cel.2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cel.2
Entrez Gene ID	565117
Full Name	carboxyl ester lipase, tandem duplicate 2
Synonyms	celip,zgc:153861
General protein information	Preferred Names carboxyl ester lipase, tandem duplicate 2 Names bile salt-activated lipase carboxyl ester lipase, tandem duplicate 2 danio-celip
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021469026.1	XP_021324701.1	bile salt-activated lipase
XM_009295234.2	XP_009293509.1	bile salt-activated lipase
NM_001025173.2	NP_001020344.2	carboxyl ester lipase, tandem duplicate 2 precursor

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CEL	XP_003951607.1
Gallus gallus (chicken)	CEL	NP_001013015.1
	CEL	XP_001118455.2
Mus musculus (house mouse)	Cel	NP_034015.1
Danio rerio (zebrafish)	cel.2	XP_005161146.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001722	XP_321363.4
Xenopus tropicalis (tropical clawed frog)	LOC100492444	XP_002936238.2
Bos taurus (cattle)	CEL	NP_001013601.1
Homo sapiens (human)	CEL	NP_001798.2
Canis lupus familiaris (dog)	CEL	XP_548401.1
Rattus norvegicus (Norway rat)	Cel	NP_058693.2
Danio rerio (zebrafish)	cel.1	NP_955901.1
Anopheles gambiae (African malaria mosquito)	COEAE1A	XP_321362.2
Anopheles gambiae (African malaria mosquito)	COEAE6O	XP_312052.3
Xenopus tropicalis (tropical clawed frog)	cel	NP_001120027.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The draft genome of the grass carp (Ctenopharyngodon idellus) provides insights into its evolution and vegetarian adaptation.
Wang Y, Lu Y, Zhang Y, Ning Z, Li Y, Zhao Q, Lu H, Huang R, Xia X, Feng Q, Liang X, Liu K, Zhang L, Lu T, Huang T, Fan D, Weng Q, Zhu C, Lu Y, Li W, Wen Z, Zhou C, Tian Q, Kang X, Shi M, Zhang W, Jang S, Du F, He S, Liao L, Li Y, Gui B, He H, Ning Z, Yang C, He L, Luo L, Yang R, Luo Q, Liu X, Li S, Huang W, Xiao L, Lin H, Han B, Zhu Z
Nature genetics47(6)625-31(2015 Jun)

Systems pharmacology of adiposity reveals inhibition of EP300 as a common therapeutic mechanism of caloric restriction and resveratrol for obesity.
Nishimura Y, Sasagawa S, Ariyoshi M, Ichikawa S, Shimada Y, Kawaguchi K, Kawase R, Yamamoto R, Uehara T, Yanai T, Takata R, Tanaka T
Frontiers in pharmacology6199(2015)

Zebrafish acetylcholinesterase is encoded by a single gene localized on linkage group 7. Gene structure and polymorphism; molecular forms and expression pattern during development.
Bertrand C, Chatonnet A, Takke C, Yan YL, Postlethwait J, Toutant JP, Cousin X
The Journal of biological chemistry276(1)464-74(2001 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following cel.2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cel.2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa25663	NM_001025173.2 Latest version!	Danio rerio carboxyl ester lipase, tandem duplicate 2 (cel.2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa25663	XM_021469026.1 Latest version!	Danio rerio carboxyl ester lipase, tandem duplicate 2 (cel.2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa25663	XM_009295234.2 Latest version!	Danio rerio carboxyl ester lipase, tandem duplicate 2 (cel.2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cel.2 ( NM_001025173.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001020344.2 Danio rerio carboxyl ester lipase, tandem duplicate 2 (cel.2), mRNA.

