UNKL cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	UNKL
Entrez Gene ID	565405
Full Name	unkempt family zinc finger-like
Synonyms	si:ch211-66h3.3
General protein information	Preferred Names unkempt family zinc finger-like Names RING finger protein unkempt-like putative E3 ubiquitin-protein ligase UNKL unkempt homolog-like
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005164231.4	XP_005164288.1	putative E3 ubiquitin-protein ligase UNKL isoform X1
XM_005164232.4	XP_005164289.1	putative E3 ubiquitin-protein ligase UNKL isoform X2
XM_005164231.3	XP_005164288.1	putative E3 ubiquitin-protein ligase UNKL isoform X1
XM_005164232.3	XP_005164289.1	putative E3 ubiquitin-protein ligase UNKL isoform X2

Pathways from BioSystems

Homologies

	LOC100429104	XP_002802402.1
Mus musculus (house mouse)	Unkl	NP_001183953.1
Homo sapiens (human)	UNKL	NP_001180317.2
Bos taurus (cattle)	UNKL	XP_005224655.1
Danio rerio (zebrafish)	unkl	XP_005164288.1
Gallus gallus (chicken)	UNKL	XP_004945341.1
Canis lupus familiaris (dog)	UNKL	XP_005622153.1
Rattus norvegicus (Norway rat)	Unkl	XP_220238.3
Xenopus tropicalis (tropical clawed frog)	unkl	NP_001116915.1

Gene Ontology

The following UNKL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the UNKL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa08715	XM_005164231.4 Latest version!	Danio rerio unkempt family zinc finger-like (unkl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa08717	XM_005164232.4 Latest version!	Danio rerio unkempt family zinc finger-like (unkl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
View Old Accession Versions >>
ODa08715	XM_005164231.3	Danio rerio unkempt family zinc finger-like (unkl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa08717	XM_005164232.3	Danio rerio unkempt family zinc finger-like (unkl), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

UNKL ( XM_005164231.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005164288.1 Danio rerio unkempt family zinc finger-like (unkl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08715
Clone ID Related Accession (Same CDS sequence)	XM_005164231.4 , XM_005164231.3
Accession Version	XM_005164231.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	putative E3 ubiquitin-protein ligase UNKL isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164231.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTCGG TTTCGAAAAC GGCGGCGAGC GCTTCTCCTC AAACCGAGAA ACCAACCCAC 
TATACATATT TGAAGGAGTT CAGGACCGAG CAGTGCCCAC TGTTTTTGCA GCACAAATGC 
ACACAGCATA GACCCTTTAC ATGTTTCCAC TGGCATTTTC TAAACCAGCG AAGACGGAGA 
CCCATACGGA GAAGAGACGG GACTTTTAAT TACAGCCCAG ATGTGTATTG CACGAAGTAT 
GATGAAACAA CAGGGATATG TCCCGACGGA GACGATTGCC CTTATTTACA CCGGACCACC 
GGTGACACGG AGCGAAAGTA TCATTTACGC TACTACAAAA CCGGCACCTG CATCCATGAG 
ACAGATGCCC GTGGACATTG TGTGAAGAAT GGCCTCCACT GCGCTTTCGC TCATGGACCA 
CATGACCTAC GGCCACCAGT TTATGACATC AGAGAGATCC AGGCACAAGA AGCTCTGCAG 
AATGGCCAGT TGGGATCTGG GGAGGGAATT CCTGACCTGC AGCCGGGTGT GCTGGCCAGC 
CAGGCTATGA TTGAGAAGAC CCTGAATGAG GACCCGCGCT GGCAAGACAC CAATTTCGTT 
TTGGCCAACT ATAAGACCGA TCAGTGTACC AAGCCTCCAC GTCTATGCAG GCAGGGCTAT 
GCTTGTCCCC ACTATCACAA CAGCAGAGAT AGAAGAAGAA ATCCCAGAAA GTTCAAATAC 
AGATCGACGC CCTGCCCGAG TGTGAAGCAT GGAGATGAAT GGGGCGAACC CTCCAAATGT 
GAGAGTGGAG ACAGCTGCCA GTACTGCCAC TCTCGCACCG AACAGCAGTT CCACCCAGAG 
ATTTACAAGT CAACTAAATG CAATGACATG AGGCAAACCG GATACTGTCC AAGGGGCCCG 
TTCTGTGCTT TTGCACATGT GGAAAGAATT CCTTCCACAG AAGAGACCAT GAGCACGTTG 
CTCTCAGCAA TGCAGTCTGG TTCTCAGTCT AAGCTGGGCT CTCAGTACCC AGAGTGTCCA 
GTCAGTGAGT GGAGCAACAA CACCAATTCC ATCACCAGCA GCGCTAGCAA CAACGGCCAG 
AGCGGAAACG TATCATGCTC TAATAGTCCA ACTGTAACAC CAAGCTCAGG GAGCAACAGT 
TCTTTGTCCC CAATTGGACC CATCGGCAGG CCAAAATGCT TTACTAATGG TAGCTTATGT 
TCAGAGTCCA CAACATCCAG TGTTTCCTCT CCAACGTCTA ACTATCCCAA AGCGCCGGGT 
TTTGAACGGG AGGATCAGGC AAAGAACCTG AAGAGCCAGT CTGGGGAGAA TAATCAGAAG 
TTAATGGACC AAGACAAACA GGCTCATGGC AGCGTGTTCT CCGTGGTGAA CCCACTAGCG 
TCCAGCATAA CATCAAGCAT AACGTCCAGC TTGGCCTCCA GCATCGGATC TGACAGTTCA 
TCGCCCACCA CTTTATCAAC CATGAATGCA AAAGCCACGC CTTTCTATCC GGGCAGCAAT 
ACTGTGGAGT CAGTAATAGG ATCTGCACTT GACCTAAATT TTAGTGACAT CAATGTTGCC 
TCTCTCGACA AAGAGCTAGA GGACCAAGAC AACAATGGCC TTGCTTTAAC AAGTCAGAGG 
TTATTAGGAG GCTCTGCTCC AGTTAATATT CCTGGCTCTC TGGCTCGGTC TTCCTCTCTG 
CACTCGTCAT CGTCTCTGTC TGCATCTCCG CTCAGCTCCC TGTCTCAATC CCTGTCCCAG 
TCACTCCTCT CTGGAATGGC CTCCCAACAG AGCCAGACAC AGCCACCACC TGTTAAAACC 
GAGCATGGCC TTCTGGGAAC ACCCACATCT ACACAGAACT CCTTAGGTCT GAATGGCGGA 
GCAGGGGGTA TATGGGACTT TATGAGTGGC AGCTTCTCTC CCAGCCCGTC TCCAGTGTTC 
AGCAGCCTTA CTTCCAACAC GGTCGGCTCC AGCAGCGCAG ACATCGGCCG ACTCCTCCGA 
GAACTGGATG AAGCCAAGCG TAAGATCAAG CAGTGGGAGG AGGCCTGGCA CCAAGTCAAA 
CAGGCCTGTG AAGCATGGCA GAAAGACGCT CACGAAGCGA AAGAACAAGC AAAATCGGCA 
GAAGCAGAGC GTCAGCTGGC GGAGCAGAAG CGGGAGGACG CGGAGCGCAA GCTGAAGGAA 
CTGCAGGGAG ACTTTGACGC TTTGTGTCGT TCGCCCAACA CACCTCTTCT GCGCAGCTAC 
GGCGAGCTGG AGCAGCTCCC CCTGCCAAAG CTCCGCTCCA TCCAGAGCCA GCTACGCTCT 
GACCTAGACC TCATAGAAGG GGTAATATAT CAGCTTCAGT CAAAGAAATG TATAGTTTGC 
CAAAAGCATG ATCGTTCCAT TGTCCTGCAG CCTTGCCAAC ATTATGTACT TTGTCAGAAC 
TGTGCGTCTG GTAAAAAGGA ATGTCCTTAC TGTAAGACGA AAATATCCAA GTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164288.1
CDS	70..2526
Translation	MPSVSKTAASASPQTEKPTHYTYLKEFRTEQCPLFLQHKCTQHRPFTCFHWHFLNQRRRRPIRRRDGTFNYSPDVYCTKYDETTGICPDGDDCPYLHRTTGDTERKYHLRYYKTGTCIHETDARGHCVKNGLHCAFAHGPHDLRPPVYDIREIQAQEALQNGQLGSGEGIPDLQPGVLASQAMIEKTLNEDPRWQDTNFVLANYKTDQCTKPPRLCRQGYACPHYHNSRDRRRNPRKFKYRSTPCPSVKHGDEWGEPSKCESGDSCQYCHSRTEQQFHPEIYKSTKCNDMRQTGYCPRGPFCAFAHVERIPSTEETMSTLLSAMQSGSQSKLGSQYPECPVSEWSNNTNSITSSASNNGQSGNVSCSNSPTVTPSSGSNSSLSPIGPIGRPKCFTNGSLCSESTTSSVSSPTSNYPKAPGFEREDQAKNLKSQSGENNQKLMDQDKQAHGSVFSVVNPLASSITSSITSSLASSIGSDSSSPTTLSTMNAKATPFYPGSNTVESVIGSALDLNFSDINVASLDKELEDQDNNGLALTSQRLLGGSAPVNIPGSLARSSSLHSSSSLSASPLSSLSQSLSQSLLSGMASQQSQTQPPPVKTEHGLLGTPTSTQNSLGLNGGAGGIWDFMSGSFSPSPSPVFSSLTSNTVGSSSADIGRLLRELDEAKRKIKQWEEAWHQVKQACEAWQKDAHEAKEQAKSAEAERQLAEQKREDAERKLKELQGDFDALCRSPNTPLLRSYGELEQLPLPKLRSIQSQLRSDLDLIEGVIYQLQSKKCIVCQKHDRSIVLQPCQHYVLCQNCASGKKECPYCKTKISKW

