1
CDS	378..5657
Translation	MTKRKWEGRREGFKVSSTREEAQGPGRPSTRGRFSESWKRLSSRQGSTKRSGLASQQAAAQKSWIERAFSKRECVHIIVSTKDHHRCCCGRLIGQHVGLPPSISSNQNEKSERVPKNDSLSEKWSISKHTQLSPTDAFGTIEFQGGGHSNKAMYVRVSYDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLVGKDVVRPYQTMSNPLSKLTVLNSLHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGIINESLRDQLLVTIQKTFTYSRTQAQHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQSMLDALVLDRVDFVKLLIENGVSMHRFLTLSRLEELYNTRHGPSNTLYHLVRDVKKGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDMPIRRGRQKTTRKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNQNSREFGQLAVELLDQSYKQDEQMAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGLLLPPSILSLEFKNKDEMSYMPQDQDTYLQEKDVDEPEKQAKEKEEEDMEFTAMLGNVSSEASRKKQVEEVQNRHRLIPLGRKIYEFYNAPIVKFWFHTLAYVGYLMLFNYIVLVKMDLWPSPQEWIVIAYIFTNGIEKMREILMSEPGKLLQKVKVWLQEYWNITDLMAILIFSIGMVLRLQDPPLMSYGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDHVNSRKLHPKVNDKLWLVENYMRTQGTGRYSGPSHIAPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHITMVLKHLCCRWRKHDEDERDYGLKLFITEDELKKVHDFEEQCMEEYFREKDDRFNSSNDERIRVTSERVENMAMRLEEVNEREHFMKASLQTVDIRLAQMEEMIGRIAVALERVAGMDRGEVNKARSRTSSDCTDTNYILRQSSFNSQEGNSYRLQESLEQGGDESISPTSPTALAPRVRSHSFYVSHSSKDRSGADRGEGFFKDKLFSLHRANSSQSVSSGAGPKESKPTPLNTLSVQQQLRPSSCIDIYVSASEDVPPTESFLDSVRTVPTLARDSSLHSEIMEAVLSGGRDCSGRAGGSERQSDGTVLFEDSAAADLSLCSAHLLPDSLPPWDLDPSPPPSAGVLERSKSSRFLSTAGPLFLDEPHLVKSHSLMFTSRGYYGGMGVQVKAAEYTSITDCIDTRCVSTPYPVPERSDSPGGSFTFDKPQDLGVSHPERDAELSHAESDLEEPAEGSGDTCKVGQSSSSGVIGADLGLGLALGPFCSPISRLERANSCSSSEESHSNIYSRKSFSISERMDKGRGSSRNPFQKARTGARLEGKTDSLSMRKMAKPSAFRSFDSRHNYT

Target ORF information:

RefSeq Version	XM_021476471.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476471.1

ORF Insert Sequence:

ATGACAAAAA GAAAATGGGA GGGGAGGAGA GAAGGCTTCA AGGTGAGCTC CACGAGGGAG 
GAGGCACAGG GGCCTGGACG CCCCTCCACC AGAGGGCGCT TCTCCGAATC TTGGAAGAGA 
CTGAGCTCCA GACAGGGGTC TACCAAGAGG TCAGGACTGG CCTCCCAGCA AGCTGCGGCA 
CAGAAATCAT GGATAGAAAG AGCGTTCAGC AAGAGAGAAT GTGTGCACAT TATTGTAAGC 
ACCAAAGACC ACCATAGGTG CTGTTGTGGC CGTCTAATTG GTCAACATGT TGGCCTGCCC 
CCCAGCATCT CATCCAATCA GAATGAGAAG TCAGAACGCG TGCCAAAGAA TGACAGCTTG 
TCAGAGAAGT GGTCCATCAG CAAGCACACC CAGCTCAGTC CCACCGACGC TTTTGGGACC 
ATCGAGTTCC AGGGAGGAGG ACACTCTAAT AAAGCAATGT ATGTACGAGT GTCTTACGAC 
ACAAAACCAG ATTTACTGCT GCATCTGATG ACAAAGGAGT GGCAACTGGA GCTTCCCAAG 
CTGCTCATCT CTGTTCACGG AGGACTTCAA AACTTTGAGT TGCAGCCCAA ACTAAAGCAA 
GTCTTCGGCA AAGGTCTTAT AAAGGCTGCA ATGACTACCG GAGCCTGGAT CTTCACTGGA 
GGAGTTAATA CTGGAGTCAT CAGACATGTT GGTGATGCTC TGAAAGATCA TGCTTCCAAA 
TCAAGAGGGA AGATCTGCAC TATCGGCATT GCCCCCTGGG GGATTGTGGA GAACCAGGAA 
GACCTGGTGG GCAAAGATGT GGTCCGGCCA TACCAGACGA TGTCTAACCC TCTTAGCAAG 
CTAACAGTAC TCAACAGCCT ACACTCACAC TTCATTTTGG CTGATAATGG CACCACCGGA 
AAGTACGGAG CAGAAGTCAA ACTGCGCAGG CAGCTGGAGA AGCACATCTC TCTGCAAAAG 
ATTAACACAC GGATTGGACA GGGTGTTCCA GTAGTGGCTC TGATAGTGGA GGGGGGTCCG 
AATGTCATCT CTATCGTCCT GGAGTACCTA AGAGACACCC CTCCGGTGCC TGTTGTGGTG 
TGTGACGGCA GCGGTCGAGC CTCTGACATC CTGGCCTTTG GGCACAAGTA CTCAGAGGAG 
GGCGGAATCA TAAACGAATC TTTGAGAGAC CAGTTATTAG TCACCATTCA GAAGACGTTC 
ACATACAGCC GGACGCAGGC CCAGCATCTC TTCATCATTC TGATGGAGTG CATGAAGAAG 
AAAGAACTGA TCACAGTGTT TCGGATGGGA TCAGAGGGAC ACCAGGACAT CGATCTAGCC 
ATTCTAACAG CTCTTCTGAA AGGAGCGAAT GCATCAGCTC CAGATCAGCT AAGTCTGGCT 
TTGGCCTGGA ACAGAGTTGA CATCGCCCGG AGCCAGATCT TCATTTACGG ACAGCAGTGG 
CCTGTTGGCT CTCTGGAGCA GTCTATGCTG GACGCGCTGG TGCTGGACCG AGTGGACTTT 
GTAAAGCTGT TGATTGAGAA CGGAGTGAGC ATGCACCGTT TCCTGACACT CTCCAGACTT 
GAAGAGCTTT ATAATACGAG GCACGGACCA TCCAACACCT TGTACCACCT TGTCAGAGAT 
GTGAAAAAGG GTAACCTGCC CCCCGACTAC CGCATTAGCC TGATTGACAT CGGATTGGTT 
ATTGAATACC TCATGGGTGG AGCCTATCGC TGCAATTACA CCAGGAAGAG ATTTCGGACC 
CTCTATCACA ACCTCTTTGG ACCCAAAAGA CCAAAAGCCC TGAAGCTGCT TGGAATGGAG 
GATGACATGC CCATTAGACG AGGGCGTCAG AAGACTACGC GGAAGCGAGA GGAGGAAGTA 
GATATTGACC TGGATGACCC AGAAATCAAT CATTTCCCTT TCCCATTCCA TGAGCTGATG 
GTTTGGGCTG TGCTGATGAA ACGGCAGAAG ATGGCACTGT TCTTCTGGCA GCATGGAGAG 
GAGGCCATGG CTAAAGCGCT GGTGGCCTGC AAGCTCTGTA AGGCAATGGC TCACGAAGCT 
TCTGAGAACG ACATGGTCGA TGACATTTCA CAGGAGCTCA ATCAAAACTC AAGGGAGTTT 
GGTCAGCTGG CTGTTGAGCT GCTCGATCAG TCCTATAAGC AGGATGAACA GATGGCTATG 
AAGCTGTTGA CCTACGAGCT GAAGAACTGG AGTAATGCCA CCTGTCTGCA GCTGGCAGTG 
GCTGCCAAGC ACCGAGACTT CATCGCCCAC ACCTGTAGCC AGATGCTGCT CACAGACATG 
TGGATGGGAC GTCTGCGCAT GCGCAAGAAC TCTGGCCTGA AGGTTATCTT GGGCCTGCTT 
CTCCCCCCTT CCATATTGAG TCTGGAGTTT AAAAATAAAG ACGAGATGTC ATACATGCCT 
CAGGATCAGG ACACATATCT GCAGGAGAAG GATGTGGATG AGCCCGAGAA ACAAGCTAAG 
GAGAAAGAGG AGGAGGACAT GGAGTTCACA GCTATGTTGG GAAACGTCAG TTCAGAAGCA 
TCGCGCAAGA AACAGGTGGA GGAGGTTCAG AACCGGCATC GGCTCATTCC ATTGGGTCGC 
AAAATATATG AGTTTTACAA CGCCCCTATC GTCAAGTTCT GGTTTCACAC GCTGGCGTAT 
GTAGGATACT TGATGCTCTT CAACTATATT GTGCTGGTGA AGATGGACCT GTGGCCTTCT 
CCTCAGGAGT GGATTGTTAT TGCCTACATA TTCACCAATG GCATCGAGAA GATGAGAGAG 
ATCCTGATGT CCGAACCTGG GAAGCTGCTT CAGAAGGTGA AAGTGTGGTT ACAGGAGTAC 
TGGAACATCA CTGACCTCAT GGCCATCCTC ATCTTCTCCA TCGGGATGGT GCTTCGTCTG 
CAGGACCCGC CGCTGATGAG CTACGGCAGG GTCATTTACT GCGTCAATAT CATTTATTGG 
TACATACGAC TCCTCGACAT CTTCGGAGTG AACAAATACC TGGGTCCATA CGTCATGATG 
ATCGGCAAGA TGATGATTGA CATGATGTAT TTTGTGATCA TCATGTTGGT GGTGCTGATG 
AGTTTCGGTG TGGCGCGTCA GGCCATCCTT AATCCCAATG AAGACCCATC CTGGATGCTG 
GCACGCAATA TCTTCTTCAT GCCATACTGG ATGATCTACG GAGAGGTGTT TGCTGACCAA 
ATCGACCATG TGAATAGTAG GAAGCTACAT CCCAAAGTGA ATGACAAACT ATGGCTGGTC 
GAAAATTACA TGCGCACTCA AGGAACTGGG CGTTACAGTG GTCCATCCCA TATAGCTCCT 
TGTGGTCAGA ACATCACCAC AGAAGAAGGA GTTGTCATGC CTCTTCCACC CTGCAAAACT 
GGAGCTTGGA TTGTCCCTGC CATAATGGCC TGCTACCTGC TGGTGGCCAA CATCCTGCTA 
GTTAACTTGC TCATTGCTGT CTTTAATAAC ACTTTCTTTG AAGTCAAGTC CATTTCCAAC 
CAGGTGTGGA AGTTTCAGAG GTATCAGCTG ATCATGACCT TCCATGAACG CCCGGTCCTT 
CCTCCTCCAC TCATCATCTT CAGCCACATA ACTATGGTTC TCAAACACCT GTGCTGTCGC 
TGGAGGAAGC ACGATGAGGA TGAACGGGAC TATGGGCTGA AGCTTTTCAT CACTGAAGAT 
GAACTGAAGA AAGTGCATGA CTTTGAAGAG CAGTGCATGG AGGAGTATTT CAGAGAGAAA 
GATGACCGCT TCAATTCCTC CAATGACGAA AGGATACGTG TGACATCTGA AAGGGTGGAG 
AACATGGCGA TGCGATTAGA GGAGGTGAAT GAGAGGGAAC ATTTTATGAA AGCGTCCTTG 
CAGACTGTAG ACATCCGGCT CGCCCAGATG GAGGAGATGA TCGGCCGCAT TGCTGTGGCC 
CTAGAGAGAG TTGCTGGAAT GGACAGAGGC GAAGTTAACA AAGCCCGGTC CAGGACATCA 
TCAGACTGCA CTGATACCAA TTACATCCTG CGCCAAAGCA GTTTCAACAG CCAAGAAGGC 
AATTCCTACA GGCTACAGGA GTCTCTGGAG CAGGGGGGCG ACGAGTCCAT TTCTCCTACT 
TCCCCAACCG CCCTGGCTCC CCGTGTCCGC AGTCACTCCT TCTACGTGAG CCACTCTTCC 
AAAGACAGGA GTGGGGCGGA CCGTGGAGAG GGCTTCTTTA AAGACAAGTT GTTCAGCCTC 
CACCGGGCCA ACAGCTCACA GTCTGTGTCC TCAGGAGCAG GTCCCAAAGA GTCCAAGCCA 
ACCCCTTTGA ATACTTTATC CGTGCAGCAG CAGCTCCGCC CTTCTTCCTG CATAGACATC 
TATGTTTCTG CCTCAGAGGA CGTCCCGCCC ACTGAGAGCT TCTTGGATTC GGTTCGGACA 
GTTCCAACTC TGGCTCGAGA CTCATCTCTT CATTCGGAGA TCATGGAGGC GGTGCTCTCA 
GGGGGACGGG ACTGTAGTGG AAGGGCAGGC GGCAGCGAAA GACAGTCGGA TGGTACAGTC 
CTGTTTGAGG ACAGCGCCGC CGCTGACCTA TCGCTCTGCT CTGCCCACCT GCTGCCGGAT 
TCCTTGCCCC CCTGGGACCT GGACCCCTCA CCGCCACCAT CAGCTGGTGT ACTAGAGCGG 
TCCAAAAGTA GTCGCTTCCT GTCTACAGCA GGGCCTCTGT TTCTGGACGA GCCTCATTTG 
GTTAAGTCAC ACAGTCTGAT GTTCACATCA CGTGGGTACT ATGGTGGCAT GGGTGTGCAG 
GTGAAGGCGG CCGAATACAC CAGCATCACA GATTGCATCG ACACCCGTTG CGTCTCAACC 
CCTTACCCTG TGCCAGAGCG GTCAGACTCA CCTGGAGGAT CTTTCACCTT CGATAAGCCT 
CAAGACCTTG GCGTCTCGCA CCCTGAGAGA GACGCTGAGC TCAGCCACGC TGAGTCTGAC 
TTAGAGGAGC CAGCAGAGGG TTCGGGAGAC ACATGTAAGG TGGGTCAGAG TAGCAGTAGT 
GGGGTAATAG GTGCAGACCT GGGCCTGGGC CTCGCACTAG GCCCGTTTTG CTCACCCATC 
TCTAGACTAG AGCGAGCAAA CAGCTGCTCC TCTTCAGAGG AGTCGCACTC CAACATCTAC 
TCTCGAAAGA GCTTCTCAAT AAGCGAGAGA ATGGACAAGG GCCGGGGGAG CTCCAGGAAC 
CCTTTCCAGA AGGCCAGAAC CGGGGCGAGG CTGGAGGGTA AGACAGACTC ACTGTCTATG 
AGGAAAATGG CCAAACCGTC AGCTTTCAGA AGTTTTGACA GCAGACACAA CTACACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm3 ( XM_021476472.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332147.1 Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X6, mRNA.

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CloneID	ODa142791
Clone ID Related Accession (Same CDS sequence)	XM_021476472.1
Accession Version	XM_021476472.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily M member 3 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGCC CTTTTCCGGA TGGCGGGAAC GTCCGGCCGG GAGCGATGAA CCAGCTGGAT 
GCTCCTCGGC CACTCAACTG GACCATACGG AAACTGTGCC ATGCAGCCTT CCTCCCGTCT 
GTGCGCCTGC TTAAGGCACA GAAATCATGG ATAGAAAGAG CGTTCAGCAA GAGAGAATGT 
GTGCACATTA TTGTAAGCAC CAAAGACCAC CATAGGTGCT GTTGTGGCCG TCTAATTGGT 
CAACATGTTG GCCTGCCCCC CAGCATCTCA TCCAATCAGA ATGAGAAGTC AGAACGCGTG 
CCAAAGAATG ACAGCTTGTC AGAGAAGTGG TCCATCAGCA AGCACACCCA GCTCAGTCCC 
ACCGACGCTT TTGGGACCAT CGAGTTCCAG GGAGGAGGAC ACTCTAATAA AGCAATGTAT 
GTACGAGTGT CTTACGACAC AAAACCAGAT TTACTGCTGC ATCTGATGAC AAAGGAGTGG 
CAACTGGAGC TTCCCAAGCT GCTCATCTCT GTTCACGGAG GACTTCAAAA CTTTGAGTTG 
CAGCCCAAAC TAAAGCAAGT CTTCGGCAAA GGTCTTATAA AGGCTGCAAT GACTACCGGA 
GCCTGGATCT TCACTGGAGG AGTTAATACT GGAGTCATCA GACATGTTGG TGATGCTCTG 
AAAGATCATG CTTCCAAATC AAGAGGGAAG ATCTGCACTA TCGGCATTGC CCCCTGGGGG 
ATTGTGGAGA ACCAGGAAGA CCTGGTGGGC AAAGATGTGG TCCGGCCATA CCAGACGATG 
TCTAACCCTC TTAGCAAGCT AACAGTACTC AACAGCCTAC ACTCACACTT CATTTTGGCT 
GATAATGGCA CCACCGGAAA GTACGGAGCA GAAGTCAAAC TGCGCAGGCA GCTGGAGAAG 
CACATCTCTC TGCAAAAGAT TAACACACGG ATTGGACAGG GTGTTCCAGT AGTGGCTCTG 
ATAGTGGAGG GGGGTCCGAA TGTCATCTCT ATCGTCCTGG AGTACCTAAG AGACACCCCT 
CCGGTGCCTG TTGTGGTGTG TGACGGCAGC GGTCGAGCCT CTGACATCCT GGCCTTTGGG 
CACAAGTACT CAGAGGAGGG CGGAATCATA AACGAATCTT TGAGAGACCA GTTATTAGTC 
ACCATTCAGA AGACGTTCAC ATACAGCCGG ACGCAGGCCC AGCATCTCTT CATCATTCTG 
ATGGAGTGCA TGAAGAAGAA AGAACTGATC ACAGTGTTTC GGATGGGATC AGAGGGACAC 
CAGGACATCG ATCTAGCCAT TCTAACAGCT CTTCTGAAAG GAGCGAATGC ATCAGCTCCA 
GATCAGCTAA GTCTGGCTTT GGCCTGGAAC AGAGTTGACA TCGCCCGGAG CCAGATCTTC 
ATTTACGGAC AGCAGTGGCC TGTTGGCTCT CTGGAGCAGT CTATGCTGGA CGCGCTGGTG 
CTGGACCGAG TGGACTTTGT AAAGCTGTTG ATTGAGAACG GAGTGAGCAT GCACCGTTTC 
CTGACACTCT CCAGACTTGA AGAGCTTTAT AATACGAGGC ACGGACCATC CAACACCTTG 
TACCACCTTG TCAGAGATGT GAAAAAGGGT AACCTGCCCC CCGACTACCG CATTAGCCTG 
ATTGACATCG GATTGGTTAT TGAATACCTC ATGGGTGGAG CCTATCGCTG CAATTACACC 
AGGAAGAGAT TTCGGACCCT CTATCACAAC CTCTTTGGAC CCAAAAGACC AAAAGCCCTG 
AAGCTGCTTG GAATGGAGGA TGACATGCCC ATTAGACGAG GGCGTCAGAA GACTACGCGG 
AAGCGAGAGG AGGAAGTAGA TATTGACCTG GATGACCCAG AAATCAATCA TTTCCCTTTC 
CCATTCCATG AGCTGATGGT TTGGGCTGTG CTGATGAAAC GGCAGAAGAT GGCACTGTTC 
TTCTGGCAGC ATGGAGAGGA GGCCATGGCT AAAGCGCTGG TGGCCTGCAA GCTCTGTAAG 
GCAATGGCTC ACGAAGCTTC TGAGAACGAC ATGGTCGATG ACATTTCACA GGAGCTCAAT 
CAAAACTCAA GGGAGTTTGG TCAGCTGGCT GTTGAGCTGC TCGATCAGTC CTATAAGCAG 
GATGAACAGA TGGCTATGAA GCTGTTGACC TACGAGCTGA AGAACTGGAG TAATGCCACC 
TGTCTGCAGC TGGCAGTGGC TGCCAAGCAC CGAGACTTCA TCGCCCACAC CTGTAGCCAG 
ATGCTGCTCA CAGACATGTG GATGGGACGT CTGCGCATGC GCAAGAACTC TGGCCTGAAG 
GTTATCTTGG GCCTGCTTCT CCCCCCTTCC ATATTGAGTC TGGAGTTTAA AAATAAAGAC 
GAGATGTCAT ACATGCCTCA GGATCAGGAC ACATATCTGC AGGAGAAGGA TGTGGATGAG 
CCCGAGAAAC AAGCTAAGGA GAAAGAGGAG GAGGACATGG AGTTCACAGT AAGATCTTAC 
TGTGAGACGC AGTACAACTC CGTGGCTATG TTGGGAAACG TCAGTTCAGA AGCATCGCGC 
AAGAAACAGG TGGAGGAGGT TCAGAACCGG CATCGGCTCA TTCCATTGGG TCGCAAAATA 
TATGAGTTTT ACAACGCCCC TATCGTCAAG TTCTGGTTTC ACACGCTGGC GTATGTAGGA 
TACTTGATGC TCTTCAACTA TATTGTGCTG GTGAAGATGG ACCTGTGGCC TTCTCCTCAG 
GAGTGGATTG TTATTGCCTA CATATTCACC AATGGCATCG AGAAGATGAG AGAGATCCTG 
ATGTCCGAAC CTGGGAAGCT GCTTCAGAAG GTGAAAGTGT GGTTACAGGA GTACTGGAAC 
ATCACTGACC TCATGGCCAT CCTCATCTTC TCCATCGGGA TGGTGCTTCG TCTGCAGGAC 
CCGCCGCTGA TGAGCTACGG CAGGGTCATT TACTGCGTCA ATATCATTTA TTGGTACATA 
CGACTCCTCG ACATCTTCGG AGTGAACAAA TACCTGGGTC CATACGTCAT GATGATCGGC 
AAGATGATGA TTGACATGAT GTATTTTGTG ATCATCATGT TGGTGGTGCT GATGAGTTTC 
GGTGTGGCGC GTCAGGCCAT CCTTAATCCC AATGAAGACC CATCCTGGAT GCTGGCACGC 
AATATCTTCT TCATGCCATA CTGGATGATC TACGGAGAGG TGTTTGCTGA CCAAATCGAC 
CATGTGAATA GTAGGAAGCT ACATCCCAAA GTGAATGACA AACTATGGCT GGTCGAAAAT 
TACATGCGCA CTCAAGGAAC TGGGCGTTAC AGTGGTCCAT CCCATATAGC TCCTTGTGGT 
CAGAACATCA CCACAGAAGA AGGAGTTGTC ATGCCTCTTC CACCCTGCAA AACTGGAGCT 
TGGATTGTCC CTGCCATAAT GGCCTGCTAC CTGCTGGTGG CCAACATCCT GCTAGTTAAC 
TTGCTCATTG CTGTCTTTAA TAACACTTTC TTTGAAGTCA AGTCCATTTC CAACCAGGTG 
TGGAAGTTTC AGAGGTATCA GCTGATCATG ACCTTCCATG AACGCCCGGT CCTTCCTCCT 
CCACTCATCA TCTTCAGCCA CATAACTATG GTTCTCAAAC ACCTGTGCTG TCGCTGGAGG 
AAGCACGATG AGGATGAACG GGACTATGGG CTGAAGCTTT TCATCACTGA AGATGAACTG 
AAGAAAGTGC ATGACTTTGA AGAGCAGTGC ATGGAGGAGT ATTTCAGAGA GAAAGATGAC 
CGCTTCAATT CCTCCAATGA CGAAAGGATA CGTGTGACAT CTGAAAGGGT GGAGAACATG 
GCGATGCGAT TAGAGGAGGT GAATGAGAGG GAACATTTTA TGAAAGCGTC CTTGCAGACT 
GTAGACATCC GGCTCGCCCA GATGGAGGAG ATGATCGGCC GCATTGCTGT GGCCCTAGAG 
AGAGTTGCTG GAATGGACAG AGGCGAAGTT AACAAAGCCC GGTCCAGGAC ATCATCAGAC 
TGCACTGATA CCAATTACAT CCTGCGCCAA AGCAGTTTCA ACAGCCAAGA AGGCAATTCC 
TACAGGCTAC AGGAGTCTCT GGAGCAGGGG GGCGACGAGT CCATTTCTCC TACTTCCCCA 
ACCGCCCTGG CTCCCCGTGT CCGCAGTCAC TCCTTCTACG TGAGCCACTC TTCCAAAGAC 
AGGAGTGGGG CGGACCGTGG AGAGGGCTTC TTTAAAGACA AGTTGTTCAG CCTCCACCGG 
GCCAACAGCT CACAGTCTGT GTCCTCAGGA GCAGGTCCCA AAGAGTCCAA GCCAACCCCT 
TTGAATACTT TATCCGTGCA GCAGCAGCTC CGCCCTTCTT CCTGCATAGA CATCTATGTT 
TCTGCCTCAG AGGACGTCCC GCCCACTGAG AGCTTCTTGG ATTCGGTTCG GACAGTTCCA 
ACTCTGGCTC GAGACTCATC TCTTCATTCG GAGATCATGG AGGCGGTGCT CTCAGGGGGA 
CGGGACTGTA GTGGAAGGGC AGGCGGCAGC GAAAGACAGT CGGATGGTAC AGTCCTGTTT 
GAGGACAGCG CCGCCGCTGA CCTATCGCTC TGCTCTGCCC ACCTGCTGCC GGATTCCTTG 
CCCCCCTGGG ACCTGGACCC CTCACCGCCA CCATCAGCTG GTGTACTAGA GCGGTCCAAA 
AGTAGTCGCT TCCTGTCTAC AGCAGGGCCT CTGTTTCTGG ACGAGCCTCA TTTGGTTAAG 
TCACACAGTC TGATGTTCAC ATCACGTGGG TACTATGGTG GCATGGGTGT GCAGGTGAAG 
GCGGCCGAAT ACACCAGCAT CACAGATTGC ATCGACACCC GTTGCGTCTC AACCCCTTAC 
CCTGTGCCAG AGCGGTCAGA CTCACCTGGA GGATCTTTCA CCTTCGATAA GCCTCAAGAC 
CTTGGCGTCT CGCACCCTGA GAGAGACGCT GAGCTCAGCC ACGCTGAGTC TGACTTAGAG 
GAGCCAGCAG AGGGTTCGGG AGACACATGT AAGGTGGGTC AGAGTAGCAG TAGTGGGGTA 
ATAGGTGCAG ACCTGGGCCT GGGCCTCGCA CTAGGCCCGT TTTGCTCACC CATCTCTAGA 
CTAGAGCGAG CAAACAGCTG CTCCTCTTCA GAGGAGTCGC ACTCCAACAT CTACTCTCGA 
AAGAGCTTCT CAATAAGCGA GAGAATGGAC AAGGGCCGGG GGAGCTCCAG GAACCCTTTC 
CAGAAGGCCA GAACCGGGGC GAGGCTGGAG GGTAAGACAG ACTCACTGTC TATGAGGAAA 
ATGGCCAAAC CGTCAGCTTT CAGAAGTTTT GACAGCAGAC ACAACTACAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332147.1
CDS	394..5667
Translation	MWGPFPDGGNVRPGAMNQLDAPRPLNWTIRKLCHAAFLPSVRLLKAQKSWIERAFSKRECVHIIVSTKDHHRCCCGRLIGQHVGLPPSISSNQNEKSERVPKNDSLSEKWSISKHTQLSPTDAFGTIEFQGGGHSNKAMYVRVSYDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLVGKDVVRPYQTMSNPLSKLTVLNSLHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGIINESLRDQLLVTIQKTFTYSRTQAQHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQSMLDALVLDRVDFVKLLIENGVSMHRFLTLSRLEELYNTRHGPSNTLYHLVRDVKKGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDMPIRRGRQKTTRKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNQNSREFGQLAVELLDQSYKQDEQMAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGLLLPPSILSLEFKNKDEMSYMPQDQDTYLQEKDVDEPEKQAKEKEEEDMEFTVRSYCETQYNSVAMLGNVSSEASRKKQVEEVQNRHRLIPLGRKIYEFYNAPIVKFWFHTLAYVGYLMLFNYIVLVKMDLWPSPQEWIVIAYIFTNGIEKMREILMSEPGKLLQKVKVWLQEYWNITDLMAILIFSIGMVLRLQDPPLMSYGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDHVNSRKLHPKVNDKLWLVENYMRTQGTGRYSGPSHIAPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHITMVLKHLCCRWRKHDEDERDYGLKLFITEDELKKVHDFEEQCMEEYFREKDDRFNSSNDERIRVTSERVENMAMRLEEVNEREHFMKASLQTVDIRLAQMEEMIGRIAVALERVAGMDRGEVNKARSRTSSDCTDTNYILRQSSFNSQEGNSYRLQESLEQGGDESISPTSPTALAPRVRSHSFYVSHSSKDRSGADRGEGFFKDKLFSLHRANSSQSVSSGAGPKESKPTPLNTLSVQQQLRPSSCIDIYVSASEDVPPTESFLDSVRTVPTLARDSSLHSEIMEAVLSGGRDCSGRAGGSERQSDGTVLFEDSAAADLSLCSAHLLPDSLPPWDLDPSPPPSAGVLERSKSSRFLSTAGPLFLDEPHLVKSHSLMFTSRGYYGGMGVQVKAAEYTSITDCIDTRCVSTPYPVPERSDSPGGSFTFDKPQDLGVSHPERDAELSHAESDLEEPAEGSGDTCKVGQSSSSGVIGADLGLGLALGPFCSPISRLERANSCSSSEESHSNIYSRKSFSISERMDKGRGSSRNPFQKARTGARLEGKTDSLSMRKMAKPSAFRSFDSRHNYT

Target ORF information:

RefSeq Version	XM_021476472.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476472.1

ORF Insert Sequence:

ATGTGGGGCC CTTTTCCGGA TGGCGGGAAC GTCCGGCCGG GAGCGATGAA CCAGCTGGAT 
GCTCCTCGGC CACTCAACTG GACCATACGG AAACTGTGCC ATGCAGCCTT CCTCCCGTCT 
GTGCGCCTGC TTAAGGCACA GAAATCATGG ATAGAAAGAG CGTTCAGCAA GAGAGAATGT 
GTGCACATTA TTGTAAGCAC CAAAGACCAC CATAGGTGCT GTTGTGGCCG TCTAATTGGT 
CAACATGTTG GCCTGCCCCC CAGCATCTCA TCCAATCAGA ATGAGAAGTC AGAACGCGTG 
CCAAAGAATG ACAGCTTGTC AGAGAAGTGG TCCATCAGCA AGCACACCCA GCTCAGTCCC 
ACCGACGCTT TTGGGACCAT CGAGTTCCAG GGAGGAGGAC ACTCTAATAA AGCAATGTAT 
GTACGAGTGT CTTACGACAC AAAACCAGAT TTACTGCTGC ATCTGATGAC AAAGGAGTGG 
CAACTGGAGC TTCCCAAGCT GCTCATCTCT GTTCACGGAG GACTTCAAAA CTTTGAGTTG 
CAGCCCAAAC TAAAGCAAGT CTTCGGCAAA GGTCTTATAA AGGCTGCAAT GACTACCGGA 
GCCTGGATCT TCACTGGAGG AGTTAATACT GGAGTCATCA GACATGTTGG TGATGCTCTG 
AAAGATCATG CTTCCAAATC AAGAGGGAAG ATCTGCACTA TCGGCATTGC CCCCTGGGGG 
ATTGTGGAGA ACCAGGAAGA CCTGGTGGGC AAAGATGTGG TCCGGCCATA CCAGACGATG 
TCTAACCCTC TTAGCAAGCT AACAGTACTC AACAGCCTAC ACTCACACTT CATTTTGGCT 
GATAATGGCA CCACCGGAAA GTACGGAGCA GAAGTCAAAC TGCGCAGGCA GCTGGAGAAG 
CACATCTCTC TGCAAAAGAT TAACACACGG ATTGGACAGG GTGTTCCAGT AGTGGCTCTG 
ATAGTGGAGG GGGGTCCGAA TGTCATCTCT ATCGTCCTGG AGTACCTAAG AGACACCCCT 
CCGGTGCCTG TTGTGGTGTG TGACGGCAGC GGTCGAGCCT CTGACATCCT GGCCTTTGGG 
CACAAGTACT CAGAGGAGGG CGGAATCATA AACGAATCTT TGAGAGACCA GTTATTAGTC 
ACCATTCAGA AGACGTTCAC ATACAGCCGG ACGCAGGCCC AGCATCTCTT CATCATTCTG 
ATGGAGTGCA TGAAGAAGAA AGAACTGATC ACAGTGTTTC GGATGGGATC AGAGGGACAC 
CAGGACATCG ATCTAGCCAT TCTAACAGCT CTTCTGAAAG GAGCGAATGC ATCAGCTCCA 
GATCAGCTAA GTCTGGCTTT GGCCTGGAAC AGAGTTGACA TCGCCCGGAG CCAGATCTTC 
ATTTACGGAC AGCAGTGGCC TGTTGGCTCT CTGGAGCAGT CTATGCTGGA CGCGCTGGTG 
CTGGACCGAG TGGACTTTGT AAAGCTGTTG ATTGAGAACG GAGTGAGCAT GCACCGTTTC 
CTGACACTCT CCAGACTTGA AGAGCTTTAT AATACGAGGC ACGGACCATC CAACACCTTG 
TACCACCTTG TCAGAGATGT GAAAAAGGGT AACCTGCCCC CCGACTACCG CATTAGCCTG 
ATTGACATCG GATTGGTTAT TGAATACCTC ATGGGTGGAG CCTATCGCTG CAATTACACC 
AGGAAGAGAT TTCGGACCCT CTATCACAAC CTCTTTGGAC CCAAAAGACC AAAAGCCCTG 
AAGCTGCTTG GAATGGAGGA TGACATGCCC ATTAGACGAG GGCGTCAGAA GACTACGCGG 
AAGCGAGAGG AGGAAGTAGA TATTGACCTG GATGACCCAG AAATCAATCA TTTCCCTTTC 
CCATTCCATG AGCTGATGGT TTGGGCTGTG CTGATGAAAC GGCAGAAGAT GGCACTGTTC 
TTCTGGCAGC ATGGAGAGGA GGCCATGGCT AAAGCGCTGG TGGCCTGCAA GCTCTGTAAG 
GCAATGGCTC ACGAAGCTTC TGAGAACGAC ATGGTCGATG ACATTTCACA GGAGCTCAAT 
CAAAACTCAA GGGAGTTTGG TCAGCTGGCT GTTGAGCTGC TCGATCAGTC CTATAAGCAG 
GATGAACAGA TGGCTATGAA GCTGTTGACC TACGAGCTGA AGAACTGGAG TAATGCCACC 
TGTCTGCAGC TGGCAGTGGC TGCCAAGCAC CGAGACTTCA TCGCCCACAC CTGTAGCCAG 
ATGCTGCTCA CAGACATGTG GATGGGACGT CTGCGCATGC GCAAGAACTC TGGCCTGAAG 
GTTATCTTGG GCCTGCTTCT CCCCCCTTCC ATATTGAGTC TGGAGTTTAA AAATAAAGAC 
GAGATGTCAT ACATGCCTCA GGATCAGGAC ACATATCTGC AGGAGAAGGA TGTGGATGAG 
CCCGAGAAAC AAGCTAAGGA GAAAGAGGAG GAGGACATGG AGTTCACAGT AAGATCTTAC 
TGTGAGACGC AGTACAACTC CGTGGCTATG TTGGGAAACG TCAGTTCAGA AGCATCGCGC 
AAGAAACAGG TGGAGGAGGT TCAGAACCGG CATCGGCTCA TTCCATTGGG TCGCAAAATA 
TATGAGTTTT ACAACGCCCC TATCGTCAAG TTCTGGTTTC ACACGCTGGC GTATGTAGGA 
TACTTGATGC TCTTCAACTA TATTGTGCTG GTGAAGATGG ACCTGTGGCC TTCTCCTCAG 
GAGTGGATTG TTATTGCCTA CATATTCACC AATGGCATCG AGAAGATGAG AGAGATCCTG 
ATGTCCGAAC CTGGGAAGCT GCTTCAGAAG GTGAAAGTGT GGTTACAGGA GTACTGGAAC 
ATCACTGACC TCATGGCCAT CCTCATCTTC TCCATCGGGA TGGTGCTTCG TCTGCAGGAC 
CCGCCGCTGA TGAGCTACGG CAGGGTCATT TACTGCGTCA ATATCATTTA TTGGTACATA 
CGACTCCTCG ACATCTTCGG AGTGAACAAA TACCTGGGTC CATACGTCAT GATGATCGGC 
AAGATGATGA TTGACATGAT GTATTTTGTG ATCATCATGT TGGTGGTGCT GATGAGTTTC 
GGTGTGGCGC GTCAGGCCAT CCTTAATCCC AATGAAGACC CATCCTGGAT GCTGGCACGC 
AATATCTTCT TCATGCCATA CTGGATGATC TACGGAGAGG TGTTTGCTGA CCAAATCGAC 
CATGTGAATA GTAGGAAGCT ACATCCCAAA GTGAATGACA AACTATGGCT GGTCGAAAAT 
TACATGCGCA CTCAAGGAAC TGGGCGTTAC AGTGGTCCAT CCCATATAGC TCCTTGTGGT 
CAGAACATCA CCACAGAAGA AGGAGTTGTC ATGCCTCTTC CACCCTGCAA AACTGGAGCT 
TGGATTGTCC CTGCCATAAT GGCCTGCTAC CTGCTGGTGG CCAACATCCT GCTAGTTAAC 
TTGCTCATTG CTGTCTTTAA TAACACTTTC TTTGAAGTCA AGTCCATTTC CAACCAGGTG 
TGGAAGTTTC AGAGGTATCA GCTGATCATG ACCTTCCATG AACGCCCGGT CCTTCCTCCT 
CCACTCATCA TCTTCAGCCA CATAACTATG GTTCTCAAAC ACCTGTGCTG TCGCTGGAGG 
AAGCACGATG AGGATGAACG GGACTATGGG CTGAAGCTTT TCATCACTGA AGATGAACTG 
AAGAAAGTGC ATGACTTTGA AGAGCAGTGC ATGGAGGAGT ATTTCAGAGA GAAAGATGAC 
CGCTTCAATT CCTCCAATGA CGAAAGGATA CGTGTGACAT CTGAAAGGGT GGAGAACATG 
GCGATGCGAT TAGAGGAGGT GAATGAGAGG GAACATTTTA TGAAAGCGTC CTTGCAGACT 
GTAGACATCC GGCTCGCCCA GATGGAGGAG ATGATCGGCC GCATTGCTGT GGCCCTAGAG 
AGAGTTGCTG GAATGGACAG AGGCGAAGTT AACAAAGCCC GGTCCAGGAC ATCATCAGAC 
TGCACTGATA CCAATTACAT CCTGCGCCAA AGCAGTTTCA ACAGCCAAGA AGGCAATTCC 
TACAGGCTAC AGGAGTCTCT GGAGCAGGGG GGCGACGAGT CCATTTCTCC TACTTCCCCA 
ACCGCCCTGG CTCCCCGTGT CCGCAGTCAC TCCTTCTACG TGAGCCACTC TTCCAAAGAC 
AGGAGTGGGG CGGACCGTGG AGAGGGCTTC TTTAAAGACA AGTTGTTCAG CCTCCACCGG 
GCCAACAGCT CACAGTCTGT GTCCTCAGGA GCAGGTCCCA AAGAGTCCAA GCCAACCCCT 
TTGAATACTT TATCCGTGCA GCAGCAGCTC CGCCCTTCTT CCTGCATAGA CATCTATGTT 
TCTGCCTCAG AGGACGTCCC GCCCACTGAG AGCTTCTTGG ATTCGGTTCG GACAGTTCCA 
ACTCTGGCTC GAGACTCATC TCTTCATTCG GAGATCATGG AGGCGGTGCT CTCAGGGGGA 
CGGGACTGTA GTGGAAGGGC AGGCGGCAGC GAAAGACAGT CGGATGGTAC AGTCCTGTTT 
GAGGACAGCG CCGCCGCTGA CCTATCGCTC TGCTCTGCCC ACCTGCTGCC GGATTCCTTG 
CCCCCCTGGG ACCTGGACCC CTCACCGCCA CCATCAGCTG GTGTACTAGA GCGGTCCAAA 
AGTAGTCGCT TCCTGTCTAC AGCAGGGCCT CTGTTTCTGG ACGAGCCTCA TTTGGTTAAG 
TCACACAGTC TGATGTTCAC ATCACGTGGG TACTATGGTG GCATGGGTGT GCAGGTGAAG 
GCGGCCGAAT ACACCAGCAT CACAGATTGC ATCGACACCC GTTGCGTCTC AACCCCTTAC 
CCTGTGCCAG AGCGGTCAGA CTCACCTGGA GGATCTTTCA CCTTCGATAA GCCTCAAGAC 
CTTGGCGTCT CGCACCCTGA GAGAGACGCT GAGCTCAGCC ACGCTGAGTC TGACTTAGAG 
GAGCCAGCAG AGGGTTCGGG AGACACATGT AAGGTGGGTC AGAGTAGCAG TAGTGGGGTA 
ATAGGTGCAG ACCTGGGCCT GGGCCTCGCA CTAGGCCCGT TTTGCTCACC CATCTCTAGA 
CTAGAGCGAG CAAACAGCTG CTCCTCTTCA GAGGAGTCGC ACTCCAACAT CTACTCTCGA 
AAGAGCTTCT CAATAAGCGA GAGAATGGAC AAGGGCCGGG GGAGCTCCAG GAACCCTTTC 
CAGAAGGCCA GAACCGGGGC GAGGCTGGAG GGTAAGACAG ACTCACTGTC TATGAGGAAA 
ATGGCCAAAC CGTCAGCTTT CAGAAGTTTT GACAGCAGAC ACAACTACAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm3 ( XM_021476473.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332148.1 Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X7, mRNA.

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CloneID	ODa142792
Clone ID Related Accession (Same CDS sequence)	XM_021476473.1
Accession Version	XM_021476473.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5274bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily M member 3 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGCC CTTTTCCGGA TGGCGGGAAC GTCCGGCCGG GAGCGATGAA CCAGCTGGAT 
GCTCCTCGGC CACTCAACTG GACCATACGG AAACTGTGCC ATGCAGCCTT CCTCCCGTCT 
GTGCGCCTGC TTAAGGCACA GAAATCATGG ATAGAAAGAG CGTTCAGCAA GAGAGAATGT 
GTGCACATTA TTGTAAGCAC CAAAGACCAC CATAGGTGCT GTTGTGGCCG TCTAATTGGT 
CAACATGTTG GCCTGCCCCC CAGCATCTCA TCCAATCAGA ATGAGAAGTC AGAACGCGTG 
CCAAAGAATG ACAGCTTGTC AGAGAAGTGG TCCATCAGCA AGCACACCCA GCTCAGTCCC 
ACCGACGCTT TTGGGACCAT CGAGTTCCAG GGAGGAGGAC ACTCTAATAA AGCAATGTAT 
GTACGAGTGT CTTACGACAC AAAACCAGAT TTACTGCTGC ATCTGATGAC AAAGGAGTGG 
CAACTGGAGC TTCCCAAGCT GCTCATCTCT GTTCACGGAG GACTTCAAAA CTTTGAGTTG 
CAGCCCAAAC TAAAGCAAGT CTTCGGCAAA GGTCTTATAA AGGCTGCAAT GACTACCGGA 
GCCTGGATCT TCACTGGAGG AGTTAATACT GGAGTCATCA GACATGTTGG TGATGCTCTG 
AAAGATCATG CTTCCAAATC AAGAGGGAAG ATCTGCACTA TCGGCATTGC CCCCTGGGGG 
ATTGTGGAGA ACCAGGAAGA CCTGGTGGGC AAAGATGTGG TCCGGCCATA CCAGACGATG 
TCTAACCCTC TTAGCAAGCT AACAGTACTC AACAGCCTAC ACTCACACTT CATTTTGGCT 
GATAATGGCA CCACCGGAAA GTACGGAGCA GAAGTCAAAC TGCGCAGGCA GCTGGAGAAG 
CACATCTCTC TGCAAAAGAT TAACACACGG ATTGGACAGG GTGTTCCAGT AGTGGCTCTG 
ATAGTGGAGG GGGGTCCGAA TGTCATCTCT ATCGTCCTGG AGTACCTAAG AGACACCCCT 
CCGGTGCCTG TTGTGGTGTG TGACGGCAGC GGTCGAGCCT CTGACATCCT GGCCTTTGGG 
CACAAGTACT CAGAGGAGGG CGGAATCATA AACGAATCTT TGAGAGACCA GTTATTAGTC 
ACCATTCAGA AGACGTTCAC ATACAGCCGG ACGCAGGCCC AGCATCTCTT CATCATTCTG 
ATGGAGTGCA TGAAGAAGAA AGAACTGATC ACAGTGTTTC GGATGGGATC AGAGGGACAC 
CAGGACATCG ATCTAGCCAT TCTAACAGCT CTTCTGAAAG GAGCGAATGC ATCAGCTCCA 
GATCAGCTAA GTCTGGCTTT GGCCTGGAAC AGAGTTGACA TCGCCCGGAG CCAGATCTTC 
ATTTACGGAC AGCAGTGGCC TGTTGGCTCT CTGGAGCAGT CTATGCTGGA CGCGCTGGTG 
CTGGACCGAG TGGACTTTGT AAAGCTGTTG ATTGAGAACG GAGTGAGCAT GCACCGTTTC 
CTGACACTCT CCAGACTTGA AGAGCTTTAT AATACGAGGC ACGGACCATC CAACACCTTG 
TACCACCTTG TCAGAGATGT GAAAAAGCAA GAATATCCAG GGTTCAGTTG GATCTACCTC 
AAGGGTAACC TGCCCCCCGA CTACCGCATT AGCCTGATTG ACATCGGATT GGTTATTGAA 
TACCTCATGG GTGGAGCCTA TCGCTGCAAT TACACCAGGA AGAGATTTCG GACCCTCTAT 
CACAACCTCT TTGGACCCAA AAGACCAAAA GCCCTGAAGC TGCTTGGAAT GGAGGATGAC 
ATGCCCATTA GACGAGGGCG TCAGAAGACT ACGCGGAAGC GAGAGGAGGA AGTAGATATT 
GACCTGGATG ACCCAGAAAT CAATCATTTC CCTTTCCCAT TCCATGAGCT GATGGTTTGG 
GCTGTGCTGA TGAAACGGCA GAAGATGGCA CTGTTCTTCT GGCAGCATGG AGAGGAGGCC 
ATGGCTAAAG CGCTGGTGGC CTGCAAGCTC TGTAAGGCAA TGGCTCACGA AGCTTCTGAG 
AACGACATGG TCGATGACAT TTCACAGGAG CTCAATCAAA ACTCAAGGGA GTTTGGTCAG 
CTGGCTGTTG AGCTGCTCGA TCAGTCCTAT AAGCAGGATG AACAGATGGC TATGAAGCTG 
TTGACCTACG AGCTGAAGAA CTGGAGTAAT GCCACCTGTC TGCAGCTGGC AGTGGCTGCC 
AAGCACCGAG ACTTCATCGC CCACACCTGT AGCCAGATGC TGCTCACAGA CATGTGGATG 
GGACGTCTGC GCATGCGCAA GAACTCTGGC CTGAAGGTTA TCTTGGGCCT GCTTCTCCCC 
CCTTCCATAT TGAGTCTGGA GTTTAAAAAT AAAGACGAGA TGTCATACAT GCCTCAGGAT 
CAGGACACAT ATCTGCAGGA GAAGGATGTG GATGAGCCCG AGAAACAAGC TAAGGAGAAA 
GAGGAGGAGG ACATGGAGTT CACAGCTATG TTGGGAAACG TCAGTTCAGA AGCATCGCGC 
AAGAAACAGG TGGAGGAGGT TCAGAACCGG CATCGGCTCA TTCCATTGGG TCGCAAAATA 
TATGAGTTTT ACAACGCCCC TATCGTCAAG TTCTGGTTTC ACACGCTGGC GTATGTAGGA 
TACTTGATGC TCTTCAACTA TATTGTGCTG GTGAAGATGG ACCTGTGGCC TTCTCCTCAG 
GAGTGGATTG TTATTGCCTA CATATTCACC AATGGCATCG AGAAGATGAG AGAGATCCTG 
ATGTCCGAAC CTGGGAAGCT GCTTCAGAAG GTGAAAGTGT GGTTACAGGA GTACTGGAAC 
ATCACTGACC TCATGGCCAT CCTCATCTTC TCCATCGGGA TGGTGCTTCG TCTGCAGGAC 
CCGCCGCTGA TGAGCTACGG CAGGGTCATT TACTGCGTCA ATATCATTTA TTGGTACATA 
CGACTCCTCG ACATCTTCGG AGTGAACAAA TACCTGGGTC CATACGTCAT GATGATCGGC 
AAGATGATGA TTGACATGAT GTATTTTGTG ATCATCATGT TGGTGGTGCT GATGAGTTTC 
GGTGTGGCGC GTCAGGCCAT CCTTAATCCC AATGAAGACC CATCCTGGAT GCTGGCACGC 
AATATCTTCT TCATGCCATA CTGGATGATC TACGGAGAGG TGTTTGCTGA CCAAATCGAC 
CATGTGAATA GTAGGAAGCT ACATCCCAAA GTGAATGACA AACTATGGCT GGTCGAAAAT 
TACATGCGCA CTCAAGGAAC TGGGCGTTAC AGTGGTCCAT CCCATATAGC TCCTTGTGGT 
CAGAACATCA CCACAGAAGA AGGAGTTGTC ATGCCTCTTC CACCCTGCAA AACTGGAGCT 
TGGATTGTCC CTGCCATAAT GGCCTGCTAC CTGCTGGTGG CCAACATCCT GCTAGTTAAC 
TTGCTCATTG CTGTCTTTAA TAACACTTTC TTTGAAGTCA AGTCCATTTC CAACCAGGTG 
TGGAAGTTTC AGAGGTATCA GCTGATCATG ACCTTCCATG AACGCCCGGT CCTTCCTCCT 
CCACTCATCA TCTTCAGCCA CATAACTATG GTTCTCAAAC ACCTGTGCTG TCGCTGGAGG 
AAGCACGATG AGGATGAACG GGACTATGGG CTGAAGCTTT TCATCACTGA AGATGAACTG 
AAGAAAGTGC ATGACTTTGA AGAGCAGTGC ATGGAGGAGT ATTTCAGAGA GAAAGATGAC 
CGCTTCAATT CCTCCAATGA CGAAAGGATA CGTGTGACAT CTGAAAGGGT GGAGAACATG 
GCGATGCGAT TAGAGGAGGT GAATGAGAGG GAACATTTTA TGAAAGCGTC CTTGCAGACT 
GTAGACATCC GGCTCGCCCA GATGGAGGAG ATGATCGGCC GCATTGCTGT GGCCCTAGAG 
AGAGTTGCTG GAATGGACAG AGGCGAAGTT AACAAAGCCC GGTCCAGGAC ATCATCAGAC 
TGCACTGATA CCAATTACAT CCTGCGCCAA AGCAGTTTCA ACAGCCAAGA AGGCAATTCC 
TACAGGCTAC AGGAGTCTCT GGAGCAGGGG GGCGACGAGT CCATTTCTCC TACTTCCCCA 
ACCGCCCTGG CTCCCCGTGT CCGCAGTCAC TCCTTCTACG TGAGCCACTC TTCCAAAGAC 
AGGAGTGGGG CGGACCGTGG AGAGGGCTTC TTTAAAGACA AGTTGTTCAG CCTCCACCGG 
GCCAACAGCT CACAGTCTGT GTCCTCAGGA GCAGGTCCCA AAGAGTCCAA GCCAACCCCT 
TTGAATACTT TATCCGTGCA GCAGCAGCTC CGCCCTTCTT CCTGCATAGA CATCTATGTT 
TCTGCCTCAG AGGACGTCCC GCCCACTGAG AGCTTCTTGG ATTCGGTTCG GACAGTTCCA 
ACTCTGGCTC GAGACTCATC TCTTCATTCG GAGATCATGG AGGCGGTGCT CTCAGGGGGA 
CGGGACTGTA GTGGAAGGGC AGGCGGCAGC GAAAGACAGT CGGATGGTAC AGTCCTGTTT 
GAGGACAGCG CCGCCGCTGA CCTATCGCTC TGCTCTGCCC ACCTGCTGCC GGATTCCTTG 
CCCCCCTGGG ACCTGGACCC CTCACCGCCA CCATCAGCTG GTGTACTAGA GCGGTCCAAA 
AGTAGTCGCT TCCTGTCTAC AGCAGGGCCT CTGTTTCTGG ACGAGCCTCA TTTGGTTAAG 
TCACACAGTC TGATGTTCAC ATCACGTGGG TACTATGGTG GCATGGGTGT GCAGGTGAAG 
GCGGCCGAAT ACACCAGCAT CACAGATTGC ATCGACACCC GTTGCGTCTC AACCCCTTAC 
CCTGTGCCAG AGCGGTCAGA CTCACCTGGA GGATCTTTCA CCTTCGATAA GCCTCAAGAC 
CTTGGCGTCT CGCACCCTGA GAGAGACGCT GAGCTCAGCC ACGCTGAGTC TGACTTAGAG 
GAGCCAGCAG AGGGTTCGGG AGACACATGT AAGGTGGGTC AGAGTAGCAG TAGTGGGGTA 
ATAGGTGCAG ACCTGGGCCT GGGCCTCGCA CTAGGCCCGT TTTGCTCACC CATCTCTAGA 
CTAGAGCGAG CAAACAGCTG CTCCTCTTCA GAGGAGTCGC ACTCCAACAT CTACTCTCGA 
AAGAGCTTCT CAATAAGCGA GAGAATGGAC AAGGGCCGGG GGAGCTCCAG GAACCCTTTC 
CAGAAGGCCA GAACCGGGGC GAGGCTGGAG GGTAAGACAG ACTCACTGTC TATGAGGAAA 
ATGGCCAAAC CGTCAGCTTT CAGAAGTTTT GACAGCAGAC ACAACTACAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332148.1
CDS	399..5672
Translation	MWGPFPDGGNVRPGAMNQLDAPRPLNWTIRKLCHAAFLPSVRLLKAQKSWIERAFSKRECVHIIVSTKDHHRCCCGRLIGQHVGLPPSISSNQNEKSERVPKNDSLSEKWSISKHTQLSPTDAFGTIEFQGGGHSNKAMYVRVSYDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLVGKDVVRPYQTMSNPLSKLTVLNSLHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGIINESLRDQLLVTIQKTFTYSRTQAQHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQSMLDALVLDRVDFVKLLIENGVSMHRFLTLSRLEELYNTRHGPSNTLYHLVRDVKKQEYPGFSWIYLKGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDMPIRRGRQKTTRKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNQNSREFGQLAVELLDQSYKQDEQMAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGLLLPPSILSLEFKNKDEMSYMPQDQDTYLQEKDVDEPEKQAKEKEEEDMEFTAMLGNVSSEASRKKQVEEVQNRHRLIPLGRKIYEFYNAPIVKFWFHTLAYVGYLMLFNYIVLVKMDLWPSPQEWIVIAYIFTNGIEKMREILMSEPGKLLQKVKVWLQEYWNITDLMAILIFSIGMVLRLQDPPLMSYGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDHVNSRKLHPKVNDKLWLVENYMRTQGTGRYSGPSHIAPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHITMVLKHLCCRWRKHDEDERDYGLKLFITEDELKKVHDFEEQCMEEYFREKDDRFNSSNDERIRVTSERVENMAMRLEEVNEREHFMKASLQTVDIRLAQMEEMIGRIAVALERVAGMDRGEVNKARSRTSSDCTDTNYILRQSSFNSQEGNSYRLQESLEQGGDESISPTSPTALAPRVRSHSFYVSHSSKDRSGADRGEGFFKDKLFSLHRANSSQSVSSGAGPKESKPTPLNTLSVQQQLRPSSCIDIYVSASEDVPPTESFLDSVRTVPTLARDSSLHSEIMEAVLSGGRDCSGRAGGSERQSDGTVLFEDSAAADLSLCSAHLLPDSLPPWDLDPSPPPSAGVLERSKSSRFLSTAGPLFLDEPHLVKSHSLMFTSRGYYGGMGVQVKAAEYTSITDCIDTRCVSTPYPVPERSDSPGGSFTFDKPQDLGVSHPERDAELSHAESDLEEPAEGSGDTCKVGQSSSSGVIGADLGLGLALGPFCSPISRLERANSCSSSEESHSNIYSRKSFSISERMDKGRGSSRNPFQKARTGARLEGKTDSLSMRKMAKPSAFRSFDSRHNYT

Target ORF information:

RefSeq Version	XM_021476473.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476473.1

ORF Insert Sequence:

ATGTGGGGCC CTTTTCCGGA TGGCGGGAAC GTCCGGCCGG GAGCGATGAA CCAGCTGGAT 
GCTCCTCGGC CACTCAACTG GACCATACGG AAACTGTGCC ATGCAGCCTT CCTCCCGTCT 
GTGCGCCTGC TTAAGGCACA GAAATCATGG ATAGAAAGAG CGTTCAGCAA GAGAGAATGT 
GTGCACATTA TTGTAAGCAC CAAAGACCAC CATAGGTGCT GTTGTGGCCG TCTAATTGGT 
CAACATGTTG GCCTGCCCCC CAGCATCTCA TCCAATCAGA ATGAGAAGTC AGAACGCGTG 
CCAAAGAATG ACAGCTTGTC AGAGAAGTGG TCCATCAGCA AGCACACCCA GCTCAGTCCC 
ACCGACGCTT TTGGGACCAT CGAGTTCCAG GGAGGAGGAC ACTCTAATAA AGCAATGTAT 
GTACGAGTGT CTTACGACAC AAAACCAGAT TTACTGCTGC ATCTGATGAC AAAGGAGTGG 
CAACTGGAGC TTCCCAAGCT GCTCATCTCT GTTCACGGAG GACTTCAAAA CTTTGAGTTG 
CAGCCCAAAC TAAAGCAAGT CTTCGGCAAA GGTCTTATAA AGGCTGCAAT GACTACCGGA 
GCCTGGATCT TCACTGGAGG AGTTAATACT GGAGTCATCA GACATGTTGG TGATGCTCTG 
AAAGATCATG CTTCCAAATC AAGAGGGAAG ATCTGCACTA TCGGCATTGC CCCCTGGGGG 
ATTGTGGAGA ACCAGGAAGA CCTGGTGGGC AAAGATGTGG TCCGGCCATA CCAGACGATG 
TCTAACCCTC TTAGCAAGCT AACAGTACTC AACAGCCTAC ACTCACACTT CATTTTGGCT 
GATAATGGCA CCACCGGAAA GTACGGAGCA GAAGTCAAAC TGCGCAGGCA GCTGGAGAAG 
CACATCTCTC TGCAAAAGAT TAACACACGG ATTGGACAGG GTGTTCCAGT AGTGGCTCTG 
ATAGTGGAGG GGGGTCCGAA TGTCATCTCT ATCGTCCTGG AGTACCTAAG AGACACCCCT 
CCGGTGCCTG TTGTGGTGTG TGACGGCAGC GGTCGAGCCT CTGACATCCT GGCCTTTGGG 
CACAAGTACT CAGAGGAGGG CGGAATCATA AACGAATCTT TGAGAGACCA GTTATTAGTC 
ACCATTCAGA AGACGTTCAC ATACAGCCGG ACGCAGGCCC AGCATCTCTT CATCATTCTG 
ATGGAGTGCA TGAAGAAGAA AGAACTGATC ACAGTGTTTC GGATGGGATC AGAGGGACAC 
CAGGACATCG ATCTAGCCAT TCTAACAGCT CTTCTGAAAG GAGCGAATGC ATCAGCTCCA 
GATCAGCTAA GTCTGGCTTT GGCCTGGAAC AGAGTTGACA TCGCCCGGAG CCAGATCTTC 
ATTTACGGAC AGCAGTGGCC TGTTGGCTCT CTGGAGCAGT CTATGCTGGA CGCGCTGGTG 
CTGGACCGAG TGGACTTTGT AAAGCTGTTG ATTGAGAACG GAGTGAGCAT GCACCGTTTC 
CTGACACTCT CCAGACTTGA AGAGCTTTAT AATACGAGGC ACGGACCATC CAACACCTTG 
TACCACCTTG TCAGAGATGT GAAAAAGCAA GAATATCCAG GGTTCAGTTG GATCTACCTC 
AAGGGTAACC TGCCCCCCGA CTACCGCATT AGCCTGATTG ACATCGGATT GGTTATTGAA 
TACCTCATGG GTGGAGCCTA TCGCTGCAAT TACACCAGGA AGAGATTTCG GACCCTCTAT 
CACAACCTCT TTGGACCCAA AAGACCAAAA GCCCTGAAGC TGCTTGGAAT GGAGGATGAC 
ATGCCCATTA GACGAGGGCG TCAGAAGACT ACGCGGAAGC GAGAGGAGGA AGTAGATATT 
GACCTGGATG ACCCAGAAAT CAATCATTTC CCTTTCCCAT TCCATGAGCT GATGGTTTGG 
GCTGTGCTGA TGAAACGGCA GAAGATGGCA CTGTTCTTCT GGCAGCATGG AGAGGAGGCC 
ATGGCTAAAG CGCTGGTGGC CTGCAAGCTC TGTAAGGCAA TGGCTCACGA AGCTTCTGAG 
AACGACATGG TCGATGACAT TTCACAGGAG CTCAATCAAA ACTCAAGGGA GTTTGGTCAG 
CTGGCTGTTG AGCTGCTCGA TCAGTCCTAT AAGCAGGATG AACAGATGGC TATGAAGCTG 
TTGACCTACG AGCTGAAGAA CTGGAGTAAT GCCACCTGTC TGCAGCTGGC AGTGGCTGCC 
AAGCACCGAG ACTTCATCGC CCACACCTGT AGCCAGATGC TGCTCACAGA CATGTGGATG 
GGACGTCTGC GCATGCGCAA GAACTCTGGC CTGAAGGTTA TCTTGGGCCT GCTTCTCCCC 
CCTTCCATAT TGAGTCTGGA GTTTAAAAAT AAAGACGAGA TGTCATACAT GCCTCAGGAT 
CAGGACACAT ATCTGCAGGA GAAGGATGTG GATGAGCCCG AGAAACAAGC TAAGGAGAAA 
GAGGAGGAGG ACATGGAGTT CACAGCTATG TTGGGAAACG TCAGTTCAGA AGCATCGCGC 
AAGAAACAGG TGGAGGAGGT TCAGAACCGG CATCGGCTCA TTCCATTGGG TCGCAAAATA 
TATGAGTTTT ACAACGCCCC TATCGTCAAG TTCTGGTTTC ACACGCTGGC GTATGTAGGA 
TACTTGATGC TCTTCAACTA TATTGTGCTG GTGAAGATGG ACCTGTGGCC TTCTCCTCAG 
GAGTGGATTG TTATTGCCTA CATATTCACC AATGGCATCG AGAAGATGAG AGAGATCCTG 
ATGTCCGAAC CTGGGAAGCT GCTTCAGAAG GTGAAAGTGT GGTTACAGGA GTACTGGAAC 
ATCACTGACC TCATGGCCAT CCTCATCTTC TCCATCGGGA TGGTGCTTCG TCTGCAGGAC 
CCGCCGCTGA TGAGCTACGG CAGGGTCATT TACTGCGTCA ATATCATTTA TTGGTACATA 
CGACTCCTCG ACATCTTCGG AGTGAACAAA TACCTGGGTC CATACGTCAT GATGATCGGC 
AAGATGATGA TTGACATGAT GTATTTTGTG ATCATCATGT TGGTGGTGCT GATGAGTTTC 
GGTGTGGCGC GTCAGGCCAT CCTTAATCCC AATGAAGACC CATCCTGGAT GCTGGCACGC 
AATATCTTCT TCATGCCATA CTGGATGATC TACGGAGAGG TGTTTGCTGA CCAAATCGAC 
CATGTGAATA GTAGGAAGCT ACATCCCAAA GTGAATGACA AACTATGGCT GGTCGAAAAT 
TACATGCGCA CTCAAGGAAC TGGGCGTTAC AGTGGTCCAT CCCATATAGC TCCTTGTGGT 
CAGAACATCA CCACAGAAGA AGGAGTTGTC ATGCCTCTTC CACCCTGCAA AACTGGAGCT 
TGGATTGTCC CTGCCATAAT GGCCTGCTAC CTGCTGGTGG CCAACATCCT GCTAGTTAAC 
TTGCTCATTG CTGTCTTTAA TAACACTTTC TTTGAAGTCA AGTCCATTTC CAACCAGGTG 
TGGAAGTTTC AGAGGTATCA GCTGATCATG ACCTTCCATG AACGCCCGGT CCTTCCTCCT 
CCACTCATCA TCTTCAGCCA CATAACTATG GTTCTCAAAC ACCTGTGCTG TCGCTGGAGG 
AAGCACGATG AGGATGAACG GGACTATGGG CTGAAGCTTT TCATCACTGA AGATGAACTG 
AAGAAAGTGC ATGACTTTGA AGAGCAGTGC ATGGAGGAGT ATTTCAGAGA GAAAGATGAC 
CGCTTCAATT CCTCCAATGA CGAAAGGATA CGTGTGACAT CTGAAAGGGT GGAGAACATG 
GCGATGCGAT TAGAGGAGGT GAATGAGAGG GAACATTTTA TGAAAGCGTC CTTGCAGACT 
GTAGACATCC GGCTCGCCCA GATGGAGGAG ATGATCGGCC GCATTGCTGT GGCCCTAGAG 
AGAGTTGCTG GAATGGACAG AGGCGAAGTT AACAAAGCCC GGTCCAGGAC ATCATCAGAC 
TGCACTGATA CCAATTACAT CCTGCGCCAA AGCAGTTTCA ACAGCCAAGA AGGCAATTCC 
TACAGGCTAC AGGAGTCTCT GGAGCAGGGG GGCGACGAGT CCATTTCTCC TACTTCCCCA 
ACCGCCCTGG CTCCCCGTGT CCGCAGTCAC TCCTTCTACG TGAGCCACTC TTCCAAAGAC 
AGGAGTGGGG CGGACCGTGG AGAGGGCTTC TTTAAAGACA AGTTGTTCAG CCTCCACCGG 
GCCAACAGCT CACAGTCTGT GTCCTCAGGA GCAGGTCCCA AAGAGTCCAA GCCAACCCCT 
TTGAATACTT TATCCGTGCA GCAGCAGCTC CGCCCTTCTT CCTGCATAGA CATCTATGTT 
TCTGCCTCAG AGGACGTCCC GCCCACTGAG AGCTTCTTGG ATTCGGTTCG GACAGTTCCA 
ACTCTGGCTC GAGACTCATC TCTTCATTCG GAGATCATGG AGGCGGTGCT CTCAGGGGGA 
CGGGACTGTA GTGGAAGGGC AGGCGGCAGC GAAAGACAGT CGGATGGTAC AGTCCTGTTT 
GAGGACAGCG CCGCCGCTGA CCTATCGCTC TGCTCTGCCC ACCTGCTGCC GGATTCCTTG 
CCCCCCTGGG ACCTGGACCC CTCACCGCCA CCATCAGCTG GTGTACTAGA GCGGTCCAAA 
AGTAGTCGCT TCCTGTCTAC AGCAGGGCCT CTGTTTCTGG ACGAGCCTCA TTTGGTTAAG 
TCACACAGTC TGATGTTCAC ATCACGTGGG TACTATGGTG GCATGGGTGT GCAGGTGAAG 
GCGGCCGAAT ACACCAGCAT CACAGATTGC ATCGACACCC GTTGCGTCTC AACCCCTTAC 
CCTGTGCCAG AGCGGTCAGA CTCACCTGGA GGATCTTTCA CCTTCGATAA GCCTCAAGAC 
CTTGGCGTCT CGCACCCTGA GAGAGACGCT GAGCTCAGCC ACGCTGAGTC TGACTTAGAG 
GAGCCAGCAG AGGGTTCGGG AGACACATGT AAGGTGGGTC AGAGTAGCAG TAGTGGGGTA 
ATAGGTGCAG ACCTGGGCCT GGGCCTCGCA CTAGGCCCGT TTTGCTCACC CATCTCTAGA 
CTAGAGCGAG CAAACAGCTG CTCCTCTTCA GAGGAGTCGC ACTCCAACAT CTACTCTCGA 
AAGAGCTTCT CAATAAGCGA GAGAATGGAC AAGGGCCGGG GGAGCTCCAG GAACCCTTTC 
CAGAAGGCCA GAACCGGGGC GAGGCTGGAG GGTAAGACAG ACTCACTGTC TATGAGGAAA 
ATGGCCAAAC CGTCAGCTTT CAGAAGTTTT GACAGCAGAC ACAACTACAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm3 ( XM_021476474.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332149.1 Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X9, mRNA.