Price & Availability↑

CloneID	ODa25663
Clone ID Related Accession (Same CDS sequence)	XM_021469026.1 , XM_009295234.2 , NM_001025173.2
Accession Version	NM_001025173.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	carboxyl ester lipase, tandem duplicate 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from FP015918.6 and BX005379.9. On or before Mar 28, 2018 this sequence version replaced XM_021469026.1, NM_001025173.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC076049.1, GDQQ01003664.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505371 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGATGA TGCTGGGAGT TTTCATCTCT GCCGCCCTGT TCCTAGGGAC AGCACAAGGA 
GCTTCTTTGG GAGCTGTGCT CACTGAAGGA GGGATGGTGC AGGGAAAGAG TCATAGTGTT 
GGTCTTCTCC GCTACATGGA TGTCTTTAAG GGTATCCCCT TTGCCGCTCC ACCTGGCAGA 
CTGCAGAAGC CGGTTCCTCA CCCTGGCTGG GAAGGTGTCC TGAAAGCCAC TGAATATAGT 
AAGAGGTGTA TTCAGCTCAA TCTCCTTCAA ACGGATACCA TGGGCAGTGA AGACTGTCTG 
TACCTGAACA TATGGGTCCC TCAAGGCAAA ACTGTTTCCT ACAACTTGCC TGTTATGGTG 
TTCATCTATG GAGGTGGCTT CCTGCTTGGC GGTGGTCAGG GAGCAAACTT CTTGGACAAC 
TACCTGTATG ATGGAGAGGA GATTGCAGAT CGAGGTAATG TCATCGTGGT GACGTTCAAC 
TACCGTGTCG GATCCATGGG ATTCCTCAGC ACTGGAGATG CTGATGCACC TGGTAACTAT 
GGACTGTGGG ATCAGCATGC TGCAATCTCC TGGGTTCACA GGAACATCAA AGCTTTCGGT 
GGAAATCCAG ACAACATCAC CATCTTTGGG GAGTCAGCAG GGTCTACCAG TGTCAACTTC 
CAGATTATTT CACCTAAAAA CAAGGGCCTG ATCCGCAGAG CCATCTCTCA GAGTGGCGTT 
GCTCTCTGTC CCTGGGCGAT CAATAGAAAT CCCCGAAAAT TTGCTGAAGA GATCGCCAAG 
AAAGTAGGAT GCCCCACTGA TAGCGGGATG GTGGCCTGTC TGAGAAGGAC AGATCCGAAG 
