znf827 gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_005159980.4	XP_005160037.1	zinc finger protein 827 isoform X4
XM_009292558.2	XP_009290833.1	zinc finger protein 827 isoform X3
XM_005159979.3	XP_005160036.1	zinc finger protein 827 isoform X4
XM_001919166.7	XP_001919201.4	zinc finger protein 827 isoform X2
XM_001919166.6	XP_001919201.4	zinc finger protein 827 isoform X2
XM_009292558.3	XP_009290833.1	zinc finger protein 827 isoform X3
XM_005159979.4	XP_005160036.1	zinc finger protein 827 isoform X4
XM_005159977.3	XP_005160034.1	zinc finger protein 827 isoform X1
XM_005159980.3	XP_005160037.1	zinc finger protein 827 isoform X4
XM_005159977.4	XP_005160034.1	zinc finger protein 827 isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa05910	XM_005159980.4 Latest version!	Danio rerio zinc finger protein 827 (znf827), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa05910	XM_005159979.4 Latest version!	Danio rerio zinc finger protein 827 (znf827), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa05908	XM_005159977.4 Latest version!	Danio rerio zinc finger protein 827 (znf827), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa05909	XM_001919166.7 Latest version!	Danio rerio zinc finger protein 827 (znf827), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44462	XM_009292558.3 Latest version!	Danio rerio zinc finger protein 827 (znf827), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa05909	XM_001919166.6	Danio rerio zinc finger protein 827 (znf827), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa44462	XM_009292558.2	Danio rerio zinc finger protein 827 (znf827), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa05910	XM_005159979.3	Danio rerio zinc finger protein 827 (znf827), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa05910	XM_005159980.3	Danio rerio zinc finger protein 827 (znf827), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa05908	XM_005159977.3	Danio rerio zinc finger protein 827 (znf827), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

znf827 ( XM_005159980.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160037.1 Danio rerio zinc finger protein 827 (znf827), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa05910
Clone ID Related Accession (Same CDS sequence)	XM_005159980.4 , XM_005159979.3 , XM_005159979.4 , XM_005159980.3
Accession Version	XM_005159980.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	zinc finger protein 827 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159980.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GGAAACAGGA GCAACCCAAG CGCCTGCCTT CACATGTGGA CACAGTAGAG 
GATGGAGGGC CAGAGAGTGG CTCAGTAGAG GAGGACAGCT GGTTTGGGAA TACCTCCGAC 
AGCCCCTCGG AATCGTCCTA CGGTGATGTG CAAGATGAGC TTCGGGTTTC TCCAGACAAC 
GTTCCCCGAC CGGACCACGA CACCTCAGCT GAGAGCTCCC TGGGCTGCCC GACGCCAGTT 
CAGCGGGCCT CTCTGGAGCT TCTGGGCCTG GACAGTGGGG TCCTTTCGAC CCAGGACACG 
CTTTCCTCTG TGGTGTCCTC TCTTTATGGA GACTCTAGCC AGGCTCTGGG TAAGCCACTC 
AGCAGTAATC TGCGCCGCCT CCTGGAGGCT GGCTCACTGA AGCTTGAGGG AGGGGAGCTG 
GCTGGACGTG GAACGGGTAG AGGACAAGAG TCACCCCCGG TCAGCCTGGT ACTATCTCCG 
TCTTCACATC ACGCACAGCA GCTGAGTGCC CTGGCGAGGA GGTTGGCAAA TAGCAGCAAC 
AACAGCGGAA ATTCAAACTC TGCGTCACCT GCATCTGTAG CAACTGTCAA GCAGGAGCCC 
TGTGACCCAT ACGGCCCCAG CAACGGCCCT AACTCAGGCA GCAGTGGCTT TGTATGGGTA 
GGCGTCGCAG ATAAATGGCC ATCGACAAGT TGCTCAGCAC CCAGTGGCAG TAGCCTCTCA 
CCAGACTCAG CAATTCAGAA GTTAAAGGCA GCCGCCAACG CCGTGCTCCA AGACAAGAAC 
GCGGTGGCCT CCTCCACCTC GGCATCTTCA TCCTCCTCGT CCTCCTCCTC ATCCTCTTCA 
GTCACCTCCT CCTCAATGGC AGCCCTGGGC ACTCGCTCTG ATGACGCAGT GCGCTTTGAA 
CCATTCGCTT CACCCTTTAG CCCGCAGTCA GCCAGCTCTA CGCTTGCTGC CCTCTCAAAG 
AAAGTGTCTG AAAGAAGTCA GCAGCAGGTG GGCTCATCTG AGCATCAACC CTCAGCTGGC 
TCCTTTCTCT CTCTGGTTTC CATGACCTCC TCAGCAGCTC TGCTAAAGGA GGTGGCCGCA 
CGAGCAGCAG GGAATCTTCT TGCTGATAAG AAAGAGCCGG CGTTAGTACT GGGAGCCTCC 
GAAGATGTCA AGCCCCTGTT AGACAGGAAC CAGAAGGTGC CCACCCCAAC CCAGGCAATG 
GATCTTCTGC TGCCAACAGT CCCCAAGGGA AGGGCCAAGC CCAGCAGTCA AGCAGGTTCT 
CCTGAGGATG GAGGAAAGCC CTTTCAGTGT CCGGTATGCG GTCTGGTCAT CAAGAGAAAG 
AGCTACTGGA AGAGACATAT GGTGATCCAT ACAGGCTTAA AAAGCCACCA GTGTCCCCTC 
TGTCCCTTTC GCTGTGCTCG TAAAGACAAT CTCAAGTCCC ACATGAAGGT CCATCAGCAC 
CAAGACCGAG GGGAGACGTT CCAGTGTGAG CTCTGCCCCT TCACATCCTC CCGCCACTTT 
AGCCTCAAGC TGCACATGCG TTGCCATCAG CACTTCCCTC GTCCCGACCT CAAAGTCAAA 
GAAGAACCCG GCACAGACAC CGAGGAGGGC GAGGGCTTGC TCATGGGGGA CGGTGGAGCG 
GCTGGTGATC AGGCCATGAA CACAGATGCT TCTACGTCTC CCACTGTGAG CCAACCCGAC 
GCTCGTTTGC TGGCCTCTCC TGCAGAACCC TCAAACCACG TCCAGATCAA GGAGGAACCA 
CAGGAGAGGG ATGTGTCTGT GATGTCTCCC TTTGCCCTGT GTAGGGAGAG GCCGAGCAGC 
AGCAGCAGTT CTCTGGATCT GTCCGGGCTT AAATCTAGTC CACCGGGGCC CGGGCCTACA 
GCTGCTTCAC TCTTCAGCCC AGATATCACC ACCAAGACGG CTACAGACCT CCTCATAAAG 
CTCTCGGCAG CGAACCAGAA GGAGGCCCTA AAGGGTCCAT ACTGTGTCAA GGAAGAGCCC 
CAGGCGGAGG AGCCCCTGAA CTCTCAGAGG GGACCCAGCT ACAGCTTCTG CCAAGAAGGA 
CCTCCAGGAG CCACCAAACC AAGAGAGGAG GGGAGCCCGA TGTTGGGCAA GAGCAGCCTT 
CTCAGCCAGG ACATCAACGT CAAAGTGGCC TCTGAATTGC TTATGAAGCT CTCAGAGAAC 
AACAAAGACG CTTGCGTCCA GAATGTTATT GTGAAGGCGG AGCCTATGGA AGTGGATCCA 
CCCCTTGATC TGGCCTCACC CTCCTCCCAT CTACTGGGCT TTAGCACTTC AGGACCATGT 
GAAAAGAAGG AACCAATGGT CAACCTCCCA GATCATTTGC CCCGCCCCCA GAATGACCTC 
TTCTCGCAGG ACATATCGGT CAAAATGGCC TCCGAGCTGC TTTTCAAACT CTCTGAAAAA 
GTAAGTAAAG CTAATGAAAA CAAAGACAAT GCATCATTCG GTATACACAG CCCTTTCCTT 
GATGACCGCT TTAGACAATC ACCTTTCAGC ACTCGCTCCA AGAGCTCTTC CCCAGCTGAA 
GCCGGATCTT CAAGCAGGGT TCCCCAGCAA GATCCAGAGA AGGTGAGTAG TGAGCCAGGA 
AATGGACTTT CCCAATGGAG ACTCAACGAA CAGCTCTTTC CCTGCCCTGT GTGTGGCAAA 
GTGTTTGGTC GCCAACAGAC TCTCTCCAGG CATCTCTCCT TACACACAGA AGAGAGGAAA 
TACAAGTGTC ATTTGTGTCC ATATGCAGCT AAGTGCCGAG CCAACCTCAA CCAGCACCTC 
ACCATCCACT CAGTCAAACT GGTGAACACA GATGTCGAGC AGATTGTTAC CGCTGTTACC 
AACGATGGCC CAGAGCTCAA AAACTGCCCC TACTACTACA GCTGCCATGT CTGCGGCTTC 
CAGACAGAGT TCAATGCTCA GTTTGTCAGT CATATGTCTC TTCATGTGGA CAAAGAGCAG 
TGGATGTTTT CTCTCTGCTG TAGCACCTGT GAGTATGTGG GTGTGGACGA GGCCGAGATG 
AAAGCTCACA TCAGTGCAGG GCACGCAGGT CTCAATTCTA GAAGCCCTCT CAGTGAGACC 
AAGAGCACTT CCTCCTCTCT GTCAGCCCTC AGCGACTCCC TCAACAGCTC TGAAGGTGGA 
GATGTTGCCC AAGGTAACGA AGAACTAAAA AGCCTTTTGG CTCCGCCTTC ATCTGTGGGA 
AGCCAATCAA GCTTAGGCCC AGGAGCAGAG GAAAAAGCAG AGAAAGGCTT TGAATGCGTC 
TTCTGCAACT TTGTGTGTAA AACCCGACCC ATGTACGAGC GGCACCTACA GATCCACCTG 
ATCACGCGCA TGTTTGAGTG TGACGTCTGC CACAAGTTCA TGAAGACGCC CGAGCAGCTC 
CTGGAGCACA AGAAGTGCCA CACCGTCCCC GCTGGAGGGC TCAAGGTTTG TGAAGCGCTT 
TGGGGCCCTG AGCAGATGAA AGGTGCTATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160037.1
CDS	415..3867
Translation	MPRRKQEQPKRLPSHVDTVEDGGPESGSVEEDSWFGNTSDSPSESSYGDVQDELRVSPDNVPRPDHDTSAESSLGCPTPVQRASLELLGLDSGVLSTQDTLSSVVSSLYGDSSQALGKPLSSNLRRLLEAGSLKLEGGELAGRGTGRGQESPPVSLVLSPSSHHAQQLSALARRLANSSNNSGNSNSASPASVATVKQEPCDPYGPSNGPNSGSSGFVWVGVADKWPSTSCSAPSGSSLSPDSAIQKLKAAANAVLQDKNAVASSTSASSSSSSSSSSSSVTSSSMAALGTRSDDAVRFEPFASPFSPQSASSTLAALSKKVSERSQQQVGSSEHQPSAGSFLSLVSMTSSAALLKEVAARAAGNLLADKKEPALVLGASEDVKPLLDRNQKVPTPTQAMDLLLPTVPKGRAKPSSQAGSPEDGGKPFQCPVCGLVIKRKSYWKRHMVIHTGLKSHQCPLCPFRCARKDNLKSHMKVHQHQDRGETFQCELCPFTSSRHFSLKLHMRCHQHFPRPDLKVKEEPGTDTEEGEGLLMGDGGAAGDQAMNTDASTSPTVSQPDARLLASPAEPSNHVQIKEEPQERDVSVMSPFALCRERPSSSSSSLDLSGLKSSPPGPGPTAASLFSPDITTKTATDLLIKLSAANQKEALKGPYCVKEEPQAEEPLNSQRGPSYSFCQEGPPGATKPREEGSPMLGKSSLLSQDINVKVASELLMKLSENNKDACVQNVIVKAEPMEVDPPLDLASPSSHLLGFSTSGPCEKKEPMVNLPDHLPRPQNDLFSQDISVKMASELLFKLSEKVSKANENKDNASFGIHSPFLDDRFRQSPFSTRSKSSSPAEAGSSSRVPQQDPEKVSSEPGNGLSQWRLNEQLFPCPVCGKVFGRQQTLSRHLSLHTEERKYKCHLCPYAAKCRANLNQHLTIHSVKLVNTDVEQIVTAVTNDGPELKNCPYYYSCHVCGFQTEFNAQFVSHMSLHVDKEQWMFSLCCSTCEYVGVDEAEMKAHISAGHAGLNSRSPLSETKSTSSSLSALSDSLNSSEGGDVAQGNEELKSLLAPPSSVGSQSSLGPGAEEKAEKGFECVFCNFVCKTRPMYERHLQIHLITRMFECDVCHKFMKTPEQLLEHKKCHTVPAGGLKVCEALWGPEQMKGAI

Target ORF information:

RefSeq Version	XM_005159980.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zinc finger protein 827 (znf827), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159980.4

ORF Insert Sequence:

ATGCCAAGGA GGAAACAGGA GCAACCCAAG CGCCTGCCTT CACATGTGGA CACAGTAGAG 
GATGGAGGGC CAGAGAGTGG CTCAGTAGAG GAGGACAGCT GGTTTGGGAA TACCTCCGAC 
AGCCCCTCGG AATCGTCCTA CGGTGATGTG CAAGATGAGC TTCGGGTTTC TCCAGACAAC 
GTTCCCCGAC CGGACCACGA CACCTCAGCT GAGAGCTCCC TGGGCTGCCC GACGCCAGTT 
CAGCGGGCCT CTCTGGAGCT TCTGGGCCTG GACAGTGGGG TCCTTTCGAC CCAGGACACG 
CTTTCCTCTG TGGTGTCCTC TCTTTATGGA GACTCTAGCC AGGCTCTGGG TAAGCCACTC 
AGCAGTAATC TGCGCCGCCT CCTGGAGGCT GGCTCACTGA AGCTTGAGGG AGGGGAGCTG 
GCTGGACGTG GAACGGGTAG AGGACAAGAG TCACCCCCGG TCAGCCTGGT ACTATCTCCG 
TCTTCACATC ACGCACAGCA GCTGAGTGCC CTGGCGAGGA GGTTGGCAAA TAGCAGCAAC 
AACAGCGGAA ATTCAAACTC TGCGTCACCT GCATCTGTAG CAACTGTCAA GCAGGAGCCC 
TGTGACCCAT ACGGCCCCAG CAACGGCCCT AACTCAGGCA GCAGTGGCTT TGTATGGGTA 
GGCGTCGCAG ATAAATGGCC ATCGACAAGT TGCTCAGCAC CCAGTGGCAG TAGCCTCTCA 
CCAGACTCAG CAATTCAGAA GTTAAAGGCA GCCGCCAACG CCGTGCTCCA AGACAAGAAC 
GCGGTGGCCT CCTCCACCTC GGCATCTTCA TCCTCCTCGT CCTCCTCCTC ATCCTCTTCA 
GTCACCTCCT CCTCAATGGC AGCCCTGGGC ACTCGCTCTG ATGACGCAGT GCGCTTTGAA 
CCATTCGCTT CACCCTTTAG CCCGCAGTCA GCCAGCTCTA CGCTTGCTGC CCTCTCAAAG 
AAAGTGTCTG AAAGAAGTCA GCAGCAGGTG GGCTCATCTG AGCATCAACC CTCAGCTGGC 
TCCTTTCTCT CTCTGGTTTC CATGACCTCC TCAGCAGCTC TGCTAAAGGA GGTGGCCGCA 
CGAGCAGCAG GGAATCTTCT TGCTGATAAG AAAGAGCCGG CGTTAGTACT GGGAGCCTCC 
GAAGATGTCA AGCCCCTGTT AGACAGGAAC CAGAAGGTGC CCACCCCAAC CCAGGCAATG 
GATCTTCTGC TGCCAACAGT CCCCAAGGGA AGGGCCAAGC CCAGCAGTCA AGCAGGTTCT 
CCTGAGGATG GAGGAAAGCC CTTTCAGTGT CCGGTATGCG GTCTGGTCAT CAAGAGAAAG 
AGCTACTGGA AGAGACATAT GGTGATCCAT ACAGGCTTAA AAAGCCACCA GTGTCCCCTC 
TGTCCCTTTC GCTGTGCTCG TAAAGACAAT CTCAAGTCCC ACATGAAGGT CCATCAGCAC 
CAAGACCGAG GGGAGACGTT CCAGTGTGAG CTCTGCCCCT TCACATCCTC CCGCCACTTT 
AGCCTCAAGC TGCACATGCG TTGCCATCAG CACTTCCCTC GTCCCGACCT CAAAGTCAAA 
GAAGAACCCG GCACAGACAC CGAGGAGGGC GAGGGCTTGC TCATGGGGGA CGGTGGAGCG 
GCTGGTGATC AGGCCATGAA CACAGATGCT TCTACGTCTC CCACTGTGAG CCAACCCGAC 
GCTCGTTTGC TGGCCTCTCC TGCAGAACCC TCAAACCACG TCCAGATCAA GGAGGAACCA 
CAGGAGAGGG ATGTGTCTGT GATGTCTCCC TTTGCCCTGT GTAGGGAGAG GCCGAGCAGC 
AGCAGCAGTT CTCTGGATCT GTCCGGGCTT AAATCTAGTC CACCGGGGCC CGGGCCTACA 
GCTGCTTCAC TCTTCAGCCC AGATATCACC ACCAAGACGG CTACAGACCT CCTCATAAAG 
CTCTCGGCAG CGAACCAGAA GGAGGCCCTA AAGGGTCCAT ACTGTGTCAA GGAAGAGCCC 
CAGGCGGAGG AGCCCCTGAA CTCTCAGAGG GGACCCAGCT ACAGCTTCTG CCAAGAAGGA 
CCTCCAGGAG CCACCAAACC AAGAGAGGAG GGGAGCCCGA TGTTGGGCAA GAGCAGCCTT 
CTCAGCCAGG ACATCAACGT CAAAGTGGCC TCTGAATTGC TTATGAAGCT CTCAGAGAAC 
AACAAAGACG CTTGCGTCCA GAATGTTATT GTGAAGGCGG AGCCTATGGA AGTGGATCCA 
CCCCTTGATC TGGCCTCACC CTCCTCCCAT CTACTGGGCT TTAGCACTTC AGGACCATGT 
GAAAAGAAGG AACCAATGGT CAACCTCCCA GATCATTTGC CCCGCCCCCA GAATGACCTC 
TTCTCGCAGG ACATATCGGT CAAAATGGCC TCCGAGCTGC TTTTCAAACT CTCTGAAAAA 
GTAAGTAAAG CTAATGAAAA CAAAGACAAT GCATCATTCG GTATACACAG CCCTTTCCTT 
GATGACCGCT TTAGACAATC ACCTTTCAGC ACTCGCTCCA AGAGCTCTTC CCCAGCTGAA 
GCCGGATCTT CAAGCAGGGT TCCCCAGCAA GATCCAGAGA AGGTGAGTAG TGAGCCAGGA 
AATGGACTTT CCCAATGGAG ACTCAACGAA CAGCTCTTTC CCTGCCCTGT GTGTGGCAAA 
GTGTTTGGTC GCCAACAGAC TCTCTCCAGG CATCTCTCCT TACACACAGA AGAGAGGAAA 
TACAAGTGTC ATTTGTGTCC ATATGCAGCT AAGTGCCGAG CCAACCTCAA CCAGCACCTC 
ACCATCCACT CAGTCAAACT GGTGAACACA GATGTCGAGC AGATTGTTAC CGCTGTTACC 
AACGATGGCC CAGAGCTCAA AAACTGCCCC TACTACTACA GCTGCCATGT CTGCGGCTTC 
CAGACAGAGT TCAATGCTCA GTTTGTCAGT CATATGTCTC TTCATGTGGA CAAAGAGCAG 
TGGATGTTTT CTCTCTGCTG TAGCACCTGT GAGTATGTGG GTGTGGACGA GGCCGAGATG 
AAAGCTCACA TCAGTGCAGG GCACGCAGGT CTCAATTCTA GAAGCCCTCT CAGTGAGACC 
AAGAGCACTT CCTCCTCTCT GTCAGCCCTC AGCGACTCCC TCAACAGCTC TGAAGGTGGA 
GATGTTGCCC AAGGTAACGA AGAACTAAAA AGCCTTTTGG CTCCGCCTTC ATCTGTGGGA 
AGCCAATCAA GCTTAGGCCC AGGAGCAGAG GAAAAAGCAG AGAAAGGCTT TGAATGCGTC 
TTCTGCAACT TTGTGTGTAA AACCCGACCC ATGTACGAGC GGCACCTACA GATCCACCTG 
ATCACGCGCA TGTTTGAGTG TGACGTCTGC CACAAGTTCA TGAAGACGCC CGAGCAGCTC 
CTGGAGCACA AGAAGTGCCA CACCGTCCCC GCTGGAGGGC TCAAGGTTTG TGAAGCGCTT 
TGGGGCCCTG AGCAGATGAA AGGTGCTATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf827 ( XM_005159979.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160036.1 Danio rerio zinc finger protein 827 (znf827), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa05910
Clone ID Related Accession (Same CDS sequence)	XM_005159980.4 , XM_005159979.3 , XM_005159979.4 , XM_005159980.3
Accession Version	XM_005159979.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	zinc finger protein 827 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159979.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GGAAACAGGA GCAACCCAAG CGCCTGCCTT CACATGTGGA CACAGTAGAG 
GATGGAGGGC CAGAGAGTGG CTCAGTAGAG GAGGACAGCT GGTTTGGGAA TACCTCCGAC 
AGCCCCTCGG AATCGTCCTA CGGTGATGTG CAAGATGAGC TTCGGGTTTC TCCAGACAAC 
GTTCCCCGAC CGGACCACGA CACCTCAGCT GAGAGCTCCC TGGGCTGCCC GACGCCAGTT 
CAGCGGGCCT CTCTGGAGCT TCTGGGCCTG GACAGTGGGG TCCTTTCGAC CCAGGACACG 
CTTTCCTCTG TGGTGTCCTC TCTTTATGGA GACTCTAGCC AGGCTCTGGG TAAGCCACTC 
AGCAGTAATC TGCGCCGCCT CCTGGAGGCT GGCTCACTGA AGCTTGAGGG AGGGGAGCTG 
GCTGGACGTG GAACGGGTAG AGGACAAGAG TCACCCCCGG TCAGCCTGGT ACTATCTCCG 
TCTTCACATC ACGCACAGCA GCTGAGTGCC CTGGCGAGGA GGTTGGCAAA TAGCAGCAAC 
AACAGCGGAA ATTCAAACTC TGCGTCACCT GCATCTGTAG CAACTGTCAA GCAGGAGCCC 
TGTGACCCAT ACGGCCCCAG CAACGGCCCT AACTCAGGCA GCAGTGGCTT TGTATGGGTA 
GGCGTCGCAG ATAAATGGCC ATCGACAAGT TGCTCAGCAC CCAGTGGCAG TAGCCTCTCA 
CCAGACTCAG CAATTCAGAA GTTAAAGGCA GCCGCCAACG CCGTGCTCCA AGACAAGAAC 
GCGGTGGCCT CCTCCACCTC GGCATCTTCA TCCTCCTCGT CCTCCTCCTC ATCCTCTTCA 
GTCACCTCCT CCTCAATGGC AGCCCTGGGC ACTCGCTCTG ATGACGCAGT GCGCTTTGAA 
CCATTCGCTT CACCCTTTAG CCCGCAGTCA GCCAGCTCTA CGCTTGCTGC CCTCTCAAAG 
AAAGTGTCTG AAAGAAGTCA GCAGCAGGTG GGCTCATCTG AGCATCAACC CTCAGCTGGC 
TCCTTTCTCT CTCTGGTTTC CATGACCTCC TCAGCAGCTC TGCTAAAGGA GGTGGCCGCA 
CGAGCAGCAG GGAATCTTCT TGCTGATAAG AAAGAGCCGG CGTTAGTACT GGGAGCCTCC 
GAAGATGTCA AGCCCCTGTT AGACAGGAAC CAGAAGGTGC CCACCCCAAC CCAGGCAATG 
GATCTTCTGC TGCCAACAGT CCCCAAGGGA AGGGCCAAGC CCAGCAGTCA AGCAGGTTCT 
CCTGAGGATG GAGGAAAGCC CTTTCAGTGT CCGGTATGCG GTCTGGTCAT CAAGAGAAAG 
AGCTACTGGA AGAGACATAT GGTGATCCAT ACAGGCTTAA AAAGCCACCA GTGTCCCCTC 
TGTCCCTTTC GCTGTGCTCG TAAAGACAAT CTCAAGTCCC ACATGAAGGT CCATCAGCAC 
CAAGACCGAG GGGAGACGTT CCAGTGTGAG CTCTGCCCCT TCACATCCTC CCGCCACTTT 
AGCCTCAAGC TGCACATGCG TTGCCATCAG CACTTCCCTC GTCCCGACCT CAAAGTCAAA 
GAAGAACCCG GCACAGACAC CGAGGAGGGC GAGGGCTTGC TCATGGGGGA CGGTGGAGCG 
GCTGGTGATC AGGCCATGAA CACAGATGCT TCTACGTCTC CCACTGTGAG CCAACCCGAC 
GCTCGTTTGC TGGCCTCTCC TGCAGAACCC TCAAACCACG TCCAGATCAA GGAGGAACCA 
CAGGAGAGGG ATGTGTCTGT GATGTCTCCC TTTGCCCTGT GTAGGGAGAG GCCGAGCAGC 
AGCAGCAGTT CTCTGGATCT GTCCGGGCTT AAATCTAGTC CACCGGGGCC CGGGCCTACA 
GCTGCTTCAC TCTTCAGCCC AGATATCACC ACCAAGACGG CTACAGACCT CCTCATAAAG 
CTCTCGGCAG CGAACCAGAA GGAGGCCCTA AAGGGTCCAT ACTGTGTCAA GGAAGAGCCC 
CAGGCGGAGG AGCCCCTGAA CTCTCAGAGG GGACCCAGCT ACAGCTTCTG CCAAGAAGGA 
CCTCCAGGAG CCACCAAACC AAGAGAGGAG GGGAGCCCGA TGTTGGGCAA GAGCAGCCTT 
CTCAGCCAGG ACATCAACGT CAAAGTGGCC TCTGAATTGC TTATGAAGCT CTCAGAGAAC 
AACAAAGACG CTTGCGTCCA GAATGTTATT GTGAAGGCGG AGCCTATGGA AGTGGATCCA 
CCCCTTGATC TGGCCTCACC CTCCTCCCAT CTACTGGGCT TTAGCACTTC AGGACCATGT 
GAAAAGAAGG AACCAATGGT CAACCTCCCA GATCATTTGC CCCGCCCCCA GAATGACCTC 
TTCTCGCAGG ACATATCGGT CAAAATGGCC TCCGAGCTGC TTTTCAAACT CTCTGAAAAA 
GTAAGTAAAG CTAATGAAAA CAAAGACAAT GCATCATTCG GTATACACAG CCCTTTCCTT 
GATGACCGCT TTAGACAATC ACCTTTCAGC ACTCGCTCCA AGAGCTCTTC CCCAGCTGAA 
GCCGGATCTT CAAGCAGGGT TCCCCAGCAA GATCCAGAGA AGGTGAGTAG TGAGCCAGGA 
AATGGACTTT CCCAATGGAG ACTCAACGAA CAGCTCTTTC CCTGCCCTGT GTGTGGCAAA 
GTGTTTGGTC GCCAACAGAC TCTCTCCAGG CATCTCTCCT TACACACAGA AGAGAGGAAA 
TACAAGTGTC ATTTGTGTCC ATATGCAGCT AAGTGCCGAG CCAACCTCAA CCAGCACCTC 
ACCATCCACT CAGTCAAACT GGTGAACACA GATGTCGAGC AGATTGTTAC CGCTGTTACC 
AACGATGGCC CAGAGCTCAA AAACTGCCCC TACTACTACA GCTGCCATGT CTGCGGCTTC 
CAGACAGAGT TCAATGCTCA GTTTGTCAGT CATATGTCTC TTCATGTGGA CAAAGAGCAG 
TGGATGTTTT CTCTCTGCTG TAGCACCTGT GAGTATGTGG GTGTGGACGA GGCCGAGATG 
AAAGCTCACA TCAGTGCAGG GCACGCAGGT CTCAATTCTA GAAGCCCTCT CAGTGAGACC 
AAGAGCACTT CCTCCTCTCT GTCAGCCCTC AGCGACTCCC TCAACAGCTC TGAAGGTGGA 
GATGTTGCCC AAGGTAACGA AGAACTAAAA AGCCTTTTGG CTCCGCCTTC ATCTGTGGGA 
AGCCAATCAA GCTTAGGCCC AGGAGCAGAG GAAAAAGCAG AGAAAGGCTT TGAATGCGTC 
TTCTGCAACT TTGTGTGTAA AACCCGACCC ATGTACGAGC GGCACCTACA GATCCACCTG 
ATCACGCGCA TGTTTGAGTG TGACGTCTGC CACAAGTTCA TGAAGACGCC CGAGCAGCTC 
CTGGAGCACA AGAAGTGCCA CACCGTCCCC GCTGGAGGGC TCAAGGTTTG TGAAGCGCTT 
TGGGGCCCTG AGCAGATGAA AGGTGCTATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160036.1
CDS	858..4310
Translation	MPRRKQEQPKRLPSHVDTVEDGGPESGSVEEDSWFGNTSDSPSESSYGDVQDELRVSPDNVPRPDHDTSAESSLGCPTPVQRASLELLGLDSGVLSTQDTLSSVVSSLYGDSSQALGKPLSSNLRRLLEAGSLKLEGGELAGRGTGRGQESPPVSLVLSPSSHHAQQLSALARRLANSSNNSGNSNSASPASVATVKQEPCDPYGPSNGPNSGSSGFVWVGVADKWPSTSCSAPSGSSLSPDSAIQKLKAAANAVLQDKNAVASSTSASSSSSSSSSSSSVTSSSMAALGTRSDDAVRFEPFASPFSPQSASSTLAALSKKVSERSQQQVGSSEHQPSAGSFLSLVSMTSSAALLKEVAARAAGNLLADKKEPALVLGASEDVKPLLDRNQKVPTPTQAMDLLLPTVPKGRAKPSSQAGSPEDGGKPFQCPVCGLVIKRKSYWKRHMVIHTGLKSHQCPLCPFRCARKDNLKSHMKVHQHQDRGETFQCELCPFTSSRHFSLKLHMRCHQHFPRPDLKVKEEPGTDTEEGEGLLMGDGGAAGDQAMNTDASTSPTVSQPDARLLASPAEPSNHVQIKEEPQERDVSVMSPFALCRERPSSSSSSLDLSGLKSSPPGPGPTAASLFSPDITTKTATDLLIKLSAANQKEALKGPYCVKEEPQAEEPLNSQRGPSYSFCQEGPPGATKPREEGSPMLGKSSLLSQDINVKVASELLMKLSENNKDACVQNVIVKAEPMEVDPPLDLASPSSHLLGFSTSGPCEKKEPMVNLPDHLPRPQNDLFSQDISVKMASELLFKLSEKVSKANENKDNASFGIHSPFLDDRFRQSPFSTRSKSSSPAEAGSSSRVPQQDPEKVSSEPGNGLSQWRLNEQLFPCPVCGKVFGRQQTLSRHLSLHTEERKYKCHLCPYAAKCRANLNQHLTIHSVKLVNTDVEQIVTAVTNDGPELKNCPYYYSCHVCGFQTEFNAQFVSHMSLHVDKEQWMFSLCCSTCEYVGVDEAEMKAHISAGHAGLNSRSPLSETKSTSSSLSALSDSLNSSEGGDVAQGNEELKSLLAPPSSVGSQSSLGPGAEEKAEKGFECVFCNFVCKTRPMYERHLQIHLITRMFECDVCHKFMKTPEQLLEHKKCHTVPAGGLKVCEALWGPEQMKGAI