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CloneID	ODa142793
Clone ID Related Accession (Same CDS sequence)	XM_021476474.1
Accession Version	XM_021476474.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily M member 3 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGCC CTTTTCCGGA TGGCGGGAAC GTCCGGCCGG GAGCGATGAA CCAGCTGGAT 
GCTCCTCGGC CACTCAACTG GACCATACGG AAACTGTGCC ATGCAGCCTT CCTCCCGTCT 
GTGCGCCTGC TTAAGGCACA GAAATCATGG ATAGAAAGAG CGTTCAGCAA GAGAGAATGT 
GTGCACATTA TTGTAAGCAC CAAAGACCAC CATAGGTGCT GTTGTGGCCG TCTAATTGGT 
CAACATGTTG GCCTGCCCCC CAGCATCTCA TCCAATCAGA ATGAGAAGTC AGAACGCGTG 
CCAAAGAATG ACAGCTTGTC AGAGAAGTGG TCCATCAGCA AGCACACCCA GCTCAGTCCC 
ACCGACGCTT TTGGGACCAT CGAGTTCCAG GGAGGAGGAC ACTCTAATAA AGCAATGTAT 
GTACGAGTGT CTTACGACAC AAAACCAGAT TTACTGCTGC ATCTGATGAC AAAGGAGTGG 
CAACTGGAGC TTCCCAAGCT GCTCATCTCT GTTCACGGAG GACTTCAAAA CTTTGAGTTG 
CAGCCCAAAC TAAAGCAAGT CTTCGGCAAA GGTCTTATAA AGGCTGCAAT GACTACCGGA 
GCCTGGATCT TCACTGGAGG AGTTAATACT GGAGTCATCA GACATGTTGG TGATGCTCTG 
AAAGATCATG CTTCCAAATC AAGAGGGAAG ATCTGCACTA TCGGCATTGC CCCCTGGGGG 
ATTGTGGAGA ACCAGGAAGA CCTGGTGGGC AAAGATGTGG TCCGGCCATA CCAGACGATG 
TCTAACCCTC TTAGCAAGCT AACAGTACTC AACAGCCTAC ACTCACACTT CATTTTGGCT 
GATAATGGCA CCACCGGAAA GTACGGAGCA GAAGTCAAAC TGCGCAGGCA GCTGGAGAAG 
CACATCTCTC TGCAAAAGAT TAACACACGG ATTGGACAGG GTGTTCCAGT AGTGGCTCTG 
ATAGTGGAGG GGGGTCCGAA TGTCATCTCT ATCGTCCTGG AGTACCTAAG AGACACCCCT 
CCGGTGCCTG TTGTGGTGTG TGACGGCAGC GGTCGAGCCT CTGACATCCT GGCCTTTGGG 
CACAAGTACT CAGAGGAGGG CGGAATCATA AACGAATCTT TGAGAGACCA GTTATTAGTC 
ACCATTCAGA AGACGTTCAC ATACAGCCGG ACGCAGGCCC AGCATCTCTT CATCATTCTG 
ATGGAGTGCA TGAAGAAGAA AGAACTGATC ACAGTGTTTC GGATGGGATC AGAGGGACAC 
CAGGACATCG ATCTAGCCAT TCTAACAGCT CTTCTGAAAG GAGCGAATGC ATCAGCTCCA 
GATCAGCTAA GTCTGGCTTT GGCCTGGAAC AGAGTTGACA TCGCCCGGAG CCAGATCTTC 
ATTTACGGAC AGCAGTGGCC TGTTGGCTCT CTGGAGCAGT CTATGCTGGA CGCGCTGGTG 
CTGGACCGAG TGGACTTTGT AAAGCTGTTG ATTGAGAACG GAGTGAGCAT GCACCGTTTC 
CTGACACTCT CCAGACTTGA AGAGCTTTAT AATACGAGGC ACGGACCATC CAACACCTTG 
TACCACCTTG TCAGAGATGT GAAAAAGGGT AACCTGCCCC CCGACTACCG CATTAGCCTG 
ATTGACATCG GATTGGTTAT TGAATACCTC ATGGGTGGAG CCTATCGCTG CAATTACACC 
AGGAAGAGAT TTCGGACCCT CTATCACAAC CTCTTTGGAC CCAAAAGACC AAAAGCCCTG 
AAGCTGCTTG GAATGGAGGA TGACATGCCC ATTAGACGAG GGCGTCAGAA GACTACGCGG 
AAGCGAGAGG AGGAAGTAGA TATTGACCTG GATGACCCAG AAATCAATCA TTTCCCTTTC 
CCATTCCATG AGCTGATGGT TTGGGCTGTG CTGATGAAAC GGCAGAAGAT GGCACTGTTC 
TTCTGGCAGC ATGGAGAGGA GGCCATGGCT AAAGCGCTGG TGGCCTGCAA GCTCTGTAAG 
GCAATGGCTC ACGAAGCTTC TGAGAACGAC ATGGTCGATG ACATTTCACA GGAGCTCAAT 
CAAAACTCAA GGGAGTTTGG TCAGCTGGCT GTTGAGCTGC TCGATCAGTC CTATAAGCAG 
GATGAACAGA TGGCTATGAA GCTGTTGACC TACGAGCTGA AGAACTGGAG TAATGCCACC 
TGTCTGCAGC TGGCAGTGGC TGCCAAGCAC CGAGACTTCA TCGCCCACAC CTGTAGCCAG 
ATGCTGCTCA CAGACATGTG GATGGGACGT CTGCGCATGC GCAAGAACTC TGGCCTGAAG 
GTTATCTTGG GCCTGCTTCT CCCCCCTTCC ATATTGAGTC TGGAGTTTAA AAATAAAGAC 
GAGATGTCAT ACATGCCTCA GGATCAGGAC ACATATCTGC AGGAGAAGGA TGTGGATGAG 
CCCGAGAAAC AAGCTAAGGA GAAAGAGGAG GAGGACATGG AGTTCACAGC TATGTTGGGA 
AACGTCAGTT CAGAAGCATC GCGCAAGAAA CAGGTGGAGG AGGTTCAGAA CCGGCATCGG 
CTCATTCCAT TGGGTCGCAA AATATATGAG TTTTACAACG CCCCTATCGT CAAGTTCTGG 
TTTCACACGC TGGCGTATGT AGGATACTTG ATGCTCTTCA ACTATATTGT GCTGGTGAAG 
ATGGACCTGT GGCCTTCTCC TCAGGAGTGG ATTGTTATTG CCTACATATT CACCAATGGC 
ATCGAGAAGA TGAGAGAGAT CCTGATGTCC GAACCTGGGA AGCTGCTTCA GAAGGTGAAA 
GTGTGGTTAC AGGAGTACTG GAACATCACT GACCTCATGG CCATCCTCAT CTTCTCCATC 
GGGATGGTGC TTCGTCTGCA GGACCCGCCG CTGATGAGCT ACGGCAGGGT CATTTACTGC 
GTCAATATCA TTTATTGGTA CATACGACTC CTCGACATCT TCGGAGTGAA CAAATACCTG 
GGTCCATACG TCATGATGAT CGGCAAGATG ATGATTGACA TGATGTATTT TGTGATCATC 
ATGTTGGTGG TGCTGATGAG TTTCGGTGTG GCGCGTCAGG CCATCCTTAA TCCCAATGAA 
GACCCATCCT GGATGCTGGC ACGCAATATC TTCTTCATGC CATACTGGAT GATCTACGGA 
GAGGTGTTTG CTGACCAAAT CGACCATGTG AATAGTAGGA AGCTACATCC CAAAGTGAAT 
GACAAACTAT GGCTGGTCGA AAATTACATG CGCACTCAAG GAACTGGGCG TTACAGTGGT 
CCATCCCATA TAGCTCCTTG TGGTCAGAAC ATCACCACAG AAGAAGGAGT TGTCATGCCT 
CTTCCACCCT GCAAAACTGG AGCTTGGATT GTCCCTGCCA TAATGGCCTG CTACCTGCTG 
GTGGCCAACA TCCTGCTAGT TAACTTGCTC ATTGCTGTCT TTAATAACAC TTTCTTTGAA 
GTCAAGTCCA TTTCCAACCA GGTGTGGAAG TTTCAGAGGT ATCAGCTGAT CATGACCTTC 
CATGAACGCC CGGTCCTTCC TCCTCCACTC ATCATCTTCA GCCACATAAC TATGGTTCTC 
AAACACCTGT GCTGTCGCTG GAGGAAGCAC GATGAGGATG AACGGGACTA TGGGCTGAAG 
CTTTTCATCA CTGAAGATGA ACTGAAGAAA GTGCATGACT TTGAAGAGCA GTGCATGGAG 
GAGTATTTCA GAGAGAAAGA TGACCGCTTC AATTCCTCCA ATGACGAAAG GATACGTGTG 
ACATCTGAAA GGGTGGAGAA CATGGCGATG CGATTAGAGG AGGTGAATGA GAGGGAACAT 
TTTATGAAAG CGTCCTTGCA GACTGTAGAC ATCCGGCTCG CCCAGATGGA GGAGATGATC 
GGCCGCATTG CTGTGGCCCT AGAGAGAGTT GCTGGAATGG ACAGAGGCGA AGTTAACAAA 
GCCCGGTCCA GGACATCATC AGACTGCACT GATACCAATT ACATCCTGCG CCAAAGCAGT 
TTCAACAGCC AAGAAGGCAA TTCCTACAGG CTACAGGAGT CTCTGGAGCA GGGGGGCGAC 
GAGTCCATTT CTCCTACTTC CCCAACCGCC CTGGCTCCCC GTGTCCGCAG TCACTCCTTC 
TACGTGAGCC ACTCTTCCAA AGACAGGAGT GGGGCGGACC GTGGAGAGGG CTTCTTTAAA 
GACAAGTTGT TCAGCCTCCA CCGGGCCAAC AGCTCACAGT CTGTGTCCTC AGGAGCAGGT 
CCCAAAGAGT CCAAGCCAAC CCCTTTGAAT ACTTTATCCG TGCAGCAGCA GCTCCGCCCT 
TCTTCCTGCA TAGACATCTA TGTTTCTGCC TCAGAGGACG TCCCGCCCAC TGAGAGCTTC 
TTGGATTCGG TTCGGACAGT TCCAACTCTG GCTCGAGACT CATCTCTTCA TTCGGAGATC 
ATGGAGGCGG TGCTCTCAGG GGGACGGGAC TGTAGTGGAA GGGCAGGCGG CAGCGAAAGA 
CAGTCGGATG GTACAGTCCT GTTTGAGGAC AGCGCCGCCG CTGACCTATC GCTCTGCTCT 
GCCCACCTGC TGCCGGATTC CTTGCCCCCC TGGGACCTGG ACCCCTCACC GCCACCATCA 
GCTGGTGTAC TAGAGCGGTC CAAAAGTAGT CGCTTCCTGT CTACAGCAGG GCCTCTGTTT 
CTGGACGAGC CTCATTTGGT TAAGTCACAC AGTCTGATGT TCACATCACG TGGGTACTAT 
GGTGGCATGG GTGTGCAGGT GAAGGCGGCC GAATACACCA GCATCACAGA TTGCATCGAC 
ACCCGTTGCG TCTCAACCCC TTACCCTGTG CCAGAGCGGT CAGACTCACC TGGAGGATCT 
TTCACCTTCG ATAAGCCTCA AGACCTTGGC GTCTCGCACC CTGAGAGAGA CGCTGAGCTC 
AGCCACGCTG AGTCTGACTT AGAGGAGCCA GCAGAGGGTT CGGGAGACAC ATGTAAGGTG 
GGTCAGAGTA GCAGTAGTGG GGTAATAGGT GCAGACCTGG GCCTGGGCCT CGCACTAGGC 
CCGTTTTGCT CACCCATCTC TAGACTAGAG CGAGCAAACA GCTGCTCCTC TTCAGAGGAG 
TCGCACTCCA ACATCTACTC TCGAAAGAGC TTCTCAATAA GCGAGAGAAT GGACAAGGGC 
CGGGGGAGCT CCAGGAACCC TTTCCAGAAG GCCAGAACCG GGGCGAGGCT GGAGGGTAAG 
ACAGACTCAC TGTCTATGAG GAAAATGGCC AAACCGTCAG CTTTCAGAAG TTTTGACAGC 
AGACACAACT ACACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332149.1
CDS	395..5632
Translation	MWGPFPDGGNVRPGAMNQLDAPRPLNWTIRKLCHAAFLPSVRLLKAQKSWIERAFSKRECVHIIVSTKDHHRCCCGRLIGQHVGLPPSISSNQNEKSERVPKNDSLSEKWSISKHTQLSPTDAFGTIEFQGGGHSNKAMYVRVSYDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLVGKDVVRPYQTMSNPLSKLTVLNSLHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGIINESLRDQLLVTIQKTFTYSRTQAQHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQSMLDALVLDRVDFVKLLIENGVSMHRFLTLSRLEELYNTRHGPSNTLYHLVRDVKKGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDMPIRRGRQKTTRKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNQNSREFGQLAVELLDQSYKQDEQMAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGLLLPPSILSLEFKNKDEMSYMPQDQDTYLQEKDVDEPEKQAKEKEEEDMEFTAMLGNVSSEASRKKQVEEVQNRHRLIPLGRKIYEFYNAPIVKFWFHTLAYVGYLMLFNYIVLVKMDLWPSPQEWIVIAYIFTNGIEKMREILMSEPGKLLQKVKVWLQEYWNITDLMAILIFSIGMVLRLQDPPLMSYGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDHVNSRKLHPKVNDKLWLVENYMRTQGTGRYSGPSHIAPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHITMVLKHLCCRWRKHDEDERDYGLKLFITEDELKKVHDFEEQCMEEYFREKDDRFNSSNDERIRVTSERVENMAMRLEEVNEREHFMKASLQTVDIRLAQMEEMIGRIAVALERVAGMDRGEVNKARSRTSSDCTDTNYILRQSSFNSQEGNSYRLQESLEQGGDESISPTSPTALAPRVRSHSFYVSHSSKDRSGADRGEGFFKDKLFSLHRANSSQSVSSGAGPKESKPTPLNTLSVQQQLRPSSCIDIYVSASEDVPPTESFLDSVRTVPTLARDSSLHSEIMEAVLSGGRDCSGRAGGSERQSDGTVLFEDSAAADLSLCSAHLLPDSLPPWDLDPSPPPSAGVLERSKSSRFLSTAGPLFLDEPHLVKSHSLMFTSRGYYGGMGVQVKAAEYTSITDCIDTRCVSTPYPVPERSDSPGGSFTFDKPQDLGVSHPERDAELSHAESDLEEPAEGSGDTCKVGQSSSSGVIGADLGLGLALGPFCSPISRLERANSCSSSEESHSNIYSRKSFSISERMDKGRGSSRNPFQKARTGARLEGKTDSLSMRKMAKPSAFRSFDSRHNYT

Target ORF information:

RefSeq Version	XM_021476474.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476474.1

ORF Insert Sequence:

ATGTGGGGCC CTTTTCCGGA TGGCGGGAAC GTCCGGCCGG GAGCGATGAA CCAGCTGGAT 
GCTCCTCGGC CACTCAACTG GACCATACGG AAACTGTGCC ATGCAGCCTT CCTCCCGTCT 
GTGCGCCTGC TTAAGGCACA GAAATCATGG ATAGAAAGAG CGTTCAGCAA GAGAGAATGT 
GTGCACATTA TTGTAAGCAC CAAAGACCAC CATAGGTGCT GTTGTGGCCG TCTAATTGGT 
CAACATGTTG GCCTGCCCCC CAGCATCTCA TCCAATCAGA ATGAGAAGTC AGAACGCGTG 
CCAAAGAATG ACAGCTTGTC AGAGAAGTGG TCCATCAGCA AGCACACCCA GCTCAGTCCC 
ACCGACGCTT TTGGGACCAT CGAGTTCCAG GGAGGAGGAC ACTCTAATAA AGCAATGTAT 
GTACGAGTGT CTTACGACAC AAAACCAGAT TTACTGCTGC ATCTGATGAC AAAGGAGTGG 
CAACTGGAGC TTCCCAAGCT GCTCATCTCT GTTCACGGAG GACTTCAAAA CTTTGAGTTG 
CAGCCCAAAC TAAAGCAAGT CTTCGGCAAA GGTCTTATAA AGGCTGCAAT GACTACCGGA 
GCCTGGATCT TCACTGGAGG AGTTAATACT GGAGTCATCA GACATGTTGG TGATGCTCTG 
AAAGATCATG CTTCCAAATC AAGAGGGAAG ATCTGCACTA TCGGCATTGC CCCCTGGGGG 
ATTGTGGAGA ACCAGGAAGA CCTGGTGGGC AAAGATGTGG TCCGGCCATA CCAGACGATG 
TCTAACCCTC TTAGCAAGCT AACAGTACTC AACAGCCTAC ACTCACACTT CATTTTGGCT 
GATAATGGCA CCACCGGAAA GTACGGAGCA GAAGTCAAAC TGCGCAGGCA GCTGGAGAAG 
CACATCTCTC TGCAAAAGAT TAACACACGG ATTGGACAGG GTGTTCCAGT AGTGGCTCTG 
ATAGTGGAGG GGGGTCCGAA TGTCATCTCT ATCGTCCTGG AGTACCTAAG AGACACCCCT 
CCGGTGCCTG TTGTGGTGTG TGACGGCAGC GGTCGAGCCT CTGACATCCT GGCCTTTGGG 
CACAAGTACT CAGAGGAGGG CGGAATCATA AACGAATCTT TGAGAGACCA GTTATTAGTC 
ACCATTCAGA AGACGTTCAC ATACAGCCGG ACGCAGGCCC AGCATCTCTT CATCATTCTG 
ATGGAGTGCA TGAAGAAGAA AGAACTGATC ACAGTGTTTC GGATGGGATC AGAGGGACAC 
CAGGACATCG ATCTAGCCAT TCTAACAGCT CTTCTGAAAG GAGCGAATGC ATCAGCTCCA 
GATCAGCTAA GTCTGGCTTT GGCCTGGAAC AGAGTTGACA TCGCCCGGAG CCAGATCTTC 
ATTTACGGAC AGCAGTGGCC TGTTGGCTCT CTGGAGCAGT CTATGCTGGA CGCGCTGGTG 
CTGGACCGAG TGGACTTTGT AAAGCTGTTG ATTGAGAACG GAGTGAGCAT GCACCGTTTC 
CTGACACTCT CCAGACTTGA AGAGCTTTAT AATACGAGGC ACGGACCATC CAACACCTTG 
TACCACCTTG TCAGAGATGT GAAAAAGGGT AACCTGCCCC CCGACTACCG CATTAGCCTG 
ATTGACATCG GATTGGTTAT TGAATACCTC ATGGGTGGAG CCTATCGCTG CAATTACACC 
AGGAAGAGAT TTCGGACCCT CTATCACAAC CTCTTTGGAC CCAAAAGACC AAAAGCCCTG 
AAGCTGCTTG GAATGGAGGA TGACATGCCC ATTAGACGAG GGCGTCAGAA GACTACGCGG 
AAGCGAGAGG AGGAAGTAGA TATTGACCTG GATGACCCAG AAATCAATCA TTTCCCTTTC 
CCATTCCATG AGCTGATGGT TTGGGCTGTG CTGATGAAAC GGCAGAAGAT GGCACTGTTC 
TTCTGGCAGC ATGGAGAGGA GGCCATGGCT AAAGCGCTGG TGGCCTGCAA GCTCTGTAAG 
GCAATGGCTC ACGAAGCTTC TGAGAACGAC ATGGTCGATG ACATTTCACA GGAGCTCAAT 
CAAAACTCAA GGGAGTTTGG TCAGCTGGCT GTTGAGCTGC TCGATCAGTC CTATAAGCAG 
GATGAACAGA TGGCTATGAA GCTGTTGACC TACGAGCTGA AGAACTGGAG TAATGCCACC 
TGTCTGCAGC TGGCAGTGGC TGCCAAGCAC CGAGACTTCA TCGCCCACAC CTGTAGCCAG 
ATGCTGCTCA CAGACATGTG GATGGGACGT CTGCGCATGC GCAAGAACTC TGGCCTGAAG 
GTTATCTTGG GCCTGCTTCT CCCCCCTTCC ATATTGAGTC TGGAGTTTAA AAATAAAGAC 
GAGATGTCAT ACATGCCTCA GGATCAGGAC ACATATCTGC AGGAGAAGGA TGTGGATGAG 
CCCGAGAAAC AAGCTAAGGA GAAAGAGGAG GAGGACATGG AGTTCACAGC TATGTTGGGA 
AACGTCAGTT CAGAAGCATC GCGCAAGAAA CAGGTGGAGG AGGTTCAGAA CCGGCATCGG 
CTCATTCCAT TGGGTCGCAA AATATATGAG TTTTACAACG CCCCTATCGT CAAGTTCTGG 
TTTCACACGC TGGCGTATGT AGGATACTTG ATGCTCTTCA ACTATATTGT GCTGGTGAAG 
ATGGACCTGT GGCCTTCTCC TCAGGAGTGG ATTGTTATTG CCTACATATT CACCAATGGC 
ATCGAGAAGA TGAGAGAGAT CCTGATGTCC GAACCTGGGA AGCTGCTTCA GAAGGTGAAA 
GTGTGGTTAC AGGAGTACTG GAACATCACT GACCTCATGG CCATCCTCAT CTTCTCCATC 
GGGATGGTGC TTCGTCTGCA GGACCCGCCG CTGATGAGCT ACGGCAGGGT CATTTACTGC 
GTCAATATCA TTTATTGGTA CATACGACTC CTCGACATCT TCGGAGTGAA CAAATACCTG 
GGTCCATACG TCATGATGAT CGGCAAGATG ATGATTGACA TGATGTATTT TGTGATCATC 
ATGTTGGTGG TGCTGATGAG TTTCGGTGTG GCGCGTCAGG CCATCCTTAA TCCCAATGAA 
GACCCATCCT GGATGCTGGC ACGCAATATC TTCTTCATGC CATACTGGAT GATCTACGGA 
GAGGTGTTTG CTGACCAAAT CGACCATGTG AATAGTAGGA AGCTACATCC CAAAGTGAAT 
GACAAACTAT GGCTGGTCGA AAATTACATG CGCACTCAAG GAACTGGGCG TTACAGTGGT 
CCATCCCATA TAGCTCCTTG TGGTCAGAAC ATCACCACAG AAGAAGGAGT TGTCATGCCT 
CTTCCACCCT GCAAAACTGG AGCTTGGATT GTCCCTGCCA TAATGGCCTG CTACCTGCTG 
GTGGCCAACA TCCTGCTAGT TAACTTGCTC ATTGCTGTCT TTAATAACAC TTTCTTTGAA 
GTCAAGTCCA TTTCCAACCA GGTGTGGAAG TTTCAGAGGT ATCAGCTGAT CATGACCTTC 
CATGAACGCC CGGTCCTTCC TCCTCCACTC ATCATCTTCA GCCACATAAC TATGGTTCTC 
AAACACCTGT GCTGTCGCTG GAGGAAGCAC GATGAGGATG AACGGGACTA TGGGCTGAAG 
CTTTTCATCA CTGAAGATGA ACTGAAGAAA GTGCATGACT TTGAAGAGCA GTGCATGGAG 
GAGTATTTCA GAGAGAAAGA TGACCGCTTC AATTCCTCCA ATGACGAAAG GATACGTGTG 
ACATCTGAAA GGGTGGAGAA CATGGCGATG CGATTAGAGG AGGTGAATGA GAGGGAACAT 
TTTATGAAAG CGTCCTTGCA GACTGTAGAC ATCCGGCTCG CCCAGATGGA GGAGATGATC 
GGCCGCATTG CTGTGGCCCT AGAGAGAGTT GCTGGAATGG ACAGAGGCGA AGTTAACAAA 
GCCCGGTCCA GGACATCATC AGACTGCACT GATACCAATT ACATCCTGCG CCAAAGCAGT 
TTCAACAGCC AAGAAGGCAA TTCCTACAGG CTACAGGAGT CTCTGGAGCA GGGGGGCGAC 
GAGTCCATTT CTCCTACTTC CCCAACCGCC CTGGCTCCCC GTGTCCGCAG TCACTCCTTC 
TACGTGAGCC ACTCTTCCAA AGACAGGAGT GGGGCGGACC GTGGAGAGGG CTTCTTTAAA 
GACAAGTTGT TCAGCCTCCA CCGGGCCAAC AGCTCACAGT CTGTGTCCTC AGGAGCAGGT 
CCCAAAGAGT CCAAGCCAAC CCCTTTGAAT ACTTTATCCG TGCAGCAGCA GCTCCGCCCT 
TCTTCCTGCA TAGACATCTA TGTTTCTGCC TCAGAGGACG TCCCGCCCAC TGAGAGCTTC 
TTGGATTCGG TTCGGACAGT TCCAACTCTG GCTCGAGACT CATCTCTTCA TTCGGAGATC 
ATGGAGGCGG TGCTCTCAGG GGGACGGGAC TGTAGTGGAA GGGCAGGCGG CAGCGAAAGA 
CAGTCGGATG GTACAGTCCT GTTTGAGGAC AGCGCCGCCG CTGACCTATC GCTCTGCTCT 
GCCCACCTGC TGCCGGATTC CTTGCCCCCC TGGGACCTGG ACCCCTCACC GCCACCATCA 
GCTGGTGTAC TAGAGCGGTC CAAAAGTAGT CGCTTCCTGT CTACAGCAGG GCCTCTGTTT 
CTGGACGAGC CTCATTTGGT TAAGTCACAC AGTCTGATGT TCACATCACG TGGGTACTAT 
GGTGGCATGG GTGTGCAGGT GAAGGCGGCC GAATACACCA GCATCACAGA TTGCATCGAC 
ACCCGTTGCG TCTCAACCCC TTACCCTGTG CCAGAGCGGT CAGACTCACC TGGAGGATCT 
TTCACCTTCG ATAAGCCTCA AGACCTTGGC GTCTCGCACC CTGAGAGAGA CGCTGAGCTC 
AGCCACGCTG AGTCTGACTT AGAGGAGCCA GCAGAGGGTT CGGGAGACAC ATGTAAGGTG 
GGTCAGAGTA GCAGTAGTGG GGTAATAGGT GCAGACCTGG GCCTGGGCCT CGCACTAGGC 
CCGTTTTGCT CACCCATCTC TAGACTAGAG CGAGCAAACA GCTGCTCCTC TTCAGAGGAG 
TCGCACTCCA ACATCTACTC TCGAAAGAGC TTCTCAATAA GCGAGAGAAT GGACAAGGGC 
CGGGGGAGCT CCAGGAACCC TTTCCAGAAG GCCAGAACCG GGGCGAGGCT GGAGGGTAAG 
ACAGACTCAC TGTCTATGAG GAAAATGGCC AAACCGTCAG CTTTCAGAAG TTTTGACAGC 
AGACACAACT ACACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm3 ( XM_005171927.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171984.1 Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X6, mRNA.

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CloneID	ODa10542
Clone ID Related Accession (Same CDS sequence)	XM_005171927.3
Accession Version	XM_005171927.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transient receptor potential cation channel subfamily M member 3 isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171927.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGCC CTTTTCCGGA TGGCGGGAAC GTCCGGCCGG GAGCGATGAA CCAGCTGGAT 
GCTCCTCGGC CACTCAACTG GACCATACGG AAACTGTGCC ATGCAGCCTT CCTCCCGTCT 
GTGCGCCTGC TTAAGGCACA GAAATCATGG ATAGAAAGAG CGTTCAGCAA GAGAGAATGT 
GTGCACATTA TTGTAAGCAC CAAAGACCAC CATAGGTGCT GTTGTGGCCG TCTAATTGGT 
CAACATGTTG GCCTGCCCCC CAGCATCTCA TCCAATCAGA ATGAGAAGTC AGAACGCGTG 
CCAAAGAATG ACAGCTTGTC AGAGAAGTGG TCCATCAGCA AGCACACCCA GCTCAGTCCC 
ACCGACGCTT TTGGGACCAT CGAGTTCCAG GGAGGAGGAC ACTCTAATAA AGCAATGTAT 
GTACGAGTGT CTTACGACAC AAAACCAGAT TTACTGCTGC ATCTGATGAC AAAGGAGTGG 
CAACTGGAGC TTCCCAAGCT GCTCATCTCT GTTCACGGAG GACTTCAAAA CTTTGAGTTG 
CAGCCCAAAC TAAAGCAAGT CTTCGGCAAA GGTCTTATAA AGGCTGCAAT GACTACCGGA 
GCCTGGATCT TCACTGGAGG AGTTAATACT GGAGTCATCA GACATGTTGG TGATGCTCTG 
AAAGATCATG CTTCCAAATC AAGAGGGAAG ATCTGCACTA TCGGCATTGC CCCCTGGGGG 
ATTGTGGAGA ACCAGGAAGA CCTGGTGGGC AAAGATGTGG TCCGGCCATA CCAGACGATG 
TCTAACCCTC TTAGCAAGCT AACAGTACTC AACAGCCTAC ACTCACACTT CATTTTGGCT 
GATAATGGCA CCACCGGAAA GTACGGAGCA GAAGTCAAAC TGCGCAGGCA GCTGGAGAAG 
CACATCTCTC TGCAAAAGAT TAACACACGG ATTGGACAGG GTGTTCCAGT AGTGGCTCTG 
ATAGTGGAGG GGGGTCCGAA TGTCATCTCT ATCGTCCTGG AGTACCTAAG AGACACCCCT 
CCGGTGCCTG TTGTGGTGTG TGACGGCAGC GGTCGAGCCT CTGACATCCT GGCCTTTGGG 
CACAAGTACT CAGAGGAGGG CGGAATCATA AACGAATCTT TGAGAGACCA GTTATTAGTC 
ACCATTCAGA AGACGTTCAC ATACAGCCGG ACGCAGGCCC AGCATCTCTT CATCATTCTG 
ATGGAGTGCA TGAAGAAGAA AGAACTGATC ACAGTGTTTC GGATGGGATC AGAGGGACAC 
CAGGACATCG ATCTAGCCAT TCTAACAGCT CTTCTGAAAG GAGCGAATGC ATCAGCTCCA 
GATCAGCTAA GTCTGGCTTT GGCCTGGAAC AGAGTTGACA TCGCCCGGAG CCAGATCTTC 
ATTTACGGAC AGCAGTGGCC TGTTGGCTCT CTGGAGCAGT CTATGCTGGA CGCGCTGGTG 
CTGGACCGAG TGGACTTTGT AAAGCTGTTG ATTGAGAACG GAGTGAGCAT GCACCGTTTC 
CTGACACTCT CCAGACTTGA AGAGCTTTAT AATACGAGGC ACGGACCATC CAACACCTTG 
TACCACCTTG TCAGAGATGT GAAAAAGGGT AACCTGCCCC CCGACTACCG CATTAGCCTG 
ATTGACATCG GATTGGTTAT TGAATACCTC ATGGGTGGAG CCTATCGCTG CAATTACACC 
AGGAAGAGAT TTCGGACCCT CTATCACAAC CTCTTTGGAC CCAAAAGACC AAAAGCCCTG 
AAGCTGCTTG GAATGGAGGA TGACATGCCC ATTAGACGAG GGCGTCAGAA GACTACGCGG 
AAGCGAGAGG AGGAAGTAGA TATTGACCTG GATGACCCAG AAATCAATCA TTTCCCTTTC 
CCATTCCATG AGCTGATGGT TTGGGCTGTG CTGATGAAAC GGCAGAAGAT GGCACTGTTC 
TTCTGGCAGC ATGGAGAGGA GGCCATGGCT AAAGCGCTGG TGGCCTGCAA GCTCTGTAAG 
GCAATGGCTC ACGAAGCTTC TGAGAACGAC ATGGTCGATG ACATTTCACA GGAGCTCAAT 
CAAAACTCAA GGGAGTTTGG TCAGCTGGCT GTTGAGCTGC TCGATCAGTC CTATAAGCAG 
GATGAACAGA TGGCTATGAA GCTGTTGACC TACGAGCTGA AGAACTGGAG TAATGCCACC 
TGTCTGCAGC TGGCAGTGGC TGCCAAGCAC CGAGACTTCA TCGCCCACAC CTGTAGCCAG 
ATGCTGCTCA CAGACATGTG GATGGGACGT CTGCGCATGC GCAAGAACTC TGGCCTGAAG 
GTTATCTTGG GCCTGCTTCT CCCCCCTTCC ATATTGAGTC TGGAGTTTAA AAATAAAGAC 
GAGATGTCAT ACATGCCTCA GGATCAGGAC ACATATCTGC AGGAGAAGGA TGTGGATGAG 
CCCGAGAAAC AAGCTAAGGA GAAAGAGGAG GAGGACATGG AGTTCACAGT AAGATCTTAC 
TGTGAGACGC AGTACAACTC CGTGGCTATG TTGGGAAACG TCAGTTCAGA AGCATCGCGC 
AAGAAACAGG TGGAGGAGGT TCAGAACCGG CATCGGCTCA TTCCATTGGG TCGCAAAATA 
TATGAGTTTT ACAACGCCCC TATCGTCAAG TTCTGGTTTC ACACGCTGGC GTATGTAGGA 
TACTTGATGC TCTTCAACTA TATTGTGCTG GTGAAGATGG ACCTGTGGCC TTCTCCTCAG 
GAGTGGATTG TTATTGCCTA CATATTCACC AATGGCATCG AGAAGATGAG AGAGATCCTG 
ATGTCCGAAC CTGGGAAGCT GCTTCAGAAG GTGAAAGTGT GGTTACAGGA GTACTGGAAC 
ATCACTGACC TCATGGCCAT CCTCATCTTC TCCATCGGGA TGGTGCTTCG TCTGCAGGAC 
CCGCCGCTGA TGAGCTACGG CAGGGTCATT TACTGCGTCA ATATCATTTA TTGGTACATA 
CGACTCCTCG ACATCTTCGG AGTGAACAAA TACCTGGGTC CATACGTCAT GATGATCGGC 
AAGATGATGA TTGACATGAT GTATTTTGTG ATCATCATGT TGGTGGTGCT GATGAGTTTC 
GGTGTGGCGC GTCAGGCCAT CCTTAATCCC AATGAAGACC CATCCTGGAT GCTGGCACGC 
AATATCTTCT TCATGCCATA CTGGATGATC TACGGAGAGG TGTTTGCTGA CCAAATCGAC 
CCTCCTTGTG GTCAGAACAT CACCACAGAA GAAGGAGTTG TCATGCCTCT TCCACCCTGC 
AAAACTGGAG CTTGGATTGT CCCTGCCATA ATGGCCTGCT ACCTGCTGGT GGCCAACATC 
CTGCTAGTTA ACTTGCTCAT TGCTGTCTTT AATAACACTT TCTTTGAAGT CAAGTCCATT 
TCCAACCAGG TGTGGAAGTT TCAGAGGTAT CAGCTGATCA TGACCTTCCA TGAACGCCCG 
GTCCTTCCTC CTCCACTCAT CATCTTCAGC CACATAACTA TGGTTCTCAA ACACCTGTGC 
TGTCGCTGGA GGAAGCACGA TGAGGATGAA CGGGACTATG GGCTGAAGCT TTTCATCACT 
GAAGATGAAC TGAAGAAAGT GCATGACTTT GAAGAGCAGT GCATGGAGGA GTATTTCAGA 
GAGAAAGATG ACCGCTTCAA TTCCTCCAAT GACGAAAGGA TACGTGTGAC ATCTGAAAGG 
GTGGAGAACA TGGCGATGCG ATTAGAGGAG GTGAATGAGA GGGAACATTT TATGAAAGCG 
TCCTTGCAGA CTGTAGACAT CCGGCTCGCC CAGATGGAGG AGATGATCGG CCGCATTGCT 
GTGGCCCTAG AGAGAGTTGC TGGAATGGAC AGAGGCGAAG TTAACAAAGC CCGGTCCAGG 
ACATCATCAG ACTGCACTGA TACCAATTAC ATCCTGCGCC AAAGCAGTTT CAACAGCCAA 
GAAGGCAATT CCTACAGGCT ACAGGAGTCT CTGGAGCAGG GGGGCGACGA GTCCATTTCT 
CCTACTTCCC CAACCGCCCT GGCTCCCCGT GTCCGCAGTC ACTCCTTCTA CGTGAGCCAC 
TCTTCCAAAG ACAGGAGTGG GGCGGACCGT GGAGAGGGCT TCTTTAAAGA CAAGTTGTTC 
AGCCTCCACC GGGCCAACAG CTCACAGTCT GTGTCCTCAG GAGCAGGTCC CAAAGAGTCC 
AAGCCAACCC CTTTGAATAC TTTATCCGTG CAGCAGCAGC TCCGCCCTTC TTCCTGCATA 
GACATCTATG TTTCTGCCTC AGAGGACGTC CCGCCCACTG AGAGCTTCTT GGATTCGGTT 
CGGACAGTTC CAACTCTGGC TCGAGACTCA TCTCTTCATT CGGAGATCAT GGAGGCGGTG 
CTCTCAGGGG GACGGGACTG TAGTGGAAGG GCAGGCGGCA GCGAAAGACA GTCGGATGGT 
ACAGTCCTGT TTGAGGACAG CGCCGCCGCT GACCTATCGC TCTGCTCTGC CCACCTGCTG 
CCGGATTCCT TGCCCCCCTG GGACCTGGAC CCCTCACCGC CACCATCAGC TGGTGTACTA 
GAGCGGTCCA AAAGTAGTCG CTTCCTGTCT ACAGCAGGGC CTCTGTTTCT GGACGAGCCT 
CATTTGGTTA AGTCACACAG TCTGATGTTC ACATCACGTG GGTACTATGG TGGCATGGGT 
GTGCAGGTGA AGGCGGCCGA ATACACCAGC ATCACAGATT GCATCGACAC CCGTTGCGTC 
TCAACCCCTT ACCCTGTGCC AGAGCGGTCA GACTCACCTG GAGGATCTTT CACCTTCGAT 
AAGCCTCAAG ACCTTGGCGT CTCGCACCCT GAGAGAGACG CTGAGCTCAG CCACGCTGAG 
TCTGACTTAG AGGAGCCAGC AGAGGGTTCG GGAGACACAT GTAAGGTGGG TCAGAGTAGC 
AGTAGTGGGG TAATAGGTGC AGACCTGGGC CTGGGCCTCG CACTAGGCCC GTTTTGCTCA 
CCCATCTCTA GACTAGAGCG AGCAAACAGC TGCTCCTCTT CAGAGGAGTC GCACTCCAAC 
ATCTACTCTC GAAAGAGCTT CTCAATAAGC GAGAGAATGG ACAAGGGCCG GGGGAGCTCC 
AGGAACCCTT TCCAGAAGGC CAGAACCGGG GCGAGGCTGG AGGGTAAGAC AGACTCACTG 
TCTATGAGGA AAATGGCCAA ACCGTCAGCT TTCAGAAGTT TTGACAGCAG ACACAACTAC 
ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171984.1
CDS	375..5540
Translation	MWGPFPDGGNVRPGAMNQLDAPRPLNWTIRKLCHAAFLPSVRLLKAQKSWIERAFSKRECVHIIVSTKDHHRCCCGRLIGQHVGLPPSISSNQNEKSERVPKNDSLSEKWSISKHTQLSPTDAFGTIEFQGGGHSNKAMYVRVSYDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLVGKDVVRPYQTMSNPLSKLTVLNSLHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGIINESLRDQLLVTIQKTFTYSRTQAQHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQSMLDALVLDRVDFVKLLIENGVSMHRFLTLSRLEELYNTRHGPSNTLYHLVRDVKKGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDMPIRRGRQKTTRKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNQNSREFGQLAVELLDQSYKQDEQMAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGLLLPPSILSLEFKNKDEMSYMPQDQDTYLQEKDVDEPEKQAKEKEEEDMEFTVRSYCETQYNSVAMLGNVSSEASRKKQVEEVQNRHRLIPLGRKIYEFYNAPIVKFWFHTLAYVGYLMLFNYIVLVKMDLWPSPQEWIVIAYIFTNGIEKMREILMSEPGKLLQKVKVWLQEYWNITDLMAILIFSIGMVLRLQDPPLMSYGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDPPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHITMVLKHLCCRWRKHDEDERDYGLKLFITEDELKKVHDFEEQCMEEYFREKDDRFNSSNDERIRVTSERVENMAMRLEEVNEREHFMKASLQTVDIRLAQMEEMIGRIAVALERVAGMDRGEVNKARSRTSSDCTDTNYILRQSSFNSQEGNSYRLQESLEQGGDESISPTSPTALAPRVRSHSFYVSHSSKDRSGADRGEGFFKDKLFSLHRANSSQSVSSGAGPKESKPTPLNTLSVQQQLRPSSCIDIYVSASEDVPPTESFLDSVRTVPTLARDSSLHSEIMEAVLSGGRDCSGRAGGSERQSDGTVLFEDSAAADLSLCSAHLLPDSLPPWDLDPSPPPSAGVLERSKSSRFLSTAGPLFLDEPHLVKSHSLMFTSRGYYGGMGVQVKAAEYTSITDCIDTRCVSTPYPVPERSDSPGGSFTFDKPQDLGVSHPERDAELSHAESDLEEPAEGSGDTCKVGQSSSSGVIGADLGLGLALGPFCSPISRLERANSCSSSEESHSNIYSRKSFSISERMDKGRGSSRNPFQKARTGARLEGKTDSLSMRKMAKPSAFRSFDSRHNYT

Target ORF information:

RefSeq Version	XM_005171927.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171927.3

ORF Insert Sequence:

ATGTGGGGCC CTTTTCCGGA TGGCGGGAAC GTCCGGCCGG GAGCGATGAA CCAGCTGGAT 
GCTCCTCGGC CACTCAACTG GACCATACGG AAACTGTGCC ATGCAGCCTT CCTCCCGTCT 
GTGCGCCTGC TTAAGGCACA GAAATCATGG ATAGAAAGAG CGTTCAGCAA GAGAGAATGT 
GTGCACATTA TTGTAAGCAC CAAAGACCAC CATAGGTGCT GTTGTGGCCG TCTAATTGGT 
CAACATGTTG GCCTGCCCCC CAGCATCTCA TCCAATCAGA ATGAGAAGTC AGAACGCGTG 
CCAAAGAATG ACAGCTTGTC AGAGAAGTGG TCCATCAGCA AGCACACCCA GCTCAGTCCC 
ACCGACGCTT TTGGGACCAT CGAGTTCCAG GGAGGAGGAC ACTCTAATAA AGCAATGTAT 
GTACGAGTGT CTTACGACAC AAAACCAGAT TTACTGCTGC ATCTGATGAC AAAGGAGTGG 
CAACTGGAGC TTCCCAAGCT GCTCATCTCT GTTCACGGAG GACTTCAAAA CTTTGAGTTG 
CAGCCCAAAC TAAAGCAAGT CTTCGGCAAA GGTCTTATAA AGGCTGCAAT GACTACCGGA 
GCCTGGATCT TCACTGGAGG AGTTAATACT GGAGTCATCA GACATGTTGG TGATGCTCTG 
AAAGATCATG CTTCCAAATC AAGAGGGAAG ATCTGCACTA TCGGCATTGC CCCCTGGGGG 
ATTGTGGAGA ACCAGGAAGA CCTGGTGGGC AAAGATGTGG TCCGGCCATA CCAGACGATG 
TCTAACCCTC TTAGCAAGCT AACAGTACTC AACAGCCTAC ACTCACACTT CATTTTGGCT 
GATAATGGCA CCACCGGAAA GTACGGAGCA GAAGTCAAAC TGCGCAGGCA GCTGGAGAAG 
CACATCTCTC TGCAAAAGAT TAACACACGG ATTGGACAGG GTGTTCCAGT AGTGGCTCTG 
ATAGTGGAGG GGGGTCCGAA TGTCATCTCT ATCGTCCTGG AGTACCTAAG AGACACCCCT 
CCGGTGCCTG TTGTGGTGTG TGACGGCAGC GGTCGAGCCT CTGACATCCT GGCCTTTGGG 
CACAAGTACT CAGAGGAGGG CGGAATCATA AACGAATCTT TGAGAGACCA GTTATTAGTC 
ACCATTCAGA AGACGTTCAC ATACAGCCGG ACGCAGGCCC AGCATCTCTT CATCATTCTG 
ATGGAGTGCA TGAAGAAGAA AGAACTGATC ACAGTGTTTC GGATGGGATC AGAGGGACAC 
CAGGACATCG ATCTAGCCAT TCTAACAGCT CTTCTGAAAG GAGCGAATGC ATCAGCTCCA 
GATCAGCTAA GTCTGGCTTT GGCCTGGAAC AGAGTTGACA TCGCCCGGAG CCAGATCTTC 
ATTTACGGAC AGCAGTGGCC TGTTGGCTCT CTGGAGCAGT CTATGCTGGA CGCGCTGGTG 
CTGGACCGAG TGGACTTTGT AAAGCTGTTG ATTGAGAACG GAGTGAGCAT GCACCGTTTC 
CTGACACTCT CCAGACTTGA AGAGCTTTAT AATACGAGGC ACGGACCATC CAACACCTTG 
TACCACCTTG TCAGAGATGT GAAAAAGGGT AACCTGCCCC CCGACTACCG CATTAGCCTG 
ATTGACATCG GATTGGTTAT TGAATACCTC ATGGGTGGAG CCTATCGCTG CAATTACACC 
AGGAAGAGAT TTCGGACCCT CTATCACAAC CTCTTTGGAC CCAAAAGACC AAAAGCCCTG 
AAGCTGCTTG GAATGGAGGA TGACATGCCC ATTAGACGAG GGCGTCAGAA GACTACGCGG 
AAGCGAGAGG AGGAAGTAGA TATTGACCTG GATGACCCAG AAATCAATCA TTTCCCTTTC 
CCATTCCATG AGCTGATGGT TTGGGCTGTG CTGATGAAAC GGCAGAAGAT GGCACTGTTC 
TTCTGGCAGC ATGGAGAGGA GGCCATGGCT AAAGCGCTGG TGGCCTGCAA GCTCTGTAAG 
GCAATGGCTC ACGAAGCTTC TGAGAACGAC ATGGTCGATG ACATTTCACA GGAGCTCAAT 
CAAAACTCAA GGGAGTTTGG TCAGCTGGCT GTTGAGCTGC TCGATCAGTC CTATAAGCAG 
GATGAACAGA TGGCTATGAA GCTGTTGACC TACGAGCTGA AGAACTGGAG TAATGCCACC 
TGTCTGCAGC TGGCAGTGGC TGCCAAGCAC CGAGACTTCA TCGCCCACAC CTGTAGCCAG 
ATGCTGCTCA CAGACATGTG GATGGGACGT CTGCGCATGC GCAAGAACTC TGGCCTGAAG 
GTTATCTTGG GCCTGCTTCT CCCCCCTTCC ATATTGAGTC TGGAGTTTAA AAATAAAGAC 
GAGATGTCAT ACATGCCTCA GGATCAGGAC ACATATCTGC AGGAGAAGGA TGTGGATGAG 
CCCGAGAAAC AAGCTAAGGA GAAAGAGGAG GAGGACATGG AGTTCACAGT AAGATCTTAC 
TGTGAGACGC AGTACAACTC CGTGGCTATG TTGGGAAACG TCAGTTCAGA AGCATCGCGC 
AAGAAACAGG TGGAGGAGGT TCAGAACCGG CATCGGCTCA TTCCATTGGG TCGCAAAATA 
TATGAGTTTT ACAACGCCCC TATCGTCAAG TTCTGGTTTC ACACGCTGGC GTATGTAGGA 
TACTTGATGC TCTTCAACTA TATTGTGCTG GTGAAGATGG ACCTGTGGCC TTCTCCTCAG 
GAGTGGATTG TTATTGCCTA CATATTCACC AATGGCATCG AGAAGATGAG AGAGATCCTG 
ATGTCCGAAC CTGGGAAGCT GCTTCAGAAG GTGAAAGTGT GGTTACAGGA GTACTGGAAC 
ATCACTGACC TCATGGCCAT CCTCATCTTC TCCATCGGGA TGGTGCTTCG TCTGCAGGAC 
CCGCCGCTGA TGAGCTACGG CAGGGTCATT TACTGCGTCA ATATCATTTA TTGGTACATA 
CGACTCCTCG ACATCTTCGG AGTGAACAAA TACCTGGGTC CATACGTCAT GATGATCGGC 
AAGATGATGA TTGACATGAT GTATTTTGTG ATCATCATGT TGGTGGTGCT GATGAGTTTC 
GGTGTGGCGC GTCAGGCCAT CCTTAATCCC AATGAAGACC CATCCTGGAT GCTGGCACGC 
AATATCTTCT TCATGCCATA CTGGATGATC TACGGAGAGG TGTTTGCTGA CCAAATCGAC 
CCTCCTTGTG GTCAGAACAT CACCACAGAA GAAGGAGTTG TCATGCCTCT TCCACCCTGC 
AAAACTGGAG CTTGGATTGT CCCTGCCATA ATGGCCTGCT ACCTGCTGGT GGCCAACATC 
CTGCTAGTTA ACTTGCTCAT TGCTGTCTTT AATAACACTT TCTTTGAAGT CAAGTCCATT 
TCCAACCAGG TGTGGAAGTT TCAGAGGTAT CAGCTGATCA TGACCTTCCA TGAACGCCCG 
GTCCTTCCTC CTCCACTCAT CATCTTCAGC CACATAACTA TGGTTCTCAA ACACCTGTGC 
TGTCGCTGGA GGAAGCACGA TGAGGATGAA CGGGACTATG GGCTGAAGCT TTTCATCACT 
GAAGATGAAC TGAAGAAAGT GCATGACTTT GAAGAGCAGT GCATGGAGGA GTATTTCAGA 
GAGAAAGATG ACCGCTTCAA TTCCTCCAAT GACGAAAGGA TACGTGTGAC ATCTGAAAGG 
GTGGAGAACA TGGCGATGCG ATTAGAGGAG GTGAATGAGA GGGAACATTT TATGAAAGCG 
TCCTTGCAGA CTGTAGACAT CCGGCTCGCC CAGATGGAGG AGATGATCGG CCGCATTGCT 
GTGGCCCTAG AGAGAGTTGC TGGAATGGAC AGAGGCGAAG TTAACAAAGC CCGGTCCAGG 
ACATCATCAG ACTGCACTGA TACCAATTAC ATCCTGCGCC AAAGCAGTTT CAACAGCCAA 
GAAGGCAATT CCTACAGGCT ACAGGAGTCT CTGGAGCAGG GGGGCGACGA GTCCATTTCT 
CCTACTTCCC CAACCGCCCT GGCTCCCCGT GTCCGCAGTC ACTCCTTCTA CGTGAGCCAC 
TCTTCCAAAG ACAGGAGTGG GGCGGACCGT GGAGAGGGCT TCTTTAAAGA CAAGTTGTTC 
AGCCTCCACC GGGCCAACAG CTCACAGTCT GTGTCCTCAG GAGCAGGTCC CAAAGAGTCC 
AAGCCAACCC CTTTGAATAC TTTATCCGTG CAGCAGCAGC TCCGCCCTTC TTCCTGCATA 
GACATCTATG TTTCTGCCTC AGAGGACGTC CCGCCCACTG AGAGCTTCTT GGATTCGGTT 
CGGACAGTTC CAACTCTGGC TCGAGACTCA TCTCTTCATT CGGAGATCAT GGAGGCGGTG 
CTCTCAGGGG GACGGGACTG TAGTGGAAGG GCAGGCGGCA GCGAAAGACA GTCGGATGGT 
ACAGTCCTGT TTGAGGACAG CGCCGCCGCT GACCTATCGC TCTGCTCTGC CCACCTGCTG 
CCGGATTCCT TGCCCCCCTG GGACCTGGAC CCCTCACCGC CACCATCAGC TGGTGTACTA 
GAGCGGTCCA AAAGTAGTCG CTTCCTGTCT ACAGCAGGGC CTCTGTTTCT GGACGAGCCT 
CATTTGGTTA AGTCACACAG TCTGATGTTC ACATCACGTG GGTACTATGG TGGCATGGGT 
GTGCAGGTGA AGGCGGCCGA ATACACCAGC ATCACAGATT GCATCGACAC CCGTTGCGTC 
TCAACCCCTT ACCCTGTGCC AGAGCGGTCA GACTCACCTG GAGGATCTTT CACCTTCGAT 
AAGCCTCAAG ACCTTGGCGT CTCGCACCCT GAGAGAGACG CTGAGCTCAG CCACGCTGAG 
TCTGACTTAG AGGAGCCAGC AGAGGGTTCG GGAGACACAT GTAAGGTGGG TCAGAGTAGC 
AGTAGTGGGG TAATAGGTGC AGACCTGGGC CTGGGCCTCG CACTAGGCCC GTTTTGCTCA 
CCCATCTCTA GACTAGAGCG AGCAAACAGC TGCTCCTCTT CAGAGGAGTC GCACTCCAAC 
ATCTACTCTC GAAAGAGCTT CTCAATAAGC GAGAGAATGG ACAAGGGCCG GGGGAGCTCC 
AGGAACCCTT TCCAGAAGGC CAGAACCGGG GCGAGGCTGG AGGGTAAGAC AGACTCACTG 
TCTATGAGGA AAATGGCCAA ACCGTCAGCT TTCAGAAGTT TTGACAGCAG ACACAACTAC 
ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm3 ( XM_005171928.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171985.1 Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X12, mRNA.

