BTAF1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	BTAF1
Entrez Gene ID	565357
Full Name	BTAF1 RNA polymerase II, B-TFIID transcription factor-associated
Synonyms	TAFII,wu:fd60h10,wu:fe26c12
General protein information	Preferred Names BTAF1 RNA polymerase II, B-TFIID transcription factor-associated Names TATA-binding protein-associated factor 172 BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005169532.4	XP_005169589.1	TATA-binding protein-associated factor 172
XM_005169532.3	XP_005169589.1	TATA-binding protein-associated factor 172

Pathways from BioSystems

Homologies

Homo sapiens (human)	BTAF1	NP_003963.1
Danio rerio (zebrafish)	btaf1	XP_005169589.1
Saccharomyces cerevisiae (baker's yeast)	MOT1	NP_015243.1
Gallus gallus (chicken)	BTAF1	XP_421689.3
Eremothecium gossypii	AGOS_AEL256C	NP_984605.2
Magnaporthe oryzae (rice blast fungus)	MGG_04989	XP_003712489.1
Pan troglodytes (chimpanzee)	BTAF1	XP_003312799.1
Canis lupus familiaris (dog)	BTAF1	XP_003640032.1
Bos taurus (cattle)	BTAF1	XP_003587915.2
Arabidopsis thaliana (thale cress)	RGD3	NP_190996.3
Rattus norvegicus (Norway rat)	Btaf1	NP_001178846.1
Drosophila melanogaster (fruit fly)	Hel89B	NP_732097.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001820	XP_321235.5
Schizosaccharomyces pombe (fission yeast)	mot1	NP_596080.2
	BTAF1	XP_002805795.1
Mus musculus (house mouse)	Btaf1	NP_001074175.1
Kluyveromyces lactis	KLLA0E23717g	XP_455023.1
Neurospora crassa	NCU07556	XP_958601.1
Xenopus tropicalis (tropical clawed frog)	btaf1	XP_002939139.2

Gene Ontology

The following BTAF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BTAF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa19080	XM_005169532.4 Latest version!	Danio rerio BTAF1 RNA polymerase II, B-TFIID transcription factor-associated (btaf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa19080	XM_005169532.3	Danio rerio BTAF1 RNA polymerase II, B-TFIID transcription factor-associated (btaf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BTAF1 ( XM_005169532.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005169589.1 Danio rerio BTAF1 RNA polymerase II, B-TFIID transcription factor-associated (btaf1), mRNA.

