EMC1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	EMC1
Entrez Gene ID	565399
Full Name	ER membrane protein complex subunit 1
Synonyms	im:7158249,si:dkey-7c22.1,wu:fc76f12
General protein information	Preferred Names ER membrane protein complex subunit 1 Names ER membrane protein complex subunit 1
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021473642.1	XP_021329317.1	ER membrane protein complex subunit 1 isoform X2
XM_005162189.4	XP_005162246.1	ER membrane protein complex subunit 1 isoform X1
XM_688678.9	XP_693770.3	ER membrane protein complex subunit 1 isoform X2
XM_688678.8	XP_693770.3	ER membrane protein complex subunit 1 isoform X2
XM_021473641.1	XP_021329316.1	ER membrane protein complex subunit 1 isoform X1
XM_005162189.3	XP_005162246.1	ER membrane protein complex subunit 1 isoform X1

Pathways from BioSystems

Homologies

	EMC1	XP_001091700.1
Canis lupus familiaris (dog)	EMC1	XP_005617944.1
Drosophila melanogaster (fruit fly)	CG2943	NP_649747.1
Mus musculus (house mouse)	Emc1	NP_666269.2
Arabidopsis thaliana (thale cress)	AT5G11560	NP_196717.3
Bos taurus (cattle)	EMC1	XP_005203411.1
Danio rerio (zebrafish)	emc1	XP_693770.3
Caenorhabditis elegans (roundworm)	H17B01.4	NP_493980.1
Oryza sativa (rice)	Os05g0230600	NP_001054975.1
Gallus gallus (chicken)	EMC1	NP_001012856.1
Homo sapiens (human)	EMC1	NP_055862.1
Pan troglodytes (chimpanzee)	EMC1	XP_001162981.2
Rattus norvegicus (Norway rat)	Emc1	NP_001102160.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003647	XP_562511.3
Xenopus tropicalis (tropical clawed frog)	emc1	XP_002943252.2

Gene Ontology

The following EMC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EMC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa27727	XM_005162189.4 Latest version!	Danio rerio ER membrane protein complex subunit 1 (emc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa27728	XM_688678.9 Latest version!	Danio rerio ER membrane protein complex subunit 1 (emc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa146258	XM_021473641.1 Latest version!	Danio rerio ER membrane protein complex subunit 1 (emc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa146259	XM_021473642.1 Latest version!	Danio rerio ER membrane protein complex subunit 1 (emc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
View Old Accession Versions >>
ODa27728	XM_688678.8	Danio rerio ER membrane protein complex subunit 1 (emc1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa27727	XM_005162189.3	Danio rerio ER membrane protein complex subunit 1 (emc1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EMC1 ( XM_005162189.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005162246.1 Danio rerio ER membrane protein complex subunit 1 (emc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27727
Clone ID Related Accession (Same CDS sequence)	XM_005162189.4 , XM_005162189.3
Accession Version	XM_005162189.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ER membrane protein complex subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005162189.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGGC TTGTAGTCAG ATTAGCAATA TCGGTGTCTT TATTATATAC TGCATCGGCG 
GTATTTGAAG ACCAGGTTGG AAAGTTTGAT TGGAGGCAAC AGTACATCGG CAAGGTGCGT 
TTTGCCTTGT TTGATACTCA TTCTCAAGCG TCTAAAAAGC TCCTGGTGGC CACCGACAAG 
AACGTGTTTG CTTCCCTAAA CTCAAGGACT GGAGACCTGT TCTGGCGTCA TGTGGACAAG 
ACTGGACCCG AGGGCCATAT TGATGCACTG CTTATGCATG GCCAGGATGC TGTTGTTGTT 
GTCGGGAATG GCCGTCTGCT TCGCTCCTGG GAGACTACAG TCGGAGGCTT GAAGTGGGAG 
ACTGTGCTTG ATTCTGGAAG TTTTCAGGCC GCTGCTTTAG TTGGAGTTCA AGATTATGTG 
AAGTACGTTG CTGTGCTGAA GAAATCGACA ATCGCTCTCC ATGACCTTTC AAGTGGCAGC 
CAAACATGGG TGGAAAACCT ACCTGACAGT GACACTGTTC AGTATCAAAC CATTTACTCT 
GGGGGGAATG GCCTAGTGTT TGTTTTGGGA GTTTTGCCAA ACTCTCACAT TGTTATTGTT 
GAATACAAAA TTGAAGATGG AGAAATCATG AACAAGAAAT CAGTTGAAGC CGCCTGGATG 
TCTAGCCTGG AGAGCAGCTG TGTAGTGGTC AGCTCAGGGA TCCTGATGTG TGTGGATCAG 
ATCACACAGT CCCTGTACAC TCAACCACTG CACTCTGCCG AACAAACAGA AATGAGACAG 
ATCCATCTTC AGACGCTAGA TTTGGAGGTG GCGTCTGGAT TCCAGCCTGT TTTGACATCC 
ACTCAGCCCA GTCCAGCACA TCCTCCGCTG GCTGAGTTCA TCCTGCAGCT CAGTCCAGAA 
CATCACATCT TGCTGCAGCT CAAAGACGGA CTTATCGCGC CGCTCCGAGA CTTCAGTCCA 
TCTTATCTTG CTGCTTTTGC CACATCTGGA GAGAAGACAG TTGTTGCTGT GATGTCCCCA 
AAGAATGATA CTGCCTGCAG CATCAATCTG TTCAGTGCTG ACACTGGCCG CAGGCACCTT 
GACACAACCA TTATCTACCA CACAGACCCT TACGGAGGAA AACCAAATAA ATTGTATGTC 
CACGCCTTCC TAAAAAAAGA TGACTCGGTG GGCTACAGAG TCATGGTACA GACGGAAGAC 
CTTACACTCA CATTTCTGCA GCAGCCTGGC AGGGTGGTCT GGATGAGGGA GGAGGCTCTG 
GCTGATGTGG TAAACATGGA GATGGTTGAT CTGCCACTTA CTGGGACACA GGCAGAGCTG 
GAAGGAGAGT TTGGGAAGAA AGCTGCCATT CAAGACGGAC TGTTACCCAT GGTTCTCAAG 
CGTCTCTCTT CCCAGTTCAT CTTGCTACAG GCCTGGATGG CTCATCTCTG GAAACTTTTC 
TATGATGCCC GAAAGCCCCG CAGCAGTGTG AAGAACGAGA TCACCATAGA CACACTGTCT 
CGTGACGAGT TCAACCTGCA GAAAATGATG GTAATGGTTA CTGCCTCTGG GAAGCTCTTT 
GGCATCGACA GTAGATCAGG GACTGTTTTG TGGAAGCAGT ATTTAGAAAA CATCAAGCCC 
AATTCGTTTT TCAAGCTCAT TGTGCAAAGG ACTACTGCCC ACTTTCCACA TCCACCACAG 
TGCACACTTC TTATAAAGGA CCAGGATACA GGGCTTGGAA GCCTGTATGT CTTCAATCCC 
ATCTTCGGCA AGAAAAGTCA AATTAGTGTC CCAGCGTTAC CCAGACCTAT CCTTCAGTCT 
CTTCTGTTAC CTGTCATCGA CCAGGACTAT TCTAAAGTCC TTCTACTTAT TGATGATCAG 
AACAAAGTCA CAGCATTTCC ATCTACTAAG AATATTCTGC AGCAGCTTCA GGAGACTGCA 
TCTTCCATAT TTTTCTACCT AGTGGACTCC AACCAGGGAA AGCTGTCGGG TTTTCGTCTG 
CGCACGGATT TGTCTACAGA ACTGATCTGG GAAGTTGTTA TCCCTACCGA GGTGCAGAAG 
ATAGTGGCTG TCAAAGGGAA GCGTGCAAAT GAACACGTGC ATTCCCAAGG CAGAGTCATG 
GGGGATCGTA GCGTGCTCTA CAAATATCTG AACCCTAATC TCTTGGCTGT GATAACTGAA 
AGCACAGACA CTCATCAGGA ACGCAGCTTT GTTGGAATCT TCCTGATTGA CGGTGTGACT 
GGCCGTATTG TGCATGAGGC TGTGCAGCGA AAGGCCAGAG GGCCAGTTCA CTGTGTGCAC 
TCTGAAAACT GGGTGATTTA TGTGTACTGG AACTCCAAGT TTCGCAGGAA TGAGTTTTCT 
GTGTTGGAGC TGTTTGAGGG AGCAGAGCTC TATAACAGCA CGGTGTTCAG CTCTCTGGAC 
AGACCTCATC CACCTCAGGT GCTGCAGCAG TCCTACATCT TTCCTGCTCC TATAAGCACT 
TTGGAGGCCA CACTTACAGA AAAAGGCATC ACCAGTCGAC ACCTTCTCGT GGGGCTGCCA 
TCAGGCAACA TTTTATCCTT ACCAAAGATG TTTTTGGACC CCAGGAGACC AGAGGTGGTC 
TCAGAACAGA GTCGGGAGGA AAATCTTATA CCATATGCTC CAGAAATGCC CATTCGTACA 
GAATGGTTCA TTAACTACAA CCAAACCGTA TCAAGAGTAA GAGGCATCTA TACTGCCCCA 
TCTGGCCTGG AGTCAACCTG TTTGGTGGTT GCATACGGCC TTGACATCTA CCAGACTAGA 
GTGTTCCCTT CCAAACAATT TGATGTCCTT AAGGATGACT ATGACTATGT CTTAATCAGC 
AGTGTCCTCC TCGGTCTCTT CTTTGCCACC ATGATCAGCA AACGTCTAGC GGAAGTGAAA 
CTGCTCAATA GGGCATGGCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162246.1
CDS	36..2939
Translation	MAWLVVRLAISVSLLYTASAVFEDQVGKFDWRQQYIGKVRFALFDTHSQASKKLLVATDKNVFASLNSRTGDLFWRHVDKTGPEGHIDALLMHGQDAVVVVGNGRLLRSWETTVGGLKWETVLDSGSFQAAALVGVQDYVKYVAVLKKSTIALHDLSSGSQTWVENLPDSDTVQYQTIYSGGNGLVFVLGVLPNSHIVIVEYKIEDGEIMNKKSVEAAWMSSLESSCVVVSSGILMCVDQITQSLYTQPLHSAEQTEMRQIHLQTLDLEVASGFQPVLTSTQPSPAHPPLAEFILQLSPEHHILLQLKDGLIAPLRDFSPSYLAAFATSGEKTVVAVMSPKNDTACSINLFSADTGRRHLDTTIIYHTDPYGGKPNKLYVHAFLKKDDSVGYRVMVQTEDLTLTFLQQPGRVVWMREEALADVVNMEMVDLPLTGTQAELEGEFGKKAAIQDGLLPMVLKRLSSQFILLQAWMAHLWKLFYDARKPRSSVKNEITIDTLSRDEFNLQKMMVMVTASGKLFGIDSRSGTVLWKQYLENIKPNSFFKLIVQRTTAHFPHPPQCTLLIKDQDTGLGSLYVFNPIFGKKSQISVPALPRPILQSLLLPVIDQDYSKVLLLIDDQNKVTAFPSTKNILQQLQETASSIFFYLVDSNQGKLSGFRLRTDLSTELIWEVVIPTEVQKIVAVKGKRANEHVHSQGRVMGDRSVLYKYLNPNLLAVITESTDTHQERSFVGIFLIDGVTGRIVHEAVQRKARGPVHCVHSENWVIYVYWNSKFRRNEFSVLELFEGAELYNSTVFSSLDRPHPPQVLQQSYIFPAPISTLEATLTEKGITSRHLLVGLPSGNILSLPKMFLDPRRPEVVSEQSREENLIPYAPEMPIRTEWFINYNQTVSRVRGIYTAPSGLESTCLVVAYGLDIYQTRVFPSKQFDVLKDDYDYVLISSVLLGLFFATMISKRLAEVKLLNRAWR

Target ORF information:

RefSeq Version	XM_005162189.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ER membrane protein complex subunit 1 (emc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162189.4

ORF Insert Sequence:

ATGGCTTGGC TTGTAGTCAG ATTAGCAATA TCGGTGTCTT TATTATATAC TGCATCGGCG 
GTATTTGAAG ACCAGGTTGG AAAGTTTGAT TGGAGGCAAC AGTACATCGG CAAGGTGCGT 
TTTGCCTTGT TTGATACTCA TTCTCAAGCG TCTAAAAAGC TCCTGGTGGC CACCGACAAG 
AACGTGTTTG CTTCCCTAAA CTCAAGGACT GGAGACCTGT TCTGGCGTCA TGTGGACAAG 
ACTGGACCCG AGGGCCATAT TGATGCACTG CTTATGCATG GCCAGGATGC TGTTGTTGTT 
GTCGGGAATG GCCGTCTGCT TCGCTCCTGG GAGACTACAG TCGGAGGCTT GAAGTGGGAG 
ACTGTGCTTG ATTCTGGAAG TTTTCAGGCC GCTGCTTTAG TTGGAGTTCA AGATTATGTG 
AAGTACGTTG CTGTGCTGAA GAAATCGACA ATCGCTCTCC ATGACCTTTC AAGTGGCAGC 
CAAACATGGG TGGAAAACCT ACCTGACAGT GACACTGTTC AGTATCAAAC CATTTACTCT 
GGGGGGAATG GCCTAGTGTT TGTTTTGGGA GTTTTGCCAA ACTCTCACAT TGTTATTGTT 
GAATACAAAA TTGAAGATGG AGAAATCATG AACAAGAAAT CAGTTGAAGC CGCCTGGATG 
TCTAGCCTGG AGAGCAGCTG TGTAGTGGTC AGCTCAGGGA TCCTGATGTG TGTGGATCAG 
ATCACACAGT CCCTGTACAC TCAACCACTG CACTCTGCCG AACAAACAGA AATGAGACAG 
ATCCATCTTC AGACGCTAGA TTTGGAGGTG GCGTCTGGAT TCCAGCCTGT TTTGACATCC 
ACTCAGCCCA GTCCAGCACA TCCTCCGCTG GCTGAGTTCA TCCTGCAGCT CAGTCCAGAA 
CATCACATCT TGCTGCAGCT CAAAGACGGA CTTATCGCGC CGCTCCGAGA CTTCAGTCCA 
TCTTATCTTG CTGCTTTTGC CACATCTGGA GAGAAGACAG TTGTTGCTGT GATGTCCCCA 
AAGAATGATA CTGCCTGCAG CATCAATCTG TTCAGTGCTG ACACTGGCCG CAGGCACCTT 
GACACAACCA TTATCTACCA CACAGACCCT TACGGAGGAA AACCAAATAA ATTGTATGTC 
CACGCCTTCC TAAAAAAAGA TGACTCGGTG GGCTACAGAG TCATGGTACA GACGGAAGAC 
CTTACACTCA CATTTCTGCA GCAGCCTGGC AGGGTGGTCT GGATGAGGGA GGAGGCTCTG 
GCTGATGTGG TAAACATGGA GATGGTTGAT CTGCCACTTA CTGGGACACA GGCAGAGCTG 
GAAGGAGAGT TTGGGAAGAA AGCTGCCATT CAAGACGGAC TGTTACCCAT GGTTCTCAAG 
CGTCTCTCTT CCCAGTTCAT CTTGCTACAG GCCTGGATGG CTCATCTCTG GAAACTTTTC 
TATGATGCCC GAAAGCCCCG CAGCAGTGTG AAGAACGAGA TCACCATAGA CACACTGTCT 
CGTGACGAGT TCAACCTGCA GAAAATGATG GTAATGGTTA CTGCCTCTGG GAAGCTCTTT 
GGCATCGACA GTAGATCAGG GACTGTTTTG TGGAAGCAGT ATTTAGAAAA CATCAAGCCC 
AATTCGTTTT TCAAGCTCAT TGTGCAAAGG ACTACTGCCC ACTTTCCACA TCCACCACAG 
TGCACACTTC TTATAAAGGA CCAGGATACA GGGCTTGGAA GCCTGTATGT CTTCAATCCC 
ATCTTCGGCA AGAAAAGTCA AATTAGTGTC CCAGCGTTAC CCAGACCTAT CCTTCAGTCT 
CTTCTGTTAC CTGTCATCGA CCAGGACTAT TCTAAAGTCC TTCTACTTAT TGATGATCAG 
AACAAAGTCA CAGCATTTCC ATCTACTAAG AATATTCTGC AGCAGCTTCA GGAGACTGCA 
TCTTCCATAT TTTTCTACCT AGTGGACTCC AACCAGGGAA AGCTGTCGGG TTTTCGTCTG 
CGCACGGATT TGTCTACAGA ACTGATCTGG GAAGTTGTTA TCCCTACCGA GGTGCAGAAG 
ATAGTGGCTG TCAAAGGGAA GCGTGCAAAT GAACACGTGC ATTCCCAAGG CAGAGTCATG 
GGGGATCGTA GCGTGCTCTA CAAATATCTG AACCCTAATC TCTTGGCTGT GATAACTGAA 
AGCACAGACA CTCATCAGGA ACGCAGCTTT GTTGGAATCT TCCTGATTGA CGGTGTGACT 
GGCCGTATTG TGCATGAGGC TGTGCAGCGA AAGGCCAGAG GGCCAGTTCA CTGTGTGCAC 
TCTGAAAACT GGGTGATTTA TGTGTACTGG AACTCCAAGT TTCGCAGGAA TGAGTTTTCT 
GTGTTGGAGC TGTTTGAGGG AGCAGAGCTC TATAACAGCA CGGTGTTCAG CTCTCTGGAC 
AGACCTCATC CACCTCAGGT GCTGCAGCAG TCCTACATCT TTCCTGCTCC TATAAGCACT 
TTGGAGGCCA CACTTACAGA AAAAGGCATC ACCAGTCGAC ACCTTCTCGT GGGGCTGCCA 
TCAGGCAACA TTTTATCCTT ACCAAAGATG TTTTTGGACC CCAGGAGACC AGAGGTGGTC 
TCAGAACAGA GTCGGGAGGA AAATCTTATA CCATATGCTC CAGAAATGCC CATTCGTACA 
GAATGGTTCA TTAACTACAA CCAAACCGTA TCAAGAGTAA GAGGCATCTA TACTGCCCCA 
TCTGGCCTGG AGTCAACCTG TTTGGTGGTT GCATACGGCC TTGACATCTA CCAGACTAGA 
GTGTTCCCTT CCAAACAATT TGATGTCCTT AAGGATGACT ATGACTATGT CTTAATCAGC 
AGTGTCCTCC TCGGTCTCTT CTTTGCCACC ATGATCAGCA AACGTCTAGC GGAAGTGAAA 
CTGCTCAATA GGGCATGGCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMC1 ( XM_005162189.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005162246.1 Danio rerio ER membrane protein complex subunit 1 (emc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa27727
Clone ID Related Accession (Same CDS sequence)	XM_005162189.4 , XM_005162189.3
Accession Version	XM_005162189.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ER membrane protein complex subunit 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005162189.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGGC TTGTAGTCAG ATTAGCAATA TCGGTGTCTT TATTATATAC TGCATCGGCG 
GTATTTGAAG ACCAGGTTGG AAAGTTTGAT TGGAGGCAAC AGTACATCGG CAAGGTGCGT 
TTTGCCTTGT TTGATACTCA TTCTCAAGCG TCTAAAAAGC TCCTGGTGGC CACCGACAAG 
AACGTGTTTG CTTCCCTAAA CTCAAGGACT GGAGACCTGT TCTGGCGTCA TGTGGACAAG 
ACTGGACCCG AGGGCCATAT TGATGCACTG CTTATGCATG GCCAGGATGC TGTTGTTGTT 
GTCGGGAATG GCCGTCTGCT TCGCTCCTGG GAGACTACAG TCGGAGGCTT GAAGTGGGAG 
ACTGTGCTTG ATTCTGGAAG TTTTCAGGCC GCTGCTTTAG TTGGAGTTCA AGATTATGTG 
AAGTACGTTG CTGTGCTGAA GAAATCGACA ATCGCTCTCC ATGACCTTTC AAGTGGCAGC 
CAAACATGGG TGGAAAACCT ACCTGACAGT GACACTGTTC AGTATCAAAC CATTTACTCT 
GGGGGGAATG GCCTAGTGTT TGTTTTGGGA GTTTTGCCAA ACTCTCACAT TGTTATTGTT 
GAATACAAAA TTGAAGATGG AGAAATCATG AACAAGAAAT CAGTTGAAGC CGCCTGGATG 
TCTAGCCTGG AGAGCAGCTG TGTAGTGGTC AGCTCAGGGA TCCTGATGTG TGTGGATCAG 
ATCACACAGT CCCTGTACAC TCAACCACTG CACTCTGCCG AACAAACAGA AATGAGACAG 
ATCCATCTTC AGACGCTAGA TTTGGAGGTG GCGTCTGGAT TCCAGCCTGT TTTGACATCC 
ACTCAGCCCA GTCCAGCACA TCCTCCGCTG GCTGAGTTCA TCCTGCAGCT CAGTCCAGAA 
CATCACATCT TGCTGCAGCT CAAAGACGGA CTTATCGCGC CGCTCCGAGA CTTCAGTCCA 
TCTTATCTTG CTGCTTTTGC CACATCTGGA GAGAAGACAG TTGTTGCTGT GATGTCCCCA 
AAGAATGATA CTGCCTGCAG CATCAATCTG TTCAGTGCTG ACACTGGCCG CAGGCACCTT 
GACACAACCA TTATCTACCA CACAGACCCT TACGGAGGAA AACCAAATAA ATTGTATGTC 
CACGCCTTCC TAAAAAAAGA TGACTCGGTG GGCTACAGAG TCATGGTACA GACGGAAGAC 
CTTACACTCA CATTTCTGCA GCAGCCTGGC AGGGTGGTCT GGATGAGGGA GGAGGCTCTG 
GCTGATGTGG TAAACATGGA GATGGTTGAT CTGCCACTTA CTGGGACACA GGCAGAGCTG 
GAAGGAGAGT TTGGGAAGAA AGCTGCCATT CAAGACGGAC TGTTACCCAT GGTTCTCAAG 
CGTCTCTCTT CCCAGTTCAT CTTGCTACAG GCCTGGATGG CTCATCTCTG GAAACTTTTC 
TATGATGCCC GAAAGCCCCG CAGCAGTGTG AAGAACGAGA TCACCATAGA CACACTGTCT 
CGTGACGAGT TCAACCTGCA GAAAATGATG GTAATGGTTA CTGCCTCTGG GAAGCTCTTT 
GGCATCGACA GTAGATCAGG GACTGTTTTG TGGAAGCAGT ATTTAGAAAA CATCAAGCCC 
AATTCGTTTT TCAAGCTCAT TGTGCAAAGG ACTACTGCCC ACTTTCCACA TCCACCACAG 
TGCACACTTC TTATAAAGGA CCAGGATACA GGGCTTGGAA GCCTGTATGT CTTCAATCCC 
ATCTTCGGCA AGAAAAGTCA AATTAGTGTC CCAGCGTTAC CCAGACCTAT CCTTCAGTCT 
CTTCTGTTAC CTGTCATCGA CCAGGACTAT TCTAAAGTCC TTCTACTTAT TGATGATCAG 
AACAAAGTCA CAGCATTTCC ATCTACTAAG AATATTCTGC AGCAGCTTCA GGAGACTGCA 
TCTTCCATAT TTTTCTACCT AGTGGACTCC AACCAGGGAA AGCTGTCGGG TTTTCGTCTG 
CGCACGGATT TGTCTACAGA ACTGATCTGG GAAGTTGTTA TCCCTACCGA GGTGCAGAAG 
ATAGTGGCTG TCAAAGGGAA GCGTGCAAAT GAACACGTGC ATTCCCAAGG CAGAGTCATG 
GGGGATCGTA GCGTGCTCTA CAAATATCTG AACCCTAATC TCTTGGCTGT GATAACTGAA 
AGCACAGACA CTCATCAGGA ACGCAGCTTT GTTGGAATCT TCCTGATTGA CGGTGTGACT 
GGCCGTATTG TGCATGAGGC TGTGCAGCGA AAGGCCAGAG GGCCAGTTCA CTGTGTGCAC 
TCTGAAAACT GGGTGATTTA TGTGTACTGG AACTCCAAGT TTCGCAGGAA TGAGTTTTCT 
GTGTTGGAGC TGTTTGAGGG AGCAGAGCTC TATAACAGCA CGGTGTTCAG CTCTCTGGAC 
AGACCTCATC CACCTCAGGT GCTGCAGCAG TCCTACATCT TTCCTGCTCC TATAAGCACT 
TTGGAGGCCA CACTTACAGA AAAAGGCATC ACCAGTCGAC ACCTTCTCGT GGGGCTGCCA 
TCAGGCAACA TTTTATCCTT ACCAAAGATG TTTTTGGACC CCAGGAGACC AGAGGTGGTC 
TCAGAACAGA GTCGGGAGGA AAATCTTATA CCATATGCTC CAGAAATGCC CATTCGTACA 
GAATGGTTCA TTAACTACAA CCAAACCGTA TCAAGAGTAA GAGGCATCTA TACTGCCCCA 
TCTGGCCTGG AGTCAACCTG TTTGGTGGTT GCATACGGCC TTGACATCTA CCAGACTAGA 
GTGTTCCCTT CCAAACAATT TGATGTCCTT AAGGATGACT ATGACTATGT CTTAATCAGC 
AGTGTCCTCC TCGGTCTCTT CTTTGCCACC ATGATCAGCA AACGTCTAGC GGAAGTGAAA 
CTGCTCAATA GGGCATGGCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162246.1
CDS	35..2938
Translation	MAWLVVRLAISVSLLYTASAVFEDQVGKFDWRQQYIGKVRFALFDTHSQASKKLLVATDKNVFASLNSRTGDLFWRHVDKTGPEGHIDALLMHGQDAVVVVGNGRLLRSWETTVGGLKWETVLDSGSFQAAALVGVQDYVKYVAVLKKSTIALHDLSSGSQTWVENLPDSDTVQYQTIYSGGNGLVFVLGVLPNSHIVIVEYKIEDGEIMNKKSVEAAWMSSLESSCVVVSSGILMCVDQITQSLYTQPLHSAEQTEMRQIHLQTLDLEVASGFQPVLTSTQPSPAHPPLAEFILQLSPEHHILLQLKDGLIAPLRDFSPSYLAAFATSGEKTVVAVMSPKNDTACSINLFSADTGRRHLDTTIIYHTDPYGGKPNKLYVHAFLKKDDSVGYRVMVQTEDLTLTFLQQPGRVVWMREEALADVVNMEMVDLPLTGTQAELEGEFGKKAAIQDGLLPMVLKRLSSQFILLQAWMAHLWKLFYDARKPRSSVKNEITIDTLSRDEFNLQKMMVMVTASGKLFGIDSRSGTVLWKQYLENIKPNSFFKLIVQRTTAHFPHPPQCTLLIKDQDTGLGSLYVFNPIFGKKSQISVPALPRPILQSLLLPVIDQDYSKVLLLIDDQNKVTAFPSTKNILQQLQETASSIFFYLVDSNQGKLSGFRLRTDLSTELIWEVVIPTEVQKIVAVKGKRANEHVHSQGRVMGDRSVLYKYLNPNLLAVITESTDTHQERSFVGIFLIDGVTGRIVHEAVQRKARGPVHCVHSENWVIYVYWNSKFRRNEFSVLELFEGAELYNSTVFSSLDRPHPPQVLQQSYIFPAPISTLEATLTEKGITSRHLLVGLPSGNILSLPKMFLDPRRPEVVSEQSREENLIPYAPEMPIRTEWFINYNQTVSRVRGIYTAPSGLESTCLVVAYGLDIYQTRVFPSKQFDVLKDDYDYVLISSVLLGLFFATMISKRLAEVKLLNRAWR