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CloneID	ODa10543
Clone ID Related Accession (Same CDS sequence)	XM_005171928.4 , XM_005171928.3
Accession Version	XM_005171928.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily M member 3 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171928.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAAAA GAAAATGGGA GGGGAGGAGA GAAGGCTTCA AGGTGAGCTC CACGAGGGAG 
GAGGCACAGG GGCCTGGACG CCCCTCCACC AGAGGGCGCT TCTCCGAATC TTGGAAGAGA 
CTGAGCTCCA GACAGGGGTC TACCAAGAGG TCAGGACTGG CCTCCCAGCA AGCTGCGGCA 
CAGAAATCAT GGATAGAAAG AGCGTTCAGC AAGAGAGAAT GTGTGCACAT TATTGTAAGC 
ACCAAAGACC ACCATAGGTG CTGTTGTGGC CGTCTAATTG GTCAACATGT TGGCCTGCCC 
CCCAGCATCT CATCCAATCA GAATGAGAAG TCAGAACGCG TGCCAAAGAA TGACAGCTTG 
TCAGAGAAGT GGTCCATCAG CAAGCACACC CAGCTCAGTC CCACCGACGC TTTTGGGACC 
ATCGAGTTCC AGGGAGGAGG ACACTCTAAT AAAGCAATGT ATGTACGAGT GTCTTACGAC 
ACAAAACCAG ATTTACTGCT GCATCTGATG ACAAAGGAGT GGCAACTGGA GCTTCCCAAG 
CTGCTCATCT CTGTTCACGG AGGACTTCAA AACTTTGAGT TGCAGCCCAA ACTAAAGCAA 
GTCTTCGGCA AAGGTCTTAT AAAGGCTGCA ATGACTACCG GAGCCTGGAT CTTCACTGGA 
GGAGTTAATA CTGGAGTCAT CAGACATGTT GGTGATGCTC TGAAAGATCA TGCTTCCAAA 
TCAAGAGGGA AGATCTGCAC TATCGGCATT GCCCCCTGGG GGATTGTGGA GAACCAGGAA 
GACCTGGTGG GCAAAGATGT GGTCCGGCCA TACCAGACGA TGTCTAACCC TCTTAGCAAG 
CTAACAGTAC TCAACAGCCT ACACTCACAC TTCATTTTGG CTGATAATGG CACCACCGGA 
AAGTACGGAG CAGAAGTCAA ACTGCGCAGG CAGCTGGAGA AGCACATCTC TCTGCAAAAG 
ATTAACACAC GGATTGGACA GGGTGTTCCA GTAGTGGCTC TGATAGTGGA GGGGGGTCCG 
AATGTCATCT CTATCGTCCT GGAGTACCTA AGAGACACCC CTCCGGTGCC TGTTGTGGTG 
TGTGACGGCA GCGGTCGAGC CTCTGACATC CTGGCCTTTG GGCACAAGTA CTCAGAGGAG 
GGCGGAATCA TAAACGAATC TTTGAGAGAC CAGTTATTAG TCACCATTCA GAAGACGTTC 
ACATACAGCC GGACGCAGGC CCAGCATCTC TTCATCATTC TGATGGAGTG CATGAAGAAG 
AAAGAACTGA TCACAGTGTT TCGGATGGGA TCAGAGGGAC ACCAGGACAT CGATCTAGCC 
ATTCTAACAG CTCTTCTGAA AGGAGCGAAT GCATCAGCTC CAGATCAGCT AAGTCTGGCT 
TTGGCCTGGA ACAGAGTTGA CATCGCCCGG AGCCAGATCT TCATTTACGG ACAGCAGTGG 
CCTGTTGGCT CTCTGGAGCA GTCTATGCTG GACGCGCTGG TGCTGGACCG AGTGGACTTT 
GTAAAGCTGT TGATTGAGAA CGGAGTGAGC ATGCACCGTT TCCTGACACT CTCCAGACTT 
GAAGAGCTTT ATAATACGAG GCACGGACCA TCCAACACCT TGTACCACCT TGTCAGAGAT 
GTGAAAAAGG GTAACCTGCC CCCCGACTAC CGCATTAGCC TGATTGACAT CGGATTGGTT 
ATTGAATACC TCATGGGTGG AGCCTATCGC TGCAATTACA CCAGGAAGAG ATTTCGGACC 
CTCTATCACA ACCTCTTTGG ACCCAAAAGA CCAAAAGCCC TGAAGCTGCT TGGAATGGAG 
GATGACATGC CCATTAGACG AGGGCGTCAG AAGACTACGC GGAAGCGAGA GGAGGAAGTA 
GATATTGACC TGGATGACCC AGAAATCAAT CATTTCCCTT TCCCATTCCA TGAGCTGATG 
GTTTGGGCTG TGCTGATGAA ACGGCAGAAG ATGGCACTGT TCTTCTGGCA GCATGGAGAG 
GAGGCCATGG CTAAAGCGCT GGTGGCCTGC AAGCTCTGTA AGGCAATGGC TCACGAAGCT 
TCTGAGAACG ACATGGTCGA TGACATTTCA CAGGAGCTCA ATCAAAACTC AAGGGAGTTT 
GGTCAGCTGG CTGTTGAGCT GCTCGATCAG TCCTATAAGC AGGATGAACA GATGGCTATG 
AAGCTGTTGA CCTACGAGCT GAAGAACTGG AGTAATGCCA CCTGTCTGCA GCTGGCAGTG 
GCTGCCAAGC ACCGAGACTT CATCGCCCAC ACCTGTAGCC AGATGCTGCT CACAGACATG 
TGGATGGGAC GTCTGCGCAT GCGCAAGAAC TCTGGCCTGA AGGTTATCTT GGGCCTGCTT 
CTCCCCCCTT CCATATTGAG TCTGGAGTTT AAAAATAAAG ACGAGATGTC ATACATGCCT 
CAGGATCAGG ACACATATCT GCAGGAGAAG GATGTGGATG AGCCCGAGAA ACAAGCTAAG 
GAGAAAGAGG AGGAGGACAT GGAGTTCACA GCTATGTTGG GAAACGTCAG TTCAGAAGCA 
TCGCGCAAGA AACAGGTGGA GGAGGTTCAG AACCGGCATC GGCTCATTCC ATTGGGTCGC 
AAAATATATG AGTTTTACAA CGCCCCTATC GTCAAGTTCT GGTTTCACAC GCTGGCGTAT 
GTAGGATACT TGATGCTCTT CAACTATATT GTGCTGGTGA AGATGGACCT GTGGCCTTCT 
CCTCAGGAGT GGATTGTTAT TGCCTACATA TTCACCAATG GCATCGAGAA GATGAGAGAG 
ATCCTGATGT CCGAACCTGG GAAGCTGCTT CAGAAGGTGA AAGTGTGGTT ACAGGAGTAC 
TGGAACATCA CTGACCTCAT GGCCATCCTC ATCTTCTCCA TCGGGATGGT GCTTCGTCTG 
CAGGACCCGC CGCTGATGAG CTACGGCAGG GTCATTTACT GCGTCAATAT CATTTATTGG 
TACATACGAC TCCTCGACAT CTTCGGAGTG AACAAATACC TGGGTCCATA CGTCATGATG 
ATCGGCAAGA TGATGATTGA CATGATGTAT TTTGTGATCA TCATGTTGGT GGTGCTGATG 
AGTTTCGGTG TGGCGCGTCA GGCCATCCTT AATCCCAATG AAGACCCATC CTGGATGCTG 
GCACGCAATA TCTTCTTCAT GCCATACTGG ATGATCTACG GAGAGGTGTT TGCTGACCAA 
ATCGACCCTC CTTGTGGTCA GAACATCACC ACAGAAGAAG GAGTTGTCAT GCCTCTTCCA 
CCCTGCAAAA CTGGAGCTTG GATTGTCCCT GCCATAATGG CCTGCTACCT GCTGGTGGCC 
AACATCCTGC TAGTTAACTT GCTCATTGCT GTCTTTAATA ACACTTTCTT TGAAGTCAAG 
TCCATTTCCA ACCAGGTGTG GAAGTTTCAG AGGTATCAGC TGATCATGAC CTTCCATGAA 
CGCCCGGTCC TTCCTCCTCC ACTCATCATC TTCAGCCACA TAACTATGGT TCTCAAACAC 
CTGTGCTGTC GCTGGAGGAA GCACGATGAG GATGAACGGG ACTATGGGCT GAAGCTTTTC 
ATCACTGAAG ATGAACTGAA GAAAGTGCAT GACTTTGAAG AGCAGTGCAT GGAGGAGTAT 
TTCAGAGAGA AAGATGACCG CTTCAATTCC TCCAATGACG AAAGGATACG TGTGACATCT 
GAAAGGGTGG AGAACATGGC GATGCGATTA GAGGAGGTGA ATGAGAGGGA ACATTTTATG 
AAAGCGTCCT TGCAGACTGT AGACATCCGG CTCGCCCAGA TGGAGGAGAT GATCGGCCGC 
ATTGCTGTGG CCCTAGAGAG AGTTGCTGGA ATGGACAGAG GCGAAGTTAA CAAAGCCCGG 
TCCAGGACAT CATCAGACTG CACTGATACC AATTACATCC TGCGCCAAAG CAGTTTCAAC 
AGCCAAGAAG GCAATTCCTA CAGGCTACAG GAGTCTCTGG AGCAGGGGGG CGACGAGTCC 
ATTTCTCCTA CTTCCCCAAC CGCCCTGGCT CCCCGTGTCC GCAGTCACTC CTTCTACGTG 
AGCCACTCTT CCAAAGACAG GAGTGGGGCG GACCGTGGAG AGGGCTTCTT TAAAGACAAG 
TTGTTCAGCC TCCACCGGGC CAACAGCTCA CAGTCTGTGT CCTCAGGAGC AGGTCCCAAA 
GAGTCCAAGC CAACCCCTTT GAATACTTTA TCCGTGCAGC AGCAGCTCCG CCCTTCTTCC 
TGCATAGACA TCTATGTTTC TGCCTCAGAG GACGTCCCGC CCACTGAGAG CTTCTTGGAT 
TCGGTTCGGA CAGTTCCAAC TCTGGCTCGA GACTCATCTC TTCATTCGGA GATCATGGAG 
GCGGTGCTCT CAGGGGGACG GGACTGTAGT GGAAGGGCAG GCGGCAGCGA AAGACAGTCG 
GATGGTACAG TCCTGTTTGA GGACAGCGCC GCCGCTGACC TATCGCTCTG CTCTGCCCAC 
CTGCTGCCGG ATTCCTTGCC CCCCTGGGAC CTGGACCCCT CACCGCCACC ATCAGCTGGT 
GTACTAGAGC GGTCCAAAAG TAGTCGCTTC CTGTCTACAG CAGGGCCTCT GTTTCTGGAC 
GAGCCTCATT TGGTTAAGTC ACACAGTCTG ATGTTCACAT CACGTGGGTA CTATGGTGGC 
ATGGGTGTGC AGGTGAAGGC GGCCGAATAC ACCAGCATCA CAGATTGCAT CGACACCCGT 
TGCGTCTCAA CCCCTTACCC TGTGCCAGAG CGGTCAGACT CACCTGGAGG ATCTTTCACC 
TTCGATAAGC CTCAAGACCT TGGCGTCTCG CACCCTGAGA GAGACGCTGA GCTCAGCCAC 
GCTGAGTCTG ACTTAGAGGA GCCAGCAGAG GGTTCGGGAG ACACATGTAA GGTGGGTCAG 
AGTAGCAGTA GTGGGGTAAT AGGTGCAGAC CTGGGCCTGG GCCTCGCACT AGGCCCGTTT 
TGCTCACCCA TCTCTAGACT AGAGCGAGCA AACAGCTGCT CCTCTTCAGA GGAGTCGCAC 
TCCAACATCT ACTCTCGAAA GAGCTTCTCA ATAAGCGAGA GAATGGACAA GGGCCGGGGG 
AGCTCCAGGA ACCCTTTCCA GAAGGCCAGA ACCGGGGCGA GGCTGGAGGG TAAGACAGAC 
TCACTGTCTA TGAGGAAAAT GGCCAAACCG TCAGCTTTCA GAAGTTTTGA CAGCAGACAC 
AACTACACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171985.1
CDS	378..5549
Translation	MTKRKWEGRREGFKVSSTREEAQGPGRPSTRGRFSESWKRLSSRQGSTKRSGLASQQAAAQKSWIERAFSKRECVHIIVSTKDHHRCCCGRLIGQHVGLPPSISSNQNEKSERVPKNDSLSEKWSISKHTQLSPTDAFGTIEFQGGGHSNKAMYVRVSYDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLVGKDVVRPYQTMSNPLSKLTVLNSLHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGIINESLRDQLLVTIQKTFTYSRTQAQHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQSMLDALVLDRVDFVKLLIENGVSMHRFLTLSRLEELYNTRHGPSNTLYHLVRDVKKGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDMPIRRGRQKTTRKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNQNSREFGQLAVELLDQSYKQDEQMAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGLLLPPSILSLEFKNKDEMSYMPQDQDTYLQEKDVDEPEKQAKEKEEEDMEFTAMLGNVSSEASRKKQVEEVQNRHRLIPLGRKIYEFYNAPIVKFWFHTLAYVGYLMLFNYIVLVKMDLWPSPQEWIVIAYIFTNGIEKMREILMSEPGKLLQKVKVWLQEYWNITDLMAILIFSIGMVLRLQDPPLMSYGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDPPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHITMVLKHLCCRWRKHDEDERDYGLKLFITEDELKKVHDFEEQCMEEYFREKDDRFNSSNDERIRVTSERVENMAMRLEEVNEREHFMKASLQTVDIRLAQMEEMIGRIAVALERVAGMDRGEVNKARSRTSSDCTDTNYILRQSSFNSQEGNSYRLQESLEQGGDESISPTSPTALAPRVRSHSFYVSHSSKDRSGADRGEGFFKDKLFSLHRANSSQSVSSGAGPKESKPTPLNTLSVQQQLRPSSCIDIYVSASEDVPPTESFLDSVRTVPTLARDSSLHSEIMEAVLSGGRDCSGRAGGSERQSDGTVLFEDSAAADLSLCSAHLLPDSLPPWDLDPSPPPSAGVLERSKSSRFLSTAGPLFLDEPHLVKSHSLMFTSRGYYGGMGVQVKAAEYTSITDCIDTRCVSTPYPVPERSDSPGGSFTFDKPQDLGVSHPERDAELSHAESDLEEPAEGSGDTCKVGQSSSSGVIGADLGLGLALGPFCSPISRLERANSCSSSEESHSNIYSRKSFSISERMDKGRGSSRNPFQKARTGARLEGKTDSLSMRKMAKPSAFRSFDSRHNYT

Target ORF information:

RefSeq Version	XM_005171928.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171928.4

ORF Insert Sequence:

ATGACAAAAA GAAAATGGGA GGGGAGGAGA GAAGGCTTCA AGGTGAGCTC CACGAGGGAG 
GAGGCACAGG GGCCTGGACG CCCCTCCACC AGAGGGCGCT TCTCCGAATC TTGGAAGAGA 
CTGAGCTCCA GACAGGGGTC TACCAAGAGG TCAGGACTGG CCTCCCAGCA AGCTGCGGCA 
CAGAAATCAT GGATAGAAAG AGCGTTCAGC AAGAGAGAAT GTGTGCACAT TATTGTAAGC 
ACCAAAGACC ACCATAGGTG CTGTTGTGGC CGTCTAATTG GTCAACATGT TGGCCTGCCC 
CCCAGCATCT CATCCAATCA GAATGAGAAG TCAGAACGCG TGCCAAAGAA TGACAGCTTG 
TCAGAGAAGT GGTCCATCAG CAAGCACACC CAGCTCAGTC CCACCGACGC TTTTGGGACC 
ATCGAGTTCC AGGGAGGAGG ACACTCTAAT AAAGCAATGT ATGTACGAGT GTCTTACGAC 
ACAAAACCAG ATTTACTGCT GCATCTGATG ACAAAGGAGT GGCAACTGGA GCTTCCCAAG 
CTGCTCATCT CTGTTCACGG AGGACTTCAA AACTTTGAGT TGCAGCCCAA ACTAAAGCAA 
GTCTTCGGCA AAGGTCTTAT AAAGGCTGCA ATGACTACCG GAGCCTGGAT CTTCACTGGA 
GGAGTTAATA CTGGAGTCAT CAGACATGTT GGTGATGCTC TGAAAGATCA TGCTTCCAAA 
TCAAGAGGGA AGATCTGCAC TATCGGCATT GCCCCCTGGG GGATTGTGGA GAACCAGGAA 
GACCTGGTGG GCAAAGATGT GGTCCGGCCA TACCAGACGA TGTCTAACCC TCTTAGCAAG 
CTAACAGTAC TCAACAGCCT ACACTCACAC TTCATTTTGG CTGATAATGG CACCACCGGA 
AAGTACGGAG CAGAAGTCAA ACTGCGCAGG CAGCTGGAGA AGCACATCTC TCTGCAAAAG 
ATTAACACAC GGATTGGACA GGGTGTTCCA GTAGTGGCTC TGATAGTGGA GGGGGGTCCG 
AATGTCATCT CTATCGTCCT GGAGTACCTA AGAGACACCC CTCCGGTGCC TGTTGTGGTG 
TGTGACGGCA GCGGTCGAGC CTCTGACATC CTGGCCTTTG GGCACAAGTA CTCAGAGGAG 
GGCGGAATCA TAAACGAATC TTTGAGAGAC CAGTTATTAG TCACCATTCA GAAGACGTTC 
ACATACAGCC GGACGCAGGC CCAGCATCTC TTCATCATTC TGATGGAGTG CATGAAGAAG 
AAAGAACTGA TCACAGTGTT TCGGATGGGA TCAGAGGGAC ACCAGGACAT CGATCTAGCC 
ATTCTAACAG CTCTTCTGAA AGGAGCGAAT GCATCAGCTC CAGATCAGCT AAGTCTGGCT 
TTGGCCTGGA ACAGAGTTGA CATCGCCCGG AGCCAGATCT TCATTTACGG ACAGCAGTGG 
CCTGTTGGCT CTCTGGAGCA GTCTATGCTG GACGCGCTGG TGCTGGACCG AGTGGACTTT 
GTAAAGCTGT TGATTGAGAA CGGAGTGAGC ATGCACCGTT TCCTGACACT CTCCAGACTT 
GAAGAGCTTT ATAATACGAG GCACGGACCA TCCAACACCT TGTACCACCT TGTCAGAGAT 
GTGAAAAAGG GTAACCTGCC CCCCGACTAC CGCATTAGCC TGATTGACAT CGGATTGGTT 
ATTGAATACC TCATGGGTGG AGCCTATCGC TGCAATTACA CCAGGAAGAG ATTTCGGACC 
CTCTATCACA ACCTCTTTGG ACCCAAAAGA CCAAAAGCCC TGAAGCTGCT TGGAATGGAG 
GATGACATGC CCATTAGACG AGGGCGTCAG AAGACTACGC GGAAGCGAGA GGAGGAAGTA 
GATATTGACC TGGATGACCC AGAAATCAAT CATTTCCCTT TCCCATTCCA TGAGCTGATG 
GTTTGGGCTG TGCTGATGAA ACGGCAGAAG ATGGCACTGT TCTTCTGGCA GCATGGAGAG 
GAGGCCATGG CTAAAGCGCT GGTGGCCTGC AAGCTCTGTA AGGCAATGGC TCACGAAGCT 
TCTGAGAACG ACATGGTCGA TGACATTTCA CAGGAGCTCA ATCAAAACTC AAGGGAGTTT 
GGTCAGCTGG CTGTTGAGCT GCTCGATCAG TCCTATAAGC AGGATGAACA GATGGCTATG 
AAGCTGTTGA CCTACGAGCT GAAGAACTGG AGTAATGCCA CCTGTCTGCA GCTGGCAGTG 
GCTGCCAAGC ACCGAGACTT CATCGCCCAC ACCTGTAGCC AGATGCTGCT CACAGACATG 
TGGATGGGAC GTCTGCGCAT GCGCAAGAAC TCTGGCCTGA AGGTTATCTT GGGCCTGCTT 
CTCCCCCCTT CCATATTGAG TCTGGAGTTT AAAAATAAAG ACGAGATGTC ATACATGCCT 
CAGGATCAGG ACACATATCT GCAGGAGAAG GATGTGGATG AGCCCGAGAA ACAAGCTAAG 
GAGAAAGAGG AGGAGGACAT GGAGTTCACA GCTATGTTGG GAAACGTCAG TTCAGAAGCA 
TCGCGCAAGA AACAGGTGGA GGAGGTTCAG AACCGGCATC GGCTCATTCC ATTGGGTCGC 
AAAATATATG AGTTTTACAA CGCCCCTATC GTCAAGTTCT GGTTTCACAC GCTGGCGTAT 
GTAGGATACT TGATGCTCTT CAACTATATT GTGCTGGTGA AGATGGACCT GTGGCCTTCT 
CCTCAGGAGT GGATTGTTAT TGCCTACATA TTCACCAATG GCATCGAGAA GATGAGAGAG 
ATCCTGATGT CCGAACCTGG GAAGCTGCTT CAGAAGGTGA AAGTGTGGTT ACAGGAGTAC 
TGGAACATCA CTGACCTCAT GGCCATCCTC ATCTTCTCCA TCGGGATGGT GCTTCGTCTG 
CAGGACCCGC CGCTGATGAG CTACGGCAGG GTCATTTACT GCGTCAATAT CATTTATTGG 
TACATACGAC TCCTCGACAT CTTCGGAGTG AACAAATACC TGGGTCCATA CGTCATGATG 
ATCGGCAAGA TGATGATTGA CATGATGTAT TTTGTGATCA TCATGTTGGT GGTGCTGATG 
AGTTTCGGTG TGGCGCGTCA GGCCATCCTT AATCCCAATG AAGACCCATC CTGGATGCTG 
GCACGCAATA TCTTCTTCAT GCCATACTGG ATGATCTACG GAGAGGTGTT TGCTGACCAA 
ATCGACCCTC CTTGTGGTCA GAACATCACC ACAGAAGAAG GAGTTGTCAT GCCTCTTCCA 
CCCTGCAAAA CTGGAGCTTG GATTGTCCCT GCCATAATGG CCTGCTACCT GCTGGTGGCC 
AACATCCTGC TAGTTAACTT GCTCATTGCT GTCTTTAATA ACACTTTCTT TGAAGTCAAG 
TCCATTTCCA ACCAGGTGTG GAAGTTTCAG AGGTATCAGC TGATCATGAC CTTCCATGAA 
CGCCCGGTCC TTCCTCCTCC ACTCATCATC TTCAGCCACA TAACTATGGT TCTCAAACAC 
CTGTGCTGTC GCTGGAGGAA GCACGATGAG GATGAACGGG ACTATGGGCT GAAGCTTTTC 
ATCACTGAAG ATGAACTGAA GAAAGTGCAT GACTTTGAAG AGCAGTGCAT GGAGGAGTAT 
TTCAGAGAGA AAGATGACCG CTTCAATTCC TCCAATGACG AAAGGATACG TGTGACATCT 
GAAAGGGTGG AGAACATGGC GATGCGATTA GAGGAGGTGA ATGAGAGGGA ACATTTTATG 
AAAGCGTCCT TGCAGACTGT AGACATCCGG CTCGCCCAGA TGGAGGAGAT GATCGGCCGC 
ATTGCTGTGG CCCTAGAGAG AGTTGCTGGA ATGGACAGAG GCGAAGTTAA CAAAGCCCGG 
TCCAGGACAT CATCAGACTG CACTGATACC AATTACATCC TGCGCCAAAG CAGTTTCAAC 
AGCCAAGAAG GCAATTCCTA CAGGCTACAG GAGTCTCTGG AGCAGGGGGG CGACGAGTCC 
ATTTCTCCTA CTTCCCCAAC CGCCCTGGCT CCCCGTGTCC GCAGTCACTC CTTCTACGTG 
AGCCACTCTT CCAAAGACAG GAGTGGGGCG GACCGTGGAG AGGGCTTCTT TAAAGACAAG 
TTGTTCAGCC TCCACCGGGC CAACAGCTCA CAGTCTGTGT CCTCAGGAGC AGGTCCCAAA 
GAGTCCAAGC CAACCCCTTT GAATACTTTA TCCGTGCAGC AGCAGCTCCG CCCTTCTTCC 
TGCATAGACA TCTATGTTTC TGCCTCAGAG GACGTCCCGC CCACTGAGAG CTTCTTGGAT 
TCGGTTCGGA CAGTTCCAAC TCTGGCTCGA GACTCATCTC TTCATTCGGA GATCATGGAG 
GCGGTGCTCT CAGGGGGACG GGACTGTAGT GGAAGGGCAG GCGGCAGCGA AAGACAGTCG 
GATGGTACAG TCCTGTTTGA GGACAGCGCC GCCGCTGACC TATCGCTCTG CTCTGCCCAC 
CTGCTGCCGG ATTCCTTGCC CCCCTGGGAC CTGGACCCCT CACCGCCACC ATCAGCTGGT 
GTACTAGAGC GGTCCAAAAG TAGTCGCTTC CTGTCTACAG CAGGGCCTCT GTTTCTGGAC 
GAGCCTCATT TGGTTAAGTC ACACAGTCTG ATGTTCACAT CACGTGGGTA CTATGGTGGC 
ATGGGTGTGC AGGTGAAGGC GGCCGAATAC ACCAGCATCA CAGATTGCAT CGACACCCGT 
TGCGTCTCAA CCCCTTACCC TGTGCCAGAG CGGTCAGACT CACCTGGAGG ATCTTTCACC 
TTCGATAAGC CTCAAGACCT TGGCGTCTCG CACCCTGAGA GAGACGCTGA GCTCAGCCAC 
GCTGAGTCTG ACTTAGAGGA GCCAGCAGAG GGTTCGGGAG ACACATGTAA GGTGGGTCAG 
AGTAGCAGTA GTGGGGTAAT AGGTGCAGAC CTGGGCCTGG GCCTCGCACT AGGCCCGTTT 
TGCTCACCCA TCTCTAGACT AGAGCGAGCA AACAGCTGCT CCTCTTCAGA GGAGTCGCAC 
TCCAACATCT ACTCTCGAAA GAGCTTCTCA ATAAGCGAGA GAATGGACAA GGGCCGGGGG 
AGCTCCAGGA ACCCTTTCCA GAAGGCCAGA ACCGGGGCGA GGCTGGAGGG TAAGACAGAC 
TCACTGTCTA TGAGGAAAAT GGCCAAACCG TCAGCTTTCA GAAGTTTTGA CAGCAGACAC 
AACTACACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm3 ( XM_005171928.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171985.1 Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa10543
Clone ID Related Accession (Same CDS sequence)	XM_005171928.4 , XM_005171928.3
Accession Version	XM_005171928.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5172bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transient receptor potential cation channel subfamily M member 3 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171928.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAAAA GAAAATGGGA GGGGAGGAGA GAAGGCTTCA AGGTGAGCTC CACGAGGGAG 
GAGGCACAGG GGCCTGGACG CCCCTCCACC AGAGGGCGCT TCTCCGAATC TTGGAAGAGA 
CTGAGCTCCA GACAGGGGTC TACCAAGAGG TCAGGACTGG CCTCCCAGCA AGCTGCGGCA 
CAGAAATCAT GGATAGAAAG AGCGTTCAGC AAGAGAGAAT GTGTGCACAT TATTGTAAGC 
ACCAAAGACC ACCATAGGTG CTGTTGTGGC CGTCTAATTG GTCAACATGT TGGCCTGCCC 
CCCAGCATCT CATCCAATCA GAATGAGAAG TCAGAACGCG TGCCAAAGAA TGACAGCTTG 
TCAGAGAAGT GGTCCATCAG CAAGCACACC CAGCTCAGTC CCACCGACGC TTTTGGGACC 
ATCGAGTTCC AGGGAGGAGG ACACTCTAAT AAAGCAATGT ATGTACGAGT GTCTTACGAC 
ACAAAACCAG ATTTACTGCT GCATCTGATG ACAAAGGAGT GGCAACTGGA GCTTCCCAAG 
CTGCTCATCT CTGTTCACGG AGGACTTCAA AACTTTGAGT TGCAGCCCAA ACTAAAGCAA 
GTCTTCGGCA AAGGTCTTAT AAAGGCTGCA ATGACTACCG GAGCCTGGAT CTTCACTGGA 
GGAGTTAATA CTGGAGTCAT CAGACATGTT GGTGATGCTC TGAAAGATCA TGCTTCCAAA 
TCAAGAGGGA AGATCTGCAC TATCGGCATT GCCCCCTGGG GGATTGTGGA GAACCAGGAA 
GACCTGGTGG GCAAAGATGT GGTCCGGCCA TACCAGACGA TGTCTAACCC TCTTAGCAAG 
CTAACAGTAC TCAACAGCCT ACACTCACAC TTCATTTTGG CTGATAATGG CACCACCGGA 
AAGTACGGAG CAGAAGTCAA ACTGCGCAGG CAGCTGGAGA AGCACATCTC TCTGCAAAAG 
ATTAACACAC GGATTGGACA GGGTGTTCCA GTAGTGGCTC TGATAGTGGA GGGGGGTCCG 
AATGTCATCT CTATCGTCCT GGAGTACCTA AGAGACACCC CTCCGGTGCC TGTTGTGGTG 
TGTGACGGCA GCGGTCGAGC CTCTGACATC CTGGCCTTTG GGCACAAGTA CTCAGAGGAG 
GGCGGAATCA TAAACGAATC TTTGAGAGAC CAGTTATTAG TCACCATTCA GAAGACGTTC 
ACATACAGCC GGACGCAGGC CCAGCATCTC TTCATCATTC TGATGGAGTG CATGAAGAAG 
AAAGAACTGA TCACAGTGTT TCGGATGGGA TCAGAGGGAC ACCAGGACAT CGATCTAGCC 
ATTCTAACAG CTCTTCTGAA AGGAGCGAAT GCATCAGCTC CAGATCAGCT AAGTCTGGCT 
TTGGCCTGGA ACAGAGTTGA CATCGCCCGG AGCCAGATCT TCATTTACGG ACAGCAGTGG 
CCTGTTGGCT CTCTGGAGCA GTCTATGCTG GACGCGCTGG TGCTGGACCG AGTGGACTTT 
GTAAAGCTGT TGATTGAGAA CGGAGTGAGC ATGCACCGTT TCCTGACACT CTCCAGACTT 
GAAGAGCTTT ATAATACGAG GCACGGACCA TCCAACACCT TGTACCACCT TGTCAGAGAT 
GTGAAAAAGG GTAACCTGCC CCCCGACTAC CGCATTAGCC TGATTGACAT CGGATTGGTT 
ATTGAATACC TCATGGGTGG AGCCTATCGC TGCAATTACA CCAGGAAGAG ATTTCGGACC 
CTCTATCACA ACCTCTTTGG ACCCAAAAGA CCAAAAGCCC TGAAGCTGCT TGGAATGGAG 
GATGACATGC CCATTAGACG AGGGCGTCAG AAGACTACGC GGAAGCGAGA GGAGGAAGTA 
GATATTGACC TGGATGACCC AGAAATCAAT CATTTCCCTT TCCCATTCCA TGAGCTGATG 
GTTTGGGCTG TGCTGATGAA ACGGCAGAAG ATGGCACTGT TCTTCTGGCA GCATGGAGAG 
GAGGCCATGG CTAAAGCGCT GGTGGCCTGC AAGCTCTGTA AGGCAATGGC TCACGAAGCT 
TCTGAGAACG ACATGGTCGA TGACATTTCA CAGGAGCTCA ATCAAAACTC AAGGGAGTTT 
GGTCAGCTGG CTGTTGAGCT GCTCGATCAG TCCTATAAGC AGGATGAACA GATGGCTATG 
AAGCTGTTGA CCTACGAGCT GAAGAACTGG AGTAATGCCA CCTGTCTGCA GCTGGCAGTG 
GCTGCCAAGC ACCGAGACTT CATCGCCCAC ACCTGTAGCC AGATGCTGCT CACAGACATG 
TGGATGGGAC GTCTGCGCAT GCGCAAGAAC TCTGGCCTGA AGGTTATCTT GGGCCTGCTT 
CTCCCCCCTT CCATATTGAG TCTGGAGTTT AAAAATAAAG ACGAGATGTC ATACATGCCT 
CAGGATCAGG ACACATATCT GCAGGAGAAG GATGTGGATG AGCCCGAGAA ACAAGCTAAG 
GAGAAAGAGG AGGAGGACAT GGAGTTCACA GCTATGTTGG GAAACGTCAG TTCAGAAGCA 
TCGCGCAAGA AACAGGTGGA GGAGGTTCAG AACCGGCATC GGCTCATTCC ATTGGGTCGC 
AAAATATATG AGTTTTACAA CGCCCCTATC GTCAAGTTCT GGTTTCACAC GCTGGCGTAT 
GTAGGATACT TGATGCTCTT CAACTATATT GTGCTGGTGA AGATGGACCT GTGGCCTTCT 
CCTCAGGAGT GGATTGTTAT TGCCTACATA TTCACCAATG GCATCGAGAA GATGAGAGAG 
ATCCTGATGT CCGAACCTGG GAAGCTGCTT CAGAAGGTGA AAGTGTGGTT ACAGGAGTAC 
TGGAACATCA CTGACCTCAT GGCCATCCTC ATCTTCTCCA TCGGGATGGT GCTTCGTCTG 
CAGGACCCGC CGCTGATGAG CTACGGCAGG GTCATTTACT GCGTCAATAT CATTTATTGG 
TACATACGAC TCCTCGACAT CTTCGGAGTG AACAAATACC TGGGTCCATA CGTCATGATG 
ATCGGCAAGA TGATGATTGA CATGATGTAT TTTGTGATCA TCATGTTGGT GGTGCTGATG 
AGTTTCGGTG TGGCGCGTCA GGCCATCCTT AATCCCAATG AAGACCCATC CTGGATGCTG 
GCACGCAATA TCTTCTTCAT GCCATACTGG ATGATCTACG GAGAGGTGTT TGCTGACCAA 
ATCGACCCTC CTTGTGGTCA GAACATCACC ACAGAAGAAG GAGTTGTCAT GCCTCTTCCA 
CCCTGCAAAA CTGGAGCTTG GATTGTCCCT GCCATAATGG CCTGCTACCT GCTGGTGGCC 
AACATCCTGC TAGTTAACTT GCTCATTGCT GTCTTTAATA ACACTTTCTT TGAAGTCAAG 
TCCATTTCCA ACCAGGTGTG GAAGTTTCAG AGGTATCAGC TGATCATGAC CTTCCATGAA 
CGCCCGGTCC TTCCTCCTCC ACTCATCATC TTCAGCCACA TAACTATGGT TCTCAAACAC 
CTGTGCTGTC GCTGGAGGAA GCACGATGAG GATGAACGGG ACTATGGGCT GAAGCTTTTC 
ATCACTGAAG ATGAACTGAA GAAAGTGCAT GACTTTGAAG AGCAGTGCAT GGAGGAGTAT 
TTCAGAGAGA AAGATGACCG CTTCAATTCC TCCAATGACG AAAGGATACG TGTGACATCT 
GAAAGGGTGG AGAACATGGC GATGCGATTA GAGGAGGTGA ATGAGAGGGA ACATTTTATG 
AAAGCGTCCT TGCAGACTGT AGACATCCGG CTCGCCCAGA TGGAGGAGAT GATCGGCCGC 
ATTGCTGTGG CCCTAGAGAG AGTTGCTGGA ATGGACAGAG GCGAAGTTAA CAAAGCCCGG 
TCCAGGACAT CATCAGACTG CACTGATACC AATTACATCC TGCGCCAAAG CAGTTTCAAC 
AGCCAAGAAG GCAATTCCTA CAGGCTACAG GAGTCTCTGG AGCAGGGGGG CGACGAGTCC 
ATTTCTCCTA CTTCCCCAAC CGCCCTGGCT CCCCGTGTCC GCAGTCACTC CTTCTACGTG 
AGCCACTCTT CCAAAGACAG GAGTGGGGCG GACCGTGGAG AGGGCTTCTT TAAAGACAAG 
TTGTTCAGCC TCCACCGGGC CAACAGCTCA CAGTCTGTGT CCTCAGGAGC AGGTCCCAAA 
GAGTCCAAGC CAACCCCTTT GAATACTTTA TCCGTGCAGC AGCAGCTCCG CCCTTCTTCC 
TGCATAGACA TCTATGTTTC TGCCTCAGAG GACGTCCCGC CCACTGAGAG CTTCTTGGAT 
TCGGTTCGGA CAGTTCCAAC TCTGGCTCGA GACTCATCTC TTCATTCGGA GATCATGGAG 
GCGGTGCTCT CAGGGGGACG GGACTGTAGT GGAAGGGCAG GCGGCAGCGA AAGACAGTCG 
GATGGTACAG TCCTGTTTGA GGACAGCGCC GCCGCTGACC TATCGCTCTG CTCTGCCCAC 
CTGCTGCCGG ATTCCTTGCC CCCCTGGGAC CTGGACCCCT CACCGCCACC ATCAGCTGGT 
GTACTAGAGC GGTCCAAAAG TAGTCGCTTC CTGTCTACAG CAGGGCCTCT GTTTCTGGAC 
GAGCCTCATT TGGTTAAGTC ACACAGTCTG ATGTTCACAT CACGTGGGTA CTATGGTGGC 
ATGGGTGTGC AGGTGAAGGC GGCCGAATAC ACCAGCATCA CAGATTGCAT CGACACCCGT 
TGCGTCTCAA CCCCTTACCC TGTGCCAGAG CGGTCAGACT CACCTGGAGG ATCTTTCACC 
TTCGATAAGC CTCAAGACCT TGGCGTCTCG CACCCTGAGA GAGACGCTGA GCTCAGCCAC 
GCTGAGTCTG ACTTAGAGGA GCCAGCAGAG GGTTCGGGAG ACACATGTAA GGTGGGTCAG 
AGTAGCAGTA GTGGGGTAAT AGGTGCAGAC CTGGGCCTGG GCCTCGCACT AGGCCCGTTT 
TGCTCACCCA TCTCTAGACT AGAGCGAGCA AACAGCTGCT CCTCTTCAGA GGAGTCGCAC 
TCCAACATCT ACTCTCGAAA GAGCTTCTCA ATAAGCGAGA GAATGGACAA GGGCCGGGGG 
AGCTCCAGGA ACCCTTTCCA GAAGGCCAGA ACCGGGGCGA GGCTGGAGGG TAAGACAGAC 
TCACTGTCTA TGAGGAAAAT GGCCAAACCG TCAGCTTTCA GAAGTTTTGA CAGCAGACAC 
AACTACACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171985.1
CDS	338..5509
Translation	MTKRKWEGRREGFKVSSTREEAQGPGRPSTRGRFSESWKRLSSRQGSTKRSGLASQQAAAQKSWIERAFSKRECVHIIVSTKDHHRCCCGRLIGQHVGLPPSISSNQNEKSERVPKNDSLSEKWSISKHTQLSPTDAFGTIEFQGGGHSNKAMYVRVSYDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLVGKDVVRPYQTMSNPLSKLTVLNSLHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGIINESLRDQLLVTIQKTFTYSRTQAQHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQSMLDALVLDRVDFVKLLIENGVSMHRFLTLSRLEELYNTRHGPSNTLYHLVRDVKKGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDMPIRRGRQKTTRKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNQNSREFGQLAVELLDQSYKQDEQMAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGLLLPPSILSLEFKNKDEMSYMPQDQDTYLQEKDVDEPEKQAKEKEEEDMEFTAMLGNVSSEASRKKQVEEVQNRHRLIPLGRKIYEFYNAPIVKFWFHTLAYVGYLMLFNYIVLVKMDLWPSPQEWIVIAYIFTNGIEKMREILMSEPGKLLQKVKVWLQEYWNITDLMAILIFSIGMVLRLQDPPLMSYGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDPPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHITMVLKHLCCRWRKHDEDERDYGLKLFITEDELKKVHDFEEQCMEEYFREKDDRFNSSNDERIRVTSERVENMAMRLEEVNEREHFMKASLQTVDIRLAQMEEMIGRIAVALERVAGMDRGEVNKARSRTSSDCTDTNYILRQSSFNSQEGNSYRLQESLEQGGDESISPTSPTALAPRVRSHSFYVSHSSKDRSGADRGEGFFKDKLFSLHRANSSQSVSSGAGPKESKPTPLNTLSVQQQLRPSSCIDIYVSASEDVPPTESFLDSVRTVPTLARDSSLHSEIMEAVLSGGRDCSGRAGGSERQSDGTVLFEDSAAADLSLCSAHLLPDSLPPWDLDPSPPPSAGVLERSKSSRFLSTAGPLFLDEPHLVKSHSLMFTSRGYYGGMGVQVKAAEYTSITDCIDTRCVSTPYPVPERSDSPGGSFTFDKPQDLGVSHPERDAELSHAESDLEEPAEGSGDTCKVGQSSSSGVIGADLGLGLALGPFCSPISRLERANSCSSSEESHSNIYSRKSFSISERMDKGRGSSRNPFQKARTGARLEGKTDSLSMRKMAKPSAFRSFDSRHNYT

Target ORF information:

RefSeq Version	XM_005171928.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171928.3

ORF Insert Sequence:

ATGACAAAAA GAAAATGGGA GGGGAGGAGA GAAGGCTTCA AGGTGAGCTC CACGAGGGAG 
GAGGCACAGG GGCCTGGACG CCCCTCCACC AGAGGGCGCT TCTCCGAATC TTGGAAGAGA 
CTGAGCTCCA GACAGGGGTC TACCAAGAGG TCAGGACTGG CCTCCCAGCA AGCTGCGGCA 
CAGAAATCAT GGATAGAAAG AGCGTTCAGC AAGAGAGAAT GTGTGCACAT TATTGTAAGC 
ACCAAAGACC ACCATAGGTG CTGTTGTGGC CGTCTAATTG GTCAACATGT TGGCCTGCCC 
CCCAGCATCT CATCCAATCA GAATGAGAAG TCAGAACGCG TGCCAAAGAA TGACAGCTTG 
TCAGAGAAGT GGTCCATCAG CAAGCACACC CAGCTCAGTC CCACCGACGC TTTTGGGACC 
ATCGAGTTCC AGGGAGGAGG ACACTCTAAT AAAGCAATGT ATGTACGAGT GTCTTACGAC 
ACAAAACCAG ATTTACTGCT GCATCTGATG ACAAAGGAGT GGCAACTGGA GCTTCCCAAG 
CTGCTCATCT CTGTTCACGG AGGACTTCAA AACTTTGAGT TGCAGCCCAA ACTAAAGCAA 
GTCTTCGGCA AAGGTCTTAT AAAGGCTGCA ATGACTACCG GAGCCTGGAT CTTCACTGGA 
GGAGTTAATA CTGGAGTCAT CAGACATGTT GGTGATGCTC TGAAAGATCA TGCTTCCAAA 
TCAAGAGGGA AGATCTGCAC TATCGGCATT GCCCCCTGGG GGATTGTGGA GAACCAGGAA 
GACCTGGTGG GCAAAGATGT GGTCCGGCCA TACCAGACGA TGTCTAACCC TCTTAGCAAG 
CTAACAGTAC TCAACAGCCT ACACTCACAC TTCATTTTGG CTGATAATGG CACCACCGGA 
AAGTACGGAG CAGAAGTCAA ACTGCGCAGG CAGCTGGAGA AGCACATCTC TCTGCAAAAG 
ATTAACACAC GGATTGGACA GGGTGTTCCA GTAGTGGCTC TGATAGTGGA GGGGGGTCCG 
AATGTCATCT CTATCGTCCT GGAGTACCTA AGAGACACCC CTCCGGTGCC TGTTGTGGTG 
TGTGACGGCA GCGGTCGAGC CTCTGACATC CTGGCCTTTG GGCACAAGTA CTCAGAGGAG 
GGCGGAATCA TAAACGAATC TTTGAGAGAC CAGTTATTAG TCACCATTCA GAAGACGTTC 
ACATACAGCC GGACGCAGGC CCAGCATCTC TTCATCATTC TGATGGAGTG CATGAAGAAG 
AAAGAACTGA TCACAGTGTT TCGGATGGGA TCAGAGGGAC ACCAGGACAT CGATCTAGCC 
ATTCTAACAG CTCTTCTGAA AGGAGCGAAT GCATCAGCTC CAGATCAGCT AAGTCTGGCT 
TTGGCCTGGA ACAGAGTTGA CATCGCCCGG AGCCAGATCT TCATTTACGG ACAGCAGTGG 
CCTGTTGGCT CTCTGGAGCA GTCTATGCTG GACGCGCTGG TGCTGGACCG AGTGGACTTT 
GTAAAGCTGT TGATTGAGAA CGGAGTGAGC ATGCACCGTT TCCTGACACT CTCCAGACTT 
GAAGAGCTTT ATAATACGAG GCACGGACCA TCCAACACCT TGTACCACCT TGTCAGAGAT 
GTGAAAAAGG GTAACCTGCC CCCCGACTAC CGCATTAGCC TGATTGACAT CGGATTGGTT 
ATTGAATACC TCATGGGTGG AGCCTATCGC TGCAATTACA CCAGGAAGAG ATTTCGGACC 
CTCTATCACA ACCTCTTTGG ACCCAAAAGA CCAAAAGCCC TGAAGCTGCT TGGAATGGAG 
GATGACATGC CCATTAGACG AGGGCGTCAG AAGACTACGC GGAAGCGAGA GGAGGAAGTA 
GATATTGACC TGGATGACCC AGAAATCAAT CATTTCCCTT TCCCATTCCA TGAGCTGATG 
GTTTGGGCTG TGCTGATGAA ACGGCAGAAG ATGGCACTGT TCTTCTGGCA GCATGGAGAG 
GAGGCCATGG CTAAAGCGCT GGTGGCCTGC AAGCTCTGTA AGGCAATGGC TCACGAAGCT 
TCTGAGAACG ACATGGTCGA TGACATTTCA CAGGAGCTCA ATCAAAACTC AAGGGAGTTT 
GGTCAGCTGG CTGTTGAGCT GCTCGATCAG TCCTATAAGC AGGATGAACA GATGGCTATG 
AAGCTGTTGA CCTACGAGCT GAAGAACTGG AGTAATGCCA CCTGTCTGCA GCTGGCAGTG 
GCTGCCAAGC ACCGAGACTT CATCGCCCAC ACCTGTAGCC AGATGCTGCT CACAGACATG 
TGGATGGGAC GTCTGCGCAT GCGCAAGAAC TCTGGCCTGA AGGTTATCTT GGGCCTGCTT 
CTCCCCCCTT CCATATTGAG TCTGGAGTTT AAAAATAAAG ACGAGATGTC ATACATGCCT 
CAGGATCAGG ACACATATCT GCAGGAGAAG GATGTGGATG AGCCCGAGAA ACAAGCTAAG 
GAGAAAGAGG AGGAGGACAT GGAGTTCACA GCTATGTTGG GAAACGTCAG TTCAGAAGCA 
TCGCGCAAGA AACAGGTGGA GGAGGTTCAG AACCGGCATC GGCTCATTCC ATTGGGTCGC 
AAAATATATG AGTTTTACAA CGCCCCTATC GTCAAGTTCT GGTTTCACAC GCTGGCGTAT 
GTAGGATACT TGATGCTCTT CAACTATATT GTGCTGGTGA AGATGGACCT GTGGCCTTCT 
CCTCAGGAGT GGATTGTTAT TGCCTACATA TTCACCAATG GCATCGAGAA GATGAGAGAG 
ATCCTGATGT CCGAACCTGG GAAGCTGCTT CAGAAGGTGA AAGTGTGGTT ACAGGAGTAC 
TGGAACATCA CTGACCTCAT GGCCATCCTC ATCTTCTCCA TCGGGATGGT GCTTCGTCTG 
CAGGACCCGC CGCTGATGAG CTACGGCAGG GTCATTTACT GCGTCAATAT CATTTATTGG 
TACATACGAC TCCTCGACAT CTTCGGAGTG AACAAATACC TGGGTCCATA CGTCATGATG 
ATCGGCAAGA TGATGATTGA CATGATGTAT TTTGTGATCA TCATGTTGGT GGTGCTGATG 
AGTTTCGGTG TGGCGCGTCA GGCCATCCTT AATCCCAATG AAGACCCATC CTGGATGCTG 
GCACGCAATA TCTTCTTCAT GCCATACTGG ATGATCTACG GAGAGGTGTT TGCTGACCAA 
ATCGACCCTC CTTGTGGTCA GAACATCACC ACAGAAGAAG GAGTTGTCAT GCCTCTTCCA 
CCCTGCAAAA CTGGAGCTTG GATTGTCCCT GCCATAATGG CCTGCTACCT GCTGGTGGCC 
AACATCCTGC TAGTTAACTT GCTCATTGCT GTCTTTAATA ACACTTTCTT TGAAGTCAAG 
TCCATTTCCA ACCAGGTGTG GAAGTTTCAG AGGTATCAGC TGATCATGAC CTTCCATGAA 
CGCCCGGTCC TTCCTCCTCC ACTCATCATC TTCAGCCACA TAACTATGGT TCTCAAACAC 
CTGTGCTGTC GCTGGAGGAA GCACGATGAG GATGAACGGG ACTATGGGCT GAAGCTTTTC 
ATCACTGAAG ATGAACTGAA GAAAGTGCAT GACTTTGAAG AGCAGTGCAT GGAGGAGTAT 
TTCAGAGAGA AAGATGACCG CTTCAATTCC TCCAATGACG AAAGGATACG TGTGACATCT 
GAAAGGGTGG AGAACATGGC GATGCGATTA GAGGAGGTGA ATGAGAGGGA ACATTTTATG 
AAAGCGTCCT TGCAGACTGT AGACATCCGG CTCGCCCAGA TGGAGGAGAT GATCGGCCGC 
ATTGCTGTGG CCCTAGAGAG AGTTGCTGGA ATGGACAGAG GCGAAGTTAA CAAAGCCCGG 
TCCAGGACAT CATCAGACTG CACTGATACC AATTACATCC TGCGCCAAAG CAGTTTCAAC 
AGCCAAGAAG GCAATTCCTA CAGGCTACAG GAGTCTCTGG AGCAGGGGGG CGACGAGTCC 
ATTTCTCCTA CTTCCCCAAC CGCCCTGGCT CCCCGTGTCC GCAGTCACTC CTTCTACGTG 
AGCCACTCTT CCAAAGACAG GAGTGGGGCG GACCGTGGAG AGGGCTTCTT TAAAGACAAG 
TTGTTCAGCC TCCACCGGGC CAACAGCTCA CAGTCTGTGT CCTCAGGAGC AGGTCCCAAA 
GAGTCCAAGC CAACCCCTTT GAATACTTTA TCCGTGCAGC AGCAGCTCCG CCCTTCTTCC 
TGCATAGACA TCTATGTTTC TGCCTCAGAG GACGTCCCGC CCACTGAGAG CTTCTTGGAT 
TCGGTTCGGA CAGTTCCAAC TCTGGCTCGA GACTCATCTC TTCATTCGGA GATCATGGAG 
GCGGTGCTCT CAGGGGGACG GGACTGTAGT GGAAGGGCAG GCGGCAGCGA AAGACAGTCG 
GATGGTACAG TCCTGTTTGA GGACAGCGCC GCCGCTGACC TATCGCTCTG CTCTGCCCAC 
CTGCTGCCGG ATTCCTTGCC CCCCTGGGAC CTGGACCCCT CACCGCCACC ATCAGCTGGT 
GTACTAGAGC GGTCCAAAAG TAGTCGCTTC CTGTCTACAG CAGGGCCTCT GTTTCTGGAC 
GAGCCTCATT TGGTTAAGTC ACACAGTCTG ATGTTCACAT CACGTGGGTA CTATGGTGGC 
ATGGGTGTGC AGGTGAAGGC GGCCGAATAC ACCAGCATCA CAGATTGCAT CGACACCCGT 
TGCGTCTCAA CCCCTTACCC TGTGCCAGAG CGGTCAGACT CACCTGGAGG ATCTTTCACC 
TTCGATAAGC CTCAAGACCT TGGCGTCTCG CACCCTGAGA GAGACGCTGA GCTCAGCCAC 
GCTGAGTCTG ACTTAGAGGA GCCAGCAGAG GGTTCGGGAG ACACATGTAA GGTGGGTCAG 
AGTAGCAGTA GTGGGGTAAT AGGTGCAGAC CTGGGCCTGG GCCTCGCACT AGGCCCGTTT 
TGCTCACCCA TCTCTAGACT AGAGCGAGCA AACAGCTGCT CCTCTTCAGA GGAGTCGCAC 
TCCAACATCT ACTCTCGAAA GAGCTTCTCA ATAAGCGAGA GAATGGACAA GGGCCGGGGG 
AGCTCCAGGA ACCCTTTCCA GAAGGCCAGA ACCGGGGCGA GGCTGGAGGG TAAGACAGAC 
TCACTGTCTA TGAGGAAAAT GGCCAAACCG TCAGCTTTCA GAAGTTTTGA CAGCAGACAC 
AACTACACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm3 ( XM_005171929.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171986.1 Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa10544
Clone ID Related Accession (Same CDS sequence)	XM_005171929.3 , XM_005171929.4
Accession Version	XM_005171929.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transient receptor potential cation channel subfamily M member 3 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171929.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAAAA GAAAATGGGA GGGGAGGAGA GAAGGCTTCA AGGTGAGCTC CACGAGGGAG 
GAGGCACAGG GGCCTGGACG CCCCTCCACC AGAGGGCGCT TCTCCGAATC TTGGAAGAGA 
CTGAGCTCCA GACAGGGGTC TACCAAGAGG TCAGGACTGG CCTCCCAGCA AGCTGCGGCA 
CAGAAATCAT GGATAGAAAG AGCGTTCAGC AAGAGAGAAT GTGTGCACAT TATTGTAAGC 
ACCAAAGACC ACCATAGGTG CTGTTGTGGC CGTCTAATTG GTCAACATGT TGGCCTGCCC 
CCCAGCATCT CATCCAATCA GAATGAGAAG TCAGAACGCG TGCCAAAGAA TGACAGCTTG 
TCAGAGAAGT GGTCCATCAG CAAGCACACC CAGCTCAGTC CCACCGACGC TTTTGGGACC 
ATCGAGTTCC AGGGAGGAGG ACACTCTAAT AAAGCAATGT ATGTACGAGT GTCTTACGAC 
ACAAAACCAG ATTTACTGCT GCATCTGATG ACAAAGGAGT GGCAACTGGA GCTTCCCAAG 
CTGCTCATCT CTGTTCACGG AGGACTTCAA AACTTTGAGT TGCAGCCCAA ACTAAAGCAA 
GTCTTCGGCA AAGGTCTTAT AAAGGCTGCA ATGACTACCG GAGCCTGGAT CTTCACTGGA 
GGAGTTAATA CTGGAGTCAT CAGACATGTT GGTGATGCTC TGAAAGATCA TGCTTCCAAA 
TCAAGAGGGA AGATCTGCAC TATCGGCATT GCCCCCTGGG GGATTGTGGA GAACCAGGAA 
GACCTGGTGG GCAAAGATGT GGTCCGGCCA TACCAGACGA TGTCTAACCC TCTTAGCAAG 
CTAACAGTAC TCAACAGCCT ACACTCACAC TTCATTTTGG CTGATAATGG CACCACCGGA 
AAGTACGGAG CAGAAGTCAA ACTGCGCAGG CAGCTGGAGA AGCACATCTC TCTGCAAAAG 
ATTAACACAC GGATTGGACA GGGTGTTCCA GTAGTGGCTC TGATAGTGGA GGGGGGTCCG 
AATGTCATCT CTATCGTCCT GGAGTACCTA AGAGACACCC CTCCGGTGCC TGTTGTGGTG 
TGTGACGGCA GCGGTCGAGC CTCTGACATC CTGGCCTTTG GGCACAAGTA CTCAGAGGAG 
GGCGGAATCA TAAACGAATC TTTGAGAGAC CAGTTATTAG TCACCATTCA GAAGACGTTC 
ACATACAGCC GGACGCAGGC CCAGCATCTC TTCATCATTC TGATGGAGTG CATGAAGAAG 
AAAGAACTGA TCACAGTGTT TCGGATGGGA TCAGAGGGAC ACCAGGACAT CGATCTAGCC 
ATTCTAACAG CTCTTCTGAA AGGAGCGAAT GCATCAGCTC CAGATCAGCT AAGTCTGGCT 
TTGGCCTGGA ACAGAGTTGA CATCGCCCGG AGCCAGATCT TCATTTACGG ACAGCAGTGG 
CCTGTTGGCT CTCTGGAGCA GTCTATGCTG GACGCGCTGG TGCTGGACCG AGTGGACTTT 
GTAAAGCTGT TGATTGAGAA CGGAGTGAGC ATGCACCGTT TCCTGACACT CTCCAGACTT 
GAAGAGCTTT ATAATACGAG GCACGGACCA TCCAACACCT TGTACCACCT TGTCAGAGAT 
GTGAAAAAGC AAGAATATCC AGGGTTCAGT TGGATCTACC TCAAGGGTAA CCTGCCCCCC 
GACTACCGCA TTAGCCTGAT TGACATCGGA TTGGTTATTG AATACCTCAT GGGTGGAGCC 
TATCGCTGCA ATTACACCAG GAAGAGATTT CGGACCCTCT ATCACAACCT CTTTGGACCC 
AAAAGACCAA AAGCCCTGAA GCTGCTTGGA ATGGAGGATG ACATGCCCAT TAGACGAGGG 
CGTCAGAAGA CTACGCGGAA GCGAGAGGAG GAAGTAGATA TTGACCTGGA TGACCCAGAA 
ATCAATCATT TCCCTTTCCC ATTCCATGAG CTGATGGTTT GGGCTGTGCT GATGAAACGG 
CAGAAGATGG CACTGTTCTT CTGGCAGCAT GGAGAGGAGG CCATGGCTAA AGCGCTGGTG 
GCCTGCAAGC TCTGTAAGGC AATGGCTCAC GAAGCTTCTG AGAACGACAT GGTCGATGAC 
ATTTCACAGG AGCTCAATCA AAACTCAAGG GAGTTTGGTC AGCTGGCTGT TGAGCTGCTC 
GATCAGTCCT ATAAGCAGGA TGAACAGATG GCTATGAAGC TGTTGACCTA CGAGCTGAAG 
AACTGGAGTA ATGCCACCTG TCTGCAGCTG GCAGTGGCTG CCAAGCACCG AGACTTCATC 
GCCCACACCT GTAGCCAGAT GCTGCTCACA GACATGTGGA TGGGACGTCT GCGCATGCGC 
AAGAACTCTG GCCTGAAGGT TATCTTGGGC CTGCTTCTCC CCCCTTCCAT ATTGAGTCTG 
GAGTTTAAAA ATAAAGACGA GATGTCATAC ATGCCTCAGG ATCAGGACAC ATATCTGCAG 
GAGAAGGATG TGGATGAGCC CGAGAAACAA GCTAAGGAGA AAGAGGAGGA GGACATGGAG 
TTCACAGCTA TGTTGGGAAA CGTCAGTTCA GAAGCATCGC GCAAGAAACA GGTGGAGGAG 
GTTCAGAACC GGCATCGGCT CATTCCATTG GGTCGCAAAA TATATGAGTT TTACAACGCC 
CCTATCGTCA AGTTCTGGTT TCACACGCTG GCGTATGTAG GATACTTGAT GCTCTTCAAC 
TATATTGTGC TGGTGAAGAT GGACCTGTGG CCTTCTCCTC AGGAGTGGAT TGTTATTGCC 
TACATATTCA CCAATGGCAT CGAGAAGATG AGAGAGATCC TGATGTCCGA ACCTGGGAAG 
CTGCTTCAGA AGGTGAAAGT GTGGTTACAG GAGTACTGGA ACATCACTGA CCTCATGGCC 
ATCCTCATCT TCTCCATCGG GATGGTGCTT CGTCTGCAGG ACCCGCCGCT GATGAGCTAC 
GGCAGGGTCA TTTACTGCGT CAATATCATT TATTGGTACA TACGACTCCT CGACATCTTC 
GGAGTGAACA AATACCTGGG TCCATACGTC ATGATGATCG GCAAGATGAT GATTGACATG 
ATGTATTTTG TGATCATCAT GTTGGTGGTG CTGATGAGTT TCGGTGTGGC GCGTCAGGCC 
ATCCTTAATC CCAATGAAGA CCCATCCTGG ATGCTGGCAC GCAATATCTT CTTCATGCCA 
TACTGGATGA TCTACGGAGA GGTGTTTGCT GACCAAATCG ACCCTCCTTG TGGTCAGAAC 
ATCACCACAG AAGAAGGAGT TGTCATGCCT CTTCCACCCT GCAAAACTGG AGCTTGGATT 
GTCCCTGCCA TAATGGCCTG CTACCTGCTG GTGGCCAACA TCCTGCTAGT TAACTTGCTC 
ATTGCTGTCT TTAATAACAC TTTCTTTGAA GTCAAGTCCA TTTCCAACCA GGTGTGGAAG 
TTTCAGAGGT ATCAGCTGAT CATGACCTTC CATGAACGCC CGGTCCTTCC TCCTCCACTC 
ATCATCTTCA GCCACATAAC TATGGTTCTC AAACACCTGT GCTGTCGCTG GAGGAAGCAC 
GATGAGGATG AACGGGACTA TGGGCTGAAG CTTTTCATCA CTGAAGATGA ACTGAAGAAA 
GTGCATGACT TTGAAGAGCA GTGCATGGAG GAGTATTTCA GAGAGAAAGA TGACCGCTTC 
AATTCCTCCA ATGACGAAAG GATACGTGTG ACATCTGAAA GGGTGGAGAA CATGGCGATG 
CGATTAGAGG AGGTGAATGA GAGGGAACAT TTTATGAAAG CGTCCTTGCA GACTGTAGAC 
ATCCGGCTCG CCCAGATGGA GGAGATGATC GGCCGCATTG CTGTGGCCCT AGAGAGAGTT 
GCTGGAATGG ACAGAGGCGA AGTTAACAAA GCCCGGTCCA GGACATCATC AGACTGCACT 
GATACCAATT ACATCCTGCG CCAAAGCAGT TTCAACAGCC AAGAAGGCAA TTCCTACAGG 
CTACAGGAGT CTCTGGAGCA GGGGGGCGAC GAGTCCATTT CTCCTACTTC CCCAACCGCC 
CTGGCTCCCC GTGTCCGCAG TCACTCCTTC TACGTGAGCC ACTCTTCCAA AGACAGGAGT 
GGGGCGGACC GTGGAGAGGG CTTCTTTAAA GACAAGTTGT TCAGCCTCCA CCGGGCCAAC 
AGCTCACAGT CTGTGTCCTC AGGAGCAGGT CCCAAAGAGT CCAAGCCAAC CCCTTTGAAT 
ACTTTATCCG TGCAGCAGCA GCTCCGCCCT TCTTCCTGCA TAGACATCTA TGTTTCTGCC 
TCAGAGGACG TCCCGCCCAC TGAGAGCTTC TTGGATTCGG TTCGGACAGT TCCAACTCTG 
GCTCGAGACT CATCTCTTCA TTCGGAGATC ATGGAGGCGG TGCTCTCAGG GGGACGGGAC 
TGTAGTGGAA GGGCAGGCGG CAGCGAAAGA CAGTCGGATG GTACAGTCCT GTTTGAGGAC 
AGCGCCGCCG CTGACCTATC GCTCTGCTCT GCCCACCTGC TGCCGGATTC CTTGCCCCCC 
TGGGACCTGG ACCCCTCACC GCCACCATCA GCTGGTGTAC TAGAGCGGTC CAAAAGTAGT 
CGCTTCCTGT CTACAGCAGG GCCTCTGTTT CTGGACGAGC CTCATTTGGT TAAGTCACAC 
AGTCTGATGT TCACATCACG TGGGTACTAT GGTGGCATGG GTGTGCAGGT GAAGGCGGCC 
GAATACACCA GCATCACAGA TTGCATCGAC ACCCGTTGCG TCTCAACCCC TTACCCTGTG 
CCAGAGCGGT CAGACTCACC TGGAGGATCT TTCACCTTCG ATAAGCCTCA AGACCTTGGC 
GTCTCGCACC CTGAGAGAGA CGCTGAGCTC AGCCACGCTG AGTCTGACTT AGAGGAGCCA 
GCAGAGGGTT CGGGAGACAC ATGTAAGGTG GGTCAGAGTA GCAGTAGTGG GGTAATAGGT 
GCAGACCTGG GCCTGGGCCT CGCACTAGGC CCGTTTTGCT CACCCATCTC TAGACTAGAG 
CGAGCAAACA GCTGCTCCTC TTCAGAGGAG TCGCACTCCA ACATCTACTC TCGAAAGAGC 
TTCTCAATAA GCGAGAGAAT GGACAAGGGC CGGGGGAGCT CCAGGAACCC TTTCCAGAAG 
GCCAGAACCG GGGCGAGGCT GGAGGGTAAG ACAGACTCAC TGTCTATGAG GAAAATGGCC 
AAACCGTCAG CTTTCAGAAG TTTTGACAGC AGACACAACT ACACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171986.1
CDS	338..5545
Translation	MTKRKWEGRREGFKVSSTREEAQGPGRPSTRGRFSESWKRLSSRQGSTKRSGLASQQAAAQKSWIERAFSKRECVHIIVSTKDHHRCCCGRLIGQHVGLPPSISSNQNEKSERVPKNDSLSEKWSISKHTQLSPTDAFGTIEFQGGGHSNKAMYVRVSYDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLVGKDVVRPYQTMSNPLSKLTVLNSLHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGIINESLRDQLLVTIQKTFTYSRTQAQHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQSMLDALVLDRVDFVKLLIENGVSMHRFLTLSRLEELYNTRHGPSNTLYHLVRDVKKQEYPGFSWIYLKGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDMPIRRGRQKTTRKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNQNSREFGQLAVELLDQSYKQDEQMAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGLLLPPSILSLEFKNKDEMSYMPQDQDTYLQEKDVDEPEKQAKEKEEEDMEFTAMLGNVSSEASRKKQVEEVQNRHRLIPLGRKIYEFYNAPIVKFWFHTLAYVGYLMLFNYIVLVKMDLWPSPQEWIVIAYIFTNGIEKMREILMSEPGKLLQKVKVWLQEYWNITDLMAILIFSIGMVLRLQDPPLMSYGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDPPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHITMVLKHLCCRWRKHDEDERDYGLKLFITEDELKKVHDFEEQCMEEYFREKDDRFNSSNDERIRVTSERVENMAMRLEEVNEREHFMKASLQTVDIRLAQMEEMIGRIAVALERVAGMDRGEVNKARSRTSSDCTDTNYILRQSSFNSQEGNSYRLQESLEQGGDESISPTSPTALAPRVRSHSFYVSHSSKDRSGADRGEGFFKDKLFSLHRANSSQSVSSGAGPKESKPTPLNTLSVQQQLRPSSCIDIYVSASEDVPPTESFLDSVRTVPTLARDSSLHSEIMEAVLSGGRDCSGRAGGSERQSDGTVLFEDSAAADLSLCSAHLLPDSLPPWDLDPSPPPSAGVLERSKSSRFLSTAGPLFLDEPHLVKSHSLMFTSRGYYGGMGVQVKAAEYTSITDCIDTRCVSTPYPVPERSDSPGGSFTFDKPQDLGVSHPERDAELSHAESDLEEPAEGSGDTCKVGQSSSSGVIGADLGLGLALGPFCSPISRLERANSCSSSEESHSNIYSRKSFSISERMDKGRGSSRNPFQKARTGARLEGKTDSLSMRKMAKPSAFRSFDSRHNYT

Target ORF information:

RefSeq Version	XM_005171929.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171929.3

ORF Insert Sequence:

ATGACAAAAA GAAAATGGGA GGGGAGGAGA GAAGGCTTCA AGGTGAGCTC CACGAGGGAG 
GAGGCACAGG GGCCTGGACG CCCCTCCACC AGAGGGCGCT TCTCCGAATC TTGGAAGAGA 
CTGAGCTCCA GACAGGGGTC TACCAAGAGG TCAGGACTGG CCTCCCAGCA AGCTGCGGCA 
CAGAAATCAT GGATAGAAAG AGCGTTCAGC AAGAGAGAAT GTGTGCACAT TATTGTAAGC 
ACCAAAGACC ACCATAGGTG CTGTTGTGGC CGTCTAATTG GTCAACATGT TGGCCTGCCC 
CCCAGCATCT CATCCAATCA GAATGAGAAG TCAGAACGCG TGCCAAAGAA TGACAGCTTG 
TCAGAGAAGT GGTCCATCAG CAAGCACACC CAGCTCAGTC CCACCGACGC TTTTGGGACC 
ATCGAGTTCC AGGGAGGAGG ACACTCTAAT AAAGCAATGT ATGTACGAGT GTCTTACGAC 
ACAAAACCAG ATTTACTGCT GCATCTGATG ACAAAGGAGT GGCAACTGGA GCTTCCCAAG 
CTGCTCATCT CTGTTCACGG AGGACTTCAA AACTTTGAGT TGCAGCCCAA ACTAAAGCAA 
GTCTTCGGCA AAGGTCTTAT AAAGGCTGCA ATGACTACCG GAGCCTGGAT CTTCACTGGA 
GGAGTTAATA CTGGAGTCAT CAGACATGTT GGTGATGCTC TGAAAGATCA TGCTTCCAAA 
TCAAGAGGGA AGATCTGCAC TATCGGCATT GCCCCCTGGG GGATTGTGGA GAACCAGGAA 
GACCTGGTGG GCAAAGATGT GGTCCGGCCA TACCAGACGA TGTCTAACCC TCTTAGCAAG 
CTAACAGTAC TCAACAGCCT ACACTCACAC TTCATTTTGG CTGATAATGG CACCACCGGA 
AAGTACGGAG CAGAAGTCAA ACTGCGCAGG CAGCTGGAGA AGCACATCTC TCTGCAAAAG 
ATTAACACAC GGATTGGACA GGGTGTTCCA GTAGTGGCTC TGATAGTGGA GGGGGGTCCG 
AATGTCATCT CTATCGTCCT GGAGTACCTA AGAGACACCC CTCCGGTGCC TGTTGTGGTG 
TGTGACGGCA GCGGTCGAGC CTCTGACATC CTGGCCTTTG GGCACAAGTA CTCAGAGGAG 
GGCGGAATCA TAAACGAATC TTTGAGAGAC CAGTTATTAG TCACCATTCA GAAGACGTTC 
ACATACAGCC GGACGCAGGC CCAGCATCTC TTCATCATTC TGATGGAGTG CATGAAGAAG 
AAAGAACTGA TCACAGTGTT TCGGATGGGA TCAGAGGGAC ACCAGGACAT CGATCTAGCC 
ATTCTAACAG CTCTTCTGAA AGGAGCGAAT GCATCAGCTC CAGATCAGCT AAGTCTGGCT 
TTGGCCTGGA ACAGAGTTGA CATCGCCCGG AGCCAGATCT TCATTTACGG ACAGCAGTGG 
CCTGTTGGCT CTCTGGAGCA GTCTATGCTG GACGCGCTGG TGCTGGACCG AGTGGACTTT 
GTAAAGCTGT TGATTGAGAA CGGAGTGAGC ATGCACCGTT TCCTGACACT CTCCAGACTT 
GAAGAGCTTT ATAATACGAG GCACGGACCA TCCAACACCT TGTACCACCT TGTCAGAGAT 
GTGAAAAAGC AAGAATATCC AGGGTTCAGT TGGATCTACC TCAAGGGTAA CCTGCCCCCC 
GACTACCGCA TTAGCCTGAT TGACATCGGA TTGGTTATTG AATACCTCAT GGGTGGAGCC 
TATCGCTGCA ATTACACCAG GAAGAGATTT CGGACCCTCT ATCACAACCT CTTTGGACCC 
AAAAGACCAA AAGCCCTGAA GCTGCTTGGA ATGGAGGATG ACATGCCCAT TAGACGAGGG 
CGTCAGAAGA CTACGCGGAA GCGAGAGGAG GAAGTAGATA TTGACCTGGA TGACCCAGAA 
ATCAATCATT TCCCTTTCCC ATTCCATGAG CTGATGGTTT GGGCTGTGCT GATGAAACGG 
CAGAAGATGG CACTGTTCTT CTGGCAGCAT GGAGAGGAGG CCATGGCTAA AGCGCTGGTG 
GCCTGCAAGC TCTGTAAGGC AATGGCTCAC GAAGCTTCTG AGAACGACAT GGTCGATGAC 
ATTTCACAGG AGCTCAATCA AAACTCAAGG GAGTTTGGTC AGCTGGCTGT TGAGCTGCTC 
GATCAGTCCT ATAAGCAGGA TGAACAGATG GCTATGAAGC TGTTGACCTA CGAGCTGAAG 
AACTGGAGTA ATGCCACCTG TCTGCAGCTG GCAGTGGCTG CCAAGCACCG AGACTTCATC 
GCCCACACCT GTAGCCAGAT GCTGCTCACA GACATGTGGA TGGGACGTCT GCGCATGCGC 
AAGAACTCTG GCCTGAAGGT TATCTTGGGC CTGCTTCTCC CCCCTTCCAT ATTGAGTCTG 
GAGTTTAAAA ATAAAGACGA GATGTCATAC ATGCCTCAGG ATCAGGACAC ATATCTGCAG 
GAGAAGGATG TGGATGAGCC CGAGAAACAA GCTAAGGAGA AAGAGGAGGA GGACATGGAG 
TTCACAGCTA TGTTGGGAAA CGTCAGTTCA GAAGCATCGC GCAAGAAACA GGTGGAGGAG 
GTTCAGAACC GGCATCGGCT CATTCCATTG GGTCGCAAAA TATATGAGTT TTACAACGCC 
CCTATCGTCA AGTTCTGGTT TCACACGCTG GCGTATGTAG GATACTTGAT GCTCTTCAAC 
TATATTGTGC TGGTGAAGAT GGACCTGTGG CCTTCTCCTC AGGAGTGGAT TGTTATTGCC 
TACATATTCA CCAATGGCAT CGAGAAGATG AGAGAGATCC TGATGTCCGA ACCTGGGAAG 
CTGCTTCAGA AGGTGAAAGT GTGGTTACAG GAGTACTGGA ACATCACTGA CCTCATGGCC 
ATCCTCATCT TCTCCATCGG GATGGTGCTT CGTCTGCAGG ACCCGCCGCT GATGAGCTAC 
GGCAGGGTCA TTTACTGCGT CAATATCATT TATTGGTACA TACGACTCCT CGACATCTTC 
GGAGTGAACA AATACCTGGG TCCATACGTC ATGATGATCG GCAAGATGAT GATTGACATG 
ATGTATTTTG TGATCATCAT GTTGGTGGTG CTGATGAGTT TCGGTGTGGC GCGTCAGGCC 
ATCCTTAATC CCAATGAAGA CCCATCCTGG ATGCTGGCAC GCAATATCTT CTTCATGCCA 
TACTGGATGA TCTACGGAGA GGTGTTTGCT GACCAAATCG ACCCTCCTTG TGGTCAGAAC 
ATCACCACAG AAGAAGGAGT TGTCATGCCT CTTCCACCCT GCAAAACTGG AGCTTGGATT 
GTCCCTGCCA TAATGGCCTG CTACCTGCTG GTGGCCAACA TCCTGCTAGT TAACTTGCTC 
ATTGCTGTCT TTAATAACAC TTTCTTTGAA GTCAAGTCCA TTTCCAACCA GGTGTGGAAG 
TTTCAGAGGT ATCAGCTGAT CATGACCTTC CATGAACGCC CGGTCCTTCC TCCTCCACTC 
ATCATCTTCA GCCACATAAC TATGGTTCTC AAACACCTGT GCTGTCGCTG GAGGAAGCAC 
GATGAGGATG AACGGGACTA TGGGCTGAAG CTTTTCATCA CTGAAGATGA ACTGAAGAAA 
GTGCATGACT TTGAAGAGCA GTGCATGGAG GAGTATTTCA GAGAGAAAGA TGACCGCTTC 
AATTCCTCCA ATGACGAAAG GATACGTGTG ACATCTGAAA GGGTGGAGAA CATGGCGATG 
CGATTAGAGG AGGTGAATGA GAGGGAACAT TTTATGAAAG CGTCCTTGCA GACTGTAGAC 
ATCCGGCTCG CCCAGATGGA GGAGATGATC GGCCGCATTG CTGTGGCCCT AGAGAGAGTT 
GCTGGAATGG ACAGAGGCGA AGTTAACAAA GCCCGGTCCA GGACATCATC AGACTGCACT 
GATACCAATT ACATCCTGCG CCAAAGCAGT TTCAACAGCC AAGAAGGCAA TTCCTACAGG 
CTACAGGAGT CTCTGGAGCA GGGGGGCGAC GAGTCCATTT CTCCTACTTC CCCAACCGCC 
CTGGCTCCCC GTGTCCGCAG TCACTCCTTC TACGTGAGCC ACTCTTCCAA AGACAGGAGT 
GGGGCGGACC GTGGAGAGGG CTTCTTTAAA GACAAGTTGT TCAGCCTCCA CCGGGCCAAC 
AGCTCACAGT CTGTGTCCTC AGGAGCAGGT CCCAAAGAGT CCAAGCCAAC CCCTTTGAAT 
ACTTTATCCG TGCAGCAGCA GCTCCGCCCT TCTTCCTGCA TAGACATCTA TGTTTCTGCC 
TCAGAGGACG TCCCGCCCAC TGAGAGCTTC TTGGATTCGG TTCGGACAGT TCCAACTCTG 
GCTCGAGACT CATCTCTTCA TTCGGAGATC ATGGAGGCGG TGCTCTCAGG GGGACGGGAC 
TGTAGTGGAA GGGCAGGCGG CAGCGAAAGA CAGTCGGATG GTACAGTCCT GTTTGAGGAC 
AGCGCCGCCG CTGACCTATC GCTCTGCTCT GCCCACCTGC TGCCGGATTC CTTGCCCCCC 
TGGGACCTGG ACCCCTCACC GCCACCATCA GCTGGTGTAC TAGAGCGGTC CAAAAGTAGT 
CGCTTCCTGT CTACAGCAGG GCCTCTGTTT CTGGACGAGC CTCATTTGGT TAAGTCACAC 
AGTCTGATGT TCACATCACG TGGGTACTAT GGTGGCATGG GTGTGCAGGT GAAGGCGGCC 
GAATACACCA GCATCACAGA TTGCATCGAC ACCCGTTGCG TCTCAACCCC TTACCCTGTG 
CCAGAGCGGT CAGACTCACC TGGAGGATCT TTCACCTTCG ATAAGCCTCA AGACCTTGGC 
GTCTCGCACC CTGAGAGAGA CGCTGAGCTC AGCCACGCTG AGTCTGACTT AGAGGAGCCA 
GCAGAGGGTT CGGGAGACAC ATGTAAGGTG GGTCAGAGTA GCAGTAGTGG GGTAATAGGT 
GCAGACCTGG GCCTGGGCCT CGCACTAGGC CCGTTTTGCT CACCCATCTC TAGACTAGAG 
CGAGCAAACA GCTGCTCCTC TTCAGAGGAG TCGCACTCCA ACATCTACTC TCGAAAGAGC 
TTCTCAATAA GCGAGAGAAT GGACAAGGGC CGGGGGAGCT CCAGGAACCC TTTCCAGAAG 
GCCAGAACCG GGGCGAGGCT GGAGGGTAAG ACAGACTCAC TGTCTATGAG GAAAATGGCC 
AAACCGTCAG CTTTCAGAAG TTTTGACAGC AGACACAACT ACACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm3 ( XM_005171929.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171986.1 Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X11, mRNA.