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CloneID	ODa19080
Clone ID Related Accession (Same CDS sequence)	XM_005169532.4 , XM_005169532.3
Accession Version	XM_005169532.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	TATA-binding protein-associated factor 172
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169532.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGTTT CAAGGTTAGA ACGTCTCTTT ATCTTATTGG ATACAGGAAC GACTCCTGTC 
ACCCGTAAAG CTGCTGCTCA GCAGTTGGGA GATGTTGTTA AGCTTCACCC ACATGAGCTT 
AATAACCTGC TATCAAAGGT TTTAACATAC TTAAGAAGTC CAAACTGGGA TACGCGAATT 
GCTGCTGGAC AAGCTGTCGA AGCCATAGTG AAGAATATTC CAGAATGGAA CCCATCTCCA 
AAGCCAAAAG ATGAGGTCTG TGCAGAAGAT TTGTCTCCAG AGGATGTGTC CACAGACAGG 
CTGAGTTTCT ACAGGTTTGA TATCTCTCGT CTGCTGAAGC ACGGAGCATC TCTGCTCGGC 
TCGGCTGGTG CTGAGTTTGA GCTTCAAGAT GACAAGTCAG GTGAGACAGA TCCCAAAGAG 
CGCTTGGCCC GGCAGAGGAA GCAGTTACAG AAGAAGCTGG GATTGGATAT GGGTGCGGCG 
ATCGGCATGG ACACAGAGGA GCTTTTTAAT GATGAGGACC TGGAGGACGC TTGCGCCTCC 
AGTACCAACA GAACACAACC TGGAAAAAGC CTGGGCTGTC ACTTCTCACG CAATCATTTG 
CCAGCTGCAG AGCTCATAGA TTCTGAGTTT CGTCCAGGCA TGAGCAATCG ACAGAAGAAC 
AAAGCAAAGA GGATGGCAAA ACTGGTGGCA AAGCAGAGAT CCAGAGACGT GGATTCTAAT 
GAGAAGAGCA ATGACAGTTT TGAGGGAGAG CCTGAAGAGA AGCGACGGAA AACAACAAAT 
GTAGTCATTG AGCAGCCAGC GAACGACAGC AAAGTCTTAA TTGACAACGT TCCTGATAAC 
TCCAGTTTAT TTGAGGAGAC TCAGGAATGG CCTCTGGAAA GCTTTTGTGA TGAACTGTGC 
AATGACCTCT TCAATCCCTC GTGGGAGATT CGTCATGGTG CTGGCACAGG ACTGAGGGAA 
GTGCTGAAGT GTCATGGTGC TGGCGGAGGA AAGACTGTTG GCAGCACAGC AGAACAGATG 
GAGCGTCAAC ATCAGGAGTG GCTGGAGGAT TTGGTCATCA GACTCTTGTG CGTCTTTGCT 
TTAGACAGAT TTGGAGATTT TGTATCGGAT GAGGTGGTTG CACCTGTCAG AGAGACATGT 
GCTCAGACTC TGGGTGTAGC TTTAAGGCAC ATGGAAAACA GTGGCGTTGC AATGACTGTA 
GACATTCTGC TCAAGCTGCT AACCGAAGAC CAGTGGGAAG TTCGACACGG CGGTCTGTTG 
GGCATCAAAT ATGCTCTGGC TGTCAGACAG GATTTGATCG CAGAGCTGCT TCCTCGAGTT 
CTTCCTGCCA TTACAGAAGG TCTGCGGGAT TTGGATGATG ACGTTCGTGC TGTGGCAGCA 
GCGGCTTTGA TCCCAGTGGT AGATGGATTG GTTCAGTTAC AGCCTGCTAA GGTGCCCTTC 
ATCGTGGACA CACTGTGGAA TGCTCTGCTG GAGCTGGATG ATCTCACAGC CTCGACTAAC 
AGCATCATGA CCCTCCTGTC CTCCCTGCTC ACCTACCCAC AGGTCCGACA GTGCAGCACA 
CAACAGTCTC TGACAGTGCT GGTTCCTAGA GTGTGGCCCT TCTTGAGACA TACCATCGCT 
TCTGTTCGTC GTGCTGCACT AGATACCCTC TTTACACTGC TTTCCAAAGC TGACCAGAGT 
TGTGCAGTGT GGCTGAATCC CATCATGCAG GACATGTTGA GACACATCTT TCAGTCCTGC 
ATCCTGGAGA GCAACCAGGA GATACTAGAG CTGATACAGA AGGTGTGGAG AGAGTTGTTG 
CGTCAGGCCC CGCAGCAGTA TGTGGTGGCG GCCAGCTGTC CCTGGATGGG TGCGTGGCTC 
TGTTTGATGA TGCAAGCGCC ACACATTCCA ATAGACCCCA ACATGCTGCT GGAAGTGAAA 
GCCCGCTTTA AGGAGAAAGC TGCAGGAAAG GCCCGACAGG GATCTGTTCC AGTCAAGGAA 
ACAGTCCAGG AATACATTGG CGGAGCCGAG ACCGTCACAG AAGAGCCTGC CACGCGAGAC 
TATGTGGTGA CACGTGCACG TCTAATGGCT GCGAAGCTGT TGGGGGCGCT GTGCAGTTGT 
ATATGTGACC CACGGTTGAA CAGCTCTTCC CTAGAGCTCC GTCCTGCAGA GTCTCTCGCT 
CAACTTTTAC TCTTCCACCT CAATTCCAAA TCAGCCCTGC AGCGGATTGC AGTTTCCATG 
GTCATCTGTG AGTGGGCCGG CCTTCAGAAG GAATGTGAGC TGCTTTCTAG TGTGGTTCAG 
CCTCGACTGC TGGTCATCCT GTCAGAGCAG CTCTACTATG ACGAGATCGC CATTCCTTTC 
ACTCGTATGC AGAACGAATG CAAACAGCTC ATAGCGCTAC TGGCTGATGC CCACATTGAC 
GTGAGAGAAC GAATCAACTC CACTGTCTTC ACCATCGATC AGGCCAATGA GCTGGTAACC 
ACCATCTTCT CTGAAAGCAC CTCCACTCTG AACCTAAAGT CTCGTCAGTT CCAGCCACTA 
GACAGCAAGC GAATTCAGGC TCAGTCCACA GTGTGTGAGA CCAGCGCCGA ATGGCAGCAG 
CTGCAGCTCA GAGTGCACAC CTTCACTGCC TGCGCTGTGG TGGGCCTGGG GATGTTGCCG 
GATAAACTCA ATCCTGTGGT GCGGCCCCTG ATGGAGGCGG CCCGGCGGGA GGAAAACAGC 
CTCGTCCAGG GTTGTGCGGC ATCCAACATC GCACATCTGC TGCAGCTGTG TGCTGCCAGA 
TCTCCGTGTC CTAATGCAAA GATTGTGAAG AATCTCTGCA GCTCAGTTTG TGTGGACCCC 
ATGCTCACGC CTAGTGCTGC ATGTCCCGTC CCGCCAGCTA GCACTCCAAT ACAGGAGAGC 
AGTAAAGCTT CAGTGGCAGA GAAGGATGCA ATGCATCACA TGGTGAACAA AACAAAGGGC 
ATCATCACTC TGTACCGGCA TCAGAAAGCA GCGTTTGCCA TCACCAGTAA AAGAGGACCG 
ACCCCCAAAA CCCCTAAAAG CAACACTAAC AATGACCTGC CCGTCGGGGG CAGCATTAGC 
ACAGAAACAG ATGAGAGCAA GAAGCCGTGT CTCATCCAGA GGAGAGGGGC AGAGTTCTGT 
CTGATGACTG TTGCCAGACA TTTCAGCAAA GAGTTGACAA AAACTCTGCC CTATCTGTGG 
GAGTCTATGA CCGGCCCATT GAGGAATGCA CTGGATGCTC AGGAATTTGA TTCCAGCCAG 
TTGTTGAAGC AGGGCGACTC TGTGGCTCAG GATCTTGTAA ACTCTCTCCA AGTGCTTGAA 
GTCACAGCAG GAGCCATGTC ACAAGAGCTC ATCCCTTTAT TGATGGAGCA GCTGCCTCTG 
CTGTGCACCT GTCTGCAGCA TCCATATACC GCTGTACGGC ATATGGCCGC TCGCTGTGTT 
GGCGTTCTCA GCAAGATCGC CACAATGGAG ACCATGAATG TGTTTCTGGA GCAGGTGCTG 