Target ORF information:

RefSeq Version	XM_005159979.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio zinc finger protein 827 (znf827), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159979.3

ORF Insert Sequence:

ATGCCAAGGA GGAAACAGGA GCAACCCAAG CGCCTGCCTT CACATGTGGA CACAGTAGAG 
GATGGAGGGC CAGAGAGTGG CTCAGTAGAG GAGGACAGCT GGTTTGGGAA TACCTCCGAC 
AGCCCCTCGG AATCGTCCTA CGGTGATGTG CAAGATGAGC TTCGGGTTTC TCCAGACAAC 
GTTCCCCGAC CGGACCACGA CACCTCAGCT GAGAGCTCCC TGGGCTGCCC GACGCCAGTT 
CAGCGGGCCT CTCTGGAGCT TCTGGGCCTG GACAGTGGGG TCCTTTCGAC CCAGGACACG 
CTTTCCTCTG TGGTGTCCTC TCTTTATGGA GACTCTAGCC AGGCTCTGGG TAAGCCACTC 
AGCAGTAATC TGCGCCGCCT CCTGGAGGCT GGCTCACTGA AGCTTGAGGG AGGGGAGCTG 
GCTGGACGTG GAACGGGTAG AGGACAAGAG TCACCCCCGG TCAGCCTGGT ACTATCTCCG 
TCTTCACATC ACGCACAGCA GCTGAGTGCC CTGGCGAGGA GGTTGGCAAA TAGCAGCAAC 
AACAGCGGAA ATTCAAACTC TGCGTCACCT GCATCTGTAG CAACTGTCAA GCAGGAGCCC 
TGTGACCCAT ACGGCCCCAG CAACGGCCCT AACTCAGGCA GCAGTGGCTT TGTATGGGTA 
GGCGTCGCAG ATAAATGGCC ATCGACAAGT TGCTCAGCAC CCAGTGGCAG TAGCCTCTCA 
CCAGACTCAG CAATTCAGAA GTTAAAGGCA GCCGCCAACG CCGTGCTCCA AGACAAGAAC 
GCGGTGGCCT CCTCCACCTC GGCATCTTCA TCCTCCTCGT CCTCCTCCTC ATCCTCTTCA 
GTCACCTCCT CCTCAATGGC AGCCCTGGGC ACTCGCTCTG ATGACGCAGT GCGCTTTGAA 
CCATTCGCTT CACCCTTTAG CCCGCAGTCA GCCAGCTCTA CGCTTGCTGC CCTCTCAAAG 
AAAGTGTCTG AAAGAAGTCA GCAGCAGGTG GGCTCATCTG AGCATCAACC CTCAGCTGGC 
TCCTTTCTCT CTCTGGTTTC CATGACCTCC TCAGCAGCTC TGCTAAAGGA GGTGGCCGCA 
CGAGCAGCAG GGAATCTTCT TGCTGATAAG AAAGAGCCGG CGTTAGTACT GGGAGCCTCC 
GAAGATGTCA AGCCCCTGTT AGACAGGAAC CAGAAGGTGC CCACCCCAAC CCAGGCAATG 
GATCTTCTGC TGCCAACAGT CCCCAAGGGA AGGGCCAAGC CCAGCAGTCA AGCAGGTTCT 
CCTGAGGATG GAGGAAAGCC CTTTCAGTGT CCGGTATGCG GTCTGGTCAT CAAGAGAAAG 
AGCTACTGGA AGAGACATAT GGTGATCCAT ACAGGCTTAA AAAGCCACCA GTGTCCCCTC 
TGTCCCTTTC GCTGTGCTCG TAAAGACAAT CTCAAGTCCC ACATGAAGGT CCATCAGCAC 
CAAGACCGAG GGGAGACGTT CCAGTGTGAG CTCTGCCCCT TCACATCCTC CCGCCACTTT 
AGCCTCAAGC TGCACATGCG TTGCCATCAG CACTTCCCTC GTCCCGACCT CAAAGTCAAA 
GAAGAACCCG GCACAGACAC CGAGGAGGGC GAGGGCTTGC TCATGGGGGA CGGTGGAGCG 
GCTGGTGATC AGGCCATGAA CACAGATGCT TCTACGTCTC CCACTGTGAG CCAACCCGAC 
GCTCGTTTGC TGGCCTCTCC TGCAGAACCC TCAAACCACG TCCAGATCAA GGAGGAACCA 
CAGGAGAGGG ATGTGTCTGT GATGTCTCCC TTTGCCCTGT GTAGGGAGAG GCCGAGCAGC 
AGCAGCAGTT CTCTGGATCT GTCCGGGCTT AAATCTAGTC CACCGGGGCC CGGGCCTACA 
GCTGCTTCAC TCTTCAGCCC AGATATCACC ACCAAGACGG CTACAGACCT CCTCATAAAG 
CTCTCGGCAG CGAACCAGAA GGAGGCCCTA AAGGGTCCAT ACTGTGTCAA GGAAGAGCCC 
CAGGCGGAGG AGCCCCTGAA CTCTCAGAGG GGACCCAGCT ACAGCTTCTG CCAAGAAGGA 
CCTCCAGGAG CCACCAAACC AAGAGAGGAG GGGAGCCCGA TGTTGGGCAA GAGCAGCCTT 
CTCAGCCAGG ACATCAACGT CAAAGTGGCC TCTGAATTGC TTATGAAGCT CTCAGAGAAC 
AACAAAGACG CTTGCGTCCA GAATGTTATT GTGAAGGCGG AGCCTATGGA AGTGGATCCA 
CCCCTTGATC TGGCCTCACC CTCCTCCCAT CTACTGGGCT TTAGCACTTC AGGACCATGT 
GAAAAGAAGG AACCAATGGT CAACCTCCCA GATCATTTGC CCCGCCCCCA GAATGACCTC 
TTCTCGCAGG ACATATCGGT CAAAATGGCC TCCGAGCTGC TTTTCAAACT CTCTGAAAAA 
GTAAGTAAAG CTAATGAAAA CAAAGACAAT GCATCATTCG GTATACACAG CCCTTTCCTT 
GATGACCGCT TTAGACAATC ACCTTTCAGC ACTCGCTCCA AGAGCTCTTC CCCAGCTGAA 
GCCGGATCTT CAAGCAGGGT TCCCCAGCAA GATCCAGAGA AGGTGAGTAG TGAGCCAGGA 
AATGGACTTT CCCAATGGAG ACTCAACGAA CAGCTCTTTC CCTGCCCTGT GTGTGGCAAA 
GTGTTTGGTC GCCAACAGAC TCTCTCCAGG CATCTCTCCT TACACACAGA AGAGAGGAAA 
TACAAGTGTC ATTTGTGTCC ATATGCAGCT AAGTGCCGAG CCAACCTCAA CCAGCACCTC 
ACCATCCACT CAGTCAAACT GGTGAACACA GATGTCGAGC AGATTGTTAC CGCTGTTACC 
AACGATGGCC CAGAGCTCAA AAACTGCCCC TACTACTACA GCTGCCATGT CTGCGGCTTC 
CAGACAGAGT TCAATGCTCA GTTTGTCAGT CATATGTCTC TTCATGTGGA CAAAGAGCAG 
TGGATGTTTT CTCTCTGCTG TAGCACCTGT GAGTATGTGG GTGTGGACGA GGCCGAGATG 
AAAGCTCACA TCAGTGCAGG GCACGCAGGT CTCAATTCTA GAAGCCCTCT CAGTGAGACC 
AAGAGCACTT CCTCCTCTCT GTCAGCCCTC AGCGACTCCC TCAACAGCTC TGAAGGTGGA 
GATGTTGCCC AAGGTAACGA AGAACTAAAA AGCCTTTTGG CTCCGCCTTC ATCTGTGGGA 
AGCCAATCAA GCTTAGGCCC AGGAGCAGAG GAAAAAGCAG AGAAAGGCTT TGAATGCGTC 
TTCTGCAACT TTGTGTGTAA AACCCGACCC ATGTACGAGC GGCACCTACA GATCCACCTG 
ATCACGCGCA TGTTTGAGTG TGACGTCTGC CACAAGTTCA TGAAGACGCC CGAGCAGCTC 
CTGGAGCACA AGAAGTGCCA CACCGTCCCC GCTGGAGGGC TCAAGGTTTG TGAAGCGCTT 
TGGGGCCCTG AGCAGATGAA AGGTGCTATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf827 ( XM_005159979.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160036.1 Danio rerio zinc finger protein 827 (znf827), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa05910
Clone ID Related Accession (Same CDS sequence)	XM_005159980.4 , XM_005159979.3 , XM_005159979.4 , XM_005159980.3
Accession Version	XM_005159979.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	zinc finger protein 827 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159979.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GGAAACAGGA GCAACCCAAG CGCCTGCCTT CACATGTGGA CACAGTAGAG 
GATGGAGGGC CAGAGAGTGG CTCAGTAGAG GAGGACAGCT GGTTTGGGAA TACCTCCGAC 
AGCCCCTCGG AATCGTCCTA CGGTGATGTG CAAGATGAGC TTCGGGTTTC TCCAGACAAC 
GTTCCCCGAC CGGACCACGA CACCTCAGCT GAGAGCTCCC TGGGCTGCCC GACGCCAGTT 
CAGCGGGCCT CTCTGGAGCT TCTGGGCCTG GACAGTGGGG TCCTTTCGAC CCAGGACACG 
CTTTCCTCTG TGGTGTCCTC TCTTTATGGA GACTCTAGCC AGGCTCTGGG TAAGCCACTC 
AGCAGTAATC TGCGCCGCCT CCTGGAGGCT GGCTCACTGA AGCTTGAGGG AGGGGAGCTG 
GCTGGACGTG GAACGGGTAG AGGACAAGAG TCACCCCCGG TCAGCCTGGT ACTATCTCCG 
TCTTCACATC ACGCACAGCA GCTGAGTGCC CTGGCGAGGA GGTTGGCAAA TAGCAGCAAC 
AACAGCGGAA ATTCAAACTC TGCGTCACCT GCATCTGTAG CAACTGTCAA GCAGGAGCCC 
TGTGACCCAT ACGGCCCCAG CAACGGCCCT AACTCAGGCA GCAGTGGCTT TGTATGGGTA 
GGCGTCGCAG ATAAATGGCC ATCGACAAGT TGCTCAGCAC CCAGTGGCAG TAGCCTCTCA 
CCAGACTCAG CAATTCAGAA GTTAAAGGCA GCCGCCAACG CCGTGCTCCA AGACAAGAAC 
GCGGTGGCCT CCTCCACCTC GGCATCTTCA TCCTCCTCGT CCTCCTCCTC ATCCTCTTCA 
GTCACCTCCT CCTCAATGGC AGCCCTGGGC ACTCGCTCTG ATGACGCAGT GCGCTTTGAA 
CCATTCGCTT CACCCTTTAG CCCGCAGTCA GCCAGCTCTA CGCTTGCTGC CCTCTCAAAG 
AAAGTGTCTG AAAGAAGTCA GCAGCAGGTG GGCTCATCTG AGCATCAACC CTCAGCTGGC 
TCCTTTCTCT CTCTGGTTTC CATGACCTCC TCAGCAGCTC TGCTAAAGGA GGTGGCCGCA 
CGAGCAGCAG GGAATCTTCT TGCTGATAAG AAAGAGCCGG CGTTAGTACT GGGAGCCTCC 
GAAGATGTCA AGCCCCTGTT AGACAGGAAC CAGAAGGTGC CCACCCCAAC CCAGGCAATG 
GATCTTCTGC TGCCAACAGT CCCCAAGGGA AGGGCCAAGC CCAGCAGTCA AGCAGGTTCT 
CCTGAGGATG GAGGAAAGCC CTTTCAGTGT CCGGTATGCG GTCTGGTCAT CAAGAGAAAG 
AGCTACTGGA AGAGACATAT GGTGATCCAT ACAGGCTTAA AAAGCCACCA GTGTCCCCTC 
TGTCCCTTTC GCTGTGCTCG TAAAGACAAT CTCAAGTCCC ACATGAAGGT CCATCAGCAC 
CAAGACCGAG GGGAGACGTT CCAGTGTGAG CTCTGCCCCT TCACATCCTC CCGCCACTTT 
AGCCTCAAGC TGCACATGCG TTGCCATCAG CACTTCCCTC GTCCCGACCT CAAAGTCAAA 
GAAGAACCCG GCACAGACAC CGAGGAGGGC GAGGGCTTGC TCATGGGGGA CGGTGGAGCG 
GCTGGTGATC AGGCCATGAA CACAGATGCT TCTACGTCTC CCACTGTGAG CCAACCCGAC 
GCTCGTTTGC TGGCCTCTCC TGCAGAACCC TCAAACCACG TCCAGATCAA GGAGGAACCA 
CAGGAGAGGG ATGTGTCTGT GATGTCTCCC TTTGCCCTGT GTAGGGAGAG GCCGAGCAGC 
AGCAGCAGTT CTCTGGATCT GTCCGGGCTT AAATCTAGTC CACCGGGGCC CGGGCCTACA 
GCTGCTTCAC TCTTCAGCCC AGATATCACC ACCAAGACGG CTACAGACCT CCTCATAAAG 
CTCTCGGCAG CGAACCAGAA GGAGGCCCTA AAGGGTCCAT ACTGTGTCAA GGAAGAGCCC 
CAGGCGGAGG AGCCCCTGAA CTCTCAGAGG GGACCCAGCT ACAGCTTCTG CCAAGAAGGA 
CCTCCAGGAG CCACCAAACC AAGAGAGGAG GGGAGCCCGA TGTTGGGCAA GAGCAGCCTT 
CTCAGCCAGG ACATCAACGT CAAAGTGGCC TCTGAATTGC TTATGAAGCT CTCAGAGAAC 
AACAAAGACG CTTGCGTCCA GAATGTTATT GTGAAGGCGG AGCCTATGGA AGTGGATCCA 
CCCCTTGATC TGGCCTCACC CTCCTCCCAT CTACTGGGCT TTAGCACTTC AGGACCATGT 
GAAAAGAAGG AACCAATGGT CAACCTCCCA GATCATTTGC CCCGCCCCCA GAATGACCTC 
TTCTCGCAGG ACATATCGGT CAAAATGGCC TCCGAGCTGC TTTTCAAACT CTCTGAAAAA 
GTAAGTAAAG CTAATGAAAA CAAAGACAAT GCATCATTCG GTATACACAG CCCTTTCCTT 
GATGACCGCT TTAGACAATC ACCTTTCAGC ACTCGCTCCA AGAGCTCTTC CCCAGCTGAA 
GCCGGATCTT CAAGCAGGGT TCCCCAGCAA GATCCAGAGA AGGTGAGTAG TGAGCCAGGA 
AATGGACTTT CCCAATGGAG ACTCAACGAA CAGCTCTTTC CCTGCCCTGT GTGTGGCAAA 
GTGTTTGGTC GCCAACAGAC TCTCTCCAGG CATCTCTCCT TACACACAGA AGAGAGGAAA 
TACAAGTGTC ATTTGTGTCC ATATGCAGCT AAGTGCCGAG CCAACCTCAA CCAGCACCTC 
ACCATCCACT CAGTCAAACT GGTGAACACA GATGTCGAGC AGATTGTTAC CGCTGTTACC 
AACGATGGCC CAGAGCTCAA AAACTGCCCC TACTACTACA GCTGCCATGT CTGCGGCTTC 
CAGACAGAGT TCAATGCTCA GTTTGTCAGT CATATGTCTC TTCATGTGGA CAAAGAGCAG 
TGGATGTTTT CTCTCTGCTG TAGCACCTGT GAGTATGTGG GTGTGGACGA GGCCGAGATG 
AAAGCTCACA TCAGTGCAGG GCACGCAGGT CTCAATTCTA GAAGCCCTCT CAGTGAGACC 
AAGAGCACTT CCTCCTCTCT GTCAGCCCTC AGCGACTCCC TCAACAGCTC TGAAGGTGGA 
GATGTTGCCC AAGGTAACGA AGAACTAAAA AGCCTTTTGG CTCCGCCTTC ATCTGTGGGA 
AGCCAATCAA GCTTAGGCCC AGGAGCAGAG GAAAAAGCAG AGAAAGGCTT TGAATGCGTC 
TTCTGCAACT TTGTGTGTAA AACCCGACCC ATGTACGAGC GGCACCTACA GATCCACCTG 
ATCACGCGCA TGTTTGAGTG TGACGTCTGC CACAAGTTCA TGAAGACGCC CGAGCAGCTC 
CTGGAGCACA AGAAGTGCCA CACCGTCCCC GCTGGAGGGC TCAAGGTTTG TGAAGCGCTT 
TGGGGCCCTG AGCAGATGAA AGGTGCTATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160036.1
CDS	415..3867
Translation	MPRRKQEQPKRLPSHVDTVEDGGPESGSVEEDSWFGNTSDSPSESSYGDVQDELRVSPDNVPRPDHDTSAESSLGCPTPVQRASLELLGLDSGVLSTQDTLSSVVSSLYGDSSQALGKPLSSNLRRLLEAGSLKLEGGELAGRGTGRGQESPPVSLVLSPSSHHAQQLSALARRLANSSNNSGNSNSASPASVATVKQEPCDPYGPSNGPNSGSSGFVWVGVADKWPSTSCSAPSGSSLSPDSAIQKLKAAANAVLQDKNAVASSTSASSSSSSSSSSSSVTSSSMAALGTRSDDAVRFEPFASPFSPQSASSTLAALSKKVSERSQQQVGSSEHQPSAGSFLSLVSMTSSAALLKEVAARAAGNLLADKKEPALVLGASEDVKPLLDRNQKVPTPTQAMDLLLPTVPKGRAKPSSQAGSPEDGGKPFQCPVCGLVIKRKSYWKRHMVIHTGLKSHQCPLCPFRCARKDNLKSHMKVHQHQDRGETFQCELCPFTSSRHFSLKLHMRCHQHFPRPDLKVKEEPGTDTEEGEGLLMGDGGAAGDQAMNTDASTSPTVSQPDARLLASPAEPSNHVQIKEEPQERDVSVMSPFALCRERPSSSSSSLDLSGLKSSPPGPGPTAASLFSPDITTKTATDLLIKLSAANQKEALKGPYCVKEEPQAEEPLNSQRGPSYSFCQEGPPGATKPREEGSPMLGKSSLLSQDINVKVASELLMKLSENNKDACVQNVIVKAEPMEVDPPLDLASPSSHLLGFSTSGPCEKKEPMVNLPDHLPRPQNDLFSQDISVKMASELLFKLSEKVSKANENKDNASFGIHSPFLDDRFRQSPFSTRSKSSSPAEAGSSSRVPQQDPEKVSSEPGNGLSQWRLNEQLFPCPVCGKVFGRQQTLSRHLSLHTEERKYKCHLCPYAAKCRANLNQHLTIHSVKLVNTDVEQIVTAVTNDGPELKNCPYYYSCHVCGFQTEFNAQFVSHMSLHVDKEQWMFSLCCSTCEYVGVDEAEMKAHISAGHAGLNSRSPLSETKSTSSSLSALSDSLNSSEGGDVAQGNEELKSLLAPPSSVGSQSSLGPGAEEKAEKGFECVFCNFVCKTRPMYERHLQIHLITRMFECDVCHKFMKTPEQLLEHKKCHTVPAGGLKVCEALWGPEQMKGAI

Target ORF information:

RefSeq Version	XM_005159979.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zinc finger protein 827 (znf827), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159979.4

ORF Insert Sequence:

ATGCCAAGGA GGAAACAGGA GCAACCCAAG CGCCTGCCTT CACATGTGGA CACAGTAGAG 
GATGGAGGGC CAGAGAGTGG CTCAGTAGAG GAGGACAGCT GGTTTGGGAA TACCTCCGAC 
AGCCCCTCGG AATCGTCCTA CGGTGATGTG CAAGATGAGC TTCGGGTTTC TCCAGACAAC 
GTTCCCCGAC CGGACCACGA CACCTCAGCT GAGAGCTCCC TGGGCTGCCC GACGCCAGTT 
CAGCGGGCCT CTCTGGAGCT TCTGGGCCTG GACAGTGGGG TCCTTTCGAC CCAGGACACG 
CTTTCCTCTG TGGTGTCCTC TCTTTATGGA GACTCTAGCC AGGCTCTGGG TAAGCCACTC 
AGCAGTAATC TGCGCCGCCT CCTGGAGGCT GGCTCACTGA AGCTTGAGGG AGGGGAGCTG 
GCTGGACGTG GAACGGGTAG AGGACAAGAG TCACCCCCGG TCAGCCTGGT ACTATCTCCG 
TCTTCACATC ACGCACAGCA GCTGAGTGCC CTGGCGAGGA GGTTGGCAAA TAGCAGCAAC 
AACAGCGGAA ATTCAAACTC TGCGTCACCT GCATCTGTAG CAACTGTCAA GCAGGAGCCC 
TGTGACCCAT ACGGCCCCAG CAACGGCCCT AACTCAGGCA GCAGTGGCTT TGTATGGGTA 
GGCGTCGCAG ATAAATGGCC ATCGACAAGT TGCTCAGCAC CCAGTGGCAG TAGCCTCTCA 
CCAGACTCAG CAATTCAGAA GTTAAAGGCA GCCGCCAACG CCGTGCTCCA AGACAAGAAC 
GCGGTGGCCT CCTCCACCTC GGCATCTTCA TCCTCCTCGT CCTCCTCCTC ATCCTCTTCA 
GTCACCTCCT CCTCAATGGC AGCCCTGGGC ACTCGCTCTG ATGACGCAGT GCGCTTTGAA 
CCATTCGCTT CACCCTTTAG CCCGCAGTCA GCCAGCTCTA CGCTTGCTGC CCTCTCAAAG 
AAAGTGTCTG AAAGAAGTCA GCAGCAGGTG GGCTCATCTG AGCATCAACC CTCAGCTGGC 
TCCTTTCTCT CTCTGGTTTC CATGACCTCC TCAGCAGCTC TGCTAAAGGA GGTGGCCGCA 
CGAGCAGCAG GGAATCTTCT TGCTGATAAG AAAGAGCCGG CGTTAGTACT GGGAGCCTCC 
GAAGATGTCA AGCCCCTGTT AGACAGGAAC CAGAAGGTGC CCACCCCAAC CCAGGCAATG 
GATCTTCTGC TGCCAACAGT CCCCAAGGGA AGGGCCAAGC CCAGCAGTCA AGCAGGTTCT 
CCTGAGGATG GAGGAAAGCC CTTTCAGTGT CCGGTATGCG GTCTGGTCAT CAAGAGAAAG 
AGCTACTGGA AGAGACATAT GGTGATCCAT ACAGGCTTAA AAAGCCACCA GTGTCCCCTC 
TGTCCCTTTC GCTGTGCTCG TAAAGACAAT CTCAAGTCCC ACATGAAGGT CCATCAGCAC 
CAAGACCGAG GGGAGACGTT CCAGTGTGAG CTCTGCCCCT TCACATCCTC CCGCCACTTT 
AGCCTCAAGC TGCACATGCG TTGCCATCAG CACTTCCCTC GTCCCGACCT CAAAGTCAAA 
GAAGAACCCG GCACAGACAC CGAGGAGGGC GAGGGCTTGC TCATGGGGGA CGGTGGAGCG 
GCTGGTGATC AGGCCATGAA CACAGATGCT TCTACGTCTC CCACTGTGAG CCAACCCGAC 
GCTCGTTTGC TGGCCTCTCC TGCAGAACCC TCAAACCACG TCCAGATCAA GGAGGAACCA 
CAGGAGAGGG ATGTGTCTGT GATGTCTCCC TTTGCCCTGT GTAGGGAGAG GCCGAGCAGC 
AGCAGCAGTT CTCTGGATCT GTCCGGGCTT AAATCTAGTC CACCGGGGCC CGGGCCTACA 
GCTGCTTCAC TCTTCAGCCC AGATATCACC ACCAAGACGG CTACAGACCT CCTCATAAAG 
CTCTCGGCAG CGAACCAGAA GGAGGCCCTA AAGGGTCCAT ACTGTGTCAA GGAAGAGCCC 
CAGGCGGAGG AGCCCCTGAA CTCTCAGAGG GGACCCAGCT ACAGCTTCTG CCAAGAAGGA 
CCTCCAGGAG CCACCAAACC AAGAGAGGAG GGGAGCCCGA TGTTGGGCAA GAGCAGCCTT 
CTCAGCCAGG ACATCAACGT CAAAGTGGCC TCTGAATTGC TTATGAAGCT CTCAGAGAAC 
AACAAAGACG CTTGCGTCCA GAATGTTATT GTGAAGGCGG AGCCTATGGA AGTGGATCCA 
CCCCTTGATC TGGCCTCACC CTCCTCCCAT CTACTGGGCT TTAGCACTTC AGGACCATGT 
GAAAAGAAGG AACCAATGGT CAACCTCCCA GATCATTTGC CCCGCCCCCA GAATGACCTC 
TTCTCGCAGG ACATATCGGT CAAAATGGCC TCCGAGCTGC TTTTCAAACT CTCTGAAAAA 
GTAAGTAAAG CTAATGAAAA CAAAGACAAT GCATCATTCG GTATACACAG CCCTTTCCTT 
GATGACCGCT TTAGACAATC ACCTTTCAGC ACTCGCTCCA AGAGCTCTTC CCCAGCTGAA 
GCCGGATCTT CAAGCAGGGT TCCCCAGCAA GATCCAGAGA AGGTGAGTAG TGAGCCAGGA 
AATGGACTTT CCCAATGGAG ACTCAACGAA CAGCTCTTTC CCTGCCCTGT GTGTGGCAAA 
GTGTTTGGTC GCCAACAGAC TCTCTCCAGG CATCTCTCCT TACACACAGA AGAGAGGAAA 
TACAAGTGTC ATTTGTGTCC ATATGCAGCT AAGTGCCGAG CCAACCTCAA CCAGCACCTC 
ACCATCCACT CAGTCAAACT GGTGAACACA GATGTCGAGC AGATTGTTAC CGCTGTTACC 
AACGATGGCC CAGAGCTCAA AAACTGCCCC TACTACTACA GCTGCCATGT CTGCGGCTTC 
CAGACAGAGT TCAATGCTCA GTTTGTCAGT CATATGTCTC TTCATGTGGA CAAAGAGCAG 
TGGATGTTTT CTCTCTGCTG TAGCACCTGT GAGTATGTGG GTGTGGACGA GGCCGAGATG 
AAAGCTCACA TCAGTGCAGG GCACGCAGGT CTCAATTCTA GAAGCCCTCT CAGTGAGACC 
AAGAGCACTT CCTCCTCTCT GTCAGCCCTC AGCGACTCCC TCAACAGCTC TGAAGGTGGA 
GATGTTGCCC AAGGTAACGA AGAACTAAAA AGCCTTTTGG CTCCGCCTTC ATCTGTGGGA 
AGCCAATCAA GCTTAGGCCC AGGAGCAGAG GAAAAAGCAG AGAAAGGCTT TGAATGCGTC 
TTCTGCAACT TTGTGTGTAA AACCCGACCC ATGTACGAGC GGCACCTACA GATCCACCTG 
ATCACGCGCA TGTTTGAGTG TGACGTCTGC CACAAGTTCA TGAAGACGCC CGAGCAGCTC 
CTGGAGCACA AGAAGTGCCA CACCGTCCCC GCTGGAGGGC TCAAGGTTTG TGAAGCGCTT 
TGGGGCCCTG AGCAGATGAA AGGTGCTATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf827 ( XM_005159980.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160037.1 Danio rerio zinc finger protein 827 (znf827), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa05910
Clone ID Related Accession (Same CDS sequence)	XM_005159980.4 , XM_005159979.3 , XM_005159979.4 , XM_005159980.3
Accession Version	XM_005159980.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	zinc finger protein 827 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159980.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GGAAACAGGA GCAACCCAAG CGCCTGCCTT CACATGTGGA CACAGTAGAG 
GATGGAGGGC CAGAGAGTGG CTCAGTAGAG GAGGACAGCT GGTTTGGGAA TACCTCCGAC 
AGCCCCTCGG AATCGTCCTA CGGTGATGTG CAAGATGAGC TTCGGGTTTC TCCAGACAAC 
GTTCCCCGAC CGGACCACGA CACCTCAGCT GAGAGCTCCC TGGGCTGCCC GACGCCAGTT 
CAGCGGGCCT CTCTGGAGCT TCTGGGCCTG GACAGTGGGG TCCTTTCGAC CCAGGACACG 
CTTTCCTCTG TGGTGTCCTC TCTTTATGGA GACTCTAGCC AGGCTCTGGG TAAGCCACTC 
AGCAGTAATC TGCGCCGCCT CCTGGAGGCT GGCTCACTGA AGCTTGAGGG AGGGGAGCTG 
GCTGGACGTG GAACGGGTAG AGGACAAGAG TCACCCCCGG TCAGCCTGGT ACTATCTCCG 
TCTTCACATC ACGCACAGCA GCTGAGTGCC CTGGCGAGGA GGTTGGCAAA TAGCAGCAAC 
AACAGCGGAA ATTCAAACTC TGCGTCACCT GCATCTGTAG CAACTGTCAA GCAGGAGCCC 
TGTGACCCAT ACGGCCCCAG CAACGGCCCT AACTCAGGCA GCAGTGGCTT TGTATGGGTA 
GGCGTCGCAG ATAAATGGCC ATCGACAAGT TGCTCAGCAC CCAGTGGCAG TAGCCTCTCA 
CCAGACTCAG CAATTCAGAA GTTAAAGGCA GCCGCCAACG CCGTGCTCCA AGACAAGAAC 
GCGGTGGCCT CCTCCACCTC GGCATCTTCA TCCTCCTCGT CCTCCTCCTC ATCCTCTTCA 
GTCACCTCCT CCTCAATGGC AGCCCTGGGC ACTCGCTCTG ATGACGCAGT GCGCTTTGAA 
CCATTCGCTT CACCCTTTAG CCCGCAGTCA GCCAGCTCTA CGCTTGCTGC CCTCTCAAAG 
AAAGTGTCTG AAAGAAGTCA GCAGCAGGTG GGCTCATCTG AGCATCAACC CTCAGCTGGC 
TCCTTTCTCT CTCTGGTTTC CATGACCTCC TCAGCAGCTC TGCTAAAGGA GGTGGCCGCA 
CGAGCAGCAG GGAATCTTCT TGCTGATAAG AAAGAGCCGG CGTTAGTACT GGGAGCCTCC 
GAAGATGTCA AGCCCCTGTT AGACAGGAAC CAGAAGGTGC CCACCCCAAC CCAGGCAATG 
GATCTTCTGC TGCCAACAGT CCCCAAGGGA AGGGCCAAGC CCAGCAGTCA AGCAGGTTCT 
CCTGAGGATG GAGGAAAGCC CTTTCAGTGT CCGGTATGCG GTCTGGTCAT CAAGAGAAAG 
AGCTACTGGA AGAGACATAT GGTGATCCAT ACAGGCTTAA AAAGCCACCA GTGTCCCCTC 
TGTCCCTTTC GCTGTGCTCG TAAAGACAAT CTCAAGTCCC ACATGAAGGT CCATCAGCAC 
CAAGACCGAG GGGAGACGTT CCAGTGTGAG CTCTGCCCCT TCACATCCTC CCGCCACTTT 
AGCCTCAAGC TGCACATGCG TTGCCATCAG CACTTCCCTC GTCCCGACCT CAAAGTCAAA 
GAAGAACCCG GCACAGACAC CGAGGAGGGC GAGGGCTTGC TCATGGGGGA CGGTGGAGCG 
GCTGGTGATC AGGCCATGAA CACAGATGCT TCTACGTCTC CCACTGTGAG CCAACCCGAC 
GCTCGTTTGC TGGCCTCTCC TGCAGAACCC TCAAACCACG TCCAGATCAA GGAGGAACCA 
CAGGAGAGGG ATGTGTCTGT GATGTCTCCC TTTGCCCTGT GTAGGGAGAG GCCGAGCAGC 
AGCAGCAGTT CTCTGGATCT GTCCGGGCTT AAATCTAGTC CACCGGGGCC CGGGCCTACA 
GCTGCTTCAC TCTTCAGCCC AGATATCACC ACCAAGACGG CTACAGACCT CCTCATAAAG 
CTCTCGGCAG CGAACCAGAA GGAGGCCCTA AAGGGTCCAT ACTGTGTCAA GGAAGAGCCC 
CAGGCGGAGG AGCCCCTGAA CTCTCAGAGG GGACCCAGCT ACAGCTTCTG CCAAGAAGGA 
CCTCCAGGAG CCACCAAACC AAGAGAGGAG GGGAGCCCGA TGTTGGGCAA GAGCAGCCTT 
CTCAGCCAGG ACATCAACGT CAAAGTGGCC TCTGAATTGC TTATGAAGCT CTCAGAGAAC 
AACAAAGACG CTTGCGTCCA GAATGTTATT GTGAAGGCGG AGCCTATGGA AGTGGATCCA 
CCCCTTGATC TGGCCTCACC CTCCTCCCAT CTACTGGGCT TTAGCACTTC AGGACCATGT 
GAAAAGAAGG AACCAATGGT CAACCTCCCA GATCATTTGC CCCGCCCCCA GAATGACCTC 
TTCTCGCAGG ACATATCGGT CAAAATGGCC TCCGAGCTGC TTTTCAAACT CTCTGAAAAA 
GTAAGTAAAG CTAATGAAAA CAAAGACAAT GCATCATTCG GTATACACAG CCCTTTCCTT 
GATGACCGCT TTAGACAATC ACCTTTCAGC ACTCGCTCCA AGAGCTCTTC CCCAGCTGAA 
GCCGGATCTT CAAGCAGGGT TCCCCAGCAA GATCCAGAGA AGGTGAGTAG TGAGCCAGGA 
AATGGACTTT CCCAATGGAG ACTCAACGAA CAGCTCTTTC CCTGCCCTGT GTGTGGCAAA 
GTGTTTGGTC GCCAACAGAC TCTCTCCAGG CATCTCTCCT TACACACAGA AGAGAGGAAA 
TACAAGTGTC ATTTGTGTCC ATATGCAGCT AAGTGCCGAG CCAACCTCAA CCAGCACCTC 
ACCATCCACT CAGTCAAACT GGTGAACACA GATGTCGAGC AGATTGTTAC CGCTGTTACC 
AACGATGGCC CAGAGCTCAA AAACTGCCCC TACTACTACA GCTGCCATGT CTGCGGCTTC 
CAGACAGAGT TCAATGCTCA GTTTGTCAGT CATATGTCTC TTCATGTGGA CAAAGAGCAG 
TGGATGTTTT CTCTCTGCTG TAGCACCTGT GAGTATGTGG GTGTGGACGA GGCCGAGATG 
AAAGCTCACA TCAGTGCAGG GCACGCAGGT CTCAATTCTA GAAGCCCTCT CAGTGAGACC 
AAGAGCACTT CCTCCTCTCT GTCAGCCCTC AGCGACTCCC TCAACAGCTC TGAAGGTGGA 
GATGTTGCCC AAGGTAACGA AGAACTAAAA AGCCTTTTGG CTCCGCCTTC ATCTGTGGGA 
AGCCAATCAA GCTTAGGCCC AGGAGCAGAG GAAAAAGCAG AGAAAGGCTT TGAATGCGTC 
TTCTGCAACT TTGTGTGTAA AACCCGACCC ATGTACGAGC GGCACCTACA GATCCACCTG 
ATCACGCGCA TGTTTGAGTG TGACGTCTGC CACAAGTTCA TGAAGACGCC CGAGCAGCTC 
CTGGAGCACA AGAAGTGCCA CACCGTCCCC GCTGGAGGGC TCAAGGTTTG TGAAGCGCTT 
TGGGGCCCTG AGCAGATGAA AGGTGCTATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160037.1
CDS	859..4311
Translation	MPRRKQEQPKRLPSHVDTVEDGGPESGSVEEDSWFGNTSDSPSESSYGDVQDELRVSPDNVPRPDHDTSAESSLGCPTPVQRASLELLGLDSGVLSTQDTLSSVVSSLYGDSSQALGKPLSSNLRRLLEAGSLKLEGGELAGRGTGRGQESPPVSLVLSPSSHHAQQLSALARRLANSSNNSGNSNSASPASVATVKQEPCDPYGPSNGPNSGSSGFVWVGVADKWPSTSCSAPSGSSLSPDSAIQKLKAAANAVLQDKNAVASSTSASSSSSSSSSSSSVTSSSMAALGTRSDDAVRFEPFASPFSPQSASSTLAALSKKVSERSQQQVGSSEHQPSAGSFLSLVSMTSSAALLKEVAARAAGNLLADKKEPALVLGASEDVKPLLDRNQKVPTPTQAMDLLLPTVPKGRAKPSSQAGSPEDGGKPFQCPVCGLVIKRKSYWKRHMVIHTGLKSHQCPLCPFRCARKDNLKSHMKVHQHQDRGETFQCELCPFTSSRHFSLKLHMRCHQHFPRPDLKVKEEPGTDTEEGEGLLMGDGGAAGDQAMNTDASTSPTVSQPDARLLASPAEPSNHVQIKEEPQERDVSVMSPFALCRERPSSSSSSLDLSGLKSSPPGPGPTAASLFSPDITTKTATDLLIKLSAANQKEALKGPYCVKEEPQAEEPLNSQRGPSYSFCQEGPPGATKPREEGSPMLGKSSLLSQDINVKVASELLMKLSENNKDACVQNVIVKAEPMEVDPPLDLASPSSHLLGFSTSGPCEKKEPMVNLPDHLPRPQNDLFSQDISVKMASELLFKLSEKVSKANENKDNASFGIHSPFLDDRFRQSPFSTRSKSSSPAEAGSSSRVPQQDPEKVSSEPGNGLSQWRLNEQLFPCPVCGKVFGRQQTLSRHLSLHTEERKYKCHLCPYAAKCRANLNQHLTIHSVKLVNTDVEQIVTAVTNDGPELKNCPYYYSCHVCGFQTEFNAQFVSHMSLHVDKEQWMFSLCCSTCEYVGVDEAEMKAHISAGHAGLNSRSPLSETKSTSSSLSALSDSLNSSEGGDVAQGNEELKSLLAPPSSVGSQSSLGPGAEEKAEKGFECVFCNFVCKTRPMYERHLQIHLITRMFECDVCHKFMKTPEQLLEHKKCHTVPAGGLKVCEALWGPEQMKGAI

Target ORF information:

RefSeq Version	XM_005159980.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio zinc finger protein 827 (znf827), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159980.3

ORF Insert Sequence:

ATGCCAAGGA GGAAACAGGA GCAACCCAAG CGCCTGCCTT CACATGTGGA CACAGTAGAG 
GATGGAGGGC CAGAGAGTGG CTCAGTAGAG GAGGACAGCT GGTTTGGGAA TACCTCCGAC 
AGCCCCTCGG AATCGTCCTA CGGTGATGTG CAAGATGAGC TTCGGGTTTC TCCAGACAAC 
GTTCCCCGAC CGGACCACGA CACCTCAGCT GAGAGCTCCC TGGGCTGCCC GACGCCAGTT 
CAGCGGGCCT CTCTGGAGCT TCTGGGCCTG GACAGTGGGG TCCTTTCGAC CCAGGACACG 
CTTTCCTCTG TGGTGTCCTC TCTTTATGGA GACTCTAGCC AGGCTCTGGG TAAGCCACTC 
AGCAGTAATC TGCGCCGCCT CCTGGAGGCT GGCTCACTGA AGCTTGAGGG AGGGGAGCTG 
GCTGGACGTG GAACGGGTAG AGGACAAGAG TCACCCCCGG TCAGCCTGGT ACTATCTCCG 
TCTTCACATC ACGCACAGCA GCTGAGTGCC CTGGCGAGGA GGTTGGCAAA TAGCAGCAAC 
AACAGCGGAA ATTCAAACTC TGCGTCACCT GCATCTGTAG CAACTGTCAA GCAGGAGCCC 
TGTGACCCAT ACGGCCCCAG CAACGGCCCT AACTCAGGCA GCAGTGGCTT TGTATGGGTA 
GGCGTCGCAG ATAAATGGCC ATCGACAAGT TGCTCAGCAC CCAGTGGCAG TAGCCTCTCA 
CCAGACTCAG CAATTCAGAA GTTAAAGGCA GCCGCCAACG CCGTGCTCCA AGACAAGAAC 
GCGGTGGCCT CCTCCACCTC GGCATCTTCA TCCTCCTCGT CCTCCTCCTC ATCCTCTTCA 
GTCACCTCCT CCTCAATGGC AGCCCTGGGC ACTCGCTCTG ATGACGCAGT GCGCTTTGAA 
CCATTCGCTT CACCCTTTAG CCCGCAGTCA GCCAGCTCTA CGCTTGCTGC CCTCTCAAAG 
AAAGTGTCTG AAAGAAGTCA GCAGCAGGTG GGCTCATCTG AGCATCAACC CTCAGCTGGC 
TCCTTTCTCT CTCTGGTTTC CATGACCTCC TCAGCAGCTC TGCTAAAGGA GGTGGCCGCA 
CGAGCAGCAG GGAATCTTCT TGCTGATAAG AAAGAGCCGG CGTTAGTACT GGGAGCCTCC 
GAAGATGTCA AGCCCCTGTT AGACAGGAAC CAGAAGGTGC CCACCCCAAC CCAGGCAATG 
GATCTTCTGC TGCCAACAGT CCCCAAGGGA AGGGCCAAGC CCAGCAGTCA AGCAGGTTCT 
CCTGAGGATG GAGGAAAGCC CTTTCAGTGT CCGGTATGCG GTCTGGTCAT CAAGAGAAAG 
AGCTACTGGA AGAGACATAT GGTGATCCAT ACAGGCTTAA AAAGCCACCA GTGTCCCCTC 
TGTCCCTTTC GCTGTGCTCG TAAAGACAAT CTCAAGTCCC ACATGAAGGT CCATCAGCAC 
CAAGACCGAG GGGAGACGTT CCAGTGTGAG CTCTGCCCCT TCACATCCTC CCGCCACTTT 
AGCCTCAAGC TGCACATGCG TTGCCATCAG CACTTCCCTC GTCCCGACCT CAAAGTCAAA 
GAAGAACCCG GCACAGACAC CGAGGAGGGC GAGGGCTTGC TCATGGGGGA CGGTGGAGCG 
GCTGGTGATC AGGCCATGAA CACAGATGCT TCTACGTCTC CCACTGTGAG CCAACCCGAC 
GCTCGTTTGC TGGCCTCTCC TGCAGAACCC TCAAACCACG TCCAGATCAA GGAGGAACCA 
CAGGAGAGGG ATGTGTCTGT GATGTCTCCC TTTGCCCTGT GTAGGGAGAG GCCGAGCAGC 
AGCAGCAGTT CTCTGGATCT GTCCGGGCTT AAATCTAGTC CACCGGGGCC CGGGCCTACA 
GCTGCTTCAC TCTTCAGCCC AGATATCACC ACCAAGACGG CTACAGACCT CCTCATAAAG 
CTCTCGGCAG CGAACCAGAA GGAGGCCCTA AAGGGTCCAT ACTGTGTCAA GGAAGAGCCC 
CAGGCGGAGG AGCCCCTGAA CTCTCAGAGG GGACCCAGCT ACAGCTTCTG CCAAGAAGGA 
CCTCCAGGAG CCACCAAACC AAGAGAGGAG GGGAGCCCGA TGTTGGGCAA GAGCAGCCTT 
CTCAGCCAGG ACATCAACGT CAAAGTGGCC TCTGAATTGC TTATGAAGCT CTCAGAGAAC 
AACAAAGACG CTTGCGTCCA GAATGTTATT GTGAAGGCGG AGCCTATGGA AGTGGATCCA 
CCCCTTGATC TGGCCTCACC CTCCTCCCAT CTACTGGGCT TTAGCACTTC AGGACCATGT 
GAAAAGAAGG AACCAATGGT CAACCTCCCA GATCATTTGC CCCGCCCCCA GAATGACCTC 
TTCTCGCAGG ACATATCGGT CAAAATGGCC TCCGAGCTGC TTTTCAAACT CTCTGAAAAA 
GTAAGTAAAG CTAATGAAAA CAAAGACAAT GCATCATTCG GTATACACAG CCCTTTCCTT 
GATGACCGCT TTAGACAATC ACCTTTCAGC ACTCGCTCCA AGAGCTCTTC CCCAGCTGAA 
GCCGGATCTT CAAGCAGGGT TCCCCAGCAA GATCCAGAGA AGGTGAGTAG TGAGCCAGGA 
AATGGACTTT CCCAATGGAG ACTCAACGAA CAGCTCTTTC CCTGCCCTGT GTGTGGCAAA 
GTGTTTGGTC GCCAACAGAC TCTCTCCAGG CATCTCTCCT TACACACAGA AGAGAGGAAA 
TACAAGTGTC ATTTGTGTCC ATATGCAGCT AAGTGCCGAG CCAACCTCAA CCAGCACCTC 
ACCATCCACT CAGTCAAACT GGTGAACACA GATGTCGAGC AGATTGTTAC CGCTGTTACC 
AACGATGGCC CAGAGCTCAA AAACTGCCCC TACTACTACA GCTGCCATGT CTGCGGCTTC 
CAGACAGAGT TCAATGCTCA GTTTGTCAGT CATATGTCTC TTCATGTGGA CAAAGAGCAG 
TGGATGTTTT CTCTCTGCTG TAGCACCTGT GAGTATGTGG GTGTGGACGA GGCCGAGATG 
AAAGCTCACA TCAGTGCAGG GCACGCAGGT CTCAATTCTA GAAGCCCTCT CAGTGAGACC 
AAGAGCACTT CCTCCTCTCT GTCAGCCCTC AGCGACTCCC TCAACAGCTC TGAAGGTGGA 
GATGTTGCCC AAGGTAACGA AGAACTAAAA AGCCTTTTGG CTCCGCCTTC ATCTGTGGGA 
AGCCAATCAA GCTTAGGCCC AGGAGCAGAG GAAAAAGCAG AGAAAGGCTT TGAATGCGTC 
TTCTGCAACT TTGTGTGTAA AACCCGACCC ATGTACGAGC GGCACCTACA GATCCACCTG 
ATCACGCGCA TGTTTGAGTG TGACGTCTGC CACAAGTTCA TGAAGACGCC CGAGCAGCTC 
CTGGAGCACA AGAAGTGCCA CACCGTCCCC GCTGGAGGGC TCAAGGTTTG TGAAGCGCTT 
TGGGGCCCTG AGCAGATGAA AGGTGCTATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf827 ( XM_005159977.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160034.1 Danio rerio zinc finger protein 827 (znf827), transcript variant X1, mRNA.