Target ORF information:

RefSeq Version	XM_005164231.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio unkempt family zinc finger-like (unkl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164231.4

ORF Insert Sequence:

ATGCCGTCGG TTTCGAAAAC GGCGGCGAGC GCTTCTCCTC AAACCGAGAA ACCAACCCAC 
TATACATATT TGAAGGAGTT CAGGACCGAG CAGTGCCCAC TGTTTTTGCA GCACAAATGC 
ACACAGCATA GACCCTTTAC ATGTTTCCAC TGGCATTTTC TAAACCAGCG AAGACGGAGA 
CCCATACGGA GAAGAGACGG GACTTTTAAT TACAGCCCAG ATGTGTATTG CACGAAGTAT 
GATGAAACAA CAGGGATATG TCCCGACGGA GACGATTGCC CTTATTTACA CCGGACCACC 
GGTGACACGG AGCGAAAGTA TCATTTACGC TACTACAAAA CCGGCACCTG CATCCATGAG 
ACAGATGCCC GTGGACATTG TGTGAAGAAT GGCCTCCACT GCGCTTTCGC TCATGGACCA 
CATGACCTAC GGCCACCAGT TTATGACATC AGAGAGATCC AGGCACAAGA AGCTCTGCAG 
AATGGCCAGT TGGGATCTGG GGAGGGAATT CCTGACCTGC AGCCGGGTGT GCTGGCCAGC 
CAGGCTATGA TTGAGAAGAC CCTGAATGAG GACCCGCGCT GGCAAGACAC CAATTTCGTT 
TTGGCCAACT ATAAGACCGA TCAGTGTACC AAGCCTCCAC GTCTATGCAG GCAGGGCTAT 
GCTTGTCCCC ACTATCACAA CAGCAGAGAT AGAAGAAGAA ATCCCAGAAA GTTCAAATAC 
AGATCGACGC CCTGCCCGAG TGTGAAGCAT GGAGATGAAT GGGGCGAACC CTCCAAATGT 
GAGAGTGGAG ACAGCTGCCA GTACTGCCAC TCTCGCACCG AACAGCAGTT CCACCCAGAG 
ATTTACAAGT CAACTAAATG CAATGACATG AGGCAAACCG GATACTGTCC AAGGGGCCCG 
TTCTGTGCTT TTGCACATGT GGAAAGAATT CCTTCCACAG AAGAGACCAT GAGCACGTTG 
CTCTCAGCAA TGCAGTCTGG TTCTCAGTCT AAGCTGGGCT CTCAGTACCC AGAGTGTCCA 
GTCAGTGAGT GGAGCAACAA CACCAATTCC ATCACCAGCA GCGCTAGCAA CAACGGCCAG 
AGCGGAAACG TATCATGCTC TAATAGTCCA ACTGTAACAC CAAGCTCAGG GAGCAACAGT 
TCTTTGTCCC CAATTGGACC CATCGGCAGG CCAAAATGCT TTACTAATGG TAGCTTATGT 
TCAGAGTCCA CAACATCCAG TGTTTCCTCT CCAACGTCTA ACTATCCCAA AGCGCCGGGT 
TTTGAACGGG AGGATCAGGC AAAGAACCTG AAGAGCCAGT CTGGGGAGAA TAATCAGAAG 
TTAATGGACC AAGACAAACA GGCTCATGGC AGCGTGTTCT CCGTGGTGAA CCCACTAGCG 
TCCAGCATAA CATCAAGCAT AACGTCCAGC TTGGCCTCCA GCATCGGATC TGACAGTTCA 
TCGCCCACCA CTTTATCAAC CATGAATGCA AAAGCCACGC CTTTCTATCC GGGCAGCAAT 
ACTGTGGAGT CAGTAATAGG ATCTGCACTT GACCTAAATT TTAGTGACAT CAATGTTGCC 
TCTCTCGACA AAGAGCTAGA GGACCAAGAC AACAATGGCC TTGCTTTAAC AAGTCAGAGG 
TTATTAGGAG GCTCTGCTCC AGTTAATATT CCTGGCTCTC TGGCTCGGTC TTCCTCTCTG 
CACTCGTCAT CGTCTCTGTC TGCATCTCCG CTCAGCTCCC TGTCTCAATC CCTGTCCCAG 
TCACTCCTCT CTGGAATGGC CTCCCAACAG AGCCAGACAC AGCCACCACC TGTTAAAACC 
GAGCATGGCC TTCTGGGAAC ACCCACATCT ACACAGAACT CCTTAGGTCT GAATGGCGGA 
GCAGGGGGTA TATGGGACTT TATGAGTGGC AGCTTCTCTC CCAGCCCGTC TCCAGTGTTC 
AGCAGCCTTA CTTCCAACAC GGTCGGCTCC AGCAGCGCAG ACATCGGCCG ACTCCTCCGA 
GAACTGGATG AAGCCAAGCG TAAGATCAAG CAGTGGGAGG AGGCCTGGCA CCAAGTCAAA 
CAGGCCTGTG AAGCATGGCA GAAAGACGCT CACGAAGCGA AAGAACAAGC AAAATCGGCA 
GAAGCAGAGC GTCAGCTGGC GGAGCAGAAG CGGGAGGACG CGGAGCGCAA GCTGAAGGAA 
CTGCAGGGAG ACTTTGACGC TTTGTGTCGT TCGCCCAACA CACCTCTTCT GCGCAGCTAC 
GGCGAGCTGG AGCAGCTCCC CCTGCCAAAG CTCCGCTCCA TCCAGAGCCA GCTACGCTCT 
GACCTAGACC TCATAGAAGG GGTAATATAT CAGCTTCAGT CAAAGAAATG TATAGTTTGC 
CAAAAGCATG ATCGTTCCAT TGTCCTGCAG CCTTGCCAAC ATTATGTACT TTGTCAGAAC 
TGTGCGTCTG GTAAAAAGGA ATGTCCTTAC TGTAAGACGA AAATATCCAA GTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNKL ( XM_005164231.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005164288.1 Danio rerio unkempt family zinc finger-like (unkl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa08715
Clone ID Related Accession (Same CDS sequence)	XM_005164231.4 , XM_005164231.3
Accession Version	XM_005164231.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2457bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	putative E3 ubiquitin-protein ligase UNKL isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164231.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTCGG TTTCGAAAAC GGCGGCGAGC GCTTCTCCTC AAACCGAGAA ACCAACCCAC 