Target ORF information:

RefSeq Version	XM_005162189.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ER membrane protein complex subunit 1 (emc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005162189.3

ORF Insert Sequence:

ATGGCTTGGC TTGTAGTCAG ATTAGCAATA TCGGTGTCTT TATTATATAC TGCATCGGCG 
GTATTTGAAG ACCAGGTTGG AAAGTTTGAT TGGAGGCAAC AGTACATCGG CAAGGTGCGT 
TTTGCCTTGT TTGATACTCA TTCTCAAGCG TCTAAAAAGC TCCTGGTGGC CACCGACAAG 
AACGTGTTTG CTTCCCTAAA CTCAAGGACT GGAGACCTGT TCTGGCGTCA TGTGGACAAG 
ACTGGACCCG AGGGCCATAT TGATGCACTG CTTATGCATG GCCAGGATGC TGTTGTTGTT 
GTCGGGAATG GCCGTCTGCT TCGCTCCTGG GAGACTACAG TCGGAGGCTT GAAGTGGGAG 
ACTGTGCTTG ATTCTGGAAG TTTTCAGGCC GCTGCTTTAG TTGGAGTTCA AGATTATGTG 
AAGTACGTTG CTGTGCTGAA GAAATCGACA ATCGCTCTCC ATGACCTTTC AAGTGGCAGC 
CAAACATGGG TGGAAAACCT ACCTGACAGT GACACTGTTC AGTATCAAAC CATTTACTCT 
GGGGGGAATG GCCTAGTGTT TGTTTTGGGA GTTTTGCCAA ACTCTCACAT TGTTATTGTT 
GAATACAAAA TTGAAGATGG AGAAATCATG AACAAGAAAT CAGTTGAAGC CGCCTGGATG 
TCTAGCCTGG AGAGCAGCTG TGTAGTGGTC AGCTCAGGGA TCCTGATGTG TGTGGATCAG 
ATCACACAGT CCCTGTACAC TCAACCACTG CACTCTGCCG AACAAACAGA AATGAGACAG 
ATCCATCTTC AGACGCTAGA TTTGGAGGTG GCGTCTGGAT TCCAGCCTGT TTTGACATCC 
ACTCAGCCCA GTCCAGCACA TCCTCCGCTG GCTGAGTTCA TCCTGCAGCT CAGTCCAGAA 
CATCACATCT TGCTGCAGCT CAAAGACGGA CTTATCGCGC CGCTCCGAGA CTTCAGTCCA 
TCTTATCTTG CTGCTTTTGC CACATCTGGA GAGAAGACAG TTGTTGCTGT GATGTCCCCA 
AAGAATGATA CTGCCTGCAG CATCAATCTG TTCAGTGCTG ACACTGGCCG CAGGCACCTT 
GACACAACCA TTATCTACCA CACAGACCCT TACGGAGGAA AACCAAATAA ATTGTATGTC 
CACGCCTTCC TAAAAAAAGA TGACTCGGTG GGCTACAGAG TCATGGTACA GACGGAAGAC 
CTTACACTCA CATTTCTGCA GCAGCCTGGC AGGGTGGTCT GGATGAGGGA GGAGGCTCTG 
GCTGATGTGG TAAACATGGA GATGGTTGAT CTGCCACTTA CTGGGACACA GGCAGAGCTG 
GAAGGAGAGT TTGGGAAGAA AGCTGCCATT CAAGACGGAC TGTTACCCAT GGTTCTCAAG 
CGTCTCTCTT CCCAGTTCAT CTTGCTACAG GCCTGGATGG CTCATCTCTG GAAACTTTTC 
TATGATGCCC GAAAGCCCCG CAGCAGTGTG AAGAACGAGA TCACCATAGA CACACTGTCT 
CGTGACGAGT TCAACCTGCA GAAAATGATG GTAATGGTTA CTGCCTCTGG GAAGCTCTTT 
GGCATCGACA GTAGATCAGG GACTGTTTTG TGGAAGCAGT ATTTAGAAAA CATCAAGCCC 
AATTCGTTTT TCAAGCTCAT TGTGCAAAGG ACTACTGCCC ACTTTCCACA TCCACCACAG 
TGCACACTTC TTATAAAGGA CCAGGATACA GGGCTTGGAA GCCTGTATGT CTTCAATCCC 
ATCTTCGGCA AGAAAAGTCA AATTAGTGTC CCAGCGTTAC CCAGACCTAT CCTTCAGTCT 
CTTCTGTTAC CTGTCATCGA CCAGGACTAT TCTAAAGTCC TTCTACTTAT TGATGATCAG 
AACAAAGTCA CAGCATTTCC ATCTACTAAG AATATTCTGC AGCAGCTTCA GGAGACTGCA 
TCTTCCATAT TTTTCTACCT AGTGGACTCC AACCAGGGAA AGCTGTCGGG TTTTCGTCTG 
CGCACGGATT TGTCTACAGA ACTGATCTGG GAAGTTGTTA TCCCTACCGA GGTGCAGAAG 
ATAGTGGCTG TCAAAGGGAA GCGTGCAAAT GAACACGTGC ATTCCCAAGG CAGAGTCATG 
GGGGATCGTA GCGTGCTCTA CAAATATCTG AACCCTAATC TCTTGGCTGT GATAACTGAA 
AGCACAGACA CTCATCAGGA ACGCAGCTTT GTTGGAATCT TCCTGATTGA CGGTGTGACT 
GGCCGTATTG TGCATGAGGC TGTGCAGCGA AAGGCCAGAG GGCCAGTTCA CTGTGTGCAC 
TCTGAAAACT GGGTGATTTA TGTGTACTGG AACTCCAAGT TTCGCAGGAA TGAGTTTTCT 
GTGTTGGAGC TGTTTGAGGG AGCAGAGCTC TATAACAGCA CGGTGTTCAG CTCTCTGGAC 
AGACCTCATC CACCTCAGGT GCTGCAGCAG TCCTACATCT TTCCTGCTCC TATAAGCACT 
TTGGAGGCCA CACTTACAGA AAAAGGCATC ACCAGTCGAC ACCTTCTCGT GGGGCTGCCA 
TCAGGCAACA TTTTATCCTT ACCAAAGATG TTTTTGGACC CCAGGAGACC AGAGGTGGTC 
TCAGAACAGA GTCGGGAGGA AAATCTTATA CCATATGCTC CAGAAATGCC CATTCGTACA 
GAATGGTTCA TTAACTACAA CCAAACCGTA TCAAGAGTAA GAGGCATCTA TACTGCCCCA 
TCTGGCCTGG AGTCAACCTG TTTGGTGGTT GCATACGGCC TTGACATCTA CCAGACTAGA 
GTGTTCCCTT CCAAACAATT TGATGTCCTT AAGGATGACT ATGACTATGT CTTAATCAGC 
AGTGTCCTCC TCGGTCTCTT CTTTGCCACC ATGATCAGCA AACGTCTAGC GGAAGTGAAA 
CTGCTCAATA GGGCATGGCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMC1 ( XM_688678.9 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_693770.3 Danio rerio ER membrane protein complex subunit 1 (emc1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa27728
Clone ID Related Accession (Same CDS sequence)	XM_688678.9 , XM_688678.8
Accession Version	XM_688678.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ER membrane protein complex subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_688678.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGGC TTGTAGTCAG ATTAGCAATA TCGGTGTCTT TATTATATAC TGCATCGGCG 
GTATTTGAAG ACCAGGTTGG AAAGTTTGAT TGGAGGCAAC AGTACATCGG CAAGGTGCGT 
TTTGCCTTGT TTGATACTCA TTCTCAAGCG TCTAAAAAGC TCCTGGTGGC CACCGACAAG 
AACGTGTTTG CTTCCCTAAA CTCAAGGACT GGAGACCTGT TCTGGCGTCA TGTGGACAAG 
ACTGGACCCG AGGGCCATAT TGATGCACTG CTTATGCATG GCCAGGATGC TGTTGTTGTT 
GTCGGGAATG GCCGTCTGCT TCGCTCCTGG GAGACTACAG TCGGAGGCTT GAAGTGGGAG 
ACTGTGCTTG ATTCTGGAAG TTTTCAGGCC GCTGCTTTAG TTGGAGTTCA AGATTATGTG 
AAGTACGTTG CTGTGCTGAA GAAATCGACA ATCGCTCTCC ATGACCTTTC AAGTGGCAGC 
CAAACATGGG TGGAAAACCT ACCTGACAGT GACACTGTTC AGTATCAAAC CATTTACTCT 
GGGGGGAATG GCCTAGTGTT TGTTTTGGGA GTTTTGCCAA ACTCTCACAT TGTTATTGTT 
GAATACAAAA TTGAAGATGG AGAAATCATG AACAAGAAAT CAGTTGAAGC CGCCTGGATG 
TCTAGCCTGG AGAGCAGCTG TGTAGTGGTC AGCTCAGGGA TCCTGATGTG TGTGGATCAG 
ATCACACAGT CCCTGTACAC TCAACCACTG CACTCTGCCG AACAAACAGA AATGAGACAG 
ATCCATCTTC AGACGCTAGA TTTGGAGGTG GCGTCTGGAT TCCAGCCTGT TTTGACATCC 
ACTCAGCCCA GTCCAGCACA TCCTCCGCTG GCTGAGTTCA TCCTGCAGCT CAGTCCAGAA 
CATCACATCT TGCTGCAGCT CAAAGACGGA CTTATCGCGC CGCTCCGAGA CTTCAGTCCA 
TCTTATCTTG CTGCTTTTGC CACATCTGGA GAGAAGACAG TTGTTGCTGT GATGTCCCCA 
AAGAATGATA CTGCCTGCAG CATCAATCTG TTCAGTGCTG ACACTGGCCG CAGGCACCTT 
GACACAACCA TTATCTACCA CACAGACCCT TACGGAGGAA AACCAAATAA ATTGTATGTC 
CACGCCTTCC TAAAAAAAGA TGACTCGGTG GGCTACAGAG TCATGGTACA GACGGAAGAC 
CTTACACTCA CATTTCTGCA GCAGCCTGGC AGGGTGGTCT GGATGAGGGA GGAGGCTCTG 
GCTGATGTGG TAAACATGGA GATGGTTGAT CTGCCACTTA CTGGGACACA GGCAGAGCTG 
GAAGGAGAGT TTGGGAAGAA AGCTGACGGA CTGTTACCCA TGGTTCTCAA GCGTCTCTCT 
TCCCAGTTCA TCTTGCTACA GGCCTGGATG GCTCATCTCT GGAAACTTTT CTATGATGCC 
CGAAAGCCCC GCAGCAGTGT GAAGAACGAG ATCACCATAG ACACACTGTC TCGTGACGAG 
TTCAACCTGC AGAAAATGAT GGTAATGGTT ACTGCCTCTG GGAAGCTCTT TGGCATCGAC 
AGTAGATCAG GGACTGTTTT GTGGAAGCAG TATTTAGAAA ACATCAAGCC CAATTCGTTT 
TTCAAGCTCA TTGTGCAAAG GACTACTGCC CACTTTCCAC ATCCACCACA GTGCACACTT 
CTTATAAAGG ACCAGGATAC AGGGCTTGGA AGCCTGTATG TCTTCAATCC CATCTTCGGC 
AAGAAAAGTC AAATTAGTGT CCCAGCGTTA CCCAGACCTA TCCTTCAGTC TCTTCTGTTA 
CCTGTCATCG ACCAGGACTA TTCTAAAGTC CTTCTACTTA TTGATGATCA GAACAAAGTC 
ACAGCATTTC CATCTACTAA GAATATTCTG CAGCAGCTTC AGGAGACTGC ATCTTCCATA 
TTTTTCTACC TAGTGGACTC CAACCAGGGA AAGCTGTCGG GTTTTCGTCT GCGCACGGAT 
TTGTCTACAG AACTGATCTG GGAAGTTGTT ATCCCTACCG AGGTGCAGAA GATAGTGGCT 
GTCAAAGGGA AGCGTGCAAA TGAACACGTG CATTCCCAAG GCAGAGTCAT GGGGGATCGT 
AGCGTGCTCT ACAAATATCT GAACCCTAAT CTCTTGGCTG TGATAACTGA AAGCACAGAC 
ACTCATCAGG AACGCAGCTT TGTTGGAATC TTCCTGATTG ACGGTGTGAC TGGCCGTATT 
GTGCATGAGG CTGTGCAGCG AAAGGCCAGA GGGCCAGTTC ACTGTGTGCA CTCTGAAAAC 
TGGGTGATTT ATGTGTACTG GAACTCCAAG TTTCGCAGGA ATGAGTTTTC TGTGTTGGAG 
CTGTTTGAGG GAGCAGAGCT CTATAACAGC ACGGTGTTCA GCTCTCTGGA CAGACCTCAT 
CCACCTCAGG TGCTGCAGCA GTCCTACATC TTTCCTGCTC CTATAAGCAC TTTGGAGGCC 
ACACTTACAG AAAAAGGCAT CACCAGTCGA CACCTTCTCG TGGGGCTGCC ATCAGGCAAC 
ATTTTATCCT TACCAAAGAT GTTTTTGGAC CCCAGGAGAC CAGAGGTGGT CTCAGAACAG 
AGTCGGGAGG AAAATCTTAT ACCATATGCT CCAGAAATGC CCATTCGTAC AGAATGGTTC 
ATTAACTACA ACCAAACCGT ATCAAGAGTA AGAGGCATCT ATACTGCCCC ATCTGGCCTG 
GAGTCAACCT GTTTGGTGGT TGCATACGGC CTTGACATCT ACCAGACTAG AGTGTTCCCT 
TCCAAACAAT TTGATGTCCT TAAGGATGAC TATGACTATG TCTTAATCAG CAGTGTCCTC 
CTCGGTCTCT TCTTTGCCAC CATGATCAGC AAACGTCTAG CGGAAGTGAA ACTGCTCAAT 
AGGGCATGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_693770.3
CDS	36..2930
Translation	MAWLVVRLAISVSLLYTASAVFEDQVGKFDWRQQYIGKVRFALFDTHSQASKKLLVATDKNVFASLNSRTGDLFWRHVDKTGPEGHIDALLMHGQDAVVVVGNGRLLRSWETTVGGLKWETVLDSGSFQAAALVGVQDYVKYVAVLKKSTIALHDLSSGSQTWVENLPDSDTVQYQTIYSGGNGLVFVLGVLPNSHIVIVEYKIEDGEIMNKKSVEAAWMSSLESSCVVVSSGILMCVDQITQSLYTQPLHSAEQTEMRQIHLQTLDLEVASGFQPVLTSTQPSPAHPPLAEFILQLSPEHHILLQLKDGLIAPLRDFSPSYLAAFATSGEKTVVAVMSPKNDTACSINLFSADTGRRHLDTTIIYHTDPYGGKPNKLYVHAFLKKDDSVGYRVMVQTEDLTLTFLQQPGRVVWMREEALADVVNMEMVDLPLTGTQAELEGEFGKKADGLLPMVLKRLSSQFILLQAWMAHLWKLFYDARKPRSSVKNEITIDTLSRDEFNLQKMMVMVTASGKLFGIDSRSGTVLWKQYLENIKPNSFFKLIVQRTTAHFPHPPQCTLLIKDQDTGLGSLYVFNPIFGKKSQISVPALPRPILQSLLLPVIDQDYSKVLLLIDDQNKVTAFPSTKNILQQLQETASSIFFYLVDSNQGKLSGFRLRTDLSTELIWEVVIPTEVQKIVAVKGKRANEHVHSQGRVMGDRSVLYKYLNPNLLAVITESTDTHQERSFVGIFLIDGVTGRIVHEAVQRKARGPVHCVHSENWVIYVYWNSKFRRNEFSVLELFEGAELYNSTVFSSLDRPHPPQVLQQSYIFPAPISTLEATLTEKGITSRHLLVGLPSGNILSLPKMFLDPRRPEVVSEQSREENLIPYAPEMPIRTEWFINYNQTVSRVRGIYTAPSGLESTCLVVAYGLDIYQTRVFPSKQFDVLKDDYDYVLISSVLLGLFFATMISKRLAEVKLLNRAWR