GCTGTAACTC TCGCTGGGAA AGTGAGGCTT GCTACATCTG CTACTGAACC CATCGTGCAC 
AATCTGTATC TGTCCCCCGT AATCGATGGC GATTTCATCC CTGATGAGCC TGACACTCTT 
TTCGGAAATG CTGCTGACAT CGACTACATC GCTGGAGTCA ATGACATGGA TGGACACATC 
TTTGCCACCC TTGACATTCC CTCAATCAAC AACGCTCTGG CGACCACAAC TGAAGAGGAG 
GTCCAGGCTC TCGCTACTGC CCTCTCCAAA GACAGAGGCC AGGATGCTGG AATTGCAACT 
TTTCAGGAGT ACACAGTTAA TTGGGGAAGT AAACCTAATA AAGAGGATAT TAAGAAGACA 
GTTGTGGAAA TGGAGACAGA CTACATCTTC CTGGTGCCAA CTCAAACAGC CCTGTACCTC 
CATAGTGACA ATGCCAAATC TGCCCGAACC TACTCGTACC TGTTCAGTGA GAGTTCTCGT 
ATTCCTATCT TCCCTCTGTG GATGGGCGCT GATCACGCTG ACGAACTTCA GTATGTGTTT 
GGAAAGCCTT TCACCACCCC TTTGGGCTAT TTTCCTCGTC ATCGGGACGT CTCCAAATAC 
ATGATTGCCT ACTGGACCAA TTTTGCTCAA ACTGGGAATC CTAACACAGG TGAGTCCAAG 
GTTCCCGTAA CCTGGCCTGA GTTCTCCAAC CCTGGCCATC AGTATCTGGA CATCAACAAC 
AAGATAAACA CGAACAGTGT GAAGAAGATG TTGAGAACTC GCTTGGTTTA CTACTGGACC 
ACCGTCTTTG CCAGTTACCC TACTGTCAGG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020344.2
CDS	30..1685
Translation	MMMMLGVFISAALFLGTAQGASLGAVLTEGGMVQGKSHSVGLLRYMDVFKGIPFAAPPGRLQKPVPHPGWEGVLKATEYSKRCIQLNLLQTDTMGSEDCLYLNIWVPQGKTVSYNLPVMVFIYGGGFLLGGGQGANFLDNYLYDGEEIADRGNVIVVTFNYRVGSMGFLSTGDADAPGNYGLWDQHAAISWVHRNIKAFGGNPDNITIFGESAGSTSVNFQIISPKNKGLIRRAISQSGVALCPWAINRNPRKFAEEIAKKVGCPTDSGMVACLRRTDPKAVTLAGKVRLATSATEPIVHNLYLSPVIDGDFIPDEPDTLFGNAADIDYIAGVNDMDGHIFATLDIPSINNALATTTEEEVQALATALSKDRGQDAGIATFQEYTVNWGSKPNKEDIKKTVVEMETDYIFLVPTQTALYLHSDNAKSARTYSYLFSESSRIPIFPLWMGADHADELQYVFGKPFTTPLGYFPRHRDVSKYMIAYWTNFAQTGNPNTGESKVPVTWPEFSNPGHQYLDINNKINTNSVKKMLRTRLVYYWTTVFASYPTVRN