TATACATATT TGAAGGAGTT CAGGACCGAG CAGTGCCCAC TGTTTTTGCA GCACAAATGC 
ACACAGCATA GACCCTTTAC ATGTTTCCAC TGGCATTTTC TAAACCAGCG AAGACGGAGA 
CCCATACGGA GAAGAGACGG GACTTTTAAT TACAGCCCAG ATGTGTATTG CACGAAGTAT 
GATGAAACAA CAGGGATATG TCCCGACGGA GACGATTGCC CTTATTTACA CCGGACCACC 
GGTGACACGG AGCGAAAGTA TCATTTACGC TACTACAAAA CCGGCACCTG CATCCATGAG 
ACAGATGCCC GTGGACATTG TGTGAAGAAT GGCCTCCACT GCGCTTTCGC TCATGGACCA 
CATGACCTAC GGCCACCAGT TTATGACATC AGAGAGATCC AGGCACAAGA AGCTCTGCAG 
AATGGCCAGT TGGGATCTGG GGAGGGAATT CCTGACCTGC AGCCGGGTGT GCTGGCCAGC 
CAGGCTATGA TTGAGAAGAC CCTGAATGAG GACCCGCGCT GGCAAGACAC CAATTTCGTT 
TTGGCCAACT ATAAGACCGA TCAGTGTACC AAGCCTCCAC GTCTATGCAG GCAGGGCTAT 
GCTTGTCCCC ACTATCACAA CAGCAGAGAT AGAAGAAGAA ATCCCAGAAA GTTCAAATAC 
AGATCGACGC CCTGCCCGAG TGTGAAGCAT GGAGATGAAT GGGGCGAACC CTCCAAATGT 
GAGAGTGGAG ACAGCTGCCA GTACTGCCAC TCTCGCACCG AACAGCAGTT CCACCCAGAG 
ATTTACAAGT CAACTAAATG CAATGACATG AGGCAAACCG GATACTGTCC AAGGGGCCCG 
TTCTGTGCTT TTGCACATGT GGAAAGAATT CCTTCCACAG AAGAGACCAT GAGCACGTTG 
CTCTCAGCAA TGCAGTCTGG TTCTCAGTCT AAGCTGGGCT CTCAGTACCC AGAGTGTCCA 
GTCAGTGAGT GGAGCAACAA CACCAATTCC ATCACCAGCA GCGCTAGCAA CAACGGCCAG 
AGCGGAAACG TATCATGCTC TAATAGTCCA ACTGTAACAC CAAGCTCAGG GAGCAACAGT 
TCTTTGTCCC CAATTGGACC CATCGGCAGG CCAAAATGCT TTACTAATGG TAGCTTATGT 
TCAGAGTCCA CAACATCCAG TGTTTCCTCT CCAACGTCTA ACTATCCCAA AGCGCCGGGT 
TTTGAACGGG AGGATCAGGC AAAGAACCTG AAGAGCCAGT CTGGGGAGAA TAATCAGAAG 
TTAATGGACC AAGACAAACA GGCTCATGGC AGCGTGTTCT CCGTGGTGAA CCCACTAGCG 
TCCAGCATAA CATCAAGCAT AACGTCCAGC TTGGCCTCCA GCATCGGATC TGACAGTTCA 
TCGCCCACCA CTTTATCAAC CATGAATGCA AAAGCCACGC CTTTCTATCC GGGCAGCAAT 
ACTGTGGAGT CAGTAATAGG ATCTGCACTT GACCTAAATT TTAGTGACAT CAATGTTGCC 
TCTCTCGACA AAGAGCTAGA GGACCAAGAC AACAATGGCC TTGCTTTAAC AAGTCAGAGG 
TTATTAGGAG GCTCTGCTCC AGTTAATATT CCTGGCTCTC TGGCTCGGTC TTCCTCTCTG 
CACTCGTCAT CGTCTCTGTC TGCATCTCCG CTCAGCTCCC TGTCTCAATC CCTGTCCCAG 
TCACTCCTCT CTGGAATGGC CTCCCAACAG AGCCAGACAC AGCCACCACC TGTTAAAACC 
GAGCATGGCC TTCTGGGAAC ACCCACATCT ACACAGAACT CCTTAGGTCT GAATGGCGGA 
GCAGGGGGTA TATGGGACTT TATGAGTGGC AGCTTCTCTC CCAGCCCGTC TCCAGTGTTC 
AGCAGCCTTA CTTCCAACAC GGTCGGCTCC AGCAGCGCAG ACATCGGCCG ACTCCTCCGA 
GAACTGGATG AAGCCAAGCG TAAGATCAAG CAGTGGGAGG AGGCCTGGCA CCAAGTCAAA 
CAGGCCTGTG AAGCATGGCA GAAAGACGCT CACGAAGCGA AAGAACAAGC AAAATCGGCA 
GAAGCAGAGC GTCAGCTGGC GGAGCAGAAG CGGGAGGACG CGGAGCGCAA GCTGAAGGAA 
CTGCAGGGAG ACTTTGACGC TTTGTGTCGT TCGCCCAACA CACCTCTTCT GCGCAGCTAC 
GGCGAGCTGG AGCAGCTCCC CCTGCCAAAG CTCCGCTCCA TCCAGAGCCA GCTACGCTCT 
GACCTAGACC TCATAGAAGG GGTAATATAT CAGCTTCAGT CAAAGAAATG TATAGTTTGC 
CAAAAGCATG ATCGTTCCAT TGTCCTGCAG CCTTGCCAAC ATTATGTACT TTGTCAGAAC 
TGTGCGTCTG GTAAAAAGGA ATGTCCTTAC TGTAAGACGA AAATATCCAA GTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164288.1
CDS	70..2526
Translation	MPSVSKTAASASPQTEKPTHYTYLKEFRTEQCPLFLQHKCTQHRPFTCFHWHFLNQRRRRPIRRRDGTFNYSPDVYCTKYDETTGICPDGDDCPYLHRTTGDTERKYHLRYYKTGTCIHETDARGHCVKNGLHCAFAHGPHDLRPPVYDIREIQAQEALQNGQLGSGEGIPDLQPGVLASQAMIEKTLNEDPRWQDTNFVLANYKTDQCTKPPRLCRQGYACPHYHNSRDRRRNPRKFKYRSTPCPSVKHGDEWGEPSKCESGDSCQYCHSRTEQQFHPEIYKSTKCNDMRQTGYCPRGPFCAFAHVERIPSTEETMSTLLSAMQSGSQSKLGSQYPECPVSEWSNNTNSITSSASNNGQSGNVSCSNSPTVTPSSGSNSSLSPIGPIGRPKCFTNGSLCSESTTSSVSSPTSNYPKAPGFEREDQAKNLKSQSGENNQKLMDQDKQAHGSVFSVVNPLASSITSSITSSLASSIGSDSSSPTTLSTMNAKATPFYPGSNTVESVIGSALDLNFSDINVASLDKELEDQDNNGLALTSQRLLGGSAPVNIPGSLARSSSLHSSSSLSASPLSSLSQSLSQSLLSGMASQQSQTQPPPVKTEHGLLGTPTSTQNSLGLNGGAGGIWDFMSGSFSPSPSPVFSSLTSNTVGSSSADIGRLLRELDEAKRKIKQWEEAWHQVKQACEAWQKDAHEAKEQAKSAEAERQLAEQKREDAERKLKELQGDFDALCRSPNTPLLRSYGELEQLPLPKLRSIQSQLRSDLDLIEGVIYQLQSKKCIVCQKHDRSIVLQPCQHYVLCQNCASGKKECPYCKTKISKW