CCCTGGCTGG GTGCTATTGA TGACAACACC AAGCAGGAGG GAGCCATCGA AGCCCTGGCC 
TGTGTAATGG AGCAGTTGGA TGTTGATATT GTGCCCTACA TCGTGCTGCT GGTGGTCCCA 
GTGTTGGGTC GTATGAGTGA CCCCTGTGAC AGCGTGCGCT TCATGGCCAC ACAGTGCTTT 
GCTACTCTGA TTCGACTGCT TCCTCTCGAG GCTGGAATCC CAGACCCACC ATCGATGTCT 
GAAGATCTGA TTCGGCAGAA GGCCAGAGAA CGACACTTTC TTGAGCAGCT GCTGGATGGA 
AGAAAACTGG AAAATTACAA GATTCCTGTA CCCATCAAAG CAGAGCTCAG GAAATACCAG 
CAGGATGGTG TGAACTGGCT GGCGTTTTTG AACAAGTACA AGCTCCATGG GATTTTGTGT 
GATGACATGG GTCTGGGTAA AACCCTGCAG TCCATCTGTA TTCTGGCTGG TGATCATTTC 
CTCAGAGCTC AGGAATACAC CAGGACAAAA GCCCCCGACT CTTGCCCTTT GCCCTCCATA 
GTGGTGTGTC CGCCCACCCT GACGGGCCAC TGGGTGGATG AGGTCGGCAA GTTCTGCTCT 
AAAGAGTTTC TTAATCCTCT GCATTATACT GGCCCTCCCA CTGAACGAGC ACGGTTGCAG 
CACCAGGTGA AAAAACACAA TCTTATTGTT GCCTCATATG ATGTCGTTAG GAATGATGTC 
GAATTTTTCA GGGATATTAA ATTTAATTAC TGTATCCTTG ACGAGGGTCA CGTTATTAAG 
AACGGAAAGA CCAAACTCTC CAAAGCGATA AAGCAGTTAA CTGCCAACTA CAGATTGATT 
CTCTCAGGAA CGCCCATCCA GAACAATGTT CTGGAGTTGT GGTCTCTTTT TGACTTCCTG 
ATGCCAGGCT TTCTTGGTAC CGAGCGTCAG TTTGCTGCTC GCTATGGGAA GCCCATCCTC 
GCCAGTCGAG ATGCTAAAAG TTCCTCACGT GAACAGGAAG CAGGCGTGCT GGCAATGGAG 
GCATTGCATA GACAAGTTCT GCCCTTCTTA CTGAGGAGAA TGAAGGATGA TGTGCTTCAA 
GATCTGCCAC CCAAGATCAT ACAGGATTAC TACTGCAACC TCAGTCCTCT ACAGGTTCAA 
CTGTATGAAG ACTTTGCAAA ATCTCGCGCG AAGGTGAACG TGGATGACGT GATTTCTACA 
GCGTCTACAC AAGAGGATGA AGAGAAGCCC AAACTCAAGG CCACTGGACA CGTCTTCCAG 
GCTCTGCAGT ATCTGAGGAA GCTGTGTAAT CATCCTGCAC TGGTTCTGAC TCCTCAACAC 
CCCGAATATA AGCACATCAC AGAGCAGCTG AGCACTCAGC ACTCCAGTCT CAGAGACATC 
CAGCACGCTC CCAAACTCTC AGCCCTCAAA CAGCTGCTGT TGGACTGTGG TCTGGGTTCT 
GCCGGGGCAT CTGACAGCGG CACAGAAGCA GTCGTGGCTC AGCACCGTGT GCTCATCTTC 
TGTCAGCTGA AGAGCATGCT GGACATTGTG GAGCAGGACC TCCTCAAACC ACAGCTGCCC 
GGCGTCACAT ACCTCAGGCT GGACGGTAGC GTTCAGGCCG GACTCAGACA CTCCATAGTC 
TCCAGGTTCA ATAATGACCC GTCCATAGAC GTTCTGCTGC TTACTACACA TGTGGGTGGA 
CTTGGCTTAA ATCTGACTGG TGCGGATACT GTGGTGTTTG TGGAGCACGA CTGGAACCCC 
ATGAGGGACC TGCAGGCCAT GGACAGAGCT CACAGAATAG GACAGAAACG TGTGGTGAAT 
GTATATCGTT TGATCACACG AGGCACACTG GAGGAAAAGA TTATGGGTCT GCAGAAATTC 
AAGATGACCA TCGCCAATAC AGTGATCAGC CAGGAGAACG CCAGCCTGCA GAGCATGGGC 
ACAGAGCAAC TGCTCAACCT CTTCACACTG GACAAGGATG ATAAAGCAGA AAAGTGTGAT 
GGTTCCTCCT CATCCTCCGG TAAATCCAGT ATGAAGTCAG TTCTGGATGG ACTGGGTGAC 
TTGTGGGACC AGCAGCAGTA CGAGAATGAG TATGACCTGG GCACCTTCAT GCACTCGCTC 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169589.1
CDS	323..5908
Translation	MAVSRLERLFILLDTGTTPVTRKAAAQQLGDVVKLHPHELNNLLSKVLTYLRSPNWDTRIAAGQAVEAIVKNIPEWNPSPKPKDEVCAEDLSPEDVSTDRLSFYRFDISRLLKHGASLLGSAGAEFELQDDKSGETDPKERLARQRKQLQKKLGLDMGAAIGMDTEELFNDEDLEDACASSTNRTQPGKSLGCHFSRNHLPAAELIDSEFRPGMSNRQKNKAKRMAKLVAKQRSRDVDSNEKSNDSFEGEPEEKRRKTTNVVIEQPANDSKVLIDNVPDNSSLFEETQEWPLESFCDELCNDLFNPSWEIRHGAGTGLREVLKCHGAGGGKTVGSTAEQMERQHQEWLEDLVIRLLCVFALDRFGDFVSDEVVAPVRETCAQTLGVALRHMENSGVAMTVDILLKLLTEDQWEVRHGGLLGIKYALAVRQDLIAELLPRVLPAITEGLRDLDDDVRAVAAAALIPVVDGLVQLQPAKVPFIVDTLWNALLELDDLTASTNSIMTLLSSLLTYPQVRQCSTQQSLTVLVPRVWPFLRHTIASVRRAALDTLFTLLSKADQSCAVWLNPIMQDMLRHIFQSCILESNQEILELIQKVWRELLRQAPQQYVVAASCPWMGAWLCLMMQAPHIPIDPNMLLEVKARFKEKAAGKARQGSVPVKETVQEYIGGAETVTEEPATRDYVVTRARLMAAKLLGALCSCICDPRLNSSSLELRPAESLAQLLLFHLNSKSALQRIAVSMVICEWAGLQKECELLSSVVQPRLLVILSEQLYYDEIAIPFTRMQNECKQLIALLADAHIDVRERINSTVFTIDQANELVTTIFSESTSTLNLKSRQFQPLDSKRIQAQSTVCETSAEWQQLQLRVHTFTACAVVGLGMLPDKLNPVVRPLMEAARREENSLVQGCAASNIAHLLQLCAARSPCPNAKIVKNLCSSVCVDPMLTPSAACPVPPASTPIQESSKASVAEKDAMHHMVNKTKGIITLYRHQKAAFAITSKRGPTPKTPKSNTNNDLPVGGSISTETDESKKPCLIQRRGAEFCLMTVARHFSKELTKTLPYLWESMTGPLRNALDAQEFDSSQLLKQGDSVAQDLVNSLQVLEVTAGAMSQELIPLLMEQLPLLCTCLQHPYTAVRHMAARCVGVLSKIATMETMNVFLEQVLPWLGAIDDNTKQEGAIEALACVMEQLDVDIVPYIVLLVVPVLGRMSDPCDSVRFMATQCFATLIRLLPLEAGIPDPPSMSEDLIRQKARERHFLEQLLDGRKLENYKIPVPIKAELRKYQQDGVNWLAFLNKYKLHGILCDDMGLGKTLQSICILAGDHFLRAQEYTRTKAPDSCPLPSIVVCPPTLTGHWVDEVGKFCSKEFLNPLHYTGPPTERARLQHQVKKHNLIVASYDVVRNDVEFFRDIKFNYCILDEGHVIKNGKTKLSKAIKQLTANYRLILSGTPIQNNVLELWSLFDFLMPGFLGTERQFAARYGKPILASRDAKSSSREQEAGVLAMEALHRQVLPFLLRRMKDDVLQDLPPKIIQDYYCNLSPLQVQLYEDFAKSRAKVNVDDVISTASTQEDEEKPKLKATGHVFQALQYLRKLCNHPALVLTPQHPEYKHITEQLSTQHSSLRDIQHAPKLSALKQLLLDCGLGSAGASDSGTEAVVAQHRVLIFCQLKSMLDIVEQDLLKPQLPGVTYLRLDGSVQAGLRHSIVSRFNNDPSIDVLLLTTHVGGLGLNLTGADTVVFVEHDWNPMRDLQAMDRAHRIGQKRVVNVYRLITRGTLEEKIMGLQKFKMTIANTVISQENASLQSMGTEQLLNLFTLDKDDKAEKCDGSSSSSGKSSMKSVLDGLGDLWDQQQYENEYDLGTFMHSLK