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CloneID	ODa10544
Clone ID Related Accession (Same CDS sequence)	XM_005171929.3 , XM_005171929.4
Accession Version	XM_005171929.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily M member 3 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171929.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAAAA GAAAATGGGA GGGGAGGAGA GAAGGCTTCA AGGTGAGCTC CACGAGGGAG 
GAGGCACAGG GGCCTGGACG CCCCTCCACC AGAGGGCGCT TCTCCGAATC TTGGAAGAGA 
CTGAGCTCCA GACAGGGGTC TACCAAGAGG TCAGGACTGG CCTCCCAGCA AGCTGCGGCA 
CAGAAATCAT GGATAGAAAG AGCGTTCAGC AAGAGAGAAT GTGTGCACAT TATTGTAAGC 
ACCAAAGACC ACCATAGGTG CTGTTGTGGC CGTCTAATTG GTCAACATGT TGGCCTGCCC 
CCCAGCATCT CATCCAATCA GAATGAGAAG TCAGAACGCG TGCCAAAGAA TGACAGCTTG 
TCAGAGAAGT GGTCCATCAG CAAGCACACC CAGCTCAGTC CCACCGACGC TTTTGGGACC 
ATCGAGTTCC AGGGAGGAGG ACACTCTAAT AAAGCAATGT ATGTACGAGT GTCTTACGAC 
ACAAAACCAG ATTTACTGCT GCATCTGATG ACAAAGGAGT GGCAACTGGA GCTTCCCAAG 
CTGCTCATCT CTGTTCACGG AGGACTTCAA AACTTTGAGT TGCAGCCCAA ACTAAAGCAA 
GTCTTCGGCA AAGGTCTTAT AAAGGCTGCA ATGACTACCG GAGCCTGGAT CTTCACTGGA 
GGAGTTAATA CTGGAGTCAT CAGACATGTT GGTGATGCTC TGAAAGATCA TGCTTCCAAA 
TCAAGAGGGA AGATCTGCAC TATCGGCATT GCCCCCTGGG GGATTGTGGA GAACCAGGAA 
GACCTGGTGG GCAAAGATGT GGTCCGGCCA TACCAGACGA TGTCTAACCC TCTTAGCAAG 
CTAACAGTAC TCAACAGCCT ACACTCACAC TTCATTTTGG CTGATAATGG CACCACCGGA 
AAGTACGGAG CAGAAGTCAA ACTGCGCAGG CAGCTGGAGA AGCACATCTC TCTGCAAAAG 
ATTAACACAC GGATTGGACA GGGTGTTCCA GTAGTGGCTC TGATAGTGGA GGGGGGTCCG 
AATGTCATCT CTATCGTCCT GGAGTACCTA AGAGACACCC CTCCGGTGCC TGTTGTGGTG 
TGTGACGGCA GCGGTCGAGC CTCTGACATC CTGGCCTTTG GGCACAAGTA CTCAGAGGAG 
GGCGGAATCA TAAACGAATC TTTGAGAGAC CAGTTATTAG TCACCATTCA GAAGACGTTC 
ACATACAGCC GGACGCAGGC CCAGCATCTC TTCATCATTC TGATGGAGTG CATGAAGAAG 
AAAGAACTGA TCACAGTGTT TCGGATGGGA TCAGAGGGAC ACCAGGACAT CGATCTAGCC 
ATTCTAACAG CTCTTCTGAA AGGAGCGAAT GCATCAGCTC CAGATCAGCT AAGTCTGGCT 
TTGGCCTGGA ACAGAGTTGA CATCGCCCGG AGCCAGATCT TCATTTACGG ACAGCAGTGG 
CCTGTTGGCT CTCTGGAGCA GTCTATGCTG GACGCGCTGG TGCTGGACCG AGTGGACTTT 
GTAAAGCTGT TGATTGAGAA CGGAGTGAGC ATGCACCGTT TCCTGACACT CTCCAGACTT 
GAAGAGCTTT ATAATACGAG GCACGGACCA TCCAACACCT TGTACCACCT TGTCAGAGAT 
GTGAAAAAGC AAGAATATCC AGGGTTCAGT TGGATCTACC TCAAGGGTAA CCTGCCCCCC 
GACTACCGCA TTAGCCTGAT TGACATCGGA TTGGTTATTG AATACCTCAT GGGTGGAGCC 
TATCGCTGCA ATTACACCAG GAAGAGATTT CGGACCCTCT ATCACAACCT CTTTGGACCC 
AAAAGACCAA AAGCCCTGAA GCTGCTTGGA ATGGAGGATG ACATGCCCAT TAGACGAGGG 
CGTCAGAAGA CTACGCGGAA GCGAGAGGAG GAAGTAGATA TTGACCTGGA TGACCCAGAA 
ATCAATCATT TCCCTTTCCC ATTCCATGAG CTGATGGTTT GGGCTGTGCT GATGAAACGG 
CAGAAGATGG CACTGTTCTT CTGGCAGCAT GGAGAGGAGG CCATGGCTAA AGCGCTGGTG 
GCCTGCAAGC TCTGTAAGGC AATGGCTCAC GAAGCTTCTG AGAACGACAT GGTCGATGAC 
ATTTCACAGG AGCTCAATCA AAACTCAAGG GAGTTTGGTC AGCTGGCTGT TGAGCTGCTC 
GATCAGTCCT ATAAGCAGGA TGAACAGATG GCTATGAAGC TGTTGACCTA CGAGCTGAAG 
AACTGGAGTA ATGCCACCTG TCTGCAGCTG GCAGTGGCTG CCAAGCACCG AGACTTCATC 
GCCCACACCT GTAGCCAGAT GCTGCTCACA GACATGTGGA TGGGACGTCT GCGCATGCGC 
AAGAACTCTG GCCTGAAGGT TATCTTGGGC CTGCTTCTCC CCCCTTCCAT ATTGAGTCTG 
GAGTTTAAAA ATAAAGACGA GATGTCATAC ATGCCTCAGG ATCAGGACAC ATATCTGCAG 
GAGAAGGATG TGGATGAGCC CGAGAAACAA GCTAAGGAGA AAGAGGAGGA GGACATGGAG 
TTCACAGCTA TGTTGGGAAA CGTCAGTTCA GAAGCATCGC GCAAGAAACA GGTGGAGGAG 
GTTCAGAACC GGCATCGGCT CATTCCATTG GGTCGCAAAA TATATGAGTT TTACAACGCC 
CCTATCGTCA AGTTCTGGTT TCACACGCTG GCGTATGTAG GATACTTGAT GCTCTTCAAC 
TATATTGTGC TGGTGAAGAT GGACCTGTGG CCTTCTCCTC AGGAGTGGAT TGTTATTGCC 
TACATATTCA CCAATGGCAT CGAGAAGATG AGAGAGATCC TGATGTCCGA ACCTGGGAAG 
CTGCTTCAGA AGGTGAAAGT GTGGTTACAG GAGTACTGGA ACATCACTGA CCTCATGGCC 
ATCCTCATCT TCTCCATCGG GATGGTGCTT CGTCTGCAGG ACCCGCCGCT GATGAGCTAC 
GGCAGGGTCA TTTACTGCGT CAATATCATT TATTGGTACA TACGACTCCT CGACATCTTC 
GGAGTGAACA AATACCTGGG TCCATACGTC ATGATGATCG GCAAGATGAT GATTGACATG 
ATGTATTTTG TGATCATCAT GTTGGTGGTG CTGATGAGTT TCGGTGTGGC GCGTCAGGCC 
ATCCTTAATC CCAATGAAGA CCCATCCTGG ATGCTGGCAC GCAATATCTT CTTCATGCCA 
TACTGGATGA TCTACGGAGA GGTGTTTGCT GACCAAATCG ACCCTCCTTG TGGTCAGAAC 
ATCACCACAG AAGAAGGAGT TGTCATGCCT CTTCCACCCT GCAAAACTGG AGCTTGGATT 
GTCCCTGCCA TAATGGCCTG CTACCTGCTG GTGGCCAACA TCCTGCTAGT TAACTTGCTC 
ATTGCTGTCT TTAATAACAC TTTCTTTGAA GTCAAGTCCA TTTCCAACCA GGTGTGGAAG 
TTTCAGAGGT ATCAGCTGAT CATGACCTTC CATGAACGCC CGGTCCTTCC TCCTCCACTC 
ATCATCTTCA GCCACATAAC TATGGTTCTC AAACACCTGT GCTGTCGCTG GAGGAAGCAC 
GATGAGGATG AACGGGACTA TGGGCTGAAG CTTTTCATCA CTGAAGATGA ACTGAAGAAA 
GTGCATGACT TTGAAGAGCA GTGCATGGAG GAGTATTTCA GAGAGAAAGA TGACCGCTTC 
AATTCCTCCA ATGACGAAAG GATACGTGTG ACATCTGAAA GGGTGGAGAA CATGGCGATG 
CGATTAGAGG AGGTGAATGA GAGGGAACAT TTTATGAAAG CGTCCTTGCA GACTGTAGAC 
ATCCGGCTCG CCCAGATGGA GGAGATGATC GGCCGCATTG CTGTGGCCCT AGAGAGAGTT 
GCTGGAATGG ACAGAGGCGA AGTTAACAAA GCCCGGTCCA GGACATCATC AGACTGCACT 
GATACCAATT ACATCCTGCG CCAAAGCAGT TTCAACAGCC AAGAAGGCAA TTCCTACAGG 
CTACAGGAGT CTCTGGAGCA GGGGGGCGAC GAGTCCATTT CTCCTACTTC CCCAACCGCC 
CTGGCTCCCC GTGTCCGCAG TCACTCCTTC TACGTGAGCC ACTCTTCCAA AGACAGGAGT 
GGGGCGGACC GTGGAGAGGG CTTCTTTAAA GACAAGTTGT TCAGCCTCCA CCGGGCCAAC 
AGCTCACAGT CTGTGTCCTC AGGAGCAGGT CCCAAAGAGT CCAAGCCAAC CCCTTTGAAT 
ACTTTATCCG TGCAGCAGCA GCTCCGCCCT TCTTCCTGCA TAGACATCTA TGTTTCTGCC 
TCAGAGGACG TCCCGCCCAC TGAGAGCTTC TTGGATTCGG TTCGGACAGT TCCAACTCTG 
GCTCGAGACT CATCTCTTCA TTCGGAGATC ATGGAGGCGG TGCTCTCAGG GGGACGGGAC 
TGTAGTGGAA GGGCAGGCGG CAGCGAAAGA CAGTCGGATG GTACAGTCCT GTTTGAGGAC 
AGCGCCGCCG CTGACCTATC GCTCTGCTCT GCCCACCTGC TGCCGGATTC CTTGCCCCCC 
TGGGACCTGG ACCCCTCACC GCCACCATCA GCTGGTGTAC TAGAGCGGTC CAAAAGTAGT 
CGCTTCCTGT CTACAGCAGG GCCTCTGTTT CTGGACGAGC CTCATTTGGT TAAGTCACAC 
AGTCTGATGT TCACATCACG TGGGTACTAT GGTGGCATGG GTGTGCAGGT GAAGGCGGCC 
GAATACACCA GCATCACAGA TTGCATCGAC ACCCGTTGCG TCTCAACCCC TTACCCTGTG 
CCAGAGCGGT CAGACTCACC TGGAGGATCT TTCACCTTCG ATAAGCCTCA AGACCTTGGC 
GTCTCGCACC CTGAGAGAGA CGCTGAGCTC AGCCACGCTG AGTCTGACTT AGAGGAGCCA 
GCAGAGGGTT CGGGAGACAC ATGTAAGGTG GGTCAGAGTA GCAGTAGTGG GGTAATAGGT 
GCAGACCTGG GCCTGGGCCT CGCACTAGGC CCGTTTTGCT CACCCATCTC TAGACTAGAG 
CGAGCAAACA GCTGCTCCTC TTCAGAGGAG TCGCACTCCA ACATCTACTC TCGAAAGAGC 
TTCTCAATAA GCGAGAGAAT GGACAAGGGC CGGGGGAGCT CCAGGAACCC TTTCCAGAAG 
GCCAGAACCG GGGCGAGGCT GGAGGGTAAG ACAGACTCAC TGTCTATGAG GAAAATGGCC 
AAACCGTCAG CTTTCAGAAG TTTTGACAGC AGACACAACT ACACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171986.1
CDS	378..5585
Translation	MTKRKWEGRREGFKVSSTREEAQGPGRPSTRGRFSESWKRLSSRQGSTKRSGLASQQAAAQKSWIERAFSKRECVHIIVSTKDHHRCCCGRLIGQHVGLPPSISSNQNEKSERVPKNDSLSEKWSISKHTQLSPTDAFGTIEFQGGGHSNKAMYVRVSYDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLVGKDVVRPYQTMSNPLSKLTVLNSLHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGIINESLRDQLLVTIQKTFTYSRTQAQHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQSMLDALVLDRVDFVKLLIENGVSMHRFLTLSRLEELYNTRHGPSNTLYHLVRDVKKQEYPGFSWIYLKGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDMPIRRGRQKTTRKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNQNSREFGQLAVELLDQSYKQDEQMAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGLLLPPSILSLEFKNKDEMSYMPQDQDTYLQEKDVDEPEKQAKEKEEEDMEFTAMLGNVSSEASRKKQVEEVQNRHRLIPLGRKIYEFYNAPIVKFWFHTLAYVGYLMLFNYIVLVKMDLWPSPQEWIVIAYIFTNGIEKMREILMSEPGKLLQKVKVWLQEYWNITDLMAILIFSIGMVLRLQDPPLMSYGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDPPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHITMVLKHLCCRWRKHDEDERDYGLKLFITEDELKKVHDFEEQCMEEYFREKDDRFNSSNDERIRVTSERVENMAMRLEEVNEREHFMKASLQTVDIRLAQMEEMIGRIAVALERVAGMDRGEVNKARSRTSSDCTDTNYILRQSSFNSQEGNSYRLQESLEQGGDESISPTSPTALAPRVRSHSFYVSHSSKDRSGADRGEGFFKDKLFSLHRANSSQSVSSGAGPKESKPTPLNTLSVQQQLRPSSCIDIYVSASEDVPPTESFLDSVRTVPTLARDSSLHSEIMEAVLSGGRDCSGRAGGSERQSDGTVLFEDSAAADLSLCSAHLLPDSLPPWDLDPSPPPSAGVLERSKSSRFLSTAGPLFLDEPHLVKSHSLMFTSRGYYGGMGVQVKAAEYTSITDCIDTRCVSTPYPVPERSDSPGGSFTFDKPQDLGVSHPERDAELSHAESDLEEPAEGSGDTCKVGQSSSSGVIGADLGLGLALGPFCSPISRLERANSCSSSEESHSNIYSRKSFSISERMDKGRGSSRNPFQKARTGARLEGKTDSLSMRKMAKPSAFRSFDSRHNYT

Target ORF information:

RefSeq Version	XM_005171929.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171929.4

ORF Insert Sequence:

ATGACAAAAA GAAAATGGGA GGGGAGGAGA GAAGGCTTCA AGGTGAGCTC CACGAGGGAG 
GAGGCACAGG GGCCTGGACG CCCCTCCACC AGAGGGCGCT TCTCCGAATC TTGGAAGAGA 
CTGAGCTCCA GACAGGGGTC TACCAAGAGG TCAGGACTGG CCTCCCAGCA AGCTGCGGCA 
CAGAAATCAT GGATAGAAAG AGCGTTCAGC AAGAGAGAAT GTGTGCACAT TATTGTAAGC 
ACCAAAGACC ACCATAGGTG CTGTTGTGGC CGTCTAATTG GTCAACATGT TGGCCTGCCC 
CCCAGCATCT CATCCAATCA GAATGAGAAG TCAGAACGCG TGCCAAAGAA TGACAGCTTG 
TCAGAGAAGT GGTCCATCAG CAAGCACACC CAGCTCAGTC CCACCGACGC TTTTGGGACC 
ATCGAGTTCC AGGGAGGAGG ACACTCTAAT AAAGCAATGT ATGTACGAGT GTCTTACGAC 
ACAAAACCAG ATTTACTGCT GCATCTGATG ACAAAGGAGT GGCAACTGGA GCTTCCCAAG 
CTGCTCATCT CTGTTCACGG AGGACTTCAA AACTTTGAGT TGCAGCCCAA ACTAAAGCAA 
GTCTTCGGCA AAGGTCTTAT AAAGGCTGCA ATGACTACCG GAGCCTGGAT CTTCACTGGA 
GGAGTTAATA CTGGAGTCAT CAGACATGTT GGTGATGCTC TGAAAGATCA TGCTTCCAAA 
TCAAGAGGGA AGATCTGCAC TATCGGCATT GCCCCCTGGG GGATTGTGGA GAACCAGGAA 
GACCTGGTGG GCAAAGATGT GGTCCGGCCA TACCAGACGA TGTCTAACCC TCTTAGCAAG 
CTAACAGTAC TCAACAGCCT ACACTCACAC TTCATTTTGG CTGATAATGG CACCACCGGA 
AAGTACGGAG CAGAAGTCAA ACTGCGCAGG CAGCTGGAGA AGCACATCTC TCTGCAAAAG 
ATTAACACAC GGATTGGACA GGGTGTTCCA GTAGTGGCTC TGATAGTGGA GGGGGGTCCG 
AATGTCATCT CTATCGTCCT GGAGTACCTA AGAGACACCC CTCCGGTGCC TGTTGTGGTG 
TGTGACGGCA GCGGTCGAGC CTCTGACATC CTGGCCTTTG GGCACAAGTA CTCAGAGGAG 
GGCGGAATCA TAAACGAATC TTTGAGAGAC CAGTTATTAG TCACCATTCA GAAGACGTTC 
ACATACAGCC GGACGCAGGC CCAGCATCTC TTCATCATTC TGATGGAGTG CATGAAGAAG 
AAAGAACTGA TCACAGTGTT TCGGATGGGA TCAGAGGGAC ACCAGGACAT CGATCTAGCC 
ATTCTAACAG CTCTTCTGAA AGGAGCGAAT GCATCAGCTC CAGATCAGCT AAGTCTGGCT 
TTGGCCTGGA ACAGAGTTGA CATCGCCCGG AGCCAGATCT TCATTTACGG ACAGCAGTGG 
CCTGTTGGCT CTCTGGAGCA GTCTATGCTG GACGCGCTGG TGCTGGACCG AGTGGACTTT 
GTAAAGCTGT TGATTGAGAA CGGAGTGAGC ATGCACCGTT TCCTGACACT CTCCAGACTT 
GAAGAGCTTT ATAATACGAG GCACGGACCA TCCAACACCT TGTACCACCT TGTCAGAGAT 
GTGAAAAAGC AAGAATATCC AGGGTTCAGT TGGATCTACC TCAAGGGTAA CCTGCCCCCC 
GACTACCGCA TTAGCCTGAT TGACATCGGA TTGGTTATTG AATACCTCAT GGGTGGAGCC 
TATCGCTGCA ATTACACCAG GAAGAGATTT CGGACCCTCT ATCACAACCT CTTTGGACCC 
AAAAGACCAA AAGCCCTGAA GCTGCTTGGA ATGGAGGATG ACATGCCCAT TAGACGAGGG 
CGTCAGAAGA CTACGCGGAA GCGAGAGGAG GAAGTAGATA TTGACCTGGA TGACCCAGAA 
ATCAATCATT TCCCTTTCCC ATTCCATGAG CTGATGGTTT GGGCTGTGCT GATGAAACGG 
CAGAAGATGG CACTGTTCTT CTGGCAGCAT GGAGAGGAGG CCATGGCTAA AGCGCTGGTG 
GCCTGCAAGC TCTGTAAGGC AATGGCTCAC GAAGCTTCTG AGAACGACAT GGTCGATGAC 
ATTTCACAGG AGCTCAATCA AAACTCAAGG GAGTTTGGTC AGCTGGCTGT TGAGCTGCTC 
GATCAGTCCT ATAAGCAGGA TGAACAGATG GCTATGAAGC TGTTGACCTA CGAGCTGAAG 
AACTGGAGTA ATGCCACCTG TCTGCAGCTG GCAGTGGCTG CCAAGCACCG AGACTTCATC 
GCCCACACCT GTAGCCAGAT GCTGCTCACA GACATGTGGA TGGGACGTCT GCGCATGCGC 
AAGAACTCTG GCCTGAAGGT TATCTTGGGC CTGCTTCTCC CCCCTTCCAT ATTGAGTCTG 
GAGTTTAAAA ATAAAGACGA GATGTCATAC ATGCCTCAGG ATCAGGACAC ATATCTGCAG 
GAGAAGGATG TGGATGAGCC CGAGAAACAA GCTAAGGAGA AAGAGGAGGA GGACATGGAG 
TTCACAGCTA TGTTGGGAAA CGTCAGTTCA GAAGCATCGC GCAAGAAACA GGTGGAGGAG 
GTTCAGAACC GGCATCGGCT CATTCCATTG GGTCGCAAAA TATATGAGTT TTACAACGCC 
CCTATCGTCA AGTTCTGGTT TCACACGCTG GCGTATGTAG GATACTTGAT GCTCTTCAAC 
TATATTGTGC TGGTGAAGAT GGACCTGTGG CCTTCTCCTC AGGAGTGGAT TGTTATTGCC 
TACATATTCA CCAATGGCAT CGAGAAGATG AGAGAGATCC TGATGTCCGA ACCTGGGAAG 
CTGCTTCAGA AGGTGAAAGT GTGGTTACAG GAGTACTGGA ACATCACTGA CCTCATGGCC 
ATCCTCATCT TCTCCATCGG GATGGTGCTT CGTCTGCAGG ACCCGCCGCT GATGAGCTAC 
GGCAGGGTCA TTTACTGCGT CAATATCATT TATTGGTACA TACGACTCCT CGACATCTTC 
GGAGTGAACA AATACCTGGG TCCATACGTC ATGATGATCG GCAAGATGAT GATTGACATG 
ATGTATTTTG TGATCATCAT GTTGGTGGTG CTGATGAGTT TCGGTGTGGC GCGTCAGGCC 
ATCCTTAATC CCAATGAAGA CCCATCCTGG ATGCTGGCAC GCAATATCTT CTTCATGCCA 
TACTGGATGA TCTACGGAGA GGTGTTTGCT GACCAAATCG ACCCTCCTTG TGGTCAGAAC 
ATCACCACAG AAGAAGGAGT TGTCATGCCT CTTCCACCCT GCAAAACTGG AGCTTGGATT 
GTCCCTGCCA TAATGGCCTG CTACCTGCTG GTGGCCAACA TCCTGCTAGT TAACTTGCTC 
ATTGCTGTCT TTAATAACAC TTTCTTTGAA GTCAAGTCCA TTTCCAACCA GGTGTGGAAG 
TTTCAGAGGT ATCAGCTGAT CATGACCTTC CATGAACGCC CGGTCCTTCC TCCTCCACTC 
ATCATCTTCA GCCACATAAC TATGGTTCTC AAACACCTGT GCTGTCGCTG GAGGAAGCAC 
GATGAGGATG AACGGGACTA TGGGCTGAAG CTTTTCATCA CTGAAGATGA ACTGAAGAAA 
GTGCATGACT TTGAAGAGCA GTGCATGGAG GAGTATTTCA GAGAGAAAGA TGACCGCTTC 
AATTCCTCCA ATGACGAAAG GATACGTGTG ACATCTGAAA GGGTGGAGAA CATGGCGATG 
CGATTAGAGG AGGTGAATGA GAGGGAACAT TTTATGAAAG CGTCCTTGCA GACTGTAGAC 
ATCCGGCTCG CCCAGATGGA GGAGATGATC GGCCGCATTG CTGTGGCCCT AGAGAGAGTT 
GCTGGAATGG ACAGAGGCGA AGTTAACAAA GCCCGGTCCA GGACATCATC AGACTGCACT 
GATACCAATT ACATCCTGCG CCAAAGCAGT TTCAACAGCC AAGAAGGCAA TTCCTACAGG 
CTACAGGAGT CTCTGGAGCA GGGGGGCGAC GAGTCCATTT CTCCTACTTC CCCAACCGCC 
CTGGCTCCCC GTGTCCGCAG TCACTCCTTC TACGTGAGCC ACTCTTCCAA AGACAGGAGT 
GGGGCGGACC GTGGAGAGGG CTTCTTTAAA GACAAGTTGT TCAGCCTCCA CCGGGCCAAC 
AGCTCACAGT CTGTGTCCTC AGGAGCAGGT CCCAAAGAGT CCAAGCCAAC CCCTTTGAAT 
ACTTTATCCG TGCAGCAGCA GCTCCGCCCT TCTTCCTGCA TAGACATCTA TGTTTCTGCC 
TCAGAGGACG TCCCGCCCAC TGAGAGCTTC TTGGATTCGG TTCGGACAGT TCCAACTCTG 
GCTCGAGACT CATCTCTTCA TTCGGAGATC ATGGAGGCGG TGCTCTCAGG GGGACGGGAC 
TGTAGTGGAA GGGCAGGCGG CAGCGAAAGA CAGTCGGATG GTACAGTCCT GTTTGAGGAC 
AGCGCCGCCG CTGACCTATC GCTCTGCTCT GCCCACCTGC TGCCGGATTC CTTGCCCCCC 
TGGGACCTGG ACCCCTCACC GCCACCATCA GCTGGTGTAC TAGAGCGGTC CAAAAGTAGT 
CGCTTCCTGT CTACAGCAGG GCCTCTGTTT CTGGACGAGC CTCATTTGGT TAAGTCACAC 
AGTCTGATGT TCACATCACG TGGGTACTAT GGTGGCATGG GTGTGCAGGT GAAGGCGGCC 
GAATACACCA GCATCACAGA TTGCATCGAC ACCCGTTGCG TCTCAACCCC TTACCCTGTG 
CCAGAGCGGT CAGACTCACC TGGAGGATCT TTCACCTTCG ATAAGCCTCA AGACCTTGGC 
GTCTCGCACC CTGAGAGAGA CGCTGAGCTC AGCCACGCTG AGTCTGACTT AGAGGAGCCA 
GCAGAGGGTT CGGGAGACAC ATGTAAGGTG GGTCAGAGTA GCAGTAGTGG GGTAATAGGT 
GCAGACCTGG GCCTGGGCCT CGCACTAGGC CCGTTTTGCT CACCCATCTC TAGACTAGAG 
CGAGCAAACA GCTGCTCCTC TTCAGAGGAG TCGCACTCCA ACATCTACTC TCGAAAGAGC 
TTCTCAATAA GCGAGAGAAT GGACAAGGGC CGGGGGAGCT CCAGGAACCC TTTCCAGAAG 
GCCAGAACCG GGGCGAGGCT GGAGGGTAAG ACAGACTCAC TGTCTATGAG GAAAATGGCC 
AAACCGTCAG CTTTCAGAAG TTTTGACAGC AGACACAACT ACACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm3 ( XM_689075.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_694167.5 Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X8, mRNA.

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CloneID	ODa10546
Clone ID Related Accession (Same CDS sequence)	XM_689075.7 , XM_689075.8
Accession Version	XM_689075.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transient receptor potential cation channel subfamily M member 3 isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_689075.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGCC CTTTTCCGGA TGGCGGGAAC GTCCGGCCGG GAGCGATGAA CCAGCTGGAT 
GCTCCTCGGC CACTCAACTG GACCATACGG AAACTGTGCC ATGCAGCCTT CCTCCCGTCT 
GTGCGCCTGC TTAAGGCACA GAAATCATGG ATAGAAAGAG CGTTCAGCAA GAGAGAATGT 
GTGCACATTA TTGTAAGCAC CAAAGACCAC CATAGGTGCT GTTGTGGCCG TCTAATTGGT 
CAACATGTTG GCCTGCCCCC CAGCATCTCA TCCAATCAGA ATGAGAAGTC AGAACGCGTG 
CCAAAGAATG ACAGCTTGTC AGAGAAGTGG TCCATCAGCA AGCACACCCA GCTCAGTCCC 
ACCGACGCTT TTGGGACCAT CGAGTTCCAG GGAGGAGGAC ACTCTAATAA AGCAATGTAT 
GTACGAGTGT CTTACGACAC AAAACCAGAT TTACTGCTGC ATCTGATGAC AAAGGAGTGG 
CAACTGGAGC TTCCCAAGCT GCTCATCTCT GTTCACGGAG GACTTCAAAA CTTTGAGTTG 
CAGCCCAAAC TAAAGCAAGT CTTCGGCAAA GGTCTTATAA AGGCTGCAAT GACTACCGGA 
GCCTGGATCT TCACTGGAGG AGTTAATACT GGAGTCATCA GACATGTTGG TGATGCTCTG 
AAAGATCATG CTTCCAAATC AAGAGGGAAG ATCTGCACTA TCGGCATTGC CCCCTGGGGG 
ATTGTGGAGA ACCAGGAAGA CCTGGTGGGC AAAGATGTGG TCCGGCCATA CCAGACGATG 
TCTAACCCTC TTAGCAAGCT AACAGTACTC AACAGCCTAC ACTCACACTT CATTTTGGCT 
GATAATGGCA CCACCGGAAA GTACGGAGCA GAAGTCAAAC TGCGCAGGCA GCTGGAGAAG 
CACATCTCTC TGCAAAAGAT TAACACACGG ATTGGACAGG GTGTTCCAGT AGTGGCTCTG 
ATAGTGGAGG GGGGTCCGAA TGTCATCTCT ATCGTCCTGG AGTACCTAAG AGACACCCCT 
CCGGTGCCTG TTGTGGTGTG TGACGGCAGC GGTCGAGCCT CTGACATCCT GGCCTTTGGG 
CACAAGTACT CAGAGGAGGG CGGAATCATA AACGAATCTT TGAGAGACCA GTTATTAGTC 
ACCATTCAGA AGACGTTCAC ATACAGCCGG ACGCAGGCCC AGCATCTCTT CATCATTCTG 
ATGGAGTGCA TGAAGAAGAA AGAACTGATC ACAGTGTTTC GGATGGGATC AGAGGGACAC 
CAGGACATCG ATCTAGCCAT TCTAACAGCT CTTCTGAAAG GAGCGAATGC ATCAGCTCCA 
GATCAGCTAA GTCTGGCTTT GGCCTGGAAC AGAGTTGACA TCGCCCGGAG CCAGATCTTC 
ATTTACGGAC AGCAGTGGCC TGTTGGCTCT CTGGAGCAGT CTATGCTGGA CGCGCTGGTG 
CTGGACCGAG TGGACTTTGT AAAGCTGTTG ATTGAGAACG GAGTGAGCAT GCACCGTTTC 
CTGACACTCT CCAGACTTGA AGAGCTTTAT AATACGAGGC ACGGACCATC CAACACCTTG 
TACCACCTTG TCAGAGATGT GAAAAAGGGT AACCTGCCCC CCGACTACCG CATTAGCCTG 
ATTGACATCG GATTGGTTAT TGAATACCTC ATGGGTGGAG CCTATCGCTG CAATTACACC 
AGGAAGAGAT TTCGGACCCT CTATCACAAC CTCTTTGGAC CCAAAAGACC AAAAGCCCTG 
AAGCTGCTTG GAATGGAGGA TGACATGCCC ATTAGACGAG GGCGTCAGAA GACTACGCGG 
AAGCGAGAGG AGGAAGTAGA TATTGACCTG GATGACCCAG AAATCAATCA TTTCCCTTTC 
CCATTCCATG AGCTGATGGT TTGGGCTGTG CTGATGAAAC GGCAGAAGAT GGCACTGTTC 
TTCTGGCAGC ATGGAGAGGA GGCCATGGCT AAAGCGCTGG TGGCCTGCAA GCTCTGTAAG 
GCAATGGCTC ACGAAGCTTC TGAGAACGAC ATGGTCGATG ACATTTCACA GGAGCTCAAT 
CAAAACTCAA GGGAGTTTGG TCAGCTGGCT GTTGAGCTGC TCGATCAGTC CTATAAGCAG 
GATGAACAGA TGGCTATGAA GCTGTTGACC TACGAGCTGA AGAACTGGAG TAATGCCACC 
TGTCTGCAGC TGGCAGTGGC TGCCAAGCAC CGAGACTTCA TCGCCCACAC CTGTAGCCAG 
ATGCTGCTCA CAGACATGTG GATGGGACGT CTGCGCATGC GCAAGAACTC TGGCCTGAAG 
GTTATCTTGG GCCTGCTTCT CCCCCCTTCC ATATTGAGTC TGGAGTTTAA AAATAAAGAC 
GAGATGTCAT ACATGCCTCA GGATCAGGAC ACATATCTGC AGGAGAAGGA TGTGGATGAG 
CCCGAGAAAC AAGCTAAGGA GAAAGAGGAG GAGGACATGG AGTTCACAGC TATGTTGGGA 
AACGTCAGTT CAGAAGCATC GCGCAAGAAA CAGGTGGAGG AGGTTCAGAA CCGGCATCGG 
CTCATTCCAT TGGGTCGCAA AATATATGAG TTTTACAACG CCCCTATCGT CAAGTTCTGG 
TTTCACACGC TGGCGTATGT AGGATACTTG ATGCTCTTCA ACTATATTGT GCTGGTGAAG 
ATGGACCTGT GGCCTTCTCC TCAGGAGTGG ATTGTTATTG CCTACATATT CACCAATGGC 
ATCGAGAAGA TGAGAGAGAT CCTGATGTCC GAACCTGGGA AGCTGCTTCA GAAGGTGAAA 
GTGTGGTTAC AGGAGTACTG GAACATCACT GACCTCATGG CCATCCTCAT CTTCTCCATC 
GGGATGGTGC TTCGTCTGCA GGACCCGCCG CTGATGAGCT ACGGCAGGGT CATTTACTGC 
GTCAATATCA TTTATTGGTA CATACGACTC CTCGACATCT TCGGAGTGAA CAAATACCTG 
GGTCCATACG TCATGATGAT CGGCAAGATG ATGATTGACA TGATGTATTT TGTGATCATC 
ATGTTGGTGG TGCTGATGAG TTTCGGTGTG GCGCGTCAGG CCATCCTTAA TCCCAATGAA 
GACCCATCCT GGATGCTGGC ACGCAATATC TTCTTCATGC CATACTGGAT GATCTACGGA 
GAGGTGTTTG CTGACCAAAT CGACCCTCCT TGTGGTCAGA ACATCACCAC AGAAGAAGGA 
GTTGTCATGC CTCTTCCACC CTGCAAAACT GGAGCTTGGA TTGTCCCTGC CATAATGGCC 
TGCTACCTGC TGGTGGCCAA CATCCTGCTA GTTAACTTGC TCATTGCTGT CTTTAATAAC 
ACTTTCTTTG AAGTCAAGTC CATTTCCAAC CAGGTGTGGA AGTTTCAGAG GTATCAGCTG 
ATCATGACCT TCCATGAACG CCCGGTCCTT CCTCCTCCAC TCATCATCTT CAGCCACATA 
ACTATGGTTC TCAAACACCT GTGCTGTCGC TGGAGGAAGC ACGATGAGGA TGAACGGGAC 
TATGGGCTGA AGCTTTTCAT CACTGAAGAT GAACTGAAGA AAGTGCATGA CTTTGAAGAG 
CAGTGCATGG AGGAGTATTT CAGAGAGAAA GATGACCGCT TCAATTCCTC CAATGACGAA 
AGGATACGTG TGACATCTGA AAGGGTGGAG AACATGGCGA TGCGATTAGA GGAGGTGAAT 
GAGAGGGAAC ATTTTATGAA AGCGTCCTTG CAGACTGTAG ACATCCGGCT CGCCCAGATG 
GAGGAGATGA TCGGCCGCAT TGCTGTGGCC CTAGAGAGAG TTGCTGGAAT GGACAGAGGC 
GAAGTTAACA AAGCCCGGTC CAGGACATCA TCAGACTGCA CTGATACCAA TTACATCCTG 
CGCCAAAGCA GTTTCAACAG CCAAGAAGGC AATTCCTACA GGCTACAGGA GTCTCTGGAG 
CAGGGGGGCG ACGAGTCCAT TTCTCCTACT TCCCCAACCG CCCTGGCTCC CCGTGTCCGC 
AGTCACTCCT TCTACGTGAG CCACTCTTCC AAAGACAGGA GTGGGGCGGA CCGTGGAGAG 
GGCTTCTTTA AAGACAAGTT GTTCAGCCTC CACCGGGCCA ACAGCTCACA GTCTGTGTCC 
TCAGGAGCAG GTCCCAAAGA GTCCAAGCCA ACCCCTTTGA ATACTTTATC CGTGCAGCAG 
CAGCTCCGCC CTTCTTCCTG CATAGACATC TATGTTTCTG CCTCAGAGGA CGTCCCGCCC 
ACTGAGAGCT TCTTGGATTC GGTTCGGACA GTTCCAACTC TGGCTCGAGA CTCATCTCTT 
CATTCGGAGA TCATGGAGGC GGTGCTCTCA GGGGGACGGG ACTGTAGTGG AAGGGCAGGC 
GGCAGCGAAA GACAGTCGGA TGGTACAGTC CTGTTTGAGG ACAGCGCCGC CGCTGACCTA 
TCGCTCTGCT CTGCCCACCT GCTGCCGGAT TCCTTGCCCC CCTGGGACCT GGACCCCTCA 
CCGCCACCAT CAGCTGGTGT ACTAGAGCGG TCCAAAAGTA GTCGCTTCCT GTCTACAGCA 
GGGCCTCTGT TTCTGGACGA GCCTCATTTG GTTAAGTCAC ACAGTCTGAT GTTCACATCA 
CGTGGGTACT ATGGTGGCAT GGGTGTGCAG GTGAAGGCGG CCGAATACAC CAGCATCACA 
GATTGCATCG ACACCCGTTG CGTCTCAACC CCTTACCCTG TGCCAGAGCG GTCAGACTCA 
CCTGGAGGAT CTTTCACCTT CGATAAGCCT CAAGACCTTG GCGTCTCGCA CCCTGAGAGA 
GACGCTGAGC TCAGCCACGC TGAGTCTGAC TTAGAGGAGC CAGCAGAGGG TTCGGGAGAC 
ACATGTAAGG TGGGTCAGAG TAGCAGTAGT GGGGTAATAG GTGCAGACCT GGGCCTGGGC 
CTCGCACTAG GCCCGTTTTG CTCACCCATC TCTAGACTAG AGCGAGCAAA CAGCTGCTCC 
TCTTCAGAGG AGTCGCACTC CAACATCTAC TCTCGAAAGA GCTTCTCAAT AAGCGAGAGA 
ATGGACAAGG GCCGGGGGAG CTCCAGGAAC CCTTTCCAGA AGGCCAGAAC CGGGGCGAGG 
CTGGAGGGTA AGACAGACTC ACTGTCTATG AGGAAAATGG CCAAACCGTC AGCTTTCAGA 
AGTTTTGACA GCAGACACAA CTACACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_694167.5
CDS	375..5504
Translation	MWGPFPDGGNVRPGAMNQLDAPRPLNWTIRKLCHAAFLPSVRLLKAQKSWIERAFSKRECVHIIVSTKDHHRCCCGRLIGQHVGLPPSISSNQNEKSERVPKNDSLSEKWSISKHTQLSPTDAFGTIEFQGGGHSNKAMYVRVSYDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLVGKDVVRPYQTMSNPLSKLTVLNSLHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGIINESLRDQLLVTIQKTFTYSRTQAQHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQSMLDALVLDRVDFVKLLIENGVSMHRFLTLSRLEELYNTRHGPSNTLYHLVRDVKKGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDMPIRRGRQKTTRKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNQNSREFGQLAVELLDQSYKQDEQMAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGLLLPPSILSLEFKNKDEMSYMPQDQDTYLQEKDVDEPEKQAKEKEEEDMEFTAMLGNVSSEASRKKQVEEVQNRHRLIPLGRKIYEFYNAPIVKFWFHTLAYVGYLMLFNYIVLVKMDLWPSPQEWIVIAYIFTNGIEKMREILMSEPGKLLQKVKVWLQEYWNITDLMAILIFSIGMVLRLQDPPLMSYGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDPPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHITMVLKHLCCRWRKHDEDERDYGLKLFITEDELKKVHDFEEQCMEEYFREKDDRFNSSNDERIRVTSERVENMAMRLEEVNEREHFMKASLQTVDIRLAQMEEMIGRIAVALERVAGMDRGEVNKARSRTSSDCTDTNYILRQSSFNSQEGNSYRLQESLEQGGDESISPTSPTALAPRVRSHSFYVSHSSKDRSGADRGEGFFKDKLFSLHRANSSQSVSSGAGPKESKPTPLNTLSVQQQLRPSSCIDIYVSASEDVPPTESFLDSVRTVPTLARDSSLHSEIMEAVLSGGRDCSGRAGGSERQSDGTVLFEDSAAADLSLCSAHLLPDSLPPWDLDPSPPPSAGVLERSKSSRFLSTAGPLFLDEPHLVKSHSLMFTSRGYYGGMGVQVKAAEYTSITDCIDTRCVSTPYPVPERSDSPGGSFTFDKPQDLGVSHPERDAELSHAESDLEEPAEGSGDTCKVGQSSSSGVIGADLGLGLALGPFCSPISRLERANSCSSSEESHSNIYSRKSFSISERMDKGRGSSRNPFQKARTGARLEGKTDSLSMRKMAKPSAFRSFDSRHNYT

Target ORF information:

RefSeq Version	XM_689075.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_689075.7

ORF Insert Sequence:

ATGTGGGGCC CTTTTCCGGA TGGCGGGAAC GTCCGGCCGG GAGCGATGAA CCAGCTGGAT 
GCTCCTCGGC CACTCAACTG GACCATACGG AAACTGTGCC ATGCAGCCTT CCTCCCGTCT 
GTGCGCCTGC TTAAGGCACA GAAATCATGG ATAGAAAGAG CGTTCAGCAA GAGAGAATGT 
GTGCACATTA TTGTAAGCAC CAAAGACCAC CATAGGTGCT GTTGTGGCCG TCTAATTGGT 
CAACATGTTG GCCTGCCCCC CAGCATCTCA TCCAATCAGA ATGAGAAGTC AGAACGCGTG 
CCAAAGAATG ACAGCTTGTC AGAGAAGTGG TCCATCAGCA AGCACACCCA GCTCAGTCCC 
ACCGACGCTT TTGGGACCAT CGAGTTCCAG GGAGGAGGAC ACTCTAATAA AGCAATGTAT 
GTACGAGTGT CTTACGACAC AAAACCAGAT TTACTGCTGC ATCTGATGAC AAAGGAGTGG 
CAACTGGAGC TTCCCAAGCT GCTCATCTCT GTTCACGGAG GACTTCAAAA CTTTGAGTTG 
CAGCCCAAAC TAAAGCAAGT CTTCGGCAAA GGTCTTATAA AGGCTGCAAT GACTACCGGA 
GCCTGGATCT TCACTGGAGG AGTTAATACT GGAGTCATCA GACATGTTGG TGATGCTCTG 
AAAGATCATG CTTCCAAATC AAGAGGGAAG ATCTGCACTA TCGGCATTGC CCCCTGGGGG 
ATTGTGGAGA ACCAGGAAGA CCTGGTGGGC AAAGATGTGG TCCGGCCATA CCAGACGATG 
TCTAACCCTC TTAGCAAGCT AACAGTACTC AACAGCCTAC ACTCACACTT CATTTTGGCT 
GATAATGGCA CCACCGGAAA GTACGGAGCA GAAGTCAAAC TGCGCAGGCA GCTGGAGAAG 
CACATCTCTC TGCAAAAGAT TAACACACGG ATTGGACAGG GTGTTCCAGT AGTGGCTCTG 
ATAGTGGAGG GGGGTCCGAA TGTCATCTCT ATCGTCCTGG AGTACCTAAG AGACACCCCT 
CCGGTGCCTG TTGTGGTGTG TGACGGCAGC GGTCGAGCCT CTGACATCCT GGCCTTTGGG 
CACAAGTACT CAGAGGAGGG CGGAATCATA AACGAATCTT TGAGAGACCA GTTATTAGTC 
ACCATTCAGA AGACGTTCAC ATACAGCCGG ACGCAGGCCC AGCATCTCTT CATCATTCTG 
ATGGAGTGCA TGAAGAAGAA AGAACTGATC ACAGTGTTTC GGATGGGATC AGAGGGACAC 
CAGGACATCG ATCTAGCCAT TCTAACAGCT CTTCTGAAAG GAGCGAATGC ATCAGCTCCA 
GATCAGCTAA GTCTGGCTTT GGCCTGGAAC AGAGTTGACA TCGCCCGGAG CCAGATCTTC 
ATTTACGGAC AGCAGTGGCC TGTTGGCTCT CTGGAGCAGT CTATGCTGGA CGCGCTGGTG 
CTGGACCGAG TGGACTTTGT AAAGCTGTTG ATTGAGAACG GAGTGAGCAT GCACCGTTTC 
CTGACACTCT CCAGACTTGA AGAGCTTTAT AATACGAGGC ACGGACCATC CAACACCTTG 
TACCACCTTG TCAGAGATGT GAAAAAGGGT AACCTGCCCC CCGACTACCG CATTAGCCTG 
ATTGACATCG GATTGGTTAT TGAATACCTC ATGGGTGGAG CCTATCGCTG CAATTACACC 
AGGAAGAGAT TTCGGACCCT CTATCACAAC CTCTTTGGAC CCAAAAGACC AAAAGCCCTG 
AAGCTGCTTG GAATGGAGGA TGACATGCCC ATTAGACGAG GGCGTCAGAA GACTACGCGG 
AAGCGAGAGG AGGAAGTAGA TATTGACCTG GATGACCCAG AAATCAATCA TTTCCCTTTC 
CCATTCCATG AGCTGATGGT TTGGGCTGTG CTGATGAAAC GGCAGAAGAT GGCACTGTTC 
TTCTGGCAGC ATGGAGAGGA GGCCATGGCT AAAGCGCTGG TGGCCTGCAA GCTCTGTAAG 
GCAATGGCTC ACGAAGCTTC TGAGAACGAC ATGGTCGATG ACATTTCACA GGAGCTCAAT 
CAAAACTCAA GGGAGTTTGG TCAGCTGGCT GTTGAGCTGC TCGATCAGTC CTATAAGCAG 
GATGAACAGA TGGCTATGAA GCTGTTGACC TACGAGCTGA AGAACTGGAG TAATGCCACC 
TGTCTGCAGC TGGCAGTGGC TGCCAAGCAC CGAGACTTCA TCGCCCACAC CTGTAGCCAG 
ATGCTGCTCA CAGACATGTG GATGGGACGT CTGCGCATGC GCAAGAACTC TGGCCTGAAG 
GTTATCTTGG GCCTGCTTCT CCCCCCTTCC ATATTGAGTC TGGAGTTTAA AAATAAAGAC 
GAGATGTCAT ACATGCCTCA GGATCAGGAC ACATATCTGC AGGAGAAGGA TGTGGATGAG 
CCCGAGAAAC AAGCTAAGGA GAAAGAGGAG GAGGACATGG AGTTCACAGC TATGTTGGGA 
AACGTCAGTT CAGAAGCATC GCGCAAGAAA CAGGTGGAGG AGGTTCAGAA CCGGCATCGG 
CTCATTCCAT TGGGTCGCAA AATATATGAG TTTTACAACG CCCCTATCGT CAAGTTCTGG 
TTTCACACGC TGGCGTATGT AGGATACTTG ATGCTCTTCA ACTATATTGT GCTGGTGAAG 
ATGGACCTGT GGCCTTCTCC TCAGGAGTGG ATTGTTATTG CCTACATATT CACCAATGGC 
ATCGAGAAGA TGAGAGAGAT CCTGATGTCC GAACCTGGGA AGCTGCTTCA GAAGGTGAAA 
GTGTGGTTAC AGGAGTACTG GAACATCACT GACCTCATGG CCATCCTCAT CTTCTCCATC 
GGGATGGTGC TTCGTCTGCA GGACCCGCCG CTGATGAGCT ACGGCAGGGT CATTTACTGC 
GTCAATATCA TTTATTGGTA CATACGACTC CTCGACATCT TCGGAGTGAA CAAATACCTG 
GGTCCATACG TCATGATGAT CGGCAAGATG ATGATTGACA TGATGTATTT TGTGATCATC 
ATGTTGGTGG TGCTGATGAG TTTCGGTGTG GCGCGTCAGG CCATCCTTAA TCCCAATGAA 
GACCCATCCT GGATGCTGGC ACGCAATATC TTCTTCATGC CATACTGGAT GATCTACGGA 
GAGGTGTTTG CTGACCAAAT CGACCCTCCT TGTGGTCAGA ACATCACCAC AGAAGAAGGA 
GTTGTCATGC CTCTTCCACC CTGCAAAACT GGAGCTTGGA TTGTCCCTGC CATAATGGCC 
TGCTACCTGC TGGTGGCCAA CATCCTGCTA GTTAACTTGC TCATTGCTGT CTTTAATAAC 
ACTTTCTTTG AAGTCAAGTC CATTTCCAAC CAGGTGTGGA AGTTTCAGAG GTATCAGCTG 
ATCATGACCT TCCATGAACG CCCGGTCCTT CCTCCTCCAC TCATCATCTT CAGCCACATA 
ACTATGGTTC TCAAACACCT GTGCTGTCGC TGGAGGAAGC ACGATGAGGA TGAACGGGAC 
TATGGGCTGA AGCTTTTCAT CACTGAAGAT GAACTGAAGA AAGTGCATGA CTTTGAAGAG 
CAGTGCATGG AGGAGTATTT CAGAGAGAAA GATGACCGCT TCAATTCCTC CAATGACGAA 
AGGATACGTG TGACATCTGA AAGGGTGGAG AACATGGCGA TGCGATTAGA GGAGGTGAAT 
GAGAGGGAAC ATTTTATGAA AGCGTCCTTG CAGACTGTAG ACATCCGGCT CGCCCAGATG 
GAGGAGATGA TCGGCCGCAT TGCTGTGGCC CTAGAGAGAG TTGCTGGAAT GGACAGAGGC 
GAAGTTAACA AAGCCCGGTC CAGGACATCA TCAGACTGCA CTGATACCAA TTACATCCTG 
CGCCAAAGCA GTTTCAACAG CCAAGAAGGC AATTCCTACA GGCTACAGGA GTCTCTGGAG 
CAGGGGGGCG ACGAGTCCAT TTCTCCTACT TCCCCAACCG CCCTGGCTCC CCGTGTCCGC 
AGTCACTCCT TCTACGTGAG CCACTCTTCC AAAGACAGGA GTGGGGCGGA CCGTGGAGAG 
GGCTTCTTTA AAGACAAGTT GTTCAGCCTC CACCGGGCCA ACAGCTCACA GTCTGTGTCC 
TCAGGAGCAG GTCCCAAAGA GTCCAAGCCA ACCCCTTTGA ATACTTTATC CGTGCAGCAG 
CAGCTCCGCC CTTCTTCCTG CATAGACATC TATGTTTCTG CCTCAGAGGA CGTCCCGCCC 
ACTGAGAGCT TCTTGGATTC GGTTCGGACA GTTCCAACTC TGGCTCGAGA CTCATCTCTT 
CATTCGGAGA TCATGGAGGC GGTGCTCTCA GGGGGACGGG ACTGTAGTGG AAGGGCAGGC 
GGCAGCGAAA GACAGTCGGA TGGTACAGTC CTGTTTGAGG ACAGCGCCGC CGCTGACCTA 
TCGCTCTGCT CTGCCCACCT GCTGCCGGAT TCCTTGCCCC CCTGGGACCT GGACCCCTCA 
CCGCCACCAT CAGCTGGTGT ACTAGAGCGG TCCAAAAGTA GTCGCTTCCT GTCTACAGCA 
GGGCCTCTGT TTCTGGACGA GCCTCATTTG GTTAAGTCAC ACAGTCTGAT GTTCACATCA 
CGTGGGTACT ATGGTGGCAT GGGTGTGCAG GTGAAGGCGG CCGAATACAC CAGCATCACA 
GATTGCATCG ACACCCGTTG CGTCTCAACC CCTTACCCTG TGCCAGAGCG GTCAGACTCA 
CCTGGAGGAT CTTTCACCTT CGATAAGCCT CAAGACCTTG GCGTCTCGCA CCCTGAGAGA 
GACGCTGAGC TCAGCCACGC TGAGTCTGAC TTAGAGGAGC CAGCAGAGGG TTCGGGAGAC 
ACATGTAAGG TGGGTCAGAG TAGCAGTAGT GGGGTAATAG GTGCAGACCT GGGCCTGGGC 
CTCGCACTAG GCCCGTTTTG CTCACCCATC TCTAGACTAG AGCGAGCAAA CAGCTGCTCC 
TCTTCAGAGG AGTCGCACTC CAACATCTAC TCTCGAAAGA GCTTCTCAAT AAGCGAGAGA 
ATGGACAAGG GCCGGGGGAG CTCCAGGAAC CCTTTCCAGA AGGCCAGAAC CGGGGCGAGG 
CTGGAGGGTA AGACAGACTC ACTGTCTATG AGGAAAATGG CCAAACCGTC AGCTTTCAGA 
AGTTTTGACA GCAGACACAA CTACACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm3 ( XM_689075.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_694167.5 Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X14, mRNA.