Target ORF information:

RefSeq Version	XM_005164231.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio unkempt family zinc finger-like (unkl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164231.3

ORF Insert Sequence:

ATGCCGTCGG TTTCGAAAAC GGCGGCGAGC GCTTCTCCTC AAACCGAGAA ACCAACCCAC 
TATACATATT TGAAGGAGTT CAGGACCGAG CAGTGCCCAC TGTTTTTGCA GCACAAATGC 
ACACAGCATA GACCCTTTAC ATGTTTCCAC TGGCATTTTC TAAACCAGCG AAGACGGAGA 
CCCATACGGA GAAGAGACGG GACTTTTAAT TACAGCCCAG ATGTGTATTG CACGAAGTAT 
GATGAAACAA CAGGGATATG TCCCGACGGA GACGATTGCC CTTATTTACA CCGGACCACC 
GGTGACACGG AGCGAAAGTA TCATTTACGC TACTACAAAA CCGGCACCTG CATCCATGAG 
ACAGATGCCC GTGGACATTG TGTGAAGAAT GGCCTCCACT GCGCTTTCGC TCATGGACCA 
CATGACCTAC GGCCACCAGT TTATGACATC AGAGAGATCC AGGCACAAGA AGCTCTGCAG 
AATGGCCAGT TGGGATCTGG GGAGGGAATT CCTGACCTGC AGCCGGGTGT GCTGGCCAGC 
CAGGCTATGA TTGAGAAGAC CCTGAATGAG GACCCGCGCT GGCAAGACAC CAATTTCGTT 
TTGGCCAACT ATAAGACCGA TCAGTGTACC AAGCCTCCAC GTCTATGCAG GCAGGGCTAT 
GCTTGTCCCC ACTATCACAA CAGCAGAGAT AGAAGAAGAA ATCCCAGAAA GTTCAAATAC 
AGATCGACGC CCTGCCCGAG TGTGAAGCAT GGAGATGAAT GGGGCGAACC CTCCAAATGT 
GAGAGTGGAG ACAGCTGCCA GTACTGCCAC TCTCGCACCG AACAGCAGTT CCACCCAGAG 
ATTTACAAGT CAACTAAATG CAATGACATG AGGCAAACCG GATACTGTCC AAGGGGCCCG 
TTCTGTGCTT TTGCACATGT GGAAAGAATT CCTTCCACAG AAGAGACCAT GAGCACGTTG 
CTCTCAGCAA TGCAGTCTGG TTCTCAGTCT AAGCTGGGCT CTCAGTACCC AGAGTGTCCA 
GTCAGTGAGT GGAGCAACAA CACCAATTCC ATCACCAGCA GCGCTAGCAA CAACGGCCAG 
AGCGGAAACG TATCATGCTC TAATAGTCCA ACTGTAACAC CAAGCTCAGG GAGCAACAGT 
TCTTTGTCCC CAATTGGACC CATCGGCAGG CCAAAATGCT TTACTAATGG TAGCTTATGT 
TCAGAGTCCA CAACATCCAG TGTTTCCTCT CCAACGTCTA ACTATCCCAA AGCGCCGGGT 
TTTGAACGGG AGGATCAGGC AAAGAACCTG AAGAGCCAGT CTGGGGAGAA TAATCAGAAG 
TTAATGGACC AAGACAAACA GGCTCATGGC AGCGTGTTCT CCGTGGTGAA CCCACTAGCG 
TCCAGCATAA CATCAAGCAT AACGTCCAGC TTGGCCTCCA GCATCGGATC TGACAGTTCA 
TCGCCCACCA CTTTATCAAC CATGAATGCA AAAGCCACGC CTTTCTATCC GGGCAGCAAT 
ACTGTGGAGT CAGTAATAGG ATCTGCACTT GACCTAAATT TTAGTGACAT CAATGTTGCC 
TCTCTCGACA AAGAGCTAGA GGACCAAGAC AACAATGGCC TTGCTTTAAC AAGTCAGAGG 
TTATTAGGAG GCTCTGCTCC AGTTAATATT CCTGGCTCTC TGGCTCGGTC TTCCTCTCTG 
CACTCGTCAT CGTCTCTGTC TGCATCTCCG CTCAGCTCCC TGTCTCAATC CCTGTCCCAG 
TCACTCCTCT CTGGAATGGC CTCCCAACAG AGCCAGACAC AGCCACCACC TGTTAAAACC 
GAGCATGGCC TTCTGGGAAC ACCCACATCT ACACAGAACT CCTTAGGTCT GAATGGCGGA 
GCAGGGGGTA TATGGGACTT TATGAGTGGC AGCTTCTCTC CCAGCCCGTC TCCAGTGTTC 
AGCAGCCTTA CTTCCAACAC GGTCGGCTCC AGCAGCGCAG ACATCGGCCG ACTCCTCCGA 
GAACTGGATG AAGCCAAGCG TAAGATCAAG CAGTGGGAGG AGGCCTGGCA CCAAGTCAAA 
CAGGCCTGTG AAGCATGGCA GAAAGACGCT CACGAAGCGA AAGAACAAGC AAAATCGGCA 
GAAGCAGAGC GTCAGCTGGC GGAGCAGAAG CGGGAGGACG CGGAGCGCAA GCTGAAGGAA 
CTGCAGGGAG ACTTTGACGC TTTGTGTCGT TCGCCCAACA CACCTCTTCT GCGCAGCTAC 
GGCGAGCTGG AGCAGCTCCC CCTGCCAAAG CTCCGCTCCA TCCAGAGCCA GCTACGCTCT 
GACCTAGACC TCATAGAAGG GGTAATATAT CAGCTTCAGT CAAAGAAATG TATAGTTTGC 
CAAAAGCATG ATCGTTCCAT TGTCCTGCAG CCTTGCCAAC ATTATGTACT TTGTCAGAAC 
TGTGCGTCTG GTAAAAAGGA ATGTCCTTAC TGTAAGACGA AAATATCCAA GTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNKL ( XM_005164232.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005164289.1 Danio rerio unkempt family zinc finger-like (unkl), transcript variant X2, mRNA.