Target ORF information:

RefSeq Version	XM_005169532.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio BTAF1 RNA polymerase II, B-TFIID transcription factor-associated (btaf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169532.4

ORF Insert Sequence:

ATGGCCGTTT CAAGGTTAGA ACGTCTCTTT ATCTTATTGG ATACAGGAAC GACTCCTGTC 
ACCCGTAAAG CTGCTGCTCA GCAGTTGGGA GATGTTGTTA AGCTTCACCC ACATGAGCTT 
AATAACCTGC TATCAAAGGT TTTAACATAC TTAAGAAGTC CAAACTGGGA TACGCGAATT 
GCTGCTGGAC AAGCTGTCGA AGCCATAGTG AAGAATATTC CAGAATGGAA CCCATCTCCA 
AAGCCAAAAG ATGAGGTCTG TGCAGAAGAT TTGTCTCCAG AGGATGTGTC CACAGACAGG 
CTGAGTTTCT ACAGGTTTGA TATCTCTCGT CTGCTGAAGC ACGGAGCATC TCTGCTCGGC 
TCGGCTGGTG CTGAGTTTGA GCTTCAAGAT GACAAGTCAG GTGAGACAGA TCCCAAAGAG 
CGCTTGGCCC GGCAGAGGAA GCAGTTACAG AAGAAGCTGG GATTGGATAT GGGTGCGGCG 
ATCGGCATGG ACACAGAGGA GCTTTTTAAT GATGAGGACC TGGAGGACGC TTGCGCCTCC 
AGTACCAACA GAACACAACC TGGAAAAAGC CTGGGCTGTC ACTTCTCACG CAATCATTTG 
CCAGCTGCAG AGCTCATAGA TTCTGAGTTT CGTCCAGGCA TGAGCAATCG ACAGAAGAAC 
AAAGCAAAGA GGATGGCAAA ACTGGTGGCA AAGCAGAGAT CCAGAGACGT GGATTCTAAT 
GAGAAGAGCA ATGACAGTTT TGAGGGAGAG CCTGAAGAGA AGCGACGGAA AACAACAAAT 
GTAGTCATTG AGCAGCCAGC GAACGACAGC AAAGTCTTAA TTGACAACGT TCCTGATAAC 
TCCAGTTTAT TTGAGGAGAC TCAGGAATGG CCTCTGGAAA GCTTTTGTGA TGAACTGTGC 
AATGACCTCT TCAATCCCTC GTGGGAGATT CGTCATGGTG CTGGCACAGG ACTGAGGGAA 
GTGCTGAAGT GTCATGGTGC TGGCGGAGGA AAGACTGTTG GCAGCACAGC AGAACAGATG 
GAGCGTCAAC ATCAGGAGTG GCTGGAGGAT TTGGTCATCA GACTCTTGTG CGTCTTTGCT 
TTAGACAGAT TTGGAGATTT TGTATCGGAT GAGGTGGTTG CACCTGTCAG AGAGACATGT 
GCTCAGACTC TGGGTGTAGC TTTAAGGCAC ATGGAAAACA GTGGCGTTGC AATGACTGTA 
GACATTCTGC TCAAGCTGCT AACCGAAGAC CAGTGGGAAG TTCGACACGG CGGTCTGTTG 
GGCATCAAAT ATGCTCTGGC TGTCAGACAG GATTTGATCG CAGAGCTGCT TCCTCGAGTT 
CTTCCTGCCA TTACAGAAGG TCTGCGGGAT TTGGATGATG ACGTTCGTGC TGTGGCAGCA 
GCGGCTTTGA TCCCAGTGGT AGATGGATTG GTTCAGTTAC AGCCTGCTAA GGTGCCCTTC 
ATCGTGGACA CACTGTGGAA TGCTCTGCTG GAGCTGGATG ATCTCACAGC CTCGACTAAC 
AGCATCATGA CCCTCCTGTC CTCCCTGCTC ACCTACCCAC AGGTCCGACA GTGCAGCACA 
CAACAGTCTC TGACAGTGCT GGTTCCTAGA GTGTGGCCCT TCTTGAGACA TACCATCGCT 
TCTGTTCGTC GTGCTGCACT AGATACCCTC TTTACACTGC TTTCCAAAGC TGACCAGAGT 
TGTGCAGTGT GGCTGAATCC CATCATGCAG GACATGTTGA GACACATCTT TCAGTCCTGC 
ATCCTGGAGA GCAACCAGGA GATACTAGAG CTGATACAGA AGGTGTGGAG AGAGTTGTTG 
CGTCAGGCCC CGCAGCAGTA TGTGGTGGCG GCCAGCTGTC CCTGGATGGG TGCGTGGCTC 
TGTTTGATGA TGCAAGCGCC ACACATTCCA ATAGACCCCA ACATGCTGCT GGAAGTGAAA 
GCCCGCTTTA AGGAGAAAGC TGCAGGAAAG GCCCGACAGG GATCTGTTCC AGTCAAGGAA 
ACAGTCCAGG AATACATTGG CGGAGCCGAG ACCGTCACAG AAGAGCCTGC CACGCGAGAC 
TATGTGGTGA CACGTGCACG TCTAATGGCT GCGAAGCTGT TGGGGGCGCT GTGCAGTTGT 
ATATGTGACC CACGGTTGAA CAGCTCTTCC CTAGAGCTCC GTCCTGCAGA GTCTCTCGCT 
CAACTTTTAC TCTTCCACCT CAATTCCAAA TCAGCCCTGC AGCGGATTGC AGTTTCCATG 
GTCATCTGTG AGTGGGCCGG CCTTCAGAAG GAATGTGAGC TGCTTTCTAG TGTGGTTCAG 
CCTCGACTGC TGGTCATCCT GTCAGAGCAG CTCTACTATG ACGAGATCGC CATTCCTTTC 
ACTCGTATGC AGAACGAATG CAAACAGCTC ATAGCGCTAC TGGCTGATGC CCACATTGAC 
GTGAGAGAAC GAATCAACTC CACTGTCTTC ACCATCGATC AGGCCAATGA GCTGGTAACC 
ACCATCTTCT CTGAAAGCAC CTCCACTCTG AACCTAAAGT CTCGTCAGTT CCAGCCACTA 
GACAGCAAGC GAATTCAGGC TCAGTCCACA GTGTGTGAGA CCAGCGCCGA ATGGCAGCAG 
CTGCAGCTCA GAGTGCACAC CTTCACTGCC TGCGCTGTGG TGGGCCTGGG GATGTTGCCG 
GATAAACTCA ATCCTGTGGT GCGGCCCCTG ATGGAGGCGG CCCGGCGGGA GGAAAACAGC 
CTCGTCCAGG GTTGTGCGGC ATCCAACATC GCACATCTGC TGCAGCTGTG TGCTGCCAGA 