Target ORF information:

RefSeq Version	XM_688678.9
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ER membrane protein complex subunit 1 (emc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_688678.9

ORF Insert Sequence:

ATGGCTTGGC TTGTAGTCAG ATTAGCAATA TCGGTGTCTT TATTATATAC TGCATCGGCG 
GTATTTGAAG ACCAGGTTGG AAAGTTTGAT TGGAGGCAAC AGTACATCGG CAAGGTGCGT 
TTTGCCTTGT TTGATACTCA TTCTCAAGCG TCTAAAAAGC TCCTGGTGGC CACCGACAAG 
AACGTGTTTG CTTCCCTAAA CTCAAGGACT GGAGACCTGT TCTGGCGTCA TGTGGACAAG 
ACTGGACCCG AGGGCCATAT TGATGCACTG CTTATGCATG GCCAGGATGC TGTTGTTGTT 
GTCGGGAATG GCCGTCTGCT TCGCTCCTGG GAGACTACAG TCGGAGGCTT GAAGTGGGAG 
ACTGTGCTTG ATTCTGGAAG TTTTCAGGCC GCTGCTTTAG TTGGAGTTCA AGATTATGTG 
AAGTACGTTG CTGTGCTGAA GAAATCGACA ATCGCTCTCC ATGACCTTTC AAGTGGCAGC 
CAAACATGGG TGGAAAACCT ACCTGACAGT GACACTGTTC AGTATCAAAC CATTTACTCT 
GGGGGGAATG GCCTAGTGTT TGTTTTGGGA GTTTTGCCAA ACTCTCACAT TGTTATTGTT 
GAATACAAAA TTGAAGATGG AGAAATCATG AACAAGAAAT CAGTTGAAGC CGCCTGGATG 
TCTAGCCTGG AGAGCAGCTG TGTAGTGGTC AGCTCAGGGA TCCTGATGTG TGTGGATCAG 
ATCACACAGT CCCTGTACAC TCAACCACTG CACTCTGCCG AACAAACAGA AATGAGACAG 
ATCCATCTTC AGACGCTAGA TTTGGAGGTG GCGTCTGGAT TCCAGCCTGT TTTGACATCC 
ACTCAGCCCA GTCCAGCACA TCCTCCGCTG GCTGAGTTCA TCCTGCAGCT CAGTCCAGAA 
CATCACATCT TGCTGCAGCT CAAAGACGGA CTTATCGCGC CGCTCCGAGA CTTCAGTCCA 
TCTTATCTTG CTGCTTTTGC CACATCTGGA GAGAAGACAG TTGTTGCTGT GATGTCCCCA 
AAGAATGATA CTGCCTGCAG CATCAATCTG TTCAGTGCTG ACACTGGCCG CAGGCACCTT 
GACACAACCA TTATCTACCA CACAGACCCT TACGGAGGAA AACCAAATAA ATTGTATGTC 
CACGCCTTCC TAAAAAAAGA TGACTCGGTG GGCTACAGAG TCATGGTACA GACGGAAGAC 
CTTACACTCA CATTTCTGCA GCAGCCTGGC AGGGTGGTCT GGATGAGGGA GGAGGCTCTG 
GCTGATGTGG TAAACATGGA GATGGTTGAT CTGCCACTTA CTGGGACACA GGCAGAGCTG 
GAAGGAGAGT TTGGGAAGAA AGCTGACGGA CTGTTACCCA TGGTTCTCAA GCGTCTCTCT 
TCCCAGTTCA TCTTGCTACA GGCCTGGATG GCTCATCTCT GGAAACTTTT CTATGATGCC 
CGAAAGCCCC GCAGCAGTGT GAAGAACGAG ATCACCATAG ACACACTGTC TCGTGACGAG 
TTCAACCTGC AGAAAATGAT GGTAATGGTT ACTGCCTCTG GGAAGCTCTT TGGCATCGAC 
AGTAGATCAG GGACTGTTTT GTGGAAGCAG TATTTAGAAA ACATCAAGCC CAATTCGTTT 
TTCAAGCTCA TTGTGCAAAG GACTACTGCC CACTTTCCAC ATCCACCACA GTGCACACTT 
CTTATAAAGG ACCAGGATAC AGGGCTTGGA AGCCTGTATG TCTTCAATCC CATCTTCGGC 
AAGAAAAGTC AAATTAGTGT CCCAGCGTTA CCCAGACCTA TCCTTCAGTC TCTTCTGTTA 
CCTGTCATCG ACCAGGACTA TTCTAAAGTC CTTCTACTTA TTGATGATCA GAACAAAGTC 
ACAGCATTTC CATCTACTAA GAATATTCTG CAGCAGCTTC AGGAGACTGC ATCTTCCATA 
TTTTTCTACC TAGTGGACTC CAACCAGGGA AAGCTGTCGG GTTTTCGTCT GCGCACGGAT 
TTGTCTACAG AACTGATCTG GGAAGTTGTT ATCCCTACCG AGGTGCAGAA GATAGTGGCT 
GTCAAAGGGA AGCGTGCAAA TGAACACGTG CATTCCCAAG GCAGAGTCAT GGGGGATCGT 
AGCGTGCTCT ACAAATATCT GAACCCTAAT CTCTTGGCTG TGATAACTGA AAGCACAGAC 
ACTCATCAGG AACGCAGCTT TGTTGGAATC TTCCTGATTG ACGGTGTGAC TGGCCGTATT 
GTGCATGAGG CTGTGCAGCG AAAGGCCAGA GGGCCAGTTC ACTGTGTGCA CTCTGAAAAC 
TGGGTGATTT ATGTGTACTG GAACTCCAAG TTTCGCAGGA ATGAGTTTTC TGTGTTGGAG 
CTGTTTGAGG GAGCAGAGCT CTATAACAGC ACGGTGTTCA GCTCTCTGGA CAGACCTCAT 
CCACCTCAGG TGCTGCAGCA GTCCTACATC TTTCCTGCTC CTATAAGCAC TTTGGAGGCC 
ACACTTACAG AAAAAGGCAT CACCAGTCGA CACCTTCTCG TGGGGCTGCC ATCAGGCAAC 
ATTTTATCCT TACCAAAGAT GTTTTTGGAC CCCAGGAGAC CAGAGGTGGT CTCAGAACAG 
AGTCGGGAGG AAAATCTTAT ACCATATGCT CCAGAAATGC CCATTCGTAC AGAATGGTTC 
ATTAACTACA ACCAAACCGT ATCAAGAGTA AGAGGCATCT ATACTGCCCC ATCTGGCCTG 
GAGTCAACCT GTTTGGTGGT TGCATACGGC CTTGACATCT ACCAGACTAG AGTGTTCCCT 
TCCAAACAAT TTGATGTCCT TAAGGATGAC TATGACTATG TCTTAATCAG CAGTGTCCTC 
CTCGGTCTCT TCTTTGCCAC CATGATCAGC AAACGTCTAG CGGAAGTGAA ACTGCTCAAT 
AGGGCATGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMC1 ( XM_688678.8 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_693770.3 Danio rerio ER membrane protein complex subunit 1 (emc1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa27728
Clone ID Related Accession (Same CDS sequence)	XM_688678.9 , XM_688678.8
Accession Version	XM_688678.8	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ER membrane protein complex subunit 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_688678.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGGC TTGTAGTCAG ATTAGCAATA TCGGTGTCTT TATTATATAC TGCATCGGCG 
GTATTTGAAG ACCAGGTTGG AAAGTTTGAT TGGAGGCAAC AGTACATCGG CAAGGTGCGT 
TTTGCCTTGT TTGATACTCA TTCTCAAGCG TCTAAAAAGC TCCTGGTGGC CACCGACAAG 
AACGTGTTTG CTTCCCTAAA CTCAAGGACT GGAGACCTGT TCTGGCGTCA TGTGGACAAG 