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CloneID	ODa05908
Clone ID Related Accession (Same CDS sequence)	XM_005159977.3 , XM_005159977.4
Accession Version	XM_005159977.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	zinc finger protein 827 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159977.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GGAAACAGGA GCAACCCAAG CGCCTGCCTT CACATGTGGA CACAGTAGAG 
GATGGAGGGC CAGAGAGTGG CTCAGTAGAG GAGGACAGCT GGTTTGGGAA TACCTCCGAC 
AGCCCCTCGG AATCGTCCTA CGGTGATGTG CAAGATGAGC TTCGGGTTTC TCCAGACAAC 
GTTCCCCGAC CGGACCACGA CACCTCAGCT GAGAGCTCCC TGGGCTGCCC GACGCCAGTT 
CAGCGGGCCT CTCTGGAGCT TCTGGGCCTG GACAGTGGGG TCCTTTCGAC CCAGGACACG 
CTTTCCTCTG TGGTGTCCTC TCTTTATGGA GACTCTAGCC AGGCTCTGGG TAAGCCACTC 
AGCAGTAATC TGCGCCGCCT CCTGGAGGCT GGCTCACTGA AGCTTGAGGG AGGGGAGCTG 
GCTGGACGTG GAACGGGTAG AGGACAAGAG TCACCCCCGG TCAGCCTGGT ACTATCTCCG 
TCTTCACATC ACGCACAGCA GCTGAGTGCC CTGGCGAGGA GGTTGGCAAA TAGCAGCAAC 
AACAGCGGAA ATTCAAACTC TGCGTCACCT GCATCTGTAG CAACTGTCAA GCAGGAGCCC 
TGTGACCCAT ACGGCCCCAG CAACGGCCCT AACTCAGGCA GCAGTGGCTT TGTATGGGTA 
GGCGTCGCAG ATAAATGGCC ATCGACAAGT TGCTCAGCAC CCAGTGGCAG TAGCCTCTCA 
CCAGACTCAG CAATTCAGAA GTTAAAGGCA GCCGCCAACG CCGTGCTCCA AGACAAGAAC 
GCGGTGGCCT CCTCCACCTC GGCATCTTCA TCCTCCTCGT CCTCCTCCTC ATCCTCTTCA 
GTCACCTCCT CCTCAATGGC AGCCCTGGGC ACTCGCTCTG ATGACGCAGT GCGCTTTGAA 
CCATTCGCTT CACCCTTTAG CCCGCAGTCA GCCAGCTCTA CGCTTGCTGC CCTCTCAAAG 
AAAGTGTCTG AAAGAAGTCA GCAGCAGGTG GGCTCATCTG AGCATCAACC CTCAGCTGGC 
TCCTTTCTCT CTCTGGTTTC CATGACCTCC TCAGCAGCTC TGCTAAAGGA GGTGGCCGCA 
CGAGCAGCAG GGAATCTTCT TGCTGATAAG AAAGAGCCGG CGTTAGTACT GGGAGCCTCC 
GAAGATGTCA AGCCCCTGTT AGACAGGAAC CAGAAGGTGC CCACCCCAAC CCAGGCAATG 
GATCTTCTGC TGCCAACAGT CCCCAAGGGA AGGGCCAAGC CCAGCAGTCA AGCAGGTTCT 
CCTGAGGATG GAGGAAAGCC CTTTCAGTGT CCGGTATGCG GTCTGGTCAT CAAGAGAAAG 
AGCTACTGGA AGAGACATAT GGTGATCCAT ACAGGCTTAA AAAGCCACCA GTGTCCCCTC 
TGTCCCTTTC GCTGTGCTCG TAAAGACAAT CTCAAGTCCC ACATGAAGGT CCATCAGCAC 
CAAGACCGAG GGGAGACGTT CCAGTGTGAG CTCTGCCCCT TCACATCCTC CCGCCACTTT 
AGCCTCAAGC TGCACATGCG TTGCCATCAG CACTTCCCTC GTCCCGACCT CAAAGTCAAA 
GAAGAACCCG GCACAGACAC CGAGGAGGGC GAGGGCTTGC TCATGGGGGA CGGTGGAGCG 
GCTGGTGATC AGGCCATGAA CACAGATGCT TCTACGTCTC CCACTGTGAG CCAACCCGAC 
GCTCGTTTGC TGGCCTCTCC TGCAGAACCC TCAAACCACG TCCAGATCAA GGAGGAACCA 
CAGGAGAGGG ATGTGTCTGT GATGTCTCCC TTTGCCCTGT GTAGGGAGAG GCCGAGCAGC 
AGCAGCAGTT CTCTGGATCT GTCCGGGCTT AAATCTAGTC CACCGGGGCC CGGGCCTACA 
GCTGCTTCAC TCTTCAGCCC AGATATCACC ACCAAGACGG CTACAGACCT CCTCATAAAG 
CTCTCGGCAG CGAACCAGAA GGAGGCCCTA AAGGGTCCAT ACTGTGTCAA GGAAGAGCCC 
CAGGCGGAGG AGCCCCTGAA CTCTCAGAGG GGACCCAGCT ACAGCTTCTG CCAAGAAGGA 
CCTCCAGGAG CCACCAAACC AAGAGAGGAG GGGAGCCCGA TGTTGGGCAA GAGCAGCCTT 
CTCAGCCAGG ACATCAACGT CAAAGTGGCC TCTGAATTGC TTATGAAGCT CTCAGAGAAC 
AACAAAGACG CTTGCGTCCA GAATGTTATT GTGAAGGCGG AGCCTATGGA AGTGGATCCA 
CCCCTTGATC TGGCCTCACC CTCCTCCCAT CTACTGGGCT TTAGCACTTC AGGACCATGT 
GAAAAGAAGG AACCAATGGT CAACCTCCCA GATCATTTGC CCCGCCCCCA GAATGACCTC 
TTCTCGCAGG ACATATCGGT CAAAATGGCC TCCGAGCTGC TTTTCAAACT CTCTGAAAAA 
GTAAGTAAAG CTAATGAAAA CAAAGACAAT GCATCATTCG GTATACACAG CCCTTTCCTT 
GATGACCGCT TTAGACAATC ACCTTTCAGC ACTCGCTCCA AGAGCTCTTC CCCAGCTGAA 
GCCGGATCTT CAAGCAGGGT TCCCCAGCAA GATCCAGAGA AGGTGAGTAG TGAGCCAGGA 
AATGGACTTT CCCAATGGAG ACTCAACGAA CAGCTCTTTC CCTGCCCTGT GTGTGGCAAA 
GTGTTTGGTC GCCAACAGAC TCTCTCCAGG CATCTCTCCT TACACACAGA AGAGAGGAAA 
TACAAGTGTC ATTTGTGTCC ATATGCAGCT AAGTGCCGAG CCAACCTCAA CCAGCACCTC 
ACCATCCACT CAGTCAAACT GGTGAACACA GATGTCGAGC AGATTGTTAC CGCTGTTACC 
AACGATGGCC CAGAGCTCAA AAACTGCCCC TACTACTACA GCTGCCATGT CTGCGGCTTC 
CAGACAGAGT TCAATGCTCA GTTTGTCAGT CATATGTCTC TTCATGTGGA CAAAGAGCAG 
TGGATGTTTT CTCTCTGCTG TAGCACCTGT GAGTATGTGG GTGTGGACGA GGCCGAGATG 
AAAGCTCACA TCAGTGCAGG GCACGCAGGT CTCAATTCTA GAAGCCCTCT CAGTGAGACC 
AAGAGCACTT CCTCCTCTCT GTCAGCCCTC AGCGACTCCC TCAACAGCTC TGAAGGTGGA 
GATGTTGCCC AAGGTAACGA AGAACTAAAA AGCCTTTTGG CTCCGCCTTC ATCTGTGGGA 
AGCCAATCAA GCTTAGGCCC AGGAGCAGAG GAAAAAGCAG AGAAAGGCTT TGAATGCGTC 
TTCTGCAACT TTGTGTGTAA AACCCGACCC ATGTACGAGC GGCACCTACA GATCCACCTG 
ATCACGCGCA TGTTTGAGTG TGACGTCTGC CACAAGTTCA TGAAGACGCC CGAGCAGCTC 
CTGGAGCACA AGAAGTGCCA CACCGTCCCC GCTGGAGGGC TCAAGTGCCC TCTCTGCATC 
TACTCCACCA ACAGGCCAGC CGCCATGGAG GGCCACCTGA AGACACACTA CAAGATGGAG 
TATCGCTGCC GCATCTGCCA AGCTGTGTGG GCCAATCAGG CAGAGCTGGA AAGGCACACG 
CGCGCTCACC GCCTGGGCAA CCACTATAAG TGTGAGCAGT GCGGCTACCT CTCCAAAACA 
GCCAACAAGC TCATCGAGCA TGTGCGCGTG CACACCGGCG AGCGGCCTTT CCACTGCGAC 
CGCTGCAGCT ACAGCTGCAA ACGCAAGGAC AATCTCAACC TCCACAAGCG GCTCAAGCAC 
GCACCGAGAC AGACCTTCGG TTGCGCCCAG TGCCCATTCA CCACCACTCA TCCGTTCGTC 
TACAGCCGCC ATGTCAAAAA GCACCAAGGC GGGGAGGGAA GCGCCGAGGA AGGCGAGGCT 
CGGCCAGGTA CGCCCCATCC GGGCCTTCTG GAGCGAGGTG GGAGCAGCAG CGGGAGCGGG 
GACAGTGGGA GCACCAGTCC GCTCATGAAC CTGTCGGCCT CTCAAGCCTT GCAGTCCGTC 
GCCCTGTCAA TCACCAGCAA GCAGCAAGAT GGCACGCCAA CCGCGCGCTA CCTCTCAGCC 
AATGGCACGT TGTCCACACT GGCGGCTCGC TACTTTGACC AGTACAGGAA CTGTGACCTG 
GCTCACCTCG TCCCCCTCAC CACGCTCTTT ACCACCCGAC GCTTCACATT CCACAGCCCC 
TCGCCCTCAA ACCCTTCGCC GCCCTTCTCT GCATTCTGGT CCAACTCATC CCCGCAGTCA 
CCACCCTCTC CCACATCACT CAAACACTCC TTCCTGGCAT ATCTGGGACT GACAGAGAGA 
GCGAAGACCA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160034.1
CDS	856..5130
Translation	MPRRKQEQPKRLPSHVDTVEDGGPESGSVEEDSWFGNTSDSPSESSYGDVQDELRVSPDNVPRPDHDTSAESSLGCPTPVQRASLELLGLDSGVLSTQDTLSSVVSSLYGDSSQALGKPLSSNLRRLLEAGSLKLEGGELAGRGTGRGQESPPVSLVLSPSSHHAQQLSALARRLANSSNNSGNSNSASPASVATVKQEPCDPYGPSNGPNSGSSGFVWVGVADKWPSTSCSAPSGSSLSPDSAIQKLKAAANAVLQDKNAVASSTSASSSSSSSSSSSSVTSSSMAALGTRSDDAVRFEPFASPFSPQSASSTLAALSKKVSERSQQQVGSSEHQPSAGSFLSLVSMTSSAALLKEVAARAAGNLLADKKEPALVLGASEDVKPLLDRNQKVPTPTQAMDLLLPTVPKGRAKPSSQAGSPEDGGKPFQCPVCGLVIKRKSYWKRHMVIHTGLKSHQCPLCPFRCARKDNLKSHMKVHQHQDRGETFQCELCPFTSSRHFSLKLHMRCHQHFPRPDLKVKEEPGTDTEEGEGLLMGDGGAAGDQAMNTDASTSPTVSQPDARLLASPAEPSNHVQIKEEPQERDVSVMSPFALCRERPSSSSSSLDLSGLKSSPPGPGPTAASLFSPDITTKTATDLLIKLSAANQKEALKGPYCVKEEPQAEEPLNSQRGPSYSFCQEGPPGATKPREEGSPMLGKSSLLSQDINVKVASELLMKLSENNKDACVQNVIVKAEPMEVDPPLDLASPSSHLLGFSTSGPCEKKEPMVNLPDHLPRPQNDLFSQDISVKMASELLFKLSEKVSKANENKDNASFGIHSPFLDDRFRQSPFSTRSKSSSPAEAGSSSRVPQQDPEKVSSEPGNGLSQWRLNEQLFPCPVCGKVFGRQQTLSRHLSLHTEERKYKCHLCPYAAKCRANLNQHLTIHSVKLVNTDVEQIVTAVTNDGPELKNCPYYYSCHVCGFQTEFNAQFVSHMSLHVDKEQWMFSLCCSTCEYVGVDEAEMKAHISAGHAGLNSRSPLSETKSTSSSLSALSDSLNSSEGGDVAQGNEELKSLLAPPSSVGSQSSLGPGAEEKAEKGFECVFCNFVCKTRPMYERHLQIHLITRMFECDVCHKFMKTPEQLLEHKKCHTVPAGGLKCPLCIYSTNRPAAMEGHLKTHYKMEYRCRICQAVWANQAELERHTRAHRLGNHYKCEQCGYLSKTANKLIEHVRVHTGERPFHCDRCSYSCKRKDNLNLHKRLKHAPRQTFGCAQCPFTTTHPFVYSRHVKKHQGGEGSAEEGEARPGTPHPGLLERGGSSSGSGDSGSTSPLMNLSASQALQSVALSITSKQQDGTPTARYLSANGTLSTLAARYFDQYRNCDLAHLVPLTTLFTTRRFTFHSPSPSNPSPPFSAFWSNSSPQSPPSPTSLKHSFLAYLGLTERAKTI

Target ORF information:

RefSeq Version	XM_005159977.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio zinc finger protein 827 (znf827), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159977.3

ORF Insert Sequence:

ATGCCAAGGA GGAAACAGGA GCAACCCAAG CGCCTGCCTT CACATGTGGA CACAGTAGAG 
GATGGAGGGC CAGAGAGTGG CTCAGTAGAG GAGGACAGCT GGTTTGGGAA TACCTCCGAC 
AGCCCCTCGG AATCGTCCTA CGGTGATGTG CAAGATGAGC TTCGGGTTTC TCCAGACAAC 
GTTCCCCGAC CGGACCACGA CACCTCAGCT GAGAGCTCCC TGGGCTGCCC GACGCCAGTT 
CAGCGGGCCT CTCTGGAGCT TCTGGGCCTG GACAGTGGGG TCCTTTCGAC CCAGGACACG 
CTTTCCTCTG TGGTGTCCTC TCTTTATGGA GACTCTAGCC AGGCTCTGGG TAAGCCACTC 
AGCAGTAATC TGCGCCGCCT CCTGGAGGCT GGCTCACTGA AGCTTGAGGG AGGGGAGCTG 
GCTGGACGTG GAACGGGTAG AGGACAAGAG TCACCCCCGG TCAGCCTGGT ACTATCTCCG 
TCTTCACATC ACGCACAGCA GCTGAGTGCC CTGGCGAGGA GGTTGGCAAA TAGCAGCAAC 
AACAGCGGAA ATTCAAACTC TGCGTCACCT GCATCTGTAG CAACTGTCAA GCAGGAGCCC 
TGTGACCCAT ACGGCCCCAG CAACGGCCCT AACTCAGGCA GCAGTGGCTT TGTATGGGTA 
GGCGTCGCAG ATAAATGGCC ATCGACAAGT TGCTCAGCAC CCAGTGGCAG TAGCCTCTCA 
CCAGACTCAG CAATTCAGAA GTTAAAGGCA GCCGCCAACG CCGTGCTCCA AGACAAGAAC 
GCGGTGGCCT CCTCCACCTC GGCATCTTCA TCCTCCTCGT CCTCCTCCTC ATCCTCTTCA 
GTCACCTCCT CCTCAATGGC AGCCCTGGGC ACTCGCTCTG ATGACGCAGT GCGCTTTGAA 
CCATTCGCTT CACCCTTTAG CCCGCAGTCA GCCAGCTCTA CGCTTGCTGC CCTCTCAAAG 
AAAGTGTCTG AAAGAAGTCA GCAGCAGGTG GGCTCATCTG AGCATCAACC CTCAGCTGGC 
TCCTTTCTCT CTCTGGTTTC CATGACCTCC TCAGCAGCTC TGCTAAAGGA GGTGGCCGCA 
CGAGCAGCAG GGAATCTTCT TGCTGATAAG AAAGAGCCGG CGTTAGTACT GGGAGCCTCC 
GAAGATGTCA AGCCCCTGTT AGACAGGAAC CAGAAGGTGC CCACCCCAAC CCAGGCAATG 
GATCTTCTGC TGCCAACAGT CCCCAAGGGA AGGGCCAAGC CCAGCAGTCA AGCAGGTTCT 
CCTGAGGATG GAGGAAAGCC CTTTCAGTGT CCGGTATGCG GTCTGGTCAT CAAGAGAAAG 
AGCTACTGGA AGAGACATAT GGTGATCCAT ACAGGCTTAA AAAGCCACCA GTGTCCCCTC 
TGTCCCTTTC GCTGTGCTCG TAAAGACAAT CTCAAGTCCC ACATGAAGGT CCATCAGCAC 
CAAGACCGAG GGGAGACGTT CCAGTGTGAG CTCTGCCCCT TCACATCCTC CCGCCACTTT 
AGCCTCAAGC TGCACATGCG TTGCCATCAG CACTTCCCTC GTCCCGACCT CAAAGTCAAA 
GAAGAACCCG GCACAGACAC CGAGGAGGGC GAGGGCTTGC TCATGGGGGA CGGTGGAGCG 
GCTGGTGATC AGGCCATGAA CACAGATGCT TCTACGTCTC CCACTGTGAG CCAACCCGAC 
GCTCGTTTGC TGGCCTCTCC TGCAGAACCC TCAAACCACG TCCAGATCAA GGAGGAACCA 
CAGGAGAGGG ATGTGTCTGT GATGTCTCCC TTTGCCCTGT GTAGGGAGAG GCCGAGCAGC 
AGCAGCAGTT CTCTGGATCT GTCCGGGCTT AAATCTAGTC CACCGGGGCC CGGGCCTACA 
GCTGCTTCAC TCTTCAGCCC AGATATCACC ACCAAGACGG CTACAGACCT CCTCATAAAG 
CTCTCGGCAG CGAACCAGAA GGAGGCCCTA AAGGGTCCAT ACTGTGTCAA GGAAGAGCCC 
CAGGCGGAGG AGCCCCTGAA CTCTCAGAGG GGACCCAGCT ACAGCTTCTG CCAAGAAGGA 
CCTCCAGGAG CCACCAAACC AAGAGAGGAG GGGAGCCCGA TGTTGGGCAA GAGCAGCCTT 
CTCAGCCAGG ACATCAACGT CAAAGTGGCC TCTGAATTGC TTATGAAGCT CTCAGAGAAC 
AACAAAGACG CTTGCGTCCA GAATGTTATT GTGAAGGCGG AGCCTATGGA AGTGGATCCA 
CCCCTTGATC TGGCCTCACC CTCCTCCCAT CTACTGGGCT TTAGCACTTC AGGACCATGT 
GAAAAGAAGG AACCAATGGT CAACCTCCCA GATCATTTGC CCCGCCCCCA GAATGACCTC 
TTCTCGCAGG ACATATCGGT CAAAATGGCC TCCGAGCTGC TTTTCAAACT CTCTGAAAAA 
GTAAGTAAAG CTAATGAAAA CAAAGACAAT GCATCATTCG GTATACACAG CCCTTTCCTT 
GATGACCGCT TTAGACAATC ACCTTTCAGC ACTCGCTCCA AGAGCTCTTC CCCAGCTGAA 
GCCGGATCTT CAAGCAGGGT TCCCCAGCAA GATCCAGAGA AGGTGAGTAG TGAGCCAGGA 
AATGGACTTT CCCAATGGAG ACTCAACGAA CAGCTCTTTC CCTGCCCTGT GTGTGGCAAA 
GTGTTTGGTC GCCAACAGAC TCTCTCCAGG CATCTCTCCT TACACACAGA AGAGAGGAAA 
TACAAGTGTC ATTTGTGTCC ATATGCAGCT AAGTGCCGAG CCAACCTCAA CCAGCACCTC 
ACCATCCACT CAGTCAAACT GGTGAACACA GATGTCGAGC AGATTGTTAC CGCTGTTACC 
AACGATGGCC CAGAGCTCAA AAACTGCCCC TACTACTACA GCTGCCATGT CTGCGGCTTC 
CAGACAGAGT TCAATGCTCA GTTTGTCAGT CATATGTCTC TTCATGTGGA CAAAGAGCAG 
TGGATGTTTT CTCTCTGCTG TAGCACCTGT GAGTATGTGG GTGTGGACGA GGCCGAGATG 
AAAGCTCACA TCAGTGCAGG GCACGCAGGT CTCAATTCTA GAAGCCCTCT CAGTGAGACC 
AAGAGCACTT CCTCCTCTCT GTCAGCCCTC AGCGACTCCC TCAACAGCTC TGAAGGTGGA 
GATGTTGCCC AAGGTAACGA AGAACTAAAA AGCCTTTTGG CTCCGCCTTC ATCTGTGGGA 
AGCCAATCAA GCTTAGGCCC AGGAGCAGAG GAAAAAGCAG AGAAAGGCTT TGAATGCGTC 
TTCTGCAACT TTGTGTGTAA AACCCGACCC ATGTACGAGC GGCACCTACA GATCCACCTG 
ATCACGCGCA TGTTTGAGTG TGACGTCTGC CACAAGTTCA TGAAGACGCC CGAGCAGCTC 
CTGGAGCACA AGAAGTGCCA CACCGTCCCC GCTGGAGGGC TCAAGTGCCC TCTCTGCATC 
TACTCCACCA ACAGGCCAGC CGCCATGGAG GGCCACCTGA AGACACACTA CAAGATGGAG 
TATCGCTGCC GCATCTGCCA AGCTGTGTGG GCCAATCAGG CAGAGCTGGA AAGGCACACG 
CGCGCTCACC GCCTGGGCAA CCACTATAAG TGTGAGCAGT GCGGCTACCT CTCCAAAACA 
GCCAACAAGC TCATCGAGCA TGTGCGCGTG CACACCGGCG AGCGGCCTTT CCACTGCGAC 
CGCTGCAGCT ACAGCTGCAA ACGCAAGGAC AATCTCAACC TCCACAAGCG GCTCAAGCAC 
GCACCGAGAC AGACCTTCGG TTGCGCCCAG TGCCCATTCA CCACCACTCA TCCGTTCGTC 
TACAGCCGCC ATGTCAAAAA GCACCAAGGC GGGGAGGGAA GCGCCGAGGA AGGCGAGGCT 
CGGCCAGGTA CGCCCCATCC GGGCCTTCTG GAGCGAGGTG GGAGCAGCAG CGGGAGCGGG 
GACAGTGGGA GCACCAGTCC GCTCATGAAC CTGTCGGCCT CTCAAGCCTT GCAGTCCGTC 
GCCCTGTCAA TCACCAGCAA GCAGCAAGAT GGCACGCCAA CCGCGCGCTA CCTCTCAGCC 
AATGGCACGT TGTCCACACT GGCGGCTCGC TACTTTGACC AGTACAGGAA CTGTGACCTG 
GCTCACCTCG TCCCCCTCAC CACGCTCTTT ACCACCCGAC GCTTCACATT CCACAGCCCC 
TCGCCCTCAA ACCCTTCGCC GCCCTTCTCT GCATTCTGGT CCAACTCATC CCCGCAGTCA 
CCACCCTCTC CCACATCACT CAAACACTCC TTCCTGGCAT ATCTGGGACT GACAGAGAGA 
GCGAAGACCA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf827 ( XM_005159977.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005160034.1 Danio rerio zinc finger protein 827 (znf827), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa05908
Clone ID Related Accession (Same CDS sequence)	XM_005159977.3 , XM_005159977.4
Accession Version	XM_005159977.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	zinc finger protein 827 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159977.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GGAAACAGGA GCAACCCAAG CGCCTGCCTT CACATGTGGA CACAGTAGAG 
GATGGAGGGC CAGAGAGTGG CTCAGTAGAG GAGGACAGCT GGTTTGGGAA TACCTCCGAC 
AGCCCCTCGG AATCGTCCTA CGGTGATGTG CAAGATGAGC TTCGGGTTTC TCCAGACAAC 
GTTCCCCGAC CGGACCACGA CACCTCAGCT GAGAGCTCCC TGGGCTGCCC GACGCCAGTT 
CAGCGGGCCT CTCTGGAGCT TCTGGGCCTG GACAGTGGGG TCCTTTCGAC CCAGGACACG 
CTTTCCTCTG TGGTGTCCTC TCTTTATGGA GACTCTAGCC AGGCTCTGGG TAAGCCACTC 
AGCAGTAATC TGCGCCGCCT CCTGGAGGCT GGCTCACTGA AGCTTGAGGG AGGGGAGCTG 
GCTGGACGTG GAACGGGTAG AGGACAAGAG TCACCCCCGG TCAGCCTGGT ACTATCTCCG 
TCTTCACATC ACGCACAGCA GCTGAGTGCC CTGGCGAGGA GGTTGGCAAA TAGCAGCAAC 
AACAGCGGAA ATTCAAACTC TGCGTCACCT GCATCTGTAG CAACTGTCAA GCAGGAGCCC 
TGTGACCCAT ACGGCCCCAG CAACGGCCCT AACTCAGGCA GCAGTGGCTT TGTATGGGTA 
GGCGTCGCAG ATAAATGGCC ATCGACAAGT TGCTCAGCAC CCAGTGGCAG TAGCCTCTCA 
CCAGACTCAG CAATTCAGAA GTTAAAGGCA GCCGCCAACG CCGTGCTCCA AGACAAGAAC 
GCGGTGGCCT CCTCCACCTC GGCATCTTCA TCCTCCTCGT CCTCCTCCTC ATCCTCTTCA 
GTCACCTCCT CCTCAATGGC AGCCCTGGGC ACTCGCTCTG ATGACGCAGT GCGCTTTGAA 
CCATTCGCTT CACCCTTTAG CCCGCAGTCA GCCAGCTCTA CGCTTGCTGC CCTCTCAAAG 
AAAGTGTCTG AAAGAAGTCA GCAGCAGGTG GGCTCATCTG AGCATCAACC CTCAGCTGGC 
TCCTTTCTCT CTCTGGTTTC CATGACCTCC TCAGCAGCTC TGCTAAAGGA GGTGGCCGCA 
CGAGCAGCAG GGAATCTTCT TGCTGATAAG AAAGAGCCGG CGTTAGTACT GGGAGCCTCC 
GAAGATGTCA AGCCCCTGTT AGACAGGAAC CAGAAGGTGC CCACCCCAAC CCAGGCAATG 
GATCTTCTGC TGCCAACAGT CCCCAAGGGA AGGGCCAAGC CCAGCAGTCA AGCAGGTTCT 
CCTGAGGATG GAGGAAAGCC CTTTCAGTGT CCGGTATGCG GTCTGGTCAT CAAGAGAAAG 
AGCTACTGGA AGAGACATAT GGTGATCCAT ACAGGCTTAA AAAGCCACCA GTGTCCCCTC 
TGTCCCTTTC GCTGTGCTCG TAAAGACAAT CTCAAGTCCC ACATGAAGGT CCATCAGCAC 
CAAGACCGAG GGGAGACGTT CCAGTGTGAG CTCTGCCCCT TCACATCCTC CCGCCACTTT 
AGCCTCAAGC TGCACATGCG TTGCCATCAG CACTTCCCTC GTCCCGACCT CAAAGTCAAA 
GAAGAACCCG GCACAGACAC CGAGGAGGGC GAGGGCTTGC TCATGGGGGA CGGTGGAGCG 
GCTGGTGATC AGGCCATGAA CACAGATGCT TCTACGTCTC CCACTGTGAG CCAACCCGAC 
GCTCGTTTGC TGGCCTCTCC TGCAGAACCC TCAAACCACG TCCAGATCAA GGAGGAACCA 
CAGGAGAGGG ATGTGTCTGT GATGTCTCCC TTTGCCCTGT GTAGGGAGAG GCCGAGCAGC 
AGCAGCAGTT CTCTGGATCT GTCCGGGCTT AAATCTAGTC CACCGGGGCC CGGGCCTACA 
GCTGCTTCAC TCTTCAGCCC AGATATCACC ACCAAGACGG CTACAGACCT CCTCATAAAG 
CTCTCGGCAG CGAACCAGAA GGAGGCCCTA AAGGGTCCAT ACTGTGTCAA GGAAGAGCCC 
CAGGCGGAGG AGCCCCTGAA CTCTCAGAGG GGACCCAGCT ACAGCTTCTG CCAAGAAGGA 
CCTCCAGGAG CCACCAAACC AAGAGAGGAG GGGAGCCCGA TGTTGGGCAA GAGCAGCCTT 
CTCAGCCAGG ACATCAACGT CAAAGTGGCC TCTGAATTGC TTATGAAGCT CTCAGAGAAC 
AACAAAGACG CTTGCGTCCA GAATGTTATT GTGAAGGCGG AGCCTATGGA AGTGGATCCA 
CCCCTTGATC TGGCCTCACC CTCCTCCCAT CTACTGGGCT TTAGCACTTC AGGACCATGT 
GAAAAGAAGG AACCAATGGT CAACCTCCCA GATCATTTGC CCCGCCCCCA GAATGACCTC 
TTCTCGCAGG ACATATCGGT CAAAATGGCC TCCGAGCTGC TTTTCAAACT CTCTGAAAAA 
GTAAGTAAAG CTAATGAAAA CAAAGACAAT GCATCATTCG GTATACACAG CCCTTTCCTT 
GATGACCGCT TTAGACAATC ACCTTTCAGC ACTCGCTCCA AGAGCTCTTC CCCAGCTGAA 
GCCGGATCTT CAAGCAGGGT TCCCCAGCAA GATCCAGAGA AGGTGAGTAG TGAGCCAGGA 
AATGGACTTT CCCAATGGAG ACTCAACGAA CAGCTCTTTC CCTGCCCTGT GTGTGGCAAA 
GTGTTTGGTC GCCAACAGAC TCTCTCCAGG CATCTCTCCT TACACACAGA AGAGAGGAAA 
TACAAGTGTC ATTTGTGTCC ATATGCAGCT AAGTGCCGAG CCAACCTCAA CCAGCACCTC 
ACCATCCACT CAGTCAAACT GGTGAACACA GATGTCGAGC AGATTGTTAC CGCTGTTACC 
AACGATGGCC CAGAGCTCAA AAACTGCCCC TACTACTACA GCTGCCATGT CTGCGGCTTC 
CAGACAGAGT TCAATGCTCA GTTTGTCAGT CATATGTCTC TTCATGTGGA CAAAGAGCAG 
TGGATGTTTT CTCTCTGCTG TAGCACCTGT GAGTATGTGG GTGTGGACGA GGCCGAGATG 
AAAGCTCACA TCAGTGCAGG GCACGCAGGT CTCAATTCTA GAAGCCCTCT CAGTGAGACC 
AAGAGCACTT CCTCCTCTCT GTCAGCCCTC AGCGACTCCC TCAACAGCTC TGAAGGTGGA 
GATGTTGCCC AAGGTAACGA AGAACTAAAA AGCCTTTTGG CTCCGCCTTC ATCTGTGGGA 
AGCCAATCAA GCTTAGGCCC AGGAGCAGAG GAAAAAGCAG AGAAAGGCTT TGAATGCGTC 
TTCTGCAACT TTGTGTGTAA AACCCGACCC ATGTACGAGC GGCACCTACA GATCCACCTG 
ATCACGCGCA TGTTTGAGTG TGACGTCTGC CACAAGTTCA TGAAGACGCC CGAGCAGCTC 
CTGGAGCACA AGAAGTGCCA CACCGTCCCC GCTGGAGGGC TCAAGTGCCC TCTCTGCATC 
TACTCCACCA ACAGGCCAGC CGCCATGGAG GGCCACCTGA AGACACACTA CAAGATGGAG 
TATCGCTGCC GCATCTGCCA AGCTGTGTGG GCCAATCAGG CAGAGCTGGA AAGGCACACG 
CGCGCTCACC GCCTGGGCAA CCACTATAAG TGTGAGCAGT GCGGCTACCT CTCCAAAACA 
GCCAACAAGC TCATCGAGCA TGTGCGCGTG CACACCGGCG AGCGGCCTTT CCACTGCGAC 
CGCTGCAGCT ACAGCTGCAA ACGCAAGGAC AATCTCAACC TCCACAAGCG GCTCAAGCAC 
GCACCGAGAC AGACCTTCGG TTGCGCCCAG TGCCCATTCA CCACCACTCA TCCGTTCGTC 
TACAGCCGCC ATGTCAAAAA GCACCAAGGC GGGGAGGGAA GCGCCGAGGA AGGCGAGGCT 
CGGCCAGGTA CGCCCCATCC GGGCCTTCTG GAGCGAGGTG GGAGCAGCAG CGGGAGCGGG 
GACAGTGGGA GCACCAGTCC GCTCATGAAC CTGTCGGCCT CTCAAGCCTT GCAGTCCGTC 
GCCCTGTCAA TCACCAGCAA GCAGCAAGAT GGCACGCCAA CCGCGCGCTA CCTCTCAGCC 
AATGGCACGT TGTCCACACT GGCGGCTCGC TACTTTGACC AGTACAGGAA CTGTGACCTG 
GCTCACCTCG TCCCCCTCAC CACGCTCTTT ACCACCCGAC GCTTCACATT CCACAGCCCC 
TCGCCCTCAA ACCCTTCGCC GCCCTTCTCT GCATTCTGGT CCAACTCATC CCCGCAGTCA 
CCACCCTCTC CCACATCACT CAAACACTCC TTCCTGGCAT ATCTGGGACT GACAGAGAGA 
GCGAAGACCA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005160034.1
CDS	412..4686
Translation	MPRRKQEQPKRLPSHVDTVEDGGPESGSVEEDSWFGNTSDSPSESSYGDVQDELRVSPDNVPRPDHDTSAESSLGCPTPVQRASLELLGLDSGVLSTQDTLSSVVSSLYGDSSQALGKPLSSNLRRLLEAGSLKLEGGELAGRGTGRGQESPPVSLVLSPSSHHAQQLSALARRLANSSNNSGNSNSASPASVATVKQEPCDPYGPSNGPNSGSSGFVWVGVADKWPSTSCSAPSGSSLSPDSAIQKLKAAANAVLQDKNAVASSTSASSSSSSSSSSSSVTSSSMAALGTRSDDAVRFEPFASPFSPQSASSTLAALSKKVSERSQQQVGSSEHQPSAGSFLSLVSMTSSAALLKEVAARAAGNLLADKKEPALVLGASEDVKPLLDRNQKVPTPTQAMDLLLPTVPKGRAKPSSQAGSPEDGGKPFQCPVCGLVIKRKSYWKRHMVIHTGLKSHQCPLCPFRCARKDNLKSHMKVHQHQDRGETFQCELCPFTSSRHFSLKLHMRCHQHFPRPDLKVKEEPGTDTEEGEGLLMGDGGAAGDQAMNTDASTSPTVSQPDARLLASPAEPSNHVQIKEEPQERDVSVMSPFALCRERPSSSSSSLDLSGLKSSPPGPGPTAASLFSPDITTKTATDLLIKLSAANQKEALKGPYCVKEEPQAEEPLNSQRGPSYSFCQEGPPGATKPREEGSPMLGKSSLLSQDINVKVASELLMKLSENNKDACVQNVIVKAEPMEVDPPLDLASPSSHLLGFSTSGPCEKKEPMVNLPDHLPRPQNDLFSQDISVKMASELLFKLSEKVSKANENKDNASFGIHSPFLDDRFRQSPFSTRSKSSSPAEAGSSSRVPQQDPEKVSSEPGNGLSQWRLNEQLFPCPVCGKVFGRQQTLSRHLSLHTEERKYKCHLCPYAAKCRANLNQHLTIHSVKLVNTDVEQIVTAVTNDGPELKNCPYYYSCHVCGFQTEFNAQFVSHMSLHVDKEQWMFSLCCSTCEYVGVDEAEMKAHISAGHAGLNSRSPLSETKSTSSSLSALSDSLNSSEGGDVAQGNEELKSLLAPPSSVGSQSSLGPGAEEKAEKGFECVFCNFVCKTRPMYERHLQIHLITRMFECDVCHKFMKTPEQLLEHKKCHTVPAGGLKCPLCIYSTNRPAAMEGHLKTHYKMEYRCRICQAVWANQAELERHTRAHRLGNHYKCEQCGYLSKTANKLIEHVRVHTGERPFHCDRCSYSCKRKDNLNLHKRLKHAPRQTFGCAQCPFTTTHPFVYSRHVKKHQGGEGSAEEGEARPGTPHPGLLERGGSSSGSGDSGSTSPLMNLSASQALQSVALSITSKQQDGTPTARYLSANGTLSTLAARYFDQYRNCDLAHLVPLTTLFTTRRFTFHSPSPSNPSPPFSAFWSNSSPQSPPSPTSLKHSFLAYLGLTERAKTI