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CloneID	ODa10546
Clone ID Related Accession (Same CDS sequence)	XM_689075.7 , XM_689075.8
Accession Version	XM_689075.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5130bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily M member 3 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_689075.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGCC CTTTTCCGGA TGGCGGGAAC GTCCGGCCGG GAGCGATGAA CCAGCTGGAT 
GCTCCTCGGC CACTCAACTG GACCATACGG AAACTGTGCC ATGCAGCCTT CCTCCCGTCT 
GTGCGCCTGC TTAAGGCACA GAAATCATGG ATAGAAAGAG CGTTCAGCAA GAGAGAATGT 
GTGCACATTA TTGTAAGCAC CAAAGACCAC CATAGGTGCT GTTGTGGCCG TCTAATTGGT 
CAACATGTTG GCCTGCCCCC CAGCATCTCA TCCAATCAGA ATGAGAAGTC AGAACGCGTG 
CCAAAGAATG ACAGCTTGTC AGAGAAGTGG TCCATCAGCA AGCACACCCA GCTCAGTCCC 
ACCGACGCTT TTGGGACCAT CGAGTTCCAG GGAGGAGGAC ACTCTAATAA AGCAATGTAT 
GTACGAGTGT CTTACGACAC AAAACCAGAT TTACTGCTGC ATCTGATGAC AAAGGAGTGG 
CAACTGGAGC TTCCCAAGCT GCTCATCTCT GTTCACGGAG GACTTCAAAA CTTTGAGTTG 
CAGCCCAAAC TAAAGCAAGT CTTCGGCAAA GGTCTTATAA AGGCTGCAAT GACTACCGGA 
GCCTGGATCT TCACTGGAGG AGTTAATACT GGAGTCATCA GACATGTTGG TGATGCTCTG 
AAAGATCATG CTTCCAAATC AAGAGGGAAG ATCTGCACTA TCGGCATTGC CCCCTGGGGG 
ATTGTGGAGA ACCAGGAAGA CCTGGTGGGC AAAGATGTGG TCCGGCCATA CCAGACGATG 
TCTAACCCTC TTAGCAAGCT AACAGTACTC AACAGCCTAC ACTCACACTT CATTTTGGCT 
GATAATGGCA CCACCGGAAA GTACGGAGCA GAAGTCAAAC TGCGCAGGCA GCTGGAGAAG 
CACATCTCTC TGCAAAAGAT TAACACACGG ATTGGACAGG GTGTTCCAGT AGTGGCTCTG 
ATAGTGGAGG GGGGTCCGAA TGTCATCTCT ATCGTCCTGG AGTACCTAAG AGACACCCCT 
CCGGTGCCTG TTGTGGTGTG TGACGGCAGC GGTCGAGCCT CTGACATCCT GGCCTTTGGG 
CACAAGTACT CAGAGGAGGG CGGAATCATA AACGAATCTT TGAGAGACCA GTTATTAGTC 
ACCATTCAGA AGACGTTCAC ATACAGCCGG ACGCAGGCCC AGCATCTCTT CATCATTCTG 
ATGGAGTGCA TGAAGAAGAA AGAACTGATC ACAGTGTTTC GGATGGGATC AGAGGGACAC 
CAGGACATCG ATCTAGCCAT TCTAACAGCT CTTCTGAAAG GAGCGAATGC ATCAGCTCCA 
GATCAGCTAA GTCTGGCTTT GGCCTGGAAC AGAGTTGACA TCGCCCGGAG CCAGATCTTC 
ATTTACGGAC AGCAGTGGCC TGTTGGCTCT CTGGAGCAGT CTATGCTGGA CGCGCTGGTG 
CTGGACCGAG TGGACTTTGT AAAGCTGTTG ATTGAGAACG GAGTGAGCAT GCACCGTTTC 
CTGACACTCT CCAGACTTGA AGAGCTTTAT AATACGAGGC ACGGACCATC CAACACCTTG 
TACCACCTTG TCAGAGATGT GAAAAAGGGT AACCTGCCCC CCGACTACCG CATTAGCCTG 
ATTGACATCG GATTGGTTAT TGAATACCTC ATGGGTGGAG CCTATCGCTG CAATTACACC 
AGGAAGAGAT TTCGGACCCT CTATCACAAC CTCTTTGGAC CCAAAAGACC AAAAGCCCTG 
AAGCTGCTTG GAATGGAGGA TGACATGCCC ATTAGACGAG GGCGTCAGAA GACTACGCGG 
AAGCGAGAGG AGGAAGTAGA TATTGACCTG GATGACCCAG AAATCAATCA TTTCCCTTTC 
CCATTCCATG AGCTGATGGT TTGGGCTGTG CTGATGAAAC GGCAGAAGAT GGCACTGTTC 
TTCTGGCAGC ATGGAGAGGA GGCCATGGCT AAAGCGCTGG TGGCCTGCAA GCTCTGTAAG 
GCAATGGCTC ACGAAGCTTC TGAGAACGAC ATGGTCGATG ACATTTCACA GGAGCTCAAT 
CAAAACTCAA GGGAGTTTGG TCAGCTGGCT GTTGAGCTGC TCGATCAGTC CTATAAGCAG 
GATGAACAGA TGGCTATGAA GCTGTTGACC TACGAGCTGA AGAACTGGAG TAATGCCACC 
TGTCTGCAGC TGGCAGTGGC TGCCAAGCAC CGAGACTTCA TCGCCCACAC CTGTAGCCAG 
ATGCTGCTCA CAGACATGTG GATGGGACGT CTGCGCATGC GCAAGAACTC TGGCCTGAAG 
GTTATCTTGG GCCTGCTTCT CCCCCCTTCC ATATTGAGTC TGGAGTTTAA AAATAAAGAC 
GAGATGTCAT ACATGCCTCA GGATCAGGAC ACATATCTGC AGGAGAAGGA TGTGGATGAG 
CCCGAGAAAC AAGCTAAGGA GAAAGAGGAG GAGGACATGG AGTTCACAGC TATGTTGGGA 
AACGTCAGTT CAGAAGCATC GCGCAAGAAA CAGGTGGAGG AGGTTCAGAA CCGGCATCGG 
CTCATTCCAT TGGGTCGCAA AATATATGAG TTTTACAACG CCCCTATCGT CAAGTTCTGG 
TTTCACACGC TGGCGTATGT AGGATACTTG ATGCTCTTCA ACTATATTGT GCTGGTGAAG 
ATGGACCTGT GGCCTTCTCC TCAGGAGTGG ATTGTTATTG CCTACATATT CACCAATGGC 
ATCGAGAAGA TGAGAGAGAT CCTGATGTCC GAACCTGGGA AGCTGCTTCA GAAGGTGAAA 
GTGTGGTTAC AGGAGTACTG GAACATCACT GACCTCATGG CCATCCTCAT CTTCTCCATC 
GGGATGGTGC TTCGTCTGCA GGACCCGCCG CTGATGAGCT ACGGCAGGGT CATTTACTGC 
GTCAATATCA TTTATTGGTA CATACGACTC CTCGACATCT TCGGAGTGAA CAAATACCTG 
GGTCCATACG TCATGATGAT CGGCAAGATG ATGATTGACA TGATGTATTT TGTGATCATC 
ATGTTGGTGG TGCTGATGAG TTTCGGTGTG GCGCGTCAGG CCATCCTTAA TCCCAATGAA 
GACCCATCCT GGATGCTGGC ACGCAATATC TTCTTCATGC CATACTGGAT GATCTACGGA 
GAGGTGTTTG CTGACCAAAT CGACCCTCCT TGTGGTCAGA ACATCACCAC AGAAGAAGGA 
GTTGTCATGC CTCTTCCACC CTGCAAAACT GGAGCTTGGA TTGTCCCTGC CATAATGGCC 
TGCTACCTGC TGGTGGCCAA CATCCTGCTA GTTAACTTGC TCATTGCTGT CTTTAATAAC 
ACTTTCTTTG AAGTCAAGTC CATTTCCAAC CAGGTGTGGA AGTTTCAGAG GTATCAGCTG 
ATCATGACCT TCCATGAACG CCCGGTCCTT CCTCCTCCAC TCATCATCTT CAGCCACATA 
ACTATGGTTC TCAAACACCT GTGCTGTCGC TGGAGGAAGC ACGATGAGGA TGAACGGGAC 
TATGGGCTGA AGCTTTTCAT CACTGAAGAT GAACTGAAGA AAGTGCATGA CTTTGAAGAG 
CAGTGCATGG AGGAGTATTT CAGAGAGAAA GATGACCGCT TCAATTCCTC CAATGACGAA 
AGGATACGTG TGACATCTGA AAGGGTGGAG AACATGGCGA TGCGATTAGA GGAGGTGAAT 
GAGAGGGAAC ATTTTATGAA AGCGTCCTTG CAGACTGTAG ACATCCGGCT CGCCCAGATG 
GAGGAGATGA TCGGCCGCAT TGCTGTGGCC CTAGAGAGAG TTGCTGGAAT GGACAGAGGC 
GAAGTTAACA AAGCCCGGTC CAGGACATCA TCAGACTGCA CTGATACCAA TTACATCCTG 
CGCCAAAGCA GTTTCAACAG CCAAGAAGGC AATTCCTACA GGCTACAGGA GTCTCTGGAG 
CAGGGGGGCG ACGAGTCCAT TTCTCCTACT TCCCCAACCG CCCTGGCTCC CCGTGTCCGC 
AGTCACTCCT TCTACGTGAG CCACTCTTCC AAAGACAGGA GTGGGGCGGA CCGTGGAGAG 
GGCTTCTTTA AAGACAAGTT GTTCAGCCTC CACCGGGCCA ACAGCTCACA GTCTGTGTCC 
TCAGGAGCAG GTCCCAAAGA GTCCAAGCCA ACCCCTTTGA ATACTTTATC CGTGCAGCAG 
CAGCTCCGCC CTTCTTCCTG CATAGACATC TATGTTTCTG CCTCAGAGGA CGTCCCGCCC 
ACTGAGAGCT TCTTGGATTC GGTTCGGACA GTTCCAACTC TGGCTCGAGA CTCATCTCTT 
CATTCGGAGA TCATGGAGGC GGTGCTCTCA GGGGGACGGG ACTGTAGTGG AAGGGCAGGC 
GGCAGCGAAA GACAGTCGGA TGGTACAGTC CTGTTTGAGG ACAGCGCCGC CGCTGACCTA 
TCGCTCTGCT CTGCCCACCT GCTGCCGGAT TCCTTGCCCC CCTGGGACCT GGACCCCTCA 
CCGCCACCAT CAGCTGGTGT ACTAGAGCGG TCCAAAAGTA GTCGCTTCCT GTCTACAGCA 
GGGCCTCTGT TTCTGGACGA GCCTCATTTG GTTAAGTCAC ACAGTCTGAT GTTCACATCA 
CGTGGGTACT ATGGTGGCAT GGGTGTGCAG GTGAAGGCGG CCGAATACAC CAGCATCACA 
GATTGCATCG ACACCCGTTG CGTCTCAACC CCTTACCCTG TGCCAGAGCG GTCAGACTCA 
CCTGGAGGAT CTTTCACCTT CGATAAGCCT CAAGACCTTG GCGTCTCGCA CCCTGAGAGA 
GACGCTGAGC TCAGCCACGC TGAGTCTGAC TTAGAGGAGC CAGCAGAGGG TTCGGGAGAC 
ACATGTAAGG TGGGTCAGAG TAGCAGTAGT GGGGTAATAG GTGCAGACCT GGGCCTGGGC 
CTCGCACTAG GCCCGTTTTG CTCACCCATC TCTAGACTAG AGCGAGCAAA CAGCTGCTCC 
TCTTCAGAGG AGTCGCACTC CAACATCTAC TCTCGAAAGA GCTTCTCAAT AAGCGAGAGA 
ATGGACAAGG GCCGGGGGAG CTCCAGGAAC CCTTTCCAGA AGGCCAGAAC CGGGGCGAGG 
CTGGAGGGTA AGACAGACTC ACTGTCTATG AGGAAAATGG CCAAACCGTC AGCTTTCAGA 
AGTTTTGACA GCAGACACAA CTACACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_694167.5
CDS	393..5522
Translation	MWGPFPDGGNVRPGAMNQLDAPRPLNWTIRKLCHAAFLPSVRLLKAQKSWIERAFSKRECVHIIVSTKDHHRCCCGRLIGQHVGLPPSISSNQNEKSERVPKNDSLSEKWSISKHTQLSPTDAFGTIEFQGGGHSNKAMYVRVSYDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLVGKDVVRPYQTMSNPLSKLTVLNSLHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGIINESLRDQLLVTIQKTFTYSRTQAQHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQSMLDALVLDRVDFVKLLIENGVSMHRFLTLSRLEELYNTRHGPSNTLYHLVRDVKKGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDMPIRRGRQKTTRKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNQNSREFGQLAVELLDQSYKQDEQMAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGLLLPPSILSLEFKNKDEMSYMPQDQDTYLQEKDVDEPEKQAKEKEEEDMEFTAMLGNVSSEASRKKQVEEVQNRHRLIPLGRKIYEFYNAPIVKFWFHTLAYVGYLMLFNYIVLVKMDLWPSPQEWIVIAYIFTNGIEKMREILMSEPGKLLQKVKVWLQEYWNITDLMAILIFSIGMVLRLQDPPLMSYGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDPPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHITMVLKHLCCRWRKHDEDERDYGLKLFITEDELKKVHDFEEQCMEEYFREKDDRFNSSNDERIRVTSERVENMAMRLEEVNEREHFMKASLQTVDIRLAQMEEMIGRIAVALERVAGMDRGEVNKARSRTSSDCTDTNYILRQSSFNSQEGNSYRLQESLEQGGDESISPTSPTALAPRVRSHSFYVSHSSKDRSGADRGEGFFKDKLFSLHRANSSQSVSSGAGPKESKPTPLNTLSVQQQLRPSSCIDIYVSASEDVPPTESFLDSVRTVPTLARDSSLHSEIMEAVLSGGRDCSGRAGGSERQSDGTVLFEDSAAADLSLCSAHLLPDSLPPWDLDPSPPPSAGVLERSKSSRFLSTAGPLFLDEPHLVKSHSLMFTSRGYYGGMGVQVKAAEYTSITDCIDTRCVSTPYPVPERSDSPGGSFTFDKPQDLGVSHPERDAELSHAESDLEEPAEGSGDTCKVGQSSSSGVIGADLGLGLALGPFCSPISRLERANSCSSSEESHSNIYSRKSFSISERMDKGRGSSRNPFQKARTGARLEGKTDSLSMRKMAKPSAFRSFDSRHNYT

Target ORF information:

RefSeq Version	XM_689075.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_689075.8

ORF Insert Sequence:

ATGTGGGGCC CTTTTCCGGA TGGCGGGAAC GTCCGGCCGG GAGCGATGAA CCAGCTGGAT 
GCTCCTCGGC CACTCAACTG GACCATACGG AAACTGTGCC ATGCAGCCTT CCTCCCGTCT 
GTGCGCCTGC TTAAGGCACA GAAATCATGG ATAGAAAGAG CGTTCAGCAA GAGAGAATGT 
GTGCACATTA TTGTAAGCAC CAAAGACCAC CATAGGTGCT GTTGTGGCCG TCTAATTGGT 
CAACATGTTG GCCTGCCCCC CAGCATCTCA TCCAATCAGA ATGAGAAGTC AGAACGCGTG 
CCAAAGAATG ACAGCTTGTC AGAGAAGTGG TCCATCAGCA AGCACACCCA GCTCAGTCCC 
ACCGACGCTT TTGGGACCAT CGAGTTCCAG GGAGGAGGAC ACTCTAATAA AGCAATGTAT 
GTACGAGTGT CTTACGACAC AAAACCAGAT TTACTGCTGC ATCTGATGAC AAAGGAGTGG 
CAACTGGAGC TTCCCAAGCT GCTCATCTCT GTTCACGGAG GACTTCAAAA CTTTGAGTTG 
CAGCCCAAAC TAAAGCAAGT CTTCGGCAAA GGTCTTATAA AGGCTGCAAT GACTACCGGA 
GCCTGGATCT TCACTGGAGG AGTTAATACT GGAGTCATCA GACATGTTGG TGATGCTCTG 
AAAGATCATG CTTCCAAATC AAGAGGGAAG ATCTGCACTA TCGGCATTGC CCCCTGGGGG 
ATTGTGGAGA ACCAGGAAGA CCTGGTGGGC AAAGATGTGG TCCGGCCATA CCAGACGATG 
TCTAACCCTC TTAGCAAGCT AACAGTACTC AACAGCCTAC ACTCACACTT CATTTTGGCT 
GATAATGGCA CCACCGGAAA GTACGGAGCA GAAGTCAAAC TGCGCAGGCA GCTGGAGAAG 
CACATCTCTC TGCAAAAGAT TAACACACGG ATTGGACAGG GTGTTCCAGT AGTGGCTCTG 
ATAGTGGAGG GGGGTCCGAA TGTCATCTCT ATCGTCCTGG AGTACCTAAG AGACACCCCT 
CCGGTGCCTG TTGTGGTGTG TGACGGCAGC GGTCGAGCCT CTGACATCCT GGCCTTTGGG 
CACAAGTACT CAGAGGAGGG CGGAATCATA AACGAATCTT TGAGAGACCA GTTATTAGTC 
ACCATTCAGA AGACGTTCAC ATACAGCCGG ACGCAGGCCC AGCATCTCTT CATCATTCTG 
ATGGAGTGCA TGAAGAAGAA AGAACTGATC ACAGTGTTTC GGATGGGATC AGAGGGACAC 
CAGGACATCG ATCTAGCCAT TCTAACAGCT CTTCTGAAAG GAGCGAATGC ATCAGCTCCA 
GATCAGCTAA GTCTGGCTTT GGCCTGGAAC AGAGTTGACA TCGCCCGGAG CCAGATCTTC 
ATTTACGGAC AGCAGTGGCC TGTTGGCTCT CTGGAGCAGT CTATGCTGGA CGCGCTGGTG 
CTGGACCGAG TGGACTTTGT AAAGCTGTTG ATTGAGAACG GAGTGAGCAT GCACCGTTTC 
CTGACACTCT CCAGACTTGA AGAGCTTTAT AATACGAGGC ACGGACCATC CAACACCTTG 
TACCACCTTG TCAGAGATGT GAAAAAGGGT AACCTGCCCC CCGACTACCG CATTAGCCTG 
ATTGACATCG GATTGGTTAT TGAATACCTC ATGGGTGGAG CCTATCGCTG CAATTACACC 
AGGAAGAGAT TTCGGACCCT CTATCACAAC CTCTTTGGAC CCAAAAGACC AAAAGCCCTG 
AAGCTGCTTG GAATGGAGGA TGACATGCCC ATTAGACGAG GGCGTCAGAA GACTACGCGG 
AAGCGAGAGG AGGAAGTAGA TATTGACCTG GATGACCCAG AAATCAATCA TTTCCCTTTC 
CCATTCCATG AGCTGATGGT TTGGGCTGTG CTGATGAAAC GGCAGAAGAT GGCACTGTTC 
TTCTGGCAGC ATGGAGAGGA GGCCATGGCT AAAGCGCTGG TGGCCTGCAA GCTCTGTAAG 
GCAATGGCTC ACGAAGCTTC TGAGAACGAC ATGGTCGATG ACATTTCACA GGAGCTCAAT 
CAAAACTCAA GGGAGTTTGG TCAGCTGGCT GTTGAGCTGC TCGATCAGTC CTATAAGCAG 
GATGAACAGA TGGCTATGAA GCTGTTGACC TACGAGCTGA AGAACTGGAG TAATGCCACC 
TGTCTGCAGC TGGCAGTGGC TGCCAAGCAC CGAGACTTCA TCGCCCACAC CTGTAGCCAG 
ATGCTGCTCA CAGACATGTG GATGGGACGT CTGCGCATGC GCAAGAACTC TGGCCTGAAG 
GTTATCTTGG GCCTGCTTCT CCCCCCTTCC ATATTGAGTC TGGAGTTTAA AAATAAAGAC 
GAGATGTCAT ACATGCCTCA GGATCAGGAC ACATATCTGC AGGAGAAGGA TGTGGATGAG 
CCCGAGAAAC AAGCTAAGGA GAAAGAGGAG GAGGACATGG AGTTCACAGC TATGTTGGGA 
AACGTCAGTT CAGAAGCATC GCGCAAGAAA CAGGTGGAGG AGGTTCAGAA CCGGCATCGG 
CTCATTCCAT TGGGTCGCAA AATATATGAG TTTTACAACG CCCCTATCGT CAAGTTCTGG 
TTTCACACGC TGGCGTATGT AGGATACTTG ATGCTCTTCA ACTATATTGT GCTGGTGAAG 
ATGGACCTGT GGCCTTCTCC TCAGGAGTGG ATTGTTATTG CCTACATATT CACCAATGGC 
ATCGAGAAGA TGAGAGAGAT CCTGATGTCC GAACCTGGGA AGCTGCTTCA GAAGGTGAAA 
GTGTGGTTAC AGGAGTACTG GAACATCACT GACCTCATGG CCATCCTCAT CTTCTCCATC 
GGGATGGTGC TTCGTCTGCA GGACCCGCCG CTGATGAGCT ACGGCAGGGT CATTTACTGC 
GTCAATATCA TTTATTGGTA CATACGACTC CTCGACATCT TCGGAGTGAA CAAATACCTG 
GGTCCATACG TCATGATGAT CGGCAAGATG ATGATTGACA TGATGTATTT TGTGATCATC 
ATGTTGGTGG TGCTGATGAG TTTCGGTGTG GCGCGTCAGG CCATCCTTAA TCCCAATGAA 
GACCCATCCT GGATGCTGGC ACGCAATATC TTCTTCATGC CATACTGGAT GATCTACGGA 
GAGGTGTTTG CTGACCAAAT CGACCCTCCT TGTGGTCAGA ACATCACCAC AGAAGAAGGA 
GTTGTCATGC CTCTTCCACC CTGCAAAACT GGAGCTTGGA TTGTCCCTGC CATAATGGCC 
TGCTACCTGC TGGTGGCCAA CATCCTGCTA GTTAACTTGC TCATTGCTGT CTTTAATAAC 
ACTTTCTTTG AAGTCAAGTC CATTTCCAAC CAGGTGTGGA AGTTTCAGAG GTATCAGCTG 
ATCATGACCT TCCATGAACG CCCGGTCCTT CCTCCTCCAC TCATCATCTT CAGCCACATA 
ACTATGGTTC TCAAACACCT GTGCTGTCGC TGGAGGAAGC ACGATGAGGA TGAACGGGAC 
TATGGGCTGA AGCTTTTCAT CACTGAAGAT GAACTGAAGA AAGTGCATGA CTTTGAAGAG 
CAGTGCATGG AGGAGTATTT CAGAGAGAAA GATGACCGCT TCAATTCCTC CAATGACGAA 
AGGATACGTG TGACATCTGA AAGGGTGGAG AACATGGCGA TGCGATTAGA GGAGGTGAAT 
GAGAGGGAAC ATTTTATGAA AGCGTCCTTG CAGACTGTAG ACATCCGGCT CGCCCAGATG 
GAGGAGATGA TCGGCCGCAT TGCTGTGGCC CTAGAGAGAG TTGCTGGAAT GGACAGAGGC 
GAAGTTAACA AAGCCCGGTC CAGGACATCA TCAGACTGCA CTGATACCAA TTACATCCTG 
CGCCAAAGCA GTTTCAACAG CCAAGAAGGC AATTCCTACA GGCTACAGGA GTCTCTGGAG 
CAGGGGGGCG ACGAGTCCAT TTCTCCTACT TCCCCAACCG CCCTGGCTCC CCGTGTCCGC 
AGTCACTCCT TCTACGTGAG CCACTCTTCC AAAGACAGGA GTGGGGCGGA CCGTGGAGAG 
GGCTTCTTTA AAGACAAGTT GTTCAGCCTC CACCGGGCCA ACAGCTCACA GTCTGTGTCC 
TCAGGAGCAG GTCCCAAAGA GTCCAAGCCA ACCCCTTTGA ATACTTTATC CGTGCAGCAG 
CAGCTCCGCC CTTCTTCCTG CATAGACATC TATGTTTCTG CCTCAGAGGA CGTCCCGCCC 
ACTGAGAGCT TCTTGGATTC GGTTCGGACA GTTCCAACTC TGGCTCGAGA CTCATCTCTT 
CATTCGGAGA TCATGGAGGC GGTGCTCTCA GGGGGACGGG ACTGTAGTGG AAGGGCAGGC 
GGCAGCGAAA GACAGTCGGA TGGTACAGTC CTGTTTGAGG ACAGCGCCGC CGCTGACCTA 
TCGCTCTGCT CTGCCCACCT GCTGCCGGAT TCCTTGCCCC CCTGGGACCT GGACCCCTCA 
CCGCCACCAT CAGCTGGTGT ACTAGAGCGG TCCAAAAGTA GTCGCTTCCT GTCTACAGCA 
GGGCCTCTGT TTCTGGACGA GCCTCATTTG GTTAAGTCAC ACAGTCTGAT GTTCACATCA 
CGTGGGTACT ATGGTGGCAT GGGTGTGCAG GTGAAGGCGG CCGAATACAC CAGCATCACA 
GATTGCATCG ACACCCGTTG CGTCTCAACC CCTTACCCTG TGCCAGAGCG GTCAGACTCA 
CCTGGAGGAT CTTTCACCTT CGATAAGCCT CAAGACCTTG GCGTCTCGCA CCCTGAGAGA 
GACGCTGAGC TCAGCCACGC TGAGTCTGAC TTAGAGGAGC CAGCAGAGGG TTCGGGAGAC 
ACATGTAAGG TGGGTCAGAG TAGCAGTAGT GGGGTAATAG GTGCAGACCT GGGCCTGGGC 
CTCGCACTAG GCCCGTTTTG CTCACCCATC TCTAGACTAG AGCGAGCAAA CAGCTGCTCC 
TCTTCAGAGG AGTCGCACTC CAACATCTAC TCTCGAAAGA GCTTCTCAAT AAGCGAGAGA 
ATGGACAAGG GCCGGGGGAG CTCCAGGAAC CCTTTCCAGA AGGCCAGAAC CGGGGCGAGG 
CTGGAGGGTA AGACAGACTC ACTGTCTATG AGGAAAATGG CCAAACCGTC AGCTTTCAGA 
AGTTTTGACA GCAGACACAA CTACACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm3 ( XM_005171931.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171988.1 Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X13, mRNA.

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CloneID	ODa10547
Clone ID Related Accession (Same CDS sequence)	XM_005171931.4 , XM_005171931.3
Accession Version	XM_005171931.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily M member 3 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005171931.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGCC CTTTTCCGGA TGGCGGGAAC GTCCGGCCGG GAGCGATGAA CCAGCTGGAT 
GCTCCTCGGC CACTCAACTG GACCATACGG AAACTGTGCC ATGCAGCCTT CCTCCCGTCT 
GTGCGCCTGC TTAAGGCACA GAAATCATGG ATAGAAAGAG CGTTCAGCAA GAGAGAATGT 
GTGCACATTA TTGTAAGCAC CAAAGACCAC CATAGGTGCT GTTGTGGCCG TCTAATTGGT 
CAACATGTTG GCCTGCCCCC CAGCATCTCA TCCAATCAGA ATGAGAAGTC AGAACGCGTG 
CCAAAGAATG ACAGCTTGTC AGAGAAGTGG TCCATCAGCA AGCACACCCA GCTCAGTCCC 
ACCGACGCTT TTGGGACCAT CGAGTTCCAG GGAGGAGGAC ACTCTAATAA AGCAATGTAT 
GTACGAGTGT CTTACGACAC AAAACCAGAT TTACTGCTGC ATCTGATGAC AAAGGAGTGG 
CAACTGGAGC TTCCCAAGCT GCTCATCTCT GTTCACGGAG GACTTCAAAA CTTTGAGTTG 
CAGCCCAAAC TAAAGCAAGT CTTCGGCAAA GGTCTTATAA AGGCTGCAAT GACTACCGGA 
GCCTGGATCT TCACTGGAGG AGTTAATACT GGAGTCATCA GACATGTTGG TGATGCTCTG 
AAAGATCATG CTTCCAAATC AAGAGGGAAG ATCTGCACTA TCGGCATTGC CCCCTGGGGG 
ATTGTGGAGA ACCAGGAAGA CCTGGTGGGC AAAGATGTGG TCCGGCCATA CCAGACGATG 
TCTAACCCTC TTAGCAAGCT AACAGTACTC AACAGCCTAC ACTCACACTT CATTTTGGCT 
GATAATGGCA CCACCGGAAA GTACGGAGCA GAAGTCAAAC TGCGCAGGCA GCTGGAGAAG 
CACATCTCTC TGCAAAAGAT TAACACACGG ATTGGACAGG GTGTTCCAGT AGTGGCTCTG 
ATAGTGGAGG GGGGTCCGAA TGTCATCTCT ATCGTCCTGG AGTACCTAAG AGACACCCCT 
CCGGTGCCTG TTGTGGTGTG TGACGGCAGC GGTCGAGCCT CTGACATCCT GGCCTTTGGG 
CACAAGTACT CAGAGGAGGG CGGAATCATA AACGAATCTT TGAGAGACCA GTTATTAGTC 
ACCATTCAGA AGACGTTCAC ATACAGCCGG ACGCAGGCCC AGCATCTCTT CATCATTCTG 
ATGGAGTGCA TGAAGAAGAA AGAACTGATC ACAGTGTTTC GGATGGGATC AGAGGGACAC 
CAGGACATCG ATCTAGCCAT TCTAACAGCT CTTCTGAAAG GAGCGAATGC ATCAGCTCCA 
GATCAGCTAA GTCTGGCTTT GGCCTGGAAC AGAGTTGACA TCGCCCGGAG CCAGATCTTC 
ATTTACGGAC AGCAGTGGCC TGTTGGCTCT CTGGAGCAGT CTATGCTGGA CGCGCTGGTG 
CTGGACCGAG TGGACTTTGT AAAGCTGTTG ATTGAGAACG GAGTGAGCAT GCACCGTTTC 
CTGACACTCT CCAGACTTGA AGAGCTTTAT AATACGAGGC ACGGACCATC CAACACCTTG 
TACCACCTTG TCAGAGATGT GAAAAAGCAA GAATATCCAG GGTTCAGTTG GATCTACCTC 
AAGGGTAACC TGCCCCCCGA CTACCGCATT AGCCTGATTG ACATCGGATT GGTTATTGAA 
TACCTCATGG GTGGAGCCTA TCGCTGCAAT TACACCAGGA AGAGATTTCG GACCCTCTAT 
CACAACCTCT TTGGACCCAA AAGACCAAAA GCCCTGAAGC TGCTTGGAAT GGAGGATGAC 
ATGCCCATTA GACGAGGGCG TCAGAAGACT ACGCGGAAGC GAGAGGAGGA AGTAGATATT 
GACCTGGATG ACCCAGAAAT CAATCATTTC CCTTTCCCAT TCCATGAGCT GATGGTTTGG 
GCTGTGCTGA TGAAACGGCA GAAGATGGCA CTGTTCTTCT GGCAGCATGG AGAGGAGGCC 
ATGGCTAAAG CGCTGGTGGC CTGCAAGCTC TGTAAGGCAA TGGCTCACGA AGCTTCTGAG 
AACGACATGG TCGATGACAT TTCACAGGAG CTCAATCAAA ACTCAAGGGA GTTTGGTCAG 
CTGGCTGTTG AGCTGCTCGA TCAGTCCTAT AAGCAGGATG AACAGATGGC TATGAAGCTG 
TTGACCTACG AGCTGAAGAA CTGGAGTAAT GCCACCTGTC TGCAGCTGGC AGTGGCTGCC 
AAGCACCGAG ACTTCATCGC CCACACCTGT AGCCAGATGC TGCTCACAGA CATGTGGATG 
GGACGTCTGC GCATGCGCAA GAACTCTGGC CTGAAGGTTA TCTTGGGCCT GCTTCTCCCC 
CCTTCCATAT TGAGTCTGGA GTTTAAAAAT AAAGACGAGA TGTCATACAT GCCTCAGGAT 
CAGGACACAT ATCTGCAGGA GAAGGATGTG GATGAGCCCG AGAAACAAGC TAAGGAGAAA 
GAGGAGGAGG ACATGGAGTT CACAGCTATG TTGGGAAACG TCAGTTCAGA AGCATCGCGC 
AAGAAACAGG TGGAGGAGGT TCAGAACCGG CATCGGCTCA TTCCATTGGG TCGCAAAATA 
TATGAGTTTT ACAACGCCCC TATCGTCAAG TTCTGGTTTC ACACGCTGGC GTATGTAGGA 
TACTTGATGC TCTTCAACTA TATTGTGCTG GTGAAGATGG ACCTGTGGCC TTCTCCTCAG 
GAGTGGATTG TTATTGCCTA CATATTCACC AATGGCATCG AGAAGATGAG AGAGATCCTG 
ATGTCCGAAC CTGGGAAGCT GCTTCAGAAG GTGAAAGTGT GGTTACAGGA GTACTGGAAC 
ATCACTGACC TCATGGCCAT CCTCATCTTC TCCATCGGGA TGGTGCTTCG TCTGCAGGAC 
CCGCCGCTGA TGAGCTACGG CAGGGTCATT TACTGCGTCA ATATCATTTA TTGGTACATA 
CGACTCCTCG ACATCTTCGG AGTGAACAAA TACCTGGGTC CATACGTCAT GATGATCGGC 
AAGATGATGA TTGACATGAT GTATTTTGTG ATCATCATGT TGGTGGTGCT GATGAGTTTC 
GGTGTGGCGC GTCAGGCCAT CCTTAATCCC AATGAAGACC CATCCTGGAT GCTGGCACGC 
AATATCTTCT TCATGCCATA CTGGATGATC TACGGAGAGG TGTTTGCTGA CCAAATCGAC 
CCTCCTTGTG GTCAGAACAT CACCACAGAA GAAGGAGTTG TCATGCCTCT TCCACCCTGC 
AAAACTGGAG CTTGGATTGT CCCTGCCATA ATGGCCTGCT ACCTGCTGGT GGCCAACATC 
CTGCTAGTTA ACTTGCTCAT TGCTGTCTTT AATAACACTT TCTTTGAAGT CAAGTCCATT 
TCCAACCAGG TGTGGAAGTT TCAGAGGTAT CAGCTGATCA TGACCTTCCA TGAACGCCCG 
GTCCTTCCTC CTCCACTCAT CATCTTCAGC CACATAACTA TGGTTCTCAA ACACCTGTGC 
TGTCGCTGGA GGAAGCACGA TGAGGATGAA CGGGACTATG GGCTGAAGCT TTTCATCACT 
GAAGATGAAC TGAAGAAAGT GCATGACTTT GAAGAGCAGT GCATGGAGGA GTATTTCAGA 
GAGAAAGATG ACCGCTTCAA TTCCTCCAAT GACGAAAGGA TACGTGTGAC ATCTGAAAGG 
GTGGAGAACA TGGCGATGCG ATTAGAGGAG GTGAATGAGA GGGAACATTT TATGAAAGCG 
TCCTTGCAGA CTGTAGACAT CCGGCTCGCC CAGATGGAGG AGATGATCGG CCGCATTGCT 
GTGGCCCTAG AGAGAGTTGC TGGAATGGAC AGAGGCGAAG TTAACAAAGC CCGGTCCAGG 
ACATCATCAG ACTGCACTGA TACCAATTAC ATCCTGCGCC AAAGCAGTTT CAACAGCCAA 
GAAGGCAATT CCTACAGGCT ACAGGAGTCT CTGGAGCAGG GGGGCGACGA GTCCATTTCT 
CCTACTTCCC CAACCGCCCT GGCTCCCCGT GTCCGCAGTC ACTCCTTCTA CGTGAGCCAC 
TCTTCCAAAG ACAGGAGTGG GGCGGACCGT GGAGAGGGCT TCTTTAAAGA CAAGTTGTTC 
AGCCTCCACC GGGCCAACAG CTCACAGTCT GTGTCCTCAG GAGCAGGTCC CAAAGAGTCC 
AAGCCAACCC CTTTGAATAC TTTATCCGTG CAGCAGCAGC TCCGCCCTTC TTCCTGCATA 
GACATCTATG TTTCTGCCTC AGAGGACGTC CCGCCCACTG AGAGCTTCTT GGATTCGGTT 
CGGACAGTTC CAACTCTGGC TCGAGACTCA TCTCTTCATT CGGAGATCAT GGAGGCGGTG 
CTCTCAGGGG GACGGGACTG TAGTGGAAGG GCAGGCGGCA GCGAAAGACA GTCGGATGGT 
ACAGTCCTGT TTGAGGACAG CGCCGCCGCT GACCTATCGC TCTGCTCTGC CCACCTGCTG 
CCGGATTCCT TGCCCCCCTG GGACCTGGAC CCCTCACCGC CACCATCAGC TGGTGTACTA 
GAGCGGTCCA AAAGTAGTCG CTTCCTGTCT ACAGCAGGGC CTCTGTTTCT GGACGAGCCT 
CATTTGGTTA AGTCACACAG TCTGATGTTC ACATCACGTG GGTACTATGG TGGCATGGGT 
GTGCAGGTGA AGGCGGCCGA ATACACCAGC ATCACAGATT GCATCGACAC CCGTTGCGTC 
TCAACCCCTT ACCCTGTGCC AGAGCGGTCA GACTCACCTG GAGGATCTTT CACCTTCGAT 
AAGCCTCAAG ACCTTGGCGT CTCGCACCCT GAGAGAGACG CTGAGCTCAG CCACGCTGAG 
TCTGACTTAG AGGAGCCAGC AGAGGGTTCG GGAGACACAT GTAAGGTGGG TCAGAGTAGC 
AGTAGTGGGG TAATAGGTGC AGACCTGGGC CTGGGCCTCG CACTAGGCCC GTTTTGCTCA 
CCCATCTCTA GACTAGAGCG AGCAAACAGC TGCTCCTCTT CAGAGGAGTC GCACTCCAAC 
ATCTACTCTC GAAAGAGCTT CTCAATAAGC GAGAGAATGG ACAAGGGCCG GGGGAGCTCC 
AGGAACCCTT TCCAGAAGGC CAGAACCGGG GCGAGGCTGG AGGGTAAGAC AGACTCACTG 
TCTATGAGGA AAATGGCCAA ACCGTCAGCT TTCAGAAGTT TTGACAGCAG ACACAACTAC 
ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171988.1
CDS	395..5560
Translation	MWGPFPDGGNVRPGAMNQLDAPRPLNWTIRKLCHAAFLPSVRLLKAQKSWIERAFSKRECVHIIVSTKDHHRCCCGRLIGQHVGLPPSISSNQNEKSERVPKNDSLSEKWSISKHTQLSPTDAFGTIEFQGGGHSNKAMYVRVSYDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLVGKDVVRPYQTMSNPLSKLTVLNSLHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGIINESLRDQLLVTIQKTFTYSRTQAQHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQSMLDALVLDRVDFVKLLIENGVSMHRFLTLSRLEELYNTRHGPSNTLYHLVRDVKKQEYPGFSWIYLKGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDMPIRRGRQKTTRKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNQNSREFGQLAVELLDQSYKQDEQMAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGLLLPPSILSLEFKNKDEMSYMPQDQDTYLQEKDVDEPEKQAKEKEEEDMEFTAMLGNVSSEASRKKQVEEVQNRHRLIPLGRKIYEFYNAPIVKFWFHTLAYVGYLMLFNYIVLVKMDLWPSPQEWIVIAYIFTNGIEKMREILMSEPGKLLQKVKVWLQEYWNITDLMAILIFSIGMVLRLQDPPLMSYGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDPPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHITMVLKHLCCRWRKHDEDERDYGLKLFITEDELKKVHDFEEQCMEEYFREKDDRFNSSNDERIRVTSERVENMAMRLEEVNEREHFMKASLQTVDIRLAQMEEMIGRIAVALERVAGMDRGEVNKARSRTSSDCTDTNYILRQSSFNSQEGNSYRLQESLEQGGDESISPTSPTALAPRVRSHSFYVSHSSKDRSGADRGEGFFKDKLFSLHRANSSQSVSSGAGPKESKPTPLNTLSVQQQLRPSSCIDIYVSASEDVPPTESFLDSVRTVPTLARDSSLHSEIMEAVLSGGRDCSGRAGGSERQSDGTVLFEDSAAADLSLCSAHLLPDSLPPWDLDPSPPPSAGVLERSKSSRFLSTAGPLFLDEPHLVKSHSLMFTSRGYYGGMGVQVKAAEYTSITDCIDTRCVSTPYPVPERSDSPGGSFTFDKPQDLGVSHPERDAELSHAESDLEEPAEGSGDTCKVGQSSSSGVIGADLGLGLALGPFCSPISRLERANSCSSSEESHSNIYSRKSFSISERMDKGRGSSRNPFQKARTGARLEGKTDSLSMRKMAKPSAFRSFDSRHNYT

Target ORF information:

RefSeq Version	XM_005171931.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171931.4

ORF Insert Sequence:

ATGTGGGGCC CTTTTCCGGA TGGCGGGAAC GTCCGGCCGG GAGCGATGAA CCAGCTGGAT 
GCTCCTCGGC CACTCAACTG GACCATACGG AAACTGTGCC ATGCAGCCTT CCTCCCGTCT 
GTGCGCCTGC TTAAGGCACA GAAATCATGG ATAGAAAGAG CGTTCAGCAA GAGAGAATGT 
GTGCACATTA TTGTAAGCAC CAAAGACCAC CATAGGTGCT GTTGTGGCCG TCTAATTGGT 
CAACATGTTG GCCTGCCCCC CAGCATCTCA TCCAATCAGA ATGAGAAGTC AGAACGCGTG 
CCAAAGAATG ACAGCTTGTC AGAGAAGTGG TCCATCAGCA AGCACACCCA GCTCAGTCCC 
ACCGACGCTT TTGGGACCAT CGAGTTCCAG GGAGGAGGAC ACTCTAATAA AGCAATGTAT 
GTACGAGTGT CTTACGACAC AAAACCAGAT TTACTGCTGC ATCTGATGAC AAAGGAGTGG 
CAACTGGAGC TTCCCAAGCT GCTCATCTCT GTTCACGGAG GACTTCAAAA CTTTGAGTTG 
CAGCCCAAAC TAAAGCAAGT CTTCGGCAAA GGTCTTATAA AGGCTGCAAT GACTACCGGA 
GCCTGGATCT TCACTGGAGG AGTTAATACT GGAGTCATCA GACATGTTGG TGATGCTCTG 
AAAGATCATG CTTCCAAATC AAGAGGGAAG ATCTGCACTA TCGGCATTGC CCCCTGGGGG 
ATTGTGGAGA ACCAGGAAGA CCTGGTGGGC AAAGATGTGG TCCGGCCATA CCAGACGATG 
TCTAACCCTC TTAGCAAGCT AACAGTACTC AACAGCCTAC ACTCACACTT CATTTTGGCT 
GATAATGGCA CCACCGGAAA GTACGGAGCA GAAGTCAAAC TGCGCAGGCA GCTGGAGAAG 
CACATCTCTC TGCAAAAGAT TAACACACGG ATTGGACAGG GTGTTCCAGT AGTGGCTCTG 
ATAGTGGAGG GGGGTCCGAA TGTCATCTCT ATCGTCCTGG AGTACCTAAG AGACACCCCT 
CCGGTGCCTG TTGTGGTGTG TGACGGCAGC GGTCGAGCCT CTGACATCCT GGCCTTTGGG 
CACAAGTACT CAGAGGAGGG CGGAATCATA AACGAATCTT TGAGAGACCA GTTATTAGTC 
ACCATTCAGA AGACGTTCAC ATACAGCCGG ACGCAGGCCC AGCATCTCTT CATCATTCTG 
ATGGAGTGCA TGAAGAAGAA AGAACTGATC ACAGTGTTTC GGATGGGATC AGAGGGACAC 
CAGGACATCG ATCTAGCCAT TCTAACAGCT CTTCTGAAAG GAGCGAATGC ATCAGCTCCA 
GATCAGCTAA GTCTGGCTTT GGCCTGGAAC AGAGTTGACA TCGCCCGGAG CCAGATCTTC 
ATTTACGGAC AGCAGTGGCC TGTTGGCTCT CTGGAGCAGT CTATGCTGGA CGCGCTGGTG 
CTGGACCGAG TGGACTTTGT AAAGCTGTTG ATTGAGAACG GAGTGAGCAT GCACCGTTTC 
CTGACACTCT CCAGACTTGA AGAGCTTTAT AATACGAGGC ACGGACCATC CAACACCTTG 
TACCACCTTG TCAGAGATGT GAAAAAGCAA GAATATCCAG GGTTCAGTTG GATCTACCTC 
AAGGGTAACC TGCCCCCCGA CTACCGCATT AGCCTGATTG ACATCGGATT GGTTATTGAA 
TACCTCATGG GTGGAGCCTA TCGCTGCAAT TACACCAGGA AGAGATTTCG GACCCTCTAT 
CACAACCTCT TTGGACCCAA AAGACCAAAA GCCCTGAAGC TGCTTGGAAT GGAGGATGAC 
ATGCCCATTA GACGAGGGCG TCAGAAGACT ACGCGGAAGC GAGAGGAGGA AGTAGATATT 
GACCTGGATG ACCCAGAAAT CAATCATTTC CCTTTCCCAT TCCATGAGCT GATGGTTTGG 
GCTGTGCTGA TGAAACGGCA GAAGATGGCA CTGTTCTTCT GGCAGCATGG AGAGGAGGCC 
ATGGCTAAAG CGCTGGTGGC CTGCAAGCTC TGTAAGGCAA TGGCTCACGA AGCTTCTGAG 
AACGACATGG TCGATGACAT TTCACAGGAG CTCAATCAAA ACTCAAGGGA GTTTGGTCAG 
CTGGCTGTTG AGCTGCTCGA TCAGTCCTAT AAGCAGGATG AACAGATGGC TATGAAGCTG 
TTGACCTACG AGCTGAAGAA CTGGAGTAAT GCCACCTGTC TGCAGCTGGC AGTGGCTGCC 
AAGCACCGAG ACTTCATCGC CCACACCTGT AGCCAGATGC TGCTCACAGA CATGTGGATG 
GGACGTCTGC GCATGCGCAA GAACTCTGGC CTGAAGGTTA TCTTGGGCCT GCTTCTCCCC 
CCTTCCATAT TGAGTCTGGA GTTTAAAAAT AAAGACGAGA TGTCATACAT GCCTCAGGAT 
CAGGACACAT ATCTGCAGGA GAAGGATGTG GATGAGCCCG AGAAACAAGC TAAGGAGAAA 
GAGGAGGAGG ACATGGAGTT CACAGCTATG TTGGGAAACG TCAGTTCAGA AGCATCGCGC 
AAGAAACAGG TGGAGGAGGT TCAGAACCGG CATCGGCTCA TTCCATTGGG TCGCAAAATA 
TATGAGTTTT ACAACGCCCC TATCGTCAAG TTCTGGTTTC ACACGCTGGC GTATGTAGGA 
TACTTGATGC TCTTCAACTA TATTGTGCTG GTGAAGATGG ACCTGTGGCC TTCTCCTCAG 
GAGTGGATTG TTATTGCCTA CATATTCACC AATGGCATCG AGAAGATGAG AGAGATCCTG 
ATGTCCGAAC CTGGGAAGCT GCTTCAGAAG GTGAAAGTGT GGTTACAGGA GTACTGGAAC 
ATCACTGACC TCATGGCCAT CCTCATCTTC TCCATCGGGA TGGTGCTTCG TCTGCAGGAC 
CCGCCGCTGA TGAGCTACGG CAGGGTCATT TACTGCGTCA ATATCATTTA TTGGTACATA 
CGACTCCTCG ACATCTTCGG AGTGAACAAA TACCTGGGTC CATACGTCAT GATGATCGGC 
AAGATGATGA TTGACATGAT GTATTTTGTG ATCATCATGT TGGTGGTGCT GATGAGTTTC 
GGTGTGGCGC GTCAGGCCAT CCTTAATCCC AATGAAGACC CATCCTGGAT GCTGGCACGC 
AATATCTTCT TCATGCCATA CTGGATGATC TACGGAGAGG TGTTTGCTGA CCAAATCGAC 
CCTCCTTGTG GTCAGAACAT CACCACAGAA GAAGGAGTTG TCATGCCTCT TCCACCCTGC 
AAAACTGGAG CTTGGATTGT CCCTGCCATA ATGGCCTGCT ACCTGCTGGT GGCCAACATC 
CTGCTAGTTA ACTTGCTCAT TGCTGTCTTT AATAACACTT TCTTTGAAGT CAAGTCCATT 
TCCAACCAGG TGTGGAAGTT TCAGAGGTAT CAGCTGATCA TGACCTTCCA TGAACGCCCG 
GTCCTTCCTC CTCCACTCAT CATCTTCAGC CACATAACTA TGGTTCTCAA ACACCTGTGC 
TGTCGCTGGA GGAAGCACGA TGAGGATGAA CGGGACTATG GGCTGAAGCT TTTCATCACT 
GAAGATGAAC TGAAGAAAGT GCATGACTTT GAAGAGCAGT GCATGGAGGA GTATTTCAGA 
GAGAAAGATG ACCGCTTCAA TTCCTCCAAT GACGAAAGGA TACGTGTGAC ATCTGAAAGG 
GTGGAGAACA TGGCGATGCG ATTAGAGGAG GTGAATGAGA GGGAACATTT TATGAAAGCG 
TCCTTGCAGA CTGTAGACAT CCGGCTCGCC CAGATGGAGG AGATGATCGG CCGCATTGCT 
GTGGCCCTAG AGAGAGTTGC TGGAATGGAC AGAGGCGAAG TTAACAAAGC CCGGTCCAGG 
ACATCATCAG ACTGCACTGA TACCAATTAC ATCCTGCGCC AAAGCAGTTT CAACAGCCAA 
GAAGGCAATT CCTACAGGCT ACAGGAGTCT CTGGAGCAGG GGGGCGACGA GTCCATTTCT 
CCTACTTCCC CAACCGCCCT GGCTCCCCGT GTCCGCAGTC ACTCCTTCTA CGTGAGCCAC 
TCTTCCAAAG ACAGGAGTGG GGCGGACCGT GGAGAGGGCT TCTTTAAAGA CAAGTTGTTC 
AGCCTCCACC GGGCCAACAG CTCACAGTCT GTGTCCTCAG GAGCAGGTCC CAAAGAGTCC 
AAGCCAACCC CTTTGAATAC TTTATCCGTG CAGCAGCAGC TCCGCCCTTC TTCCTGCATA 
GACATCTATG TTTCTGCCTC AGAGGACGTC CCGCCCACTG AGAGCTTCTT GGATTCGGTT 
CGGACAGTTC CAACTCTGGC TCGAGACTCA TCTCTTCATT CGGAGATCAT GGAGGCGGTG 
CTCTCAGGGG GACGGGACTG TAGTGGAAGG GCAGGCGGCA GCGAAAGACA GTCGGATGGT 
ACAGTCCTGT TTGAGGACAG CGCCGCCGCT GACCTATCGC TCTGCTCTGC CCACCTGCTG 
CCGGATTCCT TGCCCCCCTG GGACCTGGAC CCCTCACCGC CACCATCAGC TGGTGTACTA 
GAGCGGTCCA AAAGTAGTCG CTTCCTGTCT ACAGCAGGGC CTCTGTTTCT GGACGAGCCT 
CATTTGGTTA AGTCACACAG TCTGATGTTC ACATCACGTG GGTACTATGG TGGCATGGGT 
GTGCAGGTGA AGGCGGCCGA ATACACCAGC ATCACAGATT GCATCGACAC CCGTTGCGTC 
TCAACCCCTT ACCCTGTGCC AGAGCGGTCA GACTCACCTG GAGGATCTTT CACCTTCGAT 
AAGCCTCAAG ACCTTGGCGT CTCGCACCCT GAGAGAGACG CTGAGCTCAG CCACGCTGAG 
TCTGACTTAG AGGAGCCAGC AGAGGGTTCG GGAGACACAT GTAAGGTGGG TCAGAGTAGC 
AGTAGTGGGG TAATAGGTGC AGACCTGGGC CTGGGCCTCG CACTAGGCCC GTTTTGCTCA 
CCCATCTCTA GACTAGAGCG AGCAAACAGC TGCTCCTCTT CAGAGGAGTC GCACTCCAAC 
ATCTACTCTC GAAAGAGCTT CTCAATAAGC GAGAGAATGG ACAAGGGCCG GGGGAGCTCC 
AGGAACCCTT TCCAGAAGGC CAGAACCGGG GCGAGGCTGG AGGGTAAGAC AGACTCACTG 
TCTATGAGGA AAATGGCCAA ACCGTCAGCT TTCAGAAGTT TTGACAGCAG ACACAACTAC 
ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm3 ( XM_005171931.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171988.1 Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X7, mRNA.

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CloneID	ODa10547
Clone ID Related Accession (Same CDS sequence)	XM_005171931.4 , XM_005171931.3
Accession Version	XM_005171931.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transient receptor potential cation channel subfamily M member 3 isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005171931.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGCC CTTTTCCGGA TGGCGGGAAC GTCCGGCCGG GAGCGATGAA CCAGCTGGAT 
GCTCCTCGGC CACTCAACTG GACCATACGG AAACTGTGCC ATGCAGCCTT CCTCCCGTCT 
GTGCGCCTGC TTAAGGCACA GAAATCATGG ATAGAAAGAG CGTTCAGCAA GAGAGAATGT 
GTGCACATTA TTGTAAGCAC CAAAGACCAC CATAGGTGCT GTTGTGGCCG TCTAATTGGT 
CAACATGTTG GCCTGCCCCC CAGCATCTCA TCCAATCAGA ATGAGAAGTC AGAACGCGTG 
CCAAAGAATG ACAGCTTGTC AGAGAAGTGG TCCATCAGCA AGCACACCCA GCTCAGTCCC 
ACCGACGCTT TTGGGACCAT CGAGTTCCAG GGAGGAGGAC ACTCTAATAA AGCAATGTAT 
GTACGAGTGT CTTACGACAC AAAACCAGAT TTACTGCTGC ATCTGATGAC AAAGGAGTGG 
CAACTGGAGC TTCCCAAGCT GCTCATCTCT GTTCACGGAG GACTTCAAAA CTTTGAGTTG 
CAGCCCAAAC TAAAGCAAGT CTTCGGCAAA GGTCTTATAA AGGCTGCAAT GACTACCGGA 
GCCTGGATCT TCACTGGAGG AGTTAATACT GGAGTCATCA GACATGTTGG TGATGCTCTG 
AAAGATCATG CTTCCAAATC AAGAGGGAAG ATCTGCACTA TCGGCATTGC CCCCTGGGGG 
ATTGTGGAGA ACCAGGAAGA CCTGGTGGGC AAAGATGTGG TCCGGCCATA CCAGACGATG 
TCTAACCCTC TTAGCAAGCT AACAGTACTC AACAGCCTAC ACTCACACTT CATTTTGGCT 
GATAATGGCA CCACCGGAAA GTACGGAGCA GAAGTCAAAC TGCGCAGGCA GCTGGAGAAG 
CACATCTCTC TGCAAAAGAT TAACACACGG ATTGGACAGG GTGTTCCAGT AGTGGCTCTG 
ATAGTGGAGG GGGGTCCGAA TGTCATCTCT ATCGTCCTGG AGTACCTAAG AGACACCCCT 
CCGGTGCCTG TTGTGGTGTG TGACGGCAGC GGTCGAGCCT CTGACATCCT GGCCTTTGGG 
CACAAGTACT CAGAGGAGGG CGGAATCATA AACGAATCTT TGAGAGACCA GTTATTAGTC 
ACCATTCAGA AGACGTTCAC ATACAGCCGG ACGCAGGCCC AGCATCTCTT CATCATTCTG 
ATGGAGTGCA TGAAGAAGAA AGAACTGATC ACAGTGTTTC GGATGGGATC AGAGGGACAC 
CAGGACATCG ATCTAGCCAT TCTAACAGCT CTTCTGAAAG GAGCGAATGC ATCAGCTCCA 
GATCAGCTAA GTCTGGCTTT GGCCTGGAAC AGAGTTGACA TCGCCCGGAG CCAGATCTTC 
ATTTACGGAC AGCAGTGGCC TGTTGGCTCT CTGGAGCAGT CTATGCTGGA CGCGCTGGTG 
CTGGACCGAG TGGACTTTGT AAAGCTGTTG ATTGAGAACG GAGTGAGCAT GCACCGTTTC 
CTGACACTCT CCAGACTTGA AGAGCTTTAT AATACGAGGC ACGGACCATC CAACACCTTG 
TACCACCTTG TCAGAGATGT GAAAAAGCAA GAATATCCAG GGTTCAGTTG GATCTACCTC 
AAGGGTAACC TGCCCCCCGA CTACCGCATT AGCCTGATTG ACATCGGATT GGTTATTGAA 
TACCTCATGG GTGGAGCCTA TCGCTGCAAT TACACCAGGA AGAGATTTCG GACCCTCTAT 
CACAACCTCT TTGGACCCAA AAGACCAAAA GCCCTGAAGC TGCTTGGAAT GGAGGATGAC 
ATGCCCATTA GACGAGGGCG TCAGAAGACT ACGCGGAAGC GAGAGGAGGA AGTAGATATT 
GACCTGGATG ACCCAGAAAT CAATCATTTC CCTTTCCCAT TCCATGAGCT GATGGTTTGG 
GCTGTGCTGA TGAAACGGCA GAAGATGGCA CTGTTCTTCT GGCAGCATGG AGAGGAGGCC 
ATGGCTAAAG CGCTGGTGGC CTGCAAGCTC TGTAAGGCAA TGGCTCACGA AGCTTCTGAG 
AACGACATGG TCGATGACAT TTCACAGGAG CTCAATCAAA ACTCAAGGGA GTTTGGTCAG 
CTGGCTGTTG AGCTGCTCGA TCAGTCCTAT AAGCAGGATG AACAGATGGC TATGAAGCTG 
TTGACCTACG AGCTGAAGAA CTGGAGTAAT GCCACCTGTC TGCAGCTGGC AGTGGCTGCC 
AAGCACCGAG ACTTCATCGC CCACACCTGT AGCCAGATGC TGCTCACAGA CATGTGGATG 
GGACGTCTGC GCATGCGCAA GAACTCTGGC CTGAAGGTTA TCTTGGGCCT GCTTCTCCCC 
CCTTCCATAT TGAGTCTGGA GTTTAAAAAT AAAGACGAGA TGTCATACAT GCCTCAGGAT 
CAGGACACAT ATCTGCAGGA GAAGGATGTG GATGAGCCCG AGAAACAAGC TAAGGAGAAA 
GAGGAGGAGG ACATGGAGTT CACAGCTATG TTGGGAAACG TCAGTTCAGA AGCATCGCGC 
AAGAAACAGG TGGAGGAGGT TCAGAACCGG CATCGGCTCA TTCCATTGGG TCGCAAAATA 
TATGAGTTTT ACAACGCCCC TATCGTCAAG TTCTGGTTTC ACACGCTGGC GTATGTAGGA 
TACTTGATGC TCTTCAACTA TATTGTGCTG GTGAAGATGG ACCTGTGGCC TTCTCCTCAG 
GAGTGGATTG TTATTGCCTA CATATTCACC AATGGCATCG AGAAGATGAG AGAGATCCTG 
ATGTCCGAAC CTGGGAAGCT GCTTCAGAAG GTGAAAGTGT GGTTACAGGA GTACTGGAAC 
ATCACTGACC TCATGGCCAT CCTCATCTTC TCCATCGGGA TGGTGCTTCG TCTGCAGGAC 
CCGCCGCTGA TGAGCTACGG CAGGGTCATT TACTGCGTCA ATATCATTTA TTGGTACATA 
CGACTCCTCG ACATCTTCGG AGTGAACAAA TACCTGGGTC CATACGTCAT GATGATCGGC 
AAGATGATGA TTGACATGAT GTATTTTGTG ATCATCATGT TGGTGGTGCT GATGAGTTTC 
GGTGTGGCGC GTCAGGCCAT CCTTAATCCC AATGAAGACC CATCCTGGAT GCTGGCACGC 
AATATCTTCT TCATGCCATA CTGGATGATC TACGGAGAGG TGTTTGCTGA CCAAATCGAC 
CCTCCTTGTG GTCAGAACAT CACCACAGAA GAAGGAGTTG TCATGCCTCT TCCACCCTGC 
AAAACTGGAG CTTGGATTGT CCCTGCCATA ATGGCCTGCT ACCTGCTGGT GGCCAACATC 
CTGCTAGTTA ACTTGCTCAT TGCTGTCTTT AATAACACTT TCTTTGAAGT CAAGTCCATT 
TCCAACCAGG TGTGGAAGTT TCAGAGGTAT CAGCTGATCA TGACCTTCCA TGAACGCCCG 
GTCCTTCCTC CTCCACTCAT CATCTTCAGC CACATAACTA TGGTTCTCAA ACACCTGTGC 
TGTCGCTGGA GGAAGCACGA TGAGGATGAA CGGGACTATG GGCTGAAGCT TTTCATCACT 
GAAGATGAAC TGAAGAAAGT GCATGACTTT GAAGAGCAGT GCATGGAGGA GTATTTCAGA 
GAGAAAGATG ACCGCTTCAA TTCCTCCAAT GACGAAAGGA TACGTGTGAC ATCTGAAAGG 
GTGGAGAACA TGGCGATGCG ATTAGAGGAG GTGAATGAGA GGGAACATTT TATGAAAGCG 
TCCTTGCAGA CTGTAGACAT CCGGCTCGCC CAGATGGAGG AGATGATCGG CCGCATTGCT 
GTGGCCCTAG AGAGAGTTGC TGGAATGGAC AGAGGCGAAG TTAACAAAGC CCGGTCCAGG 
ACATCATCAG ACTGCACTGA TACCAATTAC ATCCTGCGCC AAAGCAGTTT CAACAGCCAA 
GAAGGCAATT CCTACAGGCT ACAGGAGTCT CTGGAGCAGG GGGGCGACGA GTCCATTTCT 
CCTACTTCCC CAACCGCCCT GGCTCCCCGT GTCCGCAGTC ACTCCTTCTA CGTGAGCCAC 
TCTTCCAAAG ACAGGAGTGG GGCGGACCGT GGAGAGGGCT TCTTTAAAGA CAAGTTGTTC 
AGCCTCCACC GGGCCAACAG CTCACAGTCT GTGTCCTCAG GAGCAGGTCC CAAAGAGTCC 
AAGCCAACCC CTTTGAATAC TTTATCCGTG CAGCAGCAGC TCCGCCCTTC TTCCTGCATA 
GACATCTATG TTTCTGCCTC AGAGGACGTC CCGCCCACTG AGAGCTTCTT GGATTCGGTT 
CGGACAGTTC CAACTCTGGC TCGAGACTCA TCTCTTCATT CGGAGATCAT GGAGGCGGTG 
CTCTCAGGGG GACGGGACTG TAGTGGAAGG GCAGGCGGCA GCGAAAGACA GTCGGATGGT 
ACAGTCCTGT TTGAGGACAG CGCCGCCGCT GACCTATCGC TCTGCTCTGC CCACCTGCTG 
CCGGATTCCT TGCCCCCCTG GGACCTGGAC CCCTCACCGC CACCATCAGC TGGTGTACTA 
GAGCGGTCCA AAAGTAGTCG CTTCCTGTCT ACAGCAGGGC CTCTGTTTCT GGACGAGCCT 
CATTTGGTTA AGTCACACAG TCTGATGTTC ACATCACGTG GGTACTATGG TGGCATGGGT 
GTGCAGGTGA AGGCGGCCGA ATACACCAGC ATCACAGATT GCATCGACAC CCGTTGCGTC 
TCAACCCCTT ACCCTGTGCC AGAGCGGTCA GACTCACCTG GAGGATCTTT CACCTTCGAT 
AAGCCTCAAG ACCTTGGCGT CTCGCACCCT GAGAGAGACG CTGAGCTCAG CCACGCTGAG 
TCTGACTTAG AGGAGCCAGC AGAGGGTTCG GGAGACACAT GTAAGGTGGG TCAGAGTAGC 
AGTAGTGGGG TAATAGGTGC AGACCTGGGC CTGGGCCTCG CACTAGGCCC GTTTTGCTCA 
CCCATCTCTA GACTAGAGCG AGCAAACAGC TGCTCCTCTT CAGAGGAGTC GCACTCCAAC 
ATCTACTCTC GAAAGAGCTT CTCAATAAGC GAGAGAATGG ACAAGGGCCG GGGGAGCTCC 
AGGAACCCTT TCCAGAAGGC CAGAACCGGG GCGAGGCTGG AGGGTAAGAC AGACTCACTG 
TCTATGAGGA AAATGGCCAA ACCGTCAGCT TTCAGAAGTT TTGACAGCAG ACACAACTAC 
ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171988.1
CDS	375..5540
Translation	MWGPFPDGGNVRPGAMNQLDAPRPLNWTIRKLCHAAFLPSVRLLKAQKSWIERAFSKRECVHIIVSTKDHHRCCCGRLIGQHVGLPPSISSNQNEKSERVPKNDSLSEKWSISKHTQLSPTDAFGTIEFQGGGHSNKAMYVRVSYDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLVGKDVVRPYQTMSNPLSKLTVLNSLHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGIINESLRDQLLVTIQKTFTYSRTQAQHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQSMLDALVLDRVDFVKLLIENGVSMHRFLTLSRLEELYNTRHGPSNTLYHLVRDVKKQEYPGFSWIYLKGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDMPIRRGRQKTTRKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNQNSREFGQLAVELLDQSYKQDEQMAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGLLLPPSILSLEFKNKDEMSYMPQDQDTYLQEKDVDEPEKQAKEKEEEDMEFTAMLGNVSSEASRKKQVEEVQNRHRLIPLGRKIYEFYNAPIVKFWFHTLAYVGYLMLFNYIVLVKMDLWPSPQEWIVIAYIFTNGIEKMREILMSEPGKLLQKVKVWLQEYWNITDLMAILIFSIGMVLRLQDPPLMSYGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDPPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHITMVLKHLCCRWRKHDEDERDYGLKLFITEDELKKVHDFEEQCMEEYFREKDDRFNSSNDERIRVTSERVENMAMRLEEVNEREHFMKASLQTVDIRLAQMEEMIGRIAVALERVAGMDRGEVNKARSRTSSDCTDTNYILRQSSFNSQEGNSYRLQESLEQGGDESISPTSPTALAPRVRSHSFYVSHSSKDRSGADRGEGFFKDKLFSLHRANSSQSVSSGAGPKESKPTPLNTLSVQQQLRPSSCIDIYVSASEDVPPTESFLDSVRTVPTLARDSSLHSEIMEAVLSGGRDCSGRAGGSERQSDGTVLFEDSAAADLSLCSAHLLPDSLPPWDLDPSPPPSAGVLERSKSSRFLSTAGPLFLDEPHLVKSHSLMFTSRGYYGGMGVQVKAAEYTSITDCIDTRCVSTPYPVPERSDSPGGSFTFDKPQDLGVSHPERDAELSHAESDLEEPAEGSGDTCKVGQSSSSGVIGADLGLGLALGPFCSPISRLERANSCSSSEESHSNIYSRKSFSISERMDKGRGSSRNPFQKARTGARLEGKTDSLSMRKMAKPSAFRSFDSRHNYT

Target ORF information:

RefSeq Version	XM_005171931.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005171931.3

ORF Insert Sequence:

ATGTGGGGCC CTTTTCCGGA TGGCGGGAAC GTCCGGCCGG GAGCGATGAA CCAGCTGGAT 
GCTCCTCGGC CACTCAACTG GACCATACGG AAACTGTGCC ATGCAGCCTT CCTCCCGTCT 
GTGCGCCTGC TTAAGGCACA GAAATCATGG ATAGAAAGAG CGTTCAGCAA GAGAGAATGT 
GTGCACATTA TTGTAAGCAC CAAAGACCAC CATAGGTGCT GTTGTGGCCG TCTAATTGGT 
CAACATGTTG GCCTGCCCCC CAGCATCTCA TCCAATCAGA ATGAGAAGTC AGAACGCGTG 
CCAAAGAATG ACAGCTTGTC AGAGAAGTGG TCCATCAGCA AGCACACCCA GCTCAGTCCC 
ACCGACGCTT TTGGGACCAT CGAGTTCCAG GGAGGAGGAC ACTCTAATAA AGCAATGTAT 
GTACGAGTGT CTTACGACAC AAAACCAGAT TTACTGCTGC ATCTGATGAC AAAGGAGTGG 
CAACTGGAGC TTCCCAAGCT GCTCATCTCT GTTCACGGAG GACTTCAAAA CTTTGAGTTG 
CAGCCCAAAC TAAAGCAAGT CTTCGGCAAA GGTCTTATAA AGGCTGCAAT GACTACCGGA 
GCCTGGATCT TCACTGGAGG AGTTAATACT GGAGTCATCA GACATGTTGG TGATGCTCTG 
AAAGATCATG CTTCCAAATC AAGAGGGAAG ATCTGCACTA TCGGCATTGC CCCCTGGGGG 
ATTGTGGAGA ACCAGGAAGA CCTGGTGGGC AAAGATGTGG TCCGGCCATA CCAGACGATG 
TCTAACCCTC TTAGCAAGCT AACAGTACTC AACAGCCTAC ACTCACACTT CATTTTGGCT 
GATAATGGCA CCACCGGAAA GTACGGAGCA GAAGTCAAAC TGCGCAGGCA GCTGGAGAAG 
CACATCTCTC TGCAAAAGAT TAACACACGG ATTGGACAGG GTGTTCCAGT AGTGGCTCTG 
ATAGTGGAGG GGGGTCCGAA TGTCATCTCT ATCGTCCTGG AGTACCTAAG AGACACCCCT 
CCGGTGCCTG TTGTGGTGTG TGACGGCAGC GGTCGAGCCT CTGACATCCT GGCCTTTGGG 
CACAAGTACT CAGAGGAGGG CGGAATCATA AACGAATCTT TGAGAGACCA GTTATTAGTC 
ACCATTCAGA AGACGTTCAC ATACAGCCGG ACGCAGGCCC AGCATCTCTT CATCATTCTG 
ATGGAGTGCA TGAAGAAGAA AGAACTGATC ACAGTGTTTC GGATGGGATC AGAGGGACAC 
CAGGACATCG ATCTAGCCAT TCTAACAGCT CTTCTGAAAG GAGCGAATGC ATCAGCTCCA 
GATCAGCTAA GTCTGGCTTT GGCCTGGAAC AGAGTTGACA TCGCCCGGAG CCAGATCTTC 
ATTTACGGAC AGCAGTGGCC TGTTGGCTCT CTGGAGCAGT CTATGCTGGA CGCGCTGGTG 
CTGGACCGAG TGGACTTTGT AAAGCTGTTG ATTGAGAACG GAGTGAGCAT GCACCGTTTC 
CTGACACTCT CCAGACTTGA AGAGCTTTAT AATACGAGGC ACGGACCATC CAACACCTTG 
TACCACCTTG TCAGAGATGT GAAAAAGCAA GAATATCCAG GGTTCAGTTG GATCTACCTC 
AAGGGTAACC TGCCCCCCGA CTACCGCATT AGCCTGATTG ACATCGGATT GGTTATTGAA 
TACCTCATGG GTGGAGCCTA TCGCTGCAAT TACACCAGGA AGAGATTTCG GACCCTCTAT 
CACAACCTCT TTGGACCCAA AAGACCAAAA GCCCTGAAGC TGCTTGGAAT GGAGGATGAC 
ATGCCCATTA GACGAGGGCG TCAGAAGACT ACGCGGAAGC GAGAGGAGGA AGTAGATATT 
GACCTGGATG ACCCAGAAAT CAATCATTTC CCTTTCCCAT TCCATGAGCT GATGGTTTGG 
GCTGTGCTGA TGAAACGGCA GAAGATGGCA CTGTTCTTCT GGCAGCATGG AGAGGAGGCC 
ATGGCTAAAG CGCTGGTGGC CTGCAAGCTC TGTAAGGCAA TGGCTCACGA AGCTTCTGAG 
AACGACATGG TCGATGACAT TTCACAGGAG CTCAATCAAA ACTCAAGGGA GTTTGGTCAG 
CTGGCTGTTG AGCTGCTCGA TCAGTCCTAT AAGCAGGATG AACAGATGGC TATGAAGCTG 
TTGACCTACG AGCTGAAGAA CTGGAGTAAT GCCACCTGTC TGCAGCTGGC AGTGGCTGCC 
AAGCACCGAG ACTTCATCGC CCACACCTGT AGCCAGATGC TGCTCACAGA CATGTGGATG 
GGACGTCTGC GCATGCGCAA GAACTCTGGC CTGAAGGTTA TCTTGGGCCT GCTTCTCCCC 
CCTTCCATAT TGAGTCTGGA GTTTAAAAAT AAAGACGAGA TGTCATACAT GCCTCAGGAT 
CAGGACACAT ATCTGCAGGA GAAGGATGTG GATGAGCCCG AGAAACAAGC TAAGGAGAAA 
GAGGAGGAGG ACATGGAGTT CACAGCTATG TTGGGAAACG TCAGTTCAGA AGCATCGCGC 
AAGAAACAGG TGGAGGAGGT TCAGAACCGG CATCGGCTCA TTCCATTGGG TCGCAAAATA 
TATGAGTTTT ACAACGCCCC TATCGTCAAG TTCTGGTTTC ACACGCTGGC GTATGTAGGA 
TACTTGATGC TCTTCAACTA TATTGTGCTG GTGAAGATGG ACCTGTGGCC TTCTCCTCAG 
GAGTGGATTG TTATTGCCTA CATATTCACC AATGGCATCG AGAAGATGAG AGAGATCCTG 
ATGTCCGAAC CTGGGAAGCT GCTTCAGAAG GTGAAAGTGT GGTTACAGGA GTACTGGAAC 
ATCACTGACC TCATGGCCAT CCTCATCTTC TCCATCGGGA TGGTGCTTCG TCTGCAGGAC 
CCGCCGCTGA TGAGCTACGG CAGGGTCATT TACTGCGTCA ATATCATTTA TTGGTACATA 
CGACTCCTCG ACATCTTCGG AGTGAACAAA TACCTGGGTC CATACGTCAT GATGATCGGC 
AAGATGATGA TTGACATGAT GTATTTTGTG ATCATCATGT TGGTGGTGCT GATGAGTTTC 
GGTGTGGCGC GTCAGGCCAT CCTTAATCCC AATGAAGACC CATCCTGGAT GCTGGCACGC 
AATATCTTCT TCATGCCATA CTGGATGATC TACGGAGAGG TGTTTGCTGA CCAAATCGAC 
CCTCCTTGTG GTCAGAACAT CACCACAGAA GAAGGAGTTG TCATGCCTCT TCCACCCTGC 
AAAACTGGAG CTTGGATTGT CCCTGCCATA ATGGCCTGCT ACCTGCTGGT GGCCAACATC 
CTGCTAGTTA ACTTGCTCAT TGCTGTCTTT AATAACACTT TCTTTGAAGT CAAGTCCATT 
TCCAACCAGG TGTGGAAGTT TCAGAGGTAT CAGCTGATCA TGACCTTCCA TGAACGCCCG 
GTCCTTCCTC CTCCACTCAT CATCTTCAGC CACATAACTA TGGTTCTCAA ACACCTGTGC 
TGTCGCTGGA GGAAGCACGA TGAGGATGAA CGGGACTATG GGCTGAAGCT TTTCATCACT 
GAAGATGAAC TGAAGAAAGT GCATGACTTT GAAGAGCAGT GCATGGAGGA GTATTTCAGA 
GAGAAAGATG ACCGCTTCAA TTCCTCCAAT GACGAAAGGA TACGTGTGAC ATCTGAAAGG 
GTGGAGAACA TGGCGATGCG ATTAGAGGAG GTGAATGAGA GGGAACATTT TATGAAAGCG 
TCCTTGCAGA CTGTAGACAT CCGGCTCGCC CAGATGGAGG AGATGATCGG CCGCATTGCT 
GTGGCCCTAG AGAGAGTTGC TGGAATGGAC AGAGGCGAAG TTAACAAAGC CCGGTCCAGG 
ACATCATCAG ACTGCACTGA TACCAATTAC ATCCTGCGCC AAAGCAGTTT CAACAGCCAA 
GAAGGCAATT CCTACAGGCT ACAGGAGTCT CTGGAGCAGG GGGGCGACGA GTCCATTTCT 
CCTACTTCCC CAACCGCCCT GGCTCCCCGT GTCCGCAGTC ACTCCTTCTA CGTGAGCCAC 
TCTTCCAAAG ACAGGAGTGG GGCGGACCGT GGAGAGGGCT TCTTTAAAGA CAAGTTGTTC 
AGCCTCCACC GGGCCAACAG CTCACAGTCT GTGTCCTCAG GAGCAGGTCC CAAAGAGTCC 
AAGCCAACCC CTTTGAATAC TTTATCCGTG CAGCAGCAGC TCCGCCCTTC TTCCTGCATA 
GACATCTATG TTTCTGCCTC AGAGGACGTC CCGCCCACTG AGAGCTTCTT GGATTCGGTT 
CGGACAGTTC CAACTCTGGC TCGAGACTCA TCTCTTCATT CGGAGATCAT GGAGGCGGTG 
CTCTCAGGGG GACGGGACTG TAGTGGAAGG GCAGGCGGCA GCGAAAGACA GTCGGATGGT 
ACAGTCCTGT TTGAGGACAG CGCCGCCGCT GACCTATCGC TCTGCTCTGC CCACCTGCTG 
CCGGATTCCT TGCCCCCCTG GGACCTGGAC CCCTCACCGC CACCATCAGC TGGTGTACTA 
GAGCGGTCCA AAAGTAGTCG CTTCCTGTCT ACAGCAGGGC CTCTGTTTCT GGACGAGCCT 
CATTTGGTTA AGTCACACAG TCTGATGTTC ACATCACGTG GGTACTATGG TGGCATGGGT 
GTGCAGGTGA AGGCGGCCGA ATACACCAGC ATCACAGATT GCATCGACAC CCGTTGCGTC 
TCAACCCCTT ACCCTGTGCC AGAGCGGTCA GACTCACCTG GAGGATCTTT CACCTTCGAT 
AAGCCTCAAG ACCTTGGCGT CTCGCACCCT GAGAGAGACG CTGAGCTCAG CCACGCTGAG 
TCTGACTTAG AGGAGCCAGC AGAGGGTTCG GGAGACACAT GTAAGGTGGG TCAGAGTAGC 
AGTAGTGGGG TAATAGGTGC AGACCTGGGC CTGGGCCTCG CACTAGGCCC GTTTTGCTCA 
CCCATCTCTA GACTAGAGCG AGCAAACAGC TGCTCCTCTT CAGAGGAGTC GCACTCCAAC 
ATCTACTCTC GAAAGAGCTT CTCAATAAGC GAGAGAATGG ACAAGGGCCG GGGGAGCTCC 
AGGAACCCTT TCCAGAAGGC CAGAACCGGG GCGAGGCTGG AGGGTAAGAC AGACTCACTG 
TCTATGAGGA AAATGGCCAA ACCGTCAGCT TTCAGAAGTT TTGACAGCAG ACACAACTAC 
ACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm3 ( XM_009301557.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299832.1 Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X8, mRNA.