ACTGGACCCG AGGGCCATAT TGATGCACTG CTTATGCATG GCCAGGATGC TGTTGTTGTT 
GTCGGGAATG GCCGTCTGCT TCGCTCCTGG GAGACTACAG TCGGAGGCTT GAAGTGGGAG 
ACTGTGCTTG ATTCTGGAAG TTTTCAGGCC GCTGCTTTAG TTGGAGTTCA AGATTATGTG 
AAGTACGTTG CTGTGCTGAA GAAATCGACA ATCGCTCTCC ATGACCTTTC AAGTGGCAGC 
CAAACATGGG TGGAAAACCT ACCTGACAGT GACACTGTTC AGTATCAAAC CATTTACTCT 
GGGGGGAATG GCCTAGTGTT TGTTTTGGGA GTTTTGCCAA ACTCTCACAT TGTTATTGTT 
GAATACAAAA TTGAAGATGG AGAAATCATG AACAAGAAAT CAGTTGAAGC CGCCTGGATG 
TCTAGCCTGG AGAGCAGCTG TGTAGTGGTC AGCTCAGGGA TCCTGATGTG TGTGGATCAG 
ATCACACAGT CCCTGTACAC TCAACCACTG CACTCTGCCG AACAAACAGA AATGAGACAG 
ATCCATCTTC AGACGCTAGA TTTGGAGGTG GCGTCTGGAT TCCAGCCTGT TTTGACATCC 
ACTCAGCCCA GTCCAGCACA TCCTCCGCTG GCTGAGTTCA TCCTGCAGCT CAGTCCAGAA 
CATCACATCT TGCTGCAGCT CAAAGACGGA CTTATCGCGC CGCTCCGAGA CTTCAGTCCA 
TCTTATCTTG CTGCTTTTGC CACATCTGGA GAGAAGACAG TTGTTGCTGT GATGTCCCCA 
AAGAATGATA CTGCCTGCAG CATCAATCTG TTCAGTGCTG ACACTGGCCG CAGGCACCTT 
GACACAACCA TTATCTACCA CACAGACCCT TACGGAGGAA AACCAAATAA ATTGTATGTC 
CACGCCTTCC TAAAAAAAGA TGACTCGGTG GGCTACAGAG TCATGGTACA GACGGAAGAC 
CTTACACTCA CATTTCTGCA GCAGCCTGGC AGGGTGGTCT GGATGAGGGA GGAGGCTCTG 
GCTGATGTGG TAAACATGGA GATGGTTGAT CTGCCACTTA CTGGGACACA GGCAGAGCTG 
GAAGGAGAGT TTGGGAAGAA AGCTGACGGA CTGTTACCCA TGGTTCTCAA GCGTCTCTCT 
TCCCAGTTCA TCTTGCTACA GGCCTGGATG GCTCATCTCT GGAAACTTTT CTATGATGCC 
CGAAAGCCCC GCAGCAGTGT GAAGAACGAG ATCACCATAG ACACACTGTC TCGTGACGAG 
TTCAACCTGC AGAAAATGAT GGTAATGGTT ACTGCCTCTG GGAAGCTCTT TGGCATCGAC 
AGTAGATCAG GGACTGTTTT GTGGAAGCAG TATTTAGAAA ACATCAAGCC CAATTCGTTT 
TTCAAGCTCA TTGTGCAAAG GACTACTGCC CACTTTCCAC ATCCACCACA GTGCACACTT 
CTTATAAAGG ACCAGGATAC AGGGCTTGGA AGCCTGTATG TCTTCAATCC CATCTTCGGC 
AAGAAAAGTC AAATTAGTGT CCCAGCGTTA CCCAGACCTA TCCTTCAGTC TCTTCTGTTA 
CCTGTCATCG ACCAGGACTA TTCTAAAGTC CTTCTACTTA TTGATGATCA GAACAAAGTC 
ACAGCATTTC CATCTACTAA GAATATTCTG CAGCAGCTTC AGGAGACTGC ATCTTCCATA 
TTTTTCTACC TAGTGGACTC CAACCAGGGA AAGCTGTCGG GTTTTCGTCT GCGCACGGAT 
TTGTCTACAG AACTGATCTG GGAAGTTGTT ATCCCTACCG AGGTGCAGAA GATAGTGGCT 
GTCAAAGGGA AGCGTGCAAA TGAACACGTG CATTCCCAAG GCAGAGTCAT GGGGGATCGT 
AGCGTGCTCT ACAAATATCT GAACCCTAAT CTCTTGGCTG TGATAACTGA AAGCACAGAC 
ACTCATCAGG AACGCAGCTT TGTTGGAATC TTCCTGATTG ACGGTGTGAC TGGCCGTATT 
GTGCATGAGG CTGTGCAGCG AAAGGCCAGA GGGCCAGTTC ACTGTGTGCA CTCTGAAAAC 
TGGGTGATTT ATGTGTACTG GAACTCCAAG TTTCGCAGGA ATGAGTTTTC TGTGTTGGAG 
CTGTTTGAGG GAGCAGAGCT CTATAACAGC ACGGTGTTCA GCTCTCTGGA CAGACCTCAT 
CCACCTCAGG TGCTGCAGCA GTCCTACATC TTTCCTGCTC CTATAAGCAC TTTGGAGGCC 
ACACTTACAG AAAAAGGCAT CACCAGTCGA CACCTTCTCG TGGGGCTGCC ATCAGGCAAC 
ATTTTATCCT TACCAAAGAT GTTTTTGGAC CCCAGGAGAC CAGAGGTGGT CTCAGAACAG 
AGTCGGGAGG AAAATCTTAT ACCATATGCT CCAGAAATGC CCATTCGTAC AGAATGGTTC 
ATTAACTACA ACCAAACCGT ATCAAGAGTA AGAGGCATCT ATACTGCCCC ATCTGGCCTG 
GAGTCAACCT GTTTGGTGGT TGCATACGGC CTTGACATCT ACCAGACTAG AGTGTTCCCT 
TCCAAACAAT TTGATGTCCT TAAGGATGAC TATGACTATG TCTTAATCAG CAGTGTCCTC 
CTCGGTCTCT TCTTTGCCAC CATGATCAGC AAACGTCTAG CGGAAGTGAA ACTGCTCAAT 
AGGGCATGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_693770.3
CDS	35..2929
Translation	MAWLVVRLAISVSLLYTASAVFEDQVGKFDWRQQYIGKVRFALFDTHSQASKKLLVATDKNVFASLNSRTGDLFWRHVDKTGPEGHIDALLMHGQDAVVVVGNGRLLRSWETTVGGLKWETVLDSGSFQAAALVGVQDYVKYVAVLKKSTIALHDLSSGSQTWVENLPDSDTVQYQTIYSGGNGLVFVLGVLPNSHIVIVEYKIEDGEIMNKKSVEAAWMSSLESSCVVVSSGILMCVDQITQSLYTQPLHSAEQTEMRQIHLQTLDLEVASGFQPVLTSTQPSPAHPPLAEFILQLSPEHHILLQLKDGLIAPLRDFSPSYLAAFATSGEKTVVAVMSPKNDTACSINLFSADTGRRHLDTTIIYHTDPYGGKPNKLYVHAFLKKDDSVGYRVMVQTEDLTLTFLQQPGRVVWMREEALADVVNMEMVDLPLTGTQAELEGEFGKKADGLLPMVLKRLSSQFILLQAWMAHLWKLFYDARKPRSSVKNEITIDTLSRDEFNLQKMMVMVTASGKLFGIDSRSGTVLWKQYLENIKPNSFFKLIVQRTTAHFPHPPQCTLLIKDQDTGLGSLYVFNPIFGKKSQISVPALPRPILQSLLLPVIDQDYSKVLLLIDDQNKVTAFPSTKNILQQLQETASSIFFYLVDSNQGKLSGFRLRTDLSTELIWEVVIPTEVQKIVAVKGKRANEHVHSQGRVMGDRSVLYKYLNPNLLAVITESTDTHQERSFVGIFLIDGVTGRIVHEAVQRKARGPVHCVHSENWVIYVYWNSKFRRNEFSVLELFEGAELYNSTVFSSLDRPHPPQVLQQSYIFPAPISTLEATLTEKGITSRHLLVGLPSGNILSLPKMFLDPRRPEVVSEQSREENLIPYAPEMPIRTEWFINYNQTVSRVRGIYTAPSGLESTCLVVAYGLDIYQTRVFPSKQFDVLKDDYDYVLISSVLLGLFFATMISKRLAEVKLLNRAWR

Target ORF information:

RefSeq Version	XM_688678.8
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ER membrane protein complex subunit 1 (emc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_688678.8

ORF Insert Sequence:

ATGGCTTGGC TTGTAGTCAG ATTAGCAATA TCGGTGTCTT TATTATATAC TGCATCGGCG 
GTATTTGAAG ACCAGGTTGG AAAGTTTGAT TGGAGGCAAC AGTACATCGG CAAGGTGCGT 
TTTGCCTTGT TTGATACTCA TTCTCAAGCG TCTAAAAAGC TCCTGGTGGC CACCGACAAG 
AACGTGTTTG CTTCCCTAAA CTCAAGGACT GGAGACCTGT TCTGGCGTCA TGTGGACAAG 
ACTGGACCCG AGGGCCATAT TGATGCACTG CTTATGCATG GCCAGGATGC TGTTGTTGTT 
GTCGGGAATG GCCGTCTGCT TCGCTCCTGG GAGACTACAG TCGGAGGCTT GAAGTGGGAG 
ACTGTGCTTG ATTCTGGAAG TTTTCAGGCC GCTGCTTTAG TTGGAGTTCA AGATTATGTG 
AAGTACGTTG CTGTGCTGAA GAAATCGACA ATCGCTCTCC ATGACCTTTC AAGTGGCAGC 
CAAACATGGG TGGAAAACCT ACCTGACAGT GACACTGTTC AGTATCAAAC CATTTACTCT 
GGGGGGAATG GCCTAGTGTT TGTTTTGGGA GTTTTGCCAA ACTCTCACAT TGTTATTGTT 
GAATACAAAA TTGAAGATGG AGAAATCATG AACAAGAAAT CAGTTGAAGC CGCCTGGATG 
TCTAGCCTGG AGAGCAGCTG TGTAGTGGTC AGCTCAGGGA TCCTGATGTG TGTGGATCAG 
ATCACACAGT CCCTGTACAC TCAACCACTG CACTCTGCCG AACAAACAGA AATGAGACAG 
ATCCATCTTC AGACGCTAGA TTTGGAGGTG GCGTCTGGAT TCCAGCCTGT TTTGACATCC 
ACTCAGCCCA GTCCAGCACA TCCTCCGCTG GCTGAGTTCA TCCTGCAGCT CAGTCCAGAA 
CATCACATCT TGCTGCAGCT CAAAGACGGA CTTATCGCGC CGCTCCGAGA CTTCAGTCCA 
TCTTATCTTG CTGCTTTTGC CACATCTGGA GAGAAGACAG TTGTTGCTGT GATGTCCCCA 
AAGAATGATA CTGCCTGCAG CATCAATCTG TTCAGTGCTG ACACTGGCCG CAGGCACCTT 
GACACAACCA TTATCTACCA CACAGACCCT TACGGAGGAA AACCAAATAA ATTGTATGTC 
CACGCCTTCC TAAAAAAAGA TGACTCGGTG GGCTACAGAG TCATGGTACA GACGGAAGAC 
CTTACACTCA CATTTCTGCA GCAGCCTGGC AGGGTGGTCT GGATGAGGGA GGAGGCTCTG 
GCTGATGTGG TAAACATGGA GATGGTTGAT CTGCCACTTA CTGGGACACA GGCAGAGCTG 
GAAGGAGAGT TTGGGAAGAA AGCTGACGGA CTGTTACCCA TGGTTCTCAA GCGTCTCTCT 
TCCCAGTTCA TCTTGCTACA GGCCTGGATG GCTCATCTCT GGAAACTTTT CTATGATGCC 
CGAAAGCCCC GCAGCAGTGT GAAGAACGAG ATCACCATAG ACACACTGTC TCGTGACGAG 
TTCAACCTGC AGAAAATGAT GGTAATGGTT ACTGCCTCTG GGAAGCTCTT TGGCATCGAC 
AGTAGATCAG GGACTGTTTT GTGGAAGCAG TATTTAGAAA ACATCAAGCC CAATTCGTTT 
TTCAAGCTCA TTGTGCAAAG GACTACTGCC CACTTTCCAC ATCCACCACA GTGCACACTT 
CTTATAAAGG ACCAGGATAC AGGGCTTGGA AGCCTGTATG TCTTCAATCC CATCTTCGGC 
AAGAAAAGTC AAATTAGTGT CCCAGCGTTA CCCAGACCTA TCCTTCAGTC TCTTCTGTTA 
CCTGTCATCG ACCAGGACTA TTCTAAAGTC CTTCTACTTA TTGATGATCA GAACAAAGTC 
ACAGCATTTC CATCTACTAA GAATATTCTG CAGCAGCTTC AGGAGACTGC ATCTTCCATA 
TTTTTCTACC TAGTGGACTC CAACCAGGGA AAGCTGTCGG GTTTTCGTCT GCGCACGGAT 
TTGTCTACAG AACTGATCTG GGAAGTTGTT ATCCCTACCG AGGTGCAGAA GATAGTGGCT 
GTCAAAGGGA AGCGTGCAAA TGAACACGTG CATTCCCAAG GCAGAGTCAT GGGGGATCGT 
AGCGTGCTCT ACAAATATCT GAACCCTAAT CTCTTGGCTG TGATAACTGA AAGCACAGAC 
ACTCATCAGG AACGCAGCTT TGTTGGAATC TTCCTGATTG ACGGTGTGAC TGGCCGTATT 
GTGCATGAGG CTGTGCAGCG AAAGGCCAGA GGGCCAGTTC ACTGTGTGCA CTCTGAAAAC 
TGGGTGATTT ATGTGTACTG GAACTCCAAG TTTCGCAGGA ATGAGTTTTC TGTGTTGGAG 
CTGTTTGAGG GAGCAGAGCT CTATAACAGC ACGGTGTTCA GCTCTCTGGA CAGACCTCAT 
CCACCTCAGG TGCTGCAGCA GTCCTACATC TTTCCTGCTC CTATAAGCAC TTTGGAGGCC 
ACACTTACAG AAAAAGGCAT CACCAGTCGA CACCTTCTCG TGGGGCTGCC ATCAGGCAAC 
ATTTTATCCT TACCAAAGAT GTTTTTGGAC CCCAGGAGAC CAGAGGTGGT CTCAGAACAG 
AGTCGGGAGG AAAATCTTAT ACCATATGCT CCAGAAATGC CCATTCGTAC AGAATGGTTC 
ATTAACTACA ACCAAACCGT ATCAAGAGTA AGAGGCATCT ATACTGCCCC ATCTGGCCTG 
GAGTCAACCT GTTTGGTGGT TGCATACGGC CTTGACATCT ACCAGACTAG AGTGTTCCCT 
TCCAAACAAT TTGATGTCCT TAAGGATGAC TATGACTATG TCTTAATCAG CAGTGTCCTC 
CTCGGTCTCT TCTTTGCCAC CATGATCAGC AAACGTCTAG CGGAAGTGAA ACTGCTCAAT 
AGGGCATGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMC1 ( XM_021473641.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021329316.1 Danio rerio ER membrane protein complex subunit 1 (emc1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa146258
Clone ID Related Accession (Same CDS sequence)	XM_021473641.1
Accession Version	XM_021473641.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ER membrane protein complex subunit 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395144.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGGC TTGTAGTCAG ATTAGCAATA TCGGTGTCTT TATTATATAC TGCATCGGCG 
GTATTTGAAG ACCAGGTTGG AAAGTTTGAT TGGAGGCAAC AGTACATCGG CAAGGTGCGT 
TTTGCCTTGT TTGATACTCA TTCTCAAGCG TCTAAAAAGC TCCTGGTGGC CACCGACAAG 
AACGTGTTTG CTTCCCTAAA CTCAAGGACT GGAGACCTGT TCTGGCGTCA TGTGGACAAG 
ACTGGACCCG AGGGCCATAT TGATGCACTG CTTATGCATG GCCAGGATGC TGTTGTTGTT 
GTCGGGAATG GCCGTCTGCT TCGCTCCTGG GAGACTACAG TCGGAGGCTT GAAGTGGGAG 
ACTGTGCTTG ATTCTGGAAG TTTTCAGGCC GCTGCTTTAG TTGGAGTTCA AGATTATGTG 
AAGTACGTTG CTGTGCTGAA GAAATCGACA ATCGCTCTCC ATGACCTTTC AAGTGGCAGC 
CAAACATGGG TGGAAAACCT ACCTGACAGT GACACTGTTC AGTATCAAAC CATTTACTCT 
GGGGGGAATG GCCTAGTGTT TGTTTTGGGA GTTTTGCCAA ACTCTCACAT TGTTATTGTT 
GAATACAAAA TTGAAGATGG AGAAATCATG AACAAGAAAT CAGTTGAAGC CGCCTGGATG 
TCTAGCCTGG AGAGCAGCTG TGTAGTGGTC AGCTCAGGGA TCCTGATGTG TGTGGATCAG 
ATCACACAGT CCCTGTACAC TCAACCACTG CACTCTGCCG AACAAACAGA AATGAGACAG 
ATCCATCTTC AGACGCTAGA TTTGGAGGTG GCGCCTGGAT TCCAGCCTGT TTTGACATCC 
ACTCAGCCCA GTCCAGCACA TCCTCCGCTG GCTGAGTTCA TCCTGCAGCT CAGTCCAGAA 
CATCACATCT TGCTGCAGCT CAAAGACGGA CTTATCGTGC CGCTCCGAGA CTTCAGTCCA 
TCTTATCTTG CTGCTTTTGC CACATCTGGA GAGAAGACGG TTGTTGCTGT GATGTCCCCA 
AAGAATGATA CTGCCTGCAG CATCAATCTG TTCAGTGCTG ACACTGGCCG CAGGCACCTT 
GACACAACTA TTATCTACCA CACAGACCCT TATGGAGGAA AACCAAATAA ATTGTATGTC 
CACGCCTTCC TAAAAAAAGA TGACTCGGTG GGCTACAGAG TCATGGTACA GACGGAAGAC 
CTTACACTCA CATTTCTGCA GCAGCCTGGC AGGGTGGTCT GGATGAGGGA GGAGGCTCTG 
GCTGATGTGG TAAACATGGA GATGGTTGAT CTGCCACTCA CTGGGACACA GGCAGAGCTG 
GAAGGAGAGT TTGGGAAGAA AGCTGCCATT CAAGATGGAC TGTTACCCAT GGTTCTCAAG 
CGTCTCTCTT CCCAGTTCAT CTTGCTACAG GCCTGGATGG CTCATCTCTG GAAACTTTTC 
TATGATGCCC GAAAGCCCCG CAGCAGTGTG AAGAACGAGA TCACCATAGA CACACTGTCT 
CGTGATGAGT TCAACCTGCA GAAAATGATG GTGATGGTGA CTGCCTCTGG GAAGCTCTTT 
GGCATCGACA GTAGATCAGG GACTGTTTTG TGGAAGCAGT ATTTAGAAAA CATCAAGCCC 
AATTCGGTTT TCAAGCTCAT TGTGCAAAGG ACTACTGCCC ACTTTCCACA TCCACCACAG 
TGCACACTTC TTATAAAGGA CCAGGATACA GGGCTTGGAA GCCTTTATGT CTTCAATCCC 
ATCTTCGGCA AGAAAAGTCA AATTAGTGTC CCAGCGTTAC CCAGACCTAT CCTTCAGTCT 
CTTCTGTTAC CTGTCATCGA CCAGGACTAT TCTAAAGTCC TTCTACTTAT TGATGATCAG 
AACAAAGTCA CAGCATTTCC ATCTACTAAG AATATTCTGC AGCAGCTTCA GGAGACTGCA 
TCTTCCATAT TTTTCTACCT AGTGGACTCC AACCAGGGAA AGCTGTCGGG TTTTCGTCTG 
CGCACGGATT TGTCTACAGA ACTGATCTGG GAAGTTGTTA TCCCTACCGA GGTGCAGAAG 
ATAGTGGCTG TCAAAGGGAA GCGTGCAAAT GAACACGTGC ATTCCCAAGG CAGAGTCATG 
GGGGATCGTA GTGTGCTCTA CAAATATCTG AACCCTAATC TCTTGGCTGT GATAACTGAA 
AGCACAGACA CTCATCAGGA ACGCAGCTTT GTTGGAATCT TCCTGATTGA CGGTGTGACT 
GGCCGTATTG TGCATGAGGC TGTGCAGCGA AAGGCCAGAG GGCCAGTTCA CTGTGTGCAC 
TCTGAAAACT GGGTGGTTTA TGTGTACTGG AACTCCAAGT TTCGCAGGAA TGAGTTTTCT 
GTGTTGGAGC TGTTTGAGGG AGCAGAGCTC TATAACAGCA CGGTGTTCAG CTCTCTGGAC 
AGACCTCATC CACCTCAGGT GCTGCAGCAG TCCTACATCT TTCCTGCTCC TATAAGCACT 
TTGGAGGCCA CACTTACAGA AAAAGGCATC ACCAGTCGAC ACCTTCTCGT GGGGCTGCCA 
TCAGGCAACA TTTTATCCTT ACCAAAGATG TTTTTGGACC CCAGGAGACC AGAGGTGGTC 
TCAGAACAGA GTCGGGAGGA AAATCTTATA CCATATGCTC CAGAAATGCC CATTCGTACA 
GAATGGTTCA TTAACTACAA CCAAACCGTA TCAAGAGTAA GAGGCATCTA TACTGCCCCA 
TCTGGCCTGG AGTCAACCTG TTTGGTGGTT GCATACGGCC TTGACATCTA CCAGACTAGA 
GTGTTCCCTT CCAAACAATT TGATGTCCTT AAGGATGACT ATGACTATGT CTTAATCAGC 
AGTGTCCTCC TCGGTCTCTT CTTTGCCACC ATGATCAGCA AACGTCTAGC GGAAGTGAAA 
CTGCTCAATA GGGCATGGCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329316.1
CDS	36..2939
Translation	MAWLVVRLAISVSLLYTASAVFEDQVGKFDWRQQYIGKVRFALFDTHSQASKKLLVATDKNVFASLNSRTGDLFWRHVDKTGPEGHIDALLMHGQDAVVVVGNGRLLRSWETTVGGLKWETVLDSGSFQAAALVGVQDYVKYVAVLKKSTIALHDLSSGSQTWVENLPDSDTVQYQTIYSGGNGLVFVLGVLPNSHIVIVEYKIEDGEIMNKKSVEAAWMSSLESSCVVVSSGILMCVDQITQSLYTQPLHSAEQTEMRQIHLQTLDLEVAPGFQPVLTSTQPSPAHPPLAEFILQLSPEHHILLQLKDGLIVPLRDFSPSYLAAFATSGEKTVVAVMSPKNDTACSINLFSADTGRRHLDTTIIYHTDPYGGKPNKLYVHAFLKKDDSVGYRVMVQTEDLTLTFLQQPGRVVWMREEALADVVNMEMVDLPLTGTQAELEGEFGKKAAIQDGLLPMVLKRLSSQFILLQAWMAHLWKLFYDARKPRSSVKNEITIDTLSRDEFNLQKMMVMVTASGKLFGIDSRSGTVLWKQYLENIKPNSVFKLIVQRTTAHFPHPPQCTLLIKDQDTGLGSLYVFNPIFGKKSQISVPALPRPILQSLLLPVIDQDYSKVLLLIDDQNKVTAFPSTKNILQQLQETASSIFFYLVDSNQGKLSGFRLRTDLSTELIWEVVIPTEVQKIVAVKGKRANEHVHSQGRVMGDRSVLYKYLNPNLLAVITESTDTHQERSFVGIFLIDGVTGRIVHEAVQRKARGPVHCVHSENWVVYVYWNSKFRRNEFSVLELFEGAELYNSTVFSSLDRPHPPQVLQQSYIFPAPISTLEATLTEKGITSRHLLVGLPSGNILSLPKMFLDPRRPEVVSEQSREENLIPYAPEMPIRTEWFINYNQTVSRVRGIYTAPSGLESTCLVVAYGLDIYQTRVFPSKQFDVLKDDYDYVLISSVLLGLFFATMISKRLAEVKLLNRAWR