TCTCCGTGTC CTAATGCAAA GATTGTGAAG AATCTCTGCA GCTCAGTTTG TGTGGACCCC 
ATGCTCACGC CTAGTGCTGC ATGTCCCGTC CCGCCAGCTA GCACTCCAAT ACAGGAGAGC 
AGTAAAGCTT CAGTGGCAGA GAAGGATGCA ATGCATCACA TGGTGAACAA AACAAAGGGC 
ATCATCACTC TGTACCGGCA TCAGAAAGCA GCGTTTGCCA TCACCAGTAA AAGAGGACCG 
ACCCCCAAAA CCCCTAAAAG CAACACTAAC AATGACCTGC CCGTCGGGGG CAGCATTAGC 
ACAGAAACAG ATGAGAGCAA GAAGCCGTGT CTCATCCAGA GGAGAGGGGC AGAGTTCTGT 
CTGATGACTG TTGCCAGACA TTTCAGCAAA GAGTTGACAA AAACTCTGCC CTATCTGTGG 
GAGTCTATGA CCGGCCCATT GAGGAATGCA CTGGATGCTC AGGAATTTGA TTCCAGCCAG 
TTGTTGAAGC AGGGCGACTC TGTGGCTCAG GATCTTGTAA ACTCTCTCCA AGTGCTTGAA 
GTCACAGCAG GAGCCATGTC ACAAGAGCTC ATCCCTTTAT TGATGGAGCA GCTGCCTCTG 
CTGTGCACCT GTCTGCAGCA TCCATATACC GCTGTACGGC ATATGGCCGC TCGCTGTGTT 
GGCGTTCTCA GCAAGATCGC CACAATGGAG ACCATGAATG TGTTTCTGGA GCAGGTGCTG 
CCCTGGCTGG GTGCTATTGA TGACAACACC AAGCAGGAGG GAGCCATCGA AGCCCTGGCC 
TGTGTAATGG AGCAGTTGGA TGTTGATATT GTGCCCTACA TCGTGCTGCT GGTGGTCCCA 
GTGTTGGGTC GTATGAGTGA CCCCTGTGAC AGCGTGCGCT TCATGGCCAC ACAGTGCTTT 
GCTACTCTGA TTCGACTGCT TCCTCTCGAG GCTGGAATCC CAGACCCACC ATCGATGTCT 
GAAGATCTGA TTCGGCAGAA GGCCAGAGAA CGACACTTTC TTGAGCAGCT GCTGGATGGA 
AGAAAACTGG AAAATTACAA GATTCCTGTA CCCATCAAAG CAGAGCTCAG GAAATACCAG 
CAGGATGGTG TGAACTGGCT GGCGTTTTTG AACAAGTACA AGCTCCATGG GATTTTGTGT 
GATGACATGG GTCTGGGTAA AACCCTGCAG TCCATCTGTA TTCTGGCTGG TGATCATTTC 
CTCAGAGCTC AGGAATACAC CAGGACAAAA GCCCCCGACT CTTGCCCTTT GCCCTCCATA 
GTGGTGTGTC CGCCCACCCT GACGGGCCAC TGGGTGGATG AGGTCGGCAA GTTCTGCTCT 
AAAGAGTTTC TTAATCCTCT GCATTATACT GGCCCTCCCA CTGAACGAGC ACGGTTGCAG 
CACCAGGTGA AAAAACACAA TCTTATTGTT GCCTCATATG ATGTCGTTAG GAATGATGTC 
GAATTTTTCA GGGATATTAA ATTTAATTAC TGTATCCTTG ACGAGGGTCA CGTTATTAAG 
AACGGAAAGA CCAAACTCTC CAAAGCGATA AAGCAGTTAA CTGCCAACTA CAGATTGATT 
CTCTCAGGAA CGCCCATCCA GAACAATGTT CTGGAGTTGT GGTCTCTTTT TGACTTCCTG 
ATGCCAGGCT TTCTTGGTAC CGAGCGTCAG TTTGCTGCTC GCTATGGGAA GCCCATCCTC 
GCCAGTCGAG ATGCTAAAAG TTCCTCACGT GAACAGGAAG CAGGCGTGCT GGCAATGGAG 
GCATTGCATA GACAAGTTCT GCCCTTCTTA CTGAGGAGAA TGAAGGATGA TGTGCTTCAA 
GATCTGCCAC CCAAGATCAT ACAGGATTAC TACTGCAACC TCAGTCCTCT ACAGGTTCAA 
CTGTATGAAG ACTTTGCAAA ATCTCGCGCG AAGGTGAACG TGGATGACGT GATTTCTACA 
GCGTCTACAC AAGAGGATGA AGAGAAGCCC AAACTCAAGG CCACTGGACA CGTCTTCCAG 
GCTCTGCAGT ATCTGAGGAA GCTGTGTAAT CATCCTGCAC TGGTTCTGAC TCCTCAACAC 
CCCGAATATA AGCACATCAC AGAGCAGCTG AGCACTCAGC ACTCCAGTCT CAGAGACATC 
CAGCACGCTC CCAAACTCTC AGCCCTCAAA CAGCTGCTGT TGGACTGTGG TCTGGGTTCT 
GCCGGGGCAT CTGACAGCGG CACAGAAGCA GTCGTGGCTC AGCACCGTGT GCTCATCTTC 
TGTCAGCTGA AGAGCATGCT GGACATTGTG GAGCAGGACC TCCTCAAACC ACAGCTGCCC 
GGCGTCACAT ACCTCAGGCT GGACGGTAGC GTTCAGGCCG GACTCAGACA CTCCATAGTC 
TCCAGGTTCA ATAATGACCC GTCCATAGAC GTTCTGCTGC TTACTACACA TGTGGGTGGA 
CTTGGCTTAA ATCTGACTGG TGCGGATACT GTGGTGTTTG TGGAGCACGA CTGGAACCCC 
ATGAGGGACC TGCAGGCCAT GGACAGAGCT CACAGAATAG GACAGAAACG TGTGGTGAAT 
GTATATCGTT TGATCACACG AGGCACACTG GAGGAAAAGA TTATGGGTCT GCAGAAATTC 
AAGATGACCA TCGCCAATAC AGTGATCAGC CAGGAGAACG CCAGCCTGCA GAGCATGGGC 
ACAGAGCAAC TGCTCAACCT CTTCACACTG GACAAGGATG ATAAAGCAGA AAAGTGTGAT 
GGTTCCTCCT CATCCTCCGG TAAATCCAGT ATGAAGTCAG TTCTGGATGG ACTGGGTGAC 
TTGTGGGACC AGCAGCAGTA CGAGAATGAG TATGACCTGG GCACCTTCAT GCACTCGCTC 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BTAF1 ( XM_005169532.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005169589.1 Danio rerio BTAF1 RNA polymerase II, B-TFIID transcription factor-associated (btaf1), mRNA.