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CloneID	ODa08717
Clone ID Related Accession (Same CDS sequence)	XM_005164232.4 , XM_005164232.3
Accession Version	XM_005164232.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	putative E3 ubiquitin-protein ligase UNKL isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164232.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTCGG TTTCGAAAAC GGCGGCGAGC GCTTCTCCTC AAACCGAGAA ACCAACCCAC 
TATACATATT TGAAGGAGTT CAGGACCGAG CAGTGCCCAC TGTTTTTGCA GCACAAATGC 
ACACAGCATA GACCCTTTAC ATGTTTCCAC TGGCATTTTC TAAACCAGCG AAGACGGAGA 
CCCATACGGA GAAGAGACGG GACTTTTAAT TACAGCCCAG ATGTGTATTG CACGAAGTAT 
GATGAAACAA CAGGGATATG TCCCGACGGA GACGATTGCC CTTATTTACA CCGGACCACC 
GGTGACACGG AGCGAAAGTA TCATTTACGC TACTACAAAA CCGGCACCTG CATCCATGAG 
ACAGATGCCC GTGGACATTG TGTGAAGAAT GGCCTCCACT GCGCTTTCGC TCATGGACCA 
CATGACCTAC GGCCACCAGT TTATGACATC AGAGAGATCC AGGCACAAGA AGCTCTGCAG 
AATGGCCAGT TGGGATCTGG GGAGGGAATT CCTGACCTGC AGCCGGGTGT GCTGGCCAGC 
CAGGCTATGA TTGAGAAGAC CCTGAATGAG GACCCGCGCT GGCAAGACAC CAATTTCGTT 
TTGGCCAACT ATAAGACCGA TCAGTGTACC AAGCCTCCAC GTCTATGCAG GCAGGGCTAT 
GCTTGTCCCC ACTATCACAA CAGCAGAGAT AGAAGAAGAA ATCCCAGAAA GTTCAAATAC 
AGATCGACGC CCTGCCCGAG TGTGAAGCAT GGAGATGAAT GGGGCGAACC CTCCAAATGT 
GAGAGTGGAG ACAGCTGCCA GTACTGCCAC TCTCGCACCG AACAGCAGTT CCACCCAGAG 
ATTTACAAGT CAACTAAATG CAATGACATG AGGCAAACCG GATACTGTCC AAGGGGCCCG 
TTCTGTGCTT TTGCACATGT GGAAAGAATT CCTTCCACAG AAGAGACCAT GAGCACGTTG 
CTCTCAGCAA TGCAGTCTGG TTCTCAGTCT AAGCTGGGCT CTCAGTACCC AGAGTGTCCA 
GTCAGTGAGT GGAGCAACAA CACCAATTCC ATCACCAGCA GCGCTAGCAA CAACGGCCAG 
AGCGGAAACG CAAAGAACCT GAAGAGCCAG TCTGGGGAGA ATAATCAGAA GTTAATGGAC 
CAAGACAAAC AGGCTCATGG CAGCGTGTTC TCCGTGGTGA ACCCACTAGC GTCCAGCATA 
ACATCAAGCA TAACGTCCAG CTTGGCCTCC AGCATCGGAT CTGACAGTTC ATCGCCCACC 
ACTTTATCAA CCATGAATGC AAAAGCCACG CCTTTCTATC CGGGCAGCAA TACTGTGGAG 
TCAGTAATAG GATCTGCACT TGACCTAAAT TTTAGTGACA TCAATGTTGC CTCTCTCGAC 
AAAGAGCTAG AGGACCAAGA CAACAATGGC CTTGCTTTAA CAAGTCAGAG GTTATTAGGA 
GGCTCTGCTC CAGTTAATAT TCCTGGCTCT CTGGCTCGGT CTTCCTCTCT GCACTCGTCA 
TCGTCTCTGT CTGCATCTCC GCTCAGCTCC CTGTCTCAAT CCCTGTCCCA GTCACTCCTC 
TCTGGAATGG CCTCCCAACA GAGCCAGACA CAGCCACCAC CTGTTAAAAC CGAGCATGGC 
CTTCTGGGAA CACCCACATC TACACAGAAC TCCTTAGGTC TGAATGGCGG AGCAGGGGGT 
ATATGGGACT TTATGAGTGG CAGCTTCTCT CCCAGCCCGT CTCCAGTGTT CAGCAGCCTT 
ACTTCCAACA CGGTCGGCTC CAGCAGCGCA GACATCGGCC GACTCCTCCG AGAACTGGAT 
GAAGCCAAGC GTAAGATCAA GCAGTGGGAG GAGGCCTGGC ACCAAGTCAA ACAGGCCTGT 
GAAGCATGGC AGAAAGACGC TCACGAAGCG AAAGAACAAG CAAAATCGGC AGAAGCAGAG 
CGTCAGCTGG CGGAGCAGAA GCGGGAGGAC GCGGAGCGCA AGCTGAAGGA ACTGCAGGGA 
GACTTTGACG CTTTGTGTCG TTCGCCCAAC ACACCTCTTC TGCGCAGCTA CGGCGAGCTG 
GAGCAGCTCC CCCTGCCAAA GCTCCGCTCC ATCCAGAGCC AGCTACGCTC TGACCTAGAC 
CTCATAGAAG GGGTAATATA TCAGCTTCAG TCAAAGAAAT GTATAGTTTG CCAAAAGCAT 
GATCGTTCCA TTGTCCTGCA GCCTTGCCAA CATTATGTAC TTTGTCAGAA CTGTGCGTCT 
GGTAAAAAGG AATGTCCTTA CTGTAAGACG AAAATATCCA AGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164289.1
CDS	70..2337
Translation	MPSVSKTAASASPQTEKPTHYTYLKEFRTEQCPLFLQHKCTQHRPFTCFHWHFLNQRRRRPIRRRDGTFNYSPDVYCTKYDETTGICPDGDDCPYLHRTTGDTERKYHLRYYKTGTCIHETDARGHCVKNGLHCAFAHGPHDLRPPVYDIREIQAQEALQNGQLGSGEGIPDLQPGVLASQAMIEKTLNEDPRWQDTNFVLANYKTDQCTKPPRLCRQGYACPHYHNSRDRRRNPRKFKYRSTPCPSVKHGDEWGEPSKCESGDSCQYCHSRTEQQFHPEIYKSTKCNDMRQTGYCPRGPFCAFAHVERIPSTEETMSTLLSAMQSGSQSKLGSQYPECPVSEWSNNTNSITSSASNNGQSGNAKNLKSQSGENNQKLMDQDKQAHGSVFSVVNPLASSITSSITSSLASSIGSDSSSPTTLSTMNAKATPFYPGSNTVESVIGSALDLNFSDINVASLDKELEDQDNNGLALTSQRLLGGSAPVNIPGSLARSSSLHSSSSLSASPLSSLSQSLSQSLLSGMASQQSQTQPPPVKTEHGLLGTPTSTQNSLGLNGGAGGIWDFMSGSFSPSPSPVFSSLTSNTVGSSSADIGRLLRELDEAKRKIKQWEEAWHQVKQACEAWQKDAHEAKEQAKSAEAERQLAEQKREDAERKLKELQGDFDALCRSPNTPLLRSYGELEQLPLPKLRSIQSQLRSDLDLIEGVIYQLQSKKCIVCQKHDRSIVLQPCQHYVLCQNCASGKKECPYCKTKISKW

