pank1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pank1b
Entrez Gene ID	565059
Full Name	pantothenate kinase 1b
Synonyms	im:7149302,wu:fb51a11,zgc:158746
General protein information	Preferred Names pantothenate kinase 1b Names pantothenate kinase 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021480132.1	XP_021335807.1	pantothenate kinase 1 isoform X1
NM_001083841.1	NP_001077310.1	pantothenate kinase 1

Pathways from BioSystems

Homologies

	PANK1	XP_001087260.1
Bos taurus (cattle)	PANK1	XP_002698391.1
Rattus norvegicus (Norway rat)	Pank1	NP_001099843.1
Danio rerio (zebrafish)	pank1b	NP_001077310.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011715	XP_320797.3
Mus musculus (house mouse)	Pank1	NP_001107811.1
Pan troglodytes (chimpanzee)	PANK1	XP_001143095.1
Drosophila melanogaster (fruit fly)	fbl	NP_524870.1
Xenopus tropicalis (tropical clawed frog)	pank1	XP_004915939.1
Homo sapiens (human)	PANK1	NP_683878.1

Gene Ontology
Bibliography

Related articles in PubMed

Knock-down of pantothenate kinase 2 severely affects the development of the nervous and vascular system in zebrafish, providing new insights into PKAN disease.
Zizioli D, Tiso N, Guglielmi A, Saraceno C, Busolin G, Giuliani R, Khatri D, Monti E, Borsani G, Argenton F, Finazzi D
Neurobiology of disease8535-48(2016 Jan)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Systematic transcriptome analysis of the zebrafish model of diamond-blackfan anemia induced by RPS24 deficiency.
Song B, Zhang Q, Zhang Z, Wan Y, Jia Q, Wang X, Zhu X, Leung AY, Cheng T, Fang X, Yuan W, Jia H
BMC genomics15759(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following pank1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pank1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa34850	NM_001083841.1 Latest version!	Danio rerio pantothenate kinase 1b (pank1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa148208	XM_021480132.1 Latest version!	Danio rerio pantothenate kinase 1b (pank1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pank1b ( NM_001083841.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001077310.1 Danio rerio pantothenate kinase 1b (pank1b), mRNA.