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CloneID	ODa19080
Clone ID Related Accession (Same CDS sequence)	XM_005169532.4 , XM_005169532.3
Accession Version	XM_005169532.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	TATA-binding protein-associated factor 172
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169532.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGTTT CAAGGTTAGA ACGTCTCTTT ATCTTATTGG ATACAGGAAC GACTCCTGTC 
ACCCGTAAAG CTGCTGCTCA GCAGTTGGGA GATGTTGTTA AGCTTCACCC ACATGAGCTT 
AATAACCTGC TATCAAAGGT TTTAACATAC TTAAGAAGTC CAAACTGGGA TACGCGAATT 
GCTGCTGGAC AAGCTGTCGA AGCCATAGTG AAGAATATTC CAGAATGGAA CCCATCTCCA 
AAGCCAAAAG ATGAGGTCTG TGCAGAAGAT TTGTCTCCAG AGGATGTGTC CACAGACAGG 
CTGAGTTTCT ACAGGTTTGA TATCTCTCGT CTGCTGAAGC ACGGAGCATC TCTGCTCGGC 
TCGGCTGGTG CTGAGTTTGA GCTTCAAGAT GACAAGTCAG GTGAGACAGA TCCCAAAGAG 
CGCTTGGCCC GGCAGAGGAA GCAGTTACAG AAGAAGCTGG GATTGGATAT GGGTGCGGCG 
ATCGGCATGG ACACAGAGGA GCTTTTTAAT GATGAGGACC TGGAGGACGC TTGCGCCTCC 
AGTACCAACA GAACACAACC TGGAAAAAGC CTGGGCTGTC ACTTCTCACG CAATCATTTG 
CCAGCTGCAG AGCTCATAGA TTCTGAGTTT CGTCCAGGCA TGAGCAATCG ACAGAAGAAC 
AAAGCAAAGA GGATGGCAAA ACTGGTGGCA AAGCAGAGAT CCAGAGACGT GGATTCTAAT 
GAGAAGAGCA ATGACAGTTT TGAGGGAGAG CCTGAAGAGA AGCGACGGAA AACAACAAAT 
GTAGTCATTG AGCAGCCAGC GAACGACAGC AAAGTCTTAA TTGACAACGT TCCTGATAAC 
TCCAGTTTAT TTGAGGAGAC TCAGGAATGG CCTCTGGAAA GCTTTTGTGA TGAACTGTGC 
AATGACCTCT TCAATCCCTC GTGGGAGATT CGTCATGGTG CTGGCACAGG ACTGAGGGAA 
GTGCTGAAGT GTCATGGTGC TGGCGGAGGA AAGACTGTTG GCAGCACAGC AGAACAGATG 
GAGCGTCAAC ATCAGGAGTG GCTGGAGGAT TTGGTCATCA GACTCTTGTG CGTCTTTGCT 
TTAGACAGAT TTGGAGATTT TGTATCGGAT GAGGTGGTTG CACCTGTCAG AGAGACATGT 
GCTCAGACTC TGGGTGTAGC TTTAAGGCAC ATGGAAAACA GTGGCGTTGC AATGACTGTA 
GACATTCTGC TCAAGCTGCT AACCGAAGAC CAGTGGGAAG TTCGACACGG CGGTCTGTTG 
GGCATCAAAT ATGCTCTGGC TGTCAGACAG GATTTGATCG CAGAGCTGCT TCCTCGAGTT 
CTTCCTGCCA TTACAGAAGG TCTGCGGGAT TTGGATGATG ACGTTCGTGC TGTGGCAGCA 
GCGGCTTTGA TCCCAGTGGT AGATGGATTG GTTCAGTTAC AGCCTGCTAA GGTGCCCTTC 
ATCGTGGACA CACTGTGGAA TGCTCTGCTG GAGCTGGATG ATCTCACAGC CTCGACTAAC 
AGCATCATGA CCCTCCTGTC CTCCCTGCTC ACCTACCCAC AGGTCCGACA GTGCAGCACA 
CAACAGTCTC TGACAGTGCT GGTTCCTAGA GTGTGGCCCT TCTTGAGACA TACCATCGCT 
TCTGTTCGTC GTGCTGCACT AGATACCCTC TTTACACTGC TTTCCAAAGC TGACCAGAGT 
TGTGCAGTGT GGCTGAATCC CATCATGCAG GACATGTTGA GACACATCTT TCAGTCCTGC 
ATCCTGGAGA GCAACCAGGA GATACTAGAG CTGATACAGA AGGTGTGGAG AGAGTTGTTG 
CGTCAGGCCC CGCAGCAGTA TGTGGTGGCG GCCAGCTGTC CCTGGATGGG TGCGTGGCTC 
TGTTTGATGA TGCAAGCGCC ACACATTCCA ATAGACCCCA ACATGCTGCT GGAAGTGAAA 
GCCCGCTTTA AGGAGAAAGC TGCAGGAAAG GCCCGACAGG GATCTGTTCC AGTCAAGGAA 
ACAGTCCAGG AATACATTGG CGGAGCCGAG ACCGTCACAG AAGAGCCTGC CACGCGAGAC 
TATGTGGTGA CACGTGCACG TCTAATGGCT GCGAAGCTGT TGGGGGCGCT GTGCAGTTGT 
ATATGTGACC CACGGTTGAA CAGCTCTTCC CTAGAGCTCC GTCCTGCAGA GTCTCTCGCT 
CAACTTTTAC TCTTCCACCT CAATTCCAAA TCAGCCCTGC AGCGGATTGC AGTTTCCATG 
GTCATCTGTG AGTGGGCCGG CCTTCAGAAG GAATGTGAGC TGCTTTCTAG TGTGGTTCAG 
CCTCGACTGC TGGTCATCCT GTCAGAGCAG CTCTACTATG ACGAGATCGC CATTCCTTTC 
ACTCGTATGC AGAACGAATG CAAACAGCTC ATAGCGCTAC TGGCTGATGC CCACATTGAC 
GTGAGAGAAC GAATCAACTC CACTGTCTTC ACCATCGATC AGGCCAATGA GCTGGTAACC 
ACCATCTTCT CTGAAAGCAC CTCCACTCTG AACCTAAAGT CTCGTCAGTT CCAGCCACTA 
GACAGCAAGC GAATTCAGGC TCAGTCCACA GTGTGTGAGA CCAGCGCCGA ATGGCAGCAG 
CTGCAGCTCA GAGTGCACAC CTTCACTGCC TGCGCTGTGG TGGGCCTGGG GATGTTGCCG 
GATAAACTCA ATCCTGTGGT GCGGCCCCTG ATGGAGGCGG CCCGGCGGGA GGAAAACAGC 
CTCGTCCAGG GTTGTGCGGC ATCCAACATC GCACATCTGC TGCAGCTGTG TGCTGCCAGA 
TCTCCGTGTC CTAATGCAAA GATTGTGAAG AATCTCTGCA GCTCAGTTTG TGTGGACCCC 
ATGCTCACGC CTAGTGCTGC ATGTCCCGTC CCGCCAGCTA GCACTCCAAT ACAGGAGAGC 
AGTAAAGCTT CAGTGGCAGA GAAGGATGCA ATGCATCACA TGGTGAACAA AACAAAGGGC 
ATCATCACTC TGTACCGGCA TCAGAAAGCA GCGTTTGCCA TCACCAGTAA AAGAGGACCG 
ACCCCCAAAA CCCCTAAAAG CAACACTAAC AATGACCTGC CCGTCGGGGG CAGCATTAGC 
ACAGAAACAG ATGAGAGCAA GAAGCCGTGT CTCATCCAGA GGAGAGGGGC AGAGTTCTGT 
CTGATGACTG TTGCCAGACA TTTCAGCAAA GAGTTGACAA AAACTCTGCC CTATCTGTGG 
GAGTCTATGA CCGGCCCATT GAGGAATGCA CTGGATGCTC AGGAATTTGA TTCCAGCCAG 
TTGTTGAAGC AGGGCGACTC TGTGGCTCAG GATCTTGTAA ACTCTCTCCA AGTGCTTGAA 
GTCACAGCAG GAGCCATGTC ACAAGAGCTC ATCCCTTTAT TGATGGAGCA GCTGCCTCTG 
CTGTGCACCT GTCTGCAGCA TCCATATACC GCTGTACGGC ATATGGCCGC TCGCTGTGTT 
GGCGTTCTCA GCAAGATCGC CACAATGGAG ACCATGAATG TGTTTCTGGA GCAGGTGCTG 