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CloneID	ODa44718
Clone ID Related Accession (Same CDS sequence)	XM_009301557.3 , XM_009301557.2
Accession Version	XM_009301557.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily M member 3 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301557.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAAAA GAAAATGGGA GGGGAGGAGA GAAGGCTTCA AGGTGAGCTC CACGAGGGAG 
GAGGCACAGG GGCCTGGACG CCCCTCCACC AGAGGGCGCT TCTCCGAATC TTGGAAGAGA 
CTGAGCTCCA GACAGGGGTC TACCAAGAGG TCAGGACTGG CCTCCCAGCA AGCTGCGGCA 
CAGAAATCAT GGATAGAAAG AGCGTTCAGC AAGAGAGAAT GTGTGCACAT TATTGTAAGC 
ACCAAAGACC ACCATAGGTG CTGTTGTGGC CGTCTAATTG GTCAACATGT TGGCCTGCCC 
CCCAGCATCT CATCCAATCA GAATGAGAAG TCAGAACGCG TGCCAAAGAA TGACAGCTTG 
TCAGAGAAGT GGTCCATCAG CAAGCACACC CAGCTCAGTC CCACCGACGC TTTTGGGACC 
ATCGAGTTCC AGGGAGGAGG ACACTCTAAT AAAGCAATGT ATGTACGAGT GTCTTACGAC 
ACAAAACCAG ATTTACTGCT GCATCTGATG ACAAAGGAGT GGCAACTGGA GCTTCCCAAG 
CTGCTCATCT CTGTTCACGG AGGACTTCAA AACTTTGAGT TGCAGCCCAA ACTAAAGCAA 
GTCTTCGGCA AAGGTCTTAT AAAGGCTGCA ATGACTACCG GAGCCTGGAT CTTCACTGGA 
GGAGTTAATA CTGGAGTCAT CAGACATGTT GGTGATGCTC TGAAAGATCA TGCTTCCAAA 
TCAAGAGGGA AGATCTGCAC TATCGGCATT GCCCCCTGGG GGATTGTGGA GAACCAGGAA 
GACCTGGTGG GCAAAGATGT GGTCCGGCCA TACCAGACGA TGTCTAACCC TCTTAGCAAG 
CTAACAGTAC TCAACAGCCT ACACTCACAC TTCATTTTGG CTGATAATGG CACCACCGGA 
AAGTACGGAG CAGAAGTCAA ACTGCGCAGG CAGCTGGAGA AGCACATCTC TCTGCAAAAG 
ATTAACACAC GGATTGGACA GGGTGTTCCA GTAGTGGCTC TGATAGTGGA GGGGGGTCCG 
AATGTCATCT CTATCGTCCT GGAGTACCTA AGAGACACCC CTCCGGTGCC TGTTGTGGTG 
TGTGACGGCA GCGGTCGAGC CTCTGACATC CTGGCCTTTG GGCACAAGTA CTCAGAGGAG 
GGCGGAATCA TAAACGAATC TTTGAGAGAC CAGTTATTAG TCACCATTCA GAAGACGTTC 
ACATACAGCC GGACGCAGGC CCAGCATCTC TTCATCATTC TGATGGAGTG CATGAAGAAG 
AAAGAACTGA TCACAGTGTT TCGGATGGGA TCAGAGGGAC ACCAGGACAT CGATCTAGCC 
ATTCTAACAG CTCTTCTGAA AGGAGCGAAT GCATCAGCTC CAGATCAGCT AAGTCTGGCT 
TTGGCCTGGA ACAGAGTTGA CATCGCCCGG AGCCAGATCT TCATTTACGG ACAGCAGTGG 
CCTGTTGGCT CTCTGGAGCA GTCTATGCTG GACGCGCTGG TGCTGGACCG AGTGGACTTT 
GTAAAGCTGT TGATTGAGAA CGGAGTGAGC ATGCACCGTT TCCTGACACT CTCCAGACTT 
GAAGAGCTTT ATAATACGAG GCACGGACCA TCCAACACCT TGTACCACCT TGTCAGAGAT 
GTGAAAAAGC AAGAATATCC AGGGTTCAGT TGGATCTACC TCAAGGGTAA CCTGCCCCCC 
GACTACCGCA TTAGCCTGAT TGACATCGGA TTGGTTATTG AATACCTCAT GGGTGGAGCC 
TATCGCTGCA ATTACACCAG GAAGAGATTT CGGACCCTCT ATCACAACCT CTTTGGACCC 
AAAAGACCAA AAGCCCTGAA GCTGCTTGGA ATGGAGGATG ACATGCCCAT TAGACGAGGG 
CGTCAGAAGA CTACGCGGAA GCGAGAGGAG GAAGTAGATA TTGACCTGGA TGACCCAGAA 
ATCAATCATT TCCCTTTCCC ATTCCATGAG CTGATGGTTT GGGCTGTGCT GATGAAACGG 
CAGAAGATGG CACTGTTCTT CTGGCAGCAT GGAGAGGAGG CCATGGCTAA AGCGCTGGTG 
GCCTGCAAGC TCTGTAAGGC AATGGCTCAC GAAGCTTCTG AGAACGACAT GGTCGATGAC 
ATTTCACAGG AGCTCAATCA AAACTCAAGG GAGTTTGGTC AGCTGGCTGT TGAGCTGCTC 
GATCAGTCCT ATAAGCAGGA TGAACAGATG GCTATGAAGC TGTTGACCTA CGAGCTGAAG 
AACTGGAGTA ATGCCACCTG TCTGCAGCTG GCAGTGGCTG CCAAGCACCG AGACTTCATC 
GCCCACACCT GTAGCCAGAT GCTGCTCACA GACATGTGGA TGGGACGTCT GCGCATGCGC 
AAGAACTCTG GCCTGAAGGT TATCTTGGGC CTGCTTCTCC CCCCTTCCAT ATTGAGTCTG 
GAGTTTAAAA ATAAAGACGA GATGTCATAC ATGCCTCAGG ATCAGGACAC ATATCTGCAG 
GAGAAGGATG TGGATGAGCC CGAGAAACAA GCTAAGGAGA AAGAGGAGGA GGACATGGAG 
TTCACAGTAA GATCTTACTG TGAGACGCAG TACAACTCCG TGGCTATGTT GGGAAACGTC 
AGTTCAGAAG CATCGCGCAA GAAACAGGTG GAGGAGGTTC AGAACCGGCA TCGGCTCATT 
CCATTGGGTC GCAAAATATA TGAGTTTTAC AACGCCCCTA TCGTCAAGTT CTGGTTTCAC 
ACGCTGGCGT ATGTAGGATA CTTGATGCTC TTCAACTATA TTGTGCTGGT GAAGATGGAC 
CTGTGGCCTT CTCCTCAGGA GTGGATTGTT ATTGCCTACA TATTCACCAA TGGCATCGAG 
AAGATGAGAG AGATCCTGAT GTCCGAACCT GGGAAGCTGC TTCAGAAGGT GAAAGTGTGG 
TTACAGGAGT ACTGGAACAT CACTGACCTC ATGGCCATCC TCATCTTCTC CATCGGGATG 
GTGCTTCGTC TGCAGGACCC GCCGCTGATG AGCTACGGCA GGGTCATTTA CTGCGTCAAT 
ATCATTTATT GGTACATACG ACTCCTCGAC ATCTTCGGAG TGAACAAATA CCTGGGTCCA 
TACGTCATGA TGATCGGCAA GATGATGATT GACATGATGT ATTTTGTGAT CATCATGTTG 
GTGGTGCTGA TGAGTTTCGG TGTGGCGCGT CAGGCCATCC TTAATCCCAA TGAAGACCCA 
TCCTGGATGC TGGCACGCAA TATCTTCTTC ATGCCATACT GGATGATCTA CGGAGAGGTG 
TTTGCTGACC AAATCGACCC TCCTTGTGGT CAGAACATCA CCACAGAAGA AGGAGTTGTC 
ATGCCTCTTC CACCCTGCAA AACTGGAGCT TGGATTGTCC CTGCCATAAT GGCCTGCTAC 
CTGCTGGTGG CCAACATCCT GCTAGTTAAC TTGCTCATTG CTGTCTTTAA TAACACTTTC 
TTTGAAGTCA AGTCCATTTC CAACCAGGTG TGGAAGTTTC AGAGGTATCA GCTGATCATG 
ACCTTCCATG AACGCCCGGT CCTTCCTCCT CCACTCATCA TCTTCAGCCA CATAACTATG 
GTTCTCAAAC ACCTGTGCTG TCGCTGGAGG AAGCACGATG AGGATGAACG GGACTATGGG 
CTGAAGCTTT TCATCACTGA AGATGAACTG AAGAAAGTGC ATGACTTTGA AGAGCAGTGC 
ATGGAGGAGT ATTTCAGAGA GAAAGATGAC CGCTTCAATT CCTCCAATGA CGAAAGGATA 
CGTGTGACAT CTGAAAGGGT GGAGAACATG GCGATGCGAT TAGAGGAGGT GAATGAGAGG 
GAACATTTTA TGAAAGCGTC CTTGCAGACT GTAGACATCC GGCTCGCCCA GATGGAGGAG 
ATGATCGGCC GCATTGCTGT GGCCCTAGAG AGAGTTGCTG GAATGGACAG AGGCGAAGTT 
AACAAAGCCC GGTCCAGGAC ATCATCAGAC TGCACTGATA CCAATTACAT CCTGCGCCAA 
AGCAGTTTCA ACAGCCAAGA AGGCAATTCC TACAGGCTAC AGGAGTCTCT GGAGCAGGGG 
GGCGACGAGT CCATTTCTCC TACTTCCCCA ACCGCCCTGG CTCCCCGTGT CCGCAGTCAC 
TCCTTCTACG TGAGCCACTC TTCCAAAGAC AGGAGTGGGG CGGACCGTGG AGAGGGCTTC 
TTTAAAGACA AGTTGTTCAG CCTCCACCGG GCCAACAGCT CACAGTCTGT GTCCTCAGGA 
GCAGGTCCCA AAGAGTCCAA GCCAACCCCT TTGAATACTT TATCCGTGCA GCAGCAGCTC 
CGCCCTTCTT CCTGCATAGA CATCTATGTT TCTGCCTCAG AGGACGTCCC GCCCACTGAG 
AGCTTCTTGG ATTCGGTTCG GACAGTTCCA ACTCTGGCTC GAGACTCATC TCTTCATTCG 
GAGATCATGG AGGCGGTGCT CTCAGGGGGA CGGGACTGTA GTGGAAGGGC AGGCGGCAGC 
GAAAGACAGT CGGATGGTAC AGTCCTGTTT GAGGACAGCG CCGCCGCTGA CCTATCGCTC 
TGCTCTGCCC ACCTGCTGCC GGATTCCTTG CCCCCCTGGG ACCTGGACCC CTCACCGCCA 
CCATCAGCTG GTGTACTAGA GCGGTCCAAA AGTAGTCGCT TCCTGTCTAC AGCAGGGCCT 
CTGTTTCTGG ACGAGCCTCA TTTGGTTAAG TCACACAGTC TGATGTTCAC ATCACGTGGG 
TACTATGGTG GCATGGGTGT GCAGGTGAAG GCGGCCGAAT ACACCAGCAT CACAGATTGC 
ATCGACACCC GTTGCGTCTC AACCCCTTAC CCTGTGCCAG AGCGGTCAGA CTCACCTGGA 
GGATCTTTCA CCTTCGATAA GCCTCAAGAC CTTGGCGTCT CGCACCCTGA GAGAGACGCT 
GAGCTCAGCC ACGCTGAGTC TGACTTAGAG GAGCCAGCAG AGGGTTCGGG AGACACATGT 
AAGGTGGGTC AGAGTAGCAG TAGTGGGGTA ATAGGTGCAG ACCTGGGCCT GGGCCTCGCA 
CTAGGCCCGT TTTGCTCACC CATCTCTAGA CTAGAGCGAG CAAACAGCTG CTCCTCTTCA 
GAGGAGTCGC ACTCCAACAT CTACTCTCGA AAGAGCTTCT CAATAAGCGA GAGAATGGAC 
AAGGGCCGGG GGAGCTCCAG GAACCCTTTC CAGAAGGCCA GAACCGGGGC GAGGCTGGAG 
GGTAAGACAG ACTCACTGTC TATGAGGAAA ATGGCCAAAC CGTCAGCTTT CAGAAGTTTT 
GACAGCAGAC ACAACTACAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299832.1
CDS	378..5621
Translation	MTKRKWEGRREGFKVSSTREEAQGPGRPSTRGRFSESWKRLSSRQGSTKRSGLASQQAAAQKSWIERAFSKRECVHIIVSTKDHHRCCCGRLIGQHVGLPPSISSNQNEKSERVPKNDSLSEKWSISKHTQLSPTDAFGTIEFQGGGHSNKAMYVRVSYDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLVGKDVVRPYQTMSNPLSKLTVLNSLHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGIINESLRDQLLVTIQKTFTYSRTQAQHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQSMLDALVLDRVDFVKLLIENGVSMHRFLTLSRLEELYNTRHGPSNTLYHLVRDVKKQEYPGFSWIYLKGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDMPIRRGRQKTTRKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNQNSREFGQLAVELLDQSYKQDEQMAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGLLLPPSILSLEFKNKDEMSYMPQDQDTYLQEKDVDEPEKQAKEKEEEDMEFTVRSYCETQYNSVAMLGNVSSEASRKKQVEEVQNRHRLIPLGRKIYEFYNAPIVKFWFHTLAYVGYLMLFNYIVLVKMDLWPSPQEWIVIAYIFTNGIEKMREILMSEPGKLLQKVKVWLQEYWNITDLMAILIFSIGMVLRLQDPPLMSYGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDPPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHITMVLKHLCCRWRKHDEDERDYGLKLFITEDELKKVHDFEEQCMEEYFREKDDRFNSSNDERIRVTSERVENMAMRLEEVNEREHFMKASLQTVDIRLAQMEEMIGRIAVALERVAGMDRGEVNKARSRTSSDCTDTNYILRQSSFNSQEGNSYRLQESLEQGGDESISPTSPTALAPRVRSHSFYVSHSSKDRSGADRGEGFFKDKLFSLHRANSSQSVSSGAGPKESKPTPLNTLSVQQQLRPSSCIDIYVSASEDVPPTESFLDSVRTVPTLARDSSLHSEIMEAVLSGGRDCSGRAGGSERQSDGTVLFEDSAAADLSLCSAHLLPDSLPPWDLDPSPPPSAGVLERSKSSRFLSTAGPLFLDEPHLVKSHSLMFTSRGYYGGMGVQVKAAEYTSITDCIDTRCVSTPYPVPERSDSPGGSFTFDKPQDLGVSHPERDAELSHAESDLEEPAEGSGDTCKVGQSSSSGVIGADLGLGLALGPFCSPISRLERANSCSSSEESHSNIYSRKSFSISERMDKGRGSSRNPFQKARTGARLEGKTDSLSMRKMAKPSAFRSFDSRHNYT

Target ORF information:

RefSeq Version	XM_009301557.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301557.3

ORF Insert Sequence:

ATGACAAAAA GAAAATGGGA GGGGAGGAGA GAAGGCTTCA AGGTGAGCTC CACGAGGGAG 
GAGGCACAGG GGCCTGGACG CCCCTCCACC AGAGGGCGCT TCTCCGAATC TTGGAAGAGA 
CTGAGCTCCA GACAGGGGTC TACCAAGAGG TCAGGACTGG CCTCCCAGCA AGCTGCGGCA 
CAGAAATCAT GGATAGAAAG AGCGTTCAGC AAGAGAGAAT GTGTGCACAT TATTGTAAGC 
ACCAAAGACC ACCATAGGTG CTGTTGTGGC CGTCTAATTG GTCAACATGT TGGCCTGCCC 
CCCAGCATCT CATCCAATCA GAATGAGAAG TCAGAACGCG TGCCAAAGAA TGACAGCTTG 
TCAGAGAAGT GGTCCATCAG CAAGCACACC CAGCTCAGTC CCACCGACGC TTTTGGGACC 
ATCGAGTTCC AGGGAGGAGG ACACTCTAAT AAAGCAATGT ATGTACGAGT GTCTTACGAC 
ACAAAACCAG ATTTACTGCT GCATCTGATG ACAAAGGAGT GGCAACTGGA GCTTCCCAAG 
CTGCTCATCT CTGTTCACGG AGGACTTCAA AACTTTGAGT TGCAGCCCAA ACTAAAGCAA 
GTCTTCGGCA AAGGTCTTAT AAAGGCTGCA ATGACTACCG GAGCCTGGAT CTTCACTGGA 
GGAGTTAATA CTGGAGTCAT CAGACATGTT GGTGATGCTC TGAAAGATCA TGCTTCCAAA 
TCAAGAGGGA AGATCTGCAC TATCGGCATT GCCCCCTGGG GGATTGTGGA GAACCAGGAA 
GACCTGGTGG GCAAAGATGT GGTCCGGCCA TACCAGACGA TGTCTAACCC TCTTAGCAAG 
CTAACAGTAC TCAACAGCCT ACACTCACAC TTCATTTTGG CTGATAATGG CACCACCGGA 
AAGTACGGAG CAGAAGTCAA ACTGCGCAGG CAGCTGGAGA AGCACATCTC TCTGCAAAAG 
ATTAACACAC GGATTGGACA GGGTGTTCCA GTAGTGGCTC TGATAGTGGA GGGGGGTCCG 
AATGTCATCT CTATCGTCCT GGAGTACCTA AGAGACACCC CTCCGGTGCC TGTTGTGGTG 
TGTGACGGCA GCGGTCGAGC CTCTGACATC CTGGCCTTTG GGCACAAGTA CTCAGAGGAG 
GGCGGAATCA TAAACGAATC TTTGAGAGAC CAGTTATTAG TCACCATTCA GAAGACGTTC 
ACATACAGCC GGACGCAGGC CCAGCATCTC TTCATCATTC TGATGGAGTG CATGAAGAAG 
AAAGAACTGA TCACAGTGTT TCGGATGGGA TCAGAGGGAC ACCAGGACAT CGATCTAGCC 
ATTCTAACAG CTCTTCTGAA AGGAGCGAAT GCATCAGCTC CAGATCAGCT AAGTCTGGCT 
TTGGCCTGGA ACAGAGTTGA CATCGCCCGG AGCCAGATCT TCATTTACGG ACAGCAGTGG 
CCTGTTGGCT CTCTGGAGCA GTCTATGCTG GACGCGCTGG TGCTGGACCG AGTGGACTTT 
GTAAAGCTGT TGATTGAGAA CGGAGTGAGC ATGCACCGTT TCCTGACACT CTCCAGACTT 
GAAGAGCTTT ATAATACGAG GCACGGACCA TCCAACACCT TGTACCACCT TGTCAGAGAT 
GTGAAAAAGC AAGAATATCC AGGGTTCAGT TGGATCTACC TCAAGGGTAA CCTGCCCCCC 
GACTACCGCA TTAGCCTGAT TGACATCGGA TTGGTTATTG AATACCTCAT GGGTGGAGCC 
TATCGCTGCA ATTACACCAG GAAGAGATTT CGGACCCTCT ATCACAACCT CTTTGGACCC 
AAAAGACCAA AAGCCCTGAA GCTGCTTGGA ATGGAGGATG ACATGCCCAT TAGACGAGGG 
CGTCAGAAGA CTACGCGGAA GCGAGAGGAG GAAGTAGATA TTGACCTGGA TGACCCAGAA 
ATCAATCATT TCCCTTTCCC ATTCCATGAG CTGATGGTTT GGGCTGTGCT GATGAAACGG 
CAGAAGATGG CACTGTTCTT CTGGCAGCAT GGAGAGGAGG CCATGGCTAA AGCGCTGGTG 
GCCTGCAAGC TCTGTAAGGC AATGGCTCAC GAAGCTTCTG AGAACGACAT GGTCGATGAC 
ATTTCACAGG AGCTCAATCA AAACTCAAGG GAGTTTGGTC AGCTGGCTGT TGAGCTGCTC 
GATCAGTCCT ATAAGCAGGA TGAACAGATG GCTATGAAGC TGTTGACCTA CGAGCTGAAG 
AACTGGAGTA ATGCCACCTG TCTGCAGCTG GCAGTGGCTG CCAAGCACCG AGACTTCATC 
GCCCACACCT GTAGCCAGAT GCTGCTCACA GACATGTGGA TGGGACGTCT GCGCATGCGC 
AAGAACTCTG GCCTGAAGGT TATCTTGGGC CTGCTTCTCC CCCCTTCCAT ATTGAGTCTG 
GAGTTTAAAA ATAAAGACGA GATGTCATAC ATGCCTCAGG ATCAGGACAC ATATCTGCAG 
GAGAAGGATG TGGATGAGCC CGAGAAACAA GCTAAGGAGA AAGAGGAGGA GGACATGGAG 
TTCACAGTAA GATCTTACTG TGAGACGCAG TACAACTCCG TGGCTATGTT GGGAAACGTC 
AGTTCAGAAG CATCGCGCAA GAAACAGGTG GAGGAGGTTC AGAACCGGCA TCGGCTCATT 
CCATTGGGTC GCAAAATATA TGAGTTTTAC AACGCCCCTA TCGTCAAGTT CTGGTTTCAC 
ACGCTGGCGT ATGTAGGATA CTTGATGCTC TTCAACTATA TTGTGCTGGT GAAGATGGAC 
CTGTGGCCTT CTCCTCAGGA GTGGATTGTT ATTGCCTACA TATTCACCAA TGGCATCGAG 
AAGATGAGAG AGATCCTGAT GTCCGAACCT GGGAAGCTGC TTCAGAAGGT GAAAGTGTGG 
TTACAGGAGT ACTGGAACAT CACTGACCTC ATGGCCATCC TCATCTTCTC CATCGGGATG 
GTGCTTCGTC TGCAGGACCC GCCGCTGATG AGCTACGGCA GGGTCATTTA CTGCGTCAAT 
ATCATTTATT GGTACATACG ACTCCTCGAC ATCTTCGGAG TGAACAAATA CCTGGGTCCA 
TACGTCATGA TGATCGGCAA GATGATGATT GACATGATGT ATTTTGTGAT CATCATGTTG 
GTGGTGCTGA TGAGTTTCGG TGTGGCGCGT CAGGCCATCC TTAATCCCAA TGAAGACCCA 
TCCTGGATGC TGGCACGCAA TATCTTCTTC ATGCCATACT GGATGATCTA CGGAGAGGTG 
TTTGCTGACC AAATCGACCC TCCTTGTGGT CAGAACATCA CCACAGAAGA AGGAGTTGTC 
ATGCCTCTTC CACCCTGCAA AACTGGAGCT TGGATTGTCC CTGCCATAAT GGCCTGCTAC 
CTGCTGGTGG CCAACATCCT GCTAGTTAAC TTGCTCATTG CTGTCTTTAA TAACACTTTC 
TTTGAAGTCA AGTCCATTTC CAACCAGGTG TGGAAGTTTC AGAGGTATCA GCTGATCATG 
ACCTTCCATG AACGCCCGGT CCTTCCTCCT CCACTCATCA TCTTCAGCCA CATAACTATG 
GTTCTCAAAC ACCTGTGCTG TCGCTGGAGG AAGCACGATG AGGATGAACG GGACTATGGG 
CTGAAGCTTT TCATCACTGA AGATGAACTG AAGAAAGTGC ATGACTTTGA AGAGCAGTGC 
ATGGAGGAGT ATTTCAGAGA GAAAGATGAC CGCTTCAATT CCTCCAATGA CGAAAGGATA 
CGTGTGACAT CTGAAAGGGT GGAGAACATG GCGATGCGAT TAGAGGAGGT GAATGAGAGG 
GAACATTTTA TGAAAGCGTC CTTGCAGACT GTAGACATCC GGCTCGCCCA GATGGAGGAG 
ATGATCGGCC GCATTGCTGT GGCCCTAGAG AGAGTTGCTG GAATGGACAG AGGCGAAGTT 
AACAAAGCCC GGTCCAGGAC ATCATCAGAC TGCACTGATA CCAATTACAT CCTGCGCCAA 
AGCAGTTTCA ACAGCCAAGA AGGCAATTCC TACAGGCTAC AGGAGTCTCT GGAGCAGGGG 
GGCGACGAGT CCATTTCTCC TACTTCCCCA ACCGCCCTGG CTCCCCGTGT CCGCAGTCAC 
TCCTTCTACG TGAGCCACTC TTCCAAAGAC AGGAGTGGGG CGGACCGTGG AGAGGGCTTC 
TTTAAAGACA AGTTGTTCAG CCTCCACCGG GCCAACAGCT CACAGTCTGT GTCCTCAGGA 
GCAGGTCCCA AAGAGTCCAA GCCAACCCCT TTGAATACTT TATCCGTGCA GCAGCAGCTC 
CGCCCTTCTT CCTGCATAGA CATCTATGTT TCTGCCTCAG AGGACGTCCC GCCCACTGAG 
AGCTTCTTGG ATTCGGTTCG GACAGTTCCA ACTCTGGCTC GAGACTCATC TCTTCATTCG 
GAGATCATGG AGGCGGTGCT CTCAGGGGGA CGGGACTGTA GTGGAAGGGC AGGCGGCAGC 
GAAAGACAGT CGGATGGTAC AGTCCTGTTT GAGGACAGCG CCGCCGCTGA CCTATCGCTC 
TGCTCTGCCC ACCTGCTGCC GGATTCCTTG CCCCCCTGGG ACCTGGACCC CTCACCGCCA 
CCATCAGCTG GTGTACTAGA GCGGTCCAAA AGTAGTCGCT TCCTGTCTAC AGCAGGGCCT 
CTGTTTCTGG ACGAGCCTCA TTTGGTTAAG TCACACAGTC TGATGTTCAC ATCACGTGGG 
TACTATGGTG GCATGGGTGT GCAGGTGAAG GCGGCCGAAT ACACCAGCAT CACAGATTGC 
ATCGACACCC GTTGCGTCTC AACCCCTTAC CCTGTGCCAG AGCGGTCAGA CTCACCTGGA 
GGATCTTTCA CCTTCGATAA GCCTCAAGAC CTTGGCGTCT CGCACCCTGA GAGAGACGCT 
GAGCTCAGCC ACGCTGAGTC TGACTTAGAG GAGCCAGCAG AGGGTTCGGG AGACACATGT 
AAGGTGGGTC AGAGTAGCAG TAGTGGGGTA ATAGGTGCAG ACCTGGGCCT GGGCCTCGCA 
CTAGGCCCGT TTTGCTCACC CATCTCTAGA CTAGAGCGAG CAAACAGCTG CTCCTCTTCA 
GAGGAGTCGC ACTCCAACAT CTACTCTCGA AAGAGCTTCT CAATAAGCGA GAGAATGGAC 
AAGGGCCGGG GGAGCTCCAG GAACCCTTTC CAGAAGGCCA GAACCGGGGC GAGGCTGGAG 
GGTAAGACAG ACTCACTGTC TATGAGGAAA ATGGCCAAAC CGTCAGCTTT CAGAAGTTTT 
GACAGCAGAC ACAACTACAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm3 ( XM_009301557.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299832.1 Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X1, mRNA.

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CloneID	ODa44718
Clone ID Related Accession (Same CDS sequence)	XM_009301557.3 , XM_009301557.2
Accession Version	XM_009301557.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transient receptor potential cation channel subfamily M member 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301557.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAAAA GAAAATGGGA GGGGAGGAGA GAAGGCTTCA AGGTGAGCTC CACGAGGGAG 
GAGGCACAGG GGCCTGGACG CCCCTCCACC AGAGGGCGCT TCTCCGAATC TTGGAAGAGA 
CTGAGCTCCA GACAGGGGTC TACCAAGAGG TCAGGACTGG CCTCCCAGCA AGCTGCGGCA 
CAGAAATCAT GGATAGAAAG AGCGTTCAGC AAGAGAGAAT GTGTGCACAT TATTGTAAGC 
ACCAAAGACC ACCATAGGTG CTGTTGTGGC CGTCTAATTG GTCAACATGT TGGCCTGCCC 
CCCAGCATCT CATCCAATCA GAATGAGAAG TCAGAACGCG TGCCAAAGAA TGACAGCTTG 
TCAGAGAAGT GGTCCATCAG CAAGCACACC CAGCTCAGTC CCACCGACGC TTTTGGGACC 
ATCGAGTTCC AGGGAGGAGG ACACTCTAAT AAAGCAATGT ATGTACGAGT GTCTTACGAC 
ACAAAACCAG ATTTACTGCT GCATCTGATG ACAAAGGAGT GGCAACTGGA GCTTCCCAAG 
CTGCTCATCT CTGTTCACGG AGGACTTCAA AACTTTGAGT TGCAGCCCAA ACTAAAGCAA 
GTCTTCGGCA AAGGTCTTAT AAAGGCTGCA ATGACTACCG GAGCCTGGAT CTTCACTGGA 
GGAGTTAATA CTGGAGTCAT CAGACATGTT GGTGATGCTC TGAAAGATCA TGCTTCCAAA 
TCAAGAGGGA AGATCTGCAC TATCGGCATT GCCCCCTGGG GGATTGTGGA GAACCAGGAA 
GACCTGGTGG GCAAAGATGT GGTCCGGCCA TACCAGACGA TGTCTAACCC TCTTAGCAAG 
CTAACAGTAC TCAACAGCCT ACACTCACAC TTCATTTTGG CTGATAATGG CACCACCGGA 
AAGTACGGAG CAGAAGTCAA ACTGCGCAGG CAGCTGGAGA AGCACATCTC TCTGCAAAAG 
ATTAACACAC GGATTGGACA GGGTGTTCCA GTAGTGGCTC TGATAGTGGA GGGGGGTCCG 
AATGTCATCT CTATCGTCCT GGAGTACCTA AGAGACACCC CTCCGGTGCC TGTTGTGGTG 
TGTGACGGCA GCGGTCGAGC CTCTGACATC CTGGCCTTTG GGCACAAGTA CTCAGAGGAG 
GGCGGAATCA TAAACGAATC TTTGAGAGAC CAGTTATTAG TCACCATTCA GAAGACGTTC 
ACATACAGCC GGACGCAGGC CCAGCATCTC TTCATCATTC TGATGGAGTG CATGAAGAAG 
AAAGAACTGA TCACAGTGTT TCGGATGGGA TCAGAGGGAC ACCAGGACAT CGATCTAGCC 
ATTCTAACAG CTCTTCTGAA AGGAGCGAAT GCATCAGCTC CAGATCAGCT AAGTCTGGCT 
TTGGCCTGGA ACAGAGTTGA CATCGCCCGG AGCCAGATCT TCATTTACGG ACAGCAGTGG 
CCTGTTGGCT CTCTGGAGCA GTCTATGCTG GACGCGCTGG TGCTGGACCG AGTGGACTTT 
GTAAAGCTGT TGATTGAGAA CGGAGTGAGC ATGCACCGTT TCCTGACACT CTCCAGACTT 
GAAGAGCTTT ATAATACGAG GCACGGACCA TCCAACACCT TGTACCACCT TGTCAGAGAT 
GTGAAAAAGC AAGAATATCC AGGGTTCAGT TGGATCTACC TCAAGGGTAA CCTGCCCCCC 
GACTACCGCA TTAGCCTGAT TGACATCGGA TTGGTTATTG AATACCTCAT GGGTGGAGCC 
TATCGCTGCA ATTACACCAG GAAGAGATTT CGGACCCTCT ATCACAACCT CTTTGGACCC 
AAAAGACCAA AAGCCCTGAA GCTGCTTGGA ATGGAGGATG ACATGCCCAT TAGACGAGGG 
CGTCAGAAGA CTACGCGGAA GCGAGAGGAG GAAGTAGATA TTGACCTGGA TGACCCAGAA 
ATCAATCATT TCCCTTTCCC ATTCCATGAG CTGATGGTTT GGGCTGTGCT GATGAAACGG 
CAGAAGATGG CACTGTTCTT CTGGCAGCAT GGAGAGGAGG CCATGGCTAA AGCGCTGGTG 
GCCTGCAAGC TCTGTAAGGC AATGGCTCAC GAAGCTTCTG AGAACGACAT GGTCGATGAC 
ATTTCACAGG AGCTCAATCA AAACTCAAGG GAGTTTGGTC AGCTGGCTGT TGAGCTGCTC 
GATCAGTCCT ATAAGCAGGA TGAACAGATG GCTATGAAGC TGTTGACCTA CGAGCTGAAG 
AACTGGAGTA ATGCCACCTG TCTGCAGCTG GCAGTGGCTG CCAAGCACCG AGACTTCATC 
GCCCACACCT GTAGCCAGAT GCTGCTCACA GACATGTGGA TGGGACGTCT GCGCATGCGC 
AAGAACTCTG GCCTGAAGGT TATCTTGGGC CTGCTTCTCC CCCCTTCCAT ATTGAGTCTG 
GAGTTTAAAA ATAAAGACGA GATGTCATAC ATGCCTCAGG ATCAGGACAC ATATCTGCAG 
GAGAAGGATG TGGATGAGCC CGAGAAACAA GCTAAGGAGA AAGAGGAGGA GGACATGGAG 
TTCACAGTAA GATCTTACTG TGAGACGCAG TACAACTCCG TGGCTATGTT GGGAAACGTC 
AGTTCAGAAG CATCGCGCAA GAAACAGGTG GAGGAGGTTC AGAACCGGCA TCGGCTCATT 
CCATTGGGTC GCAAAATATA TGAGTTTTAC AACGCCCCTA TCGTCAAGTT CTGGTTTCAC 
ACGCTGGCGT ATGTAGGATA CTTGATGCTC TTCAACTATA TTGTGCTGGT GAAGATGGAC 
CTGTGGCCTT CTCCTCAGGA GTGGATTGTT ATTGCCTACA TATTCACCAA TGGCATCGAG 
AAGATGAGAG AGATCCTGAT GTCCGAACCT GGGAAGCTGC TTCAGAAGGT GAAAGTGTGG 
TTACAGGAGT ACTGGAACAT CACTGACCTC ATGGCCATCC TCATCTTCTC CATCGGGATG 
GTGCTTCGTC TGCAGGACCC GCCGCTGATG AGCTACGGCA GGGTCATTTA CTGCGTCAAT 
ATCATTTATT GGTACATACG ACTCCTCGAC ATCTTCGGAG TGAACAAATA CCTGGGTCCA 
TACGTCATGA TGATCGGCAA GATGATGATT GACATGATGT ATTTTGTGAT CATCATGTTG 
GTGGTGCTGA TGAGTTTCGG TGTGGCGCGT CAGGCCATCC TTAATCCCAA TGAAGACCCA 
TCCTGGATGC TGGCACGCAA TATCTTCTTC ATGCCATACT GGATGATCTA CGGAGAGGTG 
TTTGCTGACC AAATCGACCC TCCTTGTGGT CAGAACATCA CCACAGAAGA AGGAGTTGTC 
ATGCCTCTTC CACCCTGCAA AACTGGAGCT TGGATTGTCC CTGCCATAAT GGCCTGCTAC 
CTGCTGGTGG CCAACATCCT GCTAGTTAAC TTGCTCATTG CTGTCTTTAA TAACACTTTC 
TTTGAAGTCA AGTCCATTTC CAACCAGGTG TGGAAGTTTC AGAGGTATCA GCTGATCATG 
ACCTTCCATG AACGCCCGGT CCTTCCTCCT CCACTCATCA TCTTCAGCCA CATAACTATG 
GTTCTCAAAC ACCTGTGCTG TCGCTGGAGG AAGCACGATG AGGATGAACG GGACTATGGG 
CTGAAGCTTT TCATCACTGA AGATGAACTG AAGAAAGTGC ATGACTTTGA AGAGCAGTGC 
ATGGAGGAGT ATTTCAGAGA GAAAGATGAC CGCTTCAATT CCTCCAATGA CGAAAGGATA 
CGTGTGACAT CTGAAAGGGT GGAGAACATG GCGATGCGAT TAGAGGAGGT GAATGAGAGG 
GAACATTTTA TGAAAGCGTC CTTGCAGACT GTAGACATCC GGCTCGCCCA GATGGAGGAG 
ATGATCGGCC GCATTGCTGT GGCCCTAGAG AGAGTTGCTG GAATGGACAG AGGCGAAGTT 
AACAAAGCCC GGTCCAGGAC ATCATCAGAC TGCACTGATA CCAATTACAT CCTGCGCCAA 
AGCAGTTTCA ACAGCCAAGA AGGCAATTCC TACAGGCTAC AGGAGTCTCT GGAGCAGGGG 
GGCGACGAGT CCATTTCTCC TACTTCCCCA ACCGCCCTGG CTCCCCGTGT CCGCAGTCAC 
TCCTTCTACG TGAGCCACTC TTCCAAAGAC AGGAGTGGGG CGGACCGTGG AGAGGGCTTC 
TTTAAAGACA AGTTGTTCAG CCTCCACCGG GCCAACAGCT CACAGTCTGT GTCCTCAGGA 
GCAGGTCCCA AAGAGTCCAA GCCAACCCCT TTGAATACTT TATCCGTGCA GCAGCAGCTC 
CGCCCTTCTT CCTGCATAGA CATCTATGTT TCTGCCTCAG AGGACGTCCC GCCCACTGAG 
AGCTTCTTGG ATTCGGTTCG GACAGTTCCA ACTCTGGCTC GAGACTCATC TCTTCATTCG 
GAGATCATGG AGGCGGTGCT CTCAGGGGGA CGGGACTGTA GTGGAAGGGC AGGCGGCAGC 
GAAAGACAGT CGGATGGTAC AGTCCTGTTT GAGGACAGCG CCGCCGCTGA CCTATCGCTC 
TGCTCTGCCC ACCTGCTGCC GGATTCCTTG CCCCCCTGGG ACCTGGACCC CTCACCGCCA 
CCATCAGCTG GTGTACTAGA GCGGTCCAAA AGTAGTCGCT TCCTGTCTAC AGCAGGGCCT 
CTGTTTCTGG ACGAGCCTCA TTTGGTTAAG TCACACAGTC TGATGTTCAC ATCACGTGGG 
TACTATGGTG GCATGGGTGT GCAGGTGAAG GCGGCCGAAT ACACCAGCAT CACAGATTGC 
ATCGACACCC GTTGCGTCTC AACCCCTTAC CCTGTGCCAG AGCGGTCAGA CTCACCTGGA 
GGATCTTTCA CCTTCGATAA GCCTCAAGAC CTTGGCGTCT CGCACCCTGA GAGAGACGCT 
GAGCTCAGCC ACGCTGAGTC TGACTTAGAG GAGCCAGCAG AGGGTTCGGG AGACACATGT 
AAGGTGGGTC AGAGTAGCAG TAGTGGGGTA ATAGGTGCAG ACCTGGGCCT GGGCCTCGCA 
CTAGGCCCGT TTTGCTCACC CATCTCTAGA CTAGAGCGAG CAAACAGCTG CTCCTCTTCA 
GAGGAGTCGC ACTCCAACAT CTACTCTCGA AAGAGCTTCT CAATAAGCGA GAGAATGGAC 
AAGGGCCGGG GGAGCTCCAG GAACCCTTTC CAGAAGGCCA GAACCGGGGC GAGGCTGGAG 
GGTAAGACAG ACTCACTGTC TATGAGGAAA ATGGCCAAAC CGTCAGCTTT CAGAAGTTTT 
GACAGCAGAC ACAACTACAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299832.1
CDS	338..5581
Translation	MTKRKWEGRREGFKVSSTREEAQGPGRPSTRGRFSESWKRLSSRQGSTKRSGLASQQAAAQKSWIERAFSKRECVHIIVSTKDHHRCCCGRLIGQHVGLPPSISSNQNEKSERVPKNDSLSEKWSISKHTQLSPTDAFGTIEFQGGGHSNKAMYVRVSYDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLVGKDVVRPYQTMSNPLSKLTVLNSLHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGIINESLRDQLLVTIQKTFTYSRTQAQHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQSMLDALVLDRVDFVKLLIENGVSMHRFLTLSRLEELYNTRHGPSNTLYHLVRDVKKQEYPGFSWIYLKGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDMPIRRGRQKTTRKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNQNSREFGQLAVELLDQSYKQDEQMAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGLLLPPSILSLEFKNKDEMSYMPQDQDTYLQEKDVDEPEKQAKEKEEEDMEFTVRSYCETQYNSVAMLGNVSSEASRKKQVEEVQNRHRLIPLGRKIYEFYNAPIVKFWFHTLAYVGYLMLFNYIVLVKMDLWPSPQEWIVIAYIFTNGIEKMREILMSEPGKLLQKVKVWLQEYWNITDLMAILIFSIGMVLRLQDPPLMSYGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDPPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHITMVLKHLCCRWRKHDEDERDYGLKLFITEDELKKVHDFEEQCMEEYFREKDDRFNSSNDERIRVTSERVENMAMRLEEVNEREHFMKASLQTVDIRLAQMEEMIGRIAVALERVAGMDRGEVNKARSRTSSDCTDTNYILRQSSFNSQEGNSYRLQESLEQGGDESISPTSPTALAPRVRSHSFYVSHSSKDRSGADRGEGFFKDKLFSLHRANSSQSVSSGAGPKESKPTPLNTLSVQQQLRPSSCIDIYVSASEDVPPTESFLDSVRTVPTLARDSSLHSEIMEAVLSGGRDCSGRAGGSERQSDGTVLFEDSAAADLSLCSAHLLPDSLPPWDLDPSPPPSAGVLERSKSSRFLSTAGPLFLDEPHLVKSHSLMFTSRGYYGGMGVQVKAAEYTSITDCIDTRCVSTPYPVPERSDSPGGSFTFDKPQDLGVSHPERDAELSHAESDLEEPAEGSGDTCKVGQSSSSGVIGADLGLGLALGPFCSPISRLERANSCSSSEESHSNIYSRKSFSISERMDKGRGSSRNPFQKARTGARLEGKTDSLSMRKMAKPSAFRSFDSRHNYT

Target ORF information:

RefSeq Version	XM_009301557.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301557.2

ORF Insert Sequence:

ATGACAAAAA GAAAATGGGA GGGGAGGAGA GAAGGCTTCA AGGTGAGCTC CACGAGGGAG 
GAGGCACAGG GGCCTGGACG CCCCTCCACC AGAGGGCGCT TCTCCGAATC TTGGAAGAGA 
CTGAGCTCCA GACAGGGGTC TACCAAGAGG TCAGGACTGG CCTCCCAGCA AGCTGCGGCA 
CAGAAATCAT GGATAGAAAG AGCGTTCAGC AAGAGAGAAT GTGTGCACAT TATTGTAAGC 
ACCAAAGACC ACCATAGGTG CTGTTGTGGC CGTCTAATTG GTCAACATGT TGGCCTGCCC 
CCCAGCATCT CATCCAATCA GAATGAGAAG TCAGAACGCG TGCCAAAGAA TGACAGCTTG 
TCAGAGAAGT GGTCCATCAG CAAGCACACC CAGCTCAGTC CCACCGACGC TTTTGGGACC 
ATCGAGTTCC AGGGAGGAGG ACACTCTAAT AAAGCAATGT ATGTACGAGT GTCTTACGAC 
ACAAAACCAG ATTTACTGCT GCATCTGATG ACAAAGGAGT GGCAACTGGA GCTTCCCAAG 
CTGCTCATCT CTGTTCACGG AGGACTTCAA AACTTTGAGT TGCAGCCCAA ACTAAAGCAA 
GTCTTCGGCA AAGGTCTTAT AAAGGCTGCA ATGACTACCG GAGCCTGGAT CTTCACTGGA 
GGAGTTAATA CTGGAGTCAT CAGACATGTT GGTGATGCTC TGAAAGATCA TGCTTCCAAA 
TCAAGAGGGA AGATCTGCAC TATCGGCATT GCCCCCTGGG GGATTGTGGA GAACCAGGAA 
GACCTGGTGG GCAAAGATGT GGTCCGGCCA TACCAGACGA TGTCTAACCC TCTTAGCAAG 
CTAACAGTAC TCAACAGCCT ACACTCACAC TTCATTTTGG CTGATAATGG CACCACCGGA 
AAGTACGGAG CAGAAGTCAA ACTGCGCAGG CAGCTGGAGA AGCACATCTC TCTGCAAAAG 
ATTAACACAC GGATTGGACA GGGTGTTCCA GTAGTGGCTC TGATAGTGGA GGGGGGTCCG 
AATGTCATCT CTATCGTCCT GGAGTACCTA AGAGACACCC CTCCGGTGCC TGTTGTGGTG 
TGTGACGGCA GCGGTCGAGC CTCTGACATC CTGGCCTTTG GGCACAAGTA CTCAGAGGAG 
GGCGGAATCA TAAACGAATC TTTGAGAGAC CAGTTATTAG TCACCATTCA GAAGACGTTC 
ACATACAGCC GGACGCAGGC CCAGCATCTC TTCATCATTC TGATGGAGTG CATGAAGAAG 
AAAGAACTGA TCACAGTGTT TCGGATGGGA TCAGAGGGAC ACCAGGACAT CGATCTAGCC 
ATTCTAACAG CTCTTCTGAA AGGAGCGAAT GCATCAGCTC CAGATCAGCT AAGTCTGGCT 
TTGGCCTGGA ACAGAGTTGA CATCGCCCGG AGCCAGATCT TCATTTACGG ACAGCAGTGG 
CCTGTTGGCT CTCTGGAGCA GTCTATGCTG GACGCGCTGG TGCTGGACCG AGTGGACTTT 
GTAAAGCTGT TGATTGAGAA CGGAGTGAGC ATGCACCGTT TCCTGACACT CTCCAGACTT 
GAAGAGCTTT ATAATACGAG GCACGGACCA TCCAACACCT TGTACCACCT TGTCAGAGAT 
GTGAAAAAGC AAGAATATCC AGGGTTCAGT TGGATCTACC TCAAGGGTAA CCTGCCCCCC 
GACTACCGCA TTAGCCTGAT TGACATCGGA TTGGTTATTG AATACCTCAT GGGTGGAGCC 
TATCGCTGCA ATTACACCAG GAAGAGATTT CGGACCCTCT ATCACAACCT CTTTGGACCC 
AAAAGACCAA AAGCCCTGAA GCTGCTTGGA ATGGAGGATG ACATGCCCAT TAGACGAGGG 
CGTCAGAAGA CTACGCGGAA GCGAGAGGAG GAAGTAGATA TTGACCTGGA TGACCCAGAA 
ATCAATCATT TCCCTTTCCC ATTCCATGAG CTGATGGTTT GGGCTGTGCT GATGAAACGG 
CAGAAGATGG CACTGTTCTT CTGGCAGCAT GGAGAGGAGG CCATGGCTAA AGCGCTGGTG 
GCCTGCAAGC TCTGTAAGGC AATGGCTCAC GAAGCTTCTG AGAACGACAT GGTCGATGAC 
ATTTCACAGG AGCTCAATCA AAACTCAAGG GAGTTTGGTC AGCTGGCTGT TGAGCTGCTC 
GATCAGTCCT ATAAGCAGGA TGAACAGATG GCTATGAAGC TGTTGACCTA CGAGCTGAAG 
AACTGGAGTA ATGCCACCTG TCTGCAGCTG GCAGTGGCTG CCAAGCACCG AGACTTCATC 
GCCCACACCT GTAGCCAGAT GCTGCTCACA GACATGTGGA TGGGACGTCT GCGCATGCGC 
AAGAACTCTG GCCTGAAGGT TATCTTGGGC CTGCTTCTCC CCCCTTCCAT ATTGAGTCTG 
GAGTTTAAAA ATAAAGACGA GATGTCATAC ATGCCTCAGG ATCAGGACAC ATATCTGCAG 
GAGAAGGATG TGGATGAGCC CGAGAAACAA GCTAAGGAGA AAGAGGAGGA GGACATGGAG 
TTCACAGTAA GATCTTACTG TGAGACGCAG TACAACTCCG TGGCTATGTT GGGAAACGTC 
AGTTCAGAAG CATCGCGCAA GAAACAGGTG GAGGAGGTTC AGAACCGGCA TCGGCTCATT 
CCATTGGGTC GCAAAATATA TGAGTTTTAC AACGCCCCTA TCGTCAAGTT CTGGTTTCAC 
ACGCTGGCGT ATGTAGGATA CTTGATGCTC TTCAACTATA TTGTGCTGGT GAAGATGGAC 
CTGTGGCCTT CTCCTCAGGA GTGGATTGTT ATTGCCTACA TATTCACCAA TGGCATCGAG 
AAGATGAGAG AGATCCTGAT GTCCGAACCT GGGAAGCTGC TTCAGAAGGT GAAAGTGTGG 
TTACAGGAGT ACTGGAACAT CACTGACCTC ATGGCCATCC TCATCTTCTC CATCGGGATG 
GTGCTTCGTC TGCAGGACCC GCCGCTGATG AGCTACGGCA GGGTCATTTA CTGCGTCAAT 
ATCATTTATT GGTACATACG ACTCCTCGAC ATCTTCGGAG TGAACAAATA CCTGGGTCCA 
TACGTCATGA TGATCGGCAA GATGATGATT GACATGATGT ATTTTGTGAT CATCATGTTG 
GTGGTGCTGA TGAGTTTCGG TGTGGCGCGT CAGGCCATCC TTAATCCCAA TGAAGACCCA 
TCCTGGATGC TGGCACGCAA TATCTTCTTC ATGCCATACT GGATGATCTA CGGAGAGGTG 
TTTGCTGACC AAATCGACCC TCCTTGTGGT CAGAACATCA CCACAGAAGA AGGAGTTGTC 
ATGCCTCTTC CACCCTGCAA AACTGGAGCT TGGATTGTCC CTGCCATAAT GGCCTGCTAC 
CTGCTGGTGG CCAACATCCT GCTAGTTAAC TTGCTCATTG CTGTCTTTAA TAACACTTTC 
TTTGAAGTCA AGTCCATTTC CAACCAGGTG TGGAAGTTTC AGAGGTATCA GCTGATCATG 
ACCTTCCATG AACGCCCGGT CCTTCCTCCT CCACTCATCA TCTTCAGCCA CATAACTATG 
GTTCTCAAAC ACCTGTGCTG TCGCTGGAGG AAGCACGATG AGGATGAACG GGACTATGGG 
CTGAAGCTTT TCATCACTGA AGATGAACTG AAGAAAGTGC ATGACTTTGA AGAGCAGTGC 
ATGGAGGAGT ATTTCAGAGA GAAAGATGAC CGCTTCAATT CCTCCAATGA CGAAAGGATA 
CGTGTGACAT CTGAAAGGGT GGAGAACATG GCGATGCGAT TAGAGGAGGT GAATGAGAGG 
GAACATTTTA TGAAAGCGTC CTTGCAGACT GTAGACATCC GGCTCGCCCA GATGGAGGAG 
ATGATCGGCC GCATTGCTGT GGCCCTAGAG AGAGTTGCTG GAATGGACAG AGGCGAAGTT 
AACAAAGCCC GGTCCAGGAC ATCATCAGAC TGCACTGATA CCAATTACAT CCTGCGCCAA 
AGCAGTTTCA ACAGCCAAGA AGGCAATTCC TACAGGCTAC AGGAGTCTCT GGAGCAGGGG 
GGCGACGAGT CCATTTCTCC TACTTCCCCA ACCGCCCTGG CTCCCCGTGT CCGCAGTCAC 
TCCTTCTACG TGAGCCACTC TTCCAAAGAC AGGAGTGGGG CGGACCGTGG AGAGGGCTTC 
TTTAAAGACA AGTTGTTCAG CCTCCACCGG GCCAACAGCT CACAGTCTGT GTCCTCAGGA 
GCAGGTCCCA AAGAGTCCAA GCCAACCCCT TTGAATACTT TATCCGTGCA GCAGCAGCTC 
CGCCCTTCTT CCTGCATAGA CATCTATGTT TCTGCCTCAG AGGACGTCCC GCCCACTGAG 
AGCTTCTTGG ATTCGGTTCG GACAGTTCCA ACTCTGGCTC GAGACTCATC TCTTCATTCG 
GAGATCATGG AGGCGGTGCT CTCAGGGGGA CGGGACTGTA GTGGAAGGGC AGGCGGCAGC 
GAAAGACAGT CGGATGGTAC AGTCCTGTTT GAGGACAGCG CCGCCGCTGA CCTATCGCTC 
TGCTCTGCCC ACCTGCTGCC GGATTCCTTG CCCCCCTGGG ACCTGGACCC CTCACCGCCA 
CCATCAGCTG GTGTACTAGA GCGGTCCAAA AGTAGTCGCT TCCTGTCTAC AGCAGGGCCT 
CTGTTTCTGG ACGAGCCTCA TTTGGTTAAG TCACACAGTC TGATGTTCAC ATCACGTGGG 
TACTATGGTG GCATGGGTGT GCAGGTGAAG GCGGCCGAAT ACACCAGCAT CACAGATTGC 
ATCGACACCC GTTGCGTCTC AACCCCTTAC CCTGTGCCAG AGCGGTCAGA CTCACCTGGA 
GGATCTTTCA CCTTCGATAA GCCTCAAGAC CTTGGCGTCT CGCACCCTGA GAGAGACGCT 
GAGCTCAGCC ACGCTGAGTC TGACTTAGAG GAGCCAGCAG AGGGTTCGGG AGACACATGT 
AAGGTGGGTC AGAGTAGCAG TAGTGGGGTA ATAGGTGCAG ACCTGGGCCT GGGCCTCGCA 
CTAGGCCCGT TTTGCTCACC CATCTCTAGA CTAGAGCGAG CAAACAGCTG CTCCTCTTCA 
GAGGAGTCGC ACTCCAACAT CTACTCTCGA AAGAGCTTCT CAATAAGCGA GAGAATGGAC 
AAGGGCCGGG GGAGCTCCAG GAACCCTTTC CAGAAGGCCA GAACCGGGGC GAGGCTGGAG 
GGTAAGACAG ACTCACTGTC TATGAGGAAA ATGGCCAAAC CGTCAGCTTT CAGAAGTTTT 
GACAGCAGAC ACAACTACAC GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm3 ( XM_009301558.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299833.1 Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa44719
Clone ID Related Accession (Same CDS sequence)	XM_009301558.2 , XM_009301558.3
Accession Version	XM_009301558.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transient receptor potential cation channel subfamily M member 3 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301558.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAAAA GAAAATGGGA GGGGAGGAGA GAAGGCTTCA AGGTGAGCTC CACGAGGGAG 
GAGGCACAGG GGCCTGGACG CCCCTCCACC AGAGGGCGCT TCTCCGAATC TTGGAAGAGA 
CTGAGCTCCA GACAGGGGTC TACCAAGAGG TCAGGACTGG CCTCCCAGCA AGCTGCGGCA 
CAGAAATCAT GGATAGAAAG AGCGTTCAGC AAGAGAGAAT GTGTGCACAT TATTGTAAGC 
ACCAAAGACC ACCATAGGTG CTGTTGTGGC CGTCTAATTG GTCAACATGT TGGCCTGCCC 
CCCAGCATCT CATCCAATCA GAATGAGAAG TCAGAACGCG TGCCAAAGAA TGACAGCTTG 
TCAGAGAAGT GGTCCATCAG CAAGCACACC CAGCTCAGTC CCACCGACGC TTTTGGGACC 
ATCGAGTTCC AGGGAGGAGG ACACTCTAAT AAAGCAATGT ATGTACGAGT GTCTTACGAC 
ACAAAACCAG ATTTACTGCT GCATCTGATG ACAAAGGAGT GGCAACTGGA GCTTCCCAAG 
CTGCTCATCT CTGTTCACGG AGGACTTCAA AACTTTGAGT TGCAGCCCAA ACTAAAGCAA 
GTCTTCGGCA AAGGTCTTAT AAAGGCTGCA ATGACTACCG GAGCCTGGAT CTTCACTGGA 
GGAGTTAATA CTGGAGTCAT CAGACATGTT GGTGATGCTC TGAAAGATCA TGCTTCCAAA 
TCAAGAGGGA AGATCTGCAC TATCGGCATT GCCCCCTGGG GGATTGTGGA GAACCAGGAA 
GACCTGGTGG GCAAAGATGT GGTCCGGCCA TACCAGACGA TGTCTAACCC TCTTAGCAAG 
CTAACAGTAC TCAACAGCCT ACACTCACAC TTCATTTTGG CTGATAATGG CACCACCGGA 
AAGTACGGAG CAGAAGTCAA ACTGCGCAGG CAGCTGGAGA AGCACATCTC TCTGCAAAAG 
ATTAACACAC GGATTGGACA GGGTGTTCCA GTAGTGGCTC TGATAGTGGA GGGGGGTCCG 
AATGTCATCT CTATCGTCCT GGAGTACCTA AGAGACACCC CTCCGGTGCC TGTTGTGGTG 
TGTGACGGCA GCGGTCGAGC CTCTGACATC CTGGCCTTTG GGCACAAGTA CTCAGAGGAG 
GGCGGAATCA TAAACGAATC TTTGAGAGAC CAGTTATTAG TCACCATTCA GAAGACGTTC 
ACATACAGCC GGACGCAGGC CCAGCATCTC TTCATCATTC TGATGGAGTG CATGAAGAAG 
AAAGAACTGA TCACAGTGTT TCGGATGGGA TCAGAGGGAC ACCAGGACAT CGATCTAGCC 
ATTCTAACAG CTCTTCTGAA AGGAGCGAAT GCATCAGCTC CAGATCAGCT AAGTCTGGCT 
TTGGCCTGGA ACAGAGTTGA CATCGCCCGG AGCCAGATCT TCATTTACGG ACAGCAGTGG 
CCTGTTGGCT CTCTGGAGCA GTCTATGCTG GACGCGCTGG TGCTGGACCG AGTGGACTTT 
GTAAAGCTGT TGATTGAGAA CGGAGTGAGC ATGCACCGTT TCCTGACACT CTCCAGACTT 
GAAGAGCTTT ATAATACGAG GCACGGACCA TCCAACACCT TGTACCACCT TGTCAGAGAT 
GTGAAAAAGG GTAACCTGCC CCCCGACTAC CGCATTAGCC TGATTGACAT CGGATTGGTT 
ATTGAATACC TCATGGGTGG AGCCTATCGC TGCAATTACA CCAGGAAGAG ATTTCGGACC 
CTCTATCACA ACCTCTTTGG ACCCAAAAGA CCAAAAGCCC TGAAGCTGCT TGGAATGGAG 
GATGACATGC CCATTAGACG AGGGCGTCAG AAGACTACGC GGAAGCGAGA GGAGGAAGTA 
GATATTGACC TGGATGACCC AGAAATCAAT CATTTCCCTT TCCCATTCCA TGAGCTGATG 
GTTTGGGCTG TGCTGATGAA ACGGCAGAAG ATGGCACTGT TCTTCTGGCA GCATGGAGAG 
GAGGCCATGG CTAAAGCGCT GGTGGCCTGC AAGCTCTGTA AGGCAATGGC TCACGAAGCT 
TCTGAGAACG ACATGGTCGA TGACATTTCA CAGGAGCTCA ATCAAAACTC AAGGGAGTTT 
GGTCAGCTGG CTGTTGAGCT GCTCGATCAG TCCTATAAGC AGGATGAACA GATGGCTATG 
AAGCTGTTGA CCTACGAGCT GAAGAACTGG AGTAATGCCA CCTGTCTGCA GCTGGCAGTG 
GCTGCCAAGC ACCGAGACTT CATCGCCCAC ACCTGTAGCC AGATGCTGCT CACAGACATG 
TGGATGGGAC GTCTGCGCAT GCGCAAGAAC TCTGGCCTGA AGGTTATCTT GGGCCTGCTT 
CTCCCCCCTT CCATATTGAG TCTGGAGTTT AAAAATAAAG ACGAGATGTC ATACATGCCT 
CAGGATCAGG ACACATATCT GCAGGAGAAG GATGTGGATG AGCCCGAGAA ACAAGCTAAG 
GAGAAAGAGG AGGAGGACAT GGAGTTCACA GTAAGATCTT ACTGTGAGAC GCAGTACAAC 
TCCGTGGCTA TGTTGGGAAA CGTCAGTTCA GAAGCATCGC GCAAGAAACA GGTGGAGGAG 
GTTCAGAACC GGCATCGGCT CATTCCATTG GGTCGCAAAA TATATGAGTT TTACAACGCC 
CCTATCGTCA AGTTCTGGTT TCACACGCTG GCGTATGTAG GATACTTGAT GCTCTTCAAC 
TATATTGTGC TGGTGAAGAT GGACCTGTGG CCTTCTCCTC AGGAGTGGAT TGTTATTGCC 
TACATATTCA CCAATGGCAT CGAGAAGATG AGAGAGATCC TGATGTCCGA ACCTGGGAAG 
CTGCTTCAGA AGGTGAAAGT GTGGTTACAG GAGTACTGGA ACATCACTGA CCTCATGGCC 
ATCCTCATCT TCTCCATCGG GATGGTGCTT CGTCTGCAGG ACCCGCCGCT GATGAGCTAC 
GGCAGGGTCA TTTACTGCGT CAATATCATT TATTGGTACA TACGACTCCT CGACATCTTC 
GGAGTGAACA AATACCTGGG TCCATACGTC ATGATGATCG GCAAGATGAT GATTGACATG 
ATGTATTTTG TGATCATCAT GTTGGTGGTG CTGATGAGTT TCGGTGTGGC GCGTCAGGCC 
ATCCTTAATC CCAATGAAGA CCCATCCTGG ATGCTGGCAC GCAATATCTT CTTCATGCCA 
TACTGGATGA TCTACGGAGA GGTGTTTGCT GACCAAATCG ACCCTCCTTG TGGTCAGAAC 
ATCACCACAG AAGAAGGAGT TGTCATGCCT CTTCCACCCT GCAAAACTGG AGCTTGGATT 
GTCCCTGCCA TAATGGCCTG CTACCTGCTG GTGGCCAACA TCCTGCTAGT TAACTTGCTC 
ATTGCTGTCT TTAATAACAC TTTCTTTGAA GTCAAGTCCA TTTCCAACCA GGTGTGGAAG 
TTTCAGAGGT ATCAGCTGAT CATGACCTTC CATGAACGCC CGGTCCTTCC TCCTCCACTC 
ATCATCTTCA GCCACATAAC TATGGTTCTC AAACACCTGT GCTGTCGCTG GAGGAAGCAC 
GATGAGGATG AACGGGACTA TGGGCTGAAG CTTTTCATCA CTGAAGATGA ACTGAAGAAA 
GTGCATGACT TTGAAGAGCA GTGCATGGAG GAGTATTTCA GAGAGAAAGA TGACCGCTTC 
AATTCCTCCA ATGACGAAAG GATACGTGTG ACATCTGAAA GGGTGGAGAA CATGGCGATG 
CGATTAGAGG AGGTGAATGA GAGGGAACAT TTTATGAAAG CGTCCTTGCA GACTGTAGAC 
ATCCGGCTCG CCCAGATGGA GGAGATGATC GGCCGCATTG CTGTGGCCCT AGAGAGAGTT 
GCTGGAATGG ACAGAGGCGA AGTTAACAAA GCCCGGTCCA GGACATCATC AGACTGCACT 
GATACCAATT ACATCCTGCG CCAAAGCAGT TTCAACAGCC AAGAAGGCAA TTCCTACAGG 
CTACAGGAGT CTCTGGAGCA GGGGGGCGAC GAGTCCATTT CTCCTACTTC CCCAACCGCC 
CTGGCTCCCC GTGTCCGCAG TCACTCCTTC TACGTGAGCC ACTCTTCCAA AGACAGGAGT 
GGGGCGGACC GTGGAGAGGG CTTCTTTAAA GACAAGTTGT TCAGCCTCCA CCGGGCCAAC 
AGCTCACAGT CTGTGTCCTC AGGAGCAGGT CCCAAAGAGT CCAAGCCAAC CCCTTTGAAT 
ACTTTATCCG TGCAGCAGCA GCTCCGCCCT TCTTCCTGCA TAGACATCTA TGTTTCTGCC 
TCAGAGGACG TCCCGCCCAC TGAGAGCTTC TTGGATTCGG TTCGGACAGT TCCAACTCTG 
GCTCGAGACT CATCTCTTCA TTCGGAGATC ATGGAGGCGG TGCTCTCAGG GGGACGGGAC 
TGTAGTGGAA GGGCAGGCGG CAGCGAAAGA CAGTCGGATG GTACAGTCCT GTTTGAGGAC 
AGCGCCGCCG CTGACCTATC GCTCTGCTCT GCCCACCTGC TGCCGGATTC CTTGCCCCCC 
TGGGACCTGG ACCCCTCACC GCCACCATCA GCTGGTGTAC TAGAGCGGTC CAAAAGTAGT 
CGCTTCCTGT CTACAGCAGG GCCTCTGTTT CTGGACGAGC CTCATTTGGT TAAGTCACAC 
AGTCTGATGT TCACATCACG TGGGTACTAT GGTGGCATGG GTGTGCAGGT GAAGGCGGCC 
GAATACACCA GCATCACAGA TTGCATCGAC ACCCGTTGCG TCTCAACCCC TTACCCTGTG 
CCAGAGCGGT CAGACTCACC TGGAGGATCT TTCACCTTCG ATAAGCCTCA AGACCTTGGC 
GTCTCGCACC CTGAGAGAGA CGCTGAGCTC AGCCACGCTG AGTCTGACTT AGAGGAGCCA 
GCAGAGGGTT CGGGAGACAC ATGTAAGGTG GGTCAGAGTA GCAGTAGTGG GGTAATAGGT 
GCAGACCTGG GCCTGGGCCT CGCACTAGGC CCGTTTTGCT CACCCATCTC TAGACTAGAG 
CGAGCAAACA GCTGCTCCTC TTCAGAGGAG TCGCACTCCA ACATCTACTC TCGAAAGAGC 
TTCTCAATAA GCGAGAGAAT GGACAAGGGC CGGGGGAGCT CCAGGAACCC TTTCCAGAAG 
GCCAGAACCG GGGCGAGGCT GGAGGGTAAG ACAGACTCAC TGTCTATGAG GAAAATGGCC 
AAACCGTCAG CTTTCAGAAG TTTTGACAGC AGACACAACT ACACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299833.1
CDS	338..5545
Translation	MTKRKWEGRREGFKVSSTREEAQGPGRPSTRGRFSESWKRLSSRQGSTKRSGLASQQAAAQKSWIERAFSKRECVHIIVSTKDHHRCCCGRLIGQHVGLPPSISSNQNEKSERVPKNDSLSEKWSISKHTQLSPTDAFGTIEFQGGGHSNKAMYVRVSYDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLVGKDVVRPYQTMSNPLSKLTVLNSLHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGIINESLRDQLLVTIQKTFTYSRTQAQHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQSMLDALVLDRVDFVKLLIENGVSMHRFLTLSRLEELYNTRHGPSNTLYHLVRDVKKGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDMPIRRGRQKTTRKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNQNSREFGQLAVELLDQSYKQDEQMAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGLLLPPSILSLEFKNKDEMSYMPQDQDTYLQEKDVDEPEKQAKEKEEEDMEFTVRSYCETQYNSVAMLGNVSSEASRKKQVEEVQNRHRLIPLGRKIYEFYNAPIVKFWFHTLAYVGYLMLFNYIVLVKMDLWPSPQEWIVIAYIFTNGIEKMREILMSEPGKLLQKVKVWLQEYWNITDLMAILIFSIGMVLRLQDPPLMSYGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDPPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHITMVLKHLCCRWRKHDEDERDYGLKLFITEDELKKVHDFEEQCMEEYFREKDDRFNSSNDERIRVTSERVENMAMRLEEVNEREHFMKASLQTVDIRLAQMEEMIGRIAVALERVAGMDRGEVNKARSRTSSDCTDTNYILRQSSFNSQEGNSYRLQESLEQGGDESISPTSPTALAPRVRSHSFYVSHSSKDRSGADRGEGFFKDKLFSLHRANSSQSVSSGAGPKESKPTPLNTLSVQQQLRPSSCIDIYVSASEDVPPTESFLDSVRTVPTLARDSSLHSEIMEAVLSGGRDCSGRAGGSERQSDGTVLFEDSAAADLSLCSAHLLPDSLPPWDLDPSPPPSAGVLERSKSSRFLSTAGPLFLDEPHLVKSHSLMFTSRGYYGGMGVQVKAAEYTSITDCIDTRCVSTPYPVPERSDSPGGSFTFDKPQDLGVSHPERDAELSHAESDLEEPAEGSGDTCKVGQSSSSGVIGADLGLGLALGPFCSPISRLERANSCSSSEESHSNIYSRKSFSISERMDKGRGSSRNPFQKARTGARLEGKTDSLSMRKMAKPSAFRSFDSRHNYT