Target ORF information:

RefSeq Version	NM_001025173.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio carboxyl ester lipase, tandem duplicate 2 (cel.2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025173.2

ORF Insert Sequence:

ATGATGATGA TGCTGGGAGT TTTCATCTCT GCCGCCCTGT TCCTAGGGAC AGCACAAGGA 
GCTTCTTTGG GAGCTGTGCT CACTGAAGGA GGGATGGTGC AGGGAAAGAG TCATAGTGTT 
GGTCTTCTCC GCTACATGGA TGTCTTTAAG GGTATCCCCT TTGCCGCTCC ACCTGGCAGA 
CTGCAGAAGC CGGTTCCTCA CCCTGGCTGG GAAGGTGTCC TGAAAGCCAC TGAATATAGT 
AAGAGGTGTA TTCAGCTCAA TCTCCTTCAA ACGGATACCA TGGGCAGTGA AGACTGTCTG 
TACCTGAACA TATGGGTCCC TCAAGGCAAA ACTGTTTCCT ACAACTTGCC TGTTATGGTG 
TTCATCTATG GAGGTGGCTT CCTGCTTGGC GGTGGTCAGG GAGCAAACTT CTTGGACAAC 
TACCTGTATG ATGGAGAGGA GATTGCAGAT CGAGGTAATG TCATCGTGGT GACGTTCAAC 
TACCGTGTCG GATCCATGGG ATTCCTCAGC ACTGGAGATG CTGATGCACC TGGTAACTAT 
GGACTGTGGG ATCAGCATGC TGCAATCTCC TGGGTTCACA GGAACATCAA AGCTTTCGGT 
GGAAATCCAG ACAACATCAC CATCTTTGGG GAGTCAGCAG GGTCTACCAG TGTCAACTTC 
CAGATTATTT CACCTAAAAA CAAGGGCCTG ATCCGCAGAG CCATCTCTCA GAGTGGCGTT 
GCTCTCTGTC CCTGGGCGAT CAATAGAAAT CCCCGAAAAT TTGCTGAAGA GATCGCCAAG 
AAAGTAGGAT GCCCCACTGA TAGCGGGATG GTGGCCTGTC TGAGAAGGAC AGATCCGAAG 
GCTGTAACTC TCGCTGGGAA AGTGAGGCTT GCTACATCTG CTACTGAACC CATCGTGCAC 
AATCTGTATC TGTCCCCCGT AATCGATGGC GATTTCATCC CTGATGAGCC TGACACTCTT 
TTCGGAAATG CTGCTGACAT CGACTACATC GCTGGAGTCA ATGACATGGA TGGACACATC 
TTTGCCACCC TTGACATTCC CTCAATCAAC AACGCTCTGG CGACCACAAC TGAAGAGGAG 
GTCCAGGCTC TCGCTACTGC CCTCTCCAAA GACAGAGGCC AGGATGCTGG AATTGCAACT 
TTTCAGGAGT ACACAGTTAA TTGGGGAAGT AAACCTAATA AAGAGGATAT TAAGAAGACA 
GTTGTGGAAA TGGAGACAGA CTACATCTTC CTGGTGCCAA CTCAAACAGC CCTGTACCTC 
CATAGTGACA ATGCCAAATC TGCCCGAACC TACTCGTACC TGTTCAGTGA GAGTTCTCGT 
ATTCCTATCT TCCCTCTGTG GATGGGCGCT GATCACGCTG ACGAACTTCA GTATGTGTTT 
GGAAAGCCTT TCACCACCCC TTTGGGCTAT TTTCCTCGTC ATCGGGACGT CTCCAAATAC 
ATGATTGCCT ACTGGACCAA TTTTGCTCAA ACTGGGAATC CTAACACAGG TGAGTCCAAG 
GTTCCCGTAA CCTGGCCTGA GTTCTCCAAC CCTGGCCATC AGTATCTGGA CATCAACAAC 
AAGATAAACA CGAACAGTGT GAAGAAGATG TTGAGAACTC GCTTGGTTTA CTACTGGACC 
ACCGTCTTTG CCAGTTACCC TACTGTCAGG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cel.2 ( XM_021469026.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324701.1 Danio rerio carboxyl ester lipase, tandem duplicate 2 (cel.2), mRNA.