Target ORF information:

RefSeq Version	XM_005164232.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio unkempt family zinc finger-like (unkl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164232.4

ORF Insert Sequence:

ATGCCGTCGG TTTCGAAAAC GGCGGCGAGC GCTTCTCCTC AAACCGAGAA ACCAACCCAC 
TATACATATT TGAAGGAGTT CAGGACCGAG CAGTGCCCAC TGTTTTTGCA GCACAAATGC 
ACACAGCATA GACCCTTTAC ATGTTTCCAC TGGCATTTTC TAAACCAGCG AAGACGGAGA 
CCCATACGGA GAAGAGACGG GACTTTTAAT TACAGCCCAG ATGTGTATTG CACGAAGTAT 
GATGAAACAA CAGGGATATG TCCCGACGGA GACGATTGCC CTTATTTACA CCGGACCACC 
GGTGACACGG AGCGAAAGTA TCATTTACGC TACTACAAAA CCGGCACCTG CATCCATGAG 
ACAGATGCCC GTGGACATTG TGTGAAGAAT GGCCTCCACT GCGCTTTCGC TCATGGACCA 
CATGACCTAC GGCCACCAGT TTATGACATC AGAGAGATCC AGGCACAAGA AGCTCTGCAG 
AATGGCCAGT TGGGATCTGG GGAGGGAATT CCTGACCTGC AGCCGGGTGT GCTGGCCAGC 
CAGGCTATGA TTGAGAAGAC CCTGAATGAG GACCCGCGCT GGCAAGACAC CAATTTCGTT 
TTGGCCAACT ATAAGACCGA TCAGTGTACC AAGCCTCCAC GTCTATGCAG GCAGGGCTAT 
GCTTGTCCCC ACTATCACAA CAGCAGAGAT AGAAGAAGAA ATCCCAGAAA GTTCAAATAC 
AGATCGACGC CCTGCCCGAG TGTGAAGCAT GGAGATGAAT GGGGCGAACC CTCCAAATGT 
GAGAGTGGAG ACAGCTGCCA GTACTGCCAC TCTCGCACCG AACAGCAGTT CCACCCAGAG 
ATTTACAAGT CAACTAAATG CAATGACATG AGGCAAACCG GATACTGTCC AAGGGGCCCG 
TTCTGTGCTT TTGCACATGT GGAAAGAATT CCTTCCACAG AAGAGACCAT GAGCACGTTG 
CTCTCAGCAA TGCAGTCTGG TTCTCAGTCT AAGCTGGGCT CTCAGTACCC AGAGTGTCCA 
GTCAGTGAGT GGAGCAACAA CACCAATTCC ATCACCAGCA GCGCTAGCAA CAACGGCCAG 
AGCGGAAACG CAAAGAACCT GAAGAGCCAG TCTGGGGAGA ATAATCAGAA GTTAATGGAC 
CAAGACAAAC AGGCTCATGG CAGCGTGTTC TCCGTGGTGA ACCCACTAGC GTCCAGCATA 
ACATCAAGCA TAACGTCCAG CTTGGCCTCC AGCATCGGAT CTGACAGTTC ATCGCCCACC 
ACTTTATCAA CCATGAATGC AAAAGCCACG CCTTTCTATC CGGGCAGCAA TACTGTGGAG 
TCAGTAATAG GATCTGCACT TGACCTAAAT TTTAGTGACA TCAATGTTGC CTCTCTCGAC 
AAAGAGCTAG AGGACCAAGA CAACAATGGC CTTGCTTTAA CAAGTCAGAG GTTATTAGGA 
GGCTCTGCTC CAGTTAATAT TCCTGGCTCT CTGGCTCGGT CTTCCTCTCT GCACTCGTCA 
TCGTCTCTGT CTGCATCTCC GCTCAGCTCC CTGTCTCAAT CCCTGTCCCA GTCACTCCTC 
TCTGGAATGG CCTCCCAACA GAGCCAGACA CAGCCACCAC CTGTTAAAAC CGAGCATGGC 
CTTCTGGGAA CACCCACATC TACACAGAAC TCCTTAGGTC TGAATGGCGG AGCAGGGGGT 
ATATGGGACT TTATGAGTGG CAGCTTCTCT CCCAGCCCGT CTCCAGTGTT CAGCAGCCTT 
ACTTCCAACA CGGTCGGCTC CAGCAGCGCA GACATCGGCC GACTCCTCCG AGAACTGGAT 
GAAGCCAAGC GTAAGATCAA GCAGTGGGAG GAGGCCTGGC ACCAAGTCAA ACAGGCCTGT 
GAAGCATGGC AGAAAGACGC TCACGAAGCG AAAGAACAAG CAAAATCGGC AGAAGCAGAG 
CGTCAGCTGG CGGAGCAGAA GCGGGAGGAC GCGGAGCGCA AGCTGAAGGA ACTGCAGGGA 
GACTTTGACG CTTTGTGTCG TTCGCCCAAC ACACCTCTTC TGCGCAGCTA CGGCGAGCTG 
GAGCAGCTCC CCCTGCCAAA GCTCCGCTCC ATCCAGAGCC AGCTACGCTC TGACCTAGAC 
CTCATAGAAG GGGTAATATA TCAGCTTCAG TCAAAGAAAT GTATAGTTTG CCAAAAGCAT 
GATCGTTCCA TTGTCCTGCA GCCTTGCCAA CATTATGTAC TTTGTCAGAA CTGTGCGTCT 
GGTAAAAAGG AATGTCCTTA CTGTAAGACG AAAATATCCA AGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