Target ORF information:

RefSeq Version	XM_009301558.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301558.2

ORF Insert Sequence:

ATGACAAAAA GAAAATGGGA GGGGAGGAGA GAAGGCTTCA AGGTGAGCTC CACGAGGGAG 
GAGGCACAGG GGCCTGGACG CCCCTCCACC AGAGGGCGCT TCTCCGAATC TTGGAAGAGA 
CTGAGCTCCA GACAGGGGTC TACCAAGAGG TCAGGACTGG CCTCCCAGCA AGCTGCGGCA 
CAGAAATCAT GGATAGAAAG AGCGTTCAGC AAGAGAGAAT GTGTGCACAT TATTGTAAGC 
ACCAAAGACC ACCATAGGTG CTGTTGTGGC CGTCTAATTG GTCAACATGT TGGCCTGCCC 
CCCAGCATCT CATCCAATCA GAATGAGAAG TCAGAACGCG TGCCAAAGAA TGACAGCTTG 
TCAGAGAAGT GGTCCATCAG CAAGCACACC CAGCTCAGTC CCACCGACGC TTTTGGGACC 
ATCGAGTTCC AGGGAGGAGG ACACTCTAAT AAAGCAATGT ATGTACGAGT GTCTTACGAC 
ACAAAACCAG ATTTACTGCT GCATCTGATG ACAAAGGAGT GGCAACTGGA GCTTCCCAAG 
CTGCTCATCT CTGTTCACGG AGGACTTCAA AACTTTGAGT TGCAGCCCAA ACTAAAGCAA 
GTCTTCGGCA AAGGTCTTAT AAAGGCTGCA ATGACTACCG GAGCCTGGAT CTTCACTGGA 
GGAGTTAATA CTGGAGTCAT CAGACATGTT GGTGATGCTC TGAAAGATCA TGCTTCCAAA 
TCAAGAGGGA AGATCTGCAC TATCGGCATT GCCCCCTGGG GGATTGTGGA GAACCAGGAA 
GACCTGGTGG GCAAAGATGT GGTCCGGCCA TACCAGACGA TGTCTAACCC TCTTAGCAAG 
CTAACAGTAC TCAACAGCCT ACACTCACAC TTCATTTTGG CTGATAATGG CACCACCGGA 
AAGTACGGAG CAGAAGTCAA ACTGCGCAGG CAGCTGGAGA AGCACATCTC TCTGCAAAAG 
ATTAACACAC GGATTGGACA GGGTGTTCCA GTAGTGGCTC TGATAGTGGA GGGGGGTCCG 
AATGTCATCT CTATCGTCCT GGAGTACCTA AGAGACACCC CTCCGGTGCC TGTTGTGGTG 
TGTGACGGCA GCGGTCGAGC CTCTGACATC CTGGCCTTTG GGCACAAGTA CTCAGAGGAG 
GGCGGAATCA TAAACGAATC TTTGAGAGAC CAGTTATTAG TCACCATTCA GAAGACGTTC 
ACATACAGCC GGACGCAGGC CCAGCATCTC TTCATCATTC TGATGGAGTG CATGAAGAAG 
AAAGAACTGA TCACAGTGTT TCGGATGGGA TCAGAGGGAC ACCAGGACAT CGATCTAGCC 
ATTCTAACAG CTCTTCTGAA AGGAGCGAAT GCATCAGCTC CAGATCAGCT AAGTCTGGCT 
TTGGCCTGGA ACAGAGTTGA CATCGCCCGG AGCCAGATCT TCATTTACGG ACAGCAGTGG 
CCTGTTGGCT CTCTGGAGCA GTCTATGCTG GACGCGCTGG TGCTGGACCG AGTGGACTTT 
GTAAAGCTGT TGATTGAGAA CGGAGTGAGC ATGCACCGTT TCCTGACACT CTCCAGACTT 
GAAGAGCTTT ATAATACGAG GCACGGACCA TCCAACACCT TGTACCACCT TGTCAGAGAT 
GTGAAAAAGG GTAACCTGCC CCCCGACTAC CGCATTAGCC TGATTGACAT CGGATTGGTT 
ATTGAATACC TCATGGGTGG AGCCTATCGC TGCAATTACA CCAGGAAGAG ATTTCGGACC 
CTCTATCACA ACCTCTTTGG ACCCAAAAGA CCAAAAGCCC TGAAGCTGCT TGGAATGGAG 
GATGACATGC CCATTAGACG AGGGCGTCAG AAGACTACGC GGAAGCGAGA GGAGGAAGTA 
GATATTGACC TGGATGACCC AGAAATCAAT CATTTCCCTT TCCCATTCCA TGAGCTGATG 
GTTTGGGCTG TGCTGATGAA ACGGCAGAAG ATGGCACTGT TCTTCTGGCA GCATGGAGAG 
GAGGCCATGG CTAAAGCGCT GGTGGCCTGC AAGCTCTGTA AGGCAATGGC TCACGAAGCT 
TCTGAGAACG ACATGGTCGA TGACATTTCA CAGGAGCTCA ATCAAAACTC AAGGGAGTTT 
GGTCAGCTGG CTGTTGAGCT GCTCGATCAG TCCTATAAGC AGGATGAACA GATGGCTATG 
AAGCTGTTGA CCTACGAGCT GAAGAACTGG AGTAATGCCA CCTGTCTGCA GCTGGCAGTG 
GCTGCCAAGC ACCGAGACTT CATCGCCCAC ACCTGTAGCC AGATGCTGCT CACAGACATG 
TGGATGGGAC GTCTGCGCAT GCGCAAGAAC TCTGGCCTGA AGGTTATCTT GGGCCTGCTT 
CTCCCCCCTT CCATATTGAG TCTGGAGTTT AAAAATAAAG ACGAGATGTC ATACATGCCT 
CAGGATCAGG ACACATATCT GCAGGAGAAG GATGTGGATG AGCCCGAGAA ACAAGCTAAG 
GAGAAAGAGG AGGAGGACAT GGAGTTCACA GTAAGATCTT ACTGTGAGAC GCAGTACAAC 
TCCGTGGCTA TGTTGGGAAA CGTCAGTTCA GAAGCATCGC GCAAGAAACA GGTGGAGGAG 
GTTCAGAACC GGCATCGGCT CATTCCATTG GGTCGCAAAA TATATGAGTT TTACAACGCC 
CCTATCGTCA AGTTCTGGTT TCACACGCTG GCGTATGTAG GATACTTGAT GCTCTTCAAC 
TATATTGTGC TGGTGAAGAT GGACCTGTGG CCTTCTCCTC AGGAGTGGAT TGTTATTGCC 
TACATATTCA CCAATGGCAT CGAGAAGATG AGAGAGATCC TGATGTCCGA ACCTGGGAAG 
CTGCTTCAGA AGGTGAAAGT GTGGTTACAG GAGTACTGGA ACATCACTGA CCTCATGGCC 
ATCCTCATCT TCTCCATCGG GATGGTGCTT CGTCTGCAGG ACCCGCCGCT GATGAGCTAC 
GGCAGGGTCA TTTACTGCGT CAATATCATT TATTGGTACA TACGACTCCT CGACATCTTC 
GGAGTGAACA AATACCTGGG TCCATACGTC ATGATGATCG GCAAGATGAT GATTGACATG 
ATGTATTTTG TGATCATCAT GTTGGTGGTG CTGATGAGTT TCGGTGTGGC GCGTCAGGCC 
ATCCTTAATC CCAATGAAGA CCCATCCTGG ATGCTGGCAC GCAATATCTT CTTCATGCCA 
TACTGGATGA TCTACGGAGA GGTGTTTGCT GACCAAATCG ACCCTCCTTG TGGTCAGAAC 
ATCACCACAG AAGAAGGAGT TGTCATGCCT CTTCCACCCT GCAAAACTGG AGCTTGGATT 
GTCCCTGCCA TAATGGCCTG CTACCTGCTG GTGGCCAACA TCCTGCTAGT TAACTTGCTC 
ATTGCTGTCT TTAATAACAC TTTCTTTGAA GTCAAGTCCA TTTCCAACCA GGTGTGGAAG 
TTTCAGAGGT ATCAGCTGAT CATGACCTTC CATGAACGCC CGGTCCTTCC TCCTCCACTC 
ATCATCTTCA GCCACATAAC TATGGTTCTC AAACACCTGT GCTGTCGCTG GAGGAAGCAC 
GATGAGGATG AACGGGACTA TGGGCTGAAG CTTTTCATCA CTGAAGATGA ACTGAAGAAA 
GTGCATGACT TTGAAGAGCA GTGCATGGAG GAGTATTTCA GAGAGAAAGA TGACCGCTTC 
AATTCCTCCA ATGACGAAAG GATACGTGTG ACATCTGAAA GGGTGGAGAA CATGGCGATG 
CGATTAGAGG AGGTGAATGA GAGGGAACAT TTTATGAAAG CGTCCTTGCA GACTGTAGAC 
ATCCGGCTCG CCCAGATGGA GGAGATGATC GGCCGCATTG CTGTGGCCCT AGAGAGAGTT 
GCTGGAATGG ACAGAGGCGA AGTTAACAAA GCCCGGTCCA GGACATCATC AGACTGCACT 
GATACCAATT ACATCCTGCG CCAAAGCAGT TTCAACAGCC AAGAAGGCAA TTCCTACAGG 
CTACAGGAGT CTCTGGAGCA GGGGGGCGAC GAGTCCATTT CTCCTACTTC CCCAACCGCC 
CTGGCTCCCC GTGTCCGCAG TCACTCCTTC TACGTGAGCC ACTCTTCCAA AGACAGGAGT 
GGGGCGGACC GTGGAGAGGG CTTCTTTAAA GACAAGTTGT TCAGCCTCCA CCGGGCCAAC 
AGCTCACAGT CTGTGTCCTC AGGAGCAGGT CCCAAAGAGT CCAAGCCAAC CCCTTTGAAT 
ACTTTATCCG TGCAGCAGCA GCTCCGCCCT TCTTCCTGCA TAGACATCTA TGTTTCTGCC 
TCAGAGGACG TCCCGCCCAC TGAGAGCTTC TTGGATTCGG TTCGGACAGT TCCAACTCTG 
GCTCGAGACT CATCTCTTCA TTCGGAGATC ATGGAGGCGG TGCTCTCAGG GGGACGGGAC 
TGTAGTGGAA GGGCAGGCGG CAGCGAAAGA CAGTCGGATG GTACAGTCCT GTTTGAGGAC 
AGCGCCGCCG CTGACCTATC GCTCTGCTCT GCCCACCTGC TGCCGGATTC CTTGCCCCCC 
TGGGACCTGG ACCCCTCACC GCCACCATCA GCTGGTGTAC TAGAGCGGTC CAAAAGTAGT 
CGCTTCCTGT CTACAGCAGG GCCTCTGTTT CTGGACGAGC CTCATTTGGT TAAGTCACAC 
AGTCTGATGT TCACATCACG TGGGTACTAT GGTGGCATGG GTGTGCAGGT GAAGGCGGCC 
GAATACACCA GCATCACAGA TTGCATCGAC ACCCGTTGCG TCTCAACCCC TTACCCTGTG 
CCAGAGCGGT CAGACTCACC TGGAGGATCT TTCACCTTCG ATAAGCCTCA AGACCTTGGC 
GTCTCGCACC CTGAGAGAGA CGCTGAGCTC AGCCACGCTG AGTCTGACTT AGAGGAGCCA 
GCAGAGGGTT CGGGAGACAC ATGTAAGGTG GGTCAGAGTA GCAGTAGTGG GGTAATAGGT 
GCAGACCTGG GCCTGGGCCT CGCACTAGGC CCGTTTTGCT CACCCATCTC TAGACTAGAG 
CGAGCAAACA GCTGCTCCTC TTCAGAGGAG TCGCACTCCA ACATCTACTC TCGAAAGAGC 
TTCTCAATAA GCGAGAGAAT GGACAAGGGC CGGGGGAGCT CCAGGAACCC TTTCCAGAAG 
GCCAGAACCG GGGCGAGGCT GGAGGGTAAG ACAGACTCAC TGTCTATGAG GAAAATGGCC 
AAACCGTCAG CTTTCAGAAG TTTTGACAGC AGACACAACT ACACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm3 ( XM_009301558.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299833.1 Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X10, mRNA.

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CloneID	ODa44719
Clone ID Related Accession (Same CDS sequence)	XM_009301558.2 , XM_009301558.3
Accession Version	XM_009301558.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily M member 3 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301558.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAAAAA GAAAATGGGA GGGGAGGAGA GAAGGCTTCA AGGTGAGCTC CACGAGGGAG 
GAGGCACAGG GGCCTGGACG CCCCTCCACC AGAGGGCGCT TCTCCGAATC TTGGAAGAGA 
CTGAGCTCCA GACAGGGGTC TACCAAGAGG TCAGGACTGG CCTCCCAGCA AGCTGCGGCA 
CAGAAATCAT GGATAGAAAG AGCGTTCAGC AAGAGAGAAT GTGTGCACAT TATTGTAAGC 
ACCAAAGACC ACCATAGGTG CTGTTGTGGC CGTCTAATTG GTCAACATGT TGGCCTGCCC 
CCCAGCATCT CATCCAATCA GAATGAGAAG TCAGAACGCG TGCCAAAGAA TGACAGCTTG 
TCAGAGAAGT GGTCCATCAG CAAGCACACC CAGCTCAGTC CCACCGACGC TTTTGGGACC 
ATCGAGTTCC AGGGAGGAGG ACACTCTAAT AAAGCAATGT ATGTACGAGT GTCTTACGAC 
ACAAAACCAG ATTTACTGCT GCATCTGATG ACAAAGGAGT GGCAACTGGA GCTTCCCAAG 
CTGCTCATCT CTGTTCACGG AGGACTTCAA AACTTTGAGT TGCAGCCCAA ACTAAAGCAA 
GTCTTCGGCA AAGGTCTTAT AAAGGCTGCA ATGACTACCG GAGCCTGGAT CTTCACTGGA 
GGAGTTAATA CTGGAGTCAT CAGACATGTT GGTGATGCTC TGAAAGATCA TGCTTCCAAA 
TCAAGAGGGA AGATCTGCAC TATCGGCATT GCCCCCTGGG GGATTGTGGA GAACCAGGAA 
GACCTGGTGG GCAAAGATGT GGTCCGGCCA TACCAGACGA TGTCTAACCC TCTTAGCAAG 
CTAACAGTAC TCAACAGCCT ACACTCACAC TTCATTTTGG CTGATAATGG CACCACCGGA 
AAGTACGGAG CAGAAGTCAA ACTGCGCAGG CAGCTGGAGA AGCACATCTC TCTGCAAAAG 
ATTAACACAC GGATTGGACA GGGTGTTCCA GTAGTGGCTC TGATAGTGGA GGGGGGTCCG 
AATGTCATCT CTATCGTCCT GGAGTACCTA AGAGACACCC CTCCGGTGCC TGTTGTGGTG 
TGTGACGGCA GCGGTCGAGC CTCTGACATC CTGGCCTTTG GGCACAAGTA CTCAGAGGAG 
GGCGGAATCA TAAACGAATC TTTGAGAGAC CAGTTATTAG TCACCATTCA GAAGACGTTC 
ACATACAGCC GGACGCAGGC CCAGCATCTC TTCATCATTC TGATGGAGTG CATGAAGAAG 
AAAGAACTGA TCACAGTGTT TCGGATGGGA TCAGAGGGAC ACCAGGACAT CGATCTAGCC 
ATTCTAACAG CTCTTCTGAA AGGAGCGAAT GCATCAGCTC CAGATCAGCT AAGTCTGGCT 
TTGGCCTGGA ACAGAGTTGA CATCGCCCGG AGCCAGATCT TCATTTACGG ACAGCAGTGG 
CCTGTTGGCT CTCTGGAGCA GTCTATGCTG GACGCGCTGG TGCTGGACCG AGTGGACTTT 
GTAAAGCTGT TGATTGAGAA CGGAGTGAGC ATGCACCGTT TCCTGACACT CTCCAGACTT 
GAAGAGCTTT ATAATACGAG GCACGGACCA TCCAACACCT TGTACCACCT TGTCAGAGAT 
GTGAAAAAGG GTAACCTGCC CCCCGACTAC CGCATTAGCC TGATTGACAT CGGATTGGTT 
ATTGAATACC TCATGGGTGG AGCCTATCGC TGCAATTACA CCAGGAAGAG ATTTCGGACC 
CTCTATCACA ACCTCTTTGG ACCCAAAAGA CCAAAAGCCC TGAAGCTGCT TGGAATGGAG 
GATGACATGC CCATTAGACG AGGGCGTCAG AAGACTACGC GGAAGCGAGA GGAGGAAGTA 
GATATTGACC TGGATGACCC AGAAATCAAT CATTTCCCTT TCCCATTCCA TGAGCTGATG 
GTTTGGGCTG TGCTGATGAA ACGGCAGAAG ATGGCACTGT TCTTCTGGCA GCATGGAGAG 
GAGGCCATGG CTAAAGCGCT GGTGGCCTGC AAGCTCTGTA AGGCAATGGC TCACGAAGCT 
TCTGAGAACG ACATGGTCGA TGACATTTCA CAGGAGCTCA ATCAAAACTC AAGGGAGTTT 
GGTCAGCTGG CTGTTGAGCT GCTCGATCAG TCCTATAAGC AGGATGAACA GATGGCTATG 
AAGCTGTTGA CCTACGAGCT GAAGAACTGG AGTAATGCCA CCTGTCTGCA GCTGGCAGTG 
GCTGCCAAGC ACCGAGACTT CATCGCCCAC ACCTGTAGCC AGATGCTGCT CACAGACATG 
TGGATGGGAC GTCTGCGCAT GCGCAAGAAC TCTGGCCTGA AGGTTATCTT GGGCCTGCTT 
CTCCCCCCTT CCATATTGAG TCTGGAGTTT AAAAATAAAG ACGAGATGTC ATACATGCCT 
CAGGATCAGG ACACATATCT GCAGGAGAAG GATGTGGATG AGCCCGAGAA ACAAGCTAAG 
GAGAAAGAGG AGGAGGACAT GGAGTTCACA GTAAGATCTT ACTGTGAGAC GCAGTACAAC 
TCCGTGGCTA TGTTGGGAAA CGTCAGTTCA GAAGCATCGC GCAAGAAACA GGTGGAGGAG 
GTTCAGAACC GGCATCGGCT CATTCCATTG GGTCGCAAAA TATATGAGTT TTACAACGCC 
CCTATCGTCA AGTTCTGGTT TCACACGCTG GCGTATGTAG GATACTTGAT GCTCTTCAAC 
TATATTGTGC TGGTGAAGAT GGACCTGTGG CCTTCTCCTC AGGAGTGGAT TGTTATTGCC 
TACATATTCA CCAATGGCAT CGAGAAGATG AGAGAGATCC TGATGTCCGA ACCTGGGAAG 
CTGCTTCAGA AGGTGAAAGT GTGGTTACAG GAGTACTGGA ACATCACTGA CCTCATGGCC 
ATCCTCATCT TCTCCATCGG GATGGTGCTT CGTCTGCAGG ACCCGCCGCT GATGAGCTAC 
GGCAGGGTCA TTTACTGCGT CAATATCATT TATTGGTACA TACGACTCCT CGACATCTTC 
GGAGTGAACA AATACCTGGG TCCATACGTC ATGATGATCG GCAAGATGAT GATTGACATG 
ATGTATTTTG TGATCATCAT GTTGGTGGTG CTGATGAGTT TCGGTGTGGC GCGTCAGGCC 
ATCCTTAATC CCAATGAAGA CCCATCCTGG ATGCTGGCAC GCAATATCTT CTTCATGCCA 
TACTGGATGA TCTACGGAGA GGTGTTTGCT GACCAAATCG ACCCTCCTTG TGGTCAGAAC 
ATCACCACAG AAGAAGGAGT TGTCATGCCT CTTCCACCCT GCAAAACTGG AGCTTGGATT 
GTCCCTGCCA TAATGGCCTG CTACCTGCTG GTGGCCAACA TCCTGCTAGT TAACTTGCTC 
ATTGCTGTCT TTAATAACAC TTTCTTTGAA GTCAAGTCCA TTTCCAACCA GGTGTGGAAG 
TTTCAGAGGT ATCAGCTGAT CATGACCTTC CATGAACGCC CGGTCCTTCC TCCTCCACTC 
ATCATCTTCA GCCACATAAC TATGGTTCTC AAACACCTGT GCTGTCGCTG GAGGAAGCAC 
GATGAGGATG AACGGGACTA TGGGCTGAAG CTTTTCATCA CTGAAGATGA ACTGAAGAAA 
GTGCATGACT TTGAAGAGCA GTGCATGGAG GAGTATTTCA GAGAGAAAGA TGACCGCTTC 
AATTCCTCCA ATGACGAAAG GATACGTGTG ACATCTGAAA GGGTGGAGAA CATGGCGATG 
CGATTAGAGG AGGTGAATGA GAGGGAACAT TTTATGAAAG CGTCCTTGCA GACTGTAGAC 
ATCCGGCTCG CCCAGATGGA GGAGATGATC GGCCGCATTG CTGTGGCCCT AGAGAGAGTT 
GCTGGAATGG ACAGAGGCGA AGTTAACAAA GCCCGGTCCA GGACATCATC AGACTGCACT 
GATACCAATT ACATCCTGCG CCAAAGCAGT TTCAACAGCC AAGAAGGCAA TTCCTACAGG 
CTACAGGAGT CTCTGGAGCA GGGGGGCGAC GAGTCCATTT CTCCTACTTC CCCAACCGCC 
CTGGCTCCCC GTGTCCGCAG TCACTCCTTC TACGTGAGCC ACTCTTCCAA AGACAGGAGT 
GGGGCGGACC GTGGAGAGGG CTTCTTTAAA GACAAGTTGT TCAGCCTCCA CCGGGCCAAC 
AGCTCACAGT CTGTGTCCTC AGGAGCAGGT CCCAAAGAGT CCAAGCCAAC CCCTTTGAAT 
ACTTTATCCG TGCAGCAGCA GCTCCGCCCT TCTTCCTGCA TAGACATCTA TGTTTCTGCC 
TCAGAGGACG TCCCGCCCAC TGAGAGCTTC TTGGATTCGG TTCGGACAGT TCCAACTCTG 
GCTCGAGACT CATCTCTTCA TTCGGAGATC ATGGAGGCGG TGCTCTCAGG GGGACGGGAC 
TGTAGTGGAA GGGCAGGCGG CAGCGAAAGA CAGTCGGATG GTACAGTCCT GTTTGAGGAC 
AGCGCCGCCG CTGACCTATC GCTCTGCTCT GCCCACCTGC TGCCGGATTC CTTGCCCCCC 
TGGGACCTGG ACCCCTCACC GCCACCATCA GCTGGTGTAC TAGAGCGGTC CAAAAGTAGT 
CGCTTCCTGT CTACAGCAGG GCCTCTGTTT CTGGACGAGC CTCATTTGGT TAAGTCACAC 
AGTCTGATGT TCACATCACG TGGGTACTAT GGTGGCATGG GTGTGCAGGT GAAGGCGGCC 
GAATACACCA GCATCACAGA TTGCATCGAC ACCCGTTGCG TCTCAACCCC TTACCCTGTG 
CCAGAGCGGT CAGACTCACC TGGAGGATCT TTCACCTTCG ATAAGCCTCA AGACCTTGGC 
GTCTCGCACC CTGAGAGAGA CGCTGAGCTC AGCCACGCTG AGTCTGACTT AGAGGAGCCA 
GCAGAGGGTT CGGGAGACAC ATGTAAGGTG GGTCAGAGTA GCAGTAGTGG GGTAATAGGT 
GCAGACCTGG GCCTGGGCCT CGCACTAGGC CCGTTTTGCT CACCCATCTC TAGACTAGAG 
CGAGCAAACA GCTGCTCCTC TTCAGAGGAG TCGCACTCCA ACATCTACTC TCGAAAGAGC 
TTCTCAATAA GCGAGAGAAT GGACAAGGGC CGGGGGAGCT CCAGGAACCC TTTCCAGAAG 
GCCAGAACCG GGGCGAGGCT GGAGGGTAAG ACAGACTCAC TGTCTATGAG GAAAATGGCC 
AAACCGTCAG CTTTCAGAAG TTTTGACAGC AGACACAACT ACACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299833.1
CDS	378..5585
Translation	MTKRKWEGRREGFKVSSTREEAQGPGRPSTRGRFSESWKRLSSRQGSTKRSGLASQQAAAQKSWIERAFSKRECVHIIVSTKDHHRCCCGRLIGQHVGLPPSISSNQNEKSERVPKNDSLSEKWSISKHTQLSPTDAFGTIEFQGGGHSNKAMYVRVSYDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLVGKDVVRPYQTMSNPLSKLTVLNSLHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGIINESLRDQLLVTIQKTFTYSRTQAQHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQSMLDALVLDRVDFVKLLIENGVSMHRFLTLSRLEELYNTRHGPSNTLYHLVRDVKKGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDMPIRRGRQKTTRKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNQNSREFGQLAVELLDQSYKQDEQMAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGLLLPPSILSLEFKNKDEMSYMPQDQDTYLQEKDVDEPEKQAKEKEEEDMEFTVRSYCETQYNSVAMLGNVSSEASRKKQVEEVQNRHRLIPLGRKIYEFYNAPIVKFWFHTLAYVGYLMLFNYIVLVKMDLWPSPQEWIVIAYIFTNGIEKMREILMSEPGKLLQKVKVWLQEYWNITDLMAILIFSIGMVLRLQDPPLMSYGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDPPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHITMVLKHLCCRWRKHDEDERDYGLKLFITEDELKKVHDFEEQCMEEYFREKDDRFNSSNDERIRVTSERVENMAMRLEEVNEREHFMKASLQTVDIRLAQMEEMIGRIAVALERVAGMDRGEVNKARSRTSSDCTDTNYILRQSSFNSQEGNSYRLQESLEQGGDESISPTSPTALAPRVRSHSFYVSHSSKDRSGADRGEGFFKDKLFSLHRANSSQSVSSGAGPKESKPTPLNTLSVQQQLRPSSCIDIYVSASEDVPPTESFLDSVRTVPTLARDSSLHSEIMEAVLSGGRDCSGRAGGSERQSDGTVLFEDSAAADLSLCSAHLLPDSLPPWDLDPSPPPSAGVLERSKSSRFLSTAGPLFLDEPHLVKSHSLMFTSRGYYGGMGVQVKAAEYTSITDCIDTRCVSTPYPVPERSDSPGGSFTFDKPQDLGVSHPERDAELSHAESDLEEPAEGSGDTCKVGQSSSSGVIGADLGLGLALGPFCSPISRLERANSCSSSEESHSNIYSRKSFSISERMDKGRGSSRNPFQKARTGARLEGKTDSLSMRKMAKPSAFRSFDSRHNYT

Target ORF information:

RefSeq Version	XM_009301558.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301558.3

ORF Insert Sequence:

ATGACAAAAA GAAAATGGGA GGGGAGGAGA GAAGGCTTCA AGGTGAGCTC CACGAGGGAG 
GAGGCACAGG GGCCTGGACG CCCCTCCACC AGAGGGCGCT TCTCCGAATC TTGGAAGAGA 
CTGAGCTCCA GACAGGGGTC TACCAAGAGG TCAGGACTGG CCTCCCAGCA AGCTGCGGCA 
CAGAAATCAT GGATAGAAAG AGCGTTCAGC AAGAGAGAAT GTGTGCACAT TATTGTAAGC 
ACCAAAGACC ACCATAGGTG CTGTTGTGGC CGTCTAATTG GTCAACATGT TGGCCTGCCC 
CCCAGCATCT CATCCAATCA GAATGAGAAG TCAGAACGCG TGCCAAAGAA TGACAGCTTG 
TCAGAGAAGT GGTCCATCAG CAAGCACACC CAGCTCAGTC CCACCGACGC TTTTGGGACC 
ATCGAGTTCC AGGGAGGAGG ACACTCTAAT AAAGCAATGT ATGTACGAGT GTCTTACGAC 
ACAAAACCAG ATTTACTGCT GCATCTGATG ACAAAGGAGT GGCAACTGGA GCTTCCCAAG 
CTGCTCATCT CTGTTCACGG AGGACTTCAA AACTTTGAGT TGCAGCCCAA ACTAAAGCAA 
GTCTTCGGCA AAGGTCTTAT AAAGGCTGCA ATGACTACCG GAGCCTGGAT CTTCACTGGA 
GGAGTTAATA CTGGAGTCAT CAGACATGTT GGTGATGCTC TGAAAGATCA TGCTTCCAAA 
TCAAGAGGGA AGATCTGCAC TATCGGCATT GCCCCCTGGG GGATTGTGGA GAACCAGGAA 
GACCTGGTGG GCAAAGATGT GGTCCGGCCA TACCAGACGA TGTCTAACCC TCTTAGCAAG 
CTAACAGTAC TCAACAGCCT ACACTCACAC TTCATTTTGG CTGATAATGG CACCACCGGA 
AAGTACGGAG CAGAAGTCAA ACTGCGCAGG CAGCTGGAGA AGCACATCTC TCTGCAAAAG 
ATTAACACAC GGATTGGACA GGGTGTTCCA GTAGTGGCTC TGATAGTGGA GGGGGGTCCG 
AATGTCATCT CTATCGTCCT GGAGTACCTA AGAGACACCC CTCCGGTGCC TGTTGTGGTG 
TGTGACGGCA GCGGTCGAGC CTCTGACATC CTGGCCTTTG GGCACAAGTA CTCAGAGGAG 
GGCGGAATCA TAAACGAATC TTTGAGAGAC CAGTTATTAG TCACCATTCA GAAGACGTTC 
ACATACAGCC GGACGCAGGC CCAGCATCTC TTCATCATTC TGATGGAGTG CATGAAGAAG 
AAAGAACTGA TCACAGTGTT TCGGATGGGA TCAGAGGGAC ACCAGGACAT CGATCTAGCC 
ATTCTAACAG CTCTTCTGAA AGGAGCGAAT GCATCAGCTC CAGATCAGCT AAGTCTGGCT 
TTGGCCTGGA ACAGAGTTGA CATCGCCCGG AGCCAGATCT TCATTTACGG ACAGCAGTGG 
CCTGTTGGCT CTCTGGAGCA GTCTATGCTG GACGCGCTGG TGCTGGACCG AGTGGACTTT 
GTAAAGCTGT TGATTGAGAA CGGAGTGAGC ATGCACCGTT TCCTGACACT CTCCAGACTT 
GAAGAGCTTT ATAATACGAG GCACGGACCA TCCAACACCT TGTACCACCT TGTCAGAGAT 
GTGAAAAAGG GTAACCTGCC CCCCGACTAC CGCATTAGCC TGATTGACAT CGGATTGGTT 
ATTGAATACC TCATGGGTGG AGCCTATCGC TGCAATTACA CCAGGAAGAG ATTTCGGACC 
CTCTATCACA ACCTCTTTGG ACCCAAAAGA CCAAAAGCCC TGAAGCTGCT TGGAATGGAG 
GATGACATGC CCATTAGACG AGGGCGTCAG AAGACTACGC GGAAGCGAGA GGAGGAAGTA 
GATATTGACC TGGATGACCC AGAAATCAAT CATTTCCCTT TCCCATTCCA TGAGCTGATG 
GTTTGGGCTG TGCTGATGAA ACGGCAGAAG ATGGCACTGT TCTTCTGGCA GCATGGAGAG 
GAGGCCATGG CTAAAGCGCT GGTGGCCTGC AAGCTCTGTA AGGCAATGGC TCACGAAGCT 
TCTGAGAACG ACATGGTCGA TGACATTTCA CAGGAGCTCA ATCAAAACTC AAGGGAGTTT 
GGTCAGCTGG CTGTTGAGCT GCTCGATCAG TCCTATAAGC AGGATGAACA GATGGCTATG 
AAGCTGTTGA CCTACGAGCT GAAGAACTGG AGTAATGCCA CCTGTCTGCA GCTGGCAGTG 
GCTGCCAAGC ACCGAGACTT CATCGCCCAC ACCTGTAGCC AGATGCTGCT CACAGACATG 
TGGATGGGAC GTCTGCGCAT GCGCAAGAAC TCTGGCCTGA AGGTTATCTT GGGCCTGCTT 
CTCCCCCCTT CCATATTGAG TCTGGAGTTT AAAAATAAAG ACGAGATGTC ATACATGCCT 
CAGGATCAGG ACACATATCT GCAGGAGAAG GATGTGGATG AGCCCGAGAA ACAAGCTAAG 
GAGAAAGAGG AGGAGGACAT GGAGTTCACA GTAAGATCTT ACTGTGAGAC GCAGTACAAC 
TCCGTGGCTA TGTTGGGAAA CGTCAGTTCA GAAGCATCGC GCAAGAAACA GGTGGAGGAG 
GTTCAGAACC GGCATCGGCT CATTCCATTG GGTCGCAAAA TATATGAGTT TTACAACGCC 
CCTATCGTCA AGTTCTGGTT TCACACGCTG GCGTATGTAG GATACTTGAT GCTCTTCAAC 
TATATTGTGC TGGTGAAGAT GGACCTGTGG CCTTCTCCTC AGGAGTGGAT TGTTATTGCC 
TACATATTCA CCAATGGCAT CGAGAAGATG AGAGAGATCC TGATGTCCGA ACCTGGGAAG 
CTGCTTCAGA AGGTGAAAGT GTGGTTACAG GAGTACTGGA ACATCACTGA CCTCATGGCC 
ATCCTCATCT TCTCCATCGG GATGGTGCTT CGTCTGCAGG ACCCGCCGCT GATGAGCTAC 
GGCAGGGTCA TTTACTGCGT CAATATCATT TATTGGTACA TACGACTCCT CGACATCTTC 
GGAGTGAACA AATACCTGGG TCCATACGTC ATGATGATCG GCAAGATGAT GATTGACATG 
ATGTATTTTG TGATCATCAT GTTGGTGGTG CTGATGAGTT TCGGTGTGGC GCGTCAGGCC 
ATCCTTAATC CCAATGAAGA CCCATCCTGG ATGCTGGCAC GCAATATCTT CTTCATGCCA 
TACTGGATGA TCTACGGAGA GGTGTTTGCT GACCAAATCG ACCCTCCTTG TGGTCAGAAC 
ATCACCACAG AAGAAGGAGT TGTCATGCCT CTTCCACCCT GCAAAACTGG AGCTTGGATT 
GTCCCTGCCA TAATGGCCTG CTACCTGCTG GTGGCCAACA TCCTGCTAGT TAACTTGCTC 
ATTGCTGTCT TTAATAACAC TTTCTTTGAA GTCAAGTCCA TTTCCAACCA GGTGTGGAAG 
TTTCAGAGGT ATCAGCTGAT CATGACCTTC CATGAACGCC CGGTCCTTCC TCCTCCACTC 
ATCATCTTCA GCCACATAAC TATGGTTCTC AAACACCTGT GCTGTCGCTG GAGGAAGCAC 
GATGAGGATG AACGGGACTA TGGGCTGAAG CTTTTCATCA CTGAAGATGA ACTGAAGAAA 
GTGCATGACT TTGAAGAGCA GTGCATGGAG GAGTATTTCA GAGAGAAAGA TGACCGCTTC 
AATTCCTCCA ATGACGAAAG GATACGTGTG ACATCTGAAA GGGTGGAGAA CATGGCGATG 
CGATTAGAGG AGGTGAATGA GAGGGAACAT TTTATGAAAG CGTCCTTGCA GACTGTAGAC 
ATCCGGCTCG CCCAGATGGA GGAGATGATC GGCCGCATTG CTGTGGCCCT AGAGAGAGTT 
GCTGGAATGG ACAGAGGCGA AGTTAACAAA GCCCGGTCCA GGACATCATC AGACTGCACT 
GATACCAATT ACATCCTGCG CCAAAGCAGT TTCAACAGCC AAGAAGGCAA TTCCTACAGG 
CTACAGGAGT CTCTGGAGCA GGGGGGCGAC GAGTCCATTT CTCCTACTTC CCCAACCGCC 
CTGGCTCCCC GTGTCCGCAG TCACTCCTTC TACGTGAGCC ACTCTTCCAA AGACAGGAGT 
GGGGCGGACC GTGGAGAGGG CTTCTTTAAA GACAAGTTGT TCAGCCTCCA CCGGGCCAAC 
AGCTCACAGT CTGTGTCCTC AGGAGCAGGT CCCAAAGAGT CCAAGCCAAC CCCTTTGAAT 
ACTTTATCCG TGCAGCAGCA GCTCCGCCCT TCTTCCTGCA TAGACATCTA TGTTTCTGCC 
TCAGAGGACG TCCCGCCCAC TGAGAGCTTC TTGGATTCGG TTCGGACAGT TCCAACTCTG 
GCTCGAGACT CATCTCTTCA TTCGGAGATC ATGGAGGCGG TGCTCTCAGG GGGACGGGAC 
TGTAGTGGAA GGGCAGGCGG CAGCGAAAGA CAGTCGGATG GTACAGTCCT GTTTGAGGAC 
AGCGCCGCCG CTGACCTATC GCTCTGCTCT GCCCACCTGC TGCCGGATTC CTTGCCCCCC 
TGGGACCTGG ACCCCTCACC GCCACCATCA GCTGGTGTAC TAGAGCGGTC CAAAAGTAGT 
CGCTTCCTGT CTACAGCAGG GCCTCTGTTT CTGGACGAGC CTCATTTGGT TAAGTCACAC 
AGTCTGATGT TCACATCACG TGGGTACTAT GGTGGCATGG GTGTGCAGGT GAAGGCGGCC 
GAATACACCA GCATCACAGA TTGCATCGAC ACCCGTTGCG TCTCAACCCC TTACCCTGTG 
CCAGAGCGGT CAGACTCACC TGGAGGATCT TTCACCTTCG ATAAGCCTCA AGACCTTGGC 
GTCTCGCACC CTGAGAGAGA CGCTGAGCTC AGCCACGCTG AGTCTGACTT AGAGGAGCCA 
GCAGAGGGTT CGGGAGACAC ATGTAAGGTG GGTCAGAGTA GCAGTAGTGG GGTAATAGGT 
GCAGACCTGG GCCTGGGCCT CGCACTAGGC CCGTTTTGCT CACCCATCTC TAGACTAGAG 
CGAGCAAACA GCTGCTCCTC TTCAGAGGAG TCGCACTCCA ACATCTACTC TCGAAAGAGC 
TTCTCAATAA GCGAGAGAAT GGACAAGGGC CGGGGGAGCT CCAGGAACCC TTTCCAGAAG 
GCCAGAACCG GGGCGAGGCT GGAGGGTAAG ACAGACTCAC TGTCTATGAG GAAAATGGCC 
AAACCGTCAG CTTTCAGAAG TTTTGACAGC AGACACAACT ACACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm3 ( XM_009301560.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299835.1 Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X4, mRNA.

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CloneID	ODa44720
Clone ID Related Accession (Same CDS sequence)	XM_009301560.2
Accession Version	XM_009301560.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transient receptor potential cation channel subfamily M member 3 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301560.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGGCC CTTTTCCGGA TGGCGGGAAC GTCCGGCCGG GAGCGATGAA CCAGCTGGAT 
GCTCCTCGGC CACTCAACTG GACCATACGG AAACTGTGCC ATGCAGCCTT CCTCCCGTCT 
GTGCGCCTGC TTAAGGCACA GAAATCATGG ATAGAAAGAG CGTTCAGCAA GAGAGAATGT 
GTGCACATTA TTGTAAGCAC CAAAGACCAC CATAGGTGCT GTTGTGGCCG TCTAATTGGT 
CAACATGTTG GCCTGCCCCC CAGCATCTCA TCCAATCAGA ATGAGAAGTC AGAACGCGTG 
CCAAAGAATG ACAGCTTGTC AGAGAAGTGG TCCATCAGCA AGCACACCCA GCTCAGTCCC 
ACCGACGCTT TTGGGACCAT CGAGTTCCAG GGAGGAGGAC ACTCTAATAA AGCAATGTAT 
GTACGAGTGT CTTACGACAC AAAACCAGAT TTACTGCTGC ATCTGATGAC AAAGGAGTGG 
CAACTGGAGC TTCCCAAGCT GCTCATCTCT GTTCACGGAG GACTTCAAAA CTTTGAGTTG 
CAGCCCAAAC TAAAGCAAGT CTTCGGCAAA GGTCTTATAA AGGCTGCAAT GACTACCGGA 
GCCTGGATCT TCACTGGAGG AGTTAATACT GGAGTCATCA GACATGTTGG TGATGCTCTG 
AAAGATCATG CTTCCAAATC AAGAGGGAAG ATCTGCACTA TCGGCATTGC CCCCTGGGGG 
ATTGTGGAGA ACCAGGAAGA CCTGGTGGGC AAAGATGTGG TCCGGCCATA CCAGACGATG 
TCTAACCCTC TTAGCAAGCT AACAGTACTC AACAGCCTAC ACTCACACTT CATTTTGGCT 
GATAATGGCA CCACCGGAAA GTACGGAGCA GAAGTCAAAC TGCGCAGGCA GCTGGAGAAG 
CACATCTCTC TGCAAAAGAT TAACACACGG ATTGGACAGG GTGTTCCAGT AGTGGCTCTG 
ATAGTGGAGG GGGGTCCGAA TGTCATCTCT ATCGTCCTGG AGTACCTAAG AGACACCCCT 
CCGGTGCCTG TTGTGGTGTG TGACGGCAGC GGTCGAGCCT CTGACATCCT GGCCTTTGGG 
CACAAGTACT CAGAGGAGGG CGGAATCATA AACGAATCTT TGAGAGACCA GTTATTAGTC 
ACCATTCAGA AGACGTTCAC ATACAGCCGG ACGCAGGCCC AGCATCTCTT CATCATTCTG 
ATGGAGTGCA TGAAGAAGAA AGAACTGATC ACAGTGTTTC GGATGGGATC AGAGGGACAC 
CAGGACATCG ATCTAGCCAT TCTAACAGCT CTTCTGAAAG GAGCGAATGC ATCAGCTCCA 
GATCAGCTAA GTCTGGCTTT GGCCTGGAAC AGAGTTGACA TCGCCCGGAG CCAGATCTTC 
ATTTACGGAC AGCAGTGGCC TGTTGGCTCT CTGGAGCAGT CTATGCTGGA CGCGCTGGTG 
CTGGACCGAG TGGACTTTGT AAAGCTGTTG ATTGAGAACG GAGTGAGCAT GCACCGTTTC 
CTGACACTCT CCAGACTTGA AGAGCTTTAT AATACGAGGC ACGGACCATC CAACACCTTG 
TACCACCTTG TCAGAGATGT GAAAAAGCAA GAATATCCAG GGTTCAGTTG GATCTACCTC 
AAGGGTAACC TGCCCCCCGA CTACCGCATT AGCCTGATTG ACATCGGATT GGTTATTGAA 
TACCTCATGG GTGGAGCCTA TCGCTGCAAT TACACCAGGA AGAGATTTCG GACCCTCTAT 
CACAACCTCT TTGGACCCAA AAGACCAAAA GCCCTGAAGC TGCTTGGAAT GGAGGATGAC 
ATGCCCATTA GACGAGGGCG TCAGAAGACT ACGCGGAAGC GAGAGGAGGA AGTAGATATT 
GACCTGGATG ACCCAGAAAT CAATCATTTC CCTTTCCCAT TCCATGAGCT GATGGTTTGG 
GCTGTGCTGA TGAAACGGCA GAAGATGGCA CTGTTCTTCT GGCAGCATGG AGAGGAGGCC 
ATGGCTAAAG CGCTGGTGGC CTGCAAGCTC TGTAAGGCAA TGGCTCACGA AGCTTCTGAG 
AACGACATGG TCGATGACAT TTCACAGGAG CTCAATCAAA ACTCAAGGGA GTTTGGTCAG 
CTGGCTGTTG AGCTGCTCGA TCAGTCCTAT AAGCAGGATG AACAGATGGC TATGAAGCTG 
TTGACCTACG AGCTGAAGAA CTGGAGTAAT GCCACCTGTC TGCAGCTGGC AGTGGCTGCC 
AAGCACCGAG ACTTCATCGC CCACACCTGT AGCCAGATGC TGCTCACAGA CATGTGGATG 
GGACGTCTGC GCATGCGCAA GAACTCTGGC CTGAAGGTTA TCTTGGGCCT GCTTCTCCCC 
CCTTCCATAT TGAGTCTGGA GTTTAAAAAT AAAGACGAGA TGTCATACAT GCCTCAGGAT 
CAGGACACAT ATCTGCAGGA GAAGGATGTG GATGAGCCCG AGAAACAAGC TAAGGAGAAA 
GAGGAGGAGG ACATGGAGTT CACAGTAAGA TCTTACTGTG AGACGCAGTA CAACTCCGTG 
GCTATGTTGG GAAACGTCAG TTCAGAAGCA TCGCGCAAGA AACAGGTGGA GGAGGTTCAG 
AACCGGCATC GGCTCATTCC ATTGGGTCGC AAAATATATG AGTTTTACAA CGCCCCTATC 
GTCAAGTTCT GGTTTCACAC GCTGGCGTAT GTAGGATACT TGATGCTCTT CAACTATATT 
GTGCTGGTGA AGATGGACCT GTGGCCTTCT CCTCAGGAGT GGATTGTTAT TGCCTACATA 
TTCACCAATG GCATCGAGAA GATGAGAGAG ATCCTGATGT CCGAACCTGG GAAGCTGCTT 
CAGAAGGTGA AAGTGTGGTT ACAGGAGTAC TGGAACATCA CTGACCTCAT GGCCATCCTC 
ATCTTCTCCA TCGGGATGGT GCTTCGTCTG CAGGACCCGC CGCTGATGAG CTACGGCAGG 
GTCATTTACT GCGTCAATAT CATTTATTGG TACATACGAC TCCTCGACAT CTTCGGAGTG 
AACAAATACC TGGGTCCATA CGTCATGATG ATCGGCAAGA TGATGATTGA CATGATGTAT 
TTTGTGATCA TCATGTTGGT GGTGCTGATG AGTTTCGGTG TGGCGCGTCA GGCCATCCTT 
AATCCCAATG AAGACCCATC CTGGATGCTG GCACGCAATA TCTTCTTCAT GCCATACTGG 
ATGATCTACG GAGAGGTGTT TGCTGACCAA ATCGACCCTC CTTGTGGTCA GAACATCACC 
ACAGAAGAAG GAGTTGTCAT GCCTCTTCCA CCCTGCAAAA CTGGAGCTTG GATTGTCCCT 
GCCATAATGG CCTGCTACCT GCTGGTGGCC AACATCCTGC TAGTTAACTT GCTCATTGCT 
GTCTTTAATA ACACTTTCTT TGAAGTCAAG TCCATTTCCA ACCAGGTGTG GAAGTTTCAG 
AGGTATCAGC TGATCATGAC CTTCCATGAA CGCCCGGTCC TTCCTCCTCC ACTCATCATC 
TTCAGCCACA TAACTATGGT TCTCAAACAC CTGTGCTGTC GCTGGAGGAA GCACGATGAG 
GATGAACGGG ACTATGGGCT GAAGCTTTTC ATCACTGAAG ATGAACTGAA GAAAGTGCAT 
GACTTTGAAG AGCAGTGCAT GGAGGAGTAT TTCAGAGAGA AAGATGACCG CTTCAATTCC 
TCCAATGACG AAAGGATACG TGTGACATCT GAAAGGGTGG AGAACATGGC GATGCGATTA 
GAGGAGGTGA ATGAGAGGGA ACATTTTATG AAAGCGTCCT TGCAGACTGT AGACATCCGG 
CTCGCCCAGA TGGAGGAGAT GATCGGCCGC ATTGCTGTGG CCCTAGAGAG AGTTGCTGGA 
ATGGACAGAG GCGAAGTTAA CAAAGCCCGG TCCAGGACAT CATCAGACTG CACTGATACC 
AATTACATCC TGCGCCAAAG CAGTTTCAAC AGCCAAGAAG GCAATTCCTA CAGGCTACAG 
GAGTCTCTGG AGCAGGGGGG CGACGAGTCC ATTTCTCCTA CTTCCCCAAC CGCCCTGGCT 
CCCCGTGTCC GCAGTCACTC CTTCTACGTG AGCCACTCTT CCAAAGACAG GAGTGGGGCG 
GACCGTGGAG AGGGCTTCTT TAAAGACAAG TTGTTCAGCC TCCACCGGGC CAACAGCTCA 
CAGTCTGTGT CCTCAGGAGC AGGTCCCAAA GAGTCCAAGC CAACCCCTTT GAATACTTTA 
TCCGTGCAGC AGCAGCTCCG CCCTTCTTCC TGCATAGACA TCTATGTTTC TGCCTCAGAG 
GACGTCCCGC CCACTGAGAG CTTCTTGGAT TCGGTTCGGA CAGTTCCAAC TCTGGCTCGA 
GACTCATCTC TTCATTCGGA GATCATGGAG GCGGTGCTCT CAGGGGGACG GGACTGTAGT 
GGAAGGGCAG GCGGCAGCGA AAGACAGTCG GATGGTACAG TCCTGTTTGA GGACAGCGCC 
GCCGCTGACC TATCGCTCTG CTCTGCCCAC CTGCTGCCGG ATTCCTTGCC CCCCTGGGAC 
CTGGACCCCT CACCGCCACC ATCAGCTGGT GTACTAGAGC GGTCCAAAAG TAGTCGCTTC 
CTGTCTACAG CAGGGCCTCT GTTTCTGGAC GAGCCTCATT TGGTTAAGTC ACACAGTCTG 
ATGTTCACAT CACGTGGGTA CTATGGTGGC ATGGGTGTGC AGGTGAAGGC GGCCGAATAC 
ACCAGCATCA CAGATTGCAT CGACACCCGT TGCGTCTCAA CCCCTTACCC TGTGCCAGAG 
CGGTCAGACT CACCTGGAGG ATCTTTCACC TTCGATAAGC CTCAAGACCT TGGCGTCTCG 
CACCCTGAGA GAGACGCTGA GCTCAGCCAC GCTGAGTCTG ACTTAGAGGA GCCAGCAGAG 
GGTTCGGGAG ACACATGTAA GGTGGGTCAG AGTAGCAGTA GTGGGGTAAT AGGTGCAGAC 
CTGGGCCTGG GCCTCGCACT AGGCCCGTTT TGCTCACCCA TCTCTAGACT AGAGCGAGCA 
AACAGCTGCT CCTCTTCAGA GGAGTCGCAC TCCAACATCT ACTCTCGAAA GAGCTTCTCA 
ATAAGCGAGA GAATGGACAA GGGCCGGGGG AGCTCCAGGA ACCCTTTCCA GAAGGCCAGA 
ACCGGGGCGA GGCTGGAGGG TAAGACAGAC TCACTGTCTA TGAGGAAAAT GGCCAAACCG 
TCAGCTTTCA GAAGTTTTGA CAGCAGACAC AACTACACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299835.1
CDS	375..5576
Translation	MWGPFPDGGNVRPGAMNQLDAPRPLNWTIRKLCHAAFLPSVRLLKAQKSWIERAFSKRECVHIIVSTKDHHRCCCGRLIGQHVGLPPSISSNQNEKSERVPKNDSLSEKWSISKHTQLSPTDAFGTIEFQGGGHSNKAMYVRVSYDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLVGKDVVRPYQTMSNPLSKLTVLNSLHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGIINESLRDQLLVTIQKTFTYSRTQAQHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQSMLDALVLDRVDFVKLLIENGVSMHRFLTLSRLEELYNTRHGPSNTLYHLVRDVKKQEYPGFSWIYLKGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDMPIRRGRQKTTRKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNQNSREFGQLAVELLDQSYKQDEQMAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGLLLPPSILSLEFKNKDEMSYMPQDQDTYLQEKDVDEPEKQAKEKEEEDMEFTVRSYCETQYNSVAMLGNVSSEASRKKQVEEVQNRHRLIPLGRKIYEFYNAPIVKFWFHTLAYVGYLMLFNYIVLVKMDLWPSPQEWIVIAYIFTNGIEKMREILMSEPGKLLQKVKVWLQEYWNITDLMAILIFSIGMVLRLQDPPLMSYGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDPPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHITMVLKHLCCRWRKHDEDERDYGLKLFITEDELKKVHDFEEQCMEEYFREKDDRFNSSNDERIRVTSERVENMAMRLEEVNEREHFMKASLQTVDIRLAQMEEMIGRIAVALERVAGMDRGEVNKARSRTSSDCTDTNYILRQSSFNSQEGNSYRLQESLEQGGDESISPTSPTALAPRVRSHSFYVSHSSKDRSGADRGEGFFKDKLFSLHRANSSQSVSSGAGPKESKPTPLNTLSVQQQLRPSSCIDIYVSASEDVPPTESFLDSVRTVPTLARDSSLHSEIMEAVLSGGRDCSGRAGGSERQSDGTVLFEDSAAADLSLCSAHLLPDSLPPWDLDPSPPPSAGVLERSKSSRFLSTAGPLFLDEPHLVKSHSLMFTSRGYYGGMGVQVKAAEYTSITDCIDTRCVSTPYPVPERSDSPGGSFTFDKPQDLGVSHPERDAELSHAESDLEEPAEGSGDTCKVGQSSSSGVIGADLGLGLALGPFCSPISRLERANSCSSSEESHSNIYSRKSFSISERMDKGRGSSRNPFQKARTGARLEGKTDSLSMRKMAKPSAFRSFDSRHNYT