Price & Availability↑

CloneID	ODa25663
Clone ID Related Accession (Same CDS sequence)	XM_021469026.1 , XM_009295234.2 , NM_001025173.2
Accession Version	XM_021469026.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	bile salt-activated lipase
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGCTGGGAGT TTTCATCTCT GCCGCCCTGT TCCTAGGGAC AGCACAAGGA 
GCTTCTTTGG GAGCTGTGCT CACTGAAGGA GGGATGGTGC AGGGAAAGAG TCATAGTGTT 
GGTCTTCTCC GCTACATGGA TGTCTTTAAG GGTATCCCCT TTGCCGCTCC ACCTGGCAGA 
CTGCAGAAGC CGGTTCCTCA CCCTGGCTGG GAAGGTGTCC TGAAAGCCAC TGAATATAGT 
AAGAGGTGTA TTCAGCTCAA TCTCCTTCAA ACGGATACCA TGGGCAGTGA AGACTGTCTG 
TACCTGAACA TATGGGTCCC TCAAGGCAAA ACTGTTTCCT ACAACTTGCC TGTTATGGTG 
TTCATCTATG GAGGTGGCTT CCTGCTTGGC GGTGGTCAGG GAGCAAACTT CTTGGACAAC 
TACCTGTATG ATGGAGAGGA GATTGCAGAT CGAGGTAATG TCATCGTGGT GACGTTCAAC 
TACCGTGTCG GATCCATGGG ATTCCTCAGC ACTGGAGATG CTGATGCACC TGGTAACTAT 
GGACTGTGGG ATCAGCATGC TGCAATCTCC TGGGTTCACA GGAACATCAA AGCTTTCGGT 
GGAAATCCAG ACAACATCAC CATCTTTGGG GAGTCAGCAG GGTCTACCAG TGTCAACTTC 
CAGATTATTT CACCTAAAAA CAAGGGCCTG ATCCGCAGAG CCATCTCTCA GAGTGGCGTT 
GCTCTCTGTC CCTGGGCGAT CAATAGAAAT CCCCGAAAAT TTGCTGAAGA GATCGCCAAG 
AAAGTAGGAT GCCCCACTGA TAGCGGGATG GTGGCCTGTC TGAGAAGGAC AGATCCGAAG 
GCTGTAACTC TCGCTGGGAA AGTGAGGCTT GCTACATCTG CTACTGAACC CATCGTGCAC 
AATCTGTATC TGTCCCCCGT AATCGATGGC GATTTCATCC CTGATGAGCC TGACACTCTT 
TTCGGAAATG CTGCTGACAT CGACTACATC GCTGGAGTCA ATGACATGGA TGGACACATC 
TTTGCCACCC TTGACATTCC CTCAATCAAC AACGCTCTGG CGACCACAAC TGAAGAGGAG 
GTCCAGGCTC TCGCTACTGC CCTCTCCAAA GACAGAGGCC AGGATGCTGG AATTGCAACT 
TTTCAGGAGT ACACAGTTAA TTGGGGAAGT AAACCTAATA AAGAGGATAT TAAGAAGACA 
GTTGTGGAAA TGGAGACAGA CTACATCTTC CTGGTGCCAA CTCAAACAGC CCTGTACCTC 
CATAGTGACA ATGCCAAATC TGCCCGAACC TACTCGTACC TGTTCAGTGA GAGTTCTCGT 
ATTCCTATCT TCCCTCTGTG GATGGGCGCT GATCACGCTG ACGAACTTCA GTATGTGTTT 
GGAAAGCCTT TCACCACCCC TTTGGGCTAT TTTCCTCGTC ATCGGGACGT CTCCAAATAC 
ATGATTGCCT ACTGGACCAA TTTTGCTCAA ACTGGGAATC CTAACACAGG TGAGTCCAAG 
GTTCCCGTAA CCTGGCCTGA GTTCTCCAAC CCTGGCCATC AGTATCTGGA CATCAACAAC 
AAGATAAACA CGAACAGTGT GAAGAAGATG TTGAGAACTC GCTTGGTTTA CTACTGGACC 
ACCGTCTTTG CCAGTTACCC TACTGTCAGG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324701.1
CDS	37..1692
Translation	MMMMLGVFISAALFLGTAQGASLGAVLTEGGMVQGKSHSVGLLRYMDVFKGIPFAAPPGRLQKPVPHPGWEGVLKATEYSKRCIQLNLLQTDTMGSEDCLYLNIWVPQGKTVSYNLPVMVFIYGGGFLLGGGQGANFLDNYLYDGEEIADRGNVIVVTFNYRVGSMGFLSTGDADAPGNYGLWDQHAAISWVHRNIKAFGGNPDNITIFGESAGSTSVNFQIISPKNKGLIRRAISQSGVALCPWAINRNPRKFAEEIAKKVGCPTDSGMVACLRRTDPKAVTLAGKVRLATSATEPIVHNLYLSPVIDGDFIPDEPDTLFGNAADIDYIAGVNDMDGHIFATLDIPSINNALATTTEEEVQALATALSKDRGQDAGIATFQEYTVNWGSKPNKEDIKKTVVEMETDYIFLVPTQTALYLHSDNAKSARTYSYLFSESSRIPIFPLWMGADHADELQYVFGKPFTTPLGYFPRHRDVSKYMIAYWTNFAQTGNPNTGESKVPVTWPEFSNPGHQYLDINNKINTNSVKKMLRTRLVYYWTTVFASYPTVRN