Target ORF information:

RefSeq Version	XM_005159977.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zinc finger protein 827 (znf827), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159977.4

ORF Insert Sequence:

ATGCCAAGGA GGAAACAGGA GCAACCCAAG CGCCTGCCTT CACATGTGGA CACAGTAGAG 
GATGGAGGGC CAGAGAGTGG CTCAGTAGAG GAGGACAGCT GGTTTGGGAA TACCTCCGAC 
AGCCCCTCGG AATCGTCCTA CGGTGATGTG CAAGATGAGC TTCGGGTTTC TCCAGACAAC 
GTTCCCCGAC CGGACCACGA CACCTCAGCT GAGAGCTCCC TGGGCTGCCC GACGCCAGTT 
CAGCGGGCCT CTCTGGAGCT TCTGGGCCTG GACAGTGGGG TCCTTTCGAC CCAGGACACG 
CTTTCCTCTG TGGTGTCCTC TCTTTATGGA GACTCTAGCC AGGCTCTGGG TAAGCCACTC 
AGCAGTAATC TGCGCCGCCT CCTGGAGGCT GGCTCACTGA AGCTTGAGGG AGGGGAGCTG 
GCTGGACGTG GAACGGGTAG AGGACAAGAG TCACCCCCGG TCAGCCTGGT ACTATCTCCG 
TCTTCACATC ACGCACAGCA GCTGAGTGCC CTGGCGAGGA GGTTGGCAAA TAGCAGCAAC 
AACAGCGGAA ATTCAAACTC TGCGTCACCT GCATCTGTAG CAACTGTCAA GCAGGAGCCC 
TGTGACCCAT ACGGCCCCAG CAACGGCCCT AACTCAGGCA GCAGTGGCTT TGTATGGGTA 
GGCGTCGCAG ATAAATGGCC ATCGACAAGT TGCTCAGCAC CCAGTGGCAG TAGCCTCTCA 
CCAGACTCAG CAATTCAGAA GTTAAAGGCA GCCGCCAACG CCGTGCTCCA AGACAAGAAC 
GCGGTGGCCT CCTCCACCTC GGCATCTTCA TCCTCCTCGT CCTCCTCCTC ATCCTCTTCA 
GTCACCTCCT CCTCAATGGC AGCCCTGGGC ACTCGCTCTG ATGACGCAGT GCGCTTTGAA 
CCATTCGCTT CACCCTTTAG CCCGCAGTCA GCCAGCTCTA CGCTTGCTGC CCTCTCAAAG 
AAAGTGTCTG AAAGAAGTCA GCAGCAGGTG GGCTCATCTG AGCATCAACC CTCAGCTGGC 
TCCTTTCTCT CTCTGGTTTC CATGACCTCC TCAGCAGCTC TGCTAAAGGA GGTGGCCGCA 
CGAGCAGCAG GGAATCTTCT TGCTGATAAG AAAGAGCCGG CGTTAGTACT GGGAGCCTCC 
GAAGATGTCA AGCCCCTGTT AGACAGGAAC CAGAAGGTGC CCACCCCAAC CCAGGCAATG 
GATCTTCTGC TGCCAACAGT CCCCAAGGGA AGGGCCAAGC CCAGCAGTCA AGCAGGTTCT 
CCTGAGGATG GAGGAAAGCC CTTTCAGTGT CCGGTATGCG GTCTGGTCAT CAAGAGAAAG 
AGCTACTGGA AGAGACATAT GGTGATCCAT ACAGGCTTAA AAAGCCACCA GTGTCCCCTC 
TGTCCCTTTC GCTGTGCTCG TAAAGACAAT CTCAAGTCCC ACATGAAGGT CCATCAGCAC 
CAAGACCGAG GGGAGACGTT CCAGTGTGAG CTCTGCCCCT TCACATCCTC CCGCCACTTT 
AGCCTCAAGC TGCACATGCG TTGCCATCAG CACTTCCCTC GTCCCGACCT CAAAGTCAAA 
GAAGAACCCG GCACAGACAC CGAGGAGGGC GAGGGCTTGC TCATGGGGGA CGGTGGAGCG 
GCTGGTGATC AGGCCATGAA CACAGATGCT TCTACGTCTC CCACTGTGAG CCAACCCGAC 
GCTCGTTTGC TGGCCTCTCC TGCAGAACCC TCAAACCACG TCCAGATCAA GGAGGAACCA 
CAGGAGAGGG ATGTGTCTGT GATGTCTCCC TTTGCCCTGT GTAGGGAGAG GCCGAGCAGC 
AGCAGCAGTT CTCTGGATCT GTCCGGGCTT AAATCTAGTC CACCGGGGCC CGGGCCTACA 
GCTGCTTCAC TCTTCAGCCC AGATATCACC ACCAAGACGG CTACAGACCT CCTCATAAAG 
CTCTCGGCAG CGAACCAGAA GGAGGCCCTA AAGGGTCCAT ACTGTGTCAA GGAAGAGCCC 
CAGGCGGAGG AGCCCCTGAA CTCTCAGAGG GGACCCAGCT ACAGCTTCTG CCAAGAAGGA 
CCTCCAGGAG CCACCAAACC AAGAGAGGAG GGGAGCCCGA TGTTGGGCAA GAGCAGCCTT 
CTCAGCCAGG ACATCAACGT CAAAGTGGCC TCTGAATTGC TTATGAAGCT CTCAGAGAAC 
AACAAAGACG CTTGCGTCCA GAATGTTATT GTGAAGGCGG AGCCTATGGA AGTGGATCCA 
CCCCTTGATC TGGCCTCACC CTCCTCCCAT CTACTGGGCT TTAGCACTTC AGGACCATGT 
GAAAAGAAGG AACCAATGGT CAACCTCCCA GATCATTTGC CCCGCCCCCA GAATGACCTC 
TTCTCGCAGG ACATATCGGT CAAAATGGCC TCCGAGCTGC TTTTCAAACT CTCTGAAAAA 
GTAAGTAAAG CTAATGAAAA CAAAGACAAT GCATCATTCG GTATACACAG CCCTTTCCTT 
GATGACCGCT TTAGACAATC ACCTTTCAGC ACTCGCTCCA AGAGCTCTTC CCCAGCTGAA 
GCCGGATCTT CAAGCAGGGT TCCCCAGCAA GATCCAGAGA AGGTGAGTAG TGAGCCAGGA 
AATGGACTTT CCCAATGGAG ACTCAACGAA CAGCTCTTTC CCTGCCCTGT GTGTGGCAAA 
GTGTTTGGTC GCCAACAGAC TCTCTCCAGG CATCTCTCCT TACACACAGA AGAGAGGAAA 
TACAAGTGTC ATTTGTGTCC ATATGCAGCT AAGTGCCGAG CCAACCTCAA CCAGCACCTC 
ACCATCCACT CAGTCAAACT GGTGAACACA GATGTCGAGC AGATTGTTAC CGCTGTTACC 
AACGATGGCC CAGAGCTCAA AAACTGCCCC TACTACTACA GCTGCCATGT CTGCGGCTTC 
CAGACAGAGT TCAATGCTCA GTTTGTCAGT CATATGTCTC TTCATGTGGA CAAAGAGCAG 
TGGATGTTTT CTCTCTGCTG TAGCACCTGT GAGTATGTGG GTGTGGACGA GGCCGAGATG 
AAAGCTCACA TCAGTGCAGG GCACGCAGGT CTCAATTCTA GAAGCCCTCT CAGTGAGACC 
AAGAGCACTT CCTCCTCTCT GTCAGCCCTC AGCGACTCCC TCAACAGCTC TGAAGGTGGA 
GATGTTGCCC AAGGTAACGA AGAACTAAAA AGCCTTTTGG CTCCGCCTTC ATCTGTGGGA 
AGCCAATCAA GCTTAGGCCC AGGAGCAGAG GAAAAAGCAG AGAAAGGCTT TGAATGCGTC 
TTCTGCAACT TTGTGTGTAA AACCCGACCC ATGTACGAGC GGCACCTACA GATCCACCTG 
ATCACGCGCA TGTTTGAGTG TGACGTCTGC CACAAGTTCA TGAAGACGCC CGAGCAGCTC 
CTGGAGCACA AGAAGTGCCA CACCGTCCCC GCTGGAGGGC TCAAGTGCCC TCTCTGCATC 
TACTCCACCA ACAGGCCAGC CGCCATGGAG GGCCACCTGA AGACACACTA CAAGATGGAG 
TATCGCTGCC GCATCTGCCA AGCTGTGTGG GCCAATCAGG CAGAGCTGGA AAGGCACACG 
CGCGCTCACC GCCTGGGCAA CCACTATAAG TGTGAGCAGT GCGGCTACCT CTCCAAAACA 
GCCAACAAGC TCATCGAGCA TGTGCGCGTG CACACCGGCG AGCGGCCTTT CCACTGCGAC 
CGCTGCAGCT ACAGCTGCAA ACGCAAGGAC AATCTCAACC TCCACAAGCG GCTCAAGCAC 
GCACCGAGAC AGACCTTCGG TTGCGCCCAG TGCCCATTCA CCACCACTCA TCCGTTCGTC 
TACAGCCGCC ATGTCAAAAA GCACCAAGGC GGGGAGGGAA GCGCCGAGGA AGGCGAGGCT 
CGGCCAGGTA CGCCCCATCC GGGCCTTCTG GAGCGAGGTG GGAGCAGCAG CGGGAGCGGG 
GACAGTGGGA GCACCAGTCC GCTCATGAAC CTGTCGGCCT CTCAAGCCTT GCAGTCCGTC 
GCCCTGTCAA TCACCAGCAA GCAGCAAGAT GGCACGCCAA CCGCGCGCTA CCTCTCAGCC 
AATGGCACGT TGTCCACACT GGCGGCTCGC TACTTTGACC AGTACAGGAA CTGTGACCTG 
GCTCACCTCG TCCCCCTCAC CACGCTCTTT ACCACCCGAC GCTTCACATT CCACAGCCCC 
TCGCCCTCAA ACCCTTCGCC GCCCTTCTCT GCATTCTGGT CCAACTCATC CCCGCAGTCA 
CCACCCTCTC CCACATCACT CAAACACTCC TTCCTGGCAT ATCTGGGACT GACAGAGAGA 
GCGAAGACCA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf827 ( XM_001919166.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001919201.4 Danio rerio zinc finger protein 827 (znf827), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa05909
Clone ID Related Accession (Same CDS sequence)	XM_001919166.7 , XM_001919166.6
Accession Version	XM_001919166.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	zinc finger protein 827 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001919166.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GGAAACAGGA GCAACCCAAG CGCCTGCCTT CACATGTGGA CACAGTAGAG 
GATGGAGGGC CAGAGAGTGG CTCAGTAGAG GAGGACAGCT GGTTTGGGAA TACCTCCGAC 
AGCCCCTCGG AATCGTCCTA CGGTGATGTG CAAGATGAGC TTCGGGTTTC TCCAGACAAC 
GTTCCCCGAC CGGACCACGA CACCTCAGCT GAGAGCTCCC TGGGCTGCCC GACGCCAGTT 
CAGCGGGCCT CTCTGGAGCT TCTGGGCCTG GACAGTGGGG TCCTTTCGAC CCAGGACACG 
CTTTCCTCTG TGGTGTCCTC TCTTTATGGA GACTCTAGCC AGGCTCTGGG TAAGCCACTC 
AGCAGTAATC TGCGCCGCCT CCTGGAGGCT GGCTCACTGA AGCTTGAGGG AGGGGAGCTG 
GCTGGACGTG GAACGGGTAG AGGACAAGAG TCACCCCCGG TCAGCCTGGT ACTATCTCCG 
TCTTCACATC ACGCACAGCA GCTGAGTGCC CTGGCGAGGA GGTTGGCAAA TAGCAGCAAC 
AACAGCGGAA ATTCAAACTC TGCGTCACCT GCATCTGTAG CAACTGTCAA GCAGGAGCCC 
TGTGACCCAT ACGGCCCCAG CAACGGCCCT AACTCAGGCA GCAGTGGCTT TGTATGGGTA 
GGCGTCGCAG ATAAATGGCC ATCGACAAGT TGCTCAGCAC CCAGTGGCAG TAGCCTCTCA 
CCAGACTCAG CAATTCAGAA GTTAAAGGCA GCCGCCAACG CCGTGCTCCA AGACAAGAAC 
GCGGTGGCCT CCTCCACCTC GGCATCTTCA TCCTCCTCGT CCTCCTCCTC ATCCTCTTCA 
GTCACCTCCT CCTCAATGGC AGCCCTGGGC ACTCGCTCTG ATGACGCAGT GCGCTTTGAA 
CCATTCGCTT CACCCTTTAG CCCGCAGTCA GCCAGCTCTA CGCTTGCTGC CCTCTCAAAG 
AAAGTGTCTG AAAGAAGTCA GCAGCAGGTG GGCTCATCTG AGCATCAACC CTCAGCTGGC 
TCCTTTCTCT CTCTGGTTTC CATGACCTCC TCAGCAGCTC TGCTAAAGGA GGTGGCCGCA 
CGAGCAGCAG GGAATCTTCT TGCTGATAAG AAAGAGCCGG CGTTAGTACT GGGAGCCTCC 
GAAGATGTCA AGCCCCTGTT AGACAGGAAC CAGAAGGTGC CCACCCCAAC CCAGGCAATG 
GATCTTCTGC TGCCAACAGT CCCCAAGGGA AGGGCCAAGC CCAGCAGTCA AGCAGGTTCT 
CCTGAGGATG GAGGAAAGCC CTTTCAGTGT CCGGTATGCG GTCTGGTCAT CAAGAGAAAG 
AGCTACTGGA AGAGACATAT GGTGATCCAT ACAGGCTTAA AAAGCCACCA GTGTCCCCTC 
TGTCCCTTTC GCTGTGCTCG TAAAGACAAT CTCAAGTCCC ACATGAAGGT CCATCAGCAC 
CAAGACCGAG GGGAGACGTT CCAGTGTGAG CTCTGCCCCT TCACATCCTC CCGCCACTTT 
AGCCTCAAGC TGCACATGCG TTGCCATCAG CACTTCCCTC GTCCCGACCT CAAAGTCAAA 
GAAGAACCCG GCACAGACAC CGAGGAGGGC GAGGGCTTGC TCATGGGGGA CGGTGGAGCG 
GCTGGTGATC AGGCCATGAA CACAGATGCT TCTACGTCTC CCACTGTGAG CCAACCCGAC 
GCTCGTTTGC TGGCCTCTCC TGCAGAACCC TCAAACCACG TCCAGATCAA GGAGGAACCA 
CAGGAGAGGG ATGTGTCTGT GATGTCTCCC TTTGCCCTGT GTAGGGAGAG GCCGAGCAGC 
AGCAGCAGTT CTCTGGATCT GTCCGGGCTT AAATCTAGTC CACCGGGGCC CGGGCCTACA 
GCTGCTTCAC TCTTCAGCCC AGATATCACC ACCAAGACGG CTACAGACCT CCTCATAAAG 
CTCTCGGCAG CGAACCAGAA GGAGGCCCTA AAGGGTCCAT ACTGTGTCAA GGAAGAGCCC 
CAGGCGGAGG AGCCCCTGAA CTCTCAGAGG GGACCCAGCT ACAGCTTCTG CCAAGAAGGA 
CCTCCAGGAG CCACCAAACC AAGAGAGGAG GGGAGCCCGA TGTTGGGCAA GAGCAGCCTT 
CTCAGCCAGG ACATCAACGT CAAAGTGGCC TCTGAATTGC TTATGAAGCT CTCAGAGAAC 
AACAAAGACG CTTGCGTCCA GAATGTTATT GTGAAGGCGG AGCCTATGGA AGTGGATCCA 
CCCCTTGATC TGGCCTCACC CTCCTCCCAT CTACTGGGCT TTAGCACTTC AGGACCATGT 
GAAAAGAAGG AACCAATGGT CAACCTCCCA GATCATTTGC CCCGCCCCCA GAATGACCTC 
TTCTCGCAGG ACATATCGGT CAAAATGGCC TCCGAGCTGC TTTTCAAACT CTCTGAAAAA 
GTAAGTAAAG CTAATGAAAA CAAAGACAAT GCATCATTCG GTATACACAG CCCTTTCCTT 
GATGACCGCT TTAGACAATC ACCTTTCAGC ACTCGCTCCA AGAGCTCTTC CCCAGCTGAA 
GCCGGATCTT CAAGCAGGGT TCCCCAGCAA GATCCAGAGA AGGTGAGTAG TGAGCCAGGA 
AATGGACTTT CCCAATGGAG ACTCAACGAA CAGCTCTTTC CCTGCCCTGT GTGTGGCAAA 
GTGTTTGGTC GCCAACAGAC TCTCTCCAGG CATCTCTCCT TACACACAGA AGAGAGGAAA 
TACAAGTGTC ATTTGTGTCC ATATGCAGCT AAGTGCCGAG CCAACCTCAA CCAGCACCTC 
ACCATCCACT CAGTCAAACT GGTGAACACA GATGTCGAGC AGATTGTTAC CGCTGTTACC 
AACGATGGCC CAGAGCTCAA AAACTGCCCC TACTACTACA GCTGCCATGT CTGCGGCTTC 
CAGACAGAGT TCAATGCTCA GTTTGTCAGT CATATGTCTC TTCATGTGGA CAAAGAGCAG 
TGGATGTTTT CTCTCTGCTG TAGCACCTGT GAGTATGTGG GTGTGGACGA GGCCGAGATG 
AAAGCTCACA TCAGTGCAGG GCACGCAGGT CTCAATTCTA GAAGCCCTCT CAGTGAGACC 
AAGAGCACTT CCTCCTCTCT GTCAGCCCTC AGCGACTCCC TCAACAGCTC TGAAGGTGGA 
GATGTTGCCC AAGGTAACGA AGAACTAAAA AGCCTTTTGG CTCCGCCTTC ATCTGTGGGA 
AGCCAATCAA GCTTAGGCCC AGGAGCAGAG GAAAAAGCAG AGAAAGGCTT TGAATGCGTC 
TTCTGCAACT TTGTGTGTAA AACCCGACCC ATGTACGAGC GGCACCTACA GATCCACCTG 
ATCACGCGCA TGTTTGAGTG TGACGTCTGC CACAAGTTCA TGAAGACGCC CGAGCAGCTC 
CTGGAGCACA AGAAGTGCCA CACCGTCCCC GCTGGAGGGC TCAAGCCAGC CGCCATGGAG 
GGCCACCTGA AGACACACTA CAAGATGGAG TATCGCTGCC GCATCTGCCA AGCTGTGTGG 
GCCAATCAGG CAGAGCTGGA AAGGCACACG CGCGCTCACC GCCTGGGCAA CCACTATAAG 
TGTGAGCAGT GCGGCTACCT CTCCAAAACA GCCAACAAGC TCATCGAGCA TGTGCGCGTG 
CACACCGGCG AGCGGCCTTT CCACTGCGAC CGCTGCAGCT ACAGCTGCAA ACGCAAGGAC 
AATCTCAACC TCCACAAGCG GCTCAAGCAC GCACCGAGAC AGACCTTCGG TTGCGCCCAG 
TGCCCATTCA CCACCACTCA TCCGTTCGTC TACAGCCGCC ATGTCAAAAA GCACCAAGGC 
GGGGAGGGAA GCGCCGAGGA AGGCGAGGCT CGGCCAGGTA CGCCCCATCC GGGCCTTCTG 
GAGCGAGGTG GGAGCAGCAG CGGGAGCGGG GACAGTGGGA GCACCAGTCC GCTCATGAAC 
CTGTCGGCCT CTCAAGCCTT GCAGTCCGTC GCCCTGTCAA TCACCAGCAA GCAGCAAGAT 
GGCACGCCAA CCGCGCGCTA CCTCTCAGCC AATGGCACGT TGTCCACACT GGCGGCTCGC 
TACTTTGACC AGTACAGGAA CTGTGACCTG GCTCACCTCG TCCCCCTCAC CACGCTCTTT 
ACCACCCGAC GCTTCACATT CCACAGCCCC TCGCCCTCAA ACCCTTCGCC GCCCTTCTCT 
GCATTCTGGT CCAACTCATC CCCGCAGTCA CCACCCTCTC CCACATCACT CAAACACTCC 
TTCCTGGCAT ATCTGGGACT GACAGAGAGA GCGAAGACCA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001919201.4
CDS	413..4657
Translation	MPRRKQEQPKRLPSHVDTVEDGGPESGSVEEDSWFGNTSDSPSESSYGDVQDELRVSPDNVPRPDHDTSAESSLGCPTPVQRASLELLGLDSGVLSTQDTLSSVVSSLYGDSSQALGKPLSSNLRRLLEAGSLKLEGGELAGRGTGRGQESPPVSLVLSPSSHHAQQLSALARRLANSSNNSGNSNSASPASVATVKQEPCDPYGPSNGPNSGSSGFVWVGVADKWPSTSCSAPSGSSLSPDSAIQKLKAAANAVLQDKNAVASSTSASSSSSSSSSSSSVTSSSMAALGTRSDDAVRFEPFASPFSPQSASSTLAALSKKVSERSQQQVGSSEHQPSAGSFLSLVSMTSSAALLKEVAARAAGNLLADKKEPALVLGASEDVKPLLDRNQKVPTPTQAMDLLLPTVPKGRAKPSSQAGSPEDGGKPFQCPVCGLVIKRKSYWKRHMVIHTGLKSHQCPLCPFRCARKDNLKSHMKVHQHQDRGETFQCELCPFTSSRHFSLKLHMRCHQHFPRPDLKVKEEPGTDTEEGEGLLMGDGGAAGDQAMNTDASTSPTVSQPDARLLASPAEPSNHVQIKEEPQERDVSVMSPFALCRERPSSSSSSLDLSGLKSSPPGPGPTAASLFSPDITTKTATDLLIKLSAANQKEALKGPYCVKEEPQAEEPLNSQRGPSYSFCQEGPPGATKPREEGSPMLGKSSLLSQDINVKVASELLMKLSENNKDACVQNVIVKAEPMEVDPPLDLASPSSHLLGFSTSGPCEKKEPMVNLPDHLPRPQNDLFSQDISVKMASELLFKLSEKVSKANENKDNASFGIHSPFLDDRFRQSPFSTRSKSSSPAEAGSSSRVPQQDPEKVSSEPGNGLSQWRLNEQLFPCPVCGKVFGRQQTLSRHLSLHTEERKYKCHLCPYAAKCRANLNQHLTIHSVKLVNTDVEQIVTAVTNDGPELKNCPYYYSCHVCGFQTEFNAQFVSHMSLHVDKEQWMFSLCCSTCEYVGVDEAEMKAHISAGHAGLNSRSPLSETKSTSSSLSALSDSLNSSEGGDVAQGNEELKSLLAPPSSVGSQSSLGPGAEEKAEKGFECVFCNFVCKTRPMYERHLQIHLITRMFECDVCHKFMKTPEQLLEHKKCHTVPAGGLKPAAMEGHLKTHYKMEYRCRICQAVWANQAELERHTRAHRLGNHYKCEQCGYLSKTANKLIEHVRVHTGERPFHCDRCSYSCKRKDNLNLHKRLKHAPRQTFGCAQCPFTTTHPFVYSRHVKKHQGGEGSAEEGEARPGTPHPGLLERGGSSSGSGDSGSTSPLMNLSASQALQSVALSITSKQQDGTPTARYLSANGTLSTLAARYFDQYRNCDLAHLVPLTTLFTTRRFTFHSPSPSNPSPPFSAFWSNSSPQSPPSPTSLKHSFLAYLGLTERAKTI