Target ORF information:

RefSeq Version	XM_021473641.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ER membrane protein complex subunit 1 (emc1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473641.1

ORF Insert Sequence:

ATGGCTTGGC TTGTAGTCAG ATTAGCAATA TCGGTGTCTT TATTATATAC TGCATCGGCG 
GTATTTGAAG ACCAGGTTGG AAAGTTTGAT TGGAGGCAAC AGTACATCGG CAAGGTGCGT 
TTTGCCTTGT TTGATACTCA TTCTCAAGCG TCTAAAAAGC TCCTGGTGGC CACCGACAAG 
AACGTGTTTG CTTCCCTAAA CTCAAGGACT GGAGACCTGT TCTGGCGTCA TGTGGACAAG 
ACTGGACCCG AGGGCCATAT TGATGCACTG CTTATGCATG GCCAGGATGC TGTTGTTGTT 
GTCGGGAATG GCCGTCTGCT TCGCTCCTGG GAGACTACAG TCGGAGGCTT GAAGTGGGAG 
ACTGTGCTTG ATTCTGGAAG TTTTCAGGCC GCTGCTTTAG TTGGAGTTCA AGATTATGTG 
AAGTACGTTG CTGTGCTGAA GAAATCGACA ATCGCTCTCC ATGACCTTTC AAGTGGCAGC 
CAAACATGGG TGGAAAACCT ACCTGACAGT GACACTGTTC AGTATCAAAC CATTTACTCT 
GGGGGGAATG GCCTAGTGTT TGTTTTGGGA GTTTTGCCAA ACTCTCACAT TGTTATTGTT 
GAATACAAAA TTGAAGATGG AGAAATCATG AACAAGAAAT CAGTTGAAGC CGCCTGGATG 
TCTAGCCTGG AGAGCAGCTG TGTAGTGGTC AGCTCAGGGA TCCTGATGTG TGTGGATCAG 
ATCACACAGT CCCTGTACAC TCAACCACTG CACTCTGCCG AACAAACAGA AATGAGACAG 
ATCCATCTTC AGACGCTAGA TTTGGAGGTG GCGCCTGGAT TCCAGCCTGT TTTGACATCC 
ACTCAGCCCA GTCCAGCACA TCCTCCGCTG GCTGAGTTCA TCCTGCAGCT CAGTCCAGAA 
CATCACATCT TGCTGCAGCT CAAAGACGGA CTTATCGTGC CGCTCCGAGA CTTCAGTCCA 
TCTTATCTTG CTGCTTTTGC CACATCTGGA GAGAAGACGG TTGTTGCTGT GATGTCCCCA 
AAGAATGATA CTGCCTGCAG CATCAATCTG TTCAGTGCTG ACACTGGCCG CAGGCACCTT 
GACACAACTA TTATCTACCA CACAGACCCT TATGGAGGAA AACCAAATAA ATTGTATGTC 
CACGCCTTCC TAAAAAAAGA TGACTCGGTG GGCTACAGAG TCATGGTACA GACGGAAGAC 
CTTACACTCA CATTTCTGCA GCAGCCTGGC AGGGTGGTCT GGATGAGGGA GGAGGCTCTG 
GCTGATGTGG TAAACATGGA GATGGTTGAT CTGCCACTCA CTGGGACACA GGCAGAGCTG 
GAAGGAGAGT TTGGGAAGAA AGCTGCCATT CAAGATGGAC TGTTACCCAT GGTTCTCAAG 
CGTCTCTCTT CCCAGTTCAT CTTGCTACAG GCCTGGATGG CTCATCTCTG GAAACTTTTC 
TATGATGCCC GAAAGCCCCG CAGCAGTGTG AAGAACGAGA TCACCATAGA CACACTGTCT 
CGTGATGAGT TCAACCTGCA GAAAATGATG GTGATGGTGA CTGCCTCTGG GAAGCTCTTT 
GGCATCGACA GTAGATCAGG GACTGTTTTG TGGAAGCAGT ATTTAGAAAA CATCAAGCCC 
AATTCGGTTT TCAAGCTCAT TGTGCAAAGG ACTACTGCCC ACTTTCCACA TCCACCACAG 
TGCACACTTC TTATAAAGGA CCAGGATACA GGGCTTGGAA GCCTTTATGT CTTCAATCCC 
ATCTTCGGCA AGAAAAGTCA AATTAGTGTC CCAGCGTTAC CCAGACCTAT CCTTCAGTCT 
CTTCTGTTAC CTGTCATCGA CCAGGACTAT TCTAAAGTCC TTCTACTTAT TGATGATCAG 
AACAAAGTCA CAGCATTTCC ATCTACTAAG AATATTCTGC AGCAGCTTCA GGAGACTGCA 
TCTTCCATAT TTTTCTACCT AGTGGACTCC AACCAGGGAA AGCTGTCGGG TTTTCGTCTG 
CGCACGGATT TGTCTACAGA ACTGATCTGG GAAGTTGTTA TCCCTACCGA GGTGCAGAAG 
ATAGTGGCTG TCAAAGGGAA GCGTGCAAAT GAACACGTGC ATTCCCAAGG CAGAGTCATG 
GGGGATCGTA GTGTGCTCTA CAAATATCTG AACCCTAATC TCTTGGCTGT GATAACTGAA 
AGCACAGACA CTCATCAGGA ACGCAGCTTT GTTGGAATCT TCCTGATTGA CGGTGTGACT 
GGCCGTATTG TGCATGAGGC TGTGCAGCGA AAGGCCAGAG GGCCAGTTCA CTGTGTGCAC 
TCTGAAAACT GGGTGGTTTA TGTGTACTGG AACTCCAAGT TTCGCAGGAA TGAGTTTTCT 
GTGTTGGAGC TGTTTGAGGG AGCAGAGCTC TATAACAGCA CGGTGTTCAG CTCTCTGGAC 
AGACCTCATC CACCTCAGGT GCTGCAGCAG TCCTACATCT TTCCTGCTCC TATAAGCACT 
TTGGAGGCCA CACTTACAGA AAAAGGCATC ACCAGTCGAC ACCTTCTCGT GGGGCTGCCA 
TCAGGCAACA TTTTATCCTT ACCAAAGATG TTTTTGGACC CCAGGAGACC AGAGGTGGTC 
TCAGAACAGA GTCGGGAGGA AAATCTTATA CCATATGCTC CAGAAATGCC CATTCGTACA 
GAATGGTTCA TTAACTACAA CCAAACCGTA TCAAGAGTAA GAGGCATCTA TACTGCCCCA 
TCTGGCCTGG AGTCAACCTG TTTGGTGGTT GCATACGGCC TTGACATCTA CCAGACTAGA 
GTGTTCCCTT CCAAACAATT TGATGTCCTT AAGGATGACT ATGACTATGT CTTAATCAGC 
AGTGTCCTCC TCGGTCTCTT CTTTGCCACC ATGATCAGCA AACGTCTAGC GGAAGTGAAA 
CTGCTCAATA GGGCATGGCG ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EMC1 ( XM_021473642.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021329317.1 Danio rerio ER membrane protein complex subunit 1 (emc1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa146259
Clone ID Related Accession (Same CDS sequence)	XM_021473642.1
Accession Version	XM_021473642.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2895bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ER membrane protein complex subunit 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395144.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTGGC TTGTAGTCAG ATTAGCAATA TCGGTGTCTT TATTATATAC TGCATCGGCG 
GTATTTGAAG ACCAGGTTGG AAAGTTTGAT TGGAGGCAAC AGTACATCGG CAAGGTGCGT 
TTTGCCTTGT TTGATACTCA TTCTCAAGCG TCTAAAAAGC TCCTGGTGGC CACCGACAAG 
AACGTGTTTG CTTCCCTAAA CTCAAGGACT GGAGACCTGT TCTGGCGTCA TGTGGACAAG 
ACTGGACCCG AGGGCCATAT TGATGCACTG CTTATGCATG GCCAGGATGC TGTTGTTGTT 
GTCGGGAATG GCCGTCTGCT TCGCTCCTGG GAGACTACAG TCGGAGGCTT GAAGTGGGAG 
ACTGTGCTTG ATTCTGGAAG TTTTCAGGCC GCTGCTTTAG TTGGAGTTCA AGATTATGTG 
AAGTACGTTG CTGTGCTGAA GAAATCGACA ATCGCTCTCC ATGACCTTTC AAGTGGCAGC 
CAAACATGGG TGGAAAACCT ACCTGACAGT GACACTGTTC AGTATCAAAC CATTTACTCT 
GGGGGGAATG GCCTAGTGTT TGTTTTGGGA GTTTTGCCAA ACTCTCACAT TGTTATTGTT 
GAATACAAAA TTGAAGATGG AGAAATCATG AACAAGAAAT CAGTTGAAGC CGCCTGGATG 
TCTAGCCTGG AGAGCAGCTG TGTAGTGGTC AGCTCAGGGA TCCTGATGTG TGTGGATCAG 
ATCACACAGT CCCTGTACAC TCAACCACTG CACTCTGCCG AACAAACAGA AATGAGACAG 
ATCCATCTTC AGACGCTAGA TTTGGAGGTG GCGCCTGGAT TCCAGCCTGT TTTGACATCC 
ACTCAGCCCA GTCCAGCACA TCCTCCGCTG GCTGAGTTCA TCCTGCAGCT CAGTCCAGAA 
CATCACATCT TGCTGCAGCT CAAAGACGGA CTTATCGTGC CGCTCCGAGA CTTCAGTCCA 
TCTTATCTTG CTGCTTTTGC CACATCTGGA GAGAAGACGG TTGTTGCTGT GATGTCCCCA 
AAGAATGATA CTGCCTGCAG CATCAATCTG TTCAGTGCTG ACACTGGCCG CAGGCACCTT 
GACACAACTA TTATCTACCA CACAGACCCT TATGGAGGAA AACCAAATAA ATTGTATGTC 
CACGCCTTCC TAAAAAAAGA TGACTCGGTG GGCTACAGAG TCATGGTACA GACGGAAGAC 
CTTACACTCA CATTTCTGCA GCAGCCTGGC AGGGTGGTCT GGATGAGGGA GGAGGCTCTG 
GCTGATGTGG TAAACATGGA GATGGTTGAT CTGCCACTCA CTGGGACACA GGCAGAGCTG 
GAAGGAGAGT TTGGGAAGAA AGCTGATGGA CTGTTACCCA TGGTTCTCAA GCGTCTCTCT 
TCCCAGTTCA TCTTGCTACA GGCCTGGATG GCTCATCTCT GGAAACTTTT CTATGATGCC 
CGAAAGCCCC GCAGCAGTGT GAAGAACGAG ATCACCATAG ACACACTGTC TCGTGATGAG 
TTCAACCTGC AGAAAATGAT GGTGATGGTG ACTGCCTCTG GGAAGCTCTT TGGCATCGAC 
AGTAGATCAG GGACTGTTTT GTGGAAGCAG TATTTAGAAA ACATCAAGCC CAATTCGGTT 
TTCAAGCTCA TTGTGCAAAG GACTACTGCC CACTTTCCAC ATCCACCACA GTGCACACTT 
CTTATAAAGG ACCAGGATAC AGGGCTTGGA AGCCTTTATG TCTTCAATCC CATCTTCGGC 
AAGAAAAGTC AAATTAGTGT CCCAGCGTTA CCCAGACCTA TCCTTCAGTC TCTTCTGTTA 
CCTGTCATCG ACCAGGACTA TTCTAAAGTC CTTCTACTTA TTGATGATCA GAACAAAGTC 
ACAGCATTTC CATCTACTAA GAATATTCTG CAGCAGCTTC AGGAGACTGC ATCTTCCATA 
TTTTTCTACC TAGTGGACTC CAACCAGGGA AAGCTGTCGG GTTTTCGTCT GCGCACGGAT 
TTGTCTACAG AACTGATCTG GGAAGTTGTT ATCCCTACCG AGGTGCAGAA GATAGTGGCT 
GTCAAAGGGA AGCGTGCAAA TGAACACGTG CATTCCCAAG GCAGAGTCAT GGGGGATCGT 
AGTGTGCTCT ACAAATATCT GAACCCTAAT CTCTTGGCTG TGATAACTGA AAGCACAGAC 
ACTCATCAGG AACGCAGCTT TGTTGGAATC TTCCTGATTG ACGGTGTGAC TGGCCGTATT 
GTGCATGAGG CTGTGCAGCG AAAGGCCAGA GGGCCAGTTC ACTGTGTGCA CTCTGAAAAC 
TGGGTGGTTT ATGTGTACTG GAACTCCAAG TTTCGCAGGA ATGAGTTTTC TGTGTTGGAG 
CTGTTTGAGG GAGCAGAGCT CTATAACAGC ACGGTGTTCA GCTCTCTGGA CAGACCTCAT 
CCACCTCAGG TGCTGCAGCA GTCCTACATC TTTCCTGCTC CTATAAGCAC TTTGGAGGCC 
ACACTTACAG AAAAAGGCAT CACCAGTCGA CACCTTCTCG TGGGGCTGCC ATCAGGCAAC 
ATTTTATCCT TACCAAAGAT GTTTTTGGAC CCCAGGAGAC CAGAGGTGGT CTCAGAACAG 
AGTCGGGAGG AAAATCTTAT ACCATATGCT CCAGAAATGC CCATTCGTAC AGAATGGTTC 
ATTAACTACA ACCAAACCGT ATCAAGAGTA AGAGGCATCT ATACTGCCCC ATCTGGCCTG 
GAGTCAACCT GTTTGGTGGT TGCATACGGC CTTGACATCT ACCAGACTAG AGTGTTCCCT 
TCCAAACAAT TTGATGTCCT TAAGGATGAC TATGACTATG TCTTAATCAG CAGTGTCCTC 
CTCGGTCTCT TCTTTGCCAC CATGATCAGC AAACGTCTAG CGGAAGTGAA ACTGCTCAAT 
AGGGCATGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329317.1
CDS	36..2930
Translation	MAWLVVRLAISVSLLYTASAVFEDQVGKFDWRQQYIGKVRFALFDTHSQASKKLLVATDKNVFASLNSRTGDLFWRHVDKTGPEGHIDALLMHGQDAVVVVGNGRLLRSWETTVGGLKWETVLDSGSFQAAALVGVQDYVKYVAVLKKSTIALHDLSSGSQTWVENLPDSDTVQYQTIYSGGNGLVFVLGVLPNSHIVIVEYKIEDGEIMNKKSVEAAWMSSLESSCVVVSSGILMCVDQITQSLYTQPLHSAEQTEMRQIHLQTLDLEVAPGFQPVLTSTQPSPAHPPLAEFILQLSPEHHILLQLKDGLIVPLRDFSPSYLAAFATSGEKTVVAVMSPKNDTACSINLFSADTGRRHLDTTIIYHTDPYGGKPNKLYVHAFLKKDDSVGYRVMVQTEDLTLTFLQQPGRVVWMREEALADVVNMEMVDLPLTGTQAELEGEFGKKADGLLPMVLKRLSSQFILLQAWMAHLWKLFYDARKPRSSVKNEITIDTLSRDEFNLQKMMVMVTASGKLFGIDSRSGTVLWKQYLENIKPNSVFKLIVQRTTAHFPHPPQCTLLIKDQDTGLGSLYVFNPIFGKKSQISVPALPRPILQSLLLPVIDQDYSKVLLLIDDQNKVTAFPSTKNILQQLQETASSIFFYLVDSNQGKLSGFRLRTDLSTELIWEVVIPTEVQKIVAVKGKRANEHVHSQGRVMGDRSVLYKYLNPNLLAVITESTDTHQERSFVGIFLIDGVTGRIVHEAVQRKARGPVHCVHSENWVVYVYWNSKFRRNEFSVLELFEGAELYNSTVFSSLDRPHPPQVLQQSYIFPAPISTLEATLTEKGITSRHLLVGLPSGNILSLPKMFLDPRRPEVVSEQSREENLIPYAPEMPIRTEWFINYNQTVSRVRGIYTAPSGLESTCLVVAYGLDIYQTRVFPSKQFDVLKDDYDYVLISSVLLGLFFATMISKRLAEVKLLNRAWR