Target ORF information:

RefSeq Version	XM_021469026.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio carboxyl ester lipase, tandem duplicate 2 (cel.2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469026.1

ORF Insert Sequence:

ATGATGATGA TGCTGGGAGT TTTCATCTCT GCCGCCCTGT TCCTAGGGAC AGCACAAGGA 
GCTTCTTTGG GAGCTGTGCT CACTGAAGGA GGGATGGTGC AGGGAAAGAG TCATAGTGTT 
GGTCTTCTCC GCTACATGGA TGTCTTTAAG GGTATCCCCT TTGCCGCTCC ACCTGGCAGA 
CTGCAGAAGC CGGTTCCTCA CCCTGGCTGG GAAGGTGTCC TGAAAGCCAC TGAATATAGT 
AAGAGGTGTA TTCAGCTCAA TCTCCTTCAA ACGGATACCA TGGGCAGTGA AGACTGTCTG 
TACCTGAACA TATGGGTCCC TCAAGGCAAA ACTGTTTCCT ACAACTTGCC TGTTATGGTG 
TTCATCTATG GAGGTGGCTT CCTGCTTGGC GGTGGTCAGG GAGCAAACTT CTTGGACAAC 
TACCTGTATG ATGGAGAGGA GATTGCAGAT CGAGGTAATG TCATCGTGGT GACGTTCAAC 
TACCGTGTCG GATCCATGGG ATTCCTCAGC ACTGGAGATG CTGATGCACC TGGTAACTAT 
GGACTGTGGG ATCAGCATGC TGCAATCTCC TGGGTTCACA GGAACATCAA AGCTTTCGGT 
GGAAATCCAG ACAACATCAC CATCTTTGGG GAGTCAGCAG GGTCTACCAG TGTCAACTTC 
CAGATTATTT CACCTAAAAA CAAGGGCCTG ATCCGCAGAG CCATCTCTCA GAGTGGCGTT 
GCTCTCTGTC CCTGGGCGAT CAATAGAAAT CCCCGAAAAT TTGCTGAAGA GATCGCCAAG 
AAAGTAGGAT GCCCCACTGA TAGCGGGATG GTGGCCTGTC TGAGAAGGAC AGATCCGAAG 
GCTGTAACTC TCGCTGGGAA AGTGAGGCTT GCTACATCTG CTACTGAACC CATCGTGCAC 
AATCTGTATC TGTCCCCCGT AATCGATGGC GATTTCATCC CTGATGAGCC TGACACTCTT 
TTCGGAAATG CTGCTGACAT CGACTACATC GCTGGAGTCA ATGACATGGA TGGACACATC 
TTTGCCACCC TTGACATTCC CTCAATCAAC AACGCTCTGG CGACCACAAC TGAAGAGGAG 
GTCCAGGCTC TCGCTACTGC CCTCTCCAAA GACAGAGGCC AGGATGCTGG AATTGCAACT 
TTTCAGGAGT ACACAGTTAA TTGGGGAAGT AAACCTAATA AAGAGGATAT TAAGAAGACA 
GTTGTGGAAA TGGAGACAGA CTACATCTTC CTGGTGCCAA CTCAAACAGC CCTGTACCTC 
CATAGTGACA ATGCCAAATC TGCCCGAACC TACTCGTACC TGTTCAGTGA GAGTTCTCGT 
ATTCCTATCT TCCCTCTGTG GATGGGCGCT GATCACGCTG ACGAACTTCA GTATGTGTTT 
GGAAAGCCTT TCACCACCCC TTTGGGCTAT TTTCCTCGTC ATCGGGACGT CTCCAAATAC 
ATGATTGCCT ACTGGACCAA TTTTGCTCAA ACTGGGAATC CTAACACAGG TGAGTCCAAG 
GTTCCCGTAA CCTGGCCTGA GTTCTCCAAC CCTGGCCATC AGTATCTGGA CATCAACAAC 
AAGATAAACA CGAACAGTGT GAAGAAGATG TTGAGAACTC GCTTGGTTTA CTACTGGACC 
ACCGTCTTTG CCAGTTACCC TACTGTCAGG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cel.2 ( XM_009295234.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009293509.1 Danio rerio carboxyl ester lipase, tandem duplicate 2 (cel.2), mRNA.