CCCTGGCTGG GTGCTATTGA TGACAACACC AAGCAGGAGG GAGCCATCGA AGCCCTGGCC 
TGTGTAATGG AGCAGTTGGA TGTTGATATT GTGCCCTACA TCGTGCTGCT GGTGGTCCCA 
GTGTTGGGTC GTATGAGTGA CCCCTGTGAC AGCGTGCGCT TCATGGCCAC ACAGTGCTTT 
GCTACTCTGA TTCGACTGCT TCCTCTCGAG GCTGGAATCC CAGACCCACC ATCGATGTCT 
GAAGATCTGA TTCGGCAGAA GGCCAGAGAA CGACACTTTC TTGAGCAGCT GCTGGATGGA 
AGAAAACTGG AAAATTACAA GATTCCTGTA CCCATCAAAG CAGAGCTCAG GAAATACCAG 
CAGGATGGTG TGAACTGGCT GGCGTTTTTG AACAAGTACA AGCTCCATGG GATTTTGTGT 
GATGACATGG GTCTGGGTAA AACCCTGCAG TCCATCTGTA TTCTGGCTGG TGATCATTTC 
CTCAGAGCTC AGGAATACAC CAGGACAAAA GCCCCCGACT CTTGCCCTTT GCCCTCCATA 
GTGGTGTGTC CGCCCACCCT GACGGGCCAC TGGGTGGATG AGGTCGGCAA GTTCTGCTCT 
AAAGAGTTTC TTAATCCTCT GCATTATACT GGCCCTCCCA CTGAACGAGC ACGGTTGCAG 
CACCAGGTGA AAAAACACAA TCTTATTGTT GCCTCATATG ATGTCGTTAG GAATGATGTC 
GAATTTTTCA GGGATATTAA ATTTAATTAC TGTATCCTTG ACGAGGGTCA CGTTATTAAG 
AACGGAAAGA CCAAACTCTC CAAAGCGATA AAGCAGTTAA CTGCCAACTA CAGATTGATT 
CTCTCAGGAA CGCCCATCCA GAACAATGTT CTGGAGTTGT GGTCTCTTTT TGACTTCCTG 
ATGCCAGGCT TTCTTGGTAC CGAGCGTCAG TTTGCTGCTC GCTATGGGAA GCCCATCCTC 
GCCAGTCGAG ATGCTAAAAG TTCCTCACGT GAACAGGAAG CAGGCGTGCT GGCAATGGAG 
GCATTGCATA GACAAGTTCT GCCCTTCTTA CTGAGGAGAA TGAAGGATGA TGTGCTTCAA 
GATCTGCCAC CCAAGATCAT ACAGGATTAC TACTGCAACC TCAGTCCTCT ACAGGTTCAA 
CTGTATGAAG ACTTTGCAAA ATCTCGCGCG AAGGTGAACG TGGATGACGT GATTTCTACA 
GCGTCTACAC AAGAGGATGA AGAGAAGCCC AAACTCAAGG CCACTGGACA CGTCTTCCAG 
GCTCTGCAGT ATCTGAGGAA GCTGTGTAAT CATCCTGCAC TGGTTCTGAC TCCTCAACAC 
CCCGAATATA AGCACATCAC AGAGCAGCTG AGCACTCAGC ACTCCAGTCT CAGAGACATC 
CAGCACGCTC CCAAACTCTC AGCCCTCAAA CAGCTGCTGT TGGACTGTGG TCTGGGTTCT 
GCCGGGGCAT CTGACAGCGG CACAGAAGCA GTCGTGGCTC AGCACCGTGT GCTCATCTTC 
TGTCAGCTGA AGAGCATGCT GGACATTGTG GAGCAGGACC TCCTCAAACC ACAGCTGCCC 
GGCGTCACAT ACCTCAGGCT GGACGGTAGC GTTCAGGCCG GACTCAGACA CTCCATAGTC 
TCCAGGTTCA ATAATGACCC GTCCATAGAC GTTCTGCTGC TTACTACACA TGTGGGTGGA 
CTTGGCTTAA ATCTGACTGG TGCGGATACT GTGGTGTTTG TGGAGCACGA CTGGAACCCC 
ATGAGGGACC TGCAGGCCAT GGACAGAGCT CACAGAATAG GACAGAAACG TGTGGTGAAT 
GTATATCGTT TGATCACACG AGGCACACTG GAGGAAAAGA TTATGGGTCT GCAGAAATTC 
AAGATGACCA TCGCCAATAC AGTGATCAGC CAGGAGAACG CCAGCCTGCA GAGCATGGGC 
ACAGAGCAAC TGCTCAACCT CTTCACACTG GACAAGGATG ATAAAGCAGA AAAGTGTGAT 
GGTTCCTCCT CATCCTCCGG TAAATCCAGT ATGAAGTCAG TTCTGGATGG ACTGGGTGAC 
TTGTGGGACC AGCAGCAGTA CGAGAATGAG TATGACCTGG GCACCTTCAT GCACTCGCTC 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005169589.1
CDS	320..5905
Translation	MAVSRLERLFILLDTGTTPVTRKAAAQQLGDVVKLHPHELNNLLSKVLTYLRSPNWDTRIAAGQAVEAIVKNIPEWNPSPKPKDEVCAEDLSPEDVSTDRLSFYRFDISRLLKHGASLLGSAGAEFELQDDKSGETDPKERLARQRKQLQKKLGLDMGAAIGMDTEELFNDEDLEDACASSTNRTQPGKSLGCHFSRNHLPAAELIDSEFRPGMSNRQKNKAKRMAKLVAKQRSRDVDSNEKSNDSFEGEPEEKRRKTTNVVIEQPANDSKVLIDNVPDNSSLFEETQEWPLESFCDELCNDLFNPSWEIRHGAGTGLREVLKCHGAGGGKTVGSTAEQMERQHQEWLEDLVIRLLCVFALDRFGDFVSDEVVAPVRETCAQTLGVALRHMENSGVAMTVDILLKLLTEDQWEVRHGGLLGIKYALAVRQDLIAELLPRVLPAITEGLRDLDDDVRAVAAAALIPVVDGLVQLQPAKVPFIVDTLWNALLELDDLTASTNSIMTLLSSLLTYPQVRQCSTQQSLTVLVPRVWPFLRHTIASVRRAALDTLFTLLSKADQSCAVWLNPIMQDMLRHIFQSCILESNQEILELIQKVWRELLRQAPQQYVVAASCPWMGAWLCLMMQAPHIPIDPNMLLEVKARFKEKAAGKARQGSVPVKETVQEYIGGAETVTEEPATRDYVVTRARLMAAKLLGALCSCICDPRLNSSSLELRPAESLAQLLLFHLNSKSALQRIAVSMVICEWAGLQKECELLSSVVQPRLLVILSEQLYYDEIAIPFTRMQNECKQLIALLADAHIDVRERINSTVFTIDQANELVTTIFSESTSTLNLKSRQFQPLDSKRIQAQSTVCETSAEWQQLQLRVHTFTACAVVGLGMLPDKLNPVVRPLMEAARREENSLVQGCAASNIAHLLQLCAARSPCPNAKIVKNLCSSVCVDPMLTPSAACPVPPASTPIQESSKASVAEKDAMHHMVNKTKGIITLYRHQKAAFAITSKRGPTPKTPKSNTNNDLPVGGSISTETDESKKPCLIQRRGAEFCLMTVARHFSKELTKTLPYLWESMTGPLRNALDAQEFDSSQLLKQGDSVAQDLVNSLQVLEVTAGAMSQELIPLLMEQLPLLCTCLQHPYTAVRHMAARCVGVLSKIATMETMNVFLEQVLPWLGAIDDNTKQEGAIEALACVMEQLDVDIVPYIVLLVVPVLGRMSDPCDSVRFMATQCFATLIRLLPLEAGIPDPPSMSEDLIRQKARERHFLEQLLDGRKLENYKIPVPIKAELRKYQQDGVNWLAFLNKYKLHGILCDDMGLGKTLQSICILAGDHFLRAQEYTRTKAPDSCPLPSIVVCPPTLTGHWVDEVGKFCSKEFLNPLHYTGPPTERARLQHQVKKHNLIVASYDVVRNDVEFFRDIKFNYCILDEGHVIKNGKTKLSKAIKQLTANYRLILSGTPIQNNVLELWSLFDFLMPGFLGTERQFAARYGKPILASRDAKSSSREQEAGVLAMEALHRQVLPFLLRRMKDDVLQDLPPKIIQDYYCNLSPLQVQLYEDFAKSRAKVNVDDVISTASTQEDEEKPKLKATGHVFQALQYLRKLCNHPALVLTPQHPEYKHITEQLSTQHSSLRDIQHAPKLSALKQLLLDCGLGSAGASDSGTEAVVAQHRVLIFCQLKSMLDIVEQDLLKPQLPGVTYLRLDGSVQAGLRHSIVSRFNNDPSIDVLLLTTHVGGLGLNLTGADTVVFVEHDWNPMRDLQAMDRAHRIGQKRVVNVYRLITRGTLEEKIMGLQKFKMTIANTVISQENASLQSMGTEQLLNLFTLDKDDKAEKCDGSSSSSGKSSMKSVLDGLGDLWDQQQYENEYDLGTFMHSLK