Price & Availability↑

CloneID	ODa34850
Clone ID Related Accession (Same CDS sequence)	NM_001083841.1
Accession Version	NM_001083841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-19
Organism	Danio rerio(zebrafish)
Product	pantothenate kinase 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC133149.1. On Apr 5, 2007 this sequence version replaced XM_681962.2. ##Evidence-Data-START## Transcript exon combination :: BC133149.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGAAAATG AAGAAACATC CGATCAAAAC GGAACGACGC ACTACAAAAA AGGTTTAGTA 
AACGGATTTC ACCCCGCACA GTCGGGTAAC GGCGATTGCA TTTCGTCCAA CGGAGGTGGT 
CTGGGTACTA GTGACAGTAG CGGTGGCCAT AATGGTATTG AAAATTTCCA CCATCTTCAT 
CAGGATGTAC ACAACAATGG TTCGCCTGCG AAGCGCTGCA GACTCAGAAG ACGAGTGGAT 
TCGGGAAAGA AGAACAGACC ACCCTTTCCG TGGTTTGGCA TGGATATTGG AGGCACCTTG 
GTGAAGCTGG TTTATTTTGA GCCCAAGGAC ATAACAGCTG AAGAAGAACA AGAGGAAGTG 
GAGAGCCTGA AGAGCATCCG CTGGTACCTG ACATCCAACG TTGCCTATGG AAACACTGGA 
ATCCGTGATG TTCACCTGGA GTTGAAGAAC CTCACCATGT GCGGCCGCAA AGGCAACCTG 
CACTTCATTC GGTTCCCCAC ACAAGACATG CACCGCTTTA TCCAAATGGG CCGAGACAAG 
AACTTCTCTA GTCTGCACAC CACTCTATGC GCCACCGGTG GGGGGGCGTA CAAATTTGAG 
GATGACTTCA GAACGGTGGC TGATTTGGAG CTGCAGAAGT TGGATGAACT AGATTGTCTT 
ATTCAAGGTC TGCTGTACGT TGATTCAGTC GGGTTCAATG GCCATCCAGA ATGTTATTTT 
TTCCAAAATC CATCTGAACC TGAAAACTGT GTCAAGAGGC CATTTAGCCT GGAGAACCCT 
CTCCCTATGT TGCTGGTAAA TATTGGCTCT GGCGTTAGTA TCCTGGCCGT CTATTCCAAG 
GATGATTACA AGCGGGTCAC AGGCACCAGT TTAGGAGGTG GTACCTTTCT TGGCCTGTGC 
TGCTTGCTGA CTGGCTGTGA AACATTTGAG GAGGCATTAG AAATGGCAGC TAAAGGGGAC 
AGCACTAACG TGGACAAACT AGTGAAGGAT ATTTATGGAG GCGATTATGA ACGATTTGGC 
CTTCAGGGTT CGGCCGTTGC ATCTAGTTTT TGTCATATGA TGAGCAAGGA GAAACGCGAT 
AGTATAAGTA AAGAAGACCT GGCCAGGGCG ACTCTTGTCA CCATAACAAA CAACATTGGC 
TCCATCGCTC GCATGTGTGC AGTGAACGAG AATATCGATA AAGTGGTTTT TGTTGGAAAT 
TTCCTTCGGA TCAACACCAT TTCAACTAAG CTGCTGGCCT ATGCCATGGA CTTCTGGTCT 
GATGGACAAC TGAAAGCTCT TTTCTTAGAA CATGAAGGTT ATTTTGGAGC TGTCGGTGCC 
TTCCTAGAGC TGCTGAAGAT GACAGAAGAT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001077310.1
CDS	17..1372
Translation	MENEETSDQNGTTHYKKGLVNGFHPAQSGNGDCISSNGGGLGTSDSSGGHNGIENFHHLHQDVHNNGSPAKRCRLRRRVDSGKKNRPPFPWFGMDIGGTLVKLVYFEPKDITAEEEQEEVESLKSIRWYLTSNVAYGNTGIRDVHLELKNLTMCGRKGNLHFIRFPTQDMHRFIQMGRDKNFSSLHTTLCATGGGAYKFEDDFRTVADLELQKLDELDCLIQGLLYVDSVGFNGHPECYFFQNPSEPENCVKRPFSLENPLPMLLVNIGSGVSILAVYSKDDYKRVTGTSLGGGTFLGLCCLLTGCETFEEALEMAAKGDSTNVDKLVKDIYGGDYERFGLQGSAVASSFCHMMSKEKRDSISKEDLARATLVTITNNIGSIARMCAVNENIDKVVFVGNFLRINTISTKLLAYAMDFWSDGQLKALFLEHEGYFGAVGAFLELLKMTEDS

Target ORF information:

RefSeq Version	NM_001083841.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pantothenate kinase 1b (pank1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083841.1

ORF Insert Sequence:

ATGGAAAATG AAGAAACATC CGATCAAAAC GGAACGACGC ACTACAAAAA AGGTTTAGTA 
AACGGATTTC ACCCCGCACA GTCGGGTAAC GGCGATTGCA TTTCGTCCAA CGGAGGTGGT 
CTGGGTACTA GTGACAGTAG CGGTGGCCAT AATGGTATTG AAAATTTCCA CCATCTTCAT 
CAGGATGTAC ACAACAATGG TTCGCCTGCG AAGCGCTGCA GACTCAGAAG ACGAGTGGAT 
TCGGGAAAGA AGAACAGACC ACCCTTTCCG TGGTTTGGCA TGGATATTGG AGGCACCTTG 
GTGAAGCTGG TTTATTTTGA GCCCAAGGAC ATAACAGCTG AAGAAGAACA AGAGGAAGTG 
GAGAGCCTGA AGAGCATCCG CTGGTACCTG ACATCCAACG TTGCCTATGG AAACACTGGA 
ATCCGTGATG TTCACCTGGA GTTGAAGAAC CTCACCATGT GCGGCCGCAA AGGCAACCTG 
CACTTCATTC GGTTCCCCAC ACAAGACATG CACCGCTTTA TCCAAATGGG CCGAGACAAG 
AACTTCTCTA GTCTGCACAC CACTCTATGC GCCACCGGTG GGGGGGCGTA CAAATTTGAG 
GATGACTTCA GAACGGTGGC TGATTTGGAG CTGCAGAAGT TGGATGAACT AGATTGTCTT 
ATTCAAGGTC TGCTGTACGT TGATTCAGTC GGGTTCAATG GCCATCCAGA ATGTTATTTT 
TTCCAAAATC CATCTGAACC TGAAAACTGT GTCAAGAGGC CATTTAGCCT GGAGAACCCT 
CTCCCTATGT TGCTGGTAAA TATTGGCTCT GGCGTTAGTA TCCTGGCCGT CTATTCCAAG 
GATGATTACA AGCGGGTCAC AGGCACCAGT TTAGGAGGTG GTACCTTTCT TGGCCTGTGC 
TGCTTGCTGA CTGGCTGTGA AACATTTGAG GAGGCATTAG AAATGGCAGC TAAAGGGGAC 
AGCACTAACG TGGACAAACT AGTGAAGGAT ATTTATGGAG GCGATTATGA ACGATTTGGC 
CTTCAGGGTT CGGCCGTTGC ATCTAGTTTT TGTCATATGA TGAGCAAGGA GAAACGCGAT 
AGTATAAGTA AAGAAGACCT GGCCAGGGCG ACTCTTGTCA CCATAACAAA CAACATTGGC 
TCCATCGCTC GCATGTGTGC AGTGAACGAG AATATCGATA AAGTGGTTTT TGTTGGAAAT 
TTCCTTCGGA TCAACACCAT TTCAACTAAG CTGCTGGCCT ATGCCATGGA CTTCTGGTCT 
GATGGACAAC TGAAAGCTCT TTTCTTAGAA CATGAAGGTT ATTTTGGAGC TGTCGGTGCC 
TTCCTAGAGC TGCTGAAGAT GACAGAAGAT TCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pank1b ( XM_021480132.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335807.1 Danio rerio pantothenate kinase 1b (pank1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa148208
Clone ID Related Accession (Same CDS sequence)	XM_021480132.1
Accession Version	XM_021480132.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	pantothenate kinase 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCTTA TTGTGAAGAA GCCAGCCTTT CCGTGGTTTG GCATGGATAT TGGAGGCACC 
TTGGTGAAGC TGGTTTATTT TGAGCCCAAG GACATAACAG CTGAAGAAGA ACAAGAGGAA 
GTGGAGAGCC TGAAGAGCAT CCGCTGGTAC CTGACATCCA ACGTTGCCTA TGGAAACACT 
GGAATCCGTG ATGTTCACCT GGAGTTGAAG AACCTCACCA TGTGCGGCCG CAAAGGCAAC 
CTGCACTTCA TTCGGTTCCC CACACAAGAC ATGCACCGCT TTATCCAAAT GGGCCGAGAC 
AAGAACTTCT CTAGTCTGCA CACCACTCTA TGCGCCACCG GTGGGGGGGC GTACAAATTT 
GAGGATGACT TCAGAACGGT GGCTGATTTG GAGCTGCAGA AGTTGGATGA ACTAGATTGT 
CTTATTCAAG GTCTGCTGTA CGTTGATTCA GTCGGGTTCA ATGGCCATCC AGAATGTTAT 
TTTTTCCAAA ATCCATCTGA ACCTGAAAAC TGTGTCAAGA GGCCATTTAG CCTGGAGAAC 
CCTTTCCCTA TGTTGCTGGT AAATATTGGC TCTGGCGTTA GTATCCTGGC CGTCTATTCC 
AAGGATGATT ACAAGCGGGT CACAGGCACC AGTTTAGGAG GTGGTACCTT TCTTGGCCTG 
TGCTGCTTGC TGACTGGCTG TGAAACATTT GAGGAGGCAT TAGAAATGGC GGCTAAAGGG 
GACAGCACTA ACGTGGACAA ACTAGTGAAG GATATTTATG GAGGCGATTA TGAACGATTT 
GGCCTTCAGG GTTCGGCCGT TGCATCTAGT TTTTGTCATA TGATGAGCAA GGAGAAACGC 
GATAGTATAA GTAAAGAAGA CCTGGCCAGG GCGACTCTTG TCACCATAAC AAACAACATT 
GGCTCCATCG CTCGCATGTG TGCAGTGAAC GAGAATATCG ATAAAGTGGT TTTTGTTGGA 
AATTTCCTTC GGATCAACAC CATTTCAACT AAGCTGCTGG CCTATGCCAT GGACTTCTGG 
TCTGATGGAC AACTGAAAGC TCTTTTCTTA GAACATGAAG GTTATTTTGG AGCTGTCGGT 
GCCTTCCTAG AGCTGCTGAA GATGACAGAA GATTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335807.1
CDS	228..1346
Translation	MKLIVKKPAFPWFGMDIGGTLVKLVYFEPKDITAEEEQEEVESLKSIRWYLTSNVAYGNTGIRDVHLELKNLTMCGRKGNLHFIRFPTQDMHRFIQMGRDKNFSSLHTTLCATGGGAYKFEDDFRTVADLELQKLDELDCLIQGLLYVDSVGFNGHPECYFFQNPSEPENCVKRPFSLENPFPMLLVNIGSGVSILAVYSKDDYKRVTGTSLGGGTFLGLCCLLTGCETFEEALEMAAKGDSTNVDKLVKDIYGGDYERFGLQGSAVASSFCHMMSKEKRDSISKEDLARATLVTITNNIGSIARMCAVNENIDKVVFVGNFLRINTISTKLLAYAMDFWSDGQLKALFLEHEGYFGAVGAFLELLKMTEDS