Target ORF information:

RefSeq Version	XM_001919166.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zinc finger protein 827 (znf827), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001919166.7

ORF Insert Sequence:

ATGCCAAGGA GGAAACAGGA GCAACCCAAG CGCCTGCCTT CACATGTGGA CACAGTAGAG 
GATGGAGGGC CAGAGAGTGG CTCAGTAGAG GAGGACAGCT GGTTTGGGAA TACCTCCGAC 
AGCCCCTCGG AATCGTCCTA CGGTGATGTG CAAGATGAGC TTCGGGTTTC TCCAGACAAC 
GTTCCCCGAC CGGACCACGA CACCTCAGCT GAGAGCTCCC TGGGCTGCCC GACGCCAGTT 
CAGCGGGCCT CTCTGGAGCT TCTGGGCCTG GACAGTGGGG TCCTTTCGAC CCAGGACACG 
CTTTCCTCTG TGGTGTCCTC TCTTTATGGA GACTCTAGCC AGGCTCTGGG TAAGCCACTC 
AGCAGTAATC TGCGCCGCCT CCTGGAGGCT GGCTCACTGA AGCTTGAGGG AGGGGAGCTG 
GCTGGACGTG GAACGGGTAG AGGACAAGAG TCACCCCCGG TCAGCCTGGT ACTATCTCCG 
TCTTCACATC ACGCACAGCA GCTGAGTGCC CTGGCGAGGA GGTTGGCAAA TAGCAGCAAC 
AACAGCGGAA ATTCAAACTC TGCGTCACCT GCATCTGTAG CAACTGTCAA GCAGGAGCCC 
TGTGACCCAT ACGGCCCCAG CAACGGCCCT AACTCAGGCA GCAGTGGCTT TGTATGGGTA 
GGCGTCGCAG ATAAATGGCC ATCGACAAGT TGCTCAGCAC CCAGTGGCAG TAGCCTCTCA 
CCAGACTCAG CAATTCAGAA GTTAAAGGCA GCCGCCAACG CCGTGCTCCA AGACAAGAAC 
GCGGTGGCCT CCTCCACCTC GGCATCTTCA TCCTCCTCGT CCTCCTCCTC ATCCTCTTCA 
GTCACCTCCT CCTCAATGGC AGCCCTGGGC ACTCGCTCTG ATGACGCAGT GCGCTTTGAA 
CCATTCGCTT CACCCTTTAG CCCGCAGTCA GCCAGCTCTA CGCTTGCTGC CCTCTCAAAG 
AAAGTGTCTG AAAGAAGTCA GCAGCAGGTG GGCTCATCTG AGCATCAACC CTCAGCTGGC 
TCCTTTCTCT CTCTGGTTTC CATGACCTCC TCAGCAGCTC TGCTAAAGGA GGTGGCCGCA 
CGAGCAGCAG GGAATCTTCT TGCTGATAAG AAAGAGCCGG CGTTAGTACT GGGAGCCTCC 
GAAGATGTCA AGCCCCTGTT AGACAGGAAC CAGAAGGTGC CCACCCCAAC CCAGGCAATG 
GATCTTCTGC TGCCAACAGT CCCCAAGGGA AGGGCCAAGC CCAGCAGTCA AGCAGGTTCT 
CCTGAGGATG GAGGAAAGCC CTTTCAGTGT CCGGTATGCG GTCTGGTCAT CAAGAGAAAG 
AGCTACTGGA AGAGACATAT GGTGATCCAT ACAGGCTTAA AAAGCCACCA GTGTCCCCTC 
TGTCCCTTTC GCTGTGCTCG TAAAGACAAT CTCAAGTCCC ACATGAAGGT CCATCAGCAC 
CAAGACCGAG GGGAGACGTT CCAGTGTGAG CTCTGCCCCT TCACATCCTC CCGCCACTTT 
AGCCTCAAGC TGCACATGCG TTGCCATCAG CACTTCCCTC GTCCCGACCT CAAAGTCAAA 
GAAGAACCCG GCACAGACAC CGAGGAGGGC GAGGGCTTGC TCATGGGGGA CGGTGGAGCG 
GCTGGTGATC AGGCCATGAA CACAGATGCT TCTACGTCTC CCACTGTGAG CCAACCCGAC 
GCTCGTTTGC TGGCCTCTCC TGCAGAACCC TCAAACCACG TCCAGATCAA GGAGGAACCA 
CAGGAGAGGG ATGTGTCTGT GATGTCTCCC TTTGCCCTGT GTAGGGAGAG GCCGAGCAGC 
AGCAGCAGTT CTCTGGATCT GTCCGGGCTT AAATCTAGTC CACCGGGGCC CGGGCCTACA 
GCTGCTTCAC TCTTCAGCCC AGATATCACC ACCAAGACGG CTACAGACCT CCTCATAAAG 
CTCTCGGCAG CGAACCAGAA GGAGGCCCTA AAGGGTCCAT ACTGTGTCAA GGAAGAGCCC 
CAGGCGGAGG AGCCCCTGAA CTCTCAGAGG GGACCCAGCT ACAGCTTCTG CCAAGAAGGA 
CCTCCAGGAG CCACCAAACC AAGAGAGGAG GGGAGCCCGA TGTTGGGCAA GAGCAGCCTT 
CTCAGCCAGG ACATCAACGT CAAAGTGGCC TCTGAATTGC TTATGAAGCT CTCAGAGAAC 
AACAAAGACG CTTGCGTCCA GAATGTTATT GTGAAGGCGG AGCCTATGGA AGTGGATCCA 
CCCCTTGATC TGGCCTCACC CTCCTCCCAT CTACTGGGCT TTAGCACTTC AGGACCATGT 
GAAAAGAAGG AACCAATGGT CAACCTCCCA GATCATTTGC CCCGCCCCCA GAATGACCTC 
TTCTCGCAGG ACATATCGGT CAAAATGGCC TCCGAGCTGC TTTTCAAACT CTCTGAAAAA 
GTAAGTAAAG CTAATGAAAA CAAAGACAAT GCATCATTCG GTATACACAG CCCTTTCCTT 
GATGACCGCT TTAGACAATC ACCTTTCAGC ACTCGCTCCA AGAGCTCTTC CCCAGCTGAA 
GCCGGATCTT CAAGCAGGGT TCCCCAGCAA GATCCAGAGA AGGTGAGTAG TGAGCCAGGA 
AATGGACTTT CCCAATGGAG ACTCAACGAA CAGCTCTTTC CCTGCCCTGT GTGTGGCAAA 
GTGTTTGGTC GCCAACAGAC TCTCTCCAGG CATCTCTCCT TACACACAGA AGAGAGGAAA 
TACAAGTGTC ATTTGTGTCC ATATGCAGCT AAGTGCCGAG CCAACCTCAA CCAGCACCTC 
ACCATCCACT CAGTCAAACT GGTGAACACA GATGTCGAGC AGATTGTTAC CGCTGTTACC 
AACGATGGCC CAGAGCTCAA AAACTGCCCC TACTACTACA GCTGCCATGT CTGCGGCTTC 
CAGACAGAGT TCAATGCTCA GTTTGTCAGT CATATGTCTC TTCATGTGGA CAAAGAGCAG 
TGGATGTTTT CTCTCTGCTG TAGCACCTGT GAGTATGTGG GTGTGGACGA GGCCGAGATG 
AAAGCTCACA TCAGTGCAGG GCACGCAGGT CTCAATTCTA GAAGCCCTCT CAGTGAGACC 
AAGAGCACTT CCTCCTCTCT GTCAGCCCTC AGCGACTCCC TCAACAGCTC TGAAGGTGGA 
GATGTTGCCC AAGGTAACGA AGAACTAAAA AGCCTTTTGG CTCCGCCTTC ATCTGTGGGA 
AGCCAATCAA GCTTAGGCCC AGGAGCAGAG GAAAAAGCAG AGAAAGGCTT TGAATGCGTC 
TTCTGCAACT TTGTGTGTAA AACCCGACCC ATGTACGAGC GGCACCTACA GATCCACCTG 
ATCACGCGCA TGTTTGAGTG TGACGTCTGC CACAAGTTCA TGAAGACGCC CGAGCAGCTC 
CTGGAGCACA AGAAGTGCCA CACCGTCCCC GCTGGAGGGC TCAAGCCAGC CGCCATGGAG 
GGCCACCTGA AGACACACTA CAAGATGGAG TATCGCTGCC GCATCTGCCA AGCTGTGTGG 
GCCAATCAGG CAGAGCTGGA AAGGCACACG CGCGCTCACC GCCTGGGCAA CCACTATAAG 
TGTGAGCAGT GCGGCTACCT CTCCAAAACA GCCAACAAGC TCATCGAGCA TGTGCGCGTG 
CACACCGGCG AGCGGCCTTT CCACTGCGAC CGCTGCAGCT ACAGCTGCAA ACGCAAGGAC 
AATCTCAACC TCCACAAGCG GCTCAAGCAC GCACCGAGAC AGACCTTCGG TTGCGCCCAG 
TGCCCATTCA CCACCACTCA TCCGTTCGTC TACAGCCGCC ATGTCAAAAA GCACCAAGGC 
GGGGAGGGAA GCGCCGAGGA AGGCGAGGCT CGGCCAGGTA CGCCCCATCC GGGCCTTCTG 
GAGCGAGGTG GGAGCAGCAG CGGGAGCGGG GACAGTGGGA GCACCAGTCC GCTCATGAAC 
CTGTCGGCCT CTCAAGCCTT GCAGTCCGTC GCCCTGTCAA TCACCAGCAA GCAGCAAGAT 
GGCACGCCAA CCGCGCGCTA CCTCTCAGCC AATGGCACGT TGTCCACACT GGCGGCTCGC 
TACTTTGACC AGTACAGGAA CTGTGACCTG GCTCACCTCG TCCCCCTCAC CACGCTCTTT 
ACCACCCGAC GCTTCACATT CCACAGCCCC TCGCCCTCAA ACCCTTCGCC GCCCTTCTCT 
GCATTCTGGT CCAACTCATC CCCGCAGTCA CCACCCTCTC CCACATCACT CAAACACTCC 
TTCCTGGCAT ATCTGGGACT GACAGAGAGA GCGAAGACCA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf827 ( XM_001919166.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001919201.4 Danio rerio zinc finger protein 827 (znf827), transcript variant X2, mRNA.

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CloneID	ODa05909
Clone ID Related Accession (Same CDS sequence)	XM_001919166.7 , XM_001919166.6
Accession Version	XM_001919166.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	zinc finger protein 827 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001919166.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GGAAACAGGA GCAACCCAAG CGCCTGCCTT CACATGTGGA CACAGTAGAG 
GATGGAGGGC CAGAGAGTGG CTCAGTAGAG GAGGACAGCT GGTTTGGGAA TACCTCCGAC 
AGCCCCTCGG AATCGTCCTA CGGTGATGTG CAAGATGAGC TTCGGGTTTC TCCAGACAAC 
GTTCCCCGAC CGGACCACGA CACCTCAGCT GAGAGCTCCC TGGGCTGCCC GACGCCAGTT 
CAGCGGGCCT CTCTGGAGCT TCTGGGCCTG GACAGTGGGG TCCTTTCGAC CCAGGACACG 
CTTTCCTCTG TGGTGTCCTC TCTTTATGGA GACTCTAGCC AGGCTCTGGG TAAGCCACTC 
AGCAGTAATC TGCGCCGCCT CCTGGAGGCT GGCTCACTGA AGCTTGAGGG AGGGGAGCTG 
GCTGGACGTG GAACGGGTAG AGGACAAGAG TCACCCCCGG TCAGCCTGGT ACTATCTCCG 
TCTTCACATC ACGCACAGCA GCTGAGTGCC CTGGCGAGGA GGTTGGCAAA TAGCAGCAAC 
AACAGCGGAA ATTCAAACTC TGCGTCACCT GCATCTGTAG CAACTGTCAA GCAGGAGCCC 
TGTGACCCAT ACGGCCCCAG CAACGGCCCT AACTCAGGCA GCAGTGGCTT TGTATGGGTA 
GGCGTCGCAG ATAAATGGCC ATCGACAAGT TGCTCAGCAC CCAGTGGCAG TAGCCTCTCA 
CCAGACTCAG CAATTCAGAA GTTAAAGGCA GCCGCCAACG CCGTGCTCCA AGACAAGAAC 
GCGGTGGCCT CCTCCACCTC GGCATCTTCA TCCTCCTCGT CCTCCTCCTC ATCCTCTTCA 
GTCACCTCCT CCTCAATGGC AGCCCTGGGC ACTCGCTCTG ATGACGCAGT GCGCTTTGAA 
CCATTCGCTT CACCCTTTAG CCCGCAGTCA GCCAGCTCTA CGCTTGCTGC CCTCTCAAAG 
AAAGTGTCTG AAAGAAGTCA GCAGCAGGTG GGCTCATCTG AGCATCAACC CTCAGCTGGC 
TCCTTTCTCT CTCTGGTTTC CATGACCTCC TCAGCAGCTC TGCTAAAGGA GGTGGCCGCA 
CGAGCAGCAG GGAATCTTCT TGCTGATAAG AAAGAGCCGG CGTTAGTACT GGGAGCCTCC 
GAAGATGTCA AGCCCCTGTT AGACAGGAAC CAGAAGGTGC CCACCCCAAC CCAGGCAATG 
GATCTTCTGC TGCCAACAGT CCCCAAGGGA AGGGCCAAGC CCAGCAGTCA AGCAGGTTCT 
CCTGAGGATG GAGGAAAGCC CTTTCAGTGT CCGGTATGCG GTCTGGTCAT CAAGAGAAAG 
AGCTACTGGA AGAGACATAT GGTGATCCAT ACAGGCTTAA AAAGCCACCA GTGTCCCCTC 
TGTCCCTTTC GCTGTGCTCG TAAAGACAAT CTCAAGTCCC ACATGAAGGT CCATCAGCAC 
CAAGACCGAG GGGAGACGTT CCAGTGTGAG CTCTGCCCCT TCACATCCTC CCGCCACTTT 
AGCCTCAAGC TGCACATGCG TTGCCATCAG CACTTCCCTC GTCCCGACCT CAAAGTCAAA 
GAAGAACCCG GCACAGACAC CGAGGAGGGC GAGGGCTTGC TCATGGGGGA CGGTGGAGCG 
GCTGGTGATC AGGCCATGAA CACAGATGCT TCTACGTCTC CCACTGTGAG CCAACCCGAC 
GCTCGTTTGC TGGCCTCTCC TGCAGAACCC TCAAACCACG TCCAGATCAA GGAGGAACCA 
CAGGAGAGGG ATGTGTCTGT GATGTCTCCC TTTGCCCTGT GTAGGGAGAG GCCGAGCAGC 
AGCAGCAGTT CTCTGGATCT GTCCGGGCTT AAATCTAGTC CACCGGGGCC CGGGCCTACA 
GCTGCTTCAC TCTTCAGCCC AGATATCACC ACCAAGACGG CTACAGACCT CCTCATAAAG 
CTCTCGGCAG CGAACCAGAA GGAGGCCCTA AAGGGTCCAT ACTGTGTCAA GGAAGAGCCC 
CAGGCGGAGG AGCCCCTGAA CTCTCAGAGG GGACCCAGCT ACAGCTTCTG CCAAGAAGGA 
CCTCCAGGAG CCACCAAACC AAGAGAGGAG GGGAGCCCGA TGTTGGGCAA GAGCAGCCTT 
CTCAGCCAGG ACATCAACGT CAAAGTGGCC TCTGAATTGC TTATGAAGCT CTCAGAGAAC 
AACAAAGACG CTTGCGTCCA GAATGTTATT GTGAAGGCGG AGCCTATGGA AGTGGATCCA 
CCCCTTGATC TGGCCTCACC CTCCTCCCAT CTACTGGGCT TTAGCACTTC AGGACCATGT 
GAAAAGAAGG AACCAATGGT CAACCTCCCA GATCATTTGC CCCGCCCCCA GAATGACCTC 
TTCTCGCAGG ACATATCGGT CAAAATGGCC TCCGAGCTGC TTTTCAAACT CTCTGAAAAA 
GTAAGTAAAG CTAATGAAAA CAAAGACAAT GCATCATTCG GTATACACAG CCCTTTCCTT 
GATGACCGCT TTAGACAATC ACCTTTCAGC ACTCGCTCCA AGAGCTCTTC CCCAGCTGAA 
GCCGGATCTT CAAGCAGGGT TCCCCAGCAA GATCCAGAGA AGGTGAGTAG TGAGCCAGGA 
AATGGACTTT CCCAATGGAG ACTCAACGAA CAGCTCTTTC CCTGCCCTGT GTGTGGCAAA 
GTGTTTGGTC GCCAACAGAC TCTCTCCAGG CATCTCTCCT TACACACAGA AGAGAGGAAA 
TACAAGTGTC ATTTGTGTCC ATATGCAGCT AAGTGCCGAG CCAACCTCAA CCAGCACCTC 
ACCATCCACT CAGTCAAACT GGTGAACACA GATGTCGAGC AGATTGTTAC CGCTGTTACC 
AACGATGGCC CAGAGCTCAA AAACTGCCCC TACTACTACA GCTGCCATGT CTGCGGCTTC 
CAGACAGAGT TCAATGCTCA GTTTGTCAGT CATATGTCTC TTCATGTGGA CAAAGAGCAG 
TGGATGTTTT CTCTCTGCTG TAGCACCTGT GAGTATGTGG GTGTGGACGA GGCCGAGATG 
AAAGCTCACA TCAGTGCAGG GCACGCAGGT CTCAATTCTA GAAGCCCTCT CAGTGAGACC 
AAGAGCACTT CCTCCTCTCT GTCAGCCCTC AGCGACTCCC TCAACAGCTC TGAAGGTGGA 
GATGTTGCCC AAGGTAACGA AGAACTAAAA AGCCTTTTGG CTCCGCCTTC ATCTGTGGGA 
AGCCAATCAA GCTTAGGCCC AGGAGCAGAG GAAAAAGCAG AGAAAGGCTT TGAATGCGTC 
TTCTGCAACT TTGTGTGTAA AACCCGACCC ATGTACGAGC GGCACCTACA GATCCACCTG 
ATCACGCGCA TGTTTGAGTG TGACGTCTGC CACAAGTTCA TGAAGACGCC CGAGCAGCTC 
CTGGAGCACA AGAAGTGCCA CACCGTCCCC GCTGGAGGGC TCAAGCCAGC CGCCATGGAG 
GGCCACCTGA AGACACACTA CAAGATGGAG TATCGCTGCC GCATCTGCCA AGCTGTGTGG 
GCCAATCAGG CAGAGCTGGA AAGGCACACG CGCGCTCACC GCCTGGGCAA CCACTATAAG 
TGTGAGCAGT GCGGCTACCT CTCCAAAACA GCCAACAAGC TCATCGAGCA TGTGCGCGTG 
CACACCGGCG AGCGGCCTTT CCACTGCGAC CGCTGCAGCT ACAGCTGCAA ACGCAAGGAC 
AATCTCAACC TCCACAAGCG GCTCAAGCAC GCACCGAGAC AGACCTTCGG TTGCGCCCAG 
TGCCCATTCA CCACCACTCA TCCGTTCGTC TACAGCCGCC ATGTCAAAAA GCACCAAGGC 
GGGGAGGGAA GCGCCGAGGA AGGCGAGGCT CGGCCAGGTA CGCCCCATCC GGGCCTTCTG 
GAGCGAGGTG GGAGCAGCAG CGGGAGCGGG GACAGTGGGA GCACCAGTCC GCTCATGAAC 
CTGTCGGCCT CTCAAGCCTT GCAGTCCGTC GCCCTGTCAA TCACCAGCAA GCAGCAAGAT 
GGCACGCCAA CCGCGCGCTA CCTCTCAGCC AATGGCACGT TGTCCACACT GGCGGCTCGC 
TACTTTGACC AGTACAGGAA CTGTGACCTG GCTCACCTCG TCCCCCTCAC CACGCTCTTT 
ACCACCCGAC GCTTCACATT CCACAGCCCC TCGCCCTCAA ACCCTTCGCC GCCCTTCTCT 
GCATTCTGGT CCAACTCATC CCCGCAGTCA CCACCCTCTC CCACATCACT CAAACACTCC 
TTCCTGGCAT ATCTGGGACT GACAGAGAGA GCGAAGACCA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001919201.4
CDS	856..5100
Translation	MPRRKQEQPKRLPSHVDTVEDGGPESGSVEEDSWFGNTSDSPSESSYGDVQDELRVSPDNVPRPDHDTSAESSLGCPTPVQRASLELLGLDSGVLSTQDTLSSVVSSLYGDSSQALGKPLSSNLRRLLEAGSLKLEGGELAGRGTGRGQESPPVSLVLSPSSHHAQQLSALARRLANSSNNSGNSNSASPASVATVKQEPCDPYGPSNGPNSGSSGFVWVGVADKWPSTSCSAPSGSSLSPDSAIQKLKAAANAVLQDKNAVASSTSASSSSSSSSSSSSVTSSSMAALGTRSDDAVRFEPFASPFSPQSASSTLAALSKKVSERSQQQVGSSEHQPSAGSFLSLVSMTSSAALLKEVAARAAGNLLADKKEPALVLGASEDVKPLLDRNQKVPTPTQAMDLLLPTVPKGRAKPSSQAGSPEDGGKPFQCPVCGLVIKRKSYWKRHMVIHTGLKSHQCPLCPFRCARKDNLKSHMKVHQHQDRGETFQCELCPFTSSRHFSLKLHMRCHQHFPRPDLKVKEEPGTDTEEGEGLLMGDGGAAGDQAMNTDASTSPTVSQPDARLLASPAEPSNHVQIKEEPQERDVSVMSPFALCRERPSSSSSSLDLSGLKSSPPGPGPTAASLFSPDITTKTATDLLIKLSAANQKEALKGPYCVKEEPQAEEPLNSQRGPSYSFCQEGPPGATKPREEGSPMLGKSSLLSQDINVKVASELLMKLSENNKDACVQNVIVKAEPMEVDPPLDLASPSSHLLGFSTSGPCEKKEPMVNLPDHLPRPQNDLFSQDISVKMASELLFKLSEKVSKANENKDNASFGIHSPFLDDRFRQSPFSTRSKSSSPAEAGSSSRVPQQDPEKVSSEPGNGLSQWRLNEQLFPCPVCGKVFGRQQTLSRHLSLHTEERKYKCHLCPYAAKCRANLNQHLTIHSVKLVNTDVEQIVTAVTNDGPELKNCPYYYSCHVCGFQTEFNAQFVSHMSLHVDKEQWMFSLCCSTCEYVGVDEAEMKAHISAGHAGLNSRSPLSETKSTSSSLSALSDSLNSSEGGDVAQGNEELKSLLAPPSSVGSQSSLGPGAEEKAEKGFECVFCNFVCKTRPMYERHLQIHLITRMFECDVCHKFMKTPEQLLEHKKCHTVPAGGLKPAAMEGHLKTHYKMEYRCRICQAVWANQAELERHTRAHRLGNHYKCEQCGYLSKTANKLIEHVRVHTGERPFHCDRCSYSCKRKDNLNLHKRLKHAPRQTFGCAQCPFTTTHPFVYSRHVKKHQGGEGSAEEGEARPGTPHPGLLERGGSSSGSGDSGSTSPLMNLSASQALQSVALSITSKQQDGTPTARYLSANGTLSTLAARYFDQYRNCDLAHLVPLTTLFTTRRFTFHSPSPSNPSPPFSAFWSNSSPQSPPSPTSLKHSFLAYLGLTERAKTI

Target ORF information:

RefSeq Version	XM_001919166.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio zinc finger protein 827 (znf827), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001919166.6

ORF Insert Sequence:

ATGCCAAGGA GGAAACAGGA GCAACCCAAG CGCCTGCCTT CACATGTGGA CACAGTAGAG 
GATGGAGGGC CAGAGAGTGG CTCAGTAGAG GAGGACAGCT GGTTTGGGAA TACCTCCGAC 
AGCCCCTCGG AATCGTCCTA CGGTGATGTG CAAGATGAGC TTCGGGTTTC TCCAGACAAC 
GTTCCCCGAC CGGACCACGA CACCTCAGCT GAGAGCTCCC TGGGCTGCCC GACGCCAGTT 
CAGCGGGCCT CTCTGGAGCT TCTGGGCCTG GACAGTGGGG TCCTTTCGAC CCAGGACACG 
CTTTCCTCTG TGGTGTCCTC TCTTTATGGA GACTCTAGCC AGGCTCTGGG TAAGCCACTC 
AGCAGTAATC TGCGCCGCCT CCTGGAGGCT GGCTCACTGA AGCTTGAGGG AGGGGAGCTG 
GCTGGACGTG GAACGGGTAG AGGACAAGAG TCACCCCCGG TCAGCCTGGT ACTATCTCCG 
TCTTCACATC ACGCACAGCA GCTGAGTGCC CTGGCGAGGA GGTTGGCAAA TAGCAGCAAC 
AACAGCGGAA ATTCAAACTC TGCGTCACCT GCATCTGTAG CAACTGTCAA GCAGGAGCCC 
TGTGACCCAT ACGGCCCCAG CAACGGCCCT AACTCAGGCA GCAGTGGCTT TGTATGGGTA 
GGCGTCGCAG ATAAATGGCC ATCGACAAGT TGCTCAGCAC CCAGTGGCAG TAGCCTCTCA 
CCAGACTCAG CAATTCAGAA GTTAAAGGCA GCCGCCAACG CCGTGCTCCA AGACAAGAAC 
GCGGTGGCCT CCTCCACCTC GGCATCTTCA TCCTCCTCGT CCTCCTCCTC ATCCTCTTCA 
GTCACCTCCT CCTCAATGGC AGCCCTGGGC ACTCGCTCTG ATGACGCAGT GCGCTTTGAA 
CCATTCGCTT CACCCTTTAG CCCGCAGTCA GCCAGCTCTA CGCTTGCTGC CCTCTCAAAG 
AAAGTGTCTG AAAGAAGTCA GCAGCAGGTG GGCTCATCTG AGCATCAACC CTCAGCTGGC 
TCCTTTCTCT CTCTGGTTTC CATGACCTCC TCAGCAGCTC TGCTAAAGGA GGTGGCCGCA 
CGAGCAGCAG GGAATCTTCT TGCTGATAAG AAAGAGCCGG CGTTAGTACT GGGAGCCTCC 
GAAGATGTCA AGCCCCTGTT AGACAGGAAC CAGAAGGTGC CCACCCCAAC CCAGGCAATG 
GATCTTCTGC TGCCAACAGT CCCCAAGGGA AGGGCCAAGC CCAGCAGTCA AGCAGGTTCT 
CCTGAGGATG GAGGAAAGCC CTTTCAGTGT CCGGTATGCG GTCTGGTCAT CAAGAGAAAG 
AGCTACTGGA AGAGACATAT GGTGATCCAT ACAGGCTTAA AAAGCCACCA GTGTCCCCTC 
TGTCCCTTTC GCTGTGCTCG TAAAGACAAT CTCAAGTCCC ACATGAAGGT CCATCAGCAC 
CAAGACCGAG GGGAGACGTT CCAGTGTGAG CTCTGCCCCT TCACATCCTC CCGCCACTTT 
AGCCTCAAGC TGCACATGCG TTGCCATCAG CACTTCCCTC GTCCCGACCT CAAAGTCAAA 
GAAGAACCCG GCACAGACAC CGAGGAGGGC GAGGGCTTGC TCATGGGGGA CGGTGGAGCG 
GCTGGTGATC AGGCCATGAA CACAGATGCT TCTACGTCTC CCACTGTGAG CCAACCCGAC 
GCTCGTTTGC TGGCCTCTCC TGCAGAACCC TCAAACCACG TCCAGATCAA GGAGGAACCA 
CAGGAGAGGG ATGTGTCTGT GATGTCTCCC TTTGCCCTGT GTAGGGAGAG GCCGAGCAGC 
AGCAGCAGTT CTCTGGATCT GTCCGGGCTT AAATCTAGTC CACCGGGGCC CGGGCCTACA 
GCTGCTTCAC TCTTCAGCCC AGATATCACC ACCAAGACGG CTACAGACCT CCTCATAAAG 
CTCTCGGCAG CGAACCAGAA GGAGGCCCTA AAGGGTCCAT ACTGTGTCAA GGAAGAGCCC 
CAGGCGGAGG AGCCCCTGAA CTCTCAGAGG GGACCCAGCT ACAGCTTCTG CCAAGAAGGA 
CCTCCAGGAG CCACCAAACC AAGAGAGGAG GGGAGCCCGA TGTTGGGCAA GAGCAGCCTT 
CTCAGCCAGG ACATCAACGT CAAAGTGGCC TCTGAATTGC TTATGAAGCT CTCAGAGAAC 
AACAAAGACG CTTGCGTCCA GAATGTTATT GTGAAGGCGG AGCCTATGGA AGTGGATCCA 
CCCCTTGATC TGGCCTCACC CTCCTCCCAT CTACTGGGCT TTAGCACTTC AGGACCATGT 
GAAAAGAAGG AACCAATGGT CAACCTCCCA GATCATTTGC CCCGCCCCCA GAATGACCTC 
TTCTCGCAGG ACATATCGGT CAAAATGGCC TCCGAGCTGC TTTTCAAACT CTCTGAAAAA 
GTAAGTAAAG CTAATGAAAA CAAAGACAAT GCATCATTCG GTATACACAG CCCTTTCCTT 
GATGACCGCT TTAGACAATC ACCTTTCAGC ACTCGCTCCA AGAGCTCTTC CCCAGCTGAA 
GCCGGATCTT CAAGCAGGGT TCCCCAGCAA GATCCAGAGA AGGTGAGTAG TGAGCCAGGA 
AATGGACTTT CCCAATGGAG ACTCAACGAA CAGCTCTTTC CCTGCCCTGT GTGTGGCAAA 
GTGTTTGGTC GCCAACAGAC TCTCTCCAGG CATCTCTCCT TACACACAGA AGAGAGGAAA 
TACAAGTGTC ATTTGTGTCC ATATGCAGCT AAGTGCCGAG CCAACCTCAA CCAGCACCTC 
ACCATCCACT CAGTCAAACT GGTGAACACA GATGTCGAGC AGATTGTTAC CGCTGTTACC 
AACGATGGCC CAGAGCTCAA AAACTGCCCC TACTACTACA GCTGCCATGT CTGCGGCTTC 
CAGACAGAGT TCAATGCTCA GTTTGTCAGT CATATGTCTC TTCATGTGGA CAAAGAGCAG 
TGGATGTTTT CTCTCTGCTG TAGCACCTGT GAGTATGTGG GTGTGGACGA GGCCGAGATG 
AAAGCTCACA TCAGTGCAGG GCACGCAGGT CTCAATTCTA GAAGCCCTCT CAGTGAGACC 
AAGAGCACTT CCTCCTCTCT GTCAGCCCTC AGCGACTCCC TCAACAGCTC TGAAGGTGGA 
GATGTTGCCC AAGGTAACGA AGAACTAAAA AGCCTTTTGG CTCCGCCTTC ATCTGTGGGA 
AGCCAATCAA GCTTAGGCCC AGGAGCAGAG GAAAAAGCAG AGAAAGGCTT TGAATGCGTC 
TTCTGCAACT TTGTGTGTAA AACCCGACCC ATGTACGAGC GGCACCTACA GATCCACCTG 
ATCACGCGCA TGTTTGAGTG TGACGTCTGC CACAAGTTCA TGAAGACGCC CGAGCAGCTC 
CTGGAGCACA AGAAGTGCCA CACCGTCCCC GCTGGAGGGC TCAAGCCAGC CGCCATGGAG 
GGCCACCTGA AGACACACTA CAAGATGGAG TATCGCTGCC GCATCTGCCA AGCTGTGTGG 
GCCAATCAGG CAGAGCTGGA AAGGCACACG CGCGCTCACC GCCTGGGCAA CCACTATAAG 
TGTGAGCAGT GCGGCTACCT CTCCAAAACA GCCAACAAGC TCATCGAGCA TGTGCGCGTG 
CACACCGGCG AGCGGCCTTT CCACTGCGAC CGCTGCAGCT ACAGCTGCAA ACGCAAGGAC 
AATCTCAACC TCCACAAGCG GCTCAAGCAC GCACCGAGAC AGACCTTCGG TTGCGCCCAG 
TGCCCATTCA CCACCACTCA TCCGTTCGTC TACAGCCGCC ATGTCAAAAA GCACCAAGGC 
GGGGAGGGAA GCGCCGAGGA AGGCGAGGCT CGGCCAGGTA CGCCCCATCC GGGCCTTCTG 
GAGCGAGGTG GGAGCAGCAG CGGGAGCGGG GACAGTGGGA GCACCAGTCC GCTCATGAAC 
CTGTCGGCCT CTCAAGCCTT GCAGTCCGTC GCCCTGTCAA TCACCAGCAA GCAGCAAGAT 
GGCACGCCAA CCGCGCGCTA CCTCTCAGCC AATGGCACGT TGTCCACACT GGCGGCTCGC 
TACTTTGACC AGTACAGGAA CTGTGACCTG GCTCACCTCG TCCCCCTCAC CACGCTCTTT 
ACCACCCGAC GCTTCACATT CCACAGCCCC TCGCCCTCAA ACCCTTCGCC GCCCTTCTCT 
GCATTCTGGT CCAACTCATC CCCGCAGTCA CCACCCTCTC CCACATCACT CAAACACTCC 
TTCCTGGCAT ATCTGGGACT GACAGAGAGA GCGAAGACCA TTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf827 ( XM_009292558.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290833.1 Danio rerio zinc finger protein 827 (znf827), transcript variant X3, mRNA.

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CloneID	ODa44462
Clone ID Related Accession (Same CDS sequence)	XM_009292558.2 , XM_009292558.3
Accession Version	XM_009292558.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	zinc finger protein 827 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009292558.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GGAAACAGGA GCAACCCAAG CGCCTGCCTT CACATGTGGA CACAGTAGAG 
GATGGAGGGC CAGAGAGTGG CTCAGTAGAG GAGGACAGCT GGTTTGGGAA TACCTCCGAC 
AGCCCCTCGG AATCGTCCTA CGGTGATGTG CAAGATGAGC TTCGGGTTTC TCCAGACAAC 
GTTCCCCGAC CGGACCACGA CACCTCAGCT GAGAGCTCCC TGGGCTGCCC GACGCCAGTT 
CAGCGGGCCT CTCTGGAGCT TCTGGGCCTG GACAGTGGGG TCCTTTCGAC CCAGGACACG 
CTTTCCTCTG TGGTGTCCTC TCTTTATGGA GACTCTAGCC AGGCTCTGGG TAAGCCACTC 
AGCAGTAATC TGCGCCGCCT CCTGGAGGCT GGCTCACTGA AGCTTGAGGG AGGGGAGCTG 
GCTGGACGTG GAACGGGTAG AGGACAAGAG TCACCCCCGG TCAGCCTGGT ACTATCTCCG 
TCTTCACATC ACGCACAGCA GCTGAGTGCC CTGGCGAGGA GGTTGGCAAA TAGCAGCAAC 
AACAGCGGAA ATTCAAACTC TGCGTCACCT GCATCTGTAG CAACTGTCAA GCAGGAGCCC 
TGTGACCCAT ACGGCCCCAG CAACGGCCCT AACTCAGGCA GCAGTGGCTT TGTATGGGTA 
GGCGTCGCAG ATAAATGGCC ATCGACAAGT TGCTCAGCAC CCAGTGGCAG TAGCCTCTCA 
CCAGACTCAG CAATTCAGAA GTTAAAGGCA GCCGCCAACG CCGTGCTCCA AGACAAGAAC 
GCGGTGGCCT CCTCCACCTC GGCATCTTCA TCCTCCTCGT CCTCCTCCTC ATCCTCTTCA 
GTCACCTCCT CCTCAATGGC AGCCCTGGGC ACTCGCTCTG ATGACGCAGT GCGCTTTGAA 
CCATTCGCTT CACCCTTTAG CCCGCAGTCA GCCAGCTCTA CGCTTGCTGC CCTCTCAAAG 
AAAGTGTCTG AAAGAAGTCA GCAGCAGGTG GGCTCATCTG AGCATCAACC CTCAGCTGGC 
TCCTTTCTCT CTCTGGTTTC CATGACCTCC TCAGCAGCTC TGCTAAAGGA GGTGGCCGCA 
CGAGCAGCAG GGAATCTTCT TGCTGATAAG AAAGAGCCGG CGTTAGTACT GGGAGCCTCC 
GAAGATGTCA AGCCCCTGTT AGACAGGAAC CAGAAGGTGC CCACCCCAAC CCAGGCAATG 
GATCTTCTGC TGCCAACAGT CCCCAAGGGA AGGGCCAAGC CCAGCAGTCA AGCAGGTTCT 
CCTGAGGATG GAGGAAAGCC CTTTCAGTGT CCGGTATGCG GTCTGGTCAT CAAGAGAAAG 
AGCTACTGGA AGAGACATAT GGTGATCCAT ACAGGCTTAA AAAGCCACCA GTGTCCCCTC 
TGTCCCTTTC GCTGTGCTCG TAAAGACAAT CTCAAGTCCC ACATGAAGGT CCATCAGCAC 
CAAGACCGAG GGGAGACGTT CCAGTGTGAG CTCTGCCCCT TCACATCCTC CCGCCACTTT 
AGCCTCAAGC TGCACATGCG TTGCCATCAG CACTTCCCTC GTCCCGACCT CAAAGTCAAA 
GAAGAACCCG GCACAGACAC CGAGGAGGGC GAGGGCTTGC TCATGGGGGA CGGTGGAGCG 
GCTGGTGATC AGGCCATGAA CACAGATGCT TCTACGTCTC CCACTGTGAG CCAACCCGAC 
GCTCGTTTGC TGGCCTCTCC TGCAGAACCC TCAAACCACG TCCAGATCAA GGAGGAACCA 
CAGGAGAGGG ATGTGTCTGT GATGTCTCCC TTTGCCCTGT GTAGGGAGAG GCCGAGCAGC 
AGCAGCAGTT CTCTGGATCT GTCCGGGCTT AAATCTAGTC CACCGGGGCC CGGGCCTACA 
GCTGCTTCAC TCTTCAGCCC AGATATCACC ACCAAGACGG CTACAGACCT CCTCATAAAG 
CTCTCGGCAG CGAACCAGAA GGAGGCCCTA AAGGGTCCAT ACTGTGTCAA GGAAGAGCCC 
CAGGCGGAGG AGCCCCTGAA CTCTCAGAGG GGACCCAGCT ACAGCTTCTG CCAAGAAGGA 
CCTCCAGGAG CCACCAAACC AAGAGAGGAG GGGAGCCCGA TGTTGGGCAA GAGCAGCCTT 
CTCAGCCAGG ACATCAACGT CAAAGTGGCC TCTGAATTGC TTATGAAGCT CTCAGAGAAC 
AACAAAGACG CTTGCGTCCA GAATGTTATT GTGAAGGCGG AGCCTATGGA AGTGGATCCA 
CCCCTTGATC TGGCCTCACC CTCCTCCCAT CTACTGGGCT TTAGCACTTC AGGACCATGT 
GAAAAGAAGG AACCAATGGT CAACCTCCCA GATCATTTGC CCCGCCCCCA GAATGACCTC 
TTCTCGCAGG ACATATCGGT CAAAATGGCC TCCGAGCTGC TTTTCAAACT CTCTGAAAAA 
GTAAGTAAAG CTAATGAAAA CAAAGACAAT GCATCATTCG GTATACACAG CCCTTTCCTT 
GATGACCGCT TTAGACAATC ACCTTTCAGC ACTCGCTCCA AGAGCTCTTC CCCAGCTGAA 
GCCGGATCTT CAAGCAGGGT TCCCCAGCAA GATCCAGAGA AGGTGAGTAG TGAGCCAGGA 
AATGGACTTT CCCAATGGAG ACTCAACGAA CAGCTCTTTC CCTGCCCTGT GTGTGGCAAA 
GTGTTTGGTC GCCAACAGAC TCTCTCCAGG CATCTCTCCT TACACACAGC TAAGTGCCGA 
GCCAACCTCA ACCAGCACCT CACCATCCAC TCAGTCAAAC TGGTGAACAC AGATGTCGAG 
CAGATTGTTA CCGCTGTTAC CAACGATGGC CCAGAGCTCA AAAACTGCCC CTACTACTAC 
AGCTGCCATG TCTGCGGCTT CCAGACAGAG TTCAATGCTC AGTTTGTCAG TCATATGTCT 
CTTCATGTGG ACAAAGAGCA GTGGATGTTT TCTCTCTGCT GTAGCACCTG TGAGTATGTG 
GGTGTGGACG AGGCCGAGAT GAAAGCTCAC ATCAGTGCAG GGCACGCAGG TCTCAATTCT 
AGAAGCCCTC TCAGTGAGAC CAAGAGCACT TCCTCCTCTC TGTCAGCCCT CAGCGACTCC 
CTCAACAGCT CTGAAGGTGG AGATGTTGCC CAAGGTAACG AAGAACTAAA AAGCCTTTTG 
GCTCCGCCTT CATCTGTGGG AAGCCAATCA AGCTTAGGCC CAGGAGCAGA GGAAAAAGCA 
GAGAAAGGCT TTGAATGCGT CTTCTGCAAC TTTGTGTGTA AAACCCGACC CATGTACGAG 
CGGCACCTAC AGATCCACCT GATCACGCGC ATGTTTGAGT GTGACGTCTG CCACAAGTTC 
ATGAAGACGC CCGAGCAGCT CCTGGAGCAC AAGAAGTGCC ACACCGTCCC CGCTGGAGGG 
CTCAAGTGCC CTCTCTGCAT CTACTCCACC AACAGGCCAG CCGCCATGGA GGGCCACCTG 
AAGACACACT ACAAGATGGA GTATCGCTGC CGCATCTGCC AAGCTGTGTG GGCCAATCAG 
GCAGAGCTGG AAAGGCACAC GCGCGCTCAC CGCCTGGGCA ACCACTATAA GTGTGAGCAG 
TGCGGCTACC TCTCCAAAAC AGCCAACAAG CTCATCGAGC ATGTGCGCGT GCACACCGGC 
GAGCGGCCTT TCCACTGCGA CCGCTGCAGC TACAGCTGCA AACGCAAGGA CAATCTCAAC 
CTCCACAAGC GGCTCAAGCA CGCACCGAGA CAGACCTTCG GTTGCGCCCA GTGCCCATTC 
ACCACCACTC ATCCGTTCGT CTACAGCCGC CATGTCAAAA AGCACCAAGG CGGGGAGGGA 
AGCGCCGAGG AAGGCGAGGC TCGGCCAGGT ACGCCCCATC CGGGCCTTCT GGAGCGAGGT 
GGGAGCAGCA GCGGGAGCGG GGACAGTGGG AGCACCAGTC CGCTCATGAA CCTGTCGGCC 
TCTCAAGCCT TGCAGTCCGT CGCCCTGTCA ATCACCAGCA AGCAGCAAGA TGGCACGCCA 
ACCGCGCGCT ACCTCTCAGC CAATGGCACG TTGTCCACAC TGGCGGCTCG CTACTTTGAC 
CAGTACAGGA ACTGTGACCT GGCTCACCTC GTCCCCCTCA CCACGCTCTT TACCACCCGA 
CGCTTCACAT TCCACAGCCC CTCGCCCTCA AACCCTTCGC CGCCCTTCTC TGCATTCTGG 
TCCAACTCAT CCCCGCAGTC ACCACCCTCT CCCACATCAC TCAAACACTC CTTCCTGGCA 
TATCTGGGAC TGACAGAGAG AGCGAAGACC ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290833.1
CDS	856..5091
Translation	MPRRKQEQPKRLPSHVDTVEDGGPESGSVEEDSWFGNTSDSPSESSYGDVQDELRVSPDNVPRPDHDTSAESSLGCPTPVQRASLELLGLDSGVLSTQDTLSSVVSSLYGDSSQALGKPLSSNLRRLLEAGSLKLEGGELAGRGTGRGQESPPVSLVLSPSSHHAQQLSALARRLANSSNNSGNSNSASPASVATVKQEPCDPYGPSNGPNSGSSGFVWVGVADKWPSTSCSAPSGSSLSPDSAIQKLKAAANAVLQDKNAVASSTSASSSSSSSSSSSSVTSSSMAALGTRSDDAVRFEPFASPFSPQSASSTLAALSKKVSERSQQQVGSSEHQPSAGSFLSLVSMTSSAALLKEVAARAAGNLLADKKEPALVLGASEDVKPLLDRNQKVPTPTQAMDLLLPTVPKGRAKPSSQAGSPEDGGKPFQCPVCGLVIKRKSYWKRHMVIHTGLKSHQCPLCPFRCARKDNLKSHMKVHQHQDRGETFQCELCPFTSSRHFSLKLHMRCHQHFPRPDLKVKEEPGTDTEEGEGLLMGDGGAAGDQAMNTDASTSPTVSQPDARLLASPAEPSNHVQIKEEPQERDVSVMSPFALCRERPSSSSSSLDLSGLKSSPPGPGPTAASLFSPDITTKTATDLLIKLSAANQKEALKGPYCVKEEPQAEEPLNSQRGPSYSFCQEGPPGATKPREEGSPMLGKSSLLSQDINVKVASELLMKLSENNKDACVQNVIVKAEPMEVDPPLDLASPSSHLLGFSTSGPCEKKEPMVNLPDHLPRPQNDLFSQDISVKMASELLFKLSEKVSKANENKDNASFGIHSPFLDDRFRQSPFSTRSKSSSPAEAGSSSRVPQQDPEKVSSEPGNGLSQWRLNEQLFPCPVCGKVFGRQQTLSRHLSLHTAKCRANLNQHLTIHSVKLVNTDVEQIVTAVTNDGPELKNCPYYYSCHVCGFQTEFNAQFVSHMSLHVDKEQWMFSLCCSTCEYVGVDEAEMKAHISAGHAGLNSRSPLSETKSTSSSLSALSDSLNSSEGGDVAQGNEELKSLLAPPSSVGSQSSLGPGAEEKAEKGFECVFCNFVCKTRPMYERHLQIHLITRMFECDVCHKFMKTPEQLLEHKKCHTVPAGGLKCPLCIYSTNRPAAMEGHLKTHYKMEYRCRICQAVWANQAELERHTRAHRLGNHYKCEQCGYLSKTANKLIEHVRVHTGERPFHCDRCSYSCKRKDNLNLHKRLKHAPRQTFGCAQCPFTTTHPFVYSRHVKKHQGGEGSAEEGEARPGTPHPGLLERGGSSSGSGDSGSTSPLMNLSASQALQSVALSITSKQQDGTPTARYLSANGTLSTLAARYFDQYRNCDLAHLVPLTTLFTTRRFTFHSPSPSNPSPPFSAFWSNSSPQSPPSPTSLKHSFLAYLGLTERAKTI

Target ORF information:

RefSeq Version	XM_009292558.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio zinc finger protein 827 (znf827), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292558.2

ORF Insert Sequence:

ATGCCAAGGA GGAAACAGGA GCAACCCAAG CGCCTGCCTT CACATGTGGA CACAGTAGAG 
GATGGAGGGC CAGAGAGTGG CTCAGTAGAG GAGGACAGCT GGTTTGGGAA TACCTCCGAC 
AGCCCCTCGG AATCGTCCTA CGGTGATGTG CAAGATGAGC TTCGGGTTTC TCCAGACAAC 
GTTCCCCGAC CGGACCACGA CACCTCAGCT GAGAGCTCCC TGGGCTGCCC GACGCCAGTT 
CAGCGGGCCT CTCTGGAGCT TCTGGGCCTG GACAGTGGGG TCCTTTCGAC CCAGGACACG 
CTTTCCTCTG TGGTGTCCTC TCTTTATGGA GACTCTAGCC AGGCTCTGGG TAAGCCACTC 
AGCAGTAATC TGCGCCGCCT CCTGGAGGCT GGCTCACTGA AGCTTGAGGG AGGGGAGCTG 
GCTGGACGTG GAACGGGTAG AGGACAAGAG TCACCCCCGG TCAGCCTGGT ACTATCTCCG 
TCTTCACATC ACGCACAGCA GCTGAGTGCC CTGGCGAGGA GGTTGGCAAA TAGCAGCAAC 
AACAGCGGAA ATTCAAACTC TGCGTCACCT GCATCTGTAG CAACTGTCAA GCAGGAGCCC 
TGTGACCCAT ACGGCCCCAG CAACGGCCCT AACTCAGGCA GCAGTGGCTT TGTATGGGTA 
GGCGTCGCAG ATAAATGGCC ATCGACAAGT TGCTCAGCAC CCAGTGGCAG TAGCCTCTCA 
CCAGACTCAG CAATTCAGAA GTTAAAGGCA GCCGCCAACG CCGTGCTCCA AGACAAGAAC 
GCGGTGGCCT CCTCCACCTC GGCATCTTCA TCCTCCTCGT CCTCCTCCTC ATCCTCTTCA 
GTCACCTCCT CCTCAATGGC AGCCCTGGGC ACTCGCTCTG ATGACGCAGT GCGCTTTGAA 
CCATTCGCTT CACCCTTTAG CCCGCAGTCA GCCAGCTCTA CGCTTGCTGC CCTCTCAAAG 
AAAGTGTCTG AAAGAAGTCA GCAGCAGGTG GGCTCATCTG AGCATCAACC CTCAGCTGGC 
TCCTTTCTCT CTCTGGTTTC CATGACCTCC TCAGCAGCTC TGCTAAAGGA GGTGGCCGCA 
CGAGCAGCAG GGAATCTTCT TGCTGATAAG AAAGAGCCGG CGTTAGTACT GGGAGCCTCC 
GAAGATGTCA AGCCCCTGTT AGACAGGAAC CAGAAGGTGC CCACCCCAAC CCAGGCAATG 
GATCTTCTGC TGCCAACAGT CCCCAAGGGA AGGGCCAAGC CCAGCAGTCA AGCAGGTTCT 
CCTGAGGATG GAGGAAAGCC CTTTCAGTGT CCGGTATGCG GTCTGGTCAT CAAGAGAAAG 
AGCTACTGGA AGAGACATAT GGTGATCCAT ACAGGCTTAA AAAGCCACCA GTGTCCCCTC 
TGTCCCTTTC GCTGTGCTCG TAAAGACAAT CTCAAGTCCC ACATGAAGGT CCATCAGCAC 
CAAGACCGAG GGGAGACGTT CCAGTGTGAG CTCTGCCCCT TCACATCCTC CCGCCACTTT 
AGCCTCAAGC TGCACATGCG TTGCCATCAG CACTTCCCTC GTCCCGACCT CAAAGTCAAA 
GAAGAACCCG GCACAGACAC CGAGGAGGGC GAGGGCTTGC TCATGGGGGA CGGTGGAGCG 
GCTGGTGATC AGGCCATGAA CACAGATGCT TCTACGTCTC CCACTGTGAG CCAACCCGAC 
GCTCGTTTGC TGGCCTCTCC TGCAGAACCC TCAAACCACG TCCAGATCAA GGAGGAACCA 
CAGGAGAGGG ATGTGTCTGT GATGTCTCCC TTTGCCCTGT GTAGGGAGAG GCCGAGCAGC 
AGCAGCAGTT CTCTGGATCT GTCCGGGCTT AAATCTAGTC CACCGGGGCC CGGGCCTACA 
GCTGCTTCAC TCTTCAGCCC AGATATCACC ACCAAGACGG CTACAGACCT CCTCATAAAG 
CTCTCGGCAG CGAACCAGAA GGAGGCCCTA AAGGGTCCAT ACTGTGTCAA GGAAGAGCCC 
CAGGCGGAGG AGCCCCTGAA CTCTCAGAGG GGACCCAGCT ACAGCTTCTG CCAAGAAGGA 
CCTCCAGGAG CCACCAAACC AAGAGAGGAG GGGAGCCCGA TGTTGGGCAA GAGCAGCCTT 
CTCAGCCAGG ACATCAACGT CAAAGTGGCC TCTGAATTGC TTATGAAGCT CTCAGAGAAC 
AACAAAGACG CTTGCGTCCA GAATGTTATT GTGAAGGCGG AGCCTATGGA AGTGGATCCA 
CCCCTTGATC TGGCCTCACC CTCCTCCCAT CTACTGGGCT TTAGCACTTC AGGACCATGT 
GAAAAGAAGG AACCAATGGT CAACCTCCCA GATCATTTGC CCCGCCCCCA GAATGACCTC 
TTCTCGCAGG ACATATCGGT CAAAATGGCC TCCGAGCTGC TTTTCAAACT CTCTGAAAAA 
GTAAGTAAAG CTAATGAAAA CAAAGACAAT GCATCATTCG GTATACACAG CCCTTTCCTT 
GATGACCGCT TTAGACAATC ACCTTTCAGC ACTCGCTCCA AGAGCTCTTC CCCAGCTGAA 
GCCGGATCTT CAAGCAGGGT TCCCCAGCAA GATCCAGAGA AGGTGAGTAG TGAGCCAGGA 
AATGGACTTT CCCAATGGAG ACTCAACGAA CAGCTCTTTC CCTGCCCTGT GTGTGGCAAA 
GTGTTTGGTC GCCAACAGAC TCTCTCCAGG CATCTCTCCT TACACACAGC TAAGTGCCGA 
GCCAACCTCA ACCAGCACCT CACCATCCAC TCAGTCAAAC TGGTGAACAC AGATGTCGAG 
CAGATTGTTA CCGCTGTTAC CAACGATGGC CCAGAGCTCA AAAACTGCCC CTACTACTAC 
AGCTGCCATG TCTGCGGCTT CCAGACAGAG TTCAATGCTC AGTTTGTCAG TCATATGTCT 
CTTCATGTGG ACAAAGAGCA GTGGATGTTT TCTCTCTGCT GTAGCACCTG TGAGTATGTG 
GGTGTGGACG AGGCCGAGAT GAAAGCTCAC ATCAGTGCAG GGCACGCAGG TCTCAATTCT 
AGAAGCCCTC TCAGTGAGAC CAAGAGCACT TCCTCCTCTC TGTCAGCCCT CAGCGACTCC 
CTCAACAGCT CTGAAGGTGG AGATGTTGCC CAAGGTAACG AAGAACTAAA AAGCCTTTTG 
GCTCCGCCTT CATCTGTGGG AAGCCAATCA AGCTTAGGCC CAGGAGCAGA GGAAAAAGCA 
GAGAAAGGCT TTGAATGCGT CTTCTGCAAC TTTGTGTGTA AAACCCGACC CATGTACGAG 
CGGCACCTAC AGATCCACCT GATCACGCGC ATGTTTGAGT GTGACGTCTG CCACAAGTTC 
ATGAAGACGC CCGAGCAGCT CCTGGAGCAC AAGAAGTGCC ACACCGTCCC CGCTGGAGGG 
CTCAAGTGCC CTCTCTGCAT CTACTCCACC AACAGGCCAG CCGCCATGGA GGGCCACCTG 
AAGACACACT ACAAGATGGA GTATCGCTGC CGCATCTGCC AAGCTGTGTG GGCCAATCAG 
GCAGAGCTGG AAAGGCACAC GCGCGCTCAC CGCCTGGGCA ACCACTATAA GTGTGAGCAG 
TGCGGCTACC TCTCCAAAAC AGCCAACAAG CTCATCGAGC ATGTGCGCGT GCACACCGGC 
GAGCGGCCTT TCCACTGCGA CCGCTGCAGC TACAGCTGCA AACGCAAGGA CAATCTCAAC 
CTCCACAAGC GGCTCAAGCA CGCACCGAGA CAGACCTTCG GTTGCGCCCA GTGCCCATTC 
ACCACCACTC ATCCGTTCGT CTACAGCCGC CATGTCAAAA AGCACCAAGG CGGGGAGGGA 
AGCGCCGAGG AAGGCGAGGC TCGGCCAGGT ACGCCCCATC CGGGCCTTCT GGAGCGAGGT 
GGGAGCAGCA GCGGGAGCGG GGACAGTGGG AGCACCAGTC CGCTCATGAA CCTGTCGGCC 
TCTCAAGCCT TGCAGTCCGT CGCCCTGTCA ATCACCAGCA AGCAGCAAGA TGGCACGCCA 
ACCGCGCGCT ACCTCTCAGC CAATGGCACG TTGTCCACAC TGGCGGCTCG CTACTTTGAC 
CAGTACAGGA ACTGTGACCT GGCTCACCTC GTCCCCCTCA CCACGCTCTT TACCACCCGA 
CGCTTCACAT TCCACAGCCC CTCGCCCTCA AACCCTTCGC CGCCCTTCTC TGCATTCTGG 
TCCAACTCAT CCCCGCAGTC ACCACCCTCT CCCACATCAC TCAAACACTC CTTCCTGGCA 
TATCTGGGAC TGACAGAGAG AGCGAAGACC ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

znf827 ( XM_009292558.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009290833.1 Danio rerio zinc finger protein 827 (znf827), transcript variant X3, mRNA.