Price & Availability↑

CloneID	ODa25663
Clone ID Related Accession (Same CDS sequence)	XM_021469026.1 , XM_009295234.2 , NM_001025173.2
Accession Version	XM_009295234.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	bile salt-activated lipase
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009295234.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGATGA TGCTGGGAGT TTTCATCTCT GCCGCCCTGT TCCTAGGGAC AGCACAAGGA 
GCTTCTTTGG GAGCTGTGCT CACTGAAGGA GGGATGGTGC AGGGAAAGAG TCATAGTGTT 
GGTCTTCTCC GCTACATGGA TGTCTTTAAG GGTATCCCCT TTGCCGCTCC ACCTGGCAGA 
CTGCAGAAGC CGGTTCCTCA CCCTGGCTGG GAAGGTGTCC TGAAAGCCAC TGAATATAGT 
AAGAGGTGTA TTCAGCTCAA TCTCCTTCAA ACGGATACCA TGGGCAGTGA AGACTGTCTG 
TACCTGAACA TATGGGTCCC TCAAGGCAAA ACTGTTTCCT ACAACTTGCC TGTTATGGTG 
TTCATCTATG GAGGTGGCTT CCTGCTTGGC GGTGGTCAGG GAGCAAACTT CTTGGACAAC 
TACCTGTATG ATGGAGAGGA GATTGCAGAT CGAGGTAATG TCATCGTGGT GACGTTCAAC 
TACCGTGTCG GATCCATGGG ATTCCTCAGC ACTGGAGATG CTGATGCACC TGGTAACTAT 
GGACTGTGGG ATCAGCATGC TGCAATCTCC TGGGTTCACA GGAACATCAA AGCTTTCGGT 
GGAAATCCAG ACAACATCAC CATCTTTGGG GAGTCAGCAG GGTCTACCAG TGTCAACTTC 
CAGATTATTT CACCTAAAAA CAAGGGCCTG ATCCGCAGAG CCATCTCTCA GAGTGGCGTT 
GCTCTCTGTC CCTGGGCGAT CAATAGAAAT CCCCGAAAAT TTGCTGAAGA GATCGCCAAG 
AAAGTAGGAT GCCCCACTGA TAGCGGGATG GTGGCCTGTC TGAGAAGGAC AGATCCGAAG 
GCTGTAACTC TCGCTGGGAA AGTGAGGCTT GCTACATCTG CTACTGAACC CATCGTGCAC 
AATCTGTATC TGTCCCCCGT AATCGATGGC GATTTCATCC CTGATGAGCC TGACACTCTT 
TTCGGAAATG CTGCTGACAT CGACTACATC GCTGGAGTCA ATGACATGGA TGGACACATC 
TTTGCCACCC TTGACATTCC CTCAATCAAC AACGCTCTGG CGACCACAAC TGAAGAGGAG 
GTCCAGGCTC TCGCTACTGC CCTCTCCAAA GACAGAGGCC AGGATGCTGG AATTGCAACT 
TTTCAGGAGT ACACAGTTAA TTGGGGAAGT AAACCTAATA AAGAGGATAT TAAGAAGACA 
GTTGTGGAAA TGGAGACAGA CTACATCTTC CTGGTGCCAA CTCAAACAGC CCTGTACCTC 
CATAGTGACA ATGCCAAATC TGCCCGAACC TACTCGTACC TGTTCAGTGA GAGTTCTCGT 
ATTCCTATCT TCCCTCTGTG GATGGGCGCT GATCACGCTG ACGAACTTCA GTATGTGTTT 
GGAAAGCCTT TCACCACCCC TTTGGGCTAT TTTCCTCGTC ATCGGGACGT CTCCAAATAC 
ATGATTGCCT ACTGGACCAA TTTTGCTCAA ACTGGGAATC CTAACACAGG TGAGTCCAAG 
GTTCCCGTAA CCTGGCCTGA GTTCTCCAAC CCTGGCCATC AGTATCTGGA CATCAACAAC 
AAGATAAACA CGAACAGTGT GAAGAAGATG TTGAGAACTC GCTTGGTTTA CTACTGGACC 
ACCGTCTTTG CCAGTTACCC TACTGTCAGG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009293509.1
CDS	21..1676
Translation	MMMMLGVFISAALFLGTAQGASLGAVLTEGGMVQGKSHSVGLLRYMDVFKGIPFAAPPGRLQKPVPHPGWEGVLKATEYSKRCIQLNLLQTDTMGSEDCLYLNIWVPQGKTVSYNLPVMVFIYGGGFLLGGGQGANFLDNYLYDGEEIADRGNVIVVTFNYRVGSMGFLSTGDADAPGNYGLWDQHAAISWVHRNIKAFGGNPDNITIFGESAGSTSVNFQIISPKNKGLIRRAISQSGVALCPWAINRNPRKFAEEIAKKVGCPTDSGMVACLRRTDPKAVTLAGKVRLATSATEPIVHNLYLSPVIDGDFIPDEPDTLFGNAADIDYIAGVNDMDGHIFATLDIPSINNALATTTEEEVQALATALSKDRGQDAGIATFQEYTVNWGSKPNKEDIKKTVVEMETDYIFLVPTQTALYLHSDNAKSARTYSYLFSESSRIPIFPLWMGADHADELQYVFGKPFTTPLGYFPRHRDVSKYMIAYWTNFAQTGNPNTGESKVPVTWPEFSNPGHQYLDINNKINTNSVKKMLRTRLVYYWTTVFASYPTVRN

Target ORF information:

RefSeq Version	XM_009295234.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio carboxyl ester lipase, tandem duplicate 2 (cel.2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009295234.2

ORF Insert Sequence:

ATGATGATGA TGCTGGGAGT TTTCATCTCT GCCGCCCTGT TCCTAGGGAC AGCACAAGGA 
GCTTCTTTGG GAGCTGTGCT CACTGAAGGA GGGATGGTGC AGGGAAAGAG TCATAGTGTT 
GGTCTTCTCC GCTACATGGA TGTCTTTAAG GGTATCCCCT TTGCCGCTCC ACCTGGCAGA 
CTGCAGAAGC CGGTTCCTCA CCCTGGCTGG GAAGGTGTCC TGAAAGCCAC TGAATATAGT 
AAGAGGTGTA TTCAGCTCAA TCTCCTTCAA ACGGATACCA TGGGCAGTGA AGACTGTCTG 
TACCTGAACA TATGGGTCCC TCAAGGCAAA ACTGTTTCCT ACAACTTGCC TGTTATGGTG 
TTCATCTATG GAGGTGGCTT CCTGCTTGGC GGTGGTCAGG GAGCAAACTT CTTGGACAAC 
TACCTGTATG ATGGAGAGGA GATTGCAGAT CGAGGTAATG TCATCGTGGT GACGTTCAAC 
TACCGTGTCG GATCCATGGG ATTCCTCAGC ACTGGAGATG CTGATGCACC TGGTAACTAT 
GGACTGTGGG ATCAGCATGC TGCAATCTCC TGGGTTCACA GGAACATCAA AGCTTTCGGT 
GGAAATCCAG ACAACATCAC CATCTTTGGG GAGTCAGCAG GGTCTACCAG TGTCAACTTC 
CAGATTATTT CACCTAAAAA CAAGGGCCTG ATCCGCAGAG CCATCTCTCA GAGTGGCGTT 
GCTCTCTGTC CCTGGGCGAT CAATAGAAAT CCCCGAAAAT TTGCTGAAGA GATCGCCAAG 
AAAGTAGGAT GCCCCACTGA TAGCGGGATG GTGGCCTGTC TGAGAAGGAC AGATCCGAAG 
GCTGTAACTC TCGCTGGGAA AGTGAGGCTT GCTACATCTG CTACTGAACC CATCGTGCAC 
AATCTGTATC TGTCCCCCGT AATCGATGGC GATTTCATCC CTGATGAGCC TGACACTCTT 
TTCGGAAATG CTGCTGACAT CGACTACATC GCTGGAGTCA ATGACATGGA TGGACACATC 
TTTGCCACCC TTGACATTCC CTCAATCAAC AACGCTCTGG CGACCACAAC TGAAGAGGAG 
GTCCAGGCTC TCGCTACTGC CCTCTCCAAA GACAGAGGCC AGGATGCTGG AATTGCAACT 
TTTCAGGAGT ACACAGTTAA TTGGGGAAGT AAACCTAATA AAGAGGATAT TAAGAAGACA 
GTTGTGGAAA TGGAGACAGA CTACATCTTC CTGGTGCCAA CTCAAACAGC CCTGTACCTC 
CATAGTGACA ATGCCAAATC TGCCCGAACC TACTCGTACC TGTTCAGTGA GAGTTCTCGT 
ATTCCTATCT TCCCTCTGTG GATGGGCGCT GATCACGCTG ACGAACTTCA GTATGTGTTT 
GGAAAGCCTT TCACCACCCC TTTGGGCTAT TTTCCTCGTC ATCGGGACGT CTCCAAATAC 
ATGATTGCCT ACTGGACCAA TTTTGCTCAA ACTGGGAATC CTAACACAGG TGAGTCCAAG 
GTTCCCGTAA CCTGGCCTGA GTTCTCCAAC CCTGGCCATC AGTATCTGGA CATCAACAAC 
AAGATAAACA CGAACAGTGT GAAGAAGATG TTGAGAACTC GCTTGGTTTA CTACTGGACC 
ACCGTCTTTG CCAGTTACCC TACTGTCAGG AACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The draft genome of the grass carp (Ctenopharyngodon idellus) provides insights into its evolution and vegetarian adaptation.
Nature genetics47(6)625-31(2015 Jun)
Wang Y,Lu Y,Zhang Y,Ning Z,Li Y,Zhao Q,Lu H,Huang R,Xia X,Feng Q,Liang X,Liu K,Zhang L,Lu T,Huang T,Fan D,Weng Q,Zhu C,Lu Y,Li W,Wen Z,Zhou C,Tian Q,Kang X,Shi M,Zhang W,Jang S,Du F,He S,Liao L,Li Y,Gui B,He H,Ning Z,Yang C,He L,Luo L,Yang R,Luo Q,Liu X,Li S,Huang W,Xiao L,Lin H,Han B,Zhu Z

Systems pharmacology of adiposity reveals inhibition of EP300 as a common therapeutic mechanism of caloric restriction and resveratrol for obesity.
Frontiers in pharmacology6199(2015)
Nishimura Y,Sasagawa S,Ariyoshi M,Ichikawa S,Shimada Y,Kawaguchi K,Kawase R,Yamamoto R,Uehara T,Yanai T,Takata R,Tanaka T

Zebrafish acetylcholinesterase is encoded by a single gene localized on linkage group 7. Gene structure and polymorphism; molecular forms and expression pattern during development.
The Journal of biological chemistry276(1)464-74(2001 Jan)
Bertrand C,Chatonnet A,Takke C,Yan YL,Postlethwait J,Toutant JP,Cousin X

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cel.2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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