edc4 cDNA ORF clone, Danio rerio(zebrafish)

The following edc4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the edc4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa149871 XM_021467676.1
Latest version!
Danio rerio enhancer of mRNA decapping 4 (edc4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa149872 XM_021467677.1
Latest version!
Danio rerio enhancer of mRNA decapping 4 (edc4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa38041 NM_001045085.1
Latest version!
Danio rerio enhancer of mRNA decapping 4 (edc4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa44697 XM_009293574.2
Latest version!
Danio rerio enhancer of mRNA decapping 4 (edc4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa44698 XM_009293575.2
Latest version!
Danio rerio enhancer of mRNA decapping 4 (edc4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa44699 XM_009293576.2
Latest version!
Danio rerio enhancer of mRNA decapping 4 (edc4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa149871
    Clone ID Related Accession (Same CDS sequence) XM_021467676.1
    Accession Version XM_021467676.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product enhancer of mRNA-decapping protein 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTTCCA GTTCCAGCAT GGACATAGAG GGTGCGACCC AGCACCTTCG GGACATCCTA 
    AAACTGGATC GTCCGGGAAA TTCGGCCGAA GCGTCTTTGG TCGAGAGCCA GAGAAAACCA
    TCTTTCAATG GAGAGCTGAA CGGAGTTCTC GGATCCGATC TCATGGGAGC AGCAATGGTG
    GAGCCGACGG TGCACAACTC AGACAAACTT AATGGACAGG ACACACAGAC CATCTGTCTT
    TCAGGAGATG ACGGGTCCAT TTGTGTCCCA ATCAGAGCGA ATATTGTGGA AATTGTGTCC
    AGCCGTGACT CTAGTATTGA CAGTAAGGCT CGTGGAAGCA ACAAGGTTAA AATTCAGCCA
    GTGGCCAAAT ATGACTGGGA GCACAAGTAC TACTATGGCA ATTTAATAGC CGTGTCCAAC
    ACGTACTTGG CTTATGCTAT CAGAGGTGCA AACAATCATT CAATGATTCG TGTGCTGCAC
    CTGAGCTCTA CAGAGCGCTC TCTGCTGAAG GGCTTCACCG GGGCTGTCAC AGACCTTGCC
    TTTGCCCATC TGGACTCCAC CCTGTTGGGA TGTGTGGACG AAGCCGGAAA CATGTTCATT
    TGGCAGCTTA CAAGCCACAG CAGCAAAATA CAAGACGAAG TGATTGTTCA CATCCGCAGA
    CCTGAGGACA CTCCACTAAA CTCTAACCGC AGACTCATCT GGTGTCCCTT CATCCCGGAG
    GACAATGACG AGAGCCCAGA GGATGCATGT CAGACATTAG CATTGCTTCA TGAAGACAGA
    GCTGAGGTTT GGGACCTCGA CATCCTCCGA TCAAATAACA GCTCATGGCC TGTGGACGCC
    ACTGAATTGA AAGAGGGTTT CATCACCATT AGAGGGCATG CAGCTCGCAT AAGTGAGGGA
    GCTCTGTCCC CTGATGGTAC GGTTTTGGCC ACAGCTAGTC ATGATGGATA TGTCAAATTT
    TGGCAAATTT ACATTGAAGG ACAGGACCAA CCCCGGTCAT TCAAGACTTT GTCGCAAGAA
    ATTGCAAGAT GCCTGCATGA ATGGCAGCCG CATAATGGAC AGCCTCTATC TTGCCTGCTG
    TTTTGTGACA ACCACAAGAA GCAAGATCCA GACGTTCCAT TCTGGCGCTT CCTGATTACT
    GGAGCTGATC AGAACCAGGA GCTGAAGATG TGGTGCACAG TATCATGGAC CTGCCTACAA
    ACCATCAGGT TTTCTCCAGA TCCATTTAAC TGCGGTGTGC TACCCAGCCT GAAGGCCAGT
    TTGGACCTTT CTGCAGAATT TCTCATTCTT AGTGATGTTC AAAGGAAGGT ATTGTATGTC
    ATGGAGCTGC TGCAGGATCA AGAGAAAGGC CGTGCCAGTT TCACTGCAGT CTCAGAGTTT
    CTGCTCACAC ACCCTGTGCT CAGCTTCGGT GTTCAGGATG TTTCTCGTGC TCGCCTGCGC
    CATACTGAGG TCCTTCCTCC TGAAGAAGAA AGCGAAAGCA TGACAGCAGA AGGAAACCAG
    GGAACCTCTG AGTCCAGATC AGGAATTCAG ATTAAACTCT ACTGTGTGCA TACAAAATCA
    CTACAGGATG TGCAGATCTG GTTTCAGCCC AATCAGGGGG ACTCCTCTTC ACTCTTCATG
    CCTCAGTCTG GATCACAGGA TGGGTTCAGT TTTTCTGACC CGCTGGCTGA TCTGAATGTG
    GAGGCAATGA GCTCTGACAA GGAGTCTGGC GATAGTGGTT CACAAAATGA CCTGAGTAAG
    ATTCTGCCTC TTCCAAGTCC CGCTGACTTC ATGAGTCCAG CCCCATCAGC CTTGCCCAAA
    CTCATGACCC CGGATGCCTT CATGACACCC AGCGCCTCTA TGCCCGCTTC TCCTGGCAGC
    AGTGCTAGCA GTCTGACCAT TGTCACGGCC ATGAGCAGCT CAGACAGCGG TGCAAGAGGT
    GGAGATGATC TGACTCAGAG CCCTAAAATG TCTGTTGAAT GTGTCAACAG CAGTTTCGCC
    AATGCAGGCA GTCCAAGATC CAACTCCATC CTCATCTCAG GCCTTGGGGA AAACATCCAG
    GCTTTCGCTC CCCCACTGGT TCTTTCTTCA TCCCTCCTTC AAGAAGCCTC TCTGCTCTGC
    GAAGCTGCTC CTTATTCTCA CCGACTCCCC CTCCCTTATT TCCCATCTCC TCCTCCGCAG
    GTTTCCCCTC CAAACCCTCC TCTCTCTCTG GATCTCCAGG CAATAGATCC CATGGTTGTC
    CCACAGGCGT CTCCAACAAG AGCTCGCTCA CCGGATGTCA TCTCATCTGC CTCTACTGCA
    ATGTCCCAGG ACATACCTGA GATTGCCTCC GAGACGCTGC AGAGGGGACT GTCCGGTGCC
    AATGCTGATT CGGGCCCCAT CCTGCACTCT GACAGCATGG CATCAGCCGC ATCAATTCTC
    CACCTGCTAT CTCCAAGGGC CCGGAGCAGC GCTGAGCACA GTTTGTTGCC TCTTGAATTG
    GGTGCAGCGT CTGTGGATGG AGAGCAGAGA CTCAGTAACA CACCATCCCT GCTGGAGACT
    GCTCTCTCAC AGGAAAACGC CGGAGCAGCC GGAGGATCCT GTTCTGACAG CAGTGTGAAC
    CACGCATGGC CTGCTGCTCC TGATATCACG CGAGAGACGC GCAACAGCCT GCGAGACAAC
    GGTTTAGGAG ACTGCTCTAG GGAGGAAATA AAAGACAGGC ACATTTCCTC CCCCTACCAT
    AGACGCACAT ATCACCTGAC TCAGAACGAC AGTCAAGATG CAAGTGCAGA GCAGAGTGAT
    CATGACGATG AGGTTGCCAG TCTGGCCTCG TCCTCAGGGA ACTGCGGCCC TCGCTCCTCT
    CACAGACTTC CTGTAAAGGA CTGGAAAACA TCACCCCGCA GCTCCCCTAA GCTCAAGCGG
    AAGAGCAAGA AAGATGAAGG AGAGTCCTCG CAATCTAGAC AGATTGAAAG CCAGATGAGC
    ACAGAGGTGC AGGACGAGCT CCTGCAGATG CTGCGGAGCC AGCAGAGGGA GATCGCAGAG
    CTCCGACAGA ATCAGCTTGA TCTTCTGCAG AGAGTCACTA GCCACATGGA CGCAGTGCAG
    AGCTCCATCA TGGCTCACAT CGAGCATGCC ATGTTAGCTC AGCAAGAACA AGAACAGAGA
    AGAATGGAGC GAATTCTGGT CGAGGGTCAA AGTCGAAACC AACAGCTCCA GGACCAGCTA
    GTTCAGCAGC TCGTCCAAAC TCTGAACAAC AGTCTCTGCA ACCGGCTTGA GAAAGTTTTG
    CGTGAAGAAA TGAAGAAAAC CGTCCCGCAG ACCATATCGA AGAGTCTCGA ACCTGTGACC
    GGCCAGATGA ACAGCACCAT TGCTGCCAAG TTAACTGCAG TAGAAGGAGC ACTCAAAGAA
    AACGTCACCA AAGTTGTTAA GTCCAAGAAC ACTACAGATG CCATTGGTCG AGCTGCTGCA
    GAGGCCATGC AGGGTCCTAT TCAGGCAGCC TACAAAGAGA CCTTCCAGAG CATTGTCCTG
    CCTGTGTTTG AGAGAGGCTG TCAGTCCATG TTTCAGCAGA TCAATGACAG CTTCAAACAG
    GGAACCAACG AATACATCCA GCAGTTGGAG ACGCACATCA AAAACAGGAA ACAAAGAGAC
    CAGGATACAC GGGATCCTGT CATTGGCCAG TTACAGCAGA TGATTGACAG TCTTCAGAGT
    TCACAAGACC AGCTGGCGTC TACAGTGACG GCCAGCGTTA GCTCTGACGT CCAGCATCAG
    CTCCACATGA TTGTCGGAAA TCTGCAGGAC TCAATCCTGA CACAGGTGCA GCGTATAGTG
    AAGGGAGAGG TGAGTTTGGC TATGAAGGAG CAGCAGGCCG CCGTCACCTC CAGTATCATG
    CAGGCCATGC GCTCAGCCGC AGGAACACCC GTCCCATCCG CACATCTGGA CTACCAGGCC
    CAGCAGGCCA GCATATTACA GCTACTACAG CAGGGCCAGC TTAACGAGGC CTTCCAGCAG
    GCTTTATCCG CAGCAGACCT GAATCTGGTC CTGTACGTGT GCGAGACCAT CGACTCCCAA
    CAAGTGTTCG GCCAGCAGCC CTGTCCACTC CACCAGGCCG TCCTTCTGTC CCTCATCCAG
    CAGCTCTCCA CCAACCTGAG CACGCGCACA GAACTCAAGA TCAGCTATCT CGAAGATGCT
    GTGATGAACC TTGACCACGG AGATCCCGTC ACCCGAGACC ACATGTCCGC AGTGCTGACC
    CAGGTACGAC AGAAGCTCTT CCTGTTCCTG CAGCAGGACG CCCACAGCCC TATGAGCAAG
    CGTGCACGGC GCCTCATGAT GATGCTGCAG GGCCTGGTCA ACCACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323351.1
    CDS132..4439
    Translation

    Target ORF information:

    RefSeq Version XM_021467676.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio enhancer of mRNA decapping 4 (edc4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467676.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTTCCA GTTCCAGCAT GGACATAGAG GGTGCGACCC AGCACCTTCG GGACATCCTA 
    AAACTGGATC GTCCGGGAAA TTCGGCCGAA GCGTCTTTGG TCGAGAGCCA GAGAAAACCA
    TCTTTCAATG GAGAGCTGAA CGGAGTTCTC GGATCCGATC TCATGGGAGC AGCAATGGTG
    GAGCCGACGG TGCACAACTC AGACAAACTT AATGGACAGG ACACACAGAC CATCTGTCTT
    TCAGGAGATG ACGGGTCCAT TTGTGTCCCA ATCAGAGCGA ATATTGTGGA AATTGTGTCC
    AGCCGTGACT CTAGTATTGA CAGTAAGGCT CGTGGAAGCA ACAAGGTTAA AATTCAGCCA
    GTGGCCAAAT ATGACTGGGA GCACAAGTAC TACTATGGCA ATTTAATAGC CGTGTCCAAC
    ACGTACTTGG CTTATGCTAT CAGAGGTGCA AACAATCATT CAATGATTCG TGTGCTGCAC
    CTGAGCTCTA CAGAGCGCTC TCTGCTGAAG GGCTTCACCG GGGCTGTCAC AGACCTTGCC
    TTTGCCCATC TGGACTCCAC CCTGTTGGGA TGTGTGGACG AAGCCGGAAA CATGTTCATT
    TGGCAGCTTA CAAGCCACAG CAGCAAAATA CAAGACGAAG TGATTGTTCA CATCCGCAGA
    CCTGAGGACA CTCCACTAAA CTCTAACCGC AGACTCATCT GGTGTCCCTT CATCCCGGAG
    GACAATGACG AGAGCCCAGA GGATGCATGT CAGACATTAG CATTGCTTCA TGAAGACAGA
    GCTGAGGTTT GGGACCTCGA CATCCTCCGA TCAAATAACA GCTCATGGCC TGTGGACGCC
    ACTGAATTGA AAGAGGGTTT CATCACCATT AGAGGGCATG CAGCTCGCAT AAGTGAGGGA
    GCTCTGTCCC CTGATGGTAC GGTTTTGGCC ACAGCTAGTC ATGATGGATA TGTCAAATTT
    TGGCAAATTT ACATTGAAGG ACAGGACCAA CCCCGGTCAT TCAAGACTTT GTCGCAAGAA
    ATTGCAAGAT GCCTGCATGA ATGGCAGCCG CATAATGGAC AGCCTCTATC TTGCCTGCTG
    TTTTGTGACA ACCACAAGAA GCAAGATCCA GACGTTCCAT TCTGGCGCTT CCTGATTACT
    GGAGCTGATC AGAACCAGGA GCTGAAGATG TGGTGCACAG TATCATGGAC CTGCCTACAA