Target ORF information:

RefSeq Version	XM_021480132.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio pantothenate kinase 1b (pank1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021480132.1

ORF Insert Sequence:

ATGAAGCTTA TTGTGAAGAA GCCAGCCTTT CCGTGGTTTG GCATGGATAT TGGAGGCACC 
TTGGTGAAGC TGGTTTATTT TGAGCCCAAG GACATAACAG CTGAAGAAGA ACAAGAGGAA 
GTGGAGAGCC TGAAGAGCAT CCGCTGGTAC CTGACATCCA ACGTTGCCTA TGGAAACACT 
GGAATCCGTG ATGTTCACCT GGAGTTGAAG AACCTCACCA TGTGCGGCCG CAAAGGCAAC 
CTGCACTTCA TTCGGTTCCC CACACAAGAC ATGCACCGCT TTATCCAAAT GGGCCGAGAC 
AAGAACTTCT CTAGTCTGCA CACCACTCTA TGCGCCACCG GTGGGGGGGC GTACAAATTT 
GAGGATGACT TCAGAACGGT GGCTGATTTG GAGCTGCAGA AGTTGGATGA ACTAGATTGT 
CTTATTCAAG GTCTGCTGTA CGTTGATTCA GTCGGGTTCA ATGGCCATCC AGAATGTTAT 
TTTTTCCAAA ATCCATCTGA ACCTGAAAAC TGTGTCAAGA GGCCATTTAG CCTGGAGAAC 
CCTTTCCCTA TGTTGCTGGT AAATATTGGC TCTGGCGTTA GTATCCTGGC CGTCTATTCC 
AAGGATGATT ACAAGCGGGT CACAGGCACC AGTTTAGGAG GTGGTACCTT TCTTGGCCTG 
TGCTGCTTGC TGACTGGCTG TGAAACATTT GAGGAGGCAT TAGAAATGGC GGCTAAAGGG 
GACAGCACTA ACGTGGACAA ACTAGTGAAG GATATTTATG GAGGCGATTA TGAACGATTT 
GGCCTTCAGG GTTCGGCCGT TGCATCTAGT TTTTGTCATA TGATGAGCAA GGAGAAACGC 
GATAGTATAA GTAAAGAAGA CCTGGCCAGG GCGACTCTTG TCACCATAAC AAACAACATT 
GGCTCCATCG CTCGCATGTG TGCAGTGAAC GAGAATATCG ATAAAGTGGT TTTTGTTGGA 
AATTTCCTTC GGATCAACAC CATTTCAACT AAGCTGCTGG CCTATGCCAT GGACTTCTGG 
TCTGATGGAC AACTGAAAGC TCTTTTCTTA GAACATGAAG GTTATTTTGG AGCTGTCGGT 
GCCTTCCTAG AGCTGCTGAA GATGACAGAA GATTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Knock-down of pantothenate kinase 2 severely affects the development of the nervous and vascular system in zebrafish, providing new insights into PKAN disease.
Neurobiology of disease8535-48(2016 Jan)
Zizioli D,Tiso N,Guglielmi A,Saraceno C,Busolin G,Giuliani R,Khatri D,Monti E,Borsani G,Argenton F,Finazzi D

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Systematic transcriptome analysis of the zebrafish model of diamond-blackfan anemia induced by RPS24 deficiency.
BMC genomics15759(2014 Sep)
Song B,Zhang Q,Zhang Z,Wan Y,Jia Q,Wang X,Zhu X,Leung AY,Cheng T,Fang X,Yuan W,Jia H

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pank1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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