Target ORF information:

RefSeq Version	XM_009301560.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301560.2

ORF Insert Sequence:

ATGTGGGGCC CTTTTCCGGA TGGCGGGAAC GTCCGGCCGG GAGCGATGAA CCAGCTGGAT 
GCTCCTCGGC CACTCAACTG GACCATACGG AAACTGTGCC ATGCAGCCTT CCTCCCGTCT 
GTGCGCCTGC TTAAGGCACA GAAATCATGG ATAGAAAGAG CGTTCAGCAA GAGAGAATGT 
GTGCACATTA TTGTAAGCAC CAAAGACCAC CATAGGTGCT GTTGTGGCCG TCTAATTGGT 
CAACATGTTG GCCTGCCCCC CAGCATCTCA TCCAATCAGA ATGAGAAGTC AGAACGCGTG 
CCAAAGAATG ACAGCTTGTC AGAGAAGTGG TCCATCAGCA AGCACACCCA GCTCAGTCCC 
ACCGACGCTT TTGGGACCAT CGAGTTCCAG GGAGGAGGAC ACTCTAATAA AGCAATGTAT 
GTACGAGTGT CTTACGACAC AAAACCAGAT TTACTGCTGC ATCTGATGAC AAAGGAGTGG 
CAACTGGAGC TTCCCAAGCT GCTCATCTCT GTTCACGGAG GACTTCAAAA CTTTGAGTTG 
CAGCCCAAAC TAAAGCAAGT CTTCGGCAAA GGTCTTATAA AGGCTGCAAT GACTACCGGA 
GCCTGGATCT TCACTGGAGG AGTTAATACT GGAGTCATCA GACATGTTGG TGATGCTCTG 
AAAGATCATG CTTCCAAATC AAGAGGGAAG ATCTGCACTA TCGGCATTGC CCCCTGGGGG 
ATTGTGGAGA ACCAGGAAGA CCTGGTGGGC AAAGATGTGG TCCGGCCATA CCAGACGATG 
TCTAACCCTC TTAGCAAGCT AACAGTACTC AACAGCCTAC ACTCACACTT CATTTTGGCT 
GATAATGGCA CCACCGGAAA GTACGGAGCA GAAGTCAAAC TGCGCAGGCA GCTGGAGAAG 
CACATCTCTC TGCAAAAGAT TAACACACGG ATTGGACAGG GTGTTCCAGT AGTGGCTCTG 
ATAGTGGAGG GGGGTCCGAA TGTCATCTCT ATCGTCCTGG AGTACCTAAG AGACACCCCT 
CCGGTGCCTG TTGTGGTGTG TGACGGCAGC GGTCGAGCCT CTGACATCCT GGCCTTTGGG 
CACAAGTACT CAGAGGAGGG CGGAATCATA AACGAATCTT TGAGAGACCA GTTATTAGTC 
ACCATTCAGA AGACGTTCAC ATACAGCCGG ACGCAGGCCC AGCATCTCTT CATCATTCTG 
ATGGAGTGCA TGAAGAAGAA AGAACTGATC ACAGTGTTTC GGATGGGATC AGAGGGACAC 
CAGGACATCG ATCTAGCCAT TCTAACAGCT CTTCTGAAAG GAGCGAATGC ATCAGCTCCA 
GATCAGCTAA GTCTGGCTTT GGCCTGGAAC AGAGTTGACA TCGCCCGGAG CCAGATCTTC 
ATTTACGGAC AGCAGTGGCC TGTTGGCTCT CTGGAGCAGT CTATGCTGGA CGCGCTGGTG 
CTGGACCGAG TGGACTTTGT AAAGCTGTTG ATTGAGAACG GAGTGAGCAT GCACCGTTTC 
CTGACACTCT CCAGACTTGA AGAGCTTTAT AATACGAGGC ACGGACCATC CAACACCTTG 
TACCACCTTG TCAGAGATGT GAAAAAGCAA GAATATCCAG GGTTCAGTTG GATCTACCTC 
AAGGGTAACC TGCCCCCCGA CTACCGCATT AGCCTGATTG ACATCGGATT GGTTATTGAA 
TACCTCATGG GTGGAGCCTA TCGCTGCAAT TACACCAGGA AGAGATTTCG GACCCTCTAT 
CACAACCTCT TTGGACCCAA AAGACCAAAA GCCCTGAAGC TGCTTGGAAT GGAGGATGAC 
ATGCCCATTA GACGAGGGCG TCAGAAGACT ACGCGGAAGC GAGAGGAGGA AGTAGATATT 
GACCTGGATG ACCCAGAAAT CAATCATTTC CCTTTCCCAT TCCATGAGCT GATGGTTTGG 
GCTGTGCTGA TGAAACGGCA GAAGATGGCA CTGTTCTTCT GGCAGCATGG AGAGGAGGCC 
ATGGCTAAAG CGCTGGTGGC CTGCAAGCTC TGTAAGGCAA TGGCTCACGA AGCTTCTGAG 
AACGACATGG TCGATGACAT TTCACAGGAG CTCAATCAAA ACTCAAGGGA GTTTGGTCAG 
CTGGCTGTTG AGCTGCTCGA TCAGTCCTAT AAGCAGGATG AACAGATGGC TATGAAGCTG 
TTGACCTACG AGCTGAAGAA CTGGAGTAAT GCCACCTGTC TGCAGCTGGC AGTGGCTGCC 
AAGCACCGAG ACTTCATCGC CCACACCTGT AGCCAGATGC TGCTCACAGA CATGTGGATG 
GGACGTCTGC GCATGCGCAA GAACTCTGGC CTGAAGGTTA TCTTGGGCCT GCTTCTCCCC 
CCTTCCATAT TGAGTCTGGA GTTTAAAAAT AAAGACGAGA TGTCATACAT GCCTCAGGAT 
CAGGACACAT ATCTGCAGGA GAAGGATGTG GATGAGCCCG AGAAACAAGC TAAGGAGAAA 
GAGGAGGAGG ACATGGAGTT CACAGTAAGA TCTTACTGTG AGACGCAGTA CAACTCCGTG 
GCTATGTTGG GAAACGTCAG TTCAGAAGCA TCGCGCAAGA AACAGGTGGA GGAGGTTCAG 
AACCGGCATC GGCTCATTCC ATTGGGTCGC AAAATATATG AGTTTTACAA CGCCCCTATC 
GTCAAGTTCT GGTTTCACAC GCTGGCGTAT GTAGGATACT TGATGCTCTT CAACTATATT 
GTGCTGGTGA AGATGGACCT GTGGCCTTCT CCTCAGGAGT GGATTGTTAT TGCCTACATA 
TTCACCAATG GCATCGAGAA GATGAGAGAG ATCCTGATGT CCGAACCTGG GAAGCTGCTT 
CAGAAGGTGA AAGTGTGGTT ACAGGAGTAC TGGAACATCA CTGACCTCAT GGCCATCCTC 
ATCTTCTCCA TCGGGATGGT GCTTCGTCTG CAGGACCCGC CGCTGATGAG CTACGGCAGG 
GTCATTTACT GCGTCAATAT CATTTATTGG TACATACGAC TCCTCGACAT CTTCGGAGTG 
AACAAATACC TGGGTCCATA CGTCATGATG ATCGGCAAGA TGATGATTGA CATGATGTAT 
TTTGTGATCA TCATGTTGGT GGTGCTGATG AGTTTCGGTG TGGCGCGTCA GGCCATCCTT 
AATCCCAATG AAGACCCATC CTGGATGCTG GCACGCAATA TCTTCTTCAT GCCATACTGG 
ATGATCTACG GAGAGGTGTT TGCTGACCAA ATCGACCCTC CTTGTGGTCA GAACATCACC 
ACAGAAGAAG GAGTTGTCAT GCCTCTTCCA CCCTGCAAAA CTGGAGCTTG GATTGTCCCT 
GCCATAATGG CCTGCTACCT GCTGGTGGCC AACATCCTGC TAGTTAACTT GCTCATTGCT 
GTCTTTAATA ACACTTTCTT TGAAGTCAAG TCCATTTCCA ACCAGGTGTG GAAGTTTCAG 
AGGTATCAGC TGATCATGAC CTTCCATGAA CGCCCGGTCC TTCCTCCTCC ACTCATCATC 
TTCAGCCACA TAACTATGGT TCTCAAACAC CTGTGCTGTC GCTGGAGGAA GCACGATGAG 
GATGAACGGG ACTATGGGCT GAAGCTTTTC ATCACTGAAG ATGAACTGAA GAAAGTGCAT 
GACTTTGAAG AGCAGTGCAT GGAGGAGTAT TTCAGAGAGA AAGATGACCG CTTCAATTCC 
TCCAATGACG AAAGGATACG TGTGACATCT GAAAGGGTGG AGAACATGGC GATGCGATTA 
GAGGAGGTGA ATGAGAGGGA ACATTTTATG AAAGCGTCCT TGCAGACTGT AGACATCCGG 
CTCGCCCAGA TGGAGGAGAT GATCGGCCGC ATTGCTGTGG CCCTAGAGAG AGTTGCTGGA 
ATGGACAGAG GCGAAGTTAA CAAAGCCCGG TCCAGGACAT CATCAGACTG CACTGATACC 
AATTACATCC TGCGCCAAAG CAGTTTCAAC AGCCAAGAAG GCAATTCCTA CAGGCTACAG 
GAGTCTCTGG AGCAGGGGGG CGACGAGTCC ATTTCTCCTA CTTCCCCAAC CGCCCTGGCT 
CCCCGTGTCC GCAGTCACTC CTTCTACGTG AGCCACTCTT CCAAAGACAG GAGTGGGGCG 
GACCGTGGAG AGGGCTTCTT TAAAGACAAG TTGTTCAGCC TCCACCGGGC CAACAGCTCA 
CAGTCTGTGT CCTCAGGAGC AGGTCCCAAA GAGTCCAAGC CAACCCCTTT GAATACTTTA 
TCCGTGCAGC AGCAGCTCCG CCCTTCTTCC TGCATAGACA TCTATGTTTC TGCCTCAGAG 
GACGTCCCGC CCACTGAGAG CTTCTTGGAT TCGGTTCGGA CAGTTCCAAC TCTGGCTCGA 
GACTCATCTC TTCATTCGGA GATCATGGAG GCGGTGCTCT CAGGGGGACG GGACTGTAGT 
GGAAGGGCAG GCGGCAGCGA AAGACAGTCG GATGGTACAG TCCTGTTTGA GGACAGCGCC 
GCCGCTGACC TATCGCTCTG CTCTGCCCAC CTGCTGCCGG ATTCCTTGCC CCCCTGGGAC 
CTGGACCCCT CACCGCCACC ATCAGCTGGT GTACTAGAGC GGTCCAAAAG TAGTCGCTTC 
CTGTCTACAG CAGGGCCTCT GTTTCTGGAC GAGCCTCATT TGGTTAAGTC ACACAGTCTG 
ATGTTCACAT CACGTGGGTA CTATGGTGGC ATGGGTGTGC AGGTGAAGGC GGCCGAATAC 
ACCAGCATCA CAGATTGCAT CGACACCCGT TGCGTCTCAA CCCCTTACCC TGTGCCAGAG 
CGGTCAGACT CACCTGGAGG ATCTTTCACC TTCGATAAGC CTCAAGACCT TGGCGTCTCG 
CACCCTGAGA GAGACGCTGA GCTCAGCCAC GCTGAGTCTG ACTTAGAGGA GCCAGCAGAG 
GGTTCGGGAG ACACATGTAA GGTGGGTCAG AGTAGCAGTA GTGGGGTAAT AGGTGCAGAC 
CTGGGCCTGG GCCTCGCACT AGGCCCGTTT TGCTCACCCA TCTCTAGACT AGAGCGAGCA 
AACAGCTGCT CCTCTTCAGA GGAGTCGCAC TCCAACATCT ACTCTCGAAA GAGCTTCTCA 
ATAAGCGAGA GAATGGACAA GGGCCGGGGG AGCTCCAGGA ACCCTTTCCA GAAGGCCAGA 
ACCGGGGCGA GGCTGGAGGG TAAGACAGAC TCACTGTCTA TGAGGAAAAT GGCCAAACCG 
TCAGCTTTCA GAAGTTTTGA CAGCAGACAC AACTACACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm3 ( XM_009301561.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299836.1 Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X15, mRNA.

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CloneID	ODa44721
Clone ID Related Accession (Same CDS sequence)	XM_009301561.3 , XM_009301561.2
Accession Version	XM_009301561.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily M member 3 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009301561.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGTAC GAGTGTCTTA CGACACAAAA CCAGATTTAC TGCTGCATCT GATGACAAAG 
GAGTGGCAAC TGGAGCTTCC CAAGCTGCTC ATCTCTGTTC ACGGAGGACT TCAAAACTTT 
GAGTTGCAGC CCAAACTAAA GCAAGTCTTC GGCAAAGGTC TTATAAAGGC TGCAATGACT 
ACCGGAGCCT GGATCTTCAC TGGAGGAGTT AATACTGGAG TCATCAGACA TGTTGGTGAT 
GCTCTGAAAG ATCATGCTTC CAAATCAAGA GGGAAGATCT GCACTATCGG CATTGCCCCC 
TGGGGGATTG TGGAGAACCA GGAAGACCTG GTGGGCAAAG ATGTGGTCCG GCCATACCAG 
ACGATGTCTA ACCCTCTTAG CAAGCTAACA GTACTCAACA GCCTACACTC ACACTTCATT 
TTGGCTGATA ATGGCACCAC CGGAAAGTAC GGAGCAGAAG TCAAACTGCG CAGGCAGCTG 
GAGAAGCACA TCTCTCTGCA AAAGATTAAC ACACGGATTG GACAGGGTGT TCCAGTAGTG 
GCTCTGATAG TGGAGGGGGG TCCGAATGTC ATCTCTATCG TCCTGGAGTA CCTAAGAGAC 
ACCCCTCCGG TGCCTGTTGT GGTGTGTGAC GGCAGCGGTC GAGCCTCTGA CATCCTGGCC 
TTTGGGCACA AGTACTCAGA GGAGGGCGGA ATCATAAACG AATCTTTGAG AGACCAGTTA 
TTAGTCACCA TTCAGAAGAC GTTCACATAC AGCCGGACGC AGGCCCAGCA TCTCTTCATC 
ATTCTGATGG AGTGCATGAA GAAGAAAGAA CTGATCACAG TGTTTCGGAT GGGATCAGAG 
GGACACCAGG ACATCGATCT AGCCATTCTA ACAGCTCTTC TGAAAGGAGC GAATGCATCA 
GCTCCAGATC AGCTAAGTCT GGCTTTGGCC TGGAACAGAG TTGACATCGC CCGGAGCCAG 
ATCTTCATTT ACGGACAGCA GTGGCCTGTT GGCTCTCTGG AGCAGTCTAT GCTGGACGCG 
CTGGTGCTGG ACCGAGTGGA CTTTGTAAAG CTGTTGATTG AGAACGGAGT GAGCATGCAC 
CGTTTCCTGA CACTCTCCAG ACTTGAAGAG CTTTATAATA CGAGGCACGG ACCATCCAAC 
ACCTTGTACC ACCTTGTCAG AGATGTGAAA AAGGGTAACC TGCCCCCCGA CTACCGCATT 
AGCCTGATTG ACATCGGATT GGTTATTGAA TACCTCATGG GTGGAGCCTA TCGCTGCAAT 
TACACCAGGA AGAGATTTCG GACCCTCTAT CACAACCTCT TTGGACCCAA AAGACCAAAA 
GCCCTGAAGC TGCTTGGAAT GGAGGATGAC ATGCCCATTA GACGAGGGCG TCAGAAGACT 
ACGCGGAAGC GAGAGGAGGA AGTAGATATT GACCTGGATG ACCCAGAAAT CAATCATTTC 
CCTTTCCCAT TCCATGAGCT GATGGTTTGG GCTGTGCTGA TGAAACGGCA GAAGATGGCA 
CTGTTCTTCT GGCAGCATGG AGAGGAGGCC ATGGCTAAAG CGCTGGTGGC CTGCAAGCTC 
TGTAAGGCAA TGGCTCACGA AGCTTCTGAG AACGACATGG TCGATGACAT TTCACAGGAG 
CTCAATCAAA ACTCAAGGGA GTTTGGTCAG CTGGCTGTTG AGCTGCTCGA TCAGTCCTAT 
AAGCAGGATG AACAGATGGC TATGAAGCTG TTGACCTACG AGCTGAAGAA CTGGAGTAAT 
GCCACCTGTC TGCAGCTGGC AGTGGCTGCC AAGCACCGAG ACTTCATCGC CCACACCTGT 
AGCCAGATGC TGCTCACAGA CATGTGGATG GGACGTCTGC GCATGCGCAA GAACTCTGGC 
CTGAAGGTTA TCTTGGGCCT GCTTCTCCCC CCTTCCATAT TGAGTCTGGA GTTTAAAAAT 
AAAGACGAGA TGTCATACAT GCCTCAGGAT CAGGACACAT ATCTGCAGGA GAAGGATGTG 
GATGAGCCCG AGAAACAAGC TAAGGAGAAA GAGGAGGAGG ACATGGAGTT CACAGTAAGA 
TCTTACTGTG AGACGCAGTA CAACTCCGTG GCTATGTTGG GAAACGTCAG TTCAGAAGCA 
TCGCGCAAGA AACAGGTGGA GGAGGTTCAG AACCGGCATC GGCTCATTCC ATTGGGTCGC 
AAAATATATG AGTTTTACAA CGCCCCTATC GTCAAGTTCT GGTTTCACAC GCTGGCGTAT 
GTAGGATACT TGATGCTCTT CAACTATATT GTGCTGGTGA AGATGGACCT GTGGCCTTCT 
CCTCAGGAGT GGATTGTTAT TGCCTACATA TTCACCAATG GCATCGAGAA GATGAGAGAG 
ATCCTGATGT CCGAACCTGG GAAGCTGCTT CAGAAGGTGA AAGTGTGGTT ACAGGAGTAC 
TGGAACATCA CTGACCTCAT GGCCATCCTC ATCTTCTCCA TCGGGATGGT GCTTCGTCTG 
CAGGACCCGC CGCTGATGAG CTACGGCAGG GTCATTTACT GCGTCAATAT CATTTATTGG 
TACATACGAC TCCTCGACAT CTTCGGAGTG AACAAATACC TGGGTCCATA CGTCATGATG 
ATCGGCAAGA TGATGATTGA CATGATGTAT TTTGTGATCA TCATGTTGGT GGTGCTGATG 
AGTTTCGGTG TGGCGCGTCA GGCCATCCTT AATCCCAATG AAGACCCATC CTGGATGCTG 
GCACGCAATA TCTTCTTCAT GCCATACTGG ATGATCTACG GAGAGGTGTT TGCTGACCAA 
ATCGACCCTC CTTGTGGTCA GAACATCACC ACAGAAGAAG GAGTTGTCAT GCCTCTTCCA 
CCCTGCAAAA CTGGAGCTTG GATTGTCCCT GCCATAATGG CCTGCTACCT GCTGGTGGCC 
AACATCCTGC TAGTTAACTT GCTCATTGCT GTCTTTAATA ACACTTTCTT TGAAGTCAAG 
TCCATTTCCA ACCAGGTGTG GAAGTTTCAG AGGTATCAGC TGATCATGAC CTTCCATGAA 
CGCCCGGTCC TTCCTCCTCC ACTCATCATC TTCAGCCACA TAACTATGGT TCTCAAACAC 
CTGTGCTGTC GCTGGAGGAA GCACGATGAG GATGAACGGG ACTATGGGCT GAAGCTTTTC 
ATCACTGAAG ATGAACTGAA GAAAGTGCAT GACTTTGAAG AGCAGTGCAT GGAGGAGTAT 
TTCAGAGAGA AAGATGACCG CTTCAATTCC TCCAATGACG AAAGGATACG TGTGACATCT 
GAAAGGGTGG AGAACATGGC GATGCGATTA GAGGAGGTGA ATGAGAGGGA ACATTTTATG 
AAAGCGTCCT TGCAGACTGT AGACATCCGG CTCGCCCAGA TGGAGGAGAT GATCGGCCGC 
ATTGCTGTGG CCCTAGAGAG AGTTGCTGGA ATGGACAGAG GCGAAGTTAA CAAAGCCCGG 
TCCAGGACAT CATCAGACTG CACTGATACC AATTACATCC TGCGCCAAAG CAGTTTCAAC 
AGCCAAGAAG GCAATTCCTA CAGGCTACAG GAGTCTCTGG AGCAGGGGGG CGACGAGTCC 
ATTTCTCCTA CTTCCCCAAC CGCCCTGGCT CCCCGTGTCC GCAGTCACTC CTTCTACGTG 
AGCCACTCTT CCAAAGACAG GAGTGGGGCG GACCGTGGAG AGGGCTTCTT TAAAGACAAG 
TTGTTCAGCC TCCACCGGGC CAACAGCTCA CAGTCTGTGT CCTCAGGAGC AGGTCCCAAA 
GAGTCCAAGC CAACCCCTTT GAATACTTTA TCCGTGCAGC AGCAGCTCCG CCCTTCTTCC 
TGCATAGACA TCTATGTTTC TGCCTCAGAG GACGTCCCGC CCACTGAGAG CTTCTTGGAT 
TCGGTTCGGA CAGTTCCAAC TCTGGCTCGA GACTCATCTC TTCATTCGGA GATCATGGAG 
GCGGTGCTCT CAGGGGGACG GGACTGTAGT GGAAGGGCAG GCGGCAGCGA AAGACAGTCG 
GATGGTACAG TCCTGTTTGA GGACAGCGCC GCCGCTGACC TATCGCTCTG CTCTGCCCAC 
CTGCTGCCGG ATTCCTTGCC CCCCTGGGAC CTGGACCCCT CACCGCCACC ATCAGCTGGT 
GTACTAGAGC GGTCCAAAAG TAGTCGCTTC CTGTCTACAG CAGGGCCTCT GTTTCTGGAC 
GAGCCTCATT TGGTTAAGTC ACACAGTCTG ATGTTCACAT CACGTGGGTA CTATGGTGGC 
ATGGGTGTGC AGGTGAAGGC GGCCGAATAC ACCAGCATCA CAGATTGCAT CGACACCCGT 
TGCGTCTCAA CCCCTTACCC TGTGCCAGAG CGGTCAGACT CACCTGGAGG ATCTTTCACC 
TTCGATAAGC CTCAAGACCT TGGCGTCTCG CACCCTGAGA GAGACGCTGA GCTCAGCCAC 
GCTGAGTCTG ACTTAGAGGA GCCAGCAGAG GGTTCGGGAG ACACATGTAA GGTGGGTCAG 
AGTAGCAGTA GTGGGGTAAT AGGTGCAGAC CTGGGCCTGG GCCTCGCACT AGGCCCGTTT 
TGCTCACCCA TCTCTAGACT AGAGCGAGCA AACAGCTGCT CCTCTTCAGA GGAGTCGCAC 
TCCAACATCT ACTCTCGAAA GAGCTTCTCA ATAAGCGAGA GAATGGACAA GGGCCGGGGG 
AGCTCCAGGA ACCCTTTCCA GAAGGCCAGA ACCGGGGCGA GGCTGGAGGG TAAGACAGAC 
TCACTGTCTA TGAGGAAAAT GGCCAAACCG TCAGCTTTCA GAAGTTTTGA CAGCAGACAC 
AACTACACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299836.1
CDS	434..5185
Translation	MYVRVSYDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLVGKDVVRPYQTMSNPLSKLTVLNSLHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGIINESLRDQLLVTIQKTFTYSRTQAQHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQSMLDALVLDRVDFVKLLIENGVSMHRFLTLSRLEELYNTRHGPSNTLYHLVRDVKKGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDMPIRRGRQKTTRKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNQNSREFGQLAVELLDQSYKQDEQMAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGLLLPPSILSLEFKNKDEMSYMPQDQDTYLQEKDVDEPEKQAKEKEEEDMEFTVRSYCETQYNSVAMLGNVSSEASRKKQVEEVQNRHRLIPLGRKIYEFYNAPIVKFWFHTLAYVGYLMLFNYIVLVKMDLWPSPQEWIVIAYIFTNGIEKMREILMSEPGKLLQKVKVWLQEYWNITDLMAILIFSIGMVLRLQDPPLMSYGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDPPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHITMVLKHLCCRWRKHDEDERDYGLKLFITEDELKKVHDFEEQCMEEYFREKDDRFNSSNDERIRVTSERVENMAMRLEEVNEREHFMKASLQTVDIRLAQMEEMIGRIAVALERVAGMDRGEVNKARSRTSSDCTDTNYILRQSSFNSQEGNSYRLQESLEQGGDESISPTSPTALAPRVRSHSFYVSHSSKDRSGADRGEGFFKDKLFSLHRANSSQSVSSGAGPKESKPTPLNTLSVQQQLRPSSCIDIYVSASEDVPPTESFLDSVRTVPTLARDSSLHSEIMEAVLSGGRDCSGRAGGSERQSDGTVLFEDSAAADLSLCSAHLLPDSLPPWDLDPSPPPSAGVLERSKSSRFLSTAGPLFLDEPHLVKSHSLMFTSRGYYGGMGVQVKAAEYTSITDCIDTRCVSTPYPVPERSDSPGGSFTFDKPQDLGVSHPERDAELSHAESDLEEPAEGSGDTCKVGQSSSSGVIGADLGLGLALGPFCSPISRLERANSCSSSEESHSNIYSRKSFSISERMDKGRGSSRNPFQKARTGARLEGKTDSLSMRKMAKPSAFRSFDSRHNYT

Target ORF information:

RefSeq Version	XM_009301561.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301561.3

ORF Insert Sequence:

ATGTATGTAC GAGTGTCTTA CGACACAAAA CCAGATTTAC TGCTGCATCT GATGACAAAG 
GAGTGGCAAC TGGAGCTTCC CAAGCTGCTC ATCTCTGTTC ACGGAGGACT TCAAAACTTT 
GAGTTGCAGC CCAAACTAAA GCAAGTCTTC GGCAAAGGTC TTATAAAGGC TGCAATGACT 
ACCGGAGCCT GGATCTTCAC TGGAGGAGTT AATACTGGAG TCATCAGACA TGTTGGTGAT 
GCTCTGAAAG ATCATGCTTC CAAATCAAGA GGGAAGATCT GCACTATCGG CATTGCCCCC 
TGGGGGATTG TGGAGAACCA GGAAGACCTG GTGGGCAAAG ATGTGGTCCG GCCATACCAG 
ACGATGTCTA ACCCTCTTAG CAAGCTAACA GTACTCAACA GCCTACACTC ACACTTCATT 
TTGGCTGATA ATGGCACCAC CGGAAAGTAC GGAGCAGAAG TCAAACTGCG CAGGCAGCTG 
GAGAAGCACA TCTCTCTGCA AAAGATTAAC ACACGGATTG GACAGGGTGT TCCAGTAGTG 
GCTCTGATAG TGGAGGGGGG TCCGAATGTC ATCTCTATCG TCCTGGAGTA CCTAAGAGAC 
ACCCCTCCGG TGCCTGTTGT GGTGTGTGAC GGCAGCGGTC GAGCCTCTGA CATCCTGGCC 
TTTGGGCACA AGTACTCAGA GGAGGGCGGA ATCATAAACG AATCTTTGAG AGACCAGTTA 
TTAGTCACCA TTCAGAAGAC GTTCACATAC AGCCGGACGC AGGCCCAGCA TCTCTTCATC 
ATTCTGATGG AGTGCATGAA GAAGAAAGAA CTGATCACAG TGTTTCGGAT GGGATCAGAG 
GGACACCAGG ACATCGATCT AGCCATTCTA ACAGCTCTTC TGAAAGGAGC GAATGCATCA 
GCTCCAGATC AGCTAAGTCT GGCTTTGGCC TGGAACAGAG TTGACATCGC CCGGAGCCAG 
ATCTTCATTT ACGGACAGCA GTGGCCTGTT GGCTCTCTGG AGCAGTCTAT GCTGGACGCG 
CTGGTGCTGG ACCGAGTGGA CTTTGTAAAG CTGTTGATTG AGAACGGAGT GAGCATGCAC 
CGTTTCCTGA CACTCTCCAG ACTTGAAGAG CTTTATAATA CGAGGCACGG ACCATCCAAC 
ACCTTGTACC ACCTTGTCAG AGATGTGAAA AAGGGTAACC TGCCCCCCGA CTACCGCATT 
AGCCTGATTG ACATCGGATT GGTTATTGAA TACCTCATGG GTGGAGCCTA TCGCTGCAAT 
TACACCAGGA AGAGATTTCG GACCCTCTAT CACAACCTCT TTGGACCCAA AAGACCAAAA 
GCCCTGAAGC TGCTTGGAAT GGAGGATGAC ATGCCCATTA GACGAGGGCG TCAGAAGACT 
ACGCGGAAGC GAGAGGAGGA AGTAGATATT GACCTGGATG ACCCAGAAAT CAATCATTTC 
CCTTTCCCAT TCCATGAGCT GATGGTTTGG GCTGTGCTGA TGAAACGGCA GAAGATGGCA 
CTGTTCTTCT GGCAGCATGG AGAGGAGGCC ATGGCTAAAG CGCTGGTGGC CTGCAAGCTC 
TGTAAGGCAA TGGCTCACGA AGCTTCTGAG AACGACATGG TCGATGACAT TTCACAGGAG 
CTCAATCAAA ACTCAAGGGA GTTTGGTCAG CTGGCTGTTG AGCTGCTCGA TCAGTCCTAT 
AAGCAGGATG AACAGATGGC TATGAAGCTG TTGACCTACG AGCTGAAGAA CTGGAGTAAT 
GCCACCTGTC TGCAGCTGGC AGTGGCTGCC AAGCACCGAG ACTTCATCGC CCACACCTGT 
AGCCAGATGC TGCTCACAGA CATGTGGATG GGACGTCTGC GCATGCGCAA GAACTCTGGC 
CTGAAGGTTA TCTTGGGCCT GCTTCTCCCC CCTTCCATAT TGAGTCTGGA GTTTAAAAAT 
AAAGACGAGA TGTCATACAT GCCTCAGGAT CAGGACACAT ATCTGCAGGA GAAGGATGTG 
GATGAGCCCG AGAAACAAGC TAAGGAGAAA GAGGAGGAGG ACATGGAGTT CACAGTAAGA 
TCTTACTGTG AGACGCAGTA CAACTCCGTG GCTATGTTGG GAAACGTCAG TTCAGAAGCA 
TCGCGCAAGA AACAGGTGGA GGAGGTTCAG AACCGGCATC GGCTCATTCC ATTGGGTCGC 
AAAATATATG AGTTTTACAA CGCCCCTATC GTCAAGTTCT GGTTTCACAC GCTGGCGTAT 
GTAGGATACT TGATGCTCTT CAACTATATT GTGCTGGTGA AGATGGACCT GTGGCCTTCT 
CCTCAGGAGT GGATTGTTAT TGCCTACATA TTCACCAATG GCATCGAGAA GATGAGAGAG 
ATCCTGATGT CCGAACCTGG GAAGCTGCTT CAGAAGGTGA AAGTGTGGTT ACAGGAGTAC 
TGGAACATCA CTGACCTCAT GGCCATCCTC ATCTTCTCCA TCGGGATGGT GCTTCGTCTG 
CAGGACCCGC CGCTGATGAG CTACGGCAGG GTCATTTACT GCGTCAATAT CATTTATTGG 
TACATACGAC TCCTCGACAT CTTCGGAGTG AACAAATACC TGGGTCCATA CGTCATGATG 
ATCGGCAAGA TGATGATTGA CATGATGTAT TTTGTGATCA TCATGTTGGT GGTGCTGATG 
AGTTTCGGTG TGGCGCGTCA GGCCATCCTT AATCCCAATG AAGACCCATC CTGGATGCTG 
GCACGCAATA TCTTCTTCAT GCCATACTGG ATGATCTACG GAGAGGTGTT TGCTGACCAA 
ATCGACCCTC CTTGTGGTCA GAACATCACC ACAGAAGAAG GAGTTGTCAT GCCTCTTCCA 
CCCTGCAAAA CTGGAGCTTG GATTGTCCCT GCCATAATGG CCTGCTACCT GCTGGTGGCC 
AACATCCTGC TAGTTAACTT GCTCATTGCT GTCTTTAATA ACACTTTCTT TGAAGTCAAG 
TCCATTTCCA ACCAGGTGTG GAAGTTTCAG AGGTATCAGC TGATCATGAC CTTCCATGAA 
CGCCCGGTCC TTCCTCCTCC ACTCATCATC TTCAGCCACA TAACTATGGT TCTCAAACAC 
CTGTGCTGTC GCTGGAGGAA GCACGATGAG GATGAACGGG ACTATGGGCT GAAGCTTTTC 
ATCACTGAAG ATGAACTGAA GAAAGTGCAT GACTTTGAAG AGCAGTGCAT GGAGGAGTAT 
TTCAGAGAGA AAGATGACCG CTTCAATTCC TCCAATGACG AAAGGATACG TGTGACATCT 
GAAAGGGTGG AGAACATGGC GATGCGATTA GAGGAGGTGA ATGAGAGGGA ACATTTTATG 
AAAGCGTCCT TGCAGACTGT AGACATCCGG CTCGCCCAGA TGGAGGAGAT GATCGGCCGC 
ATTGCTGTGG CCCTAGAGAG AGTTGCTGGA ATGGACAGAG GCGAAGTTAA CAAAGCCCGG 
TCCAGGACAT CATCAGACTG CACTGATACC AATTACATCC TGCGCCAAAG CAGTTTCAAC 
AGCCAAGAAG GCAATTCCTA CAGGCTACAG GAGTCTCTGG AGCAGGGGGG CGACGAGTCC 
ATTTCTCCTA CTTCCCCAAC CGCCCTGGCT CCCCGTGTCC GCAGTCACTC CTTCTACGTG 
AGCCACTCTT CCAAAGACAG GAGTGGGGCG GACCGTGGAG AGGGCTTCTT TAAAGACAAG 
TTGTTCAGCC TCCACCGGGC CAACAGCTCA CAGTCTGTGT CCTCAGGAGC AGGTCCCAAA 
GAGTCCAAGC CAACCCCTTT GAATACTTTA TCCGTGCAGC AGCAGCTCCG CCCTTCTTCC 
TGCATAGACA TCTATGTTTC TGCCTCAGAG GACGTCCCGC CCACTGAGAG CTTCTTGGAT 
TCGGTTCGGA CAGTTCCAAC TCTGGCTCGA GACTCATCTC TTCATTCGGA GATCATGGAG 
GCGGTGCTCT CAGGGGGACG GGACTGTAGT GGAAGGGCAG GCGGCAGCGA AAGACAGTCG 
GATGGTACAG TCCTGTTTGA GGACAGCGCC GCCGCTGACC TATCGCTCTG CTCTGCCCAC 
CTGCTGCCGG ATTCCTTGCC CCCCTGGGAC CTGGACCCCT CACCGCCACC ATCAGCTGGT 
GTACTAGAGC GGTCCAAAAG TAGTCGCTTC CTGTCTACAG CAGGGCCTCT GTTTCTGGAC 
GAGCCTCATT TGGTTAAGTC ACACAGTCTG ATGTTCACAT CACGTGGGTA CTATGGTGGC 
ATGGGTGTGC AGGTGAAGGC GGCCGAATAC ACCAGCATCA CAGATTGCAT CGACACCCGT 
TGCGTCTCAA CCCCTTACCC TGTGCCAGAG CGGTCAGACT CACCTGGAGG ATCTTTCACC 
TTCGATAAGC CTCAAGACCT TGGCGTCTCG CACCCTGAGA GAGACGCTGA GCTCAGCCAC 
GCTGAGTCTG ACTTAGAGGA GCCAGCAGAG GGTTCGGGAG ACACATGTAA GGTGGGTCAG 
AGTAGCAGTA GTGGGGTAAT AGGTGCAGAC CTGGGCCTGG GCCTCGCACT AGGCCCGTTT 
TGCTCACCCA TCTCTAGACT AGAGCGAGCA AACAGCTGCT CCTCTTCAGA GGAGTCGCAC 
TCCAACATCT ACTCTCGAAA GAGCTTCTCA ATAAGCGAGA GAATGGACAA GGGCCGGGGG 
AGCTCCAGGA ACCCTTTCCA GAAGGCCAGA ACCGGGGCGA GGCTGGAGGG TAAGACAGAC 
TCACTGTCTA TGAGGAAAAT GGCCAAACCG TCAGCTTTCA GAAGTTTTGA CAGCAGACAC 
AACTACACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm3 ( XM_009301561.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299836.1 Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X9, mRNA.

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CloneID	ODa44721
Clone ID Related Accession (Same CDS sequence)	XM_009301561.3 , XM_009301561.2
Accession Version	XM_009301561.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transient receptor potential cation channel subfamily M member 3 isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301561.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATGTAC GAGTGTCTTA CGACACAAAA CCAGATTTAC TGCTGCATCT GATGACAAAG 
GAGTGGCAAC TGGAGCTTCC CAAGCTGCTC ATCTCTGTTC ACGGAGGACT TCAAAACTTT 
GAGTTGCAGC CCAAACTAAA GCAAGTCTTC GGCAAAGGTC TTATAAAGGC TGCAATGACT 
ACCGGAGCCT GGATCTTCAC TGGAGGAGTT AATACTGGAG TCATCAGACA TGTTGGTGAT 
GCTCTGAAAG ATCATGCTTC CAAATCAAGA GGGAAGATCT GCACTATCGG CATTGCCCCC 
TGGGGGATTG TGGAGAACCA GGAAGACCTG GTGGGCAAAG ATGTGGTCCG GCCATACCAG 
ACGATGTCTA ACCCTCTTAG CAAGCTAACA GTACTCAACA GCCTACACTC ACACTTCATT 
TTGGCTGATA ATGGCACCAC CGGAAAGTAC GGAGCAGAAG TCAAACTGCG CAGGCAGCTG 
GAGAAGCACA TCTCTCTGCA AAAGATTAAC ACACGGATTG GACAGGGTGT TCCAGTAGTG 
GCTCTGATAG TGGAGGGGGG TCCGAATGTC ATCTCTATCG TCCTGGAGTA CCTAAGAGAC 
ACCCCTCCGG TGCCTGTTGT GGTGTGTGAC GGCAGCGGTC GAGCCTCTGA CATCCTGGCC 
TTTGGGCACA AGTACTCAGA GGAGGGCGGA ATCATAAACG AATCTTTGAG AGACCAGTTA 
TTAGTCACCA TTCAGAAGAC GTTCACATAC AGCCGGACGC AGGCCCAGCA TCTCTTCATC 
ATTCTGATGG AGTGCATGAA GAAGAAAGAA CTGATCACAG TGTTTCGGAT GGGATCAGAG 
GGACACCAGG ACATCGATCT AGCCATTCTA ACAGCTCTTC TGAAAGGAGC GAATGCATCA 
GCTCCAGATC AGCTAAGTCT GGCTTTGGCC TGGAACAGAG TTGACATCGC CCGGAGCCAG 
ATCTTCATTT ACGGACAGCA GTGGCCTGTT GGCTCTCTGG AGCAGTCTAT GCTGGACGCG 
CTGGTGCTGG ACCGAGTGGA CTTTGTAAAG CTGTTGATTG AGAACGGAGT GAGCATGCAC 
CGTTTCCTGA CACTCTCCAG ACTTGAAGAG CTTTATAATA CGAGGCACGG ACCATCCAAC 
ACCTTGTACC ACCTTGTCAG AGATGTGAAA AAGGGTAACC TGCCCCCCGA CTACCGCATT 
AGCCTGATTG ACATCGGATT GGTTATTGAA TACCTCATGG GTGGAGCCTA TCGCTGCAAT 
TACACCAGGA AGAGATTTCG GACCCTCTAT CACAACCTCT TTGGACCCAA AAGACCAAAA 
GCCCTGAAGC TGCTTGGAAT GGAGGATGAC ATGCCCATTA GACGAGGGCG TCAGAAGACT 
ACGCGGAAGC GAGAGGAGGA AGTAGATATT GACCTGGATG ACCCAGAAAT CAATCATTTC 
CCTTTCCCAT TCCATGAGCT GATGGTTTGG GCTGTGCTGA TGAAACGGCA GAAGATGGCA 
CTGTTCTTCT GGCAGCATGG AGAGGAGGCC ATGGCTAAAG CGCTGGTGGC CTGCAAGCTC 
TGTAAGGCAA TGGCTCACGA AGCTTCTGAG AACGACATGG TCGATGACAT TTCACAGGAG 
CTCAATCAAA ACTCAAGGGA GTTTGGTCAG CTGGCTGTTG AGCTGCTCGA TCAGTCCTAT 
AAGCAGGATG AACAGATGGC TATGAAGCTG TTGACCTACG AGCTGAAGAA CTGGAGTAAT 
GCCACCTGTC TGCAGCTGGC AGTGGCTGCC AAGCACCGAG ACTTCATCGC CCACACCTGT 
AGCCAGATGC TGCTCACAGA CATGTGGATG GGACGTCTGC GCATGCGCAA GAACTCTGGC 
CTGAAGGTTA TCTTGGGCCT GCTTCTCCCC CCTTCCATAT TGAGTCTGGA GTTTAAAAAT 
AAAGACGAGA TGTCATACAT GCCTCAGGAT CAGGACACAT ATCTGCAGGA GAAGGATGTG 
GATGAGCCCG AGAAACAAGC TAAGGAGAAA GAGGAGGAGG ACATGGAGTT CACAGTAAGA 
TCTTACTGTG AGACGCAGTA CAACTCCGTG GCTATGTTGG GAAACGTCAG TTCAGAAGCA 
TCGCGCAAGA AACAGGTGGA GGAGGTTCAG AACCGGCATC GGCTCATTCC ATTGGGTCGC 
AAAATATATG AGTTTTACAA CGCCCCTATC GTCAAGTTCT GGTTTCACAC GCTGGCGTAT 
GTAGGATACT TGATGCTCTT CAACTATATT GTGCTGGTGA AGATGGACCT GTGGCCTTCT 
CCTCAGGAGT GGATTGTTAT TGCCTACATA TTCACCAATG GCATCGAGAA GATGAGAGAG 
ATCCTGATGT CCGAACCTGG GAAGCTGCTT CAGAAGGTGA AAGTGTGGTT ACAGGAGTAC 
TGGAACATCA CTGACCTCAT GGCCATCCTC ATCTTCTCCA TCGGGATGGT GCTTCGTCTG 
CAGGACCCGC CGCTGATGAG CTACGGCAGG GTCATTTACT GCGTCAATAT CATTTATTGG 
TACATACGAC TCCTCGACAT CTTCGGAGTG AACAAATACC TGGGTCCATA CGTCATGATG 
ATCGGCAAGA TGATGATTGA CATGATGTAT TTTGTGATCA TCATGTTGGT GGTGCTGATG 
AGTTTCGGTG TGGCGCGTCA GGCCATCCTT AATCCCAATG AAGACCCATC CTGGATGCTG 
GCACGCAATA TCTTCTTCAT GCCATACTGG ATGATCTACG GAGAGGTGTT TGCTGACCAA 
ATCGACCCTC CTTGTGGTCA GAACATCACC ACAGAAGAAG GAGTTGTCAT GCCTCTTCCA 
CCCTGCAAAA CTGGAGCTTG GATTGTCCCT GCCATAATGG CCTGCTACCT GCTGGTGGCC 
AACATCCTGC TAGTTAACTT GCTCATTGCT GTCTTTAATA ACACTTTCTT TGAAGTCAAG 
TCCATTTCCA ACCAGGTGTG GAAGTTTCAG AGGTATCAGC TGATCATGAC CTTCCATGAA 
CGCCCGGTCC TTCCTCCTCC ACTCATCATC TTCAGCCACA TAACTATGGT TCTCAAACAC 
CTGTGCTGTC GCTGGAGGAA GCACGATGAG GATGAACGGG ACTATGGGCT GAAGCTTTTC 
ATCACTGAAG ATGAACTGAA GAAAGTGCAT GACTTTGAAG AGCAGTGCAT GGAGGAGTAT 
TTCAGAGAGA AAGATGACCG CTTCAATTCC TCCAATGACG AAAGGATACG TGTGACATCT 
GAAAGGGTGG AGAACATGGC GATGCGATTA GAGGAGGTGA ATGAGAGGGA ACATTTTATG 
AAAGCGTCCT TGCAGACTGT AGACATCCGG CTCGCCCAGA TGGAGGAGAT GATCGGCCGC 
ATTGCTGTGG CCCTAGAGAG AGTTGCTGGA ATGGACAGAG GCGAAGTTAA CAAAGCCCGG 
TCCAGGACAT CATCAGACTG CACTGATACC AATTACATCC TGCGCCAAAG CAGTTTCAAC 
AGCCAAGAAG GCAATTCCTA CAGGCTACAG GAGTCTCTGG AGCAGGGGGG CGACGAGTCC 
ATTTCTCCTA CTTCCCCAAC CGCCCTGGCT CCCCGTGTCC GCAGTCACTC CTTCTACGTG 
AGCCACTCTT CCAAAGACAG GAGTGGGGCG GACCGTGGAG AGGGCTTCTT TAAAGACAAG 
TTGTTCAGCC TCCACCGGGC CAACAGCTCA CAGTCTGTGT CCTCAGGAGC AGGTCCCAAA 
GAGTCCAAGC CAACCCCTTT GAATACTTTA TCCGTGCAGC AGCAGCTCCG CCCTTCTTCC 
TGCATAGACA TCTATGTTTC TGCCTCAGAG GACGTCCCGC CCACTGAGAG CTTCTTGGAT 
TCGGTTCGGA CAGTTCCAAC TCTGGCTCGA GACTCATCTC TTCATTCGGA GATCATGGAG 
GCGGTGCTCT CAGGGGGACG GGACTGTAGT GGAAGGGCAG GCGGCAGCGA AAGACAGTCG 
GATGGTACAG TCCTGTTTGA GGACAGCGCC GCCGCTGACC TATCGCTCTG CTCTGCCCAC 
CTGCTGCCGG ATTCCTTGCC CCCCTGGGAC CTGGACCCCT CACCGCCACC ATCAGCTGGT 
GTACTAGAGC GGTCCAAAAG TAGTCGCTTC CTGTCTACAG CAGGGCCTCT GTTTCTGGAC 
GAGCCTCATT TGGTTAAGTC ACACAGTCTG ATGTTCACAT CACGTGGGTA CTATGGTGGC 
ATGGGTGTGC AGGTGAAGGC GGCCGAATAC ACCAGCATCA CAGATTGCAT CGACACCCGT 
TGCGTCTCAA CCCCTTACCC TGTGCCAGAG CGGTCAGACT CACCTGGAGG ATCTTTCACC 
TTCGATAAGC CTCAAGACCT TGGCGTCTCG CACCCTGAGA GAGACGCTGA GCTCAGCCAC 
GCTGAGTCTG ACTTAGAGGA GCCAGCAGAG GGTTCGGGAG ACACATGTAA GGTGGGTCAG 
AGTAGCAGTA GTGGGGTAAT AGGTGCAGAC CTGGGCCTGG GCCTCGCACT AGGCCCGTTT 
TGCTCACCCA TCTCTAGACT AGAGCGAGCA AACAGCTGCT CCTCTTCAGA GGAGTCGCAC 
TCCAACATCT ACTCTCGAAA GAGCTTCTCA ATAAGCGAGA GAATGGACAA GGGCCGGGGG 
AGCTCCAGGA ACCCTTTCCA GAAGGCCAGA ACCGGGGCGA GGCTGGAGGG TAAGACAGAC 
TCACTGTCTA TGAGGAAAAT GGCCAAACCG TCAGCTTTCA GAAGTTTTGA CAGCAGACAC 
AACTACACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299836.1
CDS	465..5216
Translation	MYVRVSYDTKPDLLLHLMTKEWQLELPKLLISVHGGLQNFELQPKLKQVFGKGLIKAAMTTGAWIFTGGVNTGVIRHVGDALKDHASKSRGKICTIGIAPWGIVENQEDLVGKDVVRPYQTMSNPLSKLTVLNSLHSHFILADNGTTGKYGAEVKLRRQLEKHISLQKINTRIGQGVPVVALIVEGGPNVISIVLEYLRDTPPVPVVVCDGSGRASDILAFGHKYSEEGGIINESLRDQLLVTIQKTFTYSRTQAQHLFIILMECMKKKELITVFRMGSEGHQDIDLAILTALLKGANASAPDQLSLALAWNRVDIARSQIFIYGQQWPVGSLEQSMLDALVLDRVDFVKLLIENGVSMHRFLTLSRLEELYNTRHGPSNTLYHLVRDVKKGNLPPDYRISLIDIGLVIEYLMGGAYRCNYTRKRFRTLYHNLFGPKRPKALKLLGMEDDMPIRRGRQKTTRKREEEVDIDLDDPEINHFPFPFHELMVWAVLMKRQKMALFFWQHGEEAMAKALVACKLCKAMAHEASENDMVDDISQELNQNSREFGQLAVELLDQSYKQDEQMAMKLLTYELKNWSNATCLQLAVAAKHRDFIAHTCSQMLLTDMWMGRLRMRKNSGLKVILGLLLPPSILSLEFKNKDEMSYMPQDQDTYLQEKDVDEPEKQAKEKEEEDMEFTVRSYCETQYNSVAMLGNVSSEASRKKQVEEVQNRHRLIPLGRKIYEFYNAPIVKFWFHTLAYVGYLMLFNYIVLVKMDLWPSPQEWIVIAYIFTNGIEKMREILMSEPGKLLQKVKVWLQEYWNITDLMAILIFSIGMVLRLQDPPLMSYGRVIYCVNIIYWYIRLLDIFGVNKYLGPYVMMIGKMMIDMMYFVIIMLVVLMSFGVARQAILNPNEDPSWMLARNIFFMPYWMIYGEVFADQIDPPCGQNITTEEGVVMPLPPCKTGAWIVPAIMACYLLVANILLVNLLIAVFNNTFFEVKSISNQVWKFQRYQLIMTFHERPVLPPPLIIFSHITMVLKHLCCRWRKHDEDERDYGLKLFITEDELKKVHDFEEQCMEEYFREKDDRFNSSNDERIRVTSERVENMAMRLEEVNEREHFMKASLQTVDIRLAQMEEMIGRIAVALERVAGMDRGEVNKARSRTSSDCTDTNYILRQSSFNSQEGNSYRLQESLEQGGDESISPTSPTALAPRVRSHSFYVSHSSKDRSGADRGEGFFKDKLFSLHRANSSQSVSSGAGPKESKPTPLNTLSVQQQLRPSSCIDIYVSASEDVPPTESFLDSVRTVPTLARDSSLHSEIMEAVLSGGRDCSGRAGGSERQSDGTVLFEDSAAADLSLCSAHLLPDSLPPWDLDPSPPPSAGVLERSKSSRFLSTAGPLFLDEPHLVKSHSLMFTSRGYYGGMGVQVKAAEYTSITDCIDTRCVSTPYPVPERSDSPGGSFTFDKPQDLGVSHPERDAELSHAESDLEEPAEGSGDTCKVGQSSSSGVIGADLGLGLALGPFCSPISRLERANSCSSSEESHSNIYSRKSFSISERMDKGRGSSRNPFQKARTGARLEGKTDSLSMRKMAKPSAFRSFDSRHNYT

Target ORF information:

RefSeq Version	XM_009301561.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 3 (trpm3), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301561.2

ORF Insert Sequence:

ATGTATGTAC GAGTGTCTTA CGACACAAAA CCAGATTTAC TGCTGCATCT GATGACAAAG 
GAGTGGCAAC TGGAGCTTCC CAAGCTGCTC ATCTCTGTTC ACGGAGGACT TCAAAACTTT 
GAGTTGCAGC CCAAACTAAA GCAAGTCTTC GGCAAAGGTC TTATAAAGGC TGCAATGACT 
ACCGGAGCCT GGATCTTCAC TGGAGGAGTT AATACTGGAG TCATCAGACA TGTTGGTGAT 
GCTCTGAAAG ATCATGCTTC CAAATCAAGA GGGAAGATCT GCACTATCGG CATTGCCCCC 
TGGGGGATTG TGGAGAACCA GGAAGACCTG GTGGGCAAAG ATGTGGTCCG GCCATACCAG 
ACGATGTCTA ACCCTCTTAG CAAGCTAACA GTACTCAACA GCCTACACTC ACACTTCATT 
TTGGCTGATA ATGGCACCAC CGGAAAGTAC GGAGCAGAAG TCAAACTGCG CAGGCAGCTG 
GAGAAGCACA TCTCTCTGCA AAAGATTAAC ACACGGATTG GACAGGGTGT TCCAGTAGTG 
GCTCTGATAG TGGAGGGGGG TCCGAATGTC ATCTCTATCG TCCTGGAGTA CCTAAGAGAC 
ACCCCTCCGG TGCCTGTTGT GGTGTGTGAC GGCAGCGGTC GAGCCTCTGA CATCCTGGCC 
TTTGGGCACA AGTACTCAGA GGAGGGCGGA ATCATAAACG AATCTTTGAG AGACCAGTTA 
TTAGTCACCA TTCAGAAGAC GTTCACATAC AGCCGGACGC AGGCCCAGCA TCTCTTCATC 
ATTCTGATGG AGTGCATGAA GAAGAAAGAA CTGATCACAG TGTTTCGGAT GGGATCAGAG 
GGACACCAGG ACATCGATCT AGCCATTCTA ACAGCTCTTC TGAAAGGAGC GAATGCATCA 
GCTCCAGATC AGCTAAGTCT GGCTTTGGCC TGGAACAGAG TTGACATCGC CCGGAGCCAG 
ATCTTCATTT ACGGACAGCA GTGGCCTGTT GGCTCTCTGG AGCAGTCTAT GCTGGACGCG 
CTGGTGCTGG ACCGAGTGGA CTTTGTAAAG CTGTTGATTG AGAACGGAGT GAGCATGCAC 
CGTTTCCTGA CACTCTCCAG ACTTGAAGAG CTTTATAATA CGAGGCACGG ACCATCCAAC 
ACCTTGTACC ACCTTGTCAG AGATGTGAAA AAGGGTAACC TGCCCCCCGA CTACCGCATT 
AGCCTGATTG ACATCGGATT GGTTATTGAA TACCTCATGG GTGGAGCCTA TCGCTGCAAT 
TACACCAGGA AGAGATTTCG GACCCTCTAT CACAACCTCT TTGGACCCAA AAGACCAAAA 
GCCCTGAAGC TGCTTGGAAT GGAGGATGAC ATGCCCATTA GACGAGGGCG TCAGAAGACT 
ACGCGGAAGC GAGAGGAGGA AGTAGATATT GACCTGGATG ACCCAGAAAT CAATCATTTC 
CCTTTCCCAT TCCATGAGCT GATGGTTTGG GCTGTGCTGA TGAAACGGCA GAAGATGGCA 
CTGTTCTTCT GGCAGCATGG AGAGGAGGCC ATGGCTAAAG CGCTGGTGGC CTGCAAGCTC 
TGTAAGGCAA TGGCTCACGA AGCTTCTGAG AACGACATGG TCGATGACAT TTCACAGGAG 
CTCAATCAAA ACTCAAGGGA GTTTGGTCAG CTGGCTGTTG AGCTGCTCGA TCAGTCCTAT 
AAGCAGGATG AACAGATGGC TATGAAGCTG TTGACCTACG AGCTGAAGAA CTGGAGTAAT 
GCCACCTGTC TGCAGCTGGC AGTGGCTGCC AAGCACCGAG ACTTCATCGC CCACACCTGT 
AGCCAGATGC TGCTCACAGA CATGTGGATG GGACGTCTGC GCATGCGCAA GAACTCTGGC 
CTGAAGGTTA TCTTGGGCCT GCTTCTCCCC CCTTCCATAT TGAGTCTGGA GTTTAAAAAT 
AAAGACGAGA TGTCATACAT GCCTCAGGAT CAGGACACAT ATCTGCAGGA GAAGGATGTG 
GATGAGCCCG AGAAACAAGC TAAGGAGAAA GAGGAGGAGG ACATGGAGTT CACAGTAAGA 
TCTTACTGTG AGACGCAGTA CAACTCCGTG GCTATGTTGG GAAACGTCAG TTCAGAAGCA 
TCGCGCAAGA AACAGGTGGA GGAGGTTCAG AACCGGCATC GGCTCATTCC ATTGGGTCGC 
AAAATATATG AGTTTTACAA CGCCCCTATC GTCAAGTTCT GGTTTCACAC GCTGGCGTAT 
GTAGGATACT TGATGCTCTT CAACTATATT GTGCTGGTGA AGATGGACCT GTGGCCTTCT 
CCTCAGGAGT GGATTGTTAT TGCCTACATA TTCACCAATG GCATCGAGAA GATGAGAGAG 
ATCCTGATGT CCGAACCTGG GAAGCTGCTT CAGAAGGTGA AAGTGTGGTT ACAGGAGTAC 
TGGAACATCA CTGACCTCAT GGCCATCCTC ATCTTCTCCA TCGGGATGGT GCTTCGTCTG 
CAGGACCCGC CGCTGATGAG CTACGGCAGG GTCATTTACT GCGTCAATAT CATTTATTGG 
TACATACGAC TCCTCGACAT CTTCGGAGTG AACAAATACC TGGGTCCATA CGTCATGATG 
ATCGGCAAGA TGATGATTGA CATGATGTAT TTTGTGATCA TCATGTTGGT GGTGCTGATG 
AGTTTCGGTG TGGCGCGTCA GGCCATCCTT AATCCCAATG AAGACCCATC CTGGATGCTG 
GCACGCAATA TCTTCTTCAT GCCATACTGG ATGATCTACG GAGAGGTGTT TGCTGACCAA 
ATCGACCCTC CTTGTGGTCA GAACATCACC ACAGAAGAAG GAGTTGTCAT GCCTCTTCCA 
CCCTGCAAAA CTGGAGCTTG GATTGTCCCT GCCATAATGG CCTGCTACCT GCTGGTGGCC 
AACATCCTGC TAGTTAACTT GCTCATTGCT GTCTTTAATA ACACTTTCTT TGAAGTCAAG 
TCCATTTCCA ACCAGGTGTG GAAGTTTCAG AGGTATCAGC TGATCATGAC CTTCCATGAA 
CGCCCGGTCC TTCCTCCTCC ACTCATCATC TTCAGCCACA TAACTATGGT TCTCAAACAC 
CTGTGCTGTC GCTGGAGGAA GCACGATGAG GATGAACGGG ACTATGGGCT GAAGCTTTTC 
ATCACTGAAG ATGAACTGAA GAAAGTGCAT GACTTTGAAG AGCAGTGCAT GGAGGAGTAT 
TTCAGAGAGA AAGATGACCG CTTCAATTCC TCCAATGACG AAAGGATACG TGTGACATCT 
GAAAGGGTGG AGAACATGGC GATGCGATTA GAGGAGGTGA ATGAGAGGGA ACATTTTATG 
AAAGCGTCCT TGCAGACTGT AGACATCCGG CTCGCCCAGA TGGAGGAGAT GATCGGCCGC 
ATTGCTGTGG CCCTAGAGAG AGTTGCTGGA ATGGACAGAG GCGAAGTTAA CAAAGCCCGG 
TCCAGGACAT CATCAGACTG CACTGATACC AATTACATCC TGCGCCAAAG CAGTTTCAAC 
AGCCAAGAAG GCAATTCCTA CAGGCTACAG GAGTCTCTGG AGCAGGGGGG CGACGAGTCC 
ATTTCTCCTA CTTCCCCAAC CGCCCTGGCT CCCCGTGTCC GCAGTCACTC CTTCTACGTG 
AGCCACTCTT CCAAAGACAG GAGTGGGGCG GACCGTGGAG AGGGCTTCTT TAAAGACAAG 
TTGTTCAGCC TCCACCGGGC CAACAGCTCA CAGTCTGTGT CCTCAGGAGC AGGTCCCAAA 
GAGTCCAAGC CAACCCCTTT GAATACTTTA TCCGTGCAGC AGCAGCTCCG CCCTTCTTCC 
TGCATAGACA TCTATGTTTC TGCCTCAGAG GACGTCCCGC CCACTGAGAG CTTCTTGGAT 
TCGGTTCGGA CAGTTCCAAC TCTGGCTCGA GACTCATCTC TTCATTCGGA GATCATGGAG 
GCGGTGCTCT CAGGGGGACG GGACTGTAGT GGAAGGGCAG GCGGCAGCGA AAGACAGTCG 
GATGGTACAG TCCTGTTTGA GGACAGCGCC GCCGCTGACC TATCGCTCTG CTCTGCCCAC 
CTGCTGCCGG ATTCCTTGCC CCCCTGGGAC CTGGACCCCT CACCGCCACC ATCAGCTGGT 
GTACTAGAGC GGTCCAAAAG TAGTCGCTTC CTGTCTACAG CAGGGCCTCT GTTTCTGGAC 
GAGCCTCATT TGGTTAAGTC ACACAGTCTG ATGTTCACAT CACGTGGGTA CTATGGTGGC 
ATGGGTGTGC AGGTGAAGGC GGCCGAATAC ACCAGCATCA CAGATTGCAT CGACACCCGT 
TGCGTCTCAA CCCCTTACCC TGTGCCAGAG CGGTCAGACT CACCTGGAGG ATCTTTCACC 
TTCGATAAGC CTCAAGACCT TGGCGTCTCG CACCCTGAGA GAGACGCTGA GCTCAGCCAC 
GCTGAGTCTG ACTTAGAGGA GCCAGCAGAG GGTTCGGGAG ACACATGTAA GGTGGGTCAG 
AGTAGCAGTA GTGGGGTAAT AGGTGCAGAC CTGGGCCTGG GCCTCGCACT AGGCCCGTTT 
TGCTCACCCA TCTCTAGACT AGAGCGAGCA AACAGCTGCT CCTCTTCAGA GGAGTCGCAC 
TCCAACATCT ACTCTCGAAA GAGCTTCTCA ATAAGCGAGA GAATGGACAA GGGCCGGGGG 
AGCTCCAGGA ACCCTTTCCA GAAGGCCAGA ACCGGGGCGA GGCTGGAGGG TAAGACAGAC 
TCACTGTCTA TGAGGAAAAT GGCCAAACCG TCAGCTTTCA GAAGTTTTGA CAGCAGACAC 
AACTACACGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	trpm3
Entrez Gene ID	565807
Full Name	transient receptor potential cation channel, subfamily M, member 3
Synonyms	si:dkey-201c13.4
General protein information	Preferred Names transient receptor potential cation channel, subfamily M, member 3 Names transient receptor potential cation channel subfamily M member 3 transient receptor potential melastatin 3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Homo sapiens (human)	TRPM3	NP_001007472.2
Mus musculus (house mouse)	Trpm3	NP_001030317.1
	TRPM3	XP_002800180.1
Canis lupus familiaris (dog)	TRPM3	XP_005615919.1
Xenopus tropicalis (tropical clawed frog)	trpm3	XP_004910826.1
Danio rerio (zebrafish)	trpm3	XP_005171986.1
Rattus norvegicus (Norway rat)	Trpm3	NP_001178491.1
Gallus gallus (chicken)	TRPM3	XP_004949217.1
Drosophila melanogaster (fruit fly)	trpm	NP_001137673.1
Pan troglodytes (chimpanzee)	TRPM3	XP_003951505.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006825	XP_308919.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

trpm3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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