Target ORF information:

RefSeq Version	XM_005169532.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio BTAF1 RNA polymerase II, B-TFIID transcription factor-associated (btaf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169532.3

ORF Insert Sequence:

ATGGCCGTTT CAAGGTTAGA ACGTCTCTTT ATCTTATTGG ATACAGGAAC GACTCCTGTC 
ACCCGTAAAG CTGCTGCTCA GCAGTTGGGA GATGTTGTTA AGCTTCACCC ACATGAGCTT 
AATAACCTGC TATCAAAGGT TTTAACATAC TTAAGAAGTC CAAACTGGGA TACGCGAATT 
GCTGCTGGAC AAGCTGTCGA AGCCATAGTG AAGAATATTC CAGAATGGAA CCCATCTCCA 
AAGCCAAAAG ATGAGGTCTG TGCAGAAGAT TTGTCTCCAG AGGATGTGTC CACAGACAGG 
CTGAGTTTCT ACAGGTTTGA TATCTCTCGT CTGCTGAAGC ACGGAGCATC TCTGCTCGGC 
TCGGCTGGTG CTGAGTTTGA GCTTCAAGAT GACAAGTCAG GTGAGACAGA TCCCAAAGAG 
CGCTTGGCCC GGCAGAGGAA GCAGTTACAG AAGAAGCTGG GATTGGATAT GGGTGCGGCG 
ATCGGCATGG ACACAGAGGA GCTTTTTAAT GATGAGGACC TGGAGGACGC TTGCGCCTCC 
AGTACCAACA GAACACAACC TGGAAAAAGC CTGGGCTGTC ACTTCTCACG CAATCATTTG 
CCAGCTGCAG AGCTCATAGA TTCTGAGTTT CGTCCAGGCA TGAGCAATCG ACAGAAGAAC 
AAAGCAAAGA GGATGGCAAA ACTGGTGGCA AAGCAGAGAT CCAGAGACGT GGATTCTAAT 
GAGAAGAGCA ATGACAGTTT TGAGGGAGAG CCTGAAGAGA AGCGACGGAA AACAACAAAT 
GTAGTCATTG AGCAGCCAGC GAACGACAGC AAAGTCTTAA TTGACAACGT TCCTGATAAC 
TCCAGTTTAT TTGAGGAGAC TCAGGAATGG CCTCTGGAAA GCTTTTGTGA TGAACTGTGC 
AATGACCTCT TCAATCCCTC GTGGGAGATT CGTCATGGTG CTGGCACAGG ACTGAGGGAA 
GTGCTGAAGT GTCATGGTGC TGGCGGAGGA AAGACTGTTG GCAGCACAGC AGAACAGATG 
GAGCGTCAAC ATCAGGAGTG GCTGGAGGAT TTGGTCATCA GACTCTTGTG CGTCTTTGCT 
TTAGACAGAT TTGGAGATTT TGTATCGGAT GAGGTGGTTG CACCTGTCAG AGAGACATGT 
GCTCAGACTC TGGGTGTAGC TTTAAGGCAC ATGGAAAACA GTGGCGTTGC AATGACTGTA 
GACATTCTGC TCAAGCTGCT AACCGAAGAC CAGTGGGAAG TTCGACACGG CGGTCTGTTG 
GGCATCAAAT ATGCTCTGGC TGTCAGACAG GATTTGATCG CAGAGCTGCT TCCTCGAGTT 
CTTCCTGCCA TTACAGAAGG TCTGCGGGAT TTGGATGATG ACGTTCGTGC TGTGGCAGCA 
GCGGCTTTGA TCCCAGTGGT AGATGGATTG GTTCAGTTAC AGCCTGCTAA GGTGCCCTTC 
ATCGTGGACA CACTGTGGAA TGCTCTGCTG GAGCTGGATG ATCTCACAGC CTCGACTAAC 
AGCATCATGA CCCTCCTGTC CTCCCTGCTC ACCTACCCAC AGGTCCGACA GTGCAGCACA 
CAACAGTCTC TGACAGTGCT GGTTCCTAGA GTGTGGCCCT TCTTGAGACA TACCATCGCT 
TCTGTTCGTC GTGCTGCACT AGATACCCTC TTTACACTGC TTTCCAAAGC TGACCAGAGT 
TGTGCAGTGT GGCTGAATCC CATCATGCAG GACATGTTGA GACACATCTT TCAGTCCTGC 
ATCCTGGAGA GCAACCAGGA GATACTAGAG CTGATACAGA AGGTGTGGAG AGAGTTGTTG 
CGTCAGGCCC CGCAGCAGTA TGTGGTGGCG GCCAGCTGTC CCTGGATGGG TGCGTGGCTC 
TGTTTGATGA TGCAAGCGCC ACACATTCCA ATAGACCCCA ACATGCTGCT GGAAGTGAAA 
GCCCGCTTTA AGGAGAAAGC TGCAGGAAAG GCCCGACAGG GATCTGTTCC AGTCAAGGAA 
ACAGTCCAGG AATACATTGG CGGAGCCGAG ACCGTCACAG AAGAGCCTGC CACGCGAGAC 
TATGTGGTGA CACGTGCACG TCTAATGGCT GCGAAGCTGT TGGGGGCGCT GTGCAGTTGT 
ATATGTGACC CACGGTTGAA CAGCTCTTCC CTAGAGCTCC GTCCTGCAGA GTCTCTCGCT 
CAACTTTTAC TCTTCCACCT CAATTCCAAA TCAGCCCTGC AGCGGATTGC AGTTTCCATG 
GTCATCTGTG AGTGGGCCGG CCTTCAGAAG GAATGTGAGC TGCTTTCTAG TGTGGTTCAG 
CCTCGACTGC TGGTCATCCT GTCAGAGCAG CTCTACTATG ACGAGATCGC CATTCCTTTC 
ACTCGTATGC AGAACGAATG CAAACAGCTC ATAGCGCTAC TGGCTGATGC CCACATTGAC 
GTGAGAGAAC GAATCAACTC CACTGTCTTC ACCATCGATC AGGCCAATGA GCTGGTAACC 
ACCATCTTCT CTGAAAGCAC CTCCACTCTG AACCTAAAGT CTCGTCAGTT CCAGCCACTA 
GACAGCAAGC GAATTCAGGC TCAGTCCACA GTGTGTGAGA CCAGCGCCGA ATGGCAGCAG 
CTGCAGCTCA GAGTGCACAC CTTCACTGCC TGCGCTGTGG TGGGCCTGGG GATGTTGCCG 
GATAAACTCA ATCCTGTGGT GCGGCCCCTG ATGGAGGCGG CCCGGCGGGA GGAAAACAGC 
CTCGTCCAGG GTTGTGCGGC ATCCAACATC GCACATCTGC TGCAGCTGTG TGCTGCCAGA 
TCTCCGTGTC CTAATGCAAA GATTGTGAAG AATCTCTGCA GCTCAGTTTG TGTGGACCCC 
ATGCTCACGC CTAGTGCTGC ATGTCCCGTC CCGCCAGCTA GCACTCCAAT ACAGGAGAGC 
AGTAAAGCTT CAGTGGCAGA GAAGGATGCA ATGCATCACA TGGTGAACAA AACAAAGGGC 
ATCATCACTC TGTACCGGCA TCAGAAAGCA GCGTTTGCCA TCACCAGTAA AAGAGGACCG 
ACCCCCAAAA CCCCTAAAAG CAACACTAAC AATGACCTGC CCGTCGGGGG CAGCATTAGC 
ACAGAAACAG ATGAGAGCAA GAAGCCGTGT CTCATCCAGA GGAGAGGGGC AGAGTTCTGT 
CTGATGACTG TTGCCAGACA TTTCAGCAAA GAGTTGACAA AAACTCTGCC CTATCTGTGG 
GAGTCTATGA CCGGCCCATT GAGGAATGCA CTGGATGCTC AGGAATTTGA TTCCAGCCAG 
TTGTTGAAGC AGGGCGACTC TGTGGCTCAG GATCTTGTAA ACTCTCTCCA AGTGCTTGAA 
GTCACAGCAG GAGCCATGTC ACAAGAGCTC ATCCCTTTAT TGATGGAGCA GCTGCCTCTG 
CTGTGCACCT GTCTGCAGCA TCCATATACC GCTGTACGGC ATATGGCCGC TCGCTGTGTT 
GGCGTTCTCA GCAAGATCGC CACAATGGAG ACCATGAATG TGTTTCTGGA GCAGGTGCTG 
CCCTGGCTGG GTGCTATTGA TGACAACACC AAGCAGGAGG GAGCCATCGA AGCCCTGGCC 
TGTGTAATGG AGCAGTTGGA TGTTGATATT GTGCCCTACA TCGTGCTGCT GGTGGTCCCA 
GTGTTGGGTC GTATGAGTGA CCCCTGTGAC AGCGTGCGCT TCATGGCCAC ACAGTGCTTT 
GCTACTCTGA TTCGACTGCT TCCTCTCGAG GCTGGAATCC CAGACCCACC ATCGATGTCT 
GAAGATCTGA TTCGGCAGAA GGCCAGAGAA CGACACTTTC TTGAGCAGCT GCTGGATGGA 
AGAAAACTGG AAAATTACAA GATTCCTGTA CCCATCAAAG CAGAGCTCAG GAAATACCAG 
CAGGATGGTG TGAACTGGCT GGCGTTTTTG AACAAGTACA AGCTCCATGG GATTTTGTGT 
GATGACATGG GTCTGGGTAA AACCCTGCAG TCCATCTGTA TTCTGGCTGG TGATCATTTC 
CTCAGAGCTC AGGAATACAC CAGGACAAAA GCCCCCGACT CTTGCCCTTT GCCCTCCATA 
GTGGTGTGTC CGCCCACCCT GACGGGCCAC TGGGTGGATG AGGTCGGCAA GTTCTGCTCT 
AAAGAGTTTC TTAATCCTCT GCATTATACT GGCCCTCCCA CTGAACGAGC ACGGTTGCAG 
CACCAGGTGA AAAAACACAA TCTTATTGTT GCCTCATATG ATGTCGTTAG GAATGATGTC 
GAATTTTTCA GGGATATTAA ATTTAATTAC TGTATCCTTG ACGAGGGTCA CGTTATTAAG 
AACGGAAAGA CCAAACTCTC CAAAGCGATA AAGCAGTTAA CTGCCAACTA CAGATTGATT 
CTCTCAGGAA CGCCCATCCA GAACAATGTT CTGGAGTTGT GGTCTCTTTT TGACTTCCTG 
ATGCCAGGCT TTCTTGGTAC CGAGCGTCAG TTTGCTGCTC GCTATGGGAA GCCCATCCTC 
GCCAGTCGAG ATGCTAAAAG TTCCTCACGT GAACAGGAAG CAGGCGTGCT GGCAATGGAG 
GCATTGCATA GACAAGTTCT GCCCTTCTTA CTGAGGAGAA TGAAGGATGA TGTGCTTCAA 
GATCTGCCAC CCAAGATCAT ACAGGATTAC TACTGCAACC TCAGTCCTCT ACAGGTTCAA 
CTGTATGAAG ACTTTGCAAA ATCTCGCGCG AAGGTGAACG TGGATGACGT GATTTCTACA 
GCGTCTACAC AAGAGGATGA AGAGAAGCCC AAACTCAAGG CCACTGGACA CGTCTTCCAG 
GCTCTGCAGT ATCTGAGGAA GCTGTGTAAT CATCCTGCAC TGGTTCTGAC TCCTCAACAC 
CCCGAATATA AGCACATCAC AGAGCAGCTG AGCACTCAGC ACTCCAGTCT CAGAGACATC 
CAGCACGCTC CCAAACTCTC AGCCCTCAAA CAGCTGCTGT TGGACTGTGG TCTGGGTTCT 
GCCGGGGCAT CTGACAGCGG CACAGAAGCA GTCGTGGCTC AGCACCGTGT GCTCATCTTC 
TGTCAGCTGA AGAGCATGCT GGACATTGTG GAGCAGGACC TCCTCAAACC ACAGCTGCCC 
GGCGTCACAT ACCTCAGGCT GGACGGTAGC GTTCAGGCCG GACTCAGACA CTCCATAGTC 
TCCAGGTTCA ATAATGACCC GTCCATAGAC GTTCTGCTGC TTACTACACA TGTGGGTGGA 
CTTGGCTTAA ATCTGACTGG TGCGGATACT GTGGTGTTTG TGGAGCACGA CTGGAACCCC 
ATGAGGGACC TGCAGGCCAT GGACAGAGCT CACAGAATAG GACAGAAACG TGTGGTGAAT 
GTATATCGTT TGATCACACG AGGCACACTG GAGGAAAAGA TTATGGGTCT GCAGAAATTC 
AAGATGACCA TCGCCAATAC AGTGATCAGC CAGGAGAACG CCAGCCTGCA GAGCATGGGC 
ACAGAGCAAC TGCTCAACCT CTTCACACTG GACAAGGATG ATAAAGCAGA AAAGTGTGAT 
GGTTCCTCCT CATCCTCCGG TAAATCCAGT ATGAAGTCAG TTCTGGATGG ACTGGGTGAC 
TTGTGGGACC AGCAGCAGTA CGAGAATGAG TATGACCTGG GCACCTTCAT GCACTCGCTC 
AAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

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BTAF1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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