UNKL ( XM_005164232.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005164289.1 Danio rerio unkempt family zinc finger-like (unkl), transcript variant X2, mRNA.

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CloneID	ODa08717
Clone ID Related Accession (Same CDS sequence)	XM_005164232.4 , XM_005164232.3
Accession Version	XM_005164232.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	putative E3 ubiquitin-protein ligase UNKL isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164232.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGTCGG TTTCGAAAAC GGCGGCGAGC GCTTCTCCTC AAACCGAGAA ACCAACCCAC 
TATACATATT TGAAGGAGTT CAGGACCGAG CAGTGCCCAC TGTTTTTGCA GCACAAATGC 
ACACAGCATA GACCCTTTAC ATGTTTCCAC TGGCATTTTC TAAACCAGCG AAGACGGAGA 
CCCATACGGA GAAGAGACGG GACTTTTAAT TACAGCCCAG ATGTGTATTG CACGAAGTAT 
GATGAAACAA CAGGGATATG TCCCGACGGA GACGATTGCC CTTATTTACA CCGGACCACC 
GGTGACACGG AGCGAAAGTA TCATTTACGC TACTACAAAA CCGGCACCTG CATCCATGAG 
ACAGATGCCC GTGGACATTG TGTGAAGAAT GGCCTCCACT GCGCTTTCGC TCATGGACCA 
CATGACCTAC GGCCACCAGT TTATGACATC AGAGAGATCC AGGCACAAGA AGCTCTGCAG 
AATGGCCAGT TGGGATCTGG GGAGGGAATT CCTGACCTGC AGCCGGGTGT GCTGGCCAGC 
CAGGCTATGA TTGAGAAGAC CCTGAATGAG GACCCGCGCT GGCAAGACAC CAATTTCGTT 
TTGGCCAACT ATAAGACCGA TCAGTGTACC AAGCCTCCAC GTCTATGCAG GCAGGGCTAT 
GCTTGTCCCC ACTATCACAA CAGCAGAGAT AGAAGAAGAA ATCCCAGAAA GTTCAAATAC 
AGATCGACGC CCTGCCCGAG TGTGAAGCAT GGAGATGAAT GGGGCGAACC CTCCAAATGT 
GAGAGTGGAG ACAGCTGCCA GTACTGCCAC TCTCGCACCG AACAGCAGTT CCACCCAGAG 
ATTTACAAGT CAACTAAATG CAATGACATG AGGCAAACCG GATACTGTCC AAGGGGCCCG 
TTCTGTGCTT TTGCACATGT GGAAAGAATT CCTTCCACAG AAGAGACCAT GAGCACGTTG 
CTCTCAGCAA TGCAGTCTGG TTCTCAGTCT AAGCTGGGCT CTCAGTACCC AGAGTGTCCA 
GTCAGTGAGT GGAGCAACAA CACCAATTCC ATCACCAGCA GCGCTAGCAA CAACGGCCAG 
AGCGGAAACG CAAAGAACCT GAAGAGCCAG TCTGGGGAGA ATAATCAGAA GTTAATGGAC 
CAAGACAAAC AGGCTCATGG CAGCGTGTTC TCCGTGGTGA ACCCACTAGC GTCCAGCATA 
ACATCAAGCA TAACGTCCAG CTTGGCCTCC AGCATCGGAT CTGACAGTTC ATCGCCCACC 
ACTTTATCAA CCATGAATGC AAAAGCCACG CCTTTCTATC CGGGCAGCAA TACTGTGGAG 
TCAGTAATAG GATCTGCACT TGACCTAAAT TTTAGTGACA TCAATGTTGC CTCTCTCGAC 
AAAGAGCTAG AGGACCAAGA CAACAATGGC CTTGCTTTAA CAAGTCAGAG GTTATTAGGA 
GGCTCTGCTC CAGTTAATAT TCCTGGCTCT CTGGCTCGGT CTTCCTCTCT GCACTCGTCA 
TCGTCTCTGT CTGCATCTCC GCTCAGCTCC CTGTCTCAAT CCCTGTCCCA GTCACTCCTC 
TCTGGAATGG CCTCCCAACA GAGCCAGACA CAGCCACCAC CTGTTAAAAC CGAGCATGGC 
CTTCTGGGAA CACCCACATC TACACAGAAC TCCTTAGGTC TGAATGGCGG AGCAGGGGGT 
ATATGGGACT TTATGAGTGG CAGCTTCTCT CCCAGCCCGT CTCCAGTGTT CAGCAGCCTT 
ACTTCCAACA CGGTCGGCTC CAGCAGCGCA GACATCGGCC GACTCCTCCG AGAACTGGAT 
GAAGCCAAGC GTAAGATCAA GCAGTGGGAG GAGGCCTGGC ACCAAGTCAA ACAGGCCTGT 
GAAGCATGGC AGAAAGACGC TCACGAAGCG AAAGAACAAG CAAAATCGGC AGAAGCAGAG 
CGTCAGCTGG CGGAGCAGAA GCGGGAGGAC GCGGAGCGCA AGCTGAAGGA ACTGCAGGGA 
GACTTTGACG CTTTGTGTCG TTCGCCCAAC ACACCTCTTC TGCGCAGCTA CGGCGAGCTG 
GAGCAGCTCC CCCTGCCAAA GCTCCGCTCC ATCCAGAGCC AGCTACGCTC TGACCTAGAC 
CTCATAGAAG GGGTAATATA TCAGCTTCAG TCAAAGAAAT GTATAGTTTG CCAAAAGCAT 
GATCGTTCCA TTGTCCTGCA GCCTTGCCAA CATTATGTAC TTTGTCAGAA CTGTGCGTCT 
GGTAAAAAGG AATGTCCTTA CTGTAAGACG AAAATATCCA AGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164289.1
CDS	69..2336
Translation	MPSVSKTAASASPQTEKPTHYTYLKEFRTEQCPLFLQHKCTQHRPFTCFHWHFLNQRRRRPIRRRDGTFNYSPDVYCTKYDETTGICPDGDDCPYLHRTTGDTERKYHLRYYKTGTCIHETDARGHCVKNGLHCAFAHGPHDLRPPVYDIREIQAQEALQNGQLGSGEGIPDLQPGVLASQAMIEKTLNEDPRWQDTNFVLANYKTDQCTKPPRLCRQGYACPHYHNSRDRRRNPRKFKYRSTPCPSVKHGDEWGEPSKCESGDSCQYCHSRTEQQFHPEIYKSTKCNDMRQTGYCPRGPFCAFAHVERIPSTEETMSTLLSAMQSGSQSKLGSQYPECPVSEWSNNTNSITSSASNNGQSGNAKNLKSQSGENNQKLMDQDKQAHGSVFSVVNPLASSITSSITSSLASSIGSDSSSPTTLSTMNAKATPFYPGSNTVESVIGSALDLNFSDINVASLDKELEDQDNNGLALTSQRLLGGSAPVNIPGSLARSSSLHSSSSLSASPLSSLSQSLSQSLLSGMASQQSQTQPPPVKTEHGLLGTPTSTQNSLGLNGGAGGIWDFMSGSFSPSPSPVFSSLTSNTVGSSSADIGRLLRELDEAKRKIKQWEEAWHQVKQACEAWQKDAHEAKEQAKSAEAERQLAEQKREDAERKLKELQGDFDALCRSPNTPLLRSYGELEQLPLPKLRSIQSQLRSDLDLIEGVIYQLQSKKCIVCQKHDRSIVLQPCQHYVLCQNCASGKKECPYCKTKISKW