    ACCATCAGGT TTTCTCCAGA TCCATTTAAC TGCGGTGTGC TACCCAGCCT GAAGGCCAGT
    TTGGACCTTT CTGCAGAATT TCTCATTCTT AGTGATGTTC AAAGGAAGGT ATTGTATGTC
    ATGGAGCTGC TGCAGGATCA AGAGAAAGGC CGTGCCAGTT TCACTGCAGT CTCAGAGTTT
    CTGCTCACAC ACCCTGTGCT CAGCTTCGGT GTTCAGGATG TTTCTCGTGC TCGCCTGCGC
    CATACTGAGG TCCTTCCTCC TGAAGAAGAA AGCGAAAGCA TGACAGCAGA AGGAAACCAG
    GGAACCTCTG AGTCCAGATC AGGAATTCAG ATTAAACTCT ACTGTGTGCA TACAAAATCA
    CTACAGGATG TGCAGATCTG GTTTCAGCCC AATCAGGGGG ACTCCTCTTC ACTCTTCATG
    CCTCAGTCTG GATCACAGGA TGGGTTCAGT TTTTCTGACC CGCTGGCTGA TCTGAATGTG
    GAGGCAATGA GCTCTGACAA GGAGTCTGGC GATAGTGGTT CACAAAATGA CCTGAGTAAG
    ATTCTGCCTC TTCCAAGTCC CGCTGACTTC ATGAGTCCAG CCCCATCAGC CTTGCCCAAA
    CTCATGACCC CGGATGCCTT CATGACACCC AGCGCCTCTA TGCCCGCTTC TCCTGGCAGC
    AGTGCTAGCA GTCTGACCAT TGTCACGGCC ATGAGCAGCT CAGACAGCGG TGCAAGAGGT
    GGAGATGATC TGACTCAGAG CCCTAAAATG TCTGTTGAAT GTGTCAACAG CAGTTTCGCC
    AATGCAGGCA GTCCAAGATC CAACTCCATC CTCATCTCAG GCCTTGGGGA AAACATCCAG
    GCTTTCGCTC CCCCACTGGT TCTTTCTTCA TCCCTCCTTC AAGAAGCCTC TCTGCTCTGC
    GAAGCTGCTC CTTATTCTCA CCGACTCCCC CTCCCTTATT TCCCATCTCC TCCTCCGCAG
    GTTTCCCCTC CAAACCCTCC TCTCTCTCTG GATCTCCAGG CAATAGATCC CATGGTTGTC
    CCACAGGCGT CTCCAACAAG AGCTCGCTCA CCGGATGTCA TCTCATCTGC CTCTACTGCA
    ATGTCCCAGG ACATACCTGA GATTGCCTCC GAGACGCTGC AGAGGGGACT GTCCGGTGCC
    AATGCTGATT CGGGCCCCAT CCTGCACTCT GACAGCATGG CATCAGCCGC ATCAATTCTC
    CACCTGCTAT CTCCAAGGGC CCGGAGCAGC GCTGAGCACA GTTTGTTGCC TCTTGAATTG
    GGTGCAGCGT CTGTGGATGG AGAGCAGAGA CTCAGTAACA CACCATCCCT GCTGGAGACT
    GCTCTCTCAC AGGAAAACGC CGGAGCAGCC GGAGGATCCT GTTCTGACAG CAGTGTGAAC
    CACGCATGGC CTGCTGCTCC TGATATCACG CGAGAGACGC GCAACAGCCT GCGAGACAAC
    GGTTTAGGAG ACTGCTCTAG GGAGGAAATA AAAGACAGGC ACATTTCCTC CCCCTACCAT
    AGACGCACAT ATCACCTGAC TCAGAACGAC AGTCAAGATG CAAGTGCAGA GCAGAGTGAT
    CATGACGATG AGGTTGCCAG TCTGGCCTCG TCCTCAGGGA ACTGCGGCCC TCGCTCCTCT
    CACAGACTTC CTGTAAAGGA CTGGAAAACA TCACCCCGCA GCTCCCCTAA GCTCAAGCGG
    AAGAGCAAGA AAGATGAAGG AGAGTCCTCG CAATCTAGAC AGATTGAAAG CCAGATGAGC
    ACAGAGGTGC AGGACGAGCT CCTGCAGATG CTGCGGAGCC AGCAGAGGGA GATCGCAGAG
    CTCCGACAGA ATCAGCTTGA TCTTCTGCAG AGAGTCACTA GCCACATGGA CGCAGTGCAG
    AGCTCCATCA TGGCTCACAT CGAGCATGCC ATGTTAGCTC AGCAAGAACA AGAACAGAGA
    AGAATGGAGC GAATTCTGGT CGAGGGTCAA AGTCGAAACC AACAGCTCCA GGACCAGCTA
    GTTCAGCAGC TCGTCCAAAC TCTGAACAAC AGTCTCTGCA ACCGGCTTGA GAAAGTTTTG
    CGTGAAGAAA TGAAGAAAAC CGTCCCGCAG ACCATATCGA AGAGTCTCGA ACCTGTGACC
    GGCCAGATGA ACAGCACCAT TGCTGCCAAG TTAACTGCAG TAGAAGGAGC ACTCAAAGAA
    AACGTCACCA AAGTTGTTAA GTCCAAGAAC ACTACAGATG CCATTGGTCG AGCTGCTGCA
    GAGGCCATGC AGGGTCCTAT TCAGGCAGCC TACAAAGAGA CCTTCCAGAG CATTGTCCTG
    CCTGTGTTTG AGAGAGGCTG TCAGTCCATG TTTCAGCAGA TCAATGACAG CTTCAAACAG
    GGAACCAACG AATACATCCA GCAGTTGGAG ACGCACATCA AAAACAGGAA ACAAAGAGAC
    CAGGATACAC GGGATCCTGT CATTGGCCAG TTACAGCAGA TGATTGACAG TCTTCAGAGT
    TCACAAGACC AGCTGGCGTC TACAGTGACG GCCAGCGTTA GCTCTGACGT CCAGCATCAG
    CTCCACATGA TTGTCGGAAA TCTGCAGGAC TCAATCCTGA CACAGGTGCA GCGTATAGTG
    AAGGGAGAGG TGAGTTTGGC TATGAAGGAG CAGCAGGCCG CCGTCACCTC CAGTATCATG
    CAGGCCATGC GCTCAGCCGC AGGAACACCC GTCCCATCCG CACATCTGGA CTACCAGGCC
    CAGCAGGCCA GCATATTACA GCTACTACAG CAGGGCCAGC TTAACGAGGC CTTCCAGCAG
    GCTTTATCCG CAGCAGACCT GAATCTGGTC CTGTACGTGT GCGAGACCAT CGACTCCCAA
    CAAGTGTTCG GCCAGCAGCC CTGTCCACTC CACCAGGCCG TCCTTCTGTC CCTCATCCAG
    CAGCTCTCCA CCAACCTGAG CACGCGCACA GAACTCAAGA TCAGCTATCT CGAAGATGCT
    GTGATGAACC TTGACCACGG AGATCCCGTC ACCCGAGACC ACATGTCCGC AGTGCTGACC
    CAGGTACGAC AGAAGCTCTT CCTGTTCCTG CAGCAGGACG CCCACAGCCC TATGAGCAAG
    CGTGCACGGC GCCTCATGAT GATGCTGCAG GGCCTGGTCA ACCACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149872
    Clone ID Related Accession (Same CDS sequence) XM_021467677.1
    Accession Version XM_021467677.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4188bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product enhancer of mRNA-decapping protein 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCTTCCA GTTCCAGCAT GGACATAGAG GGTGCGACCC AGCACCTTCG GGACATCCTA 
    AAACTGGATC GTCCGGGAAA TTCGGCCGAA GCGTCTTTGG TCGAGAGCCA GAGAAAACCA
    TCTTTCAATG GAGAGCTGAA CGGAGTTCTC GGATCCGATC TCATGGGAGC AGCAATGGTG
    GAGCCGACGG TGCACAACTC AGACAAACTT AATGGACAGG ACACACAGAC CATCTGTCTT
    TCAGGAGATG ACGGGTCCAT TTGTGTCCCA ATCAGAGCGA ATATTGTGGA AATTGTGTCC
    AGCCGTGACT CTAGTATTGA CAGTAAGGCT CGTGGAAGCA ACAAGGTTAA AATTCAGCCA
    GTGGCCAAAT ATGACTGGGA GCACAAGTAC TACTATGGCA ATTTAATAGC CGTGTCCAAC
    ACGTACTTGG CTTATGCTAT CAGAGGTGCA AACAATCATT CAATGATTCG TGTGCTGCAC
    CTGAGCTCTA CAGAGCGCTC TCTGCTGAAG GGCTTCACCG GGGCTGTCAC AGACCTTGCC
    TTTGCCCATC TGGACTCCAC CCTGTTGGGA TGTGTGGACG AAGCCGGAAA CATGTTCATT
    TGGCAGCTTA CAAGCCACAG CAGCAAAATA CAAGACGAAG TGATTGTTCA CATCCGCAGA
    CCTGAGGACA CTCCACTAAA CTCTAACCGC AGACTCATCT GGTGTCCCTT CATCCCGGAG
    GACAATGACG AGAGCCCAGA GGATGCATGT CAGACATTAG CATTGCTTCA TGAAGACAGA
    GCTGAGGTTT GGGACCTCGA CATCCTCCGA TCAAATAACA GCTCATGGCC TGTGGACGCC
    ACTGAATTGA AAGAGGGTTT CATCACCATT AGAGGGCATG CAGCTCGCAT AAGTGAGGGA
    GCTCTGTCCC CTGATGGTAC GGTTTTGGCC ACAGCTAGTC ATGATGGATA TGTCAAATTT
    TGGCAAATTT ACATTGAAGG ACAGGACCAA CCCCGGTCAT TCAAGACTTT GTCGCAAGAA
    ATTGCAAGAT GCCTGCATGA ATGGCAGCCG CATAATGGAC AGCCTCTATC TTGCCTGCTG
    TTTTGTGACA ACCACAAGAA GCAAGATCCA GACGTTCCAT TCTGGCGCTT CCTGATTACT
    GGAGCTGATC AGAACCAGGA GCTGAAGATG TGGTGCACAG TATCATGGAC CTGCCTACAA
    ACCATCAGGT TTTCTCCAGA TCCATTTAAC TGCGGTGTGC TACCCAGCCT GAAGGCCAGT
    TTGGACCTTT CTGCAGAATT TCTCATTCTT AGTGATGTTC AAAGGAAGGT ATTGTATGTC
    ATGGAGCTGC TGCAGGATCA AGAGAAAGGC CGTGCCAGTT TCACTGCAGT CTCAGAGTTT
    CTGCTCACAC ACCCTGTGCT CAGCTTCGGT GTTCAGGATG TTTCTCGTGC TCGCCTGCGC
    CATACTGAGG TCCTTCCTCC TGAAGAAGAA AGCGAAAGCA TGACAGCAGA AGGAAACCAG
    GGAACCTCTG AGTCCAGATC AGGAATTCAG ATTAAACTCT ACTGTGTGCA TACAAAATCA
    CTACAGGATG TGCAGATCTG GTTTCAGCCC AATCAGGGGG ACTCCTCTTC ACTCTTCATG
    CCTCAGTCTG GATCACAGGA TGGGTTCAGT TTTTCTGACC CGCTGGCTGA TCTGAATGTG
    GAGGCAATGA GCTCTGACAA GGAGTCTGGC GATAGTGGTT CACAAAATGA CCTGAGTAAG
    ATTCTGCCTC TTCCAAGTCC CGCTGACTTC ATGAGTCCAG CCCCATCAGC CTTGCCCAAA
    CTCATGACCC CGGATGCCTT CATGACACCC AGCGCCTCTA TGCCCGCTTC TCCTGGCAGC
    AGTGCTAGCA GTCTGACCAT TGTCACGGCC ATGAGCAGCT CAGACAGCGG TGCAAGAGGT
    GGAGATGATC TGACTCAGAG CCCTAAAATG TCTGTTGAAT GTGTCAACAG CAGTTTCGCC
    AATGCAGGCA GTCCAAGATC CAACTCCATC CTCATCTCAG GCCTTGGGGA AAACATCCAG
    GTTTCCCCTC CAAACCCTCC TCTCTCTCTG GATCTCCAGG CAATAGATCC CATGGTTGTC
    CCACAGGCGT CTCCAACAAG AGCTCGCTCA CCGGATGTCA TCTCATCTGC CTCTACTGCA
    ATGTCCCAGG ACATACCTGA GATTGCCTCC GAGACGCTGC AGAGGGGACT GTCCGGTGCC
    AATGCTGATT CGGGCCCCAT CCTGCACTCT GACAGCATGG CATCAGCCGC ATCAATTCTC
    CACCTGCTAT CTCCAAGGGC CCGGAGCAGC GCTGAGCACA GTTTGTTGCC TCTTGAATTG
    GGTGCAGCGT CTGTGGATGG AGAGCAGAGA CTCAGTAACA CACCATCCCT GCTGGAGACT
    GCTCTCTCAC AGGAAAACGC CGGAGCAGCC GGAGGATCCT GTTCTGACAG CAGTGTGAAC
    CACGCATGGC CTGCTGCTCC TGATATCACG CGAGAGACGC GCAACAGCCT GCGAGACAAC
    GGTTTAGGAG ACTGCTCTAG GGAGGAAATA AAAGACAGGC ACATTTCCTC CCCCTACCAT
    AGACGCACAT ATCACCTGAC TCAGAACGAC AGTCAAGATG CAAGTGCAGA GCAGAGTGAT
    CATGACGATG AGGTTGCCAG TCTGGCCTCG TCCTCAGGGA ACTGCGGCCC TCGCTCCTCT
    CACAGACTTC CTGTAAAGGA CTGGAAAACA TCACCCCGCA GCTCCCCTAA GCTCAAGCGG
    AAGAGCAAGA AAGATGAAGG AGAGTCCTCG CAATCTAGAC AGATTGAAAG CCAGATGAGC
    ACAGAGGTGC AGGACGAGCT CCTGCAGATG CTGCGGAGCC AGCAGAGGGA GATCGCAGAG
    CTCCGACAGA ATCAGCTTGA TCTTCTGCAG AGAGTCACTA GCCACATGGA CGCAGTGCAG
    AGCTCCATCA TGGCTCACAT CGAGCATGCC ATGTTAGCTC AGCAAGAACA AGAACAGAGA
    AGAATGGAGC GAATTCTGGT CGAGGGTCAA AGTCGAAACC AACAGCTCCA GGACCAGCTA
    GTTCAGCAGC TCGTCCAAAC TCTGAACAAC AGTCTCTGCA ACCGGCTTGA GAAAGTTTTG
    CGTGAAGAAA TGAAGAAAAC CGTCCCGCAG ACCATATCGA AGAGTCTCGA ACCTGTGACC
    GGCCAGATGA ACAGCACCAT TGCTGCCAAG TTAACTGCAG TAGAAGGAGC ACTCAAAGAA
    AACGTCACCA AAGTTGTTAA GTCCAAGAAC ACTACAGATG CCATTGGTCG AGCTGCTGCA
    GAGGCCATGC AGGGTCCTAT TCAGGCAGCC TACAAAGAGA CCTTCCAGAG CATTGTCCTG
    CCTGTGTTTG AGAGAGGCTG TCAGTCCATG TTTCAGCAGA TCAATGACAG CTTCAAACAG
    GGAACCAACG AATACATCCA GCAGTTGGAG ACGCACATCA AAAACAGGAA ACAAAGAGAC
    CAGGATACAC GGGATCCTGT CATTGGCCAG TTACAGCAGA TGATTGACAG TCTTCAGAGT
    TCACAAGACC AGCTGGCGTC TACAGTGACG GCCAGCGTTA GCTCTGACGT CCAGCATCAG
    CTCCACATGA TTGTCGGAAA TCTGCAGGAC TCAATCCTGA CACAGGTGCA GCGTATAGTG
    AAGGGAGAGG TGAGTTTGGC TATGAAGGAG CAGCAGGCCG CCGTCACCTC CAGTATCATG
    CAGGCCATGC GCTCAGCCGC AGGAACACCC GTCCCATCCG CACATCTGGA CTACCAGGCC
    CAGCAGGCCA GCATATTACA GCTACTACAG CAGGGCCAGC TTAACGAGGC CTTCCAGCAG
    GCTTTATCCG CAGCAGACCT GAATCTGGTC CTGTACGTGT GCGAGACCAT CGACTCCCAA
    CAAGTGTTCG GCCAGCAGCC CTGTCCACTC CACCAGGCCG TCCTTCTGTC CCTCATCCAG
    CAGCTCTCCA CCAACCTGAG CACGCGCACA GAACTCAAGA TCAGCTATCT CGAAGATGCT
    GTGATGAACC TTGACCACGG AGATCCCGTC ACCCGAGACC ACATGTCCGC AGTGCTGACC
    CAGGTACGAC AGAAGCTCTT CCTGTTCCTG CAGCAGGACG CCCACAGCCC TATGAGCAAG
    CGTGCACGGC GCCTCATGAT GATGCTGCAG GGCCTGGTCA ACCACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021323352.1
    CDS132..4319
    Translation

    Target ORF information:

    RefSeq Version XM_021467677.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio enhancer of mRNA decapping 4 (edc4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021467677.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCTTCCA GTTCCAGCAT GGACATAGAG GGTGCGACCC AGCACCTTCG GGACATCCTA 
    AAACTGGATC GTCCGGGAAA TTCGGCCGAA GCGTCTTTGG TCGAGAGCCA GAGAAAACCA
    TCTTTCAATG GAGAGCTGAA CGGAGTTCTC GGATCCGATC TCATGGGAGC AGCAATGGTG
    GAGCCGACGG TGCACAACTC AGACAAACTT AATGGACAGG ACACACAGAC CATCTGTCTT
    TCAGGAGATG ACGGGTCCAT TTGTGTCCCA ATCAGAGCGA ATATTGTGGA AATTGTGTCC
    AGCCGTGACT CTAGTATTGA CAGTAAGGCT CGTGGAAGCA ACAAGGTTAA AATTCAGCCA
    GTGGCCAAAT ATGACTGGGA GCACAAGTAC TACTATGGCA ATTTAATAGC CGTGTCCAAC
    ACGTACTTGG CTTATGCTAT CAGAGGTGCA AACAATCATT CAATGATTCG TGTGCTGCAC
    CTGAGCTCTA CAGAGCGCTC TCTGCTGAAG GGCTTCACCG GGGCTGTCAC AGACCTTGCC
    TTTGCCCATC TGGACTCCAC CCTGTTGGGA TGTGTGGACG AAGCCGGAAA CATGTTCATT
    TGGCAGCTTA CAAGCCACAG CAGCAAAATA CAAGACGAAG TGATTGTTCA CATCCGCAGA
    CCTGAGGACA CTCCACTAAA CTCTAACCGC AGACTCATCT GGTGTCCCTT CATCCCGGAG
    GACAATGACG AGAGCCCAGA GGATGCATGT CAGACATTAG CATTGCTTCA TGAAGACAGA
    GCTGAGGTTT GGGACCTCGA CATCCTCCGA TCAAATAACA GCTCATGGCC TGTGGACGCC
    ACTGAATTGA AAGAGGGTTT CATCACCATT AGAGGGCATG CAGCTCGCAT AAGTGAGGGA
    GCTCTGTCCC CTGATGGTAC GGTTTTGGCC ACAGCTAGTC ATGATGGATA TGTCAAATTT
    TGGCAAATTT ACATTGAAGG ACAGGACCAA CCCCGGTCAT TCAAGACTTT GTCGCAAGAA
    ATTGCAAGAT GCCTGCATGA ATGGCAGCCG CATAATGGAC AGCCTCTATC TTGCCTGCTG
    TTTTGTGACA ACCACAAGAA GCAAGATCCA GACGTTCCAT TCTGGCGCTT CCTGATTACT
    GGAGCTGATC AGAACCAGGA GCTGAAGATG TGGTGCACAG TATCATGGAC CTGCCTACAA
    ACCATCAGGT TTTCTCCAGA TCCATTTAAC TGCGGTGTGC TACCCAGCCT GAAGGCCAGT
    TTGGACCTTT CTGCAGAATT TCTCATTCTT AGTGATGTTC AAAGGAAGGT ATTGTATGTC
    ATGGAGCTGC TGCAGGATCA AGAGAAAGGC CGTGCCAGTT TCACTGCAGT CTCAGAGTTT
    CTGCTCACAC ACCCTGTGCT CAGCTTCGGT GTTCAGGATG TTTCTCGTGC TCGCCTGCGC
    CATACTGAGG TCCTTCCTCC TGAAGAAGAA AGCGAAAGCA TGACAGCAGA AGGAAACCAG
    GGAACCTCTG AGTCCAGATC AGGAATTCAG ATTAAACTCT ACTGTGTGCA TACAAAATCA
    CTACAGGATG TGCAGATCTG GTTTCAGCCC AATCAGGGGG ACTCCTCTTC ACTCTTCATG
    CCTCAGTCTG GATCACAGGA TGGGTTCAGT TTTTCTGACC CGCTGGCTGA TCTGAATGTG
    GAGGCAATGA GCTCTGACAA GGAGTCTGGC GATAGTGGTT CACAAAATGA CCTGAGTAAG
    ATTCTGCCTC TTCCAAGTCC CGCTGACTTC ATGAGTCCAG CCCCATCAGC CTTGCCCAAA
    CTCATGACCC CGGATGCCTT CATGACACCC AGCGCCTCTA TGCCCGCTTC TCCTGGCAGC
    AGTGCTAGCA GTCTGACCAT TGTCACGGCC ATGAGCAGCT CAGACAGCGG TGCAAGAGGT
    GGAGATGATC TGACTCAGAG CCCTAAAATG TCTGTTGAAT GTGTCAACAG CAGTTTCGCC
    AATGCAGGCA GTCCAAGATC CAACTCCATC CTCATCTCAG GCCTTGGGGA AAACATCCAG
    GTTTCCCCTC CAAACCCTCC TCTCTCTCTG GATCTCCAGG CAATAGATCC CATGGTTGTC
    CCACAGGCGT CTCCAACAAG AGCTCGCTCA CCGGATGTCA TCTCATCTGC CTCTACTGCA
    ATGTCCCAGG ACATACCTGA GATTGCCTCC GAGACGCTGC AGAGGGGACT GTCCGGTGCC
    AATGCTGATT CGGGCCCCAT CCTGCACTCT GACAGCATGG CATCAGCCGC ATCAATTCTC
    CACCTGCTAT CTCCAAGGGC CCGGAGCAGC GCTGAGCACA GTTTGTTGCC TCTTGAATTG
    GGTGCAGCGT CTGTGGATGG AGAGCAGAGA CTCAGTAACA CACCATCCCT GCTGGAGACT
    GCTCTCTCAC AGGAAAACGC CGGAGCAGCC GGAGGATCCT GTTCTGACAG CAGTGTGAAC
    CACGCATGGC CTGCTGCTCC TGATATCACG CGAGAGACGC GCAACAGCCT GCGAGACAAC
    GGTTTAGGAG ACTGCTCTAG GGAGGAAATA AAAGACAGGC ACATTTCCTC CCCCTACCAT
    AGACGCACAT ATCACCTGAC TCAGAACGAC AGTCAAGATG CAAGTGCAGA GCAGAGTGAT
    CATGACGATG AGGTTGCCAG TCTGGCCTCG TCCTCAGGGA ACTGCGGCCC TCGCTCCTCT
    CACAGACTTC CTGTAAAGGA CTGGAAAACA TCACCCCGCA GCTCCCCTAA GCTCAAGCGG
    AAGAGCAAGA AAGATGAAGG AGAGTCCTCG CAATCTAGAC AGATTGAAAG CCAGATGAGC
    ACAGAGGTGC AGGACGAGCT CCTGCAGATG CTGCGGAGCC AGCAGAGGGA GATCGCAGAG
    CTCCGACAGA ATCAGCTTGA TCTTCTGCAG AGAGTCACTA GCCACATGGA CGCAGTGCAG
    AGCTCCATCA TGGCTCACAT CGAGCATGCC ATGTTAGCTC AGCAAGAACA AGAACAGAGA
    AGAATGGAGC GAATTCTGGT CGAGGGTCAA AGTCGAAACC AACAGCTCCA GGACCAGCTA
    GTTCAGCAGC TCGTCCAAAC TCTGAACAAC AGTCTCTGCA ACCGGCTTGA GAAAGTTTTG
    CGTGAAGAAA TGAAGAAAAC CGTCCCGCAG ACCATATCGA AGAGTCTCGA ACCTGTGACC
    GGCCAGATGA ACAGCACCAT TGCTGCCAAG TTAACTGCAG TAGAAGGAGC ACTCAAAGAA
    AACGTCACCA AAGTTGTTAA GTCCAAGAAC ACTACAGATG CCATTGGTCG AGCTGCTGCA
    GAGGCCATGC AGGGTCCTAT TCAGGCAGCC TACAAAGAGA CCTTCCAGAG CATTGTCCTG
    CCTGTGTTTG AGAGAGGCTG TCAGTCCATG TTTCAGCAGA TCAATGACAG CTTCAAACAG
    GGAACCAACG AATACATCCA GCAGTTGGAG ACGCACATCA AAAACAGGAA ACAAAGAGAC
    CAGGATACAC GGGATCCTGT CATTGGCCAG TTACAGCAGA TGATTGACAG TCTTCAGAGT
    TCACAAGACC AGCTGGCGTC TACAGTGACG GCCAGCGTTA GCTCTGACGT CCAGCATCAG
    CTCCACATGA TTGTCGGAAA TCTGCAGGAC TCAATCCTGA CACAGGTGCA GCGTATAGTG
    AAGGGAGAGG TGAGTTTGGC TATGAAGGAG CAGCAGGCCG CCGTCACCTC CAGTATCATG
    CAGGCCATGC GCTCAGCCGC AGGAACACCC GTCCCATCCG CACATCTGGA CTACCAGGCC
    CAGCAGGCCA GCATATTACA GCTACTACAG CAGGGCCAGC TTAACGAGGC CTTCCAGCAG
    GCTTTATCCG CAGCAGACCT GAATCTGGTC CTGTACGTGT GCGAGACCAT CGACTCCCAA
    CAAGTGTTCG GCCAGCAGCC CTGTCCACTC CACCAGGCCG TCCTTCTGTC CCTCATCCAG
    CAGCTCTCCA CCAACCTGAG CACGCGCACA GAACTCAAGA TCAGCTATCT CGAAGATGCT
    GTGATGAACC TTGACCACGG AGATCCCGTC ACCCGAGACC ACATGTCCGC AGTGCTGACC
    CAGGTACGAC AGAAGCTCTT CCTGTTCCTG CAGCAGGACG CCCACAGCCC TATGAGCAAG
    CGTGCACGGC GCCTCATGAT GATGCTGCAG GGCCTGGTCA ACCACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa38041
    Clone ID Related Accession (Same CDS sequence) NM_001045085.1
    Accession Version NM_001045085.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4155bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-26
    Organism Danio rerio(zebrafish)
    Product enhancer of mRNA-decapping protein 4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX927240.11. On Sep 1, 2006 this sequence version replaced XM_688926.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCTTCCA GTTCCAGCAT GGACATAGAG GGTGCGACCC AGCACCTTCG GGACATCCTA 
    AAACTGGATC GTCCGGGAAA TTCGGCCGAA GCGTCTTTGG TCGAGAGCCA GAGAAAACCA
    TCTTTCAATG GAGAGCTGAA CGGAGTTCTC GGATCCGATC TCATGGGAGC AGCAATGGTG
    GAGCCGACGG TGCACAACTC AGACAAACTT AATGGACAGG ACACACAGAC CATCTGTCTT
    TCAGGAGATG ACGGGTCCAT TTGTGTCCCA ATCAGAGCGA ATATTGTGGA AATTGTGTCC
    AGCCGTGACT CTAGTATTGA CAGTAAGGCT CGTGGAAGCA ACAAGGTTAA AATTCAGCCA
    GTGGCCAAAT ATGACTGGGA GCACAAGTAC TACTATGGCA ATTTAATAGC CGTGTCCAAC
    ACGTACTTGG CTTATGCTAT CAGAGGTGCA AACAATCATT CAATGATTCG TGTGCTGCAC
    CTGAGCTCTA CAGAGCGCTC TCTGCTGAAG GGCTTCACCG GAGCTGTCAC AGACCTTGCC
    TTTGCCCATC TGGACTCCAC CCTGTTGGGA TGTGTGGACG AAGCCGGAAA CATGTTCATT
    TGGCAGCTTA CAAGTCACAG CAGCAAAATA CAAGACGAAG TGATTGTTCA CATCCGCAGA
    CCTGAGGACA CTCCACTAAA CTCTAACCGC AGACTCATCT GGTGTCCCTT CATCCCGGAG
    GACAATGACG AGAGCCCGGA GGATGCATGT CAGACATTAG CATTGCTTCA TGAAGACAGA
    GCTGAGGTTT GGGACCTCGA CATCCTCCGA TCAAATAACA GCTCATGGCC TGTGGACGCC
    ACTGAATTGA AAGAGGGTTT CATCACCATT AGAGGGCATG CAGCTCGCAT TAGTGAGGGA
    GCTCTGTCCC CTGATGGTAC AGTTTTGGCC ACAGCTAGTC ATGATGGATA TGTCAAATTT
    TGGCAAATTT ACATTGAAGG ACAGGACCAA CCCCGATGCC TGCATGAATG GCAGCCGCAT
    AATGGACAGC CTCTATCTTG CCTGCTGTTT TGTGACAACC ACAAGAAGCA AGATCCAGAC
    GTTCCATTCT GGCGCTTCCT GATTACTGGA GCTGATCAGA ACCAGGAGCT GAAGATGTGG
    TGCACAGTAT CATGGACCTG CCTACAAACC ATCAGGTTTT CTCCAGATCC ATTTAACTGC
    GGTGTGCTAC CCAGCCTGAA GGCCAGTTTG GACCTTTCTG CAGAATTTCT CATTCTTAGT
    GATGTTCAAA GGAAGGTATT GTATGTCATG GAGCTGCTGC AGGATCAAGA GAAAGGCCGT
    GCCAGTTTCA CTGCAGTCTC AGAGTTTCTG CTCACACACC CTGTGCTCAG CTTTGGTGTT
    CAGGATGTTT CTCGCGCTCG CCTGCGCCAT ACTGAGGTCC TTCCTCCTGA AGAAGAAAGC
    GAAAGCATGA CAGCAGAAGG AAACCAGGGA ACCTCTGAGT CCAGATCAGG AATTCAGATT
    AAACTCTACT GTGTGCATAC AAAATCACTA CAGGATGTGC AGATCTGGTT TCAGCCCAAT
    CAGGGGGACT CCTCTTCACT CTTCATGCCT CAGTCTGGAT CACAGGATGG GTTCAGTTTT
    TCTGACCCGC TGGCTGATCT GAATGTGGAG GCAATGAGCT CTGACAAGGA GTCTGGCGAT
    AGTGGTTCAC AAAATGACCT GAGTAAGATT CTGCCTCTTC CAAGTCCCGC TGACTTCATG
    AGTCCAGCCC CATCAGCCTT GCCCAAACTC ATGACCCCGG ATGCCTTCAT GACACCCAGC
    GCCTCTATGC CCGCTTCTCC TGGCAGCAGT GCTAGCAGTC TGACCATTGT CACGGCCATG
    AGCAGCTCAG ACAGCGGTGC AAGAGGTGGA GATGATCTGA CTCAGAGCCC TAAAATGTCT
    GTTGAATGTG TCAACAGCAG TTTCGCCAAT GCAGGCAGTC CAAGATCCAA CTCCATCCTC
    ATCTCAGGCC TTGGGGAAAA CATCCAGGTT TCCCCTCCAA ACCCTCCTCT CTCTCTGGAT
    CTCCAGGCAA TAGATCCCAT GGTTGTCCCA CAGGCGTCTC CAACAAGAGC TCGCTCACCG
    GATGTCATCT CATCTGCCTC TACTGCAATG TCCCAGGACA TACCTGAGAT TGCCTCCGAG
    ACGCTGCAGA GGGGACTGTC CGGTGCCAAT GCTGATTCGG GCCCCATCCT GCACTCTGAC
    AGCATGGCAT CAGCCGCATC AATTCTCCAC CTGCTATCTC CAAGGGCCCG GAGCAGCGCT
    GAGCACAGTT TGTTGCCTCT TGAATTGGGT GCAGCGTCTG TGGATGGAGA GCAGAGACTC
    AGTAACACAC CATCCCTGCT GGAGACTGCT CTCTCACAGG AAAACGCCGG AGCAGCCGGA
    GGATCCTGTT CTGACAGCAG TGTGAACCAC GCATGGCCTG CTGCTCCTGA TATCACGCGA
    GAGACGCGCA ACAGCCTGCG AGACAACGGT TTAGGAGACT GCTCTAGGGA GGAAATAAAA
    GACAGGCACA TTTCCTCCCC CTACCATAGA CGCACATATC ACCTGACTCA GAACGACAGT
    CAAGATGCAA GTGCAGAGCA GAGTGATCAT GACGATGAGG TTGCCAGTCT GGCCTCGTCC
    TCAGGGAACT GCGGCCCTCG CTCCTCTCAC AGACTTCCTG TAAAGGACTG GAAAACATCA
    CCCCGAAGCT CCCCTAAGCT CAAGCGGAAG AGCAAGAAAG ATGAAGGAGA GTCCTCGCAA
    TCTAGACAGA TTGAAAGCCA GATGAGCACA GAGGTGCAGG ACGAGCTCCT GCAGATGCTG
    CGGAGCCAGC AGAGGGAGAT CGCAGAGCTC CGACAGAATC AGCTTGATCT TCTGCAGAGA
    GTCACTAGCC ACATGGACGC AGTGCAGAGC TCCATGATGG CTCACATCGA GCATGCCATG
    TTAGCTCAGC AAGAACAAGA ACAGAGAAGA ATGGAGCGAA TTCTGGTCGA AGGTCAAAGT
    CGAAACCAAC AGCTCCAGGA CCAGCTAGTT CAGCAGCTCG TCCAAACTCT GAACAACAGT
    CTCTGCAACC GGCTTGAGAA AGTTTTGCGT GAAGAAATGA AGAAAACCGT CCCGCAGACC
    ATATCGAAGA GTCTCGAACC TGTGACCGGC CAGATGAACA GCACCATTGC TGCCAAGTTA
    ACTGCAGTAG AAGGAGCACT CAAAGAAAAC GTCACCAAAG TTGTTAAGTC CAAGAACACT
    ACAGATGCCA TTGGTCGAGC TGCTGCAGAG GCCATGCAGG GTCCTATTCA GGCAGCCTAC
    AAAGAGACCT TCCAGAGCAT TGTCCTGCCT GTGTTTGAGA GAGGCTGTCA GTCCATGTTT
    CAGCAGATCA ATGACAGCTT CAAACAGGGA ACCAACGAAT ACATCCAGCA GTTGGAGACG
    CACATCAAAA ACAGGAAACA AAGAGACCAG GATACACGGG ATCCTGTCAT TGGCCAGTTA
    CAGCAGATGA TTGACAGTCT TCAGAGTTCA CAAGACCAGC TGGCGTCTAC TGTGACGGCC
    AGCGTTAGCT CTGACGTCCA GCATCAGCTC CACATGATTG TCGGAAATCT GCAGGACTCA
    ATCCTGACGC AGGTGCAGCG TATAGTGAAG GGAGAGGTGA GTTTGGCTAT GAAGGAGCAG
    CAGGCCGCCG TCACCTCCAG TATCATGCAG GCCATGCGCT CGGCCGCAGG AACACCCGTC
    CCATCCGCAC ATCTGGACTA CCAGGCCCAG CAGGCCAGCA TATTACAGCT ACTACAGCAG
    GGCCAGCTTA ACGAGGCCTT CCAGCAGGCT TTATCCGCAG CAGACCTGAA TCTGGTCCTG
    TACGTGTGCG AGACCATCGA CTCCCAACAA GTGTTCGGCC AGCAGCCCTG TCCACTCCAC
    CAGGCCGTCC TTCTGTCCCT CATCCAGCAG CTCTCCACCA ACCTGAGCAC GCGCACAGAA
    CTCAAGATCA GCTATCTCGA AGATGCTGTG ATGAACCTTG ACCACGGAGA TCCCGTCACC
    CGAGACCACA TGTCCGCAGT GCTGACCCAG GTACGACAGA AGCTCTTCCT GTTCCTGCAG
    CAGGACGCCC ACAGCCCTAT GAGCAAGCGT GCACGGCGCC TCATGATGAT GCTGCAGGGC
    CTGGTCAACC ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001038550.1
    CDS1237..5391
    Translation

    Target ORF information:

    RefSeq Version NM_001045085.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio enhancer of mRNA decapping 4 (edc4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001045085.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGGCTTCCA GTTCCAGCAT GGACATAGAG GGTGCGACCC AGCACCTTCG GGACATCCTA 
    AAACTGGATC GTCCGGGAAA TTCGGCCGAA GCGTCTTTGG TCGAGAGCCA GAGAAAACCA
    TCTTTCAATG GAGAGCTGAA CGGAGTTCTC GGATCCGATC TCATGGGAGC AGCAATGGTG
    GAGCCGACGG TGCACAACTC AGACAAACTT AATGGACAGG ACACACAGAC CATCTGTCTT
    TCAGGAGATG ACGGGTCCAT TTGTGTCCCA ATCAGAGCGA ATATTGTGGA AATTGTGTCC
    AGCCGTGACT CTAGTATTGA CAGTAAGGCT CGTGGAAGCA ACAAGGTTAA AATTCAGCCA
    GTGGCCAAAT ATGACTGGGA GCACAAGTAC TACTATGGCA ATTTAATAGC CGTGTCCAAC
    ACGTACTTGG CTTATGCTAT CAGAGGTGCA AACAATCATT CAATGATTCG TGTGCTGCAC
    CTGAGCTCTA CAGAGCGCTC TCTGCTGAAG GGCTTCACCG GAGCTGTCAC AGACCTTGCC
    TTTGCCCATC TGGACTCCAC CCTGTTGGGA TGTGTGGACG AAGCCGGAAA CATGTTCATT
    TGGCAGCTTA CAAGTCACAG CAGCAAAATA CAAGACGAAG TGATTGTTCA CATCCGCAGA
    CCTGAGGACA CTCCACTAAA CTCTAACCGC AGACTCATCT GGTGTCCCTT CATCCCGGAG
    GACAATGACG AGAGCCCGGA GGATGCATGT CAGACATTAG CATTGCTTCA TGAAGACAGA
    GCTGAGGTTT GGGACCTCGA CATCCTCCGA TCAAATAACA GCTCATGGCC TGTGGACGCC
    ACTGAATTGA AAGAGGGTTT CATCACCATT AGAGGGCATG CAGCTCGCAT TAGTGAGGGA
    GCTCTGTCCC CTGATGGTAC AGTTTTGGCC ACAGCTAGTC ATGATGGATA TGTCAAATTT
    TGGCAAATTT ACATTGAAGG ACAGGACCAA CCCCGATGCC TGCATGAATG GCAGCCGCAT
    AATGGACAGC CTCTATCTTG CCTGCTGTTT TGTGACAACC ACAAGAAGCA AGATCCAGAC
    GTTCCATTCT GGCGCTTCCT GATTACTGGA GCTGATCAGA ACCAGGAGCT GAAGATGTGG
    TGCACAGTAT CATGGACCTG CCTACAAACC ATCAGGTTTT CTCCAGATCC ATTTAACTGC
    GGTGTGCTAC CCAGCCTGAA GGCCAGTTTG GACCTTTCTG CAGAATTTCT CATTCTTAGT
    GATGTTCAAA GGAAGGTATT GTATGTCATG GAGCTGCTGC AGGATCAAGA GAAAGGCCGT
    GCCAGTTTCA CTGCAGTCTC AGAGTTTCTG CTCACACACC CTGTGCTCAG CTTTGGTGTT
    CAGGATGTTT CTCGCGCTCG CCTGCGCCAT ACTGAGGTCC TTCCTCCTGA AGAAGAAAGC
    GAAAGCATGA CAGCAGAAGG AAACCAGGGA ACCTCTGAGT CCAGATCAGG AATTCAGATT
    AAACTCTACT GTGTGCATAC AAAATCACTA CAGGATGTGC AGATCTGGTT TCAGCCCAAT
    CAGGGGGACT CCTCTTCACT CTTCATGCCT CAGTCTGGAT CACAGGATGG GTTCAGTTTT
    TCTGACCCGC TGGCTGATCT GAATGTGGAG GCAATGAGCT CTGACAAGGA GTCTGGCGAT
    AGTGGTTCAC AAAATGACCT GAGTAAGATT CTGCCTCTTC CAAGTCCCGC TGACTTCATG
    AGTCCAGCCC CATCAGCCTT GCCCAAACTC ATGACCCCGG ATGCCTTCAT GACACCCAGC
    GCCTCTATGC CCGCTTCTCC TGGCAGCAGT GCTAGCAGTC TGACCATTGT CACGGCCATG
    AGCAGCTCAG ACAGCGGTGC AAGAGGTGGA GATGATCTGA CTCAGAGCCC TAAAATGTCT
    GTTGAATGTG TCAACAGCAG TTTCGCCAAT GCAGGCAGTC CAAGATCCAA CTCCATCCTC
    ATCTCAGGCC TTGGGGAAAA CATCCAGGTT TCCCCTCCAA ACCCTCCTCT CTCTCTGGAT
    CTCCAGGCAA TAGATCCCAT GGTTGTCCCA CAGGCGTCTC CAACAAGAGC TCGCTCACCG
    GATGTCATCT CATCTGCCTC TACTGCAATG TCCCAGGACA TACCTGAGAT TGCCTCCGAG
    ACGCTGCAGA GGGGACTGTC CGGTGCCAAT GCTGATTCGG GCCCCATCCT GCACTCTGAC
    AGCATGGCAT CAGCCGCATC AATTCTCCAC CTGCTATCTC CAAGGGCCCG GAGCAGCGCT
    GAGCACAGTT TGTTGCCTCT TGAATTGGGT GCAGCGTCTG TGGATGGAGA GCAGAGACTC
    AGTAACACAC CATCCCTGCT GGAGACTGCT CTCTCACAGG AAAACGCCGG AGCAGCCGGA
    GGATCCTGTT CTGACAGCAG TGTGAACCAC GCATGGCCTG CTGCTCCTGA TATCACGCGA
    GAGACGCGCA ACAGCCTGCG AGACAACGGT TTAGGAGACT GCTCTAGGGA GGAAATAAAA
    GACAGGCACA TTTCCTCCCC CTACCATAGA CGCACATATC ACCTGACTCA GAACGACAGT
    CAAGATGCAA GTGCAGAGCA GAGTGATCAT GACGATGAGG TTGCCAGTCT GGCCTCGTCC
    TCAGGGAACT GCGGCCCTCG CTCCTCTCAC AGACTTCCTG TAAAGGACTG GAAAACATCA
    CCCCGAAGCT CCCCTAAGCT CAAGCGGAAG AGCAAGAAAG ATGAAGGAGA GTCCTCGCAA
    TCTAGACAGA TTGAAAGCCA GATGAGCACA GAGGTGCAGG ACGAGCTCCT GCAGATGCTG
    CGGAGCCAGC AGAGGGAGAT CGCAGAGCTC CGACAGAATC AGCTTGATCT TCTGCAGAGA
    GTCACTAGCC ACATGGACGC AGTGCAGAGC TCCATGATGG CTCACATCGA GCATGCCATG
    TTAGCTCAGC AAGAACAAGA ACAGAGAAGA ATGGAGCGAA TTCTGGTCGA AGGTCAAAGT
    CGAAACCAAC AGCTCCAGGA CCAGCTAGTT CAGCAGCTCG TCCAAACTCT GAACAACAGT
    CTCTGCAACC GGCTTGAGAA AGTTTTGCGT GAAGAAATGA AGAAAACCGT CCCGCAGACC
    ATATCGAAGA GTCTCGAACC TGTGACCGGC CAGATGAACA GCACCATTGC TGCCAAGTTA
    ACTGCAGTAG AAGGAGCACT CAAAGAAAAC GTCACCAAAG TTGTTAAGTC CAAGAACACT
    ACAGATGCCA TTGGTCGAGC TGCTGCAGAG GCCATGCAGG GTCCTATTCA GGCAGCCTAC
    AAAGAGACCT TCCAGAGCAT TGTCCTGCCT GTGTTTGAGA GAGGCTGTCA GTCCATGTTT
    CAGCAGATCA ATGACAGCTT CAAACAGGGA ACCAACGAAT ACATCCAGCA GTTGGAGACG
    CACATCAAAA ACAGGAAACA AAGAGACCAG GATACACGGG ATCCTGTCAT TGGCCAGTTA
    CAGCAGATGA TTGACAGTCT TCAGAGTTCA CAAGACCAGC TGGCGTCTAC TGTGACGGCC
    AGCGTTAGCT CTGACGTCCA GCATCAGCTC CACATGATTG TCGGAAATCT GCAGGACTCA
    ATCCTGACGC AGGTGCAGCG TATAGTGAAG GGAGAGGTGA GTTTGGCTAT GAAGGAGCAG
    CAGGCCGCCG TCACCTCCAG TATCATGCAG GCCATGCGCT CGGCCGCAGG AACACCCGTC
    CCATCCGCAC ATCTGGACTA CCAGGCCCAG CAGGCCAGCA TATTACAGCT ACTACAGCAG
    GGCCAGCTTA ACGAGGCCTT CCAGCAGGCT TTATCCGCAG CAGACCTGAA TCTGGTCCTG
    TACGTGTGCG AGACCATCGA CTCCCAACAA GTGTTCGGCC AGCAGCCCTG TCCACTCCAC
    CAGGCCGTCC TTCTGTCCCT CATCCAGCAG CTCTCCACCA ACCTGAGCAC GCGCACAGAA
    CTCAAGATCA GCTATCTCGA AGATGCTGTG ATGAACCTTG ACCACGGAGA TCCCGTCACC
    CGAGACCACA TGTCCGCAGT GCTGACCCAG GTACGACAGA AGCTCTTCCT GTTCCTGCAG
    CAGGACGCCC ACAGCCCTAT GAGCAAGCGT GCACGGCGCC TCATGATGAT GCTGCAGGGC
    CTGGTCAACC ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44697
    Clone ID Related Accession (Same CDS sequence) XM_009293574.2
    Accession Version XM_009293574.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4275bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product enhancer of mRNA-decapping protein 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293574.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTTCCA GTTCCAGCAT GGACATAGAG GGTGCGACCC AGCACCTTCG GGACATCCTA 
    AAACTGGATC GTCCGGGAAA TTCGGCCGAA GCGTCTTTGG TCGAGAGCCA GAGAAAACCA
    TCTTTCAATG GAGAGCTGAA CGGAGTTCTC GGATCCGATC TCATGGGAGC AGCAATGGTG
    GAGCCGACGG TGCACAACTC AGACAAACTT AATGGACAGG ACACACAGAC CATCTGTCTT
    TCAGGAGATG ACGGGTCCAT TTGTGTCCCA ATCAGAGCGA ATATTGTGGA AATTGTGTCC
    AGCCGTGACT CTAGTATTGA CAGTAAGGCT CGTGGAAGCA ACAAGGTTAA AATTCAGCCA
    GTGGCCAAAT ATGACTGGGA GCACAAGTAC TACTATGGCA ATTTAATAGC CGTGTCCAAC
    ACGTACTTGG CTTATGCTAT CAGAGGTGCA AACAATCATT CAATGATTCG TGTGCTGCAC
    CTGAGCTCTA CAGAGCGCTC TCTGCTGAAG GGCTTCACCG GGGCTGTCAC AGACCTTGCC
    TTTGCCCATC TGGACTCCAC CCTGTTGGGA TGTGTGGACG AAGCCGGAAA CATGTTCATT
    TGGCAGCTTA CAAGCCACAG CAGCAAAATA CAAGACGAAG TGATTGTTCA CATCCGCAGA
    CCTGAGGACA CTCCACTAAA CTCTAACCGC AGACTCATCT GGTGTCCCTT CATCCCGGAG
    GACAATGACG AGAGCCCAGA GGATGCATGT CAGACATTAG CATTGCTTCA TGAAGACAGA
    GCTGAGGTTT GGGACCTCGA CATCCTCCGA TCAAATAACA GCTCATGGCC TGTGGACGCC
    ACTGAATTGA AAGAGGGTTT CATCACCATT AGAGGGCATG CAGCTCGCAT AAGTGAGGGA
    GCTCTGTCCC CTGATGGTAC GGTTTTGGCC ACAGCTAGTC ATGATGGATA TGTCAAATTT
    TGGCAAATTT ACATTGAAGG ACAGGACCAA CCCCGATGCC TGCATGAATG GCAGCCGCAT
    AATGGACAGC CTCTATCTTG CCTGCTGTTT TGTGACAACC ACAAGAAGCA AGATCCAGAC
    GTTCCATTCT GGCGCTTCCT GATTACTGGA GCTGATCAGA ACCAGGAGCT GAAGATGTGG
    TGCACAGTAT CATGGACCTG CCTACAAACC ATCAGGTTTT CTCCAGATCC ATTTAACTGC
    GGTGTGCTAC CCAGCCTGAA GGCCAGTTTG GACCTTTCTG CAGAATTTCT CATTCTTAGT
    GATGTTCAAA GGAAGGTATT GTATGTCATG GAGCTGCTGC AGGATCAAGA GAAAGGCCGT
    GCCAGTTTCA CTGCAGTCTC AGAGTTTCTG CTCACACACC CTGTGCTCAG CTTCGGTGTT
    CAGGATGTTT CTCGTGCTCG CCTGCGCCAT ACTGAGGTCC TTCCTCCTGA AGAAGAAAGC
    GAAAGCATGA CAGCAGAAGG AAACCAGGGA ACCTCTGAGT CCAGATCAGG AATTCAGATT
    AAACTCTACT GTGTGCATAC AAAATCACTA CAGGATGTGC AGATCTGGTT TCAGCCCAAT
    CAGGGGGACT CCTCTTCACT CTTCATGCCT CAGTCTGGAT CACAGGATGG GTTCAGTTTT
    TCTGACCCGC TGGCTGATCT GAATGTGGAG GCAATGAGCT CTGACAAGGA GTCTGGCGAT
    AGTGGTTCAC AAAATGACCT GAGTAAGATT CTGCCTCTTC CAAGTCCCGC TGACTTCATG
    AGTCCAGCCC CATCAGCCTT GCCCAAACTC ATGACCCCGG ATGCCTTCAT GACACCCAGC
    GCCTCTATGC CCGCTTCTCC TGGCAGCAGT GCTAGCAGTC TGACCATTGT CACGGCCATG
    AGCAGCTCAG ACAGCGGTGC AAGAGGTGGA GATGATCTGA CTCAGAGCCC TAAAATGTCT
    GTTGAATGTG TCAACAGCAG TTTCGCCAAT GCAGGCAGTC CAAGATCCAA CTCCATCCTC
    ATCTCAGGCC TTGGGGAAAA CATCCAGGCT TTCGCTCCCC CACTGGTTCT TTCTTCATCC
    CTCCTTCAAG AAGCCTCTCT GCTCTGCGAA GCTGCTCCTT ATTCTCACCG ACTCCCCCTC
    CCTTATTTCC CATCTCCTCC TCCGCAGGTT TCCCCTCCAA ACCCTCCTCT CTCTCTGGAT
    CTCCAGGCAA TAGATCCCAT GGTTGTCCCA CAGGCGTCTC CAACAAGAGC TCGCTCACCG
    GATGTCATCT CATCTGCCTC TACTGCAATG TCCCAGGACA TACCTGAGAT TGCCTCCGAG
    ACGCTGCAGA GGGGACTGTC CGGTGCCAAT GCTGATTCGG GCCCCATCCT GCACTCTGAC
    AGCATGGCAT CAGCCGCATC AATTCTCCAC CTGCTATCTC CAAGGGCCCG GAGCAGCGCT
    GAGCACAGTT TGTTGCCTCT TGAATTGGGT GCAGCGTCTG TGGATGGAGA GCAGAGACTC
    AGTAACACAC CATCCCTGCT GGAGACTGCT CTCTCACAGG AAAACGCCGG AGCAGCCGGA
    GGATCCTGTT CTGACAGCAG TGTGAACCAC GCATGGCCTG CTGCTCCTGA TATCACGCGA
    GAGACGCGCA ACAGCCTGCG AGACAACGGT TTAGGAGACT GCTCTAGGGA GGAAATAAAA
    GACAGGCACA TTTCCTCCCC CTACCATAGA CGCACATATC ACCTGACTCA GAACGACAGT
    CAAGATGCAA GTGCAGAGCA GAGTGATCAT GACGATGAGG TTGCCAGTCT GGCCTCGTCC
    TCAGGGAACT GCGGCCCTCG CTCCTCTCAC AGACTTCCTG TAAAGGACTG GAAAACATCA
    CCCCGCAGCT CCCCTAAGCT CAAGCGGAAG AGCAAGAAAG ATGAAGGAGA GTCCTCGCAA
    TCTAGACAGA TTGAAAGCCA GATGAGCACA GAGGTGCAGG ACGAGCTCCT GCAGATGCTG
    CGGAGCCAGC AGAGGGAGAT CGCAGAGCTC CGACAGAATC AGCTTGATCT TCTGCAGAGA
    GTCACTAGCC ACATGGACGC AGTGCAGAGC TCCATCATGG CTCACATCGA GCATGCCATG
    TTAGCTCAGC AAGAACAAGA ACAGAGAAGA ATGGAGCGAA TTCTGGTCGA GGGTCAAAGT
    CGAAACCAAC AGCTCCAGGA CCAGCTAGTT CAGCAGCTCG TCCAAACTCT GAACAACAGT
    CTCTGCAACC GGCTTGAGAA AGTTTTGCGT GAAGAAATGA AGAAAACCGT CCCGCAGACC
    ATATCGAAGA GTCTCGAACC TGTGACCGGC CAGATGAACA GCACCATTGC TGCCAAGTTA
    ACTGCAGTAG AAGGAGCACT CAAAGAAAAC GTCACCAAAG TTGTTAAGTC CAAGAACACT
    ACAGATGCCA TTGGTCGAGC TGCTGCAGAG GCCATGCAGG GTCCTATTCA GGCAGCCTAC
    AAAGAGACCT TCCAGAGCAT TGTCCTGCCT GTGTTTGAGA GAGGCTGTCA GTCCATGTTT
    CAGCAGATCA ATGACAGCTT CAAACAGGGA ACCAACGAAT ACATCCAGCA GTTGGAGACG
    CACATCAAAA ACAGGAAACA AAGAGACCAG GATACACGGG ATCCTGTCAT TGGCCAGTTA
    CAGCAGATGA TTGACAGTCT TCAGAGTTCA CAAGACCAGC TGGCGTCTAC AGTGACGGCC
    AGCGTTAGCT CTGACGTCCA GCATCAGCTC CACATGATTG TCGGAAATCT GCAGGACTCA
    ATCCTGACAC AGGTGCAGCG TATAGTGAAG GGAGAGGTGA GTTTGGCTAT GAAGGAGCAG
    CAGGCCGCCG TCACCTCCAG TATCATGCAG GCCATGCGCT CAGCCGCAGG AACACCCGTC
    CCATCCGCAC ATCTGGACTA CCAGGCCCAG CAGGCCAGCA TATTACAGCT ACTACAGCAG
    GGCCAGCTTA ACGAGGCCTT CCAGCAGGCT TTATCCGCAG CAGACCTGAA TCTGGTCCTG
    TACGTGTGCG AGACCATCGA CTCCCAACAA GTGTTCGGCC AGCAGCCCTG TCCACTCCAC
    CAGGCCGTCC TTCTGTCCCT CATCCAGCAG CTCTCCACCA ACCTGAGCAC GCGCACAGAA
    CTCAAGATCA GCTATCTCGA AGATGCTGTG ATGAACCTTG ACCACGGAGA TCCCGTCACC
    CGAGACCACA TGTCCGCAGT GCTGACCCAG GTACGACAGA AGCTCTTCCT GTTCCTGCAG
    CAGGACGCCC ACAGCCCTAT GAGCAAGCGT GCACGGCGCC TCATGATGAT GCTGCAGGGC
    CTGGTCAACC ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291849.1
    CDS132..4406
    Translation

    Target ORF information:

    RefSeq Version XM_009293574.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio enhancer of mRNA decapping 4 (edc4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293574.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGCTTCCA GTTCCAGCAT GGACATAGAG GGTGCGACCC AGCACCTTCG GGACATCCTA 
    AAACTGGATC GTCCGGGAAA TTCGGCCGAA GCGTCTTTGG TCGAGAGCCA GAGAAAACCA
    TCTTTCAATG GAGAGCTGAA CGGAGTTCTC GGATCCGATC TCATGGGAGC AGCAATGGTG
    GAGCCGACGG TGCACAACTC AGACAAACTT AATGGACAGG ACACACAGAC CATCTGTCTT
    TCAGGAGATG ACGGGTCCAT TTGTGTCCCA ATCAGAGCGA ATATTGTGGA AATTGTGTCC
    AGCCGTGACT CTAGTATTGA CAGTAAGGCT CGTGGAAGCA ACAAGGTTAA AATTCAGCCA
    GTGGCCAAAT ATGACTGGGA GCACAAGTAC TACTATGGCA ATTTAATAGC CGTGTCCAAC
    ACGTACTTGG CTTATGCTAT CAGAGGTGCA AACAATCATT CAATGATTCG TGTGCTGCAC
    CTGAGCTCTA CAGAGCGCTC TCTGCTGAAG GGCTTCACCG GGGCTGTCAC AGACCTTGCC
    TTTGCCCATC TGGACTCCAC CCTGTTGGGA TGTGTGGACG AAGCCGGAAA CATGTTCATT
    TGGCAGCTTA CAAGCCACAG CAGCAAAATA CAAGACGAAG TGATTGTTCA CATCCGCAGA
    CCTGAGGACA CTCCACTAAA CTCTAACCGC AGACTCATCT GGTGTCCCTT CATCCCGGAG
    GACAATGACG AGAGCCCAGA GGATGCATGT CAGACATTAG CATTGCTTCA TGAAGACAGA
    GCTGAGGTTT GGGACCTCGA CATCCTCCGA TCAAATAACA GCTCATGGCC TGTGGACGCC
    ACTGAATTGA AAGAGGGTTT CATCACCATT AGAGGGCATG CAGCTCGCAT AAGTGAGGGA
    GCTCTGTCCC CTGATGGTAC GGTTTTGGCC ACAGCTAGTC ATGATGGATA TGTCAAATTT
    TGGCAAATTT ACATTGAAGG ACAGGACCAA CCCCGATGCC TGCATGAATG GCAGCCGCAT
    AATGGACAGC CTCTATCTTG CCTGCTGTTT TGTGACAACC ACAAGAAGCA AGATCCAGAC
    GTTCCATTCT GGCGCTTCCT GATTACTGGA GCTGATCAGA ACCAGGAGCT GAAGATGTGG
    TGCACAGTAT CATGGACCTG CCTACAAACC ATCAGGTTTT CTCCAGATCC ATTTAACTGC
    GGTGTGCTAC CCAGCCTGAA GGCCAGTTTG GACCTTTCTG CAGAATTTCT CATTCTTAGT
    GATGTTCAAA GGAAGGTATT GTATGTCATG GAGCTGCTGC AGGATCAAGA GAAAGGCCGT
    GCCAGTTTCA CTGCAGTCTC AGAGTTTCTG CTCACACACC CTGTGCTCAG CTTCGGTGTT
    CAGGATGTTT CTCGTGCTCG CCTGCGCCAT ACTGAGGTCC TTCCTCCTGA AGAAGAAAGC
    GAAAGCATGA CAGCAGAAGG AAACCAGGGA ACCTCTGAGT CCAGATCAGG AATTCAGATT
    AAACTCTACT GTGTGCATAC AAAATCACTA CAGGATGTGC AGATCTGGTT TCAGCCCAAT
    CAGGGGGACT CCTCTTCACT CTTCATGCCT CAGTCTGGAT CACAGGATGG GTTCAGTTTT
    TCTGACCCGC TGGCTGATCT GAATGTGGAG GCAATGAGCT CTGACAAGGA GTCTGGCGAT
    AGTGGTTCAC AAAATGACCT GAGTAAGATT CTGCCTCTTC CAAGTCCCGC TGACTTCATG
    AGTCCAGCCC CATCAGCCTT GCCCAAACTC ATGACCCCGG ATGCCTTCAT GACACCCAGC
    GCCTCTATGC CCGCTTCTCC TGGCAGCAGT GCTAGCAGTC TGACCATTGT CACGGCCATG
    AGCAGCTCAG ACAGCGGTGC AAGAGGTGGA GATGATCTGA CTCAGAGCCC TAAAATGTCT
    GTTGAATGTG TCAACAGCAG TTTCGCCAAT GCAGGCAGTC CAAGATCCAA CTCCATCCTC
    ATCTCAGGCC TTGGGGAAAA CATCCAGGCT TTCGCTCCCC CACTGGTTCT TTCTTCATCC
    CTCCTTCAAG AAGCCTCTCT GCTCTGCGAA GCTGCTCCTT ATTCTCACCG ACTCCCCCTC
    CCTTATTTCC CATCTCCTCC TCCGCAGGTT TCCCCTCCAA ACCCTCCTCT CTCTCTGGAT
    CTCCAGGCAA TAGATCCCAT GGTTGTCCCA CAGGCGTCTC CAACAAGAGC TCGCTCACCG
    GATGTCATCT CATCTGCCTC TACTGCAATG TCCCAGGACA TACCTGAGAT TGCCTCCGAG
    ACGCTGCAGA GGGGACTGTC CGGTGCCAAT GCTGATTCGG GCCCCATCCT GCACTCTGAC
    AGCATGGCAT CAGCCGCATC AATTCTCCAC CTGCTATCTC CAAGGGCCCG GAGCAGCGCT
    GAGCACAGTT TGTTGCCTCT TGAATTGGGT GCAGCGTCTG TGGATGGAGA GCAGAGACTC
    AGTAACACAC CATCCCTGCT GGAGACTGCT CTCTCACAGG AAAACGCCGG AGCAGCCGGA
    GGATCCTGTT CTGACAGCAG TGTGAACCAC GCATGGCCTG CTGCTCCTGA TATCACGCGA
    GAGACGCGCA ACAGCCTGCG AGACAACGGT TTAGGAGACT GCTCTAGGGA GGAAATAAAA
    GACAGGCACA TTTCCTCCCC CTACCATAGA CGCACATATC ACCTGACTCA GAACGACAGT
    CAAGATGCAA GTGCAGAGCA GAGTGATCAT GACGATGAGG TTGCCAGTCT GGCCTCGTCC
    TCAGGGAACT GCGGCCCTCG CTCCTCTCAC AGACTTCCTG TAAAGGACTG GAAAACATCA
    CCCCGCAGCT CCCCTAAGCT CAAGCGGAAG AGCAAGAAAG ATGAAGGAGA GTCCTCGCAA
    TCTAGACAGA TTGAAAGCCA GATGAGCACA GAGGTGCAGG ACGAGCTCCT GCAGATGCTG
    CGGAGCCAGC AGAGGGAGAT CGCAGAGCTC CGACAGAATC AGCTTGATCT TCTGCAGAGA
    GTCACTAGCC ACATGGACGC AGTGCAGAGC TCCATCATGG CTCACATCGA GCATGCCATG
    TTAGCTCAGC AAGAACAAGA ACAGAGAAGA ATGGAGCGAA TTCTGGTCGA GGGTCAAAGT
    CGAAACCAAC AGCTCCAGGA CCAGCTAGTT CAGCAGCTCG TCCAAACTCT GAACAACAGT
    CTCTGCAACC GGCTTGAGAA AGTTTTGCGT GAAGAAATGA AGAAAACCGT CCCGCAGACC
    ATATCGAAGA GTCTCGAACC TGTGACCGGC CAGATGAACA GCACCATTGC TGCCAAGTTA
    ACTGCAGTAG AAGGAGCACT CAAAGAAAAC GTCACCAAAG TTGTTAAGTC CAAGAACACT
    ACAGATGCCA TTGGTCGAGC TGCTGCAGAG GCCATGCAGG GTCCTATTCA GGCAGCCTAC
    AAAGAGACCT TCCAGAGCAT TGTCCTGCCT GTGTTTGAGA GAGGCTGTCA GTCCATGTTT
    CAGCAGATCA ATGACAGCTT CAAACAGGGA ACCAACGAAT ACATCCAGCA GTTGGAGACG
    CACATCAAAA ACAGGAAACA AAGAGACCAG GATACACGGG ATCCTGTCAT TGGCCAGTTA
    CAGCAGATGA TTGACAGTCT TCAGAGTTCA CAAGACCAGC TGGCGTCTAC AGTGACGGCC
    AGCGTTAGCT CTGACGTCCA GCATCAGCTC CACATGATTG TCGGAAATCT GCAGGACTCA
    ATCCTGACAC AGGTGCAGCG TATAGTGAAG GGAGAGGTGA GTTTGGCTAT GAAGGAGCAG
    CAGGCCGCCG TCACCTCCAG TATCATGCAG GCCATGCGCT CAGCCGCAGG AACACCCGTC
    CCATCCGCAC ATCTGGACTA CCAGGCCCAG CAGGCCAGCA TATTACAGCT ACTACAGCAG
    GGCCAGCTTA ACGAGGCCTT CCAGCAGGCT TTATCCGCAG CAGACCTGAA TCTGGTCCTG
    TACGTGTGCG AGACCATCGA CTCCCAACAA GTGTTCGGCC AGCAGCCCTG TCCACTCCAC
    CAGGCCGTCC TTCTGTCCCT CATCCAGCAG CTCTCCACCA ACCTGAGCAC GCGCACAGAA
    CTCAAGATCA GCTATCTCGA AGATGCTGTG ATGAACCTTG ACCACGGAGA TCCCGTCACC
    CGAGACCACA TGTCCGCAGT GCTGACCCAG GTACGACAGA AGCTCTTCCT GTTCCTGCAG
    CAGGACGCCC ACAGCCCTAT GAGCAAGCGT GCACGGCGCC TCATGATGAT GCTGCAGGGC
    CTGGTCAACC ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44698
    Clone ID Related Accession (Same CDS sequence) XM_009293575.2
    Accession Version XM_009293575.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4221bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product enhancer of mRNA-decapping protein 4 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293575.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTTCCA GTTCCAGCAT GGACATAGAG GGTGCGACCC AGCACCTTCG GGACATCCTA 
    AAACTGGATC GTCCGGGAAA TTCGGCCGAA GCGTCTTTGG TCGAGAGCCA GAGAAAACCA
    TCTTTCAATG GAGAGCTGAA CGGAGTTCTC GGATCCGATC TCATGGGAGC AGCAATGGTG
    GAGCCGACGG TGCACAACTC AGACAAACTT AATGGACAGG ACACACAGAC CATCTGTCTT
    TCAGGAGATG ACGGGTCCAT TTGTGTCCCA ATCAGAGCGA ATATTGTGGA AATTGTGTCC
    AGCCGTGACT CTAGTATTGA CAGTAAGGCT CGTGGAAGCA ACAAGGTTAA AATTCAGCCA
    GTGGCCAAAT ATGACTGGGA GCACAAGTAC TACTATGGCA ATTTAATAGC CGTGTCCAAC
    ACGTACTTGG CTTATGCTAT CAGAGGTGCA AACAATCATT CAATGATTCG TGTGCTGCAC
    CTGAGCTCTA CAGAGCGCTC TCTGCTGAAG GGCTTCACCG GGGCTGTCAC AGACCTTGCC
    TTTGCCCATC TGGACTCCAC CCTGTTGGGA TGTGTGGACG AAGCCGGAAA CATGTTCATT
    TGGCAGCTTA CAAGCCACAG CAGCAAAATA CAAGACGAAG TGATTGTTCA CATCCGCAGA
    CCTGAGGACA CTCCACTAAA CTCTAACCGC AGACTCATCT GGTGTCCCTT CATCCCGGAG
    GACAATGACG AGAGCCCAGA GGATGCATGT CAGACATTAG CATTGCTTCA TGAAGACAGA
    GCTGAGGTTT GGGACCTCGA CATCCTCCGA TCAAATAACA GCTCATGGCC TGTGGACGCC
    ACTGAATTGA AAGAGGGTTT CATCACCATT AGAGGGCATG CAGCTCGCAT AAGTGAGGGA
    GCTCTGTCCC CTGATGGTAC GGTTTTGGCC ACAGCTAGTC ATGATGGATA TGTCAAATTT
    TGGCAAATTT ACATTGAAGG ACAGGACCAA CCCCGATGCC TGCATGAATG GCAGCCGCAT
    AATGGACAGC CTCTATCTTG CCTGCTGTTT TGTGACAACC ACAAGAAGCA AGATCCAGAC
    GTTCCATTCT GGCGCTTCCT GATTACTGGA GCTGATCAGA ACCAGGAGCT GAAGATGTGG
    TGCACAGTAT CATGGACCTG CCTACAAACC ATCAGGTTTT CTCCAGATCC ATTTAACTGC
    GGTGTGCTAC CCAGCCTGAA GGCCAGTTTG GACCTTTCTG CAGAATTTCT CATTCTTAGT
    GATGTTCAAA GGAAGGTATT GTATGTCATG GAGCTGCTGC AGGATCAAGA GAAAGGCCGT
    GCCAGTTTCA CTGCAGTCTC AGAGTTTCTG CTCACACACC CTGTGCTCAG CTTCGGTGTT
    CAGGATGTTT CTCGTGCTCG CCTGCGCCAT ACTGAGGTCC TTCCTCCTGA AGAAGAAAGC
    GAAAGCATGA CAGCAGAAGG AAACCAGGGA ACCTCTGAGT CCAGATCAGG AATTCAGATT
    AAACTCTACT GTGTGCATAC AAAATCACTA CAGGATGTGC AGATCTGGTT TCAGCCCAAT
    CAGGGGGACT CCTCTTCACT CTTCATGCCT CAGTCTGGAT CACAGGATGG GTTCAGTTTT
    TCTGACCCGC TGGCTGATCT GAATGTGGAG GCAATGAGCT CTGACAAGGA GTCTGGCGAT
    AGTGGTTCAC AAAATGACCT GAGTAAGATT CTGCCTCTTC CAAGTCCCGC TGACTTCATG
    AGTCCAGCCC CATCAGCCTT GCCCAAACTC ATGACCCCGG ATGCCTTCAT GACACCCAGC
    GCCTCTATGC CCGCTTCTCC TGGCAGCAGT GCTAGCAGTC TGACCATTGT CACGGCCATG
    AGCAGCTCAG ACAGCGGTGC AAGCAGTTTC GCCAATGCAG GCAGTCCAAG ATCCAACTCC
    ATCCTCATCT CAGGCCTTGG GGAAAACATC CAGGCTTTCG CTCCCCCACT GGTTCTTTCT
    TCATCCCTCC TTCAAGAAGC CTCTCTGCTC TGCGAAGCTG CTCCTTATTC TCACCGACTC
    CCCCTCCCTT ATTTCCCATC TCCTCCTCCG CAGGTTTCCC CTCCAAACCC TCCTCTCTCT
    CTGGATCTCC AGGCAATAGA TCCCATGGTT GTCCCACAGG CGTCTCCAAC AAGAGCTCGC
    TCACCGGATG TCATCTCATC TGCCTCTACT GCAATGTCCC AGGACATACC TGAGATTGCC
    TCCGAGACGC TGCAGAGGGG ACTGTCCGGT GCCAATGCTG ATTCGGGCCC CATCCTGCAC
    TCTGACAGCA TGGCATCAGC CGCATCAATT CTCCACCTGC TATCTCCAAG GGCCCGGAGC
    AGCGCTGAGC ACAGTTTGTT GCCTCTTGAA TTGGGTGCAG CGTCTGTGGA TGGAGAGCAG
    AGACTCAGTA ACACACCATC CCTGCTGGAG ACTGCTCTCT CACAGGAAAA CGCCGGAGCA
    GCCGGAGGAT CCTGTTCTGA CAGCAGTGTG AACCACGCAT GGCCTGCTGC TCCTGATATC
    ACGCGAGAGA CGCGCAACAG CCTGCGAGAC AACGGTTTAG GAGACTGCTC TAGGGAGGAA
    ATAAAAGACA GGCACATTTC CTCCCCCTAC CATAGACGCA CATATCACCT GACTCAGAAC
    GACAGTCAAG ATGCAAGTGC AGAGCAGAGT GATCATGACG ATGAGGTTGC CAGTCTGGCC
    TCGTCCTCAG GGAACTGCGG CCCTCGCTCC TCTCACAGAC TTCCTGTAAA GGACTGGAAA
    ACATCACCCC GCAGCTCCCC TAAGCTCAAG CGGAAGAGCA AGAAAGATGA AGGAGAGTCC
    TCGCAATCTA GACAGATTGA AAGCCAGATG AGCACAGAGG TGCAGGACGA GCTCCTGCAG
    ATGCTGCGGA GCCAGCAGAG GGAGATCGCA GAGCTCCGAC AGAATCAGCT TGATCTTCTG
    CAGAGAGTCA CTAGCCACAT GGACGCAGTG CAGAGCTCCA TCATGGCTCA CATCGAGCAT
    GCCATGTTAG CTCAGCAAGA ACAAGAACAG AGAAGAATGG AGCGAATTCT GGTCGAGGGT
    CAAAGTCGAA ACCAACAGCT CCAGGACCAG CTAGTTCAGC AGCTCGTCCA AACTCTGAAC
    AACAGTCTCT GCAACCGGCT TGAGAAAGTT TTGCGTGAAG AAATGAAGAA AACCGTCCCG
    CAGACCATAT CGAAGAGTCT CGAACCTGTG ACCGGCCAGA TGAACAGCAC CATTGCTGCC
    AAGTTAACTG CAGTAGAAGG AGCACTCAAA GAAAACGTCA CCAAAGTTGT TAAGTCCAAG
    AACACTACAG ATGCCATTGG TCGAGCTGCT GCAGAGGCCA TGCAGGGTCC TATTCAGGCA
    GCCTACAAAG AGACCTTCCA GAGCATTGTC CTGCCTGTGT TTGAGAGAGG CTGTCAGTCC
    ATGTTTCAGC AGATCAATGA CAGCTTCAAA CAGGGAACCA ACGAATACAT CCAGCAGTTG
    GAGACGCACA TCAAAAACAG GAAACAAAGA GACCAGGATA CACGGGATCC TGTCATTGGC
    CAGTTACAGC AGATGATTGA CAGTCTTCAG AGTTCACAAG ACCAGCTGGC GTCTACAGTG
    ACGGCCAGCG TTAGCTCTGA CGTCCAGCAT CAGCTCCACA TGATTGTCGG AAATCTGCAG
    GACTCAATCC TGACACAGGT GCAGCGTATA GTGAAGGGAG AGGTGAGTTT GGCTATGAAG
    GAGCAGCAGG CCGCCGTCAC CTCCAGTATC ATGCAGGCCA TGCGCTCAGC CGCAGGAACA
    CCCGTCCCAT CCGCACATCT GGACTACCAG GCCCAGCAGG CCAGCATATT ACAGCTACTA
    CAGCAGGGCC AGCTTAACGA GGCCTTCCAG CAGGCTTTAT CCGCAGCAGA CCTGAATCTG
    GTCCTGTACG TGTGCGAGAC CATCGACTCC CAACAAGTGT TCGGCCAGCA GCCCTGTCCA
    CTCCACCAGG CCGTCCTTCT GTCCCTCATC CAGCAGCTCT CCACCAACCT GAGCACGCGC
    ACAGAACTCA AGATCAGCTA TCTCGAAGAT GCTGTGATGA ACCTTGACCA CGGAGATCCC
    GTCACCCGAG ACCACATGTC CGCAGTGCTG ACCCAGGTAC GACAGAAGCT CTTCCTGTTC
    CTGCAGCAGG ACGCCCACAG CCCTATGAGC AAGCGTGCAC GGCGCCTCAT GATGATGCTG
    CAGGGCCTGG TCAACCACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291850.1
    CDS132..4352
    Translation

    Target ORF information:

    RefSeq Version XM_009293575.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio enhancer of mRNA decapping 4 (edc4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293575.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    ATGGCTTCCA GTTCCAGCAT GGACATAGAG GGTGCGACCC AGCACCTTCG GGACATCCTA 
    AAACTGGATC GTCCGGGAAA TTCGGCCGAA GCGTCTTTGG TCGAGAGCCA GAGAAAACCA
    TCTTTCAATG GAGAGCTGAA CGGAGTTCTC GGATCCGATC TCATGGGAGC AGCAATGGTG
    GAGCCGACGG TGCACAACTC AGACAAACTT AATGGACAGG ACACACAGAC CATCTGTCTT
    TCAGGAGATG ACGGGTCCAT TTGTGTCCCA ATCAGAGCGA ATATTGTGGA AATTGTGTCC
    AGCCGTGACT CTAGTATTGA CAGTAAGGCT CGTGGAAGCA ACAAGGTTAA AATTCAGCCA
    GTGGCCAAAT ATGACTGGGA GCACAAGTAC TACTATGGCA ATTTAATAGC CGTGTCCAAC
    ACGTACTTGG CTTATGCTAT CAGAGGTGCA AACAATCATT CAATGATTCG TGTGCTGCAC
    CTGAGCTCTA CAGAGCGCTC TCTGCTGAAG GGCTTCACCG GGGCTGTCAC AGACCTTGCC
    TTTGCCCATC TGGACTCCAC CCTGTTGGGA TGTGTGGACG AAGCCGGAAA CATGTTCATT
    TGGCAGCTTA CAAGCCACAG CAGCAAAATA CAAGACGAAG TGATTGTTCA CATCCGCAGA
    CCTGAGGACA CTCCACTAAA CTCTAACCGC AGACTCATCT GGTGTCCCTT CATCCCGGAG
    GACAATGACG AGAGCCCAGA GGATGCATGT CAGACATTAG CATTGCTTCA TGAAGACAGA
    GCTGAGGTTT GGGACCTCGA CATCCTCCGA TCAAATAACA GCTCATGGCC TGTGGACGCC
    ACTGAATTGA AAGAGGGTTT CATCACCATT AGAGGGCATG CAGCTCGCAT AAGTGAGGGA
    GCTCTGTCCC CTGATGGTAC GGTTTTGGCC ACAGCTAGTC ATGATGGATA TGTCAAATTT
    TGGCAAATTT ACATTGAAGG ACAGGACCAA CCCCGATGCC TGCATGAATG GCAGCCGCAT
    AATGGACAGC CTCTATCTTG CCTGCTGTTT TGTGACAACC ACAAGAAGCA AGATCCAGAC
    GTTCCATTCT GGCGCTTCCT GATTACTGGA GCTGATCAGA ACCAGGAGCT GAAGATGTGG
    TGCACAGTAT CATGGACCTG CCTACAAACC ATCAGGTTTT CTCCAGATCC ATTTAACTGC
    GGTGTGCTAC CCAGCCTGAA GGCCAGTTTG GACCTTTCTG CAGAATTTCT CATTCTTAGT
    GATGTTCAAA GGAAGGTATT GTATGTCATG GAGCTGCTGC AGGATCAAGA GAAAGGCCGT
    GCCAGTTTCA CTGCAGTCTC AGAGTTTCTG CTCACACACC CTGTGCTCAG CTTCGGTGTT
    CAGGATGTTT CTCGTGCTCG CCTGCGCCAT ACTGAGGTCC TTCCTCCTGA AGAAGAAAGC
    GAAAGCATGA CAGCAGAAGG AAACCAGGGA ACCTCTGAGT CCAGATCAGG AATTCAGATT
    AAACTCTACT GTGTGCATAC AAAATCACTA CAGGATGTGC AGATCTGGTT TCAGCCCAAT
    CAGGGGGACT CCTCTTCACT CTTCATGCCT CAGTCTGGAT CACAGGATGG GTTCAGTTTT
    TCTGACCCGC TGGCTGATCT GAATGTGGAG GCAATGAGCT CTGACAAGGA GTCTGGCGAT
    AGTGGTTCAC AAAATGACCT GAGTAAGATT CTGCCTCTTC CAAGTCCCGC TGACTTCATG
    AGTCCAGCCC CATCAGCCTT GCCCAAACTC ATGACCCCGG ATGCCTTCAT GACACCCAGC
    GCCTCTATGC CCGCTTCTCC TGGCAGCAGT GCTAGCAGTC TGACCATTGT CACGGCCATG
    AGCAGCTCAG ACAGCGGTGC AAGCAGTTTC GCCAATGCAG GCAGTCCAAG ATCCAACTCC
    ATCCTCATCT CAGGCCTTGG GGAAAACATC CAGGCTTTCG CTCCCCCACT GGTTCTTTCT
    TCATCCCTCC TTCAAGAAGC CTCTCTGCTC TGCGAAGCTG CTCCTTATTC TCACCGACTC
    CCCCTCCCTT ATTTCCCATC TCCTCCTCCG CAGGTTTCCC CTCCAAACCC TCCTCTCTCT
    CTGGATCTCC AGGCAATAGA TCCCATGGTT GTCCCACAGG CGTCTCCAAC AAGAGCTCGC
    TCACCGGATG TCATCTCATC TGCCTCTACT GCAATGTCCC AGGACATACC TGAGATTGCC
    TCCGAGACGC TGCAGAGGGG ACTGTCCGGT GCCAATGCTG ATTCGGGCCC CATCCTGCAC
    TCTGACAGCA TGGCATCAGC CGCATCAATT CTCCACCTGC TATCTCCAAG GGCCCGGAGC
    AGCGCTGAGC ACAGTTTGTT GCCTCTTGAA TTGGGTGCAG CGTCTGTGGA TGGAGAGCAG
    AGACTCAGTA ACACACCATC CCTGCTGGAG ACTGCTCTCT CACAGGAAAA CGCCGGAGCA
    GCCGGAGGAT CCTGTTCTGA CAGCAGTGTG AACCACGCAT GGCCTGCTGC TCCTGATATC
    ACGCGAGAGA CGCGCAACAG CCTGCGAGAC AACGGTTTAG GAGACTGCTC TAGGGAGGAA
    ATAAAAGACA GGCACATTTC CTCCCCCTAC CATAGACGCA CATATCACCT GACTCAGAAC
    GACAGTCAAG ATGCAAGTGC AGAGCAGAGT GATCATGACG ATGAGGTTGC CAGTCTGGCC
    TCGTCCTCAG GGAACTGCGG CCCTCGCTCC TCTCACAGAC TTCCTGTAAA GGACTGGAAA
    ACATCACCCC GCAGCTCCCC TAAGCTCAAG CGGAAGAGCA AGAAAGATGA AGGAGAGTCC
    TCGCAATCTA GACAGATTGA AAGCCAGATG AGCACAGAGG TGCAGGACGA GCTCCTGCAG
    ATGCTGCGGA GCCAGCAGAG GGAGATCGCA GAGCTCCGAC AGAATCAGCT TGATCTTCTG
    CAGAGAGTCA CTAGCCACAT GGACGCAGTG CAGAGCTCCA TCATGGCTCA CATCGAGCAT
    GCCATGTTAG CTCAGCAAGA ACAAGAACAG AGAAGAATGG AGCGAATTCT GGTCGAGGGT
    CAAAGTCGAA ACCAACAGCT CCAGGACCAG CTAGTTCAGC AGCTCGTCCA AACTCTGAAC
    AACAGTCTCT GCAACCGGCT TGAGAAAGTT TTGCGTGAAG AAATGAAGAA AACCGTCCCG
    CAGACCATAT CGAAGAGTCT CGAACCTGTG ACCGGCCAGA TGAACAGCAC CATTGCTGCC
    AAGTTAACTG CAGTAGAAGG AGCACTCAAA GAAAACGTCA CCAAAGTTGT TAAGTCCAAG
    AACACTACAG ATGCCATTGG TCGAGCTGCT GCAGAGGCCA TGCAGGGTCC TATTCAGGCA
    GCCTACAAAG AGACCTTCCA GAGCATTGTC CTGCCTGTGT TTGAGAGAGG CTGTCAGTCC
    ATGTTTCAGC AGATCAATGA CAGCTTCAAA CAGGGAACCA ACGAATACAT CCAGCAGTTG
    GAGACGCACA TCAAAAACAG GAAACAAAGA GACCAGGATA CACGGGATCC TGTCATTGGC
    CAGTTACAGC AGATGATTGA CAGTCTTCAG AGTTCACAAG ACCAGCTGGC GTCTACAGTG
    ACGGCCAGCG TTAGCTCTGA CGTCCAGCAT CAGCTCCACA TGATTGTCGG AAATCTGCAG
    GACTCAATCC TGACACAGGT GCAGCGTATA GTGAAGGGAG AGGTGAGTTT GGCTATGAAG
    GAGCAGCAGG CCGCCGTCAC CTCCAGTATC ATGCAGGCCA TGCGCTCAGC CGCAGGAACA
    CCCGTCCCAT CCGCACATCT GGACTACCAG GCCCAGCAGG CCAGCATATT ACAGCTACTA
    CAGCAGGGCC AGCTTAACGA GGCCTTCCAG CAGGCTTTAT CCGCAGCAGA CCTGAATCTG
    GTCCTGTACG TGTGCGAGAC CATCGACTCC CAACAAGTGT TCGGCCAGCA GCCCTGTCCA
    CTCCACCAGG CCGTCCTTCT GTCCCTCATC CAGCAGCTCT CCACCAACCT GAGCACGCGC
    ACAGAACTCA AGATCAGCTA TCTCGAAGAT GCTGTGATGA ACCTTGACCA CGGAGATCCC
    GTCACCCGAG ACCACATGTC CGCAGTGCTG ACCCAGGTAC GACAGAAGCT CTTCCTGTTC
    CTGCAGCAGG ACGCCCACAG CCCTATGAGC AAGCGTGCAC GGCGCCTCAT GATGATGCTG
    CAGGGCCTGG TCAACCACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44699
    Clone ID Related Accession (Same CDS sequence) XM_009293576.2
    Accession Version XM_009293576.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4101bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product enhancer of mRNA-decapping protein 4 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293576.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCTTCCA GTTCCAGCAT GGACATAGAG GGTGCGACCC AGCACCTTCG GGACATCCTA 
    AAACTGGATC GTCCGGGAAA TTCGGCCGAA GCGTCTTTGG TCGAGAGCCA GAGAAAACCA
    TCTTTCAATG GAGAGCTGAA CGGAGTTCTC GGATCCGATC TCATGGGAGC AGCAATGGTG
    GAGCCGACGG TGCACAACTC AGACAAACTT AATGGACAGG ACACACAGAC CATCTGTCTT
    TCAGGAGATG ACGGGTCCAT TTGTGTCCCA ATCAGAGCGA ATATTGTGGA AATTGTGTCC
    AGCCGTGACT CTAGTATTGA CAGTAAGGCT CGTGGAAGCA ACAAGGTTAA AATTCAGCCA
    GTGGCCAAAT ATGACTGGGA GCACAAGTAC TACTATGGCA ATTTAATAGC CGTGTCCAAC
    ACGTACTTGG CTTATGCTAT CAGAGGTGCA AACAATCATT CAATGATTCG TGTGCTGCAC
    CTGAGCTCTA CAGAGCGCTC TCTGCTGAAG GGCTTCACCG GGGCTGTCAC AGACCTTGCC
    TTTGCCCATC TGGACTCCAC CCTGTTGGGA TGTGTGGACG AAGCCGGAAA CATGTTCATT
    TGGCAGCTTA CAAGCCACAG CAGCAAAATA CAAGACGAAG TGATTGTTCA CATCCGCAGA
    CCTGAGGACA CTCCACTAAA CTCTAACCGC AGACTCATCT GGTGTCCCTT CATCCCGGAG
    GACAATGACG AGAGCCCAGA GGATGCATGT CAGACATTAG CATTGCTTCA TGAAGACAGA
    GCTGAGGTTT GGGACCTCGA CATCCTCCGA TCAAATAACA GCTCATGGCC TGTGGACGCC
    ACTGAATTGA AAGAGGGTTT CATCACCATT AGAGGGCATG CAGCTCGCAT AAGTGAGGGA
    GCTCTGTCCC CTGATGGTAC GGTTTTGGCC ACAGCTAGTC ATGATGGATA TGTCAAATTT
    TGGCAAATTT ACATTGAAGG ACAGGACCAA CCCCGATGCC TGCATGAATG GCAGCCGCAT
    AATGGACAGC CTCTATCTTG CCTGCTGTTT TGTGACAACC ACAAGAAGCA AGATCCAGAC
    GTTCCATTCT GGCGCTTCCT GATTACTGGA GCTGATCAGA ACCAGGAGCT GAAGATGTGG
    TGCACAGTAT CATGGACCTG CCTACAAACC ATCAGGTTTT CTCCAGATCC ATTTAACTGC
    GGTGTGCTAC CCAGCCTGAA GGCCAGTTTG GACCTTTCTG CAGAATTTCT CATTCTTAGT
    GATGTTCAAA GGAAGGTATT GTATGTCATG GAGCTGCTGC AGGATCAAGA GAAAGGCCGT
    GCCAGTTTCA CTGCAGTCTC AGAGTTTCTG CTCACACACC CTGTGCTCAG CTTCGGTGTT
    CAGGATGTTT CTCGTGCTCG CCTGCGCCAT ACTGAGGTCC TTCCTCCTGA AGAAGAAAGC
    GAAAGCATGA CAGCAGAAGG AAACCAGGGA ACCTCTGAGT CCAGATCAGG AATTCAGATT
    AAACTCTACT GTGTGCATAC AAAATCACTA CAGGATGTGC AGATCTGGTT TCAGCCCAAT
    CAGGGGGACT CCTCTTCACT CTTCATGCCT CAGTCTGGAT CACAGGATGG GTTCAGTTTT
    TCTGACCCGC TGGCTGATCT GAATGTGGAG GCAATGAGCT CTGACAAGGA GTCTGGCGAT
    AGTGGTTCAC AAAATGACCT GAGTAAGATT CTGCCTCTTC CAAGTCCCGC TGACTTCATG
    AGTCCAGCCC CATCAGCCTT GCCCAAACTC ATGACCCCGG ATGCCTTCAT GACACCCAGC
    GCCTCTATGC CCGCTTCTCC TGGCAGCAGT GCTAGCAGTC TGACCATTGT CACGGCCATG
    AGCAGCTCAG ACAGCGGTGC AAGCAGTTTC GCCAATGCAG GCAGTCCAAG ATCCAACTCC
    ATCCTCATCT CAGGCCTTGG GGAAAACATC CAGGTTTCCC CTCCAAACCC TCCTCTCTCT
    CTGGATCTCC AGGCAATAGA TCCCATGGTT GTCCCACAGG CGTCTCCAAC AAGAGCTCGC
    TCACCGGATG TCATCTCATC TGCCTCTACT GCAATGTCCC AGGACATACC TGAGATTGCC
    TCCGAGACGC TGCAGAGGGG ACTGTCCGGT GCCAATGCTG ATTCGGGCCC CATCCTGCAC
    TCTGACAGCA TGGCATCAGC CGCATCAATT CTCCACCTGC TATCTCCAAG GGCCCGGAGC
    AGCGCTGAGC ACAGTTTGTT GCCTCTTGAA TTGGGTGCAG CGTCTGTGGA TGGAGAGCAG
    AGACTCAGTA ACACACCATC CCTGCTGGAG ACTGCTCTCT CACAGGAAAA CGCCGGAGCA
    GCCGGAGGAT CCTGTTCTGA CAGCAGTGTG AACCACGCAT GGCCTGCTGC TCCTGATATC
    ACGCGAGAGA CGCGCAACAG CCTGCGAGAC AACGGTTTAG GAGACTGCTC TAGGGAGGAA
    ATAAAAGACA GGCACATTTC CTCCCCCTAC CATAGACGCA CATATCACCT GACTCAGAAC
    GACAGTCAAG ATGCAAGTGC AGAGCAGAGT GATCATGACG ATGAGGTTGC CAGTCTGGCC
    TCGTCCTCAG GGAACTGCGG CCCTCGCTCC TCTCACAGAC TTCCTGTAAA GGACTGGAAA
    ACATCACCCC GCAGCTCCCC TAAGCTCAAG CGGAAGAGCA AGAAAGATGA AGGAGAGTCC
    TCGCAATCTA GACAGATTGA AAGCCAGATG AGCACAGAGG TGCAGGACGA GCTCCTGCAG
    ATGCTGCGGA GCCAGCAGAG GGAGATCGCA GAGCTCCGAC AGAATCAGCT TGATCTTCTG
    CAGAGAGTCA CTAGCCACAT GGACGCAGTG CAGAGCTCCA TCATGGCTCA CATCGAGCAT
    GCCATGTTAG CTCAGCAAGA ACAAGAACAG AGAAGAATGG AGCGAATTCT GGTCGAGGGT
    CAAAGTCGAA ACCAACAGCT CCAGGACCAG CTAGTTCAGC AGCTCGTCCA AACTCTGAAC
    AACAGTCTCT GCAACCGGCT TGAGAAAGTT TTGCGTGAAG AAATGAAGAA AACCGTCCCG
    CAGACCATAT CGAAGAGTCT CGAACCTGTG ACCGGCCAGA TGAACAGCAC CATTGCTGCC
    AAGTTAACTG CAGTAGAAGG AGCACTCAAA GAAAACGTCA CCAAAGTTGT TAAGTCCAAG
    AACACTACAG ATGCCATTGG TCGAGCTGCT GCAGAGGCCA TGCAGGGTCC TATTCAGGCA
    GCCTACAAAG AGACCTTCCA GAGCATTGTC CTGCCTGTGT TTGAGAGAGG CTGTCAGTCC
    ATGTTTCAGC AGATCAATGA CAGCTTCAAA CAGGGAACCA ACGAATACAT CCAGCAGTTG
    GAGACGCACA TCAAAAACAG GAAACAAAGA GACCAGGATA CACGGGATCC TGTCATTGGC
    CAGTTACAGC AGATGATTGA CAGTCTTCAG AGTTCACAAG ACCAGCTGGC GTCTACAGTG
    ACGGCCAGCG TTAGCTCTGA CGTCCAGCAT CAGCTCCACA TGATTGTCGG AAATCTGCAG
    GACTCAATCC TGACACAGGT GCAGCGTATA GTGAAGGGAG AGGTGAGTTT GGCTATGAAG
    GAGCAGCAGG CCGCCGTCAC CTCCAGTATC ATGCAGGCCA TGCGCTCAGC CGCAGGAACA
    CCCGTCCCAT CCGCACATCT GGACTACCAG GCCCAGCAGG CCAGCATATT ACAGCTACTA
    CAGCAGGGCC AGCTTAACGA GGCCTTCCAG CAGGCTTTAT CCGCAGCAGA CCTGAATCTG
    GTCCTGTACG TGTGCGAGAC CATCGACTCC CAACAAGTGT TCGGCCAGCA GCCCTGTCCA
    CTCCACCAGG CCGTCCTTCT GTCCCTCATC CAGCAGCTCT CCACCAACCT GAGCACGCGC
    ACAGAACTCA AGATCAGCTA TCTCGAAGAT GCTGTGATGA ACCTTGACCA CGGAGATCCC
    GTCACCCGAG ACCACATGTC CGCAGTGCTG ACCCAGGTAC GACAGAAGCT CTTCCTGTTC
    CTGCAGCAGG ACGCCCACAG CCCTATGAGC AAGCGTGCAC GGCGCCTCAT GATGATGCTG
    CAGGGCCTGG TCAACCACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009291851.1
    CDS132..4232
    Translation

    Target ORF information:

    RefSeq Version XM_009293576.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio enhancer of mRNA decapping 4 (edc4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009293576.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGCTTCCA GTTCCAGCAT GGACATAGAG GGTGCGACCC AGCACCTTCG GGACATCCTA 
    AAACTGGATC GTCCGGGAAA TTCGGCCGAA GCGTCTTTGG TCGAGAGCCA GAGAAAACCA
    TCTTTCAATG GAGAGCTGAA CGGAGTTCTC GGATCCGATC TCATGGGAGC AGCAATGGTG
    GAGCCGACGG TGCACAACTC AGACAAACTT AATGGACAGG ACACACAGAC CATCTGTCTT
    TCAGGAGATG ACGGGTCCAT TTGTGTCCCA ATCAGAGCGA ATATTGTGGA AATTGTGTCC
    AGCCGTGACT CTAGTATTGA CAGTAAGGCT CGTGGAAGCA ACAAGGTTAA AATTCAGCCA
    GTGGCCAAAT ATGACTGGGA GCACAAGTAC TACTATGGCA ATTTAATAGC CGTGTCCAAC
    ACGTACTTGG CTTATGCTAT CAGAGGTGCA AACAATCATT CAATGATTCG TGTGCTGCAC
    CTGAGCTCTA CAGAGCGCTC TCTGCTGAAG GGCTTCACCG GGGCTGTCAC AGACCTTGCC
    TTTGCCCATC TGGACTCCAC CCTGTTGGGA TGTGTGGACG AAGCCGGAAA CATGTTCATT
    TGGCAGCTTA CAAGCCACAG CAGCAAAATA CAAGACGAAG TGATTGTTCA CATCCGCAGA
    CCTGAGGACA CTCCACTAAA CTCTAACCGC AGACTCATCT GGTGTCCCTT CATCCCGGAG
    GACAATGACG AGAGCCCAGA GGATGCATGT CAGACATTAG CATTGCTTCA TGAAGACAGA
    GCTGAGGTTT GGGACCTCGA CATCCTCCGA TCAAATAACA GCTCATGGCC TGTGGACGCC
    ACTGAATTGA AAGAGGGTTT CATCACCATT AGAGGGCATG CAGCTCGCAT AAGTGAGGGA
    GCTCTGTCCC CTGATGGTAC GGTTTTGGCC ACAGCTAGTC ATGATGGATA TGTCAAATTT
    TGGCAAATTT ACATTGAAGG ACAGGACCAA CCCCGATGCC TGCATGAATG GCAGCCGCAT
    AATGGACAGC CTCTATCTTG CCTGCTGTTT TGTGACAACC ACAAGAAGCA AGATCCAGAC
    GTTCCATTCT GGCGCTTCCT GATTACTGGA GCTGATCAGA ACCAGGAGCT GAAGATGTGG
    TGCACAGTAT CATGGACCTG CCTACAAACC ATCAGGTTTT CTCCAGATCC ATTTAACTGC
    GGTGTGCTAC CCAGCCTGAA GGCCAGTTTG GACCTTTCTG CAGAATTTCT CATTCTTAGT
    GATGTTCAAA GGAAGGTATT GTATGTCATG GAGCTGCTGC AGGATCAAGA GAAAGGCCGT
    GCCAGTTTCA CTGCAGTCTC AGAGTTTCTG CTCACACACC CTGTGCTCAG CTTCGGTGTT
    CAGGATGTTT CTCGTGCTCG CCTGCGCCAT ACTGAGGTCC TTCCTCCTGA AGAAGAAAGC
    GAAAGCATGA CAGCAGAAGG AAACCAGGGA ACCTCTGAGT CCAGATCAGG AATTCAGATT
    AAACTCTACT GTGTGCATAC AAAATCACTA CAGGATGTGC AGATCTGGTT TCAGCCCAAT
    CAGGGGGACT CCTCTTCACT CTTCATGCCT CAGTCTGGAT CACAGGATGG GTTCAGTTTT
    TCTGACCCGC TGGCTGATCT GAATGTGGAG GCAATGAGCT CTGACAAGGA GTCTGGCGAT
    AGTGGTTCAC AAAATGACCT GAGTAAGATT CTGCCTCTTC CAAGTCCCGC TGACTTCATG
    AGTCCAGCCC CATCAGCCTT GCCCAAACTC ATGACCCCGG ATGCCTTCAT GACACCCAGC
    GCCTCTATGC CCGCTTCTCC TGGCAGCAGT GCTAGCAGTC TGACCATTGT CACGGCCATG
    AGCAGCTCAG ACAGCGGTGC AAGCAGTTTC GCCAATGCAG GCAGTCCAAG ATCCAACTCC
    ATCCTCATCT CAGGCCTTGG GGAAAACATC CAGGTTTCCC CTCCAAACCC TCCTCTCTCT
    CTGGATCTCC AGGCAATAGA TCCCATGGTT GTCCCACAGG CGTCTCCAAC AAGAGCTCGC
    TCACCGGATG TCATCTCATC TGCCTCTACT GCAATGTCCC AGGACATACC TGAGATTGCC
    TCCGAGACGC TGCAGAGGGG ACTGTCCGGT GCCAATGCTG ATTCGGGCCC CATCCTGCAC
    TCTGACAGCA TGGCATCAGC CGCATCAATT CTCCACCTGC TATCTCCAAG GGCCCGGAGC
    AGCGCTGAGC ACAGTTTGTT GCCTCTTGAA TTGGGTGCAG CGTCTGTGGA TGGAGAGCAG
    AGACTCAGTA ACACACCATC CCTGCTGGAG ACTGCTCTCT CACAGGAAAA CGCCGGAGCA
    GCCGGAGGAT CCTGTTCTGA CAGCAGTGTG AACCACGCAT GGCCTGCTGC TCCTGATATC
    ACGCGAGAGA CGCGCAACAG CCTGCGAGAC AACGGTTTAG GAGACTGCTC TAGGGAGGAA
    ATAAAAGACA GGCACATTTC CTCCCCCTAC CATAGACGCA CATATCACCT GACTCAGAAC
    GACAGTCAAG ATGCAAGTGC AGAGCAGAGT GATCATGACG ATGAGGTTGC CAGTCTGGCC
    TCGTCCTCAG GGAACTGCGG CCCTCGCTCC TCTCACAGAC TTCCTGTAAA GGACTGGAAA
    ACATCACCCC GCAGCTCCCC TAAGCTCAAG CGGAAGAGCA AGAAAGATGA AGGAGAGTCC
    TCGCAATCTA GACAGATTGA AAGCCAGATG AGCACAGAGG TGCAGGACGA GCTCCTGCAG
    ATGCTGCGGA GCCAGCAGAG GGAGATCGCA GAGCTCCGAC AGAATCAGCT TGATCTTCTG
    CAGAGAGTCA CTAGCCACAT GGACGCAGTG CAGAGCTCCA TCATGGCTCA CATCGAGCAT
    GCCATGTTAG CTCAGCAAGA ACAAGAACAG AGAAGAATGG AGCGAATTCT GGTCGAGGGT
    CAAAGTCGAA ACCAACAGCT CCAGGACCAG CTAGTTCAGC AGCTCGTCCA AACTCTGAAC
    AACAGTCTCT GCAACCGGCT TGAGAAAGTT TTGCGTGAAG AAATGAAGAA AACCGTCCCG
    CAGACCATAT CGAAGAGTCT CGAACCTGTG ACCGGCCAGA TGAACAGCAC CATTGCTGCC
    AAGTTAACTG CAGTAGAAGG AGCACTCAAA GAAAACGTCA CCAAAGTTGT TAAGTCCAAG
    AACACTACAG ATGCCATTGG TCGAGCTGCT GCAGAGGCCA TGCAGGGTCC TATTCAGGCA
    GCCTACAAAG AGACCTTCCA GAGCATTGTC CTGCCTGTGT TTGAGAGAGG CTGTCAGTCC
    ATGTTTCAGC AGATCAATGA CAGCTTCAAA CAGGGAACCA ACGAATACAT CCAGCAGTTG
    GAGACGCACA TCAAAAACAG GAAACAAAGA GACCAGGATA CACGGGATCC TGTCATTGGC
    CAGTTACAGC AGATGATTGA CAGTCTTCAG AGTTCACAAG ACCAGCTGGC GTCTACAGTG
    ACGGCCAGCG TTAGCTCTGA CGTCCAGCAT CAGCTCCACA TGATTGTCGG AAATCTGCAG
    GACTCAATCC TGACACAGGT GCAGCGTATA GTGAAGGGAG AGGTGAGTTT GGCTATGAAG
    GAGCAGCAGG CCGCCGTCAC CTCCAGTATC ATGCAGGCCA TGCGCTCAGC CGCAGGAACA
    CCCGTCCCAT CCGCACATCT GGACTACCAG GCCCAGCAGG CCAGCATATT ACAGCTACTA
    CAGCAGGGCC AGCTTAACGA GGCCTTCCAG CAGGCTTTAT CCGCAGCAGA CCTGAATCTG
    GTCCTGTACG TGTGCGAGAC CATCGACTCC CAACAAGTGT TCGGCCAGCA GCCCTGTCCA
    CTCCACCAGG CCGTCCTTCT GTCCCTCATC CAGCAGCTCT CCACCAACCT GAGCACGCGC
    ACAGAACTCA AGATCAGCTA TCTCGAAGAT GCTGTGATGA ACCTTGACCA CGGAGATCCC
    GTCACCCGAG ACCACATGTC CGCAGTGCTG ACCCAGGTAC GACAGAAGCT CTTCCTGTTC
    CTGCAGCAGG ACGCCCACAG CCCTATGAGC AAGCGTGCAC GGCGCCTCAT GATGATGCTG
    CAGGGCCTGG TCAACCACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.