Target ORF information:

RefSeq Version	XM_021473642.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ER membrane protein complex subunit 1 (emc1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473642.1

ORF Insert Sequence:

ATGGCTTGGC TTGTAGTCAG ATTAGCAATA TCGGTGTCTT TATTATATAC TGCATCGGCG 
GTATTTGAAG ACCAGGTTGG AAAGTTTGAT TGGAGGCAAC AGTACATCGG CAAGGTGCGT 
TTTGCCTTGT TTGATACTCA TTCTCAAGCG TCTAAAAAGC TCCTGGTGGC CACCGACAAG 
AACGTGTTTG CTTCCCTAAA CTCAAGGACT GGAGACCTGT TCTGGCGTCA TGTGGACAAG 
ACTGGACCCG AGGGCCATAT TGATGCACTG CTTATGCATG GCCAGGATGC TGTTGTTGTT 
GTCGGGAATG GCCGTCTGCT TCGCTCCTGG GAGACTACAG TCGGAGGCTT GAAGTGGGAG 
ACTGTGCTTG ATTCTGGAAG TTTTCAGGCC GCTGCTTTAG TTGGAGTTCA AGATTATGTG 
AAGTACGTTG CTGTGCTGAA GAAATCGACA ATCGCTCTCC ATGACCTTTC AAGTGGCAGC 
CAAACATGGG TGGAAAACCT ACCTGACAGT GACACTGTTC AGTATCAAAC CATTTACTCT 
GGGGGGAATG GCCTAGTGTT TGTTTTGGGA GTTTTGCCAA ACTCTCACAT TGTTATTGTT 
GAATACAAAA TTGAAGATGG AGAAATCATG AACAAGAAAT CAGTTGAAGC CGCCTGGATG 
TCTAGCCTGG AGAGCAGCTG TGTAGTGGTC AGCTCAGGGA TCCTGATGTG TGTGGATCAG 
ATCACACAGT CCCTGTACAC TCAACCACTG CACTCTGCCG AACAAACAGA AATGAGACAG 
ATCCATCTTC AGACGCTAGA TTTGGAGGTG GCGCCTGGAT TCCAGCCTGT TTTGACATCC 
ACTCAGCCCA GTCCAGCACA TCCTCCGCTG GCTGAGTTCA TCCTGCAGCT CAGTCCAGAA 
CATCACATCT TGCTGCAGCT CAAAGACGGA CTTATCGTGC CGCTCCGAGA CTTCAGTCCA 
TCTTATCTTG CTGCTTTTGC CACATCTGGA GAGAAGACGG TTGTTGCTGT GATGTCCCCA 
AAGAATGATA CTGCCTGCAG CATCAATCTG TTCAGTGCTG ACACTGGCCG CAGGCACCTT 
GACACAACTA TTATCTACCA CACAGACCCT TATGGAGGAA AACCAAATAA ATTGTATGTC 
CACGCCTTCC TAAAAAAAGA TGACTCGGTG GGCTACAGAG TCATGGTACA GACGGAAGAC 
CTTACACTCA CATTTCTGCA GCAGCCTGGC AGGGTGGTCT GGATGAGGGA GGAGGCTCTG 
GCTGATGTGG TAAACATGGA GATGGTTGAT CTGCCACTCA CTGGGACACA GGCAGAGCTG 
GAAGGAGAGT TTGGGAAGAA AGCTGATGGA CTGTTACCCA TGGTTCTCAA GCGTCTCTCT 
TCCCAGTTCA TCTTGCTACA GGCCTGGATG GCTCATCTCT GGAAACTTTT CTATGATGCC 
CGAAAGCCCC GCAGCAGTGT GAAGAACGAG ATCACCATAG ACACACTGTC TCGTGATGAG 
TTCAACCTGC AGAAAATGAT GGTGATGGTG ACTGCCTCTG GGAAGCTCTT TGGCATCGAC 
AGTAGATCAG GGACTGTTTT GTGGAAGCAG TATTTAGAAA ACATCAAGCC CAATTCGGTT 
TTCAAGCTCA TTGTGCAAAG GACTACTGCC CACTTTCCAC ATCCACCACA GTGCACACTT 
CTTATAAAGG ACCAGGATAC AGGGCTTGGA AGCCTTTATG TCTTCAATCC CATCTTCGGC 
AAGAAAAGTC AAATTAGTGT CCCAGCGTTA CCCAGACCTA TCCTTCAGTC TCTTCTGTTA 
CCTGTCATCG ACCAGGACTA TTCTAAAGTC CTTCTACTTA TTGATGATCA GAACAAAGTC 
ACAGCATTTC CATCTACTAA GAATATTCTG CAGCAGCTTC AGGAGACTGC ATCTTCCATA 
TTTTTCTACC TAGTGGACTC CAACCAGGGA AAGCTGTCGG GTTTTCGTCT GCGCACGGAT 
TTGTCTACAG AACTGATCTG GGAAGTTGTT ATCCCTACCG AGGTGCAGAA GATAGTGGCT 
GTCAAAGGGA AGCGTGCAAA TGAACACGTG CATTCCCAAG GCAGAGTCAT GGGGGATCGT 
AGTGTGCTCT ACAAATATCT GAACCCTAAT CTCTTGGCTG TGATAACTGA AAGCACAGAC 
ACTCATCAGG AACGCAGCTT TGTTGGAATC TTCCTGATTG ACGGTGTGAC TGGCCGTATT 
GTGCATGAGG CTGTGCAGCG AAAGGCCAGA GGGCCAGTTC ACTGTGTGCA CTCTGAAAAC 
TGGGTGGTTT ATGTGTACTG GAACTCCAAG TTTCGCAGGA ATGAGTTTTC TGTGTTGGAG 
CTGTTTGAGG GAGCAGAGCT CTATAACAGC ACGGTGTTCA GCTCTCTGGA CAGACCTCAT 
CCACCTCAGG TGCTGCAGCA GTCCTACATC TTTCCTGCTC CTATAAGCAC TTTGGAGGCC 
ACACTTACAG AAAAAGGCAT CACCAGTCGA CACCTTCTCG TGGGGCTGCC ATCAGGCAAC 
ATTTTATCCT TACCAAAGAT GTTTTTGGAC CCCAGGAGAC CAGAGGTGGT CTCAGAACAG 
AGTCGGGAGG AAAATCTTAT ACCATATGCT CCAGAAATGC CCATTCGTAC AGAATGGTTC 
ATTAACTACA ACCAAACCGT ATCAAGAGTA AGAGGCATCT ATACTGCCCC ATCTGGCCTG 
GAGTCAACCT GTTTGGTGGT TGCATACGGC CTTGACATCT ACCAGACTAG AGTGTTCCCT 
TCCAAACAAT TTGATGTCCT TAAGGATGAC TATGACTATG TCTTAATCAG CAGTGTCCTC 
CTCGGTCTCT TCTTTGCCAC CATGATCAGC AAACGTCTAG CGGAAGTGAA ACTGCTCAAT 
AGGGCATGGC GATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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EMC1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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