Target ORF information:

RefSeq Version	XM_005164232.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio unkempt family zinc finger-like (unkl), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164232.3

ORF Insert Sequence:

ATGCCGTCGG TTTCGAAAAC GGCGGCGAGC GCTTCTCCTC AAACCGAGAA ACCAACCCAC 
TATACATATT TGAAGGAGTT CAGGACCGAG CAGTGCCCAC TGTTTTTGCA GCACAAATGC 
ACACAGCATA GACCCTTTAC ATGTTTCCAC TGGCATTTTC TAAACCAGCG AAGACGGAGA 
CCCATACGGA GAAGAGACGG GACTTTTAAT TACAGCCCAG ATGTGTATTG CACGAAGTAT 
GATGAAACAA CAGGGATATG TCCCGACGGA GACGATTGCC CTTATTTACA CCGGACCACC 
GGTGACACGG AGCGAAAGTA TCATTTACGC TACTACAAAA CCGGCACCTG CATCCATGAG 
ACAGATGCCC GTGGACATTG TGTGAAGAAT GGCCTCCACT GCGCTTTCGC TCATGGACCA 
CATGACCTAC GGCCACCAGT TTATGACATC AGAGAGATCC AGGCACAAGA AGCTCTGCAG 
AATGGCCAGT TGGGATCTGG GGAGGGAATT CCTGACCTGC AGCCGGGTGT GCTGGCCAGC 
CAGGCTATGA TTGAGAAGAC CCTGAATGAG GACCCGCGCT GGCAAGACAC CAATTTCGTT 
TTGGCCAACT ATAAGACCGA TCAGTGTACC AAGCCTCCAC GTCTATGCAG GCAGGGCTAT 
GCTTGTCCCC ACTATCACAA CAGCAGAGAT AGAAGAAGAA ATCCCAGAAA GTTCAAATAC 
AGATCGACGC CCTGCCCGAG TGTGAAGCAT GGAGATGAAT GGGGCGAACC CTCCAAATGT 
GAGAGTGGAG ACAGCTGCCA GTACTGCCAC TCTCGCACCG AACAGCAGTT CCACCCAGAG 
ATTTACAAGT CAACTAAATG CAATGACATG AGGCAAACCG GATACTGTCC AAGGGGCCCG 
TTCTGTGCTT TTGCACATGT GGAAAGAATT CCTTCCACAG AAGAGACCAT GAGCACGTTG 
CTCTCAGCAA TGCAGTCTGG TTCTCAGTCT AAGCTGGGCT CTCAGTACCC AGAGTGTCCA 
GTCAGTGAGT GGAGCAACAA CACCAATTCC ATCACCAGCA GCGCTAGCAA CAACGGCCAG 
AGCGGAAACG CAAAGAACCT GAAGAGCCAG TCTGGGGAGA ATAATCAGAA GTTAATGGAC 
CAAGACAAAC AGGCTCATGG CAGCGTGTTC TCCGTGGTGA ACCCACTAGC GTCCAGCATA 
ACATCAAGCA TAACGTCCAG CTTGGCCTCC AGCATCGGAT CTGACAGTTC ATCGCCCACC 
ACTTTATCAA CCATGAATGC AAAAGCCACG CCTTTCTATC CGGGCAGCAA TACTGTGGAG 
TCAGTAATAG GATCTGCACT TGACCTAAAT TTTAGTGACA TCAATGTTGC CTCTCTCGAC 
AAAGAGCTAG AGGACCAAGA CAACAATGGC CTTGCTTTAA CAAGTCAGAG GTTATTAGGA 
GGCTCTGCTC CAGTTAATAT TCCTGGCTCT CTGGCTCGGT CTTCCTCTCT GCACTCGTCA 
TCGTCTCTGT CTGCATCTCC GCTCAGCTCC CTGTCTCAAT CCCTGTCCCA GTCACTCCTC 
TCTGGAATGG CCTCCCAACA GAGCCAGACA CAGCCACCAC CTGTTAAAAC CGAGCATGGC 
CTTCTGGGAA CACCCACATC TACACAGAAC TCCTTAGGTC TGAATGGCGG AGCAGGGGGT 
ATATGGGACT TTATGAGTGG CAGCTTCTCT CCCAGCCCGT CTCCAGTGTT CAGCAGCCTT 
ACTTCCAACA CGGTCGGCTC CAGCAGCGCA GACATCGGCC GACTCCTCCG AGAACTGGAT 
GAAGCCAAGC GTAAGATCAA GCAGTGGGAG GAGGCCTGGC ACCAAGTCAA ACAGGCCTGT 
GAAGCATGGC AGAAAGACGC TCACGAAGCG AAAGAACAAG CAAAATCGGC AGAAGCAGAG 
CGTCAGCTGG CGGAGCAGAA GCGGGAGGAC GCGGAGCGCA AGCTGAAGGA ACTGCAGGGA 
GACTTTGACG CTTTGTGTCG TTCGCCCAAC ACACCTCTTC TGCGCAGCTA CGGCGAGCTG 
GAGCAGCTCC CCCTGCCAAA GCTCCGCTCC ATCCAGAGCC AGCTACGCTC TGACCTAGAC 
CTCATAGAAG GGGTAATATA TCAGCTTCAG TCAAAGAAAT GTATAGTTTG CCAAAAGCAT 
GATCGTTCCA TTGTCCTGCA GCCTTGCCAA CATTATGTAC TTTGTCAGAA CTGTGCGTCT 
GGTAAAAAGG AATGTCCTTA CTGTAAGACG AAAATATCCA AGTGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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UNKL cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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