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CloneID	ODa44462
Clone ID Related Accession (Same CDS sequence)	XM_009292558.2 , XM_009292558.3
Accession Version	XM_009292558.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	zinc finger protein 827 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009292558.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAGGA GGAAACAGGA GCAACCCAAG CGCCTGCCTT CACATGTGGA CACAGTAGAG 
GATGGAGGGC CAGAGAGTGG CTCAGTAGAG GAGGACAGCT GGTTTGGGAA TACCTCCGAC 
AGCCCCTCGG AATCGTCCTA CGGTGATGTG CAAGATGAGC TTCGGGTTTC TCCAGACAAC 
GTTCCCCGAC CGGACCACGA CACCTCAGCT GAGAGCTCCC TGGGCTGCCC GACGCCAGTT 
CAGCGGGCCT CTCTGGAGCT TCTGGGCCTG GACAGTGGGG TCCTTTCGAC CCAGGACACG 
CTTTCCTCTG TGGTGTCCTC TCTTTATGGA GACTCTAGCC AGGCTCTGGG TAAGCCACTC 
AGCAGTAATC TGCGCCGCCT CCTGGAGGCT GGCTCACTGA AGCTTGAGGG AGGGGAGCTG 
GCTGGACGTG GAACGGGTAG AGGACAAGAG TCACCCCCGG TCAGCCTGGT ACTATCTCCG 
TCTTCACATC ACGCACAGCA GCTGAGTGCC CTGGCGAGGA GGTTGGCAAA TAGCAGCAAC 
AACAGCGGAA ATTCAAACTC TGCGTCACCT GCATCTGTAG CAACTGTCAA GCAGGAGCCC 
TGTGACCCAT ACGGCCCCAG CAACGGCCCT AACTCAGGCA GCAGTGGCTT TGTATGGGTA 
GGCGTCGCAG ATAAATGGCC ATCGACAAGT TGCTCAGCAC CCAGTGGCAG TAGCCTCTCA 
CCAGACTCAG CAATTCAGAA GTTAAAGGCA GCCGCCAACG CCGTGCTCCA AGACAAGAAC 
GCGGTGGCCT CCTCCACCTC GGCATCTTCA TCCTCCTCGT CCTCCTCCTC ATCCTCTTCA 
GTCACCTCCT CCTCAATGGC AGCCCTGGGC ACTCGCTCTG ATGACGCAGT GCGCTTTGAA 
CCATTCGCTT CACCCTTTAG CCCGCAGTCA GCCAGCTCTA CGCTTGCTGC CCTCTCAAAG 
AAAGTGTCTG AAAGAAGTCA GCAGCAGGTG GGCTCATCTG AGCATCAACC CTCAGCTGGC 
TCCTTTCTCT CTCTGGTTTC CATGACCTCC TCAGCAGCTC TGCTAAAGGA GGTGGCCGCA 
CGAGCAGCAG GGAATCTTCT TGCTGATAAG AAAGAGCCGG CGTTAGTACT GGGAGCCTCC 
GAAGATGTCA AGCCCCTGTT AGACAGGAAC CAGAAGGTGC CCACCCCAAC CCAGGCAATG 
GATCTTCTGC TGCCAACAGT CCCCAAGGGA AGGGCCAAGC CCAGCAGTCA AGCAGGTTCT 
CCTGAGGATG GAGGAAAGCC CTTTCAGTGT CCGGTATGCG GTCTGGTCAT CAAGAGAAAG 
AGCTACTGGA AGAGACATAT GGTGATCCAT ACAGGCTTAA AAAGCCACCA GTGTCCCCTC 
TGTCCCTTTC GCTGTGCTCG TAAAGACAAT CTCAAGTCCC ACATGAAGGT CCATCAGCAC 
CAAGACCGAG GGGAGACGTT CCAGTGTGAG CTCTGCCCCT TCACATCCTC CCGCCACTTT 
AGCCTCAAGC TGCACATGCG TTGCCATCAG CACTTCCCTC GTCCCGACCT CAAAGTCAAA 
GAAGAACCCG GCACAGACAC CGAGGAGGGC GAGGGCTTGC TCATGGGGGA CGGTGGAGCG 
GCTGGTGATC AGGCCATGAA CACAGATGCT TCTACGTCTC CCACTGTGAG CCAACCCGAC 
GCTCGTTTGC TGGCCTCTCC TGCAGAACCC TCAAACCACG TCCAGATCAA GGAGGAACCA 
CAGGAGAGGG ATGTGTCTGT GATGTCTCCC TTTGCCCTGT GTAGGGAGAG GCCGAGCAGC 
AGCAGCAGTT CTCTGGATCT GTCCGGGCTT AAATCTAGTC CACCGGGGCC CGGGCCTACA 
GCTGCTTCAC TCTTCAGCCC AGATATCACC ACCAAGACGG CTACAGACCT CCTCATAAAG 
CTCTCGGCAG CGAACCAGAA GGAGGCCCTA AAGGGTCCAT ACTGTGTCAA GGAAGAGCCC 
CAGGCGGAGG AGCCCCTGAA CTCTCAGAGG GGACCCAGCT ACAGCTTCTG CCAAGAAGGA 
CCTCCAGGAG CCACCAAACC AAGAGAGGAG GGGAGCCCGA TGTTGGGCAA GAGCAGCCTT 
CTCAGCCAGG ACATCAACGT CAAAGTGGCC TCTGAATTGC TTATGAAGCT CTCAGAGAAC 
AACAAAGACG CTTGCGTCCA GAATGTTATT GTGAAGGCGG AGCCTATGGA AGTGGATCCA 
CCCCTTGATC TGGCCTCACC CTCCTCCCAT CTACTGGGCT TTAGCACTTC AGGACCATGT 
GAAAAGAAGG AACCAATGGT CAACCTCCCA GATCATTTGC CCCGCCCCCA GAATGACCTC 
TTCTCGCAGG ACATATCGGT CAAAATGGCC TCCGAGCTGC TTTTCAAACT CTCTGAAAAA 
GTAAGTAAAG CTAATGAAAA CAAAGACAAT GCATCATTCG GTATACACAG CCCTTTCCTT 
GATGACCGCT TTAGACAATC ACCTTTCAGC ACTCGCTCCA AGAGCTCTTC CCCAGCTGAA 
GCCGGATCTT CAAGCAGGGT TCCCCAGCAA GATCCAGAGA AGGTGAGTAG TGAGCCAGGA 
AATGGACTTT CCCAATGGAG ACTCAACGAA CAGCTCTTTC CCTGCCCTGT GTGTGGCAAA 
GTGTTTGGTC GCCAACAGAC TCTCTCCAGG CATCTCTCCT TACACACAGC TAAGTGCCGA 
GCCAACCTCA ACCAGCACCT CACCATCCAC TCAGTCAAAC TGGTGAACAC AGATGTCGAG 
CAGATTGTTA CCGCTGTTAC CAACGATGGC CCAGAGCTCA AAAACTGCCC CTACTACTAC 
AGCTGCCATG TCTGCGGCTT CCAGACAGAG TTCAATGCTC AGTTTGTCAG TCATATGTCT 
CTTCATGTGG ACAAAGAGCA GTGGATGTTT TCTCTCTGCT GTAGCACCTG TGAGTATGTG 
GGTGTGGACG AGGCCGAGAT GAAAGCTCAC ATCAGTGCAG GGCACGCAGG TCTCAATTCT 
AGAAGCCCTC TCAGTGAGAC CAAGAGCACT TCCTCCTCTC TGTCAGCCCT CAGCGACTCC 
CTCAACAGCT CTGAAGGTGG AGATGTTGCC CAAGGTAACG AAGAACTAAA AAGCCTTTTG 
GCTCCGCCTT CATCTGTGGG AAGCCAATCA AGCTTAGGCC CAGGAGCAGA GGAAAAAGCA 
GAGAAAGGCT TTGAATGCGT CTTCTGCAAC TTTGTGTGTA AAACCCGACC CATGTACGAG 
CGGCACCTAC AGATCCACCT GATCACGCGC ATGTTTGAGT GTGACGTCTG CCACAAGTTC 
ATGAAGACGC CCGAGCAGCT CCTGGAGCAC AAGAAGTGCC ACACCGTCCC CGCTGGAGGG 
CTCAAGTGCC CTCTCTGCAT CTACTCCACC AACAGGCCAG CCGCCATGGA GGGCCACCTG 
AAGACACACT ACAAGATGGA GTATCGCTGC CGCATCTGCC AAGCTGTGTG GGCCAATCAG 
GCAGAGCTGG AAAGGCACAC GCGCGCTCAC CGCCTGGGCA ACCACTATAA GTGTGAGCAG 
TGCGGCTACC TCTCCAAAAC AGCCAACAAG CTCATCGAGC ATGTGCGCGT GCACACCGGC 
GAGCGGCCTT TCCACTGCGA CCGCTGCAGC TACAGCTGCA AACGCAAGGA CAATCTCAAC 
CTCCACAAGC GGCTCAAGCA CGCACCGAGA CAGACCTTCG GTTGCGCCCA GTGCCCATTC 
ACCACCACTC ATCCGTTCGT CTACAGCCGC CATGTCAAAA AGCACCAAGG CGGGGAGGGA 
AGCGCCGAGG AAGGCGAGGC TCGGCCAGGT ACGCCCCATC CGGGCCTTCT GGAGCGAGGT 
GGGAGCAGCA GCGGGAGCGG GGACAGTGGG AGCACCAGTC CGCTCATGAA CCTGTCGGCC 
TCTCAAGCCT TGCAGTCCGT CGCCCTGTCA ATCACCAGCA AGCAGCAAGA TGGCACGCCA 
ACCGCGCGCT ACCTCTCAGC CAATGGCACG TTGTCCACAC TGGCGGCTCG CTACTTTGAC 
CAGTACAGGA ACTGTGACCT GGCTCACCTC GTCCCCCTCA CCACGCTCTT TACCACCCGA 
CGCTTCACAT TCCACAGCCC CTCGCCCTCA AACCCTTCGC CGCCCTTCTC TGCATTCTGG 
TCCAACTCAT CCCCGCAGTC ACCACCCTCT CCCACATCAC TCAAACACTC CTTCCTGGCA 
TATCTGGGAC TGACAGAGAG AGCGAAGACC ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009290833.1
CDS	414..4649
Translation	MPRRKQEQPKRLPSHVDTVEDGGPESGSVEEDSWFGNTSDSPSESSYGDVQDELRVSPDNVPRPDHDTSAESSLGCPTPVQRASLELLGLDSGVLSTQDTLSSVVSSLYGDSSQALGKPLSSNLRRLLEAGSLKLEGGELAGRGTGRGQESPPVSLVLSPSSHHAQQLSALARRLANSSNNSGNSNSASPASVATVKQEPCDPYGPSNGPNSGSSGFVWVGVADKWPSTSCSAPSGSSLSPDSAIQKLKAAANAVLQDKNAVASSTSASSSSSSSSSSSSVTSSSMAALGTRSDDAVRFEPFASPFSPQSASSTLAALSKKVSERSQQQVGSSEHQPSAGSFLSLVSMTSSAALLKEVAARAAGNLLADKKEPALVLGASEDVKPLLDRNQKVPTPTQAMDLLLPTVPKGRAKPSSQAGSPEDGGKPFQCPVCGLVIKRKSYWKRHMVIHTGLKSHQCPLCPFRCARKDNLKSHMKVHQHQDRGETFQCELCPFTSSRHFSLKLHMRCHQHFPRPDLKVKEEPGTDTEEGEGLLMGDGGAAGDQAMNTDASTSPTVSQPDARLLASPAEPSNHVQIKEEPQERDVSVMSPFALCRERPSSSSSSLDLSGLKSSPPGPGPTAASLFSPDITTKTATDLLIKLSAANQKEALKGPYCVKEEPQAEEPLNSQRGPSYSFCQEGPPGATKPREEGSPMLGKSSLLSQDINVKVASELLMKLSENNKDACVQNVIVKAEPMEVDPPLDLASPSSHLLGFSTSGPCEKKEPMVNLPDHLPRPQNDLFSQDISVKMASELLFKLSEKVSKANENKDNASFGIHSPFLDDRFRQSPFSTRSKSSSPAEAGSSSRVPQQDPEKVSSEPGNGLSQWRLNEQLFPCPVCGKVFGRQQTLSRHLSLHTAKCRANLNQHLTIHSVKLVNTDVEQIVTAVTNDGPELKNCPYYYSCHVCGFQTEFNAQFVSHMSLHVDKEQWMFSLCCSTCEYVGVDEAEMKAHISAGHAGLNSRSPLSETKSTSSSLSALSDSLNSSEGGDVAQGNEELKSLLAPPSSVGSQSSLGPGAEEKAEKGFECVFCNFVCKTRPMYERHLQIHLITRMFECDVCHKFMKTPEQLLEHKKCHTVPAGGLKCPLCIYSTNRPAAMEGHLKTHYKMEYRCRICQAVWANQAELERHTRAHRLGNHYKCEQCGYLSKTANKLIEHVRVHTGERPFHCDRCSYSCKRKDNLNLHKRLKHAPRQTFGCAQCPFTTTHPFVYSRHVKKHQGGEGSAEEGEARPGTPHPGLLERGGSSSGSGDSGSTSPLMNLSASQALQSVALSITSKQQDGTPTARYLSANGTLSTLAARYFDQYRNCDLAHLVPLTTLFTTRRFTFHSPSPSNPSPPFSAFWSNSSPQSPPSPTSLKHSFLAYLGLTERAKTI

Target ORF information:

RefSeq Version	XM_009292558.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zinc finger protein 827 (znf827), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009292558.3

ORF Insert Sequence:

ATGCCAAGGA GGAAACAGGA GCAACCCAAG CGCCTGCCTT CACATGTGGA CACAGTAGAG 
GATGGAGGGC CAGAGAGTGG CTCAGTAGAG GAGGACAGCT GGTTTGGGAA TACCTCCGAC 
AGCCCCTCGG AATCGTCCTA CGGTGATGTG CAAGATGAGC TTCGGGTTTC TCCAGACAAC 
GTTCCCCGAC CGGACCACGA CACCTCAGCT GAGAGCTCCC TGGGCTGCCC GACGCCAGTT 
CAGCGGGCCT CTCTGGAGCT TCTGGGCCTG GACAGTGGGG TCCTTTCGAC CCAGGACACG 
CTTTCCTCTG TGGTGTCCTC TCTTTATGGA GACTCTAGCC AGGCTCTGGG TAAGCCACTC 
AGCAGTAATC TGCGCCGCCT CCTGGAGGCT GGCTCACTGA AGCTTGAGGG AGGGGAGCTG 
GCTGGACGTG GAACGGGTAG AGGACAAGAG TCACCCCCGG TCAGCCTGGT ACTATCTCCG 
TCTTCACATC ACGCACAGCA GCTGAGTGCC CTGGCGAGGA GGTTGGCAAA TAGCAGCAAC 
AACAGCGGAA ATTCAAACTC TGCGTCACCT GCATCTGTAG CAACTGTCAA GCAGGAGCCC 
TGTGACCCAT ACGGCCCCAG CAACGGCCCT AACTCAGGCA GCAGTGGCTT TGTATGGGTA 
GGCGTCGCAG ATAAATGGCC ATCGACAAGT TGCTCAGCAC CCAGTGGCAG TAGCCTCTCA 
CCAGACTCAG CAATTCAGAA GTTAAAGGCA GCCGCCAACG CCGTGCTCCA AGACAAGAAC 
GCGGTGGCCT CCTCCACCTC GGCATCTTCA TCCTCCTCGT CCTCCTCCTC ATCCTCTTCA 
GTCACCTCCT CCTCAATGGC AGCCCTGGGC ACTCGCTCTG ATGACGCAGT GCGCTTTGAA 
CCATTCGCTT CACCCTTTAG CCCGCAGTCA GCCAGCTCTA CGCTTGCTGC CCTCTCAAAG 
AAAGTGTCTG AAAGAAGTCA GCAGCAGGTG GGCTCATCTG AGCATCAACC CTCAGCTGGC 
TCCTTTCTCT CTCTGGTTTC CATGACCTCC TCAGCAGCTC TGCTAAAGGA GGTGGCCGCA 
CGAGCAGCAG GGAATCTTCT TGCTGATAAG AAAGAGCCGG CGTTAGTACT GGGAGCCTCC 
GAAGATGTCA AGCCCCTGTT AGACAGGAAC CAGAAGGTGC CCACCCCAAC CCAGGCAATG 
GATCTTCTGC TGCCAACAGT CCCCAAGGGA AGGGCCAAGC CCAGCAGTCA AGCAGGTTCT 
CCTGAGGATG GAGGAAAGCC CTTTCAGTGT CCGGTATGCG GTCTGGTCAT CAAGAGAAAG 
AGCTACTGGA AGAGACATAT GGTGATCCAT ACAGGCTTAA AAAGCCACCA GTGTCCCCTC 
TGTCCCTTTC GCTGTGCTCG TAAAGACAAT CTCAAGTCCC ACATGAAGGT CCATCAGCAC 
CAAGACCGAG GGGAGACGTT CCAGTGTGAG CTCTGCCCCT TCACATCCTC CCGCCACTTT 
AGCCTCAAGC TGCACATGCG TTGCCATCAG CACTTCCCTC GTCCCGACCT CAAAGTCAAA 
GAAGAACCCG GCACAGACAC CGAGGAGGGC GAGGGCTTGC TCATGGGGGA CGGTGGAGCG 
GCTGGTGATC AGGCCATGAA CACAGATGCT TCTACGTCTC CCACTGTGAG CCAACCCGAC 
GCTCGTTTGC TGGCCTCTCC TGCAGAACCC TCAAACCACG TCCAGATCAA GGAGGAACCA 
CAGGAGAGGG ATGTGTCTGT GATGTCTCCC TTTGCCCTGT GTAGGGAGAG GCCGAGCAGC 
AGCAGCAGTT CTCTGGATCT GTCCGGGCTT AAATCTAGTC CACCGGGGCC CGGGCCTACA 
GCTGCTTCAC TCTTCAGCCC AGATATCACC ACCAAGACGG CTACAGACCT CCTCATAAAG 
CTCTCGGCAG CGAACCAGAA GGAGGCCCTA AAGGGTCCAT ACTGTGTCAA GGAAGAGCCC 
CAGGCGGAGG AGCCCCTGAA CTCTCAGAGG GGACCCAGCT ACAGCTTCTG CCAAGAAGGA 
CCTCCAGGAG CCACCAAACC AAGAGAGGAG GGGAGCCCGA TGTTGGGCAA GAGCAGCCTT 
CTCAGCCAGG ACATCAACGT CAAAGTGGCC TCTGAATTGC TTATGAAGCT CTCAGAGAAC 
AACAAAGACG CTTGCGTCCA GAATGTTATT GTGAAGGCGG AGCCTATGGA AGTGGATCCA 
CCCCTTGATC TGGCCTCACC CTCCTCCCAT CTACTGGGCT TTAGCACTTC AGGACCATGT 
GAAAAGAAGG AACCAATGGT CAACCTCCCA GATCATTTGC CCCGCCCCCA GAATGACCTC 
TTCTCGCAGG ACATATCGGT CAAAATGGCC TCCGAGCTGC TTTTCAAACT CTCTGAAAAA 
GTAAGTAAAG CTAATGAAAA CAAAGACAAT GCATCATTCG GTATACACAG CCCTTTCCTT 
GATGACCGCT TTAGACAATC ACCTTTCAGC ACTCGCTCCA AGAGCTCTTC CCCAGCTGAA 
GCCGGATCTT CAAGCAGGGT TCCCCAGCAA GATCCAGAGA AGGTGAGTAG TGAGCCAGGA 
AATGGACTTT CCCAATGGAG ACTCAACGAA CAGCTCTTTC CCTGCCCTGT GTGTGGCAAA 
GTGTTTGGTC GCCAACAGAC TCTCTCCAGG CATCTCTCCT TACACACAGC TAAGTGCCGA 
GCCAACCTCA ACCAGCACCT CACCATCCAC TCAGTCAAAC TGGTGAACAC AGATGTCGAG 
CAGATTGTTA CCGCTGTTAC CAACGATGGC CCAGAGCTCA AAAACTGCCC CTACTACTAC 
AGCTGCCATG TCTGCGGCTT CCAGACAGAG TTCAATGCTC AGTTTGTCAG TCATATGTCT 
CTTCATGTGG ACAAAGAGCA GTGGATGTTT TCTCTCTGCT GTAGCACCTG TGAGTATGTG 
GGTGTGGACG AGGCCGAGAT GAAAGCTCAC ATCAGTGCAG GGCACGCAGG TCTCAATTCT 
AGAAGCCCTC TCAGTGAGAC CAAGAGCACT TCCTCCTCTC TGTCAGCCCT CAGCGACTCC 
CTCAACAGCT CTGAAGGTGG AGATGTTGCC CAAGGTAACG AAGAACTAAA AAGCCTTTTG 
GCTCCGCCTT CATCTGTGGG AAGCCAATCA AGCTTAGGCC CAGGAGCAGA GGAAAAAGCA 
GAGAAAGGCT TTGAATGCGT CTTCTGCAAC TTTGTGTGTA AAACCCGACC CATGTACGAG 
CGGCACCTAC AGATCCACCT GATCACGCGC ATGTTTGAGT GTGACGTCTG CCACAAGTTC 
ATGAAGACGC CCGAGCAGCT CCTGGAGCAC AAGAAGTGCC ACACCGTCCC CGCTGGAGGG 
CTCAAGTGCC CTCTCTGCAT CTACTCCACC AACAGGCCAG CCGCCATGGA GGGCCACCTG 
AAGACACACT ACAAGATGGA GTATCGCTGC CGCATCTGCC AAGCTGTGTG GGCCAATCAG 
GCAGAGCTGG AAAGGCACAC GCGCGCTCAC CGCCTGGGCA ACCACTATAA GTGTGAGCAG 
TGCGGCTACC TCTCCAAAAC AGCCAACAAG CTCATCGAGC ATGTGCGCGT GCACACCGGC 
GAGCGGCCTT TCCACTGCGA CCGCTGCAGC TACAGCTGCA AACGCAAGGA CAATCTCAAC 
CTCCACAAGC GGCTCAAGCA CGCACCGAGA CAGACCTTCG GTTGCGCCCA GTGCCCATTC 
ACCACCACTC ATCCGTTCGT CTACAGCCGC CATGTCAAAA AGCACCAAGG CGGGGAGGGA 
AGCGCCGAGG AAGGCGAGGC TCGGCCAGGT ACGCCCCATC CGGGCCTTCT GGAGCGAGGT 
GGGAGCAGCA GCGGGAGCGG GGACAGTGGG AGCACCAGTC CGCTCATGAA CCTGTCGGCC 
TCTCAAGCCT TGCAGTCCGT CGCCCTGTCA ATCACCAGCA AGCAGCAAGA TGGCACGCCA 
ACCGCGCGCT ACCTCTCAGC CAATGGCACG TTGTCCACAC TGGCGGCTCG CTACTTTGAC 
CAGTACAGGA ACTGTGACCT GGCTCACCTC GTCCCCCTCA CCACGCTCTT TACCACCCGA 
CGCTTCACAT TCCACAGCCC CTCGCCCTCA AACCCTTCGC CGCCCTTCTC TGCATTCTGG 
TCCAACTCAT CCCCGCAGTC ACCACCCTCT CCCACATCAC TCAAACACTC CTTCCTGGCA 
TATCTGGGAC TGACAGAGAG AGCGAAGACC ATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	znf827
Entrez Gene ID	565077
Full Name	zinc finger protein 827
Synonyms	si:ch211-215c3.2
General protein information	Preferred Names zinc finger protein 827 Names zinc finger protein 827
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Danio rerio (zebrafish)	znf827	XP_005160034.1
Bos taurus (cattle)	ZNF827	XP_005217571.1
Mus musculus (house mouse)	Zfp827	NP_839998.2
Pan troglodytes (chimpanzee)	ZNF827	XP_517584.3
Homo sapiens (human)	ZNF827	NP_849157.2
Canis lupus familiaris (dog)	ZNF827	XP_005629371.1
Rattus norvegicus (Norway rat)	Zfp827	XP_006255475.1
Xenopus tropicalis (tropical clawed frog)	znf827	XP_002938981.2
	ZNF827	XP_001094345.1
Gallus gallus (chicken)	ZNF827	XP_004941016.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

